SUBSTRATES FOR BETA-LACTAMASE AND USES THEREOF

Abstract: Substrates for beta -lactamase of general formula (I) in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit); R' is selected from the group consisting of H, lower (i.e., alkyl of 1 to about 5 carbon atoms) and (CH2)nOH, in which n is 0 or an integer from 1 to 5; R" is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, -CHR<2>OCO(CH2)nCH3, -CHR<2>OCOC(CH3)3, acylthiomethyl, acyloxy-alpha-benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, acyloxyalkyl, dialkylaminocarbonyloxymethyl and aliphatic, in which R<2> is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO2 and CH2; and Z' and Z" are linkers for the fluorescent donor and quencher moieties. Methods of assaying beta -lactamase activity and monitoring expression in systems using beta -lactamase as a reporter gene also are disclosed.

Description: SUBSTRATES FOR BETA-LACTAMASE AND USES THEREOF BACKGROUND OP THE INVENTION The present invention relates generally to the fields of chemistry and biology. More particularly, the present invention relates to compositions and methods for use in measuring gene expression. A reporter gene assay measures the activity of a gene's promoter. It takes advantage of molecular biology techniques, which allow one to put heterologous genes under the control of any promoter and introduce the construct into the genome of a mammalian cell [Gorman, CM. et al., Mol . Cell Biol . 2 : 1044-1051 (1982) ; Alam, J. and Cook, J.L., J^nal . Bioche . 188 : 245-254, (1990)] . Activation of the promoter induces the reporter gene as well as or instead of the endogenous gene. By design the reporter gene codes for a protein that can easily be detected and measured. Commonly it is an enzyme that converts a commercially available substrate into a product. This conversion is conveniently followed by either chromatography or direct optical measurement and allows for the quantification of the amount of enzyme produced. Reporter genes are commercially available on a variety of plas ids for the study of gene regulation in a large variety of organisms [Alam and Cook, supra] . Promoters of interest can be inserted into multiple cloning sites provided for this purpose in front of the reporter gene on the plasmid [Rosenthal, N. , Methods Enzymol . 152 : 704-720 (1987) ; Shiau, A. and Smith, J.M. , Gene £7: 295-299 (1988)] . Standard techniques are used to introduce these genes into a cell type or whole organism [e.g., as described in Sambrook, J. , Fritsch, E.F. and Maniatis, T. Expression of cloned genes in cultured mammalian cells. In: Molecular Cloning, edited by Nolan, C. New York: Cold Spring Harbor Laboratory Press, 1989] . Resistance markers provided on the plasmid can then be used to select for successfully transfected cells. Ease of use and the large signal amplification make this technique increasingly popular in the study of gene regulation. Every step in the cascade DNA - -> RNA --> Enzyme - -> Product - -> Signal amplifies the next one in the sequence. The further down in the cascade one measures, the more signal one obtains. In an ideal reporter gene assay, the reporter gene under the control of the promoter of interest is transfected into cells, either transiently or stably. Receptor activation leads to a change in enzyme levels via transcriptional and translational events. The amount of enzyme present can be measured via its enzymatic action on a substrate. The substrate is a small uncharged molecule that, when added to the extracellular solution, can penetrate the plasma membrane to encounter the enzyme. A charged molecule can also be employed, but the charges need to be masked by groups that will be cleaved by endogenous cellular enzymes (e.g., esters cleaved by cytoplasmic esterases) . For a variety of reasons, the use of substrates which exhibit changes in their fluorescence spectra upon interaction with an enzyme are particularly desirable. In some assays, the fluorogenic substrate is converted to a fluorescent product. Alternatively, the fluorescent substrate changes fluorescence properties upon conversion at the reporter enzyme. The product should be very fluorescent to obtain maximal signal, and very polar, to stay trapped inside the cell. To achieve the highest possible sensitivity in a reporter assay one has to maximize the amount of signal generated by a single reporter enzyme. An optimal enzyme will convert 10 5 substrate molecules per second under saturating conditions [Stryer, L. Introduction to enzymes. In: Biochemistry, New York: W. H. Freeman and company, 1981, pp. 103-134] . 3-Lactamases will cleave about 10 3 molecules of their favorite substrates per second [Chang, Y.H. et al. , Proc . Natl . Acad. Sci . USA 8_7: 2823-2827 (1990)] . Using a fluorogenic substrate one can obtain up to 10 6 photons per fluorescent product produced, depending on the type of dye used, when exciting with light of the appropriate wavelength. The signal terminates with the bleaching of the fluorophore [Tsien, R.Y. and Waggoner, A.S. Fluorophores for confocal microscopy: Photophysics and photochemistry. In: Handbook of Biological Confocal Microscopy, edited by Pawley, J.B. Plenum Publishing Corporation, 1990, pp. 169-178] . These numbers illustrate the theoretical magnitude of signal obtainable in this type of measurement. In practice a minute fraction of the photons generated will be detected, but this holds true for fluorescence, bioluminescence or chemiluminescence. A good fluorogenic substrate for a reporter enzyme has to have a high turnover at the enzyme in addition to good optical properties such as high extinction and high fluorescence quantum yield. SUMMARY OF THE INVENTION It is an object of the present invention to provide jS-lactamase substrate compounds. It is a further object of the invention to provide membrane-permeant compounds. The membrane-permeant compounds may be transformed into substantially membrane-impermeant compounds. Another object of the invention is to provide β- lactamase reporter genes. A further object of the present invention is to create cells containing the β-lactamase reporter genes functionally linked to a promotor such that when the promotor is turned on, the reporter gene will be expressed. Expression of the β-lactamase is measured with the β-lactamase substrates which emit light after hydrolysis by the β -lactamase. A further object of the invention is to use the β- lactamase reporter genes in cells and the β-lactamase substrate compounds of the present invention to screen for biochemical activity. In accordance with the present invention, fluorogenic substrates are provided of the general formula I wherein: one of X and Y is a fluorescent donor moiety or a membrane-permeant derivative thereof, and the other is a quencher moiety, an acceptor fluorophore moiety or a membrane- permeant derivative thereof; R' is selected from the group consisting of H, lower alkyl, (CH 2 ) n 0H, (CH 2 ) n COOR", and =N0J, in which n is 0 or an integer from 1 to 5 and J is H, Me, CH 2 COOH, CHMeCOOH, and CMe 2 COOH; R" is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, - CHR 2 OCO(CH 2 ) n CH 3 , -CHR 2 OCOC (CH 3 ) 3 , acylthiomethyl, acyloxy-alpha- benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, betamorpholinoethyl, dialkylaminoethyl, dialkylaminocarbonyloxymethyl, in which R 2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, S0 2 and CH 2 ; Z' is a linker for X; and Z" is a linker for Y. In another aspect, this invention provides methods for determining whether a sample contains β-lactamase activity. The methods involve contacting the sample with a compound substrate of the invention, which exhibits fluorescence resonance energy transfer when the compound is excited; exciting the compound; and determining the degree of fluorescence resonance energy transfer in the sample. A degree of fluorescence resonance energy transfer that is lower than an expected amount indicates the presence of β-lactamase activity. One embodiment of this method is for determining the amount of an enzyme in a sample. According to this method, determining the degree of fluorescence resonance energy transfer in the sample comprises determining the degree at a first and second time after contacting the sample with the substrate, and determining the difference in the degree of fluorescence resonance energy transfer. The difference in the degree of fluorescence resonance energy transfer reflects the amount of enzyme in the sample. In another aspect, this invention provides recombinant nucleic acid molecule comprising expression control sequences adapted for function in a vertebrate cell and operably linked to a nucleotide sequence coding for the expression of a 0-lactamase. It also provides recombinant nucleic acid molecules comprising expression control sequences adapted for function in a eukaryotic cell and operably linked to a nucleotide sequence coding for the expression of a cytosolic / S-lactamase. In certain embodiments, the invention is directed to mammalian host cells transfected with these recombinant nucleic acid molecules. In another aspect, this invention provides methods for determining the amount of /.-lactamase activity in a cell. The methods involve providing a host cell transfected with a recombinant nucleic acid molecule comprising expression control sequences operatively linked to nucleic acid sequences coding for the expression of a β-lactamase; contacting a sample comprising the cell or an extract of the cell with a substrate for β-lactamase; and determining the amount of substrate cleaved, whereby the amount of substrate cleaved is related to the amount of β-lactamase activity. In another aspect, this invention provides methods for monitoring the expression of a gene operably linked to a set of expression control sequences. The methods involve providing a host cell transfected with a recombinant nucleic acid molecule comprising expression control sequences operatively linked to nucleic acid sequences coding for the expression of a / S-lactamase, except if the eukaryote is a fungus, wherein the β-lactamase is a cytosolic β-lactamase; contacting a sample comprising the cell or an extract of the cell or conditioned medium with a substrate for /3-lactamase; and determining the amount of substrate cleaved. The amount of substrate cleaved is related to the amount of /S-lactamase activity. In another aspect, this invention provides methods for determining whether a test compound alters the expression of a gene operably linked to a set of expression control sequences. The methods involve providing a cell transfected with a recombinant nucleic acid construct comprising the expression control sequences operably linked to nucleic acid sequences coding for the expression of a 3-lactamase except if the eukaryote is a fungus, wherein the /S-lactamase is a cytosolic /3-lactamase; contacting the cell with the test compound; contacting a sample comprising the cell or an extract of the cell with a / S-lactamase substrate; and determining the amount of substrate cleaved, whereby the amount of substrate cleaved is related to the amount of β- lactamase activity. In one embodiment of the methods, the substrate is a compound of this invention. The step of determining the amount of substrate cleaved comprises exciting the compound; and determining the degree of fluorescence resonance energy transfer in the sample. A degree of fluorescence resonance energy transfer that is lower than an expected amount indicates the presence of / S-lactamase activity. In another aspect, this invention provides methods of clonal selection comprising providing cells transfected with a recombinant nucleic acid molecule comprising the expression control sequences operably linked to nucleic acid sequences coding for the expression of a cytosolic β- lactamase; contacting the cells with a substance that activates or inhibits the activation of the expression control sequences; contacting the cells with a compound of claim 9 which is corverted into a substrate; determining whether substrate is cleaved within each individual cell, whereby cleavage reflects / S-lactamase activity; selecting and propagating those cells with a selected level of / S-lactamase activity. In a further embodiment, the method further involves culturing selected cells in the absence of activator for a time sufficient for cleaved substrate to be substantially lost from the cells and for / S-lactamse levels to return to unactivated levels; incubating the selected cells with a compound of claim 9 which is converted into a substrate; and selecting cells that have not substantially cleaved the substrate. BRIEF DESCRIPTION OF THE DRAWINGS Figs. 1(a) and 1(b) illustrate the emission spectra for the fluorescein (a) and rhodamine (b) components of compound 11 (Example 1) before and after β-lactamase cleavage of the β-lactam ring; Fig. 2 illustrates the emission spectrum of compound 17 before and after β-lactamase cleavage of the β-lactam ring; Fig. 3 illustrates the emission spectrum of compound 22 before and after β-lactamase cleavage of the β-lactam ring; Fig. 4 illustrates the emission spectrum of compound 25 before and after β-lactamase cleavage of the β-lactam ring; Fig. 5 illustrates the emission spectrum of compound CCF2 before and after β-lactamase cleavage of the β-lactam ring; and Fig. 6 illustrates the emission spectrum of compound CCF1 before and after β-lactamase cleavage of the β-lactam ring. Fig. 7A presents a table describing various nucleotide and amino acid sequences useful in the invention. Fig. 7B-C depicts Sequence 1, the nucleotide and deduced amino acid sequence of E. coli RTEM as modified by Kadonaga et al (1984) . Fig. 7D-E depicts Sequence 2, the nucleotide and deduced amino acid sequence of Wild-type secreted RTEM enzyme with Ser2→Arg, Ala23-»Gly. Fig. 7F-G depicts Sequence 3, the nucleotide and deduced amino acid sequence of RTEM enzyme with / S-globin upstream leader, mammalian Kozak sequence, replacement of signal sequence by Met Gly. Fig. 7H-I depicts Sequence 4, the nucleotide and deduced amino acid sequence of RTEM / S-lactamase with mammalian Kozak sequence and replacement of signal sequence by Met Asp. Fig. 7J-K depicts Sequence 5, the nucleotide and deduced amino acid sequence of Bacillus licheniformis β- lactamase with signal sequence replaced. DESCRIPTION OF THE INVENTION DEFINITIONS In accordance with the present invention and as used herein, the following terms are defined with the following meanings, unless stated otherwise. The term "fluorescent donor moiety" refers the radical of a fluorogenic compound which can absorb energy and is capable of transferring the energy to another fluorogenic molecule or part of a compound. Suitable donor fluorogenic molecules include, but are not limited to, coumarins and related dyes xanthene dyes such as fluoresceins, rhodols, and rhodamines, resorufins, cyanine dyes, bimanes, acridines, isoindoles, dansyl dyes, aminophthalic hydrazides such as luminol and isoluminol derivatives, aminophthalimides, aminonaphthalimides, aminobenzofurans, aminoquinolines, dicyanohydroquinones, and europium and terbium complexes and related compounds. The term "quencher" refers to a chromophoric molecule or part of a compound which is capable of reducing the emission from a fluorescent donor when attached to the donor. Quenching may occur by any of several mechanisms including fluorescence resonance energy transfer, photoinduced electron transfer, paramagnetic enhancement of intersystem crossing, Dexter exchange coupling, and exciton coupling such as the formation of dark complexes. The term "acceptor" as used herein refers to a quencher which operates via fluorescence resonance energy transfer. Many acceptors can re- emit the transferred energy as fluorescence. Examples include coumarins and related fluorophores, xanthenes such as fluoresceins, rhodols, and rhodamines, resorufins, cyanines, difluoroboradiazaindacenes, and phthalocyanines. Other chemical classes of acceptors generally do not re-emit the transferred energy. Examples include indigos, benzoquinones, anthraquinones, azo compounds, nitro compounds, indoanilines, di- and triphenylmethanes. The term "dye" refers to a molecule or part of a compound which absorbs specific frequencies of light, including but not limited to ultraviolet light. The terms "dye" and "chromophore" are synonymous. The term "fluorophore" refers to chromophore which fluoresces. The term "membrane-permeant derivative" means a chemical derivative of a compound of general formula wherein at least one of X and Y contains at least one acylated aromatic hydroxyl, acylated amine, or alkylated aromatic hydroxyl wherein the acyl group contains 1 to 5 carbon atoms and wherein the alkyl group is selected from the group consisting of -CH 2 OC(0)alk, -CH 2 SC(0)alk, -CH 2 0C(O)Oalk, lower acyloxy-alpha-benzyl, and deltabutyrolactonyl; wherein alk is lower alkyl of 1 to 4 carbon atoms. These derivatives are made better able to cross cell membranes, i.e. membrane permeant, because hydrophilic groups are masked to provide more hydrophobic derivatives. Also, the masking groups are designed to be cleaved from the fluorogenic substrate within the cell to generate the derived substrate intracellularly. Because the substrate is more hydrophilic than the membrane permeant derivative it is now trapped within the cells. The term "alkyl" refers to straight, branched, and cyclic aliphatic groups of 1 to 8 carbon atoms, preferably 1 to 6 carbon atoms, and most preferably 1 to 4 carbon atoms. The term "lower alkyl" refers to straight and branched chain alkyl groups of 1 to 4 carbon atoms. The term "aliphatic" refers to saturated and unsaturated alkyl groups of 1 to 10 carbon atoms, preferably 1 to 6 carbon atoms, and most preferably 1 to 4 carbon atoms. SUBSTRATES /8-Lactamases are nearly optimal enzymes in respect to their almost diffusion-controlled catalysis of / S-lactam hydrolysis [Christensen, H. et al. , Biochem . J. 266: 853-861 (1990)] . Upon examination of the other properties of this class of enzymes, it was determined that they were suited to the task of an intracellular reporter enzyme. They cleave the / S-lactam ring of /3-lactam antibiotics, such as penicillins and cephalosporins, generating new charged moieties in the process [0'Callaghan, CH. et al. , An timicrob . Agents . Chemother. .8: 57-63, (1968); Stratton, C.W., J. Antimicrob . Chemother. 22, SUPDI. A: 23-35 (1988)]. A first generation cephalosporin is illustrated below, left, with the arrow pointing to the site of cleavage by / S-lactamase. The free amino group thus generated (middle structure below) donates electron density through the vinyl group to promote irreversible cleavage of a nucleofugal group R 2 from the 3' -position. R 2 is thus free to diffuse away from the Ri-cephalosporin conjugate (right-hand structure below) . site of enzymatic cleavage /3-Lactamases are a class of enzymes that have been very well characterized because of their clinical relevance in making bacteria resistant to / S-lactam antibiotics [Waley, S.G., Sci . Prog. 12 : 579-597 (1988); Richmond, M.H. et al. , Ann . N. Y. Acad. Sci . 182: 243-257 (1971)] . Most / S-lactamases have been cloned and their amino acid sequence determined [see, e.g., Ambler, R.P., Phil . Trans . R . Soc . Lond . [Ser. B . ] 289: 321-331 (1980)] . A gene encoding .-lactamase is known to molecular biologists as the a picillin resistance gene (Amp r ) and is commonly used to select for successfully transduced bacteria [Castagnoli, L. et al., Genet. Res . 4.0: 217-231 (1982)]; clones thereof are almost universally available. The enzyme catalyzes the hydrolysis of a /3-lactam ring and will not accept peptides or protein substrates [Pratt, R.F. and Govardhan, C.P., Proc . Natl . Acad. Sci . USA 81 : 1302-1306 (1984); Murphy, B.P. and Pratt, R.F., Biochemistry 30: 3640-3649 (1991)] . The kinetics of this reaction is well understood and there is no product inhibition [Bush, K. and Sykes, R.B., AntimicroJ . Agents . Chemother. 2 : 6-10 (1986); Christensen et al. (1990), supra] . The enzyme substrates are less polar than the products. The carboxyl group in the substrate can be easily masked by an acetoxymethyl ester [Jansen, A.B.A. and Russell, T.J., J. Chem. Soc . 2127-2132, (1965); Daehne, W. et al. , J " .Med.Chem. 13: 607-612 (1970)], which is readily cleaved by endogenous mammalian intracellular esterases. Conversion by these esterases followed by the / S-lactam cleavage by / 3-lactamase generates two negative charges and a tertiary amine, which protonates. To date, there has been no report of a fluorogenic substrate with the appropriate properties, but multiple chromogenic substrates of different design have been reported and are commercially available [Jones, R.N. et al., J. Clin . Microbiol . 15.: 677-683 (1982); Jones, R.N. et al. , J\Clin.Λ.icroJiol. 15: 954-958 (1982); O'Callaghan, CH. et al., Ant i icrob. Agents . Chemother. 1 - 283-288 (1972)] . A large number of /3-lactamases have been isolated and characterized, all of which would be suitable for use in accordance with the present invention. Initially, /3-lactamases were divided into different classes (I through V) on the basis of their substrate and inhibitor profiles and their molecular weight [Richmond, M.H. and Sykes, R.B., Adv. Microb. Physiol . 9 : 31-88 (1973)] . More recently, a classification system based on amino acid and nucleotide sequence has been introduced [A bler, R.P., Phil . Trans . R . Soc . Lond. [Ser. B . ] 281: 321-331 (1980)] . Class A 3-lactamases possess a serine in the active site and have an approximate weight of 29kd. This class contains the plasmid- mediated TEM / 3-lactamases such as the RTEM enzyme of pBR322. Class B / S-lactamases have an active-site zinc bound to a cysteine residue . Class C enzymes have an active site serine and a molecular weight of approximately 39kd, but have no amino acid homology to the class A enzymes. The coding region of an exemplary β-lactamase employed in the reporter gene assays described herein is indicated in SEQ ID NO:l (nucleic acid sequence) and SEQ ID NO:2 (amino acid sequence) . The pTG2dell containing this sequence has been described [Kadonaga, J.T. et al . , J. Biol . Chem. 259: 2149-2154 (1984)] . The entire coding sequence of wildtype pBR322 β-lactamase has also been published [Sutcliffe, J.G., Proc . Natl . Acad. Sci . USA 75: 3737-3741 (1978)] . As would be readily apparent to those skilled in the field, this and other comparable sequences for peptides having β-lactamase activity would be equally suitable for use in accordance with the present invention. The β- lactamase reporter gene is employed in an assay system in a manner well known per se for the use of reporter genes (for example, in the form of a suitable plasmid vector) . In conjunction with a suitable β-lactamase, there are employed in accordance with the present invention fluorogenic substrates of the general formula I in which one of X and Y is a fluorescent donor moiety and the other is a quencher (which may or may not re-emit) ; R' is selected from the group consisting of H, lower alkyl, (CH 2 ) n 0H, (CH 2 ) n COOR", and =N0J, in which n is 0 or an integer from 1 to 5 and J is H, Me, CH 2 COOH, CHMeCOOH, and CMe 2 COOH; R" is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, -CHR 2 OCO (CH 2 ) n CH 3 , - CHR 2 OCOC(CH 3 ) 3 , acylthiomethyl, acyloxy-alpha-benzyl, delta- butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, beta-morpholinoethyl, dialkylaminoethyl, and dialkylaminocarbonyloxymethyl, in which R 2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, S0 2 and CH 2 ; and Z' and Z" are linkers for the fluorescent donor and quencher moieties. The linkers Z' and Z" serve the purpose of attaching the fluorescent donor and quencher moieties to the cephalosporin-derived backbone, and may facilitate the synthesis of the compounds of general formula I. In general formula I, Z' may represent a direct bond to the backbone; alternatively, suitable linkers for use as Z' include, but are not limited to, the following: - (CH 2 ) n C0NR 2 (CH 2 ) m - , - (CH 2 ) n NR 2 CO(CH 2 ) m -, - ( CH 2 ) n NR 3 CONR 2 ( CH 2 ) m - , - ( CH 2 ) n NR 3 CSNR 2 ( CH 2 ) m - , - ( CH 2 ) n CONR 3 ( CH 2 ) p CONR 2 ( CH 2 ) _- , - (CH 2 ) n - , -(CH 2 ) n NR 3 CO(CH 2 ) p S(CH 2 ) m - / - (CH 2 ) π S (CH 2 ) m - , - (CH 2 ) n O(CH 2 ) m -, -(CH 2 )_NR 2 (CH 2 ) m -, -(CH 2 ) n S0 2 NR (CH 2 ) m -, - (CH 2 ) n C0 2 (CH 2 ) m - , aanndd wherein R 2 and n are as previously defined; R 3 is selected from the group consisting of hydrogen and lower alkyl; and each of m and p is independently selected from the group consisting of 0 and integers from 1 to 4. Especially preferred are Z' groups such where n and m are 0. Also particularly preferred are such Z' groups where R 2 is H. Suitable linkers Z" for the Y moiety include, but are not limited to, a direct bond to a heteroatom (e.g., O, N or S) in the dye's chromophore or the following: -0(CH 2 ) n -, -S(CH 2 ) n -, -NR 2 (CH 2 ) n -, -N*R 2 (CH 2 ) n - , -OCONR 2 (CH 2 ) n - , -0 2 C(CH 2 ) n -, -SCSNR 2 (CH 2 ) n -, -SCSO(CH 2 ) n - , -S (CH 2 ) n CONR 2 (CH 2 ) m , -S(CH 2 ) n NR 2 CO(CH 2 ) m , and ^N(CH2) m - in which R 2 , n and m are as previously defined; and m is an integer from 0 to 4. Particularly preferred Z" groups are - S(CH,)_. Especially preferred is H. Preferred R' groups include H and methyl. Particularly preferred is H. Preferred R" groups include H and acetoxymethyl. A preferred R 2 group is H. A preferred A group is -S-. In a preferred aspect, the compounds of the present invention are membrane-permeant. Particularly preferred are such compounds wherein at least one of X and Y contains at least one acylated aromatic hydroxyl, acylated amine, or alkylated aromatic hydroxyl wherein the acyl group contains 1 to 5 carbon atoms and wherein the alkyl group is selected from the group consisting of -CH 2 0C(0)alk, -CH 2 SC(O)alk, - CH 2 OC(0)Oalk, lower acyloxy-alpha-benzyl, and delta¬ butyrolactonyl, wherein alk is lower alkyl of 1 to 4 carbon atoms. Particularly preferred are such compounds where at least one of X and Y contains at least one acylated aromatic hydroxy, wherein the acyl group is either acetyl, n-propionyl, or n-butyryl. Also particularly preferred are such compounds wherein at least one of X and Y contains an acetoxy methyl group on an aromatic hydroxyl group. In another preferred aspect, the quencher or acceptor is a fluorescein, rhodol, or rhodamin of formulae VIII-XII. Preferred are such compounds where the donor is a fluorescein of formula VIII and the quencher or acceptor is a rhodol or rhodamine of formulae VIII-XII. Also preferred are such compounds where the donor is a fluorescein of formula VIII and the quencher or acceptor is a tetrahalo fluorescein of formula VIII in which R a , R b , R c , and R d are independently Br or Cl. Also preferred are such compounds where the quencher or acceptor is a rhodol of formulae VIII, IX, and XI. Another preferred group of such compounds are those where the quencher or acceptor is a rhodamine of formulae VIII, X, and XII. In a another preferred aspect, the donor is a coumarin of formulae II-VII and the quencher/acceptor is a fluorescein, rhodol, or rhodamine of formulae VIII-XII, XLVII, or XLVII, and membrane-permeant fluorogenic derivatives thereof. Particularly preferred are such compounds with a fluorescein quencher/acceptor of formula VIII. Especially preferred are such compounds where the coumarin is 7- hydroxycoumarin or 7-hydroxy-6-chlorocoumarin and the fluorescein acceptor is fluorescein or dichlorofluorescein. As would readily be appreciated by those skilled in the art, the efficiency of fluorescence resonance energy transfer depends on the fluorescence quantum yield of the donor fluorophore, the donor-acceptor distance and the overlap integral of donor fluorescence emission and acceptor absorption. The energy transfer is most efficient when a donor fluorophore with high fluorescence quantum yield (preferably, one approaching 100%) is paired with an acceptor with a large extinction coefficient at wavelengths coinciding with the emission of the donor. The dependence of fluorescence energy transfer on the above parameters has been reported [Forster, T. (1948) Ann. Physik 2 : 55-75; Lakowicz, J.R., Principles of Fluorescence Spectroscopy, New York:Plenum Press (1983); Herman, B., Resonance energy transfer microscopy, in: Fluorescence Microscopy of Living Cells in Cul ture, Part B, Methods in Cell Biology, Vol 30, ed. Taylor, D.L. & Wang, Y.-L., San Diego: Academic Press (1989), pp. 219- 243; Turro, N.J., Modern Molecular Photochemistry, Menlo Part: Benjamin/Cu mings Publishing Co., Inc. (1978), pp. 296-361], and tables of spectral overlap integrals are readily available to those working in the field [for example, Berlman, I.B. Energy transfer parameters of aromatic compounds, Academic Press, New York and London (1973)] . The distance between donor fluorophore and acceptor dye at which fluorescence resonance energy transfer (FRET) occurs with 50% efficiency is termed R 0 and can be calculated from the spectral overlap integrals. For the donor-acceptor pair fluorescein - tetramethyl rhodamine which is frequently used for distance measurement in proteins, this distance R 0 is around 50-70 A [dos Remedios, C.G. et al. (1987) J. Muscle Research and Cell Motili ty 8:97-117] . The distance at which the energy transfer in this pair exceeds 90% is about 45 A. When attached to the cephalosporin backbone the distances between donors and acceptors are in the range of 10 A to 20 A, depending on the linkers used and the size of the chromophores. For a distance of 20 A, a chromophore pair will have to have a calculated R 0 of larger than 30 A for 90% of the donors to transfer their energy to the acceptor, resulting in better than 90% quenching of the donor fluorescence. Cleavage of such a cephalosporin by / S-lactamase relieves quenching and produces an increase in donor fluorescence efficiency in excess of tenfold. Accordingly, it is apparent that identification of appropriate donor-acceptor pairs for use as taught herein in accordance with the present invention would be essentially routine to one skilled in the art. To measure β-lactamase activity in the cytoplasm of living cells, smaller molecular weight chromophores as hereinafter described are in general preferred over larger ones as substrate delivery becomes a problem for larger compounds. Large molecules, especially those over about 1200 daltons, also tend to bind more avidly to cellular constituents than small ones, thereby removing at least some of them from access and cleavage by β-lactamase. Chromophores suitable for use as X and Y are well known to those skilled in the art. Generic structures of particular classes of chromophores suitable for use as X and Y are provided below. Compounds of general formulas II - XXXIV are exemplary of fluorophores which serve as the basis for particularly suitable donor moieties in the compounds of general formula I. Suitable chromophores for use as the basis of acceptor moieties in the compounds of general formula include, but are not limited to, compounds of general formulas II - LIV. Chromophores of general formulae XXXV-LIV usually do not re-emit efficiently. Coumarins and related dves π m IV V IV VII Xanthene dves .including fluorescein-. rhodols and rhodamines . Re XIV Difluoroboradiazaindacene dves Bimanes XIX xvm Acridines XX XXI xxπ Ispindoles xxm XXIV Dansvl dves R π XXV XXVI Aminophthalic hvrirazides {\i ~ ni ~ -\ ~~r isoliiminnl derivatives) XXVII XXVIII Amiπnn t-ialimides XXIX XXX .Λminonaphthalimides Aminohenzo-urans Aminoquinolines Dicvanohvdrnquinnnes xxxra XXXIV Indigo dves ftηf.ιraφιiτιnne dves XXXVI Pnlvmethine dves xxxvπ xxxvm xxxix Nitro dves and rvann derivatives XL XLI XLΠ XLΠI Quiπoπe dyes XLIV XLV Xanthene dves Dicvannvinvl and tricvannvinvl dves RflRhN CN XL VIII XLIX LI Indoaniiine dves .ninhvdrin derivatives . Di- and triohenvlmethane dves RflRh In preferred embodiments of the compounds of general formulas II - LVI: each of a and a' is independently H or an attachment point (i.e., a location at which the dye moiety is attached to the core structure of general formula I; E is selected from the group consisting of H, OH, OR" and NR 9 R h ; G is selected from the group consisting of 0 and N*R 9' R h '; each of L and L' is independently selected from the group consisting of CH and N; M is selected from the group consisting of H, Mg, Al, Si, Zn, and Cu; Q is selected from the group consisting of 0, S, C(CH 3 ) 2 and NR 9 ; Q' is selected from the group consisting of 0, CH 2 , C(CH 3 ) 2 , NR k and S0 2 ; T is selected from the group consisting of 0 and NR*; each of and W is selected from the group consisting of O, S, Se and NH; each of R a , R b , R c and R d is independently selected from the group consisting of an attachment point, H, halogen and lower alkyl; R e is selected from the group consisting of an attachment point, H, lower alkyl, (CH 2 ) n C0 2 H, (CH 2 ) n CHaC0 2 H, CHa(CH 2 ) n C0 2 H, (CH 2 ) n C0a, CH=CHCOa, each of R f , R 9 , R 9' , R h , R h' and R is independently selected from the group consisting of an attachment point, H, lower alkyl and CH 2 (CH 2 ) n a; R 1 is selected from the group consisting of an attachment point, H, halogen, lower alkyl, CN, CF 3 , phenyl, C0 2 H and CONR 9 'R h '; R j is selected from the group consisting of an attachment point, H, halogen, lower alkyl, CN, CF 3 , phenyl, CH 2 C0 2 H, CH 2 C0NR 9 'R h' ; each of R 1 and R r is independently selected from the group consisting of an attachment point, H, lower alkyl, each of R m , R n , R p and R q is independently selected from the group consisting of an attachment point, H, lower alkyl and phenyl; R° is selected from the group consisting of an attachment point, H and lower alkyl; each of R 3 and R c is independently selected from the group consisting of an attachment point, H, halogen, lower alkyl and OR f ; each of R u and R v is independently selected from the group consisting of an attachment point, H, halogen, CN and N0 2 ; each of R w is independently selected from the group consisting of an attachment point, H, COO " , S0 3 " , and P0 3 2" Ln is selected from the group consisting of Eu 3+ , Ln 3 \ and Sm 3+ ; Chel is a polydentate chelator with at least six and preferably eight to ten donor atoms that can face into a cavity of diameter between 4 and 6 angstroms, which may or may not be macrocyclic, which includes a chromophore absorbing between 300 and 400 nm, and which includes an attachment point through which Chel can be conjugated to Z' or Z" . A suitable Chel moiety is a europium tris- (bipyridine) cryptands. In the anthraquinone chromophores of general formula XXXIX, each of positions 1-8 may carry a substituent H or E, or serve as an attachment point. Europium tris- (bipyridine)cryptand donors may be suitably paired with acceptors of the formulae XV-XVII, XXXVI, XLVI-XLVII, LIV, and LVI. Terbium tris- (bipyridine) cryptand donors may be suitably paired with acceptors of the formulae VIII-XVIII. XXXVI-XLI, and XLV-LIV, and LVI. The Europium tris- (bipyridine) cryptand/ phtalocyanines donor/acceptor pair may be of particular interest when it is desirable to measure jβ-lactamase activity by emission of energy in the near to far red range. In many applications it is desirable to derivatize compounds of general formula I to render them hydrophobic and permeable through cell membranes. The derivatizing groups should undergo hydrolysis inside cells to regenerate the compounds of general formula I and trap them inside the cells. For this purpose, it is preferred that any phenolic hydrox'^s or free amines in the dye structures are acylated with C 1 -C 4 acyl groups (e.g. formyl, acetyl, n-butryl) or converted to various other esters and carbonates [for examples, as described in Bundgaard, H., Design of Prodrucrs, Elsevier Science Publishers (1985), Chapter I, page 3 et seq.] . Phenols can also be alkylated with 1- (acyloxy)alkyl, acylthiomethyl, acyloxy-alpha-benzyl, deltabutyrolactonyl, or methoxycarbonyloxymethyl groups. In the case of fluoresceins, rhodols, and rhodamines this manipulation is particularly useful, as it also results in conversion of the acid moiety in these dyes to the spirolactone. To promote membrane permeation, the carboxyl at the 4-position of the cephalosporin should be esterified with 1- (acyloxy)alkyl, acylthiomethyl, acyloxy-alpha-benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl ,m ethylsulfinylmethyl, beta- morpholionethyl, 2- (dimethylamino)ethyl, 2- (diethylamino)ethyl, or dialkylaminocarbonyloxymethyl groups as discussed in Ferres, H. (1980) Chem . Ind. 1980: 435-440. The most preferred esterifying group for the carboxyl is acetoxy ethyl. A general method for synthesis of compounds of general formula I is depicted below. As one of ordinary skill in the art will appreciate, the methods below can be used for a variety of derivatives, and other methods of synthesis are possible. Dye θ Hal. + J ^ + J→l -G + uo + RG' In these compounds, RG is a nucleophile-reactive group (e.g., iodoacetamide, isocyanate, isothiocyanate, etc.); Nu is a nucleophile (e.g., -SH, -NH 2 , -OH, etc.); R 0 is H or an ester group (e.g., benzhydryl ester, tertiary butyl ester, etc.); Nu 0 is a bidentate nucleophile (e.g., HS " , HSCH 2 CH 2 NH 2 , xanthate, etc.); and Hal is a halogen (e.g., chlorine, bromine or iodine) . The cephalosporin starting materials are commercially available cephalosporin derivatives 7- aminocephalosporanic acid or 7-amino 3'-chloro- cephalosporanic acid as its benzhydryl or tertiary butyl ester (R 0 ) . Prior to coupling the dyes A and B carrying nucleophile reactive groups (RG) it is sometimes advantageous to esterify or alkylate their phenolic and free amine residues. The order of attaching dye A and dye B depends on the choice of reagents. Dye A is tethered to the cephalosporin via an alkyl amide linker. This is achieved by reacting a dye A carrying a nucleophile-reactive group (RG) with a bifunctional aliphatic acid (e.g., amino-, mercapto- or hydroxyalkyl acid) and coupling of the acid to the cephalosporin 7-amine (path 1) . Alternatively, dye A carrying a nucleophilic group (e.g., amine or thiol) is reacted with a halogenated alkyl acid and the acid coupled to the cephalosporin 7-amine (path 2) . In both pathways, the order of the two reactions can be reversed. Dyes A containing an aliphatic acid can be directly coupled to the cephalosporin (path 3) . Dye B carrying a nucleophilic substituent can be coupled to the 3'-position in the cephalosporin by direct displacement of the leaving group (LG) (path 4) . A Dye B carrying a nucleophile-reactive group can be reacted with a bidentate nucleophile which is coupled then attached to the cephalosporin by leaving group (LG) displacement (path 5) ; the order of the reactions can be reversed. In some cases it might be necessary to conduct tne first reaction with a bidentate nucleophile with one of its nucleophilic groups masked. The second coupling is then performed after removal of that protection group. After attachment of both dyes the cephalosporin ester is cleaved (in cases where R 0 is not H) . To make membrane permeant substrates the acid is then re-esterified to esters that can be deprotected by the cytoplasmic environment of a mammalian cell. For applications not involving cell cytoplasm, any remaining acyl and alkyl groups that were used to mask phenols and free amines on the dyes are removed. Preferred combinations of classes of donors and acceptors suitable for use in accordance with the present invention are indicated in Table 1. In embodiments of compounds of general formula I using these combinations, fluorescent resonant energy transfer (FRET) occurs. Of course, as would be readily understood by those working in the field, many other combinations of donors and acceptors/quenchers (including those that re-emit and those that do not) would be suitable for use in accordance with the present invention. In general, suitable donor and acceptor pairs are those where the donor's emission spectrum signi icantly overlaps the acceptor's excitation spectrum. TABLE 1 DONORS II - VIII, XIX VII - XIV, XV - XVI, LV - XXI, XXIII - XVII, XXII ACCEPTORS XXXIV II - VIII, XIX FRET - XXI, XXIII - XXXIV VII - XIV, FRET FRET XVII, XXII XV - XVII FRET FRET FRET XL - XLV, FRET FRET XLVII - LII XXXV-XXXIX, FRET FRET FRET XLVI-XLVII, LIII-LIV, LVI Fluorescent donor moieties of particular interest include coumarins and fluoresceins. Particular quenchers of interest include fluoresceins, rhodols and rhodamines. Combinations of interest include the use of a coumarin donor with a fluorescein, rhodol or rhodamine quencher, and a fluorescein donor with a rhodol or rhodamine quencher. Specific combinations of interest include the following: a coumarin (e.g., 7-hydroxycouma in) or chloro derivative thereof with a fluorescein or dichloro derivative thereof; a fluorescein with an eosin or tetrachlorofluorescein; a fluorescein with a rhodol derivative; and a rhodamine with a fluorescein. Europium chelate donors may be suitably paired with acceptors of the formulae XV-XVII, XXXVI, XLVI-XLVII, LIV, and LVI. Terbium chelate donors may be suitably paired with acceptors of the formulae VIII-XVIII, XXXVI-XLI, and XLV-LIV, and LVI. The europium and terbium chelate donors may be of particular interest for their very narrow emission peaks and their microsecond-to-millisecond excited state lifetimes, which can be readily discriminated from background fluorescence and scattering with excited-state lifetimes of nanoseconds or less. donor fluorescence is quenched. In the case of the acceptor being itself a fluorescent dye, fluorescence re-emission occurs at the acceptor's emission wavelength. In polar solvents such as water, hydrophobic donor and acceptor fluorophores can stack when separated by a short flexible linker. Due to this association in the ground state, a "dark complex" is formed [Yaron, A. et al., Anal. Biochem. 95: 228-235 (1979)]. In this complex, neither fluorophore can emit light, causing the fluorescence of both dyes to be quenched [Bojarski, C. and Sienicki, K. Energy transfer and migration in fluorescent solutions. In: Photochemistry and Photophysics, edited by Rabek, J.F. Boca Raton: CRC Press, Inc., 1990, pp. 1-57] . In either case, a large change in fluorescence goes along with 3-lactam cleavage, which can be used to measure /3-lactamase activity. As both dyes diffuse away from each other, stacking and energy transfer are disrupted. Cephalosporins carrying a donor and an acceptor dye which fluoresces are referred to herein as FRET- cephalosporins. Fluorescence resonance energy transfer has been used as a spectroscopic ruler for measuring molecular distances in proteins and peptides as it is effective in the range from 10-lOOA. This energy transfer is proportional to the inverse sixth power of the distance between donor and acceptor. Its efficiency is higher, the better donor emission and acceptor absorbance overlap, and the longer the fluorescence lifetime of the donor (in absence of the acceptor) . FRET can be very efficient over distances of 10-20A. In the cephalosporin, distances for attachment of donor and acceptor are greater than lOA and a minimum of 10 bond-lengths, if one includes the two minimal spacers at 7- and 3-positions. Over this distance FRET is very efficient, if the right donor-acceptor pairs are chosen. Conveniently, in a FRET-cephalosporin the 7-amine tethered dye stays attached to the polar hydrolysis products of cephalosporin cleavage, trapping it in the cells' cytoplasm. This position s best occupied by the donor fluorophore, although in some αstances he acceptor may occupy this position. Upon chilling. The color change (even in individual small mammalian cells) is visible through a fluorescence microscope using normal color vision or photographic film; the fluorescence signal may be quantified and further enhanced by conventional digital image processing techniques. Moreover, because gene activation is detected not by a change in a single intensity but rather by a color change or a change in the ratio between two intensities at different wavelengths, the assays of the present invention are relatively immune to many artifacts such as variable leakiness of cells, quantity of substrate, illumination intensity, absolute sensitivity of detection and bleaching of the dyes. A variety of substrates (e.g., compounds of geneial formulas 17, 22 and 25) have been prepared and their emission spectra obtained before and after β-lactamase cleavage. These substrates allow for β-lactamase detection primarily in vi tro, as they bind strongly to serum and cellular proteins. Due to their hydrophobic nature, the fluorophores stack; this leads to a loss of fluorescence in the intact substrate. β- lactamase cleaves the substrates and relieves the stacking, allowing for fluorescence. Compounds (e.g., compound 11, Example 1) with reversed location of donor and acceptor fluorophore on the cephalosporin exhibit similar fluorescence behavior. In one preferred embodiment of the invention, a compound of general formula 1 was coupled to a compound of general formula 2 to form a compound of general formula 3. Commercially-available compound 4 was then coupled to compound 3 using dicyclohexylcarbodiimide and the product reacted with compound 5, yielding a compound of general formula 6. Deprotection of compound 6 generated a compound of general formula 7. In exemplary embodiments, Acyl was acetyl, R was Me and R y H (a) , or Acyl was butyryl, R was H and R y Cl (b) ; R z was trimethylsilyl or benzyl. 1 a,b 2a,b 1. coupling 2. deprotect acid 6a,b 20 deprotect 7a,b 30 The compounds of general formula 6 were modified to obtain membrane permeant derivatives which were converted to the corresponding fluorescent compounds of general formula 7 in intact cells due to the action of endogenous nonspecific esterases. In these molecules, fluorescence resonance energy transfer occurs from the 7-hydroxycoumarin moiety to the fluorescein moiety, leading to green fluorescence when the compounds are excited at about 400 nm. After cleavage of the β-lactam ring, excitation of the 7-hydroxycoumarin moiety results in blue fluorescence; in exemplary embodiments, a 25- fold increase in fluorescence at about 450 nm and a three- to fourfold decrease in fluorescence at 515 nm was observed. MONITORING GENE EXPRESSION The substrates of this invention make it feasible to use / S-lactamase as a reporter gene to monitor the expression from a set of expression control sequences. In one aspect, this invention provides methods for monitoring gene expression from a set of expression control sequences by using β- lactamase as a reporter gene. A cell is provided that has been transfected with a recombinant nucleic acid molecule comprising the expression control sequences operably linked to nucleic acid sequences coding for the expression of β- lactamase. Recombinant Nucleic Acids As used herein, the term "nucleic acid molecule" includes both DNA and RNA molecules. It will be understood that when a nucleic acid molecule is said to have a DNA sequence, this also includes RNA molecules having the corresponding RNA sequence in which "U" replaces "T." The term "recombinant nucleic acid molecule" refers to a nucleic acid molecule which is not naturally occurring, and which comprises two nucleotide sequences which are not naturally joined together. Recombinant nucleic acid molecules are produced by artificial combination, e.g., genetic engineering techniques or chemical synthesis. Nucleic acids encoding 3-lactamases can be obtained by methods known in the art, for example, by polymerase chain reaction of cDNA using primers based on the DNA sequence in Fig. 1. PCR methods are described in, for example, U.S. Pat. No. 4,683,195; Mullis et al. (1987) Cold Spring Harbor Sy p . Quant . Biol . 51:263; and Erlich, ed. , PCR Technology, (Stockton Press, NY, 1989) . The construction of expression vectors and the expression of genes in transfected cells involves the use of molecular cloning techniques also well known in the art. Sambrook et al . , Molecular Cloning -- A Laboratory Manual , Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, (1989) and Current Protocols in Molecular Biology, F.M. Ausubel et al. , eds., (Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (most recent Supplement) ) . Nucleic acids used to transfect cells with sequences coding for expression of the polypeptide of interest generally will be in the form of an expression vector including expression control sequences operatively linked to a nucleotide sequence coding for expression of the polypeptide. As used, the term nucleotide sequence "coding for expression of" a polypeptide refers to a sequence that, upon transcription and translation of mRNA, produces the polypeptide. As any person skilled in the are recognizes, this includes all degenerate nucleic acid sequences encoding the same amino acid sequence. This can include sequences containing, e.g., introns. As used herein, the term "expression control sequences" refers to nucleic acid sequences that regulate the expression of a nucleic acid sequence to which it is operatively linked. Expression control sequences are "operatively linked" to a nucleic acid sequence when the expression control sequences control and regulate the transcription and, as appropriate, translation of the nucleic acid sequence. Thus, expression control sequences can include appropriate promoters, enhancers, transcription terminators, a start codon (i.e., ATG) in front of a protein- encoding gene, splicing signals for introns, maintenance of SUL j r.TESHEET(RULE 26) the correct reading frame of that gene to permit proper translation of the mRNA, and stop codons. The recombinant nucleic acid can be incorporated into an expression vector comprising expression control sequences operatively linked to the recombinant nucleic acid. The expression vector can be adapted for function in prokaryotes or eukaryotes by inclusion of appropriate promoters, replication sequences, markers, etc. The recombinant nucleic acid used to transfect the cell contains expression control sequences operably linked to a nucleotide sequence encoding a /3-lactamase. The / 3-lactamase encoded can be any known to the art or described herein. This includes, for example, the enzymes shown in Fig. 7. This invention provides novel recombinant nucleic acid molecules including expression control sequences adapted for function in a non-mammalian eukaryotic cell operably linked to a nucleotide sequence coding for the expression of a cytosolic /3-lactamase. As used herein, "cytosolic β- lactamase" refers to a / 3-lactamase that lacks amino acid sequences for secretion from the cell membrane, e.g., the signal sequence. For example, in the polypeptide of Sequence 1 of Fig. 7, the signal sequence has been replaced with the amino acids Met-Ser. Accordingly, upon expression, this β- lactamase remains within the cell. This invention provides recombinant nucleic acid molecules including expression control sequences adapted for function in a mammalian eukaryotic cell operably linked to a nucleotide sequence coding for the expression of a β- lactamase. It is further preferable that the ribosome binding site and nucleotide sequence coding for expression of β - lactamase contain sequences preferred by mammalian cells. Such sequences improve expression of /3-lactamase in mammalian cells. Preferred sequences for expression in mammalian cells are described in, for example, Kozak, M., J. Cell Biol . 108 : 229-241 (1989), referred to herein as "Kozak sequences". The nucleotide sequence for cytosolic / 3-lactamase in Sequence 3 of SUBSTITUTESHEET(RULE2β) Fig. 7 contains Kozak sequences for the nucleotides -9 to 4 (GGTACCACCATGA) . When used in mammalian cells, the expression control sequences are adapted for function in mammalian cells. The method of this invention is useful to testing expression from any desired set of expression control sequences. In particular, this invention is useful for testing expression from inducible expression control sequences. As used herein, "inducible expression control sequences" refers to expression control sequences which respond to biochemical signals either by increasing or decreasing the expression of sequences to which they are operably linked. For example, in the case of genes induced by steroid hormones, the expression control sequences includes hormone response elements. The binding of a steroid hormone receptor to the response element induces transcription of the gene operably linked to these expression control sequences. Expression control sequences for many genes and for inducible genes, in particular, have been isolated and are well known in the art. The invention also is useful with constitutively active expression control sequences. The transfected cell is incubated under conditions to be tested for expression of / 3-lactamase from the expression control sequences. The cell or an extract of the cell is contacted with a /3-lactamase substrate of the invention under selected test conditions and for a period of time to allow catalysis of the substrate by any / 3-lactamase expressed. Then the donor moiety from this sample is excited with appropriate ultraviolet or visible wavelengths. The degree of fluorescence resonance energy transfer in the sample is measured. If the cell did not express /3-lactamase, very little of the substrate will have been cleaved, the efficiency of FRET in the cell will be high, and the fluorescence characteristics of the cell or sample from it will reflect this efficiency. If the cell expressed a large amount of β- lactamase, most of the substrate will be cleaved. In this case, the efficiency of FRET is low, reflecting a large amount CO CD CO CO m ro CD The expression control sequences also can be those responsive to substances that modulate cell-surface receptors or that modulate intra-cellular receptors. To measure whether a substance or mixture of substances activates extracellular or intracellular receptors or other cellular responses, cells containing beta-lactamase controlled by a desired promoter/enhancer element are incubated with test substance(s) , substrate then added, and after a certain period of time the fluorescence signal is measured at either one or two excitation-emission pairs appropriate to the chosen compound of the invention (e.g. compound CCF2 with wavelength pairs of near 405 nm and near 450 nm and near 405 and near 510 nm) . This fluorescent result is compared to control samples which have had no drug treatment and, when feasible, control samples with a known inhibitor and a known activator. The effect of any active drugs is then determined using the ratio of the fluorescence signal found in test wells to the signals found in wells with no drug treatment. Assays are performed in wells in a microtiter plate containing 96 or more wells or in an assay system with no compartments such as a gel matrix or moist membrane environment. Detection could be done for example by microtiter plate fluorimeters, e.g. Millipore Cytofluor, or imaging devices capable of analyzing one or more wells or one or more assay points in a certain surface area, e.g. as supplied by Astromed. The ability to retain the substrate in the cytoplasm of living cells is advantageous as it can allow a reduction in signal interference from coloured or quenching substances in the assay medium. Furthermore, the fluorescent signal from the compounds of this invention, such as CCF2, can be readily detected in single cells and thus allowing assay miniaturization and an increased number of tests per surface area. Miniaturized assays also further increase the throughput of an imaging detection system as there are more samples within the imaging field. The assay systems of the present invention further provide an advantageous and rapid method of isolation and clonal selection of stably transfected cell lines containing Additional suitable Chel moieties are described in Vallarino, L.M., & Leif, R.C., US Pat. 5,373,093; Sabbatini, N. et al , Pure and Applied Chem . 67: 135-140 (1995); Mathis, G., Clinical Chem. 41 : 1391-1397 (1995); Horiguchi, D., Chem . Pharm . Bull . 42: 972-975 (1994); Takalo, H. et al , Biocon juga te Chem. 5 278-282 (1994); Saha, A.K. eϋ al, J. A er . Chem . Soc. 115 11032 (1993); Li, M. & Selvin, P.R., J. Amer. Chem . Soc. 117 8132-8138 (1995) . Fluorescence in a sample is measured using a fluorimeter. In general, excitation radiation, from an excitation source having a first wavelength, passes through excitation optics. The excitation optics cause the excitation radiation to excite the sample. In response, fluorescent proteins in the sample emit radiation which has a wavelength that is different from the excitation wavelength. Collection optics then collect the emission from the sample. The device can include a temperature controller to maintain the sample at a specific temperature while it is being scanned. According to one embodiment, a multi-axis translation stage moves a microtiter plate holding a plurality of samples in order to position different wells to be exposed. The multi-axis translation stage, temperature controller, auto-focusing feature, and electronics associated with imaging and data collection can be managed by an appropriately programmed digital computer. The computer also can transform the data collected during the assay into another format for presentation. Methods of performing assays on fluorescent materials are well known in the art and are described in, e.g., Lakowicz, J.R., Principles of Fluorescence Spectroscopy, New York:Plenum Press (1983); Herman, B., Resonance energy transfer microscopy, in: Fluorescence Microscopy of Living Cells in Cul ture, Part B, Methods in Cell Biology, vol. 30, ed. Taylor, D.L. & Wang, Y.-L., San Diego: Academic Press (1989), pp. 219-243; Turro, N.J., Modern Molecular Photochemistry, Menlo Park: Benjamin/Cummings Publishing Col, Inc. (1978) , pp. 296-361. EXAMPLES All silica gel chromatography was performed using silica gel (Merck, grade 60, 230-400 mesh, 6θA) purchased from Aldrich. Bakerbond Octadecyl from J. T. Baker was used for C lβ reverse phase chromatography. Solvents (high pressure liquid chromatography grade) were used for chromatography as received, or dried over activated molecular sieves (3 A) for synthetic purposes. Fluorescence excitation and emission spectra were measured either on a Spex Fluorolog 111 or on a K2 fluorometer (ISS, Champaigne, ID in ratio mode with a rhodamine B quantum counter. The efficiency of fluorescence energy transfer was determined from the change in the integrated fluorescence emission at the donor emission wavelength upon treatment with β-lactamase. For fluorescence microscopy imaging, two different imaging setups were used. One, with an inverted fluorescence microscope, Zeiss IM-35 (Thornwood, NY) coupled to a silicon-intensified target (SIT) camera (Dage-MTI, Michigan City, IN) has been described in detail [Tsien, R.Y. (1986) New tetracarboxylate chelators for fluorescence measurement and photochemical manipulation of cytosolic free calcium concentrations, in: Optical Methods in Cell Physiology, ed. de Weer, P. & Salzberg, B., New York:Wiley, pp. 327-345; Tsien and Harootunian (1990) Cell Calcium 11:93- 109] . The other consisted of a cooled charge-coupled-device (CCD) camera (Photometries, Tucson, AZ) connected to an inverted fluorescence microscope (Zeiss Axiovert) . Fluorescence resonance energy transfer was measured by monitoring the ratio of fluorescence intensities at donor and acceptor emission wavelengths using commercially-available filters (Omega Optical) . Excitation: 360 DF 40, dichroic mirror 390 DCLP or 405 DF 15, dichroic mirror 420 DRLP02 Emission: 450 DF 65 (donor emission) 515 EFLP (acceptor emission) 435 EFLP (to view donor and acceptor fluorescence simultaneously) Example 1 (Compound 11) To test the optical properties of a cephalosporin with two dye molecules attached the following model compound was synthesized. The first synthesis step was to convert 7-aminocephalosporanic acid into a bifunctional cephalosporin carrying a thiol in the 3 ' -position and the 7-amine [Van Heyningen, E. and Brown, C.N., J.Wed.Chejn. 8.: 174-181 (1965); Japanese Patent, Kokai 75/18494, CA £ , 97320d] . This cephalosporin was then reacted selectively with an thiol-reactive dye, followed by an amine- reactive dye. The thiol-reactive dye 5, (6) -iodoacetamido- fluorescein and the amine-reac ive dye 5, (6) -carboxy- N,N,N' ,N' -tetramethylrhodamine-succinimide were coupled to the cephalosporin in aqueous dimethylformamide at pH 8. The product will be referred to as RCF. In phosphate buffer at pH 7 RCF is virtually non fluorescent; neither fluorescein nor rhodamine show much fluorescence when excited at their respective excitation maxima, which is indicative of chromophore stacking ("dark complex") . After long term treatment with /S-lactamase the /3-lactam is cleaved causing the fluorescence of both dyes to reappear (Figs. 1(a) and Kb)) . This experiment confirms that one can measure / S-lactamase catalyzed hydrolysis of the /3-lactam in cephalosporins by the loss of fluorescence quenching using an appropriate donor-acceptor pair. Example 2 The thiomethyl linker was introduced by conversion of 5-fluoresceinamine to 5-mercaptofluorescein via diazotization, conversion to the ethylxanthate, and degradation of the xanthate by aqueous acid to the free sulfhydryl. It was coupled to 7-bromoacetamido- cephalosporanic acid by nucleophilic displacement of the bromide by the mercapto group of the fluorescein. 7-Bromoacetamido-cephalosporanic acid had been prepared from 7-aminocephalosporanic acid and bromoacetyl bromide [Bunnell, CA. et al . Industrial manufacture of cephalosporins. In: Beta-Lacta Antibiotics for Clinical Use . Series : Clinical Pharmacology Vol . 4 , edited by Queener, S.F., Webber, J.A. and Queener, S.W. New York: M. Dekker, 1986, p. 255-283] . 13 14 To prepare 7/8- [ (5-diacetylfluorescein) thio] acetamido-3- (acetoxymethyl) - 3-cephem- -carboxylic acid (14) , in a nitrogen atmosphere 130mg (0.29mmol) 5-fluoresceinthiol diacetate were dissolved in 10ml dimethylformamide and added to 120mg (0.31mmol) 7 / S-bromoacetamido-3- (acetoxymethyl) -3-cephem-4-carboxylic acid in 10ml IM potassium phosphate buffer adjusted to pH 8.0. The solution was stirred for 8 hours at room temperature after which the solvents were removed in vacuo. The residue was dissolved in 10ml water and the pH of the solution was carefully adjusted to pH 5 with dilute phosphoric acid. At this point nonpolar byproducts precipitated and were removed by centrifugation. Further acidification to pH 2.7 precipitated the title compound which was collected by centrifugation, washed 3 times with 2 ml diethylether- tetrachloromethane (1:2), and dried in vacuo. λ H NMR (CDC1 3 ) : δ 2.08ppm (s, 3H, acetate), δ 3.36ppm, 3.53ppm (2d, 2H, J=17.3 Hz, C-2) , δ 3.87ppm (s, 2H, side chain methylene), δ 4.88ppm, 5.16ppm (2d, 2H, J-13.6 Hz, C-3'), δ 4.96ppm (d, IH, J=4.9 Hz, C-6), δ 5.81ppm (dd, IH, J«8.2 Hz, J 2 =4.9 Hz, C-7), δ 6.85ppm (m, 4H, xanthene), δ 7.10 (s, 2H, xanthene), δ 7.15ppm (d, IH, J=8.2 Hz, amide), δ 7.69ppm (d, IH, J=8.2 Hz, phthalic) , δ 7.91ppm (d, IH, J=8.2 Hz, phthalic), δ 8.llppm (s, IH, phthalic) . 5-Fluoresceinamine was brominated to generate 5-eosinamine, which was converted into 5-mercaptoeosin in analogous way to the 5-mercaptofluorescein. In a nucleophilic displacement of the cephalosporin acetate by 5-mercaptoeosin diacetate the FRET-cephalosporin was generated as the protected tetraacetyl derivative. To prepare 5-eosinamine, 1 . 1 g (5mmol) 5-fluoresceinamine was suspended in 30ml glacial acetic and 2.06ml (40mmol, 100% excess) bromine was added. With the addition of bromine the fluoresceinamine went into solution. The solution was heated for six hours at 90°C, during which period a white precipitate began to form. An ice-cooled trap attached to the flask kept bromine from escaping into the atmosphere. Excess bromine was then recovered by distillation into a liquid nitrogen cooled collecting flask. One volume of water was added to the acetic acid solution to precipitate any product remaining in solution. The precipitate was collected by filtration and dissolved in IN aqueous sodium hydroxide. 5-Eosinamine was precipitated as the free amine by addition of glacial acetic acid. The eosinamine was dissolved in little chloroform and methanol was added. Concentrating this solution on the rotary evaporator gave 2.56g (3.85mmol, 77%) eosinamine as a fine white powder (the eosinamine- spirolactone) . To prepare 5-eosin-ethylxanthate diacetate, 670mg (lmmol) 5-eosinamine were stirred in 2ml concentrated sulfuric acid and 2ml glacial acetic acid. The suspension was cooled with an ice-salt bath to a few degrees below 0°C, which turned it into a thick paste that was difficult to stir. 200mg (2.9mmol) sodium nitrite in 1ml water were added dropwise over the period of one hour. After another 2 hours at 0°C 20g of ice was slowly added. The flask was put on the high vacuum pump in the cold, to remove excess nitrous gases (caution!!) . Saturated ice-cold aqueous sodium bicarbonate solution was added until the solids dissolved into the dark red solution. 200mg (1.2mmol) Potassium ethylxanthate was added and a pink precipitate formed (5-eosindiazonium xanthate) . A few crystals of nickel (II)chloride catalyzed the conversion of the diazonium salt with evolution of nitrogen. Once nitrogen evolution had ceased the products were precipitated with IN hydrochloric acid. The precipitate was collected by filtration and dried in vacuo. It was treated with acetic anhydride-pyridine (1:1) at 40°C for one hour. After removal of the reagents in vacuo, the residue was chromatographed over silica gel with ethyl acetate-hexane (1:4) as eluent. The desired product eluted first. The yield was llOmg (0.13mmol, 13%) of the title compound as a white powder. For preparation of the disulfide dimer of 5-eosinthiol diacetate (dimer of 15), llOmg (0.13mmol) 5-eosin-ethylxanthate diacetate was stirred in 10ml concentrated (30%) aqueous ammonia and the solution was heated to 70°C. Air was bubbled slowly through the solution to oxidize the thiol to the disulfide in si tu . After 2 hours the solvents were removed on the rotary evaporator at 40°C and the residue was treated with acetic anhydride-pyridine (1:1) . After removal of the reagents in vacuo the residue was chromatographed over silica gel with ethyl acetate-hexane (1:4) as the eluent. Yield was 90mg (60μmol, 91%) of the title product as a white powder. The compound was reduced to the monomer (15) by dissolving it in methanol with addition of sodium acetate and addition of 20 equivalents ercaptoethanol. After 2 hours the methanolic solution was poured into 3 volumes 5% aqueous acetic acid from which the precipitating 5-fluoresceinthiol monomer was collected by centrifugation. The solid was washed with water until no odor of mercaptoethanol remained. Coupling of diacetyl 5-eosinthiol (15) with 7 / 8- [ (5-diacetylfluorescein)thio] acetamido-3- (acetoxymethyl) - 3-cephem-4-carboxylic acid (14) and deacylation with acetylesterase was effected as follows. lOmg (13μmol) 7 / 8- [ (5-Diacetylfluorescein)thio] acetamido-3- (acetoxymethyl) - 3-cephem-4-carboxylic acid and lOmg (13μmol) diacetyl- 5-eosinthiol were dissolved in 200μl dry acetonitrile and the solution was sealed under argon in a glass tube. The tube was kept in an oil bath at 84°C (± 2°C) for 16 hours. Then it was cut open, the solution transferred to a flask and the solvent removed in vacuo. The residue was flash-chromatographed over silica gel with ethyl acetate-methanol-acetic acid (100:1:1) as the eluent. Deprotection of the acetates was achieved by incubating the product with orange peel acetylesterase in 50mM phosphate buffer (pH7) for 24 hours at 37°C. The deacylated product was purified by C lβ reverse phase chromatography. The eluent was a step gradient of 25mM aqueous phosphate buffer (pH7) and methanol. Fluorescein byproducts eluted with 33% and 50% methanol in the eluent, after which the desired product eluted in 66% methanol. The deprotected compound shows little fluorescence •in phosphate buffer as the two hydrophobic dyes stack. The remaining fluorescence is due to fluorescence resonance energy transfer (FRET) . This compound is a good substrate for RTEM β-lactamase and will be referred to as FCE. Cleavage of the compound increases fluorescence at 515nm about 70-fold (Fig. 2) . The fluorescence properties of the compound can be attributed to dye-dimer formation, as FRET increases drastically once methanol is added to the solution. Methanol breaks the hydrophobic interaction that causes the fluorophores to stack. Example 3 (compound 22) The 3' -acetate of 7-aminocephalosporanic acid was displaced by ethylxanthate [Van Heyningen and Brown (1965) , supra] which was hydrolysed to the free sulfhydryl with aqueous acetylhydrazine [Japanese Patent, Kokai 75/18494, CA85 , 97320d] . The sulfhydryl group was reacted with 5-bromoacetamido-rhodol-X in aqueous dimethylformamide. The cephalosporin 7-amine was reacted with bromoacetyl bromide in aqueous dioxane, followed by bromide displacement with 5-fluoresceinthiol to yield a FRET-cephalosporin that is virtually nonfluorescent in 50mM phosphate buffer pH 7. This compound is referred to as FCRX. The first step in preparation of 5-rhodol-X- bromoacetamide was synthesis of 9- (2 ' -carboxy- 4 ' (5 ' ) -nitro-benzoyl) -8-hydroxyjulolidine and separation of the isomers. 10.lg (48mmol,92% purity) 4-Nitrophthalic anhydride were dissolved in 20ml toluene at 70°C. 9.76g (50mmol, 97% purity) 8-Hydroxyjulolidine in 20ml ethyl acetate were added and the solution kept at 70°C for 30min. The reaction mixture was run through a short bed of silica gel followed by ethyl acetate as eluent. The solvents were removed in vacuo and the solid redissolved in a minimum amount of refluxing ethyl acetate. The isomer with the nitro-group meta to the benzoic acid crystallizes over night from solution in orange crystals (3.47g in first fraction) . After additional fractional crystallization the pure isomer was obtained. H NMR (CDC1 3 ) of crystallized isomer: δ 1.91ppm (m, 4H, aliphatic ethylenes) , δ 2.73ppm, 2.46ppm (2m, 4H, anilinic methylenes), δ 3.26ppm (m, 4H, benzylic methylenes) , δ 6.32ppm (s, IH, julolidine) , δ 7.53ppm (d, IH, J=8.4 Hz, phthalic) , δ 8.43ppm (dd, J α =8.4 Hz, J 2 =2.2 Hz, phthalic) , δ 8.90ppm (d, IH, J=2.2 Hz, phthalic) . For preparation of 5-rhodol-X-amine hydrochloride (named by analogy with rhodamine-X) , 1.91g (5.0mmol) 9- (2 ' -Carboxy-4 ' -nitro-benzoyl) -8-hydroxyjulolidine was stirred in 5ml concentrated (96%) sulfuric acid. 700mg (6.4mmol, 1.25equ.) resorcinol was added with cooling over a period of 15 minutes. The suspension was stirred 1.5 hours at room temperature and then poured into 200ml water with vigorous stirring. The purple precipitate was collected by filtration and redissolved in 75ml water with the help of 5.3g (22mmol) sodium sulfide nonahydrate. 2.5g (44.6 mmol) Anhydrous sodium bisulfide was added and the solution refluxed for 24 hours. Then, after cooling to room temperature, the product was precipitated by addition of glacial acetic acid. The solid was collected by filtration and boiled with 100ml half-saturated aqueous hydrochloric acid. The solution was filtered hot through a glass frit to remove sulfur. The solution volume was reduced to 10ml on the rotary evaporator. 1 Volume saturated brine was added and the precipitate collected by filtration. Crystallization from refluxing hydrochloric acid yielded 1.78g (3.85mmol, 77%) dark red crystals of 5-rhodol-X-amine hydrochloride. 1 H NMR (dDMSO) of 5-nitro-rhodol-X: δ 1.90ppm, 2.05ppm (2m, 4H, aliphatic methylenes) , δ 2.72ppm, 3.03ppm (2m, 4H, anilinic methylenes) , δ 3.66ppm (m, 4H, benzylic methylenes) , δ 6.90ppm (s, IH, xanthene) , δ 6.96ppm (dd, IH, J 1 =9.0 Hz, J 2 =2.1 Hz, xanthene) , δ 7.11ppm (d, IH, J=9.0 Hz, xanthene) , δ 7.22ppm (d, IH, J=2.1 Hz, xanthene), δ 7.78ppm (d, IH, J=8.4 Hz, phthalic) , δ 8.70ppm (dd, IH, J---8.4 Hz, J 2 =2.4 Hz, phthalic) , δ 8.91ppm (d, IH, J=2.4 Hz, phthalic) . *H NMR (CD 3 OD) of 5-rhodol-X-amine hydrochloride: δ 2.00ppm, 2.14ppm (2m, 4H, aliphatic methylenes) , δ 2.75ppm, 3. llppm (2m, 4H, anilinic methylenes) , δ 3.67ppm (m, 4H, benzylic methylenes) , δ 6.85ppm (s, IH, xanthene) , δ 6.94ppm (dd, IH, J x =9.0 Hz, J 2 =2.1 Hz, xanthene) , δ 7.13ppm (d, IH, J=9.0 Hz, xanthene), δ 7.16ppm (d, IH, J=2.1 Hz, xanthene), δ 7.55ppm (d, IH, J=8.1 Hz, phthalic) , δ 7.82ppm (dd, IH, J-=8.1 Hz, J 2 =l .9 Hz, phthalic) , δ 8.28ppm (d, IH, J=1.9 Hz, phthalic) . Preparation of 5-rhodol-X-bromoacetamide (18) was effected as follows. 115mg (0.25mmol) 5-Rhodol-X-amine hydrochloride were dissolved with 180mg (2.1mmol) sodium bicarbonate in 2ml water-dioxane (1:1) . The solution was cooled on ice and 175μl (2mmol) bromoacetylbromide were added with stirring over a period of 20 minutes. The solution was then kept at room temperature for 1.5 hours, after which 5 volumes of water were added. The dioxane was removed on the rotary evaporator, and the product was precipitated from the remaining aqueous solution by addition of acetic acid. The precipitate was filtered off and dissolved in a small volume of chloroform-methanol (1:1). Silica gel was added to the solution and the solvents removed in vacuo. The solids were applied to a silica gel column and the product eluted with methanol-ethyl acetate (1:4). This eluent did dissolve some silica gel which remained with the eluted product. H NMR (CD 3 OD,10% dDMSO) : δ 1.98ppm, 2.12ppm (2m, 4H, aliphatic methylenes), δ 2.72ppm, 3.06ppm (2m, 4H, anilinic methylenes), δ 3.56ppm (m, 4H, benzylic methylenes), δ 4.08ppm (s, 2H, bromoacetyl), δ 6.79ppm (dd, IH, J 1= 9.2 Hz, J 2 =2.1 Hz, xanthene), δ 6.83ppm (s, IH, xanthene), δ 6.90ppm (d, IH, J=2.1 Hz, xanthene), δ 7.19ppm (d, IH, J=9.2 Hz, xanthene), δ 7.24ppm (d, IH, J=8.4 Hz, phthalic), δ 8.02ppm (dd, IH, J 1= 8.4 Hz, J 2 «l Hz, phthalic), δ 8.30ppm (d, IH, J-l Hz, phthalic) . For preparation of Iβ- (bromoacetamido) - 3- [ [ [ (5-rhodol-X-amido)methyl] thio]methyl] -3-cephem- 4-carboxylic acid (20), 4.5mg (lOμmol) 5-Rhodol-X- bromoacetamide (18) were dissolved in 0.5ml 250mM phosphate buffer adjusted to pH 7.7 and 0.5ml dimethylformamide. The solution was deoxygenated and lOmg (40μmol) 7 / 3-amino- 3- (thiomethyl) -3-cephem-4-carboxylic acid (8) prepared according to the literature procedure in lOOμl phosphate buffer was added in an argon atmosphere. The solution was kept for 2 hours at 30°C. Then the solvents were removed in vacuo and the residue dissolved in 1 ml water, from which the product was precipitated by addition of acetic acid. The precipitate was collected and the product purified by C 18 reverse-phase chromatography with 0.1% trifluoroacetic acid in 35% methanol/water as eluent. The above product (19) was dissolved in 1ml dioxane- water (1:1) with 20mg sodium bicarbonate. lOμl Bromoacetyl bromide were added to the solution on ice. The solution was kept for another 1.5 hours at room temperature. 20mg sodium bicarbonate and lOμl bromoacetyl bromide were added to the solution with ice cooling. After another 1.5 hours at room temperature the dioxane was removed on the rotary evaporator and the products were precipitated from the aqueous solution with IM phosphoric acid and collected by centrifugation. The solids were suspended in dilute aqueous bicarbonate solution and the undissolved particles removed by centrifugation and discarded. The product was precipitated with IM phosphoric acid and purified by flash chromatography on silica gel with chloroform-methanol-acetic acid-water (55:15:4:2) . This procedure dissolved small amounts of silica gel. Coupling of diacetyl 5-fluoresceinthiol (21) with 7 / 8- (bromoacetamido) -3- [ [ [ (5-rhodol-X- amido) ethyl] thio]methyl] -3-cephem-4-carboxylic acid (20) was effected as follows. 7 / 3- (Bromoacetamido) -3- [[[ (5-rhodol-X- amido) ethyl] thio]methyl] -3-cephem-4-carboxylic acid was reacted with a 50% excess of 5-fluoresceinthiol under argon with dimethylformamide- (250mM aqueous phosphate buffer pH 7.7) (1:1) as the solvent. The product was purified from excess fluoresceinthiol by repeated dissolution in methanol and precipitation in ethyl acetate. Fig. 3 shows the fluorescence emission spectra of this FRET-cephalosporin in 50mM phosphate buffer pH 7 before and after treatment with β-lactamase. The low initial fluorescence is due to the stacking of the fluorophores, forming a ground state complex that is nonfluorescent. When one adds methanol to the solution this stacking can be disrupted and efficient fluorescence resonance energy transfer occurs. Example 4 (compound 25) N- [resorufin-4-carbonyl] -N' -iodoacetyl-piperazine (Boehringer Mannheim) was attached to the cephalosporin as a FRET-acceptor for fluorescein. It is referred to as FCRE. The FRET-cephalosporin FCRE (25) carrying fluorescein as the donor and resorufin as the quencher was made by the same procedure as the one carrying the rhodol-X- acceptor. The N- [resorufin-4-carbonyl] -N' -iodoacetyl- piperazine (Boehringer Mannheim) was coupled to the free 3 '-thiol of the cephalosporin followed by bromoacetylation and addition of the 5-fluoresceinthiol. In departure from the protocol, three equivalents of 5-fluorescein thiol were added, as the first equivalent instantaneously reduced the resorufin and formed unreactive difluorescein-disulfide. Exposure to air reoxidized resorufin to the original dye. 25 /3-Lactamase catalyzed hydrolysis of this compound generates two fluorescent fragments. Resorufin excitation and emission spectra are longer wavelength and narrower than the rhodol spectra, possibly affording better spectral separation between the uncleaved dye versus the products of enzymatic cleavage. But, as in the case of rhodol as the acceptor, in aqueous phosphate buffer the dyes stack and form a dark complex. /3-Lactamase treatment disrupts the stacking and increases donor fluorescence (Fig. 4) . Example 5 (compound 7b) 1 H2NCI-t_C02Bπ. DCC. HBT 2. H2, P C For synthesis of 2,4 dihydroxy-5-chlorobenzaldehyde, 21.7 g (0.15 Mol) 4-chlororesorcinol were dissolved in 150 ml dry diethyl ether and 27 g finely powdered zinc (II) cyanide and 0.5 g potassium chloride were added with stirring. The suspension was cooled on ice. A strong stream of hydrogen chloride gas was blown into the solution with vigorous stirring. After approximately 30 minutes the reactants were dissolved. The addition of hydrogen chloride gas was continued until it stopped being absorbed in the ether solution (approx. 1 hour) . During this time a precipitate formed. The suspension was stirred for one additional hour on ice. Then the solid was let to settle. The ethereal solution was poured from the solid. The solid was treated with 100 g of ice and heated to 100°C in a water bath. Upon cooling the product crystallized in shiny plates from the solution. They were removed by filtration on dried over potassium hydroxide. The yield was 15.9 g (0.092 Mol, 61%) . X H NMR (CDC1 3 ) : δ 6.23ppm (s, IH, phenol) , δ 6.62ppm (s, IH, phenyl) , δ 7.52ppm (s, IH, phenyl) , δ 9.69ppm (s, IH, formyl) , δ 11.25ppm (s, IH, phenol) . To prepare 3-carboxy 6-chloro 7-hydroxy coumarin, 5.76 g (0.033 Mol) 2,4-dihydroxy-5-chlorobenzaldehyde and 7.2 g (0.069 Mol) malonic acid were dissolved in 5 ml warm pyridine . 75 μl Aniline were stirred into the solution and the reaction let to stand at room temperature for 3 days. The yellow solid that formed was broken into smaller pieces and 50 ml ethanol was added. The creamy suspension was filtered through a glass frit and the solid was washed three times with 1 N hydrochloric acid and then with water. Then the solid was stirred with 100 ml ethyl acetate, 150 ml ethanol and 10 ml half concentrated hydrochloric acid. The solvent volume was reduced in vacuo and the precipitate recovered by filtration, washed with diethyl ether and dried over phosphorous pentoxide. 4.97 g (0.021 Mol, 63%) of product was obtained as a white powder. X H NMR (dDMSO) : δ 6.95ppm (s, IH) , δ 8.02ppm (s, IH) , δ 8.67ppm (s, IH) . To prepare 7-butyryloxy-3-carboxy-6-chlorocoumarin, 3.1 g (12.9 mMol) 3-carboxy-6-chloro-7-hydroxycoumarin were dissolved in 100 ml dioxane and treated with 5 ml butyric anhydride, 8 ml pyridine and 20 mg dimethyl aminopyridine at room temperature for two hours. The reaction solution was added with stirring to 300 ml heptane upon which a white precipitate formed. It was recovered by filtration and dissolved in 150 ml ethyl acetate. Undissolved material was removed by filtration and the filtrate extracted twice with 50 ml 1 N hydrochloric acid/brine (1:1) and then brine. The solution was dried over anhydrous sodium sulfate. Evaporation in vacuo yielded 2.63 g (8.47 mMol, 66%) of product. α H NMR (CDC1 3 ) : δ l.Oθppm (t, 3H, J = 7.4 Hz, butyric methyl), δ 1.85ppm (m, 2H, J-~ J 2 = 7.4 Hz, butyric methylene) , δ 2.68ppm (t, 2H, J = 7.4 Hz, butyric methylene) , δ 7.37ppm (s, IH, coumarin) , δ 7.84ppm (s, IH, coumarin) , δ 8.86ppm (s, IH, coumarin) . Preparation of 7-butyryloxy-3- benzyloxycarbonylmethylaminocarbonyl-6-chlorocoumarin is effected as follows. 2.5 g (8.06 mMol) 7-Butyryloxy-3- carboxy-6-chlorocoumarin, 2.36 g hydroxybenztriazole hydrate (16 mMol) and 1.67 g (8.1 mMol) dicyclohexyl carbodiimide were dissolved in 30 ml dioxane. A toluene solution of O- benzylglycine [prepared by extraction of 3.4 g (10 mMol) benzylglycine tosyl salt with ethyl acetate - toluene - saturated aqueous bicarbonate - water (1 : 1 : 1 : 1, 250 ml) , drying of the organic phase with anhydrous sodium sulfate and reduction of the solvent volume to 5 ml] was added dropwise to the coumarin solution. The reaction was kept at room temperature for 20 hours after which the precipitate was removed by filtration and washed extensively with ethylacetate and acetone. The combined solvent fractions were reduced to 50 ml on the rotatory evaporator upon which one volume of toluene was added and the volume further reduced to 30 ml. The precipitating product was recovered by filtration and dissolved in 200 ml chloroform - absolute ethanol (1 : 1) . The solution was reduced to 50 ml on the rotatory evaporator and the product filtered off and dried in vacuo yielding 1.29 g of the title product. Further reduction of the solvent volume yielded a second crop (0.64 g) . Total yield: 1.93g (4.22 mMol, 52%) . ^ NMR (CDC1 3 ) : δ l.Oθppm (t, 3H, J = 7.4 Hz, butyric methyl), δ 1.84ppm (m, 2H, J^- J 2 = 7.4 Hz, butyric methylene), δ 2.66ppm (t, 2H, J = 7.4 Hz, butyric methylene) , δ 4.29ppm (d, 2H, J = 5.5 Hz, glycine methylene) , δ 5.24ppm (s, 2H, benzyl) , δ 7.36ppm (s, IH, coumarin), δ 7.38ppm (s, 5H, phenyl), δ 7.77ppm (s, IH, coumarin) , δ 8.83ppm (s, IH, coumarin), δ 9.15ppm (t, IH, J = 5.5 Hz, amide) . 7-Butyryloxy-3-carboxymethylaminocarbony1-6- chlorocoumarin was prepared as follows. 920 mg (2 mMol) 7- butyryloxy-3-benzyloxycarbonylmethylaminocarbonyl-6- chlorocoumarin were dissolved in 50 ml dioxane. 100 mg palladium on carbon (10%) and 100 μl acetic acid were added to the solution and the suspension stirred vigorously in a hydrogen atmosphere at ambient pressure. After the uptake of hydrogen seized the suspension was filtered. The product containing carbon was extracted five times with 25 ml boiling dioxane. The combined dioxane solutions were let to cool upon which the product precipitated as a white powder. Reduction of the solvent to 20 ml precipitates more product. The remaining dioxane solution is heated to boiling and heptane is added until the solution becomes cloudy. The weights of the dried powders were 245 mg, 389 mg and 58 mg, totaling 692 mg (1.88 mMol, 94%) of white product. X H NMR (dDMSO) : δ 1.02ppm (t, 3H, J = 7.4 Hz, butyric methyl), δ 1.73ppm (m, 2H, J^- J 2 = 7.3 Hz, butyric methylene), δ 2.70ppm (t, 2H, J = 7.2 Hz, butyric methylene), δ 4.07ppm (d, 2H, J = 5.6 Hz, glycine methylene), δ 7.67ppm (s, IH, coumarin), δ 8.35ppm (s, IH, coumarin), δ 8.90ppm (s, IH, coumarin), δ 9.00ppm (t, IH, J = 5.6 Hz, amide) . Coupling of 7-Butyryloxy-3- carboxymethylaminocarbonyl-6-chlorocoumarin with 7-amino-3'- chlorocephalosporanic acid benzhydryl ester was effected as follows. 368 mg (1 mMol) 7-Butyryloxy-3- carboxymethylaminocarbony1-6-chlorocoumarin, 27Omg hydroxybenztriazole hydrate and 415 mg (1 mMol) 7-amino-3'- chloro cephalosporanic acid benzhydryl ester were suspended in 40 ml dioxane - acetonitrile (1 : 1) . 260 mg (1.25 mMol) dicyclohexylcarbodiimide in 5 ml acetonitrile were added and the suspension was stirred vigorously for 36 hours. The precipitate was removed by filtration and the volume of the solution reduced to 20 ml on the rotatory evaporator. 50ml Toluene was added and the volume reduced to 30 ml. With stirring 50 ml heptane was added and the suspension chilled on ice. The precipitate was recovered by filtration. It was redissolved in 10 ml chloroform and the remaining undissolved solids were filtered off. Addition of 2 volumes of heptane precipitated the title product which was collected and dried in vacuo and yielded 468 mg (0.64 mMol, 64%) off-white powder. *H NMR (CDC1 3 ) : δ l.Oβppm (t, 3H, J = 7.4 Hz, butyric methyl), δ 1.84ppm ( , 2H, J-~ J 2 = 7.4 Hz, butyric methylene), δ 2.66ppm (t, 2H, J = 7.4 Hz, butyric methylene), δ 3.54ppm (2d, 2H, J = 18.3 Hz, cephalosporin C-2) , δ 4.24ppm (2d, 2H, J = 5.8 Hz, cephalosporin 3 methylene), δ 4.37ppm (d, 2H, J = 3.8 Hz, glycine methylene), δ 5.02ppm (d, IH, J = 4.9 Hz, cephalosporin C-6), δ 5.89ppm (dd, IH, J x = 9.0 Hz, J 2 = 5.0 Hz, cephalosporin C-7), δ 6.96ppm (s, IH, benzhydryl), δ 7.30- 7.45ppm (m, 12H, phenyl, coumarin, amide), δ 7.79ppm (s, IH, coumarin), δ 8.84ppm (s, IH, coumarin), δ 9.28ppm (t, IH, J = 3.7 Hz, amide) . Coupling of the above product with 5- fluoresceinthiol was effected as follows. 90 mg (0.2 mMol) 5- mercaptofluorescein diacetate disulfide dimer were dissolved in 10ml chloroform and treated with 25 μl tributyl phosphine and 25 μl water in an argon atmosphere. The solution was kept for 2 hours at ambient temperature and was then added to a solution of 20 mg sodium bicarbonate, 25mg sodium iodide and 110 mg (0.15 mMol) of the above compound in 10 ml dimethylformamide. After 4 hours the solvents were removed in vacuo and the residue triturated with diethylether. The solid was dissolved in ethyl acetate - acetonitrile (1:1). After removal of the solvents the residue was triturated once more with diethylether yielding 157 mg (0.13mMol, 88%) of a cream colored powder product. A sample of the above compound was treated with a large access of trifluoroacetic acid - anisole (1:1) at room temperature for 20 minutes. The reagents are removed in vacuo and the residue triturated with ether. High performance liquid chromatography of the solid in 45% aqueous acetonitrile containing 0.5% acetic acid gives a product in which the butyrate and the diphenylmethyl esters have been cleaved. It was purified by high performance liquid chromatography on a reverse phase C 18 -column using 45% aqueous acetonitrile containing 5% acetic acid as the eluent. N«HCθ 3 , H^-CH 3 OH 7b Deprotection of the fluorescein acetates in compound 27 was accomplished with sodium bicarbonate in methanol (room temperature, 30minutes) to provide the fluorescent enzyme substrate CCF2. It was purified by high performance liquid chromatography on a reverse phase C 18 - column using 35% aqueous acetonitrile containing 0.5% acetic acid as the eluent. Stirring of compound 27 with excess acetoxymethyl bromide in dry lutidine produced the membrane permeable derivative of the substrate (CCF2/ac 2 AM 2 ) . It was purified by high performance liquid chromatography on a reverse phase C 18 - column using 65% aqueous acetonitrile containing 0.5% acetic acid as the eluent. CCF2/ac 2 AM 2 is readily converted to CCF2 in the cells' cytoplasm. Unlike in Examples 1-4, the donor and acceptor dyes in substrate CCF2 do not stack. The substrate is fully fluorescent in phosphate buffer and there is no formation of the "dark complex" (i.e., addition of methanol does not change the fluorescence spectrum of CCF2, except for the effect of dilution) . This is due to the much smaller and more polar nature of the 7-hydroxycoumarin compared to that of the xanthene dyes (eosin, rhodamine, rhodol and resorufin) in Examples 1-4. Fig. 5 illustrates the emission spectrum of compound CCF2 in 50 mmolar phosphate buffer pH 7.0 before and after β- lactamase cleavage of the /.-lactam ring. In the intact substrate, efficient energy transfer occurs from the 7- hydroxycoumarin moiety to the fluorescein moiety. Excitation of the substrate at 405 nm results in fluorescence emission at 515 nm (green) from the acceptor dye fluorescein. The energy transfer is disrupted when / 3-lactamase cleaves the / 3-lactam ring, thereby severing the link between the two dyes. Excitation of the products at 405 nm now results entirely in donor fluorescence emission at 448 nm (blue) . The fluorescence emission from the donor moiety increases 25 fold upon / 3-lactam cleavage. The fluorescence at 515 nm is reduced by 3.5 fold, all of the remaining fluorescence originating from the 7-hydroxycoumarin as its emission spectrum extends into the green. Twenty-five-fold quenching of the donor in the substrate is equivalent to an efficiency of fluorescence energy transfer of 96%. This large fluorescence change upon / S-lactam cleavage can readily be used to detect β-lactamase in the cytoplasm of living mammalian cells, as is reported in Examples 6 and 7. The 7-hydroxycoumarin moiety in the cephalosporin was determined to have a fluorescence quantum efficiency in the absence of the acceptor of 98-100%. This value was determined by comparing the integral of the corrected fluorescence emission spectrum of the dye with that of a solution of 9-aminoacridine hydrochloride in water matched for absorbance at the excitation wavelength. It follows that 7- hydroxycoumarin is an ideal donor dye, as virtually every photon absorbed by the dye undergoes fluorescence energy transfer to the acceptor. Example 6 Cells of the T-cell lymphoma line Jurkat were suspended in an isotonic saline solution (Hank's balanced salt solution) containing approximately 10 12 /3-lactamase enzyme molecules per milliliter (approximately 1.7 nM; Penicillinase 205 TEM R * , from Sigma) and 1 mg/ l rhodamine conjugated to dextran (40 kd) as a marker of loading. The suspension was passed through a syringe needle (30 gauge) four times. This causes transient, survivable disruptions of the cells' plasma membrane and allows entry of labeled dextran and /S-lactamase. Cells that had been successfully permeabilized contained β- lactamase and were red fluorescent when illuminated at the rhodamine excitation wavelength on a fluorescent microscope. The cells were incubated with 5μM fluorogenic β-lactamase substrate, CCF2/ac 2 AM 2 , at room temperature for 30 minutes. Illumination with violet light (405 nm) revealed blue fluorescent and green fluorescent cells. All cells that had taken up the marker rhodamine-dextran appeared fluorescent blue, while cells devoid the enzyme appeared fluorescent green. Example 7 Cells from cell lines of various mammalian origin were transiently transfected with a plasmid containing the RTEM jβ-lactamase gene under the control of a mammalian promotor. The gene encodes cytosolic β-lactamase lacking any signal sequence and is listed as SEQ. ID. 1. 10 to 48 hours after transfection cells were exposed to 5 μmol CCF2/ac 2 AM 2 for 1 to 6 hours. In all cases fluorescent blue cells were detected on examination with a fluorescence microscope. Not a single blue fluorescent cell was ever detected in nontransfected control cells. To quantitate the fluorescence measurements the cells were first viewed through coumarin (450 DF 65) and then fluorescein (515 EFLP) emission filters and pictures were recorded with a charge couple device camera. The average pixel intensities of CCF2 loaded transfected cells (blue) and controls (green) at coumarin and fluorescein wavelength in COS-7 (Table 2) and CHO (Table 3) cells are summarized; values for 4 representative cells for each population are given. Thus, the substrate CCF2 revealed gene expression in single living mammalian cells. Table 2 COS-7 (origin: SV40 transformed african green monkey kidney cells) Table of pixel coumarin fluorescein intensities emission filter emission filter Blue cell #1 27 20 #2 34 23 #3 31 31 #4 22 33 Green cel_#l 4 43 #2 4 42 #3 5 20 #4 3 24 Table 3 CHO (origin: Chinese hamster ovary cells) coumarin fluorescein emission filter emission filter 98 112 70 113 76 92 56 67 9 180 9 102 7 101 9 83 Example 8 For preparation of 7-acetyloxy-3- (N-carboxymethyl-N- methylaminocarbonyl)coumarin, 400 mg (1.6 mMol) 3-carboxy-7- acetylcoumarin were refluxed with 4 ml thionyl chloride for 20 minutes. Excess thionyl chloride was removed by distillation and the residue (7-acetyioxy-3-chlorocarbonylcoumarin) stored in vacuo over potassium hydroxide pellets overnight. In a separate vessel 142.5 mg (1.6 mMol) sarcosine was dissolved in 1.05 ml (5.4 mMol) N-methyl trimethylsilyl trifluoroacetamide (MSTFA) and kept at room temperature for 16 hours. 2 ml dry acetonitrile and 187 μl (1.7 mMol) N-methylmorpholine were added and the solution was poured onto the solid 7-acetyloxy- 3-chlorocarbonylcoumarin on ice. After stirring for 20 minutes on ice the solution was let to warm to room temperature. After 4 hours the solvents were removed in vacuo. The residue was dissolved in methanol to deprotect the acid after which the solvent was removed in vacuo. The solid was dissolved in 30 ml ethylacetate-acetonitrile (2:1) and the solution extracted twice with an equal volume of 1 N hydrochloric acid and the with brine. The organic phase was dried over anhydrous sodium sulfate. The solvent was removed in vacuo and the solid crystallized from boiling ethylacetate with addition of hexane. The yield was 316mg (1.0 mMol, 63%) of a white crystalline solid. Coupling of 7-acetyloxy-3- (N-carboxymethyl-N- methylaminocarbonyl) coumarin with 7-amino-3'- chlorocephalosporanic acid benzhydryl ester was effected as follows. 62 mg (0.2 mMol) 7-acetyloxy-3- (N-carboxymethyl-N- methylaminocarbonyl) coumarin was stirred with 1 ml dry methylene chloride to which 27 mg (0.2 mMol) hydroxybenztriazole and 41 mg dicyclohexyl carbodiimide had been added. A solution of 82.6 mg (0.2 mMol) 7-amino 3'- chloro cephalosporanic acid benzhydryl ester in 1 ml methylene chloride was added dropwise over a period of 5 minutes . The reaction was stirred for 20 hours at room temperature after which the precipitate was removed by filtration. The filtrate was evaporated in vacuo and the product extracted into methylene chloride. The solvent was removed once more and the residue dissolved in 1 ml ethyl acetate. Addition of three volumes of hexane precipitated the product which was recovered by centrifugation. The yield was 49.9 mg (70 μMol, 35%) of the product as a white powder. Conversion of the cephalosporin 3 '-chloro substituent in the above product to the 3' -iodo substituent was carried out as follows. 49.9 mg (70 μMol) of the above product was stirred with 52.5 mg sodium iodide (5 equivalents) in 1.2 ml dry methyl ethyl ketone at room temperature for 2 hours. The solvent was removed in vacuo and the residue dissolved in 2 ml ethyl acetate - methylene chloride (1:1) and extracted with cold 2% aqueous sodium thiosulfate solution, followed by two extractions with brine. The organic layer was dried over anhydrous sodium sulfate. The slightly orange powder (32 mg, 40 μMol, 57%) was used without further purification in the next reaction. Coupling of above product with 5-mercaptofluorescein diacetate (product CCFlac 3 diphenylmethyl ester) was effected by dissolving 32 mg (40 μMol) of the iodo derivative in 0.4 ml dimethylformamide and 3.4 mg sodium bicarbonate added. 22 mg (50 μMol) 5-mercaptofluorescein diacetate were dissolved in 0.3 ml deoxygenated dimethyl formamide and added to the iodo compound in an argon atmosphere. After 2 hours the solvent was removed in vacuo. The residue was suspended in methylene chloride - ethyl acetate (1:1) . The organic solution was washed with water and dried over anhydrous sodium sulfate. The solvent was removed and the residue was triturated with ethyl ether hexane (1:1). Flash chromatography on 60 mesh silica gel with ethyl acetate - toluene (2:1) yielded 4.2 mg (4 μMol, 10%) of colorless product. Cleavage of the diphenylmethyl ester to give CCFlac 3 was effected as follows. 4 mg (4 μMol) of CCFlac 3 diphenylmethyl ester were treated with 200 μl trifluoroacetic acid - anisole - methylene chloride (10:1:10) on ice for 15 minutes. The reagents were removed in vacuo and the residue was dissolved in 0.5 ml ethyl acetate and the solvent evaporated in vacuo. The solid was triturated with ether and then dissolved in 0.5 ml methanol. Addition of the methanolic solution to 2 ml water precipitated the product. The product was recovered by centrifugation and dried in vacuo. The yield was 2mg (2 μMol, 50%) white solid. The compound was further purified by high performance liquid chromatography on a reverse phase C 18 -column using 55% aqueous acetonitrile containing 0.5% acetic acid as the eluent. The fluorescence emission spectrum of CCFl before and after /3-lactamase cleavage (Fig. 6) was obtained from a sample of CCFlac 3 that had been converted to CCFl by treatment with orange peel acetyl esterase in 50 mmolar aqueous phosphate buffer pH 7. Substrate CCFl has similar fluorescence properties to substrate CCF2 in Example 5. In the intact substrate, efficient energy transfer occurs from the 7-hydroxycoumarin moiety to the fluorescein moiety. Excitation of the substrate at 390 nm results in fluorescence emission at 515 nm (green) from the acceptor dye fluorescein. The energy transfer is disrupted when .-lactamase cleaves the jβ-lactam ring, thereby severing the link between the two dyes. Excitation of the products at 390 nm now results entirely in donor fluorescence emission at 460 nm (blue) . The fluorescence emission from the donor moiety increases 25-fold upon / 3-lactam cleavage. The fluorescence at 515 nm is reduced by 3-fold, all of the remaining fluorescence originating from the 7-hydroxycoumarin as its emission spectrum extends into the green. Twenty-five¬ fold quenching of the donor in the substrate is equivalent to an efficiency of fluorescence energy transfer of 96%. This large fluorescence change upon /3-lactam cleavage can readily be used to detect β-lactamase in the cytoplasm of living mammalian cells, as is reported in Example 9. Example 9 Cells of the T-cell lymphoma line Jurkat wer≤ suspended in an isotonic saline solution (Hank's balanced salt solution) containing approximately 10 12 /3-lactamase enzyme molecules per milliliter (approximately 1.7 nM; Penicillinase 205 TEM R*, from Sigma) and 1 mg/ml rhodamine conjugated to dextran (40 kd) as a marker of loading. The suspension was passed through a syringe needle (30 gauge) four times. This causes transient, survivable disruptions of the cells' plasma membrane and allows entry of labeled dextran and /S-lactamase. Cells which had been successfully permeabilized contained β- lactamase and were red fluorescent when illuminated at the rhodamine excitation wavelength on a fluorescent microscope. The cells were incubated with 30μM fluorogenic /3-lactamase substrate CCFlac 3 at room temperature for 30 minutes. Illumination with ultraviolet light (360 nm) revealed blue fluorescent and green fluorescent cells. All cells that had taken up the marker rhodamine-dextran appeared fluorescent blue, while cells devoid the enzyme appeared fluorescent green. Example 10: The preferred membrane-permeable ester of CCF2 was prepared as follows: CCF2/btAMac 2 Coupling of 5-fluoresceinthiol diacetate (5) and 7- amino-3' -chlorocephalosporanic acid benzhydryl ester was effected as follows. 450 mg (lmmol) 5-mercaptofluorescein diacetate disulfide dimer were dissolved in 30ml chloroform and treated with 50 μl water and 125 μl tributylphosphine in a nitrogen atmosphere which generated the free 5- fluoresceinthiol. 450 mg (lmmol) 7-Amino-3'- chlorocephalosporanic acid benzhydryl ester hydrochloride salt were dissolved in 10 ml acetonitrile with the help of 220 μl (2 mmol) N-methyl morpholine and the two solutions combined after 30 minutes. One hour later the solvent volume was reduced to 5 ml and 50 ml carbon tetrachloride were added. The solvent volume was reduced to 15 ml and hexane added with stirring. The initial orange precipitate consisting mainly of N-methyl morpholine hydrochloride was removed by filtration. Upon further addition of two volumes of hexane 630 mg (0.76 mmol, 76%) white product was precipitated and collected. The above product was coupled with 7-butyryloxy-3- carboxymethyl aminocarbonyl-6-chlorocoumarin. 325 mg (0.88 mmol) 7-Butyryloxy-3-carboxymethyl aminocarbonyl-6- chlorocoumarin was dissolved in 15 ml hot dry dioxane. With rapid cooling 110 μl (1 mmol) N-methyl morpholine in 1 ml dioxane and 115 μl (0.9 mmol) isobutyl chloroformate in 8 ml methylene chloride were added. The reaction was kept at 0°C for 30 minutes after which 661 mg (0.8 mmol) of the above fluorescein-cephalosporin adduct in 7 ml dry methylene chloride were added. The solution was let to warm to room temperature and after 3 hours the solvents were removed in vaccuo. The residue was dissolved in 30 ml methylene chloride and twice extracted with one volume 10% aqueous acetic acid and once with water. The organic phase was dried over anhydrous sodium sulfate. Addition of 150 ml dry ethanol, reduction of the solvent volume to 50 ml and cooling to -20°C resulted in precipitation of the product (crude, 850mg) . Purification was achieved by chromatography over silica gel with 25% ethyl acetate in toluene as the eluent. 250 mg (0.21 mmol, 26%) of white powderous product was collected. Cleavage of the cephalosporin benzhydryl ester was accomplished by treatment with trifluoroacetic acid. 145 mg (0.12 mmol) of the above product was treated with trifluoroacetic acid / methylene chloride / anisole (10 / 10 / 1) at 0°C for 20 minutes. The reagents were removed in vacuo and the residue triturated with diisopropyl ether. The solid was dissolved in 1 ml dimethyl sulfoxide and the product precipitated by addition to 25 ml water. It was further purified on reverse phase C 18 resin with a step gradient of 40 to 60% aqueous acetonitrile containing 0.5% acetic acid as the eluent yielding 74 mg (73 μmol, 60%) white powder. Protection of cephalosporin acid as membrane permeable acetoxymethyl ester was achieved as follows. 15 mg (15 μmol) of the above product was dissolved in 250 μl methylene chloride. 25 μl Bromomethyl acetate and 50 μl lutidine were added to the solution. The reaction was kept at ambient temperature for 7 hours after which the reagents were removed in vacuo. The residue was purified by flash chromatography on silica gel with ethyl acetate as the eluent. 15 mg (14 μmol, 92%) white product was obtained. This compound, named CCF2/btAMac 2ι was used for intracellular detection of /3-lactamase activity. Example 11: Measurement of activation of an intracellular receptor: Activation of the intracellular glucocorticoid receptor was measured by its ability to upregulate the transcriptional activity of the glucocorticoid responsive element in the mouse mammary tumor virus promotor. This response to steroids was detected as increased intracellular β-lactamase activity on the substrate CCF2 causing an appropriate change in fluorescent signal. The gene for plasmid encoded RTEM / S-lactamase of Escherichia coli without a signal sequence (Sequence 1 of Fig. 7) was put under transcriptional control of the mouse mammary tumor virus promotor and introduced into a mammalian expression vector. This vector also carried the chloramphenicol resistance marker for amplification of the plasmid in bacteria and the neomycin resistance marker for mammalian selection. It was introduced into baby hamster kidney (BHK) cells in culture using the calcium phosphate precipitation technique. Cells were then subjected to selection for stable integration of the plasmid into the cells' genome using the antibiotic G418. One of twenty clones was selected for its marked increase in β-lactamase expression following exposure to the steroid analog dexamethasone. The following describes the measurement of the increase in β-lactamase gene expression in this clone after addition of the agonist dexamethasone. Cells of the stable BHK cell clone G941 expressing / S-lactamase under control of the glucocorticoid-inducible promotor were kept in the presence or absence of the agonists in the incubator at 37 °C. Flasks with cells were removed from the incubator at different intervals after agonist addition and the cells transferred into Hank's balanced salt solution containing 10 μmolar CCF2/btAMac 2 . This compound becomes converted to the β- lactamase accessible fluorescent substrate CCF2 by endogenous cytoplasmic esterases. Ten minutes later the cell supernatant containing CCF2/btAMac 2 was removed. 30 Minutes later the cells were imaged with a cooled CCD camera mounted on an epi- fluorescence microscope. Fluorescence measurements were taken with violet excitation light (filter 400DF15) and with blue (filter 450DF65) and green (filter 535DF45) emission filters. A ratio of blue versus green emission intensities was determined. The ratio is a measure of how much substrate has been converted to product. Using a 40x objective, 4 fields with approximately 60 cells each were imaged at each time point. The results show a significant increase in the ratio of fluorescent intensities reflective of increasing β- lactamase expression and production. Example 12 : Measurement of cell surface receptor activation and intracellular signaling via second-messenger responsive elements: Activation of cell surface receptors leads to a change in intracellular messenger concentrations which in turn modulates intracellular transcription factor activity. In lymphocytes, an increase the intracellular concentration of the messenger ion calcium leads to the activation of the nuclear factor of activated T-lymphocytes (NFAT) . This event increases transcription at promoters containing the NFAT- recognition site. An increase in calcium levels alone is sufficient to markedly increase transcription of a reporter gene such as β-lactamase regulated when it is put under transcriptional control of a promotor containing a trimer of NFAT sites. The murine T-lymphocyte cell line B3Z was transiently cotransfected with two plasmids. One plasmid contained the /3-adrenergic receptor, which localizes at the cells' surface, under the transcriptional control of the strong and constitutively active cytomegalovirus (CMV) promoter. The other plasmid contained the bacterial RTEM β- lactamase gene from Escherichia coli modified for improved mammalian expression (sequence ID # 3 , with optimum mammalian Kozak sequence, /3-globin leader sequence, pre-sequence removed) under the transcriptional control of a promotor containing a trimer of NFAT sites . The plasmids were introduced into cells using electroporation. 5xl0 6 cells in 0.5 ml electroporation buffer were electroporated in the presence of 10 μg each of both plasmids using the Biorad Gene Pulser (250V, 960 μF, 16 μsec) . Twenty-four hours after transfection, cells were either incubated in the presence or absence of the / S-adrenergic agonist isoproterenol (10 μmolar) for 5 hours. The supernatant was removed and replaced with Hank's balanced salt solution containing 10 μmolar CCF2/btAMac 2 . After 20 minutes at room temperature cells were washed with fresh buffer and viewed with the fluorescence microscope. 4% of isoproterenol treated cells appeared fluorescent blue (excitation filter 400DF15, emission filter 435 nm longpass) while no blue fluorescent cells were detectable in the control population (absence of agonist) . Maximal stimulation with 2 μM ionomycin and 50 ng/ml phorbol ester for 5 hours resulted in 20% blue fluorescent cells in the population. Example 13: /3-Lactamases from different microorganisms were modified for use as reporter enzymes in eukaryotic cells, preferably mammalian. The bacterial gene for these enzymes includes a N-terminal pre-sequence (first 23 amino acids of Sequence 2 of Fig. 7.) that targets the enzyme to the extracellular space. Following translocation a pre-sequence peptidase cleaves the 23 amino acid pre-sequence releasing the mature / 3-lactamase enzyme. RTEM β-lactamase from Escherichia coli including its bacterial pre-sequence (Sequence 2 of Fig. 7) was put into a mammalian expression vector under the control of the mouse mammary tumor virus promotor. This construct was introduced into baby hamster kidney cells using the standard calcium phosphate precipitation technique. The /.-lactamase activity was found in the cell culture medium; no activity could be detected in the cell pellet. The amount of /3-lactamase activity in the medium was steroid dependent. Cells that had been in the presence of 1 μM dexamethasone for 36 hours prior to the measurement produced threefold more enzyme than control. This makes the /.-lactamase with its bacterial pre-sequence (Sequences 2 of Fig. 7) useful for an extracellular assay of mammalian reporter gene activity. A preferred use of the / 3-lactamase reporter is where the enzyme is produced and retained in the cell cytoplasm. Therefore the bacterial signal sequence was removed and replaced by ATG (methionine) as the new translational start site in three modified RTEM / 3-lactamase genes (Sequences 1, 3, and 4 of Fig. 7) . In order to increase expression of the β- lactamases in mammalian cells, the RTEM / 8-lactamases of Sequence 3 and 4 of Fig. 7 were constructed with altered ribosome binding sites optimized for mammalian expression [Kozak, M., J. Cell Biol . 108: 229-241 (1989)] . For increased compatibility with the mammalian translation machinery, β- lactamase of sequence ID #3 was inserted at the end of an untranslated mammalian /.-globin leader sequence. All of these novel DNA sequences encoding novel / 3-lactamases were inserted into mammalian expression vectors with the cytomegalovirus promotor controlling their transcription. Mammalian cells in tissue culture (Hela, COS-7, CHO, BHK) were transfected transiently with the plasmids using the standard lipofectin technique. Two to five days after transfection, the cells were incubated with the membrane-permeant derivative, CCF2/btAMac 2 , of the fluorescent substrate CCF2 to assay functional expression of the enzyme. 5-20% of cells transfected with plasmids containing cDNA Sequences 2, 3 and 4 of Fig. 7 showed a conversion of green to blue fluorescence indicating cleavage of the intracellularly trapped substrate by expressed β-lactamase. By contrast, in untransfected or mock transfected controls, all cells showed the green fluorescence of uncleaved CCF2,- no blue-fluorescing cells were observed, confirming the absence of any endogenous / S-lactamase activity. The gene for Bacillus licheniformis / 3-lactamase was isolated from total Bacillus licheniformis DNA by use of the polymerase chain reaction. The oligonucleotide primers removed the / 3-lactamase secretion sequence and generated the DNA sequence ID # 5. This gene was inserted in a pCDNA3 mammalian expression vector under the transcriptional control of the constitutively active cytomegalovirus promoter. HeLa cells were transfected with 10 μg of plasmid per 25 cm 2 culture dish using lipofectin. 5 days after transfection, cells were tested for functional expression of / 8-lactamase by incubating them in the presence of 100 μmolar CCF2/btAMac 2 and visual inspection with the epifluorescence microscope. 30-40% of cells showed blue fluorescence, whereas only green- fluorescing cells, no blue-fluorescing cells were detectable in untransfected controls. In transient transfections, it is typical for <50% of the cells to become transfected. Example 14 A plasmid was constructed with (8-lactamase of sequence ID 3 (figure 7) under control of yeast elongation factor EF-lalpha enhancer and promoter. This plasmid was coinjected together with the potassium salt of substrate CCF2 (compound 7b) into zebrafish embryos at the single cell stage. As control, embryos were injected with the potassium salt of substrate CCF2 alone. After three hours, the embryos were viewed with an epifluorescence microscope using violet excitation light (filter 400DF15) and a 435 nm longpass emission filter. Embryos that had received plasmid DNA fluoresced blue while controls fluoresced green. Example 15 The / 3-lactamase gene of sequence ID 3 was cloned into a Drosophila transformation vector under the control of the glass promotor and injected into wild-type Drosophila embryos. As control, the / 3-lactamase gene was inserted in the wrong orientation. Drosophila embryos were germline- transformed using P element-mediated transformation. The transformations and all subsequent fly manipulations were performed using standard techniques [Karess, R.E. and Rubin, G.M., Cell 3J3, 135, (1984)]. Omatidia of late stage transformed pupe were transsected and dissociated to single cells. The cells were incubated in buffer with 40 μmolar CCF2/btAMac 2 (compound 34) for 20 minutes, washed and viewed with an epifluorescence microscope (excitation filter 400DF15, emission filter 435 nm long pass) . Omatidia cells from flyes transformed with the / 8-lactamase gene in the proper orientation fluoresced blue, while omatidia cells containing the gene in the wrong orientation fluoresced green. Example 16 In certain embodiments the compound of this invention can be any of the following compounds. wherein R γ is selected from the group consisting of H, Cl, and Br; R x is selected from the group consisting of H, and methyl; wherein R* and R zl are independently selected from the group consisting of -C(0)alk, -CH 2 0C(0) alk, -CH 2 SC(O) alk, - CH 2 0C(0) Oalk, lower acyloxy-alpha-benzyl, and deltabutyrolactonyl; wherein alk is lower alkyl of 1 to 4 carbon atoms, and membrane-permeant fluorogenic derivatives thereof, R " is 1- (acyloxy) alkyl. Another example of the compound is : wherein R z and R zl are independently selected from the group consisting of -C(0)alk, -CH 2 0C(O) alk, -CH-SC (O) alk, - CH 2 0C(0)alk, lower acyloxy-alpha-benzyl, and deltabutyrolactonyl; wherein alk is lower alkyl of 1 to 4 carbon atoms. Another example of the compound is A final example of the compound is All publications and patent documents cited in this application are incorporated by reference in their entirety for all purposes to the same extent as if each individual publication or patent document were so individually denoted. The present invention provides novel substrates for beta-lactamase, beta-lactamases and methods for their use. While specific examples have been provided, the above description is illustrative and not restrictive. Many variations of the invention will become apparent to those skilled in the art upon review of this specification. The scope of the invention should, therefore, be determined not with reference to the above description, but instead should be determined with reference to the appended claims along with their full scope of equivalents. All publications and patent documents cited in this application are incorporated by reference in their entirety for all purposes to the same extent as if each individual publication or patent document were so individually denoted.

Claims: WHAT IS CLAIMED IS: 1. A compound of the formula (I) : wherein : one of X and Y is a fluorescent donor moiety or a membrane-permeant derivative thereof, and the other is a quencher moiety, an acceptor fluorophore moiety or a membrane- permeant derivative thereof; R' is selected from the group consisting of H, lower alkyl, (CH 2 ) n OH, (CH 2 ) n C00R", and =N0J, in which n is 0 or an integer from 1 to 5 and J is H, Me, CH 2 C00H, CHMeCOOH, and CMe 2 COOH; R" is selected from the group consisting of H, physiologically acceptable metal and ammonium cations, - CHR 2 OCO(CH 2 ) n CH 3 , -CHR 2 0C0C(CH 3 ) 3 , acylthiomethyl, acyloxy-alpha- benzyl, delta-butyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, betamorpholinoethyl, dialkylaminoethyl, dialkylaminocarbonyloxymethyl, in which R 2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, 0, SO, S0 2 and CH 2 ; Z' is a linker for X; and Z" is a linker for Y. 2. The compound of claim 1, wherein Z' is selected from the group consisting of a direct bond, -(CH 2 ) n C0NR 2 (CH 2 ) m -, - (CH 2 ) n NR 2 CO (CH 2 ) m -, - (CH 2 ) n NR 3 C0NR 2 (CH 2 ) m - , - (CH 2 ) n NR 3 CSNR (CH 2 ) m , - (CH 2 ) n C0NR 3 (CH 2 ) p CONR 2 (CH 2 ) m - , -(CH 2 ) n -, - (CH 2 ) n NR 3 CO(CH 2 ) p S(CH 2 ) m -, - (CH 2 ) n S(CH 2 ) m - , - (CH 2 ) n 0(CH 2 )„- , -(CH 2 ) n NR 2 (CH 2 ) m -, -(CH 2 ) n S0 2 NR 2 (CH 2 )_-, - (CH 2 ) n C0 2 (CH 2 ) m -, wherein R 2 and n are as previously defined; R 3 is selected from the group consisting of hydrogen and lower alkyl; and each of m and p is independently selected from the group consisting of integers from 0 to 4. 3. The compound of claim 1 wherein Z" is selected from the group consisting of a direct bond to a heteroatom selected from the group consisting of 0, N and S in the chromophore, -0(CH 2 ) n -, -S(CH 2 ) n -, -NR 2 (CH 2 ) n -, -N*R 2 2 (CH 2 ) n -, -0C0NR 2 (CH 2 ) n -, -0 2 C(CH 2 ) n -, -SCSNR 2 (CH 2 ) n -, SCS0(CH 2 ) n -, -S(CH 2 ) n CONR 2 (CH 2 ) m , -S(CH 2 ) n NR 2 C0(CH 2 ) m , and wherein R 2 and n are as previously defined; and m is an integer from 0 to 4. 4. The compound of claim 1 wherein R' is selected from the group consisting of H and methyl. 5. The compound of claim 4 wherein R ' is H. 6. The compound of claim 1 wherein R " is selected from the group consisting of H and acetoxymethyl. 7. The compound of claim 1 wherein R 2 is H. 8. The compound of claim 1 wherein A is -S- . 9. The compound of claim 1 wherein one of X and Y is a membrane-permeant derivative of the fluorescent donor moiety, or a membrane-permeant derivative of the quencher moiety or acceptor fluorophore moiety, and wherein at least one of X and Y contains at least one acylated aromatic hydroxyl, acylated amine, or alkylated aromatic hydroxyl wherein the acyl group contains 1 to 5 carbon atoms and wherein the alkyl group is selected from the group consisting of -CH 2 OC(0)alk, -CH 2 SC(O)alk, -CH 2 OC(0)Oalk, lower acyloxy-alpha- benzyl, and deltabutyrolactonyl; wherein alk is lower alkyl of 1 to 4 carbon atoms. 10. The compound of claim 9 wherein at least one of X and Y contains at least one acylated aromatic hydroxyl, wherein the acyl group is either acetyl, n-propionyl, or n- butyryl. 11. The compound of claim 1 wherein said donor is a coumarin of formulae II-VII and said quencher or acceptor is selected from the group consisting of fluoresceins, rhodols, and rhodamines of formulae VIII-XII, XLVII, and XLVII. 12. The compound of claim 11 wherein said donor is selected from the group consisting of coumarins of formulae II-VII and fluoresceins of formula VIII. 13. The compound of claim 1 wherein said quencher or acceptor is selected from the group consisting of fluoresceins, rhodols, and rhodamines of formulae VIII-XII. 14. The compound of claim 13 wherein said donor is a fluorescein of formula VIII and said quencher or acceptor is selected from the group consisting of rhodols and rhodamines of formulae VIII-XII. 15. The compound of claim 12 wherein said coumarin is selected from the group consisting of 7-hydroxycoumarin and 7-hydroxy-6-chlorocoumarin and said fluorescein is selected from the group consisting of fluorescein and dichlorofluorescein. 16. The compound of claim 13 wherein said donor is a fluorescein and said quencher or acceptor is selected from the group consisting of an eosin or a tetrachlorofluorescein of formula VIII in which R a , R b , R°, and R d are Br or Cl. 17. The compound of claim 13 wherein said quencher or acceptor is a rhodol of formulae VIII, IX, or XI. 18. The compound of claim 13 wherein said quencher or acceptor is a rhodamine of formulae VIII, X, or XII. 19. The compound of claim 9 that is membrane- permeant derivatives wherein at least one of X and Y contains an acetoxymethyl group on an aromatic hydroxyl group. 20. The compound of claim 2 wherein Z ' is -(CH 2 ) n CONR 2 (CH 2 ) m -. 21. The compound of claim 20 wherein n and m are 0. 22. The compound of claim 20 wherein R 2 is H. 23. The compound of claim 3 wherein Z" is -S(CH 2 ) n -. 24. The compound of claim 23 wherein n is 0. 25. The compound of claim 12 of the formula R γ is selected from the group consisting of H, Cl, and Br; R x is selected from the group consisting of H, and methyl; wherein R z and R zl are independently selected from the group consisting of -C(0)alk, -CH 2 OC(0) alk, -CH 2 SC (O) alk, - CH 2 OC (O) Oalk, lower acyloxy-alpha-benzyl, and deltabutyrolactonyl; wherein alk is lower alkyl of 1 to 4 carbon atoms, and membrane-permeant fluorogenic derivatives thereof, R " is 1- (acyloxy) alkyl 26. The compound of claim 25 wherein R z and R 21 are selected from the group consisting of acetyl, butyryl, and acetoxymethyl. 27. The compound of claim 12 of the formula: wherein R γ is selected from the group consisting of H, Cl, and Br; and R x is selected from the group consisting of H, and methyl. 28. The compound of claim 14 of the formula (11) RCF 11 and membrane-permeant fluorogenic derivatives thereof 29. The compound of claim 16 of the formula (16! wherein R z and R zl are independently selected from the group consisting of -C(0)alk, -CH 2 OC(O) alk, -CH 2 SC(O)alk, - CH 2 OC(0) alk, lower acyloxy-alpha-benzyl, and deltabutyrolactonyl; wherein alk is lower alkyl of 1 to 4 carbon atoms. 30. The compound of claim 14 of the formula (22) 22 and membrane-permeant fluorogenic derivatives thereof. 31. The compound of claim 1 of the formula (25) 25 and membrane-permeant fluorogenic derivatives thereof. 32. The compound of claim 27 wherein R γ is Cl and R x is H. (CCF2 or 76) . 33. The compound of claim 15 that is 34. The compound of claim 26 wherein R z and R " are acetoxymethyl; R x is H; and each R zl is acetyl. 35. The compound of claim 27 wherein R γ is H; and R x is methyl (CCFl 7a) . 36. The compound of claim 1 wherein said donor is selected from the group consisting of fluorescent europium and terbium complexes. 37. The compound of claim 36 wherein said donor is selected from the group consisting of europium and terbium tris- (bipyridine) cryptands and related dyes. 38. The compound of claim 1 wherein said quencher or acceptor is selected from the group consisting of phthalocyanines and related dyes. 39. The compound of claim 37 wherein said quencher or acceptor is selected from the group consisting of phthalocyanines and related dyes. 40. The compound of claim 39 that is 41. The compound of claim 10 wherein said coumarin is a compound of formula (III) : wherein m E is selected from the group consisting of H, OH, OR k , and NR 9 R h ; T is selected from the group consisting of O and NR k ; R a and R b are independently selected from the group consisting of an attachment point, H, halogen, and lower alkyl; R 9 , R h , and R k are independently selected from the group consisting of an attachment point, H, lower alkyl, and - CH 2 (CH 2 ) n a; R 1 is selected from the group consisting of an attachment point, H, halogen, lower alkyl, CN, CF 3 , phenyl, C0 2 H, and CONR 9 R h ; and a is selected from the group consisting of H or an attachment point; and membrane-permeant fluorogenic derivatives thereof. 42. The compound of claim 11 wherein said acceptor or quencher is a compound of formula (VIII) : vm wherein E is selected from the group consisting of H, OH, OR k , and R 9 R h ; G is selected from the group consisting of O and N * R 9 R h ; Q' is selected from the group consisting of 0, CH 2 , C(CH 3 ) 2 , and NR 9 ; R a , R b , R c , and R d are independently selected from the group consisting of an attachment point, H, halogen, and lower alkyl; R e is selected from the group consisting of an attachment point, H, lower alkyl, (CH 2 ) n C0 2 H, (CH 2 ) n CHaC0 2 H, CHa (CH 2 ) n C0 2 H, (CH 2 ) n C0a, CH=CHCOa; R 9 , R h , R k are independently selected from the group consisting of an attachment point, H, lower alkyl, and - CH 2 (CH 2 ) n a; n is an integer of from 0 to 5; a and a' are independently selected from the group consisting of H or an attachment point; and membrane-permeant fluorogenic derivatives thereof. 43. The compound of claim 42 wherein said donor is selected from the group consisting of coumarins of formulae II-VII, and membrane-permeant fluorogenic derivatives thereof, 44. The compound of claim 43 wherein Q' is C(CMe 2 ) 2 ; and G is O; and membrane-permeant fluorogenic derivatives thereof. 45. The compound of claim 43 selected from the group consisting of: 46. A method for determining whether a sample contains / S-lactamase activity comprising: contacting the sample with a compound of claim 1, which exhibits fluorescence resonance energy transfer when the compound is excited; exciting the compound; and determining the degree of fluorescence resonance energy transfer in the sample, whereby a degree of fluorescence resonance energy transfer that is lower than an expected amount indicates the presence of /3-lactamase activity. 47. The method of claim 1 for determining the amount of an enzyme in a sample wherein determining the degree of fluorescence resonance energy transfer in the sample comprises determining the degree at a first and second time after contacting the sample with the substrate, and determining the difference in the degree of fluorescence resonance energy transfer, whereby the difference in the degree of fluorescence resonance energy transfer reflects the amount of enzyme in the sample. 48. A recombinant nucleic acid molecule comprising expression control sequences adapted for function in a vertebrate cell and operably linked to a nucleotide sequence coding for the expression of a 3-lactamase. 49. The recombinant nucleic acid molecule of claim 48 wherein the nucleotide sequence encodes a class A β- lactamase. 50. The recombinant nucleic acid molecule of claim 49 further comprising mammalian Kozak sequences for the ribosome binding site. 51. The recombinant nucleic acid molecule of claim 50 wherein the nucleotide sequence is Sequence 3 or Sequence 4 of Figure 7. 52. The recombinant nucleic acid molecule of claim 49 wherein the nucleotide sequence is Sequence 1 or Sequence 5 of Figure 7. 53. The recombinant nucleic acid molecule of claim 48 wherein the expression control sequences are inducible expression control sequences. 54. The recombinant nucleic acid molecule of claim 48 wherein the expression control sequences are constitutively active expression control sequences. 55. The recombinant nucleic acid molecule of claim 48 wherein the expression control sequences include the c-fos promoter or the c-jun promoter. 56. The recombinant nucleic acid molecule of claim 48 wherein the expression control sequences include cyclic AMP-responsive elements, phorbol ester response elements, serum response element, or Nuclear Factor of Activated T-cells response elements. 57. The recombinant nucleic acid molecule of claim 48 wherein the expression control sequences are responsive to substances that modulate cell-surface receptors. 58. The recombinant nucleic acid molecule of claim 48 wherein the expression control sequences are responsive to substances that modulate intra-cellular receptors. 59. The recombinant nucleic acid molecule of claim 48 wherein the 3-lactamase comprises a signal sequence for extracellular secretion. 60. The recombinant nucleic acid molecule of claim 59 wherein the nucleotide sequence is Sequence 2 of Figure 7. 61. The recombinant nucleic acid molecule of claim 48 wherein the /.-lactamase is a cytosolic / S-lactamase. 62. The recombinant nucleic acid molecule of claim 61 wherein the nucleotide sequence is Sequence 1, Sequence 3, Sequence 4, or Sequence 5 of Fig. 7. 63. A recombinant nucleic acid molecule comprising expression control sequences adapted for function in a eukaryotic cell and operably linked to a nucleotide sequence coding for the expression of a cytosolic 3-lactamase. 64. The recombinant nucleic acid molecule of claim 63 wherein the nucleotide sequence encodes a class A β- lactamase. 65. The recombinant nucleic acid molecule of claim 64 wherein the nucleotide sequence is Sequence 1, Sequence 3, Sequence 4 or Sequence 5 of Figure 7. 66 . The recombinant nucleic acid molecule of claim 63 wherein the expression control sequences are inducible expression control sequences. 66. The recombinant nucleic acid molecule of claim 63 wherein the expression control sequences are constitutively active expression control sequences. 67. The recombinant nucleic acid molecule of claim 63 wherein the expression control sequences include the c-fos promoter or the c-jun promoter. 68. The recombinant nucleic acid molecule of claim 63 wherein the expression control sequences include cyclic AMP-responsive elements, phorbol ester response elements, serum response element, or Nuclear Factor of Activated T-cells response elements. 69. The recombinant nucleic acid molecule of claim 63 wherein the expression control sequences are responsive to substances that modulate cell-surface receptors. 70. The recombinant nucleic acid molecule of claim 63 wherein the expression control sequences are responsive to substances that modulate intra-cellular receptors. 71. A mammalian host cell transfected with a recombinant nucleic acid molecule further comprising expression control sequences adapted for function in a mammalian cell and operably linked to the nucleotide sequence coding for the expression of a /3-lactamase. 72. A method of determining the amount of β- lactamase activity in a cell comprising the steps of: providing a host cell transfected with a recombinant nucleic acid molecule comprising expression control sequences operatively linked to nucleic acid sequences coding for the expression of a /S-lactamase; contacting a sample comprising the cell or an extract of the cell or conditioned medium with a substrate for /8-lactamase; and determining the amount of substrate cleaved, whereby the amount of substrate cleaved is related to the amount of /8-lactamase activity. 73. A method for monitoring the expression of a gene operably linked to a set of expression control sequences comprising: providing a eukaryotic host cell transfected with a recombinant nucleic acid molecule comprising expression control sequences operatively linked to nucleic acid sequences coding for the expression of a /.-lactamase, except if the eukaryote is a fungus, wherein the /.-lactamase is a cytosolic /3-lactamase; contacting a sample comprising the cell or an extract of the cell or conditioned medium with a substrate for /S-lactamase; and determining the amount of substrate cleaved, whereby the amount of substrate cleaved is related to the amount of /8-lactamase activity. 74. The method of claim 73 wherein the eukaryotic cell is mammalian. 75. The method of claim 73 wherein the sample comprises an extract from the cell, with or without the conditioned medium. 76. The method of claim 73 wherein the sample comprises the cell. 77. The method of claim 73 wherein the substrate is a compound of claim 1 and the step of determining the amount of substrate cleaved comprises exciting the compound; and determining the degree of fluorescence resonance energy transfer in the sample, whereby a degree of fluorescence resonance energy transfer that is lower than an expected amount indicates the presence of /S-lactamase activity. 78. The method of claim 73 wherein the nucleotide sequence encodes a class A /8-lactamase. 79. The method of claim 78 wherein the nucleotide sequence is Sequence 1, Sequence 2, Sequence 3, Sequence 4 or Sequence 5 of Figure 7. 80. The method of claim 73 wherein the expression control sequences are inducible expression control sequences. 81. The method of claim 73 wherein the cell is an animal cell and the /3-lactamase is a cytosolic /3-lactamase. 82. A method for determining whether a test compound alters the expression of a gene operably linked to a set of expression control sequences comprising: providing a cell transfected with a recombinant nucleic acid construct comprising the expression control sequences operably linked to nucleic acid sequences coding for the expression of a / 3-lactamase except if the eukaryote is a fungus, wherein the / 3-lactamase is a cytosolic / 3-lactamase; contacting the cell with the test compound; contacting a sample comprising the cell or an extract of the cell with a /8-lactamase substrate; and determining the amount of substrate cleaved, whereby the amount of substrate cleaved is related to the amount of /3-lactamase activity. 83. The method of claim 82 wherein the eukaryotic host cell is mammalian. 84. The method of claim 82 wherein the sample comprises an extract from the cell. 85. The method of claim 82 comprises the cell. 86. The method of claim 82 wherein the substrate is a compound of claim 1 and the step of determining the amount of substrate cleaved comprises exciting the compound; and determining the degree of fluorescence resonance energy transfer in the sample, whereby a degree of fluorescence resonance energy transfer that is lower than an expected amount indicates the presence of /3-lactamase activity. 87. The method of claim 82 wherein the nucleotide sequence encodes a class A /3-lactamase. 88. The method of claim 87 wherein the nucleotide sequence is Sequence 1, Sequence 2, Sequence 3, Sequence 4 or Sequence 5 of Figure 7. 89. The method of claim 85 wherein the expression control sequences are inducible expression control sequences. 90. The method of claim 85 wherein the cell is an animal cell and the / 8-lactamase is a cytosolic / S-lactamase. 91. The method of claim 82 wherein the expression of /3-lactamase is operably regulated by activation, or inhibition of activation, of an intracellular receptor, a cell surface receptor, or a component of an intracellular signaling pathway. 92. A method of clonal selection comprising: providing cells transfected with a recombinant nucleic acid molecule comprising the expression control sequences operably linked to nucleic acid sequences coding for the expression of a cytosolic β-lactamase; contacting the cells with a substance that activates or inhibits the activation of the expression control sequences; contacting the cells with a compound of claim 9 which is converted into a substrate; determining whether substrate is cleaved within each individual cell, whereby cleavage reflects 3-lactamase activity; selecting and propagating those cells with a selected level of 3-lactamase activity. 93. The method of claim 92 further comprising the steps of: culturing selected cells in the absence of activator for a time sufficient for cleaved substrate to be substantially lost from the cells and for / 3-lactamse levels to return to unactivated levels; incubating the selected cells with a compound of claim 9 which is converted into a substrate; and selecting cells that have not substantially cleaved the substrate.

Inventors: TSIEN ROGER Y, ZLOKARNIK GREGOR, TSIEN, ROGER, Y., ZLOKARNIK, GREGOR
Applicants: TSIEN ROGER Y, UNIV CALIFORNIA, ZLOKARNIK GREGOR, THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, TSIEN, ROGER, Y., ZLOKARNIK, GREGOR
Application year: 1996
Main classification: C12Q
IPC codes: C07D463, C07D471, C07D501, C07D505, C12N5, C12N9, C12N15, C12P17, C12P35, C12Q1