ENANTIOSELECTIVE EPOXIDE HYDROLASES AND GENES ENCODING THESE

Abstract: The invention is directed to an isolated microorganism capable of selectively degrading epichlorohydrin or related halopropanol compounds, said microorganism being a representative of Agrobacterium spp and comprising a nucleic acid molecule encoding a polypeptide having enantioselective epoxide hydrolase activity.

Description: Title: Enantioselective epoxide hydrolases and genes encoding these. The present invention relates to (microbial) epoxide hydrolases and biocatalytic reactions yielding optically pure epoxides and diols. Optically active epoxides and 1,2-diols are important building blocks for the production of a range of optically active compounds that are needed for the production of pharmaceuticals and other fine chemicals. A number of strategies are available for the production of optically active epoxides (Schurig et al. 1992). An example that involves a catalytic asymmetric reaction is the Katsuki- Sharpless oxidation of unactivated double bonds, by means of an optically active titanium tartrate complex. A limitation is that the double bond must be in the allylic position relative to the hydroxyl group; this is necessary for coordination of the catalyst. Other procedures for the epoxidation of unfunctionalized prochiral alkenes have been developed using metalated salen- and porphyrin complexes as catalysts (Schurig et.al. 1992). These methods have a low selectivity for terminal epoxides and limited applicability due to high catalyst cost and low turnover numbers. In general, the above methods cannot be applied economically on a large scale. Biocatalytic reactions yielding optically pure epoxides have been described (Beetham et al. 1993; Faber et al, 1996) . Lipases can be used for the resolution of racemic esters of epoxyalcohols to produce both enantiomers of the epoxyalcohol . Similarly, lipases may be used for the stereoselective resolution of esters formed from haloalcohols and a carboxylic acid. By trans- esterification, enantiomerically pure alcohols can be obtained, which may be converted to epoxides. Biocatalytic production of epoxides is also possible by using mono-oxygenases . These enzymes require an unsaturated substrate and molecular oxygen. Reducing cosubstrates are required, and in general whole cells must be used to make regeneration of the cofactor economically feasible. Haloperoxidase based routes are also known. Haloperoxidases may produce optically active haloalcohols from alkenes. Enzymatic kinetic resolution is a possible technique for the production of optically active epoxides. Enzymes that hydrolyse epoxides are called epoxide hydrolases. They convert epoxides to diols by cleaving the epoxide ring with water. Epoxide hydrolases have been detected in mammals, insects, yeasts, fungi, other eukaryotic organisms, and in prokaryotic organisms. The epoxide hydrolases from higher eukaryotic organisms play a role in endogeneous metabolism and do not show strong substrate selectivity. They convert a variety of compounds formed from drugs by oxidative enzymes such as cytochrome P450. The sequence similarity of some epoxide hydrolases to dehalogenases (Beetham et al. 1995, Arand et al. 1994) suggests that they may act by covalent catalysis via a mechanism that does not allow racemisation (Lacourciere et al.,1993; Pries et al., 1994) . . The epoxide ring is opened by a nucleophilic attack of an carboxylate residue, yielding a covalent ester intermediate. Subsequently this intermediate is hydrolysed and the diol is released. Production of epoxide hydrolases by eukaryotic organisms is well known. Most work has been done with crude microsomal preparations of liver tissue from rats or other mammals (Wistuba et al. 1992). Mammalian epoxide hydrolases have been studied in detail because epoxides are important in toxicology and in chemical carcinogenesis (Guengerich, 1982) . However, the enzymes from mammals, which are known to be stereoselective in some cases, are difficult to obtain and serve multiple functions in vivo. Styrene oxides are well investigated substrates for microsomal epoxide hydrolases. They have been used in a standard assay for determinaton of the activity of the enzyme. Styrene oxides with substituents on the ring were investigated for studies on the mechanism of this enzyme (Dansette et al . 1978). Further research showed that the microsomal epoxide hydrolase was able to hydrolyse enantioselectivily styrene oxide ( atabe et al . 1983). The time course of this enzymatic hydrolysis showed a biphasic shape. At first the (R) -enantiomer is hydrolysed. After 90% conversion of the (R) -enantiomer, the (S) -enantiomer is hydrolysed at a much faster rate. This behaviour was explained by the fact that the (R) -enantiomer with a smaller K m could inhibit the hydrolysis of the faster reacting (S) -enantiomer (higher K m , higher V max ) . The enantioselective hydrolysis with mEH was also investigated with p-nitro styrene oxide (Westkaemper et al. 1980) and β- alkyl substituted styrene oxides (Belluci et al 1993 and 1996) . Fungal or yeast enzymes are also known and used experimentally. These also are likely to serve detoxification functions or are involved in endogeneous metabolism and are also not very selective. Recently, epoxide hydrolases from Aspergillus niger have been used to resolve successfully styrene oxide (Chen et al. 1993) and para substituted styrene oxides such as p-nitro styrene oxide and p-chloro styrene oxide with high enantioselectivity towards the S-enantiomer (Nelliah et al . 1996, Pedragosa-Moreau et al 1996) . Another fungus, Beauveria sulfurescens, showed high R-selectivity for styrene oxide. However, production in prokaryotic expression systems of substantial amounts of the epoxide hydrolases that originate from eukaryotes has shown not to be practically feasible. Although many of the above enzymes can be studied experimentally, none of them is available for industrial biocatalytic application. Typical industrial processes which would benefit, however, from the availability of well defined epoxide hydrolases would be the preparation of enantiopure epoxides and 1,2-diols as for example is useful in the production of pheromones and vitamins. Bacterial epoxide hydrolases have been detected in epichlorohydrin- degraders (Nakamura et al. 1994, Jacobs et al . 1991), in organisms that degrade epoxysuccinic acid (Hand et al. 1969) , and in organisms that convert nitrils (Hechtberger et al. 1993) . The bacterial epoxide hydrolases probably serve a function in the metabolism of endogenous compounds or epoxides produced by monooxygenases, and their selectivitity is not considered high. In a recent review on microbial epoxide hydrolases (Faber et al, 1996) it was observed that epoxide hydrolases could amply been found in eukaryotic cells but were rare in prokayotic cells. However, in some isolates from bacterial genera such as Coryr.ej acteriu-τι, Pseudomonas , Ba cillus , Mycoba cterium and Rhodococcus epoxide hydrolase activity has been observed. Faber et al also described that epoxide hydrolase activity could be induced in only a limited number of bacterial isolates, notably in Rhodococcus spp and in Mycoba cterium , whereas isolates from Coryneba cterium and Pseudomonas spp did not show any epoxide hydrolase activity and epoxide hydrolase could not be induced by growing these latter species on a selective medium. In addition, Faber et al remark that, although prokaryotic epoxide hydrolases have some advantages above eukaryotic epoxide hydrolases, the enantioselectivities of microbial hydrolases (expressed as enantiomeric ratio (ee) ) are low and highly substrate dependent. Faber further remarks, that only a few microbial strains possessing suitable epoxide hydrolase activity for a given substrate are known and prediction of suitable microbial strains is not yet possible, until, for example, the three-dimensional X-ray structure of an epoxide hydrolase has been solved. The present invention now surprisingly provides isolated micro-organisms that express epoxide hydrolases with a high enantioselectivity. Said micro-organisms represented and provided for by the invention can selectively degrade epichlorohydrin or related halopropanol compounds and their genome encodes a polypeptide having a highly enantioselective epoxide hydrolase activity. The isolated micro-organism which is the representative micro-organism provided by the invention is Agrobacterium spp exhibiting high enantioselectivity. The micro-organisms provided by the invention essentially correspond to the micro-organism represented by Agrobacterium radiobacter deposited under deposit number CBS 750.97. The terms "essentially correspond" or "essentially corresponding" refer to variations that occur in nature and to artificial variations of representative micro-organisms which can selectively degrade epichlorohydrin or related halopropanol compounds and are having enantioselective epoxide hydrolase activity. In particular said variations relate to variations in the epoxide hydrolase and functional fragments thereof which can be isolated from said micro-organisms and to variations in the genome of said organisms encoding epoxide hydrolase and functional fragments thereof. In particular, the terms "essentially correspond" or "essentially corresponding" relate to those variations that still allow detection of epoxide hydrolase or functional fragments thereof in tests for epoxide hydrolase activity or detection of the genome (be it DNA or RNA) or fragments thereof encoding epoxide hydrolase or functional fragments thereof by hybridization techniques, (such as nucleic acid blotting or in si tu hybridization) or amplification techniques (such as PCR) . The invention also provides a pure culture of the micro-organism, and a (lyophilized) preparation thereof. The invention also provides a crude or pure enzyme preparation derived from said micro-organism, preparing and purifying enzymes from micro-organisms comprise ordinary skills known in the art. In addition, the invention provides (partly) purified epoxide hydrolase. The enzyme provided by the invention has a molecular weight of 30-40 kD as determined by SDS-polyacrylamide electrophoresis and contains 280-350 amino acids. Epoxide hydrolase as provided by the invention is generally water soluble, generally stable in Tris buffer at neutral pH and is generally able to convert epoxides with a broad specificity. It catalyses the conversion by covalent catalysis, using a nucleophilic aspartate. The invention now also provides the isolated gene (a recombinant DNA molecule) or parts thereof encoding at least a functional part of a polypeptide having epoxide hydrolase activity. Such genes or fragments thereof can for example be derived from micro-organisms essentially corresponding to those that can selectively degrade epichlorohydrin or related halopropanol compounds. Furthermore, the invention provides a recombinant DNA molecule encoding a polypeptide exhibiting enantioselective epoxide hydrolase activity. In figure 1, the coding nucleic acid sequence of the gene encoding the epoxide hydrolase of Agrobacterium radiobacter (deposit number CBS 750.97) is shown. Furthermore, the invention provides genetically modified epoxide hydrolases with altered epoxide hydrolase activity. Modification is now possible because the functional catalytic site is known. The catalytic site comprises at least the amino acid residues at or around positions 107, 246 and/or 275 of the sequence shown in figure 1. The invention provides genomically related epoxide hydrolase in which at least one codon is mutated to encode for an amino acid providing the gene product with a modified epoxide hydrolase activity. Modification is now also possible because the invention provides host cells (such as bacteria or yeast cells, but many host cells are known in the field) that can be transformed via recombinant technology to express epoxide hydrolases and modified versions thereof, and functional fragments thereof. Such host cells can for instance be transformed with a vector comprising a recombinant DNA molecule encoding epoxide hydrolase activity derived from representative microorganisms that can selectively degrade epichlorohydrin or related halopropanol compounds and which genome encodes a polypeptide having a highly enantioselective epoxide hydrolase activity. Now that epoxide hydrolase can be produced via recombinant DNA technology, it is possible to obtain it in large quantities in a pure form. This enables for the first time crystallisation of epoxide hydrolase and resolution of its three-dimensional structure, allowing further prediction of microbial strains possessing suitable epoxide hydrolase activity for a given substrate, and allowing further selection of epoxide hydrolases with various selective specificities. The invention also provides a method of stereoselectively converting racemic mixtures of optically active epoxides to diols, using an epoxide hydrolase obtained from a micro-organism or host cell provided by the invention. The intermediate- and end-products of this conversion are enantiomerically pure epoxides and/or enantiomerically pure diols that can easily be extracted from the reaction mixture by suitable extraction methods known in the art. In said method the reaction can be performed by a pure or semi-pure culture of the microorganism or by a preparation thereof, or by a crude or pure enzyme preparation derived from said micro-organism or host cell, making industrial application of epoxide hydrolases as biocatalysts in a variety of reactions possible. Immobilized or free enzyme may be used. For immobilisation a wide range of techniques can be applied, for example the absorption of the isolated enzyme or the whole cells onto a water-insoluble macroscopic carrier. Some examples of carriers that can be used are Celite, cellulose, or ion exchange resins such as DEAE-cellulose or DEAE-Sephadex. Another technique is the covalent attachment on an macroscopic carrier such as porous glas or dextran. The isolated enzyme molecules can be crosslinked to each other using a crosslink molecule such as glutardialdehyde . The enzymes can also be entrapped into reversed micelles using an organic solvent and a detergent such as Aerosol OT . The epoxide hydrolase activity of epichlorohydrin- degrading organisms can be used for the kinetic resolution of mixtures of stereo-isomers of various epoxides, such as styrene oxides or other epoxides containing an aromatic group. These epoxides are represented by the formula given in figure 2. An epoxide is allowed to react in the presence of the epoxide hydrolase. After a certain amount is converted, the reaction is stopped and the product is isolated. The present invention is accordingly also embodied by the process for preparing enantiomerically enriched epoxides and/or diols, comprising contacting a racemic epoxide of the formula wherein Rl and R2 are independently selected from the group of hydrogen and organic radicals with an isolated microorganism capable of selectively degrading epichlorohydrin or related halopropanol compounds, said micro-organism being a representative of Agrobacterium spp and comprising a nucleic acid molecule encoding a polypeptide having enantioselective epoxide hydrolase activity. Rl and R2 are preferably selected from the group of hydrogen and, optionally substituted, straight or branched alkyl, alkenyl, aryl, aralkyl, alkaryl groups. An enzyme preparation is obtained from the epichlorohydrin-degrading micro-organism Agrobacterium radiobacter or from a recombinant bacterium that expresses an epoxide hydrolase gene obtained from an epichlorohydrin- degrading micro-organism. The epoxide hydrolase gene was cloned by means of the polymerase chain reaction. Based on the N-terminal and C-terminal amino acid sequence, two degenerate primers were designed. Chromosomal DNA was amplified and the resulting 900 bp PCR product was translationally fused in the Ncol- (startcodon) -site of pGEF+, resulting in pEH20. The gene is under control of the strong T7 promotor and typically 100-200 mg of purified epoxide hydrolase can be obtained from one liter of culture of E. coli BL21. The fl origin of replication makes it a suitable mutagenesis and sequence vector. The epoxide hydrolase is produced by growing a host cell in a fermentor under conditions which cause expression. Both constitutive production or induced production of the enzyme is possible. Alternatively, the DNA encoding for the epoxide hydrolase of an epichlorohydrin degrading bacterium is cloned into an expression vector by PCR amplification and cloning of the amplified DNA or by cloning of restriction enzyme fragments. The cloned DNA is expressed by induction or by stimulating expression of a specific polymerase. Subsequently, the epoxide hydrolase is produced under standard conditions. The epoxide hydrolase is remarkably similar to haloalkane dehalogenase from Xaπt ojbacter autotrop icus GJ10, both in structure and reaction mechanism. Based on homology, we classify this enzyme as an α/β hydrolase fold enzyme. The catalytic triad residues (Asp 107 -His 275 -Asp 246 ) are identified by sequence similarity and by mutation. Further it is shown that the reaction mechanisms of epoxide hydrolase proceeds via a covalently bound ester intermediate. The three dimensional structures of bromoperoxidase A2 and haloalkane dehalogenase are solved and their structural elements are displayed in the sequence alignment in figure 3.. Also the consensus of four secondary structure prediction programs for epoxide hydrolase is shown. Clearly, the predicted structural elements of the main domain coincide. The sequence alignment shows that the catalytic residues of bromoperoxidase A2 and haloalkane dehalogenase are also conserved in epoxide hydrolase (marked by D and H) The plot in figure 4 shows the topology of the α/β- hydrolase fold of bromoperoxidase A2 and haloalkane dehalogenase. The main domain consists out of a central β- sheet surrounded by α-helices. The cap domain is formed out of α-helices and is specific for each enzyme. The catalytic triad residues, the nucleophile (D107) , histidine (H275) , and the acid residue (D246) occupy preserved positions in the topology. Sequence similarity studies pointed out the residues Aspl07, His275, and Asp246 as the catalytic residues of epoxide hydrolase, i.e. the functional part thereof. Mutants were constructed to support this hypothesis. All mutant enzymes were expressed in crude extract as soluble enzyme and in quantities comparable to wild type epoxide hydrolase. Steady state activities were determined in crude extract. Aspl07Ala, Aspl07Glu, His275Arg and His275Glu mutants were not catalytically active, only the Asp246Ala mutant had residual activity left. Single turnover experiments with the mutant His275Arg enzyme and epichlorohydrin which were quenched after different reaction times showed that epichlorohydrin disappeared within seconds without the formation of product. We conclude that the diol formed from epichlorohydrin is covalently bound to the enzyme. The reaction that we postulate but that in no way can be seen as limiting the invention for epoxide hydrolase is as follows. Asp 107 performs a nucleophilic attack on the primary carbon atom of the epoxide ring. Incorporation of 18 0 labelled water occurs only at the primary carbon atom. A water molecule -that' is activated by His 275 attacks the carbonyl function of Asp 107 , and product is released. Asp 246 has a charge relay function. In analogy with Trp 125 in haloalkane dehalogenase, Phe 108 could be involved in substrate binding. We also expect the presence of a proton donor group, located in the cap domain. In conclusion, the epoxide hydrolase of Agrrobacterium spp. belongs to the class of α/β-hydrolase folded enzymes. The displayed sequence similarity, the structural features (secondary structure prediction and circular dichroism) , the inactive active site mutants, and the existence of an ester intermediate are all in agreement . It is now possible to study the kinetics of epoxide hydrolase by performing rapid quench and stopped flow experiments. Stopped flow experiments are possible since substrate is able to quench the fluorescence signal of some of the tryptophans present in epoxide hydrolase . Also, it is possible to mutate (replace) the amino acids (e.g. tryptophans) that are involved in substrate binding. Either with site-directed mutagenesis or random mutagenesis, it is possible to modify the substrate specificity and the stereoselectivity of epoxide hydrolase. The epoxide hydrolase has the following characteristics. * 34 kDa monomeric enzyme (approximately 294 amino acids) • high expression in E. coli and stable at 4°C for at least half a year • epoxide hydrolase contains considerable amounts of α- helical and β-stranded structure as is demonstrated by Circular Dichroism • epichlorohydrin is a suitable substrate (V max = 25 μmol min "1 .mg. protein "1 , K_, < 30 μM) Its DΝA sequence is shown in figure 1. It is generally soluble, generally stable in Tris buffer at neutral pH and is able to convert several epoxides with a broad specificity. The epoxide hydrolase is isolated and used directly or stored as a lyophilised preparation. For lyophilization, the enzyme is dialysed against a suitable buffer. Immobilised or free enzyme may be used. The asymmetric hydrolysis of the substrate is generally conducted in aqueous solution. The pH of the solution is maintained by means of a buffer. Maximum enzyme activity is obtained at pH 9 but a range of pH 5 to 11 can be employed. The composition of the buffer can for example be Tris/H 2 S0 4 , potassium phosphate or glycine/ΝaOH. The maximum enzyme activity is obtained at a temperature of 50°C, but the reaction can be conducted at a temperature ranging from 5°C to 65°C. The substrate concentration used to measure the enzyme activity is from 1 to 20 mM. In case of poorly soluble substrates, the water solubility can be increased by adding a miscible organic cosolvent such a DMSO. Also other organic solvents can be used. Generally a higher cosolvent concentration increases the maximum solubility of the substrate, but it must not be so high as to inactivate the enzyme. The epoxide can also be introduced as a second phase. This can either be done by dissolving the epoxide in a non-miscible organic solvent such as octane or the epoxide itself can be added, as a liquid or a solid. This is then brought into contact with an aqueous phase containing the enzyme and the two phases are subsequently vigorously mixed. After completion of the reaction, the epoxide can easily be recovered or obtained from the organic solvent. In a typical experiment, the substrate is dissolved in a quantity of buffer, with or without cosolvent, to a concentration of 1 mM to 250 mM. The lyophilised enzyme preparation is dissolved in a small quantity of buffer and is allowed to stabilise for some time. The enzyme solution is subsequently mixed with the buffer solution. The reaction is monitored by analysis of the degree of conversion of the epoxide or by appearance of the product of the conversion, the diol. This is determined by sampling a part of the reaction liquid and extracting it with an appropriate organic solvent. For the extraction of the epoxides diethyl ether can for example be used and for the diol ethylacetate can for example be used. The enantiomeric composition of the epoxides is determined by a gaschromatography using a chiral column such as CP-cyclodextrin-b2, 3, 6-M19. Using the epoxide hydrolase, a large number of epoxides containing an aromatic group can be isolated in enantiomerically pure form by degrading the unwanted enantiomer. This can be done for e.g. styrene oxide, p-methylstyrene oxide, x-methylstyrene oxide, fenylglycidyl ether, and glycidyl tosylate (figure 5). The enantiomerically pure product may be used for the synthesis of various other optically active compounds . Example 1 The production of styrene oxide with recombinant epoxide hydrolase Agro-bac erium radiobacter strain AD1 expressed in E. coli . The epoxide hydrolase gene was cloned by means of PCR by using degenerate primers that were designed on the amino acid sequence of the N-terminus and the C- terminus that was known from publication in literature. Total DNA of Agrobacterium radiobacter strain AD1 was isolated from cells cultivated on 1, 3-chloro-2-propanol using standard procedures. The gene of interest was amplified and cloned in an expression vector behind a T7-promotor of a plasmid such as pGEF, and brought to expressioin in E. coli . The recombinant enzyme was produced by fermentation. The whole cells were subsequently lyophilised or a crude cell -free extract was prepared by ultrasonic disruption and cetrifugation of the cells followed by a purification step with an anion exchange column. Finally the extract was dialysed against a Tris-buffer, lyophilised and stored at 4°C. Prior to use the lyophilised cell-free extract (0.50 mg) was suspended in Tris buffer (pH 9.0, 50 mM, 0.5 ml) . A flask containing a solution of 5 mM styrene oxide in Tris buffer (pH 9.0, 50 mM, 20 ml) was incubated at 30°C and the previously prepared enzyme solution was added. After 50 minutes the reaction was stopped and the remaining styrene oxide was extracted with diethyl ether. The enantiomeric purity of the styrene oxide was extracted with diethyl ether. The enantiomeric purity of the styrene oxide was determined by chiral gas chromatography on a β-cyclodextrin phase (CD- cyclodextrin-b-2,3, 6-M-19) at 95°C. The remaining (S) - enantiomer of the styrene oxide had an e.e. of 99% and a yield of 65%. Example 2 An amount of the lyophilised cell -free extract (4.5 mg) was suspended in Tris buffer (pH 9.0, 50 mM, 1 ml) . A flask containing a solution of 5 mM phenyl glycidyl ether in Tris buffer (pH 9.0, 50 mM, 100 ml) was incubated at 30°C and the prepared enzyme solution was added. After 15 minutes the reaction was stopped and the remaining epoxide was extracted with diethyl ether. The enantiomeric purity of the remaining (R) -enantiomer was determined by chiral gas chromatography using a cyclodextrin column. The remaining (R) -enantiomer of the phenyl glycidyl ether had an e . e . > 99% and a yield of 55%. Example 3 An amount of the lyophilised cell -free extract (0.7 mg) was suspended in Tris buffer (pH 9.0, 50 mM, 1 ml) . A flask containing a solution of 5 mM para-chlorostyrene oxide in Tris buffer containing 10% DMSO (pH 9.0, 50 mM, 20 ml) was incubated at 30°C and the prepared enzyme solution was added. After 15 minutes the reaction was stopped and the remaining para-chlorostyrene oxide was extracted with diethyl ether. The remaining (S) -enantiomer of the para-chlorostyrene oxide had an e . e . > 99% and a yield of 67%. Example 4 An amount of the lyophilised whole recombinant cells (5 mg) was suspended in Tris buffer (pH 9.0, 50 mM, 1 ml) . A flask containing a solution of 5 mM styrene oxide in Tris buffer (pH 9.0, 50 mM, 100 ml) was incubated at 30°C and the prepared whole cell suspension was added. After 65 minutes the reaction was stopped and the remaining styrene oxide was extracted with diethyl ether. The remaining (S) -enantiomer of the styrene oxide had an e . e . of 99% and a yield of 56%. Example 5 An amount of the lyophilised cell -free extract (100 mg) was suspended in Tris buffer (pH 9.0, 50 mM, 10 ml) . A flask containing a solution of 50 mM styrene oxide in Tris buffer with 20% (v/v) DMSO (pH 9.0 , 50 mM, 1000 ml) was incubated at 30°C and the prepared enzyme solution was added. After 125 minutes the reaction was stopped and the remaining styrene oxide was extracted with diethyl ether. The remaining (S) -styrene oxide had an e.e. > 99% and a yield of 60%. Example 6 An amount of the lyophilised cell-free extract (25 mg) was suspended in Tris buffer (pH 9.0, 50 mM, 1 ml) . A flask containing a solution of 5 mM glycidyl tosylate in Tris buffer with 10% (v/v) DMSO (pH 9.0, 50 mM, 100 ml) was incubated at 30°C and the prepared enzyme solution was added. After 20 minutes the reaction was stopped and the reamining epoxide was extracted with diethyl ether. The remaining (R) - enantiomer of the glycidyl tosylate had an e . e . > 99% and an analytical yield of 45%. Figure legends Figure 1 Complete DNA and amino acid sequence of the epichlorohydrin epoxide hydrolase gene echA from Agrobacterium radiobacter deposited at the Centraalbureau voor Schimmelcultures under number CBS 750.97. The residues shown at amino acid position 107, 246 and 275 comprise catalytic residues of epoxide hydrolase. Figure 2 Substrate for epoxide hydrolase I, wherein Xi is represented by II, III, IV or V and X 2 , Ri, R 2 , R 3 , R 4 and R 5 are independent from each other so that single as well as multiple substituents can be applied. These can be for example a hydrogen atom, a halogen atom, a nitro group, an alkyl group, a hydroxyl group, or a cyanide group. The length of the aliphatic chain can be n=0,l,2 or 3 and m=l,2 or 3. Figure 3 Sequence alignment of EchA with other hydrolases. The sequences were aligned using the multiple alignment program ClustalW and are shown in order of sequence similarity to the epoxide hydrolase cloned in this study. Six conserved amino acids are marked with #, four or five conserved residues are marked with +. The nucleophile is marked by Ν, the catalytic histidine by H, and the catalytic acid residue by A. Below the sequence alignment, the predicted secondary structure elements of EchA, and the determined secondary structures of DhlA and BpA2 are shown. β-Strands are shown as arrows (numbered as for DhlA) and α-helices are shown as waves. Sequences: EchA, epoxide hydrolase from A. radiobacter strain ADl;DehHl, fluoroacetic. acid dehalogenase from Moraxella sp. strain B;sEHs, soluble epoxide hydrolase from potato; sEHh, soluble epoxide hydrolase from human; BpA2 , bromoperoxidase A2 from Streptomyces aereofaciens; DhlA, haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 and mEHh, microsomal epoxide hydrolase from human. Figure 4 Topology of the α/β hydrolase fold of epoxide hydrolase from Agrobacterium radiobacter . Arrows indicate β-strands, boxes indicate α-helices. The numbers printed in large font indicate β-strands, those in small font indicate the residue numbers according to the amino acid sequence. The positions of the three catalytic triad residues is indicated. Figure 5 Kinetic resolution of aromatic epoxides by epoxide hydrolase from Agrobacterium radiobacter a) determined by injection of optically pure compound b) determined by optical rotation c) in 10% DMSO. References Arand M., Grant D.F., Beetham J.K., Friedberg T., Oesch F. , Hammock B.D. (1994). FEBS Lett . 338, 251-256. Archer, I.V.J. (1997), Tetrahedron, 53, 15617-15662 Beetham J. K., Grant D., Arand M. , Garbarino J. , Kiyosue T., Pinot F., Oesch F. , Belknap W. R.,Shinozaki K., Bont, J. A. M. de. (1993) Tetr. Asymm . 6, 1331-1340. Watabe, T., Ozawa, N., Hiratsuka, A. (1983) Biochem . Pharm . 32, 777-785 Chen, X. J. , Archelas, A., Furstoss, R. (1993) J. Org . Chem . 58, 5528-5532, and 5533-5536. Nelliah, H., Morisseau, C, Archelas, A, Furstoss, R, Baratti,J. C. (1996) Biotech . Bioeng. 49, 70-77. Nakamura, T, Nagasawa, T, Yu, F., Watanabe, I., Yamada, H. (1994) Appl . Environ . Microbiol . 12, 4630-4633 Pedragosa-Moreau, S.,. Morisseau, C, Zylber, J. , Archelas, A., Baratti, J., Furstoss, R. (1996) J. Org. chem . 61, 7402-7407 Guengerich, F. P. (1982) Rev. Biochem . Toxicol . 4, 5-30. Beetham, K. B., Grant, D., Arand, M. , Garbarino, J. , Kiyosue, T., Pinot, F. , Oesch, F. , Belknap, W.R., Shinozaki, K., Hammock B.D. (1995) DNA Cell Biol 14, 61-71. Hechtberger, P., Wirnsberger, G., Mischitz, M. , Klempier, N., Faber, K. (1993). Tetr . Asymm . 4, 1161-1164. Jacobs, M. H. J. , A. J. van den Wijngaard, M. Pentenga, D. B. Janssen. (1991). Eur. J. Biochem . 202, 1217-1222. Lacourciere, G. M. , Armstrong, R. N. (1993) J. Am . Chem . Soc. 115, 10466-10467. Hand, R. H. , Jakoby, W .B. (1969) J. Biol . Chem . 244, 2078- 2084. Pinot, F., Grant, D. F., Beetham, J. K. , Parker, A. G., Borhan, B., Landt, S., Jones, A. D. , Hammock B. D. 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Claims: CLAIMS 1. An isolated micro-organism capable of selectively degrading epichlorohydrin or related halopropanol compounds, said micro-organism being a representative of Agroba cterium spp and comprising a nucleic acid molecule encoding a polypeptide having enantioselective epoxide hydrolase activity. 2. A micro-organism according to claim 1, said microorganism essentially corresponding to the micro-organism Agrobacterium radiobacter deposited May 7, 1997 at Centraalbureau voor Schimmelcultures under deposit number CBS 750.97. 3. A pure culture, or a preparation thereof, of a microorganism according to claim 1 or 2. 4. A crude or pure enzyme preparation, comprising a polypeptide or functional fragments thereof, having epoxide hydrolase activity, said polypeptide derived from a microorganism according to claim 1 or 2. 5. A polypeptide or functional fragments thereof, having epoxide hydrolase activity, said polypeptide (fragment) derived from a micro-organism according to claim 1 or 2. 6. A polypeptide having enantioselective epoxide hydrolase activity, said polypeptide having a catalytic mechanism based on a catalytic triad formed by two aspartate and one histidine groups, preferably as defined in figure 1. 7. A polypeptide according to claim 5 or 6, having an amino acid sequence that corresponds for at least 90% to the sequence of figure 1. 8. A recombinant DNA molecule comprising a nucleotide sequence encoding at least a functional part of a polypeptide having epoxide hydrolase activity. 9. A recombinant DNA molecule according to claim 8, wherein said polypeptide exhibits enantioselective activity. 10. A recombinant DNA molecule according to claim 8 or 9, said nucleotide sequence being derived from a micro- organism according to claim 1 or 2. 11. A recombinant DNA molecule comprising at least a functional part of the sequence of figure 1, preferably at least 90%, and coding for a polypeptide having epoxide hydrolase activity. 12. A recombinant DNA molecule according to claim 11 wherein said polypeptide exhibits enantioselective activity. 13. A recombinant DNA molecule according to any of claims 9 to 12 wherein at least one codon is mutated to encode for an amino acid providing the gene product with a modified epoxide hydrolase activity. 14. A vector for expression of a polypeptide (fragment) having epoxide hydrolase activity in a host cell comprising a recombinant DNA molecule according to any one of claims 7-13. 15. A host cell for expression of a polypeptide (fragment) having epoxide hydrolase activity comprising a vector according to claim 14. 16. A host cell according to claim 15 which is a bacterium or a yeast cell. 17. A method for producing a polypeptide (fragment) having epoxide hydrolase activity comprising the use of a micro organism according to claim 1 ore 23, or a host cell according to claim 15 or 16. 18. A pure culture, or a preparation thereof, of a host cell according to any claims 15 or 16. 19. A crude or pure enzyme preparation, comprising a polypeptide or functional fragments thereof, having epoxide hydrolase activity, said polypeptide derived from a host cell according to claim 15 or 16. 20. A polypeptide (fragment) having epoxide hydrolase activity obtainable by a method according to claim 17. 21. A polypeptide (fragment) according to claim 20, wherein said polypeptide exhibits enantioselective activity. 22. Use of a polypeptide (fragment) according to claim 5, 20, or 21 to stereoselectively convert racemic mixtures of optically active epoxides to diols. 23. Use of a micro-organism according to claim 1 or 2, or a host cell according to claim 15 or 16, or a culture, or preparation thereof, according to claims 3 or 16 or a enzyme preparation according to claims 4 or 19 to fully of partly and stereoselectively convert racemic mixtures of optically active epoxides to diols. 24. Use according to claims 22 or 23 to prepare enantiopure epoxides and diols. 25. Process for the enzymatic hydrolysis of epoxides, said process comprising contacting a racemic epoxide with a polypeptide according to claim 5, 13 or 21 and/or a microorganism according to claim 1 or 2, or a host cell according to claim 15 or 16, or a culture, or preparation thereof, according to claims 3 or 18 or a enzyme preparation according to claims 4 or 19 to fully of partly and stereoselectively convert racemic mixtures of optically active epoxides to diols. 26. Process for the preparation of substantially pure optically active epoxides and/or diols by stereospecifically hydrolysing a racemic mixture of epoxides using a broadly specific microbial epoxide hydrolase yielding preparations of optically active epoxides or diols with at least 95% e.e. 27. Process for the preparation of substantially pure optically active epoxides and/or diols by stereospecifically hydrolysing a racemic mixture of epoxides using a microbial epoxide hydrolase yielding preparations of optically active epoxides with at least 99% e.e. 28. Process for preparing enantiomerically enriched epoxides and/or diols, comprising contacting a racemic epoxide of the formula wherein Rl and R2 are independently selected from the group of hydrogen and organic radicals with an isolated microorganism capable of selectively degrading epichlorohydrin or related halopropanol compounds, said micro-organism being a representative of Agrobacterium spp and comprising a nucleic acid molecule encoding a polypeptide having enantioselective epoxide hydrolase activity. 29. Process according to claim 28, wherein Rl and R2 are selected from the group of hydrogen and, optionally substituted, straight or branched alkyl, alkenyl, aryl, aralkyl, alkaryl groups.

Inventors: JANSSEN DICK BAREND, KELLOGG RICHARD MORRISON, LUTJE SPELBERG JEFFREY HARALD, RINK RICK, JANSSEN, DICK, BAREND, KELLOGG, RICHARD, MORRISON, LUTJE SPELBERG, JEFFREY HARALD, RINK, RICK
Applicants: JANSSEN DICK BAREND, KELLOGG RICHARD MORRISON, LUTJE SPELBERG JEFFREY HARALD, RINK RICK, UNIV GRONINGEN, JANSSEN, DICK, BAREND, KELLOGG, RICHARD, MORRISON, LUTJE SPELBERG, JEFFREY HARALD, RIJKSUNIVERSITEIT TE GRONINGEN, RINK, RICK
Application year: 1998
Main classification: C12N15
IPC codes: C12N9, C12N15, C12P7, C12P41