POTASSIUM ION CHANNEL BLOCKERS

Abstract: The invention provides novel sulphonylurea, sulphonylthiourea and sulphonylguanidine compounds which have the ability to block potassium ion channels regulated by intracellular concentrations of ATP. Methods of synthesis, pharmaceutical compositions and methods for the treatment of conditions such as type II diabetes, cardiac arrhythmias, ischaemic and hypoxic cardiovascular incidents, and cancers are also claimed.

Description: POTASSIUM ION CHANNEL BLOCKERS This invention relates to compounds which have the ability to block potassium ion channels. In particular, the invention relates to sulphonylurea, sulphonylthiourea and sulphonylguanidine compounds which act to block potassium ion channels regulated by intracellular concentrations of adenosine triphosphate (ATP) . The compounds of the invention are therefore useful in the treatment of conditions such as type II diabetes, cardiac arrhythmias, and ischaemic and hypoxic cardiovascular incidents. Background of the Invention Potassium ion channels are responsible for the control and maintenance of many physiological responses, and a wide variety of such channels is known. The many classes and subclasses of potassium ion channels allow for the development of new chemical entities which specifically allow or impede the passage of potassium ions (K + ) through these channels, thus altering the physiological state of the cell. Compounds which have the ability to modulate the passage of K + through a subset of potassium ion channels which is regulated by the concentration of adenosine triphosphate (ATP) are known as K ATP channel blockers or K ATP openers, depending on whether they impede or enhance the passage of K*. Alteration of potassium ion channel flux causes variations in the excitable state of a number of tissues; open potassium channels allow the membrane potential to come closer to the potassium equilibrium potential, so that the membrane potential is more stable and less likely to fire (Cook, 1990) . Potassium ion channels are also associated with the resting potential, and periods of excitability are terminated by these channels. Thus, by modulating the K * ion movement through these channels, the ensuing responses may be of therapeutic benefit. Type II diabetes is an example of a disease that is effectively treated with potassium ion channel blockers of the sulphonylurea type with relative safety. Such drugs include tolbutamide and glyburide. Ideally these drugs display both rapid onset and short duration of action, thus reducing the risk of chronic hyperinsulinaemia, which is a risk factor for atherosclerosis, and of potentially fatal hypoglycaemic incidents. Thus discontinuous exposure appears to be best during long term treatment, because the plasma drug concentration falls below the threshold level during at least part of the night, since desensitisation appears to be associated with the use of high concentrations of and continuous exposure to sulphonylureas (Palmer and Brogden, 1993) . The K xτp blockers alter the regulation of insulin secretion from pancreatic β cells. This effect results from the fact that the K ATP channels which maintain the cell membrane resting potential are generally open in resting conditions, but close due to an increase in intracellular ATP following glucose metabolism. Depolarisation of the β cell membrane occurs upon closure of these channels. This induces the activation of the voltage-dependent calcium channels, promoting an influx of Ca 2+ into the cells, which facilitates the secretion of insulin from β cells (Gopalakrishnan et al, 1993) . These compounds have also been demonstrated to have the ability to alter K + channel flux in a variety of non-pancre tic tissues by blocking ATP dependent channels, and may be beneficial for the treatment of other diseases (Robertson and Steinberg, 1990) . The possibilities for therapeutic effects on the heart are numerous, as during diastole the myocardium exhibits a stable high resting membrane potential which is maintained predominantly by K * channel currents. These currents also regulate the termination of excitation in the myocardium and pacemaker tissue (Cook, 1990) . Thus many conditions requiring both emergency care and post-emergency care have the potential to be ameliorated by agents modulating the relevant K + channel currents associated with the particular problem. Furthermore, under abnormal conditions other parts of the heart, particularly the A-V node and Purkinje fibres, exhibit self excitation, and thus display an intrinsic rhythmic rate of their own which contrasts to the normal rate of contraction. Thus by stabilising the resting membrane potential in these tissues it is possible that these arrhythmias may be controlled. Blockers of the ATP-dependent K + channels may prevent or reduce ischaemia-induced ventricular arrhythmias by preventing loss of K + through these channels, as demonstrated by the effect of glibenclamide (lμM) when tested in rat heart stimulated at 5Hz (Kantor et al, 1987) . Potassium ion channel modulators have demonstrated the ability to induce physiological changes in many tissues. The effects of these modulators in the pancreas and the heart have been outlined above, with specific reference to the K ATP channel blockers. In addition, some K ATP channel openers, such as the non- specific K + opener pinacidil, have been found to have vasodilator activity, and are useful as anti-hypersensitive agents (Cohen, 1986) . Neuronal ATP channels have also demonstrated an association with transmitter release. The neurotransmitter γ-aminobutyric acid (GABA) is released from the substantia nigra, and this release is mediated via K ATP channels. K ATP channel closure induced by sulphonylurea compounds elicits an increase in intracellular Ca 2+ analogous to that described in the pancreas, resulting in the stimulation of GABA secretion (Schmid-Antomarchi et al, 1990) . The potential for the use of ATP channel blockers as anti-cancer agents has recently been highlighted with the finding that in cultured MCF-7 human mammary carcinoma cells, reversible cell-cycle arrest arises from the inhibition of K ATP channels following treatment with K ATP channel blockers such as Glibenclamide. Cells exposed to Glibenclamide exhibited a concentration-dependent inhibition of cell proliferation, with an IC50 value of 50μM. Cell populations exposed to Glibenclamide also exhibited a statistically significant accumulation at the G0/G1 phase in cell cycle distribution. ( oodfork et al, 1995) . We have now found a novel family of sulphonyl compounds which have potassium ion channel blocking activity, and which are useful in the treatment of conditions mediated by potassium ion movement through these channels. Such conditions include, but are not limited to, type II diabetes, cardiac arrhythmias ischaemic and hypoxic cardiovascular incidents, and cancers. Summarv of the Invention According to one aspect of the present invention there is provided a sulphonyl compound of general formulae selected from the group consisting of formulae I, II, III, IV and V: III IV where R represents a substituted or unsubstituted alkylaryl, benzyl or alkyl group; X represents oxygen, sulphur, amine, or guanidine; represents nitrogen or carbon; and Y, Q and Z independently represent hydrogen, halogen, amine, alkyl, hydroxy, alkoxy, sulphonylurea, sulphonylthiourea or sulphonylguandine, where each of Y, Q and Z may be the same or different, with the proviso that in formula I, when X is O, Y and Z are not both hydrogen, and in formula IV, X is not O, Y and Z are not CH 3 , Q is not H, and R is not C 6 H 4 CH 3 . By "alkylaryl" is meant a group having a single 6-membered aromatic ring. The ring moiety of the alkylaryl group or the benzyl group may be substituted with groups such as chlorine, bromine, ethyl, or methoxy. The alkyl moiety of the alkylaryl group may be straight or branched, and it is also preferably hydrophobic; it may be of substantial chain length, for example up to 20 carbons. By "alkyl" is meant a straight or branched, saturated or unsaturated, substituted or unsubstituted alkyl chain, which may again be long, for example up to 20 carbons. Preferably the chain is saturated. Where the chain is substituted, the substituent groups are preferably hydrophobic. A very wide variety of alkyl or alkylaryl substituents, including long chain substituents, has been used in known sulphonylurea, sulphonylthiourea and sulphonylguanidine groups, and similar groups are suitable for use in the present invention. By "alkoxy" is meant a substituted or unsubstituted alkoxy group of 1 to 6 carbon atoms, preferably 1 to 3 carbon atoms. The alkyl moiety may be substituted or unsaturated, and may be straight or branched. Where X is amino or guanidine, or Y, Q or Z is amine, these may optionally be substituted. The halogen is preferably chlorine, bromine or iodine, and more preferably is bromine. In general formulae I to IV, Y, Q or Z as appropriate may be sulphonylurea, and where this is the case, the sulphonylurea group is preferably symmetrical with the sulphonylurea chain which is substituted by the R group. Preferred compounds of the invention are those of the general formulae I to V as defined above wherein R represents an alkylaryl group; X represents oxygen or sulphur; W represents nitrogen or carbon; and Y, Q and Z independently represent hydrogen, halogen, amino, alkyl, hydroxy, sulphonylurea or alkoxy, where each of Y, Q and Z may be the same or different. In a particularly preferred embodiment, the compound of the invention is of general formula I, II or III, and the pyridine ring is substituted by an ethoxy group. Prior art studies on sulphonylurea, sulphonylthiourea and sulphonylguanidine compounds have concentrated on varying the R group. Compounds in which the urea or guanidine chain is linked to a pyridine group have received relatively little attention. R groups of the types used in the prior art are suitable for use in the present invention. Some preliminary evidence suggests that certain metabolites of compounds according to the invention may also have the ability to block potassium ion channels, and it will be clearly understood that metabolites having this biological activity fall within the scope of the invention. Such compounds are referred to herein as "active metabolites" . According to a second aspect of the present invention there is provided a process for the preparation of compounds of the general formulae I, II, III, IV and V as defined above which comprises the step of reacting a compound of the general formulae VI, VII, VIII or IX respectively: wherein W, Y, Z, and Q are as defined above, with a compound of the general formula X: χ=C=N—S—R II o X wherein X and R again are as defined above. The reaction of the heterocyclic amines of the general formulae VI to IX with the isocyanate or isothiocyanate of general formula X may be carried out in any suitable solvent, such as tetrahydrofuran or toluene. The reaction is typically conducted between room temperature and 100°C in the absence or presence of base. Reactants used in the preparation of compounds of the present are largely known in the chemical literature. According to a third aspect, the invention provides a pharmaceutical composition comprising a compound of formula selected from general formulae I, II, III, IV and V as defined above, together with a pharmaceutically- acceptable carrier. Methods and pharmaceutical carriers for preparation of pharmaceutical compositions are well known in the art, as set out in textbooks such as Remington's Pharmaceutical Sciences, 17th Edition, Mack Publishing Company, Easton, Pennsylvania, USA. According to a fourth aspect, the invention provides a method of treatment of a condition mediated by alteration of potassium ion channel flux, comprising the step of administering an effective amount of a compound of formula selected from the group consisting of general formulae I, II, III, IV and V, or an active metabolite thereof, to a mammal in need of such treatment. Preferably the condition mediated by potassium ion channel flux is selected from the group consisting of type II diabetes, cardiac arrhythmias, and ischaemic and hypoxic cardiovascular incidents. Brief Description of the Figures Figure 1 summarises two synthetic schemes used for preparation of compounds of the invention of the type set out in Table 3 below; Figure 2 compares the effect on plasma glucose level of three compounds according to the invention, vehicle alone and Glibenclamide; Figures 3a and 3b show the relationship between dose, weight gain, and area under the curve for two compounds of the invention. The error bars represent standard error of the mean; Figure 4 shows the responses of isolated guinea pig atria to compounds of the invention. The error bars represent standard error of the mean; and Figure 5 shows the effect of a compound of the invention on thymidine uptake by MCF-7 tumour cells. The error bars represent standard error of the mean. Detailed Description of the Invention The invention will now be described with reference to the following non-limiting examples. Representative examples of compounds of the invention are given in Tables 1 to 5 below. The compound of Example 1, 4, 6-dimethyl-2- pyrimidyl-N- [4-methylphenyl) sulphonyl] -N' -urea, is known, and is specifically excluded from the scope of the invention. It is referred to herein for purposes of comparison only. Table 1 Table 2 II Table 3 111 Table 4 IV Table 5 V Example 1 4, 6-dimethyl-2-pyrimidyl-N- [4-methylphenvD sulphonyl] -N' -urea As stated above, this compound is known, and its synthesis is included as a comparative example. 2-Amino-4, 6-dimethylpyrimidine (200mg, 1.6mmol) was stirred in toluene (4ml) under a nitrogen atmosphere. p-Toluenesulphonylisocyanate (0.3ml, 460mg, 2.3mmol) was added and stirring continued for 4 hours at room temperature. The mixture was then filtered and the precipitate washed with toluene and chloroform to give white 4, 6-dimethyl-2-pyrimidyl-N- [4-methylphenyl) - sulphonyl] -N' -urea (500 mg, 96% yield), m.p. 205-207°C, lit. m.p. 221°C dec. v mΛX (KBr disc) 3158w, 1698s, 1605s, 1556m, 1449s, 1350s, 1165s, 1087m, 875w, 814w, 681w, 558s cm "1 . X H n.m.r. spectrum (300 MHz) : δ 2.45, s, 3 x CH 3 ; 6.75, 1H' ; 7.31 - 8.00, AA'BB', 4H (H3, H5 and H2, H6 respectively); 8.83, brs, NH. Mass spectrum: m/z 321 (M+l, 100%), 256 (3), 150 (4), 124 (16) . Example 2 N- I (4-Methylphenyl) sulphonyl] -N' - (6- ethoxypyrid-2-yl)urea (Compound 1-4) 2-Amino-6-ethoxypyridine (1.7g, 12.7mmol) was stirred in toluene (20ml) at room temperature under a nitrogen atmosphere. N- (4-methylphenyl) sulphonylisothio- cyanate (1.7ml, 2.6g, 13.2mmol) was added slowly, and stirring continued for 2 hours. The precipitate was then removed by filtration, dissolved in sodium hydroxide and then acidified with hydrochloric acid until precipitation. Filtration gave N- [ (4-methylphenyl) sulphonyl] -N' - (6- ethoxypyrid-2-yl)urea (3.59g, 85%) as a white powder, m.p. 197-199°C. (Found: C, 54.1; H, 5.1; N, 12.1; O, 19.3; S, 9.2. C 15 H 17 N 3 0 4 S requires C, 53.7; H, 5.1; N, 12.5; O, 19.1; S, 9.6 %) . v mΛX (KBr disc) 3230w, 3083w, 2981m, 1702s, 1580s, 1454m, 1432s, 1352m, 1246m, 1168s, 1093m, 797m, 687m, 539m cm "1 . X H n.m.r. spectrum (300 MHz) : δ 1.45, t, J CH3 , 7Hz, CH 3 ,; 2.43, ε, CH3; 4.33, q, J CH2 ., 7Hz, CH 2 ,; 6.36-7.51, m, 3H' ; 7.31 -7.97, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 336 (M+l, 100%), 244 (1), 201 (100), 212 (3), 198 (25), 172 (33), 139 (89) . Example 3 4, 5-Diethyl-2-pyrimidyl-N- [4-methylphenvD - sulphonyl] -N' -urea dicarboxylate (Compound 4-16) 2-Amino-4, 5-diethylpyrimidine dicarboxylate (400mg, 1.67mmol) was stirred in toluene (8ml) under a nitrogen atmosphere. p-Toluenesulphonylisocyanate (0.3ml, 460mg, 2.3mmol) was added and stirring continued overnight at room temperature. The mixture was then filtered and the precipitate washed with toluene and recrystallised from chloroform to give white 4, 5-diethyl-2-pyrimidyl-N- [4- methylphenyl) sulphonyl] -N' -urea dicarboxylate (630 mg, 86%), m.p. 163-164°C. (Found: C, 49.6; H, 4.6; N, 12.8; 0, 25.8; S, 6.8. C 18 H 20 N 4 0 7 S requires C, 49.5; H, 4.6; N, 12.8; 0, 25.7; S, 7.3%) . v ffiax (KBr disc) 3221m, 3139m, 2291m, 1734s, 1696s, 1586s, 1470s, 1438s, 1358s, 1297s, 1162s, 1087m, 1022m, 891m, 813m, 709m, 696w, 547m cm "1 . 1 H n.m.r. spectrum (300 MHz) : δ 1.36-1.45, m, 2CH 3 ; 2.44, ε, CH 3 ; 4.40, q, CH 2 , J CH2 , 10.7Hz; 4.47, q, CH2, JCH2, 10.7Hz 7.31-8.00, AA'BB', 4H (H3, H5 and H2, H6 respectively); 9.12, ε, H4. Mass spectrum: m/z 436 (M+l, 100%), 240 ( 63 ) , 198 ( 69 ) . Example 4 N- [ (4-Methylphenyl) sulphonyl] -N' - (6- ethoxypyrid-2-yl) thiourea (Compound 1-24) N- (4-methylphenyl) sulphonylisothiocyanate (1.75g, 8.2mmol) was dissolved in toluene (25ml) at room temperature. 2-Amino-6-ethoxypyridine (l.lg, 8.2mmol) was added, and the mixture refluxed overnight. Upon cooling the precipitate was removed by filtration, washed with toluene and dichloromethane, then air dried. Washing with warm methanol gave N- [ (4-methylphenyl) sulphonyl] -N' - (6- ethoxypyrid-2-yl) thiourea (1.12g, 43%), m.p. 196-197°C. (Found: C, 51.6; H, 4.7; N, 12.0. C 15 H 17 N 3 0 3 S 2 requires C, 51.3; H, 4.9; N, 12.0%) . vmax (KBr disc) 3188w, 3026w, 2984w, 1704w, 1632m, 1538s, 1432s, 1352s, 1218s, 1170s, 1090m, 950w, 789m, 655m, 523m cm "1 . X H n.m.r. spectrum (300 MHz) : δ 1.49, t, J CH3 , 7.05Hz, CH 3 ; 2.44, s, CH 3 ; 4.41, q, J CH2 , 7.14Hz, CH 2 ; 6.46-7.59, , 3H' ; 7.31-7.96, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 351 (M+l, 13%), 336 (8), 318 (5), 238 (5), 226 (14), 214 (27), 198 (65), 181 (25), 172 (31), 157 (41), 139 (100), 125 (30), 111 (19) . Example 5 N- T (4-Methylphenyl)sulphonyl] -N' - (4,6- dimethylpyrimid-2-yl) thiourea (Compound 4-36) 2- Amino-4, 6-dimethylpyrimidine (lg, 8.13 mmol) and N- (4-methylphenyl) sulphonylisothiocyanate (2g, 9.39mmol) were refluxed overnight in toluene (30ml) . Upon cooling the precipitate was removed by filtration, then washed with toluene giving N- [ (4-methylphenyl) sulphonyl] - N' - (4,6-dimethylpyrimid-2-yl) thiourea (2.08g, 76.1%) as opaque crystals, m.p. 160-161°C dec. (note a crystal structure change at 131°C) . (Found: C, 50.4; H, 5.0; N, 16.7. C 14 H 16 N 4 0 2 S 2 requires C, 50.0; H, 4.8; N, 16.7 %) . vmax (KBr disc) 3470w, 3308w, 3159w, 3013w, 2867w, 1613m, 1524s, 1486m, 1430m, 1352ε, 1184m, 1164ε, 1088w, 931w, 814w, 654w, 562m cm "1 . 1 H n.m.r. spectrum (300 MHz) : δ NH; 1.63, ε, 1H' ; 2.31, s, CH 3 ; 2.44, ε, 2xCH 3 ,; 6.40, sbr, NH; 6.79, s, H' ; 7.31-8.01, AA'BB', 4H (H3, H5 and H2, H6 respectively); 8.61, sbr, NH. Mass spectrum: m/z 337 (M+l, 68%), 305 (3), 271 (6), 229 (15), 211 (25), 183 (93), 166 (39), 157 (43), 149 (37), 139 (18), 124 (100) . Example 6 N,N' -Pi- [4-methylphenyl) sulphonyl] -N" (4, 5- diethyl-2-pyrimidyl-dicarboxylate)guanidine (Compound 5-39) N- (4-Methylphenyl) sulphonylisothiocyanate (0.93g, 4.4mmol) was dissolved at room temperature in toluene (20ml) . Diethyl-2-amino-4, 5-pyrimidinedicarboxylate (0.93g, 3.9mmol) was then added and the mixture stirred at room temperature for one hour. Diisopropylethylamine (10 drops) was then added and stirring continued overnight. The precipitate was removed by filtration, washed with toluene and recrystallised from ethanol and water to give N,N' -Di- [4-methylphenyl) sulphonyl] -N' ' (4,5-diethyl-2- pyrimidyl-dicarboxylate)guanidine (670 g, 29%), m.p. 205-206°C. (Found: C, 51.2; H, 4.5; N, 11.6. C 25 H 27 N 5 0 8 S 2 requires C, 50.9; H, 4.6; N, 11.9%). vmax (KBr disc) 3160w, 3076w, 2987w, 1748m, 1716m, 1627ε, 1555ε, 1473m, 1361ε, 1302s, 1173m, 1066w, 917w, 813m, 693m, 546m cm "1 . H n.m.r. spectrum (300 MHz): δ 1.37-1.47, m, 2xCH 3 ,; 2.41, s, CH 3 ; 2.44, s, CH 3 ; 4.42, q, CH 2 , J CH2 , 10.7Hz; 4.50, q, CH 2 , J CH2 , 10.7Hz; 7.20-7.77, AA'BB', 4H (H3, H5 and H2, H6 respectively); 7.26-7.90, AA'BB', 4H" (H3" , H5" and H2", H6" respectively); 9.17, ε, H4; 10.79, brε, NH. Masε εpectrum: m/z 590 (M+l, 99%) , 526 (9) , 454 (19), 436 (100), 391 (9), 371 (15), 282 (12), 257 (10), 240 (40), 225 (13), 197 (15), 171 (30), 157 (75), 139 (53), 125 (80) . Example 7 4-Amino-3-pyridyl-N- [4-methylphenyl) - sulphonyl] -N' -urea (Compound 2-14) 3,4-Diaminopyridine (0.5g, 4.7mmol) was stirred at 60°C in toluene (20ml) under a nitrogen atmosphere. p-Toluenesulphonylisocyanate (0.6ml, 0.92g, 4.7mmol) was added and stirring continued for 3 days at 60°C. The mixture was then allowed to cool to room temperature. The mixture was then filtered and the precipitate washed with toluene and then recrystallised from water to give 4-amino-3-pyridyl-N- [4-methylphenyl) sulphonyl] -N' -urea (515mg, 36%), m.p. 150-152 jC. v mβx (KBr disc) 3447m, 3394m, 3343m, 3209s, 3144s, 3009m, 1666s, 1595ε, 1567s, 1422ε, 12538, 1158s, 1057s, 827s, 670s, 534s cm "1 . X H n.m.r. spectrum (300 MHz) : δ 2.36, s, CH 3 ; 7.01, brs, NH 2 ; 6.70-7.72, m, 3H' ; 7.14 -7.73, AA'BB', 4H (H3, H5 and H2, H6 respectively); 7.61, s, NH. Mass spectrum: m/z 304 (M-2, 59%), 281 (10), 256 (10) . 171 (47), 155 (30), 109 (100), 91 (43), 81 (14), 55 (37) . Example 8 N- [ (4-Methylphenyl) sulphonyl] -N' - (6- aminopyrid-2-yl)urea (Compound 1-7) 2, 6-Diaminopyridine (200mg, l.βmmol) and N- (4-methylphenyl) sulphonylisocyanate (0.24ml, 0.37g, 1 equiv) were stirred in toluene (10ml) under a nitrogen atmosphere at approximately 0°C for 3 hours. The white gelatinous mixture was filtered, washed with toluene and chloroform, then allowed to dry in a dessicator. The white solid was dissolved in base, filtered, then reacidified and the precipitate collected. The precipitate was then stirred in hot chloroform, cooled, and filtered. The insoluble solid was then washed with ethylacetate and recrystallised from methanol to give N- [ (4-methylphenyl) - sulphonyl] -N' - (6-aminopyrid-2-yl)urea as fine opaque needles (410mg, 73%), m.p. 155-157iC. (Found: C, 48.5; H, 5.2; N, 17.6. C 13 H 14 N 4 .0 3 S. H 2 0 requires C, 48.1; H, 5.0; N, 17.3 %) . v max (KBr disc) 3553w, 3412w, 3271m, 3157m, 2914w, 1657s, 1598s, 1550m, 1449w, 1391m, 1342m, 1245s, 1138s, 1089m, 1054m, 888m, 779w, 541m cm "1 . X H n.m.r. spectrum (300 MHz) : δ 2.41, s, CH 3 ; 3.07, brε, NH 2 ; 7.41-7.46, m, 3H' ; 7.23 -7.89, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 307 (M+l, 63%) , 198 (82), 172 (100), 155 (27). 136 (32), 110 (71). Example 9 N- [ (4-Methylphenyl) sulphonyl] -N' - (6- bromopyrid-2-yl)urea (Compound 1-1) 2-Amino-6-bromopyridine (l.Og, 5.3mmol) was stirred in toluene (20ml) at room temperature under a nitrogen atmosphere. N- (4-methylphenyl) sulphonylisothio¬ cyanate (0.75ml, 1.15g, 5.8mmol) was added slowly, and stirring continued for 2 hours. The precipitate was then removed by filtration, dissolved in sodium hydroxide and then acidified with hydrochloric acid until precipitation which was collected by filtration. Recrystallisation from ethanol gave N- [ (4-methylphenyl) sulphonyl] -N' - (6- bromopyrid-2-yl)urea (1.77g, 83%) aε a white powder, m.p. > 310°C. (Found: C, 42.3; H, 3.3; N, 11.2. C 13 H 12 N 3 0 3 SBr requires C, 42.2; H, 3.3; N, 11.4 %) . v max (KBr disc) 3436m, 3404m, 1630s, 1561s, 1509ε, 1385s, 1271s, 1223s, 1137s, 1066s, 869m, 789m, 665m, 554s cm "1 . X H n.m.r. spectrum (300 MHz): δ 2.43, s, CH 3 ; 2.58, sbr, 2 x NH; 7.09-7.47, m, 3H' , 4H' and 5H' ; m, 3H; 7.32-7.96, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Masε spectrum: m/z 371 (M+l, 1%), 218 (18), 201 (100), 199 (99), 173 (97), 137 (10) , 121 (53) . Example 10 2-Pyrimidyl-N- [4-methylphenyl) sulphonyl] - N' -urea-4.5-dicarboxylic acid (Compound 4-15) 4, 5-Diethyl-2-pyrimidyl-N- [4-methylphenyl) - sulphonyl] -N' -urea dicarboxylate (200mg, 0.46mmol) was dissolved in sodium hydroxide (lM,15ml) and heated with stirring for 1 hour. The solution was allowed to cool, then acidified with hydrochloric acid (6M) , the precipitate collected by filtration and washed with water then chloroform. Drying gave white 2-pyrimidyl-N- [4- methylphenyl) εulphonyl] -N' -urea-4, 5-dicarboxylic acid (112mg, 65%), m.p. 194-196jC. (Found: C, 40.4; H, 3.6; N, 13.1. C 14 H 12 N 4 0 7 S.2H 2 0 requires C, 40.4; H, 3.4; N, 13.5 %) . v max (KBr disc) 3410brm, 3130brm, 1726s, 1687s, 1583s, 1470s, 1344s, 1235m, 1164s, 1086m, 961w, 818m, 710m, 546m cm "1 . X H n.m.r. spectrum (300 MHz) : δ 2.44, s, CH 3 ; 3.40, sbr, NH; 7.31-7.99, AA'BB', 4H (H3, H5 and H2, H6 respectively); 9.13, s, H6'; 10.37, brs, NH; 12.02, brs, NH. Mass spectrum: 212 (4), 198 (5), 189 (9), 183 (2), 172 (100), 155 (8), 135 (7) . Example 11 N- [ (4-Methylphenyl) εulphonyl) 1 -N' - (3- pyridyl) thiourea (Compound 2-41) N- (4-Methylphenyl) sulphonylisothiocyanate (200mg, 0.94mmol) and 4-aminopyridine (90mg, l.Ommol) was stirred in toluene (10ml) for 30 minutes, potassium hydroxide was added and stirring continued for a further hour. The mixture was then refluxed at 120°C for 5 hours then allowed to cool. The precipitate was removed by filtration, dissolved in water and acidified with dilute hydrochloric acid. N- [ (4-Methylphenyl) sulphonyl) ] -N' - (3-pyridyl) thio¬ urea (221mg, 72%) was then air dried (m.p.129-132°C) . " max < KBr disc) 3357m, 3259m, 3051w, 2633w, 1597w, 1558w, 1495w, 1389w, 1302m, 1155s, 1096w, 904w, 818m, 704w, 534m cm "1 . X H n.m.r. spectrum (300 MHz): δ 2.41, s, CH 3 ; 6.93, sbr, NH; 7.26-7.79, m, 3H' ; 7.27-7.79, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 308 (M+l, 75%), 292 (27), 214 (97), 200 (10), 172 (100), 155 (91), 135 (7) , 123 (24) . Example 12 N- f (4-Methylphenyl) sulphonyl] -N' - (2- pyridyl) thiourea (Compound 1-42) 2-Aminopyridine (0.9g, 9.6mmol) and N-(4- methylphenyl) sulphonylisothiocyanate (2g, 9.4mmol) were refluxed overnight in toluene (35ml) . Upon cooling the precipitate was removed by filtration, then washed with toluene giving N- [ (4-methylphenyl) sulphonyl] -N' - (2- pyridyl) thiourea (1.4g, 49.3%) as a white powder, m.p. 154-155°C dec. (Found: C, 49.5; H, 4.5; N, 13.3. C 13 H 13 N 3 0 2 S 2 .1/2H 2 0 requires C, 49.4; H, 4.5; N, 13.3 %) . v aax (KBr disc) 3482w, 3268w, 3029brw, 1640m, 1606s, 1559m, 1474m, 1388m, 1340m, 1149s, 1087m, 959w, 727m, 656m, 523m cm "1 . X H n.m.r. spectrum (300 MHz) : δ 2.43, s, CH 3 ; 7.04-8.29, m, 4H' ; 7.30-7.98, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 308 (M+l, 66%) , 274 (5), 242 (7), 214 (100), 172 (10), 165 (13), 155 (65), 135 (37) , 123 (96) . Example 13 N- [ (4-Methylphenyl) sulphonyl] -N' - (4- pyridyl) thiourea (Compound 3-23) 4-Aminopyridine (250 mg, 266mmol) and N- (4- methylphenyl) sulphonylisothiocyanate (500 mg, 235mmol) were stirred in tetrahydrofuran (20ml) for 2 hours at room temperature. The yellow precipitate was then removed by filtration and washed with tetrahydrofuran leaving small opaque plates of N- [ (4-methylphenyl) sulphonyl] -N' - (4- pyridyl) thiourea (390mg, 54%) m.p. 124-125°C dec. (Found: C, 50.5; H, 4.5; N, 13.9. C 13 H 13 N 3 0 2 S 2 requires C, 50.8; H, 4.3; N, 13.7%) . v max (KBr disc) 3304m, 3203m, 2055m, 1644s, 1599m, 1530s, 1445s, 1326m, 1278s, 1145s, 1077s, 979s, 812s, 540s cm "1 . 1 H n.m.r. spectrum (300 MHz) : δ 2.39, s, CH 3 ; 6.71-9.33, m, 4H' ; 7.24-7.94, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 306 (M-l, 2%), 260 (9), 246 (36), 214 (73), 198 (11), 172 (29), 155 (18), 135 (53), 123 (100) . Example 14 N- [ (4-Methylphenyl) sulphonyl] -N' - (6- bromopyrid-2-yl) thiourea (Compound 1-21) 2-Amino-6-bromopyridine (0.9g, 5.2mmol) and N- (4-methylphenyl) sulphonylisothiocyanate (l.lg, 5.16mmol) were refluxed overnight in toluene (35ml) . Upon cooling the precipitate was removed by filtration, then washed with toluene and dichloromethane giving N- [ (4-methylphenyl) - sulphonyl] -N' - (6-bromopyrid-2-yl) thiourea (l.lg, 55.2%) as opaque crystals, m.p. 175-176°C dec. (Found: C, 40.5; H, 3.1; N, 10.6. C 13 H 12 N 3 0 2 S 2 Br requires C, 40.4; H, 3.1; N, 10.9 %) . v max (KBr disc) 3270w, 3076w, 3000w, 2860w, 1592m, 1517m, 1439m, 1347m, 1196m, 1159s, 1090w, 952w, 793w, 658w, 544m cm "1 . X H n.m.r. spectrum (300 MHz): δ 2.44, s, CH 3 ; 7.20-7.57, m, 3H' ; 7.32-7.99, AA'BB', 4H (H3, H5 and H2, H6 reεpectively) . Mass spectrum: m/z 388 (M+2, 13%), 386 (M, 13%), 232 (6), 214 (27), 201 (11), 175 (95), 173 (100), 155 (18), 135 (9), 125 (15) . Example 15 N- [ (4-Methylphenyl) sulphonyl] -N' - (4- aminopyrid-3-yl) thiourea (Compound 2-34) 3,4-Diaminopyridine (0.19g, 1.74 mmol) was refluxed with N- (4-methylphenyl) sulphonylisothiocyanate (0.3g, l.δmmol) in toluene (10ml) overnight. The precipitate was then removed by filtration and washed thoroughly with cold dichloromethane, to give N-[(4- methylphenyl) sulphonyl] -N' - (4-aminopyrid-3-yl) thiourea (200mg, 37.6%), m.p. 185-187°C. v max (KBr disc) 3418w, 3291m, 3152s, 3030m, 2947m, 1654s, 1565m, 1488s, 1337ε, 1241w, 1155s, 1090m, 971w, 816w, 747w, 660w, 545w cm "1 . n.m.r. spectrum (300 MHz) : δ 2.45, s, CH 3 ; 7.00-7.81, m, 3H' ; 7.33-7.94, AA'BB', 4H (H3, H5 and H2, H6 respectively); 8.34, sbr, NH2. Mass spectrum: m/z 323 (M+l, 5%), 289 (13), 231 (5), 214 (55), 200 (17), 189 (23), 172 (79), 152 (54), 138 (43), 125 (14), 111 (100) . Example 16 N- [ (4-Methylphenyl) sulphonyl] -N' - (6- aminopyrid-2-yl) thiourea (Compound 2-27) 2, 6-Diaminopyridine (0.5g, 4.58mmol) and N- (4-methylphenyl) sulphonylisothiocyanate (lg, 469mmol) were stirred in tetrahydrofuran (20ml) for 5 days at room temperature. The precipitate was then removed by filtration, and dissolved in dilute sodium hydroxide (3M) . Upon acidification with dilute hydrochloric acid (3M) the mixture was allowed to stand at 5°C overnight. The precipitate was then collected by filtration giving fine pale yellow needles of N- [(4-methylphenyl) sulphonyl] -N' - (6- aminopyrid-2-yl) thiourea (1.45g, 89.5%), m.p. 136-138°C dec. (Found: C, 44.5; H, 4.5; N, 15.9. C 13 H 14 N 4 0 2 S 2 .1.5 H 2 0 requires C, 44.7; H, 4.9; N, 16.0 %) . v max (KBr disc) 3412m, 3150m, 2993w, 1659m, 1604s, 1491w, 1407m, 1273m, 1152s, 1088m, 996w, 729m, 604w, 532w cm "1 . X H n.m.r. spectrum (300 MHz): δ 2.42, s, CH 3 ; 5.15, sbr, NH; 6.22-7.42, m, 3H' ; 7.29-7.98, AA'BB', 4H (H3, H5 and H2, H6 respectively); 10.33, sbr, NH. Mass spectrum: m/z 323 (M+l, 13%), 214 (69), 200 (17), 172 (3), 155 (25), 138 (16) , 125 (3) , 110 (100) . Example 17 N.N' -Pi- [ (4-methylphenyl) sulphonyl] -N' ' - (2.6-pyridyl) thiourea (Compound 1-39) N- (4-Methylphenyl) sulphonylisocyanate (2.0g, 9.4mmol) was dissolved in toluene (35ml) at room temperature. To the stirring mixture was added 2, 6-Piaminopyridine (lg, β.lmmol) and the solution refluxed overnight. Upon cooling the precipitate was removed by filtration, washed with toluene and recrystallised from ethanol and water to give N,N' -di- [ (4-methylphenyl) - sulphonyl] -N' '- (2, 6-pyridyl) thiourea (887mg, 21%), m.p. 186-188°C. v max (KBr disc) 3346m, 3217w, 3100w, 2995w, 1657s, 1630s, 1526m, 1464s, 1401s, 1307m, 1275s, 1141s, 1078s, 840m, 765m, 673m, 551m cm "1 . X H n.m.r. spectrum (300 MHz) : δ 2.39, s, CH 3 ; 2.41, ε, CH 3 ; 5.82, 8, NH; 5.85, ε, NH; 6.38-7.31, m, 3H' ; 7.22 -7.88,2 x AA'BB', 2 x 4H (H3, H5 and H2, H6 respectively) 8.19, sbr, NH; 8.22, sbr, NH. Mass spectrum: m/z 534 (M-l, <0.1%), 411 (7), 371 (12), 257 (11), 229 (6), 172 (3), 153 (6), 135 (15), 110 (100) . Example 18 N,N' -Pi- [ (4-methylphenyl) sulphonyl] -N' ' - (4, 6-dimethylpyrimid-2-yl)cruanidine (Compound 5-40) N- [ (4-Methylphenyl) sulphonyl] -N' -(4,6- dimethylpyrimid-2-yl) thiourea (l.lg, 3.4mmol) and 1, 3-dicyclohexylcarbodiimide (1.2g, 4.6mmol) were stirred at room temperature in acetonitrile (10ml) . To this stirring mixture was added cyanamide (0.3g, 7mmol) and a catalytic amount of diisopropylethylamine (5 dropε) , and the mixture allowed to stir at room temperature for 3 days. The solvent was then removed under vacuum, sodium hydroxide (1M, 10ml) added with stirring and any solid removed by filtration. The aqueous phase was then acidified with hydrochloric acid (1M) , and the precipitate collected by filtration. Recrystallisation from methanol gave N,N'-di- [ (4-methylphenyl) sulphonyl] -N' ' - (4, 6-dimethylpyrimid-2- yDguanidine (157mg, 10%), m.p. 171-172°C dec. v max (KBr disc) 3267w, 3232w, 3065w, 2925w, 1634s, 1618s, 1610s, 1541s, 1492m, 1438m, 1346m, 1164m, 1075w, 896w, 815w, 749w, 674w, 547 cm "1 . X H n.m.r. spectrum (300 MHz): δ 2.41, s, 2 x CH3'; 2.48, s, CH 3 ; 2.50, s, CH 3 ; 2.59, εbr, NH; 6.88-8.07, m, 9H; 10.21, sbr, NH. Mass spectrum: m/z 474 (M+l, 91%), 426 (100), 363 (16), 320 (100), 272 (57), 258 (10), 234 (7), 209 (29), 189 (71), 166 (15), 149 (9), 124 (73) , 108 (11) . Example 19 Further compounds according to Table 3 Synthesis of Compound 3.23 is described in Example 13. Two general reaction schemes were utilised for preparation of several additional compounds according to Table 3 above. These synthetic schemes are summarised in Figure 1. Some of the materials used are not readily commercially available, and were prepared according to methods described in the literature, and briefly summarised in Paragraphs a) to f) and i) to 1) . These starting materials were then used to prepare compounds of the invention, as described in Paragraphs g) , h) and m) to o) . a) Pyridine-N-oxide (Den Hertog and Combe, 1951) Pyridine (70ml) was mixed with hydrogen peroxide (180ml) and acetic acid (180ml) . The mixture was stirred at 90°C overnight, then allowed to cool. The volume was reduced under vacuum, and the solution basified and then extracted with chloroform. The chloroform was then removed under reduced pressure, giving white crystals of pyridine- N-oxide (68g, 82%) . b) Mercury salt (Van Ammers and Pen Hertog, 1958) Pyridine-N-oxide (30g) was disεolved in acetic acid (250ml) . With stirring, Mercury II acetate was added and the mixture heated to 130°C for 5 hrs, then allowed to cool. The mixture was then poured into a 10% acetic acid/water solution, and saturated sodium chloride added. The precipitate was filtered, washed, with water and air dried, giving white 2, 6-dimercury pyridine-N-oxide. c) 2, 6-dibromopyridine-N-oxide (Van Ammers and Pen Hertog, 1958) The crude mercury εalt (40g) was stirred in water (150ml) at 50°C. A solution of bromine (10ml) in saturated sodium bromide (50ml) waε added dropwise and the solution εtirred at 50°C for a further 1.5 hrs. After cooling the solution was basified with sodium hydrogen carbonate and extracted with chloroform. Removal of the chloroform under reduced pressure left a yellow oil of 2, 6-dibromopyridine- N-oxide. d) 4-nitro-2.6-dibromopridine-N-oxide (Van Ammers and Pen Hertog, 1958) 2, 6-dibromopyridine-N-oxide (6.28g) was dissolved in concentrated sulphuric acid (20ml) with stirring. A mixture of concentrated sulphuric acid (20ml) and concentrated nitric acid (10ml) was then continuously added dropwise. The solution was then heated at 90°C for 3 hours with stirring, poured onto ice and then basified with sodium hydroxide. The solution was then continuously extracted with ether, and recrystallised from ethanol to give pale yellow leaflets of 4-nitro-2, 6-dibromopyridine-N- oxide (1.2g, 16%), m.p. 222°C-224°C. e) 4-amino-2.6-dibromopyridine (Van Ammers and Pen Hertog, 1958) A mixture of 4-nitro-2, 6-dibromopyridine-N-oxide (1.2g), iron powder (1.2g) and acetic acid (15ml) were stirred and heated to 100°C for 1 hour. Upon cooling the mixture was basified with 3 molar sodium hydroxide and continuously extracted with ether. Removal of the ether under reduced pressure left a white crystalline product of 4-amino-2, 6-dibromopyridine (l.Og, 99%), m.p. 212-214°C. f) 4-amino-2.6-diethoxypyridine 4-amino-2, 6-dibromopyridine (500mg) was mixed with εolid εodium hydroxide (508mg) and ethanol (15ml) in a bomb. The bomb waε then heated at 160C for 6 hrε, allowed to cool, water added and solvent reduced under vacuum. The mixture was then extracted with water and ether. Removal of the ether left an oil of 4-amino-2, 6- diethoxypyridine (220mg, 61%) . g) N- r (4-Methylphenyl) εulphonyl] -N' - (2.6- diethoxypyrid-4-yl)urea (Compound 3-8) 4-amino-2, 6-diethoxypyridine (750mg) waε εtirred in toluene (15ml) at 50°C, p-tolueneisocyanate was added dropwise and the mixture stirred for a further 2 hours at 50°C under nitrogen. The mixture was then stirred for 2 days at room temperature. The precipitate waε removed by filtration, and washed with toluene and hot chloroform giving a white crystalline solid of N- [ (4-Methylphenyl) - εulphonyl] -N' - (2, 6-diethoxypyrid-4-yl)urea (838mg) in quantitative yield, m.p. >270°C. λ H n.m.r. spectrum (300 MHz): 6 1.24, t, J^, 7.90Hz, 2xCH 3 ; 2.30, s, CH 3 ; 4.08, q, J^, 7.83Hz, 2xCH 2 ; 6.46- 7.59, m, 3H' ; 7.31-7.96, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Masε spectrum: m/z 351 172 (100), 155 (12), 118 (12). h) N- [ (4-Methylphenyl)sulphonyll -N' - (2.6- dibromopyrid-4-yl) thiourea (Compound 3-24) 4-amino-2,6-dibromopyridine was stirred in toluene (15ml) at 70°C, p-tolueneisothiocyanate was added dropwise and the mixture stirred for a further 8 hours at 70°C under nitrogen. The mixture was then stirred overnight at room temperature. The precipitate was removed by filtration, washed with toluene and recryεtalliεed from water giving a white cryεtalline solid of N- [(4- Methylphenyl) sulphonyl] -N' - (2, 6-dibromopyrid-4-yl)urea (224mg) m.p. 124-125°C. 1 H n.m.r. spectrum (300 MHz) : δ 2.44, s, CH 3 ; 7.26, ε, 2H' ; 7.34-7.83, AA'BB', 4H (H3, H5 and H2, H6 respectively) . Mass spectrum: m/z 465 (M, <1%) , 200 (7), 172 (100), 155 (9). i) 2-Bromopyridine-N-oxide (Pen Hertog, Kolder and Combe, 1951) 2-Bromopyridine (12g) , hydrogen peroxide (30%, 90ml) and acetic acid (90ml) were heated at 55-60C for 8 days with stirring. The solvent was then reduced under vacuum, water added, and the process repeated 3 or 4 times, to remove all solvent. A greeniεh oil/solid of 2- Bromopyridine-N-oxide (2.2g,20%) was obtained. j) 2-Bromo-4-nitro-pyridine-N-oxide (Pen Hertog, Kolder and Combe, 1951) 2-Bromopyridine-N-oxide (28.36g) was carefully dissolved in concentrated sulphuric acid (43ml) with stirring. A mixture of concentrated sulphuric acid (65ml) and concentrated nitric acid (43ml) was added dropwise, and the mixture refluxed for 3 hours and allowed to cool to room temperature. The mixture was then poured on to ice and the precipitate removed by filtration , washed with water and air dried leaving a fine yellow powder of 2-Bromo-4-nitro-pyridine-N-oxide (14.08g, 63%), m.p. 145- 147°C. k) 2-Bromo-4-aminopyridine (Pen Hertog, Kolder and Combe, 1951) 2-Bromo-4-nitro-pyridine-N-oxide (4g) was stirred in acetic acid (65ml) at room temperature and iron powder (4g) added. The mixture was then heated to 100°C for 1 hour, allowed to cool to room temperature and diluted with water. The mixture was then basified with sodium hydroxide (3M) and continuously extracted with ether, giving a white crystalline solid of 2-Bromo-4-aminopyridine (3.1g) in quantitative yield, m.p. 97-99°C. 1) 2-Ethoxy-4-aminopyridine (Pen Hertog, Kolder and Combe, 1951) 2-Bromo-4-aminopyridine (4g) . solid sodium hydroxide (4g) and ethanol (15ml) were heated in a bomb at 160°C for 6 hours and allowed to cool. Water was then added and the solvent partially removed under reduced pressure. The mixture was then extracted with ether and the solvent removed, leaving a clear oil of 2-Ethoxy-4- aminopyridine (1.64g, 76%) which slowly crystallised into colourlesε needleε, m.p. 88-89°C. m) N- [ (4-Methylphenyl)sulphonyl] -N' - (2-ethoχypyrid- 4-yl)urea (Compound 3-4) 4-amino-2-ethoxypyridine (518mg) was stirred in toluene (15ml) at 50°C, p-tolueneisocyanate was added dropwise and the mixture stirred for a further 2 hours at 50°C under nitrogen. The mixture was then stirred for 2 days at room temperature. The precipitate was removed by filtration, and washed with toluene and hot chloroform giving a white crystalline solid of N- [ (4-Methylphenyl) - sulphonyl] -N' - (2-ethoxypyrid-4-yl)urea (1.2g, 93%), m.p. 146-148°C. H n.m.r. spectrum (300 MHz): 6 1.30, t, J CT:) , 7.77Hz, CH 3 ; 2.45, s, CH j ; 4.29, q, J^, 7.87Hz, CH 2 ; 6.95-8.19 m, 3H' ; 7.44-7.98 AA'BB', 4H (H3, H5 and H2, H6 reεpectively) . Mass spectrum: m/z 335 (M+l, 29%), 198 (100), 172 (23), 155 (29) , 139 (88) , 111 (19) . n) N- [ (4-Methylphenyl) sulphonyl] -N' - (2-ethoxypyrid- 4-yl) thiourea (Compound 3-15) 4-amino-2-ethoxypyridine (550mg) was εtirred in toluene (15ml) at 70°C, p-tolueneisothiocyanate was added dropwise and the mixture stirred for a further 8 hours at 70°C under nitrogen. The mixture was then stirred overnight at room temperature. The precipitate was removed by filtration, and washed with toluene and hot chloroform giving a white crystalline solid of N- [ (4-Methylphenyl) - εulphonyl] -N' - (2-ethoxypyrid-4-yl) thiourea (1.4g, 98%), m.p. 152-153°C. (Found: C,51.3; H, 4.9; N, 11.7. C 15 H 17 N 3 0 3 S 2 requireε C, 51.3; H, 4.9; N, 12.0 %) . H n.m.r. εpectrum (300 MHz): δ 1.19, t, J CH ,, 7.67Hz, CH 3 ; 2.27, ε, CH 3 ; 3.96, q, J CT3 , 7.47Hz, CH 2 ; 6.24-7.69, m, 3H' ; 7.23-7.62, AA'BB', 4H (H3, H5 and H2, H6 reεpectively) . Mass spectrum: m/z 351 (M+l, 90%), 214 (80), 181 (9), 172 (16), 155 (26), 139 (100), 111 (20) . o) N- [ (4-Methylphenyl) sulphonyl] -N' - (2-bromopyrid- 4-yl)urea (Compound 3-1) 4-amino-2-bromopyridine (200mg) was stirred in toluene (15ml) at 50°C, p-tolueneisocyanate was added dropwise and the mixture stirred for a further 2 hours at 50°C under nitrogen. The mixture was then stirred for 2 days at room temperature. The precipitate was removed by filtration, washed with toluene and hot chloroform giving a white crystalline solid of N- [ (4-Methylphenyl) sulphonyl] - N' - (2-bromopyrid-4-yl)urea (140mg, 36%), m.p. 144-146°C. (Found: C, 38.6; H, 3.3; N, 10.1; S, 8.0. C 13 H 12 N 3 0 3 SBr.2H 2 0 requireε C, 38.4; H, 3.9; N, 10.3; S, 7.9 %) . X H n.m.r. spectrum (300 MHz): δ CH 3 ; 2.28, s, CH 3 ; 7.16-7.90, m, 3H' ; 7.24-7.66, AA'BB', 4H (H3, H5 and H2, H6 reεpectively) . Maεε spectrum: m/z 370 (M, 14%), 198 (100), 173 (94), 155 (34), 135 (16), 109 (10) . Example 20 Hypoglycaemic Activity of Compounds of the Invention The biological activity of representative compounds of the invention was evaluated by means of several different tests. The activity of compounds of this invention as hypoglycaemic agents was measured and compared with the activity of the known compound of Example 1, using methods described in the literature. Thus normal, fed male Sprague Pawley rats weighing between 275 and 375g were studied for changeB in blood sugar levels and alterations to mean arterial blood pressure following administration of representative compounds of the invention suspended in a 5% solution of gum acacia by oral gavage at a dose of 1.6 mg/kg. The concentration of each compound was 4mg in 10ml, so that each rat received 1.1-1.5 ml to obtain the desired dose on a body weight basis. The blood sugar levels were recorded for each rat immediately prior to adminiεtration, and at 1, 2, and 3 hr following administration of the test compounds. Blood glucose levels were determined on blood samples taken from the tail, and analysed on an Ames blood glucometer, model 5626, and Ames, 2627 blood glucose strips (number 2) . The results are recorded in Table 6, and show that the compounds of the invention posseεs substantial hypoglycaemic activity, at least comparable to that of compound of Example 1. These results also show that the onset and the duration of the hypoglycaemic activity are both affected by the specific nature of the heterospecific substitution. It is apparent that generally the heterocyclic sulphonylthioureas have greater activity than the sulphonylureas. Without wishing to be bound by any proposed mechanism for the observed effect, we believe that this may be associated with differences in lipid permeability. Table 6 Example 21 Effect of Compounds of the Invention on Glucose Tolerance and Weight Gain Representative compounds were evaluated for their effect on glucose tolerance following oral administration at doseε ranging from 0.005mg/kg to 100 mg/kg. Normal male Sprague-Pawley rats were dosed with a test compound daily for five days, and were fasted overnight prior to dayε 1 and 5. Control animalε were given ether vehicle only, or Glibenclamide (glyburide; N- [4- (β- ) (2-methoxy-5- chlorobenzamido) ethyl)benzoεulphonyl) -N' -cyclohexylurea] ; 2mg/kg/day) . Blood was collected on day zero and on days one and five at 0, 0.5, 1, 1.5 and 2 hours after drug administration. Blood glucose concentrationε were then measured in duplicate using standard glucose oxidase analysis. The samples were assayed using a Hitachi U-200 Spectrophotometer at a wavelength of 505nm. The weight gain of each rat was also measured over the five day period, and all resultε were compared to both the negative control group (doεing with vehicle only) and the poεitive control group (dosing with Glibenclamide) . It was found that at higher doses of drug, including Glibenclamide (the positive control) , the rats decreased their observed weight gain over a five day period when compared to rats dosed with vehicle only (negative control) . Doses of drugs administered were minimised so as to reduce differences in weight gain whilεt retaining hypoglycaemic activity. The results are summarised in Figures 3a and 3b, in which the εolid line represents weight gain and the dashed line repreεentε glucoεe tolerance. Each point represents the effect of each dose on the rats glucoεe tolerance or ability to gain weight, expressed as a percentage of the control (normal) response (where control=100% response) . The bars represent standard error. ie. percentage = weight gain/weight gain (control) x 100 and percentages AUC/AUC (control) x 100 Example 22 Cardiac Effects of Compounds of the Invention Preliminary investigations were performed on isolated atrial preparations, and the results obtained using representative compounds of the invention compared to those obtained using the known compound of Example 1 and using Glibenclamide. English short-haired guinea pigs weighing between 200 and 300g were killed by cervical dislocation. The whole heart was quickly removed by dissection and immersed in a Krebs-Henεeleit solution, which had been aerated with carbogen (95% oxygen and 5% carbon dioxide) . The atria were then separated by dissection in Krebs-Henseleit solution, whilst being aerated with carbogen. The atria were then immersed in a 10ml organ bath containing Krebε-Henεeleit solution, maintained at 37°C for the duration of the experiment by the circulation of water through the water jackets of organ baths by a temperature controlled water pump (Braun Thermomix, Germany) , and aerated with carbogen. The tip of the rounded atrium was pierced by the hook of the muscle holder, while the more pointed tip of the other atrium was tied by a thread connecting the free beating atria to a Grass force - displacement isometric transducer (FT03) . Changes in tenεion were measured by isometric force transducers and were recorded on a four channel Grass 7P Polygraph (Grass Instruments, Quincy, Masεachuεettε, USA) which waε calibrated daily such that the atria functioned with a resting tenεion of lg. The rate of beating of the atria waε measured by the tachograph. The activity of each test compound was asεessed and compared to that of glibenclamide. A constant εingle dose of 0.8 ml of a 1 x 10" 4 M solution of each of the test compounds and of glibenclamide or of compound of Example 1 was examined such that the bath concentration was 8 x 10" £ M. Maximum responses were recorded and used in analysis. The results are presented in Figure 4, and demonstrate that most of the test compounds display a comparable or superior response to those obtained with glibenclamide but in most caseε have greater inotropic activity. Compoundε 1-24 and 3-23 εhowed particularly marked inotropic activity. Example 23 Kinetic/Metabolite Studies Plasma samples were collected in order to test the correlation of plasma drug (or metabolite) concentration with hypoglycaemic activity, and to determine whether compounds of the invention were converted in vivo into active metabolites. The plasma levels of test compounds or metabolites also give an indication of rate of metabolism and kinetics of drug action. Blood samples collected from rats treated as above and collected at the same intervals were analysed using an ICI LC1150 HPLC system and a LC1210 UV detector at a wavelength of 227nm. The HPLC system used a Merck LiChroCART 125-4 LiChrosphere 100 RP-18 HPLC and LiChroεphere 100 RP-18 guard columns. The mobile phaεe consisted of 70% acetonitrile and a 30% aqueous εodium phosphate buffer at pH 7.45 and was prepared using standard HPLC grade reagents and distilled water. To improve the resolution of peaks and separation of the compounds a gradient method waε established where changes in the concentration of the required buffers and solvents, and the time at which these changes occur is outlined in Table 7. The volumes are expresεed as percentages of the total flow rate, with the flow rate remaining constant at 1.2ml/hr. Table 7 The data were acquired using the WinChrom Pata Management system and were calibrated uεing stock solutions of the test compounds and Glibenclamide aε an internal standard dissolved in 100% methanol. Plasma (250μL) collected from the rats were extracted into methanol (700μL) and 50μL of Glibenclamide (25/xg/ml) . The mixture was then agitated with a vortex mixer for 15 sec and centrifuged for 10 min. The clear supernatant was then carefully aεpirated for HPLC analysis. The results are outlined in Table 8 and show retention times (minutes) for the compounds and their metabolites as analysed by HPLC after oral administration in Sprague-Pawley rats. The urea compound analysed, Compound 1-4 retained its general structure, and no metabolites were observed in the 4 hour period in which sampleε were collected. The thiourea Compoundε 1-24 and 3-15 however, demonεtrated very different metaboliεm and kinetics, with both the test compounds analysed being metabolised within the first 2 hours after administration resulting in the presence of numerous metabolites in the plaεma. Glibenclamide iε εhown for compariεon only, none of the metabolites observed with the teεt compounds correspond to metabolites of Glibenclamide. Table 8 Retention Times (min) of Compounds of the Invention and their Metabolites Example 24 Effect of Compounds of the Invention on Cell Proliferation The ability of a representative compound of the invention to inhibit proliferation of a tumour cell line was examined. MCF-7 human mammary carcinoma cellε (Woodfork et al, 1995) were grown in 25cm 2 Corning flaεkε in a 5% C0 2 water jacket incubator. The medium uεed for the culture waε RPMI 1640 (ICN) and was supplemented with 10% foetal Bovine serum (Cytosystems) . Cells and medium were examined daily to check for growth and infection. The medium was changed every εecond day. When cellε reached 70-80% confluence, the cells were trypsinized with 0.05% trypsin (1:250, "PIFCO") and 0.02% EPTA. The flasks were firstly washed with a phosphate buffer to remove residual RPMI 1640 medium, then covered with 2mL of the trypsin solution and left in the incubator for 3-5 minutes. The cells were then transferred to a conical tube containing 2mL of medium and 10% foetal bovine serum. The cells were centrifuged at 1000 rp for 3 minutes and the εupernatant removed. 5ml of the medium was then added to the pellet of cellε and reεuεpended with a 2 ml pipette. Approximately lOμL of medium containing the cells waε placed in a Neubauer haemocytometer, and the cellε were counted in the 4 x 1 mm 2 grid per chamber. Cellε were then diluted to contain 60 x 10 4 cells to be added to "Corning" 24 well plates (16mm diameter) . Wellε reεpectively containing the control group and lOOμM compound 1-24 were established, and 3 wells/group/day were used to perform the cell growth study. Each well contained 500μL of medium or of compound 1-24. Plates were incubated for 24 hours before counting. The medium was aspirated with different pipette tips for the different study groups. lOOμL of 0.02% EDTA εolution waε added to each well and incubated for 5 minuteε. 200μL of trypεin-EPTA waε then added, and the cells incubated for a further 5 minutes, aspirated, and placed in 0.5mL Eppendorf tubes before counting in a haemocytometer. The results are summarised in Table 9 (n=l, in triplicate) , and demonstrate that the representative compound 1-24 reduces MCF-7 tumour cell proliferation, while the proliferation rate of the control group is consiεtent with the proliferation rate normally observed. The doubling rate observed for the control group is approximately 25% greater than that observed with cells exposed to compound 1-24. This indicates that comopunds of the invention are useful as anti-cancer agents. Table 9 Rate of cell growth in MCF-7 cells Example 25 Effect of Compounds of the Invention on Thvmidine Uptake Incorporation of [methyl-3H] thymidine into PNA waε measured as described by Murphy and Lazaruε (1983) , uεing MCF-7 mammary carcinoma cells treated with compound 1-24 (100/ιM) and a control group. The growth medium from cells cultured as described in Example 24 waε aεpirated from the wellε, and ImL of fresh growth medium containing lμCi [3H] -thymidine (Amersham) waε added to each well. After 4 hourε incubation, the medium was aspirated and the monolayers were washed three times with ice cold phosphate buffered saline (ICN) . This was followed by three washeε with 10% (v/v) ice cold trichloroacetic acid, each of 5 minutes duration. After another three washes with absolute ethanol at room temperature of 5 minutes duration, the monolayer was dried at room temperature for 10 minutes. The monolayer was establiεhed after incubation for 12 hours with 0.5 N NaOH and 1% Triton-X 100 (Sigma) . An aliquot of the cell lysate was added to 10ml of Ultima Gold (Packard) and counted using a liquid scintillation counter (Packard model 19006A) . Figure 5 showε the inhibition of thymidine uptake in cell populations exposed to lOOmM Glibenclamide (n=3) or Compound 1-24 (n=4) , compared to the control group (n=4) . The significant inhibition observed supports the inhibition of cell proliferation demonstrated in Example 24, and confirms the potential of compoundε of the invention to act aε anticancer agents. Thymidine uptake is a measure of PNA synthesis, which may be either associated with cell proliferation or cell repair. Agents which act to inhibit thymidine uptake normally also inhibit cell division, as demonstrated with Compound 1-24. It will be apparent to the person skilled in the art that while the invention has been described in some detail for the purposeε of clarity and understanding, various modifications and alterations to the embodiments and methods deεcribed herein may be made without departing from the scope of the inventive concept disclosed in this specification. References cited herein are listed on the following pages, and are incorporated herein by this reference. REFERENCES 1. Cohen, M.L. Prug Pevelopment Research, 1986 9_ 249-258 2. Cook, N.S., Potassium Channels. Structure, Clasεification, Function and Therapeutic Potential, Ellis Horwood Ltd, Chichester, 1990 3. Pen Hertog, H.J. and Combe, W.P. Rec. Trav. Chim. , 1951 70 581-590 4. Pen Hertog, H.J., Kolder, CR. and Combe, W.P. Rec. Trav. Chim., 1951 70 . 591-599 5. Gopalakriεhnan, M. , Janiε, R.A. and Triggle, P.J. Prug Pevelopment Research, 1993 2_8 95-127 6. Kantor, P.F., Coetzee, W.A. , Dennis, S.C. and Opie, L.H. , Circulation, 76 (Suppl. IV), 1987 17 7. Murphy, L.J. and Lazarus, L. Endocrinology, 1983 111 1026-1035 8. Palmer, K.J. and Brogden, R.N. Prugs, 1993 46 (1) 92-125 9. Robertson, P.W. and Steinberg, M.I. J. Med. Chem., 1990 3_3 (6) 1529-1541 10. Schmid-Antomarchi, H. , Amoroso, S., Fosεet, M. , and Lazdunski, M. Proc. Natl. Acad. Sci. USA, 1990 8_7 3489-3492 11. Van Ammers, M. and Pen Hertog, H.J. Rec. Trav. Chim., 1958 77 340-345 12. Woodfork, K.A., Wonderlin, W.F., Peterson, and Strobl, J.S. Journal of Cellular Physiology, 1995 162 163-171

Claims: CLAIMS 1. A εulphonyl compound of general formulae selected from the group consisting of formulae I, II, III, IV and V: III V where R represents a substituted or unsubstituted alkylaryl, , benzyl or alkyl group; X represents oxygen, sulphur, amine, or guanidine; W represents nitrogen or carbon; and Y, Q and Z independently represent hydrogen, halogen, amine, alkyl, hydroxy, alkoxy, or sulphonylurea, where each of Y, Q and Z may be the same or different, with the proviso that in formula I, when X is O, Y and Z are not both hydrogen, and in formula IV, X is not O, Y and Z are not CH 3 , Q iε not H, and R iε not C 6 H 4 CH 3 . 2. A compound according to Claim 1 in which R repreεentε an alkylaryl group; X repreεents oxygen or sulphur; W representε nitrogen or carbon; and Y, Q and Z independently represent hydrogen, halogen, amino, alkyl, hydroxy, sulphonylurea or alkoxy, where each of Y, Q and Z may be the same or different. 3. A compound according to Claim 1 or Claim 2 in which one or more of Y, Q and Z is an alkoxy group having 1 to 3 carbon atoms. 4. A compound according to any one of Claims 1 to 3 in which one or more of Y, Q or Z is a halogen selected from the group consiεting of chlorine, bromine or iodine. 5. A compound according to Claim 4, wherein one or more of Y, Q and Z iε bromine. 6. A compound according to any one of Claims 1 to 5 in which R is an alkylaryl or benzyl group, and the ring moiety of the R group is substituted with one or more chlorine, bromine, ethyl or methoxy substituents. 7. A comopund of general formula I, II, III or IV according to any one of Claims 1 to 6, in which Y, Q or Z iε εulphonylurea, and the sulphonylurea group is symmetrical with the sulphonylurea chain which is subεtituted by the R group. 8. A compound of general formula I, II or III according to any one of Claimε 1 to 7, in which the pyridine ring iε substituted by an ethoxy group. 9. A biologically-active metabolite of a compound according to any one of Claims 1 to 8. 10. A pharmaceutical composition comprising a compound according to any one of Claims 1 to 9, together with a pharmaceutically-acceptable carrier. 11. Uεe in medicine of a compound according to any one of Claimε 1 to 9. 12. A method of treatment of a condition mediated by alteration of potassium ion channel flux, comprising the step of administering an effective amount of a compound according to any one of Claims 1 to 9 to a mammal in need of such treatment. 13. A method according to Claim 12 in which the condition mediated by potasεium ion channel flux is selected from the group consisting of type II diabetes, cardiac arrhythmiaε, iεchaemic and hypoxic cardiovaεcular incidents, and cancers. 14. A process for the preparation of compounds of the general formulae I, II, III, IV and V as defined in Claim 1 which comprises the step of reacting a compound of the general formulae VI, VII, VIII or IX respectively: VI VII VIII IX wherein W, Y, Z, and Q are as defined above, with a compound of the general formula X: O II χ=C=N—S—R II O X in which X and R are as defined in Claim 1. 15. Use of a compound according to any one of Claims 1 to 9 for the manufacture of a medicament for the treatment of a condition mediated by alteration of potassium ion channel flux. 16. Use according to Claim 15, in which the condition mediated by potasεium ion channel flux iε selected from the group consisting of type II diabetes, cardiac arrhythmias, and ischaemic and hypoxic cardiovascular incidents, and cancers.

Inventors: CHAMBERS NOEL JOHN, JOHNSTON GRAHAM ALLEN ROSS, CHAMBERS, NOEL, JOHN, JOHNSTON, GRAHAM, ALLEN, ROSS
Applicants: CHAMBERS NOEL JOHN, JOHNSTON GRAHAM ALLEN ROSS, POLYCHIP PHARMACEUTICALS PTY L, UNIV SYDNEY, CHAMBERS, NOEL, JOHN, JOHNSTON, GRAHAM, ALLEN, ROSS, POLYCHIP PHARMACEUTICALS PTY. LTD., THE UNIVERSITY OF SYDNEY
Application year: 1996
Main classification: C07D213
IPC codes: C07D521