A method for the activation of blood coagulation factor XII

Abstract: The present invention relates to a novel method for the activation of a blood coagulation factor XII using a receptor for globular heads of C1q, one of complement components. Furthermore, use of a receptor for globular heads of C1q in biological assays containing blood coagulation factor XII is also described.

Description: The present invention relates to a novel method for the activation of a blood coagulation factor XII using a receptor for globular heads of Clq, one of complement components. Technical Background A plasma kallikrein-kinin system is a series of reaction systems in vivo producing kinins which have various physiological activities. The plasma kallikrein-kinin system is deeply involved in controlling the functions of living organisms having a close relation with other various enzymatic reaction systems such as renin-angiotensin system, blood coagulating system, fibrinolysis system, complement system and arachidonate cascade mainly for prostaglandin, leukotriene and thromboxane or catecholamine. Kinins, such as bradykinin, which are the products of kallikrein-kinin system have various physiological activities such as control of hypertension by dilation of peripheral blood vessels, increase in permeability of blood vessels, contraction and relaxation of smooth muscles, generation of pain, migration of leukocytes and liberation of catecholamine from adrenal cortex and, in addition, they are known as mediators for inflammation reactions including allergic reaction whereby their significance in living organisms is great. Accordingly, it is believed that the substances which suppress the actions of kinins or inhibit the production thereof are useful as pharmaceuticals such as anti-inflammatory agents, analgesic agents and antiallergic agents. An initial stage in the series of reaction systems of the plasma kallikrein-kinin system is activation of a blood coagulation factor XII (FXII). When FXII is activated in vivo by injury or by an invading stimulation to tissues or the like, a series of enzymatic reactions of plasma kallikrein-kinin system proceeds. Thus, the activated FXII (blood coagulation factor XII of an activated form; FXIIa) acts on the plasma prekallikrein existing in the same plasma converting it to plasma kallikrein which is an enzyme of an activated form, and then, the resulting plasma kallikrein acts on a high molecular weight kininogen (HMWK) in the plasma whereupon bradykinin having various physiological activities as mentioned above is produced and liberated. The activating reaction of FXII plays very important roles not only as a reaction for initiating the plasma kallikrein-kinin system but also as a reaction for controlling the initiating steps of various other function-controlling systems in living organisms such as the blood coagulating system, fibrinolysis system, renin-angiotensin system, complement system and arachidonate cascade. FXII has been known to be activated on a heterologous surface having negative charge and examples of such a heterologous surface are kaolin, glass, Celite, dextran sulfate, collagen and acidic mucopolysaccharides although substances which activate FXII in actual living organisms have not been identified yet. Detailed Description of the Invention An object of the present invention is to offer a novel FXII-activating method which is different from the conventional method using a heterologous surface having negative charge and wherein a FXII-activating substance in living organisms is identified for a more faithful reproduction of actual FXII-activating reaction in living organisms. The present inventors have conducted an intensive study for an FXII-activating substance existing in living organisms and have found as a result thereof that a receptor protein for globular heads of Clq is a complement component existing on the cell membrane is a substance which activates the FXII whereupon the present invention has been accomplished. The present invention relates to a method for the activation of FXII using a receptor for globular heads of Clq (hereinafter, referred to as gClqR) and also to an activator for FXII consisting of said substance. "Complement" is a general name for about twenty kinds of serum protein and plays an important role as a mediator for various immune reactions and allergic reactions. It inherently exists in blood in an inactive form but, when activated by an antigen-antibody complex, membrane components of bacteria or animal cells, etc., it shows various biological activities such as hemolytic or bacteriolytic reaction, promotion of phagocytosis, promotion of inflammation, etc. Activation of the complement is initiated by contact with the above-mentioned activator whereby the complement component "Cl" is activated. Clq is one of the subcomponents of three kinds of Cl and, since it has an activity of binding to various cells such as lymphocytes, monocytes, macrophages, neutrophils, eosinophils, fibroblasts, platelets, endothelial cells and synovial cells, the presence of receptor on cell membranes of them has been suggested and isolated/identified. The present invention relates to a method for the activation of FXII using a receptor protein (gClqR) to the globular heads of the above-mentioned complement component "Clq". The method of the present invention is useful for giving more faithful reproduction and reflection of the actual FXII-activating reaction in living organisms. Joseph et al., PNAS, 1996, 93, 8552-8557 and Herwald et al., J. Biol. Chem., 1996, 271, 13040-13047 describe the binding, but not the activation, of FXII to gCl 2 R. The gClqR used in the present invention can be prepared by separation and purification from cells such as endothelial cells, lymphocytes, monocytes, macrophages, neutrophils, eosinophils, fibroblasts, platelets and synovial cells of various animals including human being. With respect to the cells, not only normal ones obtained from the components of living organisms but also cultured and proliferated cells or tumorigenically transformed cultured cells may be utilized. Separation from gClqR and succeeding purification may be conducted utilizing the means which have been commonly used for purification of membrane proteins. For example, a method wherein cell membrane is solubilized using detergents followed by purifying with various chromatographic means is a common one. Affinity chromatography using a carrier obtained by insolubilization of gClq, HMWK or the like is an especially effective method since it utilizes a biochemical affinity with gClqR whereby it has a highly separating ability and is capable of separating from other membrane proteins in a specific manner. Further, a recombinant gClqR which is prepared by means of gene engineering may be used in the FXII-activating method of the present invention too. In accordance with said gene engineering means, a product which is uniform and contains less contaminant proteins can be easily prepared. The recombinant gClqR may, for example, be manufactured by a method of B. Ghebrehiwet et al [J. Exp. Med., volume 179, pages 1809-1821 (1994)]. The reaction system in the FXII-activating method of the present invention may be appropriately selected depending upon the object. Thus, a reaction system using common animal plasma containing FXII, a reconstituted system wherein FXII, plasma prekallikrein and HMWK which are constituting components of plasma kallikrein-kinin system are combined, etc. may be exemplified as basic reaction systems in addition to a reaction system which substantially contains only FXII. It has been confirmed that the FXII-activating method of the present invention using gClqR is dependent on zinc and it is preferred that all of the above-mentioned reactions are conducted in the presence of zinc ion. For selecting optimum reaction conditions, additives which are commonly used in the related art such as albumin and saccharides may be appropriately added to the reaction system in addition to salts such as sodium chloride and buffers. Each of the reaction conditions for the FXII-activating method of the present invention such as concentrations of gClqR, FXII, other pure proteins, additives, etc., pH, reaction temperature and reaction time may be investigated and selected whereby the object of the operators can be duly achieved. In order to measure the degree of activation of FXII by the FXII-activating method of the present invention, it goes without saying that confirmation can be done by determining the resulting FXIIa quantitatively and said quantitative determination may be conducted by the methods which are common in the related art. Such quantitative determination methods for FXIIa have been abundantly reported in literatures already and the most preferred method for the operator may be adopted. An example of the commonly used ones for determination of FXIIa is a method where the measurement is conducted utilizing the enzymatic activity of FXIIa. That is a method where the measurement is done using a substrate to FXIIa and the method using synthetic coloring substrates such as D-Pro-Phe-Arg-pNA, D-Leu-Gly-Arg-pNA and N-Benzoyl-Ile-Glu-Gly-Arg-pNA hydrochloride and methyl ester, and synthetic fluorescent substrates such as Boc-Glu(OBz)-Gly-Arg-MCA and Boc-Gln-Gly-Arg-MCA besides natural substrates such as plasma prekallikrein, blood coagulation factor XI and plasmin is simple and convenient and is commonly and widely used. The above descriptions on the constituting components of the reaction system, the reaction conditions, the measuring methods for determining the produced FXIIa, etc. concerning the present invention are mere examples for conducting the present invention and it is to be understood that the present invention is not limited thereto. The FXII-activating method using gClqR in accordance with the present invention is able to be conducted in various experimental systems. As mentioned already, the reaction for activating the FXII plays a very important role as a reaction for controlling the initial stage of various controlling systems for functions in vivo such as plasma kallikrein-kinin system, blood coagulation system and fibrinolysis system and, therefore, many investigations for the FXII activation have been conducted already for elucidating the controlling mechanism in vivo. For example, (1) elucidation for pathological and physiological significance of the FXII-activating reaction; (2) study of intrinsic substances existing in living organisms controlling the FXII-activating reaction; and (3) various studies for participation of the FXII activation in several reactions in vivo such as kinin liberation, intrinsic blood coagulation, plasminogen activation and complement system have been conducted. Method of the present invention whereby the actual FXII-activating reaction in vivo can be reproduced and reflexed in a more faithful manner in the above-mentioned testing and experimental systems is quite useful. Development of pharmaceuticals having an inhibiting or promoting action to plasma kallikrein-kinin system, blood coagulation system, fibrinolysis system, renin-angiotensin system, complement system, arachidonate cascade, etc. in which the FXII activation participates has been conducted as well. For example, a substance which inhibits the plasma kallikrein-kinin system has an inhibitory action on the production of bradykinin which is a final product thereof and, therefore, such a substance is useful as a drug for suppressing pain, inflammation, allergy, etc. induced by bradykinin such as an analgesic agent, an anti-inflammatory agent, an antiallergic agent, etc. In a method of measuring and screening the activity of such a drug, utilization of a reaction system which is nearer the system in vivo has a higher possibility of finding preferred drugs having a suitable action mechanism. Therefore, the method of the present invention having such a characteristic feature can be utilized for measuring the activity of the above-mentioned drugs and is very highly useful. Especially in measuring the activity of a drug which inhibits the FXII activation acting to gClqR, the method of the present invention is essential. Specific examples of the method for measuring the activity of the above-mentioned drugs are (1) a method of measuring the inhibiting activity of a test substance to the production of kallikrein using animal plasma (Examined Japanese Patent Publication Hei-04/14,000); (2) a method of measuring the inhibiting and promoting activities of a test substance to the production of FXIIa using animal plasma (Laid-Open Japanese Patent Publication Hei-07/51,097); and (3) a method of measuring the inhibiting and promoting activity of a test substance to the production of FXIIa, kallikrein or bradykinin by a reconstituted plasma kallikrein-kinin system (Laid-Open Japanese Patent Publication Hei-07/51,098). Brief Description of the Drawings Fig. 1 shows a result of the investigation where FXII and gClqR were mixed and made to react and the activation of FXII by gClqR was checked with a lapse of time. Fig. 2 shows a result of the investigation where activation of FXII by gClqR was checked in a plasma kallikrein-kinin system which was reconstituted from FXII, plasma prekallikrein and HMWK. The present invention will be further illustrated by way of the following examples. It is to be understood that they are mere examples for a specific explanation of the present invention and that they do not have any limitative meaning. Example 1. Preparation of gClqR. In the following FXII activating tests, gClqR which was separated/purified from endothelial cells derived from human umbilical vein (HUVEC) or recombinant gClqR was used. The recombinant gClqR used herein is a product prepared by the above-mentioned method by B. Ghebrehiwet, et al. [J. Exp. Med., volume 179, pages 1809-1821 (1994)]. In the meanwhile, gClqR was purified from HUVEC as shown below and subjected to an FXII activating test. Solubilized HUVEC membrane protein fraction was dialyzed against a 10 mM HEPES buffer containing 159 mM of sodium chloride, 2 mM of phenylmethylsulfonyl fluoride, 1 µM of aprotinin, 1 µM of pepstatin, 0.1 % of Triton X-100 and 50 µM of zinc chloride and was added to a HMWK affinity column. After the column was well washed, it was eluted with 0.1 M glycine-HCl (pH: 2.5) and each 0.5 ml of eluates was taken out. Each of the eluates was adjusted to pH 7.0 with 1 M Tris-HCl buffer (pH: 9.0) and subjected to a dot blotting by means of protein-detecting monitor and biotinated HMWK of the eluate whereupon purified gClqR was obtained. Example 2. Test of Activation of FXII. (1) FXII (20 µg/ml), 32 µg/ml of recombinant gClqR and 0.6 mM of S-2222 (synthetic substrate for FXIIa: N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginyl p-nitroanilide hydrochloride and methyl ester) were made to react at 25°C in a HEPES buffer (consisting of 10 mM of HEPES, 137 mM of sodium chloride, 11 mM of D-glucose, 4 mM of potassium chloride, 1 mg/ml of bovine serum albumin, 1 mM of calcium chloride and 50 µM of zinc chloride; pH: 7.4). The HEPES buffer was treated with (4-amidinophenyl)methanesulfonyl fluoride (APMSF) before use and the proteases contained as contaminants in albumin was inactivated for the sake of safety. p-Nitroaniline was liberated from the synthetic substrate S-2222 by an enzymatic action of FXIIa produced by activation of FXII and, therefore, the produced amount of FXIIa can be determined by measuring the liberated amount of p-nitroaniline. p-Nitroaniline emits yellow light which can be determined by measuring an absorbance at 405 nm and, therefore, said absorbance with lapse of time was monitored using a spectrophotometer to measure the amount of FXIIa produced. An example of the results is shown in Fig. 1. Example 3. Test of Activation of FXII. (2) Recombinant gClqR of various concentrations, 1 µg/ml of FXII, 1 µg/ml of HMWK, 1 µg/ml of plasma prekallikrein and 0.6 mM of S-2302 (a synthetic substrate to plasma kallikrein: H-D-prolyl-L-phenylalanyl-L-arginyl p-nitroanilide dihydrochloride) were made to react at 25°C in the same APMSF-treated HEPES buffer as mentioned above. In this test system, activation of FXII was measured using the amount of kallikrein produced as an index. Thus, when FXII is activated and is converted to FXIIa, FXIIa acts on plasma prekallikrein and converts it to plasma kallikrein having an enzymatic activity. Therefore, when the amount of kallikrein produced there is measured, it is possible to determine how much of the FXII is activated. Amount of the produced kallikrein was determined by monitoring the emission of yellow color of p-nitroaniline liberated from the synthetic substrate S-2303 by a hydrolyzing action of kallikrein using the spectrophotometer by the same manner as mentioned above. An example of the results is shown in Fig. 2. The test system of Example 2 is a result of the measurement of activation of FXII by the reaction of gClqR with pure FXII only and, as shown in the result of Fig. 1, the coloration p-nitroaniline liberated from the synthetic substrate which is an index for the amount of FXIIa produced increased with a lapse of time whereby it is clear that gClqR has an action of activating the FXII. In the absence of zinc ion, said activation of FXII by gClqR was not noted at all. Further, said FXII-activation was inhibited in a concentration-dependent manner by a Cl inhibitor which is known as an endogenous inhibitor existing in plasma. The test system of Example 3 is a reconstituted system of a plasma kallikrein-kinin system consisting of FXII, plasma kallikrein and HMWK and is a result where the activation of FXII was measured using the produced amount of kallikrein as an index. As shown in the result of Fig. 2, gClqR activated the FXII in a concentration-dependent manner and, like the reaction system of Example 2, the activation was not noted in the absence of zinc ion. When the concentration of FXII was varied in this FXII activation reaction system where the amount of gClqR was made constant, the amount of the produced kallikrein increased dependent on amount of the used FXII. When gClqR purified from HUVEC was used in place of the recombinant gClqR, the same FXII activation was noted. Accordingly, it was suggested that the saccharized site in gClqR did not affect the activation of FXII. As mentioned herein above, it has now become clear that the receptor to the globular heads of Clq(gClqR) has an action of activating the FXII and said receptor is one of the actual FXII-activating substances in vivo. Therefore, the FXII-activating method in accordance with the present invention reproduces and reflects the actual FXII-activating reaction in vivo in a more faithful manner, and said method is different from the conventional ones using heterologous surface having negative charges but is a novel method for activating the FXII. As mentioned in detail above, the FXII-activating method of the present invention is very highly useful as a method which is capable of conducting a reaction system nearer the system in vivo in various studies concerning the FXII-activating reaction and also in the FXII-activation as well as in the method of measuring the activity and of screening the pharmaceutical agents participating in the reaction systems in vivo such as a plasma kallikrein-kinin system using said FXII-activation as an initial stage.

Claims: A method for the activation of blood coagulation factor XII using a receptor for globular heads of Clq, comprising mixing factor XII with a receptor to globular heads of Clq in the presence of zinc ions. A method for determination of activated factor XII by a receptor for globular heads of Clq, comprising the following steps: (a) mixing factor XII, a receptor for globular heads of Clq and a substrate for activated factor XII in the presence of zinc ions, and (b) detecting the presence of activated factor XII. A method according to claim 2, wherein a synthetic substrate for FXIIa is added. A method according to claim 2, wherein prekallikrein is the substrate for activated factor XII (FXIIa) and prekallikrein and HMWK are further added. Use of a receptor for globular heads of Clq and zinc ion for the activation of blood coagulation factor XII. Use of a receptor for globular heads of Clq and zinc ion in an assay to identify substances which inhibit or promote blood coagulation factor XII. Use of a receptor for globular heads of Clq in an assay comprising blood coagulation factor XII and zinc ion to identify substances which inhibit or promote the kallikrein-kinin system, blood coagulation system, fibrinolysis system, rennin-angiotensin system, complement system, or arachidonate cascade.

Inventors: KAPLAN ALLEN P, SHIBAYAMA YOJI, KAPLAN, ALLEN P., SHIBAYAMA, YOJI
Applicants: NIPPON ZOKI PHARMACEUTICAL CO, NIPPON ZOKI PHARMACEUTICAL CO., LTD.
Application year: 1997
Main classification: G01N33
IPC codes: C12Q1, C12N9, G01N33