CALIXPYRROLES, CALIXPYRIDINOPYRROLES AND CALIXPYRIDINES

Abstract: The present invention provides calixpyrrole, calixpyridinopyrrole, and calixpyridine macrocycles, having 4, 5, 6, 7, or 8 heterocyclic rings, as well as syntheses, derivatives, conjugates, multimers, and solid supports thereof. Such macrocycles have proved to be effective and selective ion- and neutral molecule-binding agents forming supramolecular ensembles, and ion- and neutral molecule-separation agents. The macrocycles are fully meso-non-hydrogen-substituted porphyrinogens, a few molecules of which were previously known but not recognized as possessing anion- or molecule-binding properties. The binding mode is noncovalent, primarily that of hydrogen-bonding, thereby providing a new mode for liquid chromatography, that of Hydrogen Bonding Liquid Chromatography. Further useful applications of the macrocycles provided herein include environmental remediation by removal of undesired ions or neutral molecules, and removal of phosphate for kidney dialysis.

Description: DESCRIPTION CALIXPYRROLES, CALIXPYRTOINOPYRROLES AND CALIXPYRIDINES BACKGROUND OF THE INVENTION The present invention relates to ion and neutral molecule binding, separation, or detection, using calix[n]pyrrole, calix[m]pyridino[n]pyrrole or calix[m]pyridine macrocycles. Anions play essential roles in biological processes; indeed, it is believed that they participate in 70% of all enzymatic reactions. There is, therefore, intense effort being devoted to the problem of anion complexation and recognition. In the molecular recognition arena, a number of research groups have followed Nature's lead and have designed and synthesized receptors that use hydrogen bonds alone, or in concert with electrostatic interactions, to coordinate to anions. Nonetheless, there remains at present a critical need for additional anion complexing agents that are either easy to make or inherently selective in their substrate binding properties. Additionally, the separation of anionic mixtures using chromatographic techniques involving anion binding interactions is a little studied area of chemistry. Current techniques for purification of anions such as oligonucleotide fragments, polyphosphate-containing molecules such as ATP, ADP and AMP, carboxylates, or N-protected amino acids involve derivatization prior to separation, leading to decreased yields and cumbersome methodology, or involve a salt- separation step following chromatography. This is a very important area to both scientists and clinicians as both mono- and di-nucleotides, natural and synthetic oligonucleotides play critical roles in modern biotechnology as well as medicine. Oligonucleotides are used, for instance, as hybridization probes in blot analyses, primers for PCR amplification, and for site-specific mutagenesis. Furthermore, in some areas, oligonucleotide-derived products are currently being used as probes for the detection of genetic diseases and for proviral HIV, the causative agent of Acquired Immunodeficiency Syndrome (AIDS). Oligonucleotides are also being considered as potential chemotheraputic agents, both directly, i.e., in gene therapy, and in an antisense fashion. The above-mentioned applications require oligonucleotide materials of impeccable purity (often greater that > 99.99%). Such purity, however, is not readily obtained using existing technology. Presently, gene products and other oligonucleotide-type materials are purified using polyacrylamide gel electrophoresis (PAGE). This approach suffers from the requirement of using toxic materials and is painfully manpower-demanding and low-yielding. Liquid chromatographic techniques, in particular, high performance liquid chromatography (HPLC) and High Performance Affinity Chromatography (HPAC) employing speciality silica gels are currently used to separate biological molecules. Indeed, silica gel phases with bonded groups, such as linear hydrocarbons, amino groups, cyano-groups, carboxylic amides and amino acids, are all known. Unfortunately, few, if any, of these phases are efficacious for the efficient, high-yielding separation of nucleotides and oligonucleotides. Those that work best for this purpose are ion exchange columns which, on a limited basis, can sometimes separate oligonucleotides containing 40 or fewer residues. However, this technique still suffers from several limitations, including the requirement for severe conditions, such as elution at pH 2.7, for routine operation. The use of high concentration buffers (greater than IM) and gradients that often include formic acid or formamide, also limits the half-lives of ion exchange columns. Reverse phase columns use column media, such as silica gel with appended groups such as alkyl chains, that separate species on the basis of hydrophobic effects. Reverse phase columns may be used at pressures up to 5000 psi. However, in order to separate species such as oligonucleotides on this type of column, protecting groups must first be appended to the oligonucleotide, and ion-pairing reagents must be used, requiring an additional purification step after the chromatographic separation. A type of stationary phase has been previously described in which purine and pyrimidine bases are bonded to silica gel. With this support, base-pairing interactions between the nucleic acid base pairs and the modified silica gel were expected to improve the resolution obtained in nucleic acid separation procedures. Although such stationary phases were used to separate nucleic acid-free bases, purine alkaloids, nucleosides, and mono- and oligonucleotides, this approach unfortunately has demonstrated the least success in the case of oligonucleotide separation, which is the most important area. Thus, prior to the present invention, there remained a critical need for improved solid supports that would effectively separate nucleotides and oligonucleotides. Additionally, sapphyrin (an expanded porphyrin known to bind anions) has been attached to a functionalized silica gel. The separatory properties of this material were not sufficient to separate oligonucleotides successfully due to broad peaks observed for (3- to 9- mer) oligonucleotide mixtures. Furthermore, synthetic challenges are associated with preparing the requisite functionalized sapphyrins since they require over 20 synthetic steps to produce. Current technology for dialysis in medical applications relies on membranes, such as microfiltering cellophane, to filter anions such as chloride anion or phosphate-containing anions from the blood stream. Aluminum hydroxide or calcium carbonate cocktails must be consumed by the dialysis patient in order to bind the anionic species. A major drawback of this technology is that aluminum builds up in cellular membranes to toxic levels over time causing ailments including dementia and death. Calcium carbonate offers a less toxic substitute, however, it is less efficient and is associated with hypercalcemia. Water-soluble anion binding agents are desired as drug delivery agents. For example, many anti-viral drugs only show activity when phosphorylated. However, many phosphorylated drug derivatives are too polar to pass tnrough cell wall membranes. A water- soluble anion binding agent may be able to encapsulate the negative charge and so allow the drug to pass through cell walls. The synthesis of new molecular devices designed to sense and report the presence of a particular substrate is an area of analytical chemistry that is attracting attention. The detection of anionic species is a particular challenge, as anions are difficult to bind and are generally larger than cations leading to a smaller charge-to-radius ratio. Molecular recognition of neutral compounds presents a challenge in the area of supramolecular chemistry. Binding of substrates, such as short-chain alcohols and simple monoamides, is particularly difficult because these molecules have few functionalized sites available for hydrogen bonding, and they lack the large hydrocarbon surfaces necessary to participate in efficient hydrophobic or π-π stacking interactions. Association constants for neutral substrate-synthetic receptor complexes are thus generally modest, even though the architectural complexity ofthe receptors is often high. Cation binding agents may be useful as sensors for particular cations or as sequestering agents. Additionally, particular cation-complexes may be useful in medicine as imaging agents or in the treatment of disease. One aspect of the present invention involves calixpyrroles. Calixpyrroles represent a subset of a class of macrocycles that was previously termed porphyrinogens. Porphyrinogens are non-conjugated macrocyclic species composed of four pyrrole rings linked in the oc-position via sp 3 hybridized carbon atoms. Porphyrinogens that carry me so -hydrogen atoms are prone to oxidation to the corresponding porphyrins. Fully rnt?-fo-non-hydrogen-substituted porphyrinogens are generally stable crystalline materials. The first such macrocycle, meso- octamethylcalix[4]pyrrole, was reported over a century ago by Baeyer (Ber. Dtsch. Chem. Ges. 1886, 19, 2184) using a condensation between acetone and pyrrole catalyzed by hydrochloric acid, however, the structure of the molecule was not elucidated. This method was reportedly refined by Dennstedt and Zimmerman (Ber. Dtsch. Chem. Ges. 1887, 20, 850) by replacing the hydrochloric acid catalyst with "chlorzink" (presumably zinc chloride) and heating the reaction. Chelintzev and Tronov reportedly produced calix[4]pyrroles by the method of condensing acetone and pyrrole, methylethyl ketone and pyrrole, methylhexylketone and pyrrole and a mixture of acetone and methy lethy lketone with pyrrole (J. Russ. Phys. Chem. Soc. 1916, 48, 1 197; Chem Abstr. 1917, 11, 1418). Chenlintzev, Tronov and Karmanov reported further production of calixpyrroles by condensing cyclohexanone with pyrrole and a mixture of acetone and cyclohexanone with pyrrole (J. Russ. Phys. Chem. Soc. 1916, 48, 1210). Rothemund and Gage reportedly refined Dennstedt and Zimmerman's method by replacing the acid catalyst with methanesulfonic acid (J. Am. Chem. Soc. 1955, 77, 3340). Brown, Hutchinson and MacKinnon (Can. J. Chem. 1971, 49, 4017) reportedly repeated the synthesis of meso- tetracyclohexylcalix[4]pyrrole and assigned a tetrameric macrocyclic structure. J.-M. Lehn and co-workers have reportedly synthesized me.so-octa-3-chloropropylcalix[4]pyrrole by an unpublished procedure and converted it to meso-octa-3-cyanopropylcalix[4]pyrrole (B. Dietrich, P. Viout and J.-M. Lehn in Macrocyclic Chemistry, VCH Publishers, Weinheim, 1993, pg 82). The metal cation binding of deprotonated calix[4]pyrrole macrocycles has been studied by Floriani and co-workers (Chem. Commun. 1996, 1257). Floriani has reportedly developed a method for expanding the pyrrole rings of metal-bound deprotonated calix[4]pyrroles forming calix[l]pyridino[3]pyrroles and calix[2]pyridino[2]pyrroles (J. Am. Chem. Soc. 1995, 1 17, 2793). A further prior art method reports using pyrrole, a C4-C6 saturated alicyclic ketone and an acid containing vinyl groups or triple bonds to form a polymerized resin (WO 93/13150). In this case, the resulting products are undefined since it appears to be unknown where the modifying group is attached to the product. While the term porphyrinogen is now widely accepted in the literature, the present inventors believe the fully mesø-non-hydrogen-substituted systems are misnamed. They are not bonafide precursors of the porphyrins and might, therefore, be better considered as being calixpyrroles rather than porphyrinogens. Such a renaming has precedent in the chemistry of other heterocyclic ring systems. Interestingly, while related functionalized calixarenes, a class of molecules containing phenol (as opposed to pyrrole) subunits have been shown to be capable of binding anions, unmodified calixarene frameworks show no affinity for anionic guests, demonstrating that the anion-binding properties are not an inherent part of the calixarene ring structure. While a few other fully meso-non-hydrogen-substituted porphyrinogens are known, no one prior to the present inventors recognized their properties and consequent uses as described herein. SUMMARY OF THE INVENTION The present invention results from the inventors' discovery that calix[4]pyrrole macrocycles bind anions in the solid state. That discovery led the present inventors to synthesize novel calix[n]pyrroles, calix[m]pyridino[n]pyrroles or calix[m]pyridine macrocycles, to develop new methods of making said macrocycles, and to demonstrate methods of using said macrocycles as presented herein. "Calix[n]pyrrole," as used herein, means a macrocycle having "n" pyrrole rings linked in the α positions via sp- hybridized meso-carbon atoms that are not bound to hydrogen (protium), deuterium or tritium atoms. This is a subset of a class of molecules that was previously termed porphyrinogens. "CaIix[m]pyridino[n]pyrrole," as used herein, means a macrocycle having "m" pyridine rings and "n" pyrrole rings linked in the α positions via sp3 hybridized meso-carbon atoms that are not bound to hydrogen (protium), deuterium or tritium atoms. "Calix[m]pyridine," as used herein, means a macrocycle having "m" pyridine rings linked in the α positions via sp hybridized meso-carbon atoms that are not bound to hydrogen (protium), deuterium or tritium atoms. A "calix[n]pyrrole", "calix[m]pyridino[n]pyrrole" or "calix[m]pyridine" is different from a porphyrinogen since a porphyrinogen has one or more sp3 hybridized me_?o-carbon atoms bound to a hydrogen (protium), deuterium or tritium atom. A "calix[n]pyrrole'\ "calix[m]pyridino[n]pyrrole" or "calix[m]pyridine" is different from a porphomethene since a porphomethene contains three sp3 hybridized mesø-carbons and one sp2 hybridized meso- carbon. A "calix[n]pyrrole", "calix[m]pyridino[n]pyrrole" or "calix[m]pyridine" is different from a phlorin since a phlorin contains one sp3 hybridized meso-caxbon and three sp2 hybridized meso-caxbons. A "calix[n]pyrrole", "calix[m]pyridino[n]pyrrole" or "calix[m]pyridine" is different from a porphyrin since a poφhyrin contains four sp2 hybridized meso-carbons and is aromatic. A "calix[n]pyrrole", "calix[m]pyridino[n]pyrrole" or "calix[m]pyridine" is different from an expanded poφhyrin since an expanded poφhyrin contains at least one sp2 hybridized meso-cdxbon. Macrocycles of the present invention have unexpected properties that make them particularly useful. Calix[n]pyrroles and calix[m]pyridino[n]pyrroles bind anions and neutral molecular species in solution and in the solid state in such an effective and selective way that the anions or neutral molecular species can be separated from other anions and neutral molecular species. Calix[m]pyridino[n]pyrroles and calix[m]pyridines can also be used to bind and separate cations. Further, the affinity a macrocycle has for a particular species can be "tuned" by strategic choice of electron-donating or electron-withdrawing peripheral substituents for synthesis of the macrocycle. Additionally, the affinity a calix[m]pyridino[n]pyrrole or calix[m]pyridine has for a particular species can be "tuned" by N-protonation or quaternization by /' -alkylation. Applications of these properties for removal of biological ions or neutral molecule species for medical uses, or removal of undesirable ions or neutral molecule species from environmental sources provide only a few of the practical and important uses for the present molecules. A calix[n]pyrrole, calix[m]pyridino[n]pyrrole, or calix[m]pyridine macrocycle noncovalently-complexed to a molecular or ionic species is an embodiment of the present invention. When the macrocycle is a calix[4]pyrrole the ionic species is other than a metal cation. The term "n" refers to the number of pyrrole rings in the calixpyrrole macrocycle and, for molecules ofthe present invention, is 4, 5, 6, 7, or 8. The term "m+n" refers to the number of pyridine and pyrrole rings in the calixpyridinopyrrole macrocycle respectively and, for molecules of the present invention, is 4, 5, 6, 7, or 8. The term "m" refers to the number of pyridine rings in the calixpyridine macrocycle and, for molecules ofthe present invention, is 4, 5, 6, 7, or 8. "Noncovalently-complexed to a molecular or anionic species," as used herein, means that bound molecules are held to the core of a macrocycle by noncovalent binding to one or more pyrrolic N-H or pyridino N-H + groups thus forming a supramolecular ensemble. "Noncovalent binding" includes intermolecular interactions such as hydrogen bonds, dipole- dipole interactions, dipole-induced dipole interactions, ion-dipole interactions, ion pairing, van der Waals interactions, London dispersion forces, π-π stacking interactions, edge-to-face π- interactions, cation-π interactions, charge transfer interactions, or entropic, hydrophobic or solvophobic effects. In a preferred embodiment ofthe invention, the ionic species is an anionic species. "Supramolecular" as used herein describes the chemistry of complexes, that is molecular ensembles containing more than one atomic, ionic, or molecular component. Thus complexes of the macrocycles of the present invention and an ion or neutral molecule are considered to be supramolecular complexes or ensembles. A calix[n]pyrrole, calix[m]pyridino[n]pyrrole or calix[m]pyridine macrocycle attached to a solid support, where m, m+n and n are defined as herein above, is a particularly useful embodiment of the present invention. Due to the effective and selective separation of a variety of molecules by calix[n]pyrrole-derivatized solid supports as described in the examples herein, the present inventors have developed a new type of liquid phase chromatography termed "Hydrogen-Bonding Liquid Chromatography." The bases of the chromatography are noncovalent interactions, primarily hydrogen bonding. A calix[n]pyrrole, calix[m]pyridino[n]pyrrole or calix[m]pyridine macrocycle having a first conformation in a solid state when unbound to a molecular or anionic species and having a second conformation in a solid state when bound to a molecular or anionic species where n is 4, 5, 6, 7, or 8 is another embodiment ofthe present invention. In particular, the present inventors have observed an unbound 1 ,3-alternate "first" conformation, and a bound cone or 1 ,2-altemate "second" conformation for calix[4]pyrrole macrocycles. Exemplary calix[n]pyrrole macrocycles for anion and neutral molecular species binding, for solid supports, for derivatives, for multimers, for conjugates and the like, have structure I. For structure I, when n is 4, p = q = r = s = 0, R j - Ri g are independently substituents as listed in paragraph i) below, and R^ - Rp j are independently substituents as listed in paragraph ii) below. When n is 5, p = 1, q = r = s = 0, R j to R20 are independently substituents as listed in paragraph i) below, and R^ - Rg are independently substituents as listed in paragraph ii) below. When n is 6, p = q = 1, r = s = 0, R j to R24 are independently substituents as listed in paragraph i) below, and R^ - Rp are independently substituents as listed in paragraph ii) below. When n is 7, p = q = r = 1, s =0, R j to R28 are independently substituents as listed in paragraph i) below, and R^ - RQ are independently substituents as listed in paragraph ii) below. When n is 8, p = q = r = s = 1 , R j to R32 are independently substituents as listed in paragraph i) below, and R^ - RJ_J are independently substituents as listed in paragraph ii) below; i) hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, tetrahydrothiapyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester, a site-directing molecule, a catalytic group, a reporter group, a binding agent, or a couple that is coupled to a site-directing molecule, to a catalytic group, to a reporter group, or to a binding agent; ii) hydrogen, alkyl, aminoalkyl, carboxy alkyl, carboxyamide alkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, halo alkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl. Alternatively, at least two substituents attached to a pyrrole β-carbon, a meso-carbon or a pyrrole nitrogen are coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined herein in paragraph i) or ii). Odd-numbered R-substituents are other than hydrogen for macrocycles having structure I. A w&so-substituted calix[n]pyrrole, where n is 4, 5, 6, 7, or 8, and where the meso- substitution is an oxygen-, sulfur-, or alkenyl-containing substituent is an embodiment of the present invention. Oxygen-, or sulfur-containing substituents are particularly useful for giving the macrocycle a functionality that can be further derivatized for uses described herein. A "monohook" calix[n]pyrrole having an oxygen-containing carboxyl group has been found to possess self-assembly properties as shown in FIG. 4. A calix[n]pyrrole having a vinyl substituent as an example of an alkenyl group is particularly useful for forming a polymer of defined linkage and structure. Such a polymer is useful for a selective electrode as described herein. A further aspect ofthe present invention is a β-substituted calix[n]pyrrole. Exemplary β- substituted calixfn] pyrrole macrocycles have structure I as described herein wherein at least one even-numbered R substituent is other than hydrogen. Substitution on a β-carbon of a pyrrole ring provides even further flexibility in the design of macrocycles of the present invention for the various uses described herein. A calix[m]pyridino[n]pyrrole macrocycie having structure II is a further aspect ofthe present invention. The term "m" designates a number of pyridines, and the term "n" designates a number of pyrroles, in macrocycle II, m + n = 4, and m is other than 1 or 2. When m is 4; n = 0, p =1, and q= 0, RlOl to Rl08 and R201 to R212 are independently substituents as listed in paragraph i) above, and R301 - R304 are independently substituents as listed in paragraph ii) above. When m is 3; n = 1, p = 0, q = 1, RlOl to R108 and R201 to R211 are independently substituents as listed in paragraph i) above, and R301 - R304 are independently substituents as listed in paragraph ii) above. are ot her than hydrogen. When R301 -R304 is other than a lone pair of electrons, Z = 1 , that is, the pyridine nitrogen atom is positively charged. When R301-R304 is a lone pair of electrons Z = 0, that is, the pyridine nitrogen atom is neutral. Further aspects ofthe present invention include calix[m]pyridino[n]pyrrole macrocycles where m and n designate a number of pyridines and pyrroles in the macrocycle, respectively, m and n are other than 0, and m + n = 5, 6, 7, or 8. Each pyridine or pyrrole α-carbon is bound to another pyridine or pyrrole α-carbon via one non hydrogen-linked sp 3 -hybridized me o- carbon; each sp-^ hybridized rnejo-carbon is further independently bonded to a group characterized in paragraph i) above with the exception of hydrogen; each pyridine β carbon, pyrrole β carbon and pyridine γ carbon is independently bonded to a group characterized in paragraph i) above; each pyridine or pyrrole nitrogen is bound to a group characterized in paragraph ii) above. In a further embodiment, at least one sp 3 meso-carbon, pyridine β-carbon, pyrrole β-carbon, pyridine γ-carbon, pyrrole nitrogen, or pyridine nitrogen is coupled to form a bridged structure to itself or to another sp 3 hybridized meso-carbon, pyridine β-carbon, pyrrole β-carbon, pyridine γ carbon, pyrrole nitrogen, or pyridine nitrogen; and when coupled to form a bridged structure, non-bridged atoms are as defined for an sp 3 hybridized meso-carbon, pyridine β-carbon, pyrrole β-carbon, pyridine γ carbon, pyrrole nitrogen, or pyridine nitrogen. A calix[m] pyridine macrocycle having structure III wherein m is 4, 5, 6, 7, or 8 is a further aspect of the present invention. When m is 4, p = q = r = s = 0, Rioi to Ri \2 and R201 to R208 are independently substituents as listed in paragraph i) below, and R301 - R304 are independently substituents as listed in paragraph ii) above. When m is 5, p = 1, q = r = s = 0, RlOl to Ri 15 and R201 to R210 are independently substituents as listed in paragraph i) above, and R301 - R305 are independently substituents as listed in paragraph ii) above. When m is 6, p = q = 1 , r = s = 0, RlOl to Ri 18 and R201 to R212 are independently substituents as listed in paragraph i) above, and R301 - R3O6 are independently substituents as listed in paragraph ii) above. When m is 7, p = q = r = 1, s = 0, Rioi to R121 and R20I to R214 are independently substituents as listed in paragraph i) above, and R301 - R307 are independently substituents as listed in paragraph ii) above. When m is 8, p = q = r = s = 1, RlOl to R124 and R201 to R216 are independently substituents as listed in paragraph i) above, and R301 - R308 are independently substituents as listed in paragraph ii) above. R201-R216 are other than hydrogen. When R301-R30m are other than a lone pair of electrons, Z = 1 , that is the pyridine nitrogen atom carries a positive charge. When R301-R30m are a lone pair of electrons Z = 0, that is the pyridine nitrogen atom is neutral. In a further embodiment at least two substituents are coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined in paragraph i) or ii) above. A calixfnjpyrrole where n is 5, 6, 7, or 8 is another embodiment of the present invention. Calix[n]pyrroles having a number of pyrrole rings greater than four have not been previously known. An advantage of the larger macrocycles over those having four pyrroles is that they are expected to show enhanced binding properties with large- or poly-anions and have the property of binding more than one anion simultaneously. A chiral calixfnjpyrrole, calixfmjpyridinofnjpyrrole, or calixfm jpyridine where m and n are defined herein above, is a further embodiment of the present invention. A chiral calixpyrrole, calixpyridinopyrrole or calixpyridine is expected to have different binding properties for each of a pair of enantiomeric species, thus allowing preferential binding of a particular enantiomer. The present invention provides a number of different novel methods of making calixfnjpyrrole, calixfmjpyridinofnjpyrrole, and calixfmjpyridine macrocycles. A first method of making embodiment is a method of making a calixfnjpyrrole where n is 4, 5, 6, 7, or 8, the calixfnjpyrrole having an ester substituent, comprising reacting an ester-functionalized ketone, pyrrole and a ketone to produce a calixfnjpyrrole having an ester substituent. A second method of making embodiment is a method of making a calixfnjpyπole where n is 4, 5, 6, 7, or 8, the calixfnjpyrrole having an acid substituent, comprising reacting an ester- functionalized ketone, pyrrole and a ketone to produce a calixfnjpyrrole ester derivative; and de-esterifying the ester derivative to produce a calixfnjpyrrole monoacid derivative. A method of making a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising activating a calixfnjpyrrole where n is 4, 5, 6, 7, or 8; and reacting the activated calixfnjpyrrole with an electrophile is a third method of making embodiment ofthe invention. A fourth synthetic method embodiment of the invention is a method of making a β- substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising reacting a β-substituted pyrrole and a ketone to produce a β-substituted calixfnjpyrrole. A method of making a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising producing a calixfnjpyrrole radical where n is 4, 5, 6, 7, or 8; and reacting the radical with a radical acceptor molecule is a fifth synthetic method embodiment ofthe invention. A method of making a calixfnjpyrrole wherein n is 5, 6, 7, or 8 comprising reacting a pyrrole and a ketone in the presence of a Lewis acid is a sixth synthetic method embodiment of the invention. A seventh method of making embodiment is a method of making a calixfnjpyrrole wherein n is 4, 5, 6, 7, or 8 comprising reacting a pyrrole and a ketone in the presence of a heterogenous catalyst. An eighth synthetic method embodiment of the invention is a method of making a calixfnjpyrrole wherein n is 4, 5, 6, 7, or 8 comprising the steps of a) reacting a ketone- functionalized pyrrole and an alkyl metal to form a pyrrole alcohol, b) condensing the pyrrole alcohol to form a cyclizable pyπole oligomer, and c) repeating step b) at least n minus 2 times to form a calixfnjpyrrole. A ninth synthetic method embodiment of the invention is a method of making a calixfnjpyrrole wherein n is 4, 5, 6, 7, or 8 comprising reacting a pyrrole and a ketone in the presence of an anion template. A method of making a solid-supported calixfnjpyrrole, solid-supported calixfmjpyridinofnjpyrrole, or solid-supported calixfmjpyridine comprising attaching a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine having a functionalized group to a solid support, the solid support reactive with the functionalized group, or to a tether-functionalized solid support, the tether reactive with the functionalized group is a tenth synthetic method embodiment of the present invention. A method of making a calixpyridinopyrrole comprising adding halocarbene to a calixpyrrole in a cycloaddition reaction, thereby converting a pyrrole to a pyridine and forming a calixpyridinopyrrole is an eleventh synthetic embodiment of the present invention. Preferably, the halocarbene is chlorocarbene and most preferably, is dichlorocarbene. A method of making a calixpyridine comprising: adding halocarbene to calixpyrrole or to calixpyridinopyrrole in a cycloaddition reaction so as to convert pyrrole to pyridine to form a calixpyridine is a twelth synthetic method embodiment of the present invention. The halocarbene is as described herein. A thirteenth method of making embodiment or synthesis embodiment is a method of making a calixfnjpyrrole where n is 5, 6, 7, or 8, the calixfnjpyrrole having different substituents at me5o-carbon atoms, comprising reacting pyrrole and at least two ketone molecules to produce a calixfnjpyrrole having different substitutents at me-fo-carbon atoms. A further composition of matter of the present invention is a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine made by one of the first through thirteenth synthetic method embodiments provided herein. A method of forming a complex of a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole, and an ion or a neutral molecule comprising contacting the calixfnjpyrrole or the calixfmjpyridinofnjpyrrole with the ion or neutral molecule under conditions effective to allow the formation of the complex is a further aspect of the invention. Another aspect of the invention is a method for separating a first molecule, a first anion or a first cation from a mixture of a first molecule, a first anion or a first cation and other species, comprising obtaining a calixfnjpyrrole-, a calixfmjpyridinofnjpyrrole- or a calixfmjpyridine-derivatized solid support; and contacting the solid support with the mixture to separate the first molecule, the first anion or the first cation. Another method of separating includes batch processing where a macrocycle or macrocycle of the present invention attached to a solid support is added to a mixture containing the ion or molecule to be separated, and separating the macrocycle-molecule, macrocycle-ion, solid support-macrocycle-ion or solid support-macrocycle-molecule complex. Macrocycles provided herein are particularly effective as separation media as described in the present examples. A method of transporting a molecular or ionic species through a membrane comprising incoφorating a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole into the membrane; and contacting the membrane with the molecular or ionic species in the presence of a gradient ofthe molecular or ionic species or counter gradient of another back-transported species is an embodiment of the invention. Thus, the present invention maybe used to facilitate the transport of ionic or neutral substrates in both a synport or antiport sense. A further embodiment of the present invention is an electropolymerizable calixfnjpyrrole, calixfmjpyridinofnjpyrrole, or calixfmjpyridine, which macrocycles, are useful for constituting modified electrodes for the detection of ionic or molecular species. A further embodiment ofthe present invention is an anion-, cation-, or neutral molecule- selective electrode comprising a conductive body, a polymer, and a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine. The calixfnjpyrrole, the calixfmjpyridinofnjpyrrole, or the calixfmjpyridine may be electropolymerized and form the conductive body. A method of electrochemical detection of an anion, a cation, or a neutral molecule comprising assembling an anion-, cation-, or neutral molecule-selective electrode and contacting the electrode with a solution of the anion, the cation, or the neutral molecule, and detecting the presence or absence of the anion, the cation, or the neutral molecule is an embodiment of the present invention. A method of binding a cation comprising contacting the cation with a calixfnjpyrrole or calixfmjpyridinofnjpyrrole having a cation-binding functionality, or with a calixfmjpyridine is also an aspect ofthe invention. A method of removal of pertechnetate from pertechnetate-containing nuclear waste comprising contacting the waste with a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole to form a calixfnjpyrrole- or a calixfmjpyridinofnjpyiTole-pertechnetate complex; and removing the complex from the waste is also an aspect of this invention. A method of removal of an environmental pollutant from an environmental source, comprising contacting the environmental source with a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole to form a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-pollutant complex, and removing the complex from the environmental source is also an aspect of this invention. Further embodiments of the invention include a chromatography column or a sensor comprising a solid support bound to a calixfnjpyrrole where n is 4-8, to a calixfmjpyridinofnjpyrrole where m+n=4-8 and m is not 1 or 2, or to a calixfmjpyridine where m is 4-8. Use of a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine in the preparation of a pharmaceutical composition for use in in vivo or ex vivo treatment of body tissues is another embodiment of the invention. The treatment may involve binding, transport, and/or removal of ions or neutral molecular species for conditions such as gout, for kidney dialysis, for removal of viruses, for introduction of antiviral drugs, or the like. A method, therefore, includes administering to a patient in need thereof a therapeutically effective amount of a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine. BRIEF DESCRIPTION OF THE DRAWINGS. FIG. IA illustrates the X-ray crystal structure of me5ø-octamethylcalix[4]pyrrole, 1, adopting a 1,3 -alternate conformation. Thermal ellipsoids are at 30% probability level. FIG. I B illustrates the X-ray crystal structure of m e s o - tetraspirocyclohexylcalixf4jpyrrole, 2, adopting a 1,3-alternate conformation. Thermal ellipsoids are at the 30% probability level. FIG. 2A illustrates the X-ray crystal structure of me-fo-octamethylcalix[4]pyιτole tetra- butylammonium chloride complex, 1-Cl". The me5o-octamethylcalixf4]pyrrole adopts a cone conformation wherein all four pyrrole NH groups are hydrogen-bonded to the bound chloride anion. The counter cation is omitted for clarity. Thermal ellipsoids are at the 30% probability level. FIG. 2B illustrates the X-ray crystal structure of m e s o - tetraspirocyclohexylcalix[4jpyrrole tetrabutylammonium chloride complex, 2-C1". The meso- tetraspirocyclohexylcalixf4]pyrrole adopts a cone conformation wherein all four pyrrole NH groups are hydrogen-bonded to the bound chloride anion. The counter cation is omitted for clarity. Thermal ellipsoids are at the 30% probability level. FIG. 3A illustrates the X-ray crystal structure of me.so-octamethylcalix[4jpyrrole bis- methanol complex, 1-2 MeOH. The meso-octamethylcalixf4jpyπole adopts a 1,3-alternate conformation wherein two NH groups bind to each methanol. Thermal ellipsoids are at the 30% probability level. FIG. 3B illustrates the X-ray crystal structure of /ne,sø-octamethylcalixf4]pyrroIe bis- dimethylformamide complex, 1-2 DMF. The me θ-octamethylcalixf4]pyrrole adopts a 1,2- altemate conformation wherein two NH groups bind to each dimethylformamide. Thermal ellipsoids are at the 30% probability level. FIG. 3C illustrates the X-ray crystal structure of me_fo-octamethylcalixf4]pyrrole dimethylsulfoxide complex, 1 DMSO. The we50-octamethylcalixf4]pyrrole adopts a 1,3- alternate conformation wherein one NH group binds to a dimethylsulfoxide. Thermal ellipsoids are at the 30% probability level. FIG. 4 illustrates the X-ray crystal structure of tetrabutylammonium salt of calix[4jpyrrole, 4. Calix[4jpyrrole anions self-assemble in the solid state forming dimers. Thermal ellipsoids are at the 30% probability level.. Counter cations are omitted for clarity. FIG. 5 illustrates the X-ray crystal structure of a meso-octabenzylcalix[4Jpyrrole acetone complex, 6*3(acetone). The octabenzylcalixf4jpyrrole adopts a 1 ,3-alternate conformation wherein one NH group binds to an acetone. An unbound acetone molecule in the crystal lattice is omitted for clarity. Thermal ellipsoids are at the 30% probability level. FIG. 6 illustrates the X-ray crystal structure of weΛO-tetrakis-tetrahydrothiopyranyl- calix[4jpyrrole, 7, adopting a 1,3-alternate conformation. Thermal ellipsoids are at the 30% probability level. FIG. 7 illustrates an X-ray crystal structure of β -octab romo-m ejo - octamethylcalix[4jpyrrole, 14, adopting a 1,3-alternate conformation. Thermal ellipsoids are at the 30% probability level. FIG. 8 illustrates an X-ray crystal structure of a me-fo-tetramethylcalix[4jpyrrole-p-/er/- butylcalix[4jarene pseudo dimer, 15. Thermal ellipsoids are at the 30% probability level. FIG. 9 illustrates a ' H NMR of a rne.ϊo-tetramethylcalix[4jpyrrole-p-/erf- butylcalix[4]arene pseudo dimer in CD2CI 2 . FIG. 10 illustrates a sodium chloride complex of calix[4]pyrrole-crown ether conjugate, 32. FIG. I IA illustrates a cyclic voltammogram of compound, 33, conducted in dichloromethane at a scan rate of 100 mV/sec. Working electrode: platinum disk, counter electrode: platinum wire, reference electrode: Ag/AgCl. 0.1 M tetrabutylammonium hexafluorophosphate as base electrolyte. Scale equals 2 microamps. FIG. 1 IB illustrates a cyclic voltammogram of compound, 34, conducted in dichloromethane at a scan rate of 100 mV/sec. Working electrode: platinum disk, counter electrode: platinum wire, reference electrode: Ag/AgCl. 0.1 M tetrabutylammonium hexafluorophosphate as base electrolyte. Scale equals 2 microamps. FIG 12A illustrates a cyclic voltammogram of solutions of compound, 33, and 2 equivalents of tetrabutylammonium fluoride (1), tetrabutylammonium chloride (2), and tetrabutylammonium dihydrogenphosphate (3) conducted in dichloromethane at a scan rate of 100 mV/sec. Working electrode: platinum disk, counter electrode: platinum wire, reference electrode: Ag/AgCl. 0.1 M tetrabutylammonium hexafluorophosphate as base electrolyte. Scale equals 5 microamps. FIG 12B illustrates a cyclic voltammogram of solutions of compound, 34, and 2 equivalents of tetrabutylammonium fluoride (1), tetrabutylammonium chloride (2), and tetrabutylammonium dihydrogenphosphate (3) conducted in dichloromethane at a scan rate of 1200 mV/sec. Working electrode: platinum disk, counter electrode: platinum wire, reference electrode: Ag/AgCl. 0.1 M tetrabutylammonium hexafluorophosphate as base electrolyte. Scale equals 5 microamps. FIG. 13 illustrates a calix[4]pyrrole-R-(+)-a-methylbenzylamine conjugate, 40, -fluoride complex represented as a diastereomeric mixture. FIG. 14 illustrates the hydrogen bond donating capabilities of a eso-mono- butylamidotrispirocyclohexylcalix[4]pyrrole, 28, complexed to an anion. FIG. 15A illustrates we-fosubstituted-calix[4jpyrrole substituted silica gel, M. FIG. 15B illustrates β-substituted-calix[4jpyrrole substituted silica gel, B. FIG. 16 illustrates the separation of phenyl arsenate (1), benzene sulfonate (2), benzoate (3), and phenyl phosphate (4) on a me_fo-substituted calix[4jpyrrole column: mobile phase, 50 mM phosphate buffer, pH = 7.0: flow rate 0.3 mL/min.; column temperature 25°C; detection 254 nm (0.100 AUFS). FIG. 17 illustrates the separation of 5'-adenosine monophosphate (1), 5'-adenosine diphosphate (2), and 5'-adenosine triphosphate (3) on a weso-substituted calix[4jpyrrole column: mobile phase 250 mM phosphate buffer, pH = 7.0; flow rate 0.3 mL/min.; column temperature 25°C; detection 254 nm (0.100 AUFS). FIG. 18 illustrates the separation of 5'-adenosine monophosphate (1 ), 5'-adenosine diphosphate (2), and 5'-adenosine triphosphate (3) on a β-substituted calixf4jpyrrole column: mobile phase 105 mM phosphate buffer, pH = 7.0; flow rate 0.20 mL/min.; column temperature 25°C; detection UV at 254 nm (0.100 AUFS). FIG. 19 illustrates the separation of uridine (1), phenol (2), aniline (3), benzene (4), and 4-iodo-nitro benzene (5) on a mejo-substituted calixf4jpyrrole column: mobile phase 60% water/ 40% acetonitrile (v/v); flow rate 0.3 mL/min.; column temperature 25°C; detection 254 nm (0.100 AUFS). FIG. 20 illustrates the separation of Cbz-N-protected- serine (1), glutamine (2), alanine (3), phenylalanine (4), tryptophan (5), aspartate (6), and glutamate (7) on a meso-substituted calix[4]pyrrole column: mobile phase 70% 30 mM acetate buffer, pH = 7.0/ 30 % CH3CN (v/v); flow rate 0.3 mL/min.; column temperature 25 * C, detection 254 nm (0.100 AUFS). FIG. 21 illustrates the separation of Cbz-N-protected- serine (1), leucine (2), alanine (3), phenylalanine (4), tryptophan (5), aspartate (6), and glutamate (7) on a β -substituted calix[4]pyrrole column: mobile phase 75/25 30 mM acetate buffer, pH - 7.0/ acetonitrile; flow rate 0.20 mL/min.; column temperature 25 °C; detection UV at 254 nm (0.100 AUFS). FIG. 22 illustrates the separation of 4-fluorobiphenyl (1), 4,4'-difluorobiphenyl (2), 2,2',3,3',5,5',6,6',-octafluorobiphenyl (3), and perfluorobiphenyl (4) on a meso-substituted calix[4jpyrrole column: mobile 60% water/40% acetonitrile (v/v); flow rate 0.3 mL/min.; column temperature 25°C; detection 254 nm (0.100 AUFS). FIG. 23 illustrates the separation of 4-fluorobiphenyl (1), 4,4'-difluorobiphenyl (2), 2,2',3,3',5,5',6,6',-octafluorobiphenyl (3), and perfluorobiphenyl (4), on a β-substituted calix[4]pyrrole column: mobile 76% water/24% acetonitrile (v/v); flow rate 0.2 mL/min.; column temperature 25°C; detection 254 nm (0.100 AUFS). FIG. 24 illustrates the separation of a mixture of polydeoxythymidilates oligomers containing between (12) and (18) base pairs on a rne-yo-substituted calix[4jpyrrole column: mobile phase 50/50 acetonitrile/ 50 mM phosphate, 250 mM chloride buffer, pH = 6.8 (v/v); flow rate 0.4 mL/min.; column temperature 25 °C; detection UV at 265 nm. FIG. 25 illustrates the separation of a mixture of polydeoxythymidilates oligomers containing between (12) and (18) base pairs on a β-substituted calixf4jpyrrole column: mobile phase 40/60 acetonitrile/ 40 mM phosphate, 50 mM chloride buffer, pH = 7.0 (v/v), flow rate 0.25 mL/min.; column temperature 25'C; detection UV at 262 nm (0.100 AUFS). FIG. 26 illustrates the separation of TCTAGA, (1), GCATGC, (2), and CCCGGG, (3), on a meso-substituted calix[4jpyrrole column: mobile phase 50/50 acetonitrile/ 50 mM sodium phosphate, pH = 7.0 (v/v); flow rate 0.4 mL/min.; column temperature 25"C; detection UV at 265 nm (0.100 AUFS). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention provides β-substitued calixfnjpyrroles, calixfmjpyridines, calixfnjpyrrole-anion complexes, calixfmjpyridinofnjpyrrole-anion complexes, calixfnjpyrrole- neutral molecule complexes, calixfmjpyridinofnjpyrrole-neutral molecule complex, calixfmjpyridinofnjpyrrole-cation complexes, calixfmjpyridine-cation complexes, calixfmjpyridine-neutral molecule complexes and certain m so-substituted calix[4jpyrroles, mesø-substituted calixfnjpyrroles where n is 5, 6, 7, or 8, certain calixfmjpyridinofnjpyrroles, where m + n = 4, and calixfmjpyridinofnjpyrroles where m + n = 5, 6, 7, or 8 as new compositions of matter. Specific anions bound by macrocycles of the present invention include, but are not limited to, halide anions, carboxylates, phosphates, sulfates, oxalates, terephthalates, phospholipids, nucleotides, oligonucleotides, DNA, RNA, anionic polyoxometalates, or oxoanions such as pertechnetate as demonstrated in the examples herein, size and charge of the anion appear not to be limiting factors in the type of anion that may be bound since oligonucleotides are able to be separated on calixf4jpyrrole modified HPLC columns. The term "molecular species" as used herein, means a neutral molecule, and represents a variety of classes of molecules since the macrocycles of the present invention provide different sizes of cavities and varieties of donor and acceptor sites. Specific neutral molecules include, but are not limited to, alcohols, polyalcohols, ketones, polyketones, phenols, polyhydroxylated aliphatic and aromatic compounds, amino compounds, amino acids, urea, guanidine, saccharides, biologically important polymers like proteins derivatives, and the like. Additionally, neutral molecules include, but are not limited to, ion pairs such as NaCl, Csl, or any grouping of ions which is neutral overall, or zwitterionic species such as amino acids and the like. Specific cations include, but are not limited to, Group 1 metals, Group 2 metals, transition metals, post-transition metals, lanthanides, actinides, ammonium, alkylammonium, arylammonium, hydroxonium and guanidinium. Carbon atoms in pyrrole rings are referred to as α or β; α carbons are adjacent to the NH group and β-carbons are adjacent to the α-carbons (see IV). In an alternative designation, the nitrogen atom is labelled 1 and the other atoms are numbered sequentially starting from an adjacent carbon atom. Thus, the α-carbons are also referred to as atoms 2 and 5 and the β- carbons as atoms 3 and 4. 4 IV Similarly, carbon atoms in pyridine rings are referred to as α, β or γ; α carbons are adjacent to the N atom, β-carbons are adjacent to the α-carbons and the γ-carbon is adjacent to the two β-carbons (see V). In an alternative designation, the nitrogen atom is labelled 1 and the other atoms are numbered sequentially starting from an adjacent carbon atom. Thus, α-carbons are also referred to as atoms 2 and 6, β-carbons as atoms 3 and 5, and the γ-carbon as atom 4. As used herein, "a non-α, on-meso, macrocyclic carbon" means a β or a γ carbon; and "macrocyclic nitrogen" means a pyrrole or pyridine nitrogen. Substituents for Macrocycles ofthe Present Invention Representative examples of alkanes useful as alkyl group substituents of the present invention include straight-chain, branched or cyclic isomers of propane, butane, pentane, hexane, heptane, octane, nonane, and decane, with methane, ethane, propane, and cyclohexane being preferred. Alkyl groups having up to about thirty, or up to about fifty carbon atoms are contemplated in the present invention. Representative examples of substituted alkyls include alkyls substituted by two or more functional groups as described herein. Representative examples of alkenes useful as alkenyl group substituents include ethene, straight-chain, branched or cyclic isomers of propene, butene, pentene, hexene, heptene, octene, nonene, and decene, with ethene and propene being preferred. Alkenyl groups having up to about thirty or fifty carbon atoms, and up to about five double bonds, or more preferably, up to about three double bonds are contemplated in the present invention. Representative examples of alkynes useful as alkynyl group substituents include ethyne, straight-chain, branched or cyclic isomers of propyne, butyne, pentyne, hexyne, heptyne, octyne, nonyne, and decyne, with ethyne and propyne being preferred. Alkynyl groups having up to about thirty, or up to about fifty carbon atoms, and having up to about five or up to about three triple bonds are contemplated in the present invention. The aryl may be a compound whose molecules have the ring structure characteristic of benzene, naphthalene, phenanthrene, anthracene, and the like, i.e., either the 6-carbon ring of benzene or the condensed 6-carbon rings of the other aromatic derivatives, or a compound whose molecules have the ring structure characteristic of pyridine i.e., a 5-carbon-l -heteroatom ring, or pyπole i.e., 4-carbon- 1 -heteroatom ring such as, but not limited to, pyrrole, furan, or thiophene or the condensed 5-or 6-atom rings ofthe other aromatic derivatives. For example, an aryl group may be phenyl or naphthyl, and the terms used herein include both unsubstituted aryls and aryls substituted with one or more nitro, carboxy, sulfonic acid, hydroxy, oxyalkyl or halide substituent. Purine or pyrimidine molecules are included as "aryl" molecules. In this case, the substituent on the phenyl or naphthyl may be added in a synthetic step after the condensation step which forms the macrocycle. Among the halide substituents, chloride, bromide, fluoride and iodide are contemplated in the practice of this invention. Representative examples of haloalkyls used in this invention include halides of methane, ethane, propane, butane, pentane, hexane, heptane, octane, nonane and decane, with halides, preferably chlorides or bromides, of methane, ethane and propane being preferred. Representative examples of haloalkenyls used in this invention include halides of methene, ethene, propene, butene, pentene, hexene, heptene, octene, nonene and decene, with halides, preferably chlorides or bromides, of methene, ethene and propene being preferred. Representative examples of haloalkynyls used in this invention include halides of methyne, ethyne, propyne, butyne, pentyne, hexyne, heptyne, octyne, nonyne and decyne, with halides, preferably chlorides or bromides, of methyne, ethyne and propyne being preferred. "Hydroxyalkyl" means alcohols of alkyl groups. Prefeπed are hydroxyalkyl groups having one to twenty, more preferably one to ten, hydroxyls. "Hydroxyalkyl" is meant to include glycols and polyglycols; diols of alkyls, with diols of Cl-10 alkyls being preferred, and diols of C 1-3 alkyls being more preferred; and polyethylene glycol, polypropylene glycol and polybutylene glycol as well as poiyalkylene glycols containing combinations of ethylene, propylene and butylene. Representative examples of alkoxys include the alkyl groups as herein described having ether linkages. "Alkoxy" is meant to include polyethers with one or more functional groups. The number of repeating alkoxys within a substituent may be up to 200, preferably is from 1- 20, and more preferably, is 1-10, and most preferably is 1-5. A preferred alkoxy is O(CH2CH2O) x CH3 where x = 1-100, preferably 1-10, and more preferably, 1-5. "Crown ether" means a cyclic polyether with repeating alkoxy groups. They are named according to the number of atoms and the number of oxygens in the cycle. S-crown-T is a cyclic polyether containing S atoms and T oxygen atoms. Preferred are crown ethers containing one to twenty oxygen atoms, more preferably one to ten. Representative examples include, but are not limited to, 12-crown-4, 15-crown-5, benzo-15-crown-5, 18-crown-6, benzo-18-crown-6, 21- crown-7, 24-crown-8, dibenzo-18-crown-6 and functionalized derivatives thereof. "Hydroxyalkenyl" means alcohols of alkene groups. Preferred are hydroxyalkenyl groups having one to twenty, more preferably one to ten, hydroxyls. "Hydroxyalkynyl" means alcohols of alkyne groups. Preferred are hydroxyalkenyl groups having one to twenty, more preferably one to ten, hydroxyls. "Hydroxyalkoxy" means alkyl groups having ether or ester linkages, hydroxyl groups, substituted hydroxyl groups, carboxyl groups, substituted carboxyl groups or the like. Representative examples of thioalkyls include thiols of ethane, thiols of straight-chain, branched or cyclic isomers of propane, butane, pentane, hexane, heptane, octane, nonane and decane, with thiols of ethane (ethanethiol, C2H5SH) or propane (propanethiol, C3H7SH) being preferred. Sulfate-substituted alkyls include alkyls as described above substituted by one or more sulfate groups, a representative example of which is diethyl sulfate ((C2H5)2SO4). "Alkylsulfoxide" means alkyl groups having S=O groups. Preferred are alkylsulfoxide groups having one to twenty, more preferably one to ten, sulfoxides. Alkylsulfoxide is meant to include cyclic alkyl groups containing sulfoxide moieties. "Alkylsulfone" means alkyl groups having S(=O)2 groups. Preferred are alkylsulfone groups having one to twenty, more preferably one to ten, sulfones. Alkylsulfone is meant to include cyclic alkyl groups containing sulfone moieties. "Alkylsulfide" means alkyl groups having S groups. Preferred are alkylsulfide groups having one to twenty, more preferably one to ten, sulfides. Alkylsulfide is meant to include cyclic alkyl groups containing sulfide moieties such as tetrahydrothiopyran derivatives. Representative examples of phosphates include phosphate groups, polyphosphate groups, DNA, RNA, oligonucleotides and nucleotides. Representative examples of phosphate- substituted alkyls include alkyls as described above substituted by one or more phosphate or polyphosphate groups. Representative examples of phosphonate-substituted alkyls include alkyls as described above substituted by one or more phosphonate groups. Representative examples of carboxy groups include carboxylic acids of the alkyls described above as well as aryl carboxylic acids such as benzoic acid and derivatives thereof. Representative examples of carboxyamides include peptides, proteins, primary carboxyamides (CONH2), secondary (CONHR') and tertiary (CONR'R") carboxyamides where each of R' and R" is a functional group as described herein. Representative examples of useful amines include a primary, secondary or tertiary amine of an alkyl or aryl as described herein. "Carboxyamidealkyl" means alkyl groups with secondary or tertiary amide linkages or the like. "Carboxyalkyl" means alkyl groups having hydroxyl groups, carboxyl or amide substituted ethers, ester linkages, tertiary amide linkages removed from the ether or the like. The term "saccharide" includes oxidized, reduced or substituted saccharide; hexoses such as D- or L-glucose, D- or L-mannose or D- or L-galactose; pentoses such as D- or L-ribose or D- or L-arabinose; ketoses such as D- or L-ribulose or D- or L-fructose; disaccharides such as sucrose, lactose, or maltose; derivatives such as acetals, amines, and phosphorylated sugars; oligosaccharides or polysaccharides, such as aliginic acid as well as open chain forms of various sugars, and the like. Examples of amine-derivatized sugars are galactosamine, glucosamine, sialic acid and D- and L-glucamine derivatives such as 1 -amino- 1-deoxysorbitol. By "reporter group" is meant a substituent that is fluorescent, chromophoric, electropolymerizable, redox-active, or optically active. Examples of a fluorescent reporter group include, but are not limited to, ruthenium (II) bipyridyl complexes, acetylnaphthalene, 9- aminoacridine, 9-phenylanthracene, benzimidazole, N-methylbenzo[b]carbazoIe, 2- phenylbenzoxazole, l,l '-binaphthyl, fluorene, fluorescein dianion, indenof2,l-ajindene, 2,5- diphenylfuran, perylene, 2-aminopurine, 7-quatephenyl, 4,4'-diphenylstilbene, sapphyrins and texaphyrins. Examples of a chromophoric reporter groups include isosulfan blue, fluoroscein, 2',7'-dichiorofluoroscein, rhodamine, carboxyrhodamine, dialkylaminocoumarin, erythrosin, pyrene, 9-(diethylamino)-5-octadecanoylimino-5H-benzo[ajphenoxazine, 5-octadecanoyloxy-2- (4-nitrophenylazo)phenol, 9-(diethylamino)-5-(2-naphtoylimino)-5H-benzofajphenoxazine, 4',5'-dibromoflurescein octadecyl ester, 2-(4-nitrophenylazo)chromotropic acid disodium salt, 2-(phenylazo)chromotropic acid disodium salt , 4,5-dihydroxynaphtalene-2,7-disulfonic acid disodium salt, , 5,7-dihydroxyflavone, 5,7-dinitro-8-hydroxy-2-naphthalenesulphonic acid, 6,6'- f(3,3'-dimethyl[l, -biphenylj4,4'-diyl)bis(azo)jbis[4-amino-5-hydroxy-l,3- naphthalenedisulfonic acidj tetrasodium salt, 4,5,6, 7,-tetrachloro-3', 6'-dihydroxy-2',4', 5',7'- tetraiodospirofisobenzofuran-l (3H),9'-[9Hjxanthenj-3-one dipotassium salt, 3,7- bis(dimethylamino)phenothiazin-5-ium chloride, 3,6-bis(dimethylamino)acridine hydrochloride zinc chloride double salt (Acridine Orange), sapphyrins and texaphyrins. By "electropolymerizable" is meant a moiety that will polymerize when subjected to a particular voltammetric potential or a continuous scanning potential. Examples of an electropolymerizable group include, but are not limited to, α-free pyrroles, α-free thiophenes, anilines or vinyl groups. By "redox-active" is meant a moiety which can undergo an oxidation or a reduction process. Examples of a redox-active group include, but are not limited to, ferrocene, cobaltocenium, ruthenium (II) bipyridyl complexes, transition metals, fullerenes, poφhyrins, expanded poφhyrins and pyrroles. In one embodiment of the present invention, a calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine is further coupled to a site-directing molecule. "Site-directing" means having specificity for targeted sites. "Specificity for targeted sites" means that upon contacting the calixfnjpyrrole-, calixfmjpyridinofnjpyrrole- or calixfmjpyridine-conjugate with the targeted site, for example, under physiological conditions of ionic strength, temperature, pH and the like, specific binding will occur. The interaction may occur due to specific electrostatic, hydrophobic, or other interaction of certain residues of the conjugate with specific residues ofthe target to form a stable complex under conditions effective to promote the interaction. Exemplary site-directing molecules contemplated in the present invention include but are not limited to: polydeoxyribonucleotides; polyribonucleotides; oligodeoxyribonucleotides; oligoribonucleotides; polyamides, including peptides having affinity for a biological receptor, and proteins such as antibodies; steroids and steroid derivatives; hormones such as estradiol, or histamine; hormone mimics such as moφhine; and further macrocycles such as texaphyrins, sapphyrins or rubyrins. Representative examples of useful steroids include a steroid hormone of the following five categories: progestins (e.g. progesterone), glucocorticoids (e.g., cortisol), mineralocorticoids (e.g., aldosterone), androgens (e.g., testosterone) and estrogens (e.g., estradiol). The term "a peptide having affinity for a biological receptor" means that upon contacting the peptide with the biological receptor, for example, under appropriate conditions of ionic strength, temperature, pH and the like, specific binding will occur. The interaction may occur due to specific electrostatic, hydrophobic, entropic or other interaction of certain amino acid or glycolytic residues of the peptide with specific amino acid or glycolytic residues of the receptor to form a stable complex under the conditions effective to promote the interaction. The interaction may alter the three-dimensional conformation and the function or activity of either or both the peptide and the receptor involved in the interaction. A peptide having affinity for a biological receptor may include an endoφhin, an enkephalin, a growth factor, e.g. epidermal growth factor, poly-L-lysine, a hormone, a peptide region of a protein and the like. A hormone may be estradiol, for example. Representative examples of useful amino acids of peptides or polypeptides include amino acids with simple aliphatic side chains (e.g., glycine, alanine, valine, leucine, and isoleucine), amino acids with aromatic side chains (e.g., phenylalanine, tryptophan, tyrosine, and histidine), amino acids with oxygen-, and sulfur-containing side chains (e.g., serine, threonine, methionine, and cysteine), amino acids with side chains containing carboxylic acid or amide groups (e.g., aspartic acid, glutamic acid, asparagine, and glutamine), and amino acids with side chains containing strongly basic groups (e.g., lysine and arginine), and proline. Representative examples of useful peptides include any naturally occurring or synthetic di-, tri-, tetra-, pentapeptides or longer peptides derived from any of the above described amino acids (e.g., endorphin, enkephalin, epidermal growth factor, poly-L-lysine, or a hormone). Representative examples of useful polypeptides include both naturally occurring and synthetic polypeptides (e.g., insulin, ribonuclease, and endoφhins) derived from the above described amino acids and peptides. The term "binding agent" means that upon contacting the binding agent with a guest species for binding under appropriate conditions of ionic strength, temperature, pH and the like, specific binding will occur. The interaction may alter the three-dimensional conformation and the function or activity of either or both the bound species and the receptor involved in the interaction. Preferred binding agents include a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, a calixfmjpyridine, a calixarene, a cation-binding functionality, a crown ether, a chelating group, a porphyrin, or an expanded porphyrin such as sapphyrin, rosarin, rubyrin, texaphyrin, amethyrin or turcasarin, or oligopyrroles. Texaphyrin compounds, methods for making and methods for using them are described in U.S. Patents 4,935,498, 5,162,509, 5,252,720, 5,272,142, 5,256,399, 5,292,414, 5,432,171, 5,439,570, 5,475,104, 5,451,576, 5,457,183, 5,369,101, 5,569,759, 5,559,207, 5,587,463, 5,594,136, and 5,599,923; in pending application USSN 08/484,551; and in PCT publications WO 90/10633, WO 93/14093, WO 94/29316, and WO 96/38461; each patent, application, and publication is incoφorated by reference herein. Rubyrins are disclosed in U.S. Patent 5,410,045, and turcasarins are disclosed in PCT publication WO 96/21665; the patent and PCT publication are incoφorated by reference herein. Sapphyrins are disclosed in U.S. Patents 5,041,078; 5,159,065; 5.120,41 1 ; 5,302,714; 5,457,195; 5,530,123; 5,543,514; and 5,587,478; each patent is incoφorated by reference herein. A "catalytic group," as used herein, means a chemical functional group that may act as a general acid, Brønsted acid, Lewis acid, general base, Brønsted base, Lewis base, nucleophile, or any other means by which an activation barrier to reaction is lowered or the ground state energy of a substrate is increased. Exemplary catalytic groups contemplated include, but are not limited to, imidazole; guanidine; zinc coordinated to a nitrogen containing macrocycle, EDTA complexes, DTPA compleses, substituted saccharides such as a D-glucosamine, D- mannosamine, D-galactosamine, D-glucamine, and the like; amino acids such as L-histidine and L-arginine; derivatives of amino acids such as histamine; polymers of amino acids such as poly- L-lysine, (LysAla) n , (LysLeuAla) n where n is from 1-30 or preferably 1-10 or more preferably 2-7 and the like. The catalytic group may be attached either directly to the calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine or via a linker or couple of variable length. A couple may be described as a linker, i.e. the covalent product formed by reaction of a reactive group designed to attach covalently another molecule at a distance from the calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine macrocycle. Exemplary linkers or couples are amides, amine, disulfide, thioether, ether, ester, or phosphate covalent bonds. In most preferred embodiments, conjugates and appended groups are covalently bonded to the calixfnjpyrrole via a carbon-carbon, carbon-nitrogen, carbon-sulfur, or a carbon-oxygen bond, more preferably a carbon-carbon, carbon-oxygen or a carbon-nitrogen bond. Preferred embodiments of the present invention include a calixfnjpyrrole where n is 4 - 8, calixfmjpyrindinofnjpyrrole where m+n is 4 - 8; other than when each of m and n is 2, and other than when m is 1 and n is 3; or calixfmjpyridine where n is 4 - 8; where at least one substituent attached to a meso-carbon is carboxy or carboxyalkyl, or at least one subsituent attached to a β- or γ- carbon is other than hydrogen, or at least one substituent attached to a β- or γ- carbon is carboxy, carboxyalkyl, ester or carboxyamide. Further preferred embodiments of the present invention include a calixfnjpyrrole; a calixfmjpyridinofnjpyrrole where n is 4 - 8 other than when each of m and n is 2, and other than when m is 1 and n is 3; or calixfmjpyridine where m is 4 - 8; where at least one of the nitrogen atoms is bound to hydrogen. A calixf4jpyrrole where all pyrrole nitrogen atoms are bound to hydrogen, where meso-carbons are bound to methyl, and one β-substituent is (CH2)tCOOH where O≤t≤lO. Another preferred calixf4]pyπole has each of three meso-carbons bridged to itself in a spirocyclohexyl substituent, one mesø-carbon is bonded to a methyl group and to a -CH2CH2CH2COOH group, β-subsituents are hydrogen and pyrrolic nitrogens are bound to hydrogen. Most preferred macrocycles of the present invention are as follows. Compound # Maςroςyςle 3 mesø-monoester-mono-methyl-tri-spirocyclohexylcalix[4]pyrrole 4 me-fo-monoacid-mono-methyl-tri-spirocyclohexylcalixf4jpyrrole 5 meso-tetravinyltetramethylcaIix[4jpyrrole 6 wefooctabenzylcalixf4jpyrrole 7 mesø-tetraspirotetrahydrothiopyrancalix[4]pyrrole 8 7Mejø-octanonylcalixf4jpyrrole 9 w&so-tetramethyl-me£ø-tetraferrocenylcalix[4]pyπOle 10 β-monoester-meyo-octamethy lcalix[4 jpyrrole 11 β-diester-/we.so-octamethylcalix[4 jpyrrole 12 β-monoacid- e5ø-octamethylcalixf4]pyrrole 13 β-octamethoxy-wwø-tetraspirocyclohexylcalix[4 jpyrrole 14 β-octabromo-meso-octamethylcalix[4]pyrrole 15 /Me5o-p-rert-butyl-calixarene-tetramethylcalix[4]pyrrole pseudo dimer 16 calixarene-tetramethylcalix[4jpyrrole-calixarene pseudo trimer 17 me.ϊø-decamethylcalix[5]pyrrole 18 mesø-dodecamethylcalix[6]pyrrole 19 me5ø-tetradecamethylcalix[7]pyrrole 20 meso-hexadecamethylcalixf8]pyrrole 22 β-octaethyl- e5O-octamethylcalix[4jpyrrole 26 merø-octamethyl-calix[3 jpyridinof 1 jpyrrole 27 me5o-octamethylcalix[4jpyridine 28 mono-β-butylamidocalix[4]pyrrole 29 mono- mes o-buty lamido-heptamethylcalix [4]pyrrole 30 mono-β-octadecyl-me.sø-octamethylamidocalix[4Jpyrrole 31 propyl bridged β-amido-/we5ø-octamethylcalix[4]pyrrole dimer 32 β-amidobenzo- 15-crown-5-mesø-octamethylcalix[4]pyrrole 33 Mono-mesø-feπoceneamido-mono-methyl-tri- spirocyclohexylcalix[4]pyrrole 34 Mono-β-ferroceneamido-me5ø-octamethylcalixf4jpyrrole 35 mesø-tetrakispolyethyleneglycol-mesø-tetramethyl substituted calixf4jpyrrole 36 2-amido-2-deoxy-l,3,4,6-tetra-O-acetyl-β-D- glucopyranosecaiix[4]pyrrole conjugate (chiral) 37 2-amido-2-deoxy-β-D-glucopyranosecalix[4]pyrrole conjugate (chiral) 38 me.so-tetraspiro-4-cholesten-3-onylcalix[4jpyrrole (chiral) 39 mesø-tetrabinaph al-meso-tetramethylcalixf4]pyrrole (chiral) 40 β-(R)-(+)-α-methylbenzylamidocalix[4jpyrrole (chiral) 41 me.sø-./V-protected-guanine-7wesø- trispirocy clohexy lcalix[4 jpyrrole conj ugate 42 me^o-Λ^-protected-cytosine-ffieΛO- trispirocyclohexylcalix[4]pyrrole conjugate Most preferred are macrocycles 3, 4, 10, 12 and solid supported materials Gel M and Gel B. While the cited calixfnjpyrroles, calixfmjpyridinofnjpyrroles or calixfmjpyridines are presently preferred for use in the present invention, the invention is not limited thereto and any calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine may be used. Synthetic Methods for Macrocycles ofthe Present Invention The present disclosure provides a number of different methods for making macrocycles ofthe present invention. The following preferred embodiments are described. Calixfnjpyrroles having an ester functionality at a meso-carbon. A method of making a calixfnjpyrrole having an ester functionality at a meso-carbon comprises reacting a ketone having one or more ester functionalities with a pyrrole in the presence of a Brønsted acid. The method of invention additionally comprises reacting a mixture of ketones containing one or more ester functionalities and ketones not containing ester functionalities with a Brønsted acid to provide a range of ester functionalities. Examples of ketones having one or more ester functionalities include, but are not limited to, methylacetylbutyrate, acetylpropionate and acetylpentanoate. Examples of pyrroles include, but are not limited to, pyrrole, 3,4- diethylpyrrole, 3, 4-dimethoxy pyrrole or 3-butylpyrrole. Examples of Brønsted acids include, but are not limited to HCl, methanesulfonic acid, toluenesulfonic acid, benzenesulfonic acid, HClθ4 ( , formic acid, acetic acid, sulfuric acid or phosphoric acid. As demonstrated in Example 2, methylacetylbutyrate was reacted with pyrrole and cyclohexanone in the presence of a Brønsted acid to form a macrocyclic structure comprised of n pyrrole units, where n is 4, 5, 6, 7, or 8 and the number of ester functionalities is equal to 1 to n. Calixfnjpyrroles having an acid functionality at a meso-carbon. This method embodiment for making calixfnjpyrroles having an acid functionalities at a meso-carbon comprises de-esterifying an ester-functionalized calixfnjpyrrole where n is 4, 5, 6, 7 or 8. De- esterifying may be accomplished by heating the ester-functionalized calixfnjpyrrole in a water/ethanol solution in the presence of sodium hydroxide and subsequent acidification and extraction ofthe calixpyrrole acid. Addition of an electrophile to a calixfnjpyrrole to form a β-substituted calixfnjpyrrole. This method embodiment comprises activating a calixfnjpyrrole, where n is 4, 5, 6, 7 or 8 and reacting the activated calixfnjpyrrole with an electrophile to make a β-substituted calixfnjpyrrole. Examples of activation include, but are not limited to, deprotonation with a Brønsted base such as n-butyl lithium, /er/-butyl lithium, or phenyl lithium, or a Brønsted base in conjunction with a complexing agent such as tetramethylethylenediamine. Examples of electrophiles include, but are not limited to, ethylbromoacetate, methylbromoacetate, acrylonitrile, methyl acrylate, acyl chloride, alkyl halide, functionalized alkyl halides or carbon dioxide. An exemplary method is found in Example 3. β-Subtituted calixfnjpyrroles. A method of making a β-subtituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprises reacting a β-substituted pyrrole with a ketone in the presence of a Brønsted acid to produce a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7 or 8. Examples of β-substituted pyrroles include, but are not limited to, 3,4-dimethoxypyrrole, 3,4-diethyl pyrrole 3,4-dimethylpyrroIe, 3,4 diphenylpyrrole, or 3-butylpyrrole. Examples of ketones include, but are not limited to, acetone, pentan-3-one, dibenzyl acetone, 5-nonone, 4-heptanone, cyclohexanone, or cyclopentanone. Examples of Brønsted acids have been described herein. A method of making a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising producing a calixfnjpyrrole radical where n is 4, 5, 6, 7, or 8; and reacting the radical with a radical acceptor molecule such as iodine, methyl acrylate or iodoacetamide. An exemplary method is described in Example 3. Lewis acid condensation. A pyrrole and a ketone are reacted in the presence of a Lewis acid to form a calixfnjpyrrole where n is 5, 6, 7, or 8. Examples of ketones and pyrroles have been described herein. Examples of Lewis acids include, but are not limited to, electron pair acceptors containing empty orbitals such as aluminum trichloride, boron trifluoride, gallium trichloride, or high valent metals such as titanium (IV), vanadium (V), tin (IV), manganese (III), or Uθ2 2+ . Exemplary methods include the methods described in Example 5. Heterogeneous condensation. A synthetic method for heterogeneous condensation to form calixfnjpyrroles where n is 4, 5, 6, 7 or 8 comprises reacting a pyrrole and a ketone in the presence of a heterogeneous catalyst. Examples of pyrroles and ketone have been described herein. Examples of heterogeneous catalysts include, but are not limited to, montmorrilonite, zeolite, silica gel, alumina, or a cation-exchange solid support. Exemplary methods include those methods found in Example 5. Calixfnjpyrroles from pyrrole alcohols. A method of synthesis of calixfnjpyrroles using pyrrole alcohols comprises reacting a ketone-functionalized pyrrole with an alkyl metal or aryl metal to form a pyrrole alcohol; condensing the pyrrole alcohol with a Lewis acid, a Brønsted acid or a heterogeneous catalyst to form a cyclizable pyrrole oligomer; and repeating the condensing n - 2 times to form a calixfnjpyrrole. Examples of pyrrole ketones include, but are not limited to, 2-acetyl-3,4-diethylpyrrole, 2-acetylpyrrole, 2-acetyl-3,4-dimethylpyrrole, 2-alkylacetyl-3,4-disubstituted pyrrole, 2-alkylacetyl-3-substituted pyrrole, or 2-alkylacetyl-4- substituted pyrrole. Examples of alkyl metals include, but are not limited to, alkyllithium, Grignard reagent, organocuprates, organozincs or organotin compounds. Examples of aryl metals include, but are not limited to, phenyllithium. Using 2-acetylpyrrole and an alkyl lithium to illustrate the method described herein, 2- acetylpyrrole is reacted with butyl lithium to produce 1 -butyl- 1 -methyl- l-(2-pyrrolo) methanol. 1 -Butyl- 1 -methyl- l-(2-pyrrolo) methanol is condensed with 2-acetylpyrrole in the presence of a Brønsted acid. The reaction is repeated twice to form a calixf4 jpyrrole. Template synthesis. This synthetic method embodiment comprises reacting a ketone and pyrrole in the presence of a Lewis acid, a Brønsted acid or a heterogeneous catalyst in the presence of a template. Examples of pyrroles, ketones, Lewis acids, Brønsted acids, and heterogeneous catalysts have been given herein. Examples of templates include, but are not limited to, tetraphenylporphyrin, hydrogen bonding neutral molecules, such as sugars, cyclodextrins, alcohols or amines or anionic species such as, halides, carboxylates, sulfonates or phosphates. Using halides to illustrate, but not limit, the methods of template synthesis of calixfnjpyrroles, pyrrole and acetone would be condensed using a Brønsted acid, a Lewis acid or a homogeneous catalyst in the presence of a halide. By varying the size ofthe halide, it would be possible to "tune" the size of calixfnjpyrrole where n is 4, 5, 6, 7, or 8. Pyrrole Ring Expansion. This synthetic method embodiment comprises reacting a calixpyrrole or calixpyridinopyrrole with a halocarbene in a cycloaddition reaction, converting a pyrrole to a pyridine and forming a calixpyridinopyrrole or a calixpyridine. Exemplary procedures for this cycloaddition are found herein in Example 6. Dihalocarbenes can also be generated from chloroform or bromoform in the presence of a strong base (e.g. /er/-BuOK) or under phase transfer catalysis conditions (e.g. in the presence of triethylbenzylamrnonium bromide) in chloroform or bromoform with a concentrated sodium hydroxide solution (50%). Calixfnjpyrroles having different substituents at meso-carbons. A method of synthesizing a calixfnjpyrrole where n is 5, 6, 7, or 8 having different substituents at a meso- carbon comprises reacting at least two ketones with a pyrrole in the presence of a Lewis acid. Examplary ketones and Lewis acids are described herein. One skilled in the art of organic synthesis, in light of the present disclosure, could extend and refine the referenced basic synthetic chemistry to produce calixfnjpyrroles having various substituents. For example, polyether-linked polyhydroxylated groups, saccharide substitutions in which the saccharide is appended via an acetal-like glycosidic linkage, an oligosaccharide or a polysaccharide may be similarly linked to a calixfnjpyrrole. A carboxylated calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine in which the acid groups are linked to the calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine core via aryl ethers or functionalized alkyl substituents could be converted to various amido products wherein the amide linkages serve to append further substituents. Saccharide moieties may be appended via amide bonds. Calixarene-calixpyrrole pseudo dimers, multimers and polymers Calixfnjarenes are macrocyclic molecules composed of phenol rings linked by methylene groups. According to one aspect of the present invention, calixfnjpyrroles are useful as anion binding agents. A method of the invention comprises reacting a cyclic ketone-containing compound with pyrrole. The number of ketones and the number of pyrroles will be equal to n. The cyclic ketone-containing compound acts as a template upon which the calixpyrrole is formed, after which the synthesized calixpyrrole may be detached from the ketone-containing template. Alternatively, where the template is a calixarene, the resulting cylindrical calixfnjarene-calixfnjpyrrole pseudo dimer may be kept intact. The cyclic ketone-containing compound may be selected from any cyclophane molecule having n ketone groups. Examples of such molecules include, but are not limited to, calixfnjarenes, resorcinarenes, CTV, and cyclic sugars such as the cyclodextrins. Alternatively, benzene rings substituted with ketone groups, or azacrown ethers with N-pendant ketone moieties may serve as templates in the synthesis of the present invention. In one aspect of the invention, it is preferred that the ketone groups of the template all be pointing in the same direction. For example, in the case of calixarenes, the calixarene is in a cone conformation. In order to detach a templated calixfnjpyrrole, a cleavable group is present between the template and the ketone. This cleavable group may be covalently linked or non-covalently linked. The cleavage group may be, for example, an ester that can be subsequently hydrolyzed, or a benzyl group that can be hydrogenated or removed with a de-benzylating agent such as Mβ Sil. Non-covalent interactions may also be employed, such as carboxylic acid dimerization or metal complexation between the template and the ketone. In the synthesis, the pyrrole condensation must be acid catalysed; the acid may be selected from a Brønsted acid, such as methanesulfonic acid, HCl, HCIO4, H2SO4, HNO3, TFA, HCO 2 H, H3PO4; a Lewis acid such as VOCI3, Mn(OAc)3, SnC.4, AICI3, or UO2(OAc)2; and a clay such as montmorrilonite. The synthesis is carried out in an organic solvent that is compatible with the particular ketone-containing template utilized. Solvents such as chloroform, dichloromethane, acetonitrile, carbontetrachloride, toluene, benzene, and mixtures of these solvents with methanol, ethanol, propanol, and the like may be used. The linker to the ketone could also be changed to include any spacer such as benzyl groups, alkynes, alkenes, ethers, polyethers, thioethers, bipyridyl, phenanthroline, anthracene, naphthalene, any polyaromatic, quinone, ferrocene, cobaltocenium or other metallocene, and the like. Using calixarenes to illustrate the method of the present invention, the calix[4]arene skeleton is used as a template, around which a calixf 4 jpyrrole forms. Although calixarene- linked and calixarene-capped poφhyrins as well as calixarenes linked to pyrrole groups are known, to the best of the present inventors knowledge, compound 15 represents the first example of a calixarene-capped calixpyrrole . Calixfnjpyrroles derivatives, multimers, and conjugates. Example 8 provides for the synthesis of different and exemplary derivatives, multimers or conjugates of calixfnjpyrroles. With use of either the meso-"monohook" acid 4 or the β- "monohook" acid 12 there exist possibilities of substitution through various functional groups. Formation of an amide bond, for example, could lead to attachment of natural nucleotides as well as the "unnatural" nucleotide analogues. Using nucleotide-functionalized calixpyrroles, it may be possible to do through-membrane transport of nucleotide analog drugs such as AZT, acyclovir, and the like (Example 11). Using the case of calix[4]pyrrole by way of illustration, one skilled in the art in light of the present invention would appreciate that in addition to one "hook", one may add two, three, four, five, six, seven, or eight "hooks" at the meso- or β- positions of the calixpyrrole. Arrays of calixpyrroles spanning membranes and acting as anion channels analogous to cation channels are contemplated by the present inventors. By using poly-amine-containing compounds, dimers, trimers and tetramers of calixpyrrole could be used as carriers for transporting polyanionic species through aliphatic membranes. Through NMR work and work with HPLC, it has been determined that calixf4jpyrroles show a higher affinity for phosphate anions than other oxyanions. With this in mind, arrays of calixpyrroles could be used to encircle RNA and DNA molecules, thus providing anti-viral activity. Specificity of binding could be gained by attaching specific nucleotides to the calixpyrrole oligomer. Attachment of known nucleotide cleaving agents such as Fe-EDTA could facilitate the site directed cleavage of RNA. Calixf4]pyrrole also binds to carboxylic acid groups by HPLC and could be used for extraction of pesticide and herbicide metabolites. Additionally, with the addition of a cation-binding functionality, amino acid recognition and transport are possible. A solid silica-supported calix[4jpyrrole has shown selectivity of various N-benzyl protected amino acids. This could lead to specific binding of proteins and interuption of enzymatic pathways. The attachment of sugars, and mono- and polysaccharides to the calixpyrrole could allow for recognition of compounds such as glucose-6-phosphate. By attachment of a redox-active reporter group; calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine macrocycles may function as potentiometric or amperometric sensors for cations, anions, or neutral molecules. By attachment of a fluorophoric reporter group; calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine macrocycles may function as fluorescent sensors for cations, anions, or neutral molecules. By attachment of a chromophoric reporter group; calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine macrocycles may function as chromophoric sensors for cations, anions, or neutral molecules. Calixfnjpyrroles, calixfmjpyridinofnjpyrroles and calixfmjpyridines as separation media Separation of closely similar molecules from highly complex mixtures requires high resolution techniques. Ion exchange, affinity chromatography, reverse phase chromatography, and gel filtration represent current types of separation techniques. The present macrocycles provide a new type of separation media due to non-covalent, primarily hydrogen bonding, interaction between the macrocycle and a guest. In ion exchange, separation is based on charges carried by the solute molecules. An ion exchanger includes an insoluble matrix or solid support to which charged groups have been covalently bound. The charged groups are associated with mobile counterions. The counter ions can be reversibly exchanged with other ions ofthe same charge without altering the matrix. It is possible to have both positively and negatively charged exchangers. Positively charged exchangers have negatively charged ion (anions) available for exchange and are so termed cation exchangers. Negatively charged exchangers have positively charges counterions and are termed cation exchangers. The matrix may be based on inorganic compounds, synthetic resins, polysaccharides, silicas, aluminas, or polymeric supports. The nature of the matrix determines its physical properties, such as its mechanical strength and flow characteristics, its behavior towards test samples, and to some extent, its capacity. Affinity chromatography is a type of adsoφtion chromatography in which the molecule to be purified is specifically and reversibly adsorbed by a complementary binding substance (ligand) immobilized on an insoluble support (matrix). Cyanogen bromide activation has been the most common method for coupling ligands to a matrix. A successful separation requires that a ligand is available and that it can be covalently attached to a chromatatographic bed material. It is also important that the immobilized ligand retain its specific binding affinity for the substance of interest and that methods are available for selectively desorbing the bound substances in an active form, after washing away unbound material. Reverse phase chromatography relies on hydrophobic surfaces, especially silylated alkyl (Rl , R2, R3, SiO), on the solid support to bind hydrophobic molecules. More polar molecules pass through the column unimpeded. The hydrophobic media may include aromatic molecules, long chain aliphatic hydrocarbons, or cyclic hydrocarbons. Separation in gel filtration depends on the different abilities of the various sample molecules to enter pores in a stationary phase. As a solute passes down a chromatographic bed, its movement depends upon the bulk flow of the mobile phase and upon the Brownian motion of the solute molecules which causes their diffusion into and out of the stationary phase. Very large molecules that never enter the pores move through the chromatographic bed fastest. Smaller molecules that can enter the gel pores move more slowly through the column since they spend a proportion of their time within the pores. Molecules are, therefore, eluted in the order of decreasing molecular size. In contrast to the known methods of chromatography, the macrocycles of the present invention provide new principles for separation of ions and molecules. Interactions between macrocycles of the present invention and ions and molecules are noncovalent, primarily hydrogen bonding. As a result, suφrising and unexpected affinities and selectivities are observed for the separation of very similar molecules. For instance, the following sets of anions or neutral molecules were successfully separated (Example 10): • Phenyl arsenate, benzene sulfonate, benzoate, and phenyl phosphate; • 5'-Adenosine monophosphate, 5'-adenosine diphosphate and 5'-adenosine triphosphate; • Uridine, phenol, aniline, benzene, and 4-iodo-nitrobenzene; • Cbz-N-protected-serine, -glutamine, -alanine, -phenylalanine, -tryptophan, -aspartate, and -glutamate; • 4-Fluorobiphenyl, 4,4'-difluorobiphenyl, 2,2',3,3 , ,5,5',6,6',-octafluorobiphenyl, and perfluorobiphenyl; A mixture of polydeoxythymidylates oligomers containing between (12) and (18) bases; • A mixture of polydeoxythymidylates oligomers containing between (19) and (24) bases; • TCTAGA, GCATGC, andCCCGGG; • Phthalate, isophthalate and teraphthalate; • Benzoate, isophthalate, and 1,3, 5-tricarboxybenzene; and • Carbofuran, carbendazim, bromacil, bentazon, carboxin, and norflurazon. A method of use of the present invention comprises separating a first molecule, a first anion or a first cation from a mixture of a first molecule, first anion or first cation, and other species, using a form of solid support-immobilized calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine by contacting the solid support with the mixture to separate a first molecule, first anion or first cation from a mixture of first molecule, first anion or first cation and other species. Examples of solid supports include, but are not limited to, silica gel, aminopropyl silica gel, carboxylalkylated silica gels, chloromethylated silica gel, chloroalkylated silica gel, other functionalized silica gels, alumina, polyacrylamide polymer beads, polystyrene polmer beads, sepharose, sephadex, agarose, clays, or zeolites coupled to calixfnjpyrroles, calixfmjpyridinofnjpyrroles or calixfmjpyridines. Examples of forms of solid supports include, but are not limited to chromatography columns, thin-layer chromatographic supports, electrophoresis gels, or capillary electrophoresis tubes. Examples of ions or neutral molecules to be separated are neutral aromatic or aliphatic species (polymeric, oligomeric and monomeric), a polyhalobiphenyl (including polychlororbiphenyl), or anionic species (polymeric, oligomeric and monomeric) such as nucleotides, oligonucleotides, pertechnetate, polyoxometalates or inorganic phosphate. The inventors' discovery that calixfnjpyrroles recognize and bind phosphate anions led the inventors to reason that such macrocycles would be ideal for use in techniques to separate and purify oligonucleotides. Data provided in Example 10 demonstrate that this is the case. The present inventors realized that for a solid support to be most useful, it must not only separate similar nucleotides and oligonucleotides from one another, it must do so rigorously and correctly based on chain length or size. Unfortunately, prior to the present invention, there were no suitable phosphate chelating agents which made this an achievable goal for use in either HPLC or electrophoretic separation systems. As described in Example 10, the inventors designed and constructed calixpyrrole-based supports and used them to achieve the separation of nucleotides and oligonucleotides, thus overcoming many of the existing drawbacks. A further utility of such calixpyrrole-substituted materials is their use as tools in the removal of phosphorylated environmental contaminents from ground water, soil, foodstuffs, and the like. They may therefore be employed to analyze and separate pesticides such as, but not limited to, carbofuran, carbendazim, bromacil, bentazon, carboxin and norflurazon. Calixpyrrole-substituted silica gels and columns may be employed in the rapid detection and analysis of organophosphorus chemical warfare agents, allowing them to be disposed of where necessary. Another technical area in which significant improvements could be made is in oligonucleotide analysis. For example, automated gene sequencing is currently carried out using either radio- or fluorescent labeled nucleic acid gel electrophoresis. This technique is limited by the requirement for either slab or tubular polyacrylamide gels. An alternative approach, currently being considered on a research basis, is to use capillary electrophoresis. Unfortunately, this is limited when it comes to achieving separations based purely on electrostatics and oligomer size. A technique, such as the present invention, which would allow for such separation would therefore represent a significant improvement in this area. A method of making a solid-supported calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine macrocycle comprising attaching the macrocycle having a functionalized group to a solid support, the solid support reactive with the functionalized group, or to a tether- functionalized solid support, the tether reactive with the functionalized group is a further embodiment of this invention. An exemplary method is described in Example 10. Two new derivatized silica gels have been produced. Gel M is aminopropyl silica gel linked to the attached calixpyrroles via a calixpyrrole meso-carbon and Gel B is aminopropyl silica gel linked to the appended calixpyrrole via a calixpyrrole β-carbon. Both gels have been demonstrated to separate mixtures of anions such as oligonucleotides (dT 12-24). nucleotides, aromatic polycarboxylates, phenyloxoanions, halides, phosphates and sulfates in an HPLC column (Example 10). Additionally, the calixpyrrole-modified silica gels can separate mixtures of neutral molecules such as polyfluorobiphenyls and other aromatic species. Thus, the calixpyrrole modified column supports offer a wide range of separatory ability. The macrocycle modified solid support in the form of a chromatography column or capillary electrophoresis tube or a contacting process involving the macrocycle modified solid support in a batch process is an embodiment of this invention. A method of forming a complex of a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole and an anion or a neutral molecule, comprising contacting the calixfnjpyrrole or the calixfmjpyridinofnjpyrrole with the anion or neutral molecule under conditions effective to allow the formation ofthe complex is another embodiment of this invention. Another embodiment of this invention is a method of transporting a molecular or ionic species through a membrane comprising incorporating a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole into the membrane; and contacting the membrane with the molecular or ionic species in the presence of a gradient of the molecular or ionic species, or a counter gradient of a further species. The transporting may result in the purification of the molecular or ionic species. Additionally binding a cation comprising contacting the cation with a calixfnjpyrrole or calixfmjpyridinofnjpyrrole having a cation-binding functionality, or with a calixfmjpyridinofmjpyrrole or calixfmjpyridine is an embodiment of this invention. Removal of pertechnetate from pertechnetate-containing nuclear waste comprising contacting the waste with a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole to form a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-pertechnetate complex; and removing the complex from the waste is an embodiment of this invention. A method of removal of an environmental pollutant from an environmental source, comprising contacting the environmental source with a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole to form a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-pollutant complex, and removing the complex from the environmental source is a further embodiment of this invention. See Example 12 for examples of removing pertechnetate from nuclear waste. Environmental pollutants such as, but not limited to nitrates, phosphates, polychlorobiphenyls, and fluoride are damaging to the environment. See Example 12 for examples of removing environmental pollutants using macrocycles. A sensor comprising a solid support bound to a macrocycle of the present invention is a further embodimnt of the present invention. A sensor can be made by either electropolymerizing the macrocycle onto the surface of the solid support or encapsulating the macrocycle into a membrane attached to the surface of the solid support is envisioned to act as an electrochemical sensor for ionic or molecular species. The calixfnjpyrrole where n is 5, 6, 7, or 8 may be associated with a metal that is bound by substituents on the periphery of the macrocycle. Heterocyclic macrocyles containing free electron pairs cheiate cations. Pyridine is known to coordinate to certain cations. Calixfmjpyridines also contain nitrogen atoms that are expected to donate electron density to cations. This donation would require that the cations stay in close proximity to the pyridine units, thereby, binding the cations to the core ofthe ring. In vivo applications Calixfnjpyrroles, calixfmjpyridinofnjpyrroles or calixfmjpyridines in DNA recognition and modification. Water-soluble calixfnjpyrroles, calixfmjpyridinofnjpyrroles or calixfmjpyridines may be particularly advantageous for use in a number of ways, such as in cellular recognition, targeting, and in the transport of biologically important molecules. Generally, water-soluble calixfnjpyrroles are preferred for biomedical applications. "Water soluble" means soluble in aqueous fluids to about 1 mM or better. Anionic phosphorylated entities are ubiquitous in biology. They play a critical role in a variety of fundamental processes ranging from gene replication to energy transduction. In addition, certain phosphate-bearing nucleotide analogues, such as, e.g., 9-(β-D- xylofuranosyl)guanine-5'-monophosphate (xylo-GMP), are known to display antiviral activity in vitro. However, xylo-GMP, like a considerable number of phosphorylated nucleotide analogues which exhibit anti-viral activity in cell-free extracts, is inactive in vivo due to its inability to cross lipophilic cell membranes. The anti-heφetic agent, acyclovir (9-[(2-hydroxyethoxy)methyl]-9H-guanine), is active in vivo. Acyclovir can enter the cell only in its uncharged nucleoside-like form. Once in the cytoplasm, it is phosphorylated, first by a viral encoded enzyme, thymidine kinase, and then by relatively non-specific cellular enzymes to produce an active ionic triphosphate nucleotide- like species. There, it functions both as an inhibitor of the viral DNA polymerase and as a chain terminator for newly synthesized heφes simplex DNA. The biological limitations of many other potential antiviral agents, including xylo-G, arise from the fact they are not phosphorylated once inside the cell and are therefore largely or completely inactive. If, however, the active monophosphorylated forms of these putative drugs could be transported into cells, it would be possible to fight viral infections with a large battery of otherwise inactive materials. If such specific into-cell transport were to be achieved, it would therefore greatly augment the treatment of such debilitating diseases as, for example, AIDS, heφes, hepatitis and measles. Given the fact that AIDS is currently a major world health problem of frightening proportions, and that something so nominally benign as measles still claims over 100,000 lives per year world- wide, treatment of these diseases would be particularly timely and worthwhile. A major aim of the inventors' studies has been therefore to provide a means of transporting active mono- and poly-phosphorylated forms of these and other agents into cells. This would allow a wide range of otherwise inactive compounds, such as antivirals, to be employed therapeutically, and would also create new possibilities for gene therapy. Calixfnjpyrrole- or calixfmjpyridinofnjpyrrole-based systems may be made effective as neutral regime carriers, say, e.g., for GMP by constructing a of polytopic receptor system in which a nucleobase recognition unit, in this case, a cytosine-like group, is appended directly to a phosphate-chelating calixfnjpyrrole or calixfmjpyridinofnjpyrrole macrocycle. Nucleobase recognition units are demonstrated herein for use in the specific binding and transport of complementary nucleobases and nucleobase-containing compounds. To synthesize multitopic receptors, calixfnjpyrrole conjugates were developed to address the following objectives: (i) the independent development of molecular recognition strategies for the complexation of two very different kinds of substrates (charged anionic and neutral nucleobase); (ii) subsequent co-combination so as to provide receptors bearing both kinds of binding subunits; and (iii) various alternative methods of receptor oligomerization so as to provide oligomeric species bearing numerous combinations of multitopic receptors. Pursuing these strategies led to the development of the calixfnjpyrrole-based ditopic receptor systems of the present invention, capable of recognizing both the anionic phosphate and the neutral portions ofthe nucleotide derivatives, such as the purine or pyrimidine moieties. The present inventors envisage expanding this theme to the preparation of oligomeric, multitopic receptors capable of recognizing multiple phosphate anions and nucleobase portions of nucleotide derivatives arranged in specific sequences. Ditopic receptor systems are ideal vehicles for the intracellular transport of oligonucleotides and their derivatives, including anti-viral agents. The multitopic receptors, likewise, are contemplated to be of use in binding to oligonucleotides and specific sections of DNA or RNA and in transporting such nucleic acid segments into cells. Pharmaceutical preparations For in vivo and ex vivo uses, calixfnjpyrroles, calixfmjpyridinofnjpyrroles and calixfmjpyridines are provided as pharmaceutical preparations. A pharmaceutical preparation of a calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine may be administered alone or in combination with pharmaceutically acceptable carriers, in either single or multiple doses. Suitable pharmaceutical carriers include inert solid diluents or fillers, sterile aqueous solutions and various organic solvents. The pharmaceutical compositions formed by combining a calixfnjpyrrole, calixfmjpyridinofnjpyrrole or calixfmjpyridine ofthe present invention and the pharmaceutically acceptable carriers are then easily administered in a variety of dosage forms. Administration may be intravenous, intraperitoneal, parenteral, intramuscular, subcutaneous, oral, or topical, with intravenous administration being preferred. Solutions of the calixfnjpyrroles, calixfmjpyridinofnjpyrroles or calixfmjpyridines in sesame or peanut oil, aqueous propylene glycol, or in sterile aqueous solution may be employed. Such aqueous solutions should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These particular aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal administration. In this connection, sterile aqueous media which can be employed will be known to those of skill in the art in light of the present disclosure. Topical creams, emulsions, solutions, and the like are contemplated for applications to surface areas of the body. Topical application may also be by iontophoresis. The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases the form must be sterile and must be fluid to the extent that easy use with a syringe exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixtures thereof, and vegetable oils. The proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars such as mannitol or dextrose or sodium chloride. Prolonged absoφtion of the injectable compositions can be brought about by the use in the compositions of agents delaying absoφtion, for example, aluminum monostearate and gelatin. Sterile injectable solutions are prepared by incoφorating the active compounds in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incoφorating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, permeation enhancers, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incoφorated into the compositions. Calixfnjpyrroles, calixfmjpyridinofnjpyrroles or calixfmjpyridines may be incoφorated into liposomes for use in the present invention. Liposomes may be prepared by any number of techniques that include freeze-thaw, sonication, cheiate dialysis, homogenization, solvent infusion, microemulsification, spontaneous formation, solvent vaporization, reverse phase, French pressure cell technique, or controlled detergent dialysis, for example. Preparation may be carried out in a solution, such as a phosphate buffer solution, containing calixfnjpyrrole-, calixfmjpyridinofnjpyrrole or calixfmjpyridine-lipophilic molecule conjugates so that the conjugate is incoφorated into the liposome membrane. Alternatively, the conjugate may be added in already formed liposomes. Liposomes employed in the present invention may be of any one of a variety of sizes, preferably the less than about 100 nm in outside diameter, more preferably less than about 50 nm. Micelles may be prepared by suspension of a calixfnjpyrrole-, calixfmjpyridinofnjpyrrole- or calixfmjpyridine-lipophilic molecule and lipid compound(s) in an organic solvent, evaporation of the solvent, resuspension in an aqueous medium, sonication and then centrifugation. Alternatively, the calixfnjpyrrole-, calixfmjpyridinofnjpyrrole- or calixfmjpyridine-lipophilic molecule may be added to preformed micelles. Techniques and Iipids for preparing liposomes and micelles are discussed in U.S. Patent 5,466,438, incoφorated herein by reference. Use of calixfnjpyrroles, calixfmjpyridinofnjpyrroles, or calixfmjpyridines in ex vivo and in in vivo treatment of body tissues. Use of macrocycles of the present invention includes the dialysis of bodily fluids. Examples of dialyzable substrates include, but are not limited to, phosphate-containing molecules or halide waste. Examples of conditions for treatment include gout, diabetes, or drug overdoses. Using kidney dialysis to illustrate an ex vivo treatment, bodily fluid would be contacted with a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine. Phosphate bound by the macroycle is removed from the blood. The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope ofthe invention. Example 1 Novel Crystal Structures of Complexed Prior-Art mesø-Substituted Calixpyrrole Macrocycles The present inventors have crystallized anion- and neutral molecule-complexed forms of known calix[4]pyrrole macrocycles and have studied the coordination properties of calixpyrroles in solution and in the solid state. As a result, the present inventors have demonstrated unexpected crystalline forms of calixpyrrole macrocycles and the existence of a conformational change between an unbound macrocycle and a macrocycle bound to an anion or a neutral substrate. In the solid state, uncomplexed calixf4jpyrrole macrocycles adopt an alternate conformation where adjacent pyrrole rings are oriented in opposite directions. Solid- state complexed calix[4]pyrrole macrocycles are shown herein to adopt a cone conformation such that the four NH protons of the pyrrole rings hydrogen bond to the bound substrate forming a supramolecular ensemble. In solution, a dynamic equilibrium exists where the bound anionic or neutral substrate is reversibly bound to all or less than all of the pyrrole NH groups. Such a conformational change would alter the properties ofthe calixpyrrole, for example, groups on the meso- or β-positions would be brought into closer proximity in the cone configuration. This conformational change may be taken advantage of in the design of a sensor, for example. meso-Octamethylcalix[4]pyrrole 1 was prepared using prior art procedures in the following manner. Pyrrole (20 g) and acetone (20 g) were introduced into a stirred 500 mL round-bottomed flask fitted with a reflux condenser. Methanesulfonic acid (1 mL) was added down the reflux condenser, causing a violent reaction. CARE! - The acid must be added slowly! Once the acid was added, the solid mass formed was broken up and a further 20 mL of acetone was added. The mixture was then heated at reflux for 30 min. After this time, the mixture was allowed to cool and the white precipitate was filtered off. The white precipitate was crystallized from acetone to give a crude crystalline material. This was recrystallized from acetone, giving crystals of the product m es o - octamethylcalixf4Jpyrrole 1, suitable for single crystal X-ray diffraction studies. meso-Tetraspirocyclohexylcalix 4 Jpyrrole 2 was prepared according to prior art procedures as follows. A solution of 16.2 g (0.242 mol) of pyrrole and 20 mL of absolute ethanol was placed in three-necked 500 mL flask. The flask, fitted with a reflux condenser, was placed in an ice bath. To the stirred solution was added dropwise 1 mL of 37% aqueous hydrochloric acid solution, followed by 23.7 g (0.242 mol) of cyclohexanone over a period of 20 min. The stirrer was stopped by the solid formed in the flask when the addition of cyclohexanone was complete. After standing at room temperature for 1 hour, the product was washed with 50 mL of cold water. The solid was removed from the flask by partial dissolution in ether. The total yield ofthe crude synthesis was 12.86 g (9.0%). Several crystallizations of the product from benzene and then from hexane gave white crystals of tetraspirocyclohexylcalix[4]pyrrole 2, m.p. 272.0 - 272.5°. 1 2 X-ray Crystallographic Data. Single crystals suitable for X-ray crystallographic analysis were obtained by slow evaporation of acetone and acetone/dichloromethane (1:1 v/v) solutions of calixpyrroles 1 and 2, respectively. The crystallographic summary for 1 is as follows. Pale yellow crystals, tetragonal, P4ι, Z = 2 in a cell of dimensions a = 10.097(1) A, c = 23.764(1) A, V =2422.7(4)A 3 , p calc = 1.18 g cm" 3 , F(000) = 928. Data were collected at 198K on a Siemens P4 diffractometer, equipped with a Nicolet LT-2 low-temperature device and using graphite monochromatized Mo Kα radiation (λ = 0.71073A). Data were collected using ω scans with a scan range of 1° in ω. 3733 unique reflections, 2552 with F 0 >4(σ(F 0 )). The final R = 0.0556, R w = 0.132, goodness of fit = 0.999 for 290 parameters. The crystallographic summary for 2 (CH2C1 2 ) is as follows. Colorless crystals, monoclinic, P2ι/c, Z = 4 in a cell of dimensions a = 10.993(2) A, b = 14.250(3) A, c, = 23.466(4) A, β = 97.254(14)°, V = 3646.5(1 1) A 3 - p calc = 1.23 g cm" 3 , F(000) = 1448. Data were collected on a Siemens P3 diffractometer. 5562 unique reflections, 2285 with F 0 >4(σ(F 0 )). The final R = 0.0762 , Rw = 0.141, goodness of fit = 0.995 for 424 parameters. As illustrated in FIG. IA and FIG. IB, molecules 1 and 2 adopt a 1,3 -alternate conformation in the solid state wherein adjacent rings are oriented in opposite directions similar to a previously-reported octaethyl analogue. Crystals of anion complexes ofthe calixpyrroles 1 and 2 were then prepared in order to study the conformational changes from the free ligand to the complexed ligand. Crystals of the tetrabutylammonium chloride complex of calixpyrrole 1 were obtained by slow evaporation of a dichloromethane solution containing an excess of the chloride salt. The crystallographic summary for l (n-Bu4NCl) CH2Cl2. is as follows: Colorless crystals, Orthorhombic, Pna2ι, Z = 4 in a cell of dimensions a = 21.247(3) A, b = 19.937(2) A, c = 10.691(1) A, V = 4528.7(9)A 3 ' p caJc = 1.16 g cm" 3 , F(000) = 1720 6027 unique reflections, 3566 with F 0 >4(σ(F 0 )). The final R = 0.0959, Rw =0.246, goodness of fit = 1.224 for 478 parameters. Crystals of the tetrabutylammonium fluoride complex of compound 2 were obtained using a similar procedure and the crystallographic summary for 2 (n-Bu 4 NF) is as follows. Colorless crystals, tetragonal, P4/nnc, Z = 4 in a cell of dimensions a = 15.732(1) A, c = 20.076(1) A, V = 4968.7(5)A 3 ' p calc = 1.14 g cπr 3 , F(000) = 1872. 3634 unique reflections, 2220 with F 0 >4(σ(F 0 )) . The final R = 0.064, Rw = 0.171, goodness of fit = 1.029 for 152 parameters. The structures so obtained (FIG. 2A and FIG. 2B) reveal that in both cases the calix[4Jpyrrole ligand adopts a cone-like conformation such that the four NH protons can hydrogen bond to the bound halide anion. While these two structures are thus grossly similar, in the case of the chloride complex (FIG. 2A) the nitrogen-to-anion distances are in the range of 3.264(7) - 3.331(7) A, while for the corresponding fluoride complex they are 2.790(2)A (FIG. 2B) (the four pyrrole groups are equivalent by symmetry). As a result, in these two complexes the chloride and fluoride anions reside 2.319(3) A and 1.499(3) A above the N 4 root mean square planes of calixpyrroles 1 and 2, respectively. Thus, fluoride anion appears to be more tightly bound in the solid state. Expanding the range of potential guests, complexes of neutral species were then prepared. Crystal complexes of calixpyrrole 1 and iV.N-dimethylformamide ("1 » 2(DMF)") were grown by dissolution of compound 1 in DMF and subsequent crystallization. The N pyrrole " -°DMF distances are 2.908(2) and 2.924(2) A, with associated N-H...O angles of 167(2)° and 166(2)°, respectively. The unsaturated portion of the amide lies 3.363(3) A above the plane of a third pyrrole ring (the planar twist angle between these two moieties is 7.1(1)°), suggesting that a π-stacking interaction helps to stabilize 1»2(DMF). The crystallographic summary for 1-2(DMF) is as follows: colorless prisms from DMF, triclinic space group PΪ, Z = 1, a = 9.473(1) A, b = 9.573(1) A, c = 10.316(1) A, α = 66.428(4)°, β = 80.987(5)°, / = 72.025(4)°, V = 815.00(14) A 3 , p calc = 1.17 g cm " 1 , (000) = 312. Final R = 0.0390, Λ w = 0.0952, GOF = 1.061 for 291 parameters. The NH protons of 1«2(DMF) were obtained from a AF map and refined with isotropic thermal parameters. Crystal complexes of calixpyrrole 1 and methanol ("l*2(CH3OH)") have also been studied by X-ray crystallography. The crystals were prepared by slow evaporation of a methanol/dichloromethane solution of the ligand. The calixpyrrole unit in l « 2(MeOH) was found to assume a 1 ,3-alternate conformation in the solid state. Single molecules ofthe alcohol lie above and below the macrocycle, each one held in place by hydrogen bonds to two pyrrolic NH groups. The four symmetry-equivalent H-bonds are 3.155(4) A long, a value which is very close to the distances (ca. 3.0-3.2 A) found in a methanol complex of a tetrapyrrolic "expanded poφhyrin." Further evidence that methanol is bound to 1, rather than merely occupying space in the lattice, is given by the inward tilt of the pyrroles of l»2(MeOH). This effect compresses the "cross ring" and Ni A " -Nι g distances by ca. 0.15 A relative to those in free 1, although it is not sufficient to allow for a linear alignment of the three hydrogen bonding atoms (Np yrro ι e -H---0 Me oH = 152.1(4)°). Crystal data for l»2(MeOH): yellow prisms from C^C^-MeOH, tetragonal space group 14, Z = 2, a = 10.383(2) A, b = 10.383(2) A, c = 13.232(5) A, =1426.6(6) A 3 , p calc = 1.15 g cm " (000) = 536. Final R = 0.0787, R w = 0.205, GOF = 1.099 for 84 parameters. The NH proton of l*2(MeOH) was calculated in an idealized postion (N-H = 0.90 A) with U lS0 set to 1.2 c7 eq for the attached N atom. Crystal complexes of calixpyrrole 1 and dimethylsulfoxide ("l » (DMSO)") have also been studied by X-ray crystallography. The crystals also undergo a phase change as they are cooled, at room temperature there is one crystallographically distinct calixpyrrole in the unit cell, at -75 "C there are two. In both phases the calixpyrrole adopts a 1 ,3 -alternate conformation wherein a DMSO molecule is hydrogen bonded to one pyrrole NH group (at low temperature N-0 = 2.962, 2.985A; H-O = 2.089, 2.024 A and N-H-0 = 176.2° and 174.5° and at room temperature N-O = 2.996A, H-O = 2.099A and N-H...O = 174.5°). Crystallographic data for l » (DMSO): There are two crystallographic modifications of l » (DMSO). The room temperature modification is monoclinic, P2ι/c, Z = 4, in a cell of dimensions: a = 10.423(2), b = 23.599(6), c = 12.480(2)A, β = 107.72(1)°, V = 2924(1 )A 3 , p ca lc = 1.15 g cm" 3 , F(000) = 1096. 6720 unique reflections, 2161 with F 0 > 4(σ(F 0 )). The final R(F) = 0.0866, Rw(F 2 ) = 0.253, goodness of fit = 0.981 for 335 parameters refined on F 2 . The low-temperature modification (- 75 °C) is triclinic, PΪ, Z = 4, in a cell of dimensions: a = 10.362(3), b = 12.373(7), c = 23.468(10)A, α = 89.77(4), β = 88.35(3), γ = 72.36(4)°, V = 2866(2)A 3 , p ca ι c = 1.17 g cm' 3 , F(000) = 1096. 10061 unique reflections, 5213 with F 0 > 4(σ(F 0 )). The final R(F) = 0.0819, Rw(F 2 ) = 0.212, goodness of fit = 1.075 for 700 parameters refined on F 2 . The complex of calix[4]pyrrole 1 and JV,N-dimethylformamide ("1«2(DMF)") indicates that individual symmetry-equivalent guests are found above and below the host, and that each amide is hydrogen bonded to adjacent pyrroles (FIG. 3B). The conformation ofthe calixpyrrole is therefore 1 ,2-alternate. To the present inventors' knowledge, 1 » 2(DMF) is the first calixpyrrole in this conformation to be unambiguously characterized. The complexes of l « 2(CH 3 OH) (FIG. 3A) and l»(DMSO) (FIG. 3C) both adopt the 1,3-alternate conformation in the solid state. However in the case of 1»2(CH30H) all the pyrrole NH groups are involved in hydrogen bonding, whereas in the case of l«(DMSO) only one pyrrolic NH group hydrogen bonds to the calixpyrrole. While cone, 1 ,3-alternate, 1 ,2-alternate and partial cone conformations for calixpyrrole macrocycles have been proposed previously, the present studies provide the first demonstration of the crystalline forms of anion-complexed molecules. Solution phase data are provided in Example 10. Example 2 Novel meso-Substituted Calix [4] pyrrole Macrocycles The present example provides meso-substituted calix [4]pyrrole macrocycles that have not previously been known. In particular, calixf4Jpyrrole macrocycles having functional groups for further derivatization have proved particularly useful as provided for in later examples. Calixf4Jpyrrole meso-monoester 3. 4-Acetylbutyric acid (3.8 g, 29 mmol) was dissolved in methanol (50 mL) in a 100 mL round bottomed flask with magnetic stirring. Concentrated hydrochloric acid (1 mL, 37%) was added and the mixture heated at reflux for 12 hr. The solvent was then removed in vacuo and the residue redissolved in dichloromethane (50 mL). The solution was then washed with saturated NaHCO3 (aq) solution (2 x 50 mL) and then brine (1 x 100 mL). The organic layer was separated and then dried over MgSO4 and reduced in vacuo to give the methyl ester, methy 1-4-acetylbutyrate, as a yellow oil (3.5g, 24.3 mmol) corresponding to a yield of 84%. 1H NMR (CDC1 3 ) 3.64 (s, 3, OCH 3 ), 2 - 49 (* > J = 7.2 Hz, 2, CH 2 ), 2.32 (t, J = 7.2 Hz, 2, CH 2 ), 2.12 (s, 3, CH 3 ), 1.86 (m, 2, CH 2 ). C NMR (CDCI3) 207.0, 172.8, 50.7, 41.7, 32.3, 29.1, 18.25. CI MS m z @ 145 (MH + ) Methyl-4-acetylbutyrate (0.80 g, 5.6 mmol) was dissolved in methanol (50 mL) in a 100 mL round bottomed flask. Cyclohexanone (0.55 g, 5.6 mmol) and pyrrole (0.75 g, 11.1 mmol) were added and the mixture cooled in an ice bath to 0°C. The flask was fitted with a reflux condenser and magnetically stirred. Methanesulfonic acid (20 drops) was added dropwise over the course of five minutes, and the mixture was stirred at 0°C for 1 hr. and then at room temperature overnight. The solvent was removed in vacuo. FAB mass spectrocopic analysis showed the presence of mono-, di-, tri- and tetrahook material in the crude reaction mixture as well as tetraspirocyclohexylcalix[4]pyrrole. The residue was purified by column chromatography on silica gel using dichloromethane as eluant. This yielded the monohook calixpyrrole methyl ester 3 as a white powder (21 lmg, 0.33 mmol) corresponding to a yield of 11.8%. iH NMR (CDCI3) 7.07 (br. m, 4, NH), 5.88 (m, 8, CHψy)), 3.63 (s, 3, OCH 3 ), 2.22 (t, J = 7.2 Hz, 2, CH 2 ), 1.93 (v. br., 14, 6CH 2(cyclohexyl) + CH 2(hook) 1.56-1.35 (v. br., 23, CH 2(hook) + 9CH 2(cyclohexyl) + CCH 3 ). 13 C NMR (CDC1 3 ) 173.8, 137.169, 136.5, 136.3, 130.7, 103.6, 103.5. 103.2, 53.4, 51.37, 39.7, 39.5, 39.4, 38.5, 37.5, 37.2, 36.9, 36.8, 34.0, 26.1, 25.9, 22.75, 22.70, 19.9. CI MS m/z @ 635 (MH + ). High Resolution Mass Spectrum: Mass = 634.4236 mDa l.l ppm 1.8 Calc. mass 634.4247 DBE 17.0 C 41 H 5 4N 4 O 2 . Calixf 4 Jpyrrole meso-monoacid 4. The calixpyrrole methyl ester (21 1 mg, 0.33 mmol) (compound 3) was dissolved in 20 mL ethanol in a 100 mL round bottomed flask with magnetic stirring. The flask was fitted with a reflux condenser and the solution heated to reflux. An aqueous solution of sodium hydroxide (20 mL, 2.0 M) was added and the mixture heated at reflux for 4 hr. The solution was then allowed to cool and the ethanol present in solution was removed in vacuo. The remaining aqueous solution was acidified to pH 1 with perchloric acid (70%). The product was then extracted from this aqueous phase with dichloromethane (50 mL). The dichloromethane layer was washed with water (100 mL) and dried over MgSO . Removal of the solvent afforded the "monohook" calixpyrrole acid 3 as an off-white powder (187 mg, 0.30 mmol) corresponding to a yield of 90.6%. l H NMR (CDC1 3 ) 7.02 (br. m., 4, NH), 5.89 (m, 8, CH (py) ), 2.26 (t, 2, CH 2 ), 1.91 (br., 14, 6CH 2(cyclohexy i ) + CH 2(hook) ), 1.44 (br. m., 24, 9CH 2(cyclohexyl) + CH 2(hook) + CCH 3 ) + 0.5H 2 O) * 3 C NMR (CDC1 3 ) 179.4, 137.3, 136.6, 136.2, 103.7, 103.6, 103.5, 103.2, 53.4, 39.7, 39.4, 39.3, 38.5, 37.4, 37.2, 36.9, 36.8, 33.8, 26.0, 25.9, 22.7, 22.6, 19.5. FAB MS m/z @ 620 (MH + ). High Resolution Mass Spectrum: Mass = 620.4073 mDa 1.7 ppm 2.8 Calc. mass 620.4090 DBE 17.0 C 4 oH 52 N 4 O 2 . X-ray quality crystals of the tetrabutylammonium salt of calix[4Jpyrrole carboxylate 4 were obtained by slow evaporation of a dichloromethane solution of 4 in the presence of excess tetrabutylammonium fluoride trihydrate. The resulting crystal structure demonstrated that this anionic calixf4]pyrrole self-assembles into dimers in the solid state forming a cyclic structure. The carboxylate moiety is hydrogen bonded to the pyrrole array of an adjacent calix[4 Jpyrrole (FIG. 4). Therefore the calix[4]pyrrole 4 adopts a cone conformation in the dimer. The separation between the root mean square planes of the nitrogen atoms in each macrocycle is 7.41(1)A. Crystallographic summary for (C 40 H 5 ιN 4 O 2 ) 1 - (Cι 6 H 36 N) 1+ - 2CH 2 C1 2 , colorless needles were grown from CH2CI2, triclinic, PΪ, Z=2 in a cell of dimensions: a = 12.682(2), b = 13.127(2), c = 17.871(2)A, α = 99.913(9), /} = 90.527(9), γ= 98.49(1)°, V= 2896.7(6)A 3 , p ca ι c = 1.18 g cm- 3 , F(000) = 1116. A total of 11364 reflections were measured, 10078 unique (R mX = 0.044) on a Siemens P4 diffractometer using graphite monochromatized Mo Kα radiation (λ = 0.71073 A). The structure was refined on F 2 to an R w = 0.220, with a conventional R = 0.084, with a goodness of fit = 1.031 for 661 refined parameters. The geometry of the interaction is: N1-H1N - Ol (related by 1-x, 1-y, 1-z), N-O 2.975(5)A, H-O 2.19(4)A, N-H-O 162(4)°; N2-H2N - Ol, N-O 2.980(5)A, H-O 2.18(5)A, N-H-O 167(4)°; N3-H3N - Ol, N-O 2.950(5)A, H-O 2.14(4)A, N-H-O 163(4)°; N4-H4N - Ol, N-O 2.916(5)A, H-O 2.09(4)A, N-H-O 176(4)°. "Monohook" acid 4 is particularly useful as a synthon for the production of calix[4]pyrrole derivatives, conjugates, modified silica gels and the like. Additionally the present inventors expect that compound 4 may be used as a zwitterion binding agent as cations may coordinate to the carboxylate functionality and anions to the calixpyrrole core. Tetravinylcalix[4Jpyrrole 5. Pyrrole (0.745 g, 11.12 mmol) and 5-hexan-2-one (1.09 g, 1 1.12 mmol) were dissolved in methanol (100 mL). Methanesulfonic acid (0.5 mL) was added and the reaction was stirred in the dark for 12 hr. The solvent was then removed in vacuo and the reaction mixture was purified by column chromatography on silica gel using dichloromethane as eluant. The first fraction from the column was collected and, upon removal ofthe solvent, afforded a colorless oil (1.05 g, 64% yield). The product 5 is a mixture of conformers of tetravinylcalix[4]pyrrole where the groups in the meso-positions are each in one of two orientations, with the bulkier alkene group either above or below the plane ofthe meso-carbon atoms (see Example 7). >H NMR (CDCI3) : 7.02 (br. s, 4H, NH), 5.91 (m, 8H, CH {pyπole) ), 5.71 (m, 4H, =CH-), 4.90 (m, 8H, =CH 2 ), 2.22 (br. m., 16H, CH 2 CH 2 ). 13 C NMR (CD 2 C1 2 ) : (139.4, 139.3, 139.2), (137.83, 137.80, 137.70, 137.63, 137.58), (1 14.5, 1 14.4, 114.35), (104.4, 104.3, 104.26, 104.2, 104.1, 104.0), (40.02, 39.98), (39.2, 39.0), 29.2, (26.45, 26.43, 26.23, 26.15, 26.04, 25.97). 5 Calix[4Jpyrrole 5 may prove to be particularly useful as a monomer for use in the synthesis of calixpyrrole polymer materials. A known method uses pyrrole, a C4-C6 saturated alicyclic ketone and an acid containing vinyl groups or triple bonds to form a polymerized resin. In this case, the resulting products are undefined since it appears to be unknown where the modifying group is attached to the product. In contrast, the present method allows synthesis of monomer 5 which can then be subjected to polymerization. This method of forming a defined calixfnjpyrrole polymer is applicable for n = 4 - 8. Meso-octabenzylcalixf4Jpyrrole 6. Pyrrole (0.745 g, 11.12 mmol) and 1,3- diphenylacetone (2.34 g, 11.12 mmol) were dissolved in methanol (250 mL). Methanesulfonic acid (0.5 mL) was added and the reaction was stirred in the dark for twelve hours. The solvent was then removed in vacuo and the reaction mixture was purified by column chromatography. The first fraction from the column was collected and, upon removal of the solvent, afforded a yellow oil. Acetone (10 mL) was added and the flask was swirled in order to dissolve the oil. After 20 min, white crystals ofthe meso-octabenzylcalix[4jpyrrole 6 (278 mg, 9.6% yield) had formed; they were collected and dried. iH NMR (CDC1 3 ) : 7.19 (m, 24H, ArH), 6.83 (m, 16H, ArH), 6.22 (br. s, 4H, NH), 5.62 (d, 8H, CH (pyrτole) ), 2.90 (m, 16H, ArCH 2 . C NMR (CDCI3) : 138.3, 134.1, 130.9, 127.7, 126.4, 106.5, 44.7. High Resolution negative FAB calc. for C 76 H 67 N 4 (1035.53674) found 1035.4720 (1.8 ppm). Crystals of octabenzylcalixf4jpyrrole 6 suitable for X-ray study were grown from acetone. The conformation ofthe macrocycle in 6»2(acetone)»acetone |atUce is 1,3-alternate (as in l » 2(MeOH)), but the two acetone guests are both located on the same face ofthe ligand, held in place by single H-bonds (FIG 5). One of the acetone moieties lies much closer to the macrocycle than the other (Np yrτo ι e - acetone = 2.972(6) A vs. 3.359(6) A), reflecting an almost colinear (177(5)°) arrangement of N 0je -H...O acetone atoms in the former case. By contrast, the N-H- O angle formed with the more distant ketone measures 159(4)°. Octabenzylcalixf4Jpyrrole may prove useful as a phase transfer catalyst for anions, or as a starting material for the synthesis of calix[4]pyrrole aryl-transition metal complexes which could be used as catalysts for various reactions or as a sensor for anions. This material is also a potential precursor to pyridine-containing systems (Example 6). meso- Tetraspirotetrahydrothiopyrancalixf4]pyrrole 7. Tetrahydrothiopy ran-4-one (0.50g, 4.3 mmol) and pyrrole (0.288g, 4.3 mmol) were dissolved in ethanol (30 mL) and 10 drops of methanesulfonic acid were added. The mixture was stirred for 12 hours in the dark, after which time a white precipitate had formed. The solvent was removed in vacuo and the residue purifed by column chromatography (SiO 2 , CH 2 C1 2 eluant) affording the tetra sulfide macrocycle as a white powder (540 mg, 76 % yield). >H NMR (250 MHz, CD 2 C1 2 ) δ: 7.10 (s, 4H, NH), 5.97 (m, 8H, CHp yrro ie), 2.61 (m, 12H, CH pyra n) 2.28 (m, 12H, CH pyran ). 13 C NMR (62.90 MHz, CD 2 C1 2 ) δ: 136.0, 105.0, 39.5, 37.9, 24.9. High resolution FABMS calc for C 36 H 4 4N 4 S 4 660.2449; found 660.2437 (Δ 1.7 ppm). Crystals of macrocycle 7 suitable for analysis by X-ray diffraction techniques were grown by slow evaporation of a dichloromethane solution of the macrocycle (FIG. 6). Crystallographic data for 7: Colorless crystals, triclinic, PΪ, Z = 2, in a cell of dimensions: a = 1 1.059(2), b = 15.023(3), c = 15.874(3 )A, α = 112.21(1), β = 102.64(1), γ = 103.07(2)°, V = 2238.9(7)A 3 , pcaic = 1.42 g CUT 3 , F(OOO) = 998. 3987 unique reflections, 2991 with F 0 > 4(σ(F 0 )). The final R(F) = 0.0545, Rw(F 2 ) = 0.126, goodness of fit = 1.133 for 336 parameters refined on F 2 . Calix[4Jpyrrole 7 is a useful synthon for the synthesis of transition metal complexes on the periphery ofthe calixpyrrole which complexes are contemplated to be useful as sensors. Oxidation of macrocycle sulfide groups to sulfoxide or sulfone introduces further polarity into the macrocycle and renders it more water soluble, thus allowing use in biomedical applications (see Example 11). Additionally, the present inventors envision that analogous macrocycles containing NH, NH2 + , NHCH3 + , N(CH3)2 + , NOH, NNH2 and NN(CH3)2 functionalities instead of sulfur will be water soluble. meso-Octanonylcalix[4 Jpyrrole 8. 10-Nonadecanone (3.14g, 11.12 mmol) and pyrrole (0.745g, 11.12 mmol) were dissolved in a mixture of ethanol (100 mL) and dichloromethane (50 mL). Methanesulfonic acid (20 drops) was added and the mixture was stirred for 12 hours. The solvent was then removed in vacuo and the product was purified by column chromatography on silica gel (hexane eluant) affording the product as a white powder (650 mg, 17 % yield). >H NMR (250 MHz, CDC1 3 ) δ: 6.99 (s, 4H, NH), 5.86 (s, 8H, CH), [1.77, 1.54, 1.21, 0.86 (br. multiplets, 152H, CH 2 ,CH 3 ). 13 C NMR (62.90 MHz, CDCI3) δ: 136.2, 104.6, 42.4, 37.7, 31.9, 30.3, 29.78, 29.75, 29.4, 24.0, 22.7, 14.1. High resolution FABMS calc for C 2H165N 4 1326.3034; found 1326.3015 (Δ 1.4 ppm). Due to hydrophobic alkyl groups, calixpyrrole 8 is designed to have particular affinity for lipid bilayer membranes and is expected to serve as an insertion agent, i.e. insertion of macrocycle 8 is expected to allow passage of charged species (such as phosphorylated anti-viral agents) through cell membranes, thereby increasing the effectiveness ofthe agent (Example 11). meso-Tetramethyl-mesotetraferrocenylcalix[4Jpyrrole 9. Pyrrole (0.373 g, 5.56 mmol) and acetylferrocene (1.27 g, 5.56 mmol) were dissolved in methanol (50 mL) and stirred. Methanesulfonic acid (20 drops) was added and the reaction stirred for twelve hours. Triethylamine (30 drops) was then added to the reaction mixture followed by aspartic acid (2g) and the solvent removed in vacuo. The residue was purified by column chromatography (silica gel, CH 2 C1 2 eluant) yielding the product as a yellow foam (390 mg, 25% yield). l U NMR (CD 2 CI 2 ) 1.801-1.997 ppm (12H, m, CH 3 ), 3.931-4.198 ppm (36H, m, C 1 0H9 (ferrocene)), 5.625-5.927 (8H, m), 7.929 (s, 4H, NH). 13 C NMR (CD 2 C1 2 ) 28.958, 31.016, 39.841, 39.857, 67.343, 67.525, 67.818, 69.031, 69.141, 69.356, 98.224, 104.588, 104, 932, 105.814, 136.866, 137.348, 137.482, 137.572 9 Calixf4Jpyrrole 9 can be used as a reporter molecule or sensor for detecting anions or neutral molecules. The complexation of an anion in the calixpyrrole core will perturb the electronic states of the ferrocene moieties, thereby causing a shift in the ferrocene redox potential which can be detected by electrochemical techniques, including, but not limited to, cyclic volatmmetry and Osteryoung-type square wave voltammetry. While the present example demonstrates ester (3), acid (4), alkenyl (5), aryl (6), sulfide (7), and alkyl (8) substituents for calix[4Jpyrroles, one of skill in the art would be able to synthesize further meso-substituted calix[4]pyrrole macrocycles in light of the teachings herein and using standard organic chemical synthesis reactions as described herein. Example 3 Novel β-Substituted CaIix[4J pyrrole Macrocycles Calix [4Jpyrrole macrocycles with functional groups in the β-position (i.e., on the β- carbon of a pyrrole ring), and the syntheses thereof, are provided in the present example. Such β-substituted calixpyrroles may have substitution at one or more of the β-positions. To prepare such compounds of the present invention, either me-ro-octamethylcalix[4Jpyrrole 1 is deprotonated and the substituent to be added at the β-position is then reacted with the activated calixpyrrole, or the calixpyrrole is synthesized from a β-substituted pyrrole. Initially, sodium hydride was tried as a base to deprotonate the calixpyrrole. Sodium hydride was added to a solution ofthe macrocycle in DMF, after which bromoethylacetate was added in order to introduce an ester functionality to the calixpyrrole. However, this produced no reaction. A stronger base (rc-BuLi) was then tried; 4 equivalents of the base were added dropwise to a THF solution ofthe macrocycle at -78°C under an argon atmosphere. After 2 hours stirring at -78°C, 4 equivalents of bromoethylacetate were added and the solution was allowed to warm up to room temperature over 90 minutes. The THF was then removed in vacuo and the residue was purified using column chromatography on silica gel by eluting with CH 2 C1 . Several fractions were isolated from the column. The first proved to be unreacted calix[4Jpyrrole. The second was the β-monosubstituted ester 10 and the third, the β-diester 11. 1 0: R Ϊ = CH 2 COOEt, R 2 = H 1 1: R 1 = R 2 = CH 2 COOEt Calixf4Jpyrrole β-mono- and disubstituted ester 10 and 11. meso- Octamethylcalixf4Jpyrrole ( 1.0 g, 2.34 mmol; preparation described in Example 1) was dissolved with stirring in THF (100 mL, dry) under an argon atmosphere. The solution was cooled to -78°C using a CO 2 /acetone bath. n-Butyllithium (5.83 mL, 9.34 mmol, 1.6 M in hexane) was added to the solution dropwise. After 2 hours, ethylbromoacetate (1.95 g, 1 1.68 mmol) was added dropwise to the solution. The reaction was then removed from the CO 2 bath and allowed to warm to room temperature. After 90 minutes, the solvent was removed in vacuo and EtOH (100 mL) was added to the flask. Water (100 mL) was slowly added with swirling, causing a white precipitate to form. The precipitate was collected by filtration, dried under high vacuum and then purified by column chromatography on silica gel eluting with CH 2 C1 2 . The monoester 10 (310 mg, 26%) and diester 11 (40 mg, 2.8%) were the second and third main products to be eluted from the column. Mono-ester 10: •H NMR (500 MHz, CD 2 C1 2 ) δ: 8.55 (s, IH, NH), 7.15 (s, IH, NH), 7.04 (s, IH, NH), 7.00 (s, IH, NH), 5.94 - 5.88 (overlapping multiplets, 5H, CH py ), 5.83 (m, IH, CH py ), 5.72 (m, IH, CH py ), 4.24 (q, J = 7.15 Hz, 2H, CH 2 CH 3 ), 3.65 (s, 2H, CCH 2 ), 1.62-1.47 (overlapping singlets, 24H, CCH 3 ), 1.33 (t, 3H, J = 7.15 Hz). 1 C (DEPT) NMR (500 MHz, CD 2 C1 2 ) δ: 174.93 (C=O), 140.42 (C py ), 139.69 (C py ), 139.31 (C py ), 138.92 (C py ), 138.50 (C py ), 137.98 (C py ), 137.20 (C py ), 134.06 (C py ), 109.95 (CH py ), 107.07 (CCH 2 COOEt), 103.41 (CH py ), 103.10 (CH py ), 102.90 (CH py ), 102.66 (CH py ), 101.37 (CH py ), 61.40 (CH 2 CH 3 ), 37.16 (CCH 3 ), 35.50 (CCH 3 ), 35.43 (CCH 2 ), 35.17 (CCH 3 ), 33.95 (CCH 3 ), 29.20 (CCH 3 ), 29.05 (CCH 3 ), 28.90 (CCH 3 ), 28.68 (CCH 3 ), 14.47 (CH 2 CH 3 ). High resolution FABMS calc for C 32 H 42 N 4 O 2 514.3308; found 514.3302 (Δ 1.2 ppm) Bis-ester 11: Η NMR (500 MHz, CD 2 C1 2 ) δ: 8.61 (s, IH, NH), 8.52 (s, IH, NH), 7.20 (s, IH, NH), 7.00 (s, IH, NH), 5,85 - 5.54 (several multiplets - overlapping, 4H, CH p y), 5.63 (d, IH, CH py ), 5.55 (d, IH, CH py ), 4.19 (m, 4H, CH 2 CH 3 ), 3.60 and 3.59 (two singlets, total 4H, CCH 2 ), 1.58 (s, 6H, CCH 3 ), 1.52 (s, 6H, CCH 3 ), 1.49 (s, 6H, CCH 3 ), 1.44 (s, 6H, CCH 3 ), 1.29 (t, 2H, CH 2 CH 3 ), 1.27 (t, 2H, CH 2 CH 3 ). 13 C (DEPT) NMR (500 MHz, CD 2 Cl 2 ) δ: 175.51 (C=O), 175.21 (C=O), 141.21 (C py ), 140.36 (C py ), 138.98 (C py ), 137.94, (C p y) 137.70, (C py ) 137.68 (C py ), 134.33 (C py ), 133.89 (C py ), 109.64 (C py CH 2 ), 109.09 (C py CH 2 ), 106.73 (CH py ), 105.08 (CH py ), 103.23 (CH py ), 102.90 (CH py ), 101.93 (CH p y), 100.72 (CHpy), 61.42 (CH 2 CH 3 ), 37.21 (CCH 3 ), 36.89 (CCH 3 ), 35.52 (CCH 3 ), 35.19 (CCH 3 ), 33.97 (CCH 2 ), 33.93 (CCH 2 ), 29.05 (CCH 3 ), 28.90 (CCH 3 ), 14.41 (CH 2 CH 3 ). High resolution FABMS calc for C 36 H 48 N 4 O 4 600.3676; found 600.3663 (Δ 2.1 ppm). This reaction may also be accomplished using other bases including but not limited to aryllithium or alkyllithium or in the presence of a coordinating ligand such as but not limited to tetramethy lethy lenediamine with an aryllithium or alkyllithium base. Alternately other electrophiles may be attached to the activated calixpyrrole macrocycle (e.g. CO ι ethylchloroformate, acrylonitrile). β-Substituted calix f 4 Jpyrrole esters are useful as a reagent for the synthesis of other calixpyrrole derivatives as described herein. Calixf4 Jpyrrole β-acid 12. Compound 10 was de-esterified to produce calixf4]pyrrole β-acid 12 by refluxing in a solution of NaOH in EtOH/H 2 O. The acid was clarified by adding perchloric acid to the cooled reaction solution and then collected by filtration. This yielded a white powder. 1 0 1 2 Compound 10 (200 mg, 0.38 mmol) was stirred in 20 mL ethanol. The slurry was heated to reflux and NaOH a „ (20 mL, 2.0 M) was added. The reaction was heated at reflux until all the ester had been consumed (approximately 4 hours). The ethanol was then removed in vacuo and a further 50 mL of cold water was added to the solution. The solution was then acidified with concentrated perchloric acid to pH 1. The monoacid 12 then precipitated out as a white powder, was collected by filtration and dried under high vacuum to yield 146 mg (79%). l H NMR (500 MHz, CD 2 C1 2 ) δ: 7.84 (s, IH, NH), 7.24 (s, IH, NH), 7.01 (coincident singlets, 2H, NH), 5.90 - 5.76 (overlapping multiplets, 5H, CH py ), 3.71 (s, 2H, OCH 2 ), 1.51 (s, 6H, CCH 3 ), 1.50 (s, 6H, CCH 3 ), 1.48 (s, 6H, CCH 3 ), 1.47 (s, 6H, CCH 3 ). 13 C NMR (500 MHz, CD 2 Cl 2 ) δ: 179.13 (C=O), 139.96 (C py ), 139.20 (C py ), 139.09 (C py ), 138.60 (C py ), 138.52 (C p y), 137.45 (Cpy), 133.96 (C py ), 109.27 (C py ) 107.1 1 (CH), 103.21 (CH), 103.14 (CH), 103.05 (CH), 102.91 (CH), 102.75 (CH), 102.14 (CH), 37.20 (CCH 3 ), 35.49 (CCH 3 ), 35.40 (OCH 2 ), 35.23 (CCH 3 ), 33.68 (CCH 3 ), 30.38 (CCH 3 ), 29.16 (CCH 3 ), 29.09 (CCH 3 ), 29.00 (CCH 3 ), 28.85 (CCH 3 ). High resolution FABMS calc for C 30 H 38 N 4 O 2 486.2989; found 486.2995 (Δ 1.1 ppm). β-substituted "monohook" calix[4Jpyrrole 12 is particularly useful for further substitution as described in Examples 8, 9, 10, and 1 1. β-Octamethoxy-meso-tetraspirocyclohexylcalix[4Jpyrrole 13. 3 ,4-Dimethoxypyrrole (0.568g, 4.5 mmol) and cyclohexanone (437 mg, 4.5 mmol) were dissolved in glacial acetic acid (10 mL), and the solution was stirred for 3 hours in the dark under argon, forming a black solution. The acid was then removed in vacuo and the residue purified by column chromatography (SiO 2 , CH C1 2 eluant). The product was collected as the second fraction from the column having an Rf = 0.33 (81 mg, 0.097 mmol) in a yield of 8.6%. Following chromatographic purification, the solvent was immediately reduced in vacuo and the product 13 stored under argon in the freezer. »H NMR (300 MHz, CD 2 C1 ) δ: 6.11 (s, 4H, NH), 3.77 (s, 24H, OCH 3 ), 2.08 (s, br., 16H, CH2), 1.45 (s, br., 24H). l C NMR (75.42 MHz, CD 2 C1 2 ) δ: 136.3, 75.73, 61.1, 42.5, 35.1, 26.3, 23.9. High resolution FABMS calc for C 4 gH 6 8N 4 O 8 828.5037 found 828.5025 β-Substituted calix[4]pyrrole 13 shows decreased anion binding affinity relative to compound 2 due to electron donating properties of the methoxy substituents. These types of compounds are useful as HPLC column media since low affinity constants and fast complexation-decomplexation rates generally lead to efficient separation of substrates (see Example 10). β-Octabromo-meso-octamethylcalix[4Jpyrrole 14. An oven-dried 100 mL round- bottomed flask containing a stir bar was charged with meso-octamethylcalixf4]pyrrole (0.69 g, 1.6 mmol) and 50 mL of dry tetrahydrofuran. With stirring, N-bromosuccinimide (2.34 g, 13.1 mmol) was added all at once, causing the reaction mixture to turn yellow. A reflux condenser and argon balloon were affixed to the flask, which was heated to gentle reflux in the dark. After 48 h, the clear red-brown solution was allowed to cool to room temperature, and the THF was removed in vacuo. The tan solid which remained was triturated with 20 mL of dichloromethane, and the CH 2 CI 2 solution was flash chromatographed on silica gel (1:1 CH 2 Cl 2 -hexanes as eluent). Colorless-to-light-yellow fractions with R f = 0.73 were combined and evaporated to afford a white solid. Drying under high vacuum gave 1.53 g (90%) of the desired product 14. 'H NMR (CDCI 3 ): δ 1.56 (s, 24H), 7.34 (br s, 4H). "C NMR (CDCI 3 ): 525.9, 38.2, 98.6, 130.4. LRMS (FAB + ): m/z 1059, 979, 901, 821, 741. Elemental analysis calc. for 142(DMSO) C, 31.61; H, 3.32; N, 4.61. Found C, 32.18; H, 3.31; N, 4.59. Single crystals of compound 14 were grown by slow evaporation of a dilute dichloromethane solution of the macrocycle (FIG. 7). The structure reveals that compound 14 exists in a chair-like flattened 1,2-alternate conformation in the solid state (i.e., the dihedral angles between pyrrole rings and plane through the calixpyrrole meso-carbon atoms are 66.8°, 5.8 ° , -66.8° and -5.8°). Crystallographic summary for C 2 gH gN Brg: Large, colourless prisms were grown by slow evaporation from CH 2 C1 2 . Crystals were monoclinic, P2[/n Z = 2, in a cell of dimensions: a = 10.819(4), b = 12.042(4), c = 12.746(4) A, β = 99.01(2)°, V = 1640.1(10)A 3 , Pcalc = 2.15 gem" 3 , μ = 98.07 cπr 1 , F(000) = 1008. A total of 7416 reflections were measured, 3763 unique (Rin t (F 2 ) = 0.091) on a Siemens P3 diffractometer using graphite monochromatised Mo Kα radiation (λ = 0.71073A) at -75 °C. The structure was refined on F 2 using SHELXTL/PC (ver 5.03, Siemens Analytical X-ray Instruments, Madison, Wl, USA) to an R w = 0.131, with a conventional R = 0.0575 (2231 reflections with F 0 > 4[σ(F 0 )j), and a goodness of fit = 1.105 for 182 refined parameters. The molecule lies on a crystallographic inversion centre at 0,0,0. β-Substituted calix[4 Jpyrrole 14 shows increased anion binding affinity relative to the 'parent' calixf4 Jpyrrole 1 due to the electron withdrawing properties of the bromine substituents (see Example 10). Receptors with an increased anion binding ability such as 14 are useful as anion sequestering agents (e.g., in removal of phosphate and nitrate pollutants from aqueous environments) (see Example 12) or as synthons in reactions to produce further modified calix[4Jpyrroles. The present inventors envision this system will be useful as a precursor to a calixfmjpyridinofnjpyrrole or a calixfmjpyridine. While the present example describes ester (10,11), acid (12), alkoxy (13) and halo (14) β-substituents, one of skill in the art would be able to synthesize further modified β-substituted molecules in the light of the present disclosure and using standard organic chemical synthesis reactions as described herein. β-Substituted calix[4jpyrrole macrocycles have similar utility to that of the meso- substituted molecules described herein. Example 4 Use of Calixfnjarene in the Synthesis of Calixfnjpyrrole, and Pseudo Dimers Thereof The present example provides the synthesis of a calixfnjarene-calixfnjpyrrole pseudo dimer. Reaction of -fer/-butylcalix[4Jarene tetramethyl ketone (0.5 g, 0.57 mmol) with pyrrole (0.153 g, 2.29 mmol) in a dichloromethane/ethanol mixture, in the presence of methanesulfonic acid, followed by column chromatography on silica gel (CH 2 C1 2 , eluant) afforded the caiixpyrrole-calixarene pseudo dimer 15 in 32% yield. Compound 15 gave spectroscopic and analytical data in accord with the assigned structure. As a consequence of this template synthesis, the stereochemistry at the calixpyrrole mesσ-carbons is automatically defined. Thus this method of synthesis produces only one configurational isomer even though all four ketone groups involved in the synthesis of an individual calixpyrrole are asymmetrical (see Example 7). Analytical and spectroscopic data for compound 15: Analysis for C 72 Hg N 4 O 4 : Calculated C 80.86%; H 7.92%; N 5.24%. Found C 80.57%; H 7.95%; N 5.1 1%. High resolution positive ion FABMS: Calculated (C 72 H 8 N O ) 1068.649; Found 1068.648 (Δ 1.4 ppm). 'H NMR (CD 2 C1 2 ) δ: 11.22 (s, 4H, NH), 7.22 (s, 8H, ArH), 5.83 (d, J = 2.5 Hz, 8H, CH), 4.35 (d, J = 12.5 Hz, 4H, ArCH 2 Ar), 3.80 (s, 8H, OCH 2 ), 3.37 (d, J = 12.5 Hz, ArCH 2 Ar), 1.58 (PyCCH 3 ), 1.18.(s, 36H, fer/-Bu). 13 C NMR (CDC1 3 ) δ: 151.3, 146.8, 137.2, 134.4, 126.1, 101.7, 90.2, 37.9, 34.1, 31.4, 29.6, 21.1. Crystals were obtained by slow evaporation of a dichloromethane solution of compound 15. The crystals were then subject to X-ray diffraction structural analysis. The resulting structure shows the calixarene in a cone conformation. The pyrrole NH groups are within hydrogen bonding distance ofthe lower rim calixarene oxygen atoms (FIG. 8). Crystallographic summary for 15.xCH 2 Cl 2 . Large regions of disordered solvent of unknown stoichiometry in this structure presented difficulties with this analysis, however the structure of the calixarene-calixpyrrole pseudo dimer is a reliable description of this unique and interesting molecule. Faintly yellow crystals, monoclinic, C2/m, Z = 4 in a cell of dimensions a = 25.836(5)A, b = 20.139(3)A, c = 15.46(2)A, j8 = 106.0(1)°, F= 7719(2)A 3 . p ca | c : = 4; 1.21 gem -3 ; x = 6, 1.36 gcπr 3 . 91 12 unique reflections of which 4121 were observed (I > 2σ(I)). Data collected at -90°C on a Siemens P3 diffractometer. The final R = 0.148. The ' H NMR spectrum of 15 in dichloromethane-^ shows that the pyrrole NH protons, which are normally observed at around 7 ppm in other calix[4Jpyrroles, resonate at 1 1.22 ppm (FIG. 9). This can be attributed to hydrogen bonding between the pyrrole NH groups and the calixarene oxygen atoms. CD3OD was added to the NMR solution in an attempt to disrupt this hydrogen bonding interaction, however this had a negligible effect on the [ H NMR spectrum. Similarly, addition of 10.0 equiv. tetrabutylammonium fluoride (which has been shown to bind strongly to other calix[4Jpyrroles) caused no changes in the NMR spectrum. Based on these findings, the present inventors conclude that the dimer is adopting a cylindrical conformation in solution due to these clearly favorable NH - O hydrogen bonds. acid These types of calixarene-calixpyrrole molecular cylinders may be useful as receptors of neutral guests, as they possess a hydrophobic cavity. Additional functional groups could be appended to the free phenolic OH groups on the calixarene. These may be used to attach the molecule to a polymer or other solid support. Such solid supported compounds may be used in column chromatography, for example. Alternatively, an optical or electrochemical sensing group such as anthracene or ferrocene could be attached and may be able to sense the inclusion of neutral species in the cylinder and therefore act as a sensor. These materials may also be able to extract toxic organic substances (such as insecticides and PCBs) from aqueous solutions and, being potential receptors for zwitterions, aid in the purification of amino acids, peptides, proteins, and the like. In addition to a dimer cylinder, trimers and larger tubular polymers may be prepared. The synthesis of a calixarene/calixpyrrole/calixarene pseudo trimer 16 is illustrated below: Calixf4Jarene tetra ketones in the 1,3-alternate conformation may be used to make calixarene- calixpyrrole tubular polymers which may have interesting properties, either alone or with a guest species bound within the polymer tube. Example 5 Synthesis of Calixfnjpyrroles Calixfnjpyrroles where n is greater than 4 have not previously been described. The present example provides methods for synthesis of higher order cyclic oligomers where n is 5 - 8. Prior art methodology for preparing calix[4]pyrroles involved the condensation of a ketone with a pyrrole using a Brønsted acid such as methanesulfonic acid or hydrochloric acid. This method produces exclusively calix[4Jpyrrole. The present inventors have developed a methodology that employs Lewis acid catalysis of the condensation of pyrroles and ketones to yield novel calix[5, 6, 7, or δjpyrroles having high yield and purity. Synthesis of Calixfnjpyrroles where n = 5, 6, 7, or 8. A pyrrole and a ketone may undergo Lewis acid-catalyzed condensation to form a generalized calixfnjpyrrole having structure I. The reaction conditions have been optimized for the preparation of higher calixfnjpyrroles (n>4) by varying pyrrole and the ketone ratios and employing BF3.Et 2 O as a catalyst. Optimal conditions for the formation of higher order calixfnjpyrroles (n > 4) are a 1 : 1 to a 1.25 : 1 molar ratio (ketone :pyrrole) with a 10% Lewis acid catalyst. The concentration of starting compounds ranged from 50 mM to 1 mM depending on the solvent used e.g. dichloromethane (dry), chloroform and acetonitrile, although it may be possible to use other solvents or solvent mixtures to obtain optimum yields of a given calixfnjpyrrole. Lewis acids such as BF3-OEt 2 , BF3-methanoI, VOCI3, TiCl , and Mn(OAc)3 have been used as catalysts and it has been found that TiCl in dry dichloromethane produces higher yields of calixfnjpyrroles than the other catalysts. Lewis acids may also be used in doped zeolites, monymorillonites, clays, silica and alumina which can provide a convenient site for calixfnjpyrrole formation. The conditions for the preparation of calixfnjpyrroles ranging from n = 4 to 10 are: acetone (0.5808 g, 10 mmol) and pyrrole (0.67 g, 10 mmol) were dissolved in dry dichloromethane ( 150 mL) under an argon atmosphere with stirring under argon for 15 minutes. The catalyst, BF 3 .Et 2 O (0.5 mL of IM solution in dichloromethane, 0.5 mmol), was slowly added via syringe. The reaction mixture was stirred under argon for between 24 to 48 hours. HCl (3 mL of 3 %) was then added and the mixture stirred for 10 minutes. The reaction mixture was transferred to a separatory funnel and the organic phase was washed with water and dried over sodium sulfate. The solvent was evaporated and the crude reaction mixture was filtered over a short neutral alumina column using dichloromethane as the mobile phase. The calixfnjpyrrole products were purified by flash chromatography or by preparative HPLC using dichloromethane-hexane (3:1) a mobile phase. Yield 78 % The ratio for n is as follows: 1, n = 4, 60 %; 17, n = 5, 12 %; 18, n = 6, 9%; 19, n = 7, 5%; 20, n = 8, 3%. Separated calixfnjpyrroles were characterized by HRMS: Cyclic pentamer: 17, n = 5, For C35H45N5 calc. 535.367497, found. 535.367194. For [MH + ] C 35 H 46 N 5 calc. 536.375322, found 536.374278. Cyclic hexamer: 18 n = 6 For C 4 H 54 N 6 calc. 642.440996, found 642.440390. Cyclic heptamer: 19 n = 7 For C 4 9H63N7 calc 749.514496, found 749.514133. Cyclic octamer: 20 n = 8 For C5 6 H 73 Ng calc. 857.5958, found 857.5923. Chloroform and acetonitrile may also be used as solvents. BF3.MeOH was used as a catalyst with 73 % overall yield. When 1 -alkyl-, or 1 -arylpyrrole were used, the reaction again produced cyclic systems; N-substituted calixfnjpyrroles where n is from 4 to 8, with the main product n = 4, but with higher ratio for n>4 than in case of pyrrole (the ratio estimate is 35: 12: 9: 7 : 5) for n = 4 - 8, for 1 -methy lpyrrole and ratio 29:12:9:8:6 for 1-phenylpyrrole. The procedure for the use of high valent Lewis acid catalysts (e.g. TiCl ) in calixfnjpyrrole synthesis is as follows: acetone (0.581 g , 10 mmol) and pyrrole (0.67 g, 10 mmol) were dissolved in 100 mL of dry dichloromethane under argon. Titanium tetrachloride (1 mL of IM solution in dichloromethane, 1 mmol) was then added to the solution under argon while stirring at room temperature. Immediately after addition, a precipitate was formed, which redissolved after 5 minutes stirring. The reaction mixture was stirred at room temperature for 20 hours, then washed with 3 % HCl, water and dried over sodium sulfate. The product was purified by column chromatography on neutral alumina. Final separation was performed by flash chromatography on silica gel using dichloromethane-hexane 4: 1. The overall yield was 67% with a calixfnjpyrrole distribution as follows: 88 (1, n = 4), 8 (17, n=5), 6 (18, n=6), 3 (19, n = 7), 2 (20, n=8). The procedure has been repeated using analogous conditions to those used with titanium tetrachloride but using other Lewis acids such as VOCI3, Mn(OAc)3, SnCLj, AICI3, or UO2(OAc)2 and also using a number of different solvents. A variety of cation exchange resins (Dowex) were employed in their H + rich forms, however, the product was exclusively the cyclic tetramer. Synthesis of Calixfnjpyrroles using clays. Montmorrilonite K10 (Aldrich) was added to a 250 mL flask and dried at 120°C / 0.2 Torr for 2 hours. After cooling to room temperature under argon,dry dichloromethane (100 mL) was added, followed by acetone (0.0581 g, 1 mmol) and pyrrole (0.067g, 1 mmol) successively. The reaction mixture (suspension) was vigorously stirred under argon for 8 hours. The solid material was filtered through Celite and washed with dichloromethane (25 mL). The combined dichloromethane filtrates were evaporated and the product was purified on a short neutral alumina column (dichloromethane eluant). The products separated by preparative HPLC on a silica gel column using dichloromethane-hexane as the mobile phase. Overall yield was 68% with the main product being calix [4Jpyrrole 1 (59%). Additionally, calixf5]pyrrole 17 (6% yield and 3% of higher oligomers (n > 6)) were isolated. Synthesis of calixfnjpyrroles via cyclization of 2-f(dialkylhydroxy)methylJpyrrole Calixfnjpyrroles may also be synthesized from 2-f(dialkylhydroxy)methyljpyrroles. These molecules are cyclized using either a Brønsted acid or a Lewis acid. One approach for the preparation of 2-[(methylalkyl)hydroxymethyljpyrrole derivative is based on the reaction of commercially available 2-acetylpyrrole with alkylmagnesiumbromides (Grignard reagents) affording the desired product 21 (R = alkyl) as a starting building block for Brønsted acid, or Lewis acid catalyzed cyclization. 2-Acetylpyrrole (0.109 g, 1 mmol) was dissolved in 50 mL of dry THF under argon. The solution was then cooled to -78°C. A solution of MeMgBr (1 mL of a 1 M solution in diethylether) was added via syringe while stirring. The reaction mixture was allowed to warm up to -15°C for 10 minutes. It was then cooled down and another 1.5 molar equivalents MeMgBr (1.5 mL of IM solution; 1.5 mmol) was slowly added at -78°C. The reaction mixture was stirred at that temperature for 15 minutes and then slowly allowed to warm up to 0°C. After stirring for two hours at 0°C, the reaction was quenched with MeOH and evaporated to dryness. The residue was redissolved in dichloromethane (50 mL) and washed with a 5% solution of ammonium chloride and water. The 2-(dimethylhydroxymethyl)pyrrole was subjected to acid and Lewis acid catalytic macrocyclization giving mixture of calixfnjpyrroles at 65% yield with n = 4-8 as the products with the main product being 1. The present inventors envision this synthesis could be extended to pyrroles with ketone groups in the α-position. β-Octaethylcalixf4Jpyrrole 22. 3,4-Diethylpyrrole (0.369 g, 3 mmol) was dissolved in dry THF (30 mL) under argon and the solution was cooled to -78°C. A MeMgBr solution (3 mL of 1 M solution in diethylether, 3 mmol) was slowly added and stirred at that temperature for 10 minutes. The solution was allowed to warm up to -10°C for 10 minutes and was then cooled to -78°C. At this point, 1.2 molar equivalents of ter/-BuLi was added (3.6 mL of a 1 M molar solution in hexane, 3.6 mmol) and the reaction mixture was kept at -78°C for 30 minutes. The reaction mixture was allowed to warn up to -ICC for 10 minutes and then cooled to -78°C. Acetone (0.29 g, 5 mmol) was added to the solution at that temperature and stirred for 30 min. at -78°C, then allowed slowly warm up to -10°C and kept at that temperature for 1 hour. The reaction was quenched by addition of 1 mL of saturated water solution of ammonium chloride. After evaporation of the organic solvent, the product 21 (Ri = Me, R2 = R3 = Et) was extracted into dichloromethane and isolated by flash chromatography on silica gel with dichloromethane as the mobile phase. Yield 78%. HR MS for Cl 1H19NO calc. 181.146664, found 181.14781. 2-[(Dimethyl)hydroxymethyl)-3,4-diethylpyπole 21 was subjected to acid and Lewis acid catalysed cyclization. The mixture of calixfnjpyrroles was isolated in 45% net yield. The main product has n = 4 22 : (Ci jHi9N) found 652; but a 5% total yield of higher analogues was also obtained. Pyrrole can be substituted for 3,4-diethylpyrrole in this procedure. The present inventors envision that 1 -alkyl pyrrole and 1 -alky 1-3, 4-diethylpyrrole and ketones could be used in a modification of the above procedure: 1 -methy lpyrrole or 1-methyl- 3,4-diethylpyrrole (3 mmol) are dissolved in dry THF (30 mL) under argon and cooled to -78°C. 1.2 molar equivalents of /er/-BuLi are added (3.6 mL of a 1 M molar solution in hexane, 3.6 mmol) and the reaction mixture kept at -78°C for 30 minutes. The reaction is warmed to -10°C for 10 min. and then cooled to -78°C. A general ketone (5 mmol) added and the reaction mixture was stirred for 30 min. at -78°C. The solution is allowed to slowly warm up to -10°C and stirred at that temperature for 1 hour. The reaction is quenched with 1 mL of a saturated water solution of ammonium chloride. After evaporative removal of the organic solvent, the product is extracted to dichloromethane and isolated by flash chromatography on silica gel using dichloromethane as a eluent. The products: 2-[(dialkylhydroxy)methyl]pyrrole derivates were used for the preparation of calixfnjpyrroles using the methodology described above. Example 6 Conversion of Calixfm + njpyrroles to Calixfmjpyridinofnjpyrrole A general synthetic method for the preparation of calixfmjpyridinofnjpyrroles where m and n designate a number of pyridines and pyrroles in the macrocycle, respectively, is unknown to date. Calix[2Jpyridino[2Jpyrroles and calixfl]pyridino[3]pyrroles have been previously synthesized via an organometallic-mediated transformation using zirconium and other metals. However, calixfmjpyridinofnjpyrroles where m > 2 have not been reported. The synthetic approach provided by the present inventors differs from the prior art methods and provides access to a broad variety of calixfmjpyridinofnjpyrroles and calixfmjpyridines. The approach of the present synthetic protocol is based on a non organometallic-mediated pyrrole ring expansion involving one or more of the pyrrole rings contained in a calixfm+njpyrrole macrocycle. Expansion of pyrrole within an existing calixfm+njpyrrole involves the cycloaddition of dichlorocarbene to a particular pyrrolic subunit followed by rearrangement to yield a corresponding pyridine. Dichlorocarbene may be generated under basic or neutral conditions. One or more pyrrolic subunits may react in this procedure specifically, varying the ratio of dichlorocarbene to calixfm+njpyrrole allows control ofthe extent of conversion of pyrrole to pyridine which, in turn, determines the ratio of pyridine subunits to pyrrole subunits in the calixfmjpyridinofnjpyrroles. The present inventors have also discovered a solvent dependence for the conversion of calixfm+njpyrroles to calixfmjpyridinofnjpyrroles. The use of dioxane leads mainly to one and two converted units, while the use of 1,2-dimethoxyethane and prolonged reaction time provides for the full conversion of calixfm+njpyrroles to calixfmjpyridines. Partial conversion of calixfm+njpyrroles to calixfmjpyridinofnjpyrroles is also accomplished by the present methods. Additionally, the present inventors have found that dichlorocarbene generated under neutral conditions (from sodium salts of trichloroacetic acid, for example) gives better yields than generating the dichlorocarbene under basic conditions ( i. e. from the reaction of chloroform with sodium ethoxide). A first method utilizes dichlorocarbene generated under neutral conditions and achieves fully converted calixpyridine product. me.so-Octamethylcalixf4Jpyrrole ( 10 mmol) was dissolved in dry 1 ,2-dimethoxyethane (75 mL). Four molar equivalents of dry sodium trichloroacetate were added per pyrrole ring. The reaction mixture was heated to reflux under an argon atmosphere for 20 hours. After cooling to room temperature, the solution was filtered and the organic solvent was evaporated in vacuo. The resulting residue was redissolved in dichloromethane, treated with charcoal, filtered over celite and evaporated to dryness. The crude product was purified over a short neutral alumina column (dichloromethane eluant). Final separation was achieved by flash chromatography on silica gel with dichloromethane and methanol (0-10 %) as the mobile phase. Yield of fully converted product 27 is 35-50 %. HRMS: For C 32 H 33 N 4 C1 4 [MH + J: calc. 613. 145933, found 613.145490. A second method employs a partial calixf4]pyrrole expansion process to form mixed calixfmjpyridinofnjpyrroles. Mixed systems were obtained using 1-3 molar equivalents of sodium trichloroacetate for each pyrrole ring in the macrocyclic system. The molar ratio of pyrrole/dichlorocarbene allows one to "tune" the number of pyrroles that are converted to pyridines. Using one molar equivalent of sodium trichloroacetate for each pyrrole unit, the main product was calixfljpyridino[3Jpyrrole 23 (76% yield). Using 2 equivalents, the main product was a mixture of the two calix[2jpyridino[2]pyπOle isomers 24 and 25 (83% yield). Using 3 molar equivalents, calix[3jpyridinofl Jpyrrole 26 was obtained (56% yield). These compounds were isolated by flash chromatography and separation was obtained on semipreparative HPLC with dichloromethane-acetonitrile as a solvent and a gradient of acetonitrile from 0-5%. After column and HPLC separation, the following 3-chloropyridine macrocyclic products were characterized by HRMS: Monoconversion: HRMS For C 29 H 35 N 4 C1 calc. 474. 255025, found 474.254879. Bis conversion: HRMS For C 3 oH 34 N 4 Cl 2 calc. 520. 216053; found 520.215789 and 512. 215985. For tris conversion: HRMS For C 3 ιH3 3 N 4 Cl3 calc. 566.177081 , found 566.178902. Mixed molecule systems were further obtained using a third procedure. Dioxane was used as a solvent under the conditions described which resulted in full conversion of the calix f4jpyrrole to calix[4jpyridine, the main products where calixfl]pyridino[3]pyrrole 23, calix[2Jpyridinof2Jpyrrole 24 and 25 and calixf3Jpyridino[l Jpyrrole 26. Conditions: reflux under argon for 15 hours, purification and separation of products as above. Crystals of compound 24 were grown and the structure elucidated by X-ray diffraction. Crystallographic data for 24: Colorless crystals, triclinic, PΪ, Z = 4, in a cell of dimensions: a = 12.544(1), b = 15.406(2), c = 16.770(3)A, α = 63.93(1), β = 82.957(9), γ = 76.958(9)°, V = 2835.0(9)A 3 , pcaic = 1.23 g cm' 3 , F(000) = 1144. 4134 unique reflections, 2783 with F 0 > 4(σ(F 0 )). The final R(F) = 0.113, Rw(F 2 ) = 0.269, goodness of fit = 4.30 for 668 parameters refined on F 2 . Two crystallographically distinct molecules per unit cell. Dihedral angles of rings to root mean square plane through me-ϊø-carbons: pyrrole fl] 18.8°, pyridine f2] 113.2° (23.8°), pyrrole [3] 23.2°, pyridine [4] 67.2° (22.8°), and pyrrole [1] 21.7°, pyridine [2j 69.9° (20.1°), pyrrole [3]161.7° (18.3°), pyridine [4] 67.0° (23.0°). Sodium (1.15 g, 50 mmol) was dissolved in absolute ethanol (25 mL) in a flask fitted with a reflux condenser and a mechanical stirrer and the flask was flushed with argon. Calixf4 Jpyrrole (15 mmol) in chloroform (5 mL) was added and mixture was heated to 55°C for 18 hours. The sodium chloride was filtered off, the residue was treated with charcoal and purified on a short neutral alumina column followed by flash chromatography on silica gel with dichloromethane-methanol (from 0-5%). The main product, was isolated as unreacted starting calix[4Jpyrrole with only 3-9% conversion. 23 24 25 26 27 Example 7 Configurational Isomers of Calixpyrrole The structures of calixf4Jpyrroles described herein are drawn two dimensionally. This representation obscures the fact that calixpyrroles containing more than one asymmetrically- substituted meso-position will be formed as a mixture of configurational isomers. For example, compound 5 (see Example 2), contains four asymmetrically substituted meso-positions and can exist as four different isomers which are chemically distinct from each other and may be separated via HPLC chromatographic techniques. A two dimensional representation is as follows. The four possible configurational isomers of compound 5 are as follows. Therefore, as used herein, all two dimensional representations of calixpyrroles containing more than one asymmetrically substituted weso-position refer to all possible isomers (α,α,α,α), (α,α,α,β), (α,β,α,β) and (α,α,β,β) unless otherwise stated. For calix[5]pyrrole there will also be four possible configurational isomers (α,α,α,α,α), (α,α,α,α,β), (α,β,α,β,α) and (α,α,α,β,β), however for n>5 the number of possible configuarational isomers will increase above four. Example 8 Calixfnjpyrrole Derivatives, Multimers, Conjugates, and Synthesis Thereof Calixfnjpyrrole derivatives, multimers and conjugates have been synthesized. Such molecules possess useful binding properties for ion, chiral substrate, or neutral molecule separation, for salt binding, for acting as sensors for ions or neutral guests and the like. Amido-calixpyrroles 28 and 29 are particularly useful as anion binding agents. Additionally, they serve as model compounds for calixpyrrole modified silica gels wherein the calixpyrrole is linked to the modified silica gel via amide bonds. Mono-β-butylamidocalixf Jpyrrole 28. Compound 12 ('β-hook' acid) (300 mg, 0.62 mmol) was dissolved in DMF (20 mL, dry) under and argon atmosphere. n-Butylamine (45 mg, 0.62 mmol) was added followed by BOP PF6 (327 mg, 0.74 mmol) and triethylamine (125 mg, 1.2 mmol). The reaction mixture was stirred for 48 hours. The DMF was then removed in vacuo and the product purified by column chromatography (SiO 2 , CH C1 : CH3OH 99: 1 eluant) affording compound 28 as a white foam (331 mg, 99%). 'H NMR (250 MHz, CD C1 2 ) δ: 8.80 (s, IH, NH), 7.24 (s, IH, NH), 7.01 (coincident s, 2H, NH), 5.92-5.82 (m, 7H, 6CH py and NH amide ), 5.70-5.69 (IH, d, CH py ), 3.51 (s, 2H, OCH 2 ), 3.29 (q, J = 6.2 Hz, 2H, CH 2 ), 1.66-1.30 (coincident resonances, 28H, 8CH3 meso , 2CH hook )» 0.96 (t, 3H, CH3 hook)- 13 C NMR (62.9 MHz, CD 2 C1 2 ) δ: 173.0, 140.2, 139.7, 139.2, 138.9, 138.4, 138.1 , 137.4, 134.2, 110.7, 106.9, 103.2, 103.2, 103.1, 102.7, 102.5, 101.6, 39.7, 37.1, 35.9, 35.5, 35.4, 35.2, 32.1, 29.2, 29.0, 28.7, 20.5, 14.0. High resolution FABMS calc for C 34 H 47 N 5 0 541.3781 ; found 541.3782 (Δ -0.2 ppm). Mono-meso-butylamidocalixf4Jpyrrole 29. Compound 4 ('meso-hook' acid) (268 mg, 0.43 mmol) was dissolved in DMF (20 mL, dry) under and argon atmosphere. H-Butylamine (31.6 mg, 0.43 mmol) was added followed by BOP PF6 (230 mg, 0.52 mmol) and triethylamine (87 mg, 0.86 mmol). The reaction mixture was stirred for 48 hours. The DMF was then removed in vacuo and the product purified by column chromatography (SiO 2 , CH 2 C1 2 : CH3OH 99:1 eluant) affording compound 29 as a white foam (118 mg, 41%). 'H NMR (250 MHz, CD 2 C1 2 ) δ: 7.26 (s, 2H, NH), 7.17 (s, 2H, NH), 5.93 (s, 2H, CH py ), 5.92 (s, 2H, CH py ), 5.90 (s, 2H, CH py ), 5.89 (s, 2H, CH py ), 5.46 (br.t, IH, NH a mide), 3.17 (q, J = 6.3 Hz, 2H, CH 2 ), 1.87 (br. coincident resonances, 18H, 9CH 2 cyclohexyl) * 1 -4 (br. coincident resoances, 25H, 6CH 2c y C loheχyl + 5CH 2 h ook + CH3 hook), 0.92 (t, J = 7.2 Hz, 3H, CH3). 13 C NMR (62.90 MHz, CD 2 C1 2 ) δ: 172.6, 137.6, 137.2, 137.0, 136.9, 104.0, 103.9, 103.6, 40.0, 39.8, 39.5, 39.4, 38.8, 37.3, 37.0, 36.7, 32.2, 26.4, 25.6, 23.2, 20.9, 20.5, 14.0. High resolution FABMS calc for C 44 H 6 ιN 5 O 675.4876; found 675.4879 (Δ 1.2 ppm). Mono-β-octadecylamidocalix[4]pyrrole Jø.'β-hook' acid 12 (250 mg, 0.51 mmol) was dissolved in DMF (20 mL, dry) under and argon atmosphere. Octadecylamine (139 mg, 0.51 mmol) was added followed by BOP PΕβ (273 mg, 0.61 mmol) and triethylamine (105 mg, 1.03 mmol). The reaction mixture was stirred for 72 hours. The DMF was then removed in vacuo and the product purified by column chromatography (SiO 2 , CH 2 C1 2 : CH3OH 99: 1 eluant) affording the product 30 as a yellow oil (330 mg, 87%). >H NMR (250 MHz, CD 2 C1 2 ) δ: 8.79 (s, IH, NH py ), 7.50 (s, IH, NH py ), 7.03 (s, 2H, NH py ), 5.89-5.80 (coincident multiplets, 7H, 6CH py and NH ami de), 5.67 (d, IH, NH py ), 3.49 (s, 2H, CH 2 CO), 3.26 (q, J = 6.4 Hz, 2H, CH 2 ), 1.68 (m, 12H, 4CH 3 ), 1.49 (m, 12H, 4CH 3 ), 1.29 (m, 32H, CH 2 ), 0.90 (t, J = 6.8 Hz, 3H, CH3). 13 C NMR (62.90 MHz, CD 2 C1 2 ) δ: 173.0, 140.2, 139.7, 139.2, 139.0, 138.4, 138.1, 137.4, 134.3, 1 10.8, 107.0, 103.3, 103.2, 102.7, 102.5, 101.7, 40.1, 37.2, 35.9, 35.50, 35.45, 35.2, 32.4, 30.2, 30.1, 29.8, 29.2, 29.0, 28.7, 27.4, 23.1, 14.3. High resolution FABMS calc for C 48 H 75 N 5 O 737.5972; found 737.5957 (Δ 2.0 ppm). The amide NH group of this receptor contributes to the calix[4]pyrrole anion binding process by providing a fifth hydrogen bond to the bound anion (see Example 10). Molecules such as 30 are designed for insertion into lipid bilayers and are expected to be useful for transport. Propyl bridged β-amidocalix[4 Jpyrrole dimer 31. A calixpyrrole amide dimer compound 31 was formed by coupling 1,3-diaminopropane to the calixpyrrole acid 12. Again, this amide was formed as a DMF complex. This type of receptor is expected to be an effective host for poly-anionic guest species. 1 2 31 DMF Compound 12 (100 mg, 0.206 mmol) and 1,3-diaminopropane (7.62 mg, 0.103 mg) were dissolved in 5 mL DMF under an argon atmosphere. BOP (109 mg, 0.247 mmol) and Et 3 N (41 mg, 0.411 mmol) were added in 10 mL dry DMF, and the solution was stirred overnight. The solvent was then removed under high vacuum and the residue was purified by column chromatography (SiO 2 , CH 2 C1 2 - 1.0% MeOH), affording the dimer 31 as a DMF complex (65 mg, 62%). 'H NMR (500 MHz, CD 2 C1 2 ) δ: 8.63 (s, 2H, NH), 7.93 (DMF), 7.35 (s, 2H, NH), 7.01 (s, 2H, NH), 6.35 (t, 2H, NH amide ), 5.88 - 5.67 (overlapping multiplets, 14H, CH py ), 3.51 (s, 4H, CCH 2 ), 3.25 (m, 4H, CH 2 ), 2.90 (DMF), 2.82 (DMF). High resolution FABMS calc for C 63 H 82 N 10 O 2 1010.6622; found 1010.6630 (Δ -0.7 ppm). This methodology can be extended to produce calixpyrrole trimers, networks of calixpyrroles, linear strings of calixpyrroles, or dendrimer type arrays of calixpyπoles. Additionally all these multimers could be synthesized using the meso-"monohook" acid 4 in a similar synthetic scheme as described herein for the β-substituted molecules. β-Amidobenzo-15-crown-5-meso-octamethylcalixf 4 Jpyrrole 32. 4'-Aminαbenzo-l 5- crown-5 has been coupled to calixf4]pyπole β-monoacid 12 to produce a ditopic receptor which is capable of binding a whole salt (i.e. Na + + Cl-) (FIG. 10) or a zwitterionic amino acid. The calixpyrrole provides an anion binding site and the crown ether a cation binding site, thereby providing a salt complexing agent. meso-Octamefhylcalix[4Jpyrrole β-monoacid 4 (281 mg, 0.58 mmol) and 4'- aminobenzo-15-crown-5 (164 mg, 0.58 mmol) were dissolved in dry DMF (20 mL) and stirred under an argon atmosphere. Benzotriazol-l-yloxytris(dimethylamino)phosphoniurn hexafluorophosphate (BOP PFό) (0.306 g, 0.69 mmol) and triethylamine (1 16 mg) in 20 mL DMF were added and the reaction mixture stirred overnight. The solvent was then removed under high vacuum and the residue purified by column chromatography (SiO 2 , CH 2 C1 2 - 1 to 2% MeOH) affording the amide 32 as a white foam containing alkali metal cations (0.463g). l U NMR (250 MHz, CD 2 Cl 2 ) δ: 8.74 (s, IH, NH py ), 7.76 (s, IH, NH am ), 7.45 (s, IH, ArH), 7.34 (s, IH, NH py ), 7.09 (s, IH, NH py ), 7.02 (s, IH, NH py ), 6.87 (m, 2H, ArH), 4.10 (m, 4H, 2OCH 2 ), 3.87 (m, 4H, 2OCH 2 ), 3.71 (m, 10H, 4OCH 2 + CCH 2 CO), 1.59 (s, 6H, 2CH 3 ), 1.57 (s, 6H, 2CH 3 ), 1.51 (s, 12H, 4CH 3 ). 13 C NMR (62.5 MHz, CD 2 C1 2 ) δ: 171.48, 149.52, 145.96, 140.08, 139.56, 139.18, 138.78, 138.31, 138.14, 137.67, 134.48, 132.73, 1 14.66, 1 12.44, 109.97, 107.19, 106.91, 103.26, 103.13, 103.07, 102.83, 102.31, 101.71, 70.94, 70.40, 69.73, 69.57, 69.04, 37.12, 36.82, 35.45, 35.41, 35.19, 29.17, 28.97, 28.79. FABMS calc for CΦ 1 H57N5O6 751.4309 ; found 751.4310 (Δ -0.2 ppm). Further cation binding agents that may be coupled to calixfnjpyrrole include polyethers; texaphyrins and cryptands for example. Anion binding agents such as sapphyrin or other expanded porphyrins such as rubyrin, rosarin, amethyrin and turcasarin may also be appended to produce polyanionic receptors. Additionally neutral binding groups such as hydrogen bonding arrays may be appended to the calixfnjpyrrole. Mono-meso-ferroceneamidocalix[4]pyrrole 33. The synthesis of new molecular devices designed to sense and report the presence of a particular substrate is an area of analytical chemistry which is attracting intense current interest. Ferrocene is a stable organometallic moiety that has been incorporated into meso- and β-pendant arms of calixf4]pyrrole macrocycles forming molecules which may be used as electrochemical sensors. Compound 4 ('mejo-hook' acid) (325 mg, 0.52 mmol) (see Example 2) was dissolved in DMF (20 mL, dry) under an argon atmosphere. Methylaminofeπocene (112 mg, 0.52 mmol) was added followed by BOP PFβ (278 mg, 0.63 mmol) and triethylamine (106 mg, 1.2 mmol). The reaction mixture was stirred for 48 hours. The DMF was then removed in vacuo and the product purified by column chromatography (SiO 2 , CH 2 C1 2 : CH3OH 99:1 eluant) affording an orange foam 33 (288 mg, 67%). 'H NMR (250 MHz, CD 2 C1 ) δ: 7.24 (s, 2H, NH), 7.16 (s, 2H, NH), 5.93 (d, 4H, CH py ), 5.89 (d, 4H, CH py ), 5.61 (br.t, IH, NH am i d e), 4.16 (s, 7H, FcH + FcCH 2 ), 4.13 (m, 2H, FcH), 4.07 (m, 2H, FcH), 2.05 (t, 2H, CH 2 ), 1.94 (coincident resonances, 16H, 2CH 2 hook + 6CH 2 cyclohexyl, 1-45 (br., 23H, CH 2 hook + CH 3 + 9CH 2 cyclohexyl)- 13 C NMR (62.90 MHz, CD 2 C1 2 ) δ: 172.0, 137.6, 137.1, 137.0, 136.8, 104.0, 103.9, 103.8, 103.6, 85.7, 68.9, 68.5, 68.4, 40.0, 39.8, 39.5, 38.9, 38.8, 37.2, 37.1, 37.0, 36.9, 36.7, 26.4, 25.6, 23.2, 23.1, 20.9. High resolution FABMS calc for C 5 ιH 6 3N 5 O 56 Fe 817.4382; found 817.4364 (Δ 2.2 ppm). Mono-β-ferroceneamidocalix[4Jpyrrole 34. Compound 12 ('β-hook' acid) (250 mg, 0.51 mmol) was dissolved in DMF (20 mL, dry) under an argon atmosphere. Methylaminoferrocene (110 mg, 0.51 mmol) was added followed by BOP PF6 (273 mg, 0.61 mmol) and triethylamine (105 mg, 1.03 mmol). The reaction mixture was stirred for 72 hours. The DMF was then removed in vacuo and the product purified by column chromatography (SiO 2 , CH 2 C1 2 : CH3OH 99:1 eluant) affording an orange foam 34 (294 mg, 84 %). «H NMR (250 MHz, CD 2 C1 2 ) δ: 8.52 (s, IH, NH), 7.38 (s, IH, NH), 7.06 (s, IH, NH), 7.01 (s, IH, NH), 6.06 (s, IH, NH py ), 5.91 (s, 6H, CH py ), 5.74 (IH, d, CH py ), 4.21 (m, 6H, FcH + FcCH 2 ), 4.14 (s, 5H, 5FcH), 3.54 (s, 2H, OCH 2 ), 1.58 (m, 12H, CH 3 ), 1.49 (m, 12H. CH3). 13 C NMR (62.90 MHz, CD 2 Cl 2 ) δ: 172.6, 139.9, 139.4, 139.1, 138.9, 138.4, 138.3, 137.6, 134.3, 110.5, 107.1, 103.3, 103.2, 103.1, 102.9, 102.6, 102.1, 85.7, 68.9, 68.4, 68.3, 39.1, 37.1, 35.9, 35.5, 35.2, 29.2, 29.1, 29.0, 28.8. High resolution FABMS calc for C 41 H 9 N 5 O 56 Fe 683.3287; found 683.3280 (Δ 1.0 ppm) The electrochemical behavior of these macrocycles includes multiple oxidation processes occurring in the calixpyrrole ring. The ferrocene oxidation wave is distinct from these macrocycle oxidations and can be followed using standard electrochemical techniques (cyclic and square wave) (FIG. 11). The ferrocene oxidation wave is perturbed upon addition of anions to the electrochemical solution (FIG. 12) (Table 1). Table 1 Redox potentials (mV) vs Ag/AgCl of the ferrocene moiety in compounds 33 and 34 in the absence and presence of anions (perturbation is shown in brackets) from square wave voltammetry Compound 33 (mV) Compound 34 (mV) Free ligand 453 463 H PO " 401 (-52) 430 (-33) F- 491 (+38) 491 (+28) Cl" 530 (+77) 410 (-53) Larger perturbations are observed for the mew-compound 33 than for the β- compound 34. It has been demonstrated that calixpyrroles can be incoφorated into devices (in this case an electrochemical anion sensor) and operate successfully. meso-Tetrakispolyethyleneglycol-meso-tetramethyl substituted calbf 4 Jpyrrole 35. A polyethylene glycol derivative of calix[4Jpyrrole has been synthesized 35. Reaction of 4-acetylbutyric acid with triethylene glycol monomethyl ether as solvent in the presence of HCl affords a water-soluble triethylene glycol ketone. Pyrrole (48 mg, 0.72 mmol) and the triethylene glycol ketone (200 mg, 0.72 mmol)) were dissolved in methanol (5 mL) and methanesulfonic acid (1 drop) added. The reaction was stirred overnight and the solvent then removed in vacuo affording the water soluble product 35 in 24 % yield. Preparation of amidosaccharide-calix[4]pyrrole conjugate 36. The coupling of protected glucosamine with calixpyrrole β-acid 12 derivative of calixfnjpyrrole produces a water soluble calixpyrrole conjugate. Calixpyrrole β-acid 12 (48.67 mg, 0.1 mmol) was dissolved in dry dichloromethane (50 mL) under argon and diisopropylcarbodiimide (2.5 molar equivalents, 0.25 mmol) was added together with 1 mg of 1 -hydroxybenzotriazole. The solution was stirred for 10 minutes at room temparature under argon. 2-Amino-2-deoxy- 1,3,4,6-tetraacetyl-β-D-glucopyranose hydrobromide salt (2 molar equivalents, 73 mg, 0.2 mmol) was added follwed by 0.3 mL of dry pyridine. The reaction mixture was stirred under argon for 3 days, diluted with dichloromethane (50 mL), washed with 3% hydrochloride acid, water and the organic phase dried over sodium sulfate. The product was isloated on short silica gel column with dichloromethane as eluant. The yield of calix [4 Jpyrrole 36 2-amido-2-deoxy- 1 ,3,4,6-tetra-O-acetyl-β-D-glucopyranose conjugate was 73.4 mg (90%). HR MS for C 44 H5 N5θιo calc. 816.4184, found. 816.4178. Deprotection of the tetraacetylated derivative was performed using standard procedures by dissolution of the protected sugar calix[4Jpyrrole conjugate described above (50 mg) in MeOH, with catalytic amount of sodium methoxide. The solution was stirred for 8 hours at room temperature. The product, calix[4Jpyrrole 2-amido-2- deoxy-β-D-glucopyranose 37 conjugate, was obtained in quantitative yield. For C36H 9Nsθ6 calc 647.36826, found 647.36802. 36 37 A host of hydrophilic substituents can be coupled to β-mono-acid and me_fo-mono-acid; for example, polyethylene glycols, azacrown ethers, crown ethers, phosphate groups, saccarides, modified sugars bearing amino groups, amino alcohols, sulfonyl, chitosan, 2- aminoethylhydrogensulfate, 2-aminoethylphosphoric acid, and the like. Additionally, water solublizing groups, such as sulfonates, phosphates, polyalcohols, polyethers, hydroxyl groups, amino groups, carboxylic acid groups, sulfoxide groups, and other could be incoφorated into the molecule via attachment to the starting ketone and pyrrole before formation of the macrocycle. Calixfnjpyrroles have been shown to bind phosphate groups in the solid state, in solution, and when bound to solid supports as described in Example 10. DNA and RNA, important biological molecules, contain many phosphate groups. A water-soluble calixfnjpyrrole is expected to bind to DNA and RNA. Binding could have the effect of limiting the ability to unwind in the case of DNA, thereby hindering biological functions, or in the case of RNA, could prevent translation. Additionally, oligonucleotides having sequence complementarity to a template molecule may be attached to a calixfnjpyrrole for site- specificity. Uses include inhibition of replication and anti-sense therapy. Example 9 Chiral Calixfnjpyrroles, Calixfmjpyridinofnjpyrroles, and Calixfmjpyridines The present example provides methods for inducing chirality in macrocycles of the present invention, and provides chiral macrocycles for recognition and separation of neutral and anionic chiral molecules. One of skill in this art, in light of the present disclosure, would realize that the methods provided herein for calixpyrroles are applicable to calixfmjpyridinofnjpyrroles, and calixfmjpyridines. A first method is to use chiral molecules as starting materials for the synthesis of calixfnjpyrroles. A second method is to attach optically active (chiral) groups to the molecule via bond formation between functional groups on the calixfnjpyrrole and the chiral auxiliary. A third method is based on transforming achiral substituents into chiral groups by using, for instance, chiral catalysis. Exemplary chiral ketones that can be used to synthesize chiral calixpyrroles include, but are not limited to, chiral ketone containing steroid derivatives, antibiotics e.g. lasalocid, synthetic chiral ketones, and binaphthal groups that possess an inherent chirality. Steroid analogs have the added advantage of forming bowl-shape cavities because ofthe natural bend in the molecule. They have well defined geometries and can act as receptors for anions (e.g. 38). Preparation ofsteroidal calixfnjpyrroles. 4-Cholesten-3-one (38.4 mg, 0.1 mmol) was dissolved in 25 mL of ethanol. Ten molar equivalents of pyrrole was added to the solution. The solution was cooled to 0° C and 10 mg of concentrated HCl in 1 mL of ethanol was added to the reaction mixture which was then stirred for 24 hrs. at room temperature. The solvent was removed in vacuo. The residue was redissolved in dichloromethane (25 mL) and washed with a 3% sodium carbonate solution. The product 38 was purified by flash chromatography on silica gel using a dichloromethane-hexane (1 :1) mobile phase. Yield = 12%. The main product was a calix[4]pyrrole (C3 i H 47 N) 4 Calc'd Mass: 1734. Found: 1734. For calixf 5 Jpyrrole (C3jH 4 7N)5 Calc'd Mass: 2167. Found: 2167. This reaction has been repeated varying the ratio of ketone to pyrrole and also varying the type of acid used. The total yields of calixfnjpyrroles predominantly but not exclusively the n=4 product, were generally in the 10-15% range. Because of their many functional groups, steroid-substituted calixfnjpyrroles containing functional groups capable of being attached to solid supports are likely to exhibit enantioselective separation of, for example, sugars, synthetic oligonucleotides, polypeptides, chiral anions, chiral neutral molecules, or the like. One skilled in the art, in the light of the present disclosure, would appreciate that the resulting species would be amenable to attachment to a solid support. Binaphthal system also possess an inherent chirality. Being so close to the periphery, they could act as "directing groups" and influence binding of neutral and anionic species e.g. 39. A second method for inducing chirality in a molecule is to attach a chiral auxiliary to the main body of the calixfnjpyrrole. This could be done, for example, by forming an amide bond between a carboxycalixfnjpyrrole and a chiral, amino group-containing molecule. This can include, but is not limited to, aminoglycosides, chiral amino alcohols, aminocyclodextrins and amino acids. Many different kinds of functional groups can be coupled to form chemical bonds with a calixfnjpyrrole and would be known in the light ofthe present disclosure. One skilled in the art, in the light of the present disclosure, would appreciate that the resulting species would be ammenable to attachment to a solid support. Chiral Amide β-Substituted calixf4Jpyrrole 40. Monoacid 12 has been coupled to a number of different amines to produce chiral and dimer calixpyrrole species. (R)-(+)-α- methylbenzylamine is a readily available chiral amine. This amine was appended to the calixpyrrole skeleton by conducting an amide coupling reaction between the amine and compound 12 in the presence of BOP and Et 3 N in DMF solution. The amide was isolated as a DMF complex. Chiral calixpyrroles are useful in the chiral separation of racemic mixtures of species such as amino acids. Compound 40 will form a diastereomeric mixture when complexed to fluoride (FIG. 13). Compound 12 (200 mg, 0.41 mmol) and (R)-(+)-α-methylbenzylamine (50 mg, 0.41 mmol) were dissolved in dry DMF (10 mL) and stirred under an argon atmosphere. Benzotriazol-l-yloxytris(dimethylamino)phosphonium hexafluorophosphate (BOP) (0.218 g, 0.494 mmol) and triethylamine (83 mg, 0.82 mmol) in 15 mL DMF were added, and the reaction mixture was stirred overnight. The solvent was then removed under high vacuum and the residue was purified by column chromatography (twice on Si0 2 , CH 2 C1 2 - 2.5% MeOH), affording the chiral amide 40 as a DMF complex (161 mg, 66% yield). l H NMR (250 MHz, CD 2 C1 2 ) δ: 8.76 (s, IH, NH), 7.95 (DMF), 7.33 - 7.28 (m, 5H, PhH), 7.16 (s, IH, NH), 6.96 (2 br s, 2H, NH), 5.86 - 5.65 (overlapping multiplets, 7H, CH py ), 5.09 (m, IH, PhCH), 3.50 (s, 2H, CCH 2 ), 2.90 (DMF), 2.81 (DMF), 1.54 -1.45 (overlapping singlets, 27H, CH 3 ). High resolution FABMS calc for C 3 gH 47 N 5 0 1 589.3781; found 589.3768 (Δ 2.2 ppm). The third method of inducing chirality is reaction of a non-chiral substituent, such as an alkene, to form a chiral side chain and subsequent anti-selective transformation ofthe side chain to a chiral moiety. These types of reactions include, but are not limited to, catalytic asymmetric hydrogenation, asymmetric epoxidation and subsequent ring opening, asymmetric diol formation, asymmetric halogenation and asymmetric amino alcohol formation. Example 10 Calixfnjpyrroles, Calixfmjpyridinofnjpyrroles and Calixfmjpyridines for Solution and Solid Support Binding, and for Separation of Ionic and Neutral Substrates Calixfnjpyrroles and calixfmjpyridinofnjpyrroles for binding and separation possess a pyrrolic hydrogen bonding array capable of coordinating anions via N-H-anion hydrogen bonds. Calixfmjpyridines have a lone pair of electrons on pyridine nitrogens that are capable of donating electron density to a cation. Further, a protonated calixfmjpyridine is capable of electrostatically interacting with an anion. Anion binding properties of calixpyrroles have been studied in solution using 1H NMR titration techniques and are provided herein. Stability constants for octamethylcalix[4Jpyrrole, 1, and tetraspirocyclohexylcalixf4jpyrrole, 2, binding to various anions in dichloromethane were obtained and are provided in Table 2. Table 2: Stability constants for compounds 1 and 2 with anionic substrates 8 Stability Constant (M" 1 ) Calixpyrrole 1 Calixpyrrole 2 Fluoride b 17170 (± 900) 3600 (±395) Chloride 350 (± 5.5) 117 (+4.0) Bromide 10 (+ 0.5) c Iodide < 10 c Dihydrogen phosphate 97 (± 3.9) < 10 Hydrogen sulfate <10 c a Anions were added as 0.1 M CD2CI2 solutions of their tetrabutylammonium salts to receptor to 10 mM receptor solutions in CD2CI2 with concentration changes being accounted for by EQNMR. ^Tetrabutylammonium fluoride was added as the trihydrate. c Undetermined. The data of Table 2 demonstrate that calix[4Jpyrroles show different affinity for different anions in solution, with fluoride being the most tightly bound while iodide is very slightly bound. The present inventors have discovered that varying the substituents on the β-position of a calixpyrrole molecule, however, affects the strength with which a particular anion is held. The data of Table 3 shows the effect of substituting the β-positions ofthe macrocycle with ester 10, methoxy 13, and bromine 14 groups and the effect that these functional groups have on the binding strength of calixf4Jpyrrole to various anions. Additionally compound 11 has been shown to interact with both fluoride and chloride anions in solution. Table 3: Stability constants for compounds 10, 13 and 14 with anionic substrates 3 in CD 2 CI 2 Stability Constant flvH) 10 13 14 Fluoride 1.1 (± 0.2) x l0 3 1.7 (± 0.2) lθ2 2.7 (± 0.4) x l0 4 * Chloride 47 (±1) <10 4.3 (±0.6) x 103 Dibiphosphate c 6.5 (± 0.4) x l0 2 <10 a Anions were added as 0.03 M CD 2 C1 2 solutions of their tetrabutylammonium salts to 3 mM solutions of the receptor in CD 2 C1 with concentration changes being accounted for by EQNMR. In determining the stability constants, the possible effects of ion pairing (if any) were ignored. ^Estimated value. The NH proton resonance broadened considerably during the titration, forcing the frequency ofthe resonance to be noted manually. c Not determined. The attachment of electron-donating substituents (such as methoxy) to the β-position (carbon or C-rim ofthe calixpyrrole) decreases the stability constants of compound 13 relative to compound 2. However attachment of electron- withdrawing substituents (such as bromine) to the carbon-rim has increased the stability constants of compound 14 relative to compound 1. While varying the electron density ofthe calix f4Jpyrrole core increases or decreases the binding affinities towards anions, the present inventors also discovered that groups having a functional moiety at a suitable distance from the macrocycle, and having sufficient flexibility to fold back over the macrocycle also affects the strength of binding as discussed below. The data of Table 4 illustrate the effects of an amide group attached at the meso-position of the calix[4Jpyrrole. Table 4: Stability constants for compounds 28 and 29 with anionic substrates in CD C1 Stability Constants (M' 1 ) Compound 28 (β) Compound 29 (meso) Chloride 405 (± 10) 415 (± 45) Dihydrogen phosphate 80 (± 15) b 62 (± 6) Hydrogen sulfate < 10 < 10 a Anions were added as 0.1 M CD2CI2 solutions of their tetrabutylammonium salts to 10 mM solutions of the receptor in CD2CI2 with concentration changes being accounted for by EQNMR. In determining the stability constants, the possible effects of ion pairing (if any) were ignored. D Estimated value. The NH proton resonance broadened considerably during the titration, forcing the frequency ofthe resonance to be noted manually. As compared to rnejo-tetraspirocyciohexylcalix[4Jpyrrole, 2, the mesø-substituted, amide-substituted caIix[4Jpyrrole 29 shows a marked increase in binding affinities for chloride and phosphate when compared with the values obtained for tetraspirocyclohexyl calix[4Jpyrrole (see Table 2). The amide group contributes to the binding of the anion via an amide-anion hydrogen bond as shown in FIG. 14. This increased binding affinity towards anions was not observed for the β-substituted amide substituted mesø-octamethylcalix[4]pyrrole 28 when compared to the compound 1. A possible reason is that the carboxy "tail" is not long enough to swing around and contribute to the binding ofthe anion. Table 5 Stabiltity constants of the ferrocene-substituted calix{4] pyrroles 33 and 34, measured in CD1CI 2 . Anion 33 meso-ferrocene 34 β-ferrocene H 2 PO 4 40 ± 6 M- i (β-CH) 40 ± 9 M" 1 F 79 ± 7 M- i (β-CH) 1496 ± 370 M- i 206 ± 51 M-' (NH) Cl 202 ± 15 M- 1 444 ± 22 M' 1 The ferrocene modified calixpyrrole binding constants with anions are provided in Table 5. Substrate Binding in Solution. The binding of neutral molecules to caiix[4Jpyrrole in the solid state prompted the present inventors to quantify the binding affinities of calix[4Jpyrrole to a number of hydrogen-binding neutral molecules. Table 6 illustrates the varying degrees of affinity to which calixf4JpyrroIe binds to various hydrogen binding compounds. Table 6: Association Constants for Calix[4]pyrrole, 1, with Neutral Substrates 0 Substrate Added K a (M " ) methanol 12.7 ± 1.0 ethanol 10.7 ± 0.7 benzyl alcohol 9.7 ± 0.7 isopropyl alcohol 7.0 ± 0.4 j-ec-butanol 6.2 ± 0.4 N-formylglycine ethyl ester 13.3 ± 1.0 NN-dimethylformamide 11.3 ± 0.8 NN-dimethylacetamide 9.0 ± 0.9 1,1,3 ,3-tetramethylurea 2.2 ± 0.1 dimethyl sulfoxide 16.2 ± 1.1 1 ,2-dimethy limidazole 5.4 ± 0.3 acetone 2.2 ± 0.2 nitromethane^ — α In benzene-t/g at 298 K. For each titration, the concentration of 1 was held constant (at cα. 4 X 10 " ^ M) as aliquots of the substrate in benzene-.^ (cα. 1 M) were added. " In this instance, the induced shifts in the ΝH proton(s) of 1 were too small (< 0.15 ppm) to afford a reliable K a value. The data in Table 6 demonstrates the ability of calixpyrroles to coordinate to neutral species Separation using Solid-Supported Calix[4Jpyrroles. Following the discovery of the unique anion binding capabilities of calix f4]pyrroles, the present inventors proceeded to attach the "monohook" of Examples 2 and 3 to a silica gel solid support. Calixf4JpyrroIe-substituted silica gel chromatography has demonstrated the ability to separate anionic, neutral hydrogen- binding and aromatic molecular species. In addition to anionic species such as phenyl phosphate, calix [4]pyrrole substituted silica gel has demonstrated the ability to separate polyphosphate-containing compounds such as 5 '-adenosine mono-, di-, and triphosphate under standard, isochratic HPLC conditions at pH = 7. As one might expect, increasing the number of anionic binding sights greatly increases the retention times of these types of molecules. The present approach to anionic and neutral species separation was as follows. The macrocycle, mejo-tris-spirocyclohexyl-monohook-calixf4Jpyrrole, 4, was covalently attached to 5 μ, trimethyl silyl protected, aminopropyl HPLC-grade silica gel obtained from Phase Separations (Νorwalk, CT) to produce Gel M as shown in FIG. 15A. In a similar fashion, β- monohook acid calixf4Jpyrrole 12 was attached to 5 μ, trimethyl silyl protected, aminopropyl HPLC-grade silica gel obtained from Phase Separations (Νorwalk, CT) in the presence of diisopropylcarbodiimide to produce Gel B as shown in FIG. 15B. The attachment of the "monohook" calix f4 Jpyrrole to C-l aminopropyl silica gel is achieved in a greater than 95% yield. The calix [4Jpyrrole columns were packed commercially by Alltech (Deerfield, IL), and various substrates, under differing conditions, were eluted through the columns. All ofthe data provided in the chromatograms herein were obtained at room temperature under isochratic conditions. FIG 16 illustrates the separation of phenyl sulfonate (3), phosphate (4), arsenate (1), and benzoic acid (2) on Gel M . Excellent selectivity is seen among the different anions. FIG. 17 and FIG. 18 provide further examples of separation of biologically-relevant substrates by the calix[4Jpyrrole-substituted silica gel, in this case, 5'-adenosine monophosphate, 5'- adenosine diphosphate and 5'-adenosine triphosphate. The nucleotides described elute in ascending order according to the number of phosphate groups contained within the molecule (i.e. the substrates containing the more anionic groups are retained longer on the column relative to substrates containing fewer anionic groups). Selectivity for carboxylates was also observed for carboxy-substituted benzenes. In two separate experiments, calix[4Jpyrrole substituted silica gel, B, effectively separated benzoic acid, isophthallic acid, and 1,3,5-tricarboxy benzene. In a separate experiment, a calix[4]pyrrole substituted silica gel column effectively separated phthallic acid from a mixture of teraphthallic acid, isophthallic acid and phthallic acid. FIG. 20 and FIG. 21 demonstrate the separation of carboxybenzyl-N-protected amino acids on Gel M and Gel B, respectively. These data illustrate that both hydrophobic contributions and hydrogen bonding interactions are involved in the separation. FIG. 19 illustrates the separation observed between 5 different neutral aromatic compounds: uridine (1), phenol (2), aniline (3), benzene (4), and 4-iodo-nitro benzene (5) on Gel M. In addition to hydrogen-bonding properties, π-π interactions appear to influence separation. It is noteworthy to point out that nucleotides, shown in FIG. 17 and 18, require a substantially stronger buffer system for elution than the nucleoside, uridine (1), shown in FIG. 19, which requires a weak buffer and is eluted almost immediately. Further, the separation of very similar molecules of a class of molecules was carried out. Using a model system analogous to the class of polychlorobiphenyls which are known carcinogens, the separation of polyfluorobiphenyls, a biologically inactive compound, was effected. Demonstrated in FIG. 22, is the separation of four polyfluorobiphenyl compounds; 4-fluorobiphenyl (1), 4,4'-difluorobiphenyl (2), 2,2 , ,3,3 , ,'5,5',6,6'-octafluorobiphenyl (3), and perfluorobiphenyl (4) on Gel M and in FIG. 23 on Gel B. With current interest in antisense oligonucleotide therapy and oligonucleotides as primers and probe molecules, the ability to effectively and efficiently purify usable quantities of these oligomers is of paramount importance. One of the best methods for obtaining large quantities of purified oligonucleotides is high pressure liquid chromatography (HPLC). There are a number of HPLC techniques employed in the purification of oligonucleotides, however, the two most currently used methods are reverse phase- and ion exchange chromatography. These popular methods suffer from practical drawbacks. Purification of medium chain length (12-24) oligonucleotides using reverse phase chromatography must be done using protected substrates. The protecting groups most often used are trityl groups which must be subsequently removed by hydrolysis before use. Additionally, reverse phase chromatography suffers from very long retention times which limits its applicability. Oligonucleotides separated via anion exchange chromatography, while not plagued by the long retention times or additional hydrolysis steps, must be desalted before use. FIG. 24 illustrates a separation of 12 to 18 base mixture of deoxythymidilates (dTi2- 18) on Gel M and FIG. 25 shows the data for Gel B. Additionally mixtures of deoxythymidilates (dT 19-24) were successfully separated on both Gel M and Gel B. The calix[4Jpyrrole-modified silica gel has some immediate advantages over anion exchange columns and reverse phase columns. Wim respect to anion exchange chromatography, the large concentrations of salt that is normally need to effect separation is not needed, thereby removing the mandatory desalting step after chromatography of oligonucleotides before using them in biochemical experiments. The primary advantage that the calixf4Jpyrroles has over reverse phase columns is the higher resolution and shorter retention times. Additionally, reverse phase chromatography of oligonucleotides is done while they are in a "protected" state and must further be hydrolyzed before use. The ability of calix[4]pyrroles to distinguish between specific sequences of nucleotide hexamers was then examined. FIG. 26 provides the data where three different oligonucleotide hexamers, GGGCCC, TCTAGA, and GCATGC, were eluted through Gel M resulting in separation ofthe three hexamers. Mixtures of pesticides (carbofuran, carbendazim, bromacil, bentazon, carboxin and norflurazon) were successfully separated on Gel M. Oligonucleotide therapy has been proposed for a wide range of ailments. These include, but are not limited to sickle cell anemia, hypertension, leukemia, cancer, and AIDS. The basic premise of oligonucleotide therapy is to identify the relevant mRNA or DNA strand that is causing the specific problem and synthesizing a short (< 20 bases) complementary oligonucleotide strand that will bind to the mRNA or DNA, thereby blocking translation or transcription ofthe genetic material. Table 7 illustrates the ability of calix[4Jpyrrole silica gels M and B to separate tetrabutyl ammoniumhalides. TABLE 7: Analysis of Tetrabutylammonium Halides on Calixpyrrole-Substituted Silica Gel Elution Times (minutes) Silica Gel B SilicaGel M Chloride b 17.9 (± 0.1) a 15.2 (± 0.1) a Dihydrogen phosphate b 22.0 (± 0.1 ) a 20.1 (± 0.1 ) a Hydrogen sulfate b 16.2 (± 0.1 ) a 16.2 (± 0.2) a Fluoride b 16.9 (± 0.1 ) a 16.4 (± 0.2) a a Times given for individual elution of tetrabutyl ammonium anions. Separation of mixtures was not possible due to broadening of peaks. b Anions were eluted as 1 mM CH3CN solutions of their tetrabutylammonium salts under the following conditions: Mobile phase, CH3CN; Flow Rate, 0.40 mL/min.; Detection, Conductivity; Column Temperature, 25° C. The present example primarily refers to silica-bound calixpyrrole, however, the solid support attached to the caiixf4Jpyrrole is not limited to silica gel. Further solid supports include, but are not limited to, polystyrene, polyacrylamide, Merrifield resins, glass, sepharose, sephadex, agarose, clays, zeolites, and the like, that can be functionalized to allow the formation of a calixfnjpyrrole bonded to a solid support. Calixfnjpyrroles have been shown to be selective toward a number of different anionic species. In examples described herein, calix[4]pyrrole has shown differing affinities for anionic species including fluoride, chloride, bromide, iodide, phosphates, carboxylates, arsenates and sulfates. Macrocycles ofthe present invention may be used to generate ion selective electrodes in a further use embodiment. Data illustrated in Table 8 for anion selective electrodes synthesized by the present inventors indicate that the selectivity and preferential binding observed in solution and in separation chemistry, described in examples herein, is consistent with anion selective electrode technology. Table 8: Anion Selectivity for a Calixf 4] pyrrole Based Membrane a ' b log KP β tyj Fluoride 0 Chloride -0.7 Bromide -1.0 Iodide +0.4 Hydrogen Sulfate -4.4 D ihy drogen Phosphate -1.7 Hydrogen Sulfite -0.4 Nitrate -0.5 Nitrite -1.8 Acetate -0.9 Benzoate +0.02 Benzene sulfonate -0.5 L-AIanine -1.7 L-Aspartate -1.6 L-Tryptophan -0.5 L-Hystidine -1.6 D-Serine -1.1 DL- Alanine -1.2 DL-Threonine -1.4 Chromotropate -1.0 5-FormyI-2-furansulfonate -0.6 Selectivity coefficients determined relative to fluoride, log KP ot j / j, using the separate solution method. b Membrane compositions: 3% (w/w) calixf4]pyrrole, 22% (w/w) PVC, 75% (w/w) 2- onitrophenyloctyl ether. Internal electrolyte was 0.1 M NaCl and 0.001 M NaF. Methods of making calixfnjpyrrole-ion-selective electrodes include, but are not limited to, the following examples: attaching, or immobilizing a functionalized calixfnjpyrrole to a functionalized polymer and further coating this polymer to an electrode that is sensitive to changes in ionic strength; immobilizing the calixfnjpyrrole in a plasticizers and encasing the mixture in a membrane surrounding an electrode that is sensitive to changes in ionic strength; covalently attaching the calixfnjpyrroles to an electrode that is sensitive to changes in ionic strength; or coating the electrode with a layer of calixfnjpyrroles containing electropolymerizable functional groups, including but not limited to, pyrrole, thiophene or vinyl, and polymerizing the coating on to the electrode. Example 11 Biomedical applications The anion and neutral binding properties of calixfnjpyrrole, calixfmjpyridinofnjpyrrole and calixfmjpyridines allow for a number of useful applications within the biomedical field. Applications include, but are not limited to, drug-delivery systems, anion transport through membranes, selective anion channel formation, dialysis, blood filtration and viral inhibition. Cystic fibrosis is the most common lethal genetic disease among Caucasians, affecting one out of 2,500 infants in the U.S. The disease is characterized by the inability to produce properly-functioning chloride anion channels. The calixfnjpyrroles have been demonstrated herein to bind chloride via hydrogen bonding. An array of calixfnjpyrroles or calixfmjpyridinofnjpyrroles could be designed to span a membrane requiring selective chloride transport. Chloride anion is bound and released appropriately by calix[4]pyrrole as demonstrated in Example 10. Calix f 4 Jpyrrole has also demonstrated the ability to transport anions through an "artificial" dichloromethane membrane in Pressman U-tube experiments. An array of calixfnjpyrroles is expected to transfer anions by "handing off' the anion to the next calixfnjpyrrole. Calixfnjpyrroles can also be used to transport the anion through the membrane by acting as discrete carriers. Referring to the U-tube data, calix[4Jpyrrole has demonstrated the ability to transfer charged molecules through organic membranes. The chloride anion could be transferred through a cell membrane in this way thereby alleviating the problem of chloride transport and ultimately treating the disorder. Another application of anion transport is use of the macrocycles of the present invention as a drug delivery system. Many potential anti-viral agents which display activity ex-vivo are phosphorylated nucleosides. These species are too polar to pass through cell wall membranes and are thus inactive in vivo. Calix [4Jpyrrole has been shown to transport anionic species effectively through "artificial" membranes. The present inventors have synthesized a number of nucleotide conjugates as examples of nucleobase-substituted calixfnjpyrroles. The possibilities of nucleotide substitution include, but are not limited to any of the naturally occurring purine or pyrimidine nucleobases. Additionally, modified versions include any number of purine- or pyrimidine- based synthetic molecules capable of addition to a calixf4]pyrrole core. "Nucleobase," as used herein, means a purine or pyrimidine base, nucleoside (saccharide derivative), nucleotide (saccharide-poly- or mono-phosphate derivative) and natural and synthetic analogues thereof. Attachment of a nucleobase to the calixfnjpyrrole may occur at a functional group of the β-position, a pyrrolic-nitrogen, or rnejo-position of the calixfnjpyrrole. One nucleobase may be attached, however, any number or diversity of nucleobases may be attached to the calixfnjpyrrole. Attachment can occur using methods such as: amide bond formation, Stille coupling, ester formation, or the like. The nucleobase appended calixpyrrole will coordinate to both the phosphate group and nucleoside of the anti-viral drug, forming a hydrophobic complex which may pass through cell wall membranes and decomplex, releasing the drug into the interior ofthe cell. Examples of nucleobase-calixf4]ρyrrole conjugates synthesized by the present inventors are provided as macrocycles 41 and 42. Preparation of nucleobase substituted calixfnjpyrroles 41 and 42. Calixpyrrole monoacid ( 1 mmol) was dissolved in dry dichloromethane (25 mL), (chloroform, acetonitrile, DMF, 1 ,2-dichloroethane can also be used). The solution was activated with 2 molar equivalents (per one acid group) of the activating agent diisopropylcarbodiimide at room temperature in the presence of five molar percent of 1 -hydroxybenzotraizole and 4- dimethylaminopyridine. Two molar equivalents per carboxy unit of an aminoethyl nucleobase derivative (nucleobase protected) were added. The reaction mixture was stirred at room temperature for 24-36 hours. Acetic acid (0.5 mL) was added, followed by multiple washings with a 3% hydrochloric acid solution, water, a 5% solution of sodium hydrogencarbonate and water. After drying over sodium sulfate the product was obtained by crystallization or flash chromatography on silica gel. Removal of the N-protecting group was done using standard procedures. The removal of trityl- and benzyl-protecting groups were achieved using trifluoroacetic acid for trityl and and ammonia in methanol for benzyl protecting groups. Studies have demonstrated that these nucleobase-calix[4jpyrrole conjugates are not only efficient carriers, but selective carriers as well. Using a cytosine-calixf4jpyrrole conjugate to transport a mixture of adenosine monophosphate, cytidine monophosphate, guanidine monophosphate, thymidine monophosphate and uridine monophosphate, a transfer ratio of 5:1 :125:8:7 was observed. The guanidine monophosphate was transported from one aqueous phase to another 10-100 times better across the dichloromethane model membrane as a result of having complementary binding with the cytosine of the cytosine-calix[4]pyrrole conjugate. In addition to being bound to a single nucleobase, calixfnjpyrroles could be attached to oligonucleotides using known technology. Calixfnjpyrrole-oligonucleotide conjugates could then be used to bind such substrates as mRNA or DNA preventing transcription or translation. This mode of binding could be used in antisense therapy, or viral inhibitation. In the case of viral inhibition, the binding site of the virus would be identified and the complementary calix fnjpyrrole-oligonucleo tide conjugates would be synthesized. Calixfnjpyrroles have utility for ex vivo applications as well. Kidney failure is a condition that affects a significant portion of the people in the world due to various ailments. The present example provides for the use of calixfnjpyrroles and calixfmjpyridinofnjpyrroles for dialysis or filtration of ex vivo bodily fluids. Phosphates, chloride, and other toxins contained in the blood or other bodily fluids may be removed by exposure to macrocycles ofthe present invention since said macrocycles have demonstrated the ability to bind anionic and neutral molecules in the solid state, in solution and when attached to a solid support. Further, calixfnjpyrroles can be functionalized to be anion-specific. The present inventors have shown that under chromatographic conditions where anions are bound, proteins and other biomolecules were unhindered in passage through a column, for example. Calixfnjpyrroles could also be used in dialysis. Current dialysis employs an anion gradient to bring anions across a membrane, thereby establishing an equilibrium. Calixfnjpyrroles could be used to bind the anions once they came across the membrane forcing the equilibrium to shift in favor of additional waste anion removal by not allowing an equilibrium to form. The calixfnjpyrroles could either water soluble and partioned on one side of a membrane or bound to a solid support to prevent diffusion into the blood supply. Calixfnjpyrrole could be attached to a biologically-inert solid support and used to filter toxins out ofthe blood. These toxins include, but are not limited to, drug metabolites (for use in drug overdoses) and anionic wastes. Another endogenous material that can function as toxin is urea. Its removal using this approach illustrated could be used in the treatment of gout. Additionally calixpyrroles could be used in imaging. 99m Tc is an high energy form of technetium used in imaging for the detection of tumors. 9 m TcO " or any other anion containing 99m Tc may be coodinated to a calixfnjpyrrole and used as an imaging agent. Example 12 Environmental Remediation Radioactive waste poses a serious problem to the environment. Current technology generates large amounts of secondary solid waste for disposal. Separation and concentration of these wastes is of paramount importance, especially due to long half-lives, and inadequate methods of storage such as sludges or underground tanks of wastewater. Half-life examples for Tc-99, Ni-63, Cs-137, and Sr-90 are 213,000 years, 100 years, 30 years and 28 years, respectively. Certain of the radioactive metallic wastes exist in an oxidized anionic form and are therefore soluble in aqueous solutions. For example, technetium exists as TcO 4 "' and nickel, strontium, and cesium can exist in oxidized anionic forms. Calix [4jpyrrole has demonstrated the ability to bind phenyl arsenate in aqueous media. In addition, FIG. 16 illustrates the ability of calix[4Jpyrrole to separate four different phenyl anions from a mixture thereof. In a column embodiment, solid-supported calixfnjpyrrole could be used to separate out metallic anions from a stream passing through a column. The bound anions could then be eluted from the solid support, and the concentrated eluate is able to be stored in a smaller volume. In a batch embodiment, solid support-bound calixfnjpyrrole could be mixed with a waste solution containing an anion to be removed and the mixture then filtered as a method of extracting the waste anions out of solution. Additionally, a multi-phase extraction system is envisioned where calixfnjpyrrole in a first phase extracts anionic waste from another phase, and the waste then is separated for storage or disposal. The present inventors envision, because of the relatively fast "on-of ' rate that is observed with calix [4Jpyrrole, that the radioactive waste metal could be "washed" off of the solid support for solid support reuse. Current technology uses materials with high binding constants for removal of metallic oxyanion waste. This procedure is efficient for removal ofthe waste from contaminated liquid, however, the waste is converted to solid waste and must still be disposed of. Molecules that can be removed from an environmental source are those ions and molecular species that calixfnjpyrrole will bind as described herein. In particular, for application to inorganic metal oxoanions, arsenate, tungstenate, pertechnetate, borate, or the like, may be removed. Removal of pertechnetate from nuclear waste is a particularly preferred application of the present technology. Types of solid supports for attaching calixfnjpyrrole include, but are not limited to, those supports provided in Example 10. Eutrophication is a serious problem for bodies of water near agricultural lands and urban areas. The accumulation of phosphates and nitrates from fertilizers in lakes, rivers and inland waterways causes toxic algal blooms that poison the water. Algal blooms are also thought to be toxic for humans. Removal of such anions by use of macrocycles of the present invention in a water-treatment plant, for example, would purify the water and provide it for human use and generations ofthe future. Purification of domestic water supplies is an increasing necessity in densely populated areas. The macrocycles of the present invention would be useful in filters for attachment to home water supplies for removal of anionic pollutants such as, but not limited to, fluoride, phosphate and nitrate. All of the compositions and methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the composition, methods and in the steps or in the sequence of steps of the me od described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents which are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept ofthe invention as defined by the appended claims.

Claims: CLAIMS: 1. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; or a calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than zero; the macrocycle noncovalently-complexed to a molecular or anionic species forming a supramolecular ensemble. 2. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; a calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than zero; or a calixfmjpyridine where m is 4, 5, 6, 7, or 8; where the macrocycle attached to a solid support. 3. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8, and m and n are other than zero; or calixfmjpyridine macrocycle where m is 4, 5, 6, 7, or 8 having a first conformation in a solid state when unbound to a molecular or anionic species and having a second conformation in a solid state when bound to a molecular or anionic species. 4. The macrocycle of claim 1, 2, or 3 wherein the macrocycle is a calixfnjpyrrole and the calixfnjpyrrole has structure I: wherein when n is 4, p = q = r = s = 0, R | - R^ are independently substituents as listed in paragraph i) below, and R^ - Rp j are independently substituents as listed in paragraph ii) below; when n is 5, p = 1, q = r = s = 0, R j to R20 are independently substituents as listed in paragraph i) below, and R^ - Rg are independently substituents as listed in paragraph ii) below; when n is 6, p = q = 1, r = s = 0, R j to R24 are independently substituents as listed in paragraph i) below, and R^ - Rp are independently substituents as listed in paragraph ii) below; when n is 7, p = q = r = 1, s = 0, R j to R28 are independently substituents as listed in paragraph i) below, and R^ - RQ are independently substituents as listed in paragraph ii) below; when n is 8, p = q = r = s = 1, R j to R32 are independently substituents as listed in paragraph i) below, and R^ - R j ^ are independently substituents as listed in paragraph ii) below; i) hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, tetrahydrothiapyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester, a site-directing molecule, a catalytic group, a reporter group, a binding agent, or a couple that is coupled to a site- directing molecule, to a catalytic group, to a reporter group, or to a bindingagent; ii) hydrogen, alkyl, aminoalkyl, alkylsulfone, carboxy alkyl, carboxyamidealkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, haloalkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl; or at least two substituents are coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined herein in paragraph i) or ii); wherein odd-numbered R-substituents are other than hydrogen. 5. A β -substituted calixfnjpyrrole macrocycle having structure I: wherein n is 4, 5, 6, 7 or 8; when n is 4, p = q = r = s = 0, R j - R j g are independently substituents as listed in paragraph i) below, and R^ - Rp are independently substituents as listed in paragraph ii) below; when n is 5, p = 1, q = r = s = 0, R j to R20 are independently substituents as listed in paragraph i) below, and R^ - Rg are independently substituents as listed in paragraph ii) below; when n is 6, p = q = 1, r = s = 0, R j to R24 are independently substituents as listed in paragraph i) below, and R^ - Rp are independently substituents as listed in paragraph ii) below; when n is 7, p = q = r = 1, s = 0, R j to R28 are independently substituents as listed in paragraph i) below, and R^ - RQ are independently substituents as listed in paragraph ii) below; when n is 8, p = q = r = s = 1 , R j to R32 are independently substituents as listed in paragraph i) below, and R^ - RH are independently substituents as listed in paragraph ii) below; i) hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, tetrahydrothiapyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester, a site-directing molecule, a catalytic group, a reporter group, a binding agent, or a couple that is coupled to a site- directing molecule, to a catalytic group, to a reporter group, or to a bindingagent; ii) hydrogen, alkyl, aminoalkyl, alkylsulfone, carboxy alkyl, carboxyamidealkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, halo alkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl; or at least two substituents may be coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined herein in paragraph i) or ϋ); and wherein at least one even-numbered R-substituent and all odd-numbered R- substituents are other than hydrogen. 6. A calixfmjpyridinofnjpyrrole macrocycle having structure II: wherein m designates a number of pyridines in the macrocycle and n designates a number of pyrroles in the macrocycle; m+n=4; m is other than 1 or 2; when m is 4, n = 0, p = 1, q = 0, Ri o i to Rl 08 and R20 1 toR2 12 are independently substituents as listed in paragraph i) below, and R30I - R304 are independently substituents as listed in paragraph ii) below; when m is 3, n = 1, p = 0, q = 1, Rl 01 to R1 O8 and R201 to R21 1 are independently substituents as listed in paragraph i) below, and R301 - R304 are independently substituents as listed in paragraph ii) below; i) hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, tetrahydrothiapyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester, a site-directing molecule, a catalytic group, a reporter group, a binding agent, or a couple that is coupled to a site- directing molecule, to a catalytic group, to a reporter group, or to a binding agent; ii) a lone pair of electrons, hydrogen, alkyl, aminoalkyl, alkylsulfone, carboxy alkyl, carboxyamidealkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, halo alkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl; or at least two substituents are coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined herein in paragraph i) or ii); wherein RJQ j -R jQ g are other than hydrogen; wherein when R301 -R304 is other than a lone pair of electrons, Z is 1 ; wherein when R301-R304 is a lone pair of electrons, Z is 0. 7. A calixfmjpyridinofnjpyrrole macrocycle where m and n designate a number of pyridines and pyrroles in the macrocycle, respectively, m and n are other than 0, and m+n=5, 6, 7, or 8; each pyridine or pyrrole α-carbon is bound to another pyridine or pyrrole a- carbon via one non hydrogen-linked sp 3 hybridized mejø-carbon; each sp 3 hybridized meso-carbon is further independently bonded to a halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl or alkyl ester group; to a site-directing molecule; to a catalytic group; to a reporter group; to a binding agent; or to a couple that is coupled to a site-directing molecule, to a catalytic group, to a reporter group, or to a binding agent; each pyridine β carbon, pyrrole β carbon and pyridine γ carbon is independently bonded to a hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester group; to a site-directing molecule; to a catalytic group; to a reporter group; to a binding agent; or to a couple that is coupled to a site-directing molecule; to a catalytic group; to a reporter group, or to a binding agent; each pyridine or pyrrole nitrogen is bound to a lone pair of electrons, hydrogen, alkyl, aminoalkyl, alkylsulfone, carboxy alkyl, carboxyamidealkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, halo alkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl; and or at least one sp 3 hybridized m&so-carbon, pyridine β-carbon, pyrrole β- carbon, pyridine γ carbon, pyrrole nitrogen or pyridine nitrogen is coupled to form a bridged structure to itself or to another sp 3 hybridized meso-carbon, pyridine β-carbon, pyrrole β-carbon, pyridine γ carbon, pyrrole nitrogen, or pyridine nitrogen; and when coupled to form a bridged structure, non-bridged atoms are as defined for an sp 3 hybridized me^σ-carbon, pyridine β-carbon, pyrrole β-carbon, pyridine γ carbon, pyrrole nitrogen, or pyridine nitrogen. A calixfmjpyridine macrocycle having structure III: wherein m is 4, 5, 6, 7 or 8; when m is 4, p = q = r = s = 0, Ri o i to Rl 12 and R20 1 to R2O8 are independently substituents as listed in paragraph i) below, and R30I - R304 are independently substituents as listed in paragraph ii) below; when m is 5, p = 1, q = r = s = 0, Ri oi to Ri 15 and R201 to R210 are independently substituents as listed in paragraph i) below, and R30I - R305 are independently substituents as listed in paragraph ii) below; when m is 6, p = q = 1, r = s = 0, Rl O l to Rl l 8 and R201 to R212 are independently substituents as listed in paragraph i) below, and R30I - R306 are independently substituents as listed in paragraph ii) below; when m is 7, p = q = r = 1, s = 0, Rl O l to Rl21 and R201 ^ R214 are independently substituents as listed in paragraph i) below, and R301 - R307 are independently substituents as listed in paragraph ii) below; when m is 8, p = q = r = s = 1, Ri o i to Rj 24 and R201 to R2 I 6 are independently substituents as listed in paragraph i) below, and R30I - R3O8 are independently substituents as listed in paragraph ii) below; i) hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydrotetrapyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester, a site-directing molecule, a catalytic group, a reporter group, a binding agent, or a couple that is coupled to a site-directing molecule, to a catalytic group, to a reporter group, or to a binding agent; ii) a lone pair of electrons, hydrogen, alkyl, aminoalkyl, alkylsulfone, carboxy alkyl, carboxyamidealkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, halo alkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl; and or at least two substituents are coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined herein in paragraph i) or ii) other than for bridged substituents; wherein R201 - 216 are other than hydrogen; wherein when R301 -R30m is other than a lone pair of electrons, Z is 1 ; and wherein when R301-R30m is a lone pair of electrons, Z is 0. 9. The macrocycle of any one of claims 5-8 wherein the site-directing molecule is an oligonucleotide, an antibody, or a peptide having affinity for a biological receptor. 10. The macrocycle of any one of claims 5-8 wherein the binding agent is a calixfnjpyrrole where n is 4, 5, 6, 7, or 8, a calixfmjpyridinofnjpyrrole where m + n is 4, 5, 6, 7, or 8, and m and n are not 0, a calixarene, a cation-binding functionality, a crown ether, a chelating group, a poφhyrin, or an expanded poφhyrin. 1 1. The macrocycle of any one of claims 5-8 wherein the reporter group is a redox active group, or a fluorescent molecule. 12. The macrocycle of claim 11 wherein the redox active group is ferrocene. 13. The macrocycle of claim 5, 6, 7, or 8 wherein at least one R substituent attached to a meso- carbon is carboxy or carboxyalkyl. 14. The macrocycle of claim 5, 6, 7, or 8 wherein at least one R substituent attached to a non- α, non-meso, macrocyclic carbon. 15. The macrocycle of claim 5, 6, 7, or 8 wherein at least one R substituent attached to a non- α, non-meso, macrocyclic carbon is carboxy, carboxyalkyl, ester, or carboxyamide. 16. The macrocycle of claim 5, 6, 7, or 8 wherein at least one macrocyclic nitrogen is bound to hydrogen. 17. The macrocycle of claim 5 where n is 4, RA- D are each hydrogen, odd-numbered R groups are methyl, one even-numbered R group is (CH2)tCOOH where O≤t≤lO, and even- numbered R groups that are other than (CH2) t COOH are hydrogen. 18. The macrocycle of claim 80 where n is 4, where pyrrolic nitrogens are attached to hydrogen atoms, each of three me_vo-carbons is bridged to itself in a spirocyclohexyl substituent, one mesa-carbon is bonded to a methyl group and to -CH2CH2CH2COOH, and pyrrole β-carbons are bound to hydrogen. 19. A calixfnjpyrrole where n is 5, 6, 7, or 8. 20. A chiral calixfnjpyrrole where n is 4, 5, 6, 7 or 8. 21. A method of making a calixfnjpyrrole where n is 5, 6, 7, or 8, the calixfnjpyrrole having different substituents at a meso-carbon atom, comprising reacting pyrrole and at least two ketone molecules to produce a calixfnjpyrrole having different substitutents at the meso-carbon atoms. 22. The method of claim 21 where the number of different ketone molecules includes acetone and cyclohexanone. 23. A method of making a calixfnjpyrrole where n is 4, 5, 6, 7, or 8, the calixfnjpyrrole having an ester substituent, comprising reacting an ester-functionalized ketone, pyrrole, and a ketone, to produce a calixfnjpyπole having an ester substituent. 24. The method of claim 23 wherein the ester-functionalized ketone is methyl-4- acetylbutyrate, and the ketone is cyclohexanone, acetone, or pentan-3-one. 25. A method of making a calixfnjpyrrole where n is 4, 5, 6, 7, or 8, the calixfnjpyrrole having an acid substituent, comprising reacting an ester-functionalized ketone, pyrrole and a ketone to produce a calixfnjpyrrole ester derivative; and de-esterifying the ester derivative to produce a calixfnjpyrrole monoacid derivative. 26. A method of making a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising activating a calixfnjpyrrole where n is 4, 5, 6, 7, or 8; and reacting the activated calixfnjpyrrole with an electrophile. 27. The method of claim 26 where the activating is deprotonating the calixfnjpyrrole using a Brønsted base, or a Brønsted base in conjunction with a complexing agent. 28. The method of claim 26 where the electrophile is carbon dioxide. 29. The method of claim 26 where the electrophile is etJiylbromoacetate. 30. A method of making a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising reacting a β-substituted pyrrole and a ketone to produce a β-substituted calixfnjpyrrole. 31. The method of claim 30 wherein the β-substituted pyrrole is 3,4-dimethoxypyrrole, and the ketone is cyclohexanone, acetone, or pentan-3-one. 32. A method of making a β-substituted calixfnjpyrrole where n is 4, 5, 6, 7, or 8 comprising producing a calixfnjpyrrole radical where n is 4, 5, 6, 7, or 8; and reacting the radical with a radical acceptor molecule. 33. A method of making a calixfnjpyrrole wherein n is 5, 6, 7, or 8 comprising reacting a pyrrole and a ketone in the presence of a Lewis acid. 34. The method of claim 33 wherein the Lewis acid is a high-valent metal -containing compound or boron trifluoride. 35. The method of claim 33 wherein n is 5. 36. The method of claim 33 wherein n is 6. 37. A method of making a calixfnjpyrrole wherein n is 4, 5, 6, 7, or 8 comprising reacting a pyrrole and a ketone in the presence of a heterogenous catalyst. 38. The method of claim 37 wherein the heterogenous catalyst is montmorrilonite, zeolite, silica gel, alumina, or a cation exchange solid support. 39. The me od of claim 37 wherein n is 5. 40. A method of making a calixfnjpyrrole wherein n is 4, 5, 6, 7, or 8 comprising reacting a pyrrole and a ketone in the presence of an anion template. 41. A method of making a calixfnjpyrrole wherein n is 4, 5, 6, 7, or 8 comprising a) reacting a ketone-functionalized pyrrole and an alkyl or aryl metal to form a pyrrole alcohol, b) condensing the pyrrole alcohol to form a cyclizable pyrrole oligomer; and c) repeating step b) at least n-2 times to form a calixfnjpyrrole. 42. The method of claim 41 wherein the alkyl metal is a Grignard reagent or alkyl lithium. 43. The method of claim 41 wherein n is 5. 44. A method of making a solid-supported calixfnjpyrrole comprising attaching a calixfnjpyrrole having a functionalized group to a solid support, the solid support reactive with the functionalized group, or to a tether-functionalized solid support, the tether reactive with the functionalized group. 45. A method of making a solid-supported calixfmjpyridinofnjpyrrole comprising attaching a calixfmjpyridinofnjpyrrole having a functionalized group to a solid support, the solid support reactive with the functionalized group; or to a tether-functionalized solid support, the tether reactive with the functionalized group. 46. A method of making a solid-supported calixfmjpyridine comprising attaching a calixfmjpyridine having a functionalized group to a solid support, the solid support reactive with the functionalized group, or to a tether-functionalized solid support, the tether reactive with the functionalized group. 47. A calixfnjpyrrole made by the method of claim 21, 23, 25, 26, 27, 30, 32, 33, 37, 40, 41, or 42. 48. A method of forming a complex of a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole and an anion or a neutral molecule, comprising contacting the calixfnjpyrrole or the calixfmjpyridinofnjpyrrole with the anion or neutral molecule under conditions effective to allow the formation ofthe complex. 49. A method for separating a first molecule, a first anion, or first cation from a mixture of molecules, anions or cations, comprising obtaining a calixfnjpyrrole-, a calixfmjpyridinofnjpyrrole- or a calixfmjpyridine- derivatized solid support; and contacting the solid support with the mixture of molecules, anions or cations to separate the first molecule, the first anion or the first cation. 50. The method of claim 49 wherein the solid support is in the form of a chromatography column. 51. The method of claim 49 wherein the solid support is in the form of a capillary electrophoresis tube. 52. The method of claim 49 wherein the contacting is in a batch process. 53. The method of claim 49 wherein the solid support is a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-solid support, the method is for separating a first anion, and the first anion is fluoride, phosphate, a phosphorylated molecule, or nitrate. 54. The method of claim 49 wherein the solid support is a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-solid support, the method is for separating a first molecule, and the first molecule is a nucleotide or an oligonucleotide. 55. The method of claim 49 wherein the method is for separating a first anion or a first cation, and the first anion is an atomic anion or an molecular anion, or the first cation is a atomic cation or a molecular cation. 56. The method of claim 49 wherein the method is for separating a first molecule, and the first molecule is a neutral, cationic or anionic aromatic molecule. 57. The method of claim 49 wherein the solid support is a calixfnjpyrrole solid support and the calixfnjpyrrole has structure I: wherein n is 4, 5, 6, 7 or 8; when n is 4, p = q = r= s = 0, R j - RJ ^ are independently substituents as listed in paragraph i) below, and R^ - RQ are independently substituents as listed in paragraph ii) below; when n is 5, p = 1, q = r = s - 0, R j to R20 are independently substituents as listed in paragraph i) below, and R^ - Rg are independently substituents as listed in paragraph ii) below; when n is 6, p = q = 1, r = s = 0, R j to R24 are independently substituents as listed in paragraph i) below, and R^ - Rp are independently substituents as listed in paragraph ii) below; when n is 7, p = q = r = 1, s =0, R j to R2 are independently substituents as listed in paragraph i) below, and R^ - RQ are independently substituents as listed in paragraph ii) below; when n is 8, p = q = r = s = 1, R j to R32 are independently substituents as listed in paragraph i) below, and R^ - RH ^e independently substituents as listed in paragraph ii) below; i) hydrogen, halide, hydroxyl, alkyl, alkenyl, alkynyl, aryl, alkylaryl, nitro, phospho, formyl, acyl, hydroxyalkyl, alkoxy, hydroxyalkoxy, hydroxyalkenyl, hydroxyalkynyl, saccharide, carboxy, carboxyalkyl, carboxyamide, carboxyamidealkyl, amino, amido, aminoalkyl, phosphoalkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, tetrahydropyran, tetrahydrothiapyran, thioalkyl, haloalkyl, haloalkenyl, haloalkynyl, alkyl ester, a site-directing molecule, a catalytic group, a reporter group, a binding agent, or a couple that is coupled to a site- directing molecule, to a catalytic group, to a reporter group, or to a binding agent; ii) a lone pair of electrons, hydrogen, alkyl, aminoalkyl, alkylsulfone, carboxy alkyl, carboxyamidealkyl, phospho alkyl, alkyl sulfoxide, alkyl sulfone, alkyl sulfide, halo alkyl, aryl, N-oxide, dialkylamino, carbamate, or arylsulfonyl; or at least two substituents are coupled to form a bridged structure, and when coupled to form a bridged structure, nonbridged substituents are as defined herein in paragraph i) or ii); wherein at least one R substituent is attached to the solid support; at least one pyrrole nitrogen is bonded to hydrogen; and odd-numbered R-substituents are other than hydrogen. 58. The method of claim 49 wherein the method is for separating a first anion, the solid support is a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-solid support and the first anion is pertechnetate. 59. The method of claim 49 wherein the method is for separating a first molecule, the solid support is a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-solid support and the first molecule is a polyhalobiphenyl. 60. The method of claim 59 where the polyhalobiphenyl is a polychlorobiphenyl. 61. The method of claim 49 wherein the method is for separating a first anion, the solid support is a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole-solid support, and the first anion is a phosphate anion. 62. A method of transporting a molecular or ionic species through a membrane comprising incoφorating a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole into the membrane; and contacting the membrane with the molecular or ionic species in the presence of a gradient ofthe molecular or ionic species or a counter gradient of a further species. 63. The method of claim 62 wherein the transporting results in the purification of the molecular or ionic species. 64. A method of binding a cation comprising contacting the cation with a calixfnjpyrrole or calixfmjpyridinofnjpyrrole having a cation-binding functionality, or with a calixfmjpyridine. 65. A method of removal of pertechnetate from pertechnetate-containing nuclear waste comprising contacting the waste with a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole to form a calix fnjpyπole or a calixfmjpyridinofnjpyrrole pertechnetate complex; and removing the complex from the waste. 66. A method of removal of an environmental pollutant from an environmental source, comprising contacting the environmental source with a calixfnjpyrrole or a calixfmjpyridinofnjpyrrole to form a calixfnjpyrrole- or a calixfmjpyridinofnjpyrrole- pollutant complex, and removing the complex from the environmental source. 67. A chromatography column comprising a solid support bound to a calixfnjpyrrole where n is 4-8; to a calixfmjpyridinofnjpyrrole where m+n=4-8 and m is not 1 or 2; or to a calixfmjpyridine where m is 4-8. 68. A sensor comprising a solid support bound to a calixfnjpyrrole where n is 4-8; to a calixfmjpyridinofnjpyrrole where m+n=4-8 and m is not 1 or 2; or to a calixfmjpyridine where m is 4-8. 69. Use of a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine in the preparation of a pharmaceutical composition for use in in vivo or ex vivo treatment of body tissues. 70. The use of claim 69 wherein the treatment is ex vivo and is kidney dialysis. 71. The use of claim 69 wherein the treatment is removal of urea for treatment of gout. 72. An electropolymerizable calixfnjpyrrole, calixfmjpyridinofnjpyrrole, or calixfmjpyridine. 73. An anion-, cation-, or neutral molecule-selective electrode comprising a conductive body, a polymer, and a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, or a calixfmjpyridine. 74. The electrode of claim 73 wherein the calixfnjpyrrole, the calixfmjpyridinofnjpyrrole, or the calixfmjpyridine is electropolymerized and forms the conductive body. 75. A method of electrochemical detection of an anion, a cation, or a neutral molecule comprising assembling me anion-, cation-, or neutral molecule-selective electrode of claim 73; contacting the electrode wim a solution of d e anion, the cation, or me neutral molecule; and determining the presence or absence ofthe anion, the cation, or the neutral molecule. 76. The method of claim 66 wherein the pollutant is a polyhalobiphenyl compound, a nitrate compound, or a phosphate compound. 77. A method of making a calixpyridinopyrrole comprising: adding halocarbene to calixpyrrole in a cycloaddition reaction, thereby converting a pyrrole to a pyridine and forming a calixpyridinopyrrole. 78. A method of making a calixpyridine comprising: adding halocarbene to calixpyrrole or to calixpyridinopyrrole in a cycloaddition reaction so as to convert pyrrole to pyridine to form a calixpyridine. 79. The macrocycle of claim 1 wherein the ionic species is an anionic species. 80. A mesø-substituted calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8 wherein the meso-substitution is an oxygen-, sulfur-, or alkenyl-containing substituent; or is a couple to a site-directing molecule, to a binding agent, or to a reporter group. 81. A β-substituted calixfnjyrroie, calixfmjpyridinofnjpyrrole or calixfmjpyridine. 82. The macrocycle of claim 80 wherein the me-fo-substitution is a couple to a site-directing molecule and the site-directing molecule is an oligonucleotide, an antibody, or a peptide having affinity for a biological receptor. 83. The macrocycle of claim 80 wherein the me.sø-substitution is a couple to a binding agent and the binding agent is a calixfnjpyrrole, a calixfmjpyridinofnjpyrrole, a calixarene, a cation- binding functionality, a crown ether, a chelating group, a poφhyrin, or an expanded poφhyrin. 84. The macrocycle of claim 80 wherein the meso-substitution is a couple to a reporter group and the reporter group is a redox active group, or a fluorescent molecule. 85. The macrocycle of claim 84 wherein the reporter group is a redox active group and the redox active group is ferrocene. 86. The macrocycle of claim 80 wherein the meso-substitution is an oxygen-containing substitutent and the oxygen-containing substituent is carboxy or carboxyalkyl. 87. The macrocycle of claim 80 wherein at least one β-carbon has a substituent other than hydrogen. 88. The macrocycle of claim 80 wherein at least one β-carbon has a carboxy, carboxyalkyl, ester, or carboxyamide substituent. 89. The macrocycle of claim 80 wherein at least one macrocyclic nitrogen is bound to hydrogen. 90. A macrocycle selected from the group consisting of compounds 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, and 42. 91. A calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than 1 and 3 or 2 and 2, respectively; or a calixfmjpyridine macrocycle where m is 4, 5, 6, 7, or 8; the macrocycle noncovalently complexed to a molecular or cationic species. AMENDED CLAIMS [received by the International Bureau on 22 September 1997 (22.09.97); original claims 1,2 and 4 amended; new claims 92 and 93 added; remaining claims unchanged (2 pages)] 1. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; or a calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than zero; the macrocycle noncovalently-complexed to an anionic species. 2. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; a calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than zero; or a calixfmjpyridine where m is 4, 5, 6, 7, or 8; where die macrocycle is attached to a solid support. 3. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8, and m and n are other than zero; or calixfmjpyridine macrocycle where m is 4, 5, 6, 7, or 8 having a first conformation in a solid state when unbound to a molecular or amonic species and having a second conformation in a solid state when bound to a molecular or anionic species. 4. The macrocycle of claim 1 , 2, 3, or 92 wherein the macrocycle is a calixfnjpyrrole and the calixfnjpyrrole has structure I: 89. The macrocycle of claim 80 wherein at least one macrocyclic nitrogen is bound to hydrogen. 90. A macrocycle selected from the group consisting of compounds 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 0, 41, and 42. 91. A calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are omer than 1 and 3 or 2 and 2, respectively; or a calixfmjpyridine macrocycle where m is 4, 5, 6, 7, or 8; the macrocycle noncovalently complexed to a molecular or cationic species. 92. A calixfnjpyrrole macrocycle where n is 4, 5, 6, 7, or 8; or a calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than zero; the macrocycle noncovalently-complexed to a molecular species, and the macrocycle in solution or attached to a solid support. 93. A calixfmjpyridinofnjpyrrole macrocycle where m + n is 4, 5, 6, 7, or 8 and m and n are other than zero; the macrocycle noncovalently-complexed to a molecular species.

Inventors: ALLEN WILLIAM E, ANDRIEVSKY ANDREI, BROWN CHRISTOPHER T, GALE PHILIP A, GEBAUER ANDREAS, GENGE JOHN W, KRAL VLADIMIR, LYNCH VINCENT, SANSOM PETRA I, SESSLER JONATHAN L, ALLEN, WILLIAM, E., ANDRIEVSKY, ANDREI, BROWN, CHRISTOPHER, T., GALE, PHILIP, A., GEBAUER, ANDREAS, GENGE, JOHN, W., KRAL, VLADIMIR, LYNCH, VINCENT, SANSOM, PETRA, I., SESSLER, JONATHAN, L.
Applicants: UNIV TEXAS, BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Application year: 1997
Main classification: C07D487
IPC codes: A61K31, A61K48, A61P13, A61P19, B01D15, B01J20, B01J45, C07D471, C07D487, C07D495, C07D498, C07D519, C07F17, C07H15, C07K2, C07K16, G01N30, G21F9