INSULIN DERIVATIVES

Abstract: Insulin derivatives in which a lipophilic group having from 12 to 40 carbon atoms is attached to the alpha -amino group of the N-terminal amino acid in the B-chain or to the carboxy group of the C-terminal amino acid in the B-chain have a protracted profile of action.

Description: INSULIN DERIVATIVES FIELD OF THE INVENTION The present invention relates to novel human insulin derivatives which are soluble and have a protracted profile of action, to a method of providing such derivatives, to pharmaceutical compositions containing them, and to the use of such insulin derivatives in the treatment of diabetes. BACKGROUND OF THE INVENTION Many diabetic patients are treated with multiple daily insulin injections in a regimen comprising one or two daily injections of a protracted insulin to cover the basal requirement supplemented by bolus injections of a rapid acting insulin to cover the meal- related requirements. Protracted insulin compositions are well known in the art. Thus, one main type of protracted insulin compositions comprises injectable aqueous suspensions of insulin crystals or amorphous insulin. In these compositions, the insulin compounds utilized typically are protamine insulin, zinc insulin or protamine zinc insulin. Certain drawbacks are associated with the use of insulin suspensions. Thus, in order to secure an accurate dosing, the insulin particles must be suspended homogeneously by gentle shaking before a defined volume of the suspension is withdrawn from a vial or expelled from a cartridge. Also, for the storage of insulin suspensions, the temperature must be kept within more narrow limits than for insulin solutions in order to avoid lump forma-ion or coagulation. While it was earlier believed that protamines were non- im unogenic, it has now turned out that protamines can be immunogenic in man and that their use for medical purposes may lead to formation of antibodies (Samuel et al . , Studies on tne immunogenicity of protamines in humans and experimental animals by means of a micro-complement fixation test, Clm Exp. Immunol. 3_3, pp. 252-260 (1978)) . Also, evidence has been found that the protamme-insulm complex is itself lmmunogenic (Kurtz et al . , Circulating IgG antibody to protamine in patients treated with protamine- msulins. Diabetologica 2jj, pp 322-324 (1983)) Therefore, with some patients the use of protracted insulin compositions containing protamines must be avoided Another type of protracted insulin compositions are solutions having a pH value below physiological pH from which the insulin will precipitate because of the rise in the pH value when the solution is injected. A drawback is that the solid particles of the insulin act as a local irritant causing inflammation of the tissue at the site of injection. WO 91/12817 (Novo Nordisk A/S) discloses protracted, soluble insulin compositions comprising insulin complexes of cobalt (III) The protraction of these complexes is only intermediate and the bioavailability is reduced Human insulin has three primary ammo groups the N-termmal group cf the A-chain and of the B-chain and the e -amino group of Lys B2 ° Several insulin derivatives which are substituted in one or more of these groups are known in the prior art Thus, US Patent No. 3,528,960 (Eli Lilly) relates to N-carboxyaroyl insulins m which one, two or three primary amino groups of the insulin molecule has a carboxyaroyl group No specifically N s32" - substituted insulins are disclosed According to GB Patent No. 1.492 997 (Nat Res. Dev Corp ) , it has been found that insulin with a caroa yl substitution at N iB2Q has an improved profile of hypoglycaemic effect JP laid-open patent application No. 1-254699 (Kodama Co., Ltd.) discloses insulin wherein a fatty acid is bound to the amino group of Phe B1 or to the e-amino group of Lys B29 or to both of these. The stated purpose of the derivatisation is to obtain a s pharmacologically acceptable, stable insulin preparation. Insulins, which in the B30 position has an amino acid having at least five carbon atoms which cannot necessarily be coded for by a triplet of nucleotides, are described in JP laid-open patent application No. 57-067548 (Shionogi) . The insulin o analogues are claimed to be useful in the treatment of diabetes mellitus, particularly in patients who are insulin resistant due to generation of bovine or swine insulin antibodies. US 5,359,030 (Ekwuribe, Protein Delivery, Inc.) describes conjugation-stabilized polypeptide compositions for oral or s parenteral administration comprising a polypeptide covalently coupled with a polymer including a linear polyalkylene moiety and a lipophilic moiety, said moieties being arranged so relative to each other that the polypeptide has an enhanced in vivo resistance to enzymatic degradation. 0 EP 511600 A2 relates i.a. to protein derivatives of the formula [protein] [Z] n wherein [protein] represents a protein having n amino residues each derivable from an amino group by removal of one of its hydrogen atoms, in stead of amino groups, [Z] is a residue represented by the formula -CO-W-COOH wherein W is a 5 divalent long chain hydrocarbon group which may also contain certain hetero atoms and n represents an average of the number of amide bonds between [Z] and [protein] . It is mentioned that the protein derivatives of the invention have an extremely prolonged serum half-life as compared with the proteins from o which they are derived and that they exhibit no antigenicity. It is also mentioned, that insulin is one of the proteins from which derivatives according to the invention can be made, but no specific insulin derivatives are disclosed in EP 511600 nor is there any indication of a preferred [Z] or (a) preferred position (s) in which [Z] should be introduced in order to obtain useful insulin derivatives. In the present specification, whenever the term insulin is used in a plural or a generic sense it is intended to encompass both 5 naturally occurring insulins and insulin analogues and derivatives thereof. By "insulin derivative" as used herein is meant a polypeptide having a molecular structure similar to that of human insulin including the disulphide bridges between Cys A7 and Cys B7 and between Cys* 20 and Cys Bl9 and an internal 0 disulphide bridge between Cys A6 and Cyε A11 , and whicn have insulin activity. However, there still is a need for protracted injectable insulin compositions which are solutions and contain insulins which stay in solution after injection and possess minimal s inflammatory and immunogenic properties. One object of the present invention is to provide human insulin derivatives, with a protracted profile of action, which are soluble at physiological pH values. Another object of the present invention is to provide a o pharmaceutical composition comprising the human insulin derivatives according to the invention. It is a further object of the invention to provide a method of making the human insulin derivatives of the invention. SUMMARY OF THE INVENTION 5 Surprisingly, it has turned out that certain insulin derivatives, wherein either the amino group of the N-terminal amino acid of the B-chain has a lipophilic substituent comprising from 12 to 40 carbon atoms attached, or wherein the carboxylic acid group of the C-terminal amino acid of the B- 0 chain has a lipophilic substituent comprising from 12 to 40 carbon atoms attached, have a protracted profile of action and are soluble at physiological pH values. Accordingly, in its broadest aspect, the present invention relates to an insulin derivative having the following sequence: A- Chain S S I 7 i Gly - lie -Val -Glu -Gin -Cys -Cys -Thr- Ser- lie - Cys -Ser- 1 2 3 4 5 6 I 8 9 10 11 12 S I B - Chain S I Xaa -Xaa - Xaa - Gin -His -Leu -Cys -Gly- Ser -His -Leu- Val - 1 2 3 4 5 6 7 8 9 10 11 12 A-Chain (contd. ) 20 Leu-Tyr- -Gln-Leu-Glu-Asn-Tyr-Cys-Xaa ( SEQ ID NO : l ) 13 14 15 16 17 18 19 1 21 B - Chain ( contd . ) Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe- 13 14 15 16 17 18 19 20 21 22 23 24 B-Chain (contd.) Phe-Xaa-Xaa-Xaa-Xaa-Xaa (SEQ ID NO: 2) 25 26 27 28 29 30 wherein Xaa at position A21 is any codable amino acid except Lys, Arg and Cys; Xaa at positions Bl, B2, B3 , B26, B27, B28 and B29 are, independent of each other, any codable amino acid except Cys or deleted; Xaa at position B30 is any codable amino acid except Cys, a dipeptide comprising no Cys or Arg, a tripeptide comprising no Cys or Arg, a tetrapeptide comprising no Cys or Arg or deleted; and either the amino group of the N-terminal amino acid of the B-chain has a lipophilic group, W, attached to it which group has from 12 to 40 carbon atoms and optionally contains a group which can be negatively charged or the carboxyl group of the C-terminal amino acid of the B-chain has a lipophilic group, Z, attached to it which group has from 12 to 40 carbon atoms and optionally contains a group which can be s negatively charged with the proviso that if one or more of the amino acids at position Bl, B2 and B3 is (are) deleted then the number of the N-terminal amino acid is found by counting down from Cys B7 which is always assigned the number 7 and that (a) when B1-B2-B3 is Phe-Val-Asn and A21 is Asn and B26-B27- o B28-B29-B30 is Tyr-Thr-Pro-Lys-Thr or Tyr-Thr-Pro-Lys-Ala, then said W or Z always contains a group which can be negatively charged; and (b) when B29 and B30 are deleted and a group Z as defined above is present at the C-terminal amino acid of the B-chain and s neither Bl, B2 nor B3 is deleted then B1-B2 is different from Phe-Val or B26-B27-B28 is different from Tyr-Thr-Pro or both B1-B2 and B26-B27-B28 are different from said sequences; and (c) when B29 and B30 are deleted and a group Z as defined above is present at the C-terminal amino acid of the B-chain and one 0 of Bl, B2 or B3 is deleted then the N-terminal amino acid of the B-chain is different from Val or the sequence B26-B27-B28 is different from Tyr-Thr-Pro or both the N-terminal amino acid of the B-chain and the sequence B26-B27-B28 are different from Val and Tyr-Thr-Pro respectively. 5 In a preferred embodiment, the present invention relates to an insulin derivative having the following sequence: A-Chain S S I 7 I Gly-lie-Val-Glu-Gin-Cys-Cys-Thr-Ser-lie-Cys-Ser- 1 2 3 4 5 6 I 8 9 10 11 12 S B-Chain S 5 Xaa - Xaa - Xaa - Gin - His - Leu - Cys -Gly - Ser -Hi s - Leu -Val • 1 2 3 4 5 6 7 8 9 10 11 12 A-Chain (contd.) 20 Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Xaa (SEQ ID NO:li 13 14 15 16 17 18 19 | 21 S B-Chain (contd.) Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe- 10 13 14 15 16 17 18 19 20 21 22 23 24 B-Chain (contd.) Phe-Xaa-Xaa-Xaa-Xaa-Xaa (SEQ ID NO:2) 25 26 27 28 29 30 wherein is Xaa at position A21 is any codable amino acid except Lys, Arg and Cys; Xaa at positions Bl, B2 , B3 , B26, B27, B28, B29 and B30 are, independent of each other, any codable amino acid except Cys or deleted; and either the amino group of the N- 20 terminal amino acid of the B-chain has a lipophilic group, W, attached to it which group has from 12 to 40 carbon atoms and optionally contains a group which can be negatively charged or the carboxyl group of the C-terminal amino acid of the B-chain has a lipophilic group, Z, attached to it which group has from 25 12 to 40 carbon atoms and optionally contains a group which can be negatively charged with the proviso that if one or more of the amino acids at position Bl, B2 and B3 is (are) deleted then the number of the N-terminal amino acid is found by counting down from Cys B7 which is always assigned the number 7 and that so (a) when B1-B2-B3 is Phe-Val-Asn and A21 is Asn and B26-B27- B28-B29-B30 is Tyr-Thr-Pro-Lys-Thr or Tyr-Thr-Pro-Lys-Ala, then said W or Z always contains a group which can be negatively charged; and (b) when B29 and B30 are deleted and a group Z as defined above 35 is present at the C-terminal amino acid of the B-chain and neither Bl, B2 nor B3 is deleted then B1-B2 is different from Phe-Val or B26-B27-B28 is different from Tyr-Thr-Pro or both B1-B2 and B26-B27-B28 are different from said sequences; and (c) when B29 and B30 are deleted and a group Z as defined above is present at the C-terminal amino acid of the B-chain and one of Bl, B2 or B3 is deleted then the N-terminal amino acid of the B-chain is different from Val or the sequence B26-B27-B28 is different from Tyr-Thr-Pro or both the N-terminal amino acid of the B-chain and the sequence B26-B27-B28 are different from Val and Tyr-Thr-Pro respectively. When a lipophilic group, W, is attached to the α-amino cjroup of the N-terminal ammo acid of the B-cham, then the bond between the α-amino group and W is preferably an amide bond m which the N-terminal amino group of the B-chain constitutes the amme moiety and a group contained m W constitutes the carboxyl moiety. When a lipophilic group, Z, is attached to the carboxyl group of the C-terminal amino acid of the B-cham, then the bond between the carboxyl group and Z is preferably an amide bond in which the C-terminal carboxyl group constitutes the carboxyl moiety and an ammo group contained in Z constitutes the amme moiety. In another preferred embodiment, the invention relates to an insulin derivative as described above wherein a lipophilic group, W, is attached to the α-ammo group of the N-termmal ammo acid of the B-cham. In another preferred embodiment, the invention relates to a insulin derivative as described above wherein a lipophilic group, Z, is attached to the carboxyl group of the C- erminal ammo acid of the B-chain. In another preferred embodiment, the invention relates to an insulin derivative wherein the ammo acid at position A21 is selected from the group comprising Ala, Asn, Gin, Glu, Gly and Ser. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position Bl is Phe. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position Bl is deleted. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B2 is selected from the group comprising Ala and Val . In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B3 is selected from the group comprising Asn, Gin, Glu and Thr. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B26 is Tyr. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B27 is Thr. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B28 is Pro. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B29 is Lys or Thr. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B28 is Lys and the amino acid at position B29 is Pro. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B30 is Thr or e-acylated Lys. In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO: 2 designates the dipeptide Thr-Lys. In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO: 2 designates the dipeptide Gly-Lys. In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO:2 designates the tripeptide Glu-Ser-Lys. In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO:2 designates the tripeptide Thr-Gly-Lys. In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO: 2 designates the tetrapeptide Thr-Gly-Gly-Lys . In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO: 2 designates the tetrapeptide Thr-Glu-Gly-Lys . In another preferred embodiment, the invention relates to an insulin derivative wherein Xaa at position 30 in SEQ ID NO: 2 designates the tetrapeptide Gly-Asp-Thr-Lys . In another preferred embodiment, the invention relates to an insulin derivative wherein the C-terminal amino acid of the B- chain is e-acylated Lys and the amino acid next to the C- terminal amino acid is Gly. In another preferred embodiment, the invention relates to an insulin derivative wherein the parent insulin is a des(B30) insulin. In another preferred embodiment, the invention relates to an 5 insulin derivative wherein the parent insulin is des(B30) human insulin. In another preferred embodiment, the invention relates to an insulin derivative wherein the parent insulin is a des(B28-B30) insulin. o In another preferred embodiment, the invention relates to an insulin derivative wherein the parent insulin is a des(B27-B30) insulin. In another preferred embodiment, the invention relates to an insulin derivative wherein the parent insulin is a des(B26-B30) s insulin. In another preferred embodiment, the invention relates to an insulin derivative wherein the amino acid at position B28 is Pro and the amino acid at position B29 is Thr. In another preferred embodiment, the invention relates to an o insulin derivative which has a group, W, as mentioned above, attached to the N-terminal α-amino group of its B-chain, W being a group of the general formula CH 3 (CH 2 ) n CH (COOH) NH- CO(CH 2 ) 2 CO- wherein n is an integer from 9 to 15. In another preferred embodiment, the invention relates to an 5 insulin derivative which has a group, W, as mentioned above, attached to the N-terminal α-amino group of its B-chain, W being a group of the general formula CH 3 (CH 2 ),CO- NHCH(COOH) (CH 2 ) 2 CO- wherein r is an integer from 9 to 15. In another preferred embodiment, the invention relates to an 0 insulin derivative which has a group, W, as mentioned above, attached to the N-terminal α-amino group of its B-chain, W being a group of the general formula CH 3 (CH 2 ) s CO- NHCH ( (CH 2 ) 2 COOH) CO- wherein s is an integer from 9 to 15. In another preferred embodiment, the invention relates to an 5 insulin derivative which has a group, Z, as mentioned above, attached to the C-terminal amino acid of its B-chain, wherein Z is a group of the general formula -NHCH(COOH) (CH 2 ) .NH- CO(CH 2 ) m CH 3 wherein m is an integer from 8 to 18, that is, Z is a N e -acylated lysine residue. o In another preferred embodiment, the invention relates to an insulin derivative which has a group, Z, as mentioned above, attached to the C-terminal amino acid of its B-chain, wherein Z is a group of the general formula -NHCH (COOH) (CH 2 ) 4 NH- COCH( (CH 2 ) 2 COOH)NH-CO(CH 2 ) p CH 3 wherein p is an integer from 10 to s 16. In another preferred embodiment, the invention relates to an insulin derivative which has a group, Z, as mentioned above, attached to the C-terminal amino acid of its B-chain, wherein Z is a group of the general formula -NHCH (COOH) (CH 2 ) 4 NH- o CO (CH 2 ) 2 CH (COOH)NH-CO(CH 2 ) q CH 3 wherein q is an integer from 10 to 16. In another preferred embodiment, the invention relates to an insulin derivative which has a group, Z, as mentioned above, which comprises a partly or completely hydrogenated 5 cyclopentanophenanthrene skeleton. In another preferred embodiment, the invention relates to an insulin derivative which has a group, Z, as mentioned above, which is an acylated amino acid, in particular acylated lysine. In another preferred embodiment, the invention relates to Thr B29 0 human insulin with a group Z as described above attached to the C-terminal amino acid of its B-chain. In another preferred embodiment, the invention relates to des(B28-B30) human insulin with a group Z as described above attached to the C-terminal amino acid of its B-chain. In another preferred embodiment, the invention relates to s des(B27-B30) human insulin with a group Z as described above attached to the C-terminal amino acid of its B-chain. In another preferred embodiment, the invention relates to des(B26-B30) human insulin with a group Z as described above attached to the C-terminal amino acid of its B-chain. o In another preferred embodiment, the invention relates to the use of an insulin derivative according to the invention for the preparation of a medicament for treating diabetes. In another preferred embodiment, the invention relates to a pharmaceutical composition for the treatment of diabetes in a s patient in need of such a treatment comprising a therapeutically effective amount of an insulin derivative according to the invention together with a pharmaceutically acceptable carrier. In another preferred embodiment, the invention relates to a 0 pharmaceutical composition for the treatment of diabetes in a patient in need of such a treatment comprising a therapeutically effective amount of an insulin derivative according to the invention, in mixture with an insulin or an insulin analogue which has a rapid onset of action, together 5 with a pharmaceutically acceptable carrier. In another preferred embodiment, the invention relates to a pharmaceutical composition comprising an insulin derivative according to the invention which is soluble at physiological pH values . o In another preferred embodiment, the invention relates to a pharmaceutical composition comprising an insulin derivative according to the invention which is soluble at pH values in the interval from about 6.5 to about 8.5. In another preferred embodiment, the invention relates to a protracted pharmaceutical composition comprising an insulin derivative according to the invention. In another preferred embodiment, the invention relates to a pharmaceutical composition which is a solution containing from about 120 nmol/ml to about 1200 nmol/ml, preferably about 600 nmol/ml of an insulin derivative according to the invention. In another preferred embodiment, the invention relates to a method of treating diabetes in a patient in need of such a treatment comprising administering to the patient a therapeutically effective amount of an insulin derivative according to this invention together with a pharmaceutically acceptable carrier. In another preferred embodiment, the invention relates to a method of treating diabetes in a patient in need of such a treatment comprising administering to the patient a therapeutically effective amount of an insulin derivative according to this invention, in mixture with an insulin or an insulin analogue which has a rapid onset of action, together with a pharmaceutically acceptable carrier. Examples of preferred insulin derivatives according to the present invention are the following: (N' B3: -tetradecanoyl) Thr B29 , Lys B3c human insulin, (N e325 -tetradecanoyl) Lys 329 des(B29,B30) human insulin, (N eB" -tetradecanoyl) Lys B ? des(B28-B30) human insulin and (N E326 -tetradecanoyl) Lys 326 des(B27-B30) human insulin. (N' B32 -tetradecanoyl) Glu B3 °, Ser B31 , Lys B32 human insulin. (N £Ξ2s -acetyl,N e332 -tetradecanoyl) Glu 330 , Ser B3i , Lys B32 human insulin. BRIEF DESCRIPTION OF THE DRAWINGS The present invention is further illustrated with reference to the appended drawing wherein Fig. 1 shows the construction of the plasmids pKV153, pKV159, s pJB173, pJB174 and pJB175; Fig. 2a which is continued in Fig. 2b shows the sequence of pMT742, position 907 to 1500, and the oligonucleotides #94, #593, #2371 and #3075 used for PCR1A, PCR1B and PCR1C of Example 1. The 138 amino acid sequence corresponding to the MF o alpha prepro-leader (amino acids Nos. 1-85) and an insulin precursor which has the amino acid sequence B(l- 29)AlaAlaLysA(l-21) wherein A(l-21) is the A chain of human insulin and B(l-29) is the B chain of human insulin in which Thr(B30) is missing, is shown below the coding sequence (amino s acids Nos. 86-138) . DETAILED DESCRIPTION OF THE INVENTION Terminology The three letter codes and one letter codes for the amino acid residues used herein are those stated in J. Biol. Chem. 243 , p. o 3558 (1968) . In the DNA sequences, A is adenine, C is cytosine, G is guanine, and T is thymine . The following acronyms are used: DMSO for dimethyl sulphoxide, 5 DMF for dimethylformamide, Boc for tert-butoxycarbonyl, NMP for l-methyl-2-pyrrolidone, TFA for trifluoroacetic acid, X-OSu for an N-hydroxysuccinimid ester, 0 X for an acyl group, RP-HPLC for reversed phase high performance liquid chromatography. Preparation of lipophilic insulin derivatives The insulin derivatives according to the present invention can s be prepared i.a. as described in the following: 1. Insulin derivatives featuring in position B30 an amino acid residue which can be coded for by the genetic code, e.g. threonine (human insulin) or alanine (porcine insulin) . 1.1 Insulins modified by attachment of a lipophilic group, W, o to the N-terminal amino group, starting from human insulin. Human insulin is treated with a Boc-reagent (e.g. di- ert-butyl dicarbonate) to form (Al,B29) -diBoc human insulin, i.e. , human insulin in which the N-terminal end of the A-chain and the e- amino group of Lys B29 are protected by a Boc-group. After an s optional purification, e.g. by HPLC, an acyl group is introduced in the α-amino group of Phe B1 by allowing the product to react with a N-hydroxysuccinimide ester of the formula W-OSu wherein W is an acyl group as defined in the above to be introduced at the N-terminal α-amino group of the B-chain. In o the final step, TFA is used to remove the Boc-groups and the product, N αB1 -W human insulin, is isolated. 2. Insulin derivatives with no amino acid residue in position B30, i.e. des(B30) insulins. 2.1 Starting from human insulin or porcine insulin. 5 On treatment with carboxypeptidase A in ammonium buffer, human insulin and porcine insulin both yield des(B30) insulin. After an optional purification, the des(B30) insulin is treated with a Boc-reagent (e.g. di- tert-butyl dicarbonate) to form (A1,B29) -diBoc des(B30) insulin. After an optional purification, e.g. by HPLC, an acyl group is introduced in the α-amino group of the amino acid in position Bl by allowing the product to react with a N-hydroxysuccinimide ester of the s formula W-OSu wherein W is the acyl group to be introduced. In the final step, TFA is used to remove the Boc-groups and the product, (N αB1 -W) des(B30) insulin, is isolated. 2.2 Starting from a single chain human insulin precursor. A single chain human insulin precursor, which is extended in o position Bl with an extension (Ext) which is connected to Bl via an arginine residue and which has a bridge from a C- terminal lysine in position B26, B27, B28 or B30 to Al can be a used as starting material. Preferably, the bridge is a peptide of the formula Y n -Arg, where Y is a codable amino acid s except cysteine, lysine and arginine, and n is zero or an integer between 1 and 35. When n>l, the Y's may designate different amino acids. Preferred examples of the bridge from Lys in position B26, B27, B28 or B30 to Al are: AlaAlaArg, SerArg, SerAspAspAlaArg and Arg (European Patent No. 163529) . o Treatment of such a precursor of the general formula Ext-Arg- B (1-Q) -Y n -Arg-A(l-21) , wherein Q is 26, 27, 28 or 30, with a lysyl endopeptidase, e.g. Achromobacter lyti cus protease, yields Ext-Arg-B (1-Q) Y n -Arg-A(1-21) insulin. Acylation of this intermediate with a N-hydroxysuccinimide ester of the general 5 formula X-OSu wherein X is an acyl group, introduces the acyl group X in the e-amino group of Lys B0 , and in the N-terminal amino group of the A-chain and the B-chain to give (N e30 -X) X- Ext-Arg-B '1-Q) X-Y n -Arg-A(1-21) insulin. This intermediate on treatment with trypsin in mixture of water and a suitable 0 organic solvent, e.g. DMF, DMSO or a lower alcohol, gives the desired derivative, Z-human insulin wherein Z is Lys £BQ -X. Pharmaceutical compositions Pharmaceutical compositions containing a human insulin derivative according to the present invention may be administered parenterally to patients m need of such a treatment . Parenteral administration may be performed by subcutaneous, intramuscular or intravenous injection by means of a syringe, optionally a pen-like syringe. Alternatively, parenteral administration can be performed by means of an infusion pump. A further option is a composition which may be a powder or a liquid for the administration of the human msulm derivative m the form of a nasal spray. Pharmaceutical compositions containing a compound of the present invention may be prepared by conventional techniques, e.g. as described m Remington's Pharmaceutical Sciences, 1985 Thus, the injectable human msulm compositions of the invention can be prepared using the conventional techniques of the pharmaceutical industry which involves dissolving and mixing the ingredients as appropriate to give the desired end product . Thus, according to one procedure, the human msulm derivative is dissolved m an amount of water which is somewhat less than the final volume of the composition to be prepared. An lsotonic agent, a preservative and a buffer is added as required and the pH value of the solution is adjusted - if necessary - using an acid, e.g. hydrochloric acid, or a base, e.g aqueous sodium hydroxide as needed. Finally, the volume of the solution is adjusted with water to give the desired concentration of the ingredients . Examples of lsotonic agents are sodium chloride, mannitol and glycerol Examples of preservatives are phenol, m-cresol, methyl p- hydroxybenzoate and benzyl alcohol Examples of suitable buffers are sodium acetate and sodium phosphate . Preferred pharmaceutical compositions of the particular insulins of the present invention are solutions hexameric 5 complexes. Typically the hexameric complexes are stabilized by two or more zinc ions and three or more molecules of a phenolic compound like phenol or meta-cresol or mixtures thereof per hexamer. In a particular embodiment, a composition is provided which o contains two different insulins, one having a protracted profile of action and one having a rapid onset of action, m the form of soluble hexameric complexes. Typically the hexameric complexes are stabilized by two or more zinc ions and three or more molecules of a phenolic compound like phenol or s meta-cresol or mixtures thereof per hexamer. The complexes are mixtures of hexamers of the particular insulins and mixed hexamers in which the ratio between the two different insulins IS from 1:5 to 5:1. A composition for nasal administration of an insulin derivative 0 according to the present invention may, for example, be prepared as described in European Patent No. 272097 (to Novo Nordisk A/S) . The msulm compositions of this invention can be used m the treatment of diabetes. The optimal dose level for any patient 5 will depend on a variety of factors including the efficacy of the specific human msulm derivative employed, the age, body weight, physical activity, and diet of the patient, on a possible combination with other drugs, and on the severity of the case of diabetes. It is recommended that the daily dosage c of the human insulin derivative of this invention be determined for each individual patient by those skilled m the art a similar way as for known insulin compositions. Where expedient, the human insulin derivatives of this invention may be used in mixture with other types of insulin, e.g. human insulin or porcine insulin or insulin analogues with a more rapid onset of action. Examples of such insulin analogues are described e.g. in the European patent applications having the publication Nos. EP 214826 (Novo Nordisk A/S) , EP 375437 (Novo Nordisk A/S) and EP 383472 (Eli Lilly & Co. ) . The present invention is further illustrated by the following examples which, however, are not to be construed as limiting the scope of protection. The features disclosed in the foregoing description and in the following examples may, both separately and in any combination thereof, be material for realizing the invention in diverse forms thereof. EXAMPLES Plasmids and DNA material All expression plasmids are of the cPOT type. Such plasmids are described in EP patent application No. 171 142 and are characterised in containing the Schi zosaccharomyces pombe triose phosphate isomerase gene (POT) for the purpose of plasmid selection and stabilisation. A plasmid containing the POT-gene is available from a deposited E. coli strain (ATCC 39685) . The plasmids furthermore contain the S . cerevisiae triose phosphate isomerase promoter and terminator (P TP: and T TPI ) . They are identical to pMT742 (Egel-Mitani, M et al . , Gene 73 (1988) 113-120) (see Fig. 1) except for the region defined by the EcoRI-Xbal restriction sites encompassing the coding region for MF alpha prepro leader/product. The EcoRI/Xbal fragment of pMT742 itself encodes the Mating Factor (MF) alpha prepro-leader sequence of Saccharomyces cerevi siae followed by the insulin precursor MI3 which has a Ala-Ala-Lys bridge connecting B29 and Al (i.e. B (1-29) -Ala-Ala-Lys-A(1-21) ) (see Fig. 2) Synthetic DNA fragments were synthesised on an automatic DNA synthesizer (Applied Biosystems model 380A) using phosphoramidite chemistry and commercially available reagents (Beaucage, S.L. and Caruthers, M.H., Tetrahedron Letters 22 s (1981) 1859-1869) . All other methods and materials used common state of the art knowledge (see, e.g. Sambrook, J. , Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory Press, New York, 1989) . o Analytical Molecular masses of insulin precursors prepared were obtained by mass spectroscopy (MS) , either by plasma desorption mass spectrometry (PDMS) using Bio-Ion 20 instrument (Bio-Ion Nordic AB, Uppsala, Sweden) or electrospray mass spectrometry (ESMS) s using an API III Biomolecular Mass Analyzer (Perkin Elmer Sciex Instruments, Thornhill, Canada) . The lipophilicity of an insulin derivative relative to human insulin, k' rel , was measured on a LiChrosorb ® RP18 (5μm, 250x4 mm) HPLC column by isocratic elution at 40°C using mixtures of 0 A) 0.1 M sodium phosphate buffer, pH 7.3, containing 10% acetonitrile, and B) 50% acetonitrile in water. The elution was monitored by the absorption of the eluate at 214 nm. Void time, t 0 , was found by injecting 0.1 mM sodium nitrate. Retention time for human insulin, t human , was adjusted to at least 2t 0 by varying the ratio between the A and B solutions. k' rel is defined as > '-derivative '-o' / y '-human- ^-0 ' ■ As a measure of the protraction of the compounds of the invention, the disappearance rate in pigs was studied and T 50 ., was determined. T 50% is the time when 50% of the A14 Tyr( 125 D - labeled analogue has disappeared from the site of injection as measured with an external γ-counter (Ribel, U et al . , The Pig as a Model for Subcutaneous Absorption in Man. In: M. serrano- Rios and P.J. Lefebre (Eds) : Diabetes 1985; Proceedings of the 12th Congress of the International Diabetes Federation, Madrid, Spain, 1985 (Excerpta Medica, Amsterdam, (1986) 891-96) . EXAMPLE 1 Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala-Thr- Arg-B (1-29) -Ser-Asp-Asp-Ala-Arg-A(1-21) insulin precursor from yeast strain yKV153 using the S . cerevisiae MF alpha prepro- leader. The following oligonucleotides were synthesised: #593 5' -CCAAGTACAAAGCTTCAACCAAGTGGGAACCGCACAAGTGTTGGTTAA CGAATCTTGTAGCCTTTGGTTCAGCTTCAGCTTCAGCTTCTTCTCTTTTAT CCAAAGAAACACC-3 ' #94 5' -TAAATCTATAACTACAAAAAACACATA-3 ' #3075 5 ' -TTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTCTAC ACTCCTAAGTCTGACGATGCTAGAGGTATTG-3 ' #2371 5' -TTAATCTTAGTTTCTAGAGCCTGCGGG-3' The following two Polymerase Chain Reactions (PCR) were performed using Gene Amp PCR reagent kit (Perkin Elmer, 761 Main Avewalk, CT, USA) according to the manufacturer's instructions (see Fig. 2) . PCR1A: 0.2 μl of pMT742 plasmid template 4.0 μl of oligonucleotide #593 (100 pmol! .0 μl of oligonucleotide #94 (100 pmol) 10.0 μl of lOx PCR buffer 10.0 μl of 2.5 Mm dNTP 0.5 μl of Taq polymerase enzyme 71.3 μl of water PCRIB 0.2 μl of pMT742 plasmid template 4.0 μl of oligonucleotide #3075 (100 pmol) 4.0 μl of oligonucleotide #2371 (100 pmol) 10.0 μl of lOx PCR buffer o 10.0 μl of 2.5 mM dNTP 0.5 μl of Taq polymerase enzyme 71.3 μl of water In both cases two cycles were performed at 94 °C for 1 min., 45 °C for 1 min. and 72 °C for 1 min. and subsequently followed by s 11 cycles: 94 °C for 1 min., 55 °C for 1 min., 72 °C for 1 min. 20 μl of each PCR mixture was loaded onto a 2% agarose gel and subjected to electrophoresis using standard techniques (Sambrook et al . , Molecular cloning, Cold Spring Harbour 0 Laboratory Press, 1989) . Resulting DNA fragments (452 bp from PCRIA and 170 bp from PCRIB) were cut out of the agarose gel and isolated using the Gene Clean kit (BiolOl Inc., PO BOX 2284, La Jolla, CA, USA) according to the manufacturer's instructions. The purified DNA fragments were dissolved in 100 s μl of water. The following PCR was performed PCR1C: 1.0 μl of DNA fragment from PCRIA 0 1.0 μl of DNA fragment from PCRIB 10.0 μl of 10 x PCR buffer 10.0 μl of 2.5 mM dNTP 24 0.5 μl of Taq polymerase enzyme 69.5 μl of water Two cycles were performed at 94 °C for 1 mm , 45 °C for 1 mm and 72 °C for 1 mm. Subsequently 4 μl of oligonucleotide #94 (100 pmol) and 4.0 μl of oligonucleotide #2371 (100 pmol) was added and 15 cycles were performed at 94 °C for 1 mm., 55 °C for 1 mm. and 72 °C for 1 mm. The PCR mixture was loaded onto a 1% agarose gel and the resulting 594 bp fragment was purified using the Gene Clean kit as described The purified PCR DNA fragment was dissolved in 20 μl of water and restriction endonuclease buffer and cut with the restriction endonucleases £coRI and Xfoal (New England Biolabs, Inc. MA, USA) . The resulting 550 bp EcoRI/Xbal restriction fragment was run on agarose gel and isolated and purified using the Gene clean kit. In two separate restriction endonuclease digestions the plasmid pMT742 was cut with l) restriction endonucleases Apal and Xbal and ii) with restriction endonucleases Apal and EcoRl From these digestions the 8.6 kb Apal /Xbal restriction fragment and the 2 1 kb Apal/EcoRl restriction fragment was isolated The three fragments (i.e. the 550 bp EcoRI/Xjbal restriction fragment from PCR1C and the 8.6 kb Apal /Xbal restriction fragment and 2.1 kb Apal / EcoRl restriction fragment from pMT742) were ligated together using T4 DNA ligase and standard conditions (Sambrook et al . , Molecular cloning, Cold Spring Harbour Laboratory Press, 1989) (see Fig 1) The ligation mixture was transformed into competent E col i cells (Ap r ) followed by selection for ampicillm resistance Plasmids were isolated from the resulting E . coll colonies using standard DNA miniprep technique (Sambrook et al . , Molecular cloning, Cold Spring Harbour Laboratory Press, 1989) and checked with appropriate restriction endonucleases (i.e. EcoRl , Apal and Xbal) . The selected plasmid designated pKV153 was shown by DNA sequencing analysis (using the Sequenase kit from U.S. Biochemical Corp. according to the manufacturer's recommendations) to contain the correct sequence encoding the MFalphaprepro-leader/Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys- Ala-Thr-Arg-B (1-29) -Ser-Asp-Asp-Ala-Arg-A (1-21) insulin precursor. pKV153 was transformed into S. cerevisiae strain MT663 and selected for growth on glucose as described in European patent application having the publication No. 214826. The resulting yeast strain was designated yKV153 and was verified to produce Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys- Ala-Thr-Arg-B (1-29) -Ser-Asp-Asp-Ala-Arg-A ( 1-21) insulin precursor in the culture media by HPLC and mass spectroscopy. EXAMPLE 2 Synthesis of Lys B30 (N € -tetradecanoyl) Thr B29 human insulin 2a. Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-28) -Thr-Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) insulin precursor from yeast strain yKV159 using the S . cerevisiae MF alpha prepro-leader. The following oligonucleotides were synthesised: #3881 5' -TTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTCTAC ACTCCTACCAAGTCTGACGATGCTAGAGGTATTGTCG-3 ' #2371 5' -TTAATCTTAGTTTCTAGAGCCTGCGGG-3' The following Polymerase Chain Reaction (PCR) was performed using Gene Amp PCR reagent kit as described above . PCR2 0.2 μl of pKV153 plasmid template (from Example 1) 4.0 μl of oligonucleotide #3881 (100 pmol) 4.0 μl of oligonucleotide #2371 (100 pmol) s 10.0 μl of lOx PCR buffer 10.0 μl of 2.5 mM dNTP 0.5 μl of Taq polymerase enzyme 71.3 μl of water Two cycles were performed at 94 °C for 1 min., 45 °C for 1 min. o and 72 °C for 1 min. and subsequently followed by 11 cycles at 94 °C for 1 min., 55 °C for 1 min., 72 °C for 1 min. The PCR mixture was loaded onto a 2% agarose gel and the resulting 174 bp fragment was purified using the Gene Clean kit as described. The purified PCR DNA fragment was dissolved in 20 s μl of water and restriction endonuclease buffer and cut with the restriction endonucleases HindiII and Xbal . The resulting 153 bp HindiII/Xbal restriction fragment was run on agarose gel and isolated and purified using the Gene Clean kit. In two separate restriction endonuclease digestions pMT742 was 0 cut with restriction endonucleases Apal and Xbal whereas pKV153 (from Example 1) was cut with restriction endonucleases Apal and EcoRl . From this digestions the 8.6 kb Apal/Xbal restriction fragment from pMT742 and the 2.1 kb Apal / EcoRl restriction fragment from pKV153 was isolated. 5 The three fragments (i.e. the 550 bp EcoRl / Xbal restriction fragment from PCR2 and the 8.6 kb Apal /Xbal restriction fragment from pMT748 and the 2.1 kb Apal / EcoRl restriction fragment from pKV153) were ligated together using T4 DNA ligase as described above (see Fig. 1) . The ligation mixture was 0 transformed into competent E . col i cells (Ap r" ) followed by selection for ampicillin resistance. Plasmids were isolated from the resulting E. col i colonies and checked for appropriate restriction endonucleases (i.e. Hindlll, Apal and Xbal) as described. The selected plasmid designated pKV159 was shown by DNA sequencing analysis to contain the correct sequence encoding the MF alpha prepro-leader/Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro- Lys-Ala-Thr-Arg-B (1-28) -Thr-Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) 5 insulin precursor. pKV159 was transformed into S. cerevisiae strain MT663 and selected for growth on glucose as described. The resulting yeast strain was designated yKV159 and was verified to produce Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys- o Ala-Thr-Arg-B(l-28) -Thr-Lys-Ser-Asp-Asp-Ala-Arg-A(l-21) insulin precursor in the culture media by HPLC and mass spectroscopy. 2b. Isolation of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-28) -Thr-Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) insulin precursor. s The yKV159 strain was fermented for 72 hours and five litres of broth were collected. Yeast cells were removed by centrifugation, the pH value was adjusted to 3.0 using sulphuric acid and the insulin precursor was concentrated on a Pharmacia Strealine ® 50 column packed with 300 ml of o Streamline ® SP ion exchanger. After wash with 25 mM citrate buffer, pH value 3.6, the precursor was eluted by 0.5 M NH 3 and the fraction from 300 to 600 ml was collected. The pH value was adjusted to 2.5 and the precursor was purified by RP-HPLC using 15 μ spherical C18 silica of 100 A pore size and 0.2 M Na 2 S0 4 , 5 0.04 M H 3 P0 4 as buffer, and using a gradient from 23 to 33% acetonitrile. The precursor eluted at about 27-28% acetonitrile. The pool containing the central major peak of the precursor was desalted by gel filtration on Sephadex ® G-50 F m 0.5 M acetic acid, and the precursor isolated by 0 lyophilization. Yield: 486 mg. 2c. Synthesis of Ala-Thr-Arg-B (1-28) -Thr-Lys, Ser-Asp-Asp-Ala- Arg-A(l-21) insulin. The 486 mg of smgle-cham precursor obtained as described above were dissolved m 30 ml of 0.05 M glutamate buffer at pH 9.0, and 3 ml of immobilized A. lyticus protease gel were added (see PCT/DK94/00347, page 45) . After gentle stirring for 5 5 hours at 30°C, the gel was removed by filtration and the double-cham, extended msulm was crystallized by the addition of 10 ml of ethanol, 845 mg of trisodium citrate dihydrate and 78 mg of zmc chloride. After adjustment of the pH value to 6.1 and storage at 4°C overnight the crystals were collected by o centrifugation, washed twice with isopropanol and dried m vacuo . Yield: 450 mg. 2d. Synthesis of N αA 5 ,N a3"3 ,N 6330 -tris (tetradecanoyl) Ala-Thr-Arg- B (1-28) -Thr-Lys, Ser-Asp-Asp-Ala-Arg-A(1-21) insulin. 450 mg of the double-chain, extended msulm, obtained as s described above, were dissolved in a mixture of 3.15 ml of DMSO and 0.69 ml of 2 M diisopropylethylamme m NMP. The solution was cooled to 15°C and 0.69 ml of 0.3 M tetradecanoic acid N- hydroxysuccmimide ester in DMSO/NMP (1:1, v/v) was added. After 2 hours at 15°C, the reaction was stopped by addition of o 112 ml of 0.01 M glycme buffer in ethanol/water (60:40, v/v) and the pH value adjusted to 10.0. The triacylated intermediate was not isolated. 2e. Synthesis of Lys B30 (N f -tetradecanoyl) Thr 329 human msulm To the solution from the previous step was added 5 ml of 5 immobilized trypsm gel (see PCT/DK94/00347, page 46) . After gentle stirring at 15°C for 16 hours, the gel was removed by filtration, tne pH value was adjusted to 9.0 and the solution was applied to a 2.5 x 25 cm column of QAE-Sephadex ® A-25 Isocratic elution was performed at a rate of 17.3 ml/h using a o 0.12 M NH 4 C1 buffer ethanol/water (60:40, v/v) adjusted to pH 9.0 with NK 3 . The title compound emerged from the column after 650 ml, and a pool from 650 to 754 ml was collected Finally, the buffer was changed to 0.01 M NH 4 HC0 3 by gel filtration on Sephadex ® G-50 Fine, and the product isolated in the dry state by lyophilization. Yield: 91 mg. Molecular mass of the title compound, found by MS: 6020 ± 6, theory: 6018. Molecular mass of the B-chain, found by MS: 3642 ± 5, theory: 3640. Molecular mass of the C-terminal fragment of B-chain digested by V8 protease, found by MS: 1326 ± 2, theory: 1326. Relative lipophilicity, k' rel = 113. Disappearance half-life, T 50V , after subcutaneous injection in pigs: 20.3 ± 5.2 hours (n=6) . EXAMPLE 3 Synthesis of Lys 328 (N e -tetradecanoyl) des(B29-B30) human insulin. 3a. Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) insulin precursor from yeast strain yJB173 using the S . cerevisiae MF alpha prepro-leader. The following oligonucleotides were synthesised: #627 5 ' -CACTTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTC TACACTAAGTCTGACGATGCTAG-3 ' #2371 5 ' -TTAATCTTAGTTTCTAGAGCCTGCGGG- ' The DNA encoding Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) was constructed in the same manner as described in Example 2 by substituting oligonucleotide #3881 with oligonucleotide #627. The resulting plasmid was designated pJB173 and the yeast strain expressing Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) was designated yJB173. 3b. Isolation of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) insulin 5 precursor. The yJB173 strain was fermented for 72 hours and 4.8 litres of broth were collected. Yeast cells were removed by centrifugation and the pH value was adjusted to 3.0 using sulphuric acid. The conductivity was 7.8 mS/cm. The insulin o precursor was concentrated using a Pharmacia Streamline ® 50 column packed with 300 ml of Streamline ® SP ion exchanger. After wash with 25 mM citrate buffer, pH value 3.6, the precursor was eluted by 0.5 M NH 3 and the fraction from 300 to 600 ml was collected. Free ammonia was evaporated in vacuo at s room temperature and the pH value of the resulting 280 ml of solution was adjusted to 9.0 with hydrochloric acid. 3c. Synthesis of Ala-Thr-Arg-B (1-27) -Lys, Ser-Asp-Asp-Ala-Arg- A(l-21) insulin. To the 280 ml of solution containing 118 mg of the single-chain o precursor, obtained as described above, were added 3 ml of immobilized A. lyticus protease gel (see PCT/DK94/00347, page 45) . After gentle stirring for 24 hours at 30°C the gel was removed by filtration. The pH value was adjusted to 3.5 and the solution was filtered though a Milipore ® 0.45 μ filter. The 5 double-chain, extended insulin was purified in 2 runs by RP- HPLC using a 2x20 cm column packed with 15 μ spherical C18 silica of 100 A pore size and 0.2 M Na 2 S0 4 , 0.04 M H 3 P0 4 , pH 3.5 as buffer, and using a gradient from 23 to 33% acetonitrile at a rate of 4 ml/min and a column temperature of 40°C. The o double-chain, extended insulin eluted at about 30-31% acetonitrile. The acetonitrile was removed from the combined pools of 70 ml by evaporation in vacuo, and salts were removed by gelfiltration using a 5x47 cm column of Sephadex G-25 in 0.5 M acetic acid. The double-chain, extended insulin was isolated by lyophilization. Yield: 110 mg. 3d. Synthesis of N QA - 5 ,N 0,B"3 ,N eB28 -tris (tetradecanoyl) Ala-Thr-Arg- B(l-27)-Lys, Ser-Asp-Asp-Ala-Arg-A(1-21) insulin. s 110 mg of the double-chain, extended insulin obtained as described above were dissolved in a mixture of 0.84 ml of DMSO and 0.275 ml of 2 M diisopropylethylamine in NMP. The solution was cooled to 15°C and 0.185 ml of 0.3 M tetradecanoic acid N- hydroxysuccinimide ester in DMSO/NMP (1:1, v/v) was added. o After 2 hour at 15°C, the reaction was stopped by addition of 32.5 ml of 0.01 M glycine buffer in ethanol/water (60:40, v/v) and the pH value adjusted to 10.0. The triacylated intermediate was not isolated. 3e. Synthesis of Lys B2B (N e -tetradecanoyl) des(B29-B30) human s insulin. To the resulting solution from the previous step was added 1.5 ml of immobilized trypsin gel (see PCT/DK94/00347, page 46) . After gentle stirring at 15°C for 18 hours, the gel was removed by filtration, the pH value adjusted to 9.0 and the solution o applied to a 1.5 x 21 cm column of QAE-Sephadex " A-25. Isocratic elution was performed at a rate of 10 ml/h using a 0.12 M NH 4 C1 buffer in ethanol/water (60:40, v/v) adjusted to pH 9.0 with NH 3 . The title compound emerged from the column after 250-390 ml, peaking at 330 ml. Finally, the buffer was changed to 0.01 5 M NH 4 HC0 3 by gel filtration using Sephadex ® G-50 Fine, and the product was isolated in the dry state by lyophilization. Yield: 47 mg. Molecular mass of the title compound, found by MS: 5820 ± 2, theory: 5819. 0 Molecular mass of the B-chain, found by MS: 3444 ± 4, theory: 3442. Molecular mass of the C-terminal fragment of B-chain digested by V8 protease, found by MS: 1128 ± 2, theory: 1128. Relative lipophilicity, k' rel = 121. Disappearance half-life, T 50% , after subcutaneous injection in pigs: 19.6 ± 3.6 h (n=4) . EXAMPLE 4 5 Synthesis of Lys B27 (N* -tetradecanoyl) des(B28-B30) human insulin. 4a. Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-26) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) insulin o precursor from yeast strain yJB174 using the S . cerevisiae MF alpha prepro-leader. The following oligonucleotides were synthesised: #628 5- ' CACTTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTC TACAAGTCTGACGATGCTAG-3 ' s #2371 5' -TTAATCTTAGTTTCTAGAGCCTGCGGG-3' The DNA encoding Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-26) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) wasconstructed in the same manner as described in Example 2 by substituting oligonucleotide #3881 with oligonucleotide #628. o The resulting plasmid was designated pJB174 and the yeast strain expressing Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-26) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) was designated yJB174. 4b. Isolation of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- 5 Thr-Arg-B (1-26 ) -Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) insulin precursor. The yJB174 strain was fermented for 72 hours and 3.5 litres of broth were collected. Yeast cells were removed by centrifugation, the pH value was adjusted to 3.0 using sulphuric acid and the solution was diluted with water to 8 litres in order to decrease the salt concentration. The resulting conductivity was 7.9 mS/cm The msulm precursor was 5 concentrated using a Pharmacia Streamline ® 50 column packed with 300 ml of Streamline ® SP ion exchanger. After wash with 25 mM citrate buffer, pH 3.6, the precursor was eluted by 0.5 M NH 3 and the fraction from 300 to 600 ml was collected. Free ammonia was evaporated in vacuo at room temperature and the pH value of o the resulting 280 ml was adjusted to 9.0 with hydrochloric acid. 4c. Synthesis of Ala-Thr-Arg-B (1-26) -Lys, Ser-Asp-Asp-Ala-Arg- A(l-21) insulin To the 280 ml of solution of the single-chain precursor s obtained as described above was added 3 ml of immobilized A lyticus protease gel (see PCT/DK94/00347, page 45) . After gentle stirring for 13 hours at 30°C the gel was removed by filtration. The pH value was adjusted to 2.5 and the solution was filtered though a Milipore ® 0.45 μ filter. The double- o chain, extended msulm was purified in 4 runs by RP-HPLC using a 2x20 cm column packed with 15 μ spherical C18 silica of 100 A pore size and 0.2 M Na 2 S0 4 , 0.04 M H 3 P0 4 , pH 2 5 as buffer, and using a gradient from 24 to 33% acetonitrile. The double-chain, extended msulm eluted at about 30-31% acetonitrile The 5 acetonitrile was removed from the combined pools by evaporation in vacuo, and the salts were removed by gelflltration using a 5x47 cm column of Sephadex G-25 m 0.5 M acetic ac d. The double-cnam, extended msulm was isolated by lyophilization Yield- 69 mg. 04d. Synthesis of N αA 5 ,N oB 3 ,N £B27 -trιs (tetradecanoyl) Ala-Thr-Arg- B(l-26)-Lys, Ser-Asp-Asp-Ala-Arg-A(1-21) msulm 62 mg of the double-chain, extended msulm obtained as describee under 4e was dissolved in a mixture of 0 44 ml of DMSO and 0 15 ml of 2 M dnsopropylethylamme NMP The solution was cooled to 15°C and 0.096 ml of 0.3 M tetradecanoic acid N-hydroxysuccinimide ester in DMSO/NMP (1:1, v/v) was added. After 2 hours at 15°C the reaction was stopped by addition of 17 ml of 0.01 M glycme buffer in ethanol/water s (60:40, v/v) and the pH value adjusted to 10.0. The triacylated intermediate was not isolated. 4e. Synthesis of Lys 327 (N e -tetradecanoyl) des(B28-B30) human msulm. To the solution from the previous step was added 1 ml of o immobilized trypsin gel (see PCT/DK94/00347, page 46) . After gentle stirring at 15°C for 26 hours, the gel was removed by filtration, the pH value adjusted to 9.0 and the solution applied to a 1.5 x 25.5 cm column of QAE-Sephadex ® A-25. Isocratic elution was performed at a rate of 17.3 ml/h using a s 0.12 M NH 4 C1 buffer m ethanol/water (60:40, v/v) adjusted to pH 9.0 with NH 3 . The title compound emerged from the column after 360 ml, and a pool from 272 to 455 ml was collected. Finally, the buffer was changed to 0.01 M NH 4 HC0 3 by gel filtration on Sephadex ® G-50 Fine, and the product isolated m c the dry state by lyophilization. Yield: 38 mg. Molecular mass of the title compound, found by MS: 5720 ± 6, theory: 5718. Molecular mass of the B-cham, found by MS: 3342 ± 4, theory 3340. 5 Molecular mass of the C-termmal fragment of B-cham digested by V8 protease, found by MS: 1027 ± 2, theory: 1027. Relative lipophilicity, k' rel = 151. Disappearance half-life, T 5(n , after subcutaneous injection in 0 EXAMPLE 5 Synthesis of Lys B26 (N ( -tetradecanoyl) des(B27-B30) human msulm. 5a. Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-25) -Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) insulin precursor from yeast strain yJB175 using the S . cerevisiae MF alpha prepro-leader. s The following oligonucleotides were synthesised: #629 5 ' -CACTTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTC AAAGTCTGACGATGCTAG-3' #2371 5' -TTAATCTTAGTTTCTAGAGCCTGCGGG-3 ' The DNA encoding Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- ιo Thr-Arg-B (1-25) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) wasconstructed in the same manner as described in Example 2 by substituting oligonucleotide #3881 with oligonucleotide #629. The resulting plasmid was designated pJB175 and the yeast strain expressing Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- i5 Thr-Arg-B (1-25) -Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) was designated yJB175. 5b. Isolation of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-25) -Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) insulin precursor. 20 The yJB175 strain was fermented for 72 hours and 3.7 litres of broth were collected. Yeast cells were removed by centrifugation, the pH value was adjusted to 3.0 using sulphuric acid and the solution was diluted with water to 8.5 litres in order to decrease the salt concentration. The 25 resulting conductivity was 7.7 mS/cm. The insulin precursor was concentrated using a Pharmacia Strealine ® 50 column packed with 300 ml of Streamline ® SP ion exchanger. After wash with 25 mM citrate buffer, pH 3.6, the precursor was eluted by 0.5 M ammonia and the fraction from 300 to 600 ml was collected. Free 3o ammonia was evaporated in vacuo at room temperature and the pH value of the resulting 270 ml of solution was adjusted to 9.0 with hydrochloric acid. 5c. Synthesis of Ala-Thr-Arg-B (1-25) -Lys, Ser-Asp-Asp-Ala-Arg- A -21) insulin. s To the 270 ml solution of the single-chain precursor obtained as described above was added 3 ml of immobilized A. lyticus protease gel (see PCT/DK94/00347, page 45) . After gentle stirring for 23 hours at 30°C, the gel was removed by filtration. The pH value was adjusted to 2.5 and the solution o was filtered though a Milipore ® 0.45 μ filter. The double- chain, extended insulin was purified in 4 runs by RP-HPLC using a 2x20 cm column packed with 15 μ spherical C18 silica of 100 A pore size and 0.2 M Na 2 S0 4 , 0.04 M H 3 P0 4 , pH 3.5 as buffer, and using a gradient from 24 to 33% acetonitrile. The double-chain, s extended insulin eluted at about 29-31% acetonitrile. The acetonitrile was removed from the combined pools by evaporation in vacuo , and the salts were removed by gelfiltration using a 5x47 cm column of Sephadex ® G-25 in 0.5 M acetic acid. The double-chain, extended insulin was isolated by lyophilization. 0 Yield: 81 mg. 5d. Synthesis of N aA"5 ,N αB"3 ,N eB26 -tris (tetradecanoyl) Ala-Thr-Arg- B (1-25) -Lys, Ser-Asp-Asp-Ala-Arg-A(1-21) sulm. 80 mg of the double-chain, extended insulin was dissolved in a mixture of 0.56 ml of DMSO and 0.19 ml of 2 M 5 diisopropylethylamine in NMP. The solution was cooled to 15°C and 0.124 ml of 0.3 M tetradecanoic acid N-hydroxysuccinimide ester in DMSO/NMP (1:1, v/v) was added. After 2 hour at 15°C the reaction was stopped by addition of 21.8 ml of 0.01 M glycme buffer in ethanol/water (60:40, v/v) and the pH value 0 adjusted to 10.0. The triacylated intermediate was not isolated. 5e. Synthesis of Lys 326 (N' -tetradecanoyl) des (B27-B30) , human msulm. To the solution from the previous step was added 1 ml of immobilized trypsin gel (see PCT/DK94/00347, page 46) . After gentle stirring at 15°C for 23 hours the gel was removed by filtration, the pH value was adjusted to 9.0 and the solution s applied to a 1.5 x 25.5 cm column of QAE-Sephadex ® A-25. Isocratic elution was performed at a rate of 19.3 ml/h using a 0.12 M NH 4 C1 buffer in ethanol/water (60:40, v/v) adjusted to pH 9.0 with ammonia. The title compound emerged from the column after 320 ml, and a fraction from 320 to 535 ml was collected. o Finally, the buffer was changed to 0.01 M NH 4 HC0 3 by gel filtration on Sephadex ® G-50 Fine, and the product isolated in the dry state by lyophilization. Yield: 25 mg. Molecular mass of the title compound found by MS: 5555 ± 6, theory: 5555. s Molecular mass of the B-chain found by MS: 3179 ± 4, theory: 3178. Molecular mass of the C-terminal fragment of B-chain digested by V8 protease found by MS: 864 ± 1, theory: 863.5. Relative lipophilicity, k' rel = 151. o Disappearance half-life, T 50V , after subcutaneous injection in pigs : 14.4 ± 1.5 h (n = 5) . EXAMPLE 6 Synthesis of (N(1-carboxytridecyl) -2-amidosuccinyl) -Phe αB1 des(B30) human insulin. 5 Al,B29-diBoc-des (B30) human insulin (200 mg, 0.033 mmol) was dissolved in DMF (15 ml) and triethylamine (20 μl) was added. N ( 1 -carbomethoxytridecyl ) -2 -amidosuccinic acid N- hydroxysuccinimide ester (16 mg, 0.033 mmol) was added and 0 after 4 hours at room temperature the reaction mixture was evaporated in vacuo to dryness . The Boc groups were removed by treatment for 30 min at room temperature with trifluoroacetic acid (5 ml) . The trifluoroacetic acid was removed by evaporation in vacuo . The residue was dissolved at 0°C in 0.1 N NaOH (20 ml) . The saponification of the methyl ester was accomplished after 1 hour at 0°C. The pH value of the reaction mixture was adjusted to 5.0 by acetic acid and ethanol (5 ml) was added. The precipitate formed was isolated by centrifugation. The title compound was purified from the precipitate by ion exchange chromatography using a 2.5 x 27 cm column of QAE- Sephadex ® A25. The precipitate was dissolved ethanol/water (60:40, v/v) (25 ml) by adjustment of the pH value to 9.8 using ammonia and the solution was applied to the column. Elution was carried out in NH 3 /NH 4 C1 buffers at pH 9.0, using a linear gradient from 0.12 to 0.18 M NH.C1 in ethanol/water (60:40, v/v) and a total of 1000 ml of eluent . The UV absorbance of the eluate was monitored at 280 nm and fractions of 10 ml were collected. The title compound emerged from the column in fractions 62 to 72. The title compound was precipitated by diluting the pool with 2 volumes of water and adjusting the pH value to 5.0. After centrifugation the precipitate was washed with water and after a second centrifugation the product was dried in vacuo . Yield: 10 mg. Molecular weight, found by PDMS : 6032, theory: 6032. Relative lipophilicity, k' rel = 140. Disappearance half-life, T 50% , after subcutaneous injection in pigs: 8.65±1.65 hours (n = 5) . EXAMPLE 7 Phe αB1 -tetradecanoyl-glutamyl-glycyl des(B30) human insulin. Al,B29-diBoc des(B30) human insulin (200 mg, 0.033 mmol) was dissolved in DMF (15 ml) and triethylamine (100 μl) was added. Myristoyl-Glu (γ-OtBu) -Gly N-hydroxysuccinimide ester (95 mg, 0.17 mmol) was added and after 4 hours at room temperature the reaction mixture was evaporated to dryness in vacuo . The Boc and tBu groups were removed by treatment for 30 min at room temperature with trifluoroacetic acid (5 ml) . The trifluoroacetic acid was removed by evaporation in vacuo . The title compound was purified from the precipitate by RP-HPLC using a C18 silica column and eluting with a linear gradient s from 16 to 64% acetonitrile in a 50 mM Tris/phosphate buffer containing 75 mM (NH 4 ) 2 S0 4 at pH 7. The title compound emerged from the column at about 50% acetonitrile. The acetonitrile was evaporated in vacuo , and ethanol was added to 20% (v/v) . Adjustment of the pH value to 5.0 caused the product to o precipitate. After centrifugation the precipitate was dissolved in 10 mM NH 4 HC0 3 , desalted by gel filtration using Sephadex G-25 and lyophilized. Yield: 97 mg. Molecular mass, found by PDMS : 6105, theory: 6104. EXAMPLE 8 s Synthesis of Gly 328 ,Thr 329 , Lys 330 (N £ -tetradecanoyl) human insulin. 8a. Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Thr-Lys-Ser-Asp-Asp-Ala-Arg-A(l-21) insulin precursor from yeast strain yKV195 using the S . cerevisiae MF 0 alpha prepro-leader. The following oligonucleotides were synthesised: #4790 5 ' -TTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTCTAC ACTGGTACCAAGTCTGACGATGCTAGAGGTATTGTCG-3' #2371 5' -TTAATCTTAGTTTCTAGAGCCTGCGGG-3 ' The DNA encoding Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Thr-Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) was constructed in the same manner as described in Example 2 by substituting oligonucleotide #3881 with oligonucleotide #4790. The resulting plasmid was designated pKV195 and the yeast strain expressing Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Thr-Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) was designated yKV195. 8b. Isolation of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Thr- ys-Ser-Asp-Asp-Ala-Arg-A (1-21) insulin precursor. The yKV195 strain was fermented for 72 hours and 4.4 litres of broth were collected. Yeast cells were removed by centrifugation, the pH value was adjusted to 3.0 using sulphuric acid and 3.6 litres of water were added to dilute salts to a conductivity of 7.7 mS/cm. The insulin precursor was concentrated using a Pharmacia Strealine ® 50 column packed with 300 ml of Streamline ® SP ion exchanger. After wash with 3 litres of 25 mM citrate buffer, pH 3.6, the precursor was eluted using 0.5 M ammonia and the fraction from 300 to 600 ml was collected. Free ammonia was evaporated in vacuo at room temperature and the pH value of the resulting 280 ml was adjusted to 9.0 with hydrochloric acid. 8c. Synthesis of Ala-Thr-Arg-B (1-27) -Gly-Thr-Lys, Ser-Asp-Asp- Ala-Arg-A(l-21) insulin. To the 280 ml of solution containing 300 mg of the single-chain precursor were added 3 ml of immobilized A. lyticus protease gel (see PCT/DK94/00347, page 45) . After gentle stirring for 17 hours at 30°C the gel was removed by filtration. The pH value was adjusted to 3.5 and the solution was filtered though a Milipore ® 0.45 μ filter. The double-chain, extended insulin was purified in 3 runs by RP-HPLC using a 2x20 cm column packed with 10 μ spherical C18 silica of 120 A pore size and 0.2 M Na 2 S0 4 , 0.04 M H 3 P0 4 , pH 3.5 as buffer, and using a gradient from 23 to 33% acetonitrile at a rate of 4 ml/min and a column temperature of 40°C. The double-chain, extended insulin eluted at about 30-31% acetonitrile. The acetonitrile was removed from the combined pools of 70 ml by evaporation in vacuc, and the salt were removed by gelfiltration using a 5x47 cm column of Sephadex ® G-25 and 0.5 M acetic acid. The double-chain, extended insulin was isolated by lyophilization. Yield: 176 mg. 8d. Synthesis of N oA - 5 ,N αB"3 ,N eB30 -tris (tetradecanoyl) Ala-Thr-Arg- s B (1-27) -Gly-Thr-Lys, Ser-Asp-Asp-Ala-Arg-A(1-21) insulin. 176 mg of the double-chain, extended insulin was dissolved in a mixture of 1.4 ml of DMSO and 0.275 ml of 2 M diisopropylethylamine in NMP. The solution was cooled to 15°C and 0.963 ml of 0.3 M tetradecanoic acid N-hydroxysuccinimide o ester in DMSO/NMP (1:1, v/v) was added. After 20 hour at 15°C the reaction was stopped by addition of 50 ml of 0.01 M glycine buffer in ethanol/water (60:40, v/v) and the pH value adjusted to 10.0. The triacylated intermediate was not isolated. 8e. Synthesis of Gly B28 , Thr B29 , Lys 330 (N'-tetradecanoyl) human s insulin. To the solution from the previous step was added 2.5 ml of immobilized trypsin gel (see PCT/DK94/00347, page 46) . After gentle stirring at 15°C for 5 hours, the gel was removed by filtration, the pH value adjusted to 9.0 and the solution applied to a 1.5 x 26.5 cm column of QAE-Sephadex 1 A-25. Isocratic elution was performed at a rate of 9.3 ml/h using a 0.12 M NH 4 C1 buffer in ethanol/water (60:40, v/v) adjusted to pH 9.0 with ammonia. The title compound emerged from the column after 325-455 ml, peaking at 380 ml. Finally, the buffer was changed to 0.01 M NH 4 HC0 3 by gel filtration using Sephadex ® G-50 Fine, and the product isolated in the dry state by lyophilization. Yield: 50 mg. Molecular mass of the title compound, found by MS: 5979 ± 6, theory: 5977. Molecular mass of the B-chain, found by MS: 3600 ± 4, theory: 3600. Molecular mass of the C-terminal fragment of B-chain digested by V8 protease, found by MS: 1286 ± 2, theory: 1286. Relative lipophilicity, k' rel = 103. Disappearance half-life, T 50 , after subcutaneous injection in pigs : 17 ± 2 h (n = 4) . EXAMPLE 9. Synthesis of Gly 328 , Lys 329 (N £ -tetradecanoyl) human insulin. 9a. Synthesis of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) insulin precursor from yeast strain yKV196 using the S . cerevisiae MF alpha prepro-leader. The following oligonucleotides were synthesised: #4791 5' -TTGGTTGAAGCTTTGTACTTGGTTTGCGGTGAAAGAGGTTTCTTCTAC ACCGGTAAGTCTGACGATGCTAGAGGTATTGTCG-3' #2371 5' -TTAATCTTAGTTTCTAGAGCCTGCGGG-3 ' The DNA encoding Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) was constructed in the same manner as described in Example 2 by substituting oligonucleotide #3881 with oligonucleotide #4791. The resulting plasmid was designated pKV196 and the yeast strain expressing Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Lys-Ser-Asp-Asp-Ala-Arg-A (1-21) was designated yKV196. 9b. Isolation of Glu-Glu-Ala-Glu-Ala-Glu-Ala-Glu-Pro-Lys-Ala- Thr-Arg-B (1-27) -Gly-Lys-Ser-Asp-Asp-Ala-Arg-A(1-21) insulin precursor. The yKV196 strain was fermented for 72 hours and 3.6 litres of broth were collected. Yeast cells were removed by centrifugation, the pH value was adjusted to 3.0 using sulphuric acid and 3.4 litres of water were added to dilute salts to a conductivity of 7.7 mS/cm. The insulin precursor was concentrated to 300 ml using the procedure described in Example 8b. 9c. Synthesis of Ala-Thr-Arg-B (1-27) -Gly-Lys, Ser-Asp-Asp-Ala- Arg-A(l-21) insulin. To the 300 ml solution at pH 9.0 containing 390 mg of the single-chain precursor were added 5 ml of immobilized A. lyticus protease gel (see PCT/DK94/00347, page 45) . After gentle stirring for 40 hours at 30°C, the gel was removed by filtration. The pH value was adjusted to 3.5 and the solution was filtered though a Milipore ® 0.45 μ filter. The double- chain, extended insulin was purified in 3 runs by RP-HPLC using a 2x20 cm column packed with 10 μ spherical C18 silica of 120 A pore size and 0.2 M Na 2 S0 4 , 0.04 M H 3 P0 4 , pH 3.5 as buffer, and using a gradient from 23 to 33% acetonitrile at a rate of 4 ml/min and a column temperature of 40°C. The double-chain, extended insulin eluted at about 29% acetonitrile. The acetonitrile was removed from the combined pools of 60 ml by evaporation in vacuo, and the salt were removed by gelfiltration using a 5x47 cm column of Sephadex ® G-25 and 0.5 M acetic acid. The double-chain, extended insulin was isolated by lyophilization. Yield: 154 mg. 9d. Synthesis of N^ "5 ,N αB"3 ,N eB29 -tris (tetradecanoyl) Ala-Thr-Arg- B (1-27) -Gly-Lys, Ser-Asp-Asp-Ala-Arg-A(1-21) insulin. 154 mg of the double-chain, extended insulin was dissolved in a mixture of 1.05 ml of DMSO and 0.329 ml of 2 M diisopropylethylamine in NMP. The solution was cooled to 15°C and 0.22 ml of 0.3 M tetradecanoic acid N-hydroxysuccinimide ester in DMSO/NMP (1:1, v/v) was added. After 2 hour at 15°C, the reaction was stopped by addition of 40 ml of 0.01 M glycine buffer in ethanol/water (60:40, v/v) and the pH value adjusted to 10.0. The triacylated intermediate was not isolated. 9e. Synthesis of Gly 328 ,Lys 329 (N £ -tetradecanoyl) human insulin. To the solution from the previous step was added 1.5 ml of immobilized trypsin gel (see PCT/DK94/00347, page 46) . After gentle stirring at 15°C for 21 hours, the gel was removed by s filtration, the pH value adjusted to 9.0 and the product in 43 ml solution was applied to a 1.5 x 26.0 cm column of QAE- Sephadex ® A-25. Isocratic elution was performed at a rate of 9.5 ml/h using a 0.12 M NH 4 C1 buffer in ethanol/water (60:40, v/v) adjusted to pH 9.0 with ammonia. The title compound o emerged from the column after 190-247 ml, peaking at 237 ml. Finally, the buffer was changed to 0.01 M NH 4 HC0 3 by gel filtration using Sephadex ® G-50 Fine, and the product isolated in the dry state by lyophilization. Yield: 67 mg. Molecular mass of the title compound, found by MS: 5877 ± 2, s theory: 5876. Molecular mass of the B-chain, found by MS: 3499 ± 3, theory: 3499. Molecular mass of the C-terminal fragment of B-chain digested by V8 protease, found by MS: 1184 ± 2, theory: 1185. o Relative lipophilicity, k' rei = 118.5. Disappearance half-life, T 50t , after subcutaneous injection in pigs : 25 ± 9 h (n = 4) . SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: (A) NAME: Novo Nordisk A/S s (B) STREET: Novo Alle (C) CITY: DK-2880 Bagsvaerd (E) COUNTRY: Denmark (G) TELEPHONE: +45 44448888 (H) TELEFAX: +45 44490555 o (I) TELEX: 37173 (ii) TITLE OF INVENTION: ACYLATED INSULIN (iii) NUMBER OF SEQUENCES: 2 (iv) CORRESPONDENCE ADDRESS: s (A) ADDRESSEE: Novo Nordisk A/S Corporate Patents (B) STREET: Novo Alle (C) CITY: DK-2880 Bagsvaerd (E) COUNTRY: Denmark o (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.25 5 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (vii) PRIOR APPLICATION DATA: 0 (A) APPLICATION NUMBERS: DK 0276/95 (B) FILING DATES: 17-MAR-1995 (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Jørgensen, Dan et al . (C) REFERENCE/DOCKET NUMBER: 4341-WO,DJ 5 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: +45 44448888 (B) TELEFAX: +45 44493256 (2) INFORMATION FOR SEQ ID NO: 1 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 amino acids (B) TYPE: amino acid s (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1 : Gly lie Val Glu Gin Cys Cys Thr Ser lie Cys Ser Leu Tyr Gin Leu 1 5 10 15 o Glu Asn Tyr Cys Xaa 20 (2) INFORMATION FOR SEQ ID NO:2 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 30 amino acids s (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2 : Xaa Xaa Xaa Gin His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr o 1 5 10 15 Leu Val Cys Gly Glu Arg Gly Phe Phe Xaa Xaa Xaa Xaa Xaa 20 25 30

Claims: CLAIMS 1. An insulin derivative having the following sequence: A-Chain S S I 7 i s Gly-lie-Val-Glu-Gin-Cys-Cys-Thr-Ser-lie-Cys-Ser- 1 2 3 4 5 6 I 8 9 10 11 12 S B-Chain S I Xaa -Xaa -Xaa -Gln-His -Leu -Cy Is -Gly- Ser -His -Leu -Val - 1 2 3 4 5 6 7 8 9 10 11 12 A-Chain (contd. ) 20 5 Leu- •Tyr- -Gln- -Leu- -Glu- -Asn- -Tyr- -Cys - -Xaa ;SEQ ID NO:l) 13 14 15 16 17 18 19 1 21 r " — S B-Chain (contd.) Glu-Ala-Leu-Tyr-Leu-Val-Cy Is-Gly-Glu-Arg-Gly-Phe- 13 14 15 16 17 18 19 20 21 22 23 24 B-Chain (contd.) Phe-Xaa-Xaa-Xaa-Xaa-Xaa (SEQ ID NO: 2) 5 25 26 27 28 29 30 wherein Xaa at position A21 is any codable amino acid except Lys, Arg and Cys; Xaa at positions Bl, B2 , B3, B26, B27, B28 and B29 are, o independent of each other, any codable amino acid except Cys or deleted; Xaa at position B30 is any codable amino acid except Cys, a dipeptide comprising no Cys or Arg, a tripeptide comprising no Cys or Arg, a tetrapeptide comprising no Cys or Arg or 5 deleted; and either the amino group of the N-terminal amino acid of the B-chain has a lipophilic group, W, attached to it which group has from 12 to 40 carbon atoms and optionally contains a group which can be negatively charged or the carboxyl group of the C-terminal amino acid of the B-cham has 48 a lipophilic group, Z, attached to it which group has from 12 to 40 carbon atoms and optionally contains a group which can be negatively charged with the proviso that if one or more of the amino acids at position Bl, B2 and B3 is (are) deleted then the s number of the N-terminal amino acid is found by counting down from Cys B7 which is always assigned the number 7 and that (a) when B1-B2-B3 is Phe-Val-Asn and A21 is Asn and B26-B27- B28-B29-B30 is Tyr-Thr-Pro-Lys-Thr or Tyr-Thr-Pro-Lys-Ala, then said W or Z always contains a group which can be negatively o charged; and (b) when B29 and B30 are deleted and a group Z as defined above is present at the C-terminal amino acid of the B-chain and neither Bl, B2 nor B3 is deleted then B1-B2 is different from Phe-Val or B26-B27-B28 is different from Tyr-Thr-Pro or both s B1-B2 and B26-B27-B28 are different from said sequences; and (c) when B29 and B30 are deleted and a group Z as defined above is present at the C-terminal amino acid of the B-chain and one of Bl, B2 or B3 is deleted then the N-terminal amino acid of the B-chain is different from Val or the sequence B26-B27-B28 0 is different from Tyr-Thr-Pro or both the N-terminal amino acid of the B-chain and the sequence B26-B27-B28 are different from Val and Tyr-Thr-Pro respectively. 2. The insulin derivative according to claim 1, wherein a lipophilic group, W, is attached to the amino group of the N- 5 terminal amino acid in the B-chain. 3. The insulin derivative according to claim 1, wherein a lipophilic group, Z, is attached to the carboxyl group of the C-terminal amino acid in the B-chain. 4. The insulin derivative according to any one of the preceding o claims, wherein Xaa at position A21 designates an amino acid selected from the group comprising Ala, Asn, Gin, Glu, Gly and Ser. 49 5. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position Bl designates Phe or is deleted. 6. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position B2 designates Ala or Val. s 7. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position B3 designates an amino acid selected from the group comprising Asn, Gin, Glu, and Thr. 8. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position B26 designates Tyr. o 9. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position B27 designates Thr. 10. The insulin derivative according to any one of the claims 1 to 3 , wherein Xaa at position B28 designates Pro. 11. The insulin derivative according to any one of the claims s 1 to 3, wherein Xaa at position B29 designates Lys or Thr. 12. The insulin derivative according to any one of the claims 1 to 3 , wherein Xaa at position B30 designates Thr or e- acylated Lys. 13. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position B30 is deleted. 14. The insulin derivative according to any one of the claims 1 to 3 , wherein Xaa at position B28 designates Lys and Xaa at position B29 designates Pro. 15. The insulin derivative according to any one of the claims 1 to 3, wherein Xaa at position B28 designates Pro and Xaa at position B29 designates Thr. 16. The insulin derivative according to claim 2 wherein W is attached to the amino group of the N-terminal amino acid via an amide bond. 17. The insulin derivative according to claim 16 wherein W is s CH 3 (CH 2 ) n CH(COOH)NH-CO(CH 2 ) 2 CO- and n is an integer from 9 to 15. 18. The insulin derivative according to claim 3 wherein Z is attached to the carboxyl group of the C-terminal amino acid via an amide bond. 19. The insulin derivative according to claim 18 wherein Z is ιo -NHCH (COOH) (CH 2 ) 4 NH-C0 (CH 2 ) m CH 3 and m is an integer from 8 to 18. 20. The insulin derivative according to claim 19 wherein the parent insulin to which Z is attached is Thr B29 human insulin. 21. The insulin derivative according to claim 19 wherein the parent insulin to which Z is attached is des(B28-B30) human s insulin. 22. The insulin derivative according to claim 19 wherein the parent insulin to which Z is attached is des(B27-B30) human insulin. 23. The insulin derivative according to claim 19 wherein the 20 parent insulin to which Z is attached is des(B26-B30) human insulin. 24. An insulin derivative according to claim 1 wherein the C- terminal amino acid of the B-chain is e-acylated Lys and the amino acid next to the C-terminal amino acid is Gly. 25 25. A pharmaceutical composition for the treatment of diabetes in a patient in need of such treatment, comprising a therapeutically effective amount of an insulin derivative according to claim 1 together with a pharmaceutically acceptable carrier. 26. A pharmaceutical composition for the treatment of diabetes in a patient in need of such treatment, comprising a therapeutically effective amount of an insulin derivative according to claim 1, in mixture with an insulin or an insulin s analogue which has a rapid onset of action, together with a pharmaceutically acceptable carrier. 27. A method of treating diabetes in a patient in need of such a treatment, comprising administering to the patient a therapeutically effective amount of an insulin derivative o according to claim 1 together with a pharmaceutically acceptable carrier. 28. A method of treating diabetes in a patient in need of such a treatment, comprising administering to the patient a therapeutically effective amount of an insulin derivative s according to claim 1 in mixture with an insulin or an insulin analogue which has a rapid onset of action, together with a pharmaceutically acceptable carrier.

Inventors: BRANDT JAKOB, HANSEN PER HERTZ, HAVELUND SVEND, JONASSEN IB, KAARSHOLM NIELS CHRISTIAN, KURTZHALS PETER, MARKUSSEN JAN, BRANDT, JAKOB, HANSEN, PER, HERTZ, HAVELUND, SVEND, JONASSEN, IB, KAARSHOLM, NIELS, CHRISTIAN, KURTZHALS, PETER, MARKUSSEN, JAN
Applicants: BRANDT JAKOB, HANSEN PER HERTZ, HAVELUND SVEND, JONASSEN IB, KAARSHOLM NIELS CHRISTIAN, KURTZHALS PETER, MARKUSSEN JAN, NOVONORDISK AS, BRANDT, JAKOB, HANSEN, PER, HERTZ, HAVELUND, SVEND, JONASSEN, IB, KAARSHOLM, NIELS, CHRISTIAN, KURTZHALS, PETER, MARKUSSEN, JAN, NOVO NORDISK A/S
Application year: 1996
Main classification: C07K14
IPC codes: A61K38, A61P9, C07K14, C12N15, C12P21, C12R1