PEPTIDE, BRONCHODILATOR AND BLOOD FLOW AMELIORANT

Abstract: Peptides represented by the following general formula (1) or pharmaceutically acceptable salts thereof, and a bronchodilator or a blood flow ameliorant containing the same as the active ingredient: H-His-Ser-Asp-A-B-Phe-Thr-Asp-C-Tyr-D-Arg-E-Arg-F-Gln-G-Ala-Val-I-J-Tyr-Leu-K-L-M-Leu-N, wherein A represents Ala or Gly; B represents Ile or Val; C represents Asn or Ser; D represents Thr or Ser; E represents Leu or Tyr; F, I and J represent each Lys or Arg, provided that at least one of F, I and J is Arg; G represents Met, Leu or Nle; K represents Asn or Ala: L represents Ser or Ala; M represents Ile or Val; and N represents -NH 2 or Asn-NH 2 , excluding the case where A is Ala, B is Val, C is Asn, D is Thr, E is Leu, K is Asn, L is Ser, M is Ile and N is Asn-NH 2 at the same time.

Description: The present invention relates to a novel peptide, a bronchus-expanding agent and a blood-flow-improving agent. VIP (vasoactive intestinal peptide) is one kind of physiologically active peptide called brain-gut peptide, the action of which is to promote blood flow and depress blood pressure. VIP was extracted from the intestine of a pig in 1970 and it consists of 28 amino acid residues (S. I. Said, V. Mutt Science, 169, 1217 (1970)). PACAP (pituitary adenylate cyclase activating polypeptide) was isolated from the hypothalamus of a sheep in 1989 in a bioassay system using as an indicator the activation of adenylate cyclase from cultured pituitary cells, and it is a structurally determined peptide consisting of 38 amino acid residues (A. Miyata, A. Arimura et al., Biochem. Biophys. Res. Commun., 164 , 567 (1989)). 27 amino acid residues from the N-terminal side of PACAP has the PACAP activity and the amino acid sequence of said 27 amino acids has a considerably similar structure to that of VIP. VIP and PACAP are similar in amino acid sequence to secretin, glucagon etc. and are thus classified as peptides belonging to the glucagon family. By virtue of their strong action on vessel expansion and blood-flow promotion, VIP and PACAP are expected for use in the treatment of an ulcer, frostbite, bedsore, impotence etc. and in the restoration and growth of hair. In addition, VIP and PACAP have considerably strong relaxation action on the bronchial smooth muscle in the respiratory system, thus alleviating the smooth muscle contraction induced by stimulators such as acetylcholine, histamine, serotonin etc. Such relaxation action of VIP and PACAP differ from the relaxation action caused via adrenalin β 2 receptor by general bronchial expansion, so VIP and PACAP are expected to have a treatment effect on asthmatic spasm difficult to cure on which a stimulator of β 2 receptor does not effectively act. Besides VIP and PACAP, a peptide consisting of 28 to 31 amino acid residues with bronchus expanding action, blood-pressure reducing action and hair restoring action is known (see Japanese Patent Application Laid-Open publication Nos. 246,595/87, 83,012/89 and 297,498/92). The prior art also provides VIP analogues having a residue Met at position 17 exhibiting strong blood flow increasing action, low hypotensive side effects and being effective for ulcer, chilblain, impotency and hair-growth (JP4-297 498; WPI Database: Derwent Publications LTD-AN:1992-401811). VIP analogues having a hypertension reducing or a bronchoconstriction reducing activity are also disclosed in WO 88/06598. It is however desired to develop a bronchus-expanding agent and a blood-flow-improving agent superior to VIP, PACAP and other peptides in the durability of bronchus-expanding action and blood-flow increasing action. The object of the present invention is to provide a novel peptide excellent in the durability of bronchus-expanding action and further excellent in blood-flow increasing action, a bronchus-expanding agent and a blood-flow-improving agent comprising said peptide as an active ingredient. The present invention provides a peptide represented by the formula: or or the general formula (1): wherein A is Ala or Gly; B is lie or Val; C is Asn or Ser, D is Thr or Ser; E is Leu or Tyr; each of F, I and J is Lys or Arg, and at least one of F, I and J is Arg; G is Met, Leu or nLeu; K is Asn ar Ala; L is Ser or Ala; M is Ile or Val; in which at least one of F, I, and J is Arg; P is Asn or a chemical bond; Q is Lys, Arg, Lys-Arg, Arg-Arg or a chemical bond; and R is -OH or -NH 2 , or pharmaceutically acceptable salts thereof. Further, the present invention provides a bronchus-expanding agent comprising said peptide or a pharmaceutically acceptable salt thereof as an active ingredient. Further, the present invention provides a blood-flow-improving agent comprising said peptide or a pharmaceutically acceptable salt thereof as an active ingredient. Further, the present invention provides a pharmaceutical composition comprising said peptide or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier. Further, the present invention provides use of said peptide or a pharmaceutically acceptable salt thereof for the preparation of a pharmaceutical composition for expanding a bronchus. Further, the present invention provides use of said peptide or pharmaceutically acceptable salt thereof for the preparation of a pharmaceutical composition for improving a blood flow. The peptide of the invention or a salt thereof is excellent in the durability of bronchus-expanding action resulting from the relaxation action of smooth muscle. Hence, the peptide of the invention and a salt thereof is useful as a bronchus-expanding agent. In addition, the peptide of the invention or a salt thereof is excellent in blood-flow-increasing action and in its durability. Therefore, the peptide of the invention or a salt thereof is also useful as a blood-flow-improving agent and it can exert an improvement effect on a blood flow at a low dosage. The abbreviations of amino acids, peptides, protective groups, solvents etc., in the present specification follow those according to International Union of Pure and Applied Chemistry (IUPAC) or International Union of Biochemistry (IUB) or conventional abbreviations in this field. Examples are shown below. If there can be optical isomers for amino acids etc., L-isomers are meant unless otherwise specified. His: histidine residue Ala: alanine residue Thr: threonine residue Leu: leucine residue Gln: glutamine residue Gly: glycine residue Boc: t-butyloxycarbonyl group Bzl: benzyl group Tos: p-toluenesulfonyl group OMe: methylester ONP: p-nitrophenylester TFA: trifluoroacetic acid Ser: serine residue Val: valine residue Tyr: tyrosine residue Arg: arginine residue Met: methionine residue Nle: norleucine residue Asp: aspartic acid residue Phe: phenylalanine residue Asn: asparagine residue Lys: lysine residue Ile: isoleucine residue Aoc: t-amyloxycarbonyl group z: benzyloxycarbonyl group OBut: t-butylester OBz: benzylester Bom: benzyloxymethyl group THF: tetrahydrofuran DCM: dichloromethane DCC: dicyclohexylcarbodiimide WSC: N-ethyl-N'-dimethylaminopropyl-carbodimimide OSu: N-hydroxysuccinimide ester HOSu: N-hydroxysuccinimide HOBt: 1-hydroxybenzotriazole DIEA: diisopropylethylamine. DMF: dimethylformamide The peptides represented by the above formulas can be produced by conventional means for synthesis of known peptides. For example, these peptides can be produced in e.g. an azide method, acid chloride method, acid anhydride method, mixed acid anhydrides - method, DCC method, active ester method (p-nitrophenylester method, N-hydroxysuccinic acid imide ester method, cyanomethylester method etc.), a method using Woodward reagent K, carboimidazole method, oxidation-reduction method, DCC-additive (HONB, HOBt, HOSu) method etc. according to methods described in e.g. "The Peptides" Vol. 1 (1966) [Schreder and Luhke: Academic Press, New York, U.S.A.] or "Peputido Gosei" (Peptide Synthesis) [Izumiya et al., Maruzen K.K. (1975), Japan]. These methods can be applied to both solid-phase synthesis and liquid-phase synthesis. According to the above-described methods for synthesis of conventional polypeptides, a target peptide can be produced in a stepwise elongation method where each of the amino acids in said peptide is linked sequentially to the C-terminal amino acid or in a fragment condensation method where partial fragments in said peptide are synthesized and then coupled to one another. For example, the solid-phase synthesis in the stepwise elongation method can be carried out in accordance with the method of Merrifield, R.B. (Solid Phase Peptide Synthesis, J. Amer. Chem. Soc., 85 , 2149-2159 (1963)) as follows: The C-terminal amino acid of a target peptide (that is, in the present invention, the amino acid sequence of the above formulas is protected on its amino group and then coupled to insoluble resin having functional groups capable of binding to carboxyl groups. The amino group is then deprotected, and to this free amino group is bound the next amino acid with its amino group protected. These steps are repeated many times, thus elongating the amino acid chain until the histidine residue at the 1-position of the above, formulas. The peptide thus obtained is then cleaved off from the resin. The above method requires the protection (that is, linking a protective group to the amino group) and the deprotection (that is, releasing the protective group from the amino group) for the amino group participating in peptide bonding between amino acids as well as the activation of the carboxyl group participating in peptide bonding between amino acids. If necessary, a protective group is further linked to a functional group in a side chain of the amino acid. The protective group used in protecting the amino group includes those conventionally used. Examples are benzyloxycarbonyl (z), t-butyloxycarbonyl (Boc), t-amyloxycarbonyl (Aoc), isobornyloxycarbonyl, p-methoxybenzyloxycarbonyl, 2-chlorobenzyloxycarbonyl, amadantyloxycarbonyl, trifluoroacetyl, phthaloyl, formyl, o-nitrophenylsulphenyl, and diphenylphosphinothioyl. The protective group used in protecting the carboxyl group includes those conventionally used. Examples are alkyl esters such as methyl ester, ethyl ester, propyl ester, butyl ester and tert-butyl ester, benzyl ester, p-nitrobenzyl ester, methylbenzyl ester, p-chlorobenzyl ester, benzhydryl ester, benzyloxycarbonylhydrazide, tert-butyloxycarbonylhydrazide and tritylhydrazide. The activation of the carboxyl group participating in peptide bonding can be carried out in any of the conventionally known methods described above, and the reagents etc. used in the activation can be selected from known ones. The activation of the carboxyl group involves reacting various reagents with said carboxyl group to form e.g. their corresponding acid chlorides, acid anhydrides or mixed acid anhydrides, azides, and active esters (for example, esters such as pentachlorophenol, p-nitrophenol, N-hydroxysuccinimide, N-hydroxybenztriazole, N-hydroy-5-norbornen-2,3-dicarboxyimide etc.). For the amino acid with a functional group in its side chain, the functional group is preferably protected during the reaction of forming a peptide bond. In particular, His, Tyr, Thr, Lys, Asp, Arg and Ser are preferably protected at the functional group in their side chain. The protection of the functional group is carried out by linking the protective group to it in a conventional method. After the completion of peptide synthesis, the protective group is released. The protective group used in protecting the imino group in His includes e.g. benzyloxymethyl (Bom), tosyl (Tos), benzyl (Bzl), benzyloxycarbonyl (Z) and trityl. The hydroxyl group in Ser and Thr can be protected by esterification or etherification, but its protection is not necessarily required. The protective group to be introduced by esterification includes e.g. lower alkanoyl groups such as acetyl etc., aroyl groups such as benzoyl etc., and carbonic acid-derived groups such as benzoyloxycarbonyl, ethyloxycarbonyl etc. Preferable examples of protective groups introduced by etherification are benzyl (Bzl), tetrahydropyranyl and tert-butyl. The protective group used in protecting the hydroxyl group in Tyr includes e.g. benzyl (Bzl), bromobenzyloxycarbonyl (BrZ), dichlorobenzyl (Cl 2 -Bzl), benzyloxycarbonyl (Z), acetyl, and tosyl (ToS). The protective group used in protecting the amino group in Lys includes e.g. benzyloxycarbonyl (Z), chlorobenzyloxycarbonyl (Cl-Z), dichlorobenzyl (Cl 2 -Bzl), t-butyloxycarbonyl (Boc), and tosyl (Tos). The protective group used in protecting the guanidino group in Arg includes e.g. tosyl (Tos), nitro, benzyloxycarbonyl (Z), and t-amyloxycarbonyl (Aoc). The protection of the carboxyl group in Asp is carried out by esterification with e.g. benzyl alcohol, methanol, ethanol or tert-butanol. The reaction of forming a peptide bond can sometimes be carried out in the presence of a condensation reagent e.g. a carbodiimide reagent such as dicyclohexylcarbodiimide (DCC), carbodiimidazole etc., or tetraethyl pyrophosphate, benzotriazole-N-hydroxytrisdimethylaminophosphonium hexafluorophosphate (Bop reagent). The insoluble resin used in the above solid-phase synthesis may be any resin having functional groups capable of binding to carboxyl groups. Examples are benzhydrylamine resin (BHA resin), chloromethyl resin, oxymethyl resin, aminomethyl resin, p-methylbenzhydrylamine resin (MBHA resin), 4-aminomethylphenoxymethyl resin, 4-hydroxymethylphenoxymethyl resin, 4-oxymethylphenylacetamidem ethyl resin (PAM resin) etc. The linking of the amino acid to the resin and cleavage of the synthesized peptide from the resin can be carried out in any of the conventionally known methods. The solvent used in the above solid-phase synthesis may be any solvents known to be usable in forming a peptide bond, and examples are water-free or water-containing dimethylformamide (DMF), dimethyl sulfoxide (DMSO), pyridine, chloroform, dioxane, dichloromethane (DCM), tetrahydrofuran (THF), ethyl acetate, N-methylpyrrolidone, and hexamethyl phosphate triamide (HMPA). These solvents can be used singly or in combination. The peptide thus prepared can be desalted and purified in a usual manner. For example, it can be desalted and purified by ionexchange chromatography on DEAE-cellulose etc., partition chromatography on Sephadex LH-20, Sephadex G-25 etc., normal phase chromatography on silica gel etc., reverse phase chromatography on ODS-silica gel etc., or in high performance liquid chromatography (HPLC), etc. The pharmaceutically acceptable salts of the peptides represented by the above formulas include e.g. acetate, hydrochloride, phosphate etc. The peptide of the invention and salts thereof are useful as a bronchus-expanding agent by virtue of their bronchus-expanding action. Hence, the bronchus-expanding agent containing the peptide of the invention or a salt thereof as an active ingredient is useful in suppression and improvement of asthma etc. As the bronchus-expanding agent, the peptide of the invention or a salt thereof can be administered as such, but usually it is mixed in a usual manner with various pharmaceutically acceptable carriers to be formed into preparations in the form of liquid, gel or solid, for oral or parenteral purposes. The administration method includes inhalation (e.g. with an aerosol agent), injection, application etc. The dosage of the present peptide or a salt thereof when used as a bronchus-expanding agent is preferably in the range of about 1 ng/kg to 1 mg/kg (body weight)/day/person, although the dosage is determined depending on the purpose for use, symptom, the age and body weight etc. of a patient, method of administration, etc. Further, the peptide of the invention or a salt thereof is excellent in blood-flow increasing action which is superior in durability. Hence, a blood-flow improving agent comprising the peptide of the invention or a salt thereof is effective for treatment of an ulcer, frostbite, bedsore, impotence etc. as well as for restoration and growth of hair. The peptide of the invention or a salt thereof when used as a blood-flow improving agent is mixed with a conventional pharmaceutically acceptable carrier to be formed into pharmaceutical preparations, preferably in the form of an injection. For preparation of such an injection, the resulting preparation is preferably sterilized and isotonic relative to blood. Any conventionally used diluents can be used for manufacture of a preparation in the form of an injection. Example of diluents are water, physiological saline etc. In this case, a sufficient amount of common salt or glycerin to prepare an isotonic solution can be contained in a preparation in the form of an injection. This preparation can contain a conventional buffer, soothing agent, preservative etc. and if necessary a coloring agent, preservative, flavoring agent, sweetener, etc., as well as other pharmaceuticals. The above active ingredient for injection purposes can be dissolved before use in distilled water. The method of administering the resulting pharmaceutical preparation is varied depending on the form of the preparation. For example, the preparation in the form of an injection is intravenously administered singly or after being mixed with conventional complements such as amino acids etc. The present peptide or a salt thereof when used as a blood-flow-improving agent is administered preferably in 2 to 4 portions daily in the range of about 1 ng/kg to 1 mg/kg (body weight) /day/person, although the dosage is determined depending on the purpose for use, symptom conditions, etc. FIG. 1 is a graph showing a change with time in the degree of relaxation of bronchial smooth muscle in the case of administration of PACAP. FIG. 2 is a graph showing a change with time in the degree of relaxation of bronchial smooth muscle in the case of administration of Peptide 5 shown in SEQ ID NO:5. FIG. 3 is a graph showing a change with time in the degree of relaxation of bronchial smooth muscle in the case of administration of VIP. FIG. 4 is a graph showing a change with time in the degree of relaxation of bronchial smooth muscle in the case of administration of Peptide 11 shown in SEQ ID NO:11. Hereinafter, the present invention is described in detail by reference to Examples, which however are not intended to limit the scope of the present invention. In the following examples, the identification of the resulting purified peptide was carried out by measurement of retention time in high performance liquid chromatography (HPLC), measurement of degree of optical rotation, and amino acid analysis, as shown below. • High performance liquid chromatography (HPLC) For analysis in high performance liquid chromatography, LC-Module-1 (Waters Ltd. Japan) was used. (Analysis conditions in HPLC) Column : TSK Gel ODS-120T (4.6×250 mm) Solvent: 0.1 % TFA-acetonitrile (linear gradient of from 20 % to 50 % acetonitrile for 30 min). Flow rate: 1 ml/min. Detection wavelength: 220 nm. • Degree of optical rotation For measurement of degree of optical rotation, DIC-370 (Nippon Bunko Kogyo K.K., Japan) was used. (Conditions for measurement of degree of optical rotation) Light source: Na lamp, 589 nm. Temperature: 20°C . Layer length: 100 mm. Concentration: 1 (0.1 M in acetic acid). • Amino acid analysis: The amino acid analysis was carried out by hydrolyzing a peptide at 110 °C for 20 hours in 6 N HCl containing 0.1 % phenol and analyzing the hydrolysate in Hitachi Amino Acid Analyzer L-8500 (Hitachi, Ltd., Japan). Example 1 Production of peptide 1 The solid-phase synthesizer used was Peptide Synthesizer 9600 (Milligen Bioresearch). Peptide 1 shown in SEQ ID NO:1 whose C-terminal group was amidated was produced by the following solid-phase synthesis and purification. First, 694 mg MBHA resin (0.72 mmol/g amino group, manufactured by Peptide Kenkyuzyo K.K., Japan) was introduced into a reactor for peptide solid-phase synthesis and treated with 8 ml DCM (4 times, 1 minute each), 8 ml DCM containing 60 % TFA (20 minutes), 4 ml DCM (3 times, 15 seconds each), 3 ml DMF solution containing 1 ml DIEA (2 times, 1 minute each), and 8 ml DMF (6 times, 40 seconds each) in this order with stirring in an argon stream. After each treatment, filtration was carried out. Separately, 2 mmol amino-group-protected amino acid Boc-Leu-OH corresponding to the amino acid residue at the 27-position in SEQ ID NO:1 was dissolved in 4 ml DCM. This solution was introduced into a vessel for amino acid activation, and after 3 ml DCC (0.5 M in DCM) and 4 ml HOBt (0.5 M in DCM) were added to it, and they were reacted for 30 minutes. Thereafter, the reaction solution was filtered off and the filtrate was transferred to a concentration vessel. 3 ml DMF was added to it and the DCM was distilled off in an argon stream. Then, 3 ml DMF was added to the concentrate, and it was transferred to the above reactor for peptide solid-phase synthesis and reacted for 30 minutes. Then, the reaction mixture was washed with 8 ml DCM (6 times, 20 seconds each). Separately, 2 mmol Boc-Leu-OH was dissolved in 4 ml DCM and the solution was introduced into the reactor for amino acid activation and the same procedure as described above was repeated (double-coupling) and filtered. Boc-Leu-MBHA resin was thus obtained. Then, the Boc-Leu-MBHA resin was washed with 8 ml DCM (4 times, 1 minute each) and filtered. This resin was treated with 8 ml DCM containing 60 % TFA (20 minutes), 4 ml DCM (3 times, 15 seconds each) , 3 ml DMF solution containing 1 ml DIEA (2 times, 1 minute each), and 8 ml DMF (6 times, 40 seconds each) in this order with stirring in an argon stream. After each treatment, filtration was carried out. Separately, 2 mmol amino-group-protected amino acid Boc-Val-OH corresponding to the amino acid residue at the 26-position in SEQ ID NO:1 was dissolved in 4 ml DCM and introduced into a vessel for amino acid activation, and after 1.5 ml DCC (0.5 M in DCM) was added to it, and they were reacted for 7 minutes. Thereafter, the reaction solution was filtered off and the filtrate was transferred to a concentration vessel. 3 ml DMF was added to it and the DCM was distilled off in an argon stream. Then, 3 ml DMF was added to the concentrate, and it was transferred to the above reactor for peptide solid-phase synthesis and reaction was carried out for 30 minutes. Then, the reaction mixture was washed with 8 ml DCM (6 times, 20 seconds each) and filtered. Boc-Val-Leu-MBHA resin was thus obtained. Then, the following amino-group-protected amino acids, corresponding to the 25th to 1st amino acids in SEQ ID NO:1 respectively, were coupled successively to it. Amino Acid Protected Amino Acid Amount No. (mmol) 25 Boc-Ala-OH 2 24 Boc-Ala-OH 2×2 23 Boc-Leu-OH 2 22 Boc-Tyr(Bzl)-OH 2 21 Boc-Arg(Tos)-OH 2×2 20 Boc-Arg(Tos)-OH 2×2 19 Boc-Val-OH 2 18 Boc-Ala-OH 2 17 Boc-Leu-OH 2 16 Boc-Gln-OH 2×2 15 Boc-Arg(Tos)-OH 2×2 14 Boc-Arg(Tos)-OH 2×2 13 Boc-Tyr(Bzl)-OH 2 12 BOC-Arg(Tos)-OH 2×2 11 Boc-Ser (Bzl) OH 2 10 Boc-Tyr(Bzl)-OH 2 9 Boc-Ser(Bzl)-OH 2 8 Boc-Asp(OBz)-OH 2 7 Boc-Thr(Bzl)-OH 2 6 Boc-Phe-OH 2 5 Boc-Ile-OH 2 4 Boc-Gly-OH 2 3 Boc-Asp(OBz)-OH 2 2 Boc-Ser(Bzl)-OH 2 1 Boc-His(Bom)-OH 2×2 In the above solid-phase synthesis, double coupling was carried out in the case of Asn, Arg, Gln and His. 2.76 g of the following protected peptide-HBHA resin was thus obtained: Boc-His(Bom)-Ser(Bzl)-Asp(OBz)-Gly-Ile-Phe-Thr(Bzl)-Asp(OBz)-Ser(Bzl)-Tyr(Bzl)-Ser(Bzl)-Arg(Tos)-Tyr(Bzl)-Arg(Tos)-Arg(Tos)-Gln-Leu-Ala-val-Arg(Tos)-Arg(Tos)-Tyr(Bzl)-Leu-Ala-Ala-Val-Leu-MBHA resin. 5 ml anisole was added to 2.76 g of the above protected peptide-MBHA resin, and 25 ml anhydrous hydrogen fluoride was added to it and the mixture was reacted with stirring at 0°C for 1 hour. Thereafter, the anhydrous hydrogen fluoride was distilled off under reduced pressure. The residue was washed with ether and then the peptide was extracted from it with 100 ml of 0.1 M acetic acid. The extract was stirred for 15 minutes together with 20 ml anion-exhange resin Amberlite IR-410 and the insoluble resin was removed by filtration. The suspension thus obtained was filtered through 0.22µ Millipore filter and the filtrate was lyophilized. 823 mg white powder was thus obtained. Then, this sample was applied to a column of CM-cellulose (2.5×30 cm) and eluted with a linear gradient of from 0.05 M to 0.5 M AcONH 4 (pH 7.0), and fraction Nos. 69 to 82 (10 ml/fraction) were combined and gave 382 mg of the desired peptide in partially purified form. This peptide was further purified in preparative high performance liquid chromatography. Column: TSK Gel ODS-120T (21.5×300 mm). Solvent: 0.1 % TFA-acetonitrile (linear gradient of from 20 % to 40 % acetonitrile). Flow rate: 10 ml/min. The eluate corresponding to a peak of peptide 1 as the desired substance was lyophilized. The peptide in purified form was obtained in said HPLC in an amount of 63 mg per 100 mg of the partially purified peptide. The purified peptide 1 was determined for retention time in HPLC and for degree of optical rotation, and its amino acids were analyzed. The results are shown below: Retention time in HPLC: 27.2 min. [α] D : -55.8° Amino acid analysis: Asp(2) 2.17, Thr(1) 0.98, Ser(3) 2.45, Glu(1) 1.09, Gly(1) 1.03, Ala(3) 3.18, Val(2) 2.14, Ile(1) 0.96, Leu(3) 3.07, Tyr(3) 2.73, Phe(1) 0.92, His(1) 1.12, Arg(5) 5.16. Example 2 Peptide 2 shown in SEQ ID NO:2 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 73 mg Retention time in HPLC: 27.6 min. [α ] D : -53.2° Amino acid analysis: Asp(2) 2.10, Thr(1) 0.97, Ser(3) 2.46, Glu(1) 0.97, Gly(2) 1.97, Ala(3) 3.15, Val(2) 2.13, Ile(1) 1.00, Leu(3) 3.08, Tyr(3) 2.75, Phe(1) 0.98, His(1) 0.99, Arg(5) 4.54. Example 3 Peptide 3 shown in SEQ ID NO:3 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 88 mg Retention time in HPLC: 27.0 min. [α ] D : -52.8° Amino acid analysis: Asp(2) 2.11, Thr(1) 0.95, Ser(3) 2.56, Glu(1) 0.97, Gly(2) 2.00, Ala(3) 3.15, Val(2) 2.10, Ile (1) 1.00, Leu(3) 3.08, Tyr(3) 2.75, Phe(1) 0.98, His(1) 1.03, Arg(5) 5.14, Lys(1) 1.01. Example 4 Peptide 4 shown in SEQ ID NO:4 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 84 mg Retention time in HPLC: 25.1 min. [α] D : -54.3° Amino acid analysis: Asp(2) 2.09, Thr(1) 0.96, Ser(3) 2.44, Glu(1) 0.97, Gly(2) 2.01, Ala(3) 3.11, Val(2) 2.03, Ile(1) 1.02, Leu(3) 3.08, Tyr(3) 2.74, Phe(1) 0.97, His(1) 1.05, Arg(6) 6.10 Example 5 Peptide 5 shown in SEQ ID NO:5 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 76 mg Retention time in HPLC: 24.4 min. [α ] D : -52.2° Amino acid analysis: Asp(2) 2.11, Thr(1) 0.96, Ser(3) 2.47, Glu(1) 1.01, Gly(2) 2.00, Ala(3) 3.03, Val(2) 2.03, Ile(1) 1.02, Leu(3) 3.03, Tyr(3) 2.70, Phe(1) 0.97, His(1) 1.07, Arg(6) 6.15, Lys(1) 1.02. Example 6 Peptide 6 shown in SEQ ID NO:6 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 85 mg Retention time in HPLC: 23.6 min. [α ] D : -53.7° Amino acid analysis: Asp(2) 2.09, Thr(1) 0.94, Ser(3) 2.51, Glu(1) 1.05, Gly(2) 2.00, Ala(3) 3.03, Val(2) 2.01, Ile(1) 1.02, Leu(3) 3.03, Tyr(3) 2.77, Phe(1) 0.97, His(1) 1.07, Arg(7) 7.11. Example 7 Peptide 7 shown in SEQ ID NO:7 was obtained in purified form in the same manner as in Example 1 except that 704 mg of Boc-Arg(Tos)-PAM resin (0.68 mmol/g amino group, produced by Peptide Kenkyuzyo K.K.), i.e. a resin having the C-terminal amino acid residue of the target peptide, was used. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 84 mg Retention time in HPLC: 23.8 min. [α] D : -56.7° Amino acid analysis: Asp(2) 2.12, Thr(1) 0.96, Ser(3) 2.47, Glu(1) 1.01, Gly(2) 2.00, Ala(3) 3.03, Val(2) 2.03, Ile(1) 1.02, Leu(3) 3.03, Tyr(3) 2.70, Phe(1) 0.97, His(1) 1.03, Arg(6) 6.10, Lys(1) 1.02. Example 8 Peptide 8 shown in SEQ ID NO:8 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 68 mg Retention time in HPLC: 26.9 min. [α] D : -58.7° Amino acid analysis: Asp(5) 5.11, Thr(2) 1.72, Ser(2) 1.61, Glu(1) 1.10, Ala(2) 2.00, Val(2) 2.00, Ile(1) 1.02, Leu(4) 4.15, Tyr(2) 1.86, Phe(1) 0.99, His(1) 0.95, Arg(5) 4.75. Example 9 Peptide 9 shown in SEQ ID NO:9 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 76 mg Retention time in HPLC: 25.4 min. [α] D : -52.9° Amino acid analysis: Asp(5) 5.11, Thr(2) 1.83, Ser(2) 1.49, Glu(1) 1.09, Gly(1) 1.04, Ala(2) 2.01, Val(2) 2.01, Ile(1) 0.96, Leu(4) 4.19, Tyr(2) 2.03, Phe(1) 1.01, His(1) 1.07, Arg(6) 6.10, Lys(1) 1.05. Example 10 Peptide 10 shown in SEQ ID NO:10 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 77 mg Retention time in HPLC: 24.9 min. [α] D : -52.5° Amino acid analysis: Asp(5) 5.12, Thr(2) 1.83, Ser(2) 1.55, Glu(1) 1.08, Gly(1) 1.03, Ala(2) 2.01, Val(2) 2.01, Ile(1) 0.96, Leu(4) 4.17, Tyr(2) 2.03, Phe(1) 1.01, His(1) 1.03, Arg(6) 6.15. Example 11 Peptide 11 shown in SEQ ID NO:11 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 87 mg Retention time in HPLC: 23.5 min. [α] D : -55.5° Amino acid analysis: Asp(5) 5.11, Thr(2) 1.83, Ser(2) 1.49, Glu(1) 1.09, Gly(1) 1.04, Ala(2) 2.01, Val(2) 2.01, Ile(1) 0.96, Leu(4) 4.19, Tyr(2) 2.03, Phe(1) 1.01, His(1) 1.07, Arg(6) 6.10, Lys(1) 1.05. Example 12 Peptide 12 shown in SEQ ID NO:12 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield; 78 mg Retention time in HPLC: 23.0 min. [α] D : -56.2° Amino acid analysis: Asp(5) 5.13, Thr(2) 1.84, Ser(2) 1.49, Glu(1) 1.08, Gly(1) 1.03, Ala(2) 2.01, Val(2) 2.01, Ile(1) 0.96, Leu(4) 4.19, Tyr(2) 2.03, Phe(1) 1.01, His(1) 1.02, Arg(7) 7.11. Example 13 Peptide 13 shown in SEQ ID NO:13 was obtained in purified form in the same manner as in Example 1 except that 704 mg of Boc-Arg(Tos)-PAM resin, i.e. a resin having the C-terminal amino acid residue of the target peptide, was used. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 83 mg Retention time in HPLC: 22.8 min. [α] D : -58.6° Amino acid analysis: Asp(5) 5.15, Thr(2) 1.83, Ser(2) 1.53, Glu(1) 1.09, Gly(1) 1.01, Ala(2) 2.01, Val(2) 2.01, Ile(1) 1.02, Leu(4) 4.10, Tyr(2) 1.86, Phe(1) 1.00, His(1) 0.95, Arg(6) 6.15. Example 14 Peptide 14 shown in SEQ ID NO:14 whose C-terminal carboxyl group was amidated was obtained in purified form in the same manner as in Example 1. The yield of this purified peptide is shown below. Its retention time in HPLC and its degree of optical rotation were determined, and its amino acids were analyzed. The results are shown below: Yield: 62 mg Retention time in HPLC: 26.2 min. [α] D : -52.5° Amino acid analysis: Asp(2) 2.11, Thr(1) 0.95, Ser(3) 2.45, Glu(1) 1.04, Gly(1) 1.03, Ala(3) 3.18, Val(2) 2.14, Ile(1) 0.96, Leu(2) 2.10, Tyr(3) 2.73, Phe(1) 0.96, His(1) 1.02, Arg(5) 5.16, nLeu(1) 1.02. Example 15 Purified peptides 1 to 14 obtained in Examples 1 to 14 were examined for their bronchus-expanding action in the following manner and compared with that of VIP and PACAP. Male guinea pigs each weighing 450 to 500 g were cut at the jugular veins to open the chests, and the tracheae were immediately removed. Their tracheae were immediately placed in a Krebs solution and cut into loops and then formed into a chain by linking the cartilage parts with one another via a steel string. Then, the cartilage parts in the opposite side to the smooth muscular parts were cut such that the smooth muscular parts were attached to one another. The resulting sample was placed in an about 10-ml isothermic organ bath and perpendicularly suspended. The lower part was fixed and the upper part was connected to a grass force transducer with a load of 1.4 g. A gas of 94 % oxygen and 6 % carbon dioxide was sufficiently blown into this sample while a Krebs solution containing 0.1µ mol/l carbachol was dropped to it at a rate of 0.33 ml/min. to determine relaxation action. Because the relaxation reaction occurs depending on the content of a test sample in this drop solution, this can be used to compare the relaxation action of VIP and PACAP and that of the peptides of the invention on the bronchial smooth muscle. A solution of each of VIA, PACAP and the peptides of the invention was prepared in a concentration as high as 100-fold than the final concentration so that its concentration in the organ bath could be adjusted. 30 minutes after the carbachol-containing Krebs solution was dropped, 100 µl of the sample solution was dropped. It was assumed that the degree of contraction of the smooth muscle is 0 in the absence of carbachol and the degree is 100 in the presence of carbachol. Minimum contraction degree A (i.e. maximum relaxation value) in the presence of the sample was determined, and maximum relaxation degree B was calculated as follows: Maximum relaxation degree B (%) = 100 - A The time required to reach half of the maximum relaxation value (referred to hereinafter as "half-time T") after the addition of the sample was determined. A change in the relaxation degree of the bronchial smooth muscle with time was determined using each peptide at 3 dosages of 0.3 µM, 1 µ M, and 3 µ M respectively. The results are shown in the drawings: PACAP in FIG. 1; peptide 5 obtained in Example 5, in FIG. 2; VIP in FIG. 3; and peptide 11 obtained in Example 11, in FIG. 4. The maximum relaxation degree B at a dosage of 3 µ M is 75 % for peptide 5 and 80 % for peptide 11, and half-time T is 360 minutes or more in both the cases. From these results, the peptides of the invention, while possessing a similar relaxation effect to those of VIP and PACAP, exhibit superior durability, i.e. a longer working period than those of VIP and PACAP. Table 1 shows maximum relaxation degree B and half-life T for each peptide at each dosage. 0.3 µM 1 µM 3 µM B (%) T (min) B (%) T (min) B (%) T (min) PACAP 40 60 90 60 90 100 VIP 45 130 80 150 85 240 Peptide 1 in Example 1 50 100 80 240 85 >360 Peptide 2 in Example 2 55 120 80 240 85 >360 Peptide 3 in Example 3 55 120 80 240 85 >360 Peptide 4 in Example 4 55 120 80 240 85 >360 Peptide 5 in Example 5 60 120 80 >360 75 >360 Peptide 6 in Example 6 65 150 80 >360 75 >360 Peptide 7 in Example 7 60 120 80 >360 75 >360 Peptide 8 in Example 8 50 150 80 300 80 >360 Peptide 9 in Example 9 55 180 80 300 80 >360 Peptide 10 in Example 10 55 200 80 300 80 >360 Peptide 11 in Example 11 65 300 85 >360 85 >360 Peptide 12 in Example 12 65 300 90 >360 85 >360 Peptide 13 in Example 13 65 300 90 >360 85 >360 Peptide 14 in Example 14 40 100 80 240 85 >360 Peptide 15 in Example 15 40 100 80 240 85 >360 Example 16 Purified peptides 1 to 14 obtained in Examples 1 to 14 were examined for their blood-flow increasing action and compared with those of VIP and PACAP. Wistar male rats each weighing 220 to 250 g were anesthetized by intraperitoneal administration of pentobarbital (25 mg/kg) and fixed on their back. An arterial canula was inserted into the carotid and then connected to a blood-pressure transducer (DX-300, Nippon Koden, Japan) via a doom kit (SCK-512, Nippon Koden) filled with heparin-containing physiological saline (10 U/ml) to measure blood pressure with a strain blood-pressure amplifier (AP-601G, Nippon Koden). Further, a blood-flow measurement probe (FR-030T, Nippon Koden) was attached to the right femoral artery and then connected to an electromagnetic blood-flow meter (MFV-3200, Nippon Koden) to measure blood flow. The dosage of each peptide intraarterially administrated was 100 pmol/kg. Table 2 shows an increase in blood flow in rats given each peptide. Blood Flow (ml/min) PACAP 16.04±5.92 VIP 10.18±3.38 Peptide 1 in Example 1 50.02±42.34 Peptide 2 in Example 2 52.63±39.58 Peptide 3 in Example 3 52.13±37.85 Peptide 4 in Example 4 51.87±37.24 Peptide 5 in Example 5 54.60±41.05 Peptide 6 in Example 6 53.71±46.46 Peptide 7 in Example 7 55.05±46.71 Peptide 8 in Example 8 53.75±37.31 Peptide 9 in Example 9 54.26±35.95 Peptide 10 in Example 10 55.86±34.69 Peptide 11 in Example 11 57.04±38.67 Peptide 12 in Example 12 57.34±41.88 Peptide 13 in Example 13 56.76±36.15 Peptide 14 in Example 14 52.92±32.12 Peptide 15 in Example 15 52.63±31.05 No particular change was observed in the blood pressure of rats given 100 pmol/kg of each of peptides 1 to 14. Example 17 Acute Toxicity Test Peptides 1 to 14 obtained in Examples 1 to 14 were dissolved in physiological saline and administered intravenously into mice at a dosage of 10 mg/kg. The result indicated that every mouse survived. SEQUENCE LISTING SEQ ID NO: 1: LENGTH: 27 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 1: SEQ ID NO: 2: LENGTH: 28 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 2: SEQ ID NO: 3: LENGTH: 29 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 3: SEQ ID NO: 4: LENGTH: 29 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 4: SEQ ID NO: 5: LENGTH: 30 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 5: SEQ ID NO: 6: LENGTH: 30 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 6: SEQ ID NO: 7: LENGTH: 30 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 7: SEQ ID NO: 8: LENGTH: 29 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 8: SEQ ID NO: 9: LENGTH: 30 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 9: SEQ ID NO: 10: LENGTH: 30 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 10: SEQ ID NO: 11: LENGTH: 31 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 11: SEQ ID NO: 12: LENGTH: 31 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 12: SEQ ID NO: 13: LENGTH: 31 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 13: SEQ ID NO: 14: LENGTH: 27 amino acids TYPE: amino acid TOPOLOGY: linear MOLECULE TYPE: peptide SEQUENCE DESCRIPTION: SEQ ID NO: 14:

Claims: A peptide represented by the formula: or or the general formula 1: wherein A is Ala or Gly; B is lie or Val; C is Asn or Ser; D is Thr or Ser; E is Leu or Tyr; each of F, I and J is Lys or Arg, and at least one of F, I and J is Arg; G is Met, Leu or nLeu; K is Asn or Ala; L is Ser or Ala; M is Ile or Val; P is Asn or a chemical bond; Q is Lys, Arg, Lys-Arg, Arg-Arg or a chemical bond; and R is -OH or -NH 2 , or pharmaceutically acceptable salts thereof. The peptide of claim 1 represented by the formula: or or pharmaceutically acceptable salts thereof. A bronchus-expanding agent comprising a peptide of claim 1 or 2, or a pharmaceutically acceptable salt thereof as an active ingredient. A blood-flow-improving agent comprising a peptide of claim 1 or 2 or a pharmaceutically acceptable salt thereof as an active ingredient. A pharmaceutical composition comprising a peptide of claim 1 or 2, or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier. Use of a peptide of claim 1 or 2, or a pharmaceutically acceptable salt thereof for the preparation of a pharmaceutical composition for the treatment of asthma. Use of a peptide of claim 1 or 2, or a pharmaceutically acceptable salt thereof for the preparation of a pharmaceutical composition for the treatment of ulcer, frostbite, bedsore, impotence and in the restoration and growth of hair.

Inventors: KASHIMOTO KAZUHISA, NAGANO YUMIKO, KASHIMOTO, KAZUHISA, NAGANO, YUMIKO, KASHIMOTO, Kazuhisa, Itoham Foods Inc., NAGANO, Yumiko, Itoham Foods Inc.
Applicants: ITOHAM FOODS INC, ITOHAM FOODS INC.
Application year: 1996
Main classification: C07K14
IPC codes: A61P7, A61K38, C07K14