FUCOPEPTIDES

Abstract: Compounds of formula (I) wherein R is CH3 and R1 is a peptidic residue or R1 is OH and R is a peptidic residue have pharmacological activity as sialyl Lewis X mimetics, e.g. in the prevention or treatment of disorders or diseases which are mediated by the binding of selectins in cellular adhesion.

Description: Fucopeptides The present invention relates to fucopeptides, a process for their production, their use as a pharmaceutical and pharmaceutical preparations containing them. It is widely accepted that a family of receptors, the selectins, are involved in the recognition of various circulating cells by the endothelium and platelets and play a role in certain diseases including cancer, autoimmune disorders, inflammation, atherosclerosis and blood clotting. There are three known members of this family: L-selectin, P-selectin and E-selectin. E-selectin (also designated endothelial leukocyte adhesion molecule, ELAM-1) is a cell surface protein inducibly expressed in endothelial cells. For example, its production is increased on vascular endothelial cells when adjacent tissue has been damaged or invaded by a microorganism. E-selectin recognizes sialyl Lewis X (SLe*) which is a cell surface carbohydrate ligand found on neutrophils and monocytes, anchored onto the outer membrane thereof by integral membrane glycoproteins and/or glycolipids. SLe x mediates binding of neutrophils and monocytes to the activated vascular endothelial cells by binding to E-selectin, so that these leukocytes may diffuse into the damaged tissue. However, there are many situations in which the recruitment of leukocytes by adhesion to the endothelial cells is abnormal and in excess, and the end result is tissue damage instead of repair. Although SLe" has been considered to be potentially useful as an anti-inflammatory agent and its synthesis on large scales has been developed for clinical evaluation, this natural saccharide can only be used as an iηjectable form in cases presenting with acute symptoms as it is orally inactive and has a short half-life in blood. Thus, there is a need for compounds which can interfere with binding of the ligands to the selectins and prevent the initial cellular adhesion process. According to the invention, there is provided a compound of formula I wherein i) R is CH 3 , and either R, is a radical of formulae (a,) or (a 2 ) (a,) wherein m is 2 or 3; n is 2 or 3; M is a cation; R 2 is H or a saturated or unsaturated hydrocarbon residue with up to 20 carbon atoms, optionally bearing in ω position a formyl or a C M alcohol acetal or C 2 . 4 diol acetal group; R 3 is H, -CH 2 OH or -CH 2 CH 2 OH; and R 4 is H, C alkyl, -CH 2 OH, -CH 2 CH 2 OH or -CH 2 CH 2 CH 2 OH with the provisos that 1) one of R 3 and R 4 is H, and 2) when R 4 is H, R 3 is -CH 2 OH or -CH 2 CH 2 OH, and 3) when R 3 is H, R 4 is CH 3 , -CH 2 OH, -CH 2 CH 2 OH or -CH 2 CH 2 CH 2 OH; 2Σ R, is a radical of formula (b) wherein R s is MOOC - CH, -r- CH- CO- NH- (b,) (b 2 ) (b 3 ) or MOOC— CH- CH 2 ' ι CO— NH wherein p is 1 or 2; q is 2 or 3; r is 1 or 2; R 6 is H, NH 2 or -NHR. wherein R. is an amino protecting group; R 7a is -CH 2 OH, -CH 2 CH 2 OH or -CH(OH)-CH 2 OH and R 7b is H or each of R 7a and R^ is CH 2 OH; R π is H or -OH; R, 3 is -(CH 2 ) r COOM or -SO 3 M wherein j is 1, 2 or 3; and M is as defined above; the second hydroxy substituent of the phenyl group in (b 4 ) being in either meta position; Q R, is a radical of formula (c) O-CH - CH- | 3 (c) R 9 - CO - NH - CH - COR β wherein R g is OM|, OR, 4 , R s -R p or -NHR y wherein M, is a cation, R 14 is a saturated or unsaturated hydrocarbon residue, R s is a spacer group, R p is a phosphatidyl residue and R y is a saturated or unsaturated lipophilic residue; and R, is MOOC - CH- CH 2 ) S - CO - NH - R- (c 2 ) (c,) 0-C,- 4 alkyi (C3) (c 4 ) wherein s is l or 2; t is l or 2; v is 2 or 3; M, RA J , R, I and R I3 are as defined above; and R l0 . is -CH : OH, -CH 2 CH : OH or -CH(OH)-CH 2 OH and R l0b is H or each of R IOa and R IOb is CH 2 OH; the second hydroxy substituent of the phenyl group in (c 2 ) being in either meta position; or wherein ii) R, is OH, and R is a radical of formula (d) R 6 ^a I H I (d) MOOC-f- CH 2 - - CH - CO- N- C - CO- NH - CH 2 - I R 12b wherein w is 1 or 2; R 12i is -CH(OH)-(CH 2 ) x -OH and R 12b is H or each of R 12a and R 12b independently is -CH 2 OH or -CH 2 CH 2 OH; x is 2 or 3; and R 6 and M are as defined above. M may be H* or any salt forming cation for a carboxy or sulfate which maintains the water-solubility of the compound of formula I, e.g. a monovalent or one equivalent of a polyvalent cation, for example an alkali metal ion such as lithium, sodium or potassium, an alkaline earth cation such as calcium or magnesium, as well as zinc, iron and aluminium ions and the ammonium (NH + 4 ) ion. M is preferably H + , Li * or Na * . It is preferred that the cation M be a pharmaceutically acceptable cation. When the cation is polyvalent, an appropriate number of molecules of formula I or a mixture of compounds of formula I and one or more appropriate anions such as acetate, chloride, carbonate and the like are also present. M, may independently have one of the significance given above for M, preferably identical to M. When R x is an amino protecting group, it may be such a group as disclosed in "Protective Groups in Organic Synthesis" T.W. Greene, J. Wiley & Sons NY, 2nd ed., chapter 7, 1991, and references therein, preferably a pharmaceutically acceptable amino protecting group, particularly tert.-butoxy-carbonyl or benzyloxycarbonyl. When R 2 in the radical of formula (a,) is a saturated or unsaturated hydrocarbon residue, it may be e.g. C, .20 alkyl, C 2.20 alkenyl or C 2 . 20 alkynyl. Examples of R 2 bearing a formyl group include e.g. 2-oxo-ethyl, 3-oxo-propyl, 5-oxo-pent-3-enyl, 8-oxo-octyl and 10-oxo-dec-4-enyl. When R 2 is an alkyl or alkenyl acetal group formed from a C M alcohol or C^diol, it may be e.g. any of the acetals preparable from the above aldehydes using C M alcohols or C^diols, e.g. methanol, ethanol, isopropanol, sec.-butanol, n-butanol, ethylene glycol, propylene glycol, 2,3-butanediol,l ,4-butanediol or 1,3-butanediol. R 2 is preferably H. Any C alkyl as R 4 in the radical of formula (a,) is preferably CH 3 . When R g is R,-R p or NHR y , the resulting compound of formula I may be or is suitably utilized in a liposomal preparation as part of the liposome membrane as a means for administering said compound. The spacer group R s is a residue which links the carbonyl to the oxygen of the phosphatidyl residue, e.g. a hydrocarbon residue. The phosphatidyl residue is a glycerophosphate esterified with saturated and/or unsaturated fatty acids, e.g. myristic, palmitic, stearic, palmitoleic or oleic acid. R y may be a saturated or unsaturated aliphatic residue optionally comprising or interrupted by a functional group e.g. -CO-, e.g. a residue based on a dicarboxylic acid diester bearing saturated or unsaturated fatty aliphatic residues. R y is preferably wherein each of X and Y independently is C 8 . 20 alkyl or C 8 . 20 alkenyl and Z is a bridging group, e.g. a polyethyleneoxy group, preferably a poly-(3-50, preferably 3-15)-ethylene- oxy group, or a -C, .4 alkylene-CO- or -phenylene-CO-group. In the compounds of formula I, the following significances are preferred either individually or in any combination or sub-combination: 1. R is CH, and R, is a radical of formula (a,). 2. In the radical of formula (a,) R 2 is H. 3. In the radical of formula (a,) R 3 is H and R 4 is -CH 2 OH. 4. In the radical of formula (a,) R 3 is H and R 4 is CH 3 . 5. R is CH 3 and R, is a radical of formula (b). 6. In the radical of formula (b), R s is a radical (b,). 7. In the radical (b,) R 7a is CH 2 OH or CH(OH)-CH 2 OH and R^ is H. 8. In the radicals (a 2 ) and (b 3 ), the group -OC M alkyl is preferably OCH 3 . 9. R is CH j and R, is a radical of formula (c). 10. In the radical of formula (c) R g is C,. 6 alkoxy, preferably methyl or ethyl. 1 1. In the radical of formula (c), R 0 is a radical (c,). 12. In the radical (c,), R IOa is CH(OH)-CH 2 OH and R 10b is H. 13. In the radical of formula (c), R, is a radical (c 2 ). 14. In the radical (c 2 ), R 6 is NH 2 . 15. R, is OH and R is a radical of formula (d). 16. In the radical of formula (d), R 12a is CH(OH)-(CH 2 ) x -OH and R 12b is H. The compounds of formula I may comprise one or more asymetric carbon atoms. It will be understood that the present invention includes all individual isomeric forms, enantiomers and diastereoisomers as well as mixtures, e.g. racemates, unless otherwise stated. In the radical of formula (a,), the asymetric carbon atom bearing R 3 has preferably following configuration: In the radical of formula (b,), when the carbon atom bearing R 7ϊ and R 7b is asymetric (i.e. K-n, is H), it preferably has following configuration: * 7a V H The same applies to the asymetric carbon atom bearing R 10a when R 10b is H in residue (c,). In the radicals (b 2 ) and (c 3 ), the pyrrolidinyl moiety preferably has following configuration: In the radicals (b 4 ) and (c 2 ) the asymetric carbon atom bearing the substituted benzyl moiety preferably has following configuration: The radical of formula (c) has preferably following stereochemistry: COOR 8 R 9 - CO - HN In the radical of formula (d), when R 12b is H, the asymetric carbon atom bearing R 12a has preferably following configuration: H R i 2a » ^ C A N H The present invention also includes a process for the production of the compounds of formula I. They may be produced by analogy to known methods. The compounds of formula I may be produced for example by removing at least one protecting group which is present in a compound of formula I in protected form, e.g. amino and/or hydroxy protected form. The compounds of formula I in protected form are mainly compounds wherein the hydroxy group(s) present in the fucose moiety is (are) protected. Groups which can be employed in the present invention to block or protect the hydroxy group are well-known to those skilled in the art and, preferably, said groups can be removed, if desired, by methods which do not result in any appreciable destruction of the remaining portion of the molecule, for example, by chemical or enzymatic hydrolysis, treatment with chemical reducing agents under mild conditions, irradiation with ultraviolet light or catalytic hydrogenation. Hydroxy -protecting (blocking) groups which are advantageously used are those which are common in carbohydrate chemistry especially for primary alcohols, secondary alcohols and vicinal cjs_ and trans diols. Suitable hydroxy-protecting groups may be, for example, acyl groups such as acetyl, trichloroacetyl, phenoxycarbonyl, benzyloxy carbonyl, benzhydryloxycarbonyl, trityl- oxycarbonyl and 2,2,2-trichloroethoxycarbonyl, ether groups such as methoxymethyl, benzyloxy methyl, allyl, benzyl, p-methoxybenzyl, p-nitrobenzyl, benzhydryl, trityl or triorganosilyl groups such as tri(C,-C 6 ) alkylsilyl (e.g. trimethylsilyl, triethylsilyl), tri- isopropylsilyl, isopropyldimethylsilyl, t-butydimethylsilyl, methyldiisopropylsilyl or methyldi-t-butylsilyl), t-butyl-diphenylsilyl, triarylsilyl (e.g. triphenylsilyl, tri-p-xylylsilyl) or triaralkylsilyl (e.g. tribenzylsilyl). Examples of these and other suitable hydroxy- protecting groups e.g. for the protection of 1,2- or 1 ,3-dihydroxy groups, for example cyclic ether groups such as optionally substituted methylene acetal or ethylidene acetal and methods for their formation and removal are known in the art, e.g., see Protective Groups in Organic Synthesis, second ed., T.W. Greene and P.G.M. Wuts, John Wiley & Sons, New York, 1991, Chapter 2 and references therein. Compounds of formula I wherein R 6 is NHR. may also be converted in compounds of formula I wherein R 6 is NH 2 by removal of the amino protecting group R x . The compounds of formula (I) thus obtained may be recovered in free form or in salt form. Compounds of formula I in protected form wherein R is CH 3 and R, is a radical of formula (a), used as starting materials may be produced, e.g. as indicated in Scheme 1. Scheme 1 X is a hydroxy protecting group, e.g. as indicated above. R' is a leaving group, e.g. an amino protecting group, for example Boc or Fmoc. Preferably the starting materials are used as specific enantiomers in order to obtain the compounds of formula I with the desired configuration. Compounds of formula I in protected form wherein R is CH 3 and R, is a radical of formula (b) used as starting materials may be prepared, e.g. as indicated in Scheme 2. Scheme 2 Compound I coupling R y is a leaving group, e.g. halogen, preferably F. Step ii is a reduction which is intended to include well-known reduction procedures for the azido group such as reaction with a phosphine, e.g. triphenylphosphine, or a hydride, e.g. lithium aluminium hydride. Step iii is an amide bond coupling e.g. as known in the art of peptide chemistry. Compound II may be selected e.g. from Asp-Ser-OH, Glu-Ser-OH, Glu-(α-hydroxymethyl)Ser-OH in pro.ected form, e.g. Compound 10. Step iii may also comprise coupling with an appropriate amino acid in protected form followed by reacting with a diacid derivative as disclosed in Scheme 1. Compounds of formula I in protected form wherein R is CH 3 and R, is a radical of formula (c) may be prepared e.g. as indicated in Scheme 3. Scheme 3 Compound IV R_ a ' s C,^alkyl. R, is an amino protecting group. Compound IV may be selected e.g. from 2-amino-3,4-dihydroxy-butyric acid, α-hydroxymethyl serine or Glu-Tyr-OH in protected form, e.g. (2S,3R)-N-Boc-2-amino-4-benzyloxy-3-hydroxybutyric acid. R^CO is the amino acid residue (optionally completed with a diacid residue) of Compound IV. Steps i) and ii) are amide bond coupling reactions effected according to standard procedures. Step ii) may also comprise coupling with an appropriate amino acid in protected form followed by reacting with a diacid derivative, e.g. as disclosed in Scheme 1. Step ii) may further also comprise the conversion of -COOR gϊ into -COR g , e.g. into an acid, acid salt, lipophilic ester or lipophilic amide, e.g. as disclosed in Examples 44 and 45. Compound HI may be used in the form of one or another of the individual enantiomers or in the form of mixtures. Above Scheme 3 illustrate the preparation of a compound of formula I wherein R is CH 3 and R, is a radical of formula (c) with the preferred configuration. Compounds of formula I in protected form wherein R, is OH and R is a radical of formula (d) wherein R 12a is CH(OH)-(CH 2 ) x -OH, used as starting materials, may be produced e.g. as indicated in Scheme 4. Scheme 4 Preferably 2 vicinal X groups (hydroxy protecting groups) form together H 3 C on the : fucose moiety. H 3 C The above reactions may be effected in analogy with known methods, e.g. as described in the following examples. Insofar as the production of the starting materials is not particularly described, the compounds are known or may be prepared analogously to methods known and practiced in the art. The following examples are illustrative of the invention. All temperatures are in °C. Following abbreviations are used: Example 1 : Compound 6 is deprotected by treatment with hydrogen gas over a palladium/carbon catalyst, followed by treatment with sodium methoxide to give the sodium salt of Compound 7. Η NMR (500 MHz, D 2 O) δ 1.16 (d, J = 6.3 Hz, 3 H), 1.75-1.92 (m, 4H), 2.35 (m, 1 H), 3.20 (m, 1 H). 3.43 (m. IH). 3.55 (dd. J = 6.0, 12.0 Hz, 1 H), 3.67 (dd, J = 3.0, 12.0 Hz, 1 H). 3.73 (m. 2 H). 2.84-4.02 (m, 4 H); 13 C NMR (125 MHz, D 2 O) δ 16.6, 21.6, 24.5, 34.3, 35.4, 37.5, 56.5, 63.4, 68.2, 68.7, 70.8, 71.9, 72.6, 74.4, 166.8, 172.5, 177.0; electrospray mass m/z 423 [(MH) + ; calculated for C I7 H 3I O 10 N 2 : 423]. Compound 6, used as starting material, may be prepared as follows: a) Fucose tetraacetate is treated with allyl trimethyl silane and boron trifluoride etherate in dry acetonitrile at room temperature to give Compound 2 (the ratio α:β is greater than 10:1). Kozikowsky, A.P. and Sorgi, K.L. Tetrahedron Lett. (1983), 24: 1563. b) Compound 2 is ozonolyzed by reaction with ozone in the presence of triphenyl phosphine. The product aldehyde is then subjected in situ to reductive amination by treatment with hydrogen gas over a palladium carbon catalyst in the presence of ammonium acetate to give the corresponding fucose tetraacetate bearing a 2-amino-ethyl group (Compound 3). c) Compound 3 as obtained above is coupled with (lS,2R)-2-N-Boc - amino- 4-benzyloxy-3-hydroxy butyric acid (prepared from glycine and O-benzylglyco- aldehyde by a threonine aldolase-catalyzed reaction according to Wong et al. Tetrahedron Lett. 1995, 26, 4081 ) in l -(3-dimethylaminopropyl)-3-ethylcarbodiionide hydrochloride solution to form the amide Compound 5 d) The protecting group is removed from Compound 5 by treatment with ethyl acetate in acidic solution (4N HC1). The deprotected product is then treated in situ with glutaric anhydride (in triethylamine buffer) to obtain Compound 6. By following a procedure in analogy with that of Example 1 above, the compounds of formula wherein R,, R 2 , n and m have the significances as indicated in Table 1 below, may be prepared. Table 1 Ex. m By following a procedure in analogy with that of Example 1 but using the appropriate starting materials, the compound of Example 6 may be obtained: Example 7: 11 12 Compound 1 1 (122 mg, 0.12 mmol) is dissolved in methanol (2 mL), Pd(OH) 2 on carbon (20 mg) is added and the mixture is stirred under hydrogen (1 atm) for 12 hours. The catalyst is filtered through celite and the product is purified by silica gel chromatography (CHCl 3 /methanol, 3:1) and biogel P2 chromatography (H 2 O). Compound 12 is obtained after purification. 'H NMR (500 MHz, D 2 O) δ 1.48 (d, 3H, J = 7.0 Hz,H-6), 1.47-1.74 (m, 4H), 1.74 (s, 9H), 2.00-2.11 (m, 3H), 2.47 (m, IH), 3.64 (s, IH, H-4), 3.76 (m, IH), 3.97-4.25 (m, 6H, H-2, H-3, H-5), 4.65-4.74 (m, 2H), 5.31 (d, IH, J = 3.5 Hz, H-l). ,3 C NMR (125 MHz, D 2 O) δ 15.80, 23.73, 24.51, 28.18, 30.18, 31.60, 39.68, 49.47, 53.42, 55.89, 55.99, 62.39, 67.12, 68.42, 70.08, 72.39, 94.38, 94.65, 170.9, 171.1, 174.7, 178.1. HRMS calcd for C 24 H 4I N 3 O 12 Cs (M+Cs+) 696.1745, found 696.1717. Compound 11, used as starting material, may be prepared as follows: a) 2.3,4-Tri-O-benzyl-L-fucopyranose is dissolved in anhydrous dichloromethane at 0° and DAST (1.06 g, 0.0066 mol) is dropped in. After the mixture is stirred at 0° for 30 min., the reaction is quenched by the addition of water. The aqueous solution is extracted with dichloromethane and the organic fractions are combined, dried over MgSO 4 and filtered. The solvent is evaporated and the fluoride is used for the glycosylation without further purification. 4A Molecular sieves, tin (II) chloride (1.67 g, 0.0088 mol) and silver perchloride ( 1.82 g, 0.0088 mol) are added to a solution of the fluoride in anhydrous dichloromethane (20 mL) at 0°. The mixture is stirred for 5 min. and (R,R) azidocyclo- hexanol (0.93 g, 0.0066 mol) is added. The reaction is warmed to room temperature and stirred for 6 hours. After filtration through celite, the filtrate is concentrated and applied to silica gel chromatography (hexane/ethylacetate, 8: 1). Compound 8 is obtained as a clear oil. b) Compound 8 (1.3 g, 2.33 mmol) is dissolved in tetrahydrofuran (10 mL, containing 1 % H 2 O) and triphenylphosphine (638 mg, 2.56 mmol) is added. The mixture is stirred at room temperature for 5 h. After evaporation of the solvent, the residue is purified by chromatography on a silica gel column with CHCl 3 /methanol (50: 1 —> 30: 1 ). Compound 9 is obtained as a syrup. BnOOC 10 c) O-Benzyl-N-Boc-L-aspartic acid is dissolved in anhydrous dichloromethane and EDAC (640.3 mg, 3.34 mmol) and N-hydroxysuccinimide (384.4 mg, 3.34 mmol) are added. The mixture is stirred at 4° for 12 h and the solvent is evaporated. O-benzyl- N-Boc-aspartic N-hydroxy succinimide ester is obtained by silica gel chromatography (ethylacetate/hexane, 1 :1.5 — > 1 :1). O-Benzyl-N-Boc-aspartic N-succinimide ester (603 mg, 1.44 mmol) and O-benzyl -serine (281 mg, 1.44 mmol) are dissolved in DMF (2 mL) and Et 3 N (1 mL) is added. The mixture is stirred at room temperature for 1 hour. After evaporation of the solvent and silica gel chromatography (CHC1 3 ) the product 10 is obtained. d) Acid 10 (141 mg, 0.29 mmol), EDAC (81 mg, 0.42 mmol) and HOBT (57 mg, 0.42 mmol) are dissolved in dichloromethane (2 mL) at room temperature and stirred for 5 min. before compound 9 (148 mg, 0.56 mmol) is added. The mixture is stirred at room temperature for 3 hours and the solvent is evaporated. The residue is applied to a silica gel column (hexane/ethylacetate, 1.5: — > 1 :1) and compound 1 1 is obtained as a syrup. Η NMR (500 MHz, CDC1 3 ) δ 1.20 (d, 3H, J = 6.5 Hz, H-6), 1.08-1.60 (m, 4H), 1.44 (s, 9H), 1.73 (m, IH), 1.94 (m, 3H), 2.71 (m, IH), 3.05 (m, IH), 3.65 (dd, IH, J = 3.5, 9.5 Hz), 3.76 (m, IH, H-4), 3.89 (dd, 1 H, J = 3.0, 9.5 Hz), 3.98 (dd, IH, J = 2.5, 10.0 Hz, H-3), 4.04 (dd, IH, J = 3.5, 10.0 Hz, H-2). 4.10 (m, I H, H-5), 4.44 (m, IH), 4.61 (m, IH), 4.68 (m, IH), 4.95 (d, IH, J = 3.5 Hz, H-l ), 4.45-5.17 (m, 10H), 5.68 (m, IH), 7.33 (m, 25H). 13 C NMR (125 MHz, CDC1 3 ) δ 16.72, 23.34, 23.82, 28.20, 36.29, 36.59, 50.42, 50.65, 52.45, 52.58, 52.82, 66.69, 66.72, 66.78, 69.26, 73.09, 73.18, 74.80, 75.98, 75.98, 77.73. 79.35, 94.52, 128.0 ( ), 135.2, 135.3, 137.5, 183.6, 139.0. 155.3, 169.6, 170.2, 170.6, 171.9. HRMS cacld for C 59 H 71 N 3 O 12 Cs (M+Cs+) 1 146.4092, found 1 146.4035. By following a procedure in analogy with that of Example 7 above, but using the appropriate starting materials, the following compounds of formula wherein R 5 is as indicated in Table 2, may be prepared. Table 2 Ex. R 5 HRMS Calc. Found HO ^CH j OH ^CH [M + Cs + ] H T 611.1217 611.1241 HO,C-f-CH 2 -) — CO-N -CH- CH j OH CH [M + Cs + ] H T HO.CH-CH 2 CO 611.1217 611.1241 ■ N — CH- CH OH H * *> ' 485.2111 485.2127 NaO-C-f-O- - - -CO-N -CH- CH j OH OH ^CH^ 11 H T [M + Cs + ] HO.CH-CH 2 -^-CO-N -CH- 625.1373 625.1355 CH.OH .OH CH 3 H f NaO j C • CH 2 ■ CH-CO-N-CH— [M + Na + ] A 516.2169 516.2169 NH. HO j CH-CH 2 -)-C IO 26 Table 2 (continued) Ex. R< HRMS Calc. Found .1546 OH CH, H0 2 C- H f ■ CH, CO-N-CH— Compound of Example 11: ,H NMR < 500 M ^ D *°) * "° 3H, J = 6.5 Hz. H-6).1.20 (m, 4H), 1.75 (m.6H).2.16 (m, 1H).2.56 (t.2H. J = 7.5 Hz).2.33 (m.2H), 3.41 (m.1H), 3.43 (m, 2H), 3.63 (m, 2H. H-2 and H-3), 3.69 (m, 1H. H-4), 3.78 (m, 1H. H-5), 4.09 (ddd, 1H, J = 2.5, 6.0, 8.5 Hz), 4.46 (d, 1H, J = 2.5 Hz).5.02 (d.1H, J = 3.0 Hz. H-1). «C NMR (125 MHz, D 2 0) δ 15.85, 21.96, 23.71, 24.60, 28.84.31.41.35.38, 35.45. 53.67, 55.19, 62.73. 67.13, 68.26. 69.88.71.65.72.22, 75.19. 93.31. 172.3, 176.6. Compound of Example 12: iH NMR (500 MHz, D 2 0) δ 1.12 (d. 3H, J = 6.5 Hz. H-6), 1.08-1.26 (m, 4H). 1.39 (s. 9H). 1.65-1.80 (m, 3H). 2.14 (m, 1H). 2.74 (m. 2H), 3.39-3.88 (m. 9H). 4.37 (m. 2H), 4.98 (d, 1H, J = 3.5 Hz, H-1). "C NMR (125 MHz. D 2 0) δ 15.82. 23.70. 24.58. 28.03. 29.30. 31.78, 37.32. 52.05. 53.40, 55.55. 62.93, 67.14. 68.32, 69.95, 71.99. 72.25, 76.23, 82.21 , 94.06. 157.6, 170.8, 173.4, 175.8. Compound of Example 13: iH NMR (500 MHz, D 2 0) δ 1.15 (d. 3H. J = 6.5 Hz, H-6), 1.10-1.40 (m. 4H), 1.66-1.85 (m, 3H), 2.16 (m, 1 H). 2.59 (dd. 1 H. J = 8.5. 17.5 Hz). 2.72 (dd. 1 H, J = 5.0. 17.5 Hz). 3.40 (m. 1 H). 3.51 (dd. 1 H. J = 6.0. 12.0Hz), 3.56 (dd. 1 H. J = 3.5, 10.0 Hz, H-3). 3.60 (m, 1H). 3.64 (dd. 1 H, J = 4.0. 10.0 Hz, H-2), 3.69 (m. 1 H, H-4), 3.73 (m. 1 H), 3.85 (m, 1 H). 3.91 (m. 1H. H-5), 4.15 (m, IH), 4.40 (d. 1 H. J = 7.0 Hz). 5.00 (d. 1 H. J = 4.0 Hz, H-1). «C NMR (125 MHz, D 2 0) δ 15.77. 23.69. 24.62, 29.06, 31.80. 38.41 , 51.47, 53.40, 55.59. 62.76. 67.09. 68.35. 69.91. 71.79. 72.24. 75.68. 93.63, 170.6, 176.8. 176.8. Example 18: The benzyl groups of 15 are cleaved by hydrogenation according to procedure of Example 5. Compound 16 (amorphous): Η NMR (400 MHz, D 2 O) δ 4.98 (d, J = 3.2 Hz, 1 H, H-l), 4.61 (d, J = 2.2 Hz, 1 H), 4.56 (d, J = 7.2 Hz, 1 H), 4.46-4.44 (dd, J = 2.2 and 6.4 Hz, 1 H), 4.28^.13 (m, 3H), 4.02-3.98 (m, 1 H). 3.86-3.59 (m, 5 H), 2.42-2.22 (m, 4 H), 1.90-1.79 (m, 2 H), 1.30-1.18 (m, 9H); electrospray negative ion mass (declustering potential = -80 V) m/z 523 [(M-H); calcd for C 21 H 36 N 2 O 13 : 524]. Compound 15, used as starting material, may be prepared as follows: BnO OBn a) OBn 13 L-Fucose is first converted to tribenzylfucosyl phosphite according to Wong et al. J. Org. Chem. 1994, 52. 864. The resulting compound (1.0 equivalent) is coupled to Boc-L-Thr-OEt (1.1 equivalents) using trifluoromethanesulfonic acid (0.1 equivalent) as catalyst in methylene chloride at 0° to give compound 13 after standard workup and purification. 14 First Boc deprotection of 13 in 30 % TFA in 0.1 M CH 2 C1 2 at 25°, 30 min; quench water, wash NaHCO 3 , dry sodium sulfate; purification by flash chromatography gives the corresponding free amine. This amine (1.0 equivalent) is coupled with 1.1 equivalents of (2S,3R)-N-Boc-2-amino-4-benzyloxy-3-hydroxy-butyric-acid using 1.5 equiv of EDC1, 1.5 equiv of HOBt. 0.1 M CH 2 C1 2 , 0°, 30 h, to provide 14 after standard workup and flash column chromatography purification conditions. c) Boc deprotection of 14 in 30 % TFA, 0.1 M CH 2 C1 2 at 25°, 30 min is followed by quench with water, wash with NaHCO 3 and drying over sodium sulfate. Purification by flash chromatography is followed by coupling with 1.1 equivalents monobenzyl glutarate. 1.5 equiv of EDC1, 1.5 equiv of HOBt, CH 2 C1 2 , 25°, 20 h, to provide compound 15. Compound 15: 'H NMR (400 MHz, CDC1 3 ) δ 8.00 (d, J = 9.1 Hz, 1 H), 7.21-7.50 (m. 25H), 6.75 (d, J = 7.6 Hz, 1 H), 5.12-4.57 (m, 1 1 H), 4.35-3.99 (m, 7H). 3.80-3.60 (m. 5 H), 2.45 (t. J = 7.5 Hz, 2H), 2.43 (t, J = 7.0 Hz, 2H), 1.98 (t. J = 7.5 Hz. 2H), 1.20-1.26 (m, 6 H). 1.06 (d, J = 6.4 Hz, 3 H); HRMS for Cs+ (M+Cs+), calcd 1 107.3614, found 1 107.3667. By following a procedure in analogy with that of Example 18 above but using the appropriate starting materials, the compounds of formula wherein R g and R, are as defined in Table 3, may be prepared. Table 3 Ex. R. MS calc. found CH 2 OH 19 H CH 2 OH ES (M-H-) HO,C— t-CH. CO - NH V ^ - C — 496 495 0 Li * UO.C--CH,-^- NH 2 Table 3 (continued) Ex. R g R, MS calc. found H0 2 C- -CH 2 -HCO 3 H0 2 C-fCH 2 * *-*CO Table 3 (continued) Ex. R, R, MS calc. found Example 29 19 20 A solution of glycopeptide 19 (23.7 mg, 40 μmol) is stirred in 90 % TFA in water (1 mL) at room temperature. After 3 hrs., the reaction solution is evaporated down under reduced pressure and azeotroped twice with toluene (2 x 5 mL). An nmr on the crude product is performed to ensure that the isopropylidene moieties are removed and the product is then taken on to the next step without purification. The crude glycopeptide is then hydrogenated according to the procedure of Example 5 to give a white solid 20: R f (4: 1 : 1 nBuOH:H 2 O:HOAc) 0.29: 'H nmr (400 MHz; D 2 O) 5.23 (d, J 3.7, Hl α ), 4.55 (dd, J 7.9 and 4.7, Hl β ). 4.37-4.32 (m, H2' (α + P) ), 4.15-4.05 (m, H5 α + H3' p ), 3.95-3.92 (m, H4 α + H3' 0 ), 3.86 (d, J 3.4, H4 p ), 3.83 (dd, J 10.3 and 3.2 H3 α ), 3.78 (dd, J 10.3 and 3.7, H2 α ), 3.74-3.68 (m, H5 p ) 3.64-3.61 (m, H3 p + C6Η 2(α + β) ), 3.55-3.44 (m, H2 p + C6H a H Wα + P) ), 3.42-3.31 (m, C6H.H ,. + P) ), 2.62-2.48 (m, C2"H 2(α + P) ) + C3"H 2(α + p) ) , 1.76-1.47 (m, C4Η 2(α + p) ) + C5Η 2(α + p) ); ,3 C nmr (100 MHz; D 2 O) 182.93, 179.1 1, 175.20, 174.66, 98.86, 94.80, 75.19, 75.08, 75.02, 74.21, 72.91, 71.78, 71.48, 71.28, 71.18, 70.71, 70.52, 63.84, 63.80, 61.00, 60.90, 60.48, 42.15, 42.03, 34.71, 34.19, 34.12. 31.84. 31.44, 31.14: High Resolution Mass Spectrum (Doped with Nal): Found M + Na, 447.1570. C, 6 H 28 N 2 O π requires M + Na, 447.1591. Compound 19, used as starting material, may be prepared as follows: 17 EDCI (95.4 mg, 500 μmol) is added to a stirred solution of 6-amino-6-deoxy-l ,2,3,4-diisopropylidene-α-L-galactopyranoside (130 mg, 500 μmol), (2S,3RS)-2-N-Boc-amino- 6-benzyloxy-3-hydroxy-hexanoic acid (177 mg, 500 μmol), HOBT (68 mg, 500 μmol) and 4-methyl morpholine (108 μL, 1000 μmol) in dry DMF (5 mL) under argon at -20°. The resulting mixture is stirred at -20° for 1 hr and then allowed to warm slowly to room temperature. After 14 hours, the reaction solution is quenched 5 % w/v citric acid solution (20 mL) and extracted with ethyl acetate (6 x 25 mL). The combined organic extracts are washed with saturated sodium hydrogen carbonate solution (50 mL) and saturated sodium chloride solution (50 mL), dried (MgSO 4 ) and evaporated down under reduced pressure. The residual oil is purified by flash chromatography (silica gel, using gradient elution 40% — > 50% — > 66% ethyl acetate in hexane) to give 17 as a pale yellow foam: R f (75 % ethyl acetate in hexane) 0.66. b) 18 A solution of the glycopeptide (17) (59.5 mg, 100 μmol) in 15 % v/v trifluoroacetic acid in dry 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)4-H-pyran (1 mL) is stirred under argon at room temperature. After 2 hr., the solution is evaporated down under reduced pressure and the residual oil dissolved up in n-butanol:water:methanol (5:3:2) (10 mL). To the solution is added Dowex (Cl " , prewashed with methanol, 100 mg) and the mixture stirred for 30 mins., then filtered and the solid washed with methanol (3 x 5 mL) and the combined filtrate and washings evaporated under reduced pressure. The residual oil is purified by flash chromatography (silica gel, using gradient elution 19:0.9:0.1 → 9:0.9:0.1 DCM:MeOH:NH 3 (ιq) ) to give 18 as a light brown oil: R f (9:0.9:0.1 DCM:MeOH:NH 3 q) ) 0.42. c) Succinic anhydride (6.0 mg, 60 μmol) is added to a stirred solution of amino glycopeptide 18 (27.7 mg, 56 μmol) in methanol (1 mL) at room temperature. After 1 hr, the solution is evaporated down under reduced pressure. The residual solid is purified by flash chromatography (silica gel, using gradient elution 5 -→ 10% acetic acid in ethyl acetate) to give compound 19 as a pale yellow gum. R f (10 % acetic acid in ethyl acetate) 0.49: IR (Film) cm-1 3303, 2980, 2935, 1644, 1558, 1436, 1382, 1255, 1211, 1 167, 1 109, 1070, 1006, 901: Η nmr (400 MHz; CD 3 OD) 7.32-7.23 (5H, m, aromatic), 5.44 (IH, d, J 5.0, 2 x HI), 4.59 (IH, dd, J 7.9 and 2.1, 2 x H3), 4.48 (2H, s, 2 x CH 2 Ph), 4.38-4.34 (IH, m, 2 x H'2), 4.31 (I H, dd, J 5.0 and 2.4, 2 x H2) 4.21 (IH, d, J 7.9, 2 x H4), 4.00-3.89 (1.5H, m, 2 x H5 + H3'), 3.84-3.76 (0.5H, m, H3'), 3.52-3.46 (3H, m, 2 x C6H. H b + 2 x C6Η 2 ), 3.27-3.20 (IH, m, 2 x C6 H, H b ), 2.56-2.48 (4H, m. 2 x C2"H 2 + 2 x C3"H 2 ), 1.80-1.54 (4H, m, 2 x C4Η 2 + 2 x C5'H 2 ), 1.45 (3H, s, acetonide Me), 1.40 (3H, s, acetonide Me), 1.32 (3H, s, acetonide Me).1.29 (3H, s, acetonide Me): 13 C nmr (100 MHz; CD 3 OD) 137.21, 126.78, 126.24, 126.03, 107.86, 107.34, 95.19, 71.24, 70.06, 69.56, 69.28, 69.24, 68.58, 64.68, 56.66, 38.02, 28.96, 28.35, 24.54, 23.83, 23.75, 22.62, 21.99: High Resolution Mass Spectrum (Doped with Csl): Found M + Cs, 727.1875. C 29 H 42 N 2 O requires M + Cs, 727.1843. By following a procedure in analogy with that of Example 29 but using the appropriate starting materials, the compounds of formula wherein R A and R B are as defined in Table 4, may be prepared. Table 4 OH CH-f-CH^-OH HO 31 H0 2 C- ■ CH, ■ CO - NH - CH OH CH CH, OH HO 32 HO-C— CH 2 ->— CO - NH - CH • OH CH-(-CH 2 -) -OH HO 33 HO j 2 C* -T- ■ C wHπ 2 , -CO-NH-CH- OH CH- CH.-)— OH HO 34 OH i HO— CH 2 -^-CH-CH- ■••HI OH HO,C-f-CH,-)-CO-NH Table 4 (continued) Ex. R. R 36 HO ■ CH 2 OH CH I OH CH- H0 2 C- (CH- - CO • 37 HO, CH 2 OH CH OH CH- HO.C - CH, - CH - CO - N A NH 2 8 HO ^CH 2 OH CH OH 9 (CH 2 ) 3 -OH * / CH *w OH I CH H0 2 C-CH 2 -CH-CO-N ✓ A H NH, Table 4 (continued) Ex. R A R B 40 OH HO-CH, CH j OH \> V H0 2 C- (CH 2 ) 2 - CO - NH - C - 41 OH HO-CH 2 CH,OH H0 2 C - (CH 2 ) 3 CO - NH - C - 42 CH 2 OH HO-CH 2 ^ CHi -vvw OH H0 2 C - (CH 2 ) 2 - CO - NH - C ■ CH 2 OH 3 OH HOCH 2 CH- HO z C - (CH 2 ) 3 - CO - NH - C - Example 44 27: R' = Bn 28: R' = H To the solution of compound 27 (91.4 mg, 59.2 μmol) in MeOH (5 mL) is added Pd(OH) 2 on carbon (20 mg). The mixture is stirred for 2 days under hydrogen balloon. The catalyst is filtered off and the filtrate is evaporated in vacuo. The residue is applied to silica gel column chromatography (CHCl 3 :MeOH = 1 : 1) and then sephadex LH20 gel filtration chromatography (eluted with MeOH) to obtain the desired amphiphile 28. Η NMR (500 MHz, CD 3 OD) d 0.80 (6H, t, J=6.5), 1.16 (6H, d, J=5.5), 1.23-1.41 (52H, m), 1.60-1.71 (4H. m), 2.04-2.17 (2H, ), 2.31-2.60 (6H, m), 2.66-2.77 (2H, m), 2.97-3.09 (2H. ), 3.31-3.40 (3H, m), 3.50-3.71 (14H. m). 3.79-3.87 (I H, m), 3.88-3.98 (I H, m), 4.04-4.26 (4H, m), 4.44-4.56 (3H, m), 4.95 (IH, brs). HRMS calcd for C 62 H l l4 N 4 O 17 Cs (M+Cs) 1319.7233, found 1319.7264. Compound 27, used as starting material, may be prepared as follows: a) Compound 21 A suspension of dibenzyl 2,3,4-O-tribenzyl-α-L-fucopyranosyl phosphite (138 mg, 0.204 mmol), N-Fmoc threonine allyl ester (77.5 mg, 1 eq) and molecular sieve 4A (470 mg) is stirred for 18 hr at room temperature. To the mixture is added a solution of TMSOTf (13.6 mg, 0.3 eq) in CH 2 C1 2 (1 mL) at -15°. After stirring for 2 hr at the same temperature, saturated NaHCO 3 aq is added to quench and the mixture is diluted with CH 2 C1 2 . The organic lay is separated, dried over MgSO 4 and evaporated in vacuo. The residue is applied to silica gel column chromatography (Hex.EtOAc = 4: 1 ) to obtain the compound 21. Compound 22 To a solution of compound 21 (104 mg, 0.13 mmol) in CH 2 C1 2 (5 mL) is added Et 2 NH (0.72 mL, 53 eq) and the mixture is stirred for 18 hr at room temperature. The solvent and reagent are removed in vacuo and the residue is purified by silica gel column chromatography (CH 2 Cl 2 -CH 2 Cl 2 :MeOH=100:3) to obtain the compound 22. Compound 23 To a solution of the compound 22 (242 mg, 0.42 mmol) and N-benzyl glutarylamide-L-proline (135 mg, 1 eq) in CH 2 C1 2 (5 mL) is successively added HOBT (86 mg, 1.5 eq) and EDC (105 mg, 1.3 eq) at 0°. The reaction mixture is stirred for 20 min at 0° and the temperature is allowed to rise to room temperature within 18 hr. The reaction mixture is evaporated in vacuo and the residue is dissolved with EtOAc. The EtOAc solution is washed with 1 N HCl, saturated NaHCO 3 and brine successively. The organic layer is dried over MgSO 4 and evaporated in vacuo. The residue is applied to silica gel column chromatography (toluene ~toluene:acetone=2: l) to obtain the compound 23. Compound 24 To a solution of the compound 23 (169 mg, 0.192 mmol) and morpholine (167 mg, 10 eq) in THF (10 mL) and DMF (1 mL) is added Pd(PPh 3 ) 4 (23 mg, 0.1 eq) under argon atmosphere at room temperature. After stirring for 24 hr, the reaction mixture is evaporated in vacuo. The residue is dissolved with EtOAc and washed with I N HCl. The organic layer is separated, dried over MgSO 4 and evaporated in vacuo. The residue is purified on silica gel column chromatography (CH 2 Cl 2 ~CH : Cl 2 :MeOH 10: 1 ) to obtain the compound 24. Synthesis of lipid part Compound 25 To a solution of oxalyl chloride (1.54 mL, 2N CH 2 C1 2 solution) is added a solution of DMSO (277 mg, 1.5 eq) in CH 2 C1 2 (3 mL) and a solution of 2-[2-(2-azidoethoxy)- ethoxy] ethanol in CH 2 C1 2 (5 mL) each 2 min interval at -78°. After the mixture is stirred for 30 min at the same temperature, Et 3 N (1.64 mL) is added to the mixture at -78° and the temperature is allowed to rise to room temperature within 1 hr. The reaction mixture is poured into ice water and extracted with CH 2 C1 2 . The organic layer is washed with IN HCl and saturated NaHCO 3 successively, dried over MgSO 4 and evaporated in vacuo. The crude aldehyde is used without further purification. To a solution of the crude aldehyde and r,3'-dicetyl-L-glutamate p-toluenesulfonic acid salt (500 mg, 1 eq) in THF (3 mL) and MeOH (9 mL) is added NaCNBH 3 (41 mg) at 0°. The mixture is stirred at 0° for 1 hr and the temperature is allowed to rise to room temperature within 18 hr. The solvent is evaporated in vacuo and the residue is dissolved with CH 2 C1 2 . After the solution is washed with 1 N HCl, the organic layer is dried over MgSO 4 and evaporated in vacuo. The residue is applied to silica gel column chromatography (Hex-EtOAc=4:l) to obtain compound 25. Compound 26 To the solution of compound 25 (320 mg, 0.427 mmol) in MeOH (13 mL), THF (3 mL) and IN HCl (1.3 mL) is added Pd on carbon (99 mg). The mixture is stirred for 2 days under hydrogen balloon. The catalyst is filtered off and the filtrate is evaporated in vacuo. To the residue is added saturated NaHCO 3 and the mixture is extracted with EtOAc. The organic layer is dried over MgSO 4 and evaporated in vacuo to obtain compound 26. Coupling between sLe' mimetic and Lipid Compound 27 To a solution of compound 24 (94.6 mg, 0.1 13 mol) and compound 26 (81.7 mg, 1 eq) in CH 2 C1 2 (5 mL) is added HOBT (23 mg, 1.5 eq) and EDC (29 mg, 1.3 eq) successively at 0°. The reaction mixture is stirred at 0° and the temperature is allowed to rise to room temperature within 20 hr. The reaction mixture is evaporated in vacuo and the residue is dissolved with EtOAc. After the solution is washed with saturated NaHCO 3 , the organic layer is dried over MgSO 4 and evaporated in vacuo. The residue is purified on silica gel column chromatography (toluene:acetone=l : l) to obtain compound 27. iH NMR (500 MHz, CDC1 3 ) δ 0.88 (6H, t, 1=7.0), 1.12 (3H, d, J=6.0), 1.17 (3H, d ϋ^lx 21 ' 1 41 (52H « m >> 1*57-1-65 (4H, m), 1.88-1.97 (2H, m), 2.12-2.20 (IH, m) 2.22-2.29 (IH, m), 2.38 (2H, t, J-7.0). 2.45-2.83 (5H, m). 3.18-3.27 (2H, m), 3 42- 3.59 (10H, m), 3.63 (IH, ore), 3.66 (IH, dd, J=11.0. 4.0), 3.82 (IH, dd, J=10 3 ' 3 0) 3.84 (IH, q, 1=7.0), 4.01-4.13 (4H, m). 4.48-4.58 (4H, m . 4.61 (IH, d J=ll 5) 4 64 (IH, d, J=11.5), 4.69 (IH, d, J=11.5), 4.74 (IH, d, J=11.5), 4.75 (IH, d, J=l l 5) 4.92 (IH, d, J=11.5), 4.96 (IH, d, J=3.5). 5.06 (2H, s), 7.10 (IH, brt, J=3.0), 7 25- 7.43 (20H, m), 7.87 (IH, t, J=9.0). m/z C9 0 Hi3 8 N 4 Oi7 1548 (M+H) Example 45 By following a procedure in analogy with that of Example 44 but using the appropriate starting materials, the following compound may be obtained J H NMR (500 MHz, CDCI3) d 4.80 (d, J-=2.6 Hz. IH), 4.46 (1, J=8.5 Hz, IH), 4.42-4.40 (m, 4H), 4.05-3.95 (m, 6H), 3.76-3.43 (m, 6H), 2.23 (t, J=7.7 Hz, 2H), 2.30-2.20 (m. 3H). 2.12-2.01 (m, 2H), 1.98-1.91 (m, 4H), 1.85-1.77 (m. IH). 1.57-1.50 (m. 4H), 1.25-1.13 (br.. >50H), 1.11 (d, J=6.3 Hz, 3H). 1.09 (d. J=6.6 Hz, 3H), 0.78 (t, J=6.7 Hz, 6H); MS m/c calc'd for C59Hi06 4θl6Cs (M4-Cs + ): 1259.6658. found 1259.6620 The compounds of formula I and their pharmaceutically acceptable salts exhibit pharmaceutical activity and are, therefore, useful as pharmaceuticals. In particular, they inhibit adhesion between cells containing a selectin such as E-selectin on their surfaces and effector cells such as neutrophils or HL-60 cells that have SLe * on their cell surfaces, or a synthetic poly-SLe x -product or poly-SLe'-product. More particularly, the compounds of formula I inhibit sLe* binding to E-selectin as indicated in the following test methods: a) Preparation of a soluble form of E-selectin. The extracellular domain of E-selectin, fused to the constant region of the K light chain (mCK) is cloned into the baculovirus shuttle vector pVL941 (Invitrogen) and expressed in SF-9 cells. The soluble fusion protein is purified by affinity chromatography using the rat anti-mouse CK monoclonal 187.1 antibody coupled to Sepharose. Cellular Binding Assays. The compounds of formula I are assayed for their ability to block the adhesion of HL-60 cells to E-selectin immobilized onto 96 well plates. Rat anti-mouse CK antibody is added to 96 well plates (20 μg/ml in carbonate/bi- carbonate buffer, pH 9.5) and incubated overnight at 4°C. The plates are blocked with 3 % BSA in assay buffer (20 mM HEPES, pH 7.4 containing 150 mM NaCl and 1 mM CaCl 2 ) for 8 hours at room temperature and washed 3x with assay buffer. E-selectin mouse CK fusion protein (10 μg/ml in assay buffer) is added and incubated for 2 hours at 37°C or overnight at 4°C. The plates are washed 3x with assay buffer. Then the compound to be assayed is added and pre-incubated for 15-30 min at 37°C. 1 x 10 s labelled HL-60 cells in assay buffer are transferred to each well and allowed to adhere to the E-selectin for 30-45 min at 37°C. Then the plates are gently washed 3-4x with assay buffer to remove unbound cells. Adherent cells are quantified by measurement of fluorescence (Cytofluor 2350 system). Fluorescent labelling of HL-60 cells with BCECF-AM: HL-60 cells are cultured in Iscove medium supplemented with 20 % FCS, glutamine and non essential amino acids. One day before the experiment is performed, the cells are subcultured (l xl0°c/ml). The cells (1 x 10 6 c/ml) are labelled by incubating with 5 μg/ml BCECF-AM (diluted from stock in DMSO) for 20 minutes at 37°C in PBS. Materials: ELISA plate: Nunc Immuno Plate MaxiSorp (439454) HL-60 cells: obtained from ATTC catalogue Affinity purified E-selectin: each batch of E-selectin is tested functionally to determine the appropriate concentration for use in the assay. Fluorescent dye: bis-carboxyethyl-carboxyfluorescein acetoxy methyl ester (BCECF-AM) available from Molecular Probes. In this assay, compounds of formula I inhibit adhesion of HL-60 cells to E-selectin, at a concentration of from 0.3 to 10 mM. Compounds of Examples 12, 13 and 18 inhibit adhesion of HL-60 cells to E-selectin at a rate of 100 % and 80 % (for the 2 later) in a concentration of 10 mM. b) sLe a -polymer/E-selectin cell free binding assay. Rat anti-mouse CK antibody (10 μg/ml in carbonate/bicarbonate buffer, pH 9.5), prepared from rat hybridoma cell line 187.1 (ATCC) is coated onto microtiter plates overnight at 4°C. The plates are washed two times with assay buffer (20 mM HEPES, pH 7.4 containing 150 mM NaCl and 1 mM CaCl 2 ), blocked for 8 hours at 4°C with 3 % BSA in assay buffer and washed 3 times. To each well E-selectin mouse CK fusion protein (3 μg/ml in assay buffer) is added followed by an incubation overnight at 4°C. A complex is formed between biotinylated sLe a -polymer (polyacrylamide-type glycoconjugate, Syntesome GmbH, Munich, Germany) containing 20 % mol sLe* (= 0.81 μmol sLeVmg polymer) and streptavidin-peroxidase (Boehringer Mannheim, Germany) by incubating 20 μl of sLe a -polymer (1 mg ml) with 80 μl streptavidin-peroxidase, 20 μl fetal calf serum and 80 μl assay buffer without CaCl 2 for two hours at 37°C (the pre-formed sLe s -polymer/streptavidin-peroxidase complex is stable over several months at 4°C). The complex is then diluted 3:10'000 in assay buffer and added to the E-selectin coated wells together with the compound to be assayed. The complex is allowed to bind for two hours at 37°C before the plates are washed twice with cold assay buffer. ABTS peroxidase substrate (Biorad) solution is added to the wells and the reaction is stopped after 10 min. Bound sLe'-polymer complex is determined by measuring the optical density at 405 nm in a microplate reader. In this assay, compounds of formula I inhibit the sLe^polymer/E-selectin binding interaction when used at a concentration of from 0.07 to 10 nM. Compounds of Examples 12, 13 and 18 inhibit the SLe'-polymer/E-selectin binding interaction at an IC JO (50 % inhibition) of 1 mM, 10 mM and 0.5 mM, respectively. c) In vivo assays. A compound of formula I may be used as a one-for-one replacement for SLe* in vivo treatments, e.g. as described in the rat/cobra venom model by Mulligan et al., Nature, 2_24* 149-151 (1993) or in the feline model of myocardial ischaemia reperfusion injury by Murohara et al., Cardiovascular Research 2Ω, 965-974 (1995). Compounds of formula I show a beneficial effect in these models. The compounds of formula I are, therefore, useful in the treatment and/or prevention of disorders or diseases which are mediated by the binding of selectins in cellular adhesion, particularly E-selectin, e.g. acute or chronic inflammatory or autoimmune diseases such as rheumatoid arthritis, asthma, allergy conditions, psoriasis, contact dermatitis, adult respiratory distress syndrome, inflammatory bowel disease and ophthalmic inflammatory diseases, infection diseases such as septic shock, traumatic shock, thrombosis and inappropriate platelet aggregation conditions, cardiovascular diseases such as heart attacks, reperfusion injury, multiple sclerosis and neoplastic diseases including metastasis conditions, strokes and acute or chronic rejection of organ or tissue transplants. For the above uses the required dosage will of course vary depending on the mode of administration, the particular condition to be treated and the effect desired. In general, however, satisfactory results are achieved at dosage rates of from about 0.5 to 80 mg/kg animal body weight. Suitable daily dosage rates for larger mammals, for example humans, are of the order of from about 100 mg to 1.5 g/day, conveniently administered once, in divided dosages 2 to 4 x / day, or in sustained release form. Unit dosage forms suitably comprise from about 25 mg to 0.750 g of a compound of formula I, together with a pharmaceutical acceptable diluent or carrier therefor. Compounds of formula I may be administered in free form or in pharmaceutically acceptable salt form. Such salts may be prepared in conventional manner and exhibit the same order of activity as the free compounds. The present invention also provides a pharmaceutical composition comprising a compound of the invention, in free base form or in pharmaceutically acceptable salt form in association with a pharmaceutically acceptable diluent or carrier. Such compositions may be formulated in conventional manner. Compounds of the invention may be administered by any conventional route, for example parenteraily e.g in form of injectable solutions or suspensions, or in a nasal or a suppository form. In accordance with the foregoing the present invention further provides: a) a compound of the invention or a pharmaceutically acceptable salt for use as a pharmaceutical; b) a method for preventing or treating disorders as indicated above in a subject in need of such treatment, which method comprises administering to said subject an effective amount of a compound of formula I or a pharmaceutically acceptable salt; c) a compound of the invention or a pharmaceutically acceptable salt for use in the preparation of a pharmaceutical composition for use in the method as in b) above.

Claims: CLAIMS 1. A compound of formula I wherein i) R is CH 3 , and either R, is a radical of formulae (a,) or (a 2 ) (a,) (^ wherein m is 2 or 3; n is 2 or 3; M is a cation; R 2 is H or a saturated or unsaturated hydrocarbon residue with up to 20 carbon atoms, optionally bearing in ω position a formyl or a C M alcohol acetal or C 2 - 4 diol acetal group; R 3 is H, -CH 2 OH or -CH 2 CH 2 OH; and R 4 is H, C M alkyl, -CH 2 OH, -CH 2 CH 2 OH or -CH 2 CH 2 CH 2 OH with the provisos that 1 ) one of R 3 and R 4 is H, and 2) when R 4 is H, R 3 is -CH 2 OH or -CH 2 CH 2 OH, and 3) when R 3 is H, R 4 is CH 3 , -CH 2 OH, -CH 2 CH 2 OH or -CH 2 CH 2 CH 2 OH; is a radical of formula (b) wherein R 5 is MOOC X CH 2 -r- CH- CO- NH- C — (h.) p I I Re RT (b 2 ) (b 3 ) or MOOC — CH- • CH CO— NH 2 ' r wherein p is 1 or 2; q is 2 or 3; r is 1 or 2; R 6 is H, NH 2 or -NHR X wherein R. is an amino protecting group; R 7 . is -CH 2 OH, -CH 2 CH 2 OH or -CH(OH)-CH 2 OH and R^ is H or each of R 7a and R^ is CH 2 OH; R,, is H or -OH; R 13 is -(CH 2 ) COOM or -SO 3 M wherein j is 1, 2 or 3; and M is as defined above; the second hydroxy substituent of the phenyl group in (b 4 ) being in either meta position; fil R, is a radical of formula (c) I O-CH - CH, I (c) R β - CO - NH - CH - COR β wherein R 8 is OM,, OR, 4 , R s -R p or NHR y wherein M, is a cation, R l4 is a saturated or unsaturated hydrocarbon residue, R s is a spacer group, R p is a phosphatidyl residue and R y is a lipophilic residue; and R, is MOOC - (c,) (c 2 ) (C3) (C ) wherein s is 1 or 2; t is 1 or 2; v is 2 or 3; M, R^, R,, and R 13 are as defined above; and R 10l is -CH 2 OH. -CH 2 CH 2 OH or -CH(OH)-CH 2 OH and R 10b is H or each of R 10a and R^ is CH 2 OH; the second hydroxy substituent of the phenyl group in (c 2 ) being in either meta position; or wherein ii) R, is OH, and R is a radical of formula (d) • 12a I H ■ (d) MOOC-f; CH 2 - - CH - CO- N- C - CO- NH - CH 2 - I R 12b wherein w is 1 or 2; R 12a is -CH(OH)-(CH 2 ) x -OH and R 12b is H or each of R 12i and R l2b independently is -CH 2 OH or -CH 2 CH 2 OH; x is 2 or 3; and R 6 and M are as defined above. 2. A compound of formula I according to claim 1 wherein R is CH 3 and R, is a radical of formula (c), R, being a radical (c,) or (c 3 ). 3. A compound of formula I according to claim 1 wherein R, is OH and R is a radical of formula (d). 4. A compound of formula wherein M is a cation. 5. A compound of formula CH MO j C-r- CH j -) — or OH wherein M is a cation. 6. A compound of formula or wherein M is a cation. 7. A process for the production of a compound of formula I according to claim 1 comprising removing at least one protecting group which is present in a compound of formula I in protected form, and where required, recovering the compounds of formula I thus obtained in free form or in salt form. 8. A compound according to any one of claims 1 to 6 for use as a pharmaceutical. 9. A pharmaceutical composition comprising a compound according to any one of claims 1 to 6, together with a pharmaceutically acceptable diluent or carrier therefor. 10. A method for preventing or treating disorders or diseases which are mediated by the binding of selectins in cellular adhesion in a subject in need of such treatment, which method comprises administering to said subject an effective amount of a compound according to any one of claims 1 to 6.

Inventors: KAJIMOTO TETSUYA, WONG CHI-HUEY, KAJIMOTO, TETSUYA, WONG, CHI-HUEY
Applicants: KAJIMOTO TETSUYA, SANDOZ LTD, SCRIPPS RESEARCH INST, WONG CHI HUEY, KAJIMOTO, TETSUYA, SANDOZ LTD., THE SCRIPPS RESEARCH INSTITUTE, WONG, CHI-HUEY
Application year: 1996
Main classification: C07K9
IPC codes: A61K31, A61K38, A61P7, A61P9, A61P29, A61P31, A61P35, A61P37, C07D309, C07H5, C07H7, C07H15, C07K9