EVA CONTAINING ION SELECTIVE MEMBRANES AND METHODS OF MAKING SAME

Abstract: Ion selective membranes and ion selective electrode constructions which include ethylene vinyl acetate (EVA), a reduced amount of plasticizer and an ion selective material. The ion selective membranes have a low crystallinity. In certain embodiments, the ion selective membranes may further include polyvinyl chloride (PVC), a salt or a combination thereof. The ion selective membrane can be incorporated into an ion selective electrode construction. Methods of manufacturing the ion selective membranes and ion selective electrode constructions are provided.

Description: EVA CONTAINING ION SELECTIVE MEMBRANES AND METHODS OF MAKING SAME Background 1. Field of the Invention The present invention relates generally to ion selective membranes and more specifically to EVA containing ion selective membranes having low crystallinity and methods of making same. 2. Discussion ofthe Related Art Ion selective sensors are commonly used in clinical, analytical and industrial chemical laboratories to provide rapid analysis ofthe concentration of a variety of analytes in solution. Conventionally, these ion selective sensors include an ion selective membrane which is in contact with the analyte containing solution. The potential difference between a reference electrode placed within the test solution and a contact electrode in communication with the ion selective membrane is used to measure the concentration ofthe analyte in the test solution. Ion selective membranes often include a polymeric material, an external plasticizer and an ion sensing component. For example, U.S. Patent No. 4,214,968 and European Patent No. 0 498 572 A2 disclose such membranes wherein the polymeric material is polyvinyl chloride (PVC). These membranes include at least about twice as much plasticizer as polymer by weight. However, such relatively high amounts of plasticizer can reduce the lifetime ofthe ion selective sensor due to leaching ofthe plasticizer into the analyte containing solution. Anal. Chim. Acta 273, 153(1993) and Talanta 38, 929(1991) each disclose ion selective membranes including ethylene vinylacetate copolymer (EVA) and an external plasticizer. The amount of plasticizer within the membrane is nearly triple the amount of EVA within the membrane, so leaching ofthe plasticizer into the analyte solution can result in a reduced lifetime of these membranes. Some ion selective membranes have been produced which do not include an external plasticizer. U.S. Patent Nos. 4,981,570 and 5,078,856 disclose ion selective membranes which comprise PVC and a modified form of EVA. This modified EVA serves as an internal plasticizer and contains oxygen atoms in the backbone ofthe polymer. However, the introduction of oxygen atoms into the EVA results in membranes which can be both relatively expensive and complex to manufacture. An ion-exchange membrane containing EVA treated with sulfonic acid is disclosed in Materiale Plastice 25, 177(1988). The acid treatment creates functionalized EVA containing sulfonate groups, and the resulting membranes have increased ion transport within the membrane. However, since these membranes have a relatively small amount of vinyl acetate and are cross-linked, they can be too stiff to be used as ion selective membranes in ion selective sensors. Thus, existing ion selective membranes often are relatively complex to produce, have relatively short lifetimes or both. Therefore, it remains a challenge in the art to provide ion selective membranes of relatively simple design which contains a relatively low level of materials which can leach into the analyte solution. Accordingly, it is a general purpose ofthe present invention to provide a membrane capable of having a relatively long lifetime and which is relatively easy to prepare. Summary of The Invention Therefore, it is an object ofthe present invention to provide an ion selective membrane which includes a relatively low concentration of plasticizer or substantially no plasticizer. It is another object ofthe present invention to provide an ion selective membrane in accordance with the preceding object, which is relatively easy to prepare. It is a further object ofthe present invention to provide an ion selective membrane in accordance with the preceding objects which has a comparatively long life. It is yet a further object ofthe present invention to provide an ion selective membrane which has low crystallinity. It is yet another object ofthe present invention to provide a method of making an ion selective electrode construction which includes an ion selective membrane in accordance with the preceding objects. It is still another object ofthe present invention to provide an ion selective electrode construction which includes an ion selective membrane in accordance with the preceding objects. Accordingly, one embodiment ofthe present invention includes an ion selective membrane which comprises an ethylene vinylacetate (EVA) polymer. The EVA polymer includes from about 30 weight percent to about 75 weight percent vinylacetate monomer, from about 0% to about 20% plasticizer by weight and at least one ion selective material. The membrane is from about 0% crystalline to about 10% crystalline. Another embodiment ofthe present invention includes an ion selective membrane comprised of ethylene monomers, vinylacetate monomers and monomers of at least one additional vinyl species having a charged site or a polar group. The polymer includes from about 35 weight percent to about 55 weight percent vinyl acetate monomer and is substantially devoid of plasticizer. The membrane additionally includes at least one ion selective material. The membrane is from about 0% crystalline to about 10% crystalline. In a further embodiment ofthe present invention, an ion selective electrode construction is provided which includes at least one electrode and an ion selective membrane which is operatively positioned with respect to the electrode. The membrane comprises an ethylene vinylacetate polymer which includes from about 30 weight percent to about 75 weight percent vinylacetate monomer and from 0 per cent to about 20 per cent by weight plasticizer. The membrane further includes at least one ion selective material. The membrane is from about 0% crystalline to about 10% crystalline. In yet another embodiment ofthe present invention, a method of forming an ion selective electrode construction for use in ion selective electrode determinations is provided. The method includes providing a reference electrode and positioning an ion selective membrane in operative position over the reference electrode. The membrane comprises an ethylene vinylacetate polymer which includes from about 30 weight percent to about 75 weight percent vinylacetate monomer and about 0% to about 20%) plasticizer by weight. The membrane further comprises at least one ion selective material. The membrane is from about 0% crystalline to about 10%> crystalline. It is a feature ofthe present invention that ion selective membranes can be formed having comparatively long lifetimes. Moreover, in certain embodiments ofthe present invention, having a reduced amount of plasticizer in an ion selective membrane results in improved water uptake characteristics. Brief Description of the Drawings The objects, advantages and features ofthe present invention will be more clearly appreciated from the following detailed description when taken in conjunction with the accompanying drawings, in which: FIG. 1 is a cross-sectional view of an ion selective membrane in accordance with the present invention; and FIG. 2 is a cross-sectional view of an ion selective electrode construction in accordance with the present invention. Detailed Description The present invention relates to ion selective membranes and ion selective electrode constructions as shown in FIGS. 1 and 2, respectively. FIG. 1 depicts an ion selective membrane 10 which, according to the present invention, has low crystallinity. "Low crystallinity" is herein meant to refer to crystallinity from about 0%> to about 10%), more preferably from about 0%> to about 5% and most preferably from about 0%> to about 3%. Crystallinity as used herein corresponds to polymer crystallinity as measured using x-ray powder diffraction such as disclosed in Makrmol. Chemie 50, 98(1961) and J. Appl.Polym.Sci. l±, 173 (1970). As known to those skilled in the art, there is a general correlation between the crystallinity of a polymeric material and the stiffness ofthe polymeric material. For ion selective membranes according to the present invention, the stiffness of membrane 10 has particularly advantageous values when membrane 10 has a crystallinity below about 10%. Membrane 10 may have any stiffness so long as membrane 10 has low crystallinity. Membrane 10 should have a stiffness at least as great as that of a membrane comprised of 33% PVC and 66% plasticizer (NPOE or DOA). Preferably, membrane 10 has a G-modulus of from about 0.1 MegaPascal to about 2.0 MegaPascals, more preferably from about 0.3 MegaPascals to about 1.0 MegaPascals, and most preferably about 0.6 MegaPascals as measured using the DIN 53 455 method as disclosed in Kunststoffe 57, 321 (1967). Typically, membrane 10 is sensitive to ions at concentration levels as disclosed in Anal. Sci. 4, 547(1988); Anal. Chim. Acta 255, 35 (1991); J. Chem. Soc. Far. I_2 1179(1986); Clinical Chemistry 32, 1448 (1986); or SPIE 1510. 118(1991). For example, membrane 10 may be sensitive to concentration levels of at least about: 10 "58 M for NH 4 + ; 10 "74 M for Ca 2+ ; 10 "53 M for Li + ; 10' 12 M for H + ; 10 "5 M for Mg 2+ and 10 -" M for Na + . Although particular ion selectivities have been disclosed herein, membrane 10 may have any ion selectivity so long as membrane 10 has low crystallinity. Conventionally, the ion selectivity of membrane 10 is measured by two methods. In the separate solution method, a sensor is placed in many different solutions sequentially, and the potential in each solution is measured. Each solution contains only one type of ion and counter ion. The Nicolsky equation (given below) is then applied to the measured potentials ofthe known concentrations to calculate the selectivity coefficients. The slope is assumed to be constant. In the method of mixed solutions, both ions of interest and interfering ions are present. The concentration of one of these ions is varied while the other is held constant. The response is fit to the Nicolsky equation to give the selectivity coefficient. The Nicolsky equation is given by: E=E c + (RT/z,F) ln([i] + k 1J [jr zJ ), where E c is a constant, R is the gas constant, T is temperature in units of Kelvin, i is the ion of interest, j is the interfering ion, z, is the charge ofthe ion of interest, z, is the charge on the interfering ion, k υ is the selectivity coefficient, F is the Faraday constant, and (RT/z,F) is the slope ofthe membrane in units of millivolts (mV) per natural log of analyte concentration. While the slope of membrane 10 may have any value so long as membrane 10 has low crystallinity, membrane 10 preferably has a slope of at least about 45/z, mV per decade of analyte concentration, more preferably at least about 50/z, mV per decade of analyte concentration and most preferably at least about 55/z, mV per decade of analyte concentration. Fig. 1 shows an embodiment of membrane 10 in which it is disk-like in shape. For such embodiments, membrane 10 preferably has a radius of from approximately 0.2 cm to approximately 0.4 cm. In certain embodiments ofthe present invention in which membrane 10 is used in a free standing electrode, membrane 10 preferably has a thickness from approximately 100 micrometers to approximately 300 micrometers. For certain embodiments ofthe present invention in which membrane 10 is a planar electrode, membrane 10 preferably has a thickness from about 10 micrometers to about 100 micrometers. Although certain dimensions of membrane 10 have been described herein, it is to be understood that membrane 10 may have any shape so long as it has low crystallinity. The electrical resistivity of membrane 10 may be any so long as membrane 10 has low crystallinity. For membranes having a thickness of about 100 micrometers and an area of about 0.32 cm 2 , the electrical resistivity is typically from about IO 4 Ohms to about 5x10 8 Ohms, more preferably from about 10 4 Ohms to about 2xl0 7 Ohms. Membrane 10 may have any lifetime so long as it has low crystallinity. Preferably, membrane 10 has a lifetime of at least 10 days with a slope of at least 85% ofthe theoretical value (based on the Nicolsky equation), more preferably at least 15 days with a slope of at least 85%) ofthe theoretical value, and most preferably at least 20 days with a slope of at least 85%> of the theoretical value as measured by an accelerated aging test. The accelerated aging test includes storing the material of which the membrane is comprised under pressure in water at 115°C, preparing a membrane from the material at least every two days, and testing the slope of the membrane at room temperature. According to the present invention, membrane 10 comprises an EVA copolymer and at least one ion selective material. In certain embodiments, membrane 10 may also include a plasticizer, PVC, a salt or a combination thereof. It is to be appreciated that membrane 10 may include various combinations of these or other materials appropriate for use in ion selective membranes so long as membrane 10 has low crystallinity. By "copolymer" it is herein meant to refer to a polymerization product containing more than one monomer unit. For example, the monomer units of copolymers may be arranged such that the copolymers are selected from an illustrative and nonlimiting list which includes alternating copolymers, random copolymers, block copolymers and graft copolymers. In addition, copolymers according to the present invention may be arranged such that the copolymers include, but are not limited to, linear copolymers, branched copolymers (including star copolymers, comb copolymers, ladder copolymers and semiladder copolymers) and network copolymers. While certain types of copolymers have been disclosed herein, these lists are not limiting and any copolymer may be used according to the present invention so long as membrane 10 has low crystallinity. According to the present invention, membrane 10 may include a normal EVA copolymer, a modified EVA copolymer, a functionalized EVA copolymer or a modified, functionalized EVA copolymer. Preferably, membrane 10 includes a normal EVA copolymer, a modified EVA copolymer or a functionalized EVA copolymer. More preferably, membrane 10 includes a normal EVA copolymer. By "normal EVA copolymer" it is herein meant to refer to an EVA copolymer which has substantially all carbon atoms in its backbone and is comprised of substantially all ethylene and vinyl acetate monomer units. "Modified EVA copolymers" herein denote EVA copolymers which include noncarbon atoms in their backbone structures such as, for example, O, S, N or atoms. A "functionalized EVA copolymer" herein refers to an EVA copolymer which includes functional groups other than those of ethylene and vinyl acetate. A "modified, functionalized EVA copolymer" herein refers to an EVA copolymer which is both a modified EVA copolymer and a functionalized EVA copolymer as defined herein. Although certain types of EVA copolymers have been disclosed herein it is to be appreciated that any EVA copolymer may be used according to the present invention so long as membrane 10 has low crystallinity. The dielectric constant of EVA usually increases as the weight percent of vinyl acetate in the EVA increases as disclosed in Vysokomol. Soyed. A15. 1382 (1972) and Kunstsqffe 57, 321 (1967). Preferably, the dielectric constant of membrane 10, which comprises normal EVA, is from about 2.8 to about 4.5, more preferably from about 3 to about 4.5 and most preferably from about 3.3 to about 4.5. For embodiments ofthe present invention which include modified EVA and/or functionalized EVA, the dielectric constant of membrane 10 may be as high as about 30 or as low as about 2.8. However, it is to be appreciated that the dielectric constant of membrane 10 may have any value so long as membrane 10 has low crystallinity. The molecular weight of EVA copolymers appropriate for use according to the present invention is preferably from about 40,000 Daltons to about 500,000 Daltons, more preferably from about 100,000 Daltons to about 400,000 Daltons and most preferably about 250,000 Daltons as measured using the methods disclosed in Br. J. Polym. 1, 225 (1969). However, it is to be appreciated that EVA copolymers having other molecular weights may also be used so long as membrane 10 has low crystallinity. EVA copolymers appropriate for use in the present invention may have any second order transition temperature so long as membrane 10 has low crystallinity. Preferably, EVA copolymers have a second order transition temperature of from about -15°C to about-45°C, more preferably from about -15°C to about -35°C and most preferably from about -20°C to about -30 °C as measured according to DIN 53 513 or as disclosed in Kautschuk u. Gummi Kunststoffe 22, 116 (1969). For embodiments of membrane 10 which include a plasticizer, EVA copolymers preferably include from about 30 weight percent to about 75 weight percent vinyl acetate monomers, more preferably from about 40 weight percent to about 50 weight percent vinyl acetate monomers, and most preferably about 45 weight percent vinyl acetate monomers. However, these ranges should not be construed as limiting. The weight percent of vinyl acetate for EVA used in membrane 10 for such embodiments may be any so long as membrane 10 has low crystallinity. According to embodiments ofthe present invention in which membrane 10 includes about 0% plasticizer by weight (i.e., membrane 10 is "substantially devoid of plasticizer"), EVA copolymers preferably include a weight percentage of vinyl acetate from about 30 percent to about 55 percent, more preferably from about 40 percent to about 50 percent vinyl acetate monomer, and most preferably from about 45 percent vinyl acetate monomer. However, it is to be appreciated that EVA copolymers appropriate for use in these embodiments may comprise any mole percentage of vinyl acetate so long as membrane 10 has low crystallinity. It should be noted that having a reduced amount of plasticizer in membrane 10 usually increases the adhesive properties thereof relative to most known membranes used in ion selective electrodes. For certain embodiments, the increased adhesion of membrane 10 may be advantageous such as, for example, when membrane 10 is used in a planar ion selective device. Other embodiments for which relatively high adhesion for membrane 10 is advantageous are known to those skilled in the art and are intended to be within the scope ofthe present invention. EVA copolymers according to the present invention may be synthesized using techniques such as disclosed in Br. Polym. J. 1, 225 (1969), Kautschuk u. Gummi Kumstoffe 22, 116(1969), U.S. Patent No. 3,325,460 and German Patent No. 1,126,613. These methods include, for example, high pressure radical reaction of vinyl acetate and ethylene (at pressures from about 1500 to about 2000 atmospheres), moderate pressure radical polymerization of vinyl acetate and ethylene (at pressures from about 100 to about 500 atmospheres) or emulsion polymerization. High pressure radical polymerization typically produces EVA having up to about 40 % vinyl acetate by weight. Medium pressure polymerization usually results in EVA which has from about 30%) to about 100%> vinyl acetate by weight. Emulsion polymerization can produce EVA having an amount of vinyl acetate which covers the whole range, but the product can be highly branched or even cross-linked. Preferably, medium pressure polymerization is used to synthesize EVA. Other methods of synthesizing EVA are known to those skilled in the art and are intended to be within the scope ofthe present invention. Such techniques are limited only in that, when the resulting EVA is incorporated into membrane 10, membrane 10 has low crystallinity. Methods of synthesizing modified and/or functionalized EVA copolymers appropriate for use in the present invention are known to those skilled in the art and are intended to be within the scope ofthe present invention. For example, incoφoration of non- vinyl acetate, vinyl monomers and non-ethylene monomers can be accomplished by forming copolymers according to standard methods. Carbon monoxide (CO) can be polymerized with ethylene and vinyl acetate such as disclosed in Vysokomol. Soyed.AH, 2585 (1969). Using these methods, copolymers including ethylene, vinyl acetate and carbon monoxide with up to 70 mole percent carbon monoxide can be produced without substantially changing the crystallinity or elasticity ofthe material. J.Polym.Sci.All, 9, 3083 (1971) discloses methods of polymerizing a variety of compounds with ethylene and vinyl acetate such as, for example, vinyl chloride, vinyl fluoride, propylene and hexene- 1. Any of these methods and copolymers may be used according to the present invention so long as membrane 10 has low crystallinity. However, it should be noted that the crystallinity ofthe copolymer depends upon the monomer polymerized with ethylene and vinyl acetate. Therefore, when vinyl chloride and vinyl fluoride are incorporated into the copolymer, up to about 50 mole percent ofthe additional monomer may be included in the copolymer. About 31 mole percent of propylene may be used, and the amount of hexene- 1 used may be based on the fact that hexene- 1 has substantially the same crystallinity as vinyl acetate. It should be further noted that, in certain embodiments, incoφorating these monomers into the copolymer may change the dielectric constant ofthe overall polymer. Vysokomol. Soyed.AH, 2585 (1969) discloses compounds that can be copolymerized with ethylene to form copolymers. These compounds include vinylene carbonate, maleic anhydride and organic esters of phosphinic acid. Typically, the monomers which are polymerized with ethylene and vinyl acetate are capable of resulting in a copolymer with a lower crystallinity that EVA. However, it should be noted that not all these additives cause a substantial change to the crystallinity ofthe copolymer. Accordingly, for those monomers which do not assist in decreasing the crystallinity of the overall material, they are typically be present in amounts of less than about 5% ofthe overall material by weight. However, it is to be appreciated that the amount ofthe monomer polymerized with ethylene and vinyl acetate is limited only in that, when membrane 10 comprises the resulting copolymer, membrane 10 has low crystallinity. Other copolymers are known to those skilled in the art and include monomer units of, for example, 4-ethylidene-2-nobornene, 5-ethylidene-2-nobornene, hexaydro- dimethanonaphthalene, N-methylacryalamide cyanurate, methylmethacrylate, methylene succinic acid, and ethylene oxide with S-trioxone. Many ofthe monomers which may be used to use copolymers with ethylene and vinyl acetate are capable of introducing heteroatoms (i.e., non- carbon atoms) into the backbone ofthe copolymer. Ionomers appropriate for use in the present invention can also be made from EVA synthetic methods using copolymerization in which charge groups are introduced as pendant side chains as described in Kunststojfe 57, 321 (1967). For such embodiments, the copolymer preferably includes from about 0.001%> to about 5% ofthe ionized charge group by weight, more preferably from about 0.1 %> to about 4% and most preferably 0.5%> to about 2%>. However, it is to be understood that the amount ofthe ionized charged group within copolymers which may be included in membrane 10 are limited only in that membrane 10 should have low crystallinity. According to the present invention, membrane 10 may include EVA which has PVC grafted thereto. Methods of synthesizing such copolymers are known to those skilled in the art and include those disclosed in, for example, Br. Polym. J. _ . , 225, (1969). Typically, such copolymers include up to 50% grafted PVC by weight, more preferably less than about 10%). EVA copolymers appropriate for use in the present invention may also be synthesized by hydrolyzing the acetate group in vinyl acetate to yield vinyl alcohol. It should be noted that, as disclosed in J. Polym. Sci. Ai, 3083(1971), essentially all forms of ethylene vinyl alcohol have some crystallinity. Accordingly, according to the present invention, copolymers containing vinyl alcohol monomers preferably include less than about 20% vinyl alcohol by weight. Ion selective materials appropriate for use according to the present invention should make membrane 10 permselective. Such materials are capable of complexing a desired ion and extracting an ion of one charge without extracting an ion of opposite charge. These ion selective materials include, for example, nondissociable, ion-specific ligands, i.e., ionophores, or electrically charged liquid ion-exchangers. Ion selective materials are known to those skilled in the art and, in the present invention, are limited in their type and mole percent of membrane 10 only by the fact that membrane 10 and should have low crystallinity. Preferably, membrane 10 comprises from about 0.05 percent to about 10 percent of ion selective material by weight, more preferably from about 1 percent to about 6 percent ion selective material by weight, most preferably from about 1 percent to about 3 percent ion selective material by weight. In certain embodiments, more than one ion selective material may be incoφorated into membrane 10. In embodiments ofthe present invention which include more than one ion selective material, membrane 10 preferably comprises from about 0.05 percent to about 10 percent ion selective material by weight, more preferably from about 1 percent to about 6 percent by weight and most preferably from about 1 percent to about 3 percent by weight. However, it is to be understood that membrane 10 may comprise any amount of ion selective material and any number of ion selective materials so long as membrane 10 has low crystallinity. Ion selective materials according to the present invention may be any of those known to be selective towards a particular ion to be analyzed. An illustrative and nonlimiting list of such ion selective materials includes: for potassium, valinomycin, bis (benzo- 15-crown-5)-4- ylmethyl)pimelate dicyclohexano-18-crown-6, dibenzo-18-crown-6, tetraphenyl borate, tetrakis (p-chlorophenyl) borate, cyclopolyethers, tetralactones macrolide actins (monactin, nonactin, dinactin, trinactin), the enniatin group (enniatin A, B), cyclohexadepsipeptides, gramicidine, nigericin, dianemycin, nystatin, monensin, esters of monensin, antamanide, alamethicin (cyclic polypeptieds); for calcium, ETHlOOl (as disclosed in Anal. Chem. 53, 1970(1981), ETH129, A23187 (Fluka of Buchs, Switzerland), ETH 5234 (Fluka of Buchs, Switzerland), bis (didecylphosphate), bis (4-octylphenylphosphate), bis (4-(l, 1, 3, 3 -tetramethy Ibutyl)) phenylphosphate, tetracosamethylcyclododecasiloxane, N, N'-di ((11 -ethoxycarbonyl) undecyl)- N,N'4, 5-tetramethyl-3, 6-dioxaoctane diamide, antibiotic A-23187 (as disclosed in Ann. Rev. Biochem. 45_, 501(1976)); for hydrogen, ETH1907 (Fluka of Buchs, Switzerland), ETH1778 (Fluka of Buchs, Switzerland), tridodecylamine, ETH 1907, N-methyl n-octadecyl (1 -methyl, 2- hydroxy, 2-phenyl) ethylamine, N-octadecyl 3-hydroxy N propylamine, N, N' bis (octadecyl ethylene amine), p-octadecyloxy-m-chlorophenylhydrazonemeso oxalonitrile; for sodium, monensin, ETH227 (Fluka of Buchs, Switzerland), ETH157 (Fluka of Buchs, Switzerland), ETH2120 (Fluka of Buchs, Switzerland), ETH4120 (Fluka of Buchs, Switzerland), ETH227, ETH157, NAS π . lg , N, N\ N"-triheptyl-N,N\ N"-trimethyl-4,4', 4 ,, -propylidintris-(3- oxabutyramide), 4-octadecanoyloxymethyl-N, N, N', N'-tetracyclohexyl-l, 2- pheny lenedioxy diacetamide, bis[(12-crown-4) methyl] dodecylmethylmalonate, ETH 149, ETH1810, cyclopolyethers; for lithium, N, N'-diheptyl-N, N', 5, 5-(tetramethyl-3, 7- dioxononanediamide), ETH 149 (Fluka of Buchs, Switzerland), ETH 1644 (Fluka of Buchs, Switzerland), ETH 1810 (Fluka of Buchs, Switzerland), ETH2137 (Fluka of Buchs, Switzerland), 6,6-dibenzyl-14-crown-4; 6,6-dibenzyl-l, 4, 8, 11 -tetraoxa-cyclotetradecane (Fluka of Buchs, Switzerland), 6[2-(diethlphosphonoox)ethyl] 6-dodecyl-14-crown-4 (Fluka of Buchs, Switzerland), 12-chrown-4, 6, 6-dibenzyl-14 chrown-4, cyclopolyethers, dodecylmethyl 14-crown-4; for chloride, tridodecyl ammonium salt, 5, 10, 15, 20-tetraphenyl-21H, 23H-poφhin manganese (111) chloride (Fluka of Buchs, Switzerland), quaternary ammonium chloride and tributyl tin chloride; for rubidium, cyclopolyethers; for cesium, cyclopolyethers; for HCO 3 " -, trifluoroacetyl-p-alkylbenzenes; for NH 4 + , nonactin, monactin; for NO 3 ", tridodecylammonium chloride; and for Mg 2+ ,ETH 7025 (Fluka of Buchs, Switzerland or as disclosed in Anal. Chem. 341. 727(1991)), ETH1117 (Fluka Buchs of Switzerland), ETH5214 (Fluka Buchs of Switzerland), ETH4030 (Fluka of Buchs, Switzerland) and ETH5214. Preferably, the ion selective materials include: for potassium, valinamycin; for calcium, ETHlOOl ; for NH 4 + , nonactin; for hydrogen, triododecylamine; for NO 3 ~ , tridodecylammonium chloride; for Cl", tridodecyl ammonium salt; and for sodium, ETH2120 (Fluka of Buchs, Switzerland). All ETH compounds are as disclosed in Anal. Sci. 4, 547 (1988) unless otherwise noted. A "plasticizer" as used herein refers to a material which can be added to membrane 10 to reduce the stiffness thereof. Typically, such plasticizers comprise polymeric materials, organic solvents or oils which are generally known in the art as external plasticizers (i.e., not chemically incoφorated into the backbone ofthe EVA copolymer). An illustrative and nonlimiting list of plasticizers appropriate for use in the present invention includes dioctyl adipate (DO A) nitrophenyl octylether (NPOE), dimethyl phthalate, dioctylphenyl-phosphonate, dibutyl phthalate, hexamethyl phosphoramide, dibutyl adipate, diundecyl phthalate, and dioctyl sebacate (DOS). Other plasticizers are known to those skilled in the art and may be used according to the present invention so long as membrane 10 has low crystallinity. For certain embodiments, a plasticizer can be selected based on the ion for which membrane 10 is selective. In such cases, the plasticizer may be selected by performing a test of ion selectivity and slope of response to the ion of interest. For example, when membrane 10 is intended to be selective for potassium, DOA and DOS are preferably used as plasticizers, and when membrane 10 is designed to be selective towards calcium, NPOE is the preferred plasticizer. Other plasticizers appropriate for use in membrane 10 when designed to be selective for certain ions are known to those skilled in the art and are meant to be within the scope ofthe present invention. Moreover, a plasticizer need not be selected based on the ion toward which membrane 10 is selective. It is to be appreciated that the use of plasticizers in ion selective membranes according to the present invention can result in ion selective membranes which have relatively long lifetimes as measured according to methods disclosed herein. In some embodiments, it may be desirable to improve the ion transference characteristics and permselectivity of membrane 10 by adding a salt thereto. In embodiments ofthe present invention which include salts, the salt preferably has a mole percent of membrane 10 from about 10% to 80%) ofthe mole percent ofthe ion selective material, more preferably from about 33%> to 66%o ofthe mole percent ofthe ion selective material and most preferably about 50% > ofthe mole percent ofthe ion selective material. In certain embodiments, the added salt may also serve as an integral part ofthe ion selective material. For such embodiments, the amount of salt within membrane 10 should not exceed the amount of ion selective material included within membrane 10 as disclosed elsewhere herein. Preferably, the salt is KB(C 6 H 4 X) 4 where X is H, F or CF 3 . For all embodiments, the amount of salt included within membrane 10 is limited only in that membrane 10 should have low crystallinity. Addition of salts to membrane 10 may be carried out by several methods. In certain embodiments, such salts may act as ion selective materials. According to one method, membrane 10 is cast as a free-standing material which is soaked in an aqueous solution comprising the salt to be added. Typically, such an aqueous solution has a salt concentration from about ImM to about IM. Preferably, the polymer and the salt to be added are dissolved in a solvent. The salt can be at the desired mole percent relative to the polymer. The dissolved polymer and salt may then be cast. The materials of membrane 10 may be drop cast or spin cast from a solution including the salt. While some methods of incoφorating salts into membrane 10 have been disclosed herein, other methods are known to those skilled in the art and are within the scope ofthe present invention. Ion selective membranes in accordance with the present invention may be made according to any method so long as membrane 10 has low crystallinity. In one embodiment of the present invention, membrane 10 is prepared by dissolving an EVA copolymer in a first solvent. A solution containing the ion selective material(s) and a second solvent is then added to the EVA copolymer containing solution. The order of mixing ofthe above-mentioned solutions may be reversed in some embodiments. In addition, the EVA copolymer and ion selective material(s) may be dissolved within the same solvent. The resulting solution including the EVA copolymer and the ion selective material(s) is molded according to standard methods to form membrane 10 such as, for example, standard solvent casting methods. It is to be appreciated that while certain methods of forming a membrane according to the present invention have been disclosed herein, other methods of forming membrane 10 will be apparent to those skilled in the art and are intended to be within the scope ofthe present invention. Solvents appropriate for use in the present invention are limited only to the extent that they should be capable of dissolving the EVA copolymer and other components of membrane 10 without changing the chemical structure ofthe EVA copolymer or other materials of which membrane 10 is comprised. Typical solvents include tetrahydrofuran (THF), carbon tetrachloride, chloroform, methylene chloride, p-xylene and toluene. Preferably, the solvent is THF. Other solvents having the aforementioned properties , such as halogenated hydrocarbons and aromatic solvents, may also be used and are known to those skilled in the art. For embodiments ofthe present invention which incoφorate PVC, a plasticizer or other materials appropriate for use in membrane 10, these materials may first be dissolved in a solvent as described above and subsequently mixed with the EVA/ ion selective material(s) solution. Alternatively, the order of mixing may be reversed. In addition, all the materials including the EVA copolymer and ion selective material(s) may be dissolved within the same solvent. The resulting solution may be molded as given above to form membrane 10, or other methods of forming such membranes which are known to those skilled in the art may be used. FIG. 2 depicts an ion selective electrode cell construction 20 in accordance with the present invention. Ion selective electrode construction 20 includes ion selective membrane 10, analyte solution 12, reference electrode 14, and ion-selective electrode 16. Reference electrode 14 includes saturated solution 18, junction 11, bridge electrolyte 13 and capillary 15. Ion selective electrode 16 includes membrane 10, contact electrode 17 and internal solution 19. Reference electrode 14 preferably provides precision to the voltage stability to less than 100 microvolts which can allow the precision ofthe concentration measurement to be about 1%. Such reference electrodes include the Ag/AgCl electrode, the calomel electrode, Ag/Ag oxide electrodes and other oxide electrodes. It should be noted that, when using an oxide electrode with a limited volume electrode reservoir, a pH buffer solution having a value near that ofthe analyte solution 12 should be used. Such buffer solutions are known to those skilled in the art and are intended to be within the scope ofthe present invention. Moreover, while one particular configuration of construction 20 is depicted in Fig. 2, other constructions appropriate for use in the present invention will be apparent to those skilled in the art and are intended to be within the scope ofthe present invention. These components may be any arrangement so long as ion selective electrode construction 20 operates to measure to the concentration ofthe analyte in analyte solution 12. Inner reference electrode 17 may be, for example, an Ag, Pt, C, Iridium oxide or Ag/AgCl electrode, or the insulator of a field effect type electrode such as silicon nitride, silicon dioxide or alumina. In addition, inner reference electrode 17 may contact a solid electrolyte, a polymer electrolyte or a polymer membrane disposed between inner reference electrode 17 and membrane 10. Moreover, inner reference membrane 17 may be a polymer with a redox couple attached thereto. Furthermore, inner reference electrode 17 may be a polymer with a redox couple blended (mixed) therein. Inner reference electrode 17 may also be a planar fabricated electrode such as disclosed in U.S. Patent No. 4,734,184. Other embodiments of inner electrode 17 will be apparent to those skilled in the art and are intended to be within the scope ofthe present invention. Analyte solution 12 includes the ion to be detected (i.e., the analyte) and a solvent. Solvents which can be used are limited only in that they should dissolve the analyte, not dissolve membrane 10 and not interfere with the measurement ofthe analyte concentration or chemically react with membrane 10 or any other portion of construction 20. Typically, the solvent includes water. Such solvents include, but are not limited to, water, blood, urine, and other aqueous solutions. In certain embodiments, it is advantageous to buffer analyte solution 14 to have a constant ionic strength. When analyte solution 12 is buffered in calibration solutions, it is done to match the ionic strength ofthe sample. For example, if analyte solution 12 comprises blood, the solution would typically be buffered to 0.15M. In river water, the ionic strength may be buffered to be from about 10 "4 to about 10 "3 M. For Ca 2+ electrodes, analyte solution 12 is often buffered to 0.33M. Buffering a solution is usually accomplished by adding a salt to analyte solution 12 to buffer the ionic strength. The ionic strength of an ion in solution is given by where μ is the ionic strength ofthe ion used to buffer the solution, Cj is the concentration ofthe ion used to buffer the solution and z,- is the charge on the ion used to buffer the solution. Such salts are limited only in that they should not interfere with the measurement ofthe concentration ofthe analyte. The particular salt used depends upon the analyte. For example, for measurements of Ca 2+ and K + in blood, the salt is often NaCl. To measure sodium, potassium chloride may be used. For Cl " measurements, HCO 3 " is typically used, and for H + many different buffer salts may be used. The particular buffer salt used is limited only in that it should allow an accurate measurement ofthe concentration of analyte in analyte solution 12. Certain embodiments ofthe present invention include internal solution 19. Typically, internal solution 19 includes a salt and a solvent. Salts which may be used in internal solution 19 include, but are not limited to, NaCl, KCI and CaCl which may be used with Ag/AgCl electrodes or calomel electrodes. Internal solution 19 should include a concentration of from about O.lmM to about 0.5M ofthe analyte. Preferably, this ion is in the form ofthe chloride salt. The solvent used for internal solution 19 should not interfere with the measurement ofthe analyte or alter the chemical structure of membrane 10 or any other portion of construction 20. Such solvents are typically polar solvents including aqueous solutions. Preferably, the solvent is water. It is to be appreciated that ion selective membranes in accordance with the present invention can be incoφorated into field effect transistors. Such transistors typically would include a layer of semiconductor material, a pair of diffusion regions, another diffusion region having a doping characteristic different from the pair of diffusion regions, an electrically insulating material provided on the semiconductor material, an ion selective membrane separated from the diffusion regions by the insulating material, and an electrically insulating encapsulant sealing the transistor. The encapsulant seals the transistor from a sample region while leaving the ion selective membrane exposed to a particular sample. Other such chemical-responsive transducers capable of having an ion selective membrane in accordance with the present invention incoφorated therein will be apparent to those of skill in the art and are intended to be within the scope ofthe present invention. Example 1 A membrane according the present invention including 96.1% EVA(45), 3 % valinomycin and 0.3 % KB(C 6 H 4 C1) 4 by weight was prepared as follows. 6.5 ml of EVA (45) (Item number 784 obtained from Scientific Polymer Products, Ontario, NY 14519, molecular weight of 260,000 as measured by gel permeation chromatography, melt index of 2-5 measured according to ASTM D 1238-62 T, softening point of 182° C) dissolved in distilled tetrahydrofuran (THF) (obtained from Aldrich Chemical Co. located in Milwaukee, WI) to a concentration of 30 mg\ml was mixed with 3 ml of valinomycin (purchased from Sigma located in St. Louis, Missouri) dissolved in THF to a concentration of 2 mg\ml. 0.6 ml of KB(C 6 H 4 C1) 4 (purchased from Fluka of Buchs, Switzerland) dissolved in THF to a concentration of 1 mg\ml was added to the EVA (45)/valinomycin solution. This EVA/45 valinomycin/KB(C 6 H 4 Cl) 4 solution was mixed with a magnetic stir bar for one hour at room temperature under atmospheric pressure. The solution was then filtered to remove impurities. About 3-5%> ofthe mass was lost during the filtration, and the filtered solutions had concentrations of about 35 mg/ml. The solution was then cast in a Teflon mold having a 3.82 centimeter diameter according to the method disclosed in J. Chem Educ. 51, 541 (1974). The membrane was dried for 48 hours and smaller diameter circles were then cut out ofthe membrane and then mounted in Phillips-type electrode body for testing as ion selective electrodes using the construction depicted in FIG.2. An internal 0.01 M KCI solution with a Ag/AgCl reference electrode was used, and an external saturated calomel electrode served as the other reference electrode. The membranes were tested in aqueous solution with varying K + concentration and ionic strength buffered to 0.1 M with NaCl. Interferences were calculated using the Nicolsky equation at 10" 4 and IO "5 M KCI in 0.1 M NaCl. This membrane gave a slope of 58 mV per decade of K + concentration and ion selectivity of log k y = -4.0 as measured using the method described herein. Example 2 Ca 2+ membranes were made according to the method and using the materials as disclosed in Example 1, substituting an equal amount of ETHlOOl (Molecular formula C 3g H 72 N 2 O 8 , molecular weight 685.01, selectophore grade, purchased from Fluka of Buchs, Switzerland) for valinomycin. The resulting membrane gave a slope of 28 mV per decade of Ca + concentration, and an ion selectivity of log ky = -4.0 against Na + . These measurements were made using the technique disclosed in Example 1 , with the exceptions that the internal solution was CaCl 2 , 0.33 M NaCl was used to adjust the ionic strength to 0.1M, and the concentration of CaCl 2 was varied. Example 3 NH 4 + ion-selective membranes were made using the method and the materials disclosed in Example 1 by substituting nonactin (purchased from Sigma located in St. Louis, Missouri) for valinomycin. The slope ofthe membrane was 58 mV per decade of NH 4 + concentration and the interference by Na + had a value of log ky = -2. These measurements were made using the method of Example 1 with the exception that the internal solution was NH 4 C1, NaCl was used to adjust the ionic strength to 0.1M, and the concentration of NH 4 C1 was varied. Example 4 A membrane comprising 29%> PVC (obtained from Fluka of Buchs, Switzerland) 49.1%> EVA (45), 20%) dioctyl adipate (DOA) (selectophore grade from Fluka of Buchs, Switzerland), 1%) valinomycin and 0.1 %> KB (C 6 H 4 C1) 4 (obtained from Fluka of Buchs, Switzerland) by weight was prepared as follows. 3.25 ml of EVA (45) having a concentration of 30 mg/ml was mixed with 1 ml of a valinomycin solution having a concentration of 2 mg/ml. 0.2 ml of KB(C 6 H 4 C1) 4 having a concentration of 1 mg/ml was added to the solution. 39 mg of DO A and 56.5 mg of PVC, previously dissolved in 3 ml of distilled THF, was added to this solution. The solution was cast in a Teflon mold and the membranes were tested as described in the above examples. A membrane electrode slopes of 57 mV per decade of K + concentration and an ion selectivity of log ky = -3.8 against Na + were obtained. All materials and methods were the same as in Example 1 unless otherwise noted. Unless otherwise mentioned, the vendors for particular chemical species listed in the examples are the same as those noted in earlier examples. Example 5 In example 5, as in examples 1-4 which used EVA(45), it is believed that the crystallinity ofthe membranes are below about 3%. %PVC %DOA %EVA %val %KB(Cl? slopefmV. log ky 29 20 50 1 0.1 57 -3.8 19 0 70 1 0.3 49 -3.5 .9 0 68 3 0.3 53 -4.0 0 0 99 1 0 51 -2.2 9 999 1 1 0.1 55 -3.5 99 1 0.2 55 -3.4 99 1 0.3 58 -4.0 98 2 0 42 -3.1 98 2 0.1 57 -3.3 9 988 2 2 0.2 57 -3.6 98 2 0.3 56 -3.4 97 3 0.1 55 -3.0 97 3 0.2 54 -2.1 94 6 0.3 55 -3.1 9 933 6 6 0.6 58 -2.5 Example 6 In example 6, which uses EVA(45), it is believed that the crystallinity ofthe membranes are below about 3%. %PVC %NPOE %EVA %ETHC1001 . %KB(ClPh)., slopefmV) log kyfNa+ 29 20 50 1 0.1 27 -3.7 29 10 60 1 0.2 25 -3.7 29 0 70 1 0.1 18 -3.3 29 0 68 3 0.3 23 -3.4 9 0 88 3 0.3 28 -3.7 0 0 97 3 0.3 28 -4.0 94 6 0.3 29 -3.7 93 6 0.6 29 -4.1 99 1 0.1 28 -3.8 99 1 0.2 28 -3.8 Having thus described certain embodiments ofthe invention, various alterations, modifications and improvements will readily occur to those skilled in the art. Such alterations, modifications, and improvements are intended to be part of this disclosure and are intended to be within the spirit and scope ofthe invention. The materials employed, as well as their shapes and dimensions, may be any required. Accordingly, the foregoing description is by way of example only and is not intended as limiting. The invention is limited only as defined in the following claims and the equivalents thereto. What is claimed is:

Claims: CLAIMS 1. An ion selective membrane, comprising: an ethylene vinyl acetate polymer, said polymer including from about 30 weight percent to about 75 weight percent vinyl acetate monomer and from about 0 percent to about 20 percent by weight plasticizer; and an ion selective material dispersed within the ethylene vinyl acetate polymer, wherein said ion selective membrane is from about 0% crystalline to about 10%> crystalline. 2. An ion selective membrane in accordance with claim 1, wherein said polymer includes from about 35 weight percent to about 55 weight percent vinyl acetate monomer and from about 0 percent to about 5 percent by weight plasticizer. 3. An ion selective membrane in accordance with claim 2, wherein said polymer is substantially devoid of plasticizer. 4. An ion selective membrane in accordance with claims 1, 2 or 3, wherein said polymer includes from about 40 weight percent to about 50 weight percent vinyl acetate monomer. 5. An ion selective membrane in accordance with claims 1, 2 or 3, wherein said polymer includes about 45 weight percent vinyl acetate monomer. 6. An ion selective membrane in accordance with claim 2, wherein said membrane comprises from about 0.1 percent to about 10 percent of said ion selective material by weight. 7. An ion selective membrane in accordance with claim 6, wherein said membrane comprises from about 1 percent to about 6 percent of said ion selective material by weight. 8. An ion selective membrane in accordance with claims 1, 2 or 3, further comprising a salt uniformly incoφorated in said membrane, said salt having a mole percent of said membrane so as to permit increased ion transport within said membrane. 9. An ion selective membrane in accordance with claim 8, wherein said mole percent of said salt is from about 10 percent to about 80 percent of said mole percent of said ion selective material. 10. An ion selective membrane in accordance with claim 8, wherein said mole percent of said salt is from about 33 percent to about 66 percent of said mole percent of said ion selective material. 11. An ion selective membrane in accordance with claims 1, 2 or 3, wherein said membrane has a slope of at least about 50 millivolts per decade of analyte concentration. 12. An ion selective membrane in accordance with claims 1, 2 or 3, wherein said membrane has a slope of at least about 55 millivolts per decade of analyte concentration. 13. An ion selective membrane in accordance with claims 1 , 2 or 3 , wherein said membrane is from about 0%> crystalline to about 5% crystalline. 14. An ion selective membrane in accordance with claims 1, 2 or 3, wherein said membrane is from about 0% crystalline to about 3% crystalline. 15. An ion selective membrane in accordance with claim 1 , further comprising: polyvinyl chloride polymer combined with said ethylene vinyl acetate polymer, wherein said membrane comprises from about 0.1 percent to about 50 percent by weight said polyvinyl chloride polymer. 16. An ion selective membrane, comprising: a polymer formed from the polymerization product of: ethylene monomers; ι vinyl acetate monomers; and monomers of at least one additional vinyl species having a charged site or a polar group, said polymer including from about 30 weight percent to about 55 weight percent vinyl acetate monomer, said polymer being substantially devoid of plasticizer; and an ion selective material dispersed within the polymer, wherein said ion selective membrane is from about 0% crystalline to about 10% crystalline. 17. An ion selective membrane in accordance with claim 16, wherein said charged site or said polar group is selected from the group consisting of carboxyl groups, amino groups, cyano groups, and hydroxyl groups. 18. An ion selective membrane in accordance with claim 16, wherein said polymer comprises at most about 5 percent by weight said monomers of said at least one additional vinyl species. 19. An ion selective membrane in accordance with claim 16, wherein said membrane is from about 0% crystalline to about 5%> crystalline. 20. An ion selective membrane in accordance with claim 16, further comprising: polyvinyl chloride polymer combined with said ethylene vinyl acetate polymer, wherein said membrane comprises from about 0.1 percent to about 50 percent by weight said polyvinyl chloride polymer. 21. An ion selective membrane in accordance with claim 16, wherein said membrane comprises firom about 0.1 percent to about 10 percent of said ion selective material by weight. 22. In an ion selective electrode construction comprising at least one electrode, and an ion selective membrane operatively positioned with respect to said electrode, said membrane comprising: an ethylene vinyl acetate polymer, said polymer having from about 30 weight percent to about 75 weight percent vinyl acetate monomer and from about 0 percent to about 20 percent by weight plasticizer; and an ion selective material dispersed within the ethylene vinyl polymer, wherein said ion selective membrane is from about 0%> crystalline to about 10% crystalline. 23. An ion selective electrode construction in accordance with claim 22, wherein said polymer includes from about 0 weight percent to about 55 weight percent vinyl acetate monomer and about 0 percent to about 5 percent plasticizer by weight plasticizer. 24. An ion selective electrode construction in accordance with claim 23, wherein said polymer is substantially devoid of plasticizer. 25. An ion selective membrane in accordance with claim 22, wherein said membrane comprises from about 0.1 percent to about 10 percent of said ion selective material by weight. 26. An ion selective electrode construction in accordance with claim 22, further comprising: polyvinyl chloride polymer combined with said ethylene vinyl acetate polymer, wherein said membrane comprises from about 0.1 percent to about 50 percent by weight said polyvinyl chloride polymer. 27. A method of forming an ion selective electrode construction for use in ion selective electrode determinations, said method comprising the steps of: providing a contact electrode; and positioning an ion selective membrane in operative position over said contact electrode, said membrane comprising: an ethylene vinyl acetate polymer, said polymer including from about 30 weight percent to about 75 weight percent vinyl acetate monomer and about 0 percent to about 20 percent plasticizer by weight; and an ion selective material dispersed within the ethylene vinyl acetate polymer, wherein said ion selective membrane is from about 0% crystalline to about 10% crystalline. 28. A method in accordance with claim 27, wherein the step of positioning said ion selective membrane includes positioning an ion selective membrane comprising a noncrystalline ethylene vinyl acetate polymer including from about 35 weight percent to about 55 weight percent vinyl acetate and from about 0 percent to about 5 percent plasticizer by weight. 29. A method in accordance with claim 27, wherein the step of positioning said ion selective membrane includes positioning an ion selective membrane comprising a noncrystalline, normal ethylene vinyl acetate polymer including from about 35 weight percent to about 55 weight percent vinyl acetate, from about 0 percent to about 5 percent plasticizer by weight, and from about 0.1 percent to about 50 percent by weight said polyvinyl chloride polymer. 30. A method in accordance with claim 27, wherein the step of positioning an ion selective membrane includes positioning an ion selective membrane including from about 0.1%) to about 10%o ion selective material by weight. 31. A field effect transistor, comprising: a layer of semi-conductor material having a surface; a pair of first diffusion regions of a first doping characteristic at said surface; a second diffusion region of a second doping characteristic at said surface between said pair of first diffusion regions; an electrically insulating material provided on said surface; an ion selective membrane separated from said diffusion regions by said insulating materials; and an electrically insulating encapsulant sealing said transistor from a sample region while leaving exposed a region of said ions select membrane for exposure to a sample, wherein said ion selective membrane includes an ethylene vinyl acetate polymer, said polymer including from about 30 weight percent to about 75 weight percent vinyl acetate monomer and from about 0% to about 20%) by weight plasticizer, and an ion selective material dispersed within the ethylene vinyl acetate polymer, wherein said ion selective membrane is from about 0%> crystalline to about 10% crystalline.

Inventors: HARRISON DANIEL J, NEUFELD AARON, HARRISON, DANIEL, J., NEUFELD, AARON
Applicants: UNIV ALBERTA, THE GOVERNORS OF THE UNIVERSITY OF ALBERTA
Application year: 1996
Main classification: G01N27
IPC codes: G01N27