FRUIT RIPENING-RELATED GENES

Abstract: A vector for use in the genetic transformation of strawberry cells comprises a promoter sequence, a regulation sequence and a transcription termination sequence, in which the regulation sequence comprises the coding region, or a fragment of at least 10 bases thereof, of a strawberry protein selected from O-methyl transferase, acyl carrier protein (ACP), elongation factor, auxin-induced gene, cysteine(thiol) proteinase, cellulase, starch phosphorylase, pyruvate decarboxylase, chalcone reductase, protein kinase, auxin-related gene, sucrose transporter, meristem pattern gene, or selected from a strawberry protein with homology to transcribed sequence accession number T45086, transcribed sequence accession number L36159 or transcribed sequence accession number T45902, or selected from a strawberry protein of unknown homology encoded by one of the StrawRipe sequences A to K.

Description: FRUIT RΓPENT G-RELATED GENES This invention relates generally to the modification of a plant phenotype by the regulation of plant gene expression More specifically it relates to the control of fruit ripening by control of one or more than one gene which is known to be implicated in that process BACKGROUND OF THE INVENTION Two principal methods for the control of expression are known These are referred to in the art as "antisense downregulation" and "sense downregulation" or "cosuppression" Both of these methods lead to an inhibition of expression of the target gene Overexpression is achieved by insertion of one or more than one extra copies ofthe selected gene Other lesser used methods involve modification of the genetic control elements, the promoter and control sequences, to achieve greater or lesser expression of an inserted gene In antisense downregulation, a DNA which is complementary to all or part of the target gene is inserted into the genome in reverse orientation and without its translation initiation signal The simplest theory is that such an antisense gene, which is transcribable but not translatable, produces mRNA which is complementary in sequence to mRNA product transcribed from the endogenous gene that antisense mRNA then binds with the naturally produced "sense" mRNA to form a duplex which inhibits translation of the natural mRNA to protein It is not necessary that the inserted antisense gene be equal in length to the endogenous gene sequence a fragment is sufficient The size of the fragment does not appear to be particularly important Fragments as small as 40 or so nucleotides have been reported to be effective Generally somewhere in the region of 50 nucleotides is accepted as sufficient to obtain the inhibitory effect However, it has to be said that fewer nucleotides may very well work a greater number, up to the equivalent of full length, will certainly work It is usual simply to use a fragment length for which there is a convenient restriction enzyme cleavage site somewhere downstream of fifty nucleotides The fact that only a fragment ofthe gene is required means that not all of the gene need be sequenced It also means that commonly a cDNA will suffice, obviating the need to isolate the full genomic sequence The antisense fragment does not have to be precisely the same as the endogenous complementary strand of the target gene There simply has to be sufficient sequence similarity to achieve inhibition of the target gene This is an important feature of antisense technology as it permits the use of a sequence which has been derived from one plant species to be effective in another and obviates the need to construct antisense vectors for each individual species of interest Although sequences isolated from one species may be effective in another, it is not infrequent to find exceptions where the degree of sequence similarity between one species and the other is insufficient for the effect to be obtained. In such cases, it may be necessary to isolate the species-specific homologue. Antisense downregulation technology is well-established in the art It is the subject of several textbooks and many hundreds of journal publications The principal patent reference is European Patent No. 240,208 in the name of Calgene Ine There is no reason to doubt the operability of antisense technology It is well-established, used routinely in laboratories around the world and products in which it is used are on the market Both overexpression and downregulation are achieved by "sense" technology If a full length copy of the target gene is inserted into the genome then a range of phenotypes is obtained, some overexpressing the target gene, some underexpressing A population of plants produced by this method may then be screened and individual phenotypes isolated As with antisense, the inserted sequence is lacking in a translation initiation signal. Another similarity with antisense is that the inserted sequence need not be a full length copy Indeed, it has been found that the distribution of over-and under-expressing phenotypes is skewed in favour of underexpression and this is advantageous when gene inhibition is the desired effect. For overexpression, it is preferable that the inserted copy gene retain its translation initiation codon. The principal patent reference on cosuppression is European Patent 465,572 in the name of DNA Plant Technology Inc. There is no reason to doubt the operability of sense/co- suppression technology. It is well established, used routinely in laboratories around the world and products in which it is used are on the market Sense and antisense gene regulation is reviewed by Bird and Ray in Biotechnology and Genetic Engineering Reviews 9 : 207-227 ( 1991 ). The use of these techniques to control selected genes in tomato has been described by Gray et al., Plant Molecular Biology, J9 69-87 (1992). Gene control by any ofthe methods described requires insertion of the sense or antisense sequence, with appropriate promoters and termination sequences containing polyadenylation signals, into the genome ofthe target plant species by transformation, follow by regeneration ofthe transformants into whole plants. It is probably fair to say that transformation methods exist for most plant species or can be obtained by adaptation of available methods For dicotyledonous plants the most widely used method is Agrobacterium- mediated transformation. This is the best known, most widely studied and, therefore, best understood of all transformation methods The rhizobacterium Agrobacterium tumefaciens, or the related Agrob cterium rhizogenes, contain certain plasmids which, in nature, cause the formation of disease symptoms, crown gall or hairy root tumours, in plants which are infected by the bacterium. Part of the mechanism employed by Agrobacterium in pathogenesis is that a section of plasmid DNA which is bounded by right and left border regions is transferred stably into the genome of the infected plant. Therefore, if foreign DNA is inserted into the so-called "transfer" region (T-region) in substitution for the genes normally present therein, that foreign gene will be transferred into the plant genome. There are many hundreds of references in the journal literature, in textbooks and in patents and the methodology is well-established. Agrobacterium-mediated transformation ofthe cultivated strawberry (Fragaria x ahanassa Duch. is described in Plant Science, 69, 79-94 (1990). The effectiveness oi Agrobacterium is restricted to the host range of the micro¬ organism and is thus restricted more or less to dicotyledonous plant species. In general monocotyledonous species, which include the important cereal crops, are not amenable to transformation by the Agrobacterium method. Various methods for the direct insertion of DNA into the nucleus of monocotyledon cells are known. In the ballistic method, microparticles of dense material, usually gold or tungsten, are fired at high velocity at the target cells where they penetrate the cells, opening an aperture in the cell wall through which DNA may enter. The DNA may be coated on to the microparticles or may be added to the culture medium. In microinjection, the DNA is inserted by injection into individual cells via an ultrafine hollow needle. Another method, applicable to both monocotyledons and dicotyledons, involves creating a suspension of the target cells in a liquid, adding microscopic needle-like material, such as silicon carbide or silicon nitride " whiskers", and agitating so that the cells and whiskers collide and DNA present in the liquid enters the cell In summary, then, the requirements for both sense and antisense technology are known and the methods by which the required sequences may be introduced are known. What remains, then is to identify genes whose regulation will be expected to have a desired effect, isolate them or isolate a fragment of sufficiently effective length, construct a chimeric gene in which the effective fragment is inserted between promoter and termination signals, and insert the construct into cells ofthe target plant species by transformation. Whole plants may then be regenerated from the transformed cells. This invention is concerned with the control of ripening in fruit, and the particular interest here is in strawberries. The interest in controlling the ripening process is to improve the flavour and/or texture of the fruit, both characters being largely affected by the ripening process. Sugars are the most important soluble component of the flavour. Some 99% of the soluble sugars in strawberry are accounted for by sucrose, glucose and fructose, the amount of these sugars being affected by the season but their relative proportions are largely unaffected. There is little information in the literature on the metabolic pathways involved in the synthesis of sugars in strawberry. It is known, however that sugars are synthesised during the ripening ofthe fruit. The changes in gene expression during strawberry fruit ripening and their regulation by auxin have been described in Planta 194: 62-68 (1994) OBJECT OF THE INVENTION An object of the present invention is to provide DNA sequences enabling the construction of vectors suitable for genetic transformation of strawberry plants, with a view to control of the ripening process in strawberry fruit. SUMMARY OF THE INVENTION According to the present invention there is provided a vector for use in the genetic transformation of strawberry cells, comprising a promoter sequence, a regulation sequence and a transcription termination sequence, in which the regulation sequence comprises the coding region, or a fragment of at least 10 bases thereof, of a strawberry protein selected from O-methyl transferase, acyl carrier protein (ACP), elongation factor, auxin-induced gene, cysteine(thiol) proteinase, cellulase, starch phosphorylase, pyruvate decarboxylase, chalcone reductase, protein kinase, auxin- related gene, sucrose transporter, meristem pattern gene, or selected from a strawberry protein with homology to transcribed sequence accession number T45086, transcribed sequence accession number L36159 or transcribed sequence accession number T45902, or selected from a strawberry protein of unknown homology encoded by one of the StrawRipe sequences A to K. The gene regulation sequence may be in the same or antisense orientation as the endogenous target gene. It may also be of partial or full sequence length. The invention further contemplates the overexpression of one or more of the genes by inserting into the strawberry genome one or more than one extra copy thereof. The invention also provides a gene regulation sequence which comprises the coding region, or a fragment of at least 10 bases thereof, of a strawberry protein selected from O-methyl transferase, acyl carrier protein (ACP), elongation factor, auxin-induced gene, cysteine(thiol) proteinase, cellulase, starch phosphorylase, pyruvate decarboxylase, chalcone reductase, protein kinase, auxin-related gene, sucrose transporter, meristem pattern gene, or selected from a strawberry protein with homology to transcribed sequence accession number T45086, transcribed sequence accession number L36159 or transcribed sequence accession number T45902, or selected from a strawberry protein of unknown homology encoded by one of the StrawRipe sequences A to K The sequences of this invention can also be used as probes for isolation of similar sequences from the strawberry genome The invention also provides a strawberry plant and propagating material thereof which contains a vector of this invention. Further according to the invention, there is provided a method for altering the phenotype of strawberry plants, with the aim of controlling the ripening of strawberry fruit, comprising inserting into the genome ofthe cell of a strawberry plant a gene regulation vector of this invention In this way, the invention further provides genetically modified strawberry plants, propagation material and strawberry fruit PREFERRED EMBODIMENTS In the present invention, the regulation sequence comprises the coding region, or a fragment of at least 10 bases thereof, of a strawberry protein The strawberry protein is selected from O-methyl transferase, acyl carrier protein (ACP), elongation factor, auxin-induced gene, cysteine(thiol) proteinase, cellulase, starch phosphorylase, pyruvate decarboxylase, chalcone reductase, protein kinase, auxin-related gene, sucrose transpoπer, meristem pattern gene, or selected from a strawberry protein with homology to transcribed sequence accession number T45086, transcribed sequence accession number L36159 or transcribed sequence accession number T45902, or selected from a strawberry protein of unknown homology encoded by one of the StrawRipe sequences A to K Examples of suitable regulation sequences are SEQ LD NO 1 to SEQ LD NO.27., also referred to herein as Sequences 1 to 27 Related sequences taken from the priority documents of the present PCT application are given in SEQ LD NO 28 to SEQ LD NO 38 The gene regulation sequences of the invention may be synthesised from the sequence information given or may be isolated from a library To assist isolation Zeneca Limited have deposited with the National Collection of Industrial & Marine Bacteria, St Machar Drive, Aberdeen, UK, a cDNA library of strawberry ripening genes The library was deposited on 15th November 1994 under the Budapest Treaty and has the Accession Number NCIMB 40690 Thus, this invention is based on the identification of genes which encode proteins implicated in strawberry ripening-related processes DNA sequences which encode these proteins have been cloned and some have been characterised The DNA sequences may be used to modify plants with the goal of modifying the ripening characteristics of fruit. By virtue of this invention strawberry plants can be generated which, amongst other phenotypic modifications, may have one or more of the following fruit characteristics improved resistance to damage during harvest, packaging and transportation due to slowing of the ripening and over-ripening processes, longer shelf life and better storage characteristics due to reduced activity of degradative pathways (e g cell wall hydrolysis), improved processing characteristics due to changed activity of proteins/enzymes contributing to factors such as viscosity, solids, pH, elasticity, improved flavour and aroma at the point of sale due to modification ofthe sugar/acid balance and other flavour and aroma components responsible for characteristics ofthe ripe fruit, modified colour due to changes in activity of enzymes involved in the pathways of pigment biosynthesis (e.g lycopene, β-carotene, chalcones and anthocyanins), increased resistance to post-harvest pathogens such as fungi The activity of the ripening-related proteins may be either increased or reduced depending on the characteristics desired for the modified plant part (fruit, leaf, flower, etc) The levels of protein may be increased; for example, by incoφoration of additional genes The additional genes may be designed to give either the same or different spatial and temporal patterns of expression in the fruit "Antisense" or "partial sense" or other techniques may be used to reduce the expression of ripening- related protein The activity of each ripening-related protein or enzyme may be modified either individually or in combination with modification ofthe activity of one or more other ripening-related proteins/enzymes In addition, the activities of the πpening-related proteins/enzymes may be modified in combination with modification of the activity of other enzymes involved in fruit ripening or related processes DNA constructs according to the invention may comprise a base sequence at least 10 bases (preferably at least 35 bases) in length for transcription into RNA There is no theoretical upper limit to the base sequence - it may be as long as the relevant mRNA produced by the cell - but for convenience it will generally be found suitable to use sequences between 100 and 1000 bases in length. The preparation of such constructs is described in more detail below. As a source of the DNA base sequence for transcription, a suitable cDNA or genomic DNA or synthetic polynucleotide may be used. The isolation of suitable ripening-related sequences is described above; it is convenient to use DNA sequences derived from the ripening-related clones deposited at NCLMB in Aberdeen. Sequences coding for the whole, or substantially the whole, ofthe appropriate ripening-related protein may thus be obtained. Suitable lengths of this DNA sequence may be cut out for use by means of restriction enzymes. When using genomic DNA as the source of a base sequence for transcription it is possible to use either intron or exon regions or a combination of both. In a variation ofthe vector of this invention the regulation sequence varies from Sequences 1 to 27 but retains sufficient similarity to be effective in gene regulation. Thus, the regulatory gene may be a homologue of a gene of Sequence 1 to 27 which has been obtained from a strawberry plant. To obtain constructs suitable for expression of the appropriate ripening-related sequence in plant cells, the cDNA sequence as found in one of the strawberry plasmids or the gene sequence as found in the chromosome ofthe strawberry plant may be used. Recombinant DNA constructs may be made using standard techniques. For example, the DNA sequence for transcription may be obtained by treating a vector containing said sequence with restriction enzymes to cut out the appropriate segment. The DNA sequence for transcription may also be generated by annealing and ligating synthetic oligonucleotides or by using synthetic oligonucleotides in a polymerase chain reaction (PCR) to give suitable restriction sites at each end. The DNA sequence is then cloned into a vector containing upstream promoter and downstream terminator sequences. If antisense DNA is required, the cloning is carried out so that the cut DNA sequence is inverted with respect to its orientation in the strand from which it was cut. Promoters suitable for use in constructs of the invention may be any suitable promoters which are known to be effective in driving expression of foreign genes in plants, for example the promoters may be those which are isolatable from the genomic version of the cDNAs of the invention. In a construct expressing antisense RNA, the strand that was formerly the template strand becomes the coding strand, and vice versa. The construct will thus encode RNA in a base sequence which is complementary to part or all of the sequence of the ripening-related RNA. Thus the two RNA strands are complementary not only in their base sequence but also in their orientations (5' to 3') In a construct expressing sense RNA, the template and coding strands retain the assignments and orientations ofthe original plant gene. Constructs expressing sense RNA encode RNA with a base sequence which is homologous to part or all of the sequence ofthe mRNA. In constructs which express the functional ripening-related protein, the whole ofthe coding region ofthe gene is linked to transcriptional control sequences capable of expression in plants. For example, constructs according to the present invention may be made as follows. A suitable vector containing the desired base sequence for transcription is treated with restriction enzymes to cut the sequence out. The DNA strand so obtained is cloned (if desired, in reverse orientation) into a second vector containing the desired promoter sequence and the desired terminator sequence. Suitable promoters include the 35S cauliflower mosaic virus promoter, the polyubiquitin promoter and the tomato polygalacturonase gene promoter sequence (Bird et al, 1988, Plant Molecular Biology, 1 1 :651 -662) or other developmentally regulated fruit promoters. Suitable terminator sequences include that of the Agrobacterium tumefaciens nopaline synthase gene (the nos 3' end). The transcriptional initiation region (or promoter) operative in plants may be a constitutive promoter (such as the 35S cauliflower mosaic virus promoter) or an inducible or developmentally regulated promoter (such as fruit-specific promoters), as circumstances require. For example, it may be desirable to modify ripening-related protein activity only during fruit development and/or ripening. Use of a constitutive promoter will tend to affect ripening-related protein levels and functions in all pans of the plant, while use of a tissue specific promoter allows more selective control of gene expression and affected functions. Thus in applying the invention it may be found convenient to use a promoter that will give expression during fruit development and/or ripening. Thus the antisense or sense RNA is produced only in the organ in which its action is required and/or only at the time required. Fruit development and/or ripening-specific promoters that could be used include the ripening-enhanced polygacturonase promoter (PCT WO 92/08798), the E8 promoter (Diekman & Fischer, 1988, EMBO, 7:3315-3320), the fruit specific 2AII promoter (Pear et al, 1989, Plant Molecular Biology, 13:639-651), the histidine decarboxylase promoter (HDC, Sibia) and the phytoene synthase promoter. Ripening-related protein or enzyme activity (and hence ripening-related processes and fruit ripening characteristics) may be modified to a greater or lesser extent by controlling the degree of the appropriate ripening-related protein's sense or antisense mRNA production in the plant cells. This may be done by suitable choice of promoter sequences, or by selecting the number of copies or the site of integration of the DNA sequences that are introduced into the plant genome. For example, the DNA construct may include more than one DNA sequence encoding the ripening-related protein or more than one recombinant construct may be transformed into each plant cell. The activity of each ripening-related protein may be separately modified by transformation with a suitable DNA construct comprising a ripening-related sequence In addition, the activity of two or more ripening-related proteins may be simultaneously modified by transforming a cell with two or more separate constructs. Alternatively, a plant cell may be transformed with a single DNA construct comprising both a first ripening-related sequence and a second ripening-related sequence. It is also possible to modify the activity ofthe ripening-related protein(s) while also modifying the activity of one or more other enzymes. The other enzymes may be involved in cell metabolism or in fruit development and ripening. Cell wall metabolising enzymes that may be modified in combination with a ripening-related protein include but are not limited to: pectin esterase, polygalacturonase, β-gal actanase, ,β-glucanase. Other enzymes involved in fruit development and ripening that may be modified in combination with a ripening-related protein include but are not limited to: ethylene biosynthetic enzymes, carotenoid biosynthetic enzymes including phytoene synthase, carbohydrate metabolism enzymes including invertase. Several methods are available for modification ofthe activity ofthe ripening-related protein(s) in combination with other enzymes. For example, a first plant may be individually transformed with a ripening-related gene construct and then crossed with a second plant which has been individually transformed with a construct encoding another enzyme. As a further example, plants may be either consecutively or co-transformed with ripening-related constructs and with appropriate constructs for modification ofthe activity ofthe other enzyme(s). An alternative example is plant transformation with a ripening-related construct which itself contains an additional gene for modification of the activity of the other enzyme(s). The ripening-related gene constructs may contain sequences of DNA for regulation of the expression of the other enzyme(s) located adjacent to the ripening-related sequences. These additional sequences may be in either sense or antisense orientation as described in PCT/WO 93/23551 (single construct having distinct DNA regions homologous to different target genes) By using such methods, the benefits of modifying the activity of the ripening-related proteins may be combined with the benefits of modifying the activity of other enzymes. A DNA construct ofthe invention is transformed into a target plant cell The target plant cell may be part of a whole plant or may be an isolated cell or part of a tissue which may be regenerated into a whole plant. For any particular plant cell, the ripening-related sequence used in the transformation construct may be derived from the same plant species, or may be derived from any other plant species (as there will be sufficient sequence similarity to allow modification of related isoenzyme gene expression). Transgenic plants and their progeny may be used in standard breeding programmes, resulting in improved plant lines having the desired characteristics For example, fruit-bearing plants expressing a ripening-related construct according to the invention may be incoφorated into a breeding programme to alter fruit-ripening characteristics and/or fruit quality Such altered fruit may be easily derived from elite lines which already possess a range of advantageous traits after a substantial breeding programme: these elite lines may be further improved by modifying the expression of a single targeted ripening-related protein enzyme to give the fruit a specific desired property. By transforming plants with DNA constructs according to the invention, it is possible to produce plants having an altered (increased or reduced) level of expression of one or more ripening-related proteins, resulting from the presence in the plant genome of DNA capable of generating sense or antisense RNA homologous or complementary to the RNA that generates such ripening-related proteins For fruit-bearing plants, fruit may be obtained by growing and cropping using conventional methods Seeds may be obtained from such fruit by conventional methods (for example, tomato seeds are separated from the pulp of the ripe fruit and dried, following which they may be stored for one or more seasons) Fertile seed derived from the genetically modified fruit may be grown to produce further similar modified plants and fruit. The fruit derived from genetically modified plants and their progeny may be sold for immediate consumption, raw or cooked, or processed by canning or conversion to soup, sauce or paste. Equally, they may be used to provide seeds according to the invention. The genetically modified plants (transformed plants and their progeny) may be heterozygous for the ripening-related DNA constructs. The seeds obtained from self fertilisation of such plants are a population in which the DNA constructs behave like single Mendelian genes and are distributed according to Mendelian principles: e.g., where such a plant contains only one copy ofthe construct, 25% of the seeds contain two copies ofthe construct, 50% contain one copy and 25% contain no copy at all. Thus not all the offspring of selfed plants produce fruit and seeds according to the present invention, and those which do may themselves be either heterozygous or homozygous for the defining trait. It is convenient to maintain a stock of seed which is homozygous for the ripening-related DNA construct. All crosses of such seed stock will contain at least one copy of the construct, and self-fertilized progeny will contain two copies, i.e. be homozygous in respect ofthe character. Such homozygous seed stock may be conventionally used as one parent in Fl crosses to produce heterozygous seed for marketing. Such seed, and fruit derived from it, form further aspects of our invention. We further provide a method of producing FI hybrid plants expressing a ripening-related DNA sequence which comprises crossing two parent lines, at least one of which is homozygous for a ripening-related DNA construct. A process of producing Fl hybrid seed comprises producing a plant capable of bearing genetically modified fruit homozygous for a ripening-related DNA construct, crossing such a plant with a second homozygous variety, and recovering Fl hybrid seed. It is possible according to our invention to transform two or more plants with different ripening-related DNA constructs and to cross the progeny of the resulting lines, so as to obtain seed of plants which contain two or more constructs leading to reduced expression of two or more fruit-ripening-related proteins EXAMPLES OF THE INVENTION The invention will now be described, by way of illustration, by the following Examples. In the Examples, reference is made to Figure 1 THE DRAWING Figure 1 is a diagrammatic map of plasmid pBINCEL EXAMPLE 1 Construction of a cDNA library of ripening genes 1 1 Isolation of messenger RNA Total RNA was isolated from ripe fruit tissue (the receptacle with the achenes removed) of strawberry (Fragaria x ananassa Duch cv Brighton) as described by Manning K Analytical Biochemistry 195. 45-50 (1991) Messenger RNA was isolated from total RNA by oligo(dT)-cellulose chromatography according to Bantle et al , Analytical Biochemistry 72, 413-427 (1976) 1.2 Synthesis of cDN A The first and second strands o the cDNAs were synthesised from messenger RNAs using a commercial cDNA synthesis kit (RPN 1256Y Amersham Life Sciences. Amersham, Bucks , UK), priming the first strand cDNA synthesis with oligo-dT 1 3 Cloning into vector Double stranded cDNAs were cloned into the λgtlO vector using the BRL cloning system (8287SA. Bethseda Research Laboratories, Paisley, Renfrewshire, UK) essentially as follows Internal EcoRI sites of the cDNAs were methylated using EcoRI methylase The DNA termini were repaired with T4 DNA polymerase and phosphorylated EcoRI linkers ligated to the cDNA with T4 ligase Excess linkers were digested and removed by column chromatography on DEAE-Sephadex The purified double stranded cDNAs with EcoRI termini were ligated into λgtlO vector DNA digested with EcoRI and dephosphorylated Vector DNA was then packaged using an in vitro packaging extract (Promega Coφoration, Southampton, UK) Recombinant bacteriophage were mixed with plating bacteria (E. coli C600 hflA 150) as described in the BRL protocol to determine titre, for library screening and subsequent amplification 1 4 Screening of the cDNA library from ripe strawberry The unamplified cDNA library from ripe strawberry was differentially screened using cDNA from fruit receptacle tissue at the ripe and white stages of ripeness A proportion of the library was plated at low density and duplicate plaque lifts made on to Hybond N nylon filters (Amersham) according to the manufacturer's instructions One filter was hybridised to ripe cDNA from white fruit and the duplicate filter hybridised to ripe cDNA. Hybridisations were at high stringency using digoxigenin as a non-radioactive label (Boehringer Mannheim, Lewes, Sussex, UK) Plaques hybridising preferentially to ripe cDNA were picked and replated at low density for a second round of selection by differential screening Single plaques from the second screening were picked and numbered as ripening-enhanced clones 1.5 Characterisation of the ripe cDNA library and ripening-enhanced clones The ripe cDNA library was prepared with an efficiency of 3.03x 106 plaque-forming units per microgram of cDN A. The size of the cDNA inserts in this library ranged from approximately 0.24 to 6 kbp with a mean insert size of approximately 1 4 kbp. From the 1343 plaques used in the first screen, 83 putative ripening clones were obtained. Of these, 48 were pure clones with single inserts, the remainder being impure and having multiple inserts. The 48 clones with single inserts were partially sequenced using the DyeDeoxy (Trade Mark) Terminator Cycle Sequencing Kit (Applied Biosystems, Warrington, Cheshire, UK) with forward and reverse primers specific for the λgtlO vector. Improved sequence data were obtained for clones with multiple inserts and clones with single inserts that did not produce good sequence data by subcloning into the phagemid vector pBK-CMV (Stratagene) vector for sequencing. From the sequenced clones, the following twenty-seven ripening-related clones were seleαed. Comparison of these sequences with sequences in the EMBL database using GCG ("Winconsin") software has identified homologies for the clones of sequences 1 to 16 listed in the following table 1. Sequence ID Homology /Identity Clone number NO 1 O-methyl transferase 1 2 acyl carrier protein (ACP) 3 3 elongation factor 33a 4 auxin-induced gene 33b 5 cy stein e( thiol) proteinase 93 c 6 cellulase 97 7 starch phosphorylase 6ab 8 pyruvate decarboxylase 16bc 9 chalcone reductase 31c 10 protein kinase 75b 11 auxin-related gene 61c 12 sucrose transporter HOab 13 meristem pattern gene 26 14 transcribed sequence, T45086 13 15 transcribed sequence, L36159 56 16 transcribed sequence, T45902 61b 17 StrawRipe A 10 18 StrawRipe B 40 19 StrawRipe C 48 20 StrawRipe D 54 21 StrawRipe E 62 22 StrawRipe F 81 23 StrawRipe G 90 24 StrawRipe H 92 25 StrawRipe I 99 26 StrawRipe J 106b 27 StrawRipe K 106c 1.6 Expression of ripening enhanced clones RNA was extracted from strawberry fruit during normal development and analysed by Northern blotting using standard procedures. The level of messenger RNA corresponding to the expression of O-methyl transferase, cysteine proteinase, acyl carrier protein and auxin induced gene were monitored in the receptacle at various time points between pollination and the overripe stage, between Day 1 and Day 19, and then at the stages of Turning, Orange, Ripe and Overripe Messenger RNA for O-methyl transferase appeared at Day 19, through to Overripe and was highest at Orange and Ripe. The messenger RNA for cysteine proteinase was low up to day 19, and then increased between the Turning and Overripe stages. The messenger RNA for Acyl carrier protein was low up to Day 19, and increased for Turning, Orange and Ripe. The messenger RNA for Auxin induced gene appeared around Day 16, and was highest between the Turning and Overripe Stages The data provide evidence that O-methyl transferase, cysteine proteinase, acyl carrier protein and auxin induced gene are involved in the ripening process in normal fruit development. EXAMPLE 2 Construction of antisense RNA vectors with the CaMV35S promoter A vector is constructed using the sequences corresponding to a fragment of one of the sequences 1 to 38, more especially one of the sequences 1 to 27. This fragment is synthesised by the polymerase chain reaction using synthetic primers. The ends of the fragment are made flush with T4 polymerase and it is cloned into a derivative of the pBINPLUS vector (van Engelen et ai, Transgenic Research 4, 288-290 (1995)) containing the cauliflower mosaic virus (CaMV) 35S promoter-nopaline synthase (nos) 3' terminator cassette inserted into the Hindlll/EcoRI site. For example, in this way, the plasmid pB NCEL is obtained which is derived from pBINPLUS and which contains cellulase cDNA in either the sense or antisense orientation A diagrammatic map of the plasmid pB NCEL is given in Figure 1 In one particular experiment, an antisense extended sequence comprising the cellulase of SEQ LD:6 with the addition of a polyA tail of 17 bases was inserted to give a pBLNCEL antisense cellulase vector Alternatively a vector is constructed using a restriction fragment obtained from a strawberrv ripening-related clone. The fragment is blunt ended with T4 polymerase and is cloned into a derivative of the pBINPLUS vector. After synthesis of the vector, the structure and orientation of the sequences are confirmed by DNA sequence analysis. EXAMPLE 3 Construction of antisense RNA vectors with a fruit enhanced promoter. The fragment of the ripening-related cDNA that was described in Example 2 is also cloned into the vector pJR3. pJR3 is a Bin 19 based vector, which permits the expression of the antisense RNA under the control of the tomato polygalacturonase (PG) promoter. This vector includes approximately 5 kb of promoter sequence and 1.8 kb of 3' sequence from the PG promoter separated by a multiple cloning site After synthesis, vectors with the correct orientation ofthe ripening- related sequences are identified by DNA sequence analysis. Alternative fruit enhanced promoters (E8, 2A11 or any strawberry promoter) are substituted for the polygalactonurase promoter in pJR3 or for the CaMV 35S promoter in the modified pBINPLUS vector described in Example 2 to give alternative patterns of expression. EXAMPLE 4 Construction of truncated sense RNA vectors with the CaMV 35S promoter 79 The fragment of the ripening-related cDNA that was described in Example 2 is also cloned into the vectors described in Example 2 in the sense orientation After synthesis, the vectors with the sense orientation of the phytoene synthase sequence are identified by DNA sequence analysis. EXAMPLE S Construction of truncated sense RNA vectors with fruit-enhanced promoter. The fragment of the ripening-related cDNA that was described in Example 3 is, also cloned into the vectors described in Example 3 in the sense orientation. After synthesis, the vectors with the sense orientation of the ripening-related sequence are identified by DNA sequence analysis. EXAMPLE 6 Construction of an over-expression vector using the CaMV35S promoter The complete sequence of a ripening-related cDNA containing a full open-reading frame is inserted into the vectors described in Example 2. EXAMPLE 7 Construction of an over-expression vector using a fruit-enhanced promoter The complete sequence of a ripening-related cDNA containing a full open-reading frame is inserted into the vectors described in Example 3 (pJR3 or alternatives with different promoters) EXAMPLE 8 Generation of transformed plants Vectors are transferred to Agrobacterium tumefaciens EHA105 (a kanamycin sensitive strain of an organism widely available to plant biotechnologists; Hood et al., Transgenic Research 2, 208-218 (1990)) and are used to transform strawberry plants. Strawberry explants infected with Agrobacterium are grown on regeneration medium normally containing 100 mg/l kanamycin After three weeks, the explants are transferred to regeneration medium without kanamycin. At 4 to 6 weeks, putatively transformed shoots are cultured on propagation medium for two weeks and then transformants are selected on medium containing 25 mg/l kanamycin. Regenerated plants containing the transgene are selected and grown to maturity. Ripening fruit are analyzed for modifications to their ripening characteristics For example, transformed plants were produced in this way using the pBINCEL antisense cellulase fragment of Example 2 The presence of the transgene in the putative strawberry transformant was verified by PCR using genomic DNA from the transformant as template and primers from the 35S promoter and from the cellulase strand The PCR products were separated by agarose gel electrophoresis and a fragment of -1400 base pairs was obtained that was identical in size to the PCR product obtained using the pBINCEL antisense cellulase vector DNA as template The following sequences have been edited to remove vector bases and polyA regions, as appropriate. SEQUENCE LISTING ( 1 ) GENERAL INFORMATION 0) APPLICANT (A) NAME Horticulture Researcl (B) STREET - (C) CITY Stratford-upon-Avon (D) STATE OR PROVINCE Warwick (E) COUNTRY United Kingdom (F) POSTAL CODE CV35 9EF (ii) TITLE OF INVENTION Fruit Ripening-Related Genes (iii) NUMBER OF SEQUENCES 38 (iv) COMPUTER-READABLE FORM (A) MEDIUM TYPE 1 44 MB Diskette (B) COMPUTER DELL Pentium (C) OPERATING SYSTEM Windows (D) SOFTWARE Word (2) INFORMATION FOR SEQ LD NO 1 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 549 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION O-methyltransferase (xi) SEQUENCEDESCRIPTION SEQLD 1 CCNCC CTC AATNTNNNNC ATCAT T TN NGGGGGTTGG GGNTCTNGAA 050 GGCAAAAGAT TCGGTCAGGA CAAGGTCCTC GTCGAGAGCT GGTATCATTT 100 GANGGATGCA GTTCTTGATG GTGGGATTCC ATTTAACAAG GNCTATGGCA 150 TGACTGCATT TGATTACCAT GGNAACTGAC CCTAGCATTC AACAAGGTCT 200 TCAACAAGGG AATGGCTGAC CACTCCACCA TTACCATGCA NGTAAAATCC 250 TTGTAGTACT TACAAAGGCT TCGAGGGCCT CAAATCCATC GTTGTATGTC 300 GGTGGCGGNA CCNGAGCTGT GGNGGAACAT NATCGCTTCC CNAGTTNCCC 350 TTCGCATCAA GGGTCATCAN CCTTTCGACT TGCCCTCAAT CTTANTCGAA 400 NGCATTCCTC CNTCAATTAT CCTNNNTGTT TCCANCCANG TTGGGATGNG 450 GGGANAATCT TCTGGCNANN TCTTACCCAA TTNNGGNANN CTTCCATTCT 500 TTCCCATTTN AGTTCNTNTT TTNCTCAACC TAACTTGNCG NTCCNTCGN 549 (2) INFORMATIONFORSEQLDNO2 (i) SEQUENCECHARACTERISTICS (A) LENGTH 661 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHER INFORMATION Acyl carrier protein (xi) SEQUENCE DESCRIPTION SEQ LD 2 GGTTTTAGAA CTATCCTCGA TCGCATCAAT GGCCGCCACC ACAGGAGCTG 050 CTTCTTCGAT CTCACTCCGC TCTCGCCTTC ACCAGAATCT TGCATCGTCC 100 AGGGTCAATG GTCTTAAGCC AGTTTTACTG TCTGGTAATG GAAGAAGTTC 150 TCTTTCTTTC GGGTTACAGA AGCGTTCAGC ACGGCTTCAG ATTTACTGCG 200 CAGCCAAACC AGAGACAATG GACAAGGTGT GCCAGATAGT TAGAAAGCAA 250 CTTGCATTAC CAGATGACTC GGCAGTTTCT GGAGAGTCAA AATTTTCTGC 300 ACTTGGAGCT GATTCTCTTG ATACGGTTGA GATCGTGATG GGACTTGAGG 350 AGGAATTTGG TTTTAGCGTG GAAGAGGAGA GTGCTCAGAG CATTGCAACC 400 GTTCAGGATG CTGCGGATCT TATCGAGAAG CTCATTGAGA AGAACAATGC 450 TTAGAAGAAG AAATGAGAAA ACAAGAGTCA ATCCTAGCCT GCTTTAGATA 500 ATTATTTGGT TGGTAGACTG GTTATGTATG CAGTCATTTT GTGTGAAATT 550 TGAACCTGAT AGTGGCTTGA GTGTTAAATT ATGAATGTAT GGATTTGAGT 600 TTGTGTGGTC AAGCTCCTTT CTTTCCTATA TTTCTGATGA AATAGAGAAT 650 GGCCTTACAA T 661 (2) INFORMATIONFORSEQLDNO3: (i) SEQUENCECHARACTERISTICS: (A) LENGTH: 1026 (B) TYPE: cDNA (C) STRANDEDNESS: Linear (D) TOPOLOGY: Single (ix) FEATURES (D) OTHER INFORMATION: Elongation factor (xi) SEQUENCE DESCRIPTION: SEQ LD:3: GGGCCCATGT TGACAAAGCT CAATGTCACT ATGAAGAGTG ATGAAAAAGA 050 ACTTATGGGA AAGGCATTGA TGAAGAGGGT CATGCAGAAC TGGCTTCCAG 100 CCAGCACTGC CCTATTGGAA ATGATGATCT TTCACCTTCC CTCTCCACAC 150 ACAGCTCAAA AGTACCGTGT TGAGAATTTG TACGAGGGTC CCCTGGATGA 200 CCAATATGCT AATGCTATCA GAAACTGTGA TCCAGATGGT CCGCTTATGC 250 TTGTATTGTA TCTAAGATGA TTCCGGCATC TTGACAAGGG TNAGATTCTT 300 TGGTTTTGGG TCGTGTTGTT TGGCTGGTAG GGGTCCCAAA CTGGTTTGGA 350 NGGGTTAAGG AATTATGGGG ACCCAAACTA TTGTTCCTGG GGAAAAGAGG 400 GATCTTTATG TCAAGAATTG TACAGNGGGA CTTGNNATCT TGGATGGGGA 450 AAAGAAACAA NGAAACTGTT GAGGATGTTC CCCTGTGGTA AAAACTTGTN 500 CCCTTGGTTG GTCTGGGAAN AAGTTCAATC CACCCAAGAA TGCTACCTTG 550 ACCAAATGAG AGGGNAACAA GATGCTCCCC CCATTCGTGC AATGAAGTTC 600 TCCTGTCTCA ACCCTGTTGT GCGTGTTGCT GTTCAANCGT AAGGNTGCTT 650 CTTGATCCTT CCCCAAGCTT GTTGAAGGGC TGAAACGTCT GGCTAAGACC 700 CGATCCCTAT GGGTGTCTGT ACCATTGAGG AGTCTGGAGA GCACATCATT 750 GCTGGAGCTG GTGAACTTCA CCTTGAGATC TNCNTGANGG ATCTNCAAGA 800 TGATTTTATG GGTGGAGCGG AAATTGTAAA ATCTGATCCT GTTGTGTCCT 850 NCCGTGAGAC AGTCCTTGAG AAGNCCTNCC GTACTGTGAT GAGCAAGTCT 900 CCCAACAAGC ACAACCGTCT GTACATGGAA GCACNCCCGT TGGAGGAAGG 950 TCTTCCTGAG NCCATTGATG ATGGTCGTAT TGGNCCAAGG GATGATCCTA 1000 AAATCCGCTC AAAGATCTTG NCTGAG 1026 (2) INFORMATIONFORSEQLDNO4 (i) SEQUENCECHARACTERISTICS. (A) LENGTH 957 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION: Auxin-induced mRNA (xi) SEQUENCE DESCRIPTION: SEQ LD:4: GGCACCAGTG CTTCATATCT CGCCCTTTGC AGTTTCACAT ATCAAAGTAG 050 CATCTCAAAT CACATCAATG GCAGACGAGG TTGTCTTGTT GGACTTCTGG 100 CCAAGCCCAT TTGGGATGAG GCTGAGGATC GCTCTGGCCG AGAAAGGCGT 150 CAAGTACGAG TACAAGGACG AGGACCTGAG GAACAAGAGC CCGCTGTTGC 200 TTCAGTCGAA CCCGGTTCAC AAGAAGGATC CCGGTTCTCA TTCACAACGG 250 CAAACTGTCT TGCGAGTCTT GTCATTGCTC TTCAAGTACA TTGACGAGGT 300 CTTGGACTTA ACAAAGCCAC TATTGNCCTC CCGACCCCTT ACCTCAGGAT 350 CCCCAGGCCA GGGTCTTGGG CCGACTTCCG NGGACAAAGA AGATNTTTTG 400 ATNTCGGGTA GGNAAGACAA TGGNCAACGA AAGGAGATTG AGCAGGGAGG 450 CAGNAAAGAA GGGATTCTTC GACTGCATTA AGTTGCTAGA AGTGGAGCTT 500 GGTGACAAGC CTTTCTTTGG CGGTGAGACC CTCGGATTTG TGGACGTGAC 550 GCTCGNTCCT TTCTATTCCT GGTTCTCTGT GTATGAGAAA TACGGCAACT 600 TCAGCATTGC GCCAGAGTGC CCAAAGTNCA TGGCTTGGGT TAAGAGGTGT 650 ATGGAGAAGG AGAGTGTGTC AAAGTCTCTT CCTGACCAGG ACAAGGTCTG 700 TGGCTTNGTT GCCGAGATGA NGAAGAAGCT TGGAGTTGAG TAGATGTGAT 750 CAATGTCATN TTGATCATGT CITTGTTTTA GCCCCAAGAT TCANCCTCGT 800 TTTGGGTTGC TTGTATTTTT CAATAAAATT GGGGGACTTG GACCAAGCCC 850 TCCAATAGTA GGAAGCACTC TTTCNGTGCC TCTTGGTCCN GTTTTTCTTC 900 NGNTAANCCT NTNTGCAGCT AAAATTCACC GNATTNCTGN TTTCCTTNTA 950 TNGCCAA 957 (2) INFORMATIONFOR SEQ LDNO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH 518 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Cysteine(thiol) proteinase (xi) SEQUENCEDESCRIPTION SEQID 5 ATCTCCTCCT CCTCTCTCTC CTTCTCCTCC TCTCCTCCGC CGTCGCCTCC 050 ACCGTAACCG ACGCCGGCGA TCCTCTCATA CGACAAGTCG TACCGGGCGC 100 GGCCGAGGAT GACGAGCTCC TCCACGCGGA GCGTCACTTC TCGAACTTCA 150 AAGCCACGTT CGGAAAGAGC TACGCGAGCC AGGAGGAGCA CGACTACAGG 200 TTCCGGCGTA TTCAAGGNCA ACTCCGCCGG GCGAAGAGGC ACCAGGGGCT 250 TGGACCCCAC CGCCGTGCAC GGTGTCAACG AAATCTCCGA TCTCACTCCC 300 AAGGAGTTTC GNCGGGAATT TCCTCGGGCT TAAGAAGGGG TCGGANTTCG 350 GGTTACCGGC CGACGGTTAA AAAAGGGGCC NGATNCCTNC CGGANGAATT 400 ANCTTCCCCA CCC.ANTTTTG GNNTTGGGGN GAAAAAAGGN GCCCGNCNAA 450 GNCGGNGGAA NGGNCAAGGG GGAAATNGGG TNNAATTNGG NCNGGTTNAN 500 NGNGGGCCCG NAGAANTT 518 (2) INFORMATIONFORSEQIDNO 6 (0 SEQUENCE CHARACTERISTICS (A) LENGTH 1766 (B) TYPE cDNA (C) STRANDEDNESS Smgle (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION: Cellulase(endo-(l,4)beta-n-glucanase (xi) SEQUENCEDESCRIPTION: SEQLD:6: GGCAGCAAAA ACGAGAGAGA AAAAAAAATG GCGCGAAATG GCCTTTGCTT 050 ACCGGGAAAT GCTCCCGCAT TTCGCGCAAC ACTCGTCCTC TCGCTGCTCC 100 TGCTTCTCCA GCCAATCCGC GCCGGCCACG ACTACCACGA CGCCCTCCGC 150 AAGAGCATCC TCTTCTTCGA AGGCCAGCGC TCCGGCAAGC TCCCGCCCGA 200 TCAACGCCTC AAATGGCGCC GCGACTCCGC ATTGCACGAC GGCTCCACCG 250 CCGGCGTAGA CTTAACCGGC GGCTACTACG ACGCCGGCGA CAACGTGAAG 300 TTCGGGTTTC CGATGGCGTT CACGACCACT CTGCTGGCGT GGAGCATTAT 350 AGACTTCGGG AGGGTCATGG GGACGGAGCA GAGGAACGCG GTCAAGGCGT 400 TACGGTGGGG GACAGACTAC CTCCTGAAGG CCACGGCGGT TCCTGGCGTC 450 GTCTTCGTCC AAGTCGGCGA CCCATACTCC GATCACAACT GCTGGGAGAA 500 GCCGGAAGAC ATGGACACAC GCCGCACGGT GTACAAAATC GACCACAACA 550 ACCCGGGATC CGACGTGGCA GGCGAAACCG CAGCCGCGCT CGCCGCCGCC 600 TCCATCGTTT TCAGGTCACG TGACCCCGCT TACTCGAGAC TGCTTCTCAA 650 TCGAGCCGTT AAGGTTTTCG AGTTCGCTGA TACCCACCGC GGCGCGTACA 700 GCTCCAGCCT CAAAAACGCC GTGTGCCCTT TTTACTGCGA CGTCAACGGC 750 TTCCAGGATG AGTTACTGTG GGGAGCAGCG TGGTTGCACA AGGCGTCGAG 800 AAGGCGGCAG TACAGAGAAT ACATAGTGAG AAACGAGGTC ATTTTGAGAG 850 CTGGAGATAC CATTAACGAG TTTGGTTGGG ATAACAAGCA TGCTGGGATT 900 AATATTCTCA TTTCTAAGGA AGTGCTTATG GGAAAAGCAG ATTATTTCGA 950 ATCTTTCAAG CAAAATGCAG ATGGATTTAT ATGCTCTGTT TTGCCTGGAC 1000 TTGCCCATAC CCAAGTCCAA TATTCTCCAG GTGGTTTGAT CTTCAAGCCT 1050 GGAGGGAGTA ACATGCAGCA TGTAACTTCG CTATCGTTCC TGCTTTTGAC 1100 TTATTCCAAC TATCTAAGCC ACGCCAATAA GAACGTGCCG TGTGGCATGA 1150 CCTCCGCCTC CCCGGCCTTC CTCAAACAAT TGGCTAAACG CCAGGTGGAT 1200 TACATTTTGG GTGACAATCC ATTAAGAATG TCTTACATGG TTGGATATGG 1250 GCCGCGTTAC CCGCAAANGA TTCACCACCG GGGCAGCTCA CTTCCATCCG 1300 TGCAGGCCCA TCCGGCCCGT ATCGGATGCA AAGCCGGTTC TCATTATTTT 1350 CTGAGTCCGA ATCCAAACCC GAATAAATTA GTCGGGGCTG TTGTGGGCGG 1400 ACCCAATAGC TCGGATGCAT TTCCGGACTC GAGGCCTTAC TTTCAAGAGT 1450 CTGAGCCCAC GACGTACATA AATGCGCCTC TTGTGGGCCT ACTTTCGTAT 1500 TTTGCAGCCC ATTACTAATT CTCGAAGTGT AAACAGTGAT TGAGAATTTG 1550 TTGTGGTGCG CCAATACTCA CCCACCAATC CCCCACACTA CCAATTGTTG 1600 TTACTTTTGG AAAGTTCTAA ATTTAAGAAA TTGTTAAGAA AGAAAATGGC 1650 CCAAGCTTAG TTATGGAATT TAGTCTCAAA AGCCCTACTG TTGTGCTTTT 1700 GAAATGTTCT AGCTGTAACA TAATTTCTAT CAATGAATAA AGAAAATGGG 1750 CCAAGCCTAA ATGTGG 1766 (2) INFORMATIONFORSEQIDNO7 (i) SEQUENCECHARACTERISTICS (A) LENGTH 585 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Starch phosphorylase (xi) SEQUENCEDESCRIPTION SEQLD 7 AATCCTGGGG GGNTTNCCCA CCCTTAANTT GGCNGNNGAT NTTTTTGATA 50 CTCNTCGGGG GGGCGGAANC CTATGGGGAG AANNGGCAAC CAAAGGNGCC 100 TTTTNTAGGG TTGCCTGGCN TATTTACTGG CCTGGTNCTN AACATGTNCT 150 TTCCTGCGAT ATCCCCTGAT TCTGNGGATA ANCCGTATNA CNCGCCNNTG 200 AGTGAGGCTG ATACCGCTNC ACCGCATCCG ACCGACCGAT CGCAGCGAGT 250 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAGCC ACCTCTCNCC 300 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG 350 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT 400 AGNCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA 450 ATTGTGAGCG GATAACAATT TCACACAGGA AACANCTATG ACCATGATNA 500 CNCCAAGCTA TTTAGCTGAC ACTANAGCAT ACTCAAGCTT GNATGCCTAC 550 AGNTCGACTC TAGAGGATCC ACCGGGTACC GAGCT 585 (2) INFORMATIONFORSEQLDNO8 (i) SEQUENCECHARACTERISTICS (A) LENGTH 693 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHER INFORMATION Pyruvate decarboxylase (xi) SEQUENCEDESCRIPTION SEQLD8 CATCTTTTCA CTCGAAGTCT CAATCTTTCA TCACAAACAT TCCCATTTGA 050 TCACAAAAAA GTTTCAACCT TTAAACCTCC ATGGACACCA AGATTGGCTC 100 CATCGACGTC TGCAAAACCG AGAACCACGA CGTCGGTTGT TTACCAAACA 150 GCGCCACCTC CACCGTTCAA AACTCAGTCC CTTCGACCTC CCTCAGCTCC 200 GCCGACGCCA CCCTCGGCCG CCACCTGGCA CGCCGCCTCG TTCAAATCGG 250 CGTCACCGAC GTCTTCACCG TCCCCGGCGA CTTCAACTTG ACCCTTCTCG 300 ACCACCTCAT CGCCGAGCCC GGCCTCACCA ACATTGGCTG CTGCAACGAG 350 CTCAACGCCG GGTACGCCGC CGACGGCTAC GCGCGGTCGC GTGGCGTCGG 400 CGCCGTTGCG TGGTGACTTT CACTGTTGGT GGACTGAGTG TGCTGAACGC 450 GATCGCCGGC GCGTTATAGT GAGAATTTGC CGGTGATTTG TATTGTTGGT 500 GGGCCCCAAC TTCTAATGAT TATGGGACTA ACCGGATTCT TCACCATACT 550 ATTGGGTTGC CGGACTTCAN TTCAAGAACT CCGGTGGTTT CAAGAACNTG 600 ACTTGCTTTT CAGGCTGTGG GTGAATAATT CTTGGAAGAA TGCACATGAA 650 TTTGCTTGAA TACNGCAATT TTCAATNGCN TTNGAAANAA AAC 693 (2) INFORMATIONFORSEQLDNO:9: (i) SEQUENCECHARACTERISTICS: (A) LENGTH: 693 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (ix) FEATURES (D) OTHERINFORMATION: Chalconereductase (xi) SEQUENCEDESCRIPTION: SEQLD:9: CCCAAATCCC AGAAGTGGTT CTTGAATCCT CCAACGGCCG CAGAACCATG 050 CCTGTGCTTG GATTCGGCAC AGCATCCAAC AATTTACAAC CGGAGGTTTT 100 GATAGAAGCT GTTCTTGAGG CCATCAAGCT TGGTTACCGA CACTTCGACA 150 CTGCTTCCAT TTACGGCTCC GAGCAGACTC TAGGAGTAGC CATTGCCCAA 200 GCGCTCAAAC TCGGCCTCGT GGCTTCTCGT GACGAGCTCT TCATCACTTC 250 CAAGCTTTGG CCTAATGATG GTCACCCCAA CCTGGTTATT CCTGCTCTCA 300 AGAAAATCGC TTCAGAATCT TGAGTTGGAG TACCTTGATT TGTATCTGAT 350 ACACTGGCCC ATCAGTGCCA AGCCTGGGAA AGTTGAGTCA CGCACTAGAG 400 GGAGAAGGAC CAAATGCCGA TGGACTTCAA GGGTGTGTGG GCAGACATGG 450 AGGAAGCTCA GAGACTTGGC CTCACCAAAT CCATTGGGAA TCAGCAATTT 500 CTCTACCAAA AAGACTCAGA ATTTGCTCTC CTTTGGCTAC TATTCCTCCG 550 TCAGTCAATC AANTTTAANA TGANTCCATT TTGGCAACAG AAGAACCTCA 600 AAAACTTCTG CAAGGCCAGT GGTATAATTT GTGACTGGCT TCTCCCCATT 650 GGGTGCCATN NGAACCANTT GGGGGCACCA ATCATGTTCT CNA 693 (2) INFORMATION FOR SEQ LD NO 10 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 763 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Proteinkinase (xi) SEQUENCEDESCRIPTION SEQLD10 GCANANCGTG TTGTGGGAAC TGGGTCATTT GGAATTGTAT TCCANGCGAA 050 ATGCTTGGAA ACTGGTGAGA CTGTGGCCAT AAAGAAGGTT TTACAGGACA 100 GAAGGTATAA GAACAGGGAA CTTC-AATTGA TGCGCGTAAT GGATCATCCA 150 AATGTGATTT GTTTGAAGCA TTGTTTCTTC TCTACAACAA GCAAAAATGA 200 GCTTTTTCTC AATTTGGTTA TGGAATATGT TCCGGAAACT ATGTATCGGG 250 TTATAAAGCA TTACAGCAAT GCAAACCAGA AAATGCCCCT TGTCTATGTC 300 AAACTTTACA TGTNCCACAT TTTCAGAGGG CTGGCTTACA TACACACCGT 350 TCCTGGAGTT TGCCATANAN ATTTGAANCC TCCAAATTTA TTGGTTGATC 400 CTCTTATTCA CCANGTCAAG CTTTGTTGAT TTTGGAAGTG CCAAAATGCN 450 GGTGAAAGGN GAAACAAACA TANCATACCT ATGTTTCACG TTTCTATCNG 500 GCTCCNCGAA ACTAATTTTT TGGTGCCNCC NGATTATACC ACTTCCCATT 550 GATATCTGGT CNGCTGGCTG TGTCCTAANC AAAACTTCCT TTTGGGCCCC 600 CCTTTGTTTC CCTGGAAAAA AATGCCATNG AACCACCTGT TAAAAATCNT 650 TCCNGGTTCN GGGGAACACC NCNCCNTTCA AAAAATCCCC NTTTTGAATC 700 CCCANTTNTA CCAAATTCCC GGTTTCCNCC GAAAAAANCC CNCCCTTTGG 750 NNNAAGGTTT TCC 763 (2) INFORMATION FOR SEQ LD NO 11 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 772 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Auxin-relatedgene (xi) SEQUENCEDESCRIPTION SEQID.11 GGTGAAACTT TACTTTTGCA ATACACCGTC TAACAATGGC TGCAGCTCCA 050 AGTGAGTCCA TACCCTCTGT AAATAAGGCC TGGGTCTATT CAGAGTATGG 100 AAAAACTTCT GATGTTCTCA AGTTTGATCC AAGTGTGGCT GTTCCTGAAA 150 TTAAAGAGGA TCAGGTGCTG ATCAAGGTTG TTGCTGCTTC TCTTAACCCA 200 GTTGATTTTA AGAGGGCTCT TGGTTACTTC AAGGACACTG ACTCTCCCCT 250 ACCTACAATT CCAGGGTATG ATGTANCTGG TGTGGTGGTA AAGGTAGGAA 300 GCCAAGTAAC CAAGTTTAAG GTGGGGGATG AAGTGTATGG GGATCTCAAT 350 GAAGACAGCA TTGGTGAACC CAACAAGGTT TGGGTCTTTG GCANANTACA 400 CTGCTGCAGA TGAAAGANTA TTGGCTCACA AACCCAAAAA CCTGAGCTTT 450 ATTGAAGCTG CTANCCTTCC CTTGGCTATT GAAACTGCCC NTGAANGGCT 500 TGAAAGAACT GAACTTTCTG CTGGTAAATC CGTCCTTGTT TTGGGAAGCG 550 CTGGGGGTGT TGGAACACAN ATTATTCAGC TGCAAAGCAT GTTTTTGGTG 600 TTCCAAAGTA GCAGCTACTG CAAGCANTAA GAAACTGGAT TTGTTGAGAA 650 CNTTGGGNGC TGATTTGGCT ATCGATTACA CCAAGGAGAA NTTNGAGGAC "00 CTGCCAGAGA AATTTGATGT AGTGTATGAT GCAGTTGGGG AGACAGATAA 750 GGCTGTGAAG GCGGTGAAAG AAGGCGGGAA GGTTGTAACA ATAGTAGGTC 800 CAGCAACGCC ACCGGCTATC CTTTTTGTGC TTACCTCTAA AGGGTCTGTG 850 TTGGAGAAAC TGAAGCCTTA CTTGGAGAGT GGGAAGGTGA AGCCAGTTCT 900 TGATCCCACA AGTCCATATC CCTTTACTAA AGTTGTTGAA GCATTTGGTT 950 ACCTTGAGAG TTCCAGAGCT ACCGGAAAGG TGGTTGTGTA TCCCATCCCA 1000 TGAGGTTGAG AGTGTATGTG TGAATGATCT ATGAGACTAT GATTGTGTAG 1050 AGTCCATTTC CTTCCTCTTG TATGTGTGTA GCAGTATATT TTAATCTTGA 1100 AGCCTTGTAA TAATGAATAA GATTGAGTCC TTAATAAATT GTCATTACAT 1150 G 1151 (2) INFORMATIONFORSEQLDNO 12 (i) SEQUENCECHARACTERISTICS (A) LENGTH 1167 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Sucrosetransporter (xi) SEQUENCEDESCRIPTION SEQLD 12 CCATTGGCGA TCACGTACAG TGTTCCATAT GCCTTGATTT CTTCTCGTAT 050 CGAGTCTTTG GGACTTGGCC AAGGCTTATC AATGGGTGTA CTGAATCTGG 100 CAATCGTAGT ACCACAGGTG CTGGTATCCC TGGGAAGTGG ACCATGGGAT 150 CAGCTATTGG TGGTGGAAAC TCTCCAGGGT TTGCGGTTGC AGGAGTTGGA 200 GCCTTAGCAA GTGGGCTGGT GGCCAATCTT GGCTATTCCA CGTTCTATTC 250 CACAGAAGCC TANATCTTTC ACATGAGGTA TTTTGTTGTA TCTACTTTTT 300 ACCCAACTTT GTCACAGAAA TACAAAACCT CCATAGATAG TGAGAATTTG 350 TAAATATCTT TTGTTACGTG TTAGCTATTT CTCAATACAC TCATTTACCA 400 GAGGTTTCTT TAGTTCTGGA AATTTCTCTC TTTCCCTTTT TGTCGTTTTA 450 GATGCTTTAA TAAAGAAAGG CCTGGCAGCG ATTATATCAA AGTTGANCTG 500 AATATCTGTG TTGAAGTGCT TCCGTTCAAC AATTTATAGT TCTCAATTTC 550 TACAATATTT TAAATCAGAA CTGTCACCTG GTGGACTCTT ATGGAATCCA 600 TATGTTGGAA CCATAATCTC AATTAGGCAT CGTGCCTCAA TTCCACAATG 650 GTGTTTTCAG AAGTGTGATG AAACAAGTTA GTCAAGAAAG TGATGGTGTT 700 TTCACAAATG CTGGCTACGC AACGATATTG ATGTGGGTAC GCAAATTGAT 750 TGATGTAGTA GCCATCACTA AGTTCCTGGT TAGACAAGTT ATCTACAATT 800 AGTGGANAAT TTCTTGAATG AAAATCAGTC CCATCTGGTG GATTGTGGCA 850 AATTGCTACG GAAAAGTAGG TGAAGCCTCA GCTGTAGGAT TTGGAAATTA 900 CTTGAAGAGT AGTTCCCTAC CAACCAGGAT ATGTTTCTGC TTTTCGAGAA 950 TTTGTCCTCC TGAAAATATC GTTTTTTCTT TTGGCAAAGT TGATTTTGAC 1000 TTAGTGGTTT AATCATGAGG TATTGGAATC TCATGCGTTT TGTGCATGTA 1050 TTTGTANTAT GAATGTGGTG AAATGTGCTT GGTGGCCAAC AGTGAATATA 1100 TGAAATGTAC TGATTGAAAC CTTGATGGAN ACATCCCTTT TAATTGCTGT 1150 TTTGGAAGCT TGGGTCC 1167 (2) INFORMATIONFORSEQLDNO:13 (i) SEQUENCECHARACTERISTICS. (A) LENGTH: 476 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (ix) FEATURES (D) OTHER INFORMATION meristem pattern gene (xi) SEQUENCEDESCRIPTION: SEQID.13 CCTCANNAAT CTCTATATTT TTTGGGGGCG TGGGTGGTCT AANAATATGT 050 TCTTGGCTTC AAAACCCTCA TCAGATGGAG AGCACCGACT CGTCTTCCGG 100 CTCGCAGGCG CCGCCGCAGC CAAACCTACC TCCGGGATTC CGCTTCCACC 150 CCACCGATGA GGAGCTAGTC GTTCATTACC TCAAGAAAAA GGCCTCCTCG 200 GCTCCCCTCC CCATTGTCAT CATCGNCGAA GTCGACCTCT ACAAATTTGA 250 TCCATGGNAG CTCCCAGAAA AGGCGACGTT CGGAGAGCAA GAGTGGTACT 300 TTTTCAGTCC TAGAGACCGG AAAGTACCCN AACGGAGCAC GGNCTAATAG 350 AGNAGGGACT TCAGGNTTTT GGTAGGGGAA CCGTANTGAA AAGCCCTTTT 400 GGGTTGNACT ATTANGAGGN NGGGGGNTCT CCCAAANTTG NGGTNAAAAN 450 GNANTTNTTT NTTTNANGGG ACNNCC 476 (2) INFORMATIONFORSEQLDNO:14 (i) SEQUENCECHARACTERISTICS. (A) LENGTH: 497 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (ix) FEATURES (D) OTHERINFORMATION transcribedsequence,T45086 (xi) SEQUENCEDESCRIPTION: SEQLD14 TNAATTAANG GCAGCCNATT CGGTGAATTT CCTTCATTCG ATCCTGCAAA 050 CATGCCTTAT GGNAACGCTT GAAGTCCTTC TGGTTGGGGN CAAAGACCTT 100 GNAGACCATG ATTTTTTCGG TAAAATGGAT CCCTATGTCC TTTTATCATT 150 AAGGACCCAA GAGAAGAAGA GCACTGTGGC ATCAGGACAA GGATCTGCAC 200 CAGNANTGGN AATGAAACTT TTCAATTCAC AGTCTCATCA GATGATGTTA 250 CCGAACTCAG CTTAAAAATC TATGACAAAG ATACCTTCAC CCCAGATGAA 300 TTTCTTGGAG GAAGCAACCA TTCCTTTAGN AAACAGTGTT CATGGGAAGG 350 AAGCACTGAA CCGACTAAAT ACAATGTCGT CAATGAGAAT AATGAATATC 400 ATGGAGGATA TTACAGTTGG ACTCACTTTC ACCCGTGAAG CGAACCGGCT 450 CTCGTGCGGG NGGNTNTGAT GAAGAAAGAA CAA 483 (2) INFORMATIONFORSEQLDNO:15: (i) SEQUENCECHARACTERISTICS: (A) LENGTH: 488 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (ix) FEATURES (D) OTHER INFORMATION: transcribed sequence L36159 (xi) SEQUENCEDESCRIPTION: SEQLD:15: AGGATATGTT GATTAGAACT CATGTAACCT CATATTACAC ATCTTAATAT 050 CTCCAATTAC ATGAACGTAA AATAAAACCC CTAACTTCCA CAAAGCATCA 100 ATCANACACG GGGNACGTCC GCGAATGCTA AGCAACTTGA CATCATCGAT 150 CACCGGACCA CACAGAGAGC CGGAGTGATC GCTCGTCATG GTGTACATTG 200 TGCTCAGAAA CATGACACGC GTGCGCGGCG NACACGGNGG TGNAAGAAGA 250 GCCTGGCCTT CTTGNAACCC TCCTTTGCCT TTGGACTCAT AAGGAACCTT 300 CACAGTCTCC TTGCCGGCAA ATGCCTCGAT AAAGAGGGAG CCTTCGCAGT 350 CGTTGGTTCC CGTCGNCGAC AGAGAATNTN AGGGCGTAGC GCCTNNCGGG 400 NTTGGTGAAG ACCACTTGAG CCAATGNGCT CTCTTTTCCC GGCAACGAGC 450 TCGNTNGGTN TTAGGCCTCC NGGANGGGAA GTGTGGNG 488 (2) INFORMATION FOR SEQ LD NO 16 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 460 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION transcribedsequence, T45902 (xi) SEQUENCEDESCRIPTION SEQLD16: GTTTGTCCTC GGTTCCTAAA GAGAGAGACA CCCAGAATTT GNTTCAGAAA 050 TCGGAGATTA AGTTCCTGAA CCAAGTTCAA GGCCCTGAGA GCGTCGCCTT 100 TGATCCACAA GGACGTGGAC CATATNCTGG TGTTGCGGAT GGGAGGAGTC 150 TTGTTCTGGA ATGGGCAGGC CTGGACTGAT TTTGGCTATN CATCGCCNCA 200 CAGGTCAAGA TATATGTGNA TCCCANAACC ATCAGCTATG ANTTACTTGG 250 CAAATGAGCA CATCTNTNGN AGGGGCCNTG GGGCTCCCCC TTTTGGNAAA 300 GAAAACAGGA GATTTGGNGC AATTTGGGGG TTGAATACTT TTGGGCTTTN 350 TTNGAAAATG GGGCAAANGN TNNGGTTTNG GGAAATTTCC ACTTCNAACT 400 TAGGANAANG GGGNGCCATG NGGGTTTCTT ACCCTCTTGG NNTGGTGAGG 450 ANGGANAATT 460 (2) INFORMATIONFOR SEQ IDNO.17 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 480 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQLD17 NTGGGTTCCA TGACACTTCC TAAAGAGCTT CCCACCATCA ATTTCTCCCT 050 CCAAGACTTG AAGCCTGGCT CAAGCTCCTG GACTTCCACC TGCAAACAAG 100 TCCGCAATGC ACTCGAAGAA TATGGTTGCT TTGTGGCATT GTNCCCACAA 150 GTCTCCCAAG AGCTCATGGA CAGTATCTTC GGNCAATCCA GGGATCTGTT 200 CGAGGTTCCC CTCGAGAACA AGGTCAAGAA CACCAGCGAG GAGCCTTACC 250 GTGGNTATAT CGGACCAAAC CCCCTCTTGC CACTCTATGA AGGCATTGGC 300 ATTGACAACG TCACATCCCA ACAAGAAACT CAGAAAGTTC AGGGACCTCA 350 TGTGGGCTAA TNGAAAGACC CAATTCTGTG AAAATCACAG ATCTTGTTNG 400 GCANGTNGCT CGGGGAGTTN GGAAAACACT GTGGAAANGA TGNTNTTNCG 450 NAAGTTACGG GNTACCTCTT GGGGANNTNA 480 (2) INFORMATIONFORSEQLDNO 18 (l) SEQUENCECHARACTERISTICS (A) LENGTH 673 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQLD18 GATTCGGGTA CANTTACAGT ACCAGATATC AATATCAATA CTAGATAACA 050 GTATATNGCA CGTCTTCTTC TTCTTCTTCT TCTTCTTCTT (ZTTTTTTGGT 100 GGAAGCTCGT CITCTTCTTC TTCTAGCTAG CTTTCTTCAG CTTTTTTTAT 150 TTGTTATTCT TCATCTTCTA CCCTAATATA CTCTTTGATA CATAAAAGTC 200 CAGCACTTTT CAAACAATAG CAACTCAGTA GTCTTTACCC TCAGTAGTGA 250 TTAAAAACTA CTGCGTCGTC ACTCCACAAG AGCTTGTATT ACCACNTAGA 300 TGGCCTCATT GCGCTCTCTC GCATTCCAGG TGAATCACTT CGAGCTGCAA 350 CTTATAACGC CGGCAAAGNC AACACCGCTC GAAATGAAGC TGTTGGTCGA 400 ATATCGACGG ACCAGCAATG CCTCAGGTCT CATGTTCCCC ATTCATCATG 450 TCTTACAAGA ACAATCAATC AATACTGTCG GAAACCAAAC GACCCGNNGG 500 AGGTGGATTA GGGGATGCGC TGAGCAAGGG ACTGCAGTTT TACTACCCCT 550 TGGGTGGTNG GTTCANGGNG GGGCCTAACA AAAGGNTATG GNGGACTGAA 600 CCGNGAAGGA ACTTGGTCGN TGGGGGAACG CCGAGGCAAA NCGAGGACTC 650 GGGNTGAACC CANCGCCNGG CCA 673 (2) INFORMATIONFORSEQLDNOT9 (i) SEQUENCECHARACTERISTICS (A) LENGTH 749 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQ D:19 AAATTGAGGT CAGTATAAAT TCCAAACACA CCATCAAACC ATCAACTTCC 050 TCTACACCAC TTCAGCCTTA CAAGCTTACC CTCCTGGACC AGCTCACTCC 100 TCCGGCGTAT GTCCCCATCG TATTCTTCTA CCCCATTACT GACCATGTCT 150 TCAATCTTCC TCAAACCCTA GCTGACTTAA GACAAGCCCT TTCGGAGACT 200 CTCACTTTGT ACTATCCACT CTCTGGAAGG GTCAAAAACA ACCTATACAT 250 CGATGATTTT GAAGAAGGTG TCCCATACCT TGAGGCTCGA GTGAATTGTG 300 ACATGACTGA TTTTCTAAGG CTTCGGAAAA TCGAGTGCCT TAATGAGTTT 350 GTTCCAATAA AACCATTTAG TATGGAAGCA ATATCTGATG AGCGTTACCC 400 CTTGCTTGGA GTTCAAGTCA ACGTTTTCGA TTCTGGAATA GCAATCGGTG 450 TCTCCCGTCT CTCACAAGCT CCATCGATGG AGGAACGGCA GAATGTTTTC 500 TCAAGTCCTG GGGTGCTGTT TTTCCGAAGG TTGTCCGTGA AAATATCATA 550 CATCCCTAAT CTCTCTTGAA AGCCAGCATT GCTTTTCCCC ACCGAAAANA 600 TGACTTGCCT GAAAAGTTAT GCCGATCAGA TGGAAGGGTT ATGGTTTGCC 650 CGGAAAAAAA TTGCTACAAG GAAATTTGTA TTTGGTGTNA AAACCATATC 700 TCCATTCCAG AAGAAACGAA AACGANTCCG TGCCCAAGCC ATCACAATT 749 (2) INFORMATIONFORSEQLDNO20 (i) SEQUENCECHARACTERISTICS (A) LENGTH 218 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQLD20 TCGGGCAGAG AAATCTTTGA GATTGGCAGA CTCGAGAGCA TCCAGACTTC 050 GAGAAAGAGT AGAGGAGCTT ACCTGTCAAC TGGAAGAATT TGAAAATCGG 100 GAGGACTTAA GGAGAGGCCT GGGTGGACCT AGATATGTAT GTTGGCCCTG 150 GCAGTGGCTT GGGCTGGACT TTGTAGGGTT CAGTCGCTCT GATACAGAAC 200 AACAGAATAG TTCAAACG 218 (2) INFORMATIONFORSEQLDNO21 (I) SEQUENCECHARACTERISTICS (A) LENGTH 437 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY: Linear (xi) SEQUENCE DESCRIPTION: SEQ LD:21 : CGCTGCTTGT CTCGCTGCCT ACTATCACTA CTACCATGGG TTGGTCCCCT 050 TTCCTTCAGA ATCGGACATG TTTTGGGACG TTCAGATTCC ATCTATGCCG 100 CTGTTGAAGT ACGATGAGGT ACCCAGCTTC TTGTACCCTA CTAGTCCTTA 150 CCCGTTTTTG AGGAGGGCCA TTTTGGGACA ATACGGGAAC TTGGAGAAGC 200 CCTTCTGTAT ATTGATGGAC ACTTTCCAAG AACTCGAGAG CGAGATCATC 250 GAGTACATGG TTCGTTTGGT GCCCCATCAA NGTTGTTGGT TCCCCCTTCT 300 TTCAAAGAAC CCCAAAAGCC CAAAANCGCT NTTCCCCCGG GGGATTTCCA 350 TNAGGGCCGA CGNANTTCAN CCANCCGGTT NGTTTCGGAA ACNAAAACNN 400 AACANNTTTC GNGGNTTTTT NACACCCANG NTNNCGG 437 (2) INFORMATION FOR SEQ LD NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 232 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (xi) SEQUENCEDESCRLPTION: SEQLD:22: AAGAAAGGAG TCTCGTCAAT AAAGGATTTG TGAGAATCAA ATAACGTTCT 050 CTGTTTATTA ATTTGTAACA GTAGTTTGAT CGAGTCTGTG AGTAAGTGAT 100 CGAGTAAGAG ATGTACTCTA CTGTGTGTGT GTCAATCATG TTCGTGTTCT 150 TTGGTAGCCA TGTAATGTTC TCCATCTGGT CATTATCTGT GGCCTTGTGA 200 TCATGTTTAA TCAATGAAAC TACTATTAGT AT 232 (2) INFORMATION FOR SEQ LD NO 23 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 469 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION. SEQLD.23 GATCGGTCCG ATGACCGGAA AAGTCATGAT TTTGAGGTGA GGAGCGANTT 050 GGGTTTCGCC NGAAATGTNC AAAGCCCTGT GCTTTCGGAG CATGTGGTTG 100 AGAATTTGGN GAAAGGCAAA GTGGGTGTCC AAGAAATTGG NGAANTTGGN 150 AGCTTTGATA AGGATTTGGG ATAANTTCTN GTTTGATTCC CGCCNGAGAA 200 AGCTCGNTCT TCTTTTGAAA TTTGACAANG AGGAGGGGTT CANCNCNAGT 250 CCAACAANNG AATCAAGGGA GGANANACTC ANCTTNAGAC TCANCGTTCG 300 CNCAGANGNA GNAANNTAAA AACTGNGGCG AAAACCGNCT NNCGAGGTGA 350 TAATTAANNT CCACCTTCTT TNTTNCACGG TCCCCCCGCT TNTTTTTNNA 400 GCTTTTTCTC CNTCAANGCN AATTCCCGTT NGNTNTTCTT NTTNTGCCNA 450 NNCTAATNCN CTTNATTCC 469 (2) INFORMATIONFORSEQLDNO.24 (i) SEQUENCECHARACTERISTICS (A) LENGTH. 178 (B) TYPE. cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCE DESCRIPTION SEQ LD 24 AACCAGATAT NAAGCGATTT TCGATATTCA ATAACATTCT TCTTTAACTG 050 TTCAGGTGCG TCAGGAGCCC AACGCTCAGG GTAATCGGCG AAAGTGAATN 100 TTGGNTNGAC ATTAGNAACC AGCCAGACCA ATAGCCGTTG GAACAGCTGA 150 CGTTCGGCGC GCCCAACCGG TGGNGCAA 178 (2) INFORMATIONFOR SEQ LD NO.25 (i) SEQUENCECHARACTERISTICS (A) LENGTH 244 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQLD.25 TTCAAGTTAA CCTCTCAAAC CCGACACAGA GAGCATAAAT GGGTTCCGAA 050 TCATTGGTTC ATGTTTTCTT GGTTTCCTTC ATCGGCCAAG GCCATGTGAA 100 CCCACTCCTC CGNCTCGGNA AGCGCCTCGC TGNCAAAGGT CTCCTCGTCA 150 CCTTCTGCAC CGTCGAATGC GTCGGTAAGG AAATGCGNAA GTCCAACGGC 200 ATCACCGACG AGCCCAAACC AGTTGGAGAT GGATTCATCC GCTT 244 (2) INFORMATIONFORSEQIDNO26 (i) SEQUENCECHARACTERISTICS (A) LENGTH 685 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQLD26 CCAATTCGGT CGCCGTAAAA CATGGTTAAT CAAACGGTGA ACGGAAGCCA 050 ATCAAGTAGC GGAACCCAAA AGCTCAATGC TTCAAGCAAC ACCAAGAGGG 100 ATTTTGAGGC TGTGAGTGAG TCCATGCACT CTGCAATTTC AATGAGTAAA 150 ACAGAAGTCT TGGATTCTGT GCTGAGTGAT TTCTCTGAGG GATATTTTAG 200 CCTTTGCTAT GAGAATCGTC GAAAATTGCT TGTGCAACTT GCCAAAGAGT 250 ATGATCTTAA CAGGACNCAG GTTCGCGATT TGATAAAGCA GTATTTGGGA 300 CTTGAGCTTC CTGGAACTGG AAGTGACAAT GCTGACTCAG AAAGAGGAGG 350 CATCTCTTTC TGCTTTCTAC CGCATTGANA GGAACTTGAA GACNTGCTCT 400 CNAGCCCATG TATGAANTGC TATTTGAGCG GCTTAATACG CNTCCCGGAG 450 GGTTGAAGTT CTTGTCTATT CTTTCGAGCT GATATCTTTA TCCATTCTCG 500 CANAAAAATA ATCTGGCGTC TTTGCNAACA TTGGATTCCC CATTCAAAGG 550 AGAAACTTAN TNCGTTGGTT AATCCCCCTG CCTTANNAGC TCCNCCCCCA 600 TCNCTCGGAT GATTCTTCCT CCCTTTGCTG GGAAAAAATT GTNGCTTACT 650 AAGGCCGTGC TTCCCATCCA NCTATTCTTC TNGAT 685 (2) INFORMATIONFORSEQLDNO 27 (i) SEQUENCECHARACTERISTICS (A) LENGTH 668 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (xi) SEQUENCEDESCRIPTION SEQLD27 AAGTTCACCC AAGTATAAAG TCTATCTTAT TATATATACG TTTTCTACAA 050 TGTTTGACTA TTACTGATAT TANAANATCA GCTTAAGGAG CAACAAACAT 100 ATTATTACAT TATAATGACA ACAGTACATT GATAATCACT TTCCACTATA 150 GAAAACAACA AAATTAAAAG TGTGGACACA TCCGTTATTA CATTGCTACC 200 CGGCTATTCT GTTGTA T GAGGTTCCTT CAGTGGCTCA ACGTAACGGG 250 AAAGTACATT AAAANTATGG ATATGCCCTG TNCTGAAATA TGACTGAAAA 300 TAATCTTCAA TGTTGCCCAA TCTGTAAACA TAGTTCACCA TGATACCTCC 350 ACTTTGATNA AGGCL ΎTAT CTGATCGATC AGCCATCCNA TTAATTCTCT 400 CAACCATTGC TCCATTCTGT NAGTTGAAAA TTTGCAACAG AATCCANAAC 450 TTTGCCTCTC TTTTTCTCTT GCAAAAANGT ANCTGGCACA CAATCCCATT 500 AAAAAGGGGT TTTTAGAACT GAAAACCAAT TTATCANAAC TTTGTTCCCT 550 CCCGGGTTTG CTGAANTTCC GTAAATTGAN CATCCCTCCA TGCCGTTTTT 600 TCCCCNTGGG TGAATTCAAA AAACCTNCTC NAAAANTNTT TCTAAAACNG 650 GCGCGGGGCC ATNCATTT 668 (2) INFORMATION FOR SEQ LD NO:28 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 522 (B) TYPE. cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHER INFORMATION O-methyl transferase (xi) SEQUENCE DESCRIPTION SEQ LD:28 NTNNGGGGGT TGGGGNTCTN GAAGGCAAAA GATTCGGTCA GGACAAGGTC 50 CTCGTCGAGA GCTGGTATCA TTTGANGGAT GCAGTTCTTG ATGGTGGGAT 100 TCCATTTAAC AAGGNCTATG GCATGACTGC ATTTGATTAC CATGGNAACT 150 GACCCTAGCA TTCAACAAGG TCTTCAACAA GGGAATGGCT GACCACTCCA 200 CCATTACCAT GCANGTAAAA TCCTTGTAGT ACTTACAAAG GCTTCGAGGG 250 CCTCAAATCC ATCGTTGTAT GTCGGTGGGCG GNACCNGAGC TGTGGNGGAA 300 CATNATCGCT TCCCNAGTTN CCCTTCGCAT CAAGGGTCAT CANCCTTTCG 350 ACTTGCCCTC AATCTTANTC GAANGCATTC CTCCNTCAAT TATCCTNNNT 400 GTTTCCANCC ANGTTGGGAT GNGGGGANAA TCTTCTGGCN ANNTCTTACC 450 CAATTNNGGN ANNCTTCCAT TCTTTCCCAT TTNAGTTCNT NTTTTNCTCA 500 ACCTAACTTG NCGNTCCNTC GN 522 (2) INFORMATIONFORSEQLDNO:29: (i) SEQUENCECHARACTERISTICS: (A) LENGTH: 445 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (ix) FEATURES (D) OTHERINFORMATION: Acyl carrier protein (xi) SEQUENCEDESCRIPTION: SEQLD:29: ATGGCCACCA CCACAGGAGC TGCTTCTTCG ATCTCACTCC GCTCTCGCCT 50 TCACCAGAAT CTTGCATTGT CCAGGGTCAA TGGTCTTAAG CCAGTTTCAC 100 TGTCTGGTAA TGGAAGAAGT TCTCTTTCTT TCGGGTTACA GCAGCGTTCA 150 GTACGGCTTC AGATTTGCTG CGCGGCCAAA CCAGAGACAG TGGACAAGGT 200 GTGCCAGATA GTTAGAAAGC AACTTGCATT ACCAGATGAC TCAGCAGTTT 250 CTGGAGAGTC AAAATTTTCT GCACTTGGAG CTGATTCTCT TGATACGGNN 300 GGAGATTGTG ATGGGACTTG AGGAGGAATT GGGTATTAGT GTGGNNGAGG 350 AGAGTGCTCA GAGCATTGAA CTTNTNCAAG NTGCTGGGGT CTTTTCNANA 400 AGNNCNATNG NAAGACCAGG NTTIGGAGGA GGANTNANAA ACAAG 445 (2) INFORMATION FOR SEQ LD NO 30 (l) SEQUENCE CHARACTERISTICS (A) LENGTH 562 (B) TYPE cDNA (C) STRANDEDNESS Linear (D) TOPOLOGY Single (ix) FEATURES (D) OTHERINFORMATION Elongation factor 2 (xi) SEQUENCE DESCRIPTION SEQLD30 GGATCATCCC TTGGNCCAAT ACGACCATCA TCAATGGNCT CAGGAAGACC 50 TTCCTCCAAC GGGNGTGCTT CCATGTACAG ACGGTTGTGC TTGTTGGGAG 100 ACTTGCTCAT CACAGTACGG NAGGNCTTCT CAAGGACTGT CTCACGGNAG 150 GACACAACAG GATCAGATTT TACAATTTCC GCTCCACCCA TAAAATCATC 200 TTGNAGATCC NTCANGNAGA TCTCAAGGTG AAGTTCACCA GCTCCAGCAA 250 TGATGTGCTC TCCAGACTCC TCAATGGTAC AGACACCCAT AGGGATCGGG 300 TCTTAGCCAG ACGTTTCAGC CCTTCAACAA GCTTGGGGAA GGATCAAGAA 350 GCANCCTTAC GNTTGAACAG CAACACGCAC AACAGGGTTG AGACAGGAGA 400 ACTTCATTGC ACGAATGGGG GGAGCATCTT GTTNCCCTCT CATTTGGTCA 450 AGGTAGCATT CTTGGGTGGA TTGAACTTNT TCCCAGACCA ACCAAGGGNA 500 C-AAGTTTTTA CCACAGGGGA ACATCCTCAA CAGTTTCNTT GTTTCTTTTC 550 CCCATCCAGG TT 562 (2) INFORMATIONFOR SEQ LD NO 31 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 490 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHER INFORMATION Auxin-induced mRNA (xi) SEQUENCEDESCRIPTION SEQLD31 ATCGACTGCA TTAAGTTGCT AGAAGTGGAG CTTGGTGACA AGCCTTTCTT 50 TGGCGGTGAG ACCCTCGGAT TTGTGGACGT GACGCTCGNT CCTTTCTATT 100 CCTGGTTCTC TGTGTATGAG AAATACGGCA ACTTCAGCAT TGCGCCAGAG 150 TGCCCAAAGT NCATGGCTTG GGTTAAGAGG TGTATGGAGA AGGAGAGTGT 200 GTCAAAGTCT CTTCCTGACC AGGACAAGGT CTGTGGCTTN GTTGCCGAGA 250 TGANGAAGAA GCTTGGAGTT GAGTAGATGT GATCAATGTC ATNTTGATCA 300 TGTCTTTGTT TTAGCCCCAA GATTCANCCT CGTTTTGGGT TGCTTGTATT 350 TTTCAATAAA ATTGGGGGAC TTGGACCAAG CCCTCCAATA GTAGGAAGCA 400 CTCTTTCNGT GCCTCTTGGT CCNGTITTTC TTCNGNTAAN CCTNTNTGCA 450 GCTAAAATTC ACCGNATTNC TGNTTTCCTT NTATNGCCAA 490 (2) INFORMATION FOR SEQ LD NO 32 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 483 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHER INFORMATION Cysteine (thiol) proteinase (xi) SEQUENCE DESCRIPTION SEQ LD 32 GGATCTCCTC CTCCTCTCTC TCCTTCTCCT CCTCTCCTCC GCCGTCGCCT 50 CCACCGTAAC CGACGCCGGC GATCCTCTCA TACGACAAGT CGTACCGGGC 100 GCGGCCGAGG ATGACGAGCT CCTCCACGCG GAGCGTCACT TCTCGAACTT 150 CAAAGCCACG TTCGGAAAGA GCTACGCGAG CCAGGAGGAG CACGACTACA 200 GGTTCCGGCG TATTCAAGGN CAACTCCGCC GGGCGAAGAG GCACCAGGGG 250 CTTGGACCCC ACCGCCGTGC ACGGTGTCAA CGAAATCTCC GATCTCACTC 300 CCAAGGAGTT TCGNCGGGAA TTTCCTCGGG CTTAAGAAGG GGTCGGANTT 350 CGGGTTACCG GCCGACGGTT AAAAAAGGGG CCNGATNCCT NCCGGANGAA 400 TTANCTTCCC CACCCANTTT TGGNNTTGGG GNGAAAAAAG GNGCCCGNCN 450 AAGNCGGNGG AANGGNCAAG GGGGAAATNG GGT 483 (2) INFORMATIONFORSEQ LDNO.33 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 520 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Cellulase (endo-( 1 ,4)beta-n-glucanase (xi) SEQUENCE DESCRIPTION SEQ LD 33 ACGGTGGGGG GACAGACTAC CTCCTGAAGG CCACGGGGGT TCCTGGCGTC 50 GTCTTCGTCC AAGTCGGCGA CCCATACTCC GATCACAACT GCTGGGAGGA 100 GGCCGGAAGT ACATGGTACA CACGCCGCAC GGTGTACAAA ATCGACCACA 150 ACAACCCGGG ATCCGACGTG GNAGGTGTAA ACCGCAGTTC GTGCTCGCCG 200 TCGCCTCTAT CGTTTTCAGG TCACGTGACC CCGCTTACTC GNAGNACTGC 250 TTCTCAATCG GAGCCGTTAA GGTTTICGAG TTCGCTGATA CCCACCGTGG 300 TGTGTTCAGA TCCAGCCTCA AAAACGCCGT TGTGCCCCTT TTTTACTGTG 350 NAANGTCAAA CGGNTTTCCA GGGATNAATT TACTNTTNGG GGAGGNAGCG 400 TTTGTTTGGN ACAAAGGTGG TCTATTNGGC NGGAGTACAA GTAGTATTNT 450 CATTGTGNTN AATCGGANGN CTATTTTGGG GGAGNTTTNA GGNTNCCMT 500 TAANGAANTT TGNNTGGGCT 520 (2) INFORMATIONFORSEQLDNO 34 (i) SEQUENCECHARACTERISTICS (A) LENGTH 695 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Pyruvatedecarboxylase (xi) SEQUENCEDESCRIPTION SEQLD34 GGCATCTTTT CACTCGAAGT CTCAATCTTT CATCACAAAC ATTCCCATTT 50 GATCACAAAA AAGTTTCAAC CTTTAAACCT CCATGGACAC CAAGATTGGC 100 TCCATCGACG TCTGCAAAAC CGAGAACCAC GACGTCGGTT GTTTACCAAA 150 CAGCGCCACC TCCACCGTTC AAAACTCAGT CCCTTCGACC TCCCTCAGCT 200 CCGCCGACGC CACCCTCGGC CGCCACCTGG CACGCCGCCT CGTTCAAATC 250 GGCGTCACCG ACGTCTTCAC CGTCCCCGGC GACTTCAACT TGACCCTTCT 300 CGACCACCTC ATCGCCGAGC CCGGCCTCAC CAACATTGGC TGCTGCAACG 350 AGCTCAACGC CGGGTACGCC GCCGACGGCT ACGCGCGGTC GCGTGGCGTC 400 GGCGCCGTTG CGTGGTGACT TTCACTGTTG GTGGACTGAG TGTGCTGAAC 450 GCGATCGCCG GCGCGTTATA GTGAGAATTT GCCGGTGATT TGTATTGTTG 500 GTGGGCCCCA ACTTCTAATG ATTATGGGAC TAACCGGATT CTTCACCATA 550 CTATTGGGTT GCCGGACTTC ANTTCAAGAA CTCCGGTGGT TTCAAGAACN 600 TGACTTGCTT TTCAGGCTGT GGGTGAATAA TTCTTGGAAG AATGCACATG 650 AATTTGCTTG AATACNGCAA TTTTCAATNG CNTTNGAAAN AAAAC 695 (2) INFORMATIONFORSEQLDNO35 (i) SEQUENCECHARACTERISTICS (A) LENGTH 695 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Chalconereductase (xi) SEQUENCEDESCRIPTION SEQLD35 GGCCCAAATC CCAGAAGTGG TTCTTGAATC CTCCAACGGC CGCAGAACCA 50 TGCCTGTGCT TGGATTCGGC ACAGCATCCA ACAATTTACA ACCGGAGGTT 100 TTGATAGAAG CTGTTCTTGA GGCCATCAAG CTTGGTTACC GACACTTCGA 150 CACTGCTTCC ATTTACGGCT CCGAGCAGAC TCTAGGAGTA GCCATTGCCC 200 AAGCGCTCAA ACTCGGCCTC GTGGCTTCTC GTGACGAGCT CTTCATCACT 250 TCCAAGCTTT GGCCTAATGA TGGTCACCCC AACCTGGTTA TTCCTGCTCT 300 CAAGAAAATC GCTTCAGAAT CTTGAGTTGG AGTACCTTGA TTTGTATCTG 350 ATACACTGGC CCATCAGTGC CAAGCCTGGG AAAGTTGAGT CACGCACTAG 400 AGGGAGAAGG ACCAAATGCC GATGGACTTC AAGGGTGTGT GGGCAGACAT 450 GGAGGAAGCT CAGAGACTTG GCCTCACCAA ATCCATTGGG AATCAGCAAT 500 TTCTCTACCA AAAAGACTCA GAATTTGCTC TCCTTTGGCT ACTATTCCTC 550 CGTCAGTCAA TCAANTTTAA NATGANTCCA TTTTGGCAAC AGAAGAACCT 600 CAAAAACTTC TGCAAGGCCA GTGGTATAAT TTGTGACTGG CTTCTCCCCA 650 TTGGGTGCCA TNNGAACCAN TTGGGGGCAC CAATCATGTT CTCNA 695 (2) INFORMATIONFORSEQLDNO 36 (i) SEQUENCECHARACTERISTICS (A) LENGTH 765 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Proteinkinase (xi) SEQUENCEDESCRIPTION SEQLD36 GGGCANANCG TGTTGTGGGA ACTGGGTCAT TTGGAATTGT ATTCCANGCG 50 AAATGCTTGG AAACTGGTGA GACTGTGGCC ATAAAGAAGG TTTTACAGGA 100 CAGAAGGTAT AAGAACAGGG AACTTCAATT GATGCGCGTA ATGGATCATC 150 CAAATGTGAT TTGTTTGAAG CATTGTTTCT TCTCTACAAC AAGCAAAAAT 200 GAGC-TTTTTC TCAATTTGGT TATGGAATAT GTTCCGGAAA CTATGTATCG 250 GGTTATAAAG CATTACAGCA ATGCAAACCA GAAAATGCCC CTTGTCTATG 300 TCAAACTTTA CATGTNCCAC ATTTTCAGAG GGCTGGCTTA CATACACACC 350 GTTCCTGGAG TTTGCCATAN ANATTTGAAN CCTCCAAATT TATTGGTTGA 400 TCCTCTTATT CACCANGTCA AGCTTTGTTG ATTTTGGAAG TGCCAAAATG 450 CNGGTGAAAG GNGAAACAAA CATANCATAC CTATGTTTCA CGTTTCTATC 500 NGGCTCCNCG AAACTAATTT TTTGGTGCCN CCNGATTATA CCACTTCCCA 550 TTGATATCTG GTCNGCTGGC TGTGTCCTAA NCAAAACTTC CTTTTGGGCC 600 CCCCTTTGTT TCCCTGGAAA AAAATGCCAT NGAACCACCT GTTAAAAATC 650 NTTCCNGGTT CNGGGGAACA CCNCNCCNTT CAAAAAATCC CCNTTTTGAA 700 TCCCCANTTN TACCAAATTC CCGGTTTCCN CCGAAAAAAN CCCNCCCTTT 750 GGNNNAAGGT TTTCC 765 (2) INFORMATION FOR SEQ LD NO 37 (i) SEQUENCE CHARACTERISTICS (A) LENGTH 772 (B) TYPE cDNA (C) STRANDEDNESS Single (D) TOPOLOGY Linear (ix) FEATURES (D) OTHERINFORMATION Auxin-relatedgene (xi) SEQUENCEDESCRIPTION SEQLD 37 GGAGAAACCT CTGCCCTTTA AACTTTACTT TTGCAATACA CCGTCTAACA 50 ATGGCTGCAG CTCCAAGTGA GTCCATACCC TCTGTAAATA AGGCCTGGGT 100 CTATTCAGAG TATGGAAAAA CTGCTGATGT TCTCAAGTTT GATCCAAGTG 150 TGGCTGTTCC TGAAATTAAA GAGGATCAGG TGCTGATCAA GGTTGTTGCT 200 GCTTCTCTTA ACCCAGTTGA TTTTAAGAGG GCTCTTGGTT ACTTCAAGGA 250 CACTGACTCT CCCCTACCTA CAATTCCAGG GTATGATGTA GCTGGTGTGG 300 TGGTAAAGGT AGGAAGCCAA GTAACCAAGT TTAAGGTGGG GGATGAAGTG 350 TATGGGGATC TCAATGAAGA CAGCATTGGT GAAACCCAAC AAGGTTTGGG 400 TCTTTGGCAG AGTACACTGC TGCAGATGAA AGANTATTGG CTCACAAACC 450 CAAAAACCTG AGCTTTATTG AAGCTGCTAA CCTTCCCTTG GCTATTGAAA 500 CTGCCCATGA AGGGCTTGAA AGAACTGAAC TTTCTGCTGG TAAATCCGTC 550 CTTGTTTTGG GAAGCGCTGG GGGTNTTGGA ACACATATTA TCANCTTGCC 600 AAAGCATGTT TTTGGTGCTT CCCAANTAAC NNCTACTGCA ANCACTAAAA 650 AACCGGAATT TGTTGAAAAA CCTGGGTNCT GATTTGGCTA CCAATTACCC 700 CANGAAAACT TCCAAGAACT GCCCAAAAAA TTGAATTTTN TTTTTNANGC 750 CNTTNGGGAA ANNAANAAGG GT 772 (2) INFORMATIONFORSEQLDNO:38: (i) SEQUENCECHARACTERISTICS: (A) LENGTH: 773 (B) TYPE: cDNA (C) STRANDEDNESS: Single (D) TOPOLOGY: Linear (ix) FEATURES (D) OTHER INFORMATION: Sucrose transporter (xi) SEQUENCEDESCRIPTION: SEQ D:38: CATTGGCGAT CACGTACAGT GTTCCATATG CCTTGATTTC TTCTCGTATC 50 GAGTCTTTGG GACTTGGCCA AGGCTTATCA ATGGGTGTAC TGAATCTGGC 100 AATCGTAGTA CCACAGGTGC TGGTATCCCT GGGAAGTGGA CCATGGGATC 150 AGCTATTTGG TGGTGGAAAC TCTCCAGCCT TTGCGGTTGC AGCAGTTGCA 200 GCCTTAGCAA GTGGGCTGGT GGCCATCTTG GCTATTCCAC GTTCTATTCC 250 ACAGAAGCCT ANATCTTTCA CATGAGGTAT TTTGTTGTAT CTACTTTTTA 300 CCCAACTTTG TCACAGAAAT ACAAAACCTC CATAGATAGT GAGAATTTGT 350 AAATATCTTT TGTTACGTGT TAGCTATTTC TCAATACACT CATTTACCAG 400 AGGTTTCTTT AGTTCTGGAA ATTTCTCTCT TTCCCTTTTT GTCGTTTTAG 450 ATGCTTTAAT AAAGAAAGGC CTGGCAGCGA TTATATCAAA GTTGANCTGA 500 ATATCTGTGT TGAAGTGCTT CCGTTCAACA ATTTATAGTT CTCAATTTCT 550 ACAATATTTT AAATCAGAAC TGTCCCCTGG TTGGACCCTA ATGGAATCCA 600 TATGTTGGAA CCATAATCTC AATTANGCAT CCTGCCTCAA TTCCNCAATG 650 GTGTTTTCAN AANTGTTGAN GAAACNANTT NNTCCAAAAA GTTGATGGTG 700 TTTTTCCCAA ATGCCNGGCT ACNCCACCAA NNTTGANGTT NGGTACNCCA 750 AATTGAATNA AGTTATTACC CAC 773

Claims: CLAIMS 1 A vector for use in the genetic transformation of strawberry cells, comprising a promoter sequence, a regulation sequence and a transcription termination sequence, in which the regulation sequence comprises the coding region, or a fragment of at least 10 bases thereof, of a strawberry protein selected from O-methyl transferase, acyl carrier protein (ACP), elongation factor, auxin-induced gene, cysteine( thiol) proteinase, cellulase, starch phosphorylase, pyruvate decarboxylase, chalcone reductase, protein kinase, auxin-related gene, sucrose transporter, meristem pattern gene, or selected from a strawberry protein with homology to transcribed sequence, T45086, transcribed sequence accession number L36159 or transcribed sequence accession number T45902, or selected from a strawberry protein of unknown homology encoded by one of the StrawRipe sequences A to K 2 A vector according to claim 1, wherein the regulation sequence comprises a sequence selected from SEQ LD NO:l . to SEQ LD NO.38 , and fragments thereof with at least 10 bases 3 A vector according to claim 1 or 2, wherein the regulation sequence is aligned for antisense expression 4 A vector according to claim 1 or 2, wherein the regulation sequence is aligned for sense expression 5 A vector according to any preceding claim, wherein the regulation sequence fragment comprises at least 35 bases 6 A method for genetic modification of a strawberry comprising inserting a vector as claimed in any preceding claim into the genome of a strawberry plant 7 Propagation material for a strawberry plant which plant is progeny of a strawberry plant which has been modified by a method according to claim 6. 8 Strawberry fruit of a strawberry plant grown from propagating material according to claim 7. 9 Strawberry fruit according to claim 8, with regulated ripening in comparison with unmodified fruit. 10 A gene regulation sequence selected from SEQ LD NO.1 : to SEQ LD NO 38 , and fragments thereof with at least 10 bases.

Inventors: MANNING KENNETH, MANNING, KENNETH
Applicants: HORTICULTURE RES INT, MANNING KENNETH, HORTICULTURE RESEARCH INTERNATIONAL, MANNING, KENNETH
Application year: 1997
Main classification: C12N15
IPC codes: C07K14, C12N9, C12N15