A HYDROCOLLOID WOUND GEL

Abstract: A hydrocolloid gel composition, especially a wound gel composition, which comprises: a) a water insoluble, water swellable cross-linked cellulose derivative; b) an alginate; and c) distilled water in a sufficient amount to make up the difference between the amount of ingredients a) + b) and 100 % and wherein the gel comprises from 0.05 to 5.00 % by weight of alginate, shows better absorption capacity than known gels, accelerates the healing by serving as a slow-release system for active ingredients promoting wound healing.

Description: TITLE A hydrocoUoid wound gel FIELD OF THE INVENTION The present invention relates to a hydrocoUoid wound gel It also relates to a 5 wound dressing containing a hydrocoUoid wound gel and a method for preparing a hydrocoUoid wound gel useful for cleansing and debridmg wounds a dressing containing a hydrocoUoid wound gel useful for cleansing and debridmg wounds as well as a method for treating a wound comprising applying a hydrocoUoid wound gel to a wound, in particular for filling of cavities of wounds or applying a o dressing containing a hydrocoUoid gel over the wound as well as the use of the hydrocoUoid gel as vehicle for transplantation of cells BACKGROUND OF THE INVENTION It is well known that when treating chronic and acute wounds like venous stasis ulcers pressure sores, open surgical wounds and bums it is a primary goal to re- 5 duce the healing time Speeding-up of the healing may be obtained by cleansing and debridmg of the wound combined with an effective removal of wound exu- date Commonly used wound dressings comprise gauze, foams, sponges, cotton pads or other fibrous materials Gauze and other fibrous materials may absorb fluids by capillary action but implies, however the disadvantage that fibres of the 0 gauze or other fibrous materials may adhere to the new tissue causing damage to the newly formed tissue when removing the gauze or fibrous material causing wound injury on removal Amorphous hydrogels is a category of products used for debπdement These gels function by keeping the wound moist and thereby enhancing autolytic debπ- 5 dement of necrotic tissue by enzymes generated in the body by e g inflammatory cells A too aggressive debπdement can impair the healing as some of the active components may be cytotoxic Furthermore, other ingredients of a gel, e g preserving agents or active agents or other constituents may also be cytotoxic and may hamper the healing Often polyols are used for amorphous hydrogels as bacteπostatic agents, but are known for being potential allergenic, see e g Journal of Pharmaceutical Sciences 5 1990,79 312-316, Arch Dermatol 1979,115 1451 , CUTIS, 1978,21 166-178, and Contact Dermatitis 1980,6 341-144 EP 0 567 311 A2 discloses a hydrocoUoid wound gel composition which is stated to cleanse and debride wounds and to have some exudate absorbing capacity, said gel containing from about 0 005% to 1 0% by weight of a pectin, from about 10 2 0% to 4 5% sodium carboxymethylcellulose, from about 15 0% to 20 0% by weight of propylene glycol and the remainder substantially water to make 100% by weight EP 0 576 523 B1 discloses a wound dressing comprising a gel containing a water-insoluble, water swellable cross-linked cellulose derivative, water and a poiyol 15 component wherein the cellulose derivative comprises less than 10% by weight of the gel The dressing may contain additional debridmg agents, e g enzymatic debridmg agents and/or growth factors EP 0 512 855 A2 discloses an absorbent wound filler comprising a polymeric matrix containing one or more styrene radial or block copolymers, one or more po- 20 lyisobutylenes and mineral oil and absorbing powders comprising sodium/calcium alginates, optionally cross-linked sodium carboxymethylcellulose, optionally absorbent polyacrylates and optionally water soluble hydrocolloids The role of the matrix is to hold the absorbing powders and the polyisobutylene helps to bind the powders in the polymeric network and mineral oil is a plasticizer 25 for the styrene radial or block copolymer component WO 95/17166 discloses a wound hydrating gel comprising a hydrocoUoid system comprising carboxymethylcellulose, sodium alginate/calcium alginate and a preservation system in which is preferably used a self-gelling Na/Ca alginate having about 6-7% sodium minerals and about 2 5-3 5% calcium minerals BRIEF DESCRIPTION OF THE INVENTION The present invention relates to an amorphous hydrocoUoid gel composition comprising a water insoluble, water swellable cross-linked cellulose derivative and an alginate The invention also relates to a dressing comprising an amorphous hydrogel containing a water insoluble, water swellable cross-linked cellulose derivative and an alginate Furthermore, the invention relates to a method for preparing a hydrocoUoid gel composition comprising a water insoluble, water swellable cross-linked cellulose derivative and an alginate The invention also relates to a method for preparing a dressing containing an amorphous hydrocoUoid gel composition comprising a water insoluble, water swellable cross-linked cellulose derivative and an alginate useful for cleansing and debridmg wounds Further, the invention relates to the use of an amorphous hydrocoUoid wound gel composition comprising a water insoluble, water swellable cross-linked cellulose derivative and an alginate for cleansing and debridmg wounds as well as a met- hod for treating a wound comprising applying a hydrocoUoid wound gel composition comprising a water insoluble, water swellable cross-linked cellulose derivative and an alginate to a wound, in particular for filling of cavities of wounds or applying a dressing containing a hydrocoUoid wound gel composition comprising a water insoluble water swellable cross-linked cellulose derivative and an alginate over the wound Still further, the invention relates to the use of an amorphous hydrocoUoid wound gel composition comprising a water insoluble, water swellable cross-linked cellulose derivative and an alginate as vehicle cells to be transplanted onto a wound BRIEF DESCRIPTION OF THE DRAWINGS The invention is explained more in detail below with reference to the drawings, in which Fig 1 is a graphical presentation of a comparison of a gel according to the invention and a commercial gel with respect to exchange of fluid in contact with agar, Fig 2 is a graphical presentation of a comparison of a gel according to the invention and a commercial gel with respect to exchange of fluid in contact with gelatine, Fig 3 is a diagram showing interference of fibroblast chemotaxis of a hydrogel according to the invention on fibroblasts in vitro, Fig 4 is a diagram showing interference of keratinocyte chemotaxis of a hydrogel according to the invention on keratinocytes in vitro, Fig 5 is a photo showing a wound on a pig treated with the gel according to the invention after 24 hours, Fig 6 is a photo showing a wound on a pig treated with a known gei after 24 hours, Fig 7 is a photo showing a wound on a pig treated with the control after 24 hours, and Fig 8 is a diagram showing the effect of hydrogels on the proliferation of keratinocytes DETAILED DESCRIPTION OF THE INVENTION The invention relates to an amorphous hydrocoUoid gel composition which comprises a a water insoluble, water swellable cross-linked cellulose derivative b an alginate, and c distilled water in a sufficient amount to make up the difference between the amount of ingredients a + b and 100% and wherein the gel comprises from 0 05 to 5 00% by weight of alginate The alginate may be an alkali metal or alkaline earth metal alginate such as sodi- urn alginate or potassium alginate or calcium alginate, preferably calcium alginate being insoluble in water A water insoluble, water swellable cross-linked cellulose derivative is preferably cross-linked sodium carboxymethylcellulose, CMC The cellulose derivative is preferably used in the form of a powder It has surprisingly been found that a hydrocoUoid gel of the invention compπsing an alginate and a water insoluble, water swellable cross-linked cellulose derivative shows excellent debridmg and superior absorption properties and speeds up debπdement of necrotic tissue in chronic wounds and speeds up the healing of the wound The addition of potential antigenic components is minimised in the hydrocoUoid gei according to the invention Thus, the addition of propylene glycol being present in the formulation disclosed in EP 0 567 311 A2, or the addition of a polyol in the form of a dihydroxy alkane as disclosed in EP 0 576 523 B1 which may give concern due to the toxicity and/or antigenicity of these compounds is not necessary according to the present invention The addition of mineral oil as disclosed in EP 0 512 855 A2 may also be avoided according to the present invention Furthermore, it has been found that the formulation according to the invention shows a better performance in that it gives rise to a better absorption capacity and the same ability of debridmg and a more rapid healing than the known hydro- colloid wound gels Thus, the hydrocoUoid wound gel according to the invention differs from the wound hydrating gel disclosed in WO 95/17166 in that the gel of the invention comprises a cross-linked cellulose derivative and an alginate wherein the amount of cellulose derivative is greater than the amount of alginate It has surprisingly been found that the gel of the present invention is superior as compared with the wound hydrating gel disclosed in WO 95/17166 as it shows a much higher absorption of fluid in a moist environment It is preferred that the hydrocoUoid gel composition of the invention comprises from 0 05 to 25.00% by weight of water insoluble, water swellable cross-linked cellulose derivative, more preferred from 0 1 to 15% by weight of water insoluble, water swellable cross-linked cellulose derivative and preferably from 1 to 5% by weight of water insoluble, water swellable cross-linked cellulose derivative The hydrocoUoid gel composition of the invention preferably comprises from 0 05 to 5 00% by weight of alginate The viscosity of a hydrogel of the invention for debπdement of wounds will advantageously be in the range from about 450,000 cPs to about 2,500 000 cPs and will preferably be in the range of 500,000 to about 2,000,000 cPs when determined using a Brookfield He path Spindle T-F, 2 5 rp at ambient temperature The viscosity of a hydrogel of the invention for cell transplantation purposes will advantageously be in the range from about 1 ,500 to about 300,000, preferably from about 2,000 to about 200,000 cPs when determined using a Brookfield Hehpath Spindle T-F, 2 5 rpm at ambient temperature The hydrocoUoid gel of the invention may comprise wound healing associated in- dιcator(s), cushions or similar device for treatment or prophylaxis of formation of wounds and/or skin abnormalities This opens for a concomitant medical treatment of the wound and an easy and non-contaminating application of the active ingredients, e g. by incorporating active ingredients such as a cytochine such as growth hormone or a polypeptide growth factor giving rise to the incorporation of such active substances in a form being apt to local application in a wound in which the medicament may exercise its effect on the wound, other medicaments such as bacteπostatic or bactericidal compounds, e g iodine, lodopovidone complexes, chloram e, chlorohexidine, silver salts such as sulphadiazme, silver nitrate, silver acetate, silver lactate, silver sulphate or silver chloride , zinc or salts thereof, metronidazol, sulpha drugs, and penicillins, tissue-healing enhancing agents, e g RGD tπpeptides and the like, proteins, ammo acids such as tauπne, vitamins such ascorbic acid, enzymes for cleansing of wounds, e g pepsin, tryp- sm and the like, proteinase inhibitors or metalloproteinase inhibitors such as II- lostat or ethylene diamine tetraacetic acid, cytotoxic agents and proliferation inhibitors for use in for example surgical insertion of the product in cancer tissue and/or other therapeutic agents which optionally may be used for topical applica- tion, pain relieving agents such as lidocaine or chmchocaine, emollients, retinoids or agents having a cooling effect which is also considered an aspect of the invention In accordance with another aspect of the invention the hydrocoUoid gel composition comprises an enzyme enhancing the debπdement of wounds Preferably, the enzyme is Kπll enzyme In the present context growth hormone is intended to designate any growth hormone which is applicable in accordance with the invention such as human, bovine, ovine, porcine, equine, salmon or tuna growth hormone or analogues or derivatives thereof such as shortened or extended growth hormones such as methionyl growth hormone A growth hormone is preferably human growth hormone Wound healing associated ιndιcator(s) may e.g be indicators of pH, partial pressure of O 2 , temperature, radical mechanisms or biotechnological assays, e g indicating formation and/or degradation of collagen The hydrogel may be applied directly into the wound to be treated and is then suitably covered by a cover dressing having a suitable moisture vapour transmission rate to avoid a drying of the wound in order to ensure a moist wound healing. One suitable cover dressing for a hydrogel according to the invention is sold under the trademark Tegaderm®. Other suitable cover dressings are e.g Comfeel® Plus Ulcer Dressing or Comfeel® Plus Transparent Dressing. The hydrogel of the invention may be applied in a gauze pad or a pad of a non- woven or woven material or in an open cell foam dressing. In another aspect the invention relates to a method for preparing a hydrocoUoid gel formulation wherein a cross-linked, water insoluble, water swellable cross-linked cellulose derivative , preferably carboxymethylcellulose derivative, is mixed with water and an alginate powder. The mixing may be carried out using a dissolver or another suitable mixer which enables the admixture of the water and formation of the hydrogel. In a further aspect the invention relates to a wound dressing comprising a hydro- colloid gel containing a water insoluble, water swellable cross-linked cellulose derivative, an alginate; and distilled water in a sufficient amount to make up the difference between the amount of the other ingredients and 100% and wherein the gel comprises from 0.05 to 5.00% by weight of alginate. Further, the invention relates to a wound dressing comprising a gel containing a water insoluble, water swellable cross-linked cellulose derivative, an alginate, and distilled water in a sufficient amount to make up the difference between the amount of the other ingredients and 100% and wherein the gel comprises from 0.05 to 5.00% by weight of alginate. The hydrogel of the wound dressing of the invention preferably comprises from 2.00 to 8,00% by weight of cross-linked sodium carboxymethylcellulose, more preferred from 3 to 5% by weight of water insoluble, water swellable cross-linked cellulose derivative The contents of an alginate in the hydrogel in a dressing of the invention is preferably from 0 5 to 1 00%, more preferred 0 1 - 2%, by weight of alginate prefe- rably calcium alginate A dressing according to the invention comprising a hydrocoUoid gel containing a water insoluble, water swellable cross-linked cellulose derivative, an alginate, and distilled water in a sufficient amount to make up the difference between the amount of the other ingredients and 100%, wherein the gel compπses from 0 05 to 5 00% by weight of alginate may e g be prepared in an analogous manner as disclosed in WO 94/15562 In a further aspect the invention relates to a vehicle for use for transplantation of cells to a wound, said vehicle comprising an amorphous hydrocoUoid gel composition which comprises a a water insoluble, water swellable cross-linked cellulose derivative b an alginate, and c distilled water in a sufficient amount to make up the difference between the amount of ingredients a + b and 100% and wherein the gel comprises from 0 05 to 5 00% by weight of alginate In yet a further aspect, the invention relates to the use of an amorphous hydro- colloid gel composition which comprises a a water insoluble, water swellable cross-linked cellulose derivative b an alginate, and c distilled water in a sufficient amount to make up the difference between the amount of ingredients a + b and 100% and wherein the gel comprises from 0 05 to 5 00% by weight of alginate for debπdement of necrotic tissue in a wound and for speeding up the healing of the wound Still further, the invention relates to the use of an amorphous hydrocoUoid gel composition which comprises a. a water insoluble, water swellable cross-linked cellulose derivative b. an alginate; and c. distilled water m a sufficient amount to make up the difference between the amount of ingredients a. + b. and 100% and wherein the gel comprises from 0.05 to 5.00% by weight of alginate as vehicle for transplantation of cells to a wound The invention is illustrated more in detail in the below examples which are provi- ded to illustrate presently contemplated preferred embodiments and the best mode for practising the invention, but are not intended to be limiting thereof EXPERIMENTAL PART MATERIALS AND METHODS SMTL test method TM-70 (Surgical Materials Testing Lab., "Fluid Exchange in Hydrogel Dressings"). Used to determine the ability of a hydrogel to absorb liquid from or donate liquid to a semisohd substance. Determination of epithelialization was carried out as described by Agren M. (Br J Plast Surg 1996;49: 129-134) Determination of viscosity was carried out using a Brookfield, Helipath Spindle T-F, 2.5 rpm, ambient temperature Carboxymethylcellulose (CMC): Cross-linked CMC (AQUASORB® A500) from Aqualon, a Division of Hercules Incorporated. Calcium Alginate: Grindsted Alginate PH 470-S2 from Danisco Ingredients Agar from Oxoid. Gelatin from Sigma CaCI 2 from Struers, NaCI from Struers Mixer Dissolver APV 60S from Gπeser. Syringes 60 ml OMNIFIX® from BRAUN Serum-free medium: KGM from Clonetics Ultrapure sterile water: Maxima from Elga PBS (Phosphate buffered saline) 120 mmol/l NaCI, 2.7 mmol/l KCI, 10 mmol/l phosphate from Sigma Human skin fibroblasts: primary cultures of normal human dermis 3% H 2 O 2 gel: BRINT OVER ILTE GEL from Danapharm. EXAMPLE 1 Preparation of an amorphous hydrogel according to the invention Hydrogels were prepared as follows: The cross-linked Carboxymethylcellulose and the calcium alginate powders were mixed in a dissolver mixer at setting 0/500 rpm corresponding to low speed using two dissolver disks. Two thirds of the calculated amount of distilled water was added, and the speed was increased to 30/2000 rpm and mixing was performed for 7 minutes. The remainder of the water was added over a period of about 5 minutes, and the speed was further in- creased to 60/3000 rpm and the pressure was reduced to vacuum (water pump) for 10 minutes. The resulting gel was filled into disposable syringes which were stoppered and packed in peel-bags which were welded and sterilised in an autoclave at 120 degrees C for 20 minutes plus equalisation Gels having the compositions were produced shown in the below Table 1 Table 1 Composition of gels according to the invention Gel/ A B Constituent % grams % grams Cross-linked 3.31 993 4.80 1440 CMC Calcium 0.37 11 1 0.53 159 alginate Distilled water 96.32 28896 94.67 28401 Total 100 30000 00 30000 The viscosity of the gels according to the invention as well as the viscosity of IntraSite® Gel was determined using a Brookfield viscometer and the results appear from the below Table 2. Table 2 Viscosity of gels according to the invention and of IntraSite® Gel Gel Viscosity in cPs Hydrogel A 800000 Hydrogel B 1600000 IntraSite® Gel 400000 EXAMPLE 2 Absorbing capacity of a hydrogel according to the invention as compared to the absorbing capacity of a commercial hydrogel Method: An amorphous hydrogel according to the invention was compared with a commercial hydrogel sold as IntraSite® Gel by Smith & Nephew with respect to its ability to absorb liquid from or donate liquid to a semisohd substance 5.0 ± 0.2 grams samples of the test material were placed inside a series of plastic chambers each resting upon a piece of nylon net having a pore size of 100 microns. The chambers were weighed and placed on the surface of a series of agar and gelatine gels of varying strength made up with a solution containing 142 mmol sodium ions and 2.5 mmol of calcium ions per litre. After 48 hours the test chambers were removed from the gels, reweighed and the absolute and the percentage change of each sample calculated. The results of the test to determine the fluid affinity of the two hydrogels are summarised in Tables 3 and 4 below and expressed graphically in Figures 1 and 2. Results The results clearly show that the hydrogel according to the invention is superior as compared to the commercial IntraSite® Gel with respect to absorption of fluid in a moist environment (Table 3) and similar donation of fluid in a dry environ- ment (Table 4) This is critical in the moist part of the debπdement of a wound during which the gel shall have as high absorption capacity as possible to avoid leakage and maceration of the peπulcer skin and still retain a high viscosity in order to remain in the wound cavity Donation is critical when moisturising the dry necrotic tissue Table 3 Fluid affinity of hydrogel of invention and IntraSite® Gel against agar (Absorption) Dressing Concentration of Absolute Percentage inagar increase in crease in weight (% w/w) weight (%) (g) Amorphous Hy1 2 99(0,10) 59 74(1 92) drogel of the 2 2 49(0 1 1 ) 49 89(2 13) invention 4 1 58(0 20) 31 52(3 90) 6 0 94(0 09) 18 73(1 86) 8 0 63(0 13) 12 70(2 51 ) IntraSite® Gel 1 1 51(0 13) 30 23(2 58) 2 1 38(0 06) 27 54(1 17) 4 0 60(0 06) 12 04(1 24) 6 0 43(0 13) 8 69(2 55) 8 0 30(0 13) 5 93(2 52) Figures in parentheses designates standard deviation (SD) Table 4 Fluid affinity of hydrogel of invention and IntraSite® Gel against gelatine (Donation) Dressing Concentration of Absolute Percentage degelatine decrease in crease in weight (% w/w) weight in gram Amorphous Hy20 0.07(0.01 ) 1.41 (0.21) drogel of 25 0.30(0.01 ) 5.92(0.16) invention 30 0.49(0.04) 9.72(0.70) 35 0.65(0.04) 12.94(0.82) IntraSite® Gel 20 0.00(0.03) 0 00(0.67) 25 0.28(0.02) 5 53(0.50) 30 0.48(0 12) 9.51 (2.48) 35 0.61 (0.08) 12.25(1.67) Figures in parentheses designates standard deviation (SD) EXAMPLE 3 In vitro cohesive strength of hydrogel according to the invention and a known gel Method: The cohesive strength of a hydrogel is decisive for its suitability for use in an exuding wound. Good cohesion is necessary in order that the gel may remain on the wound site, also after having absorbed wound exudate, preventing a spreading of wound exudate which would hamper the penulcer skin and thereby the healing. The cohesive strength of the hydrogels was determined as published by poster presentation In-Vitro Cohesive Properties of Wound Gels at the fifth European Conference in Wound Management, Harrogate, 21-24 November 1995 for undiluted gels and for gels diluted with two parts isotonic saline to simulate a situation wherein the gel has absorbed wound fluid. Six grams of gel was placed on a test cell comprising two opposite plates having an area of 1256 cm2 Firstly, the gels were compressed using a mass of 2.3 kg for 30 seconds , excess gel was remo- ved from the sides of the test cell, and the peak force needed to separate cohesively a sample of gel was measured. The cohesive strength of a gel according to the invention prepared as described in Example 1 a was compared to the cohesive strength of a commercial gel, IntraSite® Gel, and the results are presented in the below Table 5 Table 5 Cohesion in N Gel Invention IntraSite® Gei Invention IntraSite® Gel Diluted to Dilution Undiluted Undiluted 33% Diluted to 33% Sample No 1 5 871 5 104 3 368 1 93 2 6 439 4 986 3 284 1 938 3 6 176 4 875 3 368 1 801 4 6 123 4 963 3 197 1 77 5 6 317 5 062 3 315 1 759 Mean 6 19 5 00 3 31 1 84 SD 0 21 0 09 0 07 0 09 SD designates Standard Deviation It appears that the gel according to the invention has a higher cohesion than the commercial gel in an undiluted state and that the cohesion of the gel of the invention is clearly higher when diluted to 33% indication a far better ability to re- main in the wound when applied to an exuding wound EXAMPLE 4 Inhibition of chemotaxis of a hydrogel according to the invention A hydrogel of the invention comprising cross-linked CMC and calcium alginate in a proportion of 4 1 and a content of CMC and alginate of in total 5 33% was pre- pared using the method as disclosed in Example 1 B The chemotaxis (migration) of human skin fibroblasts and keratinocytes in vitro was measured in order to evaluate the effect of hydrogel comprising cross-linked CMC + calcium alginate Method 5 Chemotaxis of human skin fibroblasts and keratinocytes was induced by porcine wound fluid in the lower well of modified Boyden chamber Wound fluid, wound fluid with hydrogel (0 5% w/v) and hydrogel alone were incubated for two hours at 37°C prior to the initiation of the chemotaxtic assay Suspended cells were then added to the upper well and the chambers were incubated for four hours at 0 37°C, 5% CO 2 /aιr The number of cells that had migrated through the Nuclepo- re® membrane (8 μm pores) were fixed and stained the stain was extracted with 0 1 N HCI, and the O D was measured at 620 nm Results Porcine wound fluid induced a significant chemotactic (p<0 05) response in both 15 fibroblasts and keratinocytes The chemotactic molecule for fibroblast was most likely PDGF whereas that of the keratinocytes still are unknown as EGF (50 ng/ml), IL-1α (100ng/ml) and KGF (100 ng/ml) all failed to induce chemotaxis in the keratinocytes Hydrogel inhibited chemotaxis significantly (p<0 05) in both systems 20 Conclusion The observed inhibition of chemotaxis in vitro of fibroblasts and keratinocytes by hydrogel components may indicate that the chemotactic molecules are sequestered by the hydrogel components In a wound, a hydrogel according to the invention thus may function as a reservoir for biologically active molecules which 25 are released at a rate optimal for cellular migration The results are shown in Figures 3 and 4 showing the determinations of O D for the hydrogel of the invention , hydrogel and wound fluid (WF) and wound fluid alone EXAMPLE 5 Effect of a hydrogel according to the invention on keratinocyte proliferation as compared to known hydrogels Method Confluent human keratinocytes grown in serum-free medium were exposed to different hydrogels (Gel according to invention, IntraSite® Gel, 3% H 2 0 2 -gel, pro- pylene glycol) at final concentrations of 0 2%, 2% and 10% for 24 hours Dilutions were carried out using KGM + 10% ultrapure, sterile water Control cells were treated with KGM + 10% ultrapure, sterile water (final concentration) Proliferation was assessed using incorporation of the thymidme analogue bromodeo- xyuπdine at 10 μM (BrdU) during the 24 hour-incubation period Incorporated BrdU was immunodetected using an ELISA-kit from Boehπnger Mannheim (Cat No 1 647 229) Results An inhibition of keratinocyte proliferation was found with all treatments and dosages However, at the highest concentration (10%) the inhibition was significantly more pronounced IntraSite® Gel and propylene glycol (273 mM) than for the gel according to the invention as can be seen from Fig 8 The H 2 0 2 -gel inhibited keratinocyte proliferation nearly 100% at all dosages EXAMPLE 6 The osmolality of the gels were also determined by freeze point depression and the results are presented in the below Table 6 PBS was used as control Table 6 Osmolality of gel according to invention as compared to known gels Gel Osmolality H 2 O 2 gel 2.390 osmol/kg 2 314 osmol/kg Gel of invention 0 110 osmol/kg 0 109 osmol/kg IntraSite® Gel 3 168 osmol/kg 3 164 osmol/kg 3 248 osmol/kg ) 3 132 osmol/kg PBS (Control) 0 290 osmol/kg 0 289 osmol/kg The results show that the gel according to the invention is more suitable for use for wound healing and especially for transplantation of cells to wounds in order to speed up re-epithelialization as the osmolality of the gel of the invention is more similar to the isotonic and physiological osmolality which is represented by the control EXAMPLE 7 Effect on wound healing of a hydrogel according to the invention as compared to the effect of healing of a commercial hydrogel The effect on healing (re-epithelialization) in the treatment of partial thickness wounds in pigs was evaluated by a comparative study Method The experiments were carried out as described in M Agren 1996 Partial thick- ness wounds (2 5x2 5x0 04 cm) were made in each of six domestic pigs (60±6 kg) The treatments were allocated with two regions on each pig in a randomised manner The first region comprised the four most cephalad wounds and the second region the most caudal wounds The wounds were each treated with 1 5 - 2 0 ml of gel administered from 5 ml syringes and covered with Tegaderm® Dressing. Tegaderm® Dressing alone was used as control On the 24th postoperative hour, the dressings were removed, the wounds were cleansed with saline, a fresh portion of gel was administered, and the wounds were again covered with Tegaderm® Dressing. The animals were killed after 66 hours and the wound sites excised and fixed using formalin Four sections spaced 4 - 5 mm from each other from each wound were stained using Hematoxyliπ-eosin and assessed for epithelium coverage (expressed as a percentage) by light microscopy by a "blinded" investigator. The hydrogel of the invention was produced using the method as described in Example 1 and had the following composition" 4 8% (w/v) Cross-linked CMC, 0 53% (w/v) calcium alginate and 94.67% water As comparison IntraSite® Gel from Smith & Nephew was used together with 3% hydrogen peroxide in poloxamer 407 Results Minor displacements (2-3 mm) of the secondary dressing was observed in one or two wounds in each animal after 24 and 66 hours The gel according to the invention remained macroscopically intact as opposed to the other gels which had disappeared at both time points as appears from Figs. 5 - 7 being photos of he wound sites after 66 hours The results are summarised in the below Table 7 Morphometπc evaluation followed by analysis of variance revealed statistically significant (p<0.05) differences between the treatments Table 7 Re-epithelialization of partial thickness wounds in pigs Conclusions The gel of the invention resulted in significantly higher epithelial coverage than the other treatments and the gel according to the invention accelerates epithelialization of clean superficial wounds by more than 20% as compared to the control EXAMPLE 8 Hydrogel according to the invention as vehicle for cells Method Hydrogel diluted 4 times with phosphate buffered saline (PBS) was used for these experiments (1 part gel 3 parts PBS) Cells (human skin fibroblasts) were suspended in 0 4 ml diluted hydrogel or 0 4 ml of PBS alone to a concentration of 5 x 10 5 cells/ml and added to incubation wells (surface area. 2 cm 2 ) coated with monomenc type I bovine collagen, fibnllar type I bovine collagen or plastic alone (polystyrene) The cells were incubated for 4 hours at 37 °C in 5% C0 2 and then 1 6ml culture medium (DMEM + 10% fetal calf serum)/well was added The 24-well plate was incubated for a further 72 hours The medium was removed, and the cells washed with PBS, fixed and stained with Diff-Quik The wells were photographed and slides evaluated Results Fibroblasts suspended in a gel according to the invention (diluted) migrated to an artificial wound surface (collagen) to the same extent as from PBS as shown from the below Table 8 Table 8 Adherent fibroblasts in PBS or in gel according to the invention after 3 days of incubation Treatment Surface PBS Gel of invention Polystyrene + ' +++++ Monomenc collagen ++* +++ Ftbrillar collagen +++ +++ + = few fibroblast, +++ = many fibroblasts, +++++ = abundant of fibroblasts * = abnormal morphology of fibroblast Conclusions This in vitro study clearly indicates that a gel according to the invention is an appropriate vehicle for fibroblasts to be transplanted to a wound Furthermore, the gel of the invention also appears to have a beneficial and stabilising effect on cells under stress EXAMPLE 9 Absorbing capacity of a hydrogel according to the invention as compared to the absorbing capacity of a known hydrogel . Method An amorphous hydrogel according to the invention was compared with a gel known from WO 95/17166 with respect to its ability to absorb liquid from or donate liquid to a semisolid substance by the method disclosed in Example 2 using 2% Agar and 35% gelatine The results of the test to determine the fluid affinity of the two hydrogels are summarised in Tables 9 and 10 below Results. The results clearly show that the hydrogel according to the invention is superior as compared to gel known from WO 95/17166 with respect to absorption of fluid in a moist environment (Table 9) and similar with respect to donation of fluid in a dry environment (Table 10) This is critical in the moist part of wound to be debπ- ded in which the gel shall have as high absorption capacity as possible to avoid leakage and maceration of the peπulcer skin Table 9 Fluid affinity of hydrogel of invention and gel known from WO 95/17166 against agar (Absorption) Dressing Concentration of Absolute Percentage inagar increase in crease in weight (% w/w) weight (%) (g) Amorphous Hy2 2 40 24 00(0 26) drogel of the invention gel known from 0 75 WO 95/17166 2 7 52(0 06) Figures in parentheses designates standard deviation (SD) Table 10 Fluid affinity of hydrogel of invention and gel known from WO 95/17166 against gelatine (Donation) Dressing Concentration of Absolute Percentage degelatine decrease in crease in weight (% w/w) weight in gram Amorphous Hy35 1 07 10 68(0 20) drogel of invention Gel known from 35 1 12 1 1 18(0 22) WO 95/17166 Figures in parentheses designates standard deviation (SD) EXAMPLE 10 In vivo comparison of a hydrogel according to the invention and IntraSite® Gel with respect to debridmg effect, wear time and pain and ease of use 32 patients having a leg ulcer were treated with a hydrogel according to the invention or IntraSite® Gel covered with a hydrocoUoid dressing (COMFEEL+ Dres- sing) or a film dressing (OpSite® Flexigπd) according to the evaluation by the physician Inclusion criteria were patients with a clinically non-infected leg ulcer covered by necrosis and/or fibrin, patients more than 18 years of age Exclusion criteria Erysipelas or other symptoms of clinical wound infections, acti- ve vasculitis, disease requiring systemic treatment with corticostreoids or other immunosuppressants, pregnancy and breast feeding The patients were randomised and treated for a period of 2 - 4 weeks and the wounds were evaluated weekly The weekly evaluation comprised Area covered by necrosis/fibrin, appearance of surrounding skin, dressing performance, pain experience during change of dressing, wear time, and photography Two patients treated with IntraSite® Gel dropped out due to an allergic reaction to the product There was a significant difference in the reduction of area covered with fibπn/ne- crosis In the group treated with the hydrogel of the invention, the area was reduced during the first two weeks, whereas the area was almost constant during the first two weeks in the group treated with IntraSite® Gel whereafter a reduction of the area was seen No difference was detected between the groups with respect to appearance of surrounding skin With respect to ease of application, there was a significant difference in favour of the gel of the invention partly due to a high cohesion of the product and reduced tendency of flowing during application as compared to IntraSite® Gel The group treated with the hydrogel of the invention experienced a significantly lower pain during treatment than the group treated with IntraSite® Gel The median score of wear time was four days in the group treated with the hydrogel of the invention and three days in the group treated with IntraSite® Gel These results indicates a faster initiation of debπdement when using the hydrogel of the invention and also a longer wear time

Claims: CLAIMS 1 A hydrocoUoid gel composition, especially a wound gel composition, which comprises a a water insoluble, water swellable cross-linked cellulose derivative 5 b an alginate, and c distilled water in a sufficient amount to make up the difference between the amount of ingredients a + b and 100% and wherein the gel comprises from 0 05 to 5 00% by weight of alginate 2 A hydrocoUoid gel composition as claimed in claim 1 which comprises from 10 2 00 to 8 00% by weight of cross-linked water swellable cellulose derivative, 3 A hydrocoUoid gel composition as claimed in claim 2 which comprises from 3 to 5% by weight of cross-linked water-swellable cellulose derivative 4 A hydrocoUoid gel composition as claimed in any of claims 1 - 3 which comprises from 0 5 to 1 00% by weight of alginate 15 5 A hydrocoUoid gel composition as claimed in any of claims 1 - 4 which further comprises an active ingredient 6 A method for preparing a hydrocoUoid gel formulation wherein a cross-linked water-swellable cellulose derivative powder is mixed dry with alginate powder and then water is admixed 20 7 A method as claimed in claim 6 wherein the mixing is carried out using a dissolver 8 A wound dressing comprising a gel containing a water insoluble, water swellable cross-linked cellulose derivative, an alginate, and distilled water in a sufficient amount to make up the difference between the amount of the other ingredients and 100% and wherein the gel comprises from 0 05 to 5 00% by weight of alginate 9 Use of an amorphous hydrocoUoid gel composition which comprises a a water insoluble, water swellable cross-linked cellulose derivative 5 b an alginate, and c distilled water in a sufficient amount to make up the difference between the amount of ingredients a + b and 100% and wherein the gel comprises from 0 05 to 5 00% by weight of alginate for debπdement of necrotic tissue in a wound and for speeding up the healing of the wound 10 10 Use of an amorphous hydrocoUoid gel composition which comprises a a water insoluble, water swellable cross-linked cellulose derivative b an alginate, and c distilled water in a sufficient amount to make up the difference between the amount of ingredients a + b and 100% and wherein the gel comprises from 5 0 05 to 5 00% by weight of alginate as vehicle for transplantation of cells to a wound

Inventors: AAGREN SVEN PER MAGNUS, NIELSEN PETER SYLVEST, WULFF TRINE, AAGREN, SVEN, PER, MAGNUS, NIELSEN, PETER, SYLVEST, WULFF, TRINE
Applicants: AAGREN SVEN PER MAGNUS, COLOPLAST AS, NIELSEN PETER SYLVEST, WULFF TRINE, AAGREN, SVEN, PER, MAGNUS, COLOPLAST A/S, NIELSEN, PETER, SYLVEST, WULFF, TRINE
Application year: 1997
Main classification: A61L15
IPC codes: A61K47, A61L15, A61L26, C08L1