NOVEL PEPTIDE AND THERAPEUTIC AGENT

Abstract: A novel peptide represented by formula [I] having allergic and nonallergic inflammation inhibitory, antibacterial, antitumor and wound-healing effects and being useful as an inflammation inhibitor, immunosuppressive agent, antibacterial agent, antitumor agent, wound-healing agent, antiulcer agent, and so forth. The invention also provides therapeutic agents having the abovementioned pharmacological effects.

Description: Technical Field The present invention relates to a novel peptide. The novel peptide of the present invention exhibits allergic and anallergic inflammation inhibitory and wound-healing effects, and it is useful as an inflammation inhibitor, immunosuppressive agent, wound-healing agent, antiulcer agent, and so forth. Accordingly, the present invention also relates to therapeutic agents having the above-mentioned pharmacological effects. Background Art It is known that an extracted mixture which is obtained by extracting with an organic solvent from a culture medium of Actinomyces Streptomyces nobilis (hereinafter referred to as " S . nobilis" ) or a dried product thereof contains a substance having an allergic anti-inflammatory effect (Japanese Laid-open Patent Publication No. 25053/1993). However, the chemical structure of the substance having the above-mentioned pharmacological effect has not been clarified yet. A first object of the present invention is to clarify the structure of the substance and to provide a novel peptide. More than 5,000 kinds of antibiotics produced by microorganism have been reported so far. Among them, streptomycin, actinomycin C, actinomycin D, etc. originating from microorganisms belonging to Streptomyces genus have been reported. In Agric. Diol. Chem., Vol. 54 (3), 791-794 (1990) a depsipeptide named variapeptin is disclosed. This depsipeptide can be obtained by a culture K 2919 which is similar to Streptomyces variabilis and shows antibiotic and cytotoxic activity. Strain K 2919 producing variapeptin was isolated from a soil sample collected in bosque, in Brazil. The taxonomic studies was done by the method adopted by the International Streptomyces Project (ISP). However, this strain was not deposited in the organizations preserving various microorganisms so that this strain is generally not available. Inflammations are classified into anallergic inflammation and allergic inflammation. Non-steroidal anti-inflammatory agents such as aspirin (Nikkei Science, 3 , 70 (1991)) and indomethacin (Ther. Res., 3,1057 (1985)), and steroidal anti-inflammatory agents such as prednisolone are effective against anallergic inflammation represented by carrageenin edema. On the other hand, allergic inflammations are classified into types I-IV. Type I reaction participates in various allergic diseases such as atopic dermatitis, asthma, rhinitis and the like. Type II reaction, type III reaction (immune complex reaction: Arthus reaction) and type IV reaction (cell immunity reaction: delayed type hypersensitive reaction) have been found to play important roles in the crisis and the development of autoimmune diseases such as rheumatoid arthritis and various inflammatory diseases such as hepatitis, nephritis, dermatitis, infectious disease, hemolytic anemia and insulin-dependent diabetes mellitus. Steroid anti-inflammatory agents such as prednisolone are effective against these allergic inflammations. The steroid anti-inflammatory agents which are effective against allergic inflammation have defects in side effects such as drop in phylaxis function, digestive tube disorder, inhibition of hypophysis-adrenal function and drop in osteogenesis. There is a problem that the non-steroid anti-inflammatory agents which are considered to be safer than the steroid agents have very low inhibitory effects on allergic inflammation. Accordingly, an anti-inflammatory agent which is highly effective and less toxic is desired. In view of the above-mentioned points, a fourth object of the present invention is to provide a novel anti-inflammatory substance which is effective against allergic inflammation and an allergic inflammation and useful. Wounds are injuries of surface tissue such as surgical incisions, an digestive tube ulcers, burns, lacerated wounds and skin ulcers (such as bedsores). A usual treatment for wounds is to give first aid to an injured sites and then to wait for the injured sites to heal by natural recuperative power of a living body. However, recovery of the injured sites takes a long period of time and patient's pains such as aches are by no means easy matter. Accordingly, it is desired to promote the healing positively and directly without relying on spontaneous recovery. It is considered that the healing of wounds generally depends on the formation of new connective tissue and epithelial tissue by cell proliferation and that a medicine stimulating or promoting the cell differentiation process and the cell proliferation process participating in the healing of wounds is effective. An extract from blood of young calves (Solcoseryl) (Pharmacometrics, 22 , 565-579 (1981)), lysozyme chloride, etc. have so far been reported as substance exhibiting promoting effects on the healing of wounds. However, since wounds such as injuries of surface tissue are generally intractable, any existing medicines hardly exhibit sufficient clinical effects on the wounds. In view of the above-mentioned points, a fifth object of the present invention is to provide a more useful novel wound-healing substance. Disclosure of the Invention The present invention provides a novel peptide represented by the formula [I] (hereinafter referred to as "the peptide of the invention"). The peptide of the invention is obtained, for example, by purifying an extract which is extracted with an organic solvent from a culture medium obtained by culturing Actinomyces S. nobilis , a dried product of the medium or the cultured bacterium by various column chromatography, and by recrystalizing a column chromatography fraction containing the desired product. Actinomyces S. nobilis producing the peptide of the invention is available from some public preservation organizations. There can be used, for example, a preserved bacterium of the Institute of Physical and Chemical Research (JCM4274) (also preserved under ATCC19252 in the United States and under CBS198.65 in the Netherlands), etc. Actinomyces S. nobilis is cultured using a medium containing proper nutrition. In case of liquid culture, there can be preferably used an aqueous solution containing one or some kinds of sugars such as glucose, proteins such as peptone and a malt extract, vitamins, nucleic acids, amino acids and complex carbohydrates as components of the medium. A representative example of the medium is a type of starch-ammonium liquid medium (containing soluble starch, K 2 HPO 4 and NH 4 Cl). The pH of the liquid medium is preferably 2 to 9 and the culture temperature is preferably 15 to 42 °C. The culture period of the liquid culture is preferably 1 to 14 days. The peptide of the invention is extracted with a solvent from the culture medium of S. nobilis obtained in such manner, the dried product thereof or the bacterium itself. The extract of the culture medium of S. nobilis , the dried product thereof or the bacterium itself may be treated with ammonium sulfate and the obtained precipitate may be extracted with a solvent. In treating with ammonium sulfate, ammonium sulfate to be added to the extract of the culture medium, the dried product thereof or the bacterium itself may be both in solid and in solution thereof. The amount of ammonium sulfate to be added is preferably in the range of 30 to 90% by weight of the saturated concentration. The treatment time is preferably in the range of 30 minutes to 5 hours, but it is not limited thereto. In centrifuging to obtain the precipitate, the centrifugal force is preferably 3,000 G to 20,000 G and the centrifugation time is preferably 2 to 60 minutes. Organic solvents are preferable as the solvents used for the extraction. Representative examples of the organic solvents are esters such as ethyl acetate, alcohols such as methanol, ethanol and propanol, ethers such as ethyl ether and dioxane, ketones such as acetone and methyl ethyl ketone, dichloromethane, chloroform, etc. However, usable solvents are not limited to them. A mixture of the above-mentioned solvents can be also used. Preferred solvents are ethyl acetate, dichloromethane, acetone, etc. The extraction time varies with kind of the solvent, extraction temperature, etc., but it is preferably in the range of 3 to 120 minutes. The liquid may be both allowed to stand and stirred during the extraction. The extraction operation is preferably repeated several times with respect to one sample. The extraction temperature is not particularly limited. Next column chromatography for the solvent extract is described. As a filler for column chromatography, ODS (octadecylsilanes such as octadecyldimethylchlorosilane) is preferable. The filler content is not particularly limited but it is preferable to charge the filler having a weight 10 to 500 times as much as the solvent extract to be charged. In charging the solvent extract into a column, it is preferable to first make the solvent extract adsorb on the ODS. The amount of the ODS is not particularly limited either, but it is preferable to make the solvent extract adsorb on ODS having a weight 0.5 to 20 times as much as the extract, to suspend the ODS adsorbing the extract in a small amount of a solvent and to charge the suspension into the column. The elution solvent is not particularly limited either, but it is preferable to use solvents having polarity between a 70% aqueous solution containing a mixed solvent of methanol-acetonitrile (1:1) and methanol-acetonitrile-water (19:19:2). Next purification of the peptide of the invention by recrystalization is described. Solvents used for recrystalization are not particularly limited so long as they dissolve the peptide of the invention, but methanol, ethanol, etc. are preferable. As a method of recrystalization, a column elution fraction containing the desired substance may be dissolved in a small amount of a solvent upon heating followed by cooling the obtained solution gradually to recrystalize the substance. Alternatively, the column elution fraction containing the substance may be also dissolved in a solvent which easily dissolves the substance, followed by adding a liquid which hardly dissolves the substance (for example, water) to the solution gradually to recrystalize the substance. As demonstrated by the pharmacological test mentioned below, the peptide of the invention exhibits allergic and anallergic inflammation inhibitory effects and a wound-healing effect, and it is useful as an inflammation inhibitor, immunosuppressive agent, wound-healing agent, antiulcer agent, and so forth. To prepare the above-mentioned therapeutic agents, the peptide of the invention is usually formulated into a preparation composition with a carrier for preparation. Examples of the carriers are a filler, a disintegrator, an extender, a binder, a colorant, a flavor, a pH adjustor, a solubilizer, a suspending agent, a buffer agent, a stabilizer, a preservative, a humectant, a surfactant, a lubricant, an excipient, an antioxidant, a dispersant, a propellant, a dissolvent and a solubilizing agent which are usually used to prepare medicines according to dosage forms. The obtained peptide of the invention can be also used in a liquid preparation form by selecting a suitable solvent. Examples of dosage forms of the therapeutic agents prepared using the peptide of the invention are tablet, pill, solution for drinking, powder, suspension, emulsion, granule, extract, subtilized granule, syrup, infusion, decoction, eye drop, troche, poultice, liniment, lotion, ophthalic ointment, plaster, capsule, suppository, injection (e. g., liquid preparation and suspension), adhesive tape, ointment, jelly, pasta, inhalant, cream, spray, collunarium and aerosol other than the above-mentioned liquid preparation. The amount of the peptide of the invention to be contained in the therapeutic agents is not particularly limited and appropriately selected over a wide range. Preferred amount of the peptide is in the range of 10 -7 to 10% by weight in the therapeutic agents. The therapeutic agents obtained from the peptide of the invention are administered by methods according to various forms when used. For example, external preparations are directly sprayed, applied or spread to required sites such as skin and mucosa. Tablets, pills, solutions for drinking, suspensions, emulsions, granules and capsules are administered orally. Injections are administered intravenously, intramuscularly, intracutaneously, subcutaneously, intraarticularly and intraperitoneally. Suppositories are administered intrarectally. The dosage of the therapeutic agents obtained from the peptide of the invention is appropriately adjusted depending on purpose, symptoms, etc. The usual daily dosage is in the range of about 10 pg/kg to about 10 mg/kg as the peptide of the invention. Of course, the above-mentioned preparation composition can be administered in one to four divided doses per day. Brief Description of Drawings Fig. 1 is a graph showing leaking pigment amounts in rat 48-hour homologous PCA reaction by administering intraperitoneally 0.5 mg/kg of the peptide of the invention, 20 mg/kg of prednisolone and a control thereof respectively. Fig. 2 is a graph showing leaking pigment amounts in rat 48-hour homologous PCA reaction by administering subcutaneously 0.5 mg/kg of the peptide of the invention, 20 mg/kg of prednisolone and a control thereof respectively. Fig. 3 is a graph showing leaking pigment amounts in rat 4-hour heterologous PCA reaction by administering intraperitoneally 0.5 mg/kg of the peptide of the invention, 20 mg/kg of prednisolone and a control thereof respectively. Fig. 4 is a graph showing leaking pigment amounts in rat 4-hour heterologous PCA reaction by administering subcutaneously 0.5 mg/kg of the peptide of the invention, 20 mg/kg of prednisolone and a control thereof respectively. Fig. 5 is a graph showing degrees of erythema and skin weights in rat tuberculin reaction by administering intraperitoneally 0.5 mg/kg of the peptide of the invention, 50 mg/kg of prednisolone and a control thereof respectively. Fig. 6 is a graph showing right hind footpad edema volume in rat carrageenin edema by administering intraperitoneally 0.5 mg/kg of the peptide of the invention, 100 mg/kg of aspirin, 5 mg/kg of prednisolone and a control thereof respectively. Fig. 7 is a graph showing footpad edma amounts in rat adjuvant arthritis by administering intraperitoneally 0.5 mg/kg of the peptide of the invention, 10 mg/kg of prednisolone and a control thereof respectively. Fig. 8 is a graph showing a relationship between concentrations of the peptide of the invention, a control and a reference medicine, and wet weights of granulation. Fig. 9 is a graph showing a relationship between the peptide of the invention, a control and a reference medicine, and tensile strength of wound. Fig. 10 is a graph showing a relationship between days after forming defects and a defect area with respect to ointment containing the peptide of the invention and control ointment. Fig. 11 is a graph showing a relationship between days after forming defects and a defect area with respect to ointment containing the peptide of the invention and reference ointment. Best Mode for Carrying out the Invention Example 1 Actinomyces S. nobilis (JCM4274) which had been obtained from the Institute of Physical and Chemical Research was shaking-cultured (pre-preculture) for 40 hours in 100 ml of a starch-ammonium medium to which a 0.2% yeast extract had been added. Then 3 liters of the medium was inoculated with 60 ml of the pre-precultured bacterium liquid, and the mixture was shaking-cultured (seed culture) for 25 hours. In addition, 285 liters of a starch-ammonium medium (containing 1 g of soluble starch, 0.05 g of dipotassium hydrogenphosphate and 0.05 g of ammonium chloride in 100 ml of distilled water) was inoculated with the whole amount of the seed-cultured medium, and the mixture was shaking-cultured at about 30°C for 8 days. The culture medium was centrifuged. Ammonium sulfate was added to 250 liters of the obtained supernatant at a rate of 662 g per 1 liter of the supernatant and the mixture was stirred for 1 hour. The mixture was then continuously centrifuged at 20,000 G(flow rate: 360 to 500 liters/hr) to give about 12 kg of a precipitate. To 1.2 kg of the precipitate obtained by the above-mentioned method was added 3.6 liters of water and the mixture was stirred at 80 °C for 30 minutes. An equal amount of ethyl acetate was added thereto, and the mixture was shaked for 10 minutes, followed by centrifuging the whole at 5,000 G for 10 minutes. After separating the ethyl acetate layer, the aqueous layer was subjected to this operation several times to give a solvent extract. Then the solvent extract obtained by the above-mentioned method was adsorbed on 25 g of an ODS (octadecyldimethylchlorosilane) sorbent. The sorbent adsorbing the extract was charged on an ODS sorbent in a column having a diameter of 3.2 cm to prepare an ODS column. This column had been filled with 275 g of the sorbent, in advance. The extract was purified with the ODS column under the following condition. The following elution solvents were made to flow in turn at the rate of 10.5 ml/min: a) 1.5 liters of methanol-acetonitrile-water (7:7:6), b) 1.2 liters of methanol-acetonitrile-water (8:8:4), c) 150 ml of methanol-acetonitrile-water (9:9:2), d) 1 liter of methanol-acetonitrile-water (19:19:2) and c) 1 liter of methanol. Each eluate was collected every time the composition of the elution solvent was changed. In particular, the elution fraction of methanol-acetonitrile-water (19:19:2) was appropriately fractionated little by little (every 2 minutes) with a fraction collector. Each elution fraction was analyzed with a high-performance liquid chromatograph (manufactured by Hitachi, Ltd., pump: L-6000L-6200, detector: L-3000, column oven: 655A-52) provided with a column (manufactured by Tosoh Corp., ODS-80TM, 4.6 mm in inner diameter and 25.0 cm in length) under the following conditions; detection wave length = 210 nm, column temperature = 40 °C and flow rate = 1 ml/min, using eluents of water-acetonitrile-methanol (6:7:7 (0 minute) to 0:1:1 (30 minutes)). Only fractions containing a peak having a retention time of 18 to 20 minutes were collected as the same fraction (100 mg) among the above-mentioned fractions. The collected fraction was repeatedly recrystalized from methanol-water to give 45 mg of needles. The structure of this substance was determined to be that represented by formula [I] by various instrumental analysis data. Example 2 S. nobilis was cultured in the same manner as in Example 1. To 215 g (wet weight) of the obtained bacterium was added 2.15 liters of dichloromethane and the mixture was subjected to ultrasonication for 30 minutes. To the mixture was further added 8.6 liters of dichloromethane and the whole was stirred at room temperature for 1 hour. After filtering off the bacteria, the obtained filtrate was concentrated to dryness. The dried product of the extract was dissolved in a small amount of hexane and the hexane phase was extracted with methanol three times. The substance being insoluble in hexane and the methanol extract were dissolved in methanol again. Next the solvent extract obtained by the above-mentioned method was adsorbed on 28 g of an ODS sorbent. About 30 g of the sorbent adsorbing the extract was charged on an ODS sorbent in a column having a diameter of 3.2 cm to prepare an ODS column. This column had been filled with 275 g of the sorbent, in advance. The extract was purified with the ODS column under the following condition. The following elution solvents were made to flow in turn at the rate of 10.5 ml/min: a) 1.5 liters of methanol-acetonitrile-water (7:7:6), b) 1.2 liters of methanol-acetonitrile-water (8:8:4), c) 200 ml of methanol-acetonitrile-water (9:9:2), d) 1 liter of methanol-acetonitrile-water (19:19:2) and e) 500 ml of methanol. Each eluate was collected every time the composition of the elution solvent was changed. In particular, the elution fraction of methanol-acetonitrile-water (19:19:2) was appropriately fractionated little by little (every 2 minutes) with a fraction collector. Each elution fraction was analyzed with a high-performance liquid chromatograph (manufactured by Hitachi, Ltd., pump: L-6000L-6200, detector: L-3000, column oven: 655A-52) provided with a column (manufactured by Tosoh Corp., ODS-80TM, 4.6 mm in inner diameter and 25.0 cm in length) under the following conditions; detection wave length = 210 nm, column temperature = 40 °C and flow rate = 1 ml/min, using eluents of water-acetonitrile-methanol (6:7:7 (0 minute) to 0:1:1 (30 minutes)). Only fractions containing a peak having a retention time of 18 to 20 minutes were collected as the same fraction (840 mg) among the above-mentioned fractions. The collected fraction was repeatedly recrystalized from methanol-water to give 330 mg of the peptide of the invention as needles. The structure of this substance was determined to be that represented by formula [I] by various instrumental analysis data in the same manner as in Example 1. Structure analysis data The instrumental analysis data of the substances obtained in Examples 1 and 2 are shown below. 1. MS ESI-MS : m/z=913.6 (M+H-H 2 O) + , 931.6 (M+H) + , 953.6 (M+Na) + HRFAB-MS Found : m/z=913.5079 (M+H-H 2 O) + , m/z=913, 953, 931 (The peak of 913 is main and the peak of 931 is very small.) Calcd for : C 45 H 69 N 8 O 12 m/z=913.5053 2. IR IR : 3,400 cm -1 : -OH, -NH, 2,900 cm -1 : alkyl groups, 1,750 cm -1 -C(=O)-O-, 1,650 cm -1 : -C(=O)-NH- 3. Amino acid analysis D-serine, L-alanine and D-N-methylphenylalanine were detected as hydrolysis products. 4. Compositional formula The compositional formula of the novel peptide was found to be C 45 H 70 N 8 O 13 by the data of mass spectrometry and the following NMR. 5. NMR a) Partial structure A As a result of measurement of HMBC spectrum, a long-range correlation was observed between carbonyl carbon (173.6 ppm) of alanine (Ala) and CH 3 (3.04 ppm) of N-CH 3 -phenylalanine (Phe). Accordingly, the partial structure A was presumed. b) Partial structures B and C Two components having a similar spin system were presumed by 1 H- 1 H COSY, HOHAHA. i) NH (4.38 ppm) CH 2 (46.1, 2.89, 3.15 ppm) CH 2 (21.3, 1.45, 1.63 ppm) CH 2 (24.2, 1.93, 2.25 ppm) CH (51.7, 4.90 ppm) ii) NH (3.93 ppm) CH 2 (47.9, 2.56, 3.30 ppm) CH 2 (21.5, 1.55, 1.66 ppm) CH 2 (24.4, 1.67, 2.58 ppm) CH (52.5, 5.18 ppm) Since the 13 C shifts of the components are very close, they are considered to be based on the same structure (residue). In addition, since the CH 2 protons are unequivalent, the components can be assumed to have cyclic structure.Since the shifts agree with those of piperazic acid (Pip) contained in Monamycin, the components were assumed to be Pip (the left part of the partial structure B and the partial structure C). α β γ δ Pip 1) 49.8 24.1 21.0 47.0 (CDCl 3 solution) Found 52-53 24-25 21 46-48 (CDCl 3 solution) Furthermore, since a long-range correlation was observed between NH of 6.40 ppm of serine (Ser) and carbonyl of 168.9 ppm of Pip, the partial structure B was assumed. 1) C. H. Hassall, W. A. Thomas, M. C. Moschidis, J. Chem. Soc., Perkin Trans. I, 1977, 2371 (1977) c) Partial structure D The partial structure represented by the formula of (CH 3 ) 2 -CH-CH-CH-NH- was assumed from 1 H- 1 H spin coupling. The partial structure was confirmed by long-range 1 H- 13 Ccoupling. The structure of β-OH-leucine (Leu) was confirmed by spin coupling between carbonyl of 170.6 ppm and CH of 4.88 ppm. β-OH-Leu was assumed to form an ester bond at β position thereof from CH of 5.46 ppm.The structure of the side chain part (left) of the partial structure D was confirmed by results of measurements of 1 H- 1 H spin coupling, long-range 1 H- 13 C coupling and NOE (Nuclear Overhauser Effect).The partial structure D was assumed by the fact that a long-range correlation is observed among CH of 4.88 ppm and NH of 8.34 ppm of β-OH-Leu, and carbonyl of 177 ppm of the side chain part from long-range 1 H- 13 C coupling. d) Chemical structure of the novel peptide The partial structures B and D were found to form an ester bond between Ser and β-OH-Leu by the fact that a long-range correlation is observed between carbonyl of 170.2 ppm of Ser and CH of 5.46 ppm of β-OH-Leu from long-range 1 H- 13 C coupling. The partial structure C was found to bond to the carbonyl group of 170.6 ppm of β-OH-Leu by the fact that NOE is observed between CH of 5.46 ppm of β-OH-Leu and NH of 4.38 ppm of Pip (partial structure C), between NH of 8.34 ppm of β-OH-Leu and CH 2 of 2.89 ppm of Pip (partial structure C), and between NH of 8.34 ppm and CH of 4.90 ppm of Pip (partial structure C). Since the method of bonding the remaining partial structure A was one way, the chemical structure of the novel peptide was determined.The above-mentioned structure supported to be correct by the fact that NOE is also observed between NH of 6.40 ppm of Ser and CH 3 of 3.04 ppm of N-CH 3 -Phe. e) The results of measurements of NMR are shown below. Tables 1 and 2 show 13 C shifts, carbon types and directly coupling 1 H. Tables 3-5 show 1 H shifts, spin-coupling 1 H and 13 C, and long-range-coupling 13 C. Tables 6 and 7 show results of measurements of NOE. Tables 8 - 11 show assignments of 13 C and 1 H. 13 C Shift, carbon type and coupling 1 H Carbon No. 13 C Shift Type Coupling 1 H 1 177.0 >C=O - 2 174.3 >C=O - 3 173.6 >C=O - 4 171.9 >C=O - 5 170.6 >C=O - 6 170.2 >C=O - 7 168.9 >C=O - 8 136.6 >C= - 9 129.4 2 · -CH= 7.25 10 128.3 2 · -CH= 7.26 11 126.7 -CH= 7.20 12 99.2 >C< - 13 78.9 >CH- 5.46 14 76.8 >C< - 15 71.6 >CH- 3.74 16 60.7 -CH2- 3.49, 4.54 17 55.0 >CH- 4.88 18 52.5 >CH- 4.75 19 52.5 >CH- 5.18 20 52.0 >CH- 6.47 21 51.7 >CH- 4.90 22 50.6 >CH- 5.03 23 47.9 -CH2- 2.56, 3.30 24 46.1 -CH2- 2.89, 3.15 25 40.9 -CH2- 0.95, 1.08 26 39.8 >CH- 1.10 27 33.9 -CH2- 3.02, 3.11 28 29.4 -CH3 3.04 29 29.3 >CH- 1.93 30 27.8 -CH2- 1.65, 1.74 3 1 24.7 >CH- 1.66 32 24.4 -CH2- 1.67, 2.58 33 24.18 -CH2- 1.93, 2.25 34 24.16 -CH3 0.893 35 24.13 -CH2- 1.75, 1.44 36 21.5 -CH2- 1.66, 1.55 37 21.4 -CH3 0.83 38 21.3 -CH2- 1.45, 1.63 39 20.0 -CH3 1.375 40 19.7 -CH3 0.890 41 19.3 -CH3 1.02 42 14.9 -CH3 1.01 43 13.0 -CH3 1.14 Result of measurement of NOE 1 H Shift (J) In residue Between residue 1 9.62(s) 2 8.341(d;10.6) 9 21B,12 3 7.26 4 7.25 6 5 7.20 6 6.473(d,d;7.0,9.5) 22A 11 7 6.46(br-s) 8 6.398(d;7.9) 14, 15B, 18 10,23 9 5.463(d,d;9.6,1.8) 2, 13, 27, 40 16 10 5.179(d;d) 25A 8 11 5.027(q;7.2) 34 6,23 12 4.90 26A;26B 2 13 4.88 9, 38 14 4.751(d,d;8.4,3.7) 8, 15A, 15B 40 15A 4.546(d;11.7) 14, 15B 23 15B 3.494 8, 15A, 14 16 4,378(d,d) 21A, 32B 9 17 4.28(br-s) 18 3.926(d;11.4) 20A 8 19 3.740(d,q;9.5,6.2) 37 35A, 35B 20A 3.300(d;13.2) 208, 31, 18 20B 2.56 20A 21A 3.15(d) 16, 21B 21B 2.89(q;13) 21A 2 22A 3.114(d,d;14.1,7.0) 6,3 or 4, 23 22B 3.02(d,d;14,1,9.5) 3 or 4, 23 23 3.040(s) 3 or 4 11,34 24 3.04 25A 2.58 10, 20A, 31B 25B 1.67 26A 2.25(d) 26B, 12 26B 1.93 26A, 12 27 1.93(m) 9, 38, 40 28A 1.75 28B, 35A 28B 1.44 28A 29A 1.74 29B 1.65 30 1.66 31A 1.66 31B 1.55 20A 32A 1.63 32B 1.45 33 1.375(s) 34 1.141(d;7.0) 11 23 35A 1.07 35B, 19 35B 0.96 35A, 19 36 1.09 37 1.022(d;6.2) 19 38 1.007(d;7.0) 27, 13 39 0.893 (d;6.2) 40 0,890(d;7.3) 9 41 0.827(d;6.6) A and B of the same number represent unequivalent CH 2 . Assignment of 13 C and 1 H peaks δC δH Ser 1(CO) 170.2 - 2(CH) 52.5 4.75 3(CH2) 60.7 3.49, 4.54 3-OH - 2-NH - 6.40 Pip 4(CO) 168.9 - 5(CH) 52.5 5.18 6CH2) 24.4 1.67, 2.58 7(CH2) 21.5 1.55, 1.66 8(CH2) 47.9 2.56, 3.30 8-NH - 3.93 N-Me-Phe 9(CO) 171.9 - 10(CH) 52.0 6.47 11(CH2) 33.9 3.02, 3.11 12(C) 136.6 - 13,17(CH) 129.4 7.25 14,16(CH) 128.3 7.26 15(CH) 126.7 7.20 18(CH3) 29.4 3.04 N-OH-Ala 19(CO) 173.6 - 20(CH) 50.6 5.03 21(CH3) 13.0 1.14 N-OH - 9.62 Pip 22(CO) 174,3 - 23(CH) 51.7 4.90 24(CH2) 24.2 1.93, 2.25 25(CH2) 21.3 1.45, 1.63 26(CH2) 46.1 2.89, 3.15 26-NH - - 4.38 3-OH-Leu 27(CO) 170.6 - 28(CH) 55.0 4.88 29(CH) 78.9 5.46 30(CH) 29.4 1.93 31(CH3) 19.7 0.890 32(CH3) 14.9 1.01 28-NH - 8.34 Side chain 33(CO) 177.0 - 34(C) 76.8 - 35(C) 99.2 - 36(CH2) 27.8 1.65, 1.74 37(CH2) 24.1 1.44, 1.75 38(CH) 39.8 1.10 39(CH) 71.6 3.74 40(CH3) 20.0 1.375 41(CH2) 40.9 0.95, 1.08 42(CH) 24.7 1.66 43(CH3) 24.1 0.893 44(CH3) 21.4 0.83 45(CH3) 19.3 1.02 Others (OH) 6.46, 4.28, 3.04 Formulation Formulation 1 (ointment) peptide of the invention 1 mg Plastibase (manufactured by Taisho Pharmaceutical Co., Ltd.) 1 g After 1 mg of the peptide of the invention was divided finely using a mortar, 1 g of Plastibase was added to the peptide. They are mixed on the mortar sufficiently to prepare an ointment. Formulation 2 (ointment) peptide of the invention 0.2 mg methanol 12.5 µl Plastibase (manufactured by Taisho Pharmaceutical Co., Ltd.) 1 g After 0.8 mg of the peptide of the invention was dissolved in 50 µl of methanol, 12.5 µl of the obtained methanol containing the peptide of the invention was mixed with 1 g of Plastibase on the mortar sufficiently to prepare an ointment. Formulation 3 (liquid preparation) peptide of the invention 0.5 mg DMSO 0.1 ml 5% by weight of aqueous gum arabic solution 1.9 ml After 0.1 ml of DMSO was added to 0.5 mg of the peptide of the invention to dissolve the peptide, 0.1 ml of the obtained DMSO solution containing the peptide of the invention was added to 1.9 ml of 5% by weight of an aqueous gum arabic solution little by little while stirring to prepare a liquid preparation suspended homogeneously. Formulation 4 (liquid preparation) peptide of the invention 0.5 mg physiological saline 2 ml A small amount of physiological saline was added to 0.5 mg of the peptide of the invention and the obtained mixture was subjected to ultrasonication to prepare a homogeneous suspension. Physiological saline was added to the suspension to prepare 2 ml of a liquid preparation. Formulation 5 (liquid preparation) peptide of the invention 25 mg ethanol 2.5 ml polyoxyethylene hydrogenated castor oil 60 1 g physiological saline 96.5 ml After 25 mg of the peptide of the invention was dissolved in 2.5 ml of ethanol, 1 g of polyoxyethylene hardened castor oil 60 was added thereto. The obtained solution was added to 96.5 ml of physiological saline little by little while stirring to prepare a liquid preparation. Formulation 6 (liquid preparation) peptide of the invention 10 mg macrogol 400 50 ml physiological saline q. s. After 10 mg of the peptide of the invention was dissolved in 50 ml of macrogol, physiological saline was added thereto to prepare 100 ml of a liquid preparation. Pharmacological test Test 1 is given as a test showing an antimicrobial activity of the peptide of the invention. Tests 3-7 are given as tests showing an antiinflammation activity. Tests 8-10 are given as tests showing a wound-healing activity. Test 11 is given as an acute toxicity test of the peptide of the invention. Test 3 Effect on Type I allergic inflammation i) Preparation of rat anti-2,4-dinitrobenzenesulfonic acid-Ascaris suum extract (DNP-As) serum DNP-As was prepared according to the method of Tada and Okumura (Journal of Immunology, 106 ,1002 (1971)). An Ascaris suum extract was prepared according to the method of Strejan and Campbell (Journal of Immunology, 98, 893 (1967)) and was bonded to 2,4-dinitrobenzenesulfonic acid (DNP) according to the method of Eisen et al. (Journal of American Chemical Society, 75 , 4583 (1953)). One miligram of DNP-As was dissolved in 1 ml of physiological saline on which 10 10 of dead Bordetella pertussis were floated and the solution was injected subcutaneously into four footpads of a female rat having weight of about 200 g. Five days after, 0.5 mg of DNP-As was dissolved in 0.5 ml of physiological saline and the solution was injected intramusclarly into the right and left back. Eight days after the first injection, the blood was collected from aorta abdominalis. The serum was separated and used as a rat anti-DNP-As serum. The potency of the antiserum in rat 48-hour homologous PCA reaction was 1 :512. ii) Effect on rat 48-hour homologous PCA reaction (Type I allergic cutaneous reaction) The peptide of the invention was suspended in a solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of an aqueous gum arabic solution so that the concentration of the peptide was 0.25 mg/ml. Prednisolone was suspended in the solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of the aqueous gum arabic solution so that the concentration of prednisolone was 10 mg/ml for a control experiment. The suspensions obtained in this manner were used as test liquids. Male Wister rats having weight of 120 to 200 g were used as test animals. First, 0.05 ml of an injection obtained by diluting the above-mentioned anti-DNP-As serum 20 times with physiological saline was injected intracutaneously into the back of the rat and thereby the rat was sensitized with the antiserum. Fourty-eight hours after the administration of the antiserum, 2.5 ml/kg of 0.5 wt% of Evans' Blue physiological saline containing 2 mg/ml of DNP-As as the corresponding antigen was administered intraveneously to induce PCA reaction. With respect to the rats in the group to which the peptide of the invention was administered, 2 ml/kg of the test liquid containing the peptide of the invention (the peptide of the invention: 0.5 mg/kg) was administered intraperitoneally or subcutaneously 24 hours before the induction. With respect to the rats in the group to which prednisolone was administered, 2 ml/kg of the test liquid containing prednisolone (prednisolone: 20 mg/kg) was administered intraperitoneally or subcutaneously 3 hours before the induction. The leaking pigment at the site where the intracutaneous reaction was caused was extracted and the amount was determined according to the method of Harada et al. (J. Pharm. Pharmacol., 23, 218 (1971)) as follows. The rat was subjected to euthanasia under ether anesthesia one hour after the antigen injection. The skin at PCA reaction site of the rat was incised, minced and immersed in a mixed solution of 3 volumes of a 0.3% (w/v) aqueous sodium sulfate solution and 7 volumes of acetone for 24 hours. The leaking pigment amount was determined. The obtained value represents the leaking pigment amount (µ g) per the site where the antiserum was injected. The same procedure as mentioned above was repeated with the exception of using the above-mentioned aqueous gum arabic solution containing dimethyl sulfoxide and no drugs as a control of the test to determine the leaking pigment amount. Each test was carried out using five rats. The leaking pigment amount (µg/site) was expressed in the average of the values obtained with respect to the rats. The test results of the intraperitioneal administration are shown in Fig. 1. The test results of the subcutaneous administration are shown in Fig. 2. As apparent from these figures, the group to which the test liquid containing the peptide of the invention had been administered exhibited a remarkable decrease of the pigment amount leaking to the skin at rat 48-hour homologous PCA reaction site compared with the control group and thereby indicated a high type I allergic inflammation inhibitory activity. The group to which the test liquid containing the peptide of the invention had been administered exhibited such a remarkable decrease of the pigment amount leaking to the skin at rat 48-hour homologous PCA reaction site compared with the control group, in a smaller dose than that of the test liquid containing prednisolone. Namely, the peptide obtained by the present invention has a higher type I allergic inflammation inhibitory activity than prednisolone. Test 4 Effect on type III allergic inflammation i) Preparation of rabbit antiovalbumin serum A rabbit antiovalbumin scrum was prepared according to the method of Eda et al. (Folia pharmacologica Japonica, 66 , 237 (1970)) by the following method. An antigen solution comprising an emulsion was prepared by mixing equal amounts of 2 mg/ml of a solution of ovalbumin (manufactured by Sigma Chemical Co.) dissolved in physiological saline and complete Freund's adjuvant (manufactured by Difco Co., Ltd.). Into right and left gluteal regions of a male New Zealand white house rabbit having weight of 3 kg was injected 0.5 ml of the antigen solution intramuscularly four times every one week. The blood was collected from carotid artery of the rabbit 7 days after the final injection and only the serum was separated as the rabbit antiovalbumin serum. The potency of the antiserum in rat 4-hour heterologous passive cutaneous anaphylaxis (4-hour heterologous PCA) reaction was 1 : 32. ii) Effect on rat 4-hour heterologous PCA reaction (Type III allergic dermoreaction) The peptide of the invention was suspended in a solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of an aqueous gum arabic solution so that the final concentration of the peptide was 0.25 mg/ml. Prednisolone was suspended in the solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of the aqueous gum arabic solution so that the final concentration of prednisolone was 10 mg/ml for a control experiment. The suspensions obtained in this manner were used as test liquids. Male Wister rats having weight of 120 to 200 g were used as test animals. Into the dorsum of the rat was injected intracutaneously 0.05 ml of an injection obtained by diluting the above-mentioned rabbit antiovalbumin serum four times with physiological saline and thereby the rat was sensitized with the antiserum. Four hours after the administration of the antiserum, 2.5 ml/kg of 0.5 wt% Evans Blue physiological saline containing 2 mg/ml of ovalbumin as the corresponding antigen was injected intraveneously to induce heterologous PCA reaction for 4 hours. With respect to the rats in the group to which the peptide of the invention was administered, 2 ml/kg of the test liquid containing the peptide of the invention (the peptide of the invention: 0.5 mg/kg) was administered intraperitoneally or subcutaneously 24 hours before the induction. With respect to the rats in the group to which prednisolone was administered, 2 ml/kg of the test liquid containing prednisolone (prednisolone: 20 mg/kg) was administered intraperitoneally or subcutaneously 3 hours before the induction. The leaking pigment at the site where the intracutaneous reaction was caused was extracted and the amount was determined according to the method of Harada et al. (J. Pharm. Pharmacol., 23, 218 (1971)) as follows. The rat was subjected to euthanasia under ether anesthesia one hour after the antigen injection. The skin at 4-hour heterologous PCA reaction site of the rat was narrowly cut off and was immersed in a mixed solution of 3 volumes of a 0.3% (w/v) aqueous sodium sulfate solution and 7 volumes of acetone for 24 hours. The leaking pigment amount was determined. The obtained value represents the leaking pigment amount (µg) per the site where the rabbit antiovalbumin serum was injected. The same procedure as mentioned above was repeated with the exception of using the above-mentioned aqueous gum arabic solution containing dimethyl sulfoxide and no drugs as a control of the test to determine the leaking pigment amount. Each test was carried out using five rats. The leaking pigment amount (µ g/site) was expressed in the average of the values obtained with respect to the rats. The test results of the intraperitoneal administration are shown in Fig. 3. The test results of the subcutaneous administration are shown in Fig. 4. As apparent from these figures, the group to which the test liquid containing the peptide of the invention had been administered exhibited a remarkable decrease of the pigment amount leaking to the skin at rat 4-hour heterologous PCA reaction site compared with the control group and thereby indicated a high allergic inflammation inhibitory activity. The group to which the test liquid containing the peptide of the invention had been administered exhibited such a remarkable decrease of the pigment amount leaking to the skin at rat 4-hour heterologous PCA reaction site compared with the control group, in a smaller dose than that of the test liquid containing prednisolone. Namely, the peptide of the invention has a higher type III allergic inflammation inhibitory activity than prednisolone. Test 5 Effect on type IV allergic inflammation An effect of the peptide of the invention on rat type IV allergic inflammation (tuberculin reaction) was examined by the following method. First, the peptide of the invention was suspended in a solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of an aqueous gum arabic solution so that the final concentration of the peptide was 0.25 mg/ml. Prednisolone was suspended in the solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of the aqueous gum arabic solution so that the concentration of prednisolone was 25 mg/ml for a control experiment. The suspensions obtained in this manner were used as test liquids. Male Wister rats having weight of 170 to 250 g were used as test animals. Tuberculin reaction was induced according to the method of Kuriyama et al. (Folia pharmacologica Japonica, 94, 113-118 (1989)) provided that 2 ml/kg of the test liquid (the peptide of the invention: 0.5 mg/kg, prednisolone: 50 mg/kg) was administered intraperitoneally one hour before the induction. Twenty-four hours after the induction, the degree of erythema was measured according to the Draize's criterion. A reaction site was stamped out with a punch having a diameter of 1.8 cm and the skin weight was measured. The same procedure as mentioned above was repeated with the exception of using the above-mentioned aqueous gum arabic solution containing dimethyl sulfoxide and no drugs instead of the above-mentioned test liquid as a control of the test. Each test was carried out using four rats. The degree of erythema and the skin weight were expressed in the averages of the values obtained with respect to the rats. The test results are shown in Fig. 5. As apparent from this figure, the group to which the test liquid containing the peptide of the invention had been administered exhibited a remarkable inhibition of the erythema and a remarkable decrease of the skin weight compared with the control group and the effects were higher than those of the group to which prednisolone had been administered. Namely, the peptide of the invention has a higher inhibitory activity on type IV allergic inflammation (tuberculin reaction) being immune inflammation than prednisolone. Test 6 Effect on anallergic inflammation An effect of the peptide of the invention on rat carrageenin footpad edema reaction was examined by the following method. First, the peptide of the invention was suspended in a solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of an aqueous gum arabic solution so that the final concentration of the peptide was 0.25 mg/ml. Aspirin and prednisolone were individually suspended in the solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of the aqueous gum arabic solution so that the final concentrations of aspirin and prednisolone were 50 mg/ml and 2.5 mg/ml respectively for control experiments. The suspensions obtained in this manner were used as test liquids. Male Wister rats having weight of 120 to 200 g were used as test animals. The volume of a right hind leg of the rat was measured with a plethysmometer. Then 0.1 ml of 1% (w/v) carrageenin solution in physiological saline was injected intracutaneously into the right hind footpad of the rat to induce the carrageenin footpad edema reaction. In order to examine a degree of swelling of the right hind footpad of the rat, the volume of the right hind leg of the rat was measured with the plethysmometer every one hour from 1 to 5 hours after the induction of the footpad edema reaction, followed by determining the differences between the volume before the induction of the reaction and the volumes after the induction of the reaction. With respect to the rats in the group to which the peptide of the invention was administered, 2 ml/kg of the test liquid containing the peptide of the invention (the peptide of the invention: 0.5 mg/kg) was administered intraperitoneally 24 hours before the induction. With respect to the rats in the group to which aspirin was administered, the test liquid containing aspirin (aspirin: 100 mg/kg) was administered intraperitoneally one hour before the induction. With respect to the rats in the group to which prednisolone was administered, 2 ml/kg of the above-mentioned test liquid containing prednisolone (prednisolone: 5 mg/kg) was administered intraperitoneally one hour before the induction. The same procedure as mentioned above was repeated with the exception of using the above-mentioned aqueous gum arabic solution containing dimethyl sulfoxide and no drugs as a control of the test to examine the degree of swelling of the right hind footpad of the rat. Each test was carried out using five rats. The degree of swelling of the footpad was expressed in the average of the values obtained with respect to the rats. The test results are shown in Fig. 6. As apparent from this figure, the group to which the test liquid containing the peptide of the invention had been administered exhibited a remarkable inhibition of the swelling of the right hind footpad compared with the control group and the effect was higher than that of the group to which aspirin or prednisolone had been administered. Namely, the peptide of the present invention has a higher anallergic inflammation inhibitory activity than aspirin and prednisolone. Test 7 Effect on adjuvant arthritis An effect of the peptide of the invention on rat adjuvant arthritis was examined by the following method. First, the peptide of the invention was suspended in a solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of an aqueous gum arabic solution so that the final concentration of the peptide was 0.25 mg/ml. Prednisolone was suspended in the solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of the aqueous gum arabic solution so that the concentration of prcdnisolonc was 5 mg/ml for a control experiment. The suspensions obtained in this manner were used as test liquids. Male Wistar Lcwis rats having weight of 120 to 200 g were used as test animals. First, 0.1 ml of Mycobacterium tuberculosis H37RA (6 mg/ml) suspended in liquid paraffin was injected subcutaneously into a right hind leg of the rat. Then 2 ml/kg/day of the above-mentioned test liquid (the peptide of the invention: 0.5 mg/kg/day, prednisolone: 10 mg/kg/day) was administered intraperitoneally once a day for 22 days after the injection of mycobacterium. The volumes of right and left hind legs of the rat were measured with a plethysmometer. Differences between the volumes before the injection of mycobacterium and those after the injection of mycobacterium were determined. They are defined as degrees of swelling. The same procedure as mentioned above was repeated with the exception of using the above-mentioned aqueous gum arabic solution containing dimethyl sulfoxide and no drugs instead of the above-mentioned liquid containing the test liquid as a control of the test to examine the degrees of swelling of the right and left hind legs of the rat. Each test was carried out using seven rats. The degrees of swelling were expressed in the averages of the values obtained with respect to the rats. The test results are shown in Fig. 7. As apparent from this figure, the group to which the test liquid containing the peptide of the invention had been administered exhibited remarkable inhibitions of the swellings of the right and left hind footpads compared with the control group and the effect was far higher than that of the group to which prednisolone had been administered. Namely, the peptide of the invention has a higher inhibitory effect on adjuvant arthritis being immune chronic inflammation than prednisolone. Test 8 Granuloma formation The peptide of the invention was dissolved in methanol so that the final concentrations of the peptide were 5 µg/ml, 50 µg/ml, 500 µg/ml and 5000 µg/ml respectively. Into a paper disk having a diameter of 8 mm (filter paper for assay of antibiotics manufactured by Advantech Co., Ltd.) was impregnated 20 µl of each methanol solution having the above-mentioned concentration in two parts and then methanol was removed by air-drying (the peptide of the invention: 0.1 µg/disk to 100 µg/disk). Male Wister rats having weight of 120 to 200 g were used as test animals. The rat was shaved under ether anesthesia and an incision in length of about 3 cm was formed along a back medial line of the rat with a surgical knife. The above-mentioned paper disk was buried subcutaneously through the incision and then the incision was sutured. After one week, the rat was subjected to euthanasia under ether anesthesia. The paper disk was taken out from the subcutaneous site and the wet weight of formed granulation was measured. A paper disk was impregnated with 20 µl of methanol containing no peptide of the invention and air-dried. It was used as a control. The same procedure as mentioned above was repeated with the exception of using a paper disk which had been impregnated with 25 µl of Solcoseryl injection (manufactured by Taiho Pharmaceutical Co., Ltd.) as a reference medicine (Solcoseryl: 1 mg/disk). A paper disk which had been impregnated with 25 µI of physiological saline was used as a control. Each test was carried out using five rats. The wet weight of the granulation was expressed in the average of the values obtained with respect to the rats. The test results are shown in Fig. 8. As apparent from this figure, the groups in which the disk containing the peptide of the invention of not less than 1 µg/disk had been buried exhibited a remarkable increase of the wet weight of granulation compared with the control groups and the reference group of administering Solcoseryl. Accordingly, the peptide of the invention is recognized to have a cell proliferation effect. The effect of the peptide of the invention of 1 µg/disk is recognized to exceed that of Solcoseryl of 1 mg/disk. Test 9 Effect on rat incised wound model The peptide of the invention was dissolved in physiological saline so that the final concentrations of the peptide were 5 µg/ml, 20 µg/ml and 50 µg/ml respectively to prepare test liquids of the peptide of the invention. Male Wister rats having weight of 250 to 300 g were used as test animals. The rat was shaved under ether anesthesia and an incision in length of 3 cm was formed crossing a back medial line of the rat perpendicularly with a surgical knife. The incision was sutured at three points at regular intervals. After 3 hours, 0.5 ml of each test liquid (the peptide of the invention: 2.5 µg/incision, 10 µg/incision and 25 µg/incision) was administered subcutaneously around the incision. For 3 days since the day following the incision, 0.5 ml of each test liquid (the peptide of the invention: 2.5 µg/incision, 10 µg/incision and 25 µg/incision) prepared just before using was administered subcutaneously at the incision once a day. Four days after the incision, the rat was subjected to euthanasia under ether anesthesia. After taking out stitches, a skin piece in strip including 2 cm of the center of the incision was cut off. After a connective tissue of the skin piece was removed, a tensile strength of wound of the incision (g/cm) was measured with a rheometer. As a control, physiological saline was used. As a reference medicine, 0.5 ml of Solcoseryl injection (Solcoseryl: 20 mg/incision, manufactured by Taiho Pharmaceutical Co., Ltd.) was used. Each test was carried out using fifteen rats. The tensile strength of wound (g/cm) was expressed in the average of the values obtained with respect to the rats. The test results are shown in Fig. 9. As apparent from this figure, the groups to which the peptide of the invention had been administered exhibited a remarkable increase of the tensile strength of wound compared with the control group and the reference group of administering Solcoseryl. Namely, the peptide of the invention is recognized to exhibit a higher wound-healing accelerating effect than the Solcoseryl injection. Test 10 Effect on rat skin defect model The peptide of the invention was mixed with an ointment base (Plastibase manufactured by Taisho Pharmaceutical Co., Ltd.) to prepare an ointment containing 0.02% of the peptide of the invention. Male Wister rats having weight of 250 to 300 g were used as test animals. Dexamethasone (1 mg/kg) was administered intraperitoneally to the rat for three days since two days before forming defects. The rat was shaved under ether anesthesia and laid down. The back skin was extended and two skin defects were formed at symmetrical sites with respect to a back medial line of the rat with a punch (16 mm in inner diameter). A sheet of sterilized filter paper (25 × 25 mm) coated with 50 mg of the ointment (containing 10 µg of the peptide of the invention) prepared just before using was applied per a skin defect. The filter paper was covered with Tegaderm (dressings manufactured by Three M Co., Ltd.) and further with adhesive elastic bandage to fix the filter paper. The ointment was administered once a day till healing. The minor axis and the major axis of each defect were measured with a slide gage from the defect-forming day to the healing day and the area of defect was determined regarding the area as an ellipse. The same procedure as mentioned above was repeated with the exception of using the ointment base alone (Plastibase manufactured by Taisho Pharmaceutical Co., Ltd.) as a control. As a reference medicine, 50 mg of Olcenon ointment (tocoretinate: 125 µg/defect, manufactured by Lederle Japan, Ltd.) was used. Each test was carried out using seven rats. The area of defect was expressed in the average of the areas of fourteen defects obtained with respect to the rats. The changes of the defect area with time in the test are shown in Figs. 10 and 11. The complete healing days in the test are shown in Table 15. Complete healing (days, ±S. E.) Control ointment group 17 ±0.7 Ointment containing the peptide of the invention group 14 ±0.4 Olcenon ointment group 18 ±0.4 As apparent from Figs. 10 and 11, and Table 15, the group to which the peptide of the invention had been administered exhibited a significant effect of reduction in the defect area and shortening of the mean of complete healing days compared with the control group. The group to which the peptide of the invention had been administered also exhibited a remarkable reduction effect in the defect area and shortening of the mean of complete healing days compared with the reference group (Olcenon ointment). Thus the peptide of the invention was recognized to exhibit a remarkable wound-healing accelerating effect. Test 11 Toxicity test Toxicity of the peptide of the invention on rats was examined by the following method. First, the peptide of the invention was suspended in a solution obtained by adding dimethyl sulfoxide in an amount of 5% by weight to 5% by weight of aqueous gum arabic solution to prepare a test liquid. Male Wister rats having weight of 120 to 200 g were used as test animals. Then the test liquid was administered subcutaneously to the rats and the state of the rats was observed. As a result, the rats did not die even if 20 mg/kg of the peptide of the invention was administered to each rat. Industrial Applicability The present invention provides a novel peptide represented by the formula [I]. The novel peptide of the present invention exhibits allergic and anallergic inflammation inhibitory, wound-healing effects, and it is useful as an inflammation inhibitor, immunosuppressive agent, wound-healing agent, antiulcer agent, and so forth. Accordingly, the present invention also provides therapeutic agents having the above-mentioned pharmacological effects.

Claims: A novel peptide represented by the formula [I] An anti-inflammatory substance comprising the peptide represented by the formula [I] according to claim 1. An anti-inflammatory preparation comprising the peptide represented by the formula [I] according to claim 1. A wound-healing substance comprising the peptide represented by the formula [I] according to claim 1. An antiulcer preparation comprising the peptide represented by the formula [I] according to claim 1. A wound-healing preparation comprising the peptide represented by the [I] according to claim 1. A process for preparing the peptide according to claim 1, which comprises culturing Actinomyces S. nobilis, extracting an obtained culture medium, a dried product of the medium or a cultured bacterium with an organic solvent, and purifying an obtained extract by column chromatography using a filler comprising silica gel modified with octadecylsilanes. A process for preparing the peptide according to claim 7, wherein an elution solvent of the column chromatography has polarity between a 70% aqueous solution containing a mixed solvent of methanol-acetonitrile (1:1) and methanol-acetonitrile-water (19:19:2). A process for preparing the peptide according to claim 8, wherein at least two elution solvents having the polarity between the 70% aqueous solution containing a mixed solvent of methanonal-acetonitrile (1:1) and methanol-acetonitrile (1:1) and methanol-acetonitrile-water (19:19:2) are made to flow in turn through a column. A process for preparing the peptide according to claim 7, wherein Actinomyces S. nobilis is a preserved bacterium Of the Institute of Physical and Chemical Research (JCM4274), a preserved bacterium ATCC19252 in the United States or a preserved bacterium CBS198.65 in the Netherlands. A process for preparing the peptide according to claim 7 or 10, wherein the extract from the culture medium of S. nobilis, the dried product or the bacterium is treated with ammonium sulfate followed by extracting an obtained precipitate with a solvent.

Inventors: ABE YOSHIKO, FUJIWARA AKIHIKO, INAGAKI KOJI, KURIYAMA KIYOSHI, ABE, YOSHIKO, FUJIWARA, AKIHIKO, INAGAKI, KOJI, KURIYAMA, KIYOSHI, ABE, Yoshiko Sekisui Chemical Co., Ltd., FUJIWARA, Akihiko Sekisui Chemical Co., Ltd., INAGAKI, Koji Sekisui Chemical Co., Ltd., KURIYAMA, Kiyoshi Sekisui Chemical Co., Ltd.
Applicants: SEKISUI CHEMICAL CO LTD, SEKISUI CHEMICAL CO., LTD.
Application year: 1995
Main classification: C07K7
IPC codes: C12P21, A61K38, C07K7