alpha -O-LINKED GLYCOCONJUGATES WITH CLUSTERED (2,6)-ST EPITOPES, METHODS OF PREPARATION AND USES THEREOF

Abstract: The present invention provides novel alpha -O-linked glycoconjugates such as alpha -O-linked glycopeptides, as well as convergent methods for synthesis thereof. The general preparative approach is exemplified by the synthesis of the mucin motif commonly found on epithelial tumor cell surfaces. The present invention further provides compositions and methods of treating cancer using the alpha -O-linked glycoconjugates.

Description: α-O-LINKEP GLYCOCONIUGATES WITH CLUSTERED (2.6VST EPITOPES. METHODS OF PREPARATION AND USES THEREOF This application is based on U.S. Provisional Application Serial No. 60/043,713, filed April 16, 1997, the contents of which are hereby incorporated by reference into this application. This invention was made with government support under grants CA-28824, HL-25848 and Al- 16943 from the National Institutes of Health. Accordingly, the U.S. Government has certain rights in the invention. Field of the Invention The present invention is in the field of α-O-linked glycopeptides. In particular, the present invention relates to methods for the preparation of α-O-linked glycoconjugates with clustered glycodomains which are useful as anticancer therapeutics. The present invention also provides novel compositions comprising such α-O-linked glycoconjugates and methods for the treatment of cancer using these glycoconjugtes. Throughout this application, various publications are referred to, each of which is hereby incorporated by reference in its entirety into this application to more fully describe the state of the art to which the invention pertains. Background of the Invention The role of carbohydrates as signaling molecules in the context of biological processes has recently gained prominence. M.L. Phillips, et al., Science, 1990, 250, 1130; M.J. Polley, et al., Proc. Natl. Acad. Sci. USA, 1991 88, 6224: T. Taki, et al., ). Biol. Chem., 1996, 267, 3075; Y. Hirabayashi, A. Hyogo, T. Nakao, K. Tsuchiya, Y. Suzuki, M. Matsumoto, K. Kon, S. Ando, ibid., 1990, 265, 8144; O. Hindsgaul, T. Norberg, J. Le Pendu, R.U. Lemieux, Carbohydr. Res. 1982, 109, 109; U. Spohr, R.U. Lemieux, ibid., 1988, 174, 211). The elucidation of the scope, of carbohydrate involvement in mediating cellular interaction is an important area of inquiry in contemporary biomedical research. The carbohydrate molecules, carrying detailed structural information, tend to exist as glycoconjugates (cf. glycoproteins and glycolipids) rather than as free entities. Given the complexities often associated with isolating the conjugates in homogeneous form and the difficulties in retrieving intact carbohydrates from these naturally occurring conjugates, the applicability of synthetic approaches is apparent. (For recent reviews of glycosylation see: Paulsen, H.; Angew. Chemie Int. Ed. Engl. 1982, 21, 155; Schmidt, R.R., Angew. Chemie Int. Ed. Engl. 1986, 25, 212; Schmidt, R.R., Comprehensive Organic Synthesis, Vol. 6, Chapter 1 (2), Pergamon Press, Oxford, 1991; Schmidt, R.R., Carbohydrates, Synthetic Methods and Applications in Medicinal Chemistry, Part I, Chapter 4, VCH Publishers, Weinheim, New York, 1992. For the use of glycals as glycosyl donors in glycoside synthesis, see Lemieux, R.U., Can. J. Chem., 1964, 42, 141 7; Lemieux, R.U., Fraiser- Reid, B., Can. J. Chem. 1965, 43, 1460; Lemieux, R.U.; Morgan, A.R., Can. /. Chem. 1965, 43, 2190; Thiem, J., et al., Synthesis 1978, 696; Thiem, J. Ossowski, P., Carbohydr. Chem., 1984, 3, 287; Thiem, J., et al., Liebigs Ann. Chem., 1986, 1044; Thiem, J. in Trends in Synthetic Carbohydrate Chemistry, Horton, D., et al., eds., ACS Symposium Series No. 386, American Chemical Society, Washington, D.C., 1989, Chapter 8.) The carbohydrate domains of the blood group substances contained in both glycoproteins and glyco pids are distributed in erythrocytes, epithelial cells and various secretions. The early focus on these systems centered on their central role in determining blood group specificities. R.R. Race; R. Sanger, Blood Groups in Man, 6th ed., Blackwell, Oxford, 1975. However, it is recognized that such determinants are broadly implicated in cell adhesion and binding phenomena. (For example, see M.L. Phillips, et al., Science 1990, 250, 1 130.) Moreover, ensembles related to the blood group substances in conjugated form are encountered as markers for the onset of various tumors. K.O. Lloyd, Am. ]. Clinical Path., 1987, 87, 129; K.O. Lloyd, Cancer Biol., 1991, 2, 421. Carbohydrate-based tumor antigenic factors have applications at the diagnostic level, as resources in drug delivery or ideally in immunotherapy. Toyokuni, T., et al., j. Am. Chem Soc. 1994, 1 16, 395; Dranoff, G., et al., Proc. Natl. Acad. Sci. USA 1993, 90, 3539; Tao, M-H.; Levy, R., Nature 1993, 362, 755; Boon, T., Int. j. Cancer 1993, 54, 1 77; Livingston, P.O., Cur r. Opin. Immunol. 1992, 4, 624; Hakomori, S., Annu. Rev. Immunol. 1984, 2, 103; K. Shigeta, et al., ). Biol. Chem. 1987, 262, 1358. The present invention provides new strategies and protocols for glycopeptide synthesis. The object is to simplify such preparations so that relatively complex domains can be assembled with high stereospecifity. Major advances in glycoconjugate synthesis require the attainment of a high degree of convergence and relief from the burdens associated with the manipulation of blocking groups. Another requirement is that of delivering the carbohydrate determinant with appropriate provision for conjugation to carrier proteins or lipids. Bernstein, M.A.; Hall, L.D., Carbohydr. Res. 1980, 78, Cl; Lemieux, R.U., Chem. Soc. Rev. 1978, 7, 423; R.U. Lemieux, et al., \. Am. Chem. Soc. 1975, 97, 4076. This is a critical condition if the synthetically derived carbohydrates are to be incorporated into carriers suitable for clinical application. Antigens which are selective (or ideally specific) for cancer cells could prove useful in fostering active immunity. Hakomori, S., Cancer Res., 1985, 45, 2405-2414; Feizi, T., Cancer Surveys 1985, 4, 245-269. Novel carbohydrate patterns are often presented by transformed cells as either cell surface glycoproteins or as membrane-anchored glycolipids. In principle,, well chosen synthetic glycoconjugates which stimulate antibody production could confer active immunity against cancers which present equivalent structure types on their cell surfaces. Dennis, J., Oxford Clycostems Clyconews, Second Ed., 1992; Lloyd, K.O., in Specific Immunotherapy of Cancer with Vaccines, 1993, New York Academy of Sciences, pp.50-58. Chances for successful therapy improve with increasing restriction of the antigen to the target cell. For example, one such specific antigen is the glycosphingolipid isolated by Hakomori and collaborators from the breast cancer cell line MCF-7 and immunocharacterized by monoclonal antibody MBrl. Bremer, E.G., et al., J. Biol. Chem. 1984, 259, 14773-14777; Menard, S., et al., Cancer Res. 1983, 43, 1295-1300. The surge of interest in glycoproteins (M.J. McPherson, et al., eds., PCR A Practical Approach, 1994, Oxford University Press, Oxford, G.M. Blackburn; M.J. Gait, Eds., Nucleic Acids in Chemistry and Biology, 1990, Oxford University Press, Oxford; A.M. Bray; A.G. Jhingran; R.M. Valero; N.J. Maeji, /. Org. Chem. 1944, 59, 2197; G. Jung; A.G. Beck- Sickinger, Angew Chem. Int. Ed. Engl. 1992, 37, 367; M.A. Gallop; R.W. Barrett; W.J. Dower; S.P.A. Fodor; E.M. Gordon, /. Med. Chem. 1994, 37, 1233; H.P. Nestler; P.A. Bartlett; W.C. Still, ]. Org. Chem. 1994, 59, 4723; M. Meldal, Curr. Opin. Struct. Biol. 1994, 4, 673) arises from heightened awareness of their importance in diverse biochemical processes including cell growth regulation, binding of pathogens to cells (O.P. Bahl, in Glycoconjugates: Composition, structure, and function, H. J. Allen, E.C. Kisailus, Eds., 1992, Marcel Dekker, Inc., New York, p. 1), intercellular communication and metastasis (A. Kobata, Ace. Chem. Res. 1993, 26, 31 ). Glycoproteins serve as cell differentiation markers and assist in protein folding and transport, possibly by providing protection against proteolysis. G. Opdenakker, et al., FASEB j. 1993, 7, 1330. Improved isolation techniques and structural elucidation methods (A. De; K.-H. Khoo, Curr. Opin. Struct. Biol. 1993, 3, 687) have revealed high levels of microheterogeneity in naturally-produced glycoproteins. R.A. Dwek, et al., Annu. Rev. Biochem. 1993, 62, 65. Single eukaryotic cell lines often produce many glycoforms of any given protein sequence. For instance, erythropoietin (EPO), a clinically useful red blood cell stimulant against anemia, is glycosylated by more than 13 known types of oligosaccharide chains when expressed in Chinese hamster ovary cells (CHO) (Y.C. Lee; R.T. Lee, Eds., Neoglycoconjugates: Preparation and Applications, 1994, Academic Press, London). The efficacy of erythropoietin is heavily dependent on the type and extent of glycosylation (E. Watson, et al., Glycobiology, 1994, 4, 227). Elucidation of the biological relevance of particular glycoprotein oligosaccharide chains requires access to pure entities, heretofore obtained only by isolation. Glycoprotein heterogeneity renders this process particularly labor-intensive. However, particular cell lines can be selected to produce more homogeneous glycoproteins for structure-activity studies. U.S. Patent No. 5,272,070. However, the problem of isolation from natural sources remains difficult. Receptors normally recognize only a small fraction of a given macromolecular glycoconjugate. Consequently, synthesis of smaller but well-defined putative glycopeptide ligands could emerge as competitive with isolation as a source of critical structural information (Y.C. Lee; R.T. Lee, Eds., supra). Glycoconjugates prepared by total synthesis are known to induce mobilization of humoral responses in the murine immune system. Ragupathi, G., et al., Angew. Chem. Int. Ed. Engl. 1997, 36, 125; Toyokuni, T.; Singhal, A.K., Chem. Soc. Rev. 1995, 24, 231 ; Angew. Chem. Int. Ed. Engl. 1996, 35, 1381. Glycopeptides, in contrast to most glycolipids and carbohydrates themselves, are known to bind to major histocompatability complex (MHC) molecules and stimulate T cells in favorable cases. Deck, B., et al., I. Immunology 1995, 1074; Haurum, J.S., et al., ). Exp. Med. 1994, 780, 739; Sieling, P.A., et al., Science 1995, 269, 227 (showing T cell recogniztion of CD 1 -restricted microbial glycolipid). Properly stimulated T cells express receptors that specifically recognize the carbohydrate portion of a glycopeptide. The present invention demonstrates a means of augmenting the immunogenicity of carbohydrates by use of a peptide attachment. Preparation of chemically homogeneous glycoconjugates, including glycopeptides and glycoproteins, constitutes a challenge of high importance. Bill, R.M.; Flitsch, S.L.; Chem. & Biol. 1996, 3, 145. Extension of established cloning approaches to attain these goals are being actively pursued. Various expression systems (including bacteria, yeast and cell lines) provide approaches toward this end, but, as noted above, produce heterogeneous glycoproteins. Jenkins, N., et al., Nature Biotech. 1996, 74, 975. Chemical synthesis thus represents a preferred avenue to such bi-domainal constructs in homogeneous form. Moreover, synthesis allows for the assembly of constructs in which selected glycoforms are incorporated at any desired position of the peptide chain. Prior to the subject invention, methods of glycopeptide synthesis pioneered by Kunz and others allowed synthetic access to homogenous target systems both in solution and solid phase (M. Meldal, Curr. Opin. Struct. Biol, 1994, 4, 710; M. Meldal, in Neoglycoconjugates: Preparation and Applications, supra; S.J. Danishefsky; j.Y. Roberge, in Clycopeptides and Related Compounds: Chemical Synthesis, Analysis and Applications, 1995, D.G. Large, CD. Warren, Eds., Marcel Dekker, New York; S.T. Cohen-Anisfeld and P.T. Lansbury, Jr., /. Am. Chem. Soc, 1993, 7 75, 10531 ; S.T. Anisfeld; P.T. Lansbury Jr., ). Org. Chem, 1990, 55, 5560; D. Vetter, et al., Angew. Chem. Int. Ed. Engl, 1995, 34, 60-63). Cohen-Anisfeld and Lansbury disclosed a convergent solution-based coupling of selected already available saccharides with peptides. S.T. Cohen-Anisfeld; P.T. Lansbury, Jr., }. Am. Chem. Soc, supra. Thus, few effective methods for the preparation of α-O-linked glycoconjugates were known prior to the present invention. Nakahara, Y., et al., In Synthetic Oligosaccharides, ACS Symp. Ser. 560, 1994, pp. 249-266; Garg, H.G., et al., Adv. Carb. Chem. Biochem. 1994, 50, 277. Nearly all approaches incorporated the amino acid (serine or threonine) at the monosaccharide stage. This construction would be followed by elaboration of the peptidyl and carbohydrate domains in a piecemeal fashion. Qui, D.; Koganty, R.R.; Tetrahedron Lett. 1997, 38, 45. Eloffson, M., et al., Tetrahedron 1997, 53, 369. Meinjohanns, E., et al., ]. Chem. Soc, Perkin Trans. 1, 1996, 985. Wang, Z-G., et al., Carbohydr. Res. 1996, 295, 25. Szabo, L, et al., Carbohydr. Res. 1995, 274, 1 1. The scope of the synthetic problem is well known in the art, but little progress has been achieved. The present invention provides an alternate, simpler and more convergent approach (Figure 2). Toyokuni et al., J.Amer.Chem.Soc, 1994, 7 76, 395, have prepared synthetic vaccines comprising dimeric Tn antigen-lipopeptide conjugates having efficacy in eliciting an immune response against Tn-expressing glycoproteins. However, prior to investigations of the present inventors, it was not appreciated that the surface of prostate cancer cells presents glycoproteins comprising Tn clusters linked via threonine rather than serine residues. Accordingly, the present invention provides a vaccine having unexpectedly enhanced anticancer efficacy. Summary of the Invention Accordingly, one object of the present invention is to provide novel α-O- linked glycoconjugates including glycopeptides and related compounds which are useful as anticancer therapeutics. Another object of the present invention is to provide synthetic methods for preparing such glycoconjugates. An additional object of the invention is to provide compositions useful in the treatment of subjects suffering from cancer comprising any of the glycoconjugates available through the preparative methods of the invention, optionally in combination with pharmaceutical carriers. The present invention is also intended to provide a fully synthetic carbohydrate vaccine capable of fostering active immunity in humans. A further object of the invention is to provide methods of treating subjects suffering from of cancer using any of the glycoconjugates available through the preparative methods of the invention, optionally in combination with pharmaceutical carriers. Brief Description of the Drawings Figure 1 shows a schematic structure for α-O-linked glycoconjugates as present in mucins. Figure 2 provides a general synthetic strategy to mucin glycoconjugates. Figure 3 provides a synthetic route to prepare key intermediate β-phenylthioglycoside 11. Reaction conditions: (a) (1) DMDO, CH 2 CI 2 ; (2) 6-O-TIPS-galactal, ZnCI 2 , -78°C to 0°C; (3) Ac 2 0, Et 3 N, DMAP, 75%; (b) TBAF/AcOH/THF; 80%; (c) 5 (1.3 eq), TMSOTf (0.1 eq), THF:Toluene 1 :1 , -60°C to -45°C, 84%, α:β 4: . ; (d) NaN 3 , CAN, CH 3 CN, -15°C, 60%; (e) LiBr, CH j CN, 75%; (f) (1 ) 1 PhSH, iPr 2 NEt, CH 3 CN, 82% (2) CCI 3 CN, K 2 C0 3 , CH 2 CI 2 , 80%; (g) (1) PhSH, iPr 2 NEt; (2) CIP(OEt) 2 , iPr 2 NEt, THF, (labile compd, -72% for two steps); (h) (1) LiBr, CH 3 CN, 75%; (2) LiSPh, THF, 0°C, 70%). Figure 4 presents a synthetic route to glycoconjugate mucin 1. Reaction conditions: (a) CH 3 COSH, 78%; (b) H 2 / 10% Pd-C, MeOH, H 2 0, quant.; (c) H 2 N- Ala-Val-OBn, liDQ, CH 2 CI 2 , 85%; (d) KF, DMF, 18-crown-6, 95%; (e) 15, IIDQ, 87%; (0 KF, DMF, 18-crown-6,93%; (g) 14, IIDQ, 90%; (h) (1) KF, DMF, 18-crown-6; (2) Ac 2 0, CH 2 CI 2 ,; (i) H 2 / 10% Pd-C, MeOH, H 2 0, 92% (three steps); (j) NaOH, H 2 0, 80%., Figure 5 shows a synthetic route to prepare glycoconjugates by a fragment coupling. Reagents: (a) IIDQ, CH 2 CI 2 , rt, 80%; (b) H j /Pd-C, MeOH, H 2 0, 95%; (c) CF 3 COOH, CH 2 CI 2 ; (d) NaOH, H 2 0, MeOH. Figure 6 shows the synthesis of α-O-linked glycopeptide conjugates of the Le y epitope via an iodosulfonamidation/4 + 2 route. Figure 7 provides the synthesis of α-O-linked glycopeptide conjugates of the Le y epitope via an azidonitration/4 + 2 route. Figures 8 and 9 present examples of glycopeptides derived by the method of the invention. Figure 10 illustrates a synthetic pathway to prepare glycopeptides ST N and T(TF). Figure 11 shows a synthetic pathway to prepare glycopeptide (2,3)ST. Figure 12 shows a synthetic pathway to prepare the glycopeptide glycophorine. Figure 13 presents a synthetic pathway to prepare glycopeptides 3-Le y and 6-Le y . Figure 14 provides a synthetic pathway to prepare T-antigen. Figure 15 shows a synthetic pathway to prepare the alpha cluster of the T-antigen. Figure 16 shows a synthetic pathway to prepare the beta cluster of the T-antigen. The sequence of reactions are as represented in Figure 15. Figures 17, 18 and 19 presents a synthesis of α-O-linked glycopeptide conjugates of the Le y epitope. R is defined in Figure 18. Figure 20 shows (A) the conjugation of Tn-trimer glycopeptide to PamCys lipopeptide; (B) a general representation of a novel vaccine construct; and (C) a PamCys Tn Trimer. Figure 21 illustrates (A) a method of synthesis of a PamCys-Tn-trimer 3; and (B) a method of preparation of KLH and BSA conjugates (12, 13) via cross-linker conjugation. Figure 22 shows (A) a mucin related F1 α antigen and a retrosynthetic approach to its preparation; and (B) a method of preparing intermediates 5' and 6'. conditions: i) NaN 3 , CAN, CH 3 , CN, -20 °C, overnight, 40%, α (4a ' ): β (4b ' ) 1 :1 ; ii) PhSH, EtN(i-Pr) 2 , CH 3 ,CN, 0 °C, 1 h, 99.8%, iii) K 2 C0 3 , CC1 3 ,CN, CH 2 CI 2 , rt, 5h, 84%, 5a ' : 5b ' 1 :5;iv) DAST, CH 2 C1 2 , 0 °C, 1h, 93%, 6a ' : 6b ' 1:1. Figure 23 shows a method of preparing intermediates 1 ' and 2'. Conditions: i) TBAF, HOAc, THF, rt, 3d, 100% yield for 9 ', 94% yield for 10 ' ; ii) 11 ', BF 3 ,Et 2 0, -30 °C, overnight; iii) AcSH, pyridine, rt, overnight, 72% yield yield based on 50% conversion of 1 1 ' , 58% yield based on 48% conversion of 12 ' (two steps); iv) 80% aq. HOAc, overnight, rt-40 °C; v) Ac 2 0, pyridine, rt., overnight; vi) 10% Pd/C, H 2 , MeOH-H 2 0, rt, 4h; vii) Morpholine, DMF, rt, overnight; viii) NaOMe, MeOH-THF, rt, overnight, 64% yield for 1 ' , 72% yield for 2 ' (five steps). Figure 24 shows a method of preparing intermediates in the synthesis of F1 α antigen. Conditions: i) (sym-collidine) 2 CI0 4 , PhS0 2 NH 2 , 0 °C; LiHMDS < EtSH, -40 °C-rt, 88% yield in two steps; ii) MeOTf, DTBP, 0 °C, 86% yield for 20 ' plus 8% yield of α isomer; 85% yield for 21 ;plus 6% yield of α isomer; iii) Na, NH 3 , 78° C; Ac 2 02, Py, rt, for 22 ', 59% yield in two steps; iv) NaN 3 , CAN, CH 3 CN, -20 ° C; v) PhSH, EtN(i-Pr) 2 ; Ccl 3 CN, K 2 C0 3 ; for 23 ', 17 % yield of 2:7, α/β in three steps; for 24 ' 30% yield of 3;1 , α/β in three steps; vi) LiBr, CH 3 CN, for 25 ' , 46% yield, α only; vii) Ac 2 0, Py; Na-Hg, Na 2 HP0 4 , 94% yield in two steps, NaN 3 , CAN, 26% yield, PhSH, EtN(i-Pr) 2 ; K 2 C0 3 , Ccl 3 CN, 53% yield in two steps (27 '); viii) LiSph, THF, 60% yield, β only (26 ' ). Detailed Description of the Invention The subject invention provides novel α-O-linked glycoconjugates, useful in the prevention and treatment of cancer. The present invention provides a glycoconjugate having the structure: A-B m -C n -D p -E q -F wherein m, n, p and q are 0, 1 , 2 or 3 such that m + n + p + q ≤ 6; wherein A, B, C, D, E and F are independently amino acyl or hydroxy acyl residues wherein A is N- or O-terminal and is either a free amine or ammonium form when A is amino acyl or a free hydroxy when A is hydroxy acyl, or A is alkylated, arylated or acylated; wherein F is either a free carboxylic acid, primary carboxamide, mono- or dialkyl carboxamide, mono- or diarylcarboxamide, linear or branched chain (carboxy)alkyl carboxamide, linear or branched chain (alkoxycarbonyl)alkyl-carboxamide, linear or branched chain (carboxy)arylalkylcarboxamide, linear or branched chain (alkoxycarbonyl)alkylcarboxamide, an oligoester fragment comprising from 2 to about 20 hydroxy acyl residues, a peptidic fragment comprising from 2 to about 20 amino acyl residues, or a linear or branched chain alkyl or aryl carboxylic ester; wherein from one to about five of said amino acyl or hydroxy acyl residues are substituted by a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1, 2 or 3; wherein the carbohydrate domain is linked to the respective amino acyl or hydroxy acyl residue by substitution of a side group substituent selected from the group consisting of OH, COOH and NH 2 ; wherein R n is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR', NH 2 , NHCOR', F, CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R,„ R, 2 , R 13 , R,« and R 1S are each independently hydrogen, OH, OR"', NH 2 , NHCOR"', F, CH 2 OH, CH 2 OR'", or a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R, 6 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R'" is hydrogen, CHO, COOR 1 ", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein R" and R 1 * are each independently H, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group. In a certain embodiment, the present invention provides the glycoconjugate as shown above wherein at least one carbohydrate domain has the oligosaccharide structure of a cell surface epitope. In a particular embodiment, the present invention provides the glycoconjugate wherein the epitope is Le a , Le b , Le", or Le y . In another particular embodiment, the present invention provides the glycoconjugate wherein the epitope is MBr1 , a truncated MBr1 pentasaccharide or a truncated MBr1 tetrasaccharide. In another embodiment, the present invention provides a glycoconjugate wherein the amino acyl residue is derived from a natural amino acid. In another embodiment, the invention provides the glycoconjugate wherein at least one amino acyl residue has the formula: -N H-Ar-CO-. In a specific embodiment, the Ar moiety is p- phenylene. In another embodiment, the present invention provides the glycoconjugate wherein at least one amino acyl or hydroxy acyl residue has the structure: wherein M, N and P are independently 0, 1 or 2; X is NH or O; Y is OH, N H or COOH; and wherein R' and R" are independently hydrogen, linear or branched chain alkyl or aryl. In a specific embodiment, the amino acyl residue attached to the carbohydrate domain is Ser or Thr. In another embodiment, the present invention provides the glycoconjugate wherein one or more of R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R, 0 , R , R 12 , R 13 , R, 4 and R 15 is 1 RS,2R5,3-trihydroxy-propyl. The present invention also provides a pharmaceutical composition for treating cancer comprising the above-shown glycoconjugate and a pharmaceutically suitable carrier. The present invention further provides a method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of the above-shown glycoconjugate and a pharmaceutically suitable carrier. The method of treatment is effective when the cancer is a solid tumor or an epithelial cancer. The present invention also provides a trisaccharide having the structure: wherein R,, R 3 , R 4 , R 5 , R 6 and R 7 are each independently hydrogen, OH, OR', NH 2 , NHCOR', F, N 3 , CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is H, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; wherein R 2 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R 8 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R" is a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein X is a halide, a trihaloacetamidate, an alkyl or aryl sulfide or a dialkylphosphite. In a preferred embodiment, the invention provides the above-shown trisaccharide wherein X is a triethylphosphite. The invention further provides the trisaccharide wherein R 7 is 1 R5,2R5,3-trihydroxypropyl or 1 R5,2/.5,3-triacetoxypropyl. In addition, the invention provides the trisaccharide wherein R 8 is COOH. The present invention also provides a trisaccharide amino acid having the structure: wherein R„ R 3 , R 4 , R 5 , R 6 and R 7 are each independently hydrogen, OH, OR', NH 2 , NHCOR 1 , F, N 3 , CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is H, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; wherein R 2 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R β is hydrogen, COOH, COOR", CON HR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R" is a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; wherein R 0 is a base-labile N-protecting group; and wherein R' is hydrogen or a lower alkyl group. A variety of N-protecting groups would be acceptable in the preparation of the above-shown trisaccharide amino acid. R 0 may preferably be one of several base-sensitive protecting groups, but more preferably fluorenylmethyloxycarbonyl (FMOC). The present invention provides a method of inducing antibodies in a human subject, wherein the antibodies are capable of specifically binding with human tumor cells, which comprises administering to the subject an amount of the glycoconjugate disclosed herein effective to induce the antibodies. In a certain embodiment, the present invention provides a method of inducing antibodies wherein the glycoconjugate is bound to a suitable carrier protein. In particular, preferred examples of the carrier protein include bovine serum albumin, polylysine or KLH. In another embodiment, the present invention contemplates a method of inducing antibodies which further comprises co-administering an immunological adjuvant. In a certain embodiment, the adjuvant is bacteria or liposomes. Specifically, favored adjuvants include Salmonella minnesota cells, baciile Calmette-Guerin or QS21. The antibodies induced are typically selected from the group consisting of (2,6)-sialyl T antigen, Le a , Le b , Le , Le y , GM1 , SSEA-3 and MBrl antibodies. The method of inducing antibodies is useful in cases wherein the subject is in clinical remission or, where the subject has been treated by surgery, has limited unresected disease. The present invention also provides a method of preventing recurrence of epithelial cancer in a subject which comprises vaccinating the subject with the glycoconjugate shown above which amount is effective to induce antibodies. In practicing this method, the glycoconjugate may be used alone or be bound to a suitable carrier protein. Specific examples of carrier protein used in the method include bovine serum albumin, polylysine or KLH. In a certain embodiment, the present method of preventing recurrence of epithelial cancer includes the additional step of co-administering an immunological adjuvant. In particular, the adjuvant is bacteria or liposomes. Favored adjuvants include Salmonella minnesota cells, baciile Calmette-Guerin or QS21. The antibodies induced by the method are selected from the group consisting of (2,6)-sialyl T antigen, Le a , Le b , Le", Le y , GM1 , SSEA-3 and MBrl antibodies. The present invention further provides a glycoconjugate having the structure: wherein X is O or NR; wherein R is H, linear or branched chain alkyl or acyl; wherein A, B and C independently linear or branched chain alkyl or acyl, -CO-(CH 2 ) P -OH or aryl, or have the structure: wherein Y is O or NR; wherein D and E have the structure: -(CH 2 ) P -OH or -CO-(CH 2 ) P -OH; wherein N and P are independently an integer between 0 and 12; wherein D and E and, when any of A, B and C are -CO-(CH 2 ) P -OH, A, B and C are independently substituted by a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein the carbohydrate domain is linked to the respective hydroxy acyl residue by substitution of a terminal OH substituent; wherein R 0 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR', NH 2 , NHCOR', F, CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R , R 12 , R 13 , R 14 and R 15 are each independently hydrogen, OH, OR"', NH 2 , HCOR'", F, CH 2 OH, CH 2 OR ni , or a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R 16 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R"' is hydrogen, CHO, COOR iv , or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein R" and R iv are each independently H, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group. In a certain embodiment, the present invention provides the above-shown glycoconjugate wherein at least one carbohydrate domain has the oligosaccharide structure of a cell surface epitope. In one embodiment, the epitope is Le a , Le b , Le x , or Le y . In another embodiment, the epitope is MBrl , a truncated MBrl pentasaccharide or a truncated MBrl tetrasaccharide. In a particular embodiment, the invention provides the glycoconjugate shown above wherein one or more of R„ R 2 , R 3 , R 4 , R s, R β / R _ R 8 R _ R ιo/ R n R i 2 R i 3 R i ar| d R 15 is 1 RS,2R5,3-trihydroxy-propyl. The invention also provides a pharmaceutical composition for treating cancer comprising the glycoconjugate shown above and a pharmaceutically suitable carrier. The invention further provides a method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of the glycoconjugate shown above and a pharmaceutically suitable carrier. The method is useful in cases where the cancer is a solid tumor or an epithelial cancer. The present invention also provides a glycoconjugate comprising a core structure and a carbohydrate domain wherein the core structure is: wherein M is an integer from about 2 to about 5,000; wherein N is 1 , 2, 3 or 4; wherein A and B are suitable polymer termination groups, including linear or branch chain alkyl or aryl groups; wherein the core structure is substituted by the carbohydrate domain having the structure: J z wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein the carbohydrate domain is linked to the core structure by substitution of the OH substituents; wherein R 0 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR', NH 2 , NHCOR 1 , F, CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or trijhydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: w wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R,„ R, 2 , R 13 , R 14 and R 15 are each independently hydrogen, OH, OR"', NH 2 , NHCOR'", F, CH 2 OH, CH 2 OR hi , or a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or trijhydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R 16 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R" 1 is hydrogen, CHO, COOR iv , or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein R" and R' v are each independently H, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group. In a specific embodiment, the present invention provides a method of preparing glycopeptides related to the mucin family of cell surface glycoproteins. Mucins are characterized by aberrant α-O-glycosidation patterns with clustered arrangements of carbohydrates α-O-linked to serine and threonine residues. Figure 1. Mucins are common markers of epithelial tumors (e.g., prostate and breast carcinomas) and certain blood cell tumors. Finn, O.J., et al., Immunol. Rev. 1995, 745, 61. The (2,6)- Sialyl T antigen (ST antigen) is an example of the "glycophorin family" of α-O-linked glycopeptides (Figure 2). It is selectively expressed on myelogenous leukemia cells. Fukuda, M., et al., ). Biol. Chem. 1986, 267, 12796. Saitoh, O., et al., Cancer Res. 1991, 57, 2854. Thus, in a specific embodiment, the present invention provides a synthetic route to pentapeptide 1, which is derived from the N-terminus of CD43 (Leukosialin) glycoprotein. Pallant, A., et al., Proc. Natl. Acad. Sci. USA 1989, 86, 1328. In particular, the invention provides a stereoselective preparation of α-O- linked (2,6)-ST glycosyl serine and threonine via a block approach. In addition, the present invention provides an O-linked glycopeptide incorporating such glycosyl units with clustered ST eρitopes (1,20). A broad range of carbohydrate domains are contemplated by the present invention. Special mention is made of the carbohydrate domains derived from the following cell surface epitopes and antigens: MBrl Epitope: Fucα1 -2Galβ1 -3GalNAcβ1 -3Galα1 -4Galβ1 -4Glu-0cer Truncated MBrl Epitope Pentasaccharide: Fucα1 -2Galβ1 -3GalNAcβ1 →3Galα1 -4Galβ1 Truncated MBrl Epitope Tetrasaccharide: Fucα1 →2Galβ1 -3GalNAcβ1 -3Galα1 SSEA-3 Antigen: 2Galβ1 -3GalNAcβ1 →3Galα1 -4Galβ1 Le y Epitope: Fucα1 -2Galβ1 →4(Fucα1 →3)GalNAcβ1 GM1 Epitope: Galβ1 →3GalNAcβ1 ~4Galβ1 -4(NeuAcα2-3)Glu-0cer Methods for preparing carbohydrate domains based on a solid-phase methodology have been disclosed in U.S. Serial Nos. 08/213,053 and 08/430,355, and in PCT International Application No. PCT/US96/10229, the contents of which are incorporated by reference. The present invention also provides a glycoconjugate having the structure: wherein m, n and p are integers between about 8 and about 20; wherein q is an integer between about 1 and about 8; wherein R v , R w , R x and R γ are independently hydrogen, optionally substituted linear or branched chain lower alkyl or optionally substituted phenyl; wherein R A , R B and R c are independently a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein R 0 is hydrogen, linear or branched chain lower alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR', NH 2 , NHCOR', F, CH 2 OH, CH 2 OR', an optionally substituted linear or branched chain lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R,„ R 12 , R 13 , R 14 and R 15 are each independently hydrogen, OH, OR'", NH 2 , NHCOR'", F, CH 2 OH, CH 2 OR'", or an optionally substituted linear or branched chain lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R 16 is hydrogen, COOH, COOR", CONHR", optionally substituted linear or branched chain lower alkyl or aryl group; wherein R'" is hydrogen, CHO, COOR' v , or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group; and wherein R" and R i are each independently hydrogen, or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group. In a certain embodiment, the invention provides a glycoconjugate wherein R v , R w , R x and R γ are methyl. In a certain other embodiment, the carbohydrate domains may be independently monosaccharides or disaccharides. In one embodiment, the invention provides a glycoconjugate wherein y and z are 0; wherein x is 1 ; and wherein R 3 is NHAc. In another embodiment, the invention provides a glycoconjugate wherein h is 0; wherein g and / are 1 ; wherein R 7 is OH; wherein R 0 is hydrogen; and wherein R 8 is hydroxymethyl. In yet another embodiment, m, n and p are 14; and wherein q is 3. In a preferred embodiment, each amino acyl residue of the glycoconjugate therein has an L-configuration. In a specific example, the carbohydrate domains of the glcyoconjugate are independently: In another example, the carbohydrate domains are independently Additionally, the carbohydrate domains are independently: The carbohydrate domains are also independently: The carbohydrate domains also are independently Also, the carbohydrate domains may be independently: The carbohydrate domains are also independently: The present invention provides a glycoconjugate having the structure: wherein the carrier is a protein; wherein the cross linker is a moiety derived from a cross linking reagent capable of conjugating a surface amine of the carrier and a thiol; wherein m, n and p are integers between about 8 and about 20; wherein / and q are independently integers between about 1 and about 8; wherein R v , R w , R x and R γ are independently hydrogen, optionally substituted linear or branched chain lower alkyl or optionally substituted phenyl; wherein R A , R B and R c are independently a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein R 0 is hydrogen, linear or branched chain lower alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR', NH 2 , NHCOR', F, CH 2 OH, CH 2 OR', an optionally substituted linear or branched chain lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R , R 12 , R 13 , R 14 and R 15 are each independently hydrogen, OH, OR'", NH 2 , NHCOR'", F, CH 2 OH, CH 2 OR'", or an optionally substituted linear or branched chain lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tr acyloxyalkyl, arylalkyi or aryl group; wherein R 16 is hydrogen, COOH, COOR", CONHR", optionally substituted linear or branched chain lower alkyl or aryl group; wherein R'" is hydrogen, CHO, COOR' v , or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group; and wherein R" and R' v are each independently hydrogen, or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group. Various proteins are contemplated as being suitable, including bovine serum albumin, KLH, and human serum albumin. Cross linkers suited to the invention are widely known in the art, including bromoacetic NHS ester, 6-(iodoacetamido)caproic acid NHS ester, maleimidoacetic acid NHS ester, maleimidobenzoic acid NHS ester, etc., In one embodiment, the glycoconjugate has the structure: In one embodiment, the invention provides the glycoconjugate wherein R v , R w , R x and R γ are methyl. In another embodiment, the invention provides the glycoconjugate wherein the carbohydrate domains are monosaccharides or disaccharides. In another embodiment, the invention provides the glycoconjugate wherein y and z are 0; wherein x is 1 ; and wherein R 3 is NHAc. In a further embodiment, the invention provides the glycoconjugate wherein h is 0; wherein g and / ' are 1 ; wherein R 7 is OH; wherein R 0 is hydrogen; wherein , n and p are 14; and wherein q is 3; and wherein R 8 is hydroxymethyl. In a certain embodiment, the invention provides the glycoconjugate as disclosed wherein the protein is BSA or KLH. In a preferred embodiment, each amino acyl residue of the glycoconjugate has an L-configuration. Specific examples of the glycoconjugate contain any of the following carbohydrate domains, which may be either the same or different in any embodiment. 24 The present invention further provides a pharmaceutical composition for treating cancer comprising a glycoconjugate as above disclosed and a pharmaceutically suitable carrier. The invention also provides a method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of a glycoconjugate disclosed above and a pharmaceutically suitable carrier. In a certain embodiment, the invention provides the method wherein the cancer is a solid tumor. Specifically, the method is applicable wherein the cancer is an epithelial cancer. Particularly effective is the application to treat prostate cancer. The invention also provides a method of inducing antibodies in a human subject, wherein the antibodies are capable of specifically binding with human tumor cells, which comprises administering to the subject an amount of the glycoconjugate disclosed above effective to induce the antibodies. In a certain embodiment, the invention provides the method wherein the carrier protein is bovine serum albumin, polylysine or KLH. In addition, the invention provides the related method of inducing antibodies which further comprises co-administering an immunological adjuvant. The adjuvant is preferably bacteria or liposomes. In particular, the adjuvant is Salmonella minnesota cells, baciile Calmette-Guerin or QS21. The antibodies induced are favorably selected from the group consisting of Tn, ST N , (2,3)ST, glycophorine, 3-Le y , 6-Le y , T(TF) and T antibodies. The invention further provides the method of inducing antibodies wherein the subject is in clinical remission or, where the subject has been treated by surgery, has limited unresected disease. The invention also provides a method of preventing recurrence of epithelial cancer in a subject which comprises vaccinating the subject with the glycoconjugate disclosed above which amount is effective to induce antibodies. The method may be practiced wherein the carrier protein is bovine serum albumin, polylysine or KLH. In addition, the invention provides the related method of preventing recurrence of epithelial cancer which further comprises co-administering an immunological adjuvant. Preferably, the adjuvant is bacteria or liposomes. Specifically, the preferred adjuvant is Salmonella minnesota cells, baciile Calmette-Guerin or QS21. The antibodies induced in the practice of the methods are selected from the group consisting of Tn, ST N , (2,3)ST, glycophorine, 3-Le y , 6- Le y , T(TF) and T antibodies. The present invention also provides a method of preparing a protected O- linked Le y glycoconjugate having the structure: wherein R is hydrogen, linear or branched chain lower alkyl, or optionally substituted aryl; R, is t-butyloxycarbonyl, fluorenylmethyleneoxycarbonyl, linear or branched chain lower alkyl or acyl, optionally substituted benzyl or aryl; R 2 is a linear or branched chain lower alkyl, or optionally substituted benzyl or aryl; and R 4 is hydrogen, linear or branched chain lower alkyl or acyl, optionally substituted aryl or benzyl, or optionally substituted aryl sulfonyl; which comprises coupling a tetrasaccharide sulfide having the structure: wherein R 3 is linear or branched chain lower alkyl or aryl; with an O-linked glycosyl amino acyl component having the structure: under suitable conditions to form the protected O-linked Le y glycoconjugate. In one embodiment of the invention, the tetrasaccharide sulfide shown above may be prepared by (a) halosulfonamidating a tetrasaccharide glycal having the structure: under suitable conditions to form a tetrasaccharide halosulfonamidate; and (b) treating the halosulfonamidate with a mercaptan and a suitable base to form the tetrasaccharide sulfide. In particular, the method may be practiced wherein the mercaptan is a linear or branched chain lower alkyl or an aryl; and the base is sodium hydride, lithium hydride, potassium hydride, lithium diethylamide, lithium diisopropylamide, sodium amide, or lithium hexamethyldisilazide. The invention also provides an O-linked glycoconjugate prepared by the method disclosed. In particular, the invention provides an O-linked glycopeptide having the structure: wherein R 4 is a linear or branched chain lower acyl; and wherein R is hydrogen or a linear or branched chain lower alkyl or aryl. Variations in the peptidic portion of the glycopeptide are within the scope the invention. In a specific embodiment, the invention provides the O- linked glycopeptide wherein R 4 is acetyl. The present invention provides a method of preparing a protected O-linked Le y glycoconjugate having the structure: wherein R is hydrogen, linear or branched chain lower alkyl, or optionally substituted aryl; R, is t-butyloxycarbonyl, fluorenylmethyleneoxycarbonyl, linear or branched chain lower alkyl or acyl, optionally substituted benzyl or aryl; and R 2 is a linear or branched chain lower alkyl, or optionally substituted benzyl or aryl; which comprises coupling a tetrasaccharide azidoimidate having the structure: with an O-linked glycosyl amino acyl component having the structure: under suitable conditions to form the protected O-linked Le y glycoconjugate. The tetrasaccharide azidoimidate is favorably prepared by (a) treating tetrasaccharide azidonitrate having the structure: under suitable conditions to form an azido alcohol; and (b) reacting the azido alcohol with an imidoacylating reagent under suitable conditions to form the azidoimidate. The tetrasaccharide azido nitrate may be prepared by (a) converting a tetrasaccharide glycal having the structure: under suitable conditions to a peracetylated tetrasaccharide glycal having the structure: and (b) azidonitrating the glycal formed in step (a) under suitable conditions to form the tetrasaccharide azido nitrate. Step (b) is favorably effected using cerium ammonium nitrate in the presence of an azide salt selected from the group consisting of sodium azide, lithium azide, potassium azide, tetramethylammonium azide and tetraethylammonium azide. In addition, the invention provides an O-linked glycoconjugate prepared as shown above. Once the carbohydrate domains covalently linked to O-bearing aminoacyl side chains are prepared, the glycoconjugates of the subject invention may be prepared using either solution-phase or solid-phase synthesis protocols, both of which are well-known in the art for synthesizing simple peptides. Among other methods, a widely used solution phase peptide synthesis method useful in the present invention uses FMOC (or a related carbamate) as the protecting group for the α-amino functional group; ammonia, a primary or secondary amine (such as morpholine) to remove the FMOC protecting group and a substituted carbodiimide (such as N,N'-dicyclohexyl- or -diisopropylcarbodiimide) as the coupling agent for the C to N synthesis of peptides or peptide derivatives in a proper organic solvent. Solution-phase and solid phase synthesis of O-linked glycoconjugates in the N to C direction is also within the scope of the subject invention. For solid-phase synthesis, several different resin supports have been adopted as standards in the field. Besides the original chloromethylated polystyrene of Merrifield, other types of resin have been widely used to prepare peptide amides and acids, including benzhydrylamine and hydroxymethyl resins (Stewart, So/ cf Phase Peptide Synthesis, Pierce Chemical Co., 1984, Rockford, IL; Pietta, et al., j. Chem. Soc. D., 1970, 650-651 ; Orlowski, et al, ). Org. Chem., 1976, 50, 3701-5; Matsueda et al, Peptides, 1981, 2, 45-50; and Tarn, \. Org. Chem., 1985, 50, 5291-8) and a resin consisting of a functionalized polystyrene-grafted polymer substrate (U.S. Patent No. 5,258,454). These solid phases are acid labile (Albericio, et al, Int. .. Peptide Research. 1987, 30, 206-216). Another acid labile resin readily applicable in practicing the present invention uses a trialkoxydi-phenylmethylester moiety in conjunction with FMOC-protected amino acids (Rink, Tetrahedron Letters, 1987, 28, 3787-90; U.S. Pat. No. 4,859,736; and U.S. Pat. No. 5,004,781 ). The peptide is eventually released by cleavage with trifluoroacetic acid. Adaptation of the methods of the invention for a particular resin protocol, whether based on acid-labile or base-sensitive N-protecting groups, includes the selection of compatible protecting groups, and is within the skill of the ordinary worker in the chemical arts. The glycoconjugates prepared as disclosed herein are useful in the treatment and prevention of various forms of cancer. Thus, the invention provides a method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of any of the α-O-linked glycoconjugates disclosed herein, optionally in combination with a pharmaceutically suitable carrier. The method may be applied where the cancer is a solid tumor or an epithelial tumor, or leukemia. In particular, the method is applicable where the cancer is breast cancer, where the relevant epitope may be MBrl . The subject invention also provides a pharmaceutical composition for treating cancer comprising any of the α-O-linked glycoconjugates disclosed hereinabove, as an active ingredient, optionally though typically in combination with a pharmaceutically suitable carrier. The pharmaceutical compositions of the present invention may further comprise other therapeutically active ingredients. The subject invention further provides a method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of any of the α-O-linked glycoconjugates disclosed hereinabove and a pharmaceutically suitable carrier. The compounds taught above which are related to α-O-linked glycoconjugates are useful in the treatment of cancer, both in vivo and in vitro. The ability of these compounds to inhibit cancer cell propagation and reduce tumor size in tissue culture will show that the compounds are useful to treat, prevent or ameliorate cancer in subjects suffering therefrom. In addition, the glycoconjugates prepared by processes disclosed herein are antigens useful in adjuvant therapies as vaccines capable of inducing antibodies immunoreactive with various epithelial tumor and leukemia cells. Such adjuvant therapies may reduce the rate of recurrence of epithelial cancers and leukemia, and increase survival rates after surgery. Clinical trials on patients surgically treated for cancer who are then treated with vaccines prepared from a cell surface differentiation antigen found in patients lacking the antibody prior to immunization, a highly significant increase in disease-free interval may be observed. Cf. P.O. Livingston, et al., . Clin. Oncol., 1994, 12, 1036. The magnitude of the therapeutic dose of the compounds of the invention will vary with the nature and severity of the condition to be treated and with the particular compound and its route of administration. In general, the daily dose range for anticancer activity lies in the range of 0.001 to 25 mg/ kg of body weight in a mammal, preferably 0.001 to 10 mg/kg, and most preferably 0.001 to 1.0 mg/kg, in single or multiple doses. In unusual cases, it may be necessary to administer doses above 25 mg/kg. Any suitable route of administration may be employed for providing a mammal, especially a human, with an effective dosage of a compound disclosed herein. For example, oral, rectal, topical, parenteral, ocular, pulmonary, nasal, etc., routes may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, aerosols, etc. The compositions include compositions suitable for oral, rectal, topical (including transdermal devices, aerosols, creams, ointments, lotions and dusting powders), parenteral (including subcutaneous, intramuscular and intravenous), ocular (ophthalmic), pulmonary (nasal or buccal inhalation) or nasal administration. Although the most suitable route in any given case will depend largely on the nature and severity of the condition being treated and on the nature of the active ingredient. They may be conveniently presented in unit dosage form and prepared by any of the methods well known in the art of pharmacy. In preparing oral dosage forms, any of the unusual pharmaceutical media may be used, such as water, glycols, oils, alcohols, flavoring agents, preservatives, coloring agents, and the like in the case of oral liquid preparations (e.g., suspensions, elixers and solutions); or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, disintegrating agents, etc., in the case of oral solid preparations are preferred over liquid oral preparations such as powders, capsules and tablets. If desired, capsules may be coated by standard aqueous or non-aqueous techniques. In addition to the dosage forms described above, the compounds of the invention may be administered by controlled release means and devices. Pharmaceutical compositions of the present invention suitable for oral administration may be prepared as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient in powder or granular form or as a solution or suspension in an aqueous or nonaqueous liquid or in an oil-in-water or water-m- oil emulsion. Such compositions may be prepared by any of the methods known in the art of pharmacy. In general compositions are prepared by uniformly and intimately admixing the active ingredient with liquid carriers, finely divided solid carriers, or both and then, if necessary, shaping the product into the desired form. For example, a tablet may be prepared by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free- flowing form such as powder or granule optionally mixed with a binder, lubricant, inert diluent or surface active or dispersing agent. Molded tablets may be made by molding in a suitable machine, a mixture of the powdered compound moistened with an inert liquid diluent. The present invention will be better understood from the Experimental Details which follow. However, one skilled in the art will readily appreciate that the specific methods and results discussed are merely illustrative of the invention as described in the claims which follow thereafter. It will be understood that the processes of the present invention for preparing α-O- nked glycoconjugates encompass the use of various alternate protecting groups known in the art. Those protecting groups used in the disclosure including the Examples below are merely illustrative. Experimental Details: General Procedures All air- and moisture-sensitive reactions were performed in a flame-dried apparatus under an argon atmosphere unless otherwise noted. Air-sensitive liquids and solutions were transferred via syringe or canula. Wherever possible, reactions were monitored by thin-layer chromatography (TLC). Gross solvent removal was performed in vacuum under aspirator vacuum on a Buchi rotary evaporator, and trace solvent was removed on a high vacuum pump at 0.1-0.5 mmHg. Melting points (mp) were uncorrected and performed in soft glass capillary tubes using an Electrothermal series IA9100 digital melting point apparatus. Infrared spectra (IR) were recorded using a Perkin-Elmer 1600 series Fourier-Transform instrument. Samples were prepared as neat films on NaCI plates unless otherwise noted. Absorption bands are reported in waven,umbers (cm 1 ). Only relevant, assignable bands are reported. Proton nuclear magnetic resonance (' H NMR) spectra were determined using a Bruker AMX-400 spectrometer at 400 MHz. Chemical shifts are reported in parts per million (ppm) downfield from tetramethylsilane (TMS; δ = 0 ppm) using residual CHCI 3 as a lock reference (6 = 7.25 ppm). Multiplicities are abbreviated in the usual fashion: s = singlet; d = doublet; t=triplet; q = quartet; m = multiplet; br = broad. Carbon nuclear magnetic resonance ( n C NMR) spectra were performed on a Bruker AMX-400 spectrometer at 100 MHz with composite pulse decoupling. Samples were prepared as with 'H NMR spectra, and chemical shifts are reported relative to TMS (0 ppm) ; residual CHC1 3 was used as an internal reference (6 = 77.0 ppm). All high resolution mass spectral (HRMS) analyses were determined by electron impact ionization (El) on a JEOL JMS-DX 303HF mass spectrometer with perfluorokerosene (PFK) as an internal standard. Low resolution mass spectra (MS) were deter-mined by either electron impact ionization (El) or chemical ionization (Cl) using the indicated carrier gas (ammonia or methane) on a Delsi-Nermag R-10-10 mass spectrometer. For gas chromatography/mass spectra (GCMS), a DB-5 fused capillary column (30 m, 0.25mm thickness) was used with helium as the carrier gas. Typical conditions used a temperature program from 60-250°C at 40°C/min. Thin layer chromatography (TLC) was performed using precoated glass plates (silica gel 60, 0.25 mm thickness). Visualization was done by illumination with a 254 nm UV lamp, or by immersion in anisaldehyde stain (9.2 mL p-anisaldehyde in 3.5 mL acetic acid, 12.5 mL cone, sulfuric acid and 338 mL 95.% ethanol (EtOH)) and heating to colorization. Flash silica gel chromatography was carried out according to the standard protocol. Unless otherwise noted, all solvents and reagents were commercial grade and were used as received, except as indicated hereinbelow, where solvents were distilled under argon using the drying methods listed in parentheses: CH 2 CI 2 (CaH 2 ); benzene (CaH 2 ); THF (Na/ketyl); Et 2 0 (Na/ketyl); diisopropylamine (CaH 2 ). Abbreviations TLC thin layer chromatography EtOAc ethyl acetate TIPS triisopropylsilyl PMB p-methoxybenzyl Bn benzyl Ac acetate hex hexane THF tetrahydrofuran coll , collidine LiHMDS lithium hexamethyldisilazide DMF N,N-dimethylformamide DMAP 2-dimethylaminopyridine DDQ 2,3-dichloro-5,6-dicyano-1 ,4-benzoquinone TBAF tetra-n-butylammonium fluoride M.S. molecular sieves r.t. room temperature r.b. round bottom flask EXAMPLE 1 2,6-Di-0-acetyl-3,4-0-carbonyl-β-D-galactopyranosyl-{1-3)-6-0-(triisopropylsilyl>-4-0-acetyl- galactal (3). Galactal 2 (1.959g, 9.89 mmol, 1.2 eq.) was dissolved in 100 mL of anhydrous CH 2 CI 2 and cooled to 0°C. Solution of dimethyldioxirane (200 mL of ca 0.06M solution in acetone) was added via cannula to the reaction flask. After 1 hr the starting material was consumed as judged by TLC. Solvent was removed with a stream of N 2 and the crude epoxide was dried in vacuo for 1 hr at room temperature. The crude residue (single spot by TLC) was taken up in 33mL of THF and 6-O-triisopropyl-galactal acceptor (2.50g, 8.24 mmol) in 20 mL THF was added. The resulting mixture was cooled to -78°C and ZnCI 2 (9.8mL of 1M solution in ether) was added dropwise. The reaction was slowly warmed up to rt and stirred overnight. The mixture was diluted with EtOAc and washed with sat. sodium bicarbonate, then with brine and finally dried over MgS0 4 . After evaporation of the solvent the crude material was purified by flash chromatography (40-45-50-60% EtOAc/ hexane) to yield pure product which was immediately acetylated. 3.36g was dissolved in 50 mL of dry CH 2 CI 2 , triethylamine (19.2 mL), cat amount of DMAP (ca 20mg) were added and the solution was cooled to OC. Acetic anhydride (9.9 mL) was added dropwise at 0 C C. The reaction was stirred at rt overnight. The solvent was removed in vacuo and the crude material was chromatographed (50% EtOAc/ hexane) to give glycal 3 (3.3g, 75%): Η NMR (500MHz, CDCI 3 ) 6 6.42 (d, J - 6.3 Hz, 1 H, H-1 , glycal), 4.35 (!_ AB, dd, J = 6.8 Hz, 11.5 Hz, 1 H, H- 6'a), 4.28 (1/2AB, dd, J = 6.1 , 1 1.5 Hz, 1 H, H-6'b). EXAMPLE 2 2,6-Di-0-acetyl-3,4-0-carbonyl-β-D-galactopyranosyl-(1-3)-4-0-acetyl-galactal (4). Compound 3 (1.5 g, 2.43 mmol) was dissolved in 24 mL of THF and cooled to 0°C. A mixture of TBAF (5.8 mL, 5.83 mmol, 2.4 eq.) and acetic acid (336 mL, 2.4 eq.) was added to the substrate at 0°C. The reaction was stirred at 30°C for 5 hrs. The reaction mixture was diluted with ethyl acetate and quenched with sat sodium bicarbonate. Organic phase was washed with sat sodium bicarbonate, brine and subsequently dried over magnesium sulphate. The crude product was purified by chromatography (80-85-90% EtOAc/ hexane) to yield compound 4 (0.9 g, 80%): 'H NMR (500MHz, CDCI 3 ) 6 6.38 (dd, J = 1.8, 6.3 Hz, 1 H, H-1 , glycal), 5.39 (m, 1 H, H-4), 2.22 (s, 3H, acetate), 2.16 (s, 3H, acetate), 2.13 (s, 3H, acetate). EXAMPLE 3 [(Methyl 5-acetamido-4,7,8,9-tetra-0-acetyl-3,5-dideoxy-0-glycero-α-D-galacto-2- nonulopyranosylonateH2-6)]-(2,6-di-0-acetyl-3,4-0<arbonyl-β-D-galactopyranosylH1-3)-4- O-acetyl-galactal. (6). A flame dried flask was charged with sialyl phosphite donor 5 (69 mg, 0.1 1 mmol, 1.3 eq.) and acceptor 4 (40 mg, 0.085 mmol) in the dry box (Argon atmosphere). The mixture was dissolved in 0.6 mL of dry THF. 0.6 mL of dry toluene was added and the solution was slowly cooled to -60°C to avoid precipitation. Trimethylsilyl triflate (2.4 μL, 0.11 eq.) was added and the mixture was stirred at -45°C. The reaction was quenched at - 45°C after 2 hrs (completion judged by TLC) with 2 mL of sat. sodium bicarbonate, warmed until water melted and the mixture was poured into an excess of ethyl acetate. Organic layer was washed with sat. sodium bicarbonate and dried over anhydrous sodium sulphate. Η NMR of the crude material revealed a 4:1 ratio of α:β isomers (66.4 mg, 84%). The mixture was separated by flash chromatography on silica gel (2-2.5-3-3.5-4% MeOH/CH 2 CI 2 ) to yield compound 6 (50 mg, 63 % yield): 'H NMR (500MHz, CDCI 3 ) 6 6.42 (d, J = 6.2 Hz, 1 H), 5.37 (m, 1 H), 5.32 - 5.29 (m, 4H), 5.26 - 5.24 (m, 1 H), 5.12 - 5.10 (m, 2H), 4.98 (d, J - 3.5 Hz, 1 H), 4.92 - 4.85 (m, 1 H), 4.83 - 4.80 (m, 3H), 4.54 (m, 1 H), 4.45 (dd, J = 3.0, 13.5 Hz, 1 H), 4.33 - 4.20 (m, 3H), 4.22 - 4.02 (m, 7H), 3.96 (dd, J = 7.6, 10.9 Hz, 1 H, H-2), 2.59 (dd, J = 4.6, 12.9 Hz, 1 H, H-2e NeuNAc), 2.30 (dd, J - 12.9 Hz, 1 H, H-2ax NeuNAc), 2.16, 2.14, 2.13, 2.12, 2.06, 2.03, 2.02 (s, 7x3H, acetates), 1.88 (s, 3H, C/-/3CONH); FTIR (neat) 2959.2 (C-H), 1816.5, 1745.0 (C- O), 1683.6, 1662.4 (glycal C=C), 1370.6, 1226.9, 1038.7; HRMS (El) calc. for C39H51 N025K (M + K) 972.2386, found 972.2407. EXAMPLE 4 α/β Mixture of azidonitrates 7. Compound 6 (370 mg, 0.396 mmol) was dissolved in 2.2 mL of dry acetonitrile and the solution was cooled to -20°C. Sodium azide (NaN 3 , 38.6 mg, 0.594, 1.5 eq.) and cerium ammonium nitrate (CAN, 651.3, 1.188 mmol, 3eq.) were added and the mixture was vigorously stirred at -15°C for 12 hrs. The heterogeneous mixture was diluted with ethyl acetate, washed twice with ice cold water and dried over sodium sulphate to provide 400 mg of the crude product. Purification by flash chromatography provided mixture 7 (246 mg, 60 % yield): Η NMR (400MHz, CDCI 3 ) 6.35 (d, J = 4.2 Hz, 1 H, H-1 , α- nitrate), 3.79 (s, 3H, methyl ester), 3.41 (dd, J = 4.7, 1 1.0, 1 H, H-2), 2.54 (dd, J = 4.6, 12.8, H-2eq NeuNAc); FTIR (neat) 21 1 7.4 (N3), 1733.9 (C = 0); MS (El) calc. 1037.8, found 1038.4 (M + H). EXAMPLE 5 α-Azidobromide 8. A solution of the compound 7 (150 mg, 0.145 mmol) in 0.6 mL of dry acetonitrile was mixed with lithium bromide (62.7 mg, 0.725 mmol, 5eq.) and stirred at rt for 3hrs in the dark. The heterogeneous mixture was diluted with dichloromethane and the solution was washed twice with water, dried over magnesium sulphate and the solvent was evaporated without heating. After flash chromatography (5% MeOH, CH 2 CI 2 ) α-bromide 8 (120 mg, 75% yield) was isolated and stored under an argon atmosphere at -80°C: 'H NMR (500MHz, CDCI 3 ) 6 6.54 (d, J = 3.7 Hz, 1 H, H-1 ), 3.40 (dd, J = 4.5, 10.8 Hz, 1 H, H-2), 2.57 (dd, J = 4.5, 12.9, 1 H, H-2eq NeuNAc), 2.20, 2.15, 2.14, 2.12, 2.04, 2.02 (singlets, each 3H, acetates), 1 .87 (s, 3H, CH3CONH); MS (El) calc. for C39H51 N4Br025 1055.7, found 1057.4 (M + H). EXAMPLE 6 Azido-trichloroacetamidate 9. Compound 7 (600mg, 0.578 mmol) was dissolved in 3.6 mL of acetonitrile and the resulting solution was treated with thiophenol (180 μL) and diisopropylethylamine (100μL). After 10 minutes the solvent was removed with a stream of nitrogen. The crude material was purified by chromatography (2-2.5-3-3.5% MeOH/CH 2 CI 2 ) to provide 472 mg (82%) of intermediate hemiacetal. 60 mg (0.06mmol) of this intermediate was taken up in 200 mL of CH 2 CI 2 and treated with trichloroacetonitrile (60 μL) and 60 mg potassium carbonate. After 6 hrs the mixture is diluted with CH 2 CI 2 , solution is removed with a pipette and the excess K 2 C0 3 was washed three times with CH 2 CI 2 . After evaporation of solvent the crude was purified by flash chromatography (5%MeOH/CH 2 CI 2 ) to provide 9 (53.2 mg, 64% yield for two steps, 1 :1 mixture of α/β anomers). The anomers can be separated by flash chromatography using a graded series of solvent systems (85-90-95-100% EtOAc hexane). EXAMPLE 7 Preparation of glycosyl-L-threonine 13 by AgCI0 4 -promoted glycosidation with glycosyl bromide 8. A flame dried flask is charged with silver perchlorate (27.3 mg, 2 eq), 115 mg of 4A molecular sieves and N-FMOC-L-threonine benzyl ester (37.3 mg, 0.086 mmol, 1.2 eq) in the dry box. 0.72 mL of CH 2 CI 2 was added to the flask and the mixture was stirred at rt for 10 minutes. , Donor 8 (76 mg, 0.072 mmol) in 460 μL of CH 2 CI 2 was added slowly over 40 minutes. The reaction was stirred under argon atmosphere at rt for two hours. The mixture was then diluted with CH 2 CI 2 and filtered through celite. The precipitate was thoroughly washed with CH 2 CI 2 , the filtrate was evaporated and the crude material was purified on a silica gel column (1-1.5-2-2.5% MeOH/CH 2 CI 2 ) to provide 13 (74mg, 74% yield). The undesired β-anomer was not detected by Η NMR and HPLC analysis of the crude material. 13: Η NMR (500MHz, CDCI 3 ) 6 7.77 (d, J = 7.5 Hz, 2H), 7.63 (d, J = 7.2 Hz, 2H), 7.40 - 7.25 (m, 8H), 5.72 (d, 9.2 Hz, 1 H), 5.46 (s, 1 H), 5.33 (m, 1 H), 5.29 (d, J = 8.2 Hz, 1 H), 5.23 (s, 2H), 5.1 1 - 5.04 (m, 3H), 4.87 - 4.71 (m, 4H), 4.43 - 4.39 (m, 3H), 4.33 - 4.25 (m, 4H), 4.09 - 3.97 (m, 6H), 3.79 (s, 3H, methyl ester), 3.66 (dd, J = 3.7, 10.6 Hz, 1 H, H-3), 3.38 (dd, J = 3.0, 10.7 Hz, 1 H, H-2), 2.52 (dd, J = 4.3, 12.7, 1 H, H-2eq NeuNAc), 2.20, 2.13, 2.1 1 , 2.10, 2.04, 2.03, 2.02 (singlets, 3H, acetates), 1.87 (s, 3H, CH3CON H), 1.35 (d, J = 6.15 Hz, Thr-CH 3 ); FTIR (neat) 21 10.3 (N3), 1 748.7 (C = 0), 1223.9, 1043.6; HRMS (El) calc. for C65H75N5O30K (M + K) 1444.4130, found 1444.4155. EXAMPLE 8 Glycosyl-L-serine 12. BF 3 OEt 2 promoted glycosydation with trichloroacetamidate 9: A flame dried flask is charged with donor 9 (50 mg, 0.044 mmol), 80 mg of 4A molecular sieves and N-FMOC-L- serine benzyl ester (27.5 mg, 0.066 mmol) in the dry box. 0.6 mL of THF was added to the flask and the mixture was cooled to -30°C. BF 3 OEt 2 (2.8 mL, 0.022 mmol, 0.5 eq.) was added and the reaction was stirred under argon atmosphere. During three hours the mixture was warmed to -10°C and then diluted with EtOAc and washed with sat sodium bicarbonate while still cold. The crude material was purified on silica gel column (2-2.5-3% MeOH/ CH 2 CI 2 ) to provide 12 (40 mg, 66% yield) as a 4:1 mixture of α:β isomers. The pure α-anomer was separated by flash chromatography (80-85-90-100% EtOAc/ hexane). EXAMPLE 9 Glycosyl-L-threonine (15). Compound 13 (47 mg, 33.42 μmol) was treated with thiolacetic acid (3 mL, distilled three times) for 27 hrs at rt. Thiolacetic acid was removed with a stream of nitrogen, followed by toluene evaporation (four times). The crude product was purified by flash chromatography (1.5-2-2.5-3-3.5% MeOH/CH 2 CI 2 ) to yield 37 mg (78%) of an intermediated which was immediately dissolved in 7.6 mL of methanol and 0.5 mL of water. After purging the system with argon 6.5 mg of palladium catalyst (10% Pd-C) was added and hydrogen balloon was attached. After 8 hrs hydrogen was removed by argon atmosphere, the catalyst was removed by filtration through filter paper and the crude material was obtained upon removal of solvent. Flash Chromatography (10% MeOH/CH 2 CI 2 ) provided pure compound 15 ( 36 mg, 78%): 'H NMR (500MHz, CDCI 3 ) mixture of rotamers, characteristic peaks 6 3.80 (s, 3H, methyl ester), 3.41 (m, 1 H, H-2), 2.53 (m, 1 H, H-2e NeuNAc)), 1.45 (d, J - 5.1 Hz, Thr-CH 3 ), 1.35 (d, J = 5.8 Hz, Thr- CH3); FTIR (neat) 1818.2, 1 747.2 (C-O), 1371.1 , 1225.6, 1045.0; HRMS (El) calc. for C60H73N3O31 K (M + K) 1370.3870, found 1370.391 1. EXAMPLE 10 Glycosyl-L-serine (14). The compound 14 was prepared in 80% yield from 12 following the same procedure as for 15. EXAMPLE 11 General procedure for peptide coupling: Glycosyl amino acid 14 or 15 (1 eq) and the peptide with a free amino group (1.2 eq) were dissolved in CH 2 CI 2 (22 mL/1 mmol). The solution was cooled to 0°C and IIDQ (1.15 -1.3 eq.) is added (1 mg in ca 20mL CH 2 CI 2 ). The reaction was then stirred at rt for 8 hrs. The mixture was directly added to the silica gel column. EXAMPLE 12 General procedure for FMOC deprotection: A substrate (1 mmol in 36 mL DMF) was dissolved in anhydrous DMF followed by addition of KF (10eq) and 18-crown-6 ether (catalytic amount). The mixture was then stirred for 48 hrs at rt. Evaporation of DMF in vacuo was followed by flash chromatography on silica gel. EXAMPLE 13 Glycopeptide 16. 'H NMR (500MHz, CDCI 3 ) 6 3.45 - 3.30 (m, 3x1 H, H-2), 3.74 (s, 3H, methyl ester), 2.58 - 2.49 (m, 3x1 H, H-2eq NeuNAc); FTIR (neat) 2961.7, 1819.2, 1746.5, 1663.5, 1370.5, 1225.7, 1042.5; MS (El) calc. 3760, found 1903.8 / doubly charged = 3806 (M + 2Na). EXAMPLE 14 Glycopeptide 1. ' HNMR (500 MHz, D 2 0) d 4.73 (m, 2H, 2xH-1 ), 4.70 (d, 1 H, H-1), 4.64 (m, 3H, 3xH-1 '), 4.26 -4.20 (m, 5H), 4.12 - 4.00 (m, 7H), 3.95 - 3.82 (7H), 3.77 - 3.27 (m, 51 H), 2.55 - 2.51 (m, 3H, 3xH-2eq NeuNAc), 1.84 - 1.82 (m, 21 H, CH3CONH), 1.52 - 1.45 (m, 3H, H-2ax NeuNAc), 1.20 (d, J = 7.2 Hz, 3H), 1.18 (d, J = 6.6 Hz, 3H), 1.12 (d, J = 6.2 Hz, 3H), 0.71 (d, J = 6.6 Hz, 6H, val); 13C NMR (500MHz, D20) anomeric carbons: 105.06, 105.01 , 100.60, 100.57, 100.53, 100.1 1 , 9g.52, 98.70; MS (FAB) C96H157N1 1064 2489 (M + H); MS(MALDI) 2497. EXAMPLE 15 Glycopeptide 19. MS (El) calc. for C178H249N15094Na2 4146 (M + 2Na), found 4147, negative ionization mode confirmed the correct mass; MALDI (Matrix Assisted Laser Desorption Ionization) provided masses 4131 , 4163. EXAMPLE 16 Glycopeptide 20: MS (FAB) C1 19H193N 15O70N 2975 (M + Na) EXAMPLE 17 Preparation of azidonitrates 4': To a solution of protected galactal 3' (4.14 g, 12.1 mmol) in 60 ml of anhydrous CH 3 CN at -20 °C was added a mixture of NaN 3 (1.18 g, 18.1 mmol) and CAN (19.8 g, 36.2 mmol). The reaction mixture was vigorously stirred at -20 °C for overnight. Then the reaction mixture was diluted with diethyl ether, and washed with cold water and brine subsequently. Finally, the solution was dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel. A mixture of α- and β-isomers (4') (2.1 7 g, 40% yield) was obtained. The ratio of α-isomer and β-isomer was almost 1 :1 based on Η NMR. 4a': [α] D 20 94.5°(c 1.14, CHCI 3 ); FT-IR (film) 2940, 2862, 2106, 1661 , 1460, 1381 , 1278 cm "1 ; ' H NMR (300 MHz, CDCI 3 ) 6 6.34 (d, J = 3.9Hz, 1 H), 4.34(m, 2H), 4.21 (t, J = 6.4Hz, 1 H), 3.95 (dd, J = 9.6, 7.2Hz, 1 H), 3.85 (dd, J = 9.6, 6.4Hz, 1 H), 3.78 (m, 1 H), 1.52 (s, 3H), 1.35 (s, 3H), 1.04 (m, 21 H); 13 C NMR (75 MHz, CDCI j ) 6 1 10.29, 97.02, 73.36, 71.89, 71.23, 61.95, 59.57, 28.18, 25.96, 17.86, 1 1.91 ; HRMS(FAB) calc. for C 18 H 34 N 4 0 7 SiK [M + K + ] 485.1833, found 485.1821. 4b': [α] D 20 27.9° (c 1.28, CHCI 3 ); FT-IR (film) 2940, 2862, 2106, 1666, 1459, 1376, 1283 crrr 1 ; 'H NMR (300 MHz, CDC I3 ) 6 5.50 (d, J = 8.9Hz, 1 H), 4.30 (dd, J =4.3, 1.5Hz, 1 H), 4.15 (dd, J = 6.2, 4.3Hz, 1 H), 3.89-4.03 (m, 3H), 3.56 (dd, J = 8.9, 7.3Hz, I H), 1.58 (s, 3H), 1.38 (s, 3H), 1.08 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 1 10.90, 98.09, 77.53, 74.58, 71.99, 61.82, 61.68, 28.06, 25.97, 1 7.85, 1 1.89; HRMS (FAB) calc. for C 18 H 34 N 4 0 7 SiK [M + K + ] 485.1833, found 485.1857. EXAMPLE 18 Preparation of trichloroacetimidates 5a' and 5b': To a solution of a mixture of azidonitrates (4') (1.36 g, 3.04 mmol) in 10 ml of anhydrous CH 3 CN at 0 °C were slowly added Et(/ ' -Pr) 2 N (0.53 mj, 3.05 mmol) and PhSH (0.94 ml, 9.13 mmol) subsequently. The reaction mixture was stirred at 0 °C for 1 hour, then the solvent was evaporated at room temperature in vacuo. The residue was separated by chromatography on silica gel to give the hemiacetal (1.22 g, 99.8% yield). To a solution of this hemiacetal (603 mg, 1.50 mmol) in 15 ml of anhydrous CH 2 CI 2 at 0°C were added K 2 C0 3 (1.04 g, 7.50 mmol) and CCI 3 CN (1.50 ml, 15.02 mmol). The reaction mixture was stirred from 0°C to room temperature for 5 hours. The suspension was filtered through a pad of celite and washed with CH 2 CI 2 . The filtrate was evaporated and the residue was separated by chromatography on silica gel to give_α-trichloroacetimidate 5a' (118 mg, 14% yield), β-trichloroacetimidate 5b' (572 mg, 70% yield) and recovered hemiacetal (72 mg). 5a': [α] D 20 84.0° (c 1.02, CHCl 3 ); FT-IR (film) 2942, 2867, 21 1 1 , 1675, 1461 , 1381 , 1244 cm 1 ; Η NMR (300 MHz, CDC, 3 ) 6 8.69 (s, 1 H), 6.29 (d, J = 3.3Hz, 1 H), 4.47 (dd, J =8.0, 5.3Hz, 1 H), 4.39 (dd, J = 5.3, 2.4Hz, 1 H), 4.25 (m, 1 H), 3.97 (dd, J = 9.5, 7.8Hz, I H), 3.87 (dd, J = 9.5, 6.0Hz, 1 H), 3.67 (dd, J = 8.0, 3.3Hz, 1 H), 1.53 (s, 3H), 1.36 (s, 3H), 1.04 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 160.67, 109.98, 94.72, 77.20, 73.35, 72.1 1, 70.83, 62.01, 60.80, 28.29, 26.09, 17.88, 1 1.88; HRMS (FAB) calc. for C 20 H 35 N 4 O 5 SiKCI 3 [M + K + ] 583.1080, found 583.1071. 5b': [α] D 20 30.6° (c 1.12, CHCI 3 ); FT-IR (film) 2941 , 21 10, 1677, 1219 cπr 1 ; Η NMR (300 MHz, CDC, 3 )_δ 8.71 (s, 1 H), 5.57 (d, J - 9.0Hz, 1 H), 4.27 (d, J = 5.2Hz, 1 H), 3.95-4.02 (m, 4H), 3.63 (t, J = 9.0Hz, 1 H). 1.57 (s, 3H), 1.34 (s, 3H), 1.04 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 160.94, 1 10.55, 96.47, 77.20, 74.58, 72.21 , 64.84, 61.89, 28.29, 26.07, 17.87, 1 1.90; HRMS (FAB) calc. for C 20 H 35 N 4 O 5 SiKCI 3 [M + K + ] 583.1080, found 583.1073. EXAMPLE 19 Preparation of glycosyl fluorides 6a' and 6b': To a solution of the hemiacetal prepared previously (68.0 mg, 0.169 mmol) in 3 ml of anhydrous CH 2 CI 2 at 0 °C was added DAST (134 ml, 1.02 mmol) slowly. The reaction mixture was stirred at 0 °C for 1 hour. Then the mixture was diluted with EtOAc, washed with sat. NaHC0 3 and brine subsequently. Finally, the solution was dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give α-fluoride 6a' (30.2 mg, 44% yield) and β-fluoride 6b' (33.7 mg, 49% yield). 6a': [α] D 20 689.5° (c 1.47, CHCI 3 ); FT-IR (film) 2944, 2867, 21 15, 1462, 1381 cm '; Η NMR (300 MHz, CDC I3 ) 6 5.59 (dd, J = 53.0, 2.6Hz, 1 H), 4.34-4.40 (m, 2H), 4.26 (m, 1 H), 3.96 (t, J = 9.3Hz, 1 H), 3.88 (dd, J = 9.3, 6.0Hz, 1 H), 3.48 (ddd, J = 25.5, 7.0, 2.6Hz, 1 H), 1.50 (s, 3H), 1.34 (s, 3H), 1.05 (m, 21 H); ,3 C NMR (75 MHz, CDCI 3 ) 6 1 10.03, 107.45, 104.46, 77.21 , 76.38, 73.21 , 71.79, 70.48, 61.88, 61.23, 60.91 , 28.17, 26.03, 17.09, 11.92; HRMS (FAB) calc. for C 18 H 35 N 3 0 4 SiF [M + H + ] 404.2378, found 404.2369. 6b': [α] D 2 <° 153.8° (c 1.65, CHCI 3 ); FT-IR (film) 2943, 2867, 21 16, 1456, 1382, 1246 cm "1 ; 'H NMR (300 MHz, CDCI 3 ) 6 5.05 (dd, J = 52.6, 7.4Hz, 1 H), 4.27 (dt, J - 5.5, 2.0Hz, 1 H), 3.89- 4.05 (m, 4H), 3.70 (dt, J — 12.3, 5.1 Hz, 1 H), 1.53 (s, 3H), 1.32 (s, 3H), 1.04 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 1 10.64, 109.09, 106.24, 76.27, 76.16, 73.42, 71.63, 64.80, 64.52, 61.77, 27.80, 25.78, 1 7.03, 1 1.86; HRMS (FAB) calc. for C lβ H 35 N 3 0 4 SiF [M + H + ] 404.2378, found 404.2373. EXAMPLE 20 Coupling of β-trichloroacetimidate 5b' with protected serine derivative 7': Synthesis of 9a' and 9b': To a suspension of β-trichloroacetimidate 5b' (52.3 mg, 0.096 mmol), serine derivative 7' (44.0 mg, 0.105 mmol) and 200 mg 4λ molecular sieve in a mixture of 2 ml of anhydrous CH 2 Cl 2 and 2 ml of anhydrous hexane at -78 °C was added a solution of TMSOTf (1.91 μl, 0.01 mmol) in 36 μl of CH 2 CI 2 . The reaction mixture was stirred at -78 °C for a half hour, then warmed up to room temperature for 3 hours. The reaction was quenched by Et 3 N. The suspension was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give_α-product 9a' (55 mg, 71 % yield) and β-product 9b' (22 mg, 29% yield). 9a': [α] D 20 70.5° (c 2.0, CHCI 3 ); FT-IR (film) 3433, 3348, 2943, 2867, 2109, 1730 , 1504, 1453, 1381 , 1336 cm '; Η NMR (300 MHz, CDC I3 ) 6 7.74 (d, J = 7.5Hz, 2H), 7.57 (d, J = 7.5Hz, 2H), 7.25-7.40 (m, 9H), 5.73 (d, J = 8.4Hz, 1 H), 5.24 (d - 12.1 Hz, 1 H), 5.1 7 (d, J - 12.1 , 1 H), 4.73 (d, J = 3.2Hz, 1 H), 4.60 (m, 1 H), 4.41 (dd, J = 10.2, 7.2Hz, 1 H), 4.20-4.31 (m, 4H), 3.82-3.98 (m, 5H), 3.23 (dd, J = 8.0, 3.2Hz, 1 H), 1 .47 (s, 3H), 1.31 (s, 3H), 1.02 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 169.65, 155.88, 143.81 , 143.73, 141.27, 135.04, 128.63, 128.54, 127.71 , 127.60, 125.18, 125.1 1 , 109.67, 98.71 , 77.23, 72.88, 72.39, 68.95, 68.79, 67.73, 67.36, 62.28, 61.10, 54.39, 47.08, 28.26, 26.10, 17.91 , 1 1.90; HRMS (FAB) calc. for C 43 H 56 N 4 0 9 SiK [M + K + ] 839.3453, found 839.3466, 839.3453 ; 9b': [α] D 20 20.6° (c 1.05, CHCI 3 ); FT-IR (film) 3433, 2943, 2866, 21 14, 1729, 1515, 1453, 1382 cm 1 ; 'H NMR (300 MHz, CDC I3 ) 6 7.78 (d, J = 7.4Hz, 2H), 7.63 (t, J = 7.4Hz, 2H), 7.30- 7.44 (m, 9H), 5.91 (d, J = 8.4Hz, 1 H), 5.30 (d, J - 12.4Hz, 1 H), 5.26 (d, J = 12.4Hz, 1 H), 4.65 (m, 1 H), 4.48 (dd, J = 10.0, 2.6Hz, 1 H), 4.39 (t, J = 7.4Hz, 2H), 4.23-4.28 (m, 3H), 3.89-4.04 (m, 3H), 3.85 (dd, J = 10.0, 3.1 Hz, 1 H), 3.78 (m, 1 H), 3.41 (t, J = 8.2Hz, 1 H), 1.58 (s, 3H), 1.36 (s, 3H), 1.08 (m, 21 H); , 3 C NMR (75 MHz, CDCI 3 ) 6 169.37, 155.92, 143.90, 143.69, 141.25, 135.27, 128.55, 128.27, 127.94, 127.68, 127.07, 125.27, 125.21 , 1 i g.g4, 1 10.37, 102.30, 76.87, 73.78, 72.19, 69.68, 67.40, 67.33, 65.44, 61.99, 54.20, 47.06, 28.32, 26.10, 17.89, 1 1.88; HRMS (FAB) calc. for C 43 H 56 N 4 0 9 SiK [M + K + ] 839.3453, found 839.3466. EXAMPLE 21 Coupling of β-trichloroacetimidate 5b' with protected serine derivative 7' in THF Promoted by TMSOTf (0.5 eq.): To a suspension of trichloroacetimidate 5b' (14.4 mg, 0.027 mmol), serine derivative 7' (16.7 mg, 0.040 mmol) and 50 mg 4λ molecular sieve in 0.2 ml of anhydrous THF at -78 °C was added a solution of TMSOTf (2.7 μl, 0.013 mmol) in 50 μl of THF. The reaction was stirred at -78 °C for 2 hours and neutralized with Et 3 N. The reaction mixture was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give the a-product 9a' (18.5 mg, 86% yield). EXAMPLE 22 Coupling of α-trichloroacetimidate 5a with protected serine derivative 7' in THF Promoted by TMSOTf (0.5eq.): To a suspension of trichloroacetimidate 5a' (12.3 mg, 0.023 mmol), serine derivative 7' (14.1 mg, 0.034 mmol) and 50 mg 4λ molecular sieve in 0.2 ml of anhydrous THF at -78 °C was added a solution of TMSOTf (2.2 μl, 0.01 1 mmol) in 45 μl of THF. The reaction was stirred at -78 °C for 4 hours and neutralized with Et 3 N. The reaction mixture was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give the α-product 9a' (11.8 mg, 66% yield). EXAMPLE 23 Coupling of β-trichloroacetimidate 5b' with protected threonine derivative 8: Synthesis of 10a' and 10b': To a suspension of β-trichloroacetimidate 5b' (50.6 mg, 0.093 mmol), threonine derivative 8' (44.0 mg, 0.102 mmol) and 200 mg 4λ molecular sieve in a mixture of 2 ml of anhydrous CH 2 CI 2 and 2 ml of anhydrous hexane at -78 °C was added a solution of TMSOTf (1.85 μl, 0.009 mmol) in 35 μl of CH 2 CI 2 . The reaction mixture was stirred at -78 °C for a half hour, then warmed up to room temperature for 4 hours. The reaction was quenched by Et 3 N. The suspension was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give recovered threonine derivative 7' (28.0 mg), the α-product 10a' (22.0 mg, 29% yield) and the β-product 10b' (3.0 mg, 4% yield). 10a': [α] D 20 55.2° (c 0.88, CHCI 3 ); FT-IR (film) 3430, 2941 , 2866, 2109, 1 730, 1510, 1452, 1380 cm '; ' H NMR (300 MHz, CDC I3 ) 6 7.75 (d, J = 7.5Hz, 2H), 7.59 (d, J - 7.5Hz, 2H), 7.26-7.41 (m, 9H), 5.62 (d, J = 9.4Hz, 1 H), 5.22 (d, J = 12.3Hz, 1 H), 5.18 (d, J = 12.3Hz, 1 H), 4.73 (d, J = 3.6Hz, 1 H), 4.36-4.47 (m, 3H), 4.19-4.32 (m, 4H), 4.09 (m, 1 H), 3.91 (dd, J = 9.8, 6.6Hz, 1 H), 3.83 (dd, J - 9.8, 5.5Hz, 1 H), 3.24 (dd -8.1 , 3.6Hz, 1 H), 1.49 (s, 3H), 1.33 (s, 3H), 1.32 (d, J = 6.0Hz, 3H), 1.05 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 170.12, 156.74, 143.94, 143.69, 141.29, 135.00, 128.65, 128.59, 127.70, 127.10, 125.19, 1 19.96, 109.78, 99.09, 77.22, 73.16, 72.53, 69.03, 67.71 , 67.40, 62.54, 61.61 , 58.84, 47.15, 28.32, 26.1 7, 18.76, 17.94, 1 1.92; HRMS (FAB) calc. for C 44 H 58 N 4 0 9 SiK [M + K + ] 853.3608, found 853.3588; 10b': [α] D 20 92.4° (c 0.47, CH 2 CI 2 ); FT-IR (film) 3434, 3351 , 2940, 2865, 21 1 1 , 1728, 1515, 1455 cm 1 ; ' H NMR (300 MHz, CDC I3 ) 6 7.74 (d, J = 7.5Hz, 2H), 7.59 (t, J = 7.5Hz, 2H). 7.25- 7.40 (m, 9H), 5.68 (d, J = g.3Hz, 1 H), 5.20 (d, J = 12.4Hz, 1 H), 5.1 7 (d, J = 12.4Hz, 1 H), 4.58 (m, 1 H), 4.47 (dd, J - 9.3, 3.4Hz, I H), 4.34 (d, J = 7.8Hz, 2H), 4.18-4.29 (m, 3H), 3.96 (t, J =8.9Hz, 1 H), 3.84 (dd, J = 10.0, 5.2Hz, 1 H), 3.81 (dd, J = 8.2, 5.2Hz, 1 H), 3.65 (m, 1 H), 3.34 (t, J - 8.1 Hz, 1 H), 1.55 (s, 3H), 1.32 (s, 3H), 1.30 (d, J = 6.4Hz, 3H), 1.02 (m, 21 H); 13 C NMR (75 MHz, CDCI 3 ) 6 169.89, 156.73, 143.96, 143.73, 141.27, 135.38, 128.61 , 128.27, 127.93, 127.67, 127.08, 125.26, 1 19.93, 1 10.26, 9g.32, 77.gi , 77.82, 74.03, 73.55, 72.01 , 67.42, 67.25, 65.32, 61.66, 58.61 , 47.12, 28.36, 26.08, 1 7.88, 16.52, 1 1.87; HRMS(FAB) calc. for C 44 H 58 N 4 0 9 SiNa [M + Na + ] 837.386g, found 837.3887. EXAMPLE 24 Coupling of α-glycosyl fluoride 6a' with protected threonine derivative 8' in CH 2 CI 2 promoted by (Cp) 2 ZrCI 2 -AgCI0 4 : To a suspension of AgCI0 4 (25.1 mg, 0.121 mmol), (Cp) 2 ZrCI 2 (17.8 mg, 0.06 mmol) and 150 mg 4λ molecular sieve in 1 ml of anhydrous CH 2 CI 2 at -30 °C was added a solution of α-glycosyl fluoride 6a' (16.3 mg, 0.04 mmol) and threonine derivative 8' (19.2 mg, 0.045 mmol) in 4.0 ml of anhydrous CH 2 CI 2 slowly. The reaction was stirred at -30 °C for 6 hours and quenched with sat. NaHC0 3 . The solution was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with sat. NaHC0 3 , brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give the α-product 10a' (24.8 mg, 75% yield) and the β-product 10b' (3.9 mg, 12% yield). EXAMPLE 25 Coupling of β-glycosyl fluoride 6b' with protected threonine derivative 8' in CH 2 CI 2 promoted by (Cp) 2 ZrCI 2 -AgCI0 4 : To a suspension of AgCI0 4 (24.4 mg, 0.1 18 mmol), (Cp) 2 ZrCI 2 (17.2 mg, 0.059 mmol) and 200 mg 4λ molecular sieve in 1 ml of anhydrous CH 2 CI 2 at -30 °C was added a solution of β-glycosyl fluoride 6b' (15.8 mg, 0.03918 mmol) and threojiine derivative 8' (20.3 mg, 0.04702 mmol) in 4.0 ml of anhydrous CH 2 CI 2 slowly. The reaction was stirred at -30 °C for 10 hours and quenched with sat. NaHC0 3 . The solution was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with sat. NaHC0 3 , brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give the α-product 10a' (22.3 mg, 70% yield) and the β-product 10b' (3.9 mg, 12% yield). EXAMPLE 26 Deprotection of the silyl group of 9a': To a solution of the α-product 9a' (15.0 mg, 0.01873 mmol) in 2 ml of THF at 0 °C were added HOAc (56 μl, 0.978 mmol) and 1M TBAF (240 μl, 0.240 mmol). The reaction was run at 0 °C for 1 hour, and then warmed up to room temperature for 3 days. The mixture was diluted with EtOAc, washed with H 2 0, brine, and finally dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give desired product 11 ' (12.4 mg, 100%). 11 ': [α] D 20 78.3° (c 0.67, CH 2 CI 2 ); FT-IR (film) 3432, 3349, 2987, 2938, 2109, 1729, 1517, 1452, 1382 cm " '; 1 H NMR (300 MHz, CDC I3 ) 6 7.75 (d, J = 7.5Hz, 2H), 7.59 (d, J = 7.5Hz, 2H), 7.27-7.41 (m, 9H), 6.01 (d, J = 9.2Hz, 1 H), 5.21 (d, J = 12.4Hz, 1 H), 5.18 (d, J = 12.4Hz, 1 H), 4.74 (d, J = 3.3Hz, 1 H), 4.58 (m, 1 H), 4.41 (d, J = 7.0Hz, 2H), 4.14-4.23 (m, 3H), 4.02 (dd, J = 5.4, 2.4Hz, 1 H), 3.91-3.97 (m, 2H), 3.68-3.85 (m, 2H), 3.27 (dd, J = 8.2, 3.3Hz, 1 H), 1.48 (s, 3H), 1.33 (s, 3H); 13 C NMR (75 MHz, CDCI 3 ) 6 169.71 , 155.85, 143.78, 143.71 , 141.32, 135.03, 128.59, 127.72, 127.08, 125.08, 1 19.99, 1 10.20, 99.12, 77.20, 73.35, 73.1 1 , 70.22, 68.54, 67.76, 67.04, 62.48, 60.73, 54.66, 47.12, 28.10, 26.14; HRMS (FAB) calc. for C 34 H 37 N 4 0 9 [M + H + ] 645.2560, found 645.2549. EXAMPLE 27 Deprotection of the silyl group of 10a': To a solution of the α-product 10a' (16.0 mg, 0.02 mmol) in 3 ml of THF at O °C were added HOAc (67 μl, 1.18 mmol) and 1M TBAF (300 μl, 0.3000 mmol). The reaction was run at 0 °C for 1 hour, and then warmed up to room temperature for 3 days. The mixture was diluted with EtOAc, washed with H 2 0, brine, and finally dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give desired product 12' (12.1 mg, 94%). 12': [α] D 2 ° 731.8° (c θ.62, CH 2 CI 2 ); FT-IR (film) 3430, 2986, 2936, 2109, 1728, 1515, 1451 , 1382 cm "1 ; Η NMR (300 MHz, CDC I3 ) 6 7.75 (d, J = 7.4Hz, 2H), 7.60 (d, J = 7.4Hz, 2H), 7.25-7.41 (m, 9H), 5.67 (d, J = 9.0Hz, 1 H), 5.21 (br.s, 2H), 4.82 (d, J = 3.2Hz, 1 H), 4.40-4.52 (m, 3 H), 4.33-4.38 (m, 2H), 4.19-4.29 (m, 2H), 4.09 (m, 1 H), 3.75-3.92 (m, 2H), 3.30 (dd, J = 8.0, 3.2Hz, 1 H), 2.04 (m, I H), 1.50 (s, 3H), 1.35 (s, 3H), 1.30 (d, J = 6.2Hz, 3H); 13 C NMR (75 MHz, CDCI 3 ) δ 170.13, 156.69, 143.91 , 143.69, 141.30, 134.98, 128.61 , 127.72, 127.10, 125.20, 1 19.97, 1 10.25, 98.39, 76.26, 73.49, 68.35, 67.75, 67.36, 62.62, 61.31 , 58.69, 47.16, 28.18, 26.24, 18.54; HRMS (FAB) calc. for C 35 H 39 N 4 O g [M + H + ] 659.2716, found 659.2727. EXAMPLE 28 Preparation of compound 14': To a suspension of trichloroacetimidate 13' (332.0 mg, 0.435 mmol), the acceptor 11 ' (140.2 mg, 0.218 mmol) and 1.0 g 4λ molecular sieve in 4 ml of anhydrous CH 2 CI 2 at -30 °C was added a solution of BF 3 Et 2 0 (13.8 μl, 0.109 mmol) in 120 μl of anhydrous CH 2 CI 2 slowly. The reaction mixture was stirred at -30 °C for overnight, then warmed up to room temperature for 3 hours. The reaction was quenched with Et 3 N, filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give crude recovered acceptor 11' which was further converted to compound 9a' (87.0 mg, 0.109 mmol) and crude coupling product which was further reduced to compound 14' by pyridine and thiolacetic acid. The crude coupling product was dissolved in 1 ml of anhydrous pyridine and 1 mi of thiolacetic acid at 0 °C. The reaction mixture was stirred at room temperature for overnight. The solvent was evaporated in vacuo at room temperature and the residue was separated by chromatography on silica gel to give compound 14' (99.6 mg, 72% yield based on 50% conversion of acceptor 11 '). 14': [α] D 20 267.9° (c 4.0 , CHCI 3 ); FT-IR (film) 3361 , 3018, 1751 , 1672, 1543, 1452, 1372 cm "1 ; Η NMR (300 MHz, CDC I3 ) 6 7.72 (d, J = 7.5Hz, 2H), 7.58 (m, 2H), 7.26-7.38 (m, 9H), 6.26 (d, J = 8.2Hz, 1 H), 5.83 (d, J = 9.3Hz, 1 H), 5.59 (d, J = 9.2Hz, 1 H), 5.32 (d, J = 2.7Hz, 1 H), 5.16 (s, 2H), 5.02-5.1 1 (m, 2H), 4.94 (dd, J = 10.4, 3.4Hz, 1 H), 4.59 (d, J = 3.4Hz, 1 H), 4.35-4.52 (m, 6H), 3.60-4.19 (m, 16H), 2.1 1 (s, 3H), 2.05 (s, 3H), 2.02 (s, 3H), 2.01 (s, 3H), 2.00 (s, 3H), 1.93 (s, 3H), 1.91 (s, 3H), 1.83 (s, 3H), 1.48(s, 3H), 1.24 (s, 3H) 13 C NMR (75 MHz, CDCI 3 ) 6 170.33, 170.23, 170.15, 170.07, 169.94, 169.85, 169.19, 155.92, 143.75, 143.64, 141.22, 135.12, 128.62, 128.39, 127.67, 127.01 , 124.99, 1 19.93, 109.81 , 101.12, 100.84, 98.14, 77.21 , 75.49, 74.28, 72.61 , 72.12, 70.74, 69.10, 68.80, 67.61 , 67.38, 67.28, 67.09, 66.64, 62.28. 60.77, 54.25, 53.03, 50.09, 47.09, 27.76, 26.40, 23.18, 23.03, 20.71 , 20.47, 20.36; HRMS(FAB) calc. for C 62 H 75 N 3 0 26 Na [M + Na + ] 1300.4539, found 1300.4520 . EXAMPLE 29 Preparation of compound 15': To a suspension of trichloroacetimidate 13' (305.0 mg, 0.3996 mmol), the acceptor 12' (131.6 mg, 0.1998 mmol) and 1.0 g 4A molecular sieve in 4 ml of anhydrous CH 2 CI 2 at -30 °C was added a solution of BF 3 Et 2 0 (12.7 μl, 0.10 mmol) in 1 15 μl of anhydrous CH 2 CI 2 slowly. The reaction mixture was stirred at -30 °C for overnight, then warmed up to room temperature for 3 hours. The reaction was quenched with Et 3 N, filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give crude recovered acceptor 12' which was further converted to compound 10a' (85.0 mg, 0.104 mmol) and crude coupling product which was further reduced to compound 15' by pyridine and thiolacetic acid. The crude coupling product was dissolved in 1 ml of anhydrous pyridine and 1 ml of thiolacetic acid at 0 °C. The reaction mixture was stirred at room temperature for overnight. The solvent was evaporated in vacuo at room temperature and the residue was separated by chromatography on silica gel to give compound 15' (71.1 mg, 58% yield based on 48% conversion of acceptor 12'). 15': [α] D 20 346.8° (c 0.53, CHCI 3 ); FT-IR (film) 3366, 2986, 1 750, 1673, 1541 , 1452, 1372 cm "1 ; 'H NMR (300 MHz, CDC 13 ) 6 7.73 (d, J = 7.4Hz, 1 H), 7.57 (d, J = 7.4Hz, 2H), 7.27-7.45 (m, 9H), 5.83 (d, J = 9.4Hz, 1 H), 5.74 (d, J = 9.4Hz, 1 H), 5.61 (d, J = 8.9Hz, 1 H), 5.31 (d, J = 3.0Hz, 1 H), 4.91-5.16 (m, 5H), 4.62 (d, J = 3.2Hz, 1 H), 4.32-4.46 (m, 6H), 3.95-4.22 (m, 1 1 H), 3.64-3.84 (m, 3H), 3.57 (m, 1 H), 2.12 (s, 6H), 2.10 (s, 3H), 2.06 (s, 3H), 2.01 (s, 6H), 1.93 (s, 3H), 1.86 (s, 3H), 1.51 (s, 3H), 1.26 (s, 3H), 1.22 (d, J = 5.5Hz, 3H); 13 C NMR (75 MHz, CDCI 3 ) 6 170.70, 170.38, 170.19, 169.g4, 169.86, 169.74, 169.20, 156.34, 143.72, 143.59, 141.26, 134.59, 128.74, 128.37, 127.71 , 127.03, 124.92, 1 19.94, 109.76, 101.48, 100.86, 99.48, 77.20, 76.23, 75.49, 74.41 , 72.74, 72.43, 70.76, 69.26, 69.13, 67.56, 67.45, 67.13, 66.65, 62.29, 60.78, 58.47, 52.83, 50.35, 47.16, 27.86, 26.54, 23.22, 23.03, 20.72, 20.49, 20.37, 18.20; HRMS (FAB) calc. for C 63 H 78 N 3 0 26 [M + H + ] 1292.4871 , found 1292.4890. EXAMPLE 30 Synthesis of compound 1 ': The trisaccharide 14' (105.8 mg, 0.083 mmol) was dissolved in 5 ml of 80% aq. HOAc at room temperature. The reaction mixture was stirred at room temperature for overnight, then at 40 °C for 3 hours. The solution was extracted with EtOAc, washed with sat. NaHC0 3 , H 2 0, brine, and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give diol (93.0 mg, 91 % yield). To a solution of this diol (91.5 mg, 0.074 mmol) in 10 ml of anhydrous CH 2 CI 2 at 0 °C were added catalytic DMAP (4.5 mg, 0.037 mmol), Et 3 N (103 μl, 0.74 mmol) and Ac 2 0 (28 μl, 0.30 mmol) subsequently. The reaction was run for overnight at room temperature. The reaction mixture was diluted with EtOAc, washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give peracetylated compound (88.8 mg, 91 %yield). To a suspension of 10% Pd/C (5.0 mg) in a mixture of 1 ml of MeOH and 0.1 ml of H 2 0 was added a solution of the peracetylated compound (38.5 mg, 0.03 mmol) in 4.0 ml of MeOH. The reaction was stirred under H 2 atmosphere at room temperature for 4 hours. The reaction mixture was passed through a short column of silica gel to remove the catalyst and washed with MeOH. After removal of the solvent, the residue was dissolved in 1.5 ml of DMF and to this solution was added 0.5 ml of morpholine at 0 "C slowly. The reaction was stirred at room temperature for overnight. The solvent was evaporated in vacuo and the residue was separated by chromatography on silica gel to give 29.0 mg material which was further deacetylated in basic condition. The material got previously was dissolved in 50 ml of anhydrous THF and 5 ml of anhydrous MeOH. The solution was cooled to 0 °C and to this solution was added a solution of NaOMe (14.0 mg, 0.26 mmol) in 5 ml of anhydrous MeOH. The reaction was stirred at room temperature for overnight and quenched with 50% aq. HOAc. After evaporation of the solvent, the residue was separated by chromatography on reverse-phase silica gel to give crude product, which was further purified by gel permeation filtration on Sephadex LH-20 to give the final product 1 " (15.1 mg, 77%yield). 1 ': [α] D 20 715.6° (c 0.1 , H 2 0); Η NMR (300MHz, CD 3 OD-D 2 0) 6 4.85 (d, J - 3.4Hz, 1 H), 4.55 (d, J = 7.4Hz, 1 H), 4.46 (d, J - 7.0Hz, 1 H), 4.26 (dd, J = 10.9, 3.5Hz, 1 H), 3.34-4.09 (m, 20H), 2.07 (s, 3H), 2.06 (s, 3H); 13 C NMR (75 MHz, CD 3 0D-D 2 0) 6 175.64, 175.36, 104.61 , 102.98, 99.57, 80.35, 76.94, 76.36, 74.32, 73.88, 72.57, 71.30, 70.82, 70.16, 69.21 , 62.50, 61.62, 56.64, 51.58, 51.22, 23.63, 23.40; HRMS(FAB) calc. for C 25 H 44 N 3 0 18 [M + H + ] 674.2620, found 674.2625. EXAMPLE 31 Synthesis of compound 2': The trisaccharide 15' (70.2 mg, 0.054 mmol) was dissolved in 5 ml of 80% aq. HOAc at room temperature. The reaction mixture was stirred at room temperature for overnight, then at 40 °C for 3 hours. The solution was extracted with EtOAc, washed with sat. NaHC0 3 , H 2 0, brine, and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give diol (67.1 mg, 99% yield). To a solution of diol (65.1 mg, 0.052 mmol) in 8 ml of anhydrous CH 2 CI 2 at 0 °C were added catalytic DMAP (3.2 mg, 0.026 mmol), Et 3 N (72 μl, 0.52 mmol) and Ac 2 0 (20 μl, 0.21 mmol) subsequently. The reaction was run for overnight at room temperature. The reaction mixture was diluted with EtOAc, washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give peracetylated compound (66.0 mg, 95%yield). To a suspension of 10% Pd/C (5.0 mg) in a mixture of 1 ml of MeOH and 0.1 ml of H 2 0 was added a solution of the peracetylated compound (22.1 mg, 0.017 mmol) in 4.0 ml of MeOH. The reaction was stirred under H 2 atmosphere at room temperature for 4 hours. The reaction mixture was passed through a short column of silica gel to remove the catalyst and washed with MeOH. After removal of the solvent, the residue was dissolved in 1.5 ml of DMF and to this solution was added 0.5 ml of morpholine at 0 °C slowly. The reaction was stirred at room temperature for overnight. The solvent was evaporated in vacuo and the residue was separated by chromatography on silica gel to give 29.0 mg material which was further deacetylated in basic condition. The material got previously was dissolved in 50 ml of anhydrous THF and 5 ml of anhydrous MeOH. The solution was cooled to 0 °C and to this solution was added a solution of NaOMe (14.9 mg, 0.276 mmol) in 5 ml of anhydrous MeOH. The reaction was stirred at room temperature for overnight and quenched with 50% aq. HOAc. After evaporation of the solvent, the residue was separated by chromatography on reverse-phase silica gel to give crude product, which was further purified by gel permeation filtration on Sephadex LH-20 to give the final product 2' (8.4 mg, 74%yield). 2': [α] D 20 418.4° (c 0.1 , H 2 0); Η NMR (300MHz, CD 3 0 D -D 2 0) 6 4.91 (d, J = 3.3Hz, 1 H), 4.56 (d, J = 8.2Hz, 1 H), 4.46 (d, J = 7.4Hz, 1 H), 3.52-4.22 (m, 20H), 2.10 (s, 3H), 2.06 (s, 3H), 1.36 (d, J = 6.5Hz, 3H); 13 C NMR (75 MHz, CD 3 OD-D 2 0) 6 175.90, 175.48, 104.20, 103.97, 102.47, 79.75, 78.71 , 76.72, 76.56, 73.92, 73.76, 70.94, 70.52, 70.10, 69.79, 68.98, 62.25, 61.28, 56.25, 51.20, 50.79, 23.51 , 19.44; HRMS(FAB) calc. for C 26 H 46 N 3 0 I6 [M + H + ] 688.2776, found 688.2774. EXAMPLE 32 Preparation of thioglycoside 17': To a suspension of perbenzylated lactal 16' (420 mg, 0.49 mmol) and 600 mg of 4A molecular sieve in 5 ml of anhydrous CH 2 CI 2 was added benzenesulfonamide (1 16 mg, 0.74 mmol) at room temperature. After 10 minutes, the suspension was cooled to 0 °C and l(sym-collidine) 2 CI0 4 was added in one portion. Fifteen minutes later, the solution was filtered through a pad of celite and washed with EtOAc. The organic solution was washed with Na 2 S 2 0 3 , brine and dried over Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give 500 mg of iodosulfonamidate derivative (90%yield). To a solution of ethanethiol (150 μl, 1.98 mmol) in 4 ml of anhydrous DMF at -40 °C was added a solution of LiHMDS (0.88 ml, 0.88 mmol). After 15 minutes, a solution of iodosulfonamidate (450 mg, 0.397 mmol) in 6 ml of anhydrous DMF was added slowly at that temperature. The reaction mixture was stirred at -40 °C for 4 hours, and quenched with H 2 0. The aqueous solution was extracted by EtOAc three times and the combined organic layer was washed with H 2 0, brine and dried over Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give the desired thioglycoside 17' (350 mg, 83%yield) and recover the iodosulfonamidate (60 mg). 17': IR (film) 3020, 3000, 2860, 1480, 1450 cm "1 ; ' H NMR (300 MHz, CDCI 3 ) 6 7.87 (d, J = 7.7 Hz, 2H), 7.1 7-7.45 (m, 33H), 5.01 (d, J = 8.9 Hz, 1 H), 4.93 (d, J = 1 1.4 Hz, 1 H), 4.79 (s, 2H), 4.69 (m, 3H), 4.56 (d, J = 1 1.3 Hz, 2H), 4.30-4.50 (m, 6H), 3.95 (t, J = 5.0 Hz, 1 H), 3.90 (d, J = 2.7 Hz, 1 H), 3.75 (m, 3H), 3.65 (m, 2H), 3.52 (m, 2H), 3.39-3.46 (m, 3H), 2.50 (q, J = 7.4 Hz, 2H), 1.12 (t, J = 7.4 Hz, 3H); HRMS(FAB) calc. for C 62 H 67 O NS 2 K [M + K + ] 1 104.3789, found 1 104.3760. EXAMPLE 33 Preparation of trisaccharide 20': In a round-bottom flask were placed thioglycoside 17'(2.10 g, 1.97 mmol), acceptor 18' (964 mg, 2.95 mmol), di-t-butylpyridine (2.65 ml, 1 1.81 mmol) and 7.0 g of 4A molecular sieve. The mixture was dissolved in 10 ml of anhydrous CH 2 CI 2 and 20 ml of anhydrous Et 2 0. This solution was cooled to 0 °C and then MeOTf (1.1 1 ml, 8.85 mmol) was added to it slowly. The reaction mixture was stirred at 0 °C for overnight. After filtration through a pad of celite, the organic layer was submitted to aqueous work-up. The EtOAc extraction was dried over Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give 20α' (206 mg, 8%) and 20β' (2.26 g, 86%). 20β': IR (film) 3020, 3000, 2860, 1480, 1450 cm 1 ; 'H NMR (300MHz, CDCI 3 ) 6 7.82 (d, J = 7.7 Hz, 2H), 7.20-7.45 (m, 43H), 6.32 (d, J = 6.2 Hz, 1 H), 4.96 (d, J = 9.2 Hz, 1 H), 4.90 (d, J = 6.2 Hz, 1 H), 4.80 (m, 4H), 4.72 (s, 2H), 4.54-4.68 (m, 6H), 4.28-4.48 (m, 6H), 4.07 (br.s, 1 H), 4.00 (t, J = 5.0 Hz, 1 H), 3.90 (s, 1 H), 3.74 (m, 4H), 3.35-3.61 (m, 10H); HRMS(FAB) calc. for C 80 H 83 O 15 NSK [M + K + ] 1368.5123, found 1368.5160. EXAMPLE 34 Preparation of trisaccharide 21 ': In a round-bottom flask were placed thioglycoside 17' (966 mg, 0.906 mmol), acceptor 19' (219 mg, 1.18 mmol), di-t-butylpyridine (1.22 ml, 5.44 mmol) and 2.5 g of 4A molecular sieve. The mixture was dissolved in 5 ml of anhydrous CH 2 CI 2 and 10 ml of anhydrous Et 2 0. This solution was cooled to 0 °C and then MeOTf (0.51 ml, 4.53 mmol) was added to it slowly. The reaction mixture was stirred at 0 °C for 5 hours. After filtration through a pad of celite, the organic layer was submitted to aqueous work-up. The EtOAc extraction was dried over Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give 21 α' (59 mg, 6%) and 21 β' (910 mg, 84%). 21α': IR (film) 3020, 3000, 2860, 1480, 1450 cm '; Η NMR (300MHz, CDCI 3 ) 6 (7.83 (d, J = 7.5 Hz, 2H), 7.12-7.46 (m, 33H), 6.36 (d, J = 6.2 Hz, 1 H), 5.1 1 (d, J = 8.9 Hz, 1 H), 4.98 (d, J = 10.9 Hz, 1 H), 4.93 (d, J = 1 1.6, 1 H), 4.83 (d, J = 8.1 Hz, 1 H), 4.80 (d, J = 1 1.6 Hz, 1 H), 4.68-4.73 (m, 4H), 4.50-4.58 (m, 3H), 4.27-4.32 (m, 4H), 4.27 (d, J = 6.2 Hz, 1 H), 4.05 (m, I H), 3.97 (m, 2H), 3.83 (m, 2H), 3.70 (m, 2H), 3.58 (m, 2H), 3.24-3.49 (m, 4H), 1.52 (s, 3H), 1.41 (s, 3H); HRMS(FAB) calc. for C 69 H 75 0, 5 NSNa [M + Na + ] 1212.4756, found 1212.4720. 21β': IR (film) 3020, 3000, 2860, 1480, 1450 cm " '; Η NMR (300MHz, CDCI 3 ) 6 7.87 (d, J = 7.2 Hz, 2H), 7.19-7.45 (m, 33H), 6.35 (d, J = 6.2 Hz, I H), 4.98 (d, J = 8.9 Hz, 1 H), 4.95 (d, J = 1 1 .6 Hz, 1 H), 4.78 (m, 4H), 4.67 (m, 3H), 4.56 (m, 2H), 4.50 (d, J = 12.0 Hz, 1 H), 4.43 (d, J = 6.2 Hz, 1 H), 4.27-4.39 (m, 4H), 4.04 (d, J = 6.2 Hz, 1 H), 3.97 (t, J = 7.2 Hz, 1 H), 3.90 (d, J = 2.5 Hz, 1 H), 3.73-3.82 (m, 3H), 3.48-3.66 (m, 6H), 3.35-3.42 (m, 3H), 1.43 (s, 3H), 1.30 (s, 3H); HRMS(FAB) calc. for C 69 H 75 0 15 NSNa [M + Na + ] 1212.4755, found 1212.4780. EXAMPLE 35 Preparation of trisaccharide 22': In a flame-dried flask was condensed 30 ml of anhydrous NH 3 at -78 °C. To this liquid NH 3 was added sodium metal (320 mg, 13.95 mmol) in one portion. After 15 minutes, the dry ice-ethanol bath was removed and the dark blue solution was refluxed for 20 minutes. It was cooled down to -78 °C again and a solution of trisaccharide 20' (619 mg, 0.47 mmol) in 6 ml of anhydrous THF was added slowly. The reaction mixture was refluxed at -30 °C for half hour and quenched with 10 ml of MeOH. After evaporation of NH 3 , the basic solution was neutralized by Dowex resin. The organic solution was filtered and evaporated to give crude product which was submitted to acetylation. The crude product was dissolved in 3.0 ml of pyridine and 2.0 ml of Ac 2 0 in the presence of 10 mg of DMAP at 0 °C. The reaction mixture was stirred from 0 °C to room temperature for overnight. After aqueous work-up, the organic layer was dried over Na 2 S0 4 . The solvent was evaporated and the residue was separated by chromatography on silica gel to give peracetylated trisaccharide 22' (233 mg, 59%). 22': [α] D 20 -19.77° (c 1.04, CHCI 3 ); IR(film) 1740, 1360 cm ', 'H NMR (300MHz, CDCI 3 ) 6 6.46 (dd, J = 6.2, 1.5 Hz, 1 H), 5.64 (d, J = 9.1 Hz, 1 H), 5.54 (d, J = 2.0Hz, 1 H), 5.40 (d, J = 4.5 Hz, 1 H), 5.36 (d, J = 2.9 Hz, 1 H), 5.12 (m, 2H), 4.98 (dd, J = 10.4, 3.4 Hz, 1 H), 4.70 (d, J = 6.2 Hz, 1 H), 4.58 (d, J = 7.3 Hz, 1 H), 4.50 (m, 2H), 4.26 (t, J = 5.0 Hz, 1 H), 4.12 (m, 3H), 3.89 (m, 2H), 3.78 (m, 2H), 3.64 (m, 1 H), 2.16 (s, 3H), 2.13 (s, 3H), 2.12 (s, 3H), 2.09 (s, 3H), 2.07 (s, 3H), 2.06 (s, 3H), 2.05 (s, 3H), 2.02 (s, 3H), 1.98 (s, 3H); ,3 C NMR (75 MHz, CDCI 3 ) 6 170.29, 170.14, 169.24, 145.34, 128.20, 100.85, 100.72, 88.86, 75.58, 74.26, 72.58, 72.06, 70.71 , 70.61 , 68.98, 66.77, 66.55, 64.19, 63.53, 62.09, 60.70, 52.97, 23.05, 20.72, 20.56; HRMS(FAB) calc. for C 36 H 49 0 22 NNa [M + Na + ] 870.2645, found 870.2644. EXAMPLE 36 Preparation of trisaccharide donor 23': To a solution of trisaccharide glycal 20' (460 mg, 0.346 mmol) in 3 ml of anhydrous CH 3 CN at -25 °C were added NaN 3 (34 mg, 0.519 mmol) and CAN (569 mg, 1 .4 mmol) subsequently. The mixture was stirred at -25 °C for 8 hours. After aqueous work-up, the organic layer was dried over Na 2 S0 4 . The solvent was evaporated and the residue was separated by chromatography on silica gel to give a mixture of azidonitrate derivatives (134 mg, 27%). This azidonitrate mixture was hydrolyzed in the reductive condition. The azidonitrates was dissolved in 2 ml of anhydrous CH 3 CN at room temperature. EtN(/-Pr) 2 (16 μl, 0.091 mmol) and PhSH (28 μl, 0.272 mmol) were added subsequently. After 15 minutes, the reaction was complete and the solvent was evaporated at room temperature. The hemiacetal derivative (103 mg, 74%) was obtained after chromatography on silica gel. This hemiacetal (95 mg, 0.068 mmol) was dissolved in 2 ml of anhydrous CH 2 CI 2 . To this solution were added 1 ml of CCI 3 CN and 0.5 g of K 2 C0 3 at room temperature. The reaction was run for overnight. After filtration through a pad of celite, the organic solvent was evaporated and the residue was separated by chromatography on silica gel to give 23α' (18 mg, 17%) and 23β' (70 mg, 67%). 23α': 'H NMR (300MHz, CDCI 3 ) 6 8.71 (s, 1 H), 7.96 (d, J = 8.2 Hz, 2H), 6.92-7.50 (m, 33H), 6.56 (d, J = 2.8 Hz, 1 H), 5.02 (m, 3H), 4.92 (d, J = 1 1.6 Hz, 2H), 4.86 (d, J = 1 1.6 Hz, 1 H), 4.22-4.64 (m, 18H), 3.95-4.07 (m, 3H), 3.85 (m, 2H), 3.72 (m, 2H), 3.63 (m, 1 H), 3.35-3.56 (m, 4H), 3.34 (dd, J = 10.3, 2.8 Hz, 1 H). 23β': ' H NMR (300MHz, CDCI 3 ) δ 8.40 (s, 1 H), 8.10 (d, J = 8.1 Hz, 2H), 6.90-7.45 (m, 33H), 6.37 (d, J = 9.4Hz, 1 H), 5.93 (d, J - 8.2 Hz, 1 H), 5.04 (d, J = 1 1.6 Hz, 2H), 4.98 (d, J = 1 1.6 Hz, 1 H), 4.90 (d, J = 1 1.7 Hz, 1 H), 4.83 (d, J = 1 1.7 Hz, 1 H), 4.79 (d, J = 1 1.6 Hz, 1 H), 4.77 (d, j = 1 1.6 Hz, 1 H), 4.72 (d, J =8.2 Hz, 1 H), 4.40-4.63 (m, 8H), 4.19-4.38 (m, 5H), 3.86-4.10 (m, 6H), 3.63 (m, 2H), 3.42-3.50 (m, 4H), 3.35 (m, 2H), 3.25 (d, J - 9.1 Hz, 1 H). EXAMPLE 37 Preparation of trisaccharide donor 24': To a solution of trisaccharide glycal 21 ' (225 mg, 0.264 mmol) in 2 ml of anhydrous CH 3 CN at -15 °C were added NaN 3 (26 mg, 0.40 mmol) and CAN (436 mg, 0.794 mmol) subsequently. The mixture was stirred at -15 °C for overnight. After aqueous work-up, the organic layer was dried over Na 2 S0 4 . The solvent was evaporated and the residue was separated by chromatography on silica gel to give a mixture of azidonitrate derivatives (130 mg, 51 %). This azidonitrate mixture was hydrolyzed in the reductive condition. The azidonitrates (125 mg, 0.129 mmol) was dissolved in 5 ml of anhydrous CH 3 CN at room temperature. EtN(/ ' -Pr) 2 (25 μl, 0.147 mmol) and PhSH (45 μl, 0.441 mmol) were added subsequently. After 15 minutes, the reaction was complete and the solvent was evaporated at room temperature. The hemiacetal derivative (92 mg, 77%) was obtained after chromatography on silica gel. This hemiacetal (80 mg, 0.087 mmol) was dissolved in 5 ml of anhydrous CH 2 CI 2 . To this solution were added 0.9 ml of CCI 3 CN and 0.12 g of K 2 C0 3 at room temperature. The reaction was run for overnight. After filtration through a pad of celite, the organic solvent was evaporated and the residue was separated by chromatography on silica gel to give a mixture of α and β isomer of 24' (71 mg, 77%, α:β 3:1 ). 24': ' H NMR (300MHz, CDCI 3 ) 6 9.55 (s, 1 H, NH of β isomer), 8.71 (s, 1 H, NH of α isomer), 6.54 (d, J = 3.6 Hz, amomeric H of α isomer) EXAMPLE 38 Preparation of trisaccharide donor 25': The azidonitrate derivatives (100 mg, 0.103 mmol) from peracetylated trisaccharide 21 ' was dissolved in 0.5 ml of anhydrous CH 3 CN at room temperature. To this solution was added anhydrous LiBr (45 mg, 0.52 mmol). The mixture was stirred for 3 hours. After aqueous work-up, the solvent was evaporated and the residue was separated by chromatography on silica gel to give compound 25' (91 mg, 90%). 25': ' H NMR (300MHz, CDCI 3 ) 6 6.04 (d, J = 3.6 Hz, 1 H, anomeric H). EXAMPLE 39 Preparation of trisaccharide donor 26': The trisaccharide donor 25' (91 mg, 0.093 mmol) was dissolved in 2 ml of anhydrous THF at 0 °C. To this solution was added LiSPh (100 ml, 0.103mmol). The reaction was run at 0 °C for half hour. The solvent was removed and the residue was separated by chromatography on silica gel to give compound 26' (61 mg, 66%). 26": IR (film) 3000, 2100, 1750, 1680, 1500 cm '; 'H NMR (300MHz, CDCI 3 ) 6 7.61 (m, 2H), 7.39 (m, 3H), 5.50 (d, J = 9.1 Hz, 1 H), 5.35 (m, 2H), 5.1 1 (m, 2H), 4.96 (dt, J = 10.5, 3.5 Hz, 1 H), 4.84 (dd, J = 10.2, 3.0 Hz, 1 H), 4.50 (m, 4H), 4.16 (m, 3H), 3.59-3.90 (m, 8H), 2.15 (s, 3H), 2.10 (s, 3H), 2.08 (s, 3H), 2.06 (s, 6H), 2.05 (s, 3H), 2.04 (s, 3H), 1.97 (s, 3H), 1.87 (s, 3H). EXAMPLE 40 Preparation of trisaccharide donor 27': The trisaccharide 21' (860 mg, 0.722 mmol) was dissolved in 2 ml of pyridine and 1 ml of Ac 2 0 in the presence of 10 mg of DMAP. The reaction was run at 0 °C to room temperature for overnight. After aqueous work-up, the solvent was removed and the residue was dissolved in 10 ml of MeOH and 5 ml of EtOAc at room temperature. To this solution were added Na 2 HP0 4 (410 mg, 2.89 mmol) and 20% Na- Hg (1.0 g, 4.35 mmol). The reaction was run for 2 hours and aqueous work-up followed. After removal of the organic solvent, the residue was separated by chromatography on silica gel to give N-acetyl trisaccharide glycal (740 mg, 94%). The trisaccharide glycal (624 mg, 0.571 mmol) was dissolved in 3 ml of anhydrous CH 3 CN at -40 °C. To the solution were added NaN 3 (56 mg, 0.86 mmol) and CAN (93g mg, 1.71 mmol) subsequently. The mixture was stirred at -40 °C for 4 hours. After aqueous work-up, the organic solvent was removed and the residue was separated by chromatography on silica gel to give a mixture of α and β azidonitrate anomers (191 mg, 27%). This mixture of anomers (1 72 mg, 0.137 mmol) was dissolved in 1 ml of CH 3 CN at room temperature. To the solution were added EtN( -Pr) 2 (24 μl, 0.137 mmol) and PhSH (42 μl, 0.410 mmol) subsequently. The reaction was complete in half hour and the solvent was blown off. Separation on column afforded desired hemiacetal (170 mg). This hemiacetal was dissolved in 1 ml of CH 2 CI 2 at room temperature. To the solution were added 1 ml of CCI 3 CN and 500 mg of K 2 C0 3 . The reaction was run at room temperature for overnight. After filtration through a pad of celite, the organic solvent was removed and the residue was separated by chromatography on silica gel to give desired α- trichloroacetimidate 27' (70 mg, 42%). 27': IR (film) 3000, 2120, 1670, 1490, 1450 cm '; 'H NMR (300MHz, CDCI 3 ) δ 8.62 (s, 1 H), 7.06-7.48 (m, 30H), 6.44 (d, J = 3.0 Hz, 1 H), 5.21 (d, J = 1 1.4 Hz, 1 H), 5.03 (m, 2H), 4.89 (d, J = 1 1.0 Hz, 1 H), 4.80 (d, J = 1 1.3 Hz, 1 H), 4.69 (d, J = 1 1.1 Hz, 1 H), 4.64 (d, j = 7.8 Hz, 1 H), 4.44-4.58 (m, 5H), 4.18-4.36 (m, 7H), 3.96-4.08 (m, 3H), 3.72-3.81 (m, 3H), 3.38-3.62 (m, 6H), 3.31 (dd, J - 7.0, 2.7 Hz, 1 H), 1.59 (s, 3H), 1.31 (s, 3H), 1.14 (s, 3H); HRMS(FAB) calc. for C 68 H 74 0, 5 N 5 CI 3 Na [M + Na +] 1316.4145, found 1316.41 10. EXAMPLE 41 Coupling of trisaccharide donor 23α' with methyl N-Fmoc Serinate: To a solution of trisaccharide donor 23α' (70 mg, 0.046 mmol), methyl N-Fmoc serinate (23.4 mg, 0.068 mmol) and 300 mg of 4λ molecular sieve in 0.5 ml of THF at -78 °C was added TMSOTf (4.6 μl, 0.023 mmol). The reaction was stirred at -35 °C for overnight. The reaction was quenched by Et 3 N and the solution was filtered through a pad of celite. The filtrate was evaporated and the residue was separated by chromatography on silica gel to give 29α' (70 mg, 90%) and 29β' (7.0 mg, 9.0%). EXAMPLE 42 Coupling of trisaccharide donor 24' with benzyl N-Fmoc serinate: To a solution of trisaccharide donor 24' (33 mg, 0.030 mmol), benzyl N-Fmoc serinate (33.0 mg, 0.075 mmol) and 100 mg of 4λ molecular sieve in 0.3 ml of THF at -78 °C was added TMSOTf (6.0 μl, 0.030 mmol). The reaction was stirred from -78 °C to room temperature for 2 hours. The reaction was quenched by Et 3 N and the solution was filtered through a pad of celite. The filtrate was evaporated and the residue was separated by chromatography on silica gel to give 30' (8.6 mg, 22%, α:β 2:1 ). 30': I R (film) 3400, 3000, 2100, 1 740, 1500 cm " '; 'H NMR (300MHz, CDCI 3 ) 6 6.25 (d, J = 8.4 Hz, 2/3H), 5.90 (d, J = 8.6 Hz, 1/3H), 5.76 (d, J = 9.0 Hz, 1/3H), 5.71 (d, J = 9.0 Hz, 2/3); MS(CI) 1306 [M + ]. EXAMPLE 43 Coupling of trisaccharide donor 25α' with benzyl N-Fmoc serinate: To a solution of benzyl N-Fmoc serinate (45 mg, 0.107 mmol), AgCI0 4 (37.0 mg, 0.179 mmol) and 200 mg of 4A molecular sieve in 0.6 ml of anhydrous CH 2 CI 2 was added a solution of trisaccharide donor 25α' (88 mg, 0.0893 mmol) in 0.5 ml of CH 2 CI 2 slowly. The reaction was run at room temperature for overnight. After filtration through a pad of celite, the solvent was removed and the residue was separated by chromatography on silica gel to give the coupling product 30' (66 mg, 56%, α:β 3.5 :1). EXAMPLE 44 Coupling of trisaccharide donor 26β' with benzyl N-Fmoc serinate: To a solution of benzyl N-Fmoc serinate (45 mg, 0.107 mmol), trisaccharide donor 26β' (23 mg, 0.023 mmol) and 50 mg of 4A molecular sieve in 1.0 ml of anhydrous CH 2 CI 2 at O °C was added a solution of NIS (6.2 mg, 0.027 mmol) and TfOH (0.24 μl, 0.003 mmol) in 0.5 ml of CH 2 CI 2 slowly. The reaction was run at 0 0C for 1 hour. The reaction was quenched by Et 3 N and aqueous work- up followed. The organic solvent was dried over Na 2 S0 4 . After removal of the solvent, the residue was separated by chromatography on silica gel to give the coupling product 30' (12.1 mg, 40%, α:β 2 :1 ). EXAMPLE 45 Coupling of trisaccharide donor 27α' with benzyl N-Fmoc serinate: To a solution of trisaccharide donor 27α' (40.1 mg, 0.029 mmol), benzyl N-Fmoc serinate (18.0 mg, 0.044 mmol) and 200 mg of 4λ molecular sieve in 2.0 ml of THF at -20 °C was added TMSOTf (1.8 μl, 0.009 mmol). The reaction was stirred from -20 °C to room temperature for 3 hours. The reaction was quenched by Et 3 N and aqueous work-up followed. After dried over Na 2 S0 4 , the filtrate was evaporated and the residue was separated by chromatography on silica gel to give 31 ' (24 mg, 51 %). 31 ': IR(film) 3000, 2920, 2860, 2100, 1720, 1665, 1500, 1480, 1450 cm "1 ; 'H NMR (300MHz, CDCI 3 ) 6 7.78 (m, 2H), 7.65 (d, J = 7.5 Hz, 1 H), 7.60 (d, J = 7.5 Hz, 1 H), 7.20-7.42 (m, 39 H), 6.18 (d, J = 7.8 Hz, I H), 6.05 (d, J = 7.3 Hz, 1 H), 5.23 (s, 2H), 4.95-5.02 (m, 3H), 4.80 (s, 2H), 4.78 (d, J = 2.8 Hz, 1 H, anomeric H), 4.72 (s, 2H), 4.58 (m, 4H), 4.37- 4.52 (m, 6H), 4.24-4.31 (m, 2H), 4.20 (m, 1 H), 4.08 (m, 2H), 3.92-4.02 (m, 5H), 3.78-3.85 (m, 5H), 3.65 (m, I H), 3.58 (t, J = 6.2Hz, 1 H), 3.36-3.46 (m, 5H), 3.26 (dd, J = 7.5, 2.8 Hz, 1 H), 1.85 (s, 3H), 1.48 (s, 3H), 1.34 (S, 3H); HRMS(FAB) calc. for C 90 H 95 O 19 N 5 Na [M + Na + ] 1572.6520, found 1572.6550. EXAMPLE 46 Coupling of trisaccharide donor 28' with benzyl N-Fmoc serinate: To a solution of trisaccharide donor 28' (α:β 1 :1 )062 mg, 0.163 mmol), benzyl N-Fmoc serinate (48.0 mg, 0.097 mmol) and 300 mg of 4λ molecular sieve in 2.0 ml of THF at -78 °C was added BF 3 Et 2 0 (0.5 eq., 0.082 mmol) in CH 2 CI 2 . The reaction was stirred from -78 °C to room temperature for 2 hours. The reaction was quenched by Et 3 N and aqueous work-up followed. After dried over Na 2 S0 4 , the filtrate was evaporated and the residue was separated by chromatography on silica gel to give 32' (81 mg, 67%). 32': IR(film) 3420, 3020, 2940, 2880, 2120, 1 745, 1500, 1450 cm "1 , Η NMR (300MHz, CDCI 3 ) 6 7.74 (d, J = 7.4 Hz, 2H), 7.60 (t, J - 7.5 Hz, 2H), 7.20-7.39 (m, 9H), 5.85 (d, J = 8.4 Hz, 1 H), 5.48 (d, J = 12.6 Hz, 1 H), 5.32 (d, J = 3.4 Hz, 1 H), 5.19 (d, J - 12.6 Hz, 1 H), 5.07 (d, J = 8.0 Hz, 1 H), 4.90 (dd, J = 10.3, 3.4 Hz, 1 H), 4,83 (t, J = 10.3 Hz, 1 H), 4.72 (d, J = 9.3 Hz, 1 H), 4.67 (d, J - 9.6 Hz, 1 H), 3.80-4.47 (m, 9H), 3.62 (t, J = 9.5 Hz, 1 H), 3.32-3.42 (m, 2H), 2.93 (d, J - 7.7 Hz, 1 H), 2.14 (s, 3H), 2.08 (s, 6H), 2.04 (s, 3H), 2.02 (s, 3H), 1.95 (s, 3H), 1.55 (s, 3H), 1.34 (s, 3H). EXAMPLE 47 Coupling of trisaccharide donor 28β' with benzyl N-Fmoc serinate: To a solution of trisaccharide donor 28β' (12.0 mg, 0.012 mmol), benzyl N-Fmoc serinate (9.0 mg, 0.022 mmol) and 100 mg of 4λ molecular sieve in 0.5 ml of THF at -40 °C was added BF 3 Εt 2 0 (1.5 eq. , 0.018 mmol) in CH 2 CI 2 . The reaction was stirred from -40 °C to room temperature for 2 hours. The reaction was quenched by Et 3 N and aqueous work-up followed. After dried over Na 2 S0 4 , the filtrate was evaporated and the residue was separated by chromatography on silica gel to give 32' (5.2 mg, 35%). NIS/TfOH 2.3-ST Antigen Precursor A mixture of thioethyl glycosyl donor 30 (52 mg, 0.064 mmol) and 6-TBDMS acceptor 31 (94 mg, 0.13 mmol) were azeotroped with benzene (4 x 50 mL), then placed under high vacuum for 1 h. The mixture was placed under nitrogen, at which time 4A mol sieves (0.5 g), CH 2 CI 2 (5 mL), and NIS (36 mg, 0.16 mmol) were added. The mixture was cooled to 0 °C, and trifluoromethanesulfonic acid (1 % in CH 2 CI 2 , 0.96 mL, 0.064 mmol) was added dropwise over 5 min. The suspension was warmed to ambient temperature immediately following addition and stirred 20 min. The mixture was partitioned between EtOAc (50 mL) and sat. NaHC0 3 (50 mL). The phases were separated, and the organic phase washed with brine (50 mL), dried (Na 2 S0 4 ), and concentrated. The residue was purified by flash chromatography on silica gel (4:1 , EtOAc:hexanes) to provide 59 mg (62%) of the trisaccharide 32 as a colorless crystalline solid. Le y Antigen Precursor To thiodonor 32 (44.0 mg, 29.5 μmol) and acceptor 31 (42.4 mg, 59.0 μmol) (azeotroped 3 times with toluene) were added CH 2 CI 2 and freshly activated 4λ molecular sieves. The mixture was stirred for 20 min, then cooled to 0°C. N-iodosuccinimide (16.6 mg, 73.8 μmol) was added, followed by the dropwise addition of a 1 % solution of TfOH in CH 2 CI 2 . The red mixture was stirred at 0°C for 5 min, then was diluted with EtOAc. The organic phase was washed with sat. NaHC0 3 , sat. Na 2 S 2 0 3 , and brine, dried over MgS0 4 , then concentrated in vacuo. Flash chromatography (1 :1 EtOAc/CH 2 CI 2 to 2:1 EtOAc CH 2 CI 2 ) afforded 43.2 mg (68%) of the coupled product 34. Coupling of 6-Trichloroacetimidate with Protected Threonine To a solution of trichloroacetimidate 35 (98 mg, 0.13 mmol), threonine derivative 36 (70 mg, 0.167 mmol) and 100 mg 4λ molecular sieve in 6 ml of anhydrous CH 2 CI 2 at -30 e C was added TMSOTf (14 mL, 0.07 mmol). The reaction was stirred at -30°C for 1 hour, then neutralized with Et 3 N. The reaction mixture was filtered through a pad of celite and washed with EtOAc. The filtrate was washed with H 2 0, brine and dried over anhydrous Na 2 S0 4 . After evaporation of the solvent, the residue was separated by chromatography on silica gel to give β-product 37β (56 mg, 42%) and the α-product 37α (57 mg, 42%). Discussion The synthetic approach taken in the present invention encompasses four phases (Figure 2). First, the complete glycodomain is assembled in the form of an advanced glycal. This is followed by efficient coupling to a serine, threonine or analogous residue. The third stage involves peptide assembly incorporating the full glycosyl domain amino acids into the peptide backbone. The concluding phase involves global deprotection either in concurrent or segmental modes. The synthetic starting point was the readily available glycal 2 (Figure 3). (Oxidation of this compound with dimethyldioxirane and subsequent coupling of the resultant epoxide with 6-O-TIPS-galactal was promoted by ZnCI 2 in the standard way. Toyokuni, T.; Singhal, A.K.; Chem. Soc. Rev. 1995, 24, 231. Acetylation of the crude product yielded disaccharide 3 in high yield and stereoselectivity. Removal of the TIPS protecting group under mild conditions set the stage for attachment of sialic acid to acceptor 4. The use of sialyl phosphite 5 as the donor, under promotion of catalytic amounts of TMSOTf, consistently provided high yields (80 - 85%) of a 4:1 mixture of products. Martin, T.J., et al., Glycoconjugate j. 1993, 10, 16. Sim, M.M, et al., }. Am. Chem. Soc. 1993, 1 15, 2260. Thus, the advanced glycal 6 ("2,6-ST glycal") is available in four steps with high efficiency. The trisaccharide glycal 6 was submitted to azidonitration as shown (Figure 3). Compound 7 thus obtained in 60% yield lent itself to conversion to a variety of donor constructs (see 8 - 11). For instance, α-bromide 8 can be used as a donor directly or could be converted to β-phenylthioglycoside 11 with lithium thiophenoxide in a stereoselective manner. Alternatively, mixtures of nitrates 7 was hydrolyzed and the resulting hemiacetal converted to 1 :1 mixture of α:β trichloroacetamidates (9) and diethylphoshites (10) in high yields (Figure 3). (Nitrate hydrolysis: Gauffeny, F., et al., Carbohydr. Chem. 1991, 219, 237. Preparation and application of trichloroacetamidates: Schmidt, R.R. and Kinzy, W.; Adv. Carbohydr. Chem. Biochem. 1994, 50, 21. Phosphite donors: Kondo, H., et al.; ]. Org. Chem. 1994, 59, 864.) Table I. Reaction of 11 with N-FMOC-Ser(OH)-OBn. The availability of various donor types (8-11) enabled the investigation of the direct coupling of (2,6)-ST trisaccharide to benzyl ester of N-Fmoc-protected L-serine and L- threonine. The results are summarized in Table 1. As with Fmoc protected L-threonine as the acceptor, all of the donors afforded the α-O glycosyl threonine system in high stereoselectivity. By contrast, the outcome of the coupling reactions with similarly protected L-serine acceptors was dependent on the character of the donor and on the reaction conditions. In all cases, the desired α-anomer 12 was the major product. (For previous attempts to couple a trisaccharide donor to serine, in which β-anomers were isolated as the major products, see: Paulsen, H. et al., Liebigs Ann Chem 1988, 75; jima, H.; Ogawa, T., Carbohydr. Res. 1989, 186, 95.) With donor 10 the ratio of desired α-product: undesired β- glycoside was ca 30:1. The glycopeptide assembly phase was entered with building units 14 and 15, thereby reducing the number of required chemical operations to be performed on the final glycopeptide. Thus, compounds 14 and 15 were obtained in two steps from 12 and 13, respectively. The azide functionality was transformed directly to N-acetyl groups by the action of CH 3 COSH in 78-80% yield and the benzyl ester was removed quantitatively by hydrogenolysis (Figure 4). Paulsen, H., et al., Liebigs Ann Chem. 1994, 381. The glycopeptide backbone was built in the C-N-termmus direction (Figure 4). Iteration of the coupling step between the N-terminus of a peptide and protected glycosyl ammo acid, followed by removal of the FMOC protecting group provided protected pentapeptide 16. The peptide coupling steps of block structures such as 12 and 13 proceeded in excellent yields. Both IIDQ and DICD coupling reagents work well (85-90%). FMOC deprotection was achieved under mild treatment with KF in DMF in the presence of 18- crown-6. Jiang, J., et al., Synth. Commun. 1994, 24, 187. The binal deblocking of glycopeptide 16 was accomplished in three stages: (i) Fmoc removal with KF and protection of the ammo terminus with acetyl group; (n) hydrogenolysis of the benzyl ester; and (in) final saponification of three methyl esters, cyclic carbonates and acetyl protection with aqueous NaOH leading to glycopeptide mucin model 1 (Figure 4). The orthogonal exposure of both N- and C-termini provided an opportunity for further extension of the glycopeptide constructs via fragment joining. In order to demonstrate the viability of such claims, a nonapeptide with ST triad 19 was made by means of coupling tπpeptide 18 to hexapeptide 17 (see Figure 5). The previous deprotection protocol provided nonapeptide mucin model 20, wherein the o-glycosylated serine-threonme triad had been incorporated in the middle of the peptide. Vaccination with Tn Cluster Constructs in Mice The present invention provides anti-tumor vaccines wherein the glycopeptide antigen disclosed herein is attached to the lipopeptide carrier PamCys. The conjugation of the antigen to the new carrier represents a major simplification in comparison to traditional protein carriers. Tables 2 and 3 compare the immunogeniαty of the new constructs with the protein carrier vaccines in mice. These novel constructs proved immunogenic in mice. As shown in the Tables, the Tn-PamCys constructs elicit high titers of both IgM and IgG after the third vaccination of mice. Even higher titers are induced after the fifth vaccination. The Tn- KLH vaccine yields stronger overall response. However, the relative ratio of IgM/lgG differs between the two vaccines. Tn-KLH gives higher IgM/lgG ratio than the Tn Pamcys. In a relative sense, the novel Tn-PamCys vaccine elicits a stronger IgG response. In contrast to protein carrier vaccines, the adjuvant QS-21 does not provide any additional enhancement of immunogenicity. Accordingly, the PamCys lipopeptide carrier may be considered as a "built- in" immunostimulant/adjuvant. Furthermore, it should be noted that QS-21 enhances the IgM response to Tn-PamCys at the expense of IgG titers. A vaccine based on PamCys carriers is targeted against prostate tumors. Table 2. Antibody Titers by Elisa against Tn-Cluster: IQ ttg Tn cluster-Pam Pre-serum 1 Q day? post 3rd Group leM IgG IgM IgQ 1.1 50 0 450 450 1.2 50 0 1350 50 1.3 50 0 4050 150 1.4 0 0 4050 150 1.5 0 0 450 1350 10 ug Tn cluster-pam + QS-21 2.1 50 0 1250 50 2.2 0 0 1350 0 2.3 0 0 1350 50 2.4 0 0 1350 150 2.5 50 0 1350 150 3 uε Tn cluster KLH + QS-21 3.1 0 0 12150 450 3.2 0 0 12150 4050 3.3 0 0 36450 450 3.4 0 0 36450 450 3.5 0 0 36450 1350 3 u Tn cluster BSA + OS-21 4.1 0 0 450 1350 4.2 0 0 150 4050 4.3 0 50 450 450 4.4 0 0 450 150 4.5 0 0 1350 150 0.3 μg/well antigen plated in alcohol; serum drawn 1 1 days post 3rd vaccine. Table 3. Antibody Titers by Elisa against Tn-Cluster: Tn Cluster-Pam Pre-serum (before 5th Vaccination) Post Serum (10 davs after 5th Vaccination) Group IgM leG leM IgG 1.1 2560 200 640 5120 1.2 25.600 800 1280 320 1.3 640 160 640 1280 1.4 2560 1280 25.600 5120 1.5 640 5120 2560 5120 Tn Cluster-Pam + OS-21 2.1 6400 1280 128.000 0 2.2 3200 160 5120 200 2.3 3200 1280 16.000 640 2.4 6400 640 8000 200 2.5 5120 80 64.000 2560 Tn Cluster-K H 3.1 6400 1600 25.600 25.600 3.2 2560 3200 128.000 25.600 3.3 16.000 8000 128.000 25.600 3.4 640 12.800 5120 25.600 3.5 5120 12.800 25.600 3200 Tn-Cluster-BSA 4.1 2560 12.800 2560 * 4.2 800 200 128.000 400 4.3 400 2560 6400 400 4.4 800 2560 12800 2560 4.5 1280 200 3200 3200 0.2 μg/well plated in ethanol. *ND Table 4. Tn-Cluster FACS Analysis; Serum Tested 1 1 Days Post 3rd Vaccination. FACS analysis using LSC cell line (Colon Cancer Cell line). Group IβC (% Gated) leM (% Gated) Tn Cluster Pam 1-1 93.95 16.59 1-2 19.00 66.15 1-3 54.45 40.51 1-4 46.99 39.98 1-5 3.07 32.83 Tn Cluster-Pam + QS-21 2-1 12.00 76.78 2-2 2.48 36.76 2-3 20.27 46.41 2-4 10.64 55.29 2-5 3.37 38.95 Tn-Cluster-KLH 3-1 96.36 66.72 3-2 93.12 45.50 3-3 97.55 32.96 3-4 94.72 49.54 3-5 83.93 64.33 Tn-Cluster-BSA 4-1 80.65 41.43 4-2 90.07 31.68 4-3 42.86 54.03 4-4 95.70 63.76 4-5 92.14 51.89 Table 5. Results of Tn-trimer-Cys-KLH and Tn-trimer-Cys-BSA (MBS cross-linked) Conjugates Amt of Carbohydrate conjugate & KLH used for Final Amt of Carbohydrate 0 //o Conjugation Conjugation Recovered Recovered μg of μg of Carbo. KLH Volume Carbohydrate LH Carbohydrate KLH carbohydrate/100/. I KLH/100I/I " n-trimer- Cys-KLH 2.0 mg 5.0 mg 4.25 ml 141 .1 74 μg 3612.5 μg 7% 72.25% 3.321 85 2.5* 5.65 (3μg/mouse; c 300μl/vial1) CD (0 " n-trimer-Cys-BSA 2.0 2.0 3.25 108.9 2762.5 5.445 100 3.35 85 c 10.89 (3μg/mouse; H m 170μl/vialfl en σs m m H After concentration. ^ Approximate amount. c r m ro σ> A Total Synthesis of the Mucin Related F1α Antigen The present invention provides derived mimics of surfaces of tumor tissues, based mainly on the mucin family of glycoproteins. Ragupathi, G., et al., Angew. Chem. Int. Ed. Engl. 1997, 36, 125. (For a review of this area see Toyokuni, T.; Singhal, A. K. Chem. Soc. Rev. 1995, 24, 231 ; Dwek, R. A. Chem. Rev. 1996, 96, 683.) Due to their high expression on epithelial cell surfaces and the high content of clustered O-lmked carbohydrates, mucins constitute important targets for antitumor immunological studies. Mucins on epithelial tumors often carry aberrant α-O-linked carbohydrates. Finn, O.J., et al., Immunol. Rev. 1995, 145, 61 ; Saitoh, O. et al., Cancer Res. 1991, 51, 2854; Carlstedt, I.; Davies, J. R. Biochem. Soc. Trans. 1997, 25, 214. The identified F1 α antigens 1 ' and 2' represent examples of aberrant carbohydrate epitopes found on mucins associated with gastric adenocarcmomas (Figure 22A). Yamashita, Y., et al., J. Nat. Cancer Inst. 1995, 87, 441 ; Yamashita, Y., et al., Int. j. Cancer 1994, 58, 349. Accordingly, the present invention provides a method of constructing the F1 α epitope through synthesis. A previous synthesis of F1 α is by Qui, D.; Koganty, R. R. Tetrahedron Lett. 1997, 38, 45. Other prior approaches to α-O-lmked glycopeptides include Nakahara, Y., et al., in Synthetic Oligasacchandes, Indispensable Probes for the Life Sciences ACS Symp. Ser. 560, pp 249-266 (19g4); Garg, H. G., et al., Adv. Carb. Chem. Biochem. 1994, 50, 277; Paulsen, H., et al., ). Chem. Soc, Perkm Trans. 1, 1997, 281 ; Liebe, B.; Kunz, H. Angew. Chem. Int. Ed. Engl. 1997, 36, 618; Elofsson, M., et al., Tetrahedron 1997, 53, 369; Memjohanns, E., et al., }. Chem. Soc, Perkm Trans. 1, 1996, 985; Wang, Z.-G., et al., Carbohydr. Res. 1996, 295, 25; Szabo, L., et al., Carbohydr. Res. 1995, 274, 1 1. Tthe F1 α structure could be constructed from the three principal building units I-III (Figure 22A). Such a general plan permits two alternative modes of implementation. (For a comprehensive overview of glycal assembly, see: Bilodeau, M. T.; Danishefsky, S. J. Angew. Chem. Int. Ed. Engl. 1996, 35, 1381. For applications toward the synthesis of carbohydrate tumor antigen based vaccines, see Sames, D., et al., Nature 1997, 389, 587; Park, T. K., et al., }. Am. Chem. Soc. 1996, 1 18, 1 1488; and Deshpande, P. P.; Danishefsky, S. J. Nature 1997, 387, 164.) First, a GalNAc-seπne/threonine construct might be assembled in the initial phase. This would be followed by the extension at the "non-reducing end" (II + III, then I). Alternatively, the entire glycodomam could be assembled first in a form of trisaccharide glycal (I + II). This step would be followed by coupling of the resultant trisaccharide donor to a serine or threonine ammo acid residue (cf. II). Both strategies are disclosed herein. The first synthetic approach commenced with preparation of monosacchaπde donors 5a'/b' and 6a'/b' (Figure 22B). The protecting groups of galactal (cf. II) were carefully chosen to fulfill several requirements. They must be stable to reagents and conditions in the azidonitration protocol (vide infra). Also, the protecting functions must not undermine the coupling step leading to the glycosyl amino acid. After some initial experimentation, galactal 3' became the starting material of choice. The azidonitration protocol (NaN 3 , CAN CH 3 CN, -20 °C) provided a 40% yield of 1 :1 mixture of 4a' and 4b'. Lemieux, R. U.;Ratcliffe, R. M. Can. ). Chem. 1979, 57, 1244. Both anomers were hydrolyzed and then converted to a 1 :5 mixture of trichloroacetimidates 5a' and 5b' in good yield (84%). Schmidt, R. R.; Kinzy, W. Adv. Carbohydr. Chem. Biochem. 1994, 50, 84. Alternatively, hydrolysis of nitrate 4' followed by use of the DAST reagent (Rosenbrook, Jr. W., et al., Tetrahedron Lett. 1985, 26, 3; Posner, G. H.; Haines, S. R. Tetrahedron Lett. 1985, 26, 5) yielded a 1 :1 mixture of fluoride donors 6a' and 6b'. In both cases the α/β anomers were separable, thus allowing the subsequent investigation of their behavior in the coupling event. The best results obtained from the coupling of donors 5'-6' to serine or threonine acceptors bearing the free side chain alcohol, with protected carboxy and amino moieties are summarized in Table 5a. The trichloroacetimidate donor type 5' provided excellent yields in coupling reactions with the serine derived alcohol 7'. After optimization, donor 5b' in the presence of TMSOTf in THF (entry 2, Table 5a) provided 86% yield of pure α-product 9'. Interestingly, the donor 5a' also provided α-glycoside 9' exclusively. The coupling of donor 5b' to threonine, though stereoselective, was low yielding. In this instance the fluoride donors 6a' and 6b', promoted by Cp 2 ZrCI 2 /AgCI0 4 provided desired glycosyl threonine 10' in excellent yield (82-87%) though with somewhat reduced selectivity (6:1 , α:β). Ogawa, T. Carbohydrate Res. 1996, 295, 25. Thus, both sets of donors proved complementary to one another and glycosyl serine 9' as well as glycosyl threonine 10' were in hand in high yield and with excellent margins of stereoselectivity. It was found that the configurations at the anomeric centers of these donors had no practical effect on the stereochemical outcome of their coupling steps. This result differs from the finding with commonly used 2-deoxy-2-azido-tri- O-acetylgalactose-1-O-trichloroacetimidate. See Schmidt, R. R.; Kinzy, W., id. In that case each anomer yields a different ratio of α/β products (see below). Table 5a. R = H (9 ') R = CH 3 (10 ' ) x Catalyst/ promotor α::β (%) α::β (%) -0(CNH)CCI 3 (5b') TMSOTf (0.1eq), CH 2 CIJHex 7:3(100%) 7:1(33%) -0(CNH)CCI 3 (5b') TMSOTf (0.5eq), THF 1:0(86%) 1:0(15%) -0(CNH)CCI 3 (5a') TMSOTf (0.1eq), THF 1:0(66%) -F (6a ' ) Cp 2 ZrCI 2 /AgCI0 4 (2eq), CH 2 CI 2 2: 1 (89%) 6: 1 (87%) -F(6b ' ) Cp 2 ZrCIJAgCI0 4 (2eq), CH 2 CI 2 2: 1 (91 %) 6: 1 (82%) The TIPS group at position 6 was quantitatively removed with TBAF and AcOH to give acceptors 11 ' and 12' (Figure 23). The final coupling to lactosamine donor 13' was performed in the presence of BF 3 OEt 2 in THF. The crude products from this apparently stereoselective coupling step were converted to compounds 14' and 15', respectively with thiolacetic acid. Paulsen, H., et al., Liebigs Ann.Chem. 1994, 381. These glycosyl amino acids represent suitable units for the glycopeptide assembly. In order to confirm their structure, we executed global deprotection. This was accomplished in five steps yielding free F1 α antigen 1 ' and 2' in 70% and 73% yield, respectively (Figure 23). The glycosidic linkages were not compromised under the conditions of the acidic and basic deprotection protocols. A direct coupling Is provided of trisaccharide donors which are synthesized through glycal assembly (Bilodeau, M. T.; Danishefsky, S. J. Angew. Chem. Int. Ed. Engl. 1996, 35, 1381 ) using suitably protected serine or threonine amino acids. This logic was discussed earlier under the formalism I + II followed by coupling with III. The trisaccharide donors 23'-27' were prepared as outlined in Figure 24. Readily available lactal 16' (Kinzy, W.; Schmidt, R. R. Carbohydrate Res. 1987, J64, 265) was converted to the thio-donor 17' via a sequence of the iodo-sulfonamidation and subsequent rearrangements with ethanethiol in the presence of LiHMDS. Park, T.K., et al., J.Amer.Chem.Soc, 1996, 1 18, 1 1488. The MeOTf-promoted coupling to galactals 18' and 19' provided the trisaccharide glycals 20' and 21 ' in excellent yield and stereoselectivity. Reductive deprotection of the benzyl groups and the sulfonamide in 20' and subsequent uniform acetylation of the crude product yielded glycal 22'. The azidonitration of glycal 20'-22' provided intermediate azidonitrates, which were converted to the corresponding donors 23'-27'. The results of couplings of these trisaccharide donors with suitable serine/threonine derived acceptors are summarized in Table 6. The protection pattern again had a profound effect on the reactivity and stereoselectivity of the coupling. Despite the seemingly large distance between the hydroxyl and other functional groups of the lactose domain from the anomeric center, these substituents strongly affects the stereochemical outcome. Qualitatively, uniform protection of functionality with electron donating groups (cf. benzyl) leads to a very reactive donor by stabilizing the presumed oxonium cation. By contrast, electron withdrawing protecting groups tend to deactivate the donor in the coupling step. Andrews, C. W., et al., ). Org. Chem. 1996, 61, 5280; Halcomb, R. L; Danishefsky, S. J. /. Am. Chem. Soc. 1989, 1 1 1, 6656. Such deactivation may also confer upon a donor some stereochemical memory in terms of sensitivity of coupling to the original stereochemistry of the donor function at the anomeric center. As shown in Table 6, per-O-benzyl-protected donor 2W was highly reactive at -78°C providing product 28' in 90% yield and high stereoselectivity (10:1 , first entry, Table 6). A dramatic difference was seen upon changing the overall protection from per-O-benzyl to per-O-acetyl groups as demonstrated in the case of 5 donor 24'. The yield and stereoselectivity of the coupling step were diminished. Comparable results were obtained with donors 25' and 26'. In the case of compounds 27' and 28', where the galactosamine ring was conformationally restricted by engaging the 3- and 4-positions in the cyclic acetonide, an even more surprising finding was registered. Donor 27α' with a per-O-benzyl protected 10 lactosamine disaccharide afforded only the desired α-anomer 31 '. However, a mixture of trichloroacetimidates as well as the pure β anomer of 28' yielded undesired β anomer 32' exclusively. Thus, a modification of the protection pattern at a relatively distant site on the second and third carbohydrate units (from the ring containing the donor function) exerted a profound reversing effect on the stereoselectivity of glycosidation. Conformational 15 limitations imposed on a ring within the donor ensemble by cyclic protecting groups can influence donor reactivity, as judged by rates of hydrolysis. Wilson, B. G.; Fraser-Reid, B. ). Org. Chem. 1995, 60, 31 7; Fraser-Reid, B., et al., ). Am. Chem. Soc,. 1991, 1 13, 1434. Protecting groups, via their electronic, steric and conformational influences, coupled with solvation effects, can strongly modulate the characteristics of glycosyl donors. Thus, longer 20 range effects cannot be accurately predicted in advance in the glycosidation of serine and threonine side chain hydroxyls. Table 6. R, R 2 R 4 Catalyst/P romotor α:β(%) Bn Bn PhS0 2 HN 0(CNH)CCI 3 (23 ' α) Me TMSOTf (0.5eq), THF 10:1 (90%) 29 ' Ac Ac AcHN 0(CNH)CCI 3 (24 ' α β 3:1 ) Bn TMSOTf (1.Oeq), THF 2:1 (22%) 30 ' Ac Ac AcHN Br (25 O) Bn AgCIO, (1.5eq), CH 2 CI 2 3.5:1 (56%) 30 ' Ac Ac AcHN SPh (26 ' β) Bn NIS/TfOH, CH 2 CI 2 2:1 (40%) 30 ' Me 2 C Bn AcHN 0(CNH)CCI 3 (27 ' α) Bn TMSOTf (0.3eq), THF 1 :0 (50%) 31 ' Me 2 C Ac N, 0(CNH)CCI 3 (28 ' α/β 1 :1) Bn BF 3 Et 2 0 (0.5eq), THF 0:1 (67%) 32 ' Me 2 C Ac N, 0(CNH)CCI 3 (28 ' β) Bn BF 3 Et 2 0 (1.5eq), THF 0:1 (35%) 32 ' Accordingly, the present invention demonstrates unexpected advantages for the cassette approach wherein prebuilt stereospecifically synthesized α-O-linked serine or threonine

Claims: glycosides (e.g., 9' and 10') are employed to complete the saccharide assembly. What is Claimed Is: 1. A glycoconjugate having the structure: A-B m -C n -D p -E q -F wherein m, n, p and q are 0, 1 , 2 or 3 such that m + n + p + q _ 6; wherein A, B, C, D, E and F are independently amino acyl or hydroxy acyl residues wherein A is N- or O-terminal and is either a free amine or ammonium form when A is amino acyl or a free hydroxy when A is hydroxy acyl, or A is alkylated, arylated or acylated; wherein F is either a free carboxylic acid, primary carboxamide, mono- or dialkyl carboxamide, mono- or diarylcarboxamide, linear or branched chain (carboxy)alkyl carboxamide, linear or branched chain (alkoxycarbonyl)alkyl-carboxamide, linear or branched chain (carboxy)arylalkylcarboxamide, linear or branched chain (alkoxycarbonyl)alkylcarboxamide, an oligoester fragment comprising from 2 to about 20 hydroxy acyl residues, a peptidic fragment comprising from 2 to about 20 amino acyl residues, or a linear or branched chain alkyl or aryl carboxylic ester; wherein from one to about five of said amino acyl or hydroxy acyl residues are substituted by a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein the carbohydrate domain is linked to the respective amino acyl or hydroxy acyl residue by substitution of a side group substituent selected from the group consisting of OH, COOH and N H 2 ; wherein R 0 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R,, R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR 1 , N H 2 , NHCOR 1 , F, CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R„, R 12 , R 13 , R 14 and R, 5 are each independently hydrogen, OH, OR 1 ", NH 2 , NHCOR"', F, CH 2 OH, CH 2 OR'", or a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R, 6 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R" 1 is hydrogen, CHO, COOR lv , or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein R" and R' v are each independently H, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group. 2. The glycoconjugate of claim 1 wherein at least one carbohydrate domain has the oligosaccharide structure of a cell surface epitope. 3. The glycoconjugate of claim 2 wherein the epitope is Le a , Le , Le\ or Le y . The glycoconjugate of claim 2 wherein the epitope is MBrl , a truncated MBrl pentasaccharide or a truncated MBrl tetrasaccharide. 5. The glycoconjugate of claim 1 wherein the amino acyl residue is derived from a natural amino acid. 6. The glycoconjugate of claim 1 wherein at least one amino acyl residue has the formula: -NH-Ar-CO-. 7. The glycoconjugate of claim 6 wherein the Ar moiety is p-phenylene. 8. The glycoconjugate of claim 1 wherein at least one amino acyl or hydroxy acyl residue has the structure: wherein M, N and P are independently 0, 1 or 2; X is NH or O; Y is OH, NH or COOH; and wherein R' and R" are independently hydrogen, linear or branched chain alkyl or aryl . 9. The glycoconjugate of claim 1 wherein the amino acyl residue attached to a carbohydrate domain is Ser or Thr. 10. The glycoconjugate of claim 1 wherein one or more of R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R t0 , R,„ R 12 , R 13 , R 14 and R 15 is 1 R5,2RS,3-trihydroxy-propyl. 1 1. A pharmaceutical composition for treating cancer comprising a compound of claim 1 and a pharmaceutically suitable carrier. 12. A method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of a compound of claim 1 and a pharmaceutically suitable carrier. 13. The method of claim 12 wherein the cancer is a solid tumor. 14. The method of claim 12 wherein the cancer is an epithelial cancer. 15. A trisaccharide having the structure: wherein R„ R 3 , R 4 , R 5 , R 6 and R 7 are each independently hydrogen, OH, OR', NH 2 , NHCOR 1 , F, N 3 , CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is H, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; wherein R 2 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R 8 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R" is a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein X is a halide, a trihaloacetamidate, an alkyl or aryl sulfide or a dialkylphosphite. 16. The trisaccharide of claim 15 wherein X is a triethylphosphite. 17. The trisaccharide of claim 15 wherein R 7 is 1 RS,2RS,3-trihydroxypropyl or 1 R5,2R5,3-triacetoxypropyl. 18. The trisaccharide of claim 15 wherein R 8 is COOH. 19. A trisaccharide amino acid having the structure: wherein R,, R 3 , R 4 , R 5 , R 6 and R 7 are each independently hydrogen, OH, OR', NH 2 , NHCOR 1 , F, N 3 , CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R 1 is H, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; wherein R 2 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R 8 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R" is a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; wherein R 0 is a base-labile N-protecting group; and wherein R' is hydrogen or a lower alkyl group. 20. The trisaccharide amino acid of claim 19 wherein R 0 is FMOC. 21. A method of inducing antibodies in a human subject, wherein the antibodies are capable of specifically binding with human tumor cells, which comprises administering to the subject an amount of the glycoconjugate of claim 1 effective to induce the antibodies. 22. The method of claim 21 wherein the glycoconjugate is bound to a suitable carrier protein. 23. The method of claim 22 wherein the carrier protein is bovine serum albumin, polylysine or KLH. 24. The method of claim 21 which further comprises co-administering an immunological adjuvant. 25. The method of claim 24 wherein the adjuvant is bacteria or liposomes. 26. The method of claim 25 wherein the adjuvant is Salmonella minnesota cells, baciile Calmette-Guerin or QS21. 27. The method of claim 21 wherein the antibodies induced are selected from the group consisting of (2,6)-sialyl T antigen, Le a , Le b , Le", Le y , GM1 , SSEA-3 and MBrl antibodies. 28. The method of claim 21 wherein the subject is in clinical remission or, where the subject has been treated by surgery, has limited unresected disease. 29. A method of preventing recurrence of epithelial cancer in a subject which comprises vaccinating the subject with the glycoconjugate of claim 1 which amount is effective to induce antibodies. 30. The method of claim 29 wherein the glycoconjugate is bound to a suitable carrier protein. 31. The method of claim 30 wherein the carrier protein is bovine serum albumin, polylysine or KLH. 32. The method of claim 29 which further comprises co-administering an immunological adjuvant. 33. The method of claim 32 wherein the adjuvant is bacteria or liposomes. 34. The method of claim 32 wherein the adjuvant is Salmonella minnesota cells, baciile Calmette-Guerin or QS21. 35. The method of claim 29 wherein the antibodies induced are selected from the group consisting of (2,6)-sialyl T antigen, Le a , Le b , Le", Le y , GM1 , SSEA-3 and MBrl antibodies. 36. A glycoconjugate having the structure: wherein X is O or NR; wherein R is H, linear or branched chain alkyl or acyl; wherein A, B and C independently linear or branched chain alkyl or acyl, -CO-(CH 2 ) P - OH or aryl, or have the structure: wherein Y is O or NR; wherein D and E have the structure: -(CH 2 ) P -OH or -CO- (CH 2 ) P -OH; wherein N and P are independently an integer between 0 and 12; wherein D and E and, when any of A, B and C are -CO-(CH 2 ) P -OH, A, B and C are independently substituted by a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein the carbohydrate domain is linked to the respective hydroxy acyl residue by substitution of a terminal OH substituent; wherein R 0 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R β and R 9 are each independently hydrogen, OH, OR', N H 2 , NHCOR 1 , F, CH 2 OH, CH 2 OR', a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R n , R 12 , R 13 , R 14 and R 15 are each independently hydrogen, OH, OR 1 ", NH 2 , NHCOR'", F, CH 2 OH, CH 2 OR'", or a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R, 6 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R"' is hydrogen, CHO, COOR' v , or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein R" and R' v are each independently H, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group. 37. The glycoconjugate of claim 36 wherein at least one carbohydrate domain has the oligosaccharide structure of a cell surface epitope. 38. The glycoconjugate of claim 37 wherein the epitope is Le a , Le b , Le x , or Le y . 39. The glycoconjugate of claim 37 wherein the epitope is MBrl , a truncated MBrl pentasaccharide or a truncated MBrl tetrasaccharide. 40. The glycoconjugate of claim 36 wherein one or more of R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 R 9 , R 10 , R , R 12 , R 13 , R 14 and R ]5 is 1 R5,2R5,3-trihydroxy-propyl. 41. A pharmaceutical composition for treating cancer comprising a compound of claim 33 and a pharmaceutically suitable carrier. 42. A method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of a compound of claim 33 and a pharmaceutically suitable carrier. 43. The method of claim 42 wherein the cancer is a solid tumor. 44. The method of claim 42 wherein the cancer is an epithelial cancer. 45. A glycoconjugate comprising a core structure and a carbohydrate domain wherein the core structure is: wherein M is an integer from about 2 to about 5,000; wherein N is 1 , 2, 3 or 4; wherein A and B are suitable polymer termination groups; wherein the core structure is substituted by the carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein the carbohydrate domain is linked to the core structure by substitution of the OH substituents; wherein R 0 is hydrogen, a linear or branched chain alkyl, acyl, arylalkyi or aryl group; wherein R„ R 2 , R 3 , R 4 R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR 1 , NH 2 , NHCOR 1 , F, CH 2 OH, CHjOR 1 , a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R' is hydrogen, CHO, COOR", or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R,„ R 12 , R 13 , R, 4 and R 15 are each independently hydrogen, OH, OR'", N H 2 , NHCOR'", F, CH 2 OH, CH 2 OR'", or a substituted or unsubstituted linear or branched chain alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R )6 is hydrogen, COOH, COOR", CONHR", a substituted or unsubstituted linear or branched chain alkyl or aryl group; wherein R'" is hydrogen, CHO, COOR' v , or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group; and wherein R" and R' are each independently H, or a substituted or unsubstituted linear or branched chain alkyl, arylalkyi or aryl group. AMENDED CLAIMS [received by the International Bureau on 28 September 1998 (28.09.98); new claims 46-102 added; original claims unchanged (16 pages)] 46. A glycoconjugate having the structure: 4 wherein , n and p are integers between about 8 and about 20; wherein q is an 5 integer between about 1 and about 8; wherein Ry, R w , R x and R γ are independently 6 hydrogen, optionally substituted linear or branched chain lower alkyl or optionally 7 substituted phenyl; wherein R A , R 8 and R c are independently a carbohydrate domain 8 having the structure: erein R, is 14 hydrogen, linear or branched chain lower alkyl, acyl, arylalkyi or aryl group; wherein 15 R„ R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R„ and R 9 are each independently hydrogen, OH, OR, NH 2 , 16 NHCOR 1 , F, CH 2 OH, CH j OR 1 , an optionally substituted linear or branched chain 17 lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or trOacyioxyalkyl, arylalkyi 18 or aryl group; wherein R 1 is hydrogen, CHO, COOR 11 , or an optionally substituted 19 linear or branched chain lower alkyl, arylalkyi or aryl group or a saccharide moiety 20 having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R π , R 12 , R, 3 , R 14 and R 15 are each independently hydrogen, OH, OR", NH 2 , NHCOR'", F, CH 2 OH, CH 2 OR'", or an optionally substituted linear or branched chain lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R, 6 is hydrogen, COOH, COOR", CONHR", optionally substituted linear or branched chain lower alkyl or aryl group; wherein R'" is hydrogen, CHO, COOR' v , or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group; and wherein R" and R ιv are each independently hydrogen, or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group. 47. The glycoconjugate of claim 46 wherein Ry, R w , R x and R γ are methyl. 48. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently monosaccharides or disaccharides. 49. The glycoconjugate of claim 48 wherein y and z are 0; wherein x is 1 ; and wherein R 3 is NHAc. 50. The glycoconjugate of claim 46 wherein n is 0; wherein g and i are 1 ; wherein R, is OH; wherein R Q is hydrogen; and wherein R 8 is hydroxymethyl. 51. The glycoconjugate of claim 46 wherein m, n and p are 14; and wherein q is 3. 52. The glycoconjugate of claim 46 wherein each ammo acyl residue therein has an L- configuration. 53. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 54. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 55. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 56. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 57. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 58. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 59. The glycoconjugate of claim 46 wherein the carbohydrate domains are independently 60. A glycoconjugate having the structure: wherein the carrier is a protein; wherein the cross linker is a moiety derived from a cross linking reagent capable of conjugating a surface amine of the carrier and a thiol; wherein m, n and p are integers between about 8 and about 20; wherein / ' and q are independently integers between about 1 and about 8; wherein Ry, R w , R x and R γ are independently hydrogen, optionally substituted linear or branched chain lower alkyl or optionally substituted phenyl; wherein R A , R B and R c are independently a carbohydrate domain having the structure: wherein a, b, c, d, e, f, g, h, i, x, y and z are independently 0, 1 , 2 or 3; wherein Ro is hydrogen, linear or branched chain lower alkyl, acyl, arylalkyi or aryl group; wherein R,, R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently hydrogen, OH, OR, NH 2 , NHCOR 1 , F, CH 2 OH, CH 2 OR', an optionally substituted linear or branched chain lower alkyl, (mono-, di- or tri)hydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R 1 is hydrogen, CHO, COOR", or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group or a saccharide moiety having the structure: wherein Y and Z are independently NH or O; wherein k, I, r, s, t, u, v and w are each independently 0, 1 or 2; wherein R 10 , R„, R 12 , R 13 , R 14 and R, 5 are each independently hydrogen, OH, OR"', NH 2 , NHCOR"', F, CH 2 OH, CH 2 OR'", or an optionally substituted linear or branched chain lower alkyl, (mono-, di- or trijhydroxyalkyl, (mono-, di- or tri)acyloxyalkyl, arylalkyi or aryl group; wherein R, 6 is hydrogen, COOH, COOR", CONHR", optionally substituted linear or branched chain lower alkyl or aryl group; wherein R" is hydrogen, CHO, COOR' v , or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group; and wherein R" and R' are each independently hydrogen, or an optionally substituted linear or branched chain lower alkyl, arylalkyi or aryl group. 61 . The glycoconjugate of claim 60 having the structure: 62. The glycoconjugate of claim 60 wherein R j , R w , R x and R γ are methyl. 63. The glycoconjugate of claim 60 wherein the carbohydrate domains are monosacchaπdes or disacchaπdes. 64. The glycoconjugate of claim 63 wherein y and z are 0; wherein x is 1 ; and wherein 65. The glycoconjugate of claim 60 wherein h is 0; wherein g and / are 1 ; wherein R, is OH; wherein Ro is hydrogen; wherein m, n and p are 14; and wherein q is 3; and wherein R β is hydroxymethyl. 66. The glycoconjugate of claim 60 wherein the protein is BSA or KLH 67. The glycoconjugate of claim 60 wherein each ammo acyl residue therein has an L- configuration. 68. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 69. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 70. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 71. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 72. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 73. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 74. The glycoconjugate of claim 60 wherein the carbohydrate domains are independently 75. A pharmaceutical composition for treating cancer comprising a glycoconjugate of claim 46 or 60 and a pharmaceutically suitable carrier. 76. A method of treating cancer in a subject suffering therefrom comprising administering to the subject a therapeutically effective amount of a glycoconjugate of claim 46 or 60 and a pharmaceutically suitable carrier. 77. The method of claim 76 wherein the cancer is a solid tumor. 78. The method of claim 76 wherein the cancer is an epithelial cancer. 79. A method of inducing antibodies in a human subject, wherein the antibodies are capable of specifically binding with human tumor cells, which comprises administering to the subject an amount of the glycoconjugate of claim 46 or 60 effective to induce the antibodies. 80. The method of claim 79 wherein the carrier protein is bovine serum albumin, polylysine or KLH. 81. The method of claim 79 which further comprises co-administering an immunological adjuvant. 82. The method of claim 81 wherein the adjuvant is bacteria or liposomes. 83. The method of claim 81 wherein the adjuvant is Salmonella minnesota cells, baciile Calmette-Guerin or QS21. 84. The method of claim 79 wherein the antibodies induced are selected from the group consisting of Tn, ST N , (2,3)ST, glycophorine, 3^, 6-Le y , T(TF) and T antibodies. 85. The method of claim 79 wherein the subject is in clinical remission or, where the subject has been treated by surgery, has limited unresected disease. 86. A method of preventing recurrence of epithelial cancer in a subject which comprises vaccinating the subject with the glycoconjugate of claim 46 or 60 which amount is effective to induce antibodies. 87. The method of claim 86 wherein the carrier protein is bovine serum albumin, polylysine or KLH. 88. The method of claim 86 which further comprises co-administering an immunological adjuvant. 89. The method of claim 88 wherein the adjuvant is bacteria or liposomes. 90. The method of claim 88 wherein the adjuvant is Salmonella minnesota cells, baciile Calmette-Guerin or QS21. 91. The method of claim 86 wherein the antibodies induced are selected from the group consisting of Tn, ST N , (2,3)ST, glycophorine, 3-Le*, 6-Le y , T(TF) and T antibodies. 92. A method of preparing a protected O-linked Le. glycoconjugate having the structure: wherein R is hydrogen, linear or branched chain lower alkyl, or optionally substituted aryl; R, is t-butyloxycarbonyl, fluorenylmethyleneoxycarbonyl, linear or branched chain lower alkyl or acyl, optionally substituted benzyl or aryl; R 2 is a linear or branched chain lower alkyl, or optionally substituted benzyl or aryl; and R, is hydrogen, linear or branched chain lower alkyl or acyl, optionally substituted aryl or benzyl, or optionally substituted aryl sulfonyl; which comprises coupling a tetrasaccharide sulfide having the structure: wherein R 3 is linear or branched chain lower alkyl or aryl; with an O-linked glycosyl amino acyl component having the structure: under suitable conditions to form the protected O-linked Le. glycoconjugate. 93. The method of claim 92 wherein the tetrasaccharide sulfide is prepared by (a) halosulfoπamidating a tetrasaccharide glycal having the structure: under suitable conditions to form a tetrasaccharide halosulfonamidate; and (b) treating the halosulfonamidate with a mercaptan and a suitable base to form the tetrasaccharide sulfide. 94. The method of claim 92 wherein the mercaptan is a linear or branched chain lower alkyl or an aryl; and the base is sodium hydride, lithium hydride, potassium hydride, lithium diethylamide, lithium diisopropylamide, sodium amide, or lithium hexamethyldisilazide. 95. An O-linked glycoconjugate prepared in accord with claim 92. 96. An O-linked glycopeptide having the structure: wherein R 4 is a linear or branched chain lower acyl; and wherein R is hydrogen or a linear or branched chain lower alkyl or aryl. 97. The O-linked glycopeptide of claim 96 wherein R, is acetyl. 98. A method of preparing a protected O-linked Le^ glycoconjugate having the structure: wherein R is hydrogen, linear or branched chain lower alkyl, or optionally substituted aryl; R, is t-butyloxycarbonyl, fluorenylmethyleneoxycarbonyl, linear or branched chain lower alkyl or acyl, optionally substituted benzyl or aryl; and R 2 is a linear or branched chain lower alkyl, or optionally substituted benzyl or aryl; which comprises coupling a tetrasaccharide azidoimidate having the structure: with an O-linked glycosyl amino acyl component having the structure: under suitable conditions to form the protected O-linked Le^ glycoconjugate. 99. The method of claim 98 wherein the tetrasaccharide azidoimidate is prepared by (a) treating tetrasaccharide azidonitrate having the structure: under suitable conditions to form an azido alcohol; and (b) reacting the azido alcohol with an imidoacylating reagent under suitable conditions to form the azidoimidate. 100. The method of claim 99 wherein the tetrasaccharide azido nitrate is prepared by (a) converting a tetrasaccharide glycal having the structure: under suitable conditions to a peracetylated tetrasaccharide glycal having the structure: and (b) azidonitrating the glycal formed in step (a) under suitable conditions to form the tetrasaccharide azido nitrate. 101 . The method of claim 100 wherein step (b) is effected using cerium ammonium nitrate in the presence of an azide salt selected from the group consisting of sodium azide, lithium azide, potassium azide, tetramethylammonium azide and tetraethylammonium azide. 102. An O-linked glycoconjugate prepared in accord with claim 98.

Inventors: CHEN XIAO-TAO, DANISHEFSKY SAMUEL J, GLUNZ PETER, HINTERMANN SAMUEL, KUDUC SCOTT, LIVINGSTON PHILIP O, RAGUPATHI GOVINDASWAMI, SAMES DALIBOR, SCHWARZ JACOB B, CHEN, XIAO-TAO, DANISHEFSKY, SAMUEL, J., GLUNZ, PETER, HINTERMANN, SAMUEL, KUDUC, SCOTT, LIVINGSTON, PHILIP, O., RAGUPATHI, GOVINDASWAMI, SAMES, DALIBOR, SCHWARZ, JACOB, B.
Applicants: CHEN XIAO TAO, DANISHEFSKY SAMUEL J, GLUNZ PETER, HINTERMANN SAMUEL, KUDUC SCOTT, LIVINGSTON PHILIP O, RAGUPATHI GOVINDASWAMI, SAMES DALIBOR, SCHWARZ JACOB B, SLOAN KETTERING INST CANCER, CHEN, XIAO-TAO, DANISHEFSKY, SAMUEL, J., GLUNZ, PETER, HINTERMANN, SAMUEL, KUDUC, SCOTT, LIVINGSTON, PHILIP, O., RAGUPATHI, GOVINDASWAMI, SAMES, DALIBOR, SCHWARZ, JACOB, B., SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH
Application year: 1998
Main classification: A61K38
IPC codes: A61K38, A61K39, A61K45, A61P35, C07H3, C07H15, C07K7, C07K9, C07K14