New dosage form of hydroxocobalamin and its use in cyanide poisoning

Abstract: The invention relates to the pharmaceutical field, and more particularly that of therapeutic chemistry. It relates to a new dosage form of hydroxocobalamin which serves in the treatment of cyanide poisoning. The new pharmaceutical compositions according to the invention contain hydroxocobalamin in freeze-dried form. The hydroxocobalamin is freeze-dried in an acidic medium so as to be practically instantly redissolved in a neutral saline solution. The present invention also relates to a process for producing hydroxocobalamin-based pharmaceutical compositions as well as to the first aid kits containing these compositions. Use for the treatment of cyanide poisoning.

Description: The present invention relates to the pharmaceutical field, and more particularly to that of therapeutic chemistry. The subject of the present invention is more particularly a new dosage form of hydroxocobalamin which serves in the treatment of cyanide poisoning. Agents for combating the lethal effects of cyanide poisoning are already known. Kélocyanor®, which is provided in the form of an injectable solution, is a dicobalt salt of edetic acid. The dicobalt salt of edetic acid is ionized into cobalt edetate and into cobalt ++ ; the cobalt ++ ion combines with two cyanide - ions; the dicobalt salt further ionizes slowly to produce another cobalt ++ ion which generally combines with two cyanide - ions. The cyanide-cobalt complexes are very stable and are removed in the urine. The disadvantage of this medicinal product is that cobalt is present on its own, in ionized form, which exhibits a high degree of toxicity and causes a multitude of undesirable effects. It has already been proposed to use solutions of hydroxocobalamin to combat the poisoning caused by hydrocyanic acid and to diagnose chronic hypercyanogenesis (French Patent No. 2,153,156). In this previous patent, use was made of fully ready hydroxocobalamin compositions combined with sodium thiosulphate. These compositions were intended for acute treatment by intravenous administration and for chronic treatment by intramuscular administration. The dosage which is described therein provides for the possibility of administering very high doses of hydroxocobalamin which may be as high as 30,000 µg. These anticyanide kits therefore required considerable doses of hydroxocobalamin. Furthermore, the combination with sodium thiosulphate conferred insufficient stability on the whole unit, the sodium thiosulphate being in particular a reducing and alkaline substance. The combination of hydroxocobalamin with Na 2 S 2 O 3 for the treatment of cyanide poisoning ("Chemical Abstracts", Vol 64, 1966, column 14 849, a-d) is also known. Document JP 45 002 758 B (Chemical Abstracts, vol. 72, no. 20, abstract no. 103759) describes pharmaceutical compositions containing hydroxocobalamin, pyridoxal 5-phosphate and cocarboxylase in freeze-dried form. The present invention makes it possible to overcome these stability defects by providing a new therapeutic composition consisting of hydroxocobalamin in freeze-dried form which can be redissolved at the time of use in a saline, preferably physiological saline, solution. Physiological saline solution has the advantage of being stable because it does not become oxidized and does not become reduced, of being neutral because it is not alkaline; furthermore, it allows a practically instant dissolution of the freeze-dried product. These characteristics are important because the hydroxocobalamin stability range is relatively narrow: alkaline solutions, reducing solutions and oxidizing solutions degrade hydroxocobalamin and cause it to lose its properties. The freeze-dried form has numerous advantages; it allows indefinite preservation as well as a practically instant redissolution, provided this was performed in a slightly acidic medium. Hydroxocobalamine naturally has an alkaline pH, but the stability of hydroxocobalamin is better at acidic pH. The bibliographic information as well as the monographs "hydroxocobalamin injection" of the various pharmacopoeias show that hydroxocobalamin solutions should be stabilized especially by the addition of protective substances (amino acids). In all cases, the pH is set at values of between 3.5 and 5, whereas hydroxocobalamin base solution has a pH close to 9. The present invention is therefore characterized by the fact that a stable freeze-dried hydroxocobalamin product is obtained by carrying out the freeze-drying starting with an aqueous solution which is slightly acidic, preferably between 3.5 and 5.5. The optimum pH zone ranges from 4.0 to 5.0. The acidic pH is obtained by adding to the hydroxocobalamin solution a strong inorganic acid or an organic acid, such as for example hydrochloric acid, sulphuric acid or acetic acid, in a calculated amount. It was necessary to check that the acidification of the solution of hydroxocobalamin base with hydrochloric acid did not block the active site of the molecule and that the latter conserved its OH - CN - exchanging power. Cyanidation tests were carried out and they showed that all the cyanide ions introduced were attached. For the freeze-drying needs, it may be advantageous to add to the aqueous solution a neutral inert substance such as for example arginine hydrochloride, lactose, glycine or sodium acetate. A buffering agent, such as for example a sodium salt of an organic acid, may also be added to the aqueous hydroxocobalamin solution. According to a preferred embodiment of the invention, the solution to be freeze-dried contains only hydrochloric acid, and in this manner, the freeze-drying pellet is perfectly homogeneous. Freeze-drying in an acidic medium avoids degradation and gives in the end a purer and more stable product. The concentration of hydroxocobalamin in the solution to be freeze-dried ranges from 1.0 to 5.0 g and preferably between 2.0 and 3.0 g per 100 ml. The invention also comprises a process for producing hydroxocobalamin-based pharmaceutical compositions which consists in preparing a hydroxocobalamin solution in water, in adjusting the pH to a value ranging from 3.0 to 5.0 by the controlled addition of an inorganic or organic acid and, where appropriate, of a filler and/or of a buffering agent, and then in carrying out the freeze-drying, in preparing, moreover, ampoules of saline solution with a volume of between 20 and 150 ml. The saline solution used for the redissolution is preferably a solution of sodium chloride and in particular a solution isotonic to plasma at 9 per thousand in purified water. The subject of the invention is also the first aid kit for the treatment of cyanide poisoning, consisting, on the one hand, of the vial of freeze-dried product containing between 1.0 and 5.0 g of hydroxocobalamin and an ampoule containing from 20 to 150 ml of a saline solution allowing the redissolution of hydroxocobalamin. These kits exhibit perfect stability and can therefore be stored in medicine cabinets for a long period. The subject of the invention is also the use of the hydroxocobalamin-based kits for the manufacture of a medicament for the treatment of acute or chronic cyanide poisoning, which consists in administering, via the intravenous route, the reconstituted aqueous hydroxocobalamin solution. EXAMPLE 1 UNIT COMPOSITION 1) Formulas of the hydroxocobalamin solutions with adjustment of the pH a) Hydroxocobalamin base 0.250 g Sodium acetate 0.540 g buffer pH = 4.6 Acetic acid 0.240 g Water for injection qs 10.000 ml b) Hydroxocobalamin base 0.250 g Sodium acetate 0.188 g Sodium chloride 0.041 g Acetic acid 0.041 g Water for injection qs 10.000 ml c) Hydroxocobalamin base 0.250 g HCl 0.1N qs pH = 4.0 Water for injection qs 10.000 ml d) Hydroxocobalamin base 0.250 g HCl 0.1N qs pH = 5.0 Water for injection qs 10.000 ml e) Hydroxocobalamin base 0.250 g Sodium acetate 0.166 g Sodium chloride 0.041 g Acetic acid qs pH = 4.0 Water for injection qs 10.000 ml 2) Formulas for the hydroxocobalamin solutions with protective substances f) Hydroxocobalamin base 0.250 g Arginine hydrochloride 0.010 g Water for injection qs 10.000 ml g) Hydroxocobalamin base 0.250 g Lactose 0.033 g Water for injection qs 10.000 ml h) Hydroxocobalamin base 0.250 g Glycine 0.033 g Water for injection qs 10.000 ml Solutions f), g) and h) were divided into 2 and each half was acidified. i) The preceding formula is repeated, but in 250 ml perfusion vials containing 2.5 g of hydroxocobalamin: Hydroxocobalamin base 2.5 g HCl 0.1N qs pH = 4.0 Water for injection qs 100.0 ml EXAMPLE 2 SOLUBILITY OF THE FREEZE-DRIED PRODUCT IN PHYSIOLOGICAL SALINE 1) Procedure Add to a vial of freeze-dried product (about 2.5 g) 125 ml of physiological saline. Shake the vial vigorously for exactly 30 seconds. Filter the solution immediately on a cellulose acetate membrane with a porosity of 0.45 µm and a diameter of 47 mm(SARTORIUS ref. 11106). After draining the membrane (bottom face placed on absorbent filter paper), examine the particles which may be present therein under a microscope. Also examine the walls of the vial to check that the solubility was complete. Then introduce the filter with the collected particles into a beaker containing 25.0 ml of mobile phase used for HPLC analysis. Soak the filter rapidly and stir it in order to detach or solubilize the particles which may be present. Inject the solution obtained for HPLC. 2) Tests carried out Two tests were carried out on: 1 vial of freeze-dried product + 125 ml of physiological saline stored at laboratory temperature (21°C) 1 vial of freeze-dried product + 125 ml of physiological saline stored in a refrigerator before use (+ 5°C). 3) Results Visual examination of the empty vials makes it possible to observe that no undissolved particle remains on the walls of the vial for the 2 temperature conditions tested. Microscope examination of the membrane makes it possible to visualize a few small colourless crystals and 1 or 2 pink-coloured crystals. The result is similar under the two temperature conditions. HPLC analysis of the solutions which served to soak the filters (with the possible residue collected) indicates that the quantity of hydroxocobalamin found could represent at most 0.03% of the quantity present in the vials. The result is similar for the two storage conditions tested. This percentage in fact covers the solubilized hydroxocobalamin brought by the solution which impregnates the filter and possibly nonsolubilized hydroxocobalamin. EXAMPLE 3 STABILITY OF THE SOLUTION AFTER RECONSTITUTION DURING THE DURATION OF PERFUSION 1) Operating conditions a) Preparation of the solutions • Test with exposure to ambient laboratory temperature and light Open a vial of freeze-dried product containing 2.5 g of hydroxocobalamin. Add 125 ml of a solution of sodium chloride at 9 per thousand in purified water. Shake manually and vigorously for 30 s. Using a graduated pipette, introduce 5.0 ml of the solution obtained into a 100 ml graduated flask. Bring to the required volume with the mobile phase used for the chromatography. Inject this solution immediately for HPLC (analysis at time T0). Keep the vial containing the reconstituted solution in daylight and at room temperature (20 to 22°C) on a shelf in front of a laboratory window. The laboratory is also illuminated by neon-type lamps. After storing the reconstituted solution for 2 h, 4 h, 6 h and 8 h under the above conditions, collect samples from the vial and analyse the sample by HPLC immediately as indicated for the initial analysis at time T0. • Test with exposure under a UV lamp at 366 nm Carry out the procedure as indicated in the test with the exposure at ambient laboratory temperature and light, the reconstituted solution being stored under a UV lamp at 366 nm. Collect a sample at time T0 and after storing for 1 h, 3 h and 5 h under UV at 366 nm. b) Chromatographic analyses The area of the intact hydroxocobalamin peak and the content of related substances in the solutions reconstituted at time 0 and after storage are determined by HPLC. 2) Results The detailed results of the HPLC analyses carried out on the samples collected after various storage times of the hydroxocobalamin solutions obtained after reconstitution of a vial of freeze-dried product (about 2.5 g of hydroxocobalamin base with 125 ml of NaCl solution at 9 per thousand) are presented: in Table 1: for the reconstituted solution stored under conditions of ambient laboratory temperature and light, in Table 2: for the reconstituted solution under UV lamp at 366 nm. The absolute value of the contents of related substances measured in the reconstituted solution is not completely exact. It is calculated for an exact final volume of 125 ml. It is however comparable over the entire period of storage of the same solution. To assess the stability of the hydroxocobalamin solutions, the following should be considered in particular: the variation of the hydroxocobalamin peak area measured at each storage time compared with the hydroxocobalamin peak area measured on the same solution at time T0; the variation of the contents of related substances measured during storage compared with the contents of related substances measured at time T0 on the same solution. Variation of the content of intact hydroxocobalamin found during storage: After storing the reconstituted solution for 8 hours under conditions of ambient laboratory light and temperature and after storing for 5 hours under UV lamp at 366 nm, no significant decrease is observed in the hydroxocobalamin peak area compared with the analyses carried out at time T0 on the reconstituted solutions. If there is a decrease in the hydroxocobalamin content, this decrease is low and insufficient to be detected by HPLC given the precision of the analytical method. Variation of the contents of substances related to hydroxocobalamin during storage : The increase in the contents of substances related to hydroxocobalamin detected by HPLC, after storing for 8 hours under conditions of ambient laboratory temperature and light and after storing for 5 hours under UV lamp at 366 nm remains very limited: about 1 per cent. ASSAY OF HYDROXOCOBALAMIN AND RELATED SUBSTANCES AFTER RECONSTITUTION EXPOSURE TO DAYLIGHT RT RRT control 100% T0 T 2H T 4H T 6H T 8H 9.108 0.45 57616 58786 59985 54130 58406 59531 AREAS 10.883 0.54 66134 64167 69257 63020 68001 70589 11.882 0.59 23607 37327 49821 38212 46438 44820 13.735 0.68 20859 14089 20064 10884 16572 17123 16.388 0.82 16894 20932 29809 22837 NI 25952 17.725 0.88 179243 220728 224772 243506 299941 276644 20.052 1.00 18379040 18341948 18863300 17675508 18279040 18168326 26.043 1.30 29265 49659 30579 50000 61549 45724 38.49 1.92 9212 151908 215684 205421 194838 190731 53.145 2.65 26971 27447 22015 41413 sum Ai 402830 617596 726942 715456 767760 772527 control batch TW 61.65 mg T% sec 100.8 57917 c 1.022 mg/ml H% 17.8 control 5% A= 930847 T% as such 82.8576 RRT T 0 T 2H T 4H T 6H T 8H AM (g) 2.656 2.656 2.656 2.656 2.656 % related substances 0.45 0.32 0.32 0.33 0.30 0.32 0.33 0.54 0.36 0.35 0.38 0.35 0.37 0.39 0.59 0.13 0.20 0.27 0.21 0.25 0.25 0.68 0.11 0.08 0.11 0.06 0.09 0.09 0.82 0.09 0.11 0.16 0.13 0.14 0.88 0.98 1.21 1.23 1.34 1.65 1.52 1.00 1.30 0.16 0.27 0.17 0.27 0.34 0.25 1.92 0.05 0.83 1.18 1.13 1.07 1.05 2.65 0.00 0.00 0.15 0.15 0.12 0.25 related substances (%): 2.21 3.39 3.99 3.93 4.21 4.24 % hydroxocobalamin relative to T0 100.0% 102.8% 96.4% 99.7% 99.1% NI: not integrated ASSAY OF HYDROXOCOBALAMIN AND RELATED SUBSTANCES AFTER RECONSTITUTION EXPOSURE UNDER UV LAMP AT 366 nm RT RRT control T 0 T 1H T 3H T 5H 100% 9.423 0.43 58375 61403 57524 59669 61494 AREAS 11.38 0.52 62877 71503 66314 69342 69934 12.423 0.57 29963 47572 46343 42826 34806 14.49 0.67 24104 23374 18195 12293 9748 17.425 0.80 27503 27183 39478 38154 42460 18.797 0.87 172368 220359 207299 233187 275029 21.715 1.00 18203892 18167878 17683928 18236596 18135146 28.758 1.32 25323 28171 76643 32907 72284 43.808 2.02 7027 179220 212275 207668 212788 60.277 2.78 24889 36611 52788 sum Ai 407540 658785 748960 732657 831331 control batch TW - 61.65 mg T% sec 100.8 57917 c 1.022 mg/ml H% 17.8 control S≈ 951271 T% as 82.8576 5% such RRT control T 0 T 1H T 3H T 5H 100% AM (g) 2.656 2.656 2.656 2.656 % related substances 0.43 0.31 0.33 0.31 0.32 0.33 0.52 0.34 0.38 0.36 0.37 0.38 0.57 0.16 0.26 0.25 0.23 0.19 0.67 0.13 0.13 0.10 0.07 0.05 0.80 0.15 0.15 0.21 0.20 0.23 0.87 0.93 1.18 1.11 1.25 1.48 1.00 1.32 0.14 0.15 0.41 0.18 0.39 2.02 0.04 0.96 1.14 1.12 1.14 2.78 0.00 0.00 0.13 0.20 0.28 related substances (%): 2.19 3.54 4.02 3.93 4.46 % hydroxocobalamin relative to T0 100.0% 97.3% 100.4% 99.8% 3) Conclusion The results of this study show a satisfactory stability of the freeze-dried hydroxocobalamin product reconstituted with an aqueous solution of sodium chloride at 9 per thousand in the proportions selected for administration of the product, over a period substantially covering the time necessary for the administration of a vial of freeze-dried product by slow perfusion. The test of preservation performed under UV lamp at 366 nm appears to confirm that the light factor might not be a very critical parameter for the preservation of the hydroxocobalamin solutions for the period necessary for the administration of the product by perfusion.

Claims: Pharmaceutical compositions consisting of hydroxocobalamin in freeze-dried form, characterized in that the hydroxocobalamin is freeze-dried in an acidic medium so as to be practically instantly redissolved in a neutral saline solution. Pharmaceutical compositions consisting of hydroxocobalamin in freeze-dried form according to Claim 1, characterized in that the hydroxocobalamin is redissolved in a sodium chloride solution and especially physiological saline solution. Pharmaceutical compositions consisting of hydroxocobalamin in freeze-dried form according to Claims 1 and 2, characterized in that the acidic medium obtained by dissolution of the freeze-dried product makes it possible to obtain a stable solution which can be preserved satisfactorily. Process for producing the freeze-dried hydroxocobalamin product according to Claim 1, characterized in that the hydroxocobalamin solution is prepared in an acidic medium, optionally supplemented with a filler and in that the freeze-drying is then carried out in the container intended to be used. Process for producing the freeze-dried hydroxocobalamin product according to Claim 4, characterized in that the acidic medium for dissolution has a pH ranging from 3.5 to 5.5. Process for producing the freeze-dried hydroxocobalamin product according to Claims 4 and 5, characterized in that the pH of the solution ranges from 4.0 to 5.0. Process for producing the freeze-dried hydroxocobalamin product according to Claims 4 to 6, characterized in that the acidic pH is obtained by adding to the hydroxocobalamin solution a strong inorganic acid or an organic acid, and especially hydrochloric acid, sulphuric acid or acetic acid in a calculated amount. Process for producing the freeze-dried hydroxocobalamin product according to Claims 4 to 7, characterized in that a filler chosen from arginine hydrochloride, lactose, glycine or sodium acetate is optionally added to the aqueous solution. Process for producing the freeze-dried hydroxocobalamin product according to Claims 4 to 8, characterized in that a buffering agent consisting of a sodium salt of an organic acid is optionally added to the aqueous solution. Process for producing the freeze-dried hydroxocobalamin product according to Claims 4 to 9, characterized in that the concentration of hydroxocobalamin in the solution to be freeze-dried ranges from 1 to 5 g per 100 ml. Process for producing the freeze-dried hydroxocobalamin product according to Claim 10, characterized in that the concentration of hydroxocobalamin in the solution to be freeze-dried is preferably between 2 and 3 g per 100 ml. Process for producing hydroxocobalamin-based pharmaceutical compositions which consists on the one hand in preparing a solution of hydroxocobalamin in water, adjusting the pH to a value ranging from 3 to 5 by the controlled addition of an inorganic or organic acid and, where appropriate, of a filler and/or of a buffering agent, carrying out the freeze-drying, and on the other hand in preparing ampoules of saline solution having a volume of between 20 and 150 ml. Process for producing hydroxocobalamin-based pharmaceutical compositions according to Claim 12, in which the saline solution used for redissolving is a solution of sodium chloride and in particular a solution isotonic to plasma at 9 per thousand in purified water. First aid kit for the treatment of cyanide poisoning consisting, on the one hand, of the vial of freeze-dried hydroxocobalamin product, obtained according to Claims 4 to 11, containing between 1 and 5 g of hydroxocobalamin and, on the other hand, of an ampoule containing from 20 to 150 ml of a saline solution allowing redissolution of hydroxocobalamin. Use of hydroxocobalamin-based first aid kits according to Claim 14, for the manufacture of a medicament for the treatment of acute or chronic cyanide poisoning, which consists in intravenously administering the reconstituted solution of the hydroxocobalamin composition according to Claim 1.

Inventors: BONHOMME YVES, DURAND MICHEL, POUCHOL GERARD, POULAIN MARIE-LAURE, BONHOMME, YVES, DURAND, MICHEL, POUCHOL, GERARD, POULAIN, MARIE-LAURE, Pouchol, Gérard
Applicants: MERCK PATENT GMBH
Application year: 1996
Main classification: A61K9
IPC codes: A61K31, A61P39, A61K47, A61K9, C07H23