Measurement method and kit for hemoglobin A1c

Abstract: A sample containing hemoglobin A1c (HbA1c) is simultaneously brought in contact with a solid phase and anti-HbA1c antibody, the anti-HbA1c antibody bound to the adsorbed HbA1c on the solid phase and the anti-HbA1c antibody in solution are separated, and the anti-HbA1c antibody bound to the adsorbed HbA1c on the solid phase is detected. The present invention enables HbA1c % to be determined by a one-step immmunoassay, and the time required for measurement can be shortened in comparison with conventional immunoassay methods. Consequently, since the time in contact with the pretreatment solution also present in the reaction solution during the immune reaction is shortened, formation of a precipitate in the sample as well as inactivation of the enzyme of the enzyme-labeled anti-HbA1c antibody can be reduced.

Description: The present invention relates to a one-step immunoassay method for measuring the percentage of hemoglobin A1c in a sample. Hemoglobin Alc (HbA1c) is a glycosylated hemoglobin formed by binding between the amino group of the N-terminal valine of the β-chain of hemoglobin and the glucose aldehyde group resulting first in forming a Schiff's base and further forming a stable ketoamine by the Amadori Rearrangement. Since percentage of HbA1c reflects mean blood sugar content prior to 1 to 2 months, it is well accepted for blood sugar level management. So far, an instrument performing measurement by high performance liquid chromatography (HPLC) using ion exchange chromatography, namely a glycohemoglobin analyzer, has been primarily used for the measurement of HbA1c % in the blood. Although this method gives excellent reproducibility, it has also been pointed out that several disadvantages exist, including specialized HPLC analyzer system requirement, limitations of processing capacity, and inaccurate results with other forms of hemoglobins. In addition, it requires highly technical maintenances. In recent years, HbA1c measurement techniques have been developed other than using the HPLC method to solve the above-mentioned problems, and such reagants have been marketed. In particular, immunoassay has been applied for the measurement of HbA1c % due to its high specificity. Sandwich assay, for example, using two antibodies has been considered for use as an immunoassay for HbA1c. On the other hand, an adsorption-immunoassay technique wherein hemoglobins including HbA1c in a sample are physically adsorbed to a solid phase and the adsorbed HbA1c is detected with an antibody having high specificity for HbA1c is well known. In this method wherein hemoglobins are directly adsorbed to a solid phase, adsorbed HbA1c is considered to be in a proportion equal to the proportion in hemoglobins when excess hemoglobins are applied to the limited surface of the solid phase. Since HbA1c is expressed in the clinical field as percentage (%) relative to the total amount of hemoglobins, the percentage of HbA1c in the blood can be determined without measuring the absolute amount of HbA1c or of the total hemoglobins, when the percentage of HbA1c of the adsorbed hemoglobins on a solid phase is in a proportion equal to that in the blood. In this method it is required for hemoglobins to be adequately adsorbed on the solid phase. Sequential steps are, therefore, carried out in this method. Namely, hemoglobins are first adsorbed on the surface of a solid phase such as latex particles or polystyrene beads, the resulting solid phase is then washed or separated to remove unadsorbed materials as necessary, and the HbA1c adsorbed on the solid phase is specifically detected by anti-HbA1c antibody that specifically binds HbA1c. Enzyme immunoassay (EIA) reagents using this method are also commercially available. In this two-step assay using a commercially available EIA kit, since washing procedure is required between the first adsorption step and the second immunoreaction step, the procedure is more complex and requires longer processing time as compared with simultaneous or one-step immunoassay. In addition, there is also a possibility of the hemoglobin adsorbed onto the solid phase being released during washing step. Moreover, in these types of kits, since the glycosylated N-terminal residue of the hemoglobin β-chain is used for the reaction with a specific antibody to HbA1c, there are many cases to require strong denaturing conditions to expose the glycosylated N-terminal residues during or prior to adsorption to the solid phase. However, since there is a high possibility that the enzyme antibody conjugate may be inactivated under these conditions, a simultaneous adsorption-immune reaction step may not be applied due to possible inactivation of the enzyme and/or the antibody of the enzyme labeled anti-HbA1c antibody. Due to the strong denaturing conditions used in such commercially available kits, denatured blood sample is required to be used immediately after its preparation, because if time elapses variation occurs in the measured values due to the formation of precipitates and so forth, thus preventing the obtaining of correct values. Consequently, this method is not considered to be suitable for large number of sample processing. On the other hand, an immunoassay has also been applied to latex agglutination instead of EIA. A hemolyzed specimen is added to an unsensitized latex suspension to adsorb the hemoglobins on the surface of latex first, then the mouse monoclonal antibody specific to HbA1c is added followed by the addition of anti-mouse IgG polyclonal antibody to result in agglutination whereby the degree of agglutination reflects HbAlc % in a sample. Although this method does not require a washing step, since a large amount of latex is required to adsorb hemoglobins, there is a possibility of the occurrence of non-specific agglutination of latex resulting in the appearance of abnormally high values or the risk of it being difficult to detect increased agglutination caused by antibody-antigen reaction of HbA1c. Diabetes and other adult related diseases are steadily increasing due to the increase of the elderly population. Accompanying this trend, since diagnostic parameters such as HbA1c % which is widely recognized as an indicator of blood sugar status is the object of diagnosis when examinations are required to be performed on elderly persons, it is necessary to improve specimen processing capacity. There is therefore a tremendous need for the development of diagnostic reagents that enable processing to be performed easily, accurately and in large volume. The currently available HPLC method has a low specimen processing capacity and specialized instrumentation requirement with complexity in maintenance. On the other hand, the currently available EIA and latex agglutination methods require complex procedures and give inaccurate results due to several reasons mentioned above. As the result of conducting studies on the method enabling measurement of HbA1c % in a sample easily and accurately on the basis of immunoassay with high specificity, the inventors of the present invention constructed a method that allows measurement of HbA1c % in a sample using a one-step procedure in which adsorption of hemoglobins on the solid phase and reacting of antigen HbA1c and anti-HbA1c antibody are performed simultaneously, thereby leading to completion of the present invention. Namely, the present invention discloses a method for measuring HbA1c % comprising the steps of bringing a sample containing HbA1c into simultaneous contact with a solid phase and a labeled anti-hemoglobin A1c antibody resulting in the formation of adsorbed HbA1c bound to the labeled antibody, separating the labeled anti-hemoglobin A1c antibody bound to hemoglobin A1c which is bound to the solid phase from unbound labeled anti-hemoglobin A1c antibody and detecting the amount of the label of bound anti-hemoglobin A1c antibody. Fig. 1 is a standard curve for the measurement of HbA1c % using the one-step 40-minute incubation reaction employed in Example 1. Fig. 2 is a correlation diagram between the one-step 40-minute immunoassay method employed in Example 2 and a conventional HPLC method. Fig. 3 is a standard curve for the measurement of HbA1c % using one-step 10-minute incubation reaction employed in Example 4. Fig. 4 is a correlation diagram between the one-step immunoassay method employed in Example 4 in which the incubation time was shortened to 10 minutes and a conventional HPLC method. In the present invention, it is necessary to hemolyze blood samples prior to measuring HbA1c % in the blood. There is no particular limitation on the method for hemolyzing the blood, and various methods can be used, examples of which include the use of a hypotonic liquid such as water or a surfactant. After hemolysis, pretreatment of the sample is performed. HbA1c is denatured and unstable HbA1c can be removed by this pretreatment. There is no particular limitation required for the pretreatment step, and various methods can be employed. For example, KSCN or other chemicals can be applied. However, in order to minimize detrimental effects on the immunoassay method in the next step, namely to reduce precipitate formation of the sample caused by the pretreatment solution still present in the reaction mixture, and to reduce inactivation of the enzyme and/or the antibody in the case of using enzyme-labeled antibody, it is preferable to use mild conditions and less amount in pretreatment. Thus, it is preferable that pretreatment be performed under, for example, mildly acidic conditions. Mildly acidic conditions refer to a pH of preferably 3.0 to 6.9 and more preferably 4.0 to 6.0. Examples of buffers used for pretreatment under these conditions include aliphatic carboxylate buffers or borate buffers. Examples of aliphatic carboxylate buffers include monocarboxylate and dicarboxylate buffers, and more specifically, succinate, malonate, fumarate, maleate, glutarate or acetate buffers. Succinate, glutarate or acetate buffers are preferable, while succinate buffer is particularly preferable. Pretreatment under mildly acidic conditions enables HbA1c to be denatured while preserving the α2β2 structure of hemoglobin and unstable HbA1c to be removed. Furthermore, hemolysis and pretreatment may be performed sequentially or simultaneously. Thus, once the sample is hemolyzed and pretreated, a solid phase and a labeled anti-HbA1c antibody are simultaneously brought in contact. There is no particular limitation for the solid phase used in this method capable of adsorbing hemoglobins, and examples of the solid phase include tubes, beads, plates, microparticles or filter paper made of materials such as glass, polystyrene, latex and paper. Regardless of the solid phase used, a larger surface is preferable since it results in an increased amount of adsorbed hemoglobins and improved measurement sensitivity. On the other hand, there is also no particular limitation on the anti-HbA1c antibody used in the reaction provided it specifically recognizes HbA1c. Monoclonal or polyclonal antibody as well as their fragments may be used. The anti-HbA1c antibody is labeled to facilitate detection following the immune reaction. There is no particular limitation on this label provided it is normally used in detection of immune reactions, examples of which include enzymes, radioisotopes and fluorescent materials, with enzyme labeling being particularly preferable. More specifically, examples of enzymes include alkaline phosphatase, peroxidase and β-D-galactosidase, examples of radioisotopes include 125 I, and examples of fluorescent materials include fluorescein. Although not included by the present invention, another antibody (2nd antibody) that recognizes anti-HbA1c antibody may be labeled, and reacted and used for detection. The same labels are used in this case as those described above. Furthermore, it is preferable that an adequate amount of anti-HbA1c antibody be added for binding with the HbA1c adsorbed on the solid phase. The analyte HbA1c in a sample in the present invention is reacted simultaneously by bringing the sample into contact with a solid phase and an anti-HbA1c antibody. The adsorption of the HbA1c onto the solid phase and the reaction of the HbA1c with anti-HbA1c antibody is characterized by being performed in a single incubation step. Examples of methods for accomplishing this include a method wherein solid phase and anti-HbA1c antibody are simultaneously added to the sample, and a method wherein solid phase and anti-HbA1c antibody are mixed in advance followed by the addition of sample. There is no particular limitation on the conditions for the adsorption of the HbA1c in the sample to the solid phase and the reaction with anti-HbA1c antibody, and the conditions for ordinary immunoassay reactions can be employed. However, the reaction time should be as short as possible provided it does not hinder detection. The reaction time is normally 5 to 60 minutes, preferably 5 to 50 minutes and more preferably 5 to 15 minutes. Not only is this done to improve measurement efficiency, but also to reduce the formation of precipitates in the sample caused by the pretreatment solution still present in the reaction mixture. In addition, in the case of using an enzyme-labeled anti-HbA1c antibody, the short time incubation is also necessary to reduce inactivation of enzyme and/or antibody by the pretreatment solution. After adsorption of the HbA1c on the solid phase and reaction with anti-HbA1c antibody, the anti-HbA1c antibody bound to the HbA1c which has been adsorbed on the solid phase and unbound materials are separated and the resulted solid phase is washed if necessary. Next, anti-HbA1c antibody bound to the HbA1c adsorbed on the solid phase is detected to determine the HbA1c % in the sample. At this time, detection is performed using the label on the anti-HbA1c antibody. Any method suitable for detecting the label can be used for the detection thereof. The present invention enables HbA1c % to be measured in a one-step immunoassay. As a result, the time required for measurement can be reduced in comparison with the measurement of HbA1c in two steps as in conventional immunoassay methods. Consequently, the amount of time in contact with the pretreatment solution still present in the reaction mixture is shortened to prevent the formation of precipitates in the sample. In addition, in the case that the enyzme-labeled anti-HbA1c antibody is used in a one-step immunoassay, inactivation of the enzyme and/or antibody caused by the presence of the pretreatment solution can also be reduced when a shorter incubation time is applied. Moreover, since only one step is required for separation of bound anti-HbA1c antibody and unbound materials in solution, there is less risk of hemoglobin being released from the solid phase. Moreover, the present invention enables the most recent HbA1c % measurement values to be available before an interview with a patient by drawing blood from the patient and measuring HbA1c %. In addition, even if blood is drawn during the interview, results can be obtained without having the patient wait for so long due to the shorter incubation time of the present method. If the present method is used in combination with a commercially available automated immunoassay system, results can be obtained completely automatically with the exception of the initial hemolysis and pretreatment. In addition, since there is no need for a washing procedure during the course of the immune reaction, measurement time can be shortened thereby enabling a dramatic improvement in diagnostic usefulness. Moreover, since measurement is possible with a commercially available fully automated immunoassay system, examination of other diabetes markers, such as insulin and C-peptide, can also be performed at the same time, thereby enabling a larger amount of information to be easily obtained all at once. The examples illustrate the invention. Example 1. Establishment of HbA1c Measurement Method and Preparation of a Standard Curve Alkaline phosphatase-labeled anti-HbA1c antibody was prepared as described below. Namely, after bonding 2 mg of anti-HbA1c mouse monoclonal antibody (DAKO) with 2 mg of commercially available alkaline phosphatase derived from bovine small intestine (Biozyme) by S-S bonding, the resulting product was purified (see Ishikawa, E. ed., Enzyme Immunoassay Methods 3rd. Edition, Igaku Shoin, p. 117) by gel filtration (column: G3000SW, Tosoh; elution: 50 mM phosphate buffer (pH 7) containing 150 mM NaCl). The prepared alkaline phosphatase-labeled anti-HbA1c antibody was added to 0.1 M Tris buffer (pH 8.0) containing 5% bovine serum albumin, 10 mM MgCl 2 , 0.1 mM ZnCl 2 and 0.1% NaN 3 to a concentration of 1.5 mA (1 mA is the concentration at which absorbance at 280 nm is 0.001). 100 µl of this reaction solution was poured into a cup followed by the addition of 12 polystyrene beads (diameter: 1.4 to 1.6 mm). The calibrators were prepared by separating the red blood cells from six patients with different HbA1c % concentrations, washing the blood cells, hemolyzing them by using purified water and finally freeze-drying them. After reconstituting with purified water, the HbA1c % concentration of these calibrators was determined using a commercially available glycohemoglobin analyzer (HLC-723GHbIII, Tosoh). Reconstituted calibrator (20 µl) was added to 580 µl of 0.1 M succinate buffer (pH 5.0) containing 0.1% Triton X-100 and 0.1% NaN 3 followed by stirring and allowing to stand for 30 minutes at room temperature to prepare the sample solution for the immunoassay. The sample solution (10 µl) prepared above was added to the cup containing the above-mentioned labeled antibody and the beads, then the immunoassay was performed in a single step using a commercially available, fully automated immunoanalyzer (AIA-1200, Tosoh). Namely, after incubation for 40 minutes at 37°C, the beads, to which the antigen-antibody complex comprising the enzyme-labeled anti-HbA1c antibody and the antigen HbA1c was adsorbed, were washed followed by the addition of 220 µl of substrate solution (pH 10) containing 0.26 mg/ml of 4-methylumbelliferyl phosphate (4-MUP). The rate measurement of 4-methylumbelliferone (4-MU) formation was then performed at an excitation wavelength of 362 nm and an emission wavelength of 447 nm. Note that alkaline phosphatase converts 4-MUP to a fluorogenic substance, 4-MU. The results are shown in Fig. 1. The HbA1c % concentration of the calibrators (as determined with a glycohemoglobin analyzer) are plotted on the horizontal axis, while the rate of 4-MU formation converted by labeled alkaline phosphatase is plotted on the vertical axis. The rate of 4-MU formation increased dependent on the concentration of HbA1c (%), thereby making it possible to prepare a standard curve. Example 2. Correlation with Conventional Method using HPLC After adding 20 µl of whole blood samples, obtained from normal adults and diabetes patients using EDTA as anticoagulant, to 580 µl of 0.1 M succinate buffer (pH 5.0) containing 0.1% Triton X-100 and 0.1% NaN 3 and stirring, the mixtures were allowed to stand at room temperature for 30 minutes to complete hemolysis and pretreatment of the samples. The solutions thus obtained were used as the sample solutions for the immunoassay. The HbA1c concentration in a whole blood sample of a patient was determined in advance with a commercially available glycohemoglobin analyzer (HLC-723GHbIII, Tosoh) using HPLC technique. One-step immunoassay measurement was performed on the AIA-1200 according to the method described in Example 1 to determine HbA1c % concentration. The results are shown in Fig. 2. Fig. 2 shows the measurement results for 50 diabetic samples. HbA1c concentrations (%) as determined using the HPLC method are plotted on the horizontal axis, while concentrations determined using the method of the present invention are plotted on the vertical axis. Good correlation was observed between the two methods, yielding a correlation equation of y = 1.63 + 0.69x and a correlation coefficient of r = 0.907, thus indicating the HbA1c % consistent trend between the method of the present invention and the conventional HPLC method. Example 3. Intra-Assay and Inter-Assay Reproducibility The intra-assay reproducibility of the one-step immunoassay method described above was evaluated using patient samples of four different concentrations of HbA1c. The results are shown in Table 1. HbA1c as determined by HPLC (%) 4.50 6.10 8.20 10.00 Measured values (%) 3.81 5.66 7.01 7.44 3.86 5.45 6.68 7.98 4.02 5.21 6.58 7.82 3.85 5.38 6.8 7.47 3.86 5.2 6.86 7.7 4.1 5.3 6.8 7.67 3.94 5.27 6.94 7.98 3.8 5.04 6.42 8.21 4.15 4.83 6.98 8.33 3.71 5.35 7.96 Mean 3.91 5.27 6.79 7.86 Standard deviation 0.14 0.23 0.20 0.29 Coefficient of variation CV (%) 3.56 4.29 2.88 3.71 Intra-assay reproducibility was good, within a range of 3.5 to 4.3%, for all concentration ranges. In addition, when inter-assay reproducibility was studied using two concentrations of samples, favorable results were obtained of 4% or less as shown in Table 2. HbA1c as determined by HPLC (%) 4.8 10.3 Measured values (%) 4.88 8.28 5.13 7.82 5.00 8.44 4.86 8.51 4.95 8.33 Mean 4.96 8.28 Standard deviation 0.11 0.27 Coefficient of variation CV (%) 2.18 3.27 Example 4. Construction of a 10-Minute Assay System Although the one-step immunoassay method of Examples 1 through 3 needs an incubation time of 40 minutes, a one-step immunoassay method was also developed to decrease the incubation time to 10 minutes. Namely, an immunoassay was performed using the alkaline phosphatase-labeled anti-HbA1c antibody solution of Example 1 increased to a concentration to 5 mA. The standard curve for this method is shown in Fig. 3. The concentration of the calibrators (as determined with a glycohemoglobin analyzer) are plotted on the horizontal axis, while the rate of 4-MU formation by labeled alkaline phosphatase is plotted on the vertical axis. In addition, the results of examining the correlation between a conventional HPLC method and this 10-minute assay system using the same patient samples as described in Example 2 are shown in Fig. 4. The measurement results are for 50 patient specimens. HbA1c % concentrations as determined with the conventional method (HLC-723GHbIII) are plotted on the horizontal axis, while the results determined according to the method of the present invention are plotted on the vertical axis. The correlation with the HPLC method was good, yielding a correlation equation of y = 0.579x + 1.125 and a correlation coefficient r = 0.933. In addition, the results of examining the intra-assay reproducibility in this 10-minute assay system in the same manner as the 40-minute measurement system shown in Example 3 are shown in Table 3, while the results of examining inter-assay reproducibility are shown in Table 4. HbA1c as determined by HPLC (%) 4.50 6.10 8.20 10.00 Measured values (%) 4.10 5.67 6.65 7.26 4.43 5.54 6.52 7.27 4.56 5.40 6.37 7.61 4.65 5.60 7.05 7.61 4.23 5.48 6.42 7.02 4.51 5.98 6.5 7.31 4.58 5.44 6.54 7.27 4.26 5.25 6.52 7.25 4.37 5.86 6.32 7.56 4.49 5.50 6.61 7.13 Mean 4.42 5.57 6.55 7.33 Standard deviation 0.18 0.22 0.20 0.20 Coefficient of variation CV (%) 4.01 3.89 3.10 2.74 HbA1c as determined by HPLC (%) 4.80 10.3 Measured values (%) 4.65 7.51 4.83 7.64 4.73 7.57 4.64 7.63 4.70 7.43 Mean 4.71 7.56 Standard deviation 0.08 0.09 Coefficient of variation CV (%) 1.62 1.16 Good results were obtained, demonstrating intra-assay reproducibility of 2.7 to 4.0% and inter-assay reproducibility of less than 4%. The correlation between the 40-minute system shown in Example 2 and the 10-minute assay system shown in the present Example was also good, exhibiting a correlation equation of y = 0.493 + 0.721x and r = 0.921. Example 5. Reactivity with Hemoglobin Variants Measurement of glycosylated hemoglobin variants concentration was attempted using the method of the present invention described in Example 1 for six types of hemoglobin variants acquired (UBE2, HAMADEN, TAKAMATSU, CAPE TOWN, RIYADH and HIKARI; UBE2 and CAPE TOWN are α-chain variants, while the others are β-chain variants). Those results are shown in Table 5. Name Results as measured according to present invention (40 minute system) (HbA1c%) UBE2 4.62 HAMADEN 2.13 TAKAMATSU 0 CAPE TOWN 0.22 RIYADH 0.42 HIKARI 0.78 The results show that these hemoglobin variants are not recognized at all in the present method, thereby indicating that the present method is specific for normal HbA1c only, and that it is not affected at all by the presence of hemoglobin variants. Example 6. Comparison of Pretreatment Solutions After adding 580 µl of pretreatment solution A in 0.1 M succinate buffer (pH 5.03) containing 0.1% Triton X-100 and 0.1% NaN 3 , or 580 µl of pretreatment solution B in distilled water (pH 7.15) containing 0.1% Triton X-100 and 0.1% NaN 3 , to 20 µl of whole blood samples obtained from normal adults using EDTA as anticoagulant followed by stirring, the mixtures were allowed to stand for 30 minutes at room temperature to complete hemolysis. These solutions were then used as the sample solutions for the immunoassay. One-step immunoassay was then performed on the AIA-1200 in the same manner as in Example 4 to determine the concentrations of HbA1c. The results are shown in Table 6. Pretreatment solution Rate of 4-MU formation 1st measurement 2nd measurement Mean A 134.441 148.458 141.450 B 3.949 4.355 4.152 As is clear from Table 6, performing pretreatment under weakly acidic condition results in the obtaining of a higher rate of 4-MU formation. Furthermore, HbA1c concentration in the whole blood specimen was measured to be 5.4% with a glycohemoglobin analyzer (HLC-723GHbIII, Tosoh).

Claims: A method for measurement of hemoglobin A1c comprising the steps of bringing a sample containing hemoglobin A1c to be measured into simultaneous contact with a solid phase and a labeled anti-hemoglobin A1c antibody resulting in binding of the labeled anti-hemoglobin A1c antibody to the hemoglobin A1c which is immobilized on the solid phase, separating the resulting immobilized labeled anti-hemoglobin A1c antibody from labeled anti-hemoglobin A1c antibody in solution, and detecting the label of the immobilized anti-hemoglobin A1c antibody. The method according to claim 1 wherein a pre-treatment of said sample is performed under weakly acidic conditions. The method according to claim 2 wherein said weakly acidic conditions refer to a pH of 3.0 to 6.9. The method according to claim 2 or 3 wherein an aliphatic carboxylate buffer or borate buffer is used for pre-treatment of said sample. The method according to claim 4 wherein said aliphatic carboxylate buffer is succinate, malonate, fumarate, maleate, glutarate or acetate buffer. The method according to any one of claims 2 to 5 wherein said sample is a blood sample and hemolysis of the sample is performed prior to or simultaneously with the pre-treatment.

Inventors: INOUE MASUO, MARUO NAOKO, INOUE, MASUO, MARUO, NAOKO
Applicants: TOSOH CORP, TOSOH CORPORATION
Application year: 1997
Main classification: G01N33
IPC codes: G01N33