HUMAN GLANDULAR KALLIKREIN-1 (hK2)

Abstract: A new form of human glandular kallikrein-1 (hK2) has been found by cloning a prepro-hK2 cDNA from a human prostate cancer tissue cDNA library. This form of hK2 differs in sequence from the previously deduced sequence for hK2 in that Arg<226> is substituted by Trp. Use of a baculovirus expression vector is described for obtaining hK2 proteins, including Arg<226>-hK2 as an active mature protein directly from a culture medium.

Description: Human glandular kallikrein-1 (hk2) The present invention relates to novel proteins. In par¬ ticular, it relates to a novel form of human glandular kallikrein-1 (hK2) encoded by a newly-identified hK2 gene and use of recombinant DNA techniques to obtain proteins encoded by the hK2 genes, including the mature protein encoded by the previously recognised hK2 gene, Arg 226 -hK2, as an active protein. The human glandular kallikrein gene family is composed of genes encoding three different proteins: prostate speci¬ fic antigen (PSA), glandular kallikrein-1 (hK2) and panc¬ reatic/renal kallikrein (KLK1). These genes are located on chromosome 19 and the PSA and hK2 genes are aligned at a distance of 12 kb(l). The similarity of the coding re¬ gion of the human KLK1 gene to the coding regions of the human PSA and K2 genes is 74% and 75% respectively. The coding regions of the hPSA and hK2 genes are 85% homolo- gous and the promoter regions of the same genes are 91% homologous. The KLK1 gene encodes the true kallikrein. KLK1 has a kininogenase activity and it is expressed in kidney, pancreas and salivary gland (2). By in situ hyb¬ ridization, the hPSA and hK2 genes have been shown to be expressed only in prostatic epithelial cells. Despite the similarity of the hK2 and hPSA genes, the expression le¬ vel of the hK2 gene at mRNA level is only about 10-30% of that of the hPSA gene in the prostate (3). When tested with LNCaP cells, clear up-regulation of the mRNA levels for both hK2 and hPSA was observed in the presence of androgens (4). Recently, further results have indicated that expression of hK2 and hPSA is not in fact prostate specific as pre- viously believed. By Southern blot analysis with gene- specific oligonucleotide probes after RT-PCR, expression of all three previously identified human kallikrein genes has been detected in the human endometrium (5). hPSA has also been detected in milk of lactating women by immuno¬ assay (6). It has additionally been found that 30-40% of breast tumours as well as steroid hormone stimulated nor- mal breast tissue samples contain hPSA (7). An hK2 gene has been isolated from a human genomic fetal liver DNA library and sequenced (8a). The nucleotide se¬ quence of the five coding exons of this hK2 gene was found to encode a 261 amino acid preproprotein with a signal peptide of 17 amino acids and an activation pepti¬ de of 7 amino acids like PSA. hK2 has been found to pos¬ sess the typical catalytic triad (His41-Asp96-Serl89) of serine proteases. The presence of an aspartate residue at amino acid position 183 in hK2 fits with a trypsin-like substrate activity for this protein. In PSA, there is a serine residue at the same position which accounts for PSA in contrast exhibiting chymotrypsin-like activity (8). The function of PSA is the cleavage of semenogelin clots (9), but the function of hK2 is unknown. The concentrati¬ on of PSA in serum is increased in cancer and hyperplasia of the prostate gland and PSA is widely used as a marker for the detection and monitoring of prostate cancer (10). Because of the high homology between the hPSA and hK2 genes, an hK2 protein has, however, remained to be puri¬ fied. Also, the purity of PSA is not always unambiguous. This leads to problems with hPSA assays that are based on polyclonal or monoclonal antibodies raised against hPSA. An hK2 protein has now been obtained free of hPSA conta¬ mination by cloning hK2 cDNA from a human prostate cancer tissue cDNA library in an expression vector and expres- sing the cDNA in appropriate host cells. More specifical¬ ly, an hK2 protein has been produced by providing a bacu¬ lovirus expression vector encoding a prepro-hK2 protein in insect cells (see Examples 4 and 5). While only use of a baculovirus expression vector system for production of an hK2 protein is described herein, in particular a re¬ combinant Autographa California Nuclear Polyhedrosis vi- rus (AcNPV) containing a coding sequence for prepro-hK2 under the control of the polyhedrin promoter, other ex¬ pression vector systems commonly employed for production of human proteins may be employed. If, however, an ex¬ pression vector encoding a prepro-hK2 protein is used, for example, to transform bacterial cells, e.g. B. coli cells, it will be appreciated that unlike in the case of the above baculovirus expression vector system, the pre¬ pro-sequence will not be removed by post-translational processing to give directly the corresponding mature pro- tein, e.g. active mature Arg 26 -hK2. By cloning an hK2 cDNA from a human prostate cDNA libra¬ ry, an hK2 cDNA has now been identified which has one base difference from the coding sequence for hK2 pre- viously reported. This difference at base position 792 (C to T) equates with an amino acid change at amino acid position 226 from Arg to Trp. That this observed base difference was not an artifact of the cDNA cloning and sequencing procedure but reflects the existence of a pre- viously unidentified hK2 gene was confirmed by using PCR to amplify the hK2 gene from genomic DNA of a number of human prostate and leucocyte samples (see Example 2). In one aspect, the present invention thus provides a pro- tein which is Trp 226 -hK2 having a sequence identical to Arg 226 -hK2 apart from change of Arg 226 to Trp or which is Trp 226 -hK2 having an N- and/or C-terminal extension and which retains the ability to bind Trp 226 -hK2 antibodies or hPSA antibodies, with the proviso that where said protein is a naturally-occurring protein it is substantially free of other proteins with which it is ordinarily associated. Such proteins include, in addition to Trp 226 -hK2 in subs¬ tantially pure form, for example, proTrp 226 -hK2 or prepro- Trp 226 -hK2 in substantially pure form wherein Trp 226 -hK2 is joined at the N-terminus to the pro- or prepro-sequences previously identified for Arg 226 -hK2. A further novel pro¬ tein forming an embodiment of the present invention is Trp 226 -hK2 having the N-terminal dipeptide extension Ser- Arg. It has been found that this derivative of Trp 26 -hK2, rather than Trp 226 -hK2 per se, is obtained by expression of a cDNA encoding prepro-Trp 226 -hK2 in insect cells. It is detectable using a conventional hPSA immunoassay as commercially available for use in clinical studies thus demonstrating cross-reactivity with known anti-hPSA anti¬ bodies, but is inactive (see Example 3). The present invention additionally extends to fragments of Trp 26 -hK2 proteins as hereinbefore described which retain antigenicity and the Trp 226 amino acid residue. All such fragments and any protein having the Trp 226 -hK2 se- quence are included within the term Trp 226 -hK2 protein used hereinafter. In further aspects, the present invention provides an isolated DNA or recombinant DNA encoding a Trp 226 -hK2 protein of the invention. Preferably, such a DNA may include the natural coding sequence for Trp 226 -hK2, more preferably the complete natural coding sequence for pre- pro-Trp 226 -hK2. Such a recombinant DNA may be in the form of a vector, e.g. a plasmid or viral vector. Desirably, such a vector may be an expression vector which when pre¬ sent in host cells is capable of directing production of a Trp 226 -hK2 protein of the invention in said cells or the culture medium. As hereinbefore indicated, particularly preferred, for example, is a baculovirus expression vec- tor which when present in insect cells is capable of di¬ recting production of a Trp 226 -hK2 protein of the inven¬ tion in the culture medium or in said cells. In additional aspects, the invention provides host cells containing a vector of the invention as hereinbefore described and a method of preparing a Trp 26 -hK2 protein of the invention which comprises culturing host cells of the invention containing an expression vector under con¬ ditions whereby said protein is produced in the host cells or the culture medium and isolating said protein from said cells or medium. For example, where said cells are insect cells containing a baculovirus expression vec- tor for expression in the cells of prepro-Trp 226 -hK2, as hereinbefore indicated Trp 226 -hK2 with the N-terminal di¬ peptide extension Ser-Arg may be purified from the cultu¬ re medium. The purification protocol for this purpose may, for example, comprise a combination of ion-exchange chromatography and gel filtration chromatography steps (see Example 5). The Trp 226 -hK2 derivative thus obtained may readily be subsequently converted to Trp 226 -hK2 using techniques well known to those skilled in the art of pro¬ tein engineering. If in a baculovirus expression system as described above the prepro-Trp 226 -hK2 sequence is substituted by a sequen¬ ce encoding prepro-Arg 226 -hK2 or Arg 226 -hK2 joined to an alternative N-terminal sequence capable of processing in insect cells to give secreted mature Arg 226 -hK2, active Arg 226 -hK2 may alternatively be isolated from the culture medium of host insect cells e.g. by a purification proto¬ col comprising a combination of ion-exchange chromato¬ graphy and gel filtration chromatography steps (see Exam- pie 5). Thus, there is also now additionally provided active Arg 226 -hK2 substantially free of other proteins with which it is ordinarily associated and more particu¬ larly active Arg 226 -hK2 in substantially pure form. By active Arg 226 -hK2 is meant a protein having the mature Arg 226 -hK2 sequence and capable of hydrolyzing the synthe¬ tic chromogenic polypeptide substrate H-D-Pro-Phe-Arg- pNA.2HCl, where pNA is paranitroaniline, in an assay pro¬ cedure as described in Example 5. Active Arg 226 -hK2 ini¬ tially thus obtained may be subsequently digested to an¬ tigenic fragments. Isolation of Arg 226 -hK2 or an antigenic fragment thereof may be followed by labelling of the pro¬ tein. For use, for example, in immunoassays, a protein of the present invention may be labelled with any label conven- tionally employed for labelling proteins. Thus, for exam¬ ple, a protein of the present invention may be labelled with a radiolabel, an enzyme label (e.g. alkaline phos¬ phatase), a fluorescent label (e.g. fluorescein or rhod¬ amine) a lanthanide or biotin (which may be detected by avidin or streptavidin conjugated to peroxidase). It will be appreciated that a protein of the present in¬ vention may find use as a standard reference or to test for antibody reactivity to the protein in an appropriate immunoassay or immunohistochemical assay. Such tests may include, for example, testing for cross-reactivity to an antibody raised against hPSA, Arg 226 -hK2 or Trp 226 -hK2. A protein of the present invention in unlabelled form may find use as an immunogen for the production of polyclonal or monoclonal antibodies. The protocol employed for poly¬ clonal or monoclonal antibody production may conform with any of the conventional procedures known for antibody production. For this purpose, the protein will preferably be combined with an adjuvant, e.g. Freund's complete ad¬ juvant, in an immunising composition. A suitable proce¬ dure for monoclonal antibody production to Trp 226 -hK2 or Arg 226 -hK2 may conform, for example, with the procedure described in Clinical Chemistry (1987) 33, 103-107 for obtaining monoclonal antibodies to human prostatic acid phosphatase or the procedure described in Clinical Che¬ mistry (1990) 26, 92-95 for obtaining monoclonal antibo- dies to human prostate-specific antigen. An antibody so obtained may also be labelled in conventional manner, e.g. with a radioactive label, an enzyme label, a fluorescent label, a lanthanide or biotin. By using Trp 226 -hK2 as an immunogen for monoclonal anti¬ body production and subsequently screening hybridomas using purified Trp 226 -hK2 and commercially available purified hPSA, monoclonal antibodies can be selected which are capable of binding Trp 226 -hK2, but not hPSA in human body samples and which are thus particularly valu¬ able for diagnostic purposes. It will be appreciated that Arg 226 -hK2, or a combination of Arg 226 -hK2 and Trp 226 -hK2, may alternatively be employed in such a procedure as the immunogen. Also, Arg 226 -hK2 may be used in addition to, or instead of, Trp 26 -hK2 in the screening protocol. Such an antibody screening protocol wherein both Arg 226 -hK2 and Trp 226 -hK2 are employed may be designed to select non-hPSA binding antibodies capable of distinguishing between Arg 26 -hK2 and Trp 226 -hK2. Thus, in yet another aspect, the present invention provi¬ des an antibody, either in labelled or unlabelled form, capable of binding Trp 226 -hK2 and/or Arg 226 -hK2, but not hPSA. The invention additionally provides hybridomas cap¬ able of producing such an antibody. By use of an antibody of the invention, Trp 226 -hK2 and/or Arg 226 -hK2 may be speci¬ fically detected in a sample, e.g. a body sample. Such an antibody provides a new important tool for use in diagno- sing prostatic disease. The term "antibody" as used herein will be understood to include both complete antibody molecules and antigen-bin¬ ding fragments thereof such as Fab and F(ab') 2 fragments. Humanised antibodies and fragments thereof are also in¬ cluded within the term "antibody". It will be appreciated that in patients homozygous or heterozygous for the newly-identified Trp 226 -hK2 gene, the Trp 226 -hK2 protein sequence may serve as a marker for prostatic disease, e.g. for diagnosing cancer or hyper- plasia of the prostate gland. Thus, in a still further aspect, the present invention provides a method of diag¬ nosing prostatic disease in patients homozygous or hete¬ rozygous for the Trp 226 -hK2 gene, which comprises deter¬ mining whether there is altered expression or altered concentration of a protein encoded by said gene in pros¬ tate tissue or in human body fluids. The following examples illustrate the invention with re¬ ference to Figures 1 and 2 as described below. Fig. la to ld. Silver-stained polyacrylamide gel elect¬ rophoresis and immunoblot analysis of recombinant hK2 protein. The pure recombinant Trp 26 -hK2 protein (lane 1) and the commercial hPSA (lane 2) were silver-stained in native (la) and reduced SDS-PAGE (lb). Rabbit polyclonal antibody raised against hPSA purified from seminal fluid was used to detect recombinant Arg 226 -hK2 (lane 1), recom¬ binant Trp 226 -hK2 (lane 2 and commercial hPSA (lane 3) on blotted native PAGE (lc) and reduced SDS-PAGE (Id). Fig. 2A and 2B. Quantitative recoveries of recombinant Trp 26 -hK2 and commercial hPSA by a time-resolved fluo- roimmunoassay (IA) and IRMA (IB). Pure Trp 226 -hK2 and com¬ mercial hPSA were diluted with the bovine serum albumin containing zero buffers of the kits to concentrations of 1, 10, 25, 50, 100 and 500 μl. These concentrations were assayed by the time-resolved fluoroimmunoassay and IRMA kits for hPSA. The data are means ±SD from three to five separate determinations. Example 1: Cloning of an hK2-cDNA from a human pros¬ tate cancer tissue cDNA library An hK2 cDNA was amplified from a human prostate cancer tissue cDNA library by PCR. For this PCR amplification, the N-terminal oligomer was (5'-TCCCCCGGGAGATCTCACCATG- TGGGACCTGGTTCTC-3' ) and it contained Smal and Bglll rest¬ riction sites. The C-terminal oligomer was (5'-CGCTCTA- GATCAGGGGTTGGCTGCGATGGT-3' ) and contained an Xbal rest- riction site in addition to the partial hK2 sequence. After confirmation of the hK2 sequence in the vector PCRII (Invitrogen) by the dideoxy method (Sanger et al . Proc. Natl . Acad. Sci USA (1977) 74, 5463), the prepro- hK2 cDNA was inserted into the Bglll/Xbal site of the transfer vector pVL1392 (Invitrogen). The coding sequence of this cDNA differed from the human DNA coding sequence for hK2 previously reported by Sched- lich et al. (8a) in that coding position 792 was T rather than C. Example 2: Sequence analysis of hK2 genes in human prostate tissue and leukocyte samples - detections of the Arg226Trp-polymorphism Genomic DNA was isolated from human prostate tissue obtained by prostatectomy, biopsy or transurethral resection, and from human blood leukocytes. Female and young male blood leukocyte DNAs were used as control ma- terial. Specific oiigonucleotides were used for PCR amp¬ lification and sequencing of the hK2 gene. For amplifica¬ tion, the N-terminal oligomer was 5'TTCTCACTGTGTCTCTCCTC- C-3' and the biotin-labelled C-terminal oligomer was 5'G- TGGGACAGGGGCACTCA-3' . For PCR direct sequencing, the fluorescein amidite labelled oligomer was 5'ATCATGGGGCCC- TGAGCC-3' . Variation was found at base position 792 (C or T). Se¬ quenced DNA samples from both tissue and leukocyte speci¬ mens of 36 patients with prostatic diseases revealed that there occurs a polymorphism at this base position (Table la). In this limited specimen material, 13 out of 24 prostatic cancer patients were heterozygotes CT, 10 homo¬ zygotes CC and one homozygote TT. Eight of the 12 sequen¬ ced benign prostatic hyperplasia specimens were heterozy¬ gotes CT and four homozygotes CC. The same changes were detected in the tissue and leukocyte specimens. In the control material five female blood leukocyte DNA speci¬ mens out of 10 were heterozygotes CT, four homozygotes CC and one a homozygote TT, and in addition four young male blood leukocyte DNA specimens out of 6 were heterozygotes CT and two homozygotes CC. The frequency of Arg 226 -allele was 69 % among prostatic cancer patients (N=24), 67 % among benign prostatic hyperplasia patients (N=12) and 66 % among control material (N=16), and the frequency of Trp 226 -allele was 31 %, 33 % and 34 %, respectively (Table lb). Example 3: Quantitative recovery of recombinant Trp 26 -hK2 The protein concentration of purified recombinant Trp 226 - hK2 was estimated by the method of Lowry et al . (J. Biol. Chem. 1951; 193:265) with bovine serum albumin (Bio-Rad, Richmond, CA) as the standard. The recoveries of recom¬ binant Trp 226 -hK2 were further measured by time-resolved fluoroimmunoassay (DELFIA PSA kit, Wallac, Finland) and IRMA (Tandem ®-R PSA kit, Hybritech Europe, Belgium). For the assay, the recombinant Trp 226 -hK2 and commercial PSA were diluted with the "zero" standards of immunoassay kits containing bovine serum albumin. The recoveries of Trp 226 -hK2 and commercial hPSA tested in a fluoroimmunoassay detecting hPSA were 128 ± 13 % (mean ± SD, n = 20) and 107 ± 20 % (mean ± SD, n = 20), respec¬ tively, when compared to the calibrator of the fluoroim¬ munoassay kit. The recoveries when tested in an IRMA de- tecting hPSA were 110 ± 14 % (mean ± SD, n = 15) and 98 ± 6 % (mean ± SD, n = 15), respectively, when compared to the calibrator of the IRMA kit (Fig. 2). The assays intended to measure serum hPSA concentrations were not specific to hPSA, but detected 100 % of inactive hK2 added. The active recombinant hK2 was equally detec¬ table in commercial fluoroimmunoassay and IRMA for hPSA. Example 4: Cloning of prepro-hK2 cDNA in a baculovi- rus expression vector and expression in insect cells The same cloning procedure was employed as described in Vihko et al . Proc. Natl . Acad. Sci USA (1993) 90, 799-803 starting with co-transfection of AcNPV DNA (Invitrogen) and a PVL1392 transfer vector containing a prepro-hK2 cDNA obtained as in Example 1 into Spodoptera frugiperda (Sf9) cells (Invitrogen). The culture medium of insect cells containing recombinant AcNPV DNA was assayed for secreted protein capable of binding to hPSA monoclonal antibodies using an hPSA fluoroimmunoassay kit (DELFIA, allac). Insect cells containing either a coding sequence for prepro-Trp 226 -hK2 or prepro-Arg 226 -hK2 were found to secrete into the culture medium protein capable of detection by the hPSA assay, although higher expression was observed with the prepro-Trp 226 -hK2 coding sequence. Example 5: Purification of recombinant Trp 226 -hK2 protein and active Arg 26 -hK2 The harvested medium from the recombinant virus infection was concentrated with a Pellicon cassette system (cut¬ off, 10 kDa, Millipore) and dialyzed into 50mM sodium acetate buffer (pH 5.5). The concentrate was loaded onto a cation-exchange column (S-Sepharose HP 35/100, Pharma¬ cia). After washing, the hK2 protein was eluted from the column with a linear salt gradient from 0.1 M to 0.25 M NaCl. Those fractions which were immunoreactive with po¬ lyclonal hPSA antibody (slot blot) were concentrated (Amicon) for gel filtration chromatography (Sephacryl S-75, Pharmacia) and eluted with lOmM Tris-HCl at pH 7.0 containing 150 mM NaCl. The hK2 containing fractions were pooled and dialyzed in 20 mM sodium phosphate (pH 7.0) for cation exchange chromatography (Mono-S, Pharmacia). The hK2 protein was eluted from the column with a linear NaCl gradient (0-60 mM). The S-Sepharose and the Sephac¬ ryl S-75 columns were connected to BioPilot and the Mono- S column to an FPLC automated chromatography system (Pharmacia). The purity of recombinant Trp 226 -hK2 protein thus obtained was evaluated by SDS-PAGE, native PAGE and isoelectric focusing either by silver-staining or immunostaining (12). By reduced SDS-PAGE (Fig. lb, Id), the molecular weight (Mr) of the recombinant Trp 226 -hK2 protein was found to be approximately 33 kDa (12) while the Mr of commercial hPSA was found to be 34 kDa. When analysing the recombinant Trp 26 -hK2 protein by ion spray mass spec¬ trometry (ISMS), the detected average Mr was 27.4 kDa. In the case of silver-stained native PAGE (Fig. la, lc), the recombinant Trp 226 -hK2 protein showed one band of 370 kDa while commercial hPSA showed four bands between 70 and 140 kDa. The heterogeneity seen with hPSA was possibly due to endoproteolytic cleavage of the protein into 2 or 4 polypeptide chains held together by disulphide bridges (13). N-terminal amino acid analysis showed that posttransia¬ tional processing did not completely remove the prepro- sequence of prepro-Trp 226 -hK2 in the insect cells. Cleavage occurred between Gln-3 and Ser-2 rather than between Arg-1 and Ile-1. This form of recombinant hK2 was found to be inactive when assayed with synthetic polypep¬ tides using the following assay procedure: hK2 hydrolysis assay Hydrolysis of H-D-Pro-Phe-Arg-pNA.2HCl and MeO-Suc-Arg- Pro-Tyr-pNA.HCl (Chromogenix AB) at a final concentration of 1 mM was measured at 405 nm. The reactions were per- formed at 370°C and initiated by addition of the chromo¬ genic substrate (50 ml) to 200 ml of 50 mM Tris buffer (pH 7.8) with 100 mM NaCl containing hK2 protein. After one hour, the reaction was stopped by adding 800 ml of 0.6 M acetic acid and the reaction rate (nmol pNA formed per ml) was calculated from a standard curve of pNA. In serum, PSA predominately exists as a complex with αl- antichymotrypsin (15). Serine protease inhibitors like αl-antichymotrypsin and αl-antitrypsin usually react with the active site of the proteinase (16). It has been found, however, that recombinant Trp 226 -hK2 protein ob- tained as above does not complex with either of these serpins. The purification procedure given above may also be applied to recover Arg 226 -hK2 from a culture medium. By this means, insect cells containing a prepro-Arg 226 -hK2 coding sequence in an AcNPV vector operably-linked to a polyhedrin promoter have been shown to produce an active hK2 protein with trypsin-like activity. The immunostained native PAGE of the recovered Arg 226 -hK2 protein showed a different pattern when compared to the recombinant Trp 226 - hK2 protein (Fig. lb and lc). As hereinbefore indicated, purified recombinant hK2 pro¬ teins obtained by the above procedure and purified mature Trp 226 -hK2, e.g. obtained by further processing of a Trp 226 - hK2 derivative of the type discussed above, can be used as antigens for monoclonal antibody production. By using, for example, mature Trp 226 -hK2 or mature Arg 225 -hK2 for mo¬ noclonal antibody production in accordance with the pro- cedure described by Hδyhtya et al in Clin. Chem. (1987) 33, 103-107 and screening hybridomas with one or both of Arg 226 -hK2 and Trp 226 -hK2 in addition to commercially avail¬ able purified hPSA a monoclonal antibody can be obtained capable of distinguishing hK2 from hPSA in samples inclu- ding human body samples, e.g. samples derived from human prostatic tissue. Such an antibody may be desirably la¬ belled with Eu atoms as described in Vihko et al. Clini¬ cal Chemistry (1990) 26, 92-95. REFERENCES 1. B.A. Evans et al . , Biochem. 27, 3124 (1988). P.H.J. Riegman et al., FEBS Lett. 247, 123 (1989). P.H.J. Rieg- man et al., Genomics 14, 6 (1992). 2. A.R. Baker and J. Shine, DNA 4, 445 (1985). D. Fukushima et al . , Biochemistry 24, 8037 (1985). 3. P. Chapdelaine et al., FEBS Lett 236, 205 (1988). S.-D. Qiu et al., J. Urol . 144, 1550 (1990). C.Y.-F. Young et al . , Biochem 31, 818 (1992) L. Hakalahti et al., Int. J. Cancer 55, 1 (1993). 4. P. Henttu et al . , Endocrinology 130, 766-772 (1992). 5. J. Clements and A. Mukhtar, Journal of Clinical Endocrinology and Metabolism 78, 1536 (1994). 6. H. Yu and E.P. Diamandis, Clin. Chem 41, 54 (1995). 7. H. Yu et al., Clin. Biochem. 27, 75 (1994). 8. (a) L. Schedlich et al . , DNA 6, 429 (1987). (b) H. Neurath et al.. Science 158, 1638, (1967) (c) J. Schaller et al., Eur. J. Biochem. 170, 111 (1987) (d) K.W.K. Watt et al., Proc. Natl . Acad. USA 83, 3166 (1986). 9. H. Lilja, J. Clin. Invest. 76, 1899 (1985). 10. T.A. Stamey et al., New Engl . J. Med. 317, 909 (1987). 11. P. Vihko et al., Proc. Natl. Acad. Sci USA 90, 799, (199R) 12. T.M. Jovin, Biochem. 12, 871 (1973). M. Wyckoff et al., Λnal. Biochem, 78, 459 (1977). B.J. Davis, Ann. N. Y. Acad. Sci 121. 404 (1964). A.T. Andrews, in Electrophoresiε , Theory, Techniques and Biochemical and Clinical Applications (A. R. Peacoce and W. F. 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Claims: Claims 1. A protein which is Trp 226 - human glandular kalli¬ krein-1 (Trp 226 -hK2) having a sequence identical to Arg 226 - hK2 apart from change of Arg 226 to Trp, or which is Trp 226 - hK2 having an N- and/or C- terminal extension and which retains the ability to bind Trp 226 -hK2 antibodies or human prostate specific antigen antibodies, with the proviso that where said protein is a naturally-occurring protein it is substantially free of other proteins with which it is ordinarily associated. 2. A protein according to claim 1 selected from pro- Trp 226 -hK2 or prepro-Trp 226 -hK2 wherein Trp 226 -hK2 is joined at the N-terminus to the pro- or prepro-sequences of pre- pro-Arg 226 -hK2. 3. The protein according to claim 1 which is Trp 226 -hK2 having the N-terminal dipeptide extension Ser-Arg. 4. Trp zz -hK2 in substantially pure form. 5. An antigenic fragment of a protein according to any one of claims 1 to 4 which retains the Trp 226 amino acid residue of Trp 226 -hK2, with the proviso that where said protein is a naturally-occurring protein it is substan¬ tially free of other proteins with which it is ordinarily associated. 6. An isolated DNA encoding a protein as claimed in any one of claims 1 to 5. 7. A recombinant DNA encoding a protein as claimed in any one of claims 1 to 5. 8. A recombinant DNA as claimed in claim 7 which is in the form of a vector. 9. A vector as claimed in claim 8 which is an expressi¬ on vector wherein a coding sequence for a protein as claimed in any one of claims 1 to 5 is operably-linked to a promoter sequence capable of directing expression of said coding sequence in insect cells. 10. An expression vector as claimed in claim 9 which is a baculovirus expression vector wherein a coding sequence for a protein as claimed in any one of claims 1 to 5 is operably-linked to a promoter sequence capable of direc¬ ting expression of said coding sequence in insect cells. 11. A baculovirus expression vector as claimed in claim 10 which contains a coding sequence for prepro-Trp 226 -hK2 under the control of a promoter capable of directing ex¬ pression of prepro-Trp 226 -hK2 in insect cells. 12. A baculovirus expression vector as claimed in claim 11 which is a recombinant AcNPV vector wherein the coding sequence for prepro-Trp 226 -hK2 is under the control of the polyhedrin promoter. 13. A host cell containing a vector as claimed in any one of claims 8 to 12. 14. A method of preparing a protein as claimed in any one of claims 1 to 5 which comprises culturing host cells containing an expression vector as claimed in any one of claims 9 to 12 under conditions whereby said protein is produced in the host cells or the culture medium and isolating said protein from said cells or medium. 15. A method as claimed in claim 14 wherein insect cells containing a baculovirus expression vector as claimed in claim 11 or claim 12 are employed and Trp 226 -hK2 with the N-terminal dipeptide extension Ser-Arg is isolated from the culture medium. 16. A method as claimed in claim 15 wherein said isola¬ tion comprises ion-exchange chromatography and gel filt¬ ration chromatography. 17. A method for preparing Trp 226 -hK2 which comprises carrying out a method as claimed in claim 15 or claim 16 and converting said Trp 226 -hK2 derivative to Trp 226 -hK2. 18. Use of a protein as claimed in any one of claims 1 to 5 as an immunogen to obtain antibodies capable of binding said protein. 19. Use of a protein as claimed in any one of claims 1 to 5 as an immunogen to obtain a monoclonal antibody ca- pable of binding said protein. 20. Use of a protein as claimed in claim 18 or claim 19 which further comprises labelling said antibodies or antibody. 21. A protein as claimed in any one of claims 1 to 5 which is labelled. 22. Use of a protein as claimed in any one of claims 1 to 5 or 21 as a standard reference or to test for antibo¬ dy reactivity to said protein in an immunoassay or im¬ munohistochemical assay. 23. A method of diagnosing prostatic disease in patients homozygous or heterozygous for the Trp 226 -hK2 gene, which comprises determining whether there is altered expression or altered concentration of a protein encoded by said gene in prostate tissue or in the human body fluids. 24. A baculovirus expression vector as claimed in claim 11 or claim 12 in which the prepro-Trp 226 -hK2 coding se¬ quence is substituted by a sequence encoding prepro- Arg 226 -hK2 or Arg 226 -hK2 joined to an alternative N-terminal sequence capable of processing in insect cells to give secreted active Arg 226 -hK2. 25. An insect cell containing a baculovirus expression vector as claimed in claim 24. 26. A method of preparing active Arg 226 -hK2 which comp¬ rises culturing insect cells as claimed in claim 25 under conditions whereby active Arg 226 -hK2 is produced in the culture medium and isolating active Arg 226 -hK2 from said medium. 27. A method as claimed in claim 25 wherein said isola- tion comprises ion-exchange chromatography and gel filt¬ ration. 28. A method as claimed in claim 26 or claim 27 which further comprises forming and isolating an antigenic fragment of Arg 226 -hK2. 29. A method as claimed in any one of claims 26 to 28 which further comprises labelling of Arg 226 -hK2 or an antigenic fragment thereof. 30. Active Arg 226 -hK2 obtainable by a method as claimed in claim 26 substantially free of other proteins with which it is ordinarily associated. 31. Active Arg 2 -hK2 in substantially pure form. 32. Arg 226 -hK2 as claimed in claim 30 or claim 31 which is labelled. 33. Use of Arg 226 -hK2 as claimed in claim 30 or claim 31 as an immunogen to obtain antibodies capable of binding said protein. 34. Use of Arg 226 -hK2 as claimed in claim 30 or claim 31 as an immunogen to obtain a monoclonal antibody capable of binding said protein. 35. Use of Arg 26 -hK2 as claimed in claim 33 or claim 34 which further comprises labelling said antibodies or antibody. 36. Use of Arg 226 -hK2 as claimed in any one of claims 30 to 32 as a standard reference or to test for antibody reactivity to said protein in an immunoassay or immuno¬ histochemical assay. 37. An antibody capable of binding Trp 226 -hK2, but not hPSA. 38. An antibody as claimed in claim 37 which is label¬ led. 39. Use of an antibody as claimed in claim 37 or claim 38 to detect Trp 226 -hK2 in a sample. 40. A hybridoma capable of producing a monoclonal anti¬ body according to claim 37. 41. An antibody capable of binding Arg 226 -hK2 and/or Trp 226 -hK2, but not hPSA. 42. An antibody as claimed in claim 41 which is label- led. 43. Use of an antibody as claimed in claim 41 or claim 42 to detect Arg 226 -hK2 and/or Trp 226 -hK2 in a sample. 44. A hybridoma capable of producing a monoclonal anti¬ body according to claim 41.

Inventors: VIHKO PIRKKO, VIHKO, PIRKKO
Applicants: ORION YHTYMAE OY, VIHKO PIRKKO, ORION-YHTYMAE OY, VIHKO, PIRKKO
Application year: 1996
Main classification: C12N9
IPC codes: A61K38, C07K16, C12N5, C12N9, C12N15, C12P21, G01N33