Capsule containing whey protein

Abstract: A capsule has a capsule membrane containing a Process Whey Protein together with at least one selected from scleroproteins, derived proteins and mucopolysaccharides. The capsule membrane may contain a plasticizer.

Description: BACKGROUND OF THE INVENTION The present invention relates to a capsule which may be stable even if it has a thin membrane, which is superior in collapsing property but causes substantially no caking nor deformation by change of pressure, temperature, humidity or the like and which can be useful in foods, drugs and cosmetics and is high in safety. Capsule products have-been conventionally used in various fields such as foods, drugs and cosmetics. However, the capsule membrane has been prepared mainly by mixing a hydrated and swollen gelatin with a plasticizer and then drying the mixture. Such a capsule can be prepared relatively easily and is high in transparency and excellent in beauty. However, it has many disadvantages as follows. (1) It requires a long period for the preparation and drying of the membrane material in its production. (2) It requires strict temperature control to avoid leakage of packed suspension, formation of so-called a vec sphere by mutual adhesion of capsules and deformation. (3) In the storage of capsules, a low humidity causes lowering of strength of the membrane to easily damage it by impact. (4) A high humidity, a high temperature or shortage of releasing agent causes deformation and caking. (5) If the thickness of-the membrane is made thin, the strength becomes insufficient. (6) The collapse in digestive organs is slow. On the other hand, as a method for eliminating these disadvantages of capsule membrane, JP 02-22221 A discloses a method for the preparation of a capsule improved in sliding and collapsing property by using a natural calcium material. However, this method reduces transparency of the capsule and cannot produce a product of excellent beauty and cannot fully accomplish reduction of manufacturing period, improvement in strength and prevention of avec sphere and deformation, disadvantageously. An object of the present invention is to eliminate disadvantages of conventional capsules as described above and to provide a capsule which can be easily end stably prepared and prevents liquid leakage and occurrence of avec sphere and deformation and is rich in membrane strength and has a high stability against changes in pressure, temperature and humidity. Further, an object of the present invention is to make the capsule membrane thin and to make the size of the capsule small and as the result to give a capsule product which can be easily dosed and is high in collapsing property and softness and can be used widely in various fields such as foods, drugs and cosmetics and is superior in safety. SUMMARY OF THE INVENTION The present invention aims to overcome or mitigate the above problems by forming a capsule membrane containing "Process Whey Protein". In accordance with the present invention there is provided a soft capsule in which a capsule membrane contains a Process Whey Protein together with at least one compound or substance selected from scleroproteins, gelatin, low molecular weight gelatin, gelatin hydrolysate and mucopolysaccharides, the Process Whey Protein having been prepared by removing substantially all low molecular weight compounds other than milk whey protein from milk whey to obtain a residual product and heat-treating the residual product at a pH not higher than 4 or not lower than 6. The capsule membrane may further contain a plasticizer. DETAILED DESCRIPTION OF THE INVENTION "Process Whey Protein" used in the present invention is prepared by a procedure in which low molecular compounds other than milk whey protein are substantially removed from milk whey which is a waste material from the manufacture of cheese, butter and case in by using milk as the raw material and then the pH of the product is adjusted to not higher than 4 or not lower than 6 and then heat-treated. For example, by using cheese whey formed in the manufacture of cheese, the low molecular compounds in milk whey are removed substantially by dialysis or chromatography and the product is heat-treated at a pH not higher than 4 or not lower than 6 to give the Process Whey Protein. For example, the method for the preparation of "Process Whey Protein" is-disclosed in US 5416196 A and EP 0473270 B which are corresponding to JP 04-228036 A, JP 04-267850 A and JP 06-292514 A. The commercial products include "Genesis (trade name)" manufactured by Daiichi-Kasei Co., Ltd.. Such Process Whey Protein has many functional characteristics which cannot be obtained by other protein materials. For example, it gives a transparent viscous oily liquid showing Newtonian behaviour, a transparent sol and a transparent gel. Particularly, a transparent gel prepared by adding a salt and heating is not molten even when heated to as high a temperature as in a retort. A high temperature is not especially required for the preparation of sol or gel and it is enough to be 0°C or higher. A gel can be formed even at room temperature. Furthermore, Process Whey Protein does not gel by heating when no salt nor mineral is added to form a transparent viscous liquid. Also, Process Whey Protein is a protein material excellent in emulsification stability and digestive property and high in effective nutritive value. As the "scleroprotein" constituting the main components of the capsule membrane of the present invention, useful are collagen, collagen hydrolyzate and the like. As the "derived protein", useful are gelatin, low molecular gelatin, gelatin hydrolyzate and the like. As the "mucopolysaccharide", useful are chondroitin sulfate and hyaluronic acid. They can be used each alone or in combination. Particularly, gelatin is preferred for taste. The "plasticizer" is not particularly restricted but the useful compounds are glycerol, polyglycerol, polyethylene glycol, propylene glycol and the like. They can be used each alone or in combination. The used amount of "Process Whey Protein" is not especially restricted but is preferably 0.07 to 2 weight %, more preferably 0.1 to 1 weight %, based on the solid amount of the capsule membrane. Usually, a solution containing 7 to 10 weight % of Process Whey Protein is added in an amount of 1 to 20 weight %, preferably 1 to 10 weight %, based on the total amount of the raw materials for capsule membrane before drying for use. The used amount of scleroproteins, derived proteins and mucopolysaccharides used is also not particularly restricted but usually about 60 to 99 weight % in the capsule membrane is preferred. It is preferably 5 to 50 weight % based on the total amount of the raw materials before drying. The amount of the plasticizer used in the present invention may be 0 to 40 weight %, particularly 5 to 35 weight %, of the capsule membrane and usually it is preferred to use 10 to 30 weight % based on the total amount of the raw materials before drying. The capsule of the present invention can be prepared by mixing a solution of Process Whey Protein with other materials and heating and drying it. The mixing can be carried out by dissolving or dispersing uniformly for example with stirring according to usual method. A jacketed stirring defoaming vessel can be used for simultaneously carrying out deaeration, stirring and heating. The heating temperature for the mixture is preferably 50 °C or higher, particularly 70 to 100°C. The succeeding drying process requires no special means and can be carried out by a usual method, for example by using an air flow type rotary dryer. The moisture after dried is preferably 3 to 10 weight %, particularly 4 to 8 weight %. The Process Whey Protein used in the present invention is prepared by heat-denaturing whey protein, which is a globular protein, under a specific condition to modify it to "a soluble linear aggregate". When no salt is added, the Process Whey Protein aggregates repel each other by static repulsion and therefore it does not gel nor thickens when heated. However, when a salt is added, static repulsion between aggregates is weakened and the aggregates approach each other by intermolecular attraction and hydrophobic interaction and then a fine heat-irreversible gel network is formed by disulfide bond and S-S exchange. In the present invention, we have found that a gel network and a membrane can be easily formed by heat-drying Process Whey Protein even when no salt is added. As the result of utilizing the property of Process Whey Protein to improve the property of capsule product, we have found that a capsule prepared by adding at least one of scleroproteins, derived proteins and mucopolysaccharides to Process Whey Protein and further adding at least one plasticizer such as glycerol and then heating and drying has a far more excellent property than conventional ones. Process Whey Protein forms a rigid gel and a membrane network by decreasing moisture content even when no salt is added and this fine heat-irreversible gel network can be formed even in a system where other proteins and polysaccharides are present to enhance total strength and heat-resistance of the gel. From the reason, when used in the capsule membrane of the present invention, the membrane structure is rapidly of the membrane is increased with no loss of transparency and the adhesion at the time of capsule formation is improved and also the formation of avec sphere and deformation can be prevented. This characteristic acts highly advantageously in the capsule forming process to make possible adhesion at a low temperature and to make thin the membrane thickness. It also acts positive on several properties of the prepared capsule including collapsing property in water, strength against compression and drying, and melting property by heating. The soft capsule prepared according to the present invention can reduce the drying period and can improve the condition and the period for maintaining the quality of the produce capsule and is beneficial to the view point of energy saving and effective use of resources. PREFERRED EMBODIMENTS OF THE INVENTION The following examples serve to illustrate the invention. The chemicals, capsule-forming methods and test methods used in the examples are as follows. [Capsule material] A Process Whey Protein solution ( "Genesis" manufactured by Daiichi Kasei Co., Ltd., Process Whey Protein content: 9 % ), glycerol ( manufactured by Nippon Yushi Co., Ltd. ) and hot water are mixed together in a stirring defoaming vessel in which hot water is passed through the jacket, and gelatin ( manufactured by Nitta Glelatin Co., Ltd. ) is added and dissolved into the mixture, and then the mixture is defoamed in vacuo , and the viscosity was adjusted ( by adding hot water when the viscosity is high, and by repeating the defoaming operation when it is low ) and then the resultant solution is stored in a subdividing tank for about 6 hours ( at 55 to 60°C ) for aging. [Packing liquid] Soybean oil - Purified soybean oil ( manufactured by Linol Yushi Co., Ltd. ) is filtered ( 50 mesh ), or Chitosan suspension - 1620 g of safflower oil ( manufactured by Linol Yushi Co., Ltd. ), 90 g of beeswax ( manufactured by Serarika NODA Co., Ltd. ) and 90 g of Poem S-100 ( manufactured by Riken Vitamin Co., Ltd. ) are dissolved at 70°C by heating and then the liquid temperature is lowered to about 45°C, and 1000 g of chitosan ( manufactured by Kimitsu Kagaku Kogyo Co., Ltd. ) and 200 g of soybean lecithin ( True Lecithin Kogyo Co., Ltd. ) are added and the mixture is mixed by using a T.K.HOMOMIXER ( manufactured by Tokushu Kika Kogyo Co., Ltd. ) ( 10000 rpm, 20 minutes, 40°C ) and then the solution is made into corpuscles or fine particles by using a T.K.MYCOLLOIDAR ( manufactured by Tokushu Kika Kogyo Co., Ltd. ) and filtered ( 50 mesh ) and defoamed in vacuo . [Capsule-forming method] It was formed by a usual method with use of a rotary capsule-forming machine ( manufactured by Kamata Co., Ltd. ). The used mold was OVAL type or OBLONG type. [Test of physical properties of the resultant preparation] Appearance inspection - By visual observation. Moldability - Assessed by an experienced operator. Viscosity of capsule material - By a B type viscometer (manufactured by Tokyo Keiki Co., Ltd. ), Collapsing test - Tested in accordance with J.P. collapsing test method. Membrane moisture - Tested in accordance with J.P. drying loss test method by using an infrared moisture meter ( manufactured by Kett Co., Ltd., FD-230 type ). Rupture load ( strength against compression ) - Tested by using a Kiya type hardness tester ( manufactured by Fujiwara Seisakusho Co., Ltd., maximum load: 30 kg ). Stability test ( accelerated ) - Carried out by using a constant temperature constant moisture vessel ( manufactured by Advantec Co., Ltd., AGX-226 ) and the extent of caking was observed. Example 1 By using the above-mentioned capsule material containing a Process Whey Protein solution and using a soybean oil or a chitosan suspension as the packing liquid, various type capsules A to K including OVAL type and OBLONG type as shown in Table 1 were prepared. As the result, in all conditions, those using Process Whey Protein, gelatin and glycerol as the capsule material gave good capsule formation. From these test results, it was apparent that the capsule using Process Whey Protein had an appearance and moldability equivalent to the conventional product. Comparative Example 1 Capsules C' to I' were prepared in the same manner as for capsules C to I in Example 1 except that a capsule material containing no Process Whey Protein solution was used. Comparison tests 1 to 6 Physical properties of the products prepared in Example 1 were compared to those prepared in Comparative Example 1 or conventional products. [Comparison test 1] The lowest-temperature of the segment (heat supply equipment for the sheet) which can provide good adhesion was measured and the result is shown in Table 2. Adhesive property of sheet Sample E E' F F' Mold (No. 5) OVAL OVAL OBLONG OBLONG Glycerol (wt. parts) 50 50 50 50 Sheet thickness (mm) 0.50 0.50 0.50 0.50 PWP (wt. parts) 5 0 5 0 Segment temperature (°C) > 31 > 34 > 32 > 36 The products ( E, F ) of Examples in accordance with the present invention gave segment temperatures lower than those of the products ( E', F' ) of Comparative Example by 3 to 4°C. From the result, it was found that a capsule material prepared from Process Whey Protein, gelatin and glycerol can be adhered at a lower temperature than the conventional one and can avoid generation of avec sphere and deformation to improve adhesion property largely. [Comparison test 2] The capsule material was overdried and the rupture load at compression was measured. The result is shown in Table 3. Capsule strength when overdried Sample D D' G G' Mold (No. 5) OVAL OVAL OBLONG OBLONG Glycerol (wt. parts) 50 50 50 50 Sheet thickness (mm) 0.50 0.50 0.50 0.50 PWP (wt. parts) 10 0 10 0 Membrane moisture (%) 4.5 4.3 4.0 4.3 Rupture load (kg) 25 14 27 15 PWP: 9 % aqueous solution of Process Whey Protein The products ( D, G ) of Example in accordance with the present invention were found to provide significantly higher strength than the products ( D', G' ) of Comparative Example and hardly broken. From the result, it was found that a capsule material using Process Whey Protein can prepare a thinner capsule than the conventional product. [Comparison test 3] A capsule was prepared by increasing the plasticizer to give softness and it was stood at 50°C and at 40°C and at a relative humidity of 75 % and its property was examined. The results are shown in Tables 4 and 5. From these results, it was found that the products ( D, G ) of Example according to the present invention hardly cake and are high in stability against heat and humidity compared to the products ( D', G' ) of Comparative Example. Heat stability when the plasticizer is increased (50°C, closed vessel). Sample D D' G G' Mold (No. 5) OVAL OVAL OBLONG OBLONG Glycerol (wt. parts) 50 50 50 50 Sheet thickness (mm) 0.50 0.50 0.50 0.50 PWP (wt. parts) 10 0 10 0 Membrane moisture (%) 5.3 5.3 5.4 5.3 Caking 1 hour - - - - 2 - - - + 3 - + - + 4 - + - + 5 - + - + 6 - + - ++ 7 - + - ++ 8 - + - ++ PWP: 9 % aqueous solution of Process Whey Protein Criteria -: No caking. ( Detached by low impact ) + : Slight caking. ( Somewhat difficult to be broken by low impact ) ++: Intermediate caking. ( Remarkable mass though somewhat broken by low impact ) Temperature stability of capsule when the plasticizer is increased (temperature: 40 °C, humidity: 75 %, open vessel). Sample D D' G G' Mold (No. 5) OVAL OVAL OBLONG OBLONG Glycerol (wt. parts) 50 50 50 50 Sheet thickness (mm) 0.50 0.50 0.50 0.50 PWP (wt. parts) 10 0 10 0 Membrane moisture (%) 5.3 5.3 5.4 5.3 Caking 1 hour - - - - 2 - - - - 3 - + - + 4 + ++ + + 5 ++ +++ + ++ 6 +++ +++ ++ +++ 7 +++ +++ +++ +++ PWP: 9 % aqueous solution of Process Whey Protein Criteria -: No caking. ( Detached by low impact ) +: Slight caking. ( Somewhat difficult to be broken by low impact ) ++: Intermediate caking. ( Remarkable mass though somewhat broken by low impact ) +++: Heavy caking ( Capsule is not broken by impact ) [Comparison test 4] The collapsing property of the capsule with water was investigated. The result is shown in Table 6. It was found that the products ( C, I ) of Example according to the present invention require longer period until collapsing ( opening ) than the products ( C', I' ) of Comparative Example, but collapsed in shorter period than those of Comparative Example. From the result, it was found that the capsule material prepared from Process Whey Protein, gelatin and glycerol could give a capsule which has an excellent property that it was difficult to be broken in the mouth and collapsed more rapidly in digestive organs. Collapsing property of capsule against water (37°C) Sample C C' I I' Mold (No.5) OVAL OVAL OBLONG OBLONG Glycerol (wt. parts) 35 35 50 50 Sheet thickness (mm) 0.85 0.85 0.85 0.850 Packing liquid Soybean oil Chitosan PWP (wt. parts) 5 0 10 0 Opening (minutes) 2∼3 1∼2 1∼2 2∼3 Collapsing (minutes) 6∼7 7∼11 6∼8 8∼11 [Comparison test 5] The relationship between sheet thickness (membrane thickness) of the capsule and drying period was tested. The result is shown in Table 7. The products ( G, H ) of Example according to the present invention could be prepared stably to a sheet thickness of 0.3 to 0.5 mm but the capsule material of the conventional product was difficult to prepare such a thin membrane. Also, the period required for drying the capsule material of the products ( G, H ) according to the present invention came to be far shorter as the sheet could be made thinner than the conventional product. From these results, it was found that the capsule material prepared from Process Whey Protein, gelatin and glycerol could be made thinner than the conventional product and therefore the capsule form could be made smaller and thus it could be dosed easily and it could collapse more rapidly in digestive tracts and hence the packed material could be exposed in the digestive tracts in a shorter time, that is, it gave an immediate effect. Also, as the sheet can be made thinner in the preparation of capsule, the drying period can be reduced to improve productivity. Sheet thickness of capsule and drying period Sample G H Conventional product Mold (No. 5) OBLONG OBLONG OBLONG Glycerol (wt. parts) 50 50 35 Sheet thickness (mm) 0.50 0.30 0.85 PWP (wt. parts) 10 5 0 Membrane moisture (%) Drying period 1 hour 13.1 9.0 17.7 3 hours 9.8 7.0 11.6 5 hours 8.5 6.2 10.5 24 hours 6.0 4.7 8.7 48 hours 5.4 4.3 7.3 Example 2 A bath capsule was prepared in the same manner as in Example 1 and the properties were evaluated in the same manner as in Example 1. The mold used was No.90 ROUND for bath capsule. The test result for physical properties of a bath capsule (L) prepared by using Process Whey Protein, gelatin and glycerol according to the present invention is shown in Table 8 compared with a conventional product. While preparation of a practically usable capsule was difficult at a sheet thickness of 0.65 mm by using the conventional material, the product (L) using the Process Whey Protein could be made to a sheet thickness of 0.65 mm. Also, it was found that the product (L) of the present invention is difficult to cake compared to the conventional product and also is good in collapsing property. This feature is useful as a bath capsule. Characteristics of bath capsule prepared by using Process Whey Protein L Conventional product Capsule material Gelatin (wt. parts) 100 100 Glycerol (wt. parts) 52 50 PWP (wt. parts) 10 - Sheet thickness ( mm ) 0.65 0.90 Capsule form (No. 90 mold) ROUND ROUND Packing liquid Soybean oil Packed amount ( mg ) 6000 6000 Capsule material moisture ( %, 24 hours ) 6.8 7.2 Transparency Transparent Collapsing period(40°C, min.) < 1 < 2 Example 3 By using low molecular gelatin ( manufactured by Nitta Gelatin Co., Ltd., 114 Brume ) said generally to be unsuitable for the preparation of capsule, capsules ( M, N, O ) were prepared in the same manner as in Example 1 and, at the same time, capsules ( M', N', O' ) of Comparative Example using no Process Whey Protein in the capsule material were prepared. The property of these capsules is shown in Table 9. From the result, it was found that, while the products ( M, N, 0 ) according to the present invention gave capsules which had a sheet thickness of 0.50 to 0.85 mm and was transparent and excellent in collapsing property by water and showed high strength, the products ( M', N', O' ) of Comparative Example prepared by not using Process Whey Protein was unmoldable and showed a property difficult in practical production. From this result, a relatively high molecular gelatin has been usually used for the preparation of capsule. However, the high molecular gelatin has defect in solubility and loses viscosity remarkably by hydrolysis immediately after the preparation and therefore an aging process is essential for a long period and also the viscosity decreases during use thereafter and fine adjustment of forming condition is unavoidable. Contrary to it, low molecular gelatin is excellent in solubility but is low in apparent viscosity and is very low in forming aptitude of capsule. A capsule material prepared by using Process Whey Protein and low molecular gelatin is high in solubility and minute in viscosity decrease as the time passes and high in forming aptitude. From these facts, it was found that the aging process could be largely reduced to improve productivity. Properties of capsules prepared by using low molecular gelatin M M' N N' O O' Capsule material LM gelatin(wt.parts) 100 100 100 100 100 100 Glycerol(wt.parts) 50 52 50 50 50 50 PWP(wt.parts) 10 0 10 0 10 0 Viscosity ( x 1000 cps) 20 10 10 10 10 10 Sheet thickness (mm) 0.30 0.30 0.50 0.50 0.50 0.50 Capsule form (No. 5 mold) OBLONG OBLONG OBLONG OBLONG OBLONG OBLONG Packing liquid Soybean oil Chitosan Packed amount (mg) 250 250 250 250 250 250 Capsule material moisture (%, 48 hours) 4.3 4.7 4.5 4.7 Membrane breaking load(kg) 15 23 14 22 Transparency Transparent Transparent Collapsing period (min) 1∼2 1∼4- 2∼4 2∼5 Moldability Δ × o ○ Δ o ○ × Example 4 Capsules were prepared in the same manner as in Example 1 by using no plasticizer and using a capsule material consisting of Process Whey Protein, gelatin and water. While a capsule using no plasticizer could not be prepared by the prior art, the method of the present invention gave capsules ( P, Q ) excellent in transparency an high in forming aptitude as shown in Table 10 even with no addition of a plasticizer. Properties of capsules prepared by using no plasticizer P Q Capsule material Geratin (wt.parts) 100 100 PWP (wt.parts) 15 20 Viscosity(x1000cps) 18 18 Sheet thickness(mm) 0.85 0.85 Capsule form (No.5) OVAL Packing liquid Soybean oil Packed amount (mg) 250 Transparency Transparent Moldability Normal PWP: 9 % solution of Process Whey Protein Example 5 Capsules were prepared in the same manner as in Example 1 by using polyethylene glycol ( manufactured by Nippon Yushi Co., Ltd., #400 ) or polyglycerol ( manufactured by Sakamoto Yakuhin Kogyo Co., Ltd., #500 ) as the plasticizer. As shown in Table 11, the present invention could prepare products excellent in transparency and forming property even in the case polyethylene glycol or polyglycerol was used as the plasticizer. Properties of capsules prepared by using polyethylene glycol and polyglycerol R S V W Capsule material Gelatin (wt.parts) 100 100 100 100 PEG (#400) (wt.parts) 20 30 50 0 Polyglycerol (#500) (wt.parts) 0 0 0 30 PWP (wt.parts) 20 10 5 10 Viscosity (× 1000cps) 15 15 15 20 Sheet thickness (mm) 0.85 0.85 0.85 0.85 Capsule form (No. 5 mold) OVAL Packing liquid Soybean oil Packed amount (mg) 250 Transparency Somewhat turbid Transparent Moldability Good Normal According to the present invention, it became possible to prepare a capsule membrane for foods, drugs and cosmetics which has a highly improved adhesion of membrane sheet used in the preparation of capsules and is formable at a lower temperature and can avoid generation of avec sphere and deformation and scarcely broken under an overdried condition as the membrane strength is increased and is stable against heat and moisture and difficult to cake and excellent in collapsing property in digestive tracts. Also, in the present invention, it came possible to prepare a capsule which has thin membrane and is smaller and easy to be taken and rapidly digested and softer and difficult to cake and also a product containing no plasticizer. Furthermore, according to the present invention, it came possible to use a protein material which has been difficult to be used conventionally as the capsule material and to save the resources, time and energy required for the preparation of capsules.

Claims: A soft capsule in which a capsule membrane contains a Process Whey Protein together with at least one compound or substance selected from scleroproteins, gelatin, low molecular weight gelatin, gelatin hydrolysate and mucopolysaccharides, the Process Whey Protein having been prepared by removing substantially all low molecular weight compounds other than milk whey protein from milk whey to obtain a residual product and heat-treating the residual product at a pH not higher than 4 or not lower than 6. A soft capsule according to Claim 1, in which the capsule membrane contains a plasticizer. A soft capsule according to Claim 2, in which the plasticizer is at least one selected from glycerol, polyglycerol, polyethylene glycol and propylene glycol. A soft capsule according to any of Claims 1 to 3, in which the capsule membrane contains said Process Whey Protein in an amount of 0.07 to 2 weight % on dry basis. A soft capsule according to any of Claims 1 to 4, in which the capsule membrane contains said at least one compound or substance in an amount of 60 to 99 weight % on dry basis. A soft capsule according to any preceding claim, in which the moisture content of the capsule membrane is 3 to 10 weight %. A soft capsule according to any preceding claim, being transparent and free from plasticizer. A soft capsule according to any of claims 1 to 8, being a moulded capsule.

Inventors: KATAGIHARA HIROSHI, KINEKAWA YOHICHI, KITABATAKE NAOFUMI, SOGAWA SATOSHI, UEDA YOSHINORI, YONEMOTO YUKIKO, KATAGIHARA, HIROSHI, KINEKAWA, YOHICHI, KITABATAKE, NAOFUMI, SOGAWA, SATOSHI, UEDA, YOSHINORI, YONEMOTO, YUKIKO
Applicants: ALIMENT INDUSTRY CO LTD, DAIICHI KASEI, ALIMENT INDUSTRY CO., LTD., DAIICHI KASEI CO., LTD.
Application year: 1997
Main classification: A61K9
IPC codes: A61K47, A61K9, A61K8, A23P1, A61Q19