PIPERAZINE, PIPERIDINE AND TETRAHYDROPYRIDINE DERIVATIVES

Abstract: A class of N-substituted piperazine, piperidine and tetrahydropyridine derivatives, linked by a fluoro-substituted alkylene chain to a fused bicyclic heteroaromatic moiety such as indolyl, and further substituted at the 4-position by an optionally substituted alkenyl, alkynyl, aryl-alkyl or heteroaryl-alkyl moiety, are selective agonists of 5-HT1-like receptors, being potent agonists of the human 5-HT1D alpha receptor subtype whilst possessing at least a 10-fold selective affinity for the 5-HT1D alpha receptor subtype relative to the 5-HT1D beta subtype; they are therefore useful in the treatment and/or prevention of clinical conditions, in particular migraine and associated disorders, for which a subtype-selective agonist of 5-HT1D receptors is indicated, whilst eliciting fewer side-effects, notably adverse cardiovascular events, than those associated with non-subtype-selective 5-HT1D recptor agonists.

Description: PIPERAZINE. PIPERIDINE AND TETRAHYDROPYRIDINE DERIVATIVES The present invention relates to a class of substituted piperazine, piperidine and tetrahydropyridine derivatives which act on 5 -hydroxy tryptamine (5-HT) receptors, being selective agonists of so-called "5-HTι-like" receptors. They are therefore useful in the treatment of clinical conditions for which a selective agonist of these receptors is indicated. It has been known for some time that 5-HTι-like receptor agonists which exhibit selective vasoconstrictor activity are of use in the treatment of migraine (see, for example, A. Doenicke et al., Uie Lancet, 1988, Vol. 1, 1309-11; and W. Feniuk and P.PA. Humphrey, Drug Development Research, 1992, 26, 235-240). The human 5-HTι-like or 5-HTID receptor has recently been shown by molecular cloning techniques to exist in two distinct subtypes. These subtypes have been termed 5-HTID Q (or 5-HTID-I) and 5-HTiD β (or 5-HTID-2), and their amino acid sequences are disclosed and claimed in WO-A-91/17174. The 5-HTiD α receptor subtype in humans is believed to reside on sensory terminals in the dura mater. Stimulation of the 5-HTiD α subtype inhibits the release of inflammatory neuropeptides which are thought to contribute to the headache pain of migraine. The human 5-HTiD p receptor subtype, meanwhile, is located predominantly on the blood vessels and in the brain, and hence may play a part in mediating constriction of cerebral and coronary arteries, as well as CNS effects. Administration of the prototypical 5-HTm agonist sumatriptan (GR43175) to humans is known to give rise at therapeutic doses to certam adverse cardiovascular events (see, for example, F. Willett et al., Br. Med J., 1992, 304, 1415; J.P. Ottervanger et ai, The Lancet, 1993, 341, 861-2, and D.N. Bateman, The Lancet, 1993, 341, 221-4) Since sumatriptan barely discriminates between the human 5-HTiD α and 5-HTiDρ receptor subtypes (cf. WO-A-91/17174, Table 1), and since it is the blood vessels with which the 5-HTiD β subtype is most closely associated, it is believed that the cardiovascular side-effects observed with sumatriptan can be attributed to stimulation of the 5-HTiDp receptor subtype. It is accordingly considered (cf. G.W. Rebeck et al, Proc. Natl. Acad. Sci. USA, 1994, 91, 3666-9) that compounds which can interact selectively with the 5-HTi Dα receptor subtype, whilst having a less pronounced action at the 5-HT IDP subtype, might be free from, or at any rate less prone to, the undesirable cardiovascular and other side-effects associated with non-subtype-selective 5-HT receptor agonists, whilst at the same time maintaining a beneficial level of anti-migrame activity. The compounds of the present invention, being selective 5-HTι-like receptor agonists, are accordingly of benefit in the treatment of migraine and associated conditions, e.g. cluster headache, chronic paroxysmal hemicrama, headache associated with vascular disorders, tension headache and paediatπc migraine. In particular, the compounds according to this invention are potent agonists of the human 5-HTιo α receptor subtype. Moreover, the compounds m accordance with this invention have been found to possess at least a 10-fold selective affinity for the 5-HTiϋ α receptor subtype relative to the 5-HTiDp subtype, and thej' can therefore be expected to manifest fewer side-effects than those associated with non-subtype-selective 5-HTID receptor agonists Several distinct classes of substituted five-membered heteroaromatic compounds are described in published European patent applications 0438230, 0494774 and 0497512, and published International patent applications 93/18029, 94/02477 and 94/03446 The compounds described therein are stated to be agonists of 5-HTι-lιke receptors, and accordingly to be of particular use in the treatment of migraine and associated conditions None of these publications however, discloses nor even suggests the piperazine, piperidine and tetrahydropyndine derivatives provided by the present invention. In EP-A-0548813 is described a series of alkoxypyridιn-4-yl and alkoxypyrιmιdin-4-yl derivatives of indol-3-ylalkylpiperazmes which are alleged to provide treatment of vascular or vascular-related headaches, including migraine. There is, however, no disclosure nor any suggestion in EP-A-0548813 of replacing the alkoxypyridine or alkoxypyπmidine substituent with an optionally substituted alkenyl, alkynyl, aryl-alkyl or heteroaryl-alkyl substituent; nor is there any suggestion therein that the alkylene chain present in the ιndol-3-ylalkylpiperazine portion of the molecule might be substituted with one or more fluorine atoms. WO-A-91/18897 describes a class of tryptamine derivatives substituted by various five-membered rings, which are stated to be specific to a particular type of "5-HTι-like" receptor and thus to be effective agents for the treatment of clinical conditions, particularly migraine, requiring this activity A further class of tryptamine derivatives with alleged anti- migraine activity is disclosed in WO-A-94/02460. However, neither WO-A-91/18897 nor WO-A-94/02460 discloses or suggests the piperazine, piperidme and tetrahydropyndine derivatives provided by the present invention. Moreover, nowhere m the prior art mentioned above is there any disclosure of a subtype-selective 5-HTID receptor agonist having a 5-HTiD α receptor binding affinity (IC50) below 50 nM and at least a 10-fold selective affinity for the 5-HTιo α receptor subtype relative to the 5-HTID P subtype. The compounds according to the present invention are subtype- selective 5-HTID receptor agonists having a human 5-HTiD α receptor binding affinity (IC50) below 50 nM, typically below 10 nM and preferably below 1 nM; and at least a 10-fold selective affinity, typically at least a 50- fold selective affinity and preferably at least a 100-fold selective affinity, for the human 5-HTm ra receptor subtype relative to the 5-HTiDp subtype Moreover, the compounds in accordance with this invention possess interesting properties in terms of their efficacy and/or bioavailabiUty. The present invention provides a compound of formula I, or a salt or prodrug thereof: wherein Z represents hydrogen, halogen, cyano, nitro, trifluoromethyl, -OR 5 , -OCOR 5 , -OCONR 5 R 6 , -OCH 2 CN, -OCH 2 CONR 5 R 6 , -SR 5 , -SOR 5 , -SO 2 R 5 , -SO 2 NR 5 R G , -NR 5 R G , -NR 5 COR G , -NR 5 CO 2 R 6 , -NR S S0 2 R 6 , -COR 5 , -CO 2 R 5 , -CONR 5 R 6 , or a group of formula (Za), (Zb), (Zc) or (Zd): (Za) (Zb) (Zc) (Zd) in which the asterisk * denotes a chiral centre; or Z represents an optionally substituted five-membered heteroaromatic ring selected from furan, thiophene, pyrrole, oxazole, thiazole, isoxazole, isothiazole, imidazole, pyrazole, oxadiazole, thiadiazole, triazole and tetrazole; X represents oxygen, sulphur, -NH- or methylene; Y represents oxygen or sulphur; E represents a chemical bond or a straight or branched alkylene chain containing from 1 to 4 carbon atoms; Q represents a straight or branched alkylene chain containing from 1 to 6 carbon atoms, substituted in any position by one or more fluorine atoms; T represents nitrogen or CH; U represents nitrogen or C-R 2 ; V represents oxygen, sulphur or N-R 3 ; -F-G- represents -CH2-N-, -CH2-CH- or -CH=C-; R 1 represents C36 alkenyl, C3-6 alkynyl, aryl(Cι 6)alkyl or heteroaryl(Cι G)alkyl, any of which groups may be optionally substituted; R 2 , R 3 and R 4 independently represent hydrogen or Ci G alkyl, and R 5 and R 6 independently represent hydrogen, Ci 6 alkyl, trifluoromethyl, phenyl, methylphenyl, or an optionally substituted aryl(Cι-6)alkyl or heteroaryl(Cι-6)alkyl group; or R 5 and R 6 , when linked through a nitrogen atom, together represent the residue of an optionally substituted azetidme, pyrrolidine, piperidine, morpholine or piperazine Where Z in the compounds of formula I above represents a five- membered heteroaromatic ring, this ring may be optionally substituted by one or, where possible, two substituents. As will be appreciated, where Z represents an oxadiazole, thiadiazole or tetrazole ring, only one substituent will be possible; otherwise, one or two optional substituents may be accommodated around the five-membered heteroaromatic ring Z Examples of suitable substituents on the five-membered heteroaromatic ring Z include Ci 6 alkyl, C26 alkenyl, C 2 6 alkynyl Cs 7 cycloalkyl, aryl, aryl(Cj. c)alkyl, C 3 7 heterocycloalkyl, heteroaryl, heteroaryl(Cι r,)alkyl, Ci r > alkoxy, Ci 6 alkylthio, amino, Ci G alkylamino, dι(Cι G)alkylammo, halogen cyano and trifluoromethyl. The group R 1 may be optionally substituted by one or more substituents, as also mav the groups R r> or R 6 where these represent aryl(Cι-6) alkyl or heteroaryl(Cι-6)alkyl. Where R 1 , R 5 or R 6 represents aryl(C 1 .6)alkyl or heteroaryl(Cι-6)alkyl, any optional substitution will suitably be on the aryl or heteroaryl moiety thereof, although substitution on the alkyl moiety thereof is an alternative possibility. Examples of optional substituents thereon mclude halogen, cyano, trifluoro methyl, triazolyl, tetrazolyl, Cι-6 alkyl-tetrazolyl, hydroxy, Cι-6 alkoxy, Cι- 6 alkylthio, C26 alkoxycarbonyl, C2-6 alkylcarbonyl, d-6 alkylsulphonyl, arylsulphonyl, ammo, CI-G alkylamino, dι(Cι-G) alkylamino, dι(Cι-G)alkylamιnomethyl, C2-6 alkylcarbonylam o, arylcarbonylamino, C 2 6 alkoxycarbonylamino, N-(Cι 6)alkyl-N-(C26)alkoxycarbonylammo, Ci G alkylsulphonylamino, arylsulphonylammo, Ci G alkylsulphonylaminomethyl, aminocarbonylamino, C1-6 alkylaminocarbonylammo, di(Cι 6)alkylamιnocarbonylamιno, mono- or diarylammocarbony lam o, pyrrolid y lcarbonylammo , piperidinylcarbonylammo, aminocarbonyl, CI-G alkylaminocarbonyl, dι(Cι-G)alkylammocarbonyl, ammosulphonyl, Cι-6 alkylammosulphonyl, di(Cι-6)alkylamιnosulphonyl, aminosulphonylmethyl, d-G alkylammosulphonylmethyl and dι(Cι 6)alkylamιnosulphonylmethyl. When R 5 and R G , when linked through a nitrogen atom, together represent the residue of an azetidine, pyrrohdine, piperidme, morphohne or piperazine ring, this ring may be unsubstituted or substituted by one or more substituents. Examples of suitable substituents include Ci 6 alkyl, aryl(d.G)alkyl, Ci G alkoxy, C 2 G alkoxycarbonyl and Ci G alkylaminocarbonyl. Typical substituents include methyl, benzyl, methoxy, methoxycarbonyl, ethoxycarbonyl and methylammocarbonyl In particular, where R" 5 and R G together represent the residue of a piperazine ring, this ring is preferably substituted on the distal nitrogen atom by a C 2 G alkoxycarbonyl moiety such as methoxycarbonyl or ethoxycarbonyl As used herein, the expression "Ci G alkyl" includes methyl and ethyl groups, and straight-chained or branched propyl, butyl, pentyl and hexyl groups Particular alkyl groups are methvl, ethyl, ropropyl, isopropyl and tert-butyl. Derived expressions such as "Cι-6 alkoxy", "Ci-β alkylthio" and "Ci-β alkylamino" are to be construed accordingly. The expression "C2-6 alkenyl" as used herein refers to straight- chained and branched alkenyl groups containing from 2 to 6 carbon atoms. Typical examples include vmyl, allyl, dimethylallyl and butenyl groups. The expression "C2-6 alkynyl" as used herem refers to straight- chained and branched alkynyl groups containmg from 2 to 6 carbon atoms. Typical examples include ethynyl and propargyl groups. Typical C3 1 cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. Typical aryl groups include phenyl and naphthyl. The expression "aryl(Cι-6)alkyl" as used herein includes benzyl, phenylethyl, phenylpropyl and nap hthy lme thyl. Suitable heterocycloalkyl groups mclude azetidinyl, pyrrolidinyl, pipeπdmyl, piperazinyl and morpholmyl groups. Suitable heteroaryl groups include pyridinyl, quinohnyl, isoqumolmyl, pyridazinyl, pyrimidinyl, pyrazinyl, pyranyl, furyl, benzofuryl, dibenzo furyl, thienyl, benzthienyl, pyrrolyl, indolyl, pyrazolyl, mdazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, benzimidazolyl, oxadiazolyl, thiadiazolyl, triazolyl and tetrazolyl groups The expression "heteroaryl(Cι G)alkyl" as used herein includes furylmethyl, furylethyl, thienylmethyl, thienylethyl, oxazolylmethyl, oxazolylethyl, thiazolylmethyl, thiazolylethyl, imidazolylmethyl, lmidazolylethyl, oxadiazolylmethyl, oxadiazolylethyl, thiadiazolylmethyl, thiadiazolylethyl, tπazolylmethyl, tπazolylethyl, tetrazoly lme thyl, tetrazolylethyl, pyπdmylmethyl, pyridinylethyl, pyπdmylpropyl, pyπmidinylmethyl, pyrazinylmethyl, qumolmylmethyl and isoq umoliny Imethyl The term "halogen" as used herein includes fluorine, chlorine bromine and iodine, especially fluorine. For use in medicine, the salts of the compounds of formula I will be pharmaceutically acceptable salts. Other salts may, however, be useful m the preparation of the compounds according to the invention or of their pharmaceutically acceptable salts Suitable pharmaceutically acceptable salts of the compounds of this invention mclude acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, oxalic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts, e.g. sodium or potassium salts; alkaline earth metal salts, e.g. calcium or magnesium salts; and salts formed with suitable organic ligands, e.g. quaternary ammonium salts. The present invention includes within its scope prodrugs of the compounds of formula I above. In general, such prodrugs will be functional derivatives of the compounds of formula I which are readily convertible in υwo into the required compound of formula I Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in Design of Prodrugs, ed. H Bundgaard, Elsevier, 1985 Where the compounds according to the invention have at least one asymmetric centre, they may accordingly exist as enantiomers Where the compounds according to the invention possess two or more asymmetric centres, they may additionally exist as diastereoisomers For example, the compounds of formula I above wherein Z represents a group of formula (Zb) or (Zc) have a chiral centre denoted by the asterisk *, which may accordmgly be in the (R) or (S) configuration It is to be understood that all such isomers and mixtures thereof in any proportion are encompassed within the scope of the present invention The alkylene chain E may be, for example, methylene, ethylene, 1- methylethylene, propylene, 2-methylpropylene or butylene. In addition, E may represent a chemical bond such that the moiety Z is attached directly to the central fused bicyclic heteroaromatic ring system containing the variables T, U and V. Suitably, E represents a chemical bond or a methylene linkage. The alkylene chain Q is substituted in any position by one or more fluorine atoms, preferably by one or two fluorine atoms and more preferably by one fluorine atom. Representative alkylene chains for Q include 2-fluoropropylene, 2,2-difluoropropylene and 2-fluoromethyl- propylene, especially 2-fluoropropylene or 2-fluoromethyl-propylene. The compound of formula I in accordance with the present invention is suitably an indole, benzofuran or benzthiophene derivative of formula IA, an indazole derivative of formula IB, or a pyrrolo [2, 3-c]pyridine derivative of formula IC: wherem Z, E, Q, V, F, G, R 1 , R 2 and R 3 are as defined above. Preferably, the compounds according to the invention are indole or pyrrolo[2,3-c]- pyridine derivatives of formula ID: wherein Z, E, Q, T, F, G, R 1 , R 2 and R 3 are as defined above, in particular wherein R 2 and R 3 are both hydrogen. Suitable values for the substituent R 1 include allyl, dimethylallyl, butenyl, propargyl, benzyl, phenylethyl, phenylpropyl, furylmethyl, thienylmethyl, thienylethyl, imidazolylmethyl, pyridinylmethyl and pyridinylpropyl, any of which groups may be optionally substituted. Selected values of R 1 include allyl, dimethylallyl, butenyl, propargyl, benzyl, phenylethyl, phenylpropyl, furylmethyl, thienylmethyl, imidazolylmethyl and pyridinylmethyl, any of which groups may be optionally substituted Typical substituents on the group R 1 include halogen, cyano, trifluoromethyl, triazolyl, tetrazolyl, Ci G alkyl-tetrazolyl, Ci G alkoxy, ammo, dι(Cι 6)alkylamιno, dι(Cι 6)alkylamιnomethyl, C 2 ι > alkylcarbonylammo, C 2 6 alkoxycarbonylamino, N-(C] G)alkyl-N- (C26)alkoxycarbonylammo, CI-G alkylsulphonylamino, ammocarbonylamino, aminocarbonyl, Ci <, alkylaminocarbonyl, dι(Cι G)alkylammocarbonyl, aminosulphonyl and Ci G alkylaminosulphonylmethyl. Representative values of R 1 include allyl dimethylallyl, butenyl, propargyl, benzyl, fluorobenzyl, difluorobenzyl, cyanobenzyl, tetrazolyl- benzyl, methyltetrazolyl-benzyl, methoxybenzyl, aminobenzyl, dimethylaminomethyl-benzyl, acetylammo-benzyl, aminocarbonvl-benzyl, methylammocarbonyl-benzyl, dimethylammocarbonyl-benzvl, aminosulphonyl-benzyl, phenylethyl, fluoro-phenylethyl, difluoro- phenylethyl, trifluoromethyl-phenylethyl, cyano-phenylethyl, triazolyl- phenylethyl, amino-phenylethyl, dimethylamino-phenylethyl, acetylamino-phenylethyl, methoxycarbonylamino-phenylethyl, (N-methyl- N-methoxycarbonyl)amino-phenylethyl, aminocarbonylamino-phenylethyl, phenylpropyl, fluoro-phenylpropyl, furylmethyl, thienylmethyl, thienylethyl, imidazolylmethyl, pyridinylmethyl, pyridinylpropyl and amino-pyridylmethyl. Particular values of R 1 include benzyl, phenylethyl, fluoro- phenylethyl, phenylpropyl (especially 2 -phenylpropyl), fluoro-phenylpropyl (especially 2 -(fluorophenyl) -propyl), thienylethyl, pyridinylmethyl and pyridinylpropyl (especially 2-(pyridinyl)-propyl). Suitably, R 2 and R 3 independently represent hydrogen or methyl, especially hydrogen. Suitably, R 4 represents hydrogen or methyl, especially hydrogen. Suitably, R 5 and R 6 are independently selected from hydrogen, methyl, ethyl, n-propyl, isopropyl, n-butyl, tert-butyl, trifluoromethyl, phenyl, methylphenyl (especially 4-methylphenyl), benzyl and phenethyl. Suitably, the substituent Z represents hydrogen, fluoro, cyano, hydroxy, methoxy, ethoxy, benzyloxy, methylamino-carbonyloxy, cyano- methoxy, aminocarbonyl-methoxy, methylsulphonyl, aminosulphonyl, N- methylamino-sulphonyl, N,N-dimethylamino-sulphonyl, amino, formylamino, acetylamino, trifluoromethyl-carbonylamino, benzyloxy- carbonylamino, methyl-sulphonylamino, ethyl-sulphonylamino, methylphenyl-sulphonylamino, N-methyl-(N-methylsulphonyl)-amino, N-methyl-(N-ethylsulphonyl)-amino, N-methyl-(N- trifluoromethylsulphonyl)-amino, N-ethyl-(N-methylsulphonyl)-amino, N- benzyl-(N-methylsulphonyl)-amino, N-benzyl-(N-ethylsulphonyl)-ammo, acetyl, methoxycarbonyl, ethoxycarbonyl, aminocarbonyl, methylaminocarbonyl, ethylaminocarbonyl, propylaminocarbonyl, butylaminocarbonyl, benzylammocarbonyl or phenethyl-aminocarbonvl; or a group of formula (Za), (Zb), (Zc) or (Zd) as defined above, or an optionally substituted five-membered heteroaromatic ring as specified above. In a particular embodiment, Z represents -SO2NR 5 R G in which R 5 and R G are as defined above. In a subset of this embodiment, R 5 and R G independently represent hydrogen or Cι-6 alkyl, especially hydrogen or methyl. Particular values of Z in this context mclude aminosulphonyl, N- methylamino-sulphonyl and N,N-dιmethylamιno-sulphonyl, especially N- methylamino-sulphonyl. In another embodiment, Z represents a group of formula (Zb) in which R 4 is hydrogen or methyl. In a subset of this embodiment, X and Y both represent oxygen. In a particular aspect of this subset, the chiral centre denoted by the asterisk * is in the (S) configuration When the group Z represents an optionally substituted five- membered heteroaromatic ring, this is suitably a 1,3-oxazole, 1,3-thιazole, imidazole, 1,2,4-oxadιazole, 1,3,4-oxadιazole, 1,2,4-thιadιazole, 1,3,4- thiadiazole, 1,2,3-trιazole, 1,2,4-trιazole or tetrazole ring. Preferably, the ring is a 1,3-oxazole, 1,3-thιazole, 1,2,4-oxadιazole, 1,2,4-thιadιazole or 1,2,4-tnazole ring, in particular a 1,2,4-trιazol-l-yl or l,2,4-trιazol-4-yl moiety Suitably, the five-membered heteroaromatic ring Z is unsubstituted Examples of optional substituents which may typically be attached to the moiety Z include methyl, ethyl, benzyl and amino A particular sub-class of compounds accordmg to the invention is represented by the compounds of formula IIA. and salts and prodrugs thereof wherein m is zero, 1, 2 or 3, preferably zero or 1; p is zero, 1 or 2; Q 1 represents a straight or branched alkylene chain containing from 2 to 5 carbon atoms, substituted in any position by one or more fluorine atoms; T represents nitrogen or CH; G 1 represents nitrogen or CH; A represents nitrogen or CH; B represents nitrogen or C-R 8 ; R 7 and R 8 independently represent hydrogen, Ci β alkyl, C 2 G alkenyl, C 3 i cycloalkyl, aryl, aryl(Cι 6)alkyl, C 3 -7 heterocycloalkyl, heteroaryl, heteroaryl(Cι-G)alkyl, Ci-β alkoxy, Ci G alkylthio, ammo, Ci r, alkylamino, dι(Cι G)alkylammo, halogen, cyano or trifluoromethyl, W represents a group of formula (Wa), (Wb) or (Wc) (Wa) (Wb) (Wc) in which W 1 represents CH or nitrogen, W 2 represents oxygen, sulphur, NH or N-methyl; R 9 represents hydrogen, halogen, cyano, trifluoromethyl, triazolyl, tetrazolyl, Cι-6 alkyl-tetrazolyl, Cj-6 alkoxy, C2-G alkylcarbonyl, ammo, C I - G alkylamino, di(Cι-6)alkylamιno, di(Cι-6)alkylamιnomethyl, C 2 -6 alkylcarbonylamino, Cι-6 alkylsulphonylamino, aminocarbonylamino, - 6 alkylaminocarbonyl, aminosulphonyl or CI-G alkylaminosulphonylmethyl; and R 10 represents hydrogen or C1-3 alkyl. Suitably, Q 1 represents a straight or branched 3 or 4 carbon alkylene chain, substituted in any position by one or two fluorine atoms Where the alkylene linkage Q 1 is substituted by two fluorine atoms, the gem difluoro substitution pattern is preferred. Particular alkylene chains for Q 1 include 2-fluoropropylene, 2,2-difluoropropylene and 2- (fluoromethyl)-propylene, especially 2-fluoropropylene or 2-(fluoromethyl)- propylene. Particular values of R 7 and R 8 include hydrogen, methyl, ethyl, benzyl and amino, especially hydrogen. Particular values of R 9 include hydrogen, fluoro, cyano, triazolyl, tetrazolyl, methyl-tetrazolyl, methoxy, amino, dimethylammomethyl, acetylamino, aminocarbonylamino, methylaminocarbonyl and aminosulphonyl, especially hydrogen or fluoro Particular values of R 10 include hydrogen and methyl. Another sub-class of compounds according to the invention is represented by the compounds of formula IIB, and salts and prodrugs thereof (HB) wherein m, p, Q 1 , T, G 1 , W and R 10 are as defined with reference to formula HA above; and R 5 and R G are as defined with reference to formula I above. Particular values of R 5 and R G in relation to formula IIB above mclude hydrogen and Cι-6 alkyl, especiaUy hydrogen or methyl. Suitably, one of R 5 and R 6 represents hydrogen and the other represents hydrogen or methyl. A further sub-class of compounds according to the invention is represented by the compounds of formula IIC, and salts and prodrugs thereof: (HC) wherein the asterisk * denotes a chiral centre; m, p, Q 1 , T, G 1 , W and R 10 are as defined with reference to formula IIA above; and R 4 and Y are as defined with reference to formula I above. Particular values of R 4 in relation to formula IIC mclude hydrogen and methyl. Preferably, Y in formula IIC is oxygen Preferably, the chiral centre denoted by the asterisk * m formula IIC is m the (S) configuration. Specific compounds within the scope of the present invention mclude: 4-benzyl-l-[2-fluoromethyl-3-(5-(l,2,4-tnazol-4-yl)-lH-mdol-3- yl)propy l]pιperazme ; 4-[2-(3-fluorophenyl)ethyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-ιndol-3- yl)propyl]pιperazme; 4-benzyl-l-[2-fluoro-3-(5-(l,2,4-tnazol-4-yl)-lH-mdol-3- yl)propyl]pιperιdιne; 4-[2-(2-fluorophenyl)propyl]-l-[2-fluoro-3-(5-(l,2,4-tnazol-4-yl)-lH-mdol-3- yl)propyl]pιperazme; 4-[2-(3-fluorophenyl)propyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]pιperazme; 4-[2-(4-fluorophenyl)propyl}-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)- lH-mdol-3- y l)propyl]pιperazιne ; 4-(2-phenylethyl)-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-ιndol-3- yl)propyl]pιperazιne, 4-(2-phenylpropyl)-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol-3- y l)propy l]pιper az e , 4-[2-(2-fluorophenyl)ethyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lΗ-ιndol-3- yl)propyl]pιperazιne, 4-[2-(4-fluorophenyl)ethyl]-l-[2-fluoro-3-(5-(l,2 4-trιazol-4-yl)-lH-mdol-3- yl)propyl]pιperazme, 4-[2-(3-pyπdyl)propyl]-l-[2-fluoro-3-(5-(l 2,4-tnazol-4-vl)-lH-mdol-3- yl)propyl]pιperazme, 4-[2-(4-pyrιdyl)propyl]-l-[2-fluoro-3-(5 (l,2,4-tπazol-4-vD-lH-ιndol-3- yl)propyl]pιperazιne, 4-[2-(2-pyrιdyl)propyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]pιperazιne ; 4-[2-(3-fluorophenyl)ethyl]-l-[(S)-2-fluoro-3-(5-(l,2,4-tnazol-4-yl)-lH-ιndol- 3-yl)propyl]pιperazιne ; 4-[2-(3-fluorophenyl)ethyl]-l-[(R)-2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-ιndol- 3-yl)propyl]pιperazιne; 4-[2-(3-fluorophenyl)ethyl]-l-[2,2-difluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-ιndol- 3-yl)propyl]pιperazme; 4-(2-phenylpropyl)-l-[2,2-difluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-ιndol-3- yl)propyl]pιperazme; 4-[2-(4-fluorophenyl)propyl]-l-[2,2-difluoro-3-(5-(l,2,4-tnazol-4-yl)-lH- ιndol-3-yl)propyl]pιperazme; 4-[2-(2-thιenyl)ethyl]-l-[2,2-difluoro-3-(5-(l,2,4-tπazol-4-yl)-lH-mdol-3- yl)propyl]piperazine; 4-[2-(3-thιenyl)ethyl]-l-[2,2-difluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]piperazme; 4-[(S)-2-phenylpropyl]-l-[2,2-difluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]pιperazιne; 4-[2-(3-pyrιdyl)propyl]-l-[2,2-dιfluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]pιperazιne; and salts and prodrugs thereof. The invention also provides pharmaceutical compositions comprising one or more compounds of this invention m association with a pharmaceutically acceptable carrier. Preferably these compositions are in unit dosage forms such as tablets, pills, capsules, powders, granules, sterile parenteral solutions or suspensions, metered aerosol or liquid sprays, drops, ampoules, auto-injector devices or suppositories; for oral, parenteral, intranasal, sublingual or rectal administration, or for administration by inhalation or insufflation For preparing solid compositions such as tablets, the principal active ingredient is mixed with a pharmaceutical carrier, e.g conventional tableting ingredients such as corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums, and other pharmaceutical diluents, e.g. water, to form a sohd preformulation composition containing a homogeneous mixture of a compound of the present invention, or a pharmaceutically acceptable salt thereof. When referring to these preformulation compositions as homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules This solid preformulation composition is then subdivided mto unit dosage forms of the type described above containing from 0.1 to about 500 mg of the active ingredient of the present invention. Typical unit dosage forms contain from 1 to 100 mg, for example 1, 2, 5, 10, 25, 50 or 100 mg, of the active ingredient The tablets or pills of the novel composition can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being m the form of an envelope over the former The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permits the inner component to pass intact into the duodenum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate The liquid forms in which the novel compositions of the present mvention may be incorporated for administration orally or by injection include aqueous solutions, suitably flavoured syrups, aqueous or oil suspensions, and flavoured emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil or peanut oil, as well as elixirs and similar pharmaceutical vehicles Suitable dispersing or suspending agents for aqueous suspensions include synthetic and natural gums such as tragacanth, acacia, alginate, dextran, sodium carboxymethylcellulose, methylcellulose, polyvinyl-pyrrolidone or gelatin. In the treatment of migraine, a suitable dosage level is about 0.01 to 250 mg/kg per day, preferably about 0.05 to 100 mg/kg per day, and especially about 0.05 to 5 mg kg per day. The compounds may be administered on a regimen of 1 to 4 times per day. The compounds according to the invention wherein -F-G- represents -CH2-N- may be prepared by a process which comprises attachment of the R 1 moiety to a compound of formula III: wherein Z, E, Q, T, U and V are as defined above; by conventional means including N-alkylation. Attachment of the R 1 moiety to the compounds of formula III may conveniently be effected by standard alkylation techniques. One example thereof comprises treatment with an alkenyl halide such as 4-bromobut-l- ene, 4-bromo-2-methylbut-2-ene or allyl bromide, an alkynyl halide such as propargyl bromide, or an aryl(Cι-G)alkyl or heteroaryl(Cι-G)alkyl halide such as benzyl iodide, typically under basic conditions, e.g. sodium hydride or potassium carbonate in N,N-dimethylformamide, or triethylamine in acetonitrile. Another example comprises treatment of the compound of formula III with an aryl(Cι-G)alkyl or heteroaryl(Cι-6)alkyl mesylate such as 2-(4-cyanophenyl)ethyl methanesulphonate or 2-(2- or 3-thienyl)ethyl methanesulphonate, typically in the presence of sodium carbonate or potassium carbonate and optionally sodium iodide, in a suitable solvent such as 1,2-dimethoxyethane or N,N-dimethylformamιde. Alternatively, the R 1 moiety may conveniently be attached by reductive alkylation, which may be accomplished in a smgle step, or as a two-step procedure. The single-step approach suitably comprises treating the required compound of formula III as defined above with the appropriate aldehyde, e.g. benzaldehyde, (3-fluorophenyl)acetaldehyde, furfuraldehyde or thiophene carboxaldehyde, in the presence of a reducing agent such as sodium cyanoborohydride. In a typical two-step procedure, for the preparation of a compound of formula I wherem R 1 corresponds to a group of formula -CH2R 11 , a carboxylic acid derivative of formula R n -CO 2 H is condensed with the required compound of formula III, suitably in the presence of l-(3-dιmethylamιnopropyl)-3-ethylcarbodmnιde hydrochloride and 1 -hydroxybenzotriazole hydrate, to afford a compound corresponding to formula I wherein R 1 represents -COR 11 , the carbonyl group thereof can then be reduced, for example by treatment with dusobutylalummium hydride, and the required compound of formula I thereby obtained The compounds of formula III above wherem T represents CH, U represents C-R 2 and V represents N-R 3 , corresponding to the dole derivatives of formula ID as defined above wherem T represents CH, -F-G represents -CH2-N- and R 1 is hydrogen, may be prepared by a process which comprises reacting a compound of formula IV (IV) wherein Z and E are as defined above, with a compound of formula V, or a carbonyl-protected form thereof (V) wherein R 2 and Q are as defined above, and RP represents an ammo- protecting group; followed, where required, by N-alkylation by standard methods to introduce the moiety R 3 ; with subsequent removal of the amino-protectmg group RP. The reaction between compounds IV and V, which is an example of the well-known Fischer indole synthesis, is suitably carried out by heating the reagents together under mildly acidic conditions, e.g. 4% sulphuric acid at reflux Suitable carbonyl-protected forms of the compounds of formula V include the dimethyl acetal or ketal derivatives The protecting group RP in the compounds of formula V is suitably a carbamoyl moiety such as tert-butoxycarbonyl (BOC), which can conveniently be removed as necessary by treatment under mildly acidic conditions. Indeed, the acidic conditions of the Fischer mdole synthesis reaction will generally suffice to remove the BOC group The Fischer reaction between compounds IV and V may be carried out in a single step, or may proceed via an initial non-cychsmg step at a lower temperature to give an intermediate of formula VI. (VI) wherein Z, E, Q, R 2 and RP are as defined above, followed by cyclisation using a suitable reagent, e g a polyphosphate ester The intermediates of formula V, or carbonyl-protected forms thereof, may be prepared by reacting a compound of formula VII, or a carbonyl- protected form thereof, with a compound of formula VIII: O / \ Λ / Q-L 1 H-N N-R p (VII) (VIII) wherein Q, R 2 and RP are as defined above, and L 1 represents a suitable leaving group. The leaving group L 1 is suitably a halogen atom, e.g. chlorine or bromine. Where L 1 represents a halogen atom, the reaction between compounds VII and VIII is conveniently effected by stirring the reactants under basic conditions in a suitable solvent, for example potassium carbonate m N,N-dimethylformamide, or triethylamine in tetrahydrofuran or acetonitrile. The compounds according to the invention wherem T represents CH, U represents C-R 2 and V represents Ν-R 3 - i.e. the indole derivatives of formula ID as defined above wherein T represents CH - may alternatively be prepared by a process which comprises reacting a compound of formula IV as defmed above with a compound of formula IX, or a carbonyl-protected form thereof: 0 / \ -Q- Ν G- R R- (IX) wherein Q, F, G, R 1 and R 2 are as defined above: under conditions analogous to those described above for the reaction between compounds IV and V; followed, where required, by N-alkylation by standard methods to introduce the moiety R 3 . As for the compounds of formula V, suitable carbonyl-protected forms of the compounds of formula IX include the dimethyl acetal or ketal derivatives. As with that between compounds IV and V, the Fischer reaction between compounds IV and IX may be carried out in a single step, or may proceed via an initial non-cyclising step at a lower temperature to give an intermediate of formula X: (X) wherein Z, E, Q, F, G, R 1 and R 2 are as defined above; followed by cyclisation using a suitable reagent, e.g. a polyphosphate ester. The intermediates of formula IX, or carbonyl-protected forms thereof, may be prepared by reacting a compound of formula VII as defined above, or a carbonyl-protected form thereof, with a compound of formula XI: / \ H- N G - R (Xi) wherein F, G and R 1 are as defined above; under conditions analogous to those described above for the reaction between compounds VII and VIII. In an alternative procedure, the compounds of formula III above may be prepared by a process which comprises reacting a compound of formula VIII as defined above with a compound of formula XII: (XII) wherein Z, E, Q, T, U and V are as defined above, and L 2 represents a suitable leaving group; followed by removal of the ammo-protectmg group Similarly, the compounds of formula I as defined above may be prepared by a process which comprises reacting a compound of formula XI as defined above with a compound of formula XII as defined above. The leaving group L 2 is suitably an alkylsulphonyloxy or arylsulphonyloxy group, e.g. methanesulphonyloxy (mesyloxy), trifluoromethanesulphonyloxy (triflyloxy) or p-toluenesulphonyloxy (tosyloxy). Where L 2 represents an alkylsulphonyloxy or arylsulphonyloxy group, the reaction between compound XII and compound VIII or XI is conveniently carried out in a suitable solvent such as 1.2- dimethoxyethane, isopropyl alcohol or N,N-dιmethylformamιde. typically in the presence of a base such as sodium carbonate or potassium carbonate, optionally with the addition of sodium iodide In a further procedure, the compounds of formula III above wherein T represents CH, U represents nitrogen and V represents Ν-R\ corresponding to the indazole derivatives of formula IB as defmed above wherein -F-G- represents -CH2-N- and R 1 is hydrogen, may be prepared by a process which comprises cychsing a compound of formula XIII (XIII) wherein Z, E, Q and RP are as defined above, and D 1 represents a readily displaceable group; followed, where required, by N-alkylation by standard methods to introduce the moiety R 3 ; with subsequent removal of the amino-protecting group RP. Similarly, the compounds of formula I wherein T represents CH, U represents nitrogen and V represents N-R 3 - i.e. the indazole derivatives of formula IB as defined above - may be prepared by a process which comprises cyclising a compound of formula XIV: (XIV) in which Z, E, Q, F, G, R 1 and D 1 are as defined above; followed, where required, by N-alkylation by standard methods to introduce the moiety R . The cyclisation of compounds XIII and XIV is conveniently achieved m a suitable organic solvent at an elevated temperature, for example in a mixture of -xylene and 2,6-lutidine at a temperature in the region of 140°C. The readily displaceable group D 1 in the compounds of formula XIII and XIV suitably represents a Cι- alkanoyloxy group, preferably acetoxy. Where D 1 represents acetoxy, the desired compound of formula XIII or XIV may be conveniently prepared by treating a carbonyl compound of formula XV: (XV) wherein Z, E, Q, F and G are as defined above, and R x corresponds to the group R 1 as defined above, or R x represents an amino-protecting group as defined for RP when -F-G- represents -CH2-N-; or a protected derivative thereof, preferably the N-formyl protected derivative; with hydroxylamine hydrochloride, advantageously in pyridine at the reflux temperature of the solvent; followed by acetylation with acetic anhydride, advantageously in the presence of a catalytic quantity of 4-dimethylamιnopyridιne, in dichloromethane at room temperature. The N-formyl protected derivatives of the intermediates of formula XV may conveniently be prepared by ozonolysis of the corresponding indole derivative of formula XVI: G - R (XVI) wherein Z, E, Q, F, G and R x are as defined above; followed by a reductive work-up, advantageously using dimethylsulphide. The mdole derivatives of formula XVI may be prepared by methods analogous to those described in the accompanying Examples, or by procedures well known from the art. In a still further procedure, the compounds of formula III above wherein T represents CH, U represents C-R 2 and V represents oxygen or sulphur, corresponding to the benzofuran or benzthiophene derivatives of formula IA wherein V is oxygen or sulphur respectively, -F-G- represents -CH2-N- and R 1 is hydrogen, may be prepared by a process which comprises cyclising a compound of formula XVII: (XVII) wherein Z, E, Q, R 2 and RP are as defined above, and V 1 represents oxygen or sulphur; followed by removal of the amino-protect g group RP. Similarly, the compounds of formula I wherein T represents CH, U represents C-R 2 and V represents oxygen or sulphur - i.e. the benzofuran or benzthiophene derivatives of formula IA above - may be prepared by a process which comprises cyclising a compound of formula XVIII: wherein Z, E, Q, F, G, R 1 , R 2 and V 1 are as defined above. The cyclisation of compounds XVII and XVIII is conveniently effected by using polyphosphoric acid or a polyphosphate ester, advantageously at an elevated temperature. The compounds of formula XVII and XVIII may be prepared by reacting a compound of formula XIX with a compound of formula XX: (XIX) (XX) wherein Z, E, Q, F, G, R 2 , V 1 and R x are as defined above, and Hal represents a halogen atom. The reaction is conveniently effected in the presence of a base such as sodium hydroxide. The hydroxy and mercapto derivatives of formula XIX may be prepared by a variety of methods which will be readily apparent to those skilled in the art. One such method is described in EP-A-0497512. In a yet further procedure, the compounds of formula III above may be prepared by a process which comprises reducing a compound of formula XXI: (XXI) wherein Z, E, T, U, V and RP are as defined above, and -Q 2 -CH 2 - corresponds to the moiety Q as defined above; with subsequent removal of the amino-protectmg group Rι>. Similarly, the compounds according to the invention may be prepared by a process which comprises reducing a compound of formula XXII: (XXII) wherein Z, E, T, U, V, F, G, R 1 and Q 2 are as defined above. The reduction of compounds XXI and XXII is conveniently effected by treating the appropriate compound with a reducmg agent such as lithium aluminium hydride in an appropriate solvent, e.g. diethyl ether or tetrahydrofuran, or mixtures thereof. The compounds of formulae XXI and XXII above may suitably be prepared by reacting a compound of formula XXIII with the appropriate compound of formula XXIV: (XXIII) (XXIV) wherein Z, E, T, U, V, F, G, R x and Q 2 are as defined above, and J represents a reactive carboxylate moiety. Suitable values for the reactive carboxylate moiety J include esters, for example Cι- alkyl esters; acid anhydrides, for example mixed anhydrides with Cι. 4 alkanoic acids; acid halides. for example acid chlorides; and acvhmidazoles. By way of example, the intermediates of formula XXIII above wherein J is an acid chloride moiety may be prepared by treating the corresponding carboxylic acid derivative with thionyl chloride in toluene. Similarly, the intermediates of formula XXIII wherein J is an acylimidazole moiety may be prepared by treating the corresponding carboxylic acid derivative with l,l'-carbonyldiimidazole. Alternatively, the reactive carboxylate moiety J may be obtained by treating the corresponding compound wherein J is carboxy with l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 1- hydroxybenzotriazole hydrate, optionally in the presence of triethylamine; the resulting activated carboxylate intermediate may then suitably be reacted in situ with the required compound of formula XXIV. In a still further procedure, the compounds of formula III above may be prepared by a process which comprises reacting a compound of formula XXV: (XXV) wherem Z, E, T, U, V and RP are as defined above, and Q 3 corresponds to the moiety Q as defined above in which the or each fluorine atom is replaced by a leaving group; with an available source of fluoride ion; with subsequent removal of the amino-protecting group RP. Similarly, the compounds according to the invention may be prepared by a process which comprises reacting a compound of formula XXVI: (XXVI) wherein Z, E, T, U, V, F, G, R 1 and Q 3 are as defined above; with an available source of fluoride ion. The source of fluoride ion for use in the above reaction with compounds XXV and XXVI is suitably tetra-n-butylammonium fluoride, in which case the reaction is conveniently carried out in a solvent such as N,N-dimethylformamide at an elevated temperature. The leaving group present in the moiety Q 3 in compounds XXV or XXVI is suitably an alkylsulphonyloxy or arylsulphonyloxy group such as mesyloxy or tosyloxy, preferably mesyloxy. Where this group is mesyloxy, the appropriate compound of formula XXV or XXVI can conveniently be obtained by treatment of the corresponding hydroxy compound with mesyl chloride in the presence of triethylamine, in a solvent such as tetrahydrofuran. The required hydroxy precursor can in turn be prepared by methods analogous to those described above for the preparation of the compounds of formula III and I respectively. The hydrazine derivatives of formula IV above may be prepared by methods analogous to those described in EP-A-0438230, EP-A-0497512, EP-A-0548813 and WO-A-91/18897. Where they are not commercially available, the starting materials of formula VII, VIII, XI, XII, XX and XXIV may be prepared by methods analogous to those described in the accompanying Examples, or by standard procedures well known from the art. It will be understood that any compound of formula I initially obtained from any of the above processes may, where appropriate, subsequently be elaborated into a further compound of formula I by techmques known from the art. For example, a compound of formula I wherein -F-G- represents -CH=C- initially obtained may be readily converted into the corresponding compound wherein -F-G- represents -CH2-CH- by conventional catalytic hydrogenation procedures. In addition, a compound of formula I wherem R 1 is benzyl initially obtained may be converted by catalytic hydrogenation to the corresponding compound of formula III, which in turn may be converted into a further compound of formula I using standard N-alkylation techniques as described above. Furthermore, a compound of formula I initially obtained wherein the R 1 moiety is substituted by nitro or cyano may be converted by catalytic hydrogenation to the corresponding ammo- or am omethyl- substituted compound respectively. Additionally, a compound of formula I wherein the R 1 moiety is substituted by hydroxy, possibly obtained by lithium aluminium hydride reduction of a precursor alkoxycarbonyl derivative, may be mesylated under standard conditions, and the mesyl group subsequently displaced by an amino moiety by treatment with the desired amine in a sealed tube at an elevated temperature. The amme derivative resulting from any of these procedures may then, for example, be N-acylated using the appropriate acyl halide, e g acetyl chloride; or aminocarbonylated, using potassium isocyanate, to the corresponding urea derivative; or converted to a l,2,4-tπazol-4-yl derivative using N,N- dimethylformamide azme, or reductively alkylated by treatment with the appropriate aldehyde or ketone in the presence of sodium cyanoborohydride If desired, the amine derivative may also be carbamoylated by treatment with the requisite alkyl chloroformate A compound of formula I initially obtained wherein the R 1 moiety is substituted by cyano may be converted, by treatment with sodium azide, to the corresponding tetrazole derivative, which in turn may be alkylated on the tetrazole ring by treatment with an alkyl hahde under standard conditions By way of additional illustration, a compound of formula I initially obtained wherein the R 1 moiety is substituted by an alkoxycarbonyl moiety may be saponified, by treatment with an alkali metal hydroxide, to the corresponding carboxy-substituted compound, which in turn may be converted to an amide derivative by treatment with the appropriate amine, advantageously in the presence of l-(3- dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 1- hydroxybenzotriazole. Moreover, a compound of formula I wherein R 3 is hydrogen initially obtained may be converted into a compound of formula I wherein R 3 represents Cι-6 alkyl by standard alkylation techniques, for example by treatment with an alkyl iodide, e.g. methyl iodide, typically under basic conditions, e.g. sodium hydride in N,N-dimethylformamide. Where the above-described processes for the preparation of the compounds according to the invention give rise to mixtures of stereoisomers, these isomers may be separated by conventional techniques such as preparative chromatography. The novel compounds may be prepared in racemic form, or individual enantiomers may be prepared either by enantiospecific synthesis or by resolution. The novel compounds may, for example, be resolved into their component enantiomers by standard techniques such as preparative HPLC, or the formation of diastereomeric pairs by salt formation with an optically active acid, such as (-)-di- -toluoyl-d-tartaric acid and/or (+)-di-p-toluoyl-l-tartaric acid, followed by fractional crystallization and regeneration of the free base. The novel compounds may also be resolved by formation of diastereomeric esters or amides, followed by chromatographic separation and removal of the chiral auxiliary. During any of the above synthetic sequences it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene & P. G.M. Wuts, Protective Groups in Organic Synthesis, John Wiley & Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from the art. The following Examples illustrate the preparation of compounds according to the invention. The compounds in accordance with the present invention potently and selectively bind to the 5-HTiD α receptor subtype, inhibit forskolin- stimulated adenylyl cyclase activity, and stimulate [ 35 S]-GTPγS binding to membranes from clonal cell lines expressing human cloned receptors. 5-HTiD α /5-HTiD β Radioligand Binding Chinese hamster ovary (CHO) clonal cell lines expressing the human 5-HTiD α and 5-HTiD p receptors were harvested in PBS and homogenised in ice cold 50 mM Tris-HCl (pH 7.7 at room temperature) with a Kinematica polytron and centrifuged at 48,000g at 4°C for 11 mm. The pellet was then resuspended in 50 mM Tns-HCl followed by a 10 min incubation at 37°C. Finally the tissue was recentnfuged at 48,000g, 4°C for 11 mm and the pellet resuspended, m assay buffer (composition in mM: Tris-HCl 50, pargyline 0.01, CaCl 2 4; ascorbate 0.1%; pH 7.7 at room temperature) to give the required volume immediately prior to use (0.2 mg protem/ml). Incubations were carried out for 30 mm at 37°C the presence of 0.02-150 nM [ 3 H]-5-HT for saturation studies or 2-5 nM [ 3 H]-5- HT for displacement studies. The final assay volume was 1 ml. 5-HT (10 μM) was used to defme non-specific binding. The reaction was initiated by the addition of membrane and was terminated by rapid filtration through Whatman GF/B filters (presoaked in 0 3% PEl/ 0.5% Triton X) followed by 2 x 4 ml washings with 50 mM Tris-HCl. The radioactive filters were then counted on a LKB beta or a Wallac beta plate counter Binding parameters were determined by non-linear, least squares regression analysis using an iterative curve fitting routine, from which I do (the molar concentration of compound necessary to inhibit binding by 50%) values could be calculated for each test compound. The IC50 values for binding to the 5-HTiD α receptor subtype obtamed for the compounds of the accompanying Examples were below 50 nM in each case. Furthermore, the compounds of the accompanying Examples were all found to possess a selective affinity for the 5-HTiD α receptor subtype of at least 10-fold relative to the 5-HTID P subtype. 5-HTiD α /5-HTiD P Adenylyl Cyclase Assay Studies were performed essentially as described J. Pharmacol Exp. Ther., 1986, 238, 248. CHO clonal cell lines expressing the human cloned 5-HTiD α and 5-HT p receptors were harvested in PBS and homogenised, using a motor driven teflon/glass homogeniser, in ice cold Tris HC1-EGTA buffer (composition m mM. Tris HCl 10, EGTA 1, pH 8.0 at room temperature) and incubated on ice for 30-60 min. The tissue was then centrifuged at 20,000g for 20 mm at 4°C, the supernatant discarded and the pellet resuspended in Tris HC1-EDTA buffer (composition in mM Tris HCl 50, EDTA 5, pH 7.6 at room temperature) just prior to assay The adenylyl cyclase activity was determined by measuring the conversion of α-[ 3 P]-ATP to [ 33 P]-cychc AMP. A 10 μl aliquot of the membrane suspension was incubated, for 10-15 mm, in a final volume of 50 μl, at 30°C, with or without forskolm (10 μM), in the presence or absence of test compound. The incubation buffer consisted of 50 mM Tris HCl (pH 7 6 at room temperature), 100 mM NaCl, 30 μM GTP, 50 μM cyclic AMP, 1 mM dithiothreitol, 1 mM ATP, 5 mM MgCl 2 , 1 mM EGTA, 1 mM 3-ιsobutyl-l- methylxanthme, 3 5 mM creatmine phosphate, 0.2 mg/ml creatine phosphokinase, 0.5-1 μCi α-[ 33 P]-ATP and 1 nCi [ H]-cychc AMP The incubation was initiated by the addition of membrane, following a 5 mm premcubation at 30°C, and was terminated by the addition of 100 μl SDS (composition in mM sodium lauryl sulphate 2%, ATP 45 cyclic AMP 1 3 pH 7 5 at room temperature) The ATP and cyclic AMP were separated on a double column chromatography system (Anal. Biochem., 1974, 58, 541). Functional parameters were determined using a least squares curve fitting programme ALLFIT (Am. J. Physiol, 1978, 235, E97) from which E m a (maximal effect) and EC50 (the molar concentration of compound necessary to inhibit the maximal effect by 50%) values were obtained for each test compound. Of those compounds which were tested in this assay, the EC50 values for the 5-HTID„ receptor obtained for the compounds of the accompanying Examples were below 500 nM in each case. Moreover, the compounds of the accompanying Examples which were tested were all found to possess at least a 10-fold selectivity for the 5-HTιo α receptor subtype relative to the 5-HT p subtype. 5-HTiD α /5-HTiD β GTPγS Binding Studies were performed essentially as described m Br. J. Pharmacol, 1993, 109, 1120. CHO clonal cell lines expressing the human cloned 5-HTι.D α and 5-HTiDp receptors were harvested in PBS and homogenised using a Kinematica polytron in ice cold 20 mM HEPES containing 10 mM EDTA, pH 7.4 at room temperature. The membranes were then centrifuged at 40,000g, 4°C for 15 min. The pellet was then resuspended in ice cold 20 mM HEPES contaming 0.1 mM EDTA, pH 7.4 at room temperature and recentrifuged at 40,000g, 4°C for 15-25 minutes. The membranes were then resuspended in assay buffer (composition in mM: HEPES 20, NaCl 100, MgCl 2 10, pargyline 0.01; ascorbate 0.1%; pH 7.4 at room temperature) at a concentration of 40 μg protein/ml for the 5-HTiD α receptor transfected cells and 40-50 μg protein/ml for the 5-HTiD p receptor transfected cells. The membrane suspension was then incubated, in a volume of 1 ml, with GDP (100 μM for 5-HTiD α receptor transfected cells. 30 μM for the 5-HT^ β receptor transfected cells) and test compound at 30°C for 20 mm and then transferred to ice for a further 15 min. [ 35 S]-GTPγS was then added at a final concentration of 100 pM and the samples incubated for 30 min at 30°C The reaction was initiated by the addition of membrane and was terminated by rapid filtration through Whatman GF/B filters and washed with 5 ml water. The radioactive filters were then counted on a LKB beta counter. Functional parameters were determined by a non-linear, least squares regression analysis using an iterative curve fitting routine, from which E m ax (maximal effect) and EC50 (the molar concentration of compound necessary to inhibit the maximal effect by 50%) values were obtained for each test compound. Of those compounds which were tested in this assay, the EC50 values for the 5-HTiD α receptor obtained for the compounds of the accompanying Examples were below 500 nM in each case. Moreover, the compounds of the accompanying Examples which were tested were all found to possess at least a 10-fold selectivity for the 5-HT I D Q receptor subtype relative to the 5-HTiDp subtype INTERMEDIATE 1 4-(l,2,4-Trιazol-4-yl)phenylhvdrazιne Prepared as described in WO 94/03446. INTERMEDIATE 2 3-Fluorophenylacetaldehyde a) l-(3-Fluorophenyl)-2-methoxyethene Phenylhthium (98 3ml of a 1.8M solution in cyclohexane/diethyl ether, 177mmol) was added to a stirred suspension of (methoxymethyl)trιphenylphosphonιum chloride (60 82g, 177mmol) (dried overnight at 50°C in vacuo prior to use), in diethyl ether (500ml) at 0°C under nitrogen The solid was seen to dissolve and a bright orange brown coloration formed The mixture was stirred at 0°C for 0.25h and then at room temperature for 0.5h. The mixture was cooled to -20°C and 3-fluorobenzaldehyde (20.0g, 161mmol) then added. The reaction mixture was allowed to warm to room temperature and stirred overnight (16h). Saturated ammonium chloride solution (250ml) was added and the aqueous phase separated and extracted further with diethyl ether (x2). The combined etheral layers were dried (MgSO ), evaporated in vacuo and the residue distilled («60°C at 1.3mbar) to remove the majority of the phosphorous by-products. The distillate was then purified by column chromatography on silica gel, eluting with ethyl acetate/hexane (2:98) to give the title-enol ether as an E/Z mixture (10.7g, 44%), δ H (250MHz,CDCl 3 ) 3.69 and 3.80 (total 3H, 2 of s, OCH ), 5.21 and 5.77 (total IH, 2 of d, J=7.0Hz and 13.0Hz respectively, C=CHz and C=CH E ), 6.18 (d, J=7.0Hz, C=CHz), 6.78-7.41 (m, Ar-H and C=CH E ). b) 3-Fluorophenylacetaldehvde Concentrated hydrochloric acid (45ml) was added to a stirred solution of the preceding enol ether (7.22g, 47.5mmol) in THF (225ml), at 0°C. The mixture was stirred under nitrogen for 0.3h at 0°C, and then at room temperature for 3h. Water was added and the volatiles were evaporated in vacuo. The residue was taken up in diethyl ether and the organic layer separated, washed with water (x2), saturated sodium bicarbonate solution (xl) and water (xl), dried (MgSO 4 ) and evaporated in vacuo to afford the title-aldehyde (5.81g, 89%), δ (250MHz, CDC1 ) 3.71 (2H, d, J=2.2Hz, CH 2 ), 6.93-7.39 (4H, , Ar-H), 9.76 (IH, t. J=2.1Hz, CHO). INTERMEDIATE 3 (R,S)-2-Fluoro-3-[5-(l,2.4-trιazol-4-yl)-lH-mdol-3-yllproDan- l-ol a) (R,S)-2-Fluoro-5-hexen-l-ol To a cooled (-10°C) and stirred mixture of hydrogen fluoride- pyridine (70% HF; 10ml) and anhydrous dichloromethane (60ml), in a polypropylene tube, was added dropwise via syringe neat l,2-epoxy-5- hexene (13.8ml) over 20 minutes, under nitrogen. After a further 20 minutes, the yellow solution was carefully poured into ice-water- concentrated ammonia (57ml of ammonia; total volume 400ml) and the aqueous mixture was saturated with sohd sodium chloride. Products were extracted with diethyl ether (2x500ml) and the combined ethereal solutions were washed with brine (45ml), brme - 10% aqueous sodium bicarbonate (5:1, 60ml) then dried (MgSO ) and concentrated (bath temperature 30°C). Flash chromatography to the residual liquid (sihca gel, hexane - diethyl ether, 65:35) followed by purification on alumina (activity III, dichloromethane), gave 4.4g of the title compound as a colourless liquid; δ H (360MHz, CDC1 3 ) 1.56-1.90 (2H, m), 2.10-2.30 (2H, m), 3.62-3.80 (2H, m), 4.48-4.70 (IH, dm, J=50Hz), 5.00-5.12 (2H, m), 5.75- 5.88 (IH, m). b) (R,S)-6-tert-Butyldimethylsiiyloxy-5-fluoro-l-hexene To a stirred solution of 2-fluoro-5-hexen-l-ol (4.4g, 37.2mmol) in anhydrous dimethylformamide (125ml) were added imidazole (7.60g, 111.7mmol) and tert-butyldimethylsilyl chloride (8.42g, 55.9mmol), under nitrogen. After being stirred at room temperature for 7.5 hours, the mixture was diluted with diethyl ether (400ml) and it was washed with water (150ml), IM hydrochloric acid (100ml), 5% aqueous sodium bicarbonate (100ml), brine (100ml), then dried (MgSO 4 ) and concentrated. Flash chromatography of the residue (silica gel, petrol ether (40-60) to petrol ether (40-60) - 5% diethyl ether) gave the title compound as a colourless liquid. Some impure fractions were repurified on alumina (activity III; petroleum ether (40-60)); total yield of product 6.72g (77.7%). δ H (360MHz, CDCls) 0.07 (6H, s), 0.90 (9H, s), 1.58-1.84 (2H, m), 2.10-2.30 (2H, m), 3.71 (2H, dd, J=22.2 and 4.7Hz), 4.40-4.60 (IH, dm, J=49Hz), 4.96-5.10 (2H, m), 5.75-5.88 (IH, m). c) (R.S)-5-tβrt-Butyldimethylsilyloxy-4-fluoropentanal Ozone was bubbled through a cooled (-75°C) and stirred solution of the preceding olefin (6.7g, 28.8mmol) in anhydrous dichloromethane (150ml) until a blue colour persisted (45 minutes). Oxygen was then bubbled for 10 minutes through the solution before it was kept under a nitrogen atmosphere. Anhydrous dimethyl sulfide (10ml, 136.2mmol) was added at -78°C and the mixture was allowed to warm to room temperature. After 2 hours, solvents were removed under vacuum (bath temperature 32°C) and the residue was purified by flash chromatography (silica gel, hexane - diethyl ether, 80:20) to give 5.45g of the intermediate ozonide and 1 g of the required title product, as pale pmk liquids The ozonide (5.4g, 19.28mmol) in anhydrous dichloromethane (125ml) was cooled to -78°C, under nitrogen, and anhydrous triethylamine (5 4ml, 38.6mmol) was added over 2 minutes. The resulting solution was allowed to warm to room temperature and stirred for 2.5 hours. The mixture was half concentrated under vacuum and directly chromatographed on silica gel (dichloromethane) to give 3.60g of the title compound as a colourless liquid; δ H (360MHz, CDCh) 0.07 (6H, s), 0.90 (9H, s), 1.90-2.05 (2H, m), 2.54-2.72 (2H, m), 3.73 (2H, dd, J=21.3 and 4.5Hz), 4.40-4.62 (IH, dm, J=49.5Hz), 9 81 (IH, dd, J=2.1 and 0.9Hz) d) (R.S)-2-Fluoro-3-[5-(1.2.4-trιazol-4-yl)-lH-mdol-3-yllpropan-l-oI To a stirred solution of the preceding aldehyde (4 60g, 19 6mmol) in dioxane (170ml) was added 4-(l,2,4-trιazol-4-yl)phenylhvdrazme (3.80g, 21.1mmol) followed by water (20ml). After 15 minutes. 2M hydrochloric acid (11ml) was added and the mixture was refluxed for 68 hours under nitrogen. Solvents were removed under vacuum and the residue was purified by flash chromatography (sihca gel, dichloromethane - methanol, 90:10), then alumina (activity III, dichloromethane - methanol - ammonia, 95:5:0.5; then dichloromethane - methanol - ammonia, 90:10:1), to give 700mg of the title compound as a yellow sohd; δH (360MHz, DMSO-dβ) 2.98-3.10 (2H, m), 3.44-3.66 (2H, m), 4.66-4.88 (IH, dm, J=49Hz), 4.93 (IH, t, J=5.6Hz), 7.32 (IH, dd, J=8.5 and 1.9Hz), 7.35 (IH, d, J=2.1Hz), 7.50 (IH, d, J=8.5Hz), 7.82 (IH, d, J=1.9Hz), 9.00 (2H, s), 11.19 (IH, s); m/e (ES) 261 (M + +l). EXAMPLE 1 4-Benzyl-l-{(R.S -2-(fluoromethyl)-3-f5-(1.2.4-trιazol-4-yl)-lH-ιndol-3- yllpropyllpiperazine. 2.5 Hvdrogen Oxalate 0.4 Hydrate a) 4-BenzyI- l-f(ethoxycarbonyl)acetyl1 piperazine To a cooled (-20°C) and stirred solution of N-benzylpiperazine (lOg, 56.7mmol) and triethylamine (8.7ml, 62.4mmol) m dichloromethane (200ml) was added ethyl malonyl chloride (8.5ml, 66.4mmol) over 10 minutes, under nitrogen. The mixture was allowed to warm to room temperature and it was stirred for 1 hour before water (100ml) and diethyl ether (500ml) were added. The organic phase was decanted off, washed with water (1x70ml), 10% aqueous sodium bicarbonate (1x75ml), brine (1x75ml), then dried (MgSO ) and concentrated. Flash chromatography of the residue (sihca gel, ethyl acetate - ethanol, 95:5) afforded 9.53g (58%) of the title compound as a colourless oil; δH (360MHz, CDC1 3 ), 1.28 (3H, t, J=7.2Hz), 2.48 (4H, br s), 3.45 (2H, s), 3.47 (2H, br s), 3.56 (2H, br s), 3 68 (2H, br s), 4.19 (2H, q, J=7.2Hz), 7.24-7.36 (5H, m); m/e (ES) 291 (M + +l) b) (R,S)-4-Benzyl-l-[4-(1.3-dιoxolan-2-ylV2-(ethoxycarbonyl)- butyryl pιperazιne To a stirred suspension of sodium hydride (60% dispersion m oil, 1.4g) in anhydrous dimethylformamide (50ml) was added dropwise, under nitrogen, a solution of 4-benzyl-l-[(ethoxycarbonyl)acetyl]piperazine (8.5g, 29.3mmol) in anhydrous dimethylformamide (50ml) over 25 minutes at room temperature. After a further 30 minutes at room temperature, 2-(2- bromoethyl)-l,3-dioxolane (3.6ml, 30.7mmol) was added dropwise over 3 minutes and the resulting yellow solution was stirred for 24 hours. Water (400ml) was added and products were extracted with diethyl ether (3x300ml). The combined ethereal phases were washed with brine (1x150ml), dried (MgSO 4 ) and concentrated. Flash chromatography of the residue (silica gel, diethyl ether - ethanol, 95:5) afforded 8g of the title compound (70%) as a colourless oil; δH (360MHz, CDC1 3 ) 1.25 (3H, t, J=7.1Hz), 1.56-1.78 (2H, m), 2.06 (2H, q, J=7.4Hz), 2.44 (4H, br s), 3.46- 3.96 (11H, m), 4.16 (2H, q, J=7.1Hz), 4.86 (IH, t, J=4.6Hz), 7.24-7.36 (5H, m); m/e (ES) 391 (M + +l). c) (R.S)-4-Benzyl-l-f4-(1.3-dioxolan-2-yl)-2-(hvdroxymethyl)- butyl]piperazine To a cooled (-30°C) and stirred solution of lithium aluminium hydride (IM in THF; 16.4ml) in anhydrous tetrahydrofuran (30ml) was added dropwise, via cannula, a solution of the product from the preceding step (3.2g, 8.19mmol) in anhydrous tetrahydrofuran (30ml) over 14 minutes, under a nitrogen atmosphere. The resulting clear colourless solution was allowed to warm to room temperature and it was stirred for 4 hours before excess lithium aluminium hydride was destroyed by careful addition of tetrahydrofuran-water (80:20; 25ml) (CAUTION! hydrogen evolution). The precipitated aluminium salts were filtered off, washed with tetrahydrofuran - water (80:20; 2x70ml) and the filtrate was concentrated under vacuum. Flash chromatography of the residue (silica gel, diethyl ether-methanol, 92:8; and silica gel, dichloromethane- methanol, 93:7) afforded 1.96g (71.5%) of the title compound as a colourless oil, δ H (360MHz, CDC1 3 ) 1.14-1.24 (2H, m), 1.62-1 72 (2H, m), 1.92-2.06 (IH, m), 2.20-2.90 (10H, m), 3 42-3.56 (3H, m), 3.72-4 00 (5H, m), 4 81 (IH, t, J=4 6Hz), 7.20-7 36 (5H, m), m/e (ES) 335 (M + +l) d) 4-Benzyl-l-((R.S)-2-(hvdroxymethyl)-3-r5-(1.2.4-trιazol-4-yl)-lH- ιndol-3-yl]propyl}piperazιne. 2.25 Hvdrogen Oxalate A solution of the preceding acetal (1.9g, 5 68mmol) and 4-(l,2,4- tπazol-4-yl)phenylhydrazme (l.lg, 6 25mmol) in 4% aqueous sulfuric acid (80ml) was stirred at room temperature for 20 minutes, then refluxed for 45 hours. After cool g, the mixture was basified with 30% aqueous sodium hydroxide, diluted with ethyl acetate (150ml) and it was vigorously stirred for 1 5 hours The organic phase was separated and the aqueous layer was extracted with ethyl acetate (3x300ml) The combined organic solutions were washed with br e (50ml), dried (MgSO ) and concentrated Flash chromatography of the residue (silica gel, dichloromethane - methanol - ammonia, 92-8 0.8) gave 1.2g (49%) of the title compound free base as a white foam The oxalate salt was prepared from absolute ethanol, mp 188-191°C (Found C, 55 94, H, 5 69, N, 13 28 dsHioNcO x 2 25 C2H2O4 requires C, 55 96, H, 5.49, N, 13 27%) δ H (360MHz, DMSO- db) 2 14-2 24 (IH, m), 2 64-3 10 (12H, m), 3 39 (IH, dd, J=10 6 and 6 OHz) 3 45 (IH, dd, J=10 6 and 4 3Hz), 3 77 (2H, m), 7 26-7 42 (7H, m), 7 49 (IH, d, J=8 6Hz), 7 78 (lH, d, 1 9Hz), 8 99 (2H, s), 11 17 (IH, s), m/e (ES) 431 (M + +l) e) 4-Benzyl-l-{(R.S)-2-(fluoromethyl)-3-[5-(1.2,4-trιazol-4-vD-lH-ιndo1- 3-vnpropyl)pιperazιne 2 5 Hvdrogen Oxalate 0 4 Hydrate To a cooled (-20°C) and stirred solution of 4-benzyl-l-{(R,S)-2- (hydroxymethyl)-3-[5-(l,2,4-tnazol-4-yl)-lH-mdol-3-yl]propyl}pιperazme (135mg, 0 31mmol) and anhydrous triethylamine (89μl, 0 64mmol) in anhydrous tetrahydrofuran (3ml) was added dropwise under nitrogen, methanesulphonvl chloride (50μl 0 64mmol) The resulting mixture was stirred at -20°C to -10°C for 1 hour 15 minutes before it was diluted with ethyl acetate (70ml) and washed with brine (2x20ml), then dried (MgSO ) and concentrated (bath temperature 36°C). The remaining residue was dissolved in anhydrous dimethylformamide (δml) and tetrabutylammonium fluoride (615mg, 2.35mmol) was added. The resultmg solution was heated at 60-70°C for 5 hours, under nitrogen. After cooling, water (50ml) and saturated aqueous potassium carbonate (5ml) were added and products were extracted with ethyl acetate (2x70ml). The combined organic solutions were washed with brine (1x35ml), dried (MgSO ) and concentrated. Flash chromatography of the residue (silica gel, dichloromethane - methanol - ammonia, 95:5:0.5) afforded 28mg of the title compound free base. The oxalate salt was prepared from ethanol- diethyl ether, mp 191-193°C. (Found: C, 54.14; H, 5.36; N, 12.49. C25H29FN6 x 2.5C2H2O4 x 0.4H 2 O requires: C, 54.20; H, 5.28; N, 12.64%) δ H (360MHz, DMSO-de) 2.24-2.43 (IH, m), 2.56-3.00 (12H, m), 4.01 (2H, s), 4.28-4.54 (2H, m), 7.30-7.48 (7H, m), 7.51 (IH, d, J=8.5Hz), 7.76 (IH, d, J=1.9Hz), 9.00 (2H, s), 11.21 (IH, s); m/e (ES) 433 (M + +l) EXAMPLE 2 4-f2-(3-Fluorophenyl)ethyll-l-{(R.S)-2-fluoro-3-[5-(1.2.4-trιazol-4-yl)-lH- ιndol-3-yllpropyllpiperazιne. 2.4 Hvdrogen Oxalate a) Ethyl (R.S)-4.5-epoxypentanoate To a cooled (-5°) and stirred solution of ethyl pent-4-enoate (lOg, 78mmol) in dichloromethane (200ml) was added dropwise a solution of m- chloroperoxybenzoic acid (50-55%; 29.5g) dichloromethane (250ml), over 30 minutes. The mixture was allowed to warm to room temperature overnight before it was diluted with diethyl ether (700ml) and washed with 2N aqueous sodium hydroxide (150ml), 10% sodium thiosulphate - 10% sodium iodide - 2N sodium hydroxide mixture (1:1 1, 2x80ml) and brme (2x100ml), then dried (MgSO 4 ) and concentrated Flash chromatography (sihca gel, dichloromethane to dichloromethane - diethyl ether, 1:1) of the residue afforded lOg (89%) of the title compound as a colourless liquid; δ H (360MHz, CDC1 3 ) 1.27 (3H, t, J=7.lHz), 1.79 (IH, qn, J=7.0Hz), 1.97 (IH, m), 2.46 (2H, t, J=7.2Hz), 2.51 (IH, m), 2.77 (IH, m), 2.99 (IH, m), 4.15 (2H, q, J=7.lHz). (R.S)-γ-[(4-tert-Butyloxycarbonylpιperazm-l-yl)methyl1-butyrolactone A solution of the preceding epoxide (6.33g, 43.9mmol) and 4-tert- butyloxycarbonylpiperazine (16.35g, 87 7mmol) in toluene (100ml) was heated at 90°C for 67 hours, under a nitrogen atmosphere The solvent was removed under vacuum and the residue was dissolved in diethyl ether (500ml) and washed with water (50ml), brine (75ml), then dried (Na2SO ) and concentrated Flash chromatography of the residue [silica gel, diethyl ether - ethanol (90.10) to diethyl ether - methanol (90 10) to dichloromethane - methanol - ammonia (95:5:0 5)] gave 5.35g (42.8%) of the title compound as a thick oil which solidified on standing; δH (250MHz, CDCI3) 1.46 (9H, s), 1.87-2.04 (IH, m), 2.25-2.38 (IH, m), 2.42-2.66 (8H, m), 3.44 (4H, t, J=5 OHz), 4.62-4 74 (IH, m), m/e (ES) 285 (M + +l) c) l-tert-Butyloxycarbonyl-4-((R.S)-2-hvdroxy-3-r5-(l,2.4-trιazol-4-v - lH-mdol-3-yl]propyl}pιperazme To a cooled (-80°C) and stirred solution of the preceding lactone (5 35g, 18.8mmol) in anhydrous toluene (200ml) was added dropwise, via cannula, a solution of dnsobutylaluminium hydride (1 5M in toluene, 20ml) over 35 minutes, under nitrogen. After being stirred at -82°C for 2 hours 20 minutes, the reaction was quenched by dropwise addition of methanol (15ml) followed by aqueous potassium sodium tartrate (20%, 200ml) and ethyl acetate (100ml) The mixture was vigorously stirred while warming to room temperature and the organic phase was decanted off The aqueous laver was extracted with ethvl acetate (3x250ml) and the combined organic solutions were washed with brme (50ml), then dried (MgSO ) and concentrated Flash chromatography of the residue (sihca gel, diethyl ether - methanol, 90:10 to 85:15) gave 4.60g (85.5%) of the intermediate lactol as a thick pale yellow oil, m/e (ES) 287 (M + +l) A solution of the above lactol (4.60g, 16.1mmol) and 4-(l,2,4-trιazol- 4-yl)phenylhydrazme (3 09g, 17.7mmol) in 4% sulfuric acid (200ml) was stirred at room temperature for 1 hour and then refluxed for 16 5 hours, under nitrogen After cooling, the mixture was basified with sohd potassium hydroxide and products were extracted with n-butanol (2x250ml) The combined organic solutions were concentrated under vacuum and the residue was dissolved in a mixture of tetrahydrofuran (200ml) and isopropanol (50ml). Di-tert-butyl dicarbonate (3 5g, 16 lmmol) was added and the mixture was stirred for 1 hour before it was basified (to pH 12) with 2N sodium hydroxide. Di-tert-butyl dicarbonate (lg) was added and stirring was continued for 30 minutes before solvents were removed under vacuum The remaining residue was diluted with water (200ml) and extracted with hexane (2x150ml) The aqueous layer was extracted with ethyl acetate (4x250ml) and the combined extracts were washed with brme (100ml), dried (MgSO ) and concentrated Flash chromatography of the residue (silica gel, dichloromethane - methanol - ammonia, 95 5 0 5, and silica gel, dichloromethane - methanol - ammonia, 92 8 0 8) afforded 1 57g of the title compound as a yellow foam, δH (360MHz, CDCLs) 1 45 (9H, s), 2.32-2 48 (4H, m), 2 56-2 64 (2H, m), 2 89 (2H, d, J=5 9Hz), 3 36-3 50 (4H, m), 4 00-4 08 (IH, m), 7 14 (IH dd, J=5 8 and 2 1Hz), 7 27 (IH, d J=2 3Hz), 7 48 (IH, d, J=8 5Hz), 7 66 (IH, d, J=2 1Hz), 8 47 (2H, s), 8 68 (IH, br s), m/e (ES) 427 (M + +l) d) 4-r2-(3-Fluorophenyl)ethyll-l-((R.S)-2-fluoro-3-r5-(1.2.4-trιazol-4-yl)- lH-indol 3-vnpropyl|pιperazιne 2 4 Hvdrogen Oxalate To a cooled (-10°C) and stirred solution of the preceding secondary alcohol (500mg, 1 17mmol) and anhydrous triethylamine (326μl 2.34mmol) in anhydrous tetrahydrofuran (10ml) was added methanesulphonyl chloride (185μl, 2.34mmol) over 2 minutes, under nitrogen. The resulting cloudy solution was stirred at -10°C for 0.5 hours and at room temperature for 2 hours before it was diluted with ethyl acetate (125ml), washed with brine-water (1:1, 30ml), brine (30ml), dried (MgSO 4 ), and concentrated. The residual yellow foam was dissolved in anhydrous dimethylformamide (15ml), solid tetrabutylammonium fluoride (2.70g) was added, and the resulting solution was heated at 70°C for 7 hours, under nitrogen. After cooling, the mixture was diluted with water (100ml), saturated aqueous potassium carbonate (3ml) was added, and products were extracted with ethyl acetate (2x100ml). The combined organic phases were washed with brine (60ml), dried (MgSO 4 ) and concentrated. Flash chromatography of the residue (sihca gel, dichloromethane - methanol - ammonia, 95:5:0.5) gave 57mg (11.4%) of 1- tert-butyloxycarbonyl-4-{(R,S)-2-fluoro-3-[5-(l,2,4-triazol-4-yl)-lH-indol-3- yl]propyl}piperazine; m/e (ES) 429 (M + +l). A solution of the above fluoro compound (55mg, 0.128mmol) in methanol (δml) and concentrated hydrochloric acid (3ml) was allowed to stand at room temperature for 1.5 hours. Solvents were removed under vacuum and the residue was azeotroped with methanol (2x10ml) to give a pale brown foam (60mg). This was dissolved in methanol (4ml) and sodium methoxide (30% w/v solution in methanol; 49μl) was added, followed by glacial acetic acid (37μl, 0.64mmol). A solution of 3- fluorophenylacetaldehyde (22mg, 0.16mmol) in methanol (1ml) was added followed, after 10 minutes, by sodium cyanoborohydride (15mg). The resulting solution was stirred at room temperature for 6 hours before more 3-fluorophenylacetaldehyde (17mg) and sodium cyanoborohydride (llmg) were added. Stirring was continued for a further 16 hours before sodium borohydride (30mg) was added and stirring was continued for 15 minutes. 4N Sodium hydroxide (4ml) was then added and the methanol was removed under vacuum. The remaining residue was diluted with water (10ml) and brine (δml), and products were extracted with ethyl acetate (2x40ml). The combined organic phases were washed with brme (25ml), dried (MgSO ) and concentrated. Preparative TLC purification (sihca gel, dichloromethane - methanol - ammonia, 95:5:0.5) of the residue afforded 5 34mg of the title compound free base. The oxalate salt was prepared from ethanol-diethyl ether, mp 193-19δ°C. (Found: C, 53.65; H, 5.11; N, 12.5δ. C25H28F2N6 x 2.4C2H2O4 requires: C, δ3.69; H, 4.96; N, 12.61%). δ H (360MHz, DMSO-de) 2.64-3.20 (16H, m), δ.04 (IH, dm, J=50Hz), 7.02-7.16 (3H, m), 7.38-7.40 (3H, m), 7.51 (IH, d, 8.8Hz), 7.83 (IH, s), 9.01 (2H, s), 0 11.25 (IH, s); m/e (ES) 4δl (M + +l). EXAMPLE 3 4-Benzyl-l-f(R.SV2-fluoro-3-r5-(1.2.4-trιazol-4-yl)-lH-indol-3- lδ yllpropyljpiperidine. 1.75 Hvdrogen Oxalate To a stirred solution of Intermediate 3 (200mg, 0.77mmol) in anhydrous tetrahydrofuran (30ml) was added anhydrous triethylamine (214μl, 1.54mmol) followed by methanesulphonyl chloride (121μl, 1.54mmol). After being stirred for 2h 50 minutes at room temperature, 20 under nitrogen, the mixture was diluted with ethyl acetate (125ml), washed with brme-water (1:1, 25ml), brine (25ml), then dried (MgSO 4 ) and concentrated. A mixture of the mesylate thus obtamed, anhydrous potassium carbonate (128mg) and 4-benzylpιpendιne (270μl) in isopropanol (60ml) 25 was refluxed, under nitrogen, for 18.5 hours. Additional 4- benzylpipendine (3ml) was added and refluxing was resumed for 24 hours Solvents were removed under vacuum and the residue was partitioned between water (60ml) and ethyl acetate (75ml). The organic phase was washed with brme (50ml), dried (MgS0 ) and concentrated. Flash 30 chromatography of the residue (silica gel, dichloromethane-methanol, 90: 10; and silica gel. dichloromethane-methanol, 92:8) afforded 173mg (δ4%) of the title compound free base. The oxalate salt was prepared from ethanol-diethyl ether, mp 9δ-100°C. (Found: C, δ9.53; H, δ.43; N, 11.99. C25H28FN5 x 1.7δC 2 H 2 O 4 requires: C, δ9.δ2; H, δ.52; N, 12.18%). δ H (360MHz, DMSO-d G ) 1.34-l.δ2 (2H, m), 1.64-1.80 (3H, ), 2.74-2.94 (2H, δ m), 3.06-3.44 (6H, m), δ.20-5.42 (IH, dm, J=47Hz), 7.12-7.22 (3H, m), 7.24- 7.40 (4H, m), 7.δ2 (IH, d, J=8.5Hz), 7.84 (IH, d, J=1.9Hz), 9.00 (2H, s), 11.33 (IH, s); m/e (ES) 418 (M + +1). EXAMPLE 4 0 4-r(R.S)-2-(2-Fluorophenyl)propyll-l-{(R.S)-2-fluoro-3-[5-(L2.4-trιazoI-4-vD- lH-mdol-3-yllpropyllpιperazιne Methyl 2-fluorophenylacetate (24.2 g, 0.144 mol) in THF (75 ml) was added dropwise over 20 min to a -78°C solution of LDA 5 (diisopropylamine (16.0 g, 0.1 δ8 mol) and n-butyl lithium (2.5 M, 61 ml)) in THF (300 ml) and stirring was continued for lh. Methyl iodide (22.5 g, 0.158 mol) was added dropwise over 15 min and the reaction mixture allowed to warm to room temperature over lh. The solution was poured mto saturated ammonium chloride solution and extracted with diethyl 0 ether. The extract was washed with brme, dried and evaporated to yield methyl 2-(2-fluorophenyl)propanoate (26.2 g, 100%) as an oil. Methyl 2-(2-fluorophenyl)propanoate (26.2 g, 0.144 mol) was dissolved in THF (325 ml) and LiOH (1 M, 173 ml) added The reaction mixture was stirred at room temperature for 2.5h, before diluting with 5 diethyl ether and extracting with water and IN NaOH The aqueous layer was acidified and extracted with ethyl acetate (x2). The organic extract was washed with brine, dried and evaporated to yield 2-(2- fluorophenyl)propanoιc acid (22.5 g, 100%) as an oil. 2-(2-Fluorophenyl)propanoιc acid (7 7 g, 0 046 mol), 1- 0 benzylpiperazine (8.0 g, 0 045 mol), triethylamine (4.6 g, 0 046 mol) and 1 (3-dιmethvlamιnopropyl)-3-ethvl carbodiimide hydrochlonde (9.6 g, 0.050 mol) were dissolved in dichloromethane (150 ml) and stirred at room temperature for 18h. The solution was evaporated and the residue diluted with water and extracted with brine, dried and evaporated. Chromatography on sihca gel with dichloromethane-methanol (97:3) yielded 4-benzyl-l-[2-(2-fluorophenyl)propanoyl]piperazιne (δ.δ4 g, 38%) as an oil. Lithium aluminium hydride (IM, 34 ml) was added dropwise over 15 min to a 0°C solution of 4-benzyl-l-[2-(2- fluorophenyl)propanoyl]piperazιne (5.δ4 g, 0.017 mol) in THF (lδO ml). The reaction mixture was stirred at 0°C for 10 mm and at room temperature for 24h, cooled to 0°C and quenched with water (1.6 ml), 4N NaOH (1.6 ml) and water (4.5 ml). The solid was filtered off through celite and the filtrate evaporated. Chromatography on sihca gel with dichloromethane-methanol (99:1) yielded 4-benzyl-l-[2-(2- fluorophenyl)propyl]piperazιne (3.17 g, 60%) as a colourless oil. 4-Benzyl-l-[2-(2-fluorophenyl)propyl]piperazιne (3.17 g, 0.01 mol), ammonium formate (3.2 g, O.Oδ mol) and palladium (II) hydroxide on carbon (10% w/w, 1.6 g) were mixed in methanol (200 ml) and heated under reflux for 18h. The catalyst was filtered off through celite and the filtrate evaporated. Chromatography on silica gel with dichloromethane- methanol-880 ammonia (80:8:1) yielded l-[2-(2- fluorophenyl)propyljpιperazιne (1.64g, 73%) as a colourless oil, δH (2δOMHz, CDCh) 1.2 (3H, d, J 7.0, Me), 2.3-2.6 (6H, m, CH 2 ), 2.8-3.0 (4H, m, CH 2 ), 3.1-3.2 (IH, m, MeCH), 6.9-7.4 (4H, m, ArH). δ The mesylate (0.δ77 mmol) from Example 3, l-[2-(2- fluorophenyl)propyl]pιperazme (1.0 g, 4.δ mol), potassium carbonate (0.096 g, 0.692 mmol) and sodium iodide (0.08δ g, 0.577 mmol) were dissolved in isopropyl alcohol (30 ml) and heated under reflux for 66h. The solvent was evaporated, and the residue diluted with water and extracted 0 mto ethyl acetate (x2). The extract was washed with brme, dried and evaporated. Chromatography on silica gel with dichloromethane-methanol (94:6) yielded the title compound (0.126 g, 47%) which was characterised as its oxalate salt; white crystals, mp 185-186°C (from methanol/diethyl ether); (Found C, 55.81; H, 5.75, N, 11.85 C26H30N6F2 1 75C 2 H 2 O 4 . O.8H2O requires C, δ5.66, H, 5.56, N, 13.20%), δ H (360MHz, de-DMSO) 1.2 j (3H, d, J 7, Me), 2 7-3.1 (13H, m, CH ), 3.3-3.4 (2H, m, CH 2 ), 5.0-5 2 (IH, dm, Jm δ0, CHF), 7.0-7.4 (6Η, m, ArH), 7.7 (IH, d, J 9, indole H-7), 7.8 (IH, s, indole H-4), 9 0 (2H, s, tnazole-H), 11.2 (IH, br s, NH); m/z (ES) 46δ (M + +H) The following Examples were made in the same way: 0 EXAMPLE 5 4-r(R,S)-2-(3-Fluorophenyl)propyll-l-((R.S)-2-fluoro-3-fδ-(1.2.4-trιazol-4-yl)- lH-mdol-3-yllpropylVpιperazιne lδ Oxalate salt, white crystals, mp 183-18δ°C (from methanol-diethyl ether), (Found C, 53 70, H, 5 6δ; N, 11 67 C26H30N6F2 2.0 C 2 H 2 O 4 1 δH 2 O requires C, δ3 65, H, 5 55, N, 12.51%), δ H (360MHz, de-DMSO) 1 2 (3H, d, J 7, Me) 2 7-3 1 (15H, m, CH 2 ), 5 0-5 2 (IH, dm, JHF 50, CHF), 7.0- 7 4 (6Η, m ArH), 7 5 (IH, d, J 9, mdole H-7), 7 8 (IH, s, indole H-4), 9 0 20 (2H, s, tnazole-H), 11 2 (IH, br s, NH), m/z (ES) 465 (M + +H) EXAMPLE 6 4-[(R.S -2-(4-Fluorophenyl)propyll-l-{(R,S -2-fluoro-3-f5-(1.2.4-trιazol-4-yl)- 25 lH-ιndol-3-yllpropyllpιperazιne Oxalate salt, white crystals, mp 187-189°C (from methanol-diethyl ether), (Found C, 54 96, H, 5 72, N 11 75 C2GH 3 ONGF2 1 75 C 2 H 2 0 4 1 2H 2 O requires C, 55 04, H, 5.62, N, 13 06%), δ H (360MHz, de-DMSO) 1 (3H, d, J l, Me) 2 7-3 1 (15H, m CH 2 ), 5 0-5 2 (IH, dm, JHΓ 45, CHF) 7 15 30 (2H, apparent t J 9 ArH), 7 25-7 4 (4H m, ArH) 7 5 (IH, d. J 9, mdole H- 7), 7.8 (IH, s, indole H-4), 9.0 (2H, s, triazole-H), 11.3 (IH, br s, NH), m/z (ES) 465 (M + +H). EXAMPLE 7 4-(2-Phenylethyl -l-((R.S)-2-fluoro-3-lδ- ( 1.2.4-triazol-4-vI)-lH-indol-3- yllpropyljpiperazine Oxalate salt; mp 194-196°C (from methanol-diethyl ether); (Found: C, δδ.87; H, δ.92; N, 12.23. C25H29N6F2. 2.0 C 2 H 2 O 4 . 0.7H 2 O requires C, 55.71; H, 5.5δ; N, 13.44%); δ H (360MHz, de-DMSO) 2.8-3.3 (16H, m, CH 2 ), 4.9-5.2 (IH, dm, JHF 50, CHF), 7.2-7.4 (7Η, m, ArH), 7.5 (IH, d, J 9, indole H-7), 7.8 (IH, s, indole H-4), 9.0 (2H, s, triazole-H), 11.2 (IH, br s, NH), m/z (ES) 433 (M + +H). EXAMPLE 8 4-r(R.SV2-Phenylpropyll-l-{(R.S)-2-fluoro-3-r5-ri,2,4-tnazol-4-yl)-lH-indol- 3-vUpropylipiperazine Oxalate salt; white crystals (from methanol-diethyl ether); (Found: C, 5δ.80; H, δ.86; N, 12.06. C26H31N6F2. 2.0C 2 H 2 O 4 . 1.0H 2 O requires C, δδ.86; H, δ.79; N, 13.04%); δ H (360MHz, dβ-DMSO) 1.2 (3H, d, J l, CH ) 2.7-3.2 (15H, m, CH2), 5.0-5.2 (1Η, dm, JΗF 50, CHF), 7.1-7.4 (7Η, m, ArH), 7.5 (IH, d, J 9, mdole H-7), 7.8 (IH, s, mdole H-4), 9.0 (2H, s, triazole-H), 11.2 (IH, br s, NH), m/z (ES) 447 (M + +H). EXAMPLE 9 4-f2-(2-Fluorophenyl)ethyll-l-{(R.S)-2-fluoro-3- 5-(l,2,4-trιazol-4-yl -lH- indol-3-yllpropyllpiperazιne Oxalate salt; white crystals, mp 197-210°C (from EtOH); (Found: C. 54.68; H, 5.21; N, 13.21. C 2 fiH 2 8F 2 N (; . 2C2H2O ,. 0.2H 2 O requires C, 54.46: H, 5.20; N, 13.14%); δ H (360MHz, de-DMSO) 2.7-3.2 (16H, m, CH 2 ), 5.0-5.1 (IH, dm, JH F 48, CHF), 7.1 (2Η, m, ArH), 7.3-7.4 (4H, m, ArH), 7.52 (IH, d, J 8.6, ArH), 7.84 (IH, d, J 1.9, ArH), 9.02 (2H, s, tnazole-H), 11.27 (IH, br s, NH), m/z (ES) 451 (M + +H). EXAMPLE 10 4-f2-(4-Fluorophenyl)ethyll-l-{(R.S)-2-fluoro-3-f5-(L2,4-trιazol-4-yl)-lH- ιndol-3-yllpropyl}piperazιne Oxalate salt; white crystals, mp 188-190°C (from EtOH); (Found: C, 54.22; H, 5.03; N, 12.74. C25H28F 2 N 6 . 2C 2 H 2 O 4 . 0.2H 2 O requires C, 54.46; H, 5.20; N, 13.14%); δ H (360MHz, de-DMSO) 2.8-3.2 (16H, m. CH 2 ), 5.0-5.1 (IH, dm, JHF 50, CHF), 7.15 (2H, t, 9, ArH), 7.2-7.3 (4H, m, ArH), 7.51 (IH, d, J 8.6, ArH), 7.83 (IH, d, J 1.9, ArH), 9.01 (2H, s, tnazole-H), 11.26 (IH, br s, NH), m/z (ES) 4δl (M + +H). EXAMPLE 11 4-r(R,S)-2-(3-Pyridyl)propyll-l-((R.S)-2-fluoro-3-f5-(1.2.4-trιazol-4-yl)-lH- ιndoI-3-yllpropyl|pιperazme Ethyl 3-pyrιdylacetate (12.2 g, 73.9 mmol) in THF (30 ml) was added to LDA (81 mmol) in THF (150 ml) over 10 mm at -78°C. After lh Mel (5.1 ml, 81 mmol) was added to the yellow suspension and the mixture brought to room temperature. H2O (300 ml) and ether (300 ml) was added, the mixture separated, and the organic layer washed with H 2 0 and brine, dried and evaporated to give ethyl 2-(3-pyrιdyl)propanoate (8 3 g) as a yellow oil. This was dissolved m THF (150 ml) and LiAlH 4 (l in THF, 47 ml) added at 0°C. After lh H2O (1.8 ml) was added, followed by 4M NaOH (1.8 ml) and H 2 0 (5.6 ml) After stirring for lh the mixture was filtered and evaporated to give 2-(3-pyrιdyl)ρropan-l-ol (6.3 g) as a yellow oil Methanesulfonyl chloride (2.85 ml, 36.1 mmol) was added to this alcohol (4.5 g, 32.8 mmol) and Et 3 N (δ ml) in THF (70 ml) at 0°C. After lh saturated NaHCO3 was added and the mixture extracted with EtOAc (x3). The organic layers was washed with brine, dried and evaporated. K 2 CO 3 δ (δ g), 'PrOH (60 ml) and BOC-piperazine (12.2 g, 65.5 mmol) were added and the mixture refluxed for 24h. After cooling, the solvent was evaporated, EtOAc (lδO ml) added, and the mixture washed with H 2 O, brine, dried, evaporated, and punfied by flash chromatography, eluting with CH 2 Cl 2 :MeOH (9δ:δ v/v) to give l-tert-butyloxycarbonyl-4-[(R,S)-2-(3- 0 pyndyl)propyl]pιperazιne (3.δl g) as a white sohd. This was dissolved in MeOH (δO ml) and HCl gas bubbled through for 5 mm. After standing for a further 15 minutes the solvent was evaporated, and the residue purified by ion exchange chromatography on DOWEX 50Wx8-200 (H + form) resm, washing with H 2 O and elutmg the lδ product with 3% NH 3 in H 2 O:MeOH (1:1 v/v) to give l-[(R,S)-2-(3- pyrιdyl)propyl]pιperazιne (1.89 g) as a light brown oil. This was coupled to the mesylate of Example 3 to give the title compound: oxalate salt; white crystals (from ethanol-diethyl ether); (Found: C, δ3.δ9, H, 5.46; N, 15.23. C 25 H3θN 7 F 2.0C 2 H 2 O 4 . I.IH2O 20 requires C, 53 79; H, 5.64; N, 15.14%); δ H (360MHz, de-DMSO) 1.2 (3H, d, J 7, Me), 2.7-3 2 (15H, m, CH 2 ), 5.0-5.2 (IH, dm, JHF 50, CHF), 7.3 (3Η, m, ArH), 7.5 (IH, d, J 9, mdole H-7), 7 7 (IH, d, J 8, pyridine H-4), 7 8 (IH, s, indole H-4), 8.4 (IH, d, J 4, pyndine H-2), 8.5 (IH, s, pyndine H-6), 9.0 (2H, s, tnazole-H), 11.2 (IH, br s, NH), m/z (ES) 448 (M + +H). 25 EXAMPLE 12 4-r(R.S)-2-(4-Pyrιdyl)propyll-l-{(R,S)-2-fluoro-3-[5-(l,2.4-trιazol-4-yl)-lH- rndol-3-vI)propy pιperazιne 30 2-(4-Pyrιdyl)propan-l-ol was prepared by the literature procedure (J Indian Chem. Soc. (1966), 43, 549) and taken through as in Example 1 1 to give the title compound: oxalate salt; white crystals (from ethanol- diethyl ether); (Found: C, 54.05; H, 5.41; N, 14.90. C25H30N7F. 2.0C 2 H 2 O 4 . 1.1H 2 0 requires C, 53.79; H, 5.64; N, 15.14%); δ H (360MHz, de-DMSO) 1.2 (3H, d, J 7, Me), 2.7-3.2 (15H, m, CH 2 ), δ.0-δ.2 (IH, dm, JHF δ0, CHF), 7.3 5 (4Η, m, ArH), 7.5 (IH, d, J 9, indole H-4), 8.δ (2H, d, J 7, pyridine H-2), 9.0 (2H, s, triazole-H), 11.2 (IH, br s, NH), m/z (ES) 448 (M + +H). EXAMPLE 13 0 4-r(R.S)-2-(2-Pyrιdyl)propyll-l-((R.S)-2-fluoro-3-rδ-(1.2.4-trιazol-4-yl)-lH- indol-3-yllpropylhoiperazιne 2-(2-Pyridyl)propan-l-ol was prepared by the literature procedure (J. Indian Chem. Soc. (1966) 43, δ49) and taken through as in Example 11 to give the title compound: oxalate salt; white crystals, mp 140-144°C lδ (from EtOH); (Found: C, 53.96; H, 5.34; N, 14.83. C25H30FN6. 2C 2 H 2 O 4 . H 2 O requires C, δ3.9δ; H, 5.62; N, 15.19%); δ H (360MHz, de-DMSO) 1.23 (3H, d, J 6.8, Me), 2.7-3.4 (15H, m, CH 2 and CH), δ.0-δ.l (IH, dm, JHF 50, CHF), 7.2-7.4 (4Η, m, ArH), 7.52 (IH, d, J 8.6, ArH), 7.76 (IH, d, J 2 and 8, ArH), 7.83 (IH, d, J, 2, ArH), 8.50-8.5δ (IH, m, ArH), 9.01 (2H. s, 20 triazole-H), 11.28 (IH, s, NH), m/z (ES) 448 (M + +H). EXAMPLE 14 4-r2-(3-Fluorophenyl)ethyll-l-{(S)-2-fluoro-3-15-(1.2.4-trιazol-4-yl)-lH- 25 indol-3-yllpropyIlpiρerazine Butyllithium (48 ml, 1.6 M) was added to trie thylsily lace tylene (10.7 g, 76.4 mmol) in THF (90 ml) at -78°C over 20 mm. After 10 mm BF 3 .OEt2 (10 ml, 82 mmol) was added, then after another 10 mm (-)- benzyl-(R)-glycidyl ether (8.4 g, 51 mmol) in THF (20 ml) was added over 30 10 mm. The mixture was stirred at -78°C for lh then at 0°C for 20 mm. Saturated NH4CI was added and the mixture extracted with EtOAc (x3). The combined organic layers were washed with H 2 O and brine, dried, evaporated, and purified by chromatography to give (R)-5-benzyloxy-4- hydroxy-1-triethylsilyl-l-pentyne (9.4 g, 61%). This acetylene (7.6 g, 25 mmol), 2-iodo-4-(l,2,4-triazol-4-yl)aniline (5.6 g, 19.6 mmol), LiCl (927 mg, 19.7 mmol), Na 2 CO 3 (8.37 g, 79 mmol), and Ph 3 P (1.03 g, 3.93 mmol) were stirred in DMF (200 ml) at r.t. for 2h whilst N 2 gas was bubbled through the mixture. Pd(OAc) 2 (441 mg, 1.97 mmol) was then added, and the reaction heated at 100°C for 20h. The mixture was cooled, filtered and evaporated. H 2 O (δOO ml) was added, and extracted with ethyl acetate (xδ). The combined organic layers were washed with H 2 O, brine, dried, and evaporated. A mixture of EtOH and δM HCl (300 ml, 1:1 v/v) was added, and the solution stood at r.t. for 18h, then poured into saturated K2CO3, and extracted with EtOAc (x3). The organic layers were washed with water and brine, dried, evaporated, and purified by flash chromatography, eluting with CH 2 Cl 2 :MeOH (92:8 v/v) to give (R)-3-(3-benzyloxy-2-hydroxyprop-l-yl)-δ-(l,2,4-tπazol-4-yl)indole (1.81 g, 26%). This was dissolved in THF (100 ml) containing triethylamine (1.43 ml, 10.3 mmol) and cooled to -δ°C. Methanesulfonyl chloride (820 μl. 10.3 mmol) was added and the mixture washed with H 2 0 and brine, dried, and evaporated. DMF (30 ml) and tetrabutylammonium fluoride (14.4 g, δ2 mmol) was added and the reaction stirred at 50°C for 16h then diluted with H 2 0 (200 ml). The mixture was extracted with EtOAc (x3) and the combined organic layers washed with H 2 O and brine, dried, evaporated and purified by flash chromatography, eluting with CH2C :MeOH (90:10 v/v) to give (S)-l-benzyloxy-2-fluoro-3-(5-[l,2,4-trιazol-4-yl]-lH-indol-3- yl)propane (407 mg, 22%) as a colourless foam. Debenzylation as above gave (S)-2-fluoro-3-(5-[l,2,4-trιazol-4-yl]-lH-mdol-3-yl)propanol as a white solid. Comparison with the racemic material by chiral ΗPLC (Chiral pak AS, 15% EtOΗ in hexane, 1 ml/mm, 40°C) showed the compound to have 95% enantiomeric excess. This alcohol was mesylated and coupled to l-[2-(3- fluorophenyl)ethyl]pιperazme as in Example 3 to give the title compound. EXAMPLE 15 δ 4-r2-(3-Fluorophenyl)ethyll-l-((R)-2-fluoro-3-fδ-(1.2,4-trιazol-4-yl)-lH- indol-3-yl propyl|piperazme This was made in the same way as Example 14, but starting with (+)-benzyl-(S)-glycιdyl ether. 0 EXAMPLE 16 4-f2-(3-Fluorophenyl)ethyll-l-(2,2-dιfluoro-3-f5-(1.2,4-trιazol-4-yl)-lH- ιndol-3-yllpropyl}pιperazιne 1.25 Hvdrogen oxalate 1.0 Hydrate lδ a) Ethyl bromodifluoroacetate (δ g, 24.6 mmol) and t-butyl piperazmecarboxylate (4.δ8 g) were mixed and gently heated to melt the solid. The reaction was allowed to cool. Trituration with petroleum ether (Bp 60-80°C) gave 4-(2-bromo-2,2-dιfluoroacetamιdo)-l-(t- 20 butoxycarbonyl)pιperazme as a colourless solid. δH (2δ0MHz, CDClj) 1.47 (9H, s), 3.48-3 δ5 (4H, m), 3.64-3.66 (4H, m) b) A solution of allyltnbutyl tin (4.5 ml) and the product from above (5.0 g, 14.57 mmol) in 100 ml degassed benzene was heated to reflux under 25 a nitrogen atmosphere m the presence of α,α'-azobιsιsobutyronιtrιle (50 mg). After 24 hours, the reaction was concentrated, and chromatographed using petroleum ether and then 20% ethyl acetate-petroleum ether as eluent. 4-(2,2-Difluoropent-4-enamιdo)-l-(t-butoxycarbonyl)pιperazιne was obtained as an oil which crystallised slowly on standing δn (2δOMHz. 30 CDCh) 1.47 (9H, s), 2 83-3.01 (2H. m), 3 44-3.48 (4H, m), 3.59-3.63 (2H, m), 3.67-3.71 (2H, m), 5.24 (IH, s), 5.28-5.31 (IH, d, J=7.5Hz), δ.70-δ.94 (IH, m). c) A solution of the product from above (0.5 g, 1.7 mmol) in 20 ml δ anhydrous THF was treated with borane-THF complex (8.2 ml of a l.OM solution in THF). The reaction was heated to reflux for 18h, cooled and then concentrated. Methanol was added cautiously, the reaction reconcentrated, and methanol added again. Concentration in vacuo gave a clear oil which was dissolved in 10 ml THF and adjusted to pH 9 using 4N 10 sodium hydroxide. Hydrogen peroxide (30% wt solution, 1 ml) was added, and the reaction allowed to stir at room temperature for 2 l Λ hours The orgamc phase was separated and the aqueous phase was extracted with diethyl ether. The combined organic phases were washed with brine, dried (MgSO ) and concentrated. Chromatography using 30% ethyl acetate - lδ petroleum ether eluent gave 4-(2,2-difluoro-δ-hydroxypentan-l-yl)-l-(t- butoxycarbonyl)piperazιne as a clear oil. m/e (ES) 309 (M + +l); δH (250MHz, CDCh) 1.45 (9H, s), 1.70-1.82 (2H, m), 1.96-2.28 (2H, m), 2.54- 2.64 (4H, m), 2.70-2.81 (2H, m), 3.43-3.47 (4H, m), 3 67-3 72 (2H, m) 20 d) A solution of the product from above (1.28 g, 4.2 mmol) and triethylamine (4 ml) m 12 ml anhydrous DMSO was treated with portions of sulphur trioxide-pyridine complex (1.3 g) using occasional cooling an ice-water bath to moderate the exotherm. When addition was complete, the reaction was cooled in an ice-water bath, quenched with water and 25 extracted into ethyl acetate The organic phase was dried (MgSO ), concentrated and chromatographed using 20-50% ethyl acetate-petroleum ether as eluent 4-(2,2-Difluoro-5-pentanal)-l-(t- butoxycarbonyl)pιperazιne was obtained as a clear oil m/e (ES) 307 (M + +l), δ H (250MHz, CDCh) 1.45 (9H, s), 2.13-2 27 (2H, m), 2.34-2 40 (4H, 30 m), 2.50-2.54 (2H, m), 3.39-3 43 (4H, m), 3.49 (2H, br s), 9 81 (I H, s) e) A solution of the product from above (1.32 g, 4.3 mmol) and 4-(l,2,4- triazol-4-yl)phenylhydrazine (0.75 g) in 4% aqueous sulphuric acid was stirred at room temperature for 20 minutes and then heated to reflux for 21% hours. The reaction was cooled, extracted into butanol, and the organic extracts concentrated. Chromatography using methanol- dichloromethane-ammonia (10:89:1) gave l-{2,2-dϋluoro-3-[δ-(l,2,4-triazol- 4-yl)-lH-indol-3-yl]propyl}piperazine as a brown oil. m/e (ES) 347 (M + +l); δ H (2δ0MHz, CDCh + d 4 MeOD) 2.δ2-2.62 (6H, m), 2.85-2.90 (4H, m), 3.35- 3.48 (2H, t, J=17.5Hz), 7.12-7.16 (IH, m), 7.30 (IH, s), 7.49-7.δ2 (IH, d, J=7.δHz), 7.64-7.6δ (IH, d, J=2.5Hz), 8.5 (2H, s). f) A solution of the product from above (80 mg, 0.2 mmol), acetic acid (40 μl) and 3-fluorophenyl acetaldehyde (3δ mg) in 4 ml methanol was stirred for 10 minutes and then treated with sodium cyanoborohydride (15 mg). A further 8 mg of aldehyde was added, and stirring was continued for 30 minutes. The reaction was quenched with 4N sodium hydroxide, concentrated and chromatographed to give the title compound. The oxalate salt was prepared from ethanol-diethyl ether, mp. 142-14δ°C. (Found: C, 55.53; H, 5.66; N, 13.62. C25H27N6F3. 1.25C 2 H 2 O 4 . H 2 0 requires C, 55.13; H, 5.30; N, 14.02). δ H (360MHz, de-DMSO) 2.64-2.90 (6H, m), 2.90-2.95 (2H, m), 3.00-3.22 (6H, m), 3.36-3.50 (2H, d, J=18 Hz), 7.06-7.15 (3H, m), 7.32-7.41 (3H, m), 7.52-7.55 (IH, d, J=llHz), 7.82 (IH, s), 8.99 (2H, s), 11.37 (IH, s); m/e (ES) 469 (M + +l). EXAMPLE 17 4-f(±)-2-Phenylpropyll-l-l2,2-difluoro-3-f5-(1.2,4-trιazolyl-4-yl -lH-ιndol-3- yllpropyllpiperazine 1.0 Hvdrogen Oxalate 0.9 Hydrate 1.3 Ethanolate 2-Phenylpropιonaldehyde (50 mg, 0.38 mmol) was reacted in a similar manner as described in Example 16. step f to give the title compound, mp. softens at 109°C. δH (360MHz. de-DMSO) (Found C. 58.33; H, 6.25; N, 12.93. C26H 3 oN 6 F 2 . C2H2O4. 0.9H 2 O. 1.3C 2 H OH requires C, 58.27; H, 6.65; N, 13.33). δ H (250MHz, d -MeOH, free base) 1.19 (3H, d, J=6.7Hz), 2.30-2.64 (12H, m), 2.84-2.98 (IH, m), 3.42 (2H, t, J=16.2Hz), 7.14-7.31 (7H, m), 7.51-7.65 (IH, m), 7.83 (IH, m), 8.89 (2H, s). m/e (ES) 5 46δ (M + +1). EXAMPLE 18 4-r(±)-2-(4-Fluorophenyl)propyll-l-{2.2-difluoro-3-15-(1.2.4-trιazol-4-yl)-lH- 0 ιndol-3-yllpropyllpιperazιne Hvdrogen Oxalate 2-(4-Fluorophenyl)propιonaldehyde (49 mg, 0 31 mmol) and the piperazine from Example 16, step e (110 mg, 0.31 mmol) were reacted in a similar manner as described in Example 16, step f, to give the title compound, mp. softens at 65°C. δ H (δOOMHz, de-DMSO) 1.14 (3H, d, lδ J=6.9Hz), 2.29-2.49 (10H, m), 2.δ9 (2H, t, lδHz), 2.91 (9H, q, J=5Hz), 3.42 (2H, t, J=15Hz), 7.09 (2H, t, J=δHz), 7 23-7.2δ (2H, m), 7.30-7.32 (IH, dd, J=10 and 5Hz), 7.36-7.37 (IH, d, J=5Hz), 7 δO-7 δl (IH, d, J=δHz), 7.80- 7 81 (IH, d, J=δHz), 8.97 (2H, s), 11 31 (IH, s) m/e (ES) 483 (M + +l). 20 EXAMPLE 19 4-f2-(2-Thιenyl)ethyll-l-{2.2-dιfluoro-3-fδ-(1.2,4-trιazol-4-yl)-lH-ιndol-3- yllpropyllpiperazmc 1.4 Hvdrogen Oxalate 25 a) A solution of 2-(2-thιenyl)ethanol (5 76 g, 45 mmol) and triethylamine (6.89 ml) m 30 ml anhydrous THF was stirred at 0°C under a nitrogen atmosphere. Methanesulfonyl chloride was added dropwise The reaction was stirred for V h, washed with water, and the organic phase dried (MgS0 4 ) and concentrated to give the mesylate δn (250MH/., 30 CDCh) 2.92 (3H, s), 3 27 (2H, t, J=7 5Hz), 4 42 (2H t , J=7 5Hz), 6 90-6 98 (2H, m), 7.18-7.26 (IH, m) b) A solution of the mesylate from above (47 mg), the piperazine from Example 16, step e (80 mg, 0.2 mmol) and potassium carbonate (32 mg) in anhydrous DMF was heated to 100°C for 2 1 /. hours. A further quantity of the mesylate was added after 1 hour. The reaction was partitioned between ethyl acetate and water. The organic phase was dried (MgSO ), concentrated and chromatographed using methanol-dichloromethane- ammonia (5:9δ:l). The salt was prepared as described in Example 16, step f, to give the title compound, mp.l05-107°C (Found: C, 53.17; H, 4.77; N, 14.72. C28H 2 eNeSF2. 1.4C 2 H 2 O 4 requires C, 53.18; H, 4.98; N, 14.42). δ H (360MHz, de-MeOD, free base) 2.49-2.66 (11H, m), 2.95-3.00 (2H, m), 3.11- 3.12 (IH, m), 3.45 (2H, t, J=14.4Hz), 6.84-6.85 (IH, m), 6.88-6.91 (IH, m), 7.16-7.17 (IH, m), 7.26-7.34 (2H, m), 7.52-7.δδ (IH, m), 7.83 (IH, s), 8.90 (2H, s). m/e (ES) 567. EXAMPLE 20 4-r2-(3-Thienvnethyll-l-{2.2-difluoro-3-r5-(1.2.4-triazol-4-yl)-lH-indol-3- yllpropy piperazine 1.75 Hvdrogen Oxalate 1.0 Ethanolate The title compound was prepared in a similar method as described in Example 19 using 2-(3-thienyl)ethanol, mp. 161-164 °C (Found C, 50.94; H, 5.δ3; N, 11. δδ. C 2 3H 2 cF 2 NeS. 1.7δC 2 H 2 O 4 . C 2 H 5 OH requires C, δ4.94; H, 5.16; N, 15.37). δ H (360MHz, de-DMSO) 2.68-2.90 (6H, m) 2.92-3.00 (2H. m), 3.04-3.30 (6H, m), 3.38-3.50 (2H, m), 7.05-7.06 (IH, m), 7.30-7.38 (2H. m), 7.40-7.44 (IH, m), 7.50-7.δ8 (2H, m), 7.80-7.84 (IH, m), 9.00 (2H, s), 11.37 (IH, s). m/e (ES) 4δ7 (M + +l). EXAMPLE 21 4-f(S)-2-PhenvIpropyll-l-(2.2-difluoro-3-rδ-(1.2,4-triazol-4-yl)-lH-indol-3- yllpropyDpiperazine 2.1 Hvdrogen Oxalate 2.0 Hydrate δ a) A solution of 4-(2,2-difluoropent-4-enamιdo)-l-(t- butoxycarbonyl)piperazine (Example 16, step b) (δ.O g, 16.4 mmol) in 10 ml dichloromethane was treated with 2 ml trifluoroacetic acid. The reaction was concentrated after 24 hours, basified to pH 9 using 4N 0 sodium hydroxide and extracted into dichloromethane. The organic extracts were dried (MgSO ) and concentrated to give 4-(2,2-difluoropent- 4-enamιdo)pιperazιne as a yellow oil. δ H (250MHz, CDCh) 2.83-3.01 (6H, m), 3.59-3.63 (2H, t, J=10Hz), 3.67-3.71 (2H, t, J=10Hz), 5.23 (IH, s), 5.27- 5.30 (IH, d, J=7.5Hz), δ.79-δ.9δ (IH, m). lδ b) A solution of the product from above (1.49 g, 7.3 mmol), triethylamine (2 ml) and (S)-(+)-2-phenylpropιonιc acid (1.1 g, 7.3 mmol) in 10 ml anhydrous dichloromethane was stirred at 0°C under a nitrogen atmosphere and was treated with bιs(2-oxo-3-oxazolιdmyl)phosphinιc 20 chlo de (1.95 g, 7.7 mmol). The reaction was stirred at 0°C for %h and then at room temperature for lh. The reaction was washed with water, dried (MgSO 4 ), concentrated and chromatographed using 20→δ0% ethyl acetate-petroleum ether eluent. 4-(2,2-Difluoropent-4-enamιdo)-l-[(S)-2- phenylpropιonamιdo]piperazιne was obtained as a clear oil. δπ (250MHz, 25 CDCh) 1.44-1.47 (3H. d, J=7.5Hz), 2.78-2.95 (3H, m), 3.2-3.5 (4H, m), 3.52- 3.70 (IH, m), 3.70-4.60 (3H, m), 5.20-5.27 (2H, m), 5.73-5.89 (IH, m), 7.21 - 7.36 (5H, m). c) The bis-amide from above (1.0 g, 3 mmol) was reacted in a similar 30 manner as described in Example 16, step c. 4-(2.2-Difluoro-5-hydroxy- pentan-l-yl)- l-[(S)-2-phenylpropyl]pιperazιne was obtained as a clear oil. δ H (2δOMHz, de-DMSO) 1.28-1.30 (3H, d, J=δHz), 1.60-1.74 (2H, m), 1.90- 2.18 (2H, m), 2.4-2.7 (10H, m), 2.79 (2H, t, J=15Hz), 3.0-3.1 (IH, m), 3.48- 3.δ8 (2H, m), 4.64 (IH, t, J=6Hz), 7.29-7.40 (5H, m); m/e (ES) 327 (M + +l). d) The product from above (326 mg, 1 mmol) was reacted in a similar manner as described in Example 16, step d, and was used without purification in the next step. e) The product from above was reacted in a similar manner as described in Example 16, step e, to give the title compound. The salt was prepared as described in Example 16, step f. mp. 127-130°C. (Found: C, δ2.δ7; H, 5.13; N, 12.15. C 26 H3θN 6 F2. 2.1C 2 H 2 O 4 . 2H 2 O requires C, 52.59; H, 5.δ8; N, 12.18). δ H (250MHz, d 4 -MeOD, free base), 1.20 (3H, d, J=2.5Hz), 2.36-2.54 (9H, m), 2.48 (2H, t, J=10Hz), 2.87-2.96 (2H, m), 3.34 (2H, t, J=17.5Hz), 7.14-7.32 (7H, m), 7.51-7.54 (IH, m), 7.83 (IH, d, J=2.5Hz), 8.88 (2H, s). m/e (ES) 46δ (M + +l). EXAMPLE 22 4-[(+)-2-(3-Pyrιdyl)propyll-l-(2.2-difluoro-3-rδ-(1.2,4-trιazol-4-yl)-lH-ιndoI- 3-yllpropylipιperazme 3.5 Hvdrogen Oxalate 0.12 Hydrate a) A solution of hexamethylene tetramine (12 g) and 5-(l,2,4-trιazol-4- yl)-lH-indole (10.5 g, 57.1 mmol) in acetic acid (125 ml, 30% v/v) was heated at reflux for 3 hours. The reaction was neutralized with potassium carbonate and the water removed in vacuo. The residue was triturated with water and the solid collected to give 5-(l,2,4-tnazol-4-yl)-3- (carboxaldehyde)-lH-ιndole as a brown solid, m/e (ES) 213 (M + +l). b) A suspension of the product from above (1.01 g, 4.8 mmol), N.N- dimethylammopyπdine (DMAP) (47 mg) and di- ri-butyldicarbonate (1.03 g) in 2δ ml dichloromethane, was stirred for 8 hours. Further quantities of DMAP (δO mg) and di-tert-butyldicarbonate (200 mg) were added after 1.5 hours. The reaction was concentrated, and the solid triturated with methanol, to give 5-(l,2,4-triazol-4-yl)-3-(carboxaldehyde)-l-(£ert- δ butoxycarbonyl)indole as a beige solid. δH (2δOMHz, de-DMSO) 1.90 (9H, s), 7.96-8.00 (IH, m), 8.46 (IH, d, J=10Hz), 8.δ4 (IH, d, J=3Hz), 9.03 (IH, s), 9.38 (2H, s), 10.34 (IH, s). m/e (ES) 313 (M + +l). c) A suspension of activated zinc dust (85 mg) and the aldehyde from 0 above (3.2 mg, 1 mmol) in 3 ml THF and 5 drops DMF was heated to reflux under a nitrogen atmosphere. Ethyl bromodifluoroacetate (0.14 ml, 1.1 mmol) was added. After lδ minutes, a further O.δ eq. of ethyl bromodifluoroacetate was added, followed after lδ minutes by phenyl chlorothionoformate (0.18 ml, 1.3 mmol). The reaction was heated at 5 reflux 1.5 hours, and partitioned between water-ethyl acetate. The combined organic phases were separated, dried (MgSO ), concentrated and chromatographed using 5% methanol-dichlorome thane. The partially purified product was heated to reflux in lδ ml degassed toluene with tributyl tin hydride (0.27 ml, 1 mmol) and α, '-azobisιsobutyronιtrile (118 0 mg). After 2.5 hours, the reaction was concentrated, and chromatographed using 2->5% methanol-dichloromethane. Ethyl 2,2-difluoro-3-[5-(l,2,4- triazol-4-yl)-l-(tert-butoxycarbonyl)indol-3-yl]propanoate was obtained as a yellow oil. m/e (ES) 421 (M + +l). δ H (2δ0MHz. CDCh) 0.92 (3H, t, J=7.δHz), 1.69 (9H, s), 3.49 (2H, t, J=15Hz), 4.28 (2H, q, J=7.5Hz), 7.30- δ 7.3δ (IH, m), 7.56 (IH, m), 7.67 (IH, s), 8.30-8.34 (IH, m), 8.50 (2H, s). d) A solution of the product from above (179 mg, 0.4 mmol) in 3ml ethanol was treated with sodium borohydride (20 mg), and the reaction monitored by tic. When all the starting material had reacted, the reaction 30 was quenched by addition of water. The ethanol was removed in vacuo, and the products extracted with ethyl acetate. The organic phase was dried (MgSO 4 ), concentrated and chromatographed using 5% methanol- dichloromethane as eluent. 2,2-Difluoro-3-[δ-(l,2,4-trιazol-4-yl)-l-(tert- butoxycarbonyl)indol-3-yl]propan-l-ol was obtained as a yellow oil. m/e 379 (ES) (M + +l). δ H (250MHz, CDCh) 1.69 (9H, s), 3.42 (2H, t, J=17.5Hz), δ 3.77 (2H, t, J=12.5Hz), 7.30-7.34 (IH, m), 7.69 (IH, s), 7.74 (IH, s), 8.29- 8.32 (IH, m), 8.60 (2H, br s). e) A solution of the product from above (80 mg, 0.21 mmol) in 10 ml anhydrous dichloromethane and pyndine (70 μl) was cooled to an internal 10 temperature of -δ0°C under a nitrogen atmosphere. Trifluoromethanesulfonic anhydride (71 μl) was added, and the reaction allowed to stir at -25->-50°C for 1.5h. Five ml of water was added, and the reaction allowed to attain room temperature. The organic phase was separated, dried (MgSO ), and concentrated. The crude triflate was lδ dissolved m 3 ml anhydrous DMF and heated at 120°C for 10 minutes with potassium carbonate (58 mg) and l-[2-(3-pyndyl)propyl]pιperazme (92 mg, 0 45 mmol). The reaction was partitioned between ethyl acetate and water. The organic phase was separated, dried (MgSO- , and concentrated, and chromatographed using dichloromethane -methanol- 20 ammonia (90: 10:1) to give the title compound. (Found C, 49.01, H, 5.13, N 12.51. C 25 H 29 F 2 N7. 3 1 /.C 2 H 2 0 4 . 1/8H 2 0 requires C, 58 37, H, 5.62, N, 17.65 δ H (250MHz. de-DMSO) 1.25-1.28 (3H, m), 2.35-2.62 (12H, m), 2.9-3 0 (IH. m), 3.41 (2H, t, J=17.5Hz), 7.14-7.36 (3H, m), 7.47-7 50 (2H, m). 7 68 (IH, s), 8.47 (4H, m). 25

Claims: CLAIMS: 1. A compound of formula I, or a salt or prodrug thereof: wherein Z represents hydrogen, halogen, cyano, nitro, trifluoromethyl, -OR 5 , -OCOR 5 , -OCONR 5 R G , -OCH 2 CN, -OCH 2 CONR 5 Rc, -SR 5 , -SOR 5 , -SO 2 R5, -SO 2 NR 5 R 6 , -NR 5 R G , -NR 5 COR 6 , -NR 5 CO 2 R 6 , -NR 5 SO 2 R 6 , -COR 5 , -CO 2 R 5 , -CONR 5 R 6 , or a group of formula (Za), (Zb), (Zc) or (Zd). (Za) (Zb) (Zc) (Zd) in which the asterisk * denotes a chiral centre; or Z represents an optionally substituted five-membered heteroaromatic ring selected from furan, thiophene. pyrrole, oxazole, thiazole, isoxazole, isothiazole, imidazole, pyrazole, oxadiazole, thiadiazole, tnazole and tetrazole; X represents oxygen, sulphur. -NH- or methylene, Y represents oxygen or sulphur, E represents a chemical bond or a straight or branched alkvlene chain containing from 1 to 4 carbon atoms, Q represents a straight or branched alkylene chain containing from 1 to 6 carbon atoms, substituted in any position by one or more fluoπne atoms; T represents nitrogen or CH; U represents nitrogen or C-R 2 ; V represents oxygen, sulphur or N-R 3 , -F-G- represents -CH 2 -N-, -CH2-CH- or -CH=C-; R 1 represents C3 6 alkenyl, C 3 6 alkynyl, aryl(d β)alkyl or heteroaryl(Cι.6)alkyl, any of which groups may be optionally substituted; R 2 , R 3 and R 4 independently represent hydrogen or Ci e alkyl; and R 5 and R G independently represent hydrogen, Ci c alkyl, trifluoromethyl, phenyl, methylphenyl, or an optionally substituted aryl(Cι-6)alkyl or heteroaryl(Cι-6)alkyl group; or R 5 and R G , when linked through a nitrogen atom, together represent the residue of an optionally substituted azetidine, pyrrolidine, piperidine, morphohne or piperazine ring 2 A compound as claimed in claim 1 wherein Q represents 2- fluoropropylene, 2,2-dιfluoropropylene or 2-fluoromethyl-propylene 3. A compound as claimed in claim 1 represented by formula IIA, and salts and prodrugs thereof. (IIA) wherein m is zero, 1, 2 or 3; p is zero, 1 or 2; Q 1 represents a straight or branched alkylene chain containing from 2 to δ carbon atoms, substituted in any position by one or more fluorine atoms; T represents nitrogen or CH; G 1 represents nitrogen or CH; A represents nitrogen or CH; B represents nitrogen or C-R 8 ; R 7 and R 8 independently represent hydrogen, Ci-c alkyl, C 2 -e alkenyl, C3-7 cycloalkyl, aryl, aryl(Cι-6)alkyl, C3-7 heterocycloalkyl, heteroaryl, heteroaryl(Cι-e)alkyl, Ci-e alkoxy, Ci-e alkylthio, ammo, CI-G alkylamino, di(Cι-6)alkylamino, halogen, cyano or trifluoromethyl; W represents a group of formula (Wa), (Wb) or (Wc): (Wa) (Wb) (Wc) in which W 1 represents CH or nitrogen; W 2 represents oxygen, sulphur, NH or N-methyl; R 9 represents hydrogen, halogen, cyano. trifluoromethyl, triazolyl, tetrazolyl, Ci e alkyl-tetrazolyl, Ci 1, alkoxy, C 2 e alkylcarbonyl, ammo, Ci e alkylamino, di(Cι.e)alkylamino, di(Cι.e)alkylamιnomethyl, C 2 c alkylcarbonylamino, Ci e alkylsulphonylamino. aminocarbonylamino, Ci e alkylaminocarbonyl, aminosulphonyl or Ci e alkylaminosulphonylmethyl: and R 10 represents hydrogen or C1.3 alkyl. 4. A compound as claimed in claim 1 represented by formula IIB, and salts and prodrugs thereof: (HB) wherein m, p, Q 1 , T, G 1 , W and R 10 are as defined in claim 3; and 0 R 5 and R G are as defined m claim 1. 5. A compound as claimed in claim 1 represented by formula IIC, and salts and prodrugs thereof. lδ (HC) wherein the asterisk * denotes a chiral centre, m, p, Q 1 , T, G 1 , W and R 10 are as defined in claim 3; and R 4 and Y are as defined in claim 1. 20 A compound selected from 4-benzyl-l-[2-fluoromethyl-3-(5-(l,2,4-triazol-4-yl)-lH-indol-3- yl)propyl]piperazine; 4-[2-(3-fluorophenyl)ethyl]-l-[2-fluoro-3-(δ-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]piperazine ; δ 4-benzyl-l-[2-fluoro-3-(δ-(l,2,4-triazol-4-yl)-lH-indol-3- yl)propyl]piperidine; and salts and prodrugs thereof. 7. A compound selected from: 0 4-[2-(2-fluorophenyl)propyl]-l-[2-fluoro-3-(δ-(l,2,4-tnazol-4-yl)-lH-ιndol-3- yl)propyl]piperazine; 4-[2-(3-fluorophenyl)propyl]-l-[2-fluoro-3-(δ-(l,2,4-tnazol-4-yl)-lH-ιndol-3- yl)propyl]piperazine; 4-[2-(4-fluorophenyl)propyl]-l-[2-fluoro-3-(δ-(l,2,4-tnazol-4-yl)-lH-indol-3- lδ yl)propyl]piperazine; 4-(2-phenylethyl)-l-[2-fluoro-3-(δ-(l,2,4-tπazol-4-yl)-lH-indol-3- yl)propyl]piperazine; 4-(2-phenylpropyl)-l-[2-fluoro-3-(δ-(l,2,4-triazol-4-yl)-lH-indol-3- yl)propyl]piperazine; 20 4-[2-(2-fluorophenyl)ethyl]-l-[2-fluoro-3-(δ-(l,2,4-trιazol-4-yl)-lH-mdol-3- yl)propyl]piperazine; 4-[2-(4-fluoiOphenyl)ethyl]-l-[2-fluoro-3-(δ-(l,2.4-trιazol-4-yl)- lH-mdol-3- yl)propyl]pιperazιne; 4-[2-(3-pyrιdyl)propyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lΗ-mdol-3- 25 yl)propyl]pιperazine; 4-[2-(4-pyrιdyl)propyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-ιndol-3- yl)propyl]piperazine; 4-[2-(2-pyridyl)propyl]-l-[2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lΗ-mdol-3- vDpropyljpiperazine; 30 4-[2-(3-fluoiOphenyl)ethyl]-l-[(S)-2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol- 3-yl)propyl]pιperazine; 4-[2-(3-fluorophenyl)ethyl]-l-[(R)-2-fluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-mdol- 3-yl)propyl]piperazine; 4-[2-(3-fluorophenyl)ethyl]-l-[2,2-difluoro-3-(5-(l,2,4-trιazol-4-yl)-lH-indol- 3 -yl)propyl] piperazine ; 5 4-(2-phenylpropyl)-l-[2,2-difluoro-3-(δ-(l,2,4-triazol-4-yl)-lH-indol-3- yl)propyl]piperazine; 4-[2-(4-fluorophenyl)proρyl]-l-[2,2-difluoro-3-(δ-(l,2,4-trιazol-4-yl)-lH- indol-3-yl)propyl]piperazine; 4-[2-(2-thienyl)ethyl]-l-[2,2-difluoro-3-(5-(l,2,4-triazol-4-yl)-lH-indol-3- 10 yl)propyl]piperazine; 4-[2-(3-thienyl)ethyl]-l-[2,2-difluoro-3-(5-(l,2,4-trιazol-4-yI)-lH-indol-3- yl)propyl]piperazine; 4-[(S)-2-phenylpropyl]-l-[2,2-difluoro-3-(δ-(l,2,4-tnazol-4-yl)-lH-indol-3- yl)propyl]piperazine; lδ 4-[2-(3-pyridyl)propyl]-l-[2,2-difluoro-3-(δ-(l,2,4-trιazol-4-yl)-lH-indol-3- yl)propyl]piperazine; and salts and prodrugs thereof. 8. A pharmaceutical composition comprising a compound of 20 formula I as defmed in claim 1 or a pharmaceutically acceptable salt thereof or a prodrug thereof in association with a pharmaceutically acceptable carrier. 9. A compound as claimed in any one of claims 1 to 7 for use in 25 therapy. 10. The use of a compound as claimed in any one of claims 1 to 7 for the manufacture of a medicament for the treatment and/or prevention of clinical conditions for which an agonist of δ-ΗTm receptors selective for 30 the 5-ΗTiD n subtype thereof is indicated. 11. A process for the preparation of a compound as claimed in claim 1, which comprises: (A) attachment of the R 1 moiety to a compound of formula III: wherein Z, E, Q, T, U and V are as defmed in claim 1; or 0 (B) reacting a compound of formula IV: (IV) wherein Z and E are as defined in claim 1; with a compound of formula IX, lδ or a carbonyl-protected form thereof. O / \ Q - N G - R' R- (IX) wherein Q, F, G, R 1 and R 2 are as defmed in claim 1; followed, where 20 required, by N-alkylation by standard methods to introduce the moietv R^ or (C) reacting a compound of formula XI: / \ H- N G-R (XI) wherein F, G and R 1 are as defined in claim 1; with a compound of formula XII: wherein Z, E, Q, T, U and V are as defined in claim 1, and L 2 represents a suitable leaving group; or (D) cyclising a compound of formula XIV: (XIV) m which Z, E, Q, F, G and R 1 are as defined m claim 1, and D 1 represents a readily displaceable group; followed, where required, by N-alkylation by standard methods to introduce the moietv R 3 ; or (E) cyclising a compound of formula XVTII: wherein Z, E, Q, F, G, R 1 and R 2 are as defined in claim 1, and V 1 represents oxygen or sulphur; or (F) reducing a compound of formula XXII: (XXII) wherein Z, E, T, U, V, F, G and R 1 are as defmed in claim 1, and -Q 2 -CH'= corresponds to the moiety Q as defined in claim 1; or (G) reacting a compound of formula XXVI: (XXVI) wherein Z, E, T, U, V, F, G and R 1 arc as defmed in claim 1, and Q 3 corresponds to the moiety Q as defined in claim 1 in which the or each fluorine atom is replaced by a leaving group; with an available source of fluoride ion; and (H) subsequently, if necessary, converting a compound of formula I initially obtained into a further compound of formula I by standard methods. 12. A method for the treatment and or prevention of chnical conditions for which an agonist of δ-HTm receptors selective for the 5-HT α subtype thereof is indicated, which method comprises administering to a patient in need of such treatment an effective amount of a compound of formula I as defined in claim 1 or a pharmaceutically acceptable salt thereof or a prodrug thereof.

Inventors: CASTRO PINEIRO JOSE LUIS, MACLEOD ANGUS MURRAY, ROWLEY MICHAEL, VAN NIEL MONIQUE BODIL, CASTRO PINEIRO, JOSE LUIS, MACLEOD, ANGUS, MURRAY, ROWLEY, MICHAEL, VAN NIEL, MONIQUE, BODIL
Applicants: CASTRO PINEIRO JOSE LUIS, MACLEOD ANGUS MURRAY, MERCK SHARP & DOHME, NIEL MONIQUE BODIL VAN, ROWLEY MICHAEL, CASTRO PINEIRO, JOSE LUIS, MACLEOD, ANGUS, MURRAY, MERCK SHARP & DOHME LIMITED, ROWLEY, MICHAEL, VAN NIEL, MONIQUE, BODIL
Application year: 1996
Main classification: C07D403
IPC codes: C07D521