PROCESS FOR THE PRODUCTION OF A POROUS CROSS-LINKED POLYSACCHARIDE GEL AND ITS USE AS A GEL FILTRATION MEDIA AND IN CHROMATOGRAPHY

Abstract: A process for the production of a porous cross-linked polysaccharide gel and a gel obtainable by the following steps: a) preparing a solution or dispersion of the polysaccharide; b) adding a bifunctional cross-linking agent having one active site and one inactive site to the solution or dispersion from step a); c) reacting hydroxylgroups of the polysaccharide with the active site of the cross-linking agent; d) forming a polysaccharide gel; e) activating the inactive site of the cross-linking agent; f) reacting the activated site from step e) with hydroxylgroups of the polysaccharide gel, whereby cross-linking of the gel takes place. The cross-linked polysaccharide gel obtained can further be cross-linked by conventional methods, one or several times.

Description: PROCESS FOR THE PRODUCTION OF A POROUS CROSS-LINKED POLYSACCHARIDE GEL ANDITS USEAS A GELFILTRATION MEDIA ANDIN CHROMATOGRAPHY The present invention relates to a process for the pro¬ duction of a cross-linked polysaccharide gel with improved qualities and a gel obtainable by the process and use thereof More precisely the invention refers to a new me od of cross-linking, in which a bifunctional cross- linking agent is introduced into the polysaccharide solu¬ tion before emulsion and gel formation. Chromatographic methods are commonly used for separa¬ tion and purification of molecules such as proteins, nu¬ cleic acids, polysacchandes etc A wide variety of separa- tion media is available, both inorganic material as well as syntnetic polymers and polysacchandes. Gel matrices of polysacchandes have long been used as separation media due to their good qualities and such ma¬ trices are now standard equipment in biochemistry laborato- ries The polysacchandes are inert to biomolecules under a wide range of conditions. The polysacchandes are natural materials and as such are approved of by authorities (such as the Food and Drug Administration (FDA) n USA) for many fields of application. When using chromatographic separa- tion methods, there can be left traces of the separation medium left in the separated product When polysacchandes are used, as separation medium, this is harmless, as the matenal is not toxic. Generally, chromatographic separations are carried out in columns packed with the separation matrix m form of particulate beads. Separation media of a fast kinetics witr. rap d flow rates results in a high productivity and may be acmeved r>y a reduction of the particle size. However, small beads result in a higher back pressure due to tne narrowing of the convective flow channels netween the par¬ ticles ir. a packed bed. To be able to separate large mole¬ cules the particles should have large pores,but large pores mav resu-t in a weakened structure of the Oarticles. As the polysaccharides are soft materials the particles may easily collapse, especially at high flow rates. Thus, there is a demand on methods of manufacturing more stable polysaccha¬ ride particles. It is well known to increase the stability of polysaccharide particles by cross-linking the polymer. The cross-linking process stabilises the polysaccharide gel matrices by chemically binding the polymer chains with each other at their respective free hydroxyl groups. The cross¬ linking takes place between the hydroxyl and the functional groups of the cross-linkers. This affects the particle ri¬ gidity, but to a lesser extent or not at all the size of the pores. There are several patents and articles disclos¬ ing different cross-linking methods. Well known cross¬ linking agents are epichlorohydrin, bis-epoxides, divinyl sulphone . In EP 203 049 it was found that the rigidity of the polysaccharides was considerably improved when the cross¬ linking agent used was monofunctional but also contained an additional masked functional group that could be activated later. The cross-linking was made in two steps. First the polysaccharide was derivatized with the monofunctional group. Then, in a next step the masked group was activated and made to react with the hydroxyl groups of the polysac¬ charide. In this manner the length of the cross-linking was controlled and the desired rigidity obtained. The common characteristic for the state of the art methods is that the cross-linking is made on the polysac¬ charide polymer after the formation of the gel particles. Thus, the cross-linking is made on the ready made struc- ture . Particles of e.g. agarose are prepared by dissolving the agarose in water by heating. The hot water solution is then emulsified to form spherical particles in an organic solvent such as toluene. The particles are precipitated af¬ ter cooling. The particles are then cross-linked. By vary- ing the concentration of the agarose solution, different pore sizes can be obtained. The lower the concentration of the agarose solution the larger pores are obtained. The object of the present invention was to obtain an improved process for the production of a cross-linked poly¬ saccharide gel . A further object of the invention was to produce rigid polysaccharide gel particles with improved capability to withstand high flow rates/back pressures, but with retained separation qualities. The objects of the invention are achieved by the proc¬ ess and the polysaccharide gel as claimed in the claims. According to the invention a process for the production of a porous cross-linked polysaccharide gel is obtained, which process is characterized by the following steps: a) preparing a solution or dispersion of the polysac¬ charide, b) adding a bifunctional cross-linking agent having one active site and one inactive site to the solution or dis¬ persion from step a) , c) reacting hydroxyl groups of the polysaccharide with the active site of the cross-linking agent, d) forming a polysaccharide gel, e) activating the inactive site of the cross-linking agent , f) reacting the activated site from step e) with hy¬ droxyl groups of the polysaccharide gel, whereby cross- linking of the gel takes place. According to a further aspect of the invention a porous cross-linked polysaccharide gel is obtainable by the fol¬ lowing steps : a) preparing a solution or dispersion of the polysac- charide, b) adding a bifunctional cross-linking agent having one active site and one inactive site to the solution or dis¬ persion from step a) , c) reacting hydroxyl groups of the polysaccharide with the active site of the cross-linking agent, d) forming a polysaccharide gel, e) activating the inactive site of the cross-linking agent , f) reacting the activated site from step e) with hy¬ droxyl groups of the polysaccharide gel, whereby cross¬ linking of the gel takes place. With the present invention it was surprisingly found that gels with increased pressure/flow capacities of more than 300 % could be obtained, compared with known gels. It was possible to manufacture highly rigid gel particles also with small particle diameters (about 10 μm) . According to the new method of the invention the cross- linking agent, is introduced into the polysaccharide solu¬ tion or dispersion before the gel formation. The cross¬ linking agent is a bifunctional agent with one active site and one inactive site. When added to the polysaccharide so¬ lution or dispersion the active site of the agent is al- lowed to react with the hydroxyl groups of the polysaccha¬ ride. Thereby the cross-linking agent is chemically bound to the polymer chains before the gel formation process is started. In this manner an internal cross-linking agent is introduced into the polysaccharide. In the first step of the process a solution or disper¬ sion of the polysaccharide is formed. Solvents or dispers¬ ing agents commonly used together with polysaccharides can be used such as acetone, acetonitrile, dimethyl sulphoxide, dimethylformamide, pyridine, sec. and tert. alcohols, such as isopropanol, etc. However, according to a preferred em¬ bodiment of the invention an aqeous solution of the poly¬ saccharide is used. After the introduction of the cross-linking agent a gel is formed of the polysaccharide. If water has not been used as the solvent, the solvent or dispersing agent is then disposed of and the polysaccharide is dissolved in water. The gel is formed by emulsifying the water solution in an organic solvent such as toluene or heptane. Then, the inac¬ tive site of the cross-linking agent is activated and re- acted with hydroxyl groups of the polysaccharide, whereby the gel is cross-linked. The cross-linked gel can be further cross-linked by conventional methods as known by the state of the art. This further cross-linking can be made one or several times de¬ pending on how rigid particles that are required. The con¬ ventional cross-linking can also be made on the gel from step d) before or at the same time as activating and reac - ing of the inactive site of the cross-linking agent in steps e) and f) . In a further embodiment of the invention steps b) and c) can be repeated one or several times after steps d) or e) in order to add more cross-linking agent be¬ fore performing steps e) and f) or step f) . The bifunctional cross-linking agent used according to the invention comprises one active site and one inactive. With active site is meant all groups capable of reaction with the hydroxyl groups of the polysaccharide. Examples of such groups are halides, epoxides, ethylol groups. The in- active site is a group which does not react under the reac¬ tion conditions for the reactive site but can later on be activated to react with the hydroxyl groups of the polysac¬ charide. Groups containing double bonds such as allyl, vi¬ nyl, acryl groups are suitable. The group connecting the active and inactive site is not essential, it should how¬ ever, lack binding activity and not be too long. Preferable cross-linking agents are allylglycidyl ether and allylhal- ides, preferably allylbromide, but it is also possible to use e.g. N-methylol acrylamide, vinyl benzylchloride, cin- namoyl chloride. The reactions between the hydroxyl groups and the active site and the activated inactive site, as well as the activation of this site, is well known chemis¬ try per see. The reaction with the bifunctional cross-linking agent could be illustrated on agarose (AG) with the following re¬ action formulae: Reac tion wi th the ac tive si te of al lylglycidyl ether : NaOH AG-OH + CH 2 =CH-CH 2 -0-CH 2 -CH-CH 2 → AG-0-CH 2 .CH-CH O-CH r CH=CH 2 O OH Activation and reaction of inactive site: pH=7 AG-0-CH 2 .CH-CH 2 -0-CH 2 -CH=CH 2 +Br 2 /H 2 0→AG-0-CH 2 .C HH--CCHH 22 --00--CCHH 22 --CCHH--CCHH ?f ++HBr ΘH m OH Br NaOH AG-0-CH 2 .CH-CH 2 -0-CH 2 -CH-CH 2 →AG-0-CH 2 .CH-CH 2 -0-CH 2 -CH-CH 2 +HBr AG-0-CH 2 .CH-CH 2 -0-CH 2 -CH-CH 2 +OH-AG→AG-O-CH 2 .CH-CH 2 -0-CH 2 -CH-CH 2 -O-AG OH O OH OH The further cross-linking by conventional methods can be obtained by any of the known cross-linking agents. Suit¬ able compounds are one or several from the group of epiha- lohydrin, bis-epoxides, divinylsulphone, allylglycidyl ether and dibromopropan-1-ol. Thus, the conventional cross- linking can be made with the same cross-linker as in the internal first cross-linking step or with another cross- linker or with a mixture of cross-linkers. The process according to the invention can be used on all type of polysaccharides such as agarose, agarose de- rivatives, starch derivatives, dextran or derivatives thereof, alginate, carrageenan. However, agarose is the preferred one. The gel matrix according to the invention is preferably prepared as particles. The manufacture of the gel is made with well known methods. Agarose for example, is dissolved in water by heating the solution above the gel point for agarose. The cross-linking agent is added to the hot aque¬ ous agarose solution and the active site of the agent is allowed to react with the hydroxyl groups of the agarose. The agarose solution is then emulsified in an organic sol¬ vent such as toluene. The gel particles are precipitated by cooling. Thereafter, the inactive site of the cross-linking agent is activated and reacted with hydroxyl groups of the agarose particles whereby the gel is cross-linked. The size of the particles is determined by the stirring speed when emulsifying the agarose solution. The final re¬ quired particle size is obtained by sieving. The pore sizes are regulated e.g. by varying the polysaccharide concentra- tion. The process according to invention can be used to manufacture polysaccharide particles with conventional di¬ ameters and pore sizes. For the production of agarose par¬ ticles the concentration suitably is from 0.5 - 20 % by weight, preferably from 1 - 12 % by weight. The particle diameters can be from 1 mm - 1 μm, preferably from 500 μm - 1 μm, most preferably 200 μm - 1 μm. With the invention it is possible to produce highly rigid polysaccharide particles. The major parameter that influences the rigidity is the amount of added cross- linker, even if also the polysaccharide concentration has a significance for the rigidity and not only for the pore size as mentioned above. To obtain rigid particles the cross-linker concentration should preferably be within the range 30 - 80 μmols/g gel, most preferably 45 - 60 μmols/g gel. A concentration lower than 30 μmσls/g tends to give gels with relatively low pressure/flow capacities . A con¬ centration above 80 μmols/g can result in gels which shrink to much to be acceptable. The porous cross-linked polysaccharide gel according to the invention can be used as a gel filtration medium in which the molecules are separated according to differences in their size. They can also be used, after modification, in different types of affinity chromatography. The gel can be substituted with a lot of different groups in per see known manners. Among such groups can be mentioned: 1. Positively charged groups (primary, secondary, ter¬ tiary or quaternary amino groups) , 2. Negatively charged groups (e.g. carboxy, phosphonic acid, sulphonic acid etc.) 3. Amphoteric groups 4. Groups with specific affinity (e.g. such as between IgG-binding protein (protein A, G, L etc.) and IgG, lectin and carbohydrate, antigen/hapten and antibody, (strept) avidin and biotin, 5. Complementary nucleic acids/oligonucleotides, 6. Groups with pi-electron systems 7. Chelating groups 8. Hydrophobic groups . With these groups the matrix can then be used in affin¬ ity chromatography, ion exchange chromatography, hydropho¬ bic interaction chromatography, reversed phase chromatogra- phy, covalent chromatography etc. The invention will now be illustrated by the following examples which however are not intended to limit the inven¬ tion. With parts and percent are meant parts by weight and percent by weight if not stated explicitly. Example 1 : Preparation of agarose solution An agarose solution is prepared in a batch reactor by add¬ ing 7g agarose to 100ml distilled water under stirring for 2h at 95°C. After 2h of reaction the solution is cooled to 70°C and 1ml NaOH 45% and 1,67ml allylglycidyl ether (AGE) are added to the agarose slurry. The reaction continues for 2h under stirring at 70°C. The solution is then neutralised with 0,15ml 60% acetic acid and HCl (pH=7-8) . Emulsion media The emulsion media is made in an emulsion reactor by adding 5,3g ethyl cellulose (N-50 emulsifier) to 117ml toluene under stirring at 60°C (the dissolving of N-50 in toluene takes approximately 2h) . Transfer of the agarose solution to the emulsion reactor The agarose solution is transferred to the emulsion me¬ dia. The stirring is regulated to 130 rpm. Agarose gel par- tides are thereby formed and their size can be controlled by variation of the rotation speed of the stirrer and the addition of extra N-50. The desired maximal particle size is 150 μm. If the gel particles are too large the rotation speed can be increased up to 220 rpm and extra N-50 can be added. The maximal par¬ ticle size is controlled by taking samples approximately every 10 min., which are analysed in a microscope with a built-in size graduation. Once the 150 μm are reached, the solution is cooled down. The cooling process The solution is cooled from 60°C to <25°C in approxi¬ mately 30 min. Gel washing process The gel particles are washed under stirring with ll ethanol 99,5% which is decanted. The gel is then washed on a nutsch with 4xll ethanol 99,5% and 4x11 distilled water. Activating of inactive site of allylglycidyl ether and cross-linking of the agarose = Cross-linking no.l Bromination lOg NaAc (sodium acetate) are added to a reactor con¬ taining a solution of 100ml gel and 200ml distilled water under stirring. After 5 min. bromine-water (Br2/H2θ) is added to the solution until a dark yellow colour is ob¬ tained and maintained for over 1 min. The reaction contin¬ ues for approximately 15 min. thereafter sodium formiate is added, giving the gel a white colour. The gel is washed with 3x11 distilled water. Cross-1inking 5g a2Sθ4 are added to a reactor containing a solution of 100ml brominated gel and 100ml distilled water under stirring. After 15 min. 10ml NaOH 45% and 0,3g NaBH4 are added to the solution. The reaction continues for 3h and then the temperature of the solution is increased to 40°C and the reaction goes on for 16h. The gel is washed with distilled water until the pH=7. Further cross-linking with a conventional method = Cross-linking no. 2 45, 3g Na2Sθ4 are added to a reactor containing a solu¬ tion of 100ml AGE cross-linked gel and 33,3ml distilled wa¬ ter (75% gel slurry) under stirring. The reaction tempera¬ ture is increased to 50°C and after 2h, 1,33ml 45% NaOH and 0,4g NaBH 4 are added to the solution as well as 9,33ml NaOH 45% and 10ml epichlorhydrin (ECH) , which are added during a period of 6-8h. The reaction continues over night (ca. 16h) . The gel is washed with distilled water and 60% acetic acid is added to obtain a pH=5-6. The gel is then sieved to the desired particle size in- tervals (40-100 μm) . Example 2 : In this example particles are prepared, which are cross- linked twice by conventional methods after the cross¬ linking according to the invention. Thus, particles pre- pared according to example 1 were cross-linked with Cross¬ linking no. 2 method: 118g Na2Sθ are added under stirring to a solution of 260ml ECH cross-linked gel and 87ml distilled water. The temperature is slowly increased to 50°C and after 2 hours 3,5ml NaOH 45% and 0,35g NaBH 4 are added, while 24ml NaOH 45% and 26ml ECH are slowly pumped (6-8 hours) into the so¬ lution. The reaction is kept for 16 hours and then the gel is washed with distilled water (and 0,6ml acetic acid) un¬ til pH«5-6. The gel is then sieved and tested. Example 3 : As a comparable example agarose gel matrices allylated af¬ ter the emulsion process are prepared. A synthesis method similar to the synthesis method described in example 1 for allylation before emulsion is used. These products have the same allyl concentration as those produced with the newly developed technique. The alternative synthesis method used to produce these gels consists of the following steps: Emulsion 28g agarose are added to 400ml distilled water and heated to 95°C for 2 hours. Thereafter the solution is cooled to 70°C and transferred to a 60°C warm solution con¬ taining 470ml toluene and 35g N-50 (emulsifier) . After ap¬ proximately 45min. stirring an average particle size of 150 μm is obtained. The solution is cooled for about 30 min. to 22°C and washed with absolute ethanol (4x21) and distilled water (4x21) . Allylation: 33, 5g Na2Sθ 4 and l,8g NaBH4 are added to a solution containing 355ml agarose gel and 106,5ml distilled water. This solution is stirred for 5 min. at a temperature of 30°C and then 25ml AGE and 71ml NaOH 45% are slowly pumped (6-8 hours) to the solution. The stirring continues for 16 hours at the same temperature. The allylated gel is washed with distilled water (3x21) . Bromination: 36g sodium acetate is added to a solution of 360ml al¬ lylated gel and 720ml distilled water. After 5 min. stir¬ ring, 146ml Br2/H2θ is added to the solution and the reac¬ tion is run for 15 min. Then 0,5g sodium formate is added and the gel obtained a white colour. The gel is washed with 3x2 gel volume of distilled water. Cross-linking no. 1: 18g Na2Sθ is added to a solution of 360ml brominated gel and 360ml distilled water. The mixture is kept under stirring and after 15 min. 72ml NaOH 45% is pumped (for 30 min.) into the solution together with l,08g NaBH 4 . After 3 hours the temperature is increased to 40°C and the reaction is held for 16 hours. The gel is then washed with distilled water until pH is about 7. Cross-linking no. 2: 156, 4g a2≤θ4 is added to a solution containing 345ml cross-linked gel and 115ml distilled water. The solution is kept under stirring and slowly heated to 50°C. After 2 hours 4,6ml NaOH 45% and 0,46g NaBH 4 are added to the mix¬ ture while 32,2ml NaOH 45% and 34,5ml ECH are pumped for 6- 8 hours to the solution. The reaction continues for 16 hours and then the gel is washed with distilled water (and 0,6ml acetic acid) until pH is about 5-6. The gel is sieved and tested. Example 4 : Extra allylation of an allylated agarose gel matrix after cross-linking no. 1 During the allylation process of the agarose solution, the bifunctional AGE molecule binds to the agarose polymer chains with its active site leaving its inactive site free. The free site is first brominated and during cross-linking no. 1 it is epoxidized with NaOH, enabling the AGE molecule to bind to a second agarose polymer chain. The alternative synthesis method here aims at coupling more AGE to the polymer chains by repeating the allylation process one more time before starting the first cross-linking as it is ex¬ plained experimentally in the following steps: Bromination: 35g sodium acetate is added to a solution of 350ml al¬ lylated gel, prepared according to example 1, and 700ml distilled water. The mixture is kept under stirring and af¬ ter 5 min. 160ml Br 2 /H 2 0 are added. The reaction continues for 15 min. and then 0, 5g sodium formate is added. The gel colour changes to white and the gel is washed with dis¬ tilled water (3xll) . Allylation: 17, 5g Na2Sθ4, 0, 5g NaBH4 and 35ml NaOH 45% are added to a solution of 350ml brominated gel and 175ml H2O. The mix¬ ture is held under stirring 40°C and after 1 hour 20ml AGE are added. The reaction continues for 16 hours after which the gel is washed with distilled water until pH is about 7. Bromination: 38, 5g sodium acetate are added to a stirred solution containing 385ml gel allylated twice and 770ml distilled water. After 5 min. 85ml Br2/H2θ are added and the reaction is kept for 15 min. and then 0, 5g sodium formate are added giving the gel a white colour. The gel is then washed with distilled water (3x11) . Cross-linking no. 1: 19,25g Na2S0 4 are added to a stirred mixture containing 385ml of the above mentioned brominated gel and 385ml dis¬ tilled water. After 15 min. 38,5ml NaOH 45% is pumped to the solution (pumping period = 30 min.) along with l,16g NaBH^ . The reaction continues for 45 min. before the tem¬ perature is increased to 40°C. The solution is kept under stirring for 16 hours. The gel is then washed with dis¬ tilled water until pH is about 7. Cross-linking no. 2: 174, 4g Na2S0 4 are added to a stirred solution of 385ml cross-linked gel and 128ml distilled water. The mixture is slowly heated to 50°C and after 2 hours 5,13ml NaOH 45% and 0,51g NaBH 4 are added to the solution while 36ml NaOH 45% and 38,5ml ECH are slowly pumped (6-8 hours) into the reac¬ tor. The reaction continues for 16 hours, and then the gel is washed with distilled water (and 0,6ml acetic acid) un- til pH=5-6. The gel is sieved and tested. Example 5 : Second cross-linking before the first cross-linking In this synthesis the ECH cross-linking is performed before cross-linking no. 1. The experimental procedure is explained in the following steps. Cross-linking no. 2: An allylated gel is produced according to the method described in example 1. 158, 8g Na2Sθ 4 are added to a solu- tion containing 350ml of this allylated gel and 117ml dis¬ tilled water. The mixture is stirred and slowly heated to 50°C and after 2 hours 4,67ml NaOH 45% and 0,67g NaBH4 are added to the solution while 32,7ml NaOH 45% and 35ml ECH are slowly pumped (6-8 hours) into the reactor. The reac- tion is held for 16 hours and then the gel is washed with distilled water until pH is about 5-6. Bromination: 20, 5g sodium acetate are added to a solution prepared from 205ml ECH-cross-linked gel and 410ml distilled water. The mixture is stirred and after 5 min. 70ml Br2/H2θ are added and after 15 min. reaction time 0.5g sodium formate are added and the gel becomes white. The gel is then washed with distilled water (3xll) . Cross-linking no. 1: 10,25g Na2Sθ4 are added to a solution containing 205ml brominated gel and 205ml distilled water. The mixture is stirred for 15 min. and then 20,5ml NaOH 45% and 0,61g NaBH4 are added. After 3 hours reaction time the tempera¬ ture is increased to 40°C and the solution is kept under stirring for another 16 hours. The gel is then washed with distilled water until pH is about 5-6. The gel is sieved and tested. The gels prepared in the examples were analysed with respect to allyl concentration, the pressure/flow capaci- ties, Ka v values (Ka v is an accepted definition of the relative pore size) and particle size distribution accord¬ ingly: Allyl concentration analysis The allyl concentration was analysed on a Mettler DL40GP Memo Titrator with 0.1 M AgN0 3 according to standard methods . The pressure/flow capacities analysis Instrument: XK-16/40 column HR 10/30 column FPLCdirector™ system control unit (max. pres¬ sure/flow output:26-30 bar or 200-210 ml/min) : a Pharmacia Biotech Controller CC-501 Plus and two Pharmacia Biotech Pump P-6000 and PC-unit with built-in interface. Me thod : The testing of pressure/flow resistance of a gel matrix depends on the gel average bead size and the column packing process. The gel beads produced have two av ¬ erage size intervals a) 8-12 μm b) 40-100 μm. Packing : a) The column packing process for gel beads with aver¬ age size 8-12 μm is made in a HR 10/30 column with a series of pressure/time variations in the same manner as for Kav tests (see next paragraph) . b) The column packing process for gel beads with aver¬ age size 40-100 μm is a free sedimentation process made in a XK 16/40 column with a bed height of 31 ± 1 cm. Pressure/flow test: a) Tests on the HR-10/30 column are made by increasing the flow 0,1 ml/min every 5 min and reading the back pres¬ sure variation every 5 min. b) Tests on the XK-16/40 column are made by increasing the flow 1 or 5 ml/min every 5 min and reading the back pressure variation every 5 min. The Kav-values determination analysis Instrument: Pharmacia UVM-detector Pharmacia Biotech FPLCdirector™ system control 2 channel recorder (plotting unit) Method : The determination of the Kav-values of the gel matrix, results in an estimation of the pore size of the gel beads. The determination is made on the final product (the cross-linked gel) . It is performed by graphic inter¬ pretation of the retardation time of several proteins, which have been injected into a column containing gel ma¬ trix. Packing: The final product is packed in a HR 10/30 column under a pressure of 17 bar. The packing solvent used is a solu¬ tion with the following composition: 60 g HAc + 1 g Tween™ 20 per 1000 ml To pack the column 30 g gel is dispersed in 30 g pack- ing solvent. The gel matrix is first packed under a pressure of 6 bars for 50 min then under 17 bars under 10 min. Protein injection and Kav determination: The Kav-values are determined for four proteins: • Thyroglobulin (MW = 669000 g/mol) • Ferritin (MW = 440000 g/mol) • BSA (MW = 67000 g/mol) • R-nase (MW = 13700 g/mol) These proteins are injected into the column one or two at the time (to prevent overlaps to occur) . The eluent media used during this procedure is a buffer solution with pH = 7,2 and the following composition: • 50 mM Sodium dihydrophosphate (NaH P0 4 x 2H 0) • 150 mM Sodium chloride (NaCl) • 0,02 % Sodium azide (NaN 3 ) To determine the Kav-values it is necessary to know the volume occupied by the void (volume around the agarose beads) which is done by injecting blue dextran into the column. The obtained plots are interpreted and the data is ana¬ lysed with a PC which calculates the desired Kav-values. The particle size distribution analysis: The mean particle size distribution (d50v)was performed with a Coulter Multisizer. The results from examples 1 - 5 are compared with a standard agarose gel (Sepharose®6FF) and presented in the following tables : Table la: Gel Allyl d50v Pressure/Flow test no. cone. Max Max Pressure In¬ flow Pres. crease [μmol/g [μm] [cm/h] [bar] >1 bar* >1,5 gel] bar* Seph.δFF - 91.1 1050 6.00 2.25 3.50 Example 1 53 74.9 3450 17.25 12.00 14.25 Example 2 53 74.9 4500 17.25 12.90 14.10 Example 3 45 71.4 1500 13.00 4.00 5.25 Example 4 46 77.2 >6000** 12.90 >12.90 >12.90 Example 5 46 83.7 5250 18.80 13.90 15.10 * Pressure for which the pressure increase is over 1 or 1.5 bar when the flow is increased by 150 cm/h every 5 min. ** >6000 cm/h indicates the maximal flow the instrument is capable of delivering. The maximal flow capacity of the gel lies above this value. Table lb: Gel Allyl Kav no. cone. Thyro Ferritin BSA R-nase [μmol/g gel] Seph.δFF - 0.37 0.48 0.64 0.81 Example 1 53 0.34 0.48 0.64 0.82 Example 2 53 0.33 0.48 0.64 0.80 Example 3 45 0.28 0.44 0.61 0.80 Example 4 46 0.28 0.43 0.56 0.70 Example 5 46 0.27 0.42 0.58 0.74 Example 6 : In this example the method according to the invention was used to prepare highly rigid agarose gel beads with 8.1 w/v % agarose. The process according to example 2 was re¬ peated but when preparing the agarose solution 8.1 g aga- rose per 100 ml water was used. The gel was sieved and two fractions with two different particle sizes were obtained, Example 6a and 6b. A further particle size, Example 6c was produced according the same manner. The gel beads were tested in the same way as mentioned above. The prepared particles were compared with conventional particles with an agarose content of 8.1 w/v % (Superose®6 from Pharmacia) . The test results are put together in tables 2a and 2b. Table 2a : Gel Allyl d50v Pressure/Flow test no. cone . Max flow Max Pres [μmol/g [μm] [ml/min] [bar] gel] Superose 6 - 13.2 0,9 15 Superose 6 - 14.6 1,2 17 Example 6a 59 9.2 >1.7 >26* Example 6b 59 12.6 >2.5 >26* Example 6c 57 11.2 >2.5 >26* * 26 bar corresponds to the maximal pressure the instrument is capable of delivering. The maximal pressure and flow values obtained above are expected to be higher. Table 2b: Gel Allyl d50v Kav no. cone. Thyro Ferritin BSA R-nase [μmol/g [μm] gel] Superose 6 - 13.2 0.29 0.40 0.54 0.68 Superose 6 - 14.6 0.36 - 0.60 - Example 6a 59 9.2 0.24 0.37 0.50 0.68 Example 6b 59 12.6 0.26 0.39 0.52 0.68 Example 6c 57 11.2 0.21 0.34 0.47 0.63 In the following examples gels with different agarose content were produced: Example 7 : The method according to example 1 was repeated but with different agarose contents. The result is disclosed in ta¬ ble 3a and 3b below. Example 7a with 7 % agarose is identi- cal with example 1. Example 8 : In this example different agarose contents were used and the method according to example 5 was used. The result is disclosed in the tables below. Table 3a: Gel Agarose d50v Pressure/Flow test [w/v %] Max Max Pressure In¬ flow Pres . crease [μm] [cm/h] [bar] >1 bar* >1,5 bar* Seph.4FF 4 98.6 480 1.8 - - Seph.6FF 6 91.1 1050 6.0 2.25 3.5 Example 7a 7 74.9 3450 17.25 12.0 14.25 Example 7b 4 84.0 2250 9.00 5.45 6.60 Example 8a 7 78.4 >6000** 19.90 >19.90 >19.90 Example 8b 5 61.7 3150 16.80 9.55 12.30 Example 8c 4 - 2700 9.50 6.25 7.40 Example 8d 3 87.5 1800 4.5 >4.5 >4.5 * Pressure for which the pressure increase is over 1 or 1.5 bar when the flow is increased by 150 cm/h every 5 min. ** >6000 cm/h indicates the maximal flow the instrument is capable of delivering. The maximal flow capacity of the gel lies above this value. Table 3b: Gel Agarose Kav [w/v %] Thyro Ferritin BSA R-nase Seph.4FF 4 0.57 0.66 0.76 0.87 Seph.4FF 6 0.37 0.48 0.64 0.81 Example 7a 7 0.34 0.48 0.64 0.82 Example 7b 4 0.53 0.63 0.76 0.87 Example 8a 7 0.26 0.43 0.57 0.73 Example 8b 5 0.36 0.51 0.63 0.77 Example 8c 4 0.56 0.64 0.78 0.88 Example 8d 3 0.67 0.77 0.84 0.93 Conclusion: From the tables it can be seen that the use of the new cross-linking method according to the invention results in gels capable of withstanding more than three times higher flow than conventional gel particles or particles prepared according to known methods (Example 3) , though the gels have similar K av values. The excellent behaviour of the gels of the invention can also be illustrated as in figures 1 - 3. In the fig¬ ures : Figure 1 is a plot of the flow against the back pres¬ sure of examples 1 - 5 and comparable compound. Figure 2 is a plot of the flow against the back of ex- amples 6a, 6b, 6c and comparable compound. Figure 3a is a plot of the Kav values against the maxi¬ mal flow for examples 7a, 7b, 8a, 8b, 8c, 8d and comparable compounds . Figure 3b is a plot of the flow against the back pres- sure for the same examples as in figure 3a. From the figures it is evident that for the state of the art particles the back pressure raises quickly when the flow increases above a moderate value, which indicates col¬ lapse of the particles if the flow is increased too much. However, the pressure/flow plots for the gels according to the invention show a much lower inclination, indicating that the back pressure only raises slowly when the flow is increased. In figure 3a the Kav values for the gel matrices ac- cording to examples 7a,b and 8a,b,c,d has been plotted against the maximal flow. It is readily seen from the dia¬ gram that the maximal tolerated flow is increased by 300 % for matrices produced according to example 7 or example 8, idenpendently from the agarose w/v percentage. However, the agarose w/v percentage has an important impact on the gel beads pore size, which is expressed by the Kav values, as they increase with the reducing amount of agarose in the gel beads .

Claims: 1. A process for the production of a porous cross- linked polysaccharide gel, characterized by the following steps : a) preparing a solution or dispersion of the polysaccharide, b) adding a bifunctional cross-linking agent having one active site and one inactive site to the solution or dispersion from step a) , c) reacting hydroxylgroups of the polysaccharide with the active site of the cross-linking agent, d) forming a polysaccharide gel, e) activating the inactive site of the cross-linking agent, f) reacting the activated site from step e) with hydroxylgroups of the polysaccharide gel, whereby cross- linking of the gel takes place. 2. A process according to claim 1, characterized in that the cross-linked polysaccharide gel obtained is further cross-linked by conventional methods, one or several times. 3. A process according to claim 1 or 2, characterized in that the gel from step d) is cross-linked by conventional methods before performing steps e) and f) . 4. A process according to claims 1 or 2, characterized in that the gel from step d) is cross-linked by conventional methods at the same time as performing steps e) and f) . 5. A process according to claim 1 or 2, characterized in that steps b) and c) are repeated one or several times after steps d) or e) before performing steps e) and f) or step f) . 6. A process according to any of the preceding claims, characterized in that an aqueous solution is prepared of the polysaccharide in step a) . 7. A process according to claim 6, characterized m that the gel is formed by emulsifying the aqueous solution from step c) to particles, in an organic solvent. 8. A process according to any of the preceding claims, characterized in that the bifunctional cross-linking agent is allylglycidyl ether or allylbromide. 9. A process according to claim 2-5, characterized in that the further cross-linking by conventional methods is obtained by one or several compounds from any of epihalohydrin, bis-epoxides, divinylsulphon, allylglycidyl ether and dibromopropan-1-ol . 10. A process according to any of the preceding claims, characterized in that polysaccharide is agarose. 11. A porous, cross-linked polysaccharide gel obtainable by the following steps: a) preparing a solution or dispersion of the polysaccharide, b) adding a bifunctional cross-linking agent having one active site and one inactive site to the solution or dispersion from step a) , c) reacting hydroxylgroups of the polysaccharide with the active site of the cross-linking agent, d) forming a polysaccharide gel, e) activating the inactive site of the cross-linking agent, f) reacting the activated site from step e) with hydroxylgroups of the polysaccharide gel, whereby cross- linking of the gel takes place. 12. A polysaccharide gel according to claim 11, characterized in that the cross-linked polysaccharide gel obtained is further cross-linked by conventional methods, one or several times. 13. A polysaccharide gel according to claim 11 or 12, characterized in that the gel from step d) is cross-linked by conventional methods before performing steps e) and f) . 14. A polysaccharide gel according to claim 11 or 12, characterized in that the gel from step d) is cross-linked by conventional methods at the same time as performing steps e) and f) . 15. A polysaccharide gel according to claim 11 or 12, characterized in that steps b)and c) are repeated one or several times after steps d) or e) before performing steps e) and f) or step f) . 16. A polysaccharide gel according to any of the preceding claims, characterized m that an aqueous solution is prepared of the polysaccharide in step a) . 17. A polysaccharide gel according to claim 16, characterized in that the gel s formed by emulsifying the aqueous solution from step c) to particles, in an organic solvent . 18. A polysaccharide gel according to any of the preceding claims, characterized in that the bifunctional cross-linking agent is allylglycidyl ether or allylhalide. 19. A polysacchande gel according to claim 12, characterized in that the cross-linked polysaccharide gel obtained is further cross-linked, by one or several compounds from any of epihalohydrm, bis-epoxides, divinylsulphon, allylglycidyl ether and dιbromopropan-1-ol . 20. A polysaccharide gel according to any of the preceding claims, characterized in that polysaccharide is agarose . 21. Use of a porous, cross-linked polysaccharide gel according to any of the preceding claims as a gel filtration medium, m affinity chromatography, ion exchange chromatography, hydrophobic interaction chromatography, reversed phase chromatography chelate chromatography, covalent chromatography.

Inventors: BERG HANS, BERG, HANS
Applicants: BERG HANS, PHARMACIA BIOTECH AB, BERG, HANS
Application year: 1997
Main classification: C08B37
IPC codes: B01D15, B01J20, C08B37