THERAPEUTIC USES OF BPI PROTEIN PRODUCTS IN HUMANS WITH HEMORRHAGE DUE TO TRAUMA

Abstract: Methods and materials for the treatment of humans suffering from hemorrhage due to trauma are provided, in which therapeutically effective amounts of BPI protein products are administered.

Description: THERAPEUTIC USES OF BPI PROTEIN PRODUCTS IN HUMANS WITH HEMORRHAGE DUE TO TRAUMA This is a continuation-in-part application of U.S. Serial No. 08/652,292, filed May 23, 1996, incoφorated herein by reference. BACKGROUND OF THE INVENTION The present invention relates generally to methods and materials for treating humans suffering from hemorrhage due to trauma, by administration of bactericidal/permeability-increasing (BPI) protein products. Acute traumatic hemorrhage, generally requiring immediate surgical intervention, is a major contributor to morbidity and mortality in the U.S. [Bickell et al., New Eng. J. Med. , 331: 1105-1109 (1994), Tran et al., Surgery, 114:21-30 (1993).] In 1982, there were approximately 165,000 deaths in the U.S. due to trauma, with at least two additional cases of permanent disability for each death. About 50% of these traumatic deaths occur immediately, due to direct injury to the central nervous system, heart, or one of the major blood vessels. Additional early deaths, approximately 30%, occur within several hours after injury, usually due to uncontrolled hemorrhage. The remaining 20% of deaths are so-called "late deaths", occurring during days to weeks after injury, due to complications from the traumatic hemorrhage that include infection or multiple organ system failure (MOSF) in about 80% of the cases. [Trunkey, Sci. Am. , 249:28-35 (1983), Trunkey, New Eng. J. Med., 324:1259-1263 (1991).] Among those patients who survive the immediate resuscitative and surgical interventions, approximately 10-40% suffer from a variety of morbidities, including, for example, systemic inflammation, wound infections, pneumonia, sepsis, respiratory failure, renal failure, coagulopathy, and pancreatitis. Hemorrhage and transfusion requirements may be specifically linked to increased risk of postoperative infection, respiratory complications, and multiorgan system failure [Agarwal et al., Arch. Surg. , 225:171-177 (1993), Duke et al., Arch. Surg. , 725: 1125-1132 (1993), Tran et al., supra]. The causes of these complications from traumatic hemorrhage are multifactorial and interrelated. Many morbidities may be related to systemic inflammation following injury. It has also been hypothesized that physical trauma to tissue, direct tissue hypoperfusion, and translocation of endogenous bacteria and absorption of endotoxin from the gut lumen (due to hypoperfusion and/or other injury to the gastrointestinal tract) may play a role in the pathogenesis of these complications. The relevance of these proposed factors in the pathophysiology of the morbidities and late deaths associated with acute hemorrhagic shock in humans, however, is not clear. Although acute traumatic hemorrhage is one potential cause of hypovolemic shock (i.e. , shock due to decreased intravascular volume), there are numerous other potential causes, such as internal bleeding, e.g. , gastrointestinal hemorrhage, intraperitoneal or retroperitoneal hemorrhage, hemorrhage into the femoral compartment, intrathoracic hemorrhage, aortic dissection and ruptured aortic aneurysm; excessive fluid loss due to, e.g. , severe vomiting due to an intestinal or pyloric obstruction, severe diarrhea, sweating, dehydration, excessive urination (due to diabetes mellitus, diabetes insipidus, excessive diuretics, or the diuretic phase of acute renal failure), peritonitis, pancreatitis, planchnic ischemia, gangrene, burns; vasodilation due to, e.g. , nervous system damage, anesthesia, ganglionic and adrenergic blockers, barbiturate overdose, poisons; and metabolic, toxic, or humoral vasodilatation, such as acute adrenal insufficiency, or an anaphy lactic reaction. Other causes of shock unrelated to circulatory volume loss include cardiogenic shock (e.g. , acute myocardial infarction, cardiac tamponade) and obstructive shock (e.g. , acute pulmonary embolism). [See, e.g., Manual of Medical Therapeutics, 28th ed., Ewald et al., eds., Little, Brown and Company, Boston (1995); Cecil's Textbook of Medicine, 17th ed., Wyngaarden et al. , eds., W.B. Saunders Co., Philadelphia (1985).] As outlined below in Table I below, a normal individual can rapidly lose up to 20 per cent of the blood volume without any signs or symptoms. Limited signs of cardiovascular distress appear with losses up to 30 per cent of the blood volume, but signs and symptoms of hypovolemic shock generally appear when the blood loss exceeds 30 to 40 per cent of the blood volume. Table I The patient is frequently oliguric, with a urinary output of less than 20 mL per hour. Frequently, the physical findings follow a progressive pattern as shock evolves from the early compensated phase to the advanced stages. In Stage I, physiologic compensatory mechanisms, such as increased cardiac output or elevated systemic vascular resistance, are effective and minimal clinical symptoms and signs are observed. In Stage π , these mechanisms cannot effectively compensate for the blood volume loss, and the patient may exhibit hypotension, tachycardia, and hyperventilation. The decreased perfusion of vital organs can result in an altered mental state ranging from agitation to stupor to coma, reduced urinary output, and myocardial ischemia (in patients with coronary artery disease). The external appearance of the patient also reflects excessive sympathetic discharge, with cyanosis, coldness, and clamminess of the skin. In Stage HI, which may be irreversible, the excessive and prolonged reduction of tissue perfusion leads to significant alterations in cellular membrane function, aggregation of blood corpuscles, and "sludging" in the capillaries. The vasoconstriction which has taken place in the less vital organs in order to maintain blood pressure in now excessive and has reduced flow to such an extent that cellular damage occurs. Following traumatic hemorrhage, conventional therapy is directed at stopping the hemorrhage, combating shock, and restoring the blood volume. Prompt fluid resuscitation is preferably given through large-bore catheters placed in large peripheral veins. The pneumatic antishock garment, with sequential inflation of legs and abdominal compartments to 15-40 mm Hg, may temporally stabilize patients by increasing peripheral systemic vascular resistance. Restoration of the blood volume may be achieved by intravenous infusion of electrolyte solutions; colloid solutions of plasma protein, albumin, or dextran; or fresh whole blood. In the emergency situation, electrolyte solutions, albumin, or dextran are preferred over fresh whole blood because of the large amounts of fluid required, the possible delay in transfusion if typing and cross-matching are performed, and the possibility of allergic transfusion reactions. When shock is due to hemorrhage, packed red blood cells should be given as soon as feasible. When hemorrhage is massive, type-specific unmatched blood can be given safely. Rarely, type O blood may be needed. Rapid infusion of Ringer's lactated or normal saline solution is the most widely used fluid therapy following hemorrhage. An initial infusion of two to three times the volume of the estimated blood loss is administered. Because these solutions are rapidly distributed throughout the intravascular and extravascular compartments, they must be supplemented with colloid solutions. When large volumes of electrolyte solutions are infused, patients often develop peripheral edema and elderly patients may develop pulmonary edema. The colloidal preparations in wide use include a 6 per cent solution of high molecular weight dextran (dextran 70), a 10 per cent solution of low molecular weight dextran (dextran 40), and a 5 per cent solution of albumin in normal saline. Infusions of dextran 70 produce an initial volume effect slightly greater than the amount infused. Dextran 70 is slowly cleared over one to two days, allowing time for normal physiologic mechanisms to replace the volume lost. Dextran 40 has the advantage of an initial volume effect of nearly twice the amount infused. The lower molecular weight material is more rapidly cleared, however, and the volume- expanding effect is dissipated by 24 hours, before normal volume replacement mechanisms are maximal. Acute renal failure has occurred in a few patients receiving dextran 40. With either dextran solution, volumes in excess of one liter may interfere with platelet adhesiveness and the normal coagulation cascade. A solution of 5 per cent albumin in normal saline has the advantage of producing a known volume effect in the hypovolemic patient, but this preparation is relatively costly and time-consuming to prepare. A hypertonic albumin preparation containing 120 mEq of sodium lactate, 120 mEq of sodium chloride, and 12.5 grams of albumin per liter provides a predictable volume effect and minimizes interstitial fluid leakage. Use of hypertonic solutions requires careful monitoring of arterial and central venous pressures to avoid fluid overload. Coexisting problems such as congestive heart failure, valvular heart disease, myocardial ischemia, or renal insufficiency must be carefully monitored, and invasive hemodynamic monitoring must be considered during acute management. Associated coagulopathy and electrolyte imbalance must also be corrected. BPI is a protein isolated from the granules of mammalian polymorphonuclear leukocytes (PMNs or neutrophils), which are blood cells essential in the defense against invading microorganisms. Human BPI protein has been isolated from PMNs by acid extraction combined with either ion exchange chromatography [Elsbach, J. Biol. Chem., 254: 11000 (1979)] or E. coli affinity chromatography [Weiss, et al., Blood, 69:652 (1987)]. BPI obtained in such a manner is referred to herein as natural BPI and has been shown to have potent bactericidal activity against a broad spectrum of gram-negative bacteria. The molecular weight of human BPI is approximately 55,000 daltons (55 kD). The amino acid sequence of the entire human BPI protein and the nucleic acid sequence of DNA encoding the protein have been reported in Figure 1 of Gray et al. , J. Biol. Chem. , 264:9505 (1989), incorporated herein by reference. The Gray et al. amino acid sequence is set out in SEQ ID NO: 1 hereto. U.S. Patent No. 5,198,541 discloses recombinant genes encoding and methods for expression of BPI proteins, including BPI holoprotein and fragments of BPI. BPI is a strongly cationic protein. The N-terminal half of BPI accounts for the high net positive charge; the C-terminal half of the molecule has a net charge of -3. [Elsbach and Weiss (1981), supra.] A proteolytic N-terminal fragment of BPI having a molecular weight of about 25 kD possesses essentially all the anti-bacterial efficacy of the naturally-derived 55 kD human BPI holoprotein. [Ooi et al. , J. Bio. Chem. , 262: 14891-14894 (1987)]. In contrast to the N-terminal portion, the C- terminal region of the isolated human BPI protein displays only slightly detectable anti-bacterial activity against gram-negative organisms. [Ooi et al. , J. Exp. Med. , 174:649 (1991).] An N-terminal BPI fragment of approximately 23 kD, referred to as "rBPl23, " ^ as • Deen prod 006 ** Dv recombinant means and also retains anti-bacterial activity against gram-negative organisms. Gazzano-Santoro et al., Infect. Immun. 60:4754-4761 (1992). The bactericidal effect of BPI has been reported to be highly specific to gram-negative species, e.g., in Elsbach and Weiss, Inflammation: Basic Principles and Clinical Correlates, eds. Gallin et al. , Chapter 30, Raven Press, Ltd. (1992). The precise mechanism by which BPI kills gram-negative bacteria is not yet completely elucidated, but it is believed that BPI must first bind to the surface of the bacteria through electrostatic and hydrophobic interactions between the cationic BPI protein and negatively charged sites on LPS. In susceptible gram-negative bacteria, BPI binding is thought to disrupt LPS structure, leading to activation of bacterial enzymes that degrade phospholipids and peptidoglycans, altering the permeability of the cell's outer membrane, and initiating events that ultimately lead to cell death. [Elsbach and Weiss (1992), supra]. LPS has been referred to as "endotoxin" because of the potent inflammatory response that it stimulates, i.e., the release of mediators by host inflammatory cells which may ultimately result in irreversible endotoxic shock. BPI binds to lipid A, reported to be the most toxic and most biologically active component of LPS. BPI protein has never been used previously for the treatment of humans suffering from hemorrhage due to trauma or the shock associated with traumatic blood loss (i.e. , hypovolemic shock). Bahrami et al., presentation at Vienna International Endotoxin Society Meeting, August, 1992, report the administration of BPI protein to rats subjected to hemorrhage. Yao et al., Ann. Surg. , 222:398-405 (1995), report the administration of rBP^ j (described infra) to rats subjected to prolonged hemorrhagic insult for 180 minutes followed by resuscitation. U.S. Patent Nos. 5,171 ,739, 5,089,724 and 5,234,912 report the use of BPI in various in vitro and in vivo animal model studies asserted to be correlated to methods of treating endotoxin-related diseases, including endotoxin-related shock. In co-owned, co- pending U.S. Applicauon Serial Nos. 08/378,228, filed January 24, 1995, 08/291,112, filed August 16, 1994, and 08/188,221 , filed January 24, 1994, incoφorated herein by reference, the administration of BPI protein product to humans with endotoxin in circulation was described. [See also, von der Mδhlen et al., J. Infect. Dis. 772:144-151 (1995); von der Mδhlen et al., Blood 55:3437-3443 (1995); de Winter et al., /. Inflam. 45:193-206 (1995)]. In co-owned, co-pending U.S. Application Serial No. 08/644,287 filed May 10, 1995, the administration of BPI protein product to humans suffering from severe meningococcemia was described. In spite of treatment with antibiotics and state-of-the-art medical intensive care therapy, human mortality and morbidities associated with hemorrhage due to trauma remain significant and unresolved by current therapies. New therapeutic methods are needed that could reduce or ameliorate the adverse events and improve the clinical outcome of such patients. SUMMARY OF THE INVENTION The present invention provides novel methods for treating humans suffering from hemorrhage due to trauma, involving the administration of BPI protein products to provide clinically verifiable alleviation of the adverse effects of, or complications associated with, this disease state, including mortality and complications or morbidities. According to the invention, BPI protein products such as rBP^ j are administered to humans suffering from acute traumatic hemorrhage in amounts sufficient to reduce or prevent mortality and/or to reduce the incidence (i.e. , occurrence) or severity of complications or morbidities, including infection (e.g., surgical site infection) or organ dysfunction (e.g., disseminated intravascular coagulation, acute respiratory distress syndrome, acute renal failure, or hepatobiliary dysfunction). Also contemplated is use of a BPI protein product in the preparation of a medicament for the treatment of humans suffering from hemorrhage due to trauma. Numerous additional aspects and advantages of the invention will become apparent to those skilled in the art upon consideration of the following detailed description of the invention which describes presently preferred embodiments thereof. BRIEF DESCRIPTION OF THE FIGURES Fig. 1 shows the incidence of adverse events in rBPLj j and placebo treatment groups. DETAILED DESCRIPTION Acute hemorrhage due to trauma is a life-threatening condition with significant mortality and morbidities despite state-of-the-art medical intensive care. The administration of BPI protein products to humans suffering from hemorrhage due to acute traumatic injury (such as penetrating and/or blunt trauma) is expected to effectively decrease mortality and reduce the incidence (i.e. , occurrence) or severity of complications or morbidities associated with or resulting from hemorrhage due to trauma. Complications include infection (e.g. , in surgical sites, wounds, organs, anatomical spaces, the bloodstream, the urinary tract, or pneumonia) or organ dysfunction (e.g., disseminated intravascular coagulation, acute respiratory distress syndrome (ARDS), acute renal failure, or hepatobiliary dysfunction), and may include serious complications. An additional complication may be pulmonary dysfunction, which includes ARDS and pneumonia. These unexpected effects on the mortality and complications associated with and resulting from hemorrhage due to trauma indicate that BPI protein products effectively interfere with or block a number of the multiple poorly-understood pathophysiologic processes that have led to poor outcomes in this condition. BPI protein products may be used as adjunctive therapy in the treatment or prevention of organ dysfunction and serious infections. BPI protein products are expected to provide beneficial effects for patients suffering from hemorrhage due to trauma, such as reduced injury severity score, reduced length of time on ventilatory support and inotropic (vasoactive) therapy, reduced duration or severity of associated coagulopathy, reduced stay in the ICU, reduced stay in the hospital overall, and reduced incidence and duration of complications such as coagulopathy, respiratory failure, renal failure, hepatic failure, coma or altered mental state, adrenal cortical necrosis, and severe infection, including in wounds, organs, anatomical spaces, the bloodstream, the urinary tract, or pneumonia. Therapeutic compositions comprising BPI protein product may be administered systemically or topically. Systemic routes of administration include oral, intravenous, intramuscular or subcutaneous injection (including into a depot for long-term release), intraocular and retrobulbar, intrathecal, intraperitoneal (e.g. by intraperitoneal lavage), intrapulmonary using aerosolized or nebulized drug, or transdermal. The preferred route is intravenous administration. When given parenterally, BPI protein product compositions are generally injected in doses ranging from 1 μg/kg to 100 mg/kg per day, preferably at doses ranging from 0.1 mg/kg to 20 mg/kg per day, more preferably at doses ranging from 1 to 20 mg/kg/day and most preferably at doses ranging from 2 to 10 mg/kg/day. Treatment may be initiated immediately after the trauma or within a time period subsequent to the trauma (including, e.g. , within 6, 12 or 24 hours after trauma, or within a clinically reasonable time period determined by the treating physician, for example, 48 to 72 hours after trauma). Presently preferred is a continuous intravenous infusion of BPI protein product at a dose of 4 to 6 mg/kg/day, continuing for 48 to 72 hours. The treatment may continue by continuous infusion or intermittent injection or infusion, at the same, reduced or increased dose per day for, e.g. , 1 to 3 days, and additionally as determined by the treating physician. Alternatively, BPI protein products are administered intravenously by an initial bolus followed by a continuous infusion. One such regimen is a 1 to 20 mg/kg intravenous bolus of BPI protein product followed by intravenous infusion at a dose of 1 to 20 mg/kg/day, continuing for up to one week. Another such dosing regimen is a 2 to 10 mg/kg initial bolus followed by intravenous infusion at a dose of 2 to 10 mg/kg/day, continuing for up to 72 hours. Topical routes include administration in irrigation fluids for, e.g. , irrigation of wounds, or intrathoracic or intraperitoneal cavities. Other topical routes include administration in the form of salves, ophthalmic drops, ear drops, or medicated shampoos. For example, for topical administration in drop form, about 10 to 200 μL of a BPI protein product composition may be applied one or more times per day as determined by the treating physician. Those skilled in the art can readily optimize effective dosages and administration regimens for therapeutic compositions comprising BPI protein product, as determined by good medical practice and the clinical condition of the individual patient. As used herein, "BPI protein product" includes naturally and recombinantly produced BPI protein; natural, synthetic, and recombinant biologically active polypeptide fragments of BPI protein; biologically active polypeptide variants of BPI protein or fragments thereof, including hybrid fusion proteins and dimers; biologically active polypeptide analogs of BPI protein or fragments or variants thereof, including cysteine-substituted analogs; and BPI-derived peptides. The BPI protein products administered according to this invention may be generated and/or isolated by any means known in the art. U.S. Patent No. 5,198,541, the disclosure of which is incorporated herein by reference, discloses recombinant genes encoding and methods for expression of BPI proteins including recombinant BPI holoprotein, referred to as ΓBPIJQ (or rBPI) and recombinant fragments of BPI. Co-owned, copending U.S. Patent Application Ser. No. 07/885,501 and a continuation-in-part thereof, U.S. Patent Application Ser. No. 08/072,063 filed May 19, 1993 and corresponding PCT Application No. 93/04752 filed May 19, 1993, which are all incoφorated herein by reference, disclose novel methods for the purification of recombinant BPI protein products expressed in and secreted from genetically transformed mammalian host cells in culture and discloses how one may produce large quantities of recombinant BPI products suitable for incoφoration into stable, homogeneous pharmaceutical preparations. Biologically active fragments of BPI (BPI fragments) include biologically active molecules that have the same or similar amino acid sequence as a natural human BPI holoprotein, except that the fragment molecule lacks amino- terminal amino acids, internal amino acids, and/or carboxy-terminal amino acids of the holoprotein. Nonlimiting examples of such fragments include a N-terminal fragment of natural human BPI of approximately 25 kD, described in Ooi et al. , J. Exp. Med. , 774:649 (1991), and the recombinant expression product of DNA encoding N-terminal amino acids from 1 to about 193 or 199 of natural human BPI, described in Gazzano-Santoro et al. , Infect. Immun. 60:4754-4761 (1992), and referred to as rBPI^- j . In that publication, an expression vector was used as a source of DNA encoding a recombinant expression product (rBPI.23) having the 31 -residue signal sequence and the first 199 amino acids of the N-terminus of the mature human BPI, as set out in Figure 1 of Gray et al., supra, except that valine at position 151 is specified by GTG rather than GTC and residue 185 is glutamic acid (specified by GAG) rather than lysine (specified by AAG). Recombinant holoprotein (rBPIgg) has also been produced having the sequence (SEQ ID NOS: 1 and 2) set out in Figure 1 of Gray et al. , supra, with the exceptions noted for rBP^ and with the exception that residue 417 is alanine (specified by GCT) rather than valine (specified by GTT). Other examples include dimeric forms of BPI fragments, as described in co-owned and co-pending U.S . Patent Application Serial No. 08/212, 132, filed March 11 , 1994, and corresponding PCT Application No. PCT/US95/03125, the disclosures of which are incoφorated herein by reference. Preferred dimeric products include dimeric BPI protein products wherein the monomers are amino-terminal BPI fragments having the N-terminal residues from about 1 to 175 to about 1 to 199 of BPI holoprotein. A particularly preferred dimeric product is the dimeric form of the BPI fragment having N-terminal residues 1 through 193, designated rBPI^ dimer. Biologically active variants of BPI (BPI variants) include but are not limited to recombinant hybrid fusion proteins, comprising BPI holoprotein or biologically active fragment thereof and at least a portion of at least one other polypeptide, and dimeric forms of BPI variants. Examples of such hybrid fusion proteins and dimeric forms are described by Theofan et al. in co-owned, copending U.S. Patent Application Serial No. 07/885,911, and a continuation-in-part application thereof, U.S. Patent Application Serial No. 08/064,693 filed May 19, 1993 and corresponding PCT Application No. US93/04754 filed May 19, 1993, which are all incoφorated herein by reference and include hybrid fusion proteins comprising, at the amino-terminal end, a BPI protein or a biologically active fragment thereof and, at the carboxy-terminal end, at least one constant domain of an immunoglobulin heavy chain or allelic variant thereof. Similarly configured hybrid fusion proteins involving part or all Lipopolysaccharide Binding Protein (LBP) are also contemplated for use in the present invention. Biologically active analogs of BPI (BPI analogs) include but are not limited to BPI protein products wherein one or more amino acid residues have been replaced by a different amino acid. For example, co-owned, copending U.S. Patent Application Ser. No. 08/013,801 filed February 2, 1993 and corresponding PCT Application No. US94/01235 filed February 2, 1994, the disclosures of which are incorporated herein by reference, discloses polypeptide analogs of BPI and BPI fragments wherein a cysteine residue is replaced by a different amino acid. A preferred BPI protein product described by this application is the expression product of DNA encoding from amino acid 1 to approximately 193 or 199 of the N-terminal amino acids of BPI holoprotein, but wherein the cysteine at residue number 132 is substituted with alanine and is designated rBP^ j Δcys or rBP^ j . Other examples include dimeric forms of BPI analogs; e.g. co-owned and co-pending U.S. Patent Application Serial No. 08/212, 132 filed March 11, 1994, and corresponding PCT Application No. PCT/US95/03125, the disclosures of which are incoφorated herein by reference. Other BPI protein products useful according to the methods of the invention are peptides derived from or based on BPI produced by recombinant or synthetic means (BPI-derived peptides), such as those described in co-owned and co- pending U.S. Patent Application Serial No. 08/504,841 filed July 20, 1995 and in co- owned and copending PCT Application No. PCT/US94/ 10427 filed September 15, 1994, which corresponds to U.S. Patent Application Serial No. 08/306,473 filed September 15, 1994, and PCT Application No. US94/02465 filed March 11, 1994, which corresponds to U.S. Patent Application Serial No. 08/209,762, filed March 11 , 1994, which is a continuation-in-part of U.S. Patent Application Serial No. 08/183,222, filed January 14, 1994, which is a continuation-in-part of U.S. Patent Application Ser. No. 08/093,202 filed July 15, 1993 (for which the corresponding international application is PCT Application No. US94/02401 filed March 11, 1994), which is a continuation-iri-part of U.S. Patent Application Ser. No. 08/030,644 filed March 12, 1993, the disclosures of all of which are incorporated herein by reference. Presently preferred BPI protein products include recombinantly- produced N-terminal fragments of BPI, especially those having a molecular weight of approximately between 21 to 25 kD such as rBP^ or rBP^ j , or dimeric forms of these N-terminal fragments (e.g., rBPL^ dimer). Additionally, preferred BPI protein products include ΓBP^Q and BPI-derived peptides. Particularly preferred is rBPI 21 . The administration of BPI protein products is preferably accomplished with a pharmaceutical composition comprising a BPI protein product and a pharmaceutically acceptable diluent, adjuvant, or carrier. The BPI protein product may be administered without or in conjunction with known surfactants, other chemotherapeutic agents or additional known anti-microbial agents. One pharmaceutical composition containing BPI protein products (e.g., ΓBPLJQ, rBPJ^) comprises the BPI protein product at a concentration of 1 mg/ml in citrate buffered saline (5 or 20 mM citrate, 150 mM NaCl, pH 5.0) comprising 0.1 % by weight of poloxamer 188 (Pluronic F-68, BASF Wyandotte, Parsippany, NJ) and 0.002% by weight of poly sorbate 80 (Tween 80, ICI Americas Inc., Wilmington, DE). Another pharmaceutical composition containing BPI protein products (e.g., rBPLj j ) comprises the BPI protein product at a concentration of 2 mg/mL in 5 mM citrate, 150 mM NaCl, 0.2 % poloxamer 188 and 0.002 % polysorbate 80. Such combinations are described in co-owned, co-pending PCT Application No. US94/01239 filed February 2, 1994, which corresponds to U.S. Patent Application Ser. No. 08/190,869 filed February 2, 1994 and U.S. Patent Application Ser. No. 08/012,360 filed February 2, 1993, the disclosures of all of which are incoφorated herein by reference. Other aspects and advantages of the present invention will be understood upon consideration of the following illustrative examples. Example 1 addresses the effect of BPI protein product administration in humans on the mortality and complications associated with hemorrhage due to trauma. EXAMPLE 1 Clinical Study Protocol - Therapeutic Effects of BPI Protein Product A human clinical study was designed to examine the effect of an exemplary BPI protein product, rBP^ j , in the treatment of patients with acute hemorrhage due to trauma. Thus, a multicenter, randomized, double-blind, placebo- controlled trial was implemented comparing placebo treatment and rBPI 2 i treatment given over 48 hours in patients with acute hemorrhage due to trauma. Approximately 400 patients admitted to the emergency department with acute hemorrhage due to trauma and requiring transfusion of at least two units of blood were randomized in a 1 : 1 ratio for treatment with either rBP^ j or placebo. In addition to standard therapy, each patient received by continuous intravenous infusion either at 8 mg/kg over 48 hours (4 mg/kg/day x 2 days) or the equivalent volume of placebo. In most instances the weight of the patient in kilograms was determined as a best estimate. Efficacy was monitored from Day 1 to Day 15 by following patients for development of complications, such as impaired organ function and infection, and for survival. Safety was monitored by pre-treatment and serial post-treatment testing of chemistries and hematology parameters, as well as daily assessments for adverse events through Day 15. A final assessment of survival and adverse complications occurred on Day 29. Patients brought to the hospital with acute hemorrhage due to trauma were selected for enrollment in the study if they met the following inclusion and exclusion criteria. Inclusion criteria were: (1) age 18 (or age of consent) to 75 years, inclusive; (2) patient suffering from acute hemorrhage secondary to trauma; (3) study drug given within 6 or 12 hours of occurrence of the traumatic event (if precise time of event was unknown, best estimate was provided); (4) patient requires and has begun to receive a second unit of packed red blood cells; and (5) patient provides verbal informed consent or next of kin provides written informed consent. Exclusion criteria were: (1) a Triage Revised Trauma Score (TRTS, scale 0-12) less than 2.0 upon admission to the Emergency Department, see Table π below [Champion et al., Crit. Care Med. , 9(9):612-616 (1981); Greenfield et al. , Chapter 10, in Surgery Scientific Principles and Practices, J.B. Lippincott Co., Philadelphia, pp. 252-255 (1993)]; (2) severe head trauma (Glasgow Coma Score (GCS) _< 5 or equivalent evidence), see Table Ul below [Teasdale et al., Lancet, 1: 81 (1974)]; (3) isolated cranial injury; (4) spinal injury with paralysis; (5) burn injuries with at least 20% body surface area with second degree burns; (6) known positive HTV (test not mandatory at entry); (7) known pre-existing renal disease (creatinine > 2.0); (8) known pre-existing cardiac disease (NY Heart Association class greater than HI, see Table IV below [Braunwald, in Braunwald et al. , Heart Disease, The Textbook of Cardiovascular Medicine, 3rd ed. , W.B. Saunders Company, Philadelphia, PA, page 12 (1988); J. Am. Med. Ass'n, 249:539-544 (1988)]); (9) known pre-existing primary or metastatic malignancy in visceral organs; (10) arterial pH (at initial evaluation) < 6.8 or base deficit > 15 (if measured); (11) known current steroid therapy ( > 10 mg prednisone/day for > one month); (12) known pre-existing cirrhosis or active hepatitis; (13) pregnancy or lactation; (14) participation in other investigational drug studies (including investigational blood products) within previous 30 days; (15) weight (estimated) greater than 120 kg; and (16) a "do not resuscitate" (DNR) or equivalent order. Table π Triage Revised Trauma Score (TRTS)* ASSESSMENT METHOD CODING Respiratory Count respiratory rate in 15 10-29 4 Rate sec and multiply by 4 > 29 = 3 (RR) 6-9 2 1-5 1 0 0 Systolic Blood Measure systolic cuff pressure > 89 4 Pressure (SBP) in either arm by auscultation 76-89 3 or palpation 50-75 = 2 1-49 1 0 0 Glasgow Coma Calculate according to Table Convert GCS to Score HI below the Following Code: (GCS) 13-15 4 9-12 3 6-8 2 4-5 1 < 4 0 *The TRTS is the sum of the codes for RR, SBP and GCS (range 0-12). Table m Glasgow Coma Scale* Eye Opening Spontaneous 4 Response to sound 3 Response to pain 2 Never 1 Motor Response Obey commands 6 Localized pain 5 Normal flexion 4 (withdrawal) Abnormal flexion 3 (decorticate) No response 1 Verbal Response Oriented 5 Confused conversation 4 Inappropriate words 3 Incomprehensible sounds 2 None 1 * Scores range from i to 15 Table IV Modified New York Heart Association Functional Classification Class I. Patients with cardiac disease but with no limitation of physical activity. Ordinary physical activity causes no undue dyspnea, anginal pain, fatigue, or palpitation. Class US. Patients with slight limitation of physical activity. They are comfortable at rest and with moderate exertion. They experience symptoms only with the more strenuous grades of ordinary activity. Class JIM. Patients with moderate limitation of physical ability. They are comfortable at rest and with mild exertion. They experience symptoms with moderate grades of ordinary activity. Class HI. Patients with marked limitation of physical activity. They are comfortable at rest but experience symptoms even with the milder forms of ordinary activity. Class rv. Patients with inability to carry on any physical activity without discomfort. Symptoms of cardiac insufficiency or of the anginal syndrome may be present, even at rest, and are intensified by activity. The following were recorded for all patients randomized to treatment: (1) date and estimated time of incident, and date and time of admission to the Emergency Department; (2) for patients randomized and not treated, the reason for not treating; (3) from arrival at hospital until approximately 48 hours post- operatively, date, time, volume, and location that the patient received blood, blood products, and fluids such as packed red blood cells, whole blood, autotransfusion, platelets, fresh frozen plasma, crystaUoid, or colloid, at locations such as Emergency Department, Operating Room, Post-anesthesia Care Unit, or Surgical Intensive Care Unit; however, if the patient did not undergo surgery, the above items that were applicable were collected during study days 1 , 2, and 3; (4) date and time the second unit of blood was administered (which should have preceded surgery, to assure that hemorrhage is due to trauma, not surgery), and date and start and stop times of anesthesia; (5) date and start and stop times of surgery, estimated blood loss in operating room, and date and time in post-anesthesia care unit; (6) date and time study drug infusion began and ended, volume infused, and reasons for temporary or permanent discontinuation; if applicable, and if discontinued, quantity infused; (7) directed medical history (including extent and nature of injuries, intercurrent diseases, conditions contributing to bleeding, etc.), demographic and directed physical exam information, such as gender, age, weight (estimated or measured), height (estimated or measured), vital signs, physical signs of injury; (8) results of the pregnancy test performed during screening for eligibility of appropriate female patients (all women of child bearing potential, i.e. , all women who were not either surgically sterile or documented to be post-menopausal); and (9) results of the TRTS performed during screening for eligibility (including actual measurements). After transfusion of the second unit of blood was initiated, the investigator administered an unknown test drug from kits in numbered consecutive order. Each kit contained either was supplied as a clear, colorless, sterile non-pyrogenic solution in 10 mL single use glass vials at a concentration of 2 mg/mL in 5 mM sodium citrate/0.15 M sodium chloride buffer, pH 5.0 with 0.2 % poloxamer 188 and 0.002% polysorbate 80, containing no preservative. The rBPJ^ j was stored refrigerated at 2-8°C at all times prior to administration. The placebo was supplied as a clear, colorless sterile non-pyrogenic solution in 10 mL single use glass vials. It was composed of 0.2 mg/mL human serum albumin in 5 mM sodium citrate/0.15 M sodium chloride buffer, pH 5.0, containing no preservative. The placebo was also stored refrigerated at 2-8°C at all times prior to administration. The kit assigned to each patient contained a sufficient number of vials of study medication for all doses for that patient. Each vial contained 10 mL of test article. The study was administered to two groups ("active" rBP^ j and placebo control) as outlined above. The study medication was brought to room temperature prior to infusion. Throughout the dosing procedure, good aseptic technique for intravenous administration was followed. The study medication was administered by intravenous infusion into a central or peripheral vein over 48 hours. The infusion bag/tubing administration set was completely changed after 24 hours. Suitability of intravenous access was determined by easy withdrawal of blood from the access, as well as easy infusion of intravenous fluids without infiltration. The study medication was the sole agent administered in the chosen port during the course of the infusion protocol. The venous access port was not heparinized, but was flushed as necessary with physiologic saline. Any sign of a reaction at a site of infusion was recorded on the patient's case record form and source document as an adverse experience. Patients treated at selected study sites are assessed for: (1) blood levels of rBPI 2j . blood for the assessment of the rBPI 2j level is drawn at the following times (at selected study sites only): prior to the start of the infusion (up to 60 minutes prior to the start of the infusion), the following times (hours) after the start of the infusion; 1 , 4, 8, 12, 20, 24, 32, 36, 40, within 15 minutes prior to the completion of the 48 hour infusion, and the following times after completion of the infusion; 7 minutes (48:07), 15 minutes (48:15), 30 minutes (48:30), 1 hour (49:00), 3 hours (51:00), 6 hours (54:00), and 24 hours (72:00); (2) antibodies to rBPI 21 : blood for assessment of antibodies to rBPI 2| is drawn at selected study sites at the following times: Day 1 prior to study drug infusion, and Days 15 and 29, if the patient is still in hospital or returns to clinic (actual draw days may vary from Days 10-20 and Days 21-29); and (3) cytokines: blood for assessment of cytokines is drawn at selected study sites. The following safety laboratory panels were assessed at Day 1 prior to test drug infusion, Day 3 (after end of infusion) and Day 8, however, if patient is discharged on or prior to Day 8, assessment was made prior to discharge if possible: (1) hematology panel: hemoglobin, hematocrit, erythrocyte count, leukocyte count and differential, and platelet count; (2) serum chemistry panel: sodium, potassium, chloride, calcium, phosphorous, blood urea nitrogen, creatinine, uric acid, glucose (fasting), CPK, cholesterol, albumin, total protein, AST (SGOT), ALT (SGPT), bilirubin (total), GGT, LDH, and alakaline phosphatase. The following were recorded for all treated patients through Day 15 and/or Day 29 post-initiation of study drug infusion: (1) adverse events (continued through Day 29); (2) survival status including date and cause(s) of death (continued through Day 29); (3) dates in ICU (continued through Day 29); (4) dates in hospital (continued through Day 29); (5) dates on ventilator (continued through Day 29); (6) dates on dialysis or hemofiltration, specifying method (continued through Day 29); (7) concomitant medications, including daily amounts of blood transfused (continued through Day 15 or Day 29); (8) primary surgical procedures performed, for example, including re-operations but excluding procedures like placement of central lines, Swan-Ganz catheters, arterial lines; lumbar punctures, etc. (continued through Day 15); (9) injury severity score (ISS) based on diagnostic evaluations performed during current hospital stay; (10) daily assessment of organ dysfunctions and the presence of infections (continued through Day 15); (11) daily vital signs associated with and including daily maximum and daily minimum temperatures (continued through Day 15); and (12) inspection of infusion site used for study drug administration at least every eight hours, with observations documented in progress notes or the equivalent. Organ dysfunctions were assessed using the following definitions. The patient was considered to have disseminated intravascular coagulation (DIC) when there were: (1) abnormally low values for platelets (or there was a >25% decrease from a previously documented value) and either an elevated prothrombin time or an elevated partial thromboplastin time and clinical evidence of bleeding, or (2) if obtained, a confirmatory test was positive (FDP > 1 :40 or D-Dimers > 2.0). These abnormalities must have occurred in the absence of medically significant confounding factors such as liver failure, major hematoma, or anticoagulant therapy. The patient was considered to have acute respiratory distress syndrome (ARDS) when: bilateral pulmonary infiltrates consistent with pulmonary edema were present, and PaO 2 /FiO 2 < 200. These signs must have occurred in the absence of congestive heart failure or primary lung disease such as pulmonary embolus or pneumonia. The Pulmonary Artery Wedge Pressure (PAWP), when measured, must have been < 18 mm Hg. The patient was considered to have acute renal failure (ARF) when: (1) dialysis or hemofiltration was required (definition used for primary analysis), or (2) serum creatinine became abnormal with an increase of >_ 2.0 mg/dL in a patient with documented normal baseline creatinine, or (3) serum creatinine was >_ 3.0 mg/dL in a patient not known to have renal insufficiency, but whose (pretrauma) baseline creatinine was unknown, or (4) serum creatinine was doubled from admission or pre-rBPI 2j treatment level in a patient with previous renal insufficiency. These findings must not have been prerenal in nature (e.g. associated with dehydration or gastrointestinal bleeding) or due to rhabdomyolysis. Post-surgical hepatobiliary dysfunction (HBD) was evaluated only in patients without primary hepatic disease (e.g., hepatitis or cirrhosis), alcoholism, or biliary disease. The patient was considered to have hepatobiliary dysfunction when: the bilirubin exceeded 3.0 mg/dL, and either the alkaline phosphatase, gamma glutamyl transpeptidase (GGT), alanine aminotransferase (ALT, or SGPT) or aspartate aminotransferase (AST, or SGOT) exceeded twice the upper limit of normal. These findings must have occurred in the absence of confounding disease. Patients were also evaluated for infections in wounds, surgical sites (both superficial and deep incisional sites), organs, anatomical spaces, the bloodstream (bacteremia), the urinary tract, or the respiratory tract (pneumonia). The physician principal investigators were provided with the definitions of each organ dysfunction and were asked to record at pre-treatment and daily during Days 1-15 (a) whether each organ dysfunction was "Present", "Clinically present", "Not present or clinically present", or "Unknown" according to the definitions provided and (b) all available actual laboratory or clinical data required by the definition, whether or not the definition was met. Investigators were also provided with the definitions of infections and were asked to record (a) whether each type of infection was "Present" or "Not present" at any time during Days 1-15 and (b) if "Present", the actual culture or clinical data required by the definition. In order to provide a more objective analysis of these endpoints, and upon recommendation of an independent Data Safety Monitoring Board, computer programs were developed prior to the first efficacy interim analysis at 50% accrual to implement the same organ dysfunction and infection definitions using the actual laboratory, clinical and culture data required for each definition. The algorithmic approach defined each organ dysfunction as "Present," "Clinically present," "Not present or clinically present," or "Unknown" at pretreatment and daily during days 1-15. Each organ dysfunction was classified as "Unknown" on any given day if certain minimum assessments required by the definition were not provided on that day. The definition for "Present" on a given day required that all assessments had been made and that each assessment met its respective criterion for the specified organ dysfunction. Thus, patients for whom one or more required assessments were missing on a given day could not be classified as having met the definition for "Present" for that organ dysfunction for that day. On days on which assessments were incomplete, the organ dysfunction was classified as "Clinically present" if each of the nonmissing assessments met their respective criteria for "Present" for that organ dysfunction. Therefore, "Clinically present" implies that the organ dysfunction may have been present that day, based on incomplete evidence, and that no contradictory evidence was recorded that day. Since serious ARF was defined as the use of dialysis/hemofiltration on at least one day during Days 1-15, "Clinically present" was not applicable to serious ARF. An organ dysfunction was considered "Not present or clinically present" when none of the definitions for "Present, " "Clinically present" or "Unknown" were met for that day. Patients who were classified as having an organ dysfunction "Present" or "Clinically present" pretreatment were required to have satisfied the primary endpoint by another complication in order to have been classified as having met the primary endpoint during Days 1-15. For infections, the algorithmic approach defined each infection as "Present" or "Not present" during Days 1-15. Each definition for "Present" required that the definition be strictly met on at least one day during Days 1-15 according to the data provided. If the patient did not meet the "Present" definition of infection, they were classified as "Not present" for that infection. Serious complications were defined as the occurrence of the following serious infections: (1) a deep incisional surgical site infection, (2) an organ or anatomical space infection, (3) a secondary bloodstream infection, (4) a primary bloodstream infection, and (5) pneumonia; or the following serious organ dysfunctions: (1) disseminated intravascular coagulation (DIC) or coagulopathy, (2) acute respiratory distress syndrome (ARDS), (3) acute renal failure (ARF) requiring dialysis or hemofilt ration, and (4) hepatobiliary dysfunction (HBD). Patients were counted once as suffering from complications regardless of the number of complications. Across 19 sites, 1411 patients were screened and 401 patients were randomized into the study groups and received therapy. Among these 401 patients, 199 received placebo treatment and 202 received rBPI 2 j treatment. Thirty-one patients (15 placebo, 16 rBPI 2j ) did not receive the complete administration of study drug. Twelve patients (six placebo and six rBPI 2 j ) were withdrawn from the study before Day 29. Data from all patients who received any amount of study medication, even if infusion was incomplete, were included in all safety and efficacy analyses, whether or not the patient was withdrawn from the study. The mean age of the study population was 35 (range: 16-80 years); 80 % of the patients were under 45 years of age. The mean dosing weight was 79 kg (range: 45-145 kg). Seventy-seven percent of the patients were male. The traumatic injury source was classified as blunt trauma (50%), penetrating trauma (48%), or both (2%). Of the other trauma related characteristics, the mean TRTS was 10.6 (range: 0-12); the mean GCS was 13.2 (range: 3-15); the mean ISS (version '90) was 23.9 (range: 1-75), the mean number of PRBC units started prior to study drug infusion was 6.4 (range: 0-57), and the mean time from traumatic incident to drug infusion was 9.5 hours (range: 1.3-21.8 hours). There were no notable treatment group differences for age, weight, ethnicity, injury source, TRTS, GCS, and the time to infusion (p>0.10 controlling for site), but the placebo group had a somewhat higher proportion of females (ρ=0.11 , controlling for site). The number of units of packed red blood cells (PRBC) transfused prior to drug infusion was similar between patients randomized to rBPI 2 j and placebo. The mean ISS was slightly worse (p=0.07 controlling for site) for placebo patients (mean 25.1) than for rBPI 2j patients (mean 22.7). The prespecified primary efficacy analysis focused on the primary endpoint of mortality or serious complication (defined as serious organ dysfunction or serious infection as assessed by the algorithmic approach) occurring at any time after Hour 0 through Day 15. Overall mortality in this study was low (approximately 5-6%). Treatment groups were compared using Cox regression, stratifying by site and unadjusted for covariates. The results were analyzed by computer algorithm using the strict definitions of "Present" and also after incoφorating incomplete evidence (leading to classifications of "Present or Clinically present"). Regardless of the algorithmic method, the rate of mortality or serious complication by Day 15 was lower by 7% for rBPI 2j patients compared to placebo patients. The Kaplan- Meier estimates of event rates for mortality or serious complication using both algorithmic methods are shown below in Table V. TABLE V Placebo patients were approximately 1.27 times more likely to experience mortality or serious complication than rBPI 2 2 patients by both algorithmic methods as measured by the hazard ratio from Cox regression (rBPI 2 j to placebo hazard ratio = 0.79; p=0.13 for mortality or serious complication using "Present"; p=0.09 for mortality or serious complication using "Present or Clinically Present" , stratified by center). As a secondary analysis, the primary analysis was repeated with adjustment for significant covariates, resulting in the following hazard ratios: rBPI 2j to placebo hazard ratio using "Present" = 0.79, p=0.14 (adjusting for age, injury source, ISS'90 and units PRBC transfused prior to drug infusion); hazard ratio using "Present or Clinically Present" = 0.81 , p=0.16 (adjusting for age, ISS'90 and units PRBC transfused prior to drug infusion). Event incidence for each of the secondary efficacy measures assessed is shown in Fig. 1. Analysis of these secondary efficacy measures revealed lower frequencies of the following complications in patients treated with rBPI 2j compared to patients treated with the placebo preparation: any complication, any serious complication, any organ dysfunction, any serious organ dysfunction, any infection, any serious infection and pneumonia. Slight reductions were also noted in favor of ΓBPI 2 J treatment in the proportion of patients developing disseminated intravascular coagulation or coagulopathy, primary and secondary bloodstream infection and asymptomatic bacteriuria. Adverse events in this severely injured population were frequent in patients treated with either rBPI 2 j or placebo. There were, however, numerically higher percentages of patients with adverse events in the placebo group compared to the rBPI 2 j group. A higher percentage of patients were also noted to experience any extremely abnormal post-treatment laboratory result in the placebo treated group compared to the rBP^ j treated group. These data suggest a possible additional beneficial effect. In summary, this controlled clinical trial evaluating a single dosing regimen has demonstrated a trend in favor of rBPLji treatment in the primary endpoint of mortality or serious complication through Day 15. Reductions were also noted in the proportion of patients who experienced complications. These results, taken together, are consistent with a beneficial effect for treatment with rBP^ j in patients with hemorrhage due to trauma. Numerous modifications and variations of the above-described invention are expected to occur to those of skill in the art. Accordingly, only such limitations as appear in the appended claims should be placed thereon. SEQUENCE LISTING (1) GENERAL INFORMATION: (l) APPLICANTS: XOMA CORPORATION (ii) TITLE OF INVENTION: Therapeutic Uses of BPI Protein Products In Humans With Hemorrhage Due to Trauma (in) NUMBER OF SEQUENCES: 2 dv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Marshall, O'Toole, Gerstein, Murray & Borun (B) STREET: 6300 Sears Tower, 233 South Wacker Drive (C) CITY: Chicago (D) STATE: Illinois (E) COUNTRY: United States of America (F) ZIP: 60606-6402 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE. Patentln Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER. (B) FILING DATE: (C) CLASSIFICATION: (vn) PRIOR APPLICATION DATA. (A) APPLICATION NUMBER. 08/652,292 (B) FILING DATE: May 23, 1996 (C) CLASSIFICATION: (vm) ATTORNEY/AGENT INFORMATION: (A) NAME: Borun, Michael F. (B) REGISTRATION NUMBER: 25,447 (C) REFERENCE/DOCKET NUMBER: 27129/33959 PCT (ix) TELECOMMUNICATION INFORMATION (A) TELEPHONE : 312 /474 - 6300 (B) TELEFAX: 312/474-0448 (C) TELEX: 25-3Θ56 (2) INFORMATION FOR SEQ ID NO:l: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 31..1491 (ix) FEATURE: (A) NAME/KEY: mat_peptιde (B) LOCATION: 124..1491 (ix) FEATURE: (A) NAME/KEY: misc_feature (D) OTHER INFORMATION: "rBPI" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: CAGGCCTTGA GGTTTTGGCA GCTCTGGAGG ATG AGA GAG AAC ATG GCC AGG GGC 54 Met Arg Glu Asn Met Ala Arg Gly -31 -30 -25 CCT TGC AAC GCG CCG AGA TGG GTG TCC CTG ATG GTG CTC GTC GCC ATA 102 Pro Cyε Asn Ala Pro Arg Trp Val Ser Leu Met Val Leu Val Ala lie -20 -15 -10 GGC ACC GCC GTG ACA GCG GCC GTC AAC CCT GGC GTC GTG GTC AGG ATC 150 Gly Thr Ala Val Thr Ala Ala Val Asn Pro Gly Val Val Val Arg lie -5 1 5 TCC CAG AAG GGC CTG GAC TAC GCC AGC CAG CAG GGG ACG GCC GCT CTG 198 Ser Gin Lys Gly Leu Asp Tyr Ala Ser Gin Gin Gly Thr Ala Ala Leu 10 15 20 25 CAG AAG GAG CTG AAG AGG ATC AAG ATT CCT GAC TAC TCA GAC AGC TTT 246 Gin Lys Glu Leu Lys Arg lie Lys lie Pro Asp Tyr Ser Asp Ser Phe 30 35 40 AAG ATC AAG CAT CTT GGG AAG GGG CAT TAT AGC TTC TAC AGC ATG GAC 294 Lys lie Lys His Leu Gly Lys Gly His Tyr Ser Phe Tyr Ser Met Asp 45 50 55 ATC CGT GAA TTC CAG CTT CCC AGT TCC CAG ATA AGC ATG GTG CCC AAT 342 lie Arg Glu Phe Gin Leu Pro Ser Ser Gin He Ser Met Val Pro Asn 60 65 70 GTG GGC CTT AAG TTC TCC ATC AGC AAC GCC AAT ATC AAG ATC AGC GGG 390 Val Gly Leu Lys Phe Ser He Ser Asn Ala Asn He Lys He Ser Gly 75 80 85 AAA TGG AAG GCA CAA AAG AGA TTC TTA AAA ATG AGC GGC AAT TTT GAC 438 Lys Trp Lys Ala Gin Lys Arg Phe Leu Lys Met Ser Gly Asn Phe Asp 90 95 100 105 CTG AGC ATA GAA GGC ATG TCC ATT TCG GCT GAT CTG AAG CTG GGC AGT 486 Leu Ser He Glu Gly Met Ser He Ser Ala Asp Leu Lys Leu Gly Ser 110 115 120 AAC CCC ACG TCA GGC AAG CCC ACC ATC ACC TGC TCC AGC TGC AGC AGC 534 Asn Pro Thr Ser Gly Lys Pro Thr He Thr Cys Ser Ser Cys Ser Ser 125 130 135 CAC ATC AAC AGT GTC CAC GTG CAC ATC TCA AAG AGC AAA GTC GGG TGG 582 His He Asn Ser Val His Val His He Ser Lys Ser Lys Val Gly Trp 140 145 150 CTG ATC CAA CTC TTC CAC AAA AAA ATT GAG TCT GCG CTT CGA AAC AAG 630 Leu He Gin Leu Phe His Lys Lys He Glu Ser Ala Leu Arg Asn Lys 155 160 165 ATG AAC AGC CAG GTC TGC GAG AAA GTG ACC AAT TCT GTA TCC TCC AAG 678 Met Asn Ser Gin Val Cys Glu Lys Val Thr Asn Ser Val Ser Ser Lys 170 175 180 185 CTG CAA CCT TAT TTC CAG ACT CTG CCA GTA ATG ACC AAA ATA GAT TCT 726 Leu Gin Pro Tyr Phe Gin Thr Leu Pro Val Met Thr Lys He Asp Ser 190 195 200 GTG GCT GGA ATC AAC TAT GGT CTG GTG GCA CCT CCA GCA ACC ACG GCT 774 Val Ala Gly He Asn Tyr Gly Leu Val Ala Pro Pro Ala Thr Thr Ala 205 210 215 GAG ACC CTG GAT GTA CAG ATG AAG GGG GAG TTT TAC AGT GAG AAC CAC 822 Glu Thr Leu Asp Val Gin Met Lys Gly Glu Phe Tyr Ser Glu Asn His 220 225 230 CAC AAT CCA CCT CCC TTT GCT CCA CCA GTG ATG GAG TTT CCC GCT GCC 870 His Asn Pro Pro Pro Phe Ala Pro Pro Val Met Glu Phe Pro Ala Ala 235 240 245 CAT GAC CGC ATG GTA TAC CTG GGC CTC TCA GAC TAC TTC TTC AAC ACA 918 His Asp Arg Met Val Tyr Leu Gly Leu Ser Asp Tyr Phe Phe Asn Thr 250 255 260 265 GCC GGG CTT GTA TAC CAA GAG GCT GGG GTC TTG AAG ATG ACC CTT AGA 966 Ala Gly Leu Val Tyr Gin Glu Ala Gly Val Leu Lys Met Thr Leu Arg 270 275 280 GAT GAC ATG ATT CCA AAG GAG TCC AAA TTT CGA CTG ACA ACC AAG TTC 1014 Asp Asp Met He Pro Lys Glu Ser Lys Phe Arg Leu Thr Thr Lys Phe 285 290 295 TTT GGA ACC TTC CTA CCT GAG GTG GCC AAG AAG TTT CCC AAC ATG AAG 1062 Phe Gly Thr Phe Leu Pro Glu Val Ala Lys Lys Phe Pro Asn Met Lys 300 305 310 ATA CAG ATC CAT GTC TCA GCC TCC ACC CCG CCA CAC CTG TCT GTG CAG 1110 He Gin He His Val Ser Ala Ser Thr Pro Pro His Leu Ser Val Gin 315 320 325 CCC ACC GGC CTT ACC TTC TAC CCT GCC GTG GAT GTC CAG GCC TTT GCC 1158 Pro Thr Gly Leu Thr Phe Tyr Pro Ala Val Asp Val Gin Ala Phe Ala 330 335 340 345 GTC CTC CCC AAC TCC TCC CTG GCT TCC CTC TTC CTG ATT GGC ATG CAC 1206 Val Leu Pro Asn Ser Ser Leu Ala Ser Leu Phe Leu He Gly Met His 350 355 360 ACA ACT GGT TCC ATG GAG GTC AGC GCC GAG TCC AAC AGG CTT GTT GGA 1254 Thr Thr Gly Ser Met Glu Val Ser Ala Glu Ser Asn Arg Leu Val Gly 365 370 375 GAG CTC AAG CTG GAT AGG CTG CTC CTG GAA CTG AAG CAC TCA AAT ATT 1302 Glu Leu Lys Leu Asp Arg Leu Leu Leu Glu Leu Lys His Ser Asn He 380 385 390 GGC CCC TTC CCG GTT GAA TTG CTG CAG GAT ATC ATG AAC TAC ATT GTA 1350 Gly Pro Phe Pro Val Glu Leu Leu Gin Asp He Met Asn Tyr He Val 395 400 405 CCC ATT CTT GTG CTG CCC AGG GTT AAC GAG AAA CTA CAG AAA GGC TTC 1398 Pro He Leu Val Leu Pro Arg Val Asn Glu Lys Leu Gin Lys Gly Phe 410 415 420 425 CCT CTC CCG ACG CCG GCC AGA GTC CAG CTC TAC AAC GTA GTG CTT CAG 1446 Pro Leu Pro Thr Pro Ala Arg Val Gin Leu Tyr Asn Val Val Leu Gin 430 435 440 CCT CAC CAG AAC TTC CTG CTG TTC GGT GCA GAC GTT GTC TAT AAA 1491 Pro His Gin Asn Phe Leu Leu Phe Gly Ala Asp Val Val Tyr Lys 445 450 455 TGAAGGCACC AGGGGTGCCG GGGGCTGTCA GCCGCACCTG TTCCTGATGG GCTGTGGGGC 1551 ACCGGCTGCC TTTCCCCAGG GAATCCTCTC CAGATCTTAA CCAAGAGCCC CTTGCAAACT 1611 TCTTCGACTC AGATTCAGAA ATGATCTAAA CACGAGGAAA CATTATTCAT TGGAAAAGTG 1671 CATGGTGTGT ATTTTAGGGA TTATGAGCTT CTTTCAAGGG CTAAGGCTGC AGAGATATTT 1731 CCTCCAGGAA TCGTGTTTCA ATTGTAACCA AGAAATTTCC ATTTGTGCTT CATGAAAAAA 1791 AACTTCTGGT TTTTTTCATG TG 1813 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 487 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2 : Met Arg Glu Asn Met Ala Arg Gly Pro Cys Asn Ala Pro Arg Trp Val -31 -30 -25 -20 Ser Leu Met Val Leu Val Ala He Gly Thr Ala Val Thr Ala Ala Val -15 -10 -5 1 Asn Pro Gly Val Val Val Arg He Ser Gin Lys Gly Leu Asp Tyr Ala 5 10 15 Ser Gin Gin Gly Thr Ala Ala Leu Gin Lys Glu Leu Lys Arg He Lys 20 25 30 He Pro Asp Tyr Ser Asp Ser Phe Lys He Lys His Leu Gly Lys Gly 35 40 45 His Tyr Ser Phe Tyr Ser Met Asp He Arg Glu Phe Gin Leu Pro Ser 50 55 60 65 Ser Gin He Ser Met Val Pro Asn Val Gly Leu Lys Phe Ser He Ser 70 75 80 Asn Ala Asn He Lys He Ser Gly Lys Trp Lys Ala Gin Lys Arg Phe 85 90 95 Leu Lys Met Ser Gly Asn Phe Asp Leu Ser He Glu Gly Met Ser He 100 105 110 Ser Ala Asp Leu Lys Leu Gly Ser Asn Pro Thr Ser Gly Lys Pro Thr 115 120 125 He Thr Cys Ser Ser Cys Ser Ser His He Asn Ser Val His Val His 130 135 140 145 He Ser Lys Ser Lys Val Gly Trp Leu He Gin Leu Phe His Lys Lys 150 155 160 He Glu Ser Ala Leu Arg Asn Lys Met Asn Ser Gin Val Cys Glu Lys 165 170 175 Val Thr Asn Ser Val Ser Ser Lys Leu Gin Pro Tyr Phe Gin Thr Leu 180 185 190 Pro Val Met Thr Lys He Asp Ser Val Ala Gly He Asn Tyr Gly Leu 195 200 205 Val Ala Pro Pro Ala Thr Thr Ala Glu Thr Leu Asp Val Gin Met Lys 210 215 220 225 Gly Glu Phe Tyr Ser Glu Asn His His Asn Pro Pro Pro Phe Ala Pro 230 235 240 Pro Val Met Glu Phe Pro Ala Ala His Asp Arg Met Val Tyr Leu Gly 245 250 255 Leu Ser Asp Tyr Phe Phe Asn Thr Ala Gly Leu Val Tyr Gin Glu Ala 260 265 270 Gly Val Leu Lys Met Thr Leu Arg Asp Asp Met He Pro Lys Glu Ser 275 280 285 Lys Phe Arg Leu Thr Thr Lys Phe Phe Gly Thr Phe Leu Pro Glu Val 290 295 300 305 Ala Lys Lys Phe Pro Asn Met Lys He Gin He His Val Ser Ala Ser 310 315 320 Thr Pro Pro His Leu Ser Val Gin Pro Thr Gly Leu Thr Phe Tyr Pro 325 330 335 Ala Val Asp Val Gin Ala Phe Ala Val Leu Pro Asn Ser Ser Leu Ala 340 345 350 Ser Leu Phe Leu He Gly Met His Thr Thr Gly Ser Met Glu Val Ser 355 360 365 Ala Glu Ser Asn Arg Leu Val Gly Glu Leu Lys Leu Asp Arg Leu Leu 370 375 380 385 Leu Glu Leu Lys His Ser Asn He Gly Pro Phe Pro Val Glu Leu Leu 390 395 400 Gin Asp He Met Asn Tyr He Val Pro He Leu Val Leu Pro Arg Val 405 410 415 Asn Glu Lys Leu Gin Lys Gly Phe Pro Leu Pro Thr Pro Ala Arg Val 420 425 430 Gin Leu Tyr Asn Val Val Leu Gin Pro His Gin Asn Phe Leu Leu Phe 435 440 445 Gly Ala Asp Val Val Tyr Lys 450 455

Claims: WHAT IS CLAIMED IS: 1. A method of treating a human suffering from hemorrhage due to trauma comprising the step of administering a therapeutically effective amount of a bactericidal/permeability-increasing (BPI) protein product to said human. 2. The method of claim 1 wherein the BPI protein product is an amino-terminal fragment of BPI protein having a molecular weight of about 21 kD to 25 kD. 3. The method of claim 1 wherein the BPI protein product is rBP^ β or a dimeric form thereof. 4. The method of claim 1 wherein the BPI protein product is rBPI 21 . 5. Use of a BPI protein product in the preparation of a medicament for the treatment of humans suffering from hemorrhage due to trauma. 6. The use according to claim 5 wherein the BPI protein product is selected from the group consisting of amino-terminal fragments of BPI protein having a molecular weight of about 21 kD to 25 kD, rBPl23 or dimeric forms thereof, and rBP^ j .

Inventors: SCANNON PATRICK J, WEDEL NANCY, SCANNON, PATRICK, J., WEDEL, NANCY
Applicants: SCANNON PATRICK J, WEDEL NANCY, XOMA CORP, SCANNON, PATRICK, J., WEDEL, NANCY, XOMA CORPORATION
Application year: 1997
Main classification: A61K38
IPC codes: A61K31, A61K38, A61P7