PROBE FOR THE DETECTION OF CERVICAL NEOPLASIAS USING FLUORESCENCE SPECTROSCOPY

Abstract: A probe, and an apparatus incorporating the probe, for detecting tissue abnormality, particularly precancerous cervical tissue, through fluorescence spectroscopy are disclosed. In vivo fluorescence excitation and detection can be accomplished easily and accurately using a probe whose tip conforms to the surface of the cervix. In addition, a higher number of fiber pairs provides more information and, therefore, more accurate diagnosis.

Description: DESCRIPTION PROBE FOR THE DETECTION OF CERVICAL NEOPLASIAS USING FLUORESCENCE SPECTROSCOPY BACKGROUND OF THE INVENTION The field of the invention relates to optical probes used for the screening and diagnosis of tissue abnormalities, and apparatus incorporating such probes. In particular, the invention relates to fluorescent spectroscopy probes and apparatus to detect cancerous and precancerous tissues of the cervix. Cervical cancer is the second most common malignancy in women worldwide, exceeded only by breast cancer and in the United States, it is the third most common neoplasm of the female genital tract - 15,000 new cases of invasive cervical cancer and 55,000 cases of carcinoma in situ (CIS) were reported in the U.S. in 1994. In 1994, an estimated 4,600 deaths occurred in the United States alone from cervical cancer. However, in recent years, the incidence of pre-invasive squamous carcinoma of the cervix has risen dramatically, especially among young women. Women under the age of 35 years account for up to 24.5% of patients with invasive cervical cancer, and the incidence is continuing to increase for women in this age group. It has been estimated that the mortality of cervical cancer may rise by 20% in the next decade unless further improvements are made in detection techniques. The mortality associated with cervical cancer can be reduced if this disease is detected at the early stages of development or at the pre-cancerous state (cervical intraepithelial neoplasia (CIN) ) . A Pap smear is used to screen for CIN and cervical cancer in the general female population. This technique has a false-negative error rate of 15-40%. An abnormal Pap smear is followed by colposcopic examination, biopsy and histologic confirmation of the clinical diagnosis. Colposcopy requires extensive training and its accuracy for diagnosis is variable and limited even in expert hands. A diagnostic method that could improve the performance of colposcopy in the hands of less experienced practitioners, eliminate the need for multiple biopsies and allow more effective wide scale diagnosis could potentially reduce the mortality associated with cervical cancer. Recently, fluorescence, infrared absorption and Raman spectroscopies have been proposed for cancer and precancer diagnosis. Many groups have successfully demonstrated their use in various organ systems. Auto- and dye-induced fluorescence have shown promise in recognizing atherosclerosis and various types of cancers and precancers. Many groups have demonstrated that autofluorescence may be used for differentiation of normal and abnormal tissues in the human breast and lung, bronchus and gastrointestinal tract. Fluorescence spectroscopic techniques have also been investigated for improved detection of cervical dysplasia. Despite these advances, there remains a need for diagnostic tools with improved accuracy and ease of use. Such methods will permit more early diagnosis, more effective patient management and, potentially, reduce mortality. SUMMARY OF THE INVENTION Thus, it is an objective of the present invention to provide improved tools for the early detection of neoplasia. In particular, it is an objective of the present invention to provide improved spectroscopic probes for the identification of abnormal cervical tissue, thereby providing a rapid, accurate and simple method for detecting neoplastic cervical tissue. It is another objective of the present invention to provide an improved apparatus incorporating the improved probes for the identification of abnormal cervical tissue. In satisfying these and other objectives, there is provided a probe for the optical diagnosis of tissue abnormalities at the cervix, and an apparatus incorporating the probe. In one embodiment, the present invention provides for the detection of tissue abnormality in a tissue sample in vivo by illuminating a cervical surface sample with a series of electromagnetic radiation wavelengths. Also provided are means to measure the fluorescence intensity spectra emitted from the tissue as a result of illumination with the electromagnetic radiation. An optical probe for cervical examination is provided comprising (a) a probe casing having an elongate portion and at least two openings, wherein one of said openings is disposed at a distal tip of said elongate portion of said casing; (b) a plurality of optical fiber pairs, each of said pairs comprising an excitation fiber and a collection fibers, said optical fibers entering said casing at the non-tip opening and traversing said casing to said tip opening; and (c) an optically transparent window with a surface structured to conform to the surface of the human cervix, said window covering said tip opening. In an exemplary embodiment, the probe window is substantially conical. In another embodiment, the number of fiber pairs is 31 or 357. In yet another embodiment, the window is comprised of quartz or BK7 glass. These and other features and advantages of the present invention will become apparent to those of ordinary skill in this art with reference to the appended drawings and following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1A shows the cartesian coordinates for a 31 fiber pair probe tip configuration, in accordance with an embodiment of the present invention. FIG. IB shows the actual distribution for the 31 fiber pair probe tip configuration of FIG. 1. FIG. 2 shows the actual distribution for a 357 fiber pair probe tip configuration, in accordance with another embodiment of the present invention. FIG. 3 shows a cross-section of a 42 mm probe window (NA=0.22 equispaced fibers), in accordance with an embodiment of the present invention. FIG. 4A shows a cross-section of a 40 mm probe window (height 12 mm) , in accordance with another embodiment of the present invention. FIG. 4B shows a cross-section of a 10 mm probe window, in accordance with yet another embodiment of the present invention. FIG. 5 shows a transverse section of a 40 mm probe tip (1) , in accordance with an embodiment of the present invention. Also shown are a fiber pair (2) , epoxy (3) , quart window (4) , glass tubes (500 μm ID, 2 mm OD) holding the fiber pairs 2 mm (5) and aluminum casing (6) . FIG. 6 shows a transverse section of a 10 mm probe tip (1) , in accordance with another embodiment of the present invention. Also shown are heat shrink tubing (2), a fiber pair (3), aluminum casing (4), quartz window (5) and aluminum plate (500 μm holes drilled every 2 mm) (6). FIG. 7 is a diagram of an exemplary fluorescence spectroscopy diagnostic apparatus, incorporating the probe of the present invention. FIG. 8 illustrates fiber arrangement by couplers at the excitation and collection ends of the probe of the present invention: collection cross section (1) ; excitation cross section (2) ; chromex 250 IS (31 emission fibers) (3) ; filter and focusing optics (4) ; quartz tip (5) ; sample (6) ; N2/Dye laser (31 excitation fibers) (7) . DETAILED DESCRIPTION . Introduction Clinical detection of neoplasias can be divided into two different kinds of analysis. First, screening provides a way to identify suspicious samples taken from a rather large pool of subjects. Subjects may be from the population as whole or they may be part of a group identified as having a higher than average risk for one or more neoplasms. It is desirable that, because of the sheer number of tests, screening assays be relatively rapid, easy to conduct and inexpensive. It also is essential that the exhibit a low false-negative rate. Once patients have been screened, it is necessary to proceed with more detailed testing that can be referred to generically as diagnosis. In diagnosis, the neoplastic nature of the sample is confirmed and, in addition, further information on the type and degree of dysplasia is obtained. This provides the clinician with an understanding of the disease state necessary to begin a treatment regimen. For diagnosis, cost, ease of application and rapidity are less important, though always desirable. It is important, however, that diagnostic procedures be accurate with respect to the kind of neoplasm identified and its clinical stage. The present invention permits accurate measurements for the second kind of detection, diagnosis. Present probe technology is limited by both the number of fibers that can be concentrated at the probe tip, both for excitation and detection purposes. In addition, the non- conforming shape of prior art probe tips precludes accurate fluorescent measurements. It is believed that by using a more fibers and a probe tip that conforms to the surface of the cervix, the accuracy of fluorescent measurements can be improved. In addition, the possibility for automation has further benefits in terms of time and expense. A. Probe Design The probe of the present invention is designed primarily for use in the vaginal cavity but can, in theory, be used in other situations including oral, rectal and intraoperative procedures. Regardless, the probe will comprise at least a portion that is elongated and suitable for insertion into any of the aforementioned body cavities or into a surgical field. The elongation is formed from the housing of the probe. The housing preferably is rigid but, in some circumstances, may be flexible. Regardless of the rigidity, the housing must provide sufficient protection for optical fibers disposed therein. The housing also should be durable in that it can withstand harsh chemical treatment, i.e., chemical sterilization and disinfection. Typical materials include various plastics, metals, resins, polymers, and the like. Preferred housing materials are carbonized (black) epoxy and aluminum. Further, the housing may be encased in a protective coating such as heat-shrink tubing. The housing also will comprise at least two openings, the first of which permits entry fiber optic bundles. In addition, the housing comprises a cavity connecting the two openings. Thus, the fiber optic bundles pass though the first opening, traverse the cavity and are present at the second opening. The bundles are comprised of fiber pairs, comprising excitation and detection fibers. The fibers typically are comprised of a central core and a cladding surrounding the core. Any optical fiber can be employed in the disclosed probe and those of skill in the art are able to select fibers having varying properties depending on the particular use envisioned. The disposition of fibers at the second opening is an important aspect of the present invention. In an exemplary embodiment, 31 fiber pairs are arranged as shown in FIG. IB. In another exemplary embodiment, 357 fiber pairs are arranged as shown in FIG. 2. It is desirable that as large a number of fiber pairs as possible be used; the more fiber pairs, the more information provided by the probe. There is a point of diminishing returns, however, when crowding of the fibers causes cross-talk. To a certain extent, cross-talk can be reduced by altering the signal energy and by altering the timing of the detection. Again, those of skill in the art will be able to select these parameters, based on the particular endeavor and the fiber configuration. In order to achieve the proper configuration, it may be desirable to employ a perforated plate, the perforations of which are used to physically fix fiber pairs. The plate would be located at the second opening and secured by any available means to the housing. Alternatively, the fiber pairs may be encased in glass tubes that, when packed together, provide the appropriate spacing and arrangement. Tip configurations are illustrated in FIG. 5 and FIG. 6. The second opening is covered by a substantially optically transparent window. Materials used for the window may include quartz, glass, fused silica, sapphire or other suitable substantially optically transparent material. Preferred materials are quartz and BK7 glass. In one embodiment of the present invention, the window surface is shaped to conform with the sample surface, for example, the cervix. Because the cervix has an involuted structure, its desirable that the window surface be evoluted, i . e . , the inverse or mirror of the cervix. While the precise structure of the window may vary somewhat, it generally will be conical in shape. In this way, the gap between the window surface and the sample across the probe tip will substantially be negated. Depending on the size of the probe tip, only a portion of the window may be conical in shape, the remainder being flat or having a curve that conforms to the area about the cervical opening. The window width and thickness will vary widely depending on the sample target. In general, for cervical probes, the window will range from about 10 mm to about 40 mm in width and the thickness will range from about 4 mm to about 10 mm. Examples are shown in FIG. 3 and FIG. 4A and FIG. 4B. B. Apparatus for Determining Fluorescent Spectra The probe of the present invention is used as part of an apparatus for measuring tissue fluorescence. The apparatus comprises a probe, an electromagnetic radiation generator, a fluorescent spectrum detector and a means for analyzing the spectra in relation to said electromagnetic radiation. Typically, the means for analyzing the spectra will be a computer equipped with the proper interpretive software, which can be selected by those of skill in the art depending on the particular purpose to which it is applied. FIG. 7 is an exemplary spectroscopic system for collecting and analyzing fluorescence spectra from tissue samples, in accordance with the present invention. An important part of the apparatus is means by which the fiber pairs are connected to the electromagnetic radiation generator and the fluorescent spectrum detector. These coupling means arrange the fiber pairs into a particular cross-section. For the coupling means attaching the fiber pairs to the electromagnetic radiation generator, the fiber pairs may be arranged in any fashion but typically, to save space, the fibers are packed as a bundle. The coupling means for the fluorescent spectra detector preferably arrange the fiber pairs in a linear fashion. This is needed because fluorescent spectra detectors often have a linear (vertical) aperture through which light samples are fed. See FIG. 8. APPENDIX I: REFERENCES The disclosures of the following publications, patents and applications are expressly incorporated herein by reference, as are any of the other references mentioned above: A two-stage fluorescence diagnostic method is disclosed in detail in application Serial No. 08/060,432, filed May 12, 1993, and is assigned to the same assignee as the present invention. A patent, U.S. 5,125,404, entitled "Apparatus and Method for Obtaining Spectrally Resolved Spatial Images of Tissue" (Kittrell et al . ) was filed on November 21, 1989. An application entitled "Optical Method And apparatus for the Diagnosis of Cervical Precancers using Raman and Fluorescence Spectroscopies" (Richards-Kortum et al . ) was filed on March 14, 1995, and is assigned to the same assignee as the present invention. Lui et al . , "Fluorescence and Time-Resolved Light Scattering as Optical Diagnostic Techniques to Separate Diseased and Normal Biomedical Media," J Photochem Photobiol B : Biol 16, 187-209, 1992. Wong et al . , "Infrared Spectroscopy of Human Cervical Cells: Evidence of Extensive Structural Changes during Carcinogenesis", Proc Nat 'l Acad Sci USA, 88, 10988-10992, 1991. Kurman RJ, Henson DE, Herbst AL, Noller KL, Shiffman MH, "Interim guidelines for management of abnormal cervical cytology," JAMA, 271, 1866-1869, 1994. Davison JM, Marty JJ, "Detecting premalignant cervical lesions: Contributions of screening colposcopy to cytology," J Reproductive Medicine, 39, 388-392, 1994 Banda-Gamboa, Hugo, et al, "Automation in cervical cytology: an overview," Anal Cell Path , 4 (1992) 25-48. Chance B, Thorell L, "Localization and kinetics of reduced PN in living cells by microfluorimetry," J " Biol Chem 234:3044, 1959. Caspersson TC, Cell Structure and Function , ch 4, Norton & Co, New York, 1950. Aubin JE, "Autofluorescence of Viable Cultured Mammalian Cells," J Histochem Cytochem, 27(1), 36-43, 1979. Benson RC, et al, "Cellular Autofluorescence: Is It Due to Flavins?" J Histochem Cytochem, 27(1), 44-48, 1979. Tamura M, ... Chance B, "In Vivo Study of Tissue Oxygen ...," Annu Rev Physiol , 1989, 51:813-814. Hutchinson, ML, Agarwal, P, Denault, T, Berger, B, Cibas, ES, "A New Look at Cervical Cytology," Acta Cytologica , 36, 4:499-504, 1992. Taylor DG, Demas JN, "Light intensity measurements I: Large area bolometers with uW sensitivities and absolute calibration of the rhodamine B quantum counter," Anal Chem, 51, 712-717, 1979. Vooijs GP, "Benign proliferative reactions, intraepithelial neoplasia and invasive cancer of the uterine cervix," in Bibbo M (ed) , Comprehensive Cytopathology, WB Saunders Company, pp 176-177, 1991. Dillon WR and Goldstein M, "Multivariate Analysis Methods and Applications," Chapter 10. Wiley & Sons, New York (1985)

Claims: CLAIMS ! 1. An optical probe for cervical examination comprising: (a) a probe casing having an elongate portion and at least two openings, wherein one of said openings is disposed at a distal tip of said elongate portion of said casing; (b) a plurality of optical fiber pairs, each of said pairs comprising an excitation fiber and a collection fiber, said optical fibers entering said casing at the non-tip opening and traversing said casing to said tip opening; and (c) a substantially optically transparent window with a surface structured to conform to the surface of the human cervix, said window covering said tip opening. 2. The probe according to claim 1, wherein at least a portion of said window is substantially conical. 3. The probe according to claim 1, wherein said probe casing is a hollow tube. 4. The probe according to claim 3, wherein said probe casing is comprised of carbonized epoxy. 5. The probe according to claim 3, wherein said probe casing is comprised of aluminum. 6. The probe according to claim 1, wherein said probe casing is further encased in heat-shrink tubing. 7. The probe according to claim 1, wherein individual fibers are comprised of a core and a cladding. 8. The probe according to claim 7, wherein said plurality is 31 or greater. 9. The probe according to claim 8, wherein said plurality is greater than 357 or greater. 10. The probe according to claim 1, wherein said window is comprised of quartz. 11. The probe according to claim l, wherein said window is comprised of BK7 glass. 12. The probe according to claim 1, wherein said tip is about 10 mm in width, having a minimum thickness of about 4 mm and a maximum thickness of about 7.6 mm. 13. The probe according to claim 2, wherein said window surface also comprises a non-conical portion. 14. The probe according to claim 13, wherein said tip is about 40 mm in width, having a minimum thickness of about 6.6 mm and a maximum thickness of about 10 mm, and said conical portion is about 10 mm in width. 15. The probe according to claim 1, wherein said tip further comprises a perforated plate. 16. The probe according to claim 15, wherein said plate is aluminum and said perforations are 500 μm in diameter and spaced every 2 mm. 17. The probe according to claim 1, wherein each of said fiber pairs are disposed in the center of glass tubes, said glass tubes having an inner diameter of 500 μm and an outer diameter of 2 mm. 18. An apparatus for measuring tissue fluorescence comprising (a) a probe according to claim 1; (b) an electromagnetic radiation generator; (c) a fluorescent spectrum detector; and (d) a means for analyzing said spectra in relation to said electromagnetic radiation. 19. The apparatus of claim 18, wherein said fiber pairs of said probe are connected to said fluorescent spectrum detector by a coupling means, said fiber pairs in said coupling means being arranged in a substantially linear fashion.

Inventors: MITCHELL MICHELE FOLLEN, PITRIS COSTAS, RICHARDS-KORTUM REBECCA, MITCHELL, MICHELE, FOLLEN, PITRIS, COSTAS, RICHARDS-KORTUM, REBECCA
Applicants: UNIV TEXAS, THE BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYST
Application year: 1996
Main classification: A61B5
IPC codes: A61B5, A61B10