NOVEL EXENDIN AGONIST COMPOUNDS

Abstract: Novel exendin agonist compounds are provided. These compounds are useful in treating Type I and II diabetes and conditions which would be benefited by lower plasma glucose and delaying and/or slowing gastric emptying.

Description: NOVEL EXENDIN AGONIST COMPOUNDS This application claims the benefit of U.S. Provisional Application No. 60/055,404, filed August 8, 1997, the contents of which are hereby incorporated by reference in their entirety. Field of the Invention The present invention relates to novel compounds which have activity as exendin agonists. These compounds are useful in treatment of Type I and II diabetes, in treatment of disorders which would be benefited by agents which lower plasma glucose levels and in treatment of disorders which would be benefited with agents useful in delaying and/or slowing gastric emptying. The following description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art to the presently claimed invention, nor that any of the publications specifically or implicitly referenced are prior art to that invention. Exendin The exendins are peptides that are found in the venom of the Gila-monster, a lizard common in Arizona and Northern Mexico. Exendin-3 [SEQ. ID. NO. 1] is present in the venom of Heloder a horridum, and exendin-4 [SEQ. ID. NO. 2] is present in the venom of Heloderma suspectum (Eng, J. , et al . , ■T. Riol . Chem. , 265:20259-62, 1990; Eng., J., et al . , J. Riol . Chem. , 267:7402-05, 1992). The amino acid sequence of exendin-3 is shown in Figure 2. The amino acid sequence of exendin-4 is shown in Figure 3. The exendins have some sequence similarity to several members of the glucagon-like peptide family, with the highest homology, 53%, being to GLP-1 [7-36]NH 2 [SEQ. ID. NO. 3] (Goke, et al . , J. Riol . fhem. , 268:19650-55, 1993). GLP-1 [7-36] NH 2 , also known as proglucagon [78-107] or simply, "GLP-1," has an insulinotropic effect, stimulating insulin secretion from pancreatic β-cells. The amino acid sequence of GLP-1 is shown in Figure 4. GLP-1 also inhibits glucagon secretion from pancreatic α-cells (ørsov, et al . , Diabetes, 42:658-61, 1993; D'Alessio, et al., .T. CΛ i n . Tnvsπ ., 97:133-38, 1996). GLP-1 is reported to inhibit gastric emptying (Willms B, et al . , J 01in Bndocrinol Metab 81 (1) : 327-32, 1996; Wettergren A, et al., n g Pis fir.i 38 (4): 665-73, 1993), and gastric acid secretion. Schjoldager BT, et al . , Dig Pis Sci 34 (5): 7- 03-8, 1989; O'Halloran DJ, et al . , J F.n ocrino 126 (1): 169-73, 1990; Wettergren A, et al . , Dig._D_is_£ i 38 (4): 665-73, 1993). GLP-1 [7-37] , which has an additional glycine residue at its carboxy terminus, also stimulates insulin secretion in humans (ørsov, et al . , Diabetes, 42:658-61, 1993) . A transmembrane G-protein adenylate-cyclase-coupled receptor believed to be responsible for the insulinotropic effect of GLP-1 has been cloned from a β-cell line (Thorens, Proc. Natl. Acad. Sci. USA 89:8641-45 (1992)). Exendin-4 reportedly acts at GLP-1 receptors on insulin- secreting βTCl cells, at dispersed acinar cells from guinea pig pancreas, and at parietal cells from stomach; the peptide is also said to stimulate somatostatin release and inhibit gastrin release in isolated stomachs (Goke, et al . , J. Biol . Chem. 268:19650-55, 1993; Schepp, et al . , Eur. J. Pharmacol., 69:183-91, 1994; Eissele, et al . , , 55:629-34, 1994) . Exendin-3 and exendin-4 were reportedly found to stimulate cAMP production in, and amylase release from, pancreatic acinar cells (Malhotra, R. , et al . , Regulatory Pep ides ,41:149-56, 1992; Raufman, et al . , J. Biol. Chem. 267:21432-37, 1992; Singh, et al . , ReguL_£≤ i_ 53:47-59, 1994) . Based on their insulinotropic activities, the use of exendin-3 and exendin-4 for the treatment of diabetes mellitus and the prevention of hyperglycemia has been proposed (Eng, U.S. Patent No. 5,424,286). Agents which serve to delay gastric emptying have found a place in medicine as diagnostic aids in gastro-intestinal radiologic examinations. For example, glucagon is a polypeptide hormone which is produced by the α cells of the pancreatic islets of Langerhans . It is a hyperglycemic agent which mobilizes glucose by activating hepatic glycogenolysis. It can to a lesser extent stimulate the secretion of pancreatic insulin. Glucagon is used in the treatment of insulin-induced hypoglycemia, for example, when administration of glucose intravenously is not possible. However, as glucagon reduces the motility of the gastro- intestinal tract it is also used as a diagnostic aid in gastro-intestinal radiological examinations. Glucagon has also been used in several studies to treat various painful gastro-intestinal disorders associated with spasm. Daniel, et al . (Br. Med. J. , 3:720, 1974) reported quicker symptomatic relief of acute diverticulitis in patients treated with glucagon compared with those who had been treated with analgesics or antispasmodics . A review by Glauser, et al . , (J. Am. Coll. Emergency Physns, 8:228, 1979) described relief of acute esophageal food obstruction following glucagon therapy. In another study glucagon significantly relieved pain and tenderness in 21 patients with biliary tract disease compared with 22 patients treated with placebo (M.J. Stower, et al . , B . J . Surg . , 69:591-2, 1982) . Methods for regulating gastrointestinal motility using amylin agonists are described in International Application No. PCT/US94/10225, published March 16, 1995. Methods for regulating gastrointestinal motility using exendin agonists are described in a U.S. Patent Application Serial No. 08/908,867. Certain exendin agonists are described in United States Provisional Application No. 60/065,442 filed November 14, 1997 and in United States Provisional Application Serial No. 60/066,029 filed November 14, 1997. SUMMARY OF THE INVENTION According to one aspect, the present invention provides novel exendin agonist compounds which exhibit advantageous properties which include effects in slowing gastric emptying and lowering plasma glucose levels. According to the present invention, provided are compounds of the formula (I) [SEQ. ID. NO. 4] : 1 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa 4 Xaa 5 Xaa s Xaa 7 Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa lx Xaa 12 Xaa 13 Leu Lys Asn Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa 2 is His, Arg or Tyr; Xaa 2 is Ser, Gly, Ala or Thr; Xaa 3 is Asp or Glu; Xaa 4 is Phe, Tyr or naphthylalanine; Xaa 5 is Thr or Ser; Xaa 6 is Ser or Thr; Xaa 7 is Asp or Glu; Xaa 8 is Leu, lie, Val, pentylglycine or Met; Xaa 9 is Leu, lie, pentylglycine, Val or Met; Xaa 10 is Phe, Tyr or naphthylalanine; Xaa n is lie, Val, Leu, pentylglycine, tert- butylglycine or Met; Xaa 12 is Glu or Asp; Xaa 13 is Trp, Phe, Tyr, or naphthylalanine; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently Pro, homoproline, 3Hyp, 4Hyp, thioproline, N- alkylglycine, N-alkylpentylglycine or N-alkylalanine; Xaa 18 is Ser, Thr or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. ID. NOS . 1 or 2. Also included within the scope of the present invention are pharmaceutically acceptable salts of the compounds of formula (I) and pharmaceutical compositions including said compounds and salts thereof . Also provided are compounds of the formula (II) [SEQ. ID. NO. 36] : 1 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa 4 Xaa 5 Xaa 6 Xaa 7 Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa n Xaa 12 Xaa 13 Leu X x Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa x is His, Arg, Tyr or 4-imidazopropionyl ; Xaa 2 is Ser, Gly, Ala or Thr; Xaa 3 is Asp or Glu; Xaa 4 is Phe, Tyr or naphthylalanine; Xaa 5 is Thr or Ser; Xaa 6 is Ser or Thr; Xaa 7 is Asp or Glu; Xaa 8 is Leu, lie, Val, pentylglycine or Met; Xaa 9 is Leu, lie, pentylglycine, Val or Met; Xaa 10 is Phe, Tyr or naphthylalanine; Xaa n is lie, Val, Leu, pentylglycine, tert-butylglycine or Met; Xaa 12 is Glu or Asp; Xaa 13 is Trp, Phe, Tyr, or naphthylalanine; X ± is Lys Asn, Asn Lys, Lys-NH e - R Asn, Asn Lys-NH e -R where R is Lys, Arg, C^C^ straight chain or branched alkanoyl or cycloalkylalkanoyl ; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently Pro, homoproline, 3Hyp, 4Hyp, thioproline, N-alkylglycine, N-alkylpentylglycine or N- alkylalanine; Xaa 18 is Ser, Thr or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. ID. NOS . 1 or 2. Also included within the scope of the presentinvention are pharmaceutically acceptable slats of the compounds of formula (II) and pharmaceutical compositions including said compounds and salts thereof. Definitions In accordance with the present invention and as used herein, the following terms are defined to have the following meanings, unless explicitly stated otherwise. The term "amino acid" refers to natural amino acids, unnatural amino acids, and amino acid analogs, all in their D and L stereoisomers if their structure allow such stereoisomeric forms. Natural amino acids include alanine (Ala) , arginine (Arg) , asparagine (Asn) , aspartic acid (Asp) , cysteine (Cys) , glutamine (Gin) , glutamic acid (Glu) , glycine (Gly), histidine (His), isoleucine (lie), leucine (Leu), Lysine (Lys) , methionine (Met) , phenylalanine (Phe) , proline (Pro) , serine (Ser) , threonine (Thr) , typtophan (Trp) , tyrosine (Tyr) and valine (Val) . Unnatural amino acids include, but are not limited to azetidinecarboxylic acid, 2- aminoadipic acid, 3-aminoadipic acid, beta-alanine, aminopropionic acid, 2-aminobutyric acid, 4-aminobutyric acid, 6-aminocaproic acid, 2-aminoheptanoic acid, 2- aminoisobutyric acid, 3-aminoisbutyric acid, 2-aminopimelic acid, tertiary-butylglycine, 2 , 4-diaminoisobutyric acid, desmosine, 2 , 2 ' -diaminopimelic acid, 2 , 3 -diaminopropionic acid, N-ethylglycine, N-ethylasparagine, homoproline, hydroxylysine, allo-hydroxylysine, 3-hydroxyproline, 4- hydroxyproline, isodesmosine, allo- isoleucine, N- methylalanine, N-methylglycine, N-methylisoleucine, N- methylpentylglycine, N-methylvaline, naphthalanine, norvaline, norleucine, ornithine, pentylglycine, pipecolic acid and thioproline. Amino acid analogs include the natural and unnatural amino acids which are chemically blocked, reversibly or irreversibly, or modified on their N-terminal amino group or their side-chain groups, as for example, methionine sulfoxide, methionine sulfone, S- (carboxymethyl) - cysteine, S- (carboxymethyl) -cysteine sulfoxide and S- (carboxymethyl) -cysteine sulfone. The term "amino acid analog" refers to an amino acid wherein either the C-terminal carboxy group, the N-terminal amino group or side-chain functional group has been chemically codified to another functional group. For example, aspartic acid- (beta-methyl ester) is an amino acid analog of aspartic acid; N-ethylglycine is an amino acid analog of glycine; or alanine carboxamide is an amino acid analog of alanine. The term "amino acid residue" refers to radicals having the structure: (1) -C(0)-R-NH-, wherein R typically is - CH(R')-, wherein R' is an amino acid side chain, typically H or a carbon containing substitutent ; or (2) , wherein p is 1, 2 or 3 representing the azetidinecarboxylic acid, proline or pipecolic acid residues, respectively. The term "lower" referred to herein in connection with organic radicals such as alkyl groups defines such groups with up to and including about 6, preferably up to and including 4 and advantageously one or two carbon atoms. Such groups may be straight chain or branched chain. "Pharmaceutically acceptable salt" includes salts of the compounds of the present invention derived from the combination of such compounds and an organic or inorganic acid. In practice the use of the salt form amounts to use of the base form. The compounds of the present invention are useful in both free base and salt form, with both forms being considered as being within the scope of the present invention. In addition, the following abbreviations stand for the following: "ACN" or "CH 3 CN" refers to acetonitrile . "Boc" , "tBoc" or "Tboc" refers to t-butoxy carbonyl . "DCC" refers to N,N' -dicyclohexylcarbodiimide . "Fmoc" refers to fluorenylmethoxycarbonyl . "HBTU" refers to 2- (lH-benzotriazol-1-yl) - 1,1,3,3, -tetramethyluronium hexaflurophosphate . "HOBt" refers to 1-hydroxybenzotriazole monohydrate . "homoP" or hPro" refers to homoproline. "MeAla" or "Nme" refers to N-methylalanine . "naph" refers to naphthylalanine. "pG" or pGly" refers to pentylglycine. "tBuG" refers to tertiary-butylglycine . "ThioP" or tPro" refers to thioproline. RRTFF PF.qORTPTTON OF THE PRAWINGS Figure 1 depicts the amino acid sequences for certain compounds of the present invention [SEQ. IP. NOS. 5 TO 35] . Figure 2 depicts the amino acid sequence for exendin-3 [SEQ. ID. NO. 1] . Figure 3 depicts the amino acid sequence for exendin-4 [SEQ. ID. NO. 2] . Figure 4 depicts the amino acid sequence for GLP-1 [SEQ. ID. NO. 3] . Figure 5 depicts dose dependent effects of exendin-4 in comparison with compound 1 of Figure 1 [SEQ. ID. NO. 5] on plasma glucose levels in db/db mice. Figure 6 depicts a comparison of effects on gastric emptying of exendin-4, exendin-4 acid and compound 1 of Figure 1 [SEQ. IP. NO. 5] . DETAILED DESCRIPTION OF THE TNVENTTON Preferred Compounds According to the present invention, provided are compounds of the formula (I) [SEQ. IP. NO. 4] : 1 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa, Xaa 5 Xaa 6 Xaa 7 Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa xl Xaa 12 Xaa 13 Leu Lys Asn Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa x is His, Arg or Tyr; Xaa 2 is Ser, Gly, Ala or Thr; Xaa 3 is Asp or Glu; Xaa 4 is Phe, Tyr or naphthylalanine; Xaa 5 is Thr or Ser; Xaa 6 is Ser or Thr; Xaa 7 is Asp or Glu; Xaa 8 is Leu, lie, Val, pentylglycine or Met; Xaa, is Leu, lie, pentylglycine, Val or Met; Xaa 10 is Phe, Tyr or naphthylalanine; Xaa 21 is lie, Val, Leu, pentylglycine, tert- butylglycine or Met; Xaa 12 is Glu or Asp; Xaa 13 is Trp, Phe, Tyr, or naphthylalanine; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently Pro, homoproline, 3Hyp, 4Hyp, thioproline, N- alkylglycine, N-alkylpentylglycine or N-alkylalanine; Xaa 18 is Ser, Thr or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. IP. NOS. 1 or 2. Preferred N-alkyl groups for N-alkylglycine, N- alkylpentylglycine and N-alkylalanine include lower alkyl groups preferably of 1 to about 6 carbon atoms, more preferably of 1 to 4 carbon atoms . Suitable compounds of formula (I) include those having amino acid sequences of SEQ. IP. NOS. 5 to 35. Preferred exendin agonist compounds of formula (I) include those wherein Xaa x is His or Tyr. More preferably Xaa- L is His. Preferred are those such' compounds wherein Xaa 2 is Gly. Preferred are those such compounds wherein Xaa 9 is Leu, pentylglycine or Met. Preferred compounds of formula (I) include those wherein Xaa 13 is Trp or Phe. Also preferred are compounds of formula (I) wherein Xaa 4 is Phe or naphthylalanine; Xaa lx is lie or Val and Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N-alkylalanine. Preferably N- alkylalanine has a N-alkyl group of 1 to about 6 carbon atoms. According to an especially preferred aspect, Xaa 15 , Xaa 16 and Xaa 17 are the same amino acid reside. Preferred are compounds of formula (I) wherein Xaa 18 is Ser or Tyr, more preferably Ser. Preferably Z is -NH 2 . According to one aspect, preferred are compounds of formula (I) wherein Xaa x is His or Tyr, more preferably His; Xaa 2 is Gly; Xaa 4 is Phe or naphthylalanine; Xaa 9 is Leu, pentylglycine or Met; Xaa 10 is Phe or naphthylalanine; Xaa lx is lie or Val; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N- alkylalanine; and Xaa 18 is Ser or Tyr, more preferably Ser. More preferably Z is -NH 2 . According to an especially preferred aspect, especially preferred compounds include those of formula (I) wherein: Xaa- L is His or Arg; Xaa 2 is Gly; Xaa 3 is Asp or Glu; Xaa 4 is Phe or napthylalanine; Xaa 5 is Thr or Ser; Xaa 6 is Ser or Thr; Xaa 7 is Asp or Glu; Xaa 8 is Leu or pentylglycine; Xaa 9 is Leu or pentylglycine; Xaa 10 is Phe or naphthylalanine; Xaa xl is lie, Val or t-butyltylglycine; Xaa 12 is Glu or Asp; Xaa 13 is Trp or Phe; Xaa 14 , Xaa 15 , Xaa 16 , and Xaa 17 are independently Pro, homoproline, thioproline, or N-methylalanine; Xaa 18 is Ser or Tyr: and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. IP. NOS. 1 or 2. More preferably Z is -NH 2 . Especially preferred compounds of formula (I) include those having the amino acid sequence of SEQ. IP. NOS. 5, 6, 17, 18, 19, 22, 24, 31, 32 and 35. According to an especially preferred aspect, provided are compounds of formula (I) where Xaa 9 is Leu, lie, Val or pentylglycine, more preferably Leu or pentylglycine, and Xaa 13 is Phe, Tyr or naphthylalanine, more preferably Phe or naphthylalanine. These compounds will exhibit advantageous duration of action and be less subject to oxidative degration, both in vitro and in vivo, as well as during synthesis of the compound. Also provided are compounds of the formula (II) [SEQ. IP. NO. 36] : 1 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa 4 Xaa 5 Xaa 6 Xaa 7 Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa 1:L Xaa 12 Xaa 13 Leu X x Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa x is His, Arg, Tyr or 4-imidazopropionyl ; Xaa 2 is Ser, Gly, Ala or Thr; Xaa 3 is Asp or Glu; Xaa 4 is Phe, Tyr or naphthylalanine; Xaa 5 is Thr or Ser; Xaa 6 is Ser or Thr; Xaa 7 is Asp or Glu; Xaa 8 is Leu, lie, Val, pentylglycine or Met; Xaa 9 is Leu, lie, pentylglycine, Val or Met; Xaa 10 is Phe, Tyr or naphthylalanine; Xaa 11 is lie, Val, Leu, pentylglycine, tert-butylglycine or Met; Xaa 12 is Glu or Asp; Xaa 13 is Trp, Phe, Tyr, or naphthylalanine; X λ is Lys Asn, Asn Lys, Lys-NH e - R Asn, Asn Lys-NH e -R where R is Lys, Arg, C^C^ straight chain or branched alkanoyl or cycloalkylalkanoyl ; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently Pro, homoproline, 3Hyp, 4Hyp, thioproline, N-alkylglycine, N-alkylpentylglycine or N- alkylalanine; Xaa 18 is Ser, Thr or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. ID. NOS. 1 or 2. Also included within the scope of the presentinvention are pharmaceutically acceptable slats of the compounds of formula (II) and pharmaceutical compositions including said compounds and salts thereof. Suitable compounds of formula (II) include that compound having the amino acid sequences of SEQ. ID. NOS. 37-40. Preferred exendin agonist compounds of formula (II) include those wherein Xaa x is His, Tyr or 4-imidazopropionyl . More preferably, Xaa x is His or 4-imidazopropionyl . Preferred are those compounds of formula (II) wherein Xaa 2 is Gly. Preferred are those compounds of formula (II) wherein Xaa 9 is Leu, pentylglycine or Met. Preferred are those compounds of formula (II) wherein Xaa 13 is Trp or Phe. Preferred are those compounds of formula (II) wherein X x is Lys Asn, or Lys-NH e -R Asn, where R is Lys, Arg, C^-C^ straight chain or branched alkanoyl . Also preferred are compounds of formula (II) wherein Xaa 4 is Phe or naphthylalanine; Xaa 10 is Phe or naphthylalanine; Xaa n is lie or Val and Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N-alkylalanine. According to an especially preferred aspect, Xaa 18 is Ser or Tyr. Preferred are those such compounds wherein Xaa 18 is Ser. Preferably, Z is -NH 2 . According to one preferred aspect, preferred are compounds of formula (II) wherein Xaa 4 is Phe or naphthylalanine; Xaa 10 is Phe or naphthylalanine; Xaa xl is lie or Val, X 1 is Lys Asn, or Lys-NH e -R Asn, where R is Lys, Arg, C- L -C- LO straight chain or branched alkanoyl and Xaa 14 , Xaa ls , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N-alkylalanine. The compounds referenced above form salts with various inorganic and organic acids and bases. Such salts include salts prepared with organic and inorganic acids, for example, HC1 , HBr, H 2 S0 4 , H 3 P0 4 , trifluoroacetic acid, acetic acid, formic acid, methanesulfonic acid, toluenesulfonic acid, maleic acid, fumaric acid and camphorsulfonic acid. Salts prepared with bases include ammonium salts, alkali metal salts, e.g., sodium and potassium salts, and alkali earth salts, e.g., calcium and magnesium salts. Acetate, hydrochloride, and trifluoroacetate salts are preferred. The salts may be formed by conventional means, as by reacting the free acid or base forms of the product with one or more equivalents of the appropriate base or acid in a solvent or medium in which the salt is insoluble, or in a solvent such as water which is then removed in vacuo or by freeze-drying or by exchanging the ions of an existing salt for another ion on a suitable ion exchange resin. Utility The compounds described above are useful in view of their pharmacological properties. In particular, the compounds of the invention are exendin agonists, and possess activity as agents to regulate gastric motility and to slow gastric emptying, as evidenced by the ability to reduce post- prandial glucose levels in mammals. Preparation of Compounds The compounds of the present invention may be prepared using standard solid-phase peptide synthesis techniques and preferably an automated or semiautomated peptide synthesizer. Typically, using such techniques, an α-N-carbamoyl protected amino acid and an amino acid attached to the growing peptide chain on a resin are coupled at room temperature in an inert solvent such as dimethylformamide, N-methylpyrrolidinone or methylene chloride in the presence of coupling agents such as dicyclohexylcarbodiimide and 1-hydroxybenzotriazole in the presence of a base such as diisopropylethylamine . The α-N- carbamoyl protecting group is removed from the resulting peptide-resin using a reagent such as trifluoroacetic acid or piperidine, and the coupling reaction repeated with the next desired N-protected amino acid to be added to the peptide chain. Suitable N-protecting groups are well known in the art, with t-butyloxycarbonyl (tBoc) and fluorenylmethoxycarbonyl (Fmoc) being preferred herein. The solvents, amino acid derivatives and 4-methylbenzhydryl-amine resin used in the peptide synthesizer may be purchased from Applied Biosystems Inc. (Foster City, CA) . The following side-chain protected amino acids may be purchased from Applied Biosystems, Inc.: Boc- Arg(Mts), Fmoc-Arg (Pmc) , Boc-Thr (Bzl) , Fmoc-Thr (t-Bu) , Boc- Ser(Bzl), Fmoc-Ser (t-Bu) , Boc-Tyr (BrZ) , Fmoc-Tyr (t-Bu) , Boc- Lys(Cl-Z), Fmoc-Lys (Boc) , Boc-Glu (Bzl) , Fmoc-Glu (t-Bu) , Fmoc- His(Trt), Fmoc-Asn(Trt) , and Fmoc-Gln (Trt) . Boc-His (BOM) may be purchased from Applied Biosystems, Inc. or Bachem Inc. (Torrance, CA) . Anisole, dimethylsulfide, phenol, ethanedithiol, and thioanisole may be obtained from Aldrich Chemical Company (Milwaukee, WI) . Air Products and Chemicals (Allentown, PA) supplies HF . Ethyl ether, acetic acid and methanol may be purchased from Fisher Scientific (Pittsburgh, PA) . Solid phase peptide synthesis may be carried out with an automatic peptide synthesizer (Model 430A, Applied Biosystems Inc., Foster City, CA) using the NMP/HOBt (Option 1) system and tBoc or Fmoc chemistry (see, Applied Biosystems User's Manual for the ABI 430A Peptide Synthesizer, Version 1.3B July 1, 1988, section 6, pp. 49-70, Applied Biosystems, Inc., Foster City, CA) with capping. Boc-peptide-resins may be cleaved with HF (-5°C to 0°C, 1 hour) . The peptide may be extracted from the resin with alternating water and acetic acid, and the filtrates lyophilized. The Fmoc-peptide resins may be cleaved according to standard methods (Introduction to Cleavage Techniques, Applied Biosystems, Inc., 1990, pp. 6- 12) . Peptides may be also be assembled using an Advanced Chem Tech Synthesizer (Model MPS 350, Louisville, Kentucky) . Peptides may be purified by RP-HPLC (preparative and analytical) using a Waters Delta Prep 3000 system. A C4 , C8 or C18 preparative column (10 μ, 2.2 x 25 cm; Vydac, Hesperia, CA) may be used to isolate peptides, and purity may be determined using a C4, C8 or C18 analytical column (5 μ, 0.46 x 25 cm; Vydac). Solvents (A=0.1% TFA/water and B=0.1% TFA/CH 3 CN) may be delivered to the analytical column at a flowrate of 1.0 ml/min and to the preparative column at 15 ml/min. Amino acid analyses may be performed on the Waters Pico Tag system and processed using the Maxima program. Peptides may be hydrolyzed by vapor-phase acid hydrolysis (115°C, 20-24 h) . Hydrolysates may be derivatized and analyzed by standard methods (Cohen, et aL, The Pico Tag Method: A Manual of Advanced Techniques for Amino Acid Analysis, pp. 11-52, Millipore Corporation, Milford, MA (1989) ) . Fast atom bombardment analysis may be carried out by M-Scan, Incorporated (West Chester, PA) . Mass calibration may be performed using cesium iodide or cesium iodide/glycerol . Plasma desorption ionization analysis using time of flight detection may be carried out on an Applied Biosystems Bio-Ion 20 mass spectrometer. Electrospray mass spectroscopy may be carried and on a VG-Trio machine. Peptide compounds useful in the invention may also be prepared using recombinant DNA techniques, using methods now known in the art. Saa, e.g., Sambrook et l ^ , Molecular Cloning: A Laboratory Manual, 2d Ed. , Cold Spring Harbor (1989) . Non-peptide compounds useful in the present invention may be prepared by art-known methods. The compounds referenced above may form salts with various inorganic and organic acids and bases. Such salts include salts prepared with organic and inorganic acids, for example, HCl , HBr, H 2 S0 4 , H 3 P0 4 , trifluoroacetic acid, acetic acid, formic acid, methanesulfonic acid, toluenesulfonic acid, maleic acid, fumaric acid and camphorsulfonic acid. Salts prepared with bases include ammonium salts, alkali metal salts, e.g., sodium and potassium salts, and alkali earth salts, e_x≡μ_, calcium and magnesium salts. Acetate, hydrochloride, and trifluoroacetate salts are preferred. The salts may be formed by conventional means, as by reacting the free acid or base forms of the product with one or more equivalents of the appropriate base or acid in a solvent or medium in which the salt is insoluble, or in a solvent such as water which is then removed in vacuo or by freeze-drying or by exchanging the ions of an existing salt for another ion on a suitable ion exchange resin. Formulation and Administration Compounds of the invention are useful in view of their exendin-like effects, and may conveniently be provided in the form of formulations suitable for parenteral (including intravenous, intramuscular and subcutaneous) or nasal or oral administration. In some cases, it will be convenient to provide an exendin or exendin agonist and another anti- gastric-emptying agent, such as glucagon, an amylin, or an amylin agonist, in a single composition or solution for administration together. In other cases, it may be more advantageous to administer another anti-emptying agent separately from said exendin or exendin agonist. In yet other cases, it may be beneficial to provide an exendin or an exendin agonist either co-formulated or separately with other glucose lowering agents such as insulin. A suitable administration format may best be determined by a medical practitioner for each patient individually. Suitable pharmaceutically acceptable carriers and their formulation are described in standard formulation treatises, e.g., Remington's Pharmaceutical Sciences by E.W. Martin. Sa also Wang, Y.J. and Hanson, M.A. "Parenteral Formulations of Proteins and Peptides: Stability and Stabilizers," Journal of Parenteral Science and Technology, Technical Report No. 10, Supp. 42:2S (1988) . Compounds useful in the invention can be provided as parenteral compositions for injection or infusion. They can, for example, be suspended in an inert oil, suitably a vegetable oil such as sesame, peanut, olive oil, or other acceptable carrier. Preferably, they are suspended in an aqueous carrier, for example, in an isotonic buffer solution at a pH of about 5.6 to 7.4. These compositions may be sterilized by conventional sterilization techniques, or may be sterile filtered. The compositions may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions, such as pH buffering agents. Useful buffers include for example, sodium acetate/acetic acid buffers. A form of repository or "depot" slow release preparation may be used so that therapeutically effective amounts of the preparation are delivered into the bloodstream over many hours or days following transdermal injection or delivery. The desired isotonicity may be accomplished using sodium chloride or other pharmaceutically acceptable agents such as dextrose, boric acid, sodium tartrate, propylene glycol , polyols (such as mannitol and sorbitol) , or other inorganic or organic solutes. Sodium chloride is preferred particularly for buffers containing sodium ions. The claimed compounds can also be formulated as pharmaceutically acceptable salts (e.g., acid addition salts) and/or complexes thereof. Pharmaceutically acceptable salts are non-toxic salts at the concentration at which they are administered. The preparation of such salts can facilitate the pharmacological use by altering the physical -chemical characteristics of the composition without preventing the composition from exerting its physiological effect. Examples of useful alterations in physical properties include lowering the melting point to facilitate transmucosal administration and increasing the solubility to facilitate the administration of higher concentrations of the drug. Pharmaceutically acceptable salts include acid addition salts such as those containing sulfate, hydrochloride, phosphate, sulfamate, acetate, citrate, lactate, tartrate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate, eyelohexylsulfamate and quinate. Pharmaceutically acceptable salts can be obtained from acids such as hydrochloric acid, sulfuric acid, phosphoric acid, sulfamic acid, acetic acid, citric acid, lactic acid, tartaric acid, malonic acid, methanesulfonic acid, ethane- sulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, cyclohexylsulfamic acid, and quinic acid. Such salts may be prepared by, for example, reacting the free acid or base forms of the product with one or more equivalents of the appropriate base or acid in a solvent or medium in which the salt is insoluble, or in a solvent such as water which is then removed in vacuo or by freeze-drying or by exchanging the ions of an existing salt for another ion on a suitable ion exchange resin. Carriers or excipients can also be used to facilitate administration of the compound. Examples of carriers and excipients include calcium carbonate, calcium phosphate, various sugars such as lactose, glucose, or sucrose, or types of starch, cellulose derivatives, gelatin, vegetable oils, polyethylene glycols and physiologically compatible solvents. The compositions or pharmaceutical composition can be administered by different routes including intravenously, intraperitoneal , subcutaneous, and intramuscular, orally, topically, or transmucosally . If desired, solutions of the above compositions may be thickened with a thickening agent such as methyl cellulose. They may be prepared in emulsified form, either water in oil or oil in water. Any of a wide variety of pharmaceutically acceptable emulsifying agents may be employed including, for example, acacia powder, a non- ionic surfactant (such as a Tween) , or an ionic surfactant (such as alkali polyether alcohol sulfates or sulfonates, e.g., a Triton) . Compositions useful in the invention are prepared by mixing the ingredients following generally accepted procedures. For example, the selected components may be simply mixed in a blender or other standard device to produce a concentrated mixture which may then be adjusted to the final concentration and viscosity by the addition of water or thickening agent and possibly a buffer to control pH or an additional solute to control tonicity. For use by the physician, the compounds will be provided in dosage unit form containing an amount of an exendin agonist, with or without another anti-emptying agent. Therapeutically effective amounts of an exendin agonist for use in the control of gastric emptying and in conditions in which gastric emptying is beneficially slowed or regulated are those that decrease post-prandial blood glucose levels, preferably to no more than about 8 or 9 mM or such that blood glucose levels are reduced as desired. In diabetic or glucose intolerant individuals, plasma glucose levels are higher than in normal individuals. In such individuals, beneficial reduction or "smoothing" of post-prandial blood glucose levels, may be obtained. As will be recognized by those in the field, an effective amount of therapeutic agent will vary with many factors including the age and weight of the patient, the patient's physical condition, the blood sugar level or level of inhibition of gastric emptying to be obtained, and other factors. Such pharmaceutical compositions are useful in causing gastric hypomotility in a subject and may be used as well in other disorders where gastric motility is beneficially reduced. The effective daily anti-emptying dose of the compounds will typically be in the range of 0.01 or 0.03 to about 5 mg/day, preferably about 0.01 or 0.5 to 2 mg/day and more preferably about 0.01 or 0.1 to 1 mg/day, for a 70 kg patient, administered in a single or divided doses. The exact dose to be administered is determined by the attending clinician and is dependent upon where the particular compound lies within the above quoted range, as well as upon the age, weight and condition of the individual . Administration should begin at the first sign of symptoms or shortly after diagnosis of diabetes mellitus. Administration may be by injection, preferably subcutaneous or intramuscular. Orally active compounds may be taken orally, however dosages should be increased 5-10 fold. Generally, in treating or preventing elevated, inappropriate, or undesired post -prandial blood glucose levels, the compounds of this invention may be administered to patients in need of such treatment in a dosage ranges similar to those given above, however, the compounds are administered more frequently, for example, one, two, or three times a day. The optimal formulation and mode of administration of compounds of the present application to a patient depend on factors known in the art such as the particular disease or disorder, the desired effect, and the type of patient. While the compounds will typically be used to treat human patients, they may also be used to treat similar or identical diseases in other vertebrates such as other primates, farm animals such as swine, cattle and poultry, and sports animals and pets such as horses, dogs and cats. To assist in understanding the present invention the following Examples are included which describe the results of a series of experiments. The experiments relating to this invention should not, of course, be construed as specifically limiting the invention and such variations of the invention, now known or later developed, which would be within the purview of one skilled in the art are considered to fall within the scope of the invention as described herein and hereinafter claimed. EXAMPLE 1 Preparation of amidated peptide hav ng SEQ. ID. NO. [5] The above-identified peptide was assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.) . In general, single-coupling cycles were used throughout the synthesis and Fast Moc (HBTU activation) chemistry was employed. However, at some positions coupling was less efficient than expected and double couplings were required. In particular, residues Asp 9 , Thr 7 and Phe 6 all required double coupling. Deprotection (Fmoc group removal) of the growing peptide chain using piperidine was not always efficient. Double deprotection was required at positions Arg 20 , Val 19 and Leu 14 . Final deprotection of the completed peptide resin was achieved using a mixture of triethylsilane (0.2 mL) , ethanedithiol (0.2 mL) , anisole (0.2 mL) , water (0.2 mL) and trifluoroacetic acid (15 mL) according to standard methods (Introduction to Cleavage Techniques, Applied Biosystems, Inc.) The peptide was precipitated in ether/water (50 mL) and centrifuged. The precipitate was reconstituted in glacial acetic acid and lyophilized. The lyophilized peptide was dissolved in water) . Crude purity was about 55%. Used in purification steps and analysis were Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . The solution containing peptide was applied to a preparative C-18 column and purified (10% to 40% Solvent B in Solvent A over 40 minutes) . Purity of fractions was determined isocratically using a C-18 analytical column. Pure fractions were pooled furnishing the above-identified peptide. Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide gave product peptide having an observed retention time of 14.5 minutes. Electrospray Mass Spectrometry (M) : calculated 4131.7; found 4129.3. EXAMPLE ?. Preparation of Peptide having SEQ. ID. NO. [6] The above-identified peptide was assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis were Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 25% to 75% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide gave product peptide having an observed retention time of 21.5 minutes. Electrospray Mass Spectrometry (M) : calculated 4168.6; found 4171.2. EXAMPLE 3 Preparation of Peptide having SEQ. ID. NO. [7] The above-identified peptide was assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis were Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide gave product peptide having an observed retention time of 17.9 minutes. Electrospray Mass Spectrometry (M) : calculated 4147.6; found 4150.2. EXAMPLE 4 Preparation of Peptide having SEQ. ID. NO. [ 1 The above-identified peptide was assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis were Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 35% to 65% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide gave product peptide having an observed retention time of 19.7 minutes. Electrospray Mass Spectrometry (M) : calculated 4212.6; found 4213.2. Preparation of Peptide having SEQ. ID. NO. [9] The above- identified peptide was assembled on 4-(2'-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis were Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 50% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide gave product peptide having an observed retention time of 16.3 minutes. Electrospray Mass Spectrometry (M) : calculated 4262.7; found 4262.4. EAMPLE__6 Preparation of Peptid Jia3zlng_SEO ^- -IJ-L._M0-. Llfil The above- identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4172.6 EXAMPLE 7 Preparation of Peptide having SEQ. ID. NO. [11] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4224.7. EXAMP E _E Preparation of Peptide having SEQ. ID. NO. [121 The above- identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4172.6 EXAMPLE 9 Preparation of Peptide having SEQ. ID. NO. [13] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4186.6 El Preparation of Peptide having SEQ. ID. NO. [141 The above-identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4200.7 EXAMPLE 11 Preparation of Peptide having SEQ. ID. NO. [15] The above- identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4200.7 Preparation of Peptide having SEQ. ID. NO. [16] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4202.7. EXAMPLE 13 Preparation of Peptide having SEP. ID. NO. [17] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4145.6. EXAMPLE 14 Preparation of Peptide having_ELEQ ^ ID. NO. [18] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4184.6. EXAMPLE 15 Preparation of Peptide having SEQ. ID. NO. [19] The above-identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4145.6. EXAMPLE 16 Preparation of Peptide having SEQ. ID. NO. [20] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4224.7. Preparation of Peptide having SEQ. ID. NO. [21] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4172.6. EXAMPLE 18 Preparation of Peptide having SEQ. ID. NO. [221 The above- identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4115.5. EXAMPLE 19 Preparation of Peptide having SEQ. ID. NO. [23] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4188.6. EXAMPLE 20 Preparation of Peptide having SEP. TD. NO. [241 The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4131.6. Preparation of Peptide having SEQ. ID. NO. [25] The above-identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4172.6. EXAMPLE ■ ?. ■ ?. Preparation of Peptide_having SEQ. ID. NO. [26] The above-identified peptide is assembled on 4- (2 '-4 dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4145.6. Preparation of Peptide having SEQ. ID. NO. f27] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the thioproline positions 38, 37, 36 and 31. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4266.8. EXAMPLE 24 Preparation of Peptide having SEQ. ID. NO. [28] The above- identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the thioproline positions 38, 37 and 36. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4246.8. EXAMPLE 25 Preparation of Peptide having SEQ. ID. NO. [29] The above-identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the homoproline positions 38, 37, 36 and 31. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4250.8. EXAMPLE 26 Preparation of Peptide having SEQ. ID. NO. [30] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the homoproline positions 38, 37, and 36. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4234.8. Preparation of Peptide having SEQ. ID. NO. [31] The above-identified peptide is assembled on 4-(2'-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the thioproline positions 38, 37, 36 and 31. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4209.8. EXAMPLE 28 Preparation of Peptide having SEQ. ID. NO. [32] The above- identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the homoproline positions 38, 37, 36 and 31. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4193.7. Preparation of Peptide having SEQ. ID. NO. [33] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the N- methylalanine positions 38, 37, 36 and 31. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 3858.2. E3 Preparation of Peptide having SEQ. ID. NO. f34] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the N- methylalanine positions 38, 37 and 36. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 3940.3. Preparation of Peptide having SEQ. ID. NO. [35] The above-identified peptide is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the N- methylalanine positions 38, 37, 36 and 31. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 3801.1. EXAMPLE 32 4-Imidazolylpropionyl-Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gin Met Glu Glu Glu Ala Val Arg Leu Phe lie Glu Trp Leu Lys-NH e octanoyl Asn Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser -NH 2 [SEQ. ID. NO. 36] is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the proline positions 38, 37, 36 and 31. Fmoc-Lys-NH e octanoyl acid is used for coupling at position 27. Instead of using protected His for the final coupling at position 1, 4- imidazolylpropionic acid is coupled directly to the N- terminus of residues 2-39 on the resin. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4294.5. EXAMPLE 33 Preparation of Peptide having SEQ. ID. NO. [37] 4-Imidazolylpropionyl-Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gin Leu Glu Glu Glu Ala Val Arg Leu Phe lie Glu Phe Leu Lys-NH e octanoyl Asn Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser-NH 2 [SEQ. ID. NO. 37] is assembled on 4- (2 '-4'- dimethoxyphenyl) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the proline positions 38, 37, 36 and 31. Fmoc-Lys-NH e octanoyl acid is used for coupling at position 27. Instead of using protected His for the final coupling at position 1, 4- imidazolylpropionic acid is coupled directly to the N- terminus of residues 2-39 on the resin. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4242.7. EXAMPLE 34 Preparat on of Peptide having SEQ. ID. NO. r38] 4-Imidazolylpropionyl-Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gin Met Glu Glu Glu Ala Val Arg Leu Phe He Glu Trp Leu Asn Lys-NH e octanoyl Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser-NH 2 [SEQ. ID. NO. 38] is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the proline positions 38, 37, 36 and 31. Fmoc-Lys-NH e octanoyl acid is used for coupling at position 28. Instead of using protected His for the final coupling at position 1, 4- imidazolylpropionic acid is coupled directly to the N- terminus of protected residues 2-39 on the resin. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4294.5. EXAMPTjE_3_5 Preparation of Peptide having SEQ. ID. NO. [39] 4-Imidazolylpropionyl-Gly Glu Gly Thr Phe Thr Ser Asp Leu Ser Lys Gin Leu Glu Glu Glu Ala Val Arg Leu Phe He Glu Phe Leu Asn Lys-NH e octanoyl Gly Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser -NH 2 [SEQ. ID. NO. 39] is assembled on 4- (2 '-4'- dimethoxyphenyl ) -Fmoc aminomethyl phenoxy acetamide norleucine MBHA resin (Novabiochem, 0.55 mmole/g) using Fmoc- protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Additional double couplings are required at the proline positions 38, 37, 36 and 31. Fmoc-Lys-NH e octanoyl acid is used for coupling at position 28. Instead of using protected His for the final coupling at position 1, 4- imidazolylpropionic acid is coupled directly to the N- terminus of residues 2-39 on the resin. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN) . Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry (M) : calculated 4242.7. Preparation of C-terminal carboxylic acid Peptidescorresponding to the above C-terminal amide The above peptides of Examples 1 to 35 are assembled on the so called Wang resin (p-alkoxybenzylalacohol resin (Bachem, 0.54 mmole/g)) using Fmoc-protected amino acids (Applied Biosystems, Inc.), cleaved from the resin, deprotected and purified in a similar way to Example 1. Used in analysis are Solvent A (0.1% TFA in water) and Solvent B (0.1% TFA in ACN). Analytical RP-HPLC (gradient 30% to 60% Solvent B in Solvent A over 30 minutes) of the lyophilized peptide is then carried out to determine the retention time of the product peptide. Electrospray Mass Spectrometry provides an experimentally determined (M) . EXAMPLES A TO D Reagents Used GLP-1 was purchased from Bachem (Torrance, CA) , all other peptides were prepared in house using synthesis methods such as those described therein. All chemicals were of the highest commercial grade. The cAMP SPA immunoassay was purchased from Amersham. The radioligands were purchased from New England Nuclear (Boston, MA) . RINm5f cells (American Type Tissue Collection, Rockville, MD) were grown in DME/F12 medium containing 10% fetal bovine serum and 2mM L-glutamine. Cells were grown at 37°C and 5% C0 2 /95% humidified air and medium was replaced every 2 to 3 days. Cells were grown to confluence then harvested and homogenized using on a Polytron homogenizer. Cell homogenates were stored frozen at -70°C until used. GLP-1 Receptor Binding Studies Receptor binding was assessed by measuring displacement of [ 125 I] human GLP-1 (7-36) or [ 125 I] Exendin (9-39) from RINm5f membranes. Assay buffer contained 5 μg/ml bestatin 1 μg.ml phosphoramidon, 1 mg/ml bovine serum albumin (fraction V), 1 mg/ml bacitracin, and 1 mM MgCl 2 in 20 mM HEPES, pH 7.4 To measure binding, 30 μg membrane protein (Bradford protein assay) was resuspended in 200 μl assay buffer and incubated with 60 pM [ 125 I] human GLP-1 or Exendin (9-39) and unlabeled peptides for 120 minutes as 23°C in 96 well plates (Nagle Nunc, Rochester, NY) . Incubations were terminated by rapid filtration with cold phosphatebuffered saline, pH 7.4, through polyethyleneimine-treated GF/B glass fiber filters (Wallac Inc., Gaithersburg, MD) using a Tomtec Mach II plate harvester (Wallac Inc., Gaithersburg, MD) . Filters were dried, combined with scintillant, and radioactivity determined in a Betaplate liquid scintillant counter (Wallac Inc . ) . Peptide samples were run in the assay as duplicate points at 6 dilutions over a concentration range of 10 "6 M to 10 ~12 M to generate response curves. The biological activity of a sample is expressed as an IC 50 value, calculated from the raw data using an iterative curve- fitting program using a 4- parameter logistic equation (Prism, GraphPAD Software) . Example B Cyclase Activation Study Assay buffer contained 10 μM GTP, 0.75 mM ATP, 2.5 mM MgCl 2 , 0.5mM phosphocreatine, 12,5 U/ml creatine kinase, 0.4 mg/ml aprotinin, 1 μM IBMX in 50 mM HEPES, pH 7.4. Membranes and peptides were combined in 100 ml of assay buffer in 96 well filter-bottom plates (Millipore Corp., Bedford, MA). After 20 minutes incubation at 37°C, the assay was terminated by transfer of supernatant by filtration into a fresh 96 well plate using a Millipore vacuum manifold. Supernatant cAMP contents were quantitated by SPA immunoassay. Peptide samples were run in the assay as triplicate points at 7 dilutions over a concentration range of 10 ~6 M to 10 ~1 M to generate response curves. The biological activity of a particular sample was expressed as an EC 50 value, calculated as described above. Results are tabulated in Table I . ΪΔBLE 1 Activity in the RINm5f cyclase assay EC, Exendin-4 [SEQ. ID. NO. 2] 0.23 Compound 1 [SEQ. ID. NO. 5] 0.17 Compound 2 [SEQ. ID. NO. 6] 0.23 Compound 3 [SEQ. ID. NO. 7] 0.42 Example C Determination of Blood Glucose Levels in db/db Mice - 1 Hour C57BL/6J-m=/=Lepr db mice, at least 3 months of age were utilized for the study. The mice were obtained from The Jackson Laboratory and allowed to acclimate for at least one week in the vivarium. Mice were housed in groups of ten at 22° +1°C with a 12:12 light :dark cycle, with lights on at 6 a.m. All animals were deprived of food for 2 hours before taking baseline blood samples. Approximately 100 μl of blood was drawn from each mouse via eye puncture, after a light anesthesia with metophane . After collecting baseline blood samples, to measure plasma glucose concentrations, all animals receive subcutaneous injections of either vehicle, exendin-4 or test compound in concentrations indicated. Blood samples were drawn again, using the same procedure, after exactly one hour from the injections, and plasma glucose concentrations were measured. For each animal, the % change in plasma value, from baseline value, was calculated and a dose dependent relationship was evaluated using Graphpad Prizm™ software. Figure 5 depicts the effects of varying doses of exendin-4 and Compound 1 of Figure 1 [SEQ. ID. NO. 5] on plasma glucose levels. Example D The following study was carried out to examine the effects of exendin-4, exendin-4 acid and an exendin agonist (Compound 1 of Figure 1 [SEQ. ID. NO. 5]) on gastric emptying in rats. This experiment followed a modification of the method of Scarpignato, et al . , Arch. Int. Pharmacodyn. Ther. 246:286-94 (1980) . Male Harlan Sprague Dawley (HSD) rats were used. All animals were housed at 22.7+0.8 C in a 12:12 hour light: dark cycle (experiments being performed during the light cycle) and were fed and watered ad libi tum (Diet LM-485, Teklad, Madison, WI) . Exendin-4 and exendin-4 acid were synthesized according to standard peptide synthesis methods. The preparation of Compound 1 [SEQ. ID. NO. 5] is described in Example 1. The determination of gastric emptying by the method described below was performed after a fast of -20 hours to ensure that the stomach contained no chyme that would interfere with spectrophotometric absorbance measurements. Conscious rats received by gavage, 1.5ml of an acaloric gel containing 1.5% methyl cellulose (M-0262, Sigma Chemical Co, St Louis, MO) and 0.05% phenol red indicator. Twenty minutes after gavage, rats were anesthetized using 5% halothane, the stomach exposed and clamped at the pyloric and lower esophageal sphincters using artery forceps, removed and opened into an alkaline solution which was made up to a fixed volume. Stomach content was derived from the intensity of the phenol red in the alkaline solution, measured by absorbance at a wavelength of 560 nm. In separate experiments on 7 rats, the stomach and small intestine were both excised and opened into an alkaline solution. The quantity of phenol red that could be recovered from the upper gastrointestinal tract within 20 minutes of gavage was 89+4%; dye which appeared to bind irrecoverably to the gut luminal surface may have accounted for the balance. To account for a maximal dye recovery of less than 100%, percent of stomach contents remaining after 20 min were expressed as a fraction of the gastric contents recovered from control rats sacrificed immediately after gavage in the same experiment. Percent gastric contents remaining = (absorbance at 20 min) / (absorbance at 0 mm) x 100. In baseline studies, with no drug treatment, gastric emptying over 20 min was determined. In dose-response studies, rats were treated with 0.01, 0.1, 0.3, 1, 10 and 100 μg of exendin-4, 0.01, 0.03, 0.1, 1, 10 and 100 μg exendin-4 acid, and 0.1, 0.3, 1, 10 and 100 μg of Compound 1 [SEQ. ID. NO. 5] . The results are shown in Figure 6. The results, shown in Figure 6 and Table II, show that the exendin agonists, exendin-4 acid and compound 1 are potent inhibitors of gastric emptying. The EC 50 of exendin-4 was 0.27 μg. The μg and 0.29 μg, respectively) . TABLE II EC S0 _(μgl exendin-4 0.27 exendin-4 acid 0.12 Conpound 1 0.29 50

Claims: We claim: 1. A peptide compound of the formula (I) [SEQ. ID. NO. 4] : 1 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa 4 Xaa 5 Xaa 6 Xaa 7 Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa lx Xaa 12 Xaa 13 Leu Lys Asn Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa x is His, Arg or Tyr; Xaa 2 is Ser, Gly, Ala or Thr; Xaa 3 is Asp or Glu; Xaa 4 is Phe, Tyr or naphthylalanine; Xaa 5 is Thr or Ser Xaa 6 is Ser or Thr Xaa, is Asp or Glu; Xaa 8 is Leu, He, Val, pentylglycine or Met; Xaa 9 is Leu, He, pentylglycine, Val or Met; Xaa 10 is Phe, Tyr or naphthylalanine; Xaa n is He, Val, Leu, pentylglycine, tert-butylglycine or Met; Xaa 12 is Glu or Asp; Xaa 13 is Trp, Phe, Tyr, or naphthylalanine; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently Pro, homoproline, 3Hyp, 4Hyp, thioproline, N- alkylglycine, N-alkylpentylglycine or N- alkylalanine ; Xaa 18 is Ser, Thr or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. ID. NOS. 1 or 2; and pharmaceutically acceptable salts thereof. 2. A compound according to claim 1 wherein Xaa ! is His or Tyr . 3. A compound according to claim 2 wherein Xaa x is His. 4. A compound according to claim 2 wherein Xaa 2 is Gly. 5. A compound according to claim 4 wherein Xaa 9 is Leu, pentylglycine or Met. 6. A compound according to claim 5 wherein Xaa 13 is Trp or Phe . 7. A compound according to claim 6 wherein Xaa 4 is Phe or naphthylalanine; Xaa 10 is Phe or naphthylalanine; Xaa n is He or Val and Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N- alkylalanine . 8. A compound according to claim 7 wherein Xaa 18 is Ser or Tyr . 9. A compound according to claim 8 wherein Xaa 18 is Ser. 10. A compound according to claim 9 wherein Z is -NH 2 . 11. A compound according to claim 1 wherein Xaa 2 is Gly. 12. A compound according to claim 1 wherein Xaa 9 is Leu, pentylglycine or Met . 13. A compound according to claim 1 wherein Xaa 13 is Trp or Phe . 14. A compound according to claim 1 wherein Xaa 4 is Phe or naphthylalanine; Xaa 10 is Phe or naphthylalanine; Xaa lx is He or Val and Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N- alkylalanine . 15. A compound according to claim 1 wherein Xaa 18 is Ser or Tyr . 16. A compound according to claim 1 wherein Z is -NH 2 . 17. A compound according to claim 1 which has an amino acid sequence selected from SEQ. ID. NOS. 5 to 35. 18. A peptide compound of the formula (I) [SEQ. ID. NO. 4] 1 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa 4 Xaa 5 Xaa 6 Xaa 7 Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa xl Xaa 12 Xaa 13 Leu Lys Asn Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa ! is His or Arg; Xaa 2 is Gly or Ala; Xaa 3 is Asp or Glu; Xaa 4 is Phe or naphthylalanine; Xaa 5 is Thr or Ser Xaa 6 is Ser or Thr Xaa, is Asp or Glu; Xaa 8 is Leu or pentylglycine; Xaa 9 is Leu or pentylglycine; Xaa 10 is Phe or naphthylalanine; Xaa xl is He, Val or tert-butylglycine; Xaa 12 is Glu or Asp; Xaa 13 is Trp or Phe; Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently Pro, homoproline, thioproline or N-methylalanine ; Xaa 18 is Ser or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. ID. NOS. 1 or 2; and pharmaceutically acceptable salts thereof. 19. A compound according to claim 18 which has an amino acid sequence selected from SEQ. ID. NOS. 5, 6, 17, 18, 19, 22, 24, 31, 32 and 35. 20. A compound according to claim 18 which is exendin-3 acid. 21. A compound according to claim 18 which is exendin-4 acid. 22. A composition comprising a compound of any of claims 1-18, 20 or 21 in a pharmaceutically acceptable carrier. 23. A composition comprising a compound of claim 19 in a pharmaceutically acceptable carrier. 24. A peptide compound of the formula (II) [SEQ. ID. NO. 40] : 5 10 Xaa- L Xaa 2 Xaa 3 Gly Thr Xaa 4 Xaa 5 Xaa 6 Xaa, Xaa 8 15 20 Ser Lys Gin Xaa 9 Glu Glu Glu Ala Val Arg Leu 25 30 Xaa 10 Xaa lx Xaa 12 Xaa 13 Leu X λ Gly Gly Xaa 14 35 Ser Ser Gly Ala Xaa 15 Xaa 16 Xaa 17 Xaa 18 -Z wherein Xaa x is His, Arg, Tyr or 4-imidazopropionyl ; Xaa 2 is Ser, Gly, Ala or Thr; Xaa 3 is Asp or Glu; Xaa 4 is Phe, Tyr or naphthylalanine; Xaa 5 is Thr or Ser Xaa 6 is Ser or Thr Xaa, is Asp or Glu; Xaa 8 is Leu, He, Val, pentylglycine or Met; Xaa 9 is Leu, He, pentylglycine, Val or Met; Xaa 10 is Phe, Tyr or naphthylalanine; Xaa xl is He, Val, Leu, pentylglycine, tert-butylglycine or Met; Xaa 12 is Glu or Asp; Xaa 13 is Trp, Phe, Tyr, or naphthylalanine; X- L is Lys Asn, Asn Lys, Lys-NH e -R Asn, Asn Lys-NH e -R where R is Lys, Arg, C^C-^ straight chain or branched alkanoyl or cycloalkylalkanoyl Xaa 14 , Xaa 1? , Xaa 16 and Xaa 17 are independently Pro, homoproline, 3Hyp, 4Hyp, thioproline, N-alkylglycine, N-alkylpentylglycine or N-alkylalanine; Xaa 18 is Ser, Thr or Tyr; and Z is -OH or -NH 2 ; with the proviso that the compound does not have the formula of either SEQ. ID. NOS. 1 or 2; and pharmaceutically acceptable salts thereof. 25. A compound according to claim 24 wherein Xaa x is His, Tyr or 4-imidazopropionyl . 26. A compound according to claim 25 wherein Xaa x is His or 4-imidazopropionyl . 27. A compound according to claim 24 wherein Xaa 2 is Gly. 28. A compound according to claim 24 wherein Xaa 9 is Leu, pentylglycine or Met. 29. A compound according to claim 24 wherein Xaa 13 is Trp or Phe . 30. A compound according to claim 24 wherein X λ is Lys Asn, or Lys-NH e -R Asn, where R is Lys, Arg, C^C^ straight chain or branched alkanoyl . 31. A compound according to claim 24 wherein Xaa 4 is Phe or naphthylalanine; Xaa 10 is Phe or naphthylalanine; Xaa ι:L is He or Val and Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N- alkylalanine . 32. A compound according to claim 24 wherein Xaa 18 is Ser or Tyr. 33. A compound according to claim 32 wherein Xaa 18 is Ser. 34. A compound according to claim 24 wherein Z is -NH 2 . 35. A compound according to claim 24 wherein Xaa 4 is Phe or naphthylalanine; Xaa 10 is Phe or naphthylalanine; Xaa xl is He or Val, λ is Lys Asn, or Lys-NH e -R Asn, where R is Lys, Arg, C- L -C- LO straight chain or branched alkanoyl and Xaa 14 , Xaa 15 , Xaa 16 and Xaa 17 are independently selected from Pro, homoproline, thioproline or N-alkylalanine. 36. A compound according to claim 35 wherein Xaa 18 is Ser or Tyr. 37. A compound according to claim 35 wherein Z is -NH 2 . 38. A compound according to claim 22 which has an amino acid sequence selected from SEQ. ID. NOS. 36-39. 39. A composition comprising a compound of any of claims 24-37 in a pharmaceutically acceptable carrier. 40. A composition comprising a compound of claim 38 in a pharmaceutically acceptable carrier.

Inventors: BEELEY NIGEL ROBERT ARNOLD, PRICKETT KATHRYN S, BEELEY, NIGEL, ROBERT, ARNOLD, PRICKETT, KATHRYN, S.
Applicants: AMYLIN PHARMACEUTICALS INC, BEELEY NIGEL ROBERT ARNOLD, PRICKETT KATHRYN S, AMYLIN PHARMACEUTICALS, INC., BEELEY, NIGEL, ROBERT, ARNOLD, PRICKETT, KATHRYN, S.
Application year: 1998
Main classification: A61K38
IPC codes: A61K38, A61P3, C07K14