FUSED THIOPHENE COMPOUNDS AND MEDICINAL USE THEREOF

Abstract: A condensed thiophene compound of the formula (I) wherein each symbol is as defined in the specification, a pharmaceutically acceptable salt thereof and hydrates thereof. The compound of the formula (I) of the present invention is useful as a novel antipsychotic agent which is effective for both positive symptoms and negative symptoms of schizophrenia, which is associated with less side effects such as extrapyramidal motor disorder and the like and which is less associated with serious side effects such as agranulocytosis and the like. In addition, this compound is also useful as a therapeutic agent of Alzheimer's disease and manic-depressive illness.

Description: Technical Field The present invention relates to condensed thiophene compounds useful for the treatment of schizophrenia, Alzheimer's disease, manic-depressive illness and the like, pharmaceutical use thereof and a synthetic intermediate thereof. Background Art Schizophrenia is a mental illness having a high incidence of about 1% of the entire population. In most cases, convalescence is inferior and patients and their families are forced to suffer from a great burden over a long period of time. To avoid this, many studies have been done as to the etiology of schizophrenia, development of therapeutic drugs thereof and the like. The first hypothesis proposed on the etiology of schizophrenia was an excess dopamine hypothesis. Based on this hypothesis, various compounds having a dopamine receptor inhibitory action have been developed as antipsychotic agents and achieved certain therapeutic effects. The conditions of schizophrenia include positive symptoms mainly showing delusion, hallucination and the like, negative symptoms mainly showing social withdrawal, emotional torpor and the like, recognition function disorder such as defects of memory, learning disability and the like, and the like. A so-called typical antipsychotic agent centering on inhibition of dopamine receptor is comparatively effective on positive symptoms but ineffective against negative symptoms and recognition function disorders. When a typical antipsychotic agent is used, extrapyramidal side effects (e.g., dystonia, akathisia, delayed dyskinesia and the like) are inevitably caused by the dopamine receptor inhibitory action. These difficulties suggest a limit on the development of antipsychotic agent based solely on a so-called excess dopamine hypothesis. In an attempt to solve the above-mentioned problems, antipsychotic agents, namely, serotonin-dopamine antagonist (SDA), having a serotonin receptor inhibitory action as a main action, have been studied and developed instead of dopamine receptor inhibitory action. Representative antipsychotic agents of SDA include risperidone, Seroquel and the like. However, the problems of poor effectiveness against negative symptoms and recognition function disorder or extrapyramidal side effects have not been entirely overcome [American Journal of Psychiatry 151, 825 (1994)]. One of the etiologic hypotheses of schizophrenia that have overtaken the excess dopamine hypothesis and the serotonin/dopamine hypothesis is a functional depression of glutamic acid nerve hypothesis [Trends in Neuroscience 13, 272 (1990)]. This hypothesis has been supported by the facts that (1) phencyclidine (PCP) which is an inhibitor of NMDA (N-methyl-D-aspartic acid) receptor induces mental conditions in human that are similar to schizophrenia with positive symptoms and negative symptoms [American Journal of Psychiatry 135, 1081 (1978), ibid. 148, 1301 (1991)], (2) cerebral cortex of schizophrenia patients shows lower reactivity of glutamic acid nervous system [Neuroscience Letters 121, 77 (1991)], (3) the number of NMDA receptors also present in the glutamic acid nervous system shows compensatory increase [Life Science 55, 1683 (1994)], (4) NMDA receptor agonists, such as glycine, D-cycloserine and the like, are effective for ameliorating the negative symptoms of schizophrenia [British Journal of Psychiatry 169, 610 (1996), American Journal of Psychiatry 152, 1213 (1995), ibid. 151, 1234 (1994)], and the like. Clozapine and olanzapine are atypical antipsychotic agents characterized by their effectiveness against positive symptoms as well as negative symptoms of schizophrenia [Psychopharmacology 63, 51 (1992), Neuropsychopharmacology 14, 111 (1996)]. These atypical antipsychotic agents suppress abnormal behaviors induced in test animals [Psychopharmacology 120, 67 (1995), ibid, 129, 79 (1997), Pharmacology, Biochemistry and Behavior 47, 579 (1994)] and abnormal physiological function [Psychopharmacology 111, 339 (1993), Journal of Pharmacology and Experimental Therapeutics 271, 787 (1994)] by the functional depression of the glutamic acid nervous system by NMDA receptor inhibitors such as PCP and MK-801 (dizocilpine maleate), and their inhibitory capability is frequently stronger than that of typical antipsychotic agents. In other words, the superior clinical effects of the atypical antipsychotic agent may be ascribed to amelioration of functional depression of glutamic acid nervous system in addition to conventional dopamine receptor inhibitory action and serotonin receptor inhibitory action. The neurophysiological function abnormalities induced by an NMDA receptor inhibitor includes NMDA receptor inhibitor-induced neurotoxicity [Archive of General Psychiatry 52,998 (1995)], and MK-801-induced neurotoxicity has been studied profoundly. This neurotoxic action can be inhibited by varioius antipsychotic agents, wherein the inhibitory action is stronger in clozapine and olanzapine that are atypical antipsychotic agents than in haloperidol and the like that are typical antipsychotic agents [Schizophrenia Research 15, 57 (1995), ibid. 21, 33 (1996)]. Using this MK-801-induced neurotoxic action as an index, an antipsychotic agent having an ameliorating action of functional depression of glutamic acid nervous system, that is one of the characteristics of clozapine and olanzapine, can be screened for. In addition, an MK-801-induced neurotoxic action is also considered a model of recognition function disorder observed in various diseases such as Alzheimer's disease and the like [Brain Research - Brain Research Reviews, 20, 250-267 (1995)], and a compound capable of inhibiting this neurotoxicity is also effective as a therapeutic drug of Alzheimer's disease, manic-depressive illness and the like. Meanwhile, the use of clozapine is limited, since it induces serious agranulocytosis, thougn it also shows superior antipsychotic action [New England Journal of Medicine 324, 746 (1991)]. Reduction of the possibility of side effects is also an important aspect in developing an antipsychotic agent. A report has been recently documented that a cation radical generated as a metabolitic intermediate of clozapine may be involved in the mechanism of onset of agranulocytosis [CNS• Drugs 7, 139 (1997)]. It is significant, therefore, to inhibit generation of cation radical to avoid the onset of agranulocytosis. Heretofore, there are various reports on suitable modification of the chemical structures of clozapine and olanzapine. For example, WO95/17400, WO96/18621, WO96/18623, WO96/18629, WO96/18630 and WO96/19479 disclose dibenzoxazepine compound, Japanese Patent Examined Publication Nos. 42-24513, 42-24514, 43-27404, 45-20909, 45-6822, 46-29861, 48-34599 and 49-40236, and Japanese Patent Unexamined Publication No. 47-4425 disclose dibenzoxazepine or dibenzothiazepine compound, WO93/07143 discloses pyridobenzoxazepine compound, Journal of Heterocyclic Chemistry 31, 1053 (1994) discloses thienobenzoxazepine compound, and Japanese Patent Unexamined Publication No. 63-8378 discloses dibenzothiazapine compound. In addition, benzothiophene compound is disclosed in, for example, Japanese Patent Unexamined Publication Nos. 52-87196 and 51-76296 with regard to 1,2,3,4-tetrahydrobenzothieno[2,3-b][1,5]benzodiazepine derivative. However, these compounds have remotely overcome the problems of effectiveness against negative symptoms and recognition function disorder, extrapyramidal side effects and the like, and activation of function of glutamic acid nervous system of the compounds of clozapine and olanzapine having suitably modified chemical structures has not been reported. Disclosure of the Invention The present inventors took note of the glutamic acid nervous functional depression hypothesis, and conducted intensive studies in an attempt to develop a compound which ameliorates positive symptoms as well as negative symptoms of schizophrenia and recognition function disorder, and which is free of serious side effects such as extrapyramidal disorders, agranulocytosis and the like. As a result, it has been found that the condensed thiophene compound of the formula (IA) has superior dopamine receptor inhibitory action and glutamic acid nervous system function depression-ameliorating action, shows anti-methamphetamine action, anti-apomorphine action, conditioned avoidance response inhibitory action, MK-801-induced neurotoxicity inhibitory action, MK-801 discrimination antagonistic action, clozapine discrimination generalization action and the like in test animals such as mouse, rat and the like, and is useful as an antipsychotic agent; that the compound shows less side effects in the extrapyramidal system, such as catalepsy induction and the like; and that a reaction intermediate corresponding to the cation radical intermediate considered to cause agranulocytosis induced by clozapine was not detected when the reaction intermediate was analyzed by an ESR spectrum, using oxydation of the compound of the formula (IA) by horseradish peroxidase as a model reaction of the metabolitic reaction of this compound, which resulted in the completion of the present invention. It is therefore an object of the present invention to provide a condensed thiophene compound useful as a therapeutic agent for schizophrenia and the like and an important synthetic intermediate therefor. The condensed thiophene compound of the formula (IA) is also useful as a therapeutic agent for Alzheimer's disease and manic-depressive illness. Accordingly, the present invention provides the following. A condensed thiophene compound of the formula (IA) wherein X is NH, or N-R 4 wherein R 4 is alkyl; R 1 and R 2 are the same or different and each is hydrogen, alkyl, alkoxy, hydroxyl or halogen; ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, cycloalkyl, alkoxy, alkoxyalkyl, halogen, alkyl halide, nitro, amino, monoalkylamino, dialkylamino, acylamino, hydroxyl and cyano or a benzene ring without a substituent; ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond; and R 3 is a group of the formula (1), formula (2), formula (3), formula (26), formula (27), formula (28), formula (29) or formula (30) in the formula (1), formula (26), formula (27) and formula (30), R 5 , R 6 and R 7 are the same or different and each is hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl or alkoxyalkyl and a is an integer of 2-4, in the formula (2) and formula (28), R 8 is hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, acyl, alkoxycarbonyl or alkoxyalkyl, b is 1 or 2, in the formula (3) and formula (29), R 9 is hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, acyl, alkoxycarbonyl or alkoxyalkyl, or a pharmaceutically acceptable salt thereof or a hydrate thereof. (3) The condensed thiophene compound of (2) above, wherein the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, or a pharmaceutically acceptable salt thereof or a hydrate thereof. (4) The condensed thiophene compound of (2) above, wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, the ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of allyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, and R 3 is a group of the formula (2) wherein R 8 is hydroxyalkoxyalkyl, methyl or ethyl and b is 1, or a pharmaceutically acceptable salt thereof or a hydrate thereof. (5) The condensed thiophene compound of (2) above, wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, the ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, and R 3 is a group of the formula (2) wherein R 8 is methyl or ethyl and b is 1, or a pharmaceutically acceptable salt thereof or a hydrate thereof. (6) The condensed thiophene compound of (2) above, which is a member selected from the group consisting of 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-methyl- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-chloro-12-[4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8,9-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b] [1,5]benzodiazepine, 8,10-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 2-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-2-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine 8,9-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 7,8-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-bromo-8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 1-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 4-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3,8-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-fluoro-8-methyl- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine and 12-(4-ethylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine or a pharmaceutically acceptable salt thereof or a hydrate thereof. (7) A pharmaceutical agent comprising a condensed thiophene compound of formula (IA) or a pharmaceutically acceptable salt thereof or a hydrate thereof, a pharmaceutical composition comprising said compound and a pharmaceutically acceptable additive, and an antipsychotic agent comprising, as an active ingredient, a compound of the formula (IA). (8) A benzothiophene compound of the formula (IIA) wherein X is NH or N-R 4 wherein R 4 is alkyl, R 1 and R 2 are the same or different and each is hydrogen, alkyl, alkoxy, hydroxyl or halogen, ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, cycloalkyl, alkoxy, alkoxyalkyl, halogen, alkyl halide, nitro, amino, monoalkylamino, dialkylamino, acylamino, hydroxyl and cyano or a benzene ring without a substituent, and ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, which is an important intermediate for the synthesis of the of compound of the formula (IA). (9) A benzothiophene compound of (8) above, wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, the ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen, hydroxyl and alkyl halide, or a benzene ring without a substituent, and the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond. (10) The benzothiophene compound of (8) above, which is a member selected from the group consisting of 6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-bromo-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-bromo-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8,9-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-3-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 2-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-2-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3,9-dimethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8,9-dichloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 7,8-dichloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-trifluoromethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-bromo-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 6-methyl-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 1-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 4-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3,8-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one and 3-fluoro-8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one. Detailed Description of the Invention In the present invention, each symbol used specifically means the following. Alkyl is alkyl having 1 to 5 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl and pentyl. Cycloalkyl is cycloalkyl having 3 to 6 carbon atoms, such as cyclopropyl, cyclopentyl, cyclohexyl and the like. Alkoxy is alkoxy having 1 to 5 carbon atoms, such as methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tert-butoxy and the like. Aryl is phenyl or naphthyl, with preference given to phenyl. Heteroaryl is pyridyl, furyl, thienyl and the like. Aralkyl is that wherein aryl has been substituted by alkyl having 1 to 5 carbon atoms and is exemplified by benzyl, 1-phenylethyl, 2-phenylethyl, 3-phenylpropyl, 4-phenylbutyl and the like. Hydroxyalkyl is hydroxyalkyl having 1 to 5 carbon atoms, such as hydroxymethyl, 2-hydroxyethyl, 1-hydroxyethyl, 3-hydroxypropyl, 1-hydroxypropyl, 4-hydroxybutyl, 1-hydroxy-1-methylethyl and the like. Hydroxyalkoxyalkyl is that wherein alkyl having 1 to 4 carbon atoms has been bonded to hydroxyalkyl having 1 to 4 carbon atoms via one oxygen atom, and is exemplified by 2-(2-hydroxyethoxy)ethyl and the like. Aminoalkyl is that wherein alkyl having 1 to 5 carbon atoms has been substituted by amino, which is exemplified by aminomethyl, 2-aminoethyl, 1-aminoethyl, 3-aminopropyl, 1-aminopropyl, 4-aminobutyl, 1-amino-1-methylethyl and the like. Monoalkylaminoalkyl is that wherein nitrogen atom of aminoalkyl is substituted by one alkyl having 1 to 5 carbon atoms, which is exemplified by methylaminomethyl, N-methyl-2-aminoethyl, N-methyl-1-aminoethyl, N-methyl-3-aminopropyl, N-methyl-1-aminopropyl, N-methyl-4-aminobutyl, N-methyl-1-amino-1-methylethyl and the like. Dialkylaminoalkyl is that wherein nitrogen atom of aminoalkyl has been substituted by two alkyl having 1 to 5 carbon atoms, which is exemplified by dimethylaminomethyl, N,N-dimethyl-2-aminoethyl, N,N-dimethyl-1-aminoethyl, N,N-dimethyl-3-aminopropyl, N,N-dimethyl-1-aminopropyl, N,N-dimethyl-4-aminobutyl, N,N-dimethyl-1-amino-1-methylethyl and the like. Alkoxyalkyl is that wherein alkyl having 1 to 5 carbon atoms has been substituted by alkoxy, such as methoxymethyl, methoxyethyl, ethoxymethyl, ethoxyethyl and the like. Halogen is fluorine, chlorine, bromine or iodine. Alkyl halide is that having 1 to 5 carbon atoms, such as fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, dichloromethyl, trichloromethyl and the like. Monoalkylamino is amino substituted by one alkyl having 1 to 5 carbon atoms, such as methylamino, ethylamino and the like. Dialkylamino is amino substituted by two alkyl having 1 to 5 carbon atoms, such as dimethylamino, diethylamino and the like. Acyl is that having 2 to 7 carbon atoms, such as acetyl, propanoyl, butanoyl, pivaloyl, benzoyl and the like. Acylamino is that wherein amino or monoalkylamino is bonded to acyl having 1 to 5 carbon atoms, such as acetylamino, N-acetyl-N-methylamino and the like. As used herein, acyl means that having 2 to 5 carbon atoms, such as acetyl, propanoyl, butanoyl, pivaloyl and the like. Alkoxycarbonyl is that having 2 to 8 carbon atoms, such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, butoxycarbonyl, tert-butoxycarbonyl, benzyloxycarbonyl, pentoxycarbonyl and the like. The group of the formula (1) as defined by the above-mentioned R 5 , R 6 , R 7 and integer a is N-(N',N'-dimethyl-2-aminoethyl)amino, N-(N',N'-dimethyl-2-aminoethyl)-N-methylamino and the like. The group of the formula (2) as defined by the above-mentioned R 8 and integer b is piperazin-1-yl, 4-methylpiperazin-1-yl, 4-ethylpiperazin-1-yl, 4-propylpiperazin-1-yl, 4-isopropylpiperazin-1-yl, 4-[2-(2-hydroxyethoxy]ethyl]piperazin-1-yl, 4-methylhomopiperazin-1-yl and the like, with preference given to 4-methylpiperazin-1-yl. The group of the formula (3) as defined by the above-mentioned R 9 is 8-methyl-3,8-diazabicyclo[3.2.1]octan-3-yl and the like. The group of the formula (26) as defined by the above-mentioned R 5 and R 6 is amino, N,N-dimethylamino and the like. The group of the formula (27) as defined by the above-mentioned R 5 , R 6 , R 7 and integer a is N-(N',N'-dimethyl-2-aminoethyl)amino-N'-oxide, N-(N',N'-dimethyl-2-aminoethyl)-N-methylamino-N'-oxide and the like. The group of the formula (28) as defined by the above-mentioned R 8 and integer b is 4-methylpiperazine-4-oxide-1-yl and the like. The group of the formula (29) as defined by the above-mentioned R 9 is 8-methyl-3,8-diazabicyclo[3.2.1]octane-8-oxide-3-yl and the like. The group of the formula (30) as defined by the above-mentioned R 5 and R 6 is amino-N-oxide, N,N-dimethylamino-N-oxide and the like. The compound of the formula (IA), a pharmaceutically acceptable salt thereof and hydrates thereof are preferably a condensed thiophene compound of the formula (IA) wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, and R 3 is a group of the formula (2) wherein R 8 is hydroxyalkoxyalkyl, methyl or ethyl and b is 1, a pharmaceutically acceptable salt thereof and hydrates thereof. More preferred are a condensed thiophene compound of the formula (IA) wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, and R 3 is a group of the formula (2) wherein R 8 is methyl or ethyl and b is 1, a pharmaceutically acceptable salt thereof and hydrates thereof. Preferable compounds of the formula (IA) include the following compounds wherein the number denotes Example numbers. (1) 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (2) 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (6) 8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (7) 8-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (8) 8-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (49) 9-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (10) 9-methyl- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (11) 9-chloro- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (12) 9-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (13) 9-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (50) 8,9-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (15) 8,10-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (16) 3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (17) 8-fluoro-3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (18) 2-methyl-12-[4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (19) 8-fluoro-2-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (20) 3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (21) 8-fluoro-3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b] [1,5]benzodiazepine, (22) 8,9-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (23) 7,8-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (25) 3-bromo-8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (42) 3-fluoro- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (43) 3-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (45) 1-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (46) 4-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (47) 3,8-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, (48) 3-fluoro-8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, and (51) 12-(4-ethylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine. The present invention also encompasses pharmaceutically acceptable salts of the above-mentioned compounds and hydrates thereof. The compound of the formula (IIA) is preferably a benzothiophene compound wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, and ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond. Specific compounds of the formula (IIA) include the following: 6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-bromo-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-bromo-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8,9-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-3-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 2-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-2-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3,9-dimethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8,9-dichloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 7,8-dichloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-trifluoromethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-bromo-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 6-methyl-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 1-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 4-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3,8-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one and 3-fluoro-8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one. The pharmaceutically acceptable salt of the compound of the formula (IA) includes salts with inorganic acid, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, and salts with organic acid, such as acetic acid, propionic acid,succinic acid, glycolic acid, lactic acid, malic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, camphorsulfonic acid, ascorbic acid, maleic acid, citric acid, tartaric acid, fumaric acid and the like. The compound of the formula (IA) and pharmaceutically acceptable salts thereof may exist in the form of hydrates or solvates, and their hydrates (1/2 hydrates, monohydrates, dihydrates, trihydrates and the like) and solvates are also encompassed in the present invention. When the inventive compounds include asymmetric atom, they can be obtained in the form of racemic mixture or optically active compound. When the inventive compounds include at least two asymmetric atoms, they can be obtained in the form of respective diastereomers or mixtures thereof. The present invention also encompasses these mixtures and individual isomers. The present invention further encompasses stereoisomers. The synthetic methods of the compounds of the present invention are as follows. Method 1: synthetic method of compound of formula (IA) A benzothiophene compound of the formula (IIA) wherein each symbol is as defined above is kept with a suitable chlorinating agent such as thionyl chloride, phosphorus oxychloride, phosphorus pentachloride and the like, in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, chloroform, 1,2-dichloroethane, an optionally mixed solvents therefrom and the like or without solvent, at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (4A) wherein each symbol is as defined above. The obtained compound of the formula (4A) is kept with a compound of the formula (5) H-R 3 wherein R 3 is as defined above, in a solvent that does not adversely affect the reaction such as benzene, toluene, xylene, chloroform, 1,2-dichloroethane, an optionally mixed solvents therefrom and the like or without solvent, at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (IA). Method 2: synthetic method of compound of formula (IA) A benzothiophene compound of the formula (IIA) is kept with a suitable sulfurating agent such as diphosphorus tetrasulfate, Lawesson reagent and the like in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, chloroform, 1,2-dichloroethane, an optionally mixed solvents therefrom and the like, or without solvent at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (7) wherein each symbol is as defined above. The obtained compound of the formula (7) is reacted with a suitable methylating agent such as methyl iodide, methyl bromide and the like in a solvent that does not adversely affect the reaction such as tetrahydrofuran, N,N-dimethylformamide, acetonitrile, N-methylpyrolidone, dichloromethane, chloroform, 1,2-dichloroethane, an optionally mixed solvents therefrom and the like, in the presence of an organic base such as pyridine, triethylamine, diisopropylethylamine and the like or an inorganic base such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium and the like, or without a base to give a compound of the formula (8) wherein each symbol is as defined above. The obtained compound of the formula (7) or the formula (8) is kept with a compound of the formula (5) in a solvent that does not adversely affect the reaction, such as tetrahydrofuran, N,N-dimethylformamide, acetonitrile, N-methylpyrolidone, dichloromethane, benzene, toluene, xylene, chloroform, 1,2-dichloroethane, an optionally mixed solvents therefrom and the like, or without solvent, at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (IA). Method 3: synthetic method of compound of formula (IA) wherein X is NH, N-R 4 , O or S A benzothiophene compound of the formula (9) wherein R 10 is alkyl, R 11 is halogen and other symbols are as defined above, is kept with a compound of the formula (10) wherein R 12 is NH 2 , NH-R 4 , OH or SH and ring A is as defined above, in a solvent that does not adversely affect the reaction, such as N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, ethanol, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base such as pyridine, triethylamine, diisopropylethylamine and the like or an inorganic base such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium and the like at -20°C to 170°C for 1 to 24 hr to give a compound of the formula (11) wherein each symbol is as defined above. A compound of the formula (11a) wherein R 13 is hydrogen or alkyl and other symbols are as defined above, which is a compound of the formula (11) wherein X is NH or N-R 4 , can be also synthesized by the following method. For example, a benzothiophene compound of the formula (12) wherein each symbol is as defined above, is kept with a compound of the formula (13) wherein R 14 is halogen and ring A is as defined above, in a solvent that does not adversely affect the reaction, such as N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, ethanol, 1,4-dioxane, an optionally mixed solvents therefrom and the like, or without solvent, in the presence of an organic base such as pyridine, triethylamine, diisopropylethylamine and the like or an inorganic base such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium and the like at -20°C to 170°C for 1 to 24 hr to give the above-mentioned compound of the formula (11a). In particular, of the compounds of the formula (11a), a compound wherein R 13 is alkyl can be also obtained by keeping a compound wherein R 13 is hydrogen with an alkyl halide of the formula (6) to be mentioned later, in a solvent that does not adversely affect the reaction, such as N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base such as pyridine, triethylamine, diisopropylethylamine and the like, or an inorganic base such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium, lithium diisopropylamine and the like at room temperature to 170°C for 1 to 24 hr. The obtained compound of the formula (11) is kept with hydrogen at normal pressure to 100 atm, in a solvent that does not adversely affect the reaction such as ethanol, methanol, ethyl acetate, dimethylformamide, acetic acid, water, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of a suitable metal catalyst such as palladium-carbon, palladium black, palladium hydroxide, Raney nickel, platinium oxide and the like at room temperature to 150°C for 1 to 24 hr to give a compound of the formula (14) wherein each symbol is as defined above. The compound of the formula (14) can be also obtained by keeping a compound of the formula (11) with a suitable inorganic reagent such as iron, zinc, tin(II) chloride and the like, in a solvent that does not adversely affect the reaction, such as ethanol, methanol, 1,4-dioxane, dimethylformamide, acetic acid, water, an optionally mixed solvent therefrom and the like, in the presence of a suitable acid such as hydrochloric acid, acetic acid and the lik at -20°C to 150°C for 1 to 24 hr. In addition, of the compounds of the formula (14), a compound wherein X is S or O can be also obtained by keeping a compound of the formula (9) with a compound of the formula (25) wherein R 16 is OH or SH and ring A is as defined above, in a solvent that does not adversely affect the reaction, such as N,N-dimethyformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, ethanol, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base such as pyridine, triethylamine, diisopropylethylamine and the like or an inorganic base such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium and the like at -20°C to 150°C for 1 to 24 hr. The obtained compound of the formula (14) is kept with a compound of the formula (5) in a solvent that does not adversely affect the reaction, such as anisole, toluene, xylene, mesitylene, nitrobenzene, 1,2-dichloroethane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of a suitable Lewis acid such as titanium tetrachloride, thin tetrachloride, aluminium chloride and the like at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (IA). Method 4: A compound of the formula (IAa) wherein each symbol is as defined above, which is a compound of the formula (IA) wherein X is N-R 4 , can be also obtained by keeping a benzothiophene compound of the formula (IAb) wherein each symbol is as defined above, with an alkyl halide of the formula (6) Hal-R 4 wherein Hal is halogen and R 4 is as defined above, in a solvent that does not adversely affect the reaction, such as N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base such as pyridine, triethylamine, diisopropylethylamine and the like, or an inorganic base such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium and the like at room temperature to 170°C for 1 to 24 hr. Method 5: A compound of the formula (IAc) wherein c is 1 or 2 and other symbols are as defined above, which is a compound of the formula (IA) wherein X is SO or SO 2 , can be also obtained by keeping a benzothiophene compound of the formula (IAd) wherein each symbol is as defined above, with an oxidizing agent such as hydrogen peroxide, m-chloroperbenzoic acid, sodium periodate, sodium perbromate and the like, in a solvent that does not adversely affect the reaction, such as water, ethanol, acetic acid, formic acid, dichloromethane, chloroform, an optionally mixed solvent therefrom and the like, or without solvent, at -78°C to 120°C for 1 to 24 hr. Method 6: synthetic method of compound of formula (IIA) A compound of the formula (14) is kept with an organic base, such as pyridine, triethylamine, diisopropylethylamine and the like, or an inorganic base, such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium and the like, or a suitable Lewis acid, such as titanium tetrachloride, thin tetrachloride, aluminium chloride, boron trifluoride and the like, or a suitable acid, such as sulfuric acid, phosphoric acid and the like, in a solvent that does not adversely affect the reaction, such as dichloromethane, chloroform, tetrahydrofuran, dimethyl sulfoxide, N,N-dimethylformamide, benzene, toluene, xylene, an optionally mixed solvent therefrom and the like, at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (IIA). A compound of the formula (14) is kept with a suitable inorganic base, such as sodium hydroxide, potassium hydroxide, barium hydroxide and the like, in a solvent that does not adversely affect the reaction, such as methanol, ethanol, water, tetrahydrofuran, 1,4-dioxane, an optionally mixed solvent therefrom and the like, at room temperature to 150°C for 1 to 24 hr to give a compound of the formula (15) wherein each symbol is as defined above. The obtained compound of the formula (15) is kept with a suitable condensing agent, such as dicyclohexylcarbodiimide, diisopropylcarbodiimide, N-ethyl-N'-3-dimethylaminopropylcarbodiimide, diethylphosphoryl azide, diphenylphosphoryl azide and the like, in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, hexane, ethyl acetate, diethyl ether, tetrahydrofuran, dioxane, chloroform, dichloromethane, 1,2-dichloroethane, N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, pyridine, an optionally mixed solvent therefrom and the like, at room temperature to 100°C for 10 min to 24 hr to give a compound of the formula (IIA). Method 7: synthetic method of compound of formula (IIA) A compound of the formula (16) wherein each symbol is as defined above, is kept with a suitable chlorinating agent such as thionyl chloride, phosphorus oxychloride, phosphorus pentachloride, oxalyl chloride and the like in a solvent that does not adversely affect the reaction such as benzene, toluene, xylene, chloroform, 1,2-dichloroethane, an optionally mixed solvent therefrom and the like, or without solvent, at room temperature to 170°C for 1 to 24 hr to give a compound of the formula (17) wherein each symbol is as defined above. The obtained compound of the formula (17) is kept with a compound of the formula (18) wherein each symbol is as defined above, in a solvent that does not adversely affect the reaction such as benzene, toluene, xylene, hexane, ethyl acetate, diethyl ether, tetrahydrofuran, dioxane, chloroform, dichloromethane, 1,2-dichloroethane, N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, pyridine, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base, such as pyridine, triethylamine, diisopropylethylamine and the like, or an inorganic base, such as potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide and the like at room temperature to 170°C for 10 min to 24 hr to give a compound of the formula (19) wherein each symbol is as defined above. A compound of the formula (19) can be also obtained by keeping a compound of the formula (16) with a compound of the formula (18) in a solvent that does not adversely affect the reaction such as benzene, toluene, xylene, hexane, ethyl acetate, diethyl ether, tetrahydrofuran, dioxane, chloroform, dichloromethane, 1,2-dichloroethane, N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, pyridine, an optionally mixed solvent therefrom and the like and a suitable condensing agent, such as dicyclohexylcarbodiimide, diisopropylcarbodiimide, N-ethyl-N'-3-dimethylaminopropylcarbodiimide, diethylphosphoryl azide, diphenylphosphoryl azide and the like at room temperature to 100°C for 10 min to 24 hr. The obtained compound of the formula (19) is kept in a solvent that does not adversely affect the reaction, such as methanol, ethanol, N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, hexamethylphosphorous triamide, benzene, toluene, xylene, chloroform, dichloroethane and the like, an optionally mixed solvent therefrom and the like, in the presence of an organic base, such as pyridine, triethylamine, N,N-diisopropylethylamine and the like, or an inorganic base, such as potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, sodium hydride and the like at room temperature to 170°C for 10 min to 24 hr to give a compound of the formula (IIA). Method 8: A compound of the formula (IIAa) wherein each symbol is as defined above, which is a compound of the formula (IIA) wherein X is SO or SO 2 , can be obtained by keeping a benzothiophene compound of the formula (IIAb) wherein each symbol is as defined above, with an oxidizing agent, such as hydrogen peroxide, m-chloroperbenzoic acid, sodium periodate, sodium perbromate and the like, in a solvent that does not adversely affect the reaction, such as water, ethanol, acetic acid, formic acid, dichloromethane, chloroform, an optionally mixed solvent therefrom and the like, or without solvent, at -78°C to 120°C for 1 to 24 hr. Method 9: A compound of the formula (IIAc) wherein each symbol is as defined above, which is a compound of formula (IIA) wherein X is N-R 4 , can be also obtained by, for example, keeping a benzothiophene compound of the formula (IIAd) wherein each symbol is as defined above, with an alkyl halide of the formula (6), in a solvent that does not adversely affect the reaction, such as N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base, such as pyridine, triethylamine, diisopropylethylamine and the like, or an inorganic base, such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium, lithium diisopropylamide and the like at room temperature to 170°C for 1 to 24 hr. Method 10: A compound of the formula (IIAe) wherein each symbol is as defined above provided that a compound wherein X is NH is excluded, which is a compound of formula (IIA) wherein ring B is a benzene ring, can be also obtained by keeping a 1,2,3,4-tetrahydrobenzothiophene compound of the formula (IIAf) wherein each symbol is as defined above, with a suitable oxidizing agent, such as sulfur, N-bromosuccinimide, N-chrolosuccinimide, N-iodosuccinimide, dichlorodicyano-p-benzoquinone, or a suitable noble metallic catalyst, such as palladium-carbon, palladium hydroxide, palladium black, platinium oxide and the like, in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, mesitylene, chloroform, carbon tetrachloride, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent at 0°C to 250°C for 1 to 48 hr. Method 11: A compound of the formula (IIAg) wherein each symbol is as defined above, which is a compound of formula (IIA) wherein substituent on ring A is amino, can be obtained by keeping a compound of the formula (IIAh) wherein each symbol is as defined above, which is a compound of formula (IIA) wherein a substituent on ring A is nitro, with hydrogen, in a solvent that does not adversely affect the reaction, such as methanol, ethanol, ethyl acetate, dioxane, benzene, toluene, xylene, diethyl ether, chloroform, 1,2-dichloroethane, an optionally mixed solvent therefrom and the like, using palladium-carbon, palladium hydroxide, platinium oxide and the like as a catalyst at 0°C to 100°C and from normal pressure to 100 atm for 10 min to 24 hr. Method 12: A compound of the formula (IIAi) wherein R 15 is fluorine, chlorine, bromine, iodine or cyano and other symbols are as defined above, which is a compound of formula (IIA) wherein substituent on ring A is cyano or halogen, can be obtained by diazotizing a compound of the formula (IIAg) in a solvent that does not adversely affect the reaction, such as water, diluted hydrochloric acid and the like and using sodium nitrite and the like at -10°C to room temperature and keeping with fluoroboric acid, hydrogen fluoride-pyridine, sodium chloride, cuprous chloride, sodium bromide, cuprous bromide, sodium iodide, potassium iodide and the like at -10°C to 100°C. Method 13: synthetic method of compound of formula (IA) wherein X is NH and bond shown by dotted line and solid line in B ring is double bond Using a benzylcyanide compound of the formula (20) wherein each symbol is as defined above, as a starting material and according to the method described in Japanese Patent Unexamined Publication No. 7-89965 to give a benzothiophene compound of the formula (21) wherein each symbol is as defined above. The obtained compound of the formula (21) is kept with a compound of the formula (13) in a solvent that does not adversely affect the reaction, such as N,N-dimethylformamide, dimethyl sulfoxide, N-methylpyrolidone, tetrahydrofuran, ethanol, 1,4-dioxane, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of an organic base, such as pyridine, triethylamine, diisopropylethylamine and the like, or inorganic base, such as sodium hydride, potassium carbonate, sodium carbonate, sodium hydroxide, potassium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium or the like at -50°C to 170°C for 1 to 24 hr to give a compound of the formula (22) wherein each symbol is as defined above. The obtained compound of the formula (22) is kept with a suitable reducing agent such as iron, zinc, tin(II) chloride and the like, in a solvent that does not adversely affect the reaction, such as ethanol, methanol, 1,4-dioxane, dimethylformamide, acetic acid, water, an optionally mixed solvent therefrom and the like, in the presence of a suitable acid, such as hydrochloric acid, sulfuric acid, nitric add, phosphoric acid, acetic acid and the like at -20°C to 150°C for 1 to 24 hr to give a compound of the formula (23) wherein each symbol is as defined above or a salt thereof with an inorganic acid, such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid and the like. A compound of the formula (23) can be obtained by keeping a compound of the formula (22) with hydrogen from normal pressure to 100 atm in a solvent that does not adversely affect the reaction, such as ethanol, methanol, ethyl acetate, dimethylformamide, acetic acid, water, an optionally mixed solvent therefrom and the like or without solvent, in the presence of a suitable metallic catalyst, such as palladium-carbon, palladium black, palladium hydroxide, Raney nickel, platinium oxide and the like at room temperature to 150°C for 1 to 24 hr, or by keeping a compound of the formula (24) wherein each symbol is as defined above, which is obtained by keeping with a suitable reducing agent, such as sodium hydrosulfite, iron, zinc, tin(II) chloride and the like in the presence of hydrochloric acid, acetic acid and the like, or under neutral conditions, at -20°C to 150°C for 1 to 24 hr is kept in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, dimethyl sulfoxide, N,N-dimethylformamide, 1,4-dioxane, ethanol, methanol, acetic acid, water, an optionally mixed solvent therefrom and the like, in the presence or absence of an suitable inorganic acid, such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid and the like, at room temperature to 180°C for 1 to 24 hr. The obtained compound of the formula (23) is kept with a compound of the formula (1), (2), (3), (26), (27), (28), (29) or (30) in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, dimethyl sulfoxide, N,N-dimethylformamide, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of a suitable organic base, such as pyridine, triethylamine, diisopropylethylamine and the like, or inorganic base, such as potassium carbonate, sodium carbonate and the like, or in the absence of a base at room temperature to 180°C for 1 to 20 hr to give a compound of the formula (IA) wherein X is NH and the bond shown by a dotted line and a solid line in the B ring is a double bond. Method 14: Compounds of formulas (IAe) and (IAf) wherein each symbol is as defined above, provided that when X is NH, the bond shown by a dotted line and a solid line is not a single bond, which are the compounds of the formula (IA) wherein R 3 is of formula (2) or (3) and R 8 or R 9 is hydrogen, can be also obtained by keeping a compound wherein R 3 is alkoxycarbonyl or acyl of the formula (2a), (2b), (3a) or (3b) wherein R 17 is alkyl or aralkyl, R 18 is alkyl or aryl and b is as defined above, in a solvent that does not adversely affect the reaction, such as ethanol, methanol, 1,4-dioxane, tetrahydrofuran, benzene, toluene, water, an optionally mixed solvent therefrom and the like or without using a solvent, in the presence of an inorganic acid such as hydrochloric acid, sulfuric acid and the like or an organic acid such as acetic acid, trifluoroacetic acid, p-toluenesulfonic acid and the like at 0°C to 150°C for 1 to 18 hr. The compounds (IAe) and (IAf) can be also obtained by keeping compounds of the formulas (2c) and (3c) wherein R 22 is aryl and b is as defined above, with hydrogen at normal pressure to 100 atm in a solvent that does not adversely affect the reaction, such as ethanol, methanol, ethyl acetate, dimethylformamide, acetic acid, water, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of a suitable metallic catalyst, such as palladium-carbon, palladium black, palladium hydroxide, Raney nickel, platinium oxide and the like at room temperature to 150°C for 1 to 24 hr. Method 15: Compounds of formulas (IAg) and (IAh) wherein R 8' and R 9' are each acyl or alkoxycarbonyl and other symbols are as defined above, provided that when X is NH and the bond shown by a dotted line and a solid line is not a single bond, which are the compounds of the formula (IA) wherein R 3 is formula (2) or (3) and R 8 or R 9 is acyl or alkoxycarbonyl, can be obtained by keeping compounds (IAe) and (IAf) (R 8 or R 9 is hydrogen) with a suitable acylating agent, such as acyl halide of the formula (31) Hal-CO-R 19 wherein Hal is halogen and R 19 is alkyl or aryl, or acid anhydride of the formula (32) (R 19 CO) 2 O wherein R 19 is as defined above, or ester halide of the formula (33) Hal-COOR 20 wherein Hal is halogen and R 20 is alkyl or aralkyl as an alkoxycarbonylating agent, in a solvent that does not adversely affect the reaction such as benzene, toluene, xylene, chloroform, 1,4-dioxane, tetrahydrofuran, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of a basic reagent, such as pyridine, triethylamine, N, N-dimethylaminopyridine, sodium hydride, potassium carbonate, sodium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium, lithium diisopropylamine and the like, at -50 to 150°C for 10 min to 15 hr. Method 16: Compounds of formulas (IAi) and (IAj) wherein R 8 " and R 9 " are each alkyl, cycloalkyl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl or alkoxyalkyl and other symbols are as defined above, provided that when X is NH, the bond shown by a dotted line and a solid line is not a single bond, which are the compounds of the formula (IA) wherein R 3 is of formula (2) or (3) and R 8 or R 9 is alkyl, cycloalkyl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl or alkoxyalkyl, can be obtained by keeping compounds (IAe) and (IAf) (R 8 or R 9 is hydrogen) with a suitable alkylating agent, such as compound of the formula (34) Hal-R 21 wherein Hal is halogen and R 21 is alkyl, cycloalkyl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl or alkoxyalkyl in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, chloroform, 1,4-dioxane, tetrahydrofuran, an optionally mixed solvent therefrom and the like, or without solvent, in the presence of a suitable basic reagent such as pyridine, triethylamine, N,N-dimethylaminopyridine, sodium hydride, potassium carbonate, sodium hydroxide, potassium tert-butoxide, sodium alkoxide, butyllithium, lithium diisopropylamine and the like at -50 to 200°C for 10 min to 24 hr. Method 17: Of the compounds of the formula (IA), a compound wherein R 3 is of the formula (27), (28), (29) or (30) can be obtained by keeping the corresponding compound (1), (2), (3) or (26) with a suitable oxidizing agent, such as aqueous hydrogen peroxide, m-chloroperbenzoic acid, tert-butyl hydroperoxide and the like in a solvent that does not adversely affect the reaction such as methylene chloride, chloroform, 1,2-dimethoxyethane, benzene, toluene, diethyl ether, tetrahydrofuran, 1,4-dioxane, methanol, ethanol, water, an optionally mixed solvent therefrom and the like at -30 to 130°C for 10 min to 12 hr. Method 18: A compound of the formula (IIA) wherein X is O, S, SO or SO 2 and the bond shown by a dotted line and a solid line in the B ring is a double bond can be also obtained by keeping a compound of the formula (IIA) wherein X is O, S, SO or SO 2 and the bond shown by a dotted line and a solid line in the B ring is a single bond in a solvent that does not adversely affect the reaction, such as benzene, toluene, xylene, mesitylene, dichloromethane, chloroform, ethyl acetate, methanol, ethanol, tetrahydrofuran, 1,4-dioxane, dimethyl sulfoxide, N,N-dimethylformamide, an optionally mixed solvent therefrom and the like, in the presence of a suitable oxidizing agent, such as 2,3-dichloro-5,6-dicyano-1,4-benzoquinone, chloranil, manganese dioxide, nickel peroxide, palladium-carbon, lead tetraacetate and the like at room temperature to 200°C for 1 to 24 hr. Method 19: synthetic method of compound of formula (IB) When the compound obtained according to the above-mentioned method is to be purified as a salt of inorganic acid or organic acid, the following steps are taken. A compound of the formula (IA) is dissolved in a suitable solvent, such as methanol, ethanol, isopropyl alcohol, ethyl acetate, diethyl ether, diisopropyl ether, benzene, toluene, xylene, chloroform, methylene chloride and the like, an optionally mixed solvent therefrom and the like, and inorganic acid such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like or organic acid such as acetic acid, propionic acid, succinic acid, glycolic acid, lactic acid, malic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, camphorsulfonic acid, ascorbic acid, maleic acid, citric acid, tartaric acid, fumaric acid and the like or a hydrate thereof is added. The resulting crystals were recrystallized from a suitable solvent, such as methanol, ethanol, isopropyl alcohol, ethyl acetate, diethyl ether, diisopropyl ether, benzene, toluene, xylene, chloroform, methylene chloride, dichloroethane and the like or a mixed solvent thereof to give a salt of an inorganic acid, organic acid, hydrate or solvate of a compound of the formula (IA). The obtained compound of the present invention can be purified by one or more methods selected from crystallization, chromatography, extraction and filtration. When the resulting purified compound is a racemate, a desired optically active compound can be separated by, for example, preparative recrystallization from an optically active acid, or by passing the compound through a column packed with optically active carrier. The stereoisomer can be isolated by recrystallization, column chromatography and the like. The compound of the formula (IA), of the present invention obtained in the above-mentioned manner is useful as a novel antipsychotic agent which is effective for both the positive and negative symptoms of schizophrenia, which causes less side effects of extrapyramidal motor disorder and the like and which causes less serious side effects such as agranulocytosis and the like. The present invention compound (IA), is useful as a therapeutic agent for Alzheimer's disease and manic-depressive illness. The compounds of the formulas (IIA) are useful as important synthetic intermediates for the compounds of the formulas (IA). When the compound of the formula (IA) is used as a pharmaceutical agent, the inventive compound is admixed with a pharmaceutically acceptable carrier (e.g., excipient, binder, disintegrator, corrective, corrigent, emulsifier), diluent, solubilizer and the like to give a pharmaceutical composition by a conventional method, which is formulated into tablet, capsule, granule, powder, syrup, suspension, solution, injection, infusion, deposit agent, suppository and administered orally or parenterally. When the tablets are used for oral administration, typically used carriers include sucrose, lactose, mannitol, maltitol, dextran, and corn starch, typical lubricants such as magnesium stearate, preservatives such as parabens and, sorbins, antioxidants such as ascorbic acid, α-tocopherol and cystein, disintegrators and binders. When it is administered orally as capsules, effective diluents include lactose and dry corn starch. A liquid for oral use includes syrup, suspension, solution, emulsion and the like, which may contain typical inert diluent used in this field, such as water. In addition, sweeteners or flavors may be contained. In the case of parenteral administration such as subcutaneous injection, intravenous injection, intramuscular injection, intraperitoneal injection, infusion and the like, the pH of the active ingredient solution is adequately adjusted, bufferized and sterilized. Examples of usable vehicle or solvent include distilled water, Ringer solution and isotonic brine. For intravenous use, the total concentration of solute is adjusted to make the solution isotonic. Suppositories can be produced by admixing with a drug and a suitable nonirritative excipient such as those that are solid at normal temperature but become liquid at the temperature in the intestine and melt in rectum to release the drug, such as cocoa butter and polyethylene glycols. The dose is determined depending on age, body weight, administration time, administration method, combination of drugs, the level of condition for which a patient is undergoing therapy, and other factors. The compound of the present invention, optical isomers thereof and pharmaceutically acceptable salts thereof are low toxic and can be used safely. While the daily dose varies depending on the conditions and body weight of patients, the kind of compound, administration route and the like, in the case of oral use, it is about 0.01-300 mg/person/day, preferably 0.1-100 mg/person/day, in the case of parenteral use, it is desirably about 0.01-50 mg/person/day, preferably 0.01-10 mg/person/day for subcutaneous injection, intravenous injection, intramuscular injection and intrarectal injection. The present invention is explained in more detail in the following by way of Starting Material Synthesis Examples, Examples and Experimental Examples. The present invention is explained in more detail in the following by way of Starting Material Synthesis Examples, Examples and Experimental Examples . Starting Material Synthesis Example 1 Using cyclohexanone as a starting material and following the method disclosed in a publication [Archiv der Pharmazie (Weinheim), 317, 675 (1984)], ethyl 2-aminobenzo[b]thiophene-3-carboxylate was synthesized. 1 H-NMR (400MHz, CDCl 3 ) δ: 8.10(d, J=7.3Hz, 1H), 7.50(d, J=7.3Hz, 1H), 7.31(t, J=7.3Hz, 1H), 7.13(t, J=7.3Hz, 1H), 6.53(br.s, 2H), 4.42(q, J=7.3Hz, 2H), 1.46(t, J=7.3Hz, 3H) Starting Material Synthesis Example 2 Using 3-methylcyclohexanone as a starting material and following the method disclosed in a publication [Archiv der Pharmazie (Weinheim), 317, 675 (1984)], methyl 2-amino-5-methylbenzo[b]thiophene-3-carboxylate was synthesized. 1 H-NMR (400MHz, CDCl 3 ) δ: 7.89(s, 1H), 7.37(d, J=8.3Hz, 1H), 6.97(d, J=8.3Hz, 1H), 6.49(br.s, 2H), 3.95(s, 3H), 2.43(s, 3H) Starting Material Synthesis Example 3 Using 4-methylcyclohexanone as a starting material and following the method disclosed in a publication [Archiv der Pharmazie (Weinheim), 317, 675 (1984)], ethyl 2-amino-6-methylbenzo[b]thiophene-3-carboxylate was synthesized. melting point: 126-128°C Starting Material Synthesis Example 4 Ethyl 2-aminobenzo[b]thiophene-3-carboxylate (5 g) and 2-fluoronitrobenzene (2 ml) were dissolved in N,N-dimethylformamide (150 ml) and heated to 60°C. Thereto was added potassium carbonate (8 g) and the mixture was stirred at 100°C for 18 hr. After cooling, the reaction mixture was poured into ice water, and the precipitated crystals were collected by filtration. The crystals were washed with water and diisopropyl ether and dried at 60°C to give ethyl 2-(2-nitroanilino)-benzo[b]thiophene-3-carboxylate (5.7 g). 1 H-NMR (400MHz, CDCl 3 ) δ: 12.2(s, 1H), 8.32(d, J=8.3Hz, 1H), 8.25(d, J=8.3Hz, 1H), 8.10(d, J=8.8Hz, 1H), 7.67-7.62(m, 2H), 7.42(t, J=7.8Hz, 1H), 7.27(m, 1H), 7.16(t, J=7.8Hz, 1H), 4.57(q, J=7.3Hz, 2H), 1.52(t, J=7.3Hz, 3H) Starting Material Synthesis Example 5 Ethyl 2-aminobenzo[b]thiophene-3-carboxylate (69.3 g) and 2,4-difluoronitrobenzene (50 g) were dissolved in dimethyl sulfoxide (550 ml) and heated to 50°C. Thereto was added potassium carbonate (63 g) and the mixture was stirred at 100°C for 70 min. After cooling, the reaction mixture was allowed to cool to 70°C and poured into water (2.5 L). The reaction mixture was allowed to stand still, and filtered. The filtrated orange crystals were washed with ethyl acetate to give ethyl 2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate (50.7 g). melting point 137-140°C. 1 H-NMR (400MHz, CDCl 3 ) δ: 12.40(br.s, 1H), 8.33-8.29(m, 2H), 7.79(dd, J=7.8, 2.9 Hz, 1H), 7.65(d, J=8.3Hz, 1H), 7.42(dd, J=8.3, 8.2Hz, 1H), 7.29(dd, J=6.9, 8.3Hz, 1H), 6.80-6.77(m, 1H), 4.54(q, J=6.8Hz, 2H), 1.49(t, J=6.8Hz, 3H). IR (KBr): 3085, 2993, 1624, 1582, 1553, 1510, 1274, 1206, 1035 cm -1 . MS: m/e 360. Starting Material Synthesis Example 6 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (4.0 g), 2,4-dichloronitrobenzene (3.8 g) and dimethyl sulfoxide (55 ml), ethyl 2-(5-chloro-2-nitroanilino)benzo[b]thiophene-3-carboxylate (3.2 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 12.27(br.s, 1H), 8.31(d, J=8.3Hz, 1H), 8.19(d, J=8.8Hz, 1H), 8.08(s, 1H), 7.65(d, J=7.3Hz, 1H), 7.41(dd, J=7.3, 8.3Hz, 1H), 7.28(dd, J=7.8, 7.3Hz, 1H), 7.06(d, 8.3Hz, 1H), 4.54(q, J=7.3Hz, 2H), 1.49(t, J=7.3Hz, 3H) Starting Material Synthesis Example 7 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate, 2,4-dibromonitrobenzene and dimethyl sulfoxide, ethyl 2-(5-bromo-2-nitroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 8 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate, 2,5-difluoronitrobenzene and dimethyl sulfoxide, ethyl 2-(4-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 9 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (4.0 g), 4-fluoro-3-nitrotoluene (3.3 g) and dimethyl sulfoxide (55 ml), ethyl 2-(4-methyl-2-nitroanilino)benzo[b]thiophene-3-carboxylate (6.5 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 12.06(br.s, 1H), 8.27(d, J=8.3Hz, 1H), 8.01(s, 1H), 7.95(d, J=8.8Hz, 1H), 7.58(d, J=7.3Hz, 1H), 7 .44(d, J=8.3Hz, 1H), 7.38(dd, J=8.8, 8.3Hz, 1H), 7.21(d, J=7.3Hz, 1H), 4.53(q, J=7.3Hz, 2H), 2.40(s, 3H), 1.49(t, J=7.3Hz, 3H) Starting Material Synthesis Example 10 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate, 2,4,5-trifluoronitrobenzene and dimethyl sulfoxide, ethyl 2-(4,5-difluoro-2-nitroanilio)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 11 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate, 2,4,6-trifluoronitrobenzene and dimethyl sulfoxide, ethyl 2-(3,5-difluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 12 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate, 2-fluoro-5-methoxynitrobenzene and dimethyl sulfoxide, ethyl 2-(4-methoxy-2-nitroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 13 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-amino-6-methylbenzo[b]thiophene-3-carboxylate (4.5 g), 2,4-difluoronitrobenzene (3.1 g) and dimethyl sulfoxide, ethyl 2-(5-fluoro-2-nitroanilino)-6-methylbenzo[b]thiophene-3-carboxylate (5.1 g) was obtained. melting point:147-149°C Starting Material Synthesis Example 14 In the same manner as in Starting Material Synthesis Example 4 and using methyl 2-amino-5-methylbenzo[b]thiophene-3-carboxylate, 2-fluoronitrobenzene and dimethyl sulfoxide, methyl 2-(2-nitroanilino)-5-methylbenzo[b]thiophene-3-carboxylate was obtained. Starting Material Synthesis Example 15 In the same manner as in Starting Material Synthesis Example 4 and using methyl 2-amino-5-methylbenzo[b]thiophene-3-carboxylate (3.11 g), 2,4-difluoronitrobenzene (1.54 ml) and dimethyl sulfoxide (30 ml), methyl 2-(5-fluoro-2-mtroanilino)-5-methylbenzo[b]thiophene-3-carboxylate was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 12.44(br.s, 1H), 8.34(dd, J=9.3, 3.4Hz, 1H), 8.14(s, 1H), 7.82(d, J=8.3Hz, 1H), 7.55(d, J=7.8Hz, 1H), 7.14(d, J=8.3Hz, 1H), 6.83-6.78(m, 1H), 4.09(s, 3H), 2.49(s, 3H) Starting Material Synthesis Example 16 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-amino-6-methoxybenzo[b]thiophene-3-carboxylate, 2-fluoronitrobenzene and dimethyl sulfoxide, ethyl 6-methoxy-2-(2-nitroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 17 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-amino-6-methoxybenzo[b]thiophene-3-carboxylate, 2,4-difluoronitrobenzene and dimethyl sulfoxide, ethyl 2-(5-fluoro-2-nitroanilino)-6-methoxybenzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 18 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 6-bromo-2-aminobenzo[b]thiophene-3-carboxylate (8.8 g), 2,4-difluoronitrobenzene (5.6 g) and dimethyl sulfoxide (100 ml), ethyl 6-bromo-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate (10 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 12.35(s, 1H), 8.31(dd, 1H), 8.17(d, J=8.8Hz, 1H), 7.76-7.71(m, 2H), 7.50(d, J=8.8Hz, 1H), 6.82(dd, 1H), 4.53(q, J=7.3Hz, 2H), 1.48(t, J=7.3Hz, 3H) Starting Material Synthesis Example 19 Ethyl 2-(2-nitroanilino)benzo[b]thiophene-3-carboxylate (4.9 g) was dissolved in ethyl acetate (200 ml) and 10% palladium-carbon (0.5 g) was added. The mixture was stirred with hydrogen (60 kg/cm 2 ) at 60°C for 5 hr. The reaction mixture was cooled and the catalyst was filtered off. The filtrate was evaporated under reduced pressure to give ethyl 2-(2-aminoanilino)benzo[b]thiophene-3-carboxylate (3.5 g). 1 H-NMR (400MHz, CDCl 3 ) δ: 9.61(s, 1H), 8.14(d, J=7.8Hz, 1H), 7.47(d, J=7.8Hz, 1H), 7.33-7.35(m, 2H), 7.10-7.12(m, 2H), 6.80-6.86(m, 2H), 4.47(q, J=7.3Hz, 2H), 3.88(br.s, 2H), 1.50(t, J=7.3Hz, 3H) Starting Material Synthesis Example 20 Ethyl 2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate (50.7 g) was dissolved in ethyl acetate (1000 ml) and 10% palladium-carbon (8.0 g) was added. The mixture was stirred with hydrogen (60 kg/cm 2 ) at 55°C for 5 hr. The reaction mixture was cooled and the catalyst was filtered off. The filtrate was evaporated under reduced pressure and the residue was purified by silica gel column chromatography (chloroform) to give ethyl 2-(2-amino-5-fluoroanilino)benzo[b]thiophene-3-carboxylate (24.5 g). melting point 141-142°C. 1 H-NMR (400MHz, CDCl 3 ) δ: 9.87(s, 1H), 8.14(d, J=8.8Hz, 1H), 7.50(d, J=7.3Hz, 1H), 7.31-7.35(m, 1H), 7.19-7.11(m, 2H), 6.75-6.85(m, 2H), 3.69(br.s, 2H), 4.45(q, J=7.3Hz, 2H), 1.48(t, J=7.3Hz, 3H). IR (KBr): 3417, 3353, 3234, 2994, 1633, 1605, 1551, 1479, 1384, 1235, 1039 cm -1 . MS: m/e 330 Starting Material Synthesis Example 21 Ethyl 2-(5-chloro-2-nitroanilino)benzo[b]thiophene-3-carboxylate (3.2 g) was suspended in ethanol (40 ml) and a solution obtained by dissolving tin(II) chloride dihydrate (7.7 g) in ethanol (50 ml) and 18% hydrochloric acid (50 ml) was added with stirring the mixture. The mixture was stirred at 60°C for 2 hr and cooled to 30°C. Potassium carbonate was added until the reaction mixture was basified. After filtering through celite, the filtrate was extracted with ethyl acetate, washed with water and dried over magnesium sulfate. After natural filtration, the solvent was evaporated under reduced pressure to give ethyl 2-(2-amino-5-chloroanilino)benzo[b]thiophene-3-carboxylate (2.6 g). 1 H-NMR (400MHz, CDCl 3 ) δ: 9.71(s, 1H), 8.14(d, J=8.3Hz, 1H), 7.51(d, J=7.3Hz, 1H), 7.38(s, 1H), 7.36(t, J=7.3Hz, 1H), 7.15(dd, J=7.8, 7.3Hz, 1H), 7.09(d, J=7.8Hz, 1H), 6.77(d, J=8.3Hz, 1H), 4.47(q, J=7.3Hz, 2H), 3.88(br.s, 2H), 1.50(t, J=7.3Hz, 3H) Starting Material Synthesis Example 22 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(5-chloro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-5-chloroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 23 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(5-bromo-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-5-bromoanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 24 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4-fluoroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 25 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4-methyl-2-nitroanilino)benzo[b]thiophene-3-carboxylate (6.5 g), ethyl acetate (140 ml), 10% palladium-carbon and hydrogen (500 mg, 60 atm kg/cm 2 ), ethyl 2-(2-amino-4-methylanilino)benzo[b]thiophene-3-carboxylate (3.7 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 9.46(br.s, 1H), 8.12(d, J=8.3Hz, 1H), 7.45(d, J=7.3Hz, 1H), 7.33(dd, J=7.3, 7,8Hz, 1H), 7.18(d, J=8.3Hz, 1H), 7.10(dd, J=7.8, 8.3Hz, 1H), 6.67(s, 1H), 6.63(d, J=7.8Hz, 1H), 4.46(q, J=7.3Hz, 2H), 3.83(br.s, 2H), 1.56(s, 3H), 1.26(t, J=7.3Hz, 3H) Starting Material Synthesis Example 26 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4,5-difluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4,5-difluoroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 27 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(3,5-difluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-3,5-difluoroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 28 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4-methoxy-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4-methoxyanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 29 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(5-fluoro-2-nitroanilino)-6-methylbenzo[b]thiophene-3-carboxylate (1.9 g), ethyl acetate (200 ml), 10% palladium-carbon (0.4 g) and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-5-fluoroanilino)-6-methylbenzo[b]thiophene-3-carboxylate (1.54 g) was obtained. melting point: 174-175°C Starting Material Synthesis Example 30 In the same manner as in Starting Material Synthesis Example 19 and using methyl 2-(2-nitroanilino)-5-methylbenzo[b]thiophene-3-carboxylate, ethyl acetate, 10%palladium-carbon and hydrogen (60 atm kg/cm 2 ), methyl 2-(2-aminoanilino)-5-methylbenzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 31 In the same manner as in Starting Material Synthesis Example 19 and using methyl 2-(5-fluoro-2-nitroanilino)-5-methylbenzo[b]thiophene-3-carboxylate (4.42 g), ethyl acetate (400 ml), 10% palladium-carbon and hydrogen (400 mg, 60 atm kg/cm 2 ), methyl 2-(2-ammo-5-fluoroanilino)-5-methylbenzo[b]thiophene-3-carboxylate (1.82 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 9.84(s, 1H), 7.94(s, 1H), 7.40(d, J=7.8Hz, 1H), 7.19(d, J=9.3Hz, 1H), 6.98(d, J=8.3Hz, 1H), 6.86-6.77(m, 2H), 4.01(s, 3H), 3.69(br.s, 2H), 2.45(s, 3H) Starting Material Synthesis Example 32 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 6-methoxy-2-(2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-aminoanilino)-6-methoxybenzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 33 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-[5-fluoro-2-nitroanilino)-6-methoxybenzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-5-fluoroanilino)-6-methoxybenzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 34 In the same manner as in Starting Material Synthesis Example 21 and using ethyl 6-bromo-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate (10 g), ethanol (80 ml), 18% hydrochloric acid (80 ml) and tin(II) chloride•dihydrate (20.6 g), ethyl 6-bromo-2-(2-ammo-5-fluoroanilino)benzo[b]thiophene-3-carboxylate (3.8 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 9.81(s, 1H), 7.98(d, J=8.8Hz, 1H), 7.60(s, 1H), 7.41(d, J=8.8Hz, 1H), 7.13(d, J=9.3Hz, 1H), 6.84-6.76(m, 2H), 4.44(q, J=7.3Hz, 2H), 3.75(br.s, 2H), 1.47(t, J=7.3Hz, 3H) Starting Material Synthesis Example 35 In addition, in the same manner as in Starting Material Synthesis Example 19 and using ethyl 6-bromo-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 6-bromo-2-(2-amino-5-fluoroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 36 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (6.0 g), 2,5-dichloronitrobenzene (5.8 g) and dimethyl sulfoxide (70 ml), crude crystals (9.5 g) of ethyl 2-(4-chloro-2-nitroaiiilmo)benzo[b]thiophene-3-carboxylate were obtained. Without purification, in the same manner as in Starting Material Synthesis Example 21 and using ethanol (100 ml), 18% hydrochloric acid (100 ml) and tin(II) chloride·dihydrate (22.5 g), ethyl 2-(2-amino-4-chloroanilino)benzo[b]thiophene-3-carboxylate (3.0 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 9.51(s, 1H), 8.11(d, J=8.3Hz, 1H), 7.46(d, J=7.8Hz, 1H), 7.29(dd, J=7.3, 8.3Hz, 1H), 7.23(d, J=8.3Hz, 1H), 7.11(dd, J=7.3, 3.8Hz, 1H), 6.81(s, 1H), 6.75(d, J=8.3Hz, 1H), 4.45(q, J=7.3Hz, 2H), 3.95(br.s, 2H), 1.48(t, J=7.3Hz, 3H) Starting Material Synthesis Example 37 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4-chloro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4-chloroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 38 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (6.0 g), 2,5-dibromonitrobenzene (8.5 g) and dimethyl sulfoxide (70 ml), crude crystals (7.3 g) of ethyl 2-(4-bromo-2-nitroanilino)benzo[b]thiophene-3-carboxylate were obtained. Without purification, in the same manner as in Starting Material Synthesis Example 21 and using ethanol (70 ml), 18% hydrochloric acid (70 ml) and tin(II) chloride•dihydrate (15.7 g), ethyl 2-(2-amino-4-bromoanilino)benzo[b]thiophene-3-carboxylate (3.3 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 9.52(s, 1H), 8.11(d, 1H), 7.46(d, J=8.3Hz, 1H), 7.31(dd, 1H), 7.18(d, J=8.3Hz, 1H), 7.11(dd, 1H), 6.99(s, 1H), 6.90(d, 1H), 4.44(q, J=7.3Hz, 2H), 3.94(br.s, 2H), 1.48(t, J=7.3Hz, 3H) Starting Material Synthesis Example 39 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4-bromo-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4-bromoanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 40 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (3 g), 3-fluoro-4-nitrotoluene (2.1 g) and dimethyl sulfoxide (25 ml), ethyl 2-(5-methyl-2-nitroanilino)benzo[b]thiophene-3-carboxylate (5.4 g) was obtained. Without purification, in the same manner as in Starting Material Synthesis Example 19 and using 10% palladium-carbon (1.5 g), ethyl 2-(2-amino-5-methylanilino)benzo[b]thiophene-3-carboxylate (1.6 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 9.61(s, 1H), 8.14(d, J=7.8Hz, 1H), 7.47(d, J=7.8Hz, 1H), 7.32(dd, J=7.8, 7.8Hz, 1H), 7.13(s, 1H), 7.10(dd, J=7.8, 7.8Hz, 1H), 6.95(d, J=7.8Hz, 1H), 6.76(d, J=7.8Hz, 1H), 4.46(q, J=7.3Hz, 2H), 3.73(br,s, 2H), 2.28(s, 3H), 1.50(t, J=7.3Hz, 3H) Starting Material Synthesis Example 41 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-amino-6-methylbenzo[b]thiophene-3-carboxylate (4.18 g), 2-fluoronitrobenzene (2.5 g) and dimethyl sulfoxide (50 ml), crude crystals (7.5 g) of ethyl 6-methyl-2-(2-nitroanilino)benzo[b]thiophene-3-carboxylate were obtained. Without purification, in the same manner as in Starting Material Synthesis Example 21 and using ethanol (50 ml), 18% hydrochloric acid (70 ml) and tin(II) chloride dihydrate (16 g), ethyl 2-(2-aminoanilino)-6-methylbenzo[b]thiophene-3-carboxylate (3.7 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 9.53(br.s, 1H), 7.99(d, J=8.3Hz, 1H), 7.32(d, J=7.8Hz, 1H), 7.25(s, 1H), 7.13(m, 2H), 6.83-6.80(m, 2H), 4.44(q, J=7.3Hz, 2H), 3.86(br.s, 2H), 2.37(s, 3H), 1.47(t, J=7.3Hz, 3H) Starting Material Synthesis Example 42 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 6-methyl-2-(2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-aminoanilino)-6-methylbenzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 43 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (6.0 g), 2,4,5-trichloronitrobenzene (6.8 g) and dimethyl sulfoxide (70 ml), crude crystals (10.3 g) of ethyl 2-(4,5-dichloro-2-nitroanilino)benzo[b]thiophene-3-carboxylate were obtained. Without purification, in the same manner as in Starting Material Synthesis Example 21 and using ethanol (100 ml), 18%hydrochloric acid (100 ml) and tin(II) chloride·dihydrate (22.8 g), ethyl 2-(2-amino-4,5-dichloroanilino)benzo[b]thiophene-3-carboxylate (5.3 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 9.33(s, 1H), 8.15(d, J=7.3Hz, 1H), 7.44(d, J=7.8Hz, 1H), 7.32(dd, J=7.8, 7.3Hz, 1H), 7.25(d, J=8.8Hz, 1H), 7.12(dd, J=7.8, 7.3Hz, 1H), 6.69(d, J=8.8Hz, 1H), 4.47(q, J=7.3Hz, 2H), 4.10(br.s, 2H), 1.49(t, J=7.3Hz, 3H) Starting Material Synthesis Example 44 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(4,5-dichloro-2-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4,5-dichloroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 45 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (6.6 g), 2,3,4-trichloronitrobenzene (7.0 g) and dimethyl sulfoxide (70 ml), crude crystals (12 g) o ethyl 2-(2,3-dichloro-6-nitroanilino)benzo[blthiophene-3-carboxylate were obtained. Without purification, in the same manner as in Starting Material Synthesis Example 21 and using ethanol (40 ml), 18% hydrochloric acid (60 ml) and tin(II) chloride·dihydrate (24.6 g), ethyl 2-(6-amino-2,3-dichloroanilino)benzo[b]thiophene-3-carboxylate (4.2 g) was obtained. Starting Material Synthesis Example 46 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(2,3-dichloro-6-nitroanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(6-amino-2,3-dichloroanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 47 In the same manner as in Starting Material Synthesis Example 4 and using ethyl 2-aminobenzo[b]thiophene-3-carboxylate (5.0 g), 4-fluoro-3-nitrobenzotrifluoride (5.1 g) and dimethyl sulfoxide (65 ml), ethyl 2-(2-nitro-4-trifluoromethylanilino)benzo[b]thiophene-3-carboxylate (12 g) was obtained. Without purification, in the same manner as in Starting Material Synthesis Example 21 and using ethanol (50 ml), 18% hydrochloric acid (50 ml) and tin(II) chloride·dihydrate (10.9 g), ethyl 2-(2-amino-4-trifluoromethylanilino)benzo[b]thiophene-3-carboxylate (2.8 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 9.93(br.s, 1H), 8.16(d, J=8.3Hz, 1H), 7.52(m, 2H), 7.36(dd, J=7.3, 8.3Hz, 1H), 7.17(dd, J=7.3, 6.9Hz, 1H), 7.08(m, 2H), 4.48(q, J=7.3Hz, 2H), 4.08(br.s, 2H), 1.51(t, J=7.3Hz, 3H) Starting Material Synthesis Example 48 In the same manner as in Starting Material Synthesis Example 19 and using ethyl 2-(2-amino-4-trifluoromethylanilino)benzo[b]thiophene-3-carboxylate, ethyl acetate, 10% palladium-carbon and hydrogen (60 atm kg/cm 2 ), ethyl 2-(2-amino-4-trifluoromethylanilino)benzo[b]thiophene-3-carboxylate is obtained. Starting Material Synthesis Example 49 Ethyl 2-(2-nitroanilino)benzo[b]thiophene-3-carboxylate (6.0 g) was dissolved in N,N-dimethylformamide (120 ml) and the mixture was stirred at 0°C, during which potassium tert-butoxide (3.0 g) was added portionwise. Methyl iodide (12.4 g) was added dropwise under ice-cooling and the mixture was stirred at 0°C for 5 min and heated to room temperature. The reaction mixture was extracted with ethyl acetate, washed with saturated brine and dried over anhydrous magnesium sulfate. After natural filtration, the solvent was evaporated under reduced pressure, and the residue was separated by silica gel column chromatography (hexane:ethyl acetate=4:1) to give crude crystals (2.8 g) of ethyl 2-(N-methyl-2-nitroanilino)benzo[b]thiophene-3-carboxylate. Without purification, the crystals were treated in the same manner as in Starting Material Synthesis Example 21 using ethanol (50 ml), 18% hydrochloric acid (50 ml) and tin(II) chloride·dihydrate (12.6 g) to give ethyl 2-(2-amino-N-methylanilino)benzo[b]thiophene-3-carboxylate (3.2 g). 1 H-NMR(400MHz, CDCl 3 ) δ: 8.07(d, 1H), 7.49(d, 1H), 7.30-7.24(m, 2H), 7.13-7.08(m, 2H), 6.79-6.74(m, 2H), 4.26(q, J=7.3Hz, 2H), 4.10(br.s, 2H), 3.36(s, 3H), 1.33(t, J=7.3Hz, 3H) Example 1 Ethyl 2-(2-aminoanilino)benzo[b]thiophene-3-carboxylate (3.5 g) and 1-methylpiperazine (20 ml) were dissolved in anisole (50 ml), titanium tetrachloride (3.1 ml) was added dropwise while stirring the mixture at room temperature. After the completion of dropwise addition, the mixture was stirred at 140°C for 20 hours. The reaction mixture was cooled to 80°C and poured into ice water. The resultant precipitate was filtrated. The filtrate was extracted with ethyl acetate (900 ml), washed with saturated brine, and dried over MgSO 4 . The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/methanol) and the obtained oily substance was dissolved in ethyl acetate (50 ml), to which was added a solution (10 ml) of maleic acid (580 mg) in ethyl acetate. The precipitated crystals were collected by filtration and recrystallized from ethyl acetate/methanol to give 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 maleate (1.14 g). melting point 248-249°C (decomposition) Example 2 Ethyl 2-(2-amino-5-fluoroanilino)benzo[b]thiophene-3-carboxylate (24.5 g) and 1-methylpiperazine (135 ml) were dissolved in anisole (400 ml), and titanium tetrachloride (21 ml) was added dropwise while stirring the mixture at room temperature. After the completion of the dropwise addition, the mixture was stirred at 140°C for 17 hours. The reaction mixture was cooled to 80°C and poured into ice water. The resultant precipitate was filtrated and the filtrate was extracted with ethyl acetate, washed with saturated brine, and dried over magnesium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/methanol) and the obtained oily substance (17 g) was dissolved in ethyl acetate (400 ml). Thereto was added a solution of maleic acid (5.9 g) in ethyl acetate (100 ml) and ethanol (20 ml). The precipitated crystals were collected by filtration and recrystallized from ethanol to give 8-fluoro- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine· 1 maleate (10.2 g). melting point 225-227°C (decomposition). 1 H-NMR (400MHz, DMSO-d 6 ) δ: 8.35(s, 1H), 7.82(d, J=7.8Hz, 1H), 7.74(br, 1H), 7.37(dd, J=8.3, 6.8Hz, 1H), 7.25(dd, J=8.3, 6.8Hz, 1H], 6.89(dd, 1H), 6.78(dd, 1H), 6.63(dd, 1H), 6.03(s, 2H) 2.5-4.5(br.s, 8H), 2.82(s, 3H). IR (KBr):3435, 3283, 3060, 2837, 2614, 1616, 1580, 1463, 1387, 1353, 1152, 966, 865 cm -1 . MS:m/e 366. Anal. Calcd. for C 20 H 19 FN 4 S•C 4 H 4 O 4 :C, 59.74; H, 4.80; N, 11.61%. Found:C, 59.71; H, 4.82; N, 11.46%. Example 3 Alternatively, using 2 equivalents of maleic acid, 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine dimaleate monohydrate was obtained. melting point 123-125°C (decomposition) Example 4 Alternatively, using fumaric acid instead of maleic acid, 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1/2 fumarate (494 mg) was obtained. melting point 193-195°C (decomposition) Example 5 Alternatively, using 2 equivalents of hydrochloric acid instead of maleic acid, 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 hydrochloride•3 hydrate was obtained. melting point 268-270°C (decomposition) Example 6 In the same manner as in Example 1 and using ethyl 2-(2-amino-5-methylanilino)benzo[b]thiophene-3-carboxylate (1.6 g), 1-methylpiperazine (8 ml), anisole (25 ml), titanium tetrachloride (1.3 ml) and maleic add (100 mg), 8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine· 3/2 maleate (166 mg) was obtained. melting point 198-201°C (decomposition) Example 7 In the same manner as in Example 1 and using ethyl 2-(2-amino-5-chloroanilino)benzo[b]thiophene-3-carboxylate (2.6 g), 1-methylpiperazine (15 ml), anisole (50 ml) and titanium tetrachloride (2.3 ml), 8-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (363 mg) was obtained. melting point 87-90°C (decomposition) Example 8 In the same manner as in Example 1 and using ethyl 2-(2-amino-5-bromoanilino)benzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 8-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 9 In the same manner as in Example 1 and using ethyl 2-(2-amino-4-fluoroanilino)benzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 9-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 10 In the same manner as in Example 1 and using ethyl 2-(2-amino-4-methylanilino)benzo[b]thiophene-3-carboxylate (3.7 g), 1-methylpiperazine (19 ml), anisole (60 ml) and titanium tetrachloride (3.0 ml), 9-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (1.18 g) was obtained. melting point 184-186°C: Anal. Calcd. for C 21 H 22 N 4 S:C, 69.58; H, 6.12; N, 15.46%. Found:C, 69.45; H, 6.20; N, 15.11%. Example 11 In the same manner as in Example 1 and using ethyl 2-(2-amino-4-chloroanilino)benzo[b]thiophene-3-carboxylate (3.0 g), 1-methylpiperazine (17 ml), anisole (75 ml), titanium tetrachloride (2.9 ml) and maleic acid, 9-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine·2 maleate· 1/2 hydrate (1.37 g) was obtained. melting point 105-107°C (decomposition): Anal. Calcd. for C 20 H 19 N 4 S•2C 4 H 4 O 4 ·1/2H 2 O: C, 53.89; H, 4.52; N, 8.98%. Found: C, 53.94; H, 4.45; N, 9.00%. Example 12 In the same manner as in Example 1 and using ethyl 2-(2-amino-4-bromoanilino)benzo[b]thiophene-3-carboxylate (3.3 g), 1-methylpiperazine (17 ml), anisole (75 ml), titanium tetrachloride (3.0 ml) and maleic acid, 9-bromo-12-(4-methylpiperazin-1-yl]-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 maleate was obtained. melting point 113-114°C (decomposition) Anal. Calcd. for C 20 H 19 BrN 4 S•2C 4 H 4 O 4 : C, 50. 99; H, 4.13; N, 8.50%. Found: C, 50.73; H, 4.15; N, 8.60%. Example 13 In the same manner as in Example 1 and using ethyl 2-(2-amino-4-methoxy-anilino)benzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 9-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 14 In the same manner as in Example 1 and using ethyl 2-(2-amino-4,5-difluoroanilino)benzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 8, 9-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 15 In the same manner as in Example 1 and using ethyl 2-(2-amino-3,5-difluoroanilino)benzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 8,10-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 16 In the same manner as in Example 1 and using ethyl 2-(2-aminoanilino)-6-methylbenzo[b]thiophene-3-carboxylate (3.7 g), 1-methylpiperazine (80 ml), anisole (16 ml) and titanium tetrachloride (3 ml), 3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (1.4 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 7.65(d, J=8.3Hz, 1H), 7.38(s, 1H), 7.12(d, J=8.3Hz, 1H), 7.03(t, J=7.8Hz, 1H), 6.97(dd, J=7.3, 7.8Hz, 1H), 6.86(dd, J=7.3, 5.9Hz, 1H), 6.64(d, J=7.8Hz, 1H), 5.10(br.s, 1H), 4.20-1.75(m, 8H), 2.38(s, 3H), 2.33(s, 3H). Example 17 In the same manner as in Example 1 and using ethyl 2-(2-amino-5-fluoroanilino)-6-methylbenzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole, titanium tetrachloride and hydrochloric acid, 8-fluoro-3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 hydrochloride•3/2 hydrate was obtained. melting point 273-275°C (decomposition) Example 18 In the same manner as in Example 1 and using methyl 2-(2-aminoanilino)-5-methylbenzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 2-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 19 In the same manner as in Example 1 and using methyl 2-(2-amino-5-fluoroanilino)-5-methylbenzo[b]thiophene-3-carboxylate (1.82 g), 1-methylpiperazine (9.7 ml), anisole (30 ml), titanium tetrachloride (1.51 ml) and maleic acid, 8-fluoro-2-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 maleic acid•1/2H 2 O (928 mg) was obtained. melting point: 199-201°C (decomposition). Anal. Calcd. for C 21 H 21 FN 4 S•C 4 H 4 O 4 •1/2H 2 O: C, 59.39; H, 5.18; N, 11.08%. Found: C, 59.49; H, 4.98; N, 11.04%. Example 20 In the same manner as in Example 1 and using ethyl 2-(2-aminoanilino)-6-methoxybenzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 21 In the same manner as in Example 1 and using ethyl 2-(2-amino-5-fluoroanilino)-6-methoxybenzo[b]thiophene-3-carboxylate, 1-methylpiperazine, anisole and titanium tetrachloride, 8-fluoro-3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 22 In the same manner as in Example 1 and using ethyl 2-(2-amino-4,5-dichloroanilino)benzo[b]thiophene-3-carboxylate (5.3 g), 1-methylpiperazine (27 ml), anisole (120 ml), titanium tetrachloride (4.8 ml) and fumaric acid, 8, 9-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine·1 fumarate (571 mg) was obtained. melting point 243-245°C (decomposition): Anal. Calcd. for C 20 H 18 Cl 2 N 4 S•C 4 H 4 O 4 : C, 54.04; H, 4.16; N, 10.50%. Found:C, 54.00; H, 4.10; N, 10.41%. Example 23 In the same manner as in Example 1 and using ethyl 2-(6-amino-2,3-dichloroanilino)benzo[b]thiophene-3-carboxylate (4.2 g), 1-methylpiperazine (22 ml), anisole (100 ml), titanium tetrachloride (3.9 ml) and fumaric acid, 7, 8-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 fumarate (660 mg) was obtained. melting point 235-236°C (decomposition): Anal. Calcd. for C 20 H 18 Cl 2 N 4 S•C 4 H 4 O 4 : C, 54.04; H, 4.16; N, 10.50%. Found: C, 53.69; H, 4.19; N, 10.33%. Example 24 In the same manner as in Example 1 and using ethyl 2-(2-amino-4-trifluoromethylanilino)benzo[b]thiophene-3-carboxylate (2.8 g), 1-methylpiperazine (15 ml), anisole (60 ml), titanium tetrachloride (2.5 ml) and maleic acid, 12-(4-methylpiperazin-1-yl)-9-trifluoromethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 maleate (363 mg) was obtained. melting point 128-129°C: Anal. Calcd. for C 21 H 19 F 3 N 4 S•2C 4 H 4 O 4 :C, 53.70; H, 4.20; N, 8.64%. Found: C, 53.56; H, 4.26; N, 8.56%. Example 25 In the same manner as in Example 1 and using ethyl 6-bromo-2-(2-amino-5-fluoroanilino)benzo[b]thiophene-3-carboxylate (3.8 g), 1-methylpiperazine (16 ml), anisole (85 ml) and titanium tetrachloride (3.0 ml), 3-bromo-8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (1.16 g) was obtained. melting point 219-221°C: Anal. Calcd. for C 20 H 18 BrFN 4 S: C, 53.94; H, 4.07; N, 12.58%. Found: C, 53.72; H, 3.88; N, 12.41%. Example 26 In the same manner as in Example 1 and using ethyl 2-(N-methyl-2-nitroanilino)benzo[b]thiophene-3-carboxylate (3.2 g), 1-methylpiperazine, anisole (80 ml) and titanium tetrachloride (3.5 ml), 6-methyl-12-(4-methylpiperazin-1-yl)[1]benzothieno[2,3-b][1,5]benzodiazepine (166 mg) was obtained. melting point 88-90°C Starting Material Synthesis Example 50 To a solution of 2-aminobenzo[b]thiophene-3-carbonitrile (116 g), dimethylformamide (370 ml) and 2,4-difluoronitrobenzene (73 ml) was added pulverized potassium carbonate (276 g), and the mixture was stirred at 55°C for 5.5 hours. The reaction mixture was stirred under ice-cooling for 30 minutes and water (1.9 L) was added. The mixture was further stirred for 30 minutes. The precipitated crystals were filtered by suction and washed with water (1.9 L) and dried in a drying box overnight to give crude crystals (198 g). The obtained crude crystals were suspended in ethyl acetate (700 ml) and the mixture was stirred under reflux with heating while removing water for 2 hours. The reaction mixture was cooled and stirred at 20°C for 1 hour. The precipitated crystals were filtered by suction, washed with ethyl acetate (150 ml) and dried to give 2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (123 g) as red crystalline powder. melting point 161-163°C Starting Material Synthesis Example 51 To a solution of 2-amino-6-fluorobenzo[b]thiophene-3-carbonitrile (15 g), tetrahydrofuran (140 ml) and 2-fluoronitrobenzene (11.3 g) was added portionwise 60% sodium hydride (3.5 g) under ice-cooling and the mixture was stood still at 5°C for 30 minutes. The precipitated crystals were filtered by suction, washed with isopropyl ether (120 ml) and dried to give 6-fluoro-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile (8.0 g). 1 H-NMR(400MHz, CDCl 3 ) δ: 10.13(br.s, 1H), 8.28(d, J=8.3Hz, 1H), 7.75(m, 1H), 7.61-7.57(m, 2H), 7.43(m, 1H), 7.25(m, 1H), 7.10(m, 1H) Starting Material Synthesis Example 52 In the same manner as in Starting Material Synthesis Example 51 and using 2-amino-6-chlorobenzo[b]thiophene-3-carbonitrile and 2-fluoronitrobenzene, 6-chloro-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile is obtained. Starting Material Synthesis Example 53 In the same manner as in Starting Material Synthesis Example 51 and using 2-amino-6-methoxybenzo[b]thiophene-3-carbonitrile (5.2 g) and 2,4-difluoro-nitrobenzene (4.0 g), 6-methoxy-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (2.5 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 10.04(br.s, 1H), 8.33(m, 1H), 7.74(d, J=8.8Hz, 1H), 7.23(s, 1H), 7.13(d, J=8.8Hz, 1H), 7.04(d, J=10.3Hz, 1H), 6.70(m, 1H), 3.88(s, 3H) Starting Material Synthesis Example 54 In the same manner as in Starting Material Synthesis Example 51 and using 2-amino-4-methylbenzo[b]thiophene-3-carbonitrile (3.8 g), 2-chloronitrobenzene(3.5 g) and dimethyl sulfoxide (45 ml), 4-methyl-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile (5.8 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 10.31 (br.s, 1H), 8.28(d, J=8.3Hz, 1H), 7.66-7.52(m, 3H), 7.27-7.11(m, 2H), 7.08(d, J=7.4Hz, 1H), 2.81(s, 3H) Starting Material Synthesis Example 55 In the same manner as in Starting Material Synthesis Example 51 and using 2-amino-7-methylbenzo[b]thiophene-3-carbonitrile and 2-fluoronitrobenzene, 7-methyl-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile is obtained. Starting Material Synthesis Example 56 In the same manner as in Starting Material Synthesis Example 51 and using 2-amino-6-fluorobenzo[b]thiophene-3-carbonitrile (4.9 g) and 2,4-difluoronitrobenzene (4.1 g), 6-fluoro-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (7.8 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 10.23(br.s, 1H), 8.35(dd, J=9.3, 5.1Hz, 1H), 7.81(dd, J=8.8, 4.9Hz, 1H), 7.48(d, J=8.3Hz, 1H), 7.31-7.28(m, 1H), 7.14(d, J=9.3Hz, 1H), 6.76(m, 1H) Starting Material Synthesis Example 57 In the same manner as in Starting Material Synthesis Example 51 and using 2-amino-6-fluorobenzo[b]thiophene-3-carbonitrile (5.0 g), 3-fluoro-4-nitrotoluene (4.7 g) and dimethyl sulfoxide (55 ml), 6-fluoro-2-(5-methyl-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (7.5 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 10.19(br.s, 1H), 8.17(d, J=8.8Hz, 1H), 7.75(m, 1H), 7.44(d, J=8.3Hz, 1H), 7.24-7.22(m, 2H), 6.89(d, J=8.8Hz, 1H), 2.41(s, 3H) Starting Material Synthesis Example 58 In the same manner as in Starting Material Synthesis Example 51 and using 2-aminobenzo[b]thiophene-3-carbonitrile (4.0 g) and 2,5-difluoronitrobenzene (3.7 g), 2-(4-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (6.7 g) was obtained. 1 H-NMR (400MHz, CDCl 3 ) δ: 9.94(br.s, 1H), 8.01(m, 1H), 7.79(d, J=7.9Hz, 1H), 7.71(d, J=8.3Hz, 1H), 7.62(m, 1H), 7.50(dd, J=7.3, 8.3Hz, 1H), 7.42-7.35(m, 2H) Starting Material Synthesis Example 59 Sodium hydrosulfite (86%, 233 g) was suspended in dimethylformamide (240 ml) and the suspension was heated to 110°C. To this suspension was added dropwise at 110°C a solution of 2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (120 g), dimethylformamide (720 ml) and water (27.5 ml), which solution had been heated to 65-70°C. The reaction mixture was stirred atl 10°C for 1 hour and cooled to 20°C. Water (3.3 L) was added in 6 portions over 1 hour and the mixture was stirred at 20°C for 1.5 hours. The precipitated crystals were filtered by suction and washed with water (2 L) to give 2-(2-amino-5-fluoroanilino)benzo[b]thiophene-3-carbonitrile (158 g, (wet weight)) as ocher crystals. melting point 178-180°C (decomposition) Example 27 2-(2-Amino-5-fluoroanilino)benzo[b]thiophene-3-carbonitrile (155 g) was suspended in a mixture of methanol (250 ml) and water (200 ml) and 35% aqueous hydrochloric acid (42 ml) was added. The mixture was stirred under reflux with heating for 5.5 hours. The reaction mixture was cooled and stirred at 20°C for 1 hour. The precipitated crystals were filtered by suction, washed successsively with water (500 ml) and isopropyl alcohol (200 ml) and dried in a drying box overnight to give 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (104 g) as yellow crystals. melting point 213-216°C (decomposition) Example 28 Tin(II) chloride (50 g) was dissolved in a 18% hydrochloric acid (250 ml) solution heated to 50°C, and a suspension of 6-fluoro-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile (20 g) in ethanol (280 ml) was added. The mixture was refluxed under heating for 4 hours. Then, the reaction mixture was cooled to 40°C and poured into water (1 L) and the mixture was stood still for 30 minutes. The precipitated crystals were filtered by suction and washed with water (500 ml) to give 12-amino-3-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (15 g). 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.46(s, 1H), 9.92(s, 1H), 9.02(s, 1H), 9.00(s, 1H), 7.84(d, J=6.3Hz, 1H), 7.69(m, 1H), 7.31(m, 1H), 7.20-6.98(m, 4H) Example 29 In the same manner as in Example 28 and using 6-chloro-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile, 12-amino-3-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride is obtained. Example 30 In the same manner as in Example 28 and using 6-methoxy-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (2.5 g), 12-amino-8-fluoro-3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (2.1 g) was obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.68(br.s, 1H), 10.02(br.s, 1H), 9.09(br.s, 1H), 9.01(br.s, 1H), 7.58(d, J=8.8Hz, 1H), 7.53(s, 1H), 7.10-6.95(m, 3H), 6.86(d, J=9.3Hz, 1H), 3.78(s, 3H) Example 31 In the same manner as in Example 28 and using 4-methyl-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile (2.5 g), 12-amino-1-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride was obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.32(s, 1H), 10.02(s, 1H), 9.08(s, 1H), 8.64(s, 1H), 7.71(d, J=7.3Hz, 1H), 7.27-7.15(m, 5H), 7.02(d, 7.3Hz, 1H), 2.36(s, 3H) Example 32 In the same manner as in Example 28 and using 7-methyl-2-(2-nitroanilino)benzo[b]thiophene-3-carbonitrile, 12-amino-4-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride is obtained. Example 33 In the same manner as in Example 28 and using 6-fluoro-2-(5-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (7.8 g), 12-amino-3,8-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (4.8 g) was obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.51(s, 1H), 10.08(s, 1H), 9.05(s, 2H), 7.86(d, J=8.7Hz, 1H), 7.70(m, 1H), 7.32(m, 1H), 7.11(m, 1H), 7.00(m, 1H), 6.86(d, J=9.3Hz, 1H) Example 34 In the same manner as in Example 28 and using 6-fluoro-2-(5-methyl-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (7.4 g), 12-amino-3-fluoro-8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (8.5 g) is obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.53(br.s, 1H), 9.92(br.s, 1H), 9.02(br.s, 1H), 8.95(br.s, 1H), 7.84(d, J=8.8Hz, 1H), 7.68(d, J=8.7Hz, 1H), 7.30(dd, J=8.8, 9.3Hz, 1H), 6.96-6.91(m, 2H), 6.79(s, 1H), 2.22(s, 3H) Example 35 In the same manner as in Example 28 and using 2-(4-fluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile (6.7 g), 12-amino-9-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (4.8 g) was obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.73(s, 1H), 10.06(s, 1H), 9.14(s, 1H), 9.11(s, 1H), 7.87(d, J=7.8Hz, 1H), 7.68(d, J=7.8Hz, 1H), 7.42(dd, J=8.3, 7.3Hz, 1H), 7.28(dd, J=7.8, 7.3Hz, 1H), 7.10-7.04(m, 2H), 6.93(dd, J=9.3, 2.4Hz, 1H) Example 36 In the same manner as in Starting Material Synthesis Example 51 and using 2-aminobenzo[b]thiophene-3-carbonitrile (5.0 g) and 2,4,5-trifluoronitrobenzene (5.1 g), crude crystals (8.7 g) of 2-(4,5-difluoro-2-nitroanilino)benzo[b]thiophene-3-carbonitrile were obtained. Without purification, in the same manner as in Example 28 and using tin(II) chloride• dihydrate (95 g), 18% hydrochloric acid (65 ml) and ethanol (120 ml), 12-amino-8, 9-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (7.7 g) was obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.36(br.s, 1H), 9.97(s, 1H), 9.16(br.s, 1H), 9.09(br.s, 1H), 7.90(d, J=7.8Hz, 1H), 7.69(d, J=8.3Hz, 1H), 7.44(t, J=7.8Hz, 1H), 7.32(dd, J=7.8, 7.3Hz, 1H), 7.21(dd, J=7.9, 8.7Hz, 1H), 7.05(t, J=7.8Hz, 1H) Example 37 To 12-(4-methylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (63.5 g) was added ethanol (320 ml) and the mixture was heated for dissolution. Active charcoal (1.9 g) was added for natural filtration and the mixture was washed with ethanol (220 ml). The solution was heated to 35°C and a solution of maleic acid (20.1 g) in an aqueous organo solution (95 ml, upon natural filtration after dissolution) was added dropwise with stirring. The solution was stirred at 35°C for 1 hour, cooled and stirred at 20°C for 2 hours. The precipitated crystals were collected by filtration, washed with ethanol (130 ml) and dried at 50°C to give 12-(4-methylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 maleate (50.5 g) as pale-yellow crystals. melting point 225-227°C (decomposition) Using a suitable organic acid or inorganic acid instead of maleic acid used in the above-mentioned Examples, the following salt compounds can be synthesized. Example 38 12-(4-methylpiperazin- 1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 methanesulfonate. melting point 303.5°C (decomposition). 1 H-NMR(400MHz, DMSO-d 6 ) δ: 9.64(br.s, 1H), 8.36(s, 1H), 7.82(d, 1H), 7.38(dd, 1H), 7.25(dd, 1H), 6.89(dd, 1H), 6.79(dd, 1H), 6.62(d, 1H), 3.10-3.60(m, 8H), 2.85(s, 3H), 2.30(s, 3H). IR (KBr): 3246, 1618, 1518, 1385 cm -1 . MS: m/e 366. Anal. Calcd. for C 20 H 19 FN 4 S•CH 4 O 3 S: C, 54.53; H, 5.01; N, 12.11%. Found:C, 54.35; H, 5.04; N, 11.94%. Example 39 12-(4-methylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 nitrate melting point 170°C (decomposition). 1 H-NMR (400MHz, DMSO-d 6 ) δ: 9. 78(br.s, 1H), 7.92(d, 1H), 7.58-7.77(m, 1H), 7.45(dd, 1H), 7.33(dd, 1H), 6.77-6.98(m, 3H), 3.20-3.90(m, 8H), 2.84(s, 3H). IR (KBr): 3462, 3043, 1599, 1508 cm -1 . MS:m/e 366. Anal. Calcd. for C 20 H 19 FN 4 S•2HNO 3 •0.5H 2 O: C, 47.90; H, 4.42; N, 16.76%. Found: C, 47.89; H, 4.44; N, 16.75%. Example 40 12-(4-methylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1.5 sulfate melting point 236-239°C. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 9.69(br, 1H), 7.87(d, 1H), 7.80-7.86(m, 1H), 7.42(dd, 1H), 7.30(dd, 1H), 6.60-7.10(m, 3H), 3.80-4.40(m, 8H). 2.89(s, 3H). IR (KBr): 3406, 2986, 1597, 1510 cm -1 . MS:m/e 366. Anal. Calcd. for C 20 H 19 FN 4 S•1.5H 2 SO 4 •1.5H 2 O:C, 44.43;H, 4.66;N, 10.36%. Found:C, 44.45;H, 4.65;N, 10.31% Example 41 12-(4-methylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 phosphate melting point 189-192°C. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 8.29(s, 1H), 7.81(d, 1H), 7.71(d, 1H), 7.35(dd, 1H), 7.23(dd, 1H), 6.87(dd, 3H), 6.70(d, 1H) 6.56(d, 1H), 2.40-3.90(m, 8H), 2.44(s, 3H). IR(KBr):3429, 2922, 1597, 1510 cm -1 . MS:m/e 366. Anal. Calcd. for C 20 H 19 FN 4 S•2H 3 PO 4 •1H 2 O: C, 41.38; H, 4.69; N, 9.65%. Found: C, 41.54; H, 5.11; N, 9.66%. Example 42 12-Amino-3-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine·1 hydrochloride (12 g) was dissolved in N-methylpiperazine (140 ml) and the mixture was stirred under reflux with heating for 3 hours. The reaction mixture is cooled to 40°C, extracted with ethyl acetate (250 ml) and washed with saturated brine (100 ml). The ethyl acetate layer is dried over magnesium sulfate. The solution was naturally filtrated and the solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate:methanol) to give crude crystals of 3-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine. The obtained crude crystals were dissolved in ethyl acetate (30 ml) and a solution of maleic acid (1.83 g) in ethanol (20 ml) was added. The precipitated crystals were collected by filtration and recrystallized from aqueous ethanol to give 3-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 maleate (2.82 g). melting point 209°C. Anal. Calcd. for C 20 H 19 FN 4 S•C 4 H 4 O 4 : C, 59.74; H, 4.80; N, 11.61%. Found: C, 59.66; H, 4.69; N, 11.56%. Example 43 In the same manner as in Example 42 and using 12-amino-3-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride and N-methylpiperazine, 3-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 44 In the same manner as in Example 42 and using 12-amino-8-fluoro-3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.6 g), N-methylpiperazine (50 ml) and citric acid, crude crystals (1.0 g) of 8-fluoro-3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine was obtained. The crystals were dissolved in ethanol (10 ml) and a solution of citric acid (552 mg) in ethanol (10 ml) was added. The precipitated salt was recrystallized from a a mixed solvent of ethyl acetate and ethanol to give 8-fluoro-3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 citrate•dihydrate (204 mg). melting point 118-120°C. Anal. Calcd. for C 21 H 21 FN 4 OS•C 6 H 8 O 7 •2H 2 O: C, 51.92; H, 5.33; N, 8.97%. Found: C, 52.18; H, 5.19; N, 8.63%. Example 45 In the same manner as in Example 42 and using 12-amino-1-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride and N-methylpiperazine, 1-methyl-12-(4-methylpiperazin-1-yl]-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 46 In the same manner as in Example 42 and using 12-amino-4-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride and N-methylpiperazine, 4-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 47 In the same manner as in Example 42 and using 12-amino-3, 8-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (8.0 g), N-methylpiperazine (100 ml) and maleic acid, 3, 8-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 maleate (3.4 g) was obtained. melting point 211-213°C. Anal. Calcd. for C 20 H 18 FN 4 S•C 4 H 4 O 4 : C, 57.59; H, 4.43; N, 11.19%. Found: C, 57.58; H, 4.37; N, 11.10%. Example 48 In the same manner as in Example 42 and using 12-amino-3-fluoro-8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (8.5 g), N-methylpiperazine (100 ml) and hydrochloric acid, 3-fluoro-8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 hydrochloride was obtained. melting point 240-242°C (decomposition). 1 H-NMR(400MHz, DMSO-d 6 ) δ: 11.82(br.s, 2H), 11.09(br.s, 1H), 7.89-7.54(m, 2H), 7.33-7.26(m, 2H), 6.98-6.96(m, 2H), 4.79-2.87(m, 11H), 2.24(s, 3H) Example 49 In the same manner as in Example 42 and using 12-amino-9-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (2.0 g), N-methylpiperazine (40 ml) and maleic acid, 9-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 maleate (1.2 g) was obtained. melting point 136-137°C. Anal. Calcd. for C 20 H 19 FN 4 S•2C 4 H 4 O 4 : C, 56.18; H, 4.55; N, 9.36%. Found: C, 56.14; H, 4.58; N, 9.32%. Example 50 In the same manner as in Example 42 and using 12-amino-8, 9-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (4.0 g), N-methylpiperazine (40 ml) and maleic acid, 8, 9-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 maleate (924 mg) was obtained. melting point 144-146°C (decomposition). Anal. Calcd. for C 20 H 18 F 2 N 4 S•C 4 H 4 O 4 :C, 57.59; H, 4.43; N, 11.19%. Found:C, 57.39;H, 4.44;N, 11.10%. Example 51 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.3 g), N-ethylpiperazine (25 ml) and maleic acid, 12-(4-ethylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 maleate (731 mg) was obtained. melting point 211-212°C. Anal. Calcd. for C 21 H 21 FN 4 S•C 4 H 4 O 4 :C, 60.47;H, 5.07;N, 11.28%. Found:C, 60.30;H, 5.03;N, 11.18%. Example 52 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.5 g), N-n-propylpiperazine (17 g), dimethyl sulfoxide (10 ml) and hydrochloric acid, crude crystals (850 mg) of 8-fluoro-12-(4-propylpiperazin-1-yl)-5H-[1]benzothieno[2,3-b][1,5]benzodiazepine were obtained. The crude crystals were dissolved in ethyl acetate (12 ml) and a solution of 5.5 mol/l hydrochloric acid in ether was added to give 8-fluoro- 12-(4-propylpiperazin-1-yl]-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 hydrochloride•1.5 hydrate (757 mg). melting point 215-217°C (decomposition). Anal. Calcd. for C 22 H 23 FN 4 S·2HCl•3/2H 2 O:C, 53.44;H, 5.71;N, 11.33%. Found:C, 53.33;H, 5.78;N, 11.14%. Example 53 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.5 g), N-isopropylpiperazine (17 g), dimethyl sulfoxide (10 ml) and maleic acid, 8-fluoro-12-(4-isopropylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine·1 maleate (521 mg) was obtained. melting point 217°C. Anal. Calcd. for C 22 H 23 FN 4 S•C 4 H 4 O 4 :C, 61.16;H, 5.33;N, 10.97%. Found:C, 61.03;H, 5.30;N, 10.91%. Example 54 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.7 g), N-cyclopropylpiperazine (8.0 g), dimethyl sulfoxide (10 ml) and maleic acid, 12-(4-cyclopropylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine-1 maleate (877 mg) was obtained. melting point 182-183°C. Anal. Calcd. for C 22 H 21 FN 4 S•C 4 H 4 O 4 :C, 61.40;H, 4.95;N, 11.02%. Found:C, 61.28;H, 4.88;N, 10.82%. Example 55 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.3 g), N-(2-hydroxyethyl)piperazine (12.5 ml), dimethyl sulfoxide (5 ml) and maleic acid, 8-fluoro-12-[4-(2-hydroxyethyl)piperazin-1-yl]-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 maleate·1 hydrate (1.18 g) was obtained. melting point 75-77°C. Anal. Calcd. for C 21 H 21 FN 4 OS•2C 4 H 4 O 4 •H 2 O: C, 53.86;H, 4.83;N, 8.66%. Found:C, 53.80;H, 4.94;N, 8.67%. Example 56 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.4 g), N-methylhomopiperazine (5.4 g), dimethyl sulfoxide (25 ml) and citric acid, 8-fluoro-12-(4-methylhomopiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine·1 citrate·0.5 hydrate (455 mg) was obtained. melting point 98-100°C (decomposition). Anal. Calcd. for C 21 H 21 FN 4 S•C 6 H 8 O 7 •1/2H 2 O:C, 55.76; H, 5.20; N, 9.63%. Found:C, 55.48;H, 5.60;N, 9.64%. Example 57 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.3 g), N, N, N'-trimethylethylenediamine (13 g), dimethyl sulfoxide (5 ml) and citric acid, 8-fluoro-12-[N-(2-dimethylaminoethyl)-N-methylamino]-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine-1 citrate•1 hydrate (289 mg) was obtained. melting point 72-74°C (decomposition). Anal. Calcd. for C 20 H 21 FN 4 S•C 6 H 8 O 7 •H 2 O:C, 53.97;H, 5.40;N, 9.68%. Found:C, 53.60;H, 5.27;N, 9.02%. Example 58 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (1.6 g), 1-[2-(2-hydroxyethoxy)ethyl]piperazine (13 g) and dimethyl sulfoxide (5 ml), 8-fluoro-12-{4-[2-(2-hydroxyethoxy)ethyl]-1-piperazinyl}-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (422 mg) was obtained. melting point 198°C. Anal. Calcd. for C 23 H 25 FN 4 O 2 S:C, 62.71; H, 5.72;N, 12.72%. Found:C, 62.63;H, 5.59;N, 12.57%. Example 59 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (2.1 g), N-benzylpiperazine (25 g), dimethyl sulfoxide (10 ml) and hydrochloric acid, 8-fluoro- 12-(4-benzylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•2 hydrochloride•1/4 hydrate (360 mg) was obtained. melting point 260-262°C. Example 60 In the same manner as in Example 42 and using 12-amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride (2.0 g), tert-butyl-1-piperazinecarboxylate (5.0 g) and dimethyl sulfoxide (15 ml), 8-fluoro-12-(4-tert-butoxycarbonylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (1.6 g) was obtained. 1 H-NMR(400MHz, CDCl 3 ) δ: 7.76(d, J=7.8Hz, 1H), 7.60(d, J=7.8Hz, 1H), 7.32(dd, J=7.4, 7.1Hz, 1H), 7.22(dd, J=9.2, 8.3Hz, 1H), 6.97(dd, J=8.8, 2.9Hz, 1H), 6.70(dd, J=8.3, 2.8Hz, 1H), 6.42(dd, J=8.8, 2.9Hz, 1H), 5.18(br.s, 1H), 4.20-0.70(m, 9H), 1.44(s, 9H). Example 61 8-Fluoro-12-(4-tert-butoxycarbonylpiperazin- 1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (1.6 g) was dissolved in trifluoroacetic acid (8 ml) and the mixture was stirred at room temperature for 3 hours. Trifluoroacetic acid was evaporated under reduced pressure. The residue was dissolved in ethyl acetate and saturated aqueous sodium hydrogencarbonate solution was added. The mixture was stirred at room temperature for 2 hours and the ethyl acetate layer was dried over magnesium sulfate. After natural filtration, the solvent was evaporated and the residue was separated and purified by silica gel column chromatography (chloroform:methanol=4:1) to give 8-fluoro-12-(piperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (1.7 g), which was treated with maleic acid to give 8-fluoro-12-(piperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•3/2 maleate•1/2 hydrate (347 mg). melting point 141-143°C (decomposition). Anal. Calcd. for C 19 H 17 FN 4 S•3/2C 4 H 4 O 4 •1/2H 2 O: C, 56.17; H, 4.34; N, 10.48%. Found:C, 56.30;H, 4.58;N, 10.08%. Example 62 8-Fluoro-3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (500 mg) was dissolved in dichloromethane (15 ml) and ethanedithiol (2.10 ml) and aluminium chloride (2.51 g) were added. The mixture was stirred at room temperature for 2 hours. Water (50 ml) was added to the reaction mixture and 10% aqueous sodium hydroxide solution was added until it assumed alkaline. The mixture was extracted with chloroform, washed with water, washed with saturated aqeuous sodium chloride solution and dried over sodium sulfate. The solvent was evaporated under reduced pressure, and the residue was separated by silica gel column chromatography (chloroform:methanol=5:1) and recrystallized from ethyl acetate to give 8-fluoro-3-hydroxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (251 mg). 1 H-NMR(400MHz, CDCl 3 ) δ: 7.59(d, 1H), 7.01(d, 1H), 6.96(dd, 1H), 6.83(dd, 1H), 6.68(m, 1H), 6.41(dd, 1H), 5.09(br.s, 1H), 4.00-2.00(m, 8H), 2.34(s, 3H). Example 63 8-Fluoro-12-(piperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (260 mg) was dissolved in toluene (5 ml) and anhydrous acetic acid (230 mg) and triethylamine (375 mg) were added. The mixture was stirred at 80°C for 30 minutes. The reaction mixture was allowed to cool to room temperature, and extracted with chloroform, washed with water and dried over anhydrous magnesium sulfate. After natural filtration, the solvent was evaporated under reduced pressure and the residue was recrystallized from methanol to give 8-fluoro-12-(4-acetylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine· 1/4 hydrate (97 mg). melting point >290°C. Anal. Calcd. for C 21 H 19 FN 4 OS•1/4H 2 O: C, 63.22; H, 4.93; N, 14.04%. Found:C, 62.97;H, 4.67;N, 13.75%. Example 64 To a solution of 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (100 mg) in ethanol (2 ml) was added 30% aqueous hydrogen peroxide solution (0.1 ml) and the mixture was stirred at room temperature for 3 hours and then left standing for one day. The obtained suspension was filtered and the solvent was evaporated under reduced pressure. The obtained residue was purified by silica gel column chromatography (chloroform:methanol=5:1) to give 8-fluoro-12-(4-methylpiperazine-4-oxide-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (50 mg) as a yellow oily substance. 1 H-NMR(400MHz, CDCl 3 ) δ: 3.00-3.80(8H, m, CH 2 ×4), 3.25(3H, s, CH 3 ), 6.46(1H, dd, J=2.9, 8.8Hz, ArH), 6.74(1H, ddd, J=2.4, 2.9, 8.3Hz, ArH), 6.97(1H, dd, J=2.9, 8.8Hz, ArH), 7.24(1H, d, J=7.4Hz, ArH), 7.37(1H, dd, J=7.3, 7.8Hz, ArH), 7.63(1H, d, J=8.3Hz, ArH), 7.72(1H, d, J=8.3Hz, ArH). MS: m/e 383(M+1). Example 65 In the same manner as in Example 62 and using 8-fluoro-1-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, dichloromethane, ethanedithiol and aluminium chloride, 8-fluoro-1-hydroxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 66 In the same manner as in Example 62 and using 8-fluoro-2-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, dichloromethane, ethanedithiol and aluminium chloride, 8-fluoro-2-hydroxy 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 67 In the same manner as in Example 62 and using 8-fluoro-4-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, dichloromethane, ethanedithiol and aluminium chloride, 8-fluoro-4-hydroxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 68 In the same manner as in Example 62 and using 8-fluoro-7-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, dichloromethane, ethanedithiol and aluminium chloride, 8-fluoro-7-hydroxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 69 In the same manner as in Example 62 and using 8-fluoro-9-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, dichloromethane, ethanedithiol and aluminium chloride, 8-fluoro-9-hydroxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 70 In the same manner as in Example 62 and using 8-fluoro-10-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, dichloromethane, ethanedithiol and aluminium chloride, 8-fluoro-10-hydroxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is obtained. Example 71 3-Fluoro-8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine is dissolved in carbon tetrachloride and reacted with 2, 2'-azobis(isobutylonitrile) in the presence of N-chlorosuccinimide to give 8-chloromethyl-3-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine. Example 72 12-Amino-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine hydrochloride (102 g) was suspended in toluene (600 ml), and the mixture was stirred under reflux with heating for 3 hours to remove water. The mixture was cooled to 70°C under a nitrogen atmosphere, and N-methylpiperazine (300 ml) was added. The mixture was stirred with heating under a nitrogen atmosphere until the inside temperature reached 120°C while removing toluene for 1.5 hours. The mixture was heated under a nitrogen atmosphere at 120°C for 2.5 hours and cooled to 25°C under a nitrogen atmosphere. Ethyl acetate (600 ml) and water (450 ml) were added to the reaction mixture for extraction and the obtained ethyl acetate layer was washed three times with water (300 ml). After confirming pH 7-8 of washing water, the solvent was concentrated under reduced pressure. Acetonitrile (100 ml) was added and the mixture was azeotropically concentrated to dryness under reduced pressure. To the residue was added acetonitrile (300 ml) and the mixture was heated for dissolution. The solution was cooled and stirred at -10°C - -5°C for 2.5 hours to allow precipitation of crystals. The precipitated crystals were filtered by suction and washed with acetonitrile (100 ml), followed by drying to give 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine (65.5 g). melting point167-169°C. Example 73 Using an equivalent amount of hydrochloric acid instead of maleic acid in Example 2, 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine•1 hydrochloride was obtained. melting point 310.3°C. MS:m/e 366, Anal. Calcd. for C 20 H 19 FN 4 S•HCl:C, 59.62; H, 5.00;N, 13.91%. Found:C, 59.43;H, 5.02;N, 13.88%. Starting Material Synthesis Example 60 2-Bromo-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid (6 g) was suspended in thionyl chloride (30 ml) and the mixture was stirred under reflux with heating for 75 minutes. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (50 ml). This toluene solution was added dropwise to a solution of 2-aminophenol (2.5 g) in pyridine (50 ml) under ice-cooling. The mixture was stirred at room temperature for 2 hours and completion of the reaction was confirmed by thin layer chromatography. The solvent was evaporated under reduced pressure and the residue was dissolved in ethyl acetate. The mixture was washed with diluted hydrochloric acid and saturated aqueous sodium hydrogencarbonate solution and dried over magnesium sulfate. The solvent was evaporated under reduced pressure. To the residue was added diisopropyl ether and the mixture was filtrated to give 2-bromo-4,5,6,7-tetrahydro-N-(2-hydroxyphenyl)-benzo[b]thiophene-3-carboxamide (5.3 g). 1 H-NMR(400MHz, CDCl 3 ) δ: 8.65(br, 1H), 7.94(br, 1H), 7.15-7.19(m, 2H), 7.06(d, J=8.3Hz, 1H), 6.91(m, 1H), 2.75-2.78(m, 2H), 2.67-2.70(m, 2H), 1.77-1.86(m, 4H) Starting Material Synthesis Example 61 In the same manner as in Starting Material Synthesis Example 60 and using 2-bromo-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid (6 g), thionyl chloride (30 ml), 2-amino-5-methylphenol (3.14 g), 2-bromo-4,5,6,7-tetrahydro-N-(2-hydroxy-4-methylphenyl)-benzo[b]thiophene-3-carboxamide (5.8 g) was obtained. 1 H-NMR(400MHz, DMSO-d 6 ) δ: 9.66(s, 1H), 9.33(br, 1H), 7.62(d, J=8.3Hz, 1H), 6.71(s, 1H), 6.62(d, J=8.3Hz, 1H), 2.66-2.50(m, 4H), 2.22(s, 3H), 1.76-1.72(m, 4H) Starting Material Synthesis Example 62 In the same manner as in Starting Material Synthesis Example 60 and using 2-bromo-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid, thionyl chloride and 2-amino-5-fluorophenol, 2-bromo-4,5,6,7-tetrahydro-N-(4-fluoro-2-hydroxyphenyl)-benzo[b]thiophene-3-carboxamide is obtained. Starting Material Synthesis Example 63 In the same manner as in Starting Material Synthesis Example 60 and using 2-bromo-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid, thionyl chloride, 2-aminothiophenol, 2-bromo-4,5,6,7-tetrahydro-N-(2-mercaptophenyl)-benzo[b]thiophene-3-carboxamide is obtained. Starting Material Synthesis Example 64 In the same manner as in Starting Material Synthesis Example 60 and using 2-bromo-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid, thionyl chloride and 2-amino-5-methylthiophenol, 2-bromo-4,5,6,7-tetrahydro-N-(2-mercapto-4-methylphenyl)-benzo[b]thiophene-3-carboxamide is obtained. Starting Material Synthesis Example 65 In the same manner as in Starting Material Synthesis Example 60 and using 2-bromo-4, 5, 6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid, thionyl chloride, 2-amino-5-fluorothiophenol, 2-bromo-4,5,6,7-tetrahydro-N-(4-fluoro-2- mercaptophenyl)-benzo[b]thiophene-3-carboxamide is obtained. Starting Material Synthesis Example 66 2-Aminobenzenethiol (1.1 g) was dissolved in N,N-dimethylformamide (25 ml) and 60% sodium hydride (360 mg) was added. The mixture was stirred at room temperature for 20 minutes. To the mixture was added ethyl 2-bromo-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylate (2.6 g), and the mixture was stirred at 140°C for 1.5 hours and allowed to cool to room temperature. The reaction mixture was extracted with ethyl acetate (300 ml), washed with water (200 ml) and dried over anhydrous sodium sulfate. The solvent was evaporated under reduced pressure and the obtained crude crystals were washed with isopropyl ether and filtrated to give ethyl 2-(2-aminobenzenethio)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylate (1.4 g). 1 H-NMR(400MHz, CDCl 3 ) δ: 7.50(d, J=7.4Hz, 1H), 7.28(m, 1H), 6.80(m, 2H), 4.43(br.s, 2H), 4.38(q, J=7.3Hz, 2H), 2.78(m, 2H), 2.52(m, 2H), 1.73-1.60(m, 4H), 1.41(t, 3H) Starting Material Synthesis Example 67 2-Bromo-5-methyl-3-thiophenecarboxylic acid (5 g) was suspended in thionyl chloride (20 ml) and the mixture was stirred under reflux with heating for 75 minutes. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (5 ml). This toluene solution was added dropwise to a solution of 2-aminophenol (3.7 g) in pyridine (30 ml) at 0°C. The mixture was stirred at room temperature for 6 hours and the solvent was evaporated under reduced pressure. The residue was dissolved in ethyl acetate. This solution was washed with diluted hydrochloric acid and saturated aqueous sodium hydrogencarbonate solution and dried over sodium sulfate. The solvent was evaporated under reduced pressure and to the residue was added diisopropyl ether. The precipitated crystals were collected by filtration and washed with hexane to give N-(2-hydroxyphenyl)-2-bromo-5-methyl-3-thiophenecarboxamide (5.5 g). 1 H-NMR(400MHz, CDCl 3 ) δ: 2.45(3H, s, CH 3 ), 6.92(1H, t, ArH), 7.06(1H, d, ArH), 7.16(3H, m, ArH, H of thiophene ring), 8.53, 8.63(1H, 1H, br, br, NH, OH) Starting Material Synthesis Example 68 2-Bromo-3-thiophenecarboxylic acid (30 g) was suspended in thionyl chloride (35 ml) and the mixture was stirred under reflux with heating for 1 hour. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (10 ml). This toluene solutioin was added dropwise to a solution of 2-aminophenol (19 g) in pyridine (100 ml) at 0°C. The mixture was stirred at room temperature for 20 minutes and the solvent was evaporated under reduced pressure. The residue was dissolved in ethyl acetate. This solution was washed with diluted hydrochloric acid and saturated aqueous sodium hydrogencarbonate solution, and the precipitated crystals were collected by filtration to give N-(2-hydroxyphenyl)-5-bromo-3-thiophenecarboxamide (21 g). The filtrate was concentrated and the precipitated crystals were collected by filtration to give N-(2-hydroxyphenyl)-2-bromo-3-thiophenecarboxamide (10 g). 1 H-NMR(400MHz, DMSO-d 6 ) δ 6.82(1H, t, J=7.8Hz, ArH), 6.91(1H, d, J=8.3Hz, ArH), 7.00(1H, t, J=7.8Hz, ArH), 7.40(1H, d, J=5.3Hz, H of thiophene ring), 7.68(1H, d, J=5.9Hz, H of thiophene ring), 7.81(1H, d, J=7.8Hz, ArH), 9.30(1H, br, NH or OH), 9.87(1H, br, NH or OH) Starting Material Synthesis Example 69 2-Bromo-5-methyl-3-thiophenecarboxylic acid (3 g) was suspended in thionyl chloride (15 ml) and the mixture was stirred under reflux with heating for 40 minutes. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (5 ml). This toluene solution was added dropwise to a solution of 2-amino-4-fluorophenol (1.7 g) in pyridine (20 ml) at 0°C. The mixture was stirred at room temperature for 1.5 hours and the solvent was evaporated under reduced pressure. To the residue was added water-ethyl acetate. The precipitated crystals were collected by filtration to give N-(3-fluoro-6-hydroxyphenyl)-2-bromo-5-methyl-3-thiophenecarboxamide (2.1 g). The filtrate was extracted three times with ethyl acetate and the obtained organic layer was washed with water. The organic layer was dried over sodium sulfate and the solvent was evaporated under reduced pressure. To the residue was added ethyl acetate and the precipitated crystals were collected by filtration to give N-(3-fluoro-6-hydroxyphenyl)-2-bromo-5-methyl-3-thiophenecarboxamide (1.3 g). melting point 210-212°C. Starting Material Synthesis Example 70 2-Bromo-4,5-dimethyl-3-thiophenecarboxylic acid (2.9 g) was suspended in thionyl chloride (15 ml) and the mixture was stirred under reflux with heating for 65 minutes. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (25 ml). This toluene solution was added dropwise to a solution of 2-aminophenol (1.5 g) in pyridine (30 ml) at 0°C. The mixture was stirred at room temperature for 1.5 hours and the solvent was evaporated under reduced pressure. To the residue was added ethyl acetate and the mixture was washed with diluted hydrochloric acid, and water and saturated brine. The organic layer was dried over magnesium sulfate and the solvent was evaporated under reduced pressure. To the residue was added dilsopropyl ether and the precipitated crystals were collected by filtration to give N-(2-hydroxyphenyl)-2-bromo-4,5-dimethyl-3-thiophenecarboxamide (3.3 g). 1 H-NMR(400MHz, CD 3 OD) δ 2.20(3H, s, CH 3 ), 2.33(3H, s, CH 3 ), 6.90(2H, m, ArH), 7.03(1H, m, ArH), 7.87(1H, m, ArH) Starting Material Synthesis Example 71 2-Bromo-5-methyl-3-thiophenecarboxylic acid (3 g) was suspended in thionyl chloride (15 ml) and the mixture was stirred under reflux with heating for 70 minutes. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (20 ml). This toluene solution was added dropwise to a solution of 2-amino-5-methylphenol (1.7 g) in pyridine (20 ml) at 0°C. The mixture was stirred at room temperature for 2 hours and the solvent was evaporated under reduced pressure. Thereto was added diluted hydrochloric acid-ethyl acetate. The precipitated crystals were collected by filtration to give N-(2-hydroxy-4-methylphenyl)-2-bromo-5-methyl-3-thiophenecarboxamide (3.3 g). melting point 210-212°C. Starting Material Synthesis Example 72 2-Bromo-5-methyl-3-thiophenecarboxylic acid (2 g) was suspended in thionyl chloride (10 ml) and the mixture was stirred under reflux with heating for 50 minutes. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (20 ml). This toluene solution was added dropwise to a solution of 2-amino-4-methylphenol (1.1 g) in pyridine (20 ml) at 0°C. The mixture was stirred at room temperature for 2.5 hours and the solvent was evaporated under reduced pressure. Thereto was added ethyl acetate-diisopropyl ether. The precipitated crystals were collected by filtration to give N-(2-hydroxy-5-methylphenyl)-2-bromo-5-methyl-3-thiophenecarboxamide (2.6 g). melting point 173-175°C. Starting Material Synthesis Example 73 2-Bromo-5-methyl-3-thiophenecarboxylic acid (3.5 g) was suspended in thionyl chloride (17 ml) and the mixture was stirred under reflux with heating for 1 hour. Thionyl chloride was evaporated under reduced pressure and the residue was dissolved in toluene (20 ml). This toluene solution was added dropwise to a solution of 2-amino-4-chlorophenol (2.3 g) in pyridine (35 ml) at 0°C. The mixture was stirred at room temperature for 1.5 hours and the solvent was evaporated under reduced pressure. To the residue was added ethyl acetate-diisopropyl ether and the precipitated crystals were collected by filtration and washed with diluted hydrochloric acid and then diisopropyl ether to give N-(3-chloro-6-hydroxyphenyl)-2-bromo-5-methyl-3-thiophenecarboxamide (4.1 g). melting point 232-235°C. Example 153 Potassium tert-butoxide (718 mg) was suspended in 1,2-dimethoxyethane (35 ml) and the mixture was refluxed under heating. Thereto was added dropwise ethyl 2-(2-aminoanilino)benzo[b]thiophene-3-carboxylate (1.0 g) dissolved in 1,2-dimethoxyethane (35 ml) over 15 minutes. After 30 minutes, potassium tert-butoxide (359 mg) was added and the mixture was refluxed under heating for 1 hour. After the completion of the reaction, the reaction mixture was allowed to cool to room temperature and poured into 0.5N hydrochloric acid. The mixed solution was extracted 3 times with chloroform, and washed with water and dried over magnesium sulfate. The solution was filtered and the solvent was evaporated under reduced pressure. The precipitated crude crystals were washed with ethyl acetate and diisopropyl ether to give 6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one (300 mg). 1 H-NMR(400MHz, CDCl 3 ) δ 7.10-7.18(3H, m), 7.36-7.44(2H, m), 7.51(1H, d, J=8.8Hz), 7.68(1H, s), 7.88(1H, d=7.8Hz), 7.97(1H, d=7.8Hz), 11.47(1H, br.s). Example 154 In the same manner as in Example 153 and using potassium tert-butoxide and ethyl 2-(2-aminoanilino)-5-fluorobenzo[b]thiophene-3-carboxylate, 8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one was obtained. melting point:249-250°C. MS:m/e=284. 1 H-NMR(270MHz, CDCl 3 ) δ 11.54(1H, brs), 7.97-8.00(1H, m), 7.88-7.92(1H, m), 7.73(1H, s), 7.36-7.47(3H, m), 7.10-7.18(1H, m), 6.96-7.04(1H, m). Formulation Example 1 Of the compounds of the present invention, a compound (50 mg) of the formula (I) is thoroughly kneaded with lactose (98 mg), corn starch (45 mg) and hydroxypropylcellulose (3 mg) in a kneader. The kneaded product is passed through a 200-mesh sieve, dried at 50°C and passes through a 24-mesh sieve. The product is admixed with talc (3 mg) and magnesium stearate (1 mg) and tablets weighing 200 mg are obtained using a pounder having a diameter of 9 mm. The obtained tablets can be sugar coated or film coated as necessary. Experimental Example 1: Effects against MK-801-induced neurotoxicity Female SD rats (about 9-12 weeks old) were orally administered with the inventive compounds or vehicle (0.5% hydroxypropylmethylcellulose, 2 ml/kg). One hour later, they were subcutaneously administered with MK-801 (0.5 mg/kg, 1 ml/kg) dissolved in distilled water. Four hours after the MK-801 administration, the rats were anesthetized with pentobarbital and deblooded with saline, which was followed by perfusion/fixing of brain with formalin solution. Their brains were removed quickly after decapitation, and postfixed in the same formalin solution. The brains were embedded in paraffin and cut into coronal sections through posterior cingulate cortex. The brain sections were stained with hematoxylin/eosin (HE). The number of vacuolized cells was counted by light microscopy and calculated in % of the vehicle-treated group. The inhibitory actions against the MK-801-induced neurotoxicity are to be determined by the potentials to prevent the nerve cell vacuolar reaction by the administration of MK-801, which is expressed as % of the vehicle-treated group. As a result of the test, it was indicated that the compounds of the formula (I) of the present invention suppressed the MK-801-induced neurotoxicity, wherein 50% effective amount to inhibit MK-801-induced neurotoxicity was not more than 10 mg/kg. Experimental Example 2: Inhibitory action against methamphetamine-induced hyperactivity Groups of 15 male ddY mice (20-30 g, 4 weeks old) were used. They were orally administered with the test compounds. One hour later, methamphetamine in saline at 1 mg/kg was subcutaneously administered and the mice were immediately placed in the test box (inside size: 25 × 15 x 14 (height) cm) each fitted with a pair of infra-red beams. Interruptions of the infra-red beams were counted over a 30 min period, from 10 to 40 min after the initiation of observation, and were used as an index of methamphetamine-induced hyperactivity. ED 50 values of the test compounds to suppress the hyperactivity were calculated. As a result of the test, it was indicated that the compounds of the formula (I) of this invention suppressed methamphetamine-induced hyperactivity, wherein 50% effective amount to suppress methamphetamine-induced hyperactivity was not more than 10 mg/kg. Experimental Example 3: Clozapine-cued discrimination test Following reduction, by limiting the diet, to 80% of the body weight under free access to diet, the rats were placed in a Skinner box equipped with two levers. Therein, they were trained to consecutively press 10 times either the right or left lever for feed pellet reward reinforcement. Subsequently, they were injected intraperitoneally with clozapine or saline in a random-alternation sequence once (one session) each day, wherein the drug or saline administration was not given for more than 3 consecutive days. When clozapine was administered at 5 mg/kg, one of the levers was paired with the reward and when saline was administered, the other lever was paired with the reward, whereby the rats were made to acquire discrimination ability. After the acquisition of the discrimination ability, the compound of the present invention was administered to confirm generalization against clozapine. As a result of the test, it was indicated that the compounds of the formula (I) of this invention generalized to the clozapine discrimination stimulus. Experimental Example 4: MK-801-cued discrimination test Following reduction, by limiting the diet, to 80% of the body weight under free access to diet, the rats were placed in a Skinner box equipped with two levers. Therein, they were trained to consecutively press 10 times either the right or left lever for feed pellet reward reinforcement. Subsequently, they were injected intraperitoneally with MK-801 or saline in a random-alternation sequence once (one session) each day, wherein the drug or saline administration was not given for more than 3 consecutive days. When MK-801 was administered at 0.1 mg/kg, one of the levers was paired with the reward and when saline was administered, the other lever was paired with the reward, whereby the rats were made to acquire discrimination ability. After the acquisition of the discrimination ability, the compound of the present invention was administered to confirm antagonism against MK-801 discrimination. As a result of the test, it was indicated that the compounds of the formula (I) of this invention had antagonistic activity against the effect of MK-801 discrimination stimulus. Experimental Example 5: Anti-apomorphine action Groups of 15 male ddY mice (20-30 g, 4 weeks old) were used. They were orally administered with the compounds of the present invention. One hour later, an aqueous solution of apomorphine in ascorbic acid at 0.5 mg/kg was subcutaneously administered and the mice were immediately placed in the test box (inner size: 25×15×14 (height) cm) each fitted with a pair of infra-red beams. Interruptions of the infra-red beams immediately after the initiation of observation to 30 min later were used as an index of apomorphine-induced hyperactivity. The compounds of the formula (I) of this invention suppressed apomorphine-induced hyperactivity, wherein 50% effective amount to suppress apomorphine-induced hyperactivity was not more than 10 mg/kg. Experimental Example 6: Conditioned avoidance response inhibitory action Male Wistar rats (250-400 g, 14-25 weeks old) were placed in the test box (30×30×35 (height) cm) fitted with a pole (diameter 2.5 cm) in its center and grid on the floor and given 5 seconds of sound stimulus (conditioned stimulus) and then 10 seconds of electrical shock (AC 400V, 0.6 mA), thereby forcing them to learn to avoid the electric shock by climbing the pole during the sound stimulus. Each session consisted of 10 sequences of the conditioned stimulus and electric shock at 2-min intervals. The rats (10 per group) capable of stably showing at least 80% on the avoidance response were used for the study of conditioned avoidance response inhibitory action by the compound of the present invention. The conditioned avoidance response at 1, 3, 5 and 24 hours after administration of the compounds of the present invention was determined with the lapse of time and 50% effective amount to inhibit conditioned avoidance response by the compounds of the present invention in each time was determined. As a result of the test, 50% effective amount to inhibit the conditioned avoidance response by the compounds of the formula (I) of this invention was not more than 20 mg/kg. Experimental Example 7: Catalepsy induction Groups of 8 male ddY mice (20-30 g, 4 weeks old) were used for the test. They were administered with the test compounds and the time during which the mice could keep putting their forelimbs over a bar fixed horizontally at 4 cm height with a body posture at an angle of 45° (catalepsy time) was measured up to 30 sec maximum at 1, 3, 5 and 7 hours after the administration. The strength of the catalepsy induction by the test compounds was determined by totalling the time at the four determination points at each dose (sum value) and regressively determining the dose that made the average time 10 sec, based on which ED 10S values were calculated. As a result of the test, the ED 10S value of the compounds of the formula (I) of this invention was not less than 100 mg/kg. Experimental Example 8: Analysis of intermediate in oxidation by horseradish peroxidase A solution (10 µL) of 16 mM inventive compounds in dimethyl sulfoxide, a solution (100 µL) of 5 mM diethylenetriaminepentaacetic acid in phosphate buffer (50 mM, pH 7.4), a solution (100 µL) of 100 mM glutathione in phosphate buffer (50 mM, pH 7.4), 50 mM phosphate buffer (750 µL) and 5,5-dimethyl-1-pyroline N-oxide (1.1 µL) were mixed, and a solution (50 µL) of 0.5 mg/mL horseradish peroxidase in phosphate buffer (50 mM, pH 7.4) was added. The mixture was incubated at 25°C for 4.5 min. The reaction mixture was subjected to ESR spectroscopy. As a result, it was indicated that the compounds of the formula (I) of this invention did not produce cation radical intermediate during oxidation by horseradish peroxidase. From the above-mentioned Experimental Examples, it is suggested that the compounds of the formula of the present invention (IA), showed anti-methamphetamine activity, anti-apomorphine activity, suppression of conditioned avoidance response, suppression of MK-801-induced neurotoxicity, antagonism of MK-801 discrimination and generalization of clozapine discrimination in test animals such as mouse and rats, and are useful as antipsychotics. It is also suggested that they cause less side effects on extrapyramidal system such as catalepsy induction. Furthermore, it was demonstrated that the compounds of the formula (I) of this invention did not produce reaction intermediate corresponding to cation radical intermediate, which is possibly involved in agranulocytosis induced by clozapine, when the reaction intermediate thereof was analyzed using the oxidation by horseradish peroxidase analyzed by ESR spectroscopy as a model reaction of the metabolism of the inventive compounds. The compounds of the formula of the present invention (IA) , are effective for both the positive symptoms and negative symptoms of schizophrenia, are associated with less side effects such as extrapyramidal motor disorder and the like, are less associated with serious side effects such as agranulocytosis, and are useful as novel antipsychotic agents. These compounds are also useful as therapeutic agents for Alzheimer's disease and manic-depressive illness. In addition, the compounds of the formula (IIA) are useful as important synthetic intermediates of the compounds of the formula (IA). This application is based on application Nos. 236700/1997, 277771/1997 and 165725/1998 filed in Japan, the contents of which are incorporated hereinto by reference.

Claims: A condensed thiophene compound of claim 1, wherein the formula (I) is formula (IA) wherein X is NH or -N-R 4 wherein R 4 is alkyl; R 1 and R 2 are the same or different and each is hydrogen, alkyl, alkoxy, hydroxyl or halogen; ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, cycloalkyl, alkoxy, alkoxyalkyl, halogen, alkyl halide, nitro, amino, monoalkylamino, dialkylamino, acylamino, hydroxyl and cyano or a benzene ring without a substituent; ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond; and R 3 is a group of the formula (1), formula (2), formula (3), formula (26), formula (27), formula (28), formula (29) or formula (30) in the formula (1), formula (26), formula (27) and formula (30), R 5 , R 6 and R 7 are the same or different and each is hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl or alkoxyalkyl and a is an integer of 2-4, in the formula (2) and formula (28), R 8 is hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, acyl, alkoxycarbonyl or alkoxyalkyl, b is 1 or 2, in the formula (3) and formula (29), R 9 is hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, aralkyl, hydroxyalkyl, hydroxyalkoxyalkyl, aminoalkyl, monoalkylaminoalkyl, dialkylaminoalkyl, acyl, alkoxycarbonyl or alkoxyalkyl wherein aryl means phenyl or naphthyl and heteroaryl means pyridyl, furyl and thienyl when ever. they occur, or a pharmaceutically acceptable salt thereof or a hydrate thereof. The condensed thiophene compound of claim 1, wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, the ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, and R 3 is a group of the formula (2) wherein R 8 is hydroxyalkoxyalkyl, methyl or ethyl and b is 1, or a pharmaceutically acceptable salt thereof or a hydrate thereof. The condensed thiophene compound of claim 1, wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, the ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen and alkyl halide, or a benzene ring without a substituent, the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond, and R 3 is a group of the formula (2) wherein R 8 is methyl or ethyl and b is 1, or a pharmaceutically acceptable salt thereof or a hydrate thereof. The condensed thiophene compound of claim 1, which is a member selected from the group consisting of 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-methyl-12-[4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-bromo-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 9-methoxy- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8,9-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8,10-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-methyl- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-3-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 2-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-2-methyl- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-methoxy-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 8-fluoro-3-methoxy- 12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine 8,9-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 7,8-dichloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-bromo-8-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-fluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-chloro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 1-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 4-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b] [1,5]benzodiazepine, 3,8-difluoro-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine, 3-fluoro-8-methyl-12-(4-methylpiperazin-1-yl)-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine and 12-(4-ethylpiperazin-1-yl)-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepine or a pharmaceutically acceptable salt thereof or a hydrate thereof. A pharmaceutical agent comprising a condensed thiophene compound of claim 1, a pharmaceutically acceptable salt thereof or a hydrate thereof. The pharmaceutical agent of claim 5, which is an antipsychotic agent. A pharmaceutical composition comprising a condensed thiophene compound of claim 1, a pharmaceutically acceptable salt thereof or a hydrate thereof, and a pharmaceutically acceptable additive. A benzothiophene compound of the formula (IIA) wherein X is NH, or -N-R 4 wherein R 4 is alkyl; R 1 and R 2 are the same or different and each is hydrogen, alkyl, alkoxy, hydroxyl or halogen; ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, cycloalkyl, alkoxy, alkoxyalkyl, halogen, alkyl halide, nitro, amino, monoalkylamino, dialkylamino, acylamino, hydroxyl and cyano or a benzene ring without a substituent; and ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond. The benzothiophene compound of claim 8, wherein X is NH, R 1 and R 2 are the same or different and each is hydrogen, halogen, alkoxy or alkyl, the ring A is a benzene ring having 1 to 4 the same or different substituents thereon selected from the group consisting of alkyl, alkoxy, halogen, hydroxyl and alkyl halide, or a benzene ring without a substituent, and the ring B is a benzene ring wherein the bond shown by a dotted line and a solid line is a double bond. The benzothiophene compound of claim 8, which is a member selected from the group consisting of 6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-bromo-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-bromo-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8,9-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-3-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-3-methoxy-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 2-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8-fluoro-2-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3,9-dimethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 8,9-dichloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 7,8-dichloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 9-trifluoromethyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-bromo-8-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 6-methyl-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-fluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3-chloro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 1-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 4-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one, 3,8-difluoro-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one and 3-fluoro-8-methyl-6H-[1]benzothieno[2,3-b][1,5]benzodiazepin-12(11H)-one.

Inventors: FUJIMURA MASATAKE, HASHIMOTO KENJI, HORIUCHI HIDEKI, KIMURA KOREICHI, KOHARA TOSHIYUKI, SEIO KOJI, TANAKA HIROSHI, YASUMATSU HIROSHI, FUJIMURA, MASATAKE, HASHIMOTO, KENJI, HORIUCHI, HIDEKI, KIMURA, KOREICHI, KOHARA, TOSHIYUKI, SEIO, KOJI, TANAKA, HIROSHI, YASUMATSU, HIROSHI, FUJIMURA, Masatake, Yoshitomi Pharm. Ind., Ltd., HASHIMOTO, Kenji, Yoshitomi Pharm. Ind., Ltd., HORIUCHI, Hideki, Yoshitomi Pharm. Ind., Ltd., KIMURA, Koreichi, Yoshitomi Pharm. Ind., Ltd., KOHARA, Toshiyuki, Yoshitomi Pharm. Ind., Ltd., SEIO, Koji, Yoshitomi Pharm. Ind., Ltd., TANAKA, Hiroshi, Yoshitomi Pharm. Ind., Ltd., YASUMATSU, Hiroshi, Yoshitomi Pharm. Ind., Ltd.
Applicants: MITSUBISHI PHARMA CORP, MITSUBISHI PHARMA CORPORATION
Application year: 1998
Main classification: C07D495
IPC codes: A61K31, C07D498, A61P25, C07D495