SYNTHETIC PEPTIDES AND PHARMACEUTICAL COMPOSITIONS COMPRISING THEM

Abstract: Synthetic peptides having at least fourteen amino acid residues each including two linked amino acid sequences which correspond to positions p200-208 and p262-266 of the human acetylcholine receptor alpha -subunit, but differing therefrom by one or more amino acid substitutions in each of said positions, are disclosed. These peptides inhibit the proliferative response of human peripheral blood lymphocytes to the myasthenogenic peptides p195-212 and p259-271 and are suitable for treatment of subjects afflicted with myasthenia gravis.

Description: SYNTHETIC PEPTIDES AND. PHARMACEUTICAL COMPOSITIONS COMPRISING THEM Field of the Invention The present invention relates to synthetic peptides useful for the treatment of myasthenia gravis (MG) patients, and to pharmaceutical compositions comprising these peptides by themselves, or attached to a macromolecular carrier. Description of the Background Art Autoimmune diseases are characterized by immune responses that are directed against self antigens. These responses are maintained by the persistent activation of self- reactive T lymphocytes. T lymphocytes are specifically activated upon recognition of foreign and/or self antigens as a complex with self Major Histocompatibility Complex (MHC) gene products on the ' surface of antigen-presenting cells (APC) . Myasthenia gravis (MG) is an autoimmune disorder, the symptoms of which are caused by an antibody-mediated autoimmune attack on the acetylcholine receptor (AChR) of the post- synaptic membrane of the neuromuscular junction. This antibody attack results in loss of acetylcholine receptors and jeopardizes normal neuromuscular transmission, leading to episodic muscle weakness, chiefly in muscles innervated by cranial nerves, and to fatigability. T lymphocytes are considered to play a central role in the autoreactive process, but the specific immunoregulatory mechanisms by which the T cells exert their regulatory role leading to the induction and various clinical manifestations of MG are poorly understood, and no specific cure is available for the treatment of myasthenic patients. Presently, treatment is with cholinesterase inhibitors, such as pyridostigmine and neostigmine, thymectomy, corticosteroids, and immuno- suppressive agents and plasmapheresis. MG is a well defined autoimmune disorder mediated by antibodies specific to determinants of the AChR. Specific genes of the human MHC, the HLA system, were shown to be significantly associated with the disease. The high frequency of certain histocompatibility antigens (HLA - B8, DR3) in MG patients suggests a defect of immunoregulation that might be expressed on the level of T cells. In previous studies we found that two peptides representing sequences of the human AChR α-subunit (pl95-212 and p257-269) significantly stimulated peripheral blood lymphocytes (PBL) from MG patients in comparison to healthy controls (Brocke, S. et al. (1988), J. Clin. Invest. 82:1894- 1900) . In addition, a correlation was demonstrated between the HLA-DR types of the MG patients and their responses to these peptides. Thus, all patients that expressed HLA-DR3 responded to p257-269 and 83% of patients who expressed HLA-DR5 responded to pl95-212. Extension of this research using inbred mouse strains led to the identification of high, intermediate and low responder strains to the sequences pl95-212 and p259-271 of the human AChR α-subunit. Furthermore, lymph node cells, from Torpedo-derived AChR immunized SJL and BALB/c mice, proliferated in response to pl95-212 and p259-271, respectively, even better than to the immunizing antigen (Brocke, S. et al. (1990), ___________ 69.: 95) . These results indicate that peptides pl95-212 and p259-271 are immunodominant murine T cell epitopes. Long-term T cell lines and clones of C3H.S origin specific to synthetic immunogenic peptides pl95-212 and p259- 271 were established in our laboratory and described by Brocke, S. et al. (1990a), Internat. Immunol. 2:735-742 r herein incorporated by reference. Using these cell lines and clones, it is possible to characterize the T cell recognition process of yasthenic epitopes. Using this method, T cell lines and clones specific to pl95-212 were established from lymph node cells of low (C3H.SW) and high (SJL) responder mouse strains, and T cell lines and clones specific to p259-271 were developed from lymph node cells of low (C3H.SW) and high (BALB/c) responder mouse strains. It has recently been reported that when activated cells of these cell lines are inoculated into naive syngeneic mice, peptide-specific antibodies and antibodies to the murine acetylcholine receptor are detected. In addition, decremented compound muscle action potentials (CMAP) consistent with impairment of neuromuscular transmission are seen in the line- inoculated mice. Thus, this can serve as a murine model of MG- related autoimmune manifestations. See Kirshner et al., (1994), Cell. Immunol. , 157: 11-28, the entire contents of which are hereby incorporated by reference. CMAP is also used for diagnosis of MG patients. European patent publication no. 432,691 describes an assay for the measurement of direct binding of a peptide that is a T-cell epitope to gene products of the major histocompatibility complex (MHC) , classes I and II, on the surface of intact living antigen-presenting cells (APC) . The assay comprises incubating the labelled peptide with the APC and monitoring the extent of binding by the addition of a probe that reacts with the ligand used to label the peptide. The assay is suitable for autoimmune diseases and other immunological disorders. With this assay it was demonstrated that pl95-212 binds directly to MHC class II molecules on living APC from several different mouse strains. This observed binding capacity for the peptide was shown to correlate with the proliferative potential of the different mouse strains and was inhibited by the relevant anti-I-A antibodies. In addition, it was shown that APC from MG patients and healthy controls, who responded by proliferation to peptides pl95-212 and/or p259-271, also bound the same peptides, labelled with biotin. The ability to screen peptides by their direct binding to MHC products and by their stimulatory capacity to T cells might shed light on the role of MG-related epitopes in the pathogenesis of the disease. It has been suggested that peptide analogs obtained by amino acid substitutions in a stretch of the sequence of a peptidic antigen relevant to an autoimmune disease might lead to peptides that bind to MHC gene products but that do not stimulate specific helper T cells. Such peptides can be used to inhibit competitively T cell reactivity in vitro and in vivo and thus for treatment of the corresponding autoimmune disease (Sakai, K. et al. (1989), PNAS 86:9470: Urban, J.L. et al. (1989), Cell 5__:257; European patent publication no. 432,691, and PCT publication no. -WO 92/04049. However, none of the references provide any information or guidance on the amino acids of the pathogenic peptide that can be substituted and which amino acids can serve as suitable substituents, in order to obtain a peptide analog that will successfully compete with the pathogenic peptide and thus be useful for preventing or treating autoimmune diseases in general, and myasthenia gravis in particular. Suinmary of the Invention The present invention relates to a response- inhibiting peptide of at least fourteen amino acid residues capable of inhibiting the proliferative response of T lymphocytes from a myasthenia gravis patient to a myastheno- genic peptide corresponding to a sequence of the human acetyl¬ choline receptor α-subunit selected from the group consisting of peptide pl95-212 having the formula of SEQ ID NO:l: • 195 200 205 Asp-Thr-Pro-Tyr-Leu-Asp-Ile-Thr-Tyr-His-Phe- 210 Val-Met-Gin-Arg-Leu-Pro-Leu and the peptide p259-271 having the formula of SEQ ID NO:2: 260 265 270 Val-Ile-Val-Glu-Leu-Ile-Pro-Ser-Thr-Ser-Ser-Ala-Val said response-inhibiting peptide comprising two linked amino acid sequences wherein the first amino acid sequence includes at least amino acid residues 200-208 of SEQ ID NO:l and the second amino acid sequence includes at least amino acid residues 262-266 of SEQ ID NO:2, but said sequences of the response-inhibiting peptide differing from SEQ ID NO:l and SEQ ID NO:2 by one to three amino acid substitutions in each of SEQ ID NO:l and NO:2, said substitutions being selected so as to have the function of inhibiting the proliferative response of T lymphocytes from a myasthenia gravis patient to the myasthenogenic peptides of SEQ ID NO:l and SEQ ID NO:2, said amino acid substitutions being selected from those which cumulatively do not substantially change the volume, hydrophobic-hydrophilic pattern and charge of the corresponding portions of the unsubstituted myasthenogenic peptides. The invention further relates to pharmaceutical compositions and to a method for treatment of myasthenia gravis by administration of the peptides of the invention by themselves, in a polymerized form or attached to a macromolecular carrier. Brief Description of the Drawings Figure 1 is a graph showing proliferative responses of the T cell line, TCBALB/c259-271, to various doses of the myasthenogenic peptide p259-27l and its analogs 266Asp, 262Lys and 262Asp. Figure 2 is a graph showing inhibition of the p259- 271 specific proliferative response of the T cell line TCBALB/c259-271, with various doses of the inhibitory peptides 266Asp, 262Lys, 262Asp and pl95-212. Figure 3 is a graph showing specificity of the helper activity of the T cell line, TCBALB/c259-271, with various doses of the peptides (antigen = Ag) p259-271, 266Asp, 262Lys and 262Asp. Figure 4 is a graph showing inhibition of the helper activity of the T cell line, TCBALB/c259-271, with various doses of the peptide p259-271 alone or in combination with each of the analogs 266Asp, 262Lys and 262Asp. Figure 5 is a graph showing proliferative responses of lymph node cells of BALB/c mice immunized with p259-271, to various doses of p259-271, 266Asp, 262Lys and 262Asp. Figure 6 is a graph showing inhibition of the proliferative responses of lymph node cells of BALB/c mice immunized with p259-27l, to various doses of p259-271 alone or in combination with 262Lys. Figure 7 is a graph showing inhibition of the pl95- 212 specific proliferative response of the T cell line, TCSJL195-212, with two different doses of mixtures of pl95-212 with its analog 207Ala. Figures 8A-C show the sequences and SEQ ID NOS of the analogs of pl95-212, the analogs of p259-271 and the dual epitope analogs, respectively, which were synthesized in Example 1. Figure 9 is a graph showing the results of an experiment showing the prevention of experimental autoimmune myasthenia gravis (EAMG) induction by an analog of the present invention. The plus sign (+) opposite "myasthenogenic decrement" indicates that a CMAP decrement was determined. The negative sign (-) indicates that no CMAP decrement was found. Figure 10 is a graph showing the inhibition of proliferative responses of T cell line TCSJL195-212 by the dual epitope analogs 207Ala-262Lys and 262Lys-207Ala. Figure 11 is a graph showing the inhibition of proliferative responses of T cell line TCBALB/c259-271 by the dual epitope analogs 207Ala-262Lys and 262Lys-207Ala. Figure 12 is a graph showing the in vivo inhibition of the priming of lymph node cells by the pl95-212 based analogs 207Ala, 207Ala-262Lys and 262Lys-207Ala. Figure 13 is a graph showing the in vivo inhibition of the priming of lymph node cells by the p259-271 based analogs 262Lys, 207Ala-262Lys and 262Lys-207Ala. Figure 14 is a graph showing the inhibition of lymph node cell proliferation of pl95-212 immunized SJL mice by per os administration of 262Lys-207Ala. Figure 15 is a graph showing the percent inhibition of lymph node cell proliferation of P195-212 immunized SJL mice by per os administration of 262Lys-207Ala. Figure 16 is a graph showing the percent inhibition of lymph node cell proliferation of p259-271 immunized BALB/c mice by per os administration of 262Lys-207Ala. Detailed Description of Preferred Embod—tπents The present invention relates to analogs of myasthenogenic peptides pl95-212 (SEQ ID NO:l) and p259-271 (SEQ ID NO:2) which will bind with high affinity to the appropriate MHC Class II molecules but will not lead to further activation of T cells. Examples of such analogs are provided, as is a procedure which may be followed by anyone of ordinary skill in the art in order to identify additional peptides which will also accomplish this function. The invention is based on the design and synthesis of peptides with amino acid substitutions at different positions that are based on parent peptides pl95-212 and p259-271. By means of appropriate substitutions, analogs can be identified which are antagonists to the action of the myasthenogenic epitopes in the course of myasthenia gravis. An analog which will bind with high affinity to the appropriate MHC Class II molecules but will not lead to further activation of T cells will compete with the myasthenogenic peptides which must bind to the same MHC Class II molecules in order to cause activation of the T cells which, in turn, lead to the production of the antibodies which cause myasthenia gravis. By competing with the myasthenogenic peptides which cause T cell proliferation, the adverse effects of myasthenia gravis can be ameliorated. It has been assumed that the portions of the myasthenogenic epitopes pl95-2l2 and p259-271 which are most important for their function as a T cell epitope are amino acid residues 200-208 of .SEQ ID NO:l and 262-266 of SEQ ID NO:2. Thus, the changes are preferably made in these core areas. It is expected that the amino acid residues outside of these core areas are not as important to the function of binding to the appropriate MHC Class II molecules and thus it is not expected that changes in these amino acid- residues will prevent further activation of the T cells. Accordingly, there is no need to change them, but if substitutions are made in the non-core amino acid residues, they should be selected to fulfill the guidelines discussed herein as to the cumulative effects of the substitutions. Amino acids may be divided along the lines of volume, hydrophobic-hydrophilic pattern and charge. With respect to volume, those of ordinary skill in the art understand that the amino acids with the largest volume are Trp, Tyr, Phe, Arg, Lys, lie, Leu, Met and His, while those with the smallest volumes are Gly, Ala, Ser, Asp, Thr and Pro, with others being in between. With respect to hydrophobic-hydrophilic pattern, it is well known that the amino acids Gly, Ala, Phe, Val, Leu, lie, Pro, Met and Trp are hydrophobic, whereas all of the remaining amino acids are hydrophilic. Among the hydrophilic amino acids, Ser, Thr, Gin and Tyr have no charge, while Arg, Lys, His and Asn have a positive charge and Asp and Glu have negative charges. What is important in selecting peptides to be tested for their potential in inhibiting the proliferative response of T lymphocytes from a myasthenia gravis patient to the myasthenogenic peptide SEQ ID NO:l or SEQ ID NO:2 to which it corresponds, is that the substitutions be selected from those which cumulatively do not substantially change the volume, hydrophobic-hydrophilic pattern and charge of the corresponding portion of the unsubstituted myasthenogenic peptide. Thus, a hydrophobic residue may be substituted with a hydrophilic residue, or vice-versa, as long as the total effect does not substantially change the volume, hydrophobic-hydrophilic pattern and charge of the corresponding unsubstituted myasthenogenic peptide. As indicated above, these substitutions are preferably made in the important epitopic core areas of residues 200-208 of SEQ ID NO:l and 262-266 of SEQ ID NO:2. It is preferred that the synthesized peptide of the present invention have a length of at least 14 amino acids and preferably from 25-31 amino acids. Each of the analogs preferably has from 1-3 substitutions in each core area. In a preferred embodiment of the present invention, the substitution or substitutions are selected so as to substitute a hydrophobic amino acid residue in the core area with another hydrophobic amino acid residue selected so as not to cumulatively substantially change the volume of the unsubstituted peptide. For example, in p200-208, thε hydrophobic amino acids are 201 lie, 205 Phe, 206 Val and 207 Met. Each of these may be substituted with any of the other hydrophobic residues in this preferred embodiment. Thus, for example, 207 Met may be substituted with Gly, Ala, Phe, Val, Leu, lie, Pro or Trp. It has been established that substitution with Ala provides particularly good results. Similarly, in p262-266, 263 Leu, 264 lie and 265 Pro are all hydrophobic. Thus, from one to three of these can be substituted with another hydrophobic residue so long as the cumulative effect on the volume is not substantial. Thus, for example, the 265 Pro may be substituted by Gly, Ala, Phe, Val, Leu, lie, Met or Trp. In a second embodiment, a hydrophilic non-charged residue may be replaced by a charged or non-charged hydrophilic residue. Hydrophilic non-charged residues in the p200-208 peptide include 202 Thr, 203 Tyr and 208 Gin. In the p262-266 peptide, 266 Ser is a non-charged hydrophilic residue. Thus, for example, 208 Gin may be changed to any of Ser, Thr, Tyr, Arg, Lys, His, Asn, Asp or Glu and 266 Ser may be changed to any Thr, Gin, Tyr, Arg, Lys, His, Asn, Asp or Glu. Again, the change must be selected such that the cumulative effect of all changes does not substantially change the volume, hydrophobic- hydrophilic pattern and charge of the corresponding portion of the unsubstituted myasthenogenic peptide. Specific examples of changes within this embodiment are the substitution of 208 Gin with Asn or Asp and the substitution of 266 Ser with Lys or Asp. In another embodiment of the present invention, charged hydrophilic residues are substituted by charged (same or opposite charge) or non-charged hydrophilic residues. In the p200-208 peptide, 200 Asp is a negatively-charged hydrophilic residue and 204 His is a positively-charged hydrophilic residue. In p262-266, 262 Glu is a negatively- charged hydrophilic residue. Thus, for example, 200 Asp may be substituted with Ser, Thr, Gin, Tyr, Arg, Lys, His, Asn or Glu and 262 Glu may be substituted with Ser, Thr, Gin, Tyr, Arg, Lys, His, Asn or Asp. Specific examples may be substituting 200 Asp with Lys (opposite charge) or 262 Glu with Ser (charged to non-charged) . It has been found that substituting 262 Glu with Lys (negative charge to positive charge) gives particularly good results. Again, whatever substitution is made must be selected so as to not cumulatively substantially change the volume, hydrophobic-hydrophilic pattern and charge of the corresponding portion of the unsubstituted myasthenogenic peptide. Finally, in a further embodiment, it is also possible tc replace a hydrophilic residue with a hydrophobic residue or vice-versa, as long as the cumulative effect is within the guidelines. Examples of such substitutions are the substitution of 204 His with Gly or 203 Tyr with Phe or the substitution of 262 Glu with Ala. Those of ordinary skill in the art of peptide chemistry will readily recognize, by strictly theoretical considerations, what the cumulative effect will be on the charge, hydrophobic-hydrophilic pattern and volume of a given peptide as short as nine amino acids. Thus, it would not take undue experimentation to determine which substitutions should be tried in order to identify analogs in accordance with the present invention which have the function of competing with the native myasthenogenic peptides for binding the appropriate MHC Class II molecules but which will- not lead to further activation of T cells. It should be understood that the amino acids which are used for substituting into the native peptide may include modified peptides such as norleucine, hydroxyproline, hydroxylysine, gamma-carboxyglutamic acid, etc. Some specific substitutions, any 1-3 of which which otherwise comply with the guidelines presented herein, may be present in an analog according to the present invention, include the following: 200, Asp-->Lys; 203, Tyr-->Phe; 204, His-->Gly; 207, Met-->NLeu; 207, Met-->Ala; 208, Gln-->Asp; 208, Gln-->Asn; 262, Glu-->Asp; 262, Glu-->Lys; 262, Glu-->Ser; 262, Glu--Ala; 265, Pro-->Leu; 265, Pro-->Phe; 266, Ser-->Lys; and 266, Ser-->Asp. The nomenclature of these analogs when including the entire pl95-2l2 or p259-272 sequence except for one to three changes will hereinafter be by identification of the position and substituted residue only. The most preferred analogs are 204Gly, 207Ala, 262Lys, 262Ser, 265Leu and 265Phe, i.e., SEQ ID NOS: 5, 6, 11, 12, 14 and 15, respectively. It is expected that the substitution of hydrophilic amino acids, that are mainly involved in T cell receptor interactions, will be effective in blocking T cell activation. Substitutions in the hydrophobic residues may contribute to higher stability of the analog-MHC complexes. It should be understood that other modifications of the myasthenogenic peptides are also contemplated by the present invention. Thus, the peptide of the present invention is intended to include a "chemical derivative" thereof which retains at least a portion of the function of the peptide which permits its utility in preventing or inhibiting T cell proliferative responses and autoimmune disease. A "chemical derivative" of a peptide of the present invention contains additional chemical moieties not normally a part of the peptide. Covalent modifications of the peptide are included within the scope of this invention. Such modifications may be introduced into the molecule by reacting targeted amino acid residues of the peptide with an organic derivatizing agent that is capable of reacting with selected side chains or terminal residues. Many such chemical derivatives and methods for making them are well known in the art. Also included in the scope of the invention are salts of the peptides of the invention. As used herein, the term "salts" refers to both salts of carboxyl groups and to acid addition salts of amino groups of the peptide molecule. Salts of a carboxyl group may be formed by means known in the art and include inorganic salts, for example, sodium, calcium, ammonium, ferric or zinc salts, and the like, and salts with organic bases such as those formed for example, with amines, such as triethanolamine, arginine, or lysine, piperidine, procaine, and the like. Acid addition salts include, for example, salts with mineral acids such as, for example, hydrochloric acid or sulfuric acid, and salts with organic acids, such as, for example, acetic acid or oxalic acid. Such chemical derivations would preferably be used to modify the pharmaceutical properties of the peptide insofar as stability, solubility, etc., are concerned. Once an analog in accordance with the present invention is produced, its ability to inhibit the proliferative response of T lymphocytes to the corresponding myasthenogenic peptides may be readily determined by those of ordinary skill in the art without undue experimentation using tests such as those described herein. One test which may be readily conducted is for the ability of substituted peptides to inhibit in vi tro the proliferative responses of certain T cell lines and clones to the original peptide. The T cell lines and clones are those which have been produced which are specific to the myasthenogenic epitopes of SEQ ID NO:l or SEQ ID NO:2. See Brocke et al. (1990a) and Kirshner et al. (1994), supra . Another test which can be conducted in order to select analogs having the desired activity is to test for the ability of the substituted peptides to inhibit the ability of the T cell lines and clones to provide help to peptide-specific B cells in the presence of the parent peptide. The substituted peptides may also be tested for their ability to bind directly, following biotinylation, to MHC Class II products on antigen-presenting cells of the relevant strains and to inhibit the binding of the parent myasthenogenic epitopes. Substituted peptides which test positive in one or more of these in vitro tests will provide a reasonable expectation of in vivo activity. However, in vivo tests can also be conducted without undue experimentation. One such in vivo animal test would be to immunize naive mice with the myasthenogenic peptide and co-administer the mice with the analogs by various routes and dose schedules. Lymph node cells can then be analyzed for their proliferative potential to the myasthenogenic peptides. In addition, titers of anti-AChR antibodies in the sera of these mice can be measured to record the effect of the analogs on the in vivo helper cell activity. The advantages of these in vivo assays is that, although they are not aimed at assessing the effects of the analogs on disease induction, they allow in vivo screening of all analogs in a relatively short time. As final proof of therapeutic activity, such activity may be directly measured in a murine model in vivo. It has previously been shown that some T cell lines arid clones specific to the myasthenogenic T cell epitopes are capable of inducing MG-related autoimmune manifestations in mice (Mozes et al. (1991), "Abstracts", 15th International Congress of Biochemistry, Jerusalem, p.20 and Kirshner et al. (1994), supra) . Therefore, naive mice can be injected with such clones and treated with the selected substitute peptides in order either to prevent or to remit the autoimmune responses. The peptides can be injected into the mice by different routes at different dosages and at different time schedules. In order to determine the pharmacokinetic parameters of the analogs, including volume " of distribution, uptake into antigen- presenting cells and clearance, one can use biotinylated derivatives of the analogs. The ^ concentration of the soluble fraction of the analogs in the various body fluids can be determined by ELISA, using avidin-coated plates and specific anti-peptide antibodies. Cell bound analogs can be analyzed by FACS, using fluorochrome-conjugated avidin or streptavidin. Furthermore, the treated mice can be tested periodically in order to determine the effect of the peptides on the autoimmune responses and on disease manifestations elicited in the mice by the T cell clones. It can thus be seen that, besides the preferred embodiments which have been shown to be operable in the examples herein, those of ordinary skill in the art will be able to determine additional analogs which will also be operable following the guidelines presented herein without undue experimentation. A relatively simple in vitro test can also be conducted in order to assay for the expected therapeutic efficacy of any given substituted peptide on any given myasthenia gravis patient. In order to assess the ultimate goal of producing peptides that will bind with high affinity to the appropriate MHC Class II molecules but will not lead to further activation of T cells and will therefore have a therapeutic effect on MG patients, the peptides may be assayed, following biotinylation, for their ability to bind directly to HLA Class II products on antigen-presenting cells in the peripheral blood lymphocytes of the myasthenia gravis patients and to inhibit the binding of the parent myasthenogenic epitopes. Healthy control donors and control peptides may be used in such assays to verify their specificity. A preferred form of the therapeutic agent in accordance with the present invention is the form of a ulti- epitope single peptide. Thus, in a preferred embodiment, analogs of each of the two myasthenogenic peptides are covalently linked to one another, such as by synthesizing them, either by chemical or biotechnological means, directly adjacent one another as a single peptide, or connecting them by means of a short stretch of alanine residues or by a putative site for proteolysis by cathepsin. See, for example, U.S. Patent 5,126,249 and European Patent 495,049 with respect to such sites. This will induce site-specific proteolysis of the preferred form into the two desired analogs. The dual tandem analogs can have substantially the same activity as each of the analogs which are part of them and therefore have a therepeutic potential that will be capable of inhibiting T cell responses from a wide range of MG patients. Alternatively, a number of the same or different substituted peptides of the present invention may be formed into a peptide polymer such as, for example, polymerization of the peptides with a suitable polymerization agent, such as 0.1% glutaraldehyde (Audibert et al. (1981), Nature 289:593) . The polymer will preferably contain from 5 to 20 peptide residues. Such peptide polymers may also be formed by cross-linking the peptides or attaching multiple peptides to macromolecular carriers. Furthermore, the formulation may simply be a mixture of different peptides in accordance with the present invention. Suitable macromolecular carriers are, for example, proteins, such as tetanus toxoid, and linear or branched copolymers of amino acids, such as a linear copolymer of L- alanine, L-glutamic acid and L-lysine and a branched copolymer of L-tyrosine, L-glutamic acid, L-alanine and L-lysine (T,G)-A- -L, or multichain poly-DL-alanine (M. Sela (1969) , Science 166: :1365-1374) . The conjugates with the carriers are obtained, for example, by first coupling the peptide with a water-soluble carbodiimide, such as 1-ethyl-3 (3 ' -dimethylaminopropyl) carbodiimide hydrochloride, and then performing the conjugation with the macromolecular carrier as described by Langbeheim et al. (1976), Proc. Natl. Acad. Sci. USA 73:4636-4640. The contents of the coupled peptide in each conjugate are determined by amino acid analysis, in comparison to the composition of the carrier alone. According to a preferred embodiment of the present invention, one or more active peptides may be attached to a suitable macromolecular carrier or may be polymerized in the presence of glutaraldehyde. In a preferred embodiment of the present invention, the 207Ala and 262Lys were synthesized in tandem without chemical linkers or link sequences. Two peptides were synthesized, one in which the N-terminal sequence was that of 207Ala and the C-terminal sequence was that of 262Lys (designated 207Ala-262Lys (SEQ ID NO: 17)), and another in which the order of the analogs was reversed (designated 262Lys- 207Ala (SEQ ID NO: 18)). Neither of the dual epitope analogs stimulated the proliferative responses of either the pl95-212 or the p259-271 specific T cell lines. On the other hand, both dual analog peptides inhibited significantly the proliferative response of the latter lines to the myasthenogenic epitopes. Furthermore, both dual analogs were capable of inhibiting the in vivo priming of lymph node cells to either myasthenogenic epitope. It appeared that the dual analog 262Lys-207Ala was more efficient in inhibiting pl95-212 specific responses than analog 207Ala-262Lys. No significant differences could be observed in the efficacy of inhibition of the p259-271 specific responses by the two dual analogs. The inhibitory capacity of thε two dual peptide analogs was tested on PBL of MG patients. The results demonstrated that the response to pl95-2l2 of PBL of 21 out of 22 responder MG patients was inhibited by either dual analog. Similarly, PBL of 14 out of 16 MG patients that responded to p259-27l were inhibited by either 262Lys-207Ala or 207Ala-262Lys. Thus, both dual analogs appeared to be effective in inhibiting the responses of PBL of almost all MG patients tested to the myasthenogenic epitopes. The peptides, polymers thereof or their conjugates with suitable macromolecular carriers, will be given to patients in a form that insures their bioavailability, making them suitable for treatment. If more than one peptide analog is found to have significant inhibitory activity, these analogs will be given to patients in a formulation containing a mixture of the peptides or as a single peptide in which both analogs are synthesized in tandem. If an individual patient responds to both pathogenic MG related peptides, namely pl95-212 and p259-271, the ultimate treatment will contain the appropriate inhibitory analogs of both peptides in a suitable form. The invention further includes pharmaceutical compositions comprising at least one synthetic peptide according to the invention, either single, tandem or multiple, a conjugate thereof with a suitable macromolecular carrier or a polymer thereof optionally with a pharmaceutically acceptable carrier. The route of administration may include oral, intravenous, subcutaneous, intraarticular, intramuscular, by inhalation, intraperitoneal, intranasal, intrathecal, intradermal, transdermal or other known routes, including the enteral route. In vivo tests have established the efficacy of oral administration. The dose ranges for the administration of the compositions of the present invention are those large enough to produce the desired effect, whereby, for example, an immune response to the myasthenogenic peptide, as measured by T cell proliferation in vitro, is substantially prevented or inhibited, and further, where the disease is significantly treated. The doses should not be so large as to cause adverse side effects, such as unwanted cross reactions, generalized immunosuppression, anaphylactic reactions and the like. Effective doses of the peptides of this invention for use in treating an immune-related disease are in the range of about 1 μg to 100 mg/kg body weight. The dosage administered will be dependent upon the age, sex, health, and weight of the recipient, kind of concurrent treatment, if any, frequency of treatment, and the nature of the effect desired. The synthetic analogs of sequences of the human AChR are aimed at inhibiting or suppressing specific antigen responses of MG patients, without harming other immune responses. This approach is of utmost importance since the currently accepted treatment for MG involves administration of immunosuppressive agents that are both non-specific and have multiple adverse side effects. The invention will now be illustrated by the following non-limiting examples. EXAMPLES Example 1. Preparation of the peptides The synthetic peptides pl95-212 and p259-271 and their analogs 200Lys; 203Phe; 204Gly; 207Ala; 208Asp; 208Asn; 209Lys; 262Asp; 262Lys; 262Ser; 265Ala; 265Leu; 265Phe; and 266Asp are synthesized by the Mεrrifield solid phase technique (Merrifield et al. (1963), J. Am. Chem. Soc. 8.5:2149) , with a peptide synthesizer, using commercially available side-chain protected amino acids. Amino acids are added at each step with at least 99% efficiency. The prαtecting groups are removed and the peptides are cleared from the resin with anhydrous HF. The peptides are purified by extraction with ethyl acetate or isopropyl acetate and by HPLC. The purity of the peptides is verified by HPLC and by amino acid analysis. The sequences and SEQ ID NOs of each of the above analogs are shown in Figures 8A and 8B. While all of the above peptides have only one substitution each, other peptides having two or three substitutions and retaining the goals of the guidelines presented herein may be synthesized in the same manner. Also synthesized in the same manner were two dual analogs, one in which the N-terminal sequence was that of 207Ala and the C-terminal sequence that of 262Lys (designated 207Ala-262Lys) and another in which the order of the analogs was reversed (designated 262Lys-207Ala) . The sequences of these dual analogs and their SEQ ID NOs are shown in Fig. 8C. Example 2. Inhibition of proliferative responses in vitro of T cell clones to peptide p259-27l T cell lines and clones specific to p259-271 were developed from lymph node cells of high responder BALB/c mice according to the method described by Brocke et al. (1990a), supra, and designated TCBALB/c259-27l (Mozes, E. et al. (1991), supra; see also Kirshner et al. (1994), supra ) . The proliferative response of the T cell clones was assessed by measuring 3 H-thymidine incorporation into cells following incubation for the final 16 hours of culture. Cells (10* cells/well) of the T cell line TCBALB/c259-271 were incubated in the presence of irradiated (3000 rad) syngeneic spleen cells from BALB/c mice (0.5 x 10 s cells/well) as antigen-presenting cells, in the presence of various concentrations (1, 5, 10, 20 μg/well) of the peptides pl95-212, p259-271, 266Asp, 262Lys and 262Asc. Cultures were set in 0.2 ml RPMI 1640 medium supplemented with 2mM glutamine, ImM sodium pyruvate, non-essential amino acids, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml fungizone, 5 x 10 " 5 M 2- mercaptoethanol, lOmM HEPES buffer (enriched medium) and 10% fetal calf serum (FCS) , for 48 hr, followed by an overnight pulse with 3 H-thymidine (0.5 μCi of 5 Ci/mmol). Following incorporation of the isotope, cells were harvested 16 hr later onto a filter paper and radioactivity was determined. The results are shown in Fig. 1 (expressed as mean CPM of triplicates ± SD) . Peptides 266Asp and 262Asp triggered low proliferative responses of the T cell line at all doses tested (up to 12% and 34.3% respectively, of the response obtained with P259-271), whereas 262Lys did not stimulate the TCBALB/c259-271 cell line to proliferate. Inhibition of the proliferative responses was performed by addition of increasing doses of the tested substituted peptides (25, 50, 75, 100 μg/well) into the in vitro proliferative cultures. Cells (10 A cells/well) of the T cell line TCBALB/c259-271, were incubated in the presence of irradiated syngeneic spleen cells (0.5 x 10 6 cells/well), p259- 271 (1 μg/well) and the various doses of the inhibitory peptides 266Asp, 262Lsy and 262Asp and of pl95-212, for 48 h. Thereafter, 3 H-thymidine (0.5 μCi of 5Ci/mmol) was added, and 16 h later plates were harvested onto a filter paper. Results are expressed as % of inhibition of the p259-271 specific proliferative response. As shown in Figure 2, 262Lys inhibited up to 93% of the proliferative response of the TCBALB/c259-271 line to p259-271. It is not surprising that peptide pl95-212 is also shown to competitively inhibit proliferation of T cells specific for peptide p259-271, as pl95-212 is directed to the same MHC-determinant as p259-271. Brocke et al. (1990a), supra, reports that pl95-212 inhibits the proliferative response of the TCSW 259-271 T cell line and p259-271 inhibits the specific proliferative response of the TCSW 195-212 line. Similar tests for the proliferative response of the other synthesized analogs were also conducted. The results with respect to all of these analogs are shown in Table 1. "Stimulators" are analogs that stimulated T cells; "inhibitors" are analogs that could not induce stimulation but could inhibit the stimulating activity of the myasthenogenic peptides; and "no effect" refers to analogs that neither stimulated nor inhibited T cells specific to the myasthenogenic peptides. At least for 203Phe analog of pl95-212, direct binding measurements revealed its ability to bind to antigen-presenting cells of SJL strain mice, in which pl95-212 is immunodominant. Example 3. Jn vitro Antibody Production Assay The synthetic peptides 266Asp, 262Lys and 262Asp were examined for their ability to stimulate the TCBALB/c259- 271 T cell line to collaborate with peptide-specific B cells in an antibody production assay. B cells, purified from spleens of BALB/c mice previously immunized with 20 μg of p259-271, were cultured (0.5 x 10 6 cells/well) with normal syngeneic spleen cells (0.5 x 10 6 cells/well), different doses of 266Asp, 262Lys and 262Asp (0.001, 0.01, 0.1, 1 and 5 μg/well) and cells of the TCBALB/c259-271 line (10 A cells/well), in 96-well microtiter plates. After 3 days incubation period, medium (enriched RPMI supplemented with 7.5% FCS) was exchanged with fresh medium without antigen, and 4 days later, supernatants were harvested and tested (by solid phase RIA) for the presence of specific anti-p259-271 antibodies. Results are expressed as mean CPM of triplicates ± SD. As can be seen in Figure 3, low antibody titers were obtained in the presence of 266Asp, whereas the helper activity stimulated by 262Asp was as efficient as that observed in the presence of the parent peptide. In contrast, no antibody activity could be detected when 262Lys was added to the culture. Inhibition of the T cell helper activity was performed by addition of increasing doses of the tested substituted peptides into the in vitro culture mixtures. B cells, purified from spleens of BALB/c mice previously immunized with 20 μg of p259-271, were cultured (0.5 x 10 6 cells/well) with normal syngeneic spleen cells (0.5 x 10 6 cells/well), different doses of p259-27l alone (0.001, 0.01, 0.1, 1 and 5 μg/well) or together with 266Asp, 262Lys or 262Asp ( 20μg of each) and cells of the TC3ALB/c259-271 line (10* cells/well), in 96-well microtiter plates. After 3 days incubation period, medium as above was exchanged with fresh medium, and 4 days later, supernatants were harvested and tested (by solid phase RIA) for the presence of specific anti- p259-271 antibodies. Results are expressed as mean CPM of triplicates ± SD. As shown in Figure 4, p259-271 specific antibody production was inhibited up to 80% in the presence of 262Lys. Example 4. Antigen-Specific Proliferative Response of Mouse Lymph Node Cells After Immunization with P259-271 In order to find out whether the peptides 266Asp, 262Lys and 262Asp will either stimulate or inhibit the proliferative response of a more hetεrogeneous T cell population, namely lymph nodes, the following experiments were performed. The peptides 266Asp, 262Lys and 262Asp were examined for their ability to stimulate lymph node cells of p259-271 immunized BALB/c mice to proliferate, in comparison to the parent peptide p259-271. Lymph node cells (0.5 x 10 6 cells/well) obtained from BALB/c mice immunized with p259-271, were incubated in enriched medium containing 1% normal mouse serum, in the presence of various concentrations of the peptides (10, 20, 50, 100 μg/well) for 96 h. Thereafter, 3 H- thymidine (0.5 μCi of 5 Ci/mmol) was added and 16 h later plates were harvested onto a filter paper. Results are expressed as mean CPM of triplicates. As shown in Fig. 5, peptides 266Asp and 262Asp triggered low proliferative responses of the cells (up to 53.2% and 32.3% respectively, of that obtained by using p259-271) whereas 262Lys did not stimulate the lymph node cells to proliferate. Inhibition of the proliferative responses of lymph node cells of BALB/c mice immunized with p259-271 was done by adding the peptide analog 262Lys into the incubation mixture at the same time as the pathogenic peptide p259-271. Lymph node cells (0.5 x 10 6 cells/well) obtained from BALB/c mice immunized with p259-271, were incubated in the presence of various concentrations of p259-271 (1, 5, 10, 20 μg/well) and 100 μg/well of 262Lys for 96 h. Thereafter, 3 H-thymidine (0.5 μCi of 5 Ci/mmol) was added and 16 h later plates were harvested onto a filter paper. Results are expressed as mean CPM of triplicates. As shown in Fig. 6, the peptide 262Lys inhibited up to 64.7% of the proliferative response of the lymph node cells to p259-271. Example 5. Tn ibi ion of Proliferative Responses in vitro of T Cell Clones to Peptide p!95-212 T cell lines and clones specific to pl95-212 were established from lymph node cells of high (SJL) responder mouse strain by the method described by Brocke et al. (1990a), supra, and designated TCSJL195-212 line (Mozes, E. et al. (1991), supra; see also Kirshner et al. (1994), supra ) . The proliferative response of the T cell clones was assessed as in the protocol Example 2 which resulted in Fig. 2. The pl95-212 peptide analog, 207Ala, did not stimulate cells of the TCSJL195-212 line to proliferate. Moreover, as shown in Figure 7, 207Ala inhibited more than 99% of the proliferative response of the TCSJL195-212 line to pl95- 212. This represents very substantial inhibition. Example 6. Inhibition of Proliferation of Human T Cells to the Pathogenic Peptides of Myasthenia Gravis The action of the pathogenic peptide p259-271 and its analogs 266Asp, 262Lys and 262Asp, and of pl95-212 and its analog 207Ala, on proliferative responses of human T cells was assessed. Peripheral blood lymphocytes (PBL) of myasthenia gravis patients and of the appropriate control donors (2 x 10 s cells/well) were assayed in microtiter plates in 0.2 ml enriched medium containing 10% autologous serum, in the presence of different doses of the peptides pl95-212 and the analog 207Ala for 96h, followed by an overnight pulse with 0.5 μCi of 3 H-thymidine. Cells were harvested and radioactivity determined. Inhibition of the proliferative responses was done by adding- different doses of 207Ala into the incubation mixture at the same time as the pathogenic peptide pl95-212. Results are reported in Table 2 as (SI) stimulation indices (col. 2 and 3), % inhibition (col. 4) . The PBL of MG patients were tested for their ability to proliferate in the presence of pl95-2l2 and the peptide analog 207Ala. Further, the ability of the analog 207Ala to inhibit pl95-212 specific proliferative responses of the PEL was tested. Table 2 summarizes the responses of three MG patients in these tests. As can be seen in this table, PBL from patients E.K., C.G. and M.R. proliferated in response to pl95-2l2 (SI=6.3, 4.3 and 3.6, respectively), whereas no proliferative responses to the analog were detected at all concentrations (10, 25, 50, 100 μM, corresponding to 20, 50, 100, 200 μg/well) tested. In addition, the analog was able to inhibit the proliferative responses of the PBL from all three patients by 77-100% at an Inhibitor:Stimulator (I:S) ratio of 1:1. TABLE 2 Peripheral Blood Lymphocyte Proliferative Responses of Myasthenia Gravis Patients to pl95-212 and its Analog 207Ala * Stimulation Index = response/background t Concentration of peptide at SI The PBL of MG patients were assayed as above for their ability to proliferate in the presence of different doses of p259-271 and its analogs 266Asp, 262Lys and 262Asp. In addition, the ability of different doses of the analogs to inhibit p259-271 specific proliferative responses of the PBL was tested. Table 3 summarizes the responses of three MG patients in these tests. As can be seen in this table, PBL from patients I.T., E.K. and C.G. proliferated in response to p259-271 (SI=4.1, 3.7 and 2.7, respectively). Moreover, for each patient, at least one analog was able to inhibit the p259- 271 induced proliferative response of the PBL. TABLB 3 Peripheral Blood Lymphocyte Response of Myasthenia Gravis Patients to p259-271 and its Analogs Patient p259-271 266Asp 262Lys 262Asp SI* % Inhibition % Inhibition % Inhibition (ug/well)t (I:S)** (I:S) (I:S) I.T. 4.1 (25) 34.3 (4:1) 0 78 (1:1) E.K. 3.7 (100) 82.7 (1:1) 100 (1:1) 70 (1:1) C.G. 2.7 (100) 85.6 (1:1) 71.6 (1:1) 65 (1:1) * Stimulation Index = response/background t Concentration of peptide at SI ** Inhibitory peptidestimulating peptide A larger experiment on 40 MG patients has also been conducted using analogs 207Ala, 204Gly / 262Lys and 262Ser. Out of 40 MG patients that were tested for this purpose 25 (63%) responded to pl95-212 and 23 (58%) responded to p259-271. Seventy eight percent of the patients responded to either or both myasthenogenic peptides. Analog 207Ala inhibited the response of PBL of -23 out of 24 (96%) responder MG patients and peptide analog 204Gly inhibited the response of PBL of 24 (100%) MG patients to pl95-212. Similarly, the responses to p259-271 of PBL of 19 and 18 MG patients out of 20 tested were inhibited by analogs 262Lys and 262Ser, respectively. Thus, the peptide analogs are very efficient in inhibiting the responses of PBL of almost all responder MG patients to the myasthenogenic epitopes. The inhibitory capacity of the two dual peptide analogs was also tested on PBL of MG patients. The results demonstrated that the response to pl95-212 of PBL of 21 out of 22 responder MG patients was inhibited by either dual analog. Similarly, PBL of 14 out of 16 MG patients that responded to p259-27l were inhibited by either 262Lys-207Ala or 207Ala-262Lys. Thus, both dual analogs appeared to be effective in inhibiting the responses of PBL of almost all MG patients tested to the myasthenogenic epitopes. The results of these tests are shown in Tables 4 and 5. TABLE 4 Inhibitory Capacity of pl95-212 Based Analogs on Stimulation of PBL of MG Patients in the Presence of pl95-2l2 Inhibitory % Inhibition Analog activity Mean + SD Range 204Gly 100% (24/24) 83.2 ± 22.5 23-100 207Ala 96% (23/24) 80.5 ± 21.9 35-100 207Ala-262Lys 95% (21/22) 89.9 ± 18.9 40-100 262Lys-207Ala 95% (21/22) 97.6 ± 5.7 79-100 TABLE 5 Inhibitory Capacity of p259-271 Based Analogs on Stimulation of PBL of MG Patients in the Presence of p259-271 Inhibitory % Inhibition Analog activity Mean + SD Range 262Lys 95% (19/20) 91.8 ± 15.8 39-100 262Ser 90% (18/20) 88.2 ± 14.9 57-100 207Ala-262Lys 94% (16/17) 84.6 ± 21.3 36-100 262Lys-207Ala 88% (15/17) 90.1 ± 21.4 23-100 Example 7. Induction and Treatment of Experimental MG in Mice As described above (Mozes (1991) and Kirshner (1994)), both pl95-212 specific T cell line of SJL origin and p259-27l T cell line of BALB/c origin, are capable of initiating MG-related manifestations upon inoculation into naive syngeneic mice. Such MG-related manifestations have been demonstrated by the production of autoantibodies to self (murine) AChR and by the determination of compound muscle action potential (CMAP) decrement. This is accomplished by inoculating naive syngeneic mice (i.v. in PBS 1 to 4 times) with activated peptide specific T cells of the lines TCSJL195- 212 and TCBALB/c259-271 or their derived clones (5-10 x 10 6 cells) . To find out whether the peptide analogs are capable of inhibiting the induction of experimental MG, a first group of 10 BALB/c mice was injected with cells of the p259-271 specific line while a second group of 10 BALB/c mice was injected with the T cell line concomitant with 200 μg of analog 262Lys. Whereas in 5 of the 10 mice injected with the ' T cell line CMAP decrements could be determined, in only 1 of the 10 mice that were treated with the peptide analog could a CMAP decrement be detected. In none of the 13 control mice CMAP decrements could be determined. Thus, analog 262Lys is capable of inhibiting the clinical manifestations of experimental MG. The results of this experiment are graphed in Fig. 9. Example 8. Binding of Analogs to MHC Since the aim of the present invention is to design peptide analogs that will bind efficiently to MHC class II on antigen presenting cells but will not stimulate the specific T cells for proliferation, the binding capacity of the peptide analogs was compared to that of the myasthenogenic peptides. The binding of the biotinylated peptide analogs 207Ala, 204Gly, 207Ala-262Lys and 262Lys-207Ala to MHC class II on murine and human antigen presenting cells has been analysed. Biotinylation of neotide N-Terminal biotinylation of peptide pl95-212 and its derived analogs, including the dual analog 207Ala-262Lys, was performed in 0.1N sodium bicarbonate solution at 0°C with a two fold molar excess of biotin-N-hydroxysuccinimide (Sigma Chemical Co., St. Louis, MO) dissolved in dimethylsulfoxide (5 mg/ml) . N-terminal biotinylation of p259-271 and its derived analogs, including the dual analog 262Lys-207Ala, was performed in 0.1N sodium bicarbonate solution at room temperature, with a two fold molar excess of biotinamidocaproate N-hydroxysuc- cinimide ester (Sigma) dissolved in 1-methyl-2-pyrrolidone (5 mg/ml) . The addition of a caproyl spacer between the biotin moiety and Val 59 of p259-271-based peptides was necessary to allow the detection of MHC-peptide interactions by streptavidin (Zisman et al., Internat. Immunol.. 6:683 (1994)). Direct binding of biotinylated peptides to APC of human subjects APC of patients with the autoimmune disease MG and of healthy donors were collected from the Ficoll fraction following Ficoll-Hypaque density centrifugation (Pharmacia Fine Chemicals, Uppsala, Sweden) . The cells in this fraction were mainly monocytes and granulocytes with less than 10% lymphocytes, as determined by direct staining with FITC conjugated- anti-CD14, anti-CD3 and anti-B220 (Sigma) , and were used as APC in the binding experiments. Alternatively, PBL were isolated from the whole blood by Ficoll-Hypaque density centrifugation, washed, suspended in 10% fetal calf serum (FCS) in RPMI 1640, and incubated in Petri dishes (5xl0 7 cells/5 ml/dish, Nunc, Roskilde, Denmark) at 37° C for 1 hr. Thereafter non-adherent cells were removed and the plates were washed three times with RPMI and placed on ice. Adherent cells were collected using a rubber policeman, and were used as APC in the binding experiments. The cells in this fraction also contained less than 10% lymphocytes, as determined by direct staining with FITC conjugated- anti-CD14, anti-CD3 and anti-B220. The APC were washed twice with a solution of phosphate buffered saline (PBS, pH=7.4) containing 0.1% bovine serum albumin (BSA, high purity grade, Amresco, OH, further referred to as PBS/BSA) , and were incubated (1x10 s /sample) for 20 hr with the biotinylated peptides or with PBS/BSA alone, in a 37°C incubator containing 5% C0 2 , followed by incuation with phycoerythrin (PE) -conjugated streptavidin (1:40, Jackson Immunoresearch, West Grove, PA) at 4°C for 30 min as described for murine splenic adherent cells (Zisman et al. (1994) supra; Mozes et al., EMBO J.. 8:4049 (1989)). After each incubation the cells were washed twice at 4°C with the above solution. To amplify the fluorescence signal an extended staining procedure was used. In this protocol, the incubation with PE-streptavidin was followed by incubations with biotinylated anti-streptavidin (1:60, Vector Laboratories, Burlingame, CA, USA) and a second incubation with PE-streptavidin, both at 4°C for 30 min. Thereafter, the cells were analyzed by flow cytometry using the FACScan cytometer and lysis software or the FACSort cytometer and CELLQuest software (Becton-Dickinson, Mountain View, CA, USA). In each analysis minimally 5,000 cells were examined. Dead cells were excluded on the basis of the forward- and side-angle light scatter. Binding data were presented as MFI, mean fluorescence intensity of the cells in the presence or absence of the biotinylated peptide. Alternatively, percentage of bound cells was calculated and presented as net values above the background signal of cells that were incubated in the absence of biotinylated peptides. In a similar manner binding of the biotinylated peptide to MHC class II of SJL mice was determined. Results For both species, the binding of analog 207Ala to MHC class II was better than that of analog 204Gly and at least as good as that of the myasthenogenic epitope pl95-212. The affinity of the binding of the dual analog 262Lys-207Ala to MHC class II of SJL mice has been calculated to-be 10-fold higher than that of its counterpart dual analog 207Ala-262Lys. Furthermore, the pattern of the binding of the dual analog 262Lys-207Lys is more homogeneous than that of the myasthenogenic peptide pl95-212. These results indicate that the dual epitope analogs are of therapeutic value. Example 9. Inhibition of Proliferative Responses by the Dual Epitope Analogs Cells (10* per well) of the T cell line TCSJL195-212 or TCBALB/c259-271 were incubated in the presence of irradiated syngeneic spleen cells (0.5 x 10 6 ) , either pl95-212 or p259-271 (lOμM) and various concentrations of the dual epitope analogs 207Ala-262Lys or 262Lys-207Ala for 48 h. Thereafter, 3 H- thymidine was added and 16 h later plates were harvested. The results, expressed as % inhibition of the pl95-212 or p259-27l specific proliferative response, are shown in Figs. 10 and 11. Inhibition of the non-specific proliferative response of the TCBALB/c259-271 line to Con-A sup was also tested. 207Ala-262Lys inhibited this proliferative response up to 67.8% at a concentration of lOOmM or up to 73% at 150mM. 262Lys- 207Ala did not inhibit this proliferation non-specifically. These results indicate that the dual analog 262Lys-207Ala inhibits the proliferative responses of both the TCSJL195-212 and TCBALB/c259-271 T cell lines very efficiently and specifically. Example 10. In vivo Inhibition of Priming of Lymph Node Cells SJL mice were injected intradermally in the hind footpads with lOμg of pl99-212 (lOOμl) in CFA, concomitant with intravenous inoculation of a competitor peptide in PBS (500μl) . The competitor peptides were 207Ala, 207Ala-262Lys and 262Lys- 207Ala. Lymph node cells obtained from the mice were incubated in the presence of various concentrations of pl99-212 for 96h. Thereafter 3 H-thymidine was added and I6h later cells were harvested and radioactivity was counted. Results, expressed as percent inhibition of the pl99-2l2 specific proliferative response, are shown in Figure 12. BALB/c mice were injected intradermally in the hind footpads with lOμg of p259-271 in CFA (lOOμl) concomitant with an i.v. inoculation of the analogs 262Lys, 207Ala-262Lys or 262Lys-207Ala, (200μg) in 500μl of PBS. Lymph node cells obtained from the mice were incubated in the presence of various concentrations of p259-271 for 96 h. Thereafter 3 H- thymidine was added and 16 h later cells were harvested and radioactivity was counted. Results, expressed as percent of inhibition of the p259-27l specific proliferative response, are shown in Figure 13. Example 11. Inhibition of Lymph Node Cell Proliferation by Oral Feeding with the Dual Analog In order to assess the efficacy of the dual analog 262Lys-207Ala in inhibiting T cell proliferative responses to the myasthenogenic T cell epitopes, namely pl95-212 and p259- 271, the following experiments were performed: 1) SJL mice were injected intradermally in the hind footpads with lOμg of pl95-212 in CFA (lOOμl) concomitant with an either i.v. or i.p. inoculation of the dual analog 262Lys- 207Ala (200μg) in 500μl of PBS. Two additional groups were fed with either 205μg/mouse or 500μg/mouse of the dual analog in 300μl of PBS. Ten days later the mice were sacrificed and their lymph node cells were incubated in the presence of different concentrations of pl95-212 for 96 hours. Thereafter 3 H-thymidine was addεd and 16 hours later cells were harvested and radioactivity was counted. Figure 14 represents the stimulation indices obtained in the proliferation assay of the lymph node cells in the different groups. As can be seen, the efficient proliferation obtained in the group that was only immunized with pl95-2l2 was completely inhibited in the groups that were either injected with 200μg of the dual analog in PBS i.v. or i.p. or fed with 500μg of the analog. A dramatic inhibition was observed in the group of mice that was fed with 250μl as well. Figure 15 shows the percentage of inhibition obtained in the latter experiment. As can be seen, oral administration of 500μg of the dual analog completely inhibited the proliferation of lymph node cells to the myasthenogenic T-cell epitope, pl95-212. 2) Figure 16 demonstrates the results of an experiment in which BALB/c mice were immunized with the second myasthenogenic peptide, namely p259-271 in CFA (20μg/mouse) and were either injected with the. dual analog 262Lys-207Ala i.v. (200μg/mouse in PBS) or fed with 500μg/mouse in PBS. Results are expressed in the figure as percentages of inhibition of the proliferative responses in the presence of different concentrations of the myasthenogenic epitope, p259-27l. As can be seen, the per os route of administration of the dual analog is at least as effective in inhibiting the proliferative response as the i.v. injection. In order to prevent or cure the induced experimental MG, peptide analogs may be administered a) before inoculation of the peptide specific T cells; b) concomitant with inoculation of the peptide specific T cells; c) after inoculation with the peptide specific T cells but before the appearance of autoimmune responses; or d) after the appearance of experimental MG. Reduction in the severity of the autoimmune response indicates suitability of treatment with the peptide. All references cited herein, including journal articles or abstracts, published or corresponding U.S. or foreign patent applications, issued U.S. or foreign patents, or any other references, are entirely incorporated by reference herein, including all data, tables, figures, and text presented in the cited references. Additionally, the entire contents of the references cited within the references cited herein are also entirely incorporated by reference. Reference to known method steps, conventional method steps, known methods or conventional methods is not in any way an admission that any aspect, description or embodiment of the present invention is disclosed, taught or suggested in the relevant art. The foregoing description of the specific embodiments will so fully reveal the general nature of the invention that others can, by applying knowledge within the skill of the art (including the contents of the references cited herein) , readily modify and/or adapt for various applications such specific embodiments, without undue experimentation, without departing from the general concept of the present invention. Therefore, such adaptations and modifications are intended to be within the meaning and range of equivalents of the disclosed embodiments, based on the teaching and guidance presented herein. It is to be understood that the phraseology or terminology herein is for the purpose of description and not of limitation, such that the terminology or phraseology of the present specification is to be interpreted by the skilled artisan in light of the teachings and guidance presented herein, in combination with the knowledge of one of ordinary skill in the art. SEQUENCE LISTING (1) GENERAL INFORMATION: (l) APPLICANT: YEDA RESEARCH AND DEVELOPMENT CO. LTD. (ll) TITLE OF INVENTION: SYNTHETIC PEPTIDES AND PHARMACEUTICAL COMPOSITIONS COMPRISING THEM (in) NUMBER OF SEQUENCES: 18 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Paulina Ben-Ami Yeda Research and Development Co. Ltd. (B) P.O. BOX: 95 (C) CITY: Rehovot (D) COUNTRY: ISRAEL (E) ZIP: 76100 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.30 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (vii) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: US 08/475,577 (B) FILING DATE: 07-JUN-1995 (vm) ATTORNEY/AGENT INFORMATION: (A) NAME: BEN-AMI, Paulina (B) REFERENCE/DOCKET NUMBER: 9237 PCT/2 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 08-9470617 (B) TELEFAX: 08-9470739 (2) INFORMATION FOR SEQ ID Nθ:l: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: l : Asp Thr Pro Tyr Leu Asp He Thr Tyr His Phe Val Met Gin Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: Val He Val Glu Leu He Pro Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE : peptide ( i) SEQUENCE DESCRIPTION: SEQ ID NO: 3 : Asp Thr Pro Tyr Leu Lys He Thr Tyr His Phe Val Met Gin Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO: : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: Asp Thr Pro Tyr Leu Asp He Thr Phe His Phe Val Met Gin Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: Asp Thr Pro Tyr Leu Asp He Thr Tyr Gly Phe Val Met Gin Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: ASD Thr Pro Tyr Leu Asp He Thr Tyr His Phe Val Ala Gin Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: Asp Thr Pro Tyr Leu ASD He Thr Tyr His Phe Val Met Asp Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: Asp Thr Pro Tyr Leu Asp He Thr Tyr His Phe Val Met Asn Arg Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: Asp Thr Pro Tyr Leu Asp He Thr Tyr His Phe Val Met Gin Lys Leu 1 5 10 15 Pro Leu (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: Val He Val Asp Leu He Pro Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: Val He Val Lys Leu He Pro Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: Val He Val Ser Leu He Pro Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO-.13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: Val He Val Glu Leu He Ala Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: Val He Val Glu Leu He Leu Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:IS: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: Val He Val Glu Leu He Phe Ser Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: Val He Val Glu Leu He Pro Asp Thr Ser Ser Ala Val 1 5 10 (2) INFORMATION FOR SEQ ID NO:17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 31 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: Asp Thr Pro Tyr Leu Asp He Thr Tyr His Phe Val Ala Gin Arg Leu 1 5 10 15 Pro Leu Val He Val Lys Leu He Pro Ser Thr Ser Ser Ala Val 20 25 30 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 31 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: Val He Val Lys Leu He Pro Ser Thr Ser Ser Ala Val Asp Thr Pro 1 5 10 15 Tyr Leu Asp He Thr Tyr His Phe Val Ala Gin Arg Leu Pro Leu 20 25 30

Claims: CLAIMS 1. A response-inhibiting peptide of at least fourteen amino acid residues capable of inhibiting the proliferative response of T lymphocytes from a myasthenia gravis patient to a myasthenogenic peptide corresponding to a sequence of the human acetylcholine receptor α-subunit selected from the group consisting of peptide pl95-212 having the formula of SEQ ID NO:l: 195 200 205 Asp-Thr-Pro-Tyr-Leu-Asp-Ile-Thr-Tyr-His-Phe- 210 Val-Met-Gln-Arg-Leu-Pro-Leu and the peptide p259-271 having the formula of SEQ ID NO:2: 260 265 270 Val-Ile-Val-Glu-Leu-Ile-Pro-Ser-Thr-Ser-Ser-Ala-Val said response-inhibiting peptide comprising two linked amino acid sequences wherein the first amino acid sequence includes at least amino acid residues 200-208 of SEQ ID N0:1 and the second amino acid sequence includes at least amino acid residues 262-266 of SEQ ID NO:2, but said sequences of the response-inhibiting peptide differing from SEQ ID NO:l and SEQ ID NO:2 by one to three amino acid substitutions in each of SEQ ID NO:l and NO:2, said substitutions being selected so as to have the function of inhibiting the proliferative response of T lymphocytes from a myasthenia gravis patient to the myasthenogenic peptides of SEQ ID NO:l and SEQ ID NO:2, said amino acid substitutions being selected from those which cumulatively do not substantially change the volume, hydrophobic-hydrophilic pattern and charge of the corresponding portions of the unsubstituted myasthenogenic peptides. 2. A response-inhibiting peptide in accordance with claim 1 having only a single said amino acid substitution in each of SEQ ID NO:l and SEQ ID N0:2. 3. A response-inhibiting peptide in accordance with claim 2, wherein said single amino acid substitutions are at least in one of the amino acids 200, 203, 204, 207 or 208 of SEQ ID N0:1, and at least in one of the amino acids 262, 265 or 266 of SEQ ID NO:2. 4. A response-inhibiting peptide in accordance with any one of claims 1 to 3, wherein said substitutions are selected from the group consisting of: 200, Asp-->Lys; 203, Tyr-->Phe; 204, His-->Gly; 207, Met-->NLeu; 207, Met-->Ala ; 208, Gln-->Asp; 208, Gln-->Asn; 262, Glu-->Asp; 262, Glu-->Lys; 262, Glu-->Ser; 262, Glu-->Ala; 265, Pro-->Leu; 265, Pro-->Phe; 266, Ser-->Lys; and 266, Ser-->Asp. 5. A response-inhibiting peptide in accordance with claim 1, wherein at least one of said substitutions is the substitution of one hydrophobic amino acid residue with another hydrophobic amino acid residue. 6. A response-inhibiting peptide in accordance with claim 1, wherein at least one of said substitutions is the substitution of one hydrophilic non-charged residue with a charged or non-charged hydrophilic residue. 7. A response-inhibiting peptide in accordance with claim 1, wherein at least one of said substitutions is the substitution of one charged hydrophilic amino acid residue with a charged hydrophilic amino acid residue of the same or opposite charge or a non-charged hydrophilic amino acid residue . 8. A response-inhibiting peptide in accordance with claim 1, wherein at least one of said substitutions is the substitution of one hydrophilic amino acid residue with a hydrophobic amino acid residue. 9. A response-inhibiting peptide in accordance with claim 1, wherein at least one of said substitutions is the substitution of one hydrophobic amino acid residue with a hydrophilic amino acid residue. 10. A response-inhibiting peptide in accordance with claim 1, wherein the two amino acid sequences are covalently linked to one another. 11. A response-inhibiting peptide in accordance with claim 10 comprising 25-31 amino acid residues. 12. A response-inhibiting peptide in accordance with claim 10 comprising 31 amino acid residues. 13. A response-inhibiting peptide comprising 31 amino acid residues of SEQ ID NO: 17 in which the N-terminal sequence corresponds to SEQ ID N0:1 but differs therefrom by a single substitution of 207Met to 207Ala and the C-terminal sequence corresponds to SEQ ID NO:2 but differs therefrom by a single substitution of 262Glu to 262Lys. 14. A response-inhibiting peptide comprising 31 amino acid residues of SEQ ID NO:18 in which the N-terminal sequence corresponds to SEQ ID NO:2 but differs therefrom by a single substitution of 262Glu to 262Lys and the C-terminal sequence corresponds to SEQ ID N0:1 but differs therefrom by a single substitution of 207Met to 207Ala. 15. A response-inhibiting peptide in accordance with claim 1, wherein the two amino acid sequences are linked together by a chemical linker or link sequences. 16. A response-inhibiting peptide in accordance with claim 15, wherein said first and second sequences are linked together by a shore stretch of alanine residues. 17. A response-inhibiting peptide in accordance with claim 15, wherein said first and second sequences are linked together by a site for proteolysis by cathepsin. 18. A response-inhibiting peptide according to claim 1, conjugated to a macromolecular carrier. 19. A response-inhibiting peptide according to claim 18, conjugated to a protein carrier. 20. A response-inhibiting peptide according to claim 19, wherein the protein carrier is tetanus toxoid. 21. A response-inhibiting peptide according to claim 20, wherein the macromolecular carrier is a copolymer of amino acids. 22. A pharmaceutical composition comprising a peptide of SEQ ID NO:17 and a pharmaceutically acceptable excipient. 23. A pharmaceutical composition comprising a peptide of SEQ ID NO:18 and a pharmaceutically acceptable excipient. 2 . A pharmaceutical composition for the treatment of myasthenia gravis comprising an effective amount of a response-inhibiting peptide in accordance with claim 1 and a pharmaceutically acceptable excipient. 25. A pharmaceutical composition according to any one of claims 22 to 24 adapted for oral administration. 26. Use of a response-inhibiting peptide in accordance with claim 1 for the manufacture of a pharmaceutical composition for the treatment of myasthenia gravis. 27. A method for the treatment of myasthenia gravis comprising administering to a myasthenia gravis patient an effective amount of a response-inhibiting peptide in accordance with claim 1.

Inventors: MOZES EDNA, SELA MICHAEL, MOZES, EDNA, SELA, MICHAEL
Applicants: YEDA RES & DEV, YEDA RESEARCH AND DEVELOPMENT CO. LTD.
Application year: 1996
Main classification: C07K14
IPC codes: A61K38, A61P43, C07K14