FLUCOSE CONTAINING PROTEOGLYCAN OR ACID GLYCAN AND THEIR PHARMACEUTICAL USE

Abstract: A class of proteoglycans containing fucosylated acidic glycans, e.g., as produced by marine sponges and sea urchin embryos, have been found to stimulate selective proliferation of mammalian natural killer (NK) cells and gamma delta T cells. These compounds are useful as pharmaceuticals, particularly as immunostimulants, e.g., in the treatment of cancer and viral infections.

Description: FUCOSE CONTAINING PROTEOGLYCAN OR AC ID GLYCAN AND THEIR PHARMACEUTICAL USE TECHNICAL FIELD : This invention relates to a class of proteoglycans having fucosylated acidic glyca side chains bound to a protein backbone which have been found to stimulate selectively proliferation of natural killer (NK) cells and/or γδT cells. They are useful as immunostimulants, e.g., in the treatment of cancer and viral infections. PRIOR ART : The proteoglycans of the invention are produced by proliferating cells, for example by sponge cells, sea urchin cells, and, in the case of higher animals (including humans), by embryonic cells and tumor cells. In the natural proteoglycan form, the compounds are large (ca. 5000 to 30,000 kD) extracellular or membrane-bound molecules having a protein backbone which is glycosylated with acidic glycan chains having an unusual polysaccaride sequence containing internal fucose. The structure of the acidic glycan side chains of the proteoglycan isolated from the marine sponge Microciona prolifera has been partially characterized (SpiUmann, et al., J. Biol. Chem (1993) 268: 13378-13387, contents incorporated herein by reference), and we have previously shown that this proteoglycan is involved in cellular aggregation (Misevic, et al., J. Biol. Chem. (1987) 262: 5870-5877; Misevic, et al., J. Biol. Chem. (1990) 265: 20577-20584 ; Misevic, et al., J. Biol. Chem. (1993) 268: 4922-4929, contents of all of these articles incorporated herein by reference). The previously undescribed protein backbone of the Microciona prolifera proteoglycan has now been isolated and characterized, and novel proteoglycans derived from sponges of other genera have also been characterized, as described below. BRIEF DESCRIPTION OF THE INVENTION : It has now surprisingly been discovered that these compounds are potent stimulators of NK cells and γδT cells. In particular, compounds of the invention isolated from an organism of all the phyla and preferably : ♦ from organisms of the Phylum Porifera e.g., of the class Demospongiae, especially of the order Poecilosclerida, family Microcionidae (e.g., of the genus Microciona), or family Mycalidae (e.g., of the genus Mycale), or the order Halichondrida, family Halichondridae (e.g., of the genus Halichondria), or the order Hadromerida, family Clionidae (e.g., of the genus Cliona), or the order Haplosclerida, family Haliclonidae (e.g., of the genus Haliclona), ♦ and/or from organisms of the phylum Echinodermata. These compounds have been shown to stimulate selectively different clones of NK cells and γδT cells. Moreover, it has been found that compounds of the invention have significant anticancer, especially antimetastatic, effects in vivo. It is believed that these anticancer effects are due to stimulation in vivo of NK cells and/or γδT cells. The precise mechanism of this stimulation is unclear, but without intending to be bound by a particular theory, we suggest that these cells may be stimulated by polyvalent interactions with fucosylated acidic glycans of the class described herein and in this way can identify and destroy hyperproliferating cells expressing similar glycan structures. In a pathogenic case, where the hyperproliferating cells are not destroyed in this manner, it is believed that although the hyperproliferating cells produce these acidic glycans, they shed them or present them in monovalent form or other nonstimulatory or inhibitory form, thereby evading detection and destruction by NK cells and/or γδT cells specific for such acidic glycans. Application of the compounds of the invention stimulates NK cells and/or γδT cells specific for such cancer cells, thereby leading to their destruction. Additionally, the compounds of the invention are useful for stimulating NK cells and/or γδT cells against viral or retroviral infections. Finally, in monovalent form, the compounds of the invention are useful for inhibiting the activation of NK cells and/or γδT cells, thereby finding utility as immunosuppressants. The compounds of the invention are selective in their action, in that particular compounds of the invention stimulate only particular clones or subpopulations of NK cells or γδT cells. No significant stimulation of B cells or αβ T cells is observed, so undesirable immunostimulation, e.g., an allergenic or autoimmune response, is avoided. Despite this selectivity, all humans tested, from a variety of ethnic and racial groups, have cell populations capable of being significantly stimulated by the compounds of the invention. Compounds having the glycan structures of the class described herein are found in a wide variety of hyperproliferating cells from sponges to human tumors, thus the basic structure of the compounds is highly conserved. It is hypothesized that compounds of the class described herein act as signals for stimulating the body's defenses against unwanted proliferation of cancerous or infected cells, and that cancers or resistant viral infections may arise when, as described above, these compounds are secreted in nonstimulatory form. Among the examples described herein, it is noted that compounds of the invention isolated from those of the genus Microciona are more effective in stimulating NK cells, as described in example 1 below, whereas compounds isolated from the genus Halichondria are more effective in stimulating γδ T cells, as described in example 9, thus selectivity among cell types receptive to this stimulation is also possible. DETAILED DESCRIPTION OF THE INVENTION The invention thus provides 1. Fucose-containing proteoglycans and acidic glycans, and/or fragment(s) thereof, preferably proteoglycans, e.g., isolated or capable of being isolated from embryonic or neoplastic tissue or from an organism of all the phyla and preferably from an organism of the phylum Porifera, e.g., as described above, especially of the genera Microciona and/or Halichondria and/or Mycale and/or Cliona and/or from an organism of the phylum Echinodermata especially of the genus Lytechinus for use as a pharmaceutical or therapeutic agent in vivo or for ex vivo therapy ; and pharmaceutical compositions comprising such compounds in combination with a pharmaceutically acceptable carrier or diluent. 2. Novel fucose-containing proteoglycans and acidic glycans, and or fragments thereof, preferably proteoglycans, isolated or capable of being isolated from organisms of the genus Halichondria and/ 'or Mycale and/or Cliona. 3. Novel fucose-containing acidic glycans capable of being isolated from a sea urchin of the genus Lytechinus, 4. A Fucose-containing acidic glycan for use as a pharmaceutical or therapeutic agent in vivo or for ex vivo therapy ; and pharmaceutical compositions comprising such compounds in combination with a pharmaceutically acceptable carrier or diluent ; and capable of binding to monoclonal antibodies of the type of these named "Block 2" and described in the reference ".Misevic, et al., J. Biol. Chem. (1993) 268 : 4922-4929, 5. A method of stimulating the proliferation of mammalian, e.g., human, NK cells and/or γδT cells comprising contacting said cells with a compound of the invention (a fucose-containing proteoglycan and acidic glycan, and/or fragment thereof, preferably a proteoglycan and/or fragment(s) thereof, e.g., isolated or capable of being isolated from embryonic or neoplastic tissue or from an organism of the phylum Porifera, or Echinodermata e.g., as described above, especially of the genera Microciona and/or Halichondria and/or Mycale and/or Cliona, and/or of the phylum Echinodermata especially of the genus Lytechinus, in an ex vivo setting or in vivo, e.g., as a vaccine; or a method of treating cancer (e.g., preventing or inhibiting onset, growth, or metastasis of a tumor), or . treating or preventing a viral or retroviral infection, in a mammal, e.g., man; comprising administering a pharmaceutically effective amount of a compound of the invention to a patient in need of such treatment; or the use of a compound of the invention in the manufacture of a medicament for treatment or prevention of cancer or viral or retroviral infections. 6. The use of a fucose-containing proteoglycan or acidic glycan, or fragment thereof, preferably a proteoglycan and/or fragment(s) thereof, e.g., isolated or capable of being isolated from embryonic or neoplastic tissue or from an organism of the phylum Porifera and/or Echinodermata, e.g., as described above, especially of the genera Microciona and/or Halichondria, and/or Mycale and/or Cliona for the phylum Porifera, especially of the genus Lytechinus for the phylum Echinodermata for ex vivo stimulation of proliferation of NK cells and/or γδT cells. 7. A method for screening for or detecting an immunosuppressive (e.g., NK cell and/or γδT cell inhibitory) compound comprising measuring proliferation of NK cells and/or γδT cells in a system containing an NK cell or γδT cell stimulatory concentration of a compound of the invention in the presence and absence of a test compound; and compounds identified using such a method. 8. A gene coding for a protein capable of post-translational glycosylation to form the proteoglycan of the invention, vectors containing such a gene, and transformed cells, especially (i) production cells, e.g., sponge cells, incorporating such a gene for use in producing the desired proteoglycan at enhanced levels or (ii) cancer cells removed from a patient, transformed with the gene so as to express the proteoglycan in stimulatory form, irradiated, and reintroduced into the patient. The gene for Microciona proteoglycan can be isolated, for example, using oligonucleotide probes of a cDNA library based on the disclosed amino acid sequences. Appropriate dosages of the compounds of the invention will of course vary, e.g. depending on the condition to be treated (for example the disease type or the nature of resistance), the effect desired, and the mode of administration. In general however satisfactory results are obtained on administration orally, rectally, nasally, topically, or parenterally, e.g. intravenously, for example by i.v. drip or infusion, at dosages on the order of from 0.01 to 2.5 up to 5 mg kg, e.g. on the order of from 0.05 or 0.1 up to 1.0 mg/kg. Suitable dosages for patients are thus on the order of from 0.5 to 125 up to 250 mg i.v., e.g. on the order of from 2.5 to 50 mg i.v.. Pharmaceutical compositions of the invention may be manufactured in conventional manner, in a suitable aqueous carrier, for example steril buffered physiological saline. For ex vivo stimulation of cells, as described more fully in the example below, suitable amount, e.g., at least 10 ml, of the patient's blood is removed, peripheral bloo mononuclear cells are isolated from the blood, placed in a complete medium in the presenc of a stimulatory concentration of a compound of the invention, e.g., 10-500 μg/ml, ca. 100 μg/ml, optionally in the presence of IL-2, and the culture is maintained until a significan increase in the population of the desired cell type is observed, e.g., ca. 2-4 weeks. Following stimulation of the cells, the cells are isolated from the medium, placed in a injection solution, e.g., sterile buffered physiological saline or plasma, and injected back int the patient. The compound of the invention for this use can, for example, be a proteoglyca or acidic glycan derived from a marine sponge as described in the examples, but may also b a proteoglycan, acidic glycan or fragment thereof isolated from a culture of the cancerous cells to be treated. INDUSTRIAL APPLICATION : The compounds can be useful notably as pharmaceuticals, particularly as immunostimulants, e.g. in the treatment of cancer and viral infections. EXAMPLES : EXAMPLE 1 : Preparation of proteoglycan and acidic glycans from Microciona prolifera a. Extraction of proteoglycan from Microciona prolifera. Fresh marine sponges (Microciona prolifera) collected from the Cape Cod area (USA) are rinsed with 0.5M NaCl, 0.18g/l NaHCO 3 (buffer A) and cut into cubes lxl cm. The cubes are incubated in the buffer A (50% suspension) for 12h at +4°C under gentle rotation. After filtration of the sponge cubes suspension through cheese cloth, the cubes were two more times extracted with the buffer A using the same incubation conditions. The supernatants are either combined or separately centrifuged at 3000 x g for 30 min at +4°C, and the obtained supernatant is again centrifuged at 12,000 x g for 40 min at +4°C. CaCl 2 is added to the supernatant to a concentration of 20mM. After 2-12 h gentle shaking at room temperature, the precipitated proteoglycan is centrifuged at 3000 x g for 20 min at room temperature. The pelleted proteoglycan is dissolved in at lest 20 volumes of 0.5 M NaCl, 2mM CaCl 2 , 20mM Tris pH 7.4 (buffer B) and centrifuged at 10,000 x g for 30 min at +4°C to remove insoluble material. Supernatant was centrifuged at 100,000 x g for 4h at +4°C, and the pelleted proteoglycan redissolved in buffer B at concentration of 1-2 mg/ml To the dissolved proteoglycan in buffer B solid CsCl is added to make a 50% concentration and the solution is centrifuged in a SW rotor at 100,000 x g for 36h at room temperature The pelleted proteoglycan is dialyzed against buffer B and stored at +4°C in the presence o 0.05% NaN 3 . The purified proteoglycan thus obtained exhibits the following characteristics: 1) Molecular mass: 19,000 kD ± 20%. 2) Sedimentation coefficient S 20W : 58 + 20%. 3) Stability to enzymes: Not digestible with Chondroitinase A, B, C, Heparinase, Heparitinase, Hyaluronidase and Keratinase. 4) Gelation: Forms gel in aqueous salt solution containing more then 6mM CaCl 2 or in deionized water. 5) Shape determined with atomic force microscopy in liquid and electron microscopy: circle of 400-500nm diameter with 10-20 arms 200-3 OOnm long. 6) Stability: circle portion dissociates from arms in aqueous salt solutions containing less then lmM CaCl 2 or in the presence of EDTA. 7) Ca 2+ binding determined by flame ionization spectrometry: binds ca. 7000 moles of Ca 2 7mole of proteoglycan at 2mM CaCl 2 and ca. 70,000 moles of Ca 2 7mole o proteoglycan at 20mM CaCl 2 . 8) Dissociation fingerprinting: Dissociation of proteoglycan by 1%SDS at 100°C gave nine fragments ranging from 38 - 1500 kD on a 5-20% linear gradient polyacrylamide gel after electrophoresis. These fragments had apparent molecular masses of ca. 1500 kD, 500 kD, 250 kD, 150 kD, 148 kD, 135 kD, 108 kD, 70 kD, and 38 kD. EDTA and heating at 80°C produced fragments of Mr 1500 x 10 3 , 250 x 10 3 on gel filtration chromatography. Trypsin digestion produced fragments of Mr 124 x 10 3 , 70 x 10 3 , 27 x 10 3 , 10 x 10 3 on gel filtration chromatography. Table I shows approximate amino acid (measured by HPLC pico-tag) and approximate total sugar composition (measured by gas chromatography after methanolysis, reacetylation and silylation) : This proteoglycan consists of approximatively 36 % by weight proteins and 64 % by weight carbohydrates. Standard deviation is less then 20% of each value. Asx signifies Asn or Asp; Glx signifies Glu or Gin. It is also noted that apparant amounts of lie and Leu are somewhat variable depending on the preparation. The amount of uronic acid determined colormetrically is usually 2 times higher then the amount determined by gas chromatography. SO 4 " was determined by HPLC ion chromatography after hydrolysis of PG. The N-terminal sequence of the backbone of the molecule is as follows: Seq. I Pro-Leu-Phe-Thr-Val-Pro-Ue-Tyr-Val-Pro-Glu-Asp-Gln-Leu Seq. π Pro-Glu-Val-Gly-Val-Pro-Ile-Tyr-Val-Pro-Glu-Asp-Gln-Leu Seq. m Pro-Val-Val-Gly-Val-Pro-Ile-Tyr-Val-Pro-Glu-Asp-Gln-Leu preferably Sequence I. Trypsin digestion of the molecule provides peptides having the sequences: Seq. IV Phe-Val-Val-Met-Arg Seq. V Pro-Gln-Asp-Pro-Phe Seq. VI Leu-Ala-Gly-Val-Val-Ile Seq. VQ Pro-Gln-Ala-Ser-Ser-Gly Seq. Vm Ala-Ala-Gln-Trp-Ile-Gly-Gln-Lys b. Isolation of acidic glycans from ihe Microciona prolifera proteoglycan Frozen proteoglycan as obtained above is extracted with water/methanol/chloroform 3/8/4 V/V/V, and the nonlipid fraction was pelleted by centrifugation at 4000 x g for 15 min at +4°C. This extraction is repeated and the pellet is dried under a vacuum. The pellet is wetted in ethanol and resuspended in 0.1 M Tris pH 8, ImM CaCl 2 and 100-200μg Pronase (Calbiochem) (preincubated for 30 min at 60°C in 0.1M Tris pH 8, ImM CaCl 2 per l-2mg dried powder material), and the pellet is digested at 60°C for three days. Two more equivalent portions of preincubated pronase are added at 24 h intervals. DNAse I is then added (30μg) and incubation is continued at 37°C in the presence of lOmM MgCl 2 . The digested sample is then treated again with pronase and chromatographed through G-25 Sephadex (Pharmacia) column eluted with lOmM pyridine acetate pH 5, void volume fractions are collected and lyophilized, and the glycans thus obtained are dissolved in 50mM NaOH in the presence of IM NaHBO 4 and incubated at 45°C for 16h (NaOH treatment may also be omitted). The glycans are passed through Dowex AG 50W-X8 column in H+ form (Bio-Rad) eluted with water, nonbound glycans are immediately neutralized and electrophoresed on a 5-20% or 10-40% linear polyacrylamide gradient gels (Tris/borate- EDTA), and separated acidic glycans of Mr 200 x 10 3 are eluted from gels. (Optionally, the acidic glycans can be sepatated by gel filtration rather than electrophoresis). The isolated acidic glycan molecules are desalted using P-2 column (Bio-Rad) eluted with lOmM pyridine acetate pH 5, lyophilized and stored at -20°C. The acidic glycan fraction is comprised of two major glycans of apparent molecular mass determined by gel electrophoresis using glycosaminoglycan standards of ca. 200 kD and 6 kD. The glycans have the foUowing molar composition (expressed as moles of monosaccharide units / mole of glycan), as determined by gas chromatography, as shown in Table II: Table II 200 kD glvcan 6 kD elvcan Fuc 680 3 Man 20 2 Gal 180 5 GlcNAc 190 14 GlcUA 320 7 Asn 1 1 Standard deviation is less then 20% of each value. Per mole of proteoglycan, there are 20 moles of the 200 kD glycan and 1000 moles of the 6 kD glycan. The glycans are not digestible with Chondroitinase A, B, C, Heparinase, Heparitinase, Hyaluronidase or Keratinase. They are soluble in aqueous solutions and do not form gels in 6mM CaCl 2 salt solutions. At higher concentrations, e.g. > 1 mg/ml water, they will undergo hydrolysis at room temperature. After partial acid hydrolysis of isolated glycans fragments were purified by ion exchange chromatography and high performance liquid chromatography. Methylation analysis, sequential enzymatic and chemical degradation, 1H-NMR spectroscopy, and fast atom bombardment-mass spectrometry of three purified fragments showed following oligosaccharide structures : Structure 1 6 Pyr< >Galβl-4GlcNAcβl-3Fuc 4 is repeated 1000 times per mole proteoglycan. Structure 2 GlcNAcβl-3Fuc 3 SO, is repeated 2000 times per mole proteoglycan. Structure 3 Gala 1 -2Galβ 1 -4GlcNAcβ 1 -3Fuc SO, is repeated 2000 times per mole proteoglycan. EXAMPLE 2 : Preparation of proteoglycans and acidic glycans from Halichondria panicea Extraction of proteoglycan from Halichondria panicea and isolation of acidic glycans from Halichondria panicea proteoglycan is performed as described in example 1 for Microciona prolifera. The proteoglycan thus obtained has the following characteristics: 1) Molecular mass: 10,000 kD ± 20%. 2) Sedimentation coefficient of S 20W 42 ± 20%. 4) Stability to enzymes: Not digestible with Chondroitinase A, B, C, Heparinase, Heparitinase, Hyaluronidase and Keratinase. 5) Gelation: Forms gel in aqueous salt solution containing more then 6mM CaCl 2 or in deionized water. This proteoglycan consists of approximately 79 % protein and 21 % carbohydrate by weight. It has an approximate amino acid composition (as measured by HPLC pico-tag) and approximate total sugar composition (as measured by gas chromatography) as shown in Table UI : Table III Amino acid composition and carbohydrate composition Intact proteoglycan (PG) amino acid mol % Asx 9.1 Glx 9.2 Ser 7.0 Gly 9.9 Arg 7.6 Thr 10.2 Ala 7.0 Pro 8.2 Tyr 4.6 Val 8.6 Met 2.5 Cys 0.1 lie 6.0 Leu 5.5 Phe 4.8 Total 100.0 carbohydrate (mol %) Fuc 12.5 Xyl 1.9 GlcUA 3.2 GalUA 1.7 Man 16.7 Gal 36.2 Glc 13.6 GlcNAc 14.2 Total 100.0 mol/mol PG SO 4 " > 6,250 Standard deviation is less then 20% of each value. Asx signifies Asn or Asp; Glx signifie Glu or Gin. It is also noted that apparant amounts of He and Leu are somewhat variabl depending on the preparation. The amount of uronic acid determined colorimetrically i usually 2 times higher then the amount determined by gas chromatography. SO 4 " was determined by HPLC ion chromatography after hydrolysis of PG. Isolation of acidic glycans from this proteoglycan in the manner described in example 1 gives seven glycans having apparent molecular mass determined by gel electrophoresis using glycosaminoglycan standards of ca. > 1000 kD, 600 kD, 160 kD, 150 kD, 110 kD, 82, kD, and 50 kD. EXAMPLE 3 : Preparation of proteoglycans and acidic glycans from Mycale lingua. Extraction of proteoglycan from Mycale lingua and isolation of acidic glycans from Mycale lingua proteoglycan is performed as described in example 1 for Microciona prolifera. The proteoglycan thus obtained has the following characteristics: 1) Molecular mass: 12,000 kD ± 20%. 2) Sedimentation coefficient of S 20W 48 + 20%. 4) Stability to enzymes: Not digestible with Chondroitinase A, B, C, Heparinase, Heparitinase, Hyaluronidase and Keratinase. 5) Gelation: Forms gel in aqueous salt solution containing more then 6mM CaCl, or in deionized water. This proteoglycan consists of approximately 58% protein and 42% carbohydrate by weight. It has an approximate amino acid composition (as measured by HPLC pico-tag) and approximate total sugar composition (as measured by gas chromatography) as shown in Table IV : Table IV Amino acid composition and carbohydrate composition Intact proteoglycan (PG) amino acid (mol %) Asx 10.8 Glx 9.6 Ser 6.3 Gly 7.7 Arg 9.5 Thr 10.9 Ala 8.0 Pro 7.9 Tyr 0.5 Val 9.0 Met 1.8 Cys 0.2 He 6.2 Leu 6.0 Phe 5.6 Total 100.0 carbohydrate (mol %) Fuc 29.7 Xyl 1.0 GlcUA 11.5 GalUA 0.8 Man 11.0 Gal 15.3 Glc 16.7 GalNAc 6.3 GlcNAc 7.7 Total 100.0 mol/mol PG SO," > 12,000 Standard deviation is less then 20% of each value. Asx signifies Asn or Asp; Glx signifies Glu or Gin. It is also noted that apparant amounts of He and Leu are somewhat variable depending on the preparation. The amount of uronic acid determined colormetrically is usually 2 times higher then the amount determined by gas chromatography. SO 4 " as determined by HPLC ion chromatography after hydrolysis of PG. EXAMPLE 4 : Preparation of proteoglycans and acidic glycans from Ciionacelata : Extraction of two proteoglycans from Cliona celata and isolation of acidic glycans from Cliona celata proteoglycans is performed as described in example 1 for Microciona prolifera with the exception that precipitation with CaCl 2 could be omitted. Two proteoglycan designated CPG1 (more abundant in the first extraction) and CPG2 (more abundant in the second extraction) thus obtained has the following characteristics: 1) Molecular mass: CPG1 >20,000 kD ± 20%; CPG2 6,000 kD. 2) Sedimentation coefficient of CPGl S 20W 125 ± 20%; CPG2 26 S 20W ± 20%. 4) Stability to enzymes: Not digestible with Chondroitinase A, B, C, Heparinase, Heparitinase, Hyaluronidase and Keratinase. 5) Gelation: Both proteglycans form viscous gels in aqueous salt solution containing more then 6mM CaCl 2 or in deionized water. CPGl consists of approximately 26 % protein and 74 % carbohydrate by weight (determined colorimetrically). CPG2 consists of approximately 32 % protein and 68 % carbohydrate by weight. They have an approximate amino acid composition (as measured by HPLC pico-tag) and approximate total sugar composition (as measured by gas chromatography) as shown in Table V : Table V Amino acid composition and carbohydrate composition Intact proteoglycan (CPGl) (CPG2) amino acid (mol %) (mol %) Asx 1.0 7.8 Glx 5.6 9.5 Ser 7.1 11.3 Gly 10.6 10.9 Arg 23.6 6.0 Thr 18.1 14.1 Ala 0.7 7.7 Pro 12.9 10.7 Tyr 8.3 0.7 Val 1.9 6.1 Met 2.4 2.4 Cys 0.3 0.2 He 1.0 3.9 Leu 1.3 5.1 Phe 0.8 3.6 Lys 4.3 0.1 Total carbohydrate (mol %) Fuc 11.0 17.8 Xyl 2.2 2.2 GlcUA 9.4 11.0 GalUA 0.7 1.1 Man 1.2 5.9 Gal 6.8 12.8 Glc 17.2 18.5 GalNAc 32.5 16.2 GlcNAc 19.0 14.8 Total 100.0 mol/mol PG mol mol PG SO 4 " > 20,000 > 6,000 Standard deviation is less then 20% of each value. Asx signifies Asn or Asp; Glx signifie Glu or Gin. It is also noted that apparant amounts of He and Leu are somewhat variabl depending on the preparation. The amount of uronic acid determined colorimetrically i usually 2 times higher then the amount determined by gas chromatography. SO 4 " wa determined by HPLC ion chromatography after hydrolysis of PG. EXAMPLE 5 : Preparation of acidic glycans from Lytechinus pictus : Lytechinus pictus sea urchin eggs and/or embryos (from 2 cell stage to plutes stage) were washed with sterile sea water and pelleted embryos were extracted with water/methanol/chloroform 3/8/4 V/V/V, and the nonlipid fraction was pelleted by centrifugation at 4000 x g for 15 min at +4°C. This extraction is repeated and the pellet is dried under a vacuum. The pellet is wetted in ethanol and resuspended in 0.1 M Tris pH 8, ImM CaCl 2 and 100-200μg Pronase (Calbiochem) (preincubated for 30 min at 60°C in 0.1M Tris pH 8, ImM CaCl,per l-2mg dried powder material), and the pellet is digested at 60°C for three days. Two more equivalent portions of preincubated pronase are added at 24 h intervals. DNAse I is then added (30μg) and incubation is continued at 37°C in the presence of lOmM MgCl 2 . The digested sample is then treated again with pronase and chromatographed through G-25 Sephadex (Pharmacia) column eluted with lOmM pyridine acetate pH 5, void volume fractions are collected and lyophilized, and the glycans thus obtained are dissolved in 50mM NaOH in the presence of IM NaHBO 4 and incubated at 45°C for 16h (NaOH treatment may also be omitted). The glycans are passed through Dowex AG 50W-X8 column in H+ form (Bio-Rad) eluted with water, nonbound glycans are immediately neutralized and electrophoresed on a 5-20% or 10-40% linear polyacrylamide gradient gels (Tris/borate-EDTA), and separated acidic glycans of Mr 200 x 10 3 are eluted from gels. (Optionally, the acidic glycans can be sepatated by gel filtration rather than electrophoresis). The isolated acidic glycan molecules are desalted using P-2 column (Bio-Rad) eluted with lOmM pyridine acetate pH 5, lyophilized and purified by affinity chromatography with the Block 2 monoclonal antibodies of ref Misevic et al mentioned above stored at -20°C. 1) Molecular mass: 580 kD ± 20%. 2) Sedimentation- coefficient 8.5 S 20W ± 20%. 4) Stability to enzymes: Not digestible with Chondroitinase A, B, C, Heparinase, Heparitinase, Hyaluronidase and Keratinase. 5) Gelation: self-interacton-oligomerization in aqueous salt solution containing more then 6mM CaCl 2 or in deionized water. Table VI mol carbohydrate mol acidic glycan (mol . Fuc 737 25.40 Xyl 108 3.73 Gal 39 1.34 Glc 12 0.41 Uronic acids 786 27.10 GalNAc 506 17.46 GlcNAc 712 24.56 Total 2,900 100.00 mol/mol SO 4 " 1.600 Standard deviation is less then 20% of each value. The amount of uronic acid determined colormetrically is usually 2 times higher then the amount determined by gas chromatography. SO " was determined by HPLC ion chromatography after hydrolysis of PG. EXAMPLE 6 : Ex vivo stimulation of human NK cells proliferation by Microciona prolifera proteoglycan and by its acidic glycans Human peripheral blood mononuclear cell (PBMC) are isolated from 10 ml of blood by centrifugation on Ficoll gradient (Pharmacia). Stimulation of PBMC proliferation with 100 μg/ml acidic glycans or proteoglycans is performed in the presence of complete medium (RPMI 1640, 5% human AB serum, 2mM L-Glutamine, ImM Na pyruvate, non-essential amino acids and 50μg/ml Kanamycin). After 5 days 5U/ml of human recombinant JL-2 is added . One half of medium is changed when it becomes acidic. After 7, 14, 21, 28 and 35 days cells were analyzed by FACS using antibodies against following markers: CD3, TCR αβ, TCR γδ, CD4, CD8 - T cells; CD16, CD56 - NK cells; CD20 - B cell; CD14 - monocytes. Results from five different donors after 3 weeks: In the PBMC cultures treated with acidic glycans, NK cells population (CD 16 and CD 56 positive) and (CD3, TCR αβ, TCR γδ, CD4, CD8, CD20 and CD14 negative) increased from 1-5 % to 30-80 % of the total PBMC, whereas untreated controls remained at a level of 1-5 % NK cells. Specific stimulation of NK cells proliferation by glycans was confirmed by 3 H thymidine incorporation only in isolated clones of NK cells and not αβT cells isolated from the same PBMC cultures. EXAMPLE 7 : Ex vivo stimulation of human NK cells proliferation by Mycale lingua an Cliona celata proteoglycans and by its acidic glycans was similar to Microciona prolifera proteoglycan. EXAMPLE 8 : Ex vivo stimulation of human NK cells proliferation by Lytechinus pictu acidic glycan with 580 kD was similar to Microciona prolifera proteoglycan. EXAMPLE 9 : Stimulation of human γδT cells proliferation (ex vivo) by Microcion prolifera proteoglycan, Halichondria panicea proteoglycan and /or their acidic glycans Same culturing procedure as described in the previous example shows that Microciona prolifera acidic glycans stimulate only one subpopulation of γδT cells via T cell receptor with an increase from 5% to 20%. Halichondria panicea proteoglycan and its acidic glycans stimulate a different population of γδT cells from 5% to 70%. These data are confirmed by 3 H thymidine incorporation in isolated clones stimulated by specific acidic glycans. EXAMPLE 10 : Anti-tumorogenic and anti-metastatic activity of proteoglycans from Microciona prolifera (in vivo) Seven C-57 black mice are injected i.p. with 300μg proteoglycan from Microciona prolifera/200μ\ 0.2M NaCl, 2mM CaCU, 20mM Tris pH 7.4/animal, every day for five days. At day five, animals are injected with 2.5 X 10 4 B-16 melanoma cells per animal. Animals are immunized for five more days with proteoglycan as described above. The appearance of tumor, tumor growth, survival of animals and appearance of metastasis are observed in immunized animals and compared with control animals injected with buffer. Control animals which have not received proteoglycan all exhibit marked melanoma growth followed by metastasis. Compared to controls, treated animals exhibit a 20% delay in the time of appearance and 50% reduction in growth of syngenic B16 melanomas, a 12% increase in the total time of survival of all immunized mice (p = 0.0044), and complete inhibition of metastasis. EXAMPLE 11 : Anti-tumorogenic and anti-metastatic activity of proteoglycans and their acidic glycans from Halichondria panicea Mycale lingua, Cliona celata and Lytechinus pictus were similar to to Microciona prolifera proteoglycan (in vivo). EXAMPLE 12 : Cloning and expression of gene for proteoglycans from Microciona prolifera Proteoglycan (PG) cDNA is isolated from a random-primed cDNA library created using poly(A) + RNA from Microciona prolifera cells. This cDNA library is screened using the N-terminal amino acid sequence of PG described in example 1 above by colony hybridization techniques, i.e., expressing the library in an expression system, preferably E. coli, lysing the colonies, e.g., on nitrocellulose filters, denaturing their DNA in situ and fixing it on the filter, hybridizing with labeled, preferably radiolabeled, oligonucleotide probes of at least 30 base pairs having cDNA base sequences corresponding to all or a portion of the N-terminal sequence of PG, identifying hybridized colonies, and retrieving the corresponding vectors from the library, using chromosome walking techniques if necessary to isolate and characterize one or more cDNA fragments containing one or more regions coding for glycosylation sites for N-linked glycans. (Note that the cDNA is repetitive, so it is not necessary to clone, isolate and characterize the entire sequence). Once the desired portion of cDNA has been isolated, it is expressed in a suitable expression system, preferably a eukaryotic system, most preferably a sponge. The PG is isolated from the sponge or from the culture medium of the expression system, e.g., using the procedures outlined above.

Claims: CLAIMS 1. A fucose-containing proteoglycan or acidic glycan and or fragment(s) thereof, for use as pharmaceutical. 2. A fucose-containing proteoglycan or acidic glycan according to claim 1 capable of being isolated from an organism of all phyla and preferably of the phylum Porifera and/or of the phylum Echinodermata. 3. A fucose-containing proteoglycan or acidic glycan according to claim 2 capable of being isolated from a marine sponge of the genus Microciona and/or Halichondria and/ 'or Mycale and/or Cliona. 4. A fucose-containing proteoglycan or acidic glycan capable of being isolated from a marine sponge of the genus Halichondria and/or Mycale and/or Cliona. 5. A fucose-containing acidic glycan capable of being isolated from a sea urchin of the genus Lytechinus. 6. A fucose-containing acidic glycan according to claim 1,2 or 5, capable of binding to monoclonal antibodies of the type of those named "Block 2" and described in the reference "Misevic, et al., J. Biol. Chem. (1993) 268 : 4922-4929. 7. A method of stimulating the proliferation of mammalian NK cells and/or γδT cells of larger mammals, e.g., man, comprising contacting said cells with a compound as described in any one of claims 1-6. 8. Use of a compound according to any one of claims 1-6 in " the manufacture of a medicament for treating cancer or viral or retroviral infections. 9. A pharmaceutical composition comprising a compound according to any one of claims 1-6, together with a pharmaceutically acceptable diluent or carrier. 10. A kit of parts for ex vivo stimulation of proliferation of mammalian NK cells and/or γδT cells comprising a compound as described in any one of claims 1-6. 11. A method for screening for or detecting an immunosuppressive compound comprising measuring proliferation of NK cells and/or γδT cells in a system containing an NK cell or γδT cell stimulatory concentration of a compound as described in any one of claims 1-6 in the presence and absence of a test compound. 12. All novel compounds, processes and utilities substantially as described herein.

Inventors: MISEVIC GRADIMIR, MISEVIC, GRADIMIR
Applicants: MISEVIC GRADIMIR, MISEVIC, GRADIMIR
Application year: 1995
Main classification: C07K14
IPC codes: A61K38, C07K14, C08B37