LUMINESCENCE ASSAYS

Abstract: Disclosed are apparatus and method of detection of luminescence in which a luminescent species of interest is excited by a process of simultaneous or sequential absorption of at least two photons from at least one photon source, wherein the or each photon source is modulated at one or more frequencies, and luminescence measurements are made at a frequency or frequencies different from the or each modulation frequency, the or each frequency at which measurements are made being a function of the or each modulation frequency by virtue of the excitation process.

Description: LUMINESCENCE ASSAYS The present invention relates to luminescence assays based on transfer of excitation energy from a donor species to an acceptor species. Fluorescence, phosphorescence and related technologies (herein referred to as "luminescence" to include in this context all processes where energy is emitted subsequent to an excitation process triggered by absorption of electromagnetic radiation) are now widely used in a variety of analytical schemes. Luminescent materials are used as tracers on the basis of the high detection sensitivity that can be achieved, but are also used as environmentally responsive "probes" to monitor local conditions, such as pH, ion concentrations, oxygen tension etc. Luminescent species can also be used to detect and sometimes quantify the proximity of an agent which is able to modify the emission process on close approach or contact. It is well known that energy can be transferred by a variety of means from an excited species (the "donor") to a second species able to act as an energy acceptor. One of the most common examples of such transfer involves a radiationless process known as resonance energy transfer, the efficiency of which usually has an inverse sixth power dependence on distance between donor and acceptor. Distance-dependent energy transfer between donor and acceptor species has been used in a variety of analytical and assay formats. An analyte might be detected on the basis of its ability to bind to a site where it can function as one member of the energy transfer donor- acceptor pair. Alternatively, the assay might be conducted in a competitive format where the analyte displaces a labelled analogue from a site and the displacement can be detected and quantified by changes in energy transfer between the site and the analogue. One common type of assay involves the detection of an analyte on the basis of its ability to bind two recognition molecules such as antibodies simultaneously. In this "sandwich" format, the proximity of the two bound species can be determined by energy transfer between labels bound to the antibodies. A recent example of an energy transfer assay using a luminescent cryptate of long emissive lifetime to transfer energy to a short-lived acceptor species is given by G. Mathis ("Rare Earth Cryptates and Homogeneous Fluoroimmunoassays with Human Sera", Clinical Chemistry, Volume 39(9), 1953-1959, (1993)). Another approach to the assay of enzymes or similar catalytic species (e.g. ribozymes) relies on the ability of the analyte to cleave a chemical bond linking an energy transfer donor to an acceptor species. For example, a protease can be assayed by monitoring the decrease in energy transfer efficiency between donor and acceptor linked together by a peptide fragment. As the linkage is broken the donor and acceptor become separated and efficient transfer of energy is no longer possible. Conversely, an analyte able to initiate chemical bond formation might be assayed on the basis of increase in energy transfer between a suitable donor and acceptor pair as they become linked together. These and similar methods of assay based on transfer of excitation energy are well known in the literature. For a general discussion of analytical applications of luminescence in Biology see for example "Applications of Luminescence Spectroscopy to Quantitative Analyses in Clinical and Biological Samples", P. Froehlich in "Modern Fluorescence Spectroscopy" Volume 2, E.L. Wehry ed., Plenum, N.Y. (1976). Details of a variety of fluorescence assay procedures are given in "Principles and Practice of Fluoroimmunoassay Procedures", D.S. Smith, M. Hassan and R.D. Nargessi in Volume 3 of the same series. Most assays involving energy transfer processes are relatively insensitive and can have limited dynamic range. One problem with all luminescence detection is the sensitivity limit set by background. In this context, background might be unwanted luminescence from sample or container, elastically or inelastically scattered exciting light which is not totally rejected by optical filters, luminescence from filters themselves or from other optical elements or any other source of electromagnetic radiation detectable by the measuring apparatus. In absence of background, luminescence methods are commonly able to detect single atoms or molecules. Most analytical luminescence measurements fall short of this sensitivity by orders of magnitude and therefore methods to minimise background are very important in practical applications. Unfortunately, luminescence energy transfer is commonly troubled by background from a variety of causes. In a practical measurement of energy transfer, ideally the donor is excited by radiation not absorbed by the acceptor. The transfer is detected either by measurement of quenching of donor emission (or related parameters such as change in donor decay rate), or by the sensitised emission from the acceptor if this is also luminescent. Ideal conditions are rarely achieved however and the following potential difficulties and sources of background must be considered: • It is often difficult to avoid some level of direct excitation of the acceptor species by radiation used to excite the donor. In assays where the acceptor is luminescent, this means that there is usually a background of luminescence from the acceptor, even in the absence of any energy transfer from the donor and this in turn limits dynamic range of any assay using the donor-acceptor pair. • Exciting radiation is capable of exciting luminescence from impurities or other unwanted components of the sample. The donor might emit some radiation at a wavelength which overlaps with the passband of the optical filters used to isolate emission from the acceptor, so that a signal is detected in absence of energy transfer. These and related problems limit the scope, sensitivity and dynamic range of assays based on energy transfer processes. It is therefore an object of the present invention to obviate or mitigate the abovementioned disadvantages. According to the present invention there is provided a method of performing an energy transfer luminescence assay to determine an analyte in which a donor species is irradiated to raise the donor species to an excited state, and the analyte causes a change in the excitation condition of at least one of the donor species and an acceptor species and said change is monitored to determine the analyte wherein (i) the donor species is provided as, in or adsorbed to a solid phase and is an up- conversion medium capable of effecting, by energy pooling or otherwise, an energy transition to an excited state by absorption of electromagnetic radiation having an energy less than that of said transition, (ii) the acceptor species is bound or is capable of being bound directly or indirectly to the surface of said solid phase, (iii) the donor species is irradiated with said electromagnetic radiation, and (iv) luminescence is detected in at least one spectral region characteristic of the emission of the donor species and/or the acceptor species provided that the excitation of the acceptor species to an excited state capable of luminescence in a defined spectral region of the acceptor species does not occur by absorption of a single quantum of the radiation used to excite the donor species. The invention is based, therefore, on the use as the donor species of an up- conversion medium which is capable of undergoing (by energy pooling or otherwise) an energy transition to the excitation state required for use in the assay as a result of excitation by photons which are of lesser energy than said transition. A further feature of the invention is that if an excitation condition of the acceptor species is monitored then such monitoring is in a spectral region which is a characteristic of the acceptor species and in which the latter has not been excited to a luminescent state by absorption of a single quantum of the radiation used to excite the donor. This ensures that, in the defined spectral region of the acceptor, any change in the excitation condition is due to energy transfer from the donor species and not due to direct excitation of the acceptor species by the electromagnetic radiation. This is easy to achieve given the relatively low energy of the electromagnetic radiation which may be used to excite the donor species. These features provide the advantage that the relatively low energy of the excitation photons minimises, and possibly eliminates, background problems and does not give rise to excitation of the acceptor in the absence of energy transfer from the donor species to acceptor species bound thereto. A solid species has several advantages as an energy transfer donor. The solid matrix prevents or minimises collisional quenching of the donor emission by dissolved species such as ions or oxygen, and limits access of the luminescent species to water. Water can be an efficient quencher of many long-lived luminescent species, at least in part on account of its relatively high phonon energy which facilitates radiationless deactivation. The solid matrix provides a relatively rigid environment conducive to long emission lifetime and high luminescence efficiency. Long lived excited states are appropriate to the present invention in that they are efficient as energy transfer donors by virtue of diffusional enhancement of transfer to species notionally bound at distances where otherwise energy transfer would be negligible, and are well suited to lifetime resolved detection methods which minimise background. In addition, the long lifetime of metastable intermediate states is important in multiphoton excitation schemes. The solid matrix also provides a potential means to enhance sensitivity by allowing energy migration between luminescent species in the matrix. This allows distant excited species to contribute to energy transfer to a surface bound acceptor by first transferring energy to intermediate species until the energy reaches a site sufficiently close to the acceptor for efficient transfer to occur. A further advantage of a solid medium is evident in that it provides a surface to which a wide variety of other species can be bound by standard protocols. This facilitates attachment of recognition ligands and similar molecules related to the analytical application. For example, antibodies, lectins, oligonucleotides, nucleic acids, biotin, proteins such as avidin or streptavidin or other materials able to bind selectively to a complementary substance might be required in a given analytical protocol. The up-conversion medium may be one which undergoes the transition to its exited state by simultaneous or sequential absorption of two or more photons of the same or different energy, each of said photons being of lower energy than the transition and not being able to excite the acceptor species. The up-conversion medium may be one requiring a priming dose of energy to excite species therein to metastable levels which are required for the up-conversion process to be possible. For example, the up-conversion medium may be an electron trapping phosphor. The up-conversion medium may be one relying on excitation of an organic molecule to an excited state which subsequently relaxes to a metastable level (e.g. a triplet state of the organic molecule) of longer lifetime than the original state. Subsequent absorption of a second photon may promote re-excitation of the metastable state to the excited state of the donor species which is capable of luminescence or transfer of energy to the acceptor. Alternatively, intersystem crossing from the metastable level may give rise to an excited state of different spin multiplicity that is itself capable of luminescence or transfer of energy to an acceptor. The up-conversion process may be based on the excitation of lanthanide ions in an appropriate matrix. The up-conversion medium may be such that exciting radiation is absorbed by one or more species and subsequently transferred to one or more other species which acts as the donor. In this case, the primary absorbing species may, for example, be the ytterbium ion and the donor species may be erbium or thulium ions. The donor species which is excited by the up-conversion process may be one which is able to delocalise its excitation by internal transfer of energy between similar sites within the up-conversion medium. This delocalisation of excitation may result in transfer of energy from sites within the bulk of the medium to sites at or near the surface of the medium which are subsequently able to transfer energy to the acceptor species. For use in the assay of the invention the solid phase which is or which incorporates the donor species is preferably in the form of a particle, most preferably one having a size of 10-100 nm. The acceptor species may be one which is capable of luminescence on energy transfer from the donor. As conventional in energy transfer assays, the transfer can only occur when the donor and acceptor species are in sufficiently close proximity as provided for by the binding of the acceptor to the donor. In a first preferred embodiment of the invention, the assay may be one in which the analyte causes a change in the extent of binding between the solid phase of the donor species and the acceptor species. The assay may be conducted in a format in which the analyte causes an increase in the degree of binding of the acceptor to the donor. In this case, the change which is monitored in either the acceptor species or donor species is as a result of energy transfer from the donor to the acceptor. One example of such an assay format is one involving analyte mediated binding of the acceptor to the donor. Thus, for example, the surface of the phosphor may be provided with analyte recognition molecules (e.g. antibodies, oligonucleotides or lectins) and the acceptor may be linked to a species (e.g. a further antibody) capable of binding to the combination of the analyte and the analyte recognition molecule on the donor. Alternatively the action of an enzyme or other catalytic species may be monitored by activation of coupling between donor and acceptor, either by catalysing formation of a linkage between them or by unmasking of protected groups on either or both of the donor and acceptor species, resulting in formation of a complex between the said species or formation of a chemical bond between them. Alternatively the assay may be conducted in a format in which the analyte causes a decrease in binding of the acceptor to the donor. In this case, the change which is monitored in either the acceptor species or donor species is representative of a decrease in energy transfer from the donor. Thus, for example, for use in the assay the acceptor may be displaceably bound to the donor and the analyte is capable of displacing the acceptor thus providing a decrease in binding. An example of such an assay would be the use of an enzyme to catalyse the cleavage of a linkage between donor and acceptor, resulting in a decrease of binding between them. In a second preferred embodiment of the invention, the analyte may be one which affects the acceptance properties (for energy transfer from the donor species) of a moiety which is bound to the solid phase of the donor species, and/or which affects the ability of the bound moiety to emit luminescence consequent on excitation by said energy transfer. For example, in accordance with this second embodiment: (a) The assay may be used to monitor the formation or cleavage of a bond linking a quenching species or an enhancer of emission to the solid phase; or to a luminescent moiety bound thereto: (b) The moiety may be one which is converted by the analyte to a luminescent species capable of accepting energy from the excited donor species. The moiety may, for example, be a pro-fluorophore: (c) The moiety may be one which is a pre-existing luminescent label (excited by energy transfer from the donor species) whereof luminescence is quenched by the action of the analyte: (d) The moiety may be one which is able to change colour as a consequence of the action of the analyte. In this case, the excited up-conversion medium is able to luminescence with an efficiency determined by the colour of the bound agent. In all cases, energy transfer from the donor species to the acceptor may be determined by measurement of quenching of donor emission (or a related parameter such as change in donor decay rate) or by sensitised emission from the acceptor if this is also luminescent. The donor species is preferably an infra-red up-conversion phosphor. Such phosphors are commonly inorganic crystals or glasses designed to absorb infra-red radiation and to give rise to visible luminescence and are commonly used for detection of emission from infra-red laser sources. In the present invention however, some or all of the excitation energy from the phosphor is transferred radiationlessly to the acceptor species and such transfer can be detected either as a quenching of the visible luminescence of the phosphor, or by luminescence from the acceptor species if the said species is capable of such emission. A variety of up-conversion phosphors are known and fall broadly into two categories. One type is based on use of an electron-trapping phosphor where the phosphor is "charged" by exposure to visible or ultraviolet light or other suitable radiation and where infrared light empties the traps giving rise to emission as a result of recombination processes. Such phosphors are usually chemically unstable in aqueous media and typically require encapsulation in a polymer. In addition, they exhibit a time-dependent background glow after charging and thus are not ideal for present purposes. This type of phosphor will not be considered further, though it could in principle be used in an appropriately designed assay, especially if pulsed excitation and gated detection were combined to minimise the effects of the background glow. This is possible because the background emission of the electron- trapping phosphor typically has a very long decay time extending to many hours in some cases, while the luminescence emitted in response to long wavelength excitation is much shorter lived and can be easily discriminated from background on this basis. A more suitable candidate is the energy-pooling phosphor where an emitting species is excited by sequential absorption of quanta of energy, either directly or more usually via a sensitising agent. Such phosphors have recently been proposed for solid- state three-dimensional displays activated by multiphoton absorbence (see "A Three- Color, Solid-State, Three-Dimensional Display" by E. Downing, L. Hesselink, J. Ralston and R. Macfarlane, Science, Volume 273, 30th August 1996, pages 1 185- 1 189). Efficient systems are typically based on heavy metal fluoride-containing glasses of a variety of compositions designed to give low lattice phonon energy and hence allow efficient emission from ions in the matrix. The cited article discusses efficient phosphors giving rise to red emission (based on trivalent praesodymium ion as dopant), blue emission (based on trivalent thulium ion as dopant) and green emission (based on trivalent erbium ion as dopant), and discusses energy level diagrams for the upconversion processes in these materials. As sensitising agent, trivalent ytterbium ions are often used. A ytterbium-sensitised, erbium-doped system giving rise to green light on excitation at 900-lOOOnm via a two-photon process and a ytterbium-sensitised, thulium doped system giving blue emission via a three-photon process have been known for many years. Other ions such as neodymium have also been used as sensitisers, particularly in the context of fibre-optic upconversion media for laser applications. In phosphors such as these, a sensitising agent transfers energy to a metastable state of the emitter which can, after suitable relaxation, accept further quanta leading to a higher excited state which emits at shorter wavelength than the exciting light. The phosphors themselves are typically stable glasses which can be fabricated as sub-microscopic particles by standard methods, or can be surfaces where the emitting species is implanted by diffusion or bombardment. For use in some types of assay it is convenient to be able to derivitise or otherwise modify the surface of the up-conversion medium to permit recognition agents such as antibodies and lectins to be attached. There are many standard methods to achieve this, ranging from simple physical adsorption of polymers, proteins etc. onto the surface to covalent attachment via linking groups such as organosilanes which bind to surfaces bearing hydroxyl groups. The glassy matrices typical of inorganic up-conversion phosphors are quite suitable for such modification. Some inorganic glasses which are otherwise suitable as efficient upconversion media might not be stable in water or might suffer quenching at the surface when present as very small particles for assay purposes. In such cases, a protective molecular layer of a chemically - or physically adsorbed material would be advantageous to prevent or minimise access to water. Organosilanes with hydrophobic chains bearing terminal reactive groups for example can combine the role of surface protection from water and activation for ligand coupling. A further possible implementation of the invention uses organic or inorganic polymers as the host for the up-conversion process. In this case it is important to ensure that the polymer is not able to deactivate the excited species too rapidly for the up-conversion process to operate. Typically, polymers with low phonon energy states are most suitable in this context. It is also possible to use organic dyes in an up-conversion process. Two- photon processes and three-photon processes are well known in this context, but usually require a very high power density for useful efficiency on account of the low cross section for simultaneous multiphoton absorption. Examples where photons are absorbed sequentially are also well known and these can be efficient at much lower power densities than are required for coherent multiphoton processes. Anti-Stokes fluorescence as a result of either triplet recombination or triplet re-excitation to excited singlet has been known for many years and these processes are well understood. Triplet re-excitation to the excited singlet through intersystem crossing from vibrationally-excited levels gives rise to temperature-dependent delayed fluorescence and has been used in optical thermometry (J.C. Fister et al, Anal. Chem. 1995 67 4269-4275). This publication points out that the rate of intersystem crossing is much slower than the rate of thermal equilibration between vibrationally-excited levels, so that the vibrational levels are populated according to the Boltzmann distribution. Any attempt to populate the excited singlet by excitation of vibrational levels of the triplet through photon absorption is likely to be inefficient on this basis, because the excess vibrational excitation will be lost within picoseconds. However, in some circumstances the excited singlet can be populated through intersystem crossing from a higher energy triplet state excited by means of the allowed triplet-triplet transition and this can be an efficient process in principle. Organic dyes can be used both in inorganic matrices such as sol-gel glasses and in organic polymer matrices. Immobilisation of dyes in such matrices usually increases the lifetime of triplet states markedly and this increases the probability of further absorption to excite the triplet back to the excited single manifold. Although immobilisation might be expected to decrease the probability of up-conversion processes based on triplet-triplet annihilation, this need not be so because dyes can form clusters in the matrices and annihilation processes can be highly efficient in these circumstances. In some cases the polymeric matrix or a co-dissolved dopant can act as an energy donor to the luminescent organic material and excitation energy can be delocalised, for example by exciton migration within the matrix. Such systems allow energy to be transferred to sites which are remote from the point of primary absorption. In the method of the invention, detection of the excitation condition of the donor species and/or acceptor species may, for example, be effected by a semiconductor diode, a linear charge-coupled device (CCD), MOS sensor or an imaging detector. Such an imaging detector may utilise an image intensifier or imaging single photon detector. Alternatively the imaging detector may be a CCD camera or other solid state imaging device. It is well known that multiphoton excitation can be achieved using a continuous light source of sufficient intensity ^Two-photon excitation fluorescence microscopy using a semiconductor laser", P.E. Hanninen et al, Bioimaging. (1995). 3(2), 70-75), and that such excitation can be achieved without unacceptable damage to a specimen. According to the above publication, a principal limitation on the optical power which could be applied to a sample was set not by photochemical damage, but by the "optical tweezer" effect where intense beams of radiation exert forces on microscopic objects. The main difficulty in the use of a continuous excitation source is the requirement to reject background signals, since radiation is present at all times during the measurement. This background rejection need not be a problem if the detector used does not respond at the exciting wavelength(s). Where this is impractical the exciting radiation often can be rejected very efficiently by optical filters. For example, as described in "Time-Resolved Fluorescence Microscopy using Two- Photon Excitation , P.T.C. So et al, Bioimaging, (1995), 3(2), 49-63 a simple filter based on a solution of copper sulphate achieved an effective optical density of 20 for an exciting long wavelength radiation (i.e. the ratio of incident to transmitted light 20 was 10 ) while transmitting approximately 70% of a two-photon-excited fluorescence which was to be detected. Nevertheless, such efficient optical rejection cannot always be realised, and other methods to reject scattered light are sometimes required. One such means to achieve selective detection of upconverted luminescence is to exploit the non-linearity of the excitation process. This leads to a further aspect of the present invention according to which there is provided a method of detection of luminescence in which a luminescent species of interest is excited by a process of simultaneous or sequential absorption of at least two photons from at least one photon source, wherein the or each photon source is modulated at one or more frequencies, and luminescence measurements are made at a frequency or frequencies different from the or each modulation frequency, the or each frequency at which measurements are made being a function of the or each modulation frequency by virtue of the excitation process. A single excitation source may be provided, the source being modulated in for example intensity at a frequency F. Any background of scattered light originating from the source will be modulated at the same frequency. Any luminescence excited by a single photon process will also be modulated at F, with a relative modulation depth determined by the frequency of modulation in relation to the lifetime of the excited state of the single photon process. Although fluorescence excited by a long wavelength radiation is normally of lower energy than the excitation, shorter wavelength "Anti-Stokes" emission is possible and is sometimes seen where high intensity excitation is used. If however a two-photon excitation process is occurring, then resulting emissions will have a component fluctuating at 2F (higher frequencies will occur for higher order multiphoton processes). Relative modulation depths of each component will depend on the lifetime of the excited states relative to the modulation frequency, and a suitable frequency may be chosen to give appreciable modulation of a desired excitation process. It is a simple matter to detect a signal fluctuating at 2F in the presence of a constant background or signal fluctuating at F. In principle the above single frequency technique is a powerful means to reject background frequencies through multiphoton excitation. However, a potential difficulty may be the presence of harmonic distortion in the modulated light source. If such distortion is present there may well be a component of excitation fluctuating at 2F, 3F or other multiples of the fundamental. Scattered light or ("Anti-Stokes") single-photon-excited fluorescence will then be detected at harmonics of the fundamental as if it were the desired multiphoton-excited emission. Most techniques used to modulate light give rise to some level of harmonic distortion, and the introduction of harmonic distortion will always be a potential problem. In order to overcome such potential problems resulting from harmonic distortion, it is preferred to use a single source modulated with a composite waveform comprising the sum of two or more different frequencies, measurements being made at a frequency which is a linear combination of the different frequencies. Digital or analogue techniques may be used to achieve the desired modulation and in the former case the luminescence measurement may be recovered conveniently by Boolean logical analysis of a detected signal. The method of detection is particularly suited to an emitting species with a long luminescence decay time such that emissions excited by the source or sources are not able to reproduce the modulation frequencies but are able to reproduce frequencies corresponding to a difference frequency, measurement being made at the difference frequency. This allows the presence of an interfering species capable of luminescence as the result of multiphoton excitation processes, but with a shorter luminescence lifetime than the species of interest, to be detected. Measurements are made at one or more frequencies selected such that a signal from the species with the long decay time will suffer substantial attenuation by virtue of demodulation while a signal from the interfering species will not suffer substantial attenuation. Suitably, measurements are made at frequencies that are the sum and difference of the modulation frequencies, and a function of the result of the measurement at the sum frequency is subtracted from a function of the result of the measurement at the difference frequency to suppress contributions from short-lived emissions. In some cases it may be necessary or experimentally convenient to excite upconverted luminescence by illuminating the sample with two or more light sources of the same or different wavelength. For example, two excitation sources may be chosen so as to match the energies of individual electronic transitions involved in the upconversion process, or light sources of the same wavelength may be focused onto a common point. In such cases the individual sources can be modulated and measurements of luminescence made at one or more frequencies present by virtue of the non-linearity and/or multiplicative nature of the upconversion process. The exciting radiation may be provided by a semiconductor light-emitting diode or laser. The exciting radiation may be pulsed or modulated in intensity in which case gated detection may be used to confine detecting signals to one or more defined time period relative to the excitation. The detection system may use a periodically switched, gated or modulated detector to implement lock-in detection or similar correlated detection schemes. For example, the detector may be an imaging detector which is gated or modulated in sensitivity. Alternatively an imaging detector may be designed to implement phase sensitive detection in conjunction with a periodic excitation source. An up-conversion phosphor as described above is particularly preferred for use in the invention since it solves many of the background problems when used as a component in an energy transfer system. * The exciting radiation is not sufficiently energetic to excite background from the sample or surroundings at a wavelength which will interfere with the measurement. * Suitable sources of high intensity pulsed near infrared radiation are very low cost solid state devices. * Detectors such as photomultipliers are insensitive to the near infrared radiation, which can in any case be rejected very efficiently by low cost optical filters. * The visible emission from the phosphors is highly structured, facilitating spectral discrimination in the event of any overlap between donor emission and that of the acceptor. * The excited state lifetime of the phosphor is relatively long. Consequently the method lends itself naturally to lifetime-resolved detection. This mode of operation is insensitive to stray light, so that background from this source is greatly reduced. * When time gating is used with a pulsed light source a simple photodiode will give good detection efficiency, because the signal is concentrated in a short pulse and dark current can effectively be ignored. Although the diode might have some sensitivity to any residual infrared light leaking through optical filters, time gating will eliminate this. * In addition to the detection of luminescence using a photodiode or photomultiplier, the method lends itself to detection using an imaging detector or linear array. Suitable detectors include image intensifiers (which can additionally be gated or modulated in sensitivity), linear MOS sensors or CCDs and various types of semiconductor area detector such as CCD cameras. Some types of CCD camera can be gated, for example by control of the active imaging period by suitable manipulation of the charge drain facility in conjunction with the charge storage and transfer facilities of the camera. Alternatively, the camera can be equipped with an optical shutter capable of single or periodic operation. Suitable liquid crystal shutters are presently available and have been used previously for phosphorescence detection with a CCD camera. Imaging detection of luminescence in the context of the present application is appropriate for example to simultaneous measurement of samples in multiwell plates, such as are widely used in immunoassays and similar analytical applications. In such applications it might be advantageous to illuminate the sample with an array of semiconductor light-emitting diodes to furnish the required long wavelength excitation, though of course other sources of photons such as pulsed flashlamps could also be used. * The excitation process is proportional to the square or higher power of the infrared exciting radiation. This means that in a focused beam, emission is mainly seen from the focal plane. This is potentially advantageous in an assay, because it avoids problems of signal originating from the container or from background outside the focal region. It is also possible to excite the sample simultaneously with two or more sources of photons of differing wavelength, in which case the efficiency of excitation will be proportional to the product of the individual photon power densities (or to the product of the square, cube or other exponents of each of the photon densities). Some types of up-conversion media have a further advantage when used as the donor species and this is particularly (though not exclusively) so with those phosphors in which the up-conversion process involves lanthanide ions within a suitable matrix. Within the up-conversion medium, energy is transferred by a near-resonant process between ions of the emitting species. This very efficient transfer means that the excitation is effectively delocalised. If an energy acceptor approaches closely enough to any ion for efficient energy transfer to be possible, it is also possible for energy from a more distant ion to migrate through the lattice and reach the acceptor which acts as an energy "sink". This means that a single acceptor can be excited by ions which otherwise would be too far away for energy transfer to be efficient. The process gives rise to an amplification mechanism, since a given acceptor can be excited many times from a given excitation pulse. In practical terms the effect is also advantageous, because otherwise only very small phosphor particles would be efficiently quenched by energy transfer to the acceptor. Similarly, in the case of an assay format involving detection of binding to an extended surface, such as for example the surface of an optical fibre doped with up-conversion medium, the effect allows energy to be accepted from a greater depth within the surface than would otherwise be the case. Although the most commonly encountered mechanism of energy transfer between organic species is a dipole-dipole mechanism, other mechanisms are possible including multipole effects, electron exchange processes and surface effects involving plasmons. For this reason, suitable energy transfer acceptors are not limited to simple organic dyes or fluorophores but may be inorganic substances (e.g. colloidal inorganic semiconductors such as zinc selenide) which have an absorption spectrum overlapping the emission from the donor species or which can act as energy acceptors by other means such as electron exchange. Colloidal metals (e.g. colloidal gold and silver) are also potential acceptors for energy from the donor. Colloidal gold is commercially available derivatised with a variety of surface-bound ligands such as antibodies for applications in conventional assay procedures. Amplification processes such as the widely-used silver enhancement method for colloidal gold labelling might also be applied to increase the effectiveness of such labels as acceptor species by increasing the amount of bound colloid and inducing greater absorbence at the particle surface. The invention will be further described by way of example only with reference to the accompany drawings, in which: Fig. 1 schematically illustrates (not to scale) an up-conversion phosphor involved in analyte mediated binding of a fluorescent dye as acceptor species in accordance with the first preferred embodiment of the invention Fig. 2 illustrates the emission from a Ytterbium-sensitised Erbium-doped upconversion phosphor. Fig. 3 illustrates the situation where the donor species has independent emission transitions which are differently affected by energy transfer to an acceptor species; Fig. 4 schematically illustrates assays in accordance with the second preferred embodiment of the invention; and Fig. 5 is a schematic diagram representing a configuration of apparatus for detecting upconverted luminescence. Referring to Fig. 1 , there is illustrated a phosphor particle 1 having a size of 10 to 100 nm comprised of a mixture of Ytterbium and Erbium ions in a glassy matrix. Attached to the outer surface of particle 1 by covalent bonding or adsorption are antibodies 2 to which an analyte molecule 3 will bind. As further illustrated in Fig. 1, an acceptor species 4 (in the form of a dye molecule) is bonded to further antibodies 5 which are capable of binding to the complex formed between the analyte 3 and the antibodies 1 such that the acceptor species (now located within close proximity of the particle 1) is capable of having energy transferred thereto from an excited state of the phosphor. The emission from a typical Ytterbium-sensitised Erbium-doped upconversion phosphor excited at 980nm is shown in Fig. 2 below. As is evident, there is a clear spectral region where luminescence from an energy acceptor could be measured without interference from phosphor emission. In the case of this phosphor dyes such as Rhodamine 6G, sulforhodamine 101 and Cresyl Violet would be suitable as energy acceptors with fluorescence in the region where the phosphor does not emit, though of course there are many other fluorophores which could be used. In the example shown, there are two well separated emission peaks. In case where such transitions are independent it is likely that the emission peaks will be quenched to different extents by energy transfer to an acceptor, particularly where the absorption of the acceptor overlaps one emission peak more than the other. This is shown in Figure 3 which illustrates the situation where the donor species has independent emission transitions which are differently effected by energy transfer to an acceptor species, e.g. a case where the acceptor absorbence is greater for one emission peak than for the other. In Figure 3a, the peak X represents the emission spectrum of the donor species and the peak Y represents the absorption spectrum of the acceptor. Figure 3b shows the emission spectrum Z of donor in the presence of acceptor showing modified peak height ratio as a result of differential quenching of the independent transitions. The ratio between emissions in the component peaks can be used to determine analyte binding, much as wavelength ratio measurements with fluorescent ion-sensitive dyes are used to quantify ion concentrations in biology. These measurements are advantageous when the energy acceptor acts simply to quench emission from the donor, and is not itself luminescent. Ratio measurements are independent of the amount of the donor species, but depend on the relative amounts of free and acceptor-bound species. Such ratio measurements can be conducted without time resolution, but are compatible with time-resolved measurements or equivalent frequency domain measurements for enhanced background rejection. Where the upconversion medium does not have appropriate independent transitions, it may be possible to introduce a suitable reference dopant (which need not necessarily be excited by multiphoton means, though would advantageously have long-lived luminescence). Reference is now made to Fig. 4 which schematically illustrates assays in accordance with the second preferred embodiment of the invention. Fig. 4a illustrates an assay in which the analyte A causes cleavage of a bond linking a quenching species Q to a bound luminescent (e.g. fluorescent) label F. When Q is cleaved from F by the action of the analyte the luminescent label F is able to fluoresce as a result of energy transfer from the excited donor. The excitation condition of D or F may be monitored to determine the analyte. Fig. 4b illustrates an assay in which a pro-luminophore, e.g. a pro-fluorophore (PF) is converted to a luminescent species by the action of the analyte A. The pro- luminophore is attached to the surface of the up-conversion medium. The action of the analyte results in the generation of a luminescent substance able to accept energy from the excited up-conversion medium. In this case the emission of the luminescent product is sensitised by the up-conversion medium and has the same time course as the decay of the excited medium. Thus, for example, in the event that a short lived luminescent species is bound to the surface of an energy donor of long excited state lifetime, then the sensitised luminescence of the acceptor will also have a long decay time and can be detected by time gated methods or equivalent as discussed previously. Such lifetime-resolved detection has been used previously in luminescence assays using conventionally excited donors. The present invention has the advantage that background luminescence will not be excited efficiently by the excitation source used to activate the up-conversion medium. Fig. 4c illustrates an assay in which a colourless species, e.g. a leuco-dye (LD) is bound to the solid phase of the up-conversion medium and is converted to a coloured species by the action of the analyte A with associated quenching of donor emission. All of the assays shown in Figs. 4a-c may be effected in "reverse". Therefore, for example, the assay of Fig. 4b may be operated in a manner such that the analyte converts a fluorescent species to a non-fluorescent species. In the case of Fig. 4c the assay may be effected such that the analyte converts a coloured species to a non- coloured (or differently coloured) species. A ligase or similar enzyme might catalyse bond formation rather than cleavage in the example of Fig. 4a. A configuration for the excitation of luminescence by a single light source 1 is shown in Figure 5. A waveform generator 2 provides a modulation signal with at least two frequency components to the light source 1. Which is focused to a beam by a lens 3 and unwanted wavelengths are rejected by an optical filter 4. Focused light is incident on sample 5 and luminescence from the sample is collected by lens 6, optically filtered by filter 7 and detected by detector 8 which is an electronic device such as a photomultiplier or semiconductor detector. The detected signal is amplified and optionally filtered by a signal-conditioning unit 9 and is furnished to optional frequency selective elements 10 and 11. The outputs of these elements, or of unit 9 directly, are fed into a control system 13 which is a computational device able to analyse the frequency distribution of the signal(s) and optionally to control the operation of the electronic drive means (unit 2). Although the description in relation to Fig. 5 is directed to the use of a single light source, it is to be understood that light source 7c could be replaced by individually controlled, multiple sources. If the luminescence decay time of a species sample is long, it is particularly convenient to excite the sample with a source or sources whose individual modulation frequencies are sufficiently high that emission will be unable to reproduce fluctuations at the or each modulation frequency or at higher frequencies. In this case, a difference frequency can be chosen to be low, so that the only component of the detected signal which will fluctuate significantly is at the difference frequency (F1-F2). This allows discrimination against any short-lived background which might be excited by two- photon or multiphoton processes. Any signal detected at the sum frequency F1+F2 will originate from contaminants of short lifetime which are able to reproduce high frequency fluctuations of emission, while the component at the difference frequency will be the sum of the desired emission from the long-lived component and that of the contaminant. There are other detection schemes that can be implemented to optimise discrimination between the different signals characteristic of label, background and scattered light and these will be familiar to those skilled in the art of signal processing. Although the above is set out in terms of light sources modulated sinusoidally, it is equally applicable to other modulation schemes. For example, if the signals are modulated as patterns, pulses or square waves, then a desired signal will be recoverable by appropriate manipulation of a resulting detected luminescence. In a simple case where an excitation requires two different wavelengths but where neither wavelength alone is able to excite the species (e.g. a sequential process proceeding via an intermediate state), excitation is a logical AND of the modulation patterns of the two wavelengths. Unwanted signals can be detected and subtracted by simple logical manipulations of the detected emission gated by the modulation patterns driving the light sources. These alternative modulation schemes are useful insofar as they exploit the multiplicative nature of two-photon or multiphoton processes to achieve detection of luminescence with minimal interference. Possible modulation schemes other than simple modulation of intensity at fixed frequencies (such as frequency or phase modulation of the intensity-modulating signal) may also be used. Detection at harmonics of the modulation wave form is appropriate to upconversion media excited by multiphoton absorption and is particularly useful where relatively high power density is needed for efficient excitation. Under these conditions, it is quite possible that Anti-Stokes fluorescence or upconversion excitation of background luminescence could be seen as an interfering signal. Long- lived species such as lanthanide chelates and cryptates and complexes of, for example, ruthenium osmium, rhenium etc. are potentially useful as labels excited by multiphoton means and have long-lived emission relative to most sources of background but require high power density for efficient excitation. Labels such as these are well suited to the proposed excitation and detection schemes. Such labels typically either have strong charge-transfer absoφtion bands between metal and ligand or utilise efficient intramolecular energy transfer from organic ligands to the bound metal ion.

Claims: CLAIMS 1. A method of performing an energy transfer luminescence assay to determine an analyte in which a donor species is irradiated to raise the donor species to an excited state, and the analyte causes a change in the excitation condition of at least one of the donor species and an acceptor species and said change is monitored to determine the analyte wherein (i) the donor species is provided as, in or adsorbed to a solid phase and is an upconversion medium capable of effecting, by energy pooling or otherwise, an energy transition to an excited state by absoφtion of electromagnetic radiation having an energy less than that of said transition, (ii) the acceptor species is bound or is capable of being bound directly or indirectly to the surface of said solid phase, (iii) the donor species is irradiated with said electromagnetic radiation, and (iv) luminescence is detected in at least one spectral region characteristic of the emission of the donor species and/or the acceptor species provided that the excitation of the acceptor species to an excited state capable of luminescence in a defined spectral region of the acceptor species does not occur by absoφtion of a single quantum of the radiation used to excite the donor species. 2. A method as claimed in claim 1 wherein the transfer of energy from the donor to the acceptor is detected by decrease in luminescence efficiency of the donor. 3. A method as claimed in claim 1 wherein the acceptor is capable of luminescence subsequent to the said transfer of energy and wherein the luminescence of the acceptor is used to detect the bound species. 4. A method as claimed in any one of claims 1 to 3 where the up-conversion medium is excited by simultaneous absoφtion of two or more photons of the same or different energy. 5. A method as claimed in any one of claims 1 to 3 where the up-conversion medium is excited by sequential absoφtion of two or more photons of the same or different energy. 6. A method as claimed in any one of claims 1 to 5 wherein the medium is an upconversion phosphor designed to absorb long wavelength radiation and to emit light at shorter wavelength. 7. A method as claimed in any one of claims 1 to 6 wherein the up-conversion medium requires a priming dose of energy to excite species therein to metastable levels which are required for up-conversion processes to be possible. 8. A method as claimed in claim 7 wherein the up-conversion medium is an electron-trapping phosphor. 9. A method as claimed in claim 1 wherein the up-conversion medium relies on excitation of an organic molecule to an excited state which subsequently relaxes to a metastable level of longer lifetime than the original state and wherein absoφtion of a second photon promotes re-excitation of the metastable state to another state of higher energy which is capable of luminescence or transfer of energy to an acceptor, or of intersystem crossing giving rise to an excited state of different spin multiplicity that is itself capable of luminescence or transfer of energy to an acceptor. 10. A method as claimed in claim 9 wherein the metastable state is the triplet state of an organic molecule. 1 1. A method as claimed in any one of claims 1 to 6 wherein the up-conversion process is based on the excitation of lanthanide ions in an appropriate matrix. 12. A method as claimed in any one of claims 1 to 6 wherein the exciting radiation is absorbed by one or more species within the medium and subsequently transferred to one or more other species within the medium resulting in excitation of the said other species which provides the donor species. 13. A method as claimed in claim 12 wherein the primary absorbing species is the ytterbium ion. 14. A method as claimed in claim 12 or 13 wherein the absorbed energy is ultimately transferred within the medium to ions of erbium thulium or other luminescent lanthanide which provides the donor species. 15. A method as claimed in any one of claims 1 to 6 wherein the excited species is able to delocalise its excitation by internal transfer of energy between similar sites within the medium. 16. A method as claimed in claim 15 such that delocalisation of excitation results in transfer of energy from sites within the bulk of the medium to sites at or near the surface of the medium which are subsequently able to transfer energy to the bound species. 17. A method as claimed in claim 1 wherein the solid phase is a glassy matrix, e.g. as produced by a sol-gel process. 18. A method as claimed in claim 1 wherein the solid phase is a crystalline matrix. 19. A method as claimed in claim 1 wherein the solid phase is an organic or inorganic polymer. 20. A method as claimed in any one of claims 1 to 19 wherein the analyte causes a change in the extent of binding between the solid phase of the donor species and the acceptor species. 21. A method as claimed in claim 20 wherein the assay is conducted in a format in which the analyte causes an increase in the degree of binding of the acceptor to the donor. 22. A method as claimed in claim 20 wherein the action of an enzyme or other catalytic species is monitored by activation of coupling or uncoupling between donor and acceptor either by catalysing formation or cleavage of a linkage between them or by unmasking of protected groups on either or both of the donor and acceptor species, resulting in formation or dissociation of a complex between the said species or formation or cleavage of a chemical bond between them. 23. A method as claimed in claim 20 wherein the assay is conducted in a format in which the analyte causes a decrease in binding of the acceptor to the donor. 24. A method as claimed in claim 20 wherein the surface of the solid phase is so modified as to facilitate binding of the analyte and/or to protect the solid phase from interaction with or dissolution in an aqueous medium. 25. A method as claimed in claim 24 wherein the surface of the solid phase has antibodies, lectins oligonucleotides or other recognition ligands bound to it. 26. A method as claimed in any one of claims 1 to 19 wherein the analyte is one which affects the acceptance properties (for energy transfer from the donor species) of a moiety bound to the solid phase of the donor species, and/or which affects the ability of the bound moiety to emit luminescence consequent on excitation by said energy transfer. 27. A method as claimed in claim 26 wherein the assay is used to monitor the formation or cleavage of a bond linking a quenching species or an enhancer of emission to the solid phase or to a luminescent moiety bound thereto. 28. A method as claimed in claim 26 wherein the moiety is one which is converted by the analyte to a luminescent species capable of accepting energy from the excited donor species. 29. A method as claimed in claim 26 wherein the moiety is a pre-existing luminescent label whereof luminescence is quenched by the action of the analyte. 30. A method as claimed in claim 26 wherein the moiety is one which is able to change colour as a consequence of the action of the analyte. 31. A method as in any one of claims 1 to 30 wherein the detector is a semiconductor diode. 32. A method as claimed in any one of claims 1 to 30 wherein the detector is a linear charge-coupled device or MOS sensor. 33. A method as claimed in any one of claims 1 to 30 wherein the detector is an imaging detector. 34. A method as claimed in claim 33 wherein the detector uses an image intensifier or imaging single photon detector. 35. A method as claimed in claim 33 or 34 wherein the detector is a CCD camera, or other solid state imaging device. 36. A method as claimed in any one of claims 1 to 35 wherein the source of long wavelength radiation is a semiconductor light-emitting diode or laser. 37. A method as claimed in any one of claims 1 to 36 wherein the exciting radiation is pulsed or modulated in intensity. 38. A method as claimed in claim 37 wherein gated detection is used to confine detected signals to one or more defined time periods relative to the excitation. 39. A method as claimed in claim 37 wherein the detection system uses a periodically switched, gated or modulated detector to implement lock-in detection or similar correlated detection schemes. 40. A method as claimed in claim 37 wherein the detector is an imaging detector which is gated or modulated in sensitivity. 41. A method as claimed in claim 37 wherein the detector is designed to implement phase-sensitive detection in conjunction with a periodic excitation source. 42. A method as claimed in any one of claims 37 to 41 wherein the detector is an interline CCD camera equipped with a charge drain capable of periodically resetting the camera, but wherein charge can be preserved and integrated by transfer to storage registers prior to the drain operation periods for subsequent readout at a later time. 43. A method as claimed in any one of claims 37 to 41 wherein the detector is a camera equipped with a fast optical shutter. 44. A method as claimed in claim 1 wherein the observed region of the sample for analysis is illuminated by one or more focused excitation sources of long wavelength photons, such that the efficiency of excitation of the up-conversion medium is markedly greater at or near the focal plane than otherwise as a consequence of a nonlinear relationship between photon density and excitation efficiency. 45. A method as claimed in claim 44 wherein two excitation sources of different wavelength are used and the excitation efficiency is proportional to the product of the first or higher powers of the photon densities of each individual excitation source. 46. A method as claimed in any one of claims 1 to 45 wherein the up-conversion medium gives rise to two or more luminescence emission peaks which are differently affected by binding of an energy acceptor and where measurements of emission at two or more wavelengths characteristic of these peaks are combined by ratio or otherwise to effect a measurement of the analyte. 47. A method of detection of luminescence in which a luminescent species of interest is excited by a process of simultaneous or sequential absoφtion of at least two photons from at least one photon source, wherein the or each photon source is modulated at one or more frequencies, and luminescence measurements are made at a frequency or frequencies different from the or each modulation frequency, the or each frequency at which measurements are made being a function of the or each modulation frequency by virtue of the excitation process. 48. A method according to claim 47, wherein the species is excited by photons from a single source which is modulated at a fixed frequency, and measurements are made at a frequency which is a harmonic of the fixed frequency. 49. A method according to claim 47, wherein the species is excited by photons from at least two sources which are modulated at different frequencies, and measurements are made at a frequency which is a function of a linear combination of the different frequencies. 50. A method according to claim 47, wherein the species is excited by photons from a single source which is modulated with a composite waveform comprising the sum of two or more different frequencies, and measurements are made at a frequency which is a function of a linear combination of the different frequencies. 51. A method according to claim 49 or 50 wherein the species of interest has a luminescence decay time and the frequencies are selected such that emissions excited by the or each source are not able to reproduce the modulation frequencies but are able to reproduce frequency components corresponding to the difference between the or at least two of the modulation frequencies, and measurements are made at the frequency of said components. 52. A method according to claim 51, wherein the presence of an interfering species capable of luminescence as the result of multiphotonic excitation processes, but with a shorter luminescence lifetime than the species of interest, is detected by measurement at one or more frequencies selected such that a signal from the species of interest will suffer substantial attenuation by virtue of demodulation while a signal from the interfering species will not suffer substantial attenuation. 53. A method according to claim 52 wherein measurements are made at frequencies that are the sum and difference of the modulation frequencies and, a function of the result of the measurement at the sum frequency is subtracted from a function of the result of the measurement at the difference frequency to suppress contributions from short-lived emissions. 54. A method according to any one of claims 47 to 53, wherein the luminescent species is a complex, cryptate or similar moiety comprising one or more organic molecules in close association with one or more metal ions, and wherein the emission originates from electronically excited states of one or more of the metal ions, either directly or by means of energy transfer to another species capable of luminescence. 55. A method according to claim 54, wherein the energy required to excite the metal ion species is transferred thereto from excited organic species in close proximity. 56. An apparatus for detecting luminescence in which a luminescent species is excited by a process of simultaneous or sequential absoφtion of at least two photons, comprising at least one photon source, means for modulating the or each photon source at a pre-determined frequency, means for detecting emissions at a frequency which is different from the or each modulation frequency but which is a function of the or each modulation frequency by virtue of the excitation process.

Inventors: MORGAN CHRISTOPHER GRANT, MORGAN, CHRISTOPHER, GRANT
Applicants: MORGAN CHRISTOPHER GRANT, PHOTONIC RESEARCH SYSTEMS LIMI, MORGAN, CHRISTOPHER, GRANT, PHOTONIC RESEARCH SYSTEMS LIMITED
Application year: 1998
Main classification: G01N21
IPC codes: G01N21, G01N33