METHODS AND COMPOSITIONS FOR CONTROLLING CELL DEATH AND DISEASE RESISTANCE IN PLANTS

Abstract: The present invention is drawn to methods and compositions for suppressing cell death in plants. Specifically, novel proteins and genes are provided for use in plant transformation. The proteins and genes are useful for activating disease resistance, enhancing plant cell transformation efficiency, engineering herbicide resistance, genetically targeting cell ablations, and other methods involving the regulation of cell death in plants.

Description: Methods and Compositions for Controlling Cell Death and Disease Resistance in Plants Field of the Invention The invention relates to the genetic manipulation of plants, particularly to novel genes and proteins and their uses in regulating cell death and disease resistance in plants. Background of the Invention A host of cellular processes enable plants to defend themselves from disease caused by pathogenic agents . These processes apparently form an integrated set of resistance mechanisms that is activated by initial infection and then limits further spread of the invading pathogenic microorganis . Subsequent to recognition of a potentially pathogenic microbe, plants can activate an array of biochemical responses. Generally, the plant responds by inducing several local responses in the cells immediately surrounding the infection site. The most common resistance response observed in both nonhost and race- specific interactions is termed the "hypersensitive response" (HR) . In the hypersensitive response, cells contacted by the pathogen, and often neighboring cells, rapidly collapse and dry in a necrotic fleck. Other responses include the deposition of callose, the physical thickening of cell walls by lignification, and the synthesis of various antibiotic small molecules and proteins. Genetic factors in both the host and the pathogen determine the specificity of these local responses which can be very effective in limiting the spread of infection. Many environmental and genetic factors cause general leaf necrosis in maize and other plants. In addition, numerous recessive and dominant genes have been reported which cause discreet necrotic lesions to form. These lesion mutants mimic disease lesions caused by various pathogenic organisms of maize. For example, Lesl , a temperature- sensitive conditional lethal mutant, mimics the appearance of Helminthosporium maydis on susceptible maize. Many genes causing necrotic lesions have been reported. The pattern of lesion spread on leaves is a function of two factors : lesion initiation and individual lesion enlargement . The lethal leaf spot -1 (llsl ) mutation of maize is inherited in a recessive monogenic fashion and is characterized by the formation of scattered, necrotic leaf spots (lesions) that expand continuously to engulf the entire tissue. Since llsl spots show striking resemblance to lesions incited by race 1 of Cochliobolus (Helminthosporium) carbonum on susceptible maize, this mutation has been grouped among the class of genetic defects in maize called "disease lesion mimics." Lesion mimic mutations of maize have been shown to be specified by more than forty independent loci. These lesion mimic plants produce discreet disease-like symptoms in the absence of any invading pathogens. It is intriguing that more than two thirds of these mutations display a partially dominant, gain-of-function inheritance, making it the largest class of dominant mutants in maize, and suggesting the involvement of a signalling pathway in the induction of lesions in these mutations. Similar mutations have also been discovered in other plants including arabidopsis and barley. Despite the availability of the large number of lesion mimic mutations in plants, the mechanistic basis and significance of this phenomenon, and the wild-type function of the genes involved, has remained elusive. The understanding of the molecular and cellular events that are responsible for plant disease resistance remains rudimentary. This is especially true of the events controlling the earliest steps of active plant defense, recognition of a potential pathogen and transfer of the cognitive signal throughout the cell and surrounding tissue. Diseases are particularly destructive processes resulting from specific causes and characterized by specific symptoms. Generally the symptoms can be related to a specific cause, usually a pathogenic organism. In plants, a variety of pathogenic organisms cause a wide variety of disease symptoms. Because of the lack of understanding of the plant defense system, methods are needed to protect plants against pathogen attack. Summary of the Invention Compositions and methods for suppressing cell death and controlling disease resistance in plants are provided. The compositions, cell death suppressing proteins and the genes encoding such proteins, are useful for activating disease resistance, enhancing plant cell transformation efficiency, engineering herbicide resistance, genetically targeting cell ablations, and other methods involving the regulation of cell death and disease resistance in plants. Additionally, novel promoter sequences are provided for the expression of genes in plants. Brief Description of the Drawings Figure 1 sets forth the organization of the 3kb EcoRI restriction fragment containing 11s sequence. Figure 2 shows that a single transcript was detected when mRNA from mature leaves was probed with the llsl transcript . Figure 3 shows the preferred sites for possible modification of the protein to alter protein activity (SEQ ID NOS 2 & 5-61, respectively) . Detailed Description of the Invention The invention is drawn to compositions and methods for controlling cell death and disease resistance in plant cells. The compositions are proteins, ring-hydroxyl ting dioxygenases, which act to control cell death and regulate disease resistance in plants. The proteins and genes encoding them can be used to regulate cell death and disease resistance in transformed plant cells as well as a variety of other uses. The proteins are useful in resistance to pathogens and survival of the cells particularly after pathogen attack. One aspect of the invention is drawn to proteins which are involved in the degradation of plant phenolics, cell death-suppressing and disease resistance proteins. Such proteins are characterized by containing two consensus motifs, a Rieske-type iron-sulfur binding site, and a mononuclear iron-binding site, and function as aromatic ring-hydroxylating (ARH) dioxygenases. The Rieske motif contains two cysteine and histidine residues responsible for binding an iron atom cofactor. Plant proteins containing at least one of the motifs have been identified and can be used in the methods of the present invention.. Alternatively, proteins from bacteria with the Rieske motif are known in the art and can be used in the methods of the invention. Bacterial proteins of particular interest are ring- hydroxylating dioxygenases, particularly those from the cyanobacterium Synechocystis . See, for example, Gibson et al . (1984) Microbial degradation of organic compounds , 181- 252. D.T. Gibson, ed. (New York: Marcel Dekker), pp. 181- 252. The cell death-suppressing and disease resistance proteins of the invention encompass a novel class of plant proteins. The amino acid sequence of the llsl protein isolated from maize is set forth in SEQ ID NOS 1 & 2 , respectively. However, the proteins are conserved in plants. Thus, as discussed below, methods are available for the identification and isolation of genes and proteins from any plant. Likewise, sequence similarities can be used to identify and isolate other bacterial genes and proteins. The proteins function to inhibit the spread of cell death and control disease resistance in plants. Therefore, the proteins are useful in a variety of settings involving the regulation of cell death and control of disease resistance in plants. The Rieske motif exhibited by the proteins of the invention is shared by a class of enzymes known as ring- hydroxylating dioxygenases. The motif contains two cysteine and histidine residues responsible for binding an iron atom cofactor - residues that are shared by other proteins termed Rieske iron-sulfur proteins. The bacterial genes included in the proteins of the invention are known as catabolic operons . Thus, it is predicted that the plant proteins are related to the degradation of phenolic compound (s) . In fact, a para-coumaric ester accumulates in llsl lesioned plants, but not in normal-type siblings or wild-type siblings inoculated with the fungus Cochliobolus heggerostrophus. While the present invention is not dependent upon any particular mechanism of action, it is believed that the cell death-suppressing function of the novel protein may be mediated by the detoxification of a phenolic compound whose cell damaging effects are fueled by light harvested by photosynthetically- functional pigments in the leaf . Modifications of such proteins are also encompassed by the present invention. Such modifications include substitution of amino acid residues, deletions, additions, and the like. For example, the protein can be mutagenized in such a way that its activity is reduced, but not completely abolished. See, for example, Jiang et al . (1996), J. Bacterial , 178:3133-3139, where the Tyr-221 from the mononucleate iron binding site of toluene dioxygenase was changed to Ala. This change resulted in a reduction in activity to 42% of the normal activity. A change of Tyr-266 to Ala reduced the activity to 12%. In the same manner, amino acid changes, particularly changes from Tyr to Ala, of the sequence of the proteins of the present invention can lead to increases or decreases in activity. Figure 3 sets forth potential modifications which may alter expression of the resulting protein. See also SEQ ID NOS 2 & 5-61, respectively. Such modifications can result in dominant negative inhibitors of the wild type protein. Using these sequences, the expression of llsl can be regulated such that disease resistance can be obtained in the absence of lesions . After each modification of the protein, the resulting protein will be tested for activity. To test for activity, plants can be transformed with the DNA sequence and tested for their response to a fungal pathogen. Of particular interest are changes that result in a reduction of activity. Such changes will confer disease resistance, yet not result in the lesion phenotype. These modified proteins, and the corresponding genes, will be useful in disease defense mechanisms in plants. Accordingly, the proteins of the invention include naturally occurring plant and bacterial proteins and modifications thereof. Such proteins find use in preventing cell death and controlling disease resistance. The proteins are also useful in protecting plants against pathogens. In this manner, the plant is transformed with a nucleotide sequence encoding the protein. The expression of the protein in the plant prevents cell death and confers resistance to infection by plant pathogens. The nucleotide sequences encoding the novel proteins are also provided. The llsl gene from maize encodes the novel maize protein which inhibits the spread of cell death from wounding or internal stresses that occur during photosynthesis. The maize gene can be utilized to isolate homologous genes from other plants, including Arabidopsis, sorghum, Brassica, wheat, tobacco, cotton, tomato, barley, sunflower, cucumber, alfalfa, soybeans, sorghum, etc. Methods are readily available in the art for the hybridization of nucleic acid sequences. Coding sequences from other plants may be isolated according to well known techniques based on their sequence homology to the maize coding sequences set forth herein. In these techniques all or part of the known coding sequence is used as a probe which selectively hybridizes to other cell death-suppressor coding sequences present in a population of cloned genomic DNA fragments or cDNA fragments (i.e. genomic or cDNA libraries) from a chosen organism. For example, the entire llsl sequence or portions thereof may be used as probes capable of specifically hybridizing to corresponding coding sequences and messenger RNAs . To achieve specific hybridization under a variety of conditions, such probes include sequences that are unique among llsl coding sequences and are preferably at least about 10 nucleotides in length, and most preferably at least about 20 nucleotides in length. Such probes may be used to amplify llsl coding sequences from a chosen organism by the well -know process of polymerase chain reaction (PCR) . This technique may be used to isolate additional llsl coding sequences from a desired organism or as a diagnostic assay to determine the presence of llsl coding sequences in an organism. Such techniques include hybridization screening of plated DNA libraries (either plaques or colonies; see, e.g.. Sambrook et al . , Molecular Cloning, eds., Cold Spring Harbor Laboratory Press (1989) ) and amplification by PCR using oligonucleotide primers corresponding to sequence domains conserved among the amino acid sequences (see, e.gr. Innis et al . , PCR Protocols , a Guide to Methods and Applications, eds., Academic Press (1990)). For example, hybridization of such sequences may be carried out under conditions of reduced stringency, medium stringency or even stringent conditions (e.g., conditions represented by a wash stringency of 35-40% Formamide with 5x Denhardt's solution, 0.5% SDS and lx SSPE at 37° C; conditions represented by a wash stringency of 40-45% Formamide with 5x Denhardt's solution, 0.5% SDS, and lx SSPE at 42° C; and conditions represented by a wash stringency of 50% Formamide with 5x Denhardt's solution, 0.5% SDS and lx SSPE at 42° C, respectively) , to DNA encoding the cell death suppressor genes disclosed herein in a standard hybridization assay. See J. Sambrook et al . , Molecular Cloning, A Labora tory Manual 2d Ed . (1989) Cold Spring Harbor Laboratory. In general, sequences which code for a cell death suppressor and disease resistance protein and hybridize to the maize llsl gene disclosed herein will be at least 50% homologous, 70% homologous, and even 85% homologous or more with the maize sequence. That is, the sequence similarity of sequences may range, sharing at least about 50%, about 70%, and even about 85% sequence similarity. Generally, since leader peptides are not highly conserved between monocots and dicots, sequences can be utilized from the carboxyterminal end of the protein as probes for the isolation of corresponding sequences from any plant. Nucleotide probes can be constructed and utilized in hybridization experiments as discussed above. In this manner, even gene sequences which are divergent in the aminoterminal region can be identified and isolated for use in the methods of the invention. Also provided are mutant forms of the llsl gene (the cell death suppressor and disease resistance gene) and the proteins they encode. Methods for mutagenesis and nucleotide sequence alterations are well known in the art. See, for example, Kunkel, T. (1985) Proc . Na tl . Acad . Sci . USA 82:488-492; Kunkel et al . (1987) Methods in Enzymol . 154:367-382; US Patent No. 4,873,192; Walker and Gaastra (eds.) Techniques in Molecular Biology, MacMillan Publishing Company, NY (1983) and the references cited therein. Thus, the genes and nucleotide sequences of the invention include both the naturally occurring sequences as well as mutant forms. Likewise, the proteins of the invention encompass both naturally occurring proteins as well as variations and modified forms thereof. The nucleotide sequences encoding the proteins or polypeptides of the invention are useful in the genetic manipulation of plants. In this manner, the genes of the invention are provided in expression cassettes for expression in the plant of interest. The cassette will include 5' and 3' regulatory sequences operably linked to the gene of interest. The cassette may additionally contain at least one additional gene to be cotransformed into the organism. Alternatively, the gene(s) of interest can be provided on another expression cassette. Where appropriate, the gene(s) may be optimized for increased expression in the transformed plant. Where bacterial ring-hydroxylating dioxygenases are used in the invention, they can be synthesized using plant preferred codons for improved expression. Methods are available in the art for synthesizing plant preferred genes. See, for example, U.S. Patent Nos. 5,380,831, 5,436, 391, and Murray et al . (1989) Nucleic Acids Res . 17:477-498 , herein incorporated by reference . The expression cassettes may additionally contain 5' leader sequences in the expression cassette construct . Such leader sequences can act to enhance translation. Translation leaders are known in the art and include: picornavirus leaders, for example, EMCV leader (Encephalomyocarditis 5' noncoding region) (Elroy-Stein, 0., Fuerst, T.R., and Moss, B. (1989) PNAS USA, 86 :6126-6130) ; potyvirus leaders, for example, TEV leader (Tobacco Etch Virus) (Allison et al . (1986); MDMV leader (Maize Dwarf Mosaic Virus); Virology, 154:9-20) , and human immunoglobulin heavy-chain binding protein (BiP) , (Macejak, D.G., and P. Sarnow (1991) Nature, 353:90-94; untranslated leader from the coat protein mRNA of alfalfa mosaic virus (AMV RNA 4) , (Jobling, S.A., and Gehrke, L., (1987) Nature, 325:622-625; tobacco mosaic virus leader (TMV) , (Gallie, D.R. et al . (1989) Molecular Biology of RNA, pages 237-256; and maize chlorotic mottle virus leader (MCMV) (Lommel, S.A. et al . (1991) Virology, 81:382-385). See also, Della-Cioppa et al . (1987) Plant Physiology, 84:965-968. Other methods known to enhance translation can also be utilized, for example, introns, and the like. In preparing the expression cassette, the various DNA fragments may be manipulated, so as to provide for the DNA sequences in the proper orientation and, as appropriate, in the proper reading frame. Towards this end, adapters or linkers may be employed to join the DNA fragments or other manipulations may be involved to provide for convenient restriction sites, removal of superfluous DNA, removal of restriction sites, or the like. For this purpose, in vi tro mutagenesis, primer repair, restriction, annealing, resection, ligation, PCR, or the like may be employed, where insertions, deletions or substitutions, e . g. transitions and transversions, may be involved. The compositions and methods of the present invention can be used to transform any plant. In this manner, genetically modified plants, plant cells, plant tissue, seed, and the like can be obtained. Transformation protocols may vary depending on the type of plant or plant cell, i.e. monocot or dicot, targeted for transformation. Suitable methods of transforming plant cells include microinjection (Crossway et al . (1986) Biotechniques 4:320-334), electroporation (Riggs et al . (1986) Proc . Natl . Acad . Sci . USA, 83 : 5602-5606 , Agrobacterium mediated transformation (Hinchee et al . (1988) Biotechnology, f5:915-921), direct gene transfer (Paszkowski et al . (1984) EMBO J. , 3 : 111 -2122. ) , and ballistic particle acceleration (see, for example, Sanford et al., U.S. Patent 4,945,050; and McCabe et al . (1988) Biotechnology, f5:923-926) . Also see, Weissinger et al . (1988) Annual Rev. Genet . , 22:421-477 ; Sanford et al . (1987) Particulate Science and Technology, 5:27-37 (onion); Christou et al . (1988) Plant Physiol . 81 :671-674 (soybean) ; McCabe et al . (1988) Bio /Technology , 6:923-926 (soybean); Datta et al . (1990) Biotechnology, 8 : 736-740 (rice) ; Klein et al . (1988) Proc . Na tl . Acad . Sci . USA, 85 : 4305-4309 (maize) ; Klein et al . (1988) Biotechnology, 6:559-563 (maize); Klein et al . (1988) Plant Physiol . , 91 : 440-444 (maize) ; Fromm et al . (1990) Biotechnology, 8:833-839; and Tomes et al . "Direct DNA transfer into intact plant cells via microprojectile bombardment" In: Gamborg and Phillips (Eds.) Plant Cell, Tissue and Organ Culture: Fundamental Methods, Springer- Verlag, Berlin (1995) ; Hooydaas-Van Slogteren & Hooykaas (1984) Na ture (London) , 311:763-764; Bytebier et al . (1987) Proc . Na tl . Acad. Sci . USA, 84:5345-5349 (Liliaceae) ; De Wet et al . (1985) In The Experimental Manipulation of Ovule Tissues, ed. G.P. Chapman et al . , pp. 197-209. Longman, NY (pollen); Kaeppler et al . (1990) Plant Cell Reports , 5:415- 418; and Kaeppler et al . (1992) Theor. Appl . Genet . , 84:560- 566 (whisker-mediated transformation); D'Halluin et al . (1992) Plant Cell , 4:1495-1505 (electroporation); Li et al . (1993) Plant Cell Reports, 12:250-255 and Christou and Ford (1995) Annals of Botany, 75:407-413 (rice); Osjoda et al . (1996) Nature Biotechnology, 14:745-750 (maize via Agrobacterium tumefaciens) ; all of which are herein incorporated by reference. The cells which have been transformed may be grown into plants in accordance with conventional ways. See, for example, McCormick et al . (1986) Plant Cell Reports , 5:81- 84. These plants may then be grown, and either pollinated with the same transformed strain or different strains, and the resulting hybrid having the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that the subject phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure the desired phenotype or other property has been achieved. As noted earlier, the nucleotide sequences of the invention can be utilized to protect plants from disease, particularly those caused by plant pathogens. Pathogens of the invention include, but are not limited to, viruses or viroids, bacteria, insects, fungi, and the like. Viruses include tobacco or cucumber mosaic virus, ringspot virus, necrosis virus, maize dwarf mosaic virus, etc. Specific fungal pathogens for the major crops include: Soybeans : Phytophthora megasperma fsp. glycinea , Macrophomina phaseolina, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum, Diaporthe phaseolorum var . sojae (Phomopsis sojae), Diaporthe phaseolorum var. caulivora, Sclerotium rolfsii, Cercospora kikuchii , Cercospora sojina, Peronospora manshurica, Colletotrichum dematium (Colletotichum truncatum) , Corynespora cassiicola, Septoria glycines, Phyllosticta sojicola, Alternaria alternata, Pseudomonas syringae p.v. glycinea, Xanthomonas campestris p.v. phaseoli, Microsphaera diffusa, Fusarium semitectum, Phialophora gregata, Soybean mosaic virus, Glomerella glycines , Tobacco Ring spot virus, Tobacco Streak virus, Phakopsora pachyrhizi , Pythium aphanidermatum, Pythium ultimum, Pythium debaryanum, Tomato spotted wilt virus, Heterodera glycines Fusarium solani; Canola : Albugo Candida, Alternaria brassicae , Leptosphaeria maculans , Rhizoctonia solani, Sclerotinia sclerotiorum, Mycosphaerella brassiccola, Pythium ultimum, Peronospora parasitica, Fusarium roseum, Alternaria alternata; Alfalfa: Clavibater michiganese subsp. insidiosum, Pythium ultimum, Pythium irregulare, Pythium splendens , Pythium debaryanum, Pythium aphanidermatum, Phytophthora megasperma, Peronospora trifoliorum, Phoma medicaginis var. medicaginis , Cercospora medicaginis, Pseudopeziza medicaginis , Leptotrochila medicaginis , Fusarium oxysporum, Rhizoctonia solani, Uromyces striatus , Colletotrichum trifolii race 1 and race 2, Leptosphaerulina briosiana, Stemphylium botryosum, Stagonospora meliloti , Sclerotinia trifoliorum, Alfalfa Mosaic Virus, Verticillium albo-atrum, Xanthomonas campestris p.v. alfalfae, Aphanomyces euteiches , Stemphylium herbarum, Stemphylium alf lfae; Wheat : Pseudomonas syringae p.v. atrofaciens, Urocystis agropyri , Xanthomonas campestris p.v. translucens , Pseudomonas syringae p.v. syringae, Alternaria alternata, Cladosporium herbarum, Fusarium graminearum, Fusarium avenaceum, Fusarium culmorum, Ustilago tritici, Ascochyta tritici, Cephalosporium gramineum, Collotetrichum graminicola, Erysiphe graminis f . sp . tritici, Puccinia graminis f . sp . tritici, Puccinia recondita f . sp . tritici, Puccinia striiformis , Pyrenophora tritici-repentis , Septoria nodorum, Septoria tritici, Septoria avenae, Pseudocercosporella herpotrichoides , Rhizoctonia solani, Rhizoctonia cerealis , Gaeumannomyces graminis var. tritici, Pythium aphanidermatum, Pythium arrhenomanes , Pythium ultimum, Bipolaris sorokiniana, Barley Yellow Dwarf Virus, Brome Mosaic Virus, Soil Borne Wheat Mosaic Virus, Wheat Streak Mosaic Virus, Wheat Spindle Streak Virus, American Wheat Striate Virus, Claviceps purpurea, Tilletia tritici, Tilletia laevis, Ustilago tritici, Tilletia indica, Rhizoctonia solani, Pythium arrheno annes , Pythium gramicola, Pythium aphanidermatum, High Plains Virus, European wheat striate virus; Sunflower : Plasmophora halstedii , Sclerotinia sclerotiorum, Aster Yellows, Septoria helianthi, Phomopsis helianthi , Alternaria helianthi, Alternaria zinniae, Botrytis cinerea, Phoma macdonaldii , Macrophomina phaseolina, Erysiphe cichoracearum, Rhizopus oryzae , Rhizopus arrhizus , Rhizopus stolonifer, Puccinia helianthi , Verticillium dahliae, Erwinia carotovorum pv. carotovora, Cephalosporium acremonium, Phytophthora cryptogea, Albugo tragopogonis ; Corn: Fusarium moniliforme var. subglutinans , Erwinia stewartii , Fusarium moniliforme, Gibberella zeae (Fusarium graminearum) , Stenocarpella maydi (Diplodia maydis) , Pythium irregulare, Pythium debaryanum, Pythium graminicola, Pythium splendens , Pythium ultimum, Pythium aphanidermatum, Aspergillus flavus, Bipolaris maydis O, T (Cochliobolus heterostrophus) , Helminthosporium carbonum I, II & III (Cochliobolus carbonum) , Exserohilum turcicum I, II & III, Helminthosporium pedicel la turn, Physoderma maydis, Phyllosticta maydis, Kabatiella zeae, Colletotrichum graminicola, Cercospora zeae -maydis , Cercospora sorghi , Ustilago maydis, Puccinia sorghi, Puccinia polysora, Macrophomina phaseolina, Penicillium oxalicum, Nigrospora oryzae, Cladosporium herbarum, Curvularia lunata, Curvularia inaequalis , Curvularia pallescens , Clavibacter michiganense subsp . nebraskense, Trichoderma viride, Maize Dwarf Mosaic Virus A & B, Wheat Streak Mosaic Virus, Maize Chlorotic Dwarf Virus, Claviceps sorghi , Pseudonomas avenae, Erwinia chrysanthemi pv. zea, Erwinia corotovora , Cornstunt spiroplasma, Diplodia macrospora , Sclerophthora macrospora , Peronosclerospora sorghi , Peronosclerospora philippinensis , Peronosclerospora maydis, Peronosclerospora sacchari , Spacelotheca reiliana , Physopella zeae, Cephalosporiu maydis, Caphalosporium acremonium, Maize Chlorotic Mottle Virus, High Plains Virus, Maize Mosaic Virus, Maize Rayado Fino Virus, Maize Streak Virus, Maize Stripe Virus, Maize Rough Dwarf Virus; Sorghum: Exserohilum turcicum, Colletotrichum graminicola (Glo erella graminicola) , Cercospora sorghi , Gloeocercospora sorghi , Ascochyta sorghina, Pseudomonas syringae p.v. syringae, Xanthomonas campestris p.v. hol cicola , Pseudomonas andropogonis , Puccinia purpurea , Macrophomina phaseolina , Perconia circinata , Fusarium moniliforme, Al ternaria al ternate, Bipolaris sorghicola , Helminthosporium sorghicola , Curvularia luna ta , Phoma insidiosa , Pseudomonas avenae (Pseudomonas alboprecipi tans) , Ramulispora sorghi , Ramulispora sorghicola, Phyllachara sacchari , Sporisorium reilianum (Sphacelotheca reiliana) , Sphacelotheca cruenta , Sporisorium sorghi , Sugarcane mosaic H, Maize Dwarf Mosaic Virus A & B, Claviceps sorghi , Rhizoctonia solani , Acremonium strictum, Sclerophthona macrospora , Peronosclerospora sorghi , Peronosclerospora philippinensis , Sclerospora graminicola, Fusarium graminearum, Fusarium oxysporum, Pythium arrhenomanes , Pythium graminicola, etc. The nucleotide sequences also find use in enhancing transformation efficiency by suppressing cell death in bombarded cells. Thus, the sequences find particular use in transformation methods in which programmed cell death occurs. The physical wounding of particle bombardment triggers programmed cell death. The expression of the cell death- suppressor gene in a bombarded cell serves to inhibit such cell death thereby improving transformation efficiency. By "improving efficiency" is intended that the number of transformed plants recovered by a transformation event is increased. Generally, the number of transformed plants recovered is increased at least two-fold, preferably at least five-fold, more preferably at least ten-fold. For use in improving transformation efficiency, a cell death suppressor gene is included in an expression cassette. Typically, the gene will be used in combination with a marker gene. Other genes of interest may additionally be included. The respective genes may be contained in a single expression cassette, or alternatively in separate cassettes. Methods for construction of the cassettes and transformation methods have been described above . As noted, the cell death suppressor gene can be used in combination with a marker gene. Selectable marker genes and reporter genes are known in the art. See generally, G. T. Yarranton (1992) Curr. Opin . Biotech . , 3:506-511; Christopherson et al . (1992) Proc . Na tl . Acad . Sci . USA, 89:6314-6318; Yao et al . (1992) Cell , 71:63-72; W. S. Reznikoff (1992) Mol . Microbiol . , 6 : 2419-2422 ; Barkley et al . (1980) The Operon, pp. 177-220; Hu et al . (1987) Cell , 48:555-566; Brown et al . (1987) Cell , 49:603-612; Figge et al . (1988) Cell , 52:713-722; and, Deuschle et al . (1989) Proc . Na tl . Acad . Aci . USA, 86:5400-5404. Plant tissue cultures and recombinant plant cells containing the proteins and nucleotide sequences, or the purified protein, of the invention may also be used in an assay to screen chemicals whose targets have not been identified to determine if they inhibit llsl protein. Such an assay is useful as a general screen to identify chemicals which inhibit llsl protein activity and which are therefore herbicide candidates. Alternatively, recombinantly-produced llsl protein may be used to elucidate the complex structure of the enzyme. Such information regarding the structure of the llsl protein may be used, for example, in the rational design of new inhibitory herbicides. It is recognized that both plant and bacterial nucleotide sequences may be utilized. The inhibitory effect on the cell-suppressor protein may be determined in an assay by monitoring the rate of cell death or alternatively by monitoring the accumulation of the activating phenolic compound, particularly the para-coumaric ester associated with lesion mutants . If such a chemical is found, it would be useful as a herbicide, particularly if plant or bacterial mutant genes can be isolated or constructed which are not inhibited by the chemical. As indicated above, molecular techniques are available in the art for the mutagenesis and alteration of nucleotide sequences. Those sequences of interest can be selected based on resistance to the chemical . Where resistant forms of llsl ox a corresponding gene have been identified to a chemical, the chemical is also useful as a selection agent in transformation experiments. In these instances, the mutant llsl would be used as the selectable marker gene . The sequences of the invention also find use to genetically target cell ablations. In this manner, dominant negative nucleotide sequences can be utilized for cell ablation by expressing such negative sequences with specific tissue promoters. See Figure 3 and SEQ ID NOS 2 & 5-61, respectively. For example, stamen promoters can be utilized to drive the negative alleles to achieve male sterile plants. (See, for example, EPA0344029 and U.S. Patent No. 5,470,359, herein incorporated by reference) . Alternatively, cell ablation can be obtained by disrupting dominant negative oligonucleotides with a transposable insertion. In this manner, very specific or general patterns of cell ablations can be created. Additionally, to provide specific cell ablation, antisense oligonucleotides for llsl or other genes of the invention can be expressed in target cells disrupting the translation which produces the cell death suppressor proteins. As discussed, the genes of the invention can be manipulated to enhance disease resistance in plants. In this manner, the expression or activity of the llsl or other cell death suppressor or disease resistance gene can be altered. Such means for alteration of the gene include co- suppression, antisense, mutagenesis, alteration of the sub- cellular localization of the protein, etc. In some instances, it may be beneficial to express the gene from an inducible promoter, particularly from a pathogen inducible promoter. Such promoters include those from pathogenesis- related proteins (PR proteins) which are induced following infection by a pathogen; e.g., PR proteins, SAR proteins, beta-1 , 3-glucanase, chitinase, etc. See, for example, Redolfi et al . (1983) Neth . J. Plant Pathol . 89:245-254; Uknes et al . (1992) The Plant Cell 4:645-656; and Van Loon (1985) Plant Mol . Virol . 4:111-116. A promoter which is capable of driving the expression of genes in a plant cell is additionally provided. The promoter is inducible. Thus, it may be manipulated to express heterologous resistance mechanisms at the site of pathogen infection. Accordingly, the promoter is useful for driving any gene in a plant cell, particularly genes which are needed at the site of infection or wounding. That is, the promoter is particularly useful for driving the expression of disease or insect resistance genes. The nucleotide sequence of the promoter is provided in SEQ ID NO: 3. It is recognized that the nucleotide sequence of the promoter may be manipulated yet still retain the functional activity. Such methods for manipulation include those discussed above. Thus, the invention encompasses those modified promoter sequences, as well as promoter elements retaining the functional activity of the promoter. Such elements and modified sequences can be assayed for activity by determining the expression of a reporter gene operably linked to the promoter element or modified promoter sequence . A genomic DNA sequence comprising the 11s gene and promoter are provided in SEQ ID NO: 4. The sequence can be used to construct probes to determine the location and organization of similar sequences in other plants, particularly to analyze the location of other cell death suppressing and disease resistance sequences. The following examples are offered by way of illustration and not by way of limitation. Experimental Materials and Methods Plant material The original llsl mutant, containing the reference allele, was obtained from the Maize Genetics Coop., University of Illinois, Urbana/Champaign. Stocks containing active Mu transposons were obtained from Dr. D. Robertson, Iowa State University. The six transposon tagged mutant alleles, llsl -1 through llsl - 6, were previously designated as 11 B* - 29215 , 21s*-42230, 11s* - 1127 , Ils*-1424, 12s*-3744, and lls*-4911, respectively (Johal et al . , (1994), A Tale of Two Mimics; Transposon Mutagenesis and characterization of Two Disease Lesion Mimic Mutations of Maize, Maydica 39:69- 76) . DNA extraction, RFLP mapping and co-segregation analysis DNA was isolated by a urea (Dellaporta et al . (1983), Plant Molecular Biology Reporter 1:19-22) or CTAB (Hulbert et al . (1991) Molecular and General Genetics 226:377-382) extraction protocol. DNA samples (15 to 30) from either mutant or wild-type plants were pooled and digested individually with seven restriction enzymes. Southern blot analysis was performed as described by (Gardiner et al . (1993) Genetics 134:917-930) except that UV crosslinking and use of dextran sulfate were omitted. Blots were hybridized systematically with specific probes from different Mu elements. Mapping probes were provided either by the Maize Mapping Project at the University of Missouri or from Pioneer Hi-Bred Int. Inc. Pre-hybridizations and hybridization of southern blots was performed at 65 °C unless otherwise specified. A 3.0 kb EcorRI Mu8co-segregating DNA marker was cloned from an llsl * -5/llsl -ref plant using standard cloning procedures (Ausubel et al . (1994) Current Protocols in Molecular Biology) . The Zap Express™ vector (Stratagene) was employed and packaging, screening and in vivo excision protocols performed according to manufacturers instructions. The primer sequences (SEQ ID NOS 62-64, respectively) for confirmation analysis were: GSP1 : 5' TGG GGA ACT TGA TCG CGC ACG CCT TCG G3 ' , GSP2 : 5' TCG GGC ATG GCC TGG GGG ATC TTG G 3', and GSP3 : 5' GGC CAC GCG TCG ACT AGT AC 3' (IDT, Coralville IA) . The thermocycling regime used for confirmation analysis was 94 °C for 5 min, then cycled 40 or 42 times for 30 seconds at 94 °C, 1 min and 30 sec at 62 °C, and I min at 72 °C, and finally 5 min at 72 °C. Mini-libraries of cloned amplified fragments using the TA Cloning4S vector (Invitrogen) were created and individual colonies for clones with inserts of the appropriate size. A 5' RACE fragment was used to screen a pa405 maize seedling leaf cDNA library and 3 individual clones were recovered and converted to the phagemid form by in vivo excision from the Zap Express™ (Stratagene) vector. Primers GSP1 and GSP2 were used for 5' RACE and GSP3 was used for 3' RACE using 5' and 3' RACE Kits and recommended manufacturers instructions (GIBCO, MD) . To isolate an llsl genomic clone, a B73 partial SauIIIA library in lambda Dashll was screened using a probe from a 3' RACE product spanning the llsl gene from GSP3 to the polyadenylation site. A single positive clone was recovered and a 7.129 kb Sacl fragment was subcloned into pBSKS÷ (Stratagene) to create the plasmid pJG201. RFLP mapping of the Arabidopsis llsl homolog was performed using the Recombinant Inbred (RI) lines generated from a cross between Arabidopsis ecotypes Columbia and Landsberg erecta. 48 RI lines were scored using an EcoRV polymorphism using an llsl homolog cDNA as probe. The map position was determined on MAPMAKER using the Kosambi mapping function (Lander et al . (1987) Genetics 121:174-181) . Primer extension analysis For primer extension analysis of the maize llsl gene an oligonucleotide complementary to the coding strand in the llsl gene from 139-173 bases downstream of the predicted first in- frame ATG was synthesized by DNA Technologies, Inc. (Coralville, IA) . The oligonucleotide (SEQ ID NO: 65) GSP17 (5' GTG CTC GGC TCC GCC TGC TCC GCC GCT TCC CCT GG 3 ' ) was end- labeled with 32 P. Primer extension analysis was performed by the method described by McKnight et al . (1981), Analysis of Transcriptional Regulatory Signals of the HSV Thymidine Kinase Gene : Identifica tin of an Upstream Control Region, Cell 25:385-398, except for the following modifications. 40 mg of total RNA from immature tassels of a B73 inbred plant and 0.2 pmol of labeled oligonucleotide were annealed at one of either 33 °C, 37 °C, 45 °C, or 55 °C for 4 hours. Following the extension reaction RNA in the sample was removed by adding 2ul of 0.5M EDTA and lul of mixed RNAases (0.5 mg/ml RNAase A and 10,000 units/ml RNase Tl; Ambion) and incubating at 37°C for 30 minutes. The primer extension products were separated on a 6% denaturing polyacrylarnide sequencing gel and the size of the extension product determined by comparison with a DNA sequence ladder. Northern blot analysis Total RNA was isolated from leaves of 10 leaf-stage wild-type plants in a population segregating for the LeslOl mutation, Johal and Briggs (1992) Science 258 : 985-987. mRNA was enriched from total RNA using a magnetic bead affinity protocol (Dynal Inc. Great Nect NY) . mRNA was isolated from A632 inbred plants using the MicroQuick protocol (Pharmacia, Piscataway NJ) . Hybridizations were performed either in modified Church and Gilberts solution at 42 °C or in the following hybridization solution at 65 °C - 1% casein (Technical Grade, Sigma), 1% calf skin gelatin (225 bloom, Sigma), 0.2% SDS (Mol. Biol Grade, Fisher), 0.1% Sarkosyl (IBI) , 5XSSC. Transfer to nylon membrane (Magnacharge MSI, Westboro MA) was performed by standard protocols, hybridizations were carried out overnight and blots were washed as indicated in the results section. DNA sequencing and analysis DNA sequencing was performed by a cycle sequence method using a SequiTherm™ Cycle Sequencing Kit (Epicentre, Madison WI . ) according to the manufacturers protocol . Local sequence comparisons were performed using software including ALIGN and MEGALIGN programs of the DNASTAR software package (DNASTAR Inc. Madison WI) . Algorithms such as the neighborhood search algorithm BLAST (Autschul et al . (1990), Basic Local Alignment Search Tool , J. Mol. Biol. 215:403- 410) or BEAUTY (Worley et al . (1995), An Enhanced BLAST- based Search Tool that Integrates Mul tiple Biologi cal Informa tion Resources into Sequence Similari ty Search Resul ts, Genome Res . 5:173-184) were employed. Searches of the Genbank databases were performed using the National Center for Biotechnology Information's BLAST WWW Server with links to Entrez and to the Sequence Retrieval System (SRS) provided by the Human Genome Center, Baylor College of Medicine Server at Houston Texas via Internet access. Analysis of light requirement for llsl and dd lesion development To determine the spectral range of light required for lesion formation, sections of leaves were clamped between 0.125 inch Plexiglas GM filters held in place by a metal stand with a side arm clamp. The following transparent filters were used: Plexiglas GM 2423 (red) , 2711 (Far red) , 2424 (blue) , 2092 (green) , 2208 (yellow) , and 2422 (Amber) or Clear, (Cope Plastics Inc. St. Louis. MO). Transmission spectra of filters were determined by examining small sections of filters in a spectrophotometer . Leaf sections of greenhouse or field-grown plants were covered in aluminum foil to completely remove incident light. Following complete lesioning of a leaf, filters were removed to observe if lesioning had occurred in the covered region. The llsl mutation is cell autonomous and llsl lesions exhibit altered phenolic metabolism and callose formation. The expression of the llsl phenotype is developmentally programmed: a number of round to elliptical lesions often with concentric rings of dead and dying tissue, begin as small chlorotic flecks near the tip of the first leaf at the three to four leaf stage. While these lesions continue to enlarge and eventually coalesce, new lesions initiate down the leafblade along an age gradient and cover the whole leaf within three to four days. Meanwhile, lesions have already started near the tip of the second leaf. This pattern continues and the plant dies shortly after pollen shed. Although the entire leaf tissue becomes necrotic on llsl plants, lesions rarely develop on stalks. The llsl mutation is cell autonomous (i.e., the effect of the gene is confined to the cell in which it is expressed) as exhibited by both revertant sectors (Johal et al . (1994) Maydica , 69-76) and forward sectors in that the mutant phenotype does not progress into surrounding wild-type tissue. Llsl lesions were examined for callose deposition which is frequently associated with response to pathogen infection, wounding or intercellular viral movement (Hammond-Kosack et al . (1996), Resistance Gene -dependent Plant Defense Responses, Plant Cell 8:1773-1791). Heavy callousing of all cell types within lesions was observed. At the edge of lesions where cells had not yet collapsed, individual bundle sheath cells were the first cells to exhibit callousing of the plasmodesmatal fields suggesting that the cells were responding to some factor or signal emanating from the dying or dead cells. Mapping of the llsl locus. The original llsl allele isolated by Ullstrup and Troyer (Ullstrup et al . (1967) Phytopa thology 57:1282-1283) was used as the reference allele (llsl -ref) . Using a combination of cytogenetic and genetic methods, the llsl gene was initially mapped to the short arm of chromosome 1 (IS) (Hoisington, (1984) Maize Genetics Newsletter 58:82- 84) . To map the gene at a higher resolution, a new population, in which the progeny segregated 1:1 for homozygous and heterozygous llsl plants, was generated. A W23 inbred plant was fertilized with the llsl pollen derived from an llsl -ref /llsl -ref plant, and the resulting progeny (two plants) were backcrossed with the llsl -ref homozygotes . DNA isolated from 16 mutant and 14 wild-type plants was used to examine the linkage with a number of RFLP markers. Three tightly linked RFLP markers were identified which flank the llsl locus. The RFLP marker Php200603 is about 5cm distal to llsl , whereas UMC157 is about 8cm proximal to llsl . The linkage of llsl with another marker, Php200689, could not be broken with these 30 DNAs . All three of these RFLP markers were invaluable in unequivocally classifying the mutant alleles for co-segregation analyses. Cloning of the llsl locus by transposon tagging. Due to the lack of biochemical information on the llsl mutation, a transposon tagging method was employed to clone the llsl gene. This experimental approach allows genes to be cloned solely on the basis of phenotype (Bennetzen et al . (1987) , Proceedings of the UCLA Symposium : Plant Gene Systems and their Biology, ed, 183-204) . Both targeted and non-targeted approaches were employed as outlined by (Johal et al . (1994) Maydica, 69-76). For the targeted approach, llsl -ref / llsl -ref plants were used as male parents and crossed with wild-type plants (Llsl/Llsl) from lines active for Mu transposition. All Fl plants were expected to be of wild-type phenotype (Llsl/ llsl -ref ) unless a Mu insertion or some other mechanism had inactivated the Llsl allele. Such an event would result in an llsl */ llsl -ref plant (llsl * refers to a mutant allele generated during transposon tagging) with a mutant phenotype. Three plants from approximately 30,000 Fl progeny exhibited the mutant phenotype and one of these died before shedding any pollen. The remaining two plants were crossed as male parents to B73 and Prl inbreds and these two new mutants have been designated llsl * -l and llsl * -2 (lls* -29215 and lls* -42230 , respectively, in (Johal et al . (1994) Maydica , 69-76). A few of the progeny (10 plants) from the outcross of the mutant plants with both inbreds were RFLP genotyped to identify plants which had inherited the mutant allele (llsl *) . Two plants containing the mutant allele were self-fertilized, and the F2 progeny so derived were found to segregate for the llsl phenotype in a 1:3 ratio as expected for a recessive mutation. Two other mutant allele-containing plants from the outcross progeny were backcrossed with the llsl -ref /llslref mutants. The resultant progeny segregated 1:1 for mutant (llsl * -l or -2111sl -ref) versus normal plants (Llsl -B73 or -Prl/llsl -ref) and were used for co-segregation analysis. For non-targeted mutagenesis, Mu-active stocks were crossed to an inbred line and the resulting progeny was self-pollinated to generate F2 (M2) Mutator populations. With this approach, any recessive mutation generated during the Fl cross can be detected in the F2 generation. From more than 24,000 Mutator F2 families screened, four independent families were identified in which one- fourth of the plants exhibited a phenotype typical of llsl . The four mutant alleles have been designated llsl* - 3 , llsl *4 , llsl * -5 and llsl * - 6. A number of wild-type plants from each of these four families were pollinated with the llsl - ref /llsl -ref pollen to determine allelism between these new l sl-like mutants and the original llsl mutant. The segregation of llsl mutants in the progeny of most of these crosses confirmed allelism between llsl and the new mutants. All of these mutants were outcrossed with B73 twice and backcrossed to the llslref /llsl -ref mutant to create populations suitable for co-segregation analysis as described above for the targeted mutants. The next step was to confirm that the Mu elements (there are at least nine of them for Mutator) had caused these new insertional mutations. This step, called co- segregation analysis, involved Southern blot analysis to detect the linkage of a Mu element with the mutant allele in question (Bennetzen et al . (1993) Specifici ty and Regula tion of the Mutator Transposable Element System in Maize, Crit . Rev. Plant Sci. 12:57-95) . DNA was isolated from phenotypically mutant and wild-type plants from the segregating populations described above for each of the mutant alleles. Following identification of a putative co-segregating element, the analysis was extended employing multiple individual DNA samples digested with an appropriate restriction enzyme. In this manner a 3kb EcoRI restriction fragment, hybridizing with the Mu8 specific probe was found to co-segregate with 66 DNA samples from the llsl * -5 mutation. This co-segregating fragment was cloned and sequenced revealing the organization indicated in Figure 1. The DNA sequence of the right (267bp) flank exhibited significant homology with an Arabidopsis EST of unknown function suggesting that an actual gene was disrupted by the Mu8 insertion. On sequencing the 1344 bp left flanking DNA no significant homology to known DNA sequences was detected and a Mu TIR DNA junction (terminal inverted repeats at each end of Mu elements) was not observed. Using a Mu TIR primer and either an M13 forward or reverse universal primer the left flanking (1344bp) or right flanking (267 bp) DNA was amplified by PCR and used to probe mutant and wild-type DNA samples of all mutant alleles. This experiment revealed single band polymorphisms in nearly all alleles suggesting that this locus was disrupted in several other alleles. The occurrence of insertions in the same locus for multiple alleles of the same mutation is considered proof that the correct locus has been tagged. A PCR based approach was used to identify Mu type insertions in the vicinity of the cloned region. The right flanking DNA from the llsl*-5 clone was sequenced as described above and primers designed for extension in each direction. These primers were used in combination with Mu TIR primers to detect amplification products in other mutant allele DNA samples but that were absent in their corresponding wild-type samples. Two such PCR polymorphisms were identified from the targeted allele llsl * -2 and the non-targeted allele llsl* . These products hybridized strongly on a southern blot with the right flanking DNA from allele llsl* - 5 indicating that these amplification products were amplified from the same locus. In addition, the amplification of a smaller (189bp) gene specific fragment was observed in all the mutant and wild-type DNA samples from all alleles that hybridized with the right flanking DNA of the original llsl * -5 clone. Since all these samples were heterozygous for the llsl -ref allele this result indicated that the llsl -ref mutation had also resulted from a Mu insertion. Nested PCR using a Mu TIR primer and GSP2 was performed to isolate this fragment. All PCR products were directly sequenced using the GSP1 or GSP2 primers as sequencing primer and allowed identification of Mu-type insertions within 246 bp and 292 bp 5' of the insertion site of allele llsl * -5 in allele ll sl* - 2 and llsl * - A respectively. It was determined that the llsl -ref allele had a Mu insertion at the same site of insertion as that of allele llsl * -5. Southern analysis using the left-flanking DNA of the llsl* - 5 clone revealed that the insertion of a Mu element in the llsl -ref allele was not accompanied by a duplication event showing that the two alleles arose due to independent transposition events (explained below) . The occurrence of four independent Mutator insertions in the same locus in plants with the llsl phenotype but not their corresponding wild-type siblings constitutes proof that a fragment of the llsl locus had been isolated. It was observed that a Mu insertion event gave rise to the llsl -ref allele which was believed to arise in a non-Mu active background, suggesting that cosegregation analysis should be attempted with an allele of unknown origin before employing it in a targeted mutagenesis strategy since the occurrence of an insertion in the same region of the gene could obfuscate co-segregation analysis with a new allele. The llsl locus encodes a novel plant protein To characterize the llsl locus fully a cDNA and genomic clone was isolated. Gene specific primers GSP1 and GSP3 were employed along with universal primers to amplify 5' and 3' fragments respectively of the llsl transcript from a cDNA library constructed from 2 week old inbred PA405 seedlings. A 5' fragment was then used as a probe to screen the PA405 cDNA library and 3 individual clones were recovered and the longest phagemid named pJG200 was sequenced (Genbank Ace. # U77345) . This sequence was used to screen a maize EST database and another llsl cDNA with an additional 180 bp at the 5' end was recovered. The combined sequence of these two cDNAs is shown in SEQ ID NO : 1 and a 521 amino acid continuous open reading frame can be predicted from this partial transcript (SEQ ID NOS 1 & 2, respectively) . The identification of the termination codon was supported by a similarly located predicted termination codon in the sequence of an Arabidopsis llsl homolog (below) . A primer designed against 139bp to 173bp downstream of the predicted start codon of this sequence (GSP 17) was used for primer extension analysis and a 454 bp long primer extension product was observed thus predicting a 2119 bp total length transcript for the llsl gene. In addition, the 3' ends of the cDNAs and the 3' ends of the three PCR-amplified 3 '-ends were also sequenced and three different polyadenylation sites determined thus allowing for variation in the size of the full length transcript (SEQ ID NO: 1 and Figure 1) . A 3' fragment of the llsl gene was utilized to screen a partial Sau3A genomic library of the maize inbred line B73 in order to isolate a full-length llsl gene sequence and a single positive clone (designated G18) was isolated. A 7129 bp Sacl fragment was subcloned from the G18 genomic clone and the resulting plasmid named pJG201 was entirely sequenced (Genbank Acc# U77346) . By comparison with the cDNA sequence pJG201 was found to contain almost the entire llsl coding region and a 5' region likely to include the entire promoter. The predicted genomic organization of the llsl gene (Figure 1) includes 7 exons and 6 introns . The Sacl restriction site at bp 7129 is 45 bp upstream of the predicted stop codon and 320bp upstream of the polyadenylation sites. Providing that there are no other introns in the 5' region of the gene the predicted transcriptional start site of the llsl gene occurs at bp 3115 of the 7129 bp subclone. Southern hybridization suggests that the llsl gene is single copy in the genome of maize since only one band was observed on Southern blots of the wild-type DNA samples of the llsl-ref allele cut with several restriction enzymes. That a duplicate of the llsl gene exists has not yet been determined using lower stringency washes. Three bands were observed in llsl* - 5 when the EcoRl digested mutant samples were probed with the left flank. A 10 bp direct repeat was not observed on each side of the Mu8 insertion in allele llsl *5 . These results suggested that a rearrangement of DNA more complex than a simple Mu8 element insertion had occurred at this locus and the nature of this rearrangement was determined by comparison with the genomic sequence of the llsl gene. The left flanking DNA comprises a direct repeat of the llsl genomic sequence extending from the EcoRl site of Intron II to bp 43 of exon 4. The predicted llsl protein exhibits a largely hydrophilic protein with a pi of 7.5. No hydrophobic regions suggesting membrane association were observed. This fact suggests a cytosolic or plastidic subcellular location for the LLS 1 protein. The llsl gene exhibits tissue and cell specific expression The llsl phenotype is developmentally expressed as described above. LLS1 appears to be needed in expanded leaves but not in very young leaves and thus llsl transcripts may accumulate in older leaves if the gene is transcriptionally regulated. The expression of llsl in fully expanded leaves of normal plants was examined using a partial cDNA probe that extends from the beginning of exon 2 to the end of the llsl transcript. A weak signal was detected using 20pg of total RNA and a high stringency wash. There did not appear to be a significant gradient in gene expression from three successively older leaves. When mRNA derived from pooled total RNA from these leaves was utilized a single transcript was readily detected (Figure 2) . The size of this single transcript was estimated at 1.9 ± 0.2 kb a figure which coincides closely with the full-length size determined by primer extension analysis (1.129 kb) . To further examine the developmental pattern of llsl gene expression, mRNA derived from various plant tissues was probed with an 802bp Notl/Pstl fragment that extends from the end of exon 2 to exon 7 (Figure 1) . Lowest levels of expression were seen in seedling leaves, 3 week old embryos and in young tassels. The llsl transcript was readily detected in more mature tassels, young and old ear shoots and 1 week old embryoe . Surprisingly, the llsl transcript was most readily detected in seedling roots where the llsl phenotype has not been observed. In addition, the presence of a second larger transcript (approximately 2.4 kb) was observed that hybridizes with the llsl probe in seedling roots and older tassel tissue. When observed this larger transcript seems to be expressed in equivalent amounts to the lower transcript. Since genomic blots have indicated that llsl is a single copy gene, the larger transcript may represent post-transcriptional regulation of llsl although there is precedence for a northern blot to reveal the existence of a second gene when a southern analysis failed to do so. These results indicate that the llsl gene is not expressed constitutively in all tissues but exhibits tissue specific transcriptional regulation. The llsl gene is conserved between monocot and dicot plants To determine if llsl related genes are present in other species or organisms the predicted llsl protein sequence was utilized to search public databases of sequences of both known and unknown functions. As indicated above, significant homology (70% nucleic acid identity) was observed between the right flanking DNA of llsl* - 5 and an expressed sequence tag (EST) from Arabidopsis thaliana . (Genbank Ace. # T45298) . Three other Arabidopsis ESTs were identified that overlap with this EST (Genbank Ace. #s N37395, H36617 and R30609) . The four overlapping ESTs were obtained from the ABRC (Columbus, OH) and further sequenced. These sequences were organized into a single contig 1977 bp in length (Genbank Ace. # U77347) . The 3 ' end of this contig overlaps with the upstream region of the rpl9 gene (a nuclear encoded plastid ribosomal protein) ending only 109 bp upstream of the rpl9 transcriptional start. The Arabidopsis contig that exhibits 71.6% amino acid similarity over a 473 consensus length with the maize 11s ORF from the available maize cDNA sequence. The amino terminus of the maize versus the Arabidopsis ORFs differ significantly indicating the possibility that each protein has a different leader peptide or that an alterative start codon is utilized. The maize llsl sequence has therefore been utilized to detect a highly homologous gene from a dicot plant. This result prompted us to map the Arabidopsis contig and this was achieved using the recombinant Inbred (RI) lines developed by Clare Lister and Caroline Dean at the John Innes Center (Lister et al . (1993) Plant Journal 4:745-750). Following identification of a suitable polymorphism one EST (Acc# T45298) was used as a probe to score 48 RI lines. The map position was located on the lower arm of chromosome three between GL1 and m249. Importantly, the acdl mutation, whose cell death phenotype is reminiscent of the maize llsl , also maps in this region (Greenberg et al . (1993) Arabidopsis Mutants Compromised for the Control of Cellular Damage During Pathogenesis and Aging, Plant J. 4:327-341) suggesting that these two mutations in maize and Arabidopsis are homologous. As genomes from two divergent plant species have been found to have related llsl genes, it is likely that any number of plant species will possess genes regulating cell survival in a manner similar to the maize llsl gene. To further test this hypothesis we tested the linkage of maize llsl and flanking markers to a sorghum mutation named drop-dead-1 (dd-1 ) that is an EMS induced lesion-mimic mutation with spreading lesions highly reminiscent of llsl lesions. A segregating mapping population was created by crossing a dd/dd line with Shangai Red DD/DD and the progeny were allowed to self. Plants segregating for drop-dead were identified and DNA isolated from several mutant and wild-type progeny. A polymorphism for the llsl locus could not be identified but a polymorphism for the probe PIO200640 which is ~33cM distal to llsl was identified with HindE . This polymorphism showed complete segregation with 14 mutant and 16 wild-type progeny strongly suggesting that this mutation maps to a region syntenic with llsl and that llsl and dd are homologous mutations and possibly orthologs . llsl lesions are induced by wounding and in lesl Ol/llsl double mutants In addition to intrinsic, developmental signals, external factors also affect llsl expression. llsl lesions normally appear randomly on developmentally competent areas of the leaf. However, llsl lesions can also be triggered to initiate at any site (provided that the tissue is developmentally competent) by killing cells either by inducing an HR with an incompatible pathogen or by physical means (making pin prick wounds) . The additive phenotype of the double mutant of llsl with Les2 or Les* -101 (two dominant mimics that can initiate numerous lesions on maize leaves before they become developmentally competent to express llsl) further supports these results. On the double mutants, the early phenotype of the lesions is discrete and of the respective Les type and also of higher density as compared to that of llsl lesions. However, as the tissue acquires developmental competence to be able to express the llsl phenotype, most, if not all, Les sites transform into llsl lesions that expand in an uncontrolled fashion to consume the whole leaf. Thus the internal metabolic upset and cell death events associated with a Les* -101 lesion appear to act as a trigger for llsl lesions. Light is required for llsl and dd lesion formation These observations fully support the hypothesis that llsl functions to contain cell death from spreading, and it appears to be critical during late stages of plant development. Interestingly, the expression of llsl lesions is completely dependent on light. The region in the center of the leaf was covered with aluminum foil just as lesions were initiating at the tip of the leaf. The lesions formed progressively down the leaf but not where the leaf was protected from light. Aluminum foil also protected lesions induced by pin-prick wounding in maize llsl plants and also observed clearly in sorghum drop-dead plants. Using plastic filters that transmit different wavelengths of light, it was found that visible light in the spectral region of 650-700 nm is sufficient for this effect. Yellow and orange filters also transmitted some red light in the 650-700 nm so a contribution from light in the 560 to 640 nm range cannot be excluded. Lesions did not form when only blue, green, or far-red light reached the leaf. This phenomenon suggested that active photosynthesis, which harvests light pre-dominantly in the red spectral region, is required for lesion formation. This was addressed genetically by creating double mutants of llsl with iojap 1 (ijl - a recessive mutation in maize that produces albino and light green sectors on an otherwise normal green leaf) or ncsl which also exhibits light green but not albino sectors. These double mutants have revealed that llsl lesions can only form in dark green tissues. This result indicates that some activity related to light harvest or photosynthesis may be important in the initiation and spread of lesions. Double mutants of llsl with oil yellow- 700 provide further support to this interpretation. Oyl - is a dominant mutation which by virtue of its inability to convert protoporphyrin IX to Mg-protoporphyrin, is completely devoid of chlorophyll b and has also reduced levels of chlorophyll a. On oy/+ llsl/llsl plants lesions initiate with a lower density and propagate very slowly in these plants and often lethality does not ensue. Intriguingly, the suppressible effect of oyl on llsl is not observed when the plants are grown in a greenhouse or growth chamber. Also we have observed that on an llsl/ ijl double mutant, where lesions do not initiate or develop in albino tissue, the 'death' signal (that probably allows llsl lesions to propagate) can sometimes diffuse across (traverse) the albino tissue if the sector is narrow. This suppression is in contrast with many other lesion mimics such as the dominant lesion mimic Les4 which readily forms lesions in the albino sectors of Les4/+ ij/ij plants. These observations indicate that a process or a metabolite, which is partly diffusible and whose activity may be affected by factors including light, wounding, and pathogen invasion, is responsible for the initiation and spread of cell death associated with llsl lesions. The predicted LLS1 protein contains two structural motifs highly conserved in baterial phenolic dioxygenases While no definite function could be ascribed to llsl from homology searches, analysis of the predicted amino acid sequence of the llsl gene product has revealed two conserved motifs, a consensus sequence (SEQ ID NO: 6) (Cys-X-His-X 16 _ 17 - Cis-X 2 -His) for coordinating the Reiske-type [2Fe-2S] cluster (Mason and Cammock (1992) The Electron - Trnasport Proteins of Hydroxylating Bacterial Dioxygenases , Annu. Rev. Microbiol. 46:277-305) and a conserved mononuclear non-heme Fe-binding site (SEQ ID NO: 7) (Glu-X 3 _ 4 -Asp-X2 -His-X 4 _ 5 -His) (Jiang et al . (1996) Si te -directed Mutagenesis of Conserved Amino Acids in the Alpha Subuni t of Toluene Dioxygenase : Potential Mononuclear Nonheme Iron Coordination Si tes , J Bacteriol. 178:3133-3139), which are present in the a- subunit of all aromatic ring-hydroxylating (ARH) dioxygenases involved in the degradation of phenolic hydrocarbons. In addition, the spacing (-90 amino acids) between these motifs, which has recently been shown to be conserved in all ARH dioxygenases, is precisely maintained in LLS1, adding further evidence that LLS1 may encode a dioxygenase function. The ARH dioxygenases consist of 2 or 3 soluble proteins that interact to form an electron transport chain that transfers electrons from NADH via flavin and iron-sulfur (2Fe-2S) redox centers to a terminal dioxygenase. The latter, which is also a multimeric enzyme consisting of either a homomers or a and β heteromers, catalyzes the incorporation of two hydroxyl groups on the aromatic ring at the expense of dioxygen and NAD(P)H. The consensus sequence of both the Rieske- and iron- binding motifs (SEQ ID NOS 6-7, respectively) as well as the spacing between them are precisely conserved in a hypothetical protein (translated from an ORF) from Synechocystis sp . PCC6803, which in addition, exhibits 66% amino acid identity to LLS1 among a stretch of more than 100 amino acids. Additionally, the Rieske center-binding site has also been detected in the partial sequence of two seemingly related ESTs (SEQ ID NOS 31-32, respectively) of unknown function, one each from rice and Arabidopsis. llsl and Cochliobolus carbonum Inoculation of llsl leaves with Cochliobolus carbonum Race " 1 causes a proliferation of llsl-type necrotic lesions in the middle to upper parts of the leaves. These llsl-type lesions superficially resemble C. carbonum lesions but they are sterile. That is, plating explants on carrot agar medium does not usually yield any C. carbonum fungal growth. Spontaneous llsl lesions occurring without inoculation are . also sterile and appear similar. Thus the lesions induced by C. carbonum inoculation are apparently llsl-type lesions and not susceptible C. carbonum lesions. This raises the question as to whether these lesions indicate that the llsl mutant is susceptible to C. carbonum or not. It seems likely that the llsl plants are resistant to C. carbonum, but that C. carbonum is able to trigger llsl lesion formation. The C. carbonum could be acting as a stress that sets off the llsl lesion development. After all, even abiotic stresses, such as needle pricking, will also induce llsl lesion formation. Inoculation of llsl leaves with Cochiobolus carbonum toxin plus or toxin minus causes few if any lesions to form in the middle to lower parts of the inoculated leaves. This observation is interpreted to mean that the llsl mutation possesses induced resistance to C. carbonum in that area of the leaf. While both spontaneous llsl lesions and C. carbonum lesions physically resemble each other, neither type was seen in this area of the leaf . In the middle transitional area there are some nascent smaller llsl lesions. It appears as though only the upper acropetal areas of the leaf at this stage of development, are capable of forming spontaneous llsl lesions or C. carbonum induced lesions . In the lower-middle areas of llsl leaves without any pathogen inoculation, a several fold elevation of PRl and chitinase proteins was observed on western blots over that of Llsl/llsl wildtype heterozygotes. Upon inoculation, the PRl and chitinase expression in this area of the leaves was elevated slightly in llsl and substantially in the Llsl/llsl heterozygotes, such that after inoculation both llsl and the wildtype heterozygotes have similar levels of PRl and chitinase. Thus it appears that: 1) elevated PR gene expression is correlated with resistance to C. carbonum in the lower middle area of the leaves, and 2) the PR gene induction exists prior to the resistance. llsl and Cochiobolus heterostrophus As was seen with C. carbonum, inoculation of llsl leaves with Cochiobolus heterostrophus also causes a proliferation of llsl-type necrotic lesions in the middle to upper parts of the leaves. These llsl-type lesions are generally distinguishable from C. heterostrophus necrotic lesions. These lls-type lesions are also sterile; that is, plating explants on carrot agar medium does not usually yield any C. heterostrophus fungal growth. Spontaneous llsl lesions occurring without inoculation are also sterile and appear similar. Thus the lesions induced by C. heterostrophus inoculation are apparently llsl-type lesions and not susceptible C. heterostrophus lesions. It appears that C. heterostrophus triggers formation of llsl lesions. C. heterostrophus appears to be acting as a stress that sets off the llsl lesion development. After all, even abiotic stresses, such as needle pricking, will also induced llsl lesion formation. Inoculation of llsl leaves with Cochliobolus heterostrophus causes few if any lesions to form in the middle to lower parts of the inoculated leaves. This observation was interpreted to mean that the llsl mutation possesses induced resistance to C. heterostrophus in that area of the leaf. Spontaneous llsl lesions and C. heterostrophus lesions are usually distinguishable by appearance, yet neither type was observed in this area of the leaf. In the middle transitional area there are some nascent smaller llsl lesions, so it appears as though only the upper acropetal areas of the leaf are capable of forming llsl lesions. However, the lack of C. heterostrophus lesions in this area of the leaf relative to their appearance in Llsl/llsl and Llsl/Llsl wildtype controls, indicates that llsl possesses resistance to C. heterostrophus in that area of the leaf. That the llsl heterozygotes are not resistant indicates that this resistance, like llsl lesion formation, is a recessive Mendelian trait. In the lower-middle areas of llsl leaves without any C. heterostrophus inoculation, a several fold elevation of PRl and chitinase proteins was observed on western blots over that of Llsl/llsl wildtype heterozygotes. Upon inoculation with C. heterostrophus , the PRl and chitinase in this area of the leaves is elevated slightly in llsl and substantially in the Llsl/llsl heterozygotes, such that after inoculation they have similar levels of PRl and chitinase. Thus it appears that elevated PR gene expression is correlated to resistance to C. heterostrophus in the lower middle area of the leaves, and that this elevated PR gene expression occurs prior to the inoculation and resistance. llsl and Puccinia sorghi (Rust ) Rust inoculation of llsl plants does not necessarily induce llsl-type necrotic lesions. It was observed that rust will infect llsl plants and produce sporulating lesions. This indicates that unlike C. carbonum, C. heterostrophus, and Puccinia sorghi , rust, a biotrophic pathogen, is able to infect llsl and Lls/llsl heterozygote control plants, The fact that P . sorghi will infect and form lesions indicates that P. sorghi can evade triggering llsl lesions formation and that it can survive and grow on llsl . The llsl mutation is therefore not necessarily rust resistant. Differences that may exist in rust susceptibility in the acropetal versus basipetal regions of the leaf have not been investigated. Western blots revealed that mutant llsl plants and Llsl/llsl wildtype heterozygote plants had similar levels of chitinase expression following rust inoculation. The expression of PRl, however, was slightly higher in the wildtype plants than in llsl mutants following rust inoculation. These experiments seem to indicate that although rust is able to avoid triggering llsl-type lesions formation in llsl , it still manages to trigger at least chitinase expression. These results may have important ramifications for understanding how pathogens are detected by the plant host, and if detected, whether by the same or different mechanisms, how the signaling pathways determine whether PR gene expression activated. To date no studies have isolated a protein (s) or gene(s) ubiquitously involved in the degradation of plant phenolics. Phenolics in plants are often sequestered in cell compartments until needed or synthesized only when required. Some phenolics however such as benzoic acid and salicylic acid have been proposed to play key roles in preconditioning cells to undergo cell death during the hypersensitive response as described by current models for systemic acquired resistance in dicot plants. One candidate that may fit well in this role is salicylic acid (SA) . SA, which exhibits a 10-50 fold increase during the HR and is also triggered in response to oxidative stresses associated with ozone or UN exposure (Hammond-Kosack and Jones (1996) Resistance Gene -dependent Plant Defense Responses, Plant Cell 8:1773-1791); Ryals et al . (1996) Systemic Acquired Resistance, Plant Cell 8:1809- 1819), is known to cause H 2 0 2 buildup (Chen et al . (1993) Involvement of Reactive Oxygen Species in the Induction of Systemic Acquired Resistance by Salicyclic Acid in Plants, Science 242:883-886) and transmute into a cell damaging free radical under oxidinzing conditions (Durner and Klessig (1996) Salicylic Acid is a Modula tor of Tobacco and Mammalian Catalases, J. Biol . Chem. , 271:28492-28501) . These attributes of SA indicate that it may be a mediator of cell death in llsl mutants, a hypothesis fuly compatible with the demonstrated dependence on SA of cell death associated with a number of Arabidopsis lsd mutants (Dangl et al . (1996) Dea th Don ' t Have no Mercy: Cell Death Programs in Plant -microbe Interactions , Plant Cell 8:1793- 1807; Weyman et al . (1996) Suppression and Restoration of Lesion Forma tion in Arabidopsis lsd mutants, Plant Cell 22:2013-2022) . However, as noted above, the possibility nevertheless remains that a novel compound or mechanism is responsible for lls -associated cell death. The predicted association of LLS1 with an iron-sulfur cluster suggests that it may also participate in oxidation- reduction reactions. Proteins that use iron-sulfur clusters as prosthetic groups often function as biosensors of oxidants and iron (Roualt and Klausner (1996) Iron-sulfur Clusters as Biosensors of Oxidants and Iron, Trends Biochem. Sci. 21:174-177) . LLS1 may also serve as a kind of rheostat such as that proposed for LSD1 in regulating cell death in plants (Jabs et al . (1996) Ini tiation of Runaway Cell Death in an Arabidopsis Mutant by Extracellular Superoxide, Science 273:1853-1856) . Working model for llsl function As noted earlier, the present invention is not dependent upon a particular mode of action. However, it is predicted that the LLS1 protein functions to inhibit the action of a cell "suicide factor" by degrading that factor. The suicide factor is a phenolic compound that is either a toxin or signal associated with photosynthetic stress or wounding or due to metabolic upset in the case of llsl/LeslOl double mutants. Phenolics can cause superoxide production formation by donating an electron to dioxygen while in a semiquinone form (Appel (1993) Phenolics in Ecological Interactions : The Importance of Oxidation, J ^ Chem. Ecol . 15:1521-1552). Photosynthetic organisms have evolved multiple mechanisms to dissipate excess energy and avoid the production of reactive oxygen intermediates (ROI) during photosynthesis. Free-radicals are scavenged by ascorbate, carotenoids, the xanthophyll cycle, alpha-tocopherol , glutathione, and various phenolics (Alscher et al . (1993), Antioxidants in Higher Plants) . The oxidative state of a cell influences dramatically the ability of phenolics to promote free radical formation (Appel (1993) Phenolics in Ecological Interactions : The Importance of Oxidation, J. Chem. Ecol . 19:1521-1552). The development of llsl lesions could result in cell death due to the inability to remove a toxic phenolic or signal that has accumulated in a cell . Whereas a toxin may directly inhibit basic metabolic processes a signal may trigger a programmed cell death pathway that is reminiscent of the hypersensitive response. Lesions thus spread because the release of the contents of dying cells cause oxidative stress in surrounding cells and result in the autocatalytic production of the cell suicide factor. Alternatively a signal for cell death may activate cell death programs in surrounding cells unless it is removed. The developmental gradient of llsl lesion expression may reflect the accumulation of a suicide factor in older cells. Young tissue does not form lesions when wounded and this may reflect the lack of accumulation of a suicide factor, the inability to yet synthesize that compound or the existence of a juvenile 12sl homolog. Protection of the plant tissue from light would directly reduce the concentration of the suicide factor and avoid lesion formation. The concentric circle appearance of llsl lesions may thus result from variation in the production of the suicide factor due to diurnal light cycles. Revertant sectors would be resistant to this suicide factor and the ability of lesions to "traverse" pale green or albino sectors in llsl/llsl io/io or llsl/llsl NCS7 double mutants would reflect the concentration and diffusibility of the toxic phenolics across tissues less able or unable to produce the suicide factor. In normal tissues functional LLSl limits the effect of a suicide factor released in the process of wounding or stress. Finally it is expected that if LLS1 affects phenolic metabolism that a change in phenolic profile would occur in 12sl plants. Significantly, this prediction is supported by the report that a para-coumaric ester accumulates in 22s2 lesioned plants but not in normal wild-type siblings or wild-type siblings inoculated with the fungus Cochliobolus heterostrophus (Obanni et al . (1994) Phenylpropanoid accumula tion and Symptom Expression in the Lethal Leaf Spot Mutant of Maize, Physiol. Mol. Plant Path. 44:379-388). llsl may play a role in the Hypersensitive Response A complex series of cellular events is envisaged to occur during the activation of defense responses in plants (Hammond-Kosact et al . (1996) Resistance Gene -dependent Plant Defense Responses, Plant Cell 8:1773-1791). Incompatible responses will often lead to the death of an infected cell within a few hours of infection. There is considerable evidence that this hypersensitive response (HR) is a form of programmed cell death activated by the plant cell. Lesion mimic mutations may cause an uncoupling of the regulatory steps of this process. Recent evidence has shown that control of cell death involves checkpoints that negatively and positively modulate the decision to progress to cell collapse. Evidence is provided by the observation that the lesion mimic phenotype of the 2sd2 and lsd6 mutations of Arabidopsis are suppressed in the presence of the transgene nahG which degrades salicylic acid (SA) . Application of 2,6 dichlorisonicotinic acid (a chemical inducer of systemic acquired resistance - SAR) restored lesion phenotype of these mutants (Dangl et al . (1996) Plant Cell 8:1793-1807). This result directly implicates SA in the signalling pathway that leads to cell death in these lesion mimics and that normally LSDl and LSD6 would serve to negatively modulate that pathway. acdl plants form spreading lesions in the presence of a functional 2sd2 gene suggesting that ACD1 operates downstream or on a separate pathway from LSDl. Also there is evidence to indicate that SA donates an electron to catalase and in so doing becomes a free radical which interacts with membrane lipids to promote lipid peroxides which further promote membrane damage and cell collapse. Collectively these results suggest that acdl functions downstream of lsdl to inhibit a cell death pathway that is promoted by superoxide via SA and it may be that acdl transcription is activated by LSDl. ACD1/LLS1 may degrade SA and thus negatively regulate a signalling pathway that could lead to runaway promotion of cell death. ACDl/LLSl may be positively regulated by competing sensors of well being within the cell via the LSDl protein and or other activators. Thus in an 12sl mutant what normally may constitute a minimal stress may become exaggerated through a runaway amplification loop and cell death pathways may be triggered resulting in lesion formation. This model predicts that nahG in an acdl/acdl mutant will abolish lesion formation. Cell death mechanisms in plants versus animals Lesion mimic genes are now providing insight into the kinds of genes involved in regulating cell death in plants. Three lesion mimic genes have now been cloned and do not have related counterparts in animal systems. This suggests that cell death is regulated in plants in a manner very different from models describing cell death regulation in animals although a role for ROI seems common to both systems. The recently cloned mlo locus from barley has been shown to encode a membrane protein and the lsdl gene from Arabidopsis may encode a transcriptional activator. Both of these genes may normally serve to interpret external or internal stress signals and when mutated turn on or off other genes that cause cell death or cell survival respectively. The 12s2 gene appears to be encode an enzyme involved in suppressing the spread of cell death through some aspect of phenolic metabolism. Phenolic production has long been long associated with cell death in plants but little understood at the molecular level. Studies of the cloned 22s2 gene may afford unexpected insights into this important aspect of plant physiology. Expression profile of lethal leaf spot 1 (llsl ) In leaves 2 and 4 of 16-days-olds wild-type seedlings (Mol7, B73) , the strongest expression of 22s2 is seen in both upper and lower epidermis and its derivatives (such as silica cells) , in sklerenchyma cells on either side of vascular bundles, and in protoxylem elements. A weaker, but clearly discernible expression signal is observed in bundle sheath, mesophyll cells and midrib parenchyma. Expression is undetectable in metaxylem, phloem and companion cells. In 7-day-old darkgrown wild-type seedlings (B73), 22sl expression can be detected at low levels in a uniform distribution throughout most leaf cells. Slightly elevated levels can be found in coleoptile and midrib of the two oldest leaves . In leaves of the dominant lesion mimic mutant Les 101, and in the 22s2 mutant itself, expression of 22s2 is essentially the same as in wild-type. For in situ expression analysis of llsl , a 0.7kb Notl- Pstl fragment from the middle of the cDNA was used to make labeled sense and antisense riboprobes . " Clones comprising the genomic sequence and cDNA sequence described herein were deposited on 14 November 1996 with the American Type Culture Collection, Rockville, Maryland, and given accession numbers ATCC 97791 and ATCC 97792. All publications and patent applications mentioned in the specification are indicative of the level of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it will be obvious that certain changes and modifications may be practiced within the scope of the appended claims. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Briggs , Steven P. Johal , Gurmukh S . Gray, John (ii) TITLE OF INVENTION: METHODS AND COMPOSITIONS FOR CONTROLLING CELL DEATH AND DISEASE RESISTANCE IN PLANTS (iii) NUMBER OF SEQUENCES: 65 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: BELL, SELTZER, PARK & GIBSON (B) STREET: P.O. Drawer 34009 (C) CITY: Charlotte (D) STATE: North Carolina (E) COUNTRY: USA (F) ZIP: 28234 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.30 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: US 08/810,009 (B) FILING DATE: 04-MAR-1997 (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Spruill, W. Murray (B) REGISTRATION NUMBER: 32,943 (C) REFERENCE/DOCKET NUMBER: 5718-4 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 919-881-3140 (B) TELEFAX: 919-881-3175 (C) TELEX: 575102 (2) INFORMATION FOR SEQ ID NO : 1 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1855 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 15..1574 (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 1 : CGTGCGGGGA GAAT ATG CGC GCG ACA ATC CCA GCC CTG TCG CTC CTG GTG 50 Met Arg Ala Thr lie Pro Ala Leu Ser Leu Leu Val 1 5 10 ACG CCG CGG CTC CCC TCG CTC GCC GTG CCG CTG GCT GGA GGC CGC CTC 98 Thr Pro Arg Leu Pro Ser Leu Ala Val Pro Leu Ala Gly Gly Arg Leu 15 20 25 CGC GAG GGC GGT CGT TCT CGG ACC CGC CTC CGC GTG GCG GCG CCG ACG 146 Arg Glu Gly Gly Arg Ser Arg Thr Arg Leu Arg Val Ala Ala Pro Thr 30 35 40 TCC GTA CCA GGG GAA GCG GCG GAG CAG GCG GAG CCG AGC ACG TCG GCG 194 Ser Val Pro Gly Glu Ala Ala Glu Gin Ala Glu Pro Ser Thr Ser Ala 45 50 55 60 CCC GAG TCC GGC GAG AAG TTC TCG TGG AGG GAT CAC TGG TAC CCG GTC 242 Pro Glu Ser Gly Glu Lys Phe Ser Trp Arg Asp His Trp Tyr Pro Val 65 70 75 TCC CTC GTC GAG GAC CTC GAC CCC AGC CGC CCC ACC CCG TTC CAG CTC 290 Ser Leu Val Glu Asp Leu Asp Pro Ser Arg Pro Thr Pro Phe Gin Leu 80 85 90 CTC AAC CGC GAC CTC GTC ATC TGG AAG GAA CCC AAG TCC GGC GAG TGG 338 Leu Asn Arg Asp Leu Val lie Trp Lys Glu Pro Lys Ser Gly Glu Trp 95 100 105 GTC GCG CTC GAC GAC CGC TGC CCC CAC CGC CTT GCC CCG CTC TCG GAG 386 Val Ala Leu Asp Asp Arg Cys Pro His Arg Leu Ala Pro Leu Ser Glu 110 115 120 GGC AGG ATC GAT GAG ACG GGG TGC TTG CAG TGC TCG TAT CAC GGA TGG 434 Gly Arg lie Asp Glu Thr Gly Cys Leu Gin Cys Ser Tyr His Gly Trp 125 130 135 140 TCA TTC GAT GGC TCC GGC GCC TGC ACC AAG ATC CCC CAG GCC ATG CCC 482 Ser Phe Asp Gly Ser Gly Ala Cys Thr Lys lie Pro Gin Ala Met Pro 145 150 155 GAG GGT CCT GAG GCC CGT GCG GTG CGG TCA CCG AAG GCG TGC GCG ATC 530 Glu Gly Pro Glu Ala Arg Ala Val Arg Ser Pro Lys Ala Cys Ala lie 160 165 170 AAG TTC CCC ACC CTC GTC TCC CAG GGG CTG CTC TTC GTG TGG CCC GAT 578 Lys Phe Pro Thr Leu Val Ser Gin Gly Leu Leu Phe Val Trp Pro Asp 175 180 185 GAG AAT GGG TGG GAG AAA GCG GCC GCC ACC AAG CCT CCA ATG TTG CCG 626 Glu Asn Gly Trp Glu Lys Ala Ala Ala Thr Lys Pro Pro Met Leu Pro 190 195 200 AAA GAA TTT GAG GAC CCG GCC TTC TCC ACG GTG ACA ATC CAG AGG GAC 674 Lys Glu Phe Glu Asp Pro Ala Phe Ser Thr Val Thr lie Gin Arg Asp 205 210 215 220 TTG TTC TAT GGT TAT GAT ACG TTG ATG GAG AAC GTC TCT GAT CCG TCC 722 Leu Phe Tyr Gly Tyr Asp Thr Leu Met Glu Asn Val Ser Asp Pro Ser 225 230 235 CAT ATA GAA TTT GCT CAC CAC AAG GTT ACT GGA CGA AGA GAT AGA GCC 770 His lie Glu Phe Ala His His Lys Val Thr Gly Arg Arg Asp Arg Ala 240 245 250 AGG CCT TTG ACA TTC AGG ATG GAA TCA AGT GGT GCC TGG GGT TAC TCA 818 Arg Pro Leu Thr Phe Arg Met Glu Ser Ser Gly Ala Trp Gly Tyr Ser 255 260 265 GGA GCA AAT TCT GGT AAT CCT CGC ATT ACT GCA ACT TTT GAG GCC CCT 866 Gly Ala Asn Ser Gly Asn Pro Arg lie Thr Ala Thr Phe Glu Ala Pro 270 275 280 TGT TAT GCA TTG AAC AAA ATA GAG ATA GAC ACA AAG TTA CCC ATT TTT 914 Cys Tyr Ala Leu Asn Lys lie Glu lie Asp Thr Lys Leu Pro lie Phe 285 290 295 300 GGC GAC CAG AAA TGG GTC ATA TGG ATT TGC TCT TTC AAC ATT CCA ATG 962 Gly Asp Gin Lys Trp Val lie Trp lie Cys Ser Phe Asn lie Pro Met 305 310 315 GCC CCA GGG AAG ACT CGT TCT ATT GTC TGT AGC GCT CGA AAC TTT TTC 1010 Ala Pro Gly Lys Thr Arg Ser lie Val Cys Ser Ala Arg Asn Phe Phe 320 325 330 CAG TTC ACA ATG CCA GGA AAA GCA TGG TGG CAG CTT GTT CCT CGA TGG 1058 Gin Phe Thr Met Pro Gly Lys Ala Trp Trp Gin Leu Val Pro Arg Trp 335 340 345 TAT GAA CAT TGG ACT TCA AAT TTG GTC TAT GAT GGC GAT ATG ATC GTT 1106 Tyr Glu His Trp Thr Ser Asn Leu Val Tyr Asp Gly Asp Met lie Val 350 355 360 CTT CAA GGC CAG GAG AAG ATT TTC CTA GCT GCA ACC AAG GAG TCT TCT 1154 Leu Gin Gly Gin Glu Lys lie Phe Leu Ala Ala Thr Lys Glu Ser Ser 365 370 375 380 ACG GAT ATT AAT CAG CAG TAC ACA AAG ATC ACA TTC ACG CCC ACA CAA 1202 Thr Asp lie Asn Gin Gin Tyr Thr Lys lie Thr Phe Thr Pro Thr Gin 385 390 395 GCT GAT CGA TTT GTT TTA GCA TTC CGC ACA TGG CTA AGG AAA TTT GGC 1250 Ala Asp Arg Phe Val Leu Ala Phe Arg Thr Trp Leu Arg Lys Phe Gly 400 405 410 AAT AGC CAG CCG GAG TGG TTT GGA AAT CCT ACA CAA GAA GCA TTG CCT 1298 Asn Ser Gin Pro Glu Trp Phe Gly Asn Pro Thr Gin Glu Ala Leu Pro 415 420 425 TCC ACC GTC CTT TCA AAG CGC GAG ATG CTA GAC AGA TAC GAG CAG CAC 1346 Ser Thr Val Leu Ser Lys Arg Glu Met Leu Asp Arg Tyr Glu Gin His 430 435 440 ACG TTG AAA TGC TCG TCC TGC AAA GGA GCA TAT AAT GCA TTC CAG AAT 1394 Thr Leu Lys Cys Ser Ser Cys Lys Gly Ala Tyr Asn Ala Phe Gin Asn 445 450 455 460 CTG CAG AAG GTA TTC ATG GGA GCG ACA GTA GTT TGC TGT GCT GCC GCT 1442 Leu Gin Lys Val Phe Met Gly Ala Thr Val Val Cys Cys Ala Ala Ala 465 470 475 GGT ATT CCT CCA GAT GTT CAG CTC AGG TTA TTG ATC GGT GCG GCT GCT 1490 Gly lie Pro Pro Asp Val Gin Leu Arg Leu Leu lie Gly Ala Ala Ala 480 485 490 TTG GTC AGT GCC GCT GTA GCA TAC GCA TTC CAT GAG CTC CAG AAG AAT 1538 Leu Val Ser Ala Ala Val Ala Tyr Ala Phe His Glu Leu Gin Lys Asn 495 500 505 TTT GTA TTC GTG GAT TAC GTG CAT GCT GAC ATT GAT TGAAAGATTC 1584 Phe Val Phe Val Asp Tyr Val His Ala Asp lie Asp 510 515 520 GTGAGGATCT GTTGTGCGAC ATCACTGGCT CGCGAGTCGT GTCTGTAGTC TAGGGCTCTA 1644 GGCGTCTAGC TAGGGAAAGT AACTTTTTGC CGGGTATAGG TCATATTGCT CACATATGTA 1704 TTTTGTATAG TGTATGCACT CAACTGTAGC CGATTCAGTG CGAAAATATA GTTTTTATGT 1764 TACTATCTAT TGGATTAAAA TTGTCTCCAG ATCCTTTTAG CATGTAAAAT GCCATTTTTC 1824 AAATGGAAGT TCTCAATTGC GCCCCTAGAC T 1855 (2) INFORMATION FOR SEQ ID NO : 2 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 520 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 2 : Met Arg Ala Thr lie Pro Ala Leu Ser Leu Leu Val Thr Pro Arg Leu 1 5 10 15 Pro Ser Leu Ala Val Pro Leu Ala Gly Gly Arg Leu Arg Glu Gly Gly 20 25 30 Arg Ser Arg Thr Arg Leu Arg Val Ala Ala Pro Thr Ser Val Pro Gly 35 40 45 Glu Ala Ala Glu Gin Ala Glu Pro Ser Thr Ser Ala Pro Glu Ser Gly 50 55 60 Glu Lys Phe Ser Trp Arg Asp His Trp Tyr Pro Val Ser Leu Val Glu 65 70 75 80 Asp Leu Asp Pro Ser Arg Pro Thr Pro Phe Gin Leu Leu Asn Arg Asp 85 90 95 Leu Val lie Trp Lys Glu Pro Lys Ser Gly Glu Trp Val Ala Leu Asp 100 105 110 Asp Arg Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg lie Asp 115 120 125 Glu Thr Gly Cys Leu Gin Cys Ser Tyr His Gly Trp Ser Phe Asp Gly 130 135 140 Ser Gly Ala Cys Thr Lys lie Pro Gin Ala Met Pro Glu Gly Pro Glu 145 150 155 160 Ala Arg Ala Val Arg Ser Pro Lys Ala Cys Ala lie Lys Phe Pro Thr 165 170 175 Leu Val Ser Gin Gly Leu Leu Phe Val Trp Pro Asp Glu Asn Gly Trp 180 185 190 Glu Lys Ala Ala Ala Thr Lys Pro Pro Met Leu Pro Lys Glu Phe Glu 195 200 205 Asp Pro Ala Phe Ser Thr Val Thr lie Gin Arg Asp Leu Phe Tyr Gly 210 215 220 Tyr Asp Thr Leu Met Glu Asn Val Ser Asp Pro Ser His lie Glu Phe 225 230 235 240 Ala His His Lys Val Thr Gly Arg Arg Asp Arg Ala Arg Pro Leu Thr 245 250 255 Phe Arg Met Glu Ser Ser Gly Ala Trp Gly Tyr Ser Gly Ala Asn Ser 260 265 270 Gly Asn Pro Arg lie Thr Ala Thr Phe Glu Ala Pro Cys Tyr Ala Leu 275 280 285 Asn Lys lie Glu lie Asp Thr Lys Leu Pro lie Phe Gly Asp Gin Lys 290 295 300 Trp Val lie Trp lie Cys Ser Phe Asn lie Pro Met Ala Pro Gly Lys 305 310 315 320 Thr Arg Ser lie Val Cys Ser Ala Arg Asn Phe Phe Gin Phe Thr Met 325 330 335 Pro Gly Lys Ala Trp Trp Gin Leu Val Pro Arg Trp Tyr Glu His Trp 340 345 350 Thr Ser Asn Leu Val Tyr Asp Gly Asp Met lie Val Leu Gin Gly Gin 355 360 365 Glu Lys lie Phe Leu Ala Ala Thr Lys Glu Ser Ser Thr Asp lie Asn 370 375 380 Gin Gin Tyr Thr Lys lie Thr Phe Thr Pro Thr Gin Ala Asp Arg Phe 385 390 395 400 Val Leu Ala Phe Arg Thr Trp Leu Arg Lys Phe Gly Asn Ser Gin Pro 405 410 415 Glu Trp Phe Gly Asn Pro Thr Gin Glu Ala Leu Pro Ser Thr Val Leu 420 425 430 Ser Lys Arg Glu Met Leu Asp Arg Tyr Glu Gin His Thr Leu Lys Cys 435 440 445 Ser Ser Cys Lys Gly Ala Tyr Asn Ala Phe Gin Asn Leu Gin Lys Val 450 455 460 Phe Met Gly Ala Thr Val Val Cys Cys Ala Ala Ala Gly lie Pro Pro 465 470 475 480 Asp Val Gin Leu Arg Leu Leu lie Gly Ala Ala Ala Leu Val Ser Ala 485 490 495 Ala Val Ala Tyr Ala Phe His Glu Leu Gin Lys Asn Phe Val Phe Val 500 505 510 Asp Tyr Val His Ala Asp lie Asp 515 520 (2) INFORMATION FOR SEQ ID NO : 3 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2822 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 3 : GCAACGCACA CAGACAGGCA GCGATGTCTT TCGCGGGTCA GTAAACCTCA CTCACACAGG 60 CTATTCGTCT TAAGTTTTTT TGTTCAACAT CACATACTTG TGTTGCTAAT GTAACAAAAA 120 AAATTCACAC GCCTCACAAA CATTACAATA TGATTCAAAA TAGACACTAA CCAAACCTTG 180 GAGGACTTTG TACTGGCTAG AGAACACCTA CTCTACTGCT ATGCTGCTTA CCCGAGACAG 240 AGGAAATACA CACGAGCAAC TGTTGTGGAC TTGTTGCAAA ATAGCAAGGA AAGGTATTAG 300 TAATAGCAAG CATAATTGTA GGAGCTGCAA GTATAACAAT GATAGTCTGC TCTTTAGTAC 360 CTTACATGTA TGAAATAAAA AACTATATAG GTAAAGTGAA CAACATGCGT TATGTAAATC 420 TAGCAGACTA TTGGATTGAA AAGAATTCAA TTACAAGGAC AAAGAATGAC TGACGAGGGC 480 AGCAACACAA TAACTAAATG TTCCAAAATG GTCAGATATG AAGGGCTCGA ACGCATGCAC 540 GGCATGATAT GCTAGTTGGG GCCGTTTCCG TCGGGCTTTA AAGATAAGGA AATCTGGATA 600 TGGACTAATG ATGTCTAATT TTTGTTAGAG CCTAGCGCCC TAGCATGCTA ACTAGAAGGT 660 TAATTTTGTT TCTATTTTTT GTTGCACCGA CTGAGCCAAC ATTCTTTTGT CTAGTAGTTT 720 ACATTTTAGT TACTACTCTC TTCGTCTAAA AAGTACTATA TCTCCATTTT TTAAAATGTC 780 TTGCTTTTTG AAGAGCACTA TCTTTTAAAA TCTTGACCAA CTATATAAAA GTACTTCTGA 840 TACATGATAG GTTTAATAAA ATATATAAAA TCTTATATTT TTAGTAAGTC TAGTCAAACT 900 TAAGAGCTTT TGATGTCGCA CATAGTTGTT TTAAACAAGG TGTTTGTTCA TGTTCGTTCT 960 AATATGTGGA TAGTATTCCG ATTCATTTCG CCAGAGGTGT GGCTGTGGAT ATTTGGTTAG 1020 AGCATCTTCA AGAAAACCCG TAAATCAACT CCAAAAACGT TTTGAGCCTC CCAACAGTCC 1080 CCCTTCCCCT CCCCATATTA CGCGTCAAGC ATTGTTCCCA ATCGTCCTCT GCGCATGCTG 1140 GTTCCCACGT GTATTTTCCT CGCGCGCAGT TCTGTTGGAG GAGGAAGGCG GGACGTTGGC 1200 ACTAGCGCTG GCTGGAGATT ATGGCCATCG CAATCAGTTT GTGGCAGTCA AATGCTTTGT 1260 TTTTTTGGCC GCTCATGTGA GTATCATTTC TGTGAAAACT ATCTAAATCA ATATGAATGT 1320 ATATTTCTTT AAGTCGTCAC GATAGGAAGA CTCCATCGTT CTAAAACCTA AACCATGCAC 1380 ACATATTCAT CTTTCTCCAA ACGCAAGTCT CGTGATATTT ATATTCTCGT GCCAGCTAGA 1440 TTATCTAGAA ATTTAGATTC TTAAAAAAAT TCTTTAGAAA AAAAATTATA CCAAACAGGA 1500 CCATGGTTTA AACTATTACG GATAAATAGC ATGACTACCT TAGTATTTAA ATGATATCAG 1560 TTGAAATATG TCGACTTATT TTATAGTTAG TATTATTAGA ACATGTTTAA ATAATTATCA 1620 CATTTAAACC AGATCTACAT ATAAACTATT TTGCTTGTCA ACTGCATCGC AAACTCACTT 1680 GCCTACCATC GGGATCGCGC TCGTATACAA GTGACACACT TTAAATGATT TAAGCCGCGA 1740 AAATTATAAA TGTACCATCC TCATTTGGCA AGTCTAAAGA TAGCTTTACC ATACAAATGA 1800 AACTAAATTT AAAATTCCAA GTAATAATTA GAAAAACTGA TTTGACAGTT TTTTCAGTAT 1860 ATATTTAGCA GCTCGCTAAA TCTGAATTTA GAAAGTTTTT TTGAAATGAG TTGAGATGCT 1920 CTTATAATGG TTACTATAGG TTGAGGGACG GAAGTAGTAG TAGAACTGGT AAACAAATTC 1980 GAATTTGATC TATTCAACTT TGTAGCTACT CAGCAAGATG CGAATTGCAA ACATCCGGCG 2040 GGGTGGATTC CGCCACGGCC CACGGGTGGG TTCGTGTCGT TCTCACCGCC GGTCAATCTC 2100 CCCTCCGCGC GGCGCAATTC GTCCCGGTGG GGACGGCTAG CTGGCCCAAT GCCAAAGCTC 2160 CACCGACAAA TGCCGCAAAG CGCCATGCGT GGTCGCGTAC AATTGCCTCC TTCCCCGCCC 2220 TTCCTCCCTT CCCTGCCGTG ACGCAACCAC ACTGCGCTCA CCATCGTGTA CAATGTATTC 2280 TCCCTAGCCG AACCGTATCA GTAGTTCTTA GGGGTGGGCG TTCGGGTTAC CCGAAATTTT 2340 CGGGTTGGGT AATTCAAGTT TTTTAAATTT CGGGTTTTGA GAATCAATAC CCGAAATTAC 2400 AACGGATTTT TCAATACCCG GAATTTCGGG TACCCGGAAT TTCGGGTTCG GGTTCGGGTA 2460 TTCCCAAACT ACCCGAACTA TTGTGTTGGC TTCATAAAAA CACATACACC CTATTAAATT 2520 AGTATAAAAA TATAGTTTGA ATAATGATAT ACATGGACAT ATAAAACACA AACAATCTAC 2580 AATCCCAAGT TATGCACACT TACACATAAT TATAGATGTA CAAACTTAAA TTATTAAAGC 2640 ATGACATGAG TACATGACAC ATGAAAGCCG GGTAATTCGG GTATTTCGGG TACCCGATTG 2700 TGATACCCGA ATTACCCGAA ATAATTTCGG GTTTTGCAAG TTGCTACCCG AAATTCCCAA 2760 ACAAAATTCG GGTTTCGGGT ATTTCGGGTT CGGGTTCGGG TATTCCAGGT TTGGGTTTCG 2820 GG 2822 (2) INFORMATION FOR SEQ ID NO : 4 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4015 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 4 : TTACGGGTTT TTTGCCCAGC CCTACTAGTT CTTCCCTCGC GTTCACTCCC CAGCGTGGGA 60 AAATCCCGGA ATTTTCTTGT TTGTCCACTG GTTTTCTTGC GCCAAAACCA GGTTTCTCCC 120 CGTTGCCGTG GCAGAACTCT GTTCTTGCCC AGTCTAGAAG ATCTGCACCG TTCCAACCAC 180 CGACTCCGGC CGCCAAGCAT ATAGCCAGCG CGGCGAAGAA TTCCCAACGC GAAAGCCAAA 240 ACCTCTTCAC TTCACTTCAC GTCGACACGT GCGGGGAGAA TATGCGCGCG ACAATCCCAG 300 CCCTGTCGCT CCTGGTGACG CCGCGGCTCC CCTCGCTCGC CGTGCCGCTG GCTGGAGGCC 360 GCCTCCGCGA GGGCGGTCGT TCTCGGACCC GCCTCCGCGT GGCGGCGCCG ACGTCCGTAC 420 CAGGGGAAGC GGCGGAGCAG GCGGAGCCGA GCACGTCGGC GCCCGAGTCC GGCGAGAAGT 480 TCTCGTGGAG GGATCACTGG TACCCGGTCT CCCTCGTCGA GGACCTCGAC CCCAGCCGCC 540 CCACCCCGTT CCAGCTCCTC AACCGCGACC TCGTCATCTG GAAGGAACCC AAGTCCGGCG 600 AGTGGGTCGC GCTCGACGAC CGCTGCCCCC ACCGCCTTGC CCCGCTCTCG GTACGGCGAC 660 CCGCATCCCT TCCTCGCCTC ATCCGTGTCC TACCGGATCT CTTCCTCGTT TCGGCTAATT 720 TTGGTCTGGG CATGTGCAGG AGGGCAGGAT CGATGAGACG GGGTGCTTGC AGTGCTCGTA 780 TCACGGATGG TCATTCGATG GCTCCGGCGC CTGCACCAAG ATCCCCCAGG CCATGCCCGA 840 GGGTCCTGAG GCCCGWGCGG TGCGGTCACC GAAGGCGTGC GCGATCAAGT TCCCCACCCT 900 CGTCTCCCAG GGGCTGCTCT TCGTGTGGCC CGATGAGAAT GGGTGGGAGA AAGCGGCCGC 960 CACCAAGCCT CCAATGTGCG TAGAGTCAGA CTTTGGACTG CGGCTAATTG GTTGGATTCA 1020 GTTTTGCATT TCGGTGTCTG AATTCGATCT TATTTGGTTT CAGGTTGCCG AAAGAATTTG 1080 AGGACCCGGC CTTCTCCACG GTGACAATCC AGAGGGACTT GTTCTATGGT TATGATACGT 1140 TGATGGAGAA CGTCTCTGAT CCGTCCCATA TAGAATTTGC TCACCACAAG GTACTTGGTA 1200 CAGTGAGAAA GCTTAGTTGC TTGCCACACT TAAGCACCAT GATAGTATTT TTCAGTTGAA 1260 AGTTGGTGAT TCGAGGAAAG ATGTTTTGTT GCAACCAATT TGTGTAGTTT GCTAAAAAAT 1320 CACCTCCTCA ATACTGTTTA ATTGTGTAGG CCTCTTATCG TTTCTGATTG CCAGTGTGCA 1380 AGTTTAACTA ACTGTTAGAT CTTAACTGTG GATGTACCCA TATATTTTTT TTGCATCATA 1440 GTTTTATTCT TTTTTACTTA TGCTGCATTG AAATTCCTCA GAAATGACTT ATAATGGGCA 1500 AAAGGGCTGA ATGGCTGAGT CTGGCCTCTT ATCGTTTCTA GATTGCCAGC GTGCAAGTTT 1560 AACTAAGGTC CCGTTTGGTT TGAGGGATTA AATATCAGTG CCTCCATTTT AGTCCCATTT 1620 AGTCCATAAA TTGACAAACG GTGGGACTAA AACAAGGACT AAACTGTTCT AGTCTCTAGT 1680 CCCTCAAGGG ATGACTCTAA GGGGCTAAAC CATAAAAATC CACTTTTTGG CCCTCCTTCA 1740 TTTCAGTTGC ACTAATGGCG GGAGGATGTT AAGGAGTATT TTGGTCTTCT TATGATTCAT 1800 TTAATGTGTT TTGAATACTT ATAGTTTTTA GAACCAAACA GGGAGGGACT AAATTTTAGT 1860 CTTCTAACTA AACTTTCGTC CCTGGACTAA AGGAACCAAA CCCTAACTGT TAGATCTTAA 1920 CTGTGGATGC ACCCATATAT ATTTTTGCAT CATAGTTTTA GTTCTTTTTT ACTTACGCTA 1980 CTTGCTTAGT CTGAACAGGC ATTAATAGGG TGTTTGGTTT GAGGGATTAG TTAGTTCACC 2040 CACTCATTCC TCTTTTCTTT GTTTGGTTTG TTGAATGGAG TAGGTTGGTC AGTGCATTAT 2100 CACATCATTC CTCAGACTAG TAGTTAGTAC TAGTATGAAG AATGGGGTCA TTCAACCAAA 2160 TTTAAGGAAT TGACTCATGA TGCATCACCA CATTTAGAAT GGAGTGGCTC CTCAAACCAA 2220 ACCCTATAAA TGACTGGCTG AGTTAATTGT GCTATCTGTG TGTCATGAAC TTGTGCCGGC 2280 AGCATAGACA AACAAAATGC TTTATTTTCT CGGGATACAT GGTTTCAGCA AATCCACTCA 2340 TGTTTCAGAT TTTAACTCTT CACAGGTTAC TGGACGAAGA GATAGAGCCA GGCCTTTGAC 2400 ATTCAGGATG GAATCAAGTG GTGCCTGGGG TTACTCAGGA GCAAATTCTG GTAATCCTCO 2460 CATTACTGCA ACTTTTGAGG CCCCTTGTTA TGCATTAAAC AAGTAAGTTT CAGAAAAGTA 2520 CCTGGTCATC TTTGAGTGTG GAGTGATTCT TATTTACCAC TTAAGCAATT CAGTCGTTAT 2580 ACGGTTCTGA ACTTCTGTTA ACTGGCTTGT ACAGAATAGA GATAGACACA AAGTTACCCA 2640 TTTTTGGCGA CCAGAAATGG GTCATATGGA TTTGCTCTTT CAACATTCCA ATGGCCCCAG 2700 GGAAGACTCG TTCTATTGTC TGTAGCGCTC GAAACTTTTT CCAGTTCACA ATGCCAGGAA 2760 AAGCATGGTG GCAGGTACAT GTGTGTTTAG TGTTTCCTTT ACTTAAGCTT TGTTTTCCTA 2820 TTTGTTTTGT CAACATAATC TTTTAACTGC TAAAACGAAC TTGTTCTCGC GTTTTTGTGG 2880 GAAACAAGGC AAAGGTCCCT AGTCCCTACT GTAGGCATAT ATTATTGGCA GAGTTTATTA 2940 CTTGGTCATG TTTGAATTTA TATGTGTACA GTCAAATGTT GATAGCTTCT TTCTCTTGGT 3000 GTAGCTTGTT CCTCGATGGT ATGAACATTG GACTTCAAAT TTGGTCTATG ATGGCGATAT 3060 GATCGTTCTT CAAGGCCAGG AGAAGATTTT CCTAGCTGCA ACCAAGGAGT CTTCTACGGA 3120 TATTAATCAG CAGTACACAA AGATCACATT CACGCCCACA CAAGCTGATC GATTTGTTTT 3180 AGCATGCCGC ACGTGGCTAA GGAAATTTGG CAATAGCCAG CCGGAGTGGT TTGGAAATCC 3240 TACACAAGAA GCATTGCCTT CCACCGTCCT TTCAAAGCGC GAGGTAAAAG CCATCTGGGT 3300 CACCAAAAAA GTTTCAGTAT AATATTTGCT TCAGACATAA AATATCTGAA TATGACAACC 3360 TTTTTGGTGG TCAAAGATCT GTTTTGCTTA CATTCTTAAT ACTCGATGCA TTGGTAAGTT 3420 ATTACAGTTA TCCTTTTTAC TCGATTTTTC CCTTTCTGAG CAGAACTATT ATCACGTCTT 3480 CATTGTTTGT ACACTTGGTT TCTATGACAC ACAAATTTTT ATTTTACATT ATCAGTTGTC 3540 ATATGAACTA ATGTATTTAC AGCAACCTGC TTAAGTGCTT AGTATCACAA AGGGACAAAT 3600 TCAATGAAAT ATTTGGAAAG ATAGTAGCGT CGAACCACTC TCACAGCTAG GCATTTGAGA 3660 ATAGTTACTT AACTGACAGC GAAGTTCACC TTCTACCGAC TGGATCTGGA AACAGTATCT 3720 TGAAGTAGTT CACACGTAAA CCTTCATCAG CTGTGTTTCT GGCTTCCAGT AACTCATGTA 3780 TTCTTATGAT TGACTTTGTG TTATGCAGAT GCTAGACAGA TACGAGCAGC TCTCGTTGAA 3840 ATGCTCGTCT TGCAAAGGAG CATATAATGC TTTCCAGAAT CTGCAGAAGG TATTCATGGG 3900 AGCGACAGTA GTTTGCTGTG CTGCCGCTGG TATTCCTCCA GATGTTCAGC TCAGGCTATT 3960 GATCGGTGCG GCTGCTTTGG TCAGTGCCGC TATAGCATAC GCATTCCATG AGCTC 4015 (2) INFORMATION FOR SEQ ID NO : 5 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 520 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (Xi) SEQUENCE DESCRIPTION: SEQ ID NO : 5 : Pro lie Gin Lys Asp Ser Leu Phe lie Ser His His Lys lie Pro lie 1 5 10 15 Lys Gly Leu Asn Phe Ser lie Lys lie Glu Thr Phe Pro Gin Pro Phe 20 25 30 Thr Arg Gly Gly Ala Ala Val Leu Tyr Pro Leu Arg lie Arg Arg Arg 35 40 45 Arg Ser Gly Ser Lys Lys Asn Thr Gly Gly Asp Lys Glu Glu Glu Gly 50 55 60 Ser Glu Phe Lys Trp Arg Asp His Trp Tyr Pro Val Ser Leu Val Glu 65 70 75 80 Asp Leu Val Pro Asn Val Pro Thr Pro Phe Gin Leu Leu Gly Arg Asp 85 90 95 Leu Val Leu Trp Phe Asp Arg Asn Asp Gin Lys Trp Ala Ala Leu Phe 100 105 110 Tyr Gly Tyr Asp Thr Leu Met Glu Asn Val Ser Asp Pro Ser His lie 115 120 125 Asp Phe Ala His His Lys Val Thr Gly Arg Arg Asp Arg Ala Lys Pro 130 135 140 Leu Pro Phe Lys Val Glu Ser Ser Gly Pro Trp Gly Phe Gin Gly Ala 145 150 155 160 Asn Asp Asp Ser Pro Arg lie Thr Ala Lys Val Ala Pro Cys Tyr Ser 165 170 175 Met Asn Lys lie Glu Leu Asp Ala Lys Leu Pro lie Val Gly Asn Gin 180 185 190 Lys Trp Val lie Trp lie Cys Ser Phe Asn lie Pro Met Ala Pro Gly 195 200 205 Lys Thr Arg Ser lie Val Cys Ser Ala Arg Asn Phe Asp Asp Leu Cys 210 215 220 Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg Leu Asp Glu Asn Gly 225 230 235 240 His Leu Gin Cys Ser Tyr His Gly Trp Ser Phe Gly Gly Cys Gly Ser 245 250 255 Cys Thr Arg lie Pro Gin Ala Ala Thr Ser Gly Pro Glu Ala Arg Ala 260 265 270 Val Lys Ser Pro Arg Ala Cys Ala lie Lys Phe Pro Thr Met Val Ser 275 280 285 Gin Gly Leu Leu Phe Val Trp Pro Asp Glu Asn Gly Trp Asp Arg Ala 290 295 300 Asn Ser lie Glu Pro Pro Arg Leu Pro Asp Asp Phe Asp Lys Pro Glu 305 310 315 320 Phe Ser Thr Val Thr lie Gin Arg Asp Phe Phe Gin Phe Ser Val Pro 325 330 335 Gly Pro Ala Trp Trp Gin Val Pro Arg Trp Tyr Glu His Trp Thr Ser 340 345 350 Asn Leu Val Tyr Asp Gly Asp Met lie Val Leu Gin Gly Gin Glu Lys 355 360 365 Val Phe Leu Ala Lys Ser Met Glu Ser Pro Asp Tyr Asp Val Asn Lys 370 375 380 Gin Tyr Thr Lys Leu Thr Phe Thr Pro Thr Gin Ala Asp Arg Phe Val 385 390 395 400 Leu Ala Phe Arg Asn Trp Leu Arg Arg His Gly Lys Ser Gin Pro Glu 405 410 415 Trp Phe Gly Ser Thr Pro Ser Asn Gin Pro Leu Pro Ser Thr Val Leu 420 425 430 Thr Lys Arg Gin Met Leu Asp Arg Phe Asp Gin His Thr Gin Val Cys 435 440 445 Ser Ser Cys Lys Gly Ala Tyr Asn Ser Phe Gin lie Leu Lys Lys Phe 450 455 460 Leu Val Gly Ala Thr Val Phe Trp Ala Ala Thr Ala Gly Val Pro Ser 465 470 475 480 Asp Val Gin lie Arg Leu Val Leu Ala Gly Leu Ser Leu lie Ser Ala 485 490 495 Ala Ser Ala Tyr Ala Leu His Glu Gin Glu Lys Asn Phe Val Phe Arg 500 505 510 Asp Tyr Val His Ser Glu lie Glu 515 520 (2) INFORMATION FOR SEQ ID NO : 6 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 6 Cys Xaa His Xaa Cys Xaa His 1 5 (2) INFORMATION FOR SEQ ID NO : 7 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 7 Glu Xaa Asp Xaa His Xaa His 1 5 (2) INFORMATION FOR SEQ ID NO : 8 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 8 : Asn Gin Cys His His Arg Gly Met Lys Leu Ser Arg Asp Asp Ala Gly 1 5 10 15 Asn Ala Lys Ala Pro Val Cys Thr Tyr His Gly Trp Ala His Asp lie 20 25 30 Ser Gly Gin 35 (2) INFORMATION FOR SEQ ID NO : 9 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 9 : Asn Gin Cys Arg His Arg Gly Met Arg lie Cys Arg Ser Asp Ala Gly 1 5 10 15 Asn Ala Lys Ala Pro Thr Cys Ser Tyr His Gly Trp Ala Tyr Asp lie 20 25 30 Ala Gly Lys 35 (2) INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: Asn Gin Cys Arg His Arg Gly Met Arg lie Cys Arg Ser Asp Ala Gly 1 5 10 15 Asn Ala Lys Ala Pro Thr Cys Thr Tyr His Gly Trp Ala Tyr Asp He 20 25 30 Ala Gly Asn 35 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: Asn Gin Cys Arg His Arg Gly Met Arg He Val Arg Ser Asp Gly Gly 1 5 10 15 Asn Ala Lys Ala Pro Thr Cys Thr Tyr His Gly Trp Ala Tyr Asp He 20 25 30 Ala Gly Asn 35 (2) INFORMATION FOR SEQ ID NO : 12 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 12 : Asn Gin Cys Arg His Arg Gly Met Arg He Cys Arg Ala Asp Ala Gly 1 5 10 15 Asn Ala Lys Ala Pro Thr Cys Ser Tyr His Gly Trp Ala Tyr Asp Thr 20 25 30 Ala Gly Asn 35 (2) INFORMATION FOR SEQ ID NO : 13 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 13 : Asn Gin Cys Arg His Arg Gly Met Arg He Cys Arg Ser Asp Ala Gly 1 5 10 15 Asn Ala Lys Ala Pro Thr Cys Ser Tyr His Gly Trp Ala Tyr Asp Thr 20 25 30 Ala Gly Asn 35 (2) INFORMATION FOR SEQ ID NO : 14 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 14 : Asn Gin Cys Arg His Arg Gly Met Arg He Cys Arg Ala Asp Gly Gly 1 5 10 15 Asn Ala Lys Ser Pro Thr Cys Ser Tyr His Gly Trp Ala Tyr Asp Ser 20 25 30 Ala Gly Asn 35 (2) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO : 15 : Asn Gin Cys Arg His Arg Gly Met Arg He Cys Arg Ala Asp Gly Gly 1 5 10 15 sn Ala Lys Ser Pro Thr Cys Ser Tyr His Gly Trp Ala Tyr Asp Thr 20 25 30 Gly Gly Asn 35 (2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16: Asn Ala Cys Ser His Arg Gly Ala Gin Leu Leu Gly His Lys Arg Gly 1 5 10 15 Asn Lys Thr Thr Tyr Thr Cys Pro Phe His Gly Trp Thr Phe Asn Asn 20 25 30 Ser Gly Lys 35 (2) INFORMATION FOR SEQ ID NO: 17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: Asn Ala Cys Ser His Arg Gly Ala Thr Leu Cys Arg Phe Arg Ser Gly 1 5 10 15 Asn Lys Ala Thr His Thr Cys Ser Phe His Gly Trp Thr Phe Ser Asn 20 25 30 Ser Gly Lys 35 (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18: Asn Ser Cys Arg His Arg Gly Ala Leu Leu Cys Pro Phe Ser Lys Gly 1 5 10 15 Asn Gin Lys Phe His Val Cys Arg Tyr His Gly Trp Ser Tyr Asp Ser 20 25 30 Ser Gly Arg 35 (2) INFORMATION FOR SEQ ID NO: 19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: Asn Val Cys Arg His Arg Gly Lys Thr Leu Val Ser Val Glu Ala Gly 1 5 10 15 Asn Ala Lys Gly Pro Val Cys Ser Tyr His Gly Trp Gly Phe Gly Ser 20 25 30 Asn Gly Lys 35 (2) INFORMATION FOR SEQ ID NO: 20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20: Asn Val Cys Arg His Arg Gly Lys Thr Leu Val Asn Ala Glu Ala Gly 1 5 10 15 Asn Ala Lys Gly Pro Val Cys Gly Tyr His Gly Trp Gly Phe Gly Ser 20 .25 30 Asn Gly Lys 35 (2) INFORMATION FOR SEQ ID NO: 21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21: Asn Val Cys Arg His Arg Gly Lys Thr He Val Asp Ala Glu Ala Gly 1 5 10 15 Asn Ala Lys Gly Pro Val Cys Gly Tyr His Gly Trp Gly Tyr Gly Ser 20 25 30 Asn Gly Lys 35 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: Ser Arg Cys Pro His Arg Gly Val Ser Leu Phe Met Gly Arg Val Lys 1 5 10 15 Lys Gly Gly Leu Arg Cys Val Tyr His Gly Trp Lys Phe Ser Ala Glu 20 25 30 Gly Lys (2) INFORMATION FOR SEQ ID NO: 23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23: Ser Arg Cys Pro His Arg Gly Val Ser Leu Phe Met Gly Arg Val Lys 1 5 10 15 Lys Gly Gly Leu Arg Cys Val Tyr His Gly Trp Lys Phe Ser Ala Glu 20 25 30 Gly Lys (2) INFORMATION FOR SEQ ID NO: 24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24: Lys Tyr Cys Pro His Arg Arg Val Ser Leu He Tyr Gly Arg Asn Lys 1 5 10 15 Asn Ser Gly Leu Arg Cys Leu Tyr His Gly Trp Lys Met Asp Val Asp 20 25 30 Gly Asn (2) INFORMATION FOR SEQ ID NO: 25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25: Pro Arg Cys Met His Arg Gly Thr Ser Leu Tyr Tyr Gly His Val Lys 1 5 10 15 Lys Ala Gly He Arg Cys Cys Tyr His Gly Trp Leu Phe Ala Val Asp 20 25 30 Gly Thr (2) INFORMATION FOR SEQ ID NO: 26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26: Asp Phe Cys Pro His Arg Gly Ala Pro Leu Ser Leu Gly Ser He Gin 1 5 10 15 Asp Gly Lys Leu Val Cys Gly Tyr His Gly Leu Val Met Asp Cys Asp 20 25 30 Gly Arg (2) INFORMATION FOR SEQ ID NO: 27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27: Gly Tyr Cys Arg His Met Gly Gly Asp Leu Ser Glu Gly Thr Val Lys 1 5 10 15 Gly Asp Glu Val Ala Cys Pro Phe His Asp Trp Arg Trp Gly Gly Asp 20 25 30 Gly Arg (2) INFORMATION FOR SEQ ID NO: 28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 28 : Asp Arg Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg He Asp 1 5 10 15 Glu Thr Gly Cys Leu Gin Cys Ser Tyr His Gly Trp Ser Phe Asp Gly 20 25 30 Ser Gly Ala 35 (2) INFORMATION FOR SEQ ID NO: 29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29: Asp Leu Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg Leu Asp 1 5 10 15 Glu Asn Gly His Leu Gin Cys Ser Tyr His Gly Trp Ser Phe Gly Gly 20 25 30 Cys Gly Ser 35 (2) INFORMATION FOR SEQ ID NO: 30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein ( i) SEQUENCE DESCRIPTION: SEQ ID NO: 30: Asp Gin Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg He Asn 1 5 10 15 Lys Ala Gly Gin Leu Glu Cys Pro Tyr His Gly Trp Thr Phe Ala Gly 20 25 30 Ser Gly Gin 35 ( 2 ) INFORMATION FOR SEQ ID NO : 31 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 34 amino acids (B ) TYPE : amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31: Ser Thr Cys Ala His Arg Ala Cys Pro Leu Asp Leu Gly Thr Val Asn 1 5 10 15 Glu Gly Arg He Gin Cys Pro Tyr His Gly Trp Glu Tyr Ser Thr Asp 20 25 30 Gly Asn (2) INFORMATION FOR SEQ ID NO: 32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32: Asn Thr Cys Ala His Arg Ala Cys Pro Leu His Leu Gly Ser Val Asn 1 5 10 15 Glu Gly Arg He Gin Cys Pro Tyr His Gly Trp Glu Tyr Ser Thr Asp 20 25 30 Gly Lys (2) INFORMATION FOR SEQ ID NO: 33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Ala Ala Thr Met Ser 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Ala Gly Thr Thr Thr 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Ala Gly Thr Met Ala 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Ala Gly Thr Met Ser 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Ala Gly Thr Thr Ser 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38 Ala Ala Glu Gin Phe Cys Ser Asp Ala Tyr His Ala Gly Thr Thr Ser 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Val Gly Thr Thr Ser 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 40: Ala Ala Glu Gin Phe Cys Ser Asp Met Tyr His Ala Gly Thr Thr Ser 1 5 10 15 His Leu (2) INFORMATION FOR SEQ ID NO: 41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41: Thr Ala Glu Asn Gly Ala Asp Gly Tyr His Val Ser Ala Val His Trp 1 5 10 15 (2) INFORMATION FOR SEQ ID NO:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 42: Gin Val Glu Asn Cys Ala Asp Gly Tyr His Val Ser Thr Val His Trp 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43: Gin Phe Glu Asn Gly Leu Asp Phe Tyr His Phe Gly Ser Thr His Ser 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 44: Pro Ala Glu Asn Phe Val Gly Asp Ala Tyr His Val Gly Trp Thr His 1 5 10 15 Ala (2) INFORMATION FOR SEQ ID NO: 45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 45: Pro Ala Glu Asn Phe Val Gly Asp Ala Tyr His Val Gly Trp Thr His 1 5 10 15 Ala (2) INFORMATION FOR SEQ ID NO: 46: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 46: Pro Ala Glu Asn Phe Val Gly Asp He Tyr His He Gly Trp Thr His 1 5 10 15 Ala (2) INFORMATION FOR SEQ ID NO: 47: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 47: Gin He Glu Asn Gly Ala Asp Gly Tyr His Val Gly Ser Val His Trp 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48: Asn Leu Glu Gly Lys He Asp Thr Ser His Phe Asn Pro Leu His Val 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 49: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein ( i) SEQUENCE DESCRIPTION: SEQ ID NO: 49: He Leu Glu Gly Ala He Asp Ser Ala His Ser Ser Ser Leu His Ser 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 50: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50: Asn Trp Glu Asn He Met Asp Pro Tyr His Val Tyr He Leu His Ser 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 51: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51: Met He Asp Asn Leu Met Asp Leu Thr His Glu Thr Tyr Val His Ala 1 5 10 15 (2) INFORMATION FOR SEQ ID NO : 52 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 52: He He Asp Asn Val Thr Asp Met Ala His Phe Phe Tyr He His Phe 1 5 10 15 (2) INFORMATION FOR SEQ ID NO: 53: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53: Leu Met Glu Asn Val Ser Asp Pro Ser His He Glu Phe Ala His His i 5 10 15 Lys (2) INFORMATION FOR SEQ ID NO: 54: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54: Leu Met Glu Asn Val Ser Asp Pro Ser His He Asp Phe Ala His His 1 5 10 15 Lys (2) INFORMATION FOR SEQ ID NO: 55: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 55: Leu Met Glu Asn Val Leu Asp Ser Ser His He Pro Tyr Thr His His 1 5 10 15 Lys (2) INFORMATION FOR SEQ ID NO: 56: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56: Asp Arg Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg He Asp 1 5 10 15 Glu Thr Gly Cys Leu Gin Cys Ser Tyr His Gly Trp Ser Pro Asp Gly 20 25 30 Ser Gly Ala 35 (2) INFORMATION FOR SEQ ID NO: 57: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 57: Asp Leu Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg Leu Asp 1 5 10 15 Glu Asn Gly His Leu Gin Cys Ser Tyr His Gly Trp Ser Pro Gly Gly 20 25 30 Cys Gly Ser 35 (2) INFORMATION FOR SEQ ID NO: 58: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 58: Asp Gin Cys Pro His Arg Leu Ala Pro Leu Ser Glu Gly Arg He Asn 1 5 10 15 Lys Ala Gly Gin Leu Lys Cys Pro Tyr His Gly Trp Thr Pro Ala Gly 20 25 30 Ser Gly Gin 35 (2) INFORMATION FOR SEQ ID NO: 59: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein ( i) SEQUENCE DESCRIPTION: SEQ ID NO: 59: Leu Met Glu Asn Val Ser Asp Pro Ser His He Glu Pro Ala His His 1 5 10 15 Arg (2) INFORMATION FOR SEQ ID NO: 60: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 60: Leu Met Glu Asn Val Ser Asp Pro Ser His He Asp Pro Ala His His 1 5 10 15 Arg (2) INFORMATION FOR SEQ ID NO: 61: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 61: Leu Met Glu Asn Val Leu Asp Ser Ser His He Pro Tyr Thr His His 1 5 10 15 Arg (2) INFORMATION FOR SEQ ID NO: 62: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 62: TGGGGAACTT GATCGCGCAC GCCTTCGG 28 (2) INFORMATION FOR SEQ ID NO: 63: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 63: TCGGGCATGG CCTGGGGGAT CTTGG 25 (2) INFORMATION FOR SEQ ID NO: 64: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "pimer" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 64: GGCCACGCGT CGACTAGTAC 20 (2) INFORMATION FOR SEQ ID NO: 65: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 35 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: other nucleic acid (A) DESCRIPTION: /desc = "primer" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 65: GTGCTCGGCT CCGCCTGCTC CGCCGCTTCC CCTGG 35

Claims: WHAT IS CLAIMED IS: 1. A substantially purified plant protein which is capable of suppressing cell death in plants. 2. The protein of claim 1, wherein said protein comprises a Rieske iron-coordinating motif. 3. The protein of claim 2, wherein said protein also comprises a mononuclear iron-binding site. 4. The protein of claim 1, wherein said protein has the amino acid sequence set forth in SEQ ID NOS 1 & 2. 5. The protein of claim 1, wherein said protein contains the carboxyterminal sequence set forth in SEQ ID NOS 1 & 2. 6. An isolated nucleotide sequence which encodes a plant protein which suppresses cell death in plants. 7. The nucleotide sequence of claim 6, wherein the plant protein comprises an amino acid sequence as set forth in SEQ ID NOS 1 & 2. 8. The nucleotide sequence of claim 6, wherein said plant protein has a carboxyterminal sequence as the carboxyterminal sequence set forth in SEQ ID NOS 1 & 2. 9. The nucleotide sequence of claim 6, wherein said sequence comprises the sequence set forth in SEQ ID NO: 1. 10. An isolated nucleotide molecule encoding a polypeptide capable of suppressing cell death in plants, said molecule having a sequence which hybridizes to the carboxyterminal region of the nucleotide sequence of claim 8 under stringent conditions. 11. An isolated nucleotide molecule encoding a polypeptide capable of suppressing cell death in plants, said molecule having a sequence which has at least 70% sequence similarity of the sequence of claim 9. 12. A transformed plant comprising a chimeric gene, said gene comprising a nucleotide sequence which encodes a protein which suppresses plant cell death operably linked with a promoter which is operable in the plant cell. 13. The transformed plant of claim 12, wherein said protein comprises a Rieske iron-coordinating motif. 14. The transformed plant of claim 13, wherein said protein is a plant protein. 15. The transformed plant of claim 14, wherein said protein comprises an amino acid sequence as set forth in SEQ ID NOS 1 & 2. 16. The transformed plant of claim 14, wherein said protein has a carboxyterminal sequence as the carboxyterminal sequence set forth in SEQ ID NOS 1 & 2. 17. The transformed plant of claim 14, wherein said nucleotide sequence comprises the sequence set forth in SEQ ID NOS 1 & 2. 18. Transformed seed from any of the plants of claims 12-17. 19. A method for controlling cell death in a plant, said method comprising transforming said plant with an expression cassette comprising a chimeric gene, said gene comprising a nucleotide sequence which encodes a protein which suppresses plant cell death operably linked with a promoter which is operable in the plant cell . 20. The transformed plant of claim 19, wherein said protein comprises a Rieske iron-coordinating motif. 21. The method of claim 20, wherein said protein is a plant protein. 22. The method of claim 20, wherein said protein comprises an amino acid sequence as set forth in SEQ ID NOS 1 & 2. 23. The method of claim 20, wherein said protein has a carboxyterminal sequence as the carboxy terminal sequence set forth in SEQ ID NOS 1 & 2. 24. The method of claim 21, wherein said nucleotide sequence comprises the sequence set forth in SEQ ID NO: 1. 25. A method for increasing resistance to disease in a plant, said method comprising transforming said plant with an expression cassette comprising a chimeric gene, said gene comprising a nucleotide sequence which encodes a protein which suppresses plant cell death operably linked with a promoter which is operable in the plant cell. 26. The method of claim 24, wherein said disease is a result of plant pathogens . 27. The method of claim 26, wherein said plant pathogens are selected from the group consisting of viruses, bacteria, insects, and fungi. 28. The method of claim 27, wherein said fungi is selected from the group consisting of Drechslera maydis, Fusarium moniliforme, Gibbe -el la zeae, and Cochliobolus heterostrophus . 29. The method of claim 25, wherein said protein has been modified to decrease protein activity. 30. The method of claim 29, wherein said protein has been modified by substitution of amino acids. 31. The method of claim 30, wherein said substitution comprises changing at least one Tyr residue to Ala. 32. The method of claim 25, wherein said protein comprises a Rieske iron-coordinating motif. 33. The method of claim 32, wherein said protein is a plant protein. 34. The method of claim 33, wherein said protein comprises an amino acid sequence as set forth in SEQ ID NOS 1 & 2. 35. The method of claim 33, wherein said protein has a carboxy terminal sequence as the carboxy terminal sequence set forth in SEQ ID NOS 1 & 2. 36. The method of claim 33, wherein said nucleotide sequence comprises the sequence set forth in SEQ ID NO: 1 37. An isolated nucleotide sequence comprising a promoter sequence which is capable of driving expression of a gene in a plant cell wherein said promoter natively drives the expression of a plant cell death suppressor protein. 38. The promoter of claim 37, wherein said promoter comprises the sequence set forth in SEQ ID NO: 3. 39. A chimeric gene comprising the promoter of claim 37, operably linked with a heterologous coding sequence. 40. A vector comprising the chimeric gene of claim 39. 41. A host cell comprising the vector of claim 40. 42. A plant which has been stably transformed with the chimeric gene of claim 40. 43. Transformed seed of the plant of claim 42. 44. A method for increasing transformation efficiency, said method comprising transforming a cell with a chimeric gene, said gene comprising a nucleotide sequence which encodes a protein which suppresses plant cell death operably linked with a promoter which is operable in the plant cell. 45. The method of claim 44, wherein said protein comprises a Rieske iron-coordinating motif. 46. The method of claim 45, wherein said protein is a plant protein. 47. The method of claim 46, wherein said protein comprises an amino acid sequence as set forth in SEQ ID NOS 1 & 2. 48. The method of claim 47, wherein said protein has a carboxyterminal sequence as the carboxyterminal sequence set forth in SEQ ID NOS 1 & 2. 49. The method of claim 47, wherein said nucleotide sequence comprises the sequence set forth in SEQ ID NO: 1. 50. An isolated nucleotide sequence which comprises a sequence encoding a plant protein which suppresses cell death in plants . 51. The nucleotide sequence of claim 50, wherein said sequence comprises the sequence set forth in SEQ ID NO: 4. 52. A probe for mapping the presence of a nucleotide sequence, wherein said probe comprises a portion of the nucelotide sequence of claim 51. 53. A chimeric gene comprising the nucleotide sequence of any of claims 6-11 operably linked with a heterologous promoter. 54. A method for producing male sterile plants, said method comprising: transforming a cell from a plant of interest with an expression cassette comprising a chimeric gene, said chimeric gene comprising a stamen promoter operably linked to a modified nucleotide sequence which encodes a protein which natively supresses cell death, and regenerating a transformed plant. 55. The method of claim 54, wherein said nucleotide sequence has been modified to encode a protein which exhibits a decrease in activity. 56. The method of claim 55, wherein said protein has been modified by substitution of amino acid residues. 57. The method of claim 56, wherein said substitution comprises changing at least one Tyr residue to Ala.

Inventors: BRIGGS STEVEN P, GRAY JOHN, GURMUKH JOHAL S, BRIGGS, STEVEN, P., GRAY, JOHN, GURMUKH, JOHAL, S.
Applicants: BRIGGS STEVEN P, GRAY JOHN, GURMUKH JOHAL S, PIONEER HI BRED INT, UNIV MISSOURI, BRIGGS, STEVEN, P., CURATORS OF THE UNIVERSITY OF MISSOURI, GRAY, JOHN, GURMUKH, JOHAL, S., PIONEER HI-BRED INTERNATIONAL, INC.
Application year: 1998
Main classification: C12N9
IPC codes: C12N9, C12N15