IgG SEPARATION MEDIUM AND NOVEL PROTEIN A VARIANT

Abstract: A separation medium comprising a base matrix and matrix-bound groups which exhibit recombinant Protein A containing a cysteine. The groups comply with the formula: - B - X - rProtein A-cys where B is a bridge which binds to the base matrix and X includes a heteroatom N or S from rProtein A-cys. The characterizing feature is that X is thioether sulphur and/or a secondary amine (-NH-). Protein A variant characterized in that the C-terminal residue is cysteine.

Description: IgG separation medium and novel Protein A variant Adsorbents which exhibit IgG-binding proteins have been used to capture IgG in aqueous media for more than twenty years. Initially, native Protein A (GB 1,441,979 (Sjδqvist) ) was used. Later recombinantly produced forms of Protein A and Protein G (WO 8400773) (Lδfdahl, et al) and EP 262,192 (Guss, et al) and U.S. 5,082,773 (Fahnestock) were developed. Protein A has a broad IgG-specificity with respect to animal species, but the specificity may vary with respect subclasses (for instance, human IgG3 will not bind to Protein A) . Protein G binds to all IgG subclasses of a majority of important mammalian species . The advantage of Protein A compared to Protein G is that the binding of IgG is weaker, and consequently milder conditions can be used to release IgG from Protein A. This is of importance for the purification of individual monoclonal antibodies. Recombinant techniques enable simple mapping of IgG-binding proteins with regard to the functionality of different domains. In the case of Protein A, it was found that the native form contained five consecutively ordered IgG-binding domains (E, D, A, B and C) followed by an X-domain which did not bind IgG. The new technique facilitated the preparation of IgG-binding fragments and variants where one or more amino acids was/were replaced, added or removed. Unless otherwise indicated, reference to Protein A indicates the native form or IgG-binding fragments and variants of Protein A that have the same IgG specificity as native Protein A. Variants of Protein A which contain cysteine were produced relatively early on, and the cysteine residue inserted was used for binding to base matrices. It was considered important not to place cysteine as a C-terminal residue. A variant having cysteine as the penultimate amino acid in the C-terminal part was bonded to activated Thiol Sepharose® (Pharmacia Biotech AB, Uppsala, Sweden) via disulfide bond formation and studied as an IgG- separation medium (T. Profy (Repligen) ; EP 284,368 and U.S. 5,084,559) . Similar studies were also presented in FASEB 87, 29 March-2 April 1987 (Poster N44, Profy, et al) . The results obtained with three other variants (1, 2 and 5 domains) of Protein A with cysteine in a C-terminal linker sequence (amino acid 10 from the C-terminal) (Ljungquist, et al, Eur. J. Biochem. 186 (1989) 557-561) were later presented. These latter variants were also coupled covalently via disulfide bound formation to thiopropyl Sepharose®. Immobilization to tresyl chloride or tosyl chloride activated gels was suggested as an alternative, with the intention of avoiding reductively sensitive linking groups. An equimolar relationship was found between IgG binding capacity and the number of domains for one-domain and two-domain variants. The five-domain variant never bonded more than the double molar amount of IgG. IgG- capacities comparable with those earlier achieved with soluble forms of native Protein A were achieved (it was later found that in .certain applications, non-cys-containing variants can give molar binding ratios which lie between two and three) . Parallel herewith, Genex (U.S. 4,977,247 (Fahnestock, et al) have produced a recombinant variant of rProtein G-cys in which cysteine is seated in the C-terminal position in an IgG- binding domain. In preparing separation media based on this Protein G variant, the choice was to bind rProtein G-cys covalently to a inohexyl-agarose activated with the bi¬ functional reagent N-sulfosuccinimidyl-4- (p-maleimidophenyl) butyrate (U.S. 4,977,247, Claim 1 and column 18, lines 22-37) . Gam aBindG® Plus (Pharmacia Biotech AB) is a commercially available εolid phase rProtein G-cys product with cysteine as the C-terminal residue. The product is synthesized by coupling the cysteinyl residue to aminohexyl agarose activated with N-sulfosuccinimidyl 4- (N-maleimidomethyl) cyklohexane-1- carboxylate. As far as we are aware, the variant of the matrix-bound rProtein A-cys produced by Repligen has not found favor commercially. The reason may be that the coupling to the matrix is through unstable structures (-S-S-) , although the reason may also be due to factors unknown to us. Whatever the reason, however, the adsorbent that totally dominates commercially makes use of native Protein A or different forms of recombinant Protein A that lack cysteine. The market for products based on Protein G has been substantially smaller, probably because Protein A has more advantageous binding properties. OBJECT OF THE INVENTION The object of the invention can be summarized in the desire to provide adsorption media which have a) the IgG-binding specificity of Protein A; b) at least the same stability as other adsorbents based on native Protein A; c) the same or improved capacity to bind IgG compared to earlier known variants of matrix bound rProitein A-cys (primarily calculated as the ratio mol IgG per mol cys-variant of Protein A with one, two or more IgG binding domains) . For variants with two or more domains this means molar ratios > 2. Fulfillment of these objects will enable more effective processes to be used for cleansing IgG from different starting materials. THE INVENTION The main aspect of the invention is a separation medium which comprises a base matrix substituted with the groups complying with formula I: - B - X - rProtein A-cys, I where a. rProtein A-cys is recombinantly produced Protein A which contains cysteine in its amino acid sequence; b. B is a bridge which binds to the base matrix; and c. X contains an heteroatom N or S originating f om rProtein A-cys. The characteristic feature is that X is thioether sulphur (- S-) and/or secondary amine (-NH-) , i.e. in one and the same separation medium X may be either or both of thioether sulphur or/and secondary amine, with preference for 50 %, such as essentially 100 %, of all X being thioether sulphur. The optimal molar ratio between the total IgG binding capacity the amount of IgG on the matrix may vary in dependence on the number of IgG-binding domains that are present in the Protein A of the adsorbent. For single-domain variants the ratio is 1 and for 2-domain variants the ratio iε = 2. For three-, four- and five-domain variants the ratio is = 2 or preferably > 2. The maximum value is determined by the number of IgG-binding domains and is therewith contingent on the Protein A construction used. In this aspect of the invention, B can, in principle, be anything that has the satisfactory stability under the conditions applied in the adsorption/desorption of IgG (time, temperature, pH, etc.) . Examples of relevant structureε in the bridge -B- are amide, ester, ether, thioether, hydrocarbon chains, azo, carbamate, etc. Hydrocarbon chains present in -B- may be straight, branched or cyclic and normally have only saturated carbon atoms (2-10 carbon atoms, preferably 2, 3 or 4 carbon atoms to retain a pronounced hydrophilic nature) . It is preferred that the bridge -B- binds to the base matrix via an ether structure or an amide/ester structure. It is also preferrred that B compriseε a straight branched or cyclic saturated hydrocarbon chain which may optionally be broken by one or more oxygen/nitrogen atoms and substituted with one or more amino or hydroxy groups.For stability reasonε one and the εame carbon atom εhould bind at most one oxygen or nitrogen atom. The structureε that are absolutely preferred in -B- are thoεe which occur when rProtein A-cyε iε coupled to the matrix via an epoxy group or epihalo group, i.e. B presentε in its right-hand terminal (nearest X) the structure -CH 2 -CHOH-CH 2 - X becomes a secondary amine or thioether, depending on whether ε-amino group in lysine or the N-terminal amino group, or a thiol group in cysteine is coupled. The aforesaid bridge structures can be formed in accordance with current techniques, for instance by the use of bi¬ functional coupling reagents, such as epichlorohydrin, bisepoxide (such as 1,4-bis (2,3-epoxypropoxy) butane, N- εulfoεuccinimidyl 4- (N-maleimidomethyl) -cyklohexane-1- carboxylate, etc. Relevant base matrices may be activated with such reagents, so that they will contain groups that reacts more or less selective with thiol or amino groups . Preferred coupling reagents and conditions gives very little coupling at primary amino groups (ε-amino in lysyl and the N-amino terminal) . Relevant forms of rProtein A-cys have an amino acid in the native sequence replaced with cysteine. Alternatively, cysteine can be present in an amino acid seguence (linker) which has been fuεed to a terminal, or aε an inεert in the native sequence or an IgG-binding part thereof. Cyεteine may alεo be included in a peptide linker that preferably is N- terminal or C-terminal to an IgG-binding domain. Generally εpeaking, a terminal cyεteine iε preferred to an internal cyεteine. The length of the linker used is normally not critical and may vary from one to fifty amino acid residues, for instance. For all cysteine modifications it is imperative that the IgG bindig ability shall not get lost. rProtein A-cys may also be modified in other ways. For example, rProtein A-cys may be a fusion protein which, in addition to the IgG-binding domain from Protein A, also includes one or more IgG-binding domains from Protein G or from some other IgG-binding protein (cf. Gusε, et al, EP 262, 192) . The native domains may be per utated, occur one or more times, or some may be miεsing. Native non-IgG-binding domains may be missing totally or in part. rProtein A-cys can be prepared in analogy with current techniques (Profy T, EP 294,386; and Ljungquiεt, et al, Eur. J. Biochem. 186 (1989), 557-561) . The baεe matrix is a hydrophilic polymer which contains a plurality of amino groups and/or hydroxy groups, primarily the latter. The base matrix is normally insoluble in aqueous media. The base matrix may originate from a polysaccharide, such as dextran, cellulose, starch, agarose, pullulan, xylan, etc., which may be crosε-linked and/or provided with different groups suitable for the use intended. Among synthetic polymers can be mentioned polymers of hydroxyalkyl acrylates or corresponding methacrylates, polyvinyl alcohols, polymers of vinyl hydroxyalkyl ethers, etc. To the extent that a polymer is soluble, it can be made insoluble, for inεtance crosslinked or adsorbed or covalently bound to a support which iε inεoluble in aqueouε media, for inεtance a εtyrene divinyl benzene copolymer. The baεe matrix can alεo be in the form of particleε that may be more or leεε εpherical and/or porouε or non-porous . One particular type of matriceε iε porous hydrophobic particleε made of divinyl benzene-styrene copolymer or some other hydrophobic polymer/copolymer, the the inner and/or outer surfaces of which have been hydrophilized and provided with OH-groups. In one preferred type of embodiments, the matrix is normally insoluble in aqueous media, porous and based on a polysaccharide. Another main aspect of the invention involves binding (adsorbing) IgG to a separation medium. IgG is then contacted with a separation medium in accordance with the aforegoing. Adsorption is normally taking place from an aqueous εolution derived from serum or a cell culture capable of producing IgG. Suitable conditions lie in the range 0-35°C, pH 6-8, salt concentration 0.1-3 M (depending on the type of IgG to be bound) . Before desorption of bound IgG, the separation media are normally waεhed, suitably with a buffer essentially with the same pH as the adsorption buffer, whereafter desorption is effected conventionally, for instance by treatment with a buffer which has a pH beneath 5. The conditions should be non- denaturing. rProtein A-cys with cysteine as the C-terminal is novel. Binding of IgG to the inventive εeparation medium haε a broad fied of uεe. It can be utilized in proceεses involving capture of IgG from a solution, i.e. to separate IgG dissolved in an aqueous solution from other components present therein. Binding of IgG may be a part-step in a chromatography process or in a batch-wise procesε. Binding of IgG may alεo be a part of a so-called immunoassay or in an extracorporeal process for removing IgG from whole blood or plasma. The primary area of use iε found in purifying IgG (including monoclonal IgG antibodieε) . A very expedient embodiment of the invention iε to couple rProtein A-cys via a C-terminal cysteine to a chromatographic particulate matrix containing densifying filler particles, such as Anval® (Anval, Torshalla, Sweden) . The εo obtained chromatographic εupport haε been found very useful for chromatographic separations of IgG in stabilised fluidised bedε. See our contemporary patent application SE 9503926-9 relating to "Adεorption Method and Separation Medium" (incorporated by reference) . With regard to chromatography on expanded/fluidized bedε, reference iε made to WO 9218237 (Pharmacia Biotech AB) . EXPERIMENTAL PART Thθ preparation of rProtein λ-cys rProtein A-cys waε prepared analogously with the description given in EP 284,368 or by Ljungqvist, et al, Eur. J. Biochem. 186 (1989), 557-561. The sequence was the same as that diεclosed in EP 284,365, with the exception that the firεt 18 amino acids were misεing (εignal sequence) and that the last 103 amino acids were replaced with an hexapeptide sequence which has cysteine as C-terminal. Coupling of rProtein λ-cys to base matrix Activating with the aid of 1, -bis (2 , 3-epoxypropoxy) butane (BPR-butane) . One litre of drained Sepharose® FF (agarose in bead form croεε-linked with epichlorohydrin, Pharmacia Biotech AB, Uppεala, Sweden) waε waεhed on a filter funnel with distilled water and admixed with 55 g NaOH dissolved in 300 ml distilled water, 35°C, in a thermostat-controlled reaction vessel while stirring the syεtem. 390 ml BPR-butane were added. The εystem was stirred for two hours at 35°C, followed by washing with 15 1 water. Coupling of rProtein A-cvs. The activated gel waε washed on a filter funnel with 3x1 1 nitrogen-gas saturated 0.1 M Na- phosphate, 1 mM EDTA pH 8.5, and was allowed to drain. The gel was then mixed with 5.5 g rProtein A-cyε diεεolved in a nitrogen-gas saturated aqueous solution of 0.1 M Na-phoεphate, 1 mM EDTA pH 8.5. The εyεtem waε εtirred at 37°C while blowing in nitrogen gas. Sodium sulphate (370 g) waε added. After εtirring the εyεtem for two hourε at 37°C, the gel waε waεhed with 3 1 distilled water and drawn-off by εuction. Deactivation. The drawn-off gel waε mixed with 100 ml thioglycerol diεεolved in 900 ml 0.2 M εodium bicarbonate, 0.5 M NaCl, 1 mM EDTA pH 10, while εtirring the εyεtem. The εystem was stirred overnight at 37°C, whereafter the gel was washed on a filter funnel with 0.1 M Tris, 0.15 M NaCl, pH 8, and 0.05 M acetic acid in three cycles with 3x1 gel volume in each cycle. The gel was washed finally with water. Determining the total capacity of human IgG Instrument: FPLC with superloop (Pharmacia Biotech AB) . Column: 1 ml HR 5/5 (Pharmacia Biotech AB) . Buffer A: 10 mM sodium dihydrogenphosphate, 0.15 M sodium chloride, 10 mM EDTA, pH 7. Buffer B: 0.5 M acetic acid (gives a pH of about 2.7) . IgG-solution: 150 mg human IgG in 10 ml buffer A (centrifuged and filtered) . Printer speed: 0.05-0.25 cm/min. 1.0 ml of drained gel was packed in the column and equili¬ brated with buffer A. The IgG-solution was delivered through the superloop at a flow rate of 0.15 ml/min., until the gel was saturated with respect to IgG. After washing with buffer A at the same rate of flow, bound IgG was eluated with 9 ml buffer B at a flow rate of 0.30 ml/min. The eluate with buffer B was collected and its volume determined (weighed) . A,,, was read-off after diluting to 1:10. The formula applied in determining the IgG-capacity was: Eluate volume in ml x A 3M on dilute eluate x 7.244 = mg IgG/ l of drained gel. Determining the breakthrough capacity Q, for human IgG Column: XK 16/20 (Pharmacia Biotech AB) . Buffer A: 20 mM Na-phosphate, pH 7.0. Buffer B: 0.1 M glycine, pH 3.0. IgG-solution: About 0.5 g IgG per 1 in Buffer A. Flow rate: 10 ml/min. (300 cm/h) . Printer speed: 0.02 cm/ml. Column volume: 23 ml. Delivery of IgG solution was interrupted when c/c 0 measured in the eluate had reached 1% (c and c 0 are protein concentra¬ tions in eluent εubsequent to and prior to passage of the column) . Adsorbed IgG was then eluated with buffer B and its volume determined as mg IgG per ml of drained gel. Results and conditions for the coupling experiment that gave the highest dynamic capacity were: Na-sulphate 1.3 M; charged quantity of rProtein A-cys 7.1 mg/ml gel; coupling buffer pH 8.5; coupling temperature 37°C; coupling time 2 hourε; total capacity 52.2 mg IgG per ml gel; breakthrough capacity 31.3 mg per ml gel at c/c 0 = 1%. Largely comparable dynamic capacitieε could be obtained in trialε where 5-6 mg rProtein A-cyε were charged for each ml gel. Other coupling methods Epoxy-coupling of rProtein A-cys was compared with native Protein A (lacks cysteine) coupled to N-hydroxysuccinimide (NHS) and rProtein A-cyε coupled to N-sulfosuccinimidyl 4- (N- maleimidomethyl) cyclo-hexane-1-carboxylate (Sulfo-SMCC) . In coupling native Protein with NHS or epoxide, coupling is effected εolely via an amino group. In the case of the reagent Sulfo-SMCC, coupling of rProtein A-cys iε effected via a thiol group. In coupling rProtein A-cyε with epoxy (BPR), coupling can be effected both to a thiol group and to an amino group, the preference being determined by pH. At comparable degreeε of substitution, the total capacity increased in the order NHS, epoxy, Sulfo-SMCC. The differences are probably due to stearic effects caused by amine coupling via groups that are not seated terminally. Comparison tests with rProtein G and rProtein G-cys are also reported below. Table 1. Capacity of human IgG for different coupling methods applied on Sepharose® FF 1 ) Subst deg Tot cap Q B Mol IgG/ nmol/ml mglgG/ml gel molProt A gel 387758A 21 NHS Prot A 104 23.8 ND 31 1.5 387789 21 SulfoSMCC, rProt A-cys 100 44.7 32 2.9 441713A1 2 ' Epoxy, rProt A-cys 100 35.2 ND 31 2.3 Table 2. Capacity of human IgG to bind to Protein G adsor¬ bents Subεt deg Tot cap Mol IgG/ nmol/ml gel mg IgG/ l mol Prot A 35169 2 ' GammaBindG® 1,4l Type 2 CNBr, Sepharose® 4B 1 ' 145 23.3 1.03 35170 ' GammaBindG® l,4, Type 3, SulfoSMCC, Sepharose® CL6B 1 220 41 . 5 1 . 2 35174 2 ' GammaBindG® 1 ' 4l Type 2 , NHS , Sepharose® 6FF 11 204 26 0 . 8 ^Pharmacia Biotech AB, "'internal j ournal number, 'Not specified, 4, GammaBindG Type 2 is Protein G and GammaBindG Type 3 iε Protein G with cyεteine aε C-terminal. Both variants have two IgG-binding domains. The results show that a very good total capacity and break¬ through capacity were achieved, and that the capacity in mol IgG per mol rProtein A-cys was far above that earlier achieved for cys-containing IgG-binding proteins. The breakthrough capacity for human IgG. Comparison studies for different Protein A adsorbents Methodology: Protein A matrices (rProtein A Sepharose® Fast Flow (this invention, immobilization via epoxy) ; Protein A Sepharose® 4 Fast Flow (immobilization via CNBr, Pharmacia Biotech AB) ; PROSEP A® (Bioprocessing Ltd. , UK) and Protein A Hyper D® (BioSepra S.A., France) were packed in XK 16/20 columnε to a bed height of 10 cm. The gels were equilibrated in 20 mM phosphate buffer, pH 7.4. A sample consisting of human polyclonal IgG (1 mg/ml) in the same buffer was delivered to respective gels in the linear flow 190 cm/h. Sample delivery was interrupted when the concentration of IgG in the eluate had reached 10% of the initial IgG concentration of the sample solution. Non-bound IgG was washed out and the bound IgG eluated with 0.1 M citrate, pH 3. The breakthrough capacity Q B was calculated as the amount of IgG that had bound per ml of gel when the IgG concentration in the eluate was 5% of the initial IgG concentration in the sample. The concentration of Protein A in the eluated IgG fraction was determined with ELISA. The amount of Protein A in the IgG fraction is given as ng protein A/mg IgG. Results: Protein A matrix Q 8 Protein A in (mg/ml) IgG-fraction (ng/mg) rProtein A-cys Sepharpse® FF 40 11 Protein A Sepharose® 4 FF 23 8 PROSEP A® 24 266 Protein A Hyper D® 27 Not analyzed. These values show that the invention enables the construction of Protein A adsorbents whose breakthrough capacities are higher than other commercially available matrices. Compared to the same matriceε, the stability with regard to the release of Protein A is roughly the εame or better.

Claims: CLAIMS 1. A separation medium comprising a base matrix and matrix- bound groups which exhibit recombinant Protein A containing a cysteine, said groups complying withthe formula - B - X - rProtein A-cys where B is a bridge which binds to the base matrix and X includes a heteroatom N or S from rProtein A-cys, characterized in that X is thioether sulphur (-S-) and/or a secondary amine (-NH-) . 2. The separation medium according to Claim 1, characterized in that the bridge - B - binds to the base matrix via an ether structure and iε compriεed of a εtraight, branched or cyclic saturated hydrocarbon chain which is optionally broken by one or more oxygen/nitrogen atoms and substituted with one or more amino groups or hydroxy groups, wherein one and the same carbon atom binds at most one oxygen atom or nitrogen atom. 3. The. separation medium according to one of Claims 1-2, characterized in that cysteine is included in a terminal peptide linker. 4. The separation medium according to any one of Claims 1-3, characterized in that the peptide linker iε C-terminal. 5. The εeparation medium according to any one of Claims 1-4, characterized in that cysteine is the C-terminal amino acid residue in rProtein A-cys. 6. The separation medium according to any one of Claims 1-5, characterized in that the base matrix is a polyhydroxy polymer, preferably an insolubilized polyεaccharide. 7. Recombinant Protein A-cyε, characterized in that the C- terminal reεidue is cysteine.

Inventors: JOHANSSON INGEMAR, JOHANSSON, INGEMAR
Applicants: JOHANSSON INGEMAR, PHARMACIA BIOTECH AB, JOHANSSON, INGEMAR
Application year: 1996
Main classification: C07K1
IPC codes: C07K1, C07K14, C07K16, C12N15, G01N33