Amino acid derivatives and compositions containing the same for inhibiting active-oxygen

Abstract: A novel active oxygen-resisting agent is provided which has an excellent active oxygen resistance and which exhibits a good solubility in an oil solvent. The active oxygen-resisting agent contains as an active ingredient an amino acid derivative represented by the following formula (I) or its salt wherein Ar represents a 2-hydroxyphenyl group or a pyridyl group (one or more of hydrogen atoms bound to the aromatic rings of these groups may be substituted independently with a halogen atom, an alkyl group having from 1 to 6 carbon atoms, a hydroxyl group, a hydroxyalkyl group having from 1 to 6 carbon atoms, a nitro group, an alkoxyl group having from 1 to 6 carbon atoms or a carboxyl group), R 1 represents a side chain of an animo acid, X represents -O- or -NH-, R 2 represents an alkyl group having from 8 to 22 carbon atoms, and n represents 0 or 1.

Description: The present invention relates to novel amino acid derivatives. In particular, it relates to such derivatives having activity in inhibiting or resisting the action of active oxygen species. Examples of the derivatives, which may exhibit properties such as good solubility, may be included as active ingredients of compositions intended for inhibiting or resisting the action of active oxygen species. In recent years, various skin disorders and diseases attributable to active oxygen species have been reported. For example, it has been known that in the course of aging, canceration, pigmentation and inflammation of the skin by the sunlight, especially, by the ultraviolet light, active oxygen species are deeply involved as the cause thereof. Accordingly, if the action of the active oxygen species can be inhibited, it is expected that these disorders and diseases of the skin can be inhibited or even prevented. Enzymatic antioxidants including superoxide dismutase (SOD), non-enzymatic antioxidants such as ascorbic acid, tocopherol or glutathione, and antioxidants derived from vegetables, such as tannin have so far been known as substances that inhibit the action of active oxygen species. However, of these substances, the use of SOD is limited because it is costly and chemically unstable. Also the non-enzymatic antioxidants such as ascorbic acid, tocopherol or glutathione are unstable in many cases, and their effect on inhibiting the active oxygen species is unsatisfactory. Antioxidant derived from vegetables, such as tannin, may be easily hydrolyzed, and itself easily oxidized. Accordingly, these substances are problematic in terms of their stability in many cases. Further, in recent years, some investigations have been reported in which, the occurrence of the active oxygen species is controlled by catching a metal ion, which in vivo plays the role of a catalyst in the production of active oxygen species (for example, Free Radicals in Biology and Medicine, Oxford, Clarendon Press, p. 234, 1989). One example of a compound having the ability to catch a metal ion is, a disferrioxamine compound. Since this compound is, however, too strong in its ability to catch the metal ion, the balance of the metal ion in vivo is upset. Further, this compound is costly. Besides the disferrioxamine compound, metal ion chelating agents such as 2,2'-dipyridyl, 1,10-phenanthrolene and 2,2'-dipyridylamine have been studied. However, most of these compounds exhibit skin irritation. Amino acid derivatives which are stable, which show low skin irritation and which have an excellent active oxygen resistance are reported in WO 94/14755 (EP-A-0 629 606). Nevertheless since the derivatives have low solubility in a multi-purpose oil solvent such as liquid paraffin or an olive oil, the use thereof is limited. It is, therefore desirable that embodiments of the present invention provide a novel active oxygen-resisting agent which has an excellent active oxygen resistance and which exhibits a good solubility in an oil solvent. In addition to the prior art discussed above, US-A-4 382 872 discloses metallurgical extractant systems which, in some examples, include N-picolyl glycine, -2,6-dimethylheptyl ester or N-picolyl glycine, dodecyl ester or N-picolyl glycine, hexadecyl ester. FR-A-2.203.806 describes N-long chain acyl-acidic amino acid diesters useful as oil-soluble surfactants in the fields of cosmetics and perfumes. The present invention relates to an amino acid derivative represented by the following formula (I') or its salt wherein Ar represents a 2-hydroxyphenyl group or a pyridyl group (one or more of hydrogen atoms bound to the aromatic rings of these groups may be substituted independently with a halogen atom, an alkyl group having from 1 to 6 carbon atoms, a hydroxyl group, a hydroxyalkyl group having from 1 to 6 carbon atoms, a nitro group, an alkoxyl group having from 1 to 6 carbon atoms or a carboxyl group), R 1 represents a side chain of an amino acid, X represents -O- or -NH-, R 2 represents an alkyl group having from 8 to 22 carbon atoms, and n represents 0 or 1; with the proviso that when Ar-CH 2 - is an unsubstituted picolyl group, R 1 is hydrogen, n is 0 and X is O, then R 2 is not a -2,6 dimethylheptyl, a dodecyl, or a hexadecyl group. The amino acid derivative represented by the above-mentioned formula (I') is a novel compound undescribed in the literature. It can be included in an active oxygen resisting agent. In another aspect, the present invention provides an amino acid derivative represented by the following formula (I) or its salt for use in therapy wherein Ar represents a 2-hydroxyphenyl group or a pyridyl group (one or more of hydrogen atoms bound to the aromatic rings of these groups may be substituted independently with a halogen atom, an alkyl group having from 1 to 6 carbon atoms, a hydroxyl group, a hydroxyalkyl group having from 1 to 6 carbon atoms, a nitro group, an alkoxyl group having from 1 to 6 carbon atoms or a carboxyl group), R 1 represents a side chain of an amino acid selected from glutamic acid, aspartic acid, cysteic acid, homocysteic acid, glycine, alanine, β-alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, threonine, serine, homoserine, tyrosine, cysteine, methionine, glutamine, asparagine, lysine, ornithine, arginine and histidine, X represents -O- or -NH-, R 2 represents an alkyl group having from 8 to 22 carbon atoms, and n represents 0 or 1. In embodiments of the amino acid derivative represented by the above-mentioned formula (I), R 1 includes side chains of acidic amino acids such as glutamic acid, aspartic acid, cysteic acid and homocysteic acid, neutral amino acids such as glycine, alanine, β-alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, threonine, serine, homoserine, tyrosine, cysteine, methionine, glutamine and asparagine, and basic amino acids such as lysine, ornithine, arginine and histidine. The neutral amino acids are preferable, and glycine, alanine and phenylalanine are especially preferable. When an asymmetric carbon atom is present in the amino acid residue, an optically active substance or a racemic substance is available. Examples of the salt include inorganic acid salts such as a hydrochloride and a sulfate; and organic acid salts such as an acetate, a tartrate, a citrate, a p-toluenesulfonate, a fatty acid salt, an acidic amino acid salt and a pyroglutamate. These may be incorporated as amino acid derivative salts,. or the amino acid derivatives and the bases may be incorporated separately to form amino acid derivative salts in the composition. Embodiments of the amino acid derivative represented by the above-mentioned formulae (I) and (I') may easily be produced, for example, by reacting 2-hydroxy aromatic aldehyde such as salicylaldehyde with an amino acid long-chain alkyl ester or an amino acid long-chain alkylamide in the presence or absence of a solvent, and adding thereto a hydrogenation agent such as sodium boronhydride. Or, they may also be produced by reacting a 2-hydroxy aromatic aldehyde with an amino acid to form a Schiff base, adding thereto a hydrogenation agent such as sodium boronhydride to obtain N-(2-hydroxy aromatic-1-methylene)amino acid, and then subjecting the same to esterification or amidation. The 2-hydroxy aromatic aldehyde used herein includes salicylaldehyde as well as 2-hydroxy-1-naphthoaldehyde, pyridoxal, 2-hydroxy-4-methoxybenzaldehyde, o-vanillin, 5-bromosalicylaldehyde, 5-chlorosalicylaldehyde, 5-nitrosalicylaldehyde, 3,5-dibromosalicylaldehyde, 3,5-dichlorosalisylaldehyde, 2,3-dihydroxybenzaldehyde, 2,4-dihydroxybenzaldehyde and 2,5-dihydroxybenzaldehyde. The active oxygen-resisting agent of the present invention may be used by being administered directly to a system capable of generating active oxygen species. For instance, it may ordinarily be used by being incorporated in toiletries or in other compositions for external, or topical, application to the skin, such as skin treatment agents. For example, when this agent is incorporated into such products, it may be added in an amount of from 0.01 to 50% by weight, preferably 0.01 to 10% by weight. The latter range is particularly applicable should the active oxygen-resisting agent be included as an antioxidant in a toiletry product. When the active oxygen-resisting agent of the present invention is incorporated in toiletries or other compositions for topical application to the skin ingredients which are generally used in such products can be added, besides the active oxygen-resisting agent of the present invention, unless the effects of the present invention are inhibited. Examples of the ingredients which are generally used in toiletries and compositions for application to the skin include urea, ethanol, isopropanol, polyhydric alcohols, anionic surfactants, ampholytic agents, nonionic surfactants, cationic surfactants, oils, high-molecular compounds such as polymers, emulsifying agents, powders and antibiotics. The form of the compositions containing the active oxygen-resisting agent of the present invention is not particularly limited, and they may take any form such as a solution, a paste, a gel, a solid, a powder and the like. Further, the toiletries and other compositions for application to the skin containing the active oxygen-resisting agent of the present invention can take the form of washing or cleansing agents such as oils, lotions, creams, emulsions, gels, shampoos, hair rinses, hair conditioners, enamels, foundations, lipsticks, face powders, packs (eg face packs),ointments, perfumes, powders, eau de Colognes, soaps, aerosols and cleansing foams, as well as agents for preventing or improving skin ageing, agents for preventing or improving skin inflammation, bath agents or additives hair growth agents, skin care solutions, anti-sunburn agents and agents for preventing or improving skin roughness due to trauma, chaps and cracks. The other ordinary ingredients in toiletries or topically applied compositions can be added to products containing the active oxygen-resisting agent of the present invention. Examples of such ordinary ingredients include wetting agents such as sodium DL-pyrolidonecarboxylate or sodium lactate; chelating agents such as ethylenediaminetetraacetic acid, citric acid, ascorbic acid, maleic acid, succinic acid, cephalin, saccharinic acid, hexametaphosphoric acid, 1-hydroxyethane-1,1-diphosphonic acid, dihydroxyethylglycine or salts thereof; crude drugs; vitamins; hormones; agents such as antihistamic agents or skin astringents; hair growth accelerators such as cantharis tincture, capsicum tincture, ginger tincture, swertiae extract, garlic extract, hinokithiol, carpronium chloride, pentadecanoic acid glyceride, estrogen or various light-sensitive elements; beautifiers such as arbutin or other hydroquinone glycosides; antioxidants such as dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate or tocopherol; animal or vegetable extracts such as placenta extract, elastin, collagen, aloe extract, hamamelis extract, sponge cucumber extract, camomile extract or licorice extract; antiseptics such as cresol derivatives or paraben derivatives; hormones such as corticosteroid; amino acids; softeners; demulcents; strength improvers; superfatting agents; viscosity modifiers; pearling agents; antiinflammatory agents; ultraviolet absorbers; pH adjustors; flavors; fragrances; and colouring materials. [Examples] Embodiments of the present invention are illustrated more specifically by referring to the following Examples. In the Examples, the amounts are expressed as % by weight. Synthesis Example 1 Synthesis of N-(2-hydroxybenzyl)-L-alanine lauryl ester L-alanine (2.9 g) was dissolved in 20 ml of a 2-N sodium hydroxide aqueous solution, and 3.5 ml of salicylaldehyde and 0.4 g of sodium boronhydride were then added thereto in this order. After the mixture was stirred for 1 hour, 3.5 ml of salicylaldehyde and 0.4 g of sodium boronhydride were added thereto again. The mixture was stirred at room temperature for 1 hour, and the insoluble matter was then separated through filtration. The filtrate was extracted with diethyl ether. The pH was adjusted to 6 with hydrochloric acid to obtain 5.8 g of N-(2-hydroxybenzyl)-L-alanine. The resulting N-(2-hydroxybenzyl)-L-alanine (4.6 g) and 8.8 g of 1-dodecanol were added to 150 ml of toluene, and a hydrogen chloride gas was blown thereto up to the saturation. Ten grams of a molecular sieve were added thereto, and the mixture was stirred overnight. After the insoluble matter was separated through filtration, the filtrate was concentrated, and the resulting oil was dissolved in methylene chloride. The mixture was washed with a saturated aqueous solution of sodium chloride. The resulting mixture was dried over magnesium sulfate, and concentrated under reduced pressure to give 8 g of N-(2-hydroxybenzyl)-L-alanine lauryl ester. High resolution mass spectrum (M + H + ): calculated: 364.2852, found: 364.2849 1 H-NMR [CDCl 3 ] δ : 0.86 (t, 3H), 1.20 - 1.38 (m; 18H), 1.37 (d, 3H), 1.55 (m, 2H), 3.42 (q, 1H), 3.63 (6, 2H) , 3.93 (dd, 2H), 6,77 (t, 1H), 6.86 (d, 1H), 6.96 (d, 1H), 7.17 (t, 1H) Synthesis Example 2 Synthesis of N-(2-hydroxybenzyl)-L-alanine stearyl ester The above-mentioned compound was prepared in the same manner as in Synthesis Example 1. High resolution mass spectrum (M + H + ) : calculated: 448.3791, found: 448.3802 1 H-NMR [CDCl 3 ] δ : 0.86 (t, 3H), 1.19 - 1.38 (m, 30H), 1.37 (d, 3H), 1.56 (m, 2H), 3.51 (q, 1H), 3.63 (t, 2H), 4.01 (dd, 2H), 6.79 (t, 1H), 6.91 (d, 1H), 7.03 (d, 1H), 7.18 (t, 1H) Synthesis Example 3 Synthesis of N-(2-hydroxybenzyl)glycine lauryl ester The above-mentioned compound was prepared in the same manner as in Synthesis Example 1. High resolution mass spectrum (M + H + ): calculated: 350.2695, found: 350.2685 Synthesis Example 4 Synthesis of N-(2-hydroxybenzyl)-L-phenylalanine lauryl ester The above-mentioned compound was prepared in the same manner as in Synthesis Example 1. High resolution mass spectrum (M + H - ): calculated: 440.3165, found: 440.3165 Synthesis Example 5 Synthesis of N-(2-hydroxybenzyl)-L-alanine laurylamide L-alanine laurylamide (2.5 g) and 1 g of sodium hydroxide were dissolved in 20 ml of methanol, and 1.0 ml of salicylaldehyde and 0.1 g of sodium boronhydride were added thereto in this order. After the mixture was stirred for 1 hour, 1.0 ml of salicylaldehyde and 0.1 g of sodium boronhydride were added thereto again in this order. The mixture was stirred overnight at room temperature, and the insoluble matter was then separated through filtration. The filtrate was adjusted to a pH of 7 with hydrochloric acid, and concentrated under reduced pressure. The resulting oil was dissolved in diethyl ether, washed with water, and then dried over with magnesium sulfate. After the drying agent was separated through filtration, the filtrate was concentrated under reduced pressure to give 3 g of N-(2-hydroxybenzyl)-L-alanine laurylamide. High resolution mass spectrum (M + H + ): calculated: 363.3012, found: 363.2972 1 H-NMR [CDCl 3 ] δ : 0.88 (t, 3H), 1.23 - 1.35 (m, 18H), 1.50 (m, 2H), 3.13 (q, 1H), 3.27(t, 2H), 3.91 (dd, 2H), 6.78 (t, 1H), 6.83 (d, 1H), 6.96 (d, 1H), 7.19 (t, 1H) Synthesis Example 6 Synthesis of N-(2-hydroxybenzyl)-L-alanine stearylamide The above-mentioned compound was prepared in the same manner as in Synthesis Example 5. High resolution mass spectrum (M + H + ): calculated: 447.3951, found: 447.3946 1 H-NMR [CDCl 3 ] δ : 0.87 (t, 3H) , 1 .23 - 1 .36 (m, 30H), 1.38 (d, 3H), 1.51 (m, 2H), 3.23 (q, 1H), 3.33 (t, 2H), 3.97 (dd, 2H), 6.78 (t, 1H), 6.88 (d, 1H), 7.00 (d, 1H), 7.18 (t, 1H) Synthesis Example 7 Synthesis of N-(2-hydroxybenzyl)glycine laurylamide The above-mentioned compound was prepared in the same manner as in Synthesis Example 5. High resolution mass spectrum (M + H + ): calculated: 349.2855, found: 349.2865 Synthesis Example 8 Synthesis of N-(2-hydroxybenzyl)phenylalanine laurylamide The above-mentioned compound was prepared in the same manner as in Synthesis Example 5. High resolution mass spectrum (M + H + ): calculated: 439.3325, found: 439.3316 Synthesis Example 9 Synthesis of N-(2-hydroxybenzyl)-L-alanine octylamide The above-mentioned compound was prepared in the same manner as in Synthesis Example 5. High resolution mass spectrum (M + H + ): calculated: 307.2386, found: 307.2377 Test Example 1: Test for an active oxygen resistance The test was conducted according to the method described in Method in Enzymol., vol. 52, p. 302, 1978 (described also in Test Example 3 of Japanese Patent Laid-Open No. 814,755/1994 and Test Example 3 of WO 94/14755). The outline of the test method was that a homogenized 20-mM phosphate buffer of the whole brain of a C57 black mouse was prepared, a test substance was added thereto, and an absorbance of the mixture was measured. A percent inhibition by the test compound, of lipid peroxidation was calculated according to the following formula (II). The results are shown in Table 1. [Expression 1] Percent peroxidation inhibition (%) - {1 - (A 1 - A 3 ) / (A 2 - A 3 )} x 100 A 1 : Absorbance in the (presence) of the test compound A 2 : Absorbance (in the absence) of the test compound A 3 : Absorbance when the test compound is not added, nor is the heating at 37°C for 30 minutes conducted. Test compound Percent inhibition (%) Compound in Synthesis Example 1 39 Compound in Synthesis Example 2 16 Compound in Synthesis Example 5 80 Compound in Synthesis Example 6 17 N-(2-hydroxybenzyl)-L-alanine 6 Vitamin C -23 Citric acid -15 As shown in Table 1, the compounds of the present invention exhibit a higher percent inhibition of lipid peroxidation than vitamin C and citric acid as a multi-purpose antioxidant, providing a high active oxygen resistance. Test Example 2 Test for a solubility The solubility in the solvent shown in Table 2 when the concentration was 1% by weight was evaluated. Test compound Olive oil Liquid paraffin Compound in Synthesis Example 1 soluble soluble Compound in Synthesis Example 2 soluble soluble Compound in Synthesis Example 3 soluble soluble Compound in Synthesis Example 4 soluble soluble Compound in Synthesis Example 5 soluble gelling Compound in Synthesis Example 6 soluble gelling Compound in Synthesis Example 7 soluble gelling Compound in Synthesis Example 8 soluble gelling N-(2-hydroxybenzyl)-L-alanine insoluble insoluble N- (2-hydroxybenzyl) glycine insoluble insoluble N-(2-hydroxybenzyl)-L-phenylalanine insoluble insoluble As shown in Table 2, the compounds of the present invention are easily soluble in a multi-purpose olive oil of toiletries or other compositions for application to the skin by introducing a long-chain alkyl group, and are also dissolved or uniformly dispersed in liquid paraffin. Test Example 3 Organoleptic test The compounds when included in formulations for application to the skin were subjected to organoleptic evaluation by panellists, 10 men and 10 women to estimate the feeling upon use. Table 3 shows the evaluation standard of each evaluation item. Compounds shown in Table 4 were prepared in the organoleptic evaluation. Evaluation item Explanation Sticky feeling (face) o ○ : very clean Δ : sticky ○ : clean x : very sticky Sticky feeling (hands) o ○ : very clean Δ : sticky ○ : clean x : very sticky Dry and hard feeling (face) o ○ : very smooth Δ : dry and hard ○ : smooth x: very dry and hard Dry and hard feeling (hands) o ○ : very smooth Δ : dry and hard ○ : smooth x: very dry and hard Test Compound Examples Comparative Examples 1 2 3 1 2 3 Compound in Synthesis Example 1 0.1 1.0 5.0 N- (2-hydroxybenzyl)-L-alanine 1.0 5.0 Liquid paraffin 30.0 30.0 30.0 30.0 30.0 30.0 Diglycerol dioleate 5.0 5.0 5.0 5.0 5.0 5.0 Propylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 Water Evaluation Sticky feeling (face) ○ o ○ o ○ Δ ○ x Sticky feeling (hands) ○ o ○ o ○ ○ o ○ x Dry and hard feeling (face) o ○ o ○ o ○ x x ○ Dry and hard feeling (hands) o ○ o ○ o ○ ○ Δ ○ As shown in Table 4, the compounds of the present invention were all free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Example 4 A hair growth accelerator having a composition shown in Table 5 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 3 0.1 Carpronium chloride 1 Pantotenyl ethyl ether 0.5 Diphenhydramine chloride 0.1 DL-α-tocopherol 0.1 Hinokithiol 0.1 Salicylic acid 0.2 L-menthol 0.2 Glycyrrhetinic acid 0.2 Sodium DL-pyrolidonecarboxylate 1 Ethanol 50 Water balance Total 100 Example 5 Dentifrice A dentifrice having a composition shown in Table 6 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 1 0.2 Calcium hydrogenphosphate 45 Silicic anhydride 5 Glycerin 8 Sorbitol 10 Carboxymethyl cellulose 1 Sodium lauryl sulfate 1.2 Saccharin 0.1 Pigment suitable amount Antiseptic and pharmaceutical ingredient suitable amount Flavour suitable amount Water balance Total 100 Example 6 Mouth wash A mouth wash having a composition shown in Table 7 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 3 0.2 Ethanol 40 Polyoxyethylene hardened castor oil 1 Glycerin 10 1-Menthol 0.5 Sodium saccharate 0.1 Chlorhexidine gluconate suitable amount Flavour suitable amount Water balance Total 100 Example 7 Anti-sunburn cream An anti-sunburn cream having a composition shown in Table 8 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 1 1.2 Glycerin 5 Stearyl alcohol 1 Sesame oil 2 Ultraviolet absorber 5 Stearic acid 5.5 Monostearic acid glycerin 10 Antiseptic and antioxidant suitable amount Flavour suitable amount Water balance Total 100 Example 8 Acne lotion An acne lotion having a composition shown in Table 9 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 2 0.01 Compound in Synthesis Example 4 0.01 Thioxolone 0.01 Homosulfamine 0.5 Hexachlorophene 0.01 d-Camphor 0.02 1-Menthol 0.05 1,3-Butylene glycol 5 Ethanol 15 Flavor suitable amount Water balance Total 100 Example 9 Beautifier A beautifier having a composition shown in Example 10 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 2 0.5 Arbutin 0.5 Vaseline 2.5 Liquid paraffin 10 Cetostearyl alcohol 12 Polyoxyethylene (20 E.O.) sorbitan monostearate 7 Sorbitan monostearate 1 Propylene glycol 5 Antiseptic and flavor suitable amount Water balance Total 100 Examples 10 to 13 Ointment Ointments having compositions shown in Tables 11 to 14 were prepared in a usual manner. The products were free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 3 0.5 Bacitracin suitable amount Polymyxin sulfate suitable amount Polyethylene glycol distearate 15 Methyl p-oxybenzoate 0.1 Vaseline balance Total 100 Ingredients Amount Compound in Synthesis Example 2 0.5 Ethyl aminobenzoate 10 Boric acid 4 Zinc oxide 9 glycerin 4 Beeswax 20 vegetable oil balance Total 100 Ingredients Amount Compound in Synthesis Example 4 0.5 Pyridoxine hydrochloride 1 Vaseline 25 Stearyl alcohol 25 Propylene glycol 12 Sodium laurate 1 Antiseptic suitable amount Water balance Total 100 Ingredients Amount Compound in Synthesis Example 1 0.5 Hydrocortisone acetate 1 Antiseptic suitable amount Vaseline 25 Stearyl alcohol 25 Propylene glycol 12 Sodium laurate 1 Antiseptic suitable amount Water balance Total 100 Examples 14 to 16 Liquid shampoo Liquid shampoos having compositions shown in Tables 15 to 17 were prepared in a usual manner. The products were free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 1 0.1 Potassium N-lauroylglutamate 5 Potassium laurate 1 N-lauroyl-N-methyl-β-alanine sodium salt 5 Lauryl ether sodium sulfate 2 Lauroyl diethanolamide 3 Carboxyvinyl polymer 2 Collagen hydrolyzate 1 Sodium pyrolidonecarboxylate 6 Citric acid 1 Squalene 0.5 Phenoxyethanol 0.2 Methyl paraben 0.1 Disodium methylenediaminetetraacetate 0.2 Flavour suitable amount Water balance Total 100 Ingredients Amount Compound in Synthesis Example 3 1 Triethanolamine lauryl sulfate 30 Polyoxyethylene (3 E. O.) lauryl ether triethanolamine sulfate 5 Lauryldimethyl aminoacetate betaine 8 Coconut oil fatty acid amide propyl betaine 2 Diethanolamide laurate 4 Hydroxyethyl cellulose 1 Polyoxyethylene (30 E. O.) hardened castor oil 1 Glycyrrhetinic acid dipotassium salt 0.2 Dibutylhydroxytoluene 0.2 Zinc pyrithione 2 Disodium ethylenediaminetetraacetate 0.1 Flavour suitable amount Water balance Total 100 Ingredients Amount Compound in Synthesis Example 4 2 Disodium lauryl sulfosuccinate 5 Sodium dodecylbenzenesulfonate 3 Sodium lauryl phosphate 3 Triethanolamine laurate 10 Triethanolamine myristate 10 Laurylimidazolinium betaine 5 Lauroyldiethanolamide 6 Propylene glycol 7 Lauryldimethylamine oxide 2 Antiseptic suitable amount Flavour suitable amount Water balance Total 100 Example 17 Rinse A rinse having a composition shown in Table 18 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 1 0.1 Liquid paraffin 2 Lanolin alcohol 1 Vaseline 2 Cetyl alcohol 2 Glycerin monostearate 2 Polyethylene glycol (100) stearate 2 Polyoxyethylene (2 E.O.) cetyl ether 1 Polyoxyethylene (10 E. O.) octylphenyl ether 0.5 Plantalene 1200 (made by Henkel) 1 Xanthan gum 0.2 Collagen hydrolyzate 3 Lauryltrimethylammonium chloride 4 N∈-lauroyl-L-lysine ethyl ester hydrochloride 1 Citric acid suitable amount 1,3-Butylene glycol 5 Flavour suitable amount Lauryldimethylbenzylammonium chloride suitable amount Antioxidant suitable amount Water balance Total 100 Example 18 Cleansing foam A cleansing foam having a composition shown in Table 19 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 2 3 Sodium myristate 15 Potassium stearate 20 Cetanol 3 Lanolin 1 1,3-Butylene glycol 5 Glycerin 10 Potassium hydroxide 5 Antiseptic 0.1 Flavor 0.1 Water balance Total 100 Example 19 Lotion A lotion having a composition shown in Table 20 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 4 0.1 Ethanol 36 Citric acid 0.2 Zinc p-phenylsulfonate 0.2 Dipropylene glycol 3 Ethyl aminobenzoate 0.2 Menthol 0.3 Flavor 0.1 Antiseptic 0.1 Water balance Total 100 Examples 20 to 22 Beauty cream Beauty creams having compositions shown in Tables 21 to 23 were prepared in a usual manner. The products were free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 2 0.1 Stearic acid 18 Liquid paraffin 2 Jojoba oil 0.5 Sorbitan monooleate 2 Potassium hydroxide 1 Sorbitol 6 Flavor 0.1 Antiseptic 0.1 Water balance Total 100 Ingredients Amount Compound in Synthesis Example 3 0.2 Stearic acid 8 Cetyl palmitate 2 Cetanol 3 Beeswax 1 Vaseline 1 Liquid paraffin 14 Silicone oil 1 Polyoxyethylene (20 E. O.) sorbitan stearate 1 Triethanolamine 1 Propylene glycol 5 "Prodew 100" (wetting agent of Ajinomoto) 2 Flavor 0.1 Antiseptic 0.1 Water balance Total 100 Ingredients Amount Compound in Synthesis Example 4 0.1 Stearic acid 14 Beeswax 2 Liquid paraffin 1 Polyoxyethylene (20 E. O.) cetyl ether 5 Polyethylene glycol (25) monostearate 6 Allantoin chlorohydroxyammonium 0.1 Propylene glycol 5 Flavor 0.1 Antiseptic 0.1 Water balance Total 100 Example 23 Face wash A face wash having a composition shown in Table 24 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 1 2 Triethanolamine N-lauroylglutamate 25 Triethanolamine laurate 5 Polyoxypropylene (11) polyoxyethylene (4) butyl ether (HLB 7.2) 6 Dibutylhydroxytoluene 0.2 Ethanol 3 Flavour 0.3 Water balance Total 100 Example 24 Cleansing foam A cleansing foam having a composition shown in Table 25 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 2 2 Liquid paraffin 35 Ceresin 8 Beeswax 8 Cetyl i-octanoate 4 Di(cholesterol, behenyl, octyldodecylalcohol)-N-lauroyl-L-glutamate 2 Sorbitan sesquioleate 2 Polyoxyethylene (20 E. O.) sorbit beeswax 5 Sorbitol 5 Antioxidant 0.1 Antiseptic 0.1 Water balance Total 100 Example 25 Emulsion An emulsion having a composition shown in Table 26 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 4 0.2 Lauric acid 0.1 Stearic acid 0.5 Cetostearyl alcohol 0.5 Glycerin triisooctanoate 4 Avocado oil 4 Polyoxyethylene (60 E. O.) sorbitan monooleate 1.5 Glycerin monostearate 0.5 Xanthan gum (2-% aqueous solution) 7 1,3-Butylene glycol 5 Ascorbic acid suitable amount Flavour suitable amount Antiseptic 0.1 Water balance Total 100 Example 26 Lipstick A lipstick having a composition shown in Table 27 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 2 0.5 Beeswax 5 Carnauba wax 2 Candelilla wax 6 Ceresin 7 Microcrystalline wax 3 Lanolin 8 Octyldodecyl oleate 12 Isopropyl myristate 8 Titanium oxide 2 Organic pigment (tar pigment) 5 Polyglycerin 1 Antioxidant suitable amount Antiseptic and flavour suitable amount Castor oil balance Total 100 [Example 27] Hair conditioner A hair conditioner having a composition shown in Table 28 was prepared in a usual manner. The product was free from the sticky feeling and the dry and hard feeling, and the feeling upon use thereof was quite satisfactory. Ingredients Amount Compound in Synthesis Example 1 0.1 Liquid paraffin 2 Lanolin alcohol 1 Vaseline 2 Cetyl alcohol 2 Glycerin monostearate 2 Polyethylene glycol (100) stearate 2 Polyoxyethylene (2 E.O.) cetyl ether 1 Polyoxyethylene (10 E. O.) octylphenyl ether 0.5 Plantalene 1200 (made by Henkel) 1 Xanthan gum 0.2 Collagen hydrolyzate 3 Lauryltrimethylammonium chloride 4 N∈-lauroyl-L-lysine ethyl ester hydrochloride 1 Citric acid suitable amount 1,3-Butylene glycol 5 Flavour suitable amount Lauryldimethylbenzylammonium chloride suitable amount Antioxidant suitable amount Water balance Total 100 [Effects of the Invention] The novel active oxygen-resisting agent of the present invention has an excellent active oxygen resistance, and exhibits a good solubility in an oil solvent. Further, toiletries or an external agent containing the active oxygen resisting agent remains effectively on the skin, is hardly dropped when coated on the skin or the hair, and has a smooth feeling upon use without providing a dry and hard feeling on the skin and the hair.

Claims: An amino acid derivative represented by the following formula (I') or its salt wherein Ar represents a 2-hydroxyphenyl group or a pyridyl group (one or more of hydrogen atoms bound to the aromatic rings of these groups may be substituted independently with a halogen atom, an alkyl group having from 1 to 6 carbon atoms, a hydroxyl group, a hydroxyalkyl group having from 1 to 6 carbon atoms, a nitro group, an alkoxyl group having from 1 to 6 carbon atoms or a carboxyl group), R 1 represents a side chain of an amino acid selected from glutamic acid, aspartic acid, cysteic acid, homocysteic acid, glycine, alanine, β-alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, threonine, serine, homoserine, tyrosine, cysteine, methionine, glutamine, asparagine, lysine, ornithine, arginine and histidine, X represents -O- or -NH-, R 2 represents an alkyl group having from 8 to 22 carbon atoms, and n represents 0 or 1; with the proviso that when Ar-CH 2 - is an unsubstituted picolyl group, R 1 is hydrogen, n is 0 and X is O, then R 2 is not a -2,6 dimethylheptyl, a dodecyl, or a hexadecyl group. An amino acid derivative or its salt according to claim 1 wherein Ar represents a substituted or unsubstituted 2-hydroxyphenyl group or a substituted pyridyl group. An amino acid derivative represented by the following formula (I) or its salt for use in therapy wherein Ar represents a 2-hydroxyphenyl group or a pyridyl group (one or more of hydrogen atoms bound to the aromatic rings of these groups may be substituted independently with a halogen atom, an alkyl group having from 1 to 6 carbon atoms, a hydroxyl group, a hydroxyalkyl group having from 1 to 6 carbon atoms, a nitro group, an alkoxyl group having from 1 to 6 carbon atoms or a carboxyl group), R 1 represents a side chain of an amino acid selected from glutamic acid, aspartic acid, cysteic acid, homocysteic acid, glycine, alanine, β-alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, threonine, serine, homoserine, tyrosine, cysteine, methionine, glutamine, asparagine, lysine, ornithine, arginine and histidine, X represents -O- or -NH-, R 2 represents an alkyl group having from 8 to 22 carbon atoms, and n represents 0 or 1. The use of an amino acid derivative or salt according to claim 3 in the manufacture of a medicament for topical application to the body for the prevention, treatment, or inhibition of the action of active oxygen. The use according to claim 4 wherein the prevention, treatment, or inhibition of the action of active oxygen is in the course of treatment of skin cancer, pigmentation or inflammation. A cosmetic method comprising topically applying to the body an amino acid derivative represented by formula (I) as set out in claim 3 or its salt. A composition for inhibiting, preventing or treating the action of active oxygen comprising an amino acid derivative of formula (I) as set out in claim 3 or its salt and an excipient, diluent or carrier suitable for topical application to the human body. A composition according to claim 7 which is a skin anti-aging agent. A composition according to claim 7 which is a toiletry. A composition according to claim 7 which is a skin treatment agent. Use of an amino acid derivative or salt of formula (I) as set out in claim 3 for inhibiting or preventing the action of active oxygen. A method for the production of an amino acid derivative having formula (I) as set out in claim 3 comprising: (a) reacting a 2-hydroxy aromatic aldehyde with an amino acid long-chain alkyl ester or an amino acid long-chain alkylamide, in the presence or absence of a solvent and hydrogenating; or (b) reacting a 2-hydroxy aromatic aldehyde with an amino acid to form a schiff base, hydrogenating to obtain N-(2-hydroxy aromatic-1-methylene) amino acid and then subjecting the same to esterification or amidation.

Inventors: IWASAKI KEIJI, KITAZAWA MANABU, SAKAMOTO KAZUTAMI, IWASAKI, KEIJI, KITAZAWA, MANABU, SAKAMOTO, KAZUTAMI, Iwasaki, Keiji, c/o Ajinomoto Co., Inc., Kitazawa, Manabu, c/o Ajinomoto Co., Inc., Sakamoto, Kazutami, c/o Ajinomoto Co., Inc.
Applicants: AJINOMOTO KK, AJINOMOTO CO., INC.
Application year: 1998
Main classification: C07C229
IPC codes: C07C237, A61Q1, A61P17, A61K8, C07C229, A61Q5, A61K31, C09K15, A61Q11, A61Q19