GENETIC CONTROL OF FRUIT RIPENING

Abstract: A method of modulating the ripening and/or senescence characteristics in plants of the genus Musa comprises transforming plants with one or more sequences obtainable from the deposited cDNA library having the accession number 40184, regenerating said plants and selecting from the population of transformants those plants having modulated and/or tissue senescence characteristics.

Description: GENETIC CONTROL OF FRUIT RIPENING This invention relates generally to the modification of a plant phenotype by the regulation of plant gene expression. More specifically it relates to the modulation of the ripening and/or tissue senescence characteristics and plants derived therefrom. One suitable application of the present invention is the modulation of ripening and/or senescence processes in plants of the genus Musa (referred to herein as banana). Two principal methods for the control of expression are known, viz.: overexpression and underexpression. Overexpression may be achieved by insertion of one or more than one extra copies of the selected gene. It is, however, not unknown for plants or their progeny. originally transformed with one or more than one extra copy of a nucleotide sequence, to exhibit the effects of underexpression as well as overexpression. For underexpression, often referred to as "gene silencing", there are two principle methods which are commonly referred to in the art as "antisense downregulation" and "sense downregulation (also referred to as "cosuppression"). Both of these methods lead to an inhibition of expression of the target gene. Other lesser used methods involve modification of the genetic control elements, the promoter and control sequences, to achieve greater or lesser expression of an inserted gene. There is no reason to doubt the operability of these methods: they are well- established, used routinely in laboratories around the world and products in which they have been used are on the market. Gene control by any of these methods requires the insertion of a selected gene or genes into plant material which can be regenerated into plants. This transformation process can be performed via a number of methods, for example: the Agrobacterium-mεdiatsd transformation method. In the microparticle bombardment method, microparticles of dense material, usually gold or tungsten, are fired at high velocity at the target cells where they penetrate the cells, opening an aperture in the cell wall through which DNA may enter. The DNA may be coated on to the microparticles or may be added to the culture medium. In microinjection. the DNA is inserted by injection into individual cells via an ultrafine hollow needle. Another method, viz. fibre-mediated transformation, applicable to both monocots and dicots, involves creating a suspension of the target cells in a liquid, adding microscopic needle-like material, such as silicon carbide or silicon nitride "whiskers", .and agitating so that the cells and whiskers collide and DNA present in the liquid enters the cell. In summary, then, the requirements for both sense and antisense technology are known and the methods by which the required sequences may be introduced are known. What remains, then is to identify genes whose regulation will be expected to have a desired effect, isolate them or isolate a fragment of sufficiently effective length, construct a chimeric gene in which the effective fragment is inserted between promoter and termination signals, and insert the construct into cells of the target plant species by transformation. Whole plants may then be regenerated from the transformed cells. Bananas are a globally important fruit crop. They are not only a popular dessert fruit, but represent a vital carbohydrate staple in the tropics with as many as 100 million people subsisting on bananas and plantains as their main energy source. The cultivated dessert banana is commonly triploid, parthenocarpic and belongs to the Musa AAA genome group, eg. Cavendish subtypes. Bananas are climacteric fruits and ripening is regulated by ethylene produced by the fruit and involves numerous biochemical changes including the conversion of starch to sugars, cell wall disassembly, synthesis of volatile compounds, changes in phenolic constituents and degradation of chlorophyll in the peel. The conversion of starch to sugars is particularly striking, where starch accounts for 20-25% of the fresh weight of the unripe fruit and depending on the genetic background, can be converted almost entirely to sugars. The triploid nature of the cultivated dessert banana crop has hampered conventional methods of breeding for improved characteristics. As a result of this an enormous pool of genetic resources for enhancing postharvest characteristics of the fruit has remained untapped. According to the present invention there is provided a method of modulating the ripening or tissue senescence process in pl.ants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID-Nos. 1 -57. regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics. The said polynucleotide may be obtained from the cDNA library having the NCIMB Accession Number 40814. Further according to the present invention is a method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60°C to 65°C in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics. The invention further provides a method as described above wherein the said polynucleotide is capable of modulating the production of pectate lyase and more specifically the polynucleotide comprises at least one of the sequences depicted in the sequence listings as SEQ-ID-Nos. 13-18. A preferred method for inserting the said polynucleotides into plant material according to the method of the present invention, may be selected from the group comprising the Agrobacteήum, microparticle bombardment, fibre mediated or direct insertion methods. The invention further provides plants, their progeny and seed and material obtained from said plants, produced according to the method of the present invention. It is preferred that the said plants, their progeny and seed and material obtained from said plants are derived from plants of the genus Musa. The present invention also provides a vector functional in plants comprising a promoter region which is operable in plant cells, at least one of the polynucleotide sequences described above and a transcription termination sequence. The promoter may be constitutive, developmentally regulated or switchable. In addition to this the promoter may also be tissue specific or organ specific. Further provided is a banana produced via the preceding method, having altered fruit characteristics when compared with a banana which is not transformed with at least one of the polynucleotide sequences described above. The present invention also provides an improved method of controlling plant pathogens compπsing the application of an anti-pathogenic agent to plants, characterised in that plants to which the said agent is applied, are plants according to the present invention. The gene sequences of the present invention may be synthesised ab initio, using the sequence data in the sequence listing provided herewith, or isolated from a library using the standard techniques know within the art. The sequences depicted in the sequence listing or parts thereof may also be used to create oligonucleotide probes for the purposes of isolating from the library those polynucleotides which are capable of producing the desired proteins. To assist the isolation of these polynucleotides we have deposited with the National Collection of Industrial & Marine Bacteπa, St. Machar Drive, Aberdeen, UK, a cDNA library of the banana peel ripening related genes. The library was deposited on 9th July 1996 and has the NCIMB Accession Number 40814. Thus, this invention is based on the identification of genes which encode proteins involved in banana ripening-related processes, specifically within banana pulp. DNA sequences according to the sequence listing or those sequences obtainable from the deposited library, may be used in the process of modifying the plant ripening characteπstics of plants and/or fruit. By virtue of this invention banana plants can be generated which, amongst other phenotypic modifications, may have one or more of the following fruit characteπstics: improved resistance to damage duπng harvest, packaging and transportation due to slowing of the πpening and over-πpenmg processes; longer shelf life and better storage characteπstics due to reduced activity of degradative pathways (e.g. cell wall hydrolysis): improved processing characteπstics due to changed activity of proteins/enzymes contributing to factors such as: viscosity, solids, pH, elasticity; improved flavour and aroma at the point of sale due to modification of the sugar/acid balance and other flavour and aroma components responsible for characteristics of the πpe fruit; modified colour due to changes m activity of enzymes involved in the pathways of pigment biosynthesis (e.g. lycopene, β-carotene. chalcones and anthocyamns), increased resistance to post-harvest pathogens such as fungi. The activity of the πpemng-related proteins may be either increased or reduced depending on the characteπstics desired for the modified plant part (fruit, leaf, flower, etc). The levels of protein may be increased: for example, by incorporation of additional genes. The additional genes may be designed to give either the same or different spatial and temporal patterns of expression m the fruit "Antisense" or "partial sense" or other techniques may be used to reduce the expression of ripening-related protein. The activity of each ripening-related protein or enzyme may be modified either individually or in combination with modification of the activity of one or more other ripening- related proteins/enzymes. In addition, the activities of the ripening-related proteins/enzymes may be modified in combination with modification of the activity of other enzymes involved in fruit ripening or related processes. DNA constructs according to the invention for gene silencing, may compπse a base sequence at least 10 bases (preferably at least 35 bases) in length for transcπption into RNA. There is no theoretical upper limit to the base sequence, it may be as long as the relevant mRNA produced by the cell but for convenience it will generally be found suitable to use sequences between 100 and 1000 bases in length. When using genomic DNA as the source of a base sequence for transcπption it is possible to use either intron or exon regions or a combination of both. As a source of the DNA base sequence for transcription, a suitable cDNA or genomic DNA or synthetic polynucleotide may be used. The isolation of suitable npening-related sequences is descπbed above; it is convenient to use DNA sequences derived from the ripening-related clones deposited at NCIMB in Aberdeen. Sequences coding for the whole. or substantially the whole, of the appropπate npening-related protein may thus be obtained Suitable lengths of this DNA sequence may be cut out for use by means of restπction enzymes. The preparation of such constructs is described in more detail below Constructs suitable for expression of the appropπate ripening-related sequence in banana cells, may be produced using a cDNA sequence selected from the deposited library having the NCIMB Accession Number 40814 or the gene sequence as found in the chromosome of the banana plant. Recombinant DNA constructs may be made using standard techniques. In this specification modulation means an increase or decrease of the desired effect More specifically ''modulation of the npening or tissue senescence process in plants of the genus Musa " means an increase or decrease in production of a πpemng related protein resulting from the method as descπbed above For example, where an increased πpemng related protein is desired, plants may be transformed according to the method as descπbed above and those plants exhibiting the desired effect may be selected from the population of trans formants. Furthermore, it may be desirable to provide a plant with modulated ripening or tissue senescence characteristics by increasing the production of one protein and decreasing the production of another protein in the same plant. For example, a banana fruit, modified using the present method, and having decreased levels of the enzyme pectate lyase would be beneficial because pulp softening would require a substantially longer time when compared with a control. In addition to this and by increasing the levels of another ripening related protein such as an antifungal protein in the same fruit using the present method, would complement the extended life of the banana pulp with increased resistance to disease. Increase in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing a protein and thereby increasing protein levels when compared with a control plant. Decrease in production of a ripening related protein includes inserting into plant material one or more copies of any of the polynucleotides described above, wherein the said polynucleotides must be capable of producing an mR A which is capable of interfering with endogenous mRNA to such a degree that the levels of translated protein are reduced when compared with a control plant. Ripening process of plants means the process of maturing or developing . Senescence means the progressive deterioration in function of cells, tissues, organs etc.. related to the period of time since that function commenced.. Control plant means a comparable plant used for the purposes of determining modulation of the πpening or tissue senescence process effect in plants. Specifically, in plant transformations the control pl∑int is usually of the same species and variety as the material used before the transformation process and is grown in the same conditions, (usually with the transformant selection step modified in some way on the part of the control plant), as the transformed plants. More specifically the control plant may comprise an untransformed control plant or a transformed control plant providing it has not already been transformed with the same polynucleotide sequence as the plant material to be transformed. "Plant material" includes plant ceils and any other type of plant regenerable material. The following examples further illustrate the invention but are not to be construed to limit the scope thereof: TABLE 1. Is a list of clones isolated from banana pulp and the corresponding sequence identity number .as provided in the sequence listing herein. The table also illustrates the approximate clone size, the percentage identity and, where relevant, nucleotide similarity with published sequences, based on the results obtained from comparisons with the EMBL sequence database. The table also provides, where relevant, the gene identity of those published sequences and their database accession numbers. FIGURE 1. Plant transformation vector pUN. containing the UBI polyubiquitin promoter. FIGURE 2. Plant transformation vector pSHYN, containing hygromycin resistance gene for selection of transformed plants. FIGURE 3. Plant transformation vector pAN. containing the banana ACC oxidase promoter. EXAMPLE 1 Construction of a cDNA library of ripening genes 1.1 Isolation of messenger RNA Total RNA was isolated from ripening (24 hours after ethylene treatment) banana pulp (Musa acuminata cv. Grand Nain) as described by Chang et al. Plant Molecular Biology Reporter. Vol. 1 1(2) 113-1 16 (1993). Messenger RNA was isolated from total RNA by Oligo(dT)- cellulose chromatography according to Bantle et al., Analytical Biochemistry 72, 413-427 (1976). 1.2 Synthesis of cDN A and Cloning into Vector The first and second strands of the cDNAs were svnthesised from the messenger RNAs using a commercial cDNA synthesis kit (Catalog No. 200450, ZAP Express™ Gold Cloning kit. Stratagene Ltd. Cambridge. Ca bs, UK). Double stranded cDNAs were cloned into the ZAP Express™ vector, packaged, mixed with plating bacteria to determine titre and for library screening, following instructions of the suppliers protocol. 1.3 Screening of the cDNA library from banana pulp. The unamplified cDNA library from ripening banana pulp was differentially screened using cDNA from unripe and ripening banana peel tissue. A proportion of the library was plated individually at low density and duplicate plaque lifts made onto Hybond N nylon filters (Amersham) according to t e manufacturer's instructions. One filter was hybridised to dCTP radiolabelled cDNA from green fruit and the duplicate filter hybridised to dCTP radiolabelled cDNA from ripening fruit. Hybridization's were at high stringency. Plaques hybridising preferentially with ripening or green radiolabelled cDNA were picked and re- plated for a second round of selection by differential screening. These clones were numbered as ripening up- or down-regulated peel clones. The clones were in-vivo excised from the ZAP express™ vector into the pBK-CMV phagemid vector using the E.xAssist™ interference-resistant helper phage, following instructions from manufacturers protocol. 1.4 Characterisation of the ripening pulp cDNA library and the ripening-related clones. The ripening cDNA library from pulp tissue were prepared with an efficiency of 3.2 x 10 5 plaque-forming units per microgram of cDNA. The sizes of the inserts in the peel library was 0.4 - 6.7 Kb with a mean size insert of 1.47 Kb. From the 250 plaques used in the first screen, 73 putative ripening-related clones were obtained. These 73 clones were partially sequenced using the ABI PRISMTM Dye Terminator Cycle Sequencing Ready Reaction kit with AmpliTaq ® DNA polymerise (Applied Biosystems, Warrington, Cheshire, UK) with forward pπmers specific for the pBK- CMV vector. From these, the following ripening-related clones were selected. Comparisons of these sequences in the EMBL database using GCG ('Wisconsing') software has identified homologies for the clones listed in TABLE 1 below. EXAMPLE 2 Construction of truncated sense RNA vectors with the maize polyubiquitin promoter. A vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is svnthesised by polymerase chain reaction using synthetic pπmers incorporating BamHI restπction sites suitable for clomng between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology, 18:675-689) and a nopaline synthase 3 'end termination sequences in the vector pUN (Fig. 1.). The truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase. pSHYN contains hygromycin resistance gene for selection of transformed plants. After synthesis of the vector, the structure and orientation of the sequences are confirmed by DNA sequence analysis. EXAMPLE 3 Construction of truncated sense RNA vectors with a fruit enhanced promoter. The 1386bp Hindlll fragment containing the banana ACC oxidase promoter (UK. Application No. 9607700.3) is cloned the Hindlll site in pMSC2 (Fig. 3.) to give the vector pAN. A vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 57. This fragment is synthesised by polymerase chain reaction using synthetic primers incorporating BamHI restriction sites suitable for cloning between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology, 18:675-689) and a nopaline synthase 3'end termination sequences in the vector pAN. The truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase. pSHYN contains hygromycin resistance gene for selection of transformed plants. After synthesis of the vector, the structure and orientation of the sequences are confirmed by DNA sequence analysis. EXAMPLE 4 Construction of an over-expression vector with the maize polyubiquitin promoter. The complete sequence of a ripening -related cDNA containing a full open-reading frame is inserted into the vectors described in EXAMPLE 2. EXAMPLE 5 Construction of an over-expression vector with a fruit enhanced promoter. The complete sequence of a ripening -related cDN A containing a full open-reading frame is inserted into the vectors described in EXAMPLE 3. EXAMPLE 6 Generation of transformed Musa plants Transformed Musa plants containing the vectors are produced by the method described in Sagi et al. (1995) Biotechnology. Vol. 13 pp 481-485. Regenerated transformed plants are identified by their ability to grow on hygromycin and grown to maturity. Ripening fruit are analysed for a modulation in their ripening related or senescence characteristics. Other suitable transformation methods for banana are described in Sagi et al. ( 1994) Plant Cell Reports. Vol. 13. pp 262-266. and May et al. (1995) Biotechnology. Vol. 13 pp 486- 492. TABLE 1 Sequence Croup Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp SEQ-ID-NO- 1 Pulp U-U9 Isoflavone Reductase 61 .6 714 Potato (Solanum Upregulated tuberosum), X92075 c SEQ-ID-NO-2 Pulp U-U 17 Isoflavone Reductase 62.9 770 Potato (Solanum CD C/> Upregulated tuberosum), X92075 H SEQ-ID-NO-3 Pulp U-U66 1.1 Isoflavonoid 60.0 722 Arabidopsis thailiana, H C Upregulated Reductase Z49777 H m SEQ-1D-NO-4 Pulp U-U 104 Isoflavonoid 60.6 696 Arabidopsis thailiana cn x Upregulated Reductase Z49777 m SEQ -ID-NO-5 Pulp U-U I 3 1.1 Beta- 1 ,3-Glucanase 58.5 585 Soybean (Glycine max), 5 Upregulated A26451 c SEQ -ID-NO-6 Pulp U-U 136 1.3 Beta-1 ,3-Glucanase 59.8 800 Barley (Hordeum vulgare fn Upregulated M91814 SEQ -ID-NO-7 Pulp U-U21 1.8 Transcriptional 54.3 31 1 Zea mays, LI 3454 σ> Upregulated Activator Sequence Croup Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp SEQ -I -NO-8 Pulp U-U31 0.55 S- 67.0 467 Orchid (Phalaenopsis sp.), I Ipregulated adenosylhomocysteine X79905 Hydrolase SEQ -ID-NO-9 Pulp U-U 131 1.8 S-adenosyl L- 79.8 699 Wlieat (Triticum aestivum cn c Upregulated homocysteine L 1 1872 CD Hydrolase cn H SF.Q -ID-NO- 10 Pulp U-U32 0.8 Beta-amylase 54.7 854 Zea mays, Z25871 c H Upregulated H m SEQ -ID-NO- 1 1 Pulp U-U55 0.8 O-methyl transferases 60.1 223 Zea mays, L 14063 cn Upregulated -π m m H SEQ -1D-NO-12 Pulp U-U72 0.8 O-methyl transferase 60.6 226 Zea mays, LI 4063 Upregulated c r m* SEQ -ID-NO-13 Pulp U-U68 1.8 Pectate Lyase 57.9 394 Lilium longifloritm, Z1732 t Upregulated SEQ -ID-NO- 14 Pulp U-U69 1.7 Pectate Lyase 66.1 516 Zea mays, L20140 Upregulated SEQ -ID-NO- 15 Pulp U-U84 1.1 Pectate Lyase 65.8 736 Lycopersicon esculenlum, Upregulated X55193 SEQ -ID-NO- 16 Pulp U-U89 1.6 Pectate Lyase 54.8 354 Nicoliana tabacum, X671 Upregulated Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp SEQ -1D-N0- 17 Pulp U-U1 15 1.5 Pectate Lyase 67.3 508 Zea mays, L20140 Upregulated SEQ -ID-NO- 18 Pulp U-U 1 17 1.7 Pectate Lyase 55.0 333 Lilium longiflυrum, Z 173 Upregulated J) C SEQ -ID-NO- 19 Pulp U-U80 0.6 Vicilin-like storage 53.7 387 Arabidopsis thailiana, CD Upregulated protein T13642 H SEQ -ID-NO-20 Pulp U-U90 1.8 Glutamate 72.5 735 Petunia hybrida, L 16797 H C Upregulated Descarboxylase H m SEQ -ID-NO-21 Pulp U-U92 1.8 Glutamate 71.8 740 Arabidopsis thailiana, n X Upregulated Descarboxylase U 10034 m SEQ -ID-NO-22 Pulp U-U91 1.1 Aconitase 76.4 766 Pumpkin (Cucurbita sp.), Upregulated D29629 3D C SEQ -ID-NO-23 Pulp U-U96 0.9 Express Sequence 69.0 452 Arabidopsis thailiana, r- m Upregulated Tag. H36910 ro SEQ -ID-NO-24 Pulp U-U 103 2 Cell Wall Invertase 66.0 567 Zea mays, V 17695 Upregulated SEQ -ID-NO-25 Pulp U-U93 3.5 Heat Shock Protein 76.4 71 1 Lycopersicon esculentum, Upregulated cognate X54030 SEQ -ID-NO-26 Pulp U-U 125 1.8 Heat Shock Protein 71.0 662 Spinacia oleracca, L2624 Upregulated SEQ -ID-NO-27 Pulp U-U105 0.9 Hexaubiquitin Protein 64.1 237 Sunflower (Helianthus Upregulated annuus), X57004 Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp SEQ -ID-NO-28 Pulp U-U1 16 0.8 Polyubiquitin 81.0 625 Rice (Oryza sativa), X76 Upregulated SEQ -ID-NO-29 Pulp U-U 120 0.9 Extensin 56.9 576 Almond (Prunus Upregulated amygdalus), X6 l\ % ω c SEQ -1D-NO-30 Pulp U-U 126 1.2 Basic Chitinase 63.2 506 Cowpea (Vigna 09 Upregulated unguiculata), X88801 V) -i SEQ -1D-NO-31 Pulp U-U129 1.3 Beta-glucosidase 60.9 517 White clover (Trifolium H C Upregulated repens), X56733 H m SEQ -ID-NO-32 Pulp U-U130 1.3 Chlorophyll aft 52.0 415 Rice (Oryza saliva), D006 cn Upregulated binding protein x m SEQ -ID-NO-33 Pulp U-U43 2.5 Fruit sp pp involved in 53.8 396 Tomato (Lycopersicon m H Upregulated maturation esculentum), XI 3743 SEQ -ID-NO-34 Pulp U-U70 0.6 Root specific protein 63.6 420 Rice (Oryza sativa), L272 c Upregulated m SEQ -ID-NO-35 Pulp U-U16 0.6 - - - No published sequence σ> Upregulated similarity SEQ -ID-NO-36 Pulp U-U30 0.8 - • - - Upregulated SEQ -ID-NO-37 Pulp U-U40 10 - - - - Upregulated SEQ -ID-NO-38 Pulp U-U 108 1.1 - - - - Upregulated Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp SEQ -ID-NO-39 Pulp U-U 128 2.2 - - - - Upregulated SEQ -ID-NO-40 Pulp U-D4 1.3 Granule Bond Starch 62.3 621 Cassava (Manihol Down Synthase esculenta), X74160 c regulated CD α> SEQ -ID-NO-41 Pulp U-D10 1.3 Starch Synthase 68.8 506 Pea (Pisum sativum), H Down X88789 H regulated m SEQ -ID-NO-42 Pulp U-D13 1.3 Starch Synthase 67.4 454 Pea (Pisum sativum), (/> Down X88789 x m regulated m H SEQ -ID-NO-43 Pulp U-D66 1.3 Granule Bond Starch 64.1 669 Cassava (Manihol Down Synthase esculenta), X74160 c r- regulated m ι SEQ -ID-NO-44 Pulp U-Dl l l 1.3 Starch Synthase 66.4 655 Cassava (Manihol σ> Down esculenta), X74160 regulated SEQ -ID-NO-45 Pulp U-D1 12 2.3 Granule Bond Starch 68.4 196 Potato (Solanum Down Synthase tuberosum), X58453 regulated Sequence Group Clone Size Published Sequence % Identity Sequence Published Sequences Identity no. Kb gene Identity Bp SEQ -ID-NO-53 Pulp U-D65 0.9 Extensin 56.7 556 Cowpea (Vigna Down unguiculatά), X91836 regulated SEQ -ID-NO-54 Pulp U-D84 0.8 Extensin 58.3 492 Cowpea (Vigna CΛ Down unguiculata), X91836 C CD regulated 09 • H SEQ -ID-NO-55 Pulp U-D75 1.2 Wali 7 74.4 703 Wheat (Triticum aestivum H C Down L28008 H m regulated cn SEQ -ID-NO-56 Pulp U-D83 1.2 Wali 7 72.8 743 Wheat (Triticum aestivum x Down L28008 regulated 31 SEQ -1D-NO-57 Pulp U-D64 2.3 C r-. Down m regulated SEQUENCE LISTING (1) GENERAL INFORMATION: (i> APPLICANT: (A) NAME: ZENECA LIMITED (B) STREET: 15 STANHOPE GATE (C) CITY: LONDON (D) STATE: LONDON (E) COUNTRY: UNITED KINGDOM (F) POSTAL CODE (ZIP): W1Y 6LN (ii) TITLE OF INVENTION: GENETIC CONTROL OF FRUIT RIPENING (iii) NUMBER OF SEQUENCES: 57 (iv) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.30 (EPO) (v) CURRENT APPLICATION DATA: APPLICATION NUMBER: GB PPD (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 785 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U9 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: CGGCACGAGG AAAAACTANG TGAGAANGAG ATAATCGTTG ACCGAGGNAG AGAATGGCGA 60 GCGAGAAGAG CAAAATCCTG ATCATCGGGG GCACCGGGTA CATCGGCAAG TTCATCGTGT 120 TTGCGAGCGC CAGGTTAGGT AACCCTACCT TCGCTCTCGT CCGGAGCACC ACCGCCCCCG 180 CCGGCCAACC CGAGAAGGCC AAGCTCCTGA GCGACTTCCA GGCCGCCGGC GTCACCCTCG 240 TCCAGGGGGA TATNTATAAC CACGAGAGTC TGGTTAAGGC GATCAAGCTG GTGGATGTGG 300 TCATCTCCCC CGTCGGCTTC GGGCANCTGA NTGATCAGAC CAAGATCATC GACGCCATCN 360 AANAAGCCGG AGGACACATC AAGAGGTACC TTCCATCGGA GTTTGGCAAC GACGTANACC 420 GAAGCCATGC TGTGGAGCCA GCAAAGTCTA CCTTTGTCGT CAAGCAACAA ATCANAAGGG 480 CTGTTGAGGC ATCGGGTNTC CCTTACACCT TTGTATCTTC C.AACTTTCTT CGGTGGGTNT 540 TTCCTCCCGG TATTATGACA GGCAGGAGCC ACTGGTCCTC CCACGGACAA GGTTGTCATC 600 TTAGGTNACG GGAACNCAAA ACGATCTTCT CNATGAANAC GACTTCCGGA CATCCCATTT 660 ANTCTTGGAT GATCCATAAC CTGAACCAGG TTCTATTTCT TAAAACTTCC NCCACTTCTT 720 NTCTCTTANC ANCTCNTTTC CCTCTTGGGA AAAAAATGTC NNCTANACTT CNAAAAGGGT 780 TTANT 785 (2) INFORMATION FOR SEQ ID NO: 2: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (11) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U17 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: CGCGCACGAG GAAGAAAACT AGGTGAGAAN GAGATAATCG TTGACCGAGG NAGAGAATGG 60 CGAGCGAGAA GAGCAAAATC CTGATCATCG GGGGCACCGG GTACATCGGC AAGTTCATCG 120 TGTTTGCGAG CGCCAGGTTA GGTAACCCTA CCTTCGCTCT CGTCCGGAGC ACCACCGCCC 180 CCGCCGGCCA ACCCGAGAAG GCCAAGCTCC TGAGCGACTT CCAGGCCGCC GGCGTCACCC 240 TCGTCCAGGG GGATATATAT AACCACGAGA GTCTGGTTAA GGCGATCAAG CTGGTGGATG 300 TGGTCATCTC CCCCGTCGGC TTCGGGCANC TGANTGATCA GACCAAGATC ATCGACGCCA 360 TCAAAGAAGC CGGAGGACAC ATCAAGAGGT ACCTTCCATC GGAGTTTGGC AACGACGTAN 420 ACCGAAGCCA TGCTGTGGAG CCAGCAAAGT CGACCTTTGT CGTCAAGCAA CAAATCANAA 480 GGGCTGTTGA GGCATCGGGT ATCCCTTACA CCTTTGTATC TTCCAACTTC TTCGGTGGGT 540 NTTTCCTCCC GGTATTANGA CAGGCAGGAG CCACTGGTCC TCCCACGGAC AAGGTTGTCA 600 TCTTANGTGA CGGGAACACA AAAGCGATCT TTCTCAATGA ANACACATCC GGACNTNCAC 660 NATTAAAGCA GTGGATGATC CGAAAACCTG AACANGTTCT ATATCTGAAA CCTTCCGCCA 720 CTCTTGTCTC ATNACAACTC ATTTCCCTCT GGGAAAAAAA NGTCNGCAAA ACTCCNAAAA 79C GGTCTACTTC CCGGAAGAAA AATCTGAANC ANA 813 (2) INFORMATION FOR SEQ ID NO: 3: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 746 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (li) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U66 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: GGCACGAGGG GAAAA.GGAGA TNATCGTTGA CCGAGGAGAG AATGGCGAGC GAGAAGAGCA 60 AGATCCTGAT CATCGGGGGC ACGGGGTACA TCGGCAAGTT CATCGTGTTT GCGAGCGCCA 120 GGTTAGGTAA CCCTACCTTC GCTCTCGTCC GGAGCACCAC CGCCCCCGCC GGCCAACCCG 130 AGAAGGGCAA GCTCCTGAGC GACTTCCAGG CCGCCGGCGT CACCCTGGTC CAGGGGGATA 240 TATATAACCA CNAGAGTCTG GTTAAGGCGA TCAAGCTGGT GGATGTGGTC ATCTCCCCCG 300 TCGGCTTCGG GCAGCTGAGT GATCAGACTA AGATCATCGA CGCCATCAAA GAAAGCCGGA 360 GGACACATCA AGAGGTACCT TCCATCGGGA GTTTGGCAAC GACGTANACC GAAGCCATGC 420 TGTGGAAGCC ANCAAAGTCG ACCTTTGTCG TCAAGCAACA AATTANAAGG GCTGTTGAGG 480 CATCGGGGAT CCCTTACACC TTTGTTATCT TCCAACTTCT TCGGTGGGTA TNTCCTCCCC 540 GTATTGGGAC AGGCANGAAC CACTGGTCCT CCCCACGGAC AAGGTTGTCN TCTTAGGTGA 600 ACGGGAACAC CAANGCGATC TTTCTCAATG AAAGACAACT CGGGACATNC CCNATTTAAC 660 CANTGGATGA TCCNANAACC TGAACAAGGT CTATTTCTGA AAACTTCNCC ATCTTCTTTT 720 TCTCATAACG AACCCNTTTN CCTCTT 746 (2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 795 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U104 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: GGCACGAGGA AANGAGATAA TCGTTGACCG AGGCAGAGAA TGGCGAGCGA GAAGAGCAAA 60 ATCCTGATCA TCGGGGGCAC CGGGTACATC GGCAAGTTCA TCGTGTTTGC GAGCGCCAGG 120 TTAGGTAACC CTACCTTCGC TCTCGTCCGG AGCACCACCG CCCCCGCCGG CCAACCCGAG 180 AAGGCCAAGC TCCTGAGCGA CTTCCAGGCC GCCGGCGTCA CCCTCGTCCA GGGGGATATA 240 TATAACCACG AGAGTCTGGT TAAGGCGATC AAGCTGGTGG ATGTGGTCAT CTCCCCCGTC 300 GGCTTCGGGC AGCTGAGTGA TCAGACCAAG ATCATCGACG CCATCAAAGA AGCCGGAGGA 360 CACATCAAGA GGTACCTTCC ATCGGGAGTT TGGCAACGAC GTTANACCGA AGCCATGCTG 420 TGGAGCCAGC AAAGTCNACC TTTGTCGTCA AGCAACAAAT CANAAGGGCT GTTGANGCAT 480 CGGGTNTCCC TTACACCTTT GTATCTTCCA ACTTCTTCGG TGGGTATTTC CTCCCGGTAT 540 TANGACAGGC AGGACCACTG GTCCTCCCCA CNGACAAGGT TGTCNTCTTA GGTGACNGGA 600 ACACAAAANC ATCTTTCTCN ATGAAGACAA CTCCGGACAT ACNCNATTAA AGCNGTGGAT 660 GATCCCAAAA CCTGAACAAG TTCCTATNTC TTGAAAACTT TCCCGCCCAA CCATCCTTTN 720 GTTCTCCCNT TAAACCNAAC CTCCATTTTC CCCTCCTTGG GGAAAAAAAA AAGGTCCGGC " 90 NAANAACTTC CNAAA 795 (2) INFORMATION FOR SEQ ID NO: 5: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 797 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: CDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U13 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: CGGCACGAGG NAGAACCTTT TGACAGAGTT GTTGTCATGG CAACAAAAGC TTCTCTCTCC 60 ATAAAAGGCT TTGCCTTGCT GGTTTCAGTC CTTGTAGCAG TTCCAACAAG AGTGCAATCG 120 ATTGGTGTCT GCTACGGCAT GCTCGGCAAC AATCTTCCCC CGCCCAGCGA GGTGGTCAGT 180 CTCTACAAAT CCAACAACAT CGCGAGGATG AGACTCTACG ATCCAAACCA AGCCGCCCTG 240 CAAGCCCTCA GGAACTCCAA CATCCAAGTC CTGTTGGATG TCCCCCGATC CGACGTGCAG 300 TCACTGGCCT CCAATCCTTC GGCCGCCGGC GACTGGATCC GGAGGAACGT CGTCGCCTAC 360 TGGCCCAGCG TCTCCTTTCG ATACATAGCT GTCGGAAACG AGCTGATCCC CGGATCGGAT 420 CTGGCGCAGT ACATCCTCCC CGCCATGCGC AACATCTACA ATGCTTTGTC CTCGGCTGGC 480 CTGCAAAACC AGATCAAGGT CTCGACCGCG GTCGACACGG GCGTCCTCGG CACGTCCTAC 540 CCTCCCTCCG CCGGCGCCTT CTCCTCCGCC GCCCAGGCGT TACCTGANCC CCATCGTGCA 600 GTTCTTGGCG ANTAACGGAA CGCCGCTCCT GGTCAATGTG TACCTTATTT TAACTACACC 660 GGCAACCCGG GANAGATCTC GCTGCCTACN CCCTGTTCAC GGCCNCGGGG TCTCNTGCAG 720 GATGGGCGAA TTCCNCTATC ANAANCTGTT CANTCCATCT TCNAAACCGG TCTTCCCGGG 780 CTGGAAAAAA TTGGAAG 797 (2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 792 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U136 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: GGCACGAGGA GAACCCTTTT GACAGAGTTG TTGTCATGGC AACAAAAGCT TCTCTCTCCA 60 TAAAAGGCTT TGCCTTGCTG GTTTCAGTCC TTGTAGCAGT TCCAACAAGA GTGCAATCGA 120 TTGGTGTCTG CTACGGAATG CTCGGCAACA ATCTTCCCCC GCCCAGCGAG GTGGTCAGTC 180 TCTACAAATC CAACAACATC GCGAGGATGA GACTCTACGA TCCAAACCAG GCCGCCCTGC 240 AAGCCCTCAG GAACTCCAAC ATCCAAGTCC TGTTGGATGT CCCCCGATCC GACGTGCAGT 30C CACTGGCCTC CAATCCTTCG GCCGCCGGCG ACTGGATCCG GAGGGAACGT CGTCGCCTAC 360 TGGCCCAGCG TCTCCTTTCG ATACATAGCT GTCGGAAACG ANCTGATCCC CGGATCGGAT 420 CTGGCGCAGT ACATCCTCCC CGCCATGCGC AACATCTACA ATGCTTTGTC CTCGGCTGGC 480 CTGCAAAACC AGATCAAGGT CTCCGACCGC GGTCGACACG GGCGTCCTCG GCACGTCCTA 540 CCCTCCCTCC CGCCGGNGCC TTCTCCTCCG CCGCCCAAGC GTNCTGANCC CCATCGTGCA 600 NTTCTTTGGC GAAATAACGG ANCGCCGCTC CCNGGTCCAT GTTTTTCCCT TATTTTAACT 660 ACNCCGGCAA CCCNGGAAAG ATTTCGCTGC CCTTACGCCC CCTGGTTTCC NNGGGGCTTC 720 CCGGGCGTTT CTTCCTTTGC CAGGGANTNG GGGCGAATTC CCNNCTTTTC CANAAACCTT 780 GTTCCAACNC CC 792 (2) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 855 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U21 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: CGGCACGAGT CTCTCTCTGT CTCTCCGTCG TCTCGTTGTC TGTTCGTTAG GGCTTGCGAT 60 CGCCACCGGT CGCGAGGGTT GGAGCCATGG AATTCTTGCG GTTTGGGGCT GAGGTGGCGG 120 CAGGAGAGGA AGCCGCGACG GGGTACTGGA TGCGATGGCA GACGCTCGTC TGCGCTCTGA 180 TCGTCGTCGC CCCGGCGGTC GCCGCGGTGG TTGTCGCGGC GCGGGCGCCG GCGCCGGCGC 240 GACCGCTCAG GGCCGTCGAT CTCTGGGCGC CGTGCTGGGC CGGGATGCAC CCGGCCTGGC 300 TTCTCGCGTA CCGCGGCTTC NTGTTCTTGG CCATGGCGTG GCTACTCTTC CANATGATTC 360 TGTTTCGCGG ATTCTCCGCG TTCTACTTCT ATACTCAGTG GACGTTTGCC TTAGTCATTG 420 TCTATTTTGC GATCGCAACC ATTATATCCG CCCATGGCTG CTGGCTTTAC TCAAAGAGAA 480 GTATTATGCC AGATCAGGAG GTCAACAGAT TCCTAAATGG TGGTTTTGAG CANAATAGTC 540 CTATGACTCT ACCTTTGAGG ACCAACAAAA ATATGAATGT TATAAGATTG CAAAGCTATC 600 NTGAACAGGA NGCTGATGAA NAAAAANCTG GATTTTGGGG TCCTGCTATG CNACTNGTCT 660 ATCAAAACCA TTGCANGTGC TGTAATTGTT GAAANACATT GTATTTTGGG NTCNTNCTAA 720 TACCATATTT GTCTTCTATA AATTTCAGGC TAAATGCTAT AATGGGCTGC ATGCATTCTC 780 TTAATGCTGT GTTTCTTCNA ATCCAACACT TTTCTCAATA ACCTGCCATT CCCNTNTTTC 840 NAATGGCATT TTTGC 855 (2) INFORMATION FOR SEQ ID NO: 8: Ii) SEQUENCE CHARACTERISTICS: (A) LENGTH: 722 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U31 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: CGGCACGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA GAGAGAGAGA 60 GAGAGAGAGC CTAGACCGGC ATCNTCGTGC TTGCCGAGGG GCGGCTTATG ANTCTTGGTT 120 GTGCCACTGG GCATCCCAGC TTCGTCNTGT CCTGCTCCTT CACCANCCAN GTGATNGCAC 180 NACTGGANTT ATGGANGGAA ANGGCGACCG GCNNGTACGA NAAGAANGTC TATGTGCTGC 240 CCANGCNTCT GGATGAGAGG GTGGCNGCGC TCCNCCTCGG CAAGCTGGGT GCCNCGCTCA 300 CCNAGCTTAC NCCNTCGCGG GCTGATTACA TTAGCGTCCC GATCGAGGGA CCCTACANGC 360 CTGCTCACTA CNGGTATTNG GGTTGCTTNT GCNAGAGACG ATGATNATAN NTCGGAGCAC 420 TGGCNTTTTC GACTANNAGT TTGACCGATG GCTATGTTCG TTTTGCTTTT CACCTTTTGT 480 CTTCCCATCT TTGCTGGTTC ACCTATGGAC GTTTGTTCCA TTTGGATGTN NTGAGAAATG 540 CTGATGGCAT TTTCGGAAAN AAAAANATNT AAAATCNCGA GAGTTCTTCT ANAGCGGCCG 600 CGGGCCCNTC NNTTTTCCCC CGGGTGGGGT ACCANGTTTG TTGTNCCCNT TCCCCCTNTT 660 TGAGTCCTTT TACTNCCCCT GGCCGTCGTT TTATACNTCG TTGACTGGGA ANACCCTGCC 720 NT 722 (2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 793 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U131 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: GGCACGAGCC TTGCCCAACT CCTTCGATTC GTTCCAACGA TCGGAGAATG GCGCTGCTGG 60 TGGAGAANAC GTCGACGGGA CGCGAGTACA AGGTGAAGGA CCTTTCTCAG GCTGACTTCG 120 GCCGCCTCGA GATCGAGCTG GCGGAGGTGG AGATGCCGGG CCTCATGGCG TGCCGCGCCG 180 AGTTCGGGCC CGCCAAACCT TTCGCCGGCG CCCGCATCTC CGGCTCCCTC CACATGACCA 240 TCCAGACCGC CGTCCTCATC GAGACCCTCA CCGCCCTCGG TGCTGAAGTC CGGTGGTGCT 300 CCTGCAACAT CTTCTCCACC CAGGACCACG CCGCTGCCGC CATTGCCCGC GACTCCGCCG 360 CCGTCTTCGC CTGGAAGGGA GAGACCCTCG CCGAGTACTG GTGGTGCACC GAGCGATGCC 420 TCGACTGGGG CCCCAACGGT GGCCCCGACC TCATCGTCGA CAAAGGGGGT GACGCCACTC 480 TCCTCATCCA CGAAGGCGTC AAGGCTGANG AAGANTACGA AAAAGACCGG CAAGCTGCCC 540 GATCCGGCCT CCACGGAAAA CGCCGAATTC CAAATCGTGC TGGGGATTAT CCGCNANCGG 600 CTCCAGGTCN AACCCCNAAA ATTACCGCCC AGATNAAAGA ACCCTCCTCC GCTTTTTGAA 660 GAAAACANCN CTGGCGTTCA NCGAATCTAC CANATGCCAG GGCCANCNGG GGCCCCTTGC 720 TTCTTTCCCC CCNCCCANTC NAACNTTCCN ACGAATTCCC GTTTTACCCN AAAAACCAAN 780 TTTTGAACAA CCT 793 (2) INFORMATION FOR SEQ ID NO: 10: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 847 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U32 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: CGGCACGAGG CGACGCCGGC CTTCGCCTCC GCGTCTCCCT CCACCTCCAC TGCCACCGCC 60 GCCCTCGCCT CCCCCTCCCC AAATCGGTCG ACTCCGCCGC CGCTACAGAC CCCGACATCC 120 TATTCACTGA CCGCGCCGGC CGCCGCCGTG CCGACTGCCT CTCCTTCGCT GTCGACGACC 180 TCCCTGTCCT CGACGGCAGG ACCCCGATGG AAGCCTACGA GGAGTTCTTC CGGAGCTTCC 240 GCCTCGCCTT CGCTGACTTC TTTGGTTCCG TCATTACGGA TATCACGATC GGTCTCGGGC 300 CAAATGGCGA GCTCCGTTAC CCTTCGTTTC CTCCCACTGG AAGCAATCGT TTCACCGGTG 360 TAGGAGAATT CCAGTGTTAT GACAAATACA TGCTTGCCGA TCTCAAACGA CACGCGGAGG 420 AAACCGGCAG CCCATTATGG GGCCTCTCTG GTCCTCACGA TGCCCCTGGG TACAACCAGT 480 CTCCGGACTT CGGCAACTTT TTCAAGGACC ATGGCGGCTC CTGGGANACA CCCTACGGGC 540 AATTCTTCCT CTCCTGGTAC ACCGGCAAGC TCCTGTCTCA CGGTGACGGC CTGCTCTCGG 600 TCGCGTCAGA AGTATTGGTG ATTTGCCTGT CGCGCTCTCG GGCAAGGTTC CACTTTCTGC 660 ATTTGTTGGC AACGAAACTC GGTCGCGCCC GTCTTANCTT GAAGGCCGGG TTCTNTNACA 720 CTTGACGGNA NAANAACNGG TTTCAAGGAT GTTGGCTAAN ATCTCCCGAA ANCNTCCTGC 780 ANCATGATTC ATCCCGGGCN TGGACTTCTC NGAAGGGGAA CANTCCTCAG GGTGTTCCGG 840 TCTGCCC 847 (2) INFORMATION FOR SEQ ID NC : 11: u) SEQUENCE CHARACTERISTICS: (A) LENGTH: 822 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (11) MOLECULE TYPE: cDNA (vu) IMMEDIATE SOURCE: (B) CLONE: U-U55 (xi ) SEQUENCE DESCRIPTION: SEQ ID NO: 11: GGCACGAGGC ATGGAGAACC CTGTTGCGAT GGGCATCATG GAGGAGCTTG AGGCCCAGAG 60 CGAGGTGTGG AACCACATTT TTAGATTCAT CACCTCCATG TCCGTCAAGT GCGCGGTGGA 120 GCTCAGGGTC CCCGACGCCA TCCACGCCCA CGGCGGCAAC GCAACTCTCC CTCAGCTGGC 180 CGCCGCCCTC AGCCTCCCTC CCGCCAAGCT CGCCGACCTC CGACGCCTCA TGCGCATGCT 240 GGTCCACGCC GGCTGCTTCG CCAAGCAGGA AGACNACGTG TACGCCCTCA CGCCATGGTC 300 GAGGCTCCTG NTGAGCTCCG AGCACACCGC GGTAGCCCCG TTCGTGGTGT GGATGCTCCA 360 CCCGCTCATG GTGCAGTCGT GGCACTCGCT GGGGGCGTGG TTCCACGGGA GGGCGCCCAC 420 CCCCTTCGCC GCAACCCACG GGAAAGGGGA TCTTCGAGAC NACACNCNAA CAGCCGGGGT 480 TCGCGGCCGT TCTTCANCNA NGCNATGGCG AACGACTGTC GGCTGGTGGG AAAGGTGTTG 540 GTAAANAACA TNCGGATGTG TTGGANGGAN CNCNGGTCCA TNGTGGAAGT TGGTGGCGCA 600 CCGGCACCCT TTTNGGCCAT TGTTGGCCGA AGGCTTTCCC GCCAACATNA AATNGCACCG 660 TTCTCCNANT TTGCCCCACT TTTCTGGCCN CNGCCGCCGG GGCCCCCNCN NACCNAAACA 720 ACTTGGATGT TTTTNGGGGG AANACATNTT CCAACATTTT ACANCCGCNC GACNTTTTTT 780 ACNCAATTGG TTCCNGGCNC NAATGGAAAT ANTNCTGNAT TT 822 (2) INFORMATION FOR SEQ ID NO: 12: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 743 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U72 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: GGCACGAAGA GCCCTGTACC GGANAGCATG GAGAACCCTG TTGCGGTGGG CATCATGGAG 60 GAGCTTGAGG CCCAGAGCGA GGTGTGGAAC CACATTTT A GATTCATCAC CTCCATGTCC 120 GTCAAGTGCG CGGTGGAGCT CAGGGTCCCC GACGCCATCC ACGCCCACGG CGGCAACGCA 180 ACTCTCCCTC AGCTGGCCGC CGCCGTCAGC CTCCCTCCCG CCAAGCTCGC CGACCTCCGA 240 CGCCTCATGC GCATGCTGGT CCACGCCGGC TGCTTCACCA AGCAGGAANA CNACGTGTNC 300 GCCCTCACGC CATGGTCGAN GCTCCTGGTG AGCTCCGANC ACACCTCGGT GGCCCCGTTC 360 GTGGTGTGGA TGCTCCACCC GCTCATGGTC CAGT GTGGC ACTCMCTGGG GGCGTGGTTC 420 CACGGGAAGG GCGCCCACNC CCTTCNCCNC NACCCNCGGG AAGGGGATCT TCCANACGAC 480 ACNCGAACAT CCGGGGTTCG CGGGCGTCTT CAACTANGCG ATGGCGAACG ACTGTCNGCT 540 GGTGGGAAAN GTNTTGGTAA AAAAACCTTC CGGAAGTGTT NGANGGAACC CCGTCCAATN 600 GTGGAANTTG GTGGCNGCCG GCACCCTTNC GGGCNATCGT TGTCGAAGNC CTTCCCNCAC 660 NTTANATTNC ACCGTCCTCC AATTNTCCCC ANTTTCTTTN GCCGCNGGNN NCGGGCCCCN 720 GGTNANCCNA AANCNTTTNT TTT 743 (2) INFORMATION FOR SEQ ID NO: 13: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 806 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U68 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: TGTTCTCTCC TTCGATCACA TCTTTTTGCT CTGGGAAACG TGAGAGGTGA GACGAGGGCG 60 GCAATGACGG CGGGTTTAAG ATGGATTCCT CCTCTGCTTC TTCTTCTTCT GGGCTTCCTG 120 CTGGTTTTGA ACGGAGGTCG GGGGTGGATT GGAAGCGAGA GGTCCTCTGG CTCGAGGAAT 180 GGCGGAGCAT CGCGGAGGAG CTTGAGAGAG GCCTCCGCGA ACGCGACCAG CGCCGATGCT 240 TCCTTGGAAG AGAGGGCTGT AACCCGGGCA GCAGAAGCCG CAGTCGACGA CCCCGAGGAG 300 GTTGCTTCGA CGGTCCTGAT GACCATAATC AACAGCACGG CTCGCAGATC TCTTGGTTAT 360 CTGTCGTGCG GTTCAGGCAA CCCGATCGAC GACTGCTGGC GGTGCGACCC TGATTGGCAT 420 GTCAACAGAA AAAAGCTCGC TGACTGCGGC ATTGGCTTTG GACGCAACGC NATANGTGGC 480 CGCGACGGGG AATTTGTTCG TTGTGACAGA CTCCGGGGAC NATGATCCCC GTGAATCCTC 540 GCCCGGGAAC ACTTANATAC CCCGTCNTCC AANGAAGTTG CCCCCTCTGG GATCCCCTTT 600 AAACNCNAAN TGGAAATCTC NCTCNANGGA AGAACTCNTT ATGAACAGCT TTAANACNAT 660 CGATGGACNC NGTGTCCTCG TCCACATTGC CAATGGCGCC TGCNTCACCA NCCAATTTCN 720 TCCCCAACNT CTTCTTCCNT NGCCTCCNCT CCCCAATGCA ACCCNCCGGG AATTCCTNGT 780 CCCCNCTCCC CTTCTCCTAT GGATNG 806 (2) INFORMATION FOR SEQ ID NO: 14: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 637 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown 'n, MOLECULE TYPE: cONA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U69 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: GGCACGAGGG AGAACAAAAA TGGCAGCATT CTTGTTCTTC CTCACCATCG CAGCCTTCAC 60 TGCCCCCATC TACTCTTCTC GTGCACCCTT AACGTCGGCA GCTGTCCGCG ACCCTGAATT 120 AGTAGTACAG GAAGTACAAA GAAGCTTGAA CGTGTCGCGG CGGCGACTGG GCTACTTGTC 180 ATGCGGCACC GGCAATCCGA TCGACGACTG CTGGCGGTGC GACCCTGACT GGGCTGACAA 240 CCGGCAGCGG CTCGCGGACT GCGCCATCGG GTTCGGGAAG AACGCGATTG GGGGCAGGGA 300 CGGCGAGATA TACGTGGTGA CCGACAGTGG CGACNACNAC CCCGTCAATC CGAAGCCGGG 360 CACGCTCCGG TACGCCGTCA TCCANGAGGG AGCCGCTGTG GATCATCTTC AAGCGCGACA 420 TGGTCATCCA GCTGAAGGAA GGAGCTCATC ATGAAACTCC CACANAGACC ATCGACGGCC 480 GGGGCGCCAG CGTCCACATC TCCGGGGGGC CGTGCATCAC CACCAGTNCG TCNCCAACAT 540 CATCATCCAC GGGCGTCCAC TTCCACNAAT GCAANCAGGG CGGGGAACGC CTTACGTTCG 600 CGACTCCCCC AGGGCACTAC GGGTGGCGCA CCGGTGTCCG AACGGCNANG GGGTTTTCAT 660 CTTCCGCGGG AACCACTTCT GGGGTCA 687 (2) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 741 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U84 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15: TCCACATTGC CAATGGCGCC TGCATCACCA TNCAGTACAT CACCAACGTC ATCATCCATG 60 GCCTCCACAT CCACGACTGC AAGCCCACCG GGAATGCCAT GGTCCGCAGC TCTCCTTCTC 120 ACTATGGATG GAGAACCATG GCTGATGGGG ATGCCGTTTC CATTTTCGGC TCCAGCCACA 180 TTTGGGTGGA CCACTGCTCT CTGTCCAACT GCGCCGATGG ACTTGTCGAT GCCGTCATGG 240 GCTCCACTGC CATTACGGTC TCCAACAATT ACTTCACCCA CCACAATGAG GTCATGCTTT 300 TGGGACACAC TGATTCTTAT GCAAGGGACA GCATCATGCA AGTAACGATC GCATTTAACC 360 ATTTTGGTGA AGGTCTGATT CAGAGAATGC CCAGGTGCAG GCATGGCTAC TTCCACGTGG 420 TAAACAATGA CTACACGCAC TGGGAGATGT ACGCCATTGG CGGTAGCGCG AATCCAACGA 480 TCAACAGTCA AGGCAACCGA TACCTTGCGC CGACCAATCC ATTTGCAAAG GAAGTAACAA 540 AAAGGGTGGA CACAGATCAA AGCACGTGGA AAAACTGGAA TTGGANGTCN GAAGGTGACC 600 TGCTTCTGAA TGGTGCTTTT TTCACCCTTC CGGTGCANGG GCTTTCACCC ANCTACGCAC 660 GGGCCTCCAC TTTGGGGGCA ACCCTCTTCC TTNGTTGANA CACTGACTCT GATGCTGGGG 720 TCCTGTTTTG CCAATTCGGN C 741 (2) INFORMATION FOR SEQ ID NO: 16: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 727 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: Single (D) TOPOLOGY: unknown (ll) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U89 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16: GGCACGAGTT CTCTCCTTCG ATCACATCTT TTTGCTCTGG GAAACGTGAG AGGTGAGAAG 60 AGGGCGGCAA TGACGGCGGG TTTAAGATGG ATTCCTCCTC TGCTTCTTCT TCTTCTGGGC 120 TTCCTGCTGG TTTTGAACGG AAGTCGGGGG TGGATTGGAA GCGAGCGGTC CTCTGGCTCG 180 AGGAATGGCG GAGCATCGCG GAGGAGCTTG AGAGAGGCCT CCGCGAACGC GACCAGCGCC 240 GATGCTTCCT TGGAAGAGAG GGCTGTAACC AGGGCAGCAG AAGCCGCANT CGACGACCCC 300 GAGGAGGTTG CTTCGACGGT CCTGACGACC ATAATCAACA GCACGGCTCG CAGATCTCTT 360 GGTTATCTGT CGTGCGGTTC AGGCAACCCG ATCGACGACT GCTGGCGGTG CGACCCCGAT 420 TGGCATGTCA ACAGAAAAAA GCTCGCTGAC TGCGGCATTG GCTTTGGCCC GCAACGCGAT 480 ANGTGGCCGC CACNGGGANT TGTNCNTTGT TGACAGACTC CGGGGACGAT GATCCCGTGA 540 ATCCTCCCCC GGAACACTTA ATATTCCCCG TCATCCCAGA NCTNCCCCTC TGGATCNCCT 600 TTTAAACACG ACATTGGAAA TCNCCCCCNG GAAGAACTCC TTTNTAACCG CTTTNNAACA 660 TCNATGGACN CGTGTCCACN TTCCACNTTT GCCAATGGCG CCTGCCTCNC CANCCNATTT 720 AATCNCC 727 (2) INFORMATION FOR SEQ ID NO: 17: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 842 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (11) MOLECULE TYPE: cDNA (v i) IMMEDIATE SOURCE: (B) CLONE: U-U115 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: GGCACGAGGG AGAACAAAAA TGGCAGCATT CATGTTCTTC CTCACCATCG CAGCCTTCAC 60 TGCCCCCGTC TACTCTTCTC GTGCACCCTT AACGTCAGCA GCTGTCCGCG ACCCTGAATT 120 AGTAGTACAG GAAGTACAAA GAAGCTTGAA CGTGTCGCGG CGGCGACTGG GCTACTTGTC 180 ATGCGGCACC GGCAATCCGA TCGACGACTG CTGGCGGTGC GACCCTGACT GGGCTGACAA 240 CCGGCAGCGG CTCGCTGACT GCGCCATCGG GTTCGGGAAG AACGCGATTT GGGGCAGGGA 300 CGGCGAGATA TACGTGGTGA CCGACAGTGG CGACNACGAC CCCGTCAATC CGAAAACGGG 360 CACGCTCCGG TACGCCGTCA TCCAGGAGGA GCCGCTGTGG ATCATCTTCA AGCGCGACAT 420 GGTCATCCAG CTGAAGGAGG AGCTCATCAT GAACTCCCAC AAGACCATCG ACGGCCGGGG 480 CGCCAGCGTC CACATCTCCG GCGGGCCGTG CATCACCACC AGTACGTCAC CAACATCATC 540 ATCCACGGCG TCCACATCCA CGAATGCAAG CAGGGCGGGA ACGCGTNCGT TCGCNACTCC 600 CCAAGGGCAC TACGGGTTGG CGCACNGTGT TCNGACGGCG ACGGGTGTCC ATCTTCCGCG 660 GGAACCACTC TGGGTCCAAC CACTGCANCG CTTGTTCCAA CTTGGCCCAC CNAANTGGGC 720 CTTCCNTCCC AACCCCCATT TCCTTGGGAT TNCCCCCCGC CAATTCCCCC ATTTTCCAAC 780 NAACTTNCTT TGAACCCNCC CNTGAANAAG GTCCTTGCTG CTGGGTCCCA CCAACAATTT 840 NA 842 (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 724 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U117 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18: GGCACGAGTC TCCTTCGATC ACATCTTTTT GCTCTGGGAA ACGTGAGAGG TGAGACGAGG 60 GCGGCAATGA CGGCGGGTTT AAGATGGATT CCTCCTCTGC TTCTTCTTCT TATGGGCTTC 120 CTGCTGGTTT TGAACGGAGG TCGGGGGTGG ATTGGAAGCG AGAGGTCCTC TGGCTCGAGG 180 AATGGCGGAG CATCGCGGAG GAGCTTGAGA NAGGCCTCCG CGAACGCGAC CAGCGCCGAT 240 GCTTCCTTGG AAGACAGGGC TGTAACCAGG GCAGCAGAAG CCGCAGTCGA TGACCCCGAG 300 GAGGTTGCTT CGACGGTCCT GACGACCATA ATCAACAGCA CGGCTCGAAN ATCTCTTGGT 360 TATCTGTCGT GCGGTTCAGG CAACCCGATC GACNACTGCT GGCGGTGCGA CCCCGATTGG 420 CATNTCAACA GAAAAAAGCT CGCTGACTGT GGCATTGGCT TTGGCCGCAA CCCGATANGT 480 GGCCGCGACG GGGANTTGTN CGTTGTGACA GACTCCGGGG ACGATGATCC CCNTGAATCC 540 TCCCCCGGGA ACACTTANAT NCCCGTCNTC CNNGAANTTT CCCCTCTGGA TCNCCTTTAA 600 ACCCACNTGG ANATCCCCCN CNAGGAAGAA CTCCTTATGA ACNGCTTTTT AAAACATCGA 660 TNGGACCCNG TTTCCACTTC CNCATTNGCC MAATTGGGCG CCCTGGCCNT CCNCCCNTCC 72C CCAA 724 (2) INFORMATION FOR SEQ ID NO: 19: (1) SEQUENCE CHARACTERISTICS: (A) LENGTH: 797 base pairs (3) TYPE: nucleic acid !C) STRANDEDNESS: single (D) TOPOLOGY: unknown (11) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U80 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: GGCACGAGGG ACAAGCGATG GCAGCAGAAT TGTCTCCCAC GCTGAGCAAG AGCATCTTTG 60 AAGGAGCCGG TGGATCTTAC TCTACTTGGT CAGGTGCCGA TCTCCCCCTT CTTACTGATG 120 CAAAGCTCGG CGGAGGCAAG CTTGTCCTGA AACCACTGGG CTTGGCGTTG CCTCACTATT 180 CCGACTCATC GAAAGTCGGC TATGTTCTTG AAGGAAGAGC GGTGGTGGGG CTAACACTCT 240 ATGGAGAGAC CGAGCAGAGG ATACTGCTGC TTGAGAAGGG AGATGTGGTA GCGGTGGTCA 300 TGGGGAGCCT CACGTGGTGG TACAACGAGG AGGAGGACTC CGACTTCTCC ATCGCCTTCT 360 TAGGCGATAC CGCGACAGCT GTGCGACCGG GCGACATCGC CTACTTCTTC TTGGCAGGAT 420 CCCTAGGAAG TGCTCCATGG CTTTTCGACG GAAATTCCTC ANCAGGGCCT GCGGTTTAAG 480 GGGATGCGGA AGCTGANGAG CTCTTCGGAA GCCAACCTGG TACTCTAATC ATCACACNGC 540 AGCAAAAGCT GCCTGGCCTC AGANCATCCC GAGCTGACAG CNAAGGGATA TCNTAAACGC 600 TGANCGCGTA NCGGCATATA TCAATGTGAA AAGTGGTGGC TGTTCTGCCT CGGTGACCCT 660 TNATNAACTG GCNGCNCTGG GAAGATCAGG TTCTCCGTCN ACCTCCAAAA ATCCANCCTA 720 ACCCCGTGCC TTGCCGGGGT TCTTCNTTGA ATCNNCTGTT CANCTGAAAT TTTTCCCCAA 780 GGCCNTGGAA CAATTCT 797 (2) INFORMATION FOR SEQ ID NO: 20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 742 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U90 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20: GGCACGAGAT GACTCTCTCG GCGGTAGCAT CGGATGCCGA TGATTCGGTC GCTTATACAT 60 TCGCTTCGCG ATACGTTCGC GAGGCTCTTC CCCGGTTCAG GATACCGGAG CAGTCGATCC 120 CCAAGGATGC GGCGTACCAG ATCATCAACG ACGAGCTGAT GCTCGACGGG AACCCGCGGT 180 TGAATCTGGC GTCGTTCGTG ACGACGTGGA TGGAGCCGGA GTGCGATCGC CTCATCATGG 240 CGGCCGTCAA CAAGAACTAC GTCGACATGG ACGAGTACCC CGTCACCACC GAGCTCCAGA 300 ATCGCTGCGT AAATATGATA GCCCACCTTT TCAATGCCCC AATTGGGGAA NACGAAACGG 360 CTGTTGGAGT TGGAACTGTG GGTTCCTCCA GAAGCAATCA TGCTTGCAGG ACTTGCATTC 420 AAGAGGAAAT GGCAGAACAA AAGAAAGGCA GAGGANAAGC CTTACGACAA ACCCAACNTT 480 GTTACCGGTG CAAATGTTCA GGTTTGCTGG GANAAATTTG CAAGGTATTT TGAAGTTTGA 540 ACTGAAAGAA ATTGAAGTTG AAAGAAGGAT ATTATGTTAA TGGATCCTGC CAAGGCAGTA 600 NAAATGGTTG ATGAAAAATA CATATGTTTT TGCTGCCATC TTTGGGTTCA ACTCTCCCTG 660 GGAAAATTTG AANATGTTAA CCTTCTAAAT GATCTCCTGA CNGAAAAAAA CCCAGAAACT 720 GGGTTGGACC CCCCNTACTT GT 742 (2) INFORMATION FOR SEQ ID NO: 21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 807 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U92 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21: GGCACGAGCT CTAGCCGATG ACTCTCTCGG CGGTAGCATC TGATGCCGAT GATTCGGTCG 60 CTTATACATT CGCTTCGCGA TACGTTCGCG AGGCTCTTCC CCGGTTCAGG ATACCGGAGC 120 AGTCGATCCC CAAGGATGCG GCGTACCANA TCATCAACGA CGAGCTGATG CTCGACGGGA 180 ACCCGCGGTT GAATCTGGCG TCGTTCGTGA CGACGTGGAT GGAGCCGGAG TGCGATCGCC 240 TCATCATGGC GGCCGTCAAC AAGAACTACG TCGACATGGA CGAGTACCCC GTCACCACCG 300 AGCTCCAGAA TCGCTGCGTA AATATGATAG CCCACCTTTT CAATGCCCCG ATTGGGGAAN 360 ACNAAACGGC TGTTGGAGTT GGAACTGTGG GGTCCTCAAA AGCAATCATG CTTGCAGGGC 420 TTGCATTCAA GAGGAAATGG CANAACAAAA GAAAGGCAGA GGANAAGCCT TACGACAAAC 480 CCAACATTGT TACCGGTGCA AATGTTCNGG TTTTGCTGGG AANAAATTTG CAAGGTATTT 540 TGAAATTTGA ACTGANAGAA NTGAANTTGA AAGAAGGATA TTATGTTTAT GGATCCCNGC 600 CCAAGGCAGT ANAAATGGTT GATGAAAATA CCAATTTNTG TTGCTTGCCC ATCTTTGGGT 660 TCCACTCTCN CTGGGANAAT TTTGAAAAAT GTTNAGCTTC TAAATGATCT CCNGGACCNA 720 AAAAANNCCC NNAANCTNGG TTGGGACCCC CCCNTACCTT TTCNATNCCT GCCANTTNGG 780 GGATTCNNTA NCGCCTTTCC TNCTATC 807 (2) INFORMATION FOR SEQ ID NO: 22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 770 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown ( ) MOLECULE TYPE: cDNA (vi ) IMMEDIATE SOURCE: (B) CLONE : U-U91 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: GGCACGAGAG AAAAAATTGT AAAGTTTGAC TTTCATGGAC AGCCTGCGGA GCTCAAGCAT 60 GGTAGCCTTG TCATAGCAGC AATTACAAGC TGCACAAACA CATCAAATCC CAATGTTATG 120 CTTGGTGCTG GTCTTGTTGC AAAGAAGGCC TGTGAATTAG GTCTACAGGT TAAGCCTTGG 180 ATCAAAACCA GCCTTGCTCC AGGTTCTGGA GTTGTTACCA AGTATTTGCT TAAGAGTGGC 240 CTACAAGAAT ATTTGAATCA GCAAGGATTT AATATTGTTG GGTATGGCTG CACAACATGT 300 ATCGGAAACT CTGGGGATCT TGATGAGTCT GTGGCTGCTG CAATTTCCGA TAATGATATA 360 GTTGCTGCTG CTGTTCTATC TGGAAATAGG AATTTTGAGG GACGTGTGCA TCCATTGACA 420 CGAGCTAACT ATCTTGCTTC ACCTCCGCTT GTTGTAGCTT ATGCGCTTGC TGGAACAGTT 480 GACATTGATT TTGAAAAAGA ACCCAATTGG AACAGGGGAA GGATGGGAAA AAACATATAC 540 TTCCAGGATA TATGGCCGTC CACTGAANAA ATTGCACAGG TTGTGCAATC CANTGTTTTT 600 GCCTGAAATG TTCAAGAATA CCTATGAAGC AATCACCAAA GCACCCTATG TTGGAACCAA 660 CTGACTGTTC CCNGCCACAC TCTTTATTCA TGGGACCCAA TCCNCNTACA TCTTAACCAC 720 CNTATTTTAA GGANATGACC ATGGCCCNCC TGTCCNCATG GGGTGAAAAA 770 (2) INFORMATION FOR SEQ ID NO: 23: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 810 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U96 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23: GGCACGAGAG GGCCGTTCCT TTTTGTGCTC GCTTTGCTGC CTCCTCGTTC GTCTTTTCCG 60 CGAATTTCAC CGGCCATGAA CAGCCTTCGG AGCCTTCCGA CATCCTCCGC TCCGCCCTCG 120 CCGGTCCCGA GAGGACGTTC GAGTAGGCCA CCGACCTTGA ATCTGGGGGT GATACGACCA 180 CATCATCTTC GTGTAAATGT CGCCAGGAGT GCATGTTCCA AGTCTTCTGC AAGTCCTGAG 240 AAGACTGGTG CTGAAGTGCA AGATCAGAAG TCTGAGATAT ATAGCAGTGA TATGACAAAA 300 GCAATGGGTG CAGTGCTGAC ATATAGGCAT GAGCTTGGAA TGAATTATAA TTTCATCTAC 360 CCAGGATTGA TTGTTGGTTC TTGTCTACAG ACCCCATCAG ATGTTGATAA GCTTCCNAAA 420 GATTGGGGTT GAAGACCATC TTTTGCTTGC AGAAGGACTC GGATCTTTGA ATATTTTGGG 480 GTTGAAATTG GTGCAATTCC NTGAATATGC CATGCAATGT GGTGACATTG AACATCTTCN 540 TGCTGAAATC AGGGATTTTG ATGCATTTGA TTTGAAGATG ANACTTCCTG CNGTANTTAC 600 NAATTATACA AGGCTAATTA ATCCGAATGG TGGAATGACT TATTTTCNCT GTACANCCNG 660 GCTTGGAAAA CGCCTGCANT TGCNTTGGCA TACNTGTTTT TGGGTTNAAG GCTATGAACT 720 GAATGAAAGG GCCCCNCTAC TGCNAAATTA NCCAACTTGC TCCCCNAAAT TTGGTGCTAT 780 AAAAANTGCC ACCNTTGATA TGCTTATNGG 810 (2) INFORMATION FOR SEQ ID NO: 24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 765 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U103 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24: GGCACGAGCT TGGCTGTAGT CTTCTTCTCA AGTAATCTTC GTCTTCCTGA TTTGCTTGGT 60 TGGTGGTCGG TTGCAGGGCG AAGGGATGGG GATGGGGAGG CCGAGCGGAG CTTGGTCGAC 120 CCCGTGGTTG GTGGTGGTTC TTGTGCATTG GCTCCTCTGG GCCACCGAGA GGAGGCGAGG 180 GGCGGTGGTG GAGGCCTCCC ATGTGGAGTT TGCATCCCTC CAATCTGTTC CTGCCTCCGT 240 CGTCGACAAC AGGCTGAGGA CTGGGTATCA CTTCCAGCCC CCGAGGAACT GGATCAACGA 300 TCCAAATGGA CCCATGTACT TCAATGGCGT CTACCACCTC TTCTACCAGT ACAACCCCAA 360 TGGCTCCGTG TGGGGTAACA TCGTGTGGGC CCACTCGGTG TCGACCGACC TCGTCAACTG 420 GATAGCACTC GACCCGGCCA TCCGCCCCAG CAAGCCATTC CACATCAACG GATGCTGGTC 480 CGGCTCCGCC ACCGTCCTCC CCGGCAACAG GCCTGCGATC TTCTACACCG GCATCNACCC 540 CCANCANAAA CAACTGCANA ACNTTNCNTN CCCAAGGATC TGTCCAACCT TACTCCCCNA 600 NTGGGTCAAC CCCACTNCAA CCCCGTGATC CCCCTGGCGA CGCATCAACN CCACCCCTTC 660 CNCNAACCCN AATACGGNTT GGCGCNGTCC CNGCACCCNC TGGAAACCCC TNGTNGGGAA 720 CNANTTGAAN CNGAAGGGGA AAGGCNTTCT TTNCCGAACA GGGAT 765 (2) INFORMATION FOR SEQ ID NO: 25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 774 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U93 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25: GGCACGAGCG GAGAANGCAA TCTCGCTCTC TCTCTACTGC ATCGCGGCTC TCGTCCTCGA 60 TTACTTGAGA TATGGCAGGA AAGGGCGAGG GGCCGGCGAT CGGGATCGAT CTCGGCACCA 120 CGTACTCGTG CGTCGGGGTG TGGCAGCACG ACCGGGTGGA GATCATCGCC AACGATCAGG 180 GGAATCGTAC CACCCCCTCC TATGTCGCCT TCACCGACTC CGAGCGCCTT ATCGGCGACG 240 CTGCCAAGAA CCAGGTCGCC ATGAACCCCA TCAACACCGT CTTTGATGCA AAGCGTTTAA 300 TTGGTAGGCG ATTCAGTGAT TCATCTGTCC AGAGTGACAT CAAGCTCTGG CCTTTCAAGG 360 TCATTCCTGG TCCAGGTGAC AAGCCCATGA TTGTTGTCCA ATACAAAGGG GAGGATAAAC 420 AGTTTGCAGC TGAAGATATT TCTTCCATGG TTTTGATAAA GATGAAAGAA ATTGCTGAAC 480 CTACCTGGGG TCTGTTGTCA AGAATGCCGT CCTCACTGTT CCCGCTTACT TCAATGACTC 540 NCAACGCCCA GCCACAAAGG ATGCTGGTGT CATTGCTGGT CTCNATGTTA ATGANAATCA 600 TCAATGANCC CCCAGCACTG CTATTGCTTT ATGGCCTTGA CAAAANGCTA CTATTGTTGG 660 TGAAAAAAAT TTCCTCATCT TCCATCTCCG GTGGGTGGCC TTTTGANTTC NCCTTCCTNA 720 CCATCCAAGA AGGTTTCTTT NAAGTCAAGG CCCTGCTGGT GANACCCANC TTGG 774 (2) INFORMATION FOR SEQ ID NO: 26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 786 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U125 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26: GGCACGAGCT AGTCTCGAGT TTTTTTTTTT TTTTTTTTAA AATTGAAATT AGATGCGATT 60 TCGAATAGCA AACATAGTTC AGGATAAACC AACAACATTG TACCGAATTC GATAAGCTTA 120 GGAAACTAAA TTGCGTAAAC GAAACACTTG CATGAAAGCC TATATAATAT AAGGCCGACT 180 AGGGACNAAA TAACTTAATC GACCTCCTCG ATCTTTGGAC CAGCGCCGCT GCCACCAGCA 240 GGAGGAGCAT CATCATCCAT ACCACCAGCC ATGTCAGCAC CTGCTCCCTG GTACATCTTG 300 GCGATGATGG GATTGCATAT GCTCTCCAGC TCCTTCATCT TGTCATCGAA CTCGTCGGCT 360 TCTGCCAACT GGTTGCCATC GAGCCATTGG ATGGCCTGCT CGATCGCATC CTCCGATCTT 420 CTTCTTGTCA GCAGCAGCAA GCTTGGAGGC AATCTTGTCG TCGTTAATGG TGTTCCTCAT 480 GTTGTNAGCA TANTTTTCCA GANCATTCTT CCGATTCCAC CTTCCTTTTT ATGCTCCTCC 540 ATCTTCCTGA CTTGTTACTT CTCCGCTTCC CTGCACCATT TTCTCAATCT CCTCCTTGCT 600 GACCTACCTT GTCCTTGGTG AAGGTGATCT TGTTCTCTGT CCAGTGGTCT NTCNTCNGCA 660 GAAANATTCC AGAATACCAT TGGGGTTCCA AATGTTNCAA AACCAAACCC GGTGGATTCT 720 TGAAGGAAAC CCCCCCCTAA GGGCCCCAGG GTGGGAAATC CCCCCAAAAA ACCCCCAAAT 780 TTTCCC 796 (2) INFORMATION FOR SEQ ID NO: 27: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 486 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown ( i) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U105 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27: GGCACGAGCG AGTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTCACAAG GNAACCATAG 60 ACATTACACC AAACATAGAT GTCAACAATA CATGCCAACG ATACCATTGC TTATTCCTTC 120 AGACCTCCGA TAGGACTTCC ACGCACACCA ACAGAGAGAA CTAAGACAGC AAAGCAGCGG 180 ACACATACAG CCCAAAAGGG TNNGTGACCC CCGCGGAGGN GGAGCNCCAG GTGAAGGGTG 240 GATTCCCTCT GAATGTTGTA TTCTGNCAGG GTGCGCCCGT CCTCNNTTTG NTTCCCNTCT 300 NANATCNCCC NCTGNTGATC TGGTGGNAAT CCCCCCCTTG TCCTGTGATT CTTGCTCTTG 360 ACNTTTTTCT CCCNTGTCNN ANCTCTCTNC CTNCTCTGGG TNTTGGGTCT TCCCCTGTTG 420 NANCNNTCTN TTNNCNTATC ATCTTGTNCT CCNNCCNCCN CNCCTNTCNT TTCCTCNNNN 480 GGTNNA 486 (2) INFORMATION FOR SEQ ID NO: 28: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 791 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (n) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U116 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28: GGCACGAGTG ACAATGTCAA GGCCAAGATT CAGGATAAGG AGGGCATTCC CCCCGACCAG 60 CAGAGGCTCA TCTTTGCCGG CAAGCAGCTC GAGGATGGCC GCACCTTGGC TGATTACAAC 120 ATCCAGAAGG AGTCCACCCT CCACCTTGTC CTCCGCCTCC GTGGTGGCAT GCAAATCTTC 180 GTCAAGACTT TGACTGGGAA GACCATCACC CTTGAGGTGG ANAGCTCGGA CACCATCGAC 240 AATGTAAAGG CCAAGATTCA GGACAAGGAG GGTNTTCCCC CGGACCAGCA AAGGCTCATC 300 TTTGCCGGCA AGCAGCTTGA GGATGGCCGC ACCCTGGCAG ATTACAACAT TCAGAAGGAG 360 TCTACCCTTC ACCTTGTGCT GANACTTAGG GGTGGCATGC ANATCTTTGT TAAGACNCTC 420 NCAGGGGAAG ACCATTACCT TGGAGGTGGA NANCTCNGAC ACGATTGATA ATGTCNAGGC 480 AAAGATCCAN GACAAGGANG GGATTCCACC GGATCAGCAN ANGCTGATCT TTGCTGGGAA 540 ACACTGGANG AAGGGCGCAC CCTGGCAGAT TNCNACATTC CNAAAGAATC CACCTTCACC 600 TGGTGCTCCC CCTCCGCCGG GGTCCCTAAC CCTTTTGGGC TGTATGTNTC CCCTGCCTTT 660 GCTGTCTTAA TTCNCTCTGT TGGTGTTCGT TGGAAATTCC CTAATCCNGA AGGTTCTTNA 720 AAAGGAAATN AANCCNNTTG GGTTTTCCCC TTTGGCCTTT TTTTTGTTTG AAAATCCTNT 780 GTTTTTGGTG T 791 (2) INFORMATION FOR SEQ ID NO: 29: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 814 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U120 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29: GGCACNANCC CGCTTCGGTA GCCATTATTG GTGCCCGTAG AGCGAGTGCT GAGATGGCTT 60 TGAGGGCCTT CTTCCCCCTC TGCATCGCTC TCGTGGTGAC GCTCTCACCT CTGTGTGATG 120 CCACTTCGCC TTACTACACC ATCACACCGC CCACCCCCGT GGCCAAGCCG CCTTCAGTTG 180 AACCACCACC CTACCACGGC CCTCCGACNA CCCACCCTAA GCCACCGAGT CATGGTGGCC 240 AACCTCCGTC CCACCATCAC CCAACACCAA TCTACGGTGC ACCCCCTCCG CAACACCACC 300 ACCACCACCA ACACCACCAC CAACCTGCAC CACCAACTCA CGCANAACAC CCTCCGTACT 360 ACCACATGCC TTCCCCGCCG CCGCATGGCC AGCACCCGTC ACCACCGTCA CATGATTATC 420 CCGTACCTCC TGCTCACAAG CCCCGAACTC CGCCGCCGGT TTACAAGTCT CCACCACCGA Θ0 NCCACCGTCC TTACCCTCCA TCGACGCCAC CCCACCATCC GAAGCACCCG CCTTCTCAGC 540 CGAAGCCGTC NTACAAGGCC CGCCACCATN CAAGAACATC CCTGANCACT CTCCACCGCC 600 GCGTCACTAT CATTCTCCGT CTTCACCACC ACCACCACCA CCATACAAAA TAATCTCGTT 660 TTGCCATCTT CTCCCGTTTG AAAAAAAANN CAANTGTCCT TTTTATTTAA CCNAATCCAN 720 GGGGTTTTTN TTAANATTTN AANAAANCNA ANTNTTTTCC CCCCCNTNTT GGTTNATGGC 780 CTATGGGTTT GTTTCTCTTC TTGGCTTTTC CCGG 814 (2) INFORMATION FOR SEQ ID NO: 30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 742 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown Ui) MOLECULE TYPE: cDNA (vu) IMMEDIATE SOURCE: (B) CLONE: U-U126 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30: GGCACGAGGT TGTGCCTAAC AGAGAGAGAG AGAGACAGAC CGATAGCCTC CTCATTCACT 60 ATGGCGATCC GATCGCCAGC TTCGCTGCTG TTATTTGCGT TTCTGATGCT TGCGCTCACA 120 GGAAGACTGC AGGCCGGGCG CAGCTCATGC ATTGGCGTCT ACTGGGGACA AAACACAGAC 180 GAGGGAAGCT TAGCAGATGC TTGTGCCACA GGCAACTACG AATACGTGAA CATCGCCACC 240 CTTTTCAAGT TTGGCATGGG CCAAACTCCA NANATCAACC TCGCCGGCCA CTGTGACCCT 300 CGGAACAACG GCTGCGCGCG CTTAAGCAGC GAAATCCAGT CCTGCCAGGA GCGTGGAGTC 360 AAGGTGATGC TCTCCATCGG AGGTGGCGGG TCTTATGGCC TGAGTTCCAC CGAANACGCC 420 AAGGACGTGG CGTCATACCT CTGGCACAGT TTCTTGGGTG GTTCTGCTGC TCGCTACTCT 480 CNACCCCTCG GGGATGCGGT TCTGGATGGC ATANACTTCA ACATCCCCGG AGGGAGCACA 540 GAACACTATG ATGAACTTGC CGCTTTCCTC CNAGGGCTAC AACGAACAGG AAGCCGGAAC 600 CAAAAAAGTT TCACTTGAAT TGCTGCTCCC NCANTGTCCT TTCCCCGATT ACTGGCTTTG 660 GCAACCCACT CCAAAACAAA TNTCTTCCNA CTTCCNTGTT GGGTTGCNAA NTTCCNTTCC 720 CAANCAAACC CCTTTCNTTT GC 742 (2) INFORMATION FOR SEQ ID NO: 31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 794 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U129 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31: GGCACGAGGG CGTGGCGGAG ATGGGGAGCT GGCGGGCTCT GTTGCAGCGG CGGCTGCTGT 60 TGCTCTCTGC TTTGGCGGTG GCTGTTCGTG TGAAGGCACT CAGCANANAC NATTTCCCCG 120 CCGGCTTCAT TTTTGGCGCA GGCACCTCCG CTTATCAGGT ANAAGGTGCA GCTGCAGAGG 180 GGGGAANAAC ACCCAGCATT TGGGACACGT TTACGCATGC AGGGAGAACT TTCGACCAGA 240 GCACCGGAGA CGTAGCAGCT GATCAGTATC ACAAGTACAA GGAAGATGTG AANCTGATGC 300 ATGANATGGG CTTCGATGCT TACAGATTCT CCATCTCCTG GTCCANAGTT ATCCCCAATG 360 GTCGAGGGCC TGTGAATCCA CAAGGCTTGC GGTACTACAA CAACCTGATC GATGAGCTCN 420 AAAGATATGG AATCCGAGCC TCATGTCACT CTTTACCACT TCGACCTTCC GCAAGCACTG 480 GAANACGAAT NCGCCGGGCA GCTGAACCCA AAGATCGTAN ANGACTTCAC CGCTTACGCC 540 AACGTTTTGC TTCANCNAAT TTGGGGATCN AATCTAGCAC TGGATCNCCC ATCAATGAAC 600 CAACATANAT CCCGTCCTCC GCCACNAATT CCGCATCTTC NCCCCTGGCC CCTGCTCTTT 660 ATCCCTTNCG CCTCCAACTG CNCCAAGGGC NACTCCCCCC CATTTTNAAN CCCATNNNNT 720 CCNCCCCCCC CAATTAACCC NTTCTTGCTC TTCCCCCTTG CCTTCANCCA ACCCGCCCNT 780 TTTCCNAAAA AANA 794 (2) INFORMATION FOR SEQ ID NO: 32: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 799 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U130 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32: GGCACGAGCC GGCCTCAGGC ATATGGTGCG GTCGCTACAC GTGTTCAGGC AAGGCCGGGA 60 GCCGGCCAGG ATCATCAGGG AAGCACTGTC GAAGGCGCTG GTGAAGTACT ACCCCTTCGC 120 GGGGCGGTTC GTGGACNATC CCGAGGGCGG CGGCGAGGTT CGTGTCGCTT GCACTGGCGA 180 GGGCGCTTGG TTCGTCNAGG CCAAGGCGGA CTGCAGCTTG GAGGACGTGA AGTACCTCGA 240 TCTCCCGCTC ATGATCCCTG AGGACGCGCT CCTGCCCAAG CCCTGCCCGG GACTGAACCC 300 CCTCGACCTC CCTCTCATGC TGCAGGTGAC ANANTTCGTG GGCGGCGGAT TCGTGGTCGG 360 CCTCATCTCC GTCCATACCA TCGCCGACGG CCTCGGCGTC NTCCANTTCA TCAACGCCGT 420 CGCCCGAAAT CGCCCGTGGC CTGCCNAANC CCACCGTGGA NCCTGCATGG TCCCGGGANG 480 TCNTACCCAA CCCACCTAAG CTGCCTCCCG GTGGCCCGCC CGTGTTTCCC CTCCTTCAAN 540 CTGCTCCACG CCACCGTCGA ACTATCCCCT GACCACATCC ATCACGTCAA GTCCCCACAC 600 TTTGGANCTC ACCCGGCCAA CGCTGCTCTA CCTTCCACNT CCCCATCCCC AACCTGTTTG 660 NAATCCCGCA CGCCCCGCCN TCNACCTTGG AACCCAAGGC NTTTCCAANC TTTGCAACCC 720 TTTTTTCCTT TCCTTCCCCC CCCAAAANAA CTTCCCCCCA CCCTTGTTTT TGCNCCCAAG 780 TTTTTTCCTT CCTTGCCCC 799 (2) INFORMATION FOR SEQ ID NO: 33: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 759 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown Ul) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (3) CLONE: U-U43 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33: GGAGGTTGTT GTTCATGTCC CTACTTCGGG GTCTCCTGTA CAGATTGAAT TTCAAGTAAC 60 TAATAGCAGT GGCTACTTGG TGCTTCATTG GGGTGCAATT CATAATAGAA GGAATAACTG 120 GTCACTTCCT TCTCGTCATC CTGATGGAAC AAAAGTATAC AAAAACCGAG CTCTCAGGAC 180 ACCTTTTAAA AAATCTGGTT CCACATCATC AGTAAAAATG GAGATTGATG ATCCTGAAAT 240 ACAAGCAGTT GAGTTTCTCA TATTTGACGA GTCAGAAAAC AAATGGTTTA AACATAATGG 300 TCAGAATTTC CATGTCCAGT TATTAAAACA GGGCTATCAA AATCAAAATG TTTTGGCCTC 360 TGTAAATCCA AATGTTTCAC TGCCANAAAG AACTTGTGCA GATTCAAGCT TATCTTAGGT 420 GGGANANAAA GGGTAGGCAA ACATATACAC CTGATCAAGA AAAGGATGAG TNTGAAGCAG 480 CTANAACTGA GTTTGCTANA AAAACTAAGT NNAAGTACTT CTGTANANGA NCTCCGATCT 540 AAATTGACAA AAAACNCCAC TGCTGGAGCG GACAAATCCG TCAAAGTTGA AACTATCAAA 600 AGTGGGATCC GGATGATCTT NTGCAAGTTA CANGCATACT TCCNATNGGA AAAACCGGGA 660 AACCATNTTA TCCCCCNGAA AAACACTNAT GGAATTGAAG AAACCAGGGA AANACTGCCC 720 ATGAATTAAA TAAANGAACN CNCCTNCAAA CTNAGGGAA 759 (2) INFORMATION FOR SEQ ID NO: 34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 785 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U70 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34: GGCACGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG AGAGAGAGAG 60 AGAGTAATCG ATTTAGCGTG CAAAGTACTG CAGCAGAAGC CATGGCATCC ANGGCCTCAG 12C CNTCGGCTGC CCTCTTCCTT GCTCTCAACC TTGTCTTCTT TGCACTCACC AGTGCTTGTG 180 GCACCACTTG CACCCCCGCC ACTAAGCCCC CACCGAGCTC TTCCCATGGC AAATGCCCCG 240 TCGACACCCT CAAGCTGGCC TCCTGCGCCA ACGTGCTCAA TGGCCTGATC NNGGTCGGCG 300 TCGGCAAGCT CCCGAAACAG CCGTGCGAGT GCTGCNCTCT GCTCGATGGC CTCGTCGACC 360 TCGAGGCCGC CGTGTGCCTT TGCNCTGCTA TCANGGCCNA TGTCCTTGGC ATTCATCTCA 420 ACCTCCCCAT CNNCTTCAGC CNTCTCCTCA ACTACTGCGG TNAGAAGGCT CCCACCGGTT 480 TCCAGTGCCC TTGAGCTCCC CCTGCGGATC ACATCNTCGA TCCTCTTTGC CCGTGGTGAA 540 TTTTMTTGAG CATTCCTTTG TGTCTTTACT TCCCTTTGTT TTTGGCTTTT TGATGACGAA 600 AGTGTTGAGT GTTGATATGA AGGGCCANTG GCGCTTTCNT GTTAATGTTA CCTGTCNTTC 660 CCTTTTTATT TCCACNNCTT GTTGANTTTC CCTCTCGGGT TTGTTAATGA AGATCNATGC 72C ATTCAATTNT TAATATTTCT GCTGCCTTCC AANAAAAAAT ANNANTMCCT CCNNGAANTC 78C TTCCT 785 (2) INFORMATION FOR SEQ ID MO: 35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 784 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U16 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 35: CGGCACGAGG AAANGACNAG CGATGGCAGC AGAATTGTCT CCCACGCTGA GCAAGAGCAT 60 CTTTGAAGGA GCCGGTGGAT CTTACTCTAC TTGGTCAGGT GCCGATCTCC CCCTTCTTAC 120 TGATGCAAAG CTCGGCGGAG GCAAGCTTGT CCTGAAACCA CTGGGCTTGG CGTTGCCTCA 180 CTATTCCGAC TCATCGAAAG TCGGCTATGT TCTTGAAGGA AGAGCGGTGG TGGGGCTAAC 240 ACTCTATGGA GAGACCGAGC AGAGGATACT GCTGCTTGAG AAGGGAGATG TGGTAGCGGT 300 GGTCATGGGG AGCCTCACGT GGTGGTACAA CGAGGAGGAG GACTCCGACT TCTCCATCGC 360 CTTCTTAGGC GATACCGCGA CAGCTGTGCG ACCGGGCGAC ATCGCCTACT TCTTCTTGGC 420 AGGATCCCTA GGAGTGCTCC ATGGCTTTTC GACGGAATTC CTCANCAGGG CCTGCGGTAT 480 AAGGGATGCG GAANCTGAAG AGCTCTTCGG AAGCCAACCT GGTACTCTAA TCATCNCACT 540 GCANCAAAAG CTGCCTGGCC TCANAAGCAT CCCGAGCTTG ACNGCGAAGG GATAGTCNTN 600 AACNCTNANC GCGTTNCGGC ATATATCAAT GTNAANAGTG GTGGCTGTGC TGCGTCCGTG 660 ACACTTNATG AACTGGCCGC GCTGGGAAGA TCTGTTCTCC GTCNAACTCA CCANANTCNA 720 ACCTAACCCC NTNCNCTTCC NGGGTTCTCC TTNATGCACT NTGCNCCTAT TTNTTTCCCA 780 ANGC 784 (2) INFORMATION FOR SEQ ID NO: 36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 817 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U30 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36: CGGCACGAGA TATGGAGGTA TGATCGGGAG CTACTGGGAG CTCCGCTTTG GTGAACAACA 60 CGACGGCAAG AAGGGCTATG GATTCAAGGA GTGAAGGCCA TGGTACCGCA NAGGCGGGTC 120 TTCCGGGCGT GCACCGAATT TTGCATCGGA TGAAAACCTT GGTCATCAGC A ATGGGGGC 180 TGGGTTCCAC CAAGGGAAAA GTTCGAATGC AAGTACCAGT GAGTCCCATG AGAGGGACTT 240 GATCATGCAG AGGTATGATC GAAGCAGCTG GAGANTTGGA CTGCTCCANA GCTCATATTC 300 GCTTAAGGGA GCCCGACAAG TCAGAGGACA AGGTCGAGTA AGCGAACGTT GCTACCCAAA 360 ATCAAGCATC AGTTANAATG GAGGTGGACT CANAGGAGTG CCACGGAAGA CATCTCTACT 420 GATTGTGAAG GAAAGGGATA CAGAGGCGAA NCGACGGATA NTANGGCCAT GGGCATGGCA 480 GCGCCATGGT ACCGCANANG CGGGACTTCC GTGCAAGTCA TTGATCCCTT GCTCTCACGG 540 AGGGANANCG CTTGGTCCTG AAAGGGGCCN AAGAAGTGGA CATGCANANG CAATCTCCAA 600 TTACCGAAAC ANGGCTGAAG GGCANAAGCC NANAAACTTC CTNAGAACGG TGTCAACAAT 660 TTCTCATCNA NATANCCGTA ATTAANGACT TCCGGTCATG CNANAATNCC CNACCNANGA 720 ACCAANCAGG CNTACTTGTG CTGTNCTTTG CTACCCNNTG AATNGCGCCN GGTTNATNGA 780 AAAAACGTCC TTCCCCAAGC AACCCTCTNT CAAAAAN 817 (2) INFORMATION FOR SEQ ID NO: 37: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 837 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U40 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37: ττττττχτττ TTTTTTTT GG GTATCAGGCA AAGATGGATA CTTCCAGGTT TGCTATTTAA 60 GAACCATAGT CAGTCTTATG AGGAGAGACC ATACGTAATA CAATCTTTAA GATGACAAAA 120 TCCATTCCTA CCAATACAAT GACCAAGCGA AAGGTACAAG GATAACGGGC AAAAGACTAA 180 ATTGCAACTA TATAAAATTC TGCTGCATTG TAGTAAATTC AACTGTGACG ACACGCCACC 240 AGGATGAAGT CTATCTATAA ACCCACTTAT TGCTAGATGG ACGGTGTAAC AGAGCTAAAA 300 CAGCAAAGTA GAATAAAAGA AACTGACAAC GCTTCCGCAT GCGCTCCACA TATGGAGCCA 360 GGCGGAGGTT GGGCAATTGG AGGAAATGAC NTTTCTGACG GTGTAACATA AAANAAATTT 420 GTAACACCGT CAAGTACGAT TTCCCATCCT TCGGGTTGTG GCGTTCTGCT ACTTGGCATT 480 ACGTTAACAT CGAGTGCCGA CTTGGCAAGA CAGGACCCCA GCATCAGAAG TCAGTGTGTC - 540 AACCAAGGAA GAAGGCTTGG CCCCAAAGCT GGAGGCCCGT GCGTAACTGG CTGAACCCCT 600 GCACCGGAAN GGGTGAAAAA AGCACCATTC AGAACAGGTC NCCTCCGACC TCCCAATTCC 660 AGTTCTTCCA CCNTGCTTTT GATCTGTGTC CACCTTTTTG TTACCTATTC CATCGAAGCN 720 TANGGATTTT TAAAAATCCC GAAGGAAAGG AANTTCCNAT AATTGGTGCN CCAAANAAGG 780 AAGATGGACN GAACTGTTGT TGGTTGCTCC TGTTCCTCCT TTGCAATGGA TTGGTCG 83 " !2) INFORMATION FOR SEQ ID NO: 38: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 799 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U108 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38: GGCACGAGGC AGCTCAATCG ACCGACTCCT CCTCCTCCTC CTCCTCCTCC TCTTGTTCTT 60 CTCATTCTAA TATAATTATC GCTTTTGGTG TGTACATCTT CTATAATTTG AGAGGATGGA 120 GGCAGGATTG ATGGCGAAAC AAGCAGCAGC GGTTGCGAAG CCGAACGCGT TCCCCGCCCG 180 GAGCCTAGGG TTCGGGAGCT CGGTCAGGGG CGGATCCGGG ACCAGCAGGA TCGGGTTCGA 240 GGCGCCCGCG AGCGTGGCAT GGAGGAAGCG GTCGATTCAG GTGGCCCGCC AAGGAGCCAT 300 TCGGTCGGAG GTGGTCGTGG AANANAAAGC ATCNCCGCCC AAAAAGGATA AGGCCGGTCC 360 GGGTCGGCTC TACGTGGGTC TGCCCTTGGA CGTGGTCTCC GACGGCAACG TCNTCAACCA 420 CGGCAAGGCC ATCGCCGCCG GTCTCCGCGC CCTCCGCCCT CCTCGGCGTT GATGGANTCN 480 AACTCCCCCA TCTCCTGGGG CGTTGGCGAT GGACTCCGGC GAATGGTCCT CCTACCTCCC 540 CCGTCNCCGC CATGGCGCGC GACNCCCGGC CTTCGCCTCC GCGTCTCCCT CCACCTCCAC 600 TGCCANCGCC GCCCTCGCCT CCCCCTCCCC NAATCGGTCG AATCCNCCGC CGCTAACAAA 660 CCCCGAAATC CTATTCNCTG AACGCNCCGG CCGCCNCCNG TTGCCCAAAN TNGCCTTNTT 720 CCTTTCCNCT TTGTTTNAAA AAAAACTCCC CNTTGTTCCT TCCAANCGGG NAGGAACCCC 780 CNAATGGAAA CCTTNCAAA 799 (2) INFORMATION FOR SEQ ID NO: 39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 786 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-U128 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39: GGCACGAGGA AACGTCTCTC CCTCTCTCCA CTTCTCACAC CCCCGCCCCC GACTCAGCGA 60 ATCGACATTC TTGGCGTGTC AAGGAAACCT TTCTTTTATT TGCGAAGGAA CATGAGCAAT 120 ACTGTTGGAC ACACCTTACC CCAGCAAGCT CTTTACCGGC CTTCTATCAA AGAAAATCAA 180 AGTAAAGCTC ATCAAGGAGT TTCTGCAAAC TTCTTGTGTG GAGTTCCATT AGGATCTAAG 240 GTGGAAAATG CAGTGTCATA CAGCTCTAGG TCACTGCTTT CAACCGGATC CCTGGGAAAG 300 AAACTCATCA AAGGAATC . ACCAAAGCAA AATCCAAGCA TAGTCACTAT GACCCCTCGT 360 GCTGTGTTGG CTGCANATCC TGCTTCAGAG CTAAGGAGAA AATTCAAGCT TGACACAAAT 420 TCTGAATTGG AGGTTGTTGT TCATGTCCCT ACTTCGGGGT CTCCTGTNCA GATTGAATTT 480 CAAGTNACTA ACAGCAGTGG CTACTTGGTG CTTCNTTGGG GTGCAATTCA TAATANAAGG 540 AATAACTGGT CACTTCCTTC TCGTCATCCT GATGGAACAA AAGTTTNCCN AAACCCGAAC 600 TCTCANGANA CCTTTTAAAA AATCTGGTTC CCCATCNTCC GTNAAAATGG AGATTGATGA 660 TCCTGAAATA CCAGCAGTNG AATTTCTCCT TANTTTTGNA ACAAATNCTC AAAAAAACCN 720 AAATTGGGTT TTTAAAACNA TTAATGGGTC CCNAAATTTC CNTGTTCCNN TTTTTTTANA 780 AACAAG 786 (2) INFORMATION FOR SEQ ID NO: 40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 804 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D4 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 40: GGCACGAGAG ATAGCTGAAG TGACGTGAGG TGACTTCGAT TAGATCGAGA GAAATTTCTT 60 TGTGATTTTT GTGGACAATT CTCAATGGCT GCTGTAACAA ANTCACAATT CATCTCGAAA 120 GGCTCATGCG CTGGCTATGG GGGTGTCGTC GACTCGGAGC CAAGGACCTT CCTAAACAGA 180 AGGGTTCTAC ACTTGAGGAA CCAAACCACC GCTTACGAAG GATTGAGATC TCGAAATGTG 240 GTTGATTTGA TTCAGATGCC GTCTAATGCC AAGGTGATTT CAAGGAAAAC TGTGAGGGGA 300 ACTCAGAATC CTAGTCGTAN ACCTTGGGCT GTTGTCATTT GTGGAAAAGG GATGAACATA 360 GTCTTTGTCG GCGCTGANAT GGCTCCCTGG AGTAAAACCG GANGGACTCG GTGATGTTCT 420 TGGAGGACTG CCACCGGCCA TGGCTGCAAA TGGACACAGA NTGATGACTA TAGCTCCACC 480 CTATGATCAG TACAAGGATG GGTGGGATAC AAATGTCCTG GCTGAATTAA AAGTTGGAAA 540 TGAAATTGAA ANAATCCGCT TTCTTCCACT GCTATAAAAA AAGAATTGAC AGGGTTTTCA 600 TTGATCACCT TTGTTTCTTG AAAANGTGTT GGGAAAAACT GGAAGAATGA AATTTTGGTC 660 CTNTCCCCGG AACTGATTTT NAAAAACANC CCCTAAANTT TANCCTTTTN TNCCCNGCCN 720 CTTTTGGAAA CTCCCNGGAT NCTGGTTTTT TNCCACCCCC NAAAANTTTC CTNGAACCTN 780 TGGGGGAAAN ττ--"-TTTTTT TTTT 804 (2) INFORMATION FOR SEQ ID NO: 41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 826 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D10 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41: GGCACGAGAG ATAGCTGAAG TGACGTGAGT GGACAATTCT CAATGGCTGC TGTAACAAAG 60 TCACAATTCA TCTCGAAAGG CTCATGCGCT GGCTATGGGG GTGTCGTCGA CTCTGAGCCA 120 AGGACCTTCC TAAACAGAAG GGTTCTACAC TTGAGGAACC AAACCACCGC TTACGAAGGA 180 TTGAGATCTC GAAATGTGGT TGATTTGATT CAGATGCCGT CTAATGCCAA GGTGATTTCG 240 AGGAAAACTG TGAGGGGAAC TCAGAATCCT AGTCGTANAC CTTGGGCTGT TGTCATTTGT 300 GGAAAAGGGA TGAACATAGT CTTTGTCGGC GCTGANATGG CTCCCTGGAG TAAAACCGGA 360 GGACTCGGTG ATGTTCTTGG AGGACTGCCA CCGGCCATGG CTGCAAATGG ACACAGAAGT 420 GATGACTATA GCTCCACGCT ATGATCAGTA CAAGGATGGG TGGGATACAA ATGTCCTGGC 480 TGANTTAAAA GTTGGAAATG AAATTGAAAG AATCCGCTTC TTCCACTGCT ATNAAANAAG 540 AATTGACAGG GTTTTCATTG ATCATCCTTT GTTTTCTTGA AAANGTGTTG GGAAAAACTG 600 GANGAATGAT ATNTGGTCCT GTCCCNGGAA CGGATTATTN AAAACCACCC NCTTAANATT 660 TACCCTTTTG TGCCCANGCA NCNTTTGGAA NCTCCCAAGG ATTCTGGATN TTTAACNACN 720 NCCNAATACT TTTCCTGGAA CCATTTNGGG GANAANNTTG TTNTTTGTTG CNAATTAATT 780 GGCNCCCTGG TCCTCCTNCC TGCTACTTAA AAATTTTNTT TCNTNT 826 (2) INFORMATION FOR SEQ ID NO: 42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D13 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 42: GGCACGAGCT GAGGGAGTGA GGGAAAGAGA TAGCTGAAGT GACGTGAGGT GACTTCGATT 60 AGATCGAGAG AAATTTCTTT GTGATTTTTG TGGACAATTC TCAATGGCTG CTGTAACAAA 120 GTCACAATTC ATCTCGAAAG GCTCATGCGC TGGCTATGGG GGTGTCGTCG ACTCGGAGCC 180 AAGGACCTTC CTAAACAGAA GGGTTCTACA CTTGAGGAAC CAAACCACCG CTTACGAAGG 240 ATTGAGATCT CGAAATGTGG TTGATTTGAT TCAGATGCCG TCT ATGCCA AGGTGATTTC 300 AAGGGAAAAC TGTGAGGGGA ACTCAGAATC CTAGTCGTAG ACCTTGGGCT GTTGTCATTT 360 GTGGAAAAGG GATGAACATA GTCTTTGTCG GCGCTGANAT GGCTCCCTGG GAGTAAAACC 420 GGAGGACTCG GTGATGTTCT TGGAGGACTG CCACCGGGCC ATGGCTGCAA ATGGACACAG 480 AAGTGATGAC TATAGCTCCA CGCTATGATC AGTACAAGGA TGGGTGGGAT NCAAATGTCC 540 TGGCTGAATT AAAAGTTGGG AAATGAANTT GAAAGAATCC GCTTCTTCCA CTGCTATAAA 600 ANAAGAATTG ACAGGGTTTT CATTGATCAC CTTTGTTCTT GAAAAGGTGT GGGGAAAAAC 660 TGGAAGAATG ATATTTGGTC CTGTCCCNGG ACTGATTTNA AAACACCACC TAAAATTACC 720 TTTTGTNCCN GCNNCTTTGG AACCNCCCNG GATCTNGATN TTANCACCCC CAANTCTTAT 780 CCTGGAACTT TTGGGGNAAA NTTTGTNTTT GTT 813 (2) INFORMATION FOR SEQ ID NO: 43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 805 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA Cvii) IMMEDIATE SOURCE: (B) CLONE: U-D66 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 43: GGCACGAGAA GAGATAGCTG AAGTGACGTG AGTGGACAAT TCTCAATGGC TGCTGTAACA 60 AAGTCACAAT TCATCTCGAA AGGCTCATGC GCTGGCTATG GGGGTGTCGT CGACTCGGAG 120 CCAAGGACCT TCCTAAACAG AAGGGTTCTA CACTTGAGGA ACCANACCAC CGCTTACGAA 180 GGATTGANAT CTCGAAATGT GGTTGATTTG ATTCAGATGC CGTCTAATGC CAAGGTGATT 240 TCAAGGAAAA CTGTGAGGGG AACTCACAAT CCTAGTCGTA NACCTTGGGC TGTTGTCATT 300 TGTGGAAAAG GGATGAACAT AGTCTTTGTC GGCGCTGAGA TGGCTCCCTG GANTAAAACC 360 GGAGGACTCG GTGATGTTCT TGGAGGACTG CCACCGGCCA TGGCTGCAAA TGGACACACA 420 GTGATGACTA TAGCTCCACG CTATGATCAG TACAAGGATG GGTGGGATAC AAATGTCCTG 480 GCTGANTTAA AANTTGGAAA TGAAATTGAA AAGANTCCGC TTCTTCCACT GCTATAAAAN 540 AAGAATTGAC AGGGTTTTCA TTGATCATCC TTTGTTTCTT GAAAAAGTGT GGGGAAAAAC 600 TGGGAAGAAT GATATNTGGT CCTGTCCCNG GAACTGATTA TAAAACACCA GCTAANATTA 660 CCTTTTGTTG CCAGCAACTT TGGAACCCCN AGGATTCTGG ATNTTACAAC ACNAATACTA 720 TTCTGGAACA TTTGGGGAAA AA.TTGTTTTT GTTNCCAATG ANTGGCNCAC TGGTCCCNTC 780 CCTNCCTACT TAAAAANTTT TTTCC 805 (2) INFORMATION FOR SEQ ID NO: 44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 790 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-Dlll (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 44: GGCACGAGAA GAGATAGCTG AAGTGACGTG AGTGGACAAT TCTCAATGGC TGCTGTAACA 60 AAGTCACAAT TCATCTCGAA AGGCTCATGC GCTGGCTATG GGGGTGTCGT CGACTCTGAG 120 CCAAGGACCT TCCTAAACAG AAGGGTTCTA CACTTGAGGA ACCAAACCAC CGCTTACGAA 180 GGATTGAGAT CTCGAAATGT GGTTGATTTG ATTCAGATGC CGTCTAATGC CAAGGTGATT 240 TCGAGGAAAA CTGTGAGGGG AACTCAGAAT CCTAGTCGTA GACCTTGGGC TGTTGTCATT 300 TGTGGAAAAG GGATGAACAT AGTCTTTGTC GGCGCTGANA TGGCTCCCTG GAGTAAAACC 360 GGAGGACTCG GTGATGTTCT TGGAGGACTG CCACCGGCCA TGGCTGCAAA TGGACACAGA 420 GTGATGACTA TAGCTCCACG CTATGATCAG TACAAGGATG GGTGGGATAC AAATGTCCTG 480 GCTGAGTTAA AAGTTGGAAA TGAAATTGAA AGANTCCGCT TCTTCCACTG CTATAAAAGA 540 AGANTTGACA GGGTTTTCAT TGATCATCCT TTGTTTCTTG ANAAGGTGTG GGGAAAAACT 600 GGAAGAATGA TATATGGTCC CTGTCNCAGG AACNGATTAT GAANACAACC AGCTAAGATT 660 AACCTTTTGT TGCCAGCACT TTGGGAACCN CAAGGATCCT GGATCTTAAC AACNCAANTC 720 TATTCTGGAC CATATGGGGA CAAATTGTTT TGTTGCAAAT GATGGCACCT GGTCCNNTCC 780 ATGCTACTTA 790 (2) INFORMATION FOR SEQ ID NO: 45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D112 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 45: τττττ , τττττ TTTTTTTTTG GAGCATAATA GCAATTTATT CAGGAGGATC AAACTCAAAC 60 ACTATAACAT AGACAGTAGT CACTTTCAGG GAGTGGCCAC ATTTTCCTTT GCAAGAGGAG 120 CTATTTCATC AGCATCGATG CCGGCTTCAC TACCAGCAGC TCCTAAGCTT AGGANAAATT 180 GCTCCCACTT CTTAGCAGGT CCCTTCCAGG AGAGGTCTTG AGTCATGCAG TTCTGTATCA 240 TCTCAGCAAA TGCAGGTGTG CCGTAAACTT TAAGGGCCCT TTTCACTGTC TGAATGACTT 300 TTTGTACATC ATCTTTATCG ACAACATCAC AATCAGGATT GAAGGGACCC ATATGAAACC 360 CTGTGATGCC TTCTATGACA GTGTCAGCAA GTCCACCAGT TGTTGCGCAC ATGGGGGGAA 420 TTCCATATCG CATGCCCTGA AAGCTGGATA AGGCCACAAG GTTCGAATCT GCTGGTAAGA 480 NCAAGAAGAT CTGCTCCAGC CATGATTTCA TGANCCAAAG GTGCATTAAA CTTCATATGT 540 GCTCTCACTT TATTGGGAAA CATATCTTCA AGTTGTGCAA GCTGACGCTC CAACANCTTC 600 TCCCANTACC AAGCACTATC ACTTGAACAT TTTCATCAAT GAATTCAGGA ATAACTGCNG 660 CGAAAATATC CGAACCTTTC TGCTCTCTAA TCTCCCTATA AAGGNTATAA CAGGGATTTC 720 CNGTCAACAG GTTAACCAAA TTCAACTTGT TAGGNTTCCC TTAATCCAAA GGTTTGCACC 780 CNNCCCATTT GTTGCTCNTA ATTGANAAAA ATT 813 (2) INFORMATION FOR SEQ ID NO: 46: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 808 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D39 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 46: GGCACGAGCG AGTTTTTTTT TTTTTTTTTT GCTGTGATGA TATTATGCAT CTTATTAGTT 60 GGTTGCACCA CTTCTATTAC ATCACTCAAC AGTATTAGCC CAAGANAAGG CTAATACCTT 120 CNCATCACTC TTGACATCTT ATTTGTATTG TAGGANACTA GTATATATAT CTATATGCGT 180 GGTTGCCTGC ACNAGGGCGG CTTATTAGTG GCGTCAGTGA TCTCAAGGGC AGAANACAAC 240 CCTGTANTTG GTGCCGCCGG GGCAGGTGAA GGTGCTCGTC TGATCGTCCT TGGGATANCT 300 GTAGGCGTCG GGGCAGTTTC TCTTGAANAA CCGGGAGTAT TCGGTCGGGC TGCAGCTGCC 360 GGAGTTGCAA CAGTACTGGT CCGTCTTGAA CACGGT.GCAG GGGTTGTTGC ANCCGCCCGG 420 CGCCTTCANC GCCCCGGGGC ACTGCCCGTT GATNTCCGCC GCGCACCGGA TGCCGCGGCA 4 80 NCCGCCTGAC NTGGGGCTNA ATCCCATTGG CACGTTGAAA CCGTCNACCA AGGGAAATTT 540 CAAAAAATCC AAGTTGTTGA ACTGGTTGAA CGCCAACTCC NCCAGGGTNT TGGGCCGGTT 600 GCCNTACCCC GTGCATNACA ACACCCCGCC GCANTCCCCT TCTGGCACCG CCGCNTCCGC 660 TCCCCTCAAA AGAACACCCG TGCGGCCCAA AATCCGGNCC CCCGGTGGTT CCCCCTTTCN 720 CTTTAANGGT CCCCAATGCC CCTGNTTTAA CTGNCTCCCC CCCANGCNCG GCCCCGGCCC 780 ACNCTTTNTT AAAACATCNG TNCCNAAT 808 ( 2 ) INFORMATION FOR SEQ ID NO : 47 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 809 base pairs ( B ) TYPE : nucleic acid ( C ) STRANDEDNESS : single ( D ) TOPOLOGY : unknown ( i i ) MOLECULE TYPE : cDNA ( vi i ) IMMEDIATE SOURCE : ( B ) CLONE : U-D50 ( xi ) SEQUENCE DESCRI PTION : SEQ I D NO : 47 : GGCACGAGTG TGCCTAACAG AGAAAGAGAG ACCGATAGCC TCCTCATTCA CTATGGCGAT 60 CCGATCGCCA GCTTCGCTGC TGTTATTTGC GTTTCTGATG CTTGCGCTCA CAGGAAGACT 120 GCAGGCCGGG CGCAGCTCGT GCATTGGCGT CTACTGGGGA CAAAACACAG ACGAGGGAAG 180 CTTAGCAGAT GCTTGTGCCA CAGGCAACTA CGAATACGTG AACATCGCCA CCCTTTTCAA 240 GTTTGGCATG GGCCAAACTC CANAGATCAA CCTCGCCGGC CACTGTGACC CTCGGAACAA 300 CGGCTGCGCG CGCTTAAGCA GAGAAATCCA GTCCTGCCAG GAGCGTGGAG TCACGGTGAT 360 GCTCTCCATC GGAGGTGGCG GGTCTTATGG CCTGAGTTCC ACCGAAGACG CCAAGGACGT 420 GGCGTCATAC CTCTGGCACA GTTTCTTGGG TGGTTCTGCT GCTCGCTACT CTCGACCCCT 480 CGGGGATGCG GTTCTGGATG GCATANACTT CAACATCNCC GGAGGGAGCA CAGAACACTA 540 TGATGAACTT GCCGCTTTCC TCAAGGGCTA CNACGAACAG GAAGCCGGAA CGAAAAAANT 600 TTTACTTGAA TGCTGCTCCC NCANTGTCCT TTCCCGGATT ACTGGCTTGG CACCCACTCA 660 NAAAANATCT CTTCCNACTT CCNTGTGGGT TGCANTTCCT CCAANAACCC TTCCTTGCCN 720 TTTCTCCCCA AAACCTATCC ATCTTGCAAT TCCTTTCACA AATTGGGTCN TNTCCNTCCC 780 NGCCCCNAAA ACTTTTTCCC TNGGGCTCC 809 (2) INFORMATION FOR SEQ ID NO: 48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 809 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D86 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 48: GGCACGAGCC ACAAGCTTGC CTTTGGTTGT GCCTAACAGA GAGAGAGAGA GAGACAGACC 60 GATAGCCTCC TCATTCACTA TGGCGATCCG ATCGCCAGCT TCGCTGCTGT TATTTGCGTT 120 TCTGATGCTT GCGCTCACAG GAAGACTGCA GGCCCGGCGC AACTCATGCA TTGGCGTCTA 180 CTGGGGACAA AAGACAGACG AGGGAAGCTT AGCAGATGCT TGTGCCACAG GCAACTACGA 240 ATACGTGAAC ATCGCCACCC TTTTCAAGTT TGGCATGGGC CAAACTCCAG AGATCAACCT 300 CGCTAGCCAC TGTGACCCTC AGAACAACGG CTGCGCGCGC TTAAGCAGCG AAATCCAGTC 360 CTGCCAGGAG CGTGGAGTCA AGGTGATGCT CTCCATCGGA GGTGGCGGGT CTTATGGCCT 420 GAGTTCCACC GAAGACGCCA AGGACGTGGC GTCATACCTC TGGCACAGTT TCTTGGGTGG 480 TTCTGCTGCT CGCTACTCTC GACCCCTCGG GGATGCGGTT CTGGATGGCA TAAACTTCAA 540 CATCNCCGGA GGGAGCACAG AACACTATGA TGAACTTGCC GCTTTCCTCA AGGGCTACAA 600 CGANCAGGAA GCCGGAACGA AAAAAGTTCA CTTGAATGCT GCTCCCCANT GTCTTTCCCG 660 GATTACTGGC TTGGCAACGC NCTCCAAAAC AAATCTCTTC CACTTCCTGT GGGTGCANTC 720 CTCCACAAAC CTTCNTGCAT TCTCCCCAAA CCTATCAATC TTGCNAAATG CGTTCAACAA 780 TTNGGGTCTT TTCATCCCCG CCCAAAACT 809 (2) INFORMATION FOR SEQ ID NO: 49: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 785 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D90 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 49: GGCACGAGGA GAGACCGATA GCCTCCTCAT TCACTATGGC GATCCGATCG CCAGCTTCGC 60 TGCTGTTATT TGCGTTTCTG ATGCTTGCGC TCACAGGAAG ACTGCAGGCC GGGCGCAGCT 120 CGTGCATTGG CGTCTACTGG GGACAAAACA CAGACGAGGG AAGCTTAGCA GATGCTTGTG 180 CCACAGGCAA CTACGAATAC GTGAACATCG CCACCCTTTT CAAGTTTGGC ATGGGCCAAA 240 CTCCAGAGAT CAACCTCGCC GGCCACTGTG ACCCTCGGAA CAACGGCTGC GCGCGCTTAA 300 GCAGAGAAAT CCAGTCCTGC CAGGAGCGTG GAGTCACGGT GATGCTCTCC ATCGGAGGTG 360 GCGGGTCTTA TGGCCTGAGT TCCACCGAAG ACGCCAAGGA CGTGGCGTCA TACCTCTGGC 420 ACAGTTTCTT GGGTGGTTCT GCTGCTCGCT ACTCTCGACC CCTCGGGGAT GCGGTTCTGG 480 ATGGCATANA CTTCAACATC GCCGGAGGGA GCACAGAACA CTATGATGAA CTTGCCGCTT 540 TCCTCAAGGC CTACAACGAG CAGGAAGCCG GAACGAAAAA AGTTTACTTG AATTGCTGCT 600 CCGCANTATC CTTTCCNGAT TACTGGCTTG GCAACNCNCT CCAAAAAANA TCTCTTCCAC 660 TTCCTGTTGG GTGCAGTTCT TCCAANAACC CNTTCNTGCC ATTTCTCCCC AAAACGCTTT 720 CCATCTTTGC AAATGCCTTT CAACAATTGG GGTCTTGTTC CNNCCCTGCC CCAAAAACTG 780 TTCCT 785 ( 2 ) INFORMATION FOR SEQ ID NO : 50 : (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 813 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D93 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50: GGCACGAGCG ATAGCCTCCT CATTCACTAT GGCGATCCGA TCGCCAACTT CGCTGCTGTT 60 ATTTGCGTTC CTGATGCTTG CGCTCACGGG AAGACTGCAG GCCCGGCCCA GCTCATGCAT 120 TGGCGTCTAC TGGGGACAAA ACACCGACGA GGGAAGCTTA GCAGATGCTT GTGCCACAGG 180 CAACTACGAT TACGTGAACA TCGCCACCCT TTTCAAGTTT GGCATGGGCC AAACTCCAGA 240 GATCAACCTC GCCGGCCACT GTGACCCTCG GAACAACGGC TGCGCGCGCT TGAGCAGCGA 300 AATCCAGTCC TGCCAGGAGC GTGGCGTCAA GGTGATGCTC TCCATCGGAG GTGGCGGGTC 360 TTATGGCCTG AGTTCCACCG AAGACGCCAA GGGAAGTAGC GTCATACCTC TGGCACAGTT 420 TCTTGGGTGG TTCTGCTGCT CGCTACTCGA GACCCCTCGG GGATGCGGAA CTGGATGGCA 480 TANACTTCAA CATCGCCGGA GGGAGAACAG AACACTATGA TGAACTTGCC GCTTTCCTCA 540 AGGCCTACAA CGANCAGGAA GCCGGAACNA AAAAAGTTCA CTTGAATTGC TGCTCCGCAG 600 TGTCCTTTCC CGGATTACTG GCTTGGCAAC GCACTCAGAA CANATCTCTT CNACTTCCTG 660 TGGGTGCAAT TTCCTCCACA ANCCTTCCNT GCCATTTCTC CCAAAAAGCT ATCNATCTTG 720 CAAATGCGTT CNACAATTGG GTCTTTTCCA NCCTGCNCAA AACTGTTCCT TGGGCTCCCG 780 CTGCCCCTGA AGGTGCNCCA ATTGGTGGCT NCT 813 (2) INFORMATION FOR SEQ ID NO: 51: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 819 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D61 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51: GGCACTCAGG AACTCCAACA TCCAAGTCCT GTTGGATGTC CCCCGATCCG ACGTGCAGTC 60 ACTGGCCTCC AATCCTTCGG CCGCCGGCGA CTGGATCCGG AGGAACGTCG TCGCCTACTG 120 GCCCAGCGTC TCCTTTCGAT ACATAGCTGT CGGAAACGAG CTGATCCCCG GATCGGATCT 180 GGCGCAGTAC ATCCTCCCCG CCATGCGCAA CATCTACAAT GCTTTGTCCT CGGCTGGCCT 240 GCAAAACCAG ATCAAGGTCT CGACCGCGGT CGACACGGGC GTCCTCGGCA CGTCCTACCC 300 TCCCTCCGCC GGCGCCTTCT CCTCCGCCGC CCAGGCGTAC CTGAGCCCCA TCGTGCAGTT 360 CTTGGCGAGT AACGGAGCGC CGCTCCTGGT CAATGTGTAC CCTTATTTTA GCTACACCGG 420 CAACCCGGGA CAGATCTCGC TGCCCTACGC CCTGTTCACG GCCTCCGGCG TCGTCGTGCA 480 GGATGGGCGA TTCANCTATC AGAACCTGTT CGACGCCATC GTCGACGCGG TCTTCGCGGC 540 GCCTGGAAAA AATGGGAAGG GCGAACGTGG CGGTGGTGGT GTCCGAAAAC GGGTGGCCGT 600 CCGCGGGCGG AAGAACCCNA AACNAACACC ANCAACNCCG CCGGAANTTT CAACCANAAC 660 TTGATCAGGC ATGTTNGGCG GAAGAACCCC AAGGAAAACC AGGGAAGGAA ATCAAGGCTT 720 CNTNTTCCAA AANTTTCCAC CAAAAACCNA AAGGTGGANG GATCCAACAA AACTTTGGCC 780 TGTTTTATCC CAANNAACAN CCCNTCTTAC CNAATAACT 819 (2) INFORMATION FOR SEQ ID NO: 52: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 794 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D63 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 52: GGCACGAGGG ATTATAAAAA GAAACTCGCA CTCAGCCAGA TTGACTCAAG CATGAAGGTT 60 GCATCTTTCG AATGTTCCTT TTTTTTCCAA CATCTTTTGA ATGTTACATG ACAATCATGA 120 CGCCGTGCAT ACAATGATAC CTCTGATAGG GTTGAATGCA CAAACCACTC ATCGTGCTAT 180 ATTGCAAGTG CTTATGTTTG TATTCATTTT CTTATAATTT CAATGTGAGA GTTACCTTAG 240 GTTCATATTA NATTTATATA ATATTGGGCA TAGATTTTTT TGTCATTGTT TGTGATCTGC 300 AATTTCATTA GGTCATCGAC CATATTATGG TAAATTTAAT TTACTAAATT ATATCTTGAG 360 TTTATTGCTT TGCATATTTT GTGCTGGAAA CCATGCCAAT CCAACTCCTT TGCAAAACTT 420 AAGATAATTA AAGGAGATAT TGAGGATGCC GACTGCTTTA GGTTATTTGC ATTANACAAT 480 AAACGCCCGT GAAATGGAAA TCATGCAAAG CTTTGCATGG AGAATATGTT NATAGAGATA 540 GACATGAAGA CATGAATATG CTTTCTTGAT GAACAACGAT GTTCACNTGT TTACTGCATG 600 CATGATACAT CCAATGTTCT AGGCTTGTTG GCAATCATTT TATTCNAAAA ATTGTCTGTT 660 TCTCTNCCNC CATAAGGTTA GCTTGTGGAA AATGTTCAAC TTTGGGCNCA NATGATCCGT 720 TTTAGCAAAA TCCCNCCATT CCTATTTTTT TCCGGAATCC NNTTGGNAAA ANATCCTNAC 780 ATACTATTTC CACA 794 (2) INFORMATION FOR SEQ ID NO: 53: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 798 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA ( ii) IMMEDIATE SOURCE: (B) CLONE: U-D65 (x ) SEQUENCE DESCRIPTION: SEQ ID NO: 53: GGCACGAGGC CCGTAGAGCG AGTGCTGAGA TGGCTTTGAG GGCCTTCTTC CCCCTCTGCA 60 TCGCTCTCGT GGTGACGCTC TCACCTCTGT GTGATGCCAC TTCGCCTTAC TACACCATCA 120 CACCGCCCAC CCCCGTGGCC AAGCCGCCTT CAGTTGAACC ACCACCCTAC CACGGCCCTC 180 CGACGACCCA CCCTAAGCCA CCGAGTCATG GTGGCCAACC TCCGTCCCAC CATCACCCAA 240 CACCAATCTA CGGTGCACCC CCTCCGCAAC ACCACCACCA CCACCAACAC CACCACCAAC 300 CTGCACCACC AACTCACGCA GAACACCCTC CGTACTACCA CATGCCTTCC CCGCCGCCGC 360 ATGGCCAGCA CCCGTCACCA CCGTCACATG ATTATCCCGT ACCTCCTGCT CACAAGCCCC 420 GAACTCCGCC GCCGGTTTAC AAGTCTCCAC CACCGACCCA CCGTCCTTAC CCTCCATCGA 480 CGCCACCCCA CCATCCGACG CACCCGCCTT CTCAGCCGAC GCCGTCATAC AAGGCCCCGC 540 CACCATACAA GAACATCCCT GANCATCTCC ACCGCCGCGT CACTATCATT CTCCGTCTTC 600 ACCACCANCA CAACCACCAT NCAAATAGTC TCGTTTGCAT CTCTCCGTTG AANATGAACC 660 AATGTCNTTT AATAACGATC AGGGTTTCAA ATAAAAACNA ATTTCCGCCA TTGTAATGCT 720 ATGGTTGTTC TCTCTGCTTC CCGGGGAAAG TTTCTTGGGT CATNTTAACC NCCTCCTAAT 780 GTTCNGCTCT TNNTANAA 798 (2) INFORMATION FOR SEQ ID NO: 54: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 824 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D84 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54: GGCACGAGCG CCCACCCCCG TGGCCAAGCC GCCTTCAGTT GAACCACCAC CCTACCACGG 60 CCCTCCGACG ACCCACCCTA AGCCACCGAG TCATGGTGGC CAACCTCCGT CCCACCATCA 120 CCCAACACCA ATCTACGGTG CACCCCCTCC GCAACACCAC CACCACCACC AACACCACCA 180 CCAACCTGCA CCACCAACTC ACGCAGAACA CCCTCCGTAC TACCACATGC CTTCCCCGCC 240 GCCGCATGGC CAGCACCCGT CACCACCGTC ACATGATTAT CCCGTACCTC CTGCTCACAA 300 GCCCCGAACT CCGCCGCCGG TTTACAAGTC TCCACCACCG ACCCACCGTC CTTACCCTCC 360 ATCGACGCCA CCCCACCATC CGACGCACCC GCCTTCTCAG CCGACGCCGT CATACAAGGC 420 CCCGCCANCA TNCNAGACCA TCCCTTGAGC ACTTCTCCAN CGNTTGCGTT TATTATCATT 480 CTCCGTCTTC ACCACCACCA CCACCACCAT ACAAATAGTC TCGTTTGCCA TCTCTCCGTT 540 GAAGATGACG CAGTGTCGTT TTAGTAGCGA TCAGGGTTAC CAATAAGAAC GATGTTGCCG 600 CCATTGTAAT GGCTATGGTT GTTCTCTCTG CTTTTCCGGG GGAAGGTTCT TTGGGTTCAT 660 GTTAAACCTC TCTCTTAAAT GTTCATGCAT CTTATTATAA ACNAAAATTG GCCATTTNNN 720 NNNTNTNNTN NNNNNTTNNN NNNNNAAAAC TCNAAAAATA TTTTTAAAAA CGGGCGGGGG 780 GCCCATCNAT TTTCCNNCCC GGGTNGGGGN TCCCAGNTTA TTNT 824 (2) INFORMATION FOR SEQ ID NO: 55: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 797 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D75 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 55: GGCACGAGGG TTACGATCGT CATGTTGGGG GTGTTCAGCG GGGNAGGTGG TGGAGGTGCC 60 GGCGGAGCTG GTGGCCGCCG GCAGCAGGAC GCCGTCCCCT AAGACACGAG CGTCGGAGCT 120 GATCAACCGA TTCCTCGGGA GCTTCGCTCC CGCGGTGTCG ATCCAGATCG GGGACTTGGG 180 ACACCTCGCC TACTCCCACG CCAACCAGTC CCCCTTCGCT CCCAGGTTGT TTGCAGCGAA 240 GGACGANATT TACTGCCTCT TCAAGGGAGT GCTGACCAAC CTGGGCAGCT TGAGGCAGCA 300 GTATGGGCTT TCCAAGAGTG CCGACNAGGT GGTGCTGGTC ATCGAAGCCT ACAAGGCCCT 360 CCGTGACCGA GCTCCCTATC CTCCCAGCTT CATGCTCGCA CACCTTACTG GCAACTTCGC 420 CTTCGTGCTC TTCGACAAGT CCACATCATC CATCCTTGTT GCATCTGACC CANATGGAAN 480 ANTACCCTTG TTCTGGGGGA TCACTGCANA TGGATGCCTT GCCTTTGCTG ACNATCTANA 540 CTTGCTGAAG GGATCGTGCG GGAAGTCACT TGCACCATTC CCTGAAGGAT GTTACTATTC 600 CAATGCCTTG GGGGGCCTGA AAANCTATGA AAACCCCAAC ACAAGGTGAA CTGCTGTTTC 660 TTGAANATGA AGAANAATTN TTTGTGCCNC TTTCAAGGTG GAANGATCTG CCNTTCTTGC 720 GGNAACCCCC TAATCAAGGA ACATCTTCCA ATGTTTGGGG CNAATGATCC TCTTAAAATC 780 CTAAAATTCT TGGAATT 797 (2) INFORMATION FOR SEQ ID NO: 56: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 804 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D83 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56: GGCACGAGCT TCCACACAGG TGAGGGTAAG TCGGTTACGA TCGTCATGTT GGGGGTGTTC 60 AGCGGGGAGG TGGTGGAGGT GCCGGCGGAG CTGGTGGCCG CCGGCAGCAG GACGCCGTCC 120 CCTAAGACAC GGGCGTCGGA GCTGATCAAC CGATTCCTCG GGAGCTTCGC TCCCGCGGTG 180 TCGATCCAGA TCGGGGACTT GGGACACCTC GCCTACTCCC ACGCCAACCA GTCCCCCTTC 2 40 GCTCCCAGGT TGTTTGCAGC GAAGGACGAN ATTTACTGCC TCTTCAAGGG AGTGCTGACC 300 AACCTGGGCA GCTTGAGGCA GCAGTATGGG CTTTCCAAGA GTGCCGACNA GGTGGTGCTG 3 60 GTCATCGAAG CCTACAAGGC CCTCCGTGAC CGANCTCCCT ATCCTCCCAG CTTCATGCTC 420 GCACACCTTA TTGGCAACTT CGCCTTCGTG CTCTTCGACA AGTCCACATC ATCCATCCTT 480 GTTTGCATCT GACCCANATG GAAAAATGCC CTTGTTCTGG GGGATCACTG CANATGGATG 540 CCTTGCCTTT GCTGACNATC TANACTTGCT GAAGGGATCG TGCGGGAANT CACTTGCACA 600 TTCCCTGAAN GATGTTACTA TTCCAATGCC TTGGGGGGGC TGAAAANCTA TGAAAACCCA 660 ANCACAAGGT GACTGCTGTC TTGAANATAA AGAAAAATTT TTTGTGCCCC TTTCAAGGTT 720 GAANGATCTG CATTCTTGCG GCACCCCCTN ATCNAGGAAC NTCNTNCCAA TGTTGGGGCA 780 AATATCCTCT TTAAAANCAT AAAA 804 (2) INFORMATION FOR SEQ ID NO: 57: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 803 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: cDNA (vii) IMMEDIATE SOURCE: (B) CLONE: U-D64 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 57: GGCACGAGGG ATTATAAAAA GAAACTCGCA CTCAGCCAGA TTGACTCAAG CATGAAGGTT 60 GCATCTTTCG AATGTTCCTT TTTTTTCCAA CATCTTTTGA ATGTTACATG ACAATCATGA 120 CGCCGTGCAT ACAATGATAC CTCTGATAGG GTTGAATGCA CAAACCACTC ATCGTGCTAT 180 ATTGCAAGTG CTTATGTTTG TATTCATTTT CTTATAATTT CAATGTGAGA GTTACCTTAG 240 GTTCATATTA GATTTATATA ATATTGGGCA TAGATTTTTT TGTCATTGTT TGTGATCTGC 300 AATTTCATTA GGTCATCGAC CATATTATGG TAAATTTAAT TTACTAAATT ATATCTTGAG 360 TTTATTGCTT TGCATATTTT GTGCTGGAAA CCATGCCAAT CCAACTCCTT TGCAAAACTT 420 AAGATAATTA AAGGAGATAT TGAGGATGCC GACTGCTTTA GGTTATTTGC ATTANACAAT 480 AAACGCCCGT GAAATGGAAA TCATGCNAAG CTTTGCATGG AGAATATGTT NATAGANATA 540 GACATGAAGA CATGAATATG CTTTCTTGAT GAACAACGAA GTTCACNTGT TTACTGCATG 600 CATGATACAT CCAATGTTCT AGGCTTGTTA GGCAACCTTT ATCCAAAAAA TTGTCTGTAC 660 TCTCCCCCAT AAGGTAAGCC TGTGGAAAAT GTTCACTTTG GGCCNNATGA TCAGTTTANC 720 CGAAAATCCC CCTTCNTTAT TTGTTTTCTG AAACNCNTTG GAAANANATT CCTTACATAC 780 CTTTTTCACN NANATNTTGA ACC 803

Claims: CLAIMS 1. A method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence selected from the sequences depicted as SEQ ID Nos 1 -57, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics. 2. A method according to claim 1 wherein the polynucleotide is obtained from the cDNA library having the NCIMB Accession Number 40814. 3. A method of modulating the ripening or tissue senescence process in plants of the genus Musa comprising inserting into plant material at least one polynucleotide sequence or a fragment thereof, obtainable by hybridisation, from the cDNA library having the NCIMB Accession Number 40814, by the use of at least one of the sequences depicted as SEQ ID Nos 1-57 as oligonucleotide probes, said hybridisation being conducted at a temperature from 60°C to 65°C in 0.3 strength citrate buffered saline containing 0.1% SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1% SDS, regenerating said plant material and selecting from the transformed regenerants, plants with modulated ripening or tissue senescence characteristics. 4. A method according to claim 1 or 2 or 3, characterised in that the said polynucleotide modulates the production of pectate lyase. 5. A method according to claim 4 in which the polynucleotide sequence comprises at least one of the sequences depicted in the sequence listings as SEQ ID Nos. 13-18. 6. A method according to any preceding claim wherein the plant material is transformed using the Agrobacteήum, microparticle bombardment, fibre mediated or direct insertion method. 7. Plants, their progeny and seed and material obtained from said plants, produced according to a method as claimed in claims 1 to 6. 8. A vector functional in plants comprising a promoter region which is operable in plant cells, a polynucleotide sequence defined in claims 1 to 3 and a transcription termination sequence. 9. A vector according to claim 8 wherein the promoter is constitutive, developmentally regulated or switchable. 10. A vector according to claim 9 wherein the promoter is tissue specific or organ specific. 1 1. A genetically modified banana produced via the method according to claims 1 to 6 having altered fruit characteristics when compared with a banana which is not transformed with at least one of the polynucleotide sequences described in claims 1 to 3. 12. A method of controlling plant pathogens comprising the application of an anti- pathogenic agent to plants, characterised in that plants to which the said agent is applied are plants according to claim 7. 13. A polynucleotide associated with fruit ripening selected from SEQ ID Nos. 1 -57. 14. A fruit of a plant of the genus Musa having a retarded ripening phenotype generated by inserting into the genome of the said plant at least one of the polynucleotide(s) as claimed in claim 13. 15. A method, polynucleotide, plant, its progeny, seed and material obtained from said plants and a banana substantially as hereinbefore described with reference to the accompanying drawings and figures.

Inventors: BIRD COLIN ROGER, MEDINA-SUAREZ ROSYBEL DE JESUS, SEYMOUR GRAHAM BARRON, BIRD, COLIN, ROGER, MEDINA-SUAREZ, ROSYBEL DE JESUS, SEYMOUR, GRAHAM, BARRON
Applicants: BIRD COLIN ROGER, MEDINA SUAREZ ROSYBEL DE JESUS, SEYMOUR GRAHAM BARRON, ZENECA LTD, BIRD, COLIN, ROGER, MEDINA-SUAREZ, ROSYBEL DE JESUS, SEYMOUR, GRAHAM, BARRON, ZENECA LIMITED
Application year: 1997
Main classification: C12N15
IPC codes: A01H5, C12N5, C12N9, C12N15