ANTIVIRAL 2,4-PYRIMIDINEDIONE DERIVATIVES AND PROCESS FOR THE PREPARATION THEREOF

Abstract: 6-aryloxy and 6-arylcarbonyl 2,4-pyrimidinedione derivatives of formula (I) having high antiviral activity against HIV-1 and low toxicity are useful for treating AIDS, wherein R<1> is hydrogen or a C1-10 alkyl group optionally having a substituent selected from the group consisting of aryl, hydroxy, C1-10 alkoxy and C2-5 alkylcarbonyloxy groups; R<2> is hydrogen or a C1-10 alkyl group optionally having an aryl substituent; R<3> and R<4> are each hydrogen or a C1-3 alkyl group; and A is oxygen or a carbonyl group.

Description: ANTIVIRAL 2,4-PYRIMIDINEDIONE DERIVATIVES AND PROCESS FOR THE PREPARATION THEREOF Field of the Invention The present invention relates to novel pyrimidinedione derivatives having 6-aryloxy or 6-arylcarbonyl substituents, which are useful as an antiviral agent, particularly for treating acquired imitiunodeficiency syndrome (AIDS), and pharmaceutically acceptable salts thereof. The present invention also relates to a process for the preparation of such derivatives and to a pharmaceutical composition containing same as an active ingredient. Description of the Prior Art Various compounds such as AZT ( 3 '-azido-3 ' -deoxythymi- dine), DDC (2 ' , 3 '-dideoxycytidine) , DDI (2 ' , 3 '-dideoxyino- sine) , D4T ( 3 ' -deoxy-2 ' , 3 ' -didehydrothymidine ) 3TC(lamivudine) , Nevirapine, Indinavir, Ritonavir and Saquinavir have been reported to have the ability, albeit limited, to inhibit the reproduction of AIDS virus. However, they are also known to cause undesirable side effects due to their toxicity as well as to induce the mutation of the virus, thereby increasing the resistance of the virus . In order to minimize such problems, therefore, many attempts have been made. For example, 2, 4-pyrimidinedione derivatives substituted with an alkoxymethyl group on the N- 1 position thereof have been reported in J. Med. Chem. , 35, 4713 (1992); J. Med. Chem. , 3!5, 337 (1992); J. Med. Chem. , 34 ./ 1508 (1991); J. Med. Chem., 34 , 1394 (1991); J. Med. Chem. , 3±, 349 (1991); Molecular Pharm., 3_9, 805 (1991); Tet. Lett.. 3_5, 4531 (1994); J. Med. Chem. , 38 , 2860 (1995); Nucleosides and Nucleotides, 14, 575 (1995); J. Med. Chem. , li, 2427 (1996); EP 0,449,726 Al; EP 0,420,763 A2; USP 5,318,972; and WO95/18109 Al to have improved activity against human immunodeficiency virus (HIV), while exhibiting lower toxicity. However, needs have continued to exist for compounds having higher potency against HIV while exhibiting a much lower toxicity. Summary of the Invention Accordingly, it is a primary object of the present invention to provide a novel compound having both superior antiviral activity against HIV and reduced toxicity. It is another object of the present invention to provide a pharmaceutical composition containing same. It is a further object of the present invention to provide a process for the preparation of said novel compound. In accordance with one aspect of the present invention, there are provided a novel 2,4-pyrimidinedione compound of formula(I) and pharmaceutically acceptable salts thereof: wherein: R 1 is hydrogen or a C V10 alkyl group optionally having a substituent selected from the group consisting of aryl, hydroxy, C.,_ 10 alkoxy and C 2 _ 5 alkylcarbonyloxy groups; R 2 is hydrogen or a C V10 alkyl group optionally having an aryl substituent; R 3 and R 4 are each hydrogen or a C,_ 3 alkyl group; and A is oxygen or a carbonyl group. Detai led Description of the Invention Among the compounds of formula(I) of the present invention, the preferred are those wherein R 1 is methyl, hydroxymethyl, acetoxymethyl or phenyl. Among the preferred compounds of the present invention, the more preferred are those wherein R 2 is ethyl or isopropyl . Among the more preferred compounds of the present invention, the most preferred are those wherein R 3 and R A are each hydrogen or methyl . The 2, 4-pyrimidinedione compound of formula(I) may be prepared by silylating a compound of formula(II) to give a compound of formula(III) and coupling the compound of formula(III) with a compound of formula(lV), as shown in the following Reaction Scheme A: Reaction Scheme (A) (ID wherein : R 1 , R 2 , R 3 , R A and A have the same meanings as defined in formula(l) above; and X is halogen or acetoxy. In Reaction Scheme A, the silylation of the compound of formula(II) may be conducted under a conventional silylation condition. Preferably, it may be carried out by refluxing the compound and 1, 1, 1, 3, 3, 3-hexamethyldisilazane, which is used as a solvent and as a silylating agent, for 2 to 24 hours in the presence of a catalyst, e.g., chlorotrimethylsilane. The reaction between the compound of formula(III) and the compound of formula(IV) may be conducted in the absence or presence of a Lewis acid at a temperature ranging from - 20 to 100°C. Preferably, it may be carried out by stirring the reaction mixture at a temperature ranging from 0°C to room temperature. For example, a mixture of the compound of formula(III) and the compound of formula(IV) in a molar ratio of 1:0.5 to 1:2, preferably, 1:0.8 to 1:1.2 may be stirred in the presence of tin tetrachloride under a nitrogen blanket at room temperature for 1 to 24 hours. Suitable for use in this reaction is a nonpolar solvent, e.g., acetonitrile, dichloromethane or 1, 2-dichloroethane. The compound of formula(II) may be prepared in accordance with the procedure disclosed in PCT International Publication WO95/18109, as illustrated in Reaction Scheme B below. Reaction Scheme (B) Method (i) : A is C=0 Method (ii) : A is 0 (Il-b) wherein, R 2 , R 3 and R 4 have the same meanings as defined previously in conjunction with formula(I). In accordance with the method (i) in Reaction Scheme B, a compound of formula (V) is methoxylated using a known method disclosed in, e.g., Ber. , 52B, 869 (1919) and J. Med. Chem. , 7_, 808 (1964) to provide a compound of formula (VI) (Step (a)). Preferably, Step (a) may be carried out by reacting the compound of formula(V) with sodium methoxide at room temperature. The compound of formula(VI) is then subjected to a coupling reaction with an acetonitrile derivative in a polar solvent, e.g., dimethylformamide, in the presence of a strong base, e.g., sodium hydroxide, to provide a compound of formula(VII) (Step (b)). The compound of formula(VII) is reacted with a base, e.g., sodium hydride, in a polar solvent, e.g., dimethylformamide, to give a compound of formula(VIII ) (Step (c)). Then, the compound of formula(VIII ) may be hydrolyzed with an acid, e.g., hydrochloric acid, to provide a compound of formula (II-a). In the method (ii) of Reaction Scheme B, the compound of formula (V) is reacted with a phenol derivative in the presence of a base, e.g., sodium hydride, in a polar solvent, e.g., dimethylformamide, to provide a compound of formula (IX) (Step (e)). The compound of formula(IX) is treated with an alkali metal, e.g., sodium, and then reacted with benzyl alcohol to provide a compound of formula (X) (Step (f)). Then, the compound of formula(X) is hydrogenated in the presence of a palladium catalyst in an alcohol solvent, e.g., ethanol, to provide a compound of formula(II-b) (Step (g)). Exemplary compounds of formula(I) of the present invention which can be prepared in accordance with the methods described above are listed below: Furthermore, the present invention encompasses, within its scope, those pharmaceutically acceptable salts of the compounds of formula(I). Suitable pharmaceutically acceptable salts of the 2, 4-pyrimidinedione compounds ( I ) possessing strong antiviral activity against HIV may include alkali or alkaline earth metal salts, e.g. , sodium, potassium, magnesium and calcium salts thereof. The present invention also includes within its scope pharmaceutical compositions comprising one or more of the compounds of formula(I) and their above-mentioned salts as the active ingredient, in association with pharmaceutically acceptable carriers, excipients or other additives, if necessary. The pharmaceutical compositions of the invention may be formulated for administration orally or by injection. The composition for oral administration may take various forms such as tablets and gelatin capsules, which may contain conventional additives such as a diluent (e.g., lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and/or glycine), a lubricant (e.g., silica, talc, stearic acid or its magnesium and calcium salts and polyethylene glycol). In the case of the tablet form, the composition may further comprise a binder (e.g., magnesium aluminum silicate, starch paste, gelatin, tragacanth, methyl cellulose, sodium carboxymethyl cellulose and polyvinyl picolidine) and optionally a disintegrant (e.g., starch, agar and alginic acid or its sodium salt), absorbent, colorant, flavor, sweetener and the like. The composition for injection may be an isotonic solution or a suspension. The composition may be sterilized and/or contain an adjuvant such as a preservative, stabilizer, wetting agent, emulsifier, a salt for controlling an osmotic pressure and/or a buffer solution, and other pharmaceutically effective materials. The pharmaceutical compositions can be prepared by a conventional mixing, granulating or coating method and may contain preferably about 0.1 to 75 %, more preferably about 1 to 50 % of the active ingredient of this invention. The unit dosage of the composition suitable for administering a person weighing about 50 to 70 kg may contain about 10 to 200mg of the active ingredient. The following Preparation and Examples are given for the purpose of illustration only and are not intended to limit the scope of the invention. In the Preparation and Examples, unless otherwise specified, the evaporation was conducted under a reduced pressure, preferably under a pressure ranging from about 15 to 100 mmHg. Preparation The compounds of formula(II) having one of the structures (A) to (H) shown below may be used in preparing the compound of formula(I) of the present invention. The compounds having the specified structures (A) to (H) were prepared in accordance with the procedures described in PCT Publication WO95/18109. (G) (H) The melting points and NMR data of the compounds of structural formulae (A) to (H) obtained as above are summarized in Table 1 below. Table 1 Example 1 : Synthesis of l-ethoxymethyl-5-ethyl-6-(3 ' ,5' dimethylphenoxy)-2,4-pyrimidinedione (Compound 1) 5ml of 1, 1, 1,3,3,3-hexamethyldisilazane and 5μl of trichlorotrimethylsilane (0.05mmol) were added to 260mg of the compound (F) (lmmol) obtained in the Preparation and the mixture was refluxed at 130 to 140°C for about 14 hours. Then, the solvent was removed under a reduced pressure and the residue was dissolved in 5ml of acetonitrile. To the resulting solution were added 93μl of chloromethylethylether (lmmol) and 100 μl of IM tin tetrachloride solution in dichloromethane (0. lmmol), and the mixture was stirred at room temperature for about 24 hours. Then, an excess amount of anhydrous sodium bicarbonate was added thereto, followed by stirring for about 30 minutes. The solvent was removed under a reduced pressure and the residue was subjected to column chromatography using a mixture of ethylacetate and hexane (1:2) as an eluent to afford 207 mg (yield 65 %) of the title compound as a white solid. Melting Point: 139-140°C 1 H-NMR (200 MHz, CDC1 3 ) δ 0.92(3H, t, J=7.5Hz), 1.09(3H, t, J=7.0Hz), 2.19(2H, q, J=7.5Hz), 2.28(6H, s), 3.57(2H, q, J=7.0Hz), 5.20(2H, s), 6.56(2H, s), 6.75(1H, s), 9.04(1H, s). Example 2 : Synthesis of l-ethoxymethyl-5-isopropyl-6- (3 ' , 5 '-dimethylphenoxy)-2, 4-pyrimidinedione (Compound 2) The procedure of Example 1 was repeated except that the compound (H) obtained in the Preparation was used in place of the compound (F) to afford 145 mg (yield 44 %) of the title compound as a white solid. Melting Point : 134-135°C 1 H-NMR (500MHz, CDC1 3 ) δ 1.10(3H, t, J=7.0Hz), 1.13(6H, d, J=7.0Hz), 2.30(6H, s), 2.79(1H, m) , 3.55(2H, q, J=7.0Hz), 5.18(2H, s), 6.56(2H, s), 6.76(1H, s), 8.92(1H, s). Example 3 : Synthesis of l-ethoxymethyl-5-ethyl-6-phenoxy- 2,4-pyrimidinedione (Compound 3) The procedure of Example 1 was repeated except that the compound (A) obtained in the Preparation was used in place of the compound (F) to afford 86mg (yield 30 %) of the title compound as a white solid. Melting Point : 130-131°C 1 H-NMR (200MHz, CDCl 3 ) δ 0.93(3H, t, J=7.3Hz), 1.09(3H, t, J=7.0Hz), 2.21(2H, q, J=7.3Hz ) , 3.58(2H, q, J=7.0Hz), 5 . 25 ( 2H , s ) , 6 . 97 -7 . 45 ( 5H , s ) , 8 . 56 ( 1H , s ) . Example 4 : Synthesis of l-ethoxymethyl-5-isopropyl-6- phenoxy-2,4-pyrimidinedione (Compound 4) The procedure of Example 1 was repeated except that the compound (G) obtained in the Preparation was used in place of the compound (F) to afford 95 mg (yield 31 %) of the title compound as a white solid. Melting Point : 137-138°C " Η-NMR (200MHz, CDC1 3 ) δ 1.04(3H, t, J=7.0Hz), 1.13(6H, d, J=7.0Hz), 2.78(1H, m) , 3.53(2H, q, J=7.0Hz), 5.17(2H, s), 6.94-7.39(5H, m) , 8.36(1H, s). Example 5 : Synthesis of l-benzyloxymethyl-5-ethyl-6-(3 ' , 5 '- dimethylphenoxy)-2,4-pyrimidinedione (Compound 5) The procedure of Example 1 was repeated except that chlorobenzylether was used in place of chloromethylethylether to afford 130 mg (yield 34 %) of the title compound as a white solid. Melting Point : 146-150°C 1 H-NMR (200MHz, CDC1 3 ) δ 0.93(3H, t), 2.14(2H, q), 2.27(6H, s), 4.63(2H, s), 5.28(2H, s), 6.55(2H, s), 6.76(1H, s), 7.26(5H, s). Example 6 : Synthesis of l-benzyloxymethyl-5-isopropyl-6- (3 ' ,5 '-dimethylphenoxy)-2,4-pyrimidinedione (Compound 6) The procedure of Example 1 was repeated except that the compound (H) obtained in the Preparation and chloromethylbenzylether were used in place of the compound (F) and chloromethylethylether, respectively, to afford 145 mg (yield 37 %) of the title compound as a white solid. Melting Point : 150-152°C 1 H-NMR (500MHz, CDC1 3 ) δ 1.12(6H, d, J=7.0Hz), 2.27(6H, s), 2.78(1H, m), 4.61(2H, s), 5.24(2H, s), 6.52(2H, s), 6 . 75 ( 1H , s ) , 7 . 20 ( 2H , d, J=7 . 0Hz ) , 7 . 28- 7 . 33 ( 3H , m ) , 8 . 54 ( 1H , s ) . Example 7 : Synthesis of l-benzyloxymethyl-5-isopropyl-6- phenoxy-2, 4-pyrimidinedione (Compound 7) The procedure of Example 1 was repeated except that the compound (G) obtained in the Preparation and chloromethylbenzylether were used in place of the compound (F) and chloromethylethylether, respectively, to afford 190 mg (yield 52 %) of the title compound as a white solid. Melting Point : 118°C 1 H-NMR (200MHz, CDC1 3 ) δ 1.12(6H, d, J=7.0Hz), 2.78(1H, m), 4.60(2H, s), 5.25(2H, s), 6.92-7.37 ( 10H, m) , 8.72(1H, s) . Example 8 : Synthesis of 1-(2 ' -acetoxyethoxymethyl)-5-ethyl- 6-( 3 ',5 '-dimethylphenoxy)-2 , 4-pyrimidinedione (Compound 8) The procedure of Example 1 was repeated except that chloromethyl-2-acetoxyethylether was used in place of chloromethylethylether to afford 180 mg (yield 48 %) of the title compound a colorless oil. 1 H-NMR (500MHz, CDC1 3 ) δ 0.94(3H, t, 7.5Hz) , 2.03(3H, s), 2.20(2H, q, J=7.0Hz), 2.30(6H, s), 3.80(2H, t, J=4.5Hz), 4.11(2H, t, J=4.5Hz), 5.28(2H, s), 6.58(2H, s), 6.78(1H, s), 9.48(1H, s). Example 9 : Synthesis of 1-(2 '-hydroxyethoxymethyl-5-ethyl- 6-(3' ,5 '-dimethylphenoxy)-2, 4-pyrimidinedione (Compound 9) 150mg(0.4 mmol) of the compound obtained in Example 8 (Compound 8) was dissolved in 5ml of methanol and 3ml of aqueous ammonia was added thereto, and stirred at room temperature for 2 hours. Then, the reaction mixture was concentrated under a reduced pressure and the residue was subjected to column chromatography using a mixture of dichloromethane and methanol (15:1) as an eluent to afford 75 mg (yield 56 %) of the title compound as a white solid. Melting Point : 92-93°C 1 H-NMR (500MHz, CDCl 3 ) δ 1.09(3H, t, J=7.5Hz), 2.34(6H, s), 2.43(2H, q, J=7.5Hz), 3.74(4H, q, J=7.5Hz), 5.42(2H, s), 6.70(2H, s), 6.91(1H, s), 7.93(1H, s). Example 10 : Synthesis of 1-(2'-acetoxyethoxymethyl)-5- isopropyl-6-(3' ,5 '-dimethylphenoxy)-2 , 4-pyrimidinedione (Compound 10) The procedure of Example 8 was repeated except that the compound (H) obtained in the Preparation was used in place of the compound (F) to afford 150 mg (yield 38 %) of the title compound as a white solid. Melting Point : 115-117°C 1 H-NMR (500MHz, CDC1-.) δ 1.13(6H, d, J=7.0Hz), 2.03(3H, s), 2.30(6H, s), 2.75-2.80(lH, m) , 3.76(2H, t, J=5.0Hz), 4.09(2H, t, J=5.0Hz), 5.22(2H, s), 6.56(2H, s), 6.77(1H, s), 8.40(11., s). Example 11 : Synthesis of l-(2 '-hydroxyethoxymethyl)-5- isopropyl-6-(3' ,5'-dimethylphenoxy)-2, 4-pyrimidinedione (Compound 11) The procedure of Example 9 was repeated except that the compound obtained in Example 10(Compound 10) was used in place of Compound 8 to afford llOmg (yield 32 %) of the title compound as a colorless oily form. 1 H-NMR (500MHz, CDCl 3 ) δ 1.14(6H, d, J=7.0Hz), 2.31(6H, s), 2.77(1H, m), 3.62(2H, d, J=5.0Hz), 3.64(2H, d, J=5.0Hz), 5.24(2H, s), 6.58(2H, s), 6.78(1H, s), 8.62(1H, s). Example 12 : Synthesis of l-(2 '-methoxyethoxymethyl)-5- isopropyl-6-(3' ,5'-dimethylphenoxy)-2,4-pyrimidinedione (Compound 12) The procedure of Example 1 was repeated except that chloromethyl-2-methoxyethylether was used in place of chloromethylethylether to afford 184 mg (yield 51 %) of the title compound as a white solid. Melting Point : 80-83°C ' Η-NMR (500MHz, CDC1 3 ) δ 1.13(6H, d, J=7.0Hz), 2.30(6H, s), 2.78(1H, m), 3.31(3H, s), 3.41(2H, t, J=4.5Hz), 3.72(2H, t, J=4.5Hz), 5.23(2H, s), 6.50(2H, s), 6.76(1H, s), 8.75(1H, s). Example 13 : Synthesis of l-ethoxymethyl-5-ethyl-6-(3 ' , 5 '- dimethylbenzoyl)-2,4-pyrimidinedione (Compound 13) The procedure of Example 1 was repeated except that the compound (B) obtained in the Preparation was used in place of the compound (F) to afford 146 mg (yield 44 %) of the title compound as a white solid. Melting Point : 184°C 1 H-NMR (200MHz, CDCl 3 ) δ 0.82(3H, t, J=7.0Hz), 0.93(3H, t, J=7.3Hz), 1.97-2.37(8H, m) , 3.28-3.47 (2H, m) , 4.93(1H, d, J=10.3Hz), 5.40(1H, d, J=10.3Hz), 7.29(1H, s), 7.35(2H, s), 10.19(1H, s). Example 14 : Synthesis of l-ethoxymethyl-5-isopropyl-6- (3' , 5 '-dimethylbenzoyl)-2, 4-pyrimidinedione (Compound 14) The procedure of Example 1 was repeated except that the compound (D) obtained in the Preparation was used in place of the compound (F) to afford 172 mg (yield 50 %) of the title compound as a white solid. Melting Point : 120-122°C 1 H-NMR (200MHz, CDCl 3 ) δ 0.81(3H, t, J=7.0Hz), 1.13(3H, d, J=6.8Hz), 1.22(3H, d, J=6.8Hz), 2.29-2.40(7H, m), 3.30-3.47(2H, m) , 4.88(1H, d, J=10.3Hz), 5.41(1H, d, J=10.3Hz), 7.31(1H, ε), 7.56(2H, s), 8.85(1H, s). Example 15 : Synthesis of l-ethoxymethyl-5-ethyl-6-benzoyl- 2,4-pyrimidinedione (Compound 15) The procedure of Example 1 was repeated except that the compound (A) obtained in the Preparation was used in place of the compound (F) to afford 180 mg (yield 60 %) of the title compound as a white solid. Melting Point : 133-134°C 1 H-NMR (200MHz, CDC1 3 ) δ 0.78(3H, t, J=7.0Hz), 0.95(3H, t, J=7.3Hz), 2.02-2.29(2H, m) , 3.26-3.44(2H, m), 4.90(1H, d, J=10Hz), 5.43(1H, d, J=10Hz), 7.49-7.97 (5H, m) , 8.77(1H, s) . Example 16 : Synthesis of l-ethoxymethyl-5-isopropyl-6- benzoyl-2,4-pyrimidinedione (Compound 16) The procedure of Example 1 was repeated except that the compound (C) obtained in the Preparation was used in place of the compound (F) to afford 244 mg (yield 77 %) of the title compound as a white solid. Melting Point : 144-145°C 1 H-NMR (200MHz, CDC1 3 ) δ 0.74(3H, t, J=7.0Hz), 1.12(3H, d, J=6.8Hz), 1.21(3H, d, J=6.8Hz), 2.33(1H, m) , 3.21-3.42(2H, m), 4.83(1H, d, J=10.2Hz), 5.43(1H, d, J=10.2Hz), 7.49-7.97(5H, m), 8.85(1H, s). Example 17 : Synthesis of l-benzyloxymethyl-5-ethyl-6- (3 ', 5 '-dimethylbenzoyl )-2, 4-pyrimidinedione (Compound 17) The procedure of Example 1 was repeated except that chloromethylbenzylether was used in place of chloromethylethylether to afford 252 mg (yield 64 %) of the title compound as a white solid. Melting Point : 115-116°C 1 H-NMR (200MHz, CDCl 3 ) δ 0.96(3H, t, J=7.4Hz), 2.06-2.29(8H, m) , 4.41(1H, d, J=12.0Hz), 4.45(1H, d, J=12.0Hz), 5.07(1H, d, J=10.5Hz), 5.44(1H, d, J=10.5Hz), 6.96-7.51(8H, m) , 10.32(1H, s). Example 18 : Synthesis of l-benzyloxymethyl-5-isopropyl-6- ( 3 ', 5 '-dimethylbenzoyl)-2, 4-pyrimidinedione (Compound 18) The procedure of Example 1 was repeated except that the compound (D) obtained in the Preparation and chloromethylbenzylether were used in place of the compound (F) and chloromethylethylether, respectively, to afford 270 mg (yield 66 %) of the title compound as a white solid. Melting Point : 68-70°C 1 H-NMR (200MHz, CDC1 3 ) 1.13(3H, d, J=6.8Hz), 1.23(3H, d, J=6.8Hz), 2.06-2.40(7H, m), 4.40(1H, d, J=12.0Hz), 4.46(1H, d, J=12.0Hz), 5.01(1H, d, J=10.4Hz), 5.42(1H, d, J=10.4Hz), 6.94-7.53(8H, m) , 8.35(1H, s). Example 19 : Synthesis of l-benzyloxymethyl-5-isopropyl-6- benzoyl-2, 4-pyrimidinedione (Compound 19) The procedure of Example 1 was repeated except that the compound (C) obtained in the Preparation and chloromethylbenzylether were used in place of the compound (F) and chloromethylethylether, respectively, to afford 210 mg (yield 55 %) of the title compound as a white solid. Melting Point : 184°C ■ Η-NMR (200MHz, CDC1 3 ) δ 1.16(3H, d, J=6.7Hz), 1.26(3H, d, J=6.7Hz), 2.41(1H, m) , 4.38(1H, d, J=12.1Hz), 4.47(1H, d, J=12.1Hz), 5.02(1H, d, J=10.6Hz), 5.46(1H, d, J=10.6Hz), 6.96-7.99(10H, m), 8.74(1H, s). Example 20 : Synthesis of l-(2 '-hydroxyethoxymethyl)-5- isopropyl-6- ( 3 ' , 5 '-dimethylphenoxy) -2 , 4-pyrimidinedione (Compound 20) The procedures of Examples 8 and 9 were repeated except that the compound (D) obtained in the Preparation was used in place of the compound (F) to afford 90 mg (yield 25 %) of the title compound as a white solid. Melting Point : 145-151°C 1 H-NMR (500MHz, CDCl 3 ) δ 1.13(3H, d, J=7.0Hz ) , 1.21(3H, d, J=7.0Hz), 2.36(1H, m) , 2.40(6H, s), 3.46-3.49( 3H, m), 3.55(1H, m), 5.09(1H, d, J=10.5HZ), 5.19(1H, d, J=10.5Hz), 7.34(1H, s), 7.57(2H, s), 8.40(1H, s). Example 21 : Synthesis of 1-(2 '-methoxyethoxymethyl )-5- isopropyl-6-( 3 ' ,5 ' -dimethylbenzoyl ) -2, 4-pyrimidinedione (Compound 21) The procedure of Example 1 was repeated except that the compound (D) obtained in the Preparation and chloromethyl-2- methoxyethylether were used in place of the compound (F) and chloromethyl-2-methoxyethylether, respectively, to afford 170 mg (yield 45 %) of the title compound as a white solid. Melting Point : 97-99°C 1 H-NMR (200MHz, CDC1 3 ) δ 1.12(3H, d, J=7.0Hz), 1.20(3H, d, J=7.0Hz), 2.35(1H, m), 2.40(6H, s), 3.12(1H, m) , 3.20(3H, s), 3.22(1H, m), 3.49-3.56 ( 2H, m) , 4.96(1H, d, J=10.5Hz), 5.38(1H, d, J=10.5Hz), 7.31(1H, s), 7.56(2H, s), 8.94(1H, s) . Antiviral Activity and Toxicity Test The in vitro anti-HIV-1 assays were based on the inhibition of the virus-induced cytopathic effect in MT-4 cells as described in J. Med. Chem, 34, 349 (1991). First, MT-4 cells were suspended in a culture medium at a concentration of 1 x 10* cells/ml and infected with 500 TCID 50 (50% cell culture infective dose)/well of HIV-1. Immediately after the virus infection, 100 μl of the cell suspension was brought into each well of a flat-bottomed microtiter tray containing various concentrations of the test compounds(1) to (21). After incubating for 4 or 5 days at 37 °C, the number of viable cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method, as disclosed in J. Virol. Methods, 20, 309 (1988) . The cytotoxicity of the compounds of the present invention was assessed in parallel with their antiviral activity. It was based on the viability of mock-infected host cells as determined by the MTT methods (see J. Virol. Methods, .20 . , 309 (1988)). AZT was employed as a comparative compound. The results of the tests are shown in Table 2. Table Foot note: * CD ■ Cytotoxic concentration that causes death of ** MT-4cells by 50 % ED 50' Effective concentration for the inhibition of the *** proliferation of HIV by 50% S.I Selectivity index = (CD 50 /ED 50 ) As the above results show, the novel antiviral 6- aryloxy and 6-arylcarbonyl 2,4-pyrimidinedione derivatives of the present invention possess high antiviral activity against HIV-1 and at the same time show high selectivity indices, i.e., low toxicity. The inventive compounds can therefore be used as a drug for treating AIDS. While the invention has been described with respect to the specific embodiments, it should be recognized that various modifications and changes may be made by those skilled in the art to the invention which also fall within the scope of the invention as defined by the appended claims.

Claims: What is claimed is: 1. A compound of formula(I) and pharmaceutically acceptable salts thereof: wherein: R 1 is hydrogen or a C,_ 10 alkyl group optionally having a substituent selected from the group consisting of aryl, hydroxy, C.,_ 10 alkoxy and C 2 _ 5 alkylcarbonyloxy groups; R 2 is hydrogen or a C v10 alkyl group optionally having an aryl substituent; R 3 and R 4 are each hydrogen or a C 1 _ 3 alkyl group; and A is oxygen or a carbonyl group. 2. The compound of claim 1 wherein R 1 is methyl, hydroxymethyl, acetoxymethyl or phenyl. 3. The compound of claim 2 wherein R 2 is ethyl or isopropyl . 4. The compound of claim 3 wherein R 3 and R A are each hydrogen or methyl . 5. The compound of claim 1, which is selected from the group consisting of: l-ethoxymethyl-5-ethyl-6- ( 3 ' , 5 ' -dimethylphenoxy) -2,4- pyrimidinedione; l-ethoxymethyl-5-isopropyl-6- ( 3 ' , 5 ' -dimethylphenoxy) -2 , 4- pyrimidinedione; l-ethoxymethyl-5-ethyl-6-phenoxy-2, 4-pyrimidinedione; l-ethoxymethyl-5-isopropyl-6-phenoxy-2,4-pyrimidinedione; l-benzyloxymethyl-5-ethyl-6- ( 3 ' , 5 ' -dimethylphenoxy) -2,4- pyrimidinedione; l-benzyloxymethyl-5-isopropyl-6-(3' ,5 '-dimethylphenoxy)-2, 4- pyrimidinedione; l-benzyloxymethyl-5-isopropyl-6-phenoxy-2,4-pyrimidinedione; 1-(2 '-acetoxyethoxymethyl)-5-ethyl-6-(3' ,5 '-dimethylphenoxy) -2,4-pyrimidinedione; 1- ( 2 '-hydroxyethoxymethyl )-5-ethyl-6-(3' ,5'-dimethyl- phenoxy)-2,4-pyrimidinedione; l-(2 '-acetoxyethoxymethyl)-5-isopropy1-6- ( 3 ' , 5 '-dimethyl¬ phenoxy)-2,4-pyrimidinedione; 1-(2 '-hydroxyethoxymethyl)-5-isopropyl-6-(3 ' , 5 ' -dimethyl¬ phenoxy)-2, 4-pyrimidinedione; l-(2 '-methoxyethoxymethyl)-5-isopropyl-6-( 3 ' , 5 '-dimethyl¬ phenoxy)-2,4-pyrimidinedione; l-ethoxymethyl-5-ethyl-6- ( 3 ' , 5 ' -dimethylbenzoyl ) -2 , 4- pyrimidinedione; l-ethoxymethyl-5-isopropyl-6-(3 ' ,5 ' -dimethylbenzoyl ) -2, 4- pyrimidinedione; l-ethoxymethyl-5-ethyl-6-benzoyl-2, 4-pyrimidinedione; l-ethoxymethyl-5-isopropyl-6-benzoyl-2, 4-pyrimidinedione; l-benzyloxymethyl-5-ethyl-6-(3 ' ,5 ' -dimethylbenzoyl) -2, 4- pyrimidinedione; l-benzyloxymethyl-5-isopropyl-6-(3 ' ,5 '-dimethylbenzoyl)-2,4- pyrimidinedione; l-benzyloxymethyl-5-isopropyl-6-benzoyl-2,4-pyrimidinedione; l-(2 '-hydroxyethoxymethyl)-5-isopropyl-6- ( 3 ' , 5 '-dimethyl¬ benzoyl)-2,4-pyrimidinedione; and l-(2 '-methoxyethoxymethyl)-5-isopropyl-6-(3 ' , 5 ' -dimethyl¬ benzoyl)-2,4-pyrimidinedione. 6. A process for the preparation of the compound of claim 1 which comprises silylating a compound of formula(II) to give a compound of formula(III) and coupling the compound of formula(III) with a compound of formula(IV) R'L (IV) wherein, R '1 , R 2 , R 3 , R 4 and A have the same meanings as defined in claim 1. 7. The process of claim 6 wherein the coupling reaction is conducted using the compound of formula(III) and the compound of formula(IV) in a molar ratio of 1:0.8 to 1:1.2 in a nonpolar solvent at a temperature ranging from - 20 to 100°C. 8. An antiviral composition comprising a therapeutically effective amount of the 2,4-pyrimidinedione compound or a pharmaceutically acceptable salt thereof of claim 1 as an active ingredient, and a pharmaceutically acceptable carrier and/or adjuvant.

Inventors: CHO EUI HWAN, CHUNG SUN GAN, KIM HYUN SOOK, KIM JIN CHEL, KIM JOUNG YOUNG, KWON HO SEOK, LEE ILL YOUNG, LEE JAE WEUNG, LEE SOON HWAN, SON JONG CHAN, CHO, EUI, HWAN, CHUNG, SUN, GAN, KIM, HYUN, SOOK, KIM, JIN, CHEL, KIM, JOUNG, YOUNG, KWON, HO, SEOK, LEE, ILL, YOUNG, LEE, JAE, WEUNG, LEE, SOON, HWAN, SON, JONG, CHAN
Applicants: CHO EUI HWAN, CHUNG SUN GAN, KIM HYUN SOOK, KIM JIN CHEL, KIM JOUNG YOUNG, KOREA RES INST CHEM TECH, KWON HO SEOK, LEE ILL YOUNG, LEE JAE WEUNG, LEE SOON HWAN, SAMJIN PHARM CO LTD, SON JONG CHAN, CHO, EUI, HWAN, CHUNG, SUN, GAN, KIM, HYUN, SOOK, KIM, JIN, CHEL, KIM, JOUNG, YOUNG, KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY, KWON, HO, SEOK, LEE, ILL, YOUNG, LEE, JAE, WEUNG, LEE, SOON, HWAN, SAMJIN PHARMACEUTICAL CO., LTD., SON, JONG, CHAN
Application year: 1997
Main classification: C07D239
IPC codes: A61K31, A61P31, C07D239