METHOD OF TREATMENT FOR LUNG DISEASES USING ANTISENSE OLIGONUCLEOTIDES

Abstract: A method of treating airway disease in a subject in need of such treatment is disclosed. The method comprises topically administering to the subject an antisense oligonucleotide in an amount effective to treat the ariway disease, where the antisense oligonucleotide is essentially free of adenosine. Pharmaceutical formulations are also disclosed.

Description: METHOD OF TREATMENT FOR LUNG DISEASES USING ANTISENSE OLIGONUCLEOTIDES This invention was made with Government support under grant RO1CA47217-06 from the National Cancer Institute. The Government has certain rights to this invention. Field of the Invention This application concerns a method of administering antisense oligonucleotides essentially free of adenosine as a treatment for lung diseases. Background of the Invention Antisense oligonucleotides have received considerable theoretical consideration as potentially useful pharmacologic agents in human disease. R. Wagner, Nature 372, 333-335 (1994) . However, practical applications of these molecules in actual models of human disease have been elusive. One important consideration in the pharmacologic application of these molecules is route of administration. Most experiments utilizing antisense oligonucleotides in vivo have involved direct application to limited regions of the brain (see C. Wahlestedt, Trends in Pharmacological Sciences 15, 42-46 (1994) ; J. Lai et al. , Neuroreport 5, 1049-1052 (1994) ; K. Standifer et al . , Neuron 12, 805-810 (1994) ; A. Akabayashi et al. , Brain Research 21, 55-61 (1994)) , or to spinal fluid (see e.g. L. Tseng et al . , European J. Pharmacol . 258, Rl-3 (1994) ; R. Raffa et al . , European J. Pharmacol . 258, R5-7 (1994) ; F. Gillardon et al . , European J. Neurosci . 6, 880-884 (1994)) . Such applications have limited clinical utility due to their invasive nature. The systemic administration of antisense oligonucleotides also poses significant problems with respect to pharmacologic application, not the least of which is the difficulty in targeting disease-involved tissues. In contrast, the lung is an excellent potential target for antisense oligonucleotide application since it may be approached noninvasively and in a tissue-specific manner. Additionally, the lung represents an exceptional target for antisense ODN therapeutics ascompared to other in vivo target organs or tissues, possibly because the lung is lined with surfactant which consists primarily of cationic lipids, well known to enhance cellular uptake of ODNs in other systems. However, the technology involved in delivering antisense agents to the lung remains relatively undeveloped, and potential problems related to the application of antisense agents to the lung remain unexplored. Adenosine, a purine which contributes to intermediary metabolism and participates in the regulation of physiological activity, is a recognized neuromodulator. This nucleoside is involved in many local regulatory mechanisms, in particular at synapses in the CNS and at neuroeffector junctions in the periphery. In the CNS adenosine is known to inhibit the release of a variety of neurotransmitters (noradrenaline, serotonin, GABA, acetylcholine, dopamine, glutamate, etc.), to inhibit neurotransmission, depress neuronal firing, induce spinal analgesia, and to possess anxiolytic properties (E.S. Ben-Soreket al. , Archives of Internal Medicine 153, 2701-2702 (1993)) . In the heart, adenosine is known to slow atrioventricular (AV) conduction, suppress pacemaker activity, possess antiarrhythmic effects, modulate autonomic control, and to trigger the synthesis and release of prostaglandins. M.K. Church et al., J " . Allergy & Clinical Immunology 92, 190-194 (1993) . It also possesses potent vasodilatory effects and modulates vascular tone. S.T. Holgate et al . , Annals of the New York Academy of Sciences 629, 227-236 (1991) . As a therapeutic agent, adenosine has achieved considerable recent success as an antiarryhthmic agent in the treatment of supraventricular tachycardia. See C.G. DeGroff and M.J. Silka, Journal of Pediatrics 125, 822- 823 (1994); I. Drake et al. , Human and Exp. Toxicol . 13, 263-265 (1994) . However, many adverse effects of adenosine treatment have been reported in the literature. See, e.g., A. Aggarwal, et al. , Anesthesiology 79, 1132- 1135 (1993) ; K.K. Burkhart, American J. Emergency Med. 11, 249-250 (1993) ; S.K. Srinivasan and P.J. Iversen, J. Clin . Lab. Analysis 9, 129- 137 (1995) ; C.A. Stein et al . , Pharmacology & Therapeutics 52, 365-384 (1991) ; B.B. Fredholm et al . , Pharmacological Reviews 46, 143-156 (1994) ; H. Saito, et al. , Blood 66, 1233-1240 (1985) . In particular, asthmatic individuals show an extreme sensitivity to adenosine and adenosine monophosphate. See, J.H. Butterfield et al. , Leukemia Res . 12, 345-355 (1988) ; CLONETICS : Normal Human Cell Systems Manual (1995); R.W. Wagner, Nature 372, 333-335 (1994) . Serious, near-fatal induction of bronchospasm has occurred in asthmatic individuals administered adenosine for supraventricular tachycardia. See, S. Tabor, in: Current Protocols in Mol ecular Biology, Vol . 1 , Section 3.10.2 (John Wiley & Sons, 1987) ; J.H. Weiss, Id . , at Section 6.2.2. Similarly, asthmatic rabbits produced using the dust mite allergic rabbit model of human asthma also were shown to respond to aerosolized adenosine with marked bronchoconstriction, while non asthmatic rabbits showed no response. S. Ali et al. , Agents Actions 37, 165-176 (1992) . Recent work using this model system has suggested that adenosine-induced bronchoconstriction and bronchial hyperresponsiveness in asthma are mediated primarily through the stimulation of adenosine receptors. S. Ali et al., J " . Pharmacol . Exp . Ther. 268, 1328-1334 (1994) ; S. Ali et al., Am. J. Physiol 266, L271-277 (1994) . Accordingly, adenosine is contraindicated in the lungs of asthmatics (who represent 10% of the adult and 15% of the pediatric population in the United States) . Since antisense ODNs are typically composed of all four base pairs, adenine, guanine, cytosine and thymidine, their breakdown products will produce free deoxyadenosine monophosphate in these hyperresponsive airways. Deoxyadenosine monophosphate differs from adenosine monophosphate only by the loss of an oxygen atom on the 3' carbon of the sugar moiety. Summary of the Invention A first aspect of the present invention is a method of treating airway disease in a subject in need of such treatment. The method comprises administering an antisense oligonucleotide essentially free of adenosine to the lungs of the subject in an amount effective to treat the airway disease. A second aspect of the present invention is a pharmaceutical composition, comprising, together in a pharmaceutically acceptable carrier, an antisense oligonucleotide essentially free of adenosine in an amount effective to treat an airway disease. A third aspect of the present invention is the use of an antisense oligonucleotide essentially free of adenosine as given above for the preparation of a medicament for treating airway disease in a subject in need of such treatment. Brief Description of the Drawings Figures 1-4 demonstrate that antisense oligonucleotides can be utilized as effective agents in the treatment or prevention of airway diseases. Figure 1 illustrates the effects of adenosine receptor antisense oligonucleotides and mismatch control antisense oligonucleotides on the dynamic compliance of the bronchial airway in a rabbit model. Figure 2 illustrates the specificity of A τ adenosine receptor antisense oligonucleotides as indicated by the A λ and A 2 adenosine receptor number present in A 1 adenosine receptor antisense oligonucleotide-treated airway tissue. Figure 3 is a graphical representation illustrating that aerosolized deoxyadenosine monophosphate is a potent bronchoconstrictor in asthmatic pathways of allergic rabbits. Further, the figure shows that the effect of deoxyadenosine monophosphate is equipotent to that observed for adenosine monphosphate. Figure 4 is a graphical representation illustrating that bronchoconstrictor effects occur with aerosolized phosphorothioate oligodeoxynucleotides containing adenosine, but not with oligodeoxynucleotides that are free of adenosine. Detailed Description of the Invention Nucleotide sequences are presented herein by single strand only, in the 5' to 3' direction, from left to right . Nucleotides and amino acids are represented herein in the manner recommended by the IUPAC-IUB Biochemical Nomenclature Commission, or (for amino acids) by three letter code, in accordance with 37 CFR §1.822 and established usage. See, e . g. , Patentin User Manual , 99-102 (Nov. 1990) (U.S. Patent and Trademark Office, Office of the Assistant Commissioner for Patents, Washington, D.C. 20231); U.S. Patent No. 4,871,670 to Hudson et al . at Col. 3 - lines 20-43 (applicants specifically intend that the disclosure of this and all other patent references cited herein be incorporated herein by reference) . The method of the present invention may be used to treat airway disease in a subject for any reason, with the intention that adenosine content of antisense compounds be eliminated or reduced so as to prevent its liberation upon antisense degredation. Such liberation may cause serious, even life-threatening, bronchoconstriction in patients with hyperreactive airways. Examples of airway diseases that may be treated by the method of the present invention include cystic fibrosis, asthma, chronic obstructive pulmonary disease, bronchitis, and other airway diseases characterized by an inflammatory response. Antisense oligonucleotides to the A x and A 3 receptors are shown to be effective in the downregulation of A x or A 3 in the cell. One novel feature of this treatment, as compared to traditional treatments for adenosine-induced bronchoconstriction, is that administration is direct to the lungs. Additionally, a receptor protein itself is reduced in amount, rather than merely interacting with a drug, and toxicity is reduced. Other proteins that may be targeted with antisense agents for the treatment of lung conditions include, but are not limited to: human A2a adenosine receptor, human A2b adenosine receptor, human IgE receptor β , human Fc- epsilon receptor CD23 antigen, human histidine decarboxylase, human beta tryptase, human tryptase-I, human prostaglandin D synthase, human cyclooxygenase-2, human eosinophil cationic protein, human eosinophil derived neurotoxin, human eosinophil peroxidase, human intercellular adhesion molecule-1 (ICAM-1) , human vascular cell adhesion molecule 1 (VCAM-1) , human endothelial leukocyte adhesion molecule (ELAM-1) , human P selectin, human endothelial monocyte activating factor, human IL-3, human IL-4, human IL-5, human IL-6, human IL- 8, human monocyte-derived neutrophil chemotactic factor, human neutrophil elastase, human neutrophil oxidase factor, human cathepsin G, human defensin 1, human defensin 3, human macrophage inflammatory protein-1- alpha, human muscarinic acetylcholine receptor HM1, human muscarinic acetylcholine receptor HM3, human fibronectin, human GM-CSF, human tumor necrosis factor , human leukotriene C4 synthase, human major basic protein, and human endothelin 1. In these latter targets, and in target genes in general, it is particularly imperative to eliminate or reduce the adenosine content of the corresponding antisense oligonucleotide to prevent their breakdown products from liberating adenosine. As used herein, the term "treat" or "treating" a lung disease refers to a treatment which decreases the likelihood that the subject administered such treatment will manifest symptoms of the lung disease. The term "downregulate" refers to inducing a decrease in production, secretion or availability (and thus a decrease in concentration) of the targeted intracellular protein. The present invention is concerned primarily with the treatment of human subjects but may also be employed for the treatment of other mammalian subjects, such as dogs and cats, for veterinary purposes. Targeted proteins are preferably mammalian and more preferably of the same species as the subject being treated. In general, "antisense" refers to the use of small, synthetic oligonucleotides, resembling single- stranded DNA, to inhibit gene expression by inhibiting the function of the target messenger RNA (mRNA) . Milligan, J.F. et al. , J. Med. Chem. 36(14) , 1923-1937 (1993) . In the present invention, inhibition of gene expression of the A x or A 3 adenosine receptor is desired. Gene expression is inhibited through hybridization to coding (sense) sequences in a specific messenger RNA (mRNA) target by hydrogen bonding according to Watson- Crick base pairing rules. The mechanism of antisense inhibition is that the exogenously applied oligonucleotides decrease the mRNA or protein levels of the target gene or cause changes in the growth characteristics or shapes of the cells. Id. See also Helene, C. and Toulme, J. , Biochim. Biophys . Acta 1049, 99-125 (1990) ; Cohen, J.S., Ed., Oligodeoxynucleotides as Antisense Inhibi tors of Gene Expression; CRC Press:Boca Raton, FL (1987) . As used herein, "antisense oligonucleotide" is defined as a short sequence of synthetic nucleotides that (1) hybridizes to any coding sequence in an mRNA which codes for the targeted protein, according to hybridization conditions described below, and (2) upon hybridization causes a decrease in gene expression of the targeted protein. The mRNA sequence of the A x or A 3 adenosine receptor is derived from the DNA base sequence of the gene expressing either the A x or A 3 adenosine receptor. The sequence of the genomic human A x adenosine receptor is known and is disclosed in U.S. Patent No. 5,320,963 to G. Stiles et al. The A 3 adenosine receptor has been cloned, sequenced and expressed in rat (see F. Zhou et al . , Proc. Nat 'l Acad. Sci . USA 89:7432 (1992)) and human (see M.A. Jacobson et al . , U.K. Patent Application No. 9304582.1 (1993)) . Thus, antisense oligonucleotides that downregulate the production of the A or A 3 adenosine receptor may be produced in accordance with standard techniques. One aspect of this invention is an antisense oligonucleotide having a sequence capable of binding specifically with any sequence of an mRNA molecule which encodes an airway disease-associated protein so as to prevent translation of the mRNA molecule. Chemical analogs of oligonucleotides (e.g., oligonucleotides in which the phosphodiester bonds have been modified, e.g., to the methylphosphonate, the phosphotriester, the phosphorothioate , the phosphorodithioate, or the phosphoramidate, so as to render the oligonucleotide more stable in vivo) are also an aspect of the present invention. The naturally occurring phosphodiester linkages in oligonucleotides are susceptible to degradation by endogenously occurring cellular nucleases, while many analogous linkages are highly resistant to nuclease degradation. See Milligan et al., and Cohen, J.S., supra . Protection from degradation can be achieved by use of a "3' -end cap" strategy by which nuclease-resistant linkages are substituted for phosphodiester linkages at the 3' end of the oligonucleotide. See Tidd, D.M. and Warenius, H.M., Br. J. Cancer 60, 343-350 (1989) ; Shaw, J.P. et al. , Nucleic Acids Res . 19, 747-750 (1991) . Phosphoramidates, phosphorothioates, and methylphosphonate linkages all function adequately in this manner. More extensive modification of the phosphodiester backbone has been shown to impart stability and may allow for enhanced affinity and increased cellular permeation of oligonucleotides. See Milligan, et al . , supra . Many different chemical strategies have been employed to replace the entire phosphodiester backbone with novel linkages. Id. Backbone analogues include phosphorothioate, phosphorodithioate, methylphosphonate, phosphoramidate, boranophosphate, phosphotriester, formacetal, 3' -thioformacetal, 5' -thioformacetal, 5'- thioether, carbonate, 5' -N-carbamate, sulfate, sulfonate, sulfamate, sulfonamide, sulfone, sulfite, sulfoxide, sulfide, hydroxylamine, methylene (methylimino) (MMI) or methyleneoxy (methylimino) (MOMI) linkages. Phosphorothioate and methylphosphonate-modified oligonucleotides are particularly preferred due to their availability through automated oligonucleotide synthesis. Id. Where appropriate, the antisense oligonucleotides may be administered in the form of their pharmaceutically acceptable salts. Antisense oligonucleotides may be of any suitable length (e.g., from about 10 to 60 nucleotides in length) , depending on the particular target being bound and the mode of delivery thereof. Preferably the antisense oligonucleotide is directed to an mRNA region containing a junction between intron and exon. Where the antisense oligonucleotide is directed to an intron/exon junction,- it may either entirely overlie the junction or may be sufficiently close to the junction to inhibit splicing out of the intervening exon during processing of precursor mRNA to mature mRNA (e.g., with the 3' or 5' terminus of the antisense oligonucleotide being is positioned within about, for example, 10, 5, 3, or 2 nucleotides of the intron/exon junction) . When practicing the present invention, the antisense nucleotides administered may be related in origin to the species to which it is administered. When treating humans, human antisense may be used if desired. Pharmaceutical compositions comprising an antisense oligonucleotide as given above effective to reduce expression of an A_ or A 3 adenosine receptor by passing through a cell membrane and binding specifically with mRNA encoding an A x or A 3 adenosine receptor in the cell so as to prevent its translation are another aspect of the present invention. Such compositions are provided in a suitable pharmaceutically acceptable carrier (e.g., sterile pyrogen-free saline solution) . The antisense oligonucleotides may be formulated with a hydrophobic carrier capable of passing through a cell membrane (e.g., in a liposome, with the liposomes carried in a pharmaceutically acceptable aqueous carrier) . The oligonucleotides may also be coupled to a substance which inactivates mRNA, such as a ribozyme. Such oligonucleotides may be administered to a subject to inhibit the activation of A x or A 3 adenosine receptors, which subject is in need of such treatment for any of the reasons discussed herein.. Furthermore, the pharmaceutical formulation may also contain chimeric molecules comprising antisense oligonucleotides attached to molecules which are known to be internalized by cells. These oligonucleotide conjugates utilize cellular uptake pathways to increase cellular concentrations of oligonucleotides. Examples of macromolecules used in this manner include transferrin, asialoglycoprotein (bound to oligonucleotides via polylysine) and streptavidin. In the pharmaceutical formulation the antisense compound may be contained within a lipid particle or vesicle, such as a liposome or microcrystal . The particles may be of any suitable structure, such ' as unilamellar or plurilamellar, so long as the antisense oligonucleotide is contained therein. Positively charged lipids such as N- [1- (2, 3-dioleoyloxi)propyl] -N,N,N- trimethyl-ammoniumethylsulfate, or "DOTAP, " are particularly preferred for such particles and vesicles. The preparation of such lipid particles is well known. See, e . g. , U.S. Patent Nos. 4,880,635 to Janoff et al. ; 4,906,477 to Kurono et al. ; 4,911,928 to Wallach; 4,917,951 to Wallach; 4,920,016 to Allen et al . ; , 921, 757 to Wheatley et al. ; etc. Subjects may be administered the active composition by any means which transports the antisense nucleotide composition to the lung. The antisense compounds disclosed herein may be administered to the lungs of a patient by any suitable means, but are preferably administered by generating an aerosol comprised of respirable particles, the respirable particles comprised of the antisense compound, which particles the subject inhales. The respirable particles may be liquid or solid. The particles may optionally contain other therapeutic ingredients. Particles comprised of antisense compound for practicing the present invention should include particles of respirable size: that is, particles of a size sufficiently small to pass through the mouth and larynx upon inhalation and into the bronchi and alveoli of the lungs. In general, particles ranging from about .5 to 10 microns in size are respirable. Particles of non- respirable size which are included in the aerosol tend to deposit in the throat and be swallowed, and the quantity of non-respirable particles in the aerosol is preferably minimized. For nasal administration, a particle size in the range of 10-500 μm is preferred to ensure retention in the nasal cavity. Liquid pharmaceutical compositions of active compound for producing an aerosol can be prepared by combining the antisense compound with a suitable vehicle, such as sterile pyrogen free water. Other therapeutic compounds may optionally be included. Solid particulate compositions containing respirable dry particles of micronized antisense compound may be prepared by grinding dry antisense compound with a mortar and pestle, and then passing the micronized composition through a 400 mesh screen to break up or separate out large agglomerates. A solid particulate composition comprised of the antisense compound may optionally contain a dispersant which serves to facilitate the formation of an aerosol. A suitable dispersant is lactose, which may be blended with the antisense compound in any suitable ratio (e.g., a 1 to 1 ratio by weight) . Again, other therapeutic compounds may also be included. The dosage of the antisense compound administered will depend upon the disease being treated, the condition of the subject, the particular formulation, the route of administration, the timing of administration to a subject, etc. In general, intracellular concentrations of the oligonucleotide of from .05 to 50 μM, or more particularly .2 to 5 μM, are desired. For administration to a subject such as a human, a dosage of from about .01, .1, or 1 mg/Kg up to 50, 100, or 150 mg/Kg or more is typically employed. Depending on the solubility of the particular formulation of active compound administered, the daily dose may be divided among one or several unit dose administrations. Administration of the antisense compounds may be carried out therapeutically (i.e., as a rescue treatment) or prophylactically. ' Aerosols of liquid particles comprising the antisense compound may be produced by any suitable means, such as with a nebulizer. See, e . g. , U.S. Patent No. 4,501,729. Nebulizers are commercially available devices which transform solutions or suspensions of the active ingredient into a therapeutic aerosol mist either by means of acceleration of a compressed gas, typically air or oxygen, through a narrow venturi orifice or by means of ultrasonic agitation. Suitable formulations for use in nebulizers consist of the active ingredient in a liquid carrier, the active ingredient comprising up to 40% w/w of the formulation, but preferably less than 20% w/w. the carrier is typically water or a dilute aqueous alcoholic solution, preferably made isotonic with body fluids by the addition of, for example, sodium chloride. Optional additives include preservatives if the formulation is not prepared sterile, for example, methyl hydroxybenzoate, antioxidants, flavoring agents, volatile oils, buffering agents and surfactants. Aerosols of solid particles comprising the active compound may likewise be produced with any solid particulate medicament aerosol generator. Aerosol generators for administering solid particulate medicaments to a subject produce particles which are respirable, as explained above, and generate a volume of aerosol containing a predetermined metered dose of a medicament at a rate suitable for human administration. One illustrative type of solid particulate aerosol generator is an insufflator. Suitable formulations for administration by insufflation include finely comminuted powders which may be delivered by means of an insufflator or taken into the nasal cavity in the manner of a snuff. In the insufflator, the powder (e.g., a metered dose thereof effective to carry out the treatments described herein) is contained in capsules or cartridges, typically made of gelatin or plastic, which are either pierced or opened in si tu and the powder delivered by air drawn through the device upon inhalation or by means of a manually-operated pump. The powder employed in the insufflator consists either solely of the active ingredient or of a powder blend comprising the active ingredient, a suitable powder diluent, such as lactose, and an optional surfactant. The active ingredient typically comprises from 0.1 to 100 w/w of the formulation. A second type of illustrative aerosol generator comprises a metered dose inhaler. Metered dose inhalers are pressurized aerosol dispensers, typically containing a suspension or solution formulation of the active ingredient in a liquified propellant. During use these devices discharge the formulation through a valve adapted to deliver a metered volume, typically from 10 to 150 μl, to produce a fine particle spray containing the active ingredient. Suitable propellants include certain chlorofluorocarbon compounds, for example, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane and mixtures thereof. The formulation may additionally contain one or more co- solvents, for example, ethanol, surfactants, such as oleic acid or sorbitan trioleate, antioxidants and suitable flavoring agents. The aerosol, whether formed from solid or liquid particles, may be produced by the aerosol generator at a rate of from about 10 to 150 liters per minute, more preferably from about 30 to 150 liters per minute, and most preferably about 60 liters per minute. Aerosols containing greater amounts of medicament may be administered more rapidly. The following examples are provided to illustrate the present invention, and should not be construed as limiting thereon. In these examples, μM means micromolar, mL means milliliters, μm means micrometers, mm means millimeters, cm means centimeters, °C means degrees Celsius, μg means micrograms, mg means milligrams, g means grams, kg means kilograms, M means molar, and h means hours. EXAMPLE 1 Design and synthesis of antisense oligonucleotides The design of antisense oligonucleotides against the A 1 and A 3 adenosine receptors may require the solution of the complex secondary structure of the target A x receptor mRNA and the target A 3 receptor mRNA. After generating this structure, antisense nucleotides are designed which target regions of mRNA which might be construed to confer functional activity or stability to the mRNA and which optimally may overlap the initiation codon. Other target sites are readily usable. As a demonstration of specificity of the antisense effect, other oligonucleotides not totally complementary to the target mRNA, but containing identical nucleotide compositions on a w/w basis, are included as controls in antisense experiments. Adenosine A receptor mRNA secondary structure was analyzed and used as described above to design a phosphorothioate antisense oligonucleotide. The antisense oligonucleotide which was synthesized was designated HAdAlAS and had the following sequence: 5' -GAT GGA GGG CGG CAT GGC GGG-3' (SEQ ID NO:l) As a control, a mismatched phosphorothioate antisense nucleotide designated HAdAlMM was synthesized with the following sequence: 5'-GTA GCA GGC GGG GAT GGG GGC-3' (SEQ ID NO:2) Each oligonucleotide had identical base content and general sequence structure. Homology searches in GENBANK (release 85.0) and EMBL (release 40.0) indicated that the antisense oligonucleotide was specific for the human and rabbit adenosine A 1 receptor genes, and that the mismatched control was not a candidate for hybridization with any known gene sequence. Adenosine A 3 receptor mRNA secondary structure was similarly analyzed and used as described above to design two phosphorothioate antisense oligonucleotides. The first antisense oligonucleotide (HAdA3ASl) synthesized had the following sequence: 5'-GTT GTT GGG CAT CTT GCC-3' (SEQ ID NO:3) As a control, a mismatched phosphorothioate antisense oligonucleotide (HAdA3MMl) was synthesized, having the following sequence: 5'-GTA CTT GCG GAT CTA GGC-3' (SEQ ID NO:4) A second phosphorothioate antisense oligonucleotide (HAdA3AS2) was also designed and synthesized, having the following sequence: 5'-GTG GGC CTA GCT CTC GCC-3' (SEQ ID NO:5) Its control oligonucleotide (HAdA3MM2) had the sequence: 5'-GTC GGG GTA CCT GTC GGC-3' (SEQ ID NO:6) Phosphorothioate oligonucleotides were synthesized on an Applied Biosystems Model 396 Oligonucleotide Synthesizer, and purified using NENSORB chromatography (DuPont, MD) . EXAMPLE 2 Testing of Al-Adenosine Receptor Antisense Oligonucleotides in vi tro The antisense oligonucleotide against the human A- L receptor (SEQ ID NO:l) described above was tested for efficacy • in an in vi tro model utilizing lung adenocarcinoma cells HTB-54. HTB-54 lung adenocarcinoma cells were demonstrated to express the A 1 adenosine receptor using standard northern blotting procedures and receptor probes designed and synthesized in the laboratory. HTB-54 human lung adenocarcinoma cells (106/100 mm tissue culture dish) were exposed to 5.0 μM HAdAlAS or HAdAIMM for 24 hours, with a fresh change of media and oligonucleotides after 12 hours of incubation. Following 24 hour exposure to the oligonucleotides, cells were harvested and their RNA extracted by standard procedures. A 21-mer probe corresponding to the region of mRNA targeted by the antisense (and therefore having the same sequence as the antisense, but not phosphorothioated) was synthesized and used to probe northern blots of RNA prepared from HAdAlAS-treated, HAdAIMM-treated and non- treated HTB-54 cells. These blots showed clearly that HAdAlAS but not HAdAIMM effectively reduced human adenosine receptor mRNA by >50%. This result showed that HAdAlAS is a good candidate for an anti-asthma drug since it depletes intracellular mRNA for the adenosine A x receptor, which is involved in asthma. EXAMPLE 3 Efficacy of A τ -Adenosine Receptor Antisense Oligonucleotides in vivo A fortuitous homology between the rabbit and human DNA sequences within the adenosine A x gene overlapping the initiation codon permitted the use of the phosphorothioate antisense oligonucleotides initially designed for use against the human adenosine A x receptor in a rabbit model. Neonatal New Zealand white Pasteurella-free rabbits were immunized intraperitoneally within 24 hours of birth with 312 antigen units/ L house dustmite {D. farinae) extract (Berkeley Biologicals, Berkeley, CA) , mixed with 10% kaolin. Immunizations were repeated weekly for the first month and then biweekly for the next 2 months. At 3-4 months of age, eight sensitized rabbits were anesthetized and relaxed with a mixture of ketamine hydrochloride (44 mg/kg) and acepromazine maleate (0.4 mg/kg) administered intramuscularly. The rabbits were then laid supine in a comfortable position on a small molded, padded animal board and intubated with a 4.0-mm intratracheal tube (Mallinkrodt, Inc., Glens Falls, NY) . A polyethylene catheter of external diameter 2.4 mm with an attached latex balloon was passed into the esophagus and maintained at the same distance (approximately 16 cm) from the mouth throughout the experiments. The intratracheal tube was attached to a heated Fleisch pneumotachograph (size 00; DOM Medical, Richmond, VA) , and flow was measured using a Validyne differential pressure transducer (Model DP-45161927; Validyne Engineering Corp., Northridge, CA) driven by a Gould carrier amplifier (Model 11-4113; Gould Electronic, Cleveland, OH) . The esophageal balloon was attached to one side of the differential pressure transducer, and the outflow of the intratracheal tube was connected to the opposite side of the pressure transducer to allow recording of transpulmonary pressure. Flow was integrated to give a continuous tidal volume, and measurements of total lung resistance (RL) and dynamic compliance (Cdyn) were calculated at isovolumetric and flow zero points, respectively, using an automated respiratory analyzer (Model 6; Buxco, Sharon, CT) . Animals were randomized and on Day 1 pretreatment values for PC50 were obtained for aerosolized adenosine. Antisense (HAdAlAS) or mismatched control (HAdAIMM) oligonucleotides were dissolved in sterile physiological saline at a concentration of 5000 ug (5 mg) per 1.0 ml. Animals were subsequently administered the aerosolized antisense or mismatch oligonucleotide via the intratracheal tube (approximately 5000 μg in a volume of 1.0 ml), twice daily for two days. Aerosols of either saline, adenosine, or antisense or mismatch oligonucleotides were generated by an ultrasonic nebulizer (DeVilbiliss, Somerset, PA) , producing aerosol droplets 80% of which were smaller than 5 μm in diameter. In the first arm of the experiment, four randomly selected allergic rabbits were administered antisense oligonucleotide and four the mismatched control oligonucleotide. On the morning of the third day, PC50 values (the concentration of aerosolized adenosine in mg/ml required to reduce the dynamic compliance of the bronchial airway 50% from the baseline value) were obtained and compared to PC50 values obtained for these animals prior to exposure to oligonucleotide. Following a 1 week interval, animals were crossed over, with those previously administered mismatch control oligonucleotide now administered antisense oligonucleotide, and those previously treated with antisense oligonucleotide now administered mismatch control oligonucleotide. Treatment methods and measurements were identical to those employed in the first arm of the experiment. It should be noted that in six of the eight animals treated with antisense oligonucleotide, adenosine-induced bronchoconstriction could not be obtained up to the limit of solubility of adenosine, 20 mg/ml. For the purpose of calculation, PC50 values for these animals were set at 20 mg/ml. The values given therefore represent a minimum figure for antisense effectiveness. Actual effectiveness was higher. The results of this experiment are illustrated in both Figure 1 and Table 1. TABLE 1. EFFECTS OF ADENOSINE A, RECEPTOR ANTISENSE OLIGONUCLEOTIDE UPON PC50 VALUES IN ASTHMATIC RABBITS. Mismatch Control A, receptor Antisense oligonucleotide Pre Post Pre Post oligonucleotide oligonucleotide oligonucleotide oligonucleotide 3.56 ± 1.02 5.16 ± 1.93 2.36 ± 0.68 >19.5 ± 0.34** Results are presented as the mean (N = 8) ± SEM. Significance was determined by repeated- measures analysis of variance (ANOVA), and Tu ey's protected t test. ** Significantly different from all other groups, P < 0.01. In both arms of the experiment, animals receiving the antisense oligonucleotide showed an order of magnitude increase in the dose of aerosolized adenosine required to reduce dynamic compliance of the lung by 50%. No effect of the mismatched control oligonucleotide upon PC50 values was observed. No toxicity was observed in any animal receiving either antisense or control inhaled oligonucleotide. These results show clearly that the lung has exceptional potential as a target for antisense oligonucleotide-based therapeutic intervention in lung disease. They further show, in a model system which closely resembles human asthma, that downregulation of the adenosine A receptor largely eliminates adenosine- induced bronchoconstriction in asthmatic airways. Bronchial hyperresponsiveness in the allergic rabbit model of human asthma is an excellent endpoint for antisense intervention since the tissues involved in this response lie near to the point of contact with aerosolized oligonucleotides, and the model closely simulates an important human disease. EXAMPLE 4 Specificity of A-,-adenosine receptor Antisense oligonucleotide At the conclusion of the crossover experiment of Example 3, airway muscle from all rabbits was quantitatively analyzed for adenosine A x receptor number. As a control for the specificity of the antisense oligonucleotide, adenosine A 2 receptors, which should not have been affected, were also quantified. Airway smooth muscle tissue was dissected from each rabbit and a membrane fraction prepared according to described methods (J. Kleinstein and H. Glossmann, Naunyn-Schmiedeberg' s Arch . Pharmacol . 305, 191-200 (1978) , with slight modifications. Crude plasma membrane preparations were stored at - 70°C until the time of assay. Protein content was determined by the method of Bradford (M. Bradford, Anal. Biochem. 72, 240-254 (1976) ) . Frozen plasma membranes were thawed at room temperature and were incubated with 0.2 U/ml adenosine deaminase for 30 minutes at 37°C to remove endogenous adenosine. The binding of [ 3 H]DPCPX (A x receptor- specific) or [ 3 H] CGS-21680 (A 2 receptor-specific) was measured as previously described. S. Ali et al . , J. Pharmacol . Exp. Ther. 268, 1328-1334 (1994) ; S. Ali et al., Am. J. Physiol 266, L271-277 (1994) . As illustrated in both Figure 2 and Table 2, animals treated with adenosine A 1 antisense oligonucleotide in the crossover experiment had a nearly 75% decrease in A x receptor number compared to controls, as assayed by specific binding of the A x -specific antagonist DPCPX. There was no change in adenosine A 2 receptor number, as assayed by specific binding of the A 2 receptor-specific agonist 2- [p- (2-carboxyethyl ) - phenethylamino] -5' - (N-ethylcarboxamido) adenosine (CGS- 21680) . TABLE 2. SPECIFICITY OF ACTION OF ADENOSINE A, RECEPTOR ANTISENSE OLIGONUCLEOTIDE. Mismatch Control A, Antisense oligonucleotide olig ;onucleotide A,-Specific Binding 1105 ± 48** 293 ± 18 A 2 -Specific Binding 302 ± 22 442 ± 171 Results are presented as the mean (N = 8) ± SEM. Significance was determined by repeated- measures analysis of variance (ANOVA), and Tukey's protected t test. ** Significantly different from mismatch control, P < 0.01. The above demonstrates the effectiveness of antisense oligonucleotides in treating airway diseases. Since the antisense oligonucleotides described above eliminate the receptor systems responsible for adenosine-mediated bronchoconstriction, it may be less imperative to eliminate adenosine from them. However, it would be preferable to eliminate adenosine from even these oligonucleotides. Examples of such adenosine-free oligonucleotides are provided below in Example 5. EXAMPLE 5 The method of the present invention is also practiced with the following antisense oligonucleotides targeted to their corresponding proteins, in essentially the same manner as given above, for the treatment of various conditions in the lungs. Described below is a series of antisense oligonucleotides targetting the mRNA of proteins involved in inflammation. Adenosine has been eliminated from their nucleotide content to prevent its liberation during degradation. In the following, the first sequence provided after the name of the targeted inflammation-involved protein is the antisense sequence that targets the initiation codon, wherein the naturally-occurring adenosine is substituted by one of the following: (1) a universal base that is not adenosine; (2) a adenosine analog that lacks the ability to bind to the adenosine Al and/or A3 receptors; or (3) a "spacer." Any one of these three is represented in the sequence as the letter "B, " recognized by the IUPAC-IUB Nomenclature Commission as "not-A." See Patentin User Manual , p.99 (November 1990) . Listed following the antisense sequence targeted against the initation codon are additional antisense oligonucleotide sequences directed against other portions of the mRNA of the targeted protein. These additional sequences are the "des-adenosine antisense sequences," in that they do not contain adenosine within the sequence. Fragments of the following sequences that are at least ten, and more preferably at least twelve, nucleotides in length are also an aspect of the presnet invention and are useful in carrying out the present invention. Fragments set forth below that span multiple lines of test indicate "5'-" at the beginning thereof, and "-3'" at the end thereof. Human Al adenosine receptor: 5' -GGC GGC CTG GBB BGC TGB GBT GGB GGG CGG CBT GGC GGG CBC BGG CTG GGC-3' des-adenosine antisense sequences: TTT TCC TTC CTT TGT CTC TCT TC GCT CCC GGC TGC CTG CTC GGC CGT GCG GCT CTG TCG CTC CCG GT CCG CCG CCC TCC GGG GGG TC TGC TGC CGT TGG CTG CCC CTT CTG CGG GTC GCC GG TGC TGG GCT TGT GGC GGC CTC TCT TCT GGG CCT GGT CCC TCC GT GGT GGC TCC TCT GC GCT TGG TCC TGG GGC TGC TGC TCT CCT CTC CTT Human A2a adenosine receptor: GTBCBCCGBGGBGCCCBTGBTGGGCBTGCCBCBGBCGBCBGGC des-adenosine antisense sequences : HSA2ARECAS1 TGC TTT TCT TTT CTG GGC CTC (SEQ ID NO: 7) HSA2ARECAS2 TGT GGT CTG TTT TTT TCT G HSA2ARECAS3 GCC CTG CTG GGG CGC TCT CC HSA2ARECAS4 GCC GCC CGC CTG GCT CCC HSA2ARECAS5 GGB GCC CBT GBT GGG CBT GCC HSA2ARECAS6 GTG GTT CTT GCC CTC CTT TGG CTG HSA2ARECAS7 CCG TGC CCG CTC CCC GGC HSA2ARECAS8 CTC CTG GCG GGT GGC CGT TG HSA2ARECAS9 GGC CCG TGT TCC CCT GGG HSA2ARECAS1 GCC TGG GGC TCC CTT CTC TC HSA2ARECAS1 GCC CTT CTT GCT GGG CCT C HSA2ARECAS1 TGC TGC TGC TGG TGC TGT GGC CCCC Human A2b adenosine receptor: 5' -BCBGCGCGTCCTGTGTCTCCBGCBGCBTGGCC GGGCCBGCTGGGCCCC-3' des-adenosine antisense sequences : HSA2BRECAS1: 5' -GGC GCC GTG CCG CGT CTT GGT GGC GGC GG-3' (SEQ ID NO:8) HSA2BRECAS2: 5' -GTT CGC GCC CGC GCG GGG CCC CTC CGG TCC-3' HSA2BRECAS3: 5' -TTG GCC CGC GCG CCC GCC CGT CTC GGG CTG GGC GG-3' HSA2BRECAS4: CGG GTC GGG GCC CCC CGC GGC C HSA2BRECAS5: 5' -GCC TCG GGG CTG GGG CGC TGG TGG CCG GG-3' HSA2BRECAS6 CCG CGC CTC CGC CTG CCG CTT CTG HSA2BRECAS7 GCT GGG CCC CGG GCG CCC CCT HSA2BRECAS8 CCC CTC TTG CTC GGG TCC CCG TG Human A3 adenosine receptor 5'-BCB GBG CBG TGC TGT TGT TGG GCB TCT TGC CTT CCC BGG G-3' des-adenosine antisense oligonucleotides : CCC TTT TCT GGT GGG GTG GTG CTG TTG TTG GGC TTT CTT CTG TTC CC Human IgE receptor β : 5' -BTTTGCTCTCCTBTTBCTTTCTGTGTCCBTTTTTT CBTTBBCCGBGCTGT-3' des-adenosine antisense sequences : HUMIgEjSrASl: TTT CCC CTG GGT CTT CC (SEQ ID NO:9) HUMIgE(8rAS2 : CTC CTG CTC TTT TTT C Human Fc-epsilon receptor CD23 antigen (IgE receptor) 5' -TCTCTGBBTBTTGBCCTTCCTCCBTGGCGGTCCTGCTT GGBTTCTCCCGB-3' des-adenosine antisense sequences : HUMIgErCD23ASl: GCC TGT GTC TGT CCT CCT (SEQ ID NO:10 ) HUMIgErCD23AS2 GCT TCG TTC CTC TCG TTC HUMIgErCD23AS3 CTG CTT GGT GCC CTT GCC G HUMIgErCD23AS4 GTC CTG CTC CTC CGG GCT GTG G HUMIgErCD23AS5 5' -GTC GTG GCC CTG GCT CCG GCTGGT GGG CTC CCC TGG-3' HUMIgErCD23AS6 CCT TCG CTG GCT GGC GGC GTG C HUMIgErCD23AS7 GGG TCT TGC TCT GGG CCT GGC TGT HUMIgErCD23AS8 GGC CGT GGT TGG GGG TCT TC HUMIgErCD23AS9 GCT GCC TCC GTT TGG GTG GC Human IgE receptor, α subunit: 5' -BCBGTBGBGTBGGGGBTTCCBTGGCBGGBGCCBTC TTCTTCBTGGBCTCC-3' and 5' -TTC BBG GBG BCC TTB GGT TTC TGB GGG BCT GCT BBC BCG CCB TCT GGB GC-3' des-adenosine antisense sequences : HUMIgErαASl: GCCTTTCCTGGTTCTCTT (SEQ ID NO:11) GTT GTT TTT GGG GTT TGG CTT Human IgE receptor, Fc epsilon R: 5'-GBT CTC TGB BTB TTGB CCT TCC BTG GCG GTC CTG CTT GGB-3' des-adenosine antisense sequences : HSJGEBFRAS1: GCC TGT GTC TGT CCT CCT (SEQ ID NO:12) HSJGEBFRAS2 GCT TCG TTC CTC TCG TTC HSJGEBFRAS3 CTG CTT GGT GCC CTT GCC G HSJGEBFRAS4 GTC CTG CTC CTC CGG GCT GTG G HSJGEBFRAS5 5' -GTC CTC GCC CTG GCT CCG GCT GGT GGG CTC CCC TGG-3' HSJGEBFRAS6 CCT TCG CTG GCT GGC GGC GTG C HSJGEBFRAS7 CCC BGB BCG BGB CCC GGB CCG BCB HSJGEBFRAS8 GGC CGT GGT TGG GGG TCT TC HSJGEBFRAS9 GCT GCC TCC GTT TGG GTG GC Human histidine decarboxylase: 5' -CTC TGT CCC TCT CTC TCT GTB CTC CTC BGG CTC CBT CBT CTC CCT TGG GC-3' des-adenosine antisense sequences : HUMHDCASl : TCT CCC TTG GGC TCT GGC TCC TTC TC (SEQ ID NO:13) HUMHDCAS2 : TCT CTC TCC CTC TCT CTC TGT HUMHDCAS3 : CGCCTCCGCCCTGGCTGCTGGGGTGGTGGTGC HUMHDCAS4 : TTT TGT TCT TCC TTG CTG CC HUMHDCAS5: GCC CCG CTG CTT GTC TTC CTC G Human beta tryptase: 5' -GGG CCT GGC CTG GGG CBG GGG CCG CGT BGG CGC GGC TCG CCB GGB CGG GCB GCG CCB GCB GCB GCB GBT TCB GCB TCC TGG-3' des-adenosine antisense sequences : HUMBTRYPAS1: CTTGCTCCTGGGGGCCTCCTG (SEQ ID NO:14) HUMBTRYPAS2 : GTC CCT CCG GGT GTT CCC GGC Human tryptase-I: 5' -CCT GGB CTG GGG CBG GGG CCG CGT BGG CGC GGC TCG CCB GGB CGG GCB GCG CCB GCB GCB GCB GGC TCB GCB TCC TGG CCB CGG BBT TCC-3' des-adenosine antisense sequences : HUMTRYAS1: CTTGCTCCTGGGGGCCTCCTG (SEQ ID NO:15) HUMTRYAS2: GTC CCT CTG GCT G TT CCC GGC Human prostaglandin D synthase: 5' -CCC CBG CBG GBC CBG TCC CBT CCB CBG CGT GTG BTG BGT BGC CBT TCT CCT GCB GCC GBG-3' des-adenosine antisense sequences : HUMPROSYNAS1:GGTGTGCGGGGCCTGGTGCC(SEQIDNO:16) HUMPROSYNAS 2: CCT GGG CCT CGG GTG CTG CCT GT HUMPROSYNAS 3: GCG CTG CCT TCT TCT CCT GG HUMPROSYNAS 4: 5' -GTC CTC GCC GGG GCC CTT GCT GCC CTG GCT GT -3' HUMPROSYNAS 5: GCC CTG GGG GTC TGG GTT CGGCTGT Human cyc1ooxygenase-2: 5' -TGB GCG CCB GGB CCG CGC BCB GCB GCB GGG CGC GGG CGB GCB TCG CBG CGG CGG GCB GGG-3' des-adenosine antisense sequences : HUMCYCLOXAS1: GGGCGCGGGCGBGCBTCGC(SEQ ID NO:17) HUMCYCLOXAS2 : TTT GGG CTT TTC TCC TTT GGT T Human eosinophil cationic protein: 5' -CBG BCB BBT TTG GGB BGT GBB CBG TTT TGG BBC CBT GTT TCC CBG TCT CTG BGC TGT GGC-3' des-adenosine antisense sequences : HSECPASl: CCTCCTTCC TGG TCT GTC TGC (SEQ ID NO:18) Human eosinophil derived neurotoxin: 5' -CCC CBB CBG BBG BBG CBG BCB BBT TTG GGB BGT GBB CBG TTT TGG BBC CBT GTT TCC TGT-3' des-adenosine antisense sequences : HSEOSDNASl : GCC CTG CTG CTC TTT CTG CT (SEQ ID NO:19) HSEOSDNAS 2 TCC CTT GGT GGG TTG GGC C HSEOSDNAS 3 GCT GGT TGT TCT GGG GTT C HSEOSDNAS 4 TTG CTG CCC CTT CTG TCC C HSEOSDNAS 5 TGT TTG CTG GTG TCT GCG C Human eosinophil major basic protein: GGG GGB GTT TCB TCT TGG CTT T des-adenosine antisense sequences , TCT CCC CTT GTT CCT CCC C TCT CCT GCT CTG GTG TCT CCT C TTC CCT CCC TCC CCT GCC GTG TTG TCT GTG GGT GTC C GTT TCG CTC TTG TTG CCC TGG GCC CTT CCC TGC TGG Human eosinophil peroxidase: 5' -GCB CCG TCC BGT GBT GGT GCG GTB CTT GTC GCT GCB GCG CTC GGC CTG GTC CCG GBG BGC-3' des-adenosine antisense sequences : HSEPAS1 GCGCTCGGCCTGGTCCCGG (SEQ ID NO:20) HSEPAS2 GGG TCT CCT CTT GTT GTT GC HSEPAS3 TTG CGC CTC CTG CTG GGG GT CC HSEPAS4 CTC TGT TCT TGT TTT GGG GGC HSEPAS5 GGG CCC GGC CGT TGT CTT G HSEPAS6 GTT TGG GGG TTT CCG TTG HSEPAS7 GGG TTC TCC TGG CCC GGG CCT TGC CC HSEPAS8 GGC CGT GGT CCC GGC TTC GTT GC HSEPAS9 CCT GTC TCC GTC TCG GCT CTT CTG HSEPAS10 : GGG CCT TGC GCT GTC TTT GGT G Human intercellular adhesion molecule-1 (CAM-1) : 5' - CGG BGC CTC CCC GGG GCB GGB TGB CTT TTG BGG GGG BCB CBG BTG TCT GGG CBT TGC CBG GTC CTG GGB BCB GBG CCC CGB GCB GGB CCB GGB GTG CGG GCB GCG CGG GCC GGG GGC TGC TGG GBG CCB TBG CGB GGC TGB G-3' des-adenosine antisense sequences : HSICAM1AS1: GCGCGGGCCGGGGGCTGCTGGG (SEQ ID NO:21) HSICAM1AS2 GGT TGG CCC GGG GTG CCC C HSICAM1AS3 GCC GCT GGG TGC CCT CGT CCTCTGCGGTC HSICAM1AS4 GTG TCT CCT GGC TCT GGT TCC CC HSICAM1AS5 5' -GCT GCG CCC GTT GTC CTC TGG GGT GGCCTTC-3' HSICAM1AS6 GCT CCC GGG TCT GGT TCT TGT GT HSICAM1AS7 TGG GGG TCC CTT TTT GGG CCT GTT GT HSICAM1AS8 GGC GTG GCT TGT GTG TTC GGT TTC HSICAM1AS9 TGC CCT GTC CTC CGG CGT CCC Human vascular cell adhesion molecule 1 (VCAM-1) : 5' -CTG BGC BBG BTB TCT BGB TTC TGG GGT GGT CTC GBT TTT BBBB GCT TGB GBB GCT GCB BBC BTT BTC CBB BGT BTB TTT GBG GCT CCB BGG BTC BCG BCC BTC TTC CCB GGC BTT TTB BGT TGC TGT CGT -3' des-adenosine antisense sequences : HSVCAMIASI CCTCTTTTCTGTTTTTCCC (SEQ ID NO:22) HSVCAM1AS2 CTC TGC CTT TGT TTG GGT TCG HSVCAM1AS3 CTT CCT TTC TGC TTC TTC C HSVCAM1AS4 CTGTGTCTCCTGTCTCCGCTTTTTTCTTC HSVCAM1AS5 GTC TTT GTT GTT TTC TCT TCC TTG Human endothelial leukocyte adhesion molecule (ELAM-1) : 5' -BBG TGB GBG CTG BGB GBB BCT GTG BBG CBB TCB TGB CTT CBB GBG TTC TTT TCB CCC -3' des-adenosine antisense sequences . HUMELAM1AAS1 GTTCTTGGCTTCTTCTGTC(SEQ ID NO:23) HUMELAM1AAS2 CGT TGG CTT CTC GTT GTC CC HUMELAM1AAS3 TGT GGG CTT CTC GTT GTC CC HUMELAM1AAS4 CCC TTC GGG GGC TGG TGG HUMELAM1AAS5 GGC CGT CCT TGC CTG CTG G Human P Selectin: des-adenosine antisense sequences : HUMPSELECTAS1: CTCTGCTGGT TTTCTGCCTT CTGCCC (SEQ ID NO:24) Human endothelial monocyte activating factor: des-adenosine antisense sequences : HUMEMAPIIAS1: 5' -TTT TCT CTT TCG CTT TCT TTT CGTCTCCTGTTCCTCCTTTT-3' (SEQ ID NO:25) HUMEMAPIIAS2: 5' -TTG CTG TTT TTT CTC CTT CTT CTC TCC TTT CTT TTC -3' Human IL3 -GGCGGBCCBGGBGTTGGBGCBGGBGCBGGBCGGGCB GGCGGCTCBTGTTTGGBTCGGCBGGBGGCBCTC -3' des-adenosine antisense sequences : HUMIL3AAS1 : 5' -CTC TGT CTT GTT CTG GTC CTT CGT GGG GCT CTG (SEQ ID NO:26)-3' HUMIL3AAS2: TGT CGC GTG G GTG CGG CCG TGG CC Human IL3 receptor: 5' -GCBGGBGBCBGGGCBGGGCGBTCBGGBGCBGCGT GBGCCBBBGGBGGBCCBTCGGGBBCGCBGCTCCG GBBCGCBGGBCBGBGGTGCC-3' des -adenosine antisense sequences: TCTGGGGTGTCCTG GCCTTCGTGGTTCC TCTTCCTTCGTTTGC CGTCCGCGGGGGCCCCCGGGCCT GGCTGCGCTCCTGCCCCGC CTCTTTCCCGGGCTCTT GCGCTGGGGGGTGCTCC CGTGTGTTTGCGCCCTCCTCCTGGTCGC GCTTGTCGTTTTGG GGCCGGCTTTGCCCGCCTCCC GGCGCCTGGCCCGGCC TTCCTGGGCTGCGTGCGC GTTCTGTTCTTCTTCCTGGC Human IL4: 5' -GCCGGCBCBTGCTBGCBGGBBGBBCBGBGGGGGB BGCBGTTGGGBGGTGBGBCCCBTTBBTBGGTGTCGB-3' des-adenosine antisense sequences : HUMIL4AS1: CTC TGG TTG GCT TCC TTC-3' (SEQ ID NO:27) Human IL4 receptor: 5' -GTTCCCBGBGCTTGCCBCCTGCBGCBGGBCCBGGCBGCTC BCBGGGBBCBGGBGCCCBGBGCBBBGCCBCCCCBTTGGGBG BTGCCBBGGCBCCBGGCTG-3' des-adenosine antisense sequences : TCTGCGCGCCCCTGCTCC CGCCCGGCTTCTCT CGTGTGGGCTTCGG CCCCGCGCCTCCGTTGTTCTC TGCTCGCTGGGCTTG GGTTTCCTGGGGCCCTGGGTTTC TCTGCCGGGTCGTTTTC GGGTGCTGGCTGCG CTTGGTGCTGGGGCTCC GGCGGCTGCGGGCTGGGTTGGG CTTGGCTGGTTCCTGGCCTCGGG CCTCCTCCTCCTCCTC GCTCCCTTTTTCTTCCTCT TCCCTGCTGCTCTC TGCCCTCCCTTCCCTCCTGG GGTGCCTCCTTGGGCCCTGC GGCTGCTCCTTGCCCC CTCTGGGTCGGGCTGGC GGGGCGTCTCTGTGC CTGGCCTGGGTGCC GCCTCTCCTGGGGG GGTGGCTCCCTGTCC CCTTTTCCCCCGGCTCC GTGGGGGCTTTGGC GGGGGTCTGTGGCCTGCTCCTGGGG AGGGGTCTGGGGCCCTC TTTTGGGGGTCTGGCTTG GCCTGGCTGCCTTCC GGGGCCTGCCGTGGGGC TGTCCTCTGTTGCTCCCCTT TGCCTGCTGTCTGG GGTTCCCGCCTTCCCT Human IL5: 5' -GTGGGBBTTTCTGTGGGGBTGGCBTBCBCGTBGGCB GCTCCBBGBGCTBGCBBBCTCBBBTGCBGBBGCBTC CTCBTGGCTCTGBBBCG -3' des-adenosine antisense sequences : HUMIL5AS1 TCC CTG TTT CCC CCC TTT (SEQ ID NO:28) HUMIL5AS2 CGT TCT GCG TTT GCC TTT GGC HUMIL5AS3 GTT TTT TGT TTG TTT TCT HUMIL5AS4 CTC TCC GTC TTT CTT CTC c HUMIL5AS5 CCT CCT GCC TGT GTC CCT GCT CCC C HUMIL5AS6 GAG GGT TTC TGG CTT CCT CTC T HUMIL5AS7 TGT CTC TCT GTC CTT TTG TT HUMIL5AS8 5' -TGT TGT GCG GCC TGG TGC TGC CCT GCCCCG GG-3' Human IL5 receptor antisense oligonucleotide 5' -CTCBGTGGCCCCCBBBBGGBT GBGTBBTBCBTGCGCCBCGBT GBTCBTBTCCTTTTTBCTBTGBGG-3' des-adenosine antisense sequences : CCGTGTCTGTCGTGTCT TTCCTTTGCTCTTG GTGTGTCTTTGCTGT GCCCTGCCTCTCTGC Human IL6 5' -CTCCTGGGGGTBCTGGGGCBGGGBB GGCBGCBGGCBBCBCCBGGBGCBGC CCCBGGGBGBBGGCBBCTGGBCCGB BGGCGCTTGTGGBGBBGGBGTTCBT BGCTGGGCTCCTGGBGGGGBGBTBGBGC-3 des-adenosine antisense sequence: HUMIL6AS1: GCT TCT CTT TCG TTC CCG GTG GGC TCG (SEQ ID NO:29) HUMIL6AS2 GTG GCT GTC TGT GTG GGG CGG CT HUMIL6AS3 GTG CCT CTT TGC TGC TTT C HUMIL6AS4 GAT TCT TTG CCT TTT TCT GC Human IL6 receptor antisense oligonucleotides 5' -GCBCGCCTCTTGCCBCCTCCTGCGCBGGGCB GCGCCTTGGGGCCBGCGCCGCTCCCGGCGCG GCCBGCBGGGCBGCCBGCBGCGCGCBGCCGB CGGCCBGCBTGCTTCCTCCTCGGCTBCCBCT CCBTGGTCCCGCBGBGGCGGBCBGGC-3' des-adenosine antisense sequences : GGGGGTGGCTTCCTGCC GCGTCTCTGGGCCGTCCC GTCCCTCGGCCCCGCGCCGCGCTCGGCTCCTCTCCC TCTGGCCCGGCTC GGGGCGGGGCGGGGCGGTGGGCGGGC GGCGCTGCCCTGCGC 5 GCGGCGCTGGCCCC TGCTGGCCGTCGGCTGCGCGCTGCTGGCTGCCCT GCTGGCCGCGCCGGG 10 GCCTGTCCGCCTCTGCGGG CGCTGTCTCCTGGC TTGTCTTCCGGCTCT TCTGCTGGGGTGGG 15 GCTGGGCGGCCGGCCCGGT GCTGGGGCTCCTCGGGGGG 20 GGGGGCTCTTCCGG GCTGTCTCCCTCCGGG GCGGGGGTTTCTGGCC 25 GTGGGGGTCTTGCC TGGCCTCCGGGCTCC 30 TGCTTGTCTTGCCTTCCTTC TCTGGTCGGTTGTGGCTCG GGGCTCCGTGGGTCCCTGGC 35 GCCCGTTTGTGTTTTGTC TTTTCCCCTGGCGT 40 CCCTGTGCCCCTCTCCTCTCCTTCCTCTGCTTCTC GCTCTCCTTTGTGGG GCCCTCCCTGCTGCT 45 CTTGGTTTTGGGCT TTTTTTCTCTTCCTCCTTTTTC 50 GTGCGTGGGCCTCC Human monocyte-derived neutrophil chemotactic factor: 5' -GGGGTGGBBBGGTTTGGBGTBTGTCTTTBTGCBCTGB CBTCTBBGTTCTTTBGCBCTCCTTGGCBBBBCTGCBC CTTCBCBCBGBGCTGCBGBBBTCBGGBBGGCTGCCBB GBGBGCCBCGGCCBGCTTGGBBGTCBTGTTTBCBCBC BGTGBGBTGGTTCCTTCCGG-3 ' des-adenosine antisense sequences : HSMDNCFASl: GCT TGT GTG CTC TGC TGT CTC T (SEQ ID NO:30) HSMDNCFAS2 : 5' -TGG TTC CTT CCG GTG GTT TCT TCC TGG CTC TTG TCC T -3' HSMDNCFAS3 : TTC TCT TGG CCC TTG GC Human neutrophil elastase (medullasin) : 5' -GGGCTCCCGCCGCGBGBGGTTBTGGGCTCCCBGGBCCBC CCGCBCCGCGCGGBCGTTTBCBTTCGCCBCGCBGTGCGC GGCCGBCBTGBCGBBGTTGGGCGCBBTCBGGGTGGCGCC GCBGBBGTGGCCTCCGCGCBGCTGCBGGGBCBCCBTGBB GGGCCBCGCGTGGGGCCGCGCTCGCCGGCCCCCCBCBBT CTCCGBGGCCBGCGCGGTGCCCCCCBGCBGCBBGGCCGG CBGGBCBCBGGCGBGGBGBCBCGCGBGTCGGCGGCCGBG GGTCBTGGTGGGGCTGGGGCTCCGGGGTCTCTGCCCCTC CGTGC-3' des-adenosine antisense oligonucleotides : HSMEDURAS1: 5' -TGG TGG GGC TGG GGC TCC GGG GTC TCT GCC CCT CCG TGC-3' (SEQ ID NO: 31) HSMEDURAS2 CGC GTG GGG CCG CGC TCG CCG GCCCCCC HSMEDURAS3 CCT GCC GGG TGG GCT CCC GCC GCG HSMEDURAS4 CGC CGG CCT GCC GGC CCC TC HSMEDURAS5 5' -GTG GGT CCT GCT GGC CGG GTC CGG GTC CCG GGG GTG GGG-3' HSMEDURAS6: CGC GBG TCG GCG GCC GBG GGT C Human neutrophil oxidase factor: 5 ' -CGGGBGTGGGGGTCCTGGBCGGCBCTGBBGGCBTCCBGGG CTCCCTTCCBGTCCTTCTTGTCCGCTGCCBGCBCCCCTTC BTTCCBGBGGCTGBTGGCCTCCBCCBGGGBCBTGBTTBGG TBGBBBCTBGGBGGCC-3 ' des-adenosine antisense sequence : HUMNOXFAS1: GGC CTC CBC CBG GGB CBT G (SEQ ID NO:32) HUMNOXFAS2 : GTC CTT CTT GTC CGC TGC C HUMNOXFAS3 : TCT CTG GGG TTT TCG GTC TGG GTG G HUMNOXFAS4 : GCT TTC CTC CTG GGG CTG CTG CTG HUMNOXFAS5 : 5' -GGC TCT TCT TTT TGT TTC TGG CCT GGTG-3' HUMNOXFAS6 : CTC TCT CGT GCC CTT TCC HUMNOXFAS7 : CTT GGG TGT CTT GTT TTT GT HUMNOXFAS8 : 5' -GGCCTCCBCCBGGGBCBTGGTCCTTCTT GTCCGCTGCC -3' Human cathepsin G: 5' -CCCTCCBCBTCTGCTCTGBCCTGCTGGBCTCTG GBTCTGBBGBTBCGCCBTGTBGGGGCGGGBGTG GGGCCTGCTCTCCCGGCCTCCGBTGBTCTCCCCT GCCTCBGCCCCBGTGGGTBGGBGBBBGGCCBGCB GBBGCBGGBGTGGCTGCBTCTTTCCTG -3' des-adenosine antisense sequences : HUMCTHGASl: GTG GGG CCT GCT CTC CCG GCC TCC G (SEQ ID NO:33) HUMCTHGAS2: TGTGTTGCTGGGTGTTTTCCCGTCTCTGG HUMCTHGAS3 : TCT GCC TTC GGG GGT CGT Human defensin 1: 5' -CCGGGGCTGCBGCBBCCTCBTCBGCTCTTGCCT GGBGTGGCTCBGCCTGGGCCTGCBGGGCCBCCB GGBGBBTGGCBGCBBGGBTGGCGBGGGTCCTCB TGGCTGGGGTCBCBGBTCCTCTBGCTBGGCBGG GTGBCCBGBGBGGGC-3' des-adenosine antisense sequences : HUMDEFIAAASI : GGG TCC TCB TGG CTG GGG (SEQ ID NO:34) HUMDEF1AAAS2 GCC TGG GCC TGC BGG GCC HUMDEF1AAAS3 GCT CTT GCC TGG BGT GGC TC HUMDEF1AAAS4 GCC CBG BGT CTT CCC TGG T Human defensin 3 : 5' -CGCTGCBBTCTGCTCCGGGGCTGCBGCBBCCTCBTC BGCTCTTGCCTGGBGTGGCTCBGCCTGGGCCTGCBG GGCCBCCBGGBGBBTGGCBGCBBGGBTGGCGBGGGT CCTCBTGGCTGGGGTCBCCTGGBGGBGGGBGBGCBGG-3' des-adenosine antisense sequences : HUMNTRIIIASl: GGG TCC TCB TGG CTG GGG TC (SEQ ID NO:35) HUMNTRIIIAS2: CCT CTC TCC CGT CCT Human macrophage inflammatory protein-1-alpha: RANTES RECEPTOR 5' -GBGGGGGCBGCBGTTGGGCCCCBBBGGCCCTCTCGT TCBCCTTCTGGCBCGGBGTTGCBTCCCCBTBGTCBB BCTCTGTGGTCGTGTCBTBGTCCTCTGTGGTGTTTG GBGTTTCCBTCCCGGCTTCTCTCTGGTTCCBBGGGB-3' des-adenosine antisense sequences : HUMRANTESASl: GTC TTT GTT TCT GGG CTC GTG CC (SEQ ID NO:36) HUMRANTESAS2 CCB TCC CGG CTT CTC TCT GGT TCC HUMRANTESAS3 GTC CTCTGT GGT GTT TGG HUMRANTESAS4 5' -CCC TGC TTC CTT TTG CCT GTT TCTTTGTTT CTGGGCTCGT GCC RANTES: 5' -GGGCBCGGGGCBGTGGGCGGGCBBTGTBGGC BBBGCBGCBGGGTGTGGTGTCCGBGGBBTBTGGG GBGGCBGBTGCBGGBGCGCBGBGGGCBGTBGCBB TGBGGBTGBCBGCGBGGCGTGCCGCGGBGBCCTTC BTGGTBCCTGTGGBGBGGCTGTCGGBGG-3' des-adenosine antisense sequences : GGGTGTGGTGTCCG CTTGGCGGTTCTTTCGGGTG TTTCTTCTCTGGGTTGGC CTGCTGCTCGTCGTGGTC GCTCCGCTCCCGGGTTC GTCTCGCTCTGTCGCCC CTTCCTTCCTTGTC GTGTTCCTCCCTTCCTTGCCTCT Human muscarinic acetylcholine receptor HM1: des -adenosine antisense sequences : HSHMIASI: GTT CBT GGT GGC TBG GTG GGG C (SEQ ID NO:37) HSHM1AS2: GCT GCC CGG CGG GGT GTG CGC TTG GC HSHM1AS3: GCTCCCGTG CTC GGT TCT CTG TCTCCCGGT HSHM1AS4 : CCC CCT TTG CCT GGC GTC TCG G HSHM1AS5: GCC TTC GTC CTC TTC CTC TTC TTC CTTCC HSHM1AS6: 5' -GCT CCG TGG GGG CTG CTTGGTGGG GGCCTG TGC CTC GGG GTC C-3' HSHM1AS7: CGG GGC TTC TGG CCC TTG CC Human muscarinic acetylcholine receptor HM3 : des-adenosine antisense sequences : HSHM3AS1: GGG GTG GGT BGG CCG TGT CTG GGG (SEQ ID NO:38) HSHM3AS2 GTT GGC CBT GTT GGT TGC C HSHM3AS3 TCT TGG TGG TGC GCC GGG C HSHM3AS4 5' -GCG TCT TGG CTT TCT TCT CCT TCG GGC CCT CGG GCC GGT GCT TGT GG-3' HSHM3AS5: 5' -GCT CCT CCC GGG CGG CCT CCC CGG GCG GGG GCT TCT TG-3' HSHM3AS6: GCG CTG GCG GGG GGG CCT CCT CC HSHM3AS7: 5' -GCT CTG TGG CTG GGC GTT CCT TGG TGT TCT GGG TGG C-3' HSHM3AS8: TGG CGG GCG TGG TGG CCT CTG TGG TGG HSHM3AS9: GGG CCC GCG GCT GCB GGG G HSHM3AS10: TTG CCT GTC TGC TTC GTC HSHM3AS11 : CTT TGC GCT CCC GGG CCG CC Human fibronectin: des-adenosine ant lsense sequences : HUMFNA/HSFIB1AS1 CGG TTT CCT TTG CGG TC (SEQ ID NO:39) HUMFNA/HSFIB1AS2 TTG GCC CGG GCT CCG GGT G HUMFNA/HSFIB1AS3 CCC GCC CGC CCG CCG GCC GCCGC HUMFNA/HSFIB1AS4 5' -CCC GCC GGG CTG TCC CCG CCC CGC CCC-3' HUMFNA/HSFIB1AS5 GGC CCG GGG CGC GGG GG HUMFNA/HSFIB1AS6 CGG CCC TCC CGC CCC TCT GG HUMFNA/HSFIB1AS7 GCC GGC GCG GGC GTC GG HUMFNA/HSFIB1AS9 5' -CCG CTC GCG CCT GGG GTT CCC TCT CCT CCCCCTGTGC-3' HUMFNA/HSFIB1AS10 GCC TGC CTC TTG CTC TTC HUMFNA/HSFIB1AS11 TGC GTC CGC TGC CTT CTC CC HUMFNA/HSFIB1AS12 CTC TCC TCG GCC GTT GCCTGTGC HUMFNA/HSFIB1AS13 5' -TGT CCG TCC TGT CGC CCT TCC GTG GTG C-3' HUMFNA/HSFIB1AS14 TGT TGT CTC TTC TGC CCT C HUMFNA/HSFIB1AS15 GGT GTG CTG GTG CTGGTGGTGGTG HUMFNA/HSFIB1AS16 CCT CTG CCC GTG CTC GCC HUMFNA/HSFIB1AS17 CTG CCT GGG CTG GCCTCTTCGGGT HUMFNA/HSFIB1AS18 5' -GTG GCT TTG GGG CTC TCT TGG TTG CCC TTT-3' HUMFNA/HSFIB1AS19 5' -CTT CTC GTG GTG CCT CTC CTC CCT GGC TTG GTC GT-3' HUMFNA/HSFIB1AS20 TGT CTG GGG TGG TGCTCCTCTCCC HUMFNA/HSFIB1AS21 TTT CCC TGC TGG CCG TTT GT HUMFNA/HSFIB1AS22 CCT GTT TTC TGT CTT CCT CT HUMFNA/HSFIB1AS23 TTC CTC CTG TTT CTC CGT HUMFNA/HSFIB1AS24 5' -TTG GCT TGC TGC TTG CGG GGC TGT CTC C-3' HUMFNA/HSFIB1AS25 CTT GCC CCT GTG GGC TTT CCC HUMFNA/HSFIB1AS26 TGG TCC GGT CTTCTCCTTGGGGGTC HUMFNA/HSFIB1AS27 GCC CTT CTT GGT GGG CTG HUMFNA/HSFIB1AS28 GCT CGT CTG TCT TTT TCC TTCC HUMFNA/HSFIB1AS29 5' -TGG GGG TGG CCG TTG TGG GCG GTG TGG TCC GCC T-3' HUMFNA/HSFIB1AS30 TGC CTC TGC TGG TCT TTC Human interleukin 8: 5' -GBTGTTTGTTBCCBBBGCBTCBBGBBTBGCTTTGC TBTCTBBGGBTCBCBTTTBGBCBTBGGBBBBCGC TGTBGGTCBGBBBGBTGTGCTTBCCTTCBCBCBG BGCTGCBGBBBTCBGGBBGGCTGCCBBGBGBGCC BCGGCCBGCTTGGBGTCBTGTTTBCBCBCBGTGBG-3' des-adenosine antisense sequences : HUMIL8AAS1: GTG CTC CGG TGG CTT TTT (SEQ ID NO:40) HUMIL8AAS2 : GCT TGT GTG CTC TGC TGT CTC TG HUMIL8AAS3 : 5' -TTC CTT CCG GTG GTT TCT TCC TGG CTC TTG TCC T-3' HUMIL8AAS4 : TTC TCT TGG CCC TTG GCC C Human IL-8 receptor-alpha 5' -BCBGGGGCTGTBBTCTTCBTCTGCBGGTGGCB TGCCBGTGBBBTTTBGBTCBTCBBBBTCCCBCBT CTGTGGBTCTGTBBTBTTTGBCBTGTCCTCTTC BGTTTCBGCBBTGGTTTGBTCTBBCTGBBGCBCCG GCCBGG-3' des-adenosine antisense sequences : TGGCTCGGTGCTTCTGCCCC TGTTGTTGCGGCGCTC GGTTGGTGTGGCCCCTG TGGTGCTTCGTTTCC CCCTCTTTCTCTTTGTTC GGGGGTTCTTGTGGC GGGCTGCTTGTCTCGTTCC Human GM-CSF: 5' -CTTGBGCBGGBBGCTCTGGGGCBGGGBGCTGGCBG GGCCCBGGGGGGTGGCTTCCTGCBCTGTCCBGBGT GCBCTGTGCCBCBGCBGCBGCTGCBGGGCCBTCBG CTTCBTGGGGCTCTGGGTGGCBGGTCCBGCCBTGG GTCTGGGTGGGGCTGGGCTGCBGGCTCCGGGC-3 ' des-adenosine antisense sequences : HUMGCSFAS1:GGT CCB GCC BTG GGT CTG GG (SEQ ID NO:41) HUMGCSFAS2 GGC TGG GCT GCB GGC TCC GG HUMGCSFAS3 GCG GGC GGG TGC GGG CTG CGT GCT GGG HUMGCSFAS4 GGC TGC CCC GCA GGC CCT GC Human tumor necrosis factor α : 5' -CBCCGCCTGGBGCCCTGGGGCCCCCCTGTCTTCTTGGG GBGCGCCTCCTCGGCCBGCTCCBCGTCCCGGBTCBTGCTTT CBGTGCTCBTGGTGTCCTTTCCBGGGGBGBGBGGG-3 ' des-adenosine antisense sequences HSTNFAAS1 : GCT GGT CCT CTG CTG TCC TTG CTG (SEQ ID NO 42) HSTNFAAS2 : GTG CTC BTG GTG TCC TTT CC HSTNFAAS3 : GCC CTG GGG CCC CCC TGT CTT CTT GGGG HSTNFAAS4 : CCT CTT CCC TCT GGG GGC CG HSTNFAAS5 : TCT CTC TCC CTC TCT TGC GTC TCT C HSTNFAAS6 : TCT TTC TCT CTC TCT CTT CCC c HSTNFAAS7 : TTT CCC GCT CTT TCT GTC TC HSTNFAAS8 : GGT GTC TGG TTT TCT CTC TCC HSTNFAAS9: GCT GGC TGC CTG TCT GGC CTG CGC TCTT HSTNFAAS10 GGC CTG TGC TGT TCC TCC HSTNFAAS11 TCC GGT TCC TGT CCT CTC TGT CTG TC HSTNFAAS12 GCC CCC TCT GGG GTC TCC CTC TGG C HSTNFAAS13 GTG GTG GTC TTG TTG CTT HSTNFAAS14 GGG CTG GGC TCC GTG TCT C HSTNFAAS15 CBG TGC TCB TGG TGT CC HSTNFAAS16 GCT GBG GGB GCG TCT GCT GGC Human leukotriene C4 synthase: 5' -CTCGGTBGBCGCGCTCGBBCTCGGGTGGGCCGGTGGTG BGCGGCGGCGBCBCGCGGBBGGCCCTGCGCGCCGBGBTCBC CTGCBGGGBGBBGTBGGCTTGCBGCBGGBCTCCCBGGBGGG TGBCBGCBGCCBGTBGBGCTBCCTCGTCCTTCBTGGTBCCG TCGGTGTGGTGGCBCGGGCTGTGTGTGBBGGCGBGCTGG-3' des-adenosine antisense sequences : HSU11552AS1:GCC CCG TCT GCT GCT CCT CGT GCC G (SEQ ID NO:43) HSU11552AS2: 5' -CCT CGT CCT TCA TGG TAC CGT CGGTGT GGT GGC-3' HSU11552AS3 CTC GGG TGG GCC GGT GGT G HSU11552AS4 GGG CGC GCG CGC TCG CGT HSU11552AS5 5' -GGC TCC GGC TCT TCT TTC CCG GCTCCG TCG GCC CGG GGG CCTTGGTCTC-3' HSU11551AS6:CCT CGT CCT TCB TGG TBC CG Human Endothelin-1: 5' -BCCGGCGGBGCCGCCBGGGTGGBCTGGGBGTGGGTT TCTCCCCGCCGTTCTCBCCCBCCGCGCTGBGCTCBGCGC CTBBGBCTGCTGTTTCTGGBGCTCCTTGGCBBGCCBCBB BCBGCBGBGBGBBBBTCBTGBGCBBBTBBTCCBTTCTGB BBBBBBGGGBTCBBBBBCCTCCCGT-3' des -adenosine antisense sequences : CCCGTTCGCCTGGCGC GCGCTGCGGGTTCCTC GTGGGTTTCTCCCCGCCGTTCTC CGGTCTGTTGCCTTTGTGGG CTTCTTGTCTTTTTGGCT GTTCTTTTCCTGCTTGGC GTCTTTTCCTTTCTT TGTGCTCGGTTGTGGGTC CGCTGGTCCTTTGCC CTGTGTGTTTCTGCTG Endothelin receptor ET-B antisense oligonucleotides 5' -GCCCTGTCGGGCGGGBBGCCTCTCTCCTCTCCCCBG BTCCGCGBCBGGCCGCBGGCBBGBBCCBGCGCBBCCBGG GCGCGTCCGCBCBGBCTTGGBGGCGGCTGCBTGCTGCTB CCTGCTCCBGBBGCGTCCGGTGGCCGCCGC-3' ■ des-adenosine antisense sequences : GCGTCCGGTGGCCGCCGC GCCTCTCTCCTCTCCCC GTGGCCCTGTCGGGCGGG TCCTGCCGTCCTGTCTCCTTT TCTTTTGCTGTCTTGT CTTCCCGTCTCTGCTTT Endothelin ETA receptor antisense oligonucleotides 5' -CBTCCBCBTGBTTGCTTBGBTTTGTGCTGTBTCTCTCB GGBTTBTCBCTGBTTBCBCBTCCBBCCBGTGCCBGCCBBBB GGBTGCCCTGBGGCBBBGGGTTTCCBTCTTGBGGCBBBTTT GBGGB-3' des-adenosine antisense sequences : GTCTGTCCTCCCCGTCTCCTCCC ACTGCTTCTCCCGGGG GCTTCCCCGGCTTC GGGTGGCCGGTGTCCCGGGCTCCGGCGCGGCGGC GGCTTCGGCTGC GGGTGGGTGGCGCGG GCTGCCGGGTCCGCGCGGCGCCTGGGCC CTTGTGCTGCTTTT TGCTTGTTCCGTTC TGGCTGCTCCGGTCTGTGTTGTGGTTGTTTTG TTTCTTCTTGGGTGTGGG CCTTGCGGTTTTGG CTGTGGGCCCTTTG GGGCCTTGGCTTCTGGCTC Substance P antisense oligonucleotide 5' -CTGCTGBGGCTTGGGTCTCCGGGCGBTTCTCTGCBGBBGBT GCTCBBBGGGCTCCGGCBGTTCCTCCTTGBTCTGGTCGCTGTCG TBCCBGTCGGBCCBGTBBTTCBGBTCBTCBTTGGCTCCTBTTTC TTCTGCBBBCBGCTGBGTGGBGBCBBGBBBBBBGBCTGCCBBGG CCBCGBGGBTTTTCBTGTTGGBTTTTGCGBCGGBCBGTCCCGCG GGGTGCTGAGTTTCTCTGGTTCCTCCGBGCGCB-3' des-adenosine antisense sequences : CGTGGTCGCTCCGC TTTCTCTGGTTCCTCCG GTCCCGCGGGGTGCTG TCTGGTCGCTGTCGT GGCTTGGGTCTCCGGGCG GTTTCCTTCCTTTTCCGC Substance P receptor antisense oligonucleotide 5' -GGCTBBGBTGBTCCBCBTCBCTBCCBCGTTGCCCBCCBCB GBGGTCBCCBCBBTGBCCGTGTBGGCBGCTGCCCBBBGGBCBB TTTGCCBGGCTGGTTGCBCGBBCTGBTTGGGTTCCGBGGTGTT BGTGGBGBTGTTTGGGGBGBGGTCTGBGTCCBCCGGGBGGBCG TTBTCCBTTTCGBBGCTBGGCGGTBBBGCCCTBCTBTCTGTBC BCBBCCCCCCTCTGCBGCBGBGTCCTGTCGTGGCGCCTGGGGC TCBGGGTCC-3' des-adenosine antisense sequences : GTCCTGTCGTGGCGCCTGGGGCTC TTCTTTTGTGGGCT CTTTGGTGGCTGTGGCTG TGGTCTCTGTGGTTG CTGCCCTGGGTCTGG GGGTGTGGCCTTGGGGCCGTCCTCTGGCTCCTCCTCGTGGGCCCCC Chymase 5' -GGBGCTGBTBCTGCBGATTTCBGBGGGBBGBBCCCT GBTBCTCBCCBGCTTCBGCTCTGGBGCBCBBGBGBBBGB GCBGCBGGGGGBGBGGBBGBBGCBGCBTCTTCCCBGBGB GGCTGCCTGBGCBBBTGCTGGTTTTCCTTTCCBGTCTTG GGTTTTBTBBCTCCCBGBBGGCBBGBGBGGGGCBBGG-3 ' des-adenosine antisense sequences : CGTTTTCTTCTCTC TGCTGGTTTTCCTTTCC TGGCAGTGGGTGGGGGTGGGGGTGGGGTGGC TTCCTTGTTCCTGGGGGTGTCCT CTTGCTCTGGGCTTTTCT CCCCTTTTCCTTCC TGTCTGTTTTCCTGGGG ' CTCTCCTCTGTCTCTGTGT CCTTGCCCTGGCCC TCTTCCCTCTCCTGTCTCCTGT CCCTGTGTTCCGCCC GTCTTCCCTCTCCTG ACCTCCTTTTCCTCCG CTGGGTGGGGCCCTG CCTGTTCTCTGCTCCC TGGCTTGGGGTTTCTTCTG TGTGTCTTCTTCCTCTGTT GGCTGGCTTTCTCCTTC TTTTGTCTTCCTGGG TGCCCCTTCTTCCTTTCTTGGG TCCTTGGTGCTTGGGCTGGG Endothelial nitric oxide synthase 5' -GCGTCTTGGGGTGCBGGGCCCBTCCTGCTGCGCCTGGGCG CTGBGGGTGTCBTBGGTGBTGCTCCCCBCCTCCCBGTTCTTCB CBCGBGGGBBCTTGGGCCCCTCTGGGGGCTGGGTTBGCGGGGB GCTCGGGGGGCTGTGTTCTGGCGCTGGTGGGBGTBGGGBTGCT GGGGCCCGGCTGGGCTCBGGGGCCGGGGTGGCTGGGCCCTGCT TGCCGCBCBGCCCBBGGCCCBGCCCCBGCCCCBGGCCGCBGGG TGGCCCBGGCTCCTGGGCCBCGCTCTTCBBGTTGCCCBTGTTB CTGTGCGTCCGTCTGCTGGBGCBGGCBGCBGBGTGGGBBTTC-3' des-adenosine antisense sequences : CTGTGCGTCCGTCTGCTGG GGGGCCGGGGTGGCTGGGCCCTGCTTGCCGC ACGACCCCGGGCCGACCCGAG GCTCGGGGGGCTGTGTTCTGGCGCTGGTGGG CTTGGGCCCCTCTGGGGGCTGGGTT TCCTGCTGCGCCTGGGCGCTG GCGTCTTGGGGTGC GGGGCCGGGGGGCCGGGGG GCCGCTGTTCGTGGGCCTGGG • GGTGCCTGTGGCTGCC GGTTGCCCCGGTTGGTGGC GCCGTCCTGCTGCCGGT CGTTGGCTGGGTCCCCCCGC 5 CCGTTTCCTGGGGTCC GCGTGGGGTGCTCC GGTTCCTCGTGCCG CTGCTGCCTTGTCTTTCC GGCCGTGGCGGCGTGGTGGTCC 10 GCCCCCCCTGGCCTTCTGCTC GGGGTCTGGCTGGT TGCCGGTGCCCTTGGCGGC GGTCTTCTTCCTGGTG GCTCTGGGCCCGGCCGGTCTCGG 15 GCGTCTCGTGTTCG CTCTTGTGCTGTTCCGGCCG CTCCTTCCTCTTCCGCCGCC GCCGCTCCCCGCCC 20 GCTCGTCGCCCTGGCCC GGCCTCCTCCTGGCCGC TGTCTCGGGCGGCGGCCTTGGC GCTCCGTTTGGGGCTG CCTCTGGCGCTTCC 25 GGCCCTCGGCCTGGGCGCTC TCTTCCGCCTGTGC TGGTGGCCCTCGTGG GCCCCTCCTGGCCTCCGGTGTCC TGTGGTCCCCCGGCTGGT • GGCCGGGCCGGTTGGGCGGGC GTGGGCGCCGGCGGGTCCTCC GGGCTGCCCTTCTCC GCCGGGGGTCCCGC GCTCCTGCTGTTCCCTGGGCTCTTCTGCC TCTCTCCTGGGTGGGTGCTGGGTGCCG GGGTCTCCGGGCTTG CCCCGCGCTGCTGGGCGTTCTGC GGTCTTGGGGTTGTC TGTGGCCCCGCTCG TGTCGCCCTCCGTCGCC CGTCGCCGGCCTCGTCC CCTCCTGGGTGCGC GGCGGGCTGGTCCT GGCGTTTTGCTCCTTCCTGG Inducible nitric oxide synthase 5' -CTGCCCCBGTTTTTGBTCCTCBCBTGCCGTGGGGBGGB CBBTGGGGTTGCBTCCBGCTTGBCCBGBGBTTCTGGBG BCTTCTTTCCCGTCTCCBCGBGGGGCTGCGGGGBCTCB TTCTGCTGCTTGCTGBGGTTGTGBTBCTGBGGTCBTCC TGTGTCBCTGGBCTGGBGGTGGCBCBGGGGGCTTTCTC CBCBTTGTTGTTGBTGTCTTTTTCCCCBTTCBTTGCBT BCTGGTGGBBTTTGGTCTTGBBCBGBBBTTTCCBBGGB CBGGCCBTCTCTBTGGCTTTBCBBBGCBGGTCBCTTBT GTCBCTTBTCTGGBTTTGBGCTCBGBTGTTCTTCBCTG TGGGGCTTGCBGCTGGCTGCBCTGCCTCCCCGGGGTB-3' Human major basic protein: GTTTCATCTT GGCTTTATCC (SEQ ID NO:44) EXAMPLE 6 Turning now to Figure 3, two asthmatic rabbits were adminstered adenosine, and two rabbits were adminstered dAMP, at the indicated concentrations, by inhalation as described above in Example 3. The results (shown in Figure 3 as change in compliance) indicate that dAMP, a breakdown product of antisense oligodeoxynucleotides containing adenosine, is as potent in the induction of bronchoconstriction as adenosine in the hyperresponsive airways of asthmatic rabbits. EXAMPLE 7 An aerosolized phosphorothioate 21-mer antisense ODN consisting of 50% adenosine and 50% guanine plus cytosine in a random configuation was found to produce potent bronchoconstrictor effects in hyperreactive airways of asthmatic rabbits, as illustrated in Figure 4. The control molecule used in this study, a phosphorothioate 21-mer antisense ODN consisting of 50% guanine and 50% thymidine plus cytosine (des-adenosine ODN) produced no bronchoconstrictor or any other effect in these same animals. In this study, bronchoconstrictor effects were measured as a percentage change in bronchial compliance. Each group consisted of two allergic rabbits, and data shown are for the period following the second of two daily administrations of 5 mg aerosolized ODN by nebulizer. These results indicate that antisense oligonucleotides, even when modified to slow degradation, produce adenosine metabolites capable of potent bronchoconstriction when adminstered in asthmatic airways. The foregoing examples are illustrative of the present invention, and are not to be construed as limiting thereof. The invention is defined by the following claims, with equivalents of the claims to be included therein. SEQUENCE LISTING (1) GENERAL INFORMATION: (l) APPLICANT: Nyce. Jonathan W (n) TITLE OF INVENTION: Method of Treatment of Lung Diseases Using Antisense Oligonucleotides (m) NUMBER OF SEQUENCES: 44 (iv) CORRESPONDENCE ADDRESS. (A) ADDRESSEE: Kenneth D. Sibley (B) STREET Post Office Drawer 34009 (C) CITY Charlotte (D) STATE. NC (E) COUNTRY. USA (F) ZIP: 28234 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0. Version #1.30 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (vin) ATTORNEY/AGENT INFORMATION: (A) NAME: Sibley, Kenneth D. (B) REGISTRATION NUMBER: 31.665 (C) REFERENCE/DOCKET NUMBER: 5218-32 (lx) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (919) 881-3140 (B) TELEFAX: (919) 881-3175 (C) TELEX: 575102 (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (n ) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION. SEQ ID NO:l. GATGGAGGGC GGCATGGCGG G 21 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:2: GTAGCAGGCG GGGATGGGGG C 21 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:3: GTTGTTGGGC ATCTTGCC 18 (2) INFORMATION FOR SEQ ID N0:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: li ear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:4: GTACTTGCGG ATCTAGGC 18 (2) INFORMATION FOR SEQ ID N0:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:5: GTGGGCCTAG CTCTCGCC 18 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:6: GTCGGGGTAC CTGTCGGC 18 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: TGCTTTTCTT πCTGGGCCT C 21 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 29 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:8: GGCGCCGTGC CGCGTCTTGG TGGCGGCGG 29 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9: TTTCCCCTGG GTCTTCC 17 (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: GCCTGTGTCT CTCCTCCT 18 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: GCCTTTCCTG GTTCTCTT 18 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION- SEQ ID NO 12 GCCTGTGTCT GTCCTCCT 18 (2) INFORMATION FOR SEQ ID NO:13: (i) S S: irs le (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: TCTCCCTTGG GCTCTGGCTC CTTCTC 26 (2) INFORMATION FOR SEQ ID NO:14: (l) SEQUENCE CHARACTERISTICS. (A) LENGTH: 21 base pairs (B) TYPE: nucleic aci d (C) STRANDEDNESS: single (D) TOPOLOGY: linear (π) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: CTTGCTCCTG GGGGCCTCCT G 21 (2) INFORMATION FOR SEQ ID NO:15: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: CTTGCTCCTG GGGGCCTCCT G 21 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: GGTGTGCGGG GCCTGGTGCC 20 (2) INFORMATION FOR SEQ ID NO:17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: isc feature (B) LOCATION: 12 (D) OTHER INFORMATION: /standard_name= "Reduced A" (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 15 (D) OTHER INFORMATION: /standard_name= "Reduced A" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: GGGCGCGGGC GAGCATCGC 19 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: CCTCCπCCT GGTCTGTCTG C 21 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19: GCCCTGCTGC TCTTTCTGCT 20 (2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: GCGCTCGGCC TGGTCCCGG 19 (2) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: GCGCGGGCCG GGGGCTGCTG GG 22 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22: CCTCTTTTCT GTTTTTCCC 19 (2) INFORMATION FOR SEQ ID N0:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:23: GTTCTTGGCT TCπCTGTC 19 (2) INFORMATION FOR SEQ ID NO:24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:24: CTCTGCTGGT TTTCTGCCTT CTGCCC 26 (2) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 41 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:25: TTΓTCTCTΓT CGCTTTCTTT TCGTCTCCTG TTCCTCCTTT T 4i (2) INFORMATION FOR SEQ ID N0:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 33 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: CTCTGTCTTG πCTGGTCCT TCGTGGGGCT CTG 33 (2) INFORMATION FOR SEQ ID NO:27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:27: CTCTGGTTGG CTTCCTTC 18 (2) INFORMATION FOR SEQ ID NO:28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:28: TCCCTGTTTC CCCCCTTT 18 (2) INFORMATION FOR SEQ ID NO:29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29: GCTTCTCTTT CGTTCCCGGT GGGCTCG 27 (2) INFORMATION FOR SEQ ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:30: GCTTGTGTGC TCTGCTGTCT CT 22 (2) INFORMATION FOR SEQ ID N0:31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31: TGGTGGGGCT GGGGCTCCGG GGTCTCTGCC CCTCCGTGC 39 (2) INFORMATION FOR SEQ ID N0:32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32: GTCCTTCTTG TCCGCTGCC 19 (2) INFORMATION FOR SEQ ID NO:33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:33: GTGGGGCCTG CTCTCCCGGC CTCCG 25 (2) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:34: GGGTCCTCAT GGCTGGGG 18 (2) INFORMATION FOR SEQ ID N0:35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 9 (D) OTHER INFORMATION: /standard name= "Reduced A" (xi) SEQUENCE DESCRIPTION: SEQ ID N0:35: GGGTCCTCAT GGCTGGGGTC 20 (2) INFORMATION FOR SEQ ID NO:36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:36: GTCTTTGTTT CTGGGCTCGT GCC 23 (2) INFORMATION FOR SEQ ID N0:37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 5 (D) OTHER INFORMATION: /standard_name= "Reduced A" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 14 (D) OTHER INFORMATION: /standard_name= "Redcued A" (xi) SEQUENCE DESCRIPTION: SEQ ID N0:37: GTTCATGGTG GCTAGGTGGG GC 22 (2) INFORMATION FOR SEQ ID N0:38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 10 " (D) OTHER INFORMATION: /standard name= "Reduced A" (xi) SEQUENCE DESCRIPTION: SEQ ID N0:38: GGGGTGGGTA GGCCGTGTCT GGGG 24 (2) INFORMATION FOR SEQ ID NO:39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pai s (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:39: CGGTTTCCTT TGCGGTC 17 (2) INFORMATION FOR SEQ ID N0:40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:40: GTGCTCCGGT GGCTTTTT 18 (2) INFORMATION FOR SEQ ID N0:41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 6 " (D) OTHER INFORMATION: /standard_name= "Reduced A" (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 10 " (D) OTHER INFORMATION: /standard_name= "Reduced A" (xi) SEQUENCE DESCRIPTION: SEQ ID N0:41: GGTCCAGCCA TGGGTCTGGG 20 (2) INFORMATION FOR SEQ ID N0:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:42: GCTGGTCCTC TGCTGTCCTT GCTG 24 (2) INFORMATION FOR SEQ ID N0:43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID N0:43: GCCCCGTCTG CTGCTCCTCG TGCCG 25 (2) INFORMATION FOR SEQ ID N0:44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 6 " (D) OTHER INFORMATION: /standard_name= "Reduced A" (ix) FEATURE: (A) NAME/KEY: misc feature (B) LOCATION: 17 (D) OTHER INFORMATION: /standard name= "Reduced A" (xi) SEQUENCE DESCRIPTION: SEQ ID N0:44: GTTTCATCTT GGCTTTATCC 20

Claims: THAT WHICH IS CLAIMED IS: 1. A method of treating airway disease in a subject in need of such treatment, comprising: topically administering an antisense oligonucleotide to the airway epithelium of said subject in an amount effective to treat said disease; said antisense oligonucleotide being essentially free of adenosine. 2. A method according to claim 1 wherein said airway disease is a lung disease and said airway epithelium is a lung airway epithelium. 3. A method according to claim 1 wherein said antisense oligonucleotide comprises nucleotides in which at least one phosphodiester linkage is replaced with a linkage selected from the group consisting of methylphosphonate linkages, phosphotriester linkages, phosphorothioate linkages, phosphorodithioate linkages, and phosphoramidate linkages. 4. A method according to claim 1 wherein said airway disease is selected from the group consisting of cystic fibrosis, asthma, chronic obstructive pulmonary disease, bronchitis, and other airway diseases characterized by an inflammatory response. 5. A method according to claim 1 wherein said antisense oligonucleotide is targeted against an mRNA encoding a protein selected from the group consisting of human A2a adenosine receptor, human A2b adenosine receptor, human IgE receptor β, human Fc-epsilon receptor CD23 antigen, human histidine decarboxylase, human beta tryptase, human tryptase-I, human prostaglandin D synthase, human cyclooxygenase-2, human eosinophil cationic protein, human eosinophil derived neurotoxin, human eosinophil peroxidase, human intercellular adhesion molecule-1 (ICAM-1) , human vascular cell adhesion molecule 1 (VCAM-1) , human endothelial leukocyte adhesion molecule (ELAM-1) , human P selectin, human endothelial monocyte activating factor, human IL-3, human IL-4, human IL-5, human IL-6, human IL-8, human monocyte-derived neutrophil chemotactic factor, human neutrophil elastase, human neutrophil oxidase factor, human cathepsin G, human defensin 1, human defensin 3, human macrophage inflammatory protein-1-alpha, human muscarinic acetylcholine receptor HM1, human muscarinic acetylcholine receptor HM3, human fibronectin, human GM- CSF, human tumor necrosis factor , human leukotriene C4 synthase, human major basic protein, and endothelin 1. 6. A method according to claim 1 wherein said antisense oligonucleotide is delivered by administering an aerosol of respirable particles containing said antisense oligonucleotide to the lungs of said subject. 7. A method according to claim 6, wherein said particles are selected from the group consisting of solid particles and liquid particles. 8. A method according to claim 6, wherein said aerosol is comprised of particles having a particle size within the range of about 0.5 to 10 microns. 9. A method according to claim 8 wherein said particles are liposomes containing said antisense oligonucleotide. 10. A method according to claim 6 wherein said antisense oligonucleotide is administered in amount sufficient to achieve intracellular concentrations of said antisense oligonucleotide in said subject from about 0.1 to 10 μM. 11. A pharmaceutical composition, comprising, together in a pharmaceutically acceptable carrier: an antisense oligonucleotide in an amount effective to treat an airway disease; said antisense oligonucleotide being essentially free of adenosine. 12. A pharmaceutical composition according to claim 11 wherein said airway disease is a lung disease and said airway epithelium is a lung airway epithelium. 13. A pharmaceutical composition according to claim 11 wherein said antisense oligonucleotide comprises nucleotides in which at least one phosphodiester linkage is replaced with a linkage selected from the group consisting of methylphosphonate linkages, phosphotriester linkages, phosphorothioate linkages, phosphorodithioate linkages, and phosphoramidate linkages. 14. A pharmaceutical composition according to claim 11 wherein said airway disease is cystic fibrosis. 15. A pharmaceutical composition according to claim 11 wherein said antisense oligonucleotide is targeted against an mRNA encoding a protein selected from the group consisting of human A2a adenosine receptor, human A2b adenosine receptor, human IgE receptor β , human Fc-epsilon receptor CD23 antigen, human histidine decarboxylase, human beta tryptase, human tryptase-I, human prostaglandin D synthase, human cyclooxygenase-2, human eosinophil cationic protein, human eosinophil derived neurotoxin, human eosinophil peroxidase, human intercellular adhesion molecule-1 (ICAM-1) , human vascular cell adhesion molecule 1 (VCAM-1) , human endothelial leukocyte adhesion molecule (ELAM-1) , human P selectin, human endothelial monocyte activating factor, human IL-3, human IL-4, human IL-5, human IL-6, human IL- 8, human monocyte-derived neutrophil chemotactic factor, human neutrophil elastase, human neutrophil oxidase factor, human cathepsin G, human defensin 1, human defensin 3, human macrophage inflammatory protein-1- alpha, human muscarinic acetylcholine receptor HM1, human muscarinic acetylcholine receptor HM3, human fibronectin, human GM-CSF, human tumor necrosis factor , human leukotriene C4 synthase, and human major basic protein. 16. A pharmaceutical composition according to claim 11 wherein said antisense oligonucleotide is delivered by administering an aerosol of respirable particles containing said antisense oligonucleotide to the lungs of said subject. 17. A pharmaceutical composition according to claim 16, wherein said particles are selected from the group consisting of solid particles and liquid particles. 18. A pharmaceutical composition according to claim 16, wherein said aerosol is comprised of particles having a particle size within the range of about 0.5 to 10 microns. 19. A pharmaceutical composition according to claim 16 wherein said particles are liposomes containing said antisense oligonucleotide. 20. A pharmaceutical composition according to claim 11 wherein said antisense oligonucleotide is administered in amount sufficient to achieve intracellular concentrations of said antisense oligonucleotide in said subject from about 0.1 to 10 μM. 21. A pharmaceutical composition according to claim 11, wherein said antisense oligonucleotide is conjugated to a molecule capable of cellular uptake.

Inventors: METZGER W JAMES, NYCE JONATHAN W, METZGER, W., JAMES, NYCE, JONATHAN, W.
Applicants: METZGER W JAMES, NYCE JONATHAN W, UNIV EAST CAROLINA, EAST CAROLINA UNIVERSITY, METZGER, W., JAMES, NYCE, JONATHAN, W.
Application year: 1996
Main classification: A61K31
IPC codes: A61K31, A61K38, A61K48, A61P11, C12N15, C12Q1