HETEROCYCLIC COMPOUNDS, THEIR PREPARATION AND USE

Abstract: The present invention relates to a method of treating a disease in the central nervous system via the metabotropic glutamate receptor system, the use of known as well as novel pyridino(2,3-b) indoles (Ib) for the preparation of a medicament for treating said diseases, novel therapeutic active pyridino(2,3-b) indoles, a method for preparing the same and pharmaceutical compositions comprising these compounds.

Description: Heterocyclic compounds , their preparation and use . The present invention relates to a method of treating a disease in the central nervous system via the metabotropic glutamate receptor system, the use of known as well as new pyridino[2,3-b]indoles for the preparation of a me- dicament for treating said diseases, novel therapeutic active pyridino[2,3- b]indoles; a method for preparing the same and pharmaceutical composi¬ tions comprising these compounds. Recent molecular biological studies have clearly established the existence of two major types of glutamate receptors in the central nervous system namely the ionotropic and the metabotropic glutamate receptors. The latter is char¬ acterised by being G-protein-linked to changes in second messenger forma¬ tion and modulation of ion channel function, (Meldrum, B. (1991) Epilepsy Res. 10, 55-61 , Chapman, A. (1991) in Excitatory Amino Acids p. 265-286, Blackwell scientific publ. ltd., Oxford). At present 8 different subtypes of the metabotropic glutamate receptors are described (MGIUR T to MGIuR 8 ) and in addition some spliced variants of the subtypes are reported. The Metabotropic glutamate receptor subtypes MGIUR T and MGIuR 5 are coupled to phosphoinositide hydrolysis (Johnson, G. and Bigge, CF. (1991) Annu. Rep. Med. Chem. 26, 11-22, Hansen, J.J. and Krogsgaard Larsen, P. Med. Res. Rev. 10,55-94, Thomsen, C. and Suzdak, P. (1993) Eur. J. Pharmacol. 245 ,299), while the others are coupled to cyclic AMP formation (Schoepp, D.D., Johnson, B.G. and Monn, J.A. (1992) J. Neurochem. 58, 1184-1186, Cartmell et al. (1992) J. Neurochem. 58, 1964-1966, Manzoni, O. et al. (1992) Eur. J. Pharmacol. 225, 357-358). Compounds such as L-glutamate, quisqualate and ibotenate are known to act as non-selective agonists on the metabotropic glutamate receptors, while selective ionotropic glutamate receptor agonists such as NMDA, AMPA and kainate have little effect on these receptors. Recently a few compounds without activity at the ionotropic glutamate receptors but with activity at the metabotropic receptors have been identified. These comprise trans-ACPD (trans 1S,3R-1-aminocyclopentane-1 ,3-dicar- boxylic acid), the partial agonist L-AP3 (L-2-amino-3-phosphonopropionic acid) (Palmer, E., Monaghan, D.T. and Cotman, CW. (1989) Eur. J. Phar¬ macol. 166, 585-587, Desai, M.A. and Conn, P.J. (1990) Neurosci. Lett. 109, 157-162, Schoepp, D.D. et al. (1991), J. Neurochem. 56, 1789-1796, Schoepp D.D. and Johnson B.G. (1989), J. Neurochem. 53,1865-1613), L-AP4 (L-2-amino-4-phosphonobutyrate) which is an agonist at the MGIuR 4 receptor (Thomsen C et al. (1992), Eur. J. Pharmacol. 227, 361-362) and some of the isomers of CCG (2-(carboxycyclopropyl)glycines) especially L-CCG-I and L-CCG-II (Hayashi, Y. et al. (1992), Br. J. Pharmacol. 107, 539- 543). Very few selective antagonists at the metabotropic glutamate receptors have been reported, however some phenylglycine derivatives S-4CPG (S-4-carboxyphenyl glycine), S-4C3HPG (S-4-carboxy-3-hydroxyphenyl glycine) and S-MCPG ( S-alpha methyl-4-carboxyphenyl glycine) have been reported to antagonise trans ACPD stimulated phosphoinositide hydrolysis and thus possibly acting as antagonists at the metabotropic glutamate receptors at the subtypes MGluR ! and MGIuR 5 (Thomsen, C. and Suzdak, P, (1993) Eur. J. Pharmacol. 245, 299). Literature evidence suggests that compounds selective for the metabotropic glutamate receptors either as agonists or antagonists are useful in the treatment of different neurological diseases. The use of compounds active at the metabotropic glutamate receptors for the treatment of epilepsy is corroborated by investigations of the influence of trans-ACPD in the formation of convulsions (Sacaan and Schoepp, (1992), Neurosci. lett. 139, 77) and that phosphoinositide hydrolysis mediated via MGluR is increased after kindling experiments in rats (Akiyama et al. (1992),Brain Res. 569, 71). Trans-ACPD has been shown to increase release of dopamine in the rat brain which indicates that compounds acting on the metabotropic glutamate receptors might be usable for the treatment of Parkinson's disease and Huntington's Chorea (Sacaan et al. (1992), J. Neurochem. 59, 245). The use of compounds active at the metabotropic glutamate receptors for treatment of neurological diseases such as senile dementia has been indicated by the findings of Zheng and Gallagher ((1992), Neuron 9, 163) and Bashir et al. ((1993), Nature 363, 347) who demonstrated that activation of metabotropic glutamate receptors are necessary for the induction of long term potentiation (LTP) in nerve cells (septal nucleus, hippocampus) and the finding that long term depression is induced after activation of metabotropic glutamate receptors in cerebellar granule cells (Linden et al. (1991), Neuron 7,81). Investigations also show that in the treatment of deficiencies of mental and motoric performance seen after conditions of brain ischemia the metabotro¬ pic glutamate receptor active compounds may prove usable. Trans-ACPD has been shown to be a neuroprotective agent in an MCAO model in mice (Chiamulera et al. (1992), Eur. J. Pharmacol. 215, 353), and it has been shown to inhibit NMDA induced neurotoxicity in nerve cell cultures (Koh et al., (1991), Proc. Natl. Acad. Sci. USA 88, 9431). Also in the treatment of pain the metabotropic glutamate receptor active compounds seem of interest, proved by the fact that antagonists at the metabotropic glutamate receptors antagonises sensory synaptic response to noxious stimuli of thalamic neurons (Eaton, S.A. et al. (1993), Eur. J. Neuro¬ sci. 5, 186). The above findings support that compounds acting on the metabotropic glutamate receptors are useful for the treatment of epilepsy, neurological diseases such as senile dementia, Parkinson's disease, Huntington's Cho¬ rea, pain and deficiencies of mental and motoric performance seen after conditions of brain ischemia. We have now discovered that a series of pyridino[2,3-b]indoles, some of which compounds are known from GB 1 268 772 (Glaxo Laboratories Ltd.) to possess antiviral activity, are potent antagonists at the metabotropic gluta- mate receptors. Thus the present invention is concerned with a method of treating a disease in the central nervous system via the metabotropic glutamate receptor system comprising administering to a subject in need thereof an effective amount of a compound of formula lb wherein R 1b is H; C^-alkyl optionally substituted with halogen; C 2.6 -alkenyl; C 2.6 - alkynyl; C 3 ^-cycloalkyl; C^-alkyl substituted with Ca^-cycloalkyl; carboxy; COR 9 ; -COOR 9b ; C 1-6 -alkyl substituted with dimethylamino; -R 9b -O-R 10b ; -R 9b -O-R 10b -O-R 11 ; phenylsulfonyl; benzoyl; benzyl or phenyl each of which aromatic group is optionally substituted with C^-alkyl, C^-alkoxy, halogen, carboxy or nitro; wherein R 9b , R 10b , and R 11b are inde¬ pendently C^e-alkyl; R 2b is amino optionally substituted with one or two C^-alkyl which alkyl group(s) is/are optionally substituted with hydroxy, morpholino, amino unsubstituted or mono or disubstituted with C^-alkyl, phenyl, phenylsulfonyl or benzyl; piperidino; morpholino; or piperazino optionally N-substituted with C^-alky!; R 3b is H; carboxy; cyano; nitro; C^-alkyl optionally substituted with hydroxy; - R 9b -O-R 10b ; -COOR 9b ; wherein R 9b and R 10b are as defined above; morpholi- nocarbonyl; thiomorpholinocarbonyl; piperazinylcarbonyl optionally substi- tuted with C^-alkyl; tetrazolyl; oxadiazolyl or thiadiazolyl optionally substi¬ tuted with C^-alkyl or C 3 . 6 -cycloalkyl; morpholinomethyl; amino unsubstituted or mono or disubstituted with C^-alkyl; methylamino unsubstituted or N- mono or disubstituted with C^-alkyl; sulfamoyl unsubstituted or mono or disubstituted with C^-alkyl; or carbamoyl unsubstituted or mono or disubsti- tuted with C^-alkyl which alkyl group(s) is/are optionally substituted with dimethylaminomethyl, halogen, phenyl or benzyl; R 4b is H; or C^-alkyl; R Sb , R 6b , R 7 , and R 8b are independently H; nitro; amino; halogen; trifluoromethyl; trifluoroacetyl; sulfo; carboxy; carbamoyl; sulfamoyl; -COR 9b ; -COOR 9 ; wherein R 9b is as defined above; C^-alkoxy; or C^-alkyl optionally substituted with halogen; or a salt thereof with a pharmaceutically acceptable acid or base. These salts include pharmaceutically acceptable acid addition salts, phar¬ maceutically acceptable metal salts or optionally alkylated ammonium salts, such as hydrochloric, hydrobromic, hydroiodic, phosphoric, sulfuric, trifluo¬ roacetic, trichloroacetic, oxalic, maleic, pyruvic, malonic, succinic, citric, tartaric, fumaric, mandelic, benzoic, cinnamic, methanesulfonic, ethane sulfonic, picric and the like, and include acids related to the pharmaceutically acceptable salts Iisted in Joumal of Pharmaceutical Science, 66, 2 (1977) and incorporated herein by reference, or lithium, sodium, potassium, magne¬ sium and the like. The term "C^-alkyl" as used herein, alone or in combination, refers to a . straight or branched, saturated hydrocarbon chain having 1 to 6 carbon atoms such as e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, sec.butyl, isobutyl, tert.butyl, n-pentyl, 2-methylbutyl, 3-methylbutyl, n-hexyl, 4-methyl- pentyl, neopentyl, n-hexyl and 2,2-dimethylpropyl. The term "C- 1-6 -alkoxy" as used herein, alone or in combination, refers to a monovalent substituent comprising a lower alkyl group linked through an ether oxygen having its free valence bond from the ether oxygen and having 1 to 6 carbon atoms e.g. methoxy, ethoxy, propoxy, butoxy, pentoxy. The term "C 2 . 6 -alkenyl" as used herein refers to an unsaturated hydrocarbon chain having 2-6 carbon atoms and one double bond such as e.g. vinyl, 1- propenyl, allyl, isopropenyl, n-butenyl, n-pentenyl and n-hexenyl. The term "C 3 . 6 -cycloalkyl" as used herein refers to a radical of a saturated cyclic hydrocarbon with the indicated number of carbons such as cyclopro¬ pyl, cyclobutyl, cyclopentyl, or cyclohexyl. The term "C^-alkynyl" as used herein refers to unsaturated hydrocarbons which contain triple bonds, such as e.g. -C≡CH, -C≡CCH 3 , -CH 2 C≡CH, -CH 2 CH 2 C * =CH, -CH(CH 3 )C≡H, and the like. The term "halogen" means fluorine, chlorine, bromine and iodine. It is to be understood that the invention extends to the use of each of the stereoisomeric forms of the compounds of formula lb as well as the race- mates. In the method of the present invention, R 1b is preferably benzyl optionally substituted with C^-alky!, C^-alkoxy, halogen, carboxy or nitro, more preferably benzyl optionally substituted with halogen; R 2b is preferably piperidino; morpholino; or piperazino optionally N-substituted with C^-alkyl; more preferably morpholino; and R 3b is preferably carboxy. The present invention furthermore relates to the use of compounds of formula lb for the preparation of a medicament for treatment of a disease in the central nervous system via the metabotropic glutamate receptor system. In addition, the present invention is concerned with novel compounds of formula lb wherein R 1b is benzyl optionally substituted with C^-alkyl, C,. 6 -alkoxy, halogen, carboxy or nitro, preferably benzyl optionally substituted with halogen; R 2b is piperidino; morpholino; or piperazino optionally N-substituted with C h ¬ alky!; preferably morpholino; R 3b is H; carboxy; cyano; nitro; C^-alkyl optionally substituted with hydroxy; - R 9b -O-R 10b ; -COOR 9b ; wherein R 9b and R 10b are as defined above; morpholi- nocarbonyl; thiomorpholinocarbonyl; piperazinylcarbonyl optionally substi¬ tuted with C 1-6 -alkyl; tetrazolyl; oxadiazolyl or thiadiazolyl optionally substi¬ tuted with C 1-6 -alkyl or C 3 . 6 -cycloalkyl; morpholinomethyl; amino unsubstituted or mono or disubstituted with C^-alkyl; methylamino unsubstituted or N- mono or disubstituted with C^-alkyl; sulfamoyl unsubstituted or mono or disubstituted with C^-alkyl; or carbamoyl unsubstituted or mono or disubsti¬ tuted with C 1-6 -alkyl which alkyl group(s) is/are optionally substituted with dimethylaminomethyl, halogen, phenyl or benzyl; preferably carboxy. R 4 is H; or C^-alkyl; R 5 , R 6 , R 7b , and R 8b are independently H; nitro; amino; halogen; trifluoromethyl; trifluoroacetyl; sulfo; carboxy; carbamoyl; sulfamoyl; -COR 9b ; -COOR 9 ; wherein R 9b is as defined above; C^-alkoxy; or C^-alkyi optionally substituted with halogen; or a salt thereof with a pharmaceutically acceptable acid or base. Specific examples of compounds of the invention include the following: Ethyl 9-benzyl-2-piperidinopyridino[2,3-b]indole-3-carboxylate, Ethyl 9-benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylate, 9-Benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylic acid, 9-Benzyl-2-morpholinopyridino[2,3-b]indole, 9-Benzyl-2-piperidinopyridino[2,3-b]indole. The compounds of formula lb may e.g. be prepared in accordance with the teaching of GB 1 268 772. However, the invention also relates to an advan¬ tageous method of preparing the above mentioned compounds. This method comprises a) reacting a compound of formula lib, prepared by well known methods, wherein R 1b , R 5b , R 6b , R 7b and R 8 have the meanings defined above, with a N,N-dimethyl amide, preferably dime- thylformamide or dimethylacetamide, and POX 3 , wherein X is chlorine or bromine, using Vilsmeyer-Hack conditions, to form a compound of formula 1Mb, wherein X is chlorine or bromine, and R 1 , R 4 , R 5b , R 6b , R 7b , and R 8 have the meanings defined above; and subsequently b) reacting a compound of formula lllb, wherein X is chlorine or bromine, and R 1b , R 4b , R 5 , R 6b , R 7b , and R 8b have the meanings defined above, with a compound N≡C-CH 2 -Z wherein Z is cyano, nitro, COOR 9b wherein R 9b have the meanings defined above, sulfamoyl unsubstituted or mono or disubsti- tuted with C^-alkyl, or carbamoyl unsubstituted or mono or disubstituted with C 1-6 -alkyl; together with an amine as defined for R 2 , preferably morpholine or piperidine, either in the presence or absence of an added strong base, such as sodium hydride, to form a compound of formula IVb, wherein Z , R 1 , R 2 , R , R 5b , R 6b , R 7b , and R 8 have the meanings defined above, or c) reacting a compound of formula IVb, wherein Z b , R 1b , R 2b , R b , R 5b , R 6 , R 7b , and R 8b have the meanings defined above, by means of well known chemical reactions transforming the Z b group to hydrogen or to other func¬ tional groups such as acids, esters, amides, amines, or reaction products thereof as described for R 3b , to form a compound of formula lb, wherein R 1b , R 2b , R 3 , R 4b , R 5b , R 6b , R 7b , and R 8b have the meanings defined above, or d) reacting a compound of formula formula lb, wherein R 1b , R 2b , R 3 , and R 4b have the meanings defined above, and at least one of R 5b , R 6b , R 7b , or R 8b is H with well known reactive substrates leading to aromatic substitution using the reaction conditions known in the art, to form a compound of formula lb, wherein R 5 , R 6b , R 7 , or R 8 have the meanings defined above provided that at least one of R 5b , R 6b , R 7b , or R 8b is not H, or e) a compound of formula lb or formula Mb or formula IVb with different R 5 , R 6b , R 7 , or R 8b could be prepared by using conventional organic chemistry on functional groups already introduced as R 5b , R 6b , R 7 , or R 8 groups. The pharmacological properties of the compounds of the invention can be illustrated by determining their effects in different conventional radioligand binding assays or in functional in vitro assays. The compounds of the invention were studied in an in vitro assay for meas¬ uring inhibition of Pl-hydrolysis in BHK 570 cells expressing mGluR^ receptors. Principle The metabotropic glutamate receptor (mGluR) is selectively activated by trans-aminocyclopentane dicarboxylic acid and is coupled to the hydrolysis of inositol phosphates via a GTP-binding protein. At the molecular level, cDNAs encoding six subtypes of the mGluR family have been isolated. The first subtype isolated (Houamed et al., 1991 , Science 252, 1318), termed the mGIuRlα, has been shown to be coupled to Pl-hydrolysis when expressed in baby hamster kidney cells (BHK) (Thomsen et al., Brain Res. (in press)). In these cells no stimulation by 1 mM quisqualate or glutamate was observed with control BHK cells whereas a 6-8 fold increase over basal Pl-hydrolysis was seen with BHK cells expressing mGIuRlα. Cell culture BHK570 cells expressing mGIuRlα are cultured in DMEM (4.5 g/l glucose, 2mM glutamin); 5% foetal calf serum; 0.10 mg/ml neomycin; 0.5 mg/ml G418; 1 μM methotrexate; 50 μg/ml gentamycin. Cells are subcultured every 5 days using 0.05% trypsin/EDTA in PBS. Inositol phosphate formation The protocol for Pl-hydrolysis was measured using a modification of a method previously described (Berridge et al., 1982, Biochem. J. 206,587). Cells were plated in 16 mm wells (24 well multidish, Costar) with 1 confluent 100 mm dish per multidish. Replace the medium 24 h before the experiment with 500 μl fresh growth medium containing 4μCi/ml myo-[2- 3 H]inositol (specific activity 18 Ci/mmol, Amersham). The cells were washed twice with Krebs-Henseleit buffer (Sigma cat. # 3753: glucose 2.0 g/l, MgSO 4 0.141 g/l, KHPO 4 0.16 g/l, KCl 0.35 g/l, NaCI 6.90 g/l and NaHCO 3 2.1 g/l) supple¬ mented with 10 mM LiCl and 2.5 mM CaCI 2 . The buffer was equilibrated with 5% CO 2 , 95% air to pH 7.5 at 37°C Following 5 min of preincubation in the above buffer, buffer or test compounds were added and cells were incubated for 30 min at 37°C In antagonist studies, add test compounds 5 min prior to agonist stimulation. Pl-formation was stopped by placing the cells on ice and quickly aspirating the media. The wells were washed once with ice-cold Krebs-Henseleit buffer and subsequently 1 ml ice-cold 10% perchloric acid was added to each well. Place the cells on ice for 20 min. In Nunc minisorp test tubes (75 x 12 mm, cat. # 443990): add 250 μl of 10 mM EDTA, pH 7.0 + 5% Universal Indicator (Merck). Transfer the PCA extract to each tube containing the pH-indicator. Neutralize the samples with 1.5 M KOH + 60 mM HEPES to pH 7.5 (~ 1100-1200 μl). Centrifugate (6.000 rpm, 5 min, 0°C). They can be stored frozen at this point. Fractions of inositolphosphates were separated using ion-exchange columns (Amersham, RPN 1908) according to the method provided by Amersham. Separation of inositol phosphates on ion-exchange columns Prepare columns with 5 ml 1 M KHCO 3 and wash with 15 ml dist. water. Adjust vacuum so that the flow-rate does not exceed 5 ml/min. Add 4 ml dist. water and subsequently 1 ml [ 3 H]lnsP sample. Wash with 5 ml dist. water. IP1 to IP4 fractions may be collected with 5 ml 0.05; 0.10; 0.17 and 0.25 M KHCO 3 , respectively. Usually IP1 and IP2 fractions are collected simultaneously. Scintillation liquid: use 12-15 ml Ultima Gold (Packard). Testprocedure Testcompounds are dissolved in DMSO, DMSO and Pluronic F-127 or ethanol and diluted in assay buffer. Glutamate (10 μM and 1000 μM) and buffer alone are included as a control. Results The stimulation by 10 μM shall represent a submaximal stimulation. The response by 10 μM glutamate should exceed 3-fold the basal level and should be below maximal stimulation (glutamate at 1 mM). The results are calculated relative to the stimulation by 10 μM glutamate and a dose re¬ sponse curve is generated. An example of a test result obtained by testing a compound of the present invention in the above mentioned assay appear from the following Table 1. Table 1 Compound No. IC 50 (μM) 3 10 The compounds according to the invention are effective over a wide dosage range. For example, in the treatment of adult humans, dosages from about 0.05 to about 100 mg, preferably from about 0.1 to about 100 mg, per day may be used. A most preferable dosage is about 10 mg to about 70 mg per day. In choosing a regimen for patients suffering from a disease in the central nervous system related to the metabotropic glutamate receptor system it may frequently be necessary to begin with a dosage of from about 30 to about 70 mg per day and when the condition is under control to reduce the dosage as low as from about 1 to about 10 mg per day. The exact dosage will depend upon the mode of administration, form in which admin¬ istered, the subject to be treated and the body weight of the subject to be treated, and the preference and experience of the physician or veterinarian in charge. The route of administration may be any route, which effectively transports the active compound to the appropriate or desired site of action, such as oral or parenteral e.g. rectal, transdermal, subcutaneous, intravenous, intramus¬ cular or intranasal, the oral route being preferred. Typical compositions include a compound of formula lb or a pharmaceutically acceptable acid addition salt thereof, associated with a pharmaceutically acceptable carrier. In making the compositions, conventional techniques for the preparation of pharmaceutical compositions may be used. For example, the active compound will usually be mixed with a carrier, or diluted by a carrier, or enclosed within a carrier which may be in the form of a ampoule, capsule, sachet, paper, or other container. When the carrier serves as a diluent, it may be solid, semi-solid, or liquid material which acts as a vehicle, excipient, or medium for the active compound. The active compound can be adsorbed on a granular solid container for example in a sachet. Some examples of suitable carriers are water, salt solutions, alcohols, polyethylene glycols, polyhydroxyethoxylated castor oil, gelatine, lactose, amylose, magnesium stearate, talc, silicic acid, fatty acid monoglycerides and diglyc¬ erides, pentaerythritol fatty acid esters, hydroxymethylcellulose and poly¬ vinylpyrrolidone. The pharmaceutical preparations can be sterilized and mixed, if desired, with auxiliary agents, emulsifiers, salt for influencing osmotic pressure, buffers and/or coloring substances and the like, which do not deleteriously react with the active compounds. For parenteral application, particularly suitable are injectable solutions or suspensions, preferably aqueous solutions with the active compound dis- solved in polyhydroxylated castor oil. Tablets, dragees, or capsules having talc and/or a carbohydrate carrier or binder or the like are particularly suitable for oral application. Preferable carriers for tablets, dragees, or capsules include lactose, corn starch, and/or potato starch. A syrup or elixir can be used in cases where a sweetened vehicle can be employed. Generally, the compounds are dispensed in unit form comprising from about 1 to about 100 mg in a pharmaceutically acceptable carrier per unit dosage. A typical tablet, appropriate for use in this method, may be prepared by conventional tabletting techniques and contains: Active compound 5.0 mg Lactosum 67.8 mg Ph. Eur. Avicel® 31.4 mg Amberlite® 1.0 mg Magnesii stearas 0.25 mg Ph. Eur. The invention will now be described in further detail with reference to the following examples. EXAMPLE 1b Ethyl 9-benzyl-2-piperidinopyridino[2,3-b]indole-3-carboxylate (1 b) To a slurry of 1.35 g of 1-benzyl-2-chloroindoIe-3-carbaldehyde in 30 ml abs. EtOH was added 1 ml of ethyl cyanoacetate and 3.5 ml of piperidine, and the mixture was stirred at room temperature for 2.5 days. 30 ml of ice/water was added, and the precipitate was isolated by filtration and dried to give (1b). Yield 1.6 g of (1 b), m.p. 155-156 °C EXAMPLE 2b Ethyl 9-benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylate (2b) To a slurry of 1.35 g of 1-benzyl-2-chloroindole-3-carbaldehyde in 30 ml abs. EtOH was added 1.5 ml of ethyl cyanoacetate and 3.5 ml of morpholine, and the mixture was stirred at room temperature for 2 days. The mixture was then heated to reflux for 5 hours, cooled to room temperature and filtered to give (2b). Yield 1.6 g of (2b), m.p. 151-152°C EXAMPLE 3b 9-Benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylic acid (3b) 1.3 g of (2b) was covered with a solution of 200 mg of KOH in 2 ml of water, and stirred at room temperature for 1 hour and at 60 °C for 2 hours. After cooling the solvent was evaporated and the product precipitated with water and acetic acid to give 1.1 g of a mixture of 2 compounds. These two prod¬ ucts were separated by column chromatography, on Silica 60 using metha¬ nol and methylenedichloride 1 + 9 as the eluent, to give (3b). Yield 480 mg of (3b). An analytical sample was recrystallized from EtOH, m.p. 282-287°C

Claims: CLAIMS 1. A method of treating a disease in the central nervous system via the metabotropic glutamate receptor system comprising administering to a subject in need thereof an effective amount of a compound of formula lb wherein R 1b is H; C^e-alkyl optionally substituted with halogen; C 2 . 6 -alkenyl; C 2 . 6 - alkynyl; C 3 . 6 -cycloalkyl; C^-alkyl substituted with C 3 . 6 -cycloalkyl; carboxy; - COR 9 ; -COOR 9b ; C 1-6 -alkyl substituted with dimethylamino; -R 9 -O-R 10b ; -R 9b -O-R 10b -O-R 11 ; phenylsulfonyl; benzoyl; benzyl or phenyl each of which aromatic group is optionally substituted with C^-alky!, C^-alkoxy, halogen, carboxy or nitro; wherein R 9b , R 10 , and R 11b are inde- pendently C^-alkyl; R b is amino optionally substituted with one or two C^-alkyl which alkyl group(s) is/are optionally substituted with hydroxy, morpholino, amino unsubstituted or mono or disubstituted with C^-alkyl, phenyl, phenylsulfonyl or benzyl; piperidino; morpholino; or piperazino optionally N-substituted with C 1-6 -alkyl; R 3 is H; carboxy; cyano; nitro; C^-alkyl optionally substituted with hydroxy; - R 9b _ 0 . Rl o . _ c0 0R 9 ; wherein R 9b and R 10b are as defined above; morpholi- nocarbonyl; thiomorpholinocarbonyl; piperazinylcarbonyl optionally substi¬ tuted with C^-alky!; tetrazolyl; oxadiazolyl or thiadiazolyl optionally substi- tuted with C^-alkyl or C 3 . 6 -cycloalkyl; morpholinomethyl; amino unsubstituted or mono or disubstituted with C^-alkyl; methylamino unsubstituted or N- mono or disubstituted with C^-alkyl; sulfamoyl unsubstituted or mono or disubstituted with C,. 6 -alkyl; or carbamoyl unsubstituted or mono or disubsti- tuted with C^-alkyl which alkyl group(s) is/are optionally substituted with dimethylaminomethyl, halogen, phenyl or benzyl; R 4b is H; or C^-alkyl; R 5b , R 6 , R 7b , and R 8b are independently H; nitro; amino; halogen; trifluoromethyl; trifluoroacetyl; sulfo; carboxy; carbamoyl; sulfamoyl; -COR 9 ; -COOR 9b ; wherein R 9b is as defined above; C^-alkoxy; or C^-alkyl optionally substituted with halogen; or a salt thereof with a pharmaceutically acceptable acid or base. 2. A method according to claim 1 wherein R 1b is benzyl optionally substituted with C^-alkyl, C^-alkoxy, halogen, carboxy or nitro, preferably benzyl optionally substituted with halogen. 3. A method according to claim 1 or 2 wherein R 2b is piperidino; morpholino; or piperazino optionally N-substituted with C^-alkyl; preferably morpholino. 4. A method according to any one of the preceding claims wherein R 3b is carboxy. 5. A method according to claim 1 wherein the compounds of formula lb is selected from the following: Ethyl 9-benzyl-2-piperidinopyridino[2,3-b]indole-3-carboxylate, Ethyl 9-benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylate, 9-Benzyl-2-morpholinopyrid ino[2 , 3-b]indole-3-carboxylic acid , 9-Benzyl-2-morpholinopyridino[2,3-b]indole, 9-Benzyl-2-piperidinopyridino[2,3-b]indole. 6. A method according to any one of the preceding claims wherein the disease is epilepsy, senile dementia, Parkinson's disease, Huntington's Chorea, pain or deficiencies of mental and motoric performance seen after conditions of brain ischaemia. 7. The use of a compound of formula lb wherein R 1b is H; C^-alkyl optionally substituted with halogen; C 2 . 6 -alkenyl; C 2.6 - alkynyl; C 3 . 6 -cycloalkyl; C^-alkyl substituted with C 3.6 -cycloalkyl; carboxy; - COR 9b ; -COOR 9b ; C^-alkyl substituted with dimethylamino; -R 9b -O-R 10b ; -R 9 -O-R 10b -O-R 1 ; phenylsulfonyl; benzoyl; benzyl or phenyl each of which aromatic group is optionally substituted with C^-alkyl, C^-alkoxy, halogen, carboxy or nitro; wherein R 9b , R 10 , and R 11b are inde¬ pendently C^-alkyl; R 2b is amino optionally substituted with one or two C^-alky! which alkyl group(s) is/are optionally substituted with hydroxy, morpholino, amino unsubstituted or mono or disubstituted with C 1-6 -alkyl, phenyl, phenylsulfonyl or benzyl; piperidino; morpholino; or piperazino optionally N-substituted with C^-alkyl; R 3 is H; carboxy; cyano; nitro; C^-alkyl optionally substituted with hydroxy; - R 9b _ 0 _ Rl o . _ C0 0R 9b ; wherein R 9b and R 10b are as defined above; morpholi- nocarbonyl; thiomorpholinocarbonyl; piperazinylcarbonyl optionally substi¬ tuted with C^-alkyl; tetrazolyl; oxadiazolyl or thiadiazolyl optionally substi- tuted with C^-alkyl or C^-cycloalkyl; morpholinomethyl; amino unsubstituted or mono or disubstituted with C^-alkyl; methylamino unsubstituted or N- mono or disubstituted with C^-alky!; sulfamoyl unsubstituted or mono or disubstituted with C^-alky!; or carbamoyl unsubstituted or mono or disubsti¬ tuted with C^-alkyl which alkyl group(s) is/are optionally substituted with dimethylaminomethyl, halogen, phenyl or benzyl; R 4 is H; or C^-alkyl; R 5b , R 6b , R 7b , and R 8 are independently H; nitro; amino; halogen; trifluoromethyl; trifluoroacetyl; sulfo; carboxy; carbamoyl; sulfamoyl; -COR 9b ; -COOR 9b ; wherein R 9 is as defined above; C^-alkoxy; or C^-alky! optionally substituted with halogen; or a salt thereof with a pharmaceutically acceptable acid or base; for the preparation of a medicament for treatment of a disease in the central nervous system via the metabotropic glutamate receptor system. 8. The use according to claim 7 wherein R 1b is benzyl optionally substituted with C,. 6 -alkyl, C^-alkoxy, halogen, carboxy or nitro, preferably benzyl optionally substituted with halogen. 9. The use according to claim 7 or 8 wherein R 2 is piperidino; morpholino; or piperazino optionally N-substituted with C^-alkyl; preferably morpholino. 10. The use according to any one of claims 7, 8 or 9 wherein R 3b is carboxy. 11. The use according to claim 7 wherein the compounds of formula lb is selected from the following: Ethyl 9-benzyl-2-piperidinopyridino[2,3-b]indole-3-carboxylate, Ethyl 9-benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylate, 9-Benzyl-2-morpholinopyridino[2,3-b]indole-3-carboxylic acid, 9-Benzyl-2-morpholinopyridino[2,3-b]indole, 9-Benzyl-2-piperidinopyridino[2,3-b]indole. 12. The use according to any one of claims 7, 8, 9, 10 or 11 wherein the disease is epilepsy, senile dementia, Parkinson's disease, Huntington's Chorea, pain or deficiencies of mental and motoric performance seen after conditions of brain ischaemia. 13. A compound of formula lb wherein R b is benzyl optionally substituted with C^-alkyl, C^-alkoxy, halogen, carboxy or nitro, preferably benzyl optionally substituted with halogen; R 2b is piperidino; morpholino; or piperazino optionally N-substituted with C^- alkyl; preferably morpholino; R 3 is H; carboxy; cyano; nitro; C^-alky! optionally substituted with hydroxy; R 9 »_O-R 10 ; -COOR 9 ; wherein R 9 and R 10b are as defined above; morpholi- nocarbonyl; thiomorpholinocarbonyl; piperazinylcarbonyl optionally substi¬ tuted with C^-alkyl; tetrazolyl; oxadiazolyl or thiadiazolyl optionally substi¬ tuted with C^-alkyl or C^-cycloalkyl; morpholinomethyl; amino unsubstituted or mono or disubstituted with C 1-6 -alkyl; methylamino unsubstituted or N- mono or disubstituted with C^-alkyl; sulfamoyl unsubstituted or mono or disubstituted with C^-alkyl; or carbamoyl unsubstituted or mono or disubsti¬ tuted with C^-alkyl which alkyl group(s) is/are optionally substituted with dimethylaminomethyl, halogen, phenyl or benzyl; preferably carboxy. R 4b is H; or C^-alkyl; R 5b , R 6 , R 7b , and R 8b are independently H; nitro; amino; halogen; trifluoromethyl; trifluoroacetyl; sulfo; carboxy; carbamoyl; sulfamoyl; -COR 9 ; -COOR 9 ; wherein R 9b is as defined above; C^-alkoxy; or C^-alkyl optionally substituted with halogen; or a salt thereof with a pharmaceutically acceptable acid or base. 14. A pharmaceutical composition comprising a compound according to claim 13 together with a pharmaceutically acceptable carrier or diluent. 15. A pharmaceutical composition according to claim 14 in the form of an oral dosage unit or parenteral dosage unit. 16. The pharmaceutical composition according to claim 15, wherein said dosage unit comprises from about 1 to about 100 mg of the compound according to claim 13. 17. A method of preparing a compound of formula lb, CHARACTERIZED IN a) reacting a compound of formula Mb, prepared by well known methods, wherein R 1b , R 5b , R 6b , R 7b and R 8b have the meanings defined above, with a N,N-dimethyl amide, preferably dime¬ thylformamide or dimethylacetamide, and POX 3 , wherein X is chlorine or bromine, using Vilsmeyer-Hack conditions, to form a compound of formula lllb, wherein X is chlorine or bromine, and R 1b , R 4b , R 5b , R 6b , R 7b , and R 8 have the meanings defined above; and subsequently b) reacting a compound of formula lllb, wherein X is chlorine or bromine, and R 1b , R 4b , R 5b , R 6b , R 7b , and R 8b have the meanings defined above, with a compound N≡C-CH 2 -Z wherein Z is cyano, nitro, COOR 9b wherein R 9 have the meaning defined above, sulfamoyl unsubstituted or mono or disubstituted with C^-alkyl, or carbamoyl unsubstituted or mono or disubstituted with C^- alkyl; and with an amine as defined for R 2b , preferably morpholine or piperidine, either in the presence or absence of an added strong base, such as sodium hydride, to form a compound of formula IVb, wherein Z, R 1b , R 2b , R 4b , R 5b , R 6b , R 7b , and R 8b have the meanings defined above, and c) reacting a compound of formula IVb, wherein Z b , R 1b , R 2b , R 4 , R 5b , R 6b , R 7b , and R 8b have the meanings defined above, by means of well known chemical reactions transforming the Z b group to hydrogen or to other func¬ tional groups such as acids, esters, amides, amines, or reaction products thereof as described for R 3b , to form a compound of formula lb, wherein R b , R 2b , R 3 , R 4b , R 5b , R 6b , R 7 , and R 8 have the meanings defined above, or d) reacting a compound of formula formula lb, wherein R 1b , R b , R 3b , and R 4b have the meanings defined above, and at least one of R 5b , R 6b , R 7b , or R 8b is H with well known reactive substrates leading to aromatic substitution using the reaction conditions known in the art, to form a compound of formula lb, wherein R 5b , R 6 , R 7b , or R 8 have the meanings defined above provided that at least one of R 5b , R 6 , R 7b , or R 8b is not H.

Inventors: KANSTRUP ANDERS, OLESEN PREBEN H, KANSTRUP, ANDERS, OLESEN, PREBEN, H.
Applicants: KANSTRUP ANDERS, NOVONORDISK AS, OLESEN PREBEN H, KANSTRUP, ANDERS, NOVO NORDISK A/S, OLESEN, PREBEN, H.
Application year: 1996
Main classification: C07D473
IPC codes: A61K31, A61P25, C07D471