METHODS AND COMPOSITIONS FOR TREATING RHEUMATOID ARTHRITIS

Abstract: The present invention provides compositions and methods for the treatment of rheumatoid arthritis in a subject wherein one or more compounds of Formula (I) as defined herein alone or in combination with one or more other antiarthritic drugs provide suppression of rheumatoid arthritis.

Description: METHODS AND COMPOSITIONS FOR TREATING RHEUMATOID ARTHRITIS BACKGROUND OF THE INVENTION Rheumatoid arthritis is generally considered an autoimmune disease that is thought to be associated with activity of autoreactive T cells (See, e.g., Harris, E.D., Jr., The New England Journal of Medicine, 322 : 1277-1289 (1990)). Despite advances in treatment, rheumatoid arthritis remains a serious health problem. Although rarely fatal, arthritis is a major cause of morbidity, loss of time from work, lost productivity and decrease in quality of life. Rheumatoid arthritis causes severe pain and loss of joint mobility and can make accomplishing even simple tasks difficult. Current treatment methods and regimes for rheumatoid arthritis include administration of non-steroidal anti- inflammatory drugs such as acetylsalicylic acid (aspirin) , ibuprofen, naproxen and other such agents, gold compounds, penicillamine, methotrexate, cytotoxic agents (e.g., azothrioprine) , 4-aminoquinoline agents, and immunomodulators . However, improved treatments of rheumatoid arthritis, which can suppress or ameliorate symptoms such as inflammation, swelling, abnormal neovascularization, bone erosion, or cartilage erosion are needed. Preferably, such an improved method of treatment should be able to be combined with other treatment methods, should work rapidly to cause regression or stabilization of symptoms, and should be well tolerated. Preferably, such a treatment regime should also be useful in prophylaxis in susceptible individuals . SUMMARY OF THE INVENTION This invention relates to Dolastatin-15 derivatives, their preparation and use in the treatment of rheumatoid arthritis, in a mammal, for example, a human. The Dolastatin-15 derivatives of the present invention are compounds of Formula I : R 1 R 2 N-CHX-CO-A-B-D- (E) s - (F) t - (G) u -K (I) Formula I is discussed in detail below. Some examples of compounds of Formula I are specifically presented herein. For example, compounds of Formula I can be those in which R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec- butyl or tert -butyl; s is 1; t and u are each 0; A is valyl , isoleucyl or 2-tert-butylglycyl ; B is N-methylvalyl , 1-isoleucyl or 2-tert-butylglycyl; D is thiazolidinyl- carbonyl, 3 , 4 -dehydroprolyl or prolyl ; E is prolyl, thiazolidinyl-4-carbonyl , homoprolyl, hydroxyprolyl or 3,4- dehydroprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 , wherein R 5 is hydrogen or C^C-alkoxy and R 6 is a monovalent radical such as (1)- or (2)- adamantyl; (CH 2 ) v-phenyl with v=l; α, -dimethylbenzyl; a Cτ-Cj .2 linear or branched hydroxyalkyl group, such as - C(CH 3 ) 2 -CH 2 -CH 2 -OH, also referred to as 3-hydroxy-l , 1- dimethylpropyl ; a C 3 -C 10 cycloalkyl group, such as bicyclo [3.3.0] octa-1-yl, 1-methylcyclopentyl or 1- SUBSTΓΓUTE SHEET (RULE 25) methylcyclohexyl; or a C ! -C 12 linear or branched alkyl group, such as -C(CH 3 ) 3 , also referred to as tert-butyl; -C-CH.-CH 3 , also referred to as 1, 1-dimethylpropyl ; (CΗ 3 ) 2 " -C (CH 2 -CH 3 ) 2 , also referred to as 1-methyl-1-ethylpropyl ; C_H 3 -CH-C(CH 3 ) 3 , also referred to as (S) - or (R) -1-methyl-2 , 2- CH 3 dimethyl-propyl; -CH-CH(CH 3 ) 2 , also referred to as (S) - or (R) -l-ethyl-2- C 2 H 5 methylpropyl ; -CH-CH (CH 3 ) 2 , also referred to as 1 -isopropyl-2 -methyl- CH(CH 3 ) 2 propyl; or -C(CH 3 ) 2 -CH(CH 3 ) 2 , also referred to as 1 , 1-dimethyl-2- methylpropyl ; -CH(CH 3 ) 2 , also referred to as isopropyl; -CH(CH 3 ) CH 2 CH 3 , sec-butyl [ (S) or (R) ] ; or -CH(CH 3 )CH(CH 3 ) 2 , also referred to as 1 , 2-dimethylpropyl . This invention also relates to methods for the treatment of rheumatoid arthritis, in a mammal, for example a human, in which one or more of the Dolastatin-15 derivatives described herein are used. In the method of the present invention, one or more of the Dolastatin-15 derivatives are administered, alone or in a phamacologically acceptable carrier, in a therapeutically effective amount to treat rheumatoid arthritis in a mammal having or susceptible to rheumatoid arthritis. In another aspect of the invention one or more Dolastatin-15 derivatives are administered in combination with one or more other antiarthritic drugs to a mammal having or susceptible to rheumatoid arthritis. In a specific embodiment, two or more Dolastatin-15 derivatives are administered alone or in combination with one or more other antiarthritic drugs to a mammal having or susceptible to rheumatoid arthritis. Administration of two or more Dolastatin-15 derivatives or administration of Dolastatin-15 derivative (s) in combination with one or more other antiarthritic drugs enhances treatment of rheumatoid arthritis. For example, a combination provides a greater suppression or fewer side effects, and/or can make it possible to administer a lower dose of the known antiarthritic drug to produce the same effect produced with a higher dose. The other antiarthritic drug can be, but is not limited to, one or more of the following: (1) a nonsteroidal anti-inflammatory agent such acetylsalicylic (aspirin) , ibuprofen, or naproxen; (2) an organic gold derivative such a gold sodium thiomalate, aurothioglucose, or auranofin; (3) D-pencillamine ; (4) a 4-aminoquinoline agent such as hydroxychloroquine ; (5) azathioprine; (6) methotrexate; (7) cyclosporin; (8) an angiogenesis inhibitor such as AGM-1470 (Ingber, et al . , Nature 348 , (1990) 555) ; (9) monoclonal antibodies to T cells; (10) monoclonal antibodies to adhesion molecules; (11) monoclonal antibodies to cytokines and growth factors; (12) Tumor Necrosis Factor Receptor (TNFR)-IgG ; (13) IL-1 receptor antagonists; and (14) ICE inhibitors. Also the subject of this invention are pharmaceutical compositions which comprise one or more Dolastatin-15 derivatives of Formula I either alone or in combination with one or more other antiarthritic drugs. The pharmaceutical composition can optionally include a pharmaceutically acceptable carrier, diluent or a compound which aids in processing, for example, binders, fillers and preservatives . BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 depicts compounds i-xvii, as examples of Dolastatin-15 derivatives having the structure of Formula I. Figure 2 is a graph showing mean arthritic score as a function of the number of days after immunization with type II collagen, for mice treated with saline (control) , dexamethasone (standard therapy) and compound ii from Figure 1. Treatment was commenced on day 26 post- immunization and was terminated on day 35 post- immunization. Figure 3 is a graph showing mean arthritic score as a function of the number of days after immunization with type II collagen, for mice treated with vehicle (control) , dexamethasone (standard therapy) and compound ii from Figure 1. Treatment was commenced on day 48 post immunization and lasted for 21 days. Figure 4 is a graph showing the degree of synovitis and cartilage damage as determined by histopathological analysis for mice treated with vehicle, dexamethasone (standard therapy) and compound ii of Figure 1. The mice were treated starting at 48 days after immunization with type II collagen and treatment lasted for 21 days. Necropsy was conducted on day 71 post-immunization. DETAILED DESCRIPTION OF THE INVENTION The present invention relates to Dolastatin-15 derivatives useful in the treatment of rheumatoid arthritis in a mammal. The Dolastatin-15 derivatives of the invention are compounds having the structure shown in Formula I, as further described below. The compound is ~ administered in a therapeutically effective amount. As used herein the term "therapeutically effective amount" refers to an amount sufficient to elicit the desired biological response. In this invention, the desired biological response of the treatment is suppression of rheumatoid arthritis. As used herein "suppression" includes any or all of the following: (1) amelioration of existing symptoms; (2) prevention or slowing of the progression of symptoms; (3) prevention or delay of the inception or occurrence of the disease in a susceptible subject, i.e., prophylaxis. Symptoms typically associated with rheumatoid arthritis, include but are not limited to, inflammation, swelling, abnormal neovascularization, bone erosion and cartilage erosion. One or more of these symptoms are suppressed when a therapeutically effective amount of a Dolastatin-15 derivative compound of Formula I is administered. COMPOUNDS OF FORMULA I A number of short peptides with significant activity as inhibitors of cell growth have been isolated from the Indian Ocean sea hare Dolabella auricularia (Bai, et al . , Biochem . Pharmacology, 40 : 1859-1864 (1990); Beckwith et al . , J. Natl . Cancer Inst . , 85 : 483-488 (1993) and references cited therein) . These include Dolastatins 1-10 (U.S. Patent No. 4,816,444, issued to Pettit et al . ) and Dolastatin-15 (European Patent Application No. 398558). Dolastatin-15 , for example, markedly inhibits the growth of the National Cancer Institute's P388 lymphocytic leukemia cell line, a strong predictor of efficacy against various types of human malignancies. This compound, however, is present only in trace quantities in the sea hare and is difficult to isolate, expensive to synthesize and suffers from poor aqueous solubility. The compounds of Formula I are derivatives of Dolastatin-15. It has been determined that, surprisingly, the compounds of Formula I are useful in a method for the treatment of rheumatoid arthritis. Dolastatin-15 derivatives of Formula I, which are employed in the method of the present invention, can be synthesized, as described herein and in related copending application U.S.S.N. 08/472,453, filed June 7, 1995, the teachings of which are incorporated herein in their entirety. The Dolastatin-15 derivatives of Formula I generally comprise L-amino acids, but they can also contain one or more D-amino acids, as described in related copending application U.S.S.N. 08/472,453 filed on June 7, 1995. The compounds of Formula I can also be present as salts with physiologically-compatible acids, such as, but not limited to, hydrochloric acid, citric acid, tartaric acid, lactic acid, phosphoric acid, methanesulfonic acid, acetic acid, formic acid, maleic acid, fumaric acid, malic acid, succinic acid, malonic acid, sulfuric acid, L-glutamic acid, L-aspartic acid, pyruvic acid, mucic acid, benzoic acid, glucuronic acid, oxalic acid, ascorbic acid and acetylglycine . For purposes of the present invention, the term "monovalent radical" is intended to mean an electrically neutral molecular fragment capable of forming one covalent bond with a second neutral molecular fragment . Monovalent radicals include the hydrogen atom, alkyl groups (e.g. methyl, ethyl, propyl and tert-butyl groups), cycloalkyl groups, hydroxy alkyl groups, adamantyl groups, halogen atoms (e.g. fluorine, chlorine and bromine atoms) , aryl groups (e.g. phenyl, benzyl and naphthyl groups) and alkoxy groups (e.g. methoxy and ethoxy groups) . Two monovalent radicals on adjacent sigma-bonded atoms can also form a pi bond between the adjacent atoms. Two monovalent radicals may also be linked together, for example, by a polymethylene unit to form a cyclic structure. For example, the unit -N(R)R', wherein R and R' are monovalent radicals, can, together with the nitrogen atom, form a heterocyclic ring. In addition, two monovalent radicals bonded to the same atom can also form a divalent radical, such as an alkylidene group, for example, a propylidene group, or an oxygen atom. More specifically, for the compounds of Formula I : R 1 is alkyl, such as C 1 -C 3 ; cycloalkyl, such as cyclopropyl; alkylsulfonyl, such as Cj-C;,; fluoroalkyl, such as fluoroethyl, difluoroethyl, fluoroisopropyl ; aminosulfonyl which may be substituted by alkyl, such as methyl; R 2 is hydrogen; alkyl, such as C 1 -C 3 ; fluoroalkyl, such as fluoroethyl, difluoroethyl, fluoroisopropyl; cycloalkyl, such as cyclopropyl; R x -N-R 2 together may be a pyrrolidino or piperidino residue; A is a valyl, isoleucyl, leucyl, allo-isoleucyl, 2, 2-dimethylglycyl, 2-cyclopropylglycyl, 2- cyclopentylglycyl, 3 -tert-butylalanyl, 2-tert- butylglycyl, 3-cyclohexylalanyl, 2-ethylglycyl, 2-cyclohexylglycyl, norleucyl or norvalyl residue; B is a N-alkyl-valyl, -norvalyl, -leucyl, -isoleucyl, -2-tert-butylglycyl, -3-tert- butylalanyl, -2-ethylglycyl, -2- cyclopropylglycyl, -2-cyclopentylglycyl, norleucyl or -2-cyclohexylglycyl residue where N- alkyl is preferably N-methyl or N-ethyl; D is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl, 4 -fluoroprolyl, 3 -methylprolyl , 4- methylprolyl, 5 -methylprolyl , azetidine-2- carbonyl, 3 , 3-dimethylprolyl , 4 , 4 -difluoroprolyl , oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue; E is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl , 4 -fluoroprolyl, 3 -methylprolyl, 4- methylprolyl, 5 -methylprolyl, azetidine-2- carbonyl, 3 , 3-dimethylprolyl, 4 , 4 -difluoroprolyl, oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue; F and G are independently selected from the group consisting of prolyl, homoprolyl, hydroxyprolyl, thiazolidinyl-4 -carbonyl , 1 -aminopentyl-1- carbonyl, valyl, 2-tert-butylglycyl, isoleucyl, leucyl, 3-cyclohexylalanyl, phenylalanyl, N- methylphenylalanyl , tetrahydrosioquinolyl-2 - histidyl, 1-aminoindyl-l-carbonyl, 3- pyridylalanyl, 2-cyclohexylglycyl , norleucyl, norvalyl, neopentylglycyl , trytophanyl, glycyl, 2 , 2-dimethylglycyl alanyl, β-alanyl and 3- naphthylalanyl residues; X is hydrogen, alkyl (such as C^C,) , cycloalkyl (such as C 3 -C 7 ) , -CH 2 -cyclohexyl or arylalkyl (such as benzyl or phenethyl) ; s, t and u are independently 0 or 1; and K is hydroxy, alkoxy (such as CT-C,) , phenoxy, benzyloxy or a substituted or unsubstituted amino moiety. In addition, the compounds of Formula I can be present as a salt thereof with physiologically tolerated acids. One subclass of compounds of this invention includes compounds of Formula I wherein R x -N-R 2 is a pyrrolidinyl or piperidinyl residue. Another subclass of compounds of this invention includes compounds of Formula I wherein K is an amino moiety of the formula R 5 -N-R 6 , wherein: R 5 is hydrogen, or hydroxy, or C 1 . 1 alkoxy, or benzyloxy, or phenyloxy or C λ . 12 linear or branched hydroxyalkyl , such as 3 -hydroxy-1 , 1-dimethylpropyl , or C 1 _ 1 linear or branched alkyl (which may be substituted by one or more fluoro atoms), or C 3 _ 10 -cycloalkyl , such as, bicyclo [3.3.0] octa-lyl, 1-methylcyclopentyl or 1- methylcylcohexyl; or benzyl (which may be substituted by up to three substituents which may independently be CF 3/ nitro, C 1-7 alkylsulfonyl , C^ alkoxy, phenoxy, benzoxy, halogen, C^,,-alkyl, cyano, hydroxy, N(CH 3 ) 2 , COOMe, COOEt, COOiPr, or COONH 2 ) ; R 6 is hydrogen, or C 1 . 12 linear or branched alkyl (which may be substituted by one or more fluoro atoms) , or C^ ! , linear or branched hydroxyalkyl , such as 3- hydroxy-1, 1-dimethylpropyl, or C 3 _ 10 -cycloalkyl, such as bicyclo [3.3.0] octa-1-yl, or 1-methylcyclopentyl or 1-methylcyclohexyl; or - (CH 2 ) V -C 3 _ 7 - cycloalkyl (v=0,l,2, or 3), or norephedryl, or norpseudoephedryl , or quinolyl , or pyrazyl, or -CH 2 -benzimidazolyl, or (1) -adamantyl , or (2) -adamantyl- -CH 2 -adamantyl , or alpha-methyl-benzyl , or alpha-dimethylbenzyl, or - (CH 2 ) v -phenyl (v=0,l,2, or 3; which may be substituted by up to two substituents which may independently be CF 3 , nitro, C^ alkylsulfonyl, C 1-4 alkoxy, phenoxy, benzoxy, halogen, C 1-4 -alkyl which may form a cyclic system, cyano, hydroxy, N(CH 3 ) 2 , COOMe, COOEt, COOiPr, or COONH 2 ) , or - (CH 2 ) „,-naphthyl (m=0 or 1); or -(CH 2 ) W - benzhydryl (w=0,l, or 2); or biphenyl or picolyl or benzothiazolyl or benzoisothiazolyl or benzopyrazolyl or benzoxazolyl or - (CH 2 ) m -fluorenyl (m=0 or 1); or pyrimidyl or - (CH 2 )m-indanyl (m=0 or 1); or - (CH 2 CH 2 0) y -CH 3 (y=0,l,2,3,4, or 5), or -(CH 2 CH 2 0) y - CH 2 CH 3 (y=0,l,2,3,4, or 5), or NH-C 6 H 5 (which may be substituted by up to two substituents which may independently be CF 3 , nitro, C 1 _ 1 alkylsulfonyl, C 1 _ 4 alkoxy, halogen, Cj_ 4 alkyl which may form a cyclic system, cyano, hydroxy, COOMe, COOEt, COOiPr, or COONH 2 ) , or -NCH 3 -C 6 H 5 or -NH-CH 2 -C 6 H 5 or -NCH 3 -CH 2 -C 6 H 5 or 5-membered heteroaryl which may be substituted by up to two substituents which may independently be CF 3 , nitro, thio echyl, thioethyl, C 3 _ 6 -cycloalkyl, -CH 2 -CO0Ξt, C 3 . 4 -alkylene group forming a bicyclic system with the heterocycie, phenyl; or - CHR 7 -5-membered heteroaryl (which may be substituted by up to two substituents which may independently be CF 3 , nitro, cyano, halogen, COOMe, COOEt, COOiPr, CONH 2 , C^-alkyl, C,_ 4 -alkoxy, phenyl, benzyl, naphthyl, or C^ .-alkylsulfonyl [R 7 = hydrogen, linear or branched C 1-5 alkyl, benzyl; or R 7 and R 5 together form a group -(CH 2 ) 3 - or -(CH 2 ) 4 -) . This subclass includes compounds of Formula I wherein s, z and u are independently 0 or 1; R 1 , R 2 and X are lower alkyl, A is a lower alkyl amino acid, B is a N-loweralkylated lower alkyl amino acid; D,E,F,G and K are as previously defined. With the foregoing in mind, three sees of such compounds can thus be depicted by the following formulas II, III, and IV: R^N-CXH-CO-A-B-Pro-Pro-F-G-K II R^N-CXH-CO-A-B-Pro-Pro-F-K III R^N-CXH-CO-A-B-Pro-Pro-K IV -CHR 7 -5-membered heteroaryl may, for example, be represented by one of the following residues: ONE; CK 3 COOΞt COOMe -NR 5 CHR 7 - 5-membered heteroaryl may, for example, be represented by the following residues: 5-membered heteroaryl may, for example, be represented by the following residues: In another subclass of- compounds of this invention R 5 -N-R 6 together may form structures selected from the group consisting of: Still another subclass of compounds of this invention includes, for example, compounds of Formula I wherein s, t and u are 1 and K is a hydroxy, alkoxy, phenoxy or benzyloxy moiety. Yet another subclass of compounds of this invention includes, for example, compounds of Formula I wherein s and t are 1, u is 0 and K is a hydroxy, alkoxy, phenoxy or benzyloxy moiety. Another subclass of compounds of this invention includes, for example, compounds of Formula I wherein s is 1, t and u are 0 and K is a hydroxy, alkoxy, phenoxy or benzyloxy moiety. In particular embodiments, a compound of Formula I is one in which R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, isoleucyl or 2-tert-butylglycyl; B is N-methylvalyl, 1-isoleucyl or -2-tert-butylglycyl; D is prolyl, thiazolidinyl-4 -carbonyl or 3 , 4 dehydroprolyl; E is prolyl, thiazolidinyl- -carbonyl , homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted or unsubstituted amino moiety having the formula R 5 -N-R 6 . In a further embodiment, the Dolastatin-15 derivative is a compound of Formula I in which R 1 and R' are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, isoleucyl or 2-tert- butylglycyl; B is N-methylvalyl, 1- isoleucyl or 2- tertbutylglycyl ; D is prolyl, thiazolidinyl-4-carbonyl,- or 3 , 4 -dehydroprolyl; E is prolyl, thiazolidinyl-4 -carbonyl , homoprolyl, 3 , 4 -dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or Cj-C, alkoxy and R b is a C ± -C 12 linear or branched alkyl group or a C^C^ linear or branched hydroxyalkyl group represented, for example, by the following monovalent radicals : -C(CH 3 ) 2 -CH 2 -CH 2 -0H, also referred to as 3 -hydroxy-1 , 1- dimethylpropyl ; -C(CH 3 ) 3 , also referred to as tert-butyl; -CH 3 , also referred to as 1,1-dimethyl propyl; -C(CH 2 -CH 3 ) 2 also referred to as 1-methyl-l-ethyl propyl; CH 3 -CH-C(CH 3 ) 3 , also referred to as (S) - or (R) -l-methyl-2 , 2- CH 3 dimethyl propyl; -9H-CH(CH 3 ) 2 , also referred to as (S) - or (R) -l-ethyl-2- C 2 H 5 methyl propyl; - H-CH (CH 3 ) 2 , also referred to as 1- isopropyl -2 -methyl CH(CH 3 ) 2 butyl; or -C (CH 3 ) 2 -CH (CH 3 ) 2 , also referred to as 1 , 1-dimethyl-2 -methyl propyl -CH(CH 3 ) 2 , also referred to as isopropyl -CH(CH 3 )CH 2 CH 3 , also referred to as sec-butyl, (S) - or (R) - -CH(CH 3 )CH(CH 3 ) 2 , also referred to as 1, 2-dimethylpropyl . In another embodiment, the Dolastatin-15 derivative of the invention is a compound of Formula I in which R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, isoleucyl or 2-tert-butylglycyl; B is N-methylvalyl, 1- isoleucyl or 2-tert-butylglycyl; D is prolyl, thiazolidinyl-4 -carbonyl, 3 , 4 -dehydroprolyl ; E is prolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3 , 4 -dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or C^C, alkoxy and R 6 is a monovalent radical such as a C 3 -C 10 cycloalkyl group (e.g. cyclobutyl, cyclopentyl, cyclohexyl, or 1- methylcyclopentyl, or 1-methylcyclohexyl or bicyclo [3.3.0] octa-1-yl) ; a (1)- or (2) -adamantyl group; (CH 2 ) v-phenyl with v=l or , -dimethylbenzyl . In a further embodiment, the Dolastatin-15 derivative of the invention is a compound of Formula I in which R 1 and R 2 are each methyl; X is isopropyl; s is 1; t and u are each 0; A is valyl; B is N-methylvalyl; D is prolyl; E is prolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen and R 6 is a tert- butyl group. This compound corresponds to compound ii depicted in Figure 1. The results of the use of compound ii of Formula I, are described in Examples 3 and 4 and represented graphically in Figures 2, 3 and 4. The Dolastatin-15 derivative of the present invention can optionally be administered in a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers are well known to those who are skilled in the art. The choice of a carrier will be determined in part by the particular compound of Formula I, as well as by the particular method used to administer the Dolastatin-15 derivative . Also the subject of this invention are pharmaceutical composition which comprise one or more Dolastatin-15 derivatives of Formula I either alone or in combination with one or more other antiarthritic drugs, such as those described herein. The pharmaceutical composition can optionally include a pharmaceutically acceptable carrier, diluent or a compound which aids in processing, for example, binders, fillers and preservatives. In another aspect, the present invention comprises a method for the treatment of rheumatoid arthritis in a mammal using the Dolastatin-15 derivatives of Formula I. For purposes of this invention the phrases "method of treatment of rheumatoid arthritis" and "suppresion of rheumatoid arthritis" can be used interchangeably. As used herein, the term "suppression" includes any or all of the following: (1) amelioration of existing symptoms; (2) prevention or slowing of progression of symptoms; (3) prevention or delay of the inception or occurrence of the disease in a susceptible subject, i.e., prophylaxis. The method of treatment of the present invention comprises administering a therapeutically effective amount of one or more compounds of Formula I . The compounds of Formula I can be administered alone or with a pharmaceutically accepted carrier or diluent appropriate for the desired route of administration. Administration can be by any of the means which are conventional for pharmaceuticals, including oral and parenteral means such as subcutaneously, intravenously, intramuscularly, intraperitoneally, nasally or rectally. Such pharmaceutical compositions may also contain other therapeutically active ingredients. In another aspect of the invention one or more Dolastatin-15 derivatives are administered either alone or in combination with one or more other antiarthritic drugs in a mammal having or susceptible to rheumatoid arthritis. Administration of one or more Dolastatin-15 derivative (s) in combination with one or more other antiarthritic drugs enhances treatment of rheumatoid arthritis. For example, a combination provides greater suppression or fewer side effects, and/or can make it possible to administer a lower dose of the known antiarthritic drug to produce the same effect. The other antiarthritic drug can be, but is not limited to, the following: (1) a nonsteroidal anti- inflammatory agent such acetylsalicylic (aspirin) , ibuprofen, or naproxen; (2) an organic gold derivative such a gold sodium thiomalate, aurothioglucose, or auranofin; (3) D-pencillamine ; (4) a 4-aminoquinoline agent such as hydroxychloroquine; (5) azathioprine; (6) methotrexate ; (7) cyclosporin; (8) an angiogenesis inhibitor such as AGM-1470 (Ingber, et al . , Nature 348 , (1990) 555); (9) monoclonal antibodies to T cells; (10) monoclonal antibodies to adhesion molecules; (11) monoclonal antibodies to cytokines and growth factors; (12) Tumor Necrosis Factor Receptor (TNFR) -IgG; (13) IL-1 receptor antagonists; and ICE inhibitors. In a specific embodiment, at least two or more Dolastatin-15 derivatives are administered either alone or in combination with one or more other antiarthritic drugs to a mammal having or susceptible to rheumatoid arthritis. The dosage administered to the mammal, such as a human, includes a therapeutically effective amount of a compound of Formula I, as described herein. The dosage can be determined empirically, using known methods, and will depend upon factors such as the biological activity, mechanism of action, toxicity profile of the particular compounds employed; the means of administration; the age, health and body weight of the recipient; the nature duration and extent of the symptoms; the frequency of treatment; the administration of other therapies; and the effect desired. A typical daily dose of the compounds of Formula I will be from about 1 to about 100 milligrams per kilogram of body weight by oral administration and from about 1 to about 100 milligrams per kilogram of body weight by parenteral administration. The Dolastatin-15 derivatives of the present invention can be administered in conventional solid or liquid pharmaceutical forms, for example, uncoated or (film) coated tablets, capsules, powders, granules, suppositories or solutions. These are produced in a conventional manner. The active substances can for this purpose be processed with conventional pharmaceutical aids such as tablet binders, fillers, preservatives, tablet disintegrants, flow regulators, plasticizers , wetting agents, dispersants, emulsifiers, solvents, sustained release compositions, antioxidants and/or propellant gases (cf. H. Sucker et al . : Pharmazeutische Technologie, Thieme-Verlag, Stuttgart, 1978) . The administration forms obtained in this way typically contain from about 1 to about 90% by weight of the active substance. If more than one Dolastatin-15 derivative is administered, they can be administered at the same time (simultaneously) or at separate times (sequentially) , provided that they are administered in such an order and at intervals appropriate to produce the desired therapeutic effect. If two or more Dolastatin-15 derivatives are administered at the same time, they can be given separately (as individual derivatives) or in physical combination (as a mixture or combination) . The same is the case when one or more Dolastatin-15 derivatives are administered with one or more other antiarthritic drugs. They can be administered simultaneously or sequentially and individually or as a combination or mixture. Pharmaceutical compositions which include one or more Dolastatin-15 derivatives or one or more Dolastatin-15 derivatives and one or more other antiarthritic drugs are also the subject of this invention. The compounds of Formula I are described in detail above. In a particular embodiment, the method of the invention uses a Dolastatin-15 derivative of Formula I in which R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, isoleucyl or 2-tert-butylglycyl; B is N-methylvalyl, 1-isoleucyl or 2-tert-butylglycyl; D is prolyl, thiazolidinyl-4 -carbonyl or 3 , 4 -dehydroprolyl; E is prolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3 , 4 -dehydroprolyl or hydroxyprolyl; and K is a substituted or unsubstituted amino moiety having the formula R b -N-R 6 . In a further embodiment, the method of the invention uses a Dolastatin-15 derivative of Formula I in which R 1 and R" are each methyl or ethyl ; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, isoleucyl or 2-tert-butylglycyl; B is N-methylvalyl, 1- isoleucyl or 2-tert-butylglycyl; D is prolyl, thiazolidinyl-4-carbonyl or 3 , 4 -dehydroprolyl; E is prolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3 , 4 -dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or Cj- i alkoxy and R 6 is a C l -C 12 linear or branched alkyl group or C^C^ linear or branched hydroxyalkyl group represented, for example, by the following monovalent radicals: -C(CH 3 ) 2 -CH 2 -CH ? -OH, also referred to as 3 -hydroxy-1 , 1- dimethylpropyl ; -C(CH 3 ) 3 , also referred to as tert-butyl; -<^-CH.-CH 3 , also referred to as 1,1-dimethyl propyl; (CH 3 ) -C (CH 2 -CH 3 ) 2 , also referred to as 1-methyl-1-ethyl propyl; CH 3 -ς:H-C (CH 3 ) 3 , also referred to as (S) - or (R) -l-methyl-2 , 2- CH 3 dimethyl propyl ; -CH-CH(CH 3 ) 2 , also referred to as (S) - or (R) -l-ethyl-2- CH 2 H 5 methyl propyl ; -CjΗ-CH (CH 3 ) 2 , also referred to as 1- isopropyl-2 -methyl CH(CH 3 ) 2 " butyl; or -C(CH 3 ) 2 -CH(CH 3 ) 2 , also referred to as 1, 1-dimethyl-2 -methyl propyl -CH(CH 3 ) 2 also referred to as isopropyl -CH (CH 3 ) CH 2 CH 3 , also referred to as sec-butyl , ( S ) - or (R) - -CH(CH 3 ) CH(CH 3 ) 2 , also referred to as 1 , 2-dimethylpropyl . In another embodiment, the method of the invention uses a compound of Formula I in which R 1 and R' are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, isoleucyl or 2-tert- butylglycyl; B is N-methylvalyl, 1-isoleucyl or 2-tert- SUBSTΓΓUTE SHEET (RULE 26) butylglycyl; D is prolyl, thiazolidinyl-4 -carbonyl, 3,4- dehydroprolyl ; E is prolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3 , 4 -dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or Ci- , alkoxy and R 6 is a monovalent radical such as a C 3 -C 10 cycloalkyl group (e.g. cyclobutyl, cyclopentyl, cyclohexyl, l-methylcyclopentyl, 1- methylcyclohexyl or bicyclo [3.3.0] octa-1-yl) ; a (1)- or (2) -adamantyl group; (CH 2 ) v-phenyl with v=l or ,α- dimethylbenzyl . In a further embodiment, the method of the invention uses a Dolastatin-15 derivative of Formula I in which R 1 and R 2 are each methyl; X is isopropyl; s is 1; t and u are each 0; A is valyl; B is N-methylvalyl; D is prolyl; E is prolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen and R 6 is a tert- butyl group. This compound corresponds to compound ii depicted in the Figure 1. The use of compound ii in the treatment of rheumatoid arthritis is described in Examples 3 and 4 with results presented in Figures 2, 3 and 4. SYNTHETIC METHODS The compounds of Formula I can be prepared by known methods of peptide synthesis such as those described herein and, in U.S. Patent Application Serial No. 08/470,453 filed June 7, 1995, the teachings of which are incorporated herein by reference. The peptides can be assembled sequentially from individual amino acids or by linking suitable small peptide fragments. In sequential assembly, the peptide chain is extended stepwise, starting at the C- terminus, by one amino acid per step. In fragment coupling, fragments of different lengths can be linked together, and the fragments can also be obtained by sequential assembly from amino acids or by fragment coupling of still shorter peptides. In both sequential assembly and fragment coupling it is necessary to link the units by forming an amide linkage, which can be accomplished via a variety of enzymatic and chemical methods. The methods described herein for formation of peptidic amide linkages, are also suitable for the formation of non-peptidic amide linkages. Chemical methods for forming the amide linkage are described in detail in standard references on peptide chemistry, including Mύller, Methoden der organischen Chemie Vol. XV/2, 1-364, Thieme Verlag, Stuttgart, (1974); Stewart and Young, Solid Phase Peptide Synthesis. 31-34 and 71-82, Pierce Chemical Company, Rockford, IL (1984); Bodanszky et al . , Peptide Synthesis. 85-128, John Wiley & Sons, New York, (1976); Practice of Peptide Synthesis. M. Bodansky, A. Bodansky, Springer-Verlag, 1994 and other standard works in peptide chemistry. Preferred methods include the azide method, the symmetric and mixed anhydride method, the use of in si tu generated or preformed active esters, the use of urethane protected N-carboxy anhydrides of amino acids and the formation of the amide linkage using coupling reagents, such as dicyclohexylcarbodiimide (DCC) , diisopropylcarbodiimide (DIC) , l-ethoxycarbonyl-2- ethoxy-1, 2-dihydroquinoline (EEDQ) , pivaloyl chloride, l-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) , n-propane-phosphonic anhydride (PPA) , N,N-bis (2-oxo-3-oxazolidinyl) amido phosphoryl chloride (BOP-C1) , bromo-tris-pyrrolidinophosphonium hexafluorophosphate (PyBrop) , diphenylphosphoryl azide (DPPA) , Castro's reagent (BOP, PyBop) , 0-benzotriazolyl-N,N, N' , N' -tetramethyluronium salts (HBTU) , O-azabenzotriazolyl-N, N, N' , N' - tetramethyluronuim salts (TATU) , diethylphosphoryl cyanide (DEPCN) , 2, 5-diphenyl-2, 3-dihydro-3-oxo-4-hydroxythiophene dioxide (Steglich's reagent; HOTDO) , and 1,1'- carbonyldiimidazole (CDI) . The coupling reagents can be employed alone or in combination with additives such as N,N-dimethyl- 4-aminopyridine (DMAP) , N-hydroxy-benzotriazole (HOBt) , N-hydroxybenzotriazine (HOOBt) , N-hydroxysuccinimide (HOSu) or 2-hydroxypyridine . Although the use of protecting groups is generally not necessary in enzymatic peptide synthesis, reversible protection of reactive groups not involved in formation of the amide linkage is necessary for both reactants in chemical synthesis. Three conventional protective group techniques typically used for chemical peptide synthesis are: the benzyloxycarbonyl (Z) , the t-butoxycarbonyl (Boc) and the 9-fluorenylmethoxycarbonyl (Fmoc) techniques. Identified in each case is the protective group on the α-amino group of the chain-extending unit. A detailed review of amino-acid protective groups is given by Mύller, Methoden der organischen Chemie Vol. XV/l, pp 20-906, Thieme Verlag, Stuttgart (1974) . The units employed for assembling the peptide chain can be reacted in solution, in suspension or by a method similar to that described by Merrifield in J. Amer. Chem. Soc . 85 (1963) 2149. In one method, peptides are assembled sequentially or by fragment coupling using the Z, Boc or Fmoc protective group technique, with one of the reactants in the Merrifield technique being bonded to an insoluble polymeric support (also called resin hereinafter) . This typically entails assembling the peptide sequentially on the polymeric support using the Boc or Fmoc protective group technique, with the growing peptide chain covalently bonded at the C terminus to the insoluble resin particles. This procedure allows the removal of reagents and by- products by filtration, eliminating the need to recrystallize intermediates . The protected amino acids can be linked to any suitable polymer, which must be insoluble in the solvents used and have a stable physical form which permits filtration. The polymer must contain a functional group to which the first protected amino acid can be covalently attached. A wide variety of polymers are suitable for this purpose, for example, cellulose, polyvinyl alcohol, polymethacrylate, sulfonated polystyrene, chloromethylated styrene/divinylbenzene copolymer (Merrifield resin) , 4-methylbenzhydrylamine resin (MBHA-resin) , phenylacetamidomethyl resin (Pam-resin) , p-benzyloxy- benzyl-alcohol-resin, benzhydryl-amine-resin (BHA-resin) , 4- (hydroxymethyl) -benzoyl-oxymethyl-resin, the resin of Breipohl et al . { Tetrahedron Letters 28 (1987) 565; supplied by BACHEM) , 4- (2 , 4-dimethoxyphenylaminomethyl) phenoxy resin (supplied by Novabiochem) or o-chlorotrityl- resin (supplied by Biohellas) . Solvents suitable for peptide synthesis include any solvent which is inert under the reaction conditions, for example, water, N, N-dimethylformamide (DMF) , dimethyl sulfoxide (DMSO) , acetonitrile, dichloromethane (DCM) , 1,4-dioxane, tetrahydrofuran (THF) , N-methyl-2-pyrrolidone (NMP) and mixtures of these solvents. Peptide synthesis on the polymeric support can be carried out in a suitable inert organic solvent in which the amino acid derivatives and starting materials employed are soluble. Particularly useful solvents are, for example, DMF, DCM, NMP, acetonitrile, DMSO and mixtures thereof, due to their resin swelling properties. Following synthesis, the peptide is removed (commonly referred to as cleaved) from the polymeric support . The conditions under which this cleavage is accomplished are well known in the art of peptide synthesis and depend in part on the type of resin employed. The cleavage reactions most commonly used are acid- or palladium-catalyzed, the acid catalyzed cleavage being conducted in, for example, liquid anhydrous hydrogen fluoride, anhydrous trifluoromethanesulfonic acid, dilute or concentrated trifluoroacetic acid, and acetic acid/dichloromethane/ trifluoroethanol mixtures. The palladium-catalyzed cleavage can be carried out in THF or THF-DCM-mixtures in the presence of a weak base such as morpholine. Certain protecting groups are also cleaved off under these conditions. Partial deprotection of the peptide may also be necessary prior to certain derivatization reactions. For example, peptides dialkylated at the N-terminus can be prepared either by coupling the appropriate N,N-di- alkylamino acid to the peptide in solution or on the polymeric support or by reductive alkylation of the resin-bound peptide in DMF/l% acetic acid with NaCNBH 3 and the appropriate aldehyde or by hydrogenation of the peptide in solution in the presence of aldehyde or ketone and Pd/C. The various non-naturally occurring amino acids as well as the various non-amino acid moieties disclosed herein may be obtained from commercial sources or synthesized from commercially-available materials using methods known in the art. For example, amino acid building blocks with R 1 and R 2 moieties can be prepared according to E. Wuensch, Huben Weyl , Methoden der organischen Chemie Vol. XV/1, p. 306, Thieme Verlag, Stuttgart (1974) and literature cited therein. Peptides with gamma-or delta- lactam bridges can be prepared by incorporating the appropriate lactam-bridged dipeptide units (R. Freidinger, J " . Org. Chem. (1982) 104-109) into the peptide chain. Peptides with thiazole-, oxazol-, thiazolin- or oxazolin- containing dipeptide building blocks can be prepared by incorporating the appropriate dipeptidic units (P. Jouin et al . , Tetrahedron Letters (1992), pp. 2087-2810; P. Wipf et al . , Tetrahedon Letters (1992), pp. 907-910; W.R. Tully, J " . Med. Chem. (1991), p 2060-2065; U. Schmidt et al . , Synthesis (1987), pp 233-236) into the peptide chain. The following procedures are intended to illustrate methods useful for preparation of compounds of Forumla I. When applicable, amino acids are abbreviated using the known three letter codes . Other meanings used are : Me 2 Val=N,N-dimethylvaline, MeVal=N-methylvaline, TFA = trifluoroacetic acid, Ac = acetic acid, Bu = butyl, Et = ethyl, Me = methyl, Bzl = benzyl, Nal = 3 -naphthylalanine, Cha = 3-cyclohexylalanine, Npg = neopentyl glycine, Abu = 2-amino butyryl , Dab = 2 , 4-diaminobutyryl, iPr = isopropyl GENERAL SYNTHETIC PROCEDURES I . Compounds of Formula I of the present invention are either synthesized by classical solution synthesis using standard Z- and Boc-methodology as described above or by standard methods of solid-phase synthesis on a completely automatic model 431A synthesizer supplied by APPLIED BIOSYSTEMS . The apparatus uses different synthetic cycles for the Boc and Fmoc protective group techniques. In the case of solid phase synthesis, the N,N-dialkyl- penta- or hexapeptide acids are liberated from the solid support and further coupled with the corresponding C-terminal amines in solution. BOP-C1 and PyBrop were used as reagents for coupling of the amino acid following the N-methylamino acids. The reaction times were correspondingly increased. For reductive alkylation of the N-terminus, the peptide- resin was deprotected at the N terminus and then reacted with a 3 -fold molar excess of aldehyde or ketone in DMF/1% acetic acid with addition of 3 equivalents of NaCNBH 3 . After the reaction was complete (negative Kaiser test) the resin was washed several times with water, isopropanol, DMF and dichloromethane . In solution synthesis, the use of either Boc-protected amino acid NCAs (N-tert-butyloxycarbonyl-amino acid-N- carboxy-anhydrides) , Z-protected amino acid NCAs (N- benzyloxycarbonyl-amino acid-N-carboxy-anhydrides) , or the use of pivaloylchloride as condensing agent respectively is most advantageous for coupling of the amino acid following the N-methylamino acids. Reductive alkylation of the N terminus can, for example, be achieved by reaction of the N-terminally deprotected peptides or amino acids with the corresponding aldehydes or ketones using NaCNBH 3 or hydrogen, Pd/C. a) Synthetic cycle for the Boc protective group technique: 1. 30% trifluoroacetic acid in DCM 1 3 min 2. 50% trifluoroacetic acid in DCM l l min 3. DCM washing 4. 5% diisopropylethylamine in DCM 5 x 1 min 5. 5% diisopropylethylamine in NMP l l min 6. NMP washing 5 x 1 min 7. Addition of preactivated protected amino acid (DCC and 1 equivalent of HOBt in NMP/DCM) ; Peptide coupling (1st part) 1 x 30 min 8. Addition of DMSO to the reaction mixture until it contains 20% DMSO by volume; Peptide coupling (2nd part) 1 x 16 min 9. Addition of 3.8 equivalents of diisopropylethylamine to the reaction mixture ; Peptide coupling (3rd part) 1 x 7 min 10. DCM washing 3 x 1 min 11. If conversion is incomplete, repetition of coupling (back to 6) 12. 10% acetic anydride , 5% diisopropylethylamine in DCM 1 x 2 min 13. 10% acetic anhydride in DCM 1 x 4 min 14. DCM washing 4 x 1 min 15. Back to 1. BOP-Cl and PyBrop were used as reagents for coupling of the amino acid following N-methylamino acids. The reaction times were correspondingly increased. In solution synthesis, the use of either Boc-protected amino acid NCAs (N-tert-butyloxycarbonyl-amino acid-N-carboxy-anhydrides) or Z-protected amino acids NCAs respectively is most advantageous for this type of coupling. b) Synthetic cycle for the Fmoc protective group technique : 1. DMF washing l x l min 2. 20% piperidine in DMF 1 x 4 min 3. 20% piperidine in DMF 1 x 16 min 4. DMF washing 5 x 1 min 5. Addition of the preactivated protected amino acid (activation by 1 equivalent of TBTU and 5 equivalents of DIPEA in DMF) ; Peptide coupling 1 x 61 min 6. DMF washing 3 x 1 min 7. If conversion is incomplete, repetition of coupling (back to 5) 8. 10% acetic anhydride in DMF 1 x 8 min 9. DMF washing 3 x 1 min 10. Back to 2. BOP-Cl and PyBrop were used as reagents for coupling on the amino acid following the N-methylamino acids. The reaction times were correspondingly increased. II. Reductive Alkylation of the N-terminus The peptide-resin prepared in la or lb above was deprotected at the N-terminus (steps 2-4 in lb or 1-6 in la) and then reacted with a 3 -fold molar excess of aldehyde or ketone in DMF/1% acetic acid with addition of 3 equivalents of NaCNBH 3 . After reaction was complete (negative Kaiser test) the resin was washed several times with water, isopropanol, DMF and dichloromethane . III. Workup of the peptide-resins obtained as in la and II The peptide-resin was dried under reduced pressure and transferred into a reaction vessel of a TEFLON HF apparatus (supplied by PENINSULA) . Addition of a scavenger, for example, anisole (lml/g of resin), and in the case of tryptophan-containing peptides of a thiol to remove the indolic for yl group, for example, ethanedithiol (0.5 ml/g of resin), was followed by condensing in hydrogen fluoride (10 ml/g of resin) while cooling with liquid N 2 . The mixture was allowed to warm to 0°C and stirred at this temperature for 45 minutes. The hydrogen fluoride was then stripped off under reduced pressure, and the residue was washed with ethyl acetate in order to remove remaining scavenger. The peptide was extracted with 30% acetic acid and filtered, and the filtrate was lyophilized. IV. Work-up of the peptide-resins obtained as in lb and II The peptide-resin was dried under reduced pressure and then subjected to one of the following cleavage procedures, depending on the amino acid composition (Wade, Tregear, Howard Florey Fmoc Workshop Manual, Melbourne 1985). Cleavage conditions: TFA Scavenger Reaction time 1. 95% 5% water 1.5 h 2. 95% 5% ethanethiol/ anisole (1:3) 1,5 h The suspension of the peptide-resin in the suitable TFA mixture was stirred at room temperature for the stated time and then the resin was filtered off and washed with TFA and DCM. The filtrate and the washings were concentrated, and the peptide was precipitated by addition of the diethyl ether. After cooling in an ice bath, the precipitate was filtered off, taken up in 30% acetic acid and lyophilized. V. When an o-chlorotrityl-resin (supplied by Biohellas) is used, the suspension of the peptide-resin in an acetic acid/ trifluoroethanol/ dichloromethane mixture (1:1:3) is stirred at room temperature for 1 h. The resin is then filtered off with suction and thoroughly washed with the cleavage solution. The combined filtrates are concentrated in vacuo and treated with water. The precipitated solid is removed by filtration or centrifugation, washed with diethyl ether and dried under reduced pressure. VI . Purification and characterization of the peptides Purification was carried out by gel chromatography (SEPHADEX G-10, G-15/10% HOAc, SEPHADEX LH20/MeOH) medium pressure chromatography (stationary phase: HD-SIL C-18, 20-45 micron, 100 Angstrom; mobile phase: gradient with A=0.1% TFA/MeOH, B=0.1% TFA/water) or preparative HPLC (stationary phase: water Delta-Pak C-18, 15 micron, 100 Angstrom; mobile phase: gradient with A= 0.1% TFA/MeOH, B= 0.1% TFA/water) . The purity of the resulting products was determined by analytical HPLC (stationary phase: 100 2.1 mm VYDAC C-18, 51, 300 Angstrom; mobile phase: acetonitrile-water gradient, buffered with 0.1% TFA, 40°C) . Characterization was by amino acid analysis and fast atom bombardment mass spectroscopy. SPECIFIC SYNTHETIC PROCEDURES EXAMPLE 1A: N, N-dimethyl-Val-Val-N-methyl-Val-Pro-Pro- Val-Phe-NH 2 1.98 g of Fmoc-RINK-resin (substitution 0.46 mmol/g) , corresponding to a batch size of 0.84 mmol, were reacted as in lb above with 1.26 mmol each of Fmoc-Phe-OH Fmoc-Val-OH Fmoc-Pro-OH Fmoc-Pro-OH Fmoc-N-methyl-Val-OH Fmoc-Val-OH Fmoc-Val-OH The amino acid following the N-methyl amino acid was coupled on with PyBrop as coupling reagent. After the iterative synthetic cycles were completed, the peptide- resin underwent N-terminal deprotection (steps 2-4 in lb) , and was further reacted with aqueous formaldehyde solution as in II and then dried under reduced pressure. The resulting resin was subjected to TFA cleavage as in IV. The crude product (590 mg) was purified by gel filtration (SEPHADEX-LH-20) . The yield was 295 mg. EXAMPLE 1A: Example 1 can also be prepared via classical solution phase methodology. The synthesis of N,N-dimethyl-Val-Val- SUBSTΓΓUTE SHEET (RULE 26) N-methyl-Val-Pro-Pro-Val-Phe-NH 2 and its associated intermediates is described in the following paragraph. a) Z-MeVal-Pro-OMe 66.25 g (250 mmol) of Z-MeVal-OH were dissolved in 250 ml of dry dichloromethane. After addition of 36.41 ml (262.5 mmol) of triethylamine, the reaction mixture was cooled to -25°C and 32.37 ml (262.5 mmol) pivaloyl chloride were added. After stirring for 2.5 hours, 41.89g (250 mmol) of H-Pro-OMe-HCl in 250 ml of dichloromethane, neutralized with 36.41 ml (262.5 mmol) triethylamine at 0°C, were added to the reaction mixture. Stirring was continued for 2h at -25°C and overnight at room temperature. The reaction mixture was diluted with dichloromethane and thoroughly washed with saturated aqueous NaHC0 3 solution (3X) , water (IX), 5% citric acid (3X) and saturated NaCl solution. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness . The residue (91.24 g) was stirred with petroleum ether overnight and filtered. 62.3 g of product were obtained. b) H-MeVal-Pro-OMe 48.9 g (130 mmol) Z-MeVal-Pro-OMe were dissolved in 490 ml of methanol . After addition of 10.9 ml (130 mmol) concentrated hydrochloric acid and 2.43 g of 10% palladium/charcoal, the reaction mixture was hydrogenated. Filtration and evaporation to dryness yielded 36.43 g of product. c) Z-Val-MeVal-Pro-OMe 18.1 g (65 mmol) of H-MeVal-Pro-OMe, 21.6 g (78 mmol) Z-Val-N-carboxyanhydride and 22.8 ml (130 mmol) diisopropylethylamine were stirred in 110 ml of DMF at 40°C for 2 days. After evaporation of DMF, dichloromethane was added and the organic phase washed with saturated aqueous NaHC0 3 solution (3X) , water (IX) 5% citric acid (3X) and saturated NaCl solution. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness. The product (29.3 g) was obtained as a viscous oil. d) H-Val-MeVal-Pro-OMe 29.3 g (61.6 mmol) of Z-Val-MeVal-Pro-OMe were dissolved in 230 ml of methanol . After addition of 1.15 g of 10% palladium/charcoal, the reaction mixture was hydrogenated. Filtration and evaporation to dryness yielded 21.96 g of product. e) Z-Val-Val-MeVal-Pro-OMe 15.29 g (61 mmol) of Z-Val-OH and 21.96 g (61 mmol) of H-Val-MeVal-Pro-OMe were dissolved in 610 ml of dichloromethane and cooled to 0°C. After addition of 8.16 mol(73.2 mmol) of N-methylmorpholine, 2.77 g (20.3 mmol) of HOBt and 11.74g (61 mmol) of EDCI , the reaction mixture was stirred overnight at room temperature, diluted with dichloromethane and thoroughly washed with saturated aqueous NaHC0 3 solution (3X) , water (IX) , 5% citric acid (3X) and saturated NaCl solution. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness to yield 31.96 g of the product. f) Z-Val-Val-MeVal-Pro-OH 31.96 g (57 mmol) of Z-Val-Val-MeVal-Pro-OMe were dissolved in 250 ml of methanol . 102.6 ml of a IN LiOH solution was added and the mixture stirred overnight at room temperature. After addition of 500 ml of water, the aqueous phase was washed three times with ethyl acetate. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness yielding 30.62 g of the desired product as a white solid. g) Z-Val-Val-MeVal-Pro-Pro-Val-Phe-NH 2 25 g (43.3 mmol) of Z-Val-Val-MeVal-Pro-OH and 15.59 g (43.3 mmol) of H-Pro-Val-Phe-NH 2 were suspended in 430 ml of dry dichloromethane. After cooling to 0°C, 5.81 ml (52 mmol) N-methylmorpholine, 1.97 g (15 mmol) of HOBt and 8.33 g (43.3 mmol) of EDCI were added and the reaction mixture stirred overnight at room temperature. The solvents were evaporated, the residue dissolved in 640 ml of dichloromethane and thoroughly washed with saturated aqueous NaHC0 3 solution (4X) , water (IX) , 5% citric acid (3X) and saturated NaCl solution. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness to yield 33.04 g of the product. The crude product was chromatographed on a silica gel column with 20% MeOH/hexane. 18.32 g of the desired product were obtained. h) N,N-dimethyl-Val-Val-MeVal-Pro-Pro-Val-Phe-NH 2 18.32 g of Z-Val-Val-MeVal-Pro-Pro-Val-Phe-NH 2 were dissolved in 80 ml of methanol. 0.4 g of 10% palladium/carbon were added under nitrogen atmosphere and the reaction mixture hydrogenated at room temperature for 4 hours. After addition of 6.22 ml (81.24 mmol) of a 37% aqueous formaldehyde solution, hydrogenation was continued for 5 hours. Filtration and evaporation of the solvent gave rise to 15.6 g of crude product. Further purification was achieved by dissolving the peptide in water, adjusting the pH to 2 and extracting the aqueous phase three times with ethyl acetate. The aqueous phase was then adjusted to pH 8-9 and extracted four times with ethyl acetate. The organic phase was washed with water and dried over sodium sulfate, filtered and evaporated to yield 11.3 g of purified product as a white powder. The compound was characterized by fast atom bombardment mass spectrometry ( [M+H] + = 797) . EXAMPLE 2A: N, -dimethyl-Val-Val-NMe-Val-Pro- { 1- [thiazol- (2) -yl] -2 -phenyl } -ethylamide 4.11 g of Fmoc-Pro-p-alkoxybenzyl-alcohol-resin (substitution 0.73 mmol/g) , corresponding to a batch size of 3 mmol, were reacted as in lb with 4.5 mmol each of Fmoc-N-MeVal-OH Fmoc-Val-OH Fmoc-Val-OH The amino acid following the N-methylamino acid was in this case reacted with double coupling using PyBrop or Bop-Cl with increased reaction times. After the synthesis was complete, the peptide-resin underwent N-terminal deprotection (Steps 2-4 in lb) , and was further reacted with aqueous formaldehyde solution as in II and then dried under reduced pressure. The resin obtained in this way was subjected to TFA cleavage as in IV. The crude product (750 mg) was employed directly for the next coupling. 100 mg of this compound were reacted with 45 mg of (S) -2- [l-amino-2- SUBSTΓΓUTE SHEET (RULE 26) phenylethyl] thiazole and 230 mg of PyBop with the addition of 192 microliters of DIPEA in DMF at room temperature for 2 days. The reaction mixture was purified by gel chromatography (SEPHADEX LH-20, methanol) and the product fractions were combined. 83 mg of product were obtained. EXAMPLE IB Me 2 Val-Val-MeVal-Pro-Pro-NHCH(CH 3 ) 2 a) Z-MeVal-Pro-OMe 66.25 g (250 mmol) Z-MeVal-OH were dissolved in 250 ml dry dichloromethane. After addition of 36.41 ml (262.5 mmol) triethylamine, the reaction mixture was cooled to -25°C and 32.27 ml (262.5 mmol) pivaloyl chloride were added. After stirring for 2.5 h, 41.89 g (250 mmol) H-Pro-OMe x HC1 in 250 ml dichloromethane, neutralized with 36.41 ml (262.5 mmol) triethylamine at 0°C, were added to the reaction mixture. Stirring continued for 2 h at -25°C and overnight at room temperature. The reaction mixture was diluted with dichloromethane and thoroughly washed with saturated aqueous NaHC0 3 solution (3x) , water (lx) , 5% citric acid (3x) and saturated NaCl solution. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness. The residue (91.24 g) was stirred with petroleum ether overnight and filtered. 62.3 g of product were obtained. b) H-MeVal-Pro-OMe 48.9 g (130 mmol) Z-MeVal-Pro-OMe were dissolved in 490 ml methanol. After addition of 10.9 ml (130 mmol) concentrated hydrochloric acid and 2.43 g 10 % Palladium/charcoal, the reaction mixture was hydrogenated. Filtration and evaporation to dryness yielded 36.43 g of the product. c ) Z -Val -MeVal - Pro-OMe 18.1 g (65 mmol) H-MeVal-Pro-OMe, 21.6 g (78 mmol) Z- Val-N-carboxyanhydride and 22.8 ml (130 mmol) diisopropylethylamine were stirred in 110 ml DMF at 40°C for 2 d. After evaporation of DMF, dichloromethane was added and the organic phase washed with saturated aqueous NaHC0 3 solution (3x) , water (lx) , 5% citric acid (3x) and saturated NaCl solution. The organic phase was dried over sodium sulfate and evaporated to dryness. The product (29.3 g) was obtained as a viscous oil. d) H-Val-MeVal-Pro-OMe 29.3 g (61.6 mmol) of Z-Val-MeVal-Pro-OMe were dissolved in 230 ml methanol. After addition of 1.15 g 10% Palladium/charcoal, the reaction mixture was hydrogenated. Filtration and evaporation to dryness yielded 21.96 g of the product. e) Z-Val-Val-MeVal-Pro-OMe 15.29 g (61 mmol) Z-Val-OH and 21.96 g (61 mmol) H- Val-MeVal-Pro-OMe were dissolved in 610 ml dichloromethane and cooled to 0°C. After addition of 8.16 ml (73.2 mmol) N-Methylmoropholine, 2.77 g (20.3 mmol) HOBt and 11.74 g (61 mmol) EDCI, the reaction mixture was stirred overnight at room temperature, diluted with dichloromethane and thoroughly washed with saturated aqueous NaHC0 3 solution (3x) , water (lx) , 5% citric acid (3x) and saturated NaCl solution. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness to yield 31.96 g of the product . f ) Z-Val -Val -MeVal-Pro-OH 31.96 g (57 mmol) Z-Val-Val-MeVal-Pro-OMe were dissolved in 250 ml methanol. 102.6 ml of a 1 N LiOH solution was added and the mixture stirred overnight at room temperature. After addition of 500 ml water, the aqueous phase was washed three times with ethyl acetate, adjusted to pH 2 at 0°C and extracted three times with ethyl acetate. The organic phase was dried over sodium sulfate, filtered and evaporated to dryness yielding 30.62 g of the desired product as a white solid. g) Z-Val-Val-MeVal-Pro-Pro-NHCH (CH 3 ) 2 2 g (3.35 mmol) Z-Val-Val-MeVal-Pro-OH and 0.664 g (3.35 mmol) H-Pro-NHCH (CH 3 ) 2 were dissolved in 34 ml of dry dichloromethane. After cooling to 0°C, 1.35 ml (12.1 mmol) N-methylmorpholine, 0.114 g (0.84 mmol) HOBt and 0.645 g (3.35 mmol) EDCI were added and the reaction mixture stirred overnight at room temperature. 80 ml dichloromethane were added and the organic phase thoroughly washed with saturated aqueous NaHC0 3 solution (3x) , water (lx) , 5% citric acid (3x) and saturated NaCl solution (lx) . The organic phase was dried over sodium sulfate, filtered and evaporated to dryness to yield 1.96 g of the product which was used in the next reaction without further purification. h) Me 2 Val-Val-MeVal-Pro-Pro-NHCH (CH 3 ) 2 1.96 g Z-Val-Val-MeVal-Pro-Pro-NHCH(CH 3 ) 2 were dissolved in 11 ml methanol. 0.054 g 10% Pd/C were added under nitrogen atmosphere and the reaction mixture hydrogenated at room temperature for 4 h. After addition of 0.86 ml (11.24 mmol) of a 37% aqueous formaldehyde solution and 0.281 g 10% Pd/C, hydrogenation was continued for 5 h. Filtration and evaporation of the solvent gave rise to 2.77 g of crude product. Further purification was achieved by dissolving the peptide in water, adjusting the pH to 2 and extracting the aqueous phase three times with ethyl acetate. The aqueous phase was then adjusted to pH 8-9 and extracted four times with dichloromethane. The organic phase was dried over sodium sulfate, filtered and evaporated to yield 1.37 g of purified product as a white foam. The compound was further purified using medium pressure liquid chromatography (10-50% A in 10 min.; 50-90% A in 320 min.). Fractions containing the product were combined, lyophilized, redissolved in water and the pH adjusted to 9 with 1 N LiOH. After extraction with dichloromethane, the organic phase was dried over sodium sulfate, filtered and evaporated to dryness. Lyophilization led to 500 mg of pure product, which was characterized by fast atom bombardment mass spectrometry ( [M+H] + = 593). EXAMPLE 2B Me 2 Val-Val-MeVal-Pro-Pro-NHC(CH 3 ) 3 a) Z-Val-Val-MeVal-Pro-Pro-NHC (CH 3 2)1 3 2 g (3.35 mmol) Z-Val-Val-MeVal-Pro-OH and 0.692 g (3.35 mmol) H-Pro-NHC (CH 3 ) 3 were dissolved in 34 ml of dry dichloromethane. After cooling to 0°C, 1.35 ml (12.1 mmol) N-methylmorpholine, 0.114 g (0.84 mmol) HOBt and 0.645 g (3.35 mmol) EDCI were added and the reaction mixture stirred overnight at room temperature. 80 ml dichloromethane were added and the organic phase thoroughly washed with saturated aqueous NaHC0 3 solution (3x) , water (lx) , 5% citric acid (3x) and saturated NaCl solution (lx) . The organic phase was dried over sodium sulfate, filtered and evaporated to dryness to yield 1.8 g of the product which was used in the next reaction without further purification. b) Me 2 Val-Val-MeVal-Pro-Pro-NHC(CH 3 ) 3 1.8 g Z-Val-Val-MeVal-Pro-Pro-NHC(CH 3 ) 3 were dissolved in 10 ml methanol. 0.045 g 10% Pd/C were added under nitrogen atmosphere and the reaction mixture hydrogenated at room temperature for 4 h. After addition of 0.86 ml (11.24 mmol) of a 37% aqueous formaldehyde solution and 0.252 g 10% Pd/C, hydrogenation was continued for 5 h. Filtration and evaporation of the solvent gave rise to 1.82 g of crude product . The compound was further purified using medium pressure liquid chromatography (10-50% A in 10 min.; 50-90% A in 320 min.) . Fractions containing the product were combined, lyophilized, redissolved in water and the pH adjusted to 9 with 1 N LiOH. After extraction with dichloromethane, the organic phase was dried over sodium sulfate and evaporated to dryness. Lyophilization led to 547 mg of pure product, which was characterized by fast atom bombardment mass spectrometry ( [M+H] + = 607). EVALUATION OF BIOLOGICAL ACTIVITY In vivo Methodology The Dolastatin-15 derivative of Formula I, designated compound ii in Figure 1, was tested using a standard animal model for rheumatoid arthritis known as Collagen induced arthritis (CIA) (See, e.g., Banerjee, et al . , The Journal of Immunology 142 : 2237-2243 (1989) ) . CIA is a useful animal model of rheumatoid arthritis that serves as an in vivo system for the exploration of inflammatory synovitis etiologies and for the investigation of potentially new therapeutic interventions . Other suitable models can also be used in this invention. For example, Adjuvant Induced Arthritis in Rats (see, e.g., Ward, et al . , Arthritis Rheum. (1962) 5:557-564) . Collagen induced arthritis in mice in induced by intradermal injection of chick collagen type II emulsified in complete Freund's adjuvant, with the onset of symptoms typically occurring on or around day 26 post immunization. In general, any dosing regimen which appears to provide an acceptable level of suppression of rheumatoid arthritis is suitable . Any acceptable method of drug administration can be determined using techniques well known to those of skill in the art. In addition, the Dolastatin-15 derivatives of Formula I can be administered in combination with other drugs known to be useful in the treatment of rheumatoid arthritis, as described earlier. EXAMPLE 3 : COLLAGEN INDUCED ARTHRITIS - PROPHYLACTIC MODEL DBA-1 mice, which is a strain of mouse susceptible to collagen induced arthritis, were used in all experiments (See e.g., The FASEB, 2 : 2950 (1988)). Mice were immunized intradermally on day 0 with 100 μg of chick collagen type II in complete Freud's adjuvant. Three treatment groups were evaluated and consisted of saline treated animals (control) , dexamethasone treated animals (standard therapy), and compound ii treated animals. Treatment was commenced for all groups on day 26 post immunization just prior to the onset of symptoms and was ended on day 35 post immunization. Dexamethasone was injected intraperitoneally at a dose of 5 mg/kg/day, compound ii was given orally, by gavage, at a dose of 50 mg/kg/day SUBSTΓΠΠΈ SHEET (RULE 25) using saline as the vehicle and saline was administered orally once a day as a control . MEAN ARTHRITIC SCORE: The degree of arthritis severity was recorded by daily observation of each paw. An integer scale of 0-5 was used to quantify the level of erythema, swelling, deformity and joint stiffness in each paw with 0=normal and 5=maximum. The sum of all four paws represents the mean arthritic score, with a score of 20 being the maximum. The results are depicted graphically in Figure 2. The results show that none of the animals treated with compound ii had signs of rheumatoid arthritis up to 6 days after the end of treatment . The dexamethasone treated animals, however, exhibited signs of rheumatoid arthritis immediately following the end of treatment . EXAMPLE 4 : COLLAGEN INDUCED ARTHRITIS - THERAPEUTIC MODEL DBA-1 mice were used in all experiments. Mice were immunized intradermally on day 0 with 100 μg of chick collagen type II. Symptom onset occurred around day 35 post immunization Three treatment groups were evaluated and consisted of vehicle treated animals (control) , dexamethasone treated animals (standard therapy), and compound ii treated animals. Treatment was commenced for all groups on day 48 post immunization, when the arthritic score of all animals had reached 3-4. The mean arthitic scores of mice in the three groups were equivalent at the start of treatment . Animals were treated for 21 days. Dexamethasone was injected intraperitoneally at a dose of 5 mg/kg/day, compound ii was given orally by gavage at a dose of 50 mg/kg/day using saline as a vehicle and vehicle alone was administered by gavage (0.25 ml) as control. MEAN ARTHRITIC SCORE: The degree of arthritis severity was recorded by daily scoring of each paw. An integer scale of 0-5 was used to quantify the level of erythema, swelling, deformity and joint stiffness with 0=normal and 5=maximum. The sum of all four paws represents the mean arthritic score with a score of 20 being the maximum. The results are depicted graphically in Figure 3. The results show that animals treated with compound ii showed a significant decrease in mean arthritic score as compared to control (P Value less than 0.01-0.05, as determined by the Mann-Whitney Test) . HISTOPATHOLOGICAL RESULTS : Five mice from each treatment group were necropsied on day 71 post immunization and histopathology was performed on the joints from all four paws from each mouse. Both synovial inflammation and cartilage damage of affected joints were graded on a scale from 0-3. Results are shown in Figure 4. Treatment with compound ii and dexamethasone significantly suppressed synovitis and cartilage involvement as compared to the vehicle treated animals. EXAMPLE 5: ADJUVANT INDUCED ARTHRITIS RAT- ROPHYLACTIC MODEL Male Lewis rats were immunized intradermally on day 0 with 1.2 mg of heat-killed M. Tuberculosis in incomplete Freund's adjuvant. The treatment groups (10 animals/group) consisted of saline treated animals (control) , Methotrexate treated animals (standard therapy) 1 mg/kg/day and three groups treated with compound ii at 10, 5, and 2.5 mg/kg/day, respectively. Treatment started on the day of immunization (day 0) and continued once every other day for a total of 12 administrations. All the treatments were given orally by gavage. The animals were evaluated on days 12, 16, 19 and 23 by determining the mean arthritic scores in a manner similar to Examples 3 and 4. On day 23 the experiment was terminated, and the results showed that compound ii prevented the onset of arthritic signs (inflamed paws and limbs) in a dose dependent manner. That is, none of the animals treated with 10 mg/kg of compound ii showed signs of disease (0/10) , while 2/10 animals and 7/10 animals showed signs of disease in the 5 mg and 2.5 mg doses, respectively. In the control group, 7/10 animals showed signs of disease. Methotrexate prevented arthritis as expected. The following compounds were prepared and can be prepared according to the Examples : 3. Xaa Val Xab Pro Xac 4. Xaa Val Xab Pro Xad 5. Xaa Val Xab Pro Xae 6. Xaa Val Xab Pro Xaf 7. Xaa Val Xab Pro Xag 8. Xaa Val Xab Pro Xah 9. Xaa Val Xab Pro Xai 10. Xaa Val Xab Pro Xak 11. Xaa Val Xab Pro Xal 12. Xaa Val Xab Pro Xam 13. Xaa Val Xab Pro Xan 14. Xaa Val Xab Pro Xao 15. Xaa Val Xab Pro Xap 16. Xaa Val Xab Pro Xaq 17. Xaa Val Xab Pro Xar 18. Xaa Val Xab Pro Xas 19. Xaa Val Xab Pro Xat 20. Xaa Val Xab Pro Xau 21. Xaa Val Xab Pro Xav 22. Xaa Val Xab Pro Xaw 23. Xaa Val Xab Pro Xax 24. Xaa Val Xab Pro Xay 25. Xaa Val Xab Pro Xaz 26. Xaa Val Xab Pro Xba 27. Xaa Val Xab Pro Xbb 28. Xaa Val Xbc Pro Xay 29. Xaa Val Xab Pro Xbd 30. Xaa Val Xab Pro Xbe 31. Xaa Val Xab Pro Xbf 32. Xaa Val Xab Pro Xbg 33. Xaa Val Xab Pro Xbh 34. Xaa Val Xab Pro Xbi 00 H U a. I 00 I rH B β 0 ft Γ 1 m β > 5 X N rd XI U J (L> 4-1 -β -H rH ε β 0 ft tJ 1 ra X. XI XI XI XI XI XI XI X. XI XI XI XI XI X} XI U υ υ υ U u rd u υ υ υ υ U U U υ υ υ X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 0 0 0 0 0 0 0 0 o 0 O O 0 O 0 0 0 0 0 0 O 0 O o 0 0 0 0 o 0 o O 0 o 0 ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft X. XI XI -Q XI XI XI XI XI X. XI XI XI XI X. X. X. XI X. XI X. XI X. XI XI X. XI X. X. X. XI X) rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH rH 4-. rH rH rH rH rH rH rH rH rH rH rH rH rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd ) rd rd rd rd rd rd rd rd rd rd rd rd > > > > > > > > > > > > > > > > > > > > > > X > > > > > > > > > > > > rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd rd X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X t> m 3 o σ. o H CN > in O co σi o H rn in σ. o H CN ro • Φ in VO r 00 σ. m ro m ■ φ ■ Φ ■ φ m in in in in in m in in in lO Ov 70. Xaa Val Xab Pro Xcu 71. Xcw Val Xab Pro Xcv 72. Xcx Val Xab Pro Xcv 73. Xaa Val Xab Pro Pro Xcy 74. Xaa Val Xab Pro Pro Xcz 75. Xaa Val Xda Pro Xcv 76. Xaa Xdb Xab Pro Xcv 77. Xdc Val Xab Pro Xcv 78. Xaa He Xab Pro Xcv 79. Xdd Val Xab Pro Xcv 80. Xde Val Xab Pro Xcv 81. Xaa Xdf Xab Pro Xcv 82. Xaa Val Xab Pro Xcg 83. Xaa Val Xab Pro Pro Xdg 84. Xaa Val Xab Pro Pro Xdh 85. Xaa Val Xab Pro Pro Xdi 86. Xaa Val Xab Pro Pro Xdk 87. Xaa Val Xdl Pro Xcv 88. Xde Val Xab Pro Xay 89. Xaa Val Xdl Pro Xay 90. Xaa Val Xab Pro Xdm 91. Xaa Val Xab Pro Xdn 92. Xaa Val Xab Pro Xdo 93. Xaa Val Xab Pro Xdp 94. Xaa Val Xab Pro Xdq 95. Xaa Val Xab Pro Pro Xdr 96. Xaa Val Xab Pro Xds 97. Xaa Val Xbc Pro Xcv 98. Xaa He Xab Pro Xay 99. Xcw Val Xab Pro Xay 100. Xaa Val Xbc Pro Xal 101. Xaa Val Xdl Pro Xal 102. Xaa Xdf Xab Pro Xal 103. Xaa He Xab Pro Xal 104. Xdd Val Xab Pro Xal 105. Xde Val Xab Pro Xal 106. Xcx Val Xab Pro Xcy 107. Xcw Val Xab Pro Xal 108. Xcx Val Xab Pro Xal 109. Xcw Val Xab Pro Xav 110. Xcx Val Xab Pro Xav 111. Xcw Val Xab Pro Xaw 112. Xcx Val Xab Pro Xaw 113. Xab Val Xab Pro Xay 114. Xab Val Xab Pro Xcv 115. Xab Val Xab Pro Xal 116. Xab Val Xab Pro Xam 117. Xab Val Xab Pro Xan 118. Xab Val Xab Pro Xao 119. Xab Val Xab Pro Xav 120. Xab Val Xab Pro Xaw 121. Xab Val Xab Pro Xat 122. Xab Val Xab Pro Xau 123. Xab Val Xab Pro Xbf 124. Xab Val Xab Pro Xbm 125. Xab Val Xab Pro Xbn 126. Xab Val Xab Pro Xbo 127. Xab Val Xab Pro Xch 128. Xaa Val Xab Pro Xdt 129. Xaa Val Xab Pro Xdu 130. Xaa Val Xab Pro Xdv 131. Xaa Val Xab Pro Xdw 132. Xaa Val Xab Pro Xdx 133. Xaa Val Xab Pro Xdy 134. Xaa Val Xab Pro Xdz 135. Xaa Val Xab Pro Xea 136. Xaa Val Xab Pro Xeb 137. Xaa Val Xab Pro Xec 138. Xaa Val Xab Pro Xed 139. Xaa Val Xab Pro Xef 140. Xaa Val Xab Pro Xeg 141. Xaa Val Xab Pro Xeh 142. Xaa Val Xab Pro Xei 143. Xaa Val Xab Pro Xek 144. Xaa Val Xab Pro Xel 145. Xaa Val Xab Pro Xem 146. Xaa Val Xab Pro Xen 147. Xaa Val Xab Pro Xeo 148. Xaa Val Xab Pro Xep 149. Xaa Val Xab Pro Xeq 150. Xaa Val Xab Pro Xer 151. Xaa Val Xab Pro Xcq 152. Xaa Val Xab Pro Pro Val Phe 153. Xaa Val Xab Pro Xet Val Phe NH, 154. Xaa Val Xer Pro Pro Val Phe NH 2 155. Xaa Val Xbc Pro Pro Val Phe NH, 156. Xaa He Xab Pro Pro Val Phe NH 2 157. Xaa Leu Xab Pro Pro Val Phe NH 2 158. Xde Val Xab Pro Pro Val Phe NH 2 159. Xdd Val Xab Pro Pro Val Phe NH 2 160. Xes Val Xab Pro Pro Val Phe NH 2 161. Xeu Val Xab Pro Pro Val Phe NH 2 162. Xaa Val Xab Pro Pro Phe Phe NH 2 163. Xaa Val Xab Pro Pro Val NH 2 163. Xaa Val Xab Pro Xev 165. Xaa Val Xab Pro Pro NH 2 166. Xaa Val Xab Pro Pro 167. Xaa Val Xab Pro Xew 168. Xaa Val Xab Xex 169. Xdd Val Xab Pro Pro NH 2 170. Xaa Xdf Xab Pro Pro NH 2 171. Xaa Val Xab Pro Xey 172. Xaa Val Xab Pro Xez 173. Xfa Val Xab Pro Pro Val Phe NH 2 174. Xaa Val Xab Pro Pro Xfb 175. Xaa Val Xab Pro Xfc 176. Xaa Val Xab Pro Xfd 177. Xaa Val Xab Pro Xfe 178. Xaa Val Xab Pro Xff 179. Xaa Val Xab Pro Xfg 180. Xaa Val Xab Pro Xfh 181. Xaa Val Xab Pro Xfi 182. Xaa Val Xab Pro Xfj 183. Xaa Val Xdl Pro Pro NH 2 184. Xaa Val Xfk Pro Pro NH 2 185. Xaa Val Xfl Pro Xfh 186. Xaa Val Xfk Pro Xfh 187. Xcx Val Xab Pro Xfh 188. Xaa Val Xab Pro Pro Xdf Phe NH 2 189. Xaa Val Xab Pro Pro Leu Phe NH 2 190. Xaa Val Xab Pro Pro He Phe NH 2 Examples for the MS-characterization of the synthesized novel compounds are listed below: EXAMPLE Fast atom bombardment MS analysis 3. 565 4. 579 5. 593 6. 607 7. 621 8. 635 11. 607 12. 607 13. 621 14. 649 15. 635 16. 635 17. 635 18 635 19. 621 20. 621 21. 635 22. 635 25. 633 26. 647 27. 661 31. 623 32. 671 33. 667 34. 681 35. 655 36. 655 37. 669 38. 621 39. 635 41. 649 42. 621 43. 633 44. 667 45. 607 46. 647 47. 668 48. 655 49. 669 50. 685 51. 629 52. 625 53. 721 55. 579 58. 623 61. 597 62. 621 63. 609 64. 625 65. 635 66. 591 67. 715 68. 685 69. 685 70. 591 71. 607 72. 621 74. 706 75. 579 76. 579 77. 579 78. 607 79. 607 80. 607 81. 607 82. 637 83. 692 84. 706 85. 706 86. 706 87. 607 90. 635 92. 659 93. 617 94. 636 95. 678 128. 671 131. 625 139. 625 151. 637 152. 798 153. 810 154. 812 155. 812 156. 812 157. 812 258. 812 159. 811 160. 825 161. 881 162. 845 163. 649 164. 737 165. 550 166. 551 167. 731 168. 550 169. 566 170. 566 171. 635 172. 704 173. 853 174. 740 175. 619 176. 845 177. 649 178. 691 179. 717 180. 641 181. 579 182. 595 183. 566 184. 566 185. 669 186. 656 187. 669 188. 811 189. 812 190. 812 The symbols used in the description of the compounds of Formula I have the following meanings : Xaa: N,N-Dimethylvaline Xab: N-Methylvaline 0 5 0 0 0 H 3 C -5Ϊ CH 3 N-Methyl-isoleucine Xbc: O O 0 H 3 C H 3 C 0 o 0 0 0 45 Proline adamantyl (1) amide Xcc: 0 0 0 5 XCO: 0 - SUBSTITUTE SHEET (RULE 25) Xcw: N-Methyl-N- ethyl -valine Xcx: N,N-Diethylvaline Xda: N-Methyl-2-aminobutyroyl Xdb: 2-aminobutyroyl Xde: N,N-Dimethyl-2-aminobutyroyl Xdd: N,N-Dimethyl-2-tert.butylglycine Xde: N,N-Dimethyl-isoleucine Xdf: 2-tert.butylglycine CH 3 CH 3 0 CH 3 CH 3 Xdk: H 3 C'" _ N/ ^NH^/CH 3 0 CH 3 Xdl : N -Methyl-2-tert.butylglycine - CH 3 45 45 10 Cl Xer N-Methylleucine Xes N-Acetyl-N-methylvaline Xet pipecolinic acid Xeu N,N-Dibutylvaline Xfa: N , N-dipropylvaline Xfk: N-Ethylvaline Xf 1: N-Methyl-3-tert-butylalanine EQUIVALENTS Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed in the scope of the following claims.

Claims: CLAIMS What is claimed is: 1. A method for the treatment of rheumatoid arthritis in a mammal, comprising administering to said mammal a therapeutically effective amount of a compound of Formula I : R 1 R 2 N-CHX-CO-A-B-D- (E) s - (F) t - (G) u -K (I) wherein: R 1 is alkyl, cycloalkyl, alkylsulfonyl, fluoroalkyl, or aminosulfonyl; R 2 is hydrogen, alkyl, fluoroalkyl or cycloalkyl ; R x -N-R 2 together may be a pyrrolidino or piperidino residue; A is a valyl, isoleucyl, leucyl, allo- isoleucyl, 2 , 2-dimethylglycyl, 2- cyclopropylglycyl, 2-cyclopentylglycyl , 3- tert-butylalanyl, 2-tert-butylglycyl, 3- cyclohexylalanyl, 2-ethylglycyl, 2- cyclohexylglycyl, norleucyl or norvalyl residue; B is a N-alkyl -valyl, -norvalyl, -leucyl, - isoleucyl, -2-tert-butylglycyl, -3-tert- butylalanyl, -2-ethylglycyl, -2- cyclopropylglycyl , -2-cyclopentylglycyl , norleucyl or -2-cyclohexylglycyl residue; SUBSTITUTE SHEET (RULE 25) D is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl, 4 -fluoroprolyl, 3- methylprolyl, 4 -methylprolyl, 5 -methylprolyl, azetidine-2 -carbonyl, 3 , 3-dimethylprolyl, 4,4- difluoroprolyl , oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue; E is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl , 4 -fluoroprolyl, 3- methylprolyl, 4 -methyl prolyl, 5- methylprolyl, azetidine-2 -carbonyl, 3,3- dimethylprolyl , 4 , 4 -difluoroprolyl , oxazolidine-4 -carbonyl or thiazolidine-4- carbonyl residue; F and G are independently selected from the group consisting of prolyl, homoprolyl, hydroxyprolyl, thiazolidinyl -4 -carbonyl, 1- aminopentyl-1-carbonyl , valyl, 2-tert- butylglycyl, isoleucyl, leucyl, 3- cyclohexylalanyl , phenylalanyl, N- methylphenylalanyl, tetrahydrosioquinolyl-2- histidyl, 1-aminoindyl-l -carbonyl, 3- pyridylalanyl, 2-cyclohexylglycyl, norleucyl, norvalyl, neopentylglycyl, trytophanyl, glycyl, 2 , 2-dimethylglycyl, alanyl, β-alanyl and 3-naphthylalanyl residues; X is hydrogen, alkyl, cycloalkyl, -CH 2 - cyclohexyl or arylalkyl; s, t and u are independently 0 or 1; and K is hydroxy, alkoxy, phenoxy, benzyloxy or a substituted or unsubstituted amino moiety; and the salts thereof with physiologically tolerated acids . 2. A method of Claim 1 wherein said mammal is human. 3. A method of Claim 2 wherein for the compound of Formula I, K is a substituted amino moiety having the formula R 5 -N-R 6 wherein: R 5 is hydrogen, or hydroxy, or C λ . η alkoxy, or benzyloxy, or phenyloxy, or C l ^ 1 - linear or branched alkyl (which may be substituted by one or more fluoro atoms) , or C 1 . 12 linear or branched hydroxyalkyl, or C 3 _ 10 -cycloalkyl , or benzyl (which may be substituted by up to three substituents which may independently be CF 3 , nitro, Cj_ 7 alkylsulfonyl, C^ alkoxy, phenoxy, benzoxy, halogen, C^-alkyl, cyano, hydroxy, N(CH 3 ) 2 , COOMe, COOEt, COOiPr, or COONH 2 ) ; R 6 is hydrogen, Cι_ 12 linear or branched alkyl (which may be substituted by one or more fluoro atoms) , or C 2 linear or branched hydroxyalkyl, or C 3 _ 10 -cycloalkyl , or -(CH 2 ) V -C 3 _ 7 - cycloalkyl (v=0,l,2, or 3 ) , or norephedryl , or norpseudoephedryl , or quinolyl, or pyrazyl, or -CH 2 -benzimidazolyl, or (1) -adamantyl or (2) -adamantyl or -CH 2 - adamantyl, or alpha-methyl-benzyl , or alpha- dimethylbenzyl, or - (CH 2 ) v -phenyl (v=0,l,2, or 3; which may be substituted by up to two substituents which may independently be CF 3 , nitro, λ . η alkylsulfonyl, C^ alkoxy, phenoxy, benzoxy, halogen, C^-alkyl which may form a cyclic system, cyano, hydroxy, SUBSTITUTE SHEET (RULE 25) N(CH 3 ) 2 , COOMe, COOEt, COOiPr, or COONH 2 ) , or - (CH 2 ) m -naphthyl (m=0 or 1); or -(CH 2 ) W - benzhydryl (w=0,l, or 2); or biphenyl or picolyl or benzothiazolyl or benzoisothiazolyl or benzopyrazolyl or benzoxazolyl or - (CH 2 ) m -fluorenyl (m=0 or 1) ; or pyrimidyl or - (CH 2 ) m-indanyl (m=0 or 1) ; or - (CH 2 CH 2 0) y -CH 3 (y=0 , 1 , 2 , 3 , 4 , or 5), or - (CH 2 CH 2 0) y -CH 2 CH 3 (y=0,l,2,3,4, or 5), or NH- C 6 H 5 (which may be substituted by up to two substituents which may independently be CF 3 , nitro, C ! _ 7 alkylsulfonyl, C^,, alkoxy, halogen, Cι_ 4 alkyl which may form a cyclic system, cyano, hydroxy, COOMe, COOEt, COOiPr, or COONH 2 ) , or -NCH 3 -C 6 H 5 , or -NH-CH 2 -C 6 H 5 , or- NCH 3 -CH 2 -C 6 H 5 , or 5-membered heteroaryl (which may be substituted by up to two substituents which may independently be CF 3 , nitro, thiomethyl, thioethyl, C 3 _ 6 -cycloalkyl, -CH 2 - COOEt, C 3 _„ -alkylene group forming a bicyclic system with the heterocycle) , or phenyl, or - CHR 7 -5-membered heteroaryl (which may be substituted by up to two substituents which may independently be CF 3 , nitro, cyano, halogen, COOMe, COOEt, COOiPr, C0NH 2 , C x _ 4 - alkyl, C x _ 4 -alkoxy, phenyl, benzyl, naphthyl, or Ci. 7 - alkylsulfonyl [R 7 = hydrogen, linear or branched Cj_ 5 alkyl, benzyl; or R 7 and R 5 together form a group -(CH 2 ) 3 - or -(CH 2 ) 4 -). A method of Claim 3 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, isoleucyl or 2-tertbutylglycyl ; D is prolyl, 4- fluoroprolyl, thiazolidinyl-4 -carbonyl , or 3,4- dehydroprolyl ; E is prolyl, 4 -fluoroprolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or Ci-C,, alkoxy and R 6 is a C^C^ linear or branched alkyl group selected from the group of monovalent radicals consisting of: -C(CH 3 ) 3 , also referred to as tert-butyl; -C-CH.-CH 3 , also referred to as 1,1-dimethyl propyl; (CH 3 ) 2 -C (CH 2 -CH 3 ) ,, also referred to as 1-methyl-1-ethyl I CH 3 propyl -CH-C(CH 3 ) 3 , also referred to as (S) - or (R) -1-methyl- CH 3 2, 2 -dimethyl propyl; -CH-CH(CH 3 ) 2 , also referred to as (S) - or (R) -1-ethyl- C 2 H 5 2 -methyl propyl; -CH-CH (CH 3 ) 2 , also referred to as 1-isopropyl-2 -methyl CH(CH 3 ) 2 butyl; or -C (CH 3 ) 2 -CH(CH 3 ) 2 , also referred to as 1 , l-dimethyl-2- methylpropyl -CH(CH 3 ) 2 , also referred to as isopropyl -CH(CH 3 ) CH.CHj, also referred to as sec-butyl, (S) - or ( R)- -CH(CH 3 )CH(CH 3 ) 2 , also referred to as 1,2- dimethylpropyl . 5. A method of Claim 4 wherein said monovalent radical is -C(CH 3 ) 3 , also referred to as tert butyl. 6. A method of Claim 3 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, 1-isoleucyl or 2-tertbutylglycyl ; D is prolyl, 4- fluoroprolyl , thiazolidinyl -4 -carbonyl , or 3,4- dehydroprolyl; E is prolyl, 4 -fluoroprolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or Cj-C 4 alkoxy and R 6 is selected from the group of monovalent radicals consisting of: (CH 2 )v- phenyl (wherein v is 1), and , α-dimethylbenzyl . 7. A method of Claim 3 wherein for the compound of Formula I R" and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1 ; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, 1- isoleucyl or 2-tertbutylglycyl; D is prolyl, 4- fluoroprolyl, thiazolidinyl -4 -carbonyl, or 3,4- dehydroprolyl ; E is prolyl, -fluoroprolyl, thiazolidinyl -4 -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or Cj-C,, alkoxy and R 6 is a linear or branched hydroxyalkyl . 8. A method of Claim 7 wherein R 6 is 3 -hydroxy-1, 1- dimethylpropyl . 9. A method of Claim 3 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1 ; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, isoleucyl or 2-tertbutylglycyl; D is prolyl, 4- SUBSTTTUTE SHEET (RULE 26) fluoroprolyl, thiazolidinyl-4 -carbonyl, or 3,4- dehydroprolyl ; E is prolyl, 4 -fluoroprolyl, thiazolidinyl- -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or C^d alkoxy and R 6 is a C 3 . 10 cycloalkyl selected from the group consisting of: (1) -adamantyl, (2) -adamantyl, cyclobutyl, cyclopentyl, cyclohexyl, 1- methylcyclopentyl, 1-methylcyclohexyl and [3.3.0]octa- 1-yl. 10. A method of Claim 4 wherein for the compound of Formula I R 1 and R 2 are each methyl; X is isopropyl; s is 1; t and u are each 0; A is valyl; B is N- methylvalyl; D is prolyl; E is prolyl; R 5 is hydrogen and R 6 is tert-butyl. 11. A method of Claim 3 wherein for the compound of Formula I R 1 and R 2 are each methyl; X is isopropyl; s is 1; t and u are each 0; A is valyl; B is N- methylvalyl ; D is prolyl; E is prolyl; R 5 is benzyl and R 6 is hydrogen. 12. A method for the treatment of rheumatoid arthritis in a mammal, comprising administering to said mammal a pharmaceutical composition comprising: a) a therapeutically effective amount of a compound of Formula I: R 1 R 2 N-CHX-CO-A-B-D- (E) s - (F) t - (G) u -K (I) wherein: R 1 is alkyl, cycloalkyl, alkylsulfonyl, fluoroalkyl, or aminosulfonyl ; R 2 is hydrogen, alkyl, fluoroalkyl or cycloalkyl; R^N-R 2 together may be a pyrrolidino or piperidino residue; A is a valyl, isoleucyl, leucyl, allo-isoleucyl, 2 , 2-dimethylglycyl, 2-cyclopropylglycyl, 2- cyclopentylglycyl, 3 -tert-butylalanyl , 2-tertbutylglycyl, 3-cyclohexylalanyl, 2-ethylglycyl, 2-cyclohexylglycyl, norleucyl or norvalyl residue; B is a N-alkyl-valyl , -norvalyl, -leucyl, - isoleucyl, -2-tert-butylglycyl, -3-tert- butylalanyl, -2-ethylglycyl, -2- cyclopropylglycyl , -2 -cyclopentylglycyl , norleucyl or -2-cyclohexylglycyl residue; D is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl, 4-fluoroprolyl , 3 -methylprolyl, 4 -methylprolyl, 5 -methylprolyl , azetidine-2- carbonyl, 3 , 3-dimethylprolyl , 4,4- difluoroprolyl , oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue ; E is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl , 4-fluoroprolyl , 3 -methylprolyl, 4 -methyl prolyl, 5 -methylprolyl, azetidine-2- carbonyl, 3 , 3-dimethylprolyl , 4,4- difluoroprolyl, oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue; F and G are independently selected from the group consisting of prolyl, homoprolyl, hydroxyprolyl, thiazolidinyl-4 -carbonyl, 1- aminopentyl-1-carbonyl, valyl, 2-tert- SUBSTΓΓUTE SHEET (RULE 26) butylglycyl, isoleucyl, leucyl, 3- cyclohexylalanyl, phenylalanyl, N- methylphenylalanyl, tetrahydrosioquinolyl-2- histidyl, 1-aminoindyl-l-carbonyl , 3- pyridylalanyl, 2-cyclohexylglycyl , norleucyl, norvalyl, neopentylglycyl, trytophanyl, glycyl, 2 , 2-dimethylglycyl, alanyl, β-alanyl and 3-naphthylalanyl residues; X is hydrogen, alkyl, cycloalkyl, -CH 2 -cyclohexyl or arylalkyl; s, t and u are independently 0 or 1; and K is hydroxy, alkoxy, phenoxy, benzyloxy or a substituted or unsubstituted amino moiety; and the salts thereof with physiologically tolerated acids; and b) a therapeutically effective amount of a second antiarthritic drug selected from the group consisting of: a nonsteroidal antiinflammatory agent, an organic gold derivative, D- penicillamine, a 4-aminoquinoline, azathioprine, methotrexate, cyclosporin, an angiogenesis inhibitor, a monoclonal antibody to T cells, a_ monoclonal antibody to an adhesion molecule, a monoclonal antibody to a cytokine or growth factor, TNFR-IgG, IL-1 receptor antagonists and ICE inhibitors. 13. Use, for the manufacture of a medicament for the treatment of rheumatoid arthritis in a mammal, of a compound of Formula I: R 1 R 2 N- CHX- CO -A-B -D- ( E ) s - ( F ) c - ( G) u - K ( I ) wherein : R 1 is alkyl, cycloalkyl, alkylsulfonyl, fluoroalkyl, or aminosulfonyl ,- R 2 is hydrogen, alkyl, fluoroalkyl or cycloalkyl; R : -N-R 2 together may be a pyrrolidino or piperidino residue; A is a valyl, isoleucyl, leucyl, allo- isoleucyl, 2 , 2-dimethylglycyl , 2- cyclopropylglycyl , 2 -cyclopentylglycyl , 3- tert-butylalanyl, 2-tert-butylglycyl, 3- cyclohexylalanyl, 2-ethylglycyl, 2- cyclohexylglycyl , norleucyl or norvalyl residue; B is a N-alkyl-valyl, -norvalyl, -leucyl, - isoleucyl, -2-tert-butylglycyl, -3-tert- butylalanyl, -2-ethylglycyl, -2- cyclopropylglycyl , - 2 -cyclopentylglycyl , norleucyl or -2-cyclohexylglycyl residue; D is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl , 4-fluoroprolyl , 3- methylprolyl, 4 -methylprolyl , 5 -methylprolyl , azetidine-2 -carbonyl, 3 , 3 -dimethylprolyl , 4,4- difluoroprolyl, oxazolidine-4-carbonyl or thiazolidine-4 -carbonyl residue; E is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl, 4-fluoroprolyl , 3- methylprolyl , 4 -methyl prolyl, 5- methylprolyl, azetidine-2 -carbonyl , 3,3- SUBSTΓΓUTE SHEET (RULE 26) dimethylprolyl , 4 , 4 -difluoroprolyl , oxazolidine-4 -carbonyl or thiazolidine-4- carbonyl residue; F and G are independently selected from the group consisting of prolyl, homoprolyl, hydroxyprolyl, thiazolidinyl-4 -carbonyl , 1- aminopentyl-1 -carbonyl, valyl, 2-tertbutylglycyl, isoleucyl, leucyl, 3- cyclohexylalanyl, phenylalanyl , N- methylphenylalanyl, tetrahydrosioquinolyl-2- histidyl, 1-aminoindyl-l -carbonyl, 3- pyridylalanyl, 2-cyclohexylglycyl , norleucyl, norvalyl, neopentylglycyl, trytophanyl, glycyl , 2,2- dimethylglycyl , alanyl , β- alanyl and 3 -naphthylalanyl residues; X is hydrogen, alkyl, cycloalkyl, -CH 2 - cyclohexyl or arylalkyl; s, t and u are independently 0 or 1; and K is hydroxy, alkoxy, phenoxy, benzyloxy or a substituted or unsubstituted amino moiety; and the salts thereof with physiologically tolerated acids . 14. A process for the manufacture of a therapeutic composition for the treatment of rheumatoid arthritis in a mammal, characterized in the use, as an essential constituent of said composition, of a compound of Formula I as defined in Claim 13. 15. A method of Claim 13 or process of Claim 14 wherein said mammal is human. 16. The invention of any one of the Claims 13-15 wherein for the compound of Formula I, K is a substituted amino moiety having the formula R 5 -N-R 6 wherein: R 5 is hydrogen, or hydroxy, or C^-, alkoxy, or benzyloxy, or phenyloxy, or C 1-7 - linear or branched alkyl (which may be substituted by one or more fluoro atoms) , or C x _ 12 linear or branched hydroxyalkyl, or C 3 _ 10 -cycloalkyl, or benzyl (which may be substituted by up to three substituents which may independently be CF 3 , nitro, C x _ 7 alkylsulfonyl, C 1 . 4 alkoxy, phenoxy, benzoxy, halogen, cyano, hydroxy, N(CH 3 ) 2 , COOMe, COOEt, COOiPr, or COONH ) ; R 6 is hydrogen, C^, linear or branched alkyl (which may be substituted by one or more fluoro atoms) , or C^^ linear or branched hydroxyalkyl, or C 3 . 10 -cycloalkyl , or -(CH 2 ) V -C 3 _ 7 - cycloalkyl (v=0,l,2, or 3), or norephedryl, or norpseudoephedryl , or quinolyl, or pyrazyl, or -CH 2 -benzimidazolyl , or (1) -adamantyl or (2) -adamantyl or -CH 2 - adamantyl , or alpha-methyl -benzyl, or alpha- dimethylbenzyl, or - (CH 2 ) v -phenyl (v=0,l,2, or 3; which may be substituted by up to two substituents which may independently be CF 3 , nitro, C].., alkylsulfonyl, C^ alkoxy, phenoxy, benzoxy, halogen, C^-alkyl which may form a cyclic system, cyano, hydroxy, N(CH 3 ) 2 , COOMe, COOEt, COOiPr, or COONH,) , or - (CH 2 ) m -naphthyl (m=0 or 1); or -(CH 2 ) W - benzhydryl (w=0,l, or 2); or biphenyl or picolyl or benzothiazolyl or benzoisothiazolyl or benzopyrazolyl or benzoxazolyl or - (CH 2 ) m -fluorenyl (m=0 or 1) ; or pyrimidyl or - (CH 2 ) m-indanyl (m=0 or 1) ; or - (CH 2 CH 2 0) y -CH 3 (y=0, 1, 2, 3 , 4, or 5), or - (CH 2 CH 2 0) y -CH 2 CH 3 (y=0,l,2,3,4, or 5), or NH- C 6 H 5 (which may be substituted by up to two substituents which may independently be CF 3 , nitro, C 1 _ 1 alkylsulfonyl, C x _ 4 alkoxy, halogen, C^ alkyl which may form a cyclic system, cyano, hydroxy, COOMe, COOEt, COOiPr, or COONH 2 ) , or -NCH 3 -C 6 H 5 , or -NH-CH 2 -C 6 H 5 , or- NCH 3 -CH 2 -C 6 H 5 , or 5-membered heteroaryl (which may be substituted by up to two substituents which may independently be CF 3 , nitro, thiomethyl, thioethyl , C 3 _ 6 -cycloalkyl , -CH 2 - COOEt, C 3 _ 4 -alkylene group forming a bicyclic system with the heterocycle) , or phenyl, or - CHR 7 -5-membered heteroaryl (which may be substituted by up to two substituents which may independently be CF 3 , nitro, cyano, halogen, COOMe, COOEt, COOiPr, CONH 2 , C x _ 4 - alkyl, Cj.,, -alkoxy, phenyl, benzyl, naphthyl, or Ci- 7 - alkylsulfonyl [R 7 = hydrogen, linear or branched C λ . 5 alkyl, benzyl; or R 7 and R 5 together form a group -(CH 2 ) 3 - or -(CH 2 ) 4 -). 17. An invention of Claim 16 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl; X is - isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, isoleucyl or 2-tertbutylglycyl; D is prolyl, 4- fluoroprolyl , thiazolidinyl-4-carbonyl , or 3,4- dehydroprolyl; E is prolyl, 4-fluoroprolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R δ wherein R 5 is hydrogen or C 1 -C 4 alkoxy and R 6 is a C 1 -C 12 linear or branched alkyl group selected from the group of monovalent radicals consisting of: -C(CH 3 ) 3 , also referred to as tert-butyl; -C-CH.-CH 3 , also referred to as 1,1-dimethyl propyl; (CH 3 ) 2 ~ -C (CH 2 -CH 3 ) 2 , also referred to as 1-methyl-l-ethyl CH 3 propyl -CH-C(CH 3 ) 3 , also referred to as (S) - or (R) -1 -methyl- CH 3 2, 2 -dimethyl propyl; -CH-CH(CH 3 ) -,, also referred to as (S) - or (R) -1-ethyl- C 2 H 5 2 -methyl propyl; -CH-CH (CH 3 ) 2 , also referred to as 1- isopropyl-2 -methyl CH(CH 3 ) 2 ~ butyl; or -C(CH 3 ) 2 -CH(CH 3 ) 2 , also referred to as 1 , l-dimethyl-2- methylpropyl -CH(CH 3 ) 2 , also referred to as isopropyl -CH(CH 3 ) CH.CH 3 , also referred to as sec-butyl, (S) - or ( R ) - -CH(CH 3 ) CH(CH 3 ) 2 , also referred to as 1,2- dimethylpropyl . 18. An invention of Claim 17 wherein said monovalent radical is -C(CH 3 ) 3 , also referred to as tert butyl. 19. An invention of Claim 16 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, 1-isoleucyl or 2-tertbutylglycyl; D is prolyl, 4- fluoroprolyl, thiazolidinyl-4-carbonyl, or 3,4- dehydroprolyl ; E is prolyl, 4-fluoroprolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or C : -C 4 alkoxy and R 6 is selected from the group of monovalent radicals consisting of: (CH 2 )v- phenyl (wherein v is 1), and α, α-dimethylbenzyl . 20. An invention of Claim 16 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, 1-isoleucyl or 2-tertbutylglycyl; D is prolyl, 4- fluoroprolyl , thiazolidinyl-4-carbonyl , or 3,4- dehydroprolyl; E is prolyl, 4-fluoroprolyl, thiazolidinyl-4-carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or C x -C 4 alkoxy and R 6 is a C^C^ linear or branched hydroxyalkyl . 21. An invention of Claim 20 wherein R 6 is 3 -hydroxy- 1, 1- dimethylpropyl . 22. An invention of Claim 16 wherein for the compound of Formula I R 1 and R 2 are each methyl or ethyl ; X is isopropyl, sec-butyl or tert-butyl; s is 1; t and _u are each 0; A is valyl, 2-ethylglycyl, isoleucyl or 2- tert-butylglycyl; B is N-methylvalyl, 2-ethylglycyl, isoleucyl or 2-tertbutylglycyl; D is prolyl, 4- fluoroprolyl , thiazolidinyl-4-carbonyl, or 3,4- dehydroprolyl ,- E is prolyl, 4-fluoroprolyl, thiazolidinyl-4 -carbonyl, homoprolyl, 3,4- dehydroprolyl or hydroxyprolyl; and K is a substituted amino moiety having the formula R 5 -N-R 6 wherein R 5 is hydrogen or C 1 -C 4 alkoxy and R 6 is a C 3 _ 10 cycloalkyl selected from the group consisting of: (1) -adamantyl, (2) -adamantyl, cyclobutyl, cyclopentyl, cyclohexyl, 1- methylcyclopentyl, 1-methylcyclohexyl and [3.3.0]octa- 1-yl. 23. An invention of Claim 17 wherein for the compound of Formula I R 1 and R 2 are each methyl; X is isopropyl; s is 1; t and u are each 0; A is valyl; B is N- methylvalyl; D is prolyl; E is prolyl; R 5 is hydrogen and R 6 is tert-butyl. 24. An invention of Claim 17 wherein for the compound of Formula I R 1 and R 2 are each methyl; X is isopropyl; s is 1; t and u are each 0; A is valyl; B is N- methylvalyl; D is prolyl; E is prolyl; R 5 is benzyl and R 6 is hydrogen. 25. A pharmaceutical composition comprising: a) a therapeutically effective amount of a compound of Formula I : R 1 R 2 N-CHX-CO-A-B-D- (E) s - (F) t - (G) u -K (I) wherein: R 1 is alkyl, cycloalkyl, alkylsulfonyl, fluoroalkyl, or aminosulfonyl ; R 2 is hydrogen, alkyl, fluoroalkyl or cycloalkyl; R x -N-R 2 together may be a pyrrolidino or piperidino residue; A is a valyl, isoleucyl, leucyl, allo-isoleucyl, 2, 2-dimethylglycyl, 2-cyclopropylglycyl, 2- cyclopentylglycyl, 3-tert-butylalanyl , 2-tert- SUBSTΪTUTE SHEET (RULE 26) butylglycyl, 3-cyclohexylalanyl, 2- ethylglycyl, 2-cyclohexylglycyl, norleucyl or norvalyl residue; B is a N-alkyl -valyl, -norvalyl, -leucyl, - isoleucyl, -2-tert-butylglycyl, -3-tert- butylalanyl, -2-ethylglycyl, -2- cyclopropylglycyl , -2 -cyclopentylglycyl, norleucyl or -2-cyclohexylglycyl residue; D is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl, 4-fluoroprolyl, 3 -methylprolyl, 4 -methylprolyl, 5 -methylprolyl, azetidine-2- carbonyl, 3 , 3 -dimethylprolyl, 4,4- difluoroprolyl, oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue; E is a prolyl, homoprolyl, hydroxyprolyl, 3,4- dehydroprolyl , 4-fluoroprolyl, 3 -methylprolyl, 4 -methyl prolyl, 5 -methylprolyl , azetidine-2- carbonyl, 3 , 3 -dimethylprolyl , 4,4- difluoroprolyl, oxazolidine-4 -carbonyl or thiazolidine-4 -carbonyl residue; F and G are independently selected from the group consisting of prolyl, homoprolyl, hydroxyprolyl, thiazolidinyl-4 -carbonyl, 1- aminopentyl-1 -carbonyl, valyl, 2-tert- butylglycyl, isoleucyl, leucyl, 3- cyclohexylalanyl, phenylalanyl, N- methylphenylalanyl, tetrahydrosioquinolyl-2- histidyl, 1-aminoindyl-1-carbonyl, 3- pyridylalanyl, 2-cyclohexylglycyl , norleucyl, norvalyl, neopentylglycyl , trytophanyl, glycyl, 2 , 2-dimethylglycyl, alanyl, β-alanyl and 3-naphthylalanyl residues; X is hydrogen, alkyl, cycloalkyl, -CH 2 - cyclohexyl or arylalkyl; s, t and u are independently 0 or 1; and K is hydroxy, alkoxy, phenoxy, benzyloxy or a substituted or unsubstituted amino moiety; and the salts thereof with physiologically tolerated acids ; and b) a therapeutically effective amount of a second antiarthritic drug selected from the group consisting of: a nonsteroidal antiinflammatory agent, an organic gold derivative, D- penicillamine, a 4-aminoquinoline, azathioprine, methotrexate, cyclosporin, an angiogenesis inhibitor, a monoclonal antibody to T cells, a monoclonal antibody to an adhesion molecule, a monoclonal antibody to a cytokine or growth factor, TNFR-IgG, IL-1 receptor antagonists and ICE inhibitors. 26. The composition of Claim 25 wherein the compound has the formula as defined in any one of claims 14-24. _ 27. The composition of Claim 25 or 26 for use in therapy or prophylaxis, for example, in the treatment of rheumatoid arthritis in a mammal (e.g., a human).

Inventors: BANERJEE SUBHASHIS, BARLOZZARI TERESA, HAUPT ANDREAS, BANERJEE, SUBHASHIS, BARLOZZARI, TERESA, HAUPT, ANDREAS
Applicants: BANERJEE SUBHASHIS, BARLOZZARI TERESA, BASF AG, HAUPT ANDREAS, BANERJEE, SUBHASHIS, BARLOZZARI, TERESA, BASF AKTIENGESELLSCHAFT, HAUPT, ANDREAS
Application year: 1998
Main classification: A61K38
IPC codes: A61K38, A61K39, A61K45, A61P19, A61P29, C07K7