N alpha -2-(4-NITROPHENYLSULFONYL)ETHOXYCARBONYL-AMINO ACIDS

Abstract: Protected amino acid derivatives of general formula (I) wherein R1 represent hydrogen atom, and R2 may represent hydroxymethyl, 1-hydroxyethyl, 4-hydroxybenzyl, imidazolyl-2-methyl, benzyloxymethyl, 1-benzyloxyethyl, 4-benzyloxybenzyl, benzyloxycarbonylmethyl, 2-(benzyloxycarbonyl)ethyl, S-benzylthiomethyl, S-(diphenylmethyl)thiomethyl, 4-(benzyloxycarbamido)butyl, 3-guanidinopropyl, 3-(N<G>-toluenesulfonyl)guanidinopropyl, 3-(N<G>-nitro)guanidinopropyl, 3-[N<G>-(4-methoxy-2,3,6-trimethylbenzenesulfonyl)]-guanidinopropyl, N-(4,4'-dimethoxybenzhydryl)carboxamidomethyl, 2-[N-(4,4'-dimethoxybenzhydryl)carboxamido]ethyl, S-tert-butyldithiomethyl, 4-[2-(4-nitrophenylsulfonyl)ethoxycarbamido]-butyl, propyl, butyl, ethyl, 3-(benzyloxycarbamido)propyl, or 3-(tert-butoxycarbamido)propyl. Methods for the preparation of said derivatives are provided.

Description: N α -2- (4-Nitrophenylsulfonyl) ethoxycarbonyl-amino acids Background of the invention The field of the invention concerns new protected amino acid derivatives useful for peptide synthesis, namely, N α -2-(4- nitrophenylsulfonyl) ethoxycarbonyl-amino acids having the general formula I : I wherein R x represents hydrogen atom, and R 2 may represent hydroxymethyl, 1-hydroxyethyl, 4-hydroxybenzyl, imidazolyl-2- methyl, benzyloxymethyl, 1-benzyloxyethyl, 4-benzyloxybenzyl, benzyϊoxycarbonylmethyl, 2- (benzyloxycarbonyl) ethyl, S- benzylthiomethyl, S- (diphenylmethyl ) thiomethyl, 4- (benzyloxycarbamido) butyl, 3-guanidinopropyl, 3- (N G - toluenesulfonyl) guanidinopropyl, 3- (N G -nitro) guanidinopropyl, 3- [N G - (4-methoxy-2, 3, β-trimethylbenzenesulfonyl) ] - guanidinopropyl , N- ( 4 , 4 ' -dimethoxybenzhydryl ) carboxamidomethyl , 2- [N- (4,4' -dimethoxybenzhydryl) carboxa ido] ethyl, S-tert- butyldithiomethyl, 4- [2- (4-nitrophenylsulfonyl) ethoxycarbamido] -butyl, propyl, butyl, ethyl, 3- (benzyloxycarbamido) propyl, or 3- (tert-butoxycarbamido) propyl, and methods for the preparation thereof. N„-2- (4-Nitrophenylsulfonyl) ethoxycarbonyl-amino acids (Nsc-amino acids) I represent a class of protected amino acid derivatives which are used in the chemical synthesis of peptides. In these derivatives, N α -2- (4-nitrophenylsulfonyl) -ethoxycarbonyl (Nsc) group serves as a temporary N α -protection which can be selectively removed after each step of the peptide chain elongation. Nsc-Group is fairly resistant to acidic reagents and is cleaved according to the β-elimination mechanism by organic bases in aprotic solvents. Mild conditions of the cleavage allow to use the temporary N α -Nsc- protection in the peptide synthesis together with the acid- sensitive side chain protection of widely used t-butyl or benzyl type, thus providing the so-called "orthogonality" of the synthetic strategy. Recently N α -Nsc-amino acids, methods for their preparaton, and their employment for the solid phase peptids synthesis have been disclosed in International Publication No. W096/25394. However, only minimal set of N α -Nsc-derivatives of proteogenic amino acids has been described, which is intended for the solid phase peptide synthesis with full side-chain protection of acid-labile tert-butyl or compatible type. Summary of the Invention It is, therefore, desirable to develop new N α -Nsc-amino acids to extend the list of derivatives available for solid phase synthesis. On the other hand, it is useful to provide N α -Nsc-amino acid derivatives with another type of side protection (e.g. , benzyl of compatible) or without side protection which may give new opportunities for liquid phase peptide synthesis. An object of the present invention is to provide new N α - Nsc-a ino acids derivatives, more particularly, N α -2- (nitrophenylsulfonyl) ethoxycarbonyl-amino acids having the general formula I : wherein R λ represents hydrogen atom, and R 2 may represent hydroxymethyl, 1-hydroxyethyl, 4-hydroxybenzyl, imidazolyl-2- methyl, benzyloxymethyl, 1-benzyloxyethyl, 4-benzyloxybenzyl, benzyloxycarbonylmethyl, 2- (benzyloxycarbonyl) ethyl, S- benzylthiomethyl, S- (diphenylmethyl) thiomethyl, 4- (benzyloxycarbamido) butyl, 3-guanidinopropyl, 3- (N G - toluenesulfonyl) guanidinopropyl, 3- (N G -nitro) guanidinopropyl, 3- [N G - (4-methoxy-2, 3, 6-trimethylbenzenesulfonyl) ] - guanidinopropyl, N- (4, 4 'dimethoxybenzhydryl) carboxamidomethyl, 2- [N- (4, 4 '-dimethoxybenzhydryl) carboxamido] ethyl, S-tert- butyldithiomethyl, 4- [2- (4-nitrophenylsulfonyl) ethoxycarbamido] -butyl, propyl, butyl, ethyl, 3- (benzyloxycarbamido) propyl, or 3- (tert-butoxycarbamido) propyl, which can be employed as N α - protected amino acid derivatives in peptide synthesis. Another object of the present invention is to provide methods for the preparation of said N α -Nsc-amino acids. These and other objects of the present invention will be apparent from the following description Detailed Description of the Invention N α -Nsc-amino acids of the present invention (I) can be prepared by the treatment of amino acids of the general formula π, wherein R x and R 2 represent meanings given for formula I, with 2- (4-nitrophenylsulfonyl) ethylchloroformate πi in mixed aqueous/organic solvent in the presence of base and at the temperature from 0 to 40°C, preferably from 0 to 20°C (Scheme 1) . Scheme 1 Chloroformate HI is introduced into the reaction in amounts from 0.5 to 1.5 molar equivalents, preferably from 0.7 to 0.9, as related to amino acid. As an organic component of the solvent any aprotic organic solvent may be used which is capable to dissolve the acylating reagent and is mixible with water, for example, acetonitrile, DMF, tetrahydrofuran or dioxane. A base may be organic or inorganic base, for example, sodium or potassium carbonate, magnesium or calcium oxide, triethylamine, N-methylmorpholine . According to another method of the present invention, amino acids of the general formula II are firstly converted into N,0-trimethylsilyl dervatives using methods known in the art and then treated with equivalent amount of chloroformate HI in anhydrous organic solvent, for example, dichloromethane in the presence of base, for example, tertiary amine. After aqueous hydrolysis of intermediate acylated trimethylsilyl derivatives desirable N α -Nsc-amino acids I are obtained in a free from. Derivatives of the formula I, wherein R x is hydrogen, and R 2 represents N- (4, 4 '-dimethoxybenzhydryl) carboxamidomethyl or 2-[N-(4, 4 '-dimethoxybenzhydryl) carboxamido] ethyl, may be prepared by the reaction of the known derivatives I, wherein R x is hydrogen, and R 2 represents carboxamidomethyl or 2- (carboxamido) ethyl, with 4, 4 ' -dimethoxybenzhydrol in organic solvent in the presence of strong acid. As a solvent acetic acid may be used, and as a strong acid-sulfuric or methanesulfonic acid. Derivative of the formula I, wherein R x is hydrogen, and R 2 represents 3-guanidinopropyl (N α -Nsc-Arg-OH) , may be prepared by the reaction of arginine with 2- (4-nitrophenylthio) - ethylchloroformate in water in the presence of sodium bicarbonate, resulting N α -2- (4-nitrophenylthio) ethoxycarbonyl- arginine is then oxidized into desired N α -Nsc-arginine with hydrogen peroxide in organic solvent, for example, ethanol or acetic acid. Derivative of the formula I, wherein R x is hydrogen, and R 2 represents imidazolyl-2-methyl (N α -Nsc-His-OH) , may be prepared from N α -Nsc-N ιm -protected histidine derivative by selective deprotection of imidazole rign, for example, by selective acidic detritylation of known N α -Nsc-N im - triphenylmethyl-histidine . It is seen from molecular formula that compounds I have an asymmetric -carbon atom. Because α-carbon atom does not participate in reactions employed for the preparation of compounds I, so the configuration of this chiral center existing in starting amino acids II is retained in resulting N α -Nsc-derivatives I. Therefore it is obvious that the methods of the present invention can be used for the preparation of N α - Nsc-amino acids I in any chiral form(L or D) , as well as racemic compounds, depending on the configuration of the compound II. Meanings of R λ and R 2 substituents in derivatives of the formula I according to the present invention correspond to structures of side chains of known α-amino acids containing or not containing protective groups known in the art (Table 1). Table 1 Meanings of R x and 2 substituents in compounds I No Rl R2 Amino acid Abbreviation 1-1 H Hydroxymethyl Serine Nsc-Ser-OH 1-2 H 1-Hydroxyethyl Threonie Nsc-Thr-OH 1-3 H 4-Hydroxybenzyl Tyrosine Nsc-Tyr-OH 1-4 H Imidazolyl-2-methyl Histidine Nsc-His-OH 1-5 H Benzyloxymethyl O-Benzyl-serine Nsc-Ser(Bzl)-OH 1-6 H 1-Benzyloxyethyl O-Benzyl-theronine Nsc-Thr(Bzl)-OH 1-7 H 4-Benzyloxybenzyl O-Benzyl-tyrosine Nsc-Tyr(Bzl)-OH 1-8 H Benzyloxycarbonylmethyl Aspartic acid β -benzyl Nsc-Asp(OBzl)-OH -ester 1-9 H 2-(Benzyloxycarbonyl)ethyl Glutamic acid γ-benzyl Nsc-Glu(OBzl)-OH -ester I-10 H S-Benzylthiomethyl S-Benzyl-cysteine Nsc-Cys(Bzl)-OH I-11 H S-(Diphenylmethyl)thio S-Diphenylmethyl-cysteine Nsc-Cys(Dpm)-OH -methyl o Rl R2 Amino acid Abbreviation 1-12 H 4-(Benzyloxycarbamido) Nε-Benzyloxycarbonyl Nsc-Lys(Z)-OH -butyl -lysine 1-13 H 3 -Guanidinopropyl Arginine Nsc-Arg-OH 1-14 H 3-(N°-Toluenesulfonyl) N°-Toluenesulfonyl Nsc-Arg(Tos)-OH guanidinopropyl -arginine 1-15 H 3-(N°-Nitro)guanidino N°-Nitro-arginine Nsc-Arg(NO 2 )-OH -propyl 1-16 H 3-[N°-(4-Methoxy-2,3,6- N°-(4-Methoxy-2,3,6-tri Nsc-Arg(Mtr)-OH trimethyl-benzenesulfonyl)] -methylbenzenesulfonyl) guandinopropyl -arginine 1-17 H N-(4,4'-Dimethoxybenzhy Nβ-(4,4'-Dimethoxybenzhy Nsc-Asn(Mbh)-OH -dryl)carboxamidomethyl -dryl)-asρaragine 1-18 H 2-[n-(4,4'-Dimethoxybenzhy Nγ-(4,4'-Dimethoxybenzhy Nsc-Gln(Mbh)-OH -dryl)carboxamido]ethyl -dryl)-glutamine 1-19 H S-tert-Butyldithiomethyl S-tert-Butylthio-cysteine Nsc-Cys(StBu)-OH No Rl R2 Amino acid Abbreviation 1-20 H 4-[2-(4-Nitrophenylsulfo- Nε-[2-(4-Nitrophenylsulfon- Nsc-Lys(Nsc)-OH nyl)ethoxycarbamido]butyl yl)ethoxy carbonyl]-lysine 1-21 H Propyl Norvaline Nsc-Nva-OH 1-22 H Butyl Norleucine Nsc-Nle-OH 1-23 H Ethyl α-Aminobutyric acid Nsc-Abu-OH 1-24 H 3 -(benzyl oxycarbamido) Nδ-Benzyloxycarbonyl Nsc-Om(Z)-OH -propyl -omitine 1-25 H 3-(tert-Butoxycarbamido) Nδ-tert-Butoxycar- Nsc-Om(Boc)-OH -propyl bonyl-omitine Together with N α -Nsc-amino acids disclosed in International Publication No. W096/25394, the compounds of the formula I shown in Table 1 may be used to implement a wide variety of tactical approaches to the peptide synthesis, including not only solid phase but also solution syntheses. Using N α -Nsc-protection it is possible now to develop synthetic schemes of peptide assembly in solution on the basis of tert- butyl type or benzyl type or mixed type of side-chain protection, and also to apply minimal side-chain protection tactics. The present invention, therefore, is regarded as an addition to, and a continuation of, aforementioned International Publication No. 096/25394. The invention will now be described by way of examples which are provided as illustration and are not intended as being limiting. All of the amino acids in the following description have L-configuration unless otherwise indicated. Example 1 N α -Nsc-0-Benzyl-Tyrosine (1-7) 8.15 g of O-benzyl-tyrosine and 7.7 g of potassium carbonate were dissolved in 150 ml of water-dioxane mixture (3:1, v/v) and cooled in ice bath, then solution of 7.5 g of 2- (4-nitrophenylsulfonyl) ethyl chloroformate III in 70 ml of dioxane was added dropwise within 15 min with stirring. Cooling bath was removed and mixture was stirred for additional 20 min, then evaporated to ca. 100 ml under reduced pressure and transferred into separating funnel. 100 ml of water was added, and the resulting solution was extracted with 2 x 50 ml of ethyl acetate. Aqueous layer was separated, acidified to pH 2 with 40% sulfuric acid with cooling in ice bath, them extracted with 3 x 80 ml of ethyl acetate. Combined extracts were dried over anhydrous sodium sulfate and evaporated at reduce ' d pressure. Recrystallization of the residue from ethyl acetate-hexane gave the desired product 1-7 in a form of white crystalline powder(70%). For characterization see Table 2 (Example 7) . Example 2 N -Nsc-Threonine (1-2) . 3.58 g of threonine and 100 ml anhydrous dichloromethane were placed into 250 ml round-bottom flask equipped with reflux condenser and dropping funnel. To the suspension 9.7 ml of chlorotrimethylsilane was added with vigorous stirring, and the mixture was heated to boiling for 1 hr. The resulting solution was cooled in ice bath, then 9.0 ml of triethylamine and 9.0 g of chlorofor ate HI were added with stirring. The mixtire was stirred for 20 min in ice bath, then for additional 1.5 hr at room temperature. The solvent was evaporated at reduced pressure, and the residue was distributed between 200 ml of ethyl acetate and 250 ml of 2.5% aqueous sodium bicarbonate. Aqueous layer was separated, washed with 50 ml of ether, acidified to pH with 1 N hydrochloric acid, them extracted with 3 x 70 ml of ethyl acetate. Combined extracts were dried with anhydrous sodium sulfate and evaporated at reduced pressure. Trituration of the residue with petroleum ether gave the desired product 1-2 in a form of white powder (75%). For characterization see Table 2 (Example 7). Example 3 N α -Nsc-N δ -Benzyloxycarbonyl-Ornitine (1-24) . 8.0 g of N δ -benzyloxycarbonyl-ornitine and 120 ml of anhydrous dichloromethane were placed into 250 ml round-bottom flask equipped with reflux condenser and dropping funnel. To the mixture 12.7 ml of triethylamine and then 7 ml of chlorotrimethylsilane were added with vigorous stirring, and the mixtire was heated to boiling for 1.5 hr. The reaction mixture was then cooled in ice bath, 9.0 g of chloroformate HI was added at once, and stirring was continued for 1.5 hr at room temperature. The solvent was evaporated at reduced pressure, and the residue was distributed between 250 ml of ethyl acetate and 250 ml of 2.5% aqueous sodium bicarbonate. Aqueous layer was separated, washed with 50 ml of ether, acidified to pH 2 with 1 N hydrochloric acid, then extracted with 2 x 100 ml of ethyl acetate. Combined extracts were dried with anhydrous sodium sulfate and evaporated at reduced pressure. Recrystallization of the residue from ethyl acetate- hexane gave the desired product 1-24 in a form of white powder(84%). For characterization see Tabe 2 (Example 7). Example 4 N α -Nsc-N(4, 4 '-Dimethoxybenzhydryl) -Asparagine (1-17) . 3.89 g of N α -Nsc-asparagine and 2.6 g of 4,4'- dimethoxybenzhydrol were dissolved in 25 ml of glacial acetic acid. To the solution 0.4 ml of methanesulfonic acid was added, and the mixture was allowed to stand overnight at room temperature. The resulting mixture was then poured into 100 ml of ice-cold water with mixing, the formed precipitate was filtered off, washed with water and them with ether. The crude product was dissolved in 10 ml of warm DMF, filtered and reprecipitated with ether. The precipitate was collected by filtration, washed with ether and dried in vacuo yielding the desired compound 1-17 as a crystalline power (80%). For characterization see Table 2 (Example 7). Example 5 N α -Nsc-Arginine ( 1 -13 ) . To the solution of 1 . 7 g of sodium bicarbonate and 4 .22 g of arginine hydrochloride in 25 ml of water stirred at room temperature 5. 74 g of 2- ( 4-nitrophenylthio ) ethylchloro-formate was added in five equal portions during 30 min, then stirring was continued for additional 2 hrs . The mixture was saponified to pH 8.5 with cone, ammonia solution and left for 3 hrs at 4°C. Precipitate formed was collected, washed with ice-cold water, dried on the filter with suction and dissolved in 30 ml of acetic acid. To the solution 4 ml of 34% hydrogen peroxide was added, and the mixture was left for 30 hrs at room temperature. Then the miture was evaporated to dryness, and the residue was triturated with ethyl acetate, collected by fitration, washed with ether and dried on air to give the desired product 1-13(88%). For characterization see Table 2 (Example 7) . Example 6 N α -Nsc-Histidine(I-4) . 6.6 g of N α -Nsc-N im -triphenylmethyl-histidine was dissolved in 30 ml 90% aqueous acetic acid and boiled under reflux for 30 min. Ater cooling the precipitate was filtered off, and the filtrate was evaporated. The residue was triturated with ether and collected by filtration giving the desired compound 1-4 in 95% yield. For characterization see Table 2 (Example 7). Example 7 Properties of N α -Nsc-amino acids I. Shown in Tabel 2 are the compounds of formula I which were prepared utilizing provided methods described in detail in examples 1-6. Figures in the column "Method" correspond to numbers of examples where particular methods are described. Chromatographic mobility values R f are shown for thin-layer chromatography sheets Alufolien Keselgel 60 F 254 (Merck, Darmstadt, Germany); chloroform/methanol/acetic acid, 95:5:3, (A) and benzene/acetone/acetic acid, 100:50:3, (B) , were used as developing solvents, spots were detected by UV-absorbance and/or by ninhydrin reaction. Molecular ion masses (M+H) + were measured using MS-BC-1 time-of-flight mass spectrometer with Cf 252 radiation-promoted desorption (Electrom SPA, Sumy, Ukraine) . Tabel 2 Properties of N α -Nsc-amino acids I 1-1 Nsc-Ser-OH 2 0.40 0.30 C, 2 H 14 N 2 O 9 S 363.32 363.6 1-2 Nsc-Thr-OH 2 0.42 0.35 C I3 H 16 N 2 O 9 S 377.35 377.6 1-3 Nsc-Tyr-OH 2 0.40 0.37 l8^il8^'2^'9^ 439.44 439.9 1-4 Nsc-His-OH 6 0.15 0.10 C 15 H 16 N 4 O 8 S 413.40 413.1 1-5 Nsc-Ser(Bzl)-OH 1,3 0.70 0.55 C 19 H 20 N 2 O 9 S 453.46 453.8 1-6 Nsc-Thr(Bzl)-OH 3 0.70 0.55 C 20 H 22 N 2 O 9 S 467.49 467.9 1-7 Nsc-Tyr(Bzl)-OH 1,3 0.75 0.55 C 25 H 24 N 2 O 9 S 529.57 530.1 1-8 Nsc-Asp(OBzl)-OH 3 0.60 0.45 C 20 H 20 N 2 O 10 S 481.47 480.9 1-9 Nsc-Asp(OBzl)-OH 3 0.62 0.43 C 21 H 22 N 2 O 10 S 459.50 495.1 1-10 Nsc-Cys(Bzl)-OH 3 0.75 0.60 C 19 H 2 oN 2 O 8 S 2 469.53 469.3 1-11 Nsc-Cys(Dpm)-OH 1,3 0.75 0.70 C 25 H 24 N 2 O 8 S 2 545.63 545.2 1-12 Nsc-Lys(Z)-OH 3 0.65 0.50 C 23 H 27 N 3 O 10 S 538.58 538.3 1-13 Nsc-Arg-OH 5 0.10 0.05 C 15 H 21 N 5 O 8 S 432.45 432.8 1-14 Nsc-Arg(Tos)-OH 1,2 0.35 0.30 C 22 H 27 N S O 10 S 2 586.64 586.9 1-15 Nsc-Arg(NO 2 )-OH 2 0.25 0.15 C 15 H 20 ,N 6 O 10 S 477.45 477.1 1-16 Nsc-Arg(Mtr)-OH 3 0.43 0.35 C 25 H 33 N 5 O 10 S 2 628.73 629.4 1-17 Nsc-Asn(Mbh)-OH 4 0.55 0.38 C 28 H 29 N 3 O n S 616.65 616.1 1-18 Nsc-Gln(Mbh)-OH 4 0.58 0.33 C 29 H 31 N 3 O n S 630.68 630.2 1-19 Nsc-Cys(StBu)-OH 3 0.60 0.45 467.57 567.2 1-20 Nsc-Lys(Nsc)-OH 3 0.55 0.40 C 24 H 28 N 4 O 14 S 2 661.66 662.1 1-21 Nsc-Nva-OH 2 0.65 0.50 C, 4 H 18 N 7 O s S 375.39 375.6 1-22 Nsc-Nle-OH 2 0.65 0.50 C 15 H 20 N 2 O 8 S 389.42 389.6 1-23 Nsc-Abu-OH 2 0.60 0.47 C 13 H 16 N 2 O 8 S 375.40 375.8 1-24 Nsc-Om(Z)-OH 3 0.62 0.47 C 22 H 25 N 3 O 10 S 533.55 534.0 1-25 Nsc-Om(Boc)-OH 3 0.65 0.43 C 19 H 27 N 3 O 10 S 490.53 491.0

Claims: What is claimed is: 1. N-2- (4-nitrophenylsulfonyl) ethoxycarbonyl-amino acids having the general formula: wherein R x represents hydrogen atom, and R 2 may represent hydroxymethyl, 1-hydroxyethyl, 4-hydroxybenzyl, imidazolyl-2- methyl, benzyloxymethyl, 1-benzyloxyethyl, 4-benzyloxybenzyl, benzyloxycarbonlmethyl, 2- (benzyloxycarbonyl) ethyl, S- benzylthiomethyl, S- (diphenylmethyl ) thiomethyl, 4- (benzyloxycarbamido) butyl, 3-guanidinopropyl, 3- (N G - toluenesulfonyl) guanidinopropyl, 3- (N G -nitro) guanidinopropyl, 3- [N G - (4-methoxy-2, 3, 6-trimethylbenzenesulfonyl) ] - guanidinopropyl , N- ( 4 , 4 ' -dimethoxybenzhydryl ) carboxamidomethyl , 2- [N- (4, '-dimethoxybenzhydryl) carboxamido] ethyl, S-tert- butyldithiomethyl, 4- [2- (4-nitrophenylsulfonyl) ethoxycarbamido] -butyl, propyl, butyl, ethyl, 3- (benzyloxycarbamido) propyl, or 3- (tert-butoxycarbamido) propyl, or 3- (tert-butoxycarbamido) - propyl . 2. A method for preparing of compounds according to the claim 1 comprising reacting amino acid of the general formula wherein R x and R 2 represent radicals according to the claim, with 2- (4-nitrophenylsulfonyl) ethoxycarbonyl chloroformate in mixed aqueous-organic solvent in the presence of base. 3. A method for preparing of compounds according to the claim 1 comprising: a) converting of amino acid of the general formula wherein R x and R 2 represent radicals according to the claim 1, into 0,N-trimethylsilylated derivatives; b) reacting of said 0,N-trimethylsilylated derivatives with 2- (4-nitrophenylsulfonyl) ethoxycarbonyl chloroformate in aprotic solvent in the presence of base with subsequent hydrolysis . 4. A method for preparing of compound according to the claim 1, wherein R x represents hydrogen atom, and R 2 represents N- (4, ' -dimethoxybenzhydryl) carboxamidomethyl or 2-[N-(4,4'- dimethoxybenzhydryl) carboxamido] ethyl, comprising reacting of N α -2- (4-nitrophenylsulfonyl) -ethoxycarbonyl-asparagine or N α -2- (4-nitrophenylsulfonyl) ethoxycarbonyl-glutamine, with 4-4'- dimethoxybenzhydol in organic solvent in the presence of acid. 5. A method for preparing of compound according to the claim 1, wherein R x represents hydrogen atom, and R 2 represents 3-guanidinopropyl, comprising reacting of arginine with 2- (4- nitro-phenylthio) ethylchloroformate in water in the presence of sodium bicarbonate and oxidizing thus formed N α -2-(4- nitrophenylthio) ethoxycarbonyl-arginine with hydrogen peroxide in organic solvent. 6. A method for preparing of compound according to the claim " !, wherein R x represents hydrogen atom, and R 2 represents imidazolyl-2-methyl, comprising reacting of N α -2-(4- nitrophenylsulfonyl) ethoxycarbonyl-N im - triphenylmethyl - histidine with acid.

Inventors: KIM HACK-JOO, KIM YOUNG-DEUG, POZDNYAKOV PAVEL I, SABIROV AYDAR N, SAMUKOV VLADIMIR V, KIM, HACK-JOO, KIM, YOUNG-DEUG, POZDNYAKOV, PAVEL I., SABIROV, AYDAR N., SAMUKOV, VLADIMIR V.
Applicants: HYUNDAI PHARM IND CO LTD, KIM HACK JOO, KIM YOUNG DEUG, POZDNYAKOV PAVEL I, SABIROV AYDAR N, SAMUKOV VLADIMIR V, HYUNDAI PHARM. IND. CO., LTD., KIM, HACK-JOO, KIM, YOUNG-DEUG, POZDNYAKOV, PAVEL I., SABIROV, AYDAR N., SAMUKOV, VLADIMIR V.
Application year: 1996
Main classification: C07C317
IPC codes: C07C315, C07C317, C07D233, C07K1