NOVEL WATER-SOLUBLE ANALOGUES OF 20(S)-CAMPTOTHECIN

Abstract: Novel water-soluble C-ring analogues of 20(S)-camptothecin having general formula (1). All the compounds of formula (1) are prepared from the compounds of general formula (2) having 20(S)-chiral carbon. The compounds of formula (1) possess potent anti-cancer and anti-viral properties. The invention also provides an alternate process for the preparation of known C-5 substituted compounds of formula (1).

Description: NOVEL WATER SOLUBLE ANALOGUES OF 20 (S) -CAMPTOTHECIN The present invention relates to novel water soluble C-ring analogues of 20(S) -Camptothecin having the general formula 1. In the above formula 1, R 1, R2, R3 and R4 are independently the same or different and represent hydrogen, hydroxy, lower alkoxy, aryloxy, lower alkanoyl, nitro, cyano, halo, carboxy, carbonyloxy, amino, substituted amino, lower alkyl, substituted lower alkyl or R 2 and R3 combined together represent - 0-(CH 2 ) n -0- where n=l or 2; R represents hydrogen, lower alkyl, substituted lower alkyl, lower aralkyl, hydroxymethyl, carboxymethyl, aminomethyl; substituted aminomethyl where the amino group is mono or disubstituted in which both substituents are independent or combined together to form 5 or 6 membered cyclic ring system containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur. The number of atoms in the cyclic ring system is 5 or 6. When R represents hydrogen, R 6 represents lower alkoxy; carbonyloxy; cyano; nitro; thio; thioalkyl; thioaryl; amide or amino group in which the amino group can be unsubstituted, mono or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of 3 to 8 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur; phenoxy, phenyl, benzoyl or benzyl where the phenyl group can be unsubstituted or substituted with mono, di or trisubstituents which may be selected from halogen, hydroxy, alkoxy, cyano, carboxy, nitro, amido, amino or substituted amino, thioalkoxy, phenyl, benzyl, lower alkyl, or substituted lower alkyl; cycloalkyl or cycloalkyl lower alkyl where the cyclic ring may be in the range of 3 embered to 7 membered ring system containing all carbon atoms; lower alkyl groups substituted with heterocyclic rings where the heterocyclic ring system having 3 to 7 atoms contains carbons with at least one heteroatom selected from oxygen, nitrogen and sulfur, the number of atoms in the ring (s) of the heterocyclic ring system is 3 to 7; lower alkanoyl; lower alkenyl; substituted lower alkyl or substituted lower alkenyl where the substituents can be halogen, hydroxy, benzyoxy, carbonyloxy, alkoxy, aryloxy, carboxyl, cyano, thio, thioalkyl, thioaryl, aryl, heteroaryl, nitro, amido or amino in which amino group can be unsubstituted or mono, or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of 3 to 8 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur; or R represents COOR' where R' represents hydrogen, lower alkyl, substituted lower alkyl, or lower aralkyl; or when R represents lower alkyl; lower alkenyl; lower alkanoyl; substituted lower alkyl; substituted lower alkanoyl; phenyl, benzyl or benzoyl in which the phenyl group may be unsubstituted or substituted; or lower alkoxycarbonyl; R represents phenyl; benzoyl; cyano; nitro; lower alkanoyl; or COOR' group in which R' represents hydrogen, lower alkyl, lower aralkyl or substituted lower alkyl. All the novel compounds of the formula 1 are 20 (S) -isomers, substantially free from the corresponding 20 (R) -isomer. All these compounds of the formula 1 are prepared from the compounds of the general formula 12 having 20 (S) -chiral carbon, where R 1 to R5 have the meaning described above 20 (S) -Camptothecin having the formula 2 is an alkaloid possessing strong antitumor activity, 2 It was first isolated from the plant Camptotheca acuminata by Wall and co-workers in 1966. However, its development as a potential drug for cancer treatment had been abandoned due to unacceptable side effects on humans and due to its low water solubility as well as high toxicity problems. Since the discovery of its mechanism of action as an inhibitor of topoisomerise I by Liu and co-workers in 1985 [L.F.Liu, etal., J. Biol . Chem. . 260 14873 (1985)], the research interest on camptotheein has once again taken momentum. To overcome this problem of low water solubility and high toxicity of camptothecin, over the last 30 years, several research groups all over the world have prepared and investigated a number of camptothecin analogues involving the modification of rings A-E or the introduction of a variety of substituents on all the five rings of camptothecin of the formula 2[ M.E.Wall et al., J.Med. Chem . , 3_6, 2689 (1993); R.P. Hertzberg et al. , J. Med . Chem . , 715 (1989); S.W.Sawada et al. , Chem. Pharm. Bull , 4_1(2), 310 (1993)] . Among the various camptothecin analogues prepared to date, only two of them namely, CPT-11 having the formula 3[ Chem. Pharm. Bull . , 3_9, 1446 (1991)] , 3 topotecan of the formula 4 [ J.Med. Chem . , 3 , 98(1991)] were introduced as anti-cancer drugs in the market recently. Another compound namely, 9-aminocamptothecin of the formula 5 [J. Med. Chem . , 2£, 2358 (1986)], 5 is currently undergoing phase III clinical trials. The extensively studied Structure-Activity Relationship(SAR) on camptothecin of the formula 2 [M.E.Wall et al. , J.Med.Chem. , 3_6, 2689 (1993)] has revealed that 20(S) -oc-hydroxy-δ-lactone (E-ring) moiety in camptothecin is essential for its activity. However, according to recent reports by Ejima et.al., replacement of hydroxyl group with an amino group at C- 20 position leading to a compound such as 7-ethyl-10- methoxycamptothecin derivative of the formula 6 [A. Ejima etal., Chem. Pharm. Bull . , 4_0(3), 683 (1992)] , 6 exhibited an increased in vivo antitumor activity than 20 (RS) -camptothecin of the formula 7. Also in another report ( Lawrence Snyder et.al., J". Org. Chem . , 5_9, 7033 (1994)], the 18-noranhydrocamptothecin analogue of the formula 8, 8 exhibited potent camptothecin like inhibition of topoisomerase I activity. Both these reports are contrary to the assumption that 20 (S) -α-hydroxy functionality in camptothecin is an essential feature for its biological activity. Based on the structure-activity results obtained for the camptothecin analogues so far prepared in the literature, it was established that the modification of substituents at C-9 and C-7 position of camptothecin of the formula 2 plays an important role in the enhancement of anticancer activity by imparting stability to the E-ring lactone [T.G.Burke etal., J.Med . Chem 37, 40(1994)] . It has also been recognized that the open form of the lactone moiety, namely, ' he Carboxylate form' is less effective therapeutically than the closed 'Lactone form ' [Hertzberg et al. , J. Med. Chem . , 3_2, 715(1989); J.M.Covey, C.Jaxel et al . , Cancer Research., 4_9, 5016 (1989); Giovanella et al. , Cancer Research . , 5_i, 3052 (1991)] . The recent studies by T.G.Burke et al. , on the stability of ' closed lactone form' of various camptothecin analogues in the presence of protein called 'Human Serum Albumin ' (HSA) indicated that the compounds such as CPT-11 of the formula 3 and 7-ethyl-10-hydroxycamptothecin (SN-38) of the formula 9 9 and Topotecan of the formula 4, in the presence of HSA at 37°C, exhibited a higher percentage (%) of lactone form at equilibrium than 20 (S) camptothecin of the formula 2 and 9-aminocamptothecin of the formula 5 [ T.G.Burke and Zihou Mi., J.Med. Chem . , 3_7 40 (1994); ibid.. Biochemistry. , 3_3_, 12540(1994)] . Based on these studies, it was recognized that the understanding of the factors influencing the lactone-carboxylate equilibrium of camptothecin analogues became an important determinant in the design of novel and therapeutically efficacious drug candidates in the camptothecin series. Although the modification of substituents on rings A and B of camptothecin was taken up at a rapid pace to generate novel CPT analogues, ring 'C analogues of camptothecins were limited presumably because of the research work carried out by Sawada et al. , which claimed that the substituents at C-5 position of camptothecin has resulted in the reduction of anti-tumor activity of camptothecins and produced inactive analogues [Sawada S.et.al., Chem. Pharm. Bull . , 39(10), 2574(1991)] . The C-5 substituted camptothecins claimed by Sawada et al. , (JP 58,154,584 ; US 4, 513,138 ; US 4, 473,692 ; US 4,545,880 ; US 4, 339, 282) have the structural formula 10, 10 where R represents hydroxy, lower alkyl, lower alkoxy or acyloxy; R represents hydrogen, methoxy at 9th position ; hydrogen, hydroxy, lower alkoxy, acyloxy, SH, thioalkyl, thioacyl, nitro, amino, alkylamino, 2 acylamino or halogen at 10th position and R represents hydrogen, lower alkyl, lower aralkyl, CH 2 OH, COOH, COOMe or CH 2 OR' where R' represents lower alkyl or acyl group. The recent findings by K.H.Lee et al. , [ Bio . Org. MedChem . Lett . , 5(1), 77(1995)] which includes the preparation of 5-hydroxymethyl camptothecin by the reaction of formaldehyde in N,N-dimethylformamide and 4-piperidinopiperidine on 20 (S) -camptothecin, has revealed the reduced anti-tumor activity of these compounds. Also, Danishefsky et al. , prepared some of the C-5 substituted 20 (RS) -camptothecin derivatives by a totally synthetic approach [US 5,391,745 and US 5,446,047] . However, the synthetically prepared 5- substituted camptothecin derivative of the formula 11 [Terasawa et. al. , Heterocycles, 3_8, 81 (1994) ] claimed to have anti-tumor activity comparable to that of 20 (S) -camptothecin. 11 Keeping all these factors in mind, we focused our research studies on 20 (S) -camptothecin aimed at the design of novel camptothecin analogues which can exhibit improved water solubility and improved stability of lactone form in solution. We identified an oxidative reaction in alcoholic solvents for this purpose. The resultant findings have culminated in the discovery of a novel synthetic transformation which can introduce a variety of alkoxy groups at C-5 position of 20 (S) -camptothecins of the formula 14, 14 where R through R have the meaning described above, the subject matter of which was described in our co¬ pending application for patent bearing Serial No. . Attorney Docket No. U 11024-5 There is provided a process for the preparation of the compounds of the formula 14, 14 wherein R , R , R , R are independently the same or different and represent hydrogen, hydroxy, aryloxy, lower alkoxy, lower alkanoyl, nitro, cyano, halo, carboxy, carbonyloxy, amino, substituted amino, lower alkyl, substituted lower alkyl or R 2 , R together represent -O-(CH 2 ) n -0- where n=l or 2; R 5 represents hydrogen, lower alkyl, substituted lower alkyl, lower aralkyl, hydroxyme hyl, carboxymethyl, aminomethyl, substituted aminomethyl where the amino group may be mono or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of a total of 5-6 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen or sulfur; and R 6 represents hydrogen; phenyl or benzyl where the phenyl group may be unsubstituted or substituted with mono, di or trisubstituents which may be selected from halogen, hydroxy, lower alkoxy, cyano, carboxyl, nitro, amino or substituted amino, lower alkyl, substituted lower alkyl; cycloalkyl or cycloalkyl lower alkyl where the cyclic ring is in the range of 3 membered to 7 membered ring system containing all carbon atoms; lower alkyl groups substituted with heterocyclic rings where the heterocyclic ring system has a total of 3 to 7 atoms, the ring system contains carbon with at least one heteroatom such as oxygen, nitrogen or sulfur; lower alkanoyl; benzoyl where the phenyl group can be unsubstituted or substituted; lower alkenyl; lower alkyl; substituted lower alkyl, substituted lower alkenyl or substituted lower alkanoyl where the substituents can be halogen, hydroxy, lower alkoxy, aryloxy, thio, thioalkyl, thioaryl, aryl or heteroaryl, carboxy, cyano, nitro, amido or amino in which the amino group can be unsubstituted or mono, or disubstituted in which both substituents are independent or combined together to form 5 or 6 membered cyclic ring system containing carbon, and optionally contains one or two heteroatoms selected from oxygen, nitrogen or sulfur, the total number of atoms in the cyclic ring system being 5 or 6; with the proviso that (i) when R is methoxy group, R is not hydrogen or lower alkyl group; (ii) when R 2 is hydroxy, lower alkoxy, thioalkyl, nitro, amino, alkylamino, acylamino, and halogen, R 6 is not hydrogen or lower alkyl group; (iii) when R 5 is lower alkyl, lower aralkyl, CH 2 OH, COOH, COOMe, or CH j OR" where R" represents lower alkyl or acyl group, R is not hydrogen or lower alkyl group (iv) when R is methoxy group, R is hydroxy, lower alkoxy, thioalkyl, nitro, amino, alkylamino, acylamino, or halogen, R is lower alkyl, lower aralkyl, CH 2 OH, COOH, COOMe or CH 2 OR" where R" represents lower alkyl or acyl group, R is not hydrogen or lower alkyl group; (v) when R through R represent hydrogen, R is not hydrogen or lower alkyl group, which comprises, (i) reacting the compounds of the formula 16, where R to R have the meaning described above, in the presence of an acid and an oxidizing agent which is a ferric salt, with a compound having the formula R -OH where R represents lower alkyl, lower alkenyl, (C 3 - C η ) cycloalkyl, haloalkyl or hydroxyalkyl, to obtain compounds of the formula 12 and compounds of the formula 17, ve, (ii) separating the compounds of the formulae 12 and 17 prepared in the step (i) , by conventional methods, (iii) hydrolyzing the compounds of the formula 17, by conventional methods, to obtain additional amounts of the compounds of the formula 12, (iv) reacting the compound of the formula 12, in the presence of an acid, with a compound having the formula R -OH to obtain compounds of the formula 14, where R , R 2 , R 3 , R 4 and R have the meaning described above and R is as defined above. Functional group transformation of these 5- alkoxy camptothecins of the formula 14 produced a wide variety of novel C-5 -C-substituted 20 (S) -camptothecins analogues of formula 1, which forms the subject matter of the present invention. Hence, the discovery led to a facile and versatile semi-synthetic methodology by which virtually every camptothecin derivative known in the literature can be transformed into a variety of C-5 substituted camptothecin analogues. In another co-pending application for patent bearing application serial no. , Attorney Docket No. U 011025-3 we have described and claimed the compounds of the formula 15, 15 where X represents NH or NR groups. Therefore, the present invention provides a novel process for the preparation of various C-5-C- substituted 20 (S) -camptothecin derivatives of the formula 1 where R has the meaning described above, starting from the compounds of the formula 12, 12 where R 1 through R 5 have the meaning described above. Furthermore, the vast variety of substituents on A, B and C rings represented by the compounds of formula 1 possess improved water solubility ranging from lmg to 5mg per ml. All of the compounds prepared by the present invention exhibited significant in vi tro anti- tumor activity against a wide range of human tumor cell lines. DETAILED DESCRIPTION OF THE INVENTION The present invention particularly provides C-5-C-substituted water soluble analogues of 20 (S) - Camptothecin having the formula 1, 1 wherein the groups R, R , R , R , R , R and R have the meaning described above. Throughout the present invention, the terms representing R, R - R in these compounds have the following definitions. The term 'lower alkyl' denotes a univalent, branched or straight hydrocarbon chain containing 1 to 8 carbon atoms. Representative examples of the alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, sec.butyl, tert.butyl, pentyl, isopentyl, tert. pentyl, hexyl, isohexyl and octyl. The term 'lower alkenyl' represents a branched or straight hydrocarbon chain having sp or sp 2 carbon centers containing 1 to 8 carbon atoms. Representative examples of the alkenyl groups are vinyl, propenyl, butenyl, pentenyl, isopropenyl, isobutenyl, proparginyl, hexenyl and oσtenyl. The term 'halogen' or 'halo' represents chlorine, bromine or fluorine. The term haloalkyl' denotes alkyl groups substituted with halogens, preferably fluorine, bromine or chlorine. Representative examples of the haloalkyl groups are chloroethyl, bromopropyl, fluoroethyl, trifluoroethyl, trichloroethyl and trifluorobutyl. The term ' lower alkoxy' denotes lower alkyl groups as defined above attached via oxygen linkage to the rest of the molecule. Representative examples of those groups are methoxy, ethoxy, isopropoxy, tert.butoxy, hexoxy, heptoxy and octoxy. The term 'lower alkanoyl' denotes lower alkyl or lower alkenyl groups as defined above attached via a carbonyl group to the rest of the molecule. Representative examples of those groups are acetyl, propionyl, propenσyl, crotanoyl, butanoyl, pentanoyl and isopentanoyl. The term 'aminoalkyl' represents the lower alkyl groups as defined above substituted with amino groups. Representative examples of the aminoalkyl groups are 2- aminopropyl, 4-aminobutyl and 5-aminopentyl. The amino groups may also be mono or disubstituted and representative examples of these substituted amino groups are dimethylamino, diethylaminσ, dibenzylamino, ethylisopropylamino, pyrrolidino, piperidino, morphilino and piperizino. The term 'heteroatom' refers to oxygen, nitrogen or sulfur. The term 'aryl' or 'heteroaryl' represents groups of aromatic nature having 5 or 6 membered rings which may be selected from phenyl, biphenyl, naphthyl, pyridyl, quinoline, isoquinoline, indole, pyroll, furan, benzofuran, thiophene, pyramidine, thiozolidine and imidazole. The term 'substituted phenyl' group used in the present invention refers to phenyl groups substituted with substituents which can be selected from the groups such as hydroxyl, lower alkyl, haloalkyl, phenyl, benzyl, halogen, lower alkoxy, thioalkoxy, benzyloxy, carboxyl, cyano, nitro, amido, amino, and alkylamino. Examples of such groups are 4-hydroxyphenyl, 3- methoxyphenyl, 4-fluorophenyl, 4-trifluoromethylphenyl, N,N-dimethylaminophenyl and 4-carbomethoxyphenyl. The term 'substituted alkyl' group used in the present invention refers to alkyl groups substituted with substituents which can be selected from the groups such as hydroxyl, alkyl, haloalkyl, phenyl, benzyl, halogen, alkoxy, thioalkoxy, benzyloxy, carboxyl, carbonyloxy, cyano, nitro, amido, amino and alkylamino. Examples of such groups are fluoroethyl, chloropropyl, hydroxyethyl, methoxypropyl, N,N-diethylaminoethyl, N- benzoylaminopropyl, trifluoroethoxyethyl, phenoxyethyl, carbo ethoxyethyl, (p-fluorobenzoyloxy) ethyl, a inopropyl and 2-thioethyl. The term 'substituted amino' group used in the present invention refers amino groups substituted with substituents which can be selected from the groups such as hydroxyl, alkyl, haloalkyl, benzyl, benzoyl, alkoxy, carboxyl, amido, amino, and alkylamino. Examples of such groups are N,N-diethylamino, N-benzoylamino, N- methoxyamino, N-carboethoxyamino, and N- cbloroethylamino groups. Also, both the substituents on the amino group can be combined together to form 5 or 6-membered cyclic ring system represented by pyrrolidino, piperidino, piperizino, morphilino, imidazolino, or thiazolidino. In our copending application Serial No. , Attorney Docket No. U 011024-5 we have described and claimed the compounds of the formula 12 and the process for their preparation, 12 where R 1 , R 2 , R 3 and R 4 are independently the same or different and represents hydrogen, hydroxy, lower alkoxy, aryloxy, lower alkanoyl, nitro, cyano, halo, carboxy, carbonyloxy, amino, substituted amino, lower alkyl, substituted lower alkyl or R 2 and R 3 combined together represent -0-(CH 2 ) n -0- where n=l or 2 ; R 5 represents hydrogen, lower alkyl, substituted lower alkyl, lower aralkyl, hydroxymethyl, carboxymethyl, aminomethyl or substituted aminomethyl where the amino group is mono or disubstituted in which both substituents are independent or combined together to form 5 or 6 membered cyclic ring system containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur. Employing the compounds of the formula 12, we have prepared the compounds of the formula 1 as described in the present invention. Accordingly the present invention also provides a process for the preparation of the compounds of the general formula 1, wherein R 1 , R 2 , R 3 and R are independently the same or different and represent hydrogen, hydroxy, lower alkoxy, aryloxy, lower alkanoyl, nitro, cyano, halo, carboxy, carbonyloxy, amino, substituted amino, lower alkyl, substituted lower alkyl or R 2 and R3 combimnied together represent -0- (CH- 2 ) n -0- where n=l or 2; R.5" represents hydrogen, lower alkyl, substituted lower alkyl, lower aralkyl, hydroxymethyl, carboxymethyl, aminomethyl, substituted aminomethyl where the amino group is mono or disubstituted in which both substituents are independent or combined together to form 5 or 6 membered cyclic ring system containing carbon, and optionally contains one or two heteroatoms selected from oxygen, nitrogen and sulfur. The number of atoms in the cyclic ring system is 5 or 6. When R represents hydrogen, R represents lower alkoxy; carbonyloxy; cyano; nitro; thio; thioalkyl; thioaryl; amide or amino group in which the amino group can be unsubstituted or mono or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of 3 to 8 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur; phenoxy, phenyl, benzoyl or benzyl where the phenyl group can be unsubstituted or substituted with mono, di or trisubstituents which may be selected from halogen, hydroxy, alkoxy, cyano, carboxy, nitro, amido, amino or substituted amino, thioalkoxy, phenyl, benzyl, lower alkyl or substituted lower alkyl; cycloalkyl or cycloalkyl lower alkyl where the cyclic ring may be in the range of 3 membered to 7 membered ring system containing all carbon atoms; lower alkyl groups substituted with heterocyclic rings where the heterocyclic ring system having 3 to 7 atoms contains carbons with at least one heteroatom selected from oxygen, nitrogen and sulfur; lower alkanoyl; lower alkenyl; substituted lower alkyl or substituted lower alkenyl where the substituents can be halogen, hydroxy, benzoxy, carbonyloxy, alkoxy, aryloxy, carboxyl, cyano, thio, thioalkyl, thioaryl, aryl, heteroaryl, nitro, amido or amino in which amino group can be unsubstituted or mono, or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of 3 to 8 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur, or R 6 represents COOR' where R' represents hydrogen, lower alkyl, substituted lower alkyl or lower aralkyl; when R represents lower alkyl; lower alkenyl; lower alkanoyl; substituted lower alkyl; substituted lower alkanoyl; phenyl, benzyl or benzoyl in which the phenyl group may be unsubstituted or substituted; or lower alkoxycarbonyl; R represents phenyl; benzoyl; cyano; nitro; lower alkanoyl or COOR' group in which R' represents hydrogen, lower alkyl, lower aralkyl or substituted lower alkyl which comprises, i) reacting the compounds of the formula 12, 12 where R through R have the meaning described above 1, with a compound of the formula R -CH 2 -J where R represents lower alkyl, substituted lower alkyl, cycloalkyl, cycloalkyl lower alkyl, lower alkanoyl, lower alkenyl, cyano, nitro, thio, thioalkyl, thioaryl, phenoxy, phenyl, benzyl or benzoyl or COOR' group in which R' denotes lower alkyl or aralkyl group and J represents halogen, trialkylsilyl, trialkyltin, triphenylphosphonium salt or its derivative, trialkyl phosphonium salt or its derivative or J may represent MgG where G denotes halogen other than fluorine, in the presence of suitable acid or base or a metal such as lithium or zinc, as per the reaction conditions, to obtain compounds of the formula 13, 13 where R 1 , R 2 , R 3 , R 4 , R 5 and R 6 have the meaning given above, (ii) reacting the compounds of the formula 13 where R through R have the meaning described above and R represents COOR' where R' denotes lower alkyl groups with an inorganic base followed by acidification to obtain the compounds of the formula 13, 13 where R 1 through R 5 have the meaning described above and R 6 denotes COOH group; (iii) reacting the compounds of the formula 13, where R through R have the meaning described above and R 6 denotes COOH group, with compounds of the formula R' -OH where R' represents lower alkyl, aralkyl, haloalkyl, hydroxyalkyl, alkoxyalkyl or aminoalkyl, in the presence of an acid to obtain the compounds of the formula 13, 13 where R 1 through R 5 have the meaning described above and R denotes COOR' group in which R' represents lower alkyl, aralkyl, haloalkyl, hydroxyalkyl, alkoxyalkyl or aminoalkyl; (iv) reacting the compounds of the formula 13, where R through R 5 have the meaning described above and R 6 denotes COOH group, with amines having the formula NHR 7 R 8 , where R 7 and R 8 are independently the same or different and represent hydrogen, lower alkyl, haloalkyl, hydroxyalkyl or aminoalkyl or R 7 and R 8 are combined together to form a 5 to 8 membered cyclic ring system containing carbon, and at least one heteroatom selected from oxygen, sulfur or nitrogen to obtain the corresponding amides of the formula 13, 13 where R 1 through R have the meaning described above and R 6 denotes CONR 7 R 8 group where R 7 or R 8 are independently the same or different and represent hydrogen, lower alkyl, haloalkyl, hydroxyalkyl or aminoalkyl or R 7 and Rfi combined together to form a 5 to 8 membered cyclic ring system containing carbon and at least one heteroatom selected from oxygen, sulfur or nitrogen; (v) reacting the compounds of the formula 13 where R 6 represents phenyl, benzoyl, cyano, nitro, lower alkanoyl or COOR' group in which R' denotes hydrogen, substituted lower alkyl, lower alkyl, or aralkyl, or a group NR 7 R 8 where R 7 and R 8 have the meaning described in step (iv) , in the presence of a base, with a reagent having the formula R-G where R is not hydrogen and G represents halogen to obtain compounds of the formula 1, 1 where R represents lower alkyl, substituted lower alkyl, lower alkenyl, lower alkanoyl, substituted lower alkanoyl, phenyl, benzyl or benzoyl in which the phenyl group may be unsubstituted or substituted and R through R have the meaning described above Accordingly, for the first time a process for the preparation of C-5 substituted camptothecin derivatives of the formula 1, starting from the compounds of formula 12 using a semi -synthetic approach has been developed. The compounds of the formula 1 prepared by the process of the present invention thus represents diastereomers containing the newly created C-5 chiral center. Indeed, the compounds of the general formula 1 are isolated as a mixture of 20(S),5(R) and 20 (S) ,5(S) diastereomers. However, by the application of conventional analytical techniques, the two diastereomers have also been separated into their single optically pure entities. In general, compounds of the formula 1 where R, R 1 , R 2 , R 3 , R 4 , R 5 and R 6 have the meaning described above, may be synthesized starting from the compounds of the formula 12 following the process described in the present invention and can be illustrated with the examples described in the Examples Section. According to the process of the present invention, C-5 substituted carbon derivatives having the formula 13 can be prepared by reacting the compounds of the formula 12 where R 1 to R 5 have the meaning given above, with a compound of the formula R 6 - CH 2 -J where R 6 represents lower alkyl, substituted lower alkyl, cycloalkyl, cycloalkyl lower alkyl, lower alkanoyl, lower alkenyl, cyano, nitro, thio, thioalkyl, thioaryl, phenoxy, phenyl, benzyl, benzoyl or COOR' group in which R' represents lower alkyl or lower aralkyl groups and J represents halogen, trialkylsilyl, trialkyltin, triphenylphosphonium salt or its derivative, trialkylphosphonium salt or its derivative or J can also represent Mg-G where G denotes halogen other than fluorine, in the presence of suitable acid or base or a metal such a lithium, sodium or zinc as per the reaction conditions. The acid used in the reaction may be selected from mineral acids such as hydrochloric acid, sulfuric acid or Lewis acids such as BF 3 -etherate, titanium tetrachloride, tin chloride, zinc chloride or stannous chloride. The base used in the reaction may be selected from sodium hydride, potassium hydride, sodium carbonate, potassium carbonate, pyridine, triβthylamine, N,N- diisopropylethylamine, N-N-dimethylaminopyridine, dicyclohexyl-carbodiimide, hexamethyldisilazane, lithium bases such as n-butyl lithium, sec.butyl lithium, lithiumhexamethyldisilazide, lithium diisopropylamide or sodium methoxide, potassium tert. butoxide, or sodium tert.amyloxide. The solvent used in the reaction may be selected from hexane, benzene, toluene, ether, tetrahydrofuran, dimethoxyethane, dichloromethane and chloroform. The reaction can be performed at a temperature in the range of -100°C to +100°C, preferably in the range of -70°C to +80°C. In the step(ii), the compounds of the formula 13 where R 1 through R have the meaning described above and R represents COOR' where R' denotes lower alkyl groups were reacted with an organic base followed by an acid work up to obtain the compounds of the formula 13, where R through have the meaning described above and R denotes COOH group. The inorganic base used in the reaction may be selected from sodium hydroxide, potassium hydroxide, lithium hydroxide or carbonates such as potassium carbonate, sodium carbonate, or lithium carbonate dissolved in aqueous medium. The reaction can be performed at a temperature in the range of 20°C to 100°C. To obtain the compounds of the formula 13, where R 6 represents COOR' in which R' denotes lower alkyl, arylalkyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, the compounds of the formula 13 where R through R have the meaning described above and R represents COOH group are reacted with compounds of the formula R' -OH where R' represents lower alkyl, arylalkyl, haloalkyl, hydroxyalkyl, alkoxyalkyl or aminoalkyl, in the presence of an acid as step (iii) of the present invention. The acid used in the reaction may be selected from mineral acids such as hydrochloric acid, sulfuric acid or Lewis acids such as BF 3 - etherate, titanium tetrachloride, tinchloride, zinc chloride or stannous chloride. The solvent used in the reaction may be selected from benzene, toluene, tetrahydrofuran, dimethoxyethane, dioxane, dichloroethane, dichloromethane or chloroform. The reaction can be performed at a temperature in the range of 0°C to 100°C, preferably in the range of 25°C to 80°C. Similarly in step (iv) of the process of the present invention, to obtain the compounds of the formula 13, where R represents amide groups such as CONR 7Rβ where R7 or R8 are independently the same or different and represent hydrogen, lower alkyl, haloalkyl, hydroxyalkyl or aminoalkyl or R 7 and R 8 combined together form a 5 or 6 membered cyclic ring system containing carbon and at least one oxygen or nitrogen atom, the compounds of the formula 13 were reacted with primary or secondary amines. The amines used in the present invention may be selected from methylamine, ethylamine, diethylamine, dimethylamine, pyrrolidine, piperidine, morpholine, piperizine, imadazole and pyrazine. The solvent used in the reaction may be selected from benzene, toluene, tetrahydrofuran, dimethoxyethane, dioxane, dichloroethane, dichloromethane or chloroform. The temperature used in the reaction may be in the range of 20°C to 100°C, preferably in the range of 25°C to 80°C. Finally, the compounds of the formula 1 can be prepared as stated in the step(v) of the process of the present invention by reacting the compounds of the formula 13 where R represents groups such as phenyl, benzoyl, cyano, nitro, lower alkanoyl or COOR' where R' denotes hydrogen, lower alkyl, substituted lower alkyl or aralkyl, in the presence of a base, with reagents having the formula R-G where G represents halogen and R has the meaning other than hydrogen as described above. The base employed in the reaction may be organic bases such as sodium hydride, potassium hydride, lithium bases such as n-butyl lithium, sec.butyl lithium, lithiumhexamethyldisilazide, lithium diisopropylamide or alkoxides such as sodium methoxide or potassium tert.butoxide. The solvent used in the reaction can be selected from diethylether, tetrahydrofuran, dime hoxyethane, benzene, toluene, methanol, ethanol or tert.butanol. The reaction can be effected at temperatures ranging from -78°C to +80°C. Thus, the present invention is of particular significance in developing 5-C-substituted 20 (S) - camptothecin derivatives as a new class of C-ring modified camptothecin analogues which are useful as anti-tumor and/or anti-viral agents. The present invention is also of particular significance as the process developed and described here is highly versatile and amenable for large scale preparation of these camptothecin derivatives having the general formula 1. The methodology developed and described in the present invention will provide access to a wide variety of C-5-Carbon substituted C-ring analogues having diverse substituents on rings A and B of 20 (S) - camptothecin. Some of the preferred compounds are those where R is nitro, amino, aminoalkyl, hydroxy or methoxy; R 2 is hydroxy, carbonyloxy or halo; R 2 and R 3 combined together represent methylenedioxy or ethylenedioxy; R is nitro; R is ethyl, aminomethyl or piperizinomethy1; R is carboxyl, carbomethoxy, carboethoxy, CHO group, cyano, or COOR' where R' is hydroxyethyl; trifluoroethyl; fluoroethyl; aminoethyl, substituted aminoethyl or acetamido where the amino group is unsubstituted pyrrolidino, piperidino, morphilino, diethylamino or diisopropylamino. Representative compounds of formula 1 are: 1) 5-Carbomethoxymethyl CPT 2) 5- (2' -Oxopropyl) CPT 3) 5-Carboethoxymethyl CPT 4) 5-Carboxymethyl CPT 5) 9-Nitro-5-carbomethoxymethyl CPT 6) 9-Nitro-5- (2Oxopropyl) CPT 7) 9-Nitro-5-carboxymethyl CPT 8) 10-Hydroxy-5-carbomethoxymethyl CPT 9) 10-Hydroxy-5- (2' -oxopropyl) CPT 10) 10-Hydroxy-7-ethyl-5- (2' -oxopropyl) CPT 11) 10-Hydroxy-9- (N,N-dimethylaminomethyl) -5- carboxymethyl CPT 12) 5-Acetamido CPT 13) 5- (2' -Oxopyrrolidinoethyl) CPT 14) 5- (2' -Oxopiperidinoethyl) CPT 15) 5-Carboxymethyl camptothecin, fluoroethyl ester 16) 5-Carboxymethyl camptothecin, hydroxyethyl ester 17) 5-Carboxymethyl camptothecin, trifluoroethyl ester 18) 9-Methoxy-5-carboethoxymethyl CPT 19) 9-Hydroxy-5-carboethoxymethyl CPT 20) 9-Methoxy-5-carboxymethyl CPT 21) 5- (2' -Hydroxyethyl) CPT 22) 12-nitro-5-carbomethoxy methyl CPT 23) 12-nitro-5- (2' -oxopropyl) CPT where CPT refers to 20 (S) -camptothecin In fact, most of the compounds prepared by the present invention have water solubility ranging from 1 mg to 5mg per ml at 37 C. Further, several compounds prepared in the present invention exhibited good in vi tro anti-cancer activity towards various human tumor cell lines, according to the results obtained from 60 human tumor cell line assay performed at National Cancer Institute (NCI) , Bethesda, Maryland, U.S.A. The results shown in Tables 1 and 2 were obtained from conducting experiments according to U. S. National Cancer Institute (NCI) protocols as given below: Each test compound was screened against a battery of 60 human cell lines obtained from eight organs. In a typical procedure, the cell suspensions that were diluted according to the particular cell type and the expected target cell density (5000-40,000 cells per well based on cell growth characteristics) were added into 96-well microtiter plates. Inoculates were allowed a preincubation period of 24h at 37 C for stabilization. Dilutions at twice the intended test concentrations were added at time zero in 100-μl aliquots to microtiter plate wells. Usually test compounds were evaluated at five 10-fold dilutions. The highest well concentration used in the test is 10 M. The cells are then incubated in the presence of drug (the test compound) for further 48h in 5% C0 2 atmosphere and 100% humidity. At the end of this time, the adherent cells are fixed to the plate by means of trichloroacetic acid, and after a number of washes, the cell layer is treated with the protein stain Sulforhodamine B. The optical density which is proportional to protein mass, is then read by automated spectrophotometric plate readers at a wavelength of 515 nm. Readings are transferred to a microcomputer and final reports are generated using especially developed software. TABLE 1: S. NO. COMPOUND IC 50 (μm) 1) 5-Carbomethocymethyl CPT 16 .2 2) 5- (2' -Oxopropyl) CPT 8 .31 3) 10-Hydroxy-5-carbomethoxymethyl CPT 4 . 89 4) 9-Nitro-5- (2' -oxopropyl) CPT 1 . 00 5) 9-Nitro-5-carbomethoxymethyl CPT 15 . 8 6) 5-Ace amido CPT 26 . 9 7) 9-Methoxy-5-carbomethoxy CPT 16 .2 IC 50 = the mean value of the minimum drug concentration (μm) of the agent required to produce 50% cell growth inhibition (GI50) against NCI's 60 human tumor cell line assay. TABLE 2 : In Vitro ANTI-CANCER activity data for EXAMPLES 2 AND 4 CELL PANEL CELL LINE EXAMPLE 2 EXAMPLE 4 LEUKEMIA CCRF-CEM 0.38 0.09 MOLT-4 0.77 HL 60 0.23 SR 0.18 0.02 NSLC H 460 1.12 0.10 HOP 62 4.46 0.26 H 522 3.98 H 23 0.24 COLON HCT 116 5.62 0.57 SW 620 6.02 0.07 HT 29 1.17 CNS SF-268 4.26 SF-539 3.89 0.26 SNB-19 6.45 SF 295 0.19 RENAL 786-0 2.95 0.30 ACHN 2.88 0.21 CAKI-1 2.63 0.21 CONTINUATION OF TABLE 2 CELL PANEL CELL LINE EXAMPLE 2 EXAMPLE 4 MELANOMA LOX IMVI 0.38 M 14 • 0.30 UACC 62 0.33 BREAST MCF-7 3.63 MCF7/ADR 1.86 T 47D 2.63 The data shown here refers to 50% Growth Inhibition (GI 50) values in μm concentrations. All these compounds of the general formula 1 of the present invention, including the pharma¬ ceutically acceptable salts thereof, and the composition containing them, are useful as anti-cancer and anti-viral agents. Administration of the active compositions of the formula 1, in pure form or in an appropriate pharmaceutical composition can be carried out via any of the accepted modes of administration for serving similar utilities. Thus, administration can be, for example, orally, nasally, parenterally or topically, in the form of solid, semi-solid, lyophilized powder, or liquid dosage forms, such as for example, tablets, suppositories, pills, capsules, powders, solutions, suspensions, emulsions, creams, lotions, aerosols, ointments, injections or the like, preferably, in unit dosage forms suitable, for simple administration of precise dosages. The compositions will include a conventional pharmaceutical carrier or excipient and an active compound of general formula 1 and, in addition, may include either medicinal agents, pharmaceutical agents, carriers, adjuvants, etc. The invention is described in detail with specific examples given below which are provided by way of illustration only and should not be constructed to limit the scope of the invention. EXAMPLES: EXAMPLE 1 Preparation of 5-Carbomethoxymethylcamptothecin To a solution of lOO g of 5-hydroxycamptothecin of the formula 12 where R 1 «R 2 =R 3 =R 4 =R 5 sH, dissolved in 12ml of benzene and 0.1ml of dry pyridine, in 5ml of benzene was added 0mg of carbomethoxymethylene triphenyl- o phosphorane and stirred at 80 C for 16h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 65mg of 5-carbomethoxymethyl camptothecin of the formula 1; mp 235°C; IR: (KBr) : 3444, 2925, 1746 (br), 1660, 1607, 1150, 721 cm "1 ; ^ NMR (CDC1 3 , 200MHz) : δ 8.41(s, IH) , 8.23(d, J=8.4Hz, IH) , 7.92(d, J=8Hz, IH) , 7.82 (t, J= 7.4Hz, IH) , 7.71-7.58 (m,2H) , 6.06(d, J=8Hz,0.5H), 6.03 (d, J=8Hz,0.5H), 5.71(d, J=16hz IH) , 5.28(d, J=16Hz, IH) , 3.92 (dd, J=17Hz,3.4, IH) , 3.70(s, 3H) , 3.35(dd, J=17Hz, 7.6Hz, IH) , 2.05- 1.85 (m, 2H) , 1.05 (t, 8Hz, 3H) , Mass (m/z) : 420 (M+1), 376, 361, 347, 317, 277, 201, 152, 95, 85 EXAMPLE 2 Preparation of 5- (2' -Oxopropyl) camptothecin To a solution of 200mg of 5-hydroxycamptothecin of the formula 12 where R 1 =R 2 =R 3 =R 4 =R 5 =H, dissolved in 16ml of toluene and 0.2ml of dry pyridine, 140mg of 1- triphenylphosphoranylidene-2-propanone in 8 ml of o toluene was added and stirred at 100 C for 16h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 140mg of 5- (2' -oxopropyl) camptothecin of the formula 1; IR: (KBr): 3424, 1745, 1715, 1659, 1606, 1155 cm "1 ; X H NMR (CDC1 3 200MHz ) : δ 8.40 (s, IH) , 8.23 (d, J=8.4Hz, IH) , 7.90 (d, J=8Hz, IH) , 7.81 ( t, J=7.4Hz, IH) , 7.71-7.58(m,2H) , 6.11(d, J=7.2Hz, 0.5H) , 6.06(d, J=7.2Hz,0.5H) , 5.68(d, J=16Hz, IH) , 5.26 (d, J=16Hz, IH) , 4.27(dd, J=16.2Hz,3.4Hz, IH) , 3.75(s, IH, D 2 0 exchangeable), 2.81(dd, J=17Hz, 7.6Hz,0.5H), 2.71(dd, J=17Hz, 7.6Hz,0.5H), 2.24(s, 3H) 2.01- 1.81(m,2H) , 1.05 (t, 8Hz, 3H) ; Mass (m/z) : 404 (M+1), 391, 361, 347, 317, 231, 128, 107, 89. EXAMPLE 3 Preparation of 10-Hydroxy-5 carbomethoxymethyl camptothecin To a solution of lOOmg of 10,5-dihydroxycamptothecin of the formula 12 where R 2 =OH, R 1 =R 3 =R 4 =R 5 =H, dissolved in 20ml of benzene was added 0.1ml of dry pyridine and 60mg of carbomethoxymethylene triphenylphosphorane. o The resulting solution was stirred at 80 C mg for 16h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 65mg of 10-hydroxy-5-carbomethoxymethyl- camptothecin of the formula 1; mp : 153°C : 1 H NMR (CDC13 + DMS0-d6), δ 8.18 (s, IH) , 8.02 (d, J=9Hz, IH) , 7.58(s, IH) , 7.46(d, J=9Hz, IH) , 7.18(s,lH), 5.95(dd, J=7.2Hz, 3.2Hz, 0.5H), 5.90 (dd, J=7.2Hz, 3.2Hz, 0.5H) , 5.65( d, J=16Hz, IH) , 5.25 (d, J=16Hz, IH) , 3.84 ( dd, J=17Hz, 3.4Hz, IH) , 3.69(s, 3H) , 3.05 (br s, IH, D 2 0 exchangeable), 2.91(dd, J=17Hz, 7.6Hz, IH) , 1.95(m,2H), 1.05 (t, J=7Hz, 3H) ; Mass (m/z) : 436 (M+1), 407, 392, 361, 347, 333, 247, 169, 107. EXAMPLE 4 Preparation of 9-Nitro-5- (2' -oxopropyl)camptothecin To a solution of lOOmg of 9-nitro-5-hydroxycamptothecin of the formula 12 where R 1 =N0 2 ,R 2 =R 3 =R 4 =R 5 =H, dissolved in 10ml of toluene wand 0.3 ml of dry pyridine, 80mg of l-triphenylphosphoranylidene-2-propanone in 5ml of toluene was added and stirred at 100°C for 16h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 80mg of 9-nitro-5- (2' -oxopropyl)camptothecin of the formula 1; mp : 137°C : IR : 3384, 1746, 1660, 1603, 1526, 1154 cm "1 ; 1 H NMR (CDC1 3 ) : δ 9.21 (s, IH) , 8.53 (d, J=8.6Hz, IH) , 8.46(d, J=8.6Hz,lH), 7.91(t, J=8Hz, IH) , 7.68(s,0.5H) , 7.66(s, 0.5H) , 6.04(dd, J=7.2Hz, 3Hz, 0.5H), 6.06 (dd, J=7.2Hz, 3Hz, 0.5H) , 5.75( d, J=16Hz, 0.5H), 5.73( d, J=16Hz, 0.5H) , 5.37( d, J=16Hz, 0.5H), 5.24(d, J=16Hz, 0.5H), 4.18 (dd, J=17Hz, 3.4Hz, 0.5H), 4.16 (dd, J=17Hz, 3.4Hz, 0.5H) , 3.85 (br s, IH, D 2 0 exchangeable), 3.35 (dd, J=17Hz, 7.6Hz, 0.5H), 3.25 (dd, J=17Hz, 7.6Hz, 0.5H), 2.26(s, 3H) , 1.96(m,2H), 1.12 (t, J=7Hz, 3H) ; Mass (m/z) : 450(M+1), 419, 406, 376, 362, 332, 319, 304, 232, 159, 101. EXAMPLE 5 Preparation of 9-Nitro-5-carbomethoxymethylcamptothecin To a solution of 150mg of 9-nitro-5-hydroxycamptothecin of the formula 12 where R 1 =N0 2 ,R 2 =R 3 =R 4 =R 5 =H, dissolved in 20ml of benzene was added 0.4 ml of dry pyridine, 80mg of carbomethoxymethylene triphenylphosphorane. The resulting solution was refluxed for 24h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 110 mg of 9-nitro-5-carbomethoxymethyl camptothecin of the formula 1; mp : 140°C : IR : 3320, 1740, 1661, 1604, 1526, 1154, 1050, 740 cm "1 ; X H NMR (CDC1 . # δ 9.21 (s, IH) , 8.53 (d, J=8.6Hz, IH) , 8.46(d, J=8.6Hz,lH), 7.91(t, J=8Hz, IH) , 7.68 (s,0.5H) , 7.66(s, 0.5H), 6.19-6.03 (m,IH) , 5.75 (d, J=16Hz, 0.5H), 5.73( d, J=16Hz, 0.5H) , 5.37{ d, J=16Hz, 0.5H) , 5.24( d, J=16Hz, 0.5H), 3.82(d, J=17Hz, 0.5H), 3.80(d, J=17Hz, 0.5H), 3.85 (br, s, IH, D 2 0 exchangeable), 3.68(s, 3H) , 3.35(dd, J=17Hz, 7.6Hz, 0.5H), 3.25(dd, J=17Hz, 7.6Hz, 0.5H), 1.96(m,2H), 1.02 (t, J=7Hz, 3H) ; Mass (m/z) : 465(M+1), 435, 422, 406, 391, 362, 332, 277, 169, 91. EXAMPLE 6 Preparation of 5-acetamidocamptothecin Step 1: 5-Carbomethoxymethylcamptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOMe was prepared as described in the example 1. Step 2: lOOmg of 5-carbomethoxymethylcamptothecin of the formula 1 where was dissolved in 10ml of 40% aqueous diethylamine and the contents were heated to reflux for lOh. The reaction mixture was neutralized with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water and brine. Evaporation of the solvent gave an oily residue which was purified over silica gel column chromatography using chloroform-acetone solvent mixture as eluent to obtain 65mg of 5-acetamidocamptothecin of the formula 1, R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =C0NH 2 ; mp : 250°C : IR : 3230., 2927, 1746, 1658, 1584, 1402, 1152, 1050, 785, 541 cm "1 ; Partial X H NMR data in (CDCl 3 + DMS0-d6) : δ 6.05(d, J=8Hz,0.5H) 5.95 (d, J=8Hz, 0.5H) , 5.62 (d, J=16Hz,lH), 5.26 (d, J=16Hz, IH) , 3.91 (dd, J=17Hz,3.4Hz, IH) , 3.70(s, 3H) , 3.31(br s, D 2 0 exchangeable,IH) , 3.35(dd, J=17Hz,7.6Hz,IH) , 2.00- 1.84(m,2H), 1.07 (t, J=8Hz, 3H) ; Mass (m/z) : 406 (M+1) , 362, 347, 333, 317, 305, 289, 262, 205, 159, 107, 84. EXAMPLE 7 Preparation of 5 - (2 Oxopyrrolidinoethyl) camptothecin Step 1 : 5 -Carbomethoxymethylcamptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOMe was prepared as described in the example 1. Step 2: lOOmg of 5-Carbomethoxymethylcamptothecin of the formula 1 where R=R=R 2 =R 3 =R 4 =R 5 =H and R 6 =COOMe was dissolved in 10ml of pyrolidine and heated to reflux for 16h. Excess pyrolidine was removed under vacuum and the residue was stirred with 20ml of 10% HCl for 30min. Then, the aqueous solution was extracted with ethyl acetate and the organic layer was washed with brine and concentrated. The gummy solid was purified over column chromatography using chloroform-acetone solvent mixture to get 65mg of 5- [2' -oxopyrrolidino- ethyl] camptothecin of the formula 1, R=R 1 sR 2 =R 3 =R 4 =R 5 -=H, R 6 =CON(CH 2 ) 4 ; mp : IR : 3387, 1747, 1659, 1615, 1446, 1408, 1141, 621 cm "1 : Partial ΕL NMR data in CDC1 3 δ 6.22(d, J=8Hz,0.5H) 6.15(d, J=8Hz, 0.5H) , 5.72 (d, J=16Hz,lH), 5.26(d, J=16Hz, IH) , 4.15(d, J=16Hz, IH) , 3.81 (br s, D 2 0 exchangeable, IH) , 3.71-3.18 (m,4H) , 2.51-2.29(m,lH) , 2.15-1.85 (m, 6H) , 1.18-1.02 ( , 3H) ; Mass(m/z) : 460 (M+1) , 416, 388, 360, 347, 316, 259, 231, 191, 128, 98, 83. EXAMPLE 8 Preparation of 5- (2' -Oxopjperidinoethyl) camptothecin Step 1: 5-Carbomethoxymethylcamptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOMe was prepared as described in the example 1. Step 2: 125mg of 5-Carbomethoxycamptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOMe,was dissolved in 12ml of piperine and heated to reflux for 16h. Excess piperidine was removed under vacuum and the residue was stirred with 20ml of 10% HCl for 30min. Then, the aqueous solution was extracted with ethyl acetate and the organic layer was washed with brine and concentrated. The resultant residue was purified over column chromatography using chloroform-acetone solvent mixture to get 75mg of 5- [2' -oxopiperidinoethyl) camptothecin of the formula 1, R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =CON(CH 2 ) 5 ; mp: 246°C IR : 3385, 1749, 1649, 1614, 1450, 1404, 1228, 1154, 1050, 759, 559, 549 cm "1 : Partial K NMR in CDC1 3 . δ 6.25 (d, J=8Hz, 0.5H) 6.15(d, J=8Hz,0.5H), 5.75(d, J=16Hz,lH), 5.29(d, J=16Hz, IH) , 4.35-4.17 (m, IH) , 3.81(br d, D 2 0 exchangeable, IH) , 3.85-3.21 ( ,4H) , 2.54-2.34 (m,IH) , 2.05-1.85(m,6H) , 1.75-1.38 (m,6H) , 1.18-1.02 (m,3H) ; Mass(m/z) : 474 (M+1) , 430, 388, 360, 344, 316, 287, 259, 231, 190, 126, 86. EXAMPLE 9 Preparation of 10-Hydroxy-5- (2'oxopropyl) camptothecin To a solution of lOOmg of 10,5-dihydroxycamptothecin of the formula 12 where R 2 =OH,R 1 =R 3 =R 4 =R 5 =H, dissolved in 10ml of toluene was added 0.3 ml of dry triethylamine, 80mg of 1-triphenylphosphoranylidene-2-propanone in 5ml of toluene was added and stirred at 100°C for 24h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 60 mg of 10-hydroxy-5- (2Oxopropyl) camptothecin of the formula 1; mp : 170°C : H NMR (CDCl 3 +DMSO-d6) , : δ 8.18 (s, IH) , 8.02 (d, J=9Hz, IH) , 7.58(s, IH) , 7.46(d, J=9Hz, IH) , 7.18(s, IH) , 5.95 (dd, J=7.2Hz,3.2Hz, 0.5H), 5.90 (dd, J=7.2Hz, 3.2Hz, 0.5H) , 5.65 (d, J=16Hz, IH) , 5.25 (d, J=16Hz, IH) , 4.18( dd, J=17Hz, 3.4Hz, IH) , 3.05 (br s, lh, D 2 0 exchangeable), 2.91-2.78(m, IH) , 2.26(s,3H), 1.95(m,2H), 1.05(t, J=7Hz, 3H) ; Mass (m/z) : 408 (M+1), 391, 375, 361, 333, 319, 279, 263, 175. EXAMPLE 10 Preparation of 9-methoxy-5-carbomethoxymethyl camptothecin A solution of 150mg of 9-Methoxy-5-hydroxycamptothecin of the formula 12 where R 1 =OMe,R 2 =R 3 =R 4 =R 5 =H, dissolved in 12ml of tetrahydrofuran was added to 1.5equiv., of sodium hydride in 5ml of tetrahydrofuran followed by the addition of 60mg of carbomethoxymethylene triphenylphosphorane and stirred at 25°C for 16h. At the end, reaction mixture was acidified with 5% HCl and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over anh.sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column chromatography furnished 85mg of 9-methoxy-5-carbomethoxymethyl camptothecin of the formula 1. EXAMPLE 11 Preparation of 5-Carboxymethyl camptothecin Step 1: 5-Carbomethoxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOMe was prepared as described in the example 1. Step 2: To a methanolic solution of lOOmg of 5- carbomethoxy camptothecin was added 2ml of 0.IN sodium hydroxide solution and stirred at 25°C for lOh. Reaction mixture was acidified with dilute HCl and the aqueous layer extracted with 10% methanol- chloroform solvent mixture. Organic layer was washed with brine solution and the solvent concentrated to a minimum volume. The resulting residue was purified over silica gel column chromatography to get 75mg of 5-carboxy¬ methyl camptothecin of the formula 1, R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =C00H. : p : 240°C ; IR : 2973, 1755, 1655, 1579, 1560, 1154 cm "1 : H NMR in CDC1 3 + DMSO-d6, 200 MHz) : δ 8.6.0(s, IH) 8.23(d, J=8Hz, IH) , 8.15(d, J=8Hz, IH) , 7.85(t, J=7.4Hz, IH) , 7.68(t, J=7Hz,lH), 7.50(s,lH), 6.40 (s,lh, D 2 0 exchangeable), 6.06- 5.85 (m, IH) , 5.55 (d, J=16Hz, IH) , 5.28 (d, J=16Hz, IH) , 4.15-3.92 (dd, J=17Hz,3.4Hz, IH) , 3.35(dd, J=17Hz, 7.6Hz, IH) , 2.05-1.90(m,2H) , 1.05(t, 8Hz, 3H) ; 13 C NMR (50MHz, DMSO) : 172.5, 170.9, 157.2, 152.2, 150.2, 150.0 148.1, 145.6, 133.2, 130.7, 130.4, 129.0, 128.6, 127.9, 127.6, 119.8, 96.8, 83.6, 72.4, 65.3, 59.0, 34.3, 33.8, 30.3, 7.8; Mass(m/z) : 406(M+1), 378, 362, 348, 319, 277, 191, 111. EXAMPLE 12 Preparation of 10-hydroxy-5-carboxymethyl camptothecin Step 1: lO-Hydroxy-5-carbomethoxymethyl camptothecin of the formula 1 where R 2 =OH,R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOMe was prepared as described in the example 3. Step 2: To a methanolic solution of 150mg of 10- hydroxy-5-carbomethoxymethyl camptothecin was added 3ml of 0.1N sodium hydroxide solution and stirred at 25°C for lOh. Reaction mixture was acidified with dilute HCl and extracted the aqueous layer with 10% methanol- chloroform solvent mixture. Organic layer was washed with brine solution and concentrated the solvent to a minimum volume. The resulting residue was purified over silica gel column chromatography to get 105mg of 10-hydroxy-5-carboxymethyl camptothecin of the formula 1, R 2 =OH,R=R 1 =R 3 =R 4 =R 5 =H and R 6 =COOH. EXAMPLE 13 Preparation of 5-Carboxymethyl camptothecin, fluoroethyl ester Step 1: 5-Carboxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOH was prepared as described in the example 11. Step 2: lOOmg of 5-carboxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOH, dissolved in 15ml of dichloroethane was treated with 0.5ml of 2- fluoroethanol and the contents heated to 80°C for lOh in the presence of cat. amount of sulfuric acid. Reaction mixture was neutralized with pyridine and diluted with ethyl acetate. Organic layer was washed, brine and dried over sodium sulfate. Concentration of the solvent and purification of the resulting residue over silica gel column produced 5-carboxymethyl camptothecin, fluoroethyl ester of the formula 1 R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COCH 2 CH 2 F; mp : 220°C ; IR : 3444, 1742, 1660, 1599, 1158, cm "1 : X H NMR in (CDC1 3 , 200 MHz) : δ 8.41(s, IH) 8.20(d, J=8.4Hz, IH) , 7.90(d, J=8Hz, IH) , 7.80 (t, J=7.4Hz, IH) , 7.65 (t, J=7Hz,lH), 7.66(s,lH), 6.15-5.95(m,lH) , 5.68(d, J=16Hz, IH) , 5.25(d, J=16Hz, IH) , 4.70-4.20 (m.4H) , 4.10-3.80 (m,IH) , 3.75 (Is, IH, D 2 0 exchangeable), 3.20-2.85 (m, IH) 2.05- 1.80(m,2H), 1.05 (t, 8Hz, 3H) ; Mass (m/z) : 452 (M+1) , 408, 389, 361, 317. EXAMPLE 14 Preparation of 5-Carboxymethyl camptothecin, hydroxyethyl ester Step 1: 5-Carboxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 =COOH was prepared as described in the example 11. Step 2: 150mg of 5-carboxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOH, dissolved in 15ml of dichloroethane was treated with 0.5ml of ethylene glycol and heated the contents to 80°C for 8h in the presence of cat. amount of sulfuric acid. Reaction mixture was neutralized with pyridine and diluted with ethyl acetate. Organic layer was washed, brine and dried over sodium sulfate. Concentration of the solvent and purification of the resulting residue over silica gel column produced 112mg of 5-carboxymethyl camptothecin, hydroxyethyl ester of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOCH 2 CH 2 OH ; mp : 210°C ; IR : 3336, 1737, 1658, 1593, 1158, cm '1 : ^-H NMR in (CDC1 3 , 200 MHz) : δ 8.43 (s, IH) 8.22(d, J=8.4Hz, IH) , 7.93(d, J=8Hz, IH) , 7.84(t, J=7.4Hz, IH) , 7.68(t, J=7Hz, IH) , 7.66(s,lH), 6.15- 6.00 (m.lH), 5.69(d, J=16Hz, IH) , 5.25(d, J=16Hz, IH) , 4.50-4.20(m,2H) , 4.10-3.90-3.55 (m, 3H) , 3.45(s,lH, D 2 0 exchangeable), 3.20-3.00 (m, IH) , 2.05-1.80 (m,2H) , 1.05(t, 8Hz, 3H) ; Mass(m/z) : 450 (M+1), 432, 406, 361, 317, 218. EXAMPLE 15 Preparation of 5-Carboxymethyl camptothecin, trifluoroethyl ester Step 1: 5-Carboxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOH was prepared as described in the example 11. Step 2: 80mg of 5-carboxymethyl camptothecin of the formula 1 where R»R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOH, suspended in 15ml of trifluoroethanol and heated the contents heated to 70°C for 24h in the presence of cat. amount of sulfuric acid. Excess trifluoroethanol was distilled off and the residue was diluted with ethyl acetate. Organic layer was washed with water, brine and dried over sodium sulfate. Concentration of the solvent and purification of the resulting residue over silica gel column produced 35mg of 5-carboxymethyl camptothecin, trifluoroethyl ester of the formula 1 where R=R 1 =R 2 S -R 3 =R =R 5 =H and R 6 =COOCH 2 CF 3 ; mp : 196°C ; IR : 3434, 1757, 1733,1644, 1615,1151 cm "1 : 1 H NMR in (CDC13, 200 MHz) : δ 8.40(s, IH) 8.25(d, J=8.4Hz, IH) , 7.95(d, J=8Hz, IH) , 7.84(t, J=7.4Hz, IH) , 7.65(t, J=7Hz, IH) , 7.66(s,lH), 6.10-5.95 (m, IH) , 5.68(d, J=16Hz, IH) , 5.25(d, J=16Hz, IH) , 4.65-4.35 ( ,2H) , 4.10-3.85 (m,lH) , 3.80 (s,lH, D 2 0 exchangeable), 3.35- 3.05(m,lH), 2.00-1.75 (m,2H) , 1.05(t, 8Hz, 3H) ; Mass (m/z) : 488 (M+1), 459, 444, 361, 317. EXAMPLE 16 Preparation of 9-Nitro-5-carboxymethyl camptothecin Step 1: 9-Nitro-5-carbomethoxy camptothecin of the formula 1 where R 1 =N0 2 , R=R 2 =R 3 =R 4 =R 5 =H, R 6 =COOMe was prepared as described in the example 5. Step 2: To a methanolic solution of 120mg of 9-nitro-5- carbomethoxymethyl camptothecin was added 2ml of 0.1N sodium hydroxide solution and stirred at 25°C for lOh. Reaction mixture was acidified with dilute HCl and the aqueous layer was extracted with 10% methanol- chloroform solvent mixture. Organic layer was washed with brine solution and the solvent concentrated to a minimum volume. The resulting residue was purified over silica gel column chromatography to get 55mg of 9- nitro-5-carboxymethyl camptothecin of the formula 1 where R 1 =N0 0 , R 2 =R 3 =R 4 =R 5 =H and R 6 =COOH.; IR : 3344, 2920, 1738, 1720, 1660, 1600, 1528, 1384, 1159 ; λ K NMR in (CDC1 3 ) : δ 9.21(ε, IH) 8.53(d, J=8.6Hz, IH) , 8.46 (d, J=8.6Hz,lH), 7.91(t, J=8Hz, IH) , 7.68(s, 0.5H), 7.66(s,0.5H) , 6.19-6.03(m,lH) , 5.81(br s, IH, D 2 0 exchangeable), 5.6d, J=16Hz, IH) , 5.37 (d, J=16Hz, 0.5H), 5.27 ( d, J=16Hz, IH) , 4.01-3.859 (m, IH) , 3.25 (br s, IH, D 2 0 exchangeable) , • 3.10-2.81 (m,IH) , 1.96(m,2H), 1.02 (t, J=7Hz, 3H) . EXAMPLE 17 Preparation of 5- (1' -carboxyethyl) camptothecin, methoxyethyl ester Step 1: 5-Carbomethoxy camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOH was prepared as described in the example 11. Step 2: 200mg of 5-carboxymethyl camptothecin of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =C00H, dissolved in 2ml of dichloroethane was treated with of 0.6ml of ethylene glycol monomethylether and the contents heated to 80°C for lOh in the presence of cat. amount of sulfuric acid. Reaction was quenched with pyridine and diluted with ethyl acetate. Organic layer was washed with water, brine and dried over sodium sulfate. Concentration of the solvent and purification of the resulting residue over silica gel column produced 132mg of 5-carboxymethyl camptothecin, methoxyethyl ester of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOCH 2 CH 2 OMe; Step 3: 100 mg of 5-carboxymethyl camptothecin, methoxyethyl ester of the formula 1 where R=R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 =COOCH 2 CH 2 OMe; was dissolved on 10 ml of tetrahydrofuran and added to lithium hexamethyldisilazide solution in tetrahydrofuran, precooled to -78°C. After 20min, 0.5 ml of methyl iodide was added to it and continued stirring for 30min.more and neutralized with brine solution. The organic layer was extracted with ethyl acetate and dried over anh. sodium sulfate. Concentration of the solvent and purification of the residue over silica gel column afforded 50mg of 5- (1'carboxyethyl) camptothecin, methoxyethyl ester of the formula 1 where R=Me, OCH 2 CH 2 OMe.

Claims: C L A I M S 1 . A compound of the formula 1 , wherein, R 1 , R 2 , R 3 and R 4 are independently the same or different and represent hydrogen, hydroxy, lower alkoxy, aryloxy, lower alkanoyl, nitro, cyano, halo, carboxy, carbonyloxy, amino, substituted amino, lower alkyl, substituted lower alkyl or R and R combined together represent 0-(CH 2 ) n -O- where n=l or 2 ; R 5 represents hydrogen, lower alkyl, substituted lower alkyl, lower aralkyl, hydroxymethyl, carboxymethyl, aminomethyl, substituted aminomethyl where the amino group is mono or disubstituted in which both substituents are independent or combined together to form 5 or 6 membered cyclic ring system containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur; when R represents hydrogen, R represents lower alkoxy; carbonyloxy; cyano; nitro; thio; thioalkyl; thioaryl; amide or amino group in which the amino group can be unsubstituted, mono or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of 3 to 8 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur; phenoxy, phenyl, benzoyl or benzyl where the phenyl group can be unsubstituted or substituted with mono, di or substiuents which may be selected from halogen, hydroxy, hydroxy, alkoxy, cyano, carboxy, nitro, amido, amino or substituted amino, thioalkoxy, phenyl, benzyl, lower alkyl, or substituted lower alkyl; cycloalkyl or cycloalkyl lower alkyl where the cyclic ring may be in the range of 3 membered to 7 membered ring system containing all carbon atoms; lower alkyl groups substituted with heterocyclic rings where the heterocyclic ring system having 3 to 7 atoms contains carbons with at least one heteroatom selected from oxygen, nitrogen and sulfur; lower alkanoyl; lower alkenyl; substituted lower alkyl or substituted lower alkenyl where the substituents can be halogen, hydroxy, benzoxy, carbonyloxy, alkoxy, aryloxy, carboxyl, cyano, thio, thioalkyl, thioaryl, aryl, heteroaryl, nitro, amido or amino in which amino group can be unsubs¬ tituted or mono, or disubstituted in which both substituents are independent or combined together to form a cyclic ring system of 3 to 8 atoms containing carbon and optionally one or two heteroatoms selected from oxygen, nitrogen and sulfur or COOR' where R' represents hydrogen, lower alkyl, substituted lower alkyl, or lower aralkyl or when R represents lower alkyl; lower alkenyl; lower or alkanoyl; substituted lower alkyl; substituted lower alkanoyl; phenyl, benzyl or benzoyl in which the phenyl group may be unsubstituted or substituted; or lower alkoxycarbonyl; R represents phenyl; benzoyl; cyano; nitro; lower alkanoyl; or COOR' group in which R' represents hydrogen, lower alkyl, lower aralkyl or substituted lower alkyl. 2. A compound of the formula 13, where R 1 , R 2 , R 3 , R 4 and R 5 are hydrogen and R 6 represents COOH. 3. A compound of formula 13, where R 1 , R 3 , R 4 and R 5 are hydrogen, R 2 represents hydroxyl and R 6 represents COOH, 4. A compound of formula 13, where R 1 represents nitro; R 2 , R 3 , R and R are hydrogen andd RR 6 rreepprreesseennttss CCOOCCHH 3 . A compound of formula 13, where R 1 , R 2 , R 3 , R 4 and R 5 are hydrogen and R 6 represents COOCH 2 CH 2 OH. 6. A compound of formula 1, where R 1 , R 2 , R 3 , R 4 and R 5 are hydrogen and R 6 represents COOCH 2 CF 3 and R represents methyl. 7. Compounds of formula 1 R, R 1 , R 2 , R 3 , R 4 , R 5 and have the meaning described in Claims 1 or 6 as a mixture of two diastereomers having 20 (S) , 5 (R) and 20 (S) , 5 (S) configurations. 8. Compounds of formula 1 having 20 (S), 5 (R) configuration substantially free from the 20 (S) , 5 (S) stereoisomer, where R, R 1 , R 2 , R 3 , R 4 , R 5 and R 6 have the meaning described in claims 1 or 6. 9. Compounds of formula 1 having 20 (S) , 5 (S) configuration, substantially free from the 20 (S), 5 (R) , stereoisomer, where R, R 1 , R 2 , R 3 , R 4 , R 5 and R 6 have the meaning described in claims 1 or 6. 10. A pharmaceutical composition comprising a compound according to any one of claims 1-9, or a derivative thereof and a pharmaceutically acceptable non-toxic excipient, diluent or carrier. 11. A medicine for treating cancer, leukemia or HIV related conditions comprising claims 1-9 or a derivative thereof and a pharmeceutically acceptable carrier, diluent or solvent. 12. Use of a compound according to any one of claims 1-9 for treatment of cancer, leukemia or HIV related conditions. 13. Use of a compound according to any one of claims 1-9 for manufacture of a medicament for treatment of cancer, leukemia or HIV related conditions. 14. A method of treating cancer or leukemia comprising administering to a patient in need of said treatment an effective amount of a compound according to any one of claims 1-9 or derivative thereof. 15. A method of treating HIV viruses comprising administering to a patient in need of said treatment an effective amount of a compound according to any one of claims 1-9 or a derivative thereof. 16. A process for the preparation of compounds of formula I, 1 where R and R through R have the meaning described in claim 1, which comprises, i) reacting the compounds of formula 12, 12 where R through R have the meaning described above with a compound of the formula R ~CH 2 -J where R represents lower alkyl, substituted lower alkyl, cycloalkyl, cycloalkyl lower alkyl, lower alkanoyl, lower alkenyl, cyano, nitro, thio, thioalkyl, thioaryl, phenoxy, phenyl, benzyl or benzoyl or COOR' group in which R' denotes lower alkyl or aralkyl group and J represents halogen, trialkylsilyl, trialkyltin, triphenylphosphonium salt or its derivative, trialkyl phosphonium salt or its derivative or J may represent MgG where G denotes halogen other than fluorine, in the presence of suitable acid or base or a metal such as lithium or zinc, as per the reaction conditions, to obtain compounds of the formula 13, 13 where R 1 , R and R have the meaning given above; (ii) reacting the compounds of formula 13 where R A through R have the meaning described above and R 6 represents COOR' where R' denotes lower alkyl groups with an inorganic base followed by acidification to obtain the compounds of the formula 13, 13 where R 1 through R have the meaning described above and R 6 denotes COOH group; (iii) reacting the compounds of formula 13, where R 1 through R have the meaning described above and R denotes COOH group, with compounds of formula R' -OH where R' represents lower alkyl, aralkyl, haloalkyl, hydroxyalkyl, alkoxyalkyl or aminoalkyl, in the presence of an acid to obtain the compounds of the formula 13, 13 where R through R have the meaning described above and R 6 denotes COOR' group in which R' represents lower alkyl, aralkyl, haloalkyl, hydroxyalkyl, alkoxyalkyl or aminoalkyl; (iv) reacting the compounds of formula 13, where R through R have the meaning described above and R denotes COOH group, with amines having the formula NHR R , where R and R are independently the same or different and represent hydrogen, lower alkyl, haloalkyl, hydroxyalkyl or aminoalkyl or R 7 and Rβ are combined together to form a 5 to 8 membered ring cyclic ring system containing carbon, and at least one heteroatom selected from oxygen, sulfur or nitrogen to obtain the corresponding amides of formula 13, 13 where R 1 through R have the meaning described above and R 6 denotes CONR 7 R 8 group where R 7 or R 8 are independently the same or different and represent hydrogen, lower alkyl, haloalkyl, hydroxyalkyl or aminoalkyl or R 7 and RS combined together to form a 5 or 8 membered cyclic ring system containing carbon and at least one heteroatom selected from oxygen, sulfur or nitrogen (v) reacting the compounds of formula 13 where R 6 represents phenyl, benzoyl, cyano, nitro, lower alkanoyl or COOR' group in which R' denotes hydrogen, substituted lower alkyl, lower alkyl, or aralkyl, or a group NR 7 R 8 where R 7 and R 8 have the meaning described in step (iv) , in the presence of a base, with a reagent having the formula R-G where R is not hydrogen and G represents halogen to obtain compounds of the formula 1, R 1 where R represents lower alkyl, substituted lower alkyl, lower alkenyl, lower alkanoyl, substituted lower alkanoyl, phenyl, benzyl or benzoyl in which the phenyl group may be unsubstituted or substituted and R 1 through R have the meaning described above. 17. A process for the preparation of compounds of formula 1 which comprises, i) reacting the compounds of formula 12 12 where R 1 through R 5 represents hydrogen, with carbo¬ methoxymethylene triphenylphosphorane dissolved in toluene, in the presence of pyridine to obtain compound of formula 13, 13 where R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 represents COOMe group; (ii) reacting compounds of formula 13 where R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 represents COOMe group, with aqueous sodium hydroxide solution followed by hydrochloric acid acidification to obtain compounds of formula 13, 13 where R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 denotes COOH group; (iii) reacting compounds of formula 13 where R 1 =R 2 =R 3 =R 4 =R 5 =H and R 6 represents COOH group, with ethyleneglycol monomethylether suspended in dichloro¬ ethane solvent in the presence of concentrated sulfuric acid to obtain compounds of formula 13, 13 where R 1 =R 2 =R 3 =R =R 5 =H and R 6 represents COOH group; (iv) reacting the compounds of formula 13 where R 1 =R 2 =R 3 -=R 4 =R 5 =H and R 6 denotes COOH group, with pyrrolidine and heating the reaction mixture to reflux temperature to obtain compounds of formula 13, 13 where R 1 =R =R 3 =R 4 =R 5 =H and R 6 represents CO-N(CH 2 ) 4 ; and (v) reacting the compounds of formula 13 wheref 1 - B R s- R 3 -=R 4 =R 5 s =H and R 6 represents CO-OCH 2 CH 2 OMe, with lithium hexamethyldiβilazide in tetrahydrofuran at -70°C followed by the addition of methyl iodide to obtain compounds of the formula 1, where R 1 =R 2 =R 3 =R 4 =R 5 =H, R 6 represents CO-OCH 2 CH 2 OMe and R represents methyl group.

Inventors: AKELLA SRINIVAS S S V, AKELLA VENKATESWARLU, DUVVURI SUBRAHMANYAM, KALLA RAO VENKATESWARA, VEDULA SHARMA MANOHARA, AKELLA, SRINIVAS, S., S., V., AKELLA, VENKATESWARLU, DUVVURI, SUBRAHMANYAM, KALLA, RAO, VENKATESWARA, VEDULA, SHARMA, MANOHARA
Applicants: REDDY CHEMINOR INC, REDDY RESEARCH FOUNDATION, DR. REDDY'S FOUNDATION, REDDY-CHEMINOR, INC.
Application year: 1997
Main classification: C07D491
IPC codes: A61K31, A61P31, A61P35, C07D491