4-SUBSTITUTED-PHENYL-BORONIC ACIDS AS ENZYME STABILIZERS

Abstract: This invention relates to a liquid composition comprising an enzyme and a phenyl boronic acid derivative enzyme stabilizer of formula (I), wherein R is selected from the group consisting of hydrogen, hydroxy, C1-C6 alkyl, substituted C1-C6 alkyl, C1-C6 alkenyl and substituted C1-C6 alkenyl.

Description: 4-SUBSTITUTED-PHENYL-BORONIC ACIDS AS ENZYME STABILIZERS FIELD OF INVENTION This invention relates to a liquid composition, in particular to a liquid detergent composition, comprising an enzyme and an improved enzyme stabilizer. BACKGROUND OF THE INVENTION Storage stability problems are well known with liquids containing enzyme (s) . Especially in enzyme-containing liquid detergents a major problem, in particular if the detergent contains protease, is that of ensuring enzyme activity over time. The prior art has dealt extensively with improving the storage stability, for example by adding a protease inhibitor. Boric acid and boronic acids are known to reversibly inhibit proteolytic enzymes. A discussion of the inhibition of one serine protease, subtilisin, by boronic acid is provided in Molecular & Cellular Biochemistry 51, 1983, pp. 5-32. Boronic acids have very different capacities as subtilisin inhibitors. Boronic acids containing only alkyl groups such as methyl, butyl or 2-cyclohexylethyl are poor inhibitors with methylboronic acid as the poorest inhibitor, whereas boronic acids bearing aromatic groups such as phenyl, 4-methoxyphenyl or 3,5-dichlorophenyl are good inhibitors with 3, 5-dichlorophenylboronic acid as a particularly effective one (see Keller et al, Biochem. Biophys. Res. Com. 176, 1991, pp. 401-405) . It is also claimed that aryl boronic acids which have a substitution at the 3-position relative to boron are unexpectedly good reversible protease inhibitors. Especially, acetamidophenyl boronic acid is claimed to be a superior inhibitor of proteolytic enzymes (see WO 92/19707) . The inhibition constant (Ki) is ordinarily used as a measure of capacity to inhibit enzyme activity, with a low Ki indicating a more potent inhibitor. However, it has earlier been found that the Ki values of boronic acids do not always tell how effective inhibitors are (see for instance WO 92/19707) . SUMMARY OF THE INVENTION In this invention it is surprisingly found that phenyl boronic acid derivatives substituted in the para- position with a >C=0 adjacent to the phenyl boronic acid have extraordinary good capacities as enzyme stabilizers in liquids. Accordingly, the present invention relates to a liquid composition comprising an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the following formula: wherein R is selected from the group consisting of hydrogen, hydroxy, Ci-Cβ alkyl, substituted Ci-Cβ alkyl, Cι-C 6 alkenyl and substituted Ci-Cβ alkenyl. DETAILED DISCLOSURE OF THE INVENTION One embodiment of the present invention provides a liquid composition comprising an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the following formula: wherein R is selected from the group consisting of hydrogen, hydroxy, Cχ-C 6 alkyl, substituted Cι-C 6 alkyl, Cι-C 6 alkenyl and substituted Ci-Cβ alkenyl. A preferred embodiment of the present invention provides a liquid composition comprising an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the formula disclosed above, wherein R is a Cι-C 6 alkyl, in particular wherein R is CH 3 , CH3CH2 or CH3CH2CH2, or wherein R is hydrogen. A further preferred embodiment of the present invention provides a liquid detergent composition comprising a surfactant, an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the formula disclosed above. Preparation of Phenyl Boronic Acid Derivatives Phenyl boronic acid derivatives may be prepared using methods well known to those skilled in the art, for example by using a Grignard preparation: The Grignard reagent is prepared by the slow dropwise addition of the appropriate bromobenzene starting material in anhydrous ether to magnesium turnings in anhydrous ether. The anhydrous ether may be, e.g., sodium dried diethylether or sodium dried tetrahydrofuran. The reaction is encouraged by the addition of a small iodine crystal. Trimethylborate or tri-n-butylborate in anhydrous ether (e.g. sodium dried diethylether or sodium dried tetrahydrofuran) is cooled to about -70°C and the Grignard reagent is added dropwise over a period of approximately 2 hours while keeping the borate solution at about -70°C and continuously agitating. The reaction mixture is allowed to warm to room temperature overnight whereupon it is hydrolysed by the dropwise addition of cold dilute sulphuric acid. The ether layer is separated and the aqueous layer extracted with ether. The ether containing fractions are combined and the solvent removed. The residue is made distinctly alkaline and any methanol or butanol so formed is removed. The alkaline solution is made acidic and cooled and the resulting crystals of desired boronic acid are removed by filtration. All products are preferably recrystallized from distilled water or some other appropriate solvent. Preparation of, e.g., 4-formyl-phenyl-boronic acid, using the method disclosed above, has been described in Chem. Ber. 123, 1990, pp. 1841-1843. The phenyl boronic acids may also be prepared using either direct lithiation of the benzene and/or lithiation of the bromide. Any nuclear substitution or protection of functional groups may be achieved by using standard methods well known to those skilled in the art. Stabilizers According to the invention the liquid composition may contain up to 500 mM of the stabilizer (the phenyl boronic acid derivative) , preferably the detergent composition may contain 0.001-250 mM of the stabilizer, more preferably the liquid composition may contain 0.005-100 mM of the stabilizer, most preferably the liquid composition may contain 0.01-10 M of the stabilizer. The phenyl boronic acid derivative may be an acid or the alkali metal salt of said acid. Enzymes According to the invention the liquid composition contains at least one enzyme. The enzyme may be any commercially available enzyme, in particular an enzyme selected from the group consisting of proteases, amylases, upases, cellulases, oxidoreductases and any mixture thereof. Mixtures of enzymes from the same class (e.g. proteases) are also included. According to the invention a liquid composition comprising a protease is preferred; more preferred is a liquid composition comprising two or more enzymes in which the first enzyme is a protease and the second enzyme is selected from the group consisting of amylases, upases, cellulases and oxidoreductases; even more preferred is a liquid composition in which the first enzyme is a protease and the second enzyme is a lipase. The amount of enzyme used in the liquid composition varies according to the type of enzyme (s) . The amount of each enzyme will typically be 0.04-40 μM, in particular 0.2-30 μM, especially 0.4-20 μM (generally 1-1000 mg/1, in particular 5- 750 mg/1, especially 10-500 mg/1) calculated as pure enzyme protein. Proteases : Suitable proteases include those of animal, vegetable or microbial origin. Microbial origin is preferred. Chemically or genetically modified mutants are included. The protease may be a serine protease, preferably an alkaline mi¬ crobial protease or a trypsin-like protease. Examples of al¬ kaline proteases are subtilisins, especially those derived from Bacillus, e.g., subtilisin Novo, subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin 168 (described in WO 89/06279) . Examples of trypsin-like proteases are tryp- sin (e.g. of porcine or bovine origin) and the Fusarium pro¬ tease described in WO 89/06270. Preferred commercially available protease enzymes in¬ clude those sold under the tradenames Alcalase, Savinase, Primase, Durazym, and Esperase by Novo Nordisk A/S (Denmark) , those sold under the tradename Maxatase, Maxacal, Maxapem and Properase by Gist-Brocades, those sold under the tradename Purafect and Purafect OXP by Genencor International, and those sold under the tradename Opticlean and Optimase by Sol- vay Enzymes. Lipases : Suitable upases include those of bacterial or fungal origin. Chemically or genetically modified mutants are included. Examples of useful lipases include a Humicola lanugi- nosa lipase, e.g., as described in EP 258 068 and EP 305 216, a Rhizomucor miehei lipase, e.g., as described in EP 238 023, a Candida lipase, such as a C. antarctica lipase, e.g., the C. antarctica lipase A or B described in EP 214 761, a Pseu- domonas lipase such as a P. pseudoalcaligenes and P. alcali- genes lipase, e.g., as described in EP 218 272, a P. cepacia lipase, e.g., as described in EP 331 376, a P. stutzeri li¬ pase, e.g., as disclosed in BP 1,372,034, a P. fluorescens lipase, a Bacillus lipase, e.g., a B. subtilis lipase (Dartois et al. , (1993), Biochemica et Biophysica acta 1131, 253-260), a B. stearothermophilus lipase (JP 64/744992) and a B. pumilus lipase (WO 91/16422) . Furthermore, a number of cloned lipases may be useful, including the Penicillium camenbertii lipase described by Ya- maguchi et al. , (1991), Gene 103, 61-67), the Geotricum can- didum lipase (Schimada, Y. et al. , (1989), J. Biochem. 106, 383-388), and various Rhizopus lipases such as a R. delemar lipase (Hass, M.J et al. , (1991), Gene 109, 117-113), a R. niveus lipase (Kugimiya et al. , (1992), Biosci . Biotech. Bio¬ chem. 56, 716-719) and a R. oryzae lipase. Other types of lipolytic enzymes such as cutinases may also be useful, e.g., a cutinase derived from Pseudomonas mendocina as described in WO 88/09367, or a cutinase derived from Fusarium solani pisi (e.g. described in WO 90/09446) . Especially suitable lipases are lipases such as Ml Li¬ pase™, Luma fast™ and Lipomax™ (Genencor) , Lipolase™ and Lipolase Ultra™ (Novo Nordisk A/S) , and Lipase P "A ano" (Amano Pharmaceutical Co. Ltd.) . Amylases: Suitable amylases (a and/or β) include those of bacterial or fungal origin. Chemically or genetically mod¬ ified mutants are included. Amylases include, for example, a- amylases obtained from a special strain of B. licheniformis, described in more detail in British Patent Specification No. 1,296,839. Commercially available amylases are Duramyl™, Ter- mamyl™, Fungamyl™ and BAN™ (available from Novo Nordisk A/S) and Rapidase™ and Maxamyl P™ (available from Gist-Brocades) . Cellulases: Suitable cellulases include those of bacte- rial or fungal origin. Chemically or genetically modified mu¬ tants are included. Suitable cellulases are disclosed in US 4,435,307, which discloses fungal cellulases produced from Humicola insolens. Especially suitable cellulases are the cellulases having color care benefits. Examples of such cel- lulases are cellulases described in European patent applica¬ tion No. 0 495 257. Commercially available cellulases is Celluzyme™ pro¬ duced by a strain of Humicola insolens, (Novo Nordisk A/S) , and KAC-500(B)™ (Kao Corporation) . Oxidoreductases: Any oxidoreductase suitable for use in a liquid composition, e.g., peroxidases or oxidases such as laccases, can be used herein. Suitable peroxidases herein include those of plant, bacterial or fungal origin. Chemically or genetically modified mutants are included. Examples of suitable peroxidases are those derived from a strain of Coprinus, e.g., C. cinerius or C. macrorhizus, or from a strain of Bacillus, e.g., B. pumilus, particularly peroxidase according to WO 91/05858. Suitable laccases herein include those of bacterial or fungal origin. Chemically or genetically modified mutants are included. Examples of suitable laccases are those obtainable from a strain of Trametes, e.g., T. villosa or T. versicolor, or from a strain of Coprinus, e.g., C. cinereus, or from a strain of Myceliophthora, e.g., M. thermophila. Detergents According to the invention the liquid detergent composition will beside enzyme (s) and stabilizer comprise a surfactant. The detergent composition may, e.g., be a laundry detergent composition or a dishwashing detergent composition. The detergent may be aqueous, typically containing up to 70 % water and 0-30 % organic solvent, or nonaqueous. The detergent composition comprises one or more surfactants, each of which may be anionic, nonionic, cationic, or amphoteric (zwitterionic) . The detergent will usually contain 0-50% of anionic surfactant such as linear alkylben- zenesulfonate (LAS), alpha-olefinsulfonate (AOS) , alkyl sulfate (fatty alcohol sulfate) (AS) , alcohol ethoxysulfate (AEOS or AES) , secondary alkanesulfonates (SAS) , alpha-sulfo fatty acid methyl esters, alkyl- or alkenylsuccinic acid, or soap. It may also contain 0-40% of nonionic surfactant such as alcohol ethoxylate (AEO or AE) , alcohol propoxylate, carboxylated alcohol ethoxylates, nonylphenol ethoxylate, alkylpolyglycoside, alkyldimethylamine oxide, ethoxylated fatty acid monoethanolamide, fatty acid monoethanolamide, or polyhydroxy alkyl fatty acid amide (e.g. as described in WO 92/06154) . Normally the detergent contains 1-65% of a detergent builder, but some dishwashing detergents may contain even up to 90% of a detergent builder, or complexing agent such as zeolite, diphosphate, triphosphate, phosphonate, citrate, nitrilotriacetic acid (NTA) , ethylenediaminetetraacetic acid (EDTA) , diethylenetriaminepentaacetic acid (DTMPA) , alkyl- or alkenylsuccinic acid, soluble silicates or layered silicates (e.g. SKS-6 from Hoechst) . The detergent builders may be subdivided into phosphorus-containing and non-phosphorous-containing types. Examples of phosphorus-containing inorganic alkaline detergent builders include the water-soluble salts, especially alkali metal pyrophosphates, orthophosphates, polyphosphates and phosphonates. Examples of non-phosphorus-containing inorganic builders include water-soluble alkali metal carbonates, borates and silicates as well as layered disilicates and the various types of water-insoluble crystalline or amorphous alumino silicates of which zeolites is the best known representative. Examples of suitable organic builders include alkali metal, ammonium or substituted ammonium salts of succinates, malonates, fatty acid malonates, fatty acid sulphonates, carboxymethoxy succinates, polyacetates, carboxylates, polycarboxylates, aminopolycarboxylates and polyacetyl carboxylates. The detergent may also be unbuilt, i.e. essentially free of detergent builder. The detergent may comprise one or more polymers. Examples are carboxymethylcellulose (CMC), poly(vinyl- pyrrolidone) (PVP) , polyethyleneglycol (PEG), poly(vinyl alcohol) (PVA) , polycarboxylates such as polyacrylates, polymaleates, maleic/acrylic acid copolymers and lauryl methacrylate/acrylic acid copolymers. The detergent composition may contain bleaching agents of the chlorine/bromine-type or the oxygen-type. The bleaching agents may be coated or encapsulated. Examples of inorganic chlorine/bromine-type bleaches are lithium, sodium or calcium hypochlorite or hypobromite as well as chlorinated trisodium phosphate. The bleaching system may also comprise a H 2 0 2 source such as perborate or percarbonate which may be combined with a peracid-forming bleach activator such as tetraacetylethylenediamine (TAED) or nonanoyloxybenzene- sulfonate (NOBS) . Examples of organic chlorine/bromine-type bleaches are heterocyclic N-bromo and N-chloro imides such as trichloroisocyanuric, tribromoisocyanuric, dibromoisocyanuric and dichloroisocyanuric acids, and salts thereof with water solubilizing cations such as potassium and sodium. Hydantoin compounds are also suitable. The bleaching system may also comprise peroxyacids of, e.g., the amide, imide, or sulfone type. In dishwashing detergents the oxygen bleaches are preferred, for example in the form of an inorganic persalt, preferably with a bleach precursor or as a peroxy acid com¬ pound. Typical examples of suitable peroxy bleach compounds are alkali metal perborates, both tetrahydrates and monohydrates, alkali metal percarbonates, persilicates and perphosphates. Preferred activator materials are TAED or NOBS. The enzyme (s) of the detergent composition of the invention may additionally be stabilized using conventional stabilizing agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol, or lactic acid. The detergent may also contain other conventional detergent ingredients such as, e.g., fabric conditioners in¬ cluding clays, deflocculant material, foam boosters/foam depressors (in dishwashing detergents foam depressors) , suds suppressors, anti-corrosion agents, soil-suspending agents, anti-soil-redeposition agents, dyes, dehydrating agents, bactericides, optical brighteners, or perfume. The pH (measured in aqueous solution at use con¬ centration) will usually be neutral or alkaline, e.g. in the range of 7-11. Particular forms of laundry detergent compositions within the scope of the invention include: 1) An aqueous liquid detergent composition comprising Linear alkylbenzenesulfonate (cal¬ 15 - 21% culated as acid) Alcohol ethoxylate (e.g. C 1 2- 15 alco¬ hol, 7 EO or C12-15 alcohol, 5 EO) 12 - 18% Soap as fatty acid (e.g. oleic acid) 3 - 13% Alkenylsuccinic acid (C12-14) 0 - 13% Aminoethanol 8 - 18% Citric acid 2 - 8% Phosphonate 0 3% Polymers (e.g. PVP, PEG) 0 3% Borate (as B4O7) 0 2% Ethanol 0 3% Propylene glycol 8 14% Enzymes (calculated as pure enzyme 0.0001 - 0.1% protein) Minor ingredients (e.g. dispersants, suds suppressors, perfume, optical 0 5% brightener) 2) An aqueous structured liquid detergent composition compris¬ ing Linear alkylbenzenesulfonate (calculated as acid) 15 - 21% Alcohol ethoxylate (e.g. C12-15 alcohol, 7 EO, 3 - 9% or C12-15 alcohol, 5 EO) Soap as fatty acid (e.g. oleic 3 - 10% acid) Zeolite (as NaAlSi0 4 ) 14 - 22% Potassium citrate 9 - 18% Borate (as B 4 O7) 0 - 2% Carboxymethylcellulose 0 - 2% Polymers (e.g. PEG, PVP) 0 - 3% Anchoring polymers such as, e.g., lauryl methacrylate/acrylic acid 0 - 3% copolymer; molar ratio 25:1; MW 3800 Glycerol 0 - 5% Enzymes (calculated as pure enzyme 0.0001 - 0.1% protein) Minor ingredients (e.g. dispersants, suds suppressors, per¬ 0 - 5% fume, optical brighteners) 53) An aqueous liquid detergent composition comprising Linear alkylbenzenesulfonate (calculated as acid) 15 - 23% Alcohol ethoxysulfate (e.g. C12-15 alcohol, 2-3 EO) 8 - 15% Alcohol ethoxylate (e.g. C12-15 al- cohol, 7 EO, 3 - 9% or C 12 - 15 alcohol, 5 EO) Soap as fatty acid (e.g. lauric 0 - 3% acid) Aminoethanol 1 - 5% Sodium citrate 5 - 10% Hydrotrope (e.g. sodium 2 - 6% toluensulfonate) Borate (as B 4 0 7 ) 0 - 2% Carboxymethylcellulose 0 - 1% Ethanol 1 - 3% Propylene glycol 2 - 5% Enzymes (calculated as pure enzyme 0.0001 - 0.1% protein) Minor ingredients (e.g. polymers, dispersants, perfume, optical 0 - 5% brighteners) 4) An aqueous liquid detergent composition comprising Linear alkylbenzenesulfonate (calculated as acid) 20 - 32% Alcohol ethoxylate (e.g. C1 2 - 15 alco¬ hol, 7 EO, 6 - 12% or C 12 - 15 alcohol, 5 EO) Aminoethanol 2 - 6% Citric acid 8 - 14% Borate (as B4O 7 ) 1 - 3% Polymer (e.g. maleic/acrylic acid copolymer, anchoring polymer such as, e.g., lauryl 0 - 3% methacrylate/acrylic acid copolymer) Glycerol 3 - 8% Enzymes (calculated as pure enzyme 0.0001 - - 0.1% protein) Minor ingredients (e.g. hydrotropes, dispersants, perfume, optical - 5^ brighteners) 5) Detergent formulations as described in 1) - 4) wherein all or part of the linear alkylbenzenesulfonate is replaced by (Ci 2 -Cχ 8 ) alkyl sulfate. 5 6) Detergent formulations as described in 1) - 5) which contain a stabilized or encapsulated peracid, either as an additional component or as a substitute for already specified bleach systems. 107) Detergent composition formulated as a nonaqueous detergent liquid comprising a liquid nonionic surfactant such as, e.g., linear alkoxylated primary alcohol, a builder system (e.g. phosphate), enzyme and alkali. The detergent may also comprise anionic surfactant and/or a bleach system. 15 Particular forms of dishwashing detergent composi¬ tions within the scope of the invention include: 1) LIQUID DISHWASHING COMPOSITION WITH CLEANING SURFACTANT SYSTEM 20 Nonionic surfactant 0 - 1.5% Octadecyl dimethylamine N-oxide dihydrate 0 - 5% 80:20 wt.C18/C16 blend of octadecyl dimethylamine N-oxide dihydrate and hexadecyldimethyl amine N-oxide 0 - 4% dihydrate 70:30 wt.C18/C16 blend of octadecyl bis (hydroxyethyl) amine N-oxide anhydrous and hexadecyl bis 0 - 5% (hydroxyethyl) amine N-oxide anhydrous C 13 -C 15 alkyl ethoxysulfate with an average degree of ethoxylation of 3 0 - 10% Ci 2 -Cχ 5 alkyl ethoxysulfate with an average degree of ethoxylation of 3 0 - 5% 14 13 -C 15 ethoxylated alcohol with an average degree of ethoxylation of 12 0 - 5% A blend of C 12 -C 15 ethoxylated alco¬ hols with an average degree of 0 - 6.5% ethoxylation of 9 A blend of C 13 -C 15 ethoxylated alco¬ hols with an average degree of 0 - 4% ethoxylation of 30 Sodium disilicate 0 - 33% Sodium tripolyphosphate 0 - 46% Sodium citrate 0 - 28% Citric acid 0 - 29% Sodium carbonate 0 - 20% ' Sodium perborate monohydrate 0 - 11.5% Tetraacetylethylenediamine (TAED) 0 - 4% Maleic acid/acrylic acid copolymer 0 - 7.5% Sodium sulphate 0 - 12.5% Enzymes 0.0001 - 0.1% 2) NON-AQUEOUS LIQUID AUTOMATIC DISHWASHING COMPOSITION Liquid nonionic surfactant (e.g. alcohol ethoxylates) 2.0 - 10.0% Alkali metal silicate 3.0 - 15.0% Alkali metal phosphate 20.0 - 40.0% Liquid carrier selected from higher glycols, polyglycols, polyoxides, 25.0 - 45.0% glycolethers Stabilizer (e.g. a partial ester of phosphoric acid and a Cι 6 -Cι 8 alkanol) 0.5 - 7.0% Foam suppressor (e.g. silicone) 0 - 1.5% Enzymes 0.0001 - 0.1% 53) NON-AQUEOUS LIQUID DISHWASHING COMPOSITION Liquid nonionic surfactant (e.g. alcohol ethoxylates) 2.0 - 10.0% Sodium silicate 3.0 - 15.0% Alkali metal carbonate 7.0 - 20.0% Sodium citrate 0.0 - 1.5% Stabilizing system (e.g. mixtures of finely divided silicone and low molecular weight dialkyl polyglycol 0.5 - 7.0% ethers) Low molecule weight polyacrylate polymer 5.0 - 15.0% Clay gel thickener (e.g. bentonite) 0.0 - 10.0% Hydroxypropyl cellulose polymer 0.0 - 0.6% Enzymes 0.0001 - 0.1% Liquid carrier selected from higher lycols, polyglycols, polyoxides and Balance glycol ethers ) ' THIXOTROPIC LIQUID AUTOMATIC DISHWASHING COMPOSITION C 12 -C 1 fatty acid 0 - 0.5% Block co-polymer surfactant 1.5 - 15.0% Sodium citrate 0 - 12% " Sodium tripolyphosphate 0 - 15% Sodium carbonate 0 - 8% Aluminium tristearate 0 - 0.1% Sodium cumene sulphonate 0 - 1.7% Polyacrylate thickener 1.32 - 2.5% Sodium polyacrylate 2.4 - 6.0% Boric acid 0 - 4.0% Sodium formate 0 - 0.45% Calcium formate 0 - 0.2% Sodium n-decydiphenyl oxide disulphonate 0 - 4.0% Monoethanol amine (MEA) 0 - 1.86% Sodium hydroxide (50%) 1.9 - 9.3% 1,2-Propanediol 0 - 9.4% Enzymes 0.0001 - 0.1% Suds suppressor, dye, perfumes, water Balance 5) LIQUID AUTOMATIC DISHWASHING COMPOSITION Alcohol ethoxylate 0 - 20% Fatty acid ester sulphonate 0 - 30% Sodium dodecyl sulphate 0 - 20% Alkyl polyglycoside 0 - 21% Oleic acid 0 - 10% Sodium disilicate monohydrate 18 - 33% Sodium citrate dihydrate 18 - 33% Sodium stearate 0 — __ . ) ~6 Sodium perborate monohydrate 0 - 13% ' Tetraacetylethylenediamine (TAED) 0 - 8% Maleic acid/acrylic acid copolymer 4 - 8% Enzymes 0.0001 - 0.1% 56) LIQUID AUTOMATIC DISHWASHING COMPOSITION CONTAINING PROTECTED BLEACH PARTICLES • Sodium silicate 5 - 10% Tetrapotassium pyrophosphate 15 - 25% Sodium triphosphate 0 - 2% Potassium carbonate 4 - 8% Protected bleach particles, e.g. chlorine 5 - 10% Polymeric thickener 0.7 - 1.5% Potassium hydroxide 0 - 2% Enzymes 0.0001 - 0.1% Water Balance 7) Automatic dishwashing compositions as described in 1) and 105), wherein perborate is replaced by percarbonate. 8) Automatic dishwashing compositions as described in 1) , which additionally contain a manganese catalyst. The manganese catalyst may, e.g., be one of the compounds described in "Efficient manganese catalysts for low-temperature bleaching", Nature 369, 1994, pp. 637-639. Tests of Stabilizers According to the invention the effectiveness of each stabilizer may be tested in one or more of the following tests : a) Storage Stability Test in Liquid Detergent: Enzyme (s) and stabilizer are added to a liquid detergent formulation and stored at well defined conditions. The enzyme activity of each enzyme is determined as a function of time, e.g. after 0, 3, 7 and 14 days. To calculate the inhibition efficiency from the storage stability date a reaction mechanism is proposed. The following reactions give a relatively simple, but yet plaus¬ ible, mechanism for a liquid detergent containing protease (P) , lipase (L) , and inhibitor (I) : I) Autodigestion of protease: II) Denaturation of protease: P → Dp III) Inhibition of protease: P + I <-» PI IV) Protease digestion of inhibited enzyme: V) Denaturation of inhibited enzyme: VI) Protease digestion of lipase: P + L → P + D L VII) Denaturation of lipase: L → D L where D P and D L are denatured (i.e. non-active) protease and lipase. From these reactions three coupled differential equations are derived describing the deactivation of P, L and PI. The reaction rate constants are derived from storage stability data by the use of a parameter estimation method (Gauss-Newton with the Levenberg modification) . The storage stability data give the concentration of (P+PI) and L as a function of time. Reaction III is much faster than the other reactions and equilibrium is assumed in the calculations . Reaction IV is excluded from the system to reduce the number of parameters thereby describing the stability of the inhibited enzyme by only one reaction rate constant (from equation V) . In all experiments there is a large surplus of inhibitor molecules compared to protease molecules, i.e. a constant concentration of inhibitor (corresponding to the added amount of inhibitor) is a reasonable assumption. The specific values of the reaction rate constants are somewhat sensitive to small variations in the data, but the sensitivity is reduced significantly by giving the results relatively to the value from Boric Acid. An improvement factor is thus derived: Kj (Boric Acid) IF ! = Ki(Inhibitor) IFi measures the inhibition efficiency given by the inhibition constants Ki from reaction III. b) Determination of Kj: The inhibition constant Ki may be determined by using standard methods, for reference see Keller et al, Biochem. Biophys. Res. Com. 176, 1991, pp.401-405; J. Bieth in Bayer-Symposium "Proteinase Inhibitors", pp. 463-469, Springer-Verlag, 1974 and Lone Kierstein Hansen in "Deter¬ mination of Specific Activities of Selected Detergent Pro¬ teases using Protease Activity, Molecular Weights, Kinetic Parameters and Inhibition Kinetics", PhD-report, Novo Nordisk A/S and University of Copenhagen, 1991. The invention is further illustrated in the following examples which are not intended to be in any way limiting to the scope of the invention as claimed. EXAMPLE 1 Preparation of 4-Formyl-Phenyl-Boronic Acid 4-Formyl-phenyl-boronic acid may be prepared as disclosed in Chem. Ber. 123, 1990, pp. 1841-1843, or it may be bought at Lancaster Synthesis GmbH (4-Formylbenzeneboronic acid) . EXAMPLE 2 Determination of Kj The inhibition constant K x for the inhibition of Savinase™ (available from Novo Nordisk A/S) was determined using standard methods under the following conditions: Substrate: Succinyl-Alanine-Alanine-Proline-Phenylalanine- para-nitro-anilide = SAAPFpNA (Sigma S-7388) . Buffer: 0.1 M Tris-HCl pH 8.6; 25°C. Enzyme concentration in assay: Savinase: 1 X 10 "10 - 3 x 10 "10 M The initial rate of substrate hydrolysis was deter- mined at nine substrate concentrations in the range of 0.01 to 2 mM using a Cobas Fara automated spectrophotometer. The kinetic parameters V ma χ and K m were determined using ENZFITTER (a non-linear regression data analysis program) . k C at was calculated from the equation V max = k cat X [E 0 ] . The concentration of active enzyme [E 0 ] was determined by active site titration using tight-binding protein proteinase inhibitors. The inhibition constant K was calculated from plots of K m /k ca as a function of the concentration of inhibitor. The inhibitors were assumed to be 100% pure and the molar concentrations were determined using weighing numbers and molecular weights. The results of the inhibition constants K of the phenyl boronic acid derivative enzyme stabilizers tested are listed below: Inhibitor: Ki (Savinase) : Boric acid 20 mM 4-formyl-phenyl-boronic acid 0.3 mM For comparison reasons acetamidophenyl boronic acid was also tested in the same system giving the following results: Inhibitor: K (Savinase) : Boric acid 20 mM acetamidophenyl boronic acid 1 mM It appears from the results given above that the inhibiting properties of 4-formyl-phenyl boronic acid is at least three times better than those of acetamidophenyl boronic acid. EXAMPLE 3 Storage Stability Test in Liquid Detergent Phenyl boronic acid derivatives were also tested in storage stability tests in liquid detergents using the method described previously under the following conditions: Detergent base (US-type) % wt (as pure components) Nansa 1169/p 10.3 (Linear Alkylbenzene Sulfonate,LAS) Berol 452 3.5 (Alkyl Ether Sulfate, AES) Oleic acid 0.5 Coconut fatty acid 0.5 Dobanol 25-7 6.4 (Alcohol Ethoxylate, AEO) Sodium xylene sulfonate 5.1 Ethanol 0.7 MPG 2.7 (Mono Propylene Glycol) Glycerol 0.5 Sodium sulfate 0.4 Sodium carbonate 2.7 Sodium citrate 4.4 Citric acid 1.5 Water 60.8 Enzyme dosage: 1% w/w Savinase (14 KNPU/g) Enzyme Stabilizer Dosage: 5 mmole/kg (for boric acid 160 mmole/kg) Storage: 0, 3, 7 and 14 days at 30°C The results of the inhibition effectiveness IFi of the phenyl boronic acid enzyme stabilizers tested are listed below: Inhibitor: Improvement Factor IF! Boric acid 1 4-formyl-phenyl-boronic acid 1000 For comparison reasons acetamidophenyl boronic acid, 2-formyl- phenyl-boronic acid and 3-formyl-phenyl-boronic acid (all bought at Lancaster) were tested in the same system giving the following results: Inhibitor: Improvement Factor IFi Boric acid 1 acetamidophenyl boronic acid 300 2-formyl-phenyl-boronic acid 36 3-formyl-phenyl-boronic acid 230 It appears from the results given above that the storage stability properties of 4-formyl-phenyl boronic acid is at least three times better than those of acetamidophenyl boronic acid, and at least four times better than those of 3-formyl- phenyl-boronic acid, and at least 25 times better than those of 2-formyl-phenyl-boronic acid (all calculated on molar basis) . EXAMPLE 4 Storage Stability Test in a Commercial Detergent The inhibition effectiveness IFi of 4-formyl-phenyl- boronic acid was also found in a commercial detergent Omo Micro. Omo Micro was bought in a Danish supermarket. The enzymes were inactivated at 90°C (overnight) . The following dosages in the detergent were used: 4-Formyl-phenyl-boronic acid: 1.33 mM, or Boric acid: 160 mM, and Protease: 1% w/w Savinase (8 KNPU/g) , and Lipase: 1% w/w Lipolase (100 KLU/g) . Storage: 0, 7, 15, and 21 days at 40°C. Result: IFi = 2500. EXAMPLE 5 Storage Stability Test of 4-Carboxybenzeneboronic Acid in Liquid Detergent 4-Carboxybenzeneboronic acid (bought at Lancaster) was tested in a storage stability test in a liquid detergent using the method described previously under the following conditions: Detergent base (US-type) % wt (as pure components) Nansa 1169/p 10.3 (Linear Alkylbenzene Sulfonate,LAS) Berol 452 3.5 (Alkyl Ether Sulfate, AES) Oleic acid 0.5 Coconut fatty acid 0.5 Dobanol 25-7 6.4 (Alcohol Ethoxylate, AEO) Sodium xylene sulfonate 5.1 Ethanol 0.7 MPG 2.7 (Mono Propylene Glycol) Glycerol 0.5 Sodium sulfate 0.4 Sodium carbonate 2.7 Sodium citrate 4.4 Citric acid 1.5 Water 60.8 Enzyme dosage: 1% w/w Savinase (14 KNPU/g) Enzyme Stabilizer Dosage: 5 mmole/kg (for boric acid 160 mmole/kg) Storage: 0, 2, 7 and 14 days at 30°C Result: IFi = 22.

Claims: CLAIMS 1. A liquid composition comprising an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the following 5 formula: 10 where R is selected from the group consisting of hydrogen, hydroxy, Cι-C 6 alkyl substituted Cι-C 6 alkyl, Cι~C 6 alkenyl and substituted Ci-Cβ alkenyl. 15 2. A liquid composition according to claim 1, wherein R is Cχ~ ζ alkyl. 3. A liquid composition according to claim 1, wherein R is 20 hydrogen. 4. A liquid composition according to any of claims 1-3, wherein the enzyme is a protease. 255. A liquid composition according to claim 1, additionally comprising a second enzyme, in particular an amylase, a lipase, a cellulase or an oxidoreductase, or any mixture thereof. 306. A liquid composition according to claim 5, wherein the second enzyme is a lipase. 7. A liquid composition according to any of claims 1-6, wherein said phenyl boronic acid derivative enzyme stabilizer is the alkali metal salt of the boronic acid. 58. A liquid composition according to any of claims 1-7, wherein said phenyl boronic acid derivative enzyme stabilizer is added in an amount of up to 500 mM, preferably in an amount of 0.001-250 mM, more preferably in an amount of 0.005-100 mM, most preferably in an amount of 0.01-10 mM. 10 9. A liquid detergent composition comprising a surfactant, an enzyme and a phenyl boronic acid derivative enzyme stabilizer of the following formula: 15 20 where R is selected from the group consisting of hydrogen, hydroxy, Ci-Cβ alkyl, substituted Cι-C 6 alkyl, Cι~C 6 alkenyl and substituted Cι-C 6 alkenyl. 10. A liquid detergent composition according to claim 9, 25 wherein R is Ci-Cβ alkyl. 11. A liquid detergent composition according to claim 9, wherein R is hydrogen. 3012. A liquid detergent composition according to any of claims 9-11, wherein the enzyme is a protease. 13. A liquid detergent composition according to claim 9, additionally comprising a second detergent-compatible enzyme, in particular an amylase, a lipase, a cellulase or an oxidoreductase, or any mixture thereof. 14. A liquid detergent composition according to claim 13, 5 wherein the second enzyme is a lipase. 15. A liquid detergent composition according to any of claims 9-14, wherein said phenyl boronic acid derivative enzyme stabilizer is the alkali metal salt of the boronic acid. 10 16. A liquid detergent composition according to any of claims 9-15, wherein said phenyl boronic acid derivative enzyme stabilizer is added in an amount of up to 500 mM, preferably in an amount of 0.001-250 mM, more preferably in an amount of 150.005-100 mM, most preferably in an amount of 0.01-10 mM.

Inventors: DEANE-WRAY ALLISON, NIELSEN LONE KIERSTEIN, DEANE-WRAY, ALLISON, NIELSEN, LONE, KIERSTEIN
Applicants: DEANE WRAY ALLISON, NIELSEN LONE KIERSTEIN, NOVONORDISK AS, DEANE-WRAY, ALLISON, NIELSEN, LONE, KIERSTEIN, NOVO NORDISK A/S
Application year: 1996
Main classification: C11D3
IPC codes: C11D3, C11D17, C12N9