METHOD AND KIT FOR PREDICTING THE THERAPEUTIC RESPONSE OF A DRUG AGAINST A MALIGNANT TUMOUR

Abstract: The present invention relates to a method of predicting the therapeutic response of a drug against a malignant tumour in a human patient, using a sample of a fine needle aspirate of the tumour from the patient. The method comprises the steps of identifying and estimating in the sample the number of cells belonging to one class of cells in relation to other cells in the sample, and comparing the estimation with a previously made analysis demonstrating correlation between the number of the cells and parameters of the therapeutic response of the drug directed towards the tumour in human patients, the results of the comparison being indicative of the therapeutic response of the drug in human patient. A diagnostic kit comprises a mixture of enzymes rendering the sample of the tumour a single cell suspension, a first mixture of monoclonal antibodies directed towards tumour associated antigens on tumour cells in the single cell suspension, said antibodies having a first label, and a second mixture of two types of monoclonal antibodies directed towards two specific antigens on lymphocytes, said antibodies having a second and a third label, respectively.

Description: METHOD AND KIT FOR PREDICTING THE THERAPEUTIC RESPONSE OF A DRUG AGAINST A MALIGNANT TUMOUR. The present invention relates generally to a method of predicting the therapeutic response of a drug. More spe¬ cifically, the invention relates to a method of predicting the therapeutic response of a drug directed towards a malignant tumour in a human patient, and to a diagnostic kit in order to enable the method to be applied routinely. The importance of lymphocytes in the control by the immune system of malignant tumours as well as the necessary presence of these cells in tumours has been known for several decades. However, nobody has studied the presence of lymphocytes in tumours, using fine needle aspirates, in relation to immunotherapy and the effect of treating a disease with a successful outcome. The outcome of a treatment of a patient with a speci- fie drug against a malignant tumour is often unpredictable. Only about 15-20 % of the patients respond to the medica¬ tion of each type of treatment. The patients receiving the medication are subjected to an unnecessary suffering since adverse reactions often are obtained from the drug used. Mostly, the effect of the drug is not shown before 3-6 months of treatment. A drug for treatment of a malignant tumour is often expensive and a considerable cost reduction would be obtained if the drug used is effective. It would therefore be of great importance if patients with a high probability to respond could be identified before the onset of treatment . For example, interferon-alpha (IFN-α) has a documen¬ ted activity against malignant melanoma. Five to ten mil¬ lion units IFN-α three times weekly seems, at the cost of reasonable side effects, (Legha et al . ) to be an optimal dose range. The treatment efficacy varies in different stu- dies (Creagan et al. , Gundersen, Legha et al. ) and the over-all response rate (CR+PR) to IFN-α alone is only about 20 % (Creagan et al. , Legha et al. ) . It would therefore be a considerable improvement if patients with a high probabi- lity to respond to this treatment could be identified using predictive tests. Besides having an antiproliferative activity (Balkwill et al, .Riviere et al. ) , the antitumour effect of IFN-α can be due to modulation of tumour cells, e.g. indue- tion of 2,5 -A synthetase or increased expression of cell surface proteins, MHC I and tumour associated antigens which are of importance for the immunological control of tumours. In addition, IFN-α modulates several immune functions (12 Friedman) . There are still no firm data de- monstrating which of these activities of IFN-α that is the most important one for the antitumour activity or if they all contribute. As IFN-α modulates the activity of various cells in the immune system, e.g. activation of machrophages, NK- cells, cytotoxic T-cells and B-cell function (Friedman et al. ) , the therapeutic effect might depend on the immune status of the patients when IFN-α therapy is initiated. Immune reactivity to malignant melanoma has been de¬ monstrated as spontaneous regression (Mc Govern et al . , Sondergaard et al. , Ronan et al. , Kang et al . ) , occurence of antibodies of prognostic significans against melanoma associated antigens and specific cytotoxic lymphocytes (Abershold et al.) . Conflicting results have, however, been reported on the prognostic significance of lymphocytic in- filtration in primary malignant melanoma. Some studies found a significantly better prognosis if the primary le¬ sions had a prominent infiltration of lymphocytes (Hansen et al. , Larsen et al. ) whereas others found no such corre¬ lation (Balch et al . ) Three studies also found a relation between tumour thickness and lymphocytic infiltration (Balch et al . , Hansen et al. , Larsen et al. ) . This discre¬ pancy was further studied by Mc Govern et al . who found the lymphocytic infiltration at the base of the melanoma to be of prognostic significance in contrast to lymphocytic in- 5 filtration at the margins. These authors also found that the lymphocytic infiltration at the tumour base was reduced as tumour thickness increased. A reasonable strategy to treat malignant melanoma would therefore, based on the im¬ mune reactivity to this type of malignancy, be to enhance 10 the anti-tumour immune reactivity by immunomodulating sub¬ stances, such as interferons and Interleukin-2. The purpose of the present invention is to produce a method of predicting the therapeutic response of a drug against a malignant tumour in a human patient, using a 15 sample of a fine needle aspirate of said tumour from said patient. The method according to the invention is thus based on a direct diagnosis in the tumour of the accumu¬ lation of lymphocytes. In order to achieve this purpose, the method accor- 0 ding to the invention has been given the characterizing feature of claim 1. The method of predicting the therapeutic response of a drug concerns patients with a regional as well as a generalised metastasis formation. It is also possible by 25 means of the method according to the invention to predict and monitor immunotherapy. MATERIAL AND METHODS Patients data. This report includes 41 patients with metastatic 30. malignant melanoma, 23 males and 18 females. Median age was 60 years (range 33 to 77) and Karnowsky performance status was 70 or more. All patients had histologically proven primary malignant melanoma and recurrences were cytolo- gically proven before start of treatment. Twenty patients 35 had regional metastases ( 20 lymphnode metastases) , which in general were excised after 1 to 3 weeks of IFN-α treat¬ ment and 21 patients had systemic disease with the fol¬ lowing metastatic sites: 2 cutaneous, 5 subcutaneous, 16 lymphnodes, 7 pulmonary, 3 bone, and 16 visceral (9 liver, 1 ovarian, 1 pancreatic, 1 vulvar, 1 adrenal gland and 3 spleen metastases) . The number of metastatic sites were 1 in 7 patients, 2 in 5 patients, 3 in 4 patients, 4 in 1 pa¬ tient and 5 or more in 4 patients. No patient had pre¬ viously been treated except for surgical removal of primary lesions or metastases. Patients with symptoms of brain me¬ tastases were not included in this study. Patients evaluation. Pre-treatment investigations included ECG, abdominal CT/US, chest X-ray, bone scintigraphy and blood samples for measurements of creatinine, bilirubin, alkaline phospha- tase, alanine-aminotransferase, lactat-dehydrogenase, alpha amylase, haemoglobin, white blood cells and thrombocytes. Treatment schedule, In a pilot study including 12 patients (4 with regio- nal disease and 8 with general metastases) , IFN-α was given s.c. three days weekly at a dose of 10 IU. In this pilot study it was decided to give Cyclophosfamide and Indometa- cin in addition to IFN-α in an attempt to enhance the the¬ rapeutic efficacy of IFN-α. Patients were treated with cyclophosphamide at a dose of 300mg/m 2 administered as i.v. bolus every three weeks and 6 of these patients were also treated with 50 mg of Indomethacin three times daily and 1 of the 6 was in addition given 400mg of Cimetidin twice daily. Tumour response was evaluated in patients with regi- onal resectable disease after one to three weeks and in pa¬ tients with distant metastases after one to three treatment cycles, one cycle being three weeks. The pilot study was followed by a confirmatory study including 29 patients (16 with regional resectable disease and 13 with systemic disease) patients were treated with IFN-α alone, sc. at a dose of 10 milj . IU three times weekly in order to demonstrate the predictive value of CD4+ tumour infiltrating lymphocytes for the outcome of IFN-α treatment. In patients with regional resectable disease the treatment was given during one to three weeks before sur¬ gery and in the group with distant metastases treatment was given 3 weeks followed by one week without treatment. No other treatments were added in either group in the confir¬ matory study. Tumour respone was evaluated in patients with regional resectable metastases after the first cycle and in patients with distant metastases after one to three treat¬ ment cycles. As no differences were found between the two studies regarding response rates or histopathological regression of tumour infiltrating inflammatory cells the results of them were added for the final analyses . Monoclonal antibodies. CD4 (Leu-3a, Becton-Dickinson) : The antigen is pre¬ sent on the helper T subset and in low density on monocytes and in the cytoplasm of monocytes and macrophages . CD8 (Leu-2a. Becton-Dickinson) : The antigen is pre¬ sent on cytotoxic/suppressor lymphocytes. The antigen is also expressed on some Leu-11+ (CD16) cytotoxic NK cells, on a subpopulation of Leu-7+ (HNK-1) cells (which do not have cytotoxic and NK activity) , on some Leu-8+ cells (which participate in suppression of B-cell function) , and on Leu-15+ (CDllb) cells which are associated with suppres¬ sor function. CDllc : (M5, Becton-Dickinson) : The antigen is present ■ on monocytes and in low density on granulocytes and large granular lymphocytes in peripheral blood. It is also ex¬ pressed on macrophages in normal lymphoid tissue, on Kuppfer cells in liver, and alveolar macrophages in lung tissue. Fine needle aspiration of metastases. Usually seven to ten aspirations were taken from each tumour with a 0.1 mm hypodermic needle. No anesthesia was needed. The aspirate was smeared on a glass slide and was allowed to dry in air. At least two smears were then stai¬ ned for conventional cytomorphology according to the May- Grunewald-Giemsa method. In cases without obvious melanin pigment in tumour cells the diagnosis of melanoma was con¬ firmed with immunostaining for vimentin and protein S-100. Morphological signs of degeneration or necrosis were regis¬ tered. Immunoloσical staining of fine needle aspirates. The slides were air dried and then fixed for 5 minu¬ tes in aceton. After drying the slides were washed in phosphate buffered saline (PBS) , pH 7.6, and incubated with monoclonal antibodies against CD4, CD8 and CDllc (see above) for 30 minutes. Mouse IgG (Sigma, Stockholm, Sweden) was used as negative control. After washing in PBS, the sections were incubated with rabbit anti-mouse immu- noglobulin (Dakopatts Z 259) and incubated for 30 minutes, washed in TBS and incubated with the PAP mouse monoclonal antibody (Dakopatts, P 850) for 30 minutes. After washing in TBS, the slides were treated with Diaminobenzidin, DAB (Sigma, Stockholm, Sweden) in 3% H2O2 for 6 minutes and washed- The slides were counterstained in Mayers haematoxy- lin for 15 minutes, washed and mounted in Glycergel (Dakopatts, Sweden) . All incubations were performed in a moist chamber. Preparation of tumour biopsies and immunoloσical ■ staining of tissue sections. Biopsies from resectable tumours were immediately snap frozen and stored at -70 °C until further processed. Frozen tissue sections, 6-7 μ thick, were fixed in acetone for 10 minutes and then air dried. They were washed in Tris-buffered saline (TBS) , pH 7.6 for 5 min, incubated with primary antibodies CD4, CD8 and CDllc (see above) for 30 minutes and then washed in TBS for 5 minutes. Mouse IgG (Sigma, Stockholm,Sweden) was used as negative control. The sections were then incubated with rabbit anti-mouse immu- noglobulin (Dakopatts, Z 259) for 30 minutes, washed in TBS and incubated with the APAAP mouse monoclonal antibody (Dakopatts D 651) for 30 minutes. After washing in TBS and incubating with the alkaline phosphatase substrate (Naphtol AS-MX Phosphate 2mg (Sigma N4875) , Dimethylformamide 0.2 ml, 0.1 M Tris buffer pH 8.2 9.8 ml, 1 M Levamisole 50μl (Sigma L-9756) and Fast-Red TR salt lOmg (Sigma F 1500) for 20 minutes, the sections were washed again in TBS. They were then counterstained in Mayers haematoxylin for 15 mi¬ nutes and mounted in Glycergel ( Dakopatts, Sweden) . All incubations were performed in a moist chamber. Evaluation of mononuclear cells. Because of the often heterogenous distribution of in¬ filtrating inflammatory cells, counting of cells per micro¬ scopic field was not performed. The overall occurence of each subset of these cells, in relation to the number of tumour cells, was instead scored as - (absent) , + (sparse, low numbers) , ++ (moderate) , +++ (high) independently by two inverstigators . CD4+ cells scored as lymphocytes had small nuclei and sparse cytoplasm with distinct cell mem- brane. In contrast, CD4+ macrophages displayed large nuclei and abundant, generally faintly staining cytoplasm. Criteria of tumour regression. Based on the description of regression in primary me¬ lanoma in other studies (Mc Govern et al . , Kang et al . , Sondergaard et al . , Ronan et al . ) the following critera of tumour regression were used in this study : 1. Low and va¬ riable density of tumour cells, particularly variation in density within the same tumour nodule, 2. Disorganisation of the architecture of the tumour with nests of remaining tumour cells surrounded by stromal tissue. The occurence of regressive areas varies from no signs of regression to al¬ most complete destruction of the tumour with only few re¬ maining tumour cells. The occurence of regressive changes of the tumours used in this study of patients with resected metastatses were estimated to be less than 25% (slight regression) , 25- 75% and more than 75 % (marked regression) . Statistical methods. The difference in distribution of inflammatory cells between patients with tumour response and progressive di¬ sease was analysed using the chi-square test. EXAMPLE 1. Treatment efficacy. Two groups of patients were studied, those with re- sectable, regional metastases and those with general meta¬ stases. In the latter group the effect of IFN-α treatment was determined as reduction in tumour size. As the main ob¬ jective of this study was to analyse the importance of tumour infiltrating inflammatory cells for the anti-tumour effect of IFN-α, significant tumour regression (more than 25%) in patients with mixed responses and regressions not fullfilling the criteria for partial remission were regis¬ tered and used in the following analyses. Among patients with general metastases 11 out of 21 patients had reduction in tumour size. One had a complete remission, 4 partial re¬ mission, 3 had a reduction of measurable tumours between 25-50 %, 3 had a mixed response, and 10 patients had pro¬ gressive disease. Patients with regional, resectable metastases were • treated with IFN-α for only one to three weeks due to the importance of not delaying surgery. This is of course too short a time to allow an adequate determination of the treatment efficacy based on measurement of the tumour size only, thus the determination of therapeutic effect in this group was based on the occurence of tumour regression in the histopathological examination of the tumour biopsies. Metastases with ongoing regression were generally permeated by CD4+ and CD8+ lymphocytes as well as CDllc+ machro- phages. 5 Histopathological criteria for signs of spontaneous regression of malignant melanoma has been described in pri¬ mary tumours. Based on such criteria (described above) nine patients with regional metastases had marked histopatholo¬ gical regression of the resected metastases and 11 patients 10 had only slight regressive changes. As no differences regarding tumour regression - de¬ termined as reduction in size or occurence of histopatholo¬ gical regressive changes - were found between the pilot study and the confirmatory study the patients of these stu- 15 dies were combined in the analyses below. The results ob¬ tained in the few (five) patients treated with IFN-α for one week did not seem to differ from the results after 2-3 weeks of treatment, thus, all patients were combined in the following analyses. 20 Subsets of tumour infiltrating mononuclear cells (MNC) . The fine needle aspirates were obtained from lymphnode, subcutaneous and liver metastases .The occurence of tumour infiltrating mononuclear cells in these aspirates 25 were studied before initiation of IFN-α treatment. The num¬ ber and distribution of various subsets of of mononuclar cells in the aspirates from the different metastases showed considerable individual variation (Table 1) . 30. Table 1. Occurence of tumour infiltrating CD4+, CD8+ and CDllc+ inflamatory cells in fine needle aspirates from pa¬ tients with regional and systemic disease before initiation of IFN-α treatment. 35 Number of CD4 CD8 CDllc infiltrating cells Low Moderate High Low Moderate High Low Moderate High Regional di¬ sease 6 4 10 9 7 3 6 8 5 Systemic di¬ sease 10 7 4 14 4 2 6 4 5 Before treatment high numbers of CD4+ cells were found in metastases from 10 out of 20 patients with regio¬ nal metastases. In all patients with high numbers of CD4+ cells more than 50 % of these cells showed morphologic cha¬ racteristics of lymphocytes. In contrast, in tumours with low numbers of these cells, CD4+ macrophages were more abundant than CD4+ lymphocytes in 2 out of 6 patients. In patients with moderate numbers of CD4+ cells macrophages were more abundant than lymphocyes in 2 out of 4 patients. In patients with general metastases high numbers of CD4+ cells were found in 4 out of 21 patients before treat¬ ment, a result which is significantly different from the frequency found in patients with regional disease (P<0.05) . In all of these patients more than 50 % of the CD4+ cells showed morphologic characteristics of lymphocytes. In aspi¬ rates from patients with low numbers of CD4+ cells macrophages were more abundant than lymphocytes in 1 out of 10 patients. In patients with moderate numbers of CD4+ cells macrophages were shown to be more abundant than lym¬ phocytes in 1 out of 7 patients. In patients with regional disease CD4+ cells predomi¬ nated over CD8+ cells in 7 out of 19 patients. In 10 pati¬ ents there were no predominance of either subsets and in 2 CD8+ cells predominated over CD4+ cells. High numbers of CDllc+ macrophages were found in 4 out of 19 patients with regional disease before treatment. In the group with general disease high numbers were found in 5 out of 20 tumours. The occurence of inflammtory cells in fine needle as¬ pirates and in biopsies after IFN-α treatment were compared in 20 patients. Even if IFN-α might influence the recruit¬ ment of these cells there was in general a very good corre¬ lation. Lower numbers of CD4+ cells in tissue sections com¬ pared to aspirates were found in only 3 out of 20 patients. One of these 3 patients was a responder. All biopsies con- tained varying degrees of remnants of normal lymph node, which might explain this discrepancy. Alternatively a down regulation might have occured during IFN-α treatment. Two biopsies showed an increase of CD4+ cells compared to aspi¬ rates, one of them was a responder. The size of lymph node metastases seems to be of some guidance as to the prescence of remaining normal lymph node. Remnants of normal lymph node was found in 7 out of 13 lymph node metastases less than 20 mm in diameter in contrast to only 2 out of 7 in lymph node metastases of larger size. Correlation to response to IFN-α treatment. The occurence of inflammatory cells in relation to the therapeutic effect of IFN-α is shown in Table 2 for pa¬ tients with general metastases and in Table 3 for patients with regional, resectable metastases. Obviously, there is a very good correlation between anti-tumour effect and the presence of CD4+ lymphocytes in both these groups of pati¬ ents. The occurence of these cells in aspirates from res¬ ponding patients with distant metastases differs signifi¬ cantly from that of patients with progressive tumours (p = 0.007) . A similar difference is found also for patients with regional metastases, with more CD4+ lymphocytes in me¬ tastases shewing marked histopathological regressive changes after IFN-α treatment compared to patients with only slight or no regressive changes in the metastases (p = 0.077) . Surprisingly, there was no correlation between the occurence of CD8+ or CD11C+ cells and response to IFN-α treatment, neither in regional nor in general disease. Table 2. Number of tumour infiltrating CD4+, CD8+ and CDllc+ inflamatory cells in patients with inoperable systemic me¬ tastatic disease according to clinical effect of IFN-α treatment. Number of CD4 CD8 CDllc infiltrating Therapeutic ef- Therapeutic ef- Therapeutic ef- cells feet feet feet R MR PD R MR PD R MR PD Low 1 0 9 4 2 8 3 1 3 Moderate 4 2 1 1 1 2 3 1 4 High 3 1 0 2 0 0 1 1 3 Table 3. Number of tumour infiltrating CD4+, CD8+ and CDllc+ inflamatory cells in patients with regional disease accor¬ ding to histopathological regression after IFN-α treatment Number of CD4 CD8 M5 infiltrating cells Marked Slight Marked Slight Marked Slight histopat. regr. histopat. regr. histopat. regr. Low 1 5 3 6 2 4 Moderate 1 3 3 4 4 4 High 7 3 2 1 2 3 According to the present invention a good correlation was obtained between the occurrence of a specific class of cells and the therapeutic response to a drug against a tumour. In the example given this correlation was obtained with the helper T subset and the therapeutic benefit of IFN-α. In patients with regional disease 7 out of 10 pati¬ ents with high numbers of these cells in fine needle aspirates achieved marked histopathological tumour regres- sion. In contrast, in patients with low numbers of cells of this subset 5 out of 6 patients achieved only a slight re¬ gression of their tumours. In the group with distant meta¬ stases 10 out of 11 of those with moderate or high infilt¬ ration of cells of this subset in the tumours achieved tumour regression. In contrast, among patients with low in¬ filtration of these cells 9 out of 10 had progressive di¬ sease. Based on these results there seems to be a need for cells of this subset infiltrating the tumours before start of treatment with IFN-α to make the treatment successful. Thus, the degree of infiltration of these cells seems to be a useful predicitve test for chosing patients suitable for this therapy. In order to simplify and facilitate the diagnostic techniques with a fine needle aspirate of a tumour from a patient the use of a diagnostic kit is applied. An example of such a kit is given below. A single cell suspension the fine needle aspirate is prepared by suspending the aspirate in a solution containing a mixture of the enzymes collagenase, hyalu- ronidase and DNAase. A mixture of monoclonal antibodies directed towards tumour associated antigens on tumour cells and labelled with one type of fluorochrome (type A) is added to the cell suspension thus obtained. Then a mixture of monoclonal antibodies directed towards CD3 (a T-lympho- • cyte marker) and CD4 antigens on lymphocytes is added, i.e. anti-CD3 antibodies labelled with a fluorochrome type B and anti-CD4 antibodies labelled with a fluorochrome type C. Such a double staining is used in order to identify CD4+ lymphocytes and avoid the counting of CD4+ monocytes/macro- phages. The numbers of tumour cells and double stained lymphocytes are then determined by means of flow cytometry. A high ratio CD4+ lymphocytes/tumour cells will according to the invention identify responders to immunotherapy. References. Abershold, P. Lysis of autologous melanoma cells by tumour infiltrating lymphocytes: Association with clinical res- ponse. J Nat Cancer Inst. 1991;83:932-937. Balch, CM. A multifactorial analysis of melanoma: Prognos¬ tic Histopathological features comparing Clark's and Breslow's staging methods. Ann Surg. 1978;188:732-742. Balkwill,F. Human interferon inhibits the growth of estab- lished human breast tumours in the nude mouse . Int J Cancer. 1982;30:231-235. Breard, J. A monoclonal antibody reactive with human pe¬ ripheral blood monocytes. J Immunol. 1980;124 :1943. Cosimi, A. Use of monoclonal antibodies to T-cell subsets for immunological monitoring and treatment in recipients of renal allografts. N Eng J Med. 1981;305-308. Creagan, E. Recombinant Interferons in the management of Advanced malignant melanoma. Am J Clin Oncol. 1988;11: 652- 659. Friedman, R.M. Interferons with special emphasis on the im¬ mune system. Adv. Immunol. 1983;34:97-140. Gundersen, S. Interferon in combination with Vinblastin in advanced malignant melanoma. A phase I-II study. Cancer. 1989;64:1617-1619. Hansen, M.G. Tumour thickness and lymphocytic infiltration in malignant melanoma of the head and neck. Am J Surg. 1974;12:8557-8561. Hersey, P. Immunohistological relation between DR antigen expression on melanoma cells and infiltration by CD8+ T - cells. Pathology. 1990;22:133-139. Hutchinson, G.H. Differential immune reactivity of tumour intrinsic and peripheral blood lymphocytes against au- toplastic colorectal carcinoma cells. Br J Cancer. 1981;44 -.396-402. Kang, S. Histologic regression in malignant melanoma: an interobserver concordance study. J. Cutan. Pathol . 1993;126-129. Kirkwood, J. A randomized controlled trial of high-dose IFN-alpha-2b for high risk melanoma: The ECOG trial EST 1684. Proc Am Soc Clin Oncol 1993;12:390. Klein, E. Separation and characteristics of tumour infilt¬ rating lymphocytes in man. Immunobiology. 1980;10:79-107. Kornstein, M.J. Immunoperoxidase localization of lymphocyte subsets in the host respons to melanoma and nevi . Cancer Res. 1983;43:2749-2753. Larsen, T. A retrospective histological study of 669 cases of primary cutaneous malignant melanoma in clinical stage I. ACTA Pathol. Microbiol. Scand. 1978;86:523-530. Legha, S. Current therapy for malignant melanoma. Sem Oncol. 1989;16:34-44. Mc Govern, V.J. Lymphocytic infiltration and survival in malignant melanoma. Pathology malignant melanoma, A.B. Ackerman (ed.) , Masson Publishing. 1981,-341-344. Mc Govern, V.J. Spontaneous regression of melanoma. Pathology. 1975;7:91-99. Miescher, S. Functional properties of tumour infiltrating and blood lymphocytes in patients with solid tumours: Ef¬ fects of tumour cells and their supematants on prolifera- tive responses of lymphocytes. J Immunol. 1986;136 : 1899- 1907. Miescher, S. Preferential clonogenic deficit of CD8+ T lym¬ phocytes infiltrating human solid tumours. Cancer Res. Nind, A.P Lymphocyte anergy in patients with carcinoma. Br J Cancer. 1973;28:108-117. Nind, A. Lymphocyte anergy in patients with carcinoma. Br J Cancer. 1973;28:108-117. Onishi, T. Assessment of tumour infiltrating lymphocytes, regional lymph node lymphocytes and peripheral blood lym- phocytes and their reaction to Interferon-gamma in patients with renal cell carcinoma. Br J Urology. 1991;67:459-466. Poppema, S. In situ analysis of the mononuclear cell in¬ filtrate in primary malignant melanoma of the skin. Clin Exp immunol. 1983;51:77-82. Riviere, Y. Direct action of Interferon and inducers of in¬ terferon on tumour cells in athymic nude mice. Cancer Res. 1983;43:4596-4599. Ronan, S. Thin malignant melanomas with regression and me- tastases. Arch. Dermatol. 1987;123:1326-1330. Rosenberg, S.A. A new approach to the adoptive immunothe¬ rapy of cancer with tumour infiltrating lymphocytes. Science. 1986;233:1318-1321. Rosenberg, S.A. The immunotherapy and gene therapy of cancer. J Clin Oncol. 199." ;10 :188-199. Ruiter. Major histocompatibility antigens and mononuclear inflammatory infiltrate in benign nevomelanocytic prolife¬ rations and malignant melanoma. J Immunol. 1982 ;129 :2808- 2815. Sondergaard, K. Patrial regression in thin primary cutane¬ ous melignant melanomas clinical stage I. Virchows Arch. 1985;408:241-247. Tefany, F. Immunocytochemical analysis of the cellular in¬ filtrate in primary regressing and non-regressing malignant melanoma. Vose, B.M. Human tumour infiltrating lymphocytes: A marker of host response. Sem. Haematol . 1985;22:27-40. Vose, B.M. Mitogen responsiveness and inhibitory activity of mesenteric lymph node cells. Conditioned medium contai- • ning T cell growth factor reverses suppressor function. Cancer Immunol Immunother. 1982;13:105-111. Vose, B.M. Spe¬ cific and non-specific lymphocyte cytotoxicity in colon carcinoma. Br J Cancer. 1981;44:846-855.

Claims: CLAIMS 1. Method of predicting the therapeutic response of a drug against a malignant tumour in a human patient, using a sample of a fine needle aspirate of said tumour from said patient, c h a r a c t e r i z e d in that the method comprises the steps of identifying and estimating in said sample the number of cells belonging to one class of cells in relation to other cells in said sample; and comparing said estimation with a previously made analysis demonstrating correlation between the number of said cells and parameters of the therapeutic response of said drug di¬ rected towards said tumour in human patients; the results of said comparison being indicative of the the¬ rapeutic response of said drug in said human patient. 2. Method as in claim 1, c h a r a c t e r i z e d in that said drug is an immunomodulating drug. 3. Method as in claim 1, c h a r a c t e r i z e d in that said class of cells is a subset of tumour infiltrating mononuclear cells. 4. Method as in claim 3, c h a r a c t e r i z e d in that said subset of tumour infiltrating mononuclear cells is the helper T subset. 5. Method as in claim 1, c h a r a c t e r i z e d in that said tumour is a metastatic malignant melanoma and said drug is interferon-alfa. 6. Method as in claim 1, c h a r a c t e r i z e d in that said cells are identified and estimated by means of - immunological staining. 7. Method as in claim 6, c h a r a c t e r i z e d in that said immunological staining is performed with mono- klonal antibodies. 8. Diagnostic kit useful for identifying responders to immunotherapy when predicting the therapeutic response of a drug directed towards a malignant tumour in a human patient, c h a r a c t e r i z e d in that said kit comprises a mixture of enzymes rendering a sample of a fine needle aspirate of said tumour from said patient a single cell suspension, a first mixture of monoclonal antibodies directed towards tumour associated antigens on tumour cells in said single cell suspension, said antibodies having a first label, and a second mixture of two types of monoclonal antibodies directed towards two specific antigens on lymphocytes, said two types of antibodies having a second and a third label, respectively, the numbers of tumour cells and double stained lymphocytes being prepared for determination by blood counting. 9. Diagnostic kit as in claim 8, c h a r a c t e r ¬ i z e d in that said mixture of enzymes comprises collagenase, hyaluronidase and DNAase. 10. Diagnostic kit as in claim 8, c h a r a c t e r ¬ i z e d in that said two specific antigens on lymphocytes are CD3 and CD4 antigens. 11. Diagnostic kit as in claim 8, c h a r a c t e r - i z e d in that said first label is a fluorochrome type A, and said second label and said third label is a fluorochrome type B and C, respectively.

Inventors: GUSTAFSSON BERTIL, HAAKANSSON ANNIKA, HAAKANSSON LEIF, GUSTAFSSON, BERTIL, HAAKANSSON, ANNIKA, HAAKANSSON, LEIF
Applicants: GUSTAFSSON BERTIL, HAAKANSSON ANNIKA, HAAKANSSON LEIF, LANDSTINGET I OESTERGOETLAND, GUSTAFSSON, BERTIL, HAAKANSSON, ANNIKA, HAAKANSSON, LEIF
Application year: 1996
Main classification: G01N33
IPC codes: G01N33