IMMUNOGENIC COMPOSITIONS TO THE CCK-B/GASTRIN-RECEPTOR AND METHODS FOR THE TREATMENT OF TUMORS

Abstract: The invention concerns immunogens, immunogenic compositions and method for the treatment of gastrin-dependent tumors. The immunogens comprise a peptide from the CCK-B/gastrin-receptor conjugated to a spacer and to an immunogenic carrier. The immunogens are capable of inducing antibodies in vivo which bind to the CCK-B/gastrin-receptor in tumor cells, thereby preventing growth stimulating peptide hormones from binding to the receptors, and inhibiting tumor cell growth. The immunogens also comprise antibodies against the CCK-B/gastrin-receptor for passive immunization. The invention also concerns diagnostic methods for detecting gastrin-dependent tumors in vivo or from a tissue biopsy using the antibodies of the invention.

Description: IMMUNOGENIC COMPOSITIONS TO THE CCK-B/GASTRIN RECEPTOR AND METHODS FOR THE TREATMENT OF TUMORS BACKGROUND OF THE INVENTION The hormone gastrin binds to a gastrin/cholecystokinin (CCK)-B receptor with high affinity via its 5 carboxy-terminal amino acids. The CCK- B/gastrin receptor is a cytoprasmic membrane protein which is coupled via a G protein to intracellular signal transduction pathways that in turn control the expression of various genes. Gastrin is a peptide hormone which occurs in two forms, tetratriacontagastrin (G34) and heptadecagastrin (G17), and is synthesized and secreted by specialized cells, G cells, that are located in the stomach antrum. The hormone is secreted into the circulating blood and binds to specific cells in the stomach, namely, enterochromaffm-like (ECL) and parietal cells, that indirectly or directly affect stomach acid output. Historically, gastrin hormones have been associated with the stimulation of gastric acid secretion (Edkins, J.S. 1905). (The full citations for the references cited herein are provided in the Reference section preceding the claims.) In recent years, evidence has accumulated that gastrin may act as a trophic factor within the gastrointestinal tract (Johnson, L. 1997) and that it can promote the growth of gastrointestinal cancers (Watson et al. 1989, Dickinson, C.J. 1995), as well as non-gastrointestinal cancers including small cell carcinoma ofthe lung (Rehfeld et al. 1989). In the post-translational processing of gastrin, it is the "mature" carboxy-amidated form that binds to the gastrin/CCK-B receptor via the carboxy terminus (Kopin et al. 1992). It has been shown that several types of tumors, e.g., colorectal, stomach, pancreatic and hepatocellular adenocarcinomas possess CCK-B/gastrin receptors in their plasma membranes and that they respond to gastrin with powerful cellular proliferation (Rehfeld, J.F. 1972, Upp et al. 1989 and Watson et al. 1993) . Furthermore, more recently it has been discovered that many of these cancer cells also secrete gastrin and thus effect an autonomous proliferative pathway (Van- Solinge et al 1993, Nemeth et al. 1993 and Seva et al. 1994) The CCK-B/gastrin receptor belongs to a family of G protein-coupled receptors with seven transmembrane domains with equal affinity for both CCK and gastrin (Soil et al. 1984). This receptor was named a CCK type-B receptor because it was found predominantly in the brain (Wank et al 1992) The receptor was subsequently found to be identical to the peripheral CCK/gastrin receptor in the parietal and ECL cells ofthe stomach (Nakata et al 1992) This receptor has been well characterized in a number of normal (Fourmy et al 1984, Grider et al 1990) and tumor tissues (Singh et al 1990, Watson et al 1993), and extensively studied using the rat pancreatic adenocarcinoma cell line AR42J (Scemama et al 1987) The AR42J CCK-B/gastrin receptor cDNA has been cloned and sequenced, and it is more than 90% homologous in DNA sequence to the CCK-B/gastrin receptor in rat and human brain, and more than 84% homologous in sequence to the canine parietal cell CCK-B/gastrin receptor cDNA (Wank, S.A 1995), demonstrating a high sequence homology even between species. The peptide hormones Gl 7 and G34 bind to the CCK- B/gastrin receptor on the cell membrane of normal cells However, it has been found that G17, and not G34, stimulates the growth of gastrin-dependent cancer cells. Serum-associated G17, in particular, has the potential to stimulate the growth of colorectal tumors in an endocrine manner mediated by CCK-B/gastrin receptors (Watson et al 1993) in the tumor cells. Gastrin- 17 appears to be particularly implicated in stimulating the growth of colorectal adenocarcinomas due to a possible increased affinity for the CCK-B/gastrin receptor on the tumor cells, over other gastrin hormone species (Rehfeld 1972 and 1993) The CCK-B/gastrin receptors were found to be expressed in a high affinity form on 56.1% of human primary colorectal tumors (Upp et al 1989). It has been postulated that a potential autocrine loop may also exist due to endogenous production of precursor gastrin peptides by such tumors (Van-Solinge et al. 1993 and Nemeth et al. 1993). The resulting G17 ligand/receptor complex stimulates cell growth by way of secondary messengers for regulating cell function (Ullrich et al. 1990). The binding of G17 to the CCK- B/gastrin receptor leads to activation of phosphatidyl inositol breakdown, protein kinase C activation with a resultant increase in intracellular calcium ion concentration, as well as the induction of c-fos and c-jun genes via mitogen-activated protein kinase, which has been implicated in the regulation of cell proliferation (Tadisco et al. 1995). Additionally, gastrin binding to the CCK-B/gastrin receptor has been associated with the subsequent increase in phosphorylation by a tyrosine kinase, ppl25FADK (focal adhesion kinase), which may also have a role in the transmission of mitogenic signals (Tanaguchi et al. 1994). A number of high affinity CCK-B/gastrin receptor antagonists have been evaluated therapeutically both in vitro and in vivo in a number of experimental gastrointestinal cancers. For example, proglumide, a glutamic acid derivative (Seva et al. 190; Harrison et al. 1990 and Watson et al. 1991a); Benzotript, an N-acyl derivative of tryptophan; L-365,260, a derivative of Aspercillin (Bock et al. 1989), and CI-988 a molecule that mimics the C-terminal pentapeptide sequence of CCK (Hughes et al. 1990) have been shown to effectively neutralize the effects of exogenous gastrin on gastrointestinal tumor growth both in vitro and in vivo (Watson et al. and Romani et al. 1994). However, these antagonists have severe toxic side effects and lack specificity as they block the action of all potential ligands ofthe receptor such as G34 and CCK in normal cells. Recently, highly potent and selective CCKB/gastrin receptor antagonists such as YM022 (Yuki et al., 1997) and YF476 (Takinami et al, 1997) have been also described. Proglumide and Benzotript have been widely assessed in the pre-clinical studies. The main problem with these compounds is their lack of potency, with relatively high concentrations required to displace G17 (Watson et al., 1992a; Watson et al., 1992b). Despite this, proglumide and benzotript inhibited the basal and gastrin-stimulated proliferation of a number of cell lines (Seva et al., 1990; Watson et al., 1991a). In addition, proglumide increased the survival of xenograft mice bearing the gastrin-sensitive mouse colon tumor, MC26 to 39 days in the treated animals from 25 days in the control animals. Due to the low specificity of this class of gastrin antagonising agents for the gastrin/CCKB receptor, the inhibition of tumor growth may not be effectively control with gastrin antagonists. Moreover, the cellular receptors which recognize and bind the gastrins do not bind all the inhibitors tested (Seva et al. 1994). Thus, if complete inhibition of gastrin binding to the receptor does not occur in the autocrine growth cascade, then the gastrin antagonists may be unable to block this mechanism of tumor growth promotion. SUMMARY OF THE INVENTION The present invention provides immunogenic compositions and immunological methods for the treatment of gastrin-dependent tumors. The method comprises the active or passive immunization of a patient with an anti-CCK-B/gastrin receptor immunogen or anti-CCK- B/gastrin receptor antibodies. The antibodies produced by the immunogens are specific against the CCK-B/gastrin receptor on tumor cells and block the growth-promoting effects of gastrin on the receptors. The antibodies prevent the peptide hormones from binding to the CCK- B/gastrin receptors on gastrin-dependent tumor cells; thus, the growth ofthe tumor is arrested. Furthermore, surprisingly, the antibodies specific to the NH -terminal end ofthe receptor, upon binding to the receptor, are internalized and rapidly translocated into the cytoplasm and into the nucleus ofthe tumor cells. This internalization can occur as early as 10 seconds after exposing the cells to the antibody. This rapid internalization of the antibody/receptor complex in turn causes the affected tumor cells to undergo apoptosis or suicide. The immunogens ofthe invention comprise natural or synthetic peptides derived from the human CCK-B/gastrin receptor, as the immunomimic portion ofthe immunogen. The immunogens may also comprise a spacer peptide sequence attached to an end ofthe immunomimic peptide. The immunogen may also be conjugated to a protein carrier, such as Diphtheria toxoid, tetanus toxoid, bovine serum albumin and the like. In one embodiment, the method of immunization against the CCK- B/gastrin receptor comprises active immunization, wherein a patient is immunized with an immunogen of the invention. The immunogen stimulates the production of antibodies against the CCK-B/gastrin receptor on tumor cells. The antibodies produced by the anti-CCK-B/gastrin receptor immunogens bind to the CCK- B/gastrin receptors on tumor cells and effectively prevent the binding ofthe peptide hormones to the receptors, thereby inhibiting the autocrine growth- stimulatory pathway of tumor cell division and ultimately the growth of the tumor. In another embodiment ofthe invention, the method of treatment comprises passive immunization, whereby antibodies against the CCK-B/gastrin receptor are administered to a patient in a sufficient concentration to bind to the CCK-B/gastrin receptors ofthe tumor cells, and the antibodies block the binding ofthe peptide hormones to the receptor. The prevention of binding ofthe hormones to their receptor inhibits the growth- stimulus pathway ofthe tumor cells, thereby inhibiting the growth ofthe hormone-dependent tumors. In a preferred embodiment of this aspect ofthe invention, the antibodies for human therapy may be chimeric, humanized, or human monoclonal antibodies which may be produced by methods well known in the art. In addition, the anti-CCK-B/gastrin receptor antibodies may be further conjugated to cytotoxic molecules such as cholera toxin, or to radioactive molecules labeled with a radionuclide. such as 125 I and 131 I, to enhance the killing ofthe tumor cells. The invention also provides a method for diagnosing a gastrin-responsive tumor, comprising the immuno chemical detection of gastrin-dependent (CCK-B/gastrin-containing) tumors from a tissue biopsy using the antibodies ofthe invention The specific anti-CCK- B/gastrin receptor antibodies ofthe invention can be labeled with a detection system utilizing compounds such as biotin, horseradish peroxidase and fluorescein to detect the CCK-B/gastrin receptors in the tumor tissue using standard immunochemical procedures The invention also provides a method for diagnosing a gastrin-dependent tumor, comprising the in vivo detection of gastrin-dependent (CCK-B/gastrin receptor-containing) tumors, using the anti-CCK-B/gastrin receptor antibodies The method comprises, administering to a patient possessing a colorectal tumor an effective dose of radiolabeled anti-CCK-B/gastrin receptor antibodies via an intravenous injection, and imaging or detecting tumor cells having anti- CCK-B/gastrin receptor antibodies bound to their cell membranes by standard scintigraphic scanning procedures In this aspect ofthe invention, the anti-CCK-B/gastrin-receptor-antibodies should be labeled with a radionuclide such as "'indium, 90 Yttrium, and 131 I. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1A and IB illustrate schematic views ofthe CCK-B/gastrin receptor and its 7 transmembrane domains. FIG. 2 shows data from ELISA assays with antibodies raised in rabbits immunized with an immunogen against Peptide 1 ofthe CCK-B/gastrin receptor. FIG. 3 shows data from ELISA assays with antibodies raised in rabbits immunized with an immunogen against Peptide 4 ofthe CCK-B/gastrin receptor. FIG. 4 is a graph showing data obtained from an inhibition ELISA used to assess the specificity of affinity-purified antibodies raised against GRP1-DT immunogen. FIG. 5 is a bar graph showing data on the inhibition ofthe binding of 125 I-human Gl 7 to AR42J cells by peptide inhibitors. FIG. 6 is a bar graph ofthe cellular distribution of immunogold-labeled AR4-2J tumor cells. FIG. 7 is a photograph of a Western blot analysis of protein extracts from nuclear membranes of adenocarcinoma cells using antibodies raised against Peptide 1. FIG. 8 is a photograph of a Western blot analysis of protein extracts from extranuclear and plasma membranes of adenocarcinoma cells using antibodies raised against Peptide 1. FIG. 9 is a plot graph illustrating the C170HM2 tumor weight of control and anti-CCK-B/gastrin receptor-treated animals. FIG. 10 is a plot graph illustrating the cross-sectional area of C170HM2 tumors from control and anti-CCK-B/gastrin receptor-treated animals. FIG. 11 is a bar graph showing the mean C170HM2 tumor weights of control and anti-CCK- B/gastrin receptor-treated animals. FIG. 12 is a bar graph showing the mean cross-sectional area of C170HM2 tumors of control and anti-CCK-B/gastrin receptor-treated animals. FIG. 13 is a bar graph showing the mean number of C170HM2 tumors in control and anti- CCK- B/gastrin receptor-treated animals. FIG. 14 is a bar graph showing the median C170HM2 tumor weight of liver metastases, of control and anti-CCK- B/gastrin receptor-treated animals. FIG. 15 is a bar graph showing the median cross-sectional area of C170FDM2 tumors from control and anti-CCK-B/gastrin receptor-treated animals. FIG. 16 is a bar graph showing the median C170HM2 tumor number in control and anti-CCK- B/gastrin receptor-treated animals. FIG. 17 is a bar graph showing the mean and median liver C 170HM2 tumor number in control and anti-CCK-B/gastrin-receptor-treated animals. FIG. 18 is a bar graph showing the mean and median liver C170HM2 tumor weight in control and anti-CCK-B/gastrin-receptor-treated animals. FIG. 19 is a bar graph showing the mean and median values for the cross-sectional area of C170HM2 liver tumor metastases in control and anti-CCK-B/gastrin-receptor-treated animals. FIG. 20 depicts a graph showing the concentration of radiolabeled 125 I-antibodies in C170HM2 liver tumor xenografts of control (normal rabbit serum) and anti-GRPl -treated nude mice. FIG. 21 depicts a bar graph showing the mean C170HM2 liver tumor number per liver of xenografts of control and anti-GRPl -treated nude mice. FIG. 22 depicts a bar graph showing the mean Cl 70HM2 liver tumor weight of liver xenografts of control and anti-GRPl -treated nude mice. FIG. 23 depicts Western blots of C170HM2 liver tumor xenograft proteins of control and anti- GRPl -treated nude mice. FIG. 24 is a photograph of a histological section taken with a light microscope showing a hematoxylin/eosin-stained section of a C170HM2 liver xenograft of a control mouse. FIG. 25 is a photograph of a histological section taken with a light microscope showing a hematoxylin/eosin stained section of a C170HM2 liver xenograft from a mouse treated with rabbit anti-GRPl antibodies. DETAILED DESCRIPTION OF THE INVENTION The methods ofthe invention are directed to the treatment of gastrin hormone- dependent tumors in animals, including humans, and comprise administering to a patient an anti- CCK-B/gastrin-receptor immunogen, which produces antibodies in the immunized patient which bind to the CCK-B/gastrin-receptor on the tumor cells, so as to prevent the binding ofthe hormone to the receptor in order to inhibit the growthpromoting effects ofthe hormone. More importantly, from a clinical point of view, the receptor/anti GRP1 complex is rapidly internalized, traverses the cytoplasm and enters the nucleus. This apparently triggers the affected tumor cells to commit suicide (apoptosis). The immunogens comprise natural or synthetic peptides ofthe human CCK- B/gastrin-receptor which act as immunomimics. In particular, two synthetic peptides have been developed as the immunomimics. These peptides, developed from the amino acid sequence ofthe CCK-B/gastrin-receptor, are immunogenic and cross-reactive with the endogenous CCK- B/gastrin-receptor of tumor cells both in vivo and in vitro. Peptide 1 consists of amino acids 5 through 21 of the CCK- B/gastrin-receptor sequence: KLNRS VQGTGPGPGASL (Peptide 1 , SEQ ID NO.: 1 in the Sequence Listing). Peptide 1 constitutes the amino-terminal end ofthe receptor and is located on the extracellular surface ofthe cell membrane (see FIG. 1). In another embodiment, the immunogen comprises Peptide 4, which consists ofthe following amino acid sequence ofthe CCK-B/gastrin-receptor: GPGAHRALSGAPISF (Peptide 4, SEQ ID NO.: 2 in the Sequence Listing). Peptide 4 is part ofthe fourth extracellular domain of the receptor and it too is on the outer side ofthe plasma membrane (see FIG. 1). The immunogens may also comprise an extension or spacer peptide suitable for projecting the immunomimic peptide away from the protein carrier and enhancing its capacity to bind the lymphocyte receptors. A suitable spacer peptide sequences the amino acid sequence SSPPPPC (Serine (Ser) spacer, SEQ ID NO.:3 in the Sequence Listing). However other spacer peptides would be suitable as well. The immunomimic peptides, with or without the spacer, are then conjugated to a protein carrier, such as Diphtheria toxoid, via a cysteine residue at the carboxy terminal end. The spacer peptides are not immunologically related to the CCK-B/gastrin- receptor-derived peptides and should therefore enhance, but not determine, the specific immunogenicity ofthe receptor-derived peptides. The presence and density of CCK-B/gastrin-receptors on tumor cells in a patient can be determined by reacting labeled anti-receptor antibodies with a sample of obtained from tumor biopsy sample. The anti-receptor antibodies can be labeled with either a radioactive tracer, a dye or a fluorescent label. In addition, the responsiveness ofthe tumor cells to gastrin can be evaluated in vitro from a tumor biopsy sample ofthe patient using standard techniques. Patients having tumors with biopsy samples positive for the CCK-B/gastrin-receptor antibody assay are typical candidates for treatment by the methods ofthe invention. An effective dosage ranging from 0.001 to 2 mg ofthe immunogenic composition is administered to the patient for the treatment ofthe gastrointestinal cancer. The effective dosage ofthe immunogenic composition should be capable of eliciting an immune response in a patient consisting of effective levels of antibody titer against the CCK-B/gastrin-receptor 1 -3 months after immunization. Following the immunization of a patient, the effectiveness ofthe immunogens is monitored by standard clinical procedures, such as ultrasound and magnetic resonance imaging (MRI), to detect the presence and size of tumors. The antibody titer levels against the receptor may also be monitored from a sample of blood taken from the patient. Booster immunizations should be given as required to maintain an effective antibody titer. Effective treatment of gastrin-dependent cancers, such as stomach, liver, pancreatic and colorectal adenocarcinomas, according to this method should result in inhibition of tumor growth and a decrease in size ofthe tumor. The antibodies raised by the anti-CCK-B/gastrin-receptor immunogens ofthe present invention may have anti-trophic effects against gastrin-dependent tumors by three potential mechanisms: (i) inhibition of gastrin binding to its receptor, (ii) degradation or disruption ofthe signal transduction pathway of tumor cell proliferation; and (iii) induction of apoptosis (or cell suicide) in cells where receptor/antibody complexes are internalized and migrate into the nucleus. In another embodiment ofthe invention, anti-CCK-B/gastrin-receptor antibodies are administered to a patient possessing a CCK-B/gastrin-receptor-responsive tumor. The antibodies specifically bind to the CCK-B/gastrin-receptors on the tumor cells. The binding ofthe antibodies to the receptors prevents the binding of gastrin to its ligand in the membranes of cells and, therefore, the growth signal for the gastrin-dependent tumor cells is inhibited and the growth ofthe tumor is arrested. The antibodies are preferably chimeric or humanized antibodies, or fragments thereof , which effectively bind to the target receptor and may be produced by standard techniques such as those disclosed in U.S. Pat. Nos. 5,023,077, 5,468,494, 5,607,676, 5,609,870, 5,688,506 and 5,662,702. These exogenously produced antibodies may also be useful for killing tumor cells that bear the CCK-B/gastrin-receptor on their plasma membranes by virtue of their inhibiting the growth ofthe tumor cells or delivering a toxic substance to the tumor cell. Preferred anti-CCK-B/gastrin antibodies for therapy are those reactive with extracellular domains 1 and 4 ofthe receptor protein shown in FIG. 1 as GRP-1 and GRP-4, respectively. Particularly preferred are, antibodies which specifically recognize and bind amino acid sequences ofthe receptor protein corresponding to Peptides 1 and 4. The inhibition of tumor growth in this method of immunization is also monitored by ultrasound imaging and MRI and repeated immunizations are administered as required by the patient. The effectiveness ofthe antibodies in inhibiting tumor cell growth and killing of tumor cells can be enhanced by conjugating cytotoxic molecules to the anti-CCK-B/gastrin antibodies. The cytotoxic molecules can be toxins, for example, cholera toxin, ricin, α-amanitin, or radioactive molecules labeled , for example with 1 5 I or 131 I, or chemotherapeutic agents, for example, cytosine arabinoside or 5-fluorouridine In addition to antibodies radiolabeled with 125 I and 131 I, the anti-CCK-B/Gastrin- receptor antibodies can also be labeled with radionuclide such as n 'indium and 90 Yttrium. In this aspect ofthe invention the antibodies are useful for the detection and diagnosing of CCK- B/gastrin-receptor possessing tumors in vivo, by administering these antibodies to the patient, and detecting bound antibodies on CCK-B/gastrin-receptor-containing tumor cells. After allowing the radio-labeled anti-CCK-B/gastrin antibodies to reach the tumor, about 1-2 hours after injection, the radioactive, "hot spots" are imaged using standard scintigraphic procedures as previously disclosed (Harrison's Principles of Internal Medicine, Isselbacher et al. eds. 13 th Ed. 1994). The compositions in which the immunogens are administered for the treatment of gastrin-dependent tumors in patients may be in a variety of forms. These include, for example, solid, semi-solid and liquid dosage forms, such as powders, liquid solutions, suspensions, suppositories, and injectable and infusible solutions. The preferred form depends on the intended mode of administration and therapeutic applications. The compositions comprise the present immunogens and suitable pharmaceutically acceptable components, and may include other medicinal agents, carriers, adjuvants, excipients, etc. Suitable adjuvants may include nor- muramyl dipeptide (nor-MDP, Peninsula Labs., CA), and oils such as Montanide ISA 703 (Seppic, Inc., Paris, France), which can be mixed using standard procedures. Preferably, the compositions are in the form of unit dose. The amount of active compound administered for immunization or as a medicament at one time, or over a period of time, will depend on the subject being treated, the manner and form of administration, and the judgment ofthe treating physician. The anti-CCK-B/gastrin-receptor antibodies ofthe invention for passive immunization are preferably administered to a patient intravenously using a pharmaceutically acceptable carrier, such as a saline solution, for example, phosphate-buffered saline. EXAMPLE 1 Preparation of GRP1-DT and GRP4-DT Conjugates CCK-B/gastrin-receptor peptides were prepared by standard solid state peptide synthesis. To make immunogens capable of inducing specific immune responses each of Peptide 1 and 4 was synthesized containing the spacer sequence SSPPPPC (SEQ ID NO.:3 in the Sequence Listing) at its carboxy terminus. These peptides were subsequently conjugated to amino groups present on the carrier, Diphtheria toxoid ("DT"), via the terminal peptide amino acid residue cysteine ofthe spacer utilizing a heterobifunctional linking agent containing a succinimidyl ester at one end and maleimide at the other end ofthe linking agent by either of Method A or Method B as described below. Method A: As previously described in U.S. Patent No. 5,023,077, the linking of Peptide 1 or 4 above and the carrier is accomplished as follows. Dry peptide was dissolved in 0.1 M Sodium Phosphate Buffer, pH 8.0, with a thirty-fold molar excess of dithiothreitol ("DTT"). The solution was stirred under a water saturated nitrogen gas atmosphere for four hours. The peptide containing reduced cysteine was separated from the other components by chromatography over a G10 Sephadex column equilibrated with 0.2 M acetic acid. The peptide was lyophilized and stored under vacuum until used. The carrier was activated by treatment with the heterobifunctional linking agent e.g. Epsilon- maleimidocaproic acid N-hydroxysuccinimide ester, ("EMCS"), in proportions sufficient to achieve activation of approximately 25 free amino groups per 10 molecular weight of carrier. In the specific instance of diphtheria toxoid, this amounted to the addition of 6J8 mg of EMCS (purity 75%) to each 20 mg of diphtheria toxoid. Activation of diphtheria toxoid was accomplished by dissolving each 20 mg aliquot of diphtheria toxoid in 1 ml of 0.2 M Sodium Phosphate Buffer, pH 6.45. Aliquots of 6.18 mg EMCS were dissolved into 0.2 ml of Dimethyl Formamide ("DMF"). Under darkened conditions, the EMCS was added dropwise in 50 microliter ("μl") amounts to the DT with stirring. After 2 hours of incubation in darkness, the mixture was chromatographed on a G50 Sephadex column equilibrated with 0J M Sodium Citrate buffer, pH 6.0, containing 0J mM EDTA. Fractions containing the EMCS activated diphtheria toxoid were concentrated over a PM 10 ultrafiltration membrane under conditions of darkness. The protein content ofthe concentrate was determined by either the Lowry or Bradford methods. The EMCS content ofthe carrier was determined by incubation ofthe activated carrier with cysteine-HCl followed by reaction with lOmM of Ellman's Reagent 5,5'dithio-bis (2-nitrobenzoic acid) lOmM. The optical density difference between a blank tube containing cysteine-HCl and the sample tube containing cysteine-HCl and carrier was translated into EMCS group content by using the molar extinction coefficient of 13.6x10 for 5-thio-2-nitrobenzoic acid at 412 nm. The reduced cysteine content (-SH) ofthe peptide was also determined utilizing Ellman's Reagent. Approximately 1 mg of peptide was dissolved in 1 ml of nitrogen gas saturated water and a 0J ml aliquot of this solution was reacted with Ellman's Reagent. Utilizing the molar extinction coefficient of 5-thio-2-nitrobenzoic acid (13.6xl0 3 , the free cysteine -SH was calculated. An amount of peptide containing sufficient free -SH to react with each of 25 EMCS activated amino groups on the carrier was dissolved in OJ M Sodium Citrate Buffer, pH 6.0, containing OJ mM EDTA, and added dropwise to the EMCS activated carrier under darkened conditions. After all the peptide solution had been added to the carrier, the mixture was incubated overnight in the dark under a water-saturated nitrogen gas atmosphere. The conjugate ofthe peptide linked to the carrier via EMCS was separated from other components ofthe mixture by chromatography over a G50 Sephadex column equilibrated with 0.2 M Ammonium Bicarbonate. The conjugate eluted in the column void volume was lyophilized and stored desiccated at 20° C until used. The resulting conjugate may be characterized as to peptide content by a number of methods known to those skilled in the art including weight gain, amino acid analysis, etc. Conjugates of Peptides 1 and 4 with spacer and diphtheria toxoid produced by this method were determined to have an effective peptide/carrier ratio of 5-35 moles of peptide per 100 KD MW of carrier and all were considered suitable as immunogens for immunization of test animals. Preferably, the range ofthe peptide from 10-30 moles per 100 BCD MW of DT produced an effective immune response. Method B: In a preferred method, conjugates comprising GRP1 coupled to DT and GRP4 peptide coupled to DT were prepared at room temperature as follows. Purified DT (400 mg) was dissolved in 20 ml of 0.5 M phosphate buffer, pH=6.6, saturated with nitrogen gas to give a DT solution of 20 mg/ml. The DT solution was placed in a 60 ml dark amber glass bottle (serving as a reaction vessel and filtration reservoir). EMCS coupling reagent (123.6 mg) was dissolved in 2.0 ml of dimethylformamide. The EMCS solution was added dropwise to the DT solution over a 15 minute period with continuous stirring. The bottle was capped, and the mixture was stirred at room temperature for an additional 1 hour 45 minutes, to form activated DT (M-DT). The M-DT was then purified by diafiltration using an Amicon Model TFC10 Thin- Channel Ultrafiltration System per operating manual I-l 13G with a XM50 diaflow ultrafiltration membrane. The M-DT was washed twice against volumes of 420 ml phosphate buffer, concentrating to 20 ml each time, then washed once against 420 ml of OJ M sodium citrate buffer, pH=6.0, containing OJ M EDTA, and concentrating the solution down to 20 ml. To make GRP1-DT conjugate, 2.02 ml of M-DT solution (containing 22.3 mg M- DT) was placed in a 10 ml dark amber glass vial, then 13 mg of GRP1 peptide was dissolved in the citrate buffer to give 40 mg/ml peptide and added dropwise to the M-DT solution with stirring. To make GRP4-DT conjugate, 2.21 ml of M-DT solution (containing 24.4 mg M-DT) was placed in a 10 ml dark amber glass vial, then 13 mg of GRP4 peptide was dissolved in the citrate buffer to give 40 mg/ml peptide and added dropwise to the M-DT solution with stirring. The reactions were allowed to proceed overnight in the dark. Each conjugate was removed from the reaction vessels and separately dialyzed in 12,000-14,000 MW cutoff dialysis tubing against 5 changes 500 ml of 0J M ammonium bicarbonate solution. Each conjugate was lyophilized. The conjugates were then analyzed by amino acid analysis and their peptide to DT substitution ratios were determined to be 21.8 peptides per 10 5 MW of DT for GRP1-DT and 21 J peptides per 10 5 MW of DT for GRP4-DT. Conjugates of Peptides 1 and 4 with spacer and DT produced by this method have an effective peptide/carrier ratio of 5-35 moles of peptide per 100 KD MW of carrier and all are considered suitable as immunogens. A preferred ratio range for producing an effective immune respose is from 10-25 moles of peptide per 100 KD MW of DT. Preparation of Immunogens The present immunogens containing either Peptide 1 or Peptide 4 with spacer conjugated to DT as described above were used to immunize rabbits. Immunogens were prepared as follows: Conjugate was dissolved in 0J5 M Sodium phosphate buffered saline, pH 7.3 to a concentration of 3.79 mg/ml. The conjugate solution was added to Montanide ISA (703) Adjuvant (Seppic, Inc.) in a 30:70 (wt:wt) ratio of conjugate solution to Montanide ISA 703, then the mixture was homogenized using a Silverson Homogenizer for 3 minutes at 8,000 RPM to form an emulsion containing 1 mg/ml of conjugate. Immunization and Sample Collection Rabbits were injected intramuscularly with 0J ml of immunogen consisting of 0J mg of either GRPl-DT, or GRP4-DT conjugate. Each rabbit was given injections of immunogen at 0 and 4 weeks. Blood was collected from each rabbit at 6 and 8 weeks ofthe experiment. Serum was prepared from each blood sample and stored at -20° C until utilized in assays to determine the presence of anti-CCK-B/gastrin-receptor antibodies. Enzyme-Linked Immunosorbent Assay (ELISA) A solid-phase ELISA was used to screen for reaction or cross-reaction of antisera raised against Peptide 1 and Peptide 4 of each immunized rabbit. The ELISA was carried out by coating polystyrene 96 well plates (IMMULON II, Dynatech) with 25 μl/well of 10 μg/ml of Peptide 1 linked to bovine serum albumin (BSA) ("GRP1-BSA"), or Peptide 4 linked to BSA ("GRP4-BSA") antigen in 0J M Glycine- HCI, pH 9.5 buffer. The plates were incubated overnight at 4° C, and subsequently washed in buffer. Antisera obtained from the immunized rabbits were serially diluted to a range of 10 "1 to 10 "8 in 1% BSA-FTA hemagglutination buffer, pH 7.2. Twenty five μl of test antiserum per well was incubated with each test peptide for 1 hr at room temperature. After incubation, the plates were washed thoroughly with buffer to remove any unbound antibody. Each well was treated with 25 μl of biotinylated goat anti-rabbit IgG (H+L) diluted 1 : 1000 in 1% BSA-FTA dilution buffer for 1 hour at room temperature. After washing the plates to remove unbound anti- rabbit reagent, each well was incubated for 1 hour at room temperature with 25 μl of avidin- alkaline phosphatase conjugate diluted 1 : 1000 in 1% BSA-FTA buffer. The plates were washed thoroughly to remove unbound avidin-alkaline phosphatase reagent, and incubated with 25 μl of 1 mg/ml of p-nitrophenylphosphate ("PNPP") in 10% diethanolamine buffer containing 0.01% MgCl 2 .6H 2 O, pH 9.8. The plates were allowed to develop until the absorbance ofthe reaction at 490 nm wavelength reached an optical density between 0.8 to 1.5. To test the specificity ofthe antisera produced by the rabbits, rabbits were also immunized with DT and for ELISA testing, plates were coated with DT as antigen to determine the reactivity ofthe antisera produced against the carrier. FIG. 2 shows the ELISA results using Peptide 1/GRPl and FIG. 3 shows the ELISA results using Peptide 4/GRP4 as the antigen. As seen in FIG. 2, the ELISA results show that the rabbits immunized with Peptide 1-spacer-DT conjugate produced high antibody titers which specifically bind to Peptide 1, as indicated by the antibody binding Peptide 1 even at high (1 : 100,000) dilutions ofthe antiserum. Similarly, FIG. 3 shows that rabbits immunized with Peptide 4-spacer-DT conjugate produced high titers of anti-Peptide 4 antibodies. As seen in FIGs. 2 and 3, the rabbits immunized against each peptide produced antibodies which bound specifically to each peptide at small antisera concentration. The data indicate that the anti-Peptide 1 and anti-Peptide 4 antibodies have a large capacity for binding Peptides 1 and 4 ofthe CCK- B/gastrin-receptor. The data also shows that immunization of rabbits with the present conjugates elicits powerful immune responses against Peptide 1 and Peptide 4, respectively. In addition, rabbits immunized with either Peptide- 1 or Peptide-4 conjugate appeared and behaved normal and did not exhibit any symptoms of disease or pathologies during the experiments. EXAMPLE 2 The following experiments were performed to establish the specificity of antibodies raised in rabbits against the GRPl-DT peptide containing Ser spacer described Example 1 using Method B. A series of tests were conducted to assess the specificity of rabbit antibodies induced by immunization with the GRPl-DT and affinity purified by immunoadsorption over a GRPl-Ser Sepharose column. An inhibition ELISA was used to assess the specificity ofthe affinity purified antibodies for GRPl-Ser peptide. The assays were run as ' follows: GRPl-Ser-BSA conjugate was coated onto 96 well plates (Immulon U bottom) by overnight incubation of 50 μl of a 2 μg/ml solution of conjugate in glycine buffer (OJM, pH=9.5) at 4°C. Affinity purified anti-GRPl Ab (at a final concentration of 10 ng/ml) was combined with various inhibitors (in 1 : 10 dilution series) and incubated for 1 hour at room temperature. The inhibitors included GRPl-Ser, GRPl EPT, Ser, human gastrin 17(l-9)-Ser spacer (hG17(9)-Ser), GRPl EPT + Ser, and buffer (no inhibitor). Incubation buffer consisted of PBS + 0.5% BSA + 0.05% Tween 20 + 0.02% NaN 3 . Subsequent steps used the same buffer without BSA. The 96 well plates were washed free of nonbound GRPl-Ser-BSA, and the Ab + inhibitor mixtures were added (50 μl/well). After 1 hour, the plates were washed and a goat anti-rabbit Ig (H+L) alkaline phosphatase conjugate (Zymed) was added (1 :2000 dilution). After 1 hour incubation, the plates were washed to remove nonbound reagent, and 50 μl/well of pNPP substrate (Sigma) solution (1 mg/ml) was added in substrate buffer (PBS + 0 1 mg/ml MgCl 2 + 10% diethanolamine + 0 02% NaN * ) Following a 60 minute incubation, absorbance was measured on a MRX reader (Dynatech Laboratoπes) Samples were run in duplicate, and means were calculated for each concentration Background binding (established with affinity purified rabbit anti-GnRH antibodies) was subtracted from all values, and the % Inhibition relative to no inhibitor added (anti-GRPl Ab + buffer) was calculated for each inhibitor tested % Inhibition = (100)(l-((A umnhl b 1 ted-A ιnhlb , t ed)/A unιnhlblte d), where A = Absorbance The results are shown in Figure 4 FIG 4 presents the percent inhibition of antibody binding as a function of inhibitor concentration As can be seen in the figure, the GRPl-Ser peptide fully inhibited antibody binding to GRP 1 -Ser-BSA Approximately 60% inhibition was attained with the GRP 1 EPT peptide, which does not contain the Ser spacer sequence, and by an equimolar mixture of GRPl EPT plus Ser spacer The failure of these peptides to produce full inhibition suggests that a proportion of the antibodies were specific for an epιtope(s) compnsing elements of both the GRPl and the Ser spacer sequences No inhibition was obtained by either the Ser spacer sequence itself or by an unrelated peptide bearing the Ser spacer ("hG17(9)-Ser", consisting ofthe amino-terminal nine residues of hGl 7 followed by the Ser spacer) These ELISA results demonstrate that the affinity punfied antibody preparation was specific for the GRPl-Ser peptide, and that 60% ofthe binding activity was directed against the gastπn-receptor epitope component ofthe peptide EXAMPLE 3 AR42J tumor cells (European Collection of Animal Cell cultures, Porton Down, UK) are deπved from a rat pancreatic adenocarcinoma and are known to have well charactenzed CCK-B/gastnn-receptors Thus AR42J were tested to confirm the expression of CCK-B/gastnn- receptor and specificity ofthe receptor for hG17 by radioligand inhibition. AR42J cells were cultured at 37°C with 7% CO 2 in complete RPMI 1640 (Sigma) supplemented with 10% FCS (Gemini Bioproducts), 2 mM glutamine (JRH Biosciences), 1 mM sodium pyruvate (JRH B.) and 50 μg/ml gentamicin (Gemini Bioproducts). The cells were harvested from 175 cm 2 T-flasks (Falcon Plastics) with PBS containing 0.25% EDTA, then washed twice with PBS (no EDTA) by centrifugation (400 X g for 10 min). The cells were kept at 0-4°C for all manipulations. A single cell suspension was prepared in buffer, and the cell concentration was adjusted to 10 6 cells/ml. Aliquots of 1 ml of cell suspension were added to 12X75 mm culture tubes, then the cells were centrifuged and the supernatants discarded. The cells were resuspended in PBS (0.1 ml/tube) containing human G17 (hG17), gonadotropin releasing hormone (GnRH), or no peptide. The peptide concentrations were 1.0 ng/ml, 100 ng/ml and 10 μg/ml. An aliquot of OJ ml of 125 I- hG17 (NEN), containing approximately 26,300 CPM (specific activity, 2200 Ci/mmol), was added to each tube. The tubes were vortexed, then incubated for 15 minutes. The cells were washed twice with PBS, then counted in a γ counter (Wallac). Samples were run in duplicate. Background counts were subtracted, then the % inhibition of 125 I-hGl 7 binding by each inhibitor was calculated using the equation: % Inhibition = (100)(l-((CPM unin ibited- CPMin ibited)/CPMuninhibited) • The results ofthe radioligand binding inhibition tests are shown in FIG. 5, which presents the means (± SE) ofthe individual values. As can be seen in the figure, binding of 125 I- hG17 to AR42J cells was inhibited by hG17. The degree of inhibition increased with the quantity of inhibitor added, to 32% inhibition at lμg hG17 per tube, the highest concentration of peptide tested. Conversely, GnRH produced no inhibition at the two highest concentrations tested (the 6% inhibition obtained with 100 pg GnRH was considered to be nonspecific), indicating that the inhibition by hG17 was specific for gastrin. These results confirmed the cell surface expression of gastrin-receptor by the AR42J tumor cells. EXAMPLE 4 Binding ofthe GRPl-Ser specific antibodies to AR42J cells was assessed by immunofluorescence. AR42J cells were grown as in the previous Examples and harvested with cell scrapers from 175 cm 2 T-flasks and washed twice with buffer (PBS with 0.02% NaN 3 ) by centrifugation (400 X g for 7 min). The cells were kept at 0-4°C for all manipulations. A single cell suspension was prepared in buffer, and the cell concentration was adjusted to 10 6 cells/ml. The cell suspension was added to 1.5 ml microfuge tubes (1 ml/tube). The cells were pelleted by centrifugation and supernatants were aspirated. The cells were resuspended in buffer (0.1 ml/tube) containing peptide inhibitors (1.0 mg/ml). The inhibitors included GRPl-Ser, GnRH, hG17(9)-Ser and buffer (no inhibitor). Antibodies, including the rabbit anti-GRPl-Ser (100 μg/ml), affinity purified rabbit anti-DT (negative control, 100 μg/ml), mouse anti-AR42J antiserum (positive control, 1 : 100 dilution, heat inactivated) or normal mouse serum were added to the appropriate tubes and the contents were mixed. The cells were incubated for 1 hour, with occasional mixing. The cells were then washed three times with buffer, and 0.1 ml of fluorescein- labeled goat anti-rabbit IgG (Antibodies Incorporated) (diluted 1 :50) was added per tube. The cells treated with mouse sera were developed with a fluorescein-anti-mouse IgG reagent (Zymed). The cells were re-suspended by vortexing, then incubated for 1 hour. The cells were again washed three times, then re-suspended in glycerol:PBS (1 : 1, v:v), 50 μl/tube. Wet mounts were prepared with the contents of each tube, and the cells examined using a Laborlux 12 fluorescent microscope (Leitz). Fluorescence was scored on a scale of 0 to 4, with 0 representing background fluorescence (obtained with the normal mouse serum) and 4 representing maximal fluorescence (obtained with the mouse anti-AR42J positive control antiserum). The results ofthe immunofluorescesce tests are presented in Table 1. As can be seen in the Table, AR42J cells treated with anti-GRPl -Ser antibodies in the absence of peptide inhibitors fluoresced strongly, indicating that the antibody bound to the cells. Rabbit anti-DT antibodies did not produce fluorescent staining, demonstrating that the staining observed with the anti-GRPl -Ser antibodies was not a consequence of non-specific cell surface binding by rabbit immunoglobulin. Moreover, the binding was shown to be specific for the GRPl-Ser peptide. Addition of GRPl-Ser fully inhibited binding, whereas unrelated peptides, including hG17(9)-Ser and GnRH, failed to inhibit. As the GRPl epitope comprises residues 5-21 ofthe gastrin- receptor, it was concluded that the anti-GRPl -Ser antibodies were specific for the gastrin- receptor expressed by AR42J cells. Table 1 EXAMPLE 5 AR42J cells, passage nos. 16-18 were cultured in RPMI-1640 medium containing 10% FCS and 2 mM glutamine. All cells were maintained at 37 ' C in 5% CO 2 in air at 100% humidity, grown to 80% conflucency in T75 flasks (Falcon, London, UK) and passage following a 0.02% EDTA treatment to bring adherent cells into suspension. Cells were incubated for 10, 30 seconds, 30 minutes and 1 hour with anti-CCK-B/gastrin-receptor antibody (aGR) generated in rabbits with a CCK-B/gastrin Peptide 1 receptor immunogen ofthe invention as described in Example 1 , which had been purified by affinity chromatography in a column prepared with Peptide 1. The cells were fixed in 1% glutaraldehyde for one hour and prepared for immunoelectron microscopy (ImmunoEM) studies using standard techniques. The cell suspensions was centrifuged twice at 2000 rpm for 2 minutes and then the cell pellet resuspended in phosphate buffered saline (PBS). The cell pellet was infiltrated with LRwhite plastic resin. Ultrathin sections of 70-90 nm in thickness were cut and place on Pioloform coated nickel grids. The grids were placed in normal goat serum (Dako, High Wycombe, UK) in 0.1% bovine serum albumin (BSA) (Sigma, Poole, Dorset) and incubated at room temperature for 30 minutes. Grids were rinsed in PBS then incubated with a secondary antibody, biotin-conjugated goat anti-rabbit antibody (gold-labelled), diluted 1:50 in 1% BSA, for 1 hour at room temperature. Control experiments were performed without secondary antibody. After final PBS wash, the grids were counterstained in saturated aqueous uranyl acetate for 3 minutes and Reynold's lead citrate for 3 minutes. Gold particles on the cell membrane, in the cytoplasm, on the nuclear membrane and within the nucleus were counted. Twenty-five cells/grid were counted by an independent observer. For controls AR42J cells were exposed to antibodies for less than 1 second, and liver cells which are devoid of CCK-B/gastrin-receptor were used. AR42J cells exposed to normal IgG were also used as controls for determining non-specific binding ofthe anti-CCK-B/gastrin- receptor antibodies. The results of these experiments are shown in Table 2 and FIG. 6. Table 2 Distribution of CCK-B/gastrin-receptor Immunogold Particles Within AR42J cells (mean ± SEM for 25 cells, repeated n=5.) As demonstrated in Table 2 and FIG. 6, immunogold-antibody particles attached to the CCK-B/gastrin-receptor were localized on plasma membrane, cytoplasm, nuclear membrane, and nuclear matrix ofthe adenocarcinoma cells, further demonstrating that the antibody/receptor complex is internalized by the cells. As seen in Table 2, the immunoEM studies using an antiserum directed against the amino-terminal end ofthe CCK-B/gastrin-receptor shows that after one hour incubation, the distribution of immunogold-labelled CCK-B/gastrin-receptor antibody is quickly internalized as 12% ofthe antibody receptor complex is associated with the cell membrane, 36.6% is within the cytoplasm, 7.9% is in the nuclear membrane and, quite surprisingly, 43.5% is within the cell nucleus. Areas of intense CCK- B/gastrin-receptor immunoreactivity within the nucleus are found on chromatin, which may suggest specific binding sites for regulation ofthe DNA. These electron microscopy studies with anti-immunoglobulin conjugated to gold beads (immmunogold) reveal that an extremely rapid turnover ofthe anti-receptor/receptor complex occurs in the tumor cells; as early as 10 seconds after exposure to antibodies, complexes are detectable in the cell nucleus as seen in FIG. 6. EXAMPLE 6 Adenocarcinoma cell lines, namely AR42J, HCT116, C170HM2, LoVo, ST 16 and MGLVA1, were grown in vitro and harvested as described in Examples 3. Cells from 30 x T-75 flasks were suspended in 5 ml of homogenization buffer (ImM sodium hydrogen carbonate, 2 mM magnesium chloride, 1 nM phenylmethylsulphonyl fluoride, 40 mM sodium chloride, 10 μl leupeptin, 1 μM pepstatin, 5 nM EDTA [Sigma]). Homogenization was carried out by 5 bursts of 5 second duration in a homogenizer. For extranuclear membranes, tissue debris was pelleted by centrifugation at 500g, 7 minutes, 4°C. The pellet was discarded and the supernatant centrifuged at 500g, 4°C to remove further debris. The supernatant was recentrifϊiged at 48,000g, 1 hour, 4°C. The pellet containing the extranuclear membrane preparation was suspended in Tris/NP-40 solution (0.1M TRIZMA 0.5% NONTDET P40 [Sigma Chemical]). For nuclear membrane preparations, following homogenization in a second homogenization buffer (25 mM Tris-HCl, pH 7.4, OJ % TRITON 100, 0.32 M sucrose, 3 mM MgCl 2 , 2 mM EGTA 0J mM spermine tetrahydrochloride, 2 mM PMSF, 10 mM bezomidine hydrochloride, 3 mM EGTA aminoacetonitrile hydrochloride [Sigma]), tissue debris was pelleted by centrifugation at 400 g, for 10 minutes at 4°C. The pellet was resuspended in 55% (0.2 M) sucrose in HPLC water. This mixture was spun at 60,000 g for 1 hour at 4°C. The pellet was washed with 0.4% NONIDET P40 in homogenization buffer without TRITON 100. The pellet was spun at 700 g for 15 min at 4°C and resuspended in homogenization buffer without TRITON 100. Protein content is determined by the Lowry method (using a kit from Pierce). Samples containing 10-15 μg of protein were loaded onto a 8-16% Tris/glycine gradient polyacrylamide gel electrophoresis PAGE (Novex R and D systems) in Tris/glycine buffer and run for 90 minutes at 125 constant volts, 36 mA. The gel was fixed in 10% glacial acetic acid for 1 hour and samples were blotted onto nitrocellulose membrane. The membranes were incubated in 1% BSA for 1 hour, followed by incubation with GRPl antiserum (with and without preabsorption) for 1 hour. Antibody binding were detected by the avidin:biotin-peroxidase complex method using diamino-bezidene as the substrate. The Western blot analysis results using Rabbit-antiserum raised against Peptide 1 (Rabbit anti-GRPl antiserum) are shown in FIG. 7 and FIG. 8. As shown in FIG. 7, the protein molecular weight markers range from 116, 66, 45 and 29 kDa. The blot shows a prominent anti-Peptide 1 immunoreactive band localizing at about 43 kDa in all adenocarcinoma cells studied, i.e., HCT1 16, C170HM2, LoVo, ST16 and MGLVA1, except one (AP5LV). This protein corresponds to a truncated form ofthe CCK- B/gastrin-receptor. Some cell lines (HCT 1 16 and C170HM2) show at least 3 other bands, ranging in molecular weight between 60 and 100 KDa. The data indicate that the anti-CCK- B/gastrin-receptor antibodies can recognize and bind to various isoforms ofthe CCK-B/gastrin- receptor in tumor cells. FIG. 8 shows a Western blot from extranuclear (ENM) and plasma membrane of C170HM2 and HCT116 adenocarcinoma cells. As shown in FIG. 8, adenocarcinoma cell lines tested for ENM CCK-B/gastrin-receptors demonstrate the existence of two strongly stained bands: one about 43 KDa and the other at about 66 KDa. When only the plasma membrane fraction was stained, a single band at about 66 KDa was present. Thus, the Western blot studies confirm the immunoEM results that the CCK-B/gastrin-receptor is present in adenocarcinoma tumor cells, although the immunoEM studies do not distinguish between the isoforms ofthe CCK-B/gastrin-receptor. The data indicate that the present immunogens elicit anti-CCK- B/gastrin-receptor antibodies which can recognize and bind various isoforms ofthe receptor, which would be advantageous for the treatment of these tumors. EXAMPLE 7 C170HM2 adenocarcinoma cells were injected intraperitoneally into nude mice and tumors were allowed to grow in the liver Control mice received an infusion of phosphate buffer saline solution (PBS) and experimental mice received an infusion of one anti-CCK- B/gastrin-receptor antibodies. In Group 1, each mouse was infused daily with 0.5 mg of Rabbit anti-CCK-B/gastrin-receptor antibodies generated against Peptide 1 (Rabbit anti-Peptide 1, Rbt@GRPl) In Group 2, each mouse received daily 0.5 mg of Rabbit anti-CCK-B/gastrin- receptor antibodies generated against Peptide 4 (Rabbit anti-Peptide 4, Rbt@GRP4). The mice were studied for a period of 40 days after antibody infusion, sacrificed and the tumors removed for study The weight, size and cross-sectional area ofthe tumors were assessed by standard techniques The results are shown in FIGs 9 and 10 As seen in FIGs. 9 and 10, implantation ofthe colorectal adenocarcinoma cancer cell line C 170HM2 in mice without treatment results in the rapid growth of large tumor masses, as determined by tumor weight, or tumor size, and the tumor cross-sectional area ofthe tumors However, infusion ofthe animals with Rabbit anti-Peptide 1 or Rabbit anti-Peptide 4 antibodies results in a marked decrease in the number of animals having any detectable tumor, as well as in the weight and size of tumors in animals having them when compared to control The same effect can be seen when mean tumor weight, mean tumor size, or mean tumor number is calculated These data are shown in FIGs. 11, 12 and 13. Further insight into the distribution within the population is gained by calculating the medians of tumor numbers, weight and size. The results are shown in FIGS. 14, 15 and 16. As seen in these figures, the Rabbit anti-Peptide 1 immunogen was consistently more effective than Rabbit anti-Peptide 4 in inhibiting tumor growth. However, both Rabbit anti-Peptide 1 and Rabbit anti-Peptide 4 antibodies did exhibit powerful tumor inhibitory activity as compared to the control treatment EXAMPLE 8 A larger tumor burden was generated in nude mice using the colon cancer cell line C170HM2 by a method as described in Example 7, but with a higher initial cell innoculum The C170HM2 is a liver-invasive xenograft model Control and experimental mice were treated also as described in Example 7. Forty days after antibody infusion, the mice were sacrificed and liver tumors were removed and studied FIGs 17, 18 and 19 show the results of these experiments FIG 17 shows the mean and median liver tumor numbers of control and anti-CCK-B/gastrin-receptor antibody treated animals. The data show that the Rabbit-anti-CCK-B/gastrin-receptor antibodies ("Rabbit@GRP") are effective in inhibiting the growth ofthe metastatic tumors in the liver. There is a statistically significant decrease in mean liver tumor numbers in mice livers using Rabbit anti- Peptide 1 (Student's T test), p=0.0084 and in the median liver tumor number, p=0 0016 (Mann Whitney) when compared to controls Mice treated with anti-Peptide 4 antibodies also show a decrease in mean liver tumor number, however, there was no difference in the mean liver tumor number in this animals when compared to controls. FIG 18 shows that anti-Peptide 1 and anti-Peptide 4 antibodies were also capable of reducing the mean and median tumor weights of liver metastases when compared to control animals The data in FIG 19 show that anti-CCK- B/gastrin-receptor treated mice also had a significant decrease in mean and median cross-sectional area ofthe liver tumors when compared to control animals. The data indicate that the anti-CCK-B/gastrin-receptor antibodies are effective in controlling the spread and growth of a gastrin-dependent colon cancer in the liver, which constitutes the major site of metastatic spread of this cancer. EXAMPLE 9 These studies we carried out to confirm GRPl immunoreactivity on C170HM2 cells. The aim ofthe study was to evaluate tumor localization of antiserum raised against GRPl and to determine its therapeutic effect on the growth of C170HM2 cells within the liver of nude mice. C170HM2 cells were injected intraperitoneally into nude mice as described in Examples 7 above. GRPl antiserum was raised in rabbits. The antiserum was radiolabelled with 125 I and administered to nude mice with established C170HM2 xenografts by a tail vein injection. Control mice received 125 I radiolabelled normal rabbit serum. Mice were terminated at increasing time points following injection of a single dose of 125 I antibodies. Radioactivity was measured as counts per minute per gram of (CPM/g) tissue and the liver/liver tumor ratio calculated. FIG. 20 is a graph which shows the radiolabeled rabbit anti-GRP 1 antibodies bound to liver tumors versus control. As seen in the figure, more rabbit anti-GRPl antibodies are bound to liver tumor tissue when compared to controls. FIG. 20 also shows the liver tumor/liver ratio on the y axis with increasing time on the x axis for both radiolabeled normal rabbit serum and GRP 1 antiserum. The normal rabbit serum achieved a ratio of 1 from day 1 which remained constant until day 5. This indicates the level of radiolabel in the liver tumour and normal liver was equal. The ratio for GRPl antiserum accumulated exponentially approaching 2 by day 5. This indicates radiolabeled GRPl antiserum specifically localizes within C170HM2 liver tumors. EXAMPLE 10 Therapeutic effect of GRPl antiserum on C170HM2 xenografts The C170HM2 tumor xenografts were initiated by intraperitoneal injections of cells. Three different cell inocula were used to generate 3 levels of tumor burden The GRPl antiserum was administered passively by tail vein injection daily from day 0. Therapy was terminated on day 40 Effect of GRPl antiserum on tumor 'take rate' The initial parameter evaluated was mean tumor number within the liver which is shown in FIG 21 The normal rabbit antiserum treated controls are grouped in increasing cell inocula As seen in FIG 21, in the control groups the mean tumor number per liver was between 1 and 3 In the GRP 1 antiserum treated group the mean tumor number per liver was less than 1 for all three cell inocula, which was significant for all 3 experiments (one inoculum, n=18, p=0.003, 2 inocula, n=12, p=0.0001 and 3 inocula, n=20, p=0 0068, Mann Whitney analysis) Effect of GRP 1 antiserum on tumor weight of established tumors FIG. 22 shows the mean tumor weight for the normal rabbit serum treated controls on the left panel for the 3 increasing cell innocula The figure also shows the mean tumor weight of nude mice following treatment with GRPl antiserum the mean liver weight was reduced by 60%) with all 3 cell innocula, which was significant for all 3 experiments (one inoculum, p=0 0016, 2 innocula, p=0 0084, and 3 innocula, p=0.0001, Mann Whitney analysis). GRPl immunoreactivity in C170HM2 xenografts as determined by Western blotting Extra-nuclear membrane proteins were prepared from C170HM2 xenografts from 2/3 experiments These were analyzed by Western blotting using the GRP 1 antiserum. FIG. 23 is a photograph ofthe Western blot showing in the normal rabbit serum-treated xenografts 2 immuno-reactive bands were present at 74 and 50kDa, with the former band showing the strongest immunoreactivity. In the GRP 1 antiserum treated xenografts, there are 2 immuno- reactive bands together with an intermediate band, not seen in the control xenografts or cells grown in vitro. A 50kDa band shows the strongest immunoreactivity. This indicates that in the GRPl antiserum treated xenografts a larger proportion ofthe CCKB/gastrin-receptors may be present as an internalized form. Histological analysis of C170HM2 xenografts FIG. 24 shows a microscopic view of a C170HM2 xenograft invading a liver of a nude mouse. The tumor is generally composed of a necrotic center with a viable leading edge which squashes the hepatocytes as it invades the liver. The degree of apoptosis was measured in the viable leading edge of C170HM2 tumors by the Tunel method with positive cells visualized by in situ hybridization. FIG. 25 shows that apoptotic cells were present in the viable tumor cells in the GRPl antiserum-treated xenografts, but not in the normal rabbit serum-treated tumors. The data show that antiserum raised against the amino terminal epitope ofthe CCKB/gastrin-receptor selectively localizes within liver-invasive C 170HM2 tumors. Neutralization ofthe GRPl epitope induced a significant effect on both tumor Jake rate' and gross tumor burden of tumors that did establish. This tumor-inhibitory effect may be due to (a) a general cytostatic effect induced by blocking the CCKB/gastrin-receptor and/or (b) an indirect effect of targeting an antibody to the nucleus ofthe cell, possibly resulting in apoptosis. 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Watson, Susan A. Grimes, Stephen (ii) TITLE OF INVENTION: Immunogenic Compositions to the - CCK-B/Gastrin Receptor and Methods for the Treatment of Tumors (iii) NUMBER OF SEQUENCES: 3 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Dimitrios T. Drivas, White & Case (B) STREET: 1155 Avenue of the Americas (C) CITY: New York (D) STATE: NY (E) COUNTRY: U.S.A. (F) ZIP: 10036-2787 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.30 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Drivas , Dimitrios T. (B) REGISTRATION NUMBER: 32,218 (C) REFERENCE/DOCKET NUMBER: 1102865-0032 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (212) 819-8200 (B) TELEFAX: (212) 354-8113 (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: not relevant (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (v) FRAGMENT TYPE: N-terminal (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 1 : Lys Leu Asn Arg Ser Val Gin Gly Thr Gly Pro Gly Pro Gly Ala Ser 1 5 10 15 Leu (2) INFORMATION FOR SEQ ID NO : 2 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: not relevant (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: NO (v) FRAGMENT TYPE: internal (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: Gly Pro Gly Ala His Arg Ala Leu Ser Gly Ala Pro lie Ser Phe 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: not relevant (D) TOPOLOGY: linear (ii ) MOLECULE TYPE: peptide (X i ) SEQUENCE DESCRIPTION: SEQ ID NO: 3 Ser Ser Pro Pro Pro Pro Cyε 1 5

Claims: We claim: 1. An immunogen comprising a peptide from the CCK-B/gastrin-receptor conjugated to an immunogenic carrier. 2. The immunogen of claim 1, wherein the peptide has the amino acid sequence KLNRSVQGTGPGPGASL (SEQ ID NO.: 1 in the Sequence Listing) or GPGAHRALSGAPISF (SEQ ID NO.: 2 in the Sequence Listing). 3. The immunogen of claim 1 or 2, further comprising a spacer peptide sequence. 4. The immunogen of claim 3, wherein the spacer peptide sequence is SSPPPPC (SEQ ID NO.: 3 in the Sequence Listing). 5. The immunogen of claim 1, wherein the immunogenic carrier is selected from the group consisting of Diphtheria toxoid, tetanus toxoid and bovine serum albumin. 6. A method for treating a malignant condition caused by gastrin-dependent malignant cell growth comprising administering to an animal in need of such treatment an effective amount of an anti-CCK-B/gastrin-receptor immunogen. 7. The method of claim 6, wherein the immunogen comprises a peptide from the CCKB/gastrin-receptor. 8. The method of claim 7, wherein the peptide has the amino acid sequence KLNRSVQGTGPGPGASL (SEQ ID NO.: 1 in the Sequence Listing) or GPGAHRALSGAPISF (SEQ ID NO.: 2 in the Sequence Listing). 9. The method of claim 8, wherein the immunogen further comprises a spacer peptide sequence. 10. The method of claim 9, wherein the spacer peptide sequence is SSPPPPC (SEQ ID NO.:3 in the Sequence Listing). 11. The method of claim 10, further comprising an immunogenic carrier selected from the group consisting of Diptheria toxoid, tetanus toxoid and bovine serum albumin. 12. A method for treating a gastrin-dependent tumor comprising administering to an animal in need of such treatment an effective amount of anti-CCK-B/gastrin antibodies which recognize and bind to the CCK-B/gastrin-receptors in the tumor cells. 13. The method of claim 12, wherein the antibodies are selected from the group consisting of chimeric, monoclonal and humanized antibodies. 14. The method of claim 12, wherein the anti-CCK-B/gastrin-receptor antibodies recognize and bind the amino acid sequence KLNRSVQGTGPGPGASL (SEQ ID NO.: 1 in the Sequence Listing) or GPGAHRALSGAPISF (SEQ ID NO. : 2 in the Sequence Listing) of the receptor. 15. The method of claim 12 or 14, wherein the antibodies are further conjugated to a cytotoxic molecule. 16. The method of claim 15, wherein the cytotoxic molecule is a toxin or a radioactive molecule. 17. The method of claim 16, wherein the toxin is cholera toxin. 18. The method of claim 16, wherein the radioactive molecule is labeled with 125 I or 131 I. 19. A method for detecting a gastrin-responsive tumor containing CCK-B/gastrin-receptors, comprising exposing an anti-gastrin-receptor antibody to cells isolated from a tumor biopsy sample and detecting the CCK-B/gastrin-receptor in the sample. 20. The method of claim 19, wherein the anti-gastrin-receptor antibody is specific for an amino-terminal peptide ofthe CCK-B/gastrin-receptor. 21. The method of claim 20, wherein the peptide is in the amino-terminal region ofthe CCK- B/gastrin-receptor comprises amino acid residues 5-21. 22. An immunogenic composition comprising an anti-CCK-B/gastrin-receptor immunogen. 23. The composition of claim 22, wherein the immunogen comprises a peptide from the CCKB/gastrin-receptor. 24. The compostion of claim 22, wherein the immunogen comprises antibodies against the CCK-B/gastrin-receptor. 25. A method for diagnosing a gastrin-dependent tumor, comprising administering radiolabeled anti-CCK-B/gastrin-receptor antibodies to a patient possessing a colorectal tumor and imaging the tumor by scintigraphic scanning. 26. The method of claim 25, wherein the anti-CCK-B/gastrin-receptor antibodies are radiolabeled with H 'indium or 90 Yttrium.

Inventors: CAPLIN MARTYN, GRIMES STEPHEN, MICHAELI DOV, WATSON SUSAN A, CAPLIN, MARTYN, GRIMES, STEPHEN, MICHAELI, DOV, WATSON, SUSAN, A.
Applicants: APHTON CORP, APHTON CORPORATION
Application year: 1998
Main classification: A61K39
IPC codes: A61K38, A61K39, A61K51, A61P35, A61P43, C07K14, C07K16, G01N33