Capillary electrophoresis

Abstract: A wide-bore capillary electrophoresis apparatus (200) for analysis of analyte ions is provided. The apparatus (200) has a wide-bore capillary (204) that has a restriction zone (106) at one or more ends (208, 216). The capillary has an inlet end (208) and an outlet end (216) and an opening (118) at each of said ends. The restriction zone (106) is capable of providing fluid communication between the wide bore (102) and the opening (118) at said end. The restriction zone (106) includes a narrow bore (114) extending to the opening and a transition zone (110) providing gradual change of bore diameter from the wide bore (102) to the narrow bore (114). The apparatus (200) further has a buffer source (212) to supply buffer to the inlet end (208) of the capillary (204) and a power supply (232) for supplying power to drive buffer and analyte ions through the capillary During CE, electrodes (224, 228) provide electrical communication between the power supply (232) and the inlet end (208) and the outlet end (216) to apply a voltage differential between said ends.

Description: The present invention is related to capillary electrophoresis, more particularly to capillary electrophoresis using a wide-bore capillary. Capillary electrophoresis (CE) has found wide acceptance in the analytical sciences, with application in diverse fields, including pharmaceutical analysis and bioscience research. Frequently CE has been called upon to provide semi-preparative quantities for further analysis, such as for microsequencing or mass spectrometry (MS) analysis. Bundled capillaries, multiple injections combined with fraction collection, and larger capillaries have all been used with some success to provide nanomolar quantities of analytes, but these approaches have for the most part resulted in reduced performance of the CE system. Clearly the need exists for capillary separation methods to more closely match the input loading requirements of the analytical techniques with which they are mated. An approach to increased loadability in CE is increasing the injected volume. Two methods could be used: (A) in a standard (e.g. 75 µm i.d.) column (i.e., capillary), increasing the amount injected into the column, thereby increasing the injection plug length; and (B) increasing the inside diameter (i.d.) of the column (e.g., to 150∼200 µm) to keep the injection plug length constant for an increased sample loading. However, studies have indicated rapid decreases in separation efficiency when either of these two approaches are attempted. EP-A-0,634,651 discloses a method for producing a capillary comprising the steps of providing first a capillary having a uniform inner width throughout its length, introducing then an etchant into the capillary, and producing a temperature gradient over the length of the capillary in such a way that a temperature-controlled increased expansion of the inner width of the capillary will take place during the etching process in the heated region thereof. A capillary produced in this way is suitable for use in an electrophoresis device. Analytical Chemistry, vol. 15, no. 4, February 1987, Washington DC, US, pages 6678-681, R.A. Wallingford: 'Characterisation of a microinjector for capillary electrophoresis', discloses a method of injecting sub-nanolitre samples into the bore of small bore capillaries having an inner diameter of 75µm in the separation zone. EP-A-0,459,241 discloses apparatus for concentrating a solute sample which can be subsequently released for analysis by capillary electrophoresis. The apparatus comprises a tube containing a short length of packed particles or gel adapted to retain sample solutes which particles are retained in the tube by tube constrictions or porous plugs. The tube includes an inlet for introducing a sample into the tube and an outlet for introducing the retained sample into a capillary electrophoresis tube. The present invention seeks to provide improved electrophoresis. According to an aspect of the present invention there is provided wide-bore capillary electrophoresis apparatus as specified in claim 1. According to another aspect of the present invention there is provided a method of capillary electrophoresis analysis as specified in claim 9. According to another aspect of the present invention there is provided a method of making wide-bore capillary electrophoresis apparatus as specified in claim 10. An embodiment of the present invention is described below, by way of example only, with reference to the accompanying drawings, in which: Fig.1 shows the result of CE separation of rhGH tryptic digest with a standard 75 µm i.d. capillary compared to a preferred embodiment of 180 µm i.d. capillary. Fig.2A shows the result of CE separation of rhGH tryptic digest with a standard 75 µm i.d. capillary with a 13 nL injection volume. Fig.2B shows the result of CE separation of rhGH tryptic digest with a standard 75 µm i.d. capillary with a 46 nL injection volume. Fig.2C shows the result of CE separation of rhGH tryptic digest with a 69 nL injection volume to a preferred embodiment of 180 µm i.d. capillary. Fig.2D shows the result of CE separation of rhGH tryptic digest with a 138 nL injection volume to a preferred embodiment of 180 µm i.d. capillary. Fig.3 shows the graphical representation of plate height as a function of velocity with different amounts of siphoning pressure, determined theoretically for a 180 µm i.d. straight (unrestricted) capillary and showing data point a,b,c for a restricted 180 µm i.d. capillary. Fig.4 shows a sectional view (along the axis) of an embodiment of restricted capillary. Fig.5 shows the result of CE separation of rhGH tryptic digest with the preferred 180 µm i.d. capillary at different pressure heads. Fig.6 shows a graphical representation of the temperature profile inside a 75 µm i.d. capillary compared to that inside a 180 µm i.d. capillary. Fig.7 shows a graphical representation of the plate height as a function of the radial (axis to wall) temperature difference for a 75 µm i.d. capillary and for a 180 µm i.d. capillary. Fig.8 shows the result of CE separation of rhGH tryptic digest with a preferred embodiment of 180 µm i.d. capillary at 25°C with a standard 75 µm i.d. capillary at 25°C, 35°C, and 45°C. Fig.9 shows the result of CE separation of rtPA tryptic digest and fraction collection using a preferred 180 µm i.d. capillary. Fig.10 shows the mass spectrum of MALDI-TOF mass spectrometry of the collected fraction 9A of Fig.9. Fig.11 shows an isometric view of an embodiment of CE apparatus. We have found that by restricting the end portion(s) of the bore (i.e., lumen) of a wide-bore capillary, siphoning can be substantially avoided, thereby enabling the application of wide-bore capillaries, i.e., capillaries with a bore larger than the standard bore of about 75 µm i.d., for CE analysis. Several mechanisms by which band broadening may quickly increase in wide-bore capillaries were investigated: (1) siphoning between electrode reservoirs; (2) temperature effects due to Joule heat, including both radial temperature profile and average buffer temperature; and (3) pH changes within the buffer reservoirs due to the increased electrical current in a wide bore. This disclosure considers these mechanisms individually in wide-bore capillaries, and provides practical solutions which can result in successful analyte separations on such capillaries. Our results demonstrate (as shown in Fig. 1) that with appropriate attention to these factors good system performance can be obtained, and that the increased loadability of wide-bore capillaries permits much more practical injection volumes when fraction collection is required. In Fig. 1, the bottom curve 1B shows the CE result using a standard 75 µm i.d. capillary and the top curve 1A shows the CE result using a preferred wide-bore (180 µm i.d.) capillary. The effective length of each capillary is 41.5 cm and the total length of each capillary is 50 cm. In the wide-bore capillary, each end is restricted with a 50 µm i.d. restrictor. The two curves shows comparable separation efficiency. This indicates that the presence of restrictors results in good CE performance even when a wide-bore capillary is used. The run parameters are described in the following: Buffer: 100 mM phosphate, pH 2.0. Injection: pressure. Detection: UV 200 nm. High voltage: constant current mode 52 µA and 7.0 kV for 75 µm i.d. capillary, 300 µA and 7.4 kV for 180 µm i.d. capillary. CE instrument: HP1601A HP 3D CE. Separation Efficiency for Large Sample Volumes Although it is possible to inject greater volumes into standard diameter (e.g., 75 µm i.d.) CE capillaries for increased sample loading, this can only be used to a very limited degree. The increased injection volume results in an increased injection plug variance, which would soon become the dominating plate height (i.e., the theoretical plate height, hereinafter referred to as "plate height") contributor. With increased injection volume, separation efficiency will suffer because the limiting efficiency of capillary systems is determined by the ratio of the injected volume to the analytical volume of the system. Figs. 2A-2D show the effect plug length and capillary i.d. have on the CE separation efficiency. Fig. 2 (A, B) illustrates the problem of increased i.d.: in a 75 µm i.d. capillary a 46 nL injection volume (10 mm injection plug length) will effectively destroy the separation (Fig 2B) whereas a 13 nL injection volume gives acceptable result (Fig.2A). However, with the restricted capillaries, increased inside diameter (i.d.) of the capillary can produce good CE separation, since the injected volume increases as the square of the diameter and the increased injection volume can be accommodated with no increase in injection plug length. For example, an increase in capillary diameter, from 75 to 180 µm. would increase the injected volume by a factor of about 6 with no change in injection plug length and therefore with no change in separation efficiency (comparing Fig.2A with Fig.2C, which involves a 180 µm i.d. capillary with a 69 nL injection volume). With the restricted 180 µm i.d. capillary, injection volumes of up to 138 nL (5.4 mm plug length) have been used, with only minimal degradation of separation efficiency (Fig.2D). 1. Prevention of Siphoning in Wide-Bore Capillaries When using wide-bore capillaries, the wider bore, i.e., larger bore, reduces the flow resistance and, as a result, the flow through the capillary due to siphoning can be significant. Because the flow is pressure-driven, siphoning can cause large changes in velocity and can increase band broadening. This effect was examined theoretically and experimentally. It was found that by modifying the capillary with flow restrictors, siphoning effects were greatly reduced. In a standard capillary, i.e., nonrestricted capillary normally used in the art, the change in velocity will vary linearly with the pressure head, and with the square of the diameter (see, e.g., Keely, C. A; van de Goor, T. A. and McManigill, D., Anal. Chem., 66(1994), 4236-4242; Aris, R., Proc. R. Soc. London, A235(1956), 66-77; Datta, R. and Kotamarthi, V. R., AICHE J., 36(1990), 916-926). Δν( r,p )= pr 2 1 8η L (1-2 r 2 ) Eq. 1 The plate height function is more complicated, but in wider-bore capillaries, plate height will increase linearly with pressure, and with the fourth power of the diameter. H = 2 û K d Eq. 2 where In the equations in this disclosure, the variables are: D = diffusion coefficient H = plate height J 0 , J 1 = Bessel function of first kind, order 0 and 1 K d = total dispersion k 1 , k 2 = thermal conductivity of buffer and fused silica L = total length of capillary p = pressure r = normalized radius variable r', r" = integration variables representing r r i , r 0 = capillary radius, inside and outside T 0 = temperature outside capillary ΔT = temperature difference, center to wall u = average bulk velocity Δv = change in velocity function w 0 = power dissipated α = temperature coefficient of resistivity η = viscosity of buffer µ = -α Thus, siphoning can severely impact the separation efficiency in wide-bore capillaries. Fig. 3 gives the plate height as a function of velocity for a 50 cm straight 180 µm i.d. capillary, assuming that only diffusion and the pressure flow profile contribute to the plate height. The three curves represent different amounts of siphoning via pressure heads (inlet above outlet): 0 mm (shown by the bottom curve 3A), 2 mm (0.2 mbar, middle curve 3B), and 4 mm (0.4 mbar, shown by the top curve 3C). As expected, even a small pressure head causes enough siphoning to greatly increase the plate height in this column By comparison, the pressure head would have to be 30 mm (3 mbar) in a 75 µm i d capillary to generate the plate heights given in the 0.2 mbar curve (middle curve, 3B). Data points a, b, and c are for a preferred capillary corresponding to the three siphoning pressures and will be described infra. To make a capillary that has little siphoning (i.e. reduced siphoning compared to a capillary with the same capillary i.d.), the ends of the wide-bore capillaries are heated (e.g. by a flame) to soften the capillary such that the ends gradually collapse to narrow the bore to a desired geometry with a slender narrow bore (or restriction) proximate the very end of the capillary. In the restriction, the bore gradually increases in diameter (such as through a transition portion) to the size of the full wide-bore of the capillary. This heating and bore-narrowing procedure is referred to as "flame restricting" herein. Typically, an end of the capillary is heated in a relatively uniform fashion such that the restriction can be formed with a generally straight centerline (or axis) with circular cross section. For example, during flame-restricting, the end portion of the capillary can be heated and rotated in a flame while its geometry is being monitored in a microscope. It is contemplated that the end portion can also be held and drawn to facilitate the formation of the narrow bore. Automated equipment can be used to make such restrictions on the ends of capillaries. An embodiment of a capillary is shown in Fig. 4. The capillary has a wide-bore 102. The capillary 100 also has at least one end 104 that has a restriction 106 to reduce siphoning during CE. The restriction 106 has a transition zone 110 that gradually narrows to a slender narrow-bore 114 that extends for a length to an opening 118 that provides fluid communication with the outside. Preferably, the capillary has a restriction on each end of the capillary. The capillary has a wide bore (i.e., with an i.d. equal to or larger than about 75 µm), which preferably has an i.d. of about 100 to 500 µm, more preferably about 100 to 250 µm, even more preferably about 180 to 200 µm. The size of the restriction is selected to reduce siphoning to an acceptable level. As an example, for a capillary with about 50 cm total length, about 40 cm effective length, and a CE voltage of about 7 kV, the short restriction formed can be about 10 to 1000 µm long and about 20 to 75 µm i.d., preferably about 250-750 µm long and 20 to 50 µm i.d., more preferably about 0.5 mm long with an i.d. of 50 µm. The selection of the length and i.d. of the restriction depends on factors such as the length and i.d. of the capillary, as well as the voltage applied for CE. A person skilled in the art will be able to select these parameters based on the present disclosure. It is understood that although the restriction is preferably integrally connected to the wide-bore part of the capillary (as in flame-restriction the wide-bore capillary), the restriction can be a separate piece connected to the wide-bore portion by a fitting, by adhesion, as a plug, and the like. The restricted capillary can be made with standard materials that are used for making CE capillaries, for example, nonmetallic inorganic material (e.g., fused silica), polymeric material such as polytetrafluoroethylene (PTFE), polyether ether ketone (PEEK), and-the like. Capillaries of the above sizes and shapes can reduce the effects of siphoning to an acceptable amount. For example, the 180 µm i.d. capillary with end restrictions of about 0.5 mm long with an i.d. of 50 µm is comparable to a 135 µm i.d. capillary in separation efficiency. A further technique to reduce siphoning is to balance the levels of the buffer reservoirs in which the electrodes are located. The buffer replenishment feature of the HP 3D CE apparatus permits such buffer leveling of the reservoirs, which, together with the modified capillaries, results in improved reproducibility of the migration time and peak area, as well as better control of pressure injections and fraction collection Fig. 5 demonstrates the separation of analytes in a 180 µm restricted capillary (having 0.5 mm long restriction with an i.d. of 50 µm) with imposed pressure head of 0 mm (associated with peak a), 2 mm (associated with peak b), and 4 mm (associated with peak c). The velocity and plate heights calculated from the indicated peaks are plotted in Fig. 3. From Fig 3, it can be seen that the theoretical data points a, b, and c, which correspond to the peaks a, b, and c for a restricted capillary in Fig. 5, are indeed much lower than those in the theoretical curves, i.e., curves 3A, 3B, and 3C, for the non-restricted capillary. In fact, CE analysis with parameters used in the runs of Fig. 5 could not be done using a straight bore capillary of 180 µm, due to the siphoning effects, as well as large changes in migration time. 2. Temperature Effects A concern for CE analysis using a wide-bore capillary is the detrimental effect (band broadening) on separation caused by nonuniform temperature in the capillary. For a given applied CE voltage, the electrical current through the capillary increases with the square of the diameter, thus wide-bore capillaries of average length (as in conventional small-bore capillaries) in CE require a larger electrical current, which leads to a large power dissipation in the capillary. This power is dissipated as Joule heating, and leads to a radial temperature gradient and an increased average temperature. We have found that using a restricted wide-bore capillary to analyze peptide sized molecules the actual increase in plate height is negligible. (a) Radial Temperature Profile The radial temperature gradient is a function of the power dissipated, capillary dimensions, thermal characteristics of the buffer and capillary, and the external temperature. This radial temperature gradient can be calculated (Brown, J F. and Hinckley, J, O, N, J Chromatogr., 109(1975), 218-224). with β= µ w 0 k 1 ; µ=-α Eq.3b Fig. 6 shows the thermal profiles calculated for 180 µm i.d (curve 6A) and 75 µm i.d. (curve 6B) capillaries with the external temperature at 45°C. Although the 180 µm gradient is much larger than that of the 75 µm, there is still only 1.2°C difference between the temperature of the outside wall and that at the center. The resultant plate height can be calculated by assuming the temperature profile causes a viscosity profile, which in turn causes a parabolic velocity profile (Eq. 2, 4) Δν ( r,ΔT )= µΔ T û 2 (1-2 r 2 ) Eq .4 Fig. 7 shows the theoretical plate height as a function of the difference in temperature between the wall and the center, assuming the velocity changes 2% per °C and assuming a diffusion coefficient representative of peptides. As shown in Fig. 7, although the effect on plate height is greater for a 180 µm capillary (curve 7A) than for a 75 µm capillary (curve 7B), it is still less than 0.1 µm for the predicted temperature difference of 1.2°C. This effect (i.e., contribution to increase plate height by temperature difference) will be negligible, especially when the contribution to plate height due to the finite injection volume is taken into account. In a system which has siphoning in addition to a temperature profile, the increase in plate height will be greater than the sum of the two plate height effects because the profiles will interact with each other. This is why reducing siphoning is so important in reducing band broadening. (b) Average Buffer Temperature Because CE is effected by an electrical potential difference applied to the two ends of the capillary, heat is dissipated in the capillary. For example, three watts of energy dissipated in a 375 µm o.d. 180 µm i.d. capillary will raise the average temperature inside the capillary by about 20°C over the control temperature when forced air cooling is used. Furthermore, mounting and sealing points inaccessible to cooling may form hot spots where the buffer may boil. Fixture designs that allow proper cooling can minimize hot spots. We have found that for a preferred form of capillary, the average temperature does not significantly affect the CE separation. Fig.8 shows the results of analyte separations done using a restricted 180 µm i.d. capillary with the control temperature held at 25°C and also in a 75 µm i.d. capillary with control temperatures of 25°C, 35°C and 45°C. Comparison of the velocities and efficiencies indicates that the inside of the 180 µm, 25°C capillary is approximately the same temperature as inside the 75 µm, 45°C capillary. There is no significant difference in band broadening between the four runs, indicating that the average temperature increase is not a problem for this separation. This figure also supports the theoretical prediction that the temperature gradient effect on plate height is negligible. 3. Buffer Depletion due to Coulombic Titration Because up to 300 µA current is often applied for wide-bore capillary electrophoresis, buffer depletion due to coulombic titration occurs much faster in such wide-bore capillaries than in standard capillaries. For example, Table 1 shows the pH change of 100 mM pH 2.5 phosphate buffer during electrophoresis for given volumes of buffer when 300 µA current is applied. To reduce buffer depletion, 4-5 mL triple vials (i.e., vials three-times the size of normal vials) were used. Using these vials, a two hour run at 300 µA resulted in a buffer pH change of less than 0.1 pH units. 30.0min 60.0min 90.0min 120.0min 0.5ml 0.198 0.420 0.709 1.268 1.0ml 0.098 0.198 0.304 0.420 2.0ml 0.049 0.098 0.148 0.198 4.0ml 0.024 0.049 0.073 0.098 Applications The capillaries and CE apparatus can be used for analyzing analytes as in using a conventional CE apparatus. The large sample volume injections allow complex samples to be analyzed with adequate material in the individual peaks to permit subsequent analyses, without sacrificing resolution. As an example, tryptic digest of rtPA was analyzed with a modified 180 µm i.d. capillary (Fig. 9). The fraction marked as 9A (shaded) was collected from the capillary using 25 mbar pressure for 12 seconds immediately after the peak was detected. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can be used to analyze the fractions collected. Such an analysis identified this fraction as a glycopeptide fragment (T45) of the rtPA digest (Fig.10). The CE columns (i.e., capillaries of the present invention), due to their increased loadability, are more suitable than standard capillaries to permit a complimentary match between CE and emerging micro LC systems, interfacing techniques for hyphenated instruments, and semi-preparative sample production. This in turn would facilitate the construction of a unified apparatus for micro separations work of high sensitivity and separation efficiency. To use a restricted capillary, standard equipment for CE can be adapted for use with a wide-bore application. Such CE equipment and methods are known in the art. An example of CE equipment that can be adapted for CE using a wide-bore capillary is HP1601A HP 3D CE (Hewlett-Packard Co., Palo Alto, CA). A preferred embodiment of CE equipment is illustrated in Fig. 11. In the CE apparatus 20 shown in Fig. 11, a wide-bore capillary 204 has one end (e.g., inlet end) 208 immersed in a buffer in a first container (e.g buffer supply) 212 and another end (e.g., outlet end) 216 immersed in a buffer in a second container (e.g., collection bottle) 220. A first electrode 224 is immersed in the buffer in the first container 212 and a second electrode 228 is immersed in die buffer in the second container 220 to provide a voltage differential for driving CE. The first electrode 224 and the second electrode 228 are electrically connected to a voltage supply 232 that provides the power to drive CE.

Claims: A wide-bore capillary electrophoresis (CE) apparatus (200) for analysis of analyte ions, comprising: (a) wide-bore capillary (204) having an inlet end (208) and an outlet end (216), a wide bore zone having a wide bore (102) between said ends, and an opening (118) at each of said ends, at least one of said ends (118) having a restriction zone (106) capable of providing fluid communication between the wide bore (102) and the opening (118) at said end, the restriction zone (106) including a narrow bore (114) extending to the opening and including a transition zone (110) providing gradual change of bore diameter from the wide bore (102) to the narrow bore (114), the length of the wide bore (102) extending for almost the whole length of the capillary; (b) a buffer source (212) to supply buffer to the inlet end (208) of the capillary (204); and (c) power supply (232) for supplying power to drive buffer and analyte ions through the capillary (204), including electrodes (224, 228) in electrical communication with the inlet end (208) and the outlet end (216) to provide voltage differential between said ends during CE. The apparatus according to claim 1 wherein the wide bore capillary (204) is an integral, unitary piece. The apparatus according to any of claims 1 to 2 wherein each of the ends (208, 216) of the wide bore capillary (204) has a restriction zone (106). The apparatus according to any of claims 1 to 3 wherein the wide bore (102) has an inside diameter of 75 µm or larger. The apparatus according to any of claims 1 to 4 wherein the wide bore (102) has an inside diameter of 100 µm to 500 µm. The apparatus according to any of claims 1 to 5 wherein the narrow bore (114) has an inside diameter of 20 µm to 75 µm. The apparatus according to any of claims 1 to 6 wherein the narrow bore (114) has an inside diameter of 20 µm to 50 µm. The apparatus according to any of claims 1 to 7 wherein the narrow bore (114) has a length of 10 µm to 1000 µm. A method for capillary electrophoresis (CE) analysis of analytes, comprising: applying a voltage differential between an inlet end (208) and an outlet (216) of a wide-bore capillary (204) to drive a buffer and analyte ions through the capillary, the wide-bore capillary (204) having an opening (118) at each of said ends (208, 216) and a wide bore (102), at least one of said ends (208, 216) having a restriction zone (106) capable of providing fluid communication between the wide bore (102) and the opening at said end, the restriction zone (106) including a narrow bore (114) extending to the opening (118) and including a transition zone (110) providing gradual change of bore diameter from the wide bore (102) to the narrow bore (114), such that the restriction zone (106) substantially prevents siphoning, wherein the length of the wide bore (102) zone extends for almost the whole length of the capillary. A method of making a wide-bore capillary electrophoresis (CE) apparatus (200) for analysis of analyte ions, comprising: (a) making a wide-bore capillary (204) by providing a narrowing of the bore of at least one end portion of a capillary with a wide-bore zone and an inlet end and an outlet end to form a restriction zone (106) which is capable of providing fluid communication between the wide bore zone (102) and the opening (118) at said end, the restriction zone (106) including a narrow bore (114) extending to the opening (118) and a transition zone (110) providing gradual change of bore diameter from the wide bore zone (102) to the narrow bore (114), wherein the wide-bore zone extends for almost the whole length of the capillary; (b) providing a container (212) for containing a buffer to supply buffer to the inlet end (208) of the capillary (204) during capillary electrophoresis (CE); and (c) connecting electrodes (224, 228) to a power supply (232) for supplying power to drive buffer and analyte ions through the capillary (204) during CE, one of said electrodes (224, 228) being positioned in said container (212) to provide electrical communication via the buffer with the inlet end (208) of the capillary during CE, one electrode (228) being positioned proximate said outlet end (216) of the capillary (204) to provide electrical communication thereto during CE such that a voltage differential is applied between said ends (208, 216) of the capillary (204).

Inventors: HOLLOWAY ROBERT R, KEELY-TEMPLIN CATHERINE A, MCMANIGILL DOUGLASS, YIN HONGFENG, HOLLOWAY, ROBERT R., KEELY-TEMPLIN, CATHERINE A., MCMANIGILL, DOUGLASS, YIN, HONGFENG
Applicants: AGILENT TECHNOLOGIES INC, AGILENT TECHNOLOGIES, INC. (A DELAWARE CORPORATION)
Application year: 1997
Main classification: G01N27
IPC codes: G01N27