SYNTHESIS OF LONG-WAVELENGTH ABSORBING PHOTOSENSITIZERS

Abstract: The present invention provides for novel therapeutic macrocycle compounds useful in photodynamic therapy that are based on the chlorin ring system. The macrocycle compounds have, in many cases, wavelengths of activation at about 670 nm, characteristic of chlorins, and are stabilized against oxidation by the attachment to the chlorin ring of a structure that comprises one or more exocyclic rings that contribute at least one nitrogen atom. Protonation or covalent modification of this nitrogen atom, or other covalent modification of the one or more exocyclic rings permits optimization of pharmacologically relevant properties including, for example, solubility. Representative chlorins include the pyridochlorins depicted by formulas (I), (II), (III).

Description: ® Synthesis of Long- Wavelength Absorbing Photosensitizers The present United States patent application claims priority under 35 USC section 119 of Canadian patent application 2,221,912 of Johnson et al., filed November 21. 1997, and entitled "Photosensitizers with Improved Biodistribution and Light- Absorbing Properties". The Canadian application, including the complete text and figures thereof, is incorporated by reference herein, as if fully set forth. Field of the Invention The present invention provides for novel therapeutic macrocycle compounds useful in photodynamic therapy that are based on the chlorin ring system. The macrocycle compounds have absorption spectra optimized for therapeutic use in tissues, and are stabilized against oxidation by the attachment to the chlorin ring of a structure that comprises one or more exocyclic rings that also contribute at least one nitrogen atom. Protonation or covalent modification of this nitrogen atom, or other covalent modification of the one or more exocyclic rings, permits optimization of pharmacologically relevant properties including, for example, solubility. Representative of such chlorins are the "pyridochlorins" depicted as follows. β-isomer γ-isomer Background of the Invention Photodynamic therapy ("PDT") generally involves administration of a drug (photosensitizer) which, when irradiated with light, becomes electronically activated 1 such that it may interact with molecular oxygen to generate reactive oxygen species. The reactive oxygen species are believed responsible for killing of targeted cells, which are generally those associated with an unwanted hyperproliferating state. Unless a photosensitizer compound is to be used solely for the treatment of superficial skin diseases, it is important that the wavelength(s) at which the compound absorb light be optimized. Preferably, the compound should absorb strongly in the red region of the spectrum (650-800 nm). Light scatteπng and the presence of endogenous chromophores (such as hemoglobin) results in very poor penetration of tissues by light at wavelengths below about 600 nm. This means that the large absorption band (the so called Soret band) displayed by porphyrins in the region of 400 nm is not available, practically speaking, for photosensitizer activation in PDT. Instead, the longer wavelength Q absorption bands must be used. However, the longest wavelength Q band ior Phototπn *> (see U S Patent No 5.059,619), a first generation porphyπn photosensitizer, is only at about 630 nm. Although this wavelength is long enough to permit useful photodynamic therapy approaches for some tumors, it is not ideal. Since light penetration of human tissues typically doubles between 630 and 750 nm, a photosensitizer absorbing at 750 nm would be far more effective at treating thick tumors than, for example. Photofrin 2 ) However, increasing the absorption wavelength of photosensitizer compounds beyond about 800 nm ( into the infrared), would not give rise to further improvements, since the involved energy transitions are insufficient to generate a sufficiently energetic excited state, and corresponding activated oxygen species. Accordingly, there is a considerable medical need to develop classes of photosensitizers having optimized light absorption properties. One such improved class of photosensitizers are the so called "green monohydrobenzoporphyrins" which are derived from natural porphyrins by Diels-Alder type reactions at one of the functional groups attached to the porphyrin core. An example of such a compound, which is currently in phase III clinical trials, is BPDMA which shows considerable absorption at about 688 nm. In this regard, see for example. U.S. Patents 5.095.030; 5,171.749; 5,776.966 and the like. Finally, the porphyπn core structure is characteπstically non-polar, and such structures need to be modified by the addition of groups having sufficient polaπtv to improve the solubihtv and amphiphi citv propeπies of the compound, and to improve the rate of metabolism or clearance in the bodv The core structure known itself as porphynn. as mentioned above, is presented below in comparison with that of chlorin In principle, the simplest wav to increase the wavelength of absorption of a porphyπn would be bv reduction of a double bond therein to give the corresponding chlorin (reduction of a porphynn to a chlorin results in an increase in both the intensitv and wavelength of the longest absorption band - providing a shift of about 25 nm and typicallv a substantial increase in extinction coefficient) PYRROLE PORPHYRIN PORPHYR1N Although reducing agents such as dnmide are available that will regioselectively reduce only the double bond targeted for simply conversion of a porphyπn to a chloπn. such reactions are t\ pically reversible Oxygen in the air is well known to oxidize chlorins back to porphyrins and this impacts not onlv the synthesis, but also the storage and clinical use of the resultant compound Accordingly, there are only a limited numoer of cases where such procedures have been used to svnthesize chlorins (see for example R Bonnet, Chem Soc Rev . 1995 RECTIFIED SHEET (RULE 91) p. 19. and R. Bonnet et al.. Biochemical Journal. 261. p. 277, 1989, in relation, for example, to the synthesis of tetrakis (m-hydroxyphenyl) chlorin, " m-THPC"). Examples of known chlorins that contain unsaturated exocyclic rings fused to the skeleton between a meso position and its adjacent β-position include puφurins and benzochlorins. Fusion of such exocyclic rings has the advantage of substantially limiting oxidation of the prepared chlorin at the previously reduced pyrrole ring. A number of puφurins have been described. See, for example, R.B. Woodward et al.. J. Am. Chem. Sυc. 82. p. 3800, 1960. and also IH. Fuhrhop et al.. Angew. Chem. Int. Ed. Engl., 14, p. 361, 1975 describing an octaethyl puφurin which absorbs at 695 nm. A number of puφurins have been shown to have photodynamic activity, and the most effective member of this class of compounds may be a tin etiopuφurin formed by cyclization of τne5o-[β-(2-ethoxycarbonyl)vinyl] etiopoφhyrin. followed by metallation with tin chloride. This compound is currently in clinical trials (A.R. Morgan et al., Photochem. PhotobioL, 51, p. 589, 1990; A.R. Morgan et al.. J. Med. Chem.. 32. p. 904, 1989). The synthesis of a benzochlorin was first described by D.P. Arnold et al. J.C.S. Perkin I, p.1660, 1978. and sulfonation of a benzo ring thereof was effected in concentrated sulfuric acid (see A.R. Morgan et al., Photochem. PhotobioL 55. p.133, 1992 and B.C. Robinson et al.. SPIE Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V, 2675, p. 179. 1996). The resulting sulfonic acid group can be used as a platform for further derivatization. such as to modify bioactivity. An additional pathway to effect derivatization of a benzochlorin involves reaction of a metallated benzochlorin at the meso position adjacent to a gem-diethyl group to yield an iminium salt (D. Skalkos et al.. Photochem. PhotobioL ,59, p. 175 1994). Photodynamic activity has been demonstrated for various benzochlorins (see A.R. Morgan et al., Photochem. PhotobioL 55. p.133, 1992, and A.R. Morgan et al.. Tetrahedron Letters. 35, p. 5347. 1994). The preparation of a pyridinopoφhyrin has been described. C. Alonso et al.. Tetrahedron Letters. 38(15), pp. 2757-2758, 1997 wherein the pyrindinyl nitrogen atom, or the pyridinyl ring, may serve as a platform for further derivatization. However, such a compound is believed to lack the optimized absoφtion profile characteristic of the pharmaceutically useful chlorins. As aforementioned, the presence of an exocyclic ring fused to the core structure of a chlorin at the site of the reduced pyrrole ring substantially prevents re- oxidation thereof. It would be further advantageous to derivatize the exocyclic ring to optimize biological properties such as solubility, physiological clearance, or to enhance amphiphilicity, that is the presence of both polar and non-polar domains thus enhancing interaction with both polar and non-polar environments. Although derivatization has been described, for example, for benzochlorin by sulfonation under strongiy acidic conditions, there is a clear need to develope more flexible methods whereby a large number of such pharmaceutically useful chlorins can be prepared. As described below, the present invention provides such methodology and resultant compounds. Summary of the Invention The present invention provides for novel therapeutic macrocycle compounds useful in photodynamic therapy that are based on the chlorin ring system. The macrocycle compounds are stabilized against oxidation by the attachment to the chlorin ring of a structure that comprises one or more exocyclic rings that contribute at least one nitrogen atom. Protonation or covalent modification of this nitrogen atom, or other covalent modification of the one or more exocyclic rings (hereinafter "derivatization "" ) permits optimization of pharmacologically relevant properties including, for example, solubility. Optionally, the chlorin ring is metallated. In a preferred embodiment of the invention, compounds are represented by the formula (1) (1 ) wherein, for example. R la , R l b are independently H or alkyl; R 2 is OH, halogen, alkoxy, OCO-alkyl. sulfonate. sulfate. or phosphate; R 3a is H. or a phenyl or other aryl or heteroaryl group optionally substituted by one or more groups, each independently selected, for example, from halogen, hydroxy. alkyl, alkoxy, cyano, and ester; R 3b is H, halogen, formyl, nitro. amino or cyano R 4 is H or alkyl; M is a complexing metal, typically known in the art, or represents 2H; and N. the nitrogen atom of the pyridine ring, is optionally in the form of an N-oxide. or a salt such as an alkyl or hydrogen halide. In a further preferred embodiment of the invention, compounds are represented by the formula (2) wherein the substituents are generally as for compound ( 1 ) although certain preferences or exceptions are described as follows: R la is not H. and is preferably methyl or ethyl, in which case R lc (formed from an R la ) would be CH 3 CH= or CH2= ; R lb is as for compound (1); R 2 is H or alkyl (and fixed at the position alpha to the N atom); R 3a is as for compound (1 ); R is as for compound ( 1 ); The C=0 group may, optionally, be converted to a CHOH group, and the resultant OH group may be further derivatized. such as via the R" options described for compound (1 ); It should be noted that since the exocyclic ring is not aromatic ( comparing to compound 1 ), the N-oxide or salt variations are inapplicable, although compound (2) can exist as a free base or a metal complex. In an additional embodiment of the invention, compounds are represented by the formula (3) wherein, preferably, R ia would be methyl or ethyl: R l b would be as for compound ( 1 ): R 3a would be as for compound ( 1 ); R 3b would be as for compound ( 1 ); and either R lc , together with the carbon atom to which it is bonded, forms a group and R 2a and R 2b together form a bond; or R lc is CH 3 CH or CH 2 ; R ,'2a a is H: and R 2b is R lb . With respect to the design of such compounds(3), the N-oxide or salts mentioned for compound (1) are also within the practice of the invention, and compound (3) can also exist in free base or as a dimetai complex. As aforementioned, more than one exocyclic ring may be present in the compounds of the invention, there being generally no structural limitation on any such combination of rings subject of course to practical considerations of synthesis, and that one or more suitable nitrogen atoms be appropriately placed therein. Accordingly, additional representative compounds of the invention include: RECTIFIED SHEET (RULE 91) 10 RECTIFIED SHEET (RULE 91) and the like, where it is additionally understood that more than one nitrogen atom may be present in the exocylic rings, which may be further substituted by other functional groups (such as hydroxyl or keto) and which permit further enhancements in desireable properties such as solubility. Additional preferred compounds include the "pyπdochlorins " such as: (i-ιsomer Additional preferred examples of the invention include: 1 1 RECTIFI ET RULE 91 Brief Description of the Drawings Figure 1 depicts a synthesis ot octethylbenzochloπn Figure 2 depicts a vinylogous Vilsmeier reaction of metalled octaethylpoφhyπn Figure 3 depicts a possible synthesis of the u-imino precursor, species (92). Figure 4 depicts the product expected from the cychzation of species (92). Figure 5 depicts the synthesis of puφuπns. Figure 6 depicts the synthesis of australaochloπns. Figure 7 depicts a postulated mechanism for the removal of the angular ethyl group of species (93) based an analogous reaction known for steroid ring A Figure 8 depicts a possible cyclization reaction for glycolaldehyde condensation product, species (96). Figure 9 depicts the structure of amines (98) and (99), synthesis of which was unsuccessful. Figure 10 depicts a methylcarbamate condensation of (56) with subsequent cyclization. Figure 1 1 depicts a proposed route to /we.rø-amιnomethyloctaethylpoφhyrin ( 104). Figure 12 depicts a proposed use of (105) as a cychzation intermediate. Figure 13 depicts the synthesis of Compound 106. Figure 14 depicts a side-product from N-hydoxymethylation of (Ni- 105, mckelated 105). Figure 15 depicts a proposed cyclization of N-formylamide compound (109). Figure 16 depicts a synthesis of meso-isocyanooctaethylpoφhyrin (1 12). Figure 17 depicts a Passerini reaction of (1 12). 12 RE RULE 91 Figure 18 depicts acid-cataiyzed reaction of (1 12) with formaldehyde to give ( 116). Figure 19 depicts a rationale for the cyclization lack of cyclization of the two types of hydroxymethylamide (106) and (1 16). Figure 20 depicts a possible mechanism for the formation of the dimeric structure (Ni- 1 19). Figure 21 depicts a mechanism for the formation of the dimer (Ni-120). Detailed Description of the Invention The present invention provides for novel therapeutic macrocycle compounds useful in photodynamic therapy that are based on the chlorin ring system. The macrocycle compounds of the invention have relatively long wavelengths of absoφtion. to permit penetration of tissues, such as about 670 nm. although this may vary with the particular compound based upon its exact cyclic structure and ring substituents. The compounds are stabilized against oxidations, such as at the reduced ring of the chlorin (which would cause the molecule to revert to a poφhyrin structure), by the presence of one or more exocyclic rings in contact with the reduced pyrrole ring. In a typical embodiment, the exocyclic ring or rings are aromatic, although they may also be non-aromatic or have partial aromatic character. Most preferably the exocyclic ring or rings comprise at least one nitrogen atom, or comprise at least one substituted carbon atom, or a carbon atom which is subject to substitution. As a result of this modification to the exocyclic ring(s), and/or further derivatization at such sites, physiologically relevant properties of the compound can be improved, such as improved solubility, amphiphilicity, drug clearance, and the like. Accordingly, there are provided compounds according to the structures 13 RECTIFIED RULE 91 wherein the circle represents an exocyclic structure having one to about three rings and preferably at least one nitrogen atom which can be protonated or further substituted. R ' represents one or more substituents in the πng structure which may be selected (see below) to further enhance or modify the compound's properties. As depicted directly above, the exocyclic structure can be positioned on either side of the reduced pyrrole πng of the chlorin. Such positions could be considered as symmetrical, except for the asymmetries introduced into the compounds by the other substituents ( see also below) Most preferably, the exocyclic structure contains from one to about three rings, and from about 5 to about 25 carbon atoms, wherein preferably at least some of the carbon atoms are involved in double bonds. It is particularly preferred that the exocyclic structure contain one or more ring nitrogen atoms which, typically, in derivatized or protonated form, can be used to enhance the solubility in aqueous systems of the macrocycle. The exocyclic structure is subject to further modification by from one to many groups R ' . which similarly to the other R groups attached to the chlorin πng itself, may be used to modify the properties of the macrocycle. Options 14 R RULE 91 for selection of groups R ' are generally the same as for other R groups present in the structure, although there would be exceptions as is readily understood in the art. Accordingly, representative exocyclic structures useful in the practice of the invention include those depicted below: 15 1 16 RECTIFIED SHEET (RULE 91) In a preferred embodiment of the invention, compounds are represented by the formula ( 1 ) (1) wherein, for example, R la . R lb are independently H or alkyl; R 2 is OH. halogen, alkoxy, OCO-alkyl. sulfonate. sulfate, or phosphate; R 3a is H, or a phenyl or other aryl or heteroaryl group optionally substituted by one or more groups, each independently selected, for example, from halogen, hydroxy. alkyl, alkoxy, cyano. and ester; R is H, halogen, formyl, nitro, amino or cyano; R 4 is H or alkyl; M is a poφhyrin-complexing metal, typically known in the art. or represents 2H: and N, the nitrogen atom of the pyridine ring, is optionally in the form of an N-oxide. or a salt such as an alkyl or hydrogen halide. It will be appreciated that the exact position of the nitrogen atom in the exocyclic structure is subject to considerable variation, limited of course by available 17 RE T RULE 91 routes of synthesis, and thus numerous other compounds are preferred according to the practice of the invention. Representative compounds include β-ιsomer γ-isomer In connection with the design of such compounds, additional substituents may be used as long as they do not interfere with the intended therapeutic activity, or capacity of the compound to be formulated for use. As will be appreciated by those skilled in the art. additional derivatizing groups may be used, such as other charged or polar groups, to affect, for example, compound solubility, the range of such substituents being limited of course, by the feasability of available synthesis routes. The addition of further functional groups to the exocyclic structure provides additional opportunities for derivatization and fine tuning of pharmacologically relevant properties. In a further prefeπed embodiment of the invention, compounds are represented by the formula (2). wherein the substituents are generally as for compound ( 1 ) although certain preferences or exceptions are described as follows: 18 R la is not H. and is preferably methyl or ethyl, in which case R lc (formed from an R la ) would be CH 3 CH= or CH2= ; R lb is as for compound (1); R 2 is H or alkyl (and fixed at the position alpha to the N atom); R a is as for compound (1 ); R 3b is as for compound ( 1); The C=0 group may. optionally, be converted to a CHOH group, and the resultant OH group may be further derivatized. such as via the R 2 options described for compound (1). It should be noted that since the depicted exocyclic ring is not aromatic (comparing to compound 1 ). the N-oxide or salt variations are inapplicable, although compound (2) can exist as a free base or a metal complex. As aforementioned, the exact position of the nitrogen atom and any further functional groups in the exocyclic structure can be varied considerably. Accordingly, representative related compounds of the invention include 19 In an additional embodiment of the invention, compounds are represented by the formula (3) wherein, preferably, R la would be methyl or ethyl, R lb would be as for compound ( 1 ), R 3a would be as for compound ( 1 ), R 3b would be as for compound ( 1 ), and either l a τ l b R , together with the carbon atom to which it is bonded, forms a C(R )(R ) group and R 2a and R 2b together form a bond: or R lc ιs CH 3 CH or CH 2 , R 2a is H, and R 2b is R lb . With respect to the design of such compounds(3), the N-oxide or salts mentioned for compound (1 ) are also within the practice of the invention, and compound (3) can also exist in free base or as a dimetal complex. It is again noted that generally the compounds of the present invention can exist in a metallated form, or the metal atom of the chlorin πng(s) can be replaced by 2H. as would be readily apparent to those skilled in the art. 20 RECTIFIED SHEET RULE 91 Pharmaceutical Administration The compounds of the invention may be formulated in a variety of ways for pharmaceutical use. Generally speaking, such formulations include any excipients, stabilizers, emulsifying agents, osmotic agents, solubilizing agents and the like, that are recognized as useful to deliver photosensitizer compounds to the body whether topically or internally in any way such as by intravenous, intraperitoneal. or intramuscular injection, transmucosally, oraily, transdermally by way of skin patches, salves and gels, and such. Liposomal formulations, as recognized in the art, represent a preferred form of formulation, including formulations with phosphatidyl serine, phosphatidyl glycerol, phosphatidyl choline, and the like. Additionally, it will be recognized that functional groups are typically added to the macrocycles of the present invention to facilitate their storage, preparation, solubility, and physiological utility. Generally, reference may be made to Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton, PA. latest addition. Additional information concerning generally acceptable formulations is provided in U.S. Patents 5.095.030; 5,171.749; 5,776,966; 5,789,433; 4,512, 762; 4,566.636: 5,399,583; 4,920. 143. pertaining to photosensitizers for pharmaceutical use. The compounds of the invention may generally be used for all of the therapeutic applications for which photosensitizer compounds have been recognized, as mentioned for example in the cited patents. As is recognized by medical practitioners, dosages vary considerably based on the mode of administration, formulation, condition of the patient, condition to be treated, and the like. For systemic administration, dosages on the order of 10 microgram/kg to 100 mg/kg, preferably 100 microgram/kg to 10 mg//kg may be preferred. With respect to topical administrations, suitable compositions may range from about 1 to 10% of the composition, or greater or lesser, depending upon the application, as would be recognized in the art. Examples Svnthesis Routes for Pvridochlorins 21 RECTIFIED SHEET (RULE 91) Although octaethylbenzochlorin (Arnold et al.. 1978) proved resistant to many kinds of derivatization reactions, its overall photochemical properties and stability suggested the desireability of synthesizing novel macrocylces that incoφorated some of its features. By substituting an exocyclic pyridine ring for an exocyclic benzene ring, a compound would be provided that would permit nucleophilic substitution reactions on the exocyclic pyridine ring, in contrast to mostly unsuccessful electrophilic substitutions attempted on the benzochlorin benzene ring. Representative of such "pyridochlorins " are the α-, β-, and γ- isomers of "octaethylpyridochlorin". as depicted below. β-ιsomer γ-isomer For demonstration puφoses, an evaluation was made as to which of the three isomers was likely to be the most easily synthesized. Here a comparison with the synthesis of octaethylbenzochlorin was made The formation of the latter is achieved by a vinylogous Vilsmeier reaction on metallated octaethylpoφhyrin to give the acrolein-substituted metallopoφhyrin (M-16 in Figure 1. Formation of Octaethylbenzochlorin). If a similar method were to be used for the synthesis of pyridochlorins, it would seem logical first to make the nitrogen analogues of the meso-acrolein. This reaction pathway would rule out the synthesis of the γ-pyridochlorin as this would require nucleophilic attack by the β-β double bond on the nitrogen atom, a highly unlikely reaction. For this reason, efforts were concentrated on synthesizing the α- and β-pyridochlorins. Example 1 The design and svnthesis of α-pyridochlorin 22 RECTIFIED SHEET RULE 91 In order to synthesize the α-pyπdochloπn it was necessary first to make (92), the α-imino analogue of the acrolein derivative (16) used in the benzochloπn synthesis (Figure 1 ) (M-16. "M" meaning metallated) is formed in two steps by electrophi c attack on the poφhyπn meso-caibon by the vinylogous Vilsmeier reagent formed from 3-(dιmethylamιno) acrolein and phosphoryl chloride, followed by treatment with aqueous base to hydrolyze the lmimum salt to the aldehyde (Figure 2). Clearly, another approach was necessary to create the α-mtrogen analog One well-known method of obtaining a poφhyπn weso-substituted by a nitrogen functionality is nitration. The nitro compound can be reduced to the ammopoφhyπn, and such compounds are reported to react with aldehvdes to give Schiff s bases (A W Johnson et al J Chem Soc (C), p 794, 1966) Thus a reasonable route to the desired precursor from octaethylpoφhyπn would be to prepare the nitro deπvative (90), reduce it. and then form the Schiff s base with glvoxal (Figure 3) The nitration was earned out in an ice-cold mixture ot glacial acetic acid and concentrated nitric acid to give the mono-nitro compound (90) in 50 % yield The reaction mixture had to be monitored carefully, in order to minimize the formation of poly-nitro compounds The reduction of the nitro group to the amine was effected with stannous chloride dihydrate in concentrated hvdrochloπc acid and gave the desired product (91 ) in 85 % vield Thus tar. the svnthesis proceeded as described in the literature. (A W Johnson et al.. J Chem Soc (C), p 794, 1966, R Bonnett et al., J Org Chem., 30, p. 2791 , 1965, A.W Johnson et al., J Chem Soc. p 4303, 1965) The formation of Schiff s bases by condensing aminoetiopoφhyπn with benzaldehyde and amsaldehyde has been descnbed as occurnng in good yield (> 80 %). However, these reactions were performed using the aldehyde as solvent, while glyoxal tπmer dihydrate (the aldehyde necessary for this synthesis) is a solid Hence a solvent system in which both the aldehyde and the poφhyπn would have reasonable solubility was required A number of small-scale expeπments lead to the finding that a mixture of ethanol and THF was a suitable solvent, the ethanol dissolving the glyoxal tnmer when heated, and the THF dissolving the poφhynn Although the condensations reported with benzaldehyde and anisaldehvde occuπed at room 23 RECTIFIED SHEET (RULE 91) temperature, it was necessary to reflux the glyoxai/poφhyrin mixture overnight. This might well be accounted for by the lower concentrations of reagents resulting from the use of a solvent. The optimized yield of the reaction was 69 %. or 80 % based on recovered starting material. Unsuccessful attempts were made to drive the reaction to completion by the addition of drying agents, but it is likely that the amount of residual water in the THF/ethanol solvent far outweighed the small quantity of water produced in the condensation. In any case, the product (92) could be separated from the polar starting material by column chromatography, and the latter could then be reused. Before attempting this condensation, it had been anticipated that dimer formation, resulting from the reaction of an aminopoφhyrin with each of the two formyl groups of glyoxal. might occur. However, the use of a large excess of glyoxal prevented the dimeric product from being formed in any significant quantity. (92) was stable enough to allow it to be chromatographed on silica, although care was taken to elute it from the column as quickly as possible as some decomposition to the amine was observed during chromatography. However, as it appeared to be sensitive to acid, and the cyclization step was expected to require the use of such conditions, problems with this step of the synthesis were envisaged. Consequently, attempts were made to reduce the imine functionality to the amine using sodium cyanoborohydride. a reagent reported to reduce imines selectively in the presence of carbonyl groups. Unfortunately, any products formed in this reaction appeared to be very unstable, and decomposed during work-up to give the amine (91 ). In view of this result, the cyclization reaction was performed directly on (92), in the hope that conditions favoring cyclization over hydrolysis could be developed. In an effort to minimize the possibility of hydrolysis, initial experiments employed nonacidic conditions, namely refluxing in toluene. Indications that neutral, high temperature conditions might be successful came during a synthesis of the imine. when the solvent inadvertently was allowed to boil away, and the reaction mixture was heated as a solid for several hours. On working up this product, no trace of the desired imine (92) was present; instead, in addition to the amine starting material (91 ), which was the major compound isolated, a few milligrams of a mixture of two low 24 RECTIFIED SHEET RULE 91 polaπty green compounds (93) was obtained. By 'H NMR and visible spectroscopy these compounds appeared to be two isomers with a chlorin structure. (93) On refluxing the imine in toluene overnight this result was reproduced, the isomeπc mixture (93) being formed in 15 % yield. No starting material was recovered, but a substantial quantity of the amine (91 ) was isolated; it seems that the cyc zation and hydrolysis reactions are in competition with one another, which would suggest that obtaining the cychzed product in high yield might not be possible Although the quantity of cyclic product (93) thus far obtained was small, and it consisted of a mixture of two isomers, it was relatively simple to determine the structure using spectroscopic techniques: firstly, the visible spectrum showed an absoφtion peak at 722 nm ( e = 10800), indicative of a reduced porphyπn species such as a chloπn (the longest- wavelength absoφtion of the precursor imine was at 660 nm and very weak (e = 4400)). Secondly, in the 40.0 MHz Η NMR spectrum there were some very distinctive peaks: two quartets at 7.45 ppm and 7 72 ppm and two doublets at 2.62 ppm and 2.81 ppm. These resonances are typical of the ethylidene functionality CHCH 3 attached via a double bond to the β-position of a reduced pynole ring. There were also two singlets at 7 98 ppm and 8.15 ppm. in addition to the expected meso- ydτogen signals, and these could be assigned to the imino CH of the two isomers Finally there were two tπplets at 0.10 ppm and 0.50 ppm. suggesting the presence of ethyl groups lying out of the plane of the macrocycle and hence expenencing a greater degree of shielding by the πng cunent than those lying in the plane. The two isomers (93) were present in a ratio of approximately 4 to 25 RECTIFIED SHEET RULE 91) 1. This information lead to the two isomers (93) being assigned the structures shown above. The formation of these products (93) from the cyclization reaction was somewhat suφrising. as it requires a formal oxidation. The expected product, given that the desired 1.2-dialkyl shift did not take place, and the carbocation created by the cyclization process was quenched instead by the loss of a proton from the attached β- ethyl group, would be the imino-alcohol (94) (Figure 4). In (93), the carbonyl group of the ketone is in conjugation with the imine double bond, which may increase its stability with respect to the alcohol, but the mechanism by which this transformation occurs is unclear. However, a similar oxidation is seen during puφurin synthesis (see below) when the reaction mixture is refluxed open to the air; in that case oxygen is presumed to be the oxidant. Despite the very close analogy to the octaethylbenzochlorin synthesis, the replacement of a CH group with a nitrogen atom obviously changes the cyclization mechanism significantly, even though the N atom is several bonds removed from the site of reaction. There appears to be a closer similarity to puφurin synthesis. These compounds are produced by the acid-catalyzed cyclization of (metal-free) meso- acrylic esters (Figure 5 A). There are two types of puφurins, named type A and type B. Type A purpurins possess an sp ' carbon atom on the reduced pyπole ring, adjacent to the exocyclic ring, while type B puφurins have an sp " carbon at this position (Figure 5B). The formation of type B puφurins requires a formal oxidation after cyclization. In some cases, the type A compound is produced when the cyclization is performed under nitrogen, while the type B arises when this step is performed in a vessel open to the air. However, this is not always true, and the outcome varies according to the particular system. Studies are on-going in this area to elucidate the mechanism controlling the formation of the two types of puφurin (for background, see K.M. Smith et al, J. Org. Chem.. 48, p.500. 1983 and A.R. Morgan et al., J. Org. Chem.. 51. p. 1347, 1986) The cyclization of the imine displays characteristics of a 26 RECTIFIED SHEET (RULE 91) type B puφurin synthesis, although the product contains a 6-membered exocyclic ring, reminiscent of a benzochlorin preparation. Very recently a report of a system bearing similarity to our own appeared, describing the synthesis of a new class of chlorins possessing 6-membered exocyclic rings, the "australochlorins " ' (D. Yashunsky et al.. Aust. J. Chem.. 50, p. 487, 1997). These compounds are isomeric with the benzochlorins but have a β-ethylidene group adjacent to the fused ring, which is non-aromatic. They are obtained by the thermolysis of the trimethylammonium salt as a mixture of two geometric isomers in a ratio of 10:7 (Figure 6). Spectroscopically, these compounds are typical metallochlorins, absorbing at 643 nm, and hence are less interesting as potential photosensitizers than (93). which has a significantly red-shifted spectrum. Although the cyclization experiments performed to this point had been successful in producing quantities of the product (93) sufficient to allow its structure to be assigned, it obviously was desirable to attempt to improve upon the yield of 15%. The reaction was repeated using Montmorillonite K10 clay as a solid acid catalyst. This reagent has been used previously in acid-catalyzed reactions such as poφhyrin syntheses, as it appears that the pore size of the clay is a good fit for allowing the entry of a poφhyrin, while being small enough to hold such a species in a restricted conformation suitable for intramolecular cyclization reactions. The addition of a small amount of activated Montmorillonite clay to the toluene solution of the imine (92) doubled the yield of (93) to 30 % after overnight reflux. However, although the solvent and reaction time were varied, no further improvements were made to the efficiency of the cyclization, since the competing hydrolysis reaction limited the yield by destroying the imine starting material. Significant quantities of the aminopoφhyrin (91 ) were recovered after each cyclization reaction, and this could be reused in the glyoxal condensation to regenerate (92). In order to see if the cyclization would have a different outcome if performed in the absence of air, in analogy with some puφurin syntheses, the reaction was performed under nitrogen. However, the same mixture of isomers was obtained as previously. In an attempt to convert the isomeric mixture to a single compound. 27 RE TIFIED S ULE 91 efforts were made to reduce the ethylidene double bond of (93) by catalytic hydro genation. but only decomposition products resulted. The mixture was then subjected to diimide reduction, a reaction known to reduce symmetric double bonds in preference to double bonds between heteroatoms, but in this case, suφrisingiy, reduction occurred at the carbonyl group, giving the isomeric mixture of alcohols (94) (the same mixture that initially had been expected to result from the cyclization reaction, see Figure 4). The identity of this compound was confirmed by reaction of the original isomer mixture with sodium borohydride, which gave the same species as its major product. The alcohol product (94) gave the following selected resonances in the 400 MHz Η NMR spectrum (only signals for the major isomer given, for clarity): a quartet at -0.23 ppm (methyl of the angular ethyl group), a doublet at 2.63 ppm (methyl of the ethylidene group), a singlet at 4.50 ppm (CHOH) a quartet at 6.62 ppm (methine of the ethylidene group), and a singlet at 7.88 ppm (CH=N). The longest- wavelength absoφtion peak was blue-shifted relative to (93) to 686 nm, reflecting the loss of the conjugated ketone. As attempts to convert (93) to a single compound were unsuccessful, the two isomers were separated by preparative thin layer chromatography. using a 0.5 mm silica plate. There was sufficient differentiation between the two bands to be able to acquire the major isomer (93a) in good purity. The minor isomer (93b). owing to slight trailing of the faster moving major compound, as well as to its presence in much smaller quantity, was more difficult to isolate in a completely pure state, but a reasonable Η NMR spectrum of it was obtained. NOE experiments on (93a) indicated that this compound has the Z-configuration. where the ethylidene methyl group is in the sterically less constrained position, pointing away from the angular ethyl group. This is analogous to the type B octaethylpuφurin situation, where the Z- geometry about the double bond was observed (although in that case no trace of the E-isomer was reported). The major isomer formed in the australochlorin synthesis also had the Z-geometry. The intention had been to produce a compound with an aromatic exocyclic ring. The major impediment to aromaticity in the cyclic product (93) is the presence 28 of the angular ethyl group. In steroid chemistry, an angular methyl group has be removed from androster-l,4-diene-3-one via a radical mechanism using zinc to generate the aromatic A ring (Figure 7A, see also K. Tsuda et al.. J. Org. Chem. .26, p.2614. 1961; and K. Tsuda et al. J. Org Chem.. 28, p. 795. 1963). As the exocyclic ring of (93) has a similar structure to the steroid A ring, it seemed possible this reaction might remove the angular ethyl group in an analogous way to yield the 3-hydroxypyridine structure (95) shown in Figure 7B. However, on refluxing the isomeric mixture (93) in pyridine in the presence of a large excess of zinc powder and a drop of water, only the zinc complex of the starting material (Zn- 93) was obtained. At this point it appeared that the possibilities of this compound had been exhausted, and other strategies towards the desired goal were considered. The replacement of glyoxal by glycolaldehyde in the condensation reaction would yield an imine (96) which might be more likely to lead to the desired product, as under acidic conditions the hydroxyl group would be lost and the resulting carbocation might easily lose a proton from the exocyclic ring to become aromatic (Figure 8). The analogous cyclization of the metal-free meso-(3- hydroxypropenyl)octaethylpoφhyrin has been reported to give quantitative yields of octaethylbenzochlorin. Unfortunately, despite several efforts, the condensation of glycolaldehyde with (91) was not achieved. Possibly this is a consequence of the difficulty of depolymerizing the dimeric glycolaldehyde to its monomer, although a reaction of this type with an aliphatic amine is reported to give good yields on stirring at room temperature in THF (see J.S. Davies et al., J. Chem. Soc, Perkin Trans., 2, p. 201, 1991). In a different strategy to form the desired imine alcohol, attempts were made to condense glyoxalic acid to form the imine carboxylic acid and then reduce this to the alcohol (96). It appeared that the condensation reaction was successful, but on work-up. despite being careful to maintain very slightly basic conditions, the product reverted to the starting material (91), suggesting auto- hydrolysis of the imine double bond by the carboxylic acid functionality. 29 R LE 91 The next approach was to use the amine (91) as a nucleophile. to attack alkyl halides such as 1.2-dibromoethane and l-bromo-2-(diethylacetal)ethane. and give the amines (98) and (99) (Figure 9). which could then be used in cyclization reactions. However, in both of these cases no reaction occurred: it seems that the nucleophilicity of the nitrogen atom is low, presumably as a consequence of delocalization of the lone pair into the aromatic system. It was clear from these unsuccessful reactions that a different method was necessary to form the desired acyclic precursors with nitrogen in the α-position. Efforts were then redirected to the β-pyridochlorins, with the expectation that they would in general be easier targets, and that the involved mechanisms would provide further insights into the efficient synthesis of α-pyridochlorins. Example 2 Svnthesis of β-pyridochlorin In considering methods for synthesizing the acyclic precursor to the β-pyridochlorin.. one might consider an approach very similar to that used for the α-pyridochlorin. and thus condensing the meso-formylpoφhyrin (56) (Figure 10) with an appropriate amine. to obtain the β-imine. Unfortunately, the necessary amine would be formamide. and amides do not possess the required nucleophilicity for the condensation. However, methylcarbamate. with greater nucleophilicity at the nitrogen atom, would produce a condensation product ( 100) potentially useful for the cyclization (Figure 10). This condensation was attempted but despite the use of a various conditions, no reaction was observed, and the mesoformylpoφhyrin was recovered unchanged. Another potential way to make compounds with nitrogen in the desired position would be to displace a leaving group on the meso-met yl group of the poφhyrin with a nitrogen nucleophile such as ammonia. The meso-hydroxymethyl compound (101) can be made by sodium borohydride reduction of the formyl group of (56). Efforts were made to convert this first to the p-toluenesulfonate ester, and then displace the p-toluenesulfonyl group with ammonia gas. During the first experiment, the intermediate ester was worked up, and an attempt was made to isolate 30 it. but it appeared to be very reactive, and decomposed. Consequently, a second reaction was run, and this time the in situ formed p-toluenesulfonate was reacted with ammonia gas. However in this case, only the hydroxymethyl starting mateπal was recovered. The next approach was a reductive amination of the meso- formyl compound (56), using ammonium acetate and sodium cvanoborohydnde. in another attempt to form the Once again, no reaction was observed. Having employed reactions well-known to work in typical organic systems, but without success, literature methods that described reactions specific to poφhynns were examined. There is one report of a condensation of an amine with meso- formyloctaethylpoφhyπn (56). the amine being β-alanine. and the yield was onlv 15 % (J-H Fuhrhop et al.. Ann Chem p 1537, 1976) As this synthesis had been attempted with methylcarbamate without success, it was not pursued further Another paper descπoes the condensation of m rø-formvletiopoφhyπn with aniline and p-anisidine. with very good vields. However, this approach requires the use of the amine as solvent, and the compounds thus produced, being aromatic Schiff s bases, are structurally further from the desired product than the alanine conjugate mentioned, and it was felt that the latter was a more realistic model for the present systems A more commonly used preparation of β-nitrogen-substituted poφhyπns involves the formation of the oxime of the OTeso-formylpoφhyπn using hydroxy lamine hydrochloride in refluxing pyridine (A W Johnson. J Chem Soc (C). p 794, 1966) The oxi e can be dehydrated to give the cyano group, which is reported to yield the /Heso-carboxy c acid by hydrolysis in sulfuπc acid, although this latter report has been shown to be in error (P S. Clezy et al.. Aust J Chem .27, p. 1 10, 1974) and will be discussed in more detail below The meso-cyanopoφhyπn (103) provides a route to the /neso-aminomethyl compound (104) via reduction (Figure 1 1) Hence /weso-cyanooctaethylpoφhynn was synthesized, and attempts were made to reduce it However, this compound was extremely resistant to the many reagents used (lithium aluminium hydπde, aluminium hydride, borane-dimethylsulfide complex, diborane, catalytic hydrogenation) In most cases, the starting mateπal was recovered unchanged, although in the case of catalytic 31 RECTIFIED SHEET (RULE 91) hydrogenation over palladium on charcoal, the poφhyπn nng was reduced at one of the meso-positions to give the green phloπn, which slowly reoxidized to the poφhyπn. Reduction of the nickel complex of the meso-cyano compound was also attempted, as the reactivity of a metallopoφhyπn can differ markedly from that of the corresponding free base. However, there was no improvement in reactivity; the only differences being a lack of reaction dunng catalytic hydrogenation, and the partial loss of the cyano-group dunng treatment with lithium aluminium hydnde. These disappointing results prompted the search for another strategy. As aforementioned, it was reported that the hydrolysis of meso- cyanoetiopoφhyπn in hot concentrated sulfuπc acid gave the carboxylic acid (A W Johnson. J Chem Soc (C), p. 794, 1966) Clezy et al. remvestigated this reaction and discovered that the product was in fact the amide. The same reaction was performed on the octaethylpoφhyπn analog with identical results, and despite using vanous forcing conditions, no further hydrolysis of the amide ( 105) was seen This amide is a potentially useful intermediate in the synthesis of cyc zation precursors (Figure 12), as it would lead via reduction to the desired ( 104) Unfortunately, reduction of the amide ( 105) using lithium aluminium hydπde in dry THF under nitrogen resulted in formation of the meso-cyano compound (103), l e. the dehydration product This was formed in 35 % yield, with the remainder of the vield being decomposition products, suggesting the possibility that the aminomethyl product was formed, but that it was too unstable to be isolated The nickel complex of the amide was also synthesized in order to see if it would be more easily reduced by treatment with the same reagent. In this case, no reaction occuned at room temperature, but after refluxing, the major product was nickel octaethylpoφhynn. This follows the same trend as with the nickel cyano-deπvative. the nickel complexes appear to readily lose their mesσ-substttuents under basic conditions. Thus, despite many efforts, no practical method for prepanng the desired aminomethyl-substituted poφhyπn ( 104) had been found. However, the meso-amide (105) could be produced. Although no progress had been made in reducing this compound, it was hoped that it could be converted into a cyclic chlorin with an 32 RECTIFIED SHEET (RULE 91) excocychc amide functionality, and that this cyclic product would prove more amenable to reduction (Figure 12). In order to make the precursor to the cyc zation step using the amide, it was necessary to alkylate the nitrogen atom with a functionalized methyl group susceptible to nucleophilic attack under acidic conditions, ideally a formyl or a hydroxymethyl group. Amides can be hydroxymethylated at the nitrogen atom using paraformaldehvde and base. A small-scale test reaction was run using sodium methoxide as base in refiuxmg THF: this lead to the formation of a more polar compound with a Η NMR spectrum consistent with the desired product (106). Subsequent tπals suggested that the quantity of base was crucial to the success of the reaction, as a large excess lead to the formation of a more polar compound which was initially attributed to the doubly alkylated species, produced by deprotonating the nitrogen atom of the N-hydroxymethyl compound and reacting with a second equivalent ot paraformaldehvde. As it evidently was important to control the quantity of base used, sodium methoxide was replaced by n-butylhthium. as this is easier to measure out in small quantities. Using 1.5 equivalents of this base in THF at room temperature, a 70% yield of the meso- N-hydτoxy methyl)amιnocarbonyloctaethylpoφhvπn (106) was obtained (Figure 13). The nickel complex of the N-hydroxymethyl compound was also of interest, as the cyc zation outcome can vary depending on whether the free base or metallated species is used (for example, refluxing the metal-free meso-acrylaldehyde of octaethylpoφhyrin in acetic acid leads to the puφuπn, whereas the nickel complex is unaffected by these conditions, see D.P. Arnold et al., JCS Perkin I, p. 1660. 1978). Therefore the metal-free form of (106) was subjected to the standard nickel complexing conditions: addition of nickel acetate tetrahydrate in refluxing DMF. Unfortunately, upon work-up of this reaction, it was found that the hydroxymethyl group had been lost, and the product was the nickel amide (Ni-105). The hydroxymethyl group was also lost under the conditions used for El (electron impact) mass spectrometry (at 200°C). so FAB (fast atom bombardment) was used for these compounds. The metallation with nickel was run at 50°C, and a 70 % yield of the desired product (Ni-106) was obtained after 3 days (the remainder of the recovered 33 R material was the unchanged starting matenal). In view of the very slow metallation at the lower temperature necessary to preserve the mtegπty of the compound, it seemed more efficient to make the nickel derivative by hydroxymethylation of the nickel amide (Ni-105). This was found to work well, although the first tnal using 1.5 equivalents of n-butylhthium resulted in a poor vield. or the desired compound and a large amount of more polar mateπal Again, it was initially believed that this side-product was the result of bis- hydroxymethylation at the nitrogen atom, but m this case there was now enough compound to study spectroscopically. and it was found to be the product of hydroxymethylation at the N-hydroxymethyl oxygen atom (Ni-107, Figure 14) This is an obvious result, given the relative pKa's of an alcohol (15 to 19) compared with an amide (25) Consequently, the reaction was repeated using 0 5 equivalents of n-butylhthium, to minimize formation of the side-product, and a 76 % yield of the target molecule was obtained It is interesting to note the differences between the reaction using the metal-free amide and that with the nickel amide, the latter appears to require only catalytic amounts of base, whereas the former requires at least stoicheometπc quantities. Presumably this is due to the central NH's in the metal-free compound, one of which is deprotonated by the base to give the monoa on- apparentlv the conditions are not such that the dianion is formed, as fewer than 2 equivalents of base are necessary Both the metal-free and the nickel-complexed N-hydroxymethylamides ( 106) and (Ni-106) had been prepared, ready for cyclization studies. The first experiment entailed stirring the nickel complex in dichloromethane solution with a drop of the Lewis acid, boron tπfluoπde etherate This represents one method for making metallated benzochlorins from their acyclic precursors On work-up after three hours, there appeared to be two major products (Nι-108, present in different proportions), both more polar than the starting mateπal. An attempt was made to separate these compounds by chromatography, but only the less polar one was obtained pure, as both compounds had poor solubility and streaked throughout the column A Η NMR spectrum of the pure compound was run, but very broad signals resulted, and this was thought to arise from aggregation of the compound in solution However, the visible 34 RECTIFIED HEET RULE 91 spectrum did give cause for optimism, as it displayed a large absoφtion peak at 646 nm, suggestive of a nickel chlorin. The next cyclization attempt involved treatment of the nickel complex (Ni- 106) with concentrated sulfuric acid. A peak was seen in the visible spectrum of the neutralized reaction mixture at 680 nm, indicative of a metal-free chlorin. On work- up a green-brown product ( 108) slightly more polar than the starting material was isolated in 70 % yield. Although by TLC this appeared to be one compound, the Η NMR spectrum showed it to be a 1:1 mixture of two isomers. The visible spectrum of the isolated mixture showed a double long-wavelength absoφtion peak at 680/686 nm. The Η NMR spectrum was reminiscent of that of the isomeric α-pyridochlorin mixture (93) made previously, and it was obvious that the two isomers arose from the same ethylidene functionality as in that case. The compound was assigned the structure shown below. The metal-free cyclization precursor ( 106) was treated with sulfuric acid, with identical results. The O-hydroxymethylated side-product (Ni-107) (Figure 14) was also subjected to the same conditions, resulting in isolation of the same product. Finally, the mixture of nickel lactams (Ni-108) from the boron trifluoride reaction was stirred with concentrated sulfuric acid. Again, the same 1:1 isomeric mixture of metal- free lactams (108) was obtained. It is interesting to note that although there appears to be a major and a minor isomer formed in the BF 3 reaction, on stirring with sulfuric acid, a 1:1 mixture results - obviously equilibration occurs under strong acid conditions. 35 RECTIFIED SHEET RULE 91 Once again, the products formed via loss of a proton from the β-ethyl group had been formed, rather than the desired compound that would result from a 1.2- dialkyl shift. A number of different cyclization conditions were investigated in the hope of finding a method that would lead to the target compound. The nickel N- hydroxymethylamide (Ni- 106) was refluxed in chloroform with the acidic clay Montomorillonite K10 (the successful catalyst in the earlier cyclization reaction to make the α-pyridochlorin-type structure). However, in this case, although a trace of cyclized material was detected, the major products were nickel octaethylpoφhyrin and the nickel amide, with only a small amount of starting material. It is suφrising to see that the meso-substituent was lost so easily under acidic conditions, as has already been observed under strongly basic conditions. Another cyclization was run using the metal-free N-hydroxymethylamide ( 106) in trifluoroacetic acid, and adding concentrated sulfuric acid dropwise until cyclization occuned. Here the thought was that one of the two isomers would be favoured, and the lower acid strength would prevent equilibration to the 1: 1 mixture thus far seen. Unfortunately in this case, no cyclization occuned until a large quantity of sulfuric acid was added, and the usual isomeric mixture (108) was obtained. The next line of approach was to synthesize the metal-free and nickel N- formylamide analogues (109) and (Ni-109). as there was an interest in determining if these would cyclize in the same manner, or if they might give the coπesponding cyclic imides (1 10) (Figure 15). The N-formylamides were produced by tetrapropylammonium perruthenate (TPAP) oxidation of the N-hydroxymethylamides (see W.P. Griffith, et al., J. Chem. Soc. Chem. Commun. p.1625, 1987). The oxidations proceeded smoothly to give the compounds in reasonable yields. The nickel complex (Ni-109) was treated with concentrated sulfuric acid, but no cyclization was observed, and the demetallated N-formylamide (109) and demetallated amide (105) were recovered. Dichloromethane/boron trifluoride etherate conditions were tried, but yielded only nickel octaethylpoφhyrin and a small amount of starting material. The metal-free N-formylamide (109) was then treated with dichloromethane/boron trifluoride etherate. but on work-up only starting material and the poφhyrin amide were obtained. Finally, cyclization of the metal-free compound 36 RECTIFIED SHEET (RULE 91) (109) in refluxing chloroform with Montmorillonite clay was attempted, and in this case the resulting compounds were starring material and octaethvlpoφhnrin. In conclusion, the acid treatment of the N-formylamides appears to lead preferentially to hydrolysis of the N-formyl moiety rather than to cyclization. Returning to the lactam (108), efforts were made to reduce the amide functionality which had resisted such a reaction before cyclization. However, once again, lithium aluminium hydride proved ineffective, both at room temperature and in refluxing TE. In each case, starting material was recovered. Attempts were also made to remedy the fact that ( 108) was present as a mixture of isomers. rather than as a single compound. Catalytic hydrogenation over palladium on charcoal was performed on the isomeric mixture. This reaction met with some success, although it proved difficult to obtain reproducible results. During some experiments, no reduction was seen, while in others, good results were achieved. This may be due to the presence of small amounts of impurities that poisoned the catalyst. One observation common to all experiments was the formation of a large quantity of a more polar pink compound which appeared from its visible spectrum to be non- poφhyrinic. possibly arising from reduction of the poφhyrin ring. Unfortunately, this compound did not reoxidize to the chlorin on stirring in air. or even after the addition of an oxidizing agent such as 1.2-dichloro-4.5-dicyanoquinone (DDQ). Hence the yields for this reaction, even when "successful", were prohibitive. However, a single poφhyrinic reduction product (1 1 1) was produced, reaction presumably taking place only on the least hindered side of the molecule, i.e. that opposite the angular ethyl group (it should be noted that both the α- and the β-pyridochlorins synthesized so far possess a chiral centre, and so exist as a mixture of optical isomers). The reduced compound exhibited a small blue-shift in the visible spectrum, the double peak at 680/686 nm becoming a single peak at 670 nm. reflecting the loss of the conjugated double bond of the ethylidene group. Since the nickel complex of the lactam (Ni-108) gave very broad signals in the 'H NMR spectrum, there was interest in seeing whether other metal complexes displayed similar behavior. Consequently the lactam free base (108) was metallated 37 RE RULE 91 with zinc. The zinc complex (Zn-108) displayed the same broadness in the Η NMR spectrum as previously seen with the nickel complex, and so it appears likely to result from strong association of the complexed metal from one molecule with the amide group of another molecule, leading to aggregation in solution. During the course of these efforts to produce a β-pyridochlorin, a report came to light detailing the synthesis of a we5O-isocyanopoφhyrin (112) (see P.S. Clezy, et al., Aust. J. Chem., 27, p. 1003. 1974). This was relevant to these studies as it provided an alternative approach to the formation of the α-pyridochlorin. The following section describes the strategy developed using this compound, and the subsequent results. Example 3 Additional approach to svnthesis of α-pyridochlorin via meso- isocyanooctaethylpoφhyrin The meJO-isocyanopoφhyrin (112) is produced by the dehydration of the conesponding meso-formamide (1 13), which in turn is formed by a Beckmann reanangement of the weso-acetoxime (1 14) in concentrated sulfuric acid (Figure 16). A Passerini reaction (I Ugi ed. Isonitriles, Academic Press, New York, 1971) of the isocyanide with formaldehyde and acetic acid as attempted in an effort to obtain the alkylated amide (1 15) shown in Figure 17. The product of this reaction would have been set up for acid-catalyzed cyclization to give a chlorin. However, on work- up, only the formamide (113) was isolated, formed by acid hydrolysis of the isocvanide. The next strategy involved reacting the isocyanide with formaldehyde in the presence of the Lewis acid, boron trifluoride etherate. It was envisaged that the nucleophilic isocyanide carbon atom would attack the carbocation of the formaldehyde-BF 3 adduct, to give the analog of the β-pyridochlorin cyclization precursor, with the nitrogen in the α-position (116), Figure 18. 38 RECTIFIE SHEET RULE 91 Formaldehyde gas was bubbled through a flask contaimng toluene, giving a solution of formaldehyde, to which was added boron trifluoride etherate. This solution was added to the solid isocyanide. and stiπed at room temperature. After work-up, two compounds were isolated: the major product was the formamide (1 13), formed by hydrolysis of the isocyanide by BF 3 . The other product was polar and highly insoluble. Spectroscopy showed this latter compound to be the desired species (1 16) Test reactions were run to see if (1 16) was susceptible to cyclization in acid, but neither reaction in dichloromethane with BF 3 catalysis, nor reaction in concentrated sulfuric acid lead to any trace of a cyclized product, and the starting material was recovered unchanged. In order to see if the nickel complex would be more prone to cyclization, the C-hydroxymethylamide was metallated with nickel, and this product (Ni-1 16) was subjected to the same reaction conditions descnbed for the metal-free compound The only reaction seen to occur was demetallation. Finally, attempts were made to oxidize the metal-free C-hydroxymethylamide ( 1 16) using TPAP to form the C-formylamide. in the hope that this might change the reactivity of the chain in favor of cychzation; however this oxidation reaction failed. The lack of reaction of the C-hydroxymethylamide (1 16) compared with the N-hydroxymethylamide (106) can be explained by the relative stabilities of the carbocations which must be formed in order for cyc zation to occur (see Figure 19). In the case of the N-hydroxymethylamide, the carbocation is adjacent to a nitrogen atom, and hence can be resonance-stabilized by the lone pair on that atom This stabilization allows the cation to exist long enough for the double bond to attack it, leading to cyc zation. In the case of the C-hydroxymethylamide. the carbocation would be created adjacent to a carbonyl group, i.e., adjacent to a partially positive carbon. Formation of the carbocation would lead to the highly unstable situation where two positive (or partially positive) atoms exist side by side. The lack of cyc zation suggests that such a species either does not form at all, or its lifetime is too short to allow nucleophilic attack on it, leading to cyclization. It was also intended to make more nickel C-hydroxymethylamide (Ni-1 16) by C-alkylating the nickel isocyanide (Ni-1 12) with paraformaldehyde/BF-, Instead of 39 RE L 91 obtaining the expected more polar hydroxymethyl compound, one major non-polar product was isolated, with an unusual visible spectrum (intense peaks at 406. 430, 496. and 680 nm and a low intensity peak at 814 nm). The Η NMR spectrum showed by the presence of a six hydrogen triplet at -0.05 ppm that this compound possesses a gem-diethyl group, which lead to the suggestion that a cyclization had occuned in situ to give the unsaturated lactam (Ni-117). However, this structure was not consistent with the Η NMR spectrum, as there were no signals conesponding to the proton α to the NH, even if the NH signal itself were too broad to be distinguished. Despite its low polarity, the product was very slow to dissolve, and had a melting point higher than most octaethylpoφhyrin derivatives. These facts, coupled with the atypical visible spectrum, suggested that the compound might be a dimer. and so it was submitted for FAB mass spectrometry, which confirmed this. A mechanism can be drawn for the formation of a dinner (Ni- 1 19) consisting of two units of (Ni-1 18) joined at the carbon α to the nitrogen atom (Figure 20). 40 T RULE 91 This mechanism requires the addition of two equivalents of IT " and two electrons per molecule of dimer. Excess BF 3 was used, and accounts for the acid requirement, but reduction under these conditions might seem harder to explain. However, the formation of nickel octaethylbenzochlorin (Ni-17, see also Figure 1 ) also requires a formal reduction to take place under similar conditions, and it is suggested that nickel poφhyrin complexes can act as electron donors under acidic conditions (see D.P. Arnold et ai.. JCS Perkin. I, p. 1660, 1978). It should be noted that the best yields of this reaction were approximately 50%, and a large quantity of non-poφhyrinic decomposition product was always obtained, reinforcing the possibility of electron donation from two poφhyrin molecules to two others to form the dimer. followed by decomposition of the two poφhyrin cation radicals. Also, the fact that the reaction only occurs when the nickel complex is used, and no cyclizaton/dimerization is seen with the metal-free compound, suggests that the nickel is playing an important role. The proposed structure would be consistent with the H NMR spectrum, the only signals not observed being the NH protons. The final structure (Ni-120, Figure 20) was ascertained by X-ray crystallography, and was significantly different to that proposed. This structure contains a pyrido[3,2-b]-pyridine unit linking the two chlorin molecules. It possesses two fewer hydrogen atoms than the proposed structure, and fits better with the mass spectrum (however, without the hindsight made possible by a crystal structure, it would have been difficult to be certain of the exact number of hydrogen atoms in the dimer simply by FAB mass spectrometry, as such an ionization method sometimes 41 RECTIFIED SHEET RULE 91 gives rise to molecular ion peaks coπesponding to the gain of one or more hydrogens). A mechanism can be drawn for the formation of this dimer which is catalytic in acid, and has no need for the reduction step (Figure 21). Finally, this structure is completely consistent with the Η NMR spectrum, and there are no "missing" signals. This result was extremely interesting. The objective of this Example was to create a chlorin with a fused pyridine ring, which so far was difficult to effect. In this reaction, finally this objective had been achieved, but "in duplicate." in the form of a dimer. In all previous cyclizations. the alkyl 1 ,2-shift did not occur, and instead the ethylidene product resulting from the loss of a proton from the ethyl group was obtained. This did not appear to happen in this case. However, when the dimerization reaction was run on a larger scale, and the product was very carefully isolated, another compound was seen running just behind the dimer (Ni-120) on TLC. After purification by column chromatography, Η NMR and mass spectra of this fraction were obtained, and it was revealed to be the ethylidene analogue of the dimer, in which there is one ethylidene group, one angular ethyl group, and one gew-diethyl group (Ni-121). As there is now no symmetry in the molecule, the Η NMR spectrum is much more complex. Two noteworthy points about this minor product are (a) that it exists as one isomer, i.e.. there is only one geometry about the ethylidene bond (although the presence of a chiral cenetre at the angular ethyl group gives rise to optical isomers) and (b) that its visible spectrum differs greatly from that of the major dimeric product, possessing a Soret band at 410 nm and very little absoφtion at longer wavelengths. The angular hydrogen atom at the position of fusion of the two pyridine rings prevents these rings from becoming aromatic, and this presumably prevents interaction between the two poφhyrin moieties and accounts for the more poφhyrin-like visible spectrum. 42 RECTIFIED SHEET RULE 91 The major nickel dimer (Nι-120) was demetallated in concentrated sulfuric acid to give a very insoluble grey product ( 120) The ' H NMR spectrum of this product showed two new singlets at 4 97 ppm 5 82 ppm. exchangeable with D 2 0, but no signals were seen upfield of 0 ppm. in the region where the central NH protons are usually observed It is possible that the pyπdine nitrogens are more basic than the central nitrogens, and that it is the protonated pyridine NH's that give rise to the new signals High resolution FAB mass spectrometry confirmed that demetallation had occuπed Further confirmation was obtained by complexing the demetallated compound with zinc, the zinc dimer (Zn-120) thus obtained displays a H NMR spectrum very similar to that ot the nickel complex The nickel, zinc and metal-free compounds all possess similar visible spectra Example 4 α-Pyridochlonn svnthesis via cvchzation of the metallated formylmethylimine The differences in reactivity to acid of the nickel-complexed and metal-free prompted the review of all previous attempts at synthesizing pyridochlorins. in order to be certain every logical cyc zation method had been tried on both the metallated and metal-free substrates The first cyc zation that had been attempted, which gave rise to the immoketone (93), rather than the desired α-pyridochlonn. had been performed only on the metal-free precursor (92) For the sake of completeness, further investigations using the metallated compound (M-92) were conducted 43 RECTIFIED SHEET RULE 91) Metallation of the formylmethylimine (92, see Figure 3) was attempted under mild conditions, by stirring with zinc acetate at room temperature. However, this resulted in cleavage of the imine, to give the zinc amine (Zn-91). In light of this result, it was necessary to go back one step, to synthesize the zinc formylmethylimine by condensation of the zinc aminopoφhyrin with glyoxal. The condensation occurred in better yield than that with the metal-free amine: after refluxing overnight approximately 80% of the desired product (Zn-92) was obtained. Test experiments were run using various acids to effect the cyclization of the zinc formylmethylimine, as initially it appeared to be more acid-stable than the metal-free analogue. Unfortunately, acetic acid, trifluoroacetic acid and boron trifluoride etherate all gave rise to the metal-free amine (91). and no evidence of cyclization was observed. Hence the standard cyclization conditions of Montmorillonite K10 acidic clay and refluxing toluene were employed. After 24 hours reflux mostly starting material remained, according to TLC. but there was a small amount of more polar green product, as well as some zinc iminoketone (Zn-93) (this cospotted with an authentic sample produced by metaliating the iminoketone (93) with zinc). Refluxing was continued for a further 24 hours, then the mixture was worked up and purified by chromatography. The polar green material (Zn-123) was isolated in 10% yield, and 80% of the starting material was recovered. The visible spectrum of the new compound indicated it was a chlorin, with a prominent absoφtion peak at 678 nm. In contrast to the metal-free cyclic product (93), the Η NMR spectrum of this product showed no ethylidene peaks, but a six hydrogen triplet at 0.33 ppm suggested that the cyclization had occurred as originally anticipated, to give an aromatic exocyclic pyridine ring and a gem-diethyl group at the adjacent β-position. There were four peaks in the aromatic region, evidence of three /weso-hydrogens and one hydrogen on the exocyclic ring. This, coupled with the mass spectral data, showed that unlike in the benzochlorin synthesis, the hydroxyl group is retained on the ring. The exchangeable hydroxyl proton was not observed in the NMR spectrum, which had to be run in pyridine. as the compound was poorly soluble in other solvents. The new chlorin (Zn-123) was assigned the structure shown. 44 91 (Zn-123) This compound was difficult to obtain in a completely pure state, as it streaked badly during chromatography. It was demetallated in dichloromethane/trifluoracetic acid to give the metal-free species (123) in approximately 30% yield. It seems likely that this poor yield is a reflection of the impurity of the starting material rather than a measure of the inefficiency of the demetallation. The metal-free compound (123) was easier to purify, as it is less polar and runs in a much naπower band on the silica column. It possesses a visible spectrum with a strong absoφtion at 672 nm; the diagnostic peaks in the Η NMR spectrum are a six hydrogen triplet at 0.40 ppm and four aromatic peaks above 8 ppm, as well as a broad singlet at 3.06 ppm coπesponding to the OH proton. The synthesis of one of the target compounds had finally been achieved, using the very first method devised, but with one obviously crucial modification: using the metallated substrate for the cyclization rather than the free base. The (3- hydroxypyrido) chlorin (123) appears to be fairly stable to acid (in that it can be demetallated by treatment with acid without destruction of the chromophore), has good spectroscopic properties, and possesses sites for possible further derivation in its hydroxyl group and basic N-atom. Unfortunately, so far efforts to improve the yield have failed - approximately 5 mg product is obtained from 100 mg zinc formylmethylimine (Zn-92). However, the unused starting material is recovered unchanged, and can be recycled through the cyclization reaction many times. 45 RECTIF RULE 91 Additional information concerning the synthesis of such compounds is as follows. Octaethylbenzochlorin is synthesized via intramolecular cyclization of the metallated meso-acrylaldehyde substituted poφhyrin under acidic conditions. It seemed logical to follow a similar route to prepare octaethylpyridochlorin. Meso- aminooctaethylpoφhyrin 1 was condensed with glyoxal in THF/ethanol. After 24 hours refluxing the formylmethylimine product 2 was obtained in 69% yield. Intramolecular cyclization of 2 was achieved by overnight reflux in toluene in the presence of Montmorillonite KIO acidic clay. The two geometric isomers 3a and 3b were isolated in 31% total yield, in addition to a substantial quantity of the hydrolysis product, 1. The cyclic product consisted of a 4: 1 mixture of the two isomers, which were separable by preparative chromatography. The major component was examined by NOE spectroscopy, which revealed it to be the Z-isomer 3a. The isomeric mixture was treated with acid, in an attempt to bring about a 1.2-dialkyl shift and hence form the desired pyndochlorin 4. However, this reaction was unsuccessful, leading to decomposition of the chromophore. Experiments intended to hydrogenate the ethylidene double bond of the isomeric mixture to give a single isomer also failed. O Scheme 1. Synthesis of the isomeric chlorins 3a and 3b The presence or absence of a centrally-complexed metal can have a marked effect on the outcome of intramolecular cyclization reactions of poφhyrins. Therefore the cyclization of the zinc complex of 2 was studied. Zn-2 was prepared by condensation of the zinc aminopoφhyrin Zn-1 with glyoxal (metallation of 2 with zinc acetate led to hydrolysis of the imine. resulting in isolation of Zn-1). The cyclization was performed using the conditions described above (toluene. Montmorillonite KIO, heat), 46 RECTIFIED SHEET (RULE 91) and did indeed proceed differently to that of the free base 2. In this case, reaction was much slower, little change being discernable until refluxing had continued for 3 or 4 days. At this point only a trace amount of the expected product (the zinc complex of 3a/3b) was seen on TLC. A more polar green compound appeared to be the major product of the reaction (in addition to unreacted starting material. Zn-2, present as approximately 80% of the mixture). After work-up this compound was isolated in 20% yield and NMR analysis showed it to be the zinc complex of 4. Demetallation with trifluoroacetic acid gave the free base 4. Zn Scheme 2. Synthesis of pyridochlorin 4 Pyridochlorin 4 has a slightly red-shifted long-wavelength absoφtion (λmax = 672 nm), compared to octaethylbenzochlorin (λ max = 658 nm), and it possesses additional characteristics that might improve its performance as a photosensitizer over the latter. It is known that amphiphilic macrocycles, i.e., those bearing both hydrophobic and hydrophilic moieties display better tumour-localizing properties than photosensitizers without these properties. The presence of the polar hydroxypyridyl ring attached to the low polarity poφhyrin skeleton increases the amphiphilicity of 4. Octaethylbenzochlorin suffers from a lack of functional groups available for further derivatization; the preparation of analogues based on this compound requires additional synthetic steps in order to introduce such a functionality. In contrast, chlorin 4 possesses "built-in " sites at the hydroxypyridine ring that allow for more direct analogue formation. 47 RECTIFIED SHEET RULE 91) It will also be appreciated by those familiar with the art that the pyridinyl hydroxyl group such as shown for compound 4 directly above can be replaced by hydrogen under appropriate conditions, thereby providing an additional pathway to compounds containing a simple (unsubstituted) pyridine ring as exocyclic component. 3a: Η NMR (CDC1 3 ) δ: -1.78 (br s, IH. NH), -0.74 (br s. IH, NH), 0.50 (t, J=7.3 Hz, 3H, CH 3 ), 1.65-1.90 (m, 18H. 6CH 3 ), 2.81 (d. J=7.6 Hz. 3H, CH 3 =CH), 3.70-4.00 (m, 12H, 6CH 2 ), 4.09-4.26 (m, 2H, CH 2 ), 7.45 (q, J=7.5 Hz. IH, CH=CH 3 ), 8.15 (s, IH, CH=N), 9.31 , 9.51 , 9.57 (3s, 3H. me o-Ws); UV-Vis in CHCH 3 , λ nm (ε): 360 (4.33), 410 (4.82), 446 (4.67), 584 (3.66), 672 (3.79), 722 (4.03); Analysis calc'd for C, 75.34; H, 7.82; N, 1 1.56: found C, 74.89; H, 7.69; N, 1 1.15. 3b: Η NMR (CDCI 3 ) δ: -1.14 (br s. IH. NH). -0.74 (br s. IH. NH). 0.10 (t, J=7.3 Hz. 3H. CH 3 ), 1.68-1.84 (m. 18H. 6CH 3 ), 2.62 (d. J=7.5 Hz. 3H, CH 3 =CH), 3.70-3.95 (m, 12H. 6CH 2 ), 4.04-4.25 (m. 2H. CH 2 ), 7.72 (q, J=7.5 Hz. I H. CH=CH 3 ), 7.98 (s, I H, CH=N). 9.16, 9.43, 9.53 (3s. 3H. mrø-H's). 4: Η NMR (pyridine-d,) δ: 0.40 (t, J=7.3 Hz. 6H, 2CH 3 ), 1.63-1.81 (m, 15H, 5CH 3 ), 1.88 (t, J=7.3 Hz, 3H, CH 3 ), 2.78-2.91 (m, 2H. CH 2 ), 3.49-3.71 (m, 10H. 5CH 2 ), 3.85 (q, J=7.5 Hz. 2H. CH 2 ), 4.43 (q, J=7.3 Hz, 2H. CH 2 ), 8.40, 8.99, 9.58. 9.69 (4s, 4H, 3meso-H , s and CH=N) 13.06 (br s. I H, OH); UV-Vis in CHCH 3 , λ nm (ε): 412 (4.89), 490 (3.51), 522 (3.73), 556 (3.75), 616 (3.86). 672 (4.36); Analysis calc'd for C 38 H 47 N 5 O-0.5H 2 O: C, 76.22; H. 8.08; N, 1 1.70: found C, 75.96; H, 7.64; N, 1 1.67. Example 5 Preparation and Characterization of Compounds Presented below are details on the synthesis and characterization of compounds used in the practice of the invention. In summary, these Examples have described efforts to synthesize a number of chlorins with fused pyridine rings, analogous to the benzochlorins. Varying yields were achieved, with one of the prefeπed target molecules being prepared, while three other new types of cyclic chlorin were created. 48 RE TIFIED SHEET RULE 91 The first new chlorin that was prepared. (93), possesses an exocyclic nng containing a β-carbonyhmine functionality O=CCH=N. and an ethylidene group on the reduced pyπole ring Its longest-wavelength absoφtion in the visible spectrum is at 722 nm (ε = 10800) Both possible geometnc isomers were produced, in a ratio of approximately 4 1. and these two isomers could be separated by chromatography. although they were not amenable to catalytic hydrogenation to give a single isomer The carbonyl group could be reduced with sodium borohydπde to give the coπesponding alcohol. Attempts to aromatize ring were unsuccessful. This compound could be metallated with nickel or zinc under standard conditions The second type of chlonn synthesized, (108). contains an exocyclic lactam, with an absoφtion at 686 nm ( t = 34800) Again, an ethvhdene group is attached to the reduced pyπole ring, but in this case the ratio ot geometric isomers is 1 1. and these proved substantially inseparable bv chromatography The metal complexes of this compound appear to exist in solution as aggregates, indicating the high affinity of the centrally complexed metal for the lactam amide functionality of another molecule Reduction of the amide group failed, but the ethylidene group underwent catalytic hydrogenation to give a single diastereomer Metallation and demetallation reactions were successful The third class of compounds formed. ( 120). consists of a dimer of two chlorin molecules possessing exocyclic pyridine rings, fused to each other through the pyndine rings. These compounds display very unusual visible spectra, for example, the nickel complex possesses an absoφtion peak at 814 nm (ε = 13600) The X-ray crystal structure shows that the molecule is essentially planar Metallation and demetallation reaction were successful The fourth and final class of compounds synthesized, (123), contains an exocyclic 3-hydroxypyrιdιne ring, with the pyridine N-atom positioned α to the poφhyrin me5ø-posιtιon The metal-free species has a \ lsible spectrum with its longest-wavelength absoφtion at 672 nm ( ε = 22700) 49 Preparation and characterization of compounds (92) 5 -Formylmethyliminooctaethylpoφhyrin 5-Aminooctaethylpoφhyrin (91) 194 (100 mg, 0.18 mmol) was dissolved in dry THF (10 mL). Glyoxal trimer dihydrate (250 mg, 1.2 mmol) was dissolved in ethanol (10 mL) with heating. The latter solution was added to the former, and the mixture was refluxed overnight. The solvent was removed in vacuo and the residue purified by chromatography (flash silica, dichloromethane eluent. polarity of eluent increased to 5 % ethyl acetate in dichloromethane after the product had been eluted to elute starting material), giving the product (74 mg. 69 %) and unreacted starting material (24 mg). 1 H NMR (200 MHz. CDC1 3 ) δ 1.80 (t, J=7 Hz. 6H, 2 x CH 3 ), 1.88-2.12 (m, 18H, 6 x CH 3 ), 3.92-4.26 (m, 16H, 8 x CH 2 ), 7.84 (d, J=9 Hz. IH. CHCHO), 9.95 (s. IH, 1 meso-H), 10.11 (s, 2H. 2 meso-H's), 10.45 (d, J=9 Hz, IH. CHO); 13 C NMR (50 MHz, CDC1 3 ) δ 16.21. 18.45, 18.51, 19.77. 22.15. 96.69, 98.19. 102.62. 128.26, 137.18. 140.30, 141.53, 142.36. 143.67. 143.89. 145.73. 169.16. 191.46; UV-Vis (CHC1 3 (log ε)) λ max 396 (4.96), 458 (4.67), 502 (3.98), 582 (3.91), 660 (3.64) nm; MS (El) mJe 589 (M + , 100 %), 560 (M + -29. 32 %). (93) Cyclic iminoketone (diastereomeric mixture) The formylmethylimine (92) (61 mg, 0.10 mmol) was dissolved in toluene (10 50 RECTIFIED HEET RULE 91 mL). Montmorillonite KIO acidic clay (50 mgj was activated by heating with a heat- gun in a test tube for 5 min. until all water had been driven off. and was then added to the solution. The mixture was refluxed overnight, then filtered and the solvent was removed in vacuo. The residue was purified by chromatography (silica, dichloromethane eluent) to give the cyclized product (19 mg, 31 %) as 2 diastereomers and unreacted starting material (14 mg). The 2 isomers were separated by prep. TLC on a 0.5 mm thick silica plate, eluting with dichloromethane:hexanes 1 :1. UV-Vis (CHC1 3 (log ε)) λ max 360 (4.33), 410 (4.82), 446 (4.67), 584 (3.66), 672 (3.79), 722 (4.03) nm; MS (El) m/e calc'd for C 38 H 45 N 5 O: 587.36243. found 587.36313; 587 (M + , 40 %), 558 (M + -29. 100 %); Singlet Oxygen Test: Negative. (93a) Cyclic iminoketone (major isomer) Present as approximately 80 % of the mixture. m.p. 214-215°C; R F 0.36 (silica - CH 2 Cl 2 :hexanes 1 :1); Η NMR (400 MHz, CDCl 3 ) δ -1.18 (br s, IH, 1 x NH), -0.74 (br s. IH, 1 x NH), 0.50 (t, J=7.3 Hz. 3H, CH 3 of angular ethyl group). 1.65-1.90 (m. 18H, 6 x CH 3 ), 2.81 (d. J=7.6 Hz. 3H. CH 3 CH=), 3.70-4.00 (m. 12H. 6 x CH 2 ), 4.09-4.26 (m. 2H, CH 2 of angular ethyl group), 7.45 (q, J=7.5 Hz. 1 H. CHCH 3 ), 8.15 (s. 1 H, CH=N), 9.31 (s, 1 H, 1 meso-H), 9.51 (s, IH, 1 meso-H), 9.57 (s, IH, 1 meso-H); Analysis calc'd for C 38 H45N5O-H 2 O: C, 75.34; H, 7.82; N, 1 1.56; found: C, 74.89; H, 7.69; N, 1 1.15. (93b) Cyclic iminoketone (minor isomer) Present as approximately 20 % of the mixture. R F 0.27 (silica - CH 2 Cl 2 :hexanes 1 : 1 ); ' H NMR (400 MHz. CDC1 3 ) δ - 1.14 (br s. IH, 1 x NH), -0.74 (br s. IH. 1 x NH), 0.10 (t, J=7.3 Hz, 3H, CH 3 of angular ethyl group), 1.68-1.84 (m, 18H. 6 x CH 3 ), 2.62 (d. J=7.5 Hz, 3H, CH =CH), 3.70- 3.95 (m, 12H. 6 x CH 2 ), 4.05-4.25 (m. 2H. CH 2 of angular ethyl group). 7.72 (q, J=7.5 Hz, IH. CH 3 =CH). 7.98 (s. IH. CH=N). 9.16 (s. IH, 1 meso-H), 9.43 (s. IH. 1 51 RULE 91 meso-H). 9.53 (s, IH, 1 meso-H). (Zn-93) Cyclic iminoketone-zinc (II) (diastereomeric mixture) The cyclic product (93) (6 mg, 0.010 mmol) was dissolved in dichloromethane (1 mL). and zinc acetate dihydrate (10 mg, 0.046 mmol) in methanol (0.5 mL) was added. The mixture was refluxed 2 hours, then the solvent was removed in vacuo and the residue purified by chromatography (silica, dichloromethane eluent) to give the metallated product (4 mg, 62 % yield). R F 0.64 (silica - CH 2 C1 2 ); 'H NMR (200 MHz. CDC1 3 ) (NB: only peaks for major isomer iisted) δ 0.50 (t. J=7.5 Hz, 3H, CH 3 of angular ethyl group), 1.51-1.75 (m, 18H. 6 x CH 3 ), 2.70 (d, J=7.5 Hz. 3H. CH 3 CH=). 3.49-3.75 (m. 12H. 6 x CH 2 ), 3.80-3.97 (m. 2H. CH 2 of angular ethyl group), 7.35 (q, J=7.5 Hz. IH, CHCH 3 ), 7.95 (s. 1H. CH=N). 8.85 (s. I H. 1 meso-H). 9.05 (s, I H. 1 meso-H), 9.13 (s, I H, 1 meso- H); UV-Vis (CH 2 C1 2 ) λ max 412, 454. 688 nm. (94) Cyclic iminoalcohol (diastereomeric mixture) The cyclic product (93) (8 mg, 0.013 mmol) was dissolved in dichloromethane (5 mL). Sodium borohydride (10 mg, 0.26 mmol) in ethanol (0.5 mL) was added, and the mixture was stiπed at room temperature for 1 hour. The mixture was then poured into water and extracted with dichloromethane. After work-up, the residue was purified by prep. TLC (0.2 mm thickness silica plate, dichloromethane eluent) to give the product, present as a mixture of diastereomers (2 mg, 25 % yield). •H NMR (400 MHz, CDC1 3 ) (NB: only peaks for major isomer listed) δ -2.25 (br s, IH, 1 x NH), -1.69 (br s. IH. 1 x NH), -0.23 (t. J=7.4 Hz. 3H. CH 3 of angular 52 RECTIFIED SHEET RULE 91 ethyl group), 1.50-2.00 (m, 18H, 6 x CH 3 ), 2.63 (d, J=7.5 Hz. 3H. CH 3 CH=), 3.70- 4.40 (m, 14H. 7 x CH 2 ), 4.50 (s. IH. CHOH). 6.62 (q. J=7.4 Hz. I H, CHCH ), 7.88 (s. IH. CH=N), 9.42 (s, IH. 1 meso-H), 9.64 (s. IH. 1 meso-H). 9.71 (s, IH, 1 meso- H); MS (El) m/e 589 (M + , 50 %). 587 (M + -2. 45 %), 560 (M + -29, 75 %), 558 (M + -31, 100 %); Singlet Oxygen Test: Positive. (105) 5-Aminocarbonyloctaethylpoφhyrin Prepared according to the method of Clezy et al. 207 m.p. 296-299°C (liι.295-297); R F 0.32 (silica - CH 2 C1 2 / 5 % AcOEt); UV-Vis (CH C1 2 ) λ max 400. 502, 536. 572. 624 nm. (106) 5-((N-Hydroxymethyl)amιnocarbonyl)octaethylpoφhyrin The amide (105) (28 mg. 0.048 mmol) was dissolved in dry THF (10 mL) under nitrogen. 1.6 M n-BuLi in hexanes (30 μL. 0.048 mmol) was added, and the mixture was stiπed under nitrogen for 5 min. Paraformaldehvde (3 mg) was added. and the mixture was stiπed at room temperature for 1 hour. Acetic acid (2 drops) was added, then the solvent was removed in vacuo and the residue was purified by chromatography (flash silica, eluent 5 % ethyl acetate in dichloromethane), to give unreacted starting material (6 mg) and the product ( 15 mg, 51 % yield). m.p. >300°C; R F 0.16 (silica - CH 2 C1 2 / 5 % AcOEt); 'H NMR (200 MHz, CDC1 3 ) δ -3.50 (v br s, 2H. 2 x NH), 1.33 (t, J=7.5 Hz. 6H, 2 x CH 3 ), 1.78-1.94 (m, 18H, 6 x CH 3 ), 3.44-3.62 (m, 4H, 2 x CH 2 ), 3.84-4.12 (m, 12H, 6 x CH 2 ), 4.42 (d, J=6 Hz. 2H, CH 2 OH), 6.50 (t. J=6 Hz, I H. NH), 9.88 (s. IH, 1 meso-H), 10.10 (s, 2H, 2 meso-H's); 1 C NMR (50 MHz. CDC1 3 ) δ 17.55. 18.47. 19.57, 20.30. 65.18. 96.33. 53 L 91 97.02. 102.83, 110.80. 140.73, 141.48. 142.09. 142.25, 143.13. 144.58. 145.32, 172.10; UV-Vis (CH 2 C1 ) λ max 400. 502. 536. 570. 624 nm; MS (FAB (thioglycerol matrix)) m/e 608 (M + , 100 %). (Ni-105) (5-Amιnocarbonyloctaethylpoφhyrinato)nickel(II) The amide (105) (31 mg. 0.054 mmol) was dissolved in dimethylformamide (5 mL) and nickel acetate tetrahydrate (30 mg, 0.12 mmol) was added. The mixture was refluxed overnight, then poured into water, extracted with ethyl acetate, the extracts dried over sodium sulfate. and the solvent removed in vacuo. The residue was purified by flash chromatography (silica, eluent 5 % ethyl acetate in dichloromethane) to give the metallated product (28 mg. 82 % yield). m.p. 272-274°C; R F 0.44 (silica - CH C1 2 / 5 % AcOEt); l H NMR (200 MHz. CDCl 3 ) δ 1.60 (t. J=7.5 Hz. 6H. 2 x CH 3 ). 1.77 (t. J=7.5 Hz, 18H. 6 x CH 3 ), 3.73-3.99 (m, 16H, 8 x CH 2 ), 5.75 (br d. J=2 Hz, IH. 1 x NH), 6.38 (br d. J=2 Hz. IH. 1 x NH), 9.52 (s, IH, 1 meso-H), 9.60 (s, 2H. 2 meso-H' s); 13 C NMR (50 MHz. CDC1 3 ) δ 18.02, 18.17. 19.58, 21.21, 96.36. 96.86. 139.24. 140.10. 140.81, 143.25. 143.47. 143.69, 145.61. 172.50; UV-Vis (CH 2 C1 2 ) λ max 400. 524. 558 nm; MS (FAB (thioglycerol + CHC1 3 matrix)) m/e 634 (M + , 100 %). (Ni-106) [5-((N-Hydroxymethyl)aminocarbonyl)octaethylpoφhyrinato]nickel(II) The nickel amide (Ni-105) (30 mg, 0.047 mmol) was dissolved in dry THF (20 mL) and 1.6 M n-BuLi in hexanes (15 μL. 0.024 mmol) was added. This solution was stiπed for 5 min under nitrogen, then paraformaldehvde (4 mg) was added. The mixture was stiπed at room temperature for 1 hour, then acetic acid (2 drops) was added and the solvent evaporated. The residue was purified by chromatography (silica, eluent 5 % ethyl acetate in dichloromethane) to give the pink product (24 mg, 76 % yield). R F 0.25 (silica - CH 2 C1 2 ' 5 % AcOEt): 'H NMR (200 MHz. CDC1 3 ) δ 1.50 (t, J=7.5 Hz. 6H. 2 x CH 3 ), 1.68-1.85 (m. 18H. 6 x CH 3 ), 3.61-3.92 (m. 16H. 8 x CH 2 ), 54 5.08 (d. J=6.0 Hz, 2H, CH 2 OH), 6.58 (t, J=6.0 Hz. IH. NH), 9.48 (s. IH. 1 meso-H), 9.53 (s, 2H, 2 meso-H's); 13 C NMR (50 MHz, CDC1 3 ) δ 17.74, 18.09. 18.17, 19.54, 20.84. 65.67, 96.47, 96.88, 108.99. 135.29, 140.10, 140.77, 143.22, 143.39, 143.46, 145.51. 171.77; UV-Vis (CH 2 C1 2 ) λ max 398. 522. 558 nm; MS (FAB (thioglycerol + CHC1 3 matrix)) m/e 663 (M + , 30 %). (109) 5-(N-Formylaminocarbonyl)octaethylpoφhyrin The N-hydroxymethylamide (106) (5 mg, 0.008 mmol) was dissolved in dichloromethane (2 mL) to which anhydrous magnesium sulfate ( 10 mg) had been added. To this mixture was added N-methylmoφholine-N-oxide (1 mg), and the solution was stiπed for 5 min. Then tetrapropylammonium perruthenate (TPAP) (0.5 mg) was added and the mixture was stiπed for 30 min. The solvent was evaporated, and the residue purified by chromatography (silica, eluent dichloromethane) to give the pink product (4 mg. 80 % yield). R F 0.29 (silica - CH 2 C1 2 ); UV-Vis (CH 2 C1 2 ) λ max 402. 504, 538. 572, 624 nm; MS (FAB (thioglycerol matrix)) m/e 606 (M + , 100 %). (Ni-109) [5-(N-Formylaminocarbonyl)octaethylpoφhyrinato]nickel(II) The nickel N-hydroxymethylamide (Ni-106) (1 1 mg, 0.017 mmol) was dissolved in dichloromethane (2 mL) to which anhydrous magnesium sulfate (20 mg) was added. To this solution was added N-methylmoφholine-N-oxide (2 mg). The mixture was stiπed for 5 min, then tetrapropylammonium perruthenate (TPAP) (0.5 mg) was added. The mixture was stiπed for 30 min, then the solvent was evaporated, and the residue purified by chromatography (silica, dichloromethane eluent) to give 55 the pink product (7 mg, 64 % yield). R F 0.43 (silica - CH 2 C1 2 ); UV-Vis (CH 2 C1 2 ) λ max 398. 524. 560 nm; MS (FAB (thioglycerol + CHC1 3 )) m/e 662 (M + +l, 85 %). (Ni-107) [5-((N- Hydroxymethylmethylether)aminocarbonyl)octaethylpoφhyrinato]nickel-(II) Formed as a side-product in the synthesis of (Ni-106). especially when excesses of butyllithium were used. Η NMR (200 MHz. CDC1 3 ) δ 1.58 (t, J=7.5 Hz, 6H. 2 x CH 3 ), 1.70-1.88 (m, 18H, 6 x CH 3 ), 3.62-3.99 (m, 19H, 8 x CH + OCH 2 0 1- NH), 5.46 (d, J=8 Hz, 2H, NHCH 2 Q), 9.58 (s, IH, 1 meso-H), 9.64 (s, 2H. 2 meso-H's); MS (FAB (thioglycerol + CHCI 3 matrix)) m/e 693 (M\ 16 %). (108) Unsaturated lactam (mixture of isomers) The nickel N-hydroxymethylamide (Ni-106) (10 mg, 0.015 mmol) was dissolved in concentrated sulfuric acid (1 mL), and stiπed at room temperature for 1.5 hours. The solution was then poured into water, extracted 4 times with dichloromethane. washed successively with potassium carbonate solution and water, and the solvent evaporated. The residue was purified by chromatography (silica, eluent 20 % ethyl acetate in dichloromethane) to give the green-brown product as a 56 1 : 1 mixture of isomers (7 mg, 79 % yield). m.p. 293-294°C; R F 0.44 (silica - CH 2 C1 2 / 20 % AcOEt); >H NMR (400 MHz. CDC1 3 ) δ (selected peaks). -1.20 (br s. 2H, 2 x NH of one isomer), -0.62 (br s, 2H. 2 x NH of one isomer). 0.33 (t, 3H. Me of angular Et group of one isomer). 0.58 (t, 3H. Me of angular Et group of one isomer), 2.37 (d. 3H. CH 3 =CH of one isomer), 2.60 (d, 3H. CH 3 =CH of one isomer), 6.10 (q, IH. CH=CH 3 of one isomer), 6.66 (d, IH, NH of one isomer). 6.73 (d, IH, NH of one isomer), 7.17 (q, IH, CH=CH 3 of one isomer), 8.86. 9.10, 9.39, 9.47. 9.60, 9.62 (6s. 6H, 6 meso-H's); UV-Vis (CH 2 C1 2 (log ε)) λ max 414 (5.15), 506 (3.95), 542 (3.57), 628 (3.57), 680 (4.53), 686 (4.54) nm; MS (El) m e calc'd for C 38 H 47 N 5 O: 589.37805. found 589.37825; 589 (M + , 100 %); Analysis calc'd for C 38 H 47 N 5 O: C. 77.38; H. 8.03; N. 11.87; found: C. 77.10; H, 7.96; N, 1 1.59; Singlet Oxygen Test: Positive. (Ni-108) Nickel unsaturated lactam (mixture of isomers) The nickel N-hydroxymethylamide (Ni-106) ( 14 mg, 0.021 mmol) was dissolved in dichloromethane (5 mL) and boron trifluoride etherate (1 drop) was added. The colour changed from grey-green to bright green almost immediately. The solution was stined for 3 hours, then the solvent was evaporated and the residue purified by chromatography (silica, eluent 10 % ethyl acetate in dichloromethane) to give 4 mg pure less polar isomer and 8 mg of a mixture of the two isomers. R F 0.18 and 0.08 (silica - CH 2 C1 2 / 5 % AcOEt); UV-Vis (CH 2 C1 2 ) λ max 414, 506, 562, 644 nm; MS (El) m/e 645 (M + , 100 %), 616 (M + -29, 61 %). (I l l) Lactam reduction product 57 The lactam ( 108) (5 mg, 0.008 mmol) was dissolved in THF (3 mL) and triethylamine (1 drop). 10 % Pd on charcoal (2 mg) was added and the mixture was stiπed overnight under a hydrogen balloon. The catalyst was filtered off and air was bubbled through the solution for 1 hour. The THF was evaporated and the residue purified by chromatography (silica, eluent 20 % ethyl acetate in dichloromethane) to give the desired product as a dark green solid (2.5 mg, 50 % yield). m.p. >300°C; R F 0.44 (silica - CH 2 C1 2 / 20 % AcOEt); Η NMR (400 MHz, CDC1 3 ) δ -0.66 (br s, 2H, 2 x NH), -0.22 (t, J=7.5 Hz, 3H, CH 3 of angular ethyl group), 1.47-2.10 (m, 21H, 7 x CH 3 ), 2.60-2.75 (m, IH. CH of angular ethyl group), 3.04-3.20 (m. IH, CH of angular ethyl group), 3.50-4.00 (m. 13H). 4.05-4.25 (m. 3H), 4.63 (dd. J=12.5. 2.0 Hz. IH. angular H). 6.70 (d. J=5.22 Hz, IH, NH). 8.47 (s, I H. 1 meso-H), 9.38 (s, IH. 1 meso-H). 9.57 (s. IH. 1 meso-H); UV-Vis (CHC1 3 (log ε)) λmax ^06 (5.19), 502 (4.04), 534 (3.61 ), 670 (4.64) nm; MS (El) m/e calc'd for C 38 H 4 9N 5 0: 591.39374, found 591.39285; 591 (M + , 100 %). (1 13) 5-Formamidooctaethylpoφhyrin This compound was prepared in 87 % yield according to the procedure of Clezy et al. 207 m.p. 231-234 ° C; R F 0.14 (silica - CH 2 C1 2 ); 'H NMR (200 MHz. CDC1 3 ) δ - 3.43 (br s, 2H. 2 x NH), 1.70 (t, J=8 Hz. 6H, 2 x CH 3 ), 1.80-2.01 (m, 18H, 6 x CH3), 3.95-4.22 (m, 16H, 8 x CH 2 ), 8.61 (d, J=12 Hz, IH, CHO), 9.36 (br d. J=12 Hz. IH, NH), 10.02 (s, IH, 1 meso-H), 10.18 (s, 2H, 2 meso-H's); 13 C NMR (50 MHz, CDCl 3 ) δ 17.50, 18.55, 19.82. 21.77, 96.84, 97.48, 102.81 , 109.07, 140.57. 141.82, 142.55. 143.55, 143.75, 144.51 , 145.74, 167.82; UV-Vis (CH 2 C1 2 ) λ max 404. 504, 538. 572, 626 nm. (1 12) 5-Isocyanooctaethylpoφhyrin The formamide (1 13) (23 mg, 0.04 mmol) was dissolved in dry pyridine ( 5 58 mL). Phosphoryl chloride (200 μL, 2.1 mmol) was added dropwise under nitrogen, and the mixture was stiπed under nitrogen at 40°C for 2 hours. The solvent was removed in vacuo. and the residue purified by chromatography (flash silica, dichloromethane eluent) to give the product (20 mg, 90 %). m.p. 257-259°C; R F 0.39 (silica - 1 : 1 CH 2 Cl 2 :hexanes); ] H NMR (200 MHz, CDC1 3 ) δ -3.41 (v br s, 2H. 2 x NH), 1.82-2.00 (m, 24H. 8 x CH 3 ), 3.95-4.17 (m, 12H, 6 x CH 2 ), 4.28 (q, J=8 Hz. 4H, 2 x CH 2 ), 10.01 (s, I H, 1 meso-H), 10.14 (s, 2H, 2 meso-H's); 13 C NMR (50 MHz. CDC1 3 ) δ 17.22, 18.48. 19.70, 21.60, 97.96, 102.83, 1 14.30, 140.56. 141.01, 141.46. 141.92, 142.45, 143.76. 144.86, 145.38. 175.30; UV- Vis (CH 2 C1 2 ) λ max 408. 510. 546. 580. 634 nm. (1 16) 5-(C-Hydroxymethylformamido)octaethylpoφhyrin Paraformaldehvde ( 100 mg) was placed in a 25 mL flask equipped with a septum. Toluene (5 mL) was placed in another 25 mL flask equipped with a septum and a stir bar. The 2 flasks were connected by a canula and the flask containing toluene was also furnished with an empty balloon. The paraformaldehyde-containing flask was heated with a heat-gun and the resultant gaseous formaldehyde was bubbled through the toluene. The heating was continued for 5 min, after which the canula was removed, and the balloon filled with nitrogen. Boron trifluoride etherate (30 μL) was added dropwise by syringe and the mixture was stiπed for 5 min. The isocyanopoφhyrin (1 12) (17 mg, 0.030 mmol) was added to the reaction mixture and stirring continued under nitrogen for 30 min. The mixture was poured into water, extracted with dichloromethane, the extracts dried over sodium sulfate and the solvent evaporated in vacuo. The residue was purified by flash chromatography (silica, eluent 59 5 % ethyl acetate in dichloromethane) to give the polar pink product (5 mg, 28 % yield). m.p. 272-280 ° C; R F 0.09 (silica - CH C1 2 / 5 % AcOEt); Η NMR (200 MHz, CDC1 3 + 1 drop TFA) δ 1.12 (t. J=7.5 Hz, 6H. 2 x CH 3 ). 1.39 (t, J=7.5 Hz, 6H. 2 x CH 3 ), 1.60-1.80 (m. 12H, 4 x CH 3 ), 3.39-3.68 (m. 4H. 2 x CH 2 ), 3.79 (q, J=7.5 Hz. 4H, 2 x CH 2 ), 3.98 (q, J=7.5 Hz. 8H, 4 x CH 2 ), 4.76 (s. 2H. CH 2 OH), 10.10 (s, IH, 1 meso-H), 10.26 (s, 2H, 2 meso-H's); UV-Vis (CH 2 C1 2 ) λ max 404, 504, 538, 572. 624 nm; MS (El) m/e 607 (M + , 100 %), 576 (M + -CH 2 OH. 72 %). (Ni-1 16) [5-(C-Hydroxymethylformamido)octaethylpoφhyrinato]nickel(II) The C-hydroxymethylformamide ( 1 16) (5 mg. 0.008 mmol) was dissolved in dimethylformamide ( 1 mL) and nickel acetate tetrahydrate (30 mg, 0.12 mmol) was added. The mixture was refluxed for 2 hours, then poured into water, extracted with ethyl acetate, washed with water, dried over sodium sulfate and the solvent removed in vacuo. The residue was purified by flash chromatography (silica, eluent 5 % ethyl acetate in dichloromethane) to give the product (4 mg, 73 % yield). R F 0.23 (silica - CH 2 C1 2 / 10 % AcOEt): UV-Vis (CH C1 ) λ max 402. 526. 562 nm: MS (El) m/e 663 (M + . 100 %). (Ni-1 13) [5-Formamidooctaethylpoφhyrinato]nickel(II) The formamide (1 13) (32 mg, 0.055 mmol) was dissolved in DMF (5 mL), and nickel acetate tetrahydrate (20 mg, 0.080 mmol) was added. The mixture was refluxed for 1 hour, then allowed to cool, poured into water, and extracted 3 times with ethyl acetate. The organic phase was dried, the solvent was evaporated, and the residue was purified by chromatography (silica, eluent 5 % ethyl acetate in dichloromethane), to give the metallated product (27 mg, 77 % yield). m.p. 264-267 ; R F 0.27 (silica - CH 2 C1 2 ); Η NMR (200 MHz. CDC1 3 ) δ 1.61-1.86 (m. 24H. 8 x CH 3 ), 3.82 (q, J=7.5 Hz. 16H. 8 x CH ), 7.68 (d, J=12 Hz. IH, CHO), 9.13 (br d. J=12 Hz. IH. NH), 9.51 (s. IH. 1 meso-H), 9.55 (s, 2H. 2 meso- 60 RE RULE 91 H's); 13 C NMR (50 MHz. CDC1 3 ) δ 17.42. 18.16. 18.21. 18.26, 19.51. 19.62. 22.04. 96.75. 97.25. 107.44, 138.32. 139.20. 140.31, 141.05. 142.80. 143.60, 143.76. 145.72. 167.60; UV-Vis (CHCI3 (log ε)) λ max 400. 524. 562 nm. (Ni-1 12) (5-Isocyanooctaethylpoφhyrinato)nickel(II) The nickel formamide (Ni-1 13) (22 mg. 0.035 mmol) was dissolved in pyridine (2 mL), and phosphoryl chloride (4 drops) was added to this solution. The mixture was stiπed at room temperature for 30 min, then was added to water and the resulting solid was filtered off and washed with water, to give quantitative product. m.p. 282-284°C; R F 0.53 (silica - 1 : 1 CH 2 Cl 2 :hexanes); l H NMR (200 MHz. CDCI3) δ 1.58- 1.83 (m. 24H. 8 x CH3), 3.62-3.83 (m. 12H. 6 x CH 2 ), 3.99 (q, J=7.5 Hz. 4H. 2 x CH 2 ), 9.38 (s, IH. 1 meso-H), 9.41 (s. 2H. 2 meso-H's): 1 3 C NMR (50 MHz. CDCI3) δ 17.31, 18.27. 20.48. 22.08. 97.63. 97.79. 102.86. 136.10, 138.36. 140.78, 143.27, 143.59, 144.06. 146.12; UV-Vis (CH 2 C1 2 ) λ max 406. 536. 576 nm. (Ni-120) Nickel dimer The nickel isocyanide (Ni-1 12) (25 mg, 0.039 mmol) was dissolved in dichloromethane (3 mL), and boron trifluoride etherate (25 μL) was added. The mixture was stiπed overnight, then pyridine ( 1 drop) was added and the solvent was evaporated. The residue was purified by chromatography (silica, eluent dichloromethane: hexanes 1 : 1 ), then suspended in methanol and filtered, to give the green solid product ( 14 mg, 56 % yield). m.p. >300 ° C; R F 0.64 (silica - 1 : 1 CH 2 Cl 2 :hexanes); 'H NMR (400 MHz. 61 RECTIFIED S ET RULE 91 CDCI3) δ -0.05 (t. J=7.5 Hz. 12H. 4 x CH 3 of gem-diEt), 1.40-1.60 (m. 36H, 12 x CH3), 2.64-2.76 (m, 4H, 2 x CH 2 ), 3.10-3.30 (m, 16H. 8 x CH 2 ), 3.42 (q, J=7.5 Hz. 4H. 2 x CH 2 ), 3.64-3.76 (m, 4H. 2 x CH 2 ), 4.17 (q, J=7.3 Hz. 4H. 2 x CH 2 ), 7.64 (br s. 2H. 2 meso-H's), 7.96 (s, 2H. 2 meso-H's), 8.49 (br s. 2H, 2 meso-H's); UV-Vis (CHCI3 (log ε)) λ max 408 (4.69). 500 (4.99), 680 (4.64), 816 (4.13) nm; MS (FAB (3- NBA + CHCI3 matrix)) m/e calc'd for C 74 H 8 7Nιo 58 Ni 60 Ni: 1233.5757, found 1233.57831 ; 1233 (M + + 1 , 42 %); Analysis calc'd for C 7 4H 86 NιoΗCl: C, 70.02; H, 6.91 : N, 11.03; found: C, 69.98: H, 6.85: N, 10.93; Singlet Oxygen Test: Negative. (120) Free base dimer The nickel dimer (Ni-120) (5 mg. 0.004 mmol) was dissolved in concentrated sulfuric acid ( 1 mL). The solution was stiπed at room temperature for 2 hours, then poured into water, and extracted 3 times with dichloromethane. The solvent was dried and evaporated, and the residue was suspended in methanol and filtered to give the product as a grey solid (3.5 mg, 77 % yield). m.p. >300°C; R F 0.33 (silica - CH 2 Cl 2 :hexanes 1 : 1 ); 'H NMR (400 MHz. CDCI3) δ 0.04 (t. J=7.5 Hz. 12H. 4 x CH 3 of gem-diEt), 1.48-1.80 (m. 36H. 12 x CH3), 2.88-3.02 (m, 4H. 2 x CH 2 ), 3.23-3.46 (m, 16H. 8 x CH 2 ), 3.67 (q, J=7.5 Hz. 4H. 2 x CH ), 4.00-4.23 (m. 4H. 2 x CH 2 ), 4.63 (q, J=7.5 Hz. 4H. 2 x CH 2 ). 4.97 (br s, 2H), 5.82 (br s, 2H), 7.69 (s. 2H, 2 meso-H's), 7.88 (s, 2H, 2 meso-H's), 8.67 (s. 2H, 2 meso-H's); UV-Vis (CH 2 C1 2 ) λ max 400, 500, 572, 620, 678, 686, 742, 814 nm; MS (FAB (3-NBA + CHC1 3 matrix)) /e calc'd for C74H91N10: 1 1 19.74262, found 1 1 19.74164; 1 120 (M + +1 , 17 %); Singlet Oxygen Test: Positive. (Zn-120) Zinc dimer The free base dimer (120) (5 mg, 0.004 mmol) was dissolved in chloroform (5 mL) and zinc acetate dihydrate (10 mg, 0.046 mmol) in methanol (1 mL) was added. The mixture was refluxed 1 hour, then the solvent was evaporated, the residue was suspended in methanol. and this suspension was filtered to give quantitative dark 62 green solid product. m.p. >300°C; R F 0.32 (silica - CH 2 C1 2 ); Η NMR (200 MHz. CDCh) δ 0.02 (t, J=7.5 Hz, 12H, 4 x CH 3 of gem-diEt), 1.46-1.88 (m. 36H, 12 x CH 3 ), 2.84-3.06 (m. 4H. 2 x CH ), 3.32-3.58 (m. 16H. 8 x CH 2 ), 3.68 (q, J=7.5 Hz. 4H. 2 x CH 2 ), 4.01-4.24 (m, 4H. 2 x CH 2 ), 4.67 (q, J=7.5 Hz, 4H. 2 x CH 2 ), 7.88 (s. 2H. 2 meso- H's), 8.23 (s, 2H. 2 meso-H's), 8.91 (s, 2H. 2 meso-H's); UV-Vis (CH 2 C1 2 ) λ max 392, 466, 494, 518. 628. 680, 688, 728, 818 nm; MS (FAB (thioglycerol matrix)) m/e 1248 (M + . 100 %). (Ni-121) Nickel ethylidene dimer Formed in low yield as a side-product during the synthesis of (Ni-120). R F 0.39 (silica - CH 2 Cl 2 :hexanes 1 : 1); 'H NMR (400 MHz. CDCh) δ (selected resonances), 0.27 (t, 3H. CH 3 of one gem-diEt group), 0.91 (t. 3H. CH3 of one gem-diEt group), 2.82 (d, 3H, CH 3 =CH), 5.46 (s, IH. angular CH), 8.33 (q, I H, CH=CH 3 ), 8.84, 8.99, 9.03, 9.38, 9.42, 9.46 (6s, 6H, 6 meso-H's); UV-Vis (CH 2 C1 2 ) λmax 410, 500, 626 nm; MS (FAB (matrix 3-NBA + CHC1 3 )) m/e calc'd for C 7 4H 8 6Nιo 58 Ni 6( >Ni: 1232.61802, found 1232.57458: 1233 (M\ 2 %). (Zn-92) (5-Formylmethyliminooctaethylpoφhyrinato)zinc(II) The zinc aminopoφhyrin (Zn-91) (100 mg, 0.16 mmol) was dissolved in THF (5 mL) and a solution of glyoxal trimer dihydrate (125 mg, 0.59 mmol) in ethanol (5 mL) was added. The mixture was refluxed for 8 hours, then the solvent was evaporated and the residue purified by chromatography (silica, eluent 63 E 91 dichloromethane) to give the product (73 mg, 70 % yield). R F 0.74 (silica - CH 2 C1 2 ); 'H NMR (200 MHz. CDC1 3 ) δ 1.60-2.00 (m, 24H, 8 x CH 3 ), 3.80-4.25 (m. 16H, 8 x CH 2 ), 7.57 (d, J=8.1 Hz. IH. CH=CHO), 9.77 (s, I H, 1 meso-H), 9.91 (s, 2H. 2 meso-H's), 10.30 (d. J=8.1 Hz. IH, CHO); UV-Vis (CH 2 C1 ) λ max 408. 468, 544. 612 nm: MS (El) m/e 651 (M + , 100 %). (Zn-123) [Octaethyl-(3-hydroxypyrido)chlorinato]zinc(II) The zinc formylmethylimine (Zn-92) (93 mg, 0.14 mmol) was dissolved in toluene ( 10 mL) and activated Montmorillonite clay (50 mg) was added. The mixture was refluxed for 72 hours, then filtered and the solvent evaporated. The residue was purified by chromatography (silica, eluent initially dichloromethane. increasing polarity to 5 % methanol in dichloromethane) to give unreacted starting material (59 mg) and the slightly impure product (20 mg. 22 % yield). R F 0.58 (silica - 10 % AcOEt / CH 2 C1 2 ); > H NMR (200 MHz. pyridine-ds) δ 0.33 (t, J=7.1 Hz, 6H, 2 x CH3 of gem-diEt), 1.58-2.01 (m. 18H, 6 x CH 3 ), 2.62-2.82 (m, 2H, 2 x CH of gem-diEt CH 2 ), 3.43-3.92 (m, 12H, 6 x CH 2 ), 4.45 (q, J=7.6 Hz, 2H, 2 x CH of gem-diEt CH 2 ), 8.23, (s, I H. CH of pyridine ring), 9.15 (s, IH, 1 meso-H), 9.60 (s, IH, 1 meso-H) 9.62 (s, IH. 1 meso-H); UV-Vis (CH 2 C1 2 ) λ max 410, 422, 518, 558, 574, 624, 678 nm; MS (El) m/e 651 (M + , 100 %); Singlet Oxygen Test: Negative. (123) Octaethyl-(3-hydroxypyrido)chlorin The zinc chlorin (Zn-123) (20 mg, 0.030 mmol ) was dissolved in dichloromethane (5 mL) and TFA (2 drops) was added. The solution was stined for 2 64 RECTIFIE T RULE 91 hours, then pyridine (5 drops) was added to neutralize and the solvent was evaporated. The residue was chromatographed (silica, eluent 5 % methanol in dichloromethane) to give the product (6 mg, 35 % yield). R F 0.70 (silica - 10 % AcOEt / CH C1 ); 'H NMR (400 MHz. pyridine-d 5 ) δ 0.40 (t. J=7.3 Hz, 6H, 2 x CH 3 of gem-diEt), 1.63-1.81 (m, 15H. 5 x CH 3 ), 1.88 (t, J=7.3 Hz. 3H. 1 x CH 3 ), 2.78-2.91 (m. 2H. CH 2 ), 3.49-3.71 (m, 10H. 5 x CH 2 ), 3.85 (q, J=7.5 Hz, 2H, 2 x CH of gem-diEt CH 2 ), 4.43 (q, J=7.3 Hz, 2H, 2 x CH of gem- diEt CH 2 ), 8.40. 8.99, 9.58, 9.69 (4s, 3 meso-H's and CH=N) 13.06 (br s, IH. OH); UV-Vis (CHC1 3 (log ε)) λ max 412 (4.89), 490 (3.51), 522 (3.73), 556 (3.75), 616 (3.86). 672 (4.36) nm; MS (El) m/e calc'd for C 38 H 4 7N 5 O: 589.37805. found 589.37791 : 589 (M + . 100 %). 560 ((M-29) + . 65 %); Analysis calc'd for C 38 H 4 7N5θ-0.5H O: C. 76.22; H. 8.08: N. 1 1.70; found: C. 75.96: H. 7.64; N. 1 1.67; Singlet Oxygen Test: Positive. 65

Claims: Claims 1. A compound according to the formula wherein. R la , R l b are independently H or alkyl; R 2 is OH. halogen, alkoxy. OCO-alkyl, sulfonate. sulfate. or phosphate: R ja is H. or a phenyl or other aryl or heteroaryl group optionally substituted by one or more groups, each independently selected from halogen, hydroxy. alkyl. alkoxy. cyano. and ester; R 3 is H. halogen, formyl, nitro. amino or cyano; R 4 is H or alkyl; M is a poφhyrin-complexing metal, typically known in the art, or represents 2H; and N, the nitrogen atom of the pyridine ring, is optionally in the form of an N-oxide. or a salt such as an alkyl or hydrogen halide. 2. A pharmaceutical composition comprising a compound according to claim 1 and at least one substance that faciliates the therapeutic application thereof. 3. A compound selected from the group consisting of: 66 RECTIFIED SHEET RULE 91) β-isomer γ-isomβr 4. A compound selected from the group consisting of: 67 RECTIFIED SHEET RULE 91)

Inventors: DOLPHIN DAVID, JOHNSON CLAIRE K, DOLPHIN, DAVID, JOHNSON, CLAIRE, K.
Applicants: DOLPHIN DAVID, JOHNSON CLAIRE K, UNIV BRITISH COLUMBIA, DOLPHIN, DAVID, JOHNSON, CLAIRE, K., THE UNIVERSITY OF BRITISH COLUMBIA
Application year: 1998
Main classification: C07D471
IPC codes: C07D471