INHIBITION OF MATRIX METALLOPROTEASES BY SUBSTITUTED PHENETHYL COMPOUNDS

Abstract: Matrix metalloprotease inhibiting compounds, pharmaceutical compositions thereof and a method of disease treatment using such compounds are presented. The compounds of the invention have generalized formula (I), wherein R<25> is a substituted phenylethyl moiety. These compounds are useful for inhibiting matrix metalloproteases and, therefore, combatting conditions to which MMP's contribute, such as osteoarthritis, rheumatoid arthritis, septic arthritis, periodontal disease, corneal ulceration, proteinuria, aneurysmal aortic disease, dystrophobic epidermolysis, bullosa, conditions leading to inflammatory responses, osteopenias mediated by MMP activity, tempero mandibular joint disease, demyelating diseases of the nervous system, tumor metastasis or degenerative cartilage loss following traumatic joint injury, and coronary thrombosis from athrosclerotic plaque rupture. The present invention also provides pharmaceutical compositions and methods for treating such conditions.

Description: INHIBITION OF MATRIX METALLOPROTEASES BY SUBSTITUTED PHENETHYL COMPOUNDS BACKGROUND OF THE rNVFNTTON Field of the Invention This invention relates to enzyme inhibitors, and more particularly, to novel substituted phenethyl compounds or derivatives thereof useful for inhibiting matrix metalloproteases. Description of the Related Art The matrix metalloproteases (a.k.a. matrix metailoendo-proteinases or MMPs) are a family of zinc endoproteinases which include, but are not limited to, interstitial collagenase (a.k.a.. MMP-1 ), stromelysin (a.k.a.. proteoglycanase, transin, or MMP-3), gelatinase A (a.k.a.. 72kDa-gelatinase or MMP-2) and gelatinase B (a.k.a.. 5kDa-gelatinase or MMP-9). These MMPs are secreted by a variety of cells including fibroblasts and chondrocytes, along with natural proteinaceous inhibitors known as TIMPs (Jissue Inhibitor of MetalloEroteinase). All of these MMPs are capable of destroying a variety of connective tissue components of articular cartilage or basement membranes. Each MMP is secreted as an inactive proenzyme which must be cleaved in a subsequent step before it is able to exert its own proteolytic activity. In addition to the matrix destroying effect, certain of these MMPs such as MMP-3 have been implemented as the in vivo activator for other MMPs such as MMP-1 and MMP-9 (Ito, et al., Arch Bio hem. Biopkys.2$ 211, 1988; Ogata, et al., J. Biol. Ch m., 2S 3581, 1992). Thus, a cascade of proteolytic activity can be initiated by an excess of MMP-3. It follows that specific MMP-3 inhibitors should limit die activity of other MMPs that are not directly inhibited by such inhibitors. SUBS It has also been reported that MMP-3 can cleave and thereby inactivate the endogenous inhibitors of other proteinases such as elastase (Winyard. et al., FEBS Letts. 279, I, 91. 1991 ) Inhibitors of MMP-3 could thus influence the activity of other destructive proteinases by modifying the el of their endogenous inhibitors. A number of diseases are thought to be mediated by excess or undesired matπx-destroying metalloprotease activity or by an imbalance in the ratio of the MMPs to the TIMPs. These include: a) osteoarthritis (Woessner, et al., J. Biol.Chem., 2J9_(6), 3633, 1984; Phadke. et al., J Rheumatoi 12, 852. 1983), b) rheumatoid arthritis (Mullins, et al., Biochim. Biophys. Λcta 6£5_, 1 17. 1983; Woolley, et al., Arthritis Rheum. 22, 1231 , 1977; Gravallese, et al.. Arthritis Rheum. 1076, 1991 ), c ) septic arthritis (Williams, et al.. Arthritis Rheum, 3 , 533, 1990), d) tumor metastasis ( Reich, et al., Cancer Res., 4& 3307, 1988, and Matrisian, et al., Proc. Nat'l. Acad. Sci., USA £3_, 9413, 1986), e) periodontal diseases (Overall, et al., J. Periodontal Res. 22, 81, 1987), f) comeal ulceration (Bums, et al., Invest. Opthalmol. Vis. Sci. 3J}, 1569, 1989), g) proteinuria (Baricos, et al.. Biochem. J. 254. 609, 1988), h) coronary thrombosis from atherosclerotic plaque rupture (Henney, et al., Proc. Nat'l. Acad. Sci.. USA SL 8154-8158, 1991), I) aneurysmai aortic disease (Vine, et al.. Gin. Sci. £L 233, 1991), j) birth control (Woessner, et al., Steroids 54, 491, 1989), k) dystrophobic epidermolysis buliosa (Kronberger, et al., J. Invest. Dermatol. 22, 208, 1982), and 1) degenerauve cartilage loss following traumatic joint injury, m) conditions leading to inflammatory responses, osteopenias mediated by MMP activity, n) tempero mandibular joint disease, o) demyelating diseases of the nervous system (Chantry, et al., J. Neurochem. ≤ , 688, 1 88). The need for new therapies is especially important in ώe case of arthritic diseases. The primary disabling effect of osteoarthritis (OA), rheumatoid arthritis (RA) and septic arthritis is the progressive loss of articular cartilage and thereby normal joint function. No marketed pharmaceutical agent is able to prevent or slow this cartilage loss, although nonsteroidal anti- iririamrnatory drugs ( SAIDs) have been given to control pain and swelling. The end result of these diseases is total loss of joint function which is only treatable by joint replacement surgery. MMP inhibitors are expected to halt or reverse the progression of cartilage loss and obviate or delay surgical intervention. Proteases are critical elements at several stages in the progression of metastatic cancer. In this process, the proteolytic degradation of structural protein in the basal membrane allows for expansion of a tumor in the primary site, evasion from this site as well as homing and invasion in distant, secondary sites. Also, tumor induced angiogenesis is required for tumor growth and is dependent on proteolytic tissue remodeling. Transfection experiments with various types of proteases have shown that the matrix metalloproteases play a dominant role in these processes in particular geiarinases A and B (MMP-2 and MMP-9, respectively). For an overview of this field see Mullins, et al., Biochim. Biophys. Acta ≤ 5_ 177, 1983; Ray, et al., Eur. Respir. J. 7, 2062, 1994; Birkedal-Hansen, et al., Crit. Rev. Oral Biol. Med. 4, 197, 1993. Furthermore, it was demonstrated that inhibition of degradation of extracellular matrix by the native matrix metalloprotease inhibitor TIMP-2 (a protein) arrests cancer growth (DeClerck, et al.. Cancer Res. 5 , 701, 1992) and that TTMP-2 inhibits tumor-induced angiogenesis in experimental systems (Moses, et al. Science 248, 1408, 1990). For a review, see DeClerck, et al., Ann. N. Y. Acad Sci. 222, 222, 1994. It was further demonstrated that the synthetic matrix metalloprotease inhibitor batimastat when given intraperitoneally inhibits human colon tumor growth and spread in an orthotopic model in nude mice (Wang, et al. Cancer Res. £4, 4726, 1994) SβδSπiϋϊESHlIT and prolongs the survival of mice bearing human ovarian carcinoma xenografts (Davies. et. al.. Cancer Res. ϋ, 2087, 1993). The use of this and related compounds has been described in Brown. et al.. WO-9321942 A2 (931 1 1 1). There are several patents and patent applications claiming the use of metaiioproteinase inhibitors for the retardation of metastatic cancer, promoting tumor regression, inhibiting cancer ceil proliferation, slowing or preventing cartilage loss associated wi osteoarthritis or for treatment of other diseases as noted above (e.g. Levy, et al., WO-9519965 Al; Beckett, et al., WO-9519956 Al ; Beckett, et al., WO-9519957 Al; Beckett et al., WO-9519961 Al ; Brown, et al., WO-9321942 A2; Crimmin, et al., WO-9421625 Al; Dickens, et al.. U.S. Pat. No. 4,599,361; Hughes, et al., U.S. Pat. No. 5.190,937; Broadhurst et al., EP 574758 Al; Broadhurst et al,. EP 276436; and Myers, et al., EP 520573 Al . The preferred compounds of these patents have peptide backbones with a zinc complexing group (hydroxamic acid, thiol, carboxylic acid or phosphinic acid) at one end and a variety of sidechains, bow those found in the natural amino acids as well as those with more novel functional groups. Such small peptides are often poorly absorbed, exhibiting low oral bioavailability. They are also subject to rapid proteolytic metabolism, thus having short half lives. As an example, batimastat, me compound described in Brown, et al., WO-9321942 A2, can only be given inrra peritoneally. Certain 3-biphenoylpτopanoic and 4-biaryioylbutanoic acids are described in the literature as anti-inflammatory, anti-platelet aggregation, anti-phlogistic, anti-proliferative, hypolipidemic, antirheumatic, analgesic, and hypocholesterolemic agents. In none of these examples is a reference made to MMP inhibition as a mechanism for the claimed therapeutic effect Certain related compounds are also used as intermediates in the preparation of liquid crystals. Specifically, Tomcufcik. et al.. US patent 3.784,701 claims certain substituted benzoylpropionic acids to treat inflammation and pain. These compounds include 3-biphenoylpropanoic acid (a.k.a. fenbufen) shown below. Fenbufen Child, et al., J Pharm. Sci., ££, 466, 1977 describes structure-activity relationships of several analogs of fenbufen. These include several compounds in which the biphenyl ring system is substituted or the propanoic acid portion is substituted with phenyl, halogen, hydroxyl or methyl, or the carboxylic acid or carbonyl functions are converted to a variety of derivatives. No compounds are described which contain a 4'-substituted biphenyl and a substituted propanoic acid portion combined in one molecule. The phenyl (compounds XLDC and LXXVII) and methyl (compound XLVLT) substituted compounds shown below were described as inactive. Kameo, et al., Chem. Pharm. Bull, 3 2050, 1988 and Tomizawa, et al., JP patent 621 2825 A2 describe certain substituted 3-biphenoylpropionic acid derivatives and analogs thereof including the following. Various compounds with other substituents on die propioruc acid portion are described, but they do not contain biphenyl residues. wherein X = H, 4'-Br, 4'-Cl, 4'-CH„ or 2 * -Br. Cousse, et al., Eur. J. Xfed Chem., 22, -*5, 1987 descπbe the following mediyl and med ylene substituted 3-biphenoyl-propanoic and -propenoic acids. The corresponding compounds in which the carbonyl is replaced with either CH,OH or CH, are also described. wherein X = H, Cl, Br, CHjO, F, or NH,. Nichl, et al. DE patent 1957750 also describes certain of the above mediylene substituted biphenoyipropanoic acids. El-Hashash, et al., Revue Roum Chim. 22, 1581, 1978 describe products derived from β-aroyl-acrylic acid epoxides including the following biphenyl compound. No compounds substituted on the biphenyl portion are described. Kitamura, et al., JP patent 60209539 describes certain biphenyl compounds used as intermediates for die production of liquid crystals including the following. The biphenyl is not substituted in these intermediates. wherein R 1 is an alkyl of 1-10 carbons. Thyes, et al., DE patent 2854475 uses d e following compound as an intermediate. The biphenyl group is not substituted. Sammour, et al., Egypt J. Chem. H, 31 1, 1972 and Couquelet et al.. Bull. Soc. Chim. Fr. 9, 3196, 1971 describe certain dialkylamino substituted biphenoylpropanoic acids including die following. In no case is die biphenyl group substituted. wherein R 1 , R 2 = alkyl, benzyl, H, or, together with die nitrogen, morphoiinyl. 7 Others have disclosed a series of btphenyi-containing carboxylic acids, illustrated by the compound shown below, which inhibit neural endopeptidase ( " NEP 24.11 ). a membrane-bound zinc metalloprotease (Stanton, et al., Bioorg. Me Chem. Lett. 4, 539, 1994; Lombaert et al.. Bioorg \(ed Chem. Lett. 4, 2715. 1994; Lombaert et al., Bioorg \fed. Chem. Lett. 5. 145. 1995; Lombaert. et al., Bioorg. Med Chem. Lett, ϊ, 151, 1995). It has been reported diat N-carboxyalkyl derivatives containing a biphenylediylglycine. illustrated by the compound shown below, are inhibitors of stromelysin-l (MMP-3), 72 kDA gelatinase (MMP-2) and collagenase (Durette, et al., WO-9529689). It would be desirable to have effective MMP inhibitors which possess improved bioavailability and biological stability relative to me peptide-based compounds of die prior art and which can be optimized for use against particular target MMPs. Such compounds are d e subject of d e present application. % The development of efficacious MMP inhibitors would afford new therapies for diseases mediated by die presence of. or an excess of MMP activity, including osteoarthritis, rheumatoid arthritis, septic arthritis, tumor metastasis, periodontal diseases, comeal ulcerations, and proteinuna. Several inhibitors of MMPs have been described in the literature, including U iols (Beszant, et al.. J Med. Chem. 3_£, 4030, 1993), hydroxamic acids (Wahl, et al. Bioorg. Med. Chem. Lett. 5_ 349, 1995; Conway, et al.J. Exp. Med. 132, 449, 1995; Porter, et al., Bioorg. Med. Chem. Lett. 4, 2741, 1994; To czuk, et al., Bioorg. Med Chem. Lett. £, 343, 1995; Castelhano, et al., Bioorg. Med. Chem. Lett. £, 1415. 1995), phosphorous-based acids (Bird, et al. J Med Chem. £7, 158, 1994; Moφhy, et al., Bioorg. Med. Chem. Lett. 4, 2747, 1994; Kortylewicz, et al., J. Med Chem. 3 , 263, 1990), and carboxylic acids (Chapman, et al. J. Med Chem. 1& 4293, 1993; Brown, et al. J. Med Chem. 37, 674, 1994; Morphy, et al., Bioorg. Med Chem. Lett. 4, 2747, 1994; Stack, et al., Arch. Biochem. Biophys. 2S2, 240, 1991; Ye, et al., J. Med Chem. 3J, 206, 1994; Grobelny, et al.. Biochemistry 24, 6145, 1985; Mookhtiar, et al., Biochemistry 22, 4299, 1988). However, these inhibitors generally contain peptidic backbones, and thus usually exhibit low oral bioactivity due to poor absorption and short half lives due to rapid proteolysis. Therefore, there remains a need for improved MMP inhibitors. SUMMARY OF THE INVENTION This invention provides compounds having rnarrix metalloprotease inhibitory activity. These compounds are useful for inhibiting matrix metalloproteases and, ti erefore, combating conditions to which MMP's contribute. Accordingly, d e present invention also provides pharmaceutical compositions and methods for treating such conditions. The compounds described relate to a method of treating a human to achieve an effect in which die effect is: alleviation of osteoarthritis. rheumatoid arthritis, septic arthritis, periodontal disease, comeal ulcerauon. protemuπa. aneurysmortic disease, dystrophic epidermolysis buliosa. conditions leading to inflammatory responses , osteopenias mediated by MMP activity, tempero mandibular joint disease, or demyelinating diseases of die nervous system; tumor metastasis and degenerative cartilage loss following traumatic joint injury; and reduction of coronary thrombosis from atherosclerotic plaque rupture. The compounds of the invention are also useful for birth control. The method according to d e invention comprises administering an amount of a compound or composition of die invention as descnbed above, and in more detail in die detailed description below, which is effective to inhibit the activity of at least one matrix metalloprotease, resulting in achievement of die desired effect. The compounds of die present invention are also useful scientific research tools for studying functions and mechanisms of action of matrix metalloproteases in botii in vivo and in vitro systems. Because of their MMP-inhibiting activity, the present compounds can be used to modulate MMP action, thereby allowing die researcher to observe die effects of reduced MMP activity in the experimental biological system under study. This invention relates to compounds having matrix metalloprotease inhibitory activity and die generalized formula: (T)-A-B-D-E-G (L) In d e above generalized formula (L), (T),A represents a substituted or unsubstituted aromatic 6-membered ring or heteroaromatic 5 • 6 membered ring containing 1 - 2 atoms of N, O, or S. T represents one or more substituent groups, d e subscript x represents d e number of such substituent groups, and A represents d e aromatic or heteroaromatic ring, designated as d e A ring /O SUB or A unit. When N is employed in conjunction widi either S or 0 in die A πng, these heteroatoms are separated by at least one carbon atom. The substituent group(s) T are independently selected from d e group consisting of halogen: alkyl; haloalkyl: alkenyl; alkynyl, benzyloxy, aikyloxy; -(CH ; ) P Q in which p is 0 or an integer of I - 4; and -alkenyl-Q in which the alkenyl moiety comprises 2 - 4 carbons. Q in d e latter two groups is selected from the group consisting of aryl, heteroaryl, -CN, -CHO, -NO : , -COR 2 , -OCOR 2 . -SOR\ -SO,R\ -CON(R 2 ),, -SO,N(R 2 ) 2 , -COR 2 , -N(R 2 ),, -N(R 2 )COR 2 , -N(R 2 )CO : R\ - l^CONOl 2 ):, -CHN 4 , -OR 4 , and -SR*. In these formulae R 2 represents H. alkyl, aryl, heteroaryl, arylalkyl, or heteroaryl-alkyl; R 3 represents alkyl, aryl, heteroaryl, arylalkyl, or heteroaryl-alkyl; and R* represents H, alkyl, aryl, heteroaryl, arylalkyl, heteroaryl-alkyl, alkenyl, alkynyl, haloalkyl, acyl, or alkyleneoxy or polyaikyleneoxy terminated widi H, alkyl, or phenyl. Unsaturation in a moiety which is attached to Q or which is part of Q is separated from any N, O, or S of Q by at least one carbon atom. The A ring may be unsubstituted or may carry up to 2 substiruents T. Accordingly, die subscript x is 0, 1, or 2. In the generalized formula (L), B represents an aromatic 6-membered ring or a heteroaromatic 5 - 6 membered ring containing 1 • 2 atoms of N, O, or S. It is referred to as the B ring or B unit. When N is employed in conjunction with either S or O in die B ring, these heteroatoms are separated by at least one carbon atom. In die generalized formula (L), D represents \ CO \ C=NOH \ C=S C x H V H / / / / OH / H '/ In the generalized formula (L), E represents a moiety of d e formula where r is 0-2, z is 1-4, Y l is H or CH 3 , and Y : is an alkyl of 3-6 carbons, a primary or secondary aminoalkyl of 3-6 carbons, a carboxylic acid of 2-5 carbons, a (l-moφholinyl)alkyl wherein the alkyl group is 0-5 carbons, an ester of 3-5 carbons, a ketone of 3-5 carbons, or a arylalkyl wherein the alkyl group is 3-5 carbons; or Y 1 and Y 2 togetiier with die nitrogen atom to which they are attached form a 1 -piperidinyl or 1-moφholinyl ring. D and G as used in the above structure represent die D and G units of d e general formula (L) and are not part of die E unit; tiiey are included merely to indicate how die D, E and G groups are linked. When r = 0 die structure above takes the form: When r -2 the alkyl moiety includes a cyclobuty ring and when r = 3 the alkyl moiety includes a cyclopentyl ring. In die generalized formula (L), G represents -POjH, -M in which M represents -COjH, -CONflt") ! or -CO j R 12 , R 12 represents alkyl of 1 - 4 carbons, and R 13 represents any of the side chains of d e 19 noncyciic naturally occurring amino acids. Pharmaceutically acceptable salts of tiiese compounds are also widiin die scope of die invention. /2- SUBSΠTUTE SHEET (RULE 2C In most related reference compounds of the prior art die biphenyl portion of the molecule is unsubstituted, and d e propanoic or butanoic acid portion is either unsubstituted or has a single methyl or phenyl group. Presence of die larger phenyl group has been reported to cause prior art compounds to be inactive as anti-inflammatory analgesic agents. See, for example. Child, et al.. J. Pharm. Sci.6& 466, 1977. By contrast it has now been found diat compounds which exhibit potent MMP inhibitory activity contain a substituent of significant size on d e propanoic or butanoic portion of die molecule. The biphenyl portions of die best MMP inhibitors also preferably contain a substituent on die 4'-position, although when die propanoic or butanoic portions are optimally substituted, die unsubstituted biphenyl compounds of die invention have sufficient activity to be considered realistic drug candidates. The foregoing merely summarizes certain aspects of die present invention and is not intended, nor should it be construed, to limit the invention in any way. All of the patents and otiier publications recited in this specification are hereby incorporated by reference in tiieir entirety. DESCRIPTION OF THE PREFERRED EMBODIMENTS This invention provides compounds having matrix metalloprotease inhibitory activity. These compounds are useful for inhibiting matrix metalloproteases and, dierefore, combating conditions to which MMP's contribute. Accordingly, d e present invention also provides pharmaceutical compositions and meώods for treating such conditions. The compounds described relate to a method of treating a human to achieve an effect in which die effect is: alleviation of osteoarthritis, rheumatoid arthritis, septic arthritis, periodontal disease, comeal uiceration, proteinuria, aneurysmortic disease, dystrophic epidermolysis buliosa, conditions leading to inflammatory responses, osteopenias mediated by MMP activity, tempero mandibular joint disease, or demyelinaung diseases of die nervous system: tumor metastasis and degenerative cartilage loss following traumatic joint injury; and reduction of coronary thrombosis from adierosclerotic plaque rupture. The compounds of the invention are also useful for birth control The method according to die ιn ennon compπses administering an amount of a compound or composition of die mvenuon as described above, and in more detail in die detailed description below, which is effective to inhibit the activity of at least one matrix metalloprotease, resulting in achievement of die desired effect. The compounds of die present invention are also useful scientific research tools for studying funcnons and mechanisms of action of matrix metalloproteases in both in vivo and m vitro systems. Because of their MMP-mhibiting activity, d e present compounds can be used to modulate MMP action, thereby allowing the researcher to observe the effects of reduced MMP acuvity in d e experimental biological system under study. More particularly, the compounds of die present invennon are materials having matrix metalloprotease inhibitory activity and die generalized formula ( " D-A-B-D-E-G (L> in which (T).A represents a substituted or unsubstituted aromauc or heteroaromaϋc moiety selected from the group consisting of: T.V r< T.V ri τ,V rϊ T . X < - / R l /t s^sπ in w ich R 1 represents H or alkyl of 1 - 3 carbons. Throughout this application, in the displayed chemical structures, an open bond indicates the point at which d e structure joins to anodier group. For example, where R 50 is is the structure O ^ O In these structures, die aromatic ring is referred to as die A ring or A unit and each T represents a substituent group, referred to as a T group or T unit Substituent groups T are independently selected from die group consisting of: die halogens -F, -Cl, -Br, and -I; alkyl of 1 - 10 carbons; haloalkyl of 1 - 10 carbons; alkenyl of 2 - 10 carbons; alkynyl of 2 - 10 carbons; benzyloxy, aikyloxy of 1-5 carbons; -{CHjJ p Q in which p is 0 or an integer 1 - 4, and -alkenyl-Q in which the alkenyl moiety comprises 2 - 4 carbons. Q in each of die latter two groups is selected from die group consisting of aryl of 6 - 10 carbons; heteroaryl comprising 4 • 9 carbons and at least one N, O, or S heteroatom, -CN, -CHO, -NO 2 , -CO,R 2 , -OCOR 2 , -SOR\ -SO,R\ -CON(R -SO j N R 2 ): , -C(O)R 2 , The groups R 2 , R\ and R 4 are defined as follows. /5 R : represents H; alkyl of 1 - 6 carbons: aryl of 6 - 10 carbons; heteroaryl comprising 4 - 9 carbons and at least one N, 0, or S heteroatom; arylalkyl in which die aryl portion contains 6 - 10 carbons and die alkyl portion contains 1 - 4 carbons; or heteroaryl-alkyl in which die heteroaryl portion comprises 4 - 9 carbons and at least one N. O, or S heteroatom and die alkyl portion contains 1 - 4 carbons. R J represents alkyl of 1 - 4 carbons; aryl of 6 - 10 carbons; heteroaryl comprising 4 - 9 carbons and at least one N, O, or S heteroatom; arylalkyl in which die aryl portion contains 6 - 10 carbons and the alkyl portion contains 1 - 4 carbons; or heteroaryl-alkyl in which die heteroaryl portion comprises 4 - 9 carbons and at least one N, O, or S heteroatom and die alkyl portion contains 1 - 4 carbons. R* represents H; alkyl of 1 - 12 carbons; aryl of 6 - 10 carbons; heteroaryl comprising 4 - 9 carbons and at least one N, O. or S heteroatom; arylalkyl in which d e aryl portion contains 6 - 10 carbons and die alkyl portion contains 1 - 4 carbons; heteroaryl-alkyl in which die heteroaryl portion comprises 4 - 9 carbons and at least one N, O, or S heteroatom and die alkyl portion contains 1 - 4 carbons; alkenyl of 2 - 12 carbons; alkynyl of 2 - 12 carbons; -(C q H^O-R 5 in which q is 1 -3, r is 1 • 3, and R 5 is H provided q is greater tiian 1 , or R } is alkyl of 1 - 4 carbons, or phenyl; -(CH 2 ),X in which s is 2 - 3 and X is halogen; or -C(O)R 2 . Any unsaturation in a moiety which is attached to Q or which is part of Q is separated from any N, O, or S of Q by at least one carbon atom, and die number of substituents, designated x, is 0. I, or 2. The substituent group T can also be an acetylene containing moiety with the general formula: ^(CH j ^CSC— lb where n is 1-4 and R 30 is selected from the group consisting of: HO-, MeO-. (n-Pr) ; N-. CH,CO : -. CH,CO : OCOj-, HO,C-, HOC-, Ph-, 3-OH-Ph-. and PhCH : 0-. provided that when R w is Ph or 3- OH-Ph. n - 0. The B ring of generalized formula (L) is a substituted or unsubstituted aromatic or heteroaromatic ring, in which any substituents are groups which do not cause d e molecule to fail to t t die active site of die target enzyme, or disrupt the relative conformations of die A and B rings, such that they would be detrimental. Such groups may be moieties such as lower alkyl, lower alkoxy, CN, NO ; , halogen, etc., but are not to be limited to such groups. In me generalized formula (L), B represents an aromatic or heteroaromatic ring selected from die group consisting of: xx -&- χ - - - in which R 1 is defined as above. These rings are referred to as die B ring or B unit rr Compounds of die general formula (L) include tiiose in which die combination of (T),-A-B. ratiier than taking one of die forms described above, has d e structure: R"-Z-CH 2 — where Z may be (CH ; )«-QH 4 -(CHj) f or (CH,) P e - 0-8, f - 0-5 and g - 0- 10. R 15 may be a straight or cyclic alkyl group of 6- 12 carbons atoms, preferably of 7- 1 1 carbon atoms, and optionally may bear one or more pharmaceutically acceptable substituents which are discussed more fully below. Any branching or substitution is preferably located at least three chain atoms away from die point of attachment of die R" group to die phenyl ring. R" may also be a polyedier of die formula R J2 O(C 2 H 4 O) h in which die subscript "h" is 1 or 2, and die group R 32 is a straight branched or cyclic alkyl group of 1-5 carbon atoms, preferably of 1 -3 carbon atoms and straight or phenyl, or benzyl. R" optionally may bear one or more pharmaceutically-acceptable substituents which are discussed more fully below. Any branching or substitution is preferably located at least tiiree chain atoms away from die point of attachment of die polyedier R 15 group to die phenyl ring. R l} may also be a substituted alkynyl group of die formula: R 33 (CH 2 j b -CsC- in which die subscript "b" is I -10 and die group R 3 is H-, HO- or R 34 ©- and die group is preferably d e HO- group. R 34 may be an alkyl group of 1-3 carbon atoms, or phenyl or benzyl. R 33 optionally may bear one or more pharmaceutically-acceptable substituents which are discussed more fully below. " may also be -H, -Cl, -OMe or R'" 0 ^ , HO , ^= It SUBSTITUTE SHEH (RULE 26) wherein n is 0-4. R' ' is C : H„ allyl or benzyl, and R !6 is one of where x is 0-4. t is 0-2, and R is one of he following: halide, alkyl of 1-6 carbons, OR, NR„ O, ( R = H or alkyl of I -6 carbons). In the generalized formula (L), D represents the moieties: \ \ \ \ .H \ .H C=0 C = OH C=S C. C / / / / OH / H In the generalized formula (L), E represents a moiety of die formula where r is 0-2, z is 1 -4, Y 1 is H or CH 3 , and Y 2 is an alkyl of 3-6 carbons, a primary or secondary aminoalkyl of 3-6 carbons, a carboxylic acid of 2-5 carbons, a (l-moφholinyl)alkyl wherein die alkyl group is 0-5 carbons, an ester of 3-5 carbons, a ketone of 3-5 carbons, or a arylalkyl wherein the alkyl group is 3-5 carbons; or Y 1 and Y 2 togedier with die nitrogen atom to which diey are attached form a 1-piperidinyl or 1-moφholinyi ring. D and G as used in die above structure represent the D and G units of die general formula (L) and are not part of he E unit; d ey are included merely to indicate how die D, E and G groups are linked. When r = 0 die structure above takes d e form: When r =2 the alkyl moiety includes a cyclobutyl ring and when r - 3 die alkyl moiery includes a cyclopentyl ring. In addition, aryl or heteroaryl portions of any of the T groups optionally may bear up to two substituents selected from the group consisting of -(CH,) v C(R")(R ι: )OH, -<CH : ) v OR". -(CH : ) v SR π . -(CH,) v S(O)R", -(CH,) y S(O),R", -(CH : ) y SO 2 N(R") : , -(CH^R 11 ),. -(CH : ) v N(R M )COR ,: . -OC(R") : 0- in which both oxygen atoms are connected to d e aryl ring, -(CH )C v OR". -<CH : ) y CON(R") : , -(CH,) y CO : R", -(CH^OCOR 11 -halogen, -CHO, -CF„ -NO,, -CN. and -R . in which y is 0 - 4; R" represents H or alkyl of 1 - 4 carbons; and R' 2 represents alkyl of 1 - 4 carbons. In the generalized formula (L), G represents -P0 3 H, -M in which M represents -CO 2 H, -CONfll"),, or -CO 2 R 12 , and R 13 represents any of die side chains of die 19 noncyclic naturally occurring amino acids. Pharmaceutically acceptable salts of the compounds falling within die generalized formula (L) are also witiύn die invention. In d e compounds of die invention, die following are preferred. The substituent group T is preferably halogen (most preferably Cl), ipt x, which defines the nr or The subscr umber of T substituents, is preferably 1 or 2, most preferably 1 , and tiiis substituent is on d e 4- position of ring A. The A ring is preferably a phenyl or thiophene ring, most preferably phenyl. The B ring is preferably a 1,4-phenylene or 2,5-thiophene ring, most preferably 1,4-phenylene. 7Ό The D unit is most preferably a carbonyi group. The E unit is preferably: where, as before. D and G are the D and G units and are not part of E, z is 1-4 (most preferably 2) and R 24 is one of die following β ^-rt The G unit is most preferably a carboxylic acid group. It is to be understood that as used herein, die term "alkyl" means straight branched, cyclic, and polycyclic materials. The term "haloalkyl" means partially or fully halogenated alkyl groups such as -(CH j ^Cl, -CFjand -C^F,,. for example. In one of its embodiments, the invention relates to compounds of generalized formula (L) in which at least one of the units A, B, T, and E comprises a heteroaromatic ring. Preferred ι heteroaromatic πng-containing compounds are ose in which the heteroaryl groups are heteroar of 4 - 9 carbons comprising a 5 - 6 membered heteroaromatic πng containing O. S, or NR 1 when the πng is 5 -membered, and N when said πng is 6-membered. Particularly preferred heteroaromatic ring-containing compounds are those in which at least one of the A and B units compπses a thiophene nng. When the A umt is duophene. it is preferably connected to B umt at position 2 and carries one substituent group T on position 5. When B Unit is duophene, it is preferably connected through positions 2 and 5 to D and A units respectively. In the generalized formula (L), d e A and B πngs are preferably phenyl and phenylene. respectively, the A ring preferably bears at least one substituent group T preferably located on the position furthest from die position of d e A πng which is connected to d e B πng, the D umt is preferably a carbonyl group, and die G umt is preferably a carboxyl group. In a particularly preferred embodiment die compounds of die invention have die formula: in which x is 1 or 2, one substituent group T is located on die 4- position of die A ring, relative to the point of attachment between die A and B rings, n ■ 1 -5 and R 24 is ortho, meu or para to die (CH 2 )„ and is selected from one of die following: Substituent group T of iis subset is preferably a halogen. T is most preferably Cl and is in the para position of die A ring relative to die B ring. The invention also relates to certain intermediates useful in die syndiesis of some of the claimed inhibitors. These intermediates are compounds having die generalized formula: where n « 1-5, R aι is a halogen and R 22 is H or an alkyl (preferably ethyl) or allylallcyl (wherein the alkyl is preferably memyl) group. >J Those skilled in the art will appreciate that many of the compounds of the invention exis t m enantiomeric or diastereomeric forms, and d at it is understood by d e art that such stereoisomers generally exhibit different activities in biological systems. This invention encompasses all possible stereoisomers which possess inhibitory activity against an MMP, regardless of their stereoisomeπc designations, as well as mixtures of stereoisomers in which at least one member possesses inhibitory activity. The most prefered compounds of die present invention are as indicated and named in the list below: D 4'<hloro-γ-oxo-α-[2-[2-[[(2-phenyledιyl)amino]carbonyl]phenyl]ethyl]-[ 1 , 1 '-biphenyl]-4- butanoic acid, IT) 4'-chloro-α-(2-[2-[[(4-morpholinylcarbonyl)phenyl]edιyl]-γ-oxo-[ 1,1 ' -biphenyl ]-4-butanoic acid, QT) 4'-chloro-γ-oxo-α-{2-{2-[[(phenylmedιyl)amino]carbonyl]phenyl]edιyl]-[ 1,1 '-biphenyl]-4- butanoic acid, rV) 4'<hloro-γ-oxo-α-[2-[2-[[(3-phenylpropyl)ammo]carbonyl]phenyl]edιyl]-[l,l'-biphenyl]-4- butanoic acid, V) 4'-chloro-γ-oxo-o-{2-[2-(l-piperidinylcarbonyl)phenyl]ediyl]-[l, -biphenyl]-4-butanoic acid, VI) 4'<hlorr «-[2-[2-[[(3-meωylbutyl)ammo]carbonyi]phenyl]ethyl]-γ-oxo-t 1,1' -biphenyl]-4- butanoic acid, VTJ) 4'-chloi O -β-{2-[2-[[(3-eώoxybutyl)amino]carbonyl]phenyl]ethyl]-γ-oxo-[ 1 , 1 '-biphenyi]-4- butanoic acid, - Vin) 4 ' -chloro-α-[2-[2-[[(2-etJιoxyedιyl ) amιno]carbonyl]phenyl]edιyl].γ-oxo-(;i.r-bιphenyl]-4- butanoic acid. DO 4'-chioro-α-[2-[2-([(2-emoxy-2-oxoethyl)medιylamιno]carbonyl]phenyl]ethyl]-γ-oxo-[ 1.1 - bιphenyl]-4-butanoιc acid, X) 4 -chloro-α-[2-[3-(4-moφholinylcarbonyl)phenyl]ethyl]-γ-oxo-[l.l '-biphenyi]-4-butanoιc acid. XI) 4' -chloro-γ-oxo-α-[2-[3-(l-piperidinylcarbonyl)phenyl]ethyl]-[l,r-bιphenyl]-4- butanoic acid, XJJ) 4'-chloro-γ-oxo-α-[2-[3-[[(2-phenylethyl)amino]carbonyl]phenyI]edιyl]-[l . -biphenyl]-4- butanoic acid, XUJ) 4'-chloro-γ-oxo- -[2-[3-{[(3-phenylpropyl)amino]carbonyi]phenyl]edιyl]-[l, -biphenyl]-4- butanoic acid, XIV) 4'-chlorcr-α-[2-[3-[[(3-memylbutyl)amino]carbonyl]phenyl]edιyl]-γ-oxo-[ 1 , 1 '-biphenyl]-4- butanoic acid, XV) 4 -chJoro-γ-oxo-α-[2-[3-[[(phenylmemyl)amino]carbonyi]phenyl]edιyl]-(l, -biphenyl]-4- butanoic acid, XVI) 4 <ωoro-α-[2-[3-[[(2-emoxy-2-oxoedιyl)meώylamino]carbonyl]phenyl]ethyl]-γ-oxo-[ 1,1 - bιphenyl]-4-butanoic acid, XVTJ) 4 ' -chloro-«-[2-[4-(4-moφholinylcarbonyl)phenyl]edιyl]-γ-oxo-[ 1,1 ' -biphenyl]-4-butanoic acid, XVuT) 4'-cWcro-<^[2-(4^[(3-memylbutyl)arn o butanoic acid, %4 SK β s XDO 4 -chloro-α-[2-[4-[[(2-ethoxy-2-oxoethy |) amιno]carbonyl]phenyl]ethyl]-γ-oxo-[ l . l - biphenyl]-4-butanoιc acid. XX) 4 ' <hloro-γ-oxo-α-[2-[4-[[(2-phenylethyl)amino]carbonyl]phenyl]ethyl]-[l,r-bipheny|]-4- butanoic acid. XXI) 4 ' -chloro-γ-oxo-α-[2-[4-(l-pιperidinylcarbonyl ) phenyl]ethylj-[l.r-Biphenyl]-4-butanoιc acid iphenyl]-4-butanoic acid, XXII) 4 ' -chloro-γ-oxo-α-[2-[2-[[(2-phenylethyl)amino]carbonyl]phenyl]edιyl]-(l,r-biphenyl-4- butanoic acid, XXπi) α-(2-(2-[[(2-carboxyed yl)am o]carbonyl]phenyl]edιyl]-4'-chloro-γ-oxo-[l,r-biphenyl]- 4-butanoιc acid, XIV) α-[2-[4[[(cartx)xyme yl)am o]carbonyl]phenyl]edιyl]-4'-chloro-γ-oxo-[l,r-biphenyl]-4- butanoic acid, XXV) 4'-chloro-α-[2[2-[[[2-(4-moφholinyl)ethyl]amino]carbonyl]phenyl]ethyl]-γ-oxo-(l, - biphenyl]-4-butanoic acid, XVI) α-[2-[3-[[(2-carboxyemyl)amino]carbonyl)phenyl]ethyl]-4'-chloro-γ-oxo-[l,l '-biphenyl] -4- butanoic acid, XXVU) 4 '-chloro-α-[2-[3-[[[2 -moφholinyl)eu yl]amino]carbonyl]phenyl]edιyl]-γ-oxo-[ 1,1 '- biphenyl]-4-butanoic acid, XXVTJJ) 4'-chloro-α-(2-4-[[[2-<4-moφholimyl)ethyl]aπιmo]carbonyl]phenyl]ethyl]-γ-oxo-[l ,1 '- biphenyl]-4-butanoic acid, XXTX) 4'<hloro^-{2-[3-[[[2-<dimediylammo)-2-oxoemyl]memylanimo]carrxiny γ-oxo-[ 1 , 1 'biphenyl]-4-butanoic acid. ■ Ub BSTITUTE SHLTT RULE 26 XXX) γ-oxo-*'-(phenylmethoxy)-α-{2-3-(l -piperidinylcarbonyl)phenyl]ethyl]-{l .r-biphenyl]~ - butanoic acid, XXXI) y-oxo-4 ' -(pentyloxy)-α-[2-[3-(l -piperidinylcarbonyl)phenyI]ethyl]-[ l . r-biphenyl]-4- butanoic acid, and XXXJD γ-oxo-α-(2-[2-[(2-oxo- l -piperidinyl)methyl]phenyl]ethyl]-4'-(phenyimethoxy)-[l.r- biphenyI]-4-butanoic acid. General Preparative Methods: The compounds of die invention may be prepared by use of known chemical reactions and procedures. Nevertheless, the following general preparative mediods are presented to aid d e reader in synthesizing die inhibitors, widi more detailed particular examples being presented below in die experimental section describing die working examples. Suitable pharmaceutically acceptable salts of the compounds of die present invention include addition salts formed widi organic or inorganic bases. The salt forming ion derived from such bases can be metal ions, e.g., aluminum, alkali metal ions, such as sodium of potassium, alkaline earth metal ions such as calcium or magnesium, or an amine salt ion, of which a number are known for this purpose. Examples include ammonium salts, arylalkylamines such as dibenzylamine and ^Λ hbenzyie ylenediamine, lower alkylamines such as memyiamine, r-buτyiamine, procaine, lower alkylpiperidines such as N-ethylpiperidine, cycloalkylamines such as cyclohexyiamine or dicyclohexylamine, 1 -adamantylamine, benzadiine, or salts derived from amino acids like arginine, lysine or die like. The physiologically acceptable salts such as die sodium or potassium salts and die amino acid salts can be used medicinally as described below and are preferred. SUBSΠTU SHEET RUL These and other salts which are not necessarily physiologically acceptable are useful in isolating or purifying a product acceptable for the purposes described below. For example, the use of commercially available enantiomerically pure amines such as (+)-cinchonιne in suitable solvents can yield salt crystals of a single enatiomer of the invention compounds, leaving die opposite enantiomer in solution in a process often referred to as "classical resolution." As one enantiomer of a given invention compound is usually substantially greater in physiological effect an its antipode. this active isomer can thus be found purified in either die crystals or the liquid phase. The salts are produced by reacting the acid form of the invention compound widi an equivalent of the base supplying die desired basic ion in a medium in which die salt precipitates or in aqueous medium and tiien lyophilizing. The free acid form can be obtained from die salt by conventional neutralization techniques, e.g., with potassium bisulfate, hydrochloric acid, etc. The compounds of die present invention have been found to inhibit die matrix metalloproteases MMP-3, MMP-9 and MMP-2, and to a lesser extent MMP-1 , and are tiierefore useful for treating or preventing die conditions referred to in he background section. As otiier MMPs not listed above share a high degree of homology widi those listed above, especially in die catalytic site, it is deemed tiiat compounds of the invention should also inhibit such otiier MMPs to varying degrees. Varying the substituents on die biaryl portions of die molecules, as well as diose of die propanoic or butanoic acid chains of d e claimed compounds, has been demonstrated to affect die relative inhibition of die listed MMPs. Thus compounds of this general class can be "tuned" by selecting speci ic substituents such tiiat inhibition of specific MMP(s) associated wid specific padioiogical conditions can be enhanced while leaving non-involved MMPs less affected. 1& The method of treating matrix metalloprotease-mediated conditions may be practiced in mammals, including humans, which exhibit such conditions. The inhibitors of the present invention are contemplated for use tn veteπnary and human applications. For such puφoses, diey will be employed m pharmaceutical compositions containing active ιngredιent(s) plus one or more pharmaceutically acceptable earners, diluents, fillers, binders, and other exciptents. depending on die administration mode and dosage form contemplated. Administrauon of the inhibitors may be by any suitable mode known to those skilled in die art. Examples of suitable parenteral administration include intravenous, intraarticular, subcutaneous and intramuscular routes. Intravenous administration can be used to obtain acute regulaαon of peak plasma concentrations of the drug. Improved half-life and targeting of the drug to die joint cavities may be aided by entrapment of the drug in liposomes. It may be possible to improve die selectivity of liposomal targeting to the joint cavities by incorporation of ligands into die outside of die liposomes tiiat bind to synovial-spectfic macromolecules. Alternatively intramuscular, intraarticular or subcutaneous depot injection widi or without encapsulation of die drug into degradable microspheres e.g., comprising poly(DL-lactide-co-glycolide) may be used to obtain prolonged sustained drug release. For improved convenience of die dosage form it may be possible to use an i p. implanted reservoir and septum such as die Percuseal system available from Pharmacia. Improved convenience and patient compliance may also be achieved by die use of eitiier injector pens (e.g. the Novo Pin or Q-pen) or needle-free jet injectors (e.g. from Bioject Mediject or Becton Dickinson). Prolonged zero-order or other precisely controlled release such as pulsatile release can also be achieved as needed using implantable pumps with delivery of the drug dirough a carmula 2.1 into ie synovial spaces. Examples include the subcutaneously implanted osmotic pumps available from ALZA, such as d e ALZET osmotic pump. Nasal delivery may be achieved by incoφoration of the drug into bioadhesive paniculate carriers ( <200 -*m) such as those comprising cellulose, polyacrylate or polycarbophil. in conjunction with s uitable absoφtion enhancers such as phospholipids or acylcamitines. Available systems include those developed by DanBiosys and Scios Nova. A noteworthy attribute of die compounds of the present invention in contrast to diose of various peptidic compounds referenced in the background section of this application is d e demonstrated oral activity of the present compounds. Certain compounds have shown oral bioavailability in various animal models of up to 90 - 98 %. Oral delivery may be achieved by incoφoration of the drug into tablets, coated tablets, dragees, hard and soft gelatine capsules, solutions, emulsions or suspensions. Oral delivery may also be achieved by incorporation of die drug into enteric coated capsules designed to release die drug into die colon where digestive protease activity is low. Examples include the OROS-CT/Osmet™ and PULSINCAP™ systems from ALZA and Scherer Drug Delivery Systems respectively. Otiier systems use azo-crosslinked polymers tiiat are degraded by colon specific bacterial azoreductases, or pH sensitive polyacrylate polymers that are activated by die rise in pH at d e colon. The above systems may be used in conjunction widi a wide range of available absoφtion enhancers. Rectal delivery may be achieved by incorporation of the drug into suppositories. The compounds of diis invention can be manufactured into die above listed formulations by the addition of various dierapeuticaliy inert inorganic or organic carriers well known to those skilled in die art. Examples of tiiese include, but are not limited to, lactose, com starch or t p derivatives thereof, talc, vegetable oils, waxes, fats, polyols such as polyethylene glycol. water. saccharose, alcohols, glycerin and the like. Various preservatives, emulsifiers, dispersants. tlavorants, wetting agents, antioxidants, sweeteners, colorants, stabilizers, salts, buffers and the iike are also added, as required to assist in die stabilization of the formulation or to assist in increasing bioavailability of the active ingredient(s) or to yield a formulation of acceptable flavor or odor in the case of oral dosing. The amount of die pharmaceutical composition to be employed will depend on the recipient and the condition being treated. The requisite amount may be determined widiout undue experimentation by protocols known to diose skilled in die art. Alternatively, die requisite amount may be calculated, based on a determination of the amount of target enzyme which must be inhibited in order to treat die condition. The matrix metalloprotease inhibitors of die invention are useful not only for treatment of the physiological conditions discussed above, but are also useful in such activities as purification of metalloproteases and testing for matrix metalloprotease activity. Such activity testing can be botii in vitro us g natural or synthetic enzyme preparations or in vivo using, for example, animal models in which abnormal destructive enzyme levels are found spontaneously (use of genetically mutated or transgenic animals) or are induced by administration of exogenous agents or by surgery which disrupts joint stability. EXAMPLES T e following examples are offered for illustrative purposes only and are not intended, nor should d ey be construed, to limit d e invention in any way. 3/ General Procedures: All reactions were performed in flame-dried or oven-dried glassware under a positive pressure of argon and were stirred magnetically unless otherwise indicated. Sensitive liquids and solunons were transferred via syringe or ca nuia and were introduced into reaction vessels tiirough rubber septa. Reaction product solutions were concentrated using a Buchi evaporator unless otherwise indicated. Materials: Commercial grade reagents and solvents were used widiout further purification except that diethyl etiier and tetrahydrofuran were usually distilled under argon from benzophenone ketyl, and mediylene chloride was distilled under argon from calcium hydride. Many of die specialty organic or organometallic starting materials and reagents were obtained from Aidrich, 1001 West Saint Paul Avenue, Milwaukee, WT 53233. Solvents are often obtained from EM Science as distributed by VWR Scientific. Chromatography: Analytical thin-layer chromatography (TLC) was performed on Anaitech' pre-coated glass-backed silica gel GHLF 250 mm plates. Visualization of spots was effected by one of die following techniques: (a) ultraviolet illumination, (b) exposure to iodine vapor, (c) immersion of die plate in a 10% solution of phosphomolybdic acid in edianol followed by heating, and (d) immersion of die plate in a 3% solution of p-anisaldehyde in edianol containing 0.5% concentrated sulfuric acid followed by heating. Column chromatography was performed using 230-400 mesh EM Science* silica gel. **- Instrumentation: Melting points (mp) were determined widi a Thomas-Hoover melting point apparatus and ore uncorrected. Proton ( ! H) nuclear magnetic resonance (NMR) spectra were measured with a General Electric GN-OMEGA 300 (300 MHz) spectrometer, and carbon diirteen ( U C) NMR spectra were measured with a General Electric GN-OMEGA 300 (75 MHz) spectrometer. Most of the compounds systhesized in d e experiments below were analyzed by NMR. and the spectra were consistent widi die proposed structures in each case. Mass spectral (MS) data were obtained on a Kratos Concept l-H spectrometer by liquid-cesium secondary ion (LCIMS), an updated version of fast atom bombardment (FAB). Most of d e compounds sysdiesized in the experiments below were analyzed by mass spectroscopy, and the spectra were consistent widi the proposed structures in each case. General Comments: For multi-step procedures, sequential steps are noted by numbers. Variations within steps are noted by letters. Dashed lines in tabular data indicates point of attachment. Example 1 • Preparation of Compound I Step 1 A solution of o-iodophenylacetic acid ( 19.87 g, 75.83 mmol) in dry tetrahydrofuran (110 mL) was added dropwise over 41 min to a solution of borane in tetrahydrofuran (151 mL of 1 M solution, ca. 151.0 mmoi) which was cooled widi an ice-water bath. The reaction was stirred at 0 • s sπ t wm to 10 3 C for 2 h 15 mm. After the reaction mixture was cooled to 0 °C, it was quenched by cau t ious addition (frodiing!) of 10 (vol.) % acetic acid in metiianol over 20 min. Stirring was continued for 25 min before d e reaction was concentrated on a rotary evaporator. The residue was dissolved in ethyl acetate and washed with saturated ammonium chloride followed by saturated sodium bicarbonate. The organics were dried NajSOj and concentrated to a yellow oil (18.07 g) which was used in the next step without purification. Neat 2-(2-iodophenyl)edιanol ( 17.75 g, 71.55 mmol) was treated dropwise widi phosphorous tribromide (3.5 mL, 36.85 mmol) over 6 min while the reaction vessel was placed in a water bath to modulate die exotiiermic reaction. Stirring was continued for 15 min at room temperature and then for 2 h while the mixture was heated in an oil batii at 100 'C. The reaction was cooled to room temperature, diluted with ether and quenched carefully widi water (frotiiing/exotherm!). The layers were separated, d e organics were washed with saturated sodium bicarbonate and dried (Na 2 SO « ). Concentration gave a yellow oil which was purified by Kugelrohr distillation (140 °C/700 millitorr) to give a colorless oil (19.50 g, 62.71 mmol; 83% yield for above two steps). MS (El) 310, 312 [M] * . Step 2 A dry, 250 mL, round-bottomed flask was equipped widi a stir bar and an argon inlet. The flask was charged widi a suspension of sodium hydride (1.65 g of 95% NaH; -65.1 mmol) in dry THF (25 mL). Diethyi malonate (9.99 g, 62.37 mmol) was added dropwise via syringe over 25 min. Fresh THF (10 mL) was used to wash die addition syringe into die reaction vessel. Stirring was continued for 10 min before rapidly adding die bromide from Step 1 (19.36 g, 62.26 mmol) in THF SDBS (20 mL). The addition syringe was washed ( 10 mL THF) into the reaction. The pale yellow solution was heated at reflux under argon wid overnight stirring. The white suspension was part oned between 10% HC1 and ether. The ediereal layer was washed twice with NaHCO,, dπed (\a : SO.) and concentrated to an oil which was purified by bulb-to-bulb distillation: A forerun fraction (collected at 145 3 C. 700-900 millitorr) was discarded and the bulk distilled at 220 ; C (500-600 millitorr). The bulk fraction was further purified by distilling off lower boiling mateπal at 150 °C (300-500 millitorr) to leave d e clean desired product in the pot ( 15.28 g, 39.16 mmol; 63%yield). TLC (developed twice in hexanes-ediyl acetate. 20:1): Pγ= 0.33. Step 3 A 2-L, diree-necked, round-bottomed flask was equipped widi a mechanical stirrer, a tiiermometer and an argon inlet The flask was charged widi a solution of 4-chlorobiphenyl (48.30 g, 0.256 mol) in dichloromethane (500 mL, freshly opened bottle). Bromoacetyl bromide (23 mL, -53.3 g, -0.26 mol) was added via syringe and die solution was cooled widi an ice water bath to an internal temperature of 3 'C. The diermometer was temporarily removed and AiCl 3 was added portionwise over 5 min. The internal temperature rose to 10 "C and white gas evolved from die opaque olive green reaction mixture. After 24 h of stirring, die reaction was quenched by cautiously pouring into cold 10% HC1 (1 L). The organic layer became cloudy yellow green. Chloroform was added to help dissolve solids, but die organic layer never became transparent. The organics were concentrated on a rotary evaporator and were dried further under high vacuum. The crude product was a pale green solid (-82 g) which recrystallized from hot ediyl acetate to give 1 -(2-bromoedianone)-4-(4-chiorophenyl benzene as brown needles (58.16 g). Concentration of die ■ ht mother liquor followed by addition of hexanes delivered a second crop of crystals ( 1 1 06 g hich gave an NMR spectrum identical to mat of the first crop. The total yield of the title product was 87%. TLC (hexanes-dichloromethane. 2: 1 ): R, = 0.30. Step 4 A dry, 250 mL, round-bottomed flask was equipped widi a stir bar and an argon inlet. The flask was charged with a suspension of sodium hydride (1.07 g of 95% NaH; -42.4 mmol) in dry THF ( 100 mL) and was cooled with an ice-water badi. A solution of the malonate from Step 2 ( 15.25 g, 39.08 mmol) in THF (30 mL) was added dropwise over 30 min. Fresh THF (10 mL) was added by syringe to the reaction vessel and die cooling batii was removed. After die reaction stirred for 10 min, d e bromomethyl ketone from Step 3 was added in a single portion. The orange mixture was stirred under argon overnight while slowly warming to room temperature. The reaction mixture was cautiously added to 10% HCl. The layers were separated and die aqueous layer was extracted widi ediyl acetate. The combined organics were washed sequentially wid 10% HCl and saturated sodium bicarbonate. The combined organics were dried (Na,SO«) and concentrated to afford an orange-brown oil (24.41 g). This crude material was used in d e next step widiout purification. The crude oil (24.19 g, -39.08 mmol) was dissolved in THF (150 mL) and absolute edianol ( 100 mL). To this mixture was added NaOH solution ( 10 mL of 50 wt. % aqueous NaOH, -0.125 mol) and die reaction was stirred under argon overnight at room temperature. The mixture was brought to pH~l by adding 10% HCl and the cloudy, yellow solution was extracted with ethyl acetate. The combined organics were washed with brine, dried (Na,S04) and concen t rated t o an orange foam ( 22.06 g). This material was used in the next step without purification. TLC (chloroform-medianol, 20: 1 wid trace amount of acetic acid): R,- 0.12. Step 5 The diacid product from step 4 (22.06 g) was dissolved in 1 ,4-dioxane (400 mL) and was held at reflux under argon overnight. Concentration gave d e crude product as a yellow solid (19.50 g) which was recrystallized from chloroform to deliver two crops of die title compound Example 1 as a fluffy solid (11.55 g, 22.26 mmol; 7% overall yield) after overnight drying in a vacuum oven at 66 ' C. TLC (chloroform-medianol, 20: 1 with trace amount of acetic acid): R_- s 0.54. Compound I Step 6 A portion of the acid from Step 5 (405.7 mg, 0.78 mmol) was dissolved in climediylsuLfbxide (3.0 mL). Trieuryl amine (0.34 mL) was added followed by palladium (IT) aceute (20.3 mg, 0.09 mmol), l,3-bis(diphenylphosphino)propane (35.2 mg, 0.085 mmoi) and phenethylamine (1.42 g, 11.7 mmol). Carbon monoxide was bubbled dirough die solution for five minutes. The solution was placed under a carbon monoxide atmosphere and was heated in an oil -41 BSTITUTE SHEET RULE 2€ bath at 70-75 S C overnight. The mixture was cooled to room temperature, dilu t ed wi t h ethyl acetate (50 mL) and washed with 10% HCl followed by water. The aqueous phase was back-extracted wi t h ethyl acetate and the combined organics were dried (MgSO and concentrated to a yellow-orange solid. Purification by flash chromatography (chloroform-medianol, 95:5) gave an off-white solid which was recrystallized from ethyl acetate hexanes to give the tide compound (219.7 mg, 0.41 mmol; 53% yield). Anal , (for C JJ H JO O^I) C: caicd, 73.39; found, 73.1 1. H: calcd. 5.60: found. 5.40. N: calcd, 2.59; found, 2.32. The examples in Table I were prepared by d e palladium-mediated carbonylation metiiod of Example I widi die appropriate amine in place of phenediyl amine. Furthermore, die requisite isomeric iodide precursors were prepared by the metiiod of Example 1 using m- or p-iodophenylacetic acid as starting material. TABLE I SBBSi HE SHEET 0HLE2 0 SBBSΉΠΠE #» Example 22 - Preparation of Compound XXII Compound XXII The acid from Example 7 (200 mg, 0.37 mmol) was dissolved in edianol (15 mL) and was treated widi IN NaOH (1.8 mL, 1.8 mmol). The mixture was stirred at room temperature over the weekend. The reaction was concentrated to dryness and die residue was partitioned between chloroform and 10% HCl. The layers were separated and d e aqueous phase was extracted again with chloroform. The combined organics were dried (Na : SO,) and concentrated to an off-white solid. The crude product was recrystallized from ethanol-hexane to give the product as off-white crystals (65 mg, 0.128 mmol; 35% yield). MP 189.5-190.5 °C. Example 23 • Preparation of Compound XXJJJ The acid from Example 8 (180 mg, 0.34 mmol) was saponified according to die general metiiod of Example 22 to give the diacid product as a yellow solid (142 mg, 85% yield). MP 71-75 °C. Ή Example 24 - Preparation of Compound XTV Compound XIV The acid from Example 19 (104 mg, 0.199 mmol) was saponified according to d e general method of Example 22 to give die diacid product as colorless crystals (79 mg, 80% yield). MP 164.5- 165.5 =C. Example 25 - Preparation of Compound XXV Step 1 A sample of die iodo acid from Example I, Step 5 (1.0 g, 1.93 mmol) was suspended in dry dichloromediane (20 mL). To d e stirred suspension was added benzyl alcohol (0.44 mL, 4.05 mmol), DCC (0.6 g, 2.89 mmol) and DMAP (50 mg, 0.39 mmol). The yellow suspension was stirred overnight at room temperature. The mixture was diluted wid hexanes (60 mL) and water (5 mL). The mixture was stirred and filtered and d e filter cake was washed wid hexanes. The organics were separated, dried (MgSO«) and concentrated to an oil. Flash chromatography (gradient 4 SUBSTITUTE SHEE elution. hexane-ethyl acetate, 9: 1 to 1 : 1) gave the purified ester as an oil (0.95 g, 1.56 mmol: 81 ' yield). TLC (hexanes-ethyl acetate, 1 : 1 ): R = 0.64. Step 2 The iodo ester from Step 1 (0.910 g, 1.49 mmol) was subjected to the palladium-mediated carbonylation metiiod of Example 1 widi water in place of phenediyl amine to give the half acid ester product (475 mg, 0.90 mmol; 61% yield). MS (FAB-LSDMS) 527TM+H] * Step 3 The half acid ester from Step 2 (0.46 g, 0.87 mmol) was dissolved in dichlorometiiane (8 mL). To the solution was added 4-{2-aminoethyl)moφholine (0.13 g, 0.96 mmol) and l-hydroxybenzotriazole (0.12 g, 0.87 mmol). The flask was washed down widi dichloromethane (2 mL) and cooled in an ice bath. 4-Methylmorpholine was added (97 mg, 0.96 mmol) followed by 1 -(3-dimeu^aπι opropyl)-3-ed yl-carbodiimide hydrochloride (0.18 g, 0.96 mmol). The yellow solution was warmed to room temperature with overnight stirring. The mixture was diluted widi dichlorometiiane (40 mL) and washed wid water. The aqueous layers were back-extracted widi dichloromethane. The combined organics were dried (Na,SO 4 ) and concentrated. Purification by ft? SUBSTITUTE SHEET MILE 26) flash chromatography (chloroform-methanol, 95:5) gave the product as a pale ellow oil (0 . 438 g. 0.685 mmol; 78% yield). TLC (chloroform-methanol. 9: 1 ): - 0.70. Compound XXV Step 4. The acid from Step 3 (0.15 g , 0.47 mmol) was saponified according to die general method of Example 22 to give die product as cream colored foam (65.2 mg, 52%). MP 127-129 °C. Example 26 • Preparation of Compound XXVI Step 1 The meta-iodo isomer of Example 1 , Step 5 (i.e., prepared according to die procedure of Example 1 beginning widi -iodophenyl acetic acid; 2.0 g, 3.86 mmol) was dissolved in 1,2- dichloroediane (8 mL). To die solution was added edianol (0.68 mL, 11.57 mmol) and several drops of concenrrated sulfuric acid. The solution was held at reflux overnight The mixture was cooled, loaded onto a silica column and flash chromatographed (hexanes-ediyl acetate, 3:1) to give he product as a yellow oil (2.142 g) which retained solvent by proton NMR. TLC (chloroform-methanol, 10:1): R^ 0.91. Ψf Step 2 The iodo ester from Step 1 (2.1 g, 3.84 mmol) was subjected to the palladium-mediated carbonylation method of Example I with water in place of phenediyl amine to give d e half acid ester product ( 1.1 g, 2.37 mmol; 62% yield). TLC (1 : 1 hexane-ethyl acetate with 1 % acetic acid): R^= 0.70. Compound XXVI Step 3 The half acid ester from Step 2 was converted to Example 26 using b-alanine ediyl ester in d e general metiiod of Example 25. MP 261 -261 °C. The examples in Table II were prepared by die general multi-step metiiod of Example 25 starting widi die appropriate iodo acid and amine precursors. # TABLE II Example 30 - Preparation of Compound XXX Step 1 A one-necked, 1000-mL, round-bottomed flask equipped widi an argon inlet adapter was charged with 500 mL CH,C1„ 4-phenylphenol acetate (50.0 g, 235 mmol), bromoaceryl bromide (73.2 g, 31.6 mL, 363 mmol) and cooled to 0 β C while aluminum trichloride (94.2 g, 707 mmol) was added in small portions ca. over 5 min. The resulting mixture was stirred for 30 min at 0 °C and 12 h at room temperature. The reaction mixture was added to a cold 10% HCl solution (500 mL), and extracted tiiree times with 200-mL portions of ediyl acetate. The organic phase was dried over MgSO , filtered, and concentrated to provide a black solid. Recrystallization from ethyl > acetate-hexanes afforded 44.3 g ( 56%) of the desired compound as a brown solid. TLC (hexanes-ethyi acetate, 9: 1 ) R = 0.14. Step 2 The desired compound was synthesized from the product of Step 1 above by the general procedure in Example I . TLC (hexanes - ediyl acetate, 3 : 1 ) R / = 0.49. Step 3 A tetrahydrofuran (400 mL) and ethanol (50 mL) solution of die product from Step 2 ( 18.4 g) was treated with . 2 C0 3 and stirred under argon at room temperature overnight. Because a significant amount of starting material remained, die volume of die reaction was reduced by one half and additional K 2 CO, (12 g) was added. The reaction was complete after 3 h. The reaction was concentrated and acidified with 10% HCl. The product was extracted with ediyl acetate, dried (Na,SOJ and concentrated to a brown oily residue. Purification by flash chromatography (hexanes - ediyl acetate, 3: 1) gave die product as a yellow oil (14.8 g; 86%). TLC (hexanes - ethyl acetate, 3:1) R, - 0.20. Ψi ππ cooled with an ice-water badi and treated widi a solution of the phenol from Step 3 (3.4 g, 5.66 mmol) in dry DMF (20 mL). The mixture was warmed to room temperature and benzyl bromide (3.4 mL, -28.3 mmol) was added in a single portion. The flask was stirred at room temperature overnight. The mixture was diluted with 10% HCl and extracted widi ediyl acetate. The organics were dried (MgSO and concentrated to a yellow oil. Flash chromatography (gradient elution. hexanes-etiiyl acetate, 9: 1 to 3:2) gave die intermediate diester (3.32 g). This material was dissolved in a mixture of THF (25 mL), edianol (100 mL) and IN NaOH (22 mL) and the solution was stirred overnight The reaction mixture was concentrated and d e residue was partitioned between 10% HCt and ethyl acetate. The organics were dried (Na-SO 4 ) and concentrated to a white solid (2.51 g). This diacid was dissolved in 1,4-dioxane (250 mL) and die solution was held at reflux overnight. The solution was cooled and concentrated to a yellow-white solid (2.36 g). Recrystallization from ethyl acetate gave pale yellow crystals ( 1.77 g). MP 173- 174 °C TLC (hexanes - ediyl acetate, 3 : 1 with a trace of acetic acid) R/- a 0.43. 19 Step 5. Example 30 was prepared by the palladium-mediated carbonylation mediod of Example 1 widi piperidine as the nucleophile. MP 100-102.5 °C. Example 31 - Preparation of Compound XXXI Example 31 was prepared by die general procedure of Example 30 using iodopeniane in die alkylation step and piperidine as the nucleophile in the palladium-mediated carbonylation. MP 105.5-107.5 a C. Compound XXXTJ 1°, Example 32 • Preparation of Compound XXXII Example 32 was prepared by die general procedure of Example 30 using die appropriate isomeric iodo precursor. MP 168-169 °C. Example 33 Biological Assays of Invention Compounds P218 Quenched Fluorescence Aaaav for MMP Inhibition: The P218 quenched fluorescence assay (Microfluorometric Profiling Assay) is a modification of that originally described by Knight et al., FEBS Lett. 29£, 263, 1992 for a related substance and a variety of matrix metalloproteinases (MMPs) in cuvettes. The assay was run widi each invention compound and the tiiree MMPs, Mmp-3, MMP-9 and MMP-2, analyzed in parallel, adapted as follows for a 96-well microtiter plate and a Hamilton AT* workstation. P218 Fluorogenic Substrate: P218 is a synthetic substrate containing a 4-acetyl-7- methoxycoumarin (MCA) group in die N-terminal position and a 3-[2, 4-dinitrophenyi]-L-2,3- diaminopropionyi (DPA) group internally. This is a modification of a peptide reported by Knight ( 1992) dal was used as a substrate for matrix metalloproteinases. Once the P218 peptide is cleaved (putative clip site at die Ala-Leu bond), die fluorescence of die MCA group can be detected on a fluorometer widi excitation at 328 nm and emission at 393 nm. P218 is currently being produced BACHEM exclusively for Bayer. P218 has the structure: H.MCA.Pro-Lys-Pro-Leu-^to-Z.««-DPA-Ala-Arg-NH2 (MW 1332.2) Recombinant Human CHO Stromervsin fMMP-3ϊ Recombinant Human CHO Pro-MMP-3. Human CHO pro-stromelysin-257 (pro-MMP-3) was expressed and purified as described by Housley, et al., J. Biol. Chem. 2$ 4481, 1993. 5t> Activation ofPro-MMP-3. Pro-MMP-3 at 1.72 μM (100 μg mL) in 5 M Tris at pH 7.5. 5 mM CaClj, 25 mM NaCl, and 0.005% Brij-35 (MMP-3 activation buffer) was activated by incubation with TPCK (N-tosyl-(L)-phenylalanine chloromethyl ketone) trypsin ( 1 : 100 w/w to pro- MMP-3) at 25 °C for 30 min. The reaction was stopped by addition of soybean trypsin inhibitor (SBTT: 5: 1 w/w to trypsin concentration). This acuvation protocol results in d e formation of 45 kDa active MMP-3, which still contains die C-terminal portion of die enzyme. Preparation of Human Recombinant Pro-Gelatinase A (MMP-2>: Recombinanx Human Pro-MMP-2: Human pro-gelatinase A (pro-MMP-2) was prepared using a vaccinia expression system according to die method of Fridman, et al., J Biol. Chem. 26 , 15398, 1992. Activation of Pro-MMP-2: Pro-MMP-2 at 252 mg/mL was diluted 1 :5 to a final concentration of 50 μg mL solution in 25 mM Tris at pH 7.5, 5 mM CaCl,, 150 mM NaCl, and 0.005% Brij-35 (MMP-2 activation buffer). acetate (APMA) was prepared in 10 mM (3.5 mg/mL) in 0.05 NaOH. The APMA solution was added at 1/20 die reaction volume for a final AMPA concentration of 0.5 mM, and the enzyme was incubated at 37 °C for 30 mm. Activated MMP-2 (15 mL) was dialyzed twice vs. 2 L of MMP-2 activation buffer (dialysis membranes were pre-treated with a solution consisting of 0.1% BSA in MMP-2 activation buffer for 1 min.. followed by extensive H 2 O washing). The enzyme was concentrated on Centricσn concentraton (concentrators were also pre-treated a solution consisting of 0.1% BSA in MMP -2 activation buffer for 1 min.. followed by washing with H 2 O, ώen MMP-2 activation buffer) with re-dilution followed by re-concentration repeated twice. The enzyme was diluted to 7.5 mL (0.5 times the original volume) wid MMP-2 activation buffer. 57 Preparation of Human Recombinant Pro-Gelarinaae B fVTMP-9^: Recombinant Human Pro-MMP-9: Human pro-gelatinase B (pro-MMP-9) derived from L ' 937 cDNA as described by Wilhelm, et al. J Biol. Chem. 2^4, 17213, 1989 was expressed as the full-length form using a baculovirus protein expression system. The pro-enzyme was purified using metiiods previously described by Hibbs, et al. J. Biol. Chem. 26J2, 2493, 1 84. Activation of Pro-MMP-9: Pro-MMP-2 20 μg/mL in 50 mM Tris at pH 7.4, lOmM CaCU, 150 mM NaCl, and 0.005% Brij-35 (MMP-9 activation buffer) was activated by incubation widi 0.5 mM -aminophenylmercuric acetate (APMA) for 3.5 h at 37 °C. The enzyme was diaiyzed against the same buffer to remove die APMA. Instrumentation: Hamiltion Microlab AT Plus: The MMP-Profiling Assay is performed robotically on a Hamilton MicroLab AT Plus*. The Hamilton is programmed to: (1) serially dilute up to 1 1 potential inhibitors automatically from a 2.5 mM stock in 100% DMSO; (2) distribute substrate followed by inhibitor into a 96 well Cytofluor plate; and (3) add a single enzyme to die plate with mixing to start d e reaction. Subsequent plates for each additional enzyme are prepared automatically by beginning the program at the substrate addition point, remixing d e diluted inhibitors and beginning die reaction by addition of enzyme. In this way, all MMP assays were done using die same inhibitor dilutions. Millipore Cytofluor IL Following incubation, die plate was read on a Cytofluor LT. fluorornetric plate reader widi excitation at 340 nM and emission at 395 tiM with die gain set at 80. B iHeπ: 5 Microfluorometric Reaction Buffer ( MRB): Dilution of test compounds, enzymes, and P21 substrate for die microfluorometric assay were made in microfluorometric reaction buffer consisting of 50 mM 2-(N-mo holino)ethanesulfonic acid (MES) at pH 6.5 widi 10 mM CaCL, 150 mM NaCl. 0.005% Brij- 5 and 1% DMSO. eJtadj.: MMP Microfluorometric Profiling Assay. The assay is done with a final substrate concentration of 6 μM P218 and approximately .5 to .8 nM MMP widi variable drug concentrations. The Hamilton is programmed to serially dilute up to 1 1 compounds from a 2.5 mM stock (100% DMSO) to lOx me final compounds concentrations in d e assay. Initially, die instrument delivers various amounts of microfluoromentric reaction buffer (MRB) to a 96 tube rack of I ml Marsh dilution rubes. The instrument men picks up 20 μl of inhibitor (2.5 mM) from the sample rack and mixes it widi a buffer in row A of the Marsh rack, resulting in a 50 μM drug concentration. The inhibitors are then serially diluted to 10, 5, 1, .2, .05 and .01 μM. Position 1 on die sample rack contains only DMSO for the "enzyme-only" wells in the assay, which results in no inhibitor in column 1, rows A dirough H. The instrument men distributes 107 μl of P218 substrate (8.2 μM in MRB) to a single 96 well cytofluor microuter plate. The instrument re-mixes and loads 14.5 μl of diluted compound from rows A to G in the Marsh rack to corresponding rows in die microuter plate. (Row H represents die "background" row and 39.5 μl of MRB is delivered in placed of drug or enzyme). The reaction is started by adding 25 μl of the appropriate enzyme (at 5.86 times die final enzyme concentration) from a BSA treated reagent reservoir to each well, excluding Row H, die "background" row. (The enzyme reservoir is pretreated with 1% BSA in 50 mM Tris, pH 7.5 containing 150 mM NaCl for 1 hour at room temp., followed by extensive H : 0 washing and drying at room temp.). After addition and mixing of the enzyme, the plate is covered and incubated for 25 min. at 37°C. Additional enzymes are tested in die same manner by beginning the Hamilton program with the distribution of P218 substrate to die microtiter plate, followed by re-mixing and distribution of the drug from die same Marsh rack to die microtiter plate. The second (or third, etc.) MMP to be tested is then distributed from a reagent rack to the microtiter plate widi mixing, prior to covering and incubation. This is repeated for all additional MMP's to be tested. IC50 and Ki Determination in Microfluorometric Assay: Data generated on the Cytofluor TJ is copied from an exported ".CSV" file to a master Excel spreadsheet Data from several different MMPs (one 96 well plate per MMP) were calculated simultaneously. The percent inhibition is determination for each drug concentration by comparing die amount of hydrolysis (fluorescence units generated over 25 minutes of hydrolysis) of wells containing compound widi die "enzyme only" wells in column 1. Following subtraction of die background the percent inhibition was calculated as: ((Control values • Treated valuesyControl values) x 100 Percent inhibitions were determined for inhibitor concentrations of 5, 1, 0.5, 0.1, 0.02,0 .005 and, 0.001 μM of drug. Linear regression analysis of percent inhibition versus log inhibitor concentration was used to obtain IC W values. K,'s were calculated automatically for each enzyme tested based upon the equation: κ-((K m xiO o yfA. + rsi) *f SKBSΠΠ where [S] » substrate concentration - 6 μM. This is die mediod of Williams, et al., Methods Enzym. 62, 437, 1979. TABLE m ss Other embodiments of die invention will be apparent to those skilled in die art from a consideration of this specification or practice of die invention disclosed herein. It is intended that die specification and examples be considered as exemplary only, widi die true scope and spirit of die invention being indicated by d e following claims. 5»

Claims: We claim A matrix metailoproteinase inhibiting compound having the general formula: wherein T is benzyloxy, pe„ty| 0X y, or CI , Md R a is χiκai fom ^ ^ ^.^ of 67 2. A composition having matrix metalloprotease inhibitory activity, comprising a compound of claim I and a pharmaceutically acceptable carrier. 3. A method of inhibiting matrix metalloprotease activity in a mammal comprising administration of an effective amount matrix metalloprotease inhibitor compound of claim I to said mammal. 4. The mediod of claim 3 wherein said mammal is a human. 5. A method of treating a mammal comprising administering to die mammal a matrix metalloprotease inhibiting amount of a compound according to claim 1 sufficient to: (a) alleviate die effects of osteoarthritis, rheumatoid arthritis, septic arthritis, periodontal disease, comeal uiceration, proteinuria, aneurysmal aortic disease, dystrophobic epidermolysis, buliosa, conditions leading to inflammatory responses, osteopenias mediated by MMP activity, tempero mandibular joint disease, demyelating diseases of the nervous system; (b) retard tumor metastasis or degenerative cartilage loss following traumatic joint injury, (c) reduce coronary thrombosis from athrosclerotic plaque rupture; or (d) effect birth control. 6 6. The method of claim 5 wherein the effect is alleviation of osteoarthritis. 7. The method of claim 5 wherein die effect is retardation of tumor metastasis. 8. A compound having die formula: fi

Inventors: WOLANIN DONALD J, WOLANIN, DONALD, J.
Applicants: BAYER AG, WOLANIN DONALD J, BAYER CORPORATION, WOLANIN, DONALD, J.
Application year: 1997
Main classification: C07C235
IPC codes: A61K31, A61P1, A61P9, A61P27, A61P29, A61P43, C07C17, C07C45, C07C59, C07C69, C07C235, C07D295