ACETYL-COA-CARBOXYLASE FROM CANDIDA ALBICANS

Abstract: The Acetyl-COA-carboxylase (ACCase) gene from Candida albicans.

Description: ACETYL-COA-CARBOXYLASE FROM CANDIDA ALBICANS The present invention relates to Acetyl-COA-carboxylase (ACCase) genes from Candida Albicans (C. albicans) .and methods for its expression. The invention .also relates to novel hybrid organisms for use in such expression methods. C. .albicans is .an important fung-al pathogen and the most prominent target organism for •antifung-al research. ACCase is an enzyme of fatty acid biosynthesis .and essential for fungal growth .and viability. Inhibitors of the ACCase enzyme should therefore be potent antifungals. The ACCase proteins in all organisms are homologous to each other but they .also differ significantly in the amino acid sequence. Because selectivity problems (for example fiingal versus hum.an) it is extremely important to optimise potential inhibitor leads directly against the target enzyme (C. albicans) and not against a homologous but non-identical model protein, for example from Saccharomyces cerevisiae (S. Cerevisiae). We have now successfully cloned the ACCase gene from C. albicans (hereinafter referred to as the C. Albicans ACC1 gene) and elucidated its full length DNA sequence .and corresponding polypeptide sequence, as set out in Figures 4 and 5 of this application respectively. The coding DNA sequence of the C. Albicans ACC1 gene is 6810 nucleotides in length and the corresponding protein sequence is 2270 amino acids in length. As will be explained below there are two forms of the C. Albicans ACC1 gene, the above numbers relate to the longer version, Metl . Therefore in a first aspect of the present invention we provide a polynucleotide encoding a C.albicans ACCase gene, in particular the (purified) C. albicans ACC1 gene as set out in Figure 5 hereinafter. It will be appreciated that the polynucleotide may comprise any of the degenerate codes for a particular amino acid including the use of rare codons. The polynucleotide is conveniently as set out in Figure 4. It will be apparent from Figure 4 that the gene is characterised by the start codons Metl and Met2 (as indicated by the first and second underlined atg codons, hereinafter referred to as atgl and atg2 respectively). Both forms of the gene starting from Metl and Met2 respectively are comprised in the present invention. The invention further comprises convenient fragments of any one of the above sequences. Convenient fragments may be defined by restriction endonuclease digests of sequence, suitable fragments include a full length C. Albicans ACCl gene (starting with Metl or Met2) flanked by unique StuI (5'-end)-NotI (3 '-end) restriction sites as detailed in Figure 6. We also provide a polynucleotide probe comprising any one of the above sequences or fragments together with a convenient label or marker, preferably a non-radioactive label or marker. Following procedures well known in the .art, the probe may be used to identify corresponding nucleic acid sequences. Such sequences may be comprised in libraries, such as cDNA libraries. We also provide RNA transcripts corresponding to any of the above C. Albicans ACCl sequences or fragments. In a further aspect of the invention we provide a C. albicans ACCl enzyme, especially the ACCl enzyme having the polypeptide sequence set out in Figure 5, in isolated and purified form. This is conveniently achieved by expression of the coding DNA sequence of the C. Albicans ACCl gene set out in Figure 4, using methods well known in the art (for example as described in the Maniatis cloning manual - Molecular Cloning: A Laboratory Manual, 2 nd Edition 1989, J. Sambrook, E.F. Fritsch & Maniatis). As indicated for Figure 4 above, the enzyme is characterised by two forms Metl and Met2. Both form of the enzyme are comprised in the present invention. The C. Albicans ACCl enzyme of the present invention is useful as a target in biochemical assays. However, to provide sufficient enzyme for a biochemical assay for C. Albicans ACCl (for example, for a high throughput screen for enzyme inhibitors) this has to be purified. Two major constraints impair this purification. 1) any new organism will necessitate deviation from published procedures because it will differ in its lysis .and protease activity. C. albicans is known to express and secrete many aspartyl proteges. 2) The expression of C. Albicans ACCl is very low and satisfying purification results can only be achieved if the enzyme is overexpressed. We have now been able to overcome these problems by controlled overexpression of the C. albicans ACCl in a Saccharomyces strain. This means that subsequent purification of the enzyme may then for example follow published procedures. Therefore in a further aspect of the present invention we provide a novel expression system for expression of a C. albicans ACCl gene which system comprises an S. cerevisiae host strain having a C. albicans ACCl gene inserted in place of the native ACCl gene from S. Cerevisiae, whereby the C. albicans ACCl gene is expressed. Preferred S. cerevisiae strains include.JK9-3Daα and its haploid segregants. The C. albicans ACCl gene is preferably over-expressed relative to that as may be achieved by a C. albicans wild type strain, ie under the control of its own ACCl promoter. Whilst we do not wish to be bound by theoretical considerations, we have achieved approximately 14 fold over-expression relative to the wild-type host S. cervisiae strain JK9-3D. This may be achieved by replacing the C. albicans promoter in the expression construct by a stronger .and preferably inducible promoter such as the S. cerevisiae GAL1 promoter. Controlled overexpression is used to improve expression of a C. albicans polypeptide relative to expression under the control of a C. albicans promoter. In addition using procedures outlined in the accompanying examples we have been able to isolate a fully functional C. albicans ACCl gene as determined by 100% inhibition by SoraphenA. The novel expression system is conveniently prepared by transformation of a heterozygous ACCl deletion strain of a convenient S. cerevisiae host by a convenient plasmid comprising the C. albicans ACCl gene. Transformation is conveniently effected using methods well known in the art of molecular biology (Ito et al. 1983). The plasmid comprising the C. albicans ACCl gene and used to transform a convenient S. cerevisiae host represents a further aspect of the invention. Preferred plasmids for insertion of the C. Albicans ACCl gene include YEρ24, pRS316 and pYES2(Invitrogen). The heterozygous ACCl deletion strain of a convenient (diploid) S. cerevisiae host is conveniently achieved by disruption preferably using an antibiotic resistance cassette such as the kanamycin resistance cassette described by Wach et al (Yeast, 1994, K), 1793-1808). The expression systems of the invention may be used together with, for example cell growth and enzyme isolation procedures identical to or analogous to those described herein, to provide an acetyl-COA-carboxylase (ACCase) gene from C.albicans in sufficient quantity and with sufficient activity for compound screening purposes. In a further aspect of the invention we provide the use of an acetyl-COA-carboxylase (ACCase) gene from C.albicans in assays to identify inhibitors of the polypeptide. In p.articul.ar we provide the their use in pharmaceutical or agrochemical research. As presented above the C. albicans ACCl enzyme may be used in biochemical assays to identify agents which modulate the activity of the enzyme. The design and implementation of such assays will be evident to the biochemist of ordinary skill. The enzyme may be used to turn over a convenient substrate whilst incorporating/losing a labelled component to define a test system. Test compounds are then introduced into the test system and measurements made to determine their effect on enzyme activity. Particular assays are those used to identify inhibitors of the enzyme useful as antifungal agents. By way of non-limiting example, the activity of the ACCl enzyme may be determined by (i) following the incorporation (HCO 3 , Acetyl-CoA) or loss (ATP) of a convenient label from the relevant substrate (T.Tanabe et al, Methods in Enzymology, 1981, 71, 5-60; M. Matasuhashi, Methods in Enzymology, 1969, 14, 3-16), (ii) following the release of inorganic phosphate from ATP (P. Lanzetta et al, Anal. Biochem. 1979, 100, 95-97), or (iii) following the oxidation of NADH in a coupled assay, for example using either fatty acid synthetase or pyruvate kinase/lactate dehydrogenase enzymes. Convenient labels include carbonl4, tritium, phosphorous32 or 33. Any convenient test compound(s) or libr-ary of test compounds may be used. Particular test compounds include low molecular weight chemical compounds (molecul-ar weight less than 1500 daltons) suitable as pharmaceutical agents for human, animal or plant use. The enzyme of the invention, and convenient fragments thereof may be used to raise antibodies. Such antibodies have a number of uses which will be evident to the molecular biologist of ordinary skill. Such uses include (i) monitoring enzyme expression, (ii) the development of assays to measure enzyme activity and precipitation of the enzyme. In addition we provide antisense polynucleotides specific for all or a part of an ACC 1 polynucleotide of the invention. The invention will now be illustrated but not limited by reference to the following Table, Example, References and Figures wherein: Table 1 shows the comparative properties of native and recombinant acetyl-CoA carboxylase enzymes Figure 1 shows partial sequence from the C. albicans genome. Underlined regions were used to derive PCR primers, to generate a C. albicans ACC 1 specific probe. Figure 2 shows cloned fragments of the C. albicans ACCl gene isolated from genomic DNA libraries. Arrows indicate extension of the fragment beyond the region displayed. Figure 3 shows sequenced Xbal-HinDIII and HinDIII subclones of clone CLSl-bl . Figure 4 shows the full DNA sequence of the C. albicans ACCl gene. The atg start codons for Metl and Met 2 are in lower case and underlined, as is the tag stop codon. Figure 5 shows the full protein sequence of the C. albicans ACCl gene. Putative start codons for Metl and Met2 .are shown in bold. Figure 6 shows the generation of a tailored ACCl gene (minus promoter) for expression under control of the GALl promoter in plasmid pYES2. From the initial ACCase gene (linel) the core SacI-BamHI (line3) is modified by the addition of 3' BamHI-NotI (line2) .and 5' StuI-SacI (different fragments for Metl and -2 lines 5 and 7 respectively) to generate the final "portable" gene flanked by StuI-NotI (lines 6 and 8). Figure 7 shows the results of the in-vitro ACCase enzyme assay set out in the accompanying Example when Soraphen A (a specific inhibitor of the ACCase enzyme) was supplied (X-axis) over the range 0.1 nM- 1 OOμM in the dose response regimen of the assay. Example 1 Cloning of the C. albicans ACCl gene and generation of a heterologous S. cerevisiae expression system: 1) Probe generation We used the polymerase chain reaction (PCR) to generate a DNA probe between and including the underlined regions in Figure 1 2) Identification of clones from a C. albicans genomic library hybridising to the ACCase probe The PCR product was labelled using an "ECL direct nucleic acid labelling and detection kit" (Amersham) as described by the supplier. The PCR product (probe) was then shown to hybridise to S. cerevisiae (weakly) and C. albicans genomic DNA. in a Southern blot procedure (as described Maniatis, 1989). Two genomic DNA libraries (CLS1 and CLS2) of C. albicans (in the yeast-E. coli shuttle plasmids YEp24 and pRS316 respectively, (as described in. Sherlock et al. 1994, source: Prof. John Rosamond, Manchester University) were used to isolate fragments hybridising with the probe which was radiolabelled using "Ready To Go" dCTP labelling beads (Pharmacia, as described by the manufacturer). The colony hybridisation was carried out as described by Maniatis (1989). Hybridising colonies were identified, plasmid DNA isolated, purified (Quiagen maxiprep, as described by the supplier) and sequenced (Applied Biosystems, model 377 sqeuencer) from their junctions with the plasmid. Several fragments carrying partial ACCase gene sequence as well as one full length clone could be identified (Figure 2). 3) Sequencing of the cloned gene, comparison with ACCases from S. cerevisiae, other fungi and higher eukaryotes (plants, mammals, man) The bulk of the sequence of the C. albicans ACCl gene was determined (on both strands) using flanking sequence- or insert sequence-specific primers from defined HinDIII and Xbal-HinDIII subfragments (of clone CLSl-bl) cloned into pUC19 (see Figure 4). The promoter and 5' coding region absent from this clone was established from CLS2-dl and the gene's 3' end from CLS2-13 using insert specific primers. All junctions including the ones between the HinDIII subfragments were verified from the full length clone CLS2-13 (in Yep24. The full length DNA sequence of C. albicans (Ca) ACCl is shown in Figure 5a .and the protein translation in Figure 5b. The two potential start Methionines, Metl and Met2 are shown in bold The protein is homologous to ACCases of other fungi (S. cerevisiae, S. pombe and U maydis) and also to the plant (Brassica napus), mammalian (sheep, chicken and rat) and human enzymes. Of the two potential start codons of C. albicans ACCl, Met 2 seems the more likely one as the sequence between Metl and Met2 is unrelated to the other ACCases and indeed to any other protein sequence in the EMBL/Genbank database. The high degree of homology between ACCases of different species .and the apparent lack of an identifiable fungal subgroup makes it even more important to use the actual target enzyme (here from the pathogen C. albicans) as a screening tool to identify specific inhibitors. 4) Generation of a heterozygous ACCl deletion strain of S. cerevisiae As ACCase is an essential enzyme, only one allele of a diploid cell can be deleted without loss of survival. One ACCl gene of a diploid S. cerevisiae strain (JK9-3Daa, Kunz et al. 1993) was therefore disrupted using the kanamycin resistance cassette as described by Wach et al. using the protocol described therein. Sporulation of the heterozygous diploid (ACCl/accl::KANMx) yields only two viable spores (which are kanamycin-sensitive) showing the essentiality of the ACCl gene as well as the characteristic arrest phenotype for the two inviable spores (as published by HaBlacher et al, 1993). 5) Complementation of a S. cerevisiae ACCl deletion with the cloned Candida gene, Ca ACCl The heterozygous ACCl/accl ::KANMx strain was transformed with one full length C. albicans gene (CLS2-13 in Yep24). Expression of the gene from this plasmid will be due to functionality of the Candida ACCl promoter in the heterologous S. cerevisiae system. Complementation of the knockout was demonstrated by sporulating the diploid tr.ansform.ants. In most cases 3-4 viable (haploid) spores were detected. The analysis of tetrads indicated that kanamycin-resistant colonies were only formed if they also contained the complementing CLS2-13 plasmid, as indicated by the presence of the URA3 tr-ansformation marker. This clearly shows that the C. albicans gene fully complements the ACCase function in S. cerevisiae. Therefore the strain generated can be used to screen for inhibitors which are specific for the Candida enzyme in the absence of a background of Saccharomyces enzyme. As demonstrated by its functionality, the heterologous protein folds correctly in the host, S. cerevisiae, where it must also have been correctly biotinylated by the S. cerevisiae machinery (carried out by ACC2, encoding protein-biotin-ligase). To facilititate purification of C. albicans ACCase, it is beneficial to achieve overexpression of the protein in a suitable host. Therefore the C. albicans promoter was replaced by the stronger and inducible S. cerevisiae GALl promoter. As the Candida sequence had revealed two potential start codons (see Figure 4) for the ACCl reading frame, both versions were placed under GALl control. To generate appropriate restriction sites for cloning, the ACCl gene was modified via PCR at both ends (see Figure 6 above), and cloned into plasmid pYES2 (Invitrogen) as a StuI-NotI fragment into HinDIII (fill-in)-Notl sites of the vector. The identity of the PCR-modified gene-p-arts with the original ones was confirmed by sequencing. Both constructs (Metl and Met2) complement the S. cerevisiae ACCl knockout when the cells .are grown on galactose but not on glucose (where the GALl promoter is switched off). Growth is very poor if the gene is transcribed initiating at Metl, whereas Met2 restores wild type growth rates in S.cerevisiae. 6) Overexpression of the Ca ACCase to facilitate protein purification and use for screening purposes Materials Growth Media :- Sabouraud Dextrose broth Yeast peptone dextrose broth (YPD) Yeast peptone galactose broth (YPGal) (i.e. 2% w/v galactose) Growth of cells Candida albicans B2630 (Janssen Pharmaceutica, Beerse, Belgium) was maintained on Sabouraud dextrose agar slopes at 37 ^C which were subcultured biweekly. For the growth of liquid cultures for experiments, C. albicans grown on Sabourauds dextrose agar for 48 h at 37°C was used to inoculate 50 ml Sabouraud dextrose broth containing 500μg/l d- biotin. This was incubated for 16 h at 37 ^C on a platform shaker (150 rpm). 1.5 ml of this culture was added to each of 24 x2 litre conical flasks, each containing 1 litre of Sabouraud dextrose broth containing 500μg/l d-biotin, giving a final inoculum concentration of approximately 1.5x10^ cfu ml "1 . The cultures were grown for 9 h, at 37 ^C (log phase) with shaking (150 rpm). Cell numbers in liquid culture were determined spectrophotometrically (Philips PU8630 UV/VIS/NIR Spectrophotometer) at 540 nm in a 1 cm path length cuvette. Absorbance was linearly related to cell number up to an OD. of 2.0. Saccharomyces cerevisiae strains Meyl34 and CLS2-13 were maintained on Yeast peptone dextrose (YPD) agar plates at 30 "f , which were subcultured biweekly. For the growth of liquid cultures for experiments, the S. cerevisiae strains were grown on YPD agar for 48 h at 30 °C and were then used to inoculate 50 ml YPD broth containing 500μg/l d- biotin, which was incubated at 30^C for 16h on a platform shaker (200 rpm). 2.0 ml of this culture (approx. 4 x 10 8 cfu/ml) was added to each of 24 x 2 litre conical flasks, each containing 1 litre of YPD broth containing 500μg/l d-biotin, giving a final inoculum concentration of approximately 8 xlO^ cfu/ml. The cultures were grown for 9 h, at 30 ^C (log phase) with shaking (200 φm). Cell numbers in liquid culture were determined spectrophotometrically (Philips PU8630 UV/VIS/NIR Spectrophotometer) at 540 nm in a 1 cm path length cuvette. Saccharomyces cerevisiae strains PNS 117a 5C, PNS 117b 6A, and PNS 120a 6C were maintained on Yeast peptone galactose (YPGal) agar plates at 30 ^C which were subcultured biweekly. For the growth of liquid cultures for experiments, the S. cerevisiae strains were grown on YPGal agar for 48 h at 30 °C and were then used to inoculate 50 ml YPGal broth containing 500μg/l d-biotin and 200μg/ml kanomycin, which were incubated at 30^C for 30h on a platform shaker (200 φm). 2.0 ml of this culture (approx. 4 x 10* cfu/ml) was added to each of 24 x2 litre conical flasks, each containing 1 litre of YPGal broth containing 500μg/l d-biotin .and 200μg/ml kanomycin, giving a final inoculum concentration of approximately 8 xlO^ cfu/ml. The cultures were grown for approximately 23h at 30 ®C (log phase) with shaking (200 φm). Determination of cell number Cell numbers were determined using a standard viable count agar based plating method, using the appropriate agar media. Preparation of fungal ACCase enzyme Cultures of the appropriate yeast strains were grown to the exponential phase of growth (for Saccharomyces and Candida strains respectively). These were then harvested by centrifugation (4400 g, lOmin, 4 °C), washed twice in 700ml of 50mM Tris pH7.5 containing 20% w/v gylcerol, resuspending the cell pellet each time. The final washed pellet was fully resuspended into a thick slurry using 10 to 20ml of buffer (50mM Tris pH7.5 containing ImM EGTA, ImM EDTA (disodium salt), ImM DTT, 0.25mM Pefabloc hydrochloride, 1 μM Leupeptin hemisulphate, 1 μM Pepstatin A, 0.5μM Trypsin inhibitor .and 20% w/v glycerol). The volume of buffer required w-as dependent on the total packed cell wet weight, (i.e. 1ml buffer added per 6gm of packed wet cell pellet). The cell paste was homogenised using a pre-cooled Bead-Beater (Biospec Products,Bartlesville, OK 74005) with 4 x 10 second Bursts, allowing 20 second intervals on ice. The preparation was then centrifuged at 31,180g for 30 minutes. After centrifugation the supernatant was immediately decanted into a container, then aliquoted before snap freezing in liquid nitrogen. The preparation was then stored at -80°C and was found to be stable for at least 2 months. All enzyme preparation steps were carried out at +4°C, unless otherwise stated. In-vitro ACCase enzyme assay The assay was conducted in 96 well, flat bottomed polystyrene microtitre plates. All test and control samples were tested in duplicate in this assay. lOOμl of the ACCase enzyme preparation (in 50mM Tris pH7.5 containing ImM EGTA, ImM EDTA (disodium salt), ImM DTT, 0.25mM Pefabloc hydrochloride, lμM Leupeptin hemisulphate, lμM Pepstatin A, 0.5μM Trypsin inhibitor, and 20% w/v glycerol) was added to each well of the microtitre plate. Each well contained either a 3μl test sample made up in DMSO or 3μl DMSO alone (NB. Final DMSO concentrations in the assay were 1.48% v/v). The microtitre plates were placed in a water bath maintained at 37°C. 1 Oμl of [ 14 C] NaHCO 3 containing 9.25kBq in 378mM NaHCO 3 was then added to each well. The reaction was initiated by the addition of lOOμl of Acetyl Coenzyme A containing assay buffer (50mM Tris pH7.5 containing 4.4 ImM ATP(disodium salt), 2. ImM Acetyl Coenzyme A, 2.52mM DTT, 10.5mM MgCl 2 , and 0.21% w/v Albumin [Bovine, fraction V]), (removed from ice 5 minutes before use) to each well. The tubes were incubated at 37°C for 5 minutes. The reaction was then terminated by the addition of 50μl of 6M HC1 to each well. In parallel, a pre-stopped assay control was set up which involved adding the 50μl of 6M HC1 prior to [ 14 C] NaHCO 3 and the assay buffer (No further HC1 additions were made to these wells after the 5 minute incubation). The DPM values for the pre-stopped assay were subtracted from the normal assay situation. After the addition of the stop reagent the plates were left open in the water bath for a further 30 minutes to allow the 14 CO 2 to escape. After this time 150μl of each reaction mixture were applied onto individual GF/C glass microfϊbre filter discs and allowed to dry thoroughly before adding scintillation fluid. Radioactivity in the samples was then determined by scintillation counting (Wallac WinSpectral 1414, Turku, Finland). IC50's were calculated from the data using non-linear regression techniques available in the ORIGIN software package (Microcal Software Inc., Massachusetts, USA). Soraphen A which is a specific inhibitor of ACCase was supplied over the range 0.1 nM- 1 OOμM in the dose response regimen of the assay. Protein determination The total protein concentration of each ACCase preparation used was determined by the Coomasie Blue method (Pierce, Illinois, USA), (using 1cm path length cuvettes read 595nm (Philips PU8630 UV/VIS/NIR Spectrophotometer). In-vitro antifungal activity Compounds were tested over a concentration range of 1024 - 0.00098 μg/ml by a broth-dilution method in microtitre plates using doubling dilutions in YPD or YPGal (both containing 500μg/l d-biotin). Stock solutions of inhibitors were prepared at 51.2mg/ml in Dimethyl sulphoxide (DMSO) (final assay concentration of DMSO was 2% v/v). Each Yeast culture was added to the well to give a final 10 4 cfu/well. The plates were incubated at 30°C for 48h and MIC's determined visually. Discussion Expression of ACCase, a biotinylated protein, was monitored by a "biotin-avidin affinity western blot" as described by Haβlacher et al., 1993. Expression of the C. albicans ACCl gene from its own promoter from plasmid Yep24 was comparable to that of the S. cerevisiae gene (no overexpression). Expression under control of the GALl promoter however, was considerably higher indicating a drastically increased level of biotinylated and therefore fully functional enzyme. Transcription of the gene was fully induced as the cells had to be grown on galactose to be viable. On glucose the GALl promoter is completely off, causing the cells to arrest and eventually die due to insufficient supply of ACCase). The S. cerevisiae strain described in this application is a convenient source of the C. albicans enzyme. The engineered strain possesses no residual background ACCase because the gene coding for the S. cerevisiae enzyme had been removed. Congenic versions of such a strain (genetically identical apart from the ACCase gene carried) expressing different ACCases (e.g. the different human (Abu-Elheiga et al. 1995), mammalian (Lopez-Casillas et al., 1988, Takai et al. 1988, Barber et al., 1995)), plant (Schulte et al., 1994) or other fungal enzymes (Al-Feel et al., 1992, Saito et al., 1996, Bailey et al, 1995) ) can be used as tools for screening. Differences in growth of such strains may be solely dependent on differences in their ACCase activity. Differential growth in the presence of ACCase inhibitors (for example soraphenA or compounds yet to be identified) indicates selectivity of the drug towards one type of the ACCase enzyme. References: Abu-Elheiga L., Jayakum.ar A., Baldini A., Chirala S.S., Wakil S.J.; Proc. Natl. Acad. Sci. U.S.A. 92: 4011-4015(1995). Al-Feel W., Chirala S.S., Wakil S.J.; Proc. Natl. Acad. Sci. U.S.A. 89:4534-4538(1992). Bailey A.M., Keon J.P.R., Owen J., Hargreaves J.A.; Mol. Gen. Genet. 249:191-201(1995). Barber M.C., Travers M.T.; Gene 154:271-275(1995). Hafilacher M., Ivessa A. S., Paltauf F., Kohlwein S. D.; J. Biol. Chem. 268:10946-10952 (1988). Ito, H., Fukuda, Y., Murata, K., Kimura, A.; J. Bacteriol. 153: 163-168 (1983) Kunz. J., Henriquez, R., Schneider, U., Deuter-Reinh.ard, M., Mowa, N.R., Hall, M.N.; Cell 73: 585-596 (1993) Lopez-Casillas F., Bai D.-H., Luo X., Kong I.-S., Hermodson M.A., Kim K.-H.; Proc. Natl. Acad. Sci. U.S.A. 85:5784-5788(1988). Maniatis T., Frisch E. F., Sambrook J.; Molecular Cloning, Cold Spring Harbour Laboratory Press (1989) Saiki R. K., Gelfand D. H., Stoffel S., Sharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A.; Science 239: 487-494 (1988) Saito A., Kazuta Y., Toh H., Kondo H., Tanabe T.; S. pombe ACCl, Submitted (Dec-1996) to Embl/Genbank/Ddbj Data Banks. Schulte W., Schell J., Toepfer R.; Plant Physiol. 106:793-794(1994). Sherlock G., Bahman A. M., Mahal A., Shieh J. C, Fewrreira M., Rosamond J.; Mol. Gen. Genet. 245: 716-723. Takai T., Yokoyama C, Wada K., Tanabe T.; J. Biol. Chem. 263:2651-2657(1988). Wach A., Brachat A., Poehlmann R., Philippseen P.; Yeast 10: 1793-1808 (1994) TABLE 1 Comparative properties of native and recombinant acetyl-CoA carboxylase enzymes Yeast strain Cell Growth Liquid MIC IC50 for Specific doubling temperature activity time for ACCase (μg/ml) Soraphen A of ACCase (minutes) preparation for (nM) preparation (°C) Soraphen A against (nmoles ACCase product/ preparations min/mg protein) C. albicans 56 37 0.003 B2630 S. cerevisiae Mey 160 30 8 0.641 134 S. cerevisiae 163 30 2 2.499 3.054 CLS2-13 S. cerevisiae PNS 253 30 2 17.518 7.025 117a 5C S. cerevisiae PNS 222 30 4 13.083 10.573 117b 6A S. cerevisiae PNS 303 30 0.5 ND 0.244 120a 6C S. cerevisiae PNS 287 30 0.125 ND ND 120b 1C Key :- ND = not determined

Claims: Claims: 1. A polynucleotide encoding .an Acetyl-COA-carboxylase (ACCase) gene from Candida albicans. 2. A polynucleotide as claimed in claim 1 and as set out in Figure 4 herein. 3. A polynucleotide as claimed in claim 2 and characterised by the start codon atg2. 4. A polynucleotide comprising a restriction fragment of a polynucleotide as claimed in any one of claims 1-3. 5. A polynucleotide probe comprising a polynucleotide as claimed in any one of claims 1-4. 6. An Acetyl-COA-carboxyl-ase (ACCase) polypeptide from Candida albicans in isolated and purified form. 7. A polypeptide as claimed in claim 6 and as set out in Figure 5. 8. A polypeptide as claimed in claim 7 and characterised by Met2. 9. A polypeptide as claimed in claim 6 and obtained by expression of a polynucleotide as claimed in any one of claims 1-4. 10. Antibodies specific for a polypeptide as claimed in any one of claims 6-9. 11. An antisense polynucleotide specific for all or a part of a polynucleotide as claimed in any one of claims 1-4. 12. An RNA transcript corresponding to a polynucleotide as claimed in any one of claims 1-4. 13. An expression system for expression of an Acetyl-COA-carboxylase (ACCase) 5 polypeptide from Candida albicans which system comprises .an S. cerevisiae host strain having a Candida albicans ACCl polynucleotide as claimed in any one of claims 1-3, inserted in place of the native ACCl gene from S. Cerevisiae, whereby the Candida albicans ACCl polypeptide is expressed. 10 14. An expression system as claimed in claim 13 and adapted for controlled overexpresssion of the Candida albicans polynucleotide relative to expression under the control of a Candida albicans promoter 15. An expression system as claimed in claim 14 and used to provide an Acetyl-COA- 15 carboxylase (ACCase) gene from Candida albicans in sufficient quantity .and with sufficient activity for compound screening puφoses. 16. Use of an Acetyl-COA-carboxylase (ACCase) polypeptide from Candida albicans as claimed in claim 6, in an assay to identify inhibitors of the polypeptide. 0 17. Use as claimed in claim 16 in pharmaceutical research.

Inventors: CHAVDA SUBERNA, DIXON GRAHAM KEITH, SCHNELL NORBERT FRIEDEMANN, THAIN JOHN LESLIE, VINCENT JOHN PHILIP, CHAVDA, SUBERNA, DIXON, GRAHAM, KEITH, SCHNELL, NORBERT, FRIEDEMANN, THAIN, JOHN, LESLIE, VINCENT, JOHN, PHILIP
Applicants: CHAVDA SUBERNA, DIXON GRAHAM KEITH, SCHNELL NORBERT FRIEDEMANN, THAIN JOHN LESLIE, VINCENT JOHN PHILIP, ZENECA LTD, CHAVDA, SUBERNA, DIXON, GRAHAM, KEITH, SCHNELL, NORBERT, FRIEDEMANN, THAIN, JOHN, LESLIE, VINCENT, JOHN, PHILIP, ZENECA LIMITED
Application year: 1998
Main classification: C12N15
IPC codes: A61K45, A61P31, C07K16, C12N1, C12N9, C12N15, C12Q1