PHARMACEUTICALLY AVAILABLE PROTOBERBERINE SALTS DERIVATIVES, AND PROTOBERBERINE DERIVATIVES AND SALTS THEREOF

Abstract: The novel compounds of chemical formulae (I) and (II) exhibit in vitro antifungal activity against fungi including cutaneous filamentous fungus, such as Epidermophyton, Microsporum, Trichophyton, Sporothrix schenckii, Aspergillus or Candida. The compounds of the present invention exhibit in vitro antifungal activity at the concentration of 1-100 mu g/ml, in formulae (I) and (II) R<1>, R<2>, and R<4> may be the same or different, and represent C1-C5 alkoxy, R<3> represents hydrogen or C1-C10 alkyl, A<-> represents inorganic acid ion, organic acid ion or halide, R<5> represents hydrogen, pyridylmethyl, substituted pyridylmethyl or a group having chemical formula (XI), wherein Z<1>, Z<2>, Z<3>, Z<4> and Z<5> may be the same or different, and independently of one another represent hydrogen, halogen, C1-C5 alkyl, trifluoromethyl, phenyl, substituted phenyl, nitro, C1-C4 alkoxy, trifluoromethoxy, hydroxy, phenoxy, vinyl, methoxycarboxyl groups.

Description: PHARMACEUTICALLY AVAILABLE PROTOBERBERINE SALTS DERIVATIVES, AND PROTOBERBERINE DERIVATIVES AND SALTS THEREOF Technical Field The present invention relates to a pharmaceutically available protoberberine salt derivative and to protoberberine derivatives and salts thereof. More particularly, the present invention relates to protoberberine salt derivatives, which have the following chemical formula (I) and the reduction product thereof, i.e., 7,8- dihydroprotoberberine derivative and salt thereof 00 wherein R R 2 and R 4 may be the same or different, and represent - alkoxy, R 3 represents hydrogen or C t -C, 0 alkyl, A ' represents inorganic acid ion, organic acid ion or halide, R 5 represents hydrogen, pyridylmethyl, substituted pyridylmethyl or a group having the following chemical formula (XI) z 2 z 1 z3~ z 4 z ~CH2 (XT) 5 wherein Z 1 , Z", Z\ Z 4 and Z J may be the same or different, and independently of 2 one another srepresent hydrogen, halogen, -C, alkyl, trifruoromethyl, phenyl. substituted phenyl, nitro, C 1 -C alkoxy, trifluoromethoxy, hydroxy, phenoxy, vinyl, methoxycarbonyl groups. Background Art Huanglian, Coptis spp., a traditional drug in the oriental region, is the root of barberry shrub and has been used as a drug for conjunctivitis, diarrhoea etc., from ancient times. The present invention provides novel compounds having antibiotic activities, which is related to berberine, i.e., alkaloid component of Huanglian, Coptis spp. Berberine chloride exhibits antibacterial activity against Gram -negative or Grαw-positive bacteria, such as Staphylococcus aureus, Staphyiococcus hemolyticus, Salmonella typhosa, Shigella dysenteήae, Shigella paradysenteriae, Escherichia coli, Neisseria gonorrhoeae or Diplococcus pneumoniae [Ukita T, Mizuno D. Tamura T, Jpn J Exp Med 20, 103 (1949)]. It has been reported that quaternary ammonium salt of berberine is prerequisite to the above antibacterial activity, and that the derivatives which do not contain such quaternary ammonium salt, for example, tetrahydroberberine exhibits less effect [Pitea. M, Margineanu C, Clujul Med 45, 465(1972)]. In addition, it has been reported that berberine sulfate inhibits the growth of fungi, such as Λlternaria sp., Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Curvularia sp., Drechslera sp., Fusarium sp., Mucor sp., Penicillium sp., Rhizopus oryzae, or Scopulariopsis sp. at 10-25mg/ml [Mahajan V M, Shar a A, Rattan A, Sabouraudia 20, 79 (1982)], and that berberine sulfate is also effective in case of oral administration of 350-700mg/kg for mice infected with Candida albicans [Mirska I, Kedzia H, Kowalewski Z, Kedzia W, Arch Immunol. Ther Exp 20, 921 (1972)]. 3 However, the antifungal activities of the compounds disclosed in the above prior arts are too weak. Moreover, berberine sulfate exhibits intense toxicity and therebv mav cause a harmful or lethal effect on human in case of being dosed excessively. Accordingly, the present invention is contrived to solve these disadvatages. Disclosure of Invention On the basis of the above mentioned prior art, the present inventors have prepared novel berberine analogous compounds from the berberine compound, and as a result, have found that these novel compounds exhibit fungicidal effect or growth-inhibitory effect against fungi including cutaneous filamentous fungus, such as Epidermophyton, Microsporum, Trichophyton, Sporothrix schenckii, Aspergillus or Candida. Therefore, it is an object of the present invention to provide protoberberine salts derivatives which exhibit fungicidal effect and/or growth-inhibitory effect against fungi. Another object of the present invention is to provide a pharmaceutically available 7,8-dihydroprotoberberine derivative and salt thereof, i.e., reduction product of the above-described protoberberine derivative. Yet another object of the present invention is to provide a pharmaceutical formulation containing pharmaceutically effective amount of the above protoberberine salts derivative or of its reduction product and salts thereof. A still further object of the present invention is to provide an antifungal formulation containing pharmaceutically effective amount of the above protoberberine salt derivative or of its reduction product and salts thereof. A still further object of the present invention is to provide processes for preparing the above-described protoberberine salts derivatives, and its reduction product and salts thereof, i.e., 13-substituted berberine salt and 13-substituted palmatine salt. The above objects of the present invention are achieved by providing the protoberberine salt derivatives of the following chemical formula (I) (I) wherein R 1 , R 2 , and R 4 may be the same or different, and represent - alkoxy, R 3 represents hydrogen or C -C 10 alkyl, A " represents inorganic acid ion, organic acid ion or halide, more particularly, nitrate, sulfate, acetate, tartrate, maleate, succinate, citrate, fumarate, aspartate, sahcylate, glycerate, ascorbate, fluoride, chloride, iodide or bromide, R 5 represents hydrogen, pyridylmethyl, substituted pyridylmethyl or a group having the following chemical formula (XI) z 2 z 1 23 ^ CH 2 Z 4 Z 5 wherein Z 1 , Z 2 , Z 3 , Z 4 and Z 5 may be the same or different, and represent hydrogen, halogen, -C 5 alkyl, trifluoromethyl, phenyl, substituted phenyl, nitro, - alkoxy, trifluoromethoxy, hydroxy, phenoxy, vinyl, methoxycarbonyl groups. Another object of the present invention is achieved by providing a pharmaceutically available 7,8-dihydroprotoberberine derivative and salts thereof, i.e., the reduction product of the above-described protoberberine derivative of the following chemical formula(II) (II) wherein R\ R , R , R and R 5 are as defined above. Another object of the present invention is achieved by providing a pharmaceutical formulation which contains pharmaceutically effective amount of the compounds of the above chemical formulae (I) or (II), and contains pharmaceutically allowable excipient or vehicle. Another object of the present invention is achieved by providing an antifungal formulation which contains pharmaceutically effective amount of the compounds of the above chemical formulae (I) or (II), and contains pharmaceutically allowable excipient or vehicle. Another object of the present invention is achieved by providing processes for preparing 13-substituted berberine salt or 13-substituted palmatine salt of the following chemical formula (V), in which: 1.0 Mol of berberine salt or palmatine salt of the following chemical formulae (III) is reacted with 1.0 to 3.0 mol of NaBH 4 and 2.0 to 4.0 mol of potassium carbonate in alcohol solvent to prepare dihydroberberine or dihydropalmatine of the following chemical formula (VI); The compound thus obtained is reacted with 1.0 to 3.0 mol of electrophiles (R 5 -X) in organic solvent to prepare 13-alkyl-dihydroberberine compound or 13- alkyl-dihydropalmatine of the following chemical formula (VII); and The compound thus obtained is oxidized with N-QιlorosucciDimide(NCS) or N-bromosuccinimide to give the above-mentioned 13-substituted berberine salt or 13-substituted palmatine salt. 3 (III) OCH 3 (VI) (VII) (V) wherein R 1 , R 2 , R 5 are as defined above and X represents inorganic acid ion, organic acid ion or halide, more particularly, nitrate, sulfate, acetate, tartrate, maleate, succinate, citrate, fumarate, aspartate, sahcylate, glycerate, ascorbate, fluoride, chloride, iodide or bromide. 7 Toe present invention is further explained hereinbelow. The present invention provides protoberberine(5.6-dihydro-dibenzo a.g - quinclizinium) salt derivatives of the above chemical formula < I) and pharmaceutically available 7.S-dihydroprotoberberine tertiary amine derivatives and salt thereof, i.e., the reduction product of the protoberberine derivatives of the above chemical formula (II). In general, according to the process described in U.K. patent No. 125,627, the novel compounds of the above chemical formulae (I) and (II) of the present invention are prepared from berberine salt or palmatine salt of the following chemical formula (III) by the process of the following reaction scheme(la). The following reaction scheme (la) represents the reaction steps for the preparation of novel protoberberine derivatives, Nos 1 to 6, 15 to 19, 62 to 63, 66 to 77 of the following Table 1, in which: 1.0 Mol of berberine salt or palmatine salt of the above formula (III) is reacted with 3.5 to 11.5 mol of NaOH and 2.5 to 13.5 mol of acetone to obtain 8- acetonyldihydro berberine or 8-acetonyldihydropalmatine of the following chemical formula (IV); and The compound of the following chemical formula (IV) is reacted with 2.5 to 7.5 mol of electrophiles (R J -X) under reflux in organic solvent to obtain 13- alkylberberine salt or 13-alkylpalmatine salt, Reaction scheme (la) R l - R J — X RJ OCH 3 :H I (III) (IV) (V) wherein R 1 . R 2 . R ; and X " are as defined above. O 99/55704 The novel compounds having the above chemical formulae I and II of the present invention, may be prepared by processes of the following reaction scheme (lb). Reaction scheme (lb) (m) R s -X Oxidation Z (VII) (V) wherein R , R , R and X " are as defined above. The above reaction scheme (lb) represents the course of reaction for the preparation of novel protoberberine derivatives, Nos. 7 to 14, 20 to 35, 58 to 61, 65 and 100 to 102 described in the following Table 1, in which: 1.0 mol of berberine salt or palmatine salt of the following chemical formulae (III) is reacted with 1.0 to 3.0 mol of NaBI^ and 2.0 to 4.0 mol of potassium carbonate in alcohol solvent to prepare dihydroberberine or dihydropalmatine of the following chemical formula (VI); the compound thus obtained is reacted with 1.0 to 3.0 mol of electrophiles (R 5 -X) in organic solvent to prepare 13-alkyldihydroberberine compound or 13- 99/55704 alkyldihydropalmatine of the following chemical formula (VII); and the compound(VII) thus obtained is oxidized with N-chlorosuccinimide (NCS) or N-bromosuccinimide to give 13-substituted berberine salt or palmatine salt of the chemical formula (V). The following reaction scheme (lc) represents the course of reaction for the preparation of novel protoberberine derivative No. 85 described in the following Table 1, in which: 1.0 mol of the compound having the chemical formulae (III) or (V) is reacted with 1.5 to 9.5 mol of Lewis acid, for example, anhydrous aluminiumchloride in aromatic solvent under reflux to prepare 2,3,9, 10-tetrahydroxy compound of the chemical formula (VHI); and the resulting compound is reacted with 4.0 to 15.0 mol of electrophile (R 5 -X) under reflux in organic solvent to obtain protoberberine salt derivatives of the following chemical formula (IX). Reaction scheme (lc) chemical formulae (III) or (V) R 5 -X (VIII) (IX) wherein R 5 and X " are as defined above, R represents - alkyl. The following reaction scheme (Id) represents the course of reaction for the preparation of novel protoberberine derivatives, Nos. 89 to 99 and 103 to 115 described in the following Table 1, in which 1.0 mol of the compound having the chemical formulae (V) or (LX) is reacted with 2.0 to 6.0 mol of LiAlH 4 or 1.0 to 3.0 mol of NaBH 4 and 2.0 to 4.0 mol of potassium carbonate to obtain dihydro O 99/55704 10 protoberberine derivative of the following chemical formula (II). Reaction scheme (Id) chemical formulae (V) or (IX) LiAlH, > > or NaBH 4 /K 2 CO 3 (H) in which R\ R 2 , R 3 , R and R 5 are as defined above. The following reaction scheme (le) represents the course of reaction for the preparation of novel protoberberine derivatives, Nos. 116, 117 and 118 described in the following Table 1, in which 1.0 mol of the compound having the chemical formulae (III) or (V) is reacted with 2.0 to 6.0 mol of alkylmagnesiumhalide(R 3 - MgX) to obtain dihydroprotoberberine derivative of the following chemical formula (II). Reaction scheme (le) chemical formulae (III),(V) or (IX) R 3 -MgX ■ , (H) in which R , R 2 , R 3 , R 4 and R J are as defined above. O 99/55704 11 The following reaction scheme (If) represents the reaction steps for the preparation of the pharmaceutically available salt of the present invention by the substitution of the counter anion of the quaternary ammonium ion to give novel protoberberine derivatives, Nos. 86, 87 and 88 of the following Table 1, in which 1.0 mol of berberine salt or palmatine salt of the above formula (III) is reacted with 2.5 to 6.5 mol of NaOH and 2.5 to 6.5 mol of acetone to obtain 8- acetonyldihydroberberine and the resulting compound is subsequently treated with inorganic acid or organic acid to substitute anion. In the following reaction scheme(lf), it is preferable that A " is inorganic acid ion, organic acid ion or halide, preferably, nitrate, sulfate, acetate, tartrate, maleate, succinate, citrate, fumarate, aspartate, sahcylate, glycerate, ascorbate. Reaction scheme(lf) H-A (V) or (IV) (X) (I) in which R 1 , R 2 , R 4 and R 5 are as defined above, R 3 represents hydrogen, A " and X are different each other and represent inorganic acid ion, organic acid ion or halide, more particularly, nitrate, sulfate, acetate, tartrate, maleate, succinate, citrate, fumarate, aspartate, salicylate, glycerate, ascorbate, fluoride, chloride, iodide or bromide Meanwhile, among the compounds having the above chemical formula (I), the compound wherein R*-R 2 , R 3 , R 4 and R s are methylenedioxy(-O-CH 2 -O-), hydrogen, methoxy and 3,4-dimethylbenzyl respectively, the compound wherein R l -R 2 , R 3 , R 4 and R J are methylenedioxy(-O-CH 2 -O-), hydrogen, methoxy and 4- 12 (tert-butyl)benzyl respectively, the compound wherein R 1 , R 2 , R 3 , R 4 and R' are methoxy, methoxy, hydrogen, methoxy and 4-(tert-butyl)benzyl respectively, the compound wherein R 1 , R 2 , R 3 , R 4 and R 5 are methoxy, methoxy, hydrogen, methoxy and 4-isopropylbenzyl respectively, the compound wherein R 1 , R 2 , R 3 , R 4 and R 5 are methoxy, methoxy, hydrogen, methoxy and 4-phenylbenzyl respectively, and the compound wherein R 1 , R 2 , R 3 , R 4 and R 5 are propoxy, propoxy, hydrogen, propoxy and hydrogen respectively, exhibit preferable pharmaceutical efficacy. Among the compounds having the above chemical formula (II), the compound wherein R^R 2 , R 3 , R 4 and R 5 are methylenedioxy(-0-CH 2 -0-), octyl(- C 8 H 17 ), methoxy and hydrogen respectively, or the compound wherein R 1 , R 2 , R 3 , R 4 and R 5 are propoxy, propoxy, hydrogen, propoxy and hydrogen respectively, exhibit preferable pharmaceutical efficacy. According to the present invention, a pharmaceutical formulation and antifungal formulation which comprises pharmaceutically effective amount of the compounds of the above chemical formulae (I) or (II) and pharmaceutically allowable excipient or vehicle, are provided. Such formulations may be prepared to tablet, syrup or ointment, and may also be dosed by oral delivery, injection, vaginal delivery, dermal application. The effective dosage may be varied within the activity range depend on the amount of above excipient or vehicle. Best Mode for Carrying Out the Invention The present invention will be more specifically illustrated by the following examples but it should be understood that the present invention is not limited to these examples in any manner. The compound number used in the following examples denotes the compound number described in the following Table 1. Example 1: Preparation of 13-benzylberberine iodidefcomp. No. 1) 10G of berberine hydrochloride(an extract of Huanglian, Coptis spp.), 40 ml of water, 10 ml of acetone, 15 ml of 50% agueous NaOH was introduced into a 13 reaction vessel. The reaction mixture was stirred vigorously for 30 minutes. The resulting solid was filtered, washed twice with 10 ml of 80% methanol and then dried to give 8.5g of 8-acetonylhydroberberine. 5.0 G of 8-acetonylberberine was dissolved in 30ml of acetonitrile, and 15ml of benzylchloride and lOg of Nal were added thereto. The reaction mixture was refluxed for 3 hours and concentrated, and then purified by chromatography over silica gel eluting with methanol/ dichloromethane (1:50), to give 3.0g of 13-benzylberberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.25 (t, 2H), 4.08(s, 3H), 4.17(s, 3H), 4.80(s, 2H), 4.91(t, 2H), 6.17(s, 2H), 6.90(s, IH), 7.01(m, 2H), 7.10(s, IH), 7.17(m, 3H), 7.86(d, IH), 8.17(d, IH), 10.11(s, IH) Example 2: Preparation of 13-(4-chlorobenzyl berberine iodidefcomp. No. 2) 5.0 G of 8-acetonylberberine was dissolved in 30 ml of acetonitrile, 16 ml of 4-chlorobenzylchloride, and 10 g of Nal were added thereto. The reaction mixture was refluxed for 3 hours and concentrated. The reaction mixture thus obtained was adsorbed with Celite, and then purified by chromatography over silica gel eluting withmethanol/dichloromethanee(l:50) togive 2.7gof l3-(4-chlorobenzyl)berberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.21 (t, 2H), 4.09(s, 3H), 4.17(s, 3H), 4.80(s, 2H), 4.91(broad t, 2H), 6.17(s, 2H), 6.90(s, IH), 7.05(d, 2H), 7.17(s, IH), 7.40(d, 2H), 7.86(d, IH), 8.17(d, IH), 9.87(s, IH) Example 3: Preparation of 13-(3-chlorobenzyl berberine iodidefcomp. No. 3) The process of Example 2 was repeated except that 3-Chlorobenzyl chloride was employed in place of 4-chlorobenzyl chloride of Example 2, to obtain 2.8 g of 13-(3-chlorobenzyl)berberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.20(t, 2H), 4.07(s, 3H), 4.17(s, 3H), 4.70(s, 14 2H), 4.85(broad t, 2H), 6.18(s, 2H), 6.90(s, IH), 7.12(s, IH), 7.17(s, IH). 7.31(t, IH), 7.40(d, IH), 7.48(d, IH), 7.76(d, IH), 8.10(d, IH), 10.05 (s, IH) Example 4: Preparation of 13-(2-chlorobenzyl)berberine iodidefcomp. No. 4) The process of Example 2 was repeated except that 2-chlorobenzyl chloride was employed in place of 4-chlorobenzyl chloride of Example 2 to obtain 2.3 g of 13-(2-chlorobenzyl)berberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.20(t, 2H), 4.03(s, 3H), 4.13(s, 3H), 4.65(s, 2H), 4.91(broad t, 2H), 6.09(s, 2H), 6.74(s, IH), 6.80(d, 2H), 7.17(s, IH), 7.24(t,lH), 7.35(t, IH), 7.66(d, IH), 7.70(d, IH), 8.11(d, IH), 10.05(s, IH) Example 5: Preparation of 13-f4-bromobenzyl)berberine iodidefcomp. No. 5) The process of Example 2 was repeated except that 4-Bromobenzyl chloride was employed in place of 4-chlorobenzyl chloride of Example 2 to obtain 2.5 g of 13-(4-bromobenzyl)berberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.21(t, 2H), 4.09(s, 3H), 4.17(s, 3H), 4.80(s, 2H), 4.91(broad t, 2H), 6.17(s, 2H), 6.91(s, IH), 7.13(d, 2H), 7.16(s, IH), 7.55(t, IH), 7.74(t, IH), 8.10(d, IH), 10.05(s, IH) Example 6: Preparation of 13-{3-bromobenzyl)berberme iodidefcomp. No. 6) The process of Example 2 was repeated except that 3-Bromobenzyl chloride was employed in place of 4-chlorobenzyl chloride of Example 2 to obtain 2.8 g of 13-(3-bromobenzyl)berberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.20(t, 2H), 4.07(s, 3H), 4.17(s, 3H), 4.70(s, 2H), 4.85(broad t, 2H), 6.10(s, 2H), 6.91(s, IH), 7.15(d, 2H), 7.17(s, IH), 7.31(t, IH), 7.48(d, IH), 7.50(d, IH), 7.76(d, IH), 8.09(d, IH), 15 10.02(s, IH) Example 7: Preparation of 13-(2.3-dichlorobenzyl)berberine chloridef comp. No. 7) To a solution of 9.27 g (20 mmol) of berberine hydrochloride(an extract of Huanglian, Coptis spp.) in 250ml of methanol was added to 8.29g(60mol) of K 2 C0 3 and then 0.24g(6.4mmol) of NaBH 4 was slowly added thereto. The reaction was proceeded for 4 hours at 0°C. The reaction mixture was concentrated and then the solid thus obtained was filtered, washed successively with 50ml of distilled water until it become to be neutral and dried under vacuo to give 6.61g of 8-dihydroberberine(yield: 98%). A mixture of 2.70 g(18 mmol) of Nal and 4.32 g(18 mmol) of 2,3-dichlorobenzyl bromide in 150ml of acetonitrile was stirred at room temperature for 1 hour and then 5.06 g(15 mmol) of 8-dihydroberberine obtained in the above, was added to the reaction mixture and heated for 3 hours under reflux. The reaction mixture was concentrated and dissolved in 100ml of chloroform and then quenched with 10% aqueous solution of K 2 C0 3 to separate organic layer. The organic layer thus separated was dried over MgS0 4 and filtered. The filtrate was concentrated to an extent of 100ml and 2.60 g(18 mmol) of NCS (N-chlorosuccinimide) was added thereto. The reaction was proceeded at room temperature for 1 hour. The reaction mixture was washed with 100 ml of distilled water. The organic layer was separated and dried over MgS0 4 and filtered. The filtrate was concentrated. The solid thus obtained was triturated in 20ml of diethylether and filtered to give 4.20g of 13-(2,3-dichlorobenzyl)berberine chloride. (Melting point: 116'C) NMR(300MHz, CDC ) 5: 3.24-3.28(broad t, J=5.7Hz, 2H), 4.04(s, 3H), 4.42(s, 3H), 4.68(s, 2H), 5.20-5.24(broad t, J=5.7Hz, 2H), 6.04(s, 2H), 6.74-6.77(m, 2H), 6.90(s, IH), 7.11-7.16(t, J=8.1Hz, IH), 7.41-7.49(m, 2H), 7.73-7.70(d, J=9.3Hz, IH), 10.55 (s, IH) Example 8: Preparation of 13-f2-fhιorobenzyl)berberine chloridef comp. No. 8) 16 Nal(2.70 g, 18 mmol) was dissolved in 150ml of acetonitrile and then 2.60 g (18 mmol) of 2-fmorobenzyl chloride was added thereto and reacted at room temperature for 1 hour. 8-Dihydroberberine (5.068 g, 15 mmol) was added to the resulting solution and the mixture was heated under reflux for 3 hours. The reaction mixture thus obtained was concentrated and dissolved in 100 ml of chloroform and then quenched with 10% aqueous solution of K 2 C0 3 to separate organic layer. The organic layer thus separated was dried over MgS0 4 and filtered. The filtrate was concentrated to an extent of 100 ml and 2.60 g (Iδmmol) of NCS (N-chlorosuccinimide) was added thereto and reacted at room temperature for 1 hour. The reaction mixture was washed with 100 ml of distilled water. The organic layer was separated and dried over MgS0 and filtered. The filtrate was concentrated. The solid thus obtained was triturated in 20 ml of diethylether and filtered to give 4.06 g of 13-(2-fluorobenzyl)berberine chloride. (Melting point: 177°C) NMR(300MHz, CDC1 3 ) 8: 3.23-3.27(broad t, J=5.7Hz, 2H), 4.03(s, 3H), 4.41(s, 3H), 4.63(s, 2H), 5.36-5.40(broad t, J=5.7Hz, 2H), 6.02(s, 2H), 6.71-6.77(m, IH), 6.87(s, IH), 6.90(s, IH), 7.02-7.07(m, IH), 7.20-7.36(m, 2H), 7.54-7.57(d, J=9.3Hz, IH), 7.69-7.72(d, J=9.3Hz, IH), 10.88(s, IH) Example 9: Preparation of 13-f2-chloro-6-fluoro benzyl) berberine chloridefcomp. No. 9) The process of Example 8 was repeated except that 2-chloro-6-fhiorobenzyl chloride was employed in place of 2-fluorobenzyl chloride of Example 8 to give 3.98 g of 13-(2-chloro-6-fluorobenzyl)berberine chloride. (Melting point: 80°C) NMR(300MHz, CDC1 3 ) 8: 3.26-3.30(broad t, J=5.7Hz, 2H), 4.03(s, 3H), 4.32(s, 3H), 4.89(s, 2H), 5.05-5.09(broad t, J=5.7Hz, 2H), 6.09(s, 2H), 6.88-6.91(m, IH), 6.94(s, IH), 7.10(s, IH), 7.18-7.24(m, 2H), 7.72(s, 2H), 10.11(s, IH) 17 Example 10: Preparation of 13-f2.6-difluorobenzyl berberine chloridefcomp. No. m The process of Example 8 was repeated except that 2,6-difluorobenzyl chloride was employed in place of 2-fluorobenzyl chloride of Example 8 to give 4.11 g of 13-(2,6-difluorobenzyl)berberine chloride. (Melting point: 94°C) NMR(300MHz, CDC1 3 ) 8: 3.26-3.30(broad t, J=5.7Hz, 2H), 4.04(s, 3H), 4.32(s, 3H), 4.80(s, 2H), 5.14-5.18(broad t, J=5.7Hz, 2H), 6.09(s, 2H), 6.83-6.88(t, /=8.4Hz, 2H), 6.95(s, IH), 7.11(s. IH), 7.19-7.26(m, IH), 7.73-7.76(d, J=9.3Hz, IH), 7.77-7.80(d, /=9.3Hz, IH), 10.18(s, IH) Example 11: Preparation of 13-(3.4-difluorobenzyl berberine chloridefcomp. No. m The process of Example 8 was repeated except that 3,4-difluorobenzyl chloride was employed in place of 2-fluorobenzyl chloride of Example 8 to give 4.07 g of 13-(3,4-difluorobenzyl)berberine chloride. (Melting point: 88 °C) NMR(300MHz, CDC1 3 ) 5: 3.25-3.29(broad t, J=5.7Hz, 2H), 4.03(s, 3H), 4.29(s, 3H), 4.63(s, 2H), 5.03-5.07(broad t,J=5.7Hz, 2H), 6.03(s, 2H), 6.84(s, IH), 6.88(s, IH), 7.08-7.10(m, IH), 7.51-7.54(d. J=9.3Hz, IH), 7.69-7.74(m, 3H), 10.52(s, IH) Example 12: Preparation of 13-(4-fluoro-2-trifluoromethylbenzyl)berberine chloridefcomp. No. 12) The process of Example 8 was repeated except that 4-fluoro-2- trifluoromethylbenzyl chloride was employed in place of 2-fluorobenzyl chloride of 18 Example 8 to give 4.31 g of 13-(4-fluoro-2-trifluoromethylbenzyl)berberine chloride. (Melting point: 127°C) NMR(300MHz, CDC1 3 ) 8: 3.25-3.29(broad t, J=5.7Hz, 2H), 4.05(s, 3H), 4.36(s, 3H), 4.80(s, 2H), 5.14-5.18(broad t,J=5.7Hz, 2H), 6.03(s, 2H). 6.78(s, IH), 6.90(s, IH), 7.01-7.06(m, IH), 7.15-7.22(m, IH), 7.47-7.50(d, J=9.6Hz, IH), 7.55-7.60(dd, J=2.7, 9.0 Hz, IH), 7.73-7.76(d, J=9.6Hz, IH), 10.28(s, IH) Example 13: Preparation of 13-(2.3,4.5,6-pentafluorobenzyl)berberine chloride fcomp. No. 13) The process of Example 8 was repeated except that 2,3,4,5,6- pentafluorobenzyl chloride was employed in place of 2-fluorobenzyl chloride of Example 8 to give 4.14 g of 13-(2,3,4,5,6-pentafluorobenzyl) berberine chloride. (Melting point: 95 °C) NMR(300MHz, CDC1 3 ) 8: 3.26-3.30(broad t, J=5.7Hz, 2H), 4.07(s, 3H), 4.32(s, 3H), 4.89(s, 2H), 4.97-5.01(broad t,J=5.7Hz, 2H), 6.11(s, 2H), 6.96(s, IH), 7.09(s, IH), 7.70-7.74(d, J=9.6Hz, IH), 7.81-7.85(d, J=9.6Hz, IH), 10.01(s, IH) Example 14: Preparation of 13-f2.3.5,6-tetrafluoro-4-trifluoroethylbenzyl) berberine chloride fcomp. No. 14) The process of Example 8 was repeated except that 2,3,5,6-tetrafluoro-4- trifluoromethylbenzyl chloride was employed in place of 2-fluorobenzyl chloride of Example 8 to give 4.36 g of 13-(2,3,5,6-tetrafluoro-4-trifiuoromethylbenzyl) berberine chloride. (Melting point: 150 °C) NMR(300MHz, CDC1 3 ) 8: 3.23-3.27(broad t, J=5.7Hz, 2H), 4.07(s, 3H), 19 4.35(s, 3H), 4.93(s, 2H).5.17-5.21(broad t,J=5.7Hz, 2H), 6.11(s.2H), 6.97(s, IH), 7.01(s, IH), 7.62-7.65(d, J=9.0Hz, IH), 7.81-7.84(d, J=9.0Hz, IH), 10.5 l(s, IH) Example 15: Preparation of 13-(4-methylbenzyl)berberine chloridefcomp. No. 15) 4-Methylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.2 g of 13-(4-methyl benzyl)berberine chloride. NMR(300MHz, DMSO-d 6 ) 8: 2.23(s, 3H), 3.21(t, 2H), 4.09(s, 3H), 4.17(s, 3H), 4.60(s, 2H), 4.82(broad t, 2H), 6.05(s, 2H), 6.97(s, 2H), 7.06(d, 2H), 7.17(s, IH), 7.18(d, 2H), 7.82(d, IH), 8.25(d, IH), 10.05(s, IH) Example 16: Preparation of 13-f3-methylbenzyl)berberine chloridefcomp. No. 16) 3-Methylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.10 g of 13-(3- methylbenzyl)berberine chloride. NMR(300MHz, DMSO-d 6 ) 8: 2.27(s, 3H), 3.20(t, 2H), 4.03(s, 3H), 4.13(s, 3H), 4.70(s, 2H), 4.95(broad t, 2H), 6.09(s, 2H), 6.92(d, IH), 7.00(s, 2H), 7.07(d, IH), 7.11(d, IH), 7.18(s, IH), 7.24(t, IH), 7.76(d, IH), 8.10(d, IH), 10.05(s, IH) Example 17: Preparation of 13-(2-methylbenzvI)berberine chloridefcomp. No. 17) 2-Methylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.5 g of 13-(2-methyl benzyl)berberine chloride. NMR(300MHz, DMSO-d 6 ) 8: 2.25(s, 3H), 3.18(t, 2H), 4.05(s, 3H), 4.17(s, 20 3H), 4.55(s, 2H). 4.95(broad t, 2H), 6.05(s, 2H), 6.61(d, IH), 6.79(s, 2H), 7.06(t, IH), 7.16(s. IH), 7.22(t, IH), 7.40(d, IH), 7.72(d, IH). 8.11(d. IH), 10.05 (s, IH) Example 18: Preparation of 13-(3,4-dimethylbenzyl)berberine chloridefcomp. No. 3.4-Dimethylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.7 g of 13-(3,4- dimethylbenzyl)berberine chloride. NMR(300MHz, DMSO-d 6 ) 8: 2.18(s, 3H), 2.20(s, 3H), 3.21(t, 2H), 4.02(s, 3H), 4.13(s, 3H), 4.65(s, 2H), 4.91(broad t, 2H), 6.08(s, 2H), 6.81(d, IH), 7.01(s, IH), 7.02(d, IH), 7.10(d, IH), 8.11(d, IH), 10.05(s, IH) Example 19: Preparation of 13-(2.4-dimethylbenzyl)berberine chloridefcomp. No. 191 2,4-Dimethylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.4 g of 13-(2,4- dimethylbenzyl)berberine chloride. NMR(300MHz, DMSO-d 6 ) 8: 2.27(s, 3H), 2.42(s, 3H), 3.20(t, 2H), 4.07(s, 3H), 4.17(s, 3H), 4.50(s, 2H), 4.90(broad t, 2H), 6.08(d, IH), 6.50(d, IH), 6.81(s, IH), 6.88(dd, IH), 7.16(s, IH), 7.21(s, IH), 8.27(d, IH), 10.05(s, IH) Example 20: Preparation of 13-f2,5-dimethylbenzyl)berberine chloridefcomp. No. 20) 2,5-Dimethylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.31 g of 13-(2,5- dimethylbenzyl)berberine chloride. 21 (Melting point: 79°C) NMR(300MHz, CDC1 3 ) 8: 2.14(s, 3H), 2.41(s, 3H), 3.22-3.26(broad t, /=5.7Hz, 2H), 4.06(s, 3H), 4.37(s, 3H), 4.48(s, 2H), 5.06-5.10(broad t, J=5.7Hz, 2H), 6.01(s, 2H), 6.45-6.46(d, 7= 2.1Hz, IH), 6.89(s, IH), 6.90(s, IH), 7.04-7.06(dd, 7=2.1, 7.8Hz, IH), 7.24-7.26(d, 7=7.8Hz, IH), 7.56-7.60(d, 7=9.3Hz, IH), 7.71-7.75(d, 7=9.3Hz, IH), 10.18(s, IH) Example 21: Preparation of 13-f5-methyl-2-nitrobenzyl)berberine chloridefcomp. No. 21) 5-Methyl-2-nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.20 g of 13- (5-methyl-2-nitrobenzyl)berberine chloride. (Melting point: 145 °C) NMR(300MHz, CDC1 3 ) 8: 2.35(s, 3H), 3.23-3.27(broad t, 7=5.7Hz, 2H), 4.06(s, 3H), 4.37(s, 3H), 5.02(s, 2H), 5.01-5.04(broad t,7=5.7Hz, 2h), 6.02(s, 2H), 6.84(s, IH), 6.89(s, IH), 6.98-6.99(d, 7=2.1Hz, IH), 7.29-7.32(dd, 7=2.1, 8.4Hz, IH), 7.49-7.53(d, 7=9.3Hz, IH), 7.74-7.77(d, 7=9.3Hz, IH), 8.16-8.19(d, 7 =8.4Hz, IH), 9.99(s, IH) Example 22: Preparation of 13-(4-methyl-3-nitrobenzyl)berberine chloridefcomp. No. 22) 4-Methyl-3-nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.13g of 13- (4-methyl-3-nitrobenzyl)berberine chloride. (Melting point: 134°C) NMR(300MHz, CDC1 3 ) 8: 2.61(s, 3H), 3.33-3.37(broad t, 7=5.7Hz, 2H), 4.06(s, 3H), 4.35(s, 3H), 4.80(s, 2H), 4.98-5.02(broad t,7=5.7Hz, 2H), 6.04(s, 22 2H), 6.87(s, IH), 6.91(s, IH), 7.26-7.40(m, 2H), 7.58-7.61(d, 7=9.0Hz. IH), 7.75-7.78(m, 2H), 9.99(s, IH) Example 23: Preparation of 13-(2-methyl-3-nitrobenzyl) berberine chloridefcomp. No. 23) 2-Methyl-3-nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.91 g of 13- (2-methyl-3-nitrobenzyl)berberine chloride. (Melting point: 116°C) NMR(300MHz, CDC1 3 ) 8: 2.59(s, 3H), 3.26-3.30(broad t, 7=5.7Hz, 2H), 4.06(s, 3H), 4.37(s, 3H), 4.62(s, 2H), 5.18-5.22(broad t,7=5.7Hz.2H), 6.03(s, 2H), 6.72(s, IH), 6.91(s, IH), 7.01-7.03(d, 7=7.5Hz, IH), 7.23-7.26(d, 7=8.1Hz, IH), 7.43-7.46(d,7=9.3Hz, IH), 7.72-7.78(t,7=7.5Hz, 2H), 10.41(s, IH) Example 24: Preparation of 13-f2-trifluoromethylbenzyl)berberine chloridefcomp. No. 24) 2-Trifluoromethylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.83 g of 13- (2-trifluoromethylbenzyl)berberine chloride. (Melting point: 141 °C) NMR(300MHz, CDC1 3 ) 8: 3.28-3.32(broad t, 7=5.7Hz, 2H), 4.04(s. 3H), 4.36(s, 3H), 4.85(s, 2H), 5.16-5.20(broad t,7=5.7Hz, 2H), 6.01(s, 2H), 6.81(s, IH), 6.90(s, IH), 6.98-7.02(m, IH), 7.45-7.52(m, 3H), 7.71-7.74(d, 7= 9.6Hz, IH), 7.84-7.88(m, IH), 10.28(s, IH) Example 25: Preparation of 13-f3-trifluoromethylbenzyl)berberine chloridefcomp. No. 25) 23 3-Trifluoromethylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.09 g of 13- (2-trifiuoromethylbenzyl)berberine chloride. (Melting point: 149°C) NMR(300MHz, CDC1 3 ) 8: 3.31-3.35(broad t, 7=5.7Hz, 2H), 4.06(s, 3H), 4.37(s, 3H), 4.81(s, 2H), 5.07-5.11 (broad t, 7=5.7Hz, 2H), 6.03(s, 2H), 6.85(s. IH), 6.92(s, IH), 7.30-7.60(m, 5H), 7.74-7.77(d, 7=9.3Hz, IH), 10.19(s, IH) Example 26: Preparation of 13-(4-trifluoromethylbenzyl)berberme chloridefcomp. No. 26) 4-Trifluoromethylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.27 g of 13- (4-trifluoromethylbenzyl)berberine chloride. (Melting point: 168°C) NMR(300MHz, CDC1 3 ) 8: 3.31-3.35(broad t, 7=5.7Hz, 2H), 4.05(s, 3H), 4.36(s, 3H), 4.81(s, 2H), 5.05-5.09(broad t,7=5.7Hz, 2H), 6.03(s, 2H), 6.86(s, IH), 6.91(s, IH), 7.29-7.32(d, 7=8.4Hz, 2H), 7.55-7.58(d, 7=9.3Hz, IH), 7.63-7.66(d, 7=8.4Hz, 2H), 7.73-7.76(d, 7=9.3Hz, IH), 10.20(s, IH) Example 27: Preparation of 13-f4-methoxybenzyl)berberine chloridefcomp. No.27) 4-Methoxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.38 g of 13-(4- methoxybenzyl)berberine chloride. (Melting point: 214°C) NMR(300MHz, CDC1 3 ) 8: 3.23-3.27(broad t, 7=5.7Hz, 2H), 3.81(s, 3H), 4.03(s, 3H), 4.43(s, 3H), 4.61(s, 2H), 5.17-5.22(broad t,7=5.7Hz, 2H), 6.01(s, 2H), 6.889(s, IH), 6.88-6.91(d, 7=9.0Hz, 2H), 7.00(s, IH), 7.02-7.05(d, 7=9.0Hz, 2H), 7.62=7.65(d, 7=8.4Hz, IH), 7.68-7.71(d, 7=8.4Hz, IH), 24 10.44fs, IH) Example 28: Preparation of 13-(3-methoxybenzyl) berberine chloridefcomp. No.28) 3-Methoxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.30g of 13-(3- methoxybenzyl)berberine chloride. (Melting point: 72°C) NMR(300MHz, CDC1 3 ) 8: 3.30-3.34(broad t, 7=5.7Hz, 2H), 3.79(s, 3h), 4.04(s, 3H), 4.37(s, 3H), 4.68(s, 2H), 5.13-5.17(broad t, 7=5.7Hz, 2H), 6.02(s, 2H), 6.68-6.70(m, 2H), 6.83-6.86(m, IH), 6.90(s, IH), 7.01(s, IH), 7.29-7.32(m, IH), 7.65-7.68(d, 7=9.3Hz, IH), 7.71-7.74(d, 7=9.3Hz, IH), 10.34(s, IH) Example 29: Preparation of 13-f3A5-trimethoxybenzyl)berberine chloridefcomp. No. 29) 3,4,5-Trimethoxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.02 g of 13- (3,4,5-trimethoxybenzyl)berberine chloride. (Melting point: 74°C) NMR(300MHz, CDC1 3 ) 8: 3.23-3.27(broad t, 7=5.7Hz, 2H), 3.75(s, 6H), 3.87(s, 3H), 4.06(s, 3H), 4.35(s, 3H), 4.65(s, 2H), 5.12-5.16(broad t,7=5.7Hz, 2H), 6.03(s, 2H), 6.34(s, 2H), 6.92(s, IH), 7.10(s, IH), 7.68-7.71 (d, 7= 9.0Hz, IH), 7.77-7.80(d, 7=9.0Hz, IH), 10.26(s, IH) Example 30: Preparation of 13-(4.5-dimethoxy-2-nitrobenzyl)berberme chloride fcomp. No. 30) 4,5-Dimethoxy-2-nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.89 g of 13- 25 (4.5-dimethoxy-2-nitrobenzyl)berberine chloride. (Melting point: 114 * C) NMR(300MHz, CDC1 3 ) 8: 3.11-3.15(broad t. 7=5.7Hz, 2H), 3.67(s, 3H), 4.00(s, 3H), 4.05(s, 3H), 4.36(s, 3H), 5.05(s, 2H), 5.10-5.14(broad t,7=5.7Hz, 2H), 6.03(s, 2H), 6.47(s, IH), 6.87(s, IH), 6.89(s, IH), 7.48-7.5 l(d.7= 9.3Hz, IH), 7.72-7.75(d, 7=9.3Hz, IH), 7.87(s, IH), 10.15(s, IH) Example 31 : Preparation of 13-(4-trifluoromethoxybenzyl) berberine chloridefcomp. No. 31) 4-Trifluoromethoxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.08 g of 13- (4-trifluoromethoxybenzyl)berberine chloride. (Melting point: 132-134°C) NMR(300MHz, CDC1 3 ) 8: 3.22-3.26(broad t, 7=5.7Hz, 2H), 4.03(s, 3H), 4.37(s, 3H), 4.69(s, 2H), 5.30-5.34(broad t,7=5.7Hz, 2H), 6.02(s, 2H). 6.86(s, IH), 6.89(s, IH), 7.15-7.17(d, 7=8.1Hz, 2H), 7.22-7.24(d, 7=8.1Hz, 2H), 7.55-7.58(d, 7=9.3Hz, IH), 7.71-7.75(d, 7=9.3Hz, IH), 10.77(s, IH) Example 32: Preparation of 13-f2-methoxy-5-nitrobenzyl) berberine chloridefcomp. No. 32) 2-Methoxy-5-nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.15 g of 13- (2-methoxy-5-nitrobenzyl)berberine chloride. (Melting point: 102°C) NMR(300MHz, CDC1 3 ) 8: 3.38-3.42(broad t, 7=5.7Hz, 2H), 4.06(s, 3H), 4.10(s, 3H), 4.35(s, 3H), 4.66(s, 2H), 5.00-5.04(broad t,7=5.7Hz, 2H), 6.03(s, 2H), 6.77(s, IH), 6.92(s, IH), 7.12-7.15(d, 7=9.3Hz, IH), 7.52-7.56(m, 2H), 2 6 7.75-7.78(d, 9.3Hz, IH), 8.26-8.31(dd, 7=2.7, 9.6Hz, IH), 9.99(s, IH) Example 33: Preparation of 13-f2-nitrobenzyl)berberine chloridefcomp. No. 33) 2-Nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.82 g of 13-(2-nitrobenzyl) berberine chloride. (Melting point: 78 °C) NMR(300MHz, CDC ) 8: 3.11-3.15(broad t, 7=5.7Hz, 2H), 4.05(s, 3H), 4.33(s, 3H), 4.99(s, 2H), 5.01-5.05(broad t, I=5.7Hz, 2H), 6.02(s, 2H), 6.80(s, IH), 6.90(s, IH), 7.20-7.80(m, 4H), 8.06-8.09(d, 7=9.0Hz, IH), 8.20-8.23(d, 7= 9.0Hz, IH), 10.05 (s, IH) Example 34: Preparation of 13-(2-hvdroxy-5-nitrobenzyl) berberine chloridefcomp. No. 34) 2-Hydroxy-5-nitrobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.90 g of 13- (2-hydroxy-5-nitrobenzyl)berberine chloride. (Melting point: 146-147°C) NMR(300MHz, DMSO-d 6 ) 8: 3.14-3.18(broad t, 7=5.7Hz, 2H), 4.05(s, 3H), 4.15(s, 3H), 4.56(s, 2H), 4.88-4.92(broad t,7=5.7Hz, 2H), 6.10(s, 2H), 6.85(s, IH), 7.18(s, IH), 7.22-7.25(d, 7=9.3Hz, IH), 7.51-7.52(d, 7=2.7Hz, IH), 7.75-7.79(d, 7=9.3Hz, IH), 8.11-8.14(m, 2H), 10.08(s, IH), 11.90(s, IH) Example 35: Preparation of 13-(4-phenylbenzyl)berberine chloridefcomp. No. 35) 4-Phenylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.10 g of 13-(4- phenylbenzyl)berberine chloride. 27 (Melting point: 170-172 ° C) NMR(300MHz, CDC1 3 ) 8: 3.28-3.32(broad t, 7= 6.0Hz, 2H), 4.05 (s, 3H), 4.36(s, 3H), 4.78(s.2H), 5.06-5.10(broad t,7=6.0Hz, 2H), 6.02(s, 2H), 6.90(s, IH), 7.05 (s, IH), 7.20-7.60(m, 9H), 7.70-7.73(d, 7=9.0Hz, IH), 7.74-7.78(d, 7=9.0Hz, IH), 10.14(s, IH) Example 36: Preparation of 13-f4-(N.N-dimethylamino)benzyl)berberine cloridefcomp. No. 36) (N,N-dimethylamino)benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.53 g of 13- (4-(N,N-dimethylamino)benzyl)berberine chloride. (Melting point: 152°C) Example 37: Preparation of N'-methylammonium iodidefcomp. No. 37) of 13-(4- fN,N-dimethylamino)benzyl)berberine chloride 4-Chloromethylphenyltrimethylammonium iodide, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.27 g of Ν' -methylammonium iodide of 13-(4-(Ν,Ν-dimethylamino)benzyl) berberine chloride. (Melting point: 167 ° C) Example 38: Preparation of N'-ethylammonium iodidefcomp. No. 38) of 13- 4- (N.N-dimethylamino)benzyl)berberine chloride (4-Chloromethylphenyl)dimethylethylammonium iodide, instead of 2- fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.30 g of Ν'-ethylammonium iodide of 13-(4-(Ν,Ν- dimethylamino)benzyl) berberine chloride. (Melting point: 167°C) 23 Example 39: Preparation of Λ^-benzylammonfum chloridefcomp. No. 39) of 13-(4- fN.N-dimethylamino)benzyl)berberine chloride (4-Chloromethylphenyl)benzyldimethylammonium chloride, instead of 2- fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.41 g of N'-benzylammonium chloride of 13-(4-(N,N- dimethylamino)benzyl) berberine chloride. (Melting point: 178 ° C) Example 40: Preparation of N-hexyl ammonium iodidefcomp. No. 40) of 13-(4- fN.iV-dimethylamino)benzyl)berberine chloride (4-Chloromethylphenyl)dimethylhexylammonium iodide, instead of 2- fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.38 g of N'-hexylammonium iodide of 13-(4-(N,N- dimethylamino)benzyl) berberine chloride. (Melting point: 165 °C) Example 41: Preparation of 13-(2-fluoro-4-t-butylbenzyl)berberine chloride comp. No. 41) 2-Fluoro-4-t-butylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.53 g of 13- (2-fluoro-4-r-butylbenzyl)berberine chloride. (Melting point: 154°C) Example 42: Preparation of 13-f2-nitro-4-t-butylbenzyl)berberine chloridefcomp. No. 42) 2-Nitro-4-t-butylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.03 g of 13- (2-nitro-4-t-butylbenzyl)berberine chloride . 29 (Melting point: 128 °C) Example 43: Preparation of 13-f2-guanidinylbenzyl)berberine chloridefcomp. No. 43) 4-Guanidinylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.11 g of 13-(2- guanidinylbenzyl)berberine chloride. (Melting point: 135 °C) Example 44: Preparation of 13-(4-acetylammobenzyl)berberine chloridefcomp. No. 44) 4-Acetylaminobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.20 g of 13-(4- acetylaminobenzyl)berberine chloride. (Melting point: 105 °C) Example 45: Preparation of 13-(4-carboxylbenzyl)berberine chloridefcomp. No. 45) 4-Carboxylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.17 g of 13-(4- carboxylbenzyl)berberine chloride. (Melting point: 153°C) Example 46: Preparation of 13-f4-(2-hydroxy-2-propyl)benzyl)berberine choridefcomp. No. 46) 4-(2-Hydroxy-2-propyl)benzyl) chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.97 g of 13-(4-(2-hydroxy-2-propyl)benzyl)berberine chloride. (Melting point: 105 °C) 30 Example 47: Preparation of 13-f4-isoρropoxybenzyl)berberine chloridefcomp. No. 4-Isopropoxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8. was treated by the process described in Example 8 to give 4.01 g of 13-(4- isopropoxybenzyl)berberine chloride. (Melting point: 127°C) Example 48: Preparation of 13-f4-(2-butyl)benzyl)berberine chloridefcomp. No.48) 4-(2-Butyl)benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.77 g of 13-(4-(2- butyl)benzyl)berberine chloride. (Melting point: 106°C) Example 49: Preparation of 13-(4-f(2-methyl)-2-butyl)benzyI)berberine chloridefcomp. No. 49) 4-((2-Methyl)-2-butyl)benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.15 g of 13-(4-((2-methyl)-2-butyl)benzyl)berberine chloride. (Melting point: 94°C) Example 50: Preparation of 13-(4-pyrrolidinylbenzyl)berberine chloridefcomp. No. 50) 4-Pyrrolidinylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.15 g of 13-(4- pyrrolidinylbenzyl)berberine chloride. (Melting point: 108 °C) Example 51 : Preparation of 13-f3-carboxylbenzyl)berberine chloridefcomp. No. 51) 3 1 3-Carboxylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.56 g of 13-(3- carboxylbenzyl)berberine chloride. (Melting point: 136 °C) Example 52: Preparation of 13-(4-f 3-pentoxy)benzyl)berberine chloridefcomp. No. 4-(3-Pentoxy)benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.77 g of 13-(4-(3- pentoxy)benzyl)berberine chloride. (Melting point: 135 °C) Example 53: Preparation of 13-(4-etoxycarbonyl)benzyl)berberine chloridefcomp. No. 53) 4-(Etoxycarbonyl)benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.39 g of 13-(4-etoxycarbonylbenzyl)berberine chloride. (Melting point: 110'C) Example 54: Preparation of 13-(4-methylthiobenzyl)berberine chloridefcomp. No. 54) 4-Methylthiobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.01 g of 13-(4- methylthiobenzyl)berberine chloride. (Melting point: 106°C) Example 55: Preparation of 13-f 4-ethylthiobenzyI)berberine chloridefcomp. No.55) 32 4-Ethylthiobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.81 g of 13-(4- ethylthiobenzyl)berberine chloride. (Melting point: 142 ° C) Example 56: Preparation of 13-f4-iodobenzyl)berberine chloridefcomp. No. 56) 4-Iodobenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.01 g of 13-(4- iodobenzyl)berberine chloride. (Melting point: 167 ° C) Example 57: Preparation of 13-f4-fl-hydroxy-2-propyl)benzyl)berberine chloridefcomp. No. 57) 4-(l-Hydroxy-2-propyl)benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 2.98 g of 13-(4-(l-hydroxy-2-propyl)benzyl)berberine chloride. (Melting point: 112°C) Example 58: Preparation of 13- 3-phenoxybenzyl)berberine chloridefcomp. No.58) 3-Phenoxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.98 g of 13-(3- phenoxybenzyl)berberine chloride. (Melting point: 231°C) NMR(300MHz, CDC1 3 ) 8: 3.30-3.34(broad t, 7=5.7Hz, 2H), 4.05(s, 3H), 4.40(s, 3H), 4.66(s, 2H), 5.18-5.22(broad t, 7=5.7Hz, 2H), 6.06(s, 2H), 6.81-7.01(m, 7H), 7.09-7.14(broad t, 7=6.0Hz, IH), 7.30-7.35(m, 3H), 7.63-7.66(d, 7=9.0Hz, IH), 7.72-7.75(d, 7=9.0Hz, IH), 10.43(s, IH) 3 3 Example 59: Preparation of 13-(4-benzyloxybenzyI)berberine chloridefcomp. No. 59) 4-Benzyloxybenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 4.05 g of 13-(4- benzyloxybenzyl)berberine chloride. (Melting point: 176-178°C) NMR(300MHz, CDC1 3 ) 8: 3.28-3.32(broad t, 7=6.0Hz, 2H), 4.05(s, 3H), 4.37(s, 3H), 4.69(s, 2H), 5.05(s, 2H), 5.06-5.10(broad t, 7=6.0Hz, 2H), 6.03(s, 2H), 6.89(s, IH), 6.80-7.43(m, 10H), 7.67-7.70(d, 7=9.3Hz, IH), 7.72-7.75(d, 7=9.3Hz, IH), 10.18(s, IH) Example 60: Preparation of 13-(4-vinylbenzyI)berberine chloride fcomp. No. 60) 4-Vinylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.81g of 13-(4-vinylbenzyl) berberine chloride. (Melting point: 144°C) NMR(300MHz, CDC1 3 ) 5: 3.31-3.35(broad t, 7=5.7Hz, 2H), 4.02(s, 3H), 4.42(s, 3H), 4.66(s, 2H), 5.17-5.21(broad t, 7=5.7Hz, 2H), 5.25-5.30(dd, 7=3.0, 10.8Hz, IH), 5.70-5.79(dd, 7=10.8, 17.1Hz, IH), 6.01(s, 2H), 6.88(s, IH), 6.96(s, IH), 7.08-7.10(d, 7= 8.1Hz, 2H), 7.40-7.43(d, 7= 8.1Hz, 2H), 7.58-7.72(m, 3H), 10.42(s, IH) Example 61: Preparation of 13-(4-methoxy carbonyl benzyl)berberine chloridefcomp. No. 61) 4-Methoxycarbonylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.81 g of 13- (4-methoxycarbonylbenzyl)berberine chloride. 34 (Melting point: 198 ° C) NMR(300MHz, CDC1 3 ) 8: 3.27-3.31(broad t, 7=5.7Hz. 2H), 3.92(s, 3H), 4.04(s, 3H), 4.37(s, 3H), 4.97(s, 2H), 5.16-5.20(broad t,7=5.7Hz, 2H), 6.01(s, 2H), 6.86(s, IH), 6.90(s, IH), 7.23-7.26(d, 7=8.1Hz, 2H), 7.56-7.60(d, 7=9.3Hz, IH), 7.71-7.74(d, 7=9.3Hz, IH), 8.04-8.07(d, 7=8.1Hz, 2H), 10.34(s, IH) Example 62: Preparation of 13-(4-t-butylbenzyl)berberine chloridefcomp. No. 62) 4-t-Butylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.3 g of 13-(4-t- butylbenzyl)berberine choride, or 4-t-butylbenzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.81 g of 13-(4-t-butylbenzyl) berberine chloride. (Melting point: 144'C) NMR(300MHz, DMSO-d 6 ) 8: 1.26(s, 9H), 3.21(t, 2H), 4.09(s, 3H), 4.17(s, 3H), 4.80(s, 2H), 4.91(broad t, 2H), 6.17(s, 2H), 6.99(s, IH), 7.11(d, 2H), 7.16(s, IH), 7.38(d, 2H), 7.78(d, IH), 8.10(d, IH), 10.05(s, IH) Example 63: Preparation of 13-(4- -propylbenzyl)berberine chloridefcomp. No. 63) 4- -propylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 2, was treated by the process described in Example 2 to give 2.3 g of 13-(4~z- propylbenzyl)berberine chloride, or 4-benzyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.40 g of 13-(4- -ρropylbenzyl)berberine chloride. (Melting point: 142°C) NMR(300MHz, DMSO-d 6 ) 8: 1.41(d, 6H), 3.18(t, 2H), 3.40(q, IH), 4.10(s, 3H), 4.16(s, 3H), 4.820(s, 2H), 4.90(broad t, 2H), 6.21(s, 2H), 6.88(s, IH), 35 7.12(d, 2H), 7.16(s. IH), 7.40(d, 2H), 7.75(d, IH), 8.09(d, IH), 10.05fs, IH) Example 64: Preparation of 8-hvdroxy-13-f4-t-butylbenzyl) berberine chloridefcomp. No. 64) To a suspension of 1.0 g(2 mmol) of the compound No. 62 in 10ml of distilled water was added dropwise 0.23g of 30% aqueous of hydrogen peroxide and 0.18 g of 45% NaOH aqueous solution, consecutively and then reacted at room temperature for 1 hour. The solid so precipitated was filtered and dried to give 0.75 g of 8-hydroxy-13-(4-t-butylbenzyl)berberine chloride. NMR(300MHz, CDC1 3 ) 8: 1.32(s, 9H), 3.29-3.33(broad t, 7=5.7Hz, 2H), 4.06(s, 3H), 4.37(s, 3H), 4.73(s, 2H), 4.97-5.01(broad t,7=5.7Hz, 2H), 6.03(s, 2H), 6.90(s, IH), 7.06(s, IH), 7.06-7.08(d, 7= 8.1Hz, 2H), 7.35-7.38(d, 7=8.1Hz, 2H), 7.70-7.73(d,7=9.0Hz, IH), 7.75-7.78(d,7=9.0Hz, IH), 9.91(s, IH) Example 65: Preparation of 13-(2-picolyl)berberine chloride fcomp. No. 65) 2-Picolyl chloride, instead of 2-fluorobenzyl chloride of Example 8, was treated by the process described in Example 8 to give 3.81 g of 13-(2-picolyl) berberine chloride. (Melting point: 122-124°C) NMR(300MHz, CDC1 3 ) 8: 3.30-3.34(broad t, 7=5.7Hz, 2H), 4.00(s, 3H), 4.36(s, 3H), 4.86(s, 2H), 5.12-5.16(broad t,7=5.7Hz, 2H), 6.02(s, 2H), 6.89(s, IH), 7.24-7.26(m, IH), 7.35(s, IH), 7.45-7.48(d, 7=7.8Hz, IH), 7.55-7.58(d, 7=9.6Hz, IH), 7.67-7.71(d, 7=9.6Hz, IH), 7.76-7.83(m, IH), 8.50-8.52(d, 7=3.6Hz, IH), 10.27(s, IH) Example 66: Preparation of 13-benzyl palmatine iodidefcomp. No. 66) 36 A mixture lOg of palmatine hydrochloride, 40ml of water, 15ml of 50% NaOH aqueous solution was stirred vigorously for 30 minutes. The reaction mixture was filtered. The solid thus obtained was washed twice with 20ml of methanol and dried to obtain 7.0g of 8-acetonyl dihydropalmatine. To a solution of 5g of 8-acetonyldihydropalmatine in 30ml of acetonitrile was added 15ml of benzyl chloride and lOg of sodium iodide and then the mixture was heated to reflux for 3 hours. The reaction mixture was concentrated and purified by chromatography over silica gel eluting with methanol/dichloromethane (1:75) to give 3.0g of 13-benzylpalmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.25 (t, 2H), 3.80(s, 3H), 4.03(s, 3H), 4.12(s, 3H), 4.13(s, 3H), 4.80(s, 2H), 4.91(t, 2H), 6.90(s, IH), 7.01(m, 2H), 7.10(s, IH), 7.17(m, 3H), 7.86(d, IH), 8.17(d, IH), 10.11(s, IH) Example 67: Preparation of 13-(4-chlorobenzyl)palmatine iodidefcomp. No. 67) A mixture of 5g of 8-acetonyldihydropalmatine was dissolved in 30ml of acetonitrile, and 15ml of benzyl chloride and lOg of sodium iodide were added thereto and then, refluxed for 3 hours. The reaction mixture was concentrated and adsorbed with Celite, and then Kquid-chromatographed over 60g of silica gel and eluted with methanol/dichloromethane (1:75) to obtain 2.7 g of 13-(4-chloro benzyl)ρalmatine iodide. NMR(300MHz, DMSO-d 6 ) 5: 3.21(t, 2H), 3.81(s, 3H), 4.02(s, 3H), 4.13(s, 3H), 4.14(s, 3H), 4.80(s, 2H), 4.91(broad t, 2H), 6.90(s, IH), 7.05(d, 2H), 7.17(s, IH), 7.42(d, 2H), 7.86(d, IH), 8.17(d, IH), 10.10(s, IH) Example 68: Preparation of 13-f3-chlorobenzyl)palmatine iodidefcomp. No. 68) 3-Chlorobenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 68 to give 2.8 g of 13-(3- chlorobenzyl)palmatine iodide. 37 NMR(300MHz, DMSO-d 6 ) 8: 3.20(t, 2H), 3.81(s, 3H), 4.00(s, 3H), 4.11(s, 3H), 4.12(s, 3H), 4.70(s, 2H), 4.85(broad t, 2H), 6.90(s, IH), 7.12(s, IH), 7.17(s, IH), 7.30(t, IH), 7.40(d, IH), 7.76(d, IH), 8.10(d, IH), 10.15(s, IH) Example 69: Preparation of 13- 2-chlorobenzyl) palmatine iodidefcomp. No. 69) 2-Chlorobenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.3g of 13-(2- chlorobenzyl)palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.20(t, 2H), 3.81 (s, 3H), 4.03(s, 3H), 4.12(s, 6H), 4.65(s, 2H), 4.91(broad t, 2H), 6.74(s, IH), 6.80(d, 2H), 7.17(s, IH), 7.24(t, IH), 7.34(t, IH), 7.68(d, IH), 7.70(d, IH), 8.11(d, IH), 10.15(s, IH) Example 70: Preparation of 13-f4-bromobenzyl) palmatine iodidefcomp. No.70) 4-Bromobenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.5g of 13-(4- bromobenzyl)palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.21 (t, 2H), 3.80(s, 3H), 4.02(s, 3H), 4.13(s, 3H), 4.14(s, 3H), 4.80(s, 2H), 4.91(broad t, 2H), 6.91(s, IH), 7.13(d, 2H), 7.16(s, IH), 7.55(d, 2H), 7.70(d, IH), 8.11(d, IH), 10.05(s, IH) Example 71: Preparation of 13-(3-bromobenzyl)palmatine iodidefcomp. No. 71) 3-Bromobenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.8 g of 13-(3- bromobenzyl)palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 3.20(t, 2H), 3.80(s, 3H), 4.03(s, 3H), 4.14(s, 6H), 4.70(s, 2H), 4.85(broad t, 2H), 6.91(s, IH), 7.15(s, IH), 7.17(s, IH), 38 7.31(t, IH), 7.48(d, IH), 7.50(d, IH), 7.76(d, IH), 8.09(d, IH), 10.02(s, IH) Example 72: Preparation of 13-f4-methylbenzyl)palmatine iodidefcomp. No. 72) 4-Methylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.2 g of 13-(4- methylbenzyl)palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 2.23(s, 3H), 3.21(t, 2H), 3.80(s, 3H), 4.02(s, 3H), 4.13(s, 3H), 4.14(s, 3H), 4.60(s, 2H), 4.82(broad t, 2H), 6.97(s, IH), 7.06(d, 2H), 7.16(s, IH), 7.17(d, 2H), 7.82(d, IH), 8.05(d, IH), 10.05(s, IH) Example 73: Preparation of 13-(3-methylbenzyl)palmatine iodidefcomp. No. 73) 3-Methylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.1 g of 13-(3- methylbenzyl)palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 2.27(s, 3H), 3.20(t, 2H), 3.86(s, 3H), 4.02(s, 3H), 4.11(s, 3H), 4.14(s, 3H), 4.70(s, 2H), 4.95(broad t, 2H), 6.92(d, IH), 7.00(s, IH), 7.07(d, IH), 7.11(d, IH), 7.18(s, IH), 7.24(t, IH), 7.82(d, IH), 7.76(d, IH), 8.11(d, IH), 10.05(s, IH) Example 74: Preparation of 13-f2-methylbenzyl)palmatme iodidefcomp. No. 74) 2-Methylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.5 g of 13-(2- methylbenzyl)palmatme iodide. NMR(300MHz, DMSO-d 6 ) δ: 2.25(s, 3H), 3.18(t, 2H), 3.85(s, 3H), 4.00(s, 3H), 4.11(s, 3H), 4.13(s, 3H), 4.55(s, 2H), 4.95(broad t, 2H), 6.61(d, IH), 6.79(s, 2H), 7.06(t, IH), 7.16(s, IH), 7.22(t, IH), 7.40(d, IH), 7.72(d, IH), 39 8.11(d, IH), 10.05(s, IH) Example 75: Preparation of 13-(3,4-dimethvIbenzyl) palmatine iodidefcomp. No. m 3,4-Dimethylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.7 g of 13-(3,4- dimethylbenzyl) palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 2.18(s, 3H), 2.20(s, 3H), 3.21 (t. 2H), 3.79(s, 3H), 4.02(s, 3H), 4.11(s, 3H), 4.13(s, 3H), 4.65(s, 2H), 4.91(broad t, 2H), 6.81(d, IH), 7.01(s, IH), 7.03(d, IH), 7.10(s, IH), 7.17(s, IH), 7.70(d, IH), 8.10(d, IH), 10.05(s, IH) Example 76: Preparation of 13-(2,4-dimethylbenzyl)ρalmatine iodidefcomp. No. 76) 2,4-Dimethylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.4 g of 13-(2,4- dimethylbenzyl)palmatine iodide. NMR(300MHz, DMSO-d 6 ) 8: 2.27(s, 3H), 2.42(s, 3H), 3.20(t, 2H), 3.78(s, 3H), 4.00(s, 3H), 4.09(s, 3H), 4.11(s, 3H), 4.50(s, 2H), 4.90(broad t, 2H), 6.50(d, IH), 6.80(s, IH), 6.88(dd, IH), 7.16(s, IH), 7.21(d, IH), 7.67(d, IH), 8.10(d, IH), 10.05(s, IH) Example 77: Preparation of 13-f4-t-butylbenzyl)palmatine iodidefcomp. No. 77) 4-t-Butylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.9g of 13-(4-t- butylbenzyl)palmatine iodide. 40 NMR(300MHz, DMSO-d 6 ) δ: 1.26(s, 9H), 3.21(t, 2H), 3.80(s, 3H), 4.00(s, 3H), 4.13(s, 3H), 4.14fs, 3H), 4.91(broad t, 2H), 6.99(s, IH), 7.11(d, 2H), 7.16(s, IH), 7.38(d, 2H), 7.78(d, IH), 8.10(d, IH), 10.05(s, IH) Example 78: Preparation of 13-f4-fiV.iV-dimethylamino)benzyl)palmatine iodidefcomp. No. 78) 4-(N,N-dimethylamino)benzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.8 g of 13-(4-(N,N-dimethylamino)benzyl)palmatine iodide. (Melting point: 149'C) Example 79: Preparation of AT -methylammonium iodide of 13-f4-(N.N-dimethyl amino) benzyl)palmatine iodidefcomp. No. 79) 4-Chloromethylphenyltrimethylammonium iodide, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.7 g of N'-methylammonium iodide of 13-(4-(N,N-dimethylamino)benzyl) palmatine iodide. (Melting point: 151 ° C) Example 80: Preparation of AT -ethyl ammonium iodide of 13-(4-fN.N-dimethyl amino)benzyl)palmatine iodidefcomp. No. 80) (4-Chloromethylphenyl)dimethyethyllammonium iodide, instead of 4- chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 3.0 g of N'-ethylammonium iodide of 13-(4-(N,N- dimethylamino)benzyl)palmatine iodide . (Melting point: 153°C) Example 81: Preparation of 13-f2-fluoro-4-t-butylbenzyl)palmatine iodidefcomp. No. 81) 41 2-Fluoro-4-t-butylbenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.7 g of 13-f2-fluoro-4-t-butylbenzyl)palmatine iodide. (Melting point: 157 ° C) Example 82: Preparation of 13-f4-f2-hvdroxy-2-propyl)benzyl)palmatine iodidefcomp. No. 82) 4-(2-Hydroxy-2-propyl)benzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.6 g of 13-(4-(2-hydroxy-2-propyl)benzyl)palmatine iodide. (Melting point: 112 * C) Example 83: Preparation of 13-f 4- (2-methyl-2-butyl)benzyl)ρaImatine iodide fcomp. No. 83) 4-(2-Methyl-2-butyl)benzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.3 g of 13-(4-(2-methyl-2-butyl)benzyl)palmatine iodide. (Melting point: 110°C) Example 84: Preparation of 13-(4-methylthiobenzyl)palmatine iodidefcomp. No.84) 4-Methylthiobenzyl chloride, instead of 4-chlorobenzyl chloride of Example 67, was treated by the process described in Example 67 to give 2.7 g of 13-(4- methylthiobenzyl)palmatine iodide. (Melting point: 110°C) Example 85: Preparation of 2.3.9.10-tetraproρoxy protoberberine iodide (comp. No. 85) Berberine hydrochloride (2.5g) was reacted with 12.5g of anhydrous 42 aluminum chloride with stirring at 150°C for 1 hour, and 250ml of 5% dilute hydrochloric acid was added to the reaction mixture. The mixture was heated to dissolve thoroughly and then cooled to 4"C to precipitate a solid product. The solid thus obtained was filtered off and recrystallized from methanol to obtain 1.5g of 2.3,9, 10-tetrahydroxy protoberberine. To a solution of 5.0g of 2,3,9,10- tetrahydroxy protoberberine in 200 ml of acetonitrile was added 12.5g of potassium carbonate. The mixture was heated and 11.7ml of n-propyl iodide was added thereto dropwise under reflux for 5 hours. The reaction mixture was concentrated and then adsorbed with Celite and liquid-chromatographed over 60g of silica gel and eluted with methanol/dichloromethane(l:25) to obtain 2.87g of 2,3,9,10- tetrapropoxyprotoberberine iodide. NMR(300MHz, DMSO-d 6 ) 8: 1.10(m, 12H), 1.90(m, 6H), 2.00(m, 2H), 3.30(t, 2H), 4.10(m, 6H), 4.47(t, 2H), 5.15(t, 2H), 6.74(s, IH), 7.41(d, 2H), 7.70(d, 2H), 7.98(d, IH), 8.52(s, IH), 9.92(s, IH) Example 86: Preparation of 13-(4-t-butylbenzyl) berberine sulphatefcomp. No. 86) To a solution of5g of 13-(4-t-butylbenzyl)berberine chloride(compound No. 62) in 25ml of acetone was added dropwise 5g of 45% aqueous NaOH solution. The reaction mixture was stirred at room temperature and 20ml of distilled water was added thereto every two hours and after 5 hours, 30ml of distilled water was added thereto. The solid thus precipitated was filtered to obtain 5.1 g of 8- acetonyl-13-(4-t-butylbenzyl)berberine. To lg of 8-acetonyl-13-(4-t-butylbenzyl) berberine was added 4 ml of 1.0 M H 2 S0 4 . The reaction was proceeded at room temperature for 2 hours. The solid thus obtained is filtered and washed with small amount of distilled water and dried to obtain 0.8g of 13-(4-t-butylbenzyl)berberine sulphate. (Melting point: 152°C) Example 87: Preparation of 13-f4-t-butylbenzyl)berberine acetate fcomp. No. 87) 43 Acetic acid (3 ml), instead of 1.0 M H 2 S0 4 of Example 86, was treated by the process described in Example 86 to give 0.5g of 13-(4-t-butylbenzyl)berberine acetate. (Melting point: 171 "C) Example 88: Preparation of 13-(4-t-butylbenzyl)palmatine nitratefcomp. No. 88) 13-(4-t-Butylbenzyl) palmatine iodide obtained in Example 77, instead of 13- (4-t-butylbenzyl)berberine chloride of Example 86, was treated by the process described in Example 86 to obtain 8-acetonyl-13-(4-t-butylbenzyl)palmatine. Then, 1.3 M HN0 3 , instead of 1.0 M H 2 S0 4 of Example 57, was employed to obtain 0.8g of 13-(4-t-butylbenzyl)palmatine nitrate. (Melting point: 146°C) Example 89: Preparation of 13-benzyldihydroberberine fcomp. No. 89) To a vigorously stirred solution of 13-benzyl berberine iodide obtained in Example 1 in 50 ml of THF was added 5ml of 1.0M LiAIH 4 in a stream. After the reaction mixture was stirred for 1 hour, the reaction mixture was concentrated under vacuo and then extracted twice with 20ml of dichloromethane. The arganic extract was concentrated and then recrystallized from methanol to obtain 1.7g of 13-benzyldihydroberberine . NMR(300MHz, CDC1 3 ) 8: 2.60(m, 2H), 3.20(m, 2H), 3.66(d, IH), 3.85 (s, 2H), 3.87(s, 3H), 3.95(s, 3H), 4.27(d, IH), 5.99(s, 2H), 6.03(d, IH), 6.57(d, IH), 6.65(s, IH), 6.75(s, IH), 6.83-7.06(m, 5H) Example 90: Preparation of 13-f4-chlorobenzyl)dihydroberberinefcomp. No. 90) 13-(4-Chlorobenzyl) berberine iodide(2.0 g)obtained in Example 2, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.67 g of 13-(4-chlorobenzyl)dihydroberberine. 44 NMR(300MHz, CDC1 3 ) δ: 2.50(m, 2H), 3.21 (m, 2H), 3.66(d, IH), 3.86(s, 2H), 3.88(s, 3H), 3.99(s, 3H), 4.27(d, IH), 5.99(s, 2H), 6.05(d, IH), 6.63(d, IH), 6.70(s, IH), 6.75(d, 2H), 6.80(s, IH), 7.14(d, 2H) Example 91: Preparation of 13-(3-chlorobenzyl)dihvdroberberinefcomp. No. 91) 13-(3-Chlorobenzyl) berberine iodide(2.0 g) obtained in Example 3, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.61 g of 13-(3-chlorobenzyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.80-3.20(m, 4H), 3.60(d, IH), 3.75(s, 2H), 3.83(s, 3H), 3.88(s, 3H), 4.17(d, IH), 6.07(s, 2H), 6.13(d, IH), 6.60(d, IH), 6.65(s, IH), 7.05-7.15(m, 4H), 7.15(s, IH) Example 92: Preparation of 13-f2-chlorobenzyl)dihydroberberinefcomp. No. 92) 13-(2-Chlorobenzyl) berberine iodide(2.0 g) obtained in Example 2, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.50g of 13-(2-chlorobenzyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.80(m, 2H), 3.16(m, 2H), 3.57(d, IH), 3.73(s, 3H), 3.75(s, 2H), 3.83(s, 3H), 4.15(d, IH), 6.07(s, 2H), 6.14(d, IH), 6.70(d, IH), 6.73(s, IH), 6.86(d, IH), 7.01(t, IH), 7.10(t, IH), 7.12(s, IH), 7.21(d, IH) Example 93: Preparation of 13-f4-bromobenzyl)dihvdroberberinefcomp. No. 93) 13-(4-Bromobenzyl) berberine iodide(2.0 g) obtained in Example 5, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.61 g of 13-(4-bromobenzyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.50(m, 2H), 3.21(m, 2H), 3.66(d, IH), 3.86(s, 45 2H), 3.88(s, 2H), 3.99(s, 3H), 4.27(d, IH), 5.99(s, 2H), 6.05(d, IH), 6.63(d, IH), 6.70(s, IH), 6.75(d, 2H), 6.80(d, 2H), 7.14(d, 2H) Example 94: Preparation of 13-f3-bromobenzyl)dihvdroberberinefcomp. No. 94) 13-(3-Bromobenzyl) berberine iodide(2.0 g)obtained in Example 6, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.57 g of 13-(3-bromobenzyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.70(m, 2H), 3.20(m, 2H), 3.65 (d, IH), 3.70(s, 2H), 3.78(s, 2H), 3.80(s, 3H), 4.10(d, IH), 6.02(s, 2H), 6.13(d, IH), 6.62(d, IH), 6.70(s, IH), 6.90-7.05(m, 4H), 7.12(s, IH) Example 95: Preparation of 13-(4-methylbenzyl)dihvdroberberinefcomp. No. 95) 13-(4-Methylbenzyl) berberine chloride(2.0 g) obtained in Example 15, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.43 g of 13-(4-methylbenzyl)dihydroberberine. NMR(300MHz, CDC ) δ: 2.26(s ,3H), 2.57(m, 2H), 3.18(m, 2H), 3.66(d, IH), 3.77(s, 2H), 3.80(s, 3H), 3.89(s, 3H), 4.12(d, IH), 5.97(s, 2H), 6.21(d, IH), 6.67(d, IH), 6.71(s, IH), 6.75(d, 2H), 6.82(s, IH), 7.21(d, 2H) Example 96: Preparation of 13-f3-methylbenzyl)dihydroberberinefcomp. No. 96) 13-(3-Methylbenzyι)berberine chlride(2.0 g) obtained in Example 16, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.61 g of 13-(3-methylbenzyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.20(s ,3H), 2.80-3.20(m, 4H), 3.66(d, IH), 3.75 (s, 2H), 3.83(s, 3H), 3.88(s, 3H), 4.17(d, IH), 6.07(s, 2H), 6.13(d, IH), 6.60(d, IH), 6.65(s, IH), 7.05-7.15(m, 4H), 7.15(s, IH) 46 Example 97: Preparation of 13-f2-methylbenzyl)dihvdroberberinefcomp. No. 97) 13-(2-Methylbenzyl)berberine chloride(2.0 g)obtained in Example 17, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.67 g of 13-(2-methylbenzyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.27(s ,3H), 2.78(m, 2H), 3.17(m, 2H), 3.59(d, IH), 3.75(s, 2H), 3.78(s, 3H), 3.83(s, 3H), 4.16(d, IH), 6.01(s, 2H), 6.24(d, IH), 6.71(d, IH), 6.73(s, IH), 6.87(d, IH), 7.02(t, IH), 7.08(t, IH), 7.11(s, IH), 7.19(d, IH) Example 98: Preparation of 13-f3,4-dimethylbenzyl)dihvdroberberine(comp. No. 98} 13-(3,4-Dimethylbenzyl) berberine chloride(2.0 g) obtained in Example 18, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.53g of 13-(3,4-dimethylbenzyl) dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 2.21(s, 3H), 2.25(s ,3H), 2.59(m, 2H), 3.10(m, 2H), 3.67(d, IH), 3.80(s, 2H), 3.85(s, 3H), 3.91(s, 3H), 4.15(d, IH), 5.99(s, 2H), 6.25(d, IH), 6.69(d, IH), 6.73(s, IH), 6.80(d, IH), 7.01(d, IH), 7.22(d, IH), 7.27(s, IH) Example 99: Preparation of 13-(2.4-dimethylbenzyl)dihvdroberberine(comp. No. 99) 13-(2,4-Dimethylbenzyl) berberine chloride (2.0g) obtained in Example 19, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.47 g of 13-(2,4-dimethylbenzyl) dihydroberberine. 47 NMR(300MHz, CDC1 3 ) 8: 2.25 (s. 3H), 2.26(s ,3H), 2.60(m, 2H), 3.20(m, 2H), 3.65(d, IH), 3.79(s, 2H), 3.82(s, 3H), 3.89(s, 3H), 4.21(d, IH), 6.01(s, 2H), 6.17(d, IH), 6.75(d, IH), 6.82(d, IH), 6.02(d, IH), 7.10(d, IH), 7.14(s, IH), 7.25(d, IH) Example 100: Preparation of 13- 4-t-butylbenzyl)dihvdroberberine fcomp. No. 100) To a solution of 4-t-butylbenzyl chloride (1.20 g, 6.6 mmol) in 50ml of acetonitrile was added 0.998(6.6 mmol) of Nal. After the reaction was proceeded at room temperature for 1 hour, 2.02g(6.0mmol) of 8-hydroberberine was added to the reaction mixture. The reaction mixture was refluxed under N 2 atmosphere for 5 hours. The resulting mixture was concentrated and then dissolved in 100ml of chloroform and quenched with 10% aqueous solution of K 2 C0 3 The organic layer was separated and dried over MgS0 4 and filtered. The filtrate was concentrated. The resulting solid was triturated in 20ml of diethylether and filtered to obtain 2.60g of 13-(4-t-butylbenzyl)dihydroberberine NMR(300MHz, CDC1 3 ) δ: 1.31(s, 9H), 2.80-2.84(broad t, 7=5.7Hz, 2H), 3.15-3.19(broad t,7=5.7Hz, 2H), 3.81(s, 3H), 3.86(s, 3H), 4.10(s, 2H), 4.40(s, 2H), 5.89(s, 2H), 6.65(s, IH), 6.65-6.68(d, 7=8.4Hz, IH), 6.78-6.81(d, 7=8.4Hz, IH), 6.93(s, IH), 7.22-7.25 (d, 7= 8.1Hz, 2H), 7.30-7.32(d,7=8.1Hz, 2H) Example 101: Preparation of 13-(4-picolyl)dihvdroberberinefcomp. No. 101) 4-Picolylchloride(0.84g), instead of 4-t-butylbenzyl chloride of Example 100, is treated by the process described in Example 100 to obtain 2.01 g of 13-(4-picolyl) dihydroberberine . NMR(300MHz, DMSO-d 6 ) δ:2.73-2.77(broad t, 7=5.7Hz, 2H), 3.07-3.11(broadt,7=5.7Hz, 2H), 3.72(s, 3H), 3.73(s, 3H), 4.04(s, 2H), 4.34(s, 2H), 5.95(s, 2H), 6.56-6.58(d, 7=8.1Hz, IH), 6.58(s, IH), 6.74-6.76(d, 48 /= 8.1Hz, IH), 6.88(s, IH), 7.2S-7.29(d, 7=5.4Hz, 2H), 8.46-8.48(d, 7=5.4Hz, 2H) Example 102: Preparation of 13-f2-hydroxy-5-nitrobenzyl)dihydroberberinefcomp. No. 102) 2-Hydroxy-5-nitrobenzylbromide(1.53g), instead of 4-t-butyl benzyl chloride of Example 100, is treated by the process described in Example 100 to obtain 2.60g of 13-(2-hydroxy-5-nitrobenzyl)dihydroberberine. NMR(300MHz, DMSO-d 6 ) δ: 3.09-3.13(m, 4H), 3.34-3.38 (broad t, I=5.7Hz, 2H), 3.79(s, 3H), 3.83(s, 3H), 5.06(s, 2H), 6.14(s, IH), 6.23(s, IH), 6.84-6.87(d, I=8.7Hz, IH), 6.99-7.02(d, I=8.4Hz, IH), 7.07(s, IH), 7.15-7.18(d, I=8.4Hz, IH), 7.61-7.62(d, I=2.4Hz, IH), 7.71(s, IH), 7.93-7.96(dd, =2.4, 8.7Hz, IH), 11.38(s, IH) Example 103: Preparation of 13-benzvIhydropalmatine fcomp. No. 103) 13-Benzyl palmatine iodide (2.0 g) obtained in Example 66, instead of 13- benzyl berberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.75 g of 13-benzyldihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 2.60(m, 2H), 3.22(m, 2H), 3.67(d, IH), 3.87(s, 2H), 3.88(s, 3H), 3.94(s, 3H), 3.95(s, 3H), 3.96(s, 3H), 4.27(d, IH), 6.05(d, IH), 6.63(d, IH), 6.70(s, IH), 6.87(m, 3H), 6.92(m, 2H), 7.16(s, IH) Example 104: Preparation of 13-(4-chlorobenzyl)dihydropalmatine fcomp. No. 104) 13-(4-Chlorobenzyl)palmatine iodide(2.0 g) obtained in Example 67, instead of 13-benzyl berberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.61 g of 13-(4-chlorobenzyl)dihydropalmatine. 49 NMR(300MHz, CDC1 3 ) δ: 2.55 (m, 2H), 3.19(m, 2H), 3.65(d, IH), 3.80(s, 2H), 3.81(s, 3H), 3.90(s, 3H). 3.92fs, 6H), 4.25(d, IH), 6.05 (d, IH), 6.64(d, IH), 6.75 (s, IH), 6.84(d, 2H), 6.85(s, IH), 7.14(d, 2H) Example 105: Preparation of 13-f 3-chlorobenzyl)dihydropalmatine fcomp. No. 105) 13-(3-Chlorobenzyl)palmatine iodide(2.0g) obtained in Example 68, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.63 g of 13-(3-chlorobenzyl)dihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 2.80-3.10(m, 4H), 3.58(d, IH), 3.72(s, 2H), 3.83(s, 3H), 3.88(s, 3H), 3.90(s, 3H), 3.91(s, 3H), 4.21(d, IH), 6.10(d, IH), 6.67(d, IH), 6.69(s, IH), 7.05-7.15(m, 4H), 7.14(s, IH) Example 106: Preparation of 13-f2-chlorobenzyl)dihydropalmatine fcomp. No. 106) 13-(2-Chlorobenzyl)palmatine iodide(2.0 g) obtained in Example 69, instead of 13-benzylberberine iodide(2.0 g) of Example 89, is treated by the process described in Example 89 to obtain 1.57 g of 13-(2- chlorobenzyl)dihydropalmatine . NMR(300MHz, CDC1 3 ) δ: 2.65(m, 2H), 3.17(m, 2H), 3.60(d, IH), 3.71(s, 2H), 3.79(s, 3H), 3.85(broad s, 9H), 4.15(d, IH), 6.14(d, IH), 6.67(d, IH), 6.70(s, IH), 6.73(s, IH), 6.86(d, IH), 7.02(t, IH), 7.12(s, IH), 7.19(s, IH) Example 107: Preparation of 13-(4-bromobenzyI)dihydroρalmatine fcomp. No. 107) 13-(4-Bromobenzyl)ρalmatine iodide(2.0 g) obtained in Example 70, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.63 g of 13-(3-bromobenzyl)dihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 2.50(m, 2H), 3.25 (m, 2H), 3.64(d, IH), 3.85(s, 50 2H), 3.85(s, 3H), 3.90(s, 3H), 3.91(s, 3H), 3.93(s, 3H), 4.27(d, IH), 6.05(d, IH), 6.63(d, IH), 6.70(s, IH), 6.75(d, IH), 6.81(s, IH), 7.17(d, 2H) Example 108: Preparation of 13-f3-bromobenzyl)dihvdro palmatine fcomp. No.108) 13-(3-Bromobenzyl)palmatine iodide(2.0g) obtained in Example 71, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.62 g of 13-(3-bromobenzyl)dihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 2.70(m, 2H), 3.20(m, 2H), 3.65(d, IH), 3.70(s, 2H), 3.75(s, 3H), 3.80(s, 3H), 3.82(s, 3H), 3.83(s, 3H), 4.15(d, IH), 5.99(d, IH), 6.65(d, IH), 6.72(s, IH), 6.92(d, IH), 6.95(d, IH), 7.02-7.02(m, 2H), 7.12(s, IH) Example 109: Preparation of 13-(4-methylbenzyl)dihydropalmatine fcomp. No. 109) 13-(4-Methylbenzyl)palmatine iodide(2.0g) obtained in Example 72, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.59 g of 13-(4-methylbenzyl)dihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 2.25(s, 3H), 2.59(m, 2H), 3.27(m, 2H), 3.66(d, IH), 3.76(s, 2H), 3.81(s, 3H), 3.85(s, 3H), 3.87(s, 6H), 4.15(d, IH), 6.21(d, IH), 6.71(d, IH), 6.77(s, IH), 6.85(d, 2H), 6.92(s, IH), 7.11(d, 2H) Example 110: Preparation of 13-(3-methylbenzyl)dihvdro palmatine fcomp. No. 110) 13-(3-Methylbenzyl)palmatine iodide (2.0g)obtained in Example 73, instead of 13-benzyl berberine of Example 89, is treated by the process described in Example 89 to obtain 1.51g of 13-(3-methylbenzyl)dihydro palmatine. NMR(300MHz, CDC1 3 ) δ: 2.20(s, 3H), 2.79(m, 2H), 3.17(m, 2H), 3.59(d, 51 IH), 3.75 (s, 2H), 3.87(s, 3H), 3.90(s, 3H), 3.93(s, 6H), 4.17(d, IH), 6.13(d, IH), 6.59(d, IH), 6.67(s, IH), 7.04-7.10(m, 4H), 7.12(s, IH) Example 111: Preparation of 13-f2-methylbenzyl)dihvdro palmatine fcomp. No. ill) 13-(2-Methylbenzyl)palmatine iodide(2.0 g) obtained in Example 74, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.49 g of 13-(2-methylbenzyl)dihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 2.26(s, 3H), 2.79(m, 2H), 3.15(m, 2H), 3.59(d, IH), 3.75(s, 2H), 3.78(s, 3H), 3.83(s, 3H), 4.16(d, IH), 6.24(d, IH), 6.71(d, IH), 6.73(s, IH), 6.87(d, IH), 7.02(t, IH), 7.06(t, IH), 7.12(s, IH), 7.18(d, IH) Example 112: Preparation of 13-(3.4-dimethylbenzyl)dihvdro palmatine fcomp. No. 112) 13-(3,4-Dimethylbenzyl)palmatine iodide(2.0 g) obtained in Example 75, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.53 g of 13-(3,4-dimethylbenzyl)dihydro palmatine. NMR(300MHz, CDC1 3 ) δ: 2.17(s, 3H), 2.23(s, 3H), 2.59(m, 2H), 3.10(m, 2H), 3.65(d, IH), 3.80(s, 2H), 3.85 (s, 3H), 3.91(s, 3H), 3.93(s, 6H), 4.15(d, IH), 6.24(d, IH), 6.70(d, IH), 6.73(s, IH), 6.87(d, IH), 7.11(d, IH), 7.16(s, IH) Example 113: Preparation of 13-(2.4-dimethylbenzyl)dihvdropalmatine fcomp. No. 113) 13-(2,4-Dimethylbenzyl)palmatine iodide(2.0 g) obtained in Example 76, 52 instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.47 g of 13-(2,4-dimethylbenzyl)dihydro palmatine. NMR(300MHz, CDC1 3 ) δ: 2.20(s, 3H), 2.22(s, 3H), 2.65(m, 2H), 3.24(m, 2H), 3.65(d, IH), 3.79 s, 2H), 3.82(s, 3H), 3.87(s, 3H), 3.89(s, 3H), 3.91(s, 3H), 4.21(d, IH), 6.17(d, IH), 6.75(d, IH), 6.82(d, IH), 7.02(d, IH), 7.10(d, IH), 7.14(s, IH), 7.25 (d, IH) Example 114: Preparation of 13- 4-t-butylbenzyl)dihydro palmatine fcomp. No. 114) 13-(4-t-Butylbenzyl)palmatine iodide(2.0 g) obtained in Example 77, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.77 g of 13-(4-t-butylbenzyl)dihydropalmatine. NMR(300MHz, CDC1 3 ) δ: 1.22(s, 9H), 2.48(m, 2H), 3.33(m, 2H), 3.65(d, IH), 3.88(s, 2H), 3.85(s, 3H), 3.90(broad s, 6H), 3.97(s, 3H), 4.21(d, IH), 6.15(d, IH), 6.77(d, IH), 6.81(s, IH), 6.87(d, 2H), 7.05(s, IH), 7.19(d, 2H) Example 115: Preparation of 2.3,9, lO-tetrapropoxydihydroprotoberberine comp. No. 115) 2,3,9, 10-Tetrapropoxy protoberberine iodide(2.0 g) obtained in Example 78, instead of 13-benzylberberine iodide of Example 89, is treated by the process described in Example 89 to obtain 1.65 g of 2,3,9, 10-tetrapropoxydihydro protoberberine. NMR(300MHz, CDC1 3 ) δ: 1.10(m, 12H), 1.90(m, 6H), 2.00(m, 2H), 3.30(t, 2H), 3.90(broad s, 2H), 4.10(m, 6H), 4.47(t, 2H), 5.15(t, 2H), 6.00(s, IH), 6.31(d, IH), 6.79(d, IH), 6.79(s, IH), 6.85(s, IH), 7.16(s, IH) Example 116: Preparation of 8-fn-octyl)dihydroberberine fcomp. No. 116) 53 Mg(0.45 g) was added to 50ml of anhydrous ether and n-octyl bromide was slowly added thereto dropwise. The reaction was proceeded under reflux for 2 hours, and berberine chloride which had been dried completely was added thereto, and further refluxed for 2 hours. The resulting mixture was concentrated and dried, and then 50ml of water was added thereto. The solid thus precipitated was filtered off and dried, and then the agueous phase was extracted twice with 40ml of dichloromethane and concentrated to obtain 2.12g of 8-(n-octyl)dihydroberberine NMR(300MHz, CDC1 3 ) δ: 0.80(t, 3H), 1.10-1.40(m, 12H), 1.82(m, 2H), 2.65(m, 2H), 2.86(m, 2H), 3.76(s, 3H), 3.77(s, 3H), 3.87(s, 3H), 4.07(s, 3H), 5.86(s, IH), 6.64(d, IH), 6.76(s, IH), 6.83(d, IH), 7.22(s, IH) Example 117: Preparation of 8-f3-cyclohexylpropyl)dihydroberberine fcomp. No. 117) 3-Cyclohexylpropyl bromide, instead of n-octyl bromide of Example 116, was treated by the process described in Example 116 to obtain 2.07 g of 8-(3- cyclohexylpropyl)dihydroberberine. NMR(300MHz, CDC1 3 ) δ: 0.80(m, 2H), 0.90-1.20(m, 12H), 1.80(m, 4H), 2.75(m, 2H), 3.24(m, 2H), 3.66(s, 3H), 3.87(s, 3H), 5.92(s, IH), 6.00(s, 2H), 6.65(d, IH), 6.67(s, 2H), 6.93(d, IH), 7.27(s, IH) Example 118: Preparation of 8-fn-octyl)dihvdropalmatine fcomp. No. 118) Palmatine chloride, instead of berberine chloride of Example 116, is treated by the process described in Example 116 to obtain 2.15 g of 8-(n-octyl)dihydro palmatine. NMR(300MHz, DMSO-d 6 ) δ: 0.80(t, 3H), 1.10-1.40(m, 12H), 1.86(m, 2H), 2.75(m, 2H), 3.24(m, 2H), 3.72(s, 3H), 3.77(s, 3H), 4.58(broad s, IH), 5.85 (s, 2H), 6.05(s, 2H), 6.67(s, 2H), 6.93(s, IH) 54 Example 119: Preparation of 0.5% creamv formulation of comp. No. 62 Tefose 63(80 g) produced by GATTEFOSSE in France, 15.32g of Labrafil M 1944 CS produced by GATTEFOSSE in France and 14.4g of liquid paraffine were heated to 70°C and 2g of the compound No. 62 was added thereto and then suspended with stirring (8,000rpm) for 10 minutes. The suspension thus obtained was added to water solution at 70°C wherein 2.0g of disodium hydrogen phosphate (Na 2 HP0 ) was dissolved in 300g of purified water, and emulsified with stirring(8,000rpm) for 20 minutes. The emulsion thus obtained was cooled to 35°C with stirring and charged in tube by suitable amount. Example 120: Preparation of 0.5% creamy formulation of compound No. 85 Tefose 63(80 g) produced by GATTEFOSSE in France, 15.32g of Labrafil M 1944 CS and 14.4g of liquid paraffine were heated to 70°C and the compound No. 85 was added thereto and then suspended with stirring (8,000rpm) for 10 minutes. The suspension thus obtained was added to water solution at 70°C wherein 2.0g of disodium hydrogen phosphate (Na 2 HP0 4 ) was dissolved in 300g of purified water, and emulsified with stirring(8,000rpm) for 20 minutes. The emulsion thus obtained was cooled to 35°C and charged in tube by suitable amount. Example 121: Preparation of vaginal suppository of the compound No.62 The compound No. 62(10 g), 50g of succinic acid, lOOg of potassium sulphate, 20g of silicon dioxide(Si0 2 ) and 180g of lactose #100(100 Mesh) were mixed in mixer for 5 minutes, and 8,560g of lactose #100(100 Mesh) and l,000g of Ludipress produced by BASF in Germany were added thereto and then mixed for 10 minutes. Magnesium stearate(80 g) was added to the mixture and further mixed for 5 minutes. The resulting mixture was tableted using a punch to prepare 10,000 tablets of which thickness is 6.0mm and weight is l,000mg. (Hardness: 8KP, Friction loss: 0.2%, Disintegration rate: 120 seconds) 55 Example 122: Preparation of vaginal suppository of the compound No.85 The compound No. 85(10 g), 50g of succinic acid, lOOg of potassium sulphate, 20g of silicon dioxide(Si0 2 ) and 180g of lactose #100(100 Mesh) were mixed in mixer for 5 minutes, and 8,560g of lactose #100(100 Mesh) and l,000g of Ludipress were added thereto and then mixed for 10 minutes. Magnesium stearate(80 g) was added to the mixture and further mixed for 5 minutes. The resulting mixture was tableted using a punch to prepare 10,000 tablets of which thickness is 6.0mm and weight is l,000mg. (Hardness: 8KP, Friction loss: 0.2%, Disintegration rate: 110 seconds) Example 123: Antifungal efficacy of cream formulation against local fungal skin infection Specific pathogen free SKΗ/1 male mouse(Hairless) of which weight is 30- 35g and age is 5 weeks, was reared with sterilized water and feed for 12 night/day in the condition of 21-23°C and of 50% relative humidity. Five mice were allocated by each group. After skin infection, each mouse was taken in separate cage. The strain of microorganism for infection(Epidermophytonfloccosum) was cultivated on a flat medium of SDA(Sabouraud Dextrose Agar) for 5-7 days and after confirmation of macrocornidia, 3ml of RPMI (Rosewell Park Memorial Institute) 1640 media per each flat medium was added thereto and then scraped well using loop to remove hyphae from media. The floating liquid was suspended briefly and then diluted with RPMI 1640 media to adjust the concentration of hyphae to 2x10° CFU/ml. Mouse was anesthetized and marked on back site(lumbosacral area) in a shape of circle of which diameter is 1.5cm. Then, the inner part of marked skin was scratched with sand paper. The scratched part was covered with a filter paper to preserve inoculated microorganism for a long time and thereby stimulating the skin continuously. 0.2 ml of fungus solution of which concentration is adjusted as described above, was inoculated between the skin and filter paper. 5 Days after the inoculation, the filter paper was removed and the infection of skin was examined. The test formulations, 0.5% creamy formulations of the compounds Nos. 62 and 56 85, 1.0% creamy formulation of Terbinafme(Lamisil® cream) and a placebo was applied on the infected areas in a same amount once a day for 5 days. The clinical evaluation on the change of the infected area in 5 days after the inoculation was performed and expressed numerically from 0 to 4. The daily change of the infected area was checked every day. The result of each group was compared with each other. 0: Normal state 1: Mild erythema or small number of skin eruption 2: Well-demarcated erythema with scales or mild skin eruption of infected area 3: Wide area of marked skin eruption, scales, swelling or severe skin eruption with partial swelling and scales 4: The same as those of control, or severe skin eruption in entire lesion The result was calculated as follows; Efficacy(%) = 100- (Tx lOO÷K) T: Average score of clinical evaluation in drug treated area K: Average score of clinical evaluation in placebo control group Meanwhile, novel compounds having the above chemical formulae Nos. 1 and 2, and the relative activities thereof against Candida albicans (KCTC 1940), Aspergillus niger(ATCC 9642) and Saccharomyces cerevisiae in accordance with the agar dilution method in Sabouraud dextrose agar media, Czapek agar media and Yeast Extract-Peptone-Dextrose agar media, are described respectively in following Tables la-le and 2a-2c. The relative activities of novel compounds are evaluated and expressed as follows: the relative activity is 4 in case that the control drug, i.e., 57 Miconazole exhibits the fungistatic activity in agar media at a certain concentration; the relative activity of the novel compound is 4 in case that the novel compound exhibit the fungicidal activity at the concentration same as that of Miconazole; the relative activities of the novel compound are 3, 2, 1 respectively in case that the novel compound exhibits the fungicidal activity at 2, 4, 8 times higher concentration than that of Miconazole; the relative activities of the novel compound are 5, 6, 7 respectively in case that the novel compound exhibits the fungicidal activity at 1/2, 1/4, 1/8 times lower concentration than that of Miconazole. 53 Table la. Protoberbeπne salt derivative (chemical formula I) ccrr.o. 1 1 relative H . Rι. ' 1 K 1 Rs 1 activity -O-CHj-O- -H i -CCHs ! i ' -<*a-Q 1 2 -OCΞ3 I ' - H,-{ -C! 3 " ! 3 -a -CCH3 ! 3 1 Ci 4 -0-CHrO- -e -OCH3 I 3 i 1 Ci 5 -O-CH2-O-I -H -OCH3 I -απ,-Q ar 3 0 HX3Ϊ2-0- -H - CH3 I 2 ! Br 7 -o-carθ- ! -H -OCH3 Cl 2 1 ci α 8 -o-CHz-o- -tf -OCHs Cl 2 F 9 -0-CHrO- -H -OCH3 Cl 2 1 10 -0-CH 2 H>- -H -CCH3 Cl 2 F li -0-CHrO- -H -CCH3 Cl -^ ■ - p 2 12 ^XΑτ-0- -H -CCH3 Cl -^ - 2 13 -O-CHJ-O- -H -OCHa Cl 2 F F 59 Table lb. zcrπo. reiativa Nc. ' ? 1 A S ac vrcy 1 i i j ± " 3 ! "'" .i'j | Cl 2 ) — \_ j -5 — ^_~J _ i -r i ci 1 -C^- -CH, 1 -X2r - ! -a C - H,^ 3 CH, i 17 -0-CHrO- -a -CCΞ3 Cl 3 13 -0-CHrO- -a -CCH3 Cl 5 CH, is ^ Hj-O- -a -CCH3 Cl ' 3 H 3 C CH, 20 -0-CHrO- -a -CCH3 Cl 3 21 -0-CHrO- -a -OCH3 Cl -CHa- 2 C j 22 -0-CHrO- -a _/»» -QO Cl 2 -^ ^^ 23 --cvo- -H -CCH3 Cl 1 HjC NOj 24 -0-CHj-C- -tf -CCH3 Cl -°*0 1 25 -OCH O- -fl -CCHs Cl 2 1 c? 3 26 -o-^H O -fi -CCH3 Cl ^^C^ 3 3 27 -0-CHrO- i 1 -fl -CCH3 Cl -CHj-Q-OCH, 3 9/55704 60 Table lc. i ;cmp. ative ι 1 So. i i. < .13 ii j A , 0. rel activity 2$ -0- ΞrO- j -a -CCHa Cl i 3 CCHj i \ 2S 1 -c-cnro- ; -a -CGa Cl J 4 i i ; i CCHj CCH, I 30 -0-CHr < -a -CCHa Cl -CH,^{ ~ -.CHj 3 1 0,N -o-Cδz-o- -a -OCHs Cl ~ °^^D "CC? 3 4 32 -0- Hr - -a -CCHs , ci 3 CHj ) 33 - -CHrO- -a →XHa Cl 3 θ 2 34 -O-CHsrO- -a - CHg Cl -α HOø 3 35 -O-CSa-O- -a ^CHa Cl '■ ^OO 6 Tabie Id comp. n relative Rl. Ra F-3 r.4 A " 0 Nc. activity 2ό -VJ-'-.-.J—J- -CCH 3 ~- -cH ~* 3 3 CH r 37 -vJ-^.-ij-' - -H -CCH3 Cl -CH- 2 -^ →l-CHa 5 ' — H, .cn 3 r 38 -o-cvo- -H -CCΪ3 Cl 5 CH, CHI Q - 39 -Q-CHz-O- -H -0G.3 Cl C^-Q-→J Hi^C} 4 CH, _CH- r 40 -0- H Ϊ -O- -H -OCH3 Cl • CH j -N-fCH^CH, 4 CH, 41 -o-αvo- -H -CCH3 Cl -CH,- H-C-CHJ λ—^ CH, 3 F 62 Table le. i comp. i relative Rι. a R 3 ' R 4 A Rs no. ! I activity I i 42 -0-CHz-O- -H -0CH3 Cl -CH 2 - -C-CH 3 ! 3 0 2 N I /=\ H ^NH 43 -O-CH 2 -O- -H -0CH3 Cl - CH 2 N - 4 44 -O-CH2-O- -H -0CH3 Cl -c^-Q-H-^ 2 45 -O-CH 2 -O- -a -0CH3 Cl __ y OH 2 / =\ P^3 46 -O-CH 2 -O- -H -0CH3 Cl -CH 2 - -C-OH 2 ^ CH 3 =\ CH 3 47 -O-CH 2 -O- -H -0CH3 Cl -CH 2 . -OCH 2 -* CH 3 48 -O-CH 2 -O- -H -OCHa Cl -CHs-^H-CHCH^CH, 3 49 -0-CH 2 -0- -H -OCH3 Cl -CH 2 - -CHCH 2 CH 3 4 CH 3 9/55704 63 T blt relative | RL Ra R3 R 4 A Rs \0 activity 50 -3-CH 2 - - -4 -OCH3 Cl H 2 - →Q 4 1 1 -C I i 1 1 -CH 2 - ) 51 1 -O-CH2-C- -U ■ " 1 -OCK3 Cl _ VOH 3 i 1 if 0 1 52 -0-CH 2 -0- 3 I "H -OCH3 Cl -Ch j ^-O-Crt CH 2 CH, I 1 53 -O-Gv-0- -H -OCH3 Cl - Y_ OCH 2 Cri, 3 54 -O-CH 2 -Q- -H -OCH3 Cl -CH 2^ ~ ^SCH 3 2 55 -O-CH2-O- -H -OCH3 Cl -CH 2 ~ -SCH 2 CH 3 2 56 -0-CH2-0- ; -H -0CH3 Cl -CH, 57 , -O-CH2-O- j -K > -OCH 3 [ Cl -CH : ^ -CHCH 2 OH 64 Tabie la. • 3ia::\9 V.. 3 J " ^ ?4 4 - - 53 53 — ^"T/.-Z-.- - ' *y :ι -^ κ:> ^ 50 Cl 4 51 -0-CHΪ~> -a -CCH3 Cl -O-i^-^ -CCjCHj 52 Cl 7 65 Table li. άctivr -Λ-..-3 " \ --.I ~*-A-3 Cl * . * ~t "\Λ-.C3 ~ ^O Oti3 -^- -v rt3 -fl -CCH3 -CH r c; S3 - K 3 -q -CCH3 -o ' .^ t α -CCH. -3 -CCE3 70 - CS -CCEa ^ x "ST 71 -CCEa -fl — CH 'vA-ty » ST 72 -CCEa -CCK3 -α ^-Q- H_ 73 — CCH 3 - H, -fl -CCHa 5 74 -iXHc - CHj 75 -CCH3 -CCHj CH, laDie LI. ccmp. no. Ri. R: RJ ■ R4 A R5 ! relative activity " 6 -ocH 3 : -H ! -OCH3 '' I ! - 1r ! HjC i i /== CH 3 -~ -0CH3 j -H -0CH3 I 1 -CH 2 - — CH 3 , 6 i CH 3 I ! i 1 i 78 j -0CH3 -H j -0CH3 1 -CH 2 N j CH 3 ! 1 ! i /= \ CH 3 r 79 -OCH3 -H -0CH3 I -CH 2 //^N-CH, 4 1 ^— CH 3 H 3 1 " 80 -OCH3 -H -0CH3 I -CH 2 Q-N-CH 2 CH 3 4 CH 3 81 -OCH3 -H -0CH3 I -CH 2 :\ -C-CH 3 p ^ CH 3 /=\ CH 3 82 -OCH3 -H -OCH 3 I -CH 2 - -C-OH J X — / CH 3 83 -OCH3 -H -OCH3 I -CH 2 -^^-CHCH 2 CH 3 2 CH 3 84 -OCH3 -H -OCH 3 1 — CH 2 -^ ri— SCH 3 57 Table lj. relative R-. A 5 1 • ! activity 1 ~" u -CPr I -fl 5 i 3δ -C-CHr ~ v- -fl - .I3 BSOJ -CHj- -j-CH, 4 CH, 1 37 -OCH3 -CHj- -J-CH, 4 CH, 83 -OCH3 -fl -OCH3 HO3 ' -^ 7 _H-CH 3 3 CK j Table 2a. Protoberberine salt derivative(che ical formula II) comp. ! I relative No. | Ri. * ! R I I fe activity 39 j -0-CHrO- -CCH3 - a ! ~ ^^} ' 2 I 90 -O-CSr - | -H -CCHs -^ " "01 i 3 i 91 -0-CHs-O- -H -OCH3 3 cι ! 92 -0-CHr-O- -H -OCH3 -,-p 3 I j 33 -OCH2-O- -H -CCH3 - o v - 3 94 -CHar-O- -H -TCH. • 2 Br 95 O-&i-O- -H -0CH3 -C^^ ^-CH, 3 i 96 -0-CHrO- -H -coh 3 97 -0-CHrO -H -0CH3 - C H 2 - 3 98 -o-cHrθ- -fl -0CH3 3 99 -O-CH2-O- -H - CH3 3 100 -0-CHrO- -H - CHs 5 59 Table 2b. 1 r8'.at:ve N . xc 3C~V!tV id — ' CiZ~ * ^ — ~Xr.. 2 NC- ■ G2 -CCH3 2 HC 103 — *-C3 ^Λ-.73 -H -CC13 • 3 104 -CG-3 -OCE3 -H - Γ ΛV^V-C! 3 105 -XH3 -H -CCH3 2 106 -CCHj -CCr.3 -H -CCH3 2 α 1G7 -XIΪ 3 -CCH3 -a -OCH3 - o O* 2 108 - CHs -CCKs 2 3r 109 —CK3 -CCH3 -H -CCSs -CH J -Λ^ H J 3 110 -OCH3 -CCH3 -H -CCHs 2 CH, 111 -CCH 3 -H - CH3 2 -CHs^ -CH. 112 -CCH3 - CH3 -H -CCαs 3 CHj 113 -CCH3 -0CK3 -H -OCH3 K 3 C 114 -OCH3 0 -OCH3 -H HXHs CH, 115 -OPr -OPr -H OPr -fc * vo i aoi rs 12.Ϊ ivc Ccmp . No . L'ι R ?U ό ac: i i:y 115 -O-CHc-C- -v.sn: -CCK -ri 3 117 | -C- H2-O- -CH 2 -Cy-Hex -CCH3 3 118 -OCK3 -OCK3 -CέH-7 ~0Ch,3 3 The compounds of the above Table 1. are the first compounds which inhibit concurrently Chitin synthetase which take part in biosynthesis of chitin. i.e., the constituent component of the cell wall of fungus and sτerol 24-methyl transferase, one of the major enzymes for the distal biosynihetic pathway of Ergosterol which is the constituent component of the cell membrane. The MIC. data of the compounds Nos. 18, 29, 31, 35, 58, 60, 63, 64, 77 and 85, and the drugs for control, azole compound, i.e., Miconazole, Itraconazole and Amphotericiii B against Candida and Epidermophyton are described in the following Table 3a and 3b. Table 3a. MIC. data of the compounds IC( μ g/ml ) fungus comp. comp. comp. comp. comp. comp. comp. No.18 No. 29 No. 31 No. 35 No. 58 No. 60 No. 62 C. albicans ATCC 1023 6.25 6.25 C. albicans ATCC28838 3.125 3.125 0.78 1.56 3.125 ' 3.125 0.78 C. albicans ATCC11651 3.125 1.56 1.56 0.78 12.5 6.25 0.78 C. albicans KCTC 1940 6.25 1.56 1.56 0.78 12.5 6.25 0.78 C. albicans U. K. 12.5 6.25 C. albicans OY 019 6.25 6.25 1.56 1.56 12.5 12.5 0.78 C. albicans OY 003 6.25 6.25 l.co 0.78 6.25 6.25 0.78 C. albicans IFO 1385 12.5 25 3.125 6.25 25 25 6.25 C. parapsilosis > 100 >100 C. glabrata >100 > 100 C. glulliermendi 12.5 12.5 C. krusei 3.125 3.125 E. floccosum 12.5 12.5 72 Table 3b. MIC: p. g. ml ) rungus comp. comp. comp. comp. λlico- Itraco ¬ Amphots πo.53 no. 54 no. 77 no. 35 r.a oie nazole ricin 3 C. albicans ATCC 1023 5.25 5.25 5.25 1.56 C. albicans ATCC 25828 078 3.125 0.78 0.78 0.78 > 1C0 3.125 C. albicans ATCC 11651 0.78 3.125 0.78 0.4 3.125 > 1C0 1.56 C. albicans KCTC 1940 1.56 12.5 1.56 0.4 1.56 > 1C0 0.78 C. albicans U. K. 5.25 50 > 100 3.125 C. aibicans OY 019 1.56 0.4 1.56 0.4 1.56 > 100 0.4 C. albicans OY C03 6.25 6.25 0.78 0.4 1.56 >100 0.78 C. albicans EFO 1385 6.25 25 3.125 6.25 3.125 > 1C0 1.56 C. parapsilosis >100 12.5 3.125 3.125 C. glabrata > 100 >100 > 100 1.56 C. glulliermendi 12.5 > 100 3.125 1.56 C. krusei 1.56 3.125 0.78 3.125 E. floccosum 12.5 > 100 > 100 6.25 50 12.5 . Moreover, the antifungal efficacies of the 0.5% creamy formulation of the compounds Xos. 29. 35, 60, 62, 63 and 85, and 1% creamy formulation cf Terbmafine (Lamisil cream), which are measured by applying the above formulations on the skin infected with Epidermophyton floccosum of mouse which is the standard animal for the test of local fungal skin infection, i.e, Specific Pathogen Free SKH 1, are described in the following Table 4. Table 4. Antifungal efficacy against local fungal skin infection. 0 Efficacy (%) 0 day 1 day 2 day 3 day 4 day 5 day 6 day 7 day later later later later later later later later comp. no. 62 (0.5%) 0 40.0 55.4 69.4 72.6 76.3 79.2 85.4 5 comp. no. 63 (0.5%) 0 42.5 52.5 59.8 64.6 73.6 75.6 80.2 comp. no. 35 (0.5%) 0 36.0 57.9 68.4 72.2 74.0 75.4 76.8 comp. no. 85 (0.5%) 0 34.5 38.8 42.5 54.6 63.2 68.0 70.5 20 comp. no. 29 (0.5%) 0 30.0 33.4 34.6 48.0 56.4 63.0 65.8 comp. no. 60 (0.5%) 0 20.0 32.0 36.0 48.6 51.5 58.6 60.5 Terbinafine (1.0%) 0 40.0 57.9 68.4 70.2 71.4 73.4 75.7 Placebo 0 0 0 0 0 0 0 0 25. 30 74 The above 0.5% creamy formulation of the compound No. 62 was applied on infected area of 10 male patients with fungal infection of foot, in a proper amount once a day for five days. 8 Patients exhibit remedial effect more than 98%. Meanwhile, the toxicity test of the compound of the present invention was performed using mouse. The compound was suspended in propylene glycol. The resulting suspension was medicated respectively on 5 female rats and 5 male rats(SD) of which age are 5 weeks, via oral after 12 hour starvation. The general symtoms, weight change and lethal case of the above rats were investigated. In cases of tests of the compound Nos. 25, 62, 63 and 85 (delivery of 2,000mg/kg), the general symtom and weight change were normal and the lethal case was not observed. Moreover, the bacterial reverse mutation test (Ames test) using salmonella typhimurium, the chromosome aberration test using cultured lung cells derived from Chinese hamster and the micronucleous test using male ICR mice on the compounds Nos. 25, 62, 63 and 85 exhibit negative results without exception. The toxicity data on the compound Nos. 25, 62, 63 and 85 are described in the following Table 5. In light forgoings, the compounds of the present invention are safe in toxicity and have the excellent lethal effect and growth-inhibition effect against fungus. Table 5. The toxicity data on compounds Nos. 25. 62, 63 and 85 ac :ε coxier/ I generic toxicity on-, nc. I c rcmcsome , 1 deliver/ AMΞS i nucleus animal | sex Dso lacmormaiitv 1 rest | :est itest male >3,000 comp. no. 25 rats oral negaπve ne ative negative female >2,500 maie >3 / 7C0 comp. no. o2 rats oral negative negative negative * female >2 7C0 male >2,500 comp. no. 63 rats oral negative negative negative female >2,Q00 male >5,000 comp. no. 85 rats oral negative negative negative i female >5,0C0 The more pertinent important features of the present invention have been outlined above in order that the detailed description of the invention which follows will be better understood and that the present contribution to the art can be fully appreciated. Those skilled in the art can appreciate that the conception and the specific embodiment disclosed herein may be readily utilized as a basis for modifying or designing other structures for carrying out the same purposes of the present invention. Further, those skilled in the art can realize that such equivalent constructions do not depart from the spirit and scope of the invention as set forth in the claims.

Claims: / o ' " aat is claimed is: 1. A protoberberine salt derivative which is having the following chemical formula ι l) 0 ) wherein R 1 , R : , and R 4 may be the same or different, and represent -C 5 alkoxy, R 3 represents hydrogen or -C 10 alkyl, A represents inorganic acid ion, organic acid ion or halide, R' represents hydrogen, pyridylmethyl, substituted pyridylmethyl or a group having the following chemical formula(XT) (XI) wherein Z 1 , Z 2 , Z 3 , Z 4 and Z 5 may be the same or different, and independently of one another represent hydrogen, halogen, C x -C 5 alkyl, trifiuoromethyl, phenyl, substituted phenyl, nitro, G- alkoxy, trifhioromethoxy. hydroxy, phenoxy, vinyl, methoxycarboxyl groups. 77 2. The protoberberine salt derivative according to claim 1, wherein A " is a group selected from the group consisting of nitrate, sulfate, acetate, tartrate. maleate, succinate, citrate, fumarate, aspartate, salicylate, glycerate, ascorbate, ϋuoride, chloride, iodide or bromide. 3. The protoberberine salt derivative according to claim 1, wherein R 1 - R is methylenedioxy(-0-CH 2 -0-), R 3 is hydrogen, R 4 is methoxy, R 5 is 3,4- dimethylbenzyl and A " is chloride. 4. The protoberberine salt derivative according to claim 1, wherein R l - R 2 is methylenedioxy(-0-CH 2 -0-), R 3 is hydrogen, R 4 is methoxy, R 5 is A-(ten- butyl) benzyl and A ' is chloride. 5. The protoberberine salt derivative according to claim 1, wherein R 1 - R 2 is methylenedioxy(-0-CH 2 -0-), R 3 is hydrogen, R 4 is methoxy, R 5 is 4- pheny benzyl and A " is chloride. 6. The protoberberine salt derivative according to claim 1, wherein R 1 - R 2 is methylenedioxy(-0-CH 2 -0-), R 3 is hydrogen, R 4 is methoxy, R 5 is 4- isopropylbenzyl and A " is chloride. 7. The protoberberine salt derivative according to claim 1, wherein R 1 - R 2 is methylenedioxy(-0-CH 2 -0-), R 3 is hydrogen, R 4 is methoxy, R 5 is 4-(tert- butyl)benzyl and A is iodide. 8. The protoberberine salt derivative according to claim 1, wherein R 1 is propoxy, R 2 is propoxy, R 3 is hydrogene, R 4 is propoxy, R 5 is hydrogene and AJs iodide. 9. 7,8-Dihydroprotoberberine derivative and salt thereof of the following chemical formula (II) _ 2 (II) wherein R l , R 2 , and R 4 may be the same or different, and represent C x -C 5 alkoxy, R 3 represents hydrogen or - Q alkyl, R J represents hydrogen or a group having the following chemical formula (XI) z 2 z 1 \ / / - " \ z 3 - (XI) / \ Z° Wherein Z 1 , Z 2 , Z 3 , Z 4 and Z 3 may be the same or different, and independently of one another represent hydrogen, halogen, C : -C 5 alkyl, trifiuoromethyl, phenyl, substituted phenyl, nitro, C : -C 4 alkoxy, - alkylamino, acetyla ino, C x -C 3 trialkyl ammonium, guanidinyl, methylthio, ethylthio, trifluoromethoxy, hydroxy, phenoxy, vinyl, carboxyl and C1-C2 alkoxycarbonyl group. 10. The 7,8-dihydroprotoberberine derivative and salt thereof according to claim 9, wherein R r -R 2 is methylenedioxy(-0-CH 2 -0-), R 3 is octyl(-C 8 H 17 ), R 4 is methoxy and R J is hydrogen. 11. The 7,8-dihydroprotoberberine derivative and salt thereof according to claim 9, wherein R 1 is propoxy, R 2 is propoxy, R 3 is hydrogene, R 4 is propoxy. 79 R 5 is hvdrcsene. 12. .An antifungal formulation containing protoberberine salts derivatives of the following chemical formula (I), and pharmaceutically allowable excipient or vehicle. (I) wherein R 1 , R\ and R 4 may be the same or different, and represent -C 5 alkoxy, R 3 represents hydrogen or C x -C w alkyl, A " represents inorganic acid ion, organic acid ion or halide, R 5 represents hydrogen pyridylmethyl, substituted pyridylmethyl or a group having the following chemical formula(XI) (XI) wherein Z 1 , Z 2 , Z 3 , Z 4 and Z 2 may be the same or different, and independently of one another represent hydrogen, halogen, C C 5 alkyl, trifiuoromethyl, phenyl, substituted phenyl, nitro, - alkoxy, C^-C^ alkylamino, acetylamino, C r C s trialkyl 30 ammonium, guanidinyl. methylthio. ethylthio, trifluoromethoxy. hydroxy, phenoxy, vinyl, carboxyl and C1-C2 alkoxycarbonyl group. 13. An antifungal formulation containing 7,8-dihydroprotoberberine salts derivatives of the following chemical formula (II), and pharmaceutically allowable excipient or vehicle. (II) wherein R 1 , R 2 , and R 4 may be the same or different, and represent C r C 5 alkoxy, R 3 represents hydrogen or C C 10 alkyl, R 3 represents hydrogen, pyridylmethyl, substituted pyridylmethyl or a group having the following chemical formula (XI) (XI) wherein Z\ Z 2 , Z 3 , Z 4 and Z 5 may be the same or different, and independently of one another represent hydrogen, halogen, C x -C 5 alkyl, trifiuoromethyl, phenyl, substituted phenyl, nitro, C^-C alkoxy, - alkylamino, acεtylamino, C r C s trialkyl ammonium, guanidinyl, methylthio, ethylthio, trifluoromethoxy, hydroxy, phenoxy, 8 1 vinyl, carboxyl and C-C z alkoxycarbonyi group. 14. A Process for preparing 13-substituted berberine salt of the following chemical formula (V), in which: berberine salt of the following chemical formulae (III) is reacted with NaBH and potasium carbonate to prepare dihydroberberine of the following chemical formula (VI); the compound thus obtained is reacted with alkyl substituent (R 5 -X) to prepare 13-alkyldihydroberberine compound of the following chemical formula (VII): and the compound thus obtained is oxidized with N-chlorosuccinimide(NCS). 3 (III) OCH 3 (VI) (VII) 32 3 (V) wherein R 1 and R 2 may be the same or different, and represent C -C 5 alkoxy, X " represents inorganic acid ion, organic acid ion or halide, R° represents hydrogen, pyridylmethyl, substituted pyridylmethyl or a group having the following chemical formula (XI) z 2 Z ' \ -— — / r " \ (XI) λ \\\ ■ c H , ' ' \ - z ~ Z 3 wherein Z 1 , Z 2 , Z 3 , Z 4 and Z 5 may be the same or different, and represent hydrogen, halogen, -C 5 alkyl, trifiuoromethyl, phenyl, substituted phenyl, nitro, -Q alkoxy, trifluoromethoxy, hydroxy, phenoxy, vinyl, methoxycarbonyl groups. 15. The process for preparing 13-substituted berberine salt according to Qaim 14, wherein 1.0 mol of berberine salt of the chemical formula (III) is reacted with 1.0 to 3.0 mol of NaBH and 2.0 to 4.0 mol. of potassium carbonate in alcohol solvent to prepare dihydroberberine of the chemical formula (VI). 16. The process for preparing 13-substituted berberine salt according to Qaim 14, wherein dfhydroberberine the chemical formula (VI) is reacted with 1.0 to 3.0 mol of alkyl substituent (R 5 -X) in organic solvent to prepare 13- alkyldihydroberberine compound of the chemical formula (VII). 1 " . A Process for preparing 13-substituted palmatine salt of the chemical formula (V), in which: palmatine salt cf the chemical formula (III) is reacted with NaBH and potassium carbonate to prepare dihydropalmatine of the chemical formula (VI); the compound thus obtained is reacted with alkyl substituent (R 5 -X) to prepare 13-alkyldihydropalmatine of the following chemical formula (VII): and the compound thus obtained is oxidized with N-bromosuccinimide(XBS). (III) 3 (VI) (VII) (V) CH wherein R 1 and R 2 may be the same or different, and represent C C ; alkoxy, X " represents inorganic acid ion, organic acid ion or halide, R J represents hydrogen, pyridylmethyl. substituted pyridylmethyl or a group having the following chemical 34 formula (XI) z 2 z Z 3 / (XI) Z 5 wherein Z 1 , Z 2 , Z 3 , Z 4 and Z 5 may be the same or different, and represent hydrogen, halogen, C x -C 5 alkyl, trifiuoromethyl, phenyl, substituted phenyl, nitro, -C, alkoxy, trifluoromethoxy, hydroxy, phenoxy, vinyl, methoxvcarbonyl groups. 18. The process for preparing 13-substituted palmatine salt of the chemical formula (V) according to Qaim 17, wherein 1.0 mol of palmatine salt of the chemical formula (III) is reacted with 1.0 to 3.0 mol of NaBH 4 and 2.0 to 4.0 mol of potassium carbonate in alcohol solvent to prepare dihydropaimatine of the chemical formula (VI). 19. The process for preparing 13-substituted palmatine salt of the chemical formula (V) according to Qaim 17, wherein dihydropaimatine of the chemical formula (VI) is reacted with 1.0 to 3.0 mol. of alkyl substituent (R 5 -X) in organic solvent to prepare 13-alkyldihydroberberine compound of the chemical formula (VII).

Inventors: JHONG TAE NEUNG, KIM EUI DEOK, KIM JUNG HO, KIM SEUNG UN, LEE YOU SUK, PAIK YOUNG KI, PARK JOON SEO, JHONG, TAE, NEUNG, KIM, EUI, DEOK, KIM, JUNG, HO, KIM, SEUNG, UN, LEE, YOU, SUK, PAIK, YOUNG, KI, PARK, JOON, SEO
Applicants: HANWHA CORP, HANWHA CORPORATION
Application year: 1998
Main classification: C07D455
IPC codes: A61K31, A61K36, A61P31, C07D455, C07D471, C07D491