PROCESS FOR REDUCING STEROLS IN EGGS

Abstract: A mixture of cyclodextrin, a base and water (10) are mixed with egg yolk (14) to reduce the cholesterol content of the egg yolk. The cholesterol is removed as a complex of cyclodextrin (24) while the base prevents loss of protein from the egg yolk. The amount of base added is sufficient to adjust the pH to between 7.5 and 12.

Description: PROCESS FOR REDUCING STEROLS IN EGGS BACKGROUND OF THE INVENTION (1) Field of the Invention This invention relates to a process for reducing sterols, especially cholesterol, in eggs through the use of cyclodextrins. (2) Description of Related Art Studies have linked sterols and, especially, cholesterol with increased rates of heart disease and certain types of cancer. As a result of these findings, there has been a demand by consumers and the food industry for reduced cholesterol foods. For example, chicken eggs have fallen into disfavor among consumers due to their high cholesterol content. Cholesterol is located in the yolk of an egg, and reduction of the cholesterol content in the yolk should improve consumer perception of eggs. It is known that sterols such as cholesterol will form complexes with cyclodextrin and that such complexes will form when cyclodextrin is mixed with egg yolks in the presence of water. It is also known that these complexes can be separated from the egg yolk by centrifugation, thereby reducing the cholesterol/sterol content of the egg yolk, see for example U.S. Patent Nos. 5,063,077; 5,223,295; and 5,292,546. U.S. Patent 5,223,295 teaches that water is mixed with the egg or egg yolk, and then, cyclodextrin is mixed with the water and egg/egg yolk. After mixing for a period of time the complex is removed and a reduced cholesterol egg/egg yolk is obtained. A problem associated with this process is that a significant portion of the protein in the egg yolk is also removed when the complex is separated from the yolk. This loss of protein yields an overall loss in the amount of egg yolk recovered after treatment, thereby, reducing the economic viability of the process. U.S. Patent No. 5,063,077 teaches separating the granules from the egg yolk prior to treating the egg yolk with cyclodextrin. After the complex has been removed, the granules are resuspended in the egg yolk. A disadvantage to this approach is that the separated granules contain a significant quantity of cholesterol. Resuspending the granules in the treated egg yolk increases the cholesterol content of the treated egg yolk, thus thwarting the whole purpose of the cyclodextrin treatment. U.S. Patent No. 5,292,546 teaches adding an aqueous solution of sodium chloride or ammonium carbonate to the yolk prior to adding the cyclodextrin to the egg yolk; and then subsequently removing the sodium chloride or ammonium carbonate. The disadvantage to this approach is that the salt and ammonium adversely affect the flavor profile of the egg yolk. Furthermore, salt has fallen into disfavor because of its link to high blood pressure. Removal of the salt from the egg yolk is costly. There is a need for a simple process for reducing sterols like cholesterol from eggs. Such a process should have a short treatment time and have minimal effect on the flavor profile and shelf life of the yolk. SUMMARY OF THE INVENTION A process has now been discovered which uses cyclodextrin to reduce sterols such as cholesterol from eggs without substantial loss of protein from the egg, and without the need to separate the egg yolk from the egg or the granules from the egg yolk. The process of the present invention provides an economic advantage to the user because the treatment time is short and the treatment can employ a low shear or mixing rate while still obtaining good results. The process of the present invention employs a food grade base to adjust the pH of the egg to between about 7.5 and 12 coupled with cyclodextrin in the presence of water to remove sterols like cholesterol from the egg yolk. Additionally, it has been found that a low shear rate can be employed to mix the cyclodextrin and base with the egg yolk. Furthermore, it has been found that after a relatively short time, about 1 to about 10 minutes, that a sufficient amount of sterols have complexed with cyclodextrin such that the sterol content of the egg has been substantially reduced. After removal of the complex from the egg, the pH of the egg yolk is adjusted back to normal, 6 to 7, by the addition of a food grade acid. These pH adjustments have been found to have little or no effect on the flavor profile or the shelf life of the treated egg. Broadly, the process of the present invention comprises the steps of: (a) forming a uniform liquid mixture comprising water, a food grade base, cyclodextrin, and egg yolk, at a temperature of about 20 β C to about 60"C, said food grade base being present in an amount sufficient to adjust the pH of said mixture to between about 7.5 and 12, said cyclodextrin and water being present in an amount sufficient to form complexes between the sterol and the cyclodextrins; (b) separating said complexes from said mixture; (c) adjusting the pH of said mixture with a food grade acid to about 6 to about 7; and (d) recovering an egg yolk with reduced sterol. The terms sterol and cholesterol as used in the specification and claims mean not only sterols and cholesterol per se but also their esters. Reference is made to treating egg yolk, however the egg yolk may be in the form of egg yolk separated from the white or may be in the form of a whole egg, i.e. yolk and white together. Good results have been obtained with egg yolk separated from the egg white. Reference is made in the specification and claims to removing the cholesterol from egg yolks because the cholesterol is contained in the yolk of the egg and not the white. Any bird egg can be treated in accordance with the present invention, for example, ostrich, chicken, pigeon, duck, goose or turkey. Chicken eggs are the most common in the market place today. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 illustrates the process of the present invention in a preferred batch mode; Figure 2 illustrates the process of the present invention in a preferred continuous mode; and Figure 3 illustrates another preferred embodiment of the present invention. DESCRIPTION OF THE PREFERRED EMBODIMENTS Suitable food grade bases for use in the present invention are alkali metal cation containing food grade bases. Specifically, food grade bases that contain the alkali metal cation of sodium or potassium are preferred for use in the present invention. More specifically, suitable alkali metal cation containing food grade bases include sodium hydroxide, potassium hydroxide, sodium carbonate and potassium carbonate. It is preferred to use a food grade base which does not contain sodium because of the link between sodium and high blood pressure. Thus, potassium hydroxide and potassium carbonate are most preferred for use in the present invention. Good results have been obtained with potassium hydroxide. The amount of food grade base used in the process of the present invention is enough to adjust the pH of the mixture to between about 7.5 and 12 and more preferably to about 8.5 to 10.5. Good results have been obtained with a pH of about 9 to 10. The pH of the solution is determined in a conventional manner using conventional equipment such as a pH meter. When using potassium hydroxide, the amount of base in solution should be about 0.5% to about 0.75% by weight water, and more preferably about 0.6% to about 0.7% by weight water. In one preferred embodiment, the temperature is maintained throughout the process at about 20*C to about 60"C and more preferably about 40*C to about 60"C. Even more preferred, the temperature is maintained at about 55*C. In another preferred embodiment, during the separation step the liquid mixture is cooled to precipitate the complex from the liquid mixture and assist in the separation of the complex from the liquid mixture. Cooling is conducted in a conventional manner using conventional equipment. Suitably, the liquid mixture is cooled a sufficient amount to precipitate the complex from the liquid mixture. More preferably, the liquid mixture is cooled to about O'C to about ICC, and even more preferably about 5 β C. Alpha-, beta-, gamma-cyclodextrin, or a mixture thereof, or modified cyclodextrin can be used in the present invention. Preferably, beta-cyclodextrin is used. The amount of cyclodextrin used in the mixture is about 5% to about 30% by weight water and more preferably about 10% to about 15% by weight water. Alternatively, the amount of cyclodextrin used is about 3 to about 5 moles per mole of cholesterol in the egg and, more preferably, about 4 moles per mole of cholesterol. The weight ratio of water to egg yolk in the mixture is about 0.7:1 to about 4:1 and more preferably about 1:1 to about 2:1. Between forming the uniform mixture and separating the complexes from the mixture it may be advantageous to employ an intermediate step of maintaining the uniformity of the mixture. Preferably the temperature of the mixture is also maintained in this intermediate step. The mixture can be maintained in its uniform condition for about 1 to about 60 minutes to allow complexation to continue to take place; more preferably for about 1 to about 20 minutes; and even more preferably for about 5 to 10 minutes. Complexation starts immediately upon mixing, however, some time may be needed to obtain a commercially reasonable reduction in cholesterol. Any food grade acid can be used to adjust the pH to about 6 to about 7 so long as it does not adversely effect the color and flavor of the egg. Suitable acids include citric, phosphoric, tartaric and acetic. Citric acid is preferred because it has no effect on the color of the egg yolk. Preferably, 0.1 molar citric acid is used to adjust the pH of the solution to about 6 to about 7 after separation of the complex from the solution. More preferably, the uniform liquid mixture is formed in a plurality of steps, those steps being: (al) forming a mixture comprising water, a food grade base, and cyclodextrin; (a2) heating said mixture to a temperature of about 20 β C to about 60°C; (a3) heating egg yolk to about 20°C to about 60°C; (a4) mixing said heated egg yolk and said heated solution to form the uniform mixture of egg yolk, water and cyclodextrin at a pH of between about 7.5 and 12 and a cyclodextrin content and water content sufficient to allow the formation of complexes between the cholesterol and the cyclodextrin. Preparing and heating the various solutions, forming the mixture and maintaining the uniformity of the mixture (optional step) are done in a conventional manner using conventional equipment. For example, a tank or tanks equipped with impellers can be used for each step of the process. Alternatively, tanks equipped with impellers can be used to heat the egg yolk and form the solution of cyclodextrin, water and food grade base, then the two are pumped together through a conduit wherein the step of forming a uniform mixture is accomplished through the union of the two solutions in a mixing means such as a Y adapter, a homogenizer, a large volume pump or a tank with an impeller, and the step of maintaining the uniformity of the mixture, if necessary, is done by means of the flow characteristics of the mixture in the conduit and with pumps in the pipes used to move the mixture through the pipes. As will be noted below, using low shear to form the mixture has produced certain advantages. Specifically, low shear helps to avoid a loss of egg yolk components from the treated egg yolk. In one embodiment, it is preferred that the mixture be formed at a low shear rate. In another embodiment, it is preferred to form the uniform mixture by vigorous stirring. When forming the uniform mixture by means of vigorous stirring, however, the mixing process should not result in the formation of a foam. Vigorous stirring causes the disassociation of the egg yolk granules and may reduce the time for formation of the complexes. This vigorous mixing is preferably accomplished using a high speed blade mixer, although other mixers can be used. The speed of blade is such that the aqueous solution is homogenized and the granules of the yolk are disassociated. The mixture is uniform when the mixture has a uniform color and is substantially uniform in chemical composition. This means that the cyclodextrin, base and water are uniformly distributed with the egg yolk. It is presumed that an emulsion forms wherein the cyclodextrin stays in the water phase and the cholesterol stays in the egg yolk phase until it complexes with the cyclodextrins. The complex remains in the water phase of the emulsion. The complex forms an insoluble precipitate which can be separated from the water and egg yolk. The emulsion formed by mixing all the components together has been found to be extremely stable. Little or no agitation is necessary to maintain the emulsion. For complexation, the emulsion must remain intact, i.e. the mixture must remain as a uniform mixture. The base can be added to the mixture any time prior to centrifugation. It has been found that it is the centrifugation which causes the loss of the protein from the egg yolk. Thus, so long as the base is added and uniformly dispensed in the mixture prior to centrifugation the protein will be maintained in the egg yolk. Addition of water to the egg yolk, in the absence of the base, causes the protein to precipitate. This precipitation is a reversible process and the base causes the protein to go back into solution if the base is added to the mixture after the protein has precipitated. It is preferred to add the base to the water prior to combining the water with the egg yolk. In other words, the water should be basic before it is added to the egg yolk to avoid the precipitation of the protein. Separation of the complexes from the mixture is done in a conventional manner using conventional equipment. A centrifuge has yielded good results. There may be residual cyclodextrin in the egg yolk after removal of the complex from solution. This residual cyclodextrin comes from unco plexed and complexed cyclodextrin which remained with the egg yolk after separation of the cyclodextrin-cholesterol complex from the yolk. Preferably, the residual cyclodextrin is removed from solution by treating the recovered egg yolk with an immobilized alpha amylase enzyme and/or a combination of alpha amylase and cyclodextrin glucosyltransferase at a temperature of about 50'C for a period of about 30 minutes in the presence of water. The weight ratio of recovered egg yolk to enzyme should be about 10:1 to about 2:1 to reduce residual cyclodextrin in said recovered egg yolk. The weight ratio of water to egg yolk should be about 0.7:1 to about 2:1; and more preferably about 1:1. The egg yolk recovered after treatment with the enzyme has been found to be substantially free of residual cyclodextrin. In order to obtain a suitable end product, the water is removed from the egg yolk in a conventional manner using conventional equipment. The moisture in the egg yolk can be reduced by ultra filtration, spray drying, or vacuum concentration. Evaporation of the egg yolk to obtain the proper solid levels has produced good results. After the complex has been separated from the egg yolk, it is preferable that the complex be further processed to break the complex and recover the cyclodextrin. The recovered cyclodextrin is subsequently recycled. To recover the cyclodextrin, the complex is suspended in water such that the weight ratio of water to complex is about 99:1 to about 4:1. The suspended complex is then agitated and heated to a temperature of about 90 β C to about 100 β C for a period of about 5 to about 30 minutes. This causes the cyclodextrin to separate from the complex; subsequently, the cyclodextrin is recovered. More preferably, the suspended complex is heated to about 95*C and the weight ratio of water to complex in the suspension is about 9:1. The recovery of the cyclodextrin is done using conventional equipment. The process of the present invention can be carried out in a batch operation, such as by using a tank equipped with an impeller into which heated egg yolk and a heated solution of water, base and cyclodextrin are added and mixed to form the uniform mixture. Alternatively, the process is carried out in a pipeline such that a heated solution of egg yolk is pumped from a first tank to join with a heated solution of water, cyclodextrin and base from a second tank. The two solutions are joined in a mixing means and then pumped through a pipe to centrifugation to separate the water and complex from the egg yolk. As shown in Figure 1, the process of the present invention can be carried out batch-wise. Tank 10 contains a solution of beta-cyclodextrin, base and water. Tank 10 is equipped with a heating means such as a water jacket, for heating the contents. Tank 10 is also equipped with impeller 12 to maintain the uniformity of the solution. Tank 14 contains egg yolks and is also equipped with a heating means such as a water jacket to heat the contents. The contents of tank 10 and tank 14 are pumped or drained into tank 16 which is equipped with an impeller 18 to form the uniform liquid mixture and to maintain the uniformity of the mixture, if necessary. Tank 16 is also equipped with a heating means such as a water jacket to maintain the temperature of the mixture. After a period of time, about 1 to about 60 minutes, the contents of tank 16 are drained through the drain cock 20 and centrifugal separating apparatus 22 separates the mixture into complex cyclodextrin paste 24 and egg yolk 26. The paste phase is then subject to a decomplexation step and recycling of the beta-cyclodextrin to tank 10 while the egg is subject to a removal of residual cyclodextrin by enzyme and evaporation step (not shown) . As shown in Figure 2, tank 30 has an impeller 32 and contains a solution of water, base and beta-cyclodextrin. Impeller 32 maintains the uniformity of the solution. Tank 30 is equipped with a heating means, such as a water jacket, to heat the contents of tank 30. Tank 34 contains egg yolk and is also equipped with a heating means such as a water jacket, to heat the contents of the tank. Pumps 36 and 38 are variable speed pumps which draw the contents of tank 30 and 34, respectively and pump them to a mixing means which for purposes of illustration is depicted as Y adapter 40. Pumps 36 and 38 are adjusted to provide the proper ratio of egg yolk to water. At Y adapter 40 the egg yolk and basic beta- cyclodextrin solution are mixed. Alternatively, in-line mixer 42 is also positioned just after Y adapter 40. The mixture then passes through pipeline 44 to centrifuge 46. The length of pipeline 44 is such that the mixture can be maintained for about 1 to about 60 minutes. Since the emulsion is so stable, there is no need for additional in-line mixers, however additional in-line mixers may be employed. Further, additional pumps may be necessary to move the mixture through the pipeline. The action of these additional pumps on the mixture provides enough additional mixing to maintain the uniformity of the mixture, if necessary. The mixture, after moving through pipeline 44 goes to centrifugal separating apparatus 46 which separates the mixture into a cyclodextrin-complex paste 48 and egg yolk 50. The paste phase is subject to a decomplexation step and a recycling of the beta-cyclodextrin to tank 30 while the egg yolk is subject to a removal of residual cyclodextrin by enzyme and an evaporation step (not shown) . As shown in Figure 3 tank 60 contains an aqueous solution of beta-cyclodextrin and base. The contents of tank 60 are stirred by paddle 62 to maintain its uniformity and heated by means of a water jacket. Tank 64 contains egg yolks which have been separated from the whites and which have been heated by means of a water jacket. Using two liquid pumps, pump 66 and pump 68 with variable speed adjustment to control flow of the liquid, the contents of each tank are pumped to a mixing means, which is depicted as Y adapter 70. Pumps 66 and 68 are adjusted to provide an appropriate water to egg yolk weight ratio and pH. At Y adapter 70 the two liquids are mixed and an emulsion formed. From Y adapter 70 the mixture passes through a short length of piping and flows into container 72. The mixture is held in container 72 for 10 minutes to 1 hour. In container 72, the temperature of the mixture is maintained with a water jacket. Eventually, the emulsion is drawn off of tank 72 and subjected to a centrifuge 76 to separate the complex 78 from the egg yolk 80. The time between forming the mixture and dumping the mixture into holding tank 72 is essentially instantaneous. The formation of complexes between the cholesterol and cyclodextrin occurs virtually instantaneously upon mixing the cyclodextrin and egg yolk together in an aqueous environment. The holding tank is used primarily to provide some flexibility in the operation. These and other aspects of the present invention may be more fully understood by reference to one or more of the following examples. EXAMPLE 1 This example illustrates the loss of egg yolk during separation of the cholesterol-cyclodextrin complex using beta-cyclodextrin without a food grade base. An aqueous solution of beta-cyclodextrin at 30% solids was prepared and then mixed with egg yolk. The water to egg yolk weight ratio was 1:1. The mixture was at ambient temperature. After about 5 to 10 minutes, a sample was collected and centrifuged at 10,000 r.p.m. for fifteen (15) minutes at 40'C. The supernatant was collected and tested for cholesterol reduction and percent weight loss. It was found that the cholesterol was reduced by 90% and that 52% by weight of the yolk was lost. Thus, although most of the cholesterol was removed from the yolk, approximately half of the egg yolk was lost in this process. EXAMPLE 2 This example illustrates the addition of sodium chloride to the process of Example 1. An aqueous suspension was prepared having a 6:3:1 weight ratio of water:beta-cyclodextrin:sodium chloride. This suspension was then mixed with egg yolk to form a mixture and maintained as such for 5 to 10 minutes. The cholesterol reduction was 83% and 45% of the yolk was lost. EXAMPLE 3 This example illustrates the present invention wherein potassium hydroxide is used to raise the pH of the system to 9.7. An aqueous suspension was prepared containing a 1:1 weight ratio of yolk:water and 12% beta-cyclodextrin by weight water. The water contained potassium hydroxide (KOH) at a level of 1.67 grams per 250 grams of water (.7%). The water and yolk were separately pre-heated to 50*C prior to mixing. The mixture was mixed at 1200 r.p.m. for 1 minute in a tank equipped with an impeller and then the mixture was passed through a pipeline equipped with a homogenizer. This pressure through the homogenizer was varied and the number of times through the homogenizer was also varied. Each mixture was centrifuged at 10,000 r.p.m. for 15 minutes at 40 * C to separate the complex from the egg yolk. The supernatant is collected as product. TABLE I Homoσenization Cholesterol Weicrht Conditions Reduction Lost Pre-homogenized 78% N.M.* 100 kg/cm2; 1st Pass 72% 15% 200 kg/cm2; 1st Pass 74% 14% 200 kg/cm2 ; 2nd Pass 76% 13% 200 kg/cm2; 3rd Pass 76% 12% 400 kg/cm2; 1st Pass 75% 13% 400 kg/cm2; 1st Pass 74% 13% *N.M. means not measured As can be seen, the cholesterol reduction was about 75% with weight loss of no more than 15%. As can be seen, the number of passes through the homogenizer and the pressure appears to have no effect on either cholesterol reduction or weight loss. EXAMPLE 4 This example illustrates the loss of egg yolk using the procedure and apparatus of Example 3, without the addition of a base. Samples were tested and the test results are reported in Table II below. TABLE II Homocfenization Cholesterol Weicfht Conditions Reduction Lost Pre-homogenized 72% 31% 1st Pass; No Pressur :ee 83% 32% As can be seen, weight loss was higher than in Example 3, but not as high as in Example 1 above. Less beta cyclodextrin is used in this example relative to Example 1 above (12% vs 30%) . Using less beta cyclodextrin gives acceptable cholesterol reduction, but still results in an unacceptable weight loss. EXAMPLE 5 This example illustrates using the process of the present invention in a batch operation using equipment similar to that shown in Figure 1. In this Example, an aqueous slurry containing 12% by weight water of beta-cyclodextrin and 0.7% KOH by weight water was mixed with egg yolk to obtain a mixture having a pH of 9.7 and a water to egg yolk weight ratio of 1:1. A number of such mixtures were prepared in a tank equipped with an impeller. Both the temperature and the rate of mixing was varied. Each mix was stirred for 5 minutes. The results of each mixture are listed below: TABLE III Cholesterol Weiαht Stir Rate Temperature °C Reduction Lost 400 r.p.m 20" 81% 22% 400 r.p.m 50" 74% 18% 800 r.p.m 20° 77% 27% 800 r.p.m 50° 75% 17% 1200 r.p.m 20° 68% 25% 1 1220000 r.p.m 50° 77% 18% As can be seen here lower mixing speeds also yield acceptable levels of cholesterol reduction. In addition it is clear that performing the experiment at an elevated temperature results in decreased weight loss. EXAMPLE 6 This example illustrates that using 10% or less of beta- cyclodextrin by weight water does not yield good results. The procedure of Example 5 was run using a 1200 r.p.m. mixing speed at 20°C. The amount of beta- cyclodextrin used in each batch as well as the weight loss of yolk and cholesterol reduction are reported in Table IV, below. TABLE IV Cholesterol BCD Amount Reduction Weiαht Lost 3% 13% 8% 5% 15% 12% 7% 15% 11% 8% 22% 10% 9% 34% 14% 10% 39% N.D.* 12% 68% 25% *N.D. means not determined As can be seen, using less than 10% cyclodextrin does not provide a commercially viable reduction in cholesterol content of the yolk. EXAMPLE 7 This example illustrates using an arrangement similar to the one shown in Figure 3 to decholesterize egg yolk. Tank 60 contained 250 ml. of a 12% by weight beta- cyclodextrin aqueous solution into which 1.67 grams of potassium hydroxide had been added. The contents of tank 60 were stirred to maintain their uniformity and heated to 55*C. Tank 64 contained egg yolks which had been separated from the whites and which had been heated to 55*C. Using two conventional liquid pumps, pump 66 and pump 68 with variable speed adjustment to control flow of the liquid, the contents of each tank were pumped to Y adapter 70. Pumps 66 and 68 were adjusted to provide a water to egg yolk weight ratio of 1:1 and a pH in the resulting mixture of about 9.7. At Y adapter 70 the two liquids were mixed and the mixture formed. From Y adapter 70 the mixture passed through approximately six inches of 0.75 inch (ID) piping at a flow rate of 150 ml. per minute and into a container. After the mixture was formed it was held in container 72 for 5 to 10 minutes and then subjected to a centrifuge 76 to separate the complex from the egg yolk. No in-line mixer or pipe line was used. The resulting egg yolk was then tested for both weight loss and cholesterol reduction. The weight loss was 13% and the cholesterol reduction was 75%. EXAMPLE 8 This example illustrates that increasing the mixing time does not increase the cholesterol reduction or decrease the weight loss and that formation of the complexes is virtually instantaneous. The apparatus in accordance with Figure 2 was employed along with an in-line mixer 42. Tank 30 contained 250 ml. of a 12% by weight beta-cyclodextrin aqueous solution into which 1.67 grams of potassium hydroxide had been added. The contents of tank 30 were stirred to maintain their uniformity and heated to 55"C. Tank 34 contained egg yolks which had been separated from the whites and which had been heated to 55°C. Using two conventional liquid pumps, pump 36 and pump 38 with variable speed adjustment to control flow of the liquid, the contents of each tank were pumped to Y adapter 40. Pumps 36 and 38 were adjusted to provide a water to egg yolk weight ration of 1:1 and a pH in the resulting mixture of about 9.7. At Y adapter 40 the two liquids were mixed and the mixture formed. From Y adapter 40 the mixture passed through approximately six inches of 0.75 inch (ID) piping at a flow rate of 150 ml. per minute and into in-line mixer 42. In-line mixer 42 had a length of 11 inches and internal diameter of 7/16 inch. The total time for both mixing and collecting was approximately 10 seconds. The mixture was collected and centrifuged as in Example 7. No pipeline 42 was used. The resulting egg yolk had a weight loss of 12% and cholesterol reduction of 75%. EXAMPLE 9 This example illustrates using both high shear to form the uniform mixture of egg yolk, water and cyclodextrin as well as the optional cooling step for aiding in the precipitation of the complex during the separation step. Liquid egg yolks obtained from chicken had their pH adjusted to 9 with 1 N KOH. Next, water was added in an amount to provide a 3:1 w/w water to solid ratio. Then the liquids were heated to 50"C and beta-cyclodextrin added in an amount to provide a mole ratio of 4:1, cyclodextrin to cholesterol. Finally, a uniform mixture was formed without foaming by mixing the liquids and cyclodextrin for 10.5 minutes at 1600 rpm in an omnimixer homogenizer (Model 17105) . The temperature remained at 50°C throughout the mixing. After mixing, the liquids were cooled to 8-10*C within one hour to stabilize the complexes and recrystallize the free cyclodextrin in solution. The insoluble complexes and recrystallized free cyclodextrin was then separated from the cooled mixture by centrifugation. The centrifuge was operated at a force of 1080 x g for 10 minutes. The mixture had a temperature of 5 β C during centrifugation. Although the cooler temperature of the mixture increased the amount of energy necessary for centrifugation, when compared to the mixture at 50"C, the cooler temperature slows microbiol growth and aids in reducing residual cyclodextrin. After the centrifugation, it was found that cholesterol was reduced by 83% and only a 14% loss of solids. EXAMPLE 10 This example illustrates using the process as described in Example 9 above, except that egg white rather than water is used to dilute the egg yolk. Following the steps in example 9 above, egg white was added to the egg yolk after the addition of KOH to provide a 2.9:1 w/w water to solid ratio. The advantage to using egg white rather than water is economics. Otherwise all the steps recited in Example 9 were followed. After centrifugation, the supernatant, the egg yolk with reduced cholesterol, had its pH adjusted with 1 N HC1 to its native value to reassociate the granules and lipoproteins. The egg yolk had its cholesterol reduced by 80%. EXAMPLE 11 Both conventional egg yolk, without reduced cholesterol and egg yolk with reduced cholesterol made in accordance with the present invention were tested in a foodstuff. Specifically, baking tests were performed to make a sponge cake. There is a positive correlation between sponge cake volume and protein solubility, the higher the protein solubility the greater the sponge cake volume. It was found that there was no significant difference between the sponge cake volume made with the reduced cholesterol egg yolks of the present invention and the conventional egg yolks. It was also found that the visual and texture of the sponge cake made with the reduced cholesterol egg yolk of the present invention and the sponge cake made with conventional egg yolk was similar. Thus, the function of the reduced cholesterol egg yolk of the present invention appears to be virtually identical to that of a conventional egg yolk without reduced cholesterol. It will be understood that the claims are intended to cover all changes and modifications of the preferred embodiments of the invention herein chosen for the purpose of illustration which do not constitute a departure from the spirit and scope of the invention.

Claims: What is claimed is: 1. A process for reducing cholesterol from egg yolk comprising the steps of: (a) forming a uniform liquid mixture comprising water, food grade base, cyclodextrin and egg yolk, at a temperature of about 20 β C to about 60"C, said base being present in an amount sufficient to adjust the pH of said mixture to between about 7.5 and 12, said cyclodextrin and said water being present in an amount sufficient to form complexes form between the cholesterol and said cyclodextrin; (b) separating said complexes from said mixture; (c) adjusting the pH of said solution to about 6 to about 7 by means of a food grade acid; and (d) recovering an egg yolk with reduced cholesterol. 2. The process of claim 1 wherein the food grade base is selected from the group consisting of sodium hydroxide, potassium hydroxide, sodium carbonate or potassium carbonate. 3. The process of claim 1 wherein the amount of cyclodextrin used in said mixture is about 5% to about 30% by weight water in said mixture. 4. The process of claim 1 wherein the amount of cyclodextrin used in said mixture is about 3 moles to about 5 moles per mole of cholesterol in said egg yolk. 5. The process of claim 1 wherein said mixture has a water to egg yolk ratio of about 0.7:1 to about 4:1. 6. The process of claim 1 further comprising the step of maintaining said uniform mixture in a uniform state for a period of about 1 to about 60 minutes prior to separating said complexes from said mixture. 7. The process of claim 1 wherein the food grade acid is selected from the group consisting of citric acid, phosphoric acid, tartaric acid and acetic acid. 8. The process of claim 1 further comprising the step of cooling the uniform liquid mixture to precipitate the complexes from the mixture prior to separating said complexes from said mixture. 9. The process of claim 1 further comprising the steps of treating said recovered egg yolk with an immobilized alpha amylase enzyme and/or a combination of alpha amylase and cyclodextrin glucosyltransferase at a temperature of about 50'C for a period of about 30 minutes to remove residual cyclodextrin from the egg yolk. 10. The process of claim 1 wherein said uniform mixture has a temperature of about 40°C to about 50°C. 11. The process of claim 1 wherein said separating step is conducted by centrifugation. 12. The process of claim 1 wherein said step of forming a uniform liquid mixture comprises the steps of: (al) forming a mixture of water, a food grade base and cyclodextrin wherein the cyclodextrin is present in an amount of about 5% to about 30% by weight water and said food grade base is present in an amount to give the mixture a pH of about 7.5 to 12. (a2) heating said mixture to a temperature of about 40'C to about 60°C. (a3) heating egg yolk to a temperature of about 40°C to about 60°C and (a4) combining said heated egg yolk and said heated mixture to form said uniform mixture of said water, egg yolk, cyclodextrin and food grade base. 13. The process of claim 12 further comprising the step of maintaining said uniform mixture in a uniform state for a period of about 1 to about 60 minutes prior to separating said complexes from said mixture. 14. The process of claim 12 further comprising the step of cooling the uniform liquid mixture to precipitate the complexes from the mixture.

Inventors: AWAD AZIZ, HEDGES ALLAN, SHIEH WEN, SIKORSKI CHRISTOPHER, SMITH DENISE M, AWAD, AZIZ, HEDGES, ALLAN, SHIEH, WEN, SIKORSKI, CHRISTOPHER, SMITH, DENISE, M.
Applicants: UNIV MICHIGAN STATE, BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIVERS
Application year: 1996
Main classification: A23L1
IPC codes: A23L1