PHOSPHORYLATION PROCESS AND CATALYST

Abstract: A process and use of at least one catalyst for phosphorylation, in particular phosphitylation, of hydroxyl groups, whereby a phosphorous compound is added to a hydroxyfunctional compound having at least one hydroxyl group. The addition yields a phosphorylated product having at least one O-P bond. The catalyst employed in the process is of the general formula (R<1>)nX(R<2>)4-n, wherein each R<1> independently is hydrogen, at least one hydrocarbyl, amino, halogenated and/or silylated hydrocarbyl group, X is Si, Ge or Sn, each R<2> independently is a leavening group and is F, Cl, Br, I or a sulphonate group optionally being halogen substituted and wherein n is 1, 2 or 3.

Description: PHOSPHORYLATION PROCESS AND CATALYST The present invention relates to a process and use of at least one Si, Ge or Sn comprising catalyst for phosphorylation, in particular phosphitylation, of hydroxy] groups A phosphorous compound is added to a hydroxyfunctional compound having at least one hydroxyl group and said addition yields in the presence of said catalyst a phosphorous reaction product having at least one O-P bond. The manufacture and use of phosphorous compounds, such as phosphates, phosphites and phosphides, are of substantial importance in areas including various animal diets, fertilisers, detergents, pharmaceuticals, plasticisers, antioxidants, flame retardants and the like. Phosphorylation of hydroxyfunctional compounds is known to be performed using phosphorous acids, whereby the phosphorus containing groups react with hydroxyl groups and other similar procedures. Phosphorylations comprising a reaction between for instance an amide of a phosphorous acid and a hydroxyfunctional compound, as illustrated by the simplified reaction scheme (a) below Reaction Scheme (a) wherein each R independently can be alkyl, cycloalkyl, aryl, alkoxy, cycloalkoxy, aryloxy. thiolo and the like, proceed very slowly and with exceptionally low yield, often as low as 20% or less, and a catalyst is normally used A frequently used catalyst is tetrazole. derivatives thereof and structural analogues The yield is thereby increased, but still very low, normally less than 50% The use of tetrazole and its derivatives as catalysts exhibit, besides the low yield a number of drawbacks, such as ♦ the amount of tetrazole must be optimised in every case, ♦ excess of tetrazole must be used, ♦ formation of side products, usually derivatised from disproportionations, ♦ problems caused by separation, and ♦ freshly sublimed tetrazole must be used, ♦ tetrazole is very expensive making large scale syntheses impossible ♦ tetrazole is difficult, inconvenient and hazardous to handle. Through the present invention, it has quite unexpectedly been found that a range of compounds, not previously employed as catalysts in phosphitylations and phosphorylations, substantially improve reaction time and yield. The yield is for instance in comparison to tetrazole increased from less than 50% to 80- 100%, normally 90-95%. The present invention refers to a process for phosphorylation, in particular a phosphitylation, of hydroxyl groups and use of at least one Si, Ge or Sn comprising catalyst during said process, whereby a phosphorous compound such as an amide of a trivalent phosphorous acid reacts with a hydroxyfunctional compound yielding a phosphorous reaction product having at least one O-P bond. The process is performed in the presence of at least one catalyst of general formula (1): (R^XrR 2 )^ Formula (1) wherein i) each R independently is a) hydrogen, b) at least one alkanyl, alkenyl, alkynyl, cycloalkanyl, cycloalkenyl, cycloalkynyl, aryl, alkanylaryl, alkenylaryl, alkynylaryl, trityl, alkoxy, cycloalkoxy, aryloxy or amino group, c) a polymeric moiety derived from a carbon polymer or copolymer, d) at least one halogenated and/or silylated alkanyl. alkenyl. alkynyl, cycloalkanyl, cycloalkenyl. cycloalkynyl. aryl, alkanylaryl, alkenylaryl, alkynylaryl, trityl, alkoxy, cycloalkoxy. aryloxy or amino group, and/or e) a polymeric moiety derived from a halogenated and/or silylated carbon polymer or copolymer; ii) X is Si, Ge or Sn; iii) each R " independently is a leavening group and is a) F, Cl, Br or 1; or b) a sulphonate group of genera! formula (2) o —0—8— R3 Formula (2) S wherein R is F, Cl, Br, I, alkanyl, alkenyl, alkynyl, aryl, arylalkanyl, arylalkenyl, arylalkynyl, haloalkanyl, haloalkenyl, haloalkynyl, haloaryl, haloarylalkanyl, haloarylalkenyl, haloarylalkynyl, arylhaloalkanyl, 4 4 arylhalbalkenyl, arylhaloalkynyl or a group of formula CR , wherein R is F 3 , Cl 3 , Br 3 or I 3 ; iv) n is 1 , 2 or 3. The reaction performed during the process and activated by the compound of Formula (I) can be exemplified by below reaction scheme (b) illustrating the phosphitylation of thymidine using trimethyichlorosilane as catalyst: Reaction Scheme (b) Activation by a catalyst in accordance with the present invention requires normally one equivalent or less. The reaction is fast, often 30 minutes to 2 hours at 20°C, and free of side products The yield is excellent, in most cases 95- 100%. and the resulting products are essentially pure requiring no further purification In preferred embodiments of the invention is each R individually methyl, ethyl, butyl, propyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, methylethyl, methylbutyl, methylisobutyl, methylpropyl, methylisopropyl, methylocty! methylphenyl, methyltrityl, allylmethyl. allylethyl, ethylbutyl, ethylisobutyl, ethylpropyl, ethylisopropyl, butylphenyl, butyl ethoxyphenyl, ethoxy, propoxy, butoxy, ethoxymethyl, ethoxyethyl, thexylmethyl, phenyl, benzyl, xylyl, thexyl, thexylethyl, methyltrityl, butylmethylene, butylphenoxymethyl, methoxymethyl, vinyl, vinylmethyl, vinylethyl, vinylethoxy, cyanomethyl, cyanoethyl, halomethyl, haloethyl, halobutyl, halopropyl, halopentyl, halohexyl, haloheptyl, haloctyl, halononyl, halodecyl, halophenyl, halobenzyl, haloxylyl, halothexyl, methyl- halohexyl, halophenylmethyl, butylhalophenylmethyl, halovinyl, vinylhalomethyl or vinylhaloethyl. At least one R can in these and other preferred embodiments can be a moiety derived from a carbon polymer or copolymer, such as polyethylene, a polystyrene, a polyether or a polyester, which polymer optionally is silylated and/or halogenated. X is in these and other especially preferred embodiments preferably Si. Employed catalyst is in especially preferred embodiments a trialkylhalosilane, such as a trialkylchlorosilane, trialkyliodosilane, trialkylbromosilane or trialkyl- fluorosilane preferably selected from the group consisting of trimethylchlorosilane, triethylchlorosilane, tributylchlorosilane, tripropylchlorosilane, trimethyliodosilane, triethyliodosilane, tributyliodosilane, tripropyliodosilane, trimethylbromosilane, ' triethylbromosilane, tributylbromosilane, tripropylbromosilane, trimethylfluoro- silane, triethylfluorosilane, tributylfluorosilane, tripropylfluorosilane. Further compounds, advantageously being used as catalysts in accordance with the present invention, of Formula (I) wherein X is Si are suitably exemplified by trimethylchlorosilane, trimethylbromosilane, trimethyliodosilane, trimethyl- silyltrichloroacetate. trimethylsilyltrifluoroacetate, allyldimethylchlorosilane, bromomethyldimethylchlorosilane, tert-butyldimethylchlorosilane, -tert - butyldimethylsilyl- -methyltrifluoroacetamide. N.O-bis( trimethylsilyl Urifluoro- acetamide. N -methy 1-N-trimethylsilyl-heptafluorobutyramide. N-methyl-N- trimethylsilyltrifluoroacetamide, trimethyl silyltrifluoromethanesulphonate. tert- butyldirnethylsilyltrifluoromethanesulphonate, tert-butyldiphenylchlorosilane. tert - butyl-methoxy-phenylbromosilane, chloromethyldimethylchlorosilane dimethyl- (3 ,3,4,4,5, 5,6,6,6-nonaf uorohexyl)chlorosilane, dimethylphenylchlorosilane, dimethyltπtylbromosilane, diphenylmethylchlorosilane, lsopropyldimethyl- chlorosilane, (pentafluorophenyl)dιmethylchlorosilane, thexyldimethylchlorosilane, thexyldimethylsilyl trifluoromethanesulphonate, tπbutylchlorosilane, tπethyl- chlorosilane, tπthylsilyltπfluoromethanesulphonate, triisopropylchlorosilane, trnsopropylsilyltrifluoromethanesulphonate, tπphenylchlorosilane, tπpropylchloro- silane, di-tert-butyldichlorosilane, di-tert-butylsilyl bιs(trifluoromethane- sulphonate), diethyldichlorosilane, diιsopropylsilyl-bιs(tπfluoromethane- sulphonate), dimethyldichlorosilane, diphenyldichlorosilane, methylphenyl- dichlorosilane, dimethyloctylchlorosilane, dodecyltrichlorosilane thexyl- dimethylsilyl-trifluoromethanesulphonate, thexyl-dimethylchlorosilane, trichloro- silan, 3-(triethylsilyl)propyl-tπmethylammoniumchloride, tπmethylsilylbromo- acetate, tπmethylsilylchlorosulphonate, 2-(tπmethylsilyl)ethoxymethyl chloride, tri(dimethylamino)sulphonium-difluorotπmethylsilicate and l,3-bιs(chloromethyl)- 1 , 1,3,3 -tetramethyldisilazane One or more solvents are suitably present during the process of the present invention The solvent is preferably selected from the group consisting of aliphatic solvents, cydoaliphatic solvents, aromatic solvents, 1 ,3-dioxanes, 1 ,3-dixolanes, 1,3,5-tπoxepanes, furans and/or haloalkanes, such as hexane, toluene, xylene, tetrahydrofuran, dichloromethane and acetonitrile The process of the invention and the catalyst used therein are suitable for phosphorylation, in particular phosphitylation, of hydroxyfunctional compounds such as mono, di, tn and polyfunctional alcohols, monosaccharides, disaccharides, polysaccharides, sugar alcohols, cyclodextπns, inositols, nucleosides, terpenoids, lipids, phenols, polyphenols, hydroxyfunctional polymers hydroxyfunctional carboxylic acids and/or deπvatives of said hydroxyfunctional compounds The hydroxyfunctional compound is advantageously selected from the group consisting of a) pentoses hexoses and heptoses such as D/L-πbose D/L-arabinose D/L-xylose, D/L-lyxose, D/L-allose, D/L-altrose. D/L-glucose, D 'L-mannose D/L-gulose, D-L-idose, D/L-galactose D/L-talose D/L-glucoheptose D/L-mannoheptose, D/L-ribulose, D/L-xylulose, D/L-psicose, D/L-fructose, D/L-sorbose, D/L-tagatose, D/L-sedoheptulose and/or derivatives of said pentoses, hexoses and heptoses, b) pentitols, hexitols and heptitols such as D/L-ribitol, D/L-arabinitol, D/L-xylitol, D/L-lyxitol, D/L-allitol, D/L-altritol, D/L-glucitol, D/L-mannitol, D/L-gulitol, D/L-iditol, D/L-galactitol, D/L-talitol, D/L-glucoheptitol, D/L-mannoheptitol, D/L-ribulitol, D/L-xylulitol, D/L-psicitol, D/L-fructitol, D/L-tagatitol, D/L-sedoheptulitol and derivatives of said pentitols, hexitols and heptitols, c) anhydropentitols, anhydrohexitols, anhydroheptitols and derivatives thereof, d) cellobiose, maltose, lactose, saccharose, gentobiose, melibiose, trehalose, turanose and derivatives thereof, e) starch, glycogen, cellulose, dextran, tunicine and/or derivatives thereof, and f) myo-inositol, cis-inositol, epi-inositol, allo-inositol, neo-inositol, muco-inositol, D/L-chiro-inositol, scyllo-inositol and derivatives of said inositols, g) 5-ethyl-5-methanol-l ,3-dioxane, 2-ethyl-2-butyl-l ,3-propanediol, neopentyl glycol, glycerol, trimethylolpropane, trimethylolethane, pentaerythritol, diglycerol, ditrimethylolpropane, ditrimethylolethane, dipentaerythritol, tripentaerythritol and derivatives thereof, h) 2,2-dimethylolpropionic acid, α,α-bis-(hydroxymethyl)butyric acid, α,α,α-tris(hydroxymethyl)acetic acid, α,α-bis-(hydroxymethyl)valeric acid, α,α-bis(hydroxy)propionic acid, 3,5-dihydroxybenzoic acid, α,β-dihydroxy- propionic acid, heptonic acid, citric acid, tartaric acid, dihydroxymaloic acid, gluconic acid and derivatives thereof. The hydroxyfunctional compound can also suitably be a dendritic or hyperbranched macromolecule. Hyperbranched and dendritic macromolecules normally consist of an initiator or nucleus having one or more reactive sites and a number of surrounding branching layers optionally being chain terminated. The layers are usually called generations, whereby each generation comprises one or more branches, usually called dendrons. A macromolecule suitable for a phosphitylation according to the present invention can be composed of a monomeric or polymeric nucleus to which 1 - 100, preferably 1 -20, generations consisting of at least one monomeric or polymeric chain extender are added The chain extender has at least one, preferably at least two, reactive hydroxyl groups and at least one reactive carboxyl group The terminal functions of yielded macromolecule is, prior to the optional chain termination, substantially hydroxyl groups An optional chain termination is performed in such a manner and to such a degree that the macromolecule finally has at least one terminal hydroxyl group The nucleus may be a compound, such as an alcohol, a polyalcohol, a glycidyl ester, a glycidyl ether or the like having at least one reactive hydroxyl or epoxide group In further embodiments, the nucleus may be a metal ion or an organometallic compound being hydroxy and/or epoxide functional or the like. Hyperbranched and dendritic macromolecules (dendrimers) can generally be described as three dimensional highly branched molecules having a tree-like structure Dendrimers are highly symmetric, while similar macromolecules designated as hyperbranched may to a certain degree hold an asymmetry, yet maintaining the highly branched tree-like structure. Dendrimers can be said to be monodisperse variations of hyperbranched macromolecules. The composition of hyperbranched dendritic or near dendritic macromolecule can be illustrated by below simplified Formula (3. 1 ) and (3.2) illustration a hyperbranched macromolecule (Formula 3. 1 ) and a dendron (Formula 3.2) being part of the macromolecule. Regard is not taken to any three dimensional structure. The macromolecule has two generations of chain extenders (A and B) having 3 and 4 reactive sites The reactive sites are one carboxyl group, reacted with the nucleus (Y), and hydroxyl groups. The macromolecule is partly chain terminated by means of a chain terminator (T). The nucleus or initiator (Y) is as previously defined Formula 3 1 Formula 3 2 The hydroxyfunctional compound can, furthermore, be a dendron of for instance general formula (4) R 1 2 (OOR π ) q (OOR 1 ) N (OOR 15 ) wherein each R 1 : , R p . R 14 and R independently is alkanyl, alkenyl, alkynyl, cycloalkanyl, cycloalkenyl, cycloalkynyl, aryl, alkanylaryl, alkenylaryl or alkynylaryl, q is an integer and at least 1 , y is 0 or an integer and at least 1 and wherein z is an integer between 1 and 25, preferably between 1 and 10. A suitable dendron can be exemplified by self condensated 2,2-dimethylolpropionic acid, whereby a suitable number of moles of said compound is condensated (esterified) at a temperature of for instance 150-250°C 2,2-Dimethylolpropionic acid has two hydroxyl groups and one carboxyl group and one molecule of the compound can thus react with two other molecules, whereby a highly branched chain consisting of esterified 2,2-dimethylolpropionic can be prepared The phosphorous compound used in the phosphorylation process is advantageously an amide of a phosphorous acid containing trivalent phosphorus, which compound is of general formula (5), (6) or (7), RVPNRV, R 6 P(NR 7 R 8 ) 2 or P(NR 7 R 8 ) 3 , wherein each R and R independently is an alkyl, a cycloalkyl, an aryl, an alkoxy, 7 8 a cycloalkoxy, an aryloxy, a thiolo or an amido group and wherein each R and R independently is an alkyl, a cycloalkyl or an aryl group. The phosphorous compound can alternatively be of general formula (8) or (9), (R CH 2 O) 2 PNR R or (NCCH 2 CH 2 O) 2 PNR 10 R n , wherein each R 9 independently is aryl or alkaryl and wherein each R and R independently are C j -C 12 alkyl Said phosphorous compounds can be exemplified by neopentylene-N,N-dimethylphosphoroamidite, o-phenylene-N,N-diisopropylphosphoroamidite, di-(2-cyanoethyl)-N,N-diisopropyl- phosphoroamidite, 2-cyanoethyl-N,N-diisopropylfluorophosporoamidite, dibenzyl- N,N-diethylphosphoroamidite and hexamethylphosphorotriamide A phosphitylation process according to the present invention is preferably performed at a temperature of -80°C to 120°C, such as - 10°C to 80°C or 0°C to 40°C, using a catalyst amount corresponding to 0 01 to 3 , preferably 0 05 to 1 and most preferably 0 1 to 0 5, equivalents calculated on phosphorous equivalents The amount of catalyst is normally between 5% and 80% A suitable and preferred order of addition is £ the phosphorous compound or compounds. 2 the hydroxyfunctional compound or compounds and 3_ the catalyst or catalysts The phosphorylated, in particular phosphitylated, reaction product obtained in the process and by using the catalyst of the present invention can optionally be further processed Trivalent phosphorus included in the reaction product can for example be oxidised to pentavalent phosphorus using an oxidising agent, such as a peroxide, a hydroperoxide, a peracid, or by means of sulphur transferring reagents including elemental sulphur Phosphites can thus be transformed into phosphates yielding for instance pharmaceutically active and important trisphosphates of monosaccharides and inositols. Furthermore, β-cyanoalkyl groups included in the phosphorylated product can be eliminated in the presence of a base, such as potassium hydroxide, sodium hydroxide or an amine and benzyl groups by means of a hydrogefrolysis The present invention makes it possible to employ simple phosphitylation reagents of for instance formula (8) or (9) for phosphitylation of 3 '-protected nucleosides, 5'-protected nucleosides and other alcoholic compounds of biological importance, such as sugars, terpenoids and lipids Oxidisation of phosphites to phosphates, optional removal of β-cyanoalkyl and benzyl groups, result in a phosphate yield of normally more than 90%. Further application areas of the process of present invention and catalyst used therein are ♦ preparation of oligomeric and polymeric dendrons from polyols by a suitable choice of phosphitylation reagent, ♦ preparation of antioxidants containing a nucleus of trivalent phosphorus, ♦ preparation of intermediates containing trivalent phosphorus, which can be converted into tetravalent or pentavalent structures resulting in important biophosphates and their structural analogues as well as material improving components, such as plasticisers and flame retardants, ♦ preparation of olefinic monomers containing trivalent and pentavalent phosphorus, which monomers when heated in the presence of for instance benzoyl peroxide give flame retardant solids, and ♦ preparation of detergents These and other objects and the attendant advantages will be more fully understood from the following detailed description, taken in conjunction with embodiment Examples 1 - 1 1 , whereby said Examples present Example 1 Phosphitylation of uridine - Comparison between (t) no catalyst, (it) tetrazole and (in) catalyst according to the present invention Example 2 Synthesis of ethyl neopentylene phosphite Example 3 Synthesis of ethyl o-phenylene phosphite Example 4 Synthesis of thymidine cyclic 3',5'-N,N-dimethylphosphoroamidite Example 5 Synthesis of (±) 2,3,6-tri-O-benzyl-myo-inositol- l ,4,5-tris[di-(2- cyanoethyl) phosphite]. Example 6 Synthesis of α-methyl-6-O-acetyl-D-mannopyranoside-2,3,4-tris- (dibenzyl phosphite). Example 7 Synthesis of (±) 3,4,5,6-tetra-O-benzyl-myo-inositol- l,2-bis(di- benzyl phosphite). Example 8 Synthesis of (±) 3,4,5-tri-O-benzyl-myo-inositol-l,2,6-O-tris(di- benzyl phosphite). Example 9 Synthesis of (±) 3,4,5,6-tetra-O-benzyl-myo-inositol-l,2-bis(2- cyanoethyl fluorophosphite) Example 10 Oxidisation the phosphite of Example 6 yielding corresponding phosphate Example 11 Elimination of benzyl groups in included in the phosphate of Example 10 EXAMPLE 1 Phosphitylation of thymidine according to the following reaction scheme was repeatedly performed using i) no catalyst, dioxane as solvent and 80 C C as reaction temperature, ti) a 6 fold excess of tetrazole (calculated on P) as catalyst, dioxane as solvent and 80°C as reaction temperature, ui) 0.25 equivalent of trimethylchlorosilane (calculated on P) as catalyst, dioxane as solvent and 20°C as reaction temperature, giving the following average yield of cyclic amidite i) =20% after 8 hours of reaction, it) 25-30%) after 6 hours of reaction in) >95% after 2 hours of reaction, whereby i) and it) are comparative phosphitylations outside the scope of the invention and iii) is a phosphitylation in accordance with the invention. Above result clearly shows the great advantages obtained when using a phosphitylation process according to the present invention, such as a substantially - increased yield, - reduced amount of catalyst, - reduced reaction temperature and time, - reduced cost, and - improved environmental and handling safety. EXAMPLE 2 34.5 g (0.2 mole) of neopentylene-N,N-dimethylphosphoroamidite, 9 7 g (0.21 mole) of ethanol and 150 ml sodium dried tetrahydrofuran were charged in a 4-necked reaction flask equipped with a stirrer, a thermometer, an instillation funnel and an inlet for inert gas The mixture was stirred and allowed to form a solution to which 5.4 g (0.05 mole) trimethylchlorosilane was instilled at such a rate that the temperature never exceeded 20°C Stirring was maintained during the instillation and cooling of the reaction flask was applied if necessary The reaction mixture was stirred and kept at 20°C for a further 30 minutes Solvent, excess of ethanol and unchanged trimethylchlorosilane were now removed in vacuo The residue was vacuum distilled to give 30 4 g of ethyl neopentylene phosphite Yield 85%, Bp 16 77-78 c C. n D :o 1 4437 All operations were, in order to secure a high yield and product purity, performed under a blanket of nitrogen free of oxygen and moisture EXAMPLE 3 47 8 g (0 2 mole) of o-phenylene-N,N-dιisopropylphosphoroamidιte, 9 7 g (0 21 mole) of ethanol and 150 ml sodium dried tetrahydrofuran were charged in a 4-necked reaction flask equipped with a stirrer, a thermometer, an instillation funnel and an inlet for inert gas The mixture was stirred and allowed to form a solution to which 5 4 g (0 05 mole) trimethylchlorosilane was instilled at such a rate that the temperature never exceeded 20°C Stirring was maintained during the instillation and cooling of the reaction flask was applied if necessary The reaction mixture was stirred and kept at 20°C for a further 30 minutes Solvent, excess of ethanol and unchanged trimethylchlorosilane were now removed in vacuo and 35.0 g of ethyl ø-phenylene phosphite Analysis indicated that obtained product had a purity of at least 96% Yield 95%, 31 P NMR δ: -129 ppm (neat), n D 20 - 1 4437 All operations were, in order to secure a high yield and product purity, performed under a blanket of nitrogen free of oxygen and moisture EXAMPLE 4 20 ml of sodium dried tetrahydrofuran was charged in a 50 ml flask (dried at 300°C) equipped with a magnetic stirrer and filled with dry oxygen free argon The flask was covered with a rubber septum and 0 134 (0 83 mmole) of- hexamethylphosphorotriamide and 0 076 g (0 83 mmole) of trimethylchlorosilane were added at 20°C and stirred 0 2 g (0.83 mmole) of thymidine was after 5 minutes of stirring added at 20°C and the reaction mixture was stirred at 20°C for a further 60 minutes Solvent and catalyst were now evaporated in vacuo and 0 3 g (yield 98%) of crude thymidine cyclic 3',5'-dimethylphosphoroamidιte was obtained The crude product was dissolved in 5 ml of CH 2 Gι and purified by column chromatography on silica gel using CH ; CL/CH-,COCH 10 3 as eluent The purification resulted in 0 28 g of pure thymidine cyclic 3'.5'-dιmethyl- phosphoroamidite Yield 95%. 31 P NMR (C 6 D 6 ) δ 1 38 27, 1 37 91 EXAMPLE 5 20 ml of sodium dried tetrahydrofuran was charged in a 50 ml flask (dried at 300°C) equipped with a magnetic stirrer and filled with dry oxygen free argon The flask was covered with a rubber septum and 3 mmole of di-(2-cyanoethyl)-N,N-diisopropylphosphoroamidite and 3 mmole of trimethyl¬ chlorosilane were added at 20°C and stirred A solution of 1 mmole of (±) 1 ,2,4-tri-O-benzyl-myo-inositol in 10 ml of sodium dried tetrahydrofuran was after 5 minutes of stirring added at 20°C and the reaction mixture was stirred at 20°C for a further 2 hours Solvent and catalyst were now evaporated in vacuo, yielding crude (±) 2,3,6-tri-O-benzyl-myo-inositol- l ,4,5-tris[di-(2-cyanoethyl)phosphite) Yield 95%7 31 P NMR (CDC1 3 ) δ: 139.6; 137 91 , P- l , 140.2, 141 .0, P-4, ( 5 J pp =3 4 Hz). EXAMPLE 6 20 ml of sodium dried tetrahydrofuran was charged in a 50 ml flask (dried at 300°C) equipped with a magnetic stirrer and filled with dry oxygen free argon The flask was covered with a rubber septum and 3 mmole of dibenzyl-N,N-diethyl- phosphoroamidite and 0.05 mmole of trimethylchlorosilane were added at 20°C and stirred A solution of 1 mmole of α-methyl-6-O-acetyl-D-mannopyranoside in 10 ml of sodium dried tetrahydrofuran was after 5 minutes of stirring added at 20°C and the reaction mixture was stirred at 20°C for a further 2 hours Solvent and catalyst were now evaporated in vacuo The residue was purified by column chromatography on silica gel 60 (230-400 mesh) using hexane/dietylether as eluent The purification resulted in 0 87 g of pure α-methyl-6-O-acetyl-D- mannopyranoside-2,3,4-tris(dibenzylphosphite) Yield 89%, 31 P NMR (C 6 D 6 ) δ 140 3 , 141 6 (2P) EXAMPLE 7 20 ml of sodium dried tetrahydrofuran was charged in a 50 ml flask (dried at 300°C) equipped with a magnetic stirrer and filled with dry oxygen free argon The flask was covered with a rubber septum and 2 mmole of dibenzyl-N. -diethyl- phosphoroamidite and 2 mmole of trimethylchlorosilane were added at 20°C and stirred A solution of 1 mmole of (±) 3.4,5,6-tetra-O-benzyl-myo-inosιtol in 1 0 ml P / E97/00345 of sodium dried tetrahydrofuran was after 5 minutes of stirring added at 20°C and the reaction mixture was stirred at 20°C for a further 2 hours Solvent and catalyst were now evaporated in vacuo The residue was purified by column chromatography on silica gel 60 (230-400 mesh) using hexane/diethylether 8 1 as eluent The purification resulted in 0 82 g of pure (±) 3,4,5,6-tetra-O- benzyl-myo-inositol- l ,2-bis(dibenzylphosρhite) Yield 80%, 31 P NMR (C 6 D 6 ) δ 140 5 (d, J= 1 .8 Hz), 140 6 (d, J=1 .8 Hz) EXAMPLE 8 20 ml of sodium dried tetrahydrofuran was charged in a 50 ml flask (dried at 300°C) equipped with a magnetic stirrer and filled with dry oxygen free argon The flask was covered with a rubber septum and 3 mmole of dibenzyl-N,N-diethyl- phosphoroamidite and 3 mmole of trimethylchlorosilane were added at 20°C and stirred. A solution of 1 mmole of (±) 3,4,5-tri-O-benzyl-myo-inositol in 10 ml of sodium dried tetrahydrofuran was after 5 minutes of stirring added at 20°C and the reaction mixture was stirred at 20°C for a further 2 hours. Solvent and catalyst were now evaporated in vacuo. The residue was purified by column chromatography on silica gel 60 (230-400 mesh) using hexane/diethylether 5 : 1 as eluent. The purification resulted in 1.2 g of pure (±) 3,4,5-tri-O- benzyl-myo-inositol-l,2,6-tris(dibenzylphosphite): Yield 100%; 31 P NMR (C 6 D 6 ) δ: 140.2 (d, J P1P2 2.1 Hz), 140 8 (dd, J P1P2 2.1 Hz, J P1 P6 3.9 Hz, P- l), 143.2 (d, J pι P6 . 3.9 Hz, P-6) EXAMPLE 9 20 ml of sodium dried tetrahydrofuran was charged in a 50 ml flask (dried at 300°C) equipped with a magnetic stirrer and filled with dry oxygen free argon. The flask was covered with a rubber septum and 0 86 mmole of 2-cyanoethyl-N.N-diisopropylfluorophosphoroamidite and 0 86 mmole of trimethylchlorosilane were added at 20°C and stirred A solution of 0 4 mmole of (±) 3,4,5.6-tetra-O-benzyl-myo-inositol in 10 ml of sodium dried tetrahydrofuran was after 5 minutes of stirring added at 20°C and the reaction mixture was stirred at 20°C for a further 2 hours Solvent and catalyst were now evaporated in vacuo The residue was purified by column chromatography on silica ge! 60 (230-400 mesh) using CH„CL/CH 3 COCH 3 10:3 as eluent. The purification resulted in 0.23 g of pure (±) 3,4,5,6-tetra-0-benzyl-myo-inositol- l ,2-bis(2-cyanoethylphosphite) Yield 80%, 31 P NMR (CDCl λ ) δ : 138.6 m, 123 6 m EXAMPLE 10 0.50 g of -methyl-6-0-acetyl-D-mannopyranoside-2,3,4-tris(dibenzylphosphite), obtained in Example 6, was dissolved in 20 ml of dichloromethane. 0.97 g of w-chloroperbenzoic acid in 20 ml of dichloromethane was now instilled during 1 hour. The temperature was adjusted to 20°C and maintained for a further 1.5 hour followed by an evaporation of the solvent. The residue was by NMR identified to be α-methyl-6-O-acetyl-D-mannopyranoside-2,3,4-tris(dibenzylphosphate). EXAMPLE 11 0.35 g of α-methyl-6-O-acetyl-D-mannopyranoside-2,3,4-tris(dibenzylphosphate), obtained in Example 10, was dissolved in 5 ml of ethanol and hydrogenated for 10 minutes at 1 bar and 20°C over 0.10 g of 5% Pd/C. The mixture was evaporated and the residue was by NMR identified to be α-methyl-6-O-acetyl-D- mannopyranoside-2,3,4-trisphosphate. While particular embodiments of the invention have been shown, it will be understood, of course, that the invention is not limited thereto since many modifications may be made, and it is, therefore, contemplated to cover by the appended claims any such modifications as fall within the true spirit and scope of the invention.

Claims: CLAIMS A process for phosphorylation, in particular phosphitylation, of hydroxyl groups, whereby a phosphorous compound is added to a hydroxyfunctional compound having at least one hydroxyl group, which addition yields a phosphorous reaction product having at least one O-P bond c h a r a c t e r i s e d i n, that said phosphorylation is performed in the presence of at least one catalyst of general formula (R ) n X(R ) 4 . n , wherein i) each R independently is a) hydrogen, b) at least one alkanyl, alkenyl, alkynyl, cycloalkanyl, cycloalkenyl, cycloalkynyl, aryl, alkanylaryl, alkenylaryl, alkynylaryl, trityl, alkoxy, cycloalkoxy, aryloxy or amino group, c) a polymeric moiety derived from a carbon polymer or copolymer, d) at least one halogenated and/or silylated alkanyl, alkenyl, alkynyl, cycloalkanyl, cycloalkenyl, cycloalkynyl, aryl, alkanylaryl, alkenylaryl, alkynylaryl, trityl, alkoxy, cycloalkoxy, aryloxy or amino group, or e) a polymeric moiety derived from a halogenated and/or silylated carbon polymer or copolymer; ii) X is Si, Ge or Sn; iii) each R independently is a leavening group and is a) F, Cl, Br or I; or b) a sulphonate group of formula O — O— S— R3 o wherein R " is F, Cl, Br, I, alkanyl, alkenyl, alkynyl, aryl, arylalkanyl, arylalkenyl. arylalkynyl, haloalkanyl, haloalkenyl. haloalkynyl, haloaryl, haloarylalkanyl, haloary alkenyl. haloarylalkynyl, arylhaloalkanyl, arylhaloalkenyl, arylhaloalkynyl or A 4 a group of formula CR . wherein R is F CL, Br ? or 1 , . and iv) n is 1 , 2 or 3. 2. A process according to claim 1 ch a racte rised in, that each R individually is methyl, ethyl, butyl, propyl, pentyl, hexyl, heptyl, octyl. nonyl, decyl, methylethyl, methylbutyl, methylisobutyl, methylpropyl, methylisopropyl, methyloctyl methylphenyl, methyltrityl, allylmethyl, allylethyl, ethylbutyl, ethylisobutyl, ethylpropyl, ethylisopropyl, butylphenyl, butylmethoxyphenyl, ethoxy, propoxy, butoxy, ethoxymethyl, ethoxyethyl, thexylmethyl, phenyl, benzyl, xylyl, thexyl, thexylethyl, methyltrityl, butylmethylene, butylphenoxymethyl, methoxymethyl, vinyl, vinylmethyl, vinylethyl, vinylethoxy, cyanomethyl, cyanoethyl, halomethyl, haloethyl, halobutyl, halopropyl, halopentyl, halohexyl, haloheptyl, haloctyl, halononyl, halodecyl, halophenyl, halobenzyl, haloxylyl, halothexyl, methylhalohexyl, halophenylmethyl, butylhalophenylmethyl, halovinyl, vinylhalomethyl or vinylhaloethyl. 3. A process according to claim 1 or 2 chara cterised in, that at least one R is a moiety derived from a polymer or copolymer, such as polyethylene, a polystyrene, a polyether or a polyester. 4. A process according to claim 3 ch ara cterised in, that said polymer is silylated and/or halogenated. 5. A process according to any of the claims 1-4 c haracterised in, that Xis Si. 6. A process according to any of the claims 1-5 c h ara c terised in, that the catalyst is a trialkylhalosilane. 7. A process according to claim 6 c h a ra c teris ed i n, that the trialkylhalosilane is a trialkylchlorosilane, trialkyliodosilane, trialkyl- bromosilane or trialkylfluorosilane 8. A process according to claim 6 or 7 c hara cterised in, that the catalyst is trimethylchlorosilane, triethylchlorosilane, tributylchlorosilane, tripropylchlorosilane, trimethyliodosilane, triethyliodosilane, tributyl- iodosilane, tripropyliodosilane, trimethylbromosilane, triethylbromosilane, tributylbromosilane, tripropylbromosilane, trimethylfluorosilane, triethyl- fluorosilane, tributylfluorosilane, tripropylfuorosilane. 9. A process according to any of the claims 1-8 chara cterised in, that at least one solvent is present during said phosphitylation. 10. A process according to claim 9 characterised in, that the solvent is an aliphatic solvent, a cydoaliphatic solvent, an aromatic solvent, a 1,3-dioxane, a 1,3-dixolane, a 1,3,5-trioxepane, a furan and/or a haloalkane. 11. A process according to claims 10 characterised in, that the solvent is hexane, toluene, xylene, tetrahydrofuran, dichloromethane or acetonitrile. 12. A process according to any of the claims 1-11 characteris ed in, that the hydroxyfunctional compound is an alcohol, a polyalcohol, a monosaccharide, a disaccharide, a polysaccharide, a sugar alcohol, a cyclodextrin, an inositol, a nucleoside, a terpenoid, a lipid, a phenol, a polyphenol, a hydroxyfunctional dendritic or hyperbranched macromolecule, a hydroxyfunctional polymer, a hydroxyfunctional carboxylic acid and/or a derivative of said hydroxyfunctional compounds 13. A process according to claim 12 cha ra cte ris ed i n. that the hydroxyfunctional compound is a pentose, a hexose or a heptose selected from the group consisting of D/L-ribose. D/L-arabinose. D/L-xylose, D/L-lyxose, D/L-allose, D/L-altrose, D/L-glucose, D/L-mannose, D/L-gulose, D/L-idose, D/L-galactose, D/L-talose, D/L-glucoheptose, D/L-mannoheptose, D/L-ribulose, D/L-xylulose, D/L-psicose, D/L-fructose, D/L-sorbose, D/L-tagatose, D/L-sedoheptulose and/or a derivative of said pentoses, hexoses or heptoses. 14. A process according to claim 12 ch a racterised in, that the hydroxyfunctional compound is a pentitol, a hexitol or a heptitol selected from the group consisting of D/L-ribitol, D/L-arabinitol, D/L-xylitol, D/L-lyxitol, D/L-allitol, D/L-altritol, D/L-glucitol, D/L-mannitol, D/L-gulitol, D/L-iditol, D/L-galactitol, D/L-talitol, D/L-glucoheptitol, D/L-mannoheptitol, D/L-ribulitol, D/L-xyhilitol, D/L-psicitol, D/L-fructitol, D/L-tagatitol, D/L-sedoheptulitol and/or a derivative of said pentitols, hexitols or heptitols. 15. A process according to claims 14 characterised in, that said pentitols, hexitols and heptitols are anhydropentitols, anhydrohexitols, anhydroheptitols and/or derivatives thereof. 16. A process according to claim 12 characterised in, that the hydroxyfunctional compound is an inositol selected from the group ' consisting of myo-inositol, cis-inositol, epi-inositol, allo-inositol, neo-inositol, muco-inositol, D/L-chiro-inositol, scyllo-inositol and/or a derivative of any of said inositols. 17. A process according to claim 12 cha ra cterised i n, that the hydroxyfunctional compound is a mono, di, tri or polyfunctional alcohol, preferably selected from the group consisting of 5-ethyl-5-methanol- 1 ,3 -dioxane, 2-ethyl-2-butyl- 1 ,3-propanediol, neopentyl glycol, glycerol, trimethylolpropane, trimethylolethane. pentaerythritol, diglycerol, ditrimethylolpropane. ditrimethylolethane, dipentaerythritol or tripentaerythritol /00345 18. A process according to claim 12 c ha ra cte ris e d in, that the hydroxyfunctional compound a hydroxyfunctional acid, such as 2,2-dimethylolpropionic acid, 3,5-dihydroxybenzoic acid, heptonic acid or citric acid. 19. A process according to claim 12 cha racterised i n, that the hydroxyfunctional compound is a dendritic or hyperbranched macromolecule composed of a monomeric or polymeric nucleus having at least one -reactive epoxide or hydroxyl group to which 1-100, preferably 1-20, generations of at least one monomeric or polymeric chain extender having at least one, preferably at least two, reactive hydroxyl groups and at least one reactive carboxyl group and that terminal functions of said macromolecule substantially are hydroxyl groups. 20. A process according to claim 19 characterised in, that the hyperbranched or dendritic macromolecule is partly chain terminated and that said macromolecule has at least one terminal function being a hydroxyl group 21. A process according to claim 12 cha racterised i n, that the hydroxyfunctional compound is a dendron of general formula R 12 (OOR 1 q OOR 14 ) v y(OOR 15 ).., wherein each R 12 , R 13 , R 14 and R 15 independently is alkanyl, alkenyl, alkynyl, cycloalkanyl, cycloalkenyl, cycloalkynyl, aryl, alkanylaryl, alkenylaryl or alkynylaryl, q is an integer and at least 1, y is 0 or an integer and at least 1 and wherein z is an integer between 1 and 25, preferably between 1 and 10 22. A process according to any of the claims 1-21 ch a racte rised i n, that the phosphorous compound is an amide of a phosphorous acid containing trivalent phosphorus, which compound is of general formula R R PNR R , R 6 P(NR 7 R 8 ), or P(NR 7 R 8 ) wherein each R ""1 and R 6 independently is an alkyl. a cycloalkyl, an aryl, an alkoxy, a cycloalkoxy, an aryloxy, a thiolo or 7 8 an amido group and wherein each R and R independently is an alkyl, a cycloalkyl or an aryl group. 23. A process according to any of the claims 1-21 ch aracterised in, that the phosphorous compound is of general formula (R CH.,O) 2 PNR R or (NCCH 2 CH 2 O) 2 PNR R , wherein each R independently is aryl or alkaryl and wherein each R and R independently are C,-C ]2 alkyl. 24. A process according to claim 22 or 23 c h a r a cterised in, that the phosphorous compound is selected from the group consisting of neopentylene-N,N-dimethylphosphoroamidite, o-phenylene-N,N-diisopropyl- phosphoroamidite, di-(2-cyanoethyl)-N,N-diisopropylphosphoroamidite, 2-cyanoethyl-N,N-diisopropylfluorophosporoamidite, dibenzyl-N,N-diethyl- phosphoroamidite and hexamethylphosphorotriamide. 25. A process according to any of the claims 1-24 characterised in, that said process is performed at a temperature of -80°C to 120°C, preferably -10°C to 80°C and most preferably 0°C to 40°C. 26. A process according to any of the claims 1-25 cha ra cte rised in, that the catalyst is present in an amount corresponding to 0.01 to 3, preferably 0.05 to 1 and most preferably 0.1 to 0.5, equivalents calculated on phosphorous equivalents. 27. A process according to any of the claims 1-26 c • i ra ct eris ed i n, that elimination of any β-cyanoalkyl group or groups included in yielded phosphorous reaction product is performed in presence of a base, such as potassium hydroxide, sodium hydroxide or an amine 28. A process according to any of the claims 1-27 c ha ra cteris ed in, that elimination of any benzyl group or groups included in yielded phosphorous reaction product is performed by means of a hydrogenolysis 29. A process according to any of the claims 1-28 ch a ra cte ri s ed in, that said process is a phosphitylation yielding a phosphite 30. A process according to claim 29 characterised i n, that the phosphite by means of oxidisation is transformed into a phosphate. 31. A process according to claim 30 chara cterised in, that the oxidisation is performed using at least one oxidising agents, such as a peroxide, a hydroperoxide, a peracid, or by means of a sulphur transferring reagent, such as elemental sulphur. 32. A process according to any of the claims 30 or 31 characterised in, that the phosphite is a trisphosphite of a monosaccharide, a sugar alcohol or an inositol yielding corresponding trisphosphate. 1 2 33. Use of at least one compound of general formula (R ) n X(R ) 4 _ n » wherein i) each R independently is a) hydrogen, b) at least one alkanyl, alkenyl, alkynyl, cycloalkanyl, cycloalkenyl, cycloalkynyl, aryl, alkanylaryl, alkenylaryl, alkynylaryl, trityl, alkoxy, cycloalkoxy, aryloxy or amino group, c) a polymeric moiety derived from a carbon polymer or copolymer, d) at least one halogenated and/or silylated alkanyl, alkenyl. alkynyl, cycloalkanyl, cycloalkenyl. cycloalkynyl, aryl, alkanylaryl. alkenylaryl. alkynylaryl, trityl. alkoxy, cycloalkoxy. aryloxy or amino group, or e) a polymeric moiety derived from a halogenated and/or silylated carbon polymer or copolymer, ii) X is Si, Ge or Sn; iii) each R independently is a leavening group and is a) F, Cl, Br or I; or b) a sulphonate group of formula O — O— S— R 0 wherein R is F, Cl, Br, I, alkanyl, alkenyl, alkynyl, aryl, arylalkanyl, arylalkenyl, arylalkynyl, haloalkanyl, haloalkenyl, haloalkynyl, haloaryl, haloarylalkanyl, haloarylalkenyl, haloarylalkynyl, arylhaloalkanyl, arylhaloalkenyl, arylhaloalkynyl or 4 4 a group of formula CR , wherein R is F 3 , Cl 3 , Br 3 or I 3 ; and iv) n is 1 , 2 or 3. as catalyst for phosphorylation, in particular phosphitylation, of a hydroxyfunctional compound having at least one hydroxyl group, whereby a phosphorous reaction product having at least one O-P bond is yielded 34. Use according to claim 33 c h a r a c t e r i s e d i n, that each R individually is methyl, ethyl, butyl, propyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, methylethyl, methylbutyl, methylisobutyl, methylpropyl, methylisopropyl, methyloctyl methylphenyl, methyltrityl, allylmethyl, allylethyl, ethylbutyl, ethylisobutyl, ethylpropyl, ethylisopropyl, butylphenyl, butylmethoxyphenyl, ethoxy, propoxy, butoxy, ethoxymethyl, ethoxyethyl, thexylmethyl, phenyl, benzyl, xylyl, thexyl, thexylethyl, methyltrityl, butylmethylene, butylphenoxymethyl, methoxymethyl, vinyl, vinylmethyl, vinylethyl, vinylethoxy, cyanomethyl, cyanoethyl, halomethyl. haloethyl, halobutyl, halopropyl, halopentyl, halohexyl, haloheptyl, haloctyl. halononyl, halodecyl, halophenyl, halobenzyl, haloxylyl, halothexyl, methylhalohexyl, halophenylmethyl, butylhalophenylmethyl, halovinyl, vinylhalomethyl or vinylhaloethyl. 35. Use according to claim 33 or 34 c ha ra cte rised in, that at least one R is a moiety derived from a polymer or copolymer, such as polyethylene, a polystyrene, a polyether or a polyester 36. Use according to claim 35 characterised in, that said polymer is silylated and/or halogenated. 37. Use according to any of the claims 33-36 chara cterised in, that X is Si. 38. Use according to any of the claims 33-37 cha racterised in, that the catalyst is a trialkylhalosilane. 39. Use according to claim 38 characterised in, that the trialkylhalosilane is a trialkylchlorosilane, trialkyliodosilane, trialkyl- bromosilane or trialkylfluorosilane. 40. Use according to claim 38 or 39 characterised in, that the catalyst is trimethylchlorosilane, triethylchlorosilane, tributylchlorosilane, tripropylchlorosilane, trimethyliodosilane, triethyliodosilane, tributyl- iodosilane, tripropyliodosilane, trimethylbromosilane, triethylbromosilane, tributylbromosilane, tripropylbromosilane, trimethylfluorosilane, triethyl- fluorosilane, tributylfluorosilane, tripropylfluorosilane.

Inventors: CRAMER FRIEDRICH, DABKOWSKI WOJCIECH, MICHALSKI JAN, CRAMER, FRIEDRICH, DABKOWSKI, WOJCIECH, MICHALSKI, JAN
Applicants: CRAMER FRIEDRICH, DABKOWSKI WOJCIECH, MICHALSKI JAN, PERSTORP AB, POLISH ACADEMY OF SCIENCE, CRAMER, FRIEDRICH, DABKOWSKI, WOJCIECH, MICHALSKI, JAN
Application year: 1997
Main classification: C07C7
IPC codes: B01J31, C07B61, C07F9, C07H1, C07H11, C07H19, C08F8