1,4,7,10-TETRAAZABICYCLO[8.2.2]TETRADECAN-2-ONE, A PROCESS FOR THE PREPARATION THEREOF AND THE USE THEREOF FOR THE PREPARATION OF TETRAAZAMACROCYCLES

Abstract: The present invention relates to the novel compound, 1,4,7,10-tetraazabicyclo[8.2.2]tetradecan-2-one of formula (I), its preparation and the use thereof for the preparation of tetraazamacrocycles.

Description: 1.4.7.1O-TETRAAZABICYCΓ.OΓB .2.21TETRADECAN-2-ONE . A PROCESS FOR THE PREPARATION THEREOF AND THE USE THEREOF FOR THE PREPARATION OF TETRAAZAMACROCYCLES The present invention relates to the novel compound, 1,4,7 , 10-tetraazabicyclo[8.2.2]tetradecan-2- one of formula (I), its preparation and the use thereof for the preparation of tetraazamacrocycles . The compound of formula (I) was surprisingly synthesized starting from compound of formula ( II ) , 2α,4α, 6α ,8α-decahydro-teatraazacyclopent [fg]acena- phthylene, already known as an intermediate for the preparation of polyazamacrocycles derivatives. The compound of formula (II) can, in its turn, be obtained starting from straight polyamines, as described in Italian Patent application MI 96A 001257, and it can be converted to 1 , 4 , 7 , 10-tetraazacyclododecane (commonly named Cyclen) according to the following scheme: + glyoxal c) oxidation d) hydrolysis in which step a) is the condensation of triethylenetriamine with a glyoxal derivative (glyoxal hydrate, or Bertagnini's salt), in water or in water- soluble solvents or in mixtures thereof, at temperatures from 0 to 50"C, in the presence of εtoichiometric amounts or in a slight excess of calcium hydroxide; step b) is the condensation of the resulting compound from step a) with an X-CH2-CH2- alkylating agent, in which X is a halogen or a sulfonic acid reactive derivative, in at least εtoichiometric amounts, in the presence of at least two moles of a base selected from alkali or alkaline-earth metal carbonates per mole of substrate, at a temperature of 25-150°C, to give the compound of formula (II); step c) is the oxidation of the compound of formula (II) with a suitable oxidizing agent for aliphatic amines, in water or in a diphasic system consisting of water and an organic solvent, resistant to such conditions, at temperatures from 0 to 100°C, to give a mixture of oxidized products which is subjected directly to step d), consisting of the hydrolysis, either in aqueous acidic medium at pH lower than 2 , or in an aqueous basic medium at pH higher than 12, at temperatures of 110-200°C, to give the compound of formula (III). Compound (III) is the starting material for the synthesis of polydentated derivatives capable of complexing different metals, some of which find use in the biomedical field, such as gadolinium complexes of said derivatives, which are used in the medical diagnostic field as contrast agents for the resonance technique (Magnetic Resonance Imaging, MRI ) . At present, two contrast media are commercially available, namely Dotarem( R ' (gadolinium complex of 1 ,4 , 7 ,10-tetraazacyclododecane-l , 4 , 7 , 10-tetraacetic acid meglumine salt), and ProHance( R ) [Gadoteridol, gadolinium complex of 10- ( 2-hydroxypropyl )-l , 4 , 7 , 10- tetraazacyclododecane-1 , 4 , 7-triacetic acid]; on the other hand, Gadobutrol is a contrast medium at present under development (gadolinium complex of [10-[2,3- dihydroxy-l-(hydroxymethyl )propyl]-l ,4,7, 10-tetraaza- cyclododecane-1 , , 7-triacetic acid] ) The possible procedures for the conversion of compound (II) to 1 ,4 ,7 ,10-tetraazacyclododecane (III) were described in WO 96/28432 and in MI 96A 001257, and they are based on suitably optimized hydrolytic or oxidizing conditions for the preparation of compound (III). These novel synthetic routes for the preparation of compound (III) are an important improvement of the conventional synthesis by Richman-Atkins (J. Am. Chem. Soc, 1974, 96, 2268) based on the use of tosyl derivatives, in that they provide simpler, more economic and environmentally safer industrial processes. However, except for Gd-DOTA, obtainable from 1 , 4 , 7 , 10-tetraazacyclododecane (III) by alkylation of the four nitrogen atoms and subsequent co plexation of the intermediate 1 , 4 , 7 , 10-tetraazacyclododecane- 1 , 4 , 7 , 10-tetraacetιc acid, macrocycle (III) cannot, as a rule, be used directly for the preparation of gadolinium complexes wherein the macrocycle nitrogen atoms are variously substituted. In these cases it is, m fact, necessary to make use of the selective protection of the nitrogen atoms to direct the subsequent functionalization of the macrocycle towards the desired positions. For example, the key intermediate of the synthesis of Gadoteridol and Gadobutrol is 1,4,7,10- tetraazacyclododecane-1 , 4 , 7-trιacetιc acid (D03A), preparabile starting from (III) (see for ex.: Tweedle et al., Inorg. Chem., 1265, 1991) through the tricyclic intermediate of formula (IV) according to the scheme reported in the following When such disubstituted derivatives of compound (III) are desired, as, for example, 1,4,7,10- tetraazacyclododecane-1 , 7-dιacetιc acid (1,7-D02A), the synthetic route starting from macrocycle (III) is extremely complex, as described, for example, by Sherry in a paper (J. Chem. Soc. Chem. Commun., 1995, 185) and it is based on a series of selective protections, which make use of reactives rather unsuitable for possible industrial applications, mainly due to economic and environmental reasons; (III) l,7-DO2A or as described by the same author in WO 93/12097, which, however, involves the use of cyanides. (in) 1,7-D02A What stated above clearly shows that any direct synthetic routes for the preparation of D03A or 1,7-D02A or, in general, of 1 , 4 , 7 , 10-tetraazacyclododecane (III) trisubstituted or disubstituted derivatives would, on one hand, be a remarkable improvement in the processes for the synthesis of Gadoteridol and Gadobutrol and, on the other hand, open a way to the preparation of possible novel complexes for MRI , which are, at present, available with difficulty due to the above cited synthetic problems. It has now surprisingly been found, and this is the object of the present invention, that the novel compound (I), synthesized starting from compound (II), according to the following Scheme 1: Scheme 1 (II) (I) wherein compound (II) is reacted with piperazine at controlled pH to give compound (I), is a useful intermediate for the preparation of 1 ,4 , 7 ,10-tetraazacyclodode- cane-1 ,4 , 7 ,10-tetraacetic acid derivatives. Contrary to what described in literature by Weisman (Tetrahedron Letters, 1980, 21, 335) and by Kolinski (Tetrahedron Letters, 1981, 22, 2217), compound (II) is highly reactive also in hydrolytic conditions. The conversion of (II) to (I) involves heating (II) in aqueous solution at temperatures from 80 to 100°C, at pH slightly acidic, neutral or slightly basic (pH 5-9), and in the presence of piperazine, added in amounts ranging from 2 to 20 mol/mol of (II). At the end of the reaction, the mixture is alkalinized, concentrated to dryness and extracted with toluene. The toluene solution is partially concentrated and cooled to separate piperazine, which is filtered and can be purified and recycled. Toluene mother liquors are further concentrated, to obtain (I), which can be recrystallized, for example, from toluene. Alternatively, at the end of the reaction, the inorganic anions can be removed by ion-exchange. Eluates are suitably concentrated and added with orthophosphoric acid to pH 6.5, thereby precipitating piperazine . HgP0 4 salt. After filtration, the residual phosphate ions are removed from mother liquors by a second ion-exchange, eluates are concentrated to a residue which is recrystallized from toluene, to obtain (I) at high purity. Compound (I) showed an extremely interesting reactivity, in particular it can be used for the preparation of 1 , 4 , 7 , 10-tetraazacyclododecane (III) variously substituted derivatives, avoiding the step of formation of (III) itself, which iε, on the contrary, mandatory when following the procedure at present available in literature. Compound (I) is a lactam with a 6 atoms cycle and should therefore be remarkably stable in hydrolysis conditions . Surprisingly, compound (I) is converted by basic hydrolysis, in. not quite drastic conditions, to 1 ,4 , 7 , 10-tetraazacyclododecane-l-acetic acid of formula (V), i.e. to a N-monofunctionalized derivative of compound (III), according to the following Scheme 2: Scheme 2 (I) (V) Compound ( I ) can be hydrolyεed in aqueouε basic solution by heating to temperatures from 60 to 100°C, at pH higher than 12. It is a further object of the present invention the process for the preparation of the compounds of formula (VII) starting from compound (V), obtained according to the process represented in Scheme 2, by alkylation, according to known methods, with an excess of an R-CH(X)-C0Y alkylating agent of formula (VI), optionally followed by hydrolysis of the ester groups present, represented in the following Scheme 3: Scheme 3 (I) (V) in which R is a hydrogen atom, a straight, branched or cyclic C 1~ C 6 a lkyl group, unsubstituted or substituted with 1 to 10 oxygen atoms, or a alkyl group, optionally interrupted by a phenylene, phenyloxy or phenylenedioxy , in its turn substituted with a straight or branched c ι~ c 6 alkyl group, unsubstituted or substituted with 1 to 7 hydroxy groups or with 1 to 3 C^-C-y groups; the aromatic group can be unsubstituted or substituted with alkoxy groups or halogens, carboxy, carbamoyl, alkoxycarbonyl , εulfa oyl, hydroxyalkyl, amino, acylamino, acyl, hydroxyacyl groups; X is a halogen or a sulfonic acid reactive residue, and Y is a -OH or -OR., group, wherein R- is a straight or branched -C 4 alkyl group, when Y is the same as -OR^ the ester groups are also hydrolysed, according to known methods, to obtain the compoundε of formula ( VII ) . The alkylating agents (VI) corresponding to compound R-CH(X)-COOH of formula (VIII), and in which X is bromine or chlorine, are preferred, particularly preferred being the alkylating agents of formula (VIII), in which R is a hydrogen atom, corresponding to compound of formula (Villa), XCH2COOH, in which X is bromine or chlorine . In the other cases, the alkylating agent (VI) can be selected from the compounds which either are already commercially available or can be prepared aε already described in literature (see for example WO 93/24469 or EP 325762), or by using, for example, known methods for the preparation of suitable precursors (e.g., for acid chlorides α-halogen derivatives see: Harpp et al., J. Org. Chem., 40, 3420, 1975), and subsequent transformation into the desired product. Preferably, the R group can be selected from the group consisting of: H or a straight or branched alkyl group, such as a methyl, ethyl, propyl, isopropyl, butyl, isobutyl group, in itε turn substituted with hydroxy groups or interrupted by oxygen atoms, as defined above. When an aromatic group is present in R, particularly preferred are: phenyl, benzyl, phenylmethoxymethyl groups. Particularly preferred are 3-(phenylmethoxy )pro- panoic acid reactive derivatives, such as 2-bromo-3- (phenylmethoxy )propanoic acid, the preparation of which is described in Grossman et al., Chem. Ber . , 91, 538, 1958, and 2-chloro-3- (phenylmethoxy )propanoic acid (CAS RN 124628-32-6), prepared analogously to the brominated derivative . On the other hand, the R^ group is preferably selected from methyl, ethyl, isopropyl, butyl, tert- butyl. The reactive group X can be selected, by way of example, from the group consisting of halogens (Cl, Br , I), or it is the mesylate group (MeSθ2θ ~ ), the benzenesulfonyloxy group (PhS0 2 0 ~ ), the nitrobenzenesulfonyloxy group (p-N0 2 PhSθ2θ ~ ) , the tosylate group (TsO ~ ), the triflate group (CFgSOg-). Particularly preferred are the compounds in which X is a halogen, more particularly a bromide or a chloride. The alkylation of compound (V), when Y is a hydroxyl, can be conveniently performed with reactive derivatives of secondary carboxylic acid, such as 2- bromopropionic acid, in aqueous alkali solution, at temperatures from 25 to 55°C. Particularly preferred are the alkylating agents of formula (VI), in which Y is a hydroxyl, corresponding to bromoacetic acid (commercially available product), 2- bromopropionic acid (commercially available product), 2- bro obutyric acid (commercially available product). When the alkylation reaction is carried out with an ester derivative of compound (VIII), the reaction solvent can suitably be selected from dipolar aprotic solvents, in particular from dimethylforma ide (DMF), di ethylacetamide (DMA), dimethylsulfoxide (DMSO), acetonitrile (CHgCN) and N-methylpyrrolidone , and the reaction is carried out in the presence of an organic base, preferably an aliphatic tertiary a ine selected from triethylamine (TEA), diiεopropylethylamine (DIPEA) and tributylamine . In this case, it can be convenient to transform also the acid group present in compound (V) into the ester group -OR., , to promote the alkylation reaction, depending on the reactivity of the alkylating agent itself. The reaction temperature can range, in this case, from 0 to 80°C, in any case depending on the reactivity of the selected alkylating agent. In this case, the alkylation reaction will be followed by a basic hydrolysis of the resulting trieεter, in conventional conditions, to obtain the desired compound of formula (VII). The process of the present invention for the preparation of derivatives (VII), bearing three substituents corresponding to formula -CH(R)COOH and one -CH 2 COOH substituent, is a brilliant, economically advantageous solution to the problem of differentiating the four nitrogen equivalent sites. Compounds of this type, useful for the preparation of contrast agents for diagnosis by nuclear magnetic resonance, have already been described, for example, in EP 325,762. The preparation of these compounds according to the process of the present invention is economically more convenient and industrially more efficient, aε it involves no use of compound (III) as starting substrate, which has a prohibitive cost (ranging from 67,000 to 98,000 Italian Lire per gram, depending on the manufacturer). By way of example of the huge potentialities given by this synthetic route, the synthesis of the novel compound, a , a f ,α' ' -tris(methyl )-l , 4 , 7 , 10-tetraazacyclo- dodecane-1 , 4 , 7 ,10-tetraacetic acid, iε reported in Experimental section: as well as that of α, α ' , α ' ' -tris[ (phenylmethoxy )methyl )- 1,4,7, 10-tetraazacyclododecane-l ,4,7, 10-tetraacetic acid which, by catalytic hydrogenation, as described in example 6 of the cited Patent, yields α,α',α"- triε(hydroxymethyl )-l ,4,7 ,10-tetraazacyclododecane- 1 ,4, 7 , 10-tetraacetic acid. Particularly preferred iε the proceεs according to scheme 4 for the preparation of 1,4,7,10- tetraazacyclododecane-1,4, 7 , 10-tetraacetic acid by alkylation with the alkylating agent (Villa), so as to directly synthesize DOTA starting from compound (I), according to the following scheme: Scheme 4 rø (V) in which X, Y have the meanings defined above. Compound (V) can, in fact, be easily alkylated with chloro- or bromoacetic acid, in aqueouε alkaline solution, at temperatures from 25 to 55°C. It is a further object of the invention an alternative process for the preparation of the compounds (VII), as represented in Scheme 5, starting from compound ( I ) Scheme 5 0) (IX) (X) (VII) through a selective monoalkylation reaction, according to known methods, at controlled pH, with the alkylating agent (VI) as defined above, to give compound (X), which can subsequently be alkylated with the alkylating agent (VI) to give compounds (VII). It is a further object of the present invention the process for the preparation of the compounds of formula (XII), as represented in Scheme 6, starting from compound (X), obtained according to the process represented in Scheme 5 Scheme 6 (X) (xπ) in which compound (X) is alkylated, according to known methods, with the alkylating agent (XI) in which R' , independently of R, has the same meanings as R. An example of the possibilities given by this novel proceεs is the preparation of αl,α7- biεt (phenyl ethoxy)methyl )-α4-methyl-l ,4,7, 10-tetraaza- cyclododecane-1 , 4 , 7 , 10-tetraacetic acid which, after catalytic hydrogenation, giveε the debenzylated analogue . Particularly preferred is the preparation of 1,7- D02A, according to scheme 5, through formation of the novel compound 12-oxo-l ,4 , 7 ,10-tetraazabicyclo[8.2.2]te- tradecane-4-acetic acid of formula (XIII), which is subjected to basic hydrolysis to give 1,7-D02A, i.e. 1 ,4 ,7 , 10-tetraazacyclododecane-l , 7-diacetic acid, as represented in Scheme 7 Scheme 7 ) (XIII) Compound (I) can be selectively carboxymethylated in aqueous solution, for example with bromoacetic acid (1 ol) at pH 12.5 and at temperatures from 15 to 25°C, or in dimethylacetamide in the presence of sodium carbonate (1 mol) or of diisopropylethylamine , or in ethanol and sodium hydroxide: the resulting intermediate (XIII) can be transformed into 1,7-D02A by basic hydrolysis at pH higher than 13 and at temperatures from 80 to 100°C. 1,7-D02A can, in its turn, be tranεfor ed into DOTA by reaction with the alkylating agent of formula (Villa) . 1,7-D02A DOTA The conditions used, for example, for the alkylating agent (Villa) comprise carrying out the reaction in aqueous solution, at pH 10-12 and at temperatures from 25 to 50°C. According to the procesε repreεented in Scheme 6, when the alkylating agent (VI) correεponds to the alkylating agent (VIII), compounds of formula (XIV) can be prepared, aε represented in Scheme 8: Scheme 8 ) 1,7-DOZA in which R, X and Y have the meanings defined above. When R in the alkylating agent (VI) is the phenylmethoxymethyl group, it is poεεible to prepare, by uεing the alkylating agents preferred for this type of subεtituent defined above, αl , α7-biε[ (phenylmethoxy)- methyl]-! ,4,7 , 10-tetraazacyclododecane-l ,4,7, 10-tetraacetic acid, already described in EP 325,762, and its debenzylated analogue upon catalytic hydrogenation. Again starting f rom compound ( I ) , it is also poεsible to perform the complete dif ferentiation of the four nitrogen atoms of tetraazacyclododecane , to give compounds ( XVI I I ) , according to the following Scheme 9 : Scheme 9 o (I) o χ ) (XV) OH " (XVI) (XVIII) in which X, Y have the meanings defined above and R, R' , R 11 have independently the same meanings. An example of said synthetic approach is the preparation of αl-[ (phenylmethoxy)methyl]-α4-methyl-α7- ethy1-1, 4, 7 , 10-tetraazacyclododecane-l ,4,7 , 10-tetraacetic acid and of itε debenzylated analogue, after catalytic hydrogenation. Particularly preferred iε the process according to scheme 9, in which R and R' have the same meanings, repreεented in the following Scheme 10 (i) (XV) (XX) (XXI) Also preferred iε the process according to εche e 10, when R, R' and R 1 ' are at the εame time a hydrogen atom, for the preparation of D03A, obtained by alkylation of compound (I) with the alkylating agent (Villa), XCH 2 COOH, in which X has the meaningε defined above, to give the novel compound, 11-oxo-l , 4 , 7 , 10- tetraazacyclododecane-4,7-diacetic acid of formula (X), which is transformed into D03A, 1,4,7,10- tetraazacyclododecane-1 ,4 , 7-triacetic acid, by basic hydrolyεiε, according to εcheme 11 Scheme 11 (I) (X) The reεulting D03A can be uεed aε εtarting product for the synthesis of Gadoteridol or of Gadobutrol, as already described in the above cited references. The compound (I) can be directly transformed into intermediate (X) by reaction with 2 moles of the alkylating agent (Villa), in aqueous solution, at pH 13 and at a temperature from 25 to 45 °C. Compound (X) can be converted to D03A by basic hydrolyεis at pH higher than 13 and at temperatures from 80 to 100°C. A further aspect of the procesε according to scheme 9, in which the alkylating agent (XVII) corresponds to that of formula (VIII), relates to the posεibility to εelectively obtain 1 , 4 , 7 , 10-tetraazacyclododecane-l , 4- diacetic acid derivatives, of formula (XXII), aε repreεented in the following Scheme 12. Scheme 12 (I) (IX) (XV) R R HOOC A W ΓΛ N H "COOH HOOC A / / "" -COOH OH " ► N N HOO N COOH (Villa) HOOX ■ γ „NU N ^ COOH R' R' (XVI) (XXII) An example of this synthet ic εcheme is the preparation of α4-methyl-α7 -methyl-l , 4 , 7 , 10-t etraaza- cyclododecane-1 , 4 , 7 , 10-tetraacet ic ac id (I) Alεo preferred iε the process, according to scheme 9, for the preparation of the compounds of formula (XXIII), in which R and R 1 have the same meaning and the alkylating agent (XVII) corresponds to that of formula (Villa), represented in the following Scheme 13. Scheme 13 () (XIX) (XX) (xxm) For example, according to the process of Scheme 13, an alternative synthesis of αl , σ4-bis[ (phenylmethoxy ) methy1]-1 ,4,7 ,10-tetraazacyclododecane-l , 4 , 7 , 10- tetraacetic acid, already described in EP 325762, iε poεεible, aε well aε that of itε debenzylated analogue, which can be prepared by catalytic hydrogenation. In subεtance, compound (I) is a synton useful to obtain 1 ,4 , 7 , 10-tetraazacyclododecane (TAZA) derivatives both mono-, and di- , tri- or tetrasubstituted (symmetrically or asymmetrically), without the troublesome use of the macrocycle itself as starting product. In the following, some examples of preparation according to the method of the present invention are reported. Experimental section Example 1 Synthesis of 1 , 4 , 7 , 10-tetraazabicyclo[8.2.2] tetra- dodecan-2-one (I) 500 g (2.54 mol) of 2α , 4α , 6α8α-decahydro- tetraazacyclopent [fg]acenaphthylene , prepared aε described in MI96A001257, are disεolved in 4 L of water. 3.9 kg (20.0 mol) of piperazine hexahydrate are added and pH is adjusted to 6 with cone. HC1 (about 2.86 kg). The solution is refluxed for 24 h, then cooled to room temperature, percolated on 22L of anionic exchanger resin Relite 3AS/FB (Resindion) and the column is washed with deionized water. The fractions containing the product are concentrated at reduced pressure to a volume of 8 L, then cooled to 5°C and slowly added with 2.34 kg (20.0 mol) of 84% w/w orthophosphoric acid to pH 6.5. After lh, the precipitated εolid (piperazine . HgPO ) is filtered and washed on the filter with water precooled at 5°C. The washings are added to the filtrate, which iε percolated on 2 L of anionic exchanger resin Relite 3AS/FB (Resindion), then concentrated to dryness at reduced pressure and redissolved in hot toluene. Inεolubleε are filtered off. The toluene solution is concentrated to 1 kg weight and cooled to 5°C. After 12h the crystallized εolid iε filtered and washed on the filter with some cold toluene. The resulting product, after drying at 50°C under vacuum, weighs 388 g. Yield: 72% Humid piperazine . H3PO 4 is dried at 50 °C under vacuum to obtain 3.8 kg of piperazine . H 3 P0 4 (water content: 9%). Recovery yield: 95% The 1 H-NMR, 13 C-NMR, IR and MS spectra are consiεtent with the indicated structure. Example 2 Synthesis of 1 , 4 , 7 ,10-tetraazacyclododecane-l- acetic acid (V) 10 g of l,4,7,10-tetraazabicyclo[8.2.2]tetrado- decan-2-one (0.047 mol), prepared according to Example 1, are disεolved in 30 g of 10% w/w NaOH. The solution is heated at 100°C for 8h, then cooled to room temperature, diluted with 70 g of water and percolated on a column containing 150 ml of cationic exchanger reεin Duolite C20MB (Rohm & Haas), eluting with 2N ammonia, to collect about 1L of eluate, which is concentrated to dryness at reduced pressure and at a temperature of 50°C. The crude product is redisεolved in 30 g of water and treated with 11 g of cone, εulfuric acid. The εolution is cooled to 5°C and added with 60 g of acetone. After 6h the mixture iε filtered and the solid is washed with an acetone / water = 2 / 1 w/w mixture. After drying at 50°C under vacuum, 18.2 g of 1 , 4 , 7 , 10-tetraazacyclododecane-l-acetic aci .2H2S0 4 are obtained . Yield: 90.8% The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 3 Syntheεis of 1 , 4 , 7 ,10-tetraazacyclododecane- 1,4,7 , 10-tetraacetic acid (DOTA) 10 g of 1,4,7 ,10-tetraazabicyclo[8.2.2]tetradode- can-2-one (0.047 mol), prepared according to Example 1, are dissolved in 30 g of 10% w/w NaOH. The solution iε heated at 100°C for 8h, then cooled to room temperature, diluted with 30 g of water and εlowly added with a εolution prepared diεεolving 22.68 g (0.165 mol) of bromoacetic acid in 40 ml of water. The mixture iε reacted at 45°C for 5h, keeping pH at 10.5-11 by addition of 2N NaOH, then cooled to room temperature and acidified with cone. HC1 to pH 2. After lh, the precipitated εolid iε filtered and waεhed on the filter with deionized water. The crude product is redisεolved in 400 ml of deionized water. The reεulting εolution is percolated on a column containing 400 ml of polyvinylpyridine resin (PVP), eluting at length with water. The useful fractions are combined and concentrated to dryneεs under vacuum, then dried in a static drier at 50°C under vacuum to obtain 17.7 g of DOTA. Yield: 93% The iH-NMR, 13 C-NMR, IR and MS spectra are conεiεtent with the indicated structure. Example 4 Syntheεis of α ,α α ' ' -triε(methyl )-l , 4 , 7 ,10-tetra- azacyclododecane-1 ,4,7 , 10-tetraacetic acid 16 g (0.075 mol) of 1 , 4 , 7 , 10-tetraazabi- cyclo[8.2.2]tetradodecan-2-one , prepared according to Example 1, are diεsolved in 50 g of 10% w/w NaOH. The solution is heated at 100°C for 8h, then cooled to room temperature, diluted with 45 g of water and slowly added with a εolution prepared dissolving 45.89 g (0.300 mol) of 2-bromopropionic acid in 40 ml of water. The mixture iε reacted at 45°C for 12h, keeping pH at 10.5-11 by addition of 2N NaOH, then cooled to room temperature and acidified with cone. HC1 to pH 2. After Ih, the precipitated solid is filtered and washed on the filter with deionized water. The crude product is redisεolved in 600 ml of polyvinylpyridine reεin (PVP), eluting at length with water. The useful fractions are combined and concentrated to dryness under vacuum, then dried in a static drier at 50°C under vacuum to obtain 29.2 g of the desired product. Yield: 87% The 1 H-NMR, 13 C-NMR, IR and MS spectra are consiεtent with the indicated εtructure . Example 5 Syntheεiε of α , α α '' -tris[ (phenylmethoxy )methyl]- 1,4,7 ,10-tetraazacyclododecane-l ,4, 7, 10-tetraacetic acid According to the procedure deεcribed in example 4, uεing 1 , 4 , 7 , 10-tetracyclododecane-l-acetic acid (prepared as deεcribed in example 2) and 2-bromo-3- ( phenylmethoxy )propanoic acid methyl ester or 2- trifluoromethaneεulfonate-2- (phenylmethoxy )-propanoic acid methyl eεter in DMF and in the preεence of triethylamine . The methyl ester iε hydrolyεed to obtain the deεired product. The 1 H-NMR, 13 C-NMR, IR and MS εpectra are conεiεtent with the indicated εtructure. Example 6 Syntheεiε of α , α α ' ' -triε(hydroxymethyl )-l , 4 , 7 , 10- tetraazacyclododecane-1 ,4,7 , 10-tetraacetic acid The product obtained in Example 5 iε εubjected to catalytic hydrogenation in the preεence of 5% Pd-on- charcoal, to obtain, after absorption of the necessary amount of hydrogen, the desired product. The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 7 Synthesiε of 12-oxo-l , 4 , 7 ,10-tetraazabi- cyclo[8.2.2]tetradecane-4-acetic acid (XIII) 50 g (0.235 mol) of 1 , 4 , 7 , 10-tetraazabi- cyclo[8.2.2]tetradodecan-2-one , prepared according to Example 1, are disεolved in 500 ml of deionized water. 36 g (0.259 mol) of bromoacetic acid are added, pH is adjusted to 12.5 by addition of IN NaOH and the mixture is reacted at room temperature for 12h, keeping pH at 12.5 through gradual additions of IN NaOH. The mixture is then cooled and percolated on a column containing 1L of cationic exchanger resin Duolite C20MB (Rohm & Haas). The column is repeatedly washed with deionized water, then eluted with 2N ammonia. The fractions containing the product are combined and concentrated at reduced pressure to a solid residue, which is redissolved in 500 ml of deionized water. The resulting solution iε percolated on a column containing 5L of resin Amberlite XAD 1600, and eluted with water. The fractions containing the pure product are combined and concentrated to dryness at reduced pressure, then dried in a static drier at 50°C under vacuum, to obtain 49.5 g (0.183 mol) of 12-oxo-l , 4 , 7 , 10-tetraazabicyclo- [8.2.2] tetradecane-4-acetic acid. Yield: 78% The iH-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 8 Synthesiε of αl , α7-bis[ (phenylmethoxy )methyl )-α4- methyl-1 ,4,7, 10-tetraazacyclododecane-l ,4,7, 10-tetraacetic acid A) Preparation of 12-oxo-l , 4 , 7 , 10-tetraazabi- cyclo[8.2.2]tetradecane-α4-methyl-4-acetic acid The product is prepared analogously to the procedure of Example 7 , using the 2-bromopropionic acid instead of bromoacetic acid, to obtain the desired product . The iH-NMR, 13 C-NMR, IR and MS spectra are consiεtent with the indicated εtructure . B) α4-Methyl-l ,4,7, 10-tetraazacyclododecane-l , 7-diace- tic acid According to the procedure deεcribed in example 2 the deεired product is obtained by basic hydrolysiε of the product obtained at the preceding εtep. The l-H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. C) αl,α4-bis[ (phenylmethoxy ) ethyl )-α7-methyl-l ,4,7 ,- 10-tetraazacyclododecane-l ,4,7 , 10-tetraacetic acid According to the procedure described in example 5, the product from the preceding step is alkylated with 2- bromo-3- ( phenylmethoxy )propanoic acid methyl ester or with 2-trifluoromethane ulfonate-3- (phenylmethoxy )propa- noic acid methyl ester. The dieεter iε hydrolyεed to obtain the desired product. The 1 H-NMR, 13 C-NMR, IR and MS spectra are consiεtent with the indicated εtructure . Example 9 Synthesis of αl , α7-bis (hydroxymethyl )-α4-methyl- 1,4,7, 10-tetraazacyclododecane-l ,4,7, 10-tetraacetic acid According to the procedure described in Example 6, the product described in example 8 is subjected to catalytic hydrogenation, to obtain the desired product. The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 10 Synthesiε of 1 , 4 , 7 , 10-tetraazacyclododecane-l , 7- diacetic acid (1,7-D02A) 20 g (0.074 mol) of 12-oxo-l , 4 , 7 , 10-tetraazabi- cyclo[8.2.2] tetradecane-4-acetic acid, prepared aε described in example 5, are disεolved in 88.8 g of 10% NaOH. The solution is refluxed overnight, then cooled and acidified to pH 12 by addition of cone. HC1. The resulting solution is percolated on a column containing 600 ml of anionic exchanger resin IRA420 (Rohm & Haas), which is washed repeatedly with water, then eluted with IN HC1. The fractions containing the product are combined and concentrated at reduced pressure to a volume of about 500 mL . The εolution is percolated on a column containing 2L of polyvinylpyridine resin (PVP), eluting with water, the fractions containing the desalted product are collected, combined and concentrated to dryness under vacuum. The crude solid iε recryεtallized from methanol / acetone = 8 / 2, finally dried in a εtatic drier at 50°C under vacuum. 18.5 g (0.064 mol) of 1 , 4 , 7 , 10-tetraazacyclododecane-l , 7- diacetic acid are obtained. Yield: 86% The 1 H-NMR, 13 C-NMR, IR and MS εpectra are consistent with the indicated structure. Example 11 Synthesis of 1 , 4 , 7 ,10-tetraazacyclododecane-l , 7- diacetic acid (1,7-D02A) 50 g (0.235 mol) of 1 , 4 , 7 , 10-tetraazabi- cyclo[8.2.2]tetradodecan-2-one , prepared according to Example 1, are dissolved in 500 ml of deionized water. 36 g (0.259 mol) of bromoacetic acid are added, pH is adjusted to 12.5 by addition of IN NaOH and the mixture iε reacted at room temperature for 12h, keeping pH at 12.5 through gradual additions of IN NaOH. pH iε adjuεted to 14 with NaOH in pellets and the solution iε refluxed overnight, then cooled to room temperature and diluted with 500 ml of water. The reεulting εolution iε percolated on a column containing IL of cationic exchanger resin C20MB (Rohm & Haas), washing repeatedly with water, then the product iε eluted with 2N ammonia. The uεeful fractions are combined and concentrated to dryneεε at reduced preεεure. The crude εolid iε redissolved in IL of deionized water and the resulting solution is percolated on a column containing 5L of resin A berlite XAD1600, eluting with deionized water. The uεeful fractions are concentrated at reduced pressure to a solid residue, which is finally recrystallized from methanol / acetone = 8 : 2, to obtain 54,9 g (0.190 mol) of 1,4,7,10- tetraazacyclododecane-1 , 7-diacetic acid . 5 Yield: 81% The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 12 Synthesiε of αl , α7-bis[ (phenylmethoxy )methyl ) ]- 0 1,4,7 ,10-tetraazacyclododecane-l,4 , 7 , 10-tetraacetic acid According to the procedure described in example 8C, 0 1,7-D02A acid is reacted with 2-bromo-3- ( phenylmethoxy )propanoic acid methyl ester or with 2- trifluoromethaneεulfonate-3- (phenylmethoxy )propanoic acid methyl ester. The diester is hydrolyεed to obtain the deεired product. 5 The iH-NMR, 13 C-NMR, IR and MS εpectra are consistent with the indicated structure. Example 13 Synthesiε of αl ,α7-biε(hydroxymethyl )-l ,4 , 7 , 10- tetraazacyclododecane-1 ,4,7, 10-tetraacetic acid According to the procedure deεcribed in example 6, the product described in example 12 is subjected to catalytic hydrogenation to obtain the deεired product. The iH-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 14 Synthesis of αl- [ (phenylmethoxy )methyl ) ]-α4-methyl- α7-ethyl-l ,4,7 , 10-tetraazacyclododecane-l , 4 , 7 , 10-tetraacetic acid A ) α4-Ethyl-α7-methyl-ll-oxo-l ,4,7 , 10-tetraazabicyclo- [8.2.2]tetradecane-4,7-diacetic acid 12-Oxo-l ,4,7 ,10-tetraazabicyclo[8.2.2]tetradecane- α4-methyl-4-acetic acid, prepared according to the procedure deεcribed in example 8A, iε reacted with 2- bromobutanoic acid according to the εame procedure. The ^-H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. B) αl-Methyl-α4-ethyl-l ,4,7 , 10-tetraazacyclododecane- 1 ,4, 7-triacetic acid α4-Ethyl-α7-methγl-ll-oxo-l ,4,7, 10-tetraazabi- cyclo[8.2.2]tetradecane-4,7-acetic acid, prepared in the above step, is hydrolysed according to the procedure deεcribed in example 2, to obtain the desired product. The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. C) αl-[ (Phenylmethoxy)methyl) ]-α4-methyl-α7-ethyl- 1,4,7 ,10-tetraazacyclododecane-l ,4,7, 10-tetraacetic acid According to the procedure described in example 8C, αl-methyl-α4-ethyl-l,4,7 , 10-tetraazacyclododecane-l ,4,7- triacetic acid is reacted with 2-bromo-3- (phenylmethoxy )propanoic acid methyl ester in DMF and in the preεence of triethylamine . The resulting eεter is hydrolyεed to obtain the deεired product. The ^-H-NMR, 13 C-NMR, IR and MS εpectra are consistent with the indicated structure. Example 15 Synthesis of αl-ethyl , α4-methyl , α7-( hydroxymethyl )- 1,4,7, 10-tetraazacyclododecane-l ,4,7, 10-tetraacetic acid According to the procedure described in Example 6, the product described in example 14 is subjected to catalytic hydrogenation to obtain the desired product. The iH-NMR, 13 C-NMR, IR and MS spectra are consiεtent with the indicated structure. Example 16 Synthesis of α4-ethyl ,α7-methyl-l ,4 , 7 , 10-tetraaza- cyclododecane-1 ,4,7 , 10-tetraacetic acid αl-Methyl-α4-ethyl-l ,4,7 , 10-tetraazacyclododecane- 1 ,4 , 7-triacetic acid, prepared according to the procedure deεcribed in example 14B, is reacted with bromoacetic acid, according to the procedure described in example 3, to obtain the desired product. The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 17 Synthesis of ll-oxo-1,4,7 ,10-tetradecane-4,7- diacetic acid (X) 50 g (0.235 mol) of 1 ,4 ,7 ,10-tetraazabi- cyclo[8.2.2]tetradodecan-2-one, prepared as described in example 1, are dissolved in 500 ml of deionized water. 78 g (0.564 mol) of bromoacetic acid are added, pH is adjusted to 13 by addition of IN NaOH and the mixture is reacted at room temperature for 12h, keeping pH at 13 through gradual additions of IN NaOH. 500 ml of water are added and the resulting solution is percolated on a column containing 2.5L of cationic exchanger resin Duolite C20MB (Rohm & Haas), washing repeatedly with water, then eluting with 2N ammonia. The useful fractions are concentrated at reduced pressure to a residue, which is redissolved in 300 ml of deionized water. The solution is percolated on a column containing 4L of resin Amberlite XAD1600, eluting subsequently with deionized water. The fractions containing the pure product are combined and concentrated to dryness at reduced pressure, finally dried in a static drier at 50 °C under vacuum, to obtain 60.2 g (0.183 mol) of the desired product. Yield: 78% The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated structure. Example 18 Synthesiε of 1 ,4 , 7 , 10-tetraazacyclododecane-l , 4, 7- triacetic acid (D03A) εodium salt 50 g (0.152 mol) of 11-oxo-l , 4 , 7 , 10-tetraaza- cyclododecane-4 , 7-diacetic acid, prepared aε deεcribed in example 8, are dissolved in 240 g of 10% NaOH. The solution is refluxed overnight, then cooled and pH is adjusted to 12 by addition of cone. HCl. The resulting solution contains 52,6 g (0.152 mol) of 1,4,7,10- tetraazacyclododecane-1 , 4 , 7-triacetic acid, as trisodium εalt, which can directly be used for the synthesis of tetraacetic acid ligands differentiated at the 1- position. Example 19 Preparation of Gadoteridol Gadoteridol The solution of 1 , 4 , 7 ,10-tetraazacyclododecane- 1,4 , 7-triacetic acid sodium salt is directly reacted, as described in EP 292689, with propylene oxide to give the alkylated product, which can easily be complexed with gadolinium to give the final Gadoteridol. Example 20 Preparation of Gadobutrol The solution of 1 , 4 , 7 , 10-tetraazacyclododecane- 1 ,4 , 7-triacetic acid sodium salt is directly reacted, as described in EP 448191, with 4 , 4-dimethyl-3 , 5 ,8- trioxabicyclo[5.1.0]octane to give the alkylated product, which can. easily be complexed with gadolinium to give the final Gadobutrol. Example 21 Synthesis of 1 , 4 , 7 , 10-tetraazacyclododecane-l , 4 , 7- triacetic acid (D03A) 50 g (0.235 mol) of 1 , 4 , 7 , 10-tetraazabi- cyclo[8.2.2]tetradodecan-2-one, prepared as described in example 1, are dissolved in 500 ml of deionized water. 78 g (0.5646 mol) of bromoacetic acid are added, pH is adjusted to 13 by addition of IN NaOH and the mixture is reacted at room temperature for 12h, keeping pH at 13 through gradual additions of IN NaOH. pH is adjusted to 14 with NaOH in pellets and the solution is refluxed overnight, then diluted with 500 ml of deionized water and percolated on a column containing 3L of cationic exchanger reεin Duolite C20MB (Rohm & Haaε). The column iε repeatedly washed with water, then eluted with 2N ammonia. The fractions containing the product are combined and concentrated at reduced pressure to a residue, which is redissolved in 300 ml of water. The solution is acidified with sulfuric acid to pH 2, cooled and gradually added with acetone (600 mL ) . After 3h cryεtallization, the solid is filtered, washed with a water / acetone = 1 : 2 mixture, then dried in a static drier at 50°C under vacuum to obtain 78 g (0.176 mol) of D03A, salified as sulfate. Yield: 75% The 1 H-NMR, 13 C-NMR, IR and MS εpectra are consistent with the indicated structure. Example 22 Synthesis of α4 , α7-bis[ (phenylmethoxy )methyl]- 1,4,7, 10-tetraazacyclododecane-l ,4, 7, 10-tetraacetic acid A) αl,α4-bιs[ ( Phenylmethoxy ) ethyl ]-l , 4 , 7 , 10-tetraazacyclododecane-1 ,4 , 7-trιacetιc acid 1,4,7 ,10-tetraazabιcyclo[8.2.2] tetradodecan-2-one lε reacted with 2-bromo-3-(phenylmethoxy )propanoιc acid methyl eεter or with 2-trιfluoromethanesulfonate-3- ( phenylmethoxy )propanoιc acid methyl ester in DMF, m the presence of triethylamine . The ester is hydrolysed while opening the lactam, to obtain the deεired product. The 1 H-NMR, 13 C-NMR, IR and MS εpectra are conεistent with the indicated structure. B) αl,α4-bιs[ ( Phenylmethoxy )methyl ] -1 ,4,7 , 10-tetraaza- cyclododecane-1 ,4,7, 10-tetraacetic acid α4,α7-bιs[ (phenylmethoxy ) ethyl]-! , 4 , 7 , 10-tetraaza- cyclododecane-1 ,4 , 7-triacetic acid prepared in the above step is reacted with bromoacetic acid, according to the procedure described in example 3, to obtain the desired product . The 1 H-NMR / 13 C-NMR, IR and MS spectra are consiεtent with the indicated structure. Example 23 Synthesiε of αl , α4-biε (hydroxymethyl )-l , 4 , 7 , 10- tetraazacyclododecane-1 ,4,7, 10-tetraacetic acid The product described in example 22 is subjected to catalytic hydrogenation, according to the procedure described in example 6, to obtain the desired product. The 1 H-NMR, 13 C-NMR, IR and MS spectra are conεiεtent with the indicated εtructure. Example 24 Synthesis of 1 ,4 , 7 ,10-tetraazabicyclo[8.2.2]- tetradodecan-2-one (III) with piperazine in different pH, temperature and stoichiometry conditions The procedure described in example 2 is followed, in the conditions reported in the following table: mol piperazine/mol ( I ) pH T (°C) t (h) % Yield 2 5 100 48 35 2 6 100 48 48 2 7 100 48 45 4 6.5 100 24 55 6 6 80 48 62 6 9 100 24 52 7 7 80 48 55 7 8 100 12 40 8 8.5 100 48 48 8 9 100 24 42 10 7 100 18 56 10 6 100 12 65 30 5 100 24 55 Example 25 Synthesiε of 1 , 4 , 7 , 10-tetraazabicyclo[8.2.2]- tetradodecan-2-one (I) with piperazine, without recovery of piperazine .H 3 P0 4 50 g (0.254 mol) of 2α , 4α , 6α , 8α-decahγdro- tetraazacyclopent [fg]acenaphthylene , prepared aε described in MI96A001257, are disεolved in 500 ml of water. 173 g (2 mol) of piperazine are added thereto and pH iε adjusted to 6 with cone. HCl (about 286 g). The solution is refluxed for 24h, then cooled to room temperature, alkalinized to pH 12 with NaOH and concentrated to small volume in rotary evaporator at residual presεure. Methanol is added and the inorganic saltε are filtered off. The filtrate is concentrated to dryness at reduced pressure, then repeatedly extracted with hot toluene, and insolubleε are filtered off. The toluene extracts are combined, concentrated and cooled. After crystallization at 5"C overnight, the residue iε filtered and recrystallized from toluene, then dried in a static drier at 50°C under vacuum, to obtain 145 g of piperazine. Recovery yield: 83% The toluene mother liquors are further concentrated to small volume, then left to crystallize at 5 β C for 24 h, filtered and recrystallized from toluene, to obtain 31 g of (III) . Yield: 57% The 1 H-NMR, 13 C-NMR, IR and MS spectra are consistent with the indicated εtructure.

Claims: CLAIMS 1. 1,4,7 ,10-Tetraazabicyclo[8.2.2]tetradecan-2-one of formula ( I ) 2. A procesε for the preparation of compound 1,4,7,10- tetraazabicyclo[8.2.2]tetradecan-2-one (I), starting from compound 2α, 4α, 6α,8α-decahydro-tetraazacyclo- pent [fg]acenaphthylene of formula (II), according to the following Scheme: (II) (I) wherein compound (II) is reacted with piperazine to give compound (I), in which the pH of the reaction medium ranges from 5 to 9. 3. A process as claimed in claim 2, in which (II) is converted to (I) by heating (II) in aqueous solution at temperatures from 80 to 100°C, in the presence of piperazine added in amounts from 2 to 20 mol/mol of (II). 4. 1,4,7 ,10-Tetraazabicyclo[8.2.2]tetradecan-2-one, obtainable by the process according to claims 2 to 3. 5. A procesε for the preparation of 1,4,7,10- tetraazacyclododecane-1-acetic acid of formula (V) εtarting from compound (I) by baεic hydrolyεis, according to the following Scheme: (I) (V) 6. A process as claimed in claim 5, in which the hydrolysis reaction is carried out in basic aqueous solution by heating at temperatures from 60 to 100°C, and at pH higher than 12. 7. A procesε for the preparation of compoundε of formula (VII) starting from compound (V), obtainable according to claims 5-6, by alkylation, according to known methods, with an excess of an R-CH(X)-COY alkylating agent of formula (VI), optionally followed by hydrolysiε of the eεter groups present, represented by following Scheme: ( I ) (V) wherein : R is a hydrogen atom, a straight, branched or cyclic C^-Cg alkyl group, unsubεtituted or εubstituted with 1 to 10 oxygen atoms, or a C 1 -C 20 alkyl group, optionally interrupted by a phenylene, phenyloxy or phenylenedioxy , in its turn substituted with a straight or branched c ι _ 6 alkyl group, unsubεtituted or εubεtituted with 1 to 7 hydroxy groups or with 1 to 3 C^-Cy groups; the aromatic group can be unsubεtituted or εubεtituted with alkoxy groupε or halogenε, carboxy, carbamoyl , alkoxycarbonyl , εulfa oyl, hydroxyalkyl, amino, acylamino, acyl , hydroxyacyl groups; X is a halogen or a sulfonic acid reactive residue; Y is a -OH or -OR., group, wherein R 1 is a straight or branched C 1 -C 4 alkyl group; and when Y is the same as -0R 1 , the ester groups are alεo hydrolyεed to obtain the compounds (VII). 8. A process as claimed in claim 7, in which the alkylating agent (VI) correspondε to the compound of formula (VIII), R-CH( X )-COOH , in which X is bromine or chlorine . 9. A procesε aε claimed in claim 8, in which, in the alkylating agent (VIII), the R group is selected from the group consisting of: H, methyl, ethyl, propyl, isopropyl, butyl, isobutyl. 10. A procesε as claimed in claim 9, in which the alkylating agent (VIII) correεponds to compound (Villa), XCH 2 C00H. 11. A procesε according to claims 8-10, in which the alkylation reaction is carried out in aqueous alkali solution, at temperatures from 25 to 55°C. 12. A process according to claims 8-11, in which the alkylating agent (VI) is bromoacetic acid, chloroacetic acid, 2-bromopropionic acid or 2-bromobutyric acid. 13. A procesε as claimed in claim 12, for the preparation of a , a ',α '' -triε(methyl )-l , 4 , 7 , 10-tetraaza- cyclododecane-1 ,4 , 7 , 10-tetraacetic acid and of 1,4,7,10- tetraazacyclododecane-1 ,4,7 , 10-tetraacetic acid. 14. A process as claimed in claim 7, in which, in the alkylating agent (VI), the R group is selected from the group consiεting of phenyl, benzyl, phenylmethoxymethyl; the R^ group iε selected from the group conεiεting of: methyl, ethyl, iεopropyl, butyl, t-butyl; the reactive group X iε εelected from the group consisting of: mesylate, benzenesulfonyloxy, nitrobenzenesulfonyloxy , tosylate or triflate groups. 15. A process as claimed in claim 14, in which the reaction solvent is selected from the group consisting of: dimethylformamide , dimethylacetamide, dimethylsul- foxide, acetonitrile and N-methylpyrrolidone; in the presence of an organic base selected from the group consisting of: triethylamine (TEA), diisopro- pylethylamine and tributyla ine; the alkylation reaction is followed by basic hydrolysis of the resulting triester, in conventional conditions, to obtain the compounds of formula (VII). 16. A process as claimed in claim 15, in which the alkylating agent (VI) is selected from 2-bromo-3- (phenylmethoxy )propanoic acid methyl ester and 2- trifluoromethanesuIfonate-3- (phenylmethoxy )-propanoic acid methyl ester. 17. A process as claimed in claim 16, for the preparation of α, α ' , α '' -tris[ (phenylmethoxy)methyl]- 1,4,7 ,10-tetraazacyclododecane-l ,4,7 , 10-tetraacetic acid. 18. A process for the preparation of the compounds of formula (VII) starting from compound (I), through the monoalkylation reaction of the compound (I) with the alkylating agent (VI), to give the intermediate compound of formula (IX) and the subεequent hydrolyεis of the compound (IX), to give the intermediate compound of formula (X), which is alkylated again with the alkylating agent (VI), to give the compounds of formula (VII), as represented in the following Scheme: (I) (IX) (X) (VII) in which R, X and Y have the meanings defined in claim 7. 19. A procesε for the preparation of the compounds of formula (XII), in which compound (X), obtainable according to the process of claim 18, is alkylated with the alkylating agent (XI) aε repreεented in the following Scheme: (X) (XII) in which R' , independently of R, has the same meaningε as R defined in claim 7. 20. A process as claimed in claim 19, for the preparation of αl ,α7-bis[ (phenylmethoxy)methyl )-α4- methyl-1 ,4,7, 10-tetraazacyclododecane-l ,4,7 , 10-tetraacetic acid. 21. A process as claimed in claim 18, for the preparation of 1 ,4 ,7 ,10-tetraazacyclododecane-l ,7- diacetic acid (1,7-D02A) by alkylation of compound (I) with the alkylating agent (VIII) through the formation of the intermediate 12-oxo-l ,4 , 7 , 10-tetraazabi- cyclo[8.2.2]tetradecane-4-acetic acid (XIII), which by basic hydrolysis gives 1,7-D02A. 22. 12-Oxo-l ,4,7 , 10-tetraazabicyclo[8.2.2] tetradecane- 4-acetic acid of formula (XIII) as an intermediate in the proceεs as claimed in claim 21. 23. A process as claimed in claim 18, for the preparation of 1 , , 7 ,10-tetraazacyclododecane-l ,4 , 7 , 10- tetraacetic acid (DOTA), in which the alkylating agent (VI) corresponds to the alkylating agent (VIII) and the alkylation reaction is carried out in aqueous εolution at pH 10-12 and at temperatures from 25 to 50°C. 24. A process as claimed in claim 18, for the preparation of compounds of formula (XIV), in which the alkylating agent (VI) corresponds to the alkylating agent (Villa) in the first alkylation εtep, aε represented in the following Scheme: (I) 1,7-D02A in which R, X and Y have the meanings defined above in claim 7. 25. A procesε aε claimed in claim 24, for the preparation of αl , α7-bis[ (phenylmethoxy)methyl]- 1,4,7 ,10-tetraazacyclododecane-l ,4,7 , 10-tetraacetic acid. 26. A procesε for the preparation of the compounds of formula (XVIII), starting from compound (I) by alkylation reaction with the alkylating agents of formulae (VI), (XI) and (XVII), aε represented by the following Scheme: (XVI) pcvm) in which R , X and Y have the meanings def ined in claim 7 , R 1 has the meanings defined in claim 18 e R' X independently of R and R', has the same meanings. 27. A process as claimed in claim 26, for the preparation of αl-[ (phenylmethoxy )methyl]-α4-methyl-α7-ethy1-1 ,4,7 , 10- tetraazacyclododecane-1, 4 ,7 , 10-tetraacetic acid. 28. A proceεε aε claimed in claim 26, for the preparation of the compoundε of formula (XXI), according to the following Scheme: (I) (XV) (XX) (XXI) in which R, R' have the same meaning. 29. A process as claimed in claim 28, for the preparation of 1 ,4 , 7 ,10-tetraazacyclododecane-l , 4 , 7 ,10- triacetic acid (D03A), through the formation of the intermediate 11-oxo-l ,4,7, 10-tetraazacyclododecane-4 , 7- diacetic acid of formula (X), which is transformed into D03A by basic hydrolysiε, according to the following Scheme: (I) (X) 30. 11-Oxo-l ,4,7 ,10-tetraazacyclododecane-l ,4,7,10-dia- cetic acid of formula (X), aε an intermediate in the proceεε aε claimed in claim 29. 31. The use of 11-oxo-l , 4 , 7 , 10-tetraazacyclododecane- 1 ,4,7 ,10-diacetic acid of formula (X), for the preparation of D03A. 32. A process as claimed in claim 29, in which the intermediate (X) is formed from compound (I) by reaction with the alkylating agent (Villa), in aqueous solution at basic pH and at temperatures from 25 to 45"C. 33. A procesε aε claimed in claim 32, in which compound (X) can be converted to D03A by baεic hydrolyεiε at pH higher than 13 and at temperatures from 80 to 100°C. 34. A proceεs for the preparation of contrast agentε for magnetic reεonance tomography, uεing D03A prepared according to clai ε 29 to 33 as starting product. 35. A process for the preparation of Gadoteridol and Gadobutrol, using D03A prepared according to claims 29 to 33 as starting product. 36. The use of D03A obtainable according to claims 29 to 33, as εtarting product for the preparation of contraεt agents for magnetic resonance tomography. 37. The use of D03A prepared according to claims 29 to 33, as starting product for the preparation of Gadoteridol and Gadobutrol. 38. A procesε aε claimed in claim 28, for the preparation of the compoundε of formula (XXII), in which the alkylating agent (XVII) corresponds to that of formula (Villa) as represented in the following εcheme (I) (IX) (XV) (XVI) (XXI1) in which R, R', X and Y have the meaningε defined in claim 28. 39. A process as claimed in claim 38, for the preparation of α4-methyl-α7-methyl-l , 4, 7 , 10-tetraaza- cyclododecane-1 ,4,7, 10-tetraacetic acid . 40. A process as claimed in claim 28, for the preparation of the compounds of formula (XXIII), in which the alkylating agent (XVII) correspondε to that of formula (Villa) according to the following Scheme (I) (XIX) (XX) (XXIII) in which R and X have the meanings defined in claim 28. 41. A process as claimed in claim 40, for the preparation of αl ,α4-bis[ (phenylmethoxy)methyl]- 1,4,7, 10-tetraazacyclododecane-l ,4,7 , 10-tetraacetic acid.

Inventors: ARGESE MARIA, RIPA GIORGIO, ARGESE, MARIA, RIPA, GIORGIO
Applicants: ARGESE MARIA, BRACCO SPA, DIBRA SPA, RIPA GIORGIO, ARGESE, MARIA, BRACCO S.P.A., DIBRA S.P.A., RIPA, GIORGIO
Application year: 1998
Main classification: C07D487
IPC codes: A61K49, C07D257, C07D487