NOVEL VINYL SULFIDE COMPOUNDS AND A PROCESS FOR THEIR PREPARATION

Abstract: Vinyl sulfides bearing protected arlyoxy substituents and a process for their preparation are provided. In the process a vinyl sulfide is formed by coupling a ketone compound and a thioester compound with a low valent titanium reagent to form both carbon-carbon bonds in the same step. The vinyl sulfide compounds are useful as intermediates which are convertible into triggerable sulfur-substituted dioxetanes by addition of oxygen to the double bond. The sulfur-substituted dioxetanes additionally are useful for producing chemiluminescence or for further conversion into triggerable alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy-substituted dioxetanes. Triggerable dioxetanes are useful for producing chemiluminescence and in detecting activating agents in assays such as immunoassays and nucleic acid hybridization assays.

Description: NOVEL VINYL SULFIDE COMPOUNDS AND A PROCESS FOR THEIR PREPARATION CROSS REFERENCE TO RELATED APPLICATION This application is a continuation-in-part of applicant's co-pending U.S. Application Serial No. 08/492,715 filed on June 20, 1995. BACKGROUND OF THE INVENTION (1) FIELD OF THE INVENTION The present invention relates to a novel process for the preparation of stable chemiluminescent 1,2-dioxetane compounds which can be triggered to generate light. Stable, triggerable dioxetanes prepared by the present process are preferably of the formula: The present invention also relates to novel sulfur- substituted alkenes (vinyl sulfides) preferably of the formula: R 2 R 3 ox and stable triggerable sulfur-substituted 1,2-dioxetanes preferably of the formula: a process for their preparation and a process for their use as intermediates for producing stable triggerable 1,2-dioxetanes substituted on the dioxetane ring with alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy groups. (2) DESCRIPTION OF RELATED ART a. Synthesis of Dioxetanes. The preparation of dioxetanes with alkoxy substituents by addition of singlet oxygen to a vinyl ether is well known in the art. Singlet oxygen is typically produced by irradiation of a photosensitizing dye in the presence of oxygen but can also be generated by thermolysis of triphenylphosphite ozonide. Other methods of preparing dioxetanes with alkoxy substituents from vinyl ethers include electron-transfer oxidation with oxygen and triarylaminium cation radical salts (R. Curci, L. Lopez, L. Troisi, S.M.K. Rashid and A.P. Schaap, Tetrahedron Lett . 28, 5319-22 (1987); L. Lopez, L. Troisi and G. Mele, Tetrahedron Lett . 32, 117-20 (1991)), oxidation by Cr(VI) or Mo(VI) oxide diperoxides (R. Curci, L. Lopez, L. Troisi, S.M.K. Rashid and A.P. Schaap, Tetrahedron Lett . 29, 3145-8 (1988)) and oxidation with triethylsilyl hydrotrioxide (G.H. Posner, K.S. Webb, W.M. Nelson, T. Kishimoto and H.H. Seliger, J " . Org. Chem. , 54, 3252-4 (1989)). A dioxetane was produced in low yield by reaction of a dioxene compound with oxygen which had been passed through an electric discharge, apparently producing a small amount of singlet oxygen in addition to ozone (T.-S. Fang and W.-P. Mei, Tetrahedron Lett . 28, 329-21 (1987)). All of these methods for the preparation of alkoxy-substituted dioxetanes require the preparation of the precursor vinyl ether. No reaction involving the direct introduction of alkoxy or aryloxy groups on a pre-formed dioxetane ring has been reported to the best of applicant's knowledge. There is thus a need for a general method for the preparation of a variety of alkoxy-substituted dioxetanes from a common intermediate which does not require the preparation of each individual vinyl ether precursor. b. Sulfur-Substituted Dioxetanes. 1,2-Dioxetanes with one or more sulfur-containing substituents on the dioxetane ring are known. All known examples are unstable, with most decomposing rapidly at room temperature. (W. Adam, L. A. Arias, D. Scheutzow, Tetrahedron Lett . , 23(28), 2835-6 (1982); W. Adam, L. A. Encarnacion, Chem. Ber. , 115(7), 2592-605 (1982); W. Ando, K. Watanabe, T. Migita, J. Chem. Soc , Chem. Commun . (24), 961-2 (1975); G. Geller, CS. Foote, D.B. Pechmann, Tetrahedron Lett . 673-6 (1983); R.S. Handley, A. J. Stern, A.P. Schaap, Tetrahedron Lett . 26, 3183-6 (1985)) . The most stable sulfur-substituted dioxetanes, derived from 4,5-dialkyl-2,3-dihydrothiophene decompose with a half-life of a few minutes at room temperature (W. Adam, A. Griesbeck, K. Gollnick, K. Knutzen-Mies, J " . Org. Chem. , 53, 1492-5 (1988); K. Gollnick, K. Knutzen-Mies, J. Org. Chem. , 56, 4027-31 (1991)). Two spiroadamantyl-substituted dioxetanes bearing one and two sulfur substituents, respectively, on the dioxetane ring are known. Both have been reported to rapidly and completely decompose on attempted isolation at room temperature (W. Adam, L. A. Encarnacion, Chem. Ber. , 115(7), 2592-605 (1982)). c. Synthesis of Vinvl Sulfides. Vinyl sulfides containing a carbon-carbon double bond and a sulfur substituent directly attached to one of the double bond carbon atoms can be prepared by various methods known to the skilled synthetic chemist. One of the classical methods for preparation of vinyl sulfides involves the reaction of a ketone and mercaptan with TiCl 4 and an amine base (T. Mukaiyama, K. Saigo, Chem. Lett., 479-82, 1973)). Vinyl sulfides have been formed by other methods which utilize titanium reagents. Vinyl sulfones are reduced at the sulfur to vinyl sulfides with LiAlH 4 -TiCl 4 (E. Akgun, K. Mahmood, CA. Mathis, J. Chem . Soc , Chem . Commun, (6), 761-2 (1994)). α-Halo-sulfoxides undergo elimination and reduction with Zn-TiCl 4 to form vinyl sulfides (V. Retrakul, P. Poochaivatananon, Tetrahedron Lett . , 24(5), 531-4 (1983)). In each of these methods, one or both of the C-C bonds is already formed in the starting material. None of the foregoing methods involves the direct formation of the sulfur-substituted carbon-carbon double bond from two separate carbon atoms. No method of which Applicants are aware is known for creating a vinyl sulfide by coupling two carbonyl-containing compounds, one of which is a thioester, to form a double bond with a sulfur-substituent. d. Chemically Triσσerable Dioxetanes. Chemically triggerable adamantyl-stabilized dioxetanes are disclosed in U.S. Patent 4,857,652 and a paper (A. P. Schaap, T. S. Chen, R. S. Handley, R. DeSilva, and B. P. Giri, Tetrahedron Lett . , 1155 (1987)). These dioxetanes exhibit thermal half-lives of years but can be triggered to produce efficient chemiluminescence on demand. Benzofuranyl dioxetanes substituted with trialkylsilyl and acetyl-protected phenolic groups which produce weak chemiluminescence have also been reported (W. Adam, R. Fell, M.H. Schulz, Tetrahedron, 49(11), 2227-38 (1993); W. Adam, M.H. Schulz, Chem. Ber. , 125, 2455-61 (1992)) . Each of these dioxetanes was prepared by dye-sensitized photooxygenation of a vinyl ether (alkene) precursor. e. Enzvmaticallv Triσσerable Dioxetanes. Enzymatic triggering of adamantyl-stabilized 1,2-dioxetanes are described in U.S. Patent 4,857,652 and a series of papers (A. P. Schaap, R. S. Handley, and B. P. Giri, Tetrahedron Lett., 935 (1987); A. P. Schaap, M. D. Sandison, and R. S. Handley, Tetrahedron Lett . , 1159 (1987) and A. P. Schaap, Photochem. Photobiol . , 47S. 50S (1988)) . These dioxetanes bear a protected aryloxide substituent which is triggered to decompose with emission of light by the action of an enzyme in an alkaline aqueous buffer to give an aryloxide intermediate dioxetane which decomposes with emission of light at a greatly increased rate. Chemiluminescence is thereby emitted at a much greater intensity than that resulting from slow thermal decomposition of the protected form of the dioxetane. Further examples of enzymatically triggered dioxetanes are disclosed in U.S. Patent No. 5,068,339 to Schaap, U.S. Patent Nos. 5,112,960 and 5,220,005 and a PCT application (88 00695) to Bronstein , U.S. Patent No. 4,952,707 to Edwards, U.S. Patent 5,132,204 to Urdea, U.S. Patent 5,248,618 to Haces and PCT application WO94/10258 to Wang and in a publication (M. Ryan, J.C Huang, O.H. Griffith, J.F. Keana, J.J. Volwerk, Anal . Biochem. , 214(2), 548-56 (1993)). The enzymatically triggerable dioxetanes are now undergoing widespread use as substrates for marker enzymes in numerous applications including immunoassays, gene expression studies, Western blotting, Southern blotting, DNA sequencing and the identification of nucleic acid segments in infectious agents. New processes for the preparation of existing and new triggerable dioxetanes are desirable to advance the state of the art. Processes which permit the preparation of dioxetane compounds which are difficult or impossible to prepare by known methods would be particularly desirable. The process of the present invention provides such means. OBJECTS It is an object of the present invention to provide novel aryl group-substituted 1,2-dioxetanes further substituted on the dioxetane ring with a thioalkyl or thioaryl group SR 4 . It is an object of the present invention to provide novel aryl group-substituted vinyl sulfides and a process for their preparation, said vinyl sulfides being used in preparing the sulfur-substituted dioxetanes. It is a further object of the present invention to provide a process for producing stable 1,2-dioxetanes by replacement of the sulfur-containing group SR 4 of a sulfur-substituted dioxetane with an alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy group OR 5 . It is also an object of the present invention to provide a process of preparation of stable 1,2-dioxetanes containing an alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy group OR 5 and further substituted on the dioxetane ring with aryl group substituted with an OX substituent which can be triggered by activating agents to remove a protecting group X and consequently generate chemiluminescence. It is a further object of the present invention to provide a general process for producing a number of different stable 1,2-dioxetanes each substituted on the dioxetane ring with different alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy groups OR 5 by replacement of the sulfur-containing group SR 4 of a sulfur-substituted dioxetane. Dioxetane compounds prepared by the process of the present invention can be used in assay methods to signal the presence or amount of an analyte, in emergency and novelty lighting applications and as light standards for luminometer calibration. Dioxetane compounds which are triggered by an activating agent to produce light are useful in immunoassays and the detection of nucleic acids, antibodies, haptens and antigens by generally known methods. DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention relates to a general process for the preparation of stable aryl group-substituted 1,2-dioxetanes (III) further substituted on the dioxetane ring with an alkoxy, aryloxy or acyloxy group which dioxetane can be triggered to generate chemiluminescence. The process involves the substitution of an alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy group OR 5 for a thioalkyl or thioaryl group SR 4 on the dioxetane ring of a sulfur-substituted dioxetane (II) mediated by an electrophilic compound E-Y. Until Applicant's process was discovered, no process existed which was generally applicable to the preparation of a series of stable dioxetanes from a common intermediate; each dioxetane was prepared by addition of oxygen to the independently prepared alkene precursor. The success of the present process resides in the unanticipated discovery that certain sulfur-substituted dioxetanes can react with hydroxyl group-containing compounds in the presence of an electrophile to replace the sulfur substituent with an alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy group OR 5 . Sulfur-substituted dioxetanes of the present invention are prepared by addition of oxygen to a vinyl sulfide (I) . The sequence of reactions is shown in the Scheme below. Scheme 1, II II III While not wishing to be bound by any particular theory, a plausible explanation for the reaction is illustrated in the Scheme shown below. Scheme 2 R 5 -OH No reaction involving the direct substitution of groups on a dioxetane ring has ever been reported. The present invention represents the first demonstration of the synthesis of a dioxetane by replacing one of the dioxetane ring substituents of a different precursor dioxetane. The ability to selectively replace one of the ring substituents readily allows the synthesis of a variety of new dioxetane compounds from a common intermediate. Sulfur-substituted dioxetanes useful in practicing the process of the present invention can be of the formula: wherein R x and R 2 are organic groups providing sufficient stability to the dioxetane to permit conversion to an alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy-substituted dioxetane. The groups R τ and R 2 are chosen from branched chain or cyclic alkyl, substituted alkyl or heteroalkyl groups. R x and R 2 can optionally be joined together to form a cyclic or polycyclic group which is spiro-f sed to the dioxetane ring. The group R 4 is selected from (C 1 -C 20 ) alkyl, (C 7 -C 30 ) aralkyl and (C 6 -C 30 ) aryl groups which can optionally contain non-interfering substituents and optionally contain N, 0, S, P or halogen heteroatoms within the alkyl, aralkyl or aryl group. The group R 3 is selected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl or heteroaryl groups in which one or more of the ring hydrogens can be replaced by an atom or group selected from halogens, alkyl, alkoxy, substituted alkoxy, carbonyl, carboxyl, amino and alkylamino groups. The X group can be any protecting group which serves to block formation of the aryloxide anion and which can be replaced or removed as desired by an activating agent to form the aryloxide anion. Representative OX groups include hydroxyl, alkoxy, substituted alkoxy (e.g. methoxyethoxy- methoxy (MEM-O) and trimethylsilylethoxymethoxy (SEM-O) ) , acyloxy having the formula OOCR 10 wherein R 10 is selected from alkyl and aryl groups containing 2 to 20 carbon atoms, trialkylsilyloxy, triarylsilyloxy, aryldialkylsilyloxy, OPO(OR 8 ) 2 wherein R 8 is an organic group, oxygen pyranoside including, without limitation, β-D-galactosyloxy and β-D-glucuronidyloxy groups. In a preferred embodiment, the process is used to form a sulfur-substituted dioxetane (V) wherein the R. and R 2 groups are joined together as a polycyclic alkyl group spiro-fused to the dioxetane ring as represented by the formula: ." •• V wherein L C is the polycyclic alkyl group. Most preferred is a sulfur-substituted dioxetane wherein the R 6 C group is an adamantyl group with optional non-hydrogen substituents. Electrophilic agents useful in practicing the present invention include but are not limited to halogens including Cl 2 , Br 2 , l 2 , ICI and IBr, hydrogen peroxide, singlet oxygen, pseudo-halogens such as N-chlorosuccinimide (NCS) , N-bromosuccinimide (NBS) and N-iodosuccinimide (NIS) , alkylating agents including alkyl halides and alkyl sulfates and sulfonates, transition metal salts, especially salts of mercury, silver and gold, and Lewis acids such as titanium tetrachloride. In one embodiment, the compound of structure E-Y may serve as both the electrophilic agent and the hydroxylic compound R 5 0"M + . Compounds of this type described generically as (R 5 0 " ) n M +n wherein M is a metal with a strong propensity to react with sulfur, such as silver, gold and especially, mercury, wherein n is 1,2 or 3 and wherein R 5 0 ~ is an anion of a hydroxylic compound, in particular a carboxylate anion are effective to replace the SR 4 group of a sulfur-substituted dioxetane with an OR 5 group. A preferred compound of this type is mercuric acetate Hg(OAc) 2 . Photosensitizers useful in practicing the process of the invention include compounds known in the art to produce singlet oxygen upon irradiation with visible light in the presence of ground state oxygen. Exemplary photosensitizers include methylene blue, Rose Bengal, eosin, erythrosin, rhodamines, porphyrins, metal porphyrins and fullerenes. Photosensitizers may be employed as the soluble dye or linked to an insoluble support such as silica or a polymer bead. Preferred photosensitizers are methylene blue or polymer-bound Rose Bengal. In a preferred mode of carrying out the inventive process, a vinyl sulfide containing a sulfur substituent SR 4 , wherein R 4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms is converted by low temperature photooxygenation to the corresponding sulfur- substituted 1,2-dioxetane by addition of singlet oxygen to the double bond. Progress of this reaction is readily monitored by thin layer chromatography (TLC) or 1 H NMR by observing the disappearance of the vinyl sulfide. Additionally, heating a small portion of the reaction solution leads to easily detectable chemiluminescence indicating formation of the sulfur-substituted dioxetane. Visualization of the sulfur-substituted dioxetane by triggering with fluoride in DMSO produces yellow-greenish chemiluminescence which is discernible to the unaided eye. Sulfur-substituted dioxetane compounds of relatively lower thermal stability are preferably not isolated at this point but instead directly reacted at a low temperature with a compound containing a hydroxyl group or a salt thereof. Sulfur-substituted dioxetane compounds of relatively higher thermal stability can be first isolated before reaction with a compound containing a hydroxyl group or a salt thereof. An electrophilic compound is added at low temperature to the sulfur-substituted dioxetane in an amount ranging from about 0.5 mol to about 1.5 mol of electrophilic compound per mol of dioxetane based on complete conversion of the vinyl sulfide. It is especially preferred that the photooxygenation and addition of electrophilic compound be performed at or below about -40 °C; use of a Dry Ice-isopropyl alcohol mixture, ca. -78 °C, for cooling is particularly suited for this purpose. A hydroxylic compound R 5 -OH selected from alcohols, phenols where phenols are defined as aryl ring compounds with at least one OH substituent and carboxylic acids or their salts, if not already present as the reaction solvent, is added at low temperature in an amount of at least about 1 mol to 2 mol per mol of the vinyl sulfide. The hydroxylic compound is permitted to react with the dioxetane to effect replacement of the SR 4 group with the OR 5 group. Vinyl sulfide compounds (I) useful in practicing the present invention are preferably of the formula: I wherein R and R 2 are organic groups which can optionally be joined together to form cyclic or polycyclic groups, wherein R 4 is selected from (C^C- j o) alkyl, (C 7 -C 30 ) aralkyl and (C 6 -C 30 ) aryl groups and optionally containing heteroatoms, wherein R 3 is selected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl or heteroaryl groups which can optionally contain non-interfering substituents and wherein X is a protecting group. Preferred R. and R 2 groups are selected from branched chain alkyl, cycloalkyl and aryl groups containing 3 to 20 carbon atoms and optionally heteroatoms. In a preferred embodiment, a vinyl sulfide having the formula: ✓"""N .,S-R 4 e C=< ^-' R 3 OX (IV) _•*•**» is used wherein R 6 C s selected from cyclic and polycyclic >*.»•< organic groups which can optionally be substituted. It is preferred that R 3 is an optionally substituted phenyl, naphthyl or other fused-ring aryl group. It is especially preferred that R 3 is a phenyl group in which an OX group is oriented meta to the dioxetane ring group as shown below. The phenyl ring can optionally contain additional ring substituents independently selected from halogens, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carbonyl, carboxyl, amino and alkylamino groups. The group R. C is more preferably a polycyclic group, preferably an adamantyl group optionally having one or more non-interfering substituent groups selected from halogens, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carbonyl, carboxyl, phenyl, substituted phenyl, amino and alkylamino groups covalently bonded thereto. Vinyl sulfides useful in practicing the process of the present invention can be prepared by art-known methods for the preparation of vinyl sulfides. An exemplary procesε for the preparation of vinyl sulfides is disclosed in T. Mukaiyama, K, Saigo, Chem. Lett., 479-82 (1973). In another embodiment of the present invention, vinyl sulfide (IV) is formed by coupling a ketone compound and a thioester compound with a low valent titanium reagent exemplified by the following reaction: Scheme 3. _. c=o This reaction process achieves the direct formation of both carbon-carbon bonds of the vinyl sulfide product. The low valent titanium reagent, which is used in excess, is prepared by reacting a titanium salt, preferably TiCl 3 or TiCl 4 with a metallic reducing agent selected from lithium, sodium, potassium, zinc and zinc-copper alloys or a metal hydride including, without limitation, sodium hydride, potassium hydride, aluminum hydride, lithium aluminum hydride or potassium triethylborohydride in the presence or absence of an amine base in an aprotic solvent, preferably tetrahydrofuran. The ratio of ketone to thioester is preferably from about 1:3 to about 3:1. The reaction between the ketone and thioester is conducted between about 0 °C and the reflux temperature of the solvent, preferably between about 20 °C and about 70 °C Thioalkyl- and thioaryl-substituted dioxetanes of the present invention are surprisingly stable and can be manipulated at temperatures of -10 to 25 °C Reaction of the thioalkyl- and thioaryl-substituted dioxetanes of the present invention with an activating agent to cleave the O-X bond and remove the X group yields an unstable oxide intermediate dioxetane compound is formed which decomposes and releases electronic energy to form light and two carbonyl-containing compounds of the formula: In contrast, all dioxetanes bearing sulfur substituents known in the prior art including those with an adamantyl substituent, spontaneously decompose at or, in most cases, well below room temperature. It was particularly unexpected that a sulfur-substituted dioxetane would exhibit decay rates in alkaline amine buffers containing metal saltε comparable to analogous alkoxy-substituted dioxetanes. Stable alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy-substituted dioxetanes which can be prepared by the process of the present invention are of the formula: wherein R x and R 2 are organic groups which provide stability and which can optionally be joined together to form a cyclic or polycyclic group which is spiro-fused to the dioxetane ring, wherein R 5 is selected independently from the group consisting of (C 1 -C 20 ) alkyl groups, (C 2 -C 20 ) alkenyl groups, (C 2 -C 20 ) alkynyl groups, (C 6 -C 30 ) aryl groups, (C 7 -C 30 ) aralkyl groups and (C 1 -C 20 ) acyl groups. R 5 can optionally be substituted with one or more substituents including, without limitation, hydroxy, alkoxy, halogen, cyano, nitro, amino, imine, ketone, aldehyde, carboxylic acid, carboxylic ester, carboxamide, thiol, thioester, trialkylεilyloxy, triarylsilyloxy and alkyldiarylsilyloxy substituents. The group R 3 is selected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl and fused ring polycyclic heteroaryl groups which can optionally be substituted with non-interfering groups. X is a group which can be removed by an activating agent as is generally known in the art to form an unstable oxide intermediate dioxetane compound. In another embodiment, X can be a group such as a hydrogen atom or a labile group which can be replaced with another removable group without triggering the decomposition of the dioxetane. Representative OX groups include hydroxyl, alkoxy, substituted alkoxy (e.g. methoxyethoxymethoxy (MEM-O) and trimethylsilylethoxy- methoxy (SEM-O) ) , acyloxy having the formula OOCR 10 wherein R 10 is selected from alkyl and aryl groups containing 2 to 20 carbon atoms, trialkylsilyloxy, triarylsilyloxy, aryldialkylsilyloxy, OPO(OR 8 ) 2 wherein R 8 is an organic group, OP0 3 2~ salts and oxygen pyranoside including, without limitation, β-D-galactosyloxy and β-D-glucuronidyloxy groupε. When chemiluminescence is to be generated the stable dioxetane is triggered with an activating agent to generate an unstable oxide intermediate dioxetane which decomposes and releases electronic energy to form light and two carbonyl containing compounds as εhown in Scheme 4 below. Scheme 4. A preferred dioxetane compound which can be made by the process of the present invention is a stable dioxetane of the formula: wherein R 6 C is selected from cyclic and polycyclic organic groups, which can optionally be substituted with non¬ interfering groups and which is spiro-fused to the dioxetane ring and which provides thermal stability, wherein R 3 , R 5 and X are as defined above so that when the dioxetane is triggered to remove the X group by an activating agent an unstable oxide intermediate dioxetane compound is formed which decomposes and releases electronic energy to form light and two carbony1-containing compounds of the formula: In a preferred embodiment, the group R^C is a polycyclic organic group spiro-fused to the dioxetane ring, containing 6 to 30 carbon atoms and which can optionally be substituted with non-interfering groups and which provides thermal stability. The group R^ C is more preferably an adamantyl group optionally having at least one substituent group selected from halogens, alkyl, substituted alkyl, alkoxy, substituted alkoxy, carbonyl, carboxyl, phenyl, substituted phenyl, amino and alkylamino groups covalently bonded thereto. In another preferred embodiment the group R 3 is a phenyl or naphthyl group. It is especially preferred that R 3 is a phenyl group in which the OX group as defined above is oriented meta to the dioxetane ring group as shown below. The phenyl ring can optionally contain additional ring subεtituents independently selected from halogens, alkyl, substituted alkyl, alkoxy, εubεtituted alkoxy, carbonyl, carboxyl, amino and alkylamino groups. ox The stable 1,2-dioxetane compounds have long half-lives at room temperature, typically > 1 year, but can be triggered by an activating agent to decompose rapidly with half-lives ranging from seconds to a few minutes depending on the microenvironment where the dioxetane is located. Aryloxy-substituted dioxetanes of the formula: have been prepared, it is believed for the first time, by the process of the present invention by using a phenol as the hydroxylic compound R 5 OH. Dioxetanes wherein Ar is an optionally substituted (C 6 -C 30 ) aryl group and wherein X is a removable group are highly stable compounds which produce visible light when triggered to remove X. It is contemplated that the hydroxylic compound R 5 OH may itself be another dioxetane molecule with a phenolic group. In this embodiment, chainε of directly linked, oligomeric or polymeric dioxetaneε are formed. Further, such oligomeric or polymeric dioxetanes can serve as the hydroxylic compound in order to build chain structures of greater length. A requirement for this proceεs is that the OX group of the monomeric, oligomeric or polymeric dioxetane serving as the R' 5 OH component be present aε OH. An example εhowing formation of a dimeric biε-dioxetane (VII) is illustrated below to aid in underεtanding the proceεs. Scheme 5. It will be obvious in view of the foregoing description that, under appropriate conditions, the intermediate carbonium ion can serve as its own R 5 OH component; i.e. when X is H a self-condensation can occur to produce an oligomeric or polymeric dioxetane chain compound. A sub¬ stoichiometric amount of a competing R' 5 OH compound is required to effect chain termination. A poεεible dioxetane compound (VIII) prepared by thiε proceεε iε illuεtrated to aid in underεtanding the proceεε. An acyloxy-substituted dioxetane of the formula: has been prepared for the firεt time by the preεent proceεε. In preparing a dioxetane of thiε εtructure, the hydroxylic compound can be a carboxylic acid R 9 COOH, a εalt thereof or it can be aε the electrophilic compound in the manner described above. Dioxetanes wherein R g is an optionally subεtituted (C^C*^) alkyl or aryl group and wherein X is a removable group are also stable compounds which emit light when triggered to remove X. A significant advantage of the present procesε for preparing alkoxy, alkenyloxy, alkynyloxy, aryloxy, aralkyloxy or acyloxy-εubεtituted dioxetanes is that a single vinyl sulfide precursor compound can be converted into any of a large number of other dioxetane compounds limited only by the availability of the hydroxylic compound R 5 -OH or εalt R 5 0 _ M + . Since the OR 5 group iε not introduced until after formation of the dioxetane ring, it is possible to prepare dioxetane compounds which would be problematic to prepare by the prior art process of photooxygenating a vinyl ether precursor. The present process is amenable to the preparation of dioxetanes with groups which could interfere with the photooxygenation either by quenching of singlet oxygen, or by quenching or bleaching the photosensitizer. The present procesε iε alεo amenable to the preparation of dioxetanes with groups which make the vinyl ether more electron-deficient and therefore less reactive or unreactive to singlet oxygen. The present process further permits the preparation of dioxetanes with groups such as C-C double bonds which are themselves reactive toward singlet oxygen. Dioxetanes prepared by the procesε of the preεent invention can be uεed in a process for generating light which comprises reacting an activating agent with a stable 1,2-dioxetane of the formula (III): wherein the X group iε removed by the activating agent to form an unstable oxide intermediate dioxetane compound which decomposes and releaseε electronic energy to form light and two carbonyl-containing compoundε of the formula: In another embodiment, the activating agent can be a chemical, including an enzyme, which reacts catalytically or stoichiometrically to trigger the dioxetane. Exemplary activating agentε are disclosed, for example, in U.S. Patent 4,857,652 the relevant portion of the disclosure of which is incorporated herein by reference. Activating agents which react catalytically or stoichiometrically to trigger the dioxetane by removal of the X group are well known in the art. Reactions can be conducted in aqueous solution, in which case it is often desirable to use one or more art-known chemiluminescence enhancers to increase or prolong light emission. Reactions can also be conducted in polar, aprotic solventε εuch as dimethyl sulfoxide, N,N- dimethylformamide or acetonitrile. The procesε for generating light can be performed in εolution or on the εurface of a solid support such as a membrane. Dioxetanes prepared by the procesε of the present invention can further be incorporated into a chemilumin¬ escent composition which comprises a dioxetane, one or more enhancer substances and optional fluorescers. Enhancers are subεtanceε which increase the amount of light produced on triggering the dioxetane. Enhancer εubstances well known in the art increase chemiluminescence either by providing a hydrophobic environment in which the light emitting reaction can occur or through energy transfer to a fluorescent compound held in proximity to the dioxetane. The development of particular asεayε and kitε uεing dioxetanes prepared by the procesε of the present invention will be readily apparent to the skilled artisan. The methodε of use of the dioxetanes which can be prepared by the present process are illustrative of their utility only. The uses do not conεtitute a part of the invention per εe. EXAMPLES In order to more fully describe the various aspects of the present invention, the following examples are described. The examples are to be considered illustrative and do not limit the scope of the invention. Sulfur-subεtituted dioxetane compoundε prepared uεing the process of the present invention are shown below. Table 1. Dioxetane Compounds Dioxetane — & 4 _ OX 1 CH 2 CH 3 OH 2 CH 2 CH 3 OOCC ( CH 3 ) 3 3 4-C 6 H 4 F OH 4 4-C 6 H 4 F OOCC (CH 3 ) 3 5 CH 2 CF 3 OH Examole 1 . Synthesis Pf Comnound 1 . 4 • -Ethylthio-4 hydroxyphenyl) spiro[1, 2-dioxetane-3 ,2 ' -tricyclo [3.3.l.l 3 - 7 ] decane] . (a) Synthesiε of 3-hydroxyphenyl tricyclo [3.3.1.1 3 - 7 ] dec-2- yl ketone. To a εtirred εolution of 20.00 g (0.07397 mol) of [ (3-hydroxyphenyl)methoxymethylene] tricyclo[3.3.l.l 3 - 7 ] - decane (preparation described in U.S. Patent 4,857,652) in 300 mL of methanol was added 5 mL of concentrated hydrochloric acid. After about fifteen minutes, a white precipitate began to form. The reaction was allowed to stir overnight. Water (500 mL) was added to the reaction mixture, and the precipitate was collected by suction filtration and washed with an additional 500 mL of water. After air drying for a few hourε, the solid was dissolved in ethyl acetate and dried over magnesium sulfate. The magnesium sulfate waε filtered and the ethyl acetate removed in vacuo yielding 23.42 g (97% yield) of the pure ketone as a white solid. l H NMR (CDC1 3 ) δ 1.55-2.05 (m, 12H) , 2.309 (br s, 2H) , 3.41 (br ε, IH) , 6.99-7.39 (m, 4H) . (b) Synthesis of [ (3-hydroxyphenyl) ethylthiomethylene] tricyclo[3.3.1. I 3 - 7 ]decane. The ketone was converted to the vinyl sulfide by the method of Mukaiyama (T. Mukaiyama, K, Saigo, Chem. Lett., 479-82, 1973)) . An argon-purged round bottom flask was charged with 10.0 g (39 mmol) of the hydroxy ketone, 250 mL of anhydrous tetrahydrofuran (THF) , and 9.5 mL (2.2 eq. ) of TiCl4. The resulting brown solution was εtirred under argon for 30 minuteε at room temperature. A εolution of 24.0 mL (4.4 eq. ) of triethylamine (Et3N) dried over KOH and 3.2 mL (1.1 eq. ) of ethanethiol in 200 mL of dry THF waε then added dropwiεe to the reaction εolution over a 2 hour period while the reaction εolution darkened to a deep reddiεh brown. Stirring waε maintained overnight. A portion of dry Et3N (100 mL) waε added to the reaction εolution cauεing the brief formation of εome precipitate. The exceεε TiCl4 waε neutralized by the addition of methanol. Thiε cauεed the formation of precipitate, turned the reaction mixture from brown to yellow to green, and caused the reaction mixture to heat up. The reaction mixture was found to still be basic by pH paper and waε allowed to εtir for an hour. The reaction mixture had turned back to a bright yellow color by the end of this hour. The mixture was then suction filtered and the white precipitate obtained was washed with ethyl acetate and discarded. The solvents were then removed in vacuo from the filtrate yielding a brownish yellow solid. This solid was then taken up into 500 mL of ethyl acetate and the resulting mixture washed several times with water (emulsion formed) . The ethyl acetate layer was then dried over magnesium sulfate, filtered and concentrated in vacuo yielding a viscous yellow oil which smelled of thiol. The oil was chromatographed on silica gel using methylene chloride as eluent. The deεired vinyl εulfide and the starting ketone were recovered. The solvents were removed in vacuo from the first eluted fraction yielding 8.57 g of light yellow oil which gradually solidified over the course of several days. 1 H NMR (CDC1 3 ) δ 1.105 (t, 3H, J=7.3 Hz), 1.64-2.00 (m, 12H) , 2.238 (q, 2H, J=7.3 Hz), 2.64 (br s, IH) , 3.58 (br s, IH) , 4.813 (ε, IH) , 6.69-6.83 (m, 3H) , 7.175 (t, IH) . (c) Syntheεis of 4-ethylthio-4- (3-hydroxyphenyl)spiro[1,2- dioxetane-3,2 ' -tricyclo[3.3.1.I 3 - 7 ]decane] (1) . A small photooxygenation apparatus was charged with 102 mg of the vinyl sulfide, 1-2 mg of methylene blue, and 10 mL of methylene chloride dried over magneεium εulfate. The reεulting εolution waε then cooled to -78 °C with oxygen bubbling through it. After εeveral minuteε, the reaction solution was irradiated with a 1000 W sodium lamp (GE LUCALOX) εhielded by a 0.005" polyimide film (DuPont) for 20 min to produce the dioxetane. Mixing a sample of the dioxetane with an excess of tetrabutylammonium fluoride (TBAF) in DMSO produced yellow chemiluminescence. Rxamnle 2. Synthesis of Compound 2. 4-Ethylthio-4- (3- pivaloyloxypheny1) spiro[1,2-dioxetane-3 ,2 ' -tricyclo- [3.3.1.1 3 < 7 ]decane] . (a) Synthesiε of 3-pivaloyloxyphenyl-tricyclo[3.3.l.l 3 ' 7 ] - dec-2-yl ketone. 3-Hydroxyphenyl-tricyclo[3.3.1. l 3 - 7 ]dec-2- yl ketone (2.1 g, 8.2 mmol) was dissolved in 200 mL of CH2CI2 and 4.6 mL of Et3N (4 eq. ) . Pivaloyl chloride (1.1 mL, 1.1 eq.) was added dropwise to the solution and the reaction stirred for two hours to complete acylation of the phenol group. The solution was extracted with three 100 mL portions of water and dried over MgS04. Evaporation of the solvent left a colorless oil which slowly solidified. Yield 2.77 g; i H NMR (CDC1 3 ) δ 1.369 (s, 9H) , 1.55-2.05 (m, 12H) , 2.307 (br s, 2H) , 3.422 (br s, IH) , 7.20-7.68 (m, 4H) . (b) Synthesis of [ (3-pivaloyloxyphenyl) ethylthiomethylene] - tricyclo[3.3.1. I 3 - 7 ]decane. An Ar-purged round bottom flask was charged with 0.50 g (1.47 mmol) of the pivaloyloxy ketone, 20 mL of dry THF, and 0.16 mL (1 eq.) of TiCl4. The resulting orange solution was treated with a solution of 0.43 mL (2.1 eq. ) of dry Et3N and 0.12 mL (1.1 eq. ) of ethanethiol in 20 mL of dry THF added dropwise to the reaction solution over a 2 hour period. The reaction solution darkened to a deep reddiεh brown. Stirring waε maintained for about 65 hourε. The exceεε TiCl4 waε neutralized by the addition of 5 mL of methanol. The solvents were then removed in vacuo yielding a yellow oil. The oil was chromatographed on silica gel using 5-20% ethyl acetate in hexane as eluent yielding 0.21 g of a colorless oil: l H NMR (CDCI 3 ) δl.096 (t, 3H, J=7.2 Hz), 1.355 (s, 9H) , 1.68-1.97 (m, 12H) , 2.238 (q, 2H, J=7.2 Hz), 2.66 (br s, IH) , 3.59 (br s, IH) , 6.91-7.32 (m, 4H) ; 13 C NMR (CDC1 3 ) 515.11, 25.89, 27.13, 28.04, 30.29, 35.48, 35.57, 36.96, 39.03, 39.36, 119.59, 120.41, 122.50, 126.66, 128.57, 141.44, 150.91, 153.22, 176.86. (c) Synthesis of 4-ethylthio-4- (3-pivaloyloxyphenyl)spiro- [1,2-dioxetane-3,2 ' -tricyclo[3.3.1.I 3 - 7 ] ecane] {_) . A small photooxygenation apparatus was charged with 60 mg (0.16 mmol) of the vinyl sulfide, 180 mg of polymer-bound Rose Bengal (U.S. Patent 4,315,998), and 20 mL of CH 2 C1 2 dried over MgS04. The resulting mixture was then cooled to -78 °C with oxygen bubbling through it. After several minutes, the reaction solution was irradiated with a 1000 W εodium lamp for 25 min. TLC using 10% ethyl acetate in hexane showed conversion to a new material which emitted light when heated. The sensitizer was filtered off, the solvent evaporated at 0 °C and the material examined by 1 H NMR. TLC suggested a higher degree of decomposition than was evident from the NMR spectrum suggesting that the dioxetane is less stable on silica. Mixing a sample of the dioxetane with an excess of TBAF in DMSO produced yellow chemiluminescence. ! H NMR (CDC1 3 ) δl.117 (t, 3H, J=7.2 Hz), 1.20-2.1 (m, 21H) , 2.21 (br s, IH) , 2.27-2.40 (m, 2H) , 3.11 (br s, IH) , 7.04- 7.60 (m, 4H) . Example . Synthesis of Compound . 4- (p-Fluorophenylthio) - 4- (3-hydroxyphenyl) εpiro[l,2-dioxetane-3 , 2 ' -tricyclo- [3.3.1.1 3 - 7 ]decane] . (a) Syntheεiε of [ (3-hydroxyphenyl) - (p-fluorophenylthio) - methylene] tricyclo[3.3.l.l 3 - 7 ]decane. A εolution of 10.33 g (40.3 mmol) of the hydroxy ketone of Example Ka) and 250 mL of dry THF waε purged with argon and treated with 9.8 mL (2.2 eq.) of TiCl4. After 30 min, the resulting brown solution was treated with a solution of 25.0 mL (4.4 eq.) of dry Et3N and 4.8 mL (1.1 eq. ) of 4-fluorothiophenol in 200 mL of dry THF added dropwise to the reaction solution over a 2 hour period. The reaction εolution darkened to a deep reddiεh brown while εtirred over night. Dry Et3N (100 mL) was added, changing the color to yellow. The excess TiCl4 was . neutralized by the addition of 2 mL of ethanol. The solventε were then removed in vacuo from the filtrate yielding a browniεh yellow εolid. Thiε solid was then taken up into 500 mL of ethyl acetate and the resulting mixture washed several times with water (emulsion formed) . The ethyl acetate layer was then dried over magnesium εulfate, filtered and concentrated in vacuo yielding a viscous yellow oil which smelled of the thiophenol. The oil was chromatographed on silica gel using methylene chloride as eluent. The vinyl sulfide was collected in two fractions, the first being contaminated with the thiophenol, the second being pure product. The first fraction was chromatographed on silica gel using 10-20% ethyl acetate in hexane as eluent yielding a second crop of vinyl sulfide which combined with the previous crop yielded 11.7 g: *H NMR (CDCl 3 ) δl.75-2.05 (m, 12H) , 2.783 (br s, IH) , 3.674 (br s, IH) , 4.559 (s, IH) , 6.57-7.118 (m, 8H) . (b) Synthesis of 4- (p-Fluorophenylthio) -4- (3-hydroxy- phenyl) spiro[1,2-dioxetane-3 , 2 ' -tricyclo[3.3.1.1 3 - 7 ] - decane] ) . A small photooxygenation apparatus waε charged with 54 mg of the vinyl εulfide, 1-2 mg of methylene blue, 5 mL of CH 2 C1 2 (dried over MgSθ4) and 5 mL of CF 3 CH 2 OH. The resulting solution was then cooled to -40 °C with oxygen bubbling through it. After several minuteε, the reaction solution was irradiated with a 1000 W sodium lamp for 24 min to produce the dioxetane as shown by the appearance of a new material by TLC which eluted immediately below the alkene and emitted light when heated. Example 4. Synthesis of Compound 4. 4- (p-Fluorophenylthio) - 4- (3-pivaloyloxyphenyl)spiro[1, 2-dioxetane-3, 2 ' tricyclo- [3.3.1.1 3 - 7 ]decane] . (a) Syntheεis of [ (3-pivaloyloxyphenyl) - (p-fluorophenyl- thio)methylene]tricyclo[3.3.l.l 3 - 7 ]decane. The pivalate- protected ketone described in example 3(a) was converted to the vinyl sulfide by stirring 0.63 g (1.85 mmol) of the ketone, 30 mL of dry THF and 0.22 mL (1.1 eq.) of TiCl 4 . The reεulting orange solution was εtirred under argon for 5 minutes. A solution of 0.57 mL (2.2 eq.) of Et 3 N and 0.22 mL (1.1 eq.) of p-fluorothiophenol in 30 mL of dry THF was then added dropwise to the reaction εolution over 30 min while the reaction εolution turned purple. Stirring was maintained for ca. 72 hours. The excesε TiCl 4 was neutralized by the addition of ca. 2 mL of ethanol, the solvents evaporated and the residue purified by chromatography using 1-10% ethyl acetate in hexane. The alkene (0.35 g) was obtained as an oil: X H NMR (CDC1 3 ) δ 1.330 (s, 9H) , 1.70-2.05 (m, 12H) , 2.775 (br ε, IH) , 3.694 (br ε, IH) , 6.78-7.20 (m, 8H) . (b) Synthesis of 4- (p-Fluorophenylthio) -4- (3-pivaloyloxy¬ phenyl)spiro[1, 2-dioxetane-3 ,2 'tricyclo[3.3.l.l 3 - 7 ]decane] (4.) . small photooxygenation apparatus was charged with 54 mg (0.12 mmol) of the vinyl sulfide, 150 mg of polymer- bound Rose Bengal, and 25 mL of CH 2 C1 2 dried over MgSθ4. The resulting solution was then cooled to -78 °C with oxygen bubbling through it. After several minutes, the reaction mixture was irradiated with a 1000 W sodium lamp for 3.5 hours. TLC using 10% ethyl acetate in hexane showed conversion to a new material which emitted light when the plate was heated. The sensitizer was filtered off, the solvent evaporated and the material purified by chromatography on silica with 20% ethyl acetate in hexane which yielded 30 mg of slightly yellow oil which emitted light when a sample was heated on a TLC plate and additionally produced red chemiluminescence when triggered with DMSO/TBAF. ---H NMR (CDCl 3 ) δ 1.375 (s, 9H) , 1.68-1.97 (m, 12H) , 2.66 (br s, IH) , 3.59 (br ε, IH) , 6.74-7.95 (m, 8H) . Example 5. Synthesis of Compound 5. 4- (2,2,2-trifluoro- ethylthio) -4- (3-hydroxyphenyl)spiro[1,2-dioxetane-3,2 ' - tricyclo[3.3.1.1 3 - 7 ] decane]. (a) Synthesiε of [ (3-hydroxyphenyl) (2,2,2-trifluoroethyl- thio)methylene] tricyclo[3.3.1.I 3 - 7 ]decane. An argon-purged round bottom flaεk was charged with 1.0 g (3.9 mmol) of the ketone of Example 2(a), 40 mL of anhydrous THF and 1.0 mL (2.3 eq.) of TiCl . The resulting brown solution was stirred under argon for 30 minuteε at room temperature. A εolution of 2.4 mL (4.4 eq.) of triethylamine dry Et3N and 0.38 mL (1.1 eq.) of 2,2,2-trifluoroethanethiol in 60 mL of dry THF waε then added dropwise to the reaction solution over 1.5 hours while the reaction solution darkened to a deep reddish brown. Stirring was maintained overnight. A portion of dry Et3N (10 mL) was added to the reaction solution followed by 10 mL of methanol. The reaction mixture was allowed to stir for an hour and then εuction filtered. The filtrate waε evaporated yielding a gummy yellow solid. This solid was then taken up in CH 2 C1 2 and the resulting mixture washed with water (emulsion formed) . The CH 2 Cl 2 layer was then dried over MgS04, filtered and concentrated in vacuo yielding a yellow solid which smelled of thiol. The solid was chromatographed on silica gel using CH 2 C1 2 as eluent. The vinyl sulfide and the starting ketone were recovered. The solvents were removed in vacuo from the first eluted fraction yielding 0.16 g of light yellow oil. i H NMR (CDC1 3 ) δl.65-2.02 (m, 12H) , 2.624 (br s, IH) , 2.791 (q, 2H, J=9.8 Hz coupled to F) , 3.609 (br s, IH) , 4.892 (s, IH) , 6.70-6.86 (m, 3H) , 7.207 (t, IH) ; 13 C NMR (CDC1 3 ) δ27.949, 33.899 (q, J=31 Hz), 35.901, 35.962, 36.842, 38.906, 39.240, 114.244, 116.611, 122.591, 125.70 (q, J=277 Hz), 129.420, 140.226, 155.190, 155.403. (b) Synthesis of 4- (2, 2,2-trifluoroethylthio) -4- (3- hydroxyphenyl)spiro[1,2-dioxetane-3,2 ' -tricyclo[3.3.l.l 3 - 7 ] - decane] (5_) . A small photooxygenation apparatus was charged with 47.2 mg of the vinyl sulfide, 2 mg of methylene blue, and 20 mL of dry CH2CI2. The resulting solution was then cooled to -78 °C with oxygen bubbling through it. After several minutes, the reaction solution was irradiated with a 1000 W sodium lamp for 45 min to produce mainly the dioxetane as shown by the light emitted on heating the TLC plate. The solution was concentrated and the product isolated by preparative TLC using 20% ethyl acetate in hexane yielding 30.4 mg of the product as a εlightly yellow oil. Mixing a εample of the dioxetane with exceεs TBAF in DMSO produced orange-yellow light. 1 H NMR (CDC1 3 ) δl.16- 2.10 (m, 12H) , 2.31 (br s, IH) , 2.948 (dq, 2H) , 3.02 (br s, IH) , 5.35 (br s, lH) , 6.878 (dd, IH) , 7.14-7.38 (m, 3H) . Table 2. Alkoxv and Arvloxv Dioxetane Compounds Dioxetane R JL 6 CH 3 H 7 CH(CH 3 ) 2 H 8 CH 2 CH=CH 2 H 9 CH 2 CH 2 CN H 10 CH 2 CH 2 OH H 11 C 6 H 5 H 12 COCH 3 H 13 CH 2 CH 3 H 14 CH(CF 3 ) 2 H 15 CH 2 CC1 3 H 16 CH 2 CHC1 2 H 17 CH 2 CF 2 CF 3 H 18 CH 2 CF 2 CF 2 CF 3 H 19 2, 6-dif luor H 20 CH 3 COC(CH 3 ) 3 21 CH 3 Si(CH 3 ) 2 t-Bu 22 CH 3 SiPh 2 t-Bu 23 CH 3 PO(OCH 2 CH 2 CN) 2 24 CH, P0 3 Na 2 Example 6. Synthesis of Compound 6. 4- (3-Hydroxyphenyl) -4- methoxyεpiro[1,2-dioxetane-3 ,2 ' -tricyclo[3.3.1.1 3 - 7 ] decane] . The vinyl εulfide of Example Kb) (0.113 g) waε irradiated for 15 min in 20 mL of methanol containing a few crystals (< 1 mg) of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. Iodine (0.096 g) and 1 mL of 30% H 2 0 2 were added and the mixture warmed to room temperature. Progress of the reaction was monitored by the disappearance of starting material by TLC (20% ethyl acetate in hexane) and the appearance of new bands which emitted blue light when the plate was heated and by observing the color of chemiluminescence from an aliquot of the reaction solution reacted with O.l M TBAF in DMSO. The yellow emission of dioxetane 1 was gradually replaced by the blue emisεion of dioxetane 6 over a period of εeveral hourε. The εolution waε concentrated to 1-2 mL and εeparated on preparative TLC with 10% ethyl acetate in hexane. The band containing dioxetane 6 was collected, desorbed and evaporated yielding 77 mg. Example 7. Alternate Synthesis of Compound 6. The vinyl sulfide of Example Kb) (0.104 g) was irradiated for 23 min in 10 mL of CH 2 C1 2 containing a few crystalε (< 1 mg) of methylene blue with continuouε oxygen bubbling at -78 °C uεing a 1000 W Na lamp. N-Chloroεuccinimide (42.8 mg) waε added. After 10 min, 28 μL of methanol (2 eq. ) was added and the solution warmed to room temperature. After an additional 30 min, TLC showed a new material which emitted blue light when the plate waε heated. The εolution was evaporated, the blue reεidue washed with ether and the ether evaporated to produce an oil. This oil was redissolved in a small amount of CH 2 C1 2 . The product was separated by preparative TLC using 20% ethyl acetate in hexane yielding 50 mg of the product as a white solid. Example 8. Synthesis of Compound 7. 4-Isopropoxy-4- (3- hydroxyphenyl)spiro[1,2-dioxetane-3,2 ' -tricyclo[3.3.l.l 3 ' 7 ] decane]. The vinyl sulfide of Example Kb) (0.131 g) was irradiated for 13 min in 20 mL of 2-propanol containing a few crystalε (< 1 mg) of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. Iodine (0.111 g) and 1 mL of 30% H 2 0 2 were added and the mixture warmed to room temperature. Progress of the reaction was monitored by the appearance of a new material by TLC (40% ethyl acetate in hexane) which emitted blue light when the plate was heated and by observing the color of chemiluminescence from an aliquot of the reaction solution reacted with O.l M TBAF in DMSO. The yellow emiεsion of dioxetane 1 was gradually replaced by the blue emiεεion of dioxetane 7 over a period of εeveral hours. The solution was concentrated to 1-2 mL and separated on preparative TLC with 10% ethyl acetate in hexane. A band containing dioxetane 7 and adamantanone was collected, desorbed and evaporated yielding 24 mg of a white solid (Dioxetane 7): X H NMR (CDC1 3 ) δl.160 (d) , 1.257 (d) , 1.22-2.14 (m) , 3.08 (br s), 3.747 (sept), 5.641 (s) , 6.892 (dd) , 7.2 (br s) , 7.25-7.33 (m) . A second component was also isolated as a pale yellow oil which exhibited an NMR spectrum consistent with the structure: X H NMR (CDCI 3 ) δl.145 (d) , 1.253 (d) , 1.20-2.14 (m) , 3.06 (br s), 3.715 (sept), 5.77 (br s), 6.8-7.5 (m) , 7.707 (d) . Example 9. Synthesis of Compound 8. 4- (3-Hydroxyphenyl) -4- (2-propenyloxy)spiro[l,2-dioxetane-3,2 ' -tricyclo[3.3.l.l 3 - 7 ] decane]. The vinyl sulfide of Example Kb) (0.100 g) was irradiated in 20 mL of CH 2 C1 2 containing a few crystalε (< 1 mg) of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. TLC indicated that the vinyl sulfide was consumed after 10 min. N-Chloroεuccinimide (0.0446 g) waε added and the mixture maintained at -78 °C for 15 minutes. Allyl alcohol (50 uL) was added and the solution warmed to room temperature. Progress of the reaction was monitored by the appearance of a new material by TLC (20% ethyl acetate in hexane) which emitted blue light when the plate was heated and by observing the color of chemiluminescence from an aliquot of the reaction solution reacted with O.l M TBAF in DMSO. The yellow emisεion of dioxetane 1 was gradually replaced by the blue emisεion of dioxetane 8. The solution was concentrated to drynesε and the residue extracted with ether. The ether was evaporated and the resulting residue chromatographed on a silica prep. TLC plate with 20% ethyl acetate in hexane. The major band contained 47 mg of dioxetane 8: 1 H NMR (CDC1 3 ) δl.09 (m, IH) , 1.26 (m, IH) , 1.471 (dq, IH) , 1.51- 1.96 (m, 9H) , 2.195 (br s, IH) , 3.127 (br s, IH) , 3.72-3.82 (m, IH) , 4.02-4.12 (m, lH) , 5.14-5.22 (m, IH) , 5.32-5.44 (m, 2H, vinylic H and phenolic OH), 5.90-6.03 (m, IH) , 6.86-6.93 (m, lH) , 7.19 (br s, 2H) , 7.26-7.34 (m, IH) . Example 10. Synthesis of Compound 9. 4- (2-Cyanoethoxy) -4- (3-hydroxyphenyl) εpiro [1,2-dioxetane-3,2 ' -tricyclo- [3.3.l.l 3 - 7 ]decane] . The vinyl εulfide of Example Kb) (0.052 g) waε irradiated in 10 mL of CH 2 C1 2 containing a few crystals (< 1 mg) of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp for 10 min. N-Chlorosuccinimide (0.0229 g) was added and the mixture maintained at -78 °C for 15 minutes. 2-Cyanoethanol (25.8 uL) in 3 mL of dry CH 2 C1 2 was added and the solution warmed to room temperature. Progress of the reaction was monitored by the appearance of a new material by TLC (20% ethyl acetate in hexane) which emitted blue light when the plate was heated and by observing the color of chemiluminescence from an aliquot of the reaction solution reacted with O.l M TBAF in DMSO. The yellow emission of dioxetane 1 was gradually replaced by the blue emission of dioxetane 9 over a period of 45 min. The solution was concentrated to dryneεs and the residue dissolved in a minimal amount of CH 2 C1 2 and purified by prep. TLC with 25% ethyl acetate in CH 2 C1 2 ; * -*H NMR (CDC1 3 ) δ 1.01-1.11 (m,lH), 1.23-1.32 (M,1H) , 1.45-1.94 (m,10H), 2.23 (br s.lH), 2.60- 2.72 (m,lH), 2.76-2.90 (m,lH), 3.06 (br s.lH), 3.49-3.60 (m,lH), 3.69-3.81 (m,lH), 5.93 (br s.lH). 6.88-6.98 (m) , 7.19 (br s,2H), 7.325 (t,lH) . Example 11. Synthesis of Compound 10. 4- (2-Hydroxyethoxy) - 4- (3-hydroxyphenyl) spiro[1, 2-dioxetane-3 , 2 ' -tricyclo- [3.3.l.l 3 - 7 ]decane] . The vinyl sulfide of Example Kb) (0.099 g) was irradiated for 11.5 min in 15 mL of CH 2 C1 2 containing a few crystals (< 1 mg) of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. TLC indicated that the vinyl sulfide was consumed. N- Chlorosuccinimide (0.044 g) was added and the mixture maintained at -78 °C for 15 minutes. Ethylene glycol (37 uL) was added and the solution warmed to room temperature. Progress of the reaction was monitored by the appearance of a new material by TLC (20% ethyl acetate in hexane) which emitted blue light when the plate was heated and by observing the color of chemiluminescence from an aliquot of the reaction εolution reacted with O.l M TBAF in DMSO. The yellow emission of dioxetane 1 was gradually replaced by the blue emission of dioxetane 10 over a period of an hour. The solution was concentrated to drynesε and the reεidue extracted with ether. The ether was evaporated and the resulting residue chromatographed on a silica prep. TLC plate with 40% ethyl acetate in hexane. The major band contained 18 mg of dioxetane 10. Example 12. Synthesis of Compound 11. 4-(3-Hydroxyphenyl) - 4-phenoxyspiro[l,2-dioxetane-3,2 ' -tricyclo[3.3. l.l 3 - 7 ] decane] . The vinyl sulfide of Example Kb) (0.106 g) was irradiated for 10 min in 20 mL of CH 2 C1 2 containing a few cryεtals (< 1 mg) of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. TLC indicated that the vinyl sulfide was consumed. N-Chlorosuccinimide (0.047 g) was added and the mixture maintained at -78 °C for 15 minutes. Phenol (83 mg) was added and the solution warmed to room temperature. Progreεε of the reaction waε monitored by the appearance of a new material by TLC (20% ethyl acetate in hexane) which emitted blue light when the plate waε heated and by obεerving the color of chemiluminescence from an aliquot of the reaction solution reacted with O.l M TBAF in DMSO. The yellow emission of dioxetane 1 was gradually replaced by the blue emission of dioxetane 11 over a period of an hour. The solution was concentrated and the reεidue chromatographed on a εilica prep. TLC plate with 20% ethyl acetate in hexane. The major band was iεolated and found to contain a mixture of phenol, ca. 60 mg of dioxetane 11 and ca. 5 mg of adamantanone. Dioxetane 11: *H NMR (CDC1 3 ) δl.09-1.19 (m) , 1.25-1.35 (m) , 1.45-1.54 (m) , 1.56-2.14 (m) , 2.26 (br ε) , 3.28 (br ε), aromatic peakε could not be resolved from residual phenol. Example 13. Synthesis of Compound 12. 4-Acetoxy-4- (3- hydroxyphenyl)spiro[1, 2-dioxetane-3 ,2 ' -tricyclo [3.3.l.l 3 ' 7 ] decane]. The vinyl sulfide of Example Kb) (50 mg) was irradiated for 25 min in 20 mL of CH 2 C1 2 containing 2 mg of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. Mercuric acetate (400 mg) in 5 mL of 1, 1, 1,3 ,3 ,3-hexafluoro-2-propanol was added. TLC indicated the formation of two new products which emitted light upon heating the plate. The solution was evaporated, the blue residue was extracted with ether, the ether εolution filtered and evaporated to a blue oil. The oil waε rediεsolved in a small amount of CH 2 C1 2 and subjected to preparative TLC using 20% ethyl acetate in hexane. A band waε isolated which contained 40 mg of dioxetane 12. A small quantity of dioxetane 14 was also obtained. Dioxetane 12: *H NMR (CDC1 3 ) δl.06 (m, IH) , 1.27 (m, IH) , 1.48 (m, IH) , 1.54-1.90 (m, 9H) , 2.193 (s, 3H) , 2.26 (br s, IH) , 3.06 (br s, IH) , 5.66 (br ε, IH) , 6.80 (m, lH) , 7.12 (br ε, 2H) , 7.248 (t, IH) . Example 14. Synthesis of Compound 13. A εmall photooxygen¬ ation apparatus was charged with 99.1 mg of the vinyl sulfide of Example 3(a), 1-2 mg of methylene blue, 10 mL of CH 2 C1 2 and 10 mL of absolute ethanol. The resulting solution was then cooled to 0 °C with oxygen bubbling through it. After several minutes, the reaction solution was irradiated with a 1000 W sodium lamp for 15 min. NCS (40.0 mg, 1.1 eq.) was added, the reaction maintained at 0 °C for 60 min and then at room temperature for 3 h. The solvents were evaporated and the residue purified by preparative TLC, eluting with CH 2 C1 2 . A colorless oil (47.6 mg) was produced. Dioxetane 13: 1 H NMR (CDC1 3 ) δ 1.05-1.90 (m, 15H) , 2.17 (br s, IH) , 3.09 (br s, IH) , 3.29 (m, IH) , 3.55 (m, IH) , 5.68 (br ε, IH) , 6.86-7.36 (m, 4H) . Example 15. Synthesis of Compound 14. 4- (Hexafluoroisopro¬ poxy) -4- (3-hydroxyphenyl)spiro[1,2-dioxetane-3 , 2 ' -tricyclo- [3.3.l.l 3 ' 7 ]decane] . The vinyl sulfide of Example 2(b) (0.102 g) was irradiated for 10 min in 20 mL of CH 2 Cl 2 containing 2 mg of methylene blue with continuous oxygen bubbling at* -78 °C using a 1000 W Na lamp. The solution was poured into a solution of 5 mL of 1,1,1,3,3,3- hexafluoro-2-propanol in 10 mL of CH 2 C1 2 . Mercuric acetate (ca. 50 mg) in 2 mL of hexafluoro-2-propanol was added gradually and the solution stirred for 30 min. TLC showed two new materials which emitted light when the plate was heated. The solution was evaporated, the blue residue washed with ether and the ether evaporated to produce an oil. This oil was redisεolved in a small amount of CH 2 C1 2 and the product isolated by preparative TLC using 30% ethyl acetate in hexane yielding 8.1 mg of the product as a colorless oil. Reaction of a sample of the dioxetane with an excess of tetrabutylammonium fluoride in DMSO produced green chemiluminescence. X H NMR (CDC1 3 ) δl.13 (m, IH) , 1.32 (m, IH) , 1.46-2.16 (m, 10H) , 2.234 (br s, IH) , 2.994 (br s, IH) , 4.646 (br s, IH) , 5.326 (br ε, IH) , 6.86-7.40 (m, 4H) ; 19 F NMR (CDC1 3 ) δ(rel. to CFC13) -72.316, -72.013 (d) . Example 16. Synthesis of Compound 15. 4-(3-Hydroxyphenyl) - 4- (2 ,2,2-trichloroethoxy) spiro[1, 2-dioxetane-3 , 2 ' -tricyclo- [3.3.l.l 3 ' 7 ]decane] . The vinyl sulfide [ (3-hydroxyphenyl) - ethylthiomethylene]tricyclo[3.3.l.l 3 ' 7 ]decane (102 mg) was irradiated for 20 min in 10 mL of CH 2 C1 2 containing 1 mg of methylene blue with continuous oxygen bubbling at -40 °C using a 1000 W Na lamp. N-Chlorosuccinimide (1 eq. ) was added. After 10 min, 3 mL of 2,2, 2-trichloroethanol was added and the solution warmed to room temperature. After an additional 30 min, TLC showed a new material which emitted blue-green light when the plate was heated. The solution was evaporated, redisεolved in a εmall amount of CH 3 OH and evaporated. The product waε εeparated by preparative TLC using 20% ethyl acetate in hexane yielding 53.5 mg of the product as a εlightly yellow oil which contained εome decomposition products: l H NMR (CDC1 3 ) δl.12 (m, IH) , 1.30 (m, IH) , 1.46-2.16 (m, 10H) , 2.227 (br s, IH) , 3.198 (br s, IH) , 3.909 (d, IH, J=10.3 Hz), 4.133 (d, IH, J=10.3 Hz), 5.75 (br ε, IH) , 6.940 (dd, IH) , 7.06-7.42 (m, 3H) . Example 17. Svntheεis of Compound 16. 4- (2,2-Dichloro- ethoxy) -4- (3-hydroxyphenyl)spiro[1, 2-dioxetane-3, 2 ' - tricyclo- [3.3.l.l 3 - 7 ]decane] . The vinyl sulfide [(3- hydroxyphenyl) ethylthiomethylene] tricyclo [3.3.l.l 3 ' 7 ] decane (111 mg) was irradiated for 16 min in 10 mL of CH 2 C1 2 containing 1 mg of methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. N- Chlorosuccinimide (50 mg, 1 eq. ) was added. After 10 min. 60.7 μL of 2,2-dichloroethanol (2 eq. ) was added and the solution warmed to room temperature. After an additional 30 min, TLC showed a new material which emitted blue-green light when the plate was heated. The solution was evaporated, redissolved in a small amount of CH 2 C1 2 and filtered. The product was separated by preparative TLC using 20% ethyl acetate in hexane yielding 45 mg of the product as a slightly yellow oil: X H NMR (CDC1 3 ) δl.05 (m, IH), 1.27 (m, IH), 1.44-2.04 (m, 10H) , 2.23 (br s, IH) , 3.098 (br s, IH) , 3.711 (dd, IH, J=ll, 7.7 Hz), 3.866 (dd, IH, J=ll , 4 . 4 Hz ) , 5 .912 (dd , IH, 3=1 .1 , 4 . 4 Hz ) , 5 . 641 (br s , IH) , 6 . 929 ( dd, IH) , 6 . 98-7 . 42 (m, 3H) . Example 18. Synthesis of Compound 17. 4- (3-Hydroxyphenyl) - 4- (2,2, 3 ,3 , 3-pentafluoropropoxy)εpiro[1, 2-dioxetane-3 ,2 ' - tricyclo[3.3.1. I 3 ' 7 ] decane] . The vinyl εulfide of Example 2(b) (0.112 g) was irradiated for 10 min in CH 2 C1 2 containing methylene blue with continuous oxygen bubbling at -78 °C using a 1000 W Na lamp. The solution waε poured into a εolution of mercuric acetate (100 mg, 0.84 eq. ) in 5 mL of 2,2,3,3,3-pentafluoro-1-propanol and εtored at -13 °C over night. TLC indicated the formation of two new products, both of which emitted light on heating. The solution was evaporated, the blue residue washed with ether and the ether evaporated to produce an oil. This oil was redissolved in a small amount of CH 2 C1 2 and the product isolated by preparative TLC using 30% ethyl acetate in hexane yielding 41.2 mg of dioxetane 17 as a colorless oil in addition to some of the acetoxy-substituted dioxetane (12); dioxetane 17: *H NMR (CDCl 3 ) δl.04 (m, IH) , 1.28 (m, IH), 1.46-2.14 (m, 10H) , 2.23 (br s, IH) , 3.017 (br s, IH) , 3 . 658 (m, IH) , 4 . 006 (m, IH) , 5 . 13 ( s , IH ) , 6 . 93 (dd, lH) , 6 . 98-7 .28 (br s , 2H) , 7 . 338 ( t , IH) . Example 19. Synthesis of Compound 18. 4- (2,2 , 3 , 3, 4, 4, 4- Heptafluorobutoxy) -4- (3-hydroxyphenyl)εpiro[1, 2-dioxetane- 3,2 ' -tricyclo[3.3.l.l 3 - 7 ] decane] . The vinyl εulfide of Example 2(b) (0.100 g) waε irradiated for 10 min in 20 mL of CH 2 C1 2 containing 2 mg of methylene blue with continuouε oxygen bubbling at -78 °C using a 1000 W Na lamp. The solution was poured into a solution of mercuric acetate (100 mg, 0.95 eq. ) in 5 mL of 2, 2, 3 , 3 , 4, 4 , 4-heptafluoro-1- butanol. After one hour, TLC indicated the formation of two new products, both of which emitted light on heating. The solution was evaporated, the blue residue washed with ether and the ether evaporated to produce an oil. This oil was redisεolved in a εmall amount of CH 2 C1 2 and the product isolated by preparative TLC using 30% ethyl acetate in hexane yielding 26.3 mg of dioxetane 18 aε a colorleεε oil in addition to some of the acetoxy-substituted dioxetane 12; dioxetane 18: ^ NMR (CDC1 3 ) δ 1.05 (m, IH) , 1.28 (m, IH) , 1.45-1.96 (m, 10H) , 2.24 (br s, lH) , 3.029 (br s, IH) , 3.71 (m, IH) , 4.04 (m, IH) , 5.332 (br s, IH) , 6.935 (dd, IH) , 6.98-7.52 (br s, 2H) , 7.338 (t, lH); 19 F NMR (CDC1 3 ) δ -127.65, -120.58, -120.55, -120.52, -120.48, -120.46, -81.40, -81.36, -81.33. Example 20. Synthesis of Compound 19. 4- (2' , 6 ' -Difluoro- phenoxy) -4- (3-hydroxyphenyl) εpiro[1, 2-dioxetane-3 ,2 ' - tricyclo[3.3.1. I 3 - 7 ]decane] . The vinyl εulfide of Example 2(b) (0.100 g) was irradiated for 10 min in 20 mL of CH 2 C1 2 containing 2 mg of methylene blue with continuous oxygen bubbling at -40 °C using a 1000 W Na lamp. NCS (44.7 mg, 1 eq. ) was added followed after 15 min by 100 mg (2.3 eq. ) of 2, 6-difluorophenol in 3 mL of CH 2 C1 2 . The solution was allowed to warm to room temperature. After an hour, TLC showed a new material which emitted blue-green light when the plate was heated. The solution was evaporated, redissolved in a small amount of CH 2 C1 2 and the product isolated by two successive preparative TLC purifications first using 5-20% ethyl acetate in hexane and then using CH 2 C1 2 on a second plate yielding 11.2 mg of the product as a slightly yellow oil; X E NMR (CDC1 3 ) δ 1.29-2.24 (m, 13H) , 3.31 (br s, IH) , 5.00 (m, IH) , 6.68-7.6 (m, 7H) . Example 21. Synthesis of Compound 20. 4-Methoxy-4- (3- pivaloyloxyphenyl)spiro[1,2-dioxetane-3,2 ' -tricyclo- [3.3.1.l 3 ' 7 ]decane] . The vinyl εulfide of Example 4(a) (0.051 g) waε irradiated in 20 mL of CH 3 OH containing ca. 1 mg of Rose Bengal with continuous oxygen bubbling at -78 °C uεing a 1000 W Na lamp. When the alkene was shown by TLC to be completely consumed, iodine (0.096 g) and 1 mL of 30% H 2 0 2 were added and the mixture warmed to room temperature. Progresε of the reaction waε monitored by the diεappearance of dioxetane 4 by TLC (20% ethyl acetate in hexane) and the appearance of new bandε which emitted blue light when the plate waε heated and by obεerving the color of chemilumineεcence from an aliquot of the reaction solution reacted with O.l M TBAF in DMSO. The yellow emisεion of dioxetane 4 waε gradually replaced by the blue emission of dioxetane 20 over a period of two hours. The solution was concentrated to 1-2 mL and separated on preparative TLC with 5 % ethyl acetate in hexane. The band containing dioxetane 20 was collected and desorbed and evaporated to yield the product as a slightly yellow oil: 1 H NMR (CDC1 3 ) δ 1.02 (m, IH) , 1.28 (m, IH) , 1.375 (s, 9H) . 1.45-1.94 (m, 10H) , 2.17 (br ε, lH) , 3.04 (br ε, lH) , 3.232 (s, 3H) , 7.07-7.49 (m, 4H) . Example 22. Synthesis of Compound 21. 4- (3-t-Butyldimethyl- silyloxyphenyl) -4-methoxyspiro[1, 2-dioxetane-3 , 2 ' -tricyclo- [3.3.1.I 3 - 7 ]decane] . Thiε example demonstrates the inter- conversion of triggerable dioxetanes by replacing one protecting group with another. A 152 mg portion (1 mmol) of t-butyldimethylsilyl chloride was added to a solution of imidazole (69 mg, 1 mmol) in 2 mL of CH 2 C1 2 causing formation of a white precipitate. The mixture was filtered and the supernatant tranεferred into a solution containing 285 mg (0.94 mmol) of dioxetane 6 in 1 mL of CH 2 C1 2 . After standing for 1 h, the solution was filtered, washed with 2 x 1 mL of water, dried and evaporated. The residue was dissolved in hexane, filtered and evaporated producing 310 mg of dioxetane 21 as an oil which produced blue chemiluminescence on heating or addition to a solution of O.l M TBAF in DMSO. -Η NMR were identical to those reported for this compound in U.S. Patent 4,857,652. Example 23. Synthesis of Compound 22. 4- (3-t-Butyldiphenyl- εilyloxyphenyl) -4-methoxyεpiro[1, 2-dioxetane-3, 2 ' -tricyclo- [3.3.l.l 3 - 7 ]decane] . Thiε example further demonεtrates the interconversion of triggerable dioxetanes. A 950 mg portion (3.45 mmol) of t-butyldiphenylsilyl chloride was added to a solution of imidazole (236 mg, 3.47 mmol) in 25 mL of CH 2 C1 2 causing formation of a white precipitate. The mixture was filtered and 0.99 g (3.3 mmol) of dioxetane 6 was added to the supernatant. After stirring over night, the solution was filtered, washed with 3 x 20 mL of water, dried and evaporated. The residue was dissolved in hexane, washed with water, dried and allowed to crystallize at 4 °C. Dioxetane 22 (767 mg) was obtained which produced blue chemiluminescence on heating or addition to a solution of 0.1 M TBAF in DMSO. *H NMR (CDC1 3 ) δ 0.75-0.82 (m, IH) , 1.10 (s, 9H) , 1.35-1.87 (m, 11H) . 2.03 (br s, IH) , 2.85 (br s, 4H) , 6.9-7.75 (m, 14H) . Example 24. Synthesis of Compound 23. 4- (Methoxy) -4- (3-bis- (cyanoethy1)phosphoryloxyphenyl)εpiro[1,2-dioxetane-3 ,2 ' - tricyclo[3.3.1.I 3 - 7 ]decane] . Examples 24 and 25 further demonstrate the interconversion of triggerable dioxetanes. A solution of anhydrous pyridine (3.0 mL, 37 mmol) in 10 mL of CH 2 C1 2 was placed under argon and cooled to 0 °C. POCl 3 (1.608 g, 10.5 mmol) was added and the solution stirred for 15 min to cool. A solution of dioxetane 6 (1.006 g, 3.3 mmol) and pyridine (3.0 mL) in 10 mL of CH 2 C1 2 was added dropwise. The ice bath waε removed and the reaction continued as the solution warmed to room temperature. TLC (3:1 ethyl acetate/hexane) showed complete conversion of dioxetane 6 in 90 min. A εolution of 2-cyanoethanol (2.2 mL, 32 mmol) and 3.0 mL of pyridine waε added and εtirring maintained over night. The solution was evaporated to dryness yielding a white solid which was purified by chromatography on silica with 30-75 % ethyl acetate in hexane yielding dioxetane 23 as a slightly yellow oil (1.24 g) : ! H NMR (CDC1 3 ) δ 0.97 (d, IH) , 1.46- 1.89 (m, 11H) , 2 . 103 (br s , IH) , 2 . 819 ( t , 4H) , 3 . 039 (br ε , IH) , 3 . 224 (s, 3H) , 4.32-4.50 (m, 4H) , 7.30-7.60 (m, 4H); 13 C NMR (CDC1 3 ) δl9.329, 19.420, 25.521, 25.642, 31.197, 31.379, 31.925, 32.593, 32.836, 34.414, 35.932, 49.773, 62.825, 62.916, 95.121, 111.057, 116.156, 120.648, 120.709, 129.724, 136.948, 149.757, 149.848; 31 P NMR (CDC1 3 ) δ 9.45 (m) rel to ext. H 3 P0 4 . Example 25. Synthesis of Co pound 24. 4-(Methoxy)-4- (3-phosphoryloxypheny1)spiro [1, 2-dioxetane-3 , 2 ' -tricyclo- [3.3.l.l 3 ' 7 ]decane] , disodium salt. To a εolution of dioxetane 23 (1.24 g, 2.54 mmol) dissolved in 50 mL of methanol was added a solution of 1.627 g (15.35 mmol) of sodium carbonate in 10 mL of Type I water (Lumigen, Southfield, MI) . After stirring for 2 days, TLC using 30% methanol in CH 2 Cl 2 showed complete removal of the cyanoethyl groups. The solid material was filtered off and washed with 50 mL of methanol. The methanol washes and reaction solution were combined and evaporated under reduced preεεure yielding a white εolid. The solid was freed of impurities by again disεolving in methanol, filtering and evaporating the methanol and cryεtallizing from methanol/acetone yielding 0.986 g of white εolid which waε identical by X H NMR to an authentic sample (see U.S. Patent 5,004,565) . Example 26. Alternate Synthesis of Vinvl Sulfide from Example Kh) .bv Ti-Mediated Coupling (a) Synthesis of ethyl 3-methoxythiobenzoate. To an ice cooled solution of m-anisoyl chloride (8.53 g) in 10 ml of CH 2 C1 2 was added 4.85 mL of pyridine. After 10 min, ethanethiol (3.73 g) was added dropwise and the resulting solution stirred over night. The solution was diluted with 100 mL of methylene chloride, washed with saturated sodium bicarbonate then washed several times with water and dried. The thioester was obtained by evaporation of the solvent; : H NMR (CDC1 3 ) δ 1.35 (t,3H), 3.06 (q,2H), 3.85 (s,3H), 7.11-7.56 (m,4H); ι 3 C NMR (CDC1 3 ) δ 14.76, 23.53, 55.46, 111.43, 119.69, 119.78, 129.58, 138.60, 159.76, 192.02. (b)Synthesis of [ (3-methoxyphenyl) (ethylthio)methylene] tricyclo[3.3.1.13 ,7]decane. A three neck flask was purged with argon and charged with 100 mL of anhydrous THF. The flask was cooled in an ice bath and titanium trichloride (14.19 g) was added with stirring. Lithium aluminum hydride (LAH) (1.75 g) was added in εmall portionε causing a brief exothermic reaction. After all of the LAH was added, the cooling bath was removed and triethylamine (12.6 mL) was added. The black mixture was refluxed for 100 min under argon and then cooled for 10 min. A εolution of adamantanone (3.46 g) and ethyl 3-methoxythiobenzoate (1.5 g) in 50 mL of dry THF waε added dropwise over 20 min. Reaction progress was monitored by TLC with 20% ethyl acetate in hexane. The crude reaction mixture was cooled to room temperature after 120 min, diluted with hexane and decanted. The residue was washed several times using a total of ca. 500 mL of hexane. The combined hexane solutions were filtered and evaporated leaving an oil. The alkene waε purified by εubjecting the oil to prep. TLC (15% ethyl acetate/hexane; 1 H NMR (CDC1 3 ) δ 1.09-1.14 (t,3H), 1.75-1.98 (m,12H) , 2.21-2.28 (q,3H) , 2.66 (s.lH), 3.60 (s,lH), 3.82 (s,3H), 6.78-7.23 (m,4H); 13 C NMR (CDC1 3 ) δ 15.21, 25.96, 28.17, 28.48, 32.45, 35.45, 35.73, 37.07, 37.25, 39.10, 39.46, 39.98, 55.19, 112.04, 115.01, 121.08, 122 . 06 , 128 . 85 , 141. 57 , 152 . 62 , 159 .36 . (c) Synthesis of [ (3-hydroxyphenyl) ethylthiomethylene] tricyclo- [3.3.l.l 3 ' 7 ]decane. A solution of the vinyl sulfide from step (b) dissolved in dry DMF is added to a solution of sodium ethanethiolate in dry DMF under an atmosphere of argon. The mixture is refluxed for 3 hours or until TLC indicates cleavage of the methyl ether. The mixture is cooled to room temperature and carefully neutralized with dilute acid. The aqueous solution is extracted with ethyl acetate, the ethyl acetate washed with water, dried and evaporated. The residual product is purified by column chromatography. Example 27. Comparison of Rates of Base-Induced Decay of Hvdroxvphenvl Alkoxv or Arvloxv Dioxetanes. The first order decay of chemiluminescence of dioxetanes 14-19 in 0.2 M 2-methyl-2-amino-1-propanol (221) buffer, pH 9.6 containing 0.88 mM Mg +2 and 1.0 mg/mL of l-trioctylphosphoniummethyl-4- tributylphoεphoniummethylbenzene dichloride waε meaεured at 37 °C in a Turner Deεignε (Sunnyvale, CA) model TD-20e luminometer. The half-liveε of decay of chemilumineεcence (t 1/2 ) of hydroxy-dioxetaneε correlate with the times required to reach the maximum light intensity (I max ) in the alkaline phosphataεe-triggered decompoεition of the correεponding phosphate-dioxetanes under the same conditionε. The half-life of decay of lumineεcence of the hydroxy dioxetane iε, therefore, uεeful for predicting the grow-in kinetics of light emission for phoεphatase triggering of phosphate dioxetanes; i.e. a fast t 1/2 for the hydroxy dioxetane indicates that the corresponding phosphate dioxetane is expected to reach I max more quickly. It is expected that, for example, the phosphate derivatives of dioxetanes 14-19 as well as others which can be produced by the process described herein will be useful in alkaline phosphatase-linked asεayε known in the art. Table 3. Kinetics of Liσht Emission from Hydroxy Dioxetanes Prepared bv the Process of the Present Invention. Dioxetane tl 2 (min) 3 °ς 14 0 .16 15 3 . 7 16 4 . 1 17 3 . 4 18 4 . 9 19 1 .76 Example 28. Comparison of Rates of Base-Induced Decay of a Hydroxyphenyl Alkylthio Dioxetane. The first order decay of chemiluminescence of dioxetane 3 in 0.2 M 221 buffer, pH 9.6 containing 0.88 mM Mg +2 and the surfactant enhancers shown below was measured at ambient temperature. Surfactant A is CTAB, B is poly(vinylbenzyltributylphosphonium chloride), C iε poly(vinylbenzyltributylphosphonium chloride) -co-poly(vinylbenzyltrioctylphosphonium chloride) , D iε l-trioctylphosphoniummethyl-4-tributylphosphonium- methylbenzene dichloride. The results indicate the surprising discovery that the sulfur-substituted dioxetane exhibits kinetics for light emission comparable to dioxetane 6 in buffer. Table 4. Kinetics nf Liσht Emission from Dioxetane 3 in the Presence of Surfactants. Surfactant, Concentration tita (min) A 0.41 g/L 11.2 B 0.5 g/L 4.2 C 0.5 g/L 2.9 D 1.0 g/L 2.0 Example 29. Fluoride Induced Chemiluminescence of Alkoxv and Aryloxy-Dioxetanes. A portion of each of the purified dioxetanes 6-23 was separately mixed with a solution of 0.1 M tetrabutylammonium fluoride (TBAF) in DMSO causing a brief flash of blue-green light which could be εeen in a darkened room by eye. Chemilumineεcence persisted for several seconds. Light emission produced in this manner could also be produced with the dioxetane deposited on a silica gel TLC plate. Example 30. Fluoride Induced Chemiluminescence from Sulfur- Substituted Dioxetanes. A portion of each of the dioxetanes 1-5 was separately mixed with a solution of 0.1 M TBAF in DMSO cauεing a brief flash of yellow to reddish light which could be seen in a darkened room by eye. Chemiluminescence persisted for several seconds.

Claims: WE CLAIM : 1. A vinyl sulfide compound of the formula: wherein R 4 iε an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, wherein R- and R 2 are selected from branched chain alkyl, cycloalkyl and aryl groups which can optionally be joined together to form a cyclic or polycyclic group, wherein R 3 iε εelected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl and fused ring polycyclic heteroaryl groups which can include additional subεtituentε and wherein X is a protecting group which can be removed by an activating agent. 2. A vinyl sulfide compound of the formula: R 6 c=«ζ —' R 3 OX wherein R 4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, wherein R 6 C is a polycyclic alkyl group and which can optionally be subεtituted, wherein R 3 is selected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl and fused ring polycyclic heteroaryl groups which can include additional subεtituentε and wherein X iε a protecting group which can be removed by an activating agent. 3. The compound of Claim 2 wherein R 4 is selected from the group consisting of alkyl containing 1 to 12 carbon atoms which may be subεtituted with at least one halogen atom and aryl containing 6 to 20 carbon atoms which may be substituted with at least one halogen atom. •rs. 4. The compound of Claim 2 wherein R 6 Cis selected from the group consisting of subεtituted and unsubstituted adamantyl groups. 5. The compound of Claim 2 wherein R 3 is a meta-phenyl group and wherein OX is selected from the group consiεting of hydroxyl, alkoxy, εubεtituted alkoxy, acyloxy having the formula OOCR 10 wherein R 10 is selected from alkyl and aryl groups containing 2 to 20 carbon atoms, trialkylsilyloxy, triarylsilyloxy, aryldialkylsilyloxy, OPO(OR 8 ) 2 wherein R 8 is an organic group, β-D-galactosyloxy and β-D-glucuronidyloxy groups. 6. The compound of Claim 2 having the formula: 7. A compound having the formula: 8. A compound having the formula: 9. A compound having the formula: 10. A compound having the formula: 11. A compound having the formula: 12. A process for producing a vinyl sulfide compound of the formula: R 2 R 3 OX comprising reacting a ketone compound of the formula: wherein R x and R 2 are organic groups which can optionally be joined together to form a cyclic or polycyclic group, with a thioester compound of the formula: .S-R 4 R 3 OX wherein R 3 is selected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl and fused ring polycyclic heteroaryl groups which can optionally be substituted and wherein X is a protecting group which can be removed by an activating agent, wherein R 4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, in an aprotic organic solvent in the presence of a metallic reducing agent, a titanium salt and, optionally, an amine base to produce the vinyl sulfide. 13. A process for producing a vinyl sulfide compound of the formula: comprising reacting a ketone compound of the formula: R 6 C=0 wherein R 6 C iε a polycyclic alkyl group which can optionally be εubεtituted with a thioester compound of the formula: .s-R 4 R 3 OX wherein R 3 is selected from aryl, biaryl, heteroaryl, fused ring polycyclic aryl and fused ring polycyclic heteroaryl groups which can optionally be substituted, wherein X is a protecting group which can be removed by an activating agent, and wherein R 4 is an organic group containing 1 to 20 carbon atoms and optionally heteroatoms, in an aprotic organic solvent in the preεence of a metallic reducing agent, a titanium εalt and, optionally, an amine baεe to produce the vinyl εulfide. 14. The process of Claim 12 or Claim 13 wherein the titanium salt iε εelected from the group consisting of titanium trichloride and titanium tetrachloride. 15. The process of Claim 12 or Claim 13 wherein the metallic reducing agent is lithium aluminum hydride and wherein an amine base is used.

Inventors: AKHAVAN-TAFTI HASHEM, ARGHAVANI ZAHRA, EICKHOLT ROBERT A, AKHAVAN-TAFTI, HASHEM, ARGHAVANI, ZAHRA, EICKHOLT, ROBERT, A.
Applicants: AKHAVAN TAFTI HASHEM, ARGHAVANI ZAHRA, EICKHOLT ROBERT A, LUMIGEN INC, AKHAVAN-TAFTI, HASHEM, ARGHAVANI, ZAHRA, EICKHOLT, ROBERT, A., LUMIGEN, INC.
Application year: 1996
Main classification: C07C69
IPC codes: C07B61, C07C323, C07D521, C07F7, C09K11