NIMESULIDE TOPICAL FORMULATIONS IN THE FORM OF LIQUID CRYSTALS

Abstract: The present invention relates to nimesulide topical formulations in the form of liquid crystals, in particular to those containing, inter alia, the emulsifier cetylstearyl glycoside, constituents of the lipid phase such as caprylic/capric triglycerids and jojoba oil, consistence factors such as cetylstearyl alcohol, and to the process for the preparation thereof. Said formulations have advantages such as higher stability, better release and absorption of nimesulide, a higher bioavailability of the active ingredient.

Description: NIMESULIDE TOPICAL FORMULATIONS IN THE FORM OF LIQUID CRYSTALS The present invention relates to nimesulide topical formulations in the form of liquid crystals. Nimesulide is a known antiinflam atory agent whose therapeutical efficacy has been proved for some time, but which has the drawback of having unfavourable chemical-physical characteristics; the main obstacle to the use of nimesulide in topical formulations is in fact its insolubility in water and, on the other hand, its poor solubility in the solvents/raw materials usually employed in such formulations. Some formulations of nimesulide for the external use are described in WO 96/11002; said formulations consist in dispersions of particles of the active ingredient throughout a component which, in the case of creams, comprises a hydrophilic polymer, an oily substance, a surfactant agent, a basic substance and water . The application does not mention any possible use of liquid crystals for nimesulide topical formulations. It has now been found that the emulsions containing liquid crystals, which are the object of the present invention, can be successfully used in nimesulide formulations for the topical use; liquid crystals present at the oil/water interface exert in fact a double effect in that they stabilize the emulsified system and control the release mechanism of the active ingredient, and therefore its bioavailability , thanks to the formation of a network system throughout which the active ingredient is homogeneously dispersed, thus involving unquestionable advantages compared with the conventional formulations containing water-in-oil (W/O) or oil-in-water (O/W) emulsions. For the formulations of the present invention cetylstearyl glycoside has been used as a non ionic oil in water (0/W) emulsifier, which, in the presence of a suitable aqueous phase, produces gel-structures by formation of liquid crystals. Cetylstearyl glycoside has safety and dermal tolerance characteristics ascribable to the natural origin of the compound itself; its hydrophilic portion derives, in fact, from the strong hydrolysis of starch and its lipophilic portion results from the hydrogenation of fatty acids derivatives of vegetable lipids. Cetylstearyl glycoside can be used at a concentration ranging from 3% to 10% by weight, preferably from 3% to 5%. The lipid phase of the emulsion consists of a caprylic/capric triglycerid, having a high carrier ability, thus giving the system sostantivity and emollient properties; caprylic/capric triglycerid can be used at a concentration ranging from 2% to 15% by weight, preferably from 2% to 6%. Moreover, the lipid phase preferably contains a liquid natural wax, namely the jojoba oil, which, besides enhancing nimesulide dispersion capability, provides very good properties of smoothness, flowability, emollient properties, pleasantness and agreeableness of use. The jojoba oil can be used at a concentration ranging from 1% to 15% by weight, preferably from 3% to 7%. The emulsion also comprises as a "consistence factor", cetylstearyl alcohol, which supplements the action of the cetylstearyl glycoside, due to the affinity between the two alkyl cetylstearyl chains, and contributing to the formation of the crystalline structure of the emulsion. Cetylstearyl alcohol can be used at a concentration ranging from 2% to 10% by weight, preferably from 2% to 5%. The formulation of the present invention can also contain emollients/humectants , such as cetyl esters 1- 15% by weight, cholesterol 0.3-0.5% by weight, glycerin 1-30% by weight, isopropyl myristate 1-10% by weight, isopropyl palmitate 0.05-5,5% by weight, lecithin 1-20% by weight, lanolin alcohols 0.5-15% by weight, vaseline 4-95% by weight, soy lipids 1-20% by weight, antimicrobial preservatives, such as parabens mixtures, and chemical preservatives, for example antioxidants such as tocopherol and lecithin, dermal absorption enhancers, such as propylene glycol 5-50% by weight, 2-pyrrolidone 0.1-10% by weight, pyrrolidone derivatives. The amount of water in the formulations ranges from 40% to 95% by weight, whereas pH is preferably kept at about 5.5, for example by addition of citric acid, so as to warrant the chemical-physical stability of nimesulide . Nimesulide can be dispersed in a wide range of concentrations, preferably from 0 to 5% by weight. The formulations based on liquid crystals of the invention have the following advantages: improvement in the chemical-physical stability of the emulsion; homogeneous distribution of the active ingredient in the crystalline structure; control of the release and absorption of nimesulide; increase in nimesulide bioavailability; high compliance by the user. Said formulations can be prepared by means of a process comprising: - hot preparation of the emulsion containing the two hydrophilic and lipophilic phases and the consistence factors; separated preparation of the phase containing the active ingredient nimesulide, optionally jojoba oil, caprylic/capric triglycerid and optional preservatives; hot mixing of the two phases from the above steps, and subsequent cooling; control and adjustment of pH , preferably with citric acid. The compositions of the invention proved to be well tolerated in animals. The efficacy of the compositions of the invention has been tested using well-known pharmacological tests. The following examples further illustrate the invention. EXAMPLE 1 Phase Ingredient % w/w Phase A Purified water q.ε. Glycerin 3.00 Phase B Cetylstearyl glycoside 5.00 Cetyl alcohol 2.00 jojoba oil 2.00 Caprylic and capric triglycerid 1.20 Phase C jojoba oil 3.00 Caprylic and capric triglycerid 1.80 Nimesulide Parabens in phenoxy- ethanol 1.00 Tocopherol - Ascorbyl palmitate soy lecithin - citric acid 0.01 Phase D Citric acid 5% sol. q.s. The formulation was prepared at four nimesulide concentrations, i.e. 2%, 3%, 4%, 5% w/w, each concentration in 3 different 6 kg batches. For the preparation, a Mambretti 25 kg turbodif fuser with cooling/heating water jacket, turbine, blades and vacuum was used. The A and C phases were mixed separately in a blade mixer, with heating. A mixture of methyl, ethyl and propyl p- hydroxybenzoates solubilized in phenoxyethanol and propylene glycol was used as preservative. EXAMPLE 2 % w/w Water 77.68 Cetylstearyl glycoside 5.00 Jojoba esters 5.00 Caprylic/capric triglycerid 3.00 Glycerin 3.00 Cetyl alcohol 2.00 Nimesulide 3.00 Phenoxyethanol and methyl, ethyl, propyl parabens 1.00 Perfluoropolymethy1isopropyl ether 0.30 Tocopherol - ascorbyl pal itate - lecithin - citric acid 0.01 Chemical- At After 45 days After 45 days physical- preparaat room temp. at temp. = 40°C characte- tion ristics Aspect Emulsion Emulsion Emulsion compact compact compact slightly slightly slightly yellowish yellowish yellowish P H 5.13 5.00 4.92 No traces of separation or decrease in viscosity were observed. EXAMPLE 3 % w/w Water 77.98 Cetylstearyl glycoside 5.00 Jojoba esters 5.00 Caprylic/capric triglycerid 3.00 Glycerin 3.00 Cetyl alcohol 2.00 Nimesulide 3.00 Phenoxyethanol and methyl, ethyl, propyl parabens 1.00 Tocopherol - ascorbyl palmitate lecithin - citric acid 0.01 Citric acid 5% sol. 0.0066 No traces of separation or decrease in viscosity were observed.

Claims: CLAIMS 1. Nimesulide topical formulations in the form of liquid crystals comprising: - a lipid phase containing alone or in combination thereof caprylic/capric triglycerids and optionally jojoba oil; an aqueous phase; cetylstearyl glycoside; - cetylstearyl alcohol. 2. Formulations according to claim 1 wherein said triglycerids of the lipid phase are present at a concentration ranging from 2% to 15% by weight. 3. Formulations according to claim 2 in which said concentration ranges from 2% to 6% by weight. 4. Formulations according to claim 1 wherein the jojoba oil is present at a concentration ranging from 1% to 15% by weight. 5. Formulations according to claim 1 wherein the cetylstearyl glycoside is present at a concentration ranging from 3% to 10% by weight. 6. Formulations according to claim 5 in which said concentration ranges from 3% to 5% by weight. 7. Formulations according to claim 1 wherein cetylstearyl alcohol is present at a concentration ranging from 2% to 10% by weight. 8. Formulations according to claim 7 in which said concentration ranges from 2% to 5% by weight. 9. Formulations according to any of the above claims, further comprising additives selected from emollients, humectants, absorption enhancers, preservatives. 10. Formulations according to claim 9 in which the wetting agent is glycerin. 11. Formulations according to claim 9 in which the preservatives are mixtures of parabens, tocopherol and " 5 lecithin. 12. Formulations according to claims 1-11 in which the active ingredient nimesulide is present at a concentration ranging from 0% to 5% by weight. 13. A process for the preparation of the formulations 0 of claims 1-12, which comprises: preparation of the emulsion containing the two hydrophilic and lipophilic phases and cetylstearyl glycoside; separated preparation of the phase containing the 5 active ingredient nimesulide, optionally jojoba oil, caprylic/capric triglycerid and optional preservatives; mixing of the two phases from the above steps; control and adjustment of pH , preferably with 0 citric acid.

Inventors: BADER STEFANO, HAEUSERMANN ENRIQUE, MONTI TIZIANA, BADER, STEFANO, HAEUSERMANN, ENRIQUE, MONTI, TIZIANA
Applicants: BADER STEFANO, HAEUSERMANN ENRIQUE, HELSINN HEALTHCARE SA, MONTI TIZIANA, BADER, STEFANO, HAEUSERMANN, ENRIQUE, HELSINN HEALTHCARE S.A., MONTI, TIZIANA
Application year: 1998
Main classification: A61K9
IPC codes: A61K9, A61K31