DETECTION OF HEPATITIS GB VIRUS GENOTYPES

Abstract: HGBV-C oligonucleotides from the 5' end of HGBV-C useful for the detection and genotyping of HGBV-C isolates. Also provided are assays which utilize these oligonucleotides.

Description: DETECTION OF HEPATITIS GB VIRUS GENOTYPES Related Applications This application claims the benefit of U.S. provisional application Serial No. 60/002,265 filed August 14, 1995, which is related to patent application U.S. Serial No. 60/002,255 filed August 14, 1995, which is related to patent applications U.S. Serial No. 08/480,995 filed June 7, 1995, U.S. Serial No. 08/473,475 filed June 7, 1995 and U.S. Serial No. 08/417,629, filed April 6, 1995, which are continuation-in-part applications of U.S. Serial No. 08/424,550 filed June 5, 1995, which is a continuation-in-part application of U.S. Serial No. 08/377,557 filed January 30, 1995, which is a continuation-in-part of U.S. Serial No. 08/344,185 filed November 23, 1994 and U.S. Serial No. 08/344,190 filed November 23, 1994, which are each continuation-in-part applications of 08/283,314 filed July 29, 1994, which is a continuation-in-part application of U.S. Serial No. 08/242,654, filed May 13, 1994, which is a continuation-in-part application of U.S. Serial No. 08/196,030 filed February 14, 1994, all of which enjoy common ownership and each of which is incoφorated herein by reference. Background of the Invention This invention relates generally to hepatitis GB virus and more particularly, relates to oligonucleotide primers and probes useful for detection of genotypes of hepatitis GB virus. Several lines of epidemiological and laboratory evidence have suggested the existence of more than one parenterally transmitted non-A, non-B (NANB) hepatitis-causing agent, including multiple attacks of acute NANBH in intraveneous drug users, distinct incubation periods of patients acquiring NANBH post-transfusion, the outcome of cross-challenge chimpanzee experiments, the ultrastructural liver pathology of infected chimpanzees and the differential resistance of the putative agents to chloroform. J. L. Dienstag, Gastroenterology 85:439-462 (1983); J. L. Dienstag, Gastroenterology 85:743-768 (1983); F. B. Hollinger et al., J. Infect. Dis. 142:400-407"(1980); D. W. Bradley in F. Chisari, ed., Advances in Hepatitis Research. Masson, New York, pp. 268-280 (1984); and D. W. Bradley et al., J. Infect. Pis. 148:254-265 (1983). The detection of hepatitis C virus (HCV) antibody in donor samples now eliminates 70 to 80% of NANBH infected blood in the blood supply system. Thus, the detection of HCV has not totally prevented the transmission of hepatitis. H. Alter et al., New En g . J. Med. 321:1494-1500 (1989). Recent publications also have questioned whether additional hepatitis agents may be responsible for post-transfusion hepatitis (PTH) and for community acquired acute and or chronic hepatitis that is not associated with PTH. For example, of 181 patients monitored in a prospective clinical survey conducted in France from 1988 to 1990, investigators noted a total of 18 cases of PTH. Thirteen of these 18 patients tested negative for anti-HCV antibodies, hepatitis B virus surface antigen (HBsAg), hepatitis B virus (HBV) and HCV nucleic acids. The authors speculated as to the potential importance of a non-A, non-B, non-C agent causing PTH. V. Thiers et al., J. Hepatology 18:34-39 (1993). Also, of 1,476 patients monitored in another study conducted in Germany from 1985 to 1988, 22 cases of documented cases of PTH were not related to infection with HBV or HCV. T. Peters et al., J. Med. NjroL 39:139-145 (1993). Recently, a new family of flaviviruses detected in patients with clinically diagnosed hepatitis was reported. This new family of viruses has been named the "GB" viruses, after the initials of the patient first infected with the virus. These viruses have been reported by J. Ν. Simons et al., Proc. Νatl. Acad. Sci. USA 92:3401-3405 (1995); and J. Ν. Simons et al., Nature Medicine l(6):564-569 (1995). Studies currently are underway to determine the clinical and epidemiological significance of these viruses. As has been noted with hepatitis C virus, genotypes vary in nucleotide and amino acid sequence as well as in severity of the disease and geographical location. See, for example, G. Dawson et al., "Recent Developments in the Molecular Biology of the Hepatitis Virus," in Current Hepatology. G. Gitnick, ed., Mosby Publishers (1995, in press). Thus, detection of genotypes of HGBV can aid in the clinical and epidemiological understanding ofthe virus. The detection of HGBV in test samples can be enhanced by the use of DNA hybridization assays which utilize DNA oligomers as hybridization probes. Since the amount of DNA target nucleotides present in a test sample may be in minute amounts, target DNA usually is amplified and then detected. Methods for amplifying and detecting a target nucleic acid sequence that may be present in a test sample are well-known in the art. Such methods include the polymerase chain reaction (PCR) described in U.S. Patents 4,683,195 and 4,683,202 which are incoφorated herein by reference, the ligase chain reaction (LCR) described in EP- A-320 308 , gap LCR (GLCR) described in European Patent Application EP-A- 439 182 and U.S. Patent No. 5,427,930 which are incoφorated herein by reference, multiplex LCR described in International Patent Application No. WO 93/20227, NASBA and the like. These methods have found widespread application in the medical diagnostic field as well as in the fields of genetics, molecular biology and biochemistry. It would be advantageous to provide DNA probes derived from HGBV which can detect HGBV in test samples of individuals suspected of being infected with HGBV and test kits which utilize these probes. Such probes could greatly enhance the ability ofthe medical community to more accurately diagnose acute and/or chronic viral hepatitis and could provide a safer blood and organ supply by detecting non-A, non-B and non-C hepatitis in these blood and organ donations, and could provide a better understanding of the prevalence of HGBV in the population, epidemiology of the disease caused by HGBV and the prognosis of infected individuals. Summary of the Invention The present invention provides unique primers for HGBV-C detection. These primers are identified as SEQUENCE ID NO 51, SEQUENCE ID NO 53, SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 56, SEQUENCE ID NO 57, and SEQUENCE ID NO 87, and complements thereof. The primer(s) disclosed herein can detect the presence of HGBV-C and are not reactive with HGBV-A or HGBV-B. The present invention also provides a method of detection target HGBV-C nucleotides in a test sample, comprising contacting a target HGBV nucleotide with at least one oligonucleotide and detecting the presence of the target in the test sample. The oiigonucleotides can be selected from the group consisting of SEQUENCE ID NO 51 i SEQUENCE ID NO 53, SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 56, SEQUENCE ID NO 57, and SEQUENCE ID NO 87, and complements thereof. The oiigonucleotides utilized also can be selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, SEQUENCE ID NO 6, SEQUENCE ID NO 9, SEQUENCE ID NO 10, SEQUENCE ID NO 11, SEQUENCE ID NO 13, SEQUENCE ID NO 13, SEQUENCE ID NO 14, SEQUENCE ID NO 15, SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, SEQUENCE ID NO 21, SEQUENCE ID NO 22, SEQUENCE ID NO 23, SEQUENCE ID NO 24, SEQUENCE ID NO 60, SEQUENCE ID NO 61, SEQUENCE ID NO 62, SEQUENCE ID NO 63, SEQUENCE ID NO 64, SEQUENCE ID NO 65, SEQUENCE ID NO 66, SEQUENCEIDNO67,SEQUENCEIDNO68,SEQUENCEIDNO69, SEQUENCEIDNO70,SEQUENCEIDNO71,SEQUENCEIDNO72, SEQUENCEIDNO73,SEQUENCEIDNO74,SEQUENCEIDNO75, SEQUENCEIDNO76,SEQUENCEIDNO77,SEQUENCEIDNO78, SEQUENCEIDNO79,SEQUENCEIDNO80,SEQUENCEIDNO81, SEQUENCEIDNO82,SEQUENCEIDNO83,SEQUENCEIDNO84, SEQUENCEIDNO85andSEQUENCEIDNO86. The present invention also provides a method of amplifying 5' end cDNA of hepatitis GB-C (HGBV-C) virus in a test sample, comprising performing reverse transcription with random primers and test sample, amplifying the cDNA so obtained by using other oligonucleotide primers as sense and antisense primers in a first stage PCR to obtain amplified cDNA of HGBV-C, and detecting the presence of the amplicon (amplified cDNA) in the test sample. At least one oligonucleotide used as a sense primer can be selected from the group consisting of SEQUENCE ID NO. 51, SEQUENCE ID NO 53 andSEQUENCE ID NO 56. At least one oligonucleotide used as an antisense primer can be selected from the group consisting of SEQUENCE ID NO 54, SEQUENCE ID NO 55,SEQUENCE ID NO 57 and SEQUENCE ID NO 87. The present invention also provides a method for detecting target hepatitis GB-C virus (HGBV-C) in a test sample suspected of containing target HGBV-C, comprising contacting the test sample with at least one oligonucleotide of HGBV-C as a sense primer and at least one oligonucleotide of HGBV-C as an anti-sense primer and amplifying same to obtain a first stage reaction product; then contacting the first stage reaction product with at least one ofthe oiigonucleotides used previously and a second oligonucleotide, with the proviso that the second oligonucleotide is located 3' to the first oligonucleotide utilized and is of opposite sense to the first oligonucleotide, and then detecting the HGBV target. The first stage PCR reaction can comprise utilizing at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 51, SEQUENCE ID NO 56 and SEQUENCE ID NO 53 as a sense primer and utilizing at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 57 and SEQUENCE ID NO 87 as an anti-sense primer. The products of this first stage PCR then can be further amplified in a second stage PCR reaction which comprises utilizing at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 56 and SEQUENCE ID NO 53 as a sense primer, and utilizing at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 54, SEQUENCE ID NO 55 and SEQUENCE ID NO 57 as an anti-sense primer, with the proviso that the second oligonucleotide is located 3' to the first oligonucleotide utilized and is of opposite sense to the first oligonucleotide. The amplification in all methods can be performed by the polymerase chain reaction (PCR). The test sample in these methods can be attached to a solid phase prior to performing the methods steps outlined hereinabove. Further, the detection step of these methods can comprise utilizing a detectable measureable signal generating compound Oabel) which generates a measurable signal. Moreover, the label can be attached to a solid phase. GAP LCR also can be performed according to the invention, utilizing SEQUENCE ID NO 35, SEQUENCE ID NO 36, SEQUENCE ID NO 37, SEQUENCE ID NO 38, SEQUENCE ID NO 39, SEQUENCE ID NO 40, SEQUENCE ID NO 41, SEQUENCE ID NO 42, SEQUENCE ID NO 43, SEQUENCE ID NO 44, SEQUENCE ID NO 45, SEQUENCE ID NO 46, SEQUENCE ID NO 47, SEQUENCE ID NO 48, SEQUENCE ID NO 49 and SEQUENCE ID NO 50, for genotype differentiation. Brief Description ofthe Drawings FIGURES 1 A through FIGURE IF show the nucleotide alignment of the HGBV-C isolates. FIGURE 2 shows a phylogenetic tree of the genotypes of HGBV-C. Detailed Description ofthe Invention The present invention provides characterization of a newly ascertained genotypes of etiological agents of non- A, non-B, non-C, non-D and non-E hepatitis-causing agents, collectively so-termed "Hepatitis GB Virus," or "HGBV." The present invention provides a method for detecting HGBV genotypes, oiigonucleotides useful for detecting HGBV and oiigonucleotides useful for differentiating HGBV-C genotypes. The present invention provides kits containing reagents which can be used for the detection of HGBV genotypes, such reagents comprising a polynucleotide probe containing a nucleotide sequence from HGBV of about 8 or more nucleotides in a suitable container The term "Hepatitis GB Virus" or "HGBV", as used herein, collectively denotes a viral species which causes non-A, non-B, non-C, non-D, non-E hepatitis in man, and attenuated strains or defective interfering particles derived therefrom. This may include acute viral hepatitis transmitted by contaminated foodstuffs, drinking water, and the like; hepatitis due to HGBV transmitted via person to person contact (including sexual transmission, respiratory and parenteral routes) or via intraveneous drug use. The methods as described herein will allow the identification of individuals who have acquired HGBV. Individually, the HGBV isolates are specifically referred to as "HGB V-A", "HGB V-B " and "HGBV-C." As described herein, the HGBV genome is comprised of RNA. Analysis of the nucleotide sequence and deduced amino acid sequence of the HGBV reveals that viruses of this group have a genome organization similar to that of the Flaviridae family. Based primarily, but not exclusively, upon similarities in genome organization, the International Committee on the Taxonomy of Viruses has recommended that this family be composed of three genera: Flavivirus, Pestivirus, and the hepatitis C group. Similarity searches at the amino acid level reveal that the hepatitis GB virus subclones have some, albeit low, sequence resemblance to hepatitis C virus. It now has been demonstrated that HGBV-C is not a genotype of HCV. See, for example, U.S. Serial No. 08/417,629, filed April 6, 1995, previously incoφorated herein by reference. The term "similarity" and/or "identity" are used herein to describe the degree of relatedness between two polynucleotides or polypeptide sequences. The techniques for determining amino acid sequence "similarity" and/or "identity" are well-known in the art and include, for example, directly determining the amino acid sequence and comparing it to the sequences provided herein; determining the nucleotide sequence of the genomic material of the putative HGBV (usually via a cDNA intermediate), and determining the amino acid sequence encoded therein, and comparing the corresponding regions. In general, by "identity" is meant the exact match-up of either the nucleotide sequence of HGBV and that of another strain(s) or the amino acid sequence of HGBV and that of another strain(s) at the appropriate place on each genome. Also, in general, by "similarity" is meant the exact match-up of amino acid sequence of HGBV and that of another strain(s) at the appropriate place, where the amino acids are identical or possess similar chemical and/or physical properties such as charge or hydrophobicity. The programs available in the Wisconsin Sequence Analysis Package, Version 8 (available from the Genetics Computer Group, Madison, Wisconsin, 53711), for example, the GAP program, are capable of calculating both the identity and similarity between two polynucleotide or two polypeptide sequences. Other programs for calculating identity and similarity between two sequences are known in the art. Additionally, the following parameters are applicable, either alone or in combination, in identifying a strain of HGBV-A, HGBV-B or HGBV-C. It is expected that the overall nucleotide sequence identity of the genomes between HGBV-A, HGBV-B or HGBV-C and a strain of one of these hepatitis GB viruses will be about 45% or greater, since it is now believed that the HGBV strains may be genetically related, preferably about 60% or greater, and more preferably, about 80% or greater. Also, it is expected that the overall sequence identity of the genomes between HGBV-A and a strain of HGBV-A at the amino acid level will be about 35% or greater since it is now believed that the HGBV strains may be genetically related, preferably about 40% or greater, more preferably, about 60% or greater, and even more preferably, about 80% or greater. In addition, there will be corresponding contiguous sequences of at least about 13 nucleotides, which may be provided in combination of more than one contiguous sequence. Also, it is expected that the overall sequence identity of the genomes between HGBV-B and a strain of HGBV-B at the amino acid level will be about 35% or greater since it is now believed that the HGBV strains may be genetically related, preferably about 40% or greater, more preferably, about 60% or greater, and even more preferably, about 80% or greater. In addition, there will be corresponding contiguous sequences of at least about 13 nucleotides, which may be provided in combination of more than one contiguous sequence. Also, it is expected that the overall sequence identity of the genomes between HGBV-C and a strain of HGBV-C at the amino acid level will be about 35% or greater since it is now believed that the HGBV strains may be genetically related, preferably about 40% or greater, more preferably, about 60% or greater, and even more preferably, about 80% or greater. In addition, there will be corresponding contiguous sequences of at least about 13 nucleotides, which may be provided in combination of more than one contiguous sequence. A polynucleotide "derived from" a designated sequence for example, the HGBV cDNA, or from the HGBV genome, refers to a polynucleotide sequence which is comprised of a sequence of approximately at least about 6 nucleotides, is preferably at least about 8 nucleotides, is more preferably at least about 10-12 nucleotides, and even more preferably is at least about 15-20 nucleotides corresponding, i.e., similar to or complementary to, a region of the designated nucleotide sequence. Preferably, the sequence of the region from which the polynucleotide is derived is similar to or complementary to a sequence which is unique to the HGBV genome. Whether or not a sequence is complementary to or similar to a sequence which is unique to an HGBV genome can be determined by techniques known to those skilled in the art. Comparisons to sequences in databanks, for example, can be used as a method to determine the uniqueness of a designated sequence. Regions from which sequences may be derived include but are not limited to regions encoding specific epitopes, as well as non-translated and/or non-transcribed regions. The derived polynucleotide will not necessarily be derived physically from the nucleotide sequence of HGBV, but may be generated in any manner, including but not limited to chemical synthesis, replication or reverse transcription or transcription, which are based on the information provided by the sequence of bases in the region(s) from which the polynucleotide is derived. In addition, combinations of regions corresponding to that of the designated sequence may be modified in ways known in the art to be consistent with an intended use. The term "polynucleotide" as used herein means a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. This term refers only to the primary structure of the molecule. Thus, the term includes double- and single-stranded DNA, as well as double- and single-stranded RNA. It also includes modifications, either by methylation and/or by capping, and unmodified forms of the polynucleotide. The terms "polynucleotide," "oligomer," "oligonucleotide," "oligo" and "primer" are used interchangeably herein. "HGBV containing a sequence corresponding to a cDNA" means that the HGBV contains a polynucleotide sequence which is similar to or complementary to a sequence in the designated DNA. The degree of similarity or complementarity to the cDNA will be approximately 50% or greater, will preferably be at least about 70%, and even more preferably will be at least about 90%. The sequence which corresponds will be at least about 70 nucleotides, preferably at least about 80 nucleotides, and even more preferably at least about 90 nucleotides in length. The correspondence between the HGBV and the cDNA can be determined by methods known in the art, and include, for example, a direct comparison of the sequenced material with the cDNAs described, or hybridization and digestion with single strand nucleases, followed by size determination of the digested fragments. "Purified viral polynucleotide" refers to an HGBV genome or fragment thereof which is essentially free, i.e., contains less than about 50%, preferably less than about 70%, and even more preferably, less than about 90% of polypeptides with which the viral polynucleotide is naturally associated. Techniques for purifying viral polynucleotides are well known in the art and include, for example, disruption of the particle with a chaotropic agent, and separation of the polynucleotide(s) and polypeptides by ion-exchange chromatography, affinity chromatography, and sedimentation according to density. Thus, "purified viral polypeptide" means an HGBV polypeptide or fragment thereof which is essentially free, that is, contains less than about 50%, preferably less than about 70%, and even more preferably, less than about 90% of cellular components with which the viral polypeptide is naturally associated. Methods for purifying are known to the routineer. "Polypeptide" as used herein indicates a molecular chain of amino acids and does not refer to a specific length of the product. Thus, peptides, oligopeptides, and proteins are included within the definition of polypeptide. This term, however, is not intended to refer to post-expression modifications of the polypeptide, for example, glycosylations, acetylations, phosphorylations and the like. A "polypeptide" or "amino acid sequence derived from a designated nucleic acid sequence or from the HGBV genome refers to a polypeptide having an amino acid sequence identical to that of a polypeptide encoded in the sequence or a portion thereof wherein the portion consists of at least 3 to 5 amino acids, and more preferably at least 8 to 10 amino acids, and even more preferably 15 to 20 amino acids, or which is immunologically identifiable with a polypeptide encoded in the sequence. A "recombinant polypeptide" as used herein means at least a polypeptide of genomic, semisynthetic or synthetic origin which by virtue of its origin or manipulation is not associated with all or a portion of the polypeptide with which it is associated in nature or in the form of a library andor is linked to a polynucleotide other than that to which it is linked in nature. A recombinant or derived polypeptide is not necessarily translated from a designated nucleic acid sequence of HGBV or from an HGBV genome. It also may be generated in any manner, including chemical synthesis or expression of a recombinant expression system, or isolation from mutated HGBV. The term "synthetic peptide" as used herein means a polymeric form of amino acids of any length, which may be chemically synthesized by methods well- known to the routineer. These synthetic peptides are useful in various applications. "Recombinant host cells," "host cells," "cells," "cell lines," "cell cultures," and other such terms denoting microorganisms or higher eucaryotic cell lines cultured as unicellular entities refer to cells which can be, or have been, used as recipients for recombinant vector or other transfer DNA, and include the original progeny of the original cell which has been transfected. As used herein "replicon" means any genetic element, such as a plasmid, a chromosome or a virus, that behaves as an autonomous unit of polynucleotide replication within a cell. That is, it is capable of replication under its own control. A "vector" is a replicon in which another polynucleotide segment is attached, such as to bring about the replication and/or expression of the attached segment. The term "control sequence refers to polynucleotide sequences which are necessary to effect the expression of coding sequences to which they are ligated. The nature of such control sequences differs depending upon the host organism. In prokaryotes, such control sequences generally include promoter, ribosomal binding site and terminators; in eukaryotes, such control sequences generally include promoters, terminators and, in some instances, enhancers. The term "control sequence thus is intended to include at a minimum all components whose presence is necessary for expression, and also may include additional components whose presence is advantageous, for example, leader sequences. "Operably linked" refers to a situation wherein the components described are in a relationship permitting them to function in their intended manner. Thus, for example, a control sequence" operably linked" to a coding sequence is ligated in such a manner that expression of the coding sequence is achieved under conditions compatible with the control sequences. The term "open reading frame" or "ORF" refers to a region of a polynucleotide sequence which encodes a polypeptide; this region may represent a portion of a coding sequence or a total coding sequence. A "coding sequence" is a polynucleotide sequence which is transcribed into mRNA and/or translated into a polypeptide when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a translation start codon at the 5" -terminus and a translation stop codon at the 3' -terminus. A coding sequence can include, but is not limited to, mRNA, cDNA, and recombinant polynucleotide sequences. The term "immunologically identifiable with/as" refers to the presence of epitope(s) and polypeptide(s) which also are present in and are unique to the designated polypeptide(s), usually HGBV proteins. Immunological identity may be determined by antibody binding and/or competition in binding. These techniques are known to the routineer and also are described herein. The uniqueness of an epitope also can be determined by computer searches of known data banks, such as GenBank, for the polynucleotide sequences which encode the epitope, and by amino acid sequence comparisons with other known proteins. As used herein, "epitope" means an antigenic determinant of a polypeptide. Conceivably, an epitope can comprise three amino acids in a spatial conformation which is unique to the epitope. Generally, an epitope consists of at least five such amino acids, and more usually, it consists of at least eight to ten amino acids. Methods of examining spatial conformation are known in the art and include, for example, x-ray crystallography and two-dimensional nuclear magnetic resonance. The term "individual" as used herein refers to vertebrates, particularly members of the mammalian species and includes but is not limited to domestic animals, sports animals, primates and humans; more particularly the term refers to tamarins and humans. A polypeptide is "immunologically reactive" with an antibody when it binds to an antibody due to antibody recognition of a specific epitope contained within the polypeptide. Immunological reactivity may be determined by antibody binding, more particularly by the kinetics of antibody binding, and/or by competition in binding using as competitor(s) a known polypeptide(s) containing an epitope against which the antibody is directed. The methods for determining whether a polypeptide is immunologically reactive with an antibody are known in the art. As used herein, the term "immunogenic polypeptide containing an HGBV epitope" means naturally occurring HGBV polypeptides or fragments thereof, as well as polypeptides prepared by other means, for example, chemical synthesis or the expression of the polypeptide in a recombinant organism. The term "transformation" refers to the insertion of an exogenous polynucleotide into a host cell, irrespective of the method used for the insertion. For example, direct uptake, transduction, or f-mating are included. The exogenous polynucleotide may be maintained as a non-integrated vector, for example, a plasmid, or alternatively, may be integrated into the host genome. "Treatment" refers to prophylaxis and/or therapy. The term "plus strand" (or "+") as used herein denotes a nucleic acid that contains the sequence that encodes the polypeptide. ' The term "minus strand" (or "-") denotes a nucleic acid that contains a sequence that is complementary to that of the "plus" strand. "Positive stranded genome" of a virus denotes that the genome, whether RNA or DNA, is single-stranded and encodes a viral polypeptide(s). The term "test sample" refers to a component of an individual's body which is the source of the analyte (such as, antibodies of interest or antigens of interest). These components are well known in the art. These test samples include biological samples which can be tested by the methods of the present invention described herein and include human and animal body fluids such as whole blood, serum, plasma, cerebrospinal fluid, urine, lymph fluids, and various external secretions of the respiratory, intestinal and genitorurinary tracts, tears, saliva, milk, white blood cells, myelomas and the like; biological fluids such as cell culture supematants; fixed tissue specimens; and fixed cell specimens. "Purified HGBV" refers to a preparation of HGBV which has been isolated from the cellular constituents with which the virus is normally associated, and from other types of viruses which may be present in the infected tissue. The techniques for isolating viruses are known to those skilled in the art and include, for example, centrifugation and affinity chromatography. "PNA" denotes a "peptide nucleic analog" which may be utilized in a procedure such as an assay described herein to determine the presence of a target. PNAs are neutrally charged moieties which can be directed against RNA targets or DNA. PNA probes used in assays in place of, for example, the DNA probes of the present invention, offer advantages not achievable when DNA probes are used. These advantages include manufacturability, large scale labeling, reproducibility, stability, insensitivity to changes in ionic strength and resistance to enzymatic degradation which is present in methods utilizing DNA or RNA. These PNAs can be labeled with such signal generating compounds as fluorescein, radionucleotides, chemiluminescent compounds, and the like. PNAs or other nucleic acid analogs such as moφholino compounds thus can be used in assay methods in place of DNA or RNA. Although assays are described herein utilizing DNA probes, it is within the scope of the routineer that PNAs or moφholino compounds can be substituted for RNA or DNA with appropriate changes if and as needed in assay reagents. "Solid phases" ("solid supports") are known to those in the art and include the walls of wells of a reaction tray, test tubes, polystyrene beads, magnetic beads, nitrocellulose strips, membranes, microparticles such as latex particles, sheep (or other animal) red blood cells, duracytes and others. The "solid phase" is not critical and can be selected by one skilled in the art. Thus, latex particles, microparticles, magnetic or non-magnetic beads, membranes, plastic tubes, walls of microtiter wells, glass or silicon chips, sheep (or other suitable animal's) red blood cells and duracytes are all suitable examples. Suitable methods for immobilizing probes on solid phases include ionic, hydrophobic, covalent interactions and the like. A "solid phase", as used herein, refers to any material which is insoluble, or can be made insoluble by a subsequent reaction. The solid phase can be chosen for its intrinsic ability to attract and immobilize the capture reagent. Alternatively, the solid phase can retain an additional receptor which has the ability to attract and immobilize the capture reagent. The additional receptor can include a charged substance that is oppositely charged with respect to the capture reagent itself or to a charged substance conjugated to the capture reagent. As yet another alternative, the receptor molecule can be any specific binding member which is immobilized upon (attached to) the solid phase and which has the ability to immobilize the capture reagent through a specific binding reaction. The receptor molecule enables the indirect binding of the capture reagent to a solid phase material before the performance of the assay or during the performance of the assay. The solid phase thus can be a plastic, derivatized plastic, magnetic or non¬ magnetic metal, glass or silicon surface of a test tube, microtiter well, sheet, bead, microparticle, chip, sheep (or other suitable animal's) red blood cells, duracytes and other configurations known to those of ordinary skill in the art. It is contemplated and within the scope of the invention that the solid phase also can comprise any suitable porous material with sufficient porosity to allow access by detection antibodies and a suitable surface affinity to bind antigens. Microporous structures are generally preferred, but materials with gel structure in the hydrated state may be used as well. Such useful solid supports include but are not limited to natural polymeric carbohydrates and their synthetically modified, cross-linked or substituted derivatives, such as agar, agarose, cross-linked alginic acid, substituted and cross-linked guar gums, cellulose esters, especially with nitric acid and carboxylic acids, mixed cellulose esters, and cellulose ethers; natural polymers containing nitrogen; synthetic polymers which may be prepared with suitably porous structures, such as vinyl polymers; porous inorganic materials such as sulfates or carbonates of alkaline earth metals and magnesium, including barium sulfate, calcium sulfate, calcium carbonate, silicates of alkali and alkaline earth metals, aluminum and magnesium; and aluminum or silicon oxides or hydrates, such as clays, alumina, talc, kaolin, zeolite, silica gel, or glass (these materials may be used as filters with the above polymeric materials); and mixtures or copolymers of the above classes, such as graft copolymers obtained by initializing polymerization of synthetic polymers on a pre-existing natural polymer. All of these materials may be used in suitable shapes, such as films, sheets, or plates, or they may be coated onto or bonded or laminated to appropriate inert carriers, such as paper, glass, plastic films, or fabrics. The porous structure of nitrocellulose has excellent absoφtion and adsoφtion qualities for a wide variety of reagents. Nylon also possesses similar characteristics and also is suitable. It is contemplated that such porous solid supports described hereinabove are preferably in the form of sheets of thickness from about 0.01 to 0.5 mm, preferably about 0.1 mm. The pore size may vary within wide limits, and is preferably from about 0.025 to 15 microns, especially from about 0.15 to 15 microns. The surfaces of such supports may be activated by chemical processes which cause covalent linkage of the antigen or antibody to the support. The irreversible binding of the antigen or antibody is obtained, however, in general, by adsoφtion on the porous material by poorly understood hydrophobic forces. Suitable solid supports also are described in U.S. Patent Application Serial No.227,272. The "indicator reagent "comprises a "signal generating compound" (also termed a "label") which is capable of generating and generates a measurable signal detectable by external means conjugated (attached) to a specific binding member for HGBV. "Specific binding member" as used herein means a member of a specific binding pair. That is, two different molecules where one of the molecules through chemical or physical means specifically binds to the second molecule. In addition to being an antibody member of a specific binding pair for HGBV, the indicator reagent also can be a member of any specific binding pair, including either hapten-anti-hapten systems such as biotin or anti-biotin, avidin or biotin, a carbohydrate or a lectin, a complementary nucleotide sequence, an effector or a receptor molecule, an enzyme cofactor and an enzyme, an enzyme inhibitor or an enzyme, and the like. Furthermore, specific binding pairs can include members that are analogs of the original specific binding members, for example, an analyte- analog. An immunoreactive specific binding member can be an antibody or fragment thereof, an antigen or fragment thereof, or an antibody/antigen complex including those formed by recombinant DNA molecules that is capable of binding either to HGBV as in a sandwich assay, to the capture reagent as in a competitive assay, or to the ancillary specific binding member as in an indirect assay. The various "signal generating compounds" (labels) contemplated include chromogens, catalysts such as enzymes, luminescent compounds such as fluorescein and rhodamine, chemiluminescent compounds such as dioxetanes, acridiniums, phenanthridiniums and luminol,- radioactive elements, and direct visual labels. Examples of enzymes include alkaline phosphatase, horseradish peroxidase, beta-galactosidase, and the like. The selection of a particular label is not critical, but it will be capable of producing a signal either by itself or in conjunction with one or more additional substances. A label can be directly detectable, as with, for example, radioisotopes, fluorophores, chemiluminophores, enzymes, colloidal particles, fluorescent microparticles and the like; or a label may be indirectly detectable, as with, for example, specific binding members. It will be understood that direct labels may require additional components such as but not limited to substrates, triggering reagents, light, and the like to enable detection of the label. When indirect labels are used for detection, they are typically used in combination with a conjugate. A "conjugate" is typically a specific binding member which has been attached or coupled to a directly detectable label. Coupling chemistries for synthesizing a conjugate are well known in the art and can include, for example, any chemical means and/or physical means that does not destroy the specific binding property of the specific binding member or the detectable property of the label. The term "hapten", as used herein, refers to a partial antigen or non-protein binding member which is capable of binding to an antibody, but which is not capable of eliciting antibody formation unless coupled to a carrier protein. Examples of haptens include biotin, avidin, adamantine and carbazole. "Analyte," as used herein, is the substance to be detected which may be present in the test sample. The analyte can be any substance for which there exists a naturally occurring specific binding member (such as, an antibody), or for which a specific binding member can be prepared. Thus, an analyte is a substance that can bind to one or more specific binding members in an assay. "Analyte" also includes any antigenic substances such as target nucleotide sequences, haptens, antibodies, and combinations thereof. As a member of a specific binding pair, the analyte can be detected by means of naturally occurring specific binding partners (pairs) such as the use of intrinsic factor protein as a member of a specific binding pair for the determination of Vitamin B 12, the use of folate-binding protein to determine folic acid, or the use of a lectin as a member of a specific binding pair for the determination of a carbohydrate. The analyte can include a protein, a peptide, an amino acid, a nucleotide target of RNA or DNA or of PNA, and the like. Embodiments which utilize ion capture procedures for immobilizing an immobilizable reaction complex with a negatively charged polymer, described in co-pending U. S. Patent Application Serial No. 150,278 coπesponding to EP publication 0326100 and U. S. Patent Application Serial No. 375,029 (EP publication no. 0406473), can be employed according to the present invention to effect a fast solution-phase immunochemical reaction. An immobilizable immune complex is separated from the rest of the reaction mixture by ionic interactions between the negatively charged poly-anion/immune complex and the previously treated, positively charged porous matrix and detected by using various signal generating systems previously described, including those described in chemiluminescent signal measurements as described in co-pending U.S. Patent Application Serial No.921,979 coπesponding to EPO Publication No. 0273,115. Also, the methods of the present invention can be adapted for use in systems which utilize microparticle technology including in automated and semi- automated systems wherein the solid phase comprises a microparticle (magnetic or non-magnetic). Such systems include those described in pending U. S. Patent Applications 425,651 and 425,643, which coπespond to published EPO applications Nos. EP 0425 633 and EP 0424634, respectively. The use of scanning probe microscopy (SPM) for analyte detection also is adaptable. In scanning probe microscopy, in particular in atomic force microscopy, the capture phase is adhered to a solid phase and a scanning probe microscope is utilized to detect antigen/antibody complexes which may be present on the surface of the solid phase. The use of scanning tunneling microscopy eliminates the need for labels which normally must be utilized in many immunoassay systems to detect antigen antibody complexes. Such a system is described in pending U. S. patent application Serial No. 662,147. It is contemplated and within the scope of the present invention that the HGBV group of viruses may be detectable in assays by use of a synthetic, recombinant or native probe that is common to all HGBV viruses. It also is within the scope of the present invention that different synthetic, recombinant or native probes identifying different epitopes from HGBV-A, HGBV-B, HGBV-C, or yet other HGBV viruses, can be used in assay formats. In the later case, these can be coated onto one solid phase, or each separate probe may be coated on separate solid phases, such as microparticles, and then combined to form a mixture of probes which can be later used in assays. Such variations of assay formats are known to those of ordinary skill in the art and are discussed hereinbelow. The reagents and methods of the present invention are made possible by the provision of a family of closely related nucleotide sequences present in the plasma, serum or liver homogenate of an HGBV infected individual, either tamarin or human. This family of nucleotide sequences is not of human or tamarin origin, since it hybridizes to neither human nor tamarin genomic DNA from uninfected individuals, since nucleotides of this family of sequences are present only in liver (or liver homogenates), plasma or serum of individuals infected with HGBV, and since the sequence is not present in GenBank®. In addition, the family of sequences will show no significant identity at the nucleic acid level to sequences contained within the HAV, HBV, HCV, HDV and HEV genome, and low level identity, considered not significant, as translation products. Infectious sera, plasma or liver homogenates from HGBV infected humans contain these polynucleotide sequences, whereas sera, plasma or liver homogenates from non- infected humans do not contain these sequences. Northern blot analysis of infected liver with some of these polynucleotide sequences demonstrate that they are derived from a large RNA transcript similar in size to a viral genome. Sera, plasma or liver homogenates from HGB V-infected humans contain antibodies which bind to this polypeptide, whereas sera, plasma or liver homogenates from non-infected humans do not contain antibodies to this polypeptide; these antibodies are induced in individuals following acute non- A, non-B, non-C, non-D and non- E hepatitis infection. By these criteria, it is believed that the sequence is a viral sequence, wherein the virus causes or is associated with non- A, non-B, non-C, non-D and non-E hepatitis. The availability of this family of nucleic acid sequences permits the construction of DNA probes and polypeptides useful in diagnosing non- A, non-B, non-C, non-D, non-E hepatitis due to HGBV infections, and in screening blood donors, donated blood, blood products and individuals for infection. For example, from the sequence it is possible to synthesize DNA oligomers of about eight to ten nucleotides, or larger, which are useful as hybridization probes or PCR primers to detect the presence of the viral genome in, for example, sera of subjects suspected of harboring the virus, or for screening donated blood for the presence of the virus. The family of nucleic acid sequences also allows the design and production of HGBV specific polypeptides which are useful as diagnostic reagents for the presence of antibodies raised during infection with HGBV. Antibodies to purified polypeptides derived from the nucleic acid sequences may also be used to detect viral antigens in infected individuals and in blood. These nucleic acid sequences also enable the design and production of polypeptides which may be used as vaccines against HGBV, and also for the production of antibodies, which then may be used for protection of the disease, and/or for therapy of HGBV infected individuals. Using determined portions of the isolated HGBV nucleic acid sequences as a basis, oligomers of approximately eight nucleotides or more can be prepared, either by excision or synthetically, which hybridize with the HGBV genome and are useful in identification of the viral agent(s), further characterization of the viral genome, as well as in detection of the virus(es) in diseased individuals. The natural or derived probes for HGBV polynucleotides are a length which allows the detection of unique viral sequences by hybridization. While six to eight nucleotides may be a workable length, sequences of ten to twelve nucleotides are prefeπed, and those of about 20 nucleotides may be most prefeπed. These sequences preferably will derive from regions which lack heterogeneity. These probes can be prepared using routine, standard methods including automated oligonucleotide synthetic methods. A complement of any unique portion of the HGBV genome will be satisfactory. Complete complementarity is desirable for use as probes, although it may be unnecessary as the length of the fragment is increased. When used as diagnostic reagents, the test sample to be analyzed, such as blood or serum, may be treated such as to extract the nucleic acids contained therein. The resulting nucleic acid from the sample may be subjected to gel electrophoresis or other size separation techniques; or, the nucleic acid sample may be dot-blotted without size separation. The probes then are labeled. Suitable labels and methods for attaching labels to probes are known in the art, and include but are not limited to radioactive labels incoφorated by nick translation or kinasing, biotin, fluorescent and chemiluminescent probes. Examples of many of these labels are disclosed herein. The nucleic acids extracted from the sample then are treated with the labeled probe under hybridization conditions of suitable stringencies. The probes can be made completely complementary to the HGBV genome. Therefore, usually high stringency conditions are desirable in order to prevent false positives. However, conditions of high stringency should be used only if the probes are complementary to regions of the HGBV genome which lack heterogeneity. The stringency of hybridization is determined by a number of factors during the washing procedure, including temperature, ionic strength, length of time and concentration of formamide. See, for example, J. Sambrook (supra). Hybridization can be carried out by a number of various techniques. Amplification can be performed, for example, by Ligase Chain Reaction (LCR), Polymerase Chain Reaction (PCR), Q-beta replicase, NASBA, etc. It is contemplated that the HGBV genome sequences may be present in serum of infected individuals at relatively low levels, for example, approximately 10-*-- 10- sequences per ml. This level may require that amplification techniques such as the LCR or the PCR be used in hybridization assays. Such techniques are known in the art. For example, the "Bio-Bridge" system uses terminal deoxynucleotide transferase to add unmodified 3'-poly-dT-tails to a nucleic acid probe (Enzo Biochem. Coφ.). The poly dt-tailed probe is hybridized to the target nucleotide sequence, and then to a biotin-modified poly-A. Also, in EP 124221 there is described a DNA hybridization assay wherein the analyte is annealed to a single-stranded DNA probe that is complementary to an enzyme-labeled oligonucleotide, and the resulting tailed duplex is hybridized to an enzyme-labeled oligonucleotide. EP 204510 describes a DNA hybridization assay in which analyte DNA is contacted with a probe that has a tail, such as a poly-dT-tail, an amplifier strand that has a sequence that hybridizes to the tail ofthe probe, such as a poly-A sequence, and which is capable of binding a plurality of labeled strands. The technique first may involve amplification ofthe target HGBV sequences in sera to approximately 10^ sequences/ml. This may be accomplished by following the methods described by Saiki et al., Nature 324: 163 (1986). The amplified sequence(s) then may be detected using a hybridization assay such as those known in the art. The probes can be packaged in diagnostic kits which include the probe nucleic acid sequence which sequence may be labeled; alternatively, the probe may be unlabeled and the ingredients for labeling could be included with the kit. The kit also may contain other suitably packaged reagents and materials needed or desirable for the particular hybridization protocol, for example, standards as well as instructions for performing the assay. Other known amplification methods which can be utilized herein include but are not limited to the so-called "NASBA" or "3SR" technique taught in PNAS USA 87:1874-1878 (1990) and also discussed in Nature: 350 (No. 6313):91-92 (1991) and Q-beta replicase. Fluorescence in situ hybridization ("FISH") also can be performed utilizing the reagents described herein. In situ hybridization involves taking moφhologically intact tissues, cells or chromosomes through the nucleic acid hybridization process to demonstrate the presence of a particular piece of genetic information and its specific location within individual cells. Since it does not require homogenization of cells and extraction ofthe target sequence, it provides precise localization and distribution of a sequence in cell populations. In situ hybridization can identify the sequence of interest concentrated in the cells containing it. It also can identify the type and fraction of the cells in a heterogeneous cell population containing the sequence of interest. DNA and RNA can be detected with the same assay reagents. PNAs or moφholino compounds can be utilized in FISH methods to detect targets without the need for amplification. If increased signal is desired, multiple fluorophores can be used to increase signal and thus, sensitivity of the method. Various methods of FISH are known, including a one-step method using multiple oiigonucleotides or the conventional multi-step method. It is within the scope of the present invention that these types of methods can be automated by various means including flow cytometry and image analysis. Assays as described herein may utilize one viral antigen derived from any clone-containing HGBV nucleic acid sequence, or from the composite nucleic acid sequences derived from the HGBV nucleic acid sequences in these clones, or from the HGBV genome from which the nucleic acid sequences in these clones are derived. Or, the assay may use a combination of viral antigens derived from these sources. It also may use, for example, a monoclonal antibody directed against the same viral antigen, or polyclonal antibodies directed against different viral antigens. Assays can include but are not limited to those based on competition, direct reaction or sandwich-type assays. Assays may use solid phases or may be performed by immunoprecipitation or any other methods which do not utilize solid phases. Examples of assays which utilize labels as the signal generating compound and those labels are described herein. Signals also may be amplified by using biotin and avidin, enzyme labels or biotin anti-biotin systems, such as that described in pending U.S. patent application Serial Nos. 608,849; 070,647; 418,981; and 687,785. The HGBV nucleic acid sequences may be used to gain further information on the sequence of the HGBV genome and for identification and isolation of the HGBV agent. Thus, it is contemplated that this knowledge will aid in the characterization of HGBV including the nature of the HGBV genome, the structure of the viral particle and the nature of the antigens of which it is composed. This information, in turn, can lead to additional polynucleotide probes, polypeptides derived from the HGBV genome, and antibodies directed against HGBV epitopes which would be useful for the diagnosis and/or treatment of HGBV caused non- A, non-B, non-C, non-D and non-E hepatitis. Synthetic oiigonucleotides may be prepared using an automated oligonucleotide synthesizer such as that described by Warner, DNA 3:401 (1984). If desired, the synthetic strands may be labeled with 32p by treatment with polynucleotide kinase in the presence of ----'P-ATP, using standard conditions for the reaction. DNA sequences including those isolated from genomic or cDNA libraries, may be modified by known methods which include site directed mutagenesis as described by Zoller, Nucleic Acids Res. 10:6487 (1982). Briefly, the DNA to be modified is packaged into phage as a single stranded sequence, and converted to a double stranded DNA with DNA polymerase using, as a primer, a synthetic oligonucleotide complementary to the portion of the DNA to be modified, and having the desired modification included in its own sequence. Culture of the transformed bacteria, which contain replications of each strand of the phage, are plated in agar to obtain plaques. Theoretically, 50% of the new plaques contain phage having the mutated sequence, and the remaining 50% have the original sequence. Replicates of the plaques are hybridized to labeled synthetic probe at temperatures and conditions suitable for hybridization with the conect strand, but not with the unmodified sequence. The sequences which have been identified by hybridization are recovered and cloned. Polymerase chain reaction (PCR) and ligase chain reaction (LCR) are techniques for amplifying any desired nucleic acid sequence (target) contained in a nucleic acid or mixture thereof. In PCR, a pair of primers are employed in excess to hybridize at the outside ends of complementary strands ofthe target nucleic acid. The primers are each extended by a polymerase using the target nucleic acid as a template. The extension products become target sequences themselves, following dissociation from the original target strand. New primers are then hybridized and extended by a polymerase, and the cycle is repeated to geometrically increase the number of target sequence molecules. PCR is disclosed in U.S. patents 4,683,195 and 4,683,202. LCR is an altemate mechanism for target amplification. In LCR, two sense (first and second) probes and two antisense (third and fourth) probes are employed in excess over the target. The first probe hybridizes to a first segment of the target strand and the second probe hybridizes to a second segment of the target strand, the first and second segments being positioned so that the primary probes can be ligated into a fused product. Further, a third (secondary) probe can hybridize to a portion of the first probe and a fourth (secondary) probe can hybridize to a portion of the second probe in a similar ligatable fashion. If the target is initially double stranded, the secondary probes will also hybridize to the target complement in the first instance. Once the fused strand of sense and antisense probes are separated from the target strand, it will hybridize with the third and fourth probes which can be ligated to form a complementary, secondary fused product. The fused products are functionally equivalent to either the target or its complement. By repeated cycles of hybridization and ligation, amplification of the target sequence is achieved. This technique is described in EP-A-320,308, hereby incoφorated by reference. Other aspects of LCR technique are disclosed in EP-A-439, 182, which is incoφorated herein by reference. Oiigonucleotides are provided which are useful for the detection of HGB V- C. These oiigonucleotides detect isolates of HGBV-C but do not detect isolates of HGBV-A or HGBV-B. These primers are designated as SEQUENCE ID NO. 51, SEQUENCE ID NOS 53-57 and SEQUENCE ID NO 87. Other primers, designated as SEQUENCE ID NO. 27 through SEQUENCE ID NO 35, are useful for classifying genotypes of HGBV-C. As a result of studying nucleotide sequences of HGBV-C isolates, it has been found that these isolates can be divided into at least four genotypes based upon the nucleotide sequences located near the 5' end of the genome. The primers are useful in amplification procedures described previously herein such as PCR. Other primers which can differentiate between the genotypes can be used in PCR, while other primers are useful in GAP LCR. These are described in the following examples. These primers thus provide a method of detecting HGBV-C genotypes. As detailed hereinbelow, 5' end cDNA was obtained by reverse transcription with random hexamer primers, and the cDNA was amplified using other oligonucleotide primer(s) as a sense and antisense primer(s) in a PCR reaction. In some cases, this PCR reaction was followed by amplification using other primers as sense and antisense primers in a second stage PCR. As shown hereinbelow, we classified 46 HGBV-C isolates obtained from 39 individuals into four genotypes. These genotypes exhibited a maximum sequence divergence of 17.4%. The HGBV-C oiigonucleotides described herein are useful in detecting HGBV-C nucleic acids in a test sample. The genotyping of HGBV-C isolates also will aid in prognostic studies as well as in prevention and treatment of the disease caused by HGBV-C in humans. The present invention will now be described by way of examples, which are meant to illustrate, but not to limit, the spirit and scope of the invention. EXAMPLES Example 1. Detection of HGBV-C RNA As described by us previously in U.S. Serial No. 08/473,475 (previously incoφorated herein by reference), the generation of HGB V-C-specific ELISAs has allowed the identification of immunopositive sera in each of several categories of human populations, including intravenous drug users, residents of West Africa, volunteer blood donors and individuals with or at risk for non- A-E hepatitis. Sera from these seropositive individuals were tested for HGBV-C viremia by the RT- PCR assays described briefly as follows, and several serum samples were found to be positive for HGBV-C viral RNA. RT-PCR was performed using degenerative NS3 oligonucleotide primers (SEQUENCE ID NOS. 88 and 89) in a single round of amplification employing a thermocycling protocol designed to amplify specific products with oligonucleotide primers that may contain base pair mismatches with the template to be amplified (Roux, Bio/Techniques 16:812-814 [1994]). Specifically, reactions were thermocycled 43 times (94°C, 20 sec; 55°C decreasing 0.3°C/cycle, 30 sec; 72°C, 1 min) followed by 10 cycles (94°C, 20 sec; 40°C, 30 sec; 72°C, 1 min) with a final extension at 72°C for 10 minutes. PCR products were separated by agarose gel electrophoresis, visualized by UV iπadiation after direct staining of the nucleic acid with ethidium bromide, then hybridized to a radiolabeled probe for GB-C (SEQUENCE ID NO 26, from position 4245 to 4432) after Southern transfer to Hybond-N+ nylon filter (available from Amersham Life Sciences, Arlington Heights, IL). Testing by RT- PCR of additional seropositive individuals from each of the populations listed above demonstrated a correlation between antibody presence and detection of viral RNA. At that time, the PCR amplified products from 26 of these individuals were cloned into the vector pT7Blue and sequenced, following methods as described in the art. Alignment of these sequences, each derived from the highly conserved portion of the HGBV-C NS3 helicase gene (i.e. nucleotides 4272-4407 of HGB V- C, SEQUENCE ID NO. 26; 135 nucleotides in length), and subsequent phylogenetic analysis did not demonstrate a significant grouping of the sequences into subtypes (data not shown). Phylogenetic analysis has been applied to the hepatitis C viruses and has demonstrated that the variability among HCV isolates delineated six equally divergent main subtypes of sequences. See, for example, Simmonds, P. et al., Gen. Virol. 74:2391-2399 (1993) and Simmonds, P. et al., J. Gen. Virol. 75: 1053-1061] (1994). This analysis resulted in the establishment of a system of nomenclature for the hepatitis C viruses, as reported by Simmonds, P. et al. Hepatology. 19:1321-1324 (1994), where the isolates were classified into genotypes based upon the evolutionary distance between groups of sequences. In the case of HCV, infection with specific viral genotypes has been conelated with severity of disease and responsiveness to treatment with antiviral drugs such as interferon-2alpha. See, for example, Dawson, G., et al. "Recent developments in the molecular biology of the hepatitis viruses" in Cunent Hepatology. G. Gitnick (Ed)., St. Louis, Mo., Mosby (1995). To examine the possible conelation between severity of disease or treatment outcomes and infection with specific HGBV-C subtypes, it was first necessary to determine whether such subtypes existed. However, the data obtained at that time from analysis of the NS3 sequences had not demonstrated the presence of genotypes (as described herein); this was due to the limited amount of sequence data obtained from each isolate (i.e., only 135 nucleotides). Thus, in order to obtain sufficient data to accurately determine the phylogenetic relationships between HGBV-C isolates, experiments were conducted to amplify extended regions from the 5 '-end ofthe HGBV-C genome of various HGBV-C isolates. Sera from individuals previously shown to be RT-PCR positive for HGBV-C as described hereinabove were used as the source of HGBV-C viral RNA. Specifically, two oligonucleotide primers, SEQ ID NO. 51 (ntrC-Sl) and SEQUENCE ID NO 52 (G131-E1 wb2), located near the 5'-terminus of the HGBV-C genome and near the N-terminus of the putative El gene, respectively, were utilized in the thermocycling protocol known in the art and previously described hereinabove, on serum-derived cDNA products generated as known in the art. In some experiments, other oligonucleotide primers (i.e., ntrC-S2 [SEQUENCE ID NO 53] combined with ntrC-4R [SEQUENCE ID NO 57] and ntrC-Sl [SEQUENCE ID NO 51] combined with ntrC-4R [SEQUENCE ID NO 57] derived from the 5' end of the HGBV-C genome were used in PCR experiments detailed herein to amplify smaller regions ofthe HGBV-C genome. Using these primers, 46 PCR products (SEQUENCE ID NOS 2 through 6, SEQUENCE ID NO 9 through 11, SEQUENCE ID NO 13 through 24 and SEQUENCE ID NO 60 through 86) (TABLE 1) were obtained from 39 individuals previously shown to be HGBV-C RNA positive: Four of these isolates were from individuals classified as indeterminate for the presence of antibodies to hepatitis C virus proteins, 21 of these isolates were from individuals from a region of West Africa where infection with other hepatitis viruses is endemic (this includes the coπesponding sequence from the HGBV-C genome, SEQUENCE ID NO 26, in TABLE 1, hereinbelow), four of these isolates were from non A-E hepatitis patients, five of these isolates were from patients diagnosed with aplastic anemia, six of these isolates were from multiply transfused individuals, three of these isolates were from normal blood donors from the U.S., one of these isolates was from an intravenous drug user (IVDU) and two of these isolates were from two individuals from southeast Asia. These products were cloned into the bacterial plasmid pTVBlue and sequenced following procedures known in the art. The HGBN-C isolates obtained are listed in TABLE 1, including a description of the individual from which the isolates were obtained. In some cases multiple isolates obtained from a single source were sequence analyzed; specifically: SEQUENCE ID NOS 16, 20 and 84 were obtained from an individual diagnosed with aplastic anemia; SEQUENCE ID NOS 21 and 22 were obtained from a multiply transfused individual; SEQUENCE ID NOS 67 and 68, 69 and 70, 75 and 76 were obtained from three individuals from West Africa, respectively. TABLE 1. HGBN-C Isolates SEQ Genotype Description SEQ Genotype Description ID # ID # 2 West Africa 64 1 West Africa 3 West Africa 65 1 West Africa 4 West Africa 66 1 West Africa 5 West Africa 67 1 West Africa 6 West Africa 68 1 West Africa 9 2 HCV Indeterminate 69 1 West Africa 10 2 Aplastic Anemia 70 1 West Africa 11 2 Normal Donor 71 1 West Africa 13 2 Multiply Transfused 72 1 West Africa 14 2 HCV Indeterminate 73 1 West Africa 15 2 HCV Indeterminate 74 1 West Africa 16 3 Aplastic Anemia 75 1 West Africa 17 3 Non-A-thru-Non-Eφ 76 1 West Africa 18 3 Non-A-thru-Non-EI 77 1 West Africa 19 3 Non-A-thru-Non-Eφ 78 4 Southeast Asia 20 3 HCV Indeterminate 79 2 Non-A-thru-Non-Eφ 21 3 Multiply Transfused 80 1 West Africa 22 3 Multiply Transfused 81 2 Aplastic Anemia 24 3 Multiply Transfused 82 3 Normal Donor 60 2 Aplastic Anemia 83 1 West Africa 61 2 Multiply Transfused 84 2 Aplastic Anemia 62 2 Multiply Transfused 85 2 Intravenous Drug User 63 4 Southeast Asia 86 2 Non-A-thru-Non-E$ Alignment of these sequences was made using the program PILEUP of the Wisconsin Sequence Analysis Package (Version 8) and is shown in FIGURE 1 A- FIGURE IF. Referring to FIGURE 1 A-1F, the consensus nucleotide at each position in the alignment was determined by the base that occurred most frequently at that position. The consensus line does not necessarily represent the consensus sequence of the "prototype" GB virus C isolate. The dashes (-) in FIGURE 1 A- 1F represent bases identical to that shown on the consensus line which is indicated as "cons" in this FIGURE. Base deletions are indicated by periods (.) in this FIGURE. For each isolate, bases are shown only at those positions in the alignment that differ from the consensus. The sequence of the PCR primers used to amplify the isolates are not shown the alignment of this FIGURE. The phylogenetic relationship between these GB virus C sequences (isolates) was examined by calculating the evolutionary distance between the aligned nucleotide sequences using the DNADIST program of the PHYLIP package (version 3.5c, 1993) kindly provided by J. Felsenstein (see Felsenstein, J. Cladistics 5:164-166 [1989]). These computed distances were used for the construction of phylogenetic trees using the program FTTCH. A tree providing a graphical representation of the distance between sequences was plotted using the program DRAWGRAM and is shown in FIGURE 2. The "root" of the tree is arbitrarily assigned to the midpoint of the longest branch of the tree for clarity of display, however, the tree shown is unrooted; i.e. the ancestor common to all sequences in the tree has not been established. The results of this analysis indicated that there are at lease four distinct genotypes of GB virus C isolates. The relatedness of these individual sequences is presented graphically in FIGURE 2. Two of these groups, i.e., genotypes 1 and 4, are significantly distant from each other and the other two groups, i.e., genotypes 2 and 3. Up to this time, Genotype 1 isolates of HGBV-C have been found exclusively among individuals from West Africa and have included the original HGBV-C isolate. Sequences belonging to Genotypes 2 and 3 isolates have not demonstrated, to date, a specific geographic distribution. Complete clinical information regarding disease status or treatment regimes has not been available for all of these individuals from which sequences were obtained. Thus, it has not been cuπently possible to coπelate disease severity with infecting HGBV- C genotype. Collection of clinical information is ongoing. Of interest, however, is the observations that some individuals may be infected with more than one genotype of HGBV-C, since one patient diagnosed with aplastic anemia has been found to be infected with HGBV-C genotypes 2 and 3 (SEQUENCE ID NOS 12, 16 AND 60). A large amount of sequence variation was found to be present in the various HGBV-C isolates from both the NS3 and 5 '-terminal region. Although highly sensitive, PCR based assays for viral nucleic acids are dependent on the sequence match between oligonucleotide primers and the viral template. Therefore, because the PCR primers which were utilized to amplify sequences from the NS3 region and the 5 '-terminal portion (GBV-C [SEQUENCE ID NO 51] - Elwb2 [SEQUENCE ID NO 52]) of the genome were located in regions that were not well conserved in all isolates, not all HGBV-C viremic samples tested may have been detected by the RT-PCR assays employed here. It was hypothesized that utilization of PCR primers from a highly conserved region of the HGBV-C genome, as have been found in the HCV 5' untranslated region [Cha, et al. J. Clin. Microbiol. 29:2528-2534 (1991)], should allow more accurate detection of HGBV-C viremic samples. Thus, examination of the aligned sequences presented in FIGURE 1 generated from all 46 isolates demonstrated the presence of several regions of highly conserved nucleotide sequences among all the isolates. Primers prepared from these highly conserved regions of HGBV-C, ntrC-S2 (SEQUENCE ID NO 53), ntrC-Al (SEQUENCE ID NO 54), ntrC-A2 (SEQUENCE ID NO 55), ntrC-3F (SEQUENCE ID NO 56), and ntrC-4R (SEQUENCE ID NO 57) were developed to examine the sensitivity and specificity of these universal HGBV-C primers versus the degenerate primers from the HGBV-C helicase gene. The primers ntrC-S 1/ntrC-al (SEQUENCE ID NO 51/SEQUENCE ID NO 54) and ntrC-S2/ntrC-a2 (SEQUENCE ID NO 53/SEQUENCE ID NO 55) were used in independent PCRs or in combination in a nested PCR experiment. The primers ntrC-3F/ntrC-4R (SEQUENCE ID NO 56/SEQUENCE ID NO 57) were used in combination in separate PCRs. These PCR amplification procedures, well-known to those of ordinary skill in the art, were as follows. Briefly, the first round amplification was performed on serum cDNA products generated as described earlier, using 2 mM MgCl2 and 1 μM primers (both sense and antisense), as follows. Reactions were subjected to 35-40 cycles of denaturation-annealing-extension (94°C, 20 sec; 55°C, 30 sec; 72°C, 45 sec) followed by a 10 min extension at 72°C. Completed reactions were held at 4°C. A second round of amplification performed as either a fully nested or a hemi-nested reaction, if necessary, was performed utilizing 2 mM MgCl2, 1 μM sense and antisense primers and 4% of the first PCR products as template. The second round of amplification employed a thermocycling protocol identical that utilized in the first round of PCR. PCR products were separated by agarose gel electrophoresis and visualized by UV irradiation after direct staining of the nucleic acid with ethidium bromide. Unless a nested PCR reaction was performed, the products of a single round of PCR amplification were transfened to Hybond-N+ nylon filter and then hybridized to a radiolabeled probe for HGBV-C. Results obtained from these experiments confirmed the presence of HGBV-C RNA in 38 out of 39 individuals whose sera had previously tested positive for HGBV-C RNA by using the helicase region primers as stated hereinabove. We hypothesized that the single individual whose serum tested negative with the HGBV-C universal 5'- end primers may have originally been detected positive with the NS3 primers due to contamination of the serum sample with amplicon derived from another sample or with HGBV-C RNA-positive sera from another individual. However, primer pairs derived from highly conserved regions of these HGBV-C isolates have been shown to be useful as a method to test for or and/or confirm HGBV-C viremia. In order to determine which oligonucleotide primers derived from highly conserved regions within the 5'-end of the HGBV-C genome would be the most useful for detecting HGBV-C RNA, RT-PCR experiments were conducted in which various sense primers (ntrC-Sl [SEQUENCE ID NO 51], ntrC-3F [SEQUENCE ID NO 56), ntrC-Al [SEQUENCE ID NO 54], ntrC-A2 [SEQUENCE ID NO 55], ntrC-4R [SEQUENCE ID NO 57], ntrC-5R [SEQUENCE ID NO 87]). Human sera obtained from 12 commercial blood - donors that had tested positive for GBV-C RNA with PCR primers derived from the NS3 helicase region (using SEQUENCE ID NOS 88 and 89 and amplification procedures disclosed hereinabove, data not shown) were retested by using eleven difference combinations of HGBV-C 5'-end sense/antisense primers. Briefly, amplification was performed on serum cDNA products generated as described hereinabove, using 2 mM MgCl2 and 1 μM sense and antisense primers. PCR reactions utilized 40 cycles of denaturation-annealing-extension (94°C, 20 sec.; 55°C, 30 sec; 72°C, 45 sec.) followed by a ten minute extension at 72-C. Completed reactions were held at 4°C. Amplification reactions utilized only 40% of the amount of cDNA used in the initial testing of these samples with NS3 degenerate primers. Thus, in order to compare the usefulness of the 5'-end derived primers with the NS3 degenerate primers, the samples were retested in parallel for HGBV-C RNA using primers derived from the NS3 helicase gene (SEQUENCE ID NOS 88 and 89) following the methods as described hereinbelow. Amplification with NS3 degenerate HGBV-C primers was conducted using a thermocycling protocol designed to amplify DNA sequences that may contain base pair mismatches between the template and the primer(s) as described by Roux, BioTechniques 16:812-814 (1994)]. Specifically, reactions were thermocycled 43 times (94°C, 20 sec; 55°C decreasing 0.3°C/cycle, 30 sec; 72°C, 1 min) followed by 10 cycles (94°C, 20 sec; 40°C, 30 sec; 72°C, 1 min) with a final extension at 72°C for 10 minutes. PCR products were separated by agarose gel electrophoresis and visualized by UV inadiation after direct staining of the nucleic acid with ethidium bromide. The nucleic acids then were transfeπed by Hybond-N+ nylon filters (available from Amersham Life Sciences, Arlington Heights, EL) and then hybridized to the appropriate radiolabeled probe for HGB V- C (SEQUENCE ID NO 26, positions 13 to 631 ). Results of these experiments are summarized in TABLES 2 A and 2B. As the results demonstrate, the primer pair ntrC-S l/ntrC-2A (SEQUENCE ID NO 51/SEQUENCE ID NO 55) detected HGBV-C RNA in all 11/12 specimens as determined by ethidium bromide staining (TABLE 2A) and 12/12 specimens as determined by southem blot analysis (TABLE 2B). In contrast, ntr-C-3F/ntrC-4R (SEQUENCE ID NO 56/SEQUENCE ID NO 57) detected only 4/12 specimens as determined by ethidium bromide staining and 7/12 specimens as determined by southern hybridization. In addition, the NS3-derived HGBV-C degenerate primers (SEQUENCE ID NO 88 and SEQUENCE ID NO 89) detected RNA in 7/12 specimens as determined by ethidium bromide staining and 8/12 specimens by southern analysis. These data indicate that some HGBV-C 5'-end primers pairs may be more sensitive than others for detecting HGBV-C viremia, even though all 5'-end primers are derived from regions exhibiting a high degree of nucleotide sequence conservation among all HGBV-C isolates. In addition, under the conditions utilized, the HGBV-C NS3 helicase derived PCR primers may not be as sensitive as the primers derived from the 5' end of the genome for the detection of HGBV-C nucleic acids. TABLES 2A and 2B indicate primer pairs wherein Sl conesponds to SEQUENCE ID NO 51, S2 coπesponds to SEQUENCE ID NO 53, 5R conesponds to SEQUENCE ID NO 87, 3F coπesponds to SEQUENCE ID NO 56, Al conesponds to SEQUENCE ID NO 54, A2 coπesponds to SEQUENCE ID NO 55, 4R coπesponds to SEQUENCE ID NO 57 and NS3 coπesponds to SEQUENCE ID NO 88 and 89. TABLE 2A. Summarv of GBV-C Primer Pair Testing: Ethidium Bromide Results $ nd means not determined. TABLE 2B. Summarv of GBV-C Primer Pair Testing: Southern Blotting Results t nd means not determined. Example 2. Differential detection of HGBV-C Genotypes 1. 2 and 3 Oligonucleotide primers are used in amplification reactions to differentially detect HGBV-C genotype 1 or 2 or 3, as follows. These primers, HGBV-C-la-sl (SEQUENCE ID NO 28), HGBV-C-la-s2 (SEQUENCE ID NO 32), HGBV-C- la-al (SEQUENCE ID NO 27), HGBV-C-la-a2 (SEQUENCE ID NO 31), HGBV-C-lbc-sl (SEQUENCE ID NO 29), HGBV-C-lbc-s2 (SEQUENCE ID NO 33), HGBV-C-lbc-al (SEQUENCE ID NO 30), HGBV-C-lbc-a2 (SEQUENCE ID NO 34), can be used in amplification reactions where each primer is paired with one of the sense or antisense primers listed above in Example 1 and following the PCR protocols presented in Example 1. Primers listed above with a "la" designation are selective for HGBV-C genotype 1; primers with a "lb" designation are selective for HGBV-C genotype 2; primers with a "lc" designation are selective for HGBV-C genotype 3. The genotype of the particular HGBV-C isolate is determined by the presence or absence of a PCR amplification product of the predicted size as visualized by agarose gel electrophoresis and ethidium bromide staining. In addition, genotypes can be identified by determining the nucleic acid sequence of the PCR amplification product produced using one of the HGBV-C primer pairs. The determined sequence is then compared with the sequence from the homologous region of other HGBV-C isolates. This is accomplished by sequence alignment and subsequent phylogenetic analysis using methods known in the art. Example 3: GAP LCR Detection of HGBV-C Genotypes We have observed that some regions within the HGBV-C sequences are significantly different among the isolates. These sequences were utilized for the construction of oligonucleotide primers that can be used in a GAP-LCR assay to distinguish between members of HGBV-C genotypes 1, 2 or 3. Briefly, double-gap LCR is performed as follows and as detailed in U.S. Patent No. 5, 427,930, previously incoφorated herein by reference. Double gaps are represented herein as "DG p, q" where in "p" is the number of bases in the gap of one strand, and "q" is the number of bases in the gap of the other strand. Thus, a prefened double gap embodiment has two bases missing from each of the two probes whose 5' end participates in the ligation, and is designated as DG2,2. In this prefeπed embodiment, the 3' ends of the other two probes do not overlap; rather, they terminate at the same point on the target strand (and its complement). The procedure is outlined as follows. Double gap LCR is performed for 30-50 cycles consisting of a 65 second incubation at 85°C and a 65 second incubation at 50°C. The oiigonucleotides used are presented hereinbelow as SEQUENCE ID NOS. 35 through 50, and are specific for the 5' end of HGBV-C. Reactions are run in a buffer containing 50 mM EPPS pH 7.8, 100 mM KCI, 10 mM MgCl2, 1 mM DTT, 10 mM NH4CI, 100 μm NAD, 10 μg ml BSA, 5 x 10 1 1 each oligonucleotide listed hereinabove, 1 μm 2' -deoxyguanosine 5'triphosphate, 0.5 units Thermus DNA polymerase (Molecular Biology Resources, Inc., "MBR"), and 3400 units Thermus thermohilus DNA ligase. Reaction volume is 50 μl and each reaction is overlaid with 25 μl of mineral oil prior to cycling. Following amplification, reactions are diluted 1:1 with IM X ® diluent buffer (available from Abbott Laboratories, Abbott Park, IL), or other suitable buffer. The LCR amplification products are detected via a sandwich immunoassay performed using the Abbott IMχ® automated immunoassay system. Example 4. Intemal ribosome entry site in 5' NTR of GBV-B Several positive strand RNA viruses, such as picornaviruses and pestiviruses, possess large 5' nontranslated regions (NTRs). These large NTRs control the initiation of cap-independent translation by functioning as intemal ribosome entry sites (IRESs) (Pelletier and Sonenberg, Nature (London) 334:320- 325). The IRES is thought to form a specific RNA structure which allows ribosomes to enter and begin translation of an RNA without using the cellular machinery required for cap-dependent translation initiation. The large 5' NTR of HCV has been shown to possess an IRES (Wang et al. J. Virol. 67:3338-3344, 1993). Due to the high level of sequence conservation between the 5' NTRs of GBV-B and HCV, it was reasoned that GBV-B may also contain an IRES. To test for IRES function in GBV-B, the 5' NTR of this virus was used to replace the 5' NTR of hepatitis A virus (HAV) in the pLUC-HAV-CAT plasmid described by Whetter et al. ( J. Virol. 68:5253-5263, 1994). The 5' NTR of GBV- B was amplified from a plasmid clone using SEQUENCE ID NO. 58 (UTR-B.l) and SEQUENCE ID. NO. 59 (NTR-B-al) as primers Briefly, a 50 μl PCR was set up using a Perkin-Elmer PCR kit as described by the manufacturer with 1 μM primers, 2 mM MgCl2 and approximately 10 ng of plasmid. This reaction was amplified for 20 cycles (94°C, 20 sec; 55°C, 30 sec; 72°C, 30 sec) followed by a final extension at 72°C for 10 min. The completed reaction then was held at 4°C. This product was extracted with phenol hloroform and precipitated as described in the art. The 3' terminal adenosine residues added by the AmpliTaq® polymerase were removed from this product by incubation with T4 DNA polymerase and deoxynucleotide triphosphates as described (Sambrook et al, Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, 1989). After heat inactivation, the product was digested with Xba I and gel purified as described in the art. The purified product was ligated to pHAV-CATl (Whetter et al. J. Virol. 68:5253- 5263, 1994) that had been cut with HindlH, end-filled with Klenow polymerase and deoxynucleotide triphosphates, heat-inactivated, digested with Xba I, treated with bacterial alkaline phosphatase, extracted with pheno chloroform, and precipitated as described in the art. The constructed plasmid, pGBB-CATl, was digested with Sac I, blunt-ended with T4 DNA polymerase and deoxynucleotide triphosphates, heat-inactivated, and digested with Not I as described in the art. The 1.3 kbp product from these reactions was gel purified and cloned into pLUC- HAV-CAT (Whetter et al. J. Virol. 68:5253-5263, 1994) that had been digested with HindlH, end-filled with Klenow polymerase and deoxynucleotide triphosphates, heat-inactivated, digested with Not I, treated with bacterial alkaline phosphatase, extracted with phenolxhloroform, and precipitated as described in the art. The resultant plasmid, pLUC-GBB-CAT was used in in vitro transcription-translation experiments to test for an IRES function. An in vitro transcription-translation assay was performed using the TNT™ T7 coupled reticulocyte lysate system from Promega (Madison, WI) as described by the manufacturer. The plasmids tested were pLUC-GBB-CAT (described above), pLUC-HAV-CAT (positive control from Whetter et al. J. Virol. 68:5253- 5263, 1994), and pLUC-Δ355-532 (negative control from Whetter et al. J. Virol. 68:5253-5263, 1994). The products (labeled with 35 S -methionine) were run on a 10% Laemmli gel as described in the art. The gel was fixed in 10% methanol, 20% acetic acid for 10 minutes, dried down and exposed to a Phospholmager® screen (Molecular Dynamics, Sunnyvale, CA). The products were visualized with the Phospholmager® (data not shown). All three reactions contained a band consistent with the size expected for luciferase (the LUC gene in the plasmid). LUC expression, which is a measure of the level of translation that initiates from the 5' end of the mRNA, appeared to be equivalent in the three reactions. Thus, equivalent amounts of RNA templates were present in a translatable form in these three reactions. The pLUC-HAV-CAT and the pLUC-GBB-CAT reactions also had a band consistent with the size expected for chloramphenicol acetyltransferase (the CAT gene in the plasmid). This band is not seen in the pLUC-Δ355-532 negative control. CAT expression measures the level of intemal translation initiation. Approximately equivalent levels of- CAT expression are found in pLUC-HAV-CAT and pLUC-GBB-CAT- programmed lysates. Because translation of the CAT gene requires the existence of an IRES in this plasmid construct, the 5' NTR of GBV-B must be providing this function. Therefore, similar to HCV, GBV-B's 5'NTR contains an IRES. Further studies of these plasmids, both in vitro and in vivo are ongoing to better characterize the IRES in GBV-B. The present invention is intended to be limited only by the appended claims. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Muerhoff, A. S. Simons, J. N. Leary, T. P. Birkenmeyer, L. Erker, J. C. Chalmers, M. Dawson, G. J. Desai, S. M Mushahwar, I. K. (ii) TITLE OF INVENTION: Detection of Hepatitis GB Virus Genotypes (iii) NUMBER OF SEQUENCES: 89 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Abbott Laboratories (B) STREET: 100 Abbott Park Rd (C) CITY: Abbott Park (D) STATE: IL (E) COUNTRY: USA (F) ZIP: 60064 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.30 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Porembski, Priscilla E. (B) REGISTRATION NUMBER: 33,207 (C) REFERENCE/DOCKET NUMBER: 5793.US.01 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 708-937-0378 (B) TELEFAX: 708-938-2623 (2) INFORMATION FOR SEQ ID Nθ:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 589 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID Nθ:l: ACCGACGCCT ATCAAAATAG ACGTGATGGC CCCGCGCCGA ACTGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCATCCT GGTAGCCACT 120 ATAGGTGGGT CTTAAAGGGA GGCTACGGTC CCTCTCGCGC TTACGGAGAG AAAGCGCACG 180 GTTCACAGGT GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC 240 CGAGACCGTT ACCCTCCTGG GCAAACGACG CCCACGTACG GCCCACGTCG CCCTTCAATG 300 TCTCTCTTGA CCAATAGGCA ATGCCGGCGA GTTGACAAGG GCCAGTGGGG GCCGGCGGAC 360 AGGGGAAGGA CCCCTGTCGC TGCCCTTCCC GGAGGGACGG GAAATGCATG GGGCCACCCA 420 GCTCCGCGGC GGCCTACAGC CGGGGTAGCC CAAGAACCTT CGGGTGAGGG CGGGTGGCAT 480 TTCTTTTCCT ATACCAATCA YGGCAGTCCT TCTGCTCTTA CTCGTTGTGG AGGCCGGGGC 540 CATYCTGGCC CCGGCCACCC ATGCTTGTAG CGCTAAAGGG CAATATTTC 589 (2) INFORMATION FOR SEQ ID N0:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 589 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID Nθ : 2 : ACCGACGCCT ATCAAAGTAG ACGTGATGGC CCCGCGCCAA ACTGGCGACC GGCCAAAAGG 6 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCATCCT GGTAGCCACT 12 ATAGGTGGGT CTTAAAGGGA GGCTACGGTC CCTCTCGCGC TTATGGAGAG AAAGCGCACG 18 GTCCACAGGT GTTGGCCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC 24 CGAGACCGTT ACCCCTCTGG GCAAACGACG CCCACGTACG GCCCACGTCG CCCTTCAATG 30 TCTCTCTTGA CCAATAGGCA ATGCCGGCGA GTTGACAAGG GCCAGTGGGG GCCGGGCGGT 36 GGGGGAAGGA CCCCCACCGC TGCCCTTCCC GAGGGGGCGG GAAATGCATG GGGCCACCCA 42 GCTCCGCGGC GGCCTACAGC CGGGGTAGTC CAAGAACCTT CGGGTGAGGA CGGGTGGCAT 48 TTCTTTCCTT ATACCGATCA TGGCAGTCTT TCTGCTCTTA CTCGTGGTGG AGGCCGGGGC 540 CATTCTGGCC CCGGCCACAC ATGCTTGTAG TGCTAAAGGG CAATACTTC 589 (2 ) INFORMATION FOR SEQ ID NO : 3 : (i) SEQUENCE CHARACTERISTICS : (A) LENGTH : 591 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID Nθ : 3 : ACCGACGCCT ATCGAAGTAG ACGTGATGGC CCCGCGCCGA ACCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGGA GGTAACGGTC CCTCTCGCGC TTGTGGAGAG AAAGCGCACG 180 GTCCACAGGT GTTGGTCCTA CCGGTGTGAA TAAGGACCCG GCGTTAGGCT CGCCGTTAAA 240 CCGAGCCCGT TAACCCCCTG GGCAAACGAC GCCCATGTAC GGCCTACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC AATGCCGGCG AGTTGACAAG GGCCAGTGGG GGCCGGGCGG 360 AGGGGGAAGG ACCTCCTCCG CAGCCCTTCC CGGGGGTGCG GGAAATGCAT GGGGCCACCC 420 AGCTCCGCGG CGGCCTACAG CCGGGGTAGC CCAAGAGCCT TCGGGTGAGG GCGGGTGGCA 480 TTTTCTCTTC CTATACCGAT CATGGCAGTC CTTCTGCTCT TCTTCGTTGT GGAGGCCGGG 540 GCCATTCTGG CCCCGGCCAC ACACGCTTGT AGCGCAAAGG GGCAATACTT C 591 ( 2 ) INFORMATION FOR SEQ ID Nθ : 4 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 589 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 4 : ACCGACGCCT ACTGAAGTAG ACGCAATGGC CCCGCGCCGA ACCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGGA AGCAAAGGTC CCTCTTGTGC CTGTGGAGGG AACGCGCACG 180 GTCCACAGGT GTTGGTCCTA CCGGTGTAAT AAGGACCCGG CGCTAGGCAC GCCGTTAAAC 240 CGAGTCCGTT ATCCCCCTGG GCAAACGACG CCCATGTACG GCCTACGTCG CCCTTCAATG 300 TCTCTCTTGA CCAATAGGCT TAGCCGGCGA GTTGGCAAGG GCCAGTGGGG GCCGGGCGGG 360 GGGGGAAGGA CCCCCCTCGC TGCCCTTCCC GGGGGAGCGG AAAATGCATG GGGCCACCCA 420 GCTCCGCGGC GGCCTACAGC CGGGGTAGCC CAAGAGCCTT CGGGTGAGGG CGGGTGGCAT 480 TTCTTTTCCT ATACCGATCA TGGCAGTCCT TCTGCTCTTA CTCGTTGTGG AGGCCGGGGC 540 CATCTTGGCC CCGGCCACCC ATGCTTGTAG CGCGAAGGGG CAATATTTC 589 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 591 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GCTGGCCTTA CCGGTGCAAA TAAAGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT TACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 TTGGGGAAGG ACCCCAAGCC CTGCCCTTCC CGGTGGGCCG GGAAATGCAT GGGGCCACCC 420 AGCTCCGCGG CGGCCTGCAG CCGGGGTAGC GCAAGAATCC TTCGGGTGAG GGCGGGTGGC 480 ATTTTTCTTT TCTATACCAT CATGGCAGTC CTTCTGCTCC TTCTCGTGGT TGAGGCCGGG 540 GCCATCCTGG CCCCGGCCAC CCACGCGTGT CGAGCGAATG GGCAATATTT C 591 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 589 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 6 : ACCGACGCCT ACTAAAGTAG ACGCAATGGC TCAGCGCCGA ACCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCATCCT GGTAGCCATT 120 ATAGGTGGGT CTTAAGGGGA AGCAAAGATC CCTCTTGCGC TTATGGAAGG AAAGCGCACG 180 GTCCACAGGT GTTGGCCTAC CGGTGTAATA AGGGCCCGGC GATAGGCACG CCGTTAAACC 240 GAGACCGTTA TCCCTCTGGG CAAACGACGC TCACGTACGG TCCACGTCGC CCTTCAATGC 300 CTCTCTTGGC CAATAGGTTT ATCCGGCGAG TTGACAAGGA CCAGTGAGGG CCGGGCAGGA 360 GGGGGAGGGA CCCCCACTGC CGCCCTTCCC GAGGGAGCGG GAAATGCATG GGGCCACCCA 420 GCTCCGCGGC GGCCTACAGC CGGGGTAGCC CAAGAGCCTT CGGGTGAGGG CGGGTGGCAT 480 TTTTCTTCCT ATACCGATCA TGGCAGTCCT TCTGCTTCTA CTCGTTGTGG AGGCCGGGGC 540 CATTCTGGCC CCGGCCACCC ATGCTTGTAG CGCGAAGGGG CAATATTTC 589 ( 2 ) INFORMATION FOR SEQ ID NO : 7 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 593 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID Nθ : 7 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCAA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT CACCCACCTG GGCAAACGTC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTAGCCGGCC GAGTTGACAA GGACCAGTGG GGGTCGGGGG 360 CTTGGGGAGG GACCCCAAGT CCTGCCCTTC CCGGTGGGCC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAATC CTTCGGGTGA GGGCGGGTGG 480 CATTTTCTCT TTTCTATACC ATCATGGCAG TCCTTCTGCT CCTTCTCGTG GTCGAGGCCG 540 GGGCCATTCT GGCCCCGGCC ACCCACGCTT GTCGAGCGAA TGGGCAATAC TTC 593 ( 2 ) INFORMATION FOR SEQ ID NO : 8 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 590 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: ACCGACGCCT ATCTAAGTAG ACGCGATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTCGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCGCAGGT GTAGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT CACCCACCTG GGCTAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGC 360 TTGGAGAAGG ACTCCAAGTC CTGCCCTTCC CGGTGGGCCG GGAAATGCAT GGGGCCACCC 420 AGCTCCGCGG CGGCCTGCAG CCGGGGTAGC CCAAGAACCT TCGGGTGAGG GCGGGTGGCA 480 TTTCTTTTTC CTATACCATC ATGGCAGTCC TTCTGCTCTT TCTCGTGGTT GAGGCCGGGG 540 CCATTTTGGC CCCGGCCACC CACGCTTGTC GAGCGAATGG GCAATATTTC 590 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 592 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCATCCT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTCAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGT TTATCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 TACGGGGAAG GACCCCGGAC CCCGCCCTTC CCGGTGGGCC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAATC CTTCGGATGA GGGCGGGTGG 480 CATTTCTCTT TTCTATACCA TCATGGCAGT CCTTCTGCTC CTTCTCGTGG TTGAGGCCGG 540 GGCCATTCTG GCCCCGGCCA CCCACGCTTG TCGAGCGAAC GGGCAATATT TC 592 ( 2 ) INFORMATION FOR SEQ ID NO : 10 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 592 base pairs (B) TYPE : nucleic acid ( C) STRANDEDNESS : single ( D) TOPOLOGY : l inear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRI PTION : SEQ ID NO : 10 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCATCCT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTCAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGT TTATCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 TACGGGGAAG GACCCCGGAC CCCGCCCTTC CCGGTGGGCC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAATC CTTCGGATGA GGGCGGGTGG 480 CATTTCTCTT TTCTATACCA TCATGGCAGT CCTTCTGCTC CTTCTCGTGG TTGAGGCCGG 540 GGCCATTCTG GCCCCGGCCA CCCACGCTTG TCGAGCGAAC GGGCAATATT TC 592 (2 ) INFORMATION FOR SEQ ID NO : 11 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 592 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( i i ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : ll : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACATCAGGCT TGTCGTTAAA 240 CCGAGCCCGT CATCCGCCTG GGCTAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGT TCATCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 TATGGGGAAG GACCCCAAAC CCTGCCCTTC CCGGCGGACC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAATC CTTCGGGTGA GGGCGGGTGG 480 CATTTTCCTT TTCTATACCA TCATGGCAGT CCTTCTGCTC CTTCTCGTGG TTGAGGCCGG 540 GGCCATTCTG GCCCCGGCCA CCCACGCTTG TCGAGCGAAT GGGCAATATT TC 592 ( 2 ) INFORMATION FOR SEQ ID NO : 12 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 591 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 12 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GCTGGCCTTA CCGGTGCAAA TAAAGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT TACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 TTGGGGAAGG ACCCCAAGCC CTGCCCTTCC CGGTGGGCCG GGAAATGCAT GGGGCCACCC 420 AGCTCCGCGG CGGCCTGCAG CCGGGGTAGC GCAAGAATCC TTCGGGTGAG GGCGGGTGGC 480 ATTTTTCTTT TCTATACCAT CATGGCAGTC CTTCTGCTCC TTCTCGTGGT TGAGGCCGGG 540 GCCATCCTGG CCCCGGCCAC CCACGCGTGT CGAGCGAATG GGCAATATTT C 591 (2) INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 591 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCAA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGGGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT CACCCACCTG GGCAAACGAC GCCCACGTAT GGTCCACGTC GCCCTTCAAT 300 GCCTCTCTTG GCCAATAGGT TTATCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 TCGGGGAAGG ACCTCGTACC CTGCCCTTCC CGGTGGAACG GGAAATGCAT GGGGCCACCC 420 AGCTCCGCGG CGGCCTGCAG CCGGGGTAGC CCAAGAACCC TTTGGGTGAG GGCGGGTGGC 480 ATATCTTTTT CCTATACCAT CATGGCAGTC CTTCTGCTCC TTTTCGTGGT TGAGGCCGGG 540 GCCATTCTGG CCCCGGCCAC CCACGCTTGT CGAGCGAACG GGCAATATTT C 591 (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 593 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCACCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGCCCGT TACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGA TTCGTCCGGC GAGTTGACAA GGACCAGTGG GGGCCGGGGG 360 TCATGGGGAA GGACCCCAGA CCCTGCCCTT CCCGGTGGGG CGGGAAATGC ATGGGGCCAC 420 CCAGCTCCGC GGCGGCCTGC AGCCGGGGTA GCCCAAAAAA CCTTCGGGTG AGGGCGGGTG 480 GCATTTCTTT TTCCTATACC ATCATGGCAG TCCTTCTGCT CTTTCTCGTG GTGGAGGCCG 540 GGGCCATCTT GGCCCCGGCC ACCCATGCTT GTCGAGCGAA TGGGCAATAT TTC 593 ( 2 ) INFORMATION FOR SEQ ID NO : 15 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 590 base pairs (B) TYPE : nucleic acid ( C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRI PTION : SEQ ID NO : 15 : ACCGACGTCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG AACACGCACG 180 GTCCACAGGT GGTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CTGAGCCCGC ACCCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCCCTTG ACCAATAGGT TTATCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGC 360 CTGGGAAGGA CCCCAGTCCC TGCCCTTCCC GGTGGGACGG GAAATGCATG GGGCCACCCA 420 GCTCCGCGGC GGCCTGCAGC CGGGGTAGCC CAAGAATCCT TCGGGTGAGG GCGGGTGGCΛ 480 TTTTTCTTTT CTATACCATC ATGGCGGTCC TTCTGCTCTT TCTCGTGGTT GAGGCCGGGG 54 0 CCATTCTGGC CCCGGCCACC CACGCTTGTC GAGCGAATGG GCAATATTTC 590 ( 2 ) INFORMATION FOR SEQ ID NO : 16 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 593 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 16 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGACCGA CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGG CTTTGCCCGG CGAGTTGACA AGGACCAGTG GGGGCCGGGG 360 GCAGGGGGAA GGACCCCCTC GCTCCGCCCT TCCCGGTGGG ACGGGAAATG CATGGOGCCA 420 CCCAGCTCCG CGGCGGCCTG CAGCCGGGGT AGCCCAAGAG CCTTCGGGTG AGGGCGGGTG 480 GCATTCTTTT CCTTATACCG ATCATGGCAG TCCTTCTGCT TCTCTTCGTG GTTGAGGCCG 540 GGGCCATTCT GGCCCCGGCC ACCCACGCTT GTCGAGCTGA TGGGCAATAT TTC 593 ( 2 ) INFORMATION FOR SEQ ID NO : 17 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 593 base pairs ( B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 17 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGACCGA CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 3 00 GTCTCTCTTG ACCAATAGGG CTTTGCCCGG CGAGTTGACA AGGACCAGTG" GGGGCCGGGG 360 GCAGGGGGAA GGACCCCCTC GCTCCGCCCT TCCCGGTGGG ACGGGAAATG CATGGGGCCA 420 CCCAGCTCCG CGGCGGCCTG CAGCCGGGGT AGCCCAAGAG CCTTCGGGTG AGGGCGGGTG 480 GCATTCTTTT CCTTATACCG ATCATGGCAG TCCTTCTGCT TCTCTTCGTG GTTGAGGCCG 540 GGGCCATTCT GGCCCCGGCC ACCCACGCTT GTCGAGCTGA TGGGCAATAT TTC 593 (2) INFORMATION FOR SEQ ID NO:IB: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 593 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTCAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGAT CGTCGTTAAA 240 CTGAGACCGA CACCCACCTG GGCAAACGAC GCCCATGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGG CGTTGCCCGG CGAGTTGACA AGGACCAGTG GGGGCCGGGG 360 GCAGGGGGAA GGACCCCCTC GCTCCGCCCT TCCCGGTGGG ACGGGAAATG CATGGGGCCA 420 CCCAGCTCCG CGGCGGCCTG CAGCCGGGGT AGCCCAAGAA CCTTCGGGTG AGGGCGGGTG 480 GCATTCTTCT TCTTATACCG ATCATGGCAG TCCTTCTGCT TTTCTTCGTG GTTGAGGCCG 540 GGGCCATTCT GGCCCCGGCC ACCCACGCTT GTCGAGCTGA TGGGCAATAT TTC 593 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 592 base pairs- (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 19 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGGGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGACCGA CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 GGAGGGAAGG ACCCTCTCAC CCTGCCCTTC CCGGTGGGAC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAGCC TTCGGGTGAG GGCGGGTGGC 480 ATTTTTCTTT TCTATACCGA TCATGGCAGT CCTTCTGCTC TTCTTCGTGG TTGAGGCCGG 540 GGCCATTCTG GCCCCGGCCA CCCACGCTTG CCGAGCTGAT GGACAATATT TC 592 ( 2 ) INFORMATION FOR SEQ ID NO : 20 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 591 base pairs ( B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : l inear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 20 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 24 0 CTGAGACCGA CACCCACCTG GGCAAACGAC GCCCATGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGGGT 360 GGGGGGAAGG ACCCCCCACC CTGCCCTTCC CGGTGGGACG GGAAATGCAT GGGGCCACCC 420 AGCTCCGCGG CGGCCTGCAG CCGGGGTAGC CCAAGAGCCT TCGGGTGAGG GCGGGTGGCA 480 TTTTCCTCTT TCATACCGAT CATGGCAGTC CTTCTGCTCT TCTTCGTGGT TGAGGCCGGG 54 0 GCCATTCTGG CCCCGGCCAC CCACGCTTGT CGGGCTGATG GGCAATATTT C 591 (2) INFORMATION FOR SEQ ID NO: 21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 592 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCTGTTCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGACCGA CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGTGC 360 TGGGGGAAGG ACCCCCTTGC ACCGCCCTTC CCGGTGGGAC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAGCC TTCGGGTGAG GGCGGGTGGC 480 ATTTCTCTTT CCCTGACTAA TCATGGCAGT CCTTCTGCTC TTCTTCGTGG TTGAGGCCGG 540 GGCCATTCTG GCCCCGGCCA CCCACGCTTG TCGGGCGAAT GGGCAATACT TC 592 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 591 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 1 CCGAGACCGA CACCCACCTG GGCAAACGAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG GACCAGTGGG GGCCGGGTGC 360 TGGGGGAAGG ACCCCCTTGC ACCGCCCTTC CCGGTGGGAC GGGAAATGCA TGGGGCCACC 420 CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAGCC TTCGGGTGAG GGCGGGTGGC 480 ATTTTCTTTT CCTGACTAAT CATGGCAGTC CTTCTGCTCT TCTTCGTGGT TGAGGCCGGG 540 GCCATTCTGG CCCCGGCCAC CCACGCTTGT CGGGCGAATG GGCAATACTT C 591 (2) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 592 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 23 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGACCGA CACCCACCTG GGCAAACGAT CGCCCACGTA CGGTCCACGT CGCCCTTCAA 300 TGTCTCTCTT GACCAATAGG CTTTGCCGGC GAGTTGACAA GGACCAGTGG GGGCCGGGGG 360 CTGGGAGAAG GACTCCCACG CCCCGCCCTT CCCGGGGGGA CGGGAAATGC ATGGGGCCAC 420 CCAGCTCCGC GGCGGCCTGC AGCCGGGGTA GTCCAAGAGC CTTCGGGTGA GGACGGGTGG 480 CATTTCTTTT TCTACACCGA TCATGGCAGT CCTTCTGCTC TTCTTCGTGG TTGAGGCCGG 540 GGCCATTCTG GCCCCGGCCA CCCACGCTTG TCGAGCTGGT GGGCAATATT TC 592 ( 2 ) INFORMATION FOR SEQ ID NO : 24 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 593 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear (ii) MOLECULE TYPE: DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 24 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CCCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGGGAA GGTTAAGATT CCTCTTGTGC CTGTGGCGAG ACAGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGGGAA TAAGGGCCCG ACGTCAGGCT CGTCGTTAAA 240 CCGAGACCGA CCCCCACCTG GGCAAACGGC GCTCATGTAC GGTCCACGTC GCCCTTCAAT 300 GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG GACCAGTGAG GGCCGGGGGC 360 AGGGGGGAGG GACCCCCCTG TCCCGCCCTT CCCGGTGGGA CGGGAAATGC ATGGGGCCAC 420 CCAGCTCCGC GGCGGCCTGC AGCCGGGGTA GCCCAAGAAA CCTTCGGGTG AGGGCGGGTG 480 GCATTTTCTT TTCTATACCA ATCATGGCAG TCCTTCTGCT CTTCTTCGTG GTTGAGGCCG 540 GGGCCATCTT GGCCCCGGCC ACCCACGCTT GTCGGGCTGA TGGGCAATAT TTC 593 ( 2 ) INFORMATION FOR SEQ ID NO : 25 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 595 base pairs (B ) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 25 : ACCGACGCCT ATCTAAGTAG ACGCAATGAC TCGGCGCCGA CTCGGCGACC GGCCAAAAGG 60 TGGTGGATGG GTGATGCCAG GGTTGGTAGG TCGTAAATCC CGGTCATCTT GGTAGCCACT 120 ATAGGTGGGT CTTAAGAGAA GGTTAAGATT CCTCTTGTGC CTGCGGCGAG ACCGCGCACG 180 GTCCACAGGT GTTGGCCCTA CCGGTGTAAT AAGGGCCCGA CGTCAGGCTC GTTGCTTAAA 240 CCGAAGCCCG TCACCCACCT GGCAGCGAAC GCCCACGTAC GGTCCACGTC GCCCTTCAAT 300 GCCTCTCTTG GCCAATAGGA GATTCCTCGG CGAGTTGGCA AGGACCAGTG GGGGCCGGGG 360 GTCACAGGGA AGGACCCTGG ACCCTGCCCA TCCCGGTGGG CCGGGAAATC GATGGGGCCA 42 CCCAGCTCCG CGGCCCGGCC TGCAGCCGGG GTAGCCCAAG AATCTTCGGG TGAGGGCGGG 48 TGGCATTTCT CTTTCCTATA CCATCATGGC AGTCCTTCTG CTCCTTCTCG TGGTTGAGGC 54 CGGGGCCATT CTGGCCCCGG CCACCCACGC TTGTAGAGCG AATGGGCAAT ATTTC 595 (2 ) INFORMATION FOR SEQ ID NO : 6 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 9126 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 26 : CCCCCCCCCC GGCACTGGGT GCAAGCCCCA GAAACCGACG CCTACTGAAG TAGACGTAAT 60 GGCCCCGCGC CGAACCGGCG ACCGGCCAAA AGGTGGTGGA TGGGTGATGA CAGGGTTGGT 120 AGGTCGTAAA TCCCGGTCAT CCTGGTAGCC ACTATAGGTG GGTCTTAAGG GGAGGCTACG 180 GTCCCTCTTG CGCATATGGA GGAAAAGCGC ACGGTCCACA GGTGTTGGTC CTACCGGTGT 240 AATAAGGACC CGGCGCTAGG CACGCCGTTA AACCGAGCCC GTTACTCCCC TGGGCAAACG 300 ACGCCCACGT ACGGTCCACG TCGCCCTTCA ATGTCTCTCT TGACCAATAG GCGTAGCCGG 360 CGAGTTGACA AGGACCAGTG GGGGCCGGGC GGGAGGGGGA AGGACCCCCA CCGCTGCCCT 420 TCCCGGGGAG GCGGGAAATG CATGGGGCCA CCCAGCTCCG CGGCGGCCTA CAGCCGGGGT 480 AGCCCAAGAA CCTTCGGGTG AGGGCGGGTG GCATTTCTTT TCCTATACCG ATCATGGCAG 540 TCCTTCTGCT CCTACTCGTG GTGGAGGCCG GGGCTATTTT AGCCCCGGCC ACCCATGCTT 600 GTAGCGCGAA AGGGCAATAT TTBCTCACAA ACTGTTGCGC CCTGGAGGAC ATAGGCTTCT 660 GCCTGGAGGG CGGATGCCTG GTGGCTCTGG GGTGCACCAT TTGCACCGAC CGCTGCTGGC 720 CACTGTATCA GGCGGGTTTG GCCGTGCGGC CCGGCAAGTC CGCCGCCCAG TTGGTGGGGG 780 AACTCGGTAG TCTCTACGGG CCCTTGTCGG TCTCGGCTTA TGTGGCCGGG ATCCTGGGGC 840 TTGGGGAGGT CTACTCGGGG GTCCTCACCG TCGGGGTGGC GTTGACGCGC AGGGTCTACC 900 CGGTCCCGAA CCTGACGTGT GCAGTAGAGT GTC-AGTTGAA GTGGGAAAGT GAGTTTTGGΛ 960 GATGGACTGA ACAGCTGGCC TCAAACTACT GGATTCTGGA ATACCTCTGG AAGGTGCCTT 1020 TCGACTTTTG GCGGGGAGTG ATGAGCCTTT CTCCTCTCTT GGTGTGCGTG GCGGCCCTCC 1080 TCCTGCTGGA GCAGCGTATT GTCATGGTCT TCCTCCTGGT CACTATGGCG GGCATGTCAC 1140 AAGGCGCGCC CGCCTCAGTG TTGGGGTCAC GGCCTTTCGA GGCCGGGCTG ACTTGGCAGT 1200 CTTGTTCTTG CAGGTCGAAC GGGTCCCGCG CGCCGΛCΛGG GGAGAGGGT TGGGAΛCGTG 1260 GGAACGTCAC ACTTTTGTGT GACTGCCCCA ACGGTCCTTG GGTGTGGGTC CCGGCCCTTT 1320 GCCAGGCAAT CGGATGGGGC GACCCTATCA CTCATTGGAG CCACGGACGA AATCAGTGGC 1380 CCCTTTCTTG TCCCCAATTT GTCTACGGCG CCGTTTCAGT GACCTGCGTG TGGGGTTCTG 1440 TGTCTTGGTT TGCTTCCACT GGGGGTCGCG ACTCCAAGGT TGATGTGTGG AGTTTGGTTC 1500 CAGTTGGCTC TGCCAGCTGT ACCATAGCCG CACTGGGATC TTCGGATCGC GACACAGTGG 1560 TTGAGCTCTC CGAATGGGGA ATCCCCTGCG CCACTTGTAT CCTGGACAGG CGGCCTGCCT 1620 CGTGTGGCAC CTGTGTGAGG GACTGCTGGC CCGAGACCGG GTCGGTACGT TTCCCATTCC 1680 ACAGGTGTGG CGCGGGACCG AGGCTGACCA GAGACCTTGA GGCTGTGCCC TTCGTCAATA 1740 GGACAACTCC CTTCACCATA AGGGGGCCCC TGGGCAACCA GGGGCGAGGC GACCCGGTGC 1800 GGTCGCCCTT GGGTTTTGGG TCCTACACCA TGACCAAGAT CCGAGACTCC TTACACTTGG 1860 TGAAATGTCC CACCCCAGCC ATTGAGCCTC CCACCGGAAC GTTTGGGATC TTCCCAGGAG 1920 TCCCCCCCCT TAACAΛCTGC ΛTGCTTCTCG GCΛCTGΛGGT GTCΛGAGGTΛ TTGGGTGGGG 1 80 CGGGCCTCAC TGGGGGGTTT TACGAACCTC TGGTGCGGCG GTGTTCAGAG CTGATGGGTC 2040 GGCGGAATCC GGTCTGCCCG GGGTTTGCAT GGCTCTCTTC GGGACGGCCT GATGGGTTCA 2100 TACATGTACA GGGCCACTTG CAGGAGGTGG ATGCGGGCAA CTTCATTCCG CCCCCACGCT 2160 GGTTGCTCTT GGACTTTGTA TTTGTCCTGT CATACCTGAT GAAGCTGGCA GAGGCACGGT 2220 TGGTCCCGCT GATCCTCCTC CTGCTATGGT GGTGGGTGAA CCAGTTGGCG GTCCTTGKAC 2280 TGSCGGCTGC KCRCGCCGCC GTGGCTGGAG AGGTGTTTGC GGGCCCTGCC TTGTCCTGGT 2340 GTCTGGGCCT ACCCTTCGTG AGTATGATCC TGGGGCTAGC AAACCTGGTG TTGTACTTCC 2400 GCTGGATGGG TCCTCAACGC CTGATGTTCC TCGTGTTGTG GAAGCTCGCT CGGGGGGCTT 2460 TCCCGCTGGC ATTACTGATG GGGATTTCCG CCACTCGCGG CCGCACCTCT GTGCTTGGCG 2520 CCGAATTCTG CTTTGATGTC ACCTTTGAAG TGGACACGTC AGTCTTGGGT TGGGTGGTTG 2580 CTAGTGTGGT GGCTTGGGCC ATAGCGCTCC TGAGCTCTAT GAGCGCGGGG GGGTGGAAGC 2640 ACAAAGCCAT AATCTATAGG ACGTGGTGTA AAGGGTACCA GGCYCTTCGC CAGCGCGTGG 2700 TGCGTAGCCC CCTCGGGGAG GGGCGGCCCA CCAAGCCGCT GACGATAGCC TGGCGTCTGG 2760 CCTCTTACAT CTGGCCGGAC GCTGTGATGT TGGTGGTTGT GGCCATGGTC CTCCTCTTCG 2820 GCCTTTTCGA CGCGCTCGAT TGGGCCTTGG AGGAGCTCCT TGTGTCGCGG CCTTCGTTGC 2880 GTCGTTTGGC AAGGGTGGTG GAGTGTTGTG TGATGGCGGG CGAGAAGGCC ACTACCGTCC 2940 GGCTTGTGTC CAAGATGTGC GCGAGAGGGG CCTACCTGTT TGACCACATG " GGGTCGTTCT 3000 CGCGCGCGGT CAAGGAGCGC TTGCTGGAGT GGGACGCGGC TTTGGAGMCC CTGTCATTCA 3060 CTAGGACGGA CTGCCGCATC ATACGAGACG CCGCCAGGAC TCTGAGCTGC GGCCAATGCG 3120 TCATGGGCTT GCCCGTGGTG GCTAGGCGCG GCGATGAGGT CCTGGTTGGG GTCTTTCAGG 3180 ATGTGAACCA CTTGCCTCCG GGGTTTGYTC CTACAGCGCC TGTTGTCATC CGTCGGTGCG 3240 GAAAGGGCTT CCTCGGGGTC ACTAAGGCTG CCTTGACTGG TCGGGATCCT GACTTACACC 3300 CAGGAAACGT CATGGTTTTG GGGACGGCTA CCTCGCGCAG CATGGGAACG TGCTTAAACG 3360 GGTTGCTGTT CACGACATTC CATGGGGCTT CTTCCCGAAC CATTGCGACA CCTGTGGGGG 3420 CCCTTAACCC AAGGTGGTGG TCGGCCAGTG ATGACGTCAC GGTCTATCCC CTCCCCGATG 3480 GAGCTAACTC GTTGGTTCCC TGCTCGTGTC AGGCTGAGTC CTGTTGGGTC ATYCGATCCG 3540 ATGGGGCTCT TTGCCATGGC TTGAGCAAGG GGGACAAGGT AGAACTGGAC GTGGCCATGG 3600 AGGTTGCTGA CTTTCGTGGG TCGTCTGGGT CTCCTGTCCT ATGCGACGAG GGGCACGCTG 3660 TAGGAATGCT CGTGTCCGTC CTTCATTCGG GGGGGAGGGT GACCGCGGCT CGATTCACTC 3720 GGCCGTGGAC CCAAGTCCCA ACAGACGCCA AGACTACCAC TGAGCCACCC CCGGTGCCAG 3780 CTAAAGGGGT TTTCAAAGAG GCTCCTCTTT TCATGCCAAC AGGGGCGGGG AAAAGCACAC 3840 GCGTCCCTTT GGAATATGGA AACATGGGGC ACAAGGTCCT GCTTCTCAAC CCGTCGGTTG 3900 CCACTGTGAG GGCCATGGGC CCTTACATGG AGAAGCTGGC GGGGAAACAT CCTAGCATTT 3 960 TCTGTGGACA CGACACAACA GCTTTCACAC GGATCACGGA CTCTCCATTG ACGTACTCTA 4020 CCTATGGGAG GTTTCTGGCC AACCCGAGGC AGATGCTGAG GGGAGTTTCC GTGGTCATCT 4080 GTGATGAGTG CCACAGTCAT GACTCAACTG TGTTGCTGGG TATAGGCAGG GGCAGGGAGC 4140 TGGCGCGGGG GTGTGGAGTG CAATTAGTGC TCTACGCTAC TGCGACTCCC CCGGGCTCGC 4200 CTATGACTCA GCATCCATCC ATAATTGAGA CAAAGCTGGA CGTCGGTGAG ATCCCCTTTT 4260 ATGGGCATGG TATCCCCCTC GAGCGTATGA GGACTGGTCG CCACCTTGTA TTCTGCCATT 4320 CCAAGGCGGA GTGCGAGAGA TTGGCCGGCC AGTTCTCCGC GCGGGGGGTT AATGCCATCG 4380 CCTATTATAG GGGTAAGGAC AGTTCCATCA TCAAAGACGG AGACCTGGTG GTTTGTGCGA 4440 CAGACGCGCT CTCTACCGGG TACACAGGAA ACTTCGATTC TGTCACCGAC TGTGGGTTAG 4500 TGGTGGAGGA GGTCGTTGAG GTGACCCTTG ATCCCACCAT TACCATTTCC TTGCGGACTG 4560 TCCCTGCTTC GGCTGAATTG TCGATGCAGC GGCGCGGACG CACGGGGAGA GGTCGGTCGG 4620 GCCGCTACTA CTACGCTGGG GTCGGTAAGG CTCCCGCGGG GGTGGTGCGG TCTGGTCCGG 4680 TCTGGTCGGC AGTGGAAGCT GGAGTGACCT GGTATGGAAT GGAACCTGAC TTGACAGCAA 4740 ACCTTCTGAG ACTTTACGAC GACTGCCCTT ACACCGCAGC CGTCGCAGCT GACATTGGTG 4800 AAGCCGCGGT GTTCTTTGCG GGCCTCGCGC CCCTCAGGAT GCATCCCGAT GTTAGCTGGG 4860 CAAAAGTTCG CGGCGTCAAT TGGCCCCTCC TGGTGGGTGT TCAGCGGACG ATGTGTCGGG 4920 AAACACTGTC TCCCGGCCCG TCGGACGACC CTCAGTGGGC AGGTCTGAAA GGCCCGAATC 4980 CTGCCCCACT ACTGCTGAGG TGGGGCAATG ATTTGCCATC AAAAGTGGCC GGCCACCACA 5040 TAGTTGACGA TCTGGTCCGT CGGCTCGGTG TGGCGGAGGG ATACGTGCGC TGTGATGCTG 5100 GRCCCATCCT CATGGTGGGC TTGGCCATAG CGGGCGGCAT GATCTACGCC TCTTACACTG 5160 GGTCGCTAGT GGTGGTAACA GACTGGAATG TGAAGGGAGG TGGCAATCCC CTTTATAGGA 5220 GTGGTGACCA GGCCACCCCT CAACCCGTGG TGCAGGTCCC CCCGGTAGAC CATCGGCCGG 5280 GGGGGGAGTC TGCGCCAGCG GATGCCAAGA CAGTGACAGA TGCGGTGGCA GCCATCCAGG 5340 TGAACTGCGA TTGGTCTGTG ATGACCCTGT CGATCGGGGA AGTCCTCACC TTGGCTCAGG 5400 CTAAGACAGC CGAGGCCTAC GCAGCTACTT CCAGGTGGCT CGCTGGCTGC TACACGGGGΛ 5460 CGCGGGCCGT CCCCACTGTA TCAATTGTTG ACAAGCTCTT CGCCGGGGGT TGGGCCGCCG 5520 TGGTGGGTCA CTGTCACAGC GTCATTGCTG CGGTGGTGGC TGCCTATGGG GTTTCTCGAA 5580 GTCCTCCACT GGCCGCGGCG GCATCCTACC TCATGGGGTT GGGCGTCGGA GGCAACGCAC 5640 AGGCGCGCTT GGCTTCAGCT CTTCTACTGG GGGCTGCTGG TACGGCTCTG GGGACCCCTG 5700 TCGTGGGACT CACCATGGCG GGGGCCTTCA TGGGCGGTGC CAGCGTGTCC CCCTCCCTCG 5760 TCACTGTCCT ACTTGGGGCT GTGGGAGGTT GGGAGGGCGT TGTCAACGCT GCCAGTCTCG 5820 TCTTCGACTT CATGGCTGGG AAACTTTCAA CAGAAGACCT TTGGTATGCC ATCCCGGTAC 5880 TCACTAGTCC TGGRGCGGGC CTCGCGGGGA TTGCCCTTGG TCTGGTTTTG TACTCAGCAA 5940 ACAACTCTGG CACTACCACA TGGCTGAACC GTCTGCTGAC GACGTTGCCA CGGTCATCTT 6000 GCATACCCGA CAGCTACTTC CAACAGGCTG ACTACTGCGA CAAGGTCTCG GCAATGCTGC 6060 GCCGCCTGAG CCTTACTCGC ACCGTGGTGG CCCTGGTCAA CAGGGAGCCT AAGGTGGATG 6120 AGGTCCAGGT GGGGTACGTC TGGGATCTGT GGGAGTGGGT AATGCGCCAG GTGCGCATGG 6180 TGATGTCTAG ACTCCGGGCC CTCTGCCCTG TGGTGTCACT CCCCTTGTGG CACCGCGGGG 6240 AGGGGTGGTC CGGTGAATGG CTTCTCGATG GGCACGTGGA GAGTCGTTGT CTGTGCGGGT 6300 GTGTAATCAC CGGCGACGTC CTCAATGGGC AACTCAAAGA TCCAGTTTAC TCTACCAAGC 6360 TGTGCAGGCA CTACTGGATG GGAACTGTGC CGGTCAACAT GCTGGGCTAC GGGGAAACCT 6420 CACCTCTTCT CGCCTCTGAC ACCCCGAAGG TGGTACCCTT CGGGACGTCG~ GGGTGGGCTG 6480 AGGTGGTGGT GACCCCTACC CACGTGGTGA TCAGGCGCAC GTCCTGTTAC AAACTGCTTC 65 40 GCCAGCAAAT TCTTTCAGCA GCTGTAGCTG AGCCCTACTA CGTTGATGGC ATTCCGGTCT 6600 CTTGGGAGGC TGACGCGAGA GCGCCGGCCA TGGTCTACGG TCCGGGCCAA AGTGTTACCA 6660 TTGATGGGGA GCGCTACACC CTTCCGCACC AGTTGCGGAT GCGGAATGTG GCGCCCTCTG 672 0 AGGTTTCATC CGAGGTCAGC ATCGAGATCG GGACGGAGAC TGAAGACTCA GAACTGACTG 6780 AGGCCGATTT GCCACCAGCG GCTGCTGCCC TCCAAGCGAT AGAGAATGCT GCGAGAATTC 6840 TCGAACCGCA CATCGATGTC AYCATGGAGG ATTGCAGTAC ACCCTCTCTC TGTGGTAGTA 6900 GCCGAGAGAT GCCTGTGTGG GGAGAAGACA TACCCCGCAC TCCATCGCCT GCACTTATCT 6960 CGGTTACGGA GAGCAGCTCA GATGAGAAGA CCCTGTCGGT GACCTCCTCG CAGGAGGACA 7020 CCCCGTCCTC AGACTCATTT GAAGTCATCC AAGAGTCTGA TACTGCTGAA TCAGAGGAAA 7080 GCGTCTTCAA CGTGGCTCTT TCCGTACTAA AAGCATTATT TCCACAGAGC GTTGCCACAC 7140 GAAAGCTAAC GGTTAAGATG TCTTGCTGTG TTGAGAAGAG CGTAACACGC TTCTTTTCTT 7200 TAGGGTTGAC CGTGGCTGAC GTGGCTAGCC TGTGTGAGAT GGAGATCCAG AACCATACAG 7260 CCTATTGTGA CAAGGTGCGC ACTCCGCTCG AATTGCAAGT TGGGTGCTTG GTGGGCAATG 7320 AACTTACCTT TGAATGTGAC AAGTGTGAGG CACGCCAAGA GACCCTTGCC TCCTTCTCCT 7380 ACATATGGTC CGGGGTCCCA CTTACTCGGG CCACTCCGGC CAAACCACCA GTGGTGAGGC 7440 CGGTGGGGTC CTTGTTGGTG GCAGACACCA CCAAGGTCTA CGTGACCAAT CCGGACAATG 7500 TTGGGAGGAG GGTTGACAAG GTGACTTTCT GGCGCGCTCC TCGGGTACAC GACAAGTTCC 7560 TCGTGGACTC GATCGAGCGC GCTCGGAGAG CTGCTCAAGG CTGCCTAAGC ATGGGTTACA 7620 CTTATGAGGA GGCAATAAGG ACTGTTAGGC CGCATGCTGC CATGGGCTGG GGATCTAAGG 7680 TGTCGGTCAG GGACTTGGCC ACCCCTGCGG GGAAGATGGC TGTTCATGAC CGGCTTCAGG 7740 AGATACTTGA AGGGACTCCA GTCCCTTTTA CCCTGACTGT CAAAAAGGAG GTGTTCTTCA 7800 AAGATCGTAA GGAGGAGAAG GCCCCCCGCC TCATTGTGTT CCCCCCCCTG GACTTCCGGA 7860 TAGCTGAAAA GCTCATTCTG GGAGACCCGG GGCGGGTTGC AAAGGCGGTG TGGGGGGGGG 7920 CTTACGCCTT CCAGTACACC CCCAACCAGC GGGTTAAGGA GATGCTAAAG CTGTGGGAAT 7980 CAAAGAAGAC CCCGTGCGCC ATCTGTGTGG ATGCCACTTG CTTCGACAGT AGCATTACTG 8040 ARGAGGACGT GGCACTAGAG ACAGAGCTTT ACGCCCTGGC CTCGGACCAT CCAGAATGGG 8100 TGCGCGCCCT GGGGAAATAC TRTGCCTCTG GCACAATGGT GACCCCGGAA GGGGTGCCAG 8160 TGGGCGAGAG GTATTGTAGG TCCTCGGGTG TGTTAACCAC AAGTGCTAGC AACTGTTTGA 8220 CCTGCTACAT CAAAGTGAGA GCCGCCTGTG AGAGGATCGG ACTGAAAAAT GTCTCGCTTC 8280 TCATCGCGGG CGATGACTGC TTAATTGTGT GCGAGAGGCC TGTATGCGAC CCTTGCGAGG 8340 CCCTGGGCCG AGCCCTGGCT TCGTACGGGT ACGCGTGTGA GCCCTCGTAT CACGCTTCAC 8400 TGGACACAGC CCCCTTCTGC TCCACTTGGC TTGCTGAGTG CAATGCGGAT GGGRAAAGGC 8460 ATTTCTTCCT GACCACGGAC TTTCGGAGAC CACTCGCTCG CATGTCGAGC GAGTACAGTG 8520 ACCCTATGGC TTCGGCCATT GGTTACATTC TCCTCTATCC CTGGCRTCCC ATCACACGGT 8580 GGGTCATCAT CCCGCATGTG CTAACATGCG CTTCTTTCCG GGGTGGTGGC ACACSGTCTG 8640 ATCCGGTTTG GTGTCAGGTT CATGGTAACT ACTACAAGTT TCCCCTGGAC AAACTGCCTA 8700 ACATCATCGT GGCCCTCCAC GGACCAGCAG CGTTGAGGGT TACCGCAGAC ACAACCAAAA 8760 CAAAGATGGA GGCTGGGAAG GTTCTGAGCG ACCTCAAGCT CCCTGGTCTA GCCGTCCACC 8820 GCAAGAAGGC CGGGGCATTG CGAACACGCA TGCTCCGGTC GCGCGGTTGG GCGGAGTTGG 8880 CTAGGGGCCT GTTGTGGCAT CCAGGACTCC GGCTTCCTCC CCCTGAGATT GCTGGTATCC 8940 CAGGGGGTTT CCCTCTGTCC CCCCCCTACA TGGGGGTGGT TCATCAATTG GATTTCACAG 9000 CSCAGCGGAG TCGCTGGCGG TGGTTGGGGT TCTTAGCCCT GCTCATCGTA GCGCTCTTTG 9060 GGTGAACTAA ATTCATCTGT TGCGGCCGGA GTCAGACCTG AGCCCCGTTC AAAAGGGGAT 9120 TGAGAC 9126 ( 2 ) INFORMATION FOR SEQ ID NO : 27 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 20 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 27 : AAGAGGGACC GTTGCTTCCC 20 (2 ) INFORMATION FOR SEQ ID NO : 28 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 19 base pairs (B) TYPE : nucleic acid ( C) STRANDEDNESS : single. (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28: TTAAGGGGAA GCAACGGTC 19 (2) INFORMATION FOR SEQ ID NO:29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29: TTAAGAGAAG GTTAAGATT 19 (2) INFORMATION FOR SEQ ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30: AGAGGAATCT TAACCTTCT 19 (2) INFORMATION FOR SEQ ID NO:31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs • (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31: TTTCCCTCCA TAAGCGCG 18 (2) INFORMATION FOR SEQ ID NO:32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32: CGCGCTTATG GAGAGAAA 18 (2) INFORMATION FOR SEQ ID NO:33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33 TGTGCCTGYG GCGAGACM (2) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34: KGTCTCGCCR CAGGCACA 18 (2) INFORMATION FOR SEQ ID NO:35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35: CCCAGAAACC GACGCCTATC 20 (2) INFORMATION FOR SEQ ID NO:36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36: ATAGGCGTCG GTTTCTGGG 19 (2) INFORMATION FOR SEQ ID NO:37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37: AAGTAGACGC AATGACTCGG 20 (2) INFORMATION FOR SEQ ID NO:38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38: CCGAGTCATT GCGTCTACTT A 21 (2) INFORMATION FOR SEQ ID NO:39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39: CACTATAGGT GGGTCTTAAG 20 (2) INFORMATION FOR SEQ ID NO:40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 0: TTAAGACCCA CCTATAGTG 19 (2) INFORMATION FOR SEQ ID NO:41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41: GAAGGTTAAG ATTCCTCTTG 20 (2) INFORMATION FOR SEQ ID NO:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42: CAAGAGGAAT CTTAACCTTC T 21 (2) INFORMATION FOR SEQ ID NO:43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43: CGCAATGACT CGGCGCCGA 19 (2) INFORMATION FOR SEQ ID NO:44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44: GGCGCCGAGT CATTGCG 17 (2) INFORMATION FOR SEQ ID NO:45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45: GGCGACCGGC CAAAAGGTG 19 (2) INFORMATION FOR SEQ ID NO:46: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46: CACCTTTTGG CCGGTCGCCG GG 22 (2) INFORMATION FOR SEQ ID NO:47: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47: CCCCGGCCAC CCATGCTTGT A 21 (2) INFORMATION FOR SEQ ID NO:48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48: ACAAGCATGG GTGGCCGGGG 20 (2) INFORMATION FOR SEQ ID NO:49: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49: CGCGAAGGGG CAATATTTC 19 (2) INFORMATION FOR SEQ ID NO:50: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50: GAAATATTGC CCCTTCGCGC 20 (2) INFORMATION FOR SEQ ID NO:51: (i) SEQUENCE ' CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51: CACTGGGTGC AAGCCCCAGA A 21 (2) INFORMATION FOR SEQ ID NO:52: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52: CAGGGCGCAA CAGTTTGTGA G 21 (2) INFORMATION FOR SEQ ID NO:53: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53: AGGGTTGGTA GGTCGTAAAT CCC 23 (2) INFORMATION FOR SEQ ID NO:54: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54: CACTGGTCCT TGTCAACTCG C 21 (2) INFORMATION FOR SEQ ID NO:55: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:55: CCGTACGTGG GCGTCGTTTG C 21 (2) INFORMATION FOR SEQ ID NO:56: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:56: CGGCCAAAAG GTGGTGGATG G 21 (2) INFORMATION FOR SEQ ID NO:57: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57: CGGAGCTGGG TGGCCCCATG C 21 (2) INFORMATION FOR SEQ ID NO:58: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58: CCACAAACAC TCCAGTTTGT TAC 23 (2) INFORMATION FOR SEQ ID NO:59: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59: GCTCTAGACA TGTGCTACGG TCTACGAG 28 (2) INFORMATION FOR SEQ ID NO:60: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 633 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 60 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCTAAGTA GACGCAATGA CTCGGCGCCG 60 ACTCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCACCT TGGTAGCCAC TATAGGTGGG TCTTAAGAGA AGGTTAAGAT TCCTCTTGTG 180 CCTGCGGCGA GACCGCGCAC GGTCCACAGG TGCTGGCCTT ACCGGTGCAA ATAAAGGCCC 240 GACGTCAGGC TCGTCGTTAA ACCGAGCCCG TTACCCACCT GGGCAAACGA CGCCCACGTA 300 CGGTCCACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CTTAGCCGGC GAGTTGACAA 360 GGACCAGTGG GCGCCGGGGG TTTGGGGAAG GACCCCAAGC CCTGCCCTTC CCGGTGGGCC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAATC 480 CTTCGGGTGA GGGCGGGTGG CATTTTTCTT TCCTATACCA TCATGGCAGT CCTTCTGCTC 540 CTTCTCGTGG TTGAGGCCGG GGCCATCCTG GCCCCGGCCA CCCACGCGTG TCGAGCGAAT 600 GGGCAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2) INFORMATION FOR SEQ ID NO: 61: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 366 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 61: AGGGTTGGTA GGTCGTAAAT CCCGGTCACC TTGGTAGCCA CTATAGGTGG GTCTTAAGGG 60 GAGGTTAAGA TTCCTCTTGT GCCTGCGGCG AGACCGCGCA CGGTCCACAG GTGTTGGCCC 120 TACCGGTGTG AATAAGGGCC CGACGTCAGG CTCGTCGTTA AACCGAGCCC GTTCCCCGCC 180 TGGGCAAACG ACGCCCACGT ACGGTCCACG TCGCCCTTCA ATGTCTCTCT TGACCAATAG 240 GTTCATCCGG CGAGTTGACA AGGACCAGTG GGGGCCGGGG GTCATGGGGA AGGACCCCAG 300 ACCCTGCCCT TCCCGGCGGG YCGGGAAATG CATGGGGCCA CCCAGCTCCG GCATGGGGCC 360 ACCCAG 3 66 (2) INFORMATION FOR SEQ ID NO: 62: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 349 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID' O: 62: AGGGTTGGTA GGTCGTAAAT CCCGGTCACC TTGGTAGCCA CTATAGGTGG GTCTTAAGAG 60 AAGGTTAAGA TTCCTCTTGT GCCTGCGGCG AGACCGCGCA CGGTCCACAG GTGTTGGCCC 120 TACCGGTGTT AATAAGGGCC CGACGTCAGG CTCGTCGTTA AACCGAGCCC GTCACCCACC 180 TGGGCAAACG ACGCCCACGT ACGGTCCACG TCGCCCTTCA ATGTCTCTCT TGACCAATAG 240 GCTTAGCCGG CGAGTTGACA AGGACCAGTG GGGGCCGGGG GCCTGGAGAG GGACTCCAGG 300 TCCTGCCCTT CCCGGTGGGC CGGGAAATGC ATGGGGCCAC CCAGCTCCG 349 (2) INFORMATION FOR SEQ ID NO:63: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 63 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATTTAAACA GACGTTATGA ACCGGCGCCG 60 ACCCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGCCA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCT TGGTAGCCAC TATAGGTGGG TCTTAAGGGT TGGTCAAGGT CCCTCTGGCG 180 CTTGTGGCGA GAAAGCGCAC GGTCCACAGG TGTTGGCCCT ACCGGTGTGA ATAAGGGCCC 240 GACGTCAGGC TCGTCGTTAA ACCGAGCCCA TTACCCACCT GGGCAAACGA CGCCCACGTA 300 CGGTCCACGT CGCCCTACAA TGTCTCTCTT GACCAATAGG CTTTGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGCCGGGCG GCAGGGGAAG GACCTCTGTC GCTGCCCTTC CCGGTGGGGT 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTCTTCTTC TTTAACCGAT CATGGCAGTC CTTCTGCTTC 540 TCCTCGTTGT GGAGGCCGGG GCCATTCTGG CCCCGGCCAC ACACGCTTGT GGAGCGAATG 600 GGCAATATTT CCTCACAAAC TGTTGCGCCC TG 632 ( 2 ) INFORMATION FOR SEQ ID NO : 64 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 633 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic ) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 64 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG TCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG- GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGT CCCTCTTGCG 180 CCCATGGAGG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCCG 240 GCGATAGGCA CGCCGTTAAA CTGAGACCGT TACCCCTCTG GGTAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGATG GACCCCCACC GCTGCCCATT CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTTTTCTTT CCTATACCGA TCATGGCAGT CCTTCTGCTC 540 TTACTCGTGG CGGAGGCCGG GGCCATCTTG GCCCCGGCCA CCCATGCTTG TAGTGCGCAG 600 GGACAATATT TCCTCACAAA CTGTTGCGCC CTG 633 ( 2 ) INFORMATION FOR SEQ ID NO : 65 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 632 base pairs (B ) TYPE : nucleic acid ( C) STRANDEDNESS : double (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 65 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG CCCCGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGT CCCTCTTGTG 180 CCTGTGGAGG GAAAGCGCAC GGTCCACAGG TGTTGGTCCT ACCGGTGTAA TAAGGACCCG 240 GCGCTAGGCA CGCCGTTAAA CCGAGCCCGT TATCCCCCTG GGCAAACGAC GCCCACGTAC 300 GGCCTACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC GTAGCCGGCG AGTTGACAAG 360 GGCCAGTGGG GGCCGGGCAA TGAGGGGAAG GACCCCTCTT GCTGCCCGAT CCGGGGGAGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTTTTCTTC CTATACCGAT CATGGCAGTC CTTCTGCTTC 540 TACTCGTGGT GGAGGCCGGG GCCATTCTGG CCCCGGCCAC ACATGCTTGT AGTGCTAAGG 600 GGCAATATTT CCTCACAAAC TGTTGCGCCC TG 632 (2) INFORMATION FOR SEQ ID NO: 66: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 66: CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GAYGCAATGG CCCCGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGT CCCTCTTGTG 180 CCTGTGGAGG GAAAGCGCAC GGTCCACAGG TGTTGGTCCT ACCGGTGTAA TAAGGACCCG 240 GCGCTAGGCA CGCCGTTAAA CCGAGTCCGT GAACCCCCTG GGCAAACGAC GCCCATGTAC 300 GGTCTACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC GTAGCCGGCG AGTTGGCAAA 360 GACCAGTGGG GGCCGGGCGA GAGGGGGAAG GACCCCCCTC GCTGCCCGTT CCGGGGGTGC 420 GGAAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTACTCTTC CTATACCAAT CATGGCAGTT CTTCTGCTTC 540 TACTCGTTGT GGAGGCCGGG GCCATTTTGG CCCCGGCCAC CCATGCTTGT AGTGCTGGGG 600 GGCAATATTT CCTCACAAAC TGTTGCGCCC TG 632 (2) INFORMATION FOR SEQ ID NO: 67: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 633 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67: CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTGAAGTA GACGCAATGG CCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AGGCTAAGGT CCCTCTTGTG 180 CTTATGGAAG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCCG 240 GCGATAGGCA CGCCGTTAAA CCGAGACCGT TGCCCCTCTG GGCAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGAAG GACCCCCACC GCTGCCCTTC CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTTTCTTTT CCTATACCGA TCATGGCAGT CCTTCTGCTC 540 TCACTCGTGG TGGAGGCCGG GGCCATTCTG GCCCCGGCCA CCCATGCTTG TAGTGCGAAG 600 GGGCAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2 ) INFORMATION FOR SEQ ID NO : 68 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 632 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear (ii) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 68 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG CCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATA GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AGGCTAAGGT CCCTCTTGTG 180 CTTATGGAAG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCCG 240 GCGATAGGCA CGCCGTTAAA CCGAGACCGT TGCCCCTCTG GGCAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC TTTGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGAAG GACCCCCACC GCTGCCCTTC CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTTCTCTTC CTATACCGAT CATGGCAGTC CTTCTGCTCT 540 TACTCGTGGT GGAGGCCGGG GCCATTCTGG CCCCGGCCAC CCATGCTTGT AGTGCGAAGG 600 GGCAATATTT CCTCACAAAC TGTTGCGCCC TG 632 ( 2 ) INFORMATION FOR SEQ ID NO : 69 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 69 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG TCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGC CCCTCTTGTG 180 CCTATGGAGG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCCG 240 GCGATAGGCA CGCCGTTAAA CCGAGACCGT TACCCCTCTG GGTAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTACAAT GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGATG GACCCCCACC GCTGCCCATT CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTTTCTTTC CTATACCGAT CATGGCAGTC CTTCTGCTCT 540 TACTCGTTGT GGAGGCCGGG GCCATTCTGG CCCCGGCCAC CCATGCTTGT GGTGCGCAGG 600 GACAATATTT CCTCACAAAC TGTTGCGCCC TG 632 ( 2 ) INFORMATION FOR SEQ ID NO : 70 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 633 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 70 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG TCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGC CCCTCTTGTG 180 CCTATGGAGG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCCG 240 GCGATAGGCA CGCCGTTAAA CCGAGACCGT TACCCCTCTG GGTAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTACAAT GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGATG GACCCCCACC GCTGCCCATT CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 CTCGGGTGAG GGCGGGTGGC ATTTTTCTTT CCTATACCGA TCATGGCAGT CCTTCTGCTC 540 TTACTCGTTG TGGAGGCCGG GGCCATTCTG GCCCCGGCCA CCCATGCTTG TGGTGCGCAG 600 GGACAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2) INFORMATION FOR SEQ ID NO:71: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 71 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG TCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGC CCCTCTTGTG 180 CCTATGGAGG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCCG 240 GCGATAGGCA CGCCGTTAAA CCGAGACCGT TACCCCTCTG GGTAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTACAAT GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGATG GACCCCCACC GCTGCTCATT CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ATTTTCTTTC CTATACCGAT CATGGCAGTC CTTCTGCTCT 540 TACTCGTTGT GGAGGCCGGG GCCATCCTGG CCCCGGCCAC CCATGCTTGT GGTGCGCAGG 600 GACAATATTT CCTCACAAAC TGTTGCGCCC TG 632 (2 ) INFORMATION FOR SEQ ID NO : 72 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 633 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear (ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 72 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG TCCAGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGC CCCTCTTGTG 180 CCTATGGAGG GAAAGCGCAC GGTCCACAGG TGTTGATCCT ACCGGTGTAA TAAGGATCTG 240 GCGATAGGCA CGCCGTTAAA CCGAGACCGT TACCCCTCTG GGTAAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTACAAT GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAG 360 GACCAGTGGG GGCCGGGCGG GAGGGGGATG GACCCCCACC GCTGCCCATT CCGAGGGGGC 420 GGGAAATGCA TGGGGCCACC ' CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAGCC 480 TTCGGGTGAG GGCGGGTGGC ACTTTTCTTT CCTATACCGA TCATGGCAGT CCTTCTGCTC 540 TTACTCGTTG TGGAGGCCGG GGCCATTCTG GCCCCGGCCA CCCATACTTG TGGTGCGCAG 600 GGACAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2 ) INFORMATION FOR SEQ ID NO : 73 : (i) SEQUENCE CHARACTERISTICS : (A) LENGTH : 630 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi) SEQUENCE DESCRIPTION : SEQ ID NO : 73 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCAAAATA GACGTGATGG CCCTGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGCCA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCT TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AGGCAACGGT CCCTCTCGCG 180 CTTACGGAGA GAAGGCGCAC GGTCCACAGG TGTTGGTCCT ACCGGTGTGA ATAAGGACTC 240 GGCGTTAGGC TCGCCGTTAA ACCGAGCCCG TTAACCCCCT GGGCAAACGA CGCCCACGTA 300 CGGTCTACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CAATGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGCCGGGTG GGGGGAAGGA CCCCCCTCAC TGCCCTTCCC GGGGGTGCGG 420 GAAATGCATG GGGCCACCCA GCTCCGCGGC GGCCTACAGC CGGGGTAGCC CAAGAACCTT 480 CGGGTGAGGG CGGGTGGCAT TTTTCTTCCT ATACCGATCA TGGCAGTCCT CCTGCTCTTC 540 TTCGTGGTGG AGGCCGGGGC CATTTTGGCC CCGGCCACCC ATGCTTGCAG TGCAAAGGGG 600 CAATACTTCC TCACAAACTG TTGCGCCCTG 630 (2) INFORMATION FOR SEQ ID NO:74: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 633 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:74: CACTGGGTGS AAGCCCCAGA AACCGACGCC TACTAAAGTA GACGCAATGG CCCCGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AAGCAAAGGT CCCTCTTGTG 180 CCTGTGGAGG GAAAGCGCAC GGTCCACAGG TGTTGGTCCT ACCGGTGTAA TAAGGACCCG 240 GCGTTAGGCA CGCCGTTAAA CCGAGCCCGT TATCTCCCTG GGCAAACGAC GCCCACGTAC 300 GGCCAACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC TTAGCCGGCG AGTTGACAAT 360 GGCCAGTGGG GGCCGGGCGA GAGGGGGAAG GACCCCCCTC GCTGCCCATT CCGGGGGAGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAACC 480 TTCGGGTGAG GGCGGGTGGC ATTTTTCTTT CCTATACCGA TCATGGCAGT CCTTCTGCTC 540 TTACTCGTTG TGGAGGCCGG GGCCATTTTG GCCCCGGCCA CCCATGCTTG TAGTGCTAAA 600 GGGCAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2) INFORMATION FOR SEQ ID NO:75: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 589 base pairs- (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:75: ACCGACGCCT ATCAAAGTAG ACGTGATAGC CCCGCGCCAA ACTGGCGACA GGCCAAAAGG 60 TGGTGGATGG GTGATGACAG GGTTGGTAGG TCGTAAATCC CGGTCATCCT GGTAGCCACT 120 ATAGGTGGGT CTTAAAGGGC GGCTACGGTC CCTCTCGCGC TTATGGAGAG AAAGCGCACG 180 GTCCACAGGT GTTGGcCCTA CCGGTGTAAT AAGGGCCCGG CGCTAGGCAC GCCGTTAAAC 240 CGAGACCGTT ACCCCCCTGG GCAAACGACG CCCACGTACG GCCCACGTCG CCCTTCAATG 300 TCTCTCTTGA CCAATAGGCT ATGCCGGCGA GTTGACAAGG GCCAGTGGGG GCCGGGCGGC 360 AGGGGAAGGA CCCCTGTCGC TGCCCTTCCC GGGGGGGCGG GAAATGCATG GGGCCACCCA 420 GCTCCGCGGC GCCCTACAGC CGGGGTAGTC CAAGAACCTT CGGGTGAGGA CGGGTGGCAT 480 TTCTTTTCCT ATACCGATCA TGGCAGTCTT TCTGCTCTTA CTCGTGGTGG' AGGCCGGGGC 540 CATTCTGGCC CCGGCCACAC ACGCTTGTAG TGCTAAAGGG CAATACTTC 589 (2) INFORMATION FOR SEQ ID NO:76: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 76 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCAAAGTA GACGTGATGG CCCCGCGCCA 60 AACTGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAAGGG CGGCTACGGT CCCTCTCGCG 180 CTTATGGAGA GAAAGCGCAC GGTCCACAGG TGTTGGCCCT ACCGGTGTAA TAAGGGCCCG 240 GCGCTAGGCA CGCCGTTAAA CCGAGACCGT TACCCCCCTG GGCAAACGAC GCCCACGTAC 300 GGCCCACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC TATGCCGGCG AGTTGACAAG 360 GGCCAGTGGG GGCCGGGCGG CAGGGGAAGG ACCCCTGTCG CTGCCCTTCC CGGGGGGGCG 420 GGAAATGCAT GGGGCCACCC AGCTCCGCGG CGCCCTACAG CCGGGGTAGT CCAAGAACCT 480 TCGGGTGAGG ACGGGTGGCA TTTTCTTTTC CTATACCGAT CATGGCCGTC CTTCTGCTCT 540 TACTCGTGGT GGAGGCCGGG GCCATTCTGG CCCCGGCCAC ACACGCTTGT GGTGCTAAAG 600 GGCAATACTT CCTCACAAAC TGTTGCGCCC TG 632 (2) INFORMATION FOR SEQ ID NO:77: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 632 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:77: CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCAAAATA GACGTGATGG CCCTGCGCCG 60 AACCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGCCA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCT TGGTAGCCAC TATAGGTGGG TCTTAAGGGG AGGCAACGGT CCCTCTCGCG 180 CTTACGGAGA GAAGGCGCAC GGTCCACAGG TGTTGGTCCT ACCGGTGTGA ATAAGGACTC 240 GGCGTTAGGC TCGCCGTTAA ACCGAGCCCG TTAACCCCCT GGGCAAACGA CGCCCACGTA 300 CGGTCTACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CAATGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGCCGGGTG GGGGGGGAAG GACCCCCCTC ACTGCCCTTC CCGGGGGTGC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTACA GCCGGGGTAG CCCAAGAACC 480 TTCGGGTGAG GGCGGGTGGC ATTTTTCTTC CTATACCGAT CATGGCAGTC CTCCTGCTCT 540 TCTTCGTGGT GGAGGCCGGG GCCATTTTGG CCCCGGCCAC CCATGCTTGC AGTGCAAAGG 600 GGCAATACTT CCTCACAAAC TGTTGCGCCC TG 632 (2) INFORMATION FOR SEQ ID NO:78: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 436 base pairε (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:78: GGTGCAAGCC CCAGAAACCG ACGCCTATTT AAACAGACGT TATGAACCGG CGCCGACCCG 60 GCGACCGGCC AAAAGGTGGT GGATGGGTGA TGCCAGGGTT GGTAGGTCGT AAATCCCGGT 120 CATCTTGGTA GCCACTATAG GTGGGTCTTA AGGGTTGGTC AAGGTCCCTC TAGCGCTTGT 180 GGCGAGAAAG CGCACGGTCC ACAGGTGTTG GCCCTACCGG TGTGAATAAG GGCCCGACGT 240 CAGGCTCGTC GTTAAACCGA GCCCATTACC CACCTGGGCA AACAACGCCC ACGTACGGTC 300 CACGTCGCCC TACAATGTCT CTCTTGACCA ATAGGCTTTG CCGGCGAGTT GACAAGGACC 360 AGTGGGGGCT GGGCGGCAGG GGAAGGACCC CTGTCGCTGC CCTTCCCGGT GGGGTGGGGA 420 ATGCATGGGG CCACCC 436 (2) INFORMATION FOR SEQ ID NO:79: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 349 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:79: AGGGTTGGTA GGTCGTAAAT CCCGGTCACC TTGGTAGCCA CTATAGGTGG GTCTTAAGAG 60 AAGGTTAAGA TTCCTCTTGT GCCTGCGGCG AGACCGCGCA CGGTCCACAG GTGTTGGCCC 120 TACCGGTGGG AATAAGGGCC CGACGTCAGG CTCGTCGTTA AACCGAGCCC GTCACCCACC 180 TGGGCAAACG ACGCCCACGT ATGGTCCACG TCGCCCTTCA ATGCCTCTCT TGGCCAATAG 240 GTTTATCCGG CGAGTTGACA AGGACCAGTG GGGGCCGGGG GTTCGGGGAA GGACCTCGTA 300 CCCTGCCCTT CCCGGTGGAA CGGGAAATGC ATGGGGCCAC CCAGCTCCG 349 (2) INFORMATION FOR SEQ ID NO:80: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 355 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:80: AGGGTTGGTA GGTCGTAAAT CCCGGTCATC TTGGTAGCCA CTATAGGTGG GTCTTAAGGG 60 GAGGTAAAGG TCCCTCTTGC GCTTATGGAG GAACAGCGCA CGGTCCACAG GTGTTGGTCC 120 TACCGGTGTA ATAAGGACCC GGCGCTAGGC ACGCCGTTAA ACCGAGCCCG- TTACCCTCCT 180 GGGCAAACGA CGCCCACGTA CGGTCCACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG 240 TTCTACCGGC GAGTTGACAA GGACCAGTGG GGGCCGGGCG GGAGGGGGAA GGACCCCCAC 300 CGTCGCCCTT CCCGGAGGGG CGGGAAATGC ATGGGGCCAC CCAGCTCCGG CATGG 355 ( 2 ) INFORMATION FOR SEQ ID NO : 81 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 636 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 81 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCTAAGTA GACGCAATGA CTCGGCGCCG 60 ACTCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGCCA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCT TGGTAGCCAC TATAGGTGGG TCTTAAGAGA AGGTTAAGAT TCCTCTTGTG 180 CCTGCGGCGA GACCGCGCAC GGTCCACAGG TGTTGGCCCT ACCGGTGTAA TAAGGGCCCG 240 ACGTCAGGCT CGTCGTTAAA CCGAGCCCGT CACCCACCTG GGCGAACGAC GCCCACGTAC 300 GGTCCACGTC GCCCTTCAAT GCCTCTCTTG GCCAATAGGA GATTCCTCCG GCGAGTTGGC 360 AAGGACCAGT GGGGGCCGGG GGTCACAGGG AAGGACCCTG GACCCTGCCC ATCCCGGTGG 420 GCCGGGAAAT GCATGGGGCC ACCCAGCTCC GCGGCGGCCT GCAGCCGGGG TAGCCCAAGA 480 ATCCTTCGGG TGAGGGCGGG TGGCATTTCT CTTTCCTATA CCATCATGGC AGTCCTTCTG 540 CTCCTTCTCG TGGTTGAGGC CGGGGCCATT CTGGCCCCGG CCACCCACGC TTGTAGAGCG 600 AATGGGCAAT ATTTCCTCAC AAACTGTTGC GCCCTG 636 ( 2 ) INFORMATION FOR SEQ ID NO : 82 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 633 base pairs - (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear (ii) MOLECULE TYPE: DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 82 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCTAAGTA GACGCAATGA CTCGGCGCCG 60 ACCCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGCCA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCT TGGTAGCCAC TATAGGTGGG TCTTAAGGGA AGGTTAAGAT TCCTCTTGTG 180 CCTGTGGCGA GACAGCGCAC GGTCCACAGG TGTTGGCCCT ACCGGTGTGA ATAAGGGCCC 240 GACGTCAGGC TCGTCGTTAA ACCGAGACCG ACACCCACCT GGGCAAACGA CGCCCACGTA 300 CGGTCCACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CTTTGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGCCGGGGG CTGGGAGAAG GACTCCCACG CCCCGCCCTT CCCGGGGGGA 420 CGGGAAATGC ATGGGGCCAC CCAGCTCCGC GGCGGCCTGC AGCCGGGGTA GTCCAAGAGC 480 CTTCGGGTGA GGACGGGTGG CATTTCTTTT TCTACACCGA TCATGGCAGT CCTTCTGCTC 540 TTCTTCGTGG TTGAGGCCGG GGCCATTCTG GCCCCGGCCA CCCACGCTTG TCGAGCTGGT 600 GGGCAATATT TCCTCACAAA CTGTTGCGCC CTG 633 ( 2 ) INFORMATION FOR SEQ ID NO : 83 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH : 631 base pairs (B) TYPE : nucleic acid (C) STRANDEDNESS : double (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 83 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCAAAATA GACGTGATGG CCCCGCGCCG 60 AACTGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCATCC TGGTAGCCAC TATAGGTGGG TCTTAAAGGG AGGCTACGGT CCCTCTCGCG 180 CTTACGGAGA GAAAGCGCAC GGTTCACAGG TGTTGGCCCT ACCGGTGTAA TAAGGGCCCG 240 GCGCTAGGCA CGCCGTTAAA CCGAGACCGT TACCCTCCTG GGCAAACGAC GCCCACGTAC 300 GGCCCACGTC GCCCTTCAAT GTCTCTCTTG ACCAATAGGC AATGCCGGCG AGTTGACAAG 360 GGCCAGTGGG GGCCGGCGGA CAGGGGAAGG ACCCCTGTCG CTGCCCTTCC CGGAGGGACG 420 GGAAATGCAT GGGGCCACCC AGCTCCGCGG CGGCCTACAG CCGGGGTAGC CCAAGAACCT 480 TCGGGTGAGG GCGGGTGGCA TTTCTTTTCC TATACCAATC ATGGCAGTCC TTCTGCTCTT 540 ACTCGTTGTG GAGGCCGGGG CCATYCTGGC CCCGGCCACC CATGCTTGTA GCGCTAAAGG 600 GCAATATTTC CTCACAAACT GTTGCGCCCT G 631 (2) INFORMATION FOR SEQ ID NO:84: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 633 base pairε (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:84: CACTGGGTGY RARCCCCAGA AACCGACGCC TATCTAAGTA GACGCAATGA CTCGGCGCCG 60 ACTCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCACCT TGGTAGCCAC TATAGGTGGG TCTTAAGAGA AGGTTAAGAT TCCTCTTGTG 180 CCTGCGGCGA GACCGCGCAC GGTCCACAGG TGCTGGCCTT ACCGGTGCAA ATAAAGGCCC 240 GACGTCAGGC TCGTCGTTAA ACCGAGCCCG TTACCCACCT GGGCAAACGA CGCCCACGTA 300 CGGTCCACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CTTAGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGCCGGGGG TTTGGGGAAG GACCCCAAGC CCTGCCCTTC CCGGTGGGCC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CGCAAGAATC 480 CTTCGGGTGA GGGCGGGTGG CATTTTTCTT TTCTATACCA TCATGGCAGT CCTTCTGCTC 540 CTTCTCGTGG TTGAGGCCGG GGCCATCCTG GCCCCGGCCA CCCACGCGTG TCGAGCGAAT 600 GGGCAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2) INFORMATION FOR SEQ ID NO:85: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 634 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:85: CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCTAAGTA GACGCAATGA CTCGGCGCCA 60 ACTCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCACCT TGGTAGCCAC TATAGGTGGG TCTTAAGAGA AGGTTAAGAT TCCTCTTGTG 180 CCTGCGGCGA GACCGCGCAC GGTCCACAGG TGTTGGCCCT ACCGGTGTGA ATAAGGGCCC 240 GACGTCAGGC TCGTCGTTAA ACCGAGCCCG TCACCCACCT GGGCAAACGT CGCCCACGTA 300 CGGTCCACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CTTAGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGTCGGGGG CTTGGGGAGG GACCCCAAGT CCTGCCCTTC CCGGTGGGCC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAATC 480 CTTCGGGTGA GGGCGGGTGG CATTTTCTCT TTTCTATACC ATCATGGCAG TCCTTCTGCT 540 CCTTCTCGTG GTCGAGGCCG GGGCCATTCT GGCCCCGGCC ACCCACGCTT GTCGAGCGAA 600 TGGGCAATAC TTCCTCACAA ACTGTTGCGC CCTG 634 ( 2 ) INFORMATION FOR SEQ ID NO : 86 : (i) SEQUENCE CHARACTERISTICS : (A) LENGTH : 633 base pairs • (B) TYPE : nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii ) MOLECULE TYPE : DNA (genomic) (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 86 : CACTGGGTGC AAGCCCCAGA AACCGACGCC TATCTAAGTA GACGCGATGA CTCGGCGCCG 60 ACTCGGCGAC CGGCCAAAAG GTGGTGGATG GGTGATGACA GGGTTGGTAG GTCGTAAATC 120 CCGGTCACCT TGGTAGCCAC TATAGGTGGG TCTTAAGAGA AGGTTAAGAT TCCTCTCGTG 180 CCTGCGGCGA GACCGCGCAC GGTCCGCAGG TGTAGGCCCT ACCGGTGTGA ATAAGGGCCC 240 GACGTCAGGC TCGTCGTTAA ACCGAGCCCG TCACCCACCT GGGCTAACGA CGCCCACGTA 300 CGGTCCACGT CGCCCTTCAA TGTCTCTCTT GACCAATAGG CTTAGCCGGC GAGTTGACAA 360 GGACCAGTGG GGGCCGGGGG CTTGGAGAAG GACTCCAAGT CCTGCCCTTC CCGGTGGGCC 420 GGGAAATGCA TGGGGCCACC CAGCTCCGCG GCGGCCTGCA GCCGGGGTAG CCCAAGAACC 480 CTTCGGGTGA GGGCGGGTGG CATTTCTTTT TCCTATACCA TCATGGCAGT CCTTCTGCTC 540 TTTCTCGTGG TTGAGGCCGG GGCCATTTTG GCCCCGGCCA CCCACGCTTG TCGAGCGAAT 600 GGGCAATATT TCCTCACAAA CTGTTGCGCC CTG 633 (2) INFORMATION FOR SEQ ID NO:87: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:87: AGCAGAAGGA CTGCCATGAT 20 (2) INFORMATION FOR SEQ ID NO:88: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:88: GGNRMKRTYC CYTTTTATGG GCATGG 26 (2) INFORMATION FOR SEQ ID NO:89: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 89: ACNACNAGGT CNCCRTCYTT GATGAT 26

Claims: CLAIMS 1. An oligonucleotide identified as SEQUENCE ID NO 51 and complements thereof. 2. An oligonucleotide identified as SEQUENCE ID NO 53 and complements thereof. 3. An oligonucleotide identified as SEQUENCE ID NO 54 and complements thereof. 4. An oligonucleotide identified as SEQUENCE ID NO 55 and complements thereof. 5. An oligonucleotide identified as SEQUENCE ID NO 56 and complements thereof. 6. An oligonucleotide identified as SEQUENCE ID NO 57 and complements thereof. 7. An oligonucleotide identified as SEQUENCE ID NO 87 and complements thereof. 8. A method of detecting target HGBV-C nucleotides in a test sample, comprising: a. contacting a target HGBV nucleotide with at least one HGBV-C specific oligonucleotide or complement thereof; and b . detecting the presence of the target in the test sample. 9. The method of claim 8 wherein said oligonucleltide is selected from the group consisting of SEQUENCE ID NO 51, SEQUENCE ID NO 53, SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 56, SEQUENCE ID NO 57 and SEQUENCE ID NO 87 and complements thereof. 10. The method of claim 8 wherein said oligonucleotide is selected from the group consisting of SEQUENCE ID NO 2, SEQUENCE ID NO 3, SEQUENCE ID NO 4, SEQUENCE ID NO 5, SEQUENCE ID NO 6, SEQUENCE ID NO 9, SEQUENCE ID NO 10, SEQUENCE ID NO 11, SEQUENCE ID NO 13, SEQUENCE ID NO 13, SEQUENCE ID NO 14, SEQUENCE ID NO 15, SEQUENCE ID NO 16, SEQUENCE ID NO 17, SEQUENCE ID NO 18, SEQUENCE ID NO 19, SEQUENCE ID NO 20, SEQUENCE ID NO 21 , SEQUENCE ID NO 22, SEQUENCE ID NO 23, SEQUENCE ID NO 24, SEQUENCE ID NO 60, SEQUENCE ID NO 61, SEQUENCE ID NO 62, SEQUENCE ID NO 63, SEQUENCE ID NO 64, SEQUENCE ID-NO 65, SEQUENCE ID NO 66, SEQUENCE ID NO 67, SEQUENCE ID NO 68, SEQUENCE ID NO 69, SEQUENCE ID NO 70, SEQUENCE ID NO 71, SEQUENCE ID NO 72, SEQUENCE ID NO 73, SEQUENCE ID NO 74, SEQUENCE ID NO 75, SEQUENCE ID NO 76, SEQUENCE ID NO 77, SEQUENCE ID NO 78, SEQUENCE ID NO 79, SEQUENCE ID NO 80, SEQUENCE ID NO 81 , SEQUENCE ID NO 82, SEQUENCE ID NO 83, SEQUENCE ID NO 84, SEQUENCE ID NO 85 and SEQUENCE ID NO 86 and complements thereof. 11. A method of detecting target HGBV-C nucleotides in a test sample, comprising: a. contacting a target HGBV nucleotide with at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 51 , SEQUENCE ID NO 53, SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 56, SEQUENCE ID NO 57 and SEQUENCE ID NO 87 and complements thereof; and b . detecting the presence of the target in the test sample. 12. The method of claim 8 wherein said target HGBV-C nucleotide is attached to a solid phase prior to performing step (a). 13. A method for amplifying 5' end cDNA of hepatitis GB virus in a test sample, said method comprising: a. performing reverse transcription with random primers; b . amplifying the cDNA obtained from step (a) by using other oligonucleotide primer(s) of HGBV-C as sense and antisense primer(s) in a first- stage PCR to obtain amplified cDNA; c . detecting the presence of the HGBV-C amplicon in the test sample. 14. The method of claim 13 wherein said amplification of step (b) is performed by the polymerase chain reaction. 15. The method of claim 13 wherein said test sample is attached to a solid phase prior to performing step (a). 16. The method of claim 13, wherein said detection step comprises utilizing a detectable label capable of generating a measurable signal. 17. The method of claim 16, wherein said detectable label is attached to a solid phase. 18. A method for amplifying 5' end cDNA of hepatitis GB virus in a test sample, said method comprising: a. performing reverse transcription with random primers; b . amplifying the cDNA obtained from step (a) by using other oligonucleotide primer(s) as sense and antisense primer(s) in a first-stage PCR to obtain amplified cDNA wherein said sense primer is selected from the group consisting of SEQUENCE ID NO 51, 53, 56 and the antisense primer are selected from the group consisting of 54, 55, 57 87; c . detecting the presence of the amplicon in the test sample. 19. The method of claim 18 wherein said amplification of step (b) is performed by the polymerase chain reaction. 20. The method of claim 18 wherein said test sample is attached to a solid phase prior to step (a). 21. The method of claim 18, wherein said detection step comprises utilizing a detectable label capable of generating a measurable signal. 22. The method of claim 18, wherein said detectable label is attached to a solid phase. 23. A method of detecting HGBV-C in a test sample suspected of containing target HGBV, comprising: a. contacting said test sample with at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 51, SEQUENCE ID NO 56 and SEQUENCE ID NO 53 as a sense primer and with at least one oligonucleotide selected from the group consisting of SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 57 and SEQUENCE ID NO 87 as an anti-sense primer and amplifying same to obtain a first stage reaction product; b . contacting said first stage reaction product with at least one of said oiigonucleotides of step (a) and a second oligonucleotide selected from the group consisting of SEQUENCE ID NO 51, SEQUENCE ID NO 53, SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 56, SEQUENCE ID NO 57 and SEQUENCE ID NO 87, with the proviso that the second oligonucleotide is located 3' to the first oligonucleotide utilized and is of opposite sense to said first oligonucleotide; and c. detecting said HGBV target. 24. The method of claim 23, wherein said amplification is performed by the polymerase chain reaction. 25. The method of claim 23, wherein said test sample is attached to a solid phase prior to performing step (a). 26. The method of claim 23, wherein said detection step comprises utilizing a detectable label capable of generating a measurable signal. 27. The method of claim 26, wherein said detectable label is attached to a solid phase. 28. Primers useful for GAP Ligase Chain Reaction selected from the group consisting of SEQUENCE ED NO 35, SEQUENCE ID NO 36, SEQUENCE ID NO 37, SEQUENCE ID NO 38, SEQUENCE ID NO 39, SEQUENCE ID NO 40, SEQUENCE ID NO 41, SEQUENC ID NO 43, SEQUENCE ID NO 44, SEQUENCE ID NO 45, SEQUENCE ID NO 46, SEQUENCE ID NO 47, SEQUENCE ID NO 48, SEQUENCE ID NO 49 and SEQUENCE ID NO 50, and complements thereof. 29. A test kit useful for detecting HGBV-C target in a test sample, comprising a container containing at least one oligonucleotide selected from the group consisting of SEQUENCE ED NO 51- SEQUENCE ID NO 53, SEQUENCE ID NO 54, SEQUENCE ID NO 55, SEQUENCE ID NO 56, SEQUENCE ID NO 57 and SEQUENCE ID NO 87 and complements thereof.

Inventors: BIRKENMEYER LARRY, CHALMERS MICHELLE, DAWSON GEORGE J, DESAI SURESH M, ERKER JAMES C, LEARY THOMAS P, MUERHOFF ANTHONY S, MUSHAHWAR ISA K, SIMONS JOHN N, BIRKENMEYER, LARRY, CHALMERS, MICHELLE, DAWSON, GEORGE, J., DESAI, SURESH, M., ERKER, JAMES, C., LEARY, THOMAS, P., MUERHOFF, ANTHONY, S., MUSHAHWAR, ISA, K., SIMONS, JOHN, N.
Applicants: ABBOTT LAB, ABBOTT LABORATORIES
Application year: 1996
Main classification: G01N
IPC codes: C07K14, C12N15, C12Q1, G01N33