LOW-VOLTAGE ACTIVATED CALCIUM CHANNEL COMPOSITIONS AND METHODS

Abstract: Isolated nucleic acid encoding low voltage activated calcium channel subunits, including subunits encoded by nucleic acid that arises as splice variants of primary transcripts, is provided. Cells and vectors containing the nucleic acid and methods for identifying compounds that modulate the activity of calcium channels that contain these subunits are also provided.

Description: LOW-VOLTAGE ACTIVATED CALCIUM CHANNEL COMPOSITIONS AND METHODS RELATED APPLICATIONS Benefit of priority to U.S. application Serial No. 08/984,709, to Williams et al. , entitled, "CALCIUM CHANNEL COMPOSITIONS AND METHODS" filed December 3, 1 997, and to U.S. application Serial No. 09/1 88,932, to Williams et al. , entitled, "CALCIUM CHANNEL COMPOSITIONS AND METHODS" filed November 10, 1 998 is claimed herein. This application is related to U.S. application Serial No. 08/450,272, filed May 25, 1 995, U.S. application Serial No. 08/450,273, filed May 25, 1 995, U.S. application Serial No. 08/450,562, filed May 25, 1 995. Each of these applications is a continuation-in-part of U.S. application Serial No. 08/290,01 2. This application is also related to International PCT application No. PCT/US94/09230, filed August 1 1 , 1 994, which claims priority to U.S. application Serial Nos. 08/105,536 and 08/149,097. This application is also related to U.S. application Serial No. 08/404,354, filed February 1 5, 1995, now U.S. Patent No. 5,61 8,720, which is a continuation of U.S. application Serial No. 07/914,231 , filed July 1 3, 1 992, now U.S. Patent No. 5,407,820, and also U.S. application Serial No. 08/314,083, filed September 28, 1994, now U.S. Patent No. 5,686,241 , U.S. application Serial No. 08/435,675, filed May 5, 1 995, now U.S. Patent No. 5,710,250, each of which is a divisional of U.S. application Serial No. 07/914,231 . U.S. application Serial No. 07/914,231 is a continuation of U.S. application Serial No. 07/603,751 , filed November 8, 1 990, now abandoned, which is the national stage of International PCT Application PCT/US89/01408, filed April 4, 1 989, which is a continuation-in-part of U.S. application Serial No. 07/1 76,899, filed April 4, 1 988, now abandoned. This application is also related to U.S. application Serial No. 08/884,599, filed June 27, 1 997, which is a continuation of U.S. application Serial No. 08/314,083. This application is also related to U.S. application Serial No. 08/290,01 2, filed August 1 1 , 1 994, now abandoned, which corresponds to published International PCT application No. WO95/04822, which is a continuation-in-part of allowed U.S. application Serial No. 08/149,097, filed November 5, 1 993, and a continuation-in-part of United States Application Serial No. 08/105,536, filed August 1 1 , 1 993. United States Application Serial No. 08/149,097 is a continuation-in-part of United States Application Serial No. 08/105,536, which is a continuation-in-part of the above-mentioned United States Application Serial No. 07/603,751 , filed November 8, 1 990. This application is also a related to allowed U.S. application Serial No. 08/223,305, filed April 4, 1 994, now U.S. Patent No. 5,851 ,824, which is a continuation of U.S. application Serial No. 07/868,354, now abandoned, which is a continuation-in-part of U.S. application Serial No. 07/745,206, filed August 1 5, 1 991 , now U.S. Patent No. 5,429,921 , which is a continuation-in-part of the above-mentioned United States Application Serial No. 07/603,751 , filed November 8, 1 990, and a continuation-in-part of U.S. application Serial No. 07/620,250, filed November 30, 1 990, now abandoned . This application is also related to allowed application U.S. application Serial No. 08/455,543, filed May 31 , 1 995, now U.S. Patent No. 5,792,846, which is a continuation of U.S. application Serial No. 07/868,354, filed April 10, 1 992. This application is also a related to U.S. application Serial No. 08/31 1 ,363, filed September 23, 1 994, which is a continuation of allowed U.S. application Serial No. 07/745,206, filed August 1 5, 1 991 . This application is also related to allowed U.S. application Serial No. 08/1 93,078, now U.S. Patent No. 5,846,756, filed February 7, 1 994, which is the National Stage of International PCT Application No. PCT/US92/06903, published as International PCT application No. WO93/04083, filed August 1 4, 1 992 and which is a continuation-in-part of U.S. application Serial Nos. 07/868,354, 07/745,206, 07/603,751 , 07/1 76,899, 07/620,250, filed November 30, 1 990, now abandoned, and 07/482,384, now U.S. Patent No. 5,386,025, filed February 2, 1 990. This application is also related to allowed U.S. application Serial No. 08/336,257, now U.S. Patent No. 5,726,035, filed November 7, 1 994, which is a continuation of 07/482,384, now U.S. Patent No. 5,386,025, filed February 2, 1 990. Where permitted, the subject matter of each of the above-noted U.S. applications, patents and International PCT applications is incorporated herein in its entirety. TECHNICAL FIELD The present invention relates to molecular biology and pharmacology. More particularly, the invention relates to calcium channel compositions and methods of making and using the same. BACKGROUND OF THE INVENTION Calcium channels are membrane-spanning, multi-subunit proteins that allow controlled entry of Ca 2 + ions into cells from the extracellular fluid. Cells throughout the animal kingdom, and at least some bacterial, fungal and plant cells, possess one or more types of calcium channel. The most common type of calcium channel is voltage dependent. All "excitable" cells in animals, such as neurons of the central nervous system (CNS), peripheral nerve cells and muscle cells, including those of skeletal muscles, cardiac muscles, and venous and arterial smooth muscles, have voltage-dependent calcium channels (VGCCs) . "Opening" of a voltage-dependent channel to allow an influx of Ca 2 + ions into the cells requires a depolarization to a certain level of the potential difference between the inside of the cell bearing the channel and the extracellular environment bathing the cell. The rate of influx of Ca 2 + into the cell depends on this potential difference. Calcium channels are multisubunit proteins that contain two large subunits, designated a and a 2 , which have molecular weights between about 1 30 and about 200 kilodaltons ("kD"), and one to three different smaller subunits of less than about 60 kD in molecular weight. At least one of the larger subunits and possibly some of the smaller subunits are glycosylated. Some of the subunits are capable of being phosphorylated. The a subunit has a molecular weight of about 1 50 to about 1 70 kD when analyzed by sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) after isolation from mammalian muscle tissue and has specific binding sites for various 1 ,4-dihydropyridines (DHPs) and phenylalkylamines. Under non-reducing conditions (in the presence of N-ethylmaleimide), the a 2 subunit migrates in SDS-PAGE as a band corresponding to a molecular weight of about 1 60-1 90 kD. Upon reduction, a large fragment and smaller fragments are released. The β subunit of the rabbit skeletal muscle calcium channel is a phosphorylated protein that has a molecular weight of 52-65 kD as determined by SDS- PAGE analysis. This subunit is insensitive to reducing conditions. The y subunit of the calcium channel appears to be a glycoprotein with an apparent molecular weight of 30-33 kD, as determined by SDS-PAGE analysis. In order to study calcium channel structure and function, large amounts of pure channel protein are needed. Because of the complex nature of these multisubunit proteins, the varying concentrations of calcium channels in tissue sources of the protein, the presence of mixed populations of calcium channels in tissues, difficulties in obtaining tissues of interest, and the modifications of the native protein that can occur during the isolation procedure, it is extremely difficult to obtain large amounts of highly purified, completely intact calcium channel protein. Because calcium channels are present in various tissues and have a central role in regulating intracellular calcium ion concentrations, they are implicated in a number of vital processes in animals, including neurotransmitter release, muscle contraction, pacemaker activity, and secretion of hormones and other substances. These processes appear to be involved in numerous human disorders, such as central nervous system disorders and cardiovascular diseases. Calcium channels, thus, are also implicated in numerous disorders. A number of compounds useful for treating various cardiovascular diseases in animals, including humans, are thought to exert their beneficial effects by modulating functions of voltage-dependent calcium channels present in cardiac and/or vascular smooth muscle. Many of these compounds bind to calcium channels and block, or reduce the rate of, influx of Ca 2 + into the cells in response to depolarization of the cell membrane. The results of studies of recombinant expression of rabbit calcium channel a subunit-encoding cDNA clones and transcripts of the cDNA clones indicate that the σ, subunit forms the pore through which calcium enters cells. The relevance of the barium currents generated in these recombinant cells to the actual current generated by calcium channels containing as one component the respective a^ subunits in vivo is unclear. In order to completely and accurately characterize and evaluate different calcium channel types, however, it is essential to examine the functional properties of recombinant channels containing all of the subunits as found in vivo. In order to conduct this examination and to fully understand calcium channel structure and function, it is critical to identify and characterize as many calcium channel subunits as possible. Also in order to prepare recombinant cells for use in identifying compounds that interact with calcium channels, it is necessary to be able to produce cells that express uniform populations of calcium channels containing defined subunits. An understanding of the pharmacology of compounds that interact with calcium channels in other organ systems, such as the CNS, may aid in the rational design of compounds that specifically interact with subtypes of human calcium channels to have desired therapeutic effects, such as in the treatment of neurodegenerative and cardiovascular disorders. Such understanding and the ability to rationally design therapeutically effective compounds, however, have been hampered by an inability to independently determine the types of human calcium channels and the molecular nature of individual subtypes, particularly in the CNS, and by the unavailability of pure preparations of specific channel subtypes to use for evaluation of the specificity of calcium channel- effecting compounds. Thus, identification of DNA encoding human calcium channel subunits and the use of such DNA for expression of calcium channel subunits and functional calcium channels would aid in screening and designing therapeutically effective compounds. Multiple types of calcium channels have been identified in mammalian cells from various tissues, including skeletal muscle, cardiac muscle, lung, smooth muscle and brain, (see, e.g. , Bean, B. P. (1 989) Ann. Rev. Physio/. 57 :367-384 and Hess, P. ( 1 990) Ann. Rev. Neurosci. 56:337) . The different types of calcium channels have been broadly categorized into four classes, L-, T-, N-, P-, Q and R-type, distinguished by current kinetics, holding potential sensitivity and sensitivity to calcium channel agonists and antagonists. The primary determinant of diversity among calcium channels is the nature of the pore-forming a subunit. Nucleic acid encoding numerous a subunits has been cloned and the encoded subunits expressed. Correlations between a subunits and the operationally defined Ca 2 + currents have been established. Six gene products a- \ A - a \ - anc ' σ ιs participate in the formation of high-voltage activated channels, which include the L, N, P, Q and R-type channels. DNA encoding human o^-subunits, including σ 1A -, σ 1B -, σ 1 c -, σ 1 D - and σ 1 E subunits and splice variants thereof has been described (see, e.g., U.S. Patent No. 5,429,921 , U.S. Patent No. 5,846,756, U.S. Patent No. 5,851 ,824, published International PCT application No. PCT/US92/06903, and published International PCT application No. PCT/US94/09230) . These subunits appear to participate in formation of high voltage calcium (HVA) channels, which in addition to one of these σ subunits, includes a β subunit and an σ 2 -subunit, including δ, which is linked to a 2 by a disulfide bridge and arises from the same precursor. The distinct biophysical and pharmacological properties of each channel derive primarily form the α^-subunit, but are modulated by the ancillary subunits, principally the β subunits associated with the channel. /?-subunits have been shown to increase the peak current amplitude, to shift activation/inactivation curves toward more hyperpolarized potentials and to alter kinetics of activation and inactivation (see, e.g. , Lambert et a_L (1 997) J. Neurosci. 17:6621 -6625) . The a 2 δ subunit, which is tissue- specific, increases the current generated by any σ, subunit and potentiates the stimulatory response of β subunits. T-type or LVA channels Little is known about the channels that have been designated T- channels or LVA (low voltage activated) channels. Low-voltage activated (LVA), i.e., T-type, calcium channels are reportedly found in a variety of cell types. Low-voltage activated (LVA) or T-type calcium channels are also widely distributed in the central and peripheral nervous system and apparently involved in an extensive array of different neuronal processes. In general it is believed that T-type currents do not differ fundamentally from other Ca 2 + currents. Like HVA channels, T-type channels are selectively permeable to divalent cations, as long as a minimal concentration of divalent cations is present in the external medium. For LVA (or T-type) currents, this minimal Ca 2 + concentration is about 25 μm, and for HVA currents it is about 1 μM. T-type current is reported to saturate with a K d of about 1 0 mM Ca 2 + , which is similar to that reported for HVA currents. The channels, however, appear to exhibit certain differences. They differ in their relative permeability to divalent cations. In general, HVA channels are more permeable to Ba 2 + than to Ca 2 + ; T-type are equally or slightly less permeable to Ba 2 + than to Ca 2 + . T-type channels also are believed to exhibit slower activation/inactivation and deactivation kinetics and have been reported to exhibit relatively higher sensitivity to Ni 2 + . This type of channel is activated near the resting potential of the membrane, and is believed to be responsible for the generation of repetitive firing activity or intrinsic neuronal oscillations and for Ca 2 + entry accompanying the spike activity (see, e.g. , Huguenard ( 1 996) Annual Rev. Physiol. 58:329-348) . Recent data suggests that β- subunits identified to date may not be a constitutive T-type channel subunit (see, Lambert et al. ( 1 997) J . Neurosci. 1 7:6621 -6625) . The structure of calcium channels that generate the various LVA currents is unknown. None of the a subunits previously cloned appear to have all properties that have been ascribed to the low voltage-activated T-type (or LVA) channels. Therefore, it is an object herein, to provide nucleic acid encoding specific calcium channel subunits that have structural and functional properties that differ from the HVA type channels. It is also an object herein to provide nucleic acid encoding channels that have activities that have been ascribed to T-type channels and to provide eukaryotic cells bearing recombinant tissue-specific or subtype-specific calcium channels. It is also an object to provide assays for identification of potentially therapeutic compounds that act as modulators of calcium channel activity, particularly those specific for channels that exhibit properties of human T-type channels and other types of channels. SUMMARY OF THE INVENTION Isolated and purified nucleic acid fragments that encode calcium channel subunits are provided. The subunits form low-voltage activated (LVA) channels, particularly channels that have properties associated with T-type channels. The subunits and results provided herein, provide a family of a subunits corresponding to LVA, or T-type, channels. Channels that contain these subunits have ability to open at low potential difference, but stay open for only moderate time periods. These channels are located in critical physiologic locations, including neurons in the thalamus, hypothalamus, and brain stem, and consequently may be involved in autonomic nervous functions, perhaps involved in regulation of cardiovascular activities such as heart rate, arterial and venous smooth muscle innervation and tone, pulmonary rate and other critical physiologic activities. DNA encoding these a subunits of a animal channels, and RNA, encoding such subunits, made upon transcription of such DNA are provided . In particular, nucleic acid that encodes T-type calcium channels, designated σ 1 H -subunits (designated σ 1 F in the priority document U.S. application Serial No. 08/984,709) of a calcium channel, particularly an animal calcium channel and more particularly a mammalian calcium channel is provided. Of particular interest herein is the nucleic acid that encodes the σ 1 H subunits of calcium channels, particularly mammalian calcium channels. Nucleic acid encoding exemplary σ 1 H subunits are provided. Nucleic acid encoding two splice variants, designated σ 1 h and σ 1 H . 2 , from human calcium channels is provided. The nucleic acid sequences and encoded amino acids of the exemplified subunits are set forth in SEQ ID Nos. 1 2 (σ 1 H .,), 1 5 (σ 1 H .ι) and 1 6 (α 1 H . 2 ) . SEQ ID NOs. 1 2 and 1 5 differ only in that in amino acid 2230 (bases 6983-6985) is Asp (GAC) in the SEQ ID No. 1 5 and Glu (GAA) in SEQ ID No. 1 2. This nucleic acid can be used to isolate variants, including additional splice variants of the nucleic acid encoding 1 H subunits, allelic variants and σ 1 H subunits from other animals, particularly mammals. Such nucleic acid includes DNA encoding an σ 1 hM subunit that has substantially the same sequence of amino acids as encoded by the DNA set forth in SEQ ID Nos. 1 2 and 1 5. This nucleic acid can also be used to isolate DNA encoding σ 1 H subunits from other species, particularly other mammals. Also provided is nucleic acid that encodes a second splice variant, designated σ 1 H . 2 , is provided. The nucleic acid sequence of this variant, differs from a 1 H .., in having a 957 nucleotide deletion, resulting in loss of 31 9 amino acids (corresponding to amino acids 470-788 of α 1 H . ι ) . Also included are any subunits that are encoded by nucleic acid containing nucleotides nt 1 506 to nt 2627 of SEQ ID No. 1 2 or 1 5 or subunits that are encoded by nucleic acid that hybridizes, preferably under conditions of high stringency, to a probe derived from this region and that encodes a T-channel, which can be identified using methods herein. The 1 H subunit differs from the 7 1 A -σ 1 E calcium channel subunits in a number of aspects. First, the intracellular loop positioned between transmembrane Domains I and II is considerably longer than HVA calcium channels. For instance, as exemplified in SEQ ID Nos. 1 2 and 1 5 and described below, the intracellular loop between Domains I and II is greater than 1 , 100 nt (1 1 22 nt), whereas the corresponding region in HVA calcium channels ranges from 351 to 381 nt in length. Thus, the intracellular loop of σ 1 H contains approximately 370 additional amino acid residues (aa 420 to aa 794 of SEQ ID No. 1 2) not found in HVA calcium channel a subunits. In addition, the encoded amino acid sequence of this loop region is highly proline rich and contains a poly-HIS region of 9 consecutive histidine residues. Other distinguishing features of the σ 1 H subunit, include the absence of amino acid residues in the intracellular loop between transmembrane Domains I and II that are known to be critical (e.g. , see De Waard et al. ( 1 996) FEBS Letters 380:272-276; Pragnell et al. ( 1 994) Nature 368:67-70) for the interaction between an α subunit and a β subunit. The σ 1 H subunit also contains a notably large extracellular loop in Domain I between IS5 and IS6. T he HVA σ, calcium channel subunits provided herein contain 249-270 nucleotide residues in this loop. In contrast, the human σ 1 H subunit contains 426 nucleotide residues in this loop. The intracellular loop between transmembrane Domains III and IV is also slightly larger than the HVA a^ subunits (1 86 nt compared to 1 59- 1 65 nt). Nucleic acid probes, which can be labeled for detection, containing at least about 14, preferably 1 6, or, if desired, 20 or 30 or more, contiguous nucleotides of σ 1 H -encoding nucleic acid are provided. Methods using the probes for the isolation and cloning of calcium channel subunit-encoding DNA, including splice variants within tissues and inter- tissue variants are also provided. Particularly preferred regions from which to construct probes for the isolation of DNA encoding a human σ 1 H subunit include the nucleic acid sequence encoding the notably long intracellular loop located between transmembrane Domains I and II (e.g., nt 1 506 to nt 2627 of SEQ ID Nos. 1 2 and 1 5) . Probes for isolating DNA encoding a human σ 1 H subunit are preferably 14 or 1 6 contiguous nucleotides in length. In some instances, probes of 30 or 50 nucleotides are used and in other instances probes between 50 to 100 nucleotides are used. Eukaryotic cells containing heterologous DNA encoding one or more calcium channel subunits, particularly human calcium channel subunits, or containing RNA transcripts of DNA clones encoding one or more of the subunits are provided. A single σ 1 H subunit can form a channel. The requisite combination of subunits for formation of active channels in selected cells, however, can be determined empirically using the methods herein. For example, if a selected a subtype or variant does not form an active channel in a selected cell line, an additional subunit or subunits can be added until an active channel is formed. Other subunits can be added to assess the effects of such addition. In preferred embodiments, the cells contain DNA or RNA encoding an a subunit, preferably an σ 1 H subunit of an animal, preferably of a mammalian calcium channel. Embodiments in which the cells contain nucleic acid encoding an σ 1 H are of particular interest herein. In other embodiments, the cells contain DNA or RNA encoding additional heterologous subunits, including an a 2 δ. The cells may also include nucleic acid encoding a β subunit and/or a y subunit. In such embodiments, eukaryotic cells stably or transiently transfected with any combination of one, two, three or four of the subunit-encoding DNA clones, such as DNA encoding any of a a + β, a + β + a 2 , are provided . The eukaryotic cells provided herein contain heterologous nucleic acid that encodes an a subunit and optionally a heterologous a 2 - subunit and/or a β subunit and/or y subunit. In preferred embodiments, the cells express such heterologous calcium channel subunits and include one or more of the subunits in membrane-spanning heterologous calcium channels. In more preferred embodiments, the eukaryotic cells express functional, heterologous calcium channels that are capable of gating the passage of calcium channel-selective ions and/or binding compounds that, at physiological concentrations, modulate the activity of the heterologous calcium channel. In certain embodiments, the heterologous calcium channels include at least one heterologous calcium channel subunit. In most preferred embodiments, the calcium channels that are expressed on the surface of the eukaryotic cells are composed substantially or entirely of subunits encoded by the heterologous DNA or RNA. In preferred embodiments, the heterologous calcium channels of such cells are distinguishable from any endogenous calcium channels of the host cell. Such cells provide a means to obtain homogeneous populations of calcium channels. Typically, the cells contain the selected calcium channel as the only heterologous ion channel expressed by the cell. In certain embodiments the recombinant eukaryotic cells that contain the heterologous DNA encoding the calcium channel subunits are produced by transfection with DNA encoding one or more of the subunits or are injected with RNA transcripts of DNA encoding one or more of the calcium channel subunits. The DNA may be introduced as a linear DNA fragment or may be included in an expression vector for stable or transient expression of the subunit-encoding DNA. Vectors containing DNA encoding human calcium channel subunits are also provided. The eukaryotic cells that express heterologous calcium channels may be used in assays for calcium channel function or, in the case of cells transformed with fewer subunit-encoding nucleic acids than necessary to constitute a functional recombinant human calcium channel, such cells may be used to assess the effects of additional subunits on calcium channel activity. The additional subunits can be provided by subsequently transfecting such a cell with one or more DNA clones or RNA transcripts encoding human calcium channel subunits. The recombinant eukaryotic cells that express membrane spanning heterologous calcium channels may be used in methods for identifying compounds that modulate calcium channel activity. In particular, the cells are used in assays that identify agonists and antagonists of calcium channel activity in humans and/or assessing the contribution of the various calcium channel subunits to the transport and regulation of transport of calcium ions. Because the cells constitute homogeneous populations of calcium channels, they provide a means to identify agonists or antagonists of calcium channel activity that are specific for each such population. The cells provided herein may be used to assess T-type channel function and tissue distribution and to identify compounds that modulate the activity of T-type channels. Because T-type channels are operative in neurons in the thalamus, hypothalamus, and brain stem, and may be involved in autonomic nervous functions, in regulation of cardiovascular activities such as heart rate, arterial and venous smooth muscle innervation and tone, pulmonary rate and other fundamental processes, assays designed to assess such activities and assays the identify modulators of these activities provide a means to understand fundamental physiological processes and also a means to identify new drug candidates for an array of disorders. Assays that use the eukaryotic cells for identifying compounds that modulate calcium channel activity are also provided. In practicing these assays the eukaryotic cell that expresses a heterologous calcium channel, containing at least one subunit encoded by the DNA provided herein, is in a solution containing a test compound and a calcium channel selective ion, the cell membrane is depolarized, and current flowing into the cell is detected. If the test compound is one that modulates calcium channel activity, the current that is detected is different from that produced by depolarizing the same or a substantially identical cell in the presence of the same calcium channel-selective ion but in the absence of the compound. In preferred embodiments, prior to the depolarization step, the cell is maintained at a holding potential which substantially inactivates calcium channels which are endogenous to the cell. Also in preferred embodiments, the cells are mammalian cells, most preferably HEK cells, or amphibian oδcytes. Cells that express T-channels or LVA channels may be used in assays that screen for compounds that have activity as modulators, particularly antagonists, of the activity of these channels. Transcription based assays for identifying compounds that modulate the activity of calcium channels (see, U.S. Patent Nos. 5,436, 1 28 and 5,401 ,629), particularly calcium channels that contain an σ 1 H subunit are provided. These assays use cells that express calcium channels, particularly calcium channels containing an σ 1 H -subunit, and more preferably an σ 1 H -subunit encoded by heterologous DNA, and also contain nucleic acid encoding a reporter gene construct containing a reporter gene in operative linkage with one or more transcriptional control elements that is regulated by a calcium channel. The assays are effected by comparing the difference in the amount of transcription of a the reporter gene in the cells provided herein in the presence of the compound with the amount of transcription in the absence of the compound, or with the amount of transcription in the absence of the heterologous calcium channel, whereby compounds that modulate the activity of the heterologous calcium channel in the cell are identified. The reporter gene is any such gene known to those of skill in the art, including, but not limited to the gene encoding bacterial chloramphenicol acetyltransferase, the gene encoding firefly luciferase, the gene encoding bacterial luciferase, the gene encoding ?-galactosidase or the gene encoding alkaline phosphatase, and the transcriptional control element is any such element known to those of skill in the art, including, but not limited to serum responsive elements, cyclic adenosine monophosphate responsive elements, the c-fos gene promoter, the vasoactive intestinal peptide gene promoter, the somatostatin gene promoter, the proenkephalin promoter, the phosphoenolpyruvate carboxykinase gene promoter or the nerve growth factor-1 A gene promoter and elements responsive to intracellular calcium ion levels. Other assays in which receptor activity in response to test compounds is measured may also be practiced with the cells provided herein (see, e.g., U.S. Patent No. 5,670, 1 1 3) . Because T-type channels appear to be associated with a variety of key functions, cells that express T-channels and assays using such cells will be useful for identification of compounds for treatment of a variety of disorders, disease and conditions. Identified compounds will be candidates for use in the treatment of disorders and conditions associated with T-channel activity. Such activities include, but are not limited to, those involving role in muscle excitability, secretion and pacemaker activity, Ca 2 + dependent burst firing, neuronal oscillations, and potentiation of synaptic signals, for improving arterial compliance in systolic hypertension, or improving vascular tone, such as by decreasing vascular welling, in peripheral circulatory disease, and others. Other disorders include, but are not limited to hypertension, cardiovascular disorders, including but not limited to: myocardial infarct, cardiac arrhythmia, heart failure and angina pectoris; neurological disorders, such as schizophrenia, epilepsy and depression, peripheral muscle disorders, respiratory disorders and endocrine disorders. In particular, cells that express LVA channels, such as the σ 1H subunits, are useful for identifying compounds that are candidates for treatment of disorders associated with conduction tissues, such as atrial pacemaker cells, Purkinje fibers, and also coronary smooth muscles. Such disorders include, but are not limited to, compounds useful for treatment of cardiovascular, such as angina, vascular, such as hypertension, and urologic, hepatic, reproductive, adjunctive therapies for reestablishing normal heart rate and cardiac output following traumatic injury, heart attack and other cardiac injuries; treatments of myocardial infarct (Ml), post-MI and in an acute setting . Other compounds that interact with LVA, particularly T-type, calcium channels, may be effective for increasing cardiac contractile force, such as measured by left ventricular end diastolic pressure, and without changing blood pressure or heart rate. In an acute other compounds may be effective to decrease formation of scar tissue, such as that measured by collagen deposition or septal thickness, and without cardiodepressant effects. The assays may identify compounds useful in regulating vascular smooth muscle tone, either vasodilating or vasoconstricting in: (a) treatments for reestablishing blood pressure control, e.g., following traumatic injury, surgery or cardiopulmonary bypass, and in prophylactic treatments designed to minimize cardiovascular effects of anaesthetic drugs; (b) treatments for improving vascular reflexes and blood pressure control by the autonomic nervous system; for identifying compounds useful in treating urological disorders: (a) treating and restoring renal function following surgery, traumatic injury, uremia and adverse drug reactions; (b) treating bladder dysfunctions; and (c) uremic neuronal toxicity and hypotension in patients on hemodialysis; reproductive disorders, for identifying compounds useful in treating: (a) disorders of sexual function including impotence; (b) alcoholic impotence (under autonomic control that may be subject to T- channel controls); hepatic disorders for identifying compounds useful in treating and reducing neuronal toxicity and autonomic nervous system damage resulting from acute over-consumption of alcohol; neurologic disorders for identifying compounds useful in treating: (a) epilepsy and diencephalic epilepsy; (b) Parkinson's disease; (c) aberrant temperature control, such as, abnormalities of shivering and sweat gland secretion and peripheral vascular blood supply; (d) aberrant pituitary and hypothalamic functions including abnormal secretion of noradrenaline, dopamine and other hormones; for respiratory such as in treating abnormal respiration, e.g., post-surgical complications of anesthetics; and endocrine disorders, for identifying compounds useful in treating aberrant secretion of hormones including e.g., possible treatments for overproduction of insulin, thyroxin, adrenalin, and other hormonal imbalances. Purified human σ 1 H calcium channel subunits and purified human calcium channels containing such subunits are provided. The subunits and channels can be isolated from a eukaryotic cell transfected with nucleic acid that encodes the subunit. In another embodiment, immunoglobulins or antibodies obtained from the serum of an animal immunized with a substantially pure preparation of a human calcium channel, human calcium channel subunit or epitope-containing fragment of a human calcium subunit are provided. Monoclonal antibodies produced using a human calcium channel, human calcium channel subunit or epitope-containing fragment thereof as an immunogen are also provided . E. co/ fusion proteins including a fragment of a human calcium channel subunit may also be used as immunogen. Such fusion proteins may contain a bacterial protein or portion thereof, such as the E. coli TrpE protein, fused to a calcium channel subunit peptide. The immunoglobulins that are produced using the calcium channel subunits or purified calcium channels as immunogens have, among other properties, the ability to specifically and preferentially bind to and/or cause the immunoprecipitation of a human calcium channel or a subunit thereof which may be present in a biological sample or a solution derived from such a biological sample. Such antibodies may also be used to selectively isolate cells that express calcium channels that contain the subunit for which the antibodies are specific. Methods for modulating the activity of ion channels by contacting the calcium channels with an effective amount of the above-described antibodies are also provided. Thus, assays for identifying compounds that modulate the activity of LVA calcium channels, particularly T-type channels are provided as well as compounds identified by the methods. Also provided are methods for diagnosing LVA calcium channel- mediated, particularly T-type channel-mediated, disorders. Methods of diagnosis will involve detection of aberrant channel expression or function, such altered amino acid sequences, altered pharmacological profiles and altered electrophysiological profiles compared to normal or wild-type channels. Such methods typically can employ antibodies specific for the altered channel or nucleic acid probes to detect altered genes or transcripts. DESCRIPTION OF THE FIGURES FIGURE 1 shows the voltage-dependence of activation (m∞) and steady-state inactivation (h) of human 1 H calcium channels expressed transiently in HEK cells. Voltage-dependence of activation (m∞) was determined from tail current analysis. Tail currents were normalized with respect to the maximum peak tail current obtained at + 60 mV and were plotted (open symbols, mean ± SEM; n = 1 1 ) vs. test potential. Data were fitted by the sum of two Boltzman function m∞ = FA*[1 + exp (- (Vtest-V1 /2,A)/KA)] 1 + F B * [1 + exp(-(V test -V 1 /2 B )/k B )]-\ F A = 0.67, V 1 /2 A = - 21 .5mV, k A = 7.5, F B = 0.33, V 1/2 B = 25.5 mV, k B = 14.7. Steady-state inactivation (h ∞) was determined from a holding potential of -1 00 mV by a test pulse to -20 mV (p1 ), followed by a 20 second prepulse from -100 mV to -1 0 mV in 5 mV decrements (pHold) preceding a second test pulse to -20 mV (p2) . Normalized current amplitudes were plotted (closed symbols, mean ± SEM; n = 9) vs. holding potential. Data were fitted by a Boltzman function hoo = [1 + V 1/2 = -63.9 mV, k = 3. 9mV. FIGURE 2 shows the kinetics of activation (FIGURE 2A) and inactivation (FIGURE 2B) of human σ 1 H (α 1 H -ι) calcium channels; kinetics of activation and inactivation were determined from current traces by fitting an exponential function to rising (FIG. 2A) or declining (FIG. 2B) phase of the current (the voltage-dependence for activation and inactivation follows approximately an exponential function). FIGURE 3 schematically depicts features of the a H subunit and shows amino acid sequence alignment of human σ 1 H with σ 1 D and σ 1 E in each of the four pore regions; indicates residues involved in ion selectivity in each of the four pore regions; the unusually large loop in the LVA-associated σ 1 H subunits between transmembrane domains I and II. FIGURE 4A shows the tail currents elicited by repolarization to -90 mV following 10 ms step depolarizations between -80 and -10 mV. For tail current measurements the digitization/filter rates were 50/1 6 kHz. Tail current decay was fitted to a bi-exponential function of the form l = A 0 + A, exp(-t/r 1 ) + A 2 exp(-t/r 2 ) . The bi-exponential decay profile of the tail current was observed in every cell examined (n = 1 2) . FIGURES 4B and 4C show the voltage-dependence of the time constants τ and τ 2 for current deactivation (FIGURE 4B) and the current fractions A, and A 2 (FIGURE 4C) . DETAILED DESCRIPTION OF THE INVENTION Definitions: Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs. All patents and publications referred to herein are incorporated by reference herein. Reference to each of the calcium channel subunits includes the subunits that are specifically disclosed herein and human calcium channel subunits encoded by nucleic acid that can be isolated by using the nucleic acid disclosed as probes and screening an appropriate human cDNA or genomic library under at least low stringency, preferably high stringency. Such DNA also includes DNA that encodes proteins that have about 40% homology, typically at least about 90% sequence identity taking into account gaps) to any of the subunits proteins described herein or DNA or RNA that hybridizes under conditions of at least low stringency to the DNA provided herein and the protein encoded by such DNA exhibits additional identifying characteristics, such as function or molecular weight. In particular, reference to an σ 1 H subunit refers to subunits that can be isolated from nucleic acid libraries from any desired source using the nucleic acid disclosed herein as a probe. The encoded subunit is characterized by the presence of the notably long intracellular loop between transmembrane domains I and II, and/or properties ascribed to T- type or LVA type channels. It is understood that subunits that are encoded by transcripts that represent splice variants of the disclosed subunits or other such subunits may exhibit less than 40% overall homology to any single subunit, but will include regions of such homology to one or more such subunits. It is also understood that 40% homology refers to proteins that share approximately 40% of their amino acids in common or that share somewhat less, but include conservative amino acid substitutions, whereby the activity of the protein is not substantially altered. The subunits and DNA fragments encoding such subunits are provided herein or known to those of skill in the art (see, published International PCT application Nos. WO89/09834, WO93/04083, WO95/04822, U.S. Patent Nos. 5,792,846, 5,726,035, 5,407,820, 5,686,241 , 5,61 8,720, 5,710,250, 5,429,921 , 5,429,921 and 5,386,025) include any a a 2 , β or y subunits of a human calcium channel. Nucleic acid encoding LVA subunits, particularly 1 H subunits of human and other animal calcium channels, are provided herein. In particular, such DNA fragments include any isolated DNA fragment that (encodes a subunit of a human calcium channel, that (1 ) contains a sequence of nucleotides that encodes the subunit, and (2) is selected from among: (a) a sequence of nucleotides that encodes a human calcium σ 1 H channel subunit and includes a sequence of nucleotides set forth in any of the SEQ ID's herein (i.e. , SEQ ID Nos. 1 2, 1 5 and 1 6) that encodes such subunit; (b) a sequence of nucleotides that encodes the subunit and hybridizes under conditions of high stringency to DNA that is complementary to an mRNA transcript present in a human cell that encodes a LVA subunit, particularly an σ 1 H -subunit; (c) a sequence of nucleotides that encodes the subunit that includes a sequence of amino acids encoded by any of SEQ ID Nos. 1 2-1 6; and (d) a sequence of nucleotides that encodes a subunit that includes a sequence of amino acids encoded by a sequence of nucleotides that encodes such subunit and hybridizes under conditions of high stringency to DNA that is complementary to an mRNA transcript present in a human cell that encodes the subunit that includes a sequence of nucleotides set forth in any of SEQ ID Nos. 1 2-1 6. As used herein, the σ, subunit types, encoded by different genes, are designated as type σ 1A , α 1 B , σ 1c , σ 1 D , σ 1E and σ 1H . These types have also been referred to as VDCC IV for σ 1 B , VDCC II for σ 1 c and VDCC III for σ 1 D . Subunit subtypes, which are splice variants, are referred to, for example as a H , σ 1 H . 2 , σ 1 B .., , σ 1 B 2 , a C etc. Thus, as used herein, nucleic acid (DNA or RNA) encoding the σ 1 subunit refers to nucleic acid that hybridizes to the DNA provided herein under conditions of at least low stringency, typically high stringency, or encodes a subunit that has at least about 40% homology to protein encoded by DNA disclosed herein that encodes the specified σ, subunit of a human calcium channel. In the case of LVA channels, nucleic acid that encodes a subunit that hybridizes under at least low stringency, preferably high stringency, to nucleic acid that encodes an σ 1 H subunit, and that encodes a subunit having the requisite LVA properties in assays for such activity, as those described herein. Splice variants will have varying percentages of overall homology (or identity), but will be derived from the same gene and will include regions of 1 00% identity. In particular, a splice variant of any of the σ, subunits (or any of the subunits particularly disclosed herein) will contain regions (at least one exon) of divergence and one or more regions (at least one exon, typically more than about 1 6 nucleotides, and generally substantially more) that have 100% homology with one or more of the σ, subunit subtypes provided herein, and will also contain a region that has substantially less homology, since it is derived from a different exon. It is well within the skill of those in this art to identify exons and splice variants. Thus, for example, an σ 1 H subunit will be readily identifiable, because it will share at least about 40% protein homology with one of the σ 1H subunits disclosed herein, and will include at least one region (one exon) that is 100% homologous. It will also have activity, as discussed below, that indicates that it is an LVA a subunit. It is noted herein, that identity and homology refer to the percentage of amino acids when proteins are compared or nucleotides when nucleic acids are compared that are shared. Numerous computer programs for determining identity are available. In all instances, intended gap penalties and other parameters are the defaults set by the manufacturer. Although not really needed when there is a high (90% or greater) degree of identity between sequences such programs include, but are not limited to commercially available sequence alignment programs, such as the DNAStar "MegAlign" program (Madison, Wl) and the University of Wisconsin Genetics Computer Group (UWG) "Gap" program (Madison Wl), to determine a percentage of sequence identity (see, also, von Heijne, entitled "Sequence Analysis in Molecular Biology: Treasure Trove of Trivial Pursuit" Academic Press ( 1 987) Appendix 2 (citing to UWG and DNAStar among seven commercially available software programs)) . An a subunit may be identified by its ability to form a calcium channel. Typically, a subunits have molecular masses greater than at least about 1 20 kD. Also, hydropathy plots of deduced a subunit amino acid sequences indicate that the a subunits contain four internal repeats, each containing six transmembrane domains. An σ 1 H -subunit is identified by its pore-forming ability and also the low-voltage activation of the resulting channel. The activity of a calcium channel may be assessed in vitro by methods known to those of skill in the art, including the electrophysiological and other methods described herein. Typically, σ, subunits include regions with which one or more modulators of calcium channel activity, such as a 1 ,4-DHP or α/-CgTx, interact directly or indirectly. Types of σ, subunits may be distinguished by any method known to those of skill in the art, including on the basis of binding specificity. For example, it has been found herein that σ 1 B subunits participate in the formation of channels that have previously been referred to as N-type channels, σ 1 D subunits participate in the formation of channels that had previously been referred to as L-type channels, σ 1 A subunits appear to participate in the formation of channels that exhibit characteristics typical of channels that had previously been designated P- type channels, and σ 1 H subunits appear to participate in channels that exhibit activities associated with T-type channels. Thus, for example, the activity of channels that contain the σ 1 B subunit are insensitive to 1 ,4- DHPs; whereas the activity of channels that contain the σ 1 D subunit are modulated or altered by a 1 ,4-DHP. It is presently preferable to refer to calcium channels based on pharmacological characteristics and current kinetics and to avoid historical designations. Types and subtypes of a subunits may be characterized on the basis of the effects of such modula- tors on the subunit or a channel containing the subunit as well as differences in currents and current kinetics produced by calcium channels containing the subunit. The σ 1 H subunits may be further identified by the presence the notably long intracellular loop regions, such as between transmembrane domains I and II (e.g ., nt 1 506 to nt 2627 of SEQ ID No. 1 2), and also the loop in domain I. In particular, nucleic acid that encodes an σ 1 H subunit as used herein, will hybridize under conditions of high stringency to the nucleic acid disclosed herein as SEQ ID Nos. 1 2, 1 5 and 1 6, and will form a channel in a mammalian cell, such as an HEK cell, that exhibits electrophysiological and/or pharmacological properties of a LVA or T- channel. The electrophysiological properties include one or more of the following electrophysiological properties a relative conductance of Ba 2 + of about 5 pS (picoseconds) to about 9 pS, an activation time of about 2 to about 8 milliseconds, a kinetics of activation V 1 /2 value of about -60 millivolts to about 26 millivolts, an inactivation time of about 1 0 to about 30 milliseconds, a kinetics of inactivation V 1 /2 value of about -100 millivolts to about -500 millivolts, and a tail deactivation time of about 2 to about 1 2 milliseconds. In addition, the resulting channel may have pharmacological properties, such as a relatively high degree of sensitivity to mibefradil, (IS,2S)-2-[2-[[3-( 1 H-benzimidazol-2-yl)propyl]methyl-amino]ethyl]-6-fluoro- 1 -isopropyl-1 ,2,3,4-tetrahydronaphthalen-2-yl methoxyacetate (Hoffman- LaRoche, Inc.) and/or a relatively high degree of resistance to the Conus snail toxins GVIA and MVIIC as well as the arachnid toxins AgalllA and AgalVA compared to HVA calcium channels. As used herein, an a 2 subunit is encoded by nucleic acid (DNA or RNA) disclosed, for example, in U.S. Patent No. 5,407,820, U.S. Patent No. 5,792,846 and International PCT application No. WO95/04822 that encodes an a 2 subunit of a mammalian calcium channel or that hybridizes to DNA under conditions of low stringency, preferably high stringency, or encodes a protein that has at least about 40% homology, typically at least about 90% identity, taking into account gaps, with that disclosed therein. Such DNA encodes a protein that typically has a molecular mass greater than about 1 20 kD, but does not form a calcium channel in the absence of an a subunit, and may alter the activity of a calcium channel that contains an a subunit. Subtypes of the a 2 subunit that arise as splice variants are designated by lower case letter, such as σ 2a , . . . σ 2e . In addition, the a 2 subunit and the large fragment produced when the protein is subjected to reducing conditions appear to be glycosylated with at least N-linked sugars and do not specifically bind to the 1 ,4-DHPs and phenylalkylamines that specifically bind to the a Λ subunit. The smaller fragment, the C-terminal fragment, is referred to as the δ subunit and includes amino acids from about 946 (as numbered in International PCT application No. WO95/04822, e.g. , SEQ ID No. 1 1 therein) through about the C-terminus. This fragment may dissociate from the remaining portion of a 2 when the a 2 subunit is exposed to reducing conditions. For purposes herein a 2 is also referred to as a 2 δ. Thus, reference to a 2 δ means the a 2 subunit, including the C-terminal δ portion. As used herein, a β subunit is encoded by DNA disclosed, for example, in U.S. Patent No. 5,407,820, U.S. Patent No. 5,792,846 and International PCT application No. WO95/04822 or that hybridizes to the DNA provided therein under conditions of low stringency, preferably high stringency, or encodes a protein that has at least about 40% homology, typically about at least about 90% homology) with that disclosed therein and is a protein that typically has a molecular mass lower than the a sub- units and on the order of about 50-80 kD, does not form a detectable calcium channel in the absence of an σ, subunit, but may alter the activity of a calcium channel that contains an a subunit or that contains an a and a 2 subunit. Types of the β subunit that are encoded by different genes are designated with subscripts, such as β β 2 , β 3 and ? 4 . Subtypes of β subunits that arise as splice variants of a particular type are designated with a numerical subscript referring to the type and to the variant. Such subtypes include, but are not limited to the ?, splice variants, including ?,_ -? 1 5 and β 2 variants, including /? 2C - ? 2E - As used herein, a y subunit is a subunit of calcium channel encoded by DNA disclosed for example in U.S. Patent Nos. 5,726,035 and 5,386,025; see, also Jay et al. ( 1 990) Science 248:490-492 and Lett et al. ( * 1 998) Nature Genetics 1 9:340-347) and may be isolated and identified using the nucleic disclosed therein as a probe by hybridization or other such method known to those of skill in the art, whereby full- length clones encoding a y subunit may be isolated or constructed. A y subunit will be encoded by nucleic acid that hybridizes to the DNA provided therein under conditions of low stringency, preferably high stringency, exhibits sufficient sequence homology to encode a protein that has at least about 40% homology with the y subunit described herein. Thus, one of skill in the art, in light of the disclosure herein, can identify DNA encoding a , a 2 , β, δ and y calcium channel subunits, including types encoded by different genes and subtypes that represent splice variants. For example, DNA or RNA probes based on the DNA disclosed herein may be used to screen an appropriate library, including a genomic or cDNA library, for hybridization to the probe and obtain DNA in one or more clones that includes an open reading fragment that encodes an entire protein. Subsequent to screening an appropriate library with the DNA disclosed herein, the isolated DNA can be examined for the presence of an open reading frame from which the sequence of the encoded protein may be deduced. Determination of the molecular weight and comparison with the sequences herein should reveal the identity of the subunit as an a a 2 etc. subunit. Functional assays may, if necessary, be used to determine whether the subunit is an a a 2 subunit or β subunit. For example, DNA encoding an α 1 A subunit may be isolated by screening an appropriate library with DNA, encoding all or a portion of the human σ 1A subunit. Such DNA includes the DNA in the phage deposited under ATCC Accession No. 75293 that encodes a portion of an a subunit. DNA encoding an σ 1 A subunit may be obtained from an appropriate library by screening with an oligonucleotide having all or a portion of the sequence of an σ 1A subunit (see, e.g. , published International PCT application No. WO95/04822, particularly SEQ ID Nos. 21 , 22 and/or 23 or with the DNA in the deposited phage therein) . Alternatively, such DNA may have the coding sequence that encodes an αr 1 A subunit. Any method known to those of skill in the art for isolation and identification of DNA and preparation of full-length genomic or cDNA clones, including methods exemplified herein, may be used. DNA encoding σ 1 H can be isolated by screening a human medullary thyroid carcinoma cell line (TT cells) or other suitable library human cDNA library with DNA probes prepared from nucleic acid provided herein. Full- length clones are constructed and expressed as described and exemplified herein and the resulting channels tested to verify that the encoding nucleic acid encodes a LVA channel. The subunit encoded by isolated DNA may be identified by comparison with the DNA and amino acid sequences of the subunits provided herein. Splice variants share extensive regions of homology, but include non-homologous regions, subunits encoded by different genes share a uniform distribution of non-homologous sequences. As used herein, a splice variant refers to a variant produced by differential processing of a primary transcript of genomic DNA that results in more than one type of mRNA. Splice variants may occur within a single tissue type or among tissues (tissue-specific variants). Thus, cDNA clones that encode calcium channel subunit subtypes that have regions of identical amino acids and regions of different amino acid sequences are referred to herein as "splice variants" . As used herein, a "calcium channel-selective ion" is an ion that is capable of flowing through, or being blocked from flowing through, a calcium channel which spans a cellular membrane under conditions which would substantially similarly permit or block the flow of Ca 2 + . Ba 2 + is an example of an ion which is a calcium channel-selective ion. As used herein, a compound that modulates calcium channel activity is one that affects the ability of the calcium channel to pass calcium channel-selective ions or affects other detectable calcium channel features, such as current kinetics. Such compounds include calcium channel antagonists and agonists and compounds that exert their effect on the activity of the calcium channel directly or indirectly. As used herein, a "substantially pure" subunit or protein is a subunit or protein that is sufficiently free of other polypeptide contaminants to appear homogeneous by SDS-PAGE or to be unambiguously sequenced. As used herein, selectively hybridize means that a DNA fragment hybridizes to a second fragment with sufficient specificity to permit the second fragment to be identified or isolated from among a plurality of fragments. In general, selective hybridization occurs at conditions of high stringency. As used herein, heterologous or foreign DNA and RNA are used interchangeably and refer to DNA or RNA that does not occur naturally as part of the genome in which it is present or which is found in a location or locations in the genome that differ from that in which it occurs in nature. It is DNA or RNA that is not endogenous to the cell and has been artificially introduced into the cell. Examples of heterologous DNA include, but are not limited to, DNA that encodes a calcium channel subunit and DNA that encodes RNA or proteins that mediate or alter expression of endogenous DNA by affecting transcription, translation, or other regulatable biochemical processes. The cell that expresses the heterologous DNA, such as DNA encoding a calcium channel subunit, may contain DNA encoding the same or different calcium channel subunits. The heterologous DNA need not be expressed and may be introduced in a manner such that it is integrated into the host cell genome or is maintained episomally. As used herein, operative linkage of heterologous DNA to regulatory and effector sequences of nucleotides, such as promoters, enhancers, transcriptional and translational stop sites, and other signal sequences, refers to the functional relationship between such DNA and such sequences of nucleotides. For example, operative linkage of heterologous DNA to a promoter refers to the physical and functional relationship between the DNA and the promoter such that the transcription of such DNA is initiated from the promoter by an RNA polymerase that specifically recognizes, binds to and transcribes the DNA in reading frame. As used herein, isolated, substantially pure DNA refers to DNA fragments purified according to standard techniques employed by those skilled in the art (see, e.g. , Maniatis et al. ( 1 982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY) . As used herein, expression refers to the process by which nucleic acid is transcribed into mRNA and translated into peptides, polypeptides, or proteins. If the nucleic acid is derived from genomic DNA, expression may, if an appropriate eukaryotic host cell or organism is selected, include splicing of the mRNA. As used herein, vector or plasmid refers to discrete elements that are used to introduce heterologous DNA into cells for either expression of the heterologous DNA or for replication of the cloned heterologous DNA. Selection and use of such vectors and plasmids are well within the level of skill of the art. As used herein, expression vector includes vectors capable of expressing DNA fragments that are in operative linkage with regulatory sequences, such as promoter regions, that are capable of effecting expression of such DNA fragments. Thus, an expression vector refers to a recombinant DNA or RNA construct, such as a plasmid, a phage, recombinant virus or other vector that, upon introduction into an appropriate host cell, results in expression of the cloned DNA. Appropriate expression vectors are well known to those of skill in the art and include those that are replicable in eukaryotic cells and/or prokaryotic cells and those that remain episomal or may integrate into the host cell genome. As used herein, a promoter region refers to the portion of DNA of a gene that controls transcription of the DNA to which it is operatively linked. The promoter region includes specific sequences of DNA that are sufficient for RNA polymerase recognition, binding and transcription initiation. This portion of the promoter region is referred to as the promoter. In addition, the promoter region includes sequences that modulate this recognition, binding and transcription initiation activity of the RNA polymerase. These sequences may be cis acting or may be responsive to trans acting factors. Promoters, depending upon the nature of the regulation, may be constitutive or regulated. As used herein, a recombinant eukaryotic cell is a eukaryotic cell that contains heterologous DNA or RNA. As used herein, a recombinant or heterologous calcium channel refers to a calcium channel that contains one or more subunits that are encoded by heterologous DNA that has been introduced into and expressed in a eukaryotic cell that expresses the recombinant calcium channel. A recombinant calcium channel may also include subunits that are produced by DNA endogenous to the cell. In certain embodiments, the recombinant or heterologous calcium channel may contain only subunits that are encoded by heterologous DNA. As used herein, "functional" with respect to a recombinant or heterologous calcium channel means that the channel is able to provide for and regulate entry of calcium channel-selective ions, including, but not limited to, Ca 2 + or Ba 2 + , in response to a stimulus and/or bind ligands with affinity for the channel. Preferably such calcium channel activity is distinguishable, such as by electrophysiological, pharmacological and other means known to those of skill in the art, from any endogenous calcium channel activity that is in the host cell. As used herein, a T-type channel or LVA type channel typically refers to a calcium channel that exhibits a low-threshold calcium current that is activated and inactivated at low voltages compared to calcium channels (such as those that include an σ 1 D subunit) referred to as high voltage activated (HVA) channels. In addition or alternatively, a T-type channel may be characterized by distinct biophysical features, such as slow deactivation rates, very low conductances (5-9 pS) and voltage- dependent inactivation. T channels may exhibit a relatively high degree of sensitivity to mibefradil (Hoffman-LaRoche, Inc.) and/or a relatively high degree of resistance to the Conus snail toxins GVIA and MVIIC as well as the arachnid toxins AgalllA and AgalVA compared to HVA calcium channels. These channels also typically exhibit reduced affinity for cadmium. T-type channels or LVA type channels may also be characterized at the nucleic acid level by the presence of one or more extended intracellular loops (see, e.g ., SEQ ID NO. 1 2, 1 5 and 1 6 ) between transmembrane domains, such as between transmembrane domains I and II. As used herein, a polypeptide having an amino acid sequence substantially as set forth in a particular SEQ ID No. includes protein that may have the same function but may include minor variations in sequence, such as conservative amino acid changes or minor deletions or insertions that do not alter the activity of the protein. The activity of a calcium channel receptor subunit protein, particularly a LVA or T-type channel, refers to its ability to form a functional calcium channel alone or with other subunits. A T-type channel will have the distinguishing properties defined herein. As used herein, a physiological concentration of a compound is that which is necessary and sufficient for a biological process to occur. For example, a physiological concentration of a calcium channel-selective ion is a concentration of the calcium channel-selective ion necessary and sufficient to provide an inward current when the channels open. As used herein, activity of a calcium channel refers to the movement of a calcium channel-selective ion through a calcium channel. Such activity may be measured by any method known to those of skill in the art, including, but not limited to, measurement of the amount of current which flows through the recombinant channel in response to a stimulus. As used herein, a "functional assay" refers to an assay that identifies functional calcium channels. A functional assay, thus, is an assay to assess function. As understood by those skilled in the art, assay methods for identifying compounds, such as antagonists and agonists, that modulate calcium channel activity, generally require comparison to a control. One type of a "control" cell or "control" culture is a cell or culture that is treated substantially the same as the cell or culture exposed to the test compound except that the control culture is not exposed to the test compound . Another type of a "control" cell or "control" culture may be a cell or a culture of cells which are identical to the transfected cells except the cells employed for the control culture do not express functional calcium channels. In this situation, the response of test cell to the test compound is compared to the response (or lack of response) of the calcium channel-negative cell to the test compound, when cells or cultures of each type of cell are exposed to substantially the same reaction conditions in the presence of the compound being assayed . For example, in methods that use patch clamp electrophysiological procedures, the same cell can be tested in the presence and absence of the test compound, by changing the external solution bathing the cell as known in the art. It is also understood that each of the subunits disclosed herein may be modified by making conservative amino acid substitutions and the resulting modified subunits are contemplated herein. Suitable conservative substitutions of amino acids are known to those of skill in this art and may be made generally without altering the biological activity of the resulting molecule. Those of skill in this art recognize that, in general, single amino acid substitutions in non-essential regions of a polypeptide do not substantially alter biological activity (see, e.g., Watson et a _ Molecular Biology of the Gene, 4th Edition, 1 987, The Benjamin/Cummings Pub. Co., p.224) . Such substitutions are preferably, although not exclusively, made in accordance with those set forth in TABLE 1 as follows: TABLE 1 Original residue Conservative substitution Ala (A) Gly; Ser Arg (R) Lys Asn (N) Gin; His Cys (C) Ser Gin (Q) Asn Glu (E) Asp Gly (G) Ala; Pro His (H) Asn; Gin He (I) Leu; Val Leu (L) lie; Val Lys (K) Arg; Gin; Glu Met (M) Leu; Tyr; lie Phe (F) Met; Leu; Tyr Ser (S) Thr Thr (T) Ser Trp (W) Tyr Tyr (Y) Trp; Phe Val (V) lie; Leu Other substitutions are also permissible and may be determined empirically or in accord with known conservative substitutions. Any such modification of the polypeptide may be effected by any means known to those of skill in this art. Mutation may be effected by any method known to those of skill in the art, including site-specific or site- directed mutagenesis of DNA encoding the protein and the use of DNA amplification methods using primers to introduce and amplify alterations in the DNA template. As used herein, treatment means any manner in which the symptoms of a conditions, disorder or disease are ameliorated or otherwise beneficially altered. Treatment also encompasses any pharmaceutical use of the compositions herein, such as use as contraceptive agents. As used herein, a LVA-activated calcium channel-mediated disorder refers to disorders that are associated with LVA channel activities. A T- type calcium channel-mediated disorders LVA-activated channel-mediated disorders that are associated with T-type channels. Such disorders include, but are not limited to: cardiovascular, hepatic, endocrine, urologic, reproductive, muscular, neurological and other disorders in which LVA channels, particular T-type channels, play a role either in mediating the disorder in some manner contributing to it. As used herein, amelioration of the symptoms of a particular disorder by administration of a particular pharmaceutical composition refers to any lessening, whether permanent or temporary, lasting or transient that can be attributed to or associated with administration of the composition. As used herein, substantially pure means sufficiently homogeneous to appear free of readily detectable impurities as determined by standard methods of analysis, such as thin layer chromatography (TLC), gel electrophoresis and high performance liquid chromatography (HPLC), used by those of skill in the art to assess such purity, or sufficiently pure such that further purification would not detectably alter the physical and chemical properties, such as enzymatic and biological activities, of the substance. Methods for purification of the compounds to produce substantially chemically pure compounds are known to those of skill in the art. A substantially chemically pure compound may, however, be a mixture of stereoisomers. In such instances, further purification might increase the specific activity of the compound . As used herein, biological activity refers to the m . vivo activities of a compound or physiological responses that result upon in vivo administration of a compound, composition or other mixture. Biological activity, thus, encompasses therapeutic effects and pharmaceutical activity of such compounds, compositions and mixtures. Identification and isolation of DNA encoding human calcium channel subunits Methods for identifying and isolating nucleic acid (DNA and RNA) encoding a , a 2 , β and y, particularly nucleic acid encoding LVA a subunits of human calcium channels are provided. Identification and isolation of such nucleic acid may be accomplished by hybridizing, under appropriate conditions, at least low stringency, preferably high stringency, to restriction enzyme-digested human DNA with a labeled probe having at least 14, preferably 1 6 or more nucleotides (25, 30 or longer) and derived from any contiguous portion of DNA having a sequence of nucleotides set forth herein by sequence identification number. Once a hybridizing fragment is identified in the hybridization reaction, it can be cloned employing standard cloning techniques known to those of skill in the art. Full-length clones may be identified by the presence of a complete open reading frame and the identity of the encoded protein verified by sequence comparison with the subunits provided herein and by functional assays to assess calcium channel- forming ability or other function. This method can be used to identify genomic DNA encoding the subunit or cDNA encoding splice variants of human calcium channel subunits generated by alternative splicing of the primary transcript of genomic subunit DNA. For instance, DNA, cDNA or genomic DNA, encoding a calcium channel subunit may be identified by hybridization to a DNA probe and characterized by methods known to those of skill in the art, such as restriction mapping and DNA sequencing, and compared to the DNA provided herein in order to identify heterogeneity or divergence in the sequences of the DNA. Such sequence differences may indicate that the transcripts from which the cDNA was produced result from alternative splicing of a primary transcript, if the non-homologous and homologous regions are clustered, or from a different gene if the non-homologous regions are distributed throughout the cloned DNA. Splice variants share regions of 100% homology. As noted herein, the resulting nucleic acid may be expressed in cells and the resulting cells tested to verify or ascertain that expressed calcium channels exhibit pharmacological and/or electrophysiological properties of LVA or T-channels. Any suitable method for isolating genes using the DNA provided herein may be used. For example, oligonucleotides corresponding to regions of sequence differences have been used to isolate, by hybridization, DNA encoding the full-length splice variant and can be used to isolate genomic clones. A probe, based on a nucleotide sequence disclosed herein, which encodes at least a portion of a subunit of a human calcium channel, such as a tissue-specific exon, may be used as a probe to clone related DNA, to clone a full-length cDNA clone or genomic clone encoding the human calcium channel subunit. Labeled, including, but not limited to, radioactively or enzymatically labeled, RNA or single-stranded DNA of at least 1 4 substantially contiguous bases, preferably 1 6 or more, generally at least 30 contiguous bases of a nucleic acid which encodes at least a portion of a human calcium channel subunit, the sequence of which nucleic acid corresponds to a segment of a nucleic acid sequence disclosed herein by reference to a SEQ ID No. are provided. Such nucleic acid segments may be used as probes in the methods provided herein for cloning DNA encoding calcium channel subunits. See, generally, Sambrook et al. ( 1 989) Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press. In addition, nucleic acid amplification techniques, which are well known in the art, can be used to locate splice variants of calcium channel subunits by employing oligonucleotides based on DNA sequences surrounding the divergent sequence primers for amplifying human RNA or genomic DNA. Size and sequence determinations of the amplification products can reveal splice variants. Furthermore, isolation of human genomic DNA sequences by hybridization can yield DNA containing multiple exons, separated by introns, that correspond to different splice variants of transcripts encoding human calcium channel subunits. DNA encoding types and subtypes of each of the α α 2 , β and y subunits of voltage-dependent human calcium channels has been cloned by nucleic acid amplification of cDNA from selected tissues or by screening human cDNA libraries prepared from isolated poly A + mRNA from cell lines or tissue of human origin having such calcium channels. Among the sources of such cells or tissue for obtaining mRNA are human brain tissue or a human cell line of neural origin, such as a neuroblastoma cell line, human skeletal muscle or smooth muscle cells, and the like. Methods of preparing cDNA libraries are well known in the art (see generally Ausubel et al. (1987) Current Protocols in Molecular Biology, Wiley-lnterscience, New York; and Davis et al. ( 1 986) Basic Methods in Molecular Biology, Elsevier Science Publishing Co., New York) . Preferred regions from which to construct probes include 5' and/or 3' coding sequences, sequences predicted to encode transmembrane domains, sequences predicted to encode cytoplasmic loops, signal sequences, ligand-binding sites, and other functionally significant sequences (see Table, below) . Either the full-length subunit-encoding DNA or fragments thereof can be used as probes, preferably labeled with suitable label means for ready detection. When fragments are used as probes, preferably the DNA sequences will be typically from the carboxyl- end-encoding portion of the DNA, and most preferably will include predicted transmembrane domain-encoding portions based on hydropathy analysis of the deduced amino acid sequence (see, e.g. , Kyte and Doolittle (( 1 982) J. Mol. Biol. 1 67: 105) . Riboprobes that are specific for human calcium channel subunit types or subtypes have been prepared. These probes are useful for identifying expression of particular subunits in selected tissues and cells. The regions from which the probes were prepared were identified by comparing the DNA and amino acid sequences of all known a or β subunit subtypes. Regions of least homology, preferably human-derived sequences, and generally about 250 to about 600 nucleotides were selected. Numerous riboprobes for a and β subunits have been prepared (see, e.g. , Table 2 in International PCT application No. WO95/04822), which is repeated in part in the following Table. TABLE 2 SUMMARY OF RNA PROBES The pGEM ser es are ava lab e from Promega, Mad son Wl; see also, U.S. Patent No. 4,766,072. For the σ 1 H -specific probes (and also antibodies), regions unique to the σ 1 H subunits, such as the extended intracellular loops present in these channels may be used . For σ 1 H .-, specific antibodies the region present in σ 1 H .ι and absent from σ 1H . 2 may be useful for preparation of subunit- specific probes, purpose. The DNA clones and fragments thereof provided herein thus can be used to isolate genomic clones encoding each subunit and to isolate any splice variants by hybridization screening of libraries prepared from different human tissues. Nucleic acid amplification techniques, which are well known in the art, can also be used to locate DNA encoding splice variants of human calcium channel subunits. This is accomplished by employing oligonucleotides based on DNA sequences surrounding divergent sequence(s) as primers for amplifying human RNA or genomic DNA. Size and sequence determinations of the amplification products can reveal the existence of splice variants. Furthermore, isolation of human genomic DNA sequences by hybridization can yield DNA containing multiple exons, separated by introns, that correspond to different splice variants of transcripts encoding human calcium channel subunits. Once DNA encoding a calcium channel subunit is isolated, ribonuclease (RNase) protection assays can be employed to determine which tissues express mRNA encoding a particular calcium channel subunit or variant. These assays provide a sensitive means for detecting and quantitating an RNA species in a complex mixture of total cellular RNA. The subunit DNA is labeled and hybridized with cellular RNA. If complementary mRNA is present in the cellular RNA, a DNA-RNA hybrid results. The RNA sample is then treated with RNase, which degrades single-stranded RNA. Any RNA-DNA hybrids are protected from RNase degradation and can be visualized by gel electrophoresis and autoradiography. In situ hybridization techniques can also be used to determine which tissues express mRNA encoding a particular calcium channel subunit. The labeled subunit-encoding DNA clones are hybridized to different tissue slices to visualize subunit mRNA expression. With respect to each of the respective subunits (a a 2 , β or y) of human calcium channels, once the DNA encoding the channel subunit was identified by a nucleic acid screening method, the isolated clone was used for further screening to identify overlapping clones. Some of the cloned DNA fragments can and have been subcloned into an appropriate vector such as plBI24/25 (IBI, New Haven, CT), M 1 3mp 1 8/1 9, pGEM4, pGEM3, pGEM7Z, pSP72 and other such vectors known to those of skill in this art, and characterized by DNA sequencing and restriction enzyme mapping. A sequential series of overlapping clones may thus be generated for each of the subunits until a full-length clone can be prepared by methods, known to those of skill in the art, that include identification of translation initiation (start) and translation termination (stop) codons. For expression of the cloned DNA, the 5' noncoding region and other transcriptional and translational control regions of such a clone may be replaced with an efficient ribosome binding site and other regulatory regions as known in the art. Other modifications of the 5' end, known to those of skill in the art, that may be required to optimize translation and/or transcription efficiency may also be effected, if deemed necessary. Examples 1 -3 below, describe in detail the cloning DNA encoding σ 1 H splice variants and electrophyiological and pharmacological properties thereof. Except where noted, the methods of expression and other data is described with reference to the a H .^ encoding nucleic acid. It is understood that the exemplified methods may be used to isolate additional splice variants and related subunits from humans and other mammals and animals and may also be used to express such nucleic acid to produce cells for use in screening assays to identify compounds that modulate the activity of LVA activated channels, particularly T-type channels. The nucleic acid may also be used in diagnostic assays to identify mutations and to produce proteins and then antibodies for use as reagents in diagnostic assays for disorders associated with T-type calcium channel activities. σ 1 subunits of LVA channels Nucleic acid encoding σ, subunits that form LVA channels is provided herein. The nucleic acid provided herein may also be used to isolate related channels from other tissues, and other mammals and animals. Identification and isolation of DNA encoding the σ 1H human calcium channel subunits Calcium channels that contain σ 1 H should exhibit properties that differ from known HVA channels, formed from the σ 1 A - σ 1 E calcium channel subunits. Such differences may include low voltage activation, voltage-dependent inactivation, relatively high sensitivity to mibefradil and relatively high resistance to snail and arachnid toxins that inhibit most HVA channels (e.g., spider venom toxins ω-AgalllA and ω-AgalVA and the Conus snail toxin GVIA) . In addition σ 1 H -subunits may be identified by homology with other α^subunits and additionally by presence of an extended intracellular loop in the encoded subunit (see, e.g., SEQ No. 49, nucleotides 1 506-2627) located between transmembrane domains I and II . This region in σ 1 H is extended compared to other calcium channel σ, subunits, such as σ 1 A -σ 1 E . DNA encoding an α 1 H -subunit may be isolated using the DNA provided herein. In particular, probes of at least about 1 6 nucleotides or 30 nucleotides or other suitable length, such 14, 30, 1 00 etc. bases, may be used to screen selected libraries, including mammalian DNA libraries. The selected libraries are preferably prepared from mammalian tissue or cell sources known to express T-type channels. The sequence of the probe is preferably based on the sequence of the intracellular loop located between transmembrane domains I and II (see, e.g. , SEQ ID Nos. 1 2 and 1 5) . DNA encoding the σ 1 H subunit was isolated by amplifying a region of genes encoding an σ, subunit expressed in a human thyroid carcinoma cell line (TT cells) using degenerate oligonucleotide primers. The TT cell line is derived from a human medullary thyroid carcinoma and has been used to study calcitonin secretion and gene expression (deBustros et aL ( 1 986) J. Biol. Chem. 261 :8036-8041 ; deBustros et aL 1 990 Mol. Cell. Biol. 10: 1 773-1 778) . Whole-cell recordings from these cells reveal that the only voltage gated calcium channels expressed by these cells are low-voltage activated, rapidly inactivating and slowly deactivating, which are biophysical properties consistent with a T-type channel. A portion of one of the positive clones was used to further screen a human thyroid carcinoma cDNA library to identify overlapping clones that span the entire length of the nucleotide sequence encoding the human σ 1 H subunit. A full-length σ 1 H DNA clone can be constructed by ligating portions of the partial cDNA clones as described in Example 1 . SEQ ID No. 1 5 sets forth the nucleotide sequence of a clone encoding an σ 1 H ., subunit as well as the deduced amino acid sequence. Two splice variants, σ 1 H .., and σ 1 H . 2 , were detected by RT-PCR (reverse transcriptase-amplification) using RNA from multiple tissues. The σ 1 H 2 isoform (SEQ ID No. 1 6) contains a 957 nucleotide deletion, relative to σ 1 H .., (SEQ ID Nos. 1 2 and 1 5) in the l-ll intracellular loop, i.e, . (e.g ., nt 1 506 to nt 2627 of SEQ ID No. 1 2) . The σ 1 H .., subunit exhibits marked sequence differences, as well as certain structural similarities to previously cloned a subunits. Notably, the deduced amino acid sequence of a H shares less than 30% overall sequence identity with human σ 1 A -σ 1 E -encoding nucleic acids, which encode high-voltage activated calcium channels. Northern blot analysis indicates that mRNA transcripts for σ 1 H are expressed in the brain, primarily in the amygdala, caudate nucleus and putamen, and in peripheral tissues, primarily in the liver, kidney and heart. Specifically, a comparison of the nucleic acid and deduced amino acid sequences of this σ 1 H calcium channel subunit with other human a subunits reveals several distinct features. There are notable differences between σ 1 H and the HVA a Λ sequences. First, the intracellular loop between transmembrane Domains I and II is notably long . As exemplified in SEQ ID No. 49, the intracellular loop of human σ 1 H subunit is 1 , 1 22 nt in length whereas the corresponding intracellular loops in the other human a subunits described herein range from 351 to 381 nt in length. Thus, the intracellular loop of human σ 1 H is nearly 250 amino acids longer than human σ, subunits found in HVA calcium channels. The deduced amino acid sequence of this region (aa 420 to aa 794 of SEQ ID No. 1 2) contains a large number of proline residues and includes a poly-HIS region of 9 contiguous histidine residues (aa 52 to aa 528 of SEQ ID No. 1 2) and a region where 8 of 10 residues are alanine. The large intracellular loop located between transmembrane Domains I and II resembles the large intracellular loops found in a corresponding location in sodium channel a subunits some of which may function as homomers. It has been proposed that T-type channels have an activity that is a hybrid between HVA calcium channels and sodium channel. The σ 1 H subunits provided herein may also function as sodium channels. Second, the isolated human σ 1 H subunit lacks amino acid residues that are generally known to be critical (e.g ., see De Waard et al. ( 1 996) FEBS Letters 380:272-276; Pragnell et al. ( 1 994) Nature 368:67-70) for the interaction between α subunits and the β subunits. There are at least thirteen residues located in this intracellular loop between transmembrane Domains I and II that form a motif that is highly conserved among α subunits, such as σ 1 A -α 1 E described herein (see, also Pragnell et al. ( 1 994) Nature 368:67-70). In particular, this loop lacks the α interaction domain (AID) involved in binding the β subunit. Also absent from this region is the Gβy binding motif, GlnXXGIuArg, originally identified in adenylyl cyclase 2 and found in the non-L-type, HVA α subunits. An identical sequence occurs, however, within the ll-lll intracellular loop of the σ 1H sequence, suggesting a possible interaction of Gβy in this region. The σ 1 H subunit also contains differences in the determinants of ion selectivity found in the S5-S6 linkers of HVA channels. In the S5-S6 pore loops of domain III and IV, the glutamate residues that play a critical role in Ca + selectivity and ion permeation are replaced by aspartate residues. Third, the human σ 1 H subunit has another notably long extracellular loop in Domain I located between IS5 and IS6. This extracellular loop ranges from 249 to 270 nucleotide residues in other human α subunits whereas the human σ 1 H subunit has 426 nucleotide residues. Other distinguishing features may be ascertained and have been ascertained by expressing the subunit in cells as described herein. The nucleic acid encoding an σ 1 H subunit can be used to screen appropriate libraries, particularly mammalian libraries, and more particularly mammalian libraries from tissues or cells that exhibit T-type channel activity. The encoded subunit can be identified by the above- noted distinguishing properties. Nucleic acid probes from the σ 1 H l - encoding clone was used to identify and isolate clones encoding a second variant, designated σ 1 H 2 , which has a 957 bp deletion relative to σ 1 H . j . The σ 1 H subunit forms a functional channel in two different expression systems without the addition of exogenous a 2 δ and β subunits. The absence of a β subunit interaction site within the l-ll loop of the σ 1 H sequence is consistent with the report that β subunit depletion with antisense oligonucleotides in nodosus ganglia has no effect on T- type currents in that region. In addition, none of the known β subunits in HEK293 cells were detected by western analysis using β subunit-specific antisera, indicating that the previously cloned β subunits may not play a role in the formation of LVA Ca 2 + channels containing -r,H. Oόcytes and HEK293 cells express an endogenous a 2 δ subunit and that TT cells, the source of the σ 1 H subunits described here, express relatively high amounts of a 2 δ protein. Consequently, it is possible that σ 1 H -containing channels expressed, contain a 2 ό subunit, and that the a 2 δ subunit is a component of native σ 1H -containing channels. Distribution of σ 1 H transcripts Northern blots containing human mRNA from several neuronal and nonneuronal tissues were probed with labeled fragments generated from the full-length σ 1 H cDNA. A single transcript of ~ 8.5 kb is present in all tissues examined, which included heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas. Neuronal tissues included, cerebellum, cerebral cortex, medulla, spinal cord, occipital lope, frontal lobe, temporal lobe, putamen, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus and thalamus. In nonneuronal tissues, the highest expression levels are found in the kidney, liver, and heart. In the brain, the σ 1 H transcript is most abundant in the amygdala, caudate nucleus, and putamen. Identification and isolation of DNA encoding other a Λ human calcium channel subunit types and subtypes DNA encoding additional a subunits can be isolated and identified using the DNA provided herein as described for the σ 1 A , σ 1 B , σ l c , σ 1 D , σ 1 E and σ 1 H subunits or using other methods known to those of skill in the art. In particular, the DNA provided herein may be used to screen appropriate libraries to isolate related DNA. Full-length clones can be constructed using methods, such as those described herein, and the resulting subunits characterized by comparison of their sequences and electrophysiological and pharmacological properties with the subunits exemplified herein. A number of voltage-dependent calcium channel a subunit genes, which are expressed in the human CNS and in other tissues, have been identified and have been designated as σ 1 A , σ 1 B (or VDCC IV), σ 1 c (or VDCC II), σ 1 D (or VDCC III), σ 1 E and σ 1 H . DNA, isolated from a human DNA libraries that encodes each of the subunit types has been isolated. DNA encoding subtypes of each of the types, which arise as splice variants are also provided. Subtypes are herein designated, for example, as σ 1 B 1 , σ 1 B . 2 . The σ 1 H subunit is of particular interest herein The a subunit types A, B, C, D, E and F of voltage-dependent calcium channels, and subtypes thereof, differ with respect to sensitivity to known classes of calcium channel agonists and antagonists, such as DHPs, phenylalkylamines, omega conotoxins (ω-CgTx), the funnel web spider toxin ω-Aga-IV, pyrazonoylguanidines and or in other physical and structural properties. These subunit types also appear to differ in the holding potential and in the kinetics of currents produced upon depolarization of cell membranes containing calcium channels that include different types of σ, subunits. DNA that encodes an σ, subunit that binds to at least one compound selected from among dihydropyridines, phenylalkylamines, ω- CgTx, components of funnel web spider toxin, and pyrazonoylguanidines is provided. For example, the σ 1 B subunit provided herein appears to specifically interact with ω-CgTx in N-type channels, and the σ 1 D subunit provided herein specifically interacts with DHPs in L-type channels. Antibodies Antibodies, monoclonal or polyclonal, specific for calcium channel subunit subtypes or for calcium channel types can be prepared employing standard techniques, known to those of skill in the art, using the subunit proteins or portions thereof as antigens. Anti-peptide and anti-fusion protein antibodies can be used (see, for example, Bahouth et al. ( 1 991 ) Trends Pharmacol. Sci. 12:338-343; Current Protocols in Molecular Biology (Ausubel et al., eds.) John Wiley and Sons, New York ( 1 984)) Factors to consider in selecting portions of the calcium channel subunits for use as immunogens (as either a synthetic peptide or a recombinantly produced bacterial fusion protein) include antigenicity accessibility (i.e., extracellular and cytoplasmic domains), uniqueness to the particular subunit, and other factors known to those of skill in this art. Antibodies have therapeutic uses and also use in diagnostic assays. The availability of subunit-specific antibodies makes possible the application of the technique of immunohistochemistry to monitor the distribution and expression density of various subunits (e.g., in normal vs diseased brain tissue) . Such antibodies could also be employed in diagnostic, such as LES diagnosis, and therapeutic applications, such as using antibodies that modulate activities of calcium channels. The antibodies can be administered to a subject employing standard methods, such as, for example, by intraperitoneal, intramuscular, intravenous, or subcutaneous injection, implant or transdermal modes of administration. One of skill in the art can empirically determine dosage forms, treatment regiments, and other parameters, depending on the mode of administration employed. Subunit-specific monoclonal antibodies and polyclonal antisera have been prepared. The regions from which the antigens were derived were identified by comparing the DNA and amino acid sequences of all known a or β subunit subtypes. Regions of least homology, preferably human- derived sequences were selected. The selected regions or fusion proteins containing the selected regions are used as immunogens. Hydrophobicity analyses of residues in selected protein regions and fusion proteins are also performed; regions of high hydrophobicity are avoided . Also, and more importantly, when preparing fusion proteins in bacterial hosts, rare codons are avoided. In particular, inclusion of 3 or more successive rare codons in a selected host is avoided . Numerous antibodies, polyclonal and monoclonal, specific for a or β subunit types or subtypes have been prepared; some of these are listed in the following Table. Exemplary antibodies and peptide antigens that have been used to prepare the antibodies are set forth Table 3: TABLE 3 * GST gene fusion system is avai a le rom Pharmacia; see also, Smith et al . (1988) Gene £7:31. The system provides pGEX plasmids that are designed for inducible, high-level expression of genes or gene fragments as fusions with Schistosoma japonicum GST. Upon expression in a bacterial host, the resulting fusion proteins are purified from bacterial lysates by affinity chromatography. The GST fusion proteins are each specific for the cytoplasmic loop region IIS6-IIS1 , which is a region of low subtype homology for all subtypes, including σ ι c and σ 1 D , for which similar fusions and antisera can be prepared . Using similar methods, antibodies specific for LVA subunits, particularly the σ 1 H subunits provided herein, using, for example, the extended intracellular loops, can be prepared . Such antibodies will have use in diagnostic assays for disorders in which LVA calcium channels are implicated. Preparation of recombinant eukaryotic cells containing DNA encoding heterologous calcium channel subunits DNA encoding one or more of the calcium channel subunits or a portion of a calcium channel subunit may be introduced into a host cell for expression or replication of the DNA. Such DNA may be introduced using methods described in the following examples or using other procedures well known to those skilled in the art. Incorporation of cloned DNA into a suitable expression vector, transfection of eukaryotic cells with a plasmid vector or a combination of plasmid vectors, each encoding one or more distinct genes or with linear DNA, and selection of transfected cells are also well known in the art (see, e.g. , Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press). Cloned full-length nucleic acid encoding any of the subunits of a calcium channel may be introduced into a plasmid vector for expression in a eukaryotic cell. Such nucleic acid may be genomic DNA or cDNA or RNA. Presently preferred cells are those containing heterologous DNA encoding an σ 1 H subunit. Host cells may be transfected with one or a combination of the plasmids, each of which encodes at least one calcium channel subunit. Alternatively, host cells may be transfected with linear DNA using methods well known to those of skill in the art. While the DNA provided herein may be expressed in any eukaryotic cell, including yeast cells such as P. pastoris (see, e.g. , Cregg et al. ( 1 987) Bio/Technology 5:479), mammalian expression systems for expression of the DNA encoding the human calcium channel subunits provided herein are preferred. The heterologous DNA may be introduced by any method known to those of skill in the art, such as transfection with a vector encoding the heterologous DNA. Particularly preferred vectors for transfection of mammalian cells are the pSV2dhfr expression vectors, which contain the SV40 early promoter, mouse dhfr gene, SV40 polyadenylation and splice sites and sequences necessary for maintaining the vector in bacteria, cytomegalovirus (CMV) promoter-based vectors such as pCDNAI , or pcDNA-amp and MMTV promoter-based vectors. The vector pcDNAI is a eukaryotic expression vector containing a cytomegalovirus (CMV) promoter which is a constitutive promoter recognized by mammalian host cell RNA polymerase II. DNA encoding the human calcium channel subunits has been inserted in the vector pCDNAI at a position immediately following the CMV promoter. The vector pCDNAI is presently preferred and has been used to express the σ 1 H subunits in mammalian cells. Stably or transiently transfected mammalian cells may be prepared by methods known in the art by transfecting cells with an expression vector having a selectable marker gene such as the gene for thymidine kinase, dihydrofolate reductase, neomycin resistance or the like, and, for transient transfection, growing the transfected cells under conditions selective for cells expressing the marker gene. Functional voltage- dependent calcium channels have been produced in HEK 293 cells transfected with a derivative of the vector pCDNAI that contains DNA encoding a human calcium channel subunit. The heterologous DNA may be maintained in the cell as an episomal element or may be integrated into chromosomal DNA of the cell. The resulting recombinant cells may then be cultured or subcultured (or passaged, in the case of mammalian cells) from such a culture or a subculture thereof. Methods for transfection, injection and culturing recombinant cells are known to the skilled artisan. Eukaryotic cells in which DNA or RNA may be introduced, include any cells that are transfectable by such DNA or RNA or into which such DNA may be injected. Virtually any eukaryotic cell can serve as a vehicle for heterologous DNA. Preferred cells are those that can also express the DNA and RNA and most preferred cells are those that can form recombinant or heterologous calcium channels that include one or more subunits encoded by the heterologous DNA. Such cells may be identified empirically or selected from among those known to be readily transfected or injected . Preferred cells for introducing DNA include those that can be transiently or stably transfected and include, but are not limited to, cells of mammalian origin, such as COS cells, mouse L cells, CHO cells, human embryonic kidney cells, African green monkey cells and other such cells known to those of skill in the art, amphibian cells, such as Xenopus laevis oόcytes, or those of yeast such as Saccharomyces cerevisiae or Pichia pastoris. Preferred cells for expressing injected RNA transcripts or cDNA include Xenopus laevis oδcytes. Cells that are preferred for transfection of DNA are those that can be readily and efficiently transfected. Such cells are known to those of skill in the art or may be empirically identified. Preferred cells include DG44 cells and HEK 293 cells, particularly HEK 293 cells that can be frozen in liquid nitrogen and then thawed and regrown. Such HEK 293 cells are described, for example in U.S. Patent No. 5,024,939 to Gorman (see, also Stillman et al. ( 1 985) Mol. Cell. Biol. 5:2051 -2060) . The cells may be used as vehicles for replicating heterologous DNA introduced therein or for expressing the heterologous DNA introduced therein. In certain embodiments, the cells are used as vehicles for expressing the heterologous DNA as a means to produce substantially pure human calcium channel subunits or heterologous calcium channels. Host cells containing the heterologous DNA may be cultured under conditions whereby the calcium channels are expressed. The calcium channel subunits may be purified using protein purification methods known to those of skill in the art. For example, antibodies, such as those provided herein, that specifically bind to one or more of the subunits may be used for affinity purification of the subunit or calcium channels containing the subunits. Substantially pure subunits of a human calcium channel σ, subunits of a human calcium channel, α 2 subunits of a human calcium channel, β subunits of a human calcium channel and y subunits of a human calcium channel are provided. Substantially pure isolated calcium channels that contain at least one of the human calcium channel subunits are also provided. Substantially pure calcium channels that contain a mixture of one or more subunits encoded by the host cell and one or more subunits encoded by heterologous DNA or RNA that has been introduced into the cell are also provided . Substantially pure subtype- or tissue-type specific calcium channels are also provided. In one embodiment, eukaryotic cells that contain heterologous DNA encoding at least one of a subunit of a calcium channel, preferably an σ 1 H subunit, that express the σ 1 H subunit and form functional homomeric human σ 1 H -containing calcium channels are provided . These cells may be used to screen for compounds that modulate the activity of T-type channels and LVA type calcium channels. In other embodiments, eukaryotic cells that contain heterologous DNA encoding at least one of an a^ subunit of a human calcium channel, an a 2 subunit of a human calcium channel, a β subunit of a human calcium channel and a y subunit of a human calcium channel are provided . In accordance with one preferred embodiment, the heterologous DNA is expressed in the eukaryotic cell and preferably encodes a human calcium channel a subunit. Expression of heterologous calcium channels: electrophysiology and pharmacology The a W subunit-encoding DNA was transiently expressed in HEK203 cells and associated with expression of an a λ H protein of approximately 260kDa a W , as identified by SDS-PAGE/Western blot analysis. Ba 2 + or Ca 2 + currents recorded from HEK293 cells transiently expressing σ 1 H ., channels, and found to exhibit biophysical and pharmacological properties characteristic of low-voltage activated, i.e., T- type, calcium channel currents. Similar results were obtained in Xenopus oocytes expressing σ 1H -τ Electrophysiological methods for measuring calcium channel activity are known to those of skill in the art and are exemplified herein. Any such methods may be used in order to detect the formation of functional calcium channels and to characterize the kinetics and other characteristics of the resulting currents. Pharmacological studies may be combined with the electrophysiological measurements in order to further characterize the calcium channels. With respect to measurement of the activity of functional heterologous calcium channels, preferably, endogenous ion channel activity and, if desired, heterologous channel activity of channels that do not contain the desired subunits, of a host cell can be inhibited to a significant extent by chemical, pharmacological and electrophysiological means, including the use of differential holding potential, to increase the S/N ratio of the measured heterologous calcium channel activity. Thus, various combinations of subunits encoded by the DNA provided herein are introduced into eukaryotic cells. The resulting cells can be examined to ascertain whether functional channels are expressed and to determine the properties of the channels. In particularly preferred aspects, the eukaryotic cell which contains the heterologous DNA expresses it and forms a recombinant functional calcium channel activity. In more preferred aspects, the recombinant calcium channel activity is readily detectable because it is a type that is absent from the untransfected host cell or is of a magnitude and/or pharmacological properties or exhibits biophysical properties not exhibited in the untransfected cell. The eukaryotic cells can be transfected with various combinations of the subunit subtypes provided herein. The resulting cells will provide a uniform population of calcium channels for study of calcium channel activity and for use in the drug screening assays provided herein. Experiments that have been performed have demonstrated the inadequacy of prior classification schemes. Preferred among transfected cells is a recombinant eukaryotic cell with a functional heterologous calcium channel . The recombinant cell can be produced by introduction of and expression of heterologous DNA or RNA transcripts encoding an σ, subunit of a human calcium channel as a homomer, more preferably also expressing, a heterologous DNA encoding a β subunit of a human calcium channel and/or heterologous DNA encoding an a 2 subunit of a human calcium channel. Especially preferred is the expression in such a recombinant cell of each of the a , β and a 2 subunits encoded by such heterologous DNA or RNA transcripts, and optionally expression of heterologous DNA or an RNA transcript encoding a y subunit of a human calcium channel. The functional calcium channels may preferably include at least an α^ subunit and a β subunit of a human calcium channel. Eukaryotic cells expressing these two subunits and also cells expressing additional subunits, have been prepared by transfection of DNA and by injection of RNA transcripts. Such cells have exhibited voltage-dependent calcium channel activity attributable to calcium channels that contain one or more of the heterologous human calcium channel subunits. For example, eukaryotic cells expressing heterologous calcium channels containing an a 2 subunit in addition to the a subunit and a β subunit have been shown to exhibit increased calcium selective ion flow across the cellular membrane in response to depolarization, indicating that the a 2 subunit may potentiate calcium channel function. Cells that have been co-transfected with increasing ratios of a 2 to α, and the activity of the resulting calcium channels has been measured. The results indicate that increasing the amount of a 2 - encoding DNA relative to the other transfected subunits increases calcium channel activity. Eukaryotic cells that express heterologous calcium channels containing a human a subunit as a homomer, particularly the σ 1 H subunit, or at least a human a^ subunit and optionally an a 2 δ subunit and/or a human β subunit are preferred. Eukaryotic cells transformed with a composition containing DNA or an RNA transcript that encodes an σ, subunit alone or in combination with a β and/or an a 2 subunit may be used to produce cells that express functional calcium channels. Since recombinant cells expressing human calcium channels containing all of the human subunits encoded by the heterologous DNA or RNA are especially preferred, it is desirable to inject or transfect such host cells with a sufficient concentration of the subunit-encoding nucleic acids to form calcium channels that contain the human subunits encoded by heterologous DNA or RNA. The precise amounts and ratios of DNA or RNA encoding the subunits may be empirically determined and optimized for a particular combination of subunits, cells and assay conditions. In particular, mammalian cells have been transiently and stably tranfected with DNA encoding one or more human calcium channel subunits. Such cells express heterologous calcium channels that exhibit pharmacological and electrophysiological properties that can be ascribed to human calcium channels. Such cells, however, represent homogeneous populations and the pharmacological and electrophysiological data provides insights into human calcium channel activity heretofore unattainable. For example, HEK cells that have been transiently transfected with DNA encoding the σ 1 E , σ 2b , and ? 1 3 subunits. The resulting cells transiently express these subunits, which form calcium channels that have properties that appear to be a pharmacologically distinct class of voltage-activated calcium channels distinct from those of L-, N-, T- and P-type channels. The observed σ 1 E currents were insensitive to drugs and toxins previously used to define other classes of voltage-activated calcium channels. HEK cells that have been transiently transfected with DNA encoding σ 1 B . σ 2b , and β _ 2 express heterologous calcium channels that exhibit sensitivity to cc-conotoxin and currents typical of N-type channels. It has been found that alteration of the molar ratios of σ 1 B . 1 f σ 2b and β _ 2 introduced into the cells to achieve equivalent mRNA levels significantly increased the number of receptors per cell, the current density, and affected the K d for ω-conotoxin. The electrophysiological properties of these channels produced from σ 1 B 1 , σ 2b , and β _ 2 was compared with those of channels produced by transiently transfecting HEK cells with DNA encoding σ 1 B .ι , σ 2b and / -? 1 3 . The channels exhibited similar voltage dependence of activation, substantially identical voltage dependence, similar kinetics of activation and tail currents that could be fit by a single exponential. The voltage dependence of the kinetics of inactivation was significantly different at all voltages examined. In certain embodiments, the eukaryotic cell with a heterologous calcium channel is produced by introducing into the cell a first composition, which contains at least one RNA transcript that is translated in the cell into a subunit of a human calcium channel. In preferred embodiments, the subunits that are translated include an a subunit of a human calcium channel. More preferably, the composition that is introduced contains an RNA transcript which encodes an a subunit of a human calcium channel and also contains (1 ) an RNA transcript which encodes a β subunit of a human calcium channel and/or (2) an RNA transcript which encodes an a 2 subunit of a human calcium channel. Especially preferred is the introduction of RNA encoding an a , a β and an a 2 human calcium channel subunit, and, optionally, a y subunit of a human calcium channel. Methods for in vitro transcription of a cloned DNA and injection of the resulting RNA into eukaryotic cells are well known in the art. Transcripts of any of the full-length DNA encoding any of the subunits of a human calcium channel may be injected alone or in combination with other transcripts into eukaryotic cells for expression in the cells. Amphibian oόcytes are particularly preferred for expression of in vitro transcripts of the human calcium channel subunit cDNA clones provided herein. Amphibian oocytes that express functional heterologous calcium channels have been produced by this method. Pharmacological and electrophysiological properties As described in the examples, nucleic acid encoding σ 1 H .ι and nucleic acid encoding σ 1 H 2 has been expressed in mammalian cells and in amphibian oόcytes. Electrophyisological and pharmacological properties have been studied. The biophysical properties of recombinant human σ 1 H 2+ channels expressed in HEK293 cells and Xenopus oocytes are in good agreement, indicating that the biophysical properties of recombinant human σ 1 H channels are independent of the expression system. Several biophysical characteristics support the conclusion that the human σ 1 H subunit is the pore-forming a subunit of a T-type channel. The rates of activation, inactivation, and deactivation and the single-channel conductance of σ 1 H -containing channels are within the ranges described for T-type channels. The conductance value of 9 pS measured in this study is near the value determined for rat σ 1 G -containing channels and is significantly lower than those determined for recombinant HVA channels. In addition, σ 1 H -containing channels conduct Ba2 + and Ca 2 + equally well, consistent with the finding that the conductance of T-type channels for Ba2 + and Ca 2 + is nearly equivalent in most cell types. σ 1 H -containing Ca 2 + channels display a pharmacological profile differing from those of HVA channels. σ 1 H -mediated currents are inhibited by Ni 2 + , amiloride, and mibefradil (Ro 40-5967), agents shown to reduce LVA currents in a number of cell types. In contrast, ethosuximide, an antiepileptic agent that inhibits LVA currents in some cell types, had no effect on α 1 H -mediated currents. Although the L-type Ca 2 + -channel modulators nimodipine and (-)-Bay K 8644 had little effect at a concentration of λμM on α 1 H -containing channels, both compounds produced a marked inhibition at a concentration of 10 μM, consistent with their effects on T-type channels in rat hypothalamic neurons (Akaike et al., 1 989) . In summary, the pharmacological properties of σ 1 H -containing channels described here have many similarities to native T-type channels studied in a variety of cell types. The pharmacological profiles of T-type channels vary considerably between cell types, and no hallmark pharmacological feature of T-type channels has been identified. These results are consistent with the finding herein that multiple σ, subunits are responsible for the pharmacological profiles of a family of LVA, or T-type, channels. Assays and Clinical uses of the cells and calcium channels Assays Assays for identifying compounds that modulate calcium channel activity Among the uses for eukaryotic cells which recombinantly express one or more subunits are assays for determining whether a test compound has calcium channel agonist or antagonist activity. These eukaryotic cells may also be used to select from among known calcium channel agonists and antagonists those exhibiting a particular calcium channel subtype specificity and to thereby select compounds that have potential as disease- or tissue-specific therapeutic agents. In vitro methods for identifying compounds, such as calcium channel agonist and antagonists, that modulate the activity of calcium channels using eukaryotic cells that express heterologous human calcium channels are provided. In particular, the assays use eukaryotic cells that express homomeric or heteromeric human calcium channel subunits encoded by heterologous DNA provided herein, for screening potential calcium channel agonists and antagonists which are specific for human calcium channels and particularly for screening for compounds that are specific for particular human calcium channel subtypes. Such assays may be used in conjunction with methods of rational drug design to select among agonists and antagonists, which differ slightly in structure, those particularly useful for modulating the activity of human calcium channels, and to design or select compounds that exhibit subtype- or tissue- specific calcium channel antagonist and agonist activities. These assays should accurately predict the relative therapeutic efficacy of a compound for the treatment of certain disorders in humans. In addition, since subtype-and tissue-specific calcium channel subunits are provided, cells with tissue- specific or subtype-specific recombinant calcium channels may be prepared and used in assays for identification of human calcium channel tissue- or subtype-specific drugs. Desirably, the host cell for the expression of calcium channel subunits does not produce endogenous calcium channel subunits of the type or in an amount that substantially interferes with the detection of heterologous calcium channel subunits in ligand binding assays or detection of heterologous calcium channel function, such as generation of calcium current, in functional assays. Also, the host cells preferably should not produce endogenous calcium channels which detectably interact with compounds having, at physiological concentrations (generally nanomolar or picomolar concentrations), affinity for calcium channels that contain one or all of the human calcium channel subunits provided herein. With respect to ligand binding assays for identifying a compound which has affinity for calcium channels, cells are employed which express, preferably, at least a heterologous α 1 subunit. Transfected eukaryotic cells which express at least an a subunit may be used to determine the ability of a test compound to specifically bind to heterologous calcium channels by, for example, evaluating the ability of the test compound to inhibit the interaction of a labeled compound known to specifically interact with calcium channels. Such ligand binding assays may be performed on intact transfected cells or membranes prepared therefrom. The capacity of a test compound to bind to or otherwise interact with membranes that contain heterologous calcium channels or subunits thereof, preferably σ 1 H subunit-containing calcium channels, may be determined by using any appropriate method, such as competitive binding analysis, such as Scatchard plots, in which the binding capacity of such membranes is determined in the presence and absence of one or more concentrations of a compound having known affinity for the calcium channel. Where necessary, the results may be compared to a control experiment designed in accordance with methods known to those of skill in the art. For example, as a negative control, the results may be compared to those of assays of an identically treated membrane preparation from host cells which have not been transfected with one or more subunit-encoding nucleic acids. The assays involve contacting the cell membrane of a recombinant eukaryotic cell which expresses at least one subunit of a human calcium channel, preferably at least an a subunit of a human calcium channel, with a test compound and measuring the ability of the test compound to specifically bind to the membrane or alter or modulate the activity of a heterologous calcium channel on the membrane. In preferred embodiments, the assay uses a recombinant cell that has a calcium channel containing an σ, subunit of a human calcium channel. In other preferred embodiments, the assay uses a recombinant cell that has a calcium channel containing an a subunit of a human calcium channel in combination with a β subunit of a human calcium channel and/or an a 2 subunit of a human calcium channel. Recombinant cells expressing heterologous calcium channels containing each of the σ, and optionally a β and/or a 2 human subunits, and, optionally, a y subunit of a human calcium channel are especially preferred for use in such assays. In certain embodiments, the assays for identifying compounds that modulate calcium channel activity are practiced by measuring the calcium channel activity of a eukaryotic cell having a heterologous, functional calcium channel when such cell is exposed to a solution containing the test compound and a calcium channel-selective ion and comparing the measured calcium channel activity to the calcium channel activity of the same cell or a substantially identical control cell in a solution not containing the test compound. The cell is maintained in a solution having a concentration of calcium channel-selective ions sufficient to provide an inward current when the channels open. Recombinant cells expressing calcium channels that include each of the σ 1 # β and a 2 human subunits, and, optionally, a y subunit of a human calcium channel, are especially preferred for use in such assays. Methods for practicing such assays are known to those of skill in the art. For example, for similar methods applied with Xenopus laevis oόcytes and acetylcholine receptors, see, Mishina et al. (( 1 985) Nature 373:364) and, with such oόcytes and sodium channels (see, Noda et al. ( 1 986) Nature 322:826-828) . For similar studies which have been carried out with the acetylcholine receptor, see, e.g. , Claudio et al. (( 1 987) Science 235: 1 688-1 694) . Transcription based assays are also contemplated herein. Functional recombinant or heterologous calcium channels may be identified by any method known to those of skill in the art. For example, electrophysiological procedures for measuring the current across an ion- selective membrane of a cell, which are well known, may be used. The amount and duration of the flow of calcium-selective ions through heterologous calcium channels of a recombinant cell containing DNA encoding one or more of the subunits provided herein has been measured using electrophysiological recordings using a two electrode and the whole-cell patch clamp techniques. In order to improve the sensitivity of the assays, known methods can be used to eliminate or reduce non- calcium currents and calcium currents resulting from endogenous calcium channels, when measuring calcium currents through recombinant channels. For example, the DHP Bay K 8644 specifically enhances L-type calcium channel function by increasing the duration of the open state of the channels (see, e.g. , Hess, J.B., et al. ( 1 984) Nature 37 7 :538-544) . Prolonged opening of the channels results in calcium currents of increased magnitude and duration. Tail currents can be observed upon repolarization of the cell membrane after activation of ion channels by a depolarizing voltage command. The opened channels require a finite time to close or "deactivate" upon repolarization, and the current that flows through the channels during this period is referred to as a tail current. Because Bay K 8644 prolongs opening events in calcium channels, it tends to prolong these tail currents and make them more pronounced. In practicing these assays, stably or transiently transfected cells or injected cells that express voltage-dependent human calcium channels containing one or more of the subunits of a human calcium channel desirably may be used in assays to identify agents, such as calcium channel agonists and antagonists, that modulate calcium channel activity. Functionally testing the activity of test compounds, including compounds having unknown activity, for calcium channel agonist or antagonist activity to determine if the test compound potentiates, inhibits or otherwise alters the flow of calcium ions or other ions through a human calcium channel can be accomplished by (a) maintaining a eukaryotic cell which is transfected or injected to express a heterologous functional calcium channel capable of regulating the flow of calcium channel- selective ions into the cell in a medium containing calcium channel- selective ions (i) in the presence of and (ii) in the absence of a test compound; (b) maintaining the cell under conditions such that the heterologous calcium channels are substantially closed and endogenous calcium channels of the cell are substantially inhibited (c) depolarizing the membrane of the cell maintained in step (b) to an extent and for an amount of time sufficient to cause (preferably, substantially only) the heterologous calcium channels to become permeable to the calcium channel-selective ions; and (d) comparing the amount and duration of current flow into the cell in the presence of the test compound to that of the current flow into the cell, or a substantially similar cell, in the absence of the test compound. The assays thus use cells, provided herein, that express heterologous functional calcium channels and measure functionally, such as electrophysiologically, the ability of a test compound to potentiate, antagonize or otherwise modulate the magnitude and duration of the flow of calcium channel-selective ions, such as Ca 2 + or Ba 2 + , through the heterologous functional channel. The amount of current which flows through the recombinant calcium channels of a cell may be determined directly, such as electrophysiologically, or by monitoring an independent reaction which occurs intracellularly and which is directly influenced in a calcium (or other) ion dependent manner. Any method for assessing the activity of a calcium channel may be used in conjunction with the cells and assays provided herein. For example, in one embodiment of the method for testing a compound for its ability to modulate calcium channel activity, the amount of current is measured by its modulation of a reaction which is sensitive to calcium channel-selective ions and uses a eukaryotic cell which expresses a heterologous calcium channel and also contains a transcriptional control element operatively linked for expression to a structural gene that encodes an indicator protein. The transcriptional control element used for transcription of the indicator gene is responsive in the cell to a calcium channel-selective ion, such as Ca 2 + and Ba 2 + . The details of such transcriptional based assays are described in commonly owned PCT International Patent Application No. PCT/US91 /5625, filed August 7, 1 991 , which claims priority to copending commonly owned allowed U.S. Application Serial No. 07/563,751 , filed August 7, 1 990; see also, commonly owned published PCT International Patent Application PCT US92/1 1090, which corresponds to co-pending U.S. Applications Serial Nos. 08/229, 1 50 and 08/244,985. The contents of these applications are herein incorporated by reference thereto. Biophysical and pharmacological properties of 1H subunits HEK cells were transfected with DNA and oόcytes injected wiht nucleic acid provided herein. The cell expressed calcium channels, which were then characterized electrophysiologically and pharmacologically. These results are described in the examples. Both splice variants formed calcium channels that exhibit properties associated with T-type channels. Variant-specific properties were observed. These observed differences in the amino acid sequences of a λ H _ι and σ 1H . 2 will result in marked differences in susceptibility of these receptors to cellular regulation, particularly since the observed region of sequence divergence resides in the cytosolic linker region between domains I and II and the analogous sequence region in high-voltage activated calcium channels has been implicated in binding of cytosolic regulatory proteins. Observed differences in biophysical properties of α 1 H . ! and α 1 H _ 2 are also likely indicative of differences in the sensitivity of these two different channel subunits to pharmaceutical compounds. Thus, it seems likely that low-voltage activated calcium channels containing either the σ 1 H l or the σ 1 H 2 subunit will be subject to different regulatory controls, and different profiles of susceptibility to pharmaceutical compounds. For example, amiloride blocks the T-type current in neuroblastoma cells with an IC 50 of ~ 50 μM, whereas in hippocampal neurons 300 μM amiloride reduces the T-type current by only 40% . In this respect, each a different σ 1 H channel is a separate screening target for development of pharmaceutical drug compounds. Differential effects of drugs on different neural cells and in different neural tissues can be understood based on different patterns of expression of α 1H .., and/or σ 1 H . 2 in vivo and will provide a means to identify drugs specific for each subtype and associated disorders or conditions. The observed sequence variation in σ 1 H subunits explains observed pharmacological variability of T-type calcium channels in different native tissues, providing a useful tool to identify where the respective Gr 1 h and α 1 H . 2 subunit is expressed to use screening assays to identify targeted therapeutic drug candidates. Differences in a . and α 1 H . 2 functionality and expression in different tissues provides basis for using recombinant cells expressing calcium channels having either the α^., or α 1 H . 2 subunit. Agonists and antagonists capable of differentially affecting calcium channels containing these two different subunits should be useful for targeting therapeutic intervention into selected neural locations, e.g., to cardiovascular neurons an cardiac pacemaker neurons expressing σ 1 H 2 . Calcium channels formed from σ 1 H subunits open at small changes in membrane potential, but only allow moderate Ca 2 + influx before closing. By allowing moderate influx of divalent ions the σ 1 H containing channels are likely to: (i) participate in pathways triggering changes in gene expression in response to subtle change sin membrane potential difference, i.e., in neuronal and non-neuronal cell types (e.g., in activation of immune cells such as T-cells, in activation of kidney and liver cells in response to metabolic changes; (ii) exert subtle controls over the overall excitability or accessibility of neurons to synaptic transmission, such as in determining which neurons will respond to stimulae, and to what extent, such as in peripheral neurons and ganglia; (iii) determine the extent of neural responses to stimulae such as chronic pain; (iv) regulate the sensitivity of neurons in critical neural centers so that neuronal cells in these centers are protected from the adverse effects associated with excessive bursts of firing (e.g., in the cardiac pacemaker); (v) act to set the steady state pattern of inactivation of neurons in different regions of the brain, (e.g., in response to sleep, sex, emotion, depression, fatigue and the other stimulae or conditions) . Electrophysiology of cells that express channels containing the σ 1H 1 subunit Expression of recombinant σ 1H 1 channels Following transient transfection of HEK293 cells with a DNA encoding the σ 1 H subunit, Ba 2 + currents that were rapidly activating and inactivating were observed. Ba 2 + currents (1 5 elicited by step depolarizations to various test potentials from a holding potential of -90- mV were measured. Currents were activated at a test potential of -50 mV, peaked between -20 and -10 mV, and reversed at a membrane potential more positive that + 60 mV. Similar results were obtained with Ca 2 + ( 1 5 mM) as the charge carrier. One hallmark of LVA channels is their slow rate of deactivation, which is reflected in a show decay of tail currents. The time constant of this decay is ~ 10-fold slower for LVA channels (2-1 2 ms) than for HVA channels < 300 μs. A slow decay of a H _ mediated tail currents over a period of — 1 5 ms was observed. In contrast to the monoexponential decay of the tail currents reported for many native T-type Ca 2 + channels, tail currents from σ 1 H .., channels showed a biexponential decay. At a test potential of -20 mV, the decay rate of the slow component, comprising 88.1 ± 33.8% of the total current, was 2.1 ± 1 .06 ms (n = 6), which is similar to those observed in native T-type Ca 2 + channels. The decay rate of the faster component was 0.64 ± 0.21 ms (n = 6). Whole-cell patch clamp recordings were performed on HEK293 cells transiently expressing the human σ 1 H 1 subunit. Step-depolarizations elicited inward Ba 2 + currents that activate slowly and inactivate rapidly (2.8 ± 0.6 and 1 6.9 ± 5.3 ms, at -20 mV) . The activation curve of σ 1 H ., is shifted to the left (V1 /2:-29.5 mV) compared to HVA ca 2 + channels. The tails currents of σ 1H . containing channels decay slowly (r1 , τ2 ± 1 .0, 0.6, ± 0.2 ms) . The permeability for Ba 2 + and Ca 2 + was virtually identical. The single channel conductance, determined with 1 10 mM ba 2 + as charge carrier, is 9pS. The voltage dependence of activation of a H containing Ca 2 + channels was determined from tail-current analysis. Normalized tail- current amplitudes were plotted as a function of test potential and revealed a biphasic activation curve that was well fitted by the sum of two Boltzmann functions (Figure 1 ) . The potentials for half-maximal activation of the individual Boltzmann terms were as follows: V, Λ A : -25.1 ± 3 3.0 mV; and V, /2 B : + 25.5 ± 3 9.9 mV (n = 1 1 ) . A value similar to V, /2 A has been reported previously for voltage dependence of activation of T-type CA 2 + channels in the human TT cell line (-27 mV) . The value of the second Boltzmann term V, Λ B is somewhat similar to that reported for HVA Ca 2 + channels. Using a similar protocol, tail currents of HVA Ca 2+ channels decay with time constants of < 300 μs, whereas with σ 1 H the most prominent at test potentials close to V, /2 B . The availability of 1 H containing Ca 2 + channels for opening was dependent on the membrane for potential as shown in Fig. 1 . The potential for half-maximal steady- state inactivation (V, Λ ) was - 63.2 ± 2.0 mV (n = 9). The rapid inactivation of σ 1 H Ca 2 + channels was strongly voltage- dependent. The current decay was best described with an exponential function with time constants ranging from 42.2 ± 7.8 to 8.8 ± 3.8 ms at membrane potentials between -50 and + 30 mV (n = 6; data not show) . Activation kinetics of σ 1H Ca 2 + channels were also voltage- dependent with time constants ranging from 9.9 ± 4.7 to 0.9 ± 0.3 ms for membrane potentials between -50 and + 30 mV (n = 8; data not shown) . σ 1 H Ca 2 + channels inactivated completely during the 1 50-ms depolarization. Recovery from inactivation occurred within a period of ~ 3 s with a fast component (r = 37 ± 9 ms; 16.5 ± 4.6% of all channels) and a slow component (r = 37 ± 61 ms; 78 ± 8.5% of all channels; n = 3; data not shown) . To confirm the biophysical properties of recombinant σ 1 H channels observed in whole-cell recordings from HEK293 cells, the functional expression of σ 1 H in Xenopus oόcytes was tested. Substantial currents ( < 1 μA) after injection of σ 1 H transcripts alone was observed. The activation and inactivation kinetics, as well as the steady-state inactivation properties, were similar to those obtained in HEK293 cells (see EXAMPLES) . Single-channel properties of σ 1 H Ca 2 + channels in HEK293 cells were determined in cell-attached recordings with 1 1 0 mM Ba 2 + as the charge carrier. Single-channel recordings at a test potential of -30 mV from a patch that contains at least three σ 1 H showed that channel openings occurred in bursts and were clustered mainly in the first third of the 100- ms depolarizing pulse, especially with stronger depolarizations. Occasionally, channel activity was spread throughout the entire sweep. The time course of the ensemble-averaged current recorded at -30mV in 1 1 0 mM Ba 2 + was similar to the σ 1 H whole-cell Ba 2 + current recorded at - 40 mV in 1 5 mM Ba 2 + . The currents were compared at different potentials to compensate for the shift in the activation curve to more positive potentials due to the increase in divalent concentration. The unitary current-voltage relationship yielded a unitary slope conductance of 9.06 ± 0.22 pS (n = 4). Summary of Electrophysiologic Characteristics The biophysical properties of calcium channels containing the human σ 1 H subunit were evaluated. Whole cell recordings from transiently transfected HEK293 cells indicate that the current-voltage relationship, permeability to Ca 2 + and Ba 2 + , kinetics of activation, and single channel conductance of calcium channels containing σ 1 H subunits were similar to those of native T-type calcium channels in tissues. Tail currents from A 1 H channels showed a bi-exponential decay, exhibiting a fast and a slower component. At very negative membrane potentials (-1 50 to -100 mV) the fast component (r: 200-450 μs) dominated the inactivation process, while at depolarizing potentials > -50 mV the slower component (2-3 ms) dominated. At the resting membrane potential, i.e., <-80 mV, both components contribute equally. Pharmacological properties The pharmacological properties of σ 1 H -containing calcium channels were also consistent with those observed for native T-type calcium channels. Interestingly, the sensitivity of σm^-containing calcium channels to Cd 2 + or Amiloride was about 10-fold lower when expressed in HEK293 cells than when expressed in Xenopus oόcytes. The data indicate that human σ 1 H calcium channel subunits have properties consistent with that of native T-type calcium channels and, as such, σ 1 H represent a member in the rapidly growing family of low-voltage activated calcium channels. Assays for diagnosis of LVA-caicium channel mediated disorders and clinical applications Clinical applications In relation to therapeutic treatment of various disease states, the availability of DNA encoding human calcium channel subunits permits identification of any alterations in such genes (e.g., mutations) which may correlate with the occurrence of certain disease states. In addition, the creation of animal models of such disease states becomes possible, by specifically introducing such mutations into synthetic DNA fragments that can then be introduced into laboratory animals or in vitro assay systems to determine the effects thereof. Also, genetic screening can be carried out using the nucleotide sequences as probes. Thus, nucleic acid samples from subjects having pathological conditions suspected of involving alteration/modification of any one or more of the calcium channel subunits can be screened with appropriate probes to determine if any abnormalities exist with respect to any of the endogenous calcium channels. Similarly, subjects having a family history of disease states related to calcium channel dysfunction can be screened to determine if they are also predisposed to such disease states. Disorders and for which screening assays can be developed and also for which candidate compounds for treatment of the disorders include, but are not limited to: cardiac treatments, such as myocardial infarct, cardiac arrhythmia, heart failure, and angina pectoris. Identified compounds will be useful in: (a) adjunctive therapies for reestablishing normal heart rate and cardiac output following traumatic injury, heart attack and other heart injuries; (b) treatments of myocardial infarct (Ml) , post-MI and in an acute setting. The compounds may be effective to increase cardiac contractile force, such as that measured by left ventricular enddiastolic pressure, and without changing blood pressure or heart rate. In an acute setting the compounds may be effective to decrease formation of scar tissue, such as that measured by collagen deposition or septal thickness, and without cardiodepressant effects. The identified compounds will be useful for and assays for diagnosis and compound screening will be useful in connection with vascular treatments and hypertension, for identifying compounds useful in regulating vascular smooth muscle tone, including vasodilating or vasoconstricting . Such compounds can be used in (a) treatments for reestablishing blood pressure control, e.g., following traumatic injury, surgery or cardiopulmonary bypass, and in prophylactic treatments designed to minimizing cardiovascular effects of anaesthetic drugs; (b) treatments for improving vascular reflexes and blood pressure control by the autonomic nervous system. Other conditions include urologic, for identifying compounds useful in: (a) treating and restoring renal function following surgery, traumatic injury, uremia and adverse drug reactions; (b) treating bladder dysfunctions; and (c) uremic neuronal toxicity and hypotension in patients on hemodialysis; reproductive conditions, for identifying compounds useful in treating: (a) disorders of sexual function including impotence; and (b) alcoholic impotence (under autonomic control that may be subject to T-channel controls); hepatic, for identifying compounds useful in treating and reducing neuronal toxicity and autonomic nervous system damage resulting from acute over-consumption of alcohol; neurological conditions for identifying compounds useful in treating: (a) epilepsy and diencephalic epilepsy; (b) Parkinson disease; (c) aberrant temperature control, such as abnormalities of shivering and sweat gland secretion and peripheral vascular blood supply; (d) aberrant pituitary and hypothalamic functions including abnormal secretion of noradrenaline, dopamine and other hormones; respiratory conditions, for identifying compounds useful in treating abnormal respiration, such as, post-surgical complications of anesthetics; endocrine disorders for identifying compounds useful in treating aberrant secretion of hormones such as treatments for overproduction of hormones including insulin, thyroxin, and adrenalin. EXAMPLES The following examples are included for illustrative purposes only and are not intended to limit the scope of the invention. EXAMPLE 1 : ISOLATION OF DNA ENCODING THE HUMAN CALCIUM CHANNEL σ^., SUBUNIT Using mRNA and TT cells, a degenerate PCR approach was used to isolate nucleic acid encoding an a subunit. Nucleic acid encoding an a H subunit and nucleic acid encoding a subunit designated as σ 1 H . 2 was isolated. The nucleic acid was introduced into HEK293 cells and Xenopus oόcytes and voltage gated calcium channels were expressed. These channels exhibit pharmacological and electrophyiological properties consistent with native LVA, T-type, channels. A. Materials and Methods Nucleic acid amplification: The following sense strand 20-mer PCR primer, corresponding to nucleotides 1 945-1 964 of DNA encoding a human σ 1 E subunit, was synthesized: AC(A/C/G/T)GTGTT(C/T)CAGATCCTGAC (Primer- 1 ) SEQ ID NO. 4 An antisense 22-nucleotide PCR primer, corresponding to nucleotides 391 9 through 3940 of human σ 1 E , was also synthesized: T(C/T)CCCTTGAAGAGCTG(A/C/G/T)ACCCC (Primer-2) SEQ ID NO. 1 The sense and the antisense primers were used in amplification reactions with cDNA prepared from TT cells and Pfu DNA polymerase (Stratagene Inc., San Diego, CA). Reaction conditions: 95 °C for 5 minutes followed by 5 cycles of 20 seconds each at 95 °C; then 20 seconds at 42°C; 2.5 minutes at 72°C; and, 30 cycles of 20 seconds each at 95 °C followed by 20 seconds at 50°C and finally 2.5 minutes at 72°C. The product of the reaction is referred to herein (below) as "the original PCR products. " A second 5' degenerate oligonucleotide primer was designed corresponding to a portion of the sequence reported for C. elegans, cosmid C54D2 (Genebank accession #U37548), as a portion of that sense strand sequence which aligns with a portion of the human σ 1 E subunit DNA sequence between nucleotide 3598 and 3614. This primer had the following sequence: GA(A/G)ATGATGATGAA(A/G)GT (Primer-3) SEQ ID NO. 10 Primer-3 was used in a nested amplification reaction with the original PCR products and the Primer-2. Isolation and Characterization of the clones: A recombinant cDNA library was constructed in phage vector Λgt1 0 using poly(A) + -selected RNA from the TT cell line. Approximately 1 .5x10° were screened with the PCR fragment under high stringency (hybridization: 50% formamide, 5X SSPE, 5X Denhardts, 0.2% SDS, 200 vg/ml herring sperm DNA for 1 6- 1 8 hrs. at 42°C; wash: 6 washes of 30 minutes each in 0.1 X SSPE, 0.1 % SDS at 65 °C). Northern blot analysis: Multiple tissues were screened in Northern blots using 2μg of poly (A) + RNA per lane (Clontech, Palo Alto, CA) . Blots were probed at high stringency, as described above, with labeled fragments generated from the full-length σ 1 H cDNA, i.e., nucleotide -6 to 7390. Western blot analysis: Cellular membranes (total) were isolated from HEK293 cells expressing different 1 H subunits; membrane proteins were separated by SDS-PAGE; transferred to nitrocellulose; and, blotted using a polyclonal anti-σ 1 H antisera and TBS-T buffer. Blotted proteins were visualized using the Lumiglo reagent kit (KPL, Gaithersburg, MD) according to the manufacturer's instructions. B. RNA isolation Human medullary thyroid carcinoma cells (TT cells; ATCC Accession No. CRL1 803) were grown in DMEM medium supplemented with 10 % fetal calf serum at 37 °C in 5% CO 2 atmosphere and total cytoplasmic RNA was isolated from forty 10 cm plates using a "midi- prep" RNA isolation kit (Qiagen) as per the manufacturer's instructions. The protocol entails the use of the detergent NP40 which lyses the cell membrane under mild conditions such that the nuclear membrane remains intact thereby eliminating incompletely spliced RNA transcripts from the preparation. PolyA + RNA was isolated from total cytoplasmic RNA using two passes over an oligo(dT)-cellulose column. Briefly, 2-3 mg of total cytoplasmic RNA was resuspended in NETS buffer (500 mM NaCI 1 0 mM EDTA, 1 0 mM Tris, pH 7.4, 0.2% SDS) and passed slowly over a column containing 0.5 g of oligo(dT)-cellulose (Collaborative Research) equilibrated in NETS buffer. The column was washed with 30 mis of NETS buffer and polyA + RNA was eluted using about 3 mis of ETS buffer ( 10 mM EDTA, 1 0 mM Tris, pH 7.4, 0.2% SDS) . The ionic strength of the polyA + RNA-containing buffer was adjusted to 500 mM NaCI and passed over a second oligo(dT)-cellulose column essentially as described above. Following elution from the second column, the polyA + RNA was precipitated twice in ethanol and resuspended in H 2 O. C. Library construction Double stranded cDNA (dscDNA) was synthesized according to standard methods (see, e.#. ,Gubler et al. ( 1 985) Gene 25: 263-269; Lapeyre et al. ( 1 985) Gene 37:21 5-220) . Briefly, first strand cDNA synthesis was initiated using TT cell polyA + RNA as a template and using random primers and Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) . The second strand was synthesized using a combination of E coli DNA polymerase, EL coli DNA ligase and RNase H. Regions of single stranded DNA were converted to double-stranded DNA using T4 DNA polymerase generating blunt-ended double stranded fragments. EcoRI restriction endonuclease site adapters: 5' CGTGCACGTCACGCTAG 3' (SEQ ID NO. 2) 3' GCACGTGCAGTGCGATCTTAA 5' (SEQ ID NO. 3) were ligated to the double-stranded cDNA using a standard protocol (see, e.g. , Sambrook et al. ( 1 989) IN: Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Chapter 8). The double-stranded DNA with the fcoRI adapters ligated was purified away from the free or unligated adapters by column chromatography using Sepharose CL-4B resin followed by size selection of the cDNA on a 1 .2% agarose gel. After visualizing the resolved DNA using ethidium bromide, two fractions of cDNA, > 3.5 kb and 1 .0-3.5 kb, were isolated from the gel and inserted into the vector λgt1 0. The ligated Λgt1 0 containing the cDNA insert was packaged into λ phage virions in vitro using the Gigapack III Gold packaging (Stratagene, La Jolla, CA) kit. Using this method, phage libraries of - 1 .5 x 10 6 recombinants for cDNA > 3.5 kb fraction and - 10 x 10 6 recombinants for DNA fraction between 1 .0 and 3.5 kB were obtained. D. Isolation of DNA encoding a portion of human σ 1 calcium channel subunits DNA encoding a small region of human a subunits encoded in TT cells was isolated using degenerate PCR-based amplification (e.g., see Williams et al. ( 1 994) J. Biol. Chem. 269:22347-22357) . These amplified fragments were used to generate DNA probes for the isolation of DNA encoding a full-length human σ 1 H calcium channel subunit. As noted above, two sets of degenerate oligonucleotides were synthesized based on the flanking regions of the ll-lll loop known to share a high degree of sequence identity amongst known human α calcium channel subunits: 1 ) two degenerate oligonucleotides complementary to the regions of the IIS5-IIS6 loop were synthesized as 5' upstream primers (SEQ ID NOs. 4 and 5); and 2) two degenerate oligonucleotides complementary to a portion of the IIIS5 transmembrane segment were synthesized as 3' downstream primers (SEQ ID NOs. 6 and 7) . These degenerate oligonucleotides were used as primer pairs in nested PCR amplification reactions using Pfu DNA polymerase (Stratagene, La Jolla, CA) and reactions were performed according to the manfacturer's instructions. Samples were placed in a commercially available thermocycler (Perkin-Elmer) and the amplification reactions were set as follows: 1 cycle, 5 min @ 95 °C; 5 cycles, 20 sec @ 95 °C/20 sec @ 42 °C/2.5 min @ 72 °C; 30 cycles, 20 sec @ 95 °C/20 sec @ 50 °C/2.5 min @ 72 °C; and 1 cycle, 7 min @ 72 °C. Amplified DNA products were subjected to electrophoresis on an agarose gel and gel purified using standard methods. E. Amplification of DNA encoding a portion of human σ 1 H calcium channel subunit To amplify DNA encoding a portion of the human σ 1 H calcium channel subunit, three degenerate oligonucleotides (SEQ ID NOs. 8-1 0) that share partial complementarity to a region of Domain III were synthesized as 5' primers. This region is encompassed within all of the amplified ^-encoding fragments of Section C above. Two oligonucleotides based on sequences in IIIS2 (SEQ ID NOs. 8 and 1 0) were used as 5' primers in conjunction with the 3 IIS5 transmembrane primers used in the initial PCR reactions (SEQ ID NOs. 6 and 7 to amplify DNA encoding a portion of the human σ 1 H subunit using the amplified products as templates. The amplified DNA products were subcloned into the pCR-Blunt vector (Invitrogen), plasmid DNA was purified from isolated transformants and the DNA sequence of each insert was determined. A 340 bp fragment (SEQ ID NO. 48; nt 4271 to 461 0 of SEQ ID NO. 49) that shares approximately 55-60% sequence identity to known human a calcium channel subunits was identified. This DNA fragment, designated PCR1 , was used as a DNA probe to isolate DNA encoding a human σ 1 H calcium channels subunit. F. Isolation and characterization of individual clones Hybridization and Washing Conditions Hybridization of radiolabelled nucleic acids to immobilized DNA for the purpose of screening cDNA libraries, DNA Southern transfers, or northern transfers was routinely performed in standard hybridization conditions (hybridization: 50% deionized formamide, 200 μg/ml sonicated herring sperm DNA (Cat #223646, Boehringer Mannheim Biochemicals, Indianapolis, IN), 5 x SSPE, 5 x Denhardt's, 42° C; wash :0.2 x SSPE, 0.1 % SDS, 65 ° C) . The recipes for SSPE and Denhardt's and the preparation of deionized formamide are described, for example, in Sambrook et al. ( 1 989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Chapter 8) . In some hybridizations, lower stringency conditions were used in that 10% deionized formamide replaced 50% deionized formamide described for the standard hybridization conditions. The washing conditions for removing the non-specific probe from the filters was either high, medium, or low stringency as described below: 1 ) high stringency: 0.1 x SSPE, 0.1 % SDS, 65 °C 2) medium stringency: 0.2 x SSPE, 0.1 % SDS, 50°C 3) low stringency: 1 .0 x SSPE, 0.1 % SDS, 50°C. It is understood that equivalent stringencies may be achieved using alternative buffers, salts and temperatures. Approximately 1 .5 x 10 5 recombinants of the TT cell phage library containing inserts > 3.5 kb were plated and duplicate lifts prepared from each plate. The lifts were probed with radiolabelled PCR1 using standard hybridization conditions, the filters were washed and approximately 1 00 positive plaques were identified. Initially, 5 positives, Λ 1 .201 -/11 .205, were selected for plaque purification and characterization. Restriction endonuclease digestion of purified DNA isolated from λ 1 .201 -λ 1 .205 with EcoRI indicated that clone 1 .201 contains the original insert of - 350 bp PCR1 fragment, whereas clones 1 .202, 1 .203, 1 .204 and 1 .205 contain inserts of - 1 1 00, - 4000, - 2600 and - 2200 nt, respectively. F. Isolation of DNA encoding a human 1H calcium channel subunit and construction of DNA encoding a full-length α 1H subunit 1. Reference list of partial human α 1 H clones The full-length σ 1 H cDNA sequence is set forth in SEQ ID NO. 49. A list of partial cDNA clones used to characterize the α 1 H sequence and the nucleotide position of each clone relative to the full-length σ 1 H cDNA sequence is shown below. The isolation and characterization of these clones are described below. 1 .305 nt 1 to 3530 of SEQ ID No. 49 1 .205 nt 2432 to 4658 of SEQ ID No. 49 1 .204 nt 31 54 to 5699 of SEQ ID NO. 49 PCR1 nt 4271 to 461 0 of SEQ ID NO. 49 1 .202 nt 4372 to 5476 of SEQ ID No. 49 1 .203 nt 3891 to 7898 of SEQ ID No. 49 2. Characterizetion of the clones DNA sequencing of each insert revealed that clone 1 .202 contains 1 , 105 bp insert corresponding to nt 4372 to 5476 of SEQ ID No. 49; clone 1 .203 contains 4,008 bp insert corresponding to nt 3891 to 7898 of SEQ ID No. 49; clone 1 .204 contains 2,546 bp insert corresponding to nt 31 54 to 5699 of SEQ ID NO. 49; and clone 1 .205 contains 2,227 bp insert corresponding to nt 2432 to 4658 of SEQ ID No. 49. These four DNA clones contain overlapping sequences that encode an open reading frame of approximately 6.6 kb that encodes a majority of the σ 1H subunit, including the entire carboxy terminus and the in-frame translational stop codon. DNA encoding the 5'-end of the human σ 1 H calcium channel subunit was isolated using a 548 bp EcoRI-Ncol restriction endonuclease fragment from the 5'-end of clone 1 .205 (nt 2432 to nt 2979 SEQ ID No. 49) to rescreen the TT cell cDNA library under high stringency conditions. Briefly, DNA encoding the amino terminus of human σ 1 H calcium containing inserts of > 3.5 kb was incubated with the purified restriction fragment and hybridized at 42 °C and washed under high stringency conditions as described above. One recombinant, clone 1 .305, was identified that contains a 3,530 nucleotide insert that shares at its 3' end approximately 1 .1 kb of sequence identity with the 5'-end of clone 1 .205 ( ~ nt 2432 to nt 3530 SEQ ID No. 49) and also contains 2.4 kb of sequence upstream of the EcoRI site located at the 5'-end of clone 1 .205 (nt 2433 to 2438 SEQ ID No. 49) . This sequence encodes the ATG initiation codon (nt 249 to nt 251 SEQ ID No. 1 2) and 1 ,094 amino acids of the amino terminus of the σ 1 H subunit as well as 248 bp of 5'-untranslated sequence, including a consensus ribosome binding site (nt 244 to nt 248 of SEQ ID No. 49) . Two other recombinants were also identified (SEQ ID NOs. 1 3 and 14) that share approximately 1 .1 kb of sequence identity with the 3'-end of clone 1 .305 but differ in the length of the DNA sequence corresponding to the extended intracellular loop located between transmembrane Domains I and II. 3. Construction of a full-length cr 1H 1 -encoding DNA clone Portions of these partial cDNA clones can be ligated to generate a full-length σ 1 H cDNA using common restriction endonuclease sites shared amongst the σ 1 H -encoding fragments. A full-length σ 1 H encoding clone was constructed by 1 ) combining the DNA encoding the 5'-end of σ 1 H present in clone 1 .305 with clone 1 .205 using a common EcoRI site (nt 2433 to 2438 SEQ ID No. 49); and 2) the resulting clone, which encodes the amino terminus of σ 1 H was combined with the carboxyl terminal sequences of σ 1 H encoded in clone 1 .203 using the common EcoRV restriction endonuclease site shared between clone 1 .205 and 1 .203 (nt 451 7-4522 of SEQ ID NO. 1 2) . The resulting full-length human σ 1 H calcium channel subunit is 2,353 amino acid residues in length (SEQ ID NO. 12) . The expression construct was assembled in pCDNAI (Invitrogen, San Diego, CA) and included a consensus ribosome binding site (RBS) followed by the full-length σ 1 H coding sequence (see, for a description of pcDNAI -based vectors containing the RBS, see, e.g. , in International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/1 49,097, U.S. Patent No. 5,851 ,824, and U.S. Patent No. 5,846,756). The resulting construct was designated pcDNA1 σ 1 H RBS. EXAMPLE 2: Cloning of human calcium channel σ 1H 2 subunit T-type channel currents are heterogeneous among different cell types, with varying biophysical and pharmacological profiles, and as shown in this and the following examples can result from expression of different a subunit subtypes in different cells. A. Cloning of σ 1H 2 As described above, PCR Primers-1 and -2, chosen based on an alignment of the human σ 1A -σ 1E sequences in the central cytoplasmic loop ll/lll region and Primer-3 (GA(A/G)ATGATGATGAA(A/G)GT SEQ ID NO. 10) was chosen after considering σ related C. elegans sequences in cosmid C54D2 aligned with the human α^-encoding nucleic acid sequences. The ^-related encoding nucleic acids were amplified in two steps from TT cellular poly(A) + RNA, using Primers-1 and -2 first in a degenerate amplification reaction followed by Primer-3 and Primer-2 in a nested PCR amplification. This resulted in amplification of a 340 nucleotide fragment that encodes a portion of the σ 1 H subunit. This amplification product was used as a probe to screen the library to isolate nucleic acid clones encoding a full-length 1 H subunit. Using a primer base on the σ 1 H 1 sequence and RT-PCR on various tissues, transcripts with an in-frame deletion relative to α l H 1 were identified and isolated from the TT cell library. Fragments spanning this deletion were isolated and, when lined up matched the σ 1 H . 1 f sequence except for a 957 base pair deletion. A full-length clone, designated σ 1 H 2 (see SEQ ID NO. 1 6), was constructed from among these fragments, and inserted in the pcDNAI with the RBS as for ct H . σ 1 H . 2 transcripts were identified in all tissues examined. Nucleic acid encoding σ 1 H . 2 results from an alternately spliced RNA and has a 957 nucleotide in-frame deletion relative to σ 1 H .-, , as detected in the PCR products from numerous tissues and cells, including TT cellular cDNA,, amygdala cDNA, caudate nucleus cDNA, putamen cDNA, heart cDNA, kidney cDNA and liver cDNA. PCR primers were: (i) 5'-primer corresponding to the sense strand of σ1 H-1 at nucleotide 1 373 through 1 393; (ii) 3'-primer corresponding to the antisense strand of σ1 H-1 at nucleotide 2657 through 2680. SEQ ID Nos. 1 2 and 1 5 show the nucleotide sequence of σ 1 H .ι - The coding sequence for σ 1hM begins at nucleotide 249 and ends at 7310. (SEQ ID Nos. 1 2 and 1 5 differ in minor respects, e.g., amino acid 2230 (bases 6983-6985) is Asp (GAC) in the SEQ ID No. 1 5 and Glu (GAA) in SEQ ID No. 1 2) . SEQ ID No. 1 6 shows the nucleotide sequence of the σ 1 H . 2 splice variant. The coding sequence for σ 1 H"2 begins at 249 and ends at 6353. B. Summary Nucleic acid clones encoding full length σ1 H T-type channel subtype were isolated from TT cells. Although similar in overall nucleotide sequence topography to other previously cloned HVA a^ subunits, the σ 1 H subunit contained several unusual features, including a large ll-lll domain loop, absence of the common a interaction domain, and altered ion selectivity properties. Two isoforms of σ 1 H designated σ 1 H . 1 and σ 1 H _ 2 were identified. The first σ 1 H 1 is the larger of the two, and the second α 1 H . 2 is the smaller of the two containing a 957 nucleotide deletion in the ll-lll loop relative to σ 1 H .ι - The nucleotide sequence of a m . is set forth in SEQ ID No. 1 2 and No. 1 5 and that of σ 1 H . 2 is set forth in SEQ ID NO. 1 6. σ 1H . 2 contains a 957 nucleotide deletion relative to σ 1hM which results in a loss of 31 9 amino acids (amino acids 470-788 of σ 1 H -ι) from within the intracellular loop between domains II and III . The splice variant deletion was identified by PCR in all cells and tissues examined. These include TT-cells, amygdala, caudate nucleus, putamen, heart, kidney and liver cells. In the brain expression is primarily in the amygdala, caudate nucleus and putamen. Liver, kidney and heart have high levels. The coding sequence for σ1 H-1 begins at nucleotide 249 and ends at nucleotide 7310 while the coding sequence for σ 1 H . 2 begins at nucleotide 249 and ends at nucleotide 6353. Polyclonal antiserum was raised to the putative ll-lll intracellular loop domain of the σ1 H subunit. Following transient expression in HEK293 cells a protein of the appropriate size was detected by SDS- PAGE and Western blotting. Functional characterization of human σ 1 H channels is provided in EXAMPLE 3. EXAMPLE 3: Biophysical and Pharmacological properties of channels containing σ 1 H 1 and α 1H 2 subunits A. Materials and Methods Materials and methods for biophysical and pharmacology study of calcium channel subunits are described in this EXAMPLE and EXAMPLE 4 below with reference to previously cloned subunits. Such methods or other similar methods known to those of skill in the art have been used to study these properties of human σ 1 H .., subunits as described in this Example. Electrophysiology: HEK293 cells were transiently transfected with 6 μg pcDNA1 σ 1 H RBS using a standard Ca 2 + phosphate procedure (see, e.g. , EXAMPLE below, see, also Williams et aL_ ( 1 992) Neuron. 8:71 -84, for transfection procedure). pCMVCD4, a human CD expression plasmid, was included in the transfections as a marker to permit the identification of transfected cells. Prior to recording, cells were washed with mammalian Ringer's solution, incubated for approximately 10 min in a solution containing a 1 /1000 dilution of M-450 CD4 Dynabeads (Dynal Inc., Lake Success, NY) and rewashed with mammalian Ringer's solution to remove excess beads. Functional expression of 1 H channels in transfected cells was evaluated 24-48 hours following transfection using the whole-cell patch clamp technique. All recordings were performed on single cells at room temperature ( 1 9-24°C) . Whole-cell currents were recorded using an Axopatch-200A (Axon Instruments, Foster City, CA) or anEPC-9 (HEKA elektronik, Lambrecht, Germany) patch clamp amplifier, low-pass filtered at 1 kHz (-3 dB, 8-pole Bessel filter) and digitized at a rate of 10 kHz, unless otherwise stated. Pipettes were manufactured from borosilicate glass (TW1 50, WPI, Sarasota, FI), coated with Sylgard (Dow Corning Midland, Ml), and had a resistance of 1 .1 -2.0 MΩ when filled with internal solution. Series resistance was 2-5 MΩ and 70-90% series resistance compensation was generally used . The pipette solution contained (in mM): 1 35 CsCI, 10 EGTA, 1 MgCI 2 , 10 HEPES (pH 7.3, adjusted with Cs-OH) . The external solution contained (in mM) : 1 5 BaCI 2 or CaCI 2 , 1 50 Choline C1 , 1 MgCI 2 , 5 TEA-OH and 1 0 HEPES (pH 7.3, adjusted with HC1 ) . Single channel recordings were obtained using the cell-attached configuration of the patch-clamp technique. The pipette solution contained (in mM) : 1 1 0 BaCI 2 , 10 HEPES (pH 7.3, adjusted with TEA-OH) . The membrane potential of individual HEK293 cells was set to zero with a solution containing (in mM): 140 K-aspartate, 5 EGTA, and 1 0 HEPES (pH 7.3) . Membrane potentials in the single channel recordings were not corrected for liquid junction potential offset ( + 1 2 mV) . Linear leak and residual capacitive currents were on-line subtracted using a P/4 protocol (whole-cell recording) or scaled single-channel sweeps with no activity (single-channel recordings). Drugs: Mibefradil (Ro 40-5967) was a gift from F. Hoffman- LaRoche. Nimodipine and (-)BayK-8644 were obtained from Research Biochemicals (Natick, MA) . The peptide toxins ω-CgTx GVIA (conotoxin) and cu-CmTx MVIIC (conotoxin) were obtained from Bachem (Torrance, A) . All remaining compounds were obtained from Sigma. Stock solutions were prepared in dimethl sulfoxide (amiloride, nimodipine), ethanol ((- )BayK-8644) or water (verapamil, mibefradil, ethosuximide, ω-CmTx GVIA and tt/-CmTx MVIIC) and stored at 4°C. Drugs were prepared fresh on each experimental day from stock solutions and applied via peristaltic pump at a flow rate of < 0.5 ml/min. The maximal solvent concentration in the final test solution was < 0.1 % . At these concentrations these solvents ha no effect on σ 1 H -mediated currents. Xenopus oόcyte studies: Xenopus laevis frogs were purchased from Nasco (Fort Atkinson, Wisconsin) . Oόcytes were incubated in Ca 2 + - free solution containing 88 mM NaCI, 1 mM KCI, 0.82 mM MgS0 4 , 2.4 mM NaHC0 3 , 1 0 mM Hepes and 1 .5 mg/ml collagenase A (Worthington, Freehold NJ; Type 4, 1 .5 hr and subsequently Sigma, St. Louis, MO, Type 1 A, 0.5 hr.) . Following collagenase treatment, oόcytes were transferred to frog Ringer's solution that contained 88mM nACI, 1 mM KCI, 0.91 mM CaCI 2 , 0.82 mM MgS0 4 , 0.33 mM Ca(N0 3 ) 2 , 2.4 mM NaHC0 3 and 10 mM Hepes. Under these conditions, manual removal of the follicle cell layer was not required . Oόcytes were injected with 50 ng ( 1μg/ml) of in vitro transcripts encoding the σ 1 H subunit and incubated for 3-5 days at 1 9°C prior to recording . The incubation medium was frog Ringer's solution containing penicillin/streptomycin (Sigma; 10 ml/L), gentamicin (Sigma; 1 ml/L and 5% heat-inactivated horse serum (Gibco, Gaithersburg, MD) . Microelectrodes were pulled on a horizontal puller (Model P80, Sutter Instruments, Novato, CA); filled with 3 M KCI; and selected for resistances in the range of 0.5-2.0 MΩ. Data were recorded using a GeneClamp 500; digitized at 1 -5 KHz; and stored on magnetic disks for analysis offline using pClamp or Axograph software (Axon Instruments) . Ba 2 + or Ca 2 + currents were recorded in a solution containing 36 mM TEA-OH, 2.5 mM KOH, 75 mM mannitol, 10 mM HEPES and 1 5 mM Ba(OH) 2 or Ca(OH) 2 , respectively at pH 7.3. Currents were leak-subtracted using the P/6 protocol. To block Ca 2 + -activated chloride currents, niflumic acid (300μM) was included in experiments where the relative permeability of 1 H channels to Ba 2 + or Ca 2 + was measured. All values are reported as mean ± S.D. unless stated otherwise. Drugs (above) were applied via a gravity-fed perfusion system. At the concentrations used herein, solvents had no effect on σ 1 H - mediated currents. B. Electrophysiology 1 . Current-Voltage Properties The rapid inactivation of a H Ca 2 + channels was strongly voltage- dependent. The current decay was best described with an exponential function with time constants ranging from 42.2 ± 7.8 to 8.8 ± 3.8 ms at membrane potentials between -50 and + 30 mV (n = 6; data not show) . Activation kinetics of σ 1 H .-, Ca 2 + channels were also voltage- dependent with time constants ranging from 9.9 ± 4.7 to 0.9 ± 0.3 ms for membrane potentials between -50 and + 30 mV (n = 8; data not shown). 1 H . ! Ca 2 + channels inactivated completely during the 1 50-ms depolarization. Recovery from inactivation occurred within a period of - 3 s with a fast component (r = 37 ± 9 ms; 1 6.5 ± 4.6% of all channels) and a slow component (r = 37 ± 61 ms; 78 ± 8.5% of all channels; n = 3; data not shown) . To confirm the biophysical properties of recombinant σ 1 H channels observed in whole-cell recordings from HEK293 cells, the functional expression of σ 1 H in Xenopus oόcytes was tested. Substantial currents ( < 1 μA) after injection of σ 1 H transcripts alone was observed. The current-voltage relationship for Ba 2 + or Ca 2 + from traces determined. Following transient transfection of HEK293 cells with a DNA encoding the σ 1 H subunit, Ba 2 + currents that were rapidly activating and inactivating were observed. Ba 2 + currents ( 1 5 mM) elicited by step depolarizations to various test potentials from a holding potential of -90- mV were measured. Currents were activated at a test potential of -50 mV, peaked between -20 and -1 0 mV, and reversed at a membrane potential more positive than + 60 mV. Similar results were obtained with Ca 2 + (15 mM) as the charge carrier. 2. Voltage-Dependence of Activation and Inactivation FIGURE 1 shows the voltage-dependence of activation (m∞) and steady-state inactivation (h) of human σ 1H calcium channels expressed transiently in HEK cells. Voltage-dependence of activation (moo) was determined from tail current analysis. Tail currents were normalized with respect to the maximum peak tail current obtained at +60 mV and were plotted (open symbols, mean ± SEM; n = 11) vs. test potential. Data were fitted by the sum of two Boltzman function m∞ = FA*[1 +exp (- (Vtest-V1/2,A)/KA)]1 +F B *[1 +exp(-(V test -V 1/2,B )/k B )] 1 , F A = 0.67, V 1/2A = - 21.5mV, k A = 7.5, F B = 0.33, V 1/2B = 25.5 mV, k B =14.7. Steady-state inactivation (hoo) was determined from a holding potential of -100 mV by a test pulse to -20 mV (p1), followed by a 20 second prepulse from -100 mV to -10 mV in 5 mV decrements (pHold) preceding a second test pulse to -20 mV (p2). Normalized current amplitudes were plotted (closed symbols, mean ± SEM; n = 9) vs. holding potential. Data were fitted by a Boltzman function h∞ = [1 +exp((V hold -V 1/2 )/k)] "1 , V 1/2 = -63.9 mV, k = 3.9mV. 3. Tail Current Deactivation Tail current deactivation profiles for a H calcium channels in transiently transfected HEK cells were studied. One hallmark of LVA channels is their slow rate of deactivation, which is reflected in a show decay of tail currents. The time constant of this decay is —10-fold slower for LVA channels (2-12 ms) than for HVA channels <300μs. A slow decay of a λH . mediated tail currents over a period of -15 ms was observed. In contrast to the monoexponential decay of the tail currents reported for many native T-type Ca 2+ channels, tail currents from σ 1H .., channels showed a biexponential decay. At a test potential of -20 mV, the decay rate of the slow component, comprising 88.1 ± 33.8% of the total current, was 2.1 ± 1 .06 ms (n = 6), which is similar to those observed in native T-type Ca 2 + channels. The decay rate of the faster component was 0.64 ± 0.21 ms (n = 6) . Slow decay of α^^-mediated tail currents were observed over a period of 1 5 ms. The voltage dependence of activation of σ 1 H .-, containing Ca 2 + channels was determined from tail-current analysis. Normalized tail- current amplitudes were plotted as a function of test potential and revealed a biphasic activation curve that was well fitted by the sum of two Boltzmann functions (Figure 1 ) . The potentials for half-maximal activation of the individual Boltzmann terms were as follows: V, Λ A : -25.1 ± 3 3.0 mV; and V, /a B : + 25.5 ± 3 9.9 mV (n = 1 1 ) . A value similar to V, /2 A has been reported previously for voltage dependence of activation of T-type CA 2 + channels in the human TT cell line (-27 mV) . The value of the second Boltzmann term V, Λ B is somewhat similar to that reported for HVA Ca 2 + channels. Using a similar protocol, tail currents of HVA Ca 2 + channels decay with time constants of < 300 μs, whereas with σ 1 H the most prominent at test potentials close to V, /2 B . The availability of σ 1 H containing Ca 2 + channels for opening was dependent on the membrane for potential as shown in FIGURE 1 . The potential for half-maximal steady-state inactivation (V, /2 ) was - 63.2 ± 2.0 mV (n = 9) . 4. Kinetics of Activation and Inactivation of α 1H Channels FIGURE 2 shows the kinetics of activation (FIGURE 2A) and inactivation (FIGURE 2B) of human σ 1 H calcium channels. Kinetics of activation and inactivation were determined from current traces by fitting an exponential function to rising (FIGURE 2A) or declining (FIGURE 2B) phase of the current. The voltage-dependence for activation and inactivation follows approximately an exponential function. 5. Recovery from Inactivation Recovery of σ 1 H channels expressed transiently in HEK293 cells from inactivation induced by using a double pulse protocol using depolarizing pulses to -20mV was evaluated. The fraction of recovered channels was plotted vs. interpulse interval and the data point were fitted by a bi-exponential function in the form l = Ao + A1 exp(-t/r1 ) + A2exp(- t/r2). r1 :35 ms, A1 :0.1 65, r2:337 ms, A2:0.788. 6. Single-Channel Recording from Human σ 1 H calcium channels Single-channel properties of α 1 H Ca 2 + channels in HEK293 cells were determined in cell-attached recordings with 1 10 mM Ba 2 + as the charge carrier. Single-channel recordings at a test potential of -30 mV from a patch that contains at least three σ 1 H showed that channel openings occurred in bursts and were clustered mainly in the first third of the 100- ms depolarizing pulse, especially with stronger depolarizations. Occasionally, channel activity was spread throughout the entire sweep. The time course of the ensemble-averaged current recorded at -30mV in 1 10 mM Ba 2 + was similar to the σ 1H whole-cell Ba 2 + current recorded at - 40 mV in 1 5 mM Ba 2 + . The currents were compared at different potentials to compensate for the shift in the activation curve to more positive potentials due to the increase in divalent concentration. The unitary current-voltage relationship yielded a unitary slope conductance of 9.06 ± 0.22 pS (n = 4) . C. Biophysical Characterization of Human σ 1 H calcium channels in Xenopus Oocytes 1 . Overview Cloned human σ 1 H calcium channels were characterized further by transient expression of σ 1 H l mRNA in Xenopus oόcytes. Injection of a HA mRNA alone resulted in expression of large currents, i.e., typically > 1μA when recording in 1 5 mM Ba 2 + . The σ 1 H channels were activated at approximately -50 mV with peak responses between -30 mV and -40 mV, which is consistent with low voltage activated channels. Permeability of the σ 1H channels to Ca 2+ was slightly greater than to Ba 2 + . In contrast with high voltage channel, the σ 1H channels activated slowly (r=5.7 ± 1.0 ms at the peak of the l-V curve, 3.3 ± 0.5 ms at -20mV) and inactivated rapidly (7-= 13.4 ± 1.9 ms at the peak of l-V curve, 12.2 ± 1.5 ms at -20 mV). The σ 1H channels expressed in oόcytes were sensitive to steady-state inactivation at relatively negative membrane potentials (V1/2 = -64.5± 1.0 mV) and recovered quickly from inactivation (r of recovery = 330 ms). These values are very similar to those obtained from 1H channels expressed in HEK293 cells. The Ba 2+ currents through σ 1H channels in oόcytes were sensitive to blocking by Ni 2+ and Cd 2+ with IC50 values of 6.3μM and 8.3μM, respectively. Of the antagonists tested, only amiloride (IC50=16μM) and mibefradil (IC50 = 2μM) markedly inhibited σ 1H -mediated Ba 2+ currents through σ 1H channels expressed in oόcytes. Taken together the results indicate that σ 1H represents a low- voltage activated calcium channel subunit. 2. Activation and inactivation Properties of 1H Channel Ba 2+ Currents Current-voltage relationships for Ba 2+ (15 mM) currents were recorded from single oocytes injected with mRNA encoding the human σ 1H subunit. Ba 2+ currents were activated at a membrane potential of about -50 mV and peaked at -30 mV. The relative inactivation rates of human σ 1H channels were investigated in different oόcyte preparations and compared with inactivation rates of αr1 A-2αr2bc5?4a channels; σ1B- 1α2b<J?3a channels; and, σ1 E-3αr2b<5?1 b channels. Ba 2+ currents were elicited using a voltage command in the range of -120 mV to -30 mV for σ 1H channels, or -90 mV to 0 mV or + 10 mV for the other respective σ 1A , σ 1B and σ 1E containing channels. The results presented show the relatively electro-negative activation range of σ 1 H channels in comparison with the high-voltage activated σ1 A-2a2b(5-?4a, σ1 B-1 α2b<5/33a and, σ1 E- 3σ2bJ/?1 b calcium channels. 3. Permeability, Inactivation and Biophysical Properties of Human σ 1H Expressed in Xenopus oocytes Permeability and inactivation properties of human σ 1 H channels were investigated in oόcytes by studying Ba 2 + and Ca 2 + currents. The results show that Ba 2 + currents were not significantly larger than Ca 2 + currents in oόcytes expressing the σ 1 H subunit. Results presented in show normalized steady-state inactivation curves for σ 1 H -mediated Ba 2 + currents, where V1 /2 was calculated to be equal to a value of -64.5 ± 1 .0 mV. A double pulse protocol, i.e., with increasing time intervals between pulses, was used to examine the recovery of σ 1 H channels from inactivation. The results of relative recovery of channels plotted against the interpulse interval (ms) and demonstrated that σ 1 H channel currents recovered quickly from inactivation, with an average time constant of 330 ms (n = 5) . 4. Cadmium, Nickel, Amiloride and Mibefradil Antagonize human σ 1H Channel Ba 2+ Currents Cd 2 + was found to antagonize low-threshold human σ 1 H currents in oόcytes in a concentration dependent manner. By plotting the inhibition of Cd 2 + as the percentage of the control Ba 2 + current achieved at different concentration of Cd 2 + , an IC 50 of 1 0.3μM as calculated. Ni 2 + was also found to antagonize low-threshold human σ 1 H channels in oόcyte, and also in a concentration dependent manner. The inhibition of Ba 2 + currents produced by different concentrations of Ni 2 + (n = 4 experiments; n H = 0.84) was tested. The calculated IC 50 for Ni 2 + was 6.3μM. Antagonism by Nl 2 + and Ba 2 + were largely reversible. In addition, each of Amiloride and Mibefradil blocked low-threshold Ba 2 + currents in oόcytes in a concentration-dependent manner giving a calculated IC 50 of 1 61μM for Amiloride; mean of 7 experiments, n H = 0.62) and mean of 2.1 μM for Mibefridil; mean of 4 experiments, n H = 0.71 ) . These results demonstrate that incorporation of an σ 1 H subunit into functional calcium channels in the membranes of cells, conveys the electrophysiologic and biophysical properties of low-voltage activated, particularly T-type, calcium channels upon those channels. The σ 1 H - containing channels were activated rapidly at relatively negative membrane potentials (i.e., V 1 /2 = 64.5 mV), and were also inactivated rapidly (i.e., r= 1 2.2 ms at -20mV) . Peak channel open activity was observed at a membrane potential of -30mV. These channels also exhibited approximately equal permeability for Ca 2 + and Ba 2 + . Pharmacologic properties of σ 1 H containing channels were also consistent with those of other low-threshold calcium channels. They are blocked by Ni 2 + (IC 50 = 6.3μM), Cd 2 + (IC B0 = 10.3μM), Amiloride (IC 50 = 1 6.1μM) and Mibedfradil (IC 50 = 2.1μM). D. Comparison of calcium channels containing human σ 1H subunits expressed in HEK293 Cells with those expressed in Xenopus oocytes TABLE 4 summarizes the biophysical properties of: (i) human α 1H -r containing calcium channels expressed in HEK293 cells, (ii) human σ 1 H . containing channels expressed in Xenopus oocytes, and (iv) native T-type calcium channels expressed in various tissues. TABLE 4 Biophysical properties of 1H -containing Ca 2+ channels b Huguenard (1996) Annual Rev. Physiol.58:329-348; c determined at - 20 mV test potential; n.d. not determined E. Properties of calcium channels containing σ 1H2 subunits Summary Discussion The biophysical properties of σ 1H _ 2 , revealed a shift in the V 1/2 of isochronic inactivation (20 seconds) to -73 mV compared to a V 1/2 of —62.5 mV for a HΛ . The V 1/2 of σ 1H . 2 , thus exhibits a range closer to V 1/2 values reported for certain native T-type calcium channels (Huguenard (1996) Annual Rev. Physiol.58:329-348). For example, under similar recording conditions the V 1/2 of isochronic inactivation for T-channels in rate dorsal horn neurons (DHN) is reported to be -82 mV, while the V 1/2 recorded in rate dorsal lateral geniculate neurons (LGN) is -64 mV. In addition, the V 1/2 of σ 1H . 2 more closely approximates the V1/2 in native rat DHN compared to the value for σ 1H 1 , which, instead, comes closer to the value recorded for T-type calcium channels in LGN. Thus, the observed differences the amino acid sequence of the α 1H 1 and σ 1H . 2 subunits appears linked to differences in tissue distribution of these two different forms of the σ 1H channel. These results also provide basis for understanding the observed different broad ranges of values that have been reported for the V 1/2 inactivation of T-type calcium channels (-100 to -50 mV) in different tissues (see, e.g. , Huguenard (1996) Annual Rev. Physiol.58:329-348). F. Summary of Biophysical Properties of Human σ 1H Containing calcium channels TABLE 5 summarizes the biophysical properties of calcium channels containing the human σ 1H subunits. TABLE 5 Comparison of biophysical parameters of 1H subunits transiently expressed in HEK293 cells using 15 MM Ba 2+ as the charge carrier: σ 1H1 corresponds to the wild type form of the subunit; σ 1H2 to the splice variant form; Steady-state activation from Boltzman fit in the form: moo = Fraction.,* [1 +exp(-(V test -V l/2A )/Slope A )] + (1-Fractιon A )*[1 +exp(-(V test - V 1/2B /Slope B )] ^ Isochronic inactivation (or steady-state inactivation) from Boltzman fit in the form: ∞ = =[1 +exp((V test -V 1/2 )/Slope)] 1 G. Pharmacologic Profile of Human σ 1H calcium channels The sensitivity of σ 1H Ca 2+ channels expressed in HEK293 cells to several agents known to act on VGCCs (Table below) was tested. σ 1H - mediated currents were 16-fold more sensitive to Ni 2+ (IC 50 = 6.6 M) than to Cd 2+ (IC 50 = 104μM). Currents were also inhibited by the T-type channel antagonists amiloride (IC 50 = 1 67μM) and mibefradil (51 .0 ± 10.0% at 1 μM; n = 5) . In contrast, the T-type channel antagonist ethosuximide produced little inhibition of σ 1 H -mediated currents (7.2 ± 1 .8% inhibition at 300 μM; n = 5) . The calcium channel inhibitor verapamil, the L-type antagonist nimodipine, and the L-type agonist (-)- Bay K 8644 had little effect on σ 1 H channels at a concentration of 1 μM. A higher concentration (10 μM) of nimodipine or (-)-Bay K 8644 produced a marked inhibition (43.7 ± 4.1 %, n = 4, and 1 8.1 ± 9.1 %, n = 5, respectively) . The peptide toxins t -CgTx GVIA and ω-CmTx MVIIC at a concentration of 1 μM provided little or no inhibition of σ 1 H -mediated currents. Pharmacological studies reveal the following rank order of potency for inhibition of α^^-containing channels: ni 2 + (IC50: 6.6 μM) = Mibefradil (51 % at 1 μM) > Cd 2 + (IC50: 104 μM) > Amiloride (IC50: 1 67 μM) > > Ethosuximide (7% at 300 μM) . Nimodipine, Verapamil, ω- CgTx GVIA and ω-CmTx MVIIC had little effect (0-1 7%) at a concentration of 1 μM. These findings demonstrate that σ 1H -containing calcium channels have properties corresponding to native LVA, or T-type calcium channels. Table 6 summarizes the pharmacological profile of human σ 1 H containing calcium channels expressed in HEK293 cells. With the exception of ω-CmTx MVIIC, in all cases the charge carrier was 1 5 mM Ba 2 + . In the case of ω-CmTx MVIIC the charge carrier for was 2 mM Ba 2 + because w-CmTx MVIIC was a more effective inhibitor at lower divalent concentrations. Values for % block are mean ± SD(n) . IC 50 values were calculated from sigmoidal curve fitting data (Prism, Graphpad Inc.) for data points from 3 to 6 determinations. TABLE 6 Pharmacology of σ 1 H Ca 2+ Channels Expressed in HEK293 Cells EXAMPLE 4: RECOMBINANT EXPRESSION OF HUMAN NEURONAL CALCIUM CHANNEL SUBUNIT-ENCODING cDNA AND RNA TRANSCRIPTS IN MAMMALIAN CELLS The methods and assays described in this example, may be employed using the nucleic encoding an σ 1 H subunit in place of the σ, subunits exemplified below. Of particular interest are cells that express the σ 1 H subunit alone, as homomers, monomers or multimers, or in combination with selected a 2 subunits. A. Recombinant Expression of the Human Neuronal Calcium Channel a 2 subunit cDNA in DG44 Cells 1 . Stable transfection of DG44 cells DG44 cells (dhfr " Chinese hamster ovary cells; see, e.g. , Urlaub, G. et al. ( 1 986) Som. Cell Molec. Genet. 72:555-566) obtained from Lawrence Chasin at Columbia University were stably transfected by CaPO 4 precipitation methods (Wigler et al. ( 1 979) Proc. Natl. Acad. Sci. USA 76: 1 373-1 376) with pSV2dhfr vector containing the human neuronal calcium channel σ 2 -subunit cDNA for polycistronic expression/selection in transfected cells. Transfectants were grown on 10% DMEM medium without hypoxanthine or thymidine in order to select cells that had incorporated the expression vector. Twelve transfectant cell lines were established as indicated by their ability to survive on this medium. 2. Analysis of a 2 subunit cDNA expression in transfected DG44 cells Total RNA was extracted according to the method of Birnboim ((1 988) Nuc. Acids Res. 76: 1487-1497) from four of the DG44 cell lines that had been stably transfected with pSV2dhfr containing the human neuronal calcium channel a 2 subunit cDNA. RNA ( — 1 5 μg per lane) was separated on a 1 % agarose formaldehyde gel, transferred to nitrocellulose and hybridized to the random-primed human neuronal calcium channel a 2 cDNA (hybridization: 50% formamide, 5 x SSPE, 5 x Denhardt's, 42° C; wash :0.2 x SSPE, 0.1 % SDS, 65 ° C) . Northern blot analysis of total RNA from four of the DG44 cell lines that had been stably transfected with pSV2dhfr containing the human neuronal calcium channel a 2 subunit cDNA revealed that one of the four cell lines contained hybridizing mRNA the size expected for the transcript of the a 2 subunit cDNA (5000 nt based on the size of the cDNA) when grown in the presence of 10 mM sodium butyrate for two days. Butyrate nonspecifically induces transcription and is often used for inducing the SV40 early promoter (Gorman, C. and Howard, B. (1983) Nucleic Acids Res. 7 7: 1 631 ) . This cell line, 44σ 2 -9, also produced mRNA species smaller (several species) and larger (6800 nt) than the size expected for the transcript of the a 2 cDNA (5000 nt) that hybridized to the a 2 cDNA-based probe. The 5000- and 6800-nt transcripts produced by this transfectant should contain the entire a 2 subunit coding sequence and therefore should yield a full-length a 2 subunit protein. A weakly hybridizing 8000-nucleotide transcript was present in untransfected and transfected DG44 cells. Apparently, DG44 cells transcribe a calcium channel a 2 subunit or similar gene at low levels. The level of expression of this endogenous a 2 subunit transcript did not appear to be affected by exposing the cells to butyrate before isolation of RNA for northern analysis. Total protein was extracted from three of the DG44 cell lines that had been stably transfected with pSV2dhfr containing the human neuronal calcium channel a 2 subunit cDNA. Approximately 10 7 cells were sonicated in 300 μl of a solution containing 50 mM HEPES, 1 mM EDTA, 1 mM PMSF. An equal volume of 2x loading dye (Laemmli, U.K. ( 1 970) . Nature 227:680) was added to the samples and the protein was subjected to electrophoresis on an 8% polyacrylamide gel and then electrotransferred to nitrocellulose. The nitrocellulose was incubated with polyclonal guinea pig antisera (1 :200 dilution) directed against the rabbit skeletal muscle calcium channel α 2 subunit (obtained from K. Campbell, University of Iowa) followed by incubation with [ 125 l]-protein A. The blot was exposed to X-ray film at -70° C. Reduced samples of protein from the transfected cells as well as from untransfected DG44 cells contained immunoreactive protein of the size expected for the α 2 subunit of the human neuronal calcium channel ( 1 30-1 50 kDa) . The level of this immunoreactive protein was higher in 44σ 2 -9 cells that had been grown in the presence of 10 mM sodium butyrate than in 44σ 2 -9 cells that were grown in the absence of sodium butyrate. These data correlate well with those obtained in northern analyses of total RNA from 44σ 2 -9 and untransfected DG44 cells. Cell line 44σ 2 -9 also produced a 1 10 kD immunoreactive protein that may be either a product of proteolytic degradation of the full-length α 2 subunit or a product of translation of one of the shorter ( < 5000 nt) mRNA produced in this cell line that hybridized to the α 2 subunit cDNA probe. B. Expression of DNA encoding human neuronal calcium channel a Λ , a 2 and β Λ subunits in HEK cells Human embryonic kidney cells (HEK 293 cells) were transiently and stably transfected with human neuronal DNA encoding calcium channel subunits. Individual transfectants were analyzed electrophysiologically for the presence of voltage-activated barium currents and functional recombinant voltage-dependent calcium channels were analyzed. 1. Transfection of HEK 293 cells Separate expression vectors containing DNA encoding human neuronal calcium channel σ 1 D , a 2 and β Λ subunits, plasmids pVDCCIII(A), pHBCaCHσ 2 A, and pHBCaCH ? 1 a RBS(A), respectively, were constructed as described in International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/1 49,097. These three vectors were used to transiently co-transfect HEK 293 cells. For stable transfection of HEK 293 cells, vector pHBCaCHβ 1b RBS(A) was used in place of pHBCaCHβ l a RBS(A) to introduce the DNA encoding the β. subunit into the cells along with pVDCCIII(A) and pHBCaCHσ 2 A. a. Transient transfection Expression vectors pVDCCIII(A), pHBCaCHσ 2 A and pHBCaCH ? 1 a RBS(A) were used in two sets of transient transfections of HEK 293 cells (ATCC Accession No. CRL1 573) . In one transfection procedure, HEK 293 cells were transiently cotransfected with the σ, subunit cDNA expression plasmid, the a 2 subunit cDNA expression plasmid, the β Λ subunit cDNA expression plasmid and plasmid pCMV gal (Clontech Laboratories, Palo Alto, CA) . Plasmid pCMV Jgal contains the la cZ gene (encoding E. coli yS-galactosidase) fused to the cytomegalovirus (CMV) promoter and was included in this transfection as a marker gene for monitoring the efficiency of transfection. In the other transfection procedure, HEK 293 cells were transiently co-transfected with the α subunit cDNA expression plasmid pVDCCIII(A) and pCMV ?gal. In both transfections, 2-4 x 10 6 HEK 293 cells in a 1 0-cm tissue culture plate were transiently co-transfected with 5 μg of each of the plasmids included in the experiment according to standard CaPO 4 precipitation transfection procedures (Wigler et al. ( 1 979) Proc. Natl. Acad. Sci. USA 76: 1 373-1 376) . The transfectants were analyzed for ?-galactosidase expression by direct staining of the product of a reaction involving β- galactosidase and the X-gal substrate (Jones, J.R. ( 1 986) EMBO 5:31 33- 3142) and by measurement of yS-galactosidase activity (Miller, J.H. (1 972) Experiments in Molecular Genetics, pp. 352-355, Cold Spring Harbor Press) . To evaluate subunit cDNA expression in these transfectants, the cells were analyzed for subunit transcript production (northern analysis), subunit protein production (immunoblot analysis of cell lysates) and functional calcium channel expression (electrophysiological analysis). b. Stable transfection HEK 293 cells were transfected using the calcium phosphate transfection procedure [Current Protocols in Molecular Biology, Vol. 1 , Wiley Inter-Science, Supplement 1 4, Unit 9.1 .1 -9.1 .9 ( 1 990)) . Ten-cm plates, each containing one-to-two million HEK 293 cells, were transfected with 1 ml of DNA/calcium phosphate precipitate containing 5 μg pVDCCIII(A), 5 μg pHBCaCHσ 2 A, 5μg pHBCaCH# 1 b RBS(A), 5 μg pCMVBgal and 1 μg pSV2neo (as a selectable marker). After 10-20 days of growth in media containing 500 μg G41 8, colonies had formed and were isolated using cloning cylinders. 2. Analysis of HEK 293 cells transiently transfected with DNA encoding human neuronal calcium channel subunits a. Analysis of β-galactosidase expression Transient transfectants were assayed for S-galactosidase expression by ?-galactosidase activity assays (Miller, J.H., (1 972) Experiments in Molecular Genetics, pp. 352-355, Cold Spring Harbor Press) of cell lysates (prepared as described in International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/149,097) and staining of fixed cells (Jones, J.R. ( 1 986) EMBO 5:3133-3142). The results of these assays indicated that approximately 30% of the HEK 293 cells had been transfected. b. Northern analysis PolyA + RNA was isolated using the Invitrogen Fast Trak Kit (InVitrogen, San Diego, CA) from HEK 293 cells transiently transfected with DNA encoding each of the a a 2 and ?, subunits and the lacZ gene or the α subunit and the lacZ gene. The RNA was subjected to electrophoresis on an agarose gel and transferred to nitrocellulose. The nitrocellulose was then hybridized with one or more of the following radiolabeled probes: the lacZ gene, human neuronal calcium channel σ 1 D subunit-encoding cDNA, human neuronal calcium channel α 2 subunit- encoding cDNA or human neuronal calcium channel β subunit-encoding cDNA. Two transcripts that hybridized with the σ, subunit-encoding cDNA were detected in HEK 293 cells transfected with the DNA encoding the α α 2 , and β λ subunits and the lacZ gene as well as in HEK 293 cells transfected with the α subunit cDNA and the lacZ gene. One mRNA species was the size expected for the transcript of the α subunit cDNA (8000 nucleotides) . The second RNA species was smaller (4000 nucleotides) than the size expected for this transcript. RNA of the size expected for the transcript of the lacZ gene was detected in cells transfected with the α α 2 and β λ subunit-encoding cDNA and the lacZ gene and in cells transfected with the α subunit cDNA and the lacZ gene by hybridization to the lacZ gene sequence. RNA from cells transfected with the α , α 2 and β subunit-encoding cDNA and the lacZ gene was also hybridized with the α 2 and β subunit cDNA probes. Two mRNA species hybridized to the a 2 subunit cDNA probe. One species was the size expected for the transcript of the a 2 subunit cDNA (4000 nucleotides) . The other species was larger (6000 nucleotides) than the expected size of this transcript. Multiple RNA species in the cells co-transfected with a a 2 and ?, subunit-encoding cDNA and the lacZ gene hybridized to the β subunit cDNA probe. Multiple β subunit transcripts of varying sizes were produced since the β subunit cDNA expression vector contains two potential polyA + addition sites. c. Electrophysiological analysis Individual transiently transfected HEK 293 cells were assayed for the presence of voltage-dependent barium currents using the whole-cell variant of the patch clamp technique (Hamill et al. ( 1 981 ) . Pflugers Arch. 337:85-100) . HEK 293 cells transiently transfected with pCMVβgal only were assayed for barium currents as a negative control in these experiments. The cells were placed in a bathing solution that contained barium ions to serve as the current carrier. Choline chloride, instead of NaCI or KCI, was used as the major salt component of the bath solution to eliminate currents through sodium and potassium channels. The bathing solution contained 1 mM MgCI 2 and was buffered at pH 7.3 with 10 mM HEPES (pH adjusted with sodium or tetraethylammonium hydroxide). Patch pipettes were filled with a solution containing 1 35 mM CsCI, 1 mM MgCI 2 , 10 mM glucose, 10 mM EGTA, 4 mM ATP and 10 mM HEPES (pH adjusted to 7.3 with tetraethylammonium hydroxide) . Cesium and tetraethylammonium ions block most types of potassium channels. Pipettes were coated with Sylgard (Dow-Corning, Midland, Ml) and had resistances of 1 -4 megohm. Currents were measured through a 500 megohm headstage resistor with the Axopatch IC (Axon Instruments, Foster City, CA) amplifier, interfaced with a Labmaster (Scientific Solutions, Solon, OH) data acquisition board in an IBM-compatible PC. PCIa p (Axon Instruments) was used to generate voltage commands and acquire data. Data were analyzed with pClamp or Quattro Professional (Borland International, Scotts Valley, CA) programs. To apply drugs, "puffer" pipettes positioned within several micrometers of the cell under study were used to apply solutions by pressure application. The drugs used for pharmacological characterization were dissolved in a solution identical to the bathing solution. Samples of a 1 0 mM stock solution of Bay K 8644 (RBI, Natick, MA), which was prepared in DMSO, were diluted to a final concentration of 1 μM in 1 5 mM Ba + -containing bath solution before they were applied. Twenty-one negative control HEK 293 cells (transiently transfected with the lacZ gene expression vector pCMV gal only) were analyzed by the whole-cell variant of the patch clamp method for recording currents. Only one cell displayed a discernable inward barium current; this current was not affected by the presence of 1 μM Bay K 8644. In addition, application of Bay K 8644 to four cells that did not display Ba 2 + currents did not result in the appearance of any currents. Two days after transient transfection of HEK 293 cells with α , α 2 and β, subunit-encoding cDNA and the lacZ gene, individual transfectants were assayed for voltage-dependent barium currents. The currents in nine transfectants were recorded. Because the efficiency of transfection of one cell can vary from the efficiency of transfection of another cell, the degree of expression of heterologous proteins in individual transfectants varies and some cells do not incorporate or express the foreign DNA. Inward barium currents were detected in two of these nine transfectants. In these assays, the holding potential of the membrane was -90 mV. The membrane was depolarized in a series of voltage steps to different test potentials and the current in the presence and absence of 1 μM Bay K 8644 was recorded. The inward barium current was significantly enhanced in magnitude by the addition of Bay K 8644. The largest inward barium current ( - 1 60 pA) was recorded when the membrane was depolarized to 0 mV in the presence of 1 μM Bay K 8644. A comparison of the l-V curves, generated by plotting the largest current recorded after each depolarization versus the depolarization voltage, corresponding to recordings conducted in the absence and presence of Bay K 8644 illustrated the enhancement of the voltage-activated current in the presence of Bay K 8644. Pronounced tail currents were detected in the tracings of currents generated in the presence of Bay K 8644 in HEK 293 cells transfected with a , a 2 and β subunit-encoding cDNA and the la cZ gene, indicating that the recombinant calcium channels responsible for the voltage- activated barium currents recorded in this transfected appear to be DHP- sensitive. The second of the two transfected cells that displayed inward barium currents expressed a — 50 pA current when the membrane was depolarized from -90 V. This current was nearly completely blocked by 200 μM cadmium, an established calcium channel blocker. Ten cells that were transiently transfected with the DNA encoding the α. subunit and the lacZ gene were analyzed by whole-cell patch clamp methods two days after transfection. One of these cells displayed a 30 pA inward barium current. This current amplified 2-fold in the presence of 1 μM Bay K 8644. Furthermore, small tail currents were detected in the presence of Bay K 8644. These data indicate that expression of the human neuronal calcium channel σ 1 D subunit-encoding cDNA in HEK 293 yields a functional DHP-sensitive calcium channel. 3. Analysis of HEK 293 ceils stably transfected with DNA encoding human neuronal calcium channel subunits Individual stably transfected HEK 293 cells were assayed electrophysiologically for the presence of voltage-dependent barium currents as described for electrophysiological analysis of transiently transfected HEK 293 cells (International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/1 49,097) . In an effort to maximize calcium channel activity via cyclic- AMP-dependent kinase-mediated phosphorylation (Pelzer, et al. ( 1 990) Rev. Physiol. Biochem. Pharmacol. 7 74: 1 07-207), cAMP (Na salt, 250 μM) was added to the pipet solution and forskolin (10 μM) was added to the bath solution in some of the recordings. Qualitatively similar results were obtained whether these compounds were present or not. Barium currents were recorded from stably transfected cells in the absence and presence of Bay K 8644 ( 1 μM) . When the cell was depolarized to -10 mV from a holding potential of -90 mV in the absence of Bay K 8644, a current of approximately 35pA with a rapidly deactivating tail current was recorded . During application of Bay K 8644, an identical depolarizing protocol elicited a current of approximately 75 pA, accompanied by an augmented and prolonged tail current. The peak magnitude of currents recorded from this same cell as a function of a series of depolarizing voltages were assessed. The responses in the presence of Bay K 8644 not only increased, but the entire current-voltage relation shifted about -10 mV. Thus, three typical hallmarks of Bay K 8644 action, namely increased current magnitude, prolonged tail currents, and negatively shifted activation voltage, were observed, clearly indicating the expression of a DHP-sensitive calcium channel in these stably transfected cells. No such effects of Bay K 8644 were observed in untransfected HEK 293 cells, either with or without cAMP or forskolin. C. Use of pCMV-based vectors and pcDNAI -based vectors for expression of DNA encoding human neuronal calcium channel subunits 1 . Preparation of constructs Additional expression vectors were constructed using pCMV. The full-length 1 D cDNA from pVDCCIII(A) (see International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/1 49,097), the full-length a 2 cDNA, contained on a 3600 bp EcoRI fragment from HBCaCHσ 2 (International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/149,097) and a full-length β, subunit cDNA from pHBCaCHβ l b RBS(A) (see International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/149,097) were separately subcloned into plasmid pCMV/Jgal. Plasmid pCMVβgal was digested with Not\ to remove the lacZ gene. The remaining vector portion of the plasmid, referred to as pCMV, was blunt-ended at the Not\ sites. The full- length σ 2 -encoding DNA and β -encoding DNA, contained on separate EcoRI fragments, were isolated, blunt-ended and separately ligated to the blunt-ended vector fragment of pCMV locating the DNA between the CMV promoter and SV40 polyadenylation sites in pCMV. To ligate the σ 1 D -encoding cDNA with pCMV, the restriction sites in the polylinkers immediately 5' of the CMV promoter and immediately 3' of the SV40 polyadenylation site were removed from pCMV. A polylinker was added at the Not\ site. The polylinker had the following sequence of restriction enzyme recognition sites: I I I I I I I GGCCGC | EcoRI | Saf\ | Pst\ | ΕcoRV | Hind\\\ | XbaW | GT CG | site | site | site | site | site | site | CACCGG I I I I I ' I Not\ Destroys Not The σ 1 D -encoding DNA, isolated as a Bam λ\IXho\ fragment from pVDCCIII(A), was then ligated to X6all/Sa/l-digested pCMV to place it between the CMV promoter and SV40 polyadenylation site. Plasmid pCMV contains the CMV promoter as does pcDNAI , but differs from pcDNA I in the location of splice donor/splice acceptor sites relative to the inserted subunit-encoding DNA. After inserting the subunit-encoding DNA into pCMV, the splice donor/splice acceptor sites are located 3' of the CMV promoter and 5' of the subunit-encoding DNA start codon. After inserting the subunit-encoding DNA into pcDNAI , the splice donor/splice acceptor sites are located 3' of the subunit cDNA stop codon. 2. Transfection of HEK 293 cells HEK 293 cells were transiently co-transfected with the σ 1 D , α 2 and β subunit-encoding DNA in pCMV or with the α w , α 2 and β subunit- encoding DNA in pcDNAI (vectors pVDCCIII(A), pHBCaCHσ 2 A and pHBCaCH/? 1 b RBS(A), respectively (see, International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/149,097) . Plasmid pCMV ?gal was included in each transfection as a measure of transfection efficiency. The results of ?-galactosidase assays of the transfectants (International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/1 49,097), indicated that HEK 293 cells were transfected equally efficiently with pCMV- and pcDNAI -based plasmids. The pcDNAI -based plasmids, however, are presently preferred for expression of calcium channel receptors. D. Expression in Xenopus laevis oόcytes of RNA encoding human neuronal calcium channel subunits Various combinations of the transcripts of DNA encoding the human neuronal σ 1 D , α 2 and β subunits prepared in vitro were injected into Xenopus laevis oόcytes. Those injected with combinations that included a 1 D exhibited voltage-activated barium currents. 1 . Preparation of transcripts Transcripts encoding the human neuronal calcium channel σ 1 D , α 2 and ?, subunits were synthesized according to the instructions of the mCAP mRNA CAPPING KIT (Strategene, La Jolla, CA catalog #200350). As described in International PCT application No. PCT/US94/09230, see, also allowed U.S. application Serial No. 08/149,097, plasmids pVDCC III.RBS(A), containing pcDNAI and the σ 1 D cDNA that begins with a ribosome binding site and the eighth ATG codon of the coding sequence plasmid pHBCaCHffiA containing pcDNAI and an σ 2 subunit cDNA, and plasmid pHBCaCH/? 1 b RBS(A) containing pcDNAI and the β. DNA lacking intron sequence and containing a ribosome binding site were linearized by restriction digestion. The 1 D cDNA- and α 2 subunit-encoding plasmids were digested with Xho\, and the β subunit- encoding plasmid was digested with EcoRV. The DNA insert was transcribed with T7 RNA polymerase. 2. Injection of oocytes Xenopus laevis oόcytes were isolated and defolliculated by collagenase treatment and maintained in 100 mM NaCI, 2 mM KC1 , 1 .8 mM CaC1 2 , 1 mM MgCI 2 , 5 mM HEPES, pH 7.6, 20 μg/ml ampicillin and 25 μg/ml streptomycin at 1 9-25 °C for 2 to 5 days after injection and prior to recording. For each transcript that was injected into the oόcyte, 6 ng of the specific mRNA was injected per cell in a total volume of 50 nl. 3. Intracellular voltage recordings Injected oόcytes were examined for voltage-dependent barium currents using two-electrode voltage clamp methods (Dascal, N. (1 987) CRC Crit. Rev. Biochem. 22:31 7) . The pClamp (Axon Instruments) software package was used in conjunction with a Labmaster 1 25 kHz data acquisition interface to generate voltage commands and to acquire and analyze data. Quattro Professional was also used in this analysis. Current signals were digitized at 1 -5 kHz, and filtered appropriately. The bath solution contained of the following : 40 mM BaCI 2 , 36 mM tetraethylammonium chloride (TEA-CI), 2 mM KCI, 5 mM 4-aminopyridine, 0.1 5 mM niflumic acid, 5 mM HEPES, pH 7.6. a. Electrophysiological analysis of oόcytes injected with transcripts encoding the human neuronal calcium channel a Λ , a 2 and ^-subunits Uninjected oόcytes were examined by two-electrode voltage clamp methods and a very small (25 nA) endogenous inward Ba 2 + current was detected in only one of seven analyzed cells. Oόcytes coinjected with σ 1D , a 2 and β subunit transcripts expressed sustained inward barium currents upon depolarization of the membrane from a holding potential of -90 mV or -50 mV (154 ± 1 29 nA, n = 21 ) . These currents typically showed little inactivation when test pulses ranging from 140 to 700 msec, were administered. Depolarization to a series of voltages revealed currents that first appeared at approximately -30 mV and peaked at approximately 0 mV. Application of the DHP Bay K 8644 increased the magnitude of the currents, prolonged the tail currents present upon repolarization of the cell and induced a hyperpolarizing shift in current activation. Bay K 8644 was prepared fresh from a stock solution in DMSO and introduced as a 10x concentrate directly into the 60 μl bath while the perfusion pump was turned off. The DMSO concentration of the final diluted drug solutions in contact with the cell never exceeded 0.1 % . Control experiments showed that 0.1 % DMSO had no effect on membrane currents. Application of the DHP antagonist nifedipine (stock solution prepared in DMSO and applied to the cell as described for application of Bay K 8644) blocked a substantial fraction (91 ± 6%, n = 7) of the inward barium current in oόcytes coinjected with transcripts of the σ 1 D , a 2 and β subunits. A residual inactivating component of the inward barium current typically remained after nifedipine application. The inward barium current was blocked completely by 50 μM Cd 2 + , but only approximately 1 5% by 100 μM Ni 2 + . The effect of cc-CgTX-GVIA on the inward barium currents in oόcytes co-injected with transcripts of the σ 1 D , a 2 , and β subunits was investigated. cc-CgTX-GVIA (Bachem, Inc., Torrance CA) was prepared in the 1 5 mM BaCI 2 bath solution plus 0.1 % cytochrome C (Sigma) to serve as a carrier protein. Control experiments showed that cytochrome C had no effect on currents. A series of voltage pulses from a -90 mV holding potential to 0 mV were recorded at 20 msec, intervals. To reduce the inhibition of ωCgTX binding by divalent cations, recordings were made in 1 5 mM BaCI 2 , 73.5 mM tetraethylammonium chloride, and the remaining ingredients identical to the 40 mM Ba 2 + recording solution. Bay K 8644 was applied to the cell prior to addition to ωCgTX in order to determine the effect of c CgTX on the DHP-sensitive current component that was distinguished by the prolonged tail currents. The inward barium current was blocked weakly (54 ± 29%, n = 7) and reversibly by relatively high concentrations ( 1 0-1 5 μM) of ωCgTX. The test currents and the accompanying tail currents were blocked progressively within two to three minutes after application of ωCgTX, but both recovered partially as the ωCgTX was flushed from the bath. b. Analysis of oocytes injected with transcripts encoding the human neuronal calcium channel σ 1D or transcripts encoding an σ 1D and other subunits The contribution of the a 2 and β subunits to the inward barium current in oόcytes injected with transcripts encoding the σ 1 D , a 2 and β^ subunits was assessed by expression of the σ 1 D subunit alone or in combination with either the β subunit or the a 2 subunit. In oόcytes injected with only the transcript of a σ 1 D cDNA, no Ba 2 + currents were detected (n = 3) . In oόcytes injected with transcripts of σ 1 D and β encoding DNA, small ( 1 08 ± 39 nA) Ba 2 + currents were detected upon depolarization of the membrane from a holding potential of -90 mV that resembled the currents observed in cells injected with transcripts of σ 1 D , a 2 and β encoding DNA, although the magnitude of the current was less. In two of the four oόcytes injected with transcripts of the α 1 D -encoding and /^-encoding DNA, the Ba 2 + currents exhibited a sensitivity to Bay K 8644 that was similar to the Bay K 8644 sensitivity of Ba 2 + currents expressed in oόcytes injected with transcripts encoding the σ 1 D a -, a 2 _ and β subunits. Three of five oόcytes injected with transcripts encoding the a w and a 2 subunits exhibited very small Ba 2 + currents ( 1 5-30 nA) upon depolarization of the membrane from a holding potential of -90 mV. These barium currents showed little or no response to Bay K 8644. c. Analysis of oόcytes injected with transcripts encoding the human neuronal calcium channel a 2 and/or β Λ subunit To evaluate the contribution of the σ 1 D αysubunit to the inward barium currents detected in oόcytes co-injected with transcripts encoding the a w , a 2 and β subunits, oόcytes injected with transcripts encoding the human neuronal calcium channel a 2 and/or β subunits were assayed for barium currents. Oόcytes injected with transcripts encoding the σ 2 subunit displayed no detectable inward barium currents (n = 5). Oόcytes injected with transcripts encoding a β λ subunit displayed measurable (54 ± 23 nA, n = 5) inward barium currents upon depolarization and oόcytes injected with transcripts encoding the a 2 and β subunits displayed inward barium currents that were approximately 50% larger (80 ± 61 nA, n = 1 8) than those detected in oόcytes injected with transcripts of the β - encoding DNA only. The inward barium currents in oόcytes injected with transcripts encoding the β^ subunit or a 2 and β subunits typically were first observed when the membrane was depolarized to -30 mV from a holding potential of -90 mV and peaked when the membrane was depolarized to 10 to 20 mV. Macroscopically, the currents in oόcytes injected with transcripts encoding the a 2 and /?, subunits or with transcripts encoding the β subunit were indistinguishable. In contrast to the currents in oόcytes co- injected with transcripts of σ 1 D , a 2 and β λ subunit encoding DNA, these currents showed a significant inactivation during the test pulse and a strong sensitivity to the holding potential. The inward barium currents in oόcytes co-injected with transcripts encoding the a 2 and β subunits usually inactivated to 10-60% of the peak magnitude during a 140-msec pulse and were significantly more sensitive to holding potential than those in oόcytes co-injected with transcripts encoding the σ 1 D , a 2 and ?, subunits. Changing the holding potential of the membranes of oόcytes co-injected with transcripts encoding the a 2 and β subunits from -90 to - 50 mV resulted in an approximately 81 % (n = 1 1 ) reduction in the magnitude of the inward barium current of these cells. In contrast, the inward barium current measured in oόcytes co-injected with transcripts encoding the σ 1 D , a 2 and β subunits were reduced approximately 24% (n = 1 1 ) when the holding potential was changed from -90 to -50 mV. The inward barium currents detected in oόcytes injected with transcripts encoding the a 2 and β subunits were pharmacologically distinct from those observed in oόcytes co.-injected with transcripts encoding the σ 1 D , a 2 and ?, subunits. Oόcytes injected with transcripts encoding the a 2 and ?, subunits displayed inward barium currents that were insensitive to Bay K 8644 (n = 1 1 ) . Nifedipine sensitivity was difficult to measure because of the holding potential sensitivity of nifedipine and the current observed in oόcytes injected with transcripts encoding the a 2 and β subunits. Nevertheless, two oόcytes that were co-injected with transcripts encoding the a 2 and β subunits displayed measurable (25 to 45 nA) inward barium currents that were insensitive to nifedipine (5 to 1 0 μM), when depolarized from a holding potential of -50 mV. The inward barium currents in oόcytes injected with transcripts encoding the a 2 and β λ subunits showed the same sensitivity to heavy metals as the currents detected in oόcytes injected with transcripts encoding the σ 1 D , 2 and β Λ subunits. The inward barium current detected in oόcytes injected with transcripts encoding the human neuronal a 2 and /?, subunits has pharmacological and biophysical properties that resemble calcium currents in uninjected Xenopus oόcytes. Because the amino acids of this human neuronal calcium channel β Λ subunit lack hydrophobic segments capable of forming transmembrane domains. It is unlikely that recombinant / -?, subunits alone form an ion channel, but rather that an endogenous σ, subunit exists in oόcytes and that the activity mediated by such an a subunit is enhanced by expression of a human neuronal β subunit. While the subject matter of the invention has been described with some specificity, modifications apparent to those with ordinary skill in the art may be made without departing from the scope of the invention. Since such modifications will be apparent to those of skill in the art, it is intended that this invention be limited only by the scope of the appended claims.

Claims: WHAT IS CLAIMED IS: 1 . An isolated nucleic acid fragment that encodes a low-voltage activated subunit of an animal calcium channel. 2. The nucleic acid of claim 1 , wherein the subunit is an σ 1 H - subunit. 3. The nucleic acid of claim 2, wherein the calcium channel is a mammalian calcium channel. 4. The isolated nucleic acid fragment of claim 2, comprising a sequence of nucleotides that encodes the subunit, wherein the sequence of nucleotides encoding the subunit is selected from among: (a) a sequence of nucleotides that encodes a calcium channel subunit and comprises the coding portion of the sequence of nucleotides set forth in any of SEQ ID Nos. 1 2-1 6; (b) a sequence of nucleotides that encodes an σ 1 H -subunit and hybridizes under conditions of high stringency to DNA that is complementary to an mRNA transcript present in a mammalian cell that encodes an σ 1 H -subunit; (c) a sequence of nucleotides that encodes the subunit that comprises a sequence of amino acids encoded by any of SEQ ID Nos. 1 2-1 6; and (d) a sequence of nucleotides that is degenerate with any of (a), (b) or (c) . 5. The molecule of claim 2, wherein the subunit is an σ 1 H .., subunit or an σ 1 H . 2 subunit. 6. A eukaryotic cell, comprising heterologous nucleic acid that encodes an oysubunit, wherein the σ subunit is encoded by the nucleic acid of any of claims 1 -5. 7 The cell of claim 6, further comprising heterologous nucleic acid that encodes a σ 2 <5-subunit of a calcium channel. 8. The eukaryotic cell of claim 6 or claim 7 that has a functional heterologous calcium channel that contains at least one subunit encoded by the heterologous nucleic acid. 9. The eukaryotic cell of any of claims 6-8 selected from the group consisting of HEK 293 ceils, Chinese hamster ovary cells, African green monkey cells, and mouse L cells. 1 0. A eukaryotic cell with a functional, heterologous calcium channel, produced by a process comprising: introducing into the cell heterologous nucleic acid that encodes at least one subunit of a calcium channel, wherein the subunit is encoded by the nucleic acid of any of claims 1 -5. 1 1 . The eukaryotic cell of claim 10 that is an amphibian oόcyte. 1 2. The eukaryotic cell of claim 8 or claim 1 0, wherein the heterologous calcium channel comprises a plurality of σ 1 H -subunits. 1 3 The eukaryotic cell of claim 1 2, wherein the σ 1 H -subunits comprise a homomer. 14. The eukaryotic cell of any of claims 1 0-1 3, further comprising an σ 2 ό-subunit of a calcium channel. 1 5. The eukaryotic cell of claim 1 0, wherein the heterologous nucleic acid encodes a T-type calcium channel. 1 6. The eukaryotic cell of claim 8 with a functional, heterologous calcium channel, produced by a process comprising: introducing into the cell RNA that encodes an σ 1 H subunit of a calcium channel and optionally introducing into the cell nucleic acid that encodes a β, a 2 δ and/or y-subunit of a calcium channel, wherein: the heterologous calcium channel contains at least one subunit encoded by the heterologous nucleic acid; and the only heterologous ion channels are calcium channels. 1 7. The eukaryotic cell of claim 8 with a functional, heterologous calcium channel, produced by a process comprising: introducing into the cell DNA that encodes an σ 1 H subunit of a calcium channel and optionally introducing into the cell nucleic acid that encodes a β, a 2 δ and/or y-subunit of a calcium channel, wherein: the heterologous calcium channel contains at least one subunit encoded by the heterologous nucleic acid. 1 8. The eukaryotic cell of claim 1 7 selected from the group consisting of HEK 293 cells, Chinese hamster ovary cells, African green monkey cells, mouse L cells and amphibian oόcytes. 1 9. The eukaryotic cell of claim 1 6 selected from the group consisting of amphibian oόcytes. 20. The eukaryotic cell of any of claims 6-1 9, wherein the σ 1 H - subunit is an a H . subunit or an σ 1 H 2 subunit. 21 . The eukaryotic cell of claim 20, wherein the σ 1 H subunit is a human calcium channel subunit. 22. A method for identifying a compound that modulates the activity of a calcium channel that contains an σ 1 H subunit, comprising; suspending the eukaryotic cell of any of claims 8-21 in a solution containing the compound and a calcium channel selective ion: depolarizing the cell membrane of the cell; and detecting the current or ions flowing into the cell, wherein: the heterologous calcium channel includes at least one calcium channel subunit encoded by DNA or RNA that is heterologous to the cell, the current that is detected is different from that produced by depolarizing the same or a substantially identical cell in the presence of the same calcium channel selective ion but in the absence of the compound. 23. The method of claim 22, wherein prior to the depolarization step the cell is maintained at a holding potential which substantially inactivates calcium channels that are endogenous to the cell. 24. The method of claim 23, wherein: the cell is an amphibian oόcyte; the heterologous subunits are encoded by nucleic acid injected into the oόcyte; and the heterologous subunits include an σ 1 H -subunit. 25. The method of claim 24, wherein the subunits encoded by the nucleic acid further comprise a α 2 ό-subunit. 26. The method of any of claims 22-25, wherein the cell is an HEK cell and the heterologous subunit is encoded by heterologous nucleic acid. 27. The method of any of claims 22-26, wherein the α 1 H -subunit is an αm^-subunit or an σ 1 H . 2 -subunit. 28. The method of claim 22, wherein: the heterologous calcium channel includes at least one calcium channel subunit encoded by DNA or RNA that is heterologous to the cell; at least one subunit is an σ 1 H -subunit; the current that is detected is different from that produced by depolarizing the same or a substantially identical cell in the presence of the same calcium channel selective ion but in the absence of the compound. 29. A substantially pure σ subunit encoded by the nucleic acid molecule of any of claims 1 -5. 30. An RNA or DNA probe of at least 1 6 bases in length, comprising at least 1 6 substantially contiguous nucleic acid bases from the sequence of nucleotides of claim 1 that encodes an σ 1 H -subunit of a calcium channel. 31 . The probe of claim 28 that contains at least 30 nucleic acid bases that encode the subunit of a calcium channel. 32. A method for identifying nucleic acids that encode a σ 1 H subunit of a calcium channel subunit, comprising hybridizing under conditions of at least low stringency a probe of claim 28 to a library of nucleic acid fragments;, and selecting hybridizing fragments. 33. The method of claim 30, wherein hybridization is effected under conditions of high stringency. 34. A method for identifying cells or tissues that express a calcium channel subunit-encoding nucleic acid, comprising hybridizing under conditions of at least low stringency a probe of claim 30 or claim 31 with mRNA expressed in the cells or tissues or cDNA produced from the mRNA, and thereby identifying cells or tissue that express mRNA that encodes the subunit. 35. The method of claim 32, wherein hybridization is effected under conditions of high stringency. 36. A method for producing a subunit of a calcium channel, comprising introducing the nucleic acid molecule of any of claims 1 -5 into a host cell, under conditions whereby the encoded subunit is expressed. 37. The method of claim 35, wherein the cell is a eukaryotic cell. 38. A eukaryotic cell, comprising a heterologous calcium channel encoded by nucleic acid encoding an σ-subunit of a calcium channel, wherein the heterologous calcium channel is a low voltage activated channel or a T-type channel. 39. The eukaryotic cell of any of claims 6-21 and 38, wherein the σ-subunit comprises the sequence of amino acids set forth in any of SEQ ID Nos. 1 2-1 6. 40. An isolated nucleic acid molecule, comprising the sequence of amino acids encoded by nucleotides 1 506 to 2627 of SEQ ID No. 12. 41 . The isolated nucleic acid molecule of claim 40, comprising the sequence of nucleotides set forth in nucleotides 1 506 to 2627 of SEQ ID No. 1 2. 42. The nucleic acid of any of claims 1 -5, 40 and 41 that is RNA. 43. The nucleic acid of any of claims 1 -5, 40 and 41 that is DNA. 44. The cell of claim 8, further comprising nucleic acid that encodes a reporter gene construct containing a reporter gene in operative linkage with one or more transcriptional control elements that is regulated by a calcium channel. 45. A method for identifying compounds that modulate the activity of a low-voltage activated calcium channel, the method comprising: comparing the difference in the amount of transcription of a the reporter gene in the cell of claim 44 in the presence of the compound with the amount of transcription in the absence of the compound, or with the amount of transcription in the absence of the heterologous calcium channel, whereby compounds that modulate the activity of the heterologous calcium channel in the cell are identified. 46. The nucleic acid molecule of any of claims 1 -5, 40 and 41 , wherein the calcium channel is a human calcium channel. 47. A screening assay for identifying a compound that modulates the activity of a low-voltage activated (LVA) calcium channel comprising the steps of: contacting the test compound with a cell that expresses a LVA calcium channel; and measuring the activity of the LVA channel in the cell before and after the addition of the test compound or in comparable cell that does not express the LVA channel; and determining that the test compound modulates the activity of the low-voltage calcium channel if the measurement after compound addition is different from the measurement before the compound addition or if the measurement in presence of the receptor is different from the measurement in the absence of the receptor. 48. The method of claim 47, wherein the LVA channel is produced by introducing the a nucleic acid that encodes the LVA into the cell under conditions whereby the encoded LVA is expressed. 49. The method of claim 47 or claim 48, wherein the LVA is a T- type channel. 50. The method of any of claims 47-49, wherein the LVA comprises an σ 1 H -subunit of a calcium channel. 51 . The method of any of claims 47-50, wherein the cell expresses a low-voltage calcium channel having a relative conductance of Ba 2 + of about 5 pS to about 9 pS, an activation time of about 2 to about 8 milliseconds, a kinetics of activation V 1 /2 value of about -60 millivolts to about 26 millivolts, an inactivation time of about 1 0 to about 30 milliseconds, a kinetics of inactivation V 1 /2 value of about -1 00 millivolts to about- 500 millivolts, and a tail deactivation time of about 2 to about 1 2 milliseconds. 52. The screening method of any of claims 47-51 , wherein the isolated nucleic acid molecule comprises a sequence of nucleotides encoding an σ 1 H -subunit of a calcium channel. 53. A compound identified by the method of any of claims 45 and 47-52. 54. A method of identifying compounds for treatment of LVA- type calcium channel mediated disorders, comprising identifying compounds that modulate the activity of LVA-type channels in cells that express channels containing a subunit encoded by the nucleic acid of any of claims 1 -5, 40 and 41 . 55. Compounds identified by the method of 54. 56. The method of claim 54, wherein the channels are produced by introduction of the nucleic acid of any of claims 1 -5, 40 and 41 into cells under conditions whereby channels that contain the encoded subunit are expressed. 57. The method of claim 54 or claim 56, wherein the disorder is selected from among, neurological, endocrinological, cardiovascular, urological, hepatic, respiratory, and vascular disorders.

Inventors: HANS MICHAEL, HARPOLD MICHAEL, STAUDERMAN KENNETH, URRUTIA ARTURO, WASHBURN MARK S, WILLIAMS MARK, HANS, MICHAEL, HARPOLD, MICHAEL, STAUDERMAN, KENNETH, URRUTIA, ARTURO, WASHBURN, MARK, S., WILLIAMS, MARK
Applicants: HANS MICHAEL, HARPOLD MICHAEL, SIBIA NEUROSCIENCES INC, STAUDERMAN KENNETH, URRUTIA ARTURO, WASHBURN MARK S, WILLIAMS MARK, HANS, MICHAEL, HARPOLD, MICHAEL, SIBIA NEUROSCIENCES, INC., STAUDERMAN, KENNETH, URRUTIA, ARTURO, WASHBURN, MARK, S., WILLIAMS, MARK
Application year: 1998
Main classification: C07K14
IPC codes: A61K38, A61K45, A61P1, A61P5, A61P9, A61P11, A61P13, A61P25, C07K14, C12N1, C12N5, C12N15, C12Q1, G01N33