INSECTICIDAL AND MITICIDAL COMPOSITIONS

Abstract: The invention relates to an insecticidal and miticidal composition which contains as active ingredients 4-bromo-2-(4-chlorophenyl)-1-(ethoxymethyl)-5-(trifluoromethyl)pyrrole-3-carbonitrile in combination with one or more compounds selected from the group consisting of benzoepin, nereistoxin-type insecticidal agents and diafenthiuron. The insecticidal and miticidal composition of the invention is effective against pests and mites having resistance to commercial insecticidal and miticidal agents.

Description: DESCRIPTION I SECTICIDAL AND MITICIDAL COMPOSITIONS FIELD OF THE INVENTION This invention relates to insecticidal and miticidal compositions which contain as active ingredi ¬ ents 4-bromo-2- (4-c I oropheny I ) - I - (ethoxy ethy I ) -5- (tr ifu loromethy I) pyrrole-3-carboni tr i le (hereinafter referred to as ch I orfenapyr) in combination with a second insecticidal and miticidal i ngredi ent (s) which can be effectively applied especially in the agro- hort i cu I tura I field. BACKGROUND OF THE I VENTION Ch lorfenapyr, which is an active ingredient of the insecticidal and miticidal composition of the inven ¬ tion, is known to be effective against insects such as Hemiptera pests such as leafhoppers {Do f tocepha l idae) , Lepidoptera pests such as diamond back moth (Plute l la xy loste I la) , common cutworm (Spodoptera I i tura) and apple leafminer (Phy I lonorycter r ingonie l la) and Thysanoptera pests such as Thr ips pa lmi and yellow tea thrips { Sp i rtothr ips dorsa l is) and agrohort i cu I tura I pests such as mites such as two-spotted spider mite ( Tetranychus urticae koch) , Kanzawa spider mite ( Tetra- nychus kanzawa i kishida) and Aculops pe lekass i. The second active ingredient of the insecti ¬ cidal and miticidal composition of the invention i n- eludes one or more of the following compounds: hexach I orohexahydromethanobenzod i oxath i ep i ne ox i de [benzoepin (endosu I fan) ] ; Nereistoxin-type insecticidal agents such as 1 , 3~b i s- (carbamoy I th i o) -2-(N, N-dimeth I- ami no) propane hydroch I or i de (cartap) , 5-d imethy I -ami no ~ 1 , 2, 3-tr i thiane oxalate (thiocyclam) and the like; and 1-tert-buty I -3- (2, 6—d i i sopropy l-4-phenoxypheny I) - thiourea (diafenthiuron), all of which are known to be effective agents against agricultural pests such as Hemiptera, Lepidoptera and Coleoptera insects. Although insecticidal and miticidal agents have been developed in order to control various pests such as agrohort i cu I tura I pests or hygienic pests and in practice have been used as a single or a mixed agent, pests which have acquired resistance against various agents have been appearing as a result of the repeated use of these agents. In particular, important economic pests in agrohort i culture such as Tetranychi dae, which have a propensity to easily develop resistance against pestici ¬ de I agents due to their ability to deposit large numbers of eggs and produce large numbers of generations which, themselves, require only a few days for development are of great concern. Resistance development in this pest family is also favored by a high mutation rate and frequent inbreeding, due to minimal migration. For these reasons, two-spotted spider mite ( Tetranychus urt icae koch) , Kanzawa spider mite ( Tetranychus kanzawa i ki sh i da) , Aculops pe lekass i , and the like have acquired resistance, to some degree, against almost all existing pesticidal agents. Therefore, in order to prevent and control the damage caused by Tetranyc i dae, development of a new insecticidal and miticidal agent which shows a high effect against Tetranych i dae which have acquired resistance against the conventional miticidal agents is h i gh I y des i rab le. However, to obtain an insecticidal and miti ¬ cidal composition which shows no cross-resistance with existing insecticidal and miticidal agents, has no toxicity problems and has little negative impact on the environment, is extremely difficult. Therefore, a means to delay or prevent the development of resistant strains of pest species is always being sought. In order to apply an effect i ve. agent as long as possible, a rota ¬ tional application of agents with different mechanisms of action is adopted for good pest management practice. However, this approach does not necessarily gi e satis- factory pest control. Therefore, after a resistance problem has occurred, a countermeasure to resistance by combining insecticidal and miticidal agents has been studied. However, a high synergistic action has not always been found. Therefore, it is an object of this invention to provide an insecticidal and miticidal composition which demonstrates a high controlling effect even against Tetranych i dae which have acquired resistance against ch I orfenapyr. SUMMARY OF THE INVENTION In order to establish a countermeasure to a resistance problem in Tetrachychi dae against chlor- fenapyr before such a problem occurs, the synergistic action with the existing insecticidal, miticidal and fungicidal agents was studied using resistant species which have been artificially established in the labora ¬ tory by selecting Tetrachych i dae which have been treated with ch I orfenapyr. Thus, it has now been found that an insecticidal and miticidal composition which contains as active ingredient chlorfenapyr in combination with one or more compounds selected from the group consisting of benzoepin, nereistoxin-type insecticidal agents and diafent iuron shows a joint action or synergistic effect which could not be foreseen from each individual ingre- d i ent a I one. DETAILED DESCRIPTION OF THE INVENTION Chlorfenapyr, which is an active ingredient of the insecticidal and miticidal composition of the inven ¬ tion, is a known compound (Japanese La id-open (Kokai) Patent Pub I i cat i on No. 104042/89). Compounds which are suitable for use as the second acti e ingredient in the composition of the invention such as one or more of the following compounds: 1) Benzoepin (6, 7, 8, 9, 10, 10-hexach loro- 1, 5, 5a, 9, 9a-hexahydro~6, 9-methano-2, 4, 3- benzodioxathiepi n ~ 3-oxi de) , 2) Nereistoxin-type insecticidal agents such as Cartap (1 , 3 ~ b i s (carbamoy I thi o) -2- (N, N- d i ethy la i no) propane hydroch I or i de) , Thi ocyc I am (5-d ime hy I ami no-1 , 2, 3- trithiane oxalate) and Bensultap (S, S' -2- di ethy I am i notr imethy I ene-d i (benzene- thi osu I fonate) , preferably cartap and thi ocyc I am, and 3) Diafenthiuron (1-tert. -buty I -3- (2, 6- d i i sopropy l-4-phenoxypheny I) thi ourea) (hereinafter referred to as Group A) are all known compounds and are commercial products which are readily ava i I ab I e. For the preparation of the insecticidal and miticidal composition of the invention, it is suitable to formulate as a wettable powder, aqueous concentrate, emulsion, liquid concentrate, sol (flowable agent), powder, aerosol, or the like, by conventional methods such as admixing chlorfenapyr and one or more compounds of Group A with a suitable carrier and aux i I I i a i es, such as emulsifiers, dispersants, stabilizers, suspend ¬ ing agents, penetrants, and the like. The content of the total active ingredients of the composition of the invention, expressed as weight/ weight %, is preferably in the range of about 1-90% for wettable powder, aqueous concentrate, emulsion, liquid concentrate and sol formulations. The preferable con- tent of total active ingredients is about 0.5-10% for powder formulations and about 0.01-2% for aerosol formu- I at ions. Carriers suitable for use in the insecticidal and miticidal compositions of the invention may be any solid or liquid carrier which is commonly used for an agrohort i cul tura I composition. Various surfactants, stabilizers and other auxiliary ingredients may be used according to the necessity. In commercially useful formulations, the composition of the invention may also be present in a mixture with other active agents, for example various insecticidal, miticidal, fungicidal and herbicidal agents, plant growth regulators, repel lants, attractants, synergists and fertilizers and fragrances, in order to expand its applicability. The ratio of chlorfenapyr to the compound(s) of Group A in the insecticidal and miticidal composition of the invention is about 1 wei ht part of chlorfenapyr to about 0.01-100 weight parts, preferably about 1-20 weight parts, of a compound (s) of Group A. The insecticidal and miticidal composition of the invention is particularly effective for the control of Tetranychi dae such as two-spotted spider mite ( Tetra ¬ nychus urticae koch) , Tetranychus c i nnabar i nus (bo i sduya I) , Kanzawa spider mite ( Tetranychus Kanzawa i k ish ida) , Tetran ichus viennens is zacher, and the like. Advantageously, the insecticidal and miticidal composition of the invention shows not only a synergis ¬ tic miticidal effect against the above-mentioned Tetranych i dae, but also demonstrates simultaneous con ¬ trol of troublesome pests such as leafroller moths ( Tortr ic idae) , Garpos in idae, leafminer moths (Lyoneti I ae) , plant bugs (Penta tomi dae) , aphids (Aphi didae) , leafhoppers (De I toe ipha I i dae) , thrips ( Thr i p i dae) , aphids (Aphi di dae) , diamond back moths (P lute 1 1 a xy loste l la) , Mamestra brass i cae, leaf beetles ( Chrysome I idae) , whiteflies (A I eyrod i dae) and the like on important agronomic crops such as fruit trees, for example citrus, apple and pear; tea plants; vegetables and the I i ke. Although the application amount may differ according to prevailing conditions such as the popula- tion density, the kinds and cultivation form of the target crop, the weather conditions, the manner of application, and the like, in general, the total amount of chlorfenapyr in combination with the compound (s) of Group A is about 0.1-1,000 g, preferably about 40-500 g, per 10 ares. In actual practice, the composition of the invention when in the form of a wettable powder, aqueous concentrate, emulsion, liquid concentrate, sol, or the like may be diluted with water and applied to the crop at an application rate of about 100-700 liters per 10 ares. When the inventive composition is formulated as a powder or aerosol, the crop may be treated with the undiluted formulation. The insecticidal and miticidal composition of the invention is further illustrated in the examples set forth hereinbelow. These examples are not intended to limit the scope of the invention. EXAMPLE 1 FORMULATION EXAMPLE 1 EMULSION Clorfenapyr 5 parts Benzoepin 40 parts Xylene 25 parts Dimethyl formamide 20 parts Sorpol 3005X 10 parts (Pol yoxyethy I ene type surfactant manufactured by Toho Chemical Industry Co., Ltd., commei— cial name) An emulsion is obtained by mixing ho oge- neously and dissolving the above-mentioned ingredients. EXAMPLE 2 FORMULATION EXAMPLE 2 WETTABLE POWDER Chlorfenapyr 5 parts Thiocycla 50 parts Carplex #80 10 parts (White carbon manufactured by Shionogi & Co., Ltd, commercial name) Zeekl i te SP 22 parts (Mixture of kaolinite and cericite anufac- tured by Zeekl ite Ind. , commercial name) Calcium I i gn i nsu Ifonate 8 parts A wettable powder is obtained by homogeneously mixing the above-mentioned ingredients by jet air mill. EXAMPLE 3 FORMULATION EXAMPLE 3 SOL (FLOWABLE AGENT) Chlorfenapyr 5 parts Diafenthiuron 25 parts Ethylene glycol 8 parts Sorpol AC3020 5 parts (Toho Chemical Ind. Co., Ltd., commercial name) Xanthan gum 0.1 parts Water 56.9 parts Chlorfenapyr, diafenthiuron and a previously prepared mixture of ethylene glycol, Sorpol AC3020 and xanthan gum are well mixed in water and dispersed. This slurry is then wet pulverized by Dyno ill (Shinmaru Enterprises) to obtain a sol (flowable agent). EXAMPLE 4 FORMULATION EXAMPLE 4 POWDER Chlorfenapyr 0.5 parts Cartap 3.5 parts White carbon 5.0 parts Clay 91 parts (Nippon Talc Co.. Ltd.) The above-mentioned ingredients are homoge ¬ neously mixed and pulverized to obtain a powder Each of the above-prepared formulations is suitable to be used as an agrochemi ca I . EXAMPLE 5 TEST EXAMPLE I In this experiment, the miticidal effect against female imagines (adults) of Kanzawa spider mite ( Te tranychus kanzawa i k ish ida) which are resistant to chlorfenapyr is evaluated. Round leaf disks (2 cm diameter) are cut out of a first leaf of kidney bean by a leaf punch and 4 sheets of the disks are placed on wet sanitary cotton in a plastic cup (8 cm diameter). On each leaf disk, 4 female imagines of Kanzawa spider mite ( Tetranychus kanzawa i kishida) which had acquired a strong resistance to chlorfenapyr are inoculated. After the inoculation, chlorfenapyr and a compound (s) selected from group A are dispersed in water containing 200 ppm of an extender (Sorpol 3005X) and di luted such that a predetermined concentration of active ingredient is obtained. Each plastic cup is sprayed with 3.5 ml of a test solution with a rotary spray tower (Mizuho Scientific Co., Ltd.) and stored in a constant temperature chamber held at 25 ° ±1°C (32 individuals are tested per concentration, 4-5 concentra ¬ tions are evaluated per formulation and 2 performances are repeated) . Two days after treatment, the number of living and dead female imagines of Kanzawa spider mite ( Tetranychus kanzawa i kishida) which had acquired a strong resistance to chlorfenapyr is counted and the mortality (%) is calculated according to the formula shown here inbe low. Mortality (%) = Number of dead mite x 100 Number of a I i ved mite + Number of dead mite Using these data, the LC 50 values are obtained by conventional probit analysis techniques. A co-toxi- city coefficient is calculated by applying Sun and Johnson's formula (J. Econ. Ent. , Vo I 53, p. 887, 1980) which is generally used to determine the degree of synergistic activity. The LC 50 value of each individual effective ingredient which constitutes the insecticidal and miti ¬ cidal composition of the invention is shown in Table I. The LC 50 values and the co-toxicity coefficients of the composition of the invention are shown in Table II. Co-toxicity coefficient = T c Actual toxicity index of mixture T c = x 100 Theoretical toxicity index of mixture For T c values greater than 100, the greater value indicates a stronger synergistic action. For a T° value equal to 100, an additive action is indicated. For T c values less than 100, the lesser value indicates a greater antagonistic action. A more detailed description of the calculation of the co-toxicity coefficient using the above-referenced Sun and Johnson formula fo I I ows. The LC 50 values of Test Compound A alone and Test Compound B alone and the LC 50 value of the (A + B) mixture M is determined. Actual toxicity index of mixture M = M Each LC 50 value of effective ingredient A and effective ingredient B and the LC 50 value of the mixture of A + B are used to determine the actual toxicity index as shown in the equation below. Theoretical toxicity index of mixture M = Th. M t i Th. M ' = (Toxicity index of A x %A in M Toxicity index of B x %B in M) Toxicity index of B = B t i , = LC 50 of A LC 50 of B Toxicity index of A = A* 1 A* '1 = 100 TABLE I Evaluation Of The Effect Of Test Compounds Against Female Imago Of Kanzawa Spider Mite Which Have Acquired Resistance Against Chlorfenapyr TEST COMPOUND LC 50 (ppm) Chlorfenapyr 1500 Be-zoepin 600 Cartap 2300 Thi ocyc I am 230 Diafenthiuron 540 By comparison, the LC 50 value for chlorfenapyr against a susceptible strain of spider mite is about 5 ppm. As can be seen from the data on Table I, the resistant strain of Kanzawa spider mite which was ob ~ tained by a long artificial selection procedure against chlorfenapyr in a laboratory on a colony of Kanzawa spider mite which had been collected in the field, has developed about a 300-fold resistance to chlorfenapyr. In the case of benzoepin, cartap, thiocyclam and diafenthiuron, this Kanzawa spider mite is thought to originate from a colony which had acquired resistance to these insecticidal agents prior to the time of col ¬ lection in the field. These compounds all showed low effects. TABLE Evaluation Of The Effect Of Test Mixtures Against Female Imago Of Kanzawa Spider Mite Which Have Acquired Resistance Against Chlorfenapyr LC 50 TEST MIXTURE RATIO 1 pom) T c Chlorfenapyr + Benzoepin 1 12 230 270 Chlorfenapyr + cartap 1 : 10 230 950 Chlorfenapyr + Thi ocyc I am 1 10 230 110 Chlorfenapyr + Diafenthiuron 1 1 I0 540 110 Chlorfenapyr : Second active Ingredient Co-ton i city Coefficient As can be seen from the data on Table M, the co-toxicity coefficient of the test mixtures is a value greater than 100, which is indicative of strong synei— gistic action.

Claims: CLA I MS 1. An insecticidal and miticidal composition which contains as active ingredients chlorfenapyr in combination with one or more compounds selected from the group consisting of benzoepin, nereistoxin-type insecti ¬ cidal agents and diafenthiuron. 2. The composition according to claim 1 wherein the active ingredients comprise chlorfenapyr in combina ¬ tion with one or more nereistoxin-type insecticidal agents. 3. The composition according to claim 2 wherein the nereistoxin-type insecticidal agent is cartap or thi ocyc I am. 4. The composition according to claim 1 wherein the active ingredients comprise chlorfenapyr in combina ¬ tion with benzoepin. 5. The composition according to claim 1 wherein the active ingredients comprise chlorfenapyr in combina ¬ tion with diafenthiuron. 6. The composition according to claim 1 wherein the chlorfenapyr is present in a ratio of about 1 weight part to about 0.01 - 100 total weight parts of one or more compounds selected from the group consisting of benzoepin, cartap, thiocyclam and diafenthiuron. 7. The composition according to claim 6 wherein the ratio is about 1 weight part of chlorfenapyr to about 1 - 20 weight parts of one or more compounds selected from the group consisting of benzoepin, cartap, thiocyclam and diafenthiuron. 8. A process for the preparation of a composition of claim 1 which comprises admixing the active ingredi ¬ ents with an agrohort i cu I tura I I y acceptable solid or I i qu i d car r i er. 9. The process according to claim 8 wherein the active ingredients comprise chlorfenapyr in combination with one or more compounds selected from the group consisting of benzoepin, cartap, thiocyclam and diafen- th i uron. 10. The process according to claim 9 wherein the chlorfenapyr is present in a ratio of about 1 weight part to about 1 - 20 weight parts of one or more compounds selected from the group consisting of benzoepin, cartap, thiocyclam and diafenthiuron.

Inventors: FUKUCHI TOSHIKI, FUKUCHI, TOSHIKI
Applicants: AMERICAN CYANAMID CO, FUKUCHI TOSHIKI, AMERICAN CYANAMID COMPANY, FUKUCHI, TOSHIKI
Application year: 1997
Main classification: A01N43
IPC codes: A01N43