MODULATORS OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR-GAMMA, AND METHODS FOR THE USE THEREOF

Abstract: In accordance with the present invention, there is provided a class of compounds which are capable of modulating processes mediated by peroxisome proliferator activated receptor-gamma (PPAR- gamma ). The identification of such compounds makes it possible to intervene in PPAR- gamma mediated pathways.

Description: Modulators of Peroxisome Proliferator Activated Receptor-gamma. and Methods for the Use Thereof FIELD OF THE INVENTION The present invention relates to methods for the modulation of nuclear receptor mediated processes. In a particular aspect, the present invention relates to the use of a specific class of compounds for the modulation of processes mediated by peroxisome proliferator activated receptor-gamma (PPAR- ) . BACKGROUND OF THE INVENTION Peroxisome proliferators are a structurally diverse group of compounds which, when administered to rodents, elicit dramatic increases in the size and number of hepatic and renal peroxisomes, as well as concomitant increases in the capacity of peroxisomes to metabolize fatty acids via increased expression of the enzymes required for the β-oxidation cycle (Lazarow and Fuji i, Ann . Rev . Cell Biol . 1:489-530 (1985); Va ecq and Draye, Essays Biochem . 24:1115-225 (1989); and Nelali et al., Cancer Res . 48:5316-5324 (1988)). Chemicals included in this group are the fibrate class of hypolipidermic drugs, herbicides, and phthalate plasticizers (Reddy and Lalwani, Crit . Rev . Toxicol . 12:1-58 (1983)) . Peroxisome proliferation can also be elicited by dietary or physiological factors such as a high-fat diet and cold acclimatization. Insight into the mechanism whereby peroxisome proliferators exert their pleiotropic effects was provided by the identification of a member of the nuclear hormone receptor superfamily activated by these chemicals (Isseman and Green, Nature 347-645-650 (1990)). This receptor, termed peroxiεome proliferator activated receptor alpha (PPARα) , was subsequently shown to be activated by a variety of medium and long-chain fatty acids and to stimulate expression of the genes encoding rat acyl-CoA oxidase and hydratase-dehydrogenase (enzymes required for peroxisomal β-oxidation) , as well as rabbit cytochrome P450 4A6, a fatty acid ω-hydroxylase (Gottlicher et al., Proc . Natl . Acad . Sci . USA 89:4653-4657 (1992); Tugwood et al., EMBO J . 11:433-439 (1992); Bardot et al., Biochem . Biophys . Res . Comm . 192:37-45 (1993); Muerhoff et al., J . Biol . Chem . 267:19051-19053 (1992); and Marcus et al., Proc. Natl . Acad . Sci . USA 90(12) :5723-5727 (1993). The above-noted references suggest a physiological role for PPARα in the regulation of lipid metabolism. PPARα activates transcription by binding to DNA sequence elements, termed peroxisome proliferator response elements (PPRE) , as a heterodimer with the retinoid X receptor. The retinoid X receptor is activated by 9-cis retinoic acid (see Kliewer et al.. Nature 358:771- 774 (1992), Gearing et al., Proc . Natl . Acad . Sci . USA 90.1440-1444 (1993), Keller et al., Proc . Natl . Acad . Sci . USA £0:2160-2164 (1993), Heyman et al. , Cell 68:397-406 (1992), and Levin et al., Nature 355:359-361 (1992)). Since the PPARα-RXR complex can be activated by peroxisome proliferators and/or 9-cis retinoic acid, the retinoid and fatty acid signaling pathways are seen to converge in modulating lipid metabolism. Since the discovery of PPARα, additional isoforms of PPAR have been identified, e.g., PPAR?, PPAR and PPARό", which are spatially differentially expressed. Because there are several isoforms of PPAR, it would be desirable to identify compounds which are capable of selectively interacting with only one of the PPAR isoforms. Such compounds would find a wide variety of uses, such as, for example, in the prevention of obesity, for the treatment of diabetes, and the like. BRIEF DESCRIPTION OF THE INVENTION In accordance with the present invention, we have identified a class of compounds which are capable of modulating processes mediated by peroxisome proliferator activated receptor-gamma (PPAR- ) . The identification of such compounds makes possible intervention in PPAR- mediated pathways. BRIEF DESCRIPTION OF THE FIGURE Figure 1 illustrates the relative reporter activity induced by two different compounds when added alone or in combination to a GAL4-PPAR fusion protein. In the figure, BRL 49653 refers to 5-[t4-[2-(methyl-2- pyridinylamino) ethoxy]phenyl] -methyl] -2 , 4 - thiazolidenedione) and LY 171883 refers to 2-hydroxy-3- propyl-4-[6-(tetrazole-5-yl)butoxy]acetophenone. DETAILED DESCRIPTION OF THE INVENTION In accordance with the present invention, there are provided methods for modulating process(es) mediated by peroxisome proliferator activated receptor-gamma (PPAR- ) , said method comprising conducting said process(es) in the presence of at least one antagonist or partial-agonist of PPAR- - Antagonists and partial-agonists of PPAR contemplated for use in the practice of the present invention can be described broadly with reference to the general structure I: wherein: each of X,, X 2 , X 3 , X 4 , X 5 and X 6 is independently selected from carbon, nitrogen, oxygen or sulfur, with the proviso that at least three of the atoms forming the ring are carbon, R, is selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalky1 , substituted arylalkyl , arylalkenyl, substituted arylalkenyl, arylalkynyl, substituted arylalkynyl, poly (alkylene oxide) , substituted poly (alkylene oxide) , poly (alkylene sulfide) , substituted poly(alkylene sulfide) , poly(alkylene amine), substituted poly(alkylene amine), -OR, -SR or -NR 2 , wherein each R is independently selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, arylalkyl, substituted arylalkyl, poly(alkylene oxide) , substituted poly (aIkylene oxide) , poly (alkylene sulfide) , substituted poly(alkylene sulfide) , poly(alkylene amine) or substituted poly(alkylene amine) ; with R 1 having in the range of 2 up to 15 carbon atoms being preferred; R 2 is selected from hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, 5 alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl, substituted 10 arylalkyl, arylalkenyl, substituted arylalkenyl, arylalkynyl, substituted arylalkynyl, oxyalkyl, poly(alkylene oxide) or substituted poly(alkylene oxide); with R 2 having in the range of 1 up to about 15 15 carbon atoms being preferred; R 3 is selected from hydrogen, hydroxy, halogen, alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl or substituted alkynyl; with R 3 having in 20 the range of 0 up to about 6 carbon atoms being preferred; R is selected from hydrogen, formyl, acyl, lower alkyl or substituted lower alkyl; with R 4 having in the range of 0 up to about 4 25 carbon atoms being preferred; R 5 is selected from hydrogen, hydroxy, lower alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl or halogen; 30 with R 5 having in the range of 0 up to about 6 carbon atoms being preferred; and R 6 is selected from hydrogen, hydroxy, lower alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, 35 alkynyl, substituted alkynyl or halogen; with R 6 having in the range of 0 up to about 6 carbon atoms being preferred. Those of skill in the art recognize that the core ring of structure I can be any one of a number of different aromatic or pseudo-aromatic structures, e.g., a benzene ring, a pyridine ring, a pyrazine, an oxazine, and the like. As employed herein, "lower alkyl" refers to straight or branched chain alkyl groups having in the range of about 1 up to 4 carbon atoms; "alkyl" refers to straight or branched chain alkyl groups having in the range of about 1 up to 12 carbon atoms; "substituted alkyl" refers to alkyl groups further bearing one or more substituents such as hydroxy, alkoxy (of a lower alkyl group) , mercapto (of a lower alkyl group), halogen, trifluoromethyl, cyano, nitro, amino, carboxyl, carbamate, sulfonyl, sulfonamide, heteroatom-containing cyclic moieties, substituted heteroato -containing cyclic moieties, and the like. As employed herein, "alkenyl" refers to straight or branched chain hydrocarbyl groups having at least one carbon-carbon double bond, and having in the range of about 2 up to 12 carbon atoms and "substituted alkenyl" refers to alkenyl groups further bearing one or more substituents as set forth above. As employed herein, "alkynyl" refers to straight or branched chain hydrocarbyl groups having at least one carbon-carbon triple bond, and having in the range of about 2 up to 12 carbon atoms, and "substituted alkynyl" refers to alkynyl groups further bearing one or more substituents as set forth above. As employed herein, "aryl" refers to aromatic groups having in the range of 6 up to 14 carbon atoms and "substituted aryl" refers to aryl groups further bearing one or more substituents as set forth above. As employed herein, "alkylaryl" refers to alkyl- substituted aryl groups and "substituted alkylaryl" refers to alkylaryl groups further bearing one or more substituents as set forth above. As employed herein, "alkenylaryl" refers to alkenyl-substituted aryl groups and "substituted alkenylaryl" refers to alkenylaryl groups further bearing one or more substituents as set forth above. As employed herein, "alkynylaryl" refers to alkynyl-substituted aryl groups and "substituted alkynylaryl" refers to alkynylaryl groups further bearing one or more substituents as set forth above. As employed herein, "arylalkyl" refers to aryl- substituted alkyl groups and "substituted arylalkyl" refers to arylalkyl groups further bearing one or more substituents as set forth above. As employed herein, "arylalkenyl" refers to aryl- substituted alkenyl groups and "substituted arylalkenyl" refers to arylalkenyl groups further bearing one or more substituents as set forth above. As employed herein, "arylalkynyl" refers to aryl- substituted alkynyl groups and "substituted arylalkynyl" refers to arylalkynyl groups further bearing one or more substituents as set forth above. As employed herein, "poly(alkylene oxide)" refers to compounds having the general structure: -[(CR' 2 ) x -0] y -H, wherein each R 1 is independently selected from hydrogen or lower alkyl, x falls in the range of 1 up to about 4 and y falls in the range of 2 up to about 8; "substituted poly(alkylene oxide)" refers to poly(alkylene oxide) groups further bearing one or more substituents as set forth above. As employed herein, "poly(alkylene sulfide) " refers to compounds having the general structure: -[(CR' 2 ) χ -S] y -H, wherein R', x and y are as defined above; "substituted poly(alkylene sulfide) " refers to poly(alkylene sulfide) groups further bearing one or more substituents as set forth above. As employed herein, "poly(alkylene amine)" refers to compounds having the general structure: -[(CR' 2 ) χ -N(R')] y -H, wherein R', x and y are as defined above; "substituted poly(alkylene amine)" refers to poly(alkylene amine) groups further bearing one or more substituents as set forth above. As employed herein, "heteroatom-containing cyclic moiety" refers to cyclic (i.e., 5-, 6- or 7-membered ring- containing) groups containing one or more heteroato s (e.g., N, O, S, or the like) as part of the ring structure, and having in the range of 1 up to about 14 carbon atoms; and "substituted heteroatom-containing cyclic moiety" refers to heterocyclic groups further bearing one or more substituents as set forth above. Examples of heteroatom- containing cyclic moieties include furans, thiophenes, pyrroles, pyrazoles, diazoles, triazoles, tetrazoles, dithioles, oxathioleε, oxazoles, isoxazoles, thiazoles, isothiazoles, oxadiazoleε, oxatriazoles, dioxazoles, oxathiazoles, pyrans, pyrones, dioxins, pyridines, pyrimidines, pyrazines, pyridazines, piperazines, diazines, triazines, oxazines, isoxazines, oxathiazines, oxadiazines, morpholines, azepins, oxepins, thiopins, diazepins, benzothiazoles, thiazolidinediones, and the like. As employed herein, "acyl" refers to alkyl- carbonyl species. As employed herein, "halogen" or "halo" refers to fluoro substituents, chloro substituents, bromo substituents or iodo substituents. In a presently preferred aspect of the present invention, "R," of Formula I is selected from: -Y n -(CR"R") m -Z, -Y n -(CR"R") m ,-0-(CR"R") m ,-Z, or -Y n -(CR"R") πι „-N(R' ' ')-(CR"R") m „-Z, wherein: Y is -O- or -S-, n is 0 or 1, each R" is independently selected from hydrogen, lower alkyl, substituted lower alkyl, hydroxy, lower alkoxy, thioalkyl, halogen, trifluoromethyl, cyano, nitro, amino, carboxyl, carbamate, sulfonyl or sulfonamide, R 1 ' ' is selected from hydrogen, lower alkyl or substituted allkyl, m falls in the range of 1 up to 15, each m' falls independently in the range of 1 up to 8, each m" falls independently in the range of 0 up to 12, and Z is εelected from a heteroatom-containing cyclic moiety, a substituted heteroatom-containing cyclic moiety, cyano, nitro, amino, carbamate, -OR a , wherein R ,a is selected from H, alkyl, alkenyl, alkynyl, acyl or aryl; -C(0)R , wherein R is selected from H, alkyl, substituted alkyl, alkoxy, alkylamino, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, aryloxy, arylamino, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl, substituted arylalkyl, arylalkenyl, substituted arylalkenyl, arylalkynyl, substituted arylalkynyl, heterocyclic, substituted heterocyclic or trifluoromethyl; -C0 2 R c , wherein R c is selected from H, alkyl, alkenyl, alkynyl or aryl; -SR a , -S(0)R a , -S(0) 2 R a or -S(0) 2 NHR a , wherein each R a is as defined above, and the like. It is presently preferred that Z be selected from heteroatom-containing cyclic moieties, with polyheteroatom- containing cyclic moieties being especially preferred. Those of skill in the art can readily identify numerous groups which fall within the definition of "heteroatom- containing cyclic moieties", as set forth herein. Especially preferred are polyheteroatom-containing cyclic moieties, e.g., pyrazoles, diazoles, triazoles, tetrazoles, dithioles, oxathioles, oxazoles, isoxazoles, thiazoles, isothiazoles, oxadiazoles, oxatriazoles, dioxazoles, oxathiazoles, pyridazines, piperazines, diazines, triazines, oxazines, isoxazines, oxathiazines, oxadiazines, orpholineε, diazepins, thiazolidinedioneε, and the like. Especially preferred compounds employed in the practice of the present invention are those wherein "R," of Formula I is -Y n -(CH 2 ) χ -Z wherein: Y is -O- or -S-, n is 0 or 1, x falls in the range of 2 up to 12; and Z is a triazole, a tetrazole, an oxadiazole, an oxatriazole, a dioxazole, an oxathiazole, a triazine, an isoxazine, an oxathiazine, an oxadiazine, a thiazolidinedione, and the like. A presently preferred species of R 1 is -0-(CH 2 ) 4 -[tetrazoline] . In another preferred aspect of the present invention, "R 2 " of Formula I is selected from methyl, ethyl, propyl, butyl, methoxy, ethoxy, propoxy, butoxy, and the like. In yet another preferred aspect of the present invention, "R 3 " of Formula I is selected from hydrogen, hydroxy, alkoxy, and the like. In still another preferred aspect of the present invention, "R 4 " of Formula I is selected from formyl, acyl, thiazolidenedione, alkyl-substituted thiazolidenedione, and the like. In a further preferred aspect of the present invention, "R 5 " of Formula I is hydrogen. In a still further preferred aspect of the present invention, "R 6 " of Formula I is hydrogen. In yet another preferred aspect of the present invention, at least one of R 2 , R 3 , R 4 , R 5 and R 6 (in addition to R,) are not hydrogen. It is especially preferred that at least two of R 2 , R 3 , R 4 , R 5 and R 6 (in addition to R.,) are not hydrogen. A plurality of substituents on the ring of structure I is especially preferred when the backbone of R 1 contains no greater than 6 atoms. Presently preferred species contemplated for use in the practice of the present invention include compounds wherein: R, is -0-(CH 2 ) 4 -[tetrazoline] , R 2 is hydrogen or lower alkyl, R 3 is hydroxy or alkoxy, R 4 is acyl or thiazolidenedione; and R 5 and R 6 are each hydrogen, as well as compounds wherein: R 1 is -0-(CH 2 ) -thiazolidenedione, wherein y falls in the range of about 2 up to 8; R 2 is hydrogen or lower alkyl, R 3 is hydroxy or alkoxy, R 4 is acyl or thiazolidenedione; and R 5 and R 6 are each hydrogen. The above-described compounds can be readily prepared using a variety of synthetic methods, as are well known by those of skill in the art. For example, many of the above-described compounds can be prepared chemically or enzymatically. As employed herein, the term "modulate" refers to the ability of a modulator for a member of the steroid/thyroid superfamily to either directly (by binding to the receptor as a ligand) or indirectly (as a precursor for a ligand or an inducer which promotes production of ligand from a precursor) induce expression of gene(s) maintained under hormone expression control, or to repress expression of gene(s) maintained under such control. As employed herein, the phrase "processes mediated by PPARy" refers to biological, physiological, endocrinological, and other bodily processes which are mediated by receptor or receptor combinations which are responsive to the PPAR-^ antagonists and partial-agonists described herein (e.g., cell differentiation to produce lipid-accumulating cells, regulation of insulin-sensitivity and blood glucose levels, especially as related to hypoglycemia/hyperinsulinism (resulting, for example, from abnormal pancreatic beta-cell function, insulin-secreting tumors and/or autoimmune hypoglycemia due to autoantibodies to insulin, the insulin receptor or autoantibodies that are stimulatory to pancreatic beta-cells) , the formation of macrophages which lead to the development of atherosclerotic plaques, and the like) . Modulation of such processes can be accomplished in vitro or in vivo . In vivo modulation can be carried out in a wide range of subjects, such as, for example, humans, rodents, sheep, pigs, cows, and the like. PPAR-^-selective antagonists or partial-agonists contemplated for use in the practice of the present invention can be employed for both in vitro and in vivo applications. For in vivo applications, the invention compounds can be incorporated into a pharmaceutically acceptable formulation for administration. Those of skill in the art can readily determine suitable dosage levels when compounds contemplated for use in the practice of the present invention are so used. In accordance with another embodiment of the present invention, there is provided a method of screening for antagonists of PPAR receptor proteins, said method comprising culturing test cells containing (i) increasing concentrations of at least one compound whose ability to inhibit the transcription activation activity of PPARK agonists is sought to be determined, and (ii) optionally, at least one PPARK agonist, wherein said test cells contain (i) exogenous DNA which expresses intact PPARK or a modified form of PPARK, wherein the modified form of PPARK contains the DNA binding domain of GAL4, and (ii) a PPRE or GAL4 response element, respectively, operatively linked to a reporter gene; and thereafter assaying for evidence of transcription of said reporter gene in said cells as a function of the concentration of said compound in said culture medium, thereby indicating the ability of said compound to inhibit activation of transcription by PPARK agonists. Media employed for such culturing may include agonist for the receptor being tested, or the receptor may be constitutive (i.e., not require the presence of agonist for activation) , or a fixed concentration of agonist can be added to the media employed for such testing. The above-described assays of the present invention have low background and a broad dynamic range. Thus, in accordance with the present invention, compound(s) which fall within the structure of Formula I can readily be tested for the ability to regulate the transcription-activating effects of peroxisome proliferator activated receptor-gamma (PPAR-K) . This can be carried out by assaying for changes in the level of reporter protein present as a result of contacting cells containing the receptor and reporter vector with test compound; wherein the reporter vector comprises: (a) a promoter that is operable in the cell, (b) a hormone response element, and (c) a DNA segment encoding a reporter protein, wherein the reporter protein-encoding DNA segment is operatively linked to the promoter for transcription of the DNA segment, and wherein the hormone response element iε operatively linked to the promoter for activation thereof. Hormone response elements contemplated for use in the practice of the present invention are composed of at leaεt one direct repeat of two or more half εites separated by a spacer of one nucleotide. The spacer nucleotide can be selected from any one of A, C, G or T. Each half site of response elements contemplated for use in the practice of the invention comprises the sequence -RGBNNM-, wherein R is selected from A or G; B is εelected from G, C, or T; each N is independently selected from A, T, C, or G; and M is selected from A or C; with the proviso that at least 4 nucleotides of said -RGBNNM- sequence are identical with the nucleotides at corresponding positions of the sequence -AGGTCA-. Response elements employed in the practice of the present invention can optionally be preceded by N χ , wherein x falls in the range of 0 up to 5. Presently preferred response elements contain at least one copy (with one, two or three copies most common) of the minimal sequence: AGGACA A AGGTCA (SEQ ID NO:4). As noted above, the minimal sequence can optionally be flanked by additional residues, for example, as in the sequence: GGACC AGGACA A AGGTCA CGTTC (SEQ ID NO:5). In a preferred embodiment of the present invention, only the ligand binding domain of PPARK is utilized, in combination with the DNA binding domain of GAL4 protein, for the identification of PPARK ligands or ligand-precursors. Thiε allowε one to avoid possible background signal caused by the potential presence of endogenous PPAR isoformε in the host cells used for the assay. The DNA binding domain of the yeast GAL4 protein comprises at least the first 74 amino acids thereof (see, for example, Keegan et al., Science 231:699-704 (1986)). Preferably, the first 90 or more amino acids of the GAL4 protein will be used, with the first 147 amino acid residues of yeast GAL4 being presently most preferred. The GAL4 fragment employed in the practice of the present invention can be incorporated into any of a number of sites within the PPARK receptor protein. For example, the GAL4 DNA binding domain can be introduced at the amino terminus of the PPARK receptor protein, or the GAL4 DNA binding domain can be substituted for the native DNA binding domain of the PPARK receptor, or the GAL4 DNA binding domain can be introduced at the carboxy terminus of the PPARK receptor protein, or at other positions as can readily be determined by those of skill in the art. Thus, for example, a modified receptor protein can be prepared which consists essentially of amino acid residues 1-147 of GAL4, plus the ligand binding domain of PPARK (i.e., containing the ligand binding domain only of said receptor (i.e., residues 163-475 of SEQ ID N0:1), substantially absent the DNA binding domain and amino terminal domain thereof) . Identification methods according to the present invention involve the use of a functional bioassay system, wherein the modified receptor and a reporter plasmid are cultured in suitable host cells in the presence of test compound. Evidence of transcription (e.g. , expression) of reporter gene is then monitored to determine the presence of an activated receptor-ligand complex. Accordingly, the functional bioassay system utilizes two plasmids: an "expression" plasmid and a "reporter" plasmid. The expression plasmid can be any plasmid which contains and is capable of expresεing DNA encoding the deεired form of PPARK receptor protein (i.e., intact receptor or GAL4 chimeric receptor aε deεcribed hereinabove) , in a suitable host cell. The reporter plasmid can be any plasmid which contains an operative PPRE or GAL4 response element, as appropriate, functionally linked to an operative reporter gene. Exemplary PPREs have been described in detail hereinabove. Exemplary GAL4 responεe elements are those containing the palindromic 17-mer: 5'-CGGAGGACTGTCCTCCG-3' (SEQ ID NO:6), such as, for example, 17MX, as described by Webster et al., in Cell 5_2 . : 169-178 (1988), as well as derivatives thereof. Additional examples of suitable response elements include those described by Hollenberg and Evans in Cell 55:899-906 (1988); or Webster et al. in Cell 5_4:199-207 (1988). Exemplary reporter genes include chloramphenicol transferase (CAT) , luciferase (LUC) , beta-galactosidase (?-gal) , and the like. Exemplary promoters include the simian virus (SV) promoter or modified form thereof (e.g., ΔSV) , the thymidine kinase (TK) promoter, the mammary tumor virus (MTV) promoter or modified form thereof (e.g., ΔMTV) , and the like [see, for example, Mangelsdorf et al., in Nature 345:224-229 (1990), Mangelsdorf et al., in Cell j5 :555-561 (1991), and Berger et al. , in J. Steroid Biochem. Molec. Biol. 1:733-738 (1992)]. The plasmidε pGMCAT, pGHCAT, pTK-GAL p 3-LUC, ΔMTV-GAL p 3-LUC, ΔMTV-GAL 3-CAT, and the like, are examples of reporter plasmids which contain an operative hormone responsive promoter/enhancer element functionally linked to an operative reporter gene, and can therefore be used in the above-described functional bioassay (see Example 2 for details on the preparation of these plasmids) . In pGMCAT, the operative hormone responsive promoter/enhancer element is the MTV LTR; in pGHCAT it is the functional portion of the growth hormone promoter. In both pGMCAT and GHCAT the operative reporter gene is the bacterial gene for chloramphenicol acetyltransferase (CAT) . As used herein in the phrase "operative response element functionally linked to an operative reporter gene", the word "operative" means that the respective DNA sequences (represented by the terms "PPRE," "GAL4 response element" and "reporter gene") are operational, i.e., work for their intended purposeε; the word "functionally" means that after the two segments are linked, upon appropriate activation by a ligand-receptor complex, the reporter gene will be expressed as the result of the fact that the "PPRE" or "GAL4 response element" was "turned on" or otherwise activated. In practicing the above-described functional bioassay, the expression plasmid and the reporter plasmid are co-transfected into suitable host cells. The transfected host cells are then cultured in the presence and absence of a test compound to determine if the test compound is able to produce activation of the promoter operatively linked to the PPRE or GAL4 response element of the reporter plasmid. Thereafter, the transfected and cultured host cells are monitored for induction (i.e., the presence) of the product of the reporter gene sequence. Any cell line can be used aε a suitable "host" for the functional bioassay contemplated for use in the practice of the present invention. Thus, in contrast to the requirements of prior art assay systems, when GAL4 chimerics are employed, there iε no need to use receptor- negative cells in carrying out the invention process. Since the modified receptor employed in the practice of the present invention is the only specieε in the teεt cell which iε capable of initiating tranεcription from a GAL4 reεponse element, the expression of native receptor by the test cell does not contribute to background levels. Thus, the invention bioassay can be made to be very selective. Cells contemplated for use in the practice of the present invention include transformed cells, non- transformed cells, neoplastic cells, primary cultures of different cell types, and the like. Exemplary cells which can be employed in the practice of the present invention include Schneider cells, CV-1 cells, HuTuδO cells, F9 cells, NTERA2 cells, NB4 cells, HL-60 cells, 293 cells, Hela cells, yeast cells, and the like. Preferred host cells for use in the functional bioassay system are COS cells and CV-1 cells. COS-l (referred to as COS) cells are monkey kidney cells that expreεs SV40 T antigen (Tag) ; while CV-1 cells do not express SV40 Tag. The presence of Tag in the COS-l derivative lines allows the introduced expression plasmid to replicate and provides a relative increase in the amount of receptor produced during the assay period. CV-1 cells are presently preferred because they are particularly convenient for gene transfer studies and provide a sensitive and well-described host cell system. The above-described cells (or fractions thereof) are maintained under physiological conditions when contacted with physiologically active compound. "Physiological conditionε" are readily understood by those of skill in the art to comprise an isotonic, aqueous nutrient medium at a temperature of about 37°C. In accordance with yet another embodiment of the present invention, there iε provided a method for treating obesity, said method comprising administering to a subject in need thereof an amount of a peroxisome proliferator activated receptor-gamma (PPAR-K) antagonist effective to block cell differentiation to produce lipid-accumulating cells. As employed herein "treating" obeεity embraceε preventing aε well as reversing obesity. As employed here, "obesity" refers generally to individuals who are at leaεt about 20-30% over the average weight for hiε/her age, sex and height. Technically, "obese" is defined, for males, as individuals whose body 2 mass index is greater than 27.8 kg/m , and for females, as individuals whose body mass index is greater than 27.3 kg/m 2 . Those of skill in the art readily recognize that the invention method is not limited to those who fall within the above criteria. Indeed, the invention method can also be advantageously practiced by individuals who fall outside of these traditional criteria, for example, by those who may be prone to obesity. Those of skill in the art recognize that there are numerous cell types which are capable of differentiation to produce "lipid-accumulating cells," such as, for example, mesenchymal cells (e.g., fibroblasts). As employed herein, the phrase "amount... effective to block cell differentiation" refers to levels of compound sufficient to provide circulating concentrations high enough to effect activation of PPARK. Such a concentration typically falls in the range of about 10 nM up to 2 μM; with concentrations in the range of about 100 nM up to 500 nM being preferred. Since the activity of different compounds which fall within the definition of structure I as set forth above may vary considerably, and since individual subjectε may preεent a wide variation in severity of symptomε, it iε up to the practitioner to determine a εubject's response to treatment and vary the dosages accordingly. In accordance with a particular embodiment of the present invention, compositions comprising at least one antagonist or partial-agonist of PPAR-K (as described herein) , and a pharmaceutically acceptable carrier are contemplated. Exemplary pharmaceutically acceptable carriers include carriers suitable for oral, intravenous, subcutaneous, intramuscular, intracutaneous, and the like administration. Administration in the form of creams, lotions, tablets, disperεible powderε, granules, syrups, elixirs, sterile aqueous or non-aqueous solutions, suspensions or emulsions, and the like, is contemplated. For the preparation of oral liquids, suitable carriers include emulsions, solutions, suspensions, syrups, and the like, optionally containing additives such as wetting agents, emulsifying and suspending agents, sweetening, flavoring and perfuming agents, and the like. For the preparation of fluids for parenteral administration, suitable carriers include sterile aqueous or non-aqueous solutions, suspenεions, or emulsions. Examples of non-aqueous solventε or vehicleε are propylene glycol, polyethylene glycol, vegetable oils, such as olive oil and corn oil, gelatin, and injectable organic eεters such as ethyl oleate. Such dosage forms may also contain adjuvants such as preserving, wetting, emulsifying, and dispersing agents. They may be εterilized, for example, by filtration through a bacteria-retaining filter, by incorporating sterilizing agents into the compositions, by irradiating the compositions, or by heating the compositions. They can also be manufactured in the form of sterile water, or some other sterile injectable medium immediately before use. In accordance with still another embodiment of the present invention, there is provided a method for modulating insulin-εensitivity and blood glucose levels in a subject, said method comprising administering to a subject in need of such treatment an amount of a peroxisome proliferator activated receptor-gamma (PPAR~κ) antagonist or partial-agonist effective to lower the blood glucose level of said subject. As employed herein, the phrase "amount... effective to lower blood glucose levels" refers to levels of compound sufficient to provide circulating concentrations high enough to accomplish the desired effect. Such a concentration typically falls in the range of about 10 nM up to 2 μM; with concentrations in the range of about 100 nM up to 500 nM being preferred. As noted previously, since the activity of different compounds which fall within the definition of structure I as set forth above may vary considerably, and since individual subjects may present a wide variation in severity of symptoms, it is up to the practitioner to determine a subject's response to treatment and vary the dosages accordingly. The invention will now be described in greater detail by reference to the following non-limiting examples. Example 1 Preparation of GAL4-receptor fusion proteins A basic vector uεeful for the generation of GAL4- receptor fusion proteins is called pCMX-GAL4 (see SEQ ID N0:2) . This vector encodes GAL4 DNA binding domain, followed by a polylinker sequence useful in the cloning. The parental expreεεion vector pCMX haε been described by Umesono et al., in Cell 5:1255-1266 (1991) , and the GAL4 portion of pCMX-GAL4 iε derived from plaεmid pSG424, deεcribed by Sadowεki and Ptaεhne, in Nucleic Acidε Res. In general, GAL4-receptor ligand binding domain fusions are prepared by taking advantage of mutant receptor cDNA cloneε, εuch aε GR-RAR chimera [see, for example, Giguere et al., in Nature 330:624-629 (1987)]. These mutant receptor cDNAs encode common Xhol sites at the end of the DNA binding domain, as described by Giguere et al., supra♦ To do so, a new pCMX-GAL4 vector was prepared which encodes a compatible Sail site in the polylinker sequence (there is an Xhol εite in the GAL4 sequence) : Sail site: G'TCGAC Xhol εite: C'TCGAG Thiε allowε efficient tranεfer of the receptor ligand binding domain to GAL4 DNA binding domain. Through thiε method, a number of chimeric species have been generated, including GAL4-PPARK, containing residues 163-475 of PPARK (see SEQ ID NO:l) . If mutants of the type referred to above are not available for the construction of GAL4-containing chi erics, one may εimply look for any convenient restriction enzyme site within or downstream of the DNA binding domain of the receptor of interest (i.e., within about the first 30 amino acid residueε downstream of the conserved Gly-Met residues of the DNA binding domain, i.e., within 30 residueε of the laεt two residues shown in SEQ ID N0:1), and utilize the carboxy terminal sequences therefrom. Example 2 Preparation of reporter constructs Various reporter constructs are used in the examples which follow. They are prepared as follows: TK-LUC: The MTV-LTR promoter sequence was removed from the MTV-LUC plasmid described by Hollenberg and Evanε in Cell 55:899-906 (1988) by Hindlll and Xhol digest, and cloned with the HindiII-Xhol fragment of the Herpes simplex virus thymidine kinase gene promoter (-105 to +51 with respect to the transcription εtart εite, m, iεolated from plasmid pBLCAT2, described by Luckow & Schutz in Nucleic Acids Res. JL5_:5490 (1987)) to generate parental construct TK-LUC. pTK-PPRE3-LUC: Three copies of double-stranded peroxisome proliferator response element (PPRE) oligonucleotides (see SEQ ID NO:5) were cloned upstream of the TK promoter of TK-LUC at the Sail site. pTK-MHl00x4-LUC: Four copies of double-stranded MH100 oligonucleotides, encoding a GAL4 binding εite, were cloned upstream of the TK promoter of TK-LUC at the Hindlll site. CMX-yffGAL: The coding sequence for the E . coli ?-galactosidaεe gene waε iεolated from plasmid pCHHO [see Hall et al., J. Mol. Appl. Genet. 2..-101-109 (1983)] by Hindlll and BamHI digest, and cloned into pCMX eucaryotic expression vector [see Umesono et al., εupra] . Example 3 Screening assay for PPAR-K antaqoniεts Effector plasmid, reporter plasmid, and / ff-galactosidase control plasmid are co-transfected into CV-1 cells at a ratio of about 1:3:5, using a liposome- mediated method, employing N-{2-(2, 3) -dioleoyloxy)propyl- N,N,N-trimethyl ammonium methyl εulfate} (i.e., DOTAP, Boehringer Mannheim) according to the manufacturer's instructions in Dulbecco's modified Eagle's medium (DMEM) with 10% delipidated hormone-depleted fetal calf serum. After about 2-3 hours, the cells are waεhed with DMEM and agonist (200 nM BRL 49653) and/or an appropriate test compound (LY 171883; see Figure 1) is added to the media. After 24-48 hourε of incubation, the cellε are rinsed with phosphate buffered saline (pH 7.2) and lysed. Aliquots are asεayed for luciferase and ?-galactosidase activity. Luciferase activity is normalized to optical density units of yff-galactosidase per minute of incubation. Thus, CV-1 cellε are co-tranεfected with CMX-GAL-PPARK and pTK-MH100x4-LUC at a ratio of about 100 ng of receptor-encoding DNA per 10 cellε. The usual amounts of DNA per 10 cells are 100 ng of CMX-GAL-PPARK, 300 ng of pTK-MH100x4-LUC, and 500 ng of CMX-βGAL. Typically, transfections are performed in triplicate. The plates are then incubated for 2-3 hours at 37°C. The cells are washed with fresh medium. Fresh medium containing agonist (200 nM BRL 49653) and/or an appropriate test compound (LY 171883; see Figure 1) is added to the media. A solvent control is also performed. The cells are incubated at 37°C for 1-2 days. The cells are rinsed twice with buffered saline solution. Subsequently, cells are lysed, in situ , by adding 200 μl of lysis buffer. After 30 minutes incubation at room temperature, 40 μl aliquots of cell lysate are transferred to 96-well plateε for luciferase reporter gene assays and ?-galactosidase transfection controls [see Heyman et al., Cell 6>8 . :397-406 (1992)]. The data are expresεed aε relative light units (RLUs) per O.D. unit of /?-galactoεidase per minute. The triplicates are averaged and plotted (see Figure 1) as relative reporter activity induced by agonist alone, antagonist alone, or combinations thereof. Review of the data presented in Figure 1 reveals that 2-hydroxy-3-propyl- 4-[6-(tetrazole-5-yl)butoxy]acetophenone (i.e., LY 171883) is a functional antagonist of PPARK- While the invention has been described in detail with reference to certain preferred embodiments thereof, it will be understood that modifications and variations are within the spirit and scope of that which is described and claimed. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Evans, Ronald M. Forman, Barry M. (ii) TITLE OF INVENTION: MODULATORS OF PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR-GAMMA, AND METHODS FOR THE USE THEREOF (iii) NUMBER OF SEQUENCES: 6 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Pretty, Schroeder, Brueggemann & Clark (B) STREET: 444 South Flower Street, Suite 2000 (C) CITY: Los Angeles (D) STATE: CA (E) COUNTRY: USA (F) ZIP: 90071 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: US 08/477,493 (B) FILING DATE: 07-JUN-1995 (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Reiter, Stephen E. (B) REGISTRATION NUMBER: 31,192 (C) REFERENCE/DOCKET NUMBER: P41 9958 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 619-546-1995 (B) TELEFAX: 619-546-9392 (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2005 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: both (ii) MOLECULE TYPE: cDNA (i ) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 352..1776 (xi) SEQUENCE DESCRIPTION: SEQ ID Nθ:l: ATCGAATCCC GCGCCCCAGG CGCTGCCGCT CTGAGTGCGA CGGGCCCCGC CTGGCCGGCC 60 GGAGGACGCG GAAGAAGAGA CCTGGGGCGC TGCCTGGGGT ATTGGGTCGC GCGCAGTGAG 120 GGGACCGAGT GTGACGACAA GGTGACCGGG CTGAGGGGAC GGGCTGAGGA GAAGTCACAC 180 TCTGACAGGA GCCTGTGAGA CCAACAGCCT GACGGGGTCT CGGTTGAGGG GACGCGGGCT 240 GAGAAGTCAC GTTCTGACAG GACTGTGTGA CAGACAAGAT TTGAAAGAAG CGGTGAACCA 300 CTGATATTCA GGACATTTTT AAAAACAAGA CTACCCTTTA CTGAAATTAC C ATG GTT 357 Met Val 1 GAC ACA GAG ATG CCA TTC TGG CCC ACC AAC TTC GGA ATC AGC TCT GTG 405 Asp Thr Glu Met Pro Phe Trp Pro Thr Asn Phe Gly lie Ser Ser Val 5 10 15 GAC CTC TCC GTG ATG GAA GAC CAC TCG CAT TCC TTT GAC ATC AAG CCC 453 Asp Leu Ser Val Met Glu Asp His Ser His Ser Phe Asp lie Lys Pro 20 25 30 TTT ACC ACA GTT GAT TTC TCC AGC ATT TCT GCT CCA CAC TAT GAA GAC 501 Phe Thr Thr Val Asp Phe Ser Ser lie Ser Ala Pro His Tyr Glu Asp 35 40 45 50 ATT CCA TTC ACA AGA GCT GAC CCA ATG GTT GCT GAT TAC AAA TAT GAC 549 lie Pro Phe Thr Arg Ala Asp Pro Met Val Ala Asp Tyr Lys Tyr Asp 55 60 65 CTG AAG CTC CAA GAA TAC CAA AGT GCG ATC AAA GTA GAA CCT GCA TCT 597 Leu Lys Leu Gin Glu Tyr Gin Ser Ala lie Lys Val Glu Pro Ala Ser 70 75 80 CCA CCT TAT TAT TCT GAA AAG ACC CAG CTC TAC AAC AGG CCT CAT GAA 645 Pro Pro Tyr Tyr Ser Glu Lys Thr Gin Leu Tyr Asn Arg Pro His Glu 85 90 95 GAA CCT TCT AAC TCC CTC ATG GCC ATT GAG TGC CGA GTC TGT GGG GAT 693 Glu Pro Ser Asn Ser Leu Met Ala lie Glu Cys Arg Val Cys Gly Asp 100 105 110 AAA GCA TCA GGC TTC CAC TAT GGA GTT CAT GCT TGT GAA GGA TGC AAG 741 Lys Ala Ser Gly Phe His Tyr Gly Val His Ala Cys Glu Gly Cys Lys 115 120 125 130 GGT TTT TTC CGA AGA ACC ATC CGA TTG AAG CTT ATT TAT GAT AGG TGT 789 Gly Phe Phe Arg Arg Thr lie Arg Leu Lys Leu lie Tyr Asp Arg Cys 135 140 145 GAT CTT AAC TGC CGG ATC CAC AAA AAA AGT AGA AAT AAA TGT CAG TAC 837 Asp Leu Asn Cys Arg lie His Lys Lys Ser Arg Asn Lys Cys Gin Tyr 150 155 160 TGT CGG TTT CAG AAG TGC CTT GCT GTG GGG ATG TCT CAC AAT GCC ATC 885 Cys Arg Phe Gin Lys Cys Leu Ala Val Gly Met Ser His Asn Ala lie 165 170 175 AGG TTT GGG CGG ATG CCA CAG GCC GAG AAG GAG AAG CTG TTG GCG GAG 933 Arg Phe Gly Arg Met Pro Gin Ala Glu Lys Glu Lys Leu Leu Ala Glu 180 185 190 ATC TCC AGT GAT ATC GAC CAG CTG AAC CCA GAG TCT GCT GAT CTG CGA 981 lie Ser Ser Asp lie Asp Gin Leu Asn Pro Glu Ser Ala Asp Leu Arg 195 200 205 210 GCC CTG GCA AAG CAT TTG TAT GAC TCA TAC ATA AAG TCC TTC CCG CTG 1029 Ala Leu Ala Lys His Leu Tyr Asp Ser Tyr lie Lys Ser Phe Pro Leu 215 220 225 ACC AAA GCC AAG GCG AGG GCG ATC TTG ACA GGA AAG ACA ACG GAC AAA 1077 Thr Lys Ala Lys Ala Arg Ala lie Leu Thr Gly Lys Thr Thr Asp Lys 230 235 240 TCA CCA TTT GTC ATC TAC GAC ATG AAT TCC TTA ATG ATG GGA GAA GAT 1125 Ser Pro Phe Val lie Tyr Asp Met Asn Ser Leu Met Met Gly Glu Asp 245 250 255 AAA ATC AAG TTC AAA CAT ATC ACC CCC CTG CAG GAG CAG AGC AAA GAG 1173 Lys lie Lys Phe Lys His lie Thr Pro Leu Gin Glu Gin Ser Lys Glu 260 265 270 GTG GCC ATC CGA ATT TTT CAA GGG TGC CAG TTT CGA TCC GTA GAA GCC 1221 Val Ala lie Arg lie Phe Gin Gly Cys Gin Phe Arg Ser Val Glu Ala 275 280 285 290 GTG CAA GAG ATC ACA GAG TAT GCC AAA AAT ATC CCT GGT TTC ATT AAC 1269 Val Gin Glu lie Thr Glu Tyr Ala Lys Asn lie Pro Gly Phe lie Asn 295 300 305 CTT GAT TTG AAT GAC CAA GTG ACT CTG CTC AAG TAT GGT GTC CAT GAG 1317 Leu Asp Leu Asn Asp Gin Val Thr Leu Leu Lys Tyr Gly Val His Glu 310 315 320 ATC ATC TAC ACG ATG CTG GCC TCC CTG ATG AAT AAA GAT GGA GTC CTC 1365 He He Tyr Thr Met Leu Ala Ser Leu Met Asn Lys Asp Gly Val Leu 325 330 335 ATC TCA GAG GGC CAA GGA TTC ATG ACC AGG GAG TTC CTC AAA AGC CTG 1413 He Ser Glu Gly Gin Gly Phe Met Thr Arg Glu Phe Leu Lys Ser Leu 340 345 350 CGG AAG CCC TTT GGT GAC TTT ATG GAG CCT AAG TTT GAG TTT GCT GTG 1461 Arg Lys Pro Phe Gly Asp Phe Met Glu Pro Lys Phe Glu Phe Ala Val 355 360 365 370 AAG TTC AAT GCA CTG GAA TTA GAT GAC AGT GAC TTG GCT ATA TTT ATA 1509 Lys Phe Asn Ala Leu Glu Leu Asp Asp Ser Asp Leu Ala He Phe He 375 380 385 GCT GTC ATT ATT CTC AGT GGA GAC CGC CCA GGC TTG CTG AAC GTG AAG 1557 Ala Val He He Leu Ser Gly Asp Arg Pro Gly Leu Leu Asn Val Lys 390 395 400 CCC ATC GAG GAC ATC CAA GAC AAC CTG CTG CAG GCC CTG GAA CTG CAG 1605 Pro He Glu Asp He Gin Asp Asn Leu Leu Gin Ala Leu Glu Leu Gin 405 410 415 CTC AAG CTG AAT CAC CCA GAG TCC TCT CAG CTG TTC GCC AAG GTG CTC 1653 Leu Lys Leu Asn His Pro Glu Ser Ser Gin Leu Phe Ala Lys Val Leu 420 425 430 CAG AAG ATG ACA GAC CTC AGG CAG ATC GTC ACA GAG CAC GTG CAG CTA 1701 Gin Lys Met Thr Asp Leu Arg Gin He Val Thr Glu His Val Gin Leu 435 440 445 450 CTG CAT GTG ATC AAG AAG ACA GAG ACA GAC ATG AGC CTT CAC CCC CTG 1749 Leu His Val He Lys Lys Thr Glu Thr Asp Met Ser Leu His Pro Leu 455 460 465 CTC CAG GAG ATC TAC AAG GAC TTG TAT TAGCAGGAAA GTCCCACCCG 1796 Leu Gin Glu He Tyr Lys Asp Leu Tyr 470 475 CTGACAACGT GTTCCTTCTA TTGATTGCAC TATTATTTTG AGGGAAAAAA ATCTGACACC 1856 TAAGAAATTT ACTGTGAAAA AGCATTTAAA AACAAAAAGT TTTAGAACAT GATCTATTTT 1916 ATGCATATTG TTTATAAAGA TACATTTACA ATTTACTTTT AATATTAAAA ATTACCACAT 1976 TATAAAAAAA AAAAAAAAAA AGGAATTCC 2005 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 546 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: both (ii) MOLECULE TYPE: cDNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 35.-544 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: GGGAGACCCA AGCTTGAAGC AAGCCTCCTG AAAG ATG AAG CTA CTG TCT TCT 52 Met Lys Leu Leu Ser Ser 1 5 ATC GAA CAA GCA TGC GAT ATT TGC CGA CTT AAA AAG CTC AAG TGC TCC 100 He Glu Gin Ala Cys Asp He Cys Arg Leu Lys Lys Leu Lys Cys Ser 10 15 20 AAA GAA AAA CCG AAG TGC GCC AAG TGT CTG AAG AAC AAC TGG GAG TGT 148 Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu Lys Asn Asn Trp Glu Cys 25 30 35 CGC TAC TCT CCC AAA ACC AAA AGG TCT CCG CTG ACT AGG GCA CAT CTG 196 Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro Leu Thr Arg Ala His Leu 40 45 50 ACA GAA GTG GAA TCA AGG CTA GAA AGA CTG GAA CAG CTA TTT CTA CTG 244 Thr Glu Val Glu Ser Arg Leu Glu Arg Leu Glu Gin Leu Phe Leu Leu 55 60 65 70 ATT TTT CCT CGA GAA GAC CTT GAC ATG ATT TTG AAA ATG GAT TCT TTA 292 He Phe Pro Arg Glu Asp Leu Asp Met He Leu Lys Met Asp Ser Leu 75 80 85 CAG GAT ATA AAA GCA TTG TTA ACA GGA TTA TTT GTA CAA GAT AAT GTG 340 Gin Asp He Lys Ala Leu Leu Thr Gly Leu Phe Val Gin Asp Asn Val 90 95 100 AAT AAA GAT GCC GTC ACA GAT AGA TTG GCT TCA GTG GAG ACT GAT ATG 388 Asn Lys Asp Ala Val Thr Asp Arg Leu Ala Ser Val Glu Thr Asp Met 105 110 115 CCT CTA ACA TTG AGA CAG CAT AGA ATA AGT GCG ACA TCA TCA TCG GAA 436 Pro Leu Thr Leu Arg Gin His Arg He Ser Ala Thr Ser Ser Ser Glu 120 125 130 GAG AGT AGT AAC AAA GGT CAA AGA CAG TTG ACT GTA TCG CCG GAA TTC 484 Glu Ser Ser Asn Lys Gly Gin Arg Gin Leu Thr Val Ser Pro Glu Phe 135 140 145 150 CCG GGG ATC CGT CGA CGG TAC CAG ATA TCA GGA TCC TGG CCA GCT AGC 532 Pro Gly He Arg Arg Arg Tyr Gin He Ser Gly Ser Trp Pro Ala Ser 155 160 165 TAG GTA GCT AGA GG 546 * Val Ala Arg 170 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 170 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: Met Lys Leu Leu Ser Ser He Glu Gin Ala Cys Asp He Cys Arg Leu 1 5 10 15 Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys Ala Lys Cys Leu 20 25 30 Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr Lys Arg Ser Pro 35 40 45 Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg Leu Glu Arg Leu 50 55 60 Glu Gin Leu Phe Leu Leu He Phe Pro Arg Glu Asp Leu Asp Met He 65 70 75 80 Leu Lys Met Asp Ser Leu Gin Asp He Lys Ala Leu Leu Thr Gly Leu 85 90 95 Phe Val Gin Asp Asn Val Asn Lys Asp Ala Val Thr Asp Arg Leu Ala 100 105 110 Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gin His Arg He Ser 115 120 125 Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly Gin Arg Gin Leu 130 135 140 Thr Val Ser Pro Glu Phe Pro Gly He Arg Arg Arg Tyr Gin He Ser 145 150 155 160 Gly Ser Trp Pro Ala Ser * Val Ala Arg 165 170 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: both (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: AGGACAAAGG TCA 13 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: both (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5; GGACCAGGAC AAAGGTCACG TTC 23 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: both (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: CGGAGGACTG TCCTCCG 17

Claims: That which is claimed is: 1. A method for modulating process(es) mediated by peroxisome proliferator activated receptor-gamma (PPAR-K) , said method comprising conducting said process (es) in the presence of at least one antagonist or partial-agonist of PPAR-K- 2. A method according to Claim 1, wherein said antagonist or partial-agonist of PPAR-K has the structure wherein: each of X 1 , X 2 , X 3 , X 4 , X 5 and X 6 is independently selected from carbon, nitrogen, oxygen or sulfur, with the proviso that at least three of the atoms forming the ring are carbon, R 1 is selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl , substituted arylalkyl , arylalkenyl, substituted arylalkenyl, arylalkynyl, substituted arylalkynyl, poly (alkylene oxide) , substituted poly (alkylene oxide) , poly(alkylene sulfide) , substituted poly (alkylene 30 εulfide) , poly(alkylene amine), substituted poly(alkylene amine), -OR, -SR, -NR 2 , wherein each R is independently selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted 35 alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, arylalkyl, substituted arylalkyl, poly(alkylene oxide) , substituted poly (alkylene oxide) , poly(alkylene sulfide) , substituted 40 poly(alkylene sulfide) , poly(alkylene amine) or substituted poly(alkylene amine); R 2 is selected from hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, 45 substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl, subεtituted arylalkyl, arylalkenyl, substituted 50 arylalkenyl, arylalkynyl, substituted arylalkynyl, oxyalkyl, poly(alkylene oxide) or substituted poly(alkylene oxide) ; R 3 iε εelected from hydrogen, hydroxy, halogen, alkoxy, lower alkyl, εubεtituted lower 55 alkyl, alkenyl, εubεtituted alkenyl, alkynyl or εubstituted alkynyl; R 4 is selected from hydrogen, formyl, acyl, lower alkyl or εubεtituted lower alkyl; R 5 is selected from hydrogen, hydroxy, lower 60 alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl or halogen; and R 6 is selected from hydrogen, hydroxy, lower alkoxy, lower alkyl, εubstituted lower 65 alkyl, alkenyl, εubstituted alkenyl, alkynyl, substituted alkynyl or halogen. 3. A method according to claim 2 wherein R 1 of Formula I is selected from: -Y n -(CR"R") m -Z, -Y n -(CR"R") n ,-O-C(R"R"),.,,-Z, or -Y n -(CR"R") m ,-N(R' ")-CR"R") m „-Z, wherein: Y iε -O- or -S-, n iε 0 or 1, each R" iε independently selected from hydrogen, lower alkyl, substituted lower alkyl, hydroxy, lower alkoxy, thioalkyl, halogen, trifluoromethyl, cyano, nitro, amino, carboxyl, carbamate, sulfonyl or sulfonamide, R' ' ' is selected from hydrogen, lower alkyl or εubεtituted alkyl, m falls in the range of 1 up to 15, each m' falls independently in the range of 1 up to 8, each m" falls independently in the range of 0 up to 12, and Z is a heteroatom-containing cyclic moiety, a substituted heteroatom-containing cyclic moiety, cyano, nitro, amino, carbamate, -0R a , wherein R a is selected from H, alkyl, alkenyl, alkynyl, acyl or aryl; -C(0)R , wherein R is selected from H, alkyl, substituted alkyl, alkoxy, alkylamino, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, εubstituted aryl, aryloxy, arylamino, alkylaryl, εubstituted alkylaryl, arylalkyl, substituted arylalkyl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl, substituted arylalkyl, arylalkenyl, subεtituted arylalkenyl, arylalkynyl, εubstituted arylalkynyl, heterocyclic, substituted heterocyclic or trifluoromethyl; -C0 2 R c , wherein R c is selected from H, alkyl, alkenyl or alkynyl; -SR a , -S(0)R a , -S(0) 2 R a or -S(0) 2 NHR a , wherein each R a is as defined above. 4. A method according to claim 3 wherein Z is a heteroatom-containing cyclic moiety or a substituted heteroatom-containing cyclic moiety. 5. A method according to claim 3 wherein Z is a polyheteroatom-containmg cyclic moiety or a substituted polyheteroato -containing cyclic moiety. 6. A method according to claim 3 wherein Z is a furan, thiophene, pyrrole, pyrazole, diazole, triazole, tetrazole, dithiole, oxathiole, oxazole, isoxazole, thiazole, iεothiazole, oxadiazole, oxatriazole, dioxazole, oxathiazole, pyran, pyrone, dioxin, pyridine, pyrimidine, pyrazine, pyridazine, piperazine, diazine, triazine, oxazine, isoxazine, oxathiazine, oxadiazine, morpholino, azepin, oxepin, thiopin, diazepin, benzothiazole or a thiazolidinedione. 7. A method according to claim 3 wherein Z is a pyrazole, diazole, triazole, tetrazole, dithiole, oxathiole, oxazole, isoxazole, thiazole, isothiazole, oxadiazole, oxatriazole, dioxazole, oxathiazole, pyridazine, piperazine, diazine, triazine, oxazine, isoxazine, oxathiazine, oxadiazine, morpholine, diazepin or a thiazolidinedione. 8. A method according to claim 3 wherein R^ of Formula I has the structure: -Y n -(CH 2 ) χ -Z wherein: Y is -O- or -S-, n is 0 or 1, x falls in the range of 2 up to 12; and Z is a triazole, tetrazole, oxadiazole, oxatriazole, dioxazole, oxathiazole, triazine, isoxazine, oxathiazine, oxadiazine or a thiazolidinedione. 9. A method according to claim 2 wherein R., iε -0-(CH 2 ) 4 -[tetrazoline] . 10. A method according to claim 2 wherein R 2 of Formula I iε εelected from methyl, ethyl, propyl, butyl, methoxy, ethoxy, propoxy or butoxy. 11. A method according to claim 2 wherein R 3 of Formula I iε selected from hydrogen, hydroxy or alkoxy. 12. A method according to claim 2 wherein R 4 of Formula I is selected from formyl, acyl, thiazolidenedioneε or alkyl-εubεtituted thiazolidenedioneε. 13. A method according to claim 2 wherein R 5 of Formula I is hydrogen. 14. A method according to claim 2 wherein R 6 of Formula I is hydrogen. 15. A method according to claim 1, wherein at least one of R 2 , R 3 , R 4 , R 5 and R 6 are not hydrogen. 16. A method according to claim 1, wherein at least two of R 2 , R 3 , R 4 , R 5 and R 6 are not hydrogen. 17. A method according to claim 16 wherein the backbone of R 1 contains no greater than 6 atoms. 18. A method according to claim 2 wherein: R 1 is -0-(CH 2 ) 4 -[tetrazoline] , R 2 is hydrogen or lower alkyl, R 3 is hydroxy or alkoxy, R 4 is acyl or thiazolidenedione; and R 5 and R 6 are each hydrogen. 19. A method according to claim 2 wherein: R 1 is -0-(CH 2 ) -thiazolidenedione, wherein y falls in the range of about 2 up to 8; R 2 is hydrogen or lower alkyl, R 3 iε hydroxy or alkoxy, R 4 is acyl or thiazolidenedione; and R 5 and R 6 are each hydrogen. 20. A method according to claim 1 wherein said procesε mediated by PPAR-K iε cell differentiation to produce lipid-accu ulating cells. 21. A method according to claim 1 wherein the process(es) mediated by PPAR-K are insulin-εensitivity and blood glucose levels of the recipient. 22. A method for treating obesity, said method comprising administering to a subject in need of such treatment an amount of a peroxisome proliferator activated receptor-gamma (PPAR-K) antagonist or partial-agonist effective to block cell differentiation to produce lipid- accumulating cells. 23. A method according to Claim 22, wherein said antagonist or partial-agonist of PPAR-K has the structure wherein: each of X 1 , X 2 , X 3 , X 4 , X 5 and X 6 iε independently selected from carbon, nitrogen, oxygen or sulfur, with the proviso that at least three of the atoms forming the ring are carbon, R, is selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl , substituted alkynylaryl, arylalkyl , substituted arylalkyl , arylalkenyl , substituted arylalkenyl, arylalkynyl, subεtituted arylalkynyl, poly ( alkylene oxide ) , substituted poly (alkylene oxide) , poly (alkylene sulf ide) , subεtituted poly (alkylene sulf ide) , poly (alkylene amine) , substituted poly (alkylene amine) , -OR, -SR, -NR 2 , wherein each R is independently εelected from alkyl, εubεtituted alkyl, alkenyl, substituted alkenyl, alkynyl, subεtituted alkynyl, aryl, εubεtituted aryl, alkylaryl, substituted alkylaryl, arylalkyl, substituted arylalkyl, poly (alkylene oxide) , substituted poly (alkylene oxide) , poly(alkylene sulfide) , substituted poly(alkylene sulfide) , poly(alkylene amine) or substituted poly(alkylene amine); R 2 is selected from hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl, substituted arylalkyl, arylalkenyl, subεtituted arylalkenyl, arylalkynyl, substituted arylalkynyl, oxyalkyl, poly(alkylene oxide) or substituted poly(alkylene oxide) ; R 3 is εelected from hydrogen, hydroxy, halogen, alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl or εubεtituted alkynyl; R 4 iε εelected from hydrogen, formyl, acyl, lower alkyl or substituted lower alkyl; R 5 is selected from hydrogen, hydroxy, lower alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl, subεtituted alkynyl or halogen; and R 6 is selected from hydrogen, hydroxy, lower alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl or halogen. 24. A method for modulating insulin-sensitivity and blood glucose levels in a subject, said method compriεing administering to a subject in need of such treatment an amount of a peroxisome proliferator activated receptor-gamma (PPAR-K) antagonist or partial-agonist effective to lower the blood glucose level of said subject. 25. A composition comprising a compound having the structure I and a pharmaceutically acceptable carrier therefor, wherein structure I is as follows: wherein: each of X 1 , X 2 , X 3 , X 4 , X 5 and X 6 is independently selected from carbon, nitrogen, oxygen or sulfur, with the proviso that at least three of the atoms forming the ring are carbon, R 1 is selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, εubstituted alkenylaryl, alkynylaryl, εubstituted alkynylaryl, arylalkyl , εubεtituted arylalkyl , arylalkenyl, substituted arylalkenyl, arylalkynyl, substituted arylalkynyl, poly (alkylene oxide) , substituted poly (alkylene oxide) , poly (alkylene sulfide) , subεtituted poly(alkylene sulfide) , poly(alkylene amine) , substituted poly(alkylene amine) , -OR, -SR, -NR 2 , wherein each R is independently selected from alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, aryl, substituted aryl, alkylaryl, substituted alkylaryl, arylalkyl, substituted arylalkyl, poly(alkylene oxide) , substituted poly (alkylene oxide) , poly(alkylene sulfide) , substituted 40 poly(alkylene sulfide) , poly(alkylene amine) or substituted poly(alkylene amine) ; R 2 is selected from hydrogen, alkyl, subεtituted alkyl, alkenyl, εubstituted alkenyl, alkynyl, substituted alkynyl, aryl, 45 substituted aryl, alkylaryl, substituted alkylaryl, alkenylaryl, substituted alkenylaryl, alkynylaryl, substituted alkynylaryl, arylalkyl, substituted arylalkyl, arylalkenyl, substituted 50 arylalkenyl, arylalkynyl, subεtituted arylalkynyl, oxyalkyl, poly(alkylene oxide) or substituted poly(alkylene oxide); R 3 is selected from hydrogen, hydroxy, halogen, alkoxy, lower alkyl, subεtituted lower 55 alkyl, alkenyl, εubεtituted alkenyl, alkynyl or substituted alkynyl; R 4 is selected from hydrogen, formyl, acyl, lower alkyl or substituted lower alkyl; R 5 iε selected from hydrogen, hydroxy, lower 60 alkoxy, lower alkyl, substituted lower alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl or halogen; and R 6 is selected from hydrogen, hydroxy, lower alkoxy, lower alkyl, substituted lower 65 alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl or halogen.

Inventors: EVANS RONALD M, FORMAN BARRY M, EVANS, RONALD, M., FORMAN, BARRY, M.
Applicants: SALK INST FOR BIOLOGICAL STUDI, THE SALK INSTITUTE FOR BIOLOGICAL STUDIES
Application year: 1996
Main classification: A61K31
IPC codes: A61K31, A61K45, A61P3, C07D257