DNA INTEGRATION INTO "MYCOBACTERIUM spp." GENOME BY TRANS-COMPLEMENTATION USING A SITE-SPECIFIC INTEGRATION SYSTEM

Abstract: The present invention discloses a DNA fragment that can direct the insertion of heterologous DNA into a specific site (tRNA<Ala> gene) of Mycobacterium spp. genome. This DNA fragment includes the attachment site region (attP) and the integrase gene of the mycobacteriophage Ms6. Heterologous DNA linked to this DNA fragment can be carried into the mycobacterial genome through a site-specific integration mechanism. A new DNA integration process is disclosed that consists in providing the integrase gene on a suicide vector, that transiently produces the integrase required for the insertion of the gene or genes linked to the Ms6 attP region contained in a separated plasmid vector. This process allows the construction of highly stable recombinant mycobacteria which may be used as vaccines and/or therapeutic vehicles.

Description: DESCRIPTION DNA INTEGRATION INTO "MYCOBACTERIUM SPP . " GENOME BY TRANS-COMPLEMENTATION USING A SITE-SPECI FIC INTEGRATION SYSTEM Background Mycobacteria is a major bacterial pathogen group for man in which are included the etiological agents of tuberculosis and leprosy, respectively Mycobacterium tuberculosis and Mycobacterium leprae. Mycobacterium tuberculosis accounts for more deaths world wide than any other human pathogen. The World Heath Organization estimated that there are approximately 8 million new cases and an annual mortality of 3 million. On the other hand leprosy afflicts 10 to 12 million people in 152 countries. Leprosy is endemic in the Arctic Circle and in Africa, South-America and South-East Asia. M. bovis and M. africanum can also cause tuberculosis in humans. M. bovis causes tuberculosis in ruminants and in other animals and can be transmited to humans by ingestion of contaminated milk or meat. Tuberculosis associated with M. africanum has been diagnosed in immigrants from Central African countries. Many species of mycobacteria other than M. tuberculosis, M. africanum, M. bovis and M. leprae present in the environment can also cause human diseases. For example, pulmonary diseases may be caused by the M. kansasii, M. xenopi and M.avium complex. M. marinum, often isolated from aquaria and swimming pools, may cause chronic granulomas of the skin. On the other hand BCG, an avirulent strain of M. bovis, is widely used as a vaccine against tuberculosis. This bacillus was developed by Cahnette and Guerin who noted a change in colony morphology after a serial passage of a virulent strain of M. bovis in a patato-glycerin medium containing ox bile. The efficacy of BCG continues to be debated. BCG protects against serious and disseminated disease including meningitis in newborn babies. However, its efficiency to prevent pulmonary forms, particularly in adults, was shown to be variable. The main advantage of live vaccines is that they can persist in the host owing to their capacity to replicate, resulting in continuous exposure of the host to the vaccine antigen. Thus, it is possible that a single dose of live vaccine could represent an effective dose for the induction of a long-lasting immune response. A vast array of bacterial species have been attenuated by a variety of means in an attempt to develop both safe and effective vaccines and carrier strains for heterologous vaccine antigens. The BCG is the only bacterial live vaccine that has been extensively used in humans. In the past 40 years it has been administered to more than 2.5 billion people with very few adverse effects. BCG offers some other unique attributes for the development of live vaccine vehicles: 1. it can be given at birth or shortly after; 2. it is able to induce a long-lasting immune response; 3. it is a potent immunological adjuvant; 4. BCG can be administrated as an oral vaccine to stimulate mucosal immunity; 5. it is heat stable; BCG does not require conservation at low temperatures; 6. it is easy and inexpensive to produce. The development of genetic tools for mycobacteria has enabled the cloning and the expression of foreign genes in fast-growing M. smegmatis mycobacteria and in M. bovis BCG. Plasmids have been identified in several species of mycobacteria such as M. avium, M. scrofulaceum and M. fortuitum, though the functions of most of them are unknown. The most thoroughly studied mycobacterial plasmid was the cryptic plasmid pAL5000 isolated from a strain of M.fortuitum by Labidi and co-workers 1984. The analysis of the complete sequence of the pAL5000 revealed 5 ORF and an origin of replication (Rauzier, J., Moniz-Pereira, J., and Gicquel, B. Gene 71:315-321, (1988)). This information was essential for the construction of small shuttle vectors for E.coli mycobacteria, which include an origin of replication functional in E.coli (OriE) derived from pUC19. As a genetic marker the Tn9t93-derived aph gene was used, which confers kanamycin resistance to the transformants. Expression cassettes derived from the promoter regions of BCG hsp-60 (Thole, et al., Infect, and I mun., Vol.55, p.1466-1475 (1987)) and hsp-10 (Young, R.) genes ( M. tuberculosis 19-kDa antigen (Ashbridge, K.R et al., Nucleic Acids Res. Vol. 17: 1249.1989. Stover, K. et al., J. Exp. Med., Vol 178: 197-209. (1993)), M.fortuitum bla gene (Timm, J. et al., Mol.Microbiol. Vol. 12: 491-504. (1994)) among others, were successfully used for the expression of foreign genes in fast- growing mycobacteria and in BCG. The use of recombinant mycobacteria as multivalent vaccine vehicles requires a stable construction for a long expression of the antigen genes following the administration of the bacteria to the host. Antigenic-expressing plasmid vectors are not very stable in the absence of selection, although they reach high levels of expression of the foreign DNA. One of the possibilities for increasing the stability of recombinant bacterial strains is to use a site-specific integration system of temperate bacteriophages. By this process a heterologous DNA covalently linked to a phage DNA integration locus can be inserted into the bacterial genome and be maintained for a long time. The temperate mycobacteriophage Ms6, isolated from the strain of Mycobacterium smegmatis HB5688, is able to infect and to lysogenize some strains of M smegmatis.lt was shown that Ms6 can be used as a vehicle for the insertion of foreign DNA into mycobacteria genome. The aminoglycoside phosphotransferase gene from the Tn5 transposal was inserted into the M. smegmatis genome through lysogenization using a transducing derivative of the mycobacteriophage Ms6. It was demonstrated that Ms6 DNA can be inserted into the bacterial genome through a site-specific recombination mechanism. The DNA restriction fragment that contains the phage attachment site attV was identified and sequence analysis of this region showed that Ms6 is different from D29 and L5 mycobacteriophages. The integrative locus of mycobacteriophage Ms6 was sequenced and inserted into a plasmid vector. This recombinant plasmid was integrated into Mycobacterium smegmatis, M. bovis-BCG, M tuberculosis. The present invention was based on the site-specific integration system of the mycobacteriophage Ms6. Summary of the invention The object of the present invention is the construction of highly stable recombinant mycobacteria strains, particularly BCG, to be used as "in vivo" vectors for presenting antigens, immunomodelators and other therapeutic agents. The present invention relates to: 1. the Ms6 DNA integrating region responsible for the integration of the bacteriophage DNA into the mycobacterial genome; 2. a second DNA fragment that includes the Ms6 att? region linked to an expression cassette composed of the relevant DNA sequence (which may encode a variety of heterologous genes, including genes for antigens which protect against several pathogens, lymphocynes and other therapeutic agents) under the control of a promoter region; 3. a third DNA fragment that carries the integrase gene under the control of a strong promoter region originating from the bacteriophage Ms6; 4. a new method of integrating DNA based on the transient production of integrase sufficient for directing the integration of the relevant DNA sequence linked to the attP region into the bacterial genome; 5. the expression of vaccine-stable vehicles which are such recombinant strains of mycobacteria produced by this new integrating process capable of continuously expressing the relevant DNA fragment. The Ms6 DNA integrating region (attP-int) includes: the phage attachment site (<3fttP) with the bacterial genome and the gene of the integrase, which encodes the enzyme that mediates the recombination between the αttP and a corresponding attB site of the bacterial genome. This mechanism is called site- specific integration because it only occurs in a particular site of the bacterial genome. A common core sequence is present in both the αttP and αttB regions and is the site where the DNA exchange occurs. This recombinational event leads to the duplication of the comon core sequence which will flank the inserted DNA. The DNA integration process of the subject invention has important advantages over the integrating systems that are presently available. In particular, an improvement of the transformation efficiency of mycobacteria due to the use of a bacteriophage integrase in the integration process. This differs from the process that is presently available in which the DNA fragment is integrated into the bacterial genome by a double homologous recombination mechanism which, in slow growing bacteria such as BCG, occurs with a very low frequency, resulting in poor transformation efficiency. An integrative vector based on a mycobacteriophage attP-int region linked to an expression cassette is available to construct recombinant mycobacteria strains. However, considering that in bacterial lysogens the excision process of the prophage requires the contribution of the integrase and the excisionase, it is possible to improve the stability of the recombinant strains by eliminating the integrase gene from the integrative vector. This is the subject of the present invention. A new process is proposed which eliminates the integrase gene from the integrative vector and therefore from the genome of the recombinant mycobacteria produced to guarantee the stability of the recombinant strains. This novel integration process consists in transforming the mycobacterial cells simultaneously with two plasmids. Plasmid 1 includes the integrase gene under the control of a strong promoter region and it is not capable of replication because it does not have a mycobacterial origin of replication. Plasmid 2 contains the αttP region linked to a relevant DNA which is under the control of mycobacterial gene regulation signals. The supply of the integrase gene in an independent suicide vector produces enough integrase to direct the integration of plasmid 2 which carries the αttP region and the relevant DNA. It was confirmed that this process of integrating relevant DNA into the bacterial genome produces recombinant mycobacterial strains that do not lose the exogenous DNA following long non-selective growth. Therefore, these highly stable recombinant mycobacterial strains can be safely used as vehicles to express, following administration to humans or animals, a gene or genes of interest. These recombinant mycobacterial strains can be used as vectors for presenting one or more protective antigens, against one or more pathogenic agents. These mycobacterial vehicles can also be used to express immunopotentiators, cytosines, anti-tumour and anti-cancerous agents and other useful polypeptides and proteins. The integrative vectors and the integrating process described in this invention have made it possible to insert exogenous DNA into the genome of a wide range of Mycobacterium species, including: M.smegmatis, M.vaccae, M. tuberculosis, M. bovis-BCG.The scope of the present invention is not restricted to mycobacteria and is applicable to other bacteria, such as: Samonella ssp., Vibrio spp., Shigella spp., Lactobacillus spp., Streptomyces spp., Corynebacterium spp., Listeria spp.. Detailed description of the invention The invention will now be described with the respect to the following examples. However, the scope of the present invention is not limited thereby. Example 1 A. Identification of the attP-int region of the mycobacteriophage Ms6 DNA The site-specific integration locus of the mycobacteriophage Ms6 DNA was previously pinpointed to a 4.8-kb Bglll restriction fragment. This fragment was cloned in a pUC 19-derived plasmid vector and the recombinant plasmid generated was named pAV8800 (Figure 1) and electroporated into M. smegmatis. The DNA of the transformants was analysed by the Southern/hybridization method in order to test the integration of the recombinant plasmid. In all the transformants analysed the same pattern of bands was founds, indicating a site-specific integration mechanism. The αttP phage attachment region was located in a 0.8-kb Bglll-Sall fragment at the far left of the 4.8 kb Bglll fragment (Anes, et al., 1997) (Fig.2). Considering that in the majority of the procaryotic site-specific integration systems the integrase (ini) gene is close to the attP, the DNA stretch between Bglll and Sspl that contains the Ms6 attP region was sequenced (Fig.3). The base composition of this region is 63% G+C, which is practically identical to other mycobacterial genetic elements. An ORF of 1119 bp starting in an ATG initiation codon at the position 697 and ending in a stop codon at the position 1815 was identified. The deduced protein of 372 amino acids was aligned with other integrases (Fig 4). Although integrases have poor homology among their amino acid sequences, the carboxy-terminal regions contain amino acids which remain in specific positions. The alignment revealed the presence of an arginine in the middle of the first domain, as well as the histidine, arginine and tyrosine residues in the second domain, which is a characteristic shared by all members of the integrase family. This observation shows that this ORF encodes the integrase of the bacteriophage Ms6. A threonine residue in the first domain is also present in all the integrase sequences represented in Figure 4, whereas this characteristic is absent in some bacteriophage integrase-related proteins. The attP-int region of Ms6 DNA is limited by two inverted repeat sequences. At the 3' terminus of the int gene lies a structure resembling a rho- independent transcriptional terminator that can form a hairpin secondary structure followed by a poly T-rich sequence. At the 480 position (Fig.3) a second inverted repeat is located which can form a 10-bp stem loop, but no poly T-rich sequence is present close to it. Preliminary functional experiments showed that this inverted repeat is required for integration. As in other integration systems, this inverted repeat may belong to the ttP region and may be a site that is recognized by the integrase. B. Identification of the DNA sequences of the phage and bacterial attachment sites The common sequence where the recombination between the phage DNA and the bacterial genome occurs was determined after the alignment of 4 DNA fragments: 1-2) the right and the left host-phage DNA junctions (αttR and attL), 3) the bacteriophage attP-int region and 4) the attachment site in the bacterial genome αttB (Fig.5). To identify the host-phage DNA junctions, a λEMBL4 library was constructed by molecularly cloning the genome of an M. smegmatis (pAV8800) transformant. A recombinant phage containing the complete pAV8800 sequence, inserted within an EcoPl bacterial restriction fragment, was analysed by Southern/hybridization using a 0.8-kb Bgll -Sall fragment, which contains the phage attachment region, as a probe. Two Sail restriction fragments with respectively 0.9 kb and 1.2 kb containing the putative αttR and αttL sites were identified and sequenced. The αttB region was directly amplified by PCR from the M. smegmatis DNA, using specific oligonucleotides pi (5'- CGCTGTTGGTGGGCCTGATCG-3') and ml (5'-ACACGCCGGATGAATGACC- 3') designed from the right and left junction sequences. The alignment of the αttR αttL, bacteriophage αttP region and αttB sequences revealed a 26 bp common core sequence where the recombination between the phage DNA and the bacterial genome occurs (Fig.5). C. Identification of the bacterial genome region where the atiB sequence is situated. In M. smegmatis genome the common core region lies at the 3' end of a sequence that has 83.8% homology with the tRNA^ 3 gene of Micrococcus luteus and 76% with the tRNA^ 3 gene of E. coli. Moreover, this target sequence possesses, as do all the tRNA^ 3 genes studied to date, a characteristic base pair (G3 - U70) in the amino acid acceptor stem of the tRNA structure (Fig.6), (19). These observations indicate that in lysogenic strains Ms6 DNA is inserted at the 3' terminal sequence of the M. smegmatis tRNA^ 8 gene. The common core sequence is also found in the cosmid cSCY22D7 of the genome library of M.tuberculosis H37Rv supplied by Dr. Stewart Cole and his colleagues at the Institut Pasteur and it has been sequenced by the Sanger Center. The sequence analysis of the homologous segment in the M. tuberculosis genome indicates that both tRNA genes are present within a similar organization. However, there are two altered bases which lie within the D loop (a T to C change in M. tuberculosis) and the TΨGC loop (G instead of A) as shown in Fig.6. Nevertheless, this G to A change within the core region does not appear to have any significant effect on the efficiency of integration as determined by the transformation frequencies. D. Construction of the integrative shuttle vector pAVl The 4.8 kb Bglll restriction fragment which encodes the integrative functions of the mycobacteriophage Ms6 was cloned into the pUC19 derived plasmid, pZM3, which carries the aph gene that confers resistance to Tn5 kanamycin. The new plasmid named pAV8800 (Fig. l) was electroporated into smegmatis, M.vaccae and BCG. The results of the transformation efficiency expressed as the number of kanamycin-resistant transformants per μg of DNA are shown in Table 1. The stability of the M. smegmatis recombinants produced was tested. After growth in a non-selective medium for 50 generations, 95% of the clones remained kanamycin-resistant. However, none of the 4 BCG recombinants obtained survived new cultures in selective or non-selective media. Southern/hybridization studies confirmed that the plasmid sequences are integrated in a specific site of the bacterial genome in all the M. smegmatis transformants analysed. In order to improve the stability of the recombinant mycobacteria and, on the other hand, to demonstrate that the ttP sequence and the int gene are sufficient for directing site-specific integration into the mycobacteria genom, the attP-int region was produced by PCR directly from Ms6 DNA. Using the initiating sequences C2 (5'-GATCCGGGCCTCGTACTCG-3') and PRPVA10 (5'-CAACCCGACGGTGTTGCG-3') a fragment of 1818 bp containing the integrative functions was produced and was then cloned in the plasmid vector pCR3 (invitroge ). The recombinant plasmid produced, pAVl (Fig.7), replicates extrachromosomally in E.coli but not in mycobacterial cells because it does not contain any mycobacterial origin of replication. pAVl was electroporated into M. smegmatis, M. vaccae, BCG, M. tuberculosis H37Ra. The electroporation process used in all the experiments described in this invention was carried out as follows: mycobacterial cells were grown in 500 ml of Myco Broth (Middlebrock 7H9, Nutrient Broth of Difco, 0.5% glucose and 0.05% Tween 80) to OD 600 0.8, harvested by centrifugation, washed 3 times with 500ml of cold 10% glycerol and finally resuspended in 2 ml of cold 10% glycerol. 1 μl of DNA was added to 40 μl of cells in an ice-tray with a 0.1 cm gap between the electrodes and pulsed in a Bio-Rad Gene Pulser device. A single pulse at 1.8 kV and 25 μF was given with the Pulse Controller resistance at 200 Ohms. The samples were diluted in 5 ml of broth and incubated for 1 hour at 37°C for expression of the antibiotic- resistant gene. Cells were concentrated and plated on a Myco agar (Myco Broth with 1.2 % of Bacto Agar) medium containing 15 μg/ml of kanamycin. The number of transformants per μg of DNA was respectively 1.0x10 and 3x10 in M. smegmatis and BCG/ tuberculosis (Table 1). These transformation efficiency values are similar to those obtained with pRR3 (Ranes, M.G., J. Rauzier, M. Lagranderie, M. Gheorghiu, and B. Gicquel, J. Bacteriol. vol. 172:2793-2797.1990), a shuttle plasmid that replicates extrachromosomally in Mycobacterium spp and E. coli. Table 1. Transformation efficiency of Ms6-derived integrative vectors Transform ants/μg of DNA Stain pAV8800 pAVl pRR3 M. smegmatis mc 2 155 1.3 x 10 4 1.0 x 10 5 >10 5 M. vaccae 1.2 x 10 4 ND ND BCG Pasteur 4 4 x 10 3 4 x 10 3 M. tuberculosis H37Ra ND* 3 x 10 3 4 x 10 3 *ND- Not done The plasmid pAV8800 which contains 4.8 kb Bglll gives a lower number of transformants (1.3 X 10 4 ) in the mc 2 155 strain than pAVl (1.0 x 10 5 ). In BCG the difference in the transformation efficiency between these two plasmids is bigger, 3 logs. The explanation for this behaviour has not yet been found, although the answer can be obtained when the functions encoded in the DNA segment at the 3' end of the attP-int region were revealed. Disruption of the ORF present in the Ms6 integrative region destroys the ability to transform mycobacteria. The deletions and insertions performed at the 3' end of the int gene lead to non-integrative activity. Southern/hybridization analysis confirmed that the Ms6-derived integrative plasmid (pAVl) was inserted into the same site (αttB) in all transformants examined (Fig.8). Chromosomal DNA from three independent Km R transformants and from pAVl were cut with BamHI and hybridized with the radiolabelled Ms6 αttP region. This probe did not hybridize with chromosomal DNA from M smegmatis (Fig. 8 A, lane 4), while two hybridizing bands of 14 kb and 0.6 kb respectively were visualized in the chromosomal digests of all the Km R transformants (Fig. 8A, lanes 1, 2 and 3). The 0.8 kb BamHI restriction fragment, derived from pAVl, containing the αttP region, is intact in lane 5. In Fig.8B hybridization with the αttB probe (a 0.5 kb DNA segment M smegmatis, obtained by PCR and cloned into pCR3) revealed a 13.8 kb fragment in the M.smegmatis DNA digests (lane 4), which is absent in all transformants. In turn, in these digests the two hybrid fragments (14 kb and 0.6 kb) were detected (lanes 1, 2 and 3). The sizes of the two hybridizing bands were consistent with the prediction for integration of a pAVl copy into the M.smegmatis genome at the attachment site (αttB) present in the 13.8 kb BamHI fragment (Fig.8B, lane 4). The 6 kb restriction band in Fig.8B corresponds to common sequences present in both pCR3 and pAVl plasmids. The 3 kb fragment found in all M.smegmatis DNA digests possibly corresponds to another region that contains sequences with high homology with this probe. pAVl was also stably integrated, by a site-specific integration mechanism, in the BCG (Fig.9 A and B) and M. tuberculosis H37Ra genomes (Fig.10). In these slow-growing mycobacteria the plasmid integration target hybridizes with the M.smegmatis attB sequence. However, the sequences that are linked to the tRNA^ 3 gene of M. tuberculosis are completely different from those of M. smegmatis. As a consequence of this, only one hybrid band with 2.4 kb (Fig.10, lanes 3, 4 and 5) can be detected by Southern/hybridization analysis of the DNA of H37Rv transformants using the αttB region of M.smegmatis as a probe. It can be concluded that the pAVl integration site in the genome of the fast and slow-growing mycobacteria consists of the last 25 bases of the tRNA^ 3 gene (Fig.6). E. Stability of the recombinant mycobacteria The stability of the M. smegmatis transformants carrying a plasmid inserted in their genome was evaluated as follows: The recombinant cells were grown to saturation in broth with kanamycin. The cultures were then diluted to 1/10000 into a broth without kanamycin and grown to saturation. Two further cycles of dilution and growth were carried out, corresponding to a total number of 50 generations of bacterial growth. The cultures were diluted so that 0.1 ml of the bacterial suspension plated out on a non-selective medium gives nearly 300 single colonies per plate. 100 of these colonies were patch plated onto both selective and non-selective media plates. The % of kanamycin-resistant colonies corresponds to the fraction of the cell population that maintains the plasmid integrated in the genome. 95% of the M. smegmatis cells conserve the plasmid pAV8800 integrated in their genomes after 50 generations in a non-selective medium. By contrast, in BCG pAV8800 is not stable. pAVl, which includes only the attP-int region, can be safely maintained in the M. smegmatis and BCG genomes after 50 generations without kanamycin. Table 2. Stability of the integrative plasmids Mycobacteria spp. plasmid % kanamycin-resistant colonies M. smegmatis mc2155 pAV8800 95 pAVl 100 pAV3 100 M. bovis BCG pAV8800 0 pAVl 97 Despite the excellent stability of the pAVl transformants of BCG, for safety reasons a new integrative vector was constructed, pAV3 (Fig. 11), which only contains the Ms6 αttP region, meaning that the integrase gene was excluded. Therefore, the int gene will not be present in the genome of the recombinant mycobacteria produced to guarantee the stability of the genetic construction. The integrase required for recombination is provided by a suicide vector . The stability of the transformants containing the pAV3 inserted in the genome is 100%, in both. M.smegmatis and BCG. Example 2 Construction of integrative expression plasmids derived from the att-int locus of the mycobacteriophage Ms6 A. Elimination of a BamHI site of the int gene by directed mutagenesis To facilitate future manipulations, the restriction site BamHI in the int gene was eliminated by means of a point mutation. The cytosine in position 769 of an He codon was changed into a Tymidine (ATC/ATT). This point mutation does not change the amino acid to be inserted in that position since the ATT codon also encodes an He residue. This point mutation was carried out using Stratagene's QuickChange™ Site-Direct mutagenesis kit. The initiating sequences used to perform this point mutation had the following base sequence: pA: 5'-GTGGTGCCGAATCCATTC-3' and pT: 5'-GCCGAATGGATTCGGCACCAC-3\ The underlined bases are responsible for the point mutation. This technique was utilized to eliminate the BamHI site in position 820, in the int gene, of the plasmid pAVl resulting in the cloning plasmid pPAl (Fig.12), which contain a single BamHI restriction site. Construction of plasmids including mycobacterial promoter expression cassettes. Plasmid pPAl was digested by EcoRI in order to separate by gel electrophoresis the EcoRI fragment which contains the attP-int region. This fragment was attached to the plasmid pZM3 previously digested with the same restriction enzyme to create pZM4 (Fig.13). This plasmid was digested with Kpn and the ends were filled with the T4 polymerase and then digested with Hindlll, resulting in DNA with blunt ends. Expression cassettes, including promoter and translation initiation signals, of BCG hsp60, M.tb 19kDa protein and a hybrid comprising the Hsp60 promoter and the translational signals of the M. tb alfa antigen genes were inserted between restriction sites Hindlll and Xbal of pZM4 to produce the plasmids pAV5 (Fig.14), pAV6 (Fig.15) and pAV7(Fig. l6). These expression cassetes were removed respectively from plasmids pSMT3, pAU151 and pDE22 constructed by Peadar O'Gaora et al., 1994. First, the plasmids were digested with Xbal and the Xbal ends were flushed with T4 DNA polymerase. Secondly, a Hindlll digestion was carried out to produce small DNA fragments blunt Hindlll ends containing the expression cassettes. These fragments were attached to the blunt Hindlll ends of the linear plasmid ZM4. Relevant DNA sequences can be fused with one of these expression cassettes using the multiple cloning site located at the 3 ' end. Example 3 DNA integration by trans-complementation A. Construction of the integrative plasmid pAV3 The minimal functional attP region of the Ms6 was defined by performing a series of deletions at the 5' end of the att-int fragment used to construct the plasmid pAVl and checking for the capacity of each deletion to direct the integration into the M. smegmatis genome. The deleted fragments were obtained by PCR using the following initiating sequences at the 5' end: pC2 5'-GATCCGGGCCTCGTACTCG-3' pl3 5'-CAGCAGACACCCACATGTCCG-3' plC 5'-CGCTACAGGTCTAAAAAGGTCGG-3' pC 5 ' -AGGGGTTCGAATCCCCTTAGCTCCAC-3 ' At the 3 ' end the primer plO, 5'-CAACCCGACGGTGTTGCG-3' was always used. Therefore, with the pair of primers pC2/plO a fragment of 1818 bp was obtained; with the pair of primers pl3/pl0 a fragment of 1521bp; with the pair of primers plC/plO a fragment of 1459 bp and with the pair of primers pC/plO a fragment of 1382 bp. All these PCR products were cloned into the pCR3 plasmid and electtoporated into M. smegmatis cells. The transformation efficiency, the number of kanamycin-resistant colonies/μg of DNA of each plasmid was determined and the results are shown in the Table 3. Tabela 3. DNA fragment (bp) Transformation efficiency 1818 1.10 5 1521 1.10 5 1459 0 1382 0 The results of the transformation efficiency show that the fragment of 1521 bp is the smallest functional integrative cassette. The fragment 1459 bp with 62 bp less is not functional which means that in this short piece of DNA there are sequences which are important for site-specific recombination. A recombinant plasmid containing the αttP region of the bacteriophage was constructed. The αttP region was obtained by direct amplification from the phage DNA, using PCR and the pair of primers pl3/p2N (5'-GTTCCGTCTTTGCGGACCC-3'). The amplicon of 273 bp was cloned into the plasmid pCR3 to produce the plasmid pAV3 (Fig.11). B. Construction of the suicide helper vector pAV4 Insertion of a strong promoter region upstream of the t ' «t gene. A strong promoter region (PI) from the mycobacteriophage Ms6 was identified by inserting Sau3Al restriction fragments into the multiple cloning site of the promoter probe plasmid pJEM15 described in J.Timm et al., J.Bacteriol. Vol.176. p.6749-53 (1994). The DNA sequence of this promoter region was deteπnined and is represented in Fig.17. A suicide plasmid vector containing the int gene under the control of the PI promoter was constructed as follows: The plasmid pMGI which contains the LacZ gene of E.coli under the control of the PI promoter was digested with the Kpnl and Seal restriction enzymes in order to separate the PI promoter region. The DNA fragment containing PI was attached to the plasmid CMV (Stratagene), double digested Kpnl and Smal to form the plasmid pAV4800 (Fig.18). The integrase gene was obtained by PCR from the phage DNA using primers p661 (5'- CAGCACCGATCTAGACACAACGGAT-3 which generates a restriction site in the the product PCR (amplicon) and pl896 (5 , -TCCGCACAAGCTTCTGCGGAACCTGG-3 , l which generates a Hindlll restriction site in the other end of the amplicon. This amplicon was inserted into the plasmid pCR3 (Invitrogen), resulting in two different plasmids with respect to the orientation of the amplicon in the multiple cloning site (mcs). The plasmid in which the Hindlll site of the amplicon is side by side with the Hindlll site of the mcs was chosen, pAV 6300/«t (Fig.18). The Hindlll, Xbal fragment which encodes the integrase of the pAV6300 was inserted between the Hindlll and Xbal restriction sites of the plasmid pAV4800 to form plasmid pAV1600H3. Finally, the Kpnl fragment, carrying the Pl- «t gene, was cloned into the Kpnl site of the E.coli plasmid pUC18 to generate the plasmid pAV4 (Fig.18). C. Experiment of trans-integration To improve the stability of the recombinant mycobacteria a site- specifc integration process was developed using the int gene in a suicide vector and the αttP region in another plasmid in association with the foreign gene(s) to be expressed. With this process the integrated DNA fragment does not codify either the integrase or the excisionase functions, which are both required for the excision process. Therefore, the probability of excision is reduced to a very low level which allow us to expect that the foreign DNA will be maintained forever. A first recombinant plasmid, referred to as the helper vector (pAV4), which does not contain any bacterial origin of replication (suicide vector) was used to transiently produce the int protein required for mediating the integration process. The helper vector includes 1) the int gene under the control of a strong PI mycobacterial promoter; 2) the E.coli plasmid vector pUC18. A second recombinant vector, referred to as the integrating vector (pAV3), which was used to introduce the relevant DNA into a specific site of the mycobacterial genome. The integrating vector includes: 1) the αttP region of the Ms6 mycobacteriophage; 2) DNA that encodes a selective marker of mycobacterial origin; 3) DNA that encodes a strong and regulated mycobacterial promoter region to control or direct the expression of the relevant DNA; 4) the relevant DNA that encodes vaccinal antigens, immunomodulators and polypeptides or proteins of interest. To produce a stable recombinant mycobacteria, the helper and the integrating plasmids were mixed and electtoporated into mycobacterial cells (this system applies to both fast and slow-growing mycobacteria including BCG). The transformed cells were plated onto a medium containing kanamycin. The kanamycin-resistant transformants can be isolated with a transformation efficiency of about 4.10 4 colonies/μg of DNA. The presence of the integrative plasmid within the bacterial genome was confirmed using a Southern/hybridization using the αttP region as a probe.The stability of the recombinant mycobacteria produced was 100% (all the ttansformants conserved their kanamycin-resistance after 50 generations in a non-selective medium). This experiment demonstrated, for the first time, that it is possible, using the site-specific integration system of mycobacteriophage Ms6, to integrate DNA into the mycobacteria genome using a trans-complementation process. Example 4 Construction of an integrative expression vector based on the Ms6 PI promoter The PI promoter region of the bacteriophage Ms6 strongly expresses the LacZ reporter gene at high levels in both fast and slow-growing Mycobacterium spp, including BCG. This ttanscriptional fusion, carried out by the plasmid pMGl, includes the PI promoter and CII-LacZ hybrid gene (fusion between the ttanslation initiation signals of the CH gene of the bacteriophage λ with the E.coli LacZ gene at its 5' truncated end). Figure 19 shows the DNA sequence of this construction. A DNA fragment (cassette) encoding the Pl-CII-LacZ expression conttol sequences (i.e. promoter and translation signals) was produced by PCR using the primers pm4 (5' end) and pm2 (3' end) and, as the DNA target, the plasmid pMGl. The primers have the following base sequences: pm4: 5 ' -CCTTTAATAGATTATATTACTAATTATTGGGG-3 ' pm2: 5'-TCCGATTCGTAGAGCCTCG-3 ! The amplicon was cloned into the plasmid pCR™3 (Invitrogen) and the resulting plasmid was digested with EcoRI. The short EcoRI fragment of 446 bp was purified by gel electrophoresis and its extremities were filled with the T4 polymerase. This blunt-ended DNA fragment that encodes expression cassette was inserted into the Sαcl site, previousely flushed with T4 polymerase, of plasmid the pZM5 (Fig.20). The plasmid pZM5 was obtained from the insertion of the pAV3 EcoRI restriction fragment, which contains the αttP sequence of the phage Ms6, into the EcoRI site of the plasmid ZM3 (Fig.20). The resulting integrative expression plasmid was named pZM6 (Fig.20). Figure 21 represents the DNA sequence of the pZM6 expression cassette and the multiple cloning site. Gene fusions with the 5' end of the CII-LacZ gene can be obtained by inserting the DNA to be expressed into the multiple cloning site of the plasmid Zm6. These fusions will be under the conttol of the PI promoter region. The plasmid pZM6 can mediate the integration, by trans-complementation and the expression of gene fusions in Mycobacterium. The integrative expression plasmid vector described in the ttans- complementation technique can also cany other mycobacterial promoter regions such as: BCG HSP60, the 19kDa antigen of M.tuberculosis or of the M. fortuitum bla gene. Example 5 Cloning of antigen genes in expression cassettes A series of antigen genes can be expressed by BCG using the metodology developed in this application. Expression cassettes were constructed by PCR and cloned into the multiple cloning site of pZM5 (Fig.20). The following antigen genes can be fused with pZM6-derived expression cassettes: HIV-1 : env; gag; fransmembrane gp 41gene; vif HIV-2: env; gp36; gag; vif. FIV: env glicoproteins; gag polyprotein; vif. Hepatitis B Virus: pre-S l; pre-S2 and S antigens Leishmania: Surface glycoproteins GP 63 and GP43; proteins LNP18 and P32. It is to be understood, however, that the scope of the present invention described above is not to be limited to the specific embodiments described. The invention may be put into practice other than as specifically described and still fall within the scope of the accompanying claims.

Claims: CLAIMS 1. A genetic system for integrating DNA into a specific site of the Mycobacterium spp. (or bacterium) genome comprising: a) a first DNA containing the integrative functions (IF) of a bacteriophage linked to a relevant DNA, which encodes peptides, polypeptides or proteins, to be expressed under the control of a promoter region; or, b) two plasmids: 1) an integrative plasmid DNA carrying the phage attachment site (αttP) and the relevant DNA to be expressed under the control of a promoter region and 2) a helper plasmid DNA that encodes an integrase. 2. An integrative DNA of Claim 1 a) which encodes the DNA integration of the mycobacteriophage Ms6 into the tR A^ 3 gene of the Mycobacterium spp. genome and includes: the bacteriophage attachment site (αttP) and the integrase gene. 3. An αttP sequence and an integrase gene selected from the group consisting of Fig.3 and derivatives thereof, which encodes the site-specific integration mechanism into mycobacteria genome. 4. An integrative plasmid comprising : a) the first DNA sequence of Claim 1 a) b) the relevant DNA under the control of a mycobacterial promoter region c) which includes an origin of replication for E. coli and d) a selective marker for mycobacteria. 5. A DNA sequence that promotes the expression of DNA in mycobacterial cells selected from the DNA sequence shown in Fig.17 and derivatives thereof . 6. An integrative plasmid vector of Claim 1 b) comprising : a) the αttP region b) the relevant DNA under the control of a mycobacterial promoter region c) an origin of replication for E. coli d) a DNA encoding a selective marker for mycobacteria. 7. A suicide helper plasmid vector of Claim 1 b) (unable to replicate in mycobacterial cells) comprising: a) The integrase gene under the control of the promoter region encoded by the DNA of Claim 6, or another mycobacterial promoter b) an origin of replication for E.coli 8. A DNA integration process by trans-complementation which consists in electtoporating Mycobacterium cells with a mixture of the integrative and helper suicide plasmids, Claims 6 and 7 or derivatives thereof. This process can be adapted to other bacteria such as: E.coli, Salmonella spp., Vibrio spp., Shigella spp., Listeria spp., Streptococcus spp., Lactobacillus spp., Corynebacterium spp., Streptomyces spp. 9. A genetic system of Claim 1 which directs DNA integration into Mycobacterium tuberculosis, M. bovis-BCG, M. smegmatis, M.vaccae, M. avium, M. intracellulare, M. fortuitum, M. genovences and other Mycobacterium spp and genetic variants thereof. 10. A recombinant mycobacteria comprising a foreign relevant DNA under the conttol of a mycobacterial promoter region inserted into the genome by a site-specific integration mechanism directed by the DNA of Claim 1 and/or derivatives thereof. 11. A vaccine comprising: the recombinant mycobacteria of Claim 10; and an pharmaceutically acceptable carrier.

Inventors: DA COSTA GARCIA MIGUEL ANGELO, DA SILVA ALVES PAULO JORGE, FRAZAO MONIZ PEREIRA JOSE ANTO, FREITAS VIEIRA ALCINO, RIBEIRO DOS SANTOS ANES ELSA M, DA COSTA GARCIA, MIGUEL, ANGELO, DA SILVA ALVES, PAULO, JORGE, FRAZAO MONIZ PEREIRA, JOSE, ANTONIO, FREITAS VIEIRA, ALCINO, RIBEIRO DOS SANTOS ANES, ELSA, MARIA
Applicants: DA COSTA GARCIA MIGUEL ANGELO, DA SILVA ALVES PAULO JORGE, FRAZAO MONIZ PEREIRA JOSE ANTO, FREITAS VIEIRA ALCINO, MEDINFAR PRODUTOS FARMACEUTICO, RIBEIRO DOS SANTOS ANES ELSA M, DA COSTA GARCIA, MIGUEL, ANGELO, DA SILVA ALVES, PAULO, JORGE, FRAZAO MONIZ PEREIRA, JOSE, ANTONIO, FREITAS VIEIRA, ALCINO, LABORATORIO MEDINFAR-PRODUTOS FARMACEUTICOS, LDA., RIBEIRO DOS SANTOS ANES, ELSA, MARIA
Application year: 1997
Main classification: C12N15
IPC codes: A61K39, C12N1, C12N9, C12N15