AGENTS FOR REDUCING WEIGHT

Abstract: Use of amine and amidine containing compounds of formula (I) such as galegine and related substituted amine and amidine containing compounds as agents for reducing weight in mammals, pharmaceutical formulations containing compound of formula (I) and methods of attaining weight reduction in mammals.

Description: Agents for Reducing Weight Field of the Invention The present invention relates to the use of amine and amidine containing compounds such as galegine ( (3-methyl-2- butenyl) guanidine) and related substituted amine and amidine containing compounds, as agents for reducing weight in mammals. Background of the Invention Being obese or over-weight is a common problem. Aggravating factors may be depression or other psychosocial problems. Patients receiving drug treatment are also often predisposed to weight gain. Obesity can be controlled to some extent by strict dieting, however, diering is seldom successful in the long term as the subject may find it difficult to stick to a dieting regime. Obesity can aggravate certain medical conditions, and is a risk factor for heart disease. Galegine is an example of an amidine group containing compound which is known to have hypoglycaemic effect. It has now been unexpectedly found that when galegine is administered to a mammal, a weight reduction effect is observed. Summary of the Invention One aspect of the present invention provides the use of a compound of formula (I) : n wherein R 1 R is selected from the group phenyl and C = CR 3 - where R* R 1 , R 2 and R 3 are independently selected from the group H, C w alkyl, C 2 ^ alkenyl, and C^ alkynyl; R 4 and R 5 are independently selected from H, C,. 6 alkyl, C-.^ alkenyl, and C^ alkynyl; NH R' is selected from the group H or C - R* wherein R* is selected from the group H, NH 2 and C w alkyl; n is a whole integer from 0 to 6; and physiologically acceptable salts thereof in the manufacture of a composition for weight reduction. Preferably R 1 , R 2 , R 3 , R* and R s are independently selected from the group H, C,^ alkyl, C ω alkenyl and C 2 . 3 alkynyl; NH R' is selected from the group H or C - R β wherein R" is selected from the group NH,, H and C,^ alkyl; and n is a whole integer from 0 to 2; and physiologically acceptable salts thereof. In a preferment, the invention provides the use of a compound of formula (II) in the manufacture of a composition for weight reduction, wherein R 1 , R 2 , R 3 , R 4 and R 5 independently selected from hydrogen, C,^ alkyl, C,^ alkenyl, C,. 6 alkynyl and NH,; and n is from 0 to 6; and physiologically acceptable salts thereof. Compounds of formula (I) or formula (II) hereafter are referred to as "weight reducing agent" . Another aspect of the present invention provides a method of reducing weight in a subject which comprises administering to a subject an effective non-toxic amount of a weight reducing agent of formula (I) . In a preferment, the effective non-toxic amount of weight reducing agent is of formula (II) . A further aspect of the present invention is the suppression of appetite i.e. food intake in a subject genetically predisposed to obesity. Thus it has been found that food intake is suppressed in genetically obese subjects, cut does not show a longtem suppression in a normal subject. The weight reduction effect demonstrated by weight reducing agents of the present invention is unexpected since reduction in • glucose leveis caused by known hypoglycaemic compounds does not necessarily lead to weight reduction and may in fact lead to increase in weight. Preferably, R 1 and R 2 of formula (II) are both C,^ alkyl, or C 2 „ 5 alkenyl or C-_s alkynyl, particularly methyl or ethyl; ethenyl or rαethenyl; methynyl or ethynyl; or mixtures thereof. Generally, R 3 of formula (II) is hydrogen or C,^ alkyl. R 4 and R 5 of formula (II) are preferably hydrogen or C,^ alkyl; and n is usually 1 or 2. A particularly preferred compound is galegine, (3-methyl-2- butenyl) guanidine, where R 1 and R 2 are both methyl, R 3 , R 4 and R 5 are hydrogen, and n is unity. Also included as ^n aspect of the invention are novel compounds according to formula (I) and/or (II) such as γ,γ- dimethylallyl amidine and physiologically acceptable salts thereof, such as acid addition salts and the like. Galegine can be isolated from the plant Galeσa officinalis (e.g. Tanret, omot Rend 158 1182, (1914) ; Markovic & Dittertova Che Zvesti 9 576 (1955)). Extracts from the plant Galeσa officinalis can be extracted using solvent systems comprising polar organic solvent systems or solvent systems comprising a mixture of at least one polar organic solvent in admixture with water. Suitable polar organic solvents include supercritical CO,, alcohols such as -ethanol and ethanol, and physiologically acceptable giycols sucn as propylene glycol. It is preferred if the polar organic solvent is one which is pharmaceutically acceptable to the mammal in the sense that any deleterious side effects which may occur in the mammal are transient in nature and do not give r se to a disease state. A preferred solvent system which has been found to give rise to extracts of galega officinalis having a weight reducing effect is the solvent system 50% ethanol: 50% H 2 0. Generally, extracts of Galega officinalis in solvent systems should be physiologically acceptable to the recipient mammal and substantially non-toxic thereto. Extracts of Galega officinalis can be prepared as follows. Galega officinalis plant material (e.g. leaves, stems, shoots, flower parts) can be dried, then pulverised to form a powder. A desired amount of the powdered plant material can then be extracted to exhaustion using conventional solvent extraction technology, for example, first with ethyl acetate followed by polar organic solvent (e.g. 50% polar organic solvent (e.g. ethanol): 50% H,0) . The extraction can be by 'cold percolation or by soxhlet. In a further aspect of the invention there is provided use of an organic solvent extract of Galeσa officinalis comprising galegine and/or at least one polar organic solvent as a weight reducing agent in a mammal. In a preferred aspect of the invention the extract of Galeσa officinalis for use as a weight reducing agent in a mammal comprises a polar organic solvent and water. Preferably, the extract comprises a solvent system of 50% ethanol: 50% H0. Galegine and related compounds can also be chemically synthesised. Generally, compounds of the invention may be synthesised according to procedures as outlined below. For Example, 7,7 Dimethylallylamine can be synthesised according to the following procedure : Amine 4 can be synthesised using the Gabriel synthesis of primary amines (ref. 2) . Potassium phthalimide 1 can be treated with dimethyl allyl bromide 2 to form the N-alkylphthalimide 3. Subsequent treatment with hydrazine hydrate and hydrochloric acid can afford the target molecule 4. Phenylmethylguanidine hydrocnloride can be synthesised according to the following procedure: Aminoiminomethanesulfonic acid 6 - synthesised from formamidinesulfonic acid 5 (ref. 3) - can undergo nucleophilic displacement of HS0 3 ~ upon treatment with benzyl amine 7. The monosubstituted guanidine 8a can then be subsequently transformed to the solid hydrochloride salt 8b. Allylguanidine sulphate may be synthesised according to the following outline: H Target r.olecule 11 can be obtained via nucleophilic displacement of methane thiol from S-methyl isothiourea sulphate 10 by allyl amine (ref. 4) . 7,7-Dimethylallylamidine hydrochloride may be synthesised as follows: 12 13 1i» N-monoalkylated amidines are formed from N-unsubstituted amidines via a transa ination reaction (ref. 5). Thus, reaction of formamidine hydrochloride 13 with γ,γ-dimethylallylamine 12 can afford the N-substituted amidine 14. The viscous oil is subsequently transformed to the picrate salt for structural analysis via conventional techniques. References herein to weight reduction are to be construed to include either a reduction of the subject's actual weight after administration of a compound of formula (I) , or a reduction in rate of weight gain in comparison to that of an untreated subject. Subjects to be treated by the present invention include both human and animal (e.g. ' dog, cat, horse) subjects and are preferably mammalian subjects. The weight reducing agent is administered in an amount effective to result in weight reduction. The weight reducing agent is preferably administered in a total amount per day of not more than 40 g/kg body weight, more preferably not more than 20 mg/kg body weight and most preferably not more than 4 mg/kg. With respect to minimum dose, the weight reducing agent is preferably administered in a total amount per day of at least 0.02mg/kg, most preferaoiy at least 0.2 mg/kg. The weight reducing agent may be administered once or several time a day. The compounds of formula (I) and formula (II) may be administered per se or in the form of physiologically acceptaole salts. Sucn physiologically acceptable salts include, but are not limited to, those prepared from the following acids: hydrochloric, hydrochloric, hydrobromic, sulphuric, nitric, phosphoric, aleic, acetic, salicylic, p-toluenesulphonic, tartaric, citric, isethionic, methanesulphonic and benzenesulphonic. Also, pharmaceutically acceptable salts, such as sodium, potassium, or calcium salts may be used. The compounds of formula (I) and/or formula (II) may be administered as pharmaceutical formulations, both for veterinary and for human medical use, which comprises at least one weight reducing agent together with one or more pharmaceutically acceptable carriers and optionally any other therapeutic ingredients. The carrier(s) must be pharmaceutically acceptable in the sense of being compatible with the other ingredients of the formulation and not unduly deleterious to the recipient thereof. The formulations include those suitable for oral, rectal or parenteral (including subcutaneous, intramuscular or intravenous) administration. Formulations for oral administration are preferred, particularly as solid or liquid food formulations. The formulations may be prepared by any of the methods well known in the art. All methods include the step of bringing the compound of formula (I) and/or formula (II) into association with a carrier which constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active compound into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product into desired formulations. Formulations of the present invention suitable for oral administration may be prepared as loose powder or granules; or in unit dosage forms as discrete units such as capsules, sachets, tablets or lozenges, each containing a predetermined amount of the weight reducing agent as a powder or granules; or a suspension in an aqueous liquor or non-aqueous liquid such as a syrup, an elixir, an emulsion or a draught. A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine, with the active compound being in a free-flowing form such as a powder or granules which is optionally mixed with a binder, disintegrant, lubricant, inert diluent, surface active agent or dispersing agent. A syrup may be made by adding the active compound to a concentrated aqueous solution of a sugar, for example sucrose to which may also be added any accessory ingredient(s) . Such accessory ingredient(s) may include flavourings, suitable preservatives, an agent to retard crystallisation of the sugar, and an agent to increase the solubility of an other ingredient, such as a poiyhydric alcohol, for example glycerol or sorbitol. Formulations for parenteral administration conveniently comprise a sterile aqueous preparation of the active compound, which is preferably isotonic with the blood of the recipient. Formulations for rectal administration may be presented as suppository vith a suitable carrier such as cocoa butter, or hydrogenated rats or hydrogenated fatty car oxylic acids. In addition to the aforementioned ingredients, the formulations zi this invention may further include one or more accessory ir.gredient(s) selected from diluents, buffers, flavouring agents, binders, disintegrantε, surface active agents, thickeners, lubricants, preservatives (including antioxidants) and the like. The following Figures and Examples are provided to illustrate the present invention, and should not be construed as limiting thereof. Figure li Effect of different dietary concentrations of galegine on various parameters in normal mice, la: Daily body weight (each point is mean ±SE of 7 observations) . Effect of treatment * P<0.05, ** P<0.01, two-way ANOVA for repeated measures. lb: Daily food intake, (lb: ( each value is the mean per mouse for each cage) . Figure 2: Effect of different dietary concentrations of galegine on various parameters in genetically obese (ob/ob) mice. 2a: Daily body weight (each point is mean ±SEM of 7 observations) . Effect of treatment ** P<0.01, two-way ANOVA for repeated measures. 2b: Daily food (2b: each value is the mean per mouse for each cage) . Figure 3: Effect of the 50%-ethanol-water cold extract of galega officinalis en various parameters in normal mice. la: Daily body weight .each point is mean ±SEM of 7 - 8 observations). Effect of treatment ** P<0.01, two-way ANOVA for repeated measures. 3b: Daily food intake. ** P<0.01 compared to control, Student's unDaired t-test. Figure 4 ; Effect of the 50%-ethanol - water soxhlet extract of Galega officinalis on various parameters in normal mice (8 day study) . 4a: Daily body weight (each point is mean ±SEM of 7 - 8 observations). Effect of treatment ** P<0.01, two-way ANOVA for repeated measures. 4b: Daily food intake. ** P<0.01, Student's unpaired t-test. Figure 5: Effect of the 50%-ethanol - water soxhlet extract of Galega officinalis on various parameters in normal mice (14 day study) . 5a: Daily body weight (each point is mean ±SEM of 7 observations). Effect of' treatment ** P<0.01, two-way ANOVA for repeated measures. 5b: Daily food intake. ** P<0.01, Student's unpaired t-test. Figure 6: 400.14 MHz proton NMR, water supressed spectrum of the ethanol-water extract of Galeσa officinalis. Residual ethanol signals *. Examples 1, and 6 to 8 inclusive illustrate further compounds of Formula (1) . Examples 4 and 6 to 3 inclusive illustrate further compounds of Formula (1) . Example l Weight Reducing Effect of Gaieσine on Normal Mice Galegine was synthesised by and obtained from Aston Molecules Limited. This example shows that when galegine is administered to mice, as part of their feed, the mice do not gain weight to the same extent as untreated mice. This experiment consists of four treatments, as follows: (1) 160 mg of galegine was added to 500 g of mice feed; (2) 240 mg of galegine was added to 500 g of mice feed; (3) 320 mg of galegine was added to 500 g of mice feed; (4) a negative control group to which no galegine was added. The procedure for this experiment is as follows. Each group of mice (7 mice per group) were allowed to feed and drink freely. The experiment was carried out for 14 days. Body weight and intakes of food were measured daily. The results of the experiment are shown in Figure 1. It can be seen that all treated groups, showed a sharp drop in food intake on Day 1. All groups then showed a gradual return towards the control level, with the 160 and 240 mg/500 g groups showing a transient increase in food intake above the control level. Mean body weight in all treated groups was found to be lower than that of the untreated control group. The 320 mg/500 g group showed the greatest reduction in weight gain over the 14 day period. The mice did not display overt symptoms of steatorrhoea and symptoms of diarrhoea were not observed. Example 2 Weiσht Reducing Effect of Galeσine on Genetically Obese <Ob/ob Mice This experiment was carried out in the manner described in Example 1 with the exception that the mice to be treated were genetically obese (ob/ob) mouse strain, and data was collected for 28 days. The results of this experiment are shown in Figure 2. It can be seen that all treated groups showed a sharp drop in food intake on Day 1. Food intake remained below the levels of the control group for the duration of the experiment. Mean body weight in all treated groups was found to be lower than that of the untreated control group. The 320 mg/500 g group showed the greatest reduction in weight gain over the 28 day period. The mice did not display overt symptoms of steatorrhoea and no symptoms of diarrhoea were observed. As in Example 1 no overt symptoms of steatorrhoea and symptoms of diarrhoea were not observed. Example 3 A mixture of 4-bromo-2-methyl-2-butene (Aldrich) (5.51g; 37mmol) , potassium phthalimide (Aldrich) (7.4lg; 40 mmol) and dimethylforma ide (40cm 3 ) was heated at 115°C for 1.5h. Dichloromethane and water were added to the cooled reaction mixture and extracted with 2x15 cm 3 portions of chloroform. The combined organic extracts were washed with 0.2 N sodium hydroxide (20cm J ) and vater (20cm J ) , then dried (Na : S0,) , filtered and concentrated m vacuo, to afford a colourless crystalline solid (6.55g; 32%) . An analytically pure sample was obtained following recrystallisation from absolute alcohol, m.p. 99 - 101°C (Lit 6 . 100°C) Found : C, 72.32; H, 6.02; N, 6.44% Calc. for C 13 H !3 NO- : C, 72.54; H, 6.09; N, 6.51% a (KBrJ/cn.- 1 : 3185, 3172, 3107, 3068, 3025 (Ar-Hstr) , 2960, 2904, 2829, 2789, 2613, 2588 (Al,Hstr) , 1589, 1562, 1547, 1475 (c=cstr & c=ostr) . 5H (250MHz, CDC1 3 ) 1.70 (3H, s, CH 3 ) , 1.83 (3H, S, CH 3 ) , 4.25 (2H, d, J72HZ, CH,-) , 5.26 (IH, , vinyl C-H), 7.67-7.72 (2H, m, aryl C-H), 7.78-7.34 (2H, m, aryl C-H). Example 4 7-7-Dimeτhylallylamine A mixture of N-(7, -dimethylallyl) -phthalimide (Example 3) (3.0g; 13.9mmol), ethanol (10cm 3 ) and 35% hydrazine hydrate (0.89g; 15.2mmol), was heated under reflux for 2h, cooled, then treated with 10M hydrochloric acid (1.52cm J ; 15.2mmol) and filtered. The collected phthalhydrazide was triturated with water, filtered and the combined filtrates concentrated in vacuo. The residue was treated with a solution of 85% potassium hydroxide (l.lg; 16.4mmol) m water (5cm 3 ) and extracted with diethylether. The combined extracts were dried over potassium hydroxide and distilled through a fractionating column. The fractions collected between b.p. 96-93°C were treated with hydrochloric acid etherate to afford a colourless powder (0.90g; 53%) , m.p. 200 - 201°C fLit 0 .p. 201°C) . Found : C, 48.92; H, 10.10; N, 11.24%. MH + , 124.0572, 122.0650 Calc. for CjH !2 Cl : C, 49.33; H, 9.95; N, 11.52%. M, 123.0629, 121.0658 vmax (KBrJ/cm 1 : 3200-2890 (N-Hstr) , 2777, 2690, 2615, 2562 (Al- Hstr) , 1681 (N-Hdef) , 1597 (C=Cstr) . όΗ [250MHz, (CD 3 ) 2 SO] 1.64 (3H, s, CH,) , 1.70 (3H, s, CH 3 ) , 3.34 (2H, d, J7.2HZ CH,) , 5.23 (IH, br t, vinyl C-H), 8.21 (3H, br s, exch, NH 3 ) . Example 5 Aminoiminomethanesulfonic Acid To a stirring suspension of formamidinesulfonic acid (Aldrich) (3.24g; 30mmol) in acetic acid (lOcirr), in an ice-bath, peracetic acid (CAUTION, 36-40% in acetic acid, 7.5cm 3 , 39mmol) was added dropwise at such a rate that the temperature was maintained below 20°C. After addition was complete, the mixture was stirred at room temperature for 16h. During reaction the fine white suspension was replaced by larger crystals of product, which were removed by filtration and washed with absolute alcohol to give (3.46g, 93%) colourless crystals, m.p. 121 - 122°C (dec). The product was used in subsequent reactions without further purification. Example 6 Phenylmethylguanidine Hydrochloride Equimolar amounts of benzyl amine (Aldrich) (1.07g; lOmmol) and a suspension of aminoiminomethanesulfonic acid (Example 5) (1.24g; lOmmol) were stirred in absolute methanol (5cm 3 ) at room temperature until the mixture became clear (5min) and then stirred for a further 3h. The solvent was removed under reduced pressure to afford a viscous residue. Subsequent trituration with diethylether - to remove unreacted benzyl amine - was followed by addition of dichloromethane and cold 2M sodium hydroxide. The dichloromethane layer was dried (Na,S0 4 ) , then concentrated in vacuo. HC1 etherate was added to the colourless oil to produce a colourless solid (0.647g; 35%), m.p. 169 - 171°C. Found : C, 51.49; H, 6.59; N, 22.48%. M^-Cl, 150.1092. Calc. for CH,,N,Cl : C, 51.75; H, 6.51; N, 22.63%. M, 187.0690, 135.0720. <SH [250MHz, ;CD,),SO] 4.40 (2H. d, J6.2HZ, PhCK,-) , 7.1 - 7.8 (9H, m & [br s], exch. , N-H and aryl C-H) , 3.29 (IH, m, aryl C-H) . I/max (KBr)/cm- " : 3400 - 3000 :-H str and Ar-H str) , 2981, 2939, 2924, 2373, 2348, 2761, 2696 Al-H str) , 1971, 1955, 1894, 1378, 1334, 1817 (C=N str) , 1634 - 1610, 1567 (N-H def and C=C str) . Allylguanidine Sulphate Allylamine (Aldrich) (2.85g; 0.05mmol) and 2-methyl-2- thiopseudourea sulphate (Sigma Chemical Co.) (5.22g; 37.5mmol) in water (30cm 3 ), were heated under reflux for 2.5h, under nitrogen. The colourless solution was evaporated to dryness in vacuo to yield colourless crystalline material. Recrystallisation from ethanol/water (2:1) afforded crystalline needles (3.71g; 67%), m.p. 208 - 210°C. Found : C, 32.43; H, 6.96; N, 28.44; S, 10.92%. MH", 297.1362. Calc. for C,H :0 N 6 O 4 S ' : C, 32.42; H, 6.80; N, 28.36; S, 10.82%. M, 296.1267. 5H (250MHZ, CF,CO,H) 3.77 (4H, M, CH,) , 5.26 (4H, M, vinyl C-H), 5.74 (2H, M, vinyl C-H). vmax (KBr)/c ; - 3450 - 3100 (N-H str), 3083, 3016 (vinyl C-H str), 2983, 2935, 2377, 2831, 2722 (Al-H str), 2034, 1967, 1363 (C=N str) , 1670 - 1550 (N-H & C=C str) . Example 8 Ύ.-,—Dimeτhviailylamidine Hydrochloride Formamidine hydrochloride (Aldrich) (0.57g; 7.05mmol) was suspended in absolute alcohol (8cm 3 ) and nitrogen introduced above the liquid. 7,7-Dimethylallyl amine ^0.6g; 7.0mmol) was added and the reaction mixture stirred for 20 minutes at room temperature, then heated under reflux for 2 hours. After cooling, the ethanol was removed under reduced pressure and the residue triturated with diethyl ether to remove any unreacted 7,7-dimethylallylamine. The residue was concentrated in vacuo to produce a yellow-orange viscous oil. A small quantity of the crude product was taken up in water and treated with hot aqueous picric acid. The resulting picrate salt was used to identify the amidine's structure. 1. Hydrochloride Salt δU [250MHz, (CD 3 ) 2 SO] 1.64 (3H, S, CH 3 ) , 1.69 (3H, S, CH 3 ) , 3.87 (2H, d, J6.95 Hz, CH,) , 5.25 (IH, M, vinyl C-H), 7.75 - 8.10 (IH, m, amidine C-H 9.0 - 9.7 ' (2H, br m, N-H), 9.95 (IH, br s, N-H). cmax (LF)/cm :" : 3400 - 2800 (N-H str & C-H str), 1947 (C=N str), 1705, 1633, 1537 (N-H def & C=C str). 2. Picrate Salt Found : C, 42.46; H, 4.42; N, 19.88%. C,,H 15 N 5 θ, requires : C, 42.23; H, 4.43; N, 20.52%. όΗ [250MHz, .CD iSO] 1.65 (2H. S, CH,) , 1.71 ;3H, S, CH,) , 3.3 - 3.95 (2H, M, CH,) 5.13 ( IH, . vinyl C-H) , 7.92 (IH, m, vinyl C- H) , 3.4 - 9.3 (3H, br , excr., N-H) 3.59 (2H, s, aryl C-H). 7max (KBr)/cm-: 3401, 2213 , -H str) , 2129, 3099, 3089, 2060 (vinyl C-H str), 2971, 2941, 2919 (Al - H str) , 1708, 1670, 1643, 1604, 1560 (C=C str & N-H def) . References 1. D. Semenow, C-H. Shih and W.G. Young, J. Am. Chem. Soc. , 1958, 80., 5472-5. 2. J.D. Roberts, R.H. Mazur, J. Am. Chem. Cos, 1951, 73 / 2509- 23. 3. K. Kim, Y-T. Lin and S. Mosher, Tetrahedron Lett., 1988, 2_9, 3183-3186. 4. C.E. Braun, J. Am. Chem. Soc, 1933, 5_6, 1280-84. 5. P. Reynauld, J-D. Brion and G. Menard, Bull. Soc. Chem. Fr., 1973, 11-449-56. 6. G. Desvages and M. Olomucki, Bull. Soc. Chem. Fr. , 1969, 9., 3229-32. Example 9 Preparation cf Extracts of Galega A number of extracts of galega were incorporated into the diet in an amount equivalent to 10% w/w of the dried plant material in the diet. Thus, 50g of powdered galega was extracted to exhaustion, first with ethyl acetate followed by 50% ethanol - water, extraction was by cola percolation or by soxhlet, using methods known in the art. Groups of normal mice received extract-containing diet or control diet for periods of 7 - 14 days. Preparation cf Diet Containing Galegine Diets containing galegine at concentrations of 160, 240, 320, and 640 g per 500 g, or control diet were administered to groups of normal or genetically obese (ob/ob) mice for periods of 7 - 28 days. Measurements In all studies, body weight and intakes of food and water were measured on a daily basis. At the end of the treatment period serum glucose and insulin levels were determined. Spectroscopic and Chromatographic Analysis of 50% ethanol - Water Extracts 400 MHz H NMR and Η-'H-cosy 45 were performed on the cold ethanol - water extract. Thin layer chromatography (TLC - silica-gel) was performed on cold and soxhlet ethanol - water extracts. The solvent system used was chloroform (6.5) - methanol (3.5) - water (1.0). The plate was developed with Sakaguchi reagent which gives a reddish - orange colour with galegine. Results Studies with Extracts of Galeσa The cold and soxhlet 50%-ethanol - water extracts contained compoundfs) vhich caused significant and sustained weight loss (P<0.01) (Figures 3, 4 and 5). Food intake was initially depressed (Figures 3a, -b and 5b) but returned to the control level within 2 - 7 days. As in Examples 1 and 2 overt symptoms of steatorrhoea and symptoms of diarrhoea were not observed. The -H NMR spectrum of the 50%-ethanol - water cold extract (Figure 6) showed the presence of glycose (sugar) - type compounds (c.a. δ 3.5 - 5.0), aromatic - type compounds ( δ 6.8 - 8.0) as well as galegine (c.a. δ 5.24, 3.78, 1.68 and 1.75). TLC was performed with the 50%-ethanol - water extracts and with pure galegine. On development of the plate, the extracts produced reddish - orange spots with very similar Rf values to that of pure galegine (approximately 0.7).

Claims: Cl ims 1. Use of a compound of formula (I) : n wherein R 1 R is selected from the σroup phenyl and C = CR 3 — R 1 , R 2 and R 3 are independently selected from the group H, C,^ alkyl, C-^ alkenyl, and C,^, alkynyl; R 4 and R J are independently selected from H, C,^ alkyl, C j ^ alkenyl, and C,. 6 alkynyl; NH R' is selected from the group H or C - R° wherein R" is selected from the group H, NH, and C,_ 6 alkyl; n is a whole integer from 0 to 6; and physiologically acceptable salts thereof in the manufacture of a composition for weight reduction. Use according to claim 1 of a compound of formula (I) wherein R' , R 2 , R\ R' and R' are independently selected from the group H, C,._, alkyl, 2,, alkenyl and C,., alkynyl; R' is selected from the group NH,, H, C, . alkyl; and n is a whole integer from 0 to 2; and physiologically acceptable salts thereof. 3. Use according to claim 1 of compounds according to formula (II): n wherein R 1 , R 2 , R 3 , R 4 and R 5 independently selected from hydrogen, C,_ 5 alkyl, C-^ alkenyl, C 2J5 alkynyl and NH,; and n is from 0 to 6; and physiologically acceptable salts thereof. 4. Use according to claim 3 of a compound of formula (II) wherein R 1 and R 2 are both C,^ alkyl or C,. 6 alkenyl or C^ alkynyl. 5. Use according to claim 3 or claim 4 of a compound of formula (II) wherein R 1 and R 2 are both C,. 2 alkyl, or C,. 2 alkenyl or C,., alkynyl or mixtures thereof. 6. Use according to claim 1 or claim 2 of a compound of formula ;II) wherein R' and R 2 are independently selected from C.j, alkyl; R 3 is selected from H or C ,, alkyl; R 4 and R-" are independently selected from or C,. ft alkyl; and n is 1 or 7. Use of galegine as a weight reducing agent in a mammal. 8. Use of galegine as a weight reducing agent in a human. 9. Use of galegine [ (3-methyl-2-butenyl) guanidinej in the manufacture of a composition for weight reduction. 10. Use of an organic solvent extract of Galeσa officinalis comprising galegine as a weight reducing agent in a mammal. 11. Use according to claim 10 in a human. 12. Use according to claim 10 or claim 11 wherein the organic solvent extract comprises galegine and at least one polar organic solvent. 13. Use according to any of claims 10 to 12 wherein the organic solvent comprises a polar organic solvent and water. 14. Use according to any of claims 10 to 13 wherein the organic solvent comprises 50% ethanol: 50% H,0. 15. The compound 7,7-dimethylallylamidine. 16. A pharmaceutical formulation comprising a compound of formula . I) in admixture with a pharmaceutically acceptable carrier. 17. A pharmaceutical formulation according to claim 16 comprising a compound of formula (II) in admixture with a pharmaceutically acceptable carrier. 18. A formulation according to claim 16 or claim 17 comprising a compound of formula (II) wherein R 1 and R 2 are independently selected from C,_s alkyl; R 3 is selected from H or C 1JS alkyl; R 4 and R 3 are independently selected from H or C,_ j alkyl; and n is 1 or 2. 19. A formulation according to any of claims 16 to 18 wherein the compound is galegine in admixture with a pharmaceutically acceptable carrier. 20. A method of attaining weight reduction is a mammal which comprises administering to the mammal an effective, non- toxic amount of a compound of formula (I) : n wherein R R is selected from the group phenyl and C I = CR J - where R 2 R 1 , ? and R- ! are independently selected from the group H, C,. 6 alkyl, C,^ alkenyl, and C,^ alkynyl; R 4 and R 3 are independently selected from H, C,^ alkyl, C,^ alkenyl, and C^ alkynyl; NH R' is selected from the group H or C - R° is selected from the group H, NH 2 and C,^ alkyl; n is a whole integer from 0 to 6; and physiologically acceptable salts thereof in the manufacture of a composition for weight reduction. 21. A method of attaining a weight reduction in a mammal which comprises administering to the mammal an effective, non- toxic amount of a compound of formula (II) n wherein R l , R 2 , R ; , R 4 and R 3 independently selected from hydrogen, C,. 6 alkyl, ,. 6 alkenyl, C,. 6 alkynyl and NH,; and n is from 0 to 6; and physiologically acceptable salts thereof. 22. A method according to claim 20 or claim 21 wherein R' and R ; are independently selected from C,.„ alkyl; R 3 is selected from H or C,. Λ alkyl; R 4 and R' are independently selected from H or C,. 6 alkyl; n is 1 or 2. 23. A method according to any of claims 20 to 22 wherein the compound is galegine. 24. A method of attaining a weight reduction is a mammal which comprises administering to the mammal an effective, non- toxic amount of an organic solvent extract of Galega officinalis. 25. A method according to claim 24 wherein the organic solvent comprises at least one polar organic solvent. 26. A method according to claim 25 wherein the organic solvent comprises ethanol arid water. 27. A method according to claim 26 wherein the organic solvent comprises 50% ethanol: 50% H 2 0.

Inventors: FURMAN BRIAN LAURENCE, GRAY ALEXANDER IRVINE, PALIT PIALI, WAIGH ROGER DAVID, FURMAN, BRIAN, LAURENCE, GRAY, ALEXANDER, IRVINE, PALIT, PIALI, WAIGH, ROGER, DAVID
Applicants: FURMAN BRIAN LAURENCE, GRAY ALEXANDER IRVINE, PALIT PIALI, UNIV STRATHCLYDE, WAIGH ROGER DAVID, FURMAN, BRIAN, LAURENCE, GRAY, ALEXANDER, IRVINE, PALIT, PIALI, UNIVERSITY OF STRATHCLYDE, WAIGH, ROGER, DAVID
Application year: 1996
Main classification: A61K31
IPC codes: A61K31, A61P3, C07C257, C07C279