VECTOR FOR EXPRESSION OF N-TERMINALLY EXTENDED PROTEINS IN YEAST CELL

Abstract: The present invention relates to a DNA construct comprising a DNA sequence encoding N-terminally extended protein polypeptides, vectors carrying such DNA fragments and yeast cells transformed with the vectors, as well as a process of producing heterologous proteins in eukaryotic cells including yeast. The DNA construct encodes the following structure: signal peptide-leader peptide-EEAEPK-polypeptide.

Description: Vector for expression of N-termiπally extended proteins in yeast cell . FIELD OF INVENTION The present invention relates to polypeptides expressed and processed in a eucary¬ otic cell, preferably a yeast cell, a DNA construct comprising a DNA sequence en¬ coding such polypeptides, vectors carrying such DNA fragments and cells including yeast cells transformed with the vectors, as well as a process of producing heterolo¬ gous proteins in yeast BACKGROUND OF THE INVENTION Yeast organisms produce a number of proteins synthesized intracellularly, but hav¬ ing a function outside the cell Such extracellular proteins are referred to as secreted proteins These secreted proteins are expressed initially inside the cell in a precur¬ sor or a pre-for m containing a presequence ensuring effective direction of the ex¬ pressed product across the membrane of the endoplasmatic reticulum (ER) The presequence, normally named a signal peptide, is generally cleaved off from the de¬ sired product during translocation Once entered in the secretory pathway, the pro- tein is transported to the Golgi apparatus From the Golgi the protein can follow dif¬ ferent routes that lead to compartments such as the cell vacuole or the cell mem¬ brane, or it can be routed out of the cell to be secreted to the external medium (Pfeffer, S R and Rothman, J E Ann Rev Biochem 56 (1987), 829-852 Several approaches have been suggested for the expression and secretion in yeast of proteins heterologous to yeast European Publication No 0088632A describes a process by which proteins heterologous to yeast are expressed, processed and se¬ creted by transforming a yeast organism with an expression vector harbouring DNA encoding the desired protein and a signal peptide, prepatmg a culture of the trans- formed organism, growing the culture and recovering the protein from the culture medium The signal peptide may be the desired protein's own signal peptide, a het¬ erologous signal peptide or a hybrid of a native and a heterologous signal peptide A problem encountered with the use of signal peptides heterologous to yeast might be that the heterologous signal peptide does not ensure efficient translocation and/or cleavage of the precursor polypeptide after the signal peptide The Saccharomyces cerevisiae MFα1 (α-factor) is synthesized as a prepro form of 165 ammo acids comprising a 19 am o acids long signal- or prepeptide followed by a 64 ammo acids long "leader" or propeptide, encompassing three N-linked glyco¬ sylation sites followed by (LysArg(Asp/Glu, Ala) 2-3 α-factor) 4 (Kurjan, J and Herskow- itz, I Cell 30 (1982), 933-943) The signal-leader part of the preproMFαl has been widely employed to obtain synthesis and secretion of heterologous proteins in S cerevisiae Use of signal/leader peptides homologous to yeast is known from i a US Patent No 4,546,082, European Publications Nos 0116201 A, 0123294A, 0123544A, 0163529A, 0123289A and European Patent No 0100561 B In EP 0123289A utilization of the S cerevisiae α-factor precursor is described whereas EP 100561 describes the utilization of the Saccharomyces cerevisiae PH05 signal and PCT Publication No WO 95/02059 describes the utilization of YAP3 sig¬ nal peptide for secretion of foreign proteins US Patent No 4,546,082 and European Publications Nos 0016201A, 0123294A, 0123544A and 0163529A describe processes by which the α-factor signal-leader from Saccharomyces cerevisiae (MFα1 or MFα2) is utilized in the secretion process of expressed heterologous proteins in yeast By fusing a DNA sequence encoding the S cerevisiae MFα1 signal/leader sequence at the 5' end of the gene for the de- sired protein secretion and processing of the desired protein was demonstrated EP 206783 discloses a system for the secretion of polypeptides from S cerevisiae whereby the α-factor leader sequence has been truncated to eliminate the four α- factor peptides present on the native leader sequence so as to leave the leader peptide itself fused to a heterologous polypeptide via the α-factor processing site Lys-Ar g-Glu-Ala-Glu-Ala This construction is indicated to lead to an efficient proc¬ ess for production of smaller peptides (less than 50 ammo acids) For the secretion and processing of larger polypeptides, the native α-factor leader sequence has been truncated to leave one or two α-factor peptides between the leader peptide and the polypeptide A number of secreted proteins are routed so as to be exposed to a proteolytic proc¬ essing system which can cleave the peptide bond at the carboxy end the two con¬ secutive basic ammo acids This enzymatic activity is in S cerevisiae encoded by the KEX 2 gene (Julius, D A et al , Cell 37 (1984b), 1075) Processing of the prod- uct by the KEX 2 protease is needed for the secretion of active S cerevisiae mating factor α1 (MFα1 or α-factor) but is not involved in the secretion of active S cere¬ visiae mating factor a Secretion and correct processing of a polypeptide intended to be secreted is ob- tamed in some cases when culturing a yeast organism which is transformed with a vector constructed as indicated in the references given above In many cases, how¬ ever, the level of secretion is very low or there is no secretion, or the proteolytic processing may be incorrect or incomplete As described in PCT Publication No WO 90/10075 this is believed to be ascπbable, to some extent, to an insufficient expo- sure of the processing site present between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to render it inaccessible or, at least, less accessible to proteolytic cleavage WO 90/10075 describes a yeast expression system with improved processing of a heterologous polypeptide obtained by providing certain modifications near the proc¬ essing site at the C-terminal end of the leader peptide and/or the N-terminal end of a heterologous polypeptide fused to the leader peptide Thus, it is possible to obtain a higher yield of the correctly processed protein than is obtainable with unmodified leader peptide-heterologous polypeptide constructions It is the object of the present invention to provide a novel N-terminal modification of a heterologous polypeptide fused to the leader peptide which ensures a more efficient expression and/or proc¬ essing as well as improved removal in vitro of the N-terminal extension of heterolo¬ gous polypeptides SUMMARY OF THE INVENTION The present invention describes modifications of the N-terminal end of the heterolo¬ gous polypeptide designed as extensions which can be cleaved off by in vitro prote- olysis during or subsequently to purification of the product from the culture media The present invention relates to a DNA construct encoding a polypeptide having the following structure signal peptide-ieader peptide-EEAEPK-heterologous protein The conventional one-letter code for ammo acids in accordance with rules approved (1974) by the IUPAC-IUB Commission of Biochemical Nomenclature is used throughout this description and in the claims In the present context, the term "signal peptide" is understood to mean a prese- quence which is present as an N-terminal sequence on the precursor form of an ex¬ tracellular protein expressed in yeast The function of the signal peptide is to allow the heterologous protein to be secreted to enter the endoplasmatic reticulum The signal peptide is normally cleaved off in the course of this process The signal pep¬ tide may be heterologous or homologous to the yeast organism producing the pro- tein A preferred signal peptide in this invention is yeast aspartic protease 3 (YAP3) signal peptide or any functional analogue thereof YAP 3 has been cloned and char¬ acterised by Egel-Mitani, M et al , YEAST, 6, p 127-137, 1990 The expression "leader peptide" is understood to indicate a peptide in the form of a propeptide sequence whose function is to allow the heterologous protein to be se¬ creted to be directed from the endoplasmatic reticulum to the Golgi apparatus and further to a secretory vesicle for secretion into the medium, (i e exportation of the expressed protein or polypeptide across the cellular membrane and cell wall, if pres¬ ent, or at least through the cellular membrane into the peπplasmic space of a cell having a cell wall) Preferably the leader peptide is the LA19 leader peptide dis¬ closed as SEQ ID No 5 herein The expression "heterologous protein" is intended to indicate a protein or polypep¬ tide which is not produced by the host organism in nature, preferably a polypeptide which is not produced by yeast in nature However, it would be obvious to a person skilled in the art to produce homologous polypeptides, such as glucagon in a mam¬ malian cell, and said polypeptides are included in the meaning of "heterologous protein" The sequence EEAEPK forms an extension at the N-terminal of the heterologous polypeptide This extension not only increases the fermentation yield but is protected against dipeptidyl aminopeptidase (DPAP A) processing resulting in a homogenous N-terminal of the polypeptide The extension is constructed in such a way that it is resistant to proteolytic cleavage during fermentation so that the N-terminally ex- tended heterologous protein product can be purified from the culture media for sub¬ sequent in vitro maturation, e g by trypsin or Achromobacter lyticus protease I The desired in vitro removal of the N-terminal extension EEAEPK is readily achieved by either trypsin or Achromobacter lyticus protease I presumably due to flexibility of the N-terminal extension peptide resulting in an improved yield of the matured heterolo- gous protein The invention further relates to a recombinant expression vector which is capable of replicating in a eucaryotic cell, preferably a yeast cell, and which carries a DNA construct of the invention Preferably, the DNA construct comprises a synthetic leader peptide, preferably the LA19 leader peptide Besides, the invention relates to the DNA construct described in Fig 2 herein The invention also relates to a eucaryotic cell, preferably a yeast cell, which is capable of expressing a heterologous protein and which is transformed with a vector of the invention Furthermore, the invention relates to a process for producing a heterologous protein in yeast, comprising cultivating the transformed yeast strain in a suitable medium to obtain expression and secretion of the heterologous protein, after which the protein is isolated from the medium and/or the cells BRIEF DESCRIPTION OF THE DRAWINGS The present invention is further illustrated with reference to the appended drawings, wherein Fig 1 shows the expression plasmid pAK729 containing genes expressing the N- terminally extended polypeptides of the invention, and the following symbols are used TPI-PROMOTER Denotes the TPI gene promoter sequence from S cerevisiae 2 Denotes the region encoding a signal/leader peptide (e g from the YAP3 signal peptide and LA19 leader peptide in conjunction with the EEAEPK N-terminally ex¬ tended MI3 insulin precursor TPI-TERMINATOR Denotes TPI gene terminator sequence of S cerevisiae TPI-POMBE Denotes TPI gene from S pombe Origin Denotes a sequence from S cerevisiae 2 μ plasmid including its origin of DNA replication in S cerevisiae AMP-R Sequence from pBR322 /pUC13 including the ampicillin resistance gene and an origin of DNA replication in E coli SEQ ID NO 1 is the DNA and ammo acid sequences in pAK729 encoding the YAP3 signal peptide (am o acid No 1 through 21 ), LA19 leader peptide (ammo acid No 22 through 64), N-terminal extension EEAEPK (am o acid No 65 through 70), MI3 insulin precursor B chain (1 -29)-Ala-Ala-Lys-A c haιn(1 -21 ) (am o acid No 71 through 123) SEQ ID NO 2 is the DNA and am o acid sequences in pAK733 encoding the YAP3 signal petιde-LA19 leader EEAEPK-MI1 insulin precursor complex of Example 2 SEQ ID NO 3 is the DNA and ammo acid sequences in pAK749 encoding the YAP3 signal petιde-LA19 leader EEAEPK-MI5 insulin precursor complex of Example 3 SEQ ID NO 4 is the DNA and am o acid sequences in pAK866 encoding the YAP3 signal petιde-LA19 leader EEAEPK-X14 insulin precursor complex of Example 4 SEQ ID NO 5 is the LA19 leader DNA sequence DETAILED DESCRIPTION OF THE INVENTION The N-terminally extended heterologous protein produced by the method of the in¬ vention may be any protein which may advantageously be produced in a eucaryotic cell, preferably a yeast cell Examples of such proteins are aprotinin, tissue factor pathway inhibitor or other protease inhibitors, and insulin or insulin precursors, insu¬ lin analogues, insulin-like growth factor I or II, human or bovine growth hormone, - terleukin, tissue plasminogen activator, transforming growth factor a or b, glucagon, glucagon-like peptide 1 (GLP-1 ), glucagon-like peptide 2 (GLP-2), GRPP, Factor Vll, Factor VIII, Factor XIII, platelet-derived growth factor, enzymes, such as lipases, or a functional analogue of any one of these proteins In the present context, the term "functional analogue" is meant to indicate a polypeptide with a similar function as the native protein (this is intended to be understood as relating to the nature rather than the level of biological activity of the native protein) The polypeptide may be struc¬ turally similar to the native protein and may be derived from the native protein by ad- dition of one or more am o acids to either or both the C- and N-terminal end of the native protein, substitution of one or more ammo acids at one or a number of differ¬ ent sited in the native am o acid sequence deletion of one or more am o acids at either or both ends of the native protein or at one or several sites in the ammo acid sequence, or insertion of one or more ammo acids at one or more sites in the native ammo acid sequence Such modifications are well known for several of the proteins mentioned above By "a precursor of insulin" or "a precursor an insulin analogue" is to be understood a single-chain polypeptide, including proinsulin, which by one or more subsequent chemical and/or enzymatical processes can be converted to a two-chain insulin or insulin analogue molecule having the correct establishment of the three disulphide bridges as found in natural human insulin Examples of insulins or insulin analogues which can be produced in this way are human insulin, preferably des(B30) human insulin, porcine insulin, and insulin analogues wherein at least one Lys or Arg is present, preferably insulin analogues wherein Phe B1 has been deleted, insulin analogues wherein the A-chain and/or the B-chain have an N-terminal extension and insulin analogues wherein the A-chain and/or the B-chain have a C-terminal extension Other preferred insulin analogues are such wherein one or more of the ammo acid residues, preferably one, two, or three of them, have been substituted by another codable am o acid residue Thus in position A21 a parent insulin may instead of Asn have an am o acid residue selected from the group comprising Ala, Gin, Glu, Gly, His, lie, Leu, Met, Ser, Thr, Trp, Tyr or Val, in particular an am o acid residue selected from the group comprising Gly, Ala, Ser, and Thr The insulin analogues may also be modified by a combination of the changes outlined above Likewise, in position B28 a parent insulin may instead of Pro have an ammo acid residue selected from the group comprising Asp, Lys etc , and in position B29 a parent insulin may instead of Lys have the ammo acid Pro The expression "a codable am o acid residue" as used herein designates an ammo acid residue which can be coded for by the genetic code, i e a triplet ("codon") of nucleotides Most preferred insulin precursors are MM , B(1-29)-A( 1-21 ), MI3, B(1 -29)-Ala-Ala- Lys-A(1-21 ) (as described m e g EP 163 529), X14, B(1 -27-Asp-Lys)-Ala-Ala-Lys- A(1-21 ) (as described m e g PCT publication No 95/00550), B(1-27-Asp-Lys)-A(1 - 21 ), B(1-27-Asρ-Lys)-Ser-Asp-Asp-Ala-Lys-A(1 -21 ), B(1 -29)-Ala-Ala-Arg-A(1-21 ) (as described e g PCT Publication No 95/07931 ), MI5, B(1-29)-Ser-Asp-Asp-Ala-Lys- A(1 -21 ), and B(1-29)-Ser-Asp-Asp-Ala-Arg-A(1-21 ) The DNA construct of the invention encoding the polypeptide of the invention may be prepared synthetically by established standard methods, e g the phosphoamidite method described by S L Beaucage and M H Caruthers, Tetrahedron Letters 22. 1981 , pp 1859-1869, or the method described by Matthes et al , EMBO Journal 3. 1984, pp 801-805 According to the phosphoamidite method, oiigonucleotides are synthesized, e g in an automatic DNA synthesizer, purified, duplexed and ligated to form the synthetic DNA construct A currently preferred way of preparing the DNA construct is by polymerase chain reaction (PCR), e g as described in Sambrook et al , Molecular Cloning A Laboratory Manual. Cold Spring Harbor, NY, 1989) The DNA construct of the invention may also be of genomic or cDNA origin, for in¬ stance obtained by preparing a genomic or cDNA library and screening for DNA se¬ quences coding for all or part of the polypeptide or the invention by hybridization using synthetic oligonucleotide probes in accordance with standard techniques (cf Sambrook et al , Molecular Cloning A Laboratory Manual, Cold Spring Harbor, 1989) In this case, a genomic or cDNA sequence encoding a signal and leader peptide may be joined to a genomic or cDNA sequence encoding the heterologous protein, after which the DNA sequence may be modified at a site corresponding to the am o acid sequence EEAEPK of the polypeptide, by inserting synthetic oiigonu¬ cleotides encoding the desired ammo acid sequence for homologous recombination in accordance with well-known procedures or preferably generating the desired se¬ quence by PCR using suitable oiigonucleotides Finally, the DNA construct may be of mixed synthetic and genomic, mixed synthetic and cDNA or mixed genomic and cDNA origin prepared by annealing fragments of synthetic, genomic or cDNA origin (as appropriate), the fragments corresponding to various parts of the entire DNA construct, in accordance with standard techniques Thus, it may be envisaged that the DNA sequence encoding the heterologous pro¬ tein may be of genomic or cDNA origin, while the sequence encoding the signal and leader peptide as well as the sequence encoding the N-terminal extension EEAEPK may be prepared synthetically In one aspect, the invention relates to a recombinant expression vector which is ca¬ pable of replicating in yeast and which carries a DNA construct encoding the above- defined polypeptide The recombinant expression vector may be any vector which is capable of replicating in yeast organisms In the vector, the DNA sequence encoding the polypeptide of the invention should be operably connected to a suitable promoter sequence The promoter may be any DNA sequence which shows transscπptional activity in yeast and may be derived from genes encoding proteins either homolo- gous or heterologous to yeast The promoter is preferably derived from a gene en¬ coding a protein homologous to yeast Examples of suitable promoters are the Sac¬ charomyces cerevisiae Mα1 , TPI, ADH or PGK promoters The DNA sequence encoding the polypeptide of the invention may also be operably connected to a suitable terminator, e g the TPI terminator (cf T Alber and G Kawa¬ saki, J Mol Appl Genet 1. 1982, pp 419-434) The recombinant expression vector of the invention compπses a DNA sequence enabling the vector to replicate in yeast Examples of such sequences are the yeast plasmid 2μ replication genes REP 1-3 and origin of replication The vector may also comprise a selectable marker, e g the Schizosaccharomyces pompe TPI gene as described by P R Russell, Gene 40, 1985, pp 125-130 The procedures used to ligate the DNA sequences coding for the polypeptide of the invention, the promoter and the terminator, respectively, and to insert them into suit¬ able yeast vectors containing the information necessary for yeast replication, are well known to persons skilled in the art (cf , for instance, Sambrook et al , op cit ) It will be understood that the vector may be constructed either by first preparing a DNA construct containing the entire DNA sequence coding for the polypeptide of the in- vention and subsequently inserting this fragment into a suitable expression vector, or by sequentially inserting DNA fragments containing genetic information for the indi¬ vidual elements (such as the signal, leader or heterologous protein) followed by ligation The yeast organism or eucaryotic host cell used in the process of the invention may be any suitable yeast organism which, on cultivation, produces large amounts of the heterologous protein or polypeptide in question Examples of suitable yeast organ¬ isms may be strains selected from the yeast species Saccharomyces cerevisiae, Saccharomyces kluyveπ, Schizosaccharomyces pombe, Saccharomyces uvarum, Kluyveromyces lactis, Hansenula polymorpha, Pichia pastoris, Pichia methanolica, Pichia kluyveπ, Yarrowia lipolytica, Candida sp , Candida utilis, Candida cacaoi, Geo- tπchum sp , and Geotπchum fermentans, preferably the yeast species Saccharomyces cerevisiae It is considered obvious for the skilled person in the art to select any other eucaryotic cell, such as cells of the genera Aspergillus, Streptomyces, insect cells or a mammalian cell, as the host organism The transformation of the yeast cells may for instance be effected by protoplast for¬ mation followed by transformation in a manner known per se The medium used to cultivate the cells may be any conventional medium suitable for growing yeast or- ganisms The secreted heterologous protein, a significant proportion of which will be present in the medium in correctly processed form, may be recovered from the me- dium by conventional precedures including separating the yeast cells from the me¬ dium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt, e g ammonium sulphate, followed by puri¬ fication by a variety of chromatographic procedures, e g ion exchange chromatog¬ raphy, affinity chromatography, or the like When the protein is secreted to the pe- πplasmic space, the cells are disrupted enzymatically or mechanically After secretion to the culture medium or the peπplasmic space, the protein may be subjected to vaπous procedures to remove the sequence EEAEPK The extension is found to be stably attached to the heterologous protein during fer¬ mentation, protecting the N-terminal of the heterologous protein against the prote¬ olytic activity of yeast proteases such as DPAP The presence of an N-terminal ex¬ tension on the heterologous protein may also serve as a protection of the N-termmal am o group of the heterologous protein during chemical processing of the protein, i e it may serve as a substitute for a BOC or similar protecting group In such cases the ammo acid sequence EEAEPK may be removed from the recovered heterolo¬ gous protein by means of a proteolytic enzyme which is specific for a basic ammo acid (i e K (Lys)) so that the terminal extension is cleaved off at the K Examples of such proteolytic enzymes are trypsin or Achromobacter lyticus protease I The present invention is described in further detail in the following examples which are not in any way intended to limit the scope of the invention as claimed Exampfe 1. Construction of the yeast strain yAK729 expressing the EEAEPK- MI3 insulin precursor A synthetic gene coding for the N-terminal extension of N-terminally extended insulin precursor EEAEPK-MI3 was constructed using the Polymerase Cham Reaction (PCR) The following 2 oiigonucleotides were synthesized #672 5' -TCTCCATGGCTAAGAGAGAAGAAGCTGAACCAAAGTTCGTT-3' #2785 5' -AATTTATTTTACATAACACTAG-3' Oiigonucleotides were synthesized using an automatic DNA synthesizer (applied Biosystems model 380A) using phosphoamidite chemistry and commercially available reagents (Beaucage, S L and Caruther s, M H , Tetrahedron letters 22 (1981 ) 1859-1869) The following PCR was performed using the Pwo DNA Polymerase (Boehπnger Mannheim GmbH, Sandhoefter Strasse 116, Mannheim, Germany) according to the manufacturer's instructions and The PCR mix was overlayed with 100 μl mineral oil (Sigma Chemical Co , St Louis MO, USA) PCR 5 μl oligonucleotide #672 (50 pmol) 5 μl oligonucleotide #2785 (50 pmol) 10 μl 10X PCR buffer 0 5 μl Pwo enzyme 0 5 μl pAK680 plasmid as template (0 2 ug DNA) 71 μl dest water A total of 12 cycles were performed, one cycle was 94 C for 45 se , 40 C for 1 m , 72 C for 1 5 m The PCR mixture was then loaded onto a 2 5% agarose gel and electroforese was performed using standard techniques (Sambrook J, Fπtsch El and Maniatis T, Molecular cloning, Cold Spring harbour laboratory press, 1989) The resulting DNA fragment was cut out of the agarose gel and isolated by the Gene Clean Kit (Bio 101 mc , PO BOX 2284, La Jolla, CA 92038, USA) according to the manufacturer's instructions The purified PCR DNA fragment was dissolved in 14 μl of water and restriction endonucleases buffer and cut with the restriction endonucleases Ncol and Xbal according to standard techniques (Sambrook J, Fntsch EF and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989) The Ncol-Xbal DNA fragment on 209 nucleotide base pairs was subjected to agarose electroforese and purified using The Gene Clean Kit as described The plasmid pAK721 (see Fig 1 ) was cut with the restriction endonucleases Bglll and Xbal and the vector fragment of 10849 nucleotide base pairs isolated using The Gene Clean Kit as described The plasmid pAK721 was cut with the restriction endonucleases Bglll and Ncol and the DNA fragment of 160 nucleotide base pairs isolated using The Gene Clean Kit as described The three DNA fragments was ligated together using T4 DNA ligase and standard conditions (Sambrook J, Fntsch EF and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989) The ligation mix was then transformed into a competent E coli strain (R-, M+) followed by selection with ampicillin resistance Plasmid from the resulting E coli was isolated using standard techniques (Sambrook J, Fntsch EF and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989), and checked for insert with appropriate restriction endonucleases i e Neo I and Xbal The selected plasmid was shown by DNA sequence analyse (Sequenase, U S Biochemical Corp , USA) to encode the correct DNA sequence for the EEAEPK-MI3 insulin precursor DNA and to be inserted after the DNA encoding the synthetic LA19 leader DNA The plasmid was named pAK729 The DNA sequence encoding the YAP3 signal peptιde-LA19 leader EEAEPK-MI3 insulin precursor complex is shown in SEQ ID NO 1 The plasmid pAK721 was transformed into S cerevisiae strain MT663 as described in PCT/DK95/00250 and the resulting strain was named yAK721 The yeast expression plasmid pAK729 is of the C-POT type (see Fig 1 ) and is similar to those described in WO EP 171 142, which contain the Schizosaccharo¬ myces pombe tπose phosphate isomerase gene (POT) for plasmid selection and stabilisation in S cerevisiae pAK729 also contain the S cerevisiae tπose phosphate isomerase promoter and terminator The promoter and terminator are similar to those described in the plasmid pKFN1003 (described in WO 90/100075) as are all sequences in plasmid except the sequence between the EcoRIXbal fragment encoding the YAP3 signal peptιde-LA19 leader peptιde-EEAEPK-MI3 insulin precursor Example 2. Construction of the yeast strain yAK733 expressing the EEAEPK- MM insulin precursor A synthetic gene coding for the N-terminal extension of N-termιnally extended insulin precursor EEAEPK-MI1 was constructed by combining various DNA fragment en¬ coding leader and extension and insulin precursor The plasmid pAK721 ( see Fig 1 ) was cut with the restriction endonucleases Bglll and Xbal and the vector fragment of 10849 nucleotide basepars isolated using The Gene Clean Kit as described The plasmid pAK730 was cut with the restriction endonucleases Hmdlll and Xbal and the DNA fragment of 131 nucleotide basepars isolated using The Gene Clean Kit as described The plasmid pAK729 was cut with the restriction endonucleases Bglll and Hmdlll and the DNA fragment of 229 nucleotide was isolated as described above The three DNA fragments was ligated together using T4 DNA ligase and standard conditions (Sambrook J, Fntsch EF and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989) The ligation mix was then transformed into a com¬ petent E coli strain (R-, M+) followed by selection with ampicillin resistance Plasmid from the resulting E coli was isolated using standard techniques (Sambrook J, Fntsch EL and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989), and checked for insert with appropriate restrictions endonucleases The se¬ lected plasmid was shown by DNA sequence analyse (Sequenase, U S Biochemical Corp , USA) to encode the correct DNA sequence for the EEAEPK-MI1 insulin pre¬ cursor DNA and to be inserted after the DNA encoding the synthetic LA19 leader and extension DNA The plasmid was named pAK733 The DNA sequence encoding the YAP3 signal petιde-LA19 leader EEAEPK-MI1 in¬ sulin precursor complex is shown in SEQ ID NO 2 The plasmid pAK733 was transformed into S cerevisiae strain MT663 as described in PCT/DK95/00250 and the resulting strain was named yAK733 The yeast expres¬ sion plasmid pAK733 is of the C-POT type (see Fig 1 ) and is similar to those de¬ scribed in WO EP 171 142, which contains the Schizosaccharomyces pombe tπose phosphate isomerase gene (POT) for plasmid selection and stabilisation in S cerevisiae pAK733 also contain the S cerevisiae tπose phosphate isomerase promoter and terminator The promoter and terminator are similar to those de¬ scribed in the plasmid pKFN1003 (described in WO 90/100075) as are all se¬ quences in plasmid except the sequence between the EcoRI-Xbal fragment encod¬ ing the YAP3 signal peptide- LA19 leader-EEAEPK-MI1 insulin precursor Example 3. Construction of the yeast strain yAK749 expressing the EEAEPK- MI5 insulin precursor A synthetic gene coding for the N-terminal extension of N-terminally extended insulin precursor EEAEPK-MI5 was constructed by combining various DNA fragment en- coding leader and extension and insulin precursor The plasmid pAK743 was cut with the restriction endonucleases Bglll and Nhel and the vector fragment of 10757 nucleotide basepars isolated using The Gene Clean Kit as described The plasmid ρAK405 was cut with the restriction endonucleases Hindlll and Nhel and the DNA fragment of 238 nucleotide basepars isolated using The Gene Clean Kit as described The plasmid pAK729 was cut with the restriction endonucleases Bglll and Hindlll and the DNA fragment of 229 nucleotide was isolated as described above The three DNA fragments was ligated together using T4 DNA ligase and standard conditions (Sambrook J, Fntsch EF and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989) The ligation mix was then transformed into a com- petent E coli strain (R-, M+) followed by selection with ampicillin resistance Plasmid from the resulting E coli was isolated using standard techniques (Sambrook J, Fntsch EL and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989), and checked for insert with appropriate restrictions endonucleases The se¬ lected plasmid was shown by DNA sequence analyse (Sequenase, U S Biochemical Corp , USA) to encode the correct DNA sequence for the EEAEPK-MI5 insulin pre¬ cursor DNA and to be inserted after the DNA encoding the synthetic LA19 leader and extension DNA The plasmid was named pAK749 The DNA sequence encoding the YAP3 signal petιde-LA19 leader EEAEPK-MI5 - sulm precursor complex are shown in SEQ ID NO 3 The plasmid pAK749 was transformed into S cerevisiae strain MT663 as described in PCT/DK95/00250 and the resulting strain was named yAK749 The yeast expres¬ sion plasmid pAK749 is of the C-POT type (see Fig 1 ) and is similar to those de- scribed in WO EP 171 142, which contains the Schizosaccharomyces pombe tπose phosphate isomerase gene (POT) for plasmid selection and stabilisation in S. cerevisiae. pAK749 also contain the S. cerevisiae those phosphate isomerase promoter and terminator. The promoter and terminator are similar to those de¬ scribed in the plasmid pKFN1003 (described in WO 90/100075) as are all se¬ quences in plasmid except the sequence between the EcoRI-Xbal fragment encod- ing the YAP3 signal peptide- LA19 leader-EEAEPK-MI5 insulin precursor. Example 4. Construction of the yeast strain yAK866 expressing the EEAEPK- X14 insulin precursor A synthetic gene coding for the N-terminal extension of N-terminally extended insulin precursor EEAEPK-X14 was constructed by combining various DNA fragment en¬ coding leader and extension and X14 insulin precursor. The plasmid pAK743 was cut with the restriction endonucleases Bglll and Nhel and the vector fragment of 10757 nucleotide basepars isolated using The Gene Clean Kit as described. The plasmid pAK602 was cut with the restriction endonucleases Hindlll and Nhel and the DNA fragment of 232 nucleotide basepars isolated using The Gene Clean Kit as described. The plasmid pAK729 was cut with the restriction endonucleases Bglll and Hindlll and the DNA fragment of 229 nucleotide was isolated as described above. The three DNA fragments was ligated together using T4 DNA ligase and standard conditions (Sambrook J, Fritsch EF and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989). The ligation mix was then transformed into a com¬ petent E. coli strain (R-, M+) followed by selection with ampicillin resistance. Plasmid from the resulting E. coli was isolated using standard techniques (Sambrook J, Fritsch EL and Maniatis T, Molecular cloning, Cold spring Harbour laboratory press, 1989), and checked for insert with appropriate restrictions endonucleases. The se- lected plasmid was shown by DNA sequence analyse (Sequenase, U S Biochemical Corp , USA) to encode the correct DNA sequence for the EEAEPK-X14 insulin pre¬ cursor DNA and to be inserted after the DNA encoding the synthetic LA19 leader and extension DNA The plasmid was named pAK866 The DNA sequence encoding the YAP3 signal petide-LA19 leader EEAEPK-X14 in¬ sulin precursor complex are shown in SEQ ID NO 4. The plasmid pAK866 was transformed into S cerevisiae strain MT663 as described in PCT/DK95/00250 and the resulting strain was named yAK866 The yeast expres¬ sion plasmid pAK866 is of the C-POT type (see Fig 1 ) and is similar to those de¬ scribed in WO EP 171 142, which contains the Schizosaccharomyces pombe tπose phosphate isomerase gene (POT) for plasmid selection and stabilisation in S cerevisiae pAK866 also contain the S cerevisiae tπose phosphate isomerase promoter and terminator The promoter and terminator are similar to those de¬ scribed in the plasmid pKFN1003 (described in WO 90/100075) as are all se¬ quences in plasmid except the sequence between the EcoRI-Xbal fragment encod¬ ing the YAP3 signal peptιde-LA19 leader-EEAEPK-X14 insulin precursor SEQ ID NO 1 1 M K L K T V R S A V L S Bglll ATGAAA CTGAAAACTG TAAGATCTGC GGTCCTTTCG TACTTT GACTTTTGAC ATTCTAGACG CCAGGAAAGC S F A S Q V G Q P I D D T E S TCACTCTTTG CATCTCAGGT CCTTGGCCAA CCAATTGACG ACACTGAATC AGTGAGAAAC GTAGAGTCCA GGAACCGGTT GGTTAACTGC TGTGACTTAG N T T S V N L M A D D T E S R F Xbal TAACACTACT TCTGTCAACT TGATGGCTGA CGACACTGAA TCTAGATTCG ATTGTGATGA AGACAGTTGA ACTACCGACT GCTGTGACTT AGATCTAAGC A T N T T L A L D V V N L I S M A Ncol CTACTAACAC TACTTTGGCT TTGGATGTTG TTAACTTGAT CTCCATGGCT GATGATTGTG ATGAAACCGA AACCTACAAC AATTGAACTA GAGGTACCGA K R E E A E P K F V N Q H L C G S AAGAGAGAAG AAGCTGAACC AAAGTTCGTT AACCAACACT TGTGCGGTTC TTCTCTCTTC TTCGACTTGG TTTCAAGCAA TTGGTTGTGA ACACGCCAAG H L V E A L Y L V C G E R G F F CCACTTGGTT GAAGCTTTGT ACTTGGTTTG CGGTGAAAGA GGTTTCTTCT GGTGAACCAA CTTCGAAACA TGAACCAAAC GCCACTTTCT CCAAAGAAGA Y T P K A A K G I V E Q C C T S I ACACTCCTAA GGCTGCTAAG GGTATTGTCG AACAATGCTG TACCTCCATC TGTGAGGATT CCGACGATTC CCATAACAGC TTGTTACGAC ATGGAGGTAG 123 C S L Y Q L E N Y C N * TGCTCCTTGT ACCAATTGGA AAACTACTGC AACTAGACGC AGCCCGCAGG ACGAGGAACA TGGTTAACCT TTTGATGACG TTGATCTGCG TCGGGCGTCC Xbal CTCTAGA GAGATCT SEQ ID NO 2 EcoRI 901 TTCTTGCTTA AATCTATAAC TACAAAAAAC ACATACAGGA ATTCCATTCA AAGAACGAAT TTAGATATTG ATGTTTTTTG TGTATGTCCT TAAGGTAAGT 951 AGAATAGTTC AAACAAGAAG ATTACAAACT ATCAATTTCA TACACAATAT TCTTATCAAG TTTGTTCTTC TAATGTTTGA TAGTTAAAGT ATGTGTTATA +1 M K L K T V R S A V L S Bglll 1001 AAACGATTAA AAGAATGAAA CTGAAAACTG TAAGATCTGC GGTCCTTTCG TTTGCTAATT TTCTTACTTT GACTTTTGAC ATTCTAGACG CCAGGAAAGC + 1 S L F A S Q V L G Q P I D D T E S Styl 1051 TCACTCTTTG CATCTCAGGT CCTTGGCCAA CCAATTGACG ACACTGAATC AGTGAGAAAC GTAGAGTCCA GGAACCGGTT GGTTAACTGC TGTGACTTAG +1 N T T S V N L M A D D T E S R F Xbal 1101 TAACACTACT TCTGTCAACT TGATGGCTGA CGACACTGAA TCTAGATTCG ATTGTGATGA AGACAGTTGA ACTACCGACT GCTGTGACTT AGATCTAAGC +1 A T N D V V S M A Styl Ncol 1151 CTACTAACAC TACTTTGGCT TTGGATGTTG TTAACTTGAT CTCCATGGCT GATGATTGTG ATGAAACCGA AACCTACAAC AATTGAACTA GAGGTACCGA + 1 K R E E A E P K F V N Q H L C G S 1201 AAGAGAGAAG AAGCTGAACC AAAGTTCGTT AACCAACACT TGTGCGGTTC TTCTCTCTTC TTCGACTTGG TTTCAAGCAA TTGGTTGTGA ACACGCCAAG +1 H L V E A L Y L V C G E R G F F Hmdlll 1251 CCACTTGGTT GAAGCTTTGT ACTTGGTTTG CGGTGAAAGA GGTTTCTTCT GGTGAACCAA CTTCGAAACA TGAACCAAAC GCCACTTTCT CCAAAGAAGA + 1 Y T P K G I V E Q C C T S I C S L Bsu36I 1301 ACACTCCTAA GGGTATTGTC GAACAATGCT GTACCTCCAT CTGCTCCTTG TGTGAGGATT CCCATAACAG CTTGTTACGA CATGGAGGTA GACGAGGAAC + 1 Y Q L E N Y C N * Xbal 1351 TACCAATTGG AAAACTACTG CAACTAGACG CAGCCCGCAG GCTCTAGAAA ATGGTTAACC TTTTGATGAC GTTGATCTGC GTCGGGCGTC CGAGATCTTT SEQ ID NO 3. EcoRI 901 TTCTTGCTTA AATCTATAAC TACAAAAAAC ACATACAGGA ATTCCATTCA AAGAACGAAT TTAGATATTG ATGTTTTTTG TGTATGTCCT TAAGGTAAGT 951 AGAATAGTTC AAACAAGAAG ATTACAAACT ATCAATTTCA TACACAATAT TCTTATCAAG TTTGTTCTTC TAATGTTTGA TAGTTAAAGT ATGTGTTATA + 1 M K L K T V R S A V L S Bglll 1001 AAACGATTAA AAGAATGAAA CTGAAAACTG TAAGATCTGC GGTCCTTTCG TTTGCTAATT TTCTTACTTT GACTTTTGAC ATTCTAGACG CCAGGAAAGC +1 S L F A S Q V L G Q P I D D T E S Styl 1051 TCACTCTTTG CATCTCAGGT CCTTGGCCAA CCAATTGACG ACACTGAATC AGTGAGAAAC GTAGAGTCCA GGAACCGGTT GGTTAACTGC TGTGACTTAG +1 V N M A S R Xbal 1101 TAACACTACT TCTGTCAACT TGATGGCTGA CGACACTGAA TCTAGATTCG ATTGTGATGA AGACAGTTGA ACTACCGACT GCTGTGACTT AGATCTAAGC + 1 A T N T T L A L D V V N L I S M A Styl Ncol 1151 CTACTAACAC TACTTTGGCT TTGGATGTTG TTAACTTGAT CTCCATGGCT GATGATTGTG ATGAAACCGA AACCTACAAC AATTGAACTA GAGGTACCGA +1 K R E E A E P K F V N Q H L C G S 1201 AAGAGAGAAG AAGCTGAACC AAAGTTCGTT AACCAACACT TGTGCGGTTC TTCTCTCTTC TTCGACTTGG TTTCAAGCAA TTGGTTGTGA ACACGCCAAG + 1 H L V E A L Y L V C G E R G F F Hindlll 1251 CCACTTGGTT GAAGCTTTGT ACTTGGTTTG CGGTGAAAGA GGTTTCTTCT GGTGAACCAA CTTCGAAACA TGAACCAAAC GCCACTTTCT CCAAAGAAGA +1 Y T P K S D D A K G I V E Q C C T 1301 ACACTCCTAA GTCTGACGAT GCTAAGGGTA TTGTCGAGCA ATGCTGTACC TGTGAGGATT CAGACTGCTA CGATTCCCAT AACAGCTCGT TACGACATGG +1 S I C S L Y Q L E N Y C N * 1351 TCCATCTGCT CCTTGTACCA ATTGGAAAAC TACTGCAACT AGACGCAGCC AGGTAGACGA GGAACATGGT TAACCTTTTG ATGACGTTGA TCTGCGTCGG Xbal 1401 CGCAGGCTCT AGAAACTAAG ATTAATATAA TTATATAAAA ATATTATCTT GCGTCCGAGA TCTTTGATTC TAATTATATT AATATATTTT TATAATAGAA SEQ ID NO 4 EcoRI 901 TTCTTGCTTA AATCTATAAC TACAAAAAAC ACATACAGGA ATTCCATTCA AAGAACGAAT TTAGATATTG ATGTTTTTTG TGTATGTCCT TAAGGTAAGT 951 AGAATAGTTC AAACAAGAAG ATTACAAACT ATCAATTTCA TACACAATAT TCTTATCAAG TTTGTTCTTC TAATGTTTGA TAGTTAAAGT ATGTGTTATA +1 M K L K T V R S A V L S Bglll 1001 AAACGATTAA AAGAATGAAA CTGAAAACTG TAAGATCTGC GGTCCTTTCG TTTGCTAATT TTCTTACTTT GACTTTTGAC ATTCTAGACG CCAGGAAAGC + 1 S L F A S Q V L G Q P I D D T E S Styl 1051 TCACTCTTTG CATCTCAGGT CCTTGGCCAA CCAATTGACG ACACTGAATC AGTGAGAAAC GTAGAGTCCA GGAACCGGTT GGTTAACTGC TGTGACTTAG +1 N T T S V N L M A D D T E S R F Xbal 1101 TAACACTACT TCTGTCAACT TGATGGCTGA CGACACTGAA TCTAGATTCG ATTGTGATGA AGACAGTTGA ACTACCGACT GCTGTGACTT AGATCTAAGC + 1 A T N T T L A L D V V N L I S M A Styl Ncol 1151 CTACTAACAC TACTTTGGCT TTGGATGTTG TTAACTTGAT CTCCATGGCT GATGATTGTG ATGAAACCGA AACCTACAAC AATTGAACTA GAGGTACCGA +1 K R E E A E P K F V N Q H L C G S 1201 AAGAGAGAAG AAGCTGAACC AAAGTTCGTT AACCAACACT TGTGCGGTTC TTCTCTCTTC TTCGACTTGG TTTCAAGCAA TTGGTTGTGA ACACGCCAAG + 1 H L V E A L Y L V C G E R G F F Hindlll 1251 CCACTTGGTT GAAGCTTTGT ACTTGGTTTG TGGTGAAAGA GGTTTCTTCT GGTGAACCAA CTTCGAAACA TGAACCAAAC ACCACTTTCT CCAAAGAAGA +1 Y T D K A A K G I V E Q C C T S I 1301 ACACTGACAA GGCTGCTAAG GGTATTGTTG AACAATGTTG TACCTCTATC TGTGACTGTT CCGACGATTC CCATAACAAC TTGTTACAAC ATGGAGATAG + 1 C S L Y Q L E N Y C N * 1351 TGTTCTTTGT ACCAATTGGA AAACTACTGT AACTAGACGC AGCCCGCAGG ACAAGAAACA TGGTTAACCT TTTGATGACA TTGATCTGCG TCGGGCGTCC Xbal 1401 CTCTAGACTA AGATTAATAT AATTATATAA AAATATTATC TTCTTTTCTT GAGATCTGAT TCTAATTATA TTAATATATT TTTATAATAG AAGAAAAGAA Xbal 1401 AGGCTCTAGA AACTAAGATT AATATAATTA TATAAAAATA TTATCTTCTT TCCGAGATCT TTGATTCTAA TTATATTAAT ATATTTTTAT AATAGAAGAA SEQ I D NO 5 , LA19 leader INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 43 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67: Gin Pro He Asp Asp Thr Glu Ser Asn Thr Thr Ser al Asn Leu Met 1 5 10 15 Ala Asp Asp Thr Glu Ser Arg Phe Ala Thr Asn Thr Thr Leu Ala Leu 20 25 30 Asp Val Val Asn Leu Me Ser Met Ala Lys Arg 35 40

Claims: CLAIMS 1 A DNA construct encoding a polypeptide and having the followmg structure signal peptide-ieader peptide-EEAEPK-heterologous protein 2 A DNA construct according to the preceding claim, wherein the signal peptide is the yeast aspartic protease 3 (YAP3) signal peptide or a functional analogue thereof 3 A DNA construct according to any one of the preceding claims, wherein the leader peptide is a synthetic leader peptide, preferably the LA19 leader peptide of SEQ ID NO 5 herein 4 A DNA construct according to any one of the preceding claims, wherein the heterologous protein is selected from the group consisting of aprotinin, tissue factor pathway inhibitor or other protease inhibitors, and insulin or insulin precursors, insulin analogues, insulin-like growth factor I or II, human or bovine growth hormone, iπterleuk , tissue plasminogen activator, transforming growth factor a or b, glucagon, glucagon-like peptide 1 (GLP-1), glucagon-like peptide 2 (GLP-2), GRPP, Factor Vll, Factor VIII, Factor XIII, platelet-derived growth factor, enzymes, such as lipases, or a functional analogue of any one of these proteins 5 A DNA construct according to any one of claims 1 , 2, or 3, wherein the heterologous protein is selected from the group consisting of aprotinin, tissue factor pathway inhibitor or other protease inhibitors, insulin-like growth factor I or II, human or bovine growth hormone, interleukm, tissue plasminogen activator, glucagon, glucagon-like peptιde-1 , Factor Vll, Factor VIII, Factor XIII, plateletdeπved growth factor, enzymes, insulin or an insulin precursor, and a functional analogue of any of these proteins A DNA construct according to claim 4 or 5, wherein the heterologous protein is insulin or an insulin precursor, preferably selected from the group consisting of MM , B(1-29)-A(1-21 ), MI3, B(1-29)-Ala-Ala-Lys-A(1-21 ), X14, B(1-27-Asp-Lys)- Ala-Ala-Lys-A(1-21 ), B(1-29)-Ala-Ala-Arg-A(1-21 ), MI5, B(1 -29)-Ser-Asp-Asp-Ala- Lys-A(1 -21 ), and B(1 -29)-Ser-Asp-Asp-Ala-Arg-A(1 -21 ) 7 A DNA construct according to claim 4 or 5, wherein the heterologous protein is insulin or an insulin precursor, preferably the insulin precursor MI3, or a functional analogue thereof 8 The DNA construct described in SEQ ID NO 1 , 2, 3, or 4 herein 9 A recombinant expression vector which is capable of replicating in a eucaryotic cell and which carries a DNA construct according to any one of the preceding claims 10 A recombinant expression vector which is capable of replicating in yeast and which carries a DNA construct according to any one of claims 1 through 8 11 A yeast cell which is capable of expressing a heterologous protein and which is transformed with a vector according to claim 10 12 A yeast cell according to claim 11 selected from the species Saccharomyces cerevisiae, Saccharomyces kluyveπ, Schizosaccharomyces pombe, Saccharomyces uvarum, Kluyveromyces lactis, Hansenula polymorpha Pichia pastoris, Pichia methanolica Pichia kluyveπ, Yarrowia lipolytica, Candida sp , Candida utilis, Candida cacaoi, Geotnchum sp , and Geotnchum fermentans, preferably Saccharomyces cerevisiae 13 A process for producing a heterologous protein, comprising cultivating a yeast cell according to claim 11 or 12 in a suitable medium to obtain expression and secretion of the heterologous protein, after which the protein is isolated from the culture medium and/or the cells 14 A process for producing a heterologous protein, comprising cultivation a yeast cell according to claim 11 or 12 in a suitable medium to obtain expression and secretion of the heterologous protein, after which the protein is isolated from the culture medium 15 A process according to claim 13 or 14, wherein the am o acid sequence EEAEPK is removed from the recovered heterologous protein by a process involving treatment with a proteolytic enzyme which is specific for a basic ammo 16 A process according to claim 15, wherein the proteolytic enzyme is selected from the group consisting of trypsin and Achromobacter lyticus protease I

Inventors: BALSCHMIDT PER, KJELDSEN THOMAS BOERGLUM, PETTERSSON ANNETTE FROST, BALSCHMIDT, PER, KJELDSEN, THOMAS, BOERGLUM, PETTERSSON, ANNETTE, FROST
Applicants: BALSCHMIDT PER, KJELDSEN THOMAS BOERGLUM, NOVONORDISK AS, PETTERSSON ANNETTE FROST, BALSCHMIDT, PER, KJELDSEN, THOMAS, BOERGLUM, NOVO NORDISK A/S, PETTERSSON, ANNETTE, FROST
Application year: 1996
Main classification: C12N15
IPC codes: C07K14, C12N1, C12N15