AMIDES OF MONO AND BICARBOXYLIC ACIDS WITH AMINO ACIDS OR GLYCOSAMINES, SELECTIVELY ACTIVE ON THE CANNABINOID PERIPHERAL RECEPTOR

Abstract: The vast number of theoretically conceivable compounds resulting from the combination of all claimed substituents in formula (I) precludes a comprehensive search. For economical reasons the search has been limited to the following compounds: R1 is a hydrocarbon radical unsubstituted or substituted by only one OH-group and having a linear chain of at least 8 carbons atoms(A); the NR2R3 group is either a linear alpha-, beta-, or gamma-, amino acid (e.g. amino acids like proline are not included), or a glycosamine residue. Despite the above limitation the search revealed too many relevant compounds. Therefore the search was further limited to compounds with NR2R3 = Glycine, beta-Alamine, gamma-amino-butyric acid, Serine- or Cysteine derivatives, or glycosamine of the following structures: (a), (b), (c). Still too many pertinent compounds where retrieved which can not all be cited in the search report. The documents cited are only an arbitrary selection and the search report has to be regarded as incomplete.

Description: AMIDES OF MONO AND BICARBOXYLIC ACIDS WITH AMINO ACIDS OR GLYCOSAMINES, SELECTIVELY ACTIVE ON THE CANNABINOID PERIPHERAL RECEPTOR FIELD OF THE INVENTION The present invention refers to new amides of mono and bicarboxylic acids with aminoalcohols, therapeutically active in the treatment of pathologies connected with the modulation of the peripheral receptor for cannabinoids. STATE OF THE ART Cannabinoids are a specific class of psycho-active compounds present in hemp (Cannabis sativa) , comprising more than 60 different molecules, the most important of which are: cannabinol, cannabidiol and many isomers of tetrahydrocannabinol. The therapeutic activity of Cannabis is known since the old Chinese dinasties which used it already 5000 years ago in the treatment of asthma, migraine and some gynecological disorders. The use was confirmed along the years and in 1850 the Cannabis extracts where entered the American Pharmacopheia and maintained up to 19^7• Cannabinoids are responsible of many effect on different systems and/or organs; the most relevant effects are on the central nervous system and on the cardiovascular system. In fact, they affect mood, memory, motory coordination and cognition, increase heart rate and modify the systemic arterious pressure. It is also known that cannabinoids are capable of lowering the intraocular pressure and produce effects on the respiratory and endocrin system (L.E. Hollister, Health Aspects of Cannabis, Pharmacological Reviews, 38, 1- 20. 1986). More recently also their capacity of suppressing the cellular and humoral immune response and their antiinflammatory properties were described (A. . irth et al . , Antiinflammatory properties of Cannabichromene, Life Science 26, 1991-95. 1980). However, the use of Cannabis in therapy is debated, because of its relevant psychoactive effects, which gives addiction and dependence, and its various side effects which up to now are not completely known (L.E. Hollister, 1986, cited above). In spite of the large use made along the centuries, the mechanism of action of cannabinoids was for long unknown. Only in 1990 Matsuda et al. could identify and clone a receptor for cannabinoids which was discovered to be a member of the super-family of the G-protein-coupled receptors; CBl is coupled to Gl to inhibit adenialte cyclase activity and to a pertussis-sensitive G protein to regulate Ca currents. Such receptor is mainly localised in the brain, in cell-lines of neurvous origin and only to a limited extent at peripheral level; therefore, depending on its location, it was named Central Receptor (CBl) (Matsuda et al.. Structure of a cannabinoid receptor and functional expression of the cloned cDNA, Nature 346: 61- 64, 1990). The discovery of a receptor led to the assumption that the esistence of a specific endogenous ligand. Other studies permitted to isolate in the pig brain a subtance capable of producing an antagonist effect, i.e. capable of competitively binding to the cannabinoid central receptor. The structural study and the comparative tests with the synthetic product permitted to identify such subtance, which resulted to be an amidic derivative of arachidonic acid, and more particularly the arachidonylethanolamide, later named anandamide. The pharmacological characterization of such molecule showed that anandamide possesses an activity profile similar, although less potent, than 9-THC (tetrahydrocannabinol with a double bond in position 9). and is able to mimic its psychoactive effects. Such results brought to the conclusion that anandamide is the endogenous ligand of the central receptor for cannabinoids (C. C. Felder et al . Anandamide, an endogenous cannabimimetic eicosanoid, binds to the cloned human cannabinoid receptor and stimulates receptor-mediated signal transduction, PNAS. 90, 7656-7660,1993; P.B. Smith et al . The pharmacological Activity of Anandamide, a Putative Endogenous Cannabinoid. in Mice, J. PET. 270, 219-227. 1994). Following studies permitted to identify substances capable of binding to the receptor CBl, which were grouped in a class of amidic compounds named anandamides by the authors (Hanus L. et al. Two New Unsatured Fatty Acid Ethanola ides in Brain That Bind to the Cannabinoid Receptor, J. Med. Chem., 36, 3032-3034, 1993). The discovery that the ethanolamide of arachidonic acid, but not the ethanolamide of other acids which are biologically important and anyway endogenously present at the cerebral level (such as palmitic acid) , is capable of functionally activating CBl central receptor, brought about the subsequent identification of other amides of ethanolamine with highly unsaturated fatty acids which have an affinity to CBl receptor. Its peculiar distribution led to assume the existence of differentiated receptor sites. In fact, a second different receptor for cannabinoids was cloned, named Peripheral Receptors (CX5 or CB2) . Since such receptor was identified in the spleen and macrophages/monocytes, being on the other hand absent at the central level, it is considered that this receptor mediates the non psycho¬ active effects of the cannabinoids (S. Munro et al . , Molecular characterisation of a peripheral receptor for cannabinoids, Nature, 365. 61-65, 1993)- In this connection there are some evidences of the capacity of^9-THC to induce immunosuppressive effects. Recent experimental studies demonstrated that Δ.9-THC is capable of influencing the function of macrophage. Exposition to \ Q-THC lowers the cytolytic action of activated macrophages, measured as synthesis, release and citotoxicity of TNF-α. Anyhow, since the macrophages release various molecules having a cytolytic potential, other than TNF-α, it is considered that they can represent a target for£9-THC (K. Fischer-Stenger et al. ,^9 _ tetrahydrocannabinol Inhibition of Tumor necrosis Factor-alpha: Suppression of Post-translational Events, J. PET. 267, 1558-1565, 1993). All these evidences and the preferential massive localisation of CB2 receptor in the immuno system confirm that such receptor plays a specific role in mediating the immune and antiinflammatory response to stimuli of different nature, included the bacterial and the viral ones. It was also demonstrated that anandamide, endogenous ligand for the CBl central receptor, is capable of binding to the CB2 receptor with an affinity which is 30 folds inferior to that of the central receptor; this probably implies the existence of a different endogenous ligand for such receptor, up to now still unknown (L. L. Iversen, Medical uses of marijuana?. Nature, 5. 12-13, 1993)- As already mentioned, the therapeutical use of cannabinoids as analgesics, antiemetics, anticonvulsivants, antispastics, antiglaucoma and, more recently, antiinflammatory, is limited by the presence of undesired side effects, or psychoactive effects, and by the outcome of addiction and pharmacological tolerance (W.L. Dewey , Cannabinoid Pharmacology, Pharmacological Reviews, 38, 151-178, 1986). Recently some compounds were prepared, capable of acting as agonists on both the cannabinoids receptors: for example it is known the use of derivatives of dihydropyrrole-(l,2, 3 _ d,e)-1 ,4-benzoxazine in the treatment of glaucoma (US 5 112 820, Derwent abstract) and it is also known the use of derivatives of 1 ,5-diphenyl-pyrazole as immunomodulators or psychotropic agents in the treatment of various neuropathologies, migraine, epilepsy, glaucoma ecc. (European Patent Application EP 576 357. Derwent abstract). However, such compounds, being active on both CBl central and CB2 peripheral receptor, can lead to the outcome of serious psychoactive effects, a part from addiction and dependence. In the light of the fact that the cannabinoids act through a receptorial mechanism and especially on receptors capable of mediating different functional effects, and also in view of the low homology between the Peripheral and Central Receptors, it is evident the importance of developing a class of drugs selectively active on the receptor sub-type and not indiscriminately on both the receptors such as the natural or synthetic cannabinoids. The search on the pharmacological effects mediated by cannabinoids receptors shows that the non-psychoactive effects of Cannabis derivatives are mediated by CB2 peripheral receptor. Furthermore, receptor CB2 localisation confirms that such non-psychoactive effects, i.e. the effects on the immune system and the antiinflammatory, myorelaxant and antinociceptive effects, as well as the influence on the pressure systems, are mediated by such receptor. It is therefore extremely important to obtain compounds which are capable of selectively acting on the peripheral receptor for cannabinoids and. therefore, on the pathologies connected with the modulation of such receptor, excluding the psychoactive effects at central level and the relevant side effects related to such action, such as addiction and dependence. SUMMARY OF THE INVENTION The Applicant has now found a new class of amidic derivatives of aliphatic mono and bicarboxylic acids with aminoalcohols, able to bind selectively to the cannabinoid peripheral receptor CB2 and to activate functionally the same. The new amidic derivatives according to the present invention have formula (I) : 3 wherein Ri is: 1) a linear or branched hydrocarbon radical having from 9 to 23 carbon atoms, preferably from 11 to 17. saturated or presentig one double bond, optionally substituted with one or more -OH groups; 2) a group of formula (II): 0 R II / 5 - R 4 - C - N (II) R 6 wherein R/, is a linear hydrocarbon radical, saturated or containing one double bond, comprising from 8 to 22 carbon atoms, preferably from 10 to 16, optionally substituted with one or more linear or branched alkyl groups ^-Cg and/or with one or more -OH groups; 3) a group of formula (IV): 0 II - R 4 - C - 0 - Rγ (IV) wherein R^ has the meanings decribed above and Ry is -H or a linear or branched alkyl group comprising from 1 to 20 carbon atoms. In formula (I) the residue of formula (III): - N (III) \ R 3 can represent the aminic residue of an aminoacid (A) or of a glycosamine (B) , and in particular: A) the residue of formula (III) is the residue of an optically active or non active aminoacid, wherein R 2 , together with the nitrogen atom to which it is bound, forms an α-aminoacid, optionally presenting an aliphatic or arylaliphatic lateral chain optionally substituted with -OH, -OP0 3 H 2 , -0-P0 2 H-0-CH 2 -CH(0H)-CH 2 -0H, -SH or S-CH-^; or it forms, together with the nitrogen atom to which it is bound, a β- or V-aminoacid presenting an aliphatic or arylaliphatic lateral chain; the acid group of such aminoacid being optionally methylated or ethylated; R^ is -H or -CH,; when R 1 belongs to class (1) and R 2 forms, together with the N atom to which it is bound, the aminoacylic residue of alanine, (J-alanine or glycine, R-, is different from -CH ; B) in the residue of formula (III), R 2 forms, with the nitrogen atom to which it is bound, the residue of a glycosamine having formula wherein one of the groups P, Y and Z is -N-CO-Ri and the I R 3 other groups can be -H or -OH, R-, being as above defined and Rn being In formula (II) the residue of formula (V): -N Λ (V) \ R 6 can be the same as (III), defined above, with Rr and Rg equal or different from R 2 and R . The residues of formulae (III) and (V), belonging both to classes (A) and (B) , can be optionally homogeneously substituted on R , R , Re and/or R^ with at least one pharmaceutically acceptable radical capable of increasing the solubility of the compounds of the present invention in water, alcohols and suitable organic solvents. Said pharmaceutically acceptable radical is preferably selected from the group consisting of acyls (i.e. acetyl and benzoyl), emiacyls of dicarboxylic acids (i.e. succinic and glutaric acid), amino-acyles, dialkylamino-acyls, alkyl-sulphonates (i.e. metansulphonate and p- toluen-sulphonate) , alkyls (i.e. methyl and ethyl), 0-phosphates and 0-sulphates . A further object of the present invention is the use of the described derivatives in the treatment of pathologies of mammals connected with the modulation of the cannabinoid peripheral receptor. Other objects of the present invention are the process for the preparation of the above described new compounds and the pharmaceutical compositions containing therapeutically active quantities of such amidic derivatives and of their more soluble and/or slow release form, for the therapeutic treatment of pathologies connected with the modulation of the cannabinoid peripheral receptor. DESCRIPTION OF THE DRAWINGS Figure 1 illustrates the specific hybridization of cannabinoid peripheral receptor amplified by Polymerase Chain Reaction (PCR) in A) rat spleen, B) rat peritoneal mast cell cultures and C) RBL-2H3 cell cultures. Figure 2 illustrates the specific mRNA-CB2 cannabinoid peripheral receptor detected by in situ hybridisation in Cerebellar granule cells (Fig. 2A) and Cerebellum (Fig, 2B). DETAILED DESCRIPTION OF THE INVENTION The advantages and characteristics of the new class of amides of mono or bicarboxylic acids with aminoalcohols, selectively active on the cannabinoid peripheral receptor, the process for their preparation, their pharmaceutical use and the pharmaceutical compositions containing them, according to the present invention, will be better illustrated in view of the following detailed description. The amides of mono and bicarboxylic acids with aminoalcohols according to the present invention have formula (I): 0 R-, R : - C :ι; - 9a - When R-^ belongs to class (1), it forms, with the adjacent carbonyl. the acyl group of a monocarboxylic acid, preferably lauric. myristic. palmitic, stearic. palmitoleic, oleic acid and their homologues substituted with hydroxyl groups, as for example -hydroxypalmitic acid. When R belongs to classes (2) and (3). R4 forms, together with the two adjacent carbonyls, the acyl group of a bicarboxylic acid, preferably traumatic acid. - 10 As regards the aminic moiety of formula (I) , when the residue of formula (III) belongs to class (A), such aminoacid is preferably glycine, β-alanine, y -amino-butyric acid, serine and cysteine. Are known in the state of the art the compounds of formula (1) wherein R- j^ belongs to class (1) and R forms, together with the nitrogen atom to which it is bound, the aminoacylic residue of alanine, β-alanine and glycine, and R-, = CHT. When the residue of formula (III) belongs to class (B), such glycosamine is preferably D-Glucosamine, L-Acosamine, D-Mannosamine, D-Galactosamine. The process for the preparation of said amides of formula (I) , according to the invention, involves the reaction of an activated form of the acid R j^ -COOH, of formula R-^CO-X, with an amine of formula H - N \ R 3 in an aqueous basic medium or in organic solvents in the presence of bases, under stirring at a temperature ranging from -20° to 6θ°C, and preferably from -10 * to 20"C, for a period ranging from 10 minutes to 24 hours, and preferably from 1 to 3 hours. After the optional acidification of the reaction medium, the desired product is isolated and purified by conventional techniques. Said activated form of the acid can be an acyl halide, a succinimide- ester, an acylisourea, a p-N0 2 -phenylester or a methylester; said base is preferably K 2 C0 , K0H, Et-^N or N-methyl-morpholine. The following examples of preparation of the amides of mono and bicarboxylic acids according to the present invention are reported for - 11 - illustrative but not limitative purposes. EXAMPLE 1: Preparation of N-palmitoyl-L-serine 1.58 g of L-serine (15 mmol) are solubilized at 4°C in 60 ml of K 2 C0 IM. The solution is slowly added, dropwise under stirring, over a period of 30 minutes, with 2.75 g of palmitoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, thereafter is acidified with hydrochloric acid 6N. The precipitated crude product is separated by filtration, dried under vacuum and crystallized a first time from 30 ml of ter-butyl-methyl-ether and thereafter from 30 ml of cold methanol. The crystallized product is separated by filtration, washed twice with 5 ml methanol and dried under high vacuum. The reaction reaction yield is about 89%. The physico-chemical properties of N-palmitoyl-L-serine are as follows: -physical state: white crystalline powder -molecular formula: C^nH yN0i -molecular weight: 343-5 -elemental analysis: C= 66.43 . H= 10.86 N= 4.08% 0= 18.63% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble -melting point: 97-99°C -TLC: eluent: chloroform/methanol/water/ NH 3 (28%) 80:25:2:1 Rf = 0.15 EXAMPLE 2: Preparation of N-lauroyl-L-serine 1.58 g of L-senne (15 mmol) are solubilized at 4°C in 40 ml of K0H - 12 IN. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.19 g of lauroyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, thereafter is acidified with hydrochloric acid 6N and extracted three times with 20 ml ethyle acetate; the organic phases are collected, dried with anhydrous sodium sulphate and evaporated under vacuum. The crude product is crystallized from 30 ml of cold acetonitrile. The crystallized product is separated by filtration, washed twice with 5 ml acetonitrile and dried under high vacuum. The reaction yield is about 85%. The physico-chemical properties of N-lauroyl-L-serine are as follows: -physical state: white crystalline powder -molecular formula: C j cH^ O/ j -molecular weight: 287-40 -elemental analysis: O 62.69% H= 10.17% N- 4.87% 0= 22.27% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble (> 10 mg/ml as sodium salt) -melting point: 120-122 * C -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 Rf * 0.46 EXAMPLE 3: Preparation of N-oleoyl-L-serine 1.58 g of L-serine (15 mmol) are solubilized at 4°C in 60 ml of K 2 C0 3 IM. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 3.01 g of oleoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0 β C overnight. - 13 - thereafter is acidified with hydrochloric acid 6N. The precipitated crude product is separated by filtration, dried under vacuum and purified by silica-gel column chromatography using as eluent a mixture of chloroform/methanol/water/acetic acid 90:10:0.5:0.1. The fractions of eluate containing the product are collected, evaporated to dryness and the residue is dried under high vacuum. The reaction yield is about 88%. The physico-chemical properties of N-oleoyl-L-serine are as follows: -physical state: white amorphous powder -molecular formula: -molecular weight: 369-5^ -elemental analysis: C= 68.26% H= 10.64% N= 3.79% 0= 17-32% -solubility in org. sol.: >10 mg/ml in DMSO -water solubility: slightly soluble -melting point: -TLC: eluent: chloroform/methanol/water/ NH^ (28%) 80:25:2:1 Rf = 0.14 EXAMPLE 4: Preparation of N-palmitoleyl-L-serine I.58 g of L-serine (15 mmol) are solubilized at 4°C in 60 ml of I^CO^ IM. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.73 g of palmitoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, thereafter is acidified with hydrochloric acid 6N and extracted three times with 20 ml ethyle acetate; the organic phases are washed twice with 15 ml of water, collected and evaporated under vacuum. The crude product is purified by preparative silica-gel column chromatography, using as eluent a mixture of chloroform/methanol/water/acetic acid - 14 - 90:10:0.5:0.1. The eluate fractions containing the product are collected, evaporated to dryness and the residue is dried under high vacuum . The reaction yield is about 87%. The physico-chemical properties of N-palmitoleyl-L-serine are as follows: -physical state: white amorphous powder -molecular formula: -molecular weight: 3 1.49 -elemental analysis: C« 66.83% H= 10.33% N « 4.10% 0= 18.74% -solubility in org. sol.: >10 mg/ml in DMSO -water solubility: slightly soluble -melting point: -TLC: eluent: toluene/ethanol/acetic acid 65=30:5 Rf: 0.45 EXAMPLE 5: Preparation of N-lauroyl-L-serine methylester 2.33 g of L-serine methylester hydrochloride (15 mmol) are solubilized at 4°C in 40 ml of a mixture tetrahydrofurane/water 9:1 with 4.05 g of triethylamine (40 mmol). The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.19 g of lauroyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0 * C for 2 h and at room temperature for a night and evaporated to dryness under vacuum. The crude product is suspended in 50 ml of hydrochloric acid IN. extracted three times with 20 ml ethyle acetate; the organic phases are washed twice with 15 ml water, collected, dried on anhydrous sodium sulphate and evaporated under vacuum. The crude product is crystallized from 30 ml of cold hexane. The crystallized - 15 - product is separated by filtration, washed twice with 5 ml hexane and dried under high vacuum. The reaction yield is about 87%- The physico-chemical properties of N-lauroyl-L-serine methylester are as follows: -physical state: white crystalline powder -molecular formula: -molecular weight: 301.43 -elemental analysis: C= 63-75% H= 10.37% N= 4.65% 0= 21.23% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble -melting point: 63-65°C -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 Rf= 0.60 EXAMPLE 6: Preparation of N-palmitoyl-L-cysteine 1.82g of L-cysteine (15 mmol) are solubilized at 4°C in 60 ml of K 2 C0 IM. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2-75 g of palmitoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, therefater is acidified with hydrochloric acid 6N. The crude precipitate is separated by filtration, dried under vacuum and crystallized a first time from 30 ml of ter-butyl-methyl-ether and thereafter from 30 ml of cold methanol. The crystallized product is separated by filtration, washed twice with ml methanole and dried under high vacuum. The reaction yield is about 92%. - 16 - The physico-chemical properties of N-palmitoyl-L-cysteine are as follows: -physical state: white crystalline powder -molecular formula: C- j nH-jyNO S -molecular weight: 359-57 -elemental analysis: C» 63.47%, H= 10.37%. N= 3-90%, 0= 13-35%. s « 8.92% -solubility in org. sol.: >10 mg/ml in DMSO -water solubility: slightly soluble -melting point: -TLC: eluent: chloroform/methanol/water/ NH 3 (28%) 80:25:2:1 Rf: 0.42 EXAMPLE 7: Preparation of N-palmitoyl-glycine 1.13 g of glycine (15 mmol) are solubilized at 4"C in 40 ml of K0H IN. The solution is slowly added, dropwise under stirring over a p riod of 30 minutes, with 2.75 g of palmitoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, therefater is acidified with hydrochloric acid 6N. The crude precipitate is separated by filtration, dried under vacuum and crystallized from 60 ml of cold ethanol 95". The crystallized product is separated by filtration, washed twice with 5 -1 ethanol 95" and dried under high vacuum. The reaction yield is about 79%. The physico-chemical properties of N-palmitoyl-glycine are as follows: -physical state: white crystalline powder -molecular formula: -molecular weight: 313.^δ - 17 - -elemental analysis: C= 68.97% H= 11.25% N= 4.47% 0= 15-31% -solubility in org. sol.: >10 mg/ml in DMSO -water solubility: slightly soluble -melting point: 121-123'C -TLC: eluent: chloroform/methanol/water NH 3 (28%) 80:25:2:1 Rf: 0.22 EXAMPLE 8: Preparation of N-lauroyl-glycine 1.13 g of glycine (15 mmol) are solubilized at 4°C in 40 ml of K0H IN. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.19 g of lauroyl-chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, therefater is acidified with hydrochloridic acid 6N and extrated three times with 20 ml ethyle acetate; the organic phases are collected, dried with anhydrous sodium sulphate and evaporated under vacuum. The crude product is crystallized from 30 ml of cold acetonitrile. The crystallized product is separated by filtration, washed twice with 5 ml acetonitrile and dried under high vacuum. The reaction yield is about 83%. The physico-chemical properties of N-lauroyl-glycine are as follows: -physical state: white crystalline powder -molecular formula: -molecular weight: 257-37 -elemental analysis: C= 65-34% H= 10.57% N= 5-44% 0= 18.65% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble (>10 mg/ml in phosphate buffer 50 mM, pH 7-4, NaCl 0.9%) - 18 - -melting point: 117-119 * 0 -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 Rf = 0.56 EXAMPLE 9: Preparation of N-lauroyl-glycine methylester 1.88 g of glycine methyl ester hydrichloride (15 mmol) are solubilized at 4°C in 40 ml of a mixture tetrahydrofurane/water 9:1 with 4.05 g of triethylamine (40 mmol) . The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.19 g of lauroyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C for 2 h and at room temperature for a night and evaporated to dryness under vacuum. The crude product is suspended in 50 ml of hydrochloric acid IN, extracted three times with 20 ml ethyle acetate; the organic phases are washed twice with 15 ml water, collected, dried on anhydrous sodium sulphate and evaporated under vacuum. The crude is crystallized from 30 ml of cold hexane. The crystallized product is separated by filtration, washed twice with 5 ml hexane and dried under high vacuum. The reaction yield is about 89%. The physico-chemical properties of N-lauroyl-glycine methylester are as follows: -physical state: white crystalline powder -molecular formula: C-EH 2 QN0-3 -molecular weight: 271-40 -elemental analysis: C= 66.38% H= 10.77% N= 5-16% 0= 17-69% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble - 19 - -melting point: 62-64°C -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 Rf= 0.63 EXAMPLE 10: Preparation of N-palaitoyl-β-alanine 1.34g of β-alanine (15 mmol) are solubilized at e C in 40 ml of KOH IN. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.75 S of palmitoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, acidified with hydrochloric acid 6N and extracted three times with 20 ml ethyle acetate; the organic phases are washed twice with 15 ml water, collected, dried on anhydrous sodium sulphate and evaporated under vacuum. The crude product is crystallized from 30 ml of ter- butyl-methyl-ether. The crystallized product is separated by filtration, washed twice with 10 ml ter-butyl-methyl-ether and dried under high vacuum. The reaction yield is about 93%- The physico-chemical properties of N-palmitoyl-β-alanine are as follows: -physical state: white crystalline powder -molecular formula: -molecular weight: 37-51 -elemental analysis: C= 69-68% H= 11.39% N= 4.28% 0= 14.66% -solubility in org. sol.: >5 mg/ml in DMSO; >5 mg/ml in ethanol -water solubility: slightly soluble -melting point: 120 - 122°C -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 R : 0.64 - 20 - EXAMPLE 11: Preparation of N-palmitoyl-gamma-aminobutyric acid 1-55 g of gamma-amino-butyric acid (15 mmol) are solubilized at 4"C in 40 ml of KOH IN. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.75 g of palmitoyl- chloride (10 mmol). The resulting mixture is maintained under stirring at 0 β C overnight, acidified with hydrochloric acid 6N and extracted three times with 20 ml ethyle acetate; the organic phases are washed twice with 15 ml water, collected, dried with anhydrous sodium sulphate and evaporated under vacuum. The crude product is crystallized from 30 ml of ter-butyl-methyl-ether. The crystallized product is separated by filtration, washed twice with 10 ml ter-butyl- methyl-ether and dried under high vacuum. The reaction yield is about 90%. The physico-chemical properties of N-palmitoyl-gamma-aminobutyric acid are as follows: -physical state: white crystalline powder -molecular formula: C QH QNOO -molecular weight: 3^1. 4 -elemental analysis: C= 70.3^ H= 11.51% N= 4.10% 0= 14.05% -solubility in org. sol.: >5 mg/ml in DMSO; >5 mg/ml in ethanol -water solubility: slightly soluble -melting point: 104 - 106 β C -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 Rf: 0.64 EXAMPLE 12: Preparation of N,N' -bis- ( l-carboxy-2-hydroxyethyl ) - nonandi amide 4.66 g of L-serine methylester hydrochloride ( 30 mmol ) are solubilized - 21 - at 4"C in 80 isopropanole with 8.1 g of triethylamine (80 mmol). The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.25 g of azelaoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C for 2 h and at room temperature for a night and evaporated to dryness under vacuum. The crude product is suspended in 30 ml of water, extracted three times with 20 ml ethyle acetate; the organic phases are washed twice with 15 ml water, collected, and evaporated under vacuum. The crude product is suspended in 50 ml of water, under continuous stirring, and added with 20 ml NaOH IM to hydrolize the esteric groups. After 30 minutes the solution is cooled to 4°C and acidified with hydrochloric acid 6N. The precipitated crude is separated by filtration, crystallized from 30 ml of a cold mixture acetonitrile/water 9:1- The crystallized product is separated by filtration, washed twice with 5 ml acetonitrile and dried under high vacuum. The reaction yield is about 75%. The physico-chemical properties of N-N' -bis-( l-carboxy-2- hydroxyethyl)-nonandiamide are as follows: -physical state: white crystalline powder -molecular formula: j cH gN 2 0g -molecular weight: 362.38 -elemental analysis: C= 49-72% H= 7-23% N= 7-73% 0= 35-32 -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble (>10 mg/ml as sodium salt) -melting point: 89-93°C - 22 - -TLC: eluent: acetonitrile/water/acetic acid 4:1:0.1 Rf= 0.31 EXAMPLE 13: Preparation of N,N*-trans-2-dodecendioyl-diglycine 2.28 g of traumatic acid (10 mmol) are solubilized in 40 ml of anhydrous dimethylformamide at 0°C with 2 ml of pyridine. 2.53 g of N- hydroxysuccinimide (22 mmol) and 4.12 g of dicyclohexylcarbodiimide (20 mmol) are added and the resulting mixture is stirred for 2 h at 0"C. The formed urea is separated by filtration and discarded, while the solution containing the succinimidic ester is stirred again at 0 β C and added with 2.25 g of glycine (30 mmol) and 3-04 g of triethylamine (30 mmol) . The resulting mixture is stirred for a night at room temperature and evaporated to dryness under vacuum. The residue is solubilized in 50 ml of water and the solution is acidified with hydrochloric acid 6N. The crude precipitate is collected by filtration, dried under vacuum and crystallized from 60 ml of methanol. The crystallized product is separated by filtration, washed twice with 5 ml of methanol and dried under high vacuum. The reaction yield is 80%. The physico-chemical properties of N.N'-trans-2-dodecendioyl-diglycine are as follows: -physical state: white crystalline powder -molecular formula: 16 H 26 N 2°6 -molecular weight: 342.39 -elemental analysis C= 56.13% H= 7-65% N= 8.18% 0= 28.04 -solubility in org. sol.: >10 mg/ml in DMSO -water solubility: slightly soluble (>10 mg/ml as sodium - 23 - salt ) -melting point: 194 β C (dec) -TLC: eluent: acetonitrile/water/formic acid 4:1:0.1 Rf= 0.85 EXAMPLE 14: Preparation of N-palmitoyl-D-glucosamine 23 g of D-glucosamine hydrochloride (15 mmol) are solubilized at 4 β C in 60 ml of K 2 C0 IM. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.75 g of palmitoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0 β C overnight and acidified to neutrality with hydrochloric acid 6N. The precipitated crude product is separated by filtration and dried under vacuum; thereafter it is purified with repeated washing with warm ethanol, recovered by filtration and dried under high vacuum. The reaction yield is about 78%. The physico-chemical properties of N-palmitoyl-D-glucosamine are as follows: -physical state: white amorphous powder -molecular formula: ^22^43^6 -molecular weight: 417-58 -elemental analysis: C« 63.28% H= 10.38% N= 3-35% 0= 22.99% -solubility in org. sol.: >1 mg/ml in DMSO -water solubility: slightly soluble -melting point: 202°C (dec) -TLC: eluent: chloroform/methanol/water H 3 (28%) 80:25:2:1 Rf: 0.30 EXAMPLE 15: Preparation of N-lauroyl-D-glucosamine - 24 - 3-23g of D-glucosamine hydrochloride (15 mmol) are solubilized at 4°C in 60 ml of K 2 C0o IM. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.19 g of lauroyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0 β C overnight and acidified to neutrality with hydrochloric acid 6N. The precipitated crude product is separated by filtration and dried under vacuum; thereafter it is purified with repeated washing with warm isopropanol, recovered by filtration and dried under high vacuum. The reaction yield is about 85%. The physico-chemical properties of N-lauroyl-D-glucosamine are as follows: -physical state: white amorphous powder -molecular formula: -molecular weight: 361.48 -elemental analysis: C- 59-81% H= 9-76% N= 3.87% 0= 26.56% -solubility in org. sol.: >5 mg/ml in DMSO -water solubility: slightly soluble -melting point: >170 β C (dec) -TLC: eluent: toluene/ethanol/acetic acid 65:30:5 Rf: 0.31 EXAMPLE 16: Preparation of N-oleoyl-D-glucosamine 3.23 g of D-glucosamine hydrochloride (15 mmol) are solubilized at 4°C in 30 ml of K 2 C0o IM. The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 3-01 g of oleoyl chloride (10 mmol). The resulting mixture is maintained under stirring at 0°C overnight, acidified with hydrochloric acid 6M and extracted in continuous with ethyle acetate. The extraction solution is evaporated - 25 - to dryness and the crude product is purified by silica-gel chromatography, using as eluent a mixture of chloroform/methanol/water/NHo (25%) 85:15:1:0.5; the fractions containing the pure product are collected, evaporated to dryness and the residue is dried under high vacuum. The reaction yield is about 86%. The physico-chemical properties of N-oleoyl-D-glucoseamine are as follows: -physical state: white amorphous powder -molecular formula: C^H^c-NOg -molecular weight: 443-62 -elemental analysis: C= 64.98% H= 10.22% N= 3-16% 0= 21.64% -solubility in org. sol.: >5 mg/ml in DMSO -water solubility: slightly soluble -melting point: -TLC: eluent: chloroform/methanol/water NH^ (25%) 80:25:2:1 Rf: 0-35 EXAMPLE 17: Preparation of N-lauroyl-4-hydroxy-L-proline 1.97 g of 4-hydroxy-L-proline (15 mmol) are solubilized at 4'C in 50 ml of water containing 3-l8 g of Na 2 C0 (30 mmol). The solution is slowly added, dropwise under stirring over a period of 30 minutes, with 2.19 g of lauroyl-chloride (10 mmol) in 50 ml of petroleum ether. The resulting mixture is kept under stirring for 4 hours at 0°C and overnight at room temperature. The resulting mixture is acidified with HCl 6N and extrated three times with 50 ml ethyle acetate; the organic phases are washed 2 times with 15 ml of water, collected, dried with anhydrous sodium sulphate and evaporated under vacuum. The crude - 26 - product is crystallized from 50 ml of cycloesane. The crystallized product is separated by filtration, washed twice with 10 ml cycloesane and dried under vacuum. The reaction yield is about 85%- The physico-chemical properties of N-lauroyl-4-hydroxy-L-proline are as follows: -physical state: white crystalline powder -molecular formula: C 17 H 31 N0 4 -molecular weight: 313-44 -elemental analysis: C= 65-14% H= 9-97% N= 4.47% 0= 20.42% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble (>10 mg/ml in phosphate buffer 100 mM. pH 7-4) -melting point: 86.5-88.5°C -TLC: eluent: chloroform/methanol/water/NHo (28%) 80:25:2:1 Rf = 0.22 EXAMPLE 18: Preparation of N-palmitoyl-D-glucosamine tetra-0- emisuccinate 4.l8 g of N-palmitoyl-D-glucosamine (10 mmol) are solubilized at 4 β C in 40 ml of anhydrous pyridine; the resulting solution is added with 4.4 g of succinic anhydride (40 mmol) and kept under stirring at 0"C over 2 hours and overnight at 4θ β C. The solution is evaporated to dryness under vacuum, the residue is taken up with 20 ml HCl IN and extracted 3 times with 50 ml of ethyle acetate; the organic phases are washed 2 times with 15 ml of water, collected, dried with anhydrous sodium sulphate and concentrated under vacuum to a volume of about 50 - 27 - ml. The resulting solution is added with petroleum ether to muddiness and cooled at 4°C. The obtained crystallized product is separated by filtration, washed 2 times with 10 ml of petroleum ether and dried in high vacuum. The reaction yield is about 93%- The physico-chemical properties of N-palmitoyl-D-glucosamine tetra-0- emisuccinate are as follows: -physical state: white crystalline powder -molecular formula: ^38 R 9^l8 -molecular weight: 817-88 -elemental analysis: C= 55.8l% H= 7-27% N= 1.71% 0= 35-21% -solubility in org. sol.: >10 mg/ml in DMSO >10 mg/ml in ethanol -water solubility: slightly soluble (>10 mg/ml in phosphate buffer 100 mM, pH 7-4) -melting point: 131"C (dec.) -TLC: eluent: toluene/ethanol/acetic acid 80:20:2 Rf = 0.27 As already mentioned, the amides of mono and bicarboxylic acids with aminoalcohols and aminoethers according to the present invention, corresponding to formula (I) reported hereinabove. can selectively bind to CB2 peripheral receptor, acting as competitive agonists and displacing from said receptor the known natural and synthetic cannabinoids already known. Furthermore, said amides are capable of functionally activating CB2 receptor, by mimicking the non- psychoactive biological effects of cannabinoids and acting with a potency higher than or comparable with the one of cannabinoids. - 28 - The Applicant has also surprisingly found that CB2 cannabinoid peripheral receptor is present on mast cell and therefore the new compounds according to the present invention act on the same mast cell functionality. Furthermore, the Applicant has unexpectedly found that CB2 cannabinoid peripheral receptor is functionally expressed also on non-immune cells or tissue; in particular the Applicant has found that CB2 is functionally expressed in the Nervous System on cerebellar granule cells, indicating that CB2 receptor can modulate also non- immune cell function. This extremely important aspect of the present invention will be illustrated in detail hereinbelow. A) Identification of CB2 receptor on mast cells and non-immune cells. In order to verify that CB2 cannabinoid peripheral receptor is present on mast cells and non-immune cells (i.e. neurons), the presence of messenger RNA for said receptor was investigated in primary cultures of rat peritoneal mast cells, in a basophilic-mastocyte cell line from rat solid tumour (Rat Basophilic Leukemia, RBL-2H3) and in mouse cerebellar granule cell culture, in comparison with rat spleen homogenate tissues, where the presence of said receptor is acknowledged. Both Polymerase Chain Reaction and in situ hybridisation technique were used. i) Cannabinoid Peripheral Receptor specific hybridisation amplified by Polimerase Chain Reaction (PCR) . Extraction of total mRNA from tissues and cells Total RNA was extracted from rat peritoneal mast cells, RBL-2H3 cells and rat spleen, according to the method described by Chumczynski and - 29 - Sacchi (Anal. Bioche . , 162, 156-159. 1987). 5-10 mast cells, cells RBL-2H3 and 50 mg of spleen were separately homogenized in a 1.5 ml polypropylene tube Eppendorf**' (safe-lock type) with guanidinium isothiocyanate 5M (600 μl). Each tube was then supplied with sodium acetate 2M (60 μl), pH _ , and phenol (600 μl) saturated in buffer Tris- HCl IM, pH 7.0. The samples were briefly stirred by vortex mixing (Heidolpn, type REAX 2000 ®) and then added with chloroform-isoamyl alcohol (49:1 v/v) (120 μl). After agitation for 15 sec. by vortex mixing and incubation in ice for 1 min, the samples were centrifuged at 15,000xg at 4°C for 15 min. 600 μl of the upper aqueous phase was withdrawn and placed in another tube, having care to avoid any contact with the interface of the organic lower phase, which was discarded. The aqueous phase was added with isopropanol (600 μl) and, after agitation, the samples were incubated at -20°C for at least 60 min and centrifuged at 15,000xg at 4°C for 5 min. The supernatant was discarded. The pellet was washed with ethanol at 80% and then with absolute ethanol, concentrated to dryness and suspended again in water (20 μl) . 2 μl of the sample were used for the spectrophotometric determination of RNA concentration at the wave length of 260 nm and for the visualization with ethidium bromide on agarose gel at 1%. Reverse transcription and amplification by Polymerase Chain Reaction (PCR) of CB2 receptor 1 μg of total RNA withdrawn from each of the above samples was subjected to reverse transcription, as described by Leon at al. (PNAS, 91:3739-3743. 1994). using the reverse transcriptase of murine moloney leukaemic virus and 50 pmol of the synthetic oligonucleotide SEQ ID - 30 - N0:1, having sequence 5'-TAGGTAGGAGATCAAGCG-3' . complementary and antiparallel to the mRNA coding for cannabinoid peripheral receptor (Munro et al.. Nature 365, 6l- 65, 1993), in a total volume of 20 μl. After 1-hr reaction at 37°C, the volume of the tr-anscription product was brought to 100 μl with water and one fourth of the final volume was subjected to amplification by means of PCR. Said operation was conducted in 500 μl thin-walled test tubes, filled up to a final volume of 100 μl with a solution having the following composition: 50 mM TRIS HCl, pH 8.3. at 25°C: 75 mM KC1; 2.5 m MgCl 2 ; 10 mM DTT (dithiothreitol) ; 0.2 mM dATP, dCTP, dGTP and dTTP; 50 pmol sense primer SEQ ID NO:2 (5'-TTTCACGGTGTGGACTCC-3' ) ; 50 pmol antisense primer SΞQ ID NO:3 (5'-TAGGTAGGAGATCAAGCG-3' ) ; 0.551 Taq Pol (Taq polymerase) 2.5 U/μl (Polymerase Stoffel fragment, Cetus/Perkin-Elmei®) ; 3% formamide. Primers were specific for cannabinoid peripheral receptor. PCR was carried out for 35 thermal cycles in apparatus mod. 9600 of Cetus/Perkin Elmer®, according to the following procedure: Temperature Duration 95 β C 1 min 54"C 1 min 72°C 1 min Once the reaction had been completed. 30 μl of the reaction volume was electrophoresed on 1% agarose gel in order to visualize the - 31 - amplification products, then transferred and immobilized on a nylon filter for inner-probe hybridization to the two PCR primers. Specific hybridization with inner oligonucleotide The identity of the amplification product was determined by hybridization with a synthetic oligonucleotide SEQ ID N0:4, complementary to amplified sequences of cannabinoid peripheral receptor, labelled with radioactive tracers. The sequence SEQ ID NO:4 was as follows: 5'-GGTGACGAGAGCTrTGTAGGTAGGTGGGTAGCACAGACATAGGTA-3' Radioactive labelling was carried out at 37"C for 1 hr, using 5 pmol inner oligonucleotide, as follows: 33 pmol (α^ P)-dATP (New England Nuclear) ; 20 U Terminal Deoxynucleotide Transferase (US Biochemical); 100 mM sodium cacodylate (pH 7.2) 2 mM CoCl 2 0.2 mM 2-mercaptoethanol in a final volume of 20 μl. The labelled products were purified by chromatography on Sephadex G~5CN-^ column, and added to the hybridization solution containing the filter with the PCR-amplified products. Hybridization and washings were carried out under standard conditions, as described by Sambrook, Fritsch -and Maniatis (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1989)- The hybridized filter was exposed to a autoradiographic film for the detection of the amplified bands of the cannabinoid peripheral receptor messenger. - 32 - ii) m-RNA - Peripheral Cannabinoid Receptor in situ hybridisation. The brains of adult male mice (20-22 g) were frozen in 2-methyl-butane at -βO'C. sectioned with a cryostat and the 12 mm coronal sections were thaw-mounted on poly-L-lysine coated slides. All sections were then fixed in 4% paraformaldehyde (PFA) and dehydrated in a graded series of ethanol, including a min. incubation in chloroform and air dried. Mouse cerebellar granule cells (15 day in vitro-DIV) cultured on poly-L-lysine coated coverslips were fixed in 4% PFA, washed twice in PBS and permeabilized in 70% ethanol at 4°C for 48 hrs, dehydrated in higher graded ethanol and air dried. To detect the CB2-specific mRNA the synthetic oligonucleotide (45 mers) CB2 (SEQ ID N0:4: 5'-GGTGACGAGAGCTTTGTAGGTAGGTGGGTAGCACAGACATAGGTA-3 according to Munro et al., 1993. cited reference) was chosen. A random sequence was utilized for control sections. All oligonucleotides were tailed with ->S-dATP (NEN) using terminal deoxynucleotidyl-transferase (Pharmacia) to a specific activity of lθ" cpm/mg. All sections were hybridized in standard solutions (*) with 1.5 107 dpm/ml overnight at 42°C in a humidified chamber. Sections were then washed once in IX SSC/0.1%(**) SDS at 55°C for 30 min., then twice in IX SSC at 55°C for 15 min., followed by 0.1X SSC at 25°C for 30 min., rinsed in autoclaved water for 2min., dehydrated in ethanol and air dried. The slides were subsequently dipped in photoemulsion (Ilford K.5 diluted 1:1 in water), exposed for 5 weeks at 4"C, developed (Ilford Phenisol) , fixed (Ilford Hypam) and counterstained with cresyl violet. Standard Solution preparation (*) Hybridisation cocktail Formamide: 5 ml, 50%; SSC 20x: 2 ml, 4 x; - 33 - Denhardt's 50x: 0.2 ml; lx Lauryl-sacarosyl 20%: 0.5 ml, 1%; Destran sulphate: 1 gr, 10% ; 200 mM Phosphate buffer pH 7-0: 1 ml, 20 mM, water-DEPC q.s. to 10 ml. Filtrate using 0.45 m filter and store at - 20 β C . (**) Sodium Citrate Solution SCS 20x. Sodium chloride 175.3g and sodium citrate 88.2g were dissolved in 800ml DEPC-water; NaOH 10M was used to adjust the pH 7.2. Add DEPC-water q.s. to 1000ml for autoclave procedure. The autoradiographic examination shows that rat peritoneal mast cells and the cousin RBL-2H3 cells, cerebellar granule cells and cerebellum express, similarly to spleen preparations, mRNA specific to peripheral cannabinoid receptor CB2. This finding is clearly shown in Figures 1 and 2. Figure 1 illustrates the specific hybridization of cannabinoid peripheral receptor amplified by Polymerase Chain Reaction (PCR) respectively in (A) rat spleen, (B) rat peritoneal mast cell cultures and (C) RBL-2H3 cell cultures (C). Figure 2 illustrates the specific mRNA-CB2 peripheral cannabinoid receptor in situ hybridisation. B) Binding assays Specific binding assays were conducted for the purpose of checking whether the receptor was functionally expressed and whether the amides forming the object of the present invention were able to bind to CB2 receptor in a specific and competitive manner, compared with the cannabinoids having a known affinity to the receptor and compared with anandamide, the endogenous ligand of CBl receptor. a) Preparation of plasmatic membranes of RBL-2H3 cells RBL-2H3 cells (100 x 10 6 ) , frozen at -8θ°C. were thawed with 4 ml of Tris-HCl buffer solution 50 mM (pH 7-4), added with 0.25% w/v of - 34 - Tripsin inhibitor, type II-S: soy bean ^ (distributed by Sigma). The cells were resuspended in the buffer and homogenized. The homogenate was centrifuged at 1500xg at 4°C for 10 min. The supernatant obtained was collected and the precipitate was resuspended in 4 ml of the previous buffer. The resuspended precipitate was homogenized and centrifuged again at 1500xg at 4 β C for 5-10 min. The resulting supernatant was combined to the supernatant previously obtained and centrifuged at 5000xg for 10 min. After centrifugation, the supernatant was collected and further contrifuged at 40,000xg at 4°C for 30 min. The resulting precipitate was resuspended in 0.5 ml of the buffer solution described above, with addition of 1% Bovine Serum Albumin fatty acid free. The obtained suspension was centrifuged at 40,000xg at 4"C for 30 min. The resulting precipitate was collected and resuspended in buffer solution containing 50 mM Tris HCl, 1 mM Tris-EDTA, 3 mM MgCl 2 , pH 7.4, in order to obtain a proteic concentration of lμg/μl approx. This preparation may be used fresh, or frozen at -8θ°C and utilized within few days. b) "Binding" assays conditions to the preparation of RBL-2H3 cell membranes Saturation curve of labelled receptor agonist 3H-WIN 55,212-2 (specific activity 44 Ci/mmol, distributed by New England Nuclear): silanized plastic test tubes were fed, in the order, with binding buffer (50 mM Tris HCl. 1 mM Tris-EDTA, 3 mM MgCl 2 , 0.5% w/v Bovine Serum Albumin fatty acid-free) to a final volume of 0.5 ml, decreasing doses of 3H-WIN 55,212-2 from 0.5 to 20 nM (final) and 30 μg membrane proteins prepared in item a) . The resulting binding mixture was incubated at 30°C for 60 min under stirring and centrifuged at - 35 - 40,000xg at 20°C for 15 min. After centrifugation, an aliquot portion of the supernatant was collected to calculate the concentration of the ligand not associated to the membranes. After removal of the supernatant residue, the precipitate was washed with 1 ml PBS (Phosphate Buffer Solution) containing 0.5% Bovine Serum Albumin, taken up with 50 μl of a mixture of ethanol and 1% Triton X-100 (50/50 v/v), incubated at 37 * C for 20 min and resuspended. The resuspended material was added and mixed with scintillator liquid (3 ml) and placed in a "fS-counter" for 5 min. To evaluate the "Aspecific Binding", the predetermined test tubes were fed first with unlabelled receptor agonist WIN 55.212-2 at a final concentration of 15 M and then with the labelled ligand. Said binding gives Kd = 5-10 nM and Bmax = 100-250 pM. 3H-WIN 55.212-2 binding displacement For these competitions, 3H-WIN 55.212-2 was used at a concentration of 3 uM; non-radioactive WIN 55,212-2 (lμM) was used to inhibit specific binding. The competitors Nabilone [3~( 1.1-dimethylheptyl)- 6,6a,7,8,10,lOa-hexahydro-1-hydroxy-6,6-dimethyl~9H-dibenzo-[b,d]- pyran-9-one] and anandamide were solubilized in 0.1% ethanol, whereas the products forming the object of the present invention were solubilized in 0.1% DMSO. The competitors were added to the binding mixture prior to the labelled ligand. In both cases, for an evaluation of the reference specific binding, ethanol and DMSO were added at the same final concentration to the total binding and to the aspecific one. also in the absence of competitors. The aforementioned binding assays provided evidence that the receptor expression was complete and functional. - 36 - The data of Table 1 show that the amidic derivatives according to the present invention, like anandamide and the cannabinoid Nabilone, are able to bind to the receptor and to compete, with different potency, with the synthetic radioligand WIN 55.212-2. Table 1 - Competitive inhibition of |3H| WIN 55.212-2 specific binding by the compounds of the invention. |3H| WIN 55.212-2 was used at a concentration of 3 μM, whereas the other compounds were used at a concentration of 10 μM. Competitor Specific binding Displacement (%) 0.1% DMSO* 1433 — Example 1 — 100 Example 7 297 79 0.1% Ethanol** 1843 — Anandamide 99 95 Nabilone — 100 * 01.% DMSO is the concentration used for amides solubilization according to the present invention. ** 0.1% ethanol is the concentration used for Anandamide and Nabilone solubilization. - 37 - Table 2 - Competitive inhibition of 13Hj WIN 55.212-2 binding to RBL- 2H3 cell membranes by the amides of the invention and by the cannabinoids. Incubation was carried out in the presence of 1% DMSO. Inhibitory Concentration 50 values (ICπg) are expressed as means ± SEM, after the number of experiments indicated. Compound ^ E Q (nM) no. of experiments Nabilone 2.6±1.4 4 Δ 8-THC 223±120 6 Cannabidiol > 1000 3 Anandamide 33*2 5 Example 1 0.04;0.05 2 Example 7 0.16;0.005 2 Example 14 8.9*4.8 4 C) Biological activity assays The biological activity deriving from CB2 receptor functional activation was evaluated in RBL-2H3 cultures and in mouse cerebellar granule cell primary cultures. In particular, the ability of the amides of the invention to inhibit serotonin release, induced by specific stimulus, was evaluated on RBL cultures and compared with cannabinoids of known potency and with anandamide. The specific binding and/or the functional effects of the amidic derivatives described in Examples 1, 2, 5 _ H and 13-18 were evaluated. For purpose of comparison, the following products were used: among natural cannabinoids, three different isomers of tetrahydrocannabinol (THC) : Δ 8-THC, Δ 9-THC and 11-nor-Δ 8-THC- ; among synthetic cannabinoids. Nabilone and WIN 55.212-2, in addition to the CBl - 38 - endogenous ligand anandamide. Furthermore, it was shown that CB2 receptor plays a role in protecting against Excitatory Amino Acid (EAA)-induced cell death. Preparation of RBL-2H3 cell cultures RBL-2H3 cell cultures were grown in flasks (Falcon®), at 37°C, 5% C0 2 , in the presence of Minimum Essential Eagle's Medium (MEM) and 4 mM glutamine, 100 U/ml penicillin and 20% deactivated fetal calf serum (DFCS). RBL-2H3 cell sensitization and activation For each test, the cells were removed from the flasks with PBS, containing 0.5 m EDTA at pH 7.2. and seeded in wells (96 well plate Falconv ) in the presence of RPMI-1640 medium (code 6504, distributed by Sigma) containing 50 mg/1 gentamicin and 10% FCS at a density of 100,000 cells/100 μl medium/well. Furthermore, during seeding, said cells were loaded with serotonin by addition of 1 μCi/ml 3H-serotonin (5-hydroxy-triptamine 5HT, 26.4 Ci/mmol, New England Nuclear) and then incubated for 18 hrs (37°C, 5% C0 2 ). After 18-hr incubation in the presence of 3H-serotonin, the culture medium was removed and the cells were sensitized for 1 hr (37"C, 5% C0 ) in the presence of PIPES (N,N'-bis-(2-ethanesulphonyl)-piperazine, code 3768, Sigma) -buffered saline (100 μl/well), pH 7-1. containing 0.3 μg/ml mouse monoclonal antibody (IgE) against DNP (ADNP) . After removal of the sensitization medium, cells were activated - or not activated (control) - by addition, at 37°C for 155 min, of PIPES (100 μl/well), pH 7.1, containing - or not containing (control) - 0.1 μg/ml human albumin combined with dinitrophenyl (DNP-HSA) and containing a natural or synthetic cannabinoid or an amidic derivative - 39 - according to the present invention. After incubation, the medium was collected and centrifuged to determine the 3H-serotonin quantity released from DNP-HSA-stimulated or not stimulated cells. In parallel, adhered cells were solubilized with 1% Triton X-100 ® in PBS (100 μl/well) in order to determine the quantity of 3H-serotonin present in the cells. In both cases, the quantity of 3H-serotonin was measured by liquid scintillography and radioactivity counting, using a β-counter of Canberra Packard. 1900 TF® Addition of cannabinoids or of the derivatives according to the present invention during RBL-2H3 cells activation It was evaluated the effect of the amidic derivatives of the invention (stock solution in DMSO) and of cannabinoids in the presence or in the absence of anandamide (stock solution in ethanol) on DNP-HSA-induced 3H-serotonin release in DNP sensitized cells. To this purpose, cannabinoids or the amidic derivatives of the invention, with or without addition of anandamide, were added to the cell activation medium (PIPES ± DNP-HSA) at the desired concentration. In all cases, the medium was incubated at 37°C for 15 min, while the total final concentration was kept constant (0.2% solvents). The amides of the invention were solubilized in 0.2% DMSO and 0.1% absolute ethanol, except for N-palmitoyl-L-serine, which was solubilized in 1% DMSO and 0.1% ethanol. Quantification of 3H-serotonin net release after RBL-2H3 cells activation 3H-serotonin release in the various samples was calculated according to the following formula: - 40 - dpm released release = x 100 (dpm released + dpm associated with the cells) where "dpm" means nuclear disintegrations per minute. The effect of the amides of the invention was expressed as the percentage of 3H-serotonin net release (i.e. after deduction of the percentage of release in the absence of DNP-HSA) or as the percentage of 3H-serotonin release inhibition. The results of evaluation tests on the functional effects of cannabinoids and of the amidic derivatives of the invention are shown in Table 3- These data indicate that natural cannabinoids Δδ- tetrahydrocannabinol (Δδ-THC) and (Δ9-THC), the synthetic cannabinoid Nabilone and the labelled receptor agonist WIN 55.212-2 are able to inhibit, in a concentration-dependent way, RBL cells immunogenic activation measured as serotonin release. It is evident that the compounds described in Examples 1 and 7 can inhibit the effects induced by mast cell activation and exert an effect that is higher than or comparable to the one of cannabinoids. Conversely, anandamide is unable to affect serotonin release, thus demonstrating a specific structural selectivity in the receptor functional activation. The compounds derived from saturated carboxylic acids are more potent in exerting the functional effects following receptor activation and this is confirmed by the comparative tests results, reported below, wherein it is reported the effect of N- (2-hydroxyethyl )- hexadecanamide, wherein the acyl-moiety is sature, and that of N-(2- hydroxyethyl)-Inoleylamide. wherein the acyl-moiety presents two double bonds. - 41 Table 3 - Effect of natural and synthetic cannabinoids and of the amides according to the present invention on serotonin release induced by ADNP/DNP.HSA immunogenic stimulation in RBL-2H3 cells. The tested compounds were solubilized in 0.1% DMSO and 0.1% ethanol, a part from N-(2-hydroxyethyl)-hexadecanamide which was solubilized in 1% DMSO and 1% ethanol. Molecules added after 3H-serotonin net ED50 (μm) sensitization with ADNP release (%) DNP.HSA- 100 DNP.HSA+WIN 55.212-2 (50μM) 0 6.4 DNP.HSA+Nabilone (lOμM) 0 2.8 DNP.HSA+ Δ8-THC (25μM) 0 5 DNP . HSA+ 19-THC (25μM) 27 5 DNP . HSA+ll-nor-Λ8-THC-9 (20μM) 33 15 DNP.HSA+anandamide 100 — DNP.HSA+N-(2-hydroxyethyl)- -hexadecanamide (lOOμM) 0 2.6 DNP.HSA+N-(2-hydroxyethyl)- -linoleylamide (lOOμM) 100 — DNP.HSA+Example 1 (lOOμM) 0 — DNP.HSA+Example 7 (10μM) 27 4.8 Co-incubation of anandamide with natural or synthetic cannabinoids and with the derivative described in Example 7 reduces the ability of the acyl amide of the invention, as well as of cannabinoids, to inhibit serotonin release, as reported in Table 4. - 42 - Table 4 - Anandamide antagonism towards the inhibitory effect of cannabinoids and amidic derivatives of the invention on ADNP/DNP.HSA- induced activation in RBL-2H3 cells. Molecules added after 3H-serotonin net release sensitization with ADNP inhibition ( % ) -anandamide + anandamide (12.5 μM) DNP.HSA+WIN 55,212-2 (30μM) 100 36 DNP.HSA+Nabilone (5μM) 90 50 DNP.HSA+Δδ-THC (25μM) 100 n . d DNP.HSA+Δ9-THC (25μM) 73 n. d DNP.HSA+ll-nor-Δ8-THC-9 (20μM) 67 n. d DNP.HSA+Example 7 (10μM) 73 24 The data reported in Table 5 show that the co-incubation of anandamide with natural or synthetic cannabinoids and with the amidic derivatives according to the present invention reduces the potency of anandamide, indicating that anandamide causes a competitive partial antagonism on the peripheral receptor. Therefore, as shown in Table 1, anandamide, though having binding affinity for the peripheral receptor, is not able to induce biological effects and, by competing for the receptor, functionally antogonizes the protective effects of cannabinoids. - 43 - Table 5 _ EDCQ of cannabinoids and of the amidic derivatives of the invention on the inhibition of 3H-serotonin release induced by ADNP/DNP.HSA stimulation in RBL-2H3 cells, in the presence and in the absence of anandamide. Molecules ED50 (μM) -anandamide +anandamide (12.5 μM) WIN 55.212-2 6.4 40 Nabilone 2.8 5 Example 7 4.8 >100 Example 14 O.51 3- 30 Example 16 2.90 4.20 The partial competitive antagonism of anandamide on the peripheral receptor is exerted by the integral molecule and not by anandamide metabolites, as shown in Table 6. - 44 - Table 6 - 3H-serotonin release inhibition by WIN 55.212-2 (used at a concentration of 30 μm): antagonistic effect of anandamide vs. the metabolites of the same. 3H-serotonin net release inhibition (%) -WIN +WIN DNP.HSA 0 69 DNP.HSA + 12.5μM anandamide 7 15 DNP.HSA + 12.5μM ethanolamine 6 72 DNP.HSA + 12.5μM arachidonic acid 4 59 DNP.HSA + 12.5μM ethanolamide + 12.5μM arachidonic acid 3 6l The tests results reported in Tables 4, 7 and 8 show that the receptorial specificity and the level of activity are functional to the nature of both the acylic and the amidic moieties of the amidic derivatives according to the present invention. - 45 Table 7 - Inhibitory effect of the amidic derivatives according to the present invention on 3H-serotonin release induced by ADP/DPN . HSA immunogenic stimulation in RBL-2H3 cells . Compounds 3H-serotonin release inhibition % DMSO 0.2% DMSO 1% Example 2 79 (lOOμM) 61 (lOOμM) Example 5 61 (lOOμM) Example 6 50 ( 6θμM) 91 (lOOμM) Example 7 85 ( 6θμM) 100 (lOOμM) Example 8 93 (100μM) 100 (lOOμM) Example 9 55 (lOOμM) Example 10 47 (lOOμM) Example 13 54 (lOOμM) Example 14 76 ( 20μM) 91 (lOOμM) Example 15 67 ( 20μM) 86 (lOOμM) Example 16 92 ( 20μM) 86 (lOOμM) Numbers in paretheses are the concentrations (μM) used for the various compounds in the different tests. - 46 - Table 8 - Inhibition of |3H| serotonine release from DNP-HSA activated RBL-2H3 cells. The compounds of the invention were present during the 15 minutes release period. EC50 is the concentration inhibiting by 50% the net release of |3H| serotonin from DNP-HSA - activated RBL-2H3 cells. The reported values are expressed as means ± SEM, after the number of experiments shown in parentheses. Compound EC50 0.2% DMSO 1% DMSO Example 5 104 ± 59 (2) Example 6 29 ± 12 (3) Example 8 10 ± 1.5 (3) 7 ± 5 (2) Example 10 30 (1) Example 11 26 ± 18 (4) Example 14 1.28±0.38 (6) 0.12±0.07 (3) Example 15 12 (1) Example 16 2.4 * 0.5 (4) Cannabinoid neuroprotective effect in cerebellar granule cell culture Granular cell cultures were obtained from postnatal day 8-9 mouse Balb-6 cerebellum. The cells were suspended in EBM + 2mM L-glutamine, 100 U/ml penicillin, 50μg/ml gentamycin, 25mM KCl and 10% fetal calf serum, plated on polylisine substrate, cultured from 8-10 days and then exposed to glutamate (500 μM) for 5 minutes at room temperature, exiting in 60% cell death. Cannabinoids and anandamide were solubilized in Locke's solution and added to the culture for 10 minutes, starting 15 minutes after removing glutamate. The number of - 47 - survived cells was calculated by the colorimetric method MTT, 24 hours after the washing out of thr compound. Cannabinoids protect against cell death induced by glutamate. To the contrary, anandamide, which can not functionally activate CB2 cannabinoid peripheral receptor, does not display any protective effect, as shown in Table 9; anadamide was not effective up to 100 μM. Furthermore, the data reported in Table 9 show that CB2 receptor is functionally expressed also on neurons culture, being able to play a protective role on cell survival; this indicates that CB2 receptor capability of modulating events is non-directly mediated by immune lineage cell. Table 9 _ Cannabinoid neuroprotective effect in comparison with anandamide. The reported values are expressed as means ± SEM, after the number of experiments indicated in parentheses. Compound EC50 Nabilone 3.90 ± 1.1 (3) Anandamide no effect (3) The experimental data reported above prove a precise structure- activity correlation in the selective activation of CB2 peripheral receptor, which mediates the non-psychoactive effects of cannabinoids. More precisely, the molecules having a saturated acylic chain are able to bind and, above all, to functionally activate CB2 peripheral receptor to a significant extent. To the contrary, compounds with more than one double bond in the acyl chain, though having affinity to CB2 receptor, are not able to activate said receptor functionally, thus acting as partial competitive receptor antagonists. - 48 - Furthermore, the aforementioned experimental results demonstrate that mast cells express the cannabinoid peripheral receptor and that the amidic derivatives according to the present invention behave as competitive agonists of CB2 receptor with an affinity that is higher than, or in any case comparable with the one of natural and synthetic cannabinoids. Moreover, they functionally activate said receptor. Importantly, the Applicant has found that also non-immune cells functionally express the cannabinoid peripheral receptor CB2, which for example was observed in cerebellar granule cells, cerebellum, heart and lung. Said results are extremely interesting from a therapeutic point of view: in fact, the amides of the invention are suitable for the treatment of all diseases for which cannabinoids are notoriously efficacious, acting only on CB2 peripheral receptor and excluding the activity mediated by CBl central receptor, responsible for untoward effects. It is also very important the fact that the amidic derivatives according to the invention can directly or indirectly regulate the defence response to various noxae involving both cell types belonging to the immune lineage and neurons or cells having different physiological function, i.e. heart and lung, by modulating mast cell degranulation through the selective activation of CB2 receptor. The importance of mast cells in inflammatory processes connected with several acute and chronic pathologies is well known in the state of the art (Otten U. et al.. Nerve Growth Factor induces growth and differentiation of human B lymphocytes, PNAS, 86:10059-10063, 1989; Marshall J. S. et al., 1989, The J. of Immunology, 144:1866-92; B: - 49 - Henderson and S. Blake, Therapeutic potential of cytokine manipulation, TIPS, 13,145-152, 1992; M.C.Morganti-Kossmann et al., Cytokines -and neuropathology, TIPS. 13. 286-290, 1992; A.S. Fauci, Science, 262, 1011- 1018, 1993; E.A. Formukong at al., Analgesic and Antiinflammatory Activity of Constituents of Cannabis Sativa L. , Inflammation, 12, 4:361- 371, 1988; W.D. Lyman, Drugs of Abuse and Experimental Autoimmune Diseases, Exp. Med. Biology, 288:81-82, 1991; G.H. Gibbons et al.. The Emerging Concept of Vascular Remodeling, Mechanisms of Disease, 330. 20: 1431-1438, 1994; P.T. Kovanen, The mast cell, a potential link between inflammation and cellular cholesterol deposition in atherogenesis, Europen Heart Journal, 14 suppl. K: 105-117. 1993)- In other words, thanks to their specific characteristics, the amidic derivatives of the invention are able to selectively bind and to functionally activate CB2 cannabinoid peripheral receptor. Therefore, they represent an important therapeutic tool both for the treatment of pathologies connected with the modulation of CB2 cannabinoid peripheral receptor and for the treatment of the pathologies deriving, for example, form mast cell degranulation influenced by CB2 receptor. Therefore, the therapeutic use of said compounds is particularly useful in the treatment of diseases connected with an anomalous modulation of CB2 peripheral receptor, or that benefit of the activation of said receptor with consequent negative modulation of cytotoxic or proinflammatory phenomena, connected with cytokine, neurokine or enzyme release, or with second messenger activation, such as: - 50 - - pathologies connected with the immune system alteration; - pathologies with etiologic autoimmune component, such as multiple sclerosis, lateral amyothrophic sclerosis, and the associated muscolar spasm; - articular acute and chronic inflammatory pathologies, also on autoimmune basis, such as rheumatoid arthritis; - pathologies caused by biological agents such as viral (HIV) and bacterial encephalitic meningitis, bacterial meningitis, meningitis by cytomegalovirus and AIDS-dementia complex; - chronic neurodegenerative pathologies, such as senile dementia, Alzhaeimer's and Parkinson's diseases, and the associated cachectic symptomatology; - pathologies with nociception alteration; - pathologies associated with EAA excitotoxicity such as cerebral ictus, TIA, cerebral and spinal cord trauma, epilepsy, migraine and corea; - cardiovascular pathologies associated with vascular remodelling i.e. restenosis after angioplasty including stent application, atherosclerosis and heart attack; " pathologies involving pressure alterations (hypertension) at cardiovascular, pulmonary and ocular levels, i.e. glaucoma; - chronic airway obstruction, including asthma; - nausea, also of hyatrogenic nature. In view of their effects, the amidic derivatives according to the present invention are suitable for the treatment of human and animal diseases. For all aforesaid diseases, the systemic administration of the claimed - 51 - compounds by the oral or parenteral or topical or transdermic ways may be envisaged. The therapeutically effective dose varies depending on the way of administration, as well as on the disease seriousness; it also varies depending on the patient's age, weight and general health conditions. In any case, acceptable therapeutic doses may range from 0.1 to 20 mg/kg/die, over variable periods, in any case for at least 30 days. The pharmaceutical compositions containing as the active principles the amides according to the present invention are inclusive of all formulations containing pharmaceutically acceptable excipients, that are suitable for the administration of the active ingredients in the forms best suited to the disease to be treated and, in any case, rendering the active ingredient as bioavailable as possible. In particular, solutions or suspensions for general i.v., s.c. and i.m. administration, solutions for ophthalmic treatment in the form of eyewash, solid or semisolid formulations in the form of inserts, gels and ointments are to be envisaged. As concerns oral formulations, granular powders, tablets, pills and capsules will be preferred. As concerns dermic and transdermic administration, creams, ointments, gels and plasters, where the active ingredient may be included in slow-releasing microspheres, will be preferred. EXAMPLE 19 Vials for injection Every vial contains: Compound of Example 8 20 mg Mannitol 12 mg Sodium metasulphite 1.3 mg - 52 - Benzyl alcohol 60 mg Propylene glycol 400 mg Sodium hydroxide q.s. to pH 7 Water for injectable formulations q.s. to 2 ml EXAMPLE 20 Tablets Every tablet contains: Compound of Example 1 50 mg Dibasic dihydrated calcium phosphate 135-2 mg Microgranular cellulose 36 mg Maize starch 7-2 mg Magnesium stearate 1.8 mg Hydrogenated vegetable oil 1.2 mg Precipitated silica 0.6 mg Hydroxypropylene ethylcellulose 4.7 mg Titanium dioxide 0.3 mg EXAMPLE 21 Eyewash Every bottle contains: Compound as per Example 7 25 mg Borax 15 mg Boric acid 75 mg Polysorbate 80 15 mg Lactose 80 mg Phenol 3-9 mg Disodium edetate 5 mg Water for injectable formulation q.s. to 5 ml - 53 EXAMPLE 22 Soft capsules Every capsule contains: Compound of Example 16 100 mg Excipient: peanut oil O.P. 100 mg Composition of the capsule: gelatin O.P., glycerin O.P., natural dye E12

Claims: - 54 - CLAIMS 1. Amide of formula (I) 0 R 2 II / R χ - C - N (I) R 3 wherein R^ can be: 1) a linear or branched hydrocarbon radical having from 9 to 23 carbon atoms, saturated or presentig one double bond, optionally substituted with one or more -OH groups; 2) a group of formula (II): R 6 wherein R/ j is a linear hydrocarbon radical, saturated or containing one double bond, comprising from 8 to 22 carbon atoms, optionally substituted with one or more linear or branched alkyl groups C-^-Cg and/or with one or more -OH groups; 3) a group of formula (IV): 0 II - Riψ - C - 0 - Ry (IV) wherein R^ has the meanings decribed above and Ry = -H or a linear or branched alkyl group, comprising from 1 to 20 carbon atoms; the residues od formulae: / , R 2 / , R 5 (III ) and - N (V) \ \ R 3 R 6 where R 2 and RT are equal or different from R and R Q , are chosen in - 55 - one of the following classes : A) a residue of an optically active or non active aminoacid , wherein 2 and Rr , together with the nitrogen atom to which are bound, form an α-aminoacid , optionally presenting an aliphatic or arylaliphatic lateral chain optionally substituted with -OH , -0P0 H 2 , -0-P0 2 H-0- CH 2 -CH ( 0H ) -CH 2 -0H . -SH . -S-CHo ; or R 2 and R 5 , together wi th the nitrogen atom to which are bound , a β- or -aminoacid presenting an aliphatic or arylaliphatic lateral chain ; the acid group of such aminoacid being optionally methylated or ethylated ; R and R^ are -H or -CH ; when R^ belongs to class (1) and R 2 forms, together with the N atom to which it is bound, the aminoacylic residue of alanine, β-alanine or glycine, R is different from -CH ; B) a residue of a glycosamine having formula: wherein one of the groups P, Y and Z is -N-C0-R j _ and the R 3 other groups can be -H or -OH, R-^ being as above described and R = -H the residues R 2 , R ? . Rr -and/or Rg of formulae (III) and (V) being optionally substituted with at least a pharmaceutically acceptable radical capable of increasing the solubility of the amide of formula (I). 2. The amide according to claim 1, characterised in that, when R-^ - 56 - belongs to class (1), it is a radical having from 11 to 17 carbon atoms. 3- The amide according to claim 1, characterised in that, when Ri belongs to class (2) , R^ is a radical containing from 10 to 16 carbon atoms. 4. The amide according to claim 1, characterised in that the residues R 2 , R , Re and/or Rg in formulae (III) and (V) are substituted with at least a pharmaceutically acceptable radical selected from the group consisting of acyls, emiacyls of bicarboxylic acids, dialkylamino- acyls, alkyl-sulphonates, alkyls, 0-phosphates and 0-sulphates. 5- The amide according to claim 4, characterised in that said acyl is acetyl or benzoyl. 6. The amide according to claim 4, characterised in that said emiacyl of a bycarboxylic acid is the emiacyl of succinic or glutaric acid. 7- The amide according to claim 4, characterised in that said alkyl- sulphonate is metansulphonate or p-toluen-sulphonate. 8. The amide according to claim 4, characterised in that said alkyl is methyl or ethyl. 9- The amide according to claim 1, characterised in that, when R^ belongs to class (1), it forms, together with the adjacent carbonyls, the acyl group of a monocarboxylic acid selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, palmitoleic acid, oleic acid and^-hydroxy-palmitic acid. 10. The amide according to claim 1, characterised in that, when R-^ belongs to classes (2) and (3). R4 forms with the two adjacent carbonyls the acyl group of traumatic acid. 11. The amide according to claim 1, characterised in that, when the - 57 - residues of formula (III) and (V) belongs to class (A) , they are the residue of an aminoacid selected from the group consisting of glycine, β-alanine, V-amino-butyric acid, serine and cysteine. 12. The amide according to claim 1, characterised in that, when the residues of formula (III) and (V) belongs to class (B), said glycosamine is selected from the group consisting of D-Glucosamine, L-Acosamine, D- Mannosamine and D-Galactosamine. 13- Process for the preparation of amides of formula (I): 0 R-, II / R-, - C - N (I) \ 3 wherein R-^ can be: 1) a linear or branched hydrocarbon radical having from 9 to 23 carbon atoms, saturated or presentig one double bond, optionally substituted with one or more -OH groups; 2) a group of formula (II): 0 Rr II / 5 - R 4 - C - N (II) R 6 wherein R^ is a linear hydrocarbon radical, saturated or containing one double bond, comprising from 8 to 22 carbon atoms, optionally substituted with one or more linear or branched alkyl groups C^-Cg and/or with one or more -OH groups; 3) a group of formula (IV): 0 II - R 4 - C - 0 - Ry (IV) wherein R^ has the meanings decribed above and Ry = -H or a linear or - 58 - branched alkyl group, comprising from 1 to 20 carbon atoms; the residues of formulae / , R 2 / , R 5 - N ( III ) and - N (V) \ \ R 3 R 6 where R and R? are equal or different from Rr and Rg, are chosen in one of the following classes: A) a residue of an optically active or non active aminoacid, wherein R 2 and Re, together with the nitrogen atom to which are bound, form an α-aminoacid, optionally presenting an aliphatic or arylaliphatic lateral chain optionally substituted with -OH, -0P0 H 2 , -0-P0 2 H-0- CH 2 -CH(0H)-CH 2 -0H, -SH, -S-CH ; or R 2 and Rr form, together with the nitrogen atom to which are bound, a β- or v -aminoacid presenting an aliphatic or arylaliphatic lateral chain; the acid group of such aminoacid being optionally methylated or ethylated; R and Rg are -H or -CH-,; when R^ belongs to class (1) and R 2 forms, together with the N atom to which it is bound, the aminoacylic residue of alanine, β-alanine or glycine, R is different from -CH^; B) a residue of a glycosamine having formula: wherein one of the groups P, Y and Z is -N-CO-R j and the R 3 other groups can be -H or -OH; R-, being as above described and = -H - 59 - or -CH , the residues R 2 , R?, Rr and/or Rg of formulae (III) and (V) being optionally substituted with at least a pharmaceutically acceptable radical capable of increasing the solubility of the amide of formula (I); comprising the reaction of an activated form of the acid R..-C00H, of formula R-^-CO-X, with an amine of formula H - N Λ \ R 3 in aqueous or organic solvents in the presence of a base , under stirring at a temperature ranging from -20° to - 6θ°C, for a period ranging from 10 minutes to 24 hours ; the optional acidification of the reaction medium; the isolation and the purification by conventional techniques . 14. The process according to claim 13, characterised in that said temperature is comprised between -10° and 20°C. 15- The process according to claim 13, characterised in that the reaction time is comprised between 1 hour and 3 hours. l6. The process according to claim 13. characterised in that said activated form of the acid is selected from the group consisting of acyl halide. succinimidylester, acylisourea, p-N0 ~phenylester and methylester. 17. The process according to claim 13. characterised in that said base is selected from the group consisting of K 2 C0τ , KOH, Et-jN and N- methyl- morpholine. 18. Use in the therapeutic treatment of human and animal pathologies - 60 - connected with the modulation of CB2 peripheral receptor, and with the degranulation and/or lysis of immunocompetent cells controlled by CB2 receptor, of amides of formula (I): R 3 wherein R-^ can be: 1) a linear or branched hydrocarbon radical having from 9 to 23 carbon atoms, saturated or presentig one double bond, optionally substituted with one or more -OH groups; 2) a group of formula (II): wherein R^ is a linear hydrocarbon radical, saturated or containing one double bond, comprising from 8 to 22 carbon atoms, optionally substituted with one or more linear or branched alkyl groups C^-C and/or with one or more -OH groups; 3) group of formula (IV): 0 II - R^ - C - 0 - Ry (IV) wherein _. has the meanings decribed above and Ry = -H or a linear or branched alkyl group, comprising from 1 to 20 carbon atoms; the residues of formulae / , R 2 / , R 5 N ( III) and - N (V) \ \ R 3 R 6 61 - where R 2 and R are equal or different from Rr and Rg, are chosen in one of the following classes: A) a residue of an optically active or non active aminoacid, wherein R 2 and Rr, together with the nitrogen atom to which are bound, form an α-aminoacid, optionally presenting an aliphatic or arylaliphatic lateral chain optionally substituted with -OH, -0P0 3 H 2 , -0-P0 2 H-0- CH 2 -CH(0H)-CH 2 -0H, -SH, -S-CHo; or R 2 and R^ form, together with the nitrogen atom to which it is bound, a β- or ^-aminoacid presenting an aliphatic or arylaliphatic lateral chain; the acid group of said aminoacid being optionally methylated or ethylated; R and Rg are -H or -CHo; B) a residue of a glycosamine having formula: wherein one of the groups P, Y and Z is -N-CO-R-^ and the R 3 other groups can be -H or -OH; R-^ being as above described and R = -H or -CH 3 ; the residues R 2 , Ro, Rr and/or Rg of formulae (III) and (V) being optionally substituted with at least a pharmaceutically acceptable radical capable of increasing the solubility of the amide of formula (I). 19 - The use according to claim 18 , characterised in that said human pathologies are pathologies connected with the immune system alteration. - 62 - 20. The use according to claim 18, characterised in that said human pathologies are pathologies with etiologic autoimmune component, selected from the group consisting of multiple sclerosis, lateral amyothrophic sclerosis and the associated muscolar spasm. 21. The use according to claim 18, characterised in that said human pathologies are articular acute and chronic inflammatory pathologies. 22. The use according to claim 21, characterised in that said chronic inflammatory pathology is rheumatoid arthritis. 23- The use according to claim 18, characterised in that said human pathologies are pathologies caused by biological agents selected from the group consisting of viral (HIV) and bacterial encephalitic meningitis, bacterial meningitis, meningitis by cytomegalovirus and AIDS-dementia complex. 24. The use according to claim 18, characterised in that said human pathologies are chronic neurodegenerative pathologies select3d from the group consisting of senile dementia, Alzhaeimer's <and Parkinson's diseases, and the associated cachectic symptomatology. 25. The use according to claim 18, characterised in that said human pathologies are pathologies with nociception alteration. 26. The use according to claim 18, characterised in that said human pathologies are pathologies associated with EAA excitotoxicity, selected from the group consisiting of cerebral ictus, TIA, cerebral and spinal cord trauma, epilepsy, migraine and corea. 27- The use according to claim 18, characterised in that said human pathologies are cardiovascular pathologies associated with vascular remodelling, atherosclerosis or heart attack. 28. The use according to claim 18, characterised in that said human - 63 - pathologies are pathologies involving pressure alterations at cardiovascular, pulmonary or ocular level. 29- The use according to claim 28, characterised in that said pathology is glaucoma. 30. The use according to claim 18, characterised in that said human pathologies are chronic airway obstructions. 31- The use according to claim 30, characterised in that said pathology is asthma. 32. The use according to claim 18, characterised in that said human pathology is nausea. 33- A pharmaceutical composition containing as the active principle a therapeutically active amount of amides of formula (I): 0 R-> II / R χ - C - N (I) R 3 wherein R-^ can be: 1) a linear or branched hydrocarbon radical having from 9 to 23 carbon atoms, saturated or presentig one double bond, optionally substituted with one or more -OH groups; 2) a group of formula (II): 0 Rr II / 5 - R - C - N (II) \ R 6 wherein R^ is a linear hydrocarbon radical, saturated or containing one double bond, comprising from 8 to 22 carbon atoms, optionally substituted with one or more linear or branched alkyl groups C^-Cg and/or with one or more -OH groups; - 64 - 3) a group of formula (IV): 0 II - R 4 - C - 0 - Ry (IV) wherein R^ has the meanings decribed above and Ry = -H or a linear or branched alkyl group, comprising from 1 to 20 carbon atoms; the residues of formulae: - N Λ (III) and - N (V) \ \ R 3 R 6 where R and Ro are equal or different from Re and R^, are chosen in one of the following classes: A) a residue of an optically active or non active aminoacid, wherein R 2 and Ro, together with the nitrogen atom to which are bound, form an α-aminoacid, optionally presenting an aliphatic or arylaliphatic lateral chain optionally substituted with -OH, -0P0oH 2 , -0-P0 2 H-0- CH 2 -CH(0H)-CH 2 -0H, -SH, -S-CHo; or R 2 and Rr form, together with the nitrogen atom to which are bound, a β- or V -aminoacid presenting an aliphatic or arylaliphatic lateral chain; the acid group of said aminoacid being optionally methylated or ethylated; 3 and Rg are -H or -CHo; B) a residue of a glycosamine having formula: wherein one of the groups P, Y and Z is -N-CO-R-^ and the Ro - 65 - other groups can be -H or -OH; R^ being as above described and Ro = -H or -CH^; the residues R 2 , Ro. Rr and/or .c of formulae (III) and (V) being optionally substituted with at least a pharmaceutically acceptable radical capable of increasing the solubility of the amide of formula (I); in combination with pharmaceutically acceptable excipients. 34- The pharmaceutical composition according to claim 33. wherein said active principle is administered in quantities ranging from 0.1 and 20 mg/kg/die. 35- The pharmaceutical composition according to claim 33. characterised in being administered by oral, parenteral, topical or transdermical route. 36. The pharmaceutical composition according to claim 35. characterised in that said parenteral route is endovenous, subcutaneous or intramuscular route, and said composition is in the form of injectable solution or suspension. 37- The parmaceutical composition according to claim 35. characterised in that said topic route is the ophtalmic route, and said composition is in the form of eyewash, solid or semi-solid formulation in the form of insert, gel or ointment. 38. The pharmaceutical composition according to claim 35. characterised in being orally administered in the form of granular powder, tablets, dragees or capsulas. 39- The pharmaceutical composition according to claim 35. characterised in being dermically or transdermically administered in the form of cream, ointment, gel or plaster, said active principle being optionally contained is slow-releasing microspheres. - 66 - 40. The pharmaceutical composition according to claim 33. usefull in the therapeutic treatment of human and animal pathologies connected with the modulation of cannabinoids peripheral receptor CB2, or with the degranulation and/or lysis of immunocompetent cells controlled by CB2 receptor.

Inventors: DELLA VALLE FRANCESCO, LEON ALBERTA, LORENZI SILVANA, MARCOLONGO GABRIELE, DELLA VALLE, FRANCESCO, LEON, ALBERTA, LORENZI, SILVANA, MARCOLONGO, GABRIELE
Applicants: DELLA VALLE FRANCESCO, LEON ALBERTA, LIFEGROUP SPA, LORENZI SILVANA, MARCOLONGO GABRIELE, DELLA VALLE, FRANCESCO, LEON, ALBERTA, LIFEGROUP S.P.A., LORENZI, SILVANA, MARCOLONGO, GABRIELE
Application year: 1995
Main classification: C07C223
IPC codes: A61K31, A61P9, A61P11, A61P25, A61P27, A61P29, A61P31, A61P37, C07C233, C07C323, C07D207, C07H13