SKIN TEST FOR SCHIZOPHRENIA

Abstract: A diagnostic method for assaying schizophrenia in a subject is provided wherein a preparation comprising platelet derived proteins or fractions thereof having a pI above about 6.5 is injected into a subject and the occurrence of delayed type hypersensitivity (DTH) reaction at the site of the injection is determined. A positive DTH reaction indicates that the tested subject has a high likelihood of being schizophrenic. The protein preparation used in the diagnostic method is also provided as well as a method for its preparation and a kit for use in the diagnosis of schizophrenia using the above method.

Description: SKIN TEST FOR SCHIZOPHRENIA FIELD OF THE INVENTION The present invention relates to diagnostic tests, and more specifically to diagnostic tests for psychiatric diseases. LIST OF REFERENCES The following is a list of references which is considered to be pertinent for the purpose of understanding the background of the invention: 1. Nyland, H., Naess, A. and Lundre, H.: Lymphocyte subpopulations in peripheral blood from schizophrenic patients. Acta Psvchiat. Scand. 1980; 61; 313-318. 2. Hirata-Hibi, M., ffig^hi, S., Tachibana, T. and Watanabe, N., Stimulated lymphocytes in schizophrenia Arch. Gen. Psvchiat. 1982; 39; 82-87. 3. Coeffey, C.E., Sullivan, J.L. and Rice, R.R., T lymphocytes in schizophrenia. Biol. Psychiatry 1983; 18; 113-119. 4. Kolyaskina, G.I. Blood lymphocytes in schizophrenia - immunological and virological aspects. Adv. Biol. Psvchiat. 1983; 12; 142-149. 5. Bessler, H., Eviatar J., Meshulan. M., Tyano. S., Djaldetti, M. and Sirota P. Theophyllin-sensitive T-lymphocyte subpopulation in schizophrenic patients. Biol. Psychiatr. 1987; 22; 1025-1029. 6. Muller, N., Ackenheil, M., Eckstein, R., Hofschuster, E. and Mempel, W. Reduced suppressor cell function in psychiatric patients. Ann. N. Y. Acad Sci. 1987; 396; 686-690. 7. Mihalovic, LJ. .and Jankovic B.D. Effects of intraventricularly injected anti-caudatus antibody on the electrical activity of the cat brain. Nature 1961 ; 192; 665. 8. Ra.ppo.rt, M.M., Karplak, S.E. and Mahadik, S.P. Biological activities of antibodies injected into the brain. Fed. Proc. 1979; 38; 2391. 9. Vartanian, M.E. Doyskina, G.S. Lozovsky, D.V., Burbaera, G.S. and Ignaton, S.A. Aspects of humoral and cellular immunity in schizophre- nia. In: Birth Defects. Original Article Series D. Bergsma and A. Goldstein, eds. vol 14; 339-364; Alan R. Liss; New York, N.Y; 1978. 10. Rotman, A. Blood platelets in psychopharmacological research. Prog Neuropsycopharmacol. 1983; 6; 135-151. 11. Pletscher, A. Biological Psychiatry, Gea Racagni, ed; Elsevier Science Publisher; 1991; 2, 354-356. 12. Shinitzky, M., Deckmann, M., Kessler, A., Sirot.a, P., Rabbs, A. and Elizur, A., Platelet autoantibodies in dementia and schizophrenia - possible implications for mental disorders. Ann. N. Y. Acad Sci. 1991; 621: 205-217. 13. Kessler, A. and Shinitzky, M., Platelets from schizophrenic patients bear autoantiboies that inhibit dopamine uptake. Psvchobiolos 1993; 21; 229-306. 14. PCT Patent Application WO 95/23970. 15. Deckmann et al, Italian Journal of Psychiatry and Behavioural Sciences, 6:29-34, 1996. The above references will be acknowledged in the text below by indicating their number from the above list shown in brackets. BACKGROUND OF THE INVENTION Schizophrenia is a syndrome which encompasses a variety of symptoms including paranoia, auditory hallucination, delusions, catatonia, bizarre behavior and emotional withdrawal. Schizophrenia affects about 1% of the total population. Its economical and social burden on society is enormous since onset occurs in youth thus requiring patients to be under medical and psychiatric supervision for most of their lives. Schizophrenia is therefore one of the most costly diseases in the industriaJ zed world. Since the biochemical basis of schizophrenia has not yet been elucidated, diagnosis today is still based solely upon psychiatric evaluation. Furthermore, no therapy is currently available for schizophrenia although the symptoms may be ameliorated by neuroleptic drugs. Many reports have implicated the immune system in the etiology and course of several mental disorders. Serum antibodies which cross-react with brain antigens have been found in the blood of schizoplirenic patients (1 , thus indicating that schizophrenia is also an autoimmune disease (7_9) . Furthermore, platelets have been used as a model for neuron tissue^ 0 ' 1 ^ and elevated levels of autoantibodies to platelets have been detected in schizophrenic and demented patients, but not in patients suffeiing from m.anic-depressive disorder, depression, personality disorders or schizoaffective disorders " \ A cellu r response against autologous platelets was also demonstrated in schizophrenia patients who showed a delayed type hypersensitivity (DTH) reaction when injected with platelets collected from their own blood (15) . It is therefore the object of the present invention to provide a test for the diagnosis of schizophrenia in a subject. SUMMARY OF THE INVENTION A novel finding in accordance with the invention is that schizophrenic patients exliibit a reaction which displays characteristics of a typical delay ed-type hypersensitivity reaction when injected with a specific, novel fraction of platelet proteins. This novel fraction is referred to herein as "Pool 2 proteins". The Pool 2 proteins, which constitute an aspect of the invention, are characterized by having an isolectric point (pi) which is above about 6.5 and preferably within the range of about 6.5 to about 9.5. When injected with Pool 2 proteins, schizopihrenic patients show such a delayed-type hypersensitivity (DTH) reaction. It is to be noted that although the reaction of the patients to the Pool 2 proteins is referred to above and below as a DTH reaction, there may be cases in which the time profile of the reaction of the patient to the Pool 2 proteins will be different than the time profile of a typical DTH reaction. The present invention thus provides a protein preparation (hereinafter "Pool 2 proteins") comprising platelet derived proteins or fractions thereof having an isoelectric point (pi) above about 6.5 and preferably within the range of above 6.5 to about 9.5, said preparation capable of eliciting a DTH reaction in a scliizoplirenic individual upon injection thereof to the individual. The present invention further provides a diagnostic method for assaying schizophrenia in a subject comprising: (a) obtaining a preparation comprising, as an active component, platelet derived proteins or fractions thereof having an isoleectric point (pi) above about 6.5 and preferably within the range of about 6.5 to about 9.5 ( "pool 2 proteins "); (b) injecting said protein preparation into a subject; and (c) examining the subject for the occurrence of a delayed type hypersensitivity reaction at the site of the injection, a positive result being a reaction above that which is observed in non-schizophrenic subjects, indicating that the subject has a high likelihood of being schizophrenic. In accordance with the invention it was also surprisingly found that the Pool 2 proteins may be either prepared from autologous platelets of the individual to be tested or, alternatively, from a pool of heterologous platelets which were obtained from a number of individuals other than the tested subject. It was also surprisingly found that, in most cases, the DTH reaction in a schizophrenic patient is substantially higher when the individual is injected with such Pool 2 proteins obtained from a pool of heterologous platelets as compared to a lower DTH reaction in the same tested individual injected with Pool 2 proteins prepared from his own autologous platelets. This finding provides the advantage of preparing a Pool 2 protein preparation which may then either be used immediately or alternatively, be stored at appropriate conditions (e.g. refrigeration) and used for various periods of time to diagnose schizophrenia in a large number of individuals. This obviates the need to repeatedly obtain a blood sample comprising platelets from the tested individual immediately prior to cauying out the diagnostic assay of the invention. Thus, by a preferred embodiment, the present invention further provides a method for the preparation of a reagent for use in diagnosis of schizophrenia, comprising: (a) obtaining blood samples from a number of individuals, preparing a pool from said samples and collecting platelets therefrom; (b) preparing a protein fraction from said platelet preparation comprising proteins or fractions thereof having a pi of above about 6.5, preferably within the range of about 6.5 to about 9.5. The Pool 2 proteins prepared from heterologous individuals may either be prepared from a number of individuals suffering from schizophrenia or, alternatively, also from a mixture of blood samples obtained from schizopahrenic patients as well as healthy individuals. The present invention yet further provides a diagnostic method for assaying schizophrenia in a subject comprising: (a) obtaining a blood sample from a number of schizophrenic and/or non schizophrenic indivuals other than the tested subject and collecting platelets therefrom; (b) preparing a protein fraction from said platelet separation comprising proteins or fractions thereof having a pi of above about 6.5, preferably within the range of above 6.5 to about 9.5; (c) injecting said protein preparation into a subject; -aid (d) examining the subject for the occurrence of a delayed type hypersensitivity reaction at the site of the injection, a positive result being a reaction above that which is observed in non-schizophrenic subjects, indicating that the subject has a high likelihood of being schizophrenic. By a preferred embodiment, a method for diagnosis of schizophrenia in an individual is provided as above wherein the protein fraction collected in stage (b) is then stored at appropriate conditions for repetitive use as described in stages (c) .and (d) above at later periods of time for a number of tested individuals. Although, as explained above, there is an advantage in preparing a pool of Pool 2 proteins from a number of individuals other than the individual to be tested, in accordance with the diagnostic method of the invention, it is also possible to use a Pool 2 preparation prepared from autologous platelets of the individual to be tested. The present invention thus provides a diagnostic method for assaying schizophrenia in a subject comprising; (a) obtaining a blood sample from an individual and collecting platelets therefrom; (b) collecting proteins or fractions thereof from said platelet sample, said proteins or fractions having a pi of above about 6.5, preferably within the range of about 6.5 to about 9.5; (c) injecting said collected proteins or fractions thereof to the tested individual; and (d) examining the subject for the occurrence of delayed type hypersensitivity reaction at the site of the injection, a positive result being a reaction above that which is observed in non-schizoplirenic subjects, indicating that the subject has a high likelihood of being schizophrenic. Typically, the Pool 2 proteins will be collected by subjecting the collected platelet proteins to isoelectric focusing wherein the proteins having a pi of above about 6.5, preferably within the .range of 6.5 to 9.5 are collected by methods known in the art. However, in accordance with the invention it has also been found that the Pool 2 protein preparation may also be prepared by methods which do not include isoelectric focusing such as, for example, by extracting the proteins from the platelet sample using for example a detergent, such extraction resulting in Pool 2 proteins having the desired pi values and capable of eliciting a DTH reaction in a schizophrenic patient. If desired, the Pool 2 protein preparation obtained by any one of the methods mentioned above may be subject to further fractionation steps, e.g. by thin layer chromatography, by high pressure liquid chromatography or by many other purification fractionation methods known, per se. Fractions thus obtained can each then be tested for activity, namely for its ability to cause the DTH reaction in schizophrenic patients. Such purified fractions, as well as individual proteins, polypeptides or peptides among the Pool 2 proteins which are active in eliciting the DTH reaction in schizophrenic patients, are also an aspect of the invention. The Pool 2 protein preparation prepared in accordance with the invention and used in the diagnostic methods of the invention include proteins purified from the Pool 2 proteins, polypeptides or peptides comprising sequences of such proteins, fractions thereof, as well as proteins, polypeptides or peptides obtained by synthesis or by genetic engineering having a sequence identical or homologous (e.g. above 70%, preferably above 80%, and typically above 90% homology) to that of the proteins of the Pool 2 proteins. In accordance with the invention, Pool 2 proteins used in the diagnostic assay of the invention are such which are capable of eliciting DTH activity in an injected individual, the DTH activity being tested by the test .known in the art. In short, the Pool 2 proteins are intradermally injected into the tested individual at the fore.arm or thigh and the reaction at the injection site is evaluated after 24, 48 and 72 hours by measuring the reaction diameter around the induration. .As mentioned above, there may be cases in which the time profile of the reaction will differ from the typical time profile of a DTH reaction. The present invention further provides a kit for use in diagnosis of schizophrenia, comprising said Pool 2 proteins, active protein fractions obtained therefrom, or individual active proteins or peptides, derived from said Pool 2 proteins. Preferably, such proteins are provided in either injectable form or in a form suitable for preparing an injectable formulation, e.g. a lyophilysate. Typically, the kit will be provided with instructions for use or a chart or pictures for guidance of the manner of scoring the results. BRIEF DESCRIPTION OF THE FIGURES Fig. 1 shows the reaction on the forearm of a schizophrenic patient which was injected with the following preparations: i. Pool 2 proteins (m.arked as P2) ii. Pool 1 proteins (marked as PI) iii. Autologous platelets (marked A) iv. PBS (marked as C = control). The invention will now be illustrated in the following examples, which are annexed to the above drawings. EXAMPLES A study on the reaction of a subject to an injection of platelets collected from his or her own blood or an injection of Pool 2 proteins was carried out in Israel at The Hospital for Mental Health in Sha'ar Menashe, The Geriatric Hospital in Pardes Hana and at the Weizmann Institute of Science. Example 1 Injection of autologous proteins 1. Preparation of platelets 10 ml venous blood was collected from a subject and centrifuged for 20 mins. at 20°C and 150 g. The supernatant containing platelets was collected and centrifuged three times for 10 min. at 20°C and 2000 g and the platelets resuspended in phosphate buffered saline (PBS) containing 5 mM EDTA. After the last washing, the platelets were resuspended in sterile PBS at a final concentration of 2x10 platelets/ml. 2. Autologous skin test and measurement of delayed type hypersensitivity (DTH reaction 0J ml of the platelet suspension obtained as above from a particular subject was injected intradermally back into the forearm of the same subject. A second injection of 0J ml PBS spaced about 10 cm from the point of sample injection served as a control. DTH reaction at the injection sites was measured in accordance with methods known in the art (Skornik, Y., et al, Cancer Immunol. Immunother. 11:93-96, 1981). Results The tests were carried out for groups subject: a) 18 healthy subjects under the age of 65. b) 10 healthy subjects above the age of 65. c) 41 schizoplirenic pateints. d) 21 demented patients. The results of this test are shown in the following Table 1. Table 1 Autologous (Self-Self) Skin Reaction Against Platelets in Humans for the Detection of Schizophrenia Summary of a Multi-Center Study As can be seen, 38 out of the 41 schizophrenic patients showed a DTH reaction, while only one healthy individual under the age of 65 out of the 18 which were tested, reacted positively. Positive reactions were not observed in 10 healthy individuals over the age of 65, as well as in 21 demented patients. Sensitivity of this test is thus 92% and the specificity is 98%. As seen in the Table, while almost all of the tested schizophrenic patients showed a DTH reaction to the Pool 2 proteins prepared from their autologous platelets, only one healthy individual had a positive DTH reaction. Thus, the diagnostic assay of the invention showed a very high sensitivity and specificity for the diagnosis of schizophrenia in a tested individual. Example 2 Preparation of Pool 1 and Pool 2 proteins Methods Platelet suspension containing about 20 gr total protein was obtained as in Example 1, and the platelets solubilized with 40 ml of a solution containing 0.5% of the detergents NP-40 and Triton-X-100 for 5 mins. at room temperature with gentle shaking. The solution was then centrifuged at 4000 g for 15 mins. at 20°C. The supernatant was collected, and the pellet was subjected to two further extractions with 10 ml 0.1% Triton-X-100. The three supernatants were combined and Bio-Lyte Ampholyte™ 3/10 (40%) of BioRad was added to a final concentration of 1%. The solubilized proteins were subjected to isoelectric focusing. 60 ml of sample was applied to the Rotofor™ system of BioRad, using 0.1 M phosphoric acid as anode solution, and 0.1 M NaOH as cathode solution. The isoelectric focusing was performed for about 4 hours at 10°C using 10 Watt constant power until the current remained constant for 30 mins. Proteins were divided into two separate groups in accordance with their pi: proteins having a pi in the range of 2-6.5 are referred to as Pool 1 proteins while proteins having a pi in the range of 6.5-10 are referred to as Pool 2 proteins. Pool 1 and Pool 2 proteins were harvested separately and diluted 1 :50 with PBS. Example 3 Injection of Pool 1 and Pool 2 proteins Method The following four preparations were injected intradermally into the forearm of a schizophrenic patient: i. Pool 2 proteins (marked as P2) ii. Pool 1 proteins (marked as PI) iii. Autologous platelets (marked A) iv. PBS. 0J ml of each above preparation were injected and the prepara- tions were injected at four different injection sites spaced about 10 cm from each other. The skin reaction at each injection site was monitored 24 h, 48 h and 72 h after injection. Results The results are seen in Fig. 1 where (i) is the highest injection site on the arm and (iv) is the lowest. As seen in the figure, a DTH response measured as explaiend above, was observed in the two schizophrenic patients at the site of injection of Pool 2 proteins and no DTH reaction was seen at the site of PI injection. Furthermore, the DTH reaction at the P2 site of injection was substantially enhanced as compared to the DTH reaction seen at the site of injection of the autologous platelets. Thus, it is, in most cases, preferred to use a P2 protein preparation obtained from a pool of blood samples obtained from several heterlogous individuals in the diagnostic assay of the invention.

Claims: CLAIMS: 1. A protein preparation comprising platelet derived proteins or fractions thereof having an isoelectric point (pi) above about 6.5 and preferably within the range of above 6.5 to about 9.5, said preparation capable of eliciting a DTH reaction in a schizophrenic individual upon injection thereof to the individual. 2. A diagnostic method for assaying schizophrenia in a subject comprising: a) obtaining a preparation comprising, as an active component, platelet derived proteins or fractions thereof having a pi above about 6.5; b) injecting said preparation into a subject; and c) exan ining the subject for the occurrence of delayed type hypersensitivity reaction at the site of the injection, a positive result being a reaction above that which is observed in non-schizophrenic subjects, indicating that the subject has a high likelihood of being schizoplirenic. 3. A method for the preparation of a reagent for use in diagnosis of schizophrenia, comprising: a) obtaining blood samples from a number of individuals, preparing a pool from said samples and collecting platelets therefrom; b) preparing a protein fraction from said platelet preparation comprising proteins or fractions thereof having a pi of above about 6.5. 4. A diagnostic method for assaying schizophrenia in a subject comprising: (a) obtaining a blood sample from a number of schizophrenic and/or non schizophrenic indivuals other than the tested subject and collecting platelets therefrom; (b) preparing a protein fraction from said platelet separation comprising proteins or fractions thereof having a pi of above about 6.5; (c) injecting said protein preparation into a subject; and (d) examining the subject for the occurrence of a delayed type hypersensitivity reaction at the site of the injection, a positive result being a reaction above that which is observed in non-schizop.hrenic subjects, indicating that the subject has a high likelihood of being schizophrenic. 5. A diagnostic method for assaying schizophrenia in a subject comprising; a) obtaining a blood sample from an individual and collecting platelets therefrom; b) collecting proteins or fractions thereof from said platelet sample, said proteins or fractions having a pi of above about 6.5. c) injecting said collected proteins or fractions thereof to the tested individual; and d) examining the subject for the occurrence of delayed type hypersensitivity reaction at the site of the injection, a positive result being a reaction above that which is obse.rved in non-schizophrenic subjects, indicating that the subject has a high likelihood of being schizoplirenic. 6. The method of any one of the previous claims, wherein said proteins or fractions thereof have a pi within the range of above 6.5 to about 9.5. 7. A kit for use in diagnosis of schizophrenia comprising a protein or a fraction thereof prepared from human platelets, said proteins or fractions thereof having a pi of above about 6.5 together with instructions for use. 8. A kit in accordance with Claim 6, wherein the proteins or fractions thereof have a pi within the range of above 6.5 to about 9.5. . . 9. A kit in accordance with Claims 6 or 7, wherein the proteins or fractions thereof are prepared from heterologous platelets obtained from a number of individuals other than the individual to be tested. 10. A kit in accordance with Claims 6 or 7, wherein the proteins or fractions thereof are prepared from autologous platelets obtained from the individual to be tested.

Inventors: DECKMANN MICHAEL, SHINITZKY MEIR, DECKMANN, MICHAEL, SHINITZKY, MEIR
Applicants: DECKMANN MICHAEL, SHINITZKY MEIR, YEDA RES & DEV, DECKMANN, MICHAEL, SHINITZKY, MEIR, YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Application year: 1998
Main classification: G01N33
IPC codes: A61K49, G01N33