ADENO-ASSOCIATED DERIVED VECTOR SYSTEMS FOR GENE DELIVERY AND INTEGRATION INTO TARGET CELLS

Abstract: A novel nucleotide sequence integration and targeting system is described. The system employs adeno-associated virus (AAV) derived vectors which include a selected nucleotide sequence bounded by AAV inverted terminal repeats (ITRs). An AAV rep coding region is also provided, enabling the targeted integration of the selected nucleotide sequence into the genome of a suitable target cell. The nucleotide sequence integration system of the present invention can deliver and integrate large segments of DNA into the genome of target cells. Further, the subject integration system provides the advantage of site-specific integration of the selected nucleotide sequences in a target cell genome, thereby avoiding insertional mutagenesis events experienced with prior systems.

Description: ADENO-ASSOCIATED DERIVED VECTOR SYSTEMS FOR GENE DELIVERY AND INTEGRATION INTO TARGET CELLS TECHNICAL FIELD The present invention relates generally to vectors for gene delivery and integration More specifically, the invention relates to adeno-associated virus (AAV) derived vector systems for use in gene delivery which provide for integration of a selected nucleotide sequence into a target cell genome. BACKGROUND OF THE INVENTION Gene delivery is a promising method for the treatment of acquired and inherited diseases A number of viral based systems are being developed for gene transfer purposes In particular, retroviruses are currently the most widely used viral vector system for gene delivery Retroviral systems generally employ packaging lines which have an integrated defective provirus (the "helper") that expresses all of the genes of the virus but cannot package its own genome due to a deletion of the packaging signal Thus, the cell line produces empty viral shells. Producer lines can be derived from the packaging lines which, in addition to the helper, contain a viral vector which includes sequences required in cis for replication and packaging of the virus, known as the long terminal repeats (LTRs) The selected gene can be inserted in the vector and packaged in the viral shells synthesized by the retroviral helper. The recombinant virus can then be isolated and delivered to a subject For descriptions of various retroviral systems, see, e.g., U.S. Patent No. 5,219,740; Miller and Rosman (1989) BioTechniques 7:980-990; Miller, A.D. (1990) Human Gene Therapy I 5-14, Scarpa et al. (1991) Virology 180:849-852; Burns et al. (1993) Proc. Nail. Acad. Sci. USA 90:8033-8037; and Boris-Lawrie and Temin (1993) Cur. Opin. Genet. Develop. 3 102-109 Despite their popularity, retroviral systems suffer from several drawbacks In particular, retroviral particles are relatively labile and hence unstable Therefore, purification of recombinant viruses can lead to significant loss in titer Furthermore, retroviruses have a limited host range and cannot integrate into nonreplicating cells Accordingly, cells which do not normally divide, such as mature neurons, or cells which replicate slowly, cannot be genetically altered using retroviral vectors unless stimulated to divide before infection Additionally, and importantly, retroviruses are known to cause disease in certain animals, including humans, and thus pose a significant health risk to the subject transfected with a recombinant virus Finally, retrovirus vectors integrate into the host cell chromosome randomly, which may cause insertional mutagenesis by activating oncogenes or inactivating tumor suppressor genes Adenovirus based systems have been developed for gene delivery in an attempt to overcome these problems. Human adenoviruses are double-stranded DNA viruses which enter cells by receptor-mediated endocytosis These viruses are particularly well suited for gene transfer because they are easy to grow and manipulate and they exhibit a broad host range in vivo and in vitro For example, adenoviruses can infect human cells of hematopoietic, lymphoid and myeloid origin. Furthermore, adenoviruses infect quiescent as well as replicating target cells Unlike retroviruses which integrate into the host genome, adenoviruses persist extrachromosomally thus minimizing the risks associated with insertional mutagenesis The virus is easily produced at high titers and is stable so that it can be purified and stored Even in the replication-competent form, adenoviruses cause only low level morbidity and are not associated with human malignancies Accordingly, adenovirus vectors have been developed which make use of these advantages For a description of adenovirus vectors and their uses see, e.g., Haj-Ahmad and Graham (1986) J Virol. 57 267-274, Bett et al. (1 93) J. Virol 67:5911-5921; Mittereder et al. (1994) Human Gene Therapy 5 717-729, Seth et al. ( 1994) J. Virol. 68:933-940; Barr et al. (1994) Gene Therapy 1:51-58; Berkner, K.L ( 1988) BioTechniques 6 616-629; Rich et al. (1993) Human Gene Therapy 4 461-476 Despite these advantages, adenovirus vectors suffer from several drawbacks. For example, adenovirus vectors express proteins transiently because the transferred gene does not integrate into the chromosome of the target cell. Hence, as the cells divide, the transferred gene is lost In this regard, such vectors are ineffective for long term gene therapy Furthermore, adenovirus vectors express viral proteins that may elicit an immune response which may decrease the life of the transduced cell. This immune response may preclude subsequent treatments because of humoral and/or T cell responses Still other attempts have been made to perfect gene delivery systems For example, adeno-associated virus (AAV) systems have also been developed. AAV is a human DNA parvovirus which belongs to the genus Dependovirus. The AAV genome is composed of a linear, single-stranded DNA molecule which contains approximately 4680 bases (Berns and Bohenzky (1987) Advances in Virus Research (Academic Press, Inc ) 12 243-307) The genome includes inverted terminal repeats (ITRs) at each end which function in cis as origins of DNA replication and as packaging signals for the virus The internal nonrepeated portion of the genome includes two large open reading frames, known as the AAV rep and cap regions, respectively These regions code for the viral proteins involved in replication and packaging of the viπon A family of at least four viral proteins are synthesized from the AAV rep region, Rep 78, Rep 68, Rep 52 and Rep 40, named according to their apparent molecular weight The AAV cap region encodes at least three proteins, VP1, VP2 and NP3 For a detailed description of the AAV genome, see, e g , Muzyczka, Ν (1992) Current Topics in Microhiol and Immunol \___ 97-129, Kotin, R M (1994) Human Gene Therapy 5 793- 801 , Berns, K I "Parvoviπdae and their Replication" in Fundamental Virology, 2nd Edition, (B Ν Fields and D M Knipe, eds ), pages 817-837 AAV requires coinfection with an unrelated helper virus, such as adenovirus, a herpesvirus or vaccinia, in order for a productive infection to occur In the absence of such coinfection, AAV establishes a latent state by insertion of its genome into a host cell chromosome Subsequent infection by a helper virus rescues the integrated copy which can then replicate to produce infectious viral progeny Unlike the retroviruses, AAV has a wide host range and is able to replicate in cells from any species so long as there is coinfection with a helper virus that will also multiply in that species Thus, for example, human AAV will replicate in canine cells coinfected with a canine adenovirus Furthermore, unlike the retroviruses, AAV is not associated with any human or animal disease, does not appear to alter the biological properties of the host cell upon integration and is able to integrate into nondivid g cells It has also recently been found that AAV is capable of site-specific integration into a host cell genome In light of the above-described properties, a number of recombinant AAV vectors have been developed for gene delivery See, e g , U S Patent Νos 5,173,414 and 5,139,941 , International Publication Νos WO 92/01070 (published 23 January 1992) and WO 93/03769 (published 4 March 1993), Lebkowski et al (1988) Mo/ec Cell Biol 8 3988-3996, Vincent et al (1990) Vaccines 90 (Cold Spring Harbor Laboratory Press), Carter, B J (1992) Current Opinion in Biotechnology 3_ 533-539, Muzyczka, Ν (1992) Current Topics in Microhiol and Immunol 158 97-129, Kotin, R M (1994) Human Gene Therapy 5 793-801, Shelling and Smith (1994) Gene Therapy \ 165-169, and Zhou et al (1994) J Exp Med 179 1867-1875 Recombinant AAV viπons can be produced in a suitable host cell which has been transfected with both an AAV helper plasmid and an AAV vector An AAV helper plasmid generally includes AAV rep and cap coding regions, but lacks AAV ITRs Accordingly, the helper plasmid can neither replicate nor package itself An AAV vector generally includes a selected gene of interest bounded by AAV ITRs which provide for viral replication and packaging functions Both the helper plasmid and the AAV vector bearing the selected gene are introduced into a suitable host cell by transient transfection The transfected cell is then infected with a helper virus, such as an adenovirus, which transactivates the AAV promoters present on the helper plasmid that direct the transcription and translation of AAV rep and cap regions Recombinant AAV viπons harboring the selected gene are formed and can be purified from the preparation However, integration, when it occurs, does not appear to be site specific Although such recombinant AAV viπons have proven useful for introducing several small gene sequences into host cells, gene delivery systems based on those particles are limited by the relatively small size of AAV particles More particularly, due to the size of the wild-type AAV genome, gene sequences of interest that are larger than about 5 Kb cannot be successfully packaged into AAV viπons This feature greatly reduces the range of gene delivery protocols that can be practiced using AAV viπons Based upon the discussion presented above, it should be evident that current gene delivery methods are suboptimal Accordingly, it would be useful to provide nucleotide sequence integration systems that are capable of the site-specific integration features provided by AAV viπons, yet are not limited in the size of the nucleotide sequence that can be integrated In this manner, large DNA molecules can be inserted into suitable target cells without the concomitant risk of insertional mutagenesis due to random integration events such as have been experienced with various prior systems Until the present invention, this unique set of features has not been provided in gene integration systems that will be practical for therapeutic use SUMMARY OF THE INVENTION The present invention provides for novel systems for gene integration In particular, AAV derived vector systems are described which allow for the integration of a selected nucleotide sequence into a recipient cell genome Integration can be targeted to a defined and benign genomic site, thereby eliminating the risk of insertional mutagenesis which can occur with viruses which integrate randomly The selected nucleotide sequence is not limited by size as in previous systems, since the AAV derived vector systems are not packaged in AAV viπons The vectors can be provided in recombinant molecules, including a plasmid or a virus. For example, the vectors can be provided in recombinant adenoviruses, thereby combining the advantages of prior adenovirus and AAV gene delivery systems while avoiding their limitations. The system has a broad host range and can be used to infect quiescent as well as replicating target cells. Recombinant viruses can be produced at high titers and are stable so that they can be purified and stored. Furthermore, the system is safe and does not cause human diseases or cancers. Accordingly, in one embodiment, the invention is directed to a method of integrating a selected nucleotide sequence into the genome of a mammalian cell, comprisin (a) providing: (i) a first nucleic acid construct comprising a nucleotide sequence flanked by a 5' and a 3' adeno-associated virus inverted terminal repeat, and (ii) a second nucleic acid construct having a rep coding region operably linked to control elements capable of directing the transcription and translation of the rep coding region in the mammalian cell; (b) expressing the rep coding region of the second nucleic acid construct, thereby producing an amount of rep expression product capable of facilitating the integration of the nucleotide sequence of the first nucleic acid construct; and (c) integrating the nucleotide sequence of the first nucleic acid construct into the mammalian cell, the integration being facilitated by the rep expression product. In a further embodiment, the invention contemplates a mammalian cell containing the same nucleic acid constructs. In another embodiment, the invention contemplates a method of integrating a selected nucleotide sequence into the genome of a mammalian cell, comprising: (a) providing: (i) a first nucleic acid construct comprising a nucleotide sequence flanked by a 5' and a 3 1 adeno- associated virus inverted terminal repeat, and (ii) an amount of rep expression product capable of facilitating the integration of the nucleotide sequence of the first nucleic acid construct; and (b) integrating the nucleotide sequence of the first nucleic acid construct into the mammalian cell, the integration being facilitated by the rep expression product. Moreover, a further embodiment of the present invention contemplates a mammalian cell containing the same nucleic acid constructs. In some embodiments, the first nucleic acid construct is a recombinant adeno- associated virus. In further embodiments, the first nucleic acid construct is a plasmid; the mammalian cell is transfected with the plasmid in particular embodiments. In particular embodiments, the first nucleic acid construct and the second nucleic acid construct are present on the same vector. Conversely, the first nucleic acid construct and the second nucleic acid construct are present on different vectors in other embodiments. Moreover, the invention contemplates a nucleotide sequence of the first nucleic acid construct that is integrated into a chromosome of the mammalian cell In particular embodiments, the chromosome is chromosome 19q. In some embodiments, the nucleotide sequence of the first nucleic acid construct is more than 5,000 base pairs, while it is more than 10,000 base pairs in other embodiments. In further embodiments, the nucleotide sequence of the first nucleic acid construct encodes a polypeptide. The polypeptide encodes at least a portion of the factor VIII gene in some embodiments. That is, the present invention is not limited to a particular sequence encoding a protein that retains factor VIII activity. The invention is directed to recombinant DNA molecules, and preferably to plasmids, comprising the nucleic acid constructs disclosed above. In some embodiments, the invention is directed to a recombinant virus comprising the above-described nucleic acid constructs, as well as to target cells infected with the recombinant virus. In one particular embodiments, the virus is a recombinant adenovirus. In yet additional embodiments, the invention is directed to a method of treating an acquired or inherited disease in a subject, comprising transfecting or infecting a selected target cell from the subject with a plasmid or recombinant virus, respectively comprising the nucleic acid constructs as described above. These and other embodiments of the subject invention will readily occur to those of ordinary skill in the art in view of the disclosure herein. BRIEF DESCRIPTION OF THE FIGURES Figure 1 is a depiction of one embodiment of the subject invention wherein a recombinant adenovirus which includes a nucleic acid construct comprising a rep coding region and a selected nucleotide sequence bounded by AAV ITRs, is used to infect a target cell. The presence of both the rep protein and the AAV ITRs serve to cause targeted integration of the selected nucleotide sequence into chromosome 19 of the target cell Figure 2 diagramatically depicts several strategies used in the examples to demonstrate rep mediated integration of the selected nucleotide sequence into chromosome 19 of the target cell Figure 3 depicts several diagrams of nucleic acid constructs of pAAV and derivatives thereof. Figure 4 is a diagram of the AAVSl region of chromosome 19 depicting an AAV derivative {i.e., a provirus) integrated into the AAVS l region. Each inverted viral terminal repeat is symbolized by an open rectangle containing a solid triangle; an expanded view of one of the ITRs is also shown. The numbered solid arrows represent primers. Figure 5A indicates whether site specific integration occurred in 293 cells infected with wild type AAV2 at three different multiplicities of infection. Figure 5B indicates whether site specific integration occurred in HeLa cells infected with wild type AAV2 at three different multiplicities of infection. Figure 6 indicates whether site specific integration occurred in 293 cells transfected with a recombinant AAV vector plasmid or derivatives thereof. Figure 7A depicts diagrams of targeting vectors used in the experiments described in the examples Figure 7B depicts diagrams of helper plasmids used in the experiments described in the examples. Figure 8 is a diagram illustrating steps used in the construction of p2TRcos. Figure 9 indicates whether site specific integration occurred in 293 cells transfected alone with either p2TRlacZ, p2TRF8, or p2TRcos; cotransfected with either p2TRlacZ and pRR5, p2TRF8 and pRR5, or p2TRcos and pRR5; and cotransfected with either p2TRlacZ and pGN1764, p2TRF8 and pGN1764, or p2TRcos and pGN1764. Figure 10 depicts the results of a Southern Blot performed on β-galactosidase-positive clones of 293 cells transfected with a targeting vector and a helper plasmid. Figure 1 1 diagramatically illustrates the fluorescence in situ hybridization (FISH) strategy used in the examples. Figure 12A diagrammatically depicts some of the characteristics of the AAVSl region of chromosome 19. Figure 12B diagrammatically depicts some of the characteristics of p2TRlacZ that was co-transfected with helper plasmid pRR5 (not shown) into 293 cells to produce a β- galactosidase-positive clone manipulated in some of the experiments. Figure 12C diagrammatically depicts, in the top portion, arrangement of the integrated p2TRlacZ plasmid sequences within chromosome 19. Sequence information pertaining to the left junction of the integration site is shown in the bottom left portion, while sequence O 96/18727 PCΪ7US95/16413 information pertaining to the right |unctιon of the integration site is shown in the bottom right portion DETAILED DESCRIPTION OF THE INVENTION The practice of the present invention will employ, unless otherwise indicated, conventional methods of virology, microbiology, molecular biology and recombinant DNA techniques within the skill of the art Such techniques are explained fully in the literature See, e g , Sambrook, et al Molecular Cloning A Laboratory Manual (Current Edition), DNA Cloning A Practical Approach, vol I & II (D Glover, ed ), Ohgonucleotide Synthesis (N Gait, ed , Current Edition), Nucleic Acid Hybridization (B Hames & S Higgms, eds , Current Edition), Transcription and Translation (B Hames & S Higgins, eds , Current Edition), CRC Handbook of Pan oviruses, vol I & II (P Tiiessen, ed ), Fundamental Virology, 2nd Edition, vol I & II (B N Fields and D M Knipe, eds ) All publications, patents and patent applications cited herein, whether supra or mfia, are hereby incorporated by reference in their entirety As used in this specification and the appended claims, the singular forms "a," "an" and "the" include plural references unless the content clearly dictates otherwise A. Definitions In describing the present invention, the following terms will be employed, and are intended to be defined as indicated below "Gene transfer" or "gene delivery" refers to methods or systems for reliably inserting a particular nucleotide sequence (e g , DNA) into targeted cells Such methods preferably result in the integration of the transferred genetic material into the genome of target cells Gene transfer provides a unique approach for the treatment of acquired and inherited diseases, and a number of systems have been developed in the art for gene transfer into mammalian cells See, e g , \J S> Patent No 5,399,346 By "adeno-associated virus inverted terminal repeats" or "AAV ITRs" is meant the art- recognized pa ndromic regions found at each end of the AAV genome which function together in cis as origins of DNA replication and as packaging signals for the virus For use with the present invention, flanking AAV ITRs are positioned 5' and 3' of one or more selected heterologous nucleotide sequences and, together with the rep coding region or the Rep expression product, provide for the integration of the selected sequences into the genome of a target cell The nucleotide sequences of AAV ITR regions are known See, e g , Kotin, R M (1994) Human Gene Therapy 5 793-801, Berns, K I "Parvoviπdae and their Replication" in Fundamental Virology, 2nd Edition, (B N Fields and D M Knφe, eds ) for the AAV-2 sequence As used herein, an "AAV ITR" need not have the wild-type nucleotide sequence depicted, but may be altered, e g , by the insertion, deletion or substitution of nucleotides Additionally, the AAV ITR may be derived from any of several AAV serotypes, including without limitation, AAV- 1, AAV-2, AAV-3, AAV-4, AAV-5, AAVX7, etc The 5' and 3 ' ITRs which flank a selected heterologous nucleotide sequence need not necessarily be identical or derived from the same AAV serotype or isolate, so long as they function as intended, I e , to allow for the integration of the associated heterologous sequence into the target cell genome when the rep gene is present (either on the same or on a different vector), or when the Rep expression product is present in the target cell By "vector" is meant any genetic element, such as a plasmid, phage, transposon, cosmid, chromosome, virus, viπon, etc , which is capable of replication when associated with the proper control elements and which can transfer gene sequences between cells Thus, the term includes cloning and expression vehicles, as well as viral vectors By an "AAV vector" is meant a vector derived from an adeno-associated virus serotype, including without limitation, AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAVX7, etc AAV vectors can have one or more of the AAV wild-type genes deleted in whole or part, preferably the rep and/or cap genes, but retain functional flanking ITR sequences AAV vectors can be constructed using recombinant techniques that are known in the art to include one or more heterologous nucleotide sequences flanked on both ends (5 and 3 ' ) with functional AAV ITRs In the practice of the invention, an AAV vector can include at least one AAV ITR and a suitable promoter sequence positioned upstream of the heterologous nucleotide sequence and at least one AAV ITR positioned downstream of the heterologous sequence A "recombinant AAV vector plasmid" refers to one type of recombinant AAV vector wherein the vector comprises a plasmid As with AAV vectors in general, 5' and 3' ITRs flank the selected heterologous nucleotide sequence AAV vectors can also include transcription sequences such as polyadenylation sites, as well as selectable markers or reporter genes, enhancer sequences, and other control elements which allow for the induction of transcription Such control elements are described more fully below As used herein, the term "AAV virion" refers to a complete virus particle An AAV virion may be a wild type AAV particle (comprising a linear, single-stranded AAV nucleic acid genome associated with an AAV capsid, i.e., a protein coat), or a recombinant AAV particle (described below) In this regard, single-stranded AAV nucleic acid molecules (either the sense/coding strand or the antisense/anticoding strand as those terms are generally defined) can be packaged into an AAV virion, both the sense and the antisense strands are equally infectious As used herein, the term "recombinant AAV virion" or "rAAV" is defined as an infectious, replication-defective virus composed of an AAV protein shell encapsidating (i.e., surrounding with a protein coat) a heterologous nucleotide sequence, which in turn is flanked 5' and 3' by AAV ITRs A number of techniques for constructing recombinant AAV virions are known in the art See, e.g., U S Patent No. 5, 173,414, International Publication Numbers WO 92/01070 (published 23 January 1992) and WO 93/03769 (published 4 March 1993), Lebkowski et al. (1988) Molec. Cell. Biol. 8.3988-3996; Vincent et al. (1990) Vaccines 90 (Cold Spring Harbor Laboratory Press); Carter, B.J (1992) Current Opinion in Biotechnology 3 533-539, Muzyczka, N (1992) Current Topics in Microhiol. and Immunol. 158 97-129. Kotin, R M (1994) Human Gene Therapy 5:793-801 ; Shelling and Smith ( 1994) Gene Therapy 1 165-169, and Zhou et al. (1994) J. Exp. Med. 179.1867-1875 Suitable nucleotide sequences for use in AAV vectors include any functionally relevant nucleotide sequence Thus, the AAV vectors of the present invention can comprise any desired gene that encodes a protein that is defective or missing from a target cell genome or that encodes a non-native protein having a desired biological or therapeutic effect (e , an antiviral function), or the sequence can correspond to a molecule having an antisense or ribozyme function. Suitable genes include those used for the treatment of inflammatory diseases, autoimmune, chronic and infectious diseases, including such disorders as AIDS, cancer, neurological diseases, cardiovascular disease, hypercholestemia; various blood disorders including various anemias, thalasemias and hemophilia; genetic defects such as cystic fibrosis, Gaucher's Disease, adenosine deaminase (ADA) deficiency, emphysema, etc A number of antisense oligonucleotides (e.g., short oligonucleotides complementary to sequences around the translational initiation site (AUG codon) of an mRNA) that are useful in antisense therapy for cancer and for viral diseases have been described in the art See, e.g , Han et al (1991 ) Proc Nat/ Acad Set USA 88 4313-4317, Uhlmann et at (1990) Chem Rev 90 543-584, Helene et al (1990) Biochim Biophvs Ada 1049 99-125, Agarwal el al (1988) Proc Nail Acad Sci USA 85 7079-7083, and Heikkila et al (1987) Nature 328 445- 449 For a discussion of suitable πbozymes, see, e g , Cech et al ( 1992) / Biol Chem 267 17479-17482 and U S Patent No 5,225,347 to Goldberg et al By "recombinant virus" is meant a virus that has been genetically altered, e g , by the addition or insertion of a heterologous nucleic acid construct into the particle As used herein, the terms "facilitate," "facilitation," and the like refer broadly to making an action or result more easily achieved For example, the expression product of the rep gene is required to target selected nucleotide sequences into a cell's genome The term includes, but is not limited to, site specific integration of nucleotide sequences into chromosome 19 By " AAV rep coding region" is meant the art-recognized region of the AAV genome which encodes the replication proteins of the virus which are collectively required for replicating the viral genome, or functional homologues thereof such as the human herpesvirus 6 (HHV-6) rep gene which is also known to mediate AAV-2 DNA replication (Thomson et al (1994) Virology 204 304-31 1) Thus, the rep coding region includes at least the genes encoding for AAV Rep 78 and Rep 68 (the "long forms of Rep"), and Rep 52 and Rep 40 (the "short forms of Rep"), or functional homologues thereof For a further description of the AAV rep coding region, see, e g , Muzyczka, N (1992) Current Topics m Microhiol and Immunol \___ 97-129, and Kotin, R M (1994) Human Gene Therapy 5 793-801 The rep coding region, as used herein, can be derived from any viral serotype, such as the AAV serotypes described above The region need not include all of the wild-type genes but may be altered, e g , by the insertion, deletion or substitution of nucleotides, so long as the rep genes present provide for sufficient integration functions when expressed in a suitable target cell "Rep expression products" are defined herein to include both the short forms and the long forms of AAV Rep, including functional homologous thereof The term "transfection" is used to refer to the uptake of foreign DNA by a cell and a cell has been "transfected" when exogenous DNA has been introduced inside the cell membrane A number of transfection techniques are generally known in the art See, e g , Graham et al (1973) Virology, 52 456, Sambrook et al (1989) Molecular Cloning, a laboratory manual, Cold Spring Harbor Laboratories, New York, Davis et al ( 1986) Basic Methods in Molecular Biology, Elsevier, and Chu et al (1981) Gene J_3 197 Such techniques can be used to introduce one or more exogenous DNA moieties, such as an AAV vector, AAV helper constructs, and other nucleic acid molecules, into suitable host cells As used herein, a "nucleotide sequence integration system" intends the operative combination of (1 ) a first nucleic acid construct comprising a nucleotide sequence of interest flanked by a 5' and a 3' AAV ITR, and (2) either a further nucleic acid construct having a rep coding region operably linked to control elements capable of directing the transcription and translation of the rep coding region in a suitable target cell, or a suitable amount of Rep expression products, whereby the nucleotide integration system provides for the integration of the nucleotide sequence of interest from the first construct into the genome of a target cell that has been transfected with said integration system By "target cell," or "target mammalian cell" is meant a cell which has been transformed, or is capable of transformation, by a nucleic acid construct or an AAV vector bearing a selected nucleotide sequence of interest The term includes the progeny of the parent cell, whether or not the progeny is identical in morphology or in genetic make-up to the original parent, so long as the selected nucleotide sequence is present A cell has been "stably transformed" with a nucleic acid construct comprising a selected nucleotide sequence of interest when the construct has been introduced inside the cell membrane and the sequence of interest has been integrated into the target cell genome such that the integrated nucleotide sequence is capable of being inherited by daughter cells through chromosome replication The cell can be transformed using any of several techniques, including transduction, transfection and infection Stability is demonstrated by the ability of the target cell to establish cell lines or clones comprised of a population of daughter cells which contain the nucleotide sequence of interest The term "heterologous" as it relates to nucleic acid sequences such as coding sequences and control sequences, denotes sequences that are not normally joined together, and/or are not normally associated with a particular cell Thus, a "heterologous" region of a nucleic acid construct or an AAV vector is a segment of nucleic acid within or attached to another nucleic acid molecule that is not found in association with the other molecule in nature For example, a heterologous region of a nucleic acid construct could include a coding sequence flanked by sequences not found in association with the coding sequence in nature Another example of a heterologous coding sequence is a construct where the coding sequence itself is not found in nature (e g , synthetic sequences having codons different from the native gene) Similarly, a cell transformed with a construct which is not normally present in the cell would be considered heterologous for purposes of this invention. Allelic variation or naturally occurring mutational events do not give rise to heterologous DNA, as used herein. A "coding sequence" or a sequence which "encodes" a particular protein, is a nucleic acid sequence which is transcribed (in the case of DNA) and translated (in the case of mRNA) into a polypeptide in vitro or in vivo when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence are determined by a start codon at the 5' (amino) terminus and a translation stop codon at the 3 1 (carboxy) terminus. A coding sequence can include, but is not limited to, cDNA from procaryotic or eucaryotic mRNA, genomic DNA sequences from procaryotic or eucaryotic DNA, and even synthetic DNA sequences. A transcription termination sequence will usually be located 3' to the coding sequence. A "nucleic acid" sequence refers to a DNA or RNA sequence. The term captures sequences that include any of the known base analogs of DNA and RNA such as, but not limited to 4-acetylcytosine, 8-hydroxy-N6-methyladenosine, aziridinylcytosine, pseudoisocytosine, 5-(carboxyhydroxylmethyl) uracil, 5-fluorouracil, 5 >romouracil, 5- carboxymethylaminomethyl-2-thiouracil, 5-carboxymethylaminomethyluracil, dihydrouracil, inosine, N6-isopentenyladenine, 1-methyladenine, 1-methylpseudouracil, 1-methylguanine, 1- methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5- methylcytosine, N6-methyladenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxy- aminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5'-methoxycarbonylmethyluracil, 5- methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, oxybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2- thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, N-uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, pseudouracil, queosine, 2-thiocytosine, and 2,6-diaminopurine. The terms DNA "control sequences" and "control elements" refer collectively to promoter sequences, polyadenylation signals, transcription termination sequences, upstream regulatory domains, origins of replication, internal ribosome entry sites ("IRES"), enhancers, and the like, which collectively provide for the replication, transcription and translation of a coding sequence in a recipient cell. Not all of these control sequences/elements need always be present so long as the selected coding sequence is capable of being replicated, transcribed and translated in an appropriate host cell. "Operably linked" refers to an arrangement of elements wherein the components so described are configured so as to perform their usual function. Thus, control sequences - ι: operably linked to a coding sequence are capable of effecting the expression of the coding sequence The control sequences need not be contiguous with the coding sequence, so long as they function to direct the expression thereof Thus, for example, intervening untranslated yet transcribed sequences can be present between a promoter sequence and the coding sequence and the promoter sequence can still be considered "operably linked" to the coding sequence "Homology" refers to the percent of identity between two polynucleotide or two polypeptide moieties The correspondence between the sequence from one moiety to another can be determined by techniques known in the art For example, homology can be determined by a direct comparison of the sequence information between two polypeptide molecules by aligning the sequence information and using readily available computer programs Alternatively, homology can be determined by hybridization of polynucleotides under conditions which form stable duplexes between homologous regions, followed by digestion with single-stranded-specific nuclease(s), and size determination of the digested fragments Two DNA, or two polypeptide sequences are "substantially homologous" to each other when at least about 80%, preferably at least about 90%, and most preferably at least about 95% of the nucleotides or amino acids match over a defined length of the molecules, as determined using the methods above A "functional homologue," or a "functional equivalent" of a given polypeptide includes molecules derived from the native polypeptide sequence, as well as recombinantly produced or chemically synthesized polypeptides which function in a manner similar to the reference molecule to achieve a desired result Thus, a functional homologue of AAV Rep expression products (e g , Rep 78, Rep 68, Rep 52 and Rep 40) encompasses derivatives and analogues of those polypeptides—including any single or multiple amino acid additions, substitutions and/or deletions occurring internally or at the ammo or carboxy termini thereof, so long as replication or integration activity remains B. General Methods Central to the present invention is the development of a nucleotide sequence integration system which allows for the efficient targeting and integration of a selected nucleotide sequence into the genome of a target mammalian cell A particular feature of the invention is that such integration can be directed to non-essential regions of particular chromosomes of the target cell, thereby avoiding the risk of insertional mutagenesis which can occur with random integration of a viral vector More particularly, the present invention makes use of a DNA vector comprising a first nucleic acid construct bearing a selected nucleotide sequence The selected sequence is flanked on the 5' and 3' ends with AAV ITRs Also present, on either the same or a different vector, is a second nucleic acid construct which includes a rep coding region Furthermore, when present on the same vector, the rep coding region can be positioned either upstream or downstream of the selected nucleotide sequence A particular embodiment of the subject invention, wherein the rep coding region and the selected nucleotide sequence are found on the same DNA vector, is shown in Figure 1. The first and second nucleic acid constructs can be engineered using recombinant techniques known to those of skill in the art. In particular, the first nucleic acid construct which harbors the nucleotide sequence of interest bounded by the AAV ITRs, can be constructed by directly inserting a selected sequence into an AAV genome which has the rep and cap coding regions excised, e g using restriction enzymes Other portions of the AAV genome can also be deleted, so long as a sufficient portion of the ITRs remain to allow for integration functions Such constructs can be designed using techniques well known in the art. See, e.g., U.S. Patent Nos. 5,173,414 and 5,139,941 ; International Publication Nos WO 92/01070 (published 23 January 1992) and WO 93/03769 (published 4 March 1993), Lebkowski et al. (1988) Molec. Cell. Biol. 8.3988-3996; Vincent et al. (1990) Vaccines 90 (Cold Spring Harbor Laboratory Press), Carter, B.J. (1992) Current Opinion in Biotechnology 3:533-539, Muzyczka, N. ( 1992) Current Topics in Microhiol. and Immunol. 158 97-129. Kotin, R.M. (1994) Human Gene Therapy 5:793-801; Shelling and Smith (1994) Gene Therapy 1. 165-169; and Zhou et al. (1994) J. Exp. Med. 179: 1867-1875 Alternatively, the AAV ITRs can be excised from the viral genome or from an AAV vector containing the same and fused 5' and 3' of the selected nucleotide sequence which is present in another vector, using standard ligation techniques, such as those described in Sambrook et al, supra For example, ligations can be accomplished in 20 mM Tris-Cl pH 7.5, 10 mM MgCl-, 10 mM DTT, 33 ug/ml BSA, 10 mM-50 mM NaCl, and either 40 uM ATP, 0.01-0 02 (Weiss) units T4 DNA ligase at 0°C (for "sticky end" ligation) or 1 M ATP, 0.3-0 6 (weiss) units T4 DNA ligase at 14°C (for "blunt end" ligation) Intermolecular "sticky end" ligations are usually performed at 30-100 μg/ml total DNA concentrations (5- 100 nM total end concentration). AAV vectors which contain ITRs have been described in, e.g., U.S Patent no. 5, 139,941. In particular, several AAV vectors are described therein which are available from the American Type Culture Collection ("ATCC") under accession numbers 53222, 53223, 53224, 53225 and 53226 Additionally, chimeπc genes can be produced synthetically which include the AAV ITR sequences fused 5' and 3' of the selected nucleotide sequence AAV ITR nucleotide sequences are known See, e g , Kotin, R M (1994) Human Gene Therapy 5 793-801 , and Berns, K I "Parvoviπdae and their Replication" in Fundamental Virology, 2nd Edition, (B N Fields and D M Knipe, eds ), for the AAV-2 sequence Preferred codons for expression of the chimeπc nucleotide sequence in mammalian cells can be used The complete chimeπc sequence is assembled from overlapping oligonucleotides prepared by standard methods See, e g . Edge, Nature ( 1981) 292 756, Nambair et al Science (1984) 223 1299, Jay et al I Biol Chem ( 1984) 259 631 1 The selected nucleotide sequence of the first nucleic acid construct can comprise any desired gene that encodes a protein that is defective or missing from a recipient cell genome or that encodes a non-native protein having a desired biological or therapeutic effect (e g , an antiviral function), or the sequence can correspond to a molecule having an antisense or πbozyme function Suitable genes include those used for the treatment of inflammatory diseases, autoimmune, chronic and infectious diseases, including such disorders as AIDS cancer, neurological diseases, cardiovascular disease, hypercholestemia, various blood disorders including various anemias, thalasemias and hemophilia, genetic defects such as cystic fibrosis, Gaucher's Disease, adenosine deaminase (ADA) deficiency, emphysema, etc As explained above, a second nucleic acid construct is also provided which includes a rep coding region The presence of rep provides for targeted insertion of the selected nucleotide sequence (from the first nucleic acid construct) into non-essential regions of particular chromosomes More particularly, Rep expression products are thought to mediate integration events which take place between compatible AAV ITR sequences and sequences present in a target cell genome In this regard, it has been shown that the integration locus for AAV (termed "AAVSl ") is human chromosome 19ql3 3-qter European Patent Appln No 931 14941 3 (Publication No 0 592 836 Al), Samulski et al (1991) EMBO K) 3941- 3950, Kotin et al (1992) EMBO J U 5071-5078 AAV vectors including the AAV rep coding region positioned between the ITRs have been shown to integrate into AAVSl of chromosome 19 Shelling and Smith ( 1994) Gene Therapy __ 165-169 Rep recognition sequences have also been identified on human chromosome 19 near sites of viral integration in AAVSl Weitzman et al (1994) Proc Nail Acad Sa USA 9J_ 5808-5812 AAV constructs have also been shown to integrate into chromosome 17 Walz and Schlehofer (1992) J Vnol 66 2990-3002 The rep coding region can be obtained from the viral genome or from a vector known to include the same In this regard, a number of rep containing AAV vectors are known, including the several vectors described in, e g , U S Patent no 5, 139,941 , having ATCC accession numbers 53222, 53223, 53224, 53225 and 53226 Similarly, methods of obtaining the HHV-6 homologue of AAV rep are described in Thomson el al (1994) Virology 204 304- 31 1 The rep coding region will be operably linked to control sequences that direct the transcription and translation thereof Such control elements include one or more of promoters, polyadenylation signals, transcription termination sequences, upstream regulatory domains, replication sequences, enhancers, and the like, which collectively provide for the transcription and translation of the rep coding region when present in the target cell Useful promoter sequences include those derived from sequences encoding mammalian viral genes Examples include but are not limited to the homologous AAV promoters, the SV40 early promoter, mouse mammary tumor virus LTR promoter, adenovirus maior late promoter (Ad MLP), a herpes simplex virus (HSV) promoter, a cytomegalovirus (CMV) promoter, a rous sarcoma virus (RSV) promoter, synthetic promoters, hybrid promoters, and the like In addition, sequences derived from nonviral genes, such as the muπne metallothionein gene, will also find use herein Such promoter sequences are commercially available from, e g , Stratagene Additionally, regulatory elements can be picked that allow for the controlled expression of the rep coding region in the target cell Such elements are turned on in response to an appropriate effector In this way, the Rep proteins can be made when integration of the desired nucleotide sequence into the genome of the target cell is desired Regulatory sequences are known to those of skill in the art, and include e g , elements derived from the lac operator-repressor system (see, e g , Hu and Davidson Cell (1987) 48 555-566), origins of replication including those derived from papovaviruses, such as the SV40 origin of replication (SV40oπ) for which the T antigen is the effector, as well as cellular origins of replication, such as the dihydrofolate reductase (dhfr) gene for which methotrexate is the effector See, e g Urlaub et al (1980) Proc Natl Acad Sci USA 77 4216-4220, Rungold et al (1981) ./ Mol and Appl Genet 1 165-175 For these methods, the appropriate effector will be available in the target cell at the time that expression of the AAV tep coding region is desired Systems for administering regulatory compounds are known in the art See, e g , International Publication No WO 88/09809, McVey et al (1989) Mol Cell Biol 9 5525-5536, and Van Doren et al (1984) Mol Cell Biol 9 5525-5536 The second nucleic acid construct which includes the AAV rep coding region may also include selectable markers, such as genes which confer antibiotic resistance or sensitivity, or impart color, or change the antigenic characteristics, when the transformed cells are grown in an appropriate selective medium Once engineered, the constructs can be used directly to transform a selected target cell In this regard, the cell to be transformed will depend on the purpose for gene transfer, e g , the disease state being treated For example, the system of the present invention can be used to deliver and integrate nucleotide sequences into any nucleated cell including stem, progenitor and erythroid cells, as well as any of the various white blood cells such as lymphocytes, neutrophils, eosinophils, basophils, monocytes, tissue specific cells, such as those derived from lung, heart, kidney, liver, spleen, pancreatic tissue, connective tissue, muscle and bone tissue including osteocytes, gang ocytes, epithelial and endothelial cells, ependymal cells, reticuloendothelial cells, dendritic and neural cells, and the like Generally, target cells will be transformed with the nucleotide sequence integration system of the present invention either /// vivo or ex vivo If transformed ex vivo, the desired target cell type will be removed from the subject, transformed and reintroduced into the subject In this regard, a number of methods are known in the art for transforming cells, including dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the nucleic acid constructs in Iiposomes, and direct microinjection of the DNA into nuclei Such systems are known in the art and have been described in e g , Finney and Bishop (1993) Science 260 1524-1527 The transformed cells can be screened for those cells harboring the selected gene, using conventional techniques such as Southern blots and/or PCR If delivered in vivo, the nucleic acid constructs will be formulated into pharmaceutical compositions and will generally be administered parenterally, e g , by injection Additional formulations suitable for other modes of administration include oral and pulmonary formulations, suppositories, and transdermal applications Dosage treatment may be a single dose schedule or a multiple dose schedule One of skill in the art can readily determine an appropriate dosage using standard dose response curves Alternatively, recombinant viruses can be used to deliver the constructs of the present invention to the target cell. In particular, the present invention has been exemplified using an adenovirus as the delivery system, as depicted in Figure 1 However, any recombinant virus which is capable of infecting the target cell of interest, will find use in the present system In this regard the nucleic acid constructs will first be used to transform a selected virus and the transformed virus, in turn, used to deliver a selected nucleotide sequence to a suitable target cell If adenoviruses are used, any of the various human adenovirus strains, such as but not limited to, adenovirus type 2 (Ad2), adenovirus type 5 (Ad5), adenovirus type 7 (Ad7), adenovirus type 12 (Ad 12), will find use herein Other adenoviruses which will also be useful with the present invention include mutant adenoviruses which have been manipulated, such as by the removal of nonessential regions of the genome, to allow for packaging of larger quantities of foreign DNA See, e.g., Haj-ahmad and Graham ( 1986) J. Virol. ^7 267- 274 which describes adenovirus dlE\,3, an Ad5 virus which has most of the early region 3 (E3) and early region 1 (El) deleted and therefore accepts up to about 7 5 kb of foreign DNA, Bett et al. (1993) ./ Virol. 67:591 1-5921, which discloses adenovirus mutants with 1.88 and 3 00 kb E3 deletions, Trapnell, B C (1993) Advanced Drug Delivery Revie vs \2 185- 199, describing Aά-dI327, an Ad5-derived mutant with a deletion of Xbάl fragment D Additionally, adenovirus chimeric vectors, such as those described in Michael et al. J Biol. Chem. ( 1993) 268:6866-6869 and Wagner et al. Proc. Natl. Acad. Sci. USA (1992) 89 6099- 6103, can also be used for gene delivery Alternatively, a recombinant AAV virion can be used to deliver constructs of the present invention into the target cell. Recombinant AAV virions, as defined above, comprise a heterologous nucleotide sequence flanked 5' and 3' by AAV ITRs. Other carrier viruses which will find use for the delivery of the various nucleic acid constructs to the target cell include those derived from the pox family of viruses, including vaccinia virus and avian poxvirus, as well as any of the various herpesviruses such as HSV. CMV, EBV, VZ etc. The use of these viruses to deliver gene sequences is well known in the art Methods for the insertion of foreign genes in vaccinia virus have been described in detail See, e.g., Mackett, M et al. in DNA Cloning: A Practical Approach, vol II (D Glover, ed.) pp. 191-21 1; Mackett et al. (1984) J. Virol. 49:857-864, Fuerst et al. (1986) Proc. Nat/. Acad. Sci. USA 83 8122-8126; and U.S. Patent No 4,722,848 Alternatively, Avipoxviruses, such as the fowlpox and canarypox viruses, can also be used to deliver the constructs of the present invention Methods for producing recombinant Avipoxviruses are known in the art and employ genetic recombination, as described above with respect to the production of vaccinia viruses See, e.g., WO 91/12882, WO 89/03429, and WO 92/03545 The nucleic acid constructs of the present nucleotide sequence integration system can be used to deliver selected sequences to a variety of cell and tissue types for the production of transgenic organisms, as well as for gene therapy, vaccination, or for characterizing a variety of genes and the mechanism of their actions The instant methods will also find use in ribozyme and antisense therapy. For a review of antisense therapy and oligonucleotides useful in the same, see, Uhlmann, E and Peyman, A (1990) Chem. Rev. 90 543-584 For a discussion of ribozymes see, Cech et al. (1992) J. Biol. Chem. 267 17479-17482 As presented above, site-specific integration of large pieces of DNA into the human genome is a desirable tool both for research purposes and for practical gene therapy applications. Site-directed recombination represents an efficient way of achieving site-specific integration Currently, several site-directed recombination systems exist For example, the yeast flip recombinase (FLP) can mediate recombination between two "flip recombination target sites" (FRT) - one present on a plasmid and one present in a specific location in the chromosomal DNA O'Gorman et al., (1991) Science 251 : 1351-55 Similar results have been reported using the loxP-Cre recombination system, van Deursen et al. (1995) Proc. Nat/. Acad. Sci. USA 92 7376-80. Unfortunately, in both of these systems, the recombination site is not normally present in the human genome; instead, the site needs to be introduced, a very impractical process To overcome the problem of recombination systems presented in the preceding paragraph, a new system has been developed that is capable of mediating efficient site-specific integration of large pieces of DNA into a site normally present in all human cells This adeno-associated virus-based system is termed "Targeted Vector Integration" (TVI) As previously alluded to, AAV requires the presence of a helper virus for efficient replication In the absence of a helper virus coinfection, AAV integrates with high efficiency into human chromosome 19 (at position 19q 13 3-qter) As described above, AAV contains a large open reading rrame, AAV rep, that encodes at least four proteins implicated in the replication of the virus. The four proteins - Rep 78, Rep 68, Rep 52 and Rep 40 - are named according to their apparent molecular weight. For a detailed description of the AAV genome, see, e.g., Muzyczka, N. (1 92) Current Topics in Microhiol. and Immunol. 158:97-129; Kotin, R.M. (1994) Human Gene Therapy 5:793-801 , Berns, K.I. "Parvoviridae and their Replication" in Fundamental Virology, 2nd Edition, (B.N. Fields and D M. Knipe, eds.), pages 817-837. Rep 78 and Rep 68, termed the "long forms of Rep", are expressed from the p5 promotor (see below). The long forms of Rep possess a number of biochemical activities that directly implicates them in the replication of the virus. These activities include binding the viral inverted terminal repeats (ITRs), nicking at the terminal resolution site, and helicase activity. Kotin, R.M. (1994) Human Gene Therapy 5_:793-801. In addition, it has been shown that Rep78 and Rep 68 mediate the formation of a complex between the ITR in the hairpin configuration and a 109 bp sequence from the AAV integration site that contains a similar 12 nucleotide core rep binding site. Kotin, R.M. (1994) Human Gene Therapy 5 : 793-801. As previously alluded to, the present invention contemplates, among other things, the provision of a desired gene that encodes a protein that is defective or missing from a target cell genome in a patient. The present invention also contemplates a method of treating a patient suffering from a disease state by providing the patient with human cells genetically engineered to encode the required protein. In one embodiment, the cells may be genetically engineered in vitro by removing the cells from the patient, introducing the desired gene into the cells, then reintroducing the genetically engineered cells back into the patient. Of course, other methods of introducing desired genes into a patient are known and are within the scope of the present invention. For example, U.S. Patent No. 5,399,346 to Anderson et al., which is hereby incorporated by reference, discusses several other methods that may be used in conjunction with the present invention. C. Experimental Below are examples of specific embodiments for carrying out the present invention The examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way Efforts have been made to ensure accuracy with respect to numbers used (e.g. , amounts, temperatures, etc ), but some experimental error and deviation should, of course, be allowed for In the disclosure which follows, the following abbreviations apply °C (degrees Centigrade), H 2 O (water); HC1 (hydrochloric acid), MgSO 4 (magnesium sulfate), MgCl : (magnesium chloride), aa (amino acid); PCR (polymerase chain reaction), kd or kD (kilodaltons), nt (nucleotides), gm (grams); μg (micrograms), mg (milligrams), ng (nanograms), μL (microliters), mL (milliliters); mm (millimeters), nm (nanometers), μm (micrometer); M (molar), mM (millimolar); MW (molecular weight), sec (seconds), min(s) (minute/minutes), hr(s) (hour/hours), ATCC (American Type Culture Collection, Rockville, MD), DMEM (Dulbecco's Modification of Eagle's Medium), FBS (fetal bovine serum), dNTPs (deoxynucleotide triphosphates); BIO 101, Inc (La Jolla, CA), BioRad (BioRad, Richmond, CA), Boehringer (Boehringer Mannheim Corp, Indianapolis, IN), Genome Systems (Genome Systems, Inc., St. Louis, MO), New England Biolabs (New England Biolabs, Inc , Beverly, MA), Novagen (Novagen, Inc , Madison, WI) EXAMPLE 1 Direct Detection of Integration by PCR In order to test whether Rep expression products facilitate integration of an AAV vector plasmid when transduced into a mammalian target cell, the following experiment was conducted A stable human cell line, 293, (readily available through, e g , the American Type Culture Collection under accession number ATCC CRL1573) was grown to approximately 75% confluency in medium consisting of DMEM with 10% FBS and Pen/Strep The 293 cells were then transfected with an AAV-LacZ vector plasmid (pABl l), using the calcium phosphate precipitation method pAB 1 1 was deposited with the American Type Culture Collection, pABl l was assigned ATCC No. . The AAV-LacZ vector, in a cassette cloned in the P.stl site of pGEM-4 (Promega), included the AAV ITRs flanking a CMV promoter driving the LacZ gene The 293 cells were also transfected with AAV helper plasmids containing either the rep gene (pGN1980) or the rep and cap genes (pGN1764) or with the AAV-LacZ plasmid (pAB 1 1 ) as a control The rep containing plasmid included the AAV rep coding region (+145 to +2942, Sπvastava et al J Virol (1983) 45 555-564) cloned in the Sma site of pUC19 (New England Biolabs) The rep and cap containing plasmid included the same insert as pAAV-Ad (Samulski et al J Virol (1989) 63 3822-3828) cloned in the Notl site of pBSII KS- (Stratagene) Neither plasmid included the AAV ITRs Shortly after the transfection, the 293 cells were infected with a freeze-thaw extract of adenovirus type 2 Three days later, total DNA was prepared from the cells If site specific integration has occurred, it should be possible to detect it by PCR, using a pair of primers, one complementary to the AAV ITR and the other complementary to a region of chromosome 19 adjacent to the preferred AAV integration site The PCR amplified DNA would be expected to be heterogeneous (Figure 2) as template DNA is obtained from a pool of cells in which many integration reactions have occurred and as AAV integration sites in this region are spread over several hundred base pairs However, after recutting the PCR amplified product with Sacl, a 300 bp fragment should be generated This fragment should hybridize to a Sac/Bam probe derived from the AAVSl region In other words, the presence of a 300 bp band on a Southern would be indicative of integration Thus, a PCR reaction was conducted as described above For the PCR reaction, 200μM dNTPs were combined with 2μM of each primer, in lx PCR buffer (Bochr Kit), l OOng DNA and 1 unit of Taq polymerase The amplification cycle proceeded for 15 seconds at 98°C, 5 minutes at 94°C, 35 cycles for 1 minute at 94°C, 1 minute at 55°C, and 4 minutes at 72°C The PCR product was recut with Sαcl by mixing lOμL of amplified DNA with 2 units of Sαcl in 2 μL buffer A (lOx) and 8 μL H 2 O The mixture was incubated at 37°C for 1 hour to generate a 300 bp fragment which was amplified and resolved by electrophoresis and transferred to nitrocellulose DNA from the cells cotransfected with both the AAV-LacZ and one or both of the helper plasmids hybridized to a 300 bp Sac/Ba probe derived from the AAVS 1 region, indicating that site-specific integration had occurred EXAMPLE 2 Increased Frequency of Integration in the Presence of Rep Protein To test the hypothesis that Rep proteins increase the frequency of integration of an AAV plasmid vector into the chromosomal DNA of target mammalian cells, the following experiment was done 293 cells were transfected either with an AAV-neo plasmid vector, pWP8-AAV-TK- neo, alone or cotransfected with pWP8 and either the rep containing plasmid (pGN1980) or rep and cap containing plasmid (pGN1764), both described above To determine stable integration of the vector, the number of neo resistant colonies were counted for each vector construct as follows Cells were placed under G418 selection (400 μg/mL) 48 hours after transfection Media was replaced every 3 days Plates were stained with a staining solution of 2% methylene blue 2% and 50% EtOH After 2 minutes, the monolayers were washed and the stained clones counted The number of G418 recombinant colonies increased 3x in the presence of pGN1980 and 3 Ox in the presence of pGN1764 The above experiments indicates that efficient integration occurs in the target cell in the presence of Rep expression products In both cases, the effect was more pronounced in the presence of pGN1764, suggesting that cap might stimulate this reaction Alternatively, pGN1764 might simply express Rep better than pGN1980 Furthermore, the first experiment shows that at least some of the integration events are target specific as one of the two primers is homologous to AAVSl EXAMPLE 3 Identification of DNA Sequences Required For Integration The previous examples have indicated that rep expression products facilitate integration of an AAV vector plasmid into a mammalian target cell In order to identify the genetic elements in AAV vectors that contribute to integration, derivatives of pAAV carrying various mutations affecting the AAV coding region were tested Transfection experiments were conducted with the pAAV mutants Three days after transfection, genomic DNA was prepared and submitted to a PCR dot blot procedure Cell Lines Both the human embryonal kidney cell line, 293, and HeLa cells were used in the experiments of this example The 293 cells are readily available through, e g , the ATCC (ATCC accession number CRL1573) The HeLa cells are also readily available through the ATCC (e g , number CCL2) Plasmid Provirus And Mutants Referring to Figure 3, pAAV is a plasmid carrying a replication-competent AAV provirus pAAV is identical to psub201 , the structure of psub201 is provided in U S Patent No 5,436,146 to Shenk et al which is hereby incorporated by reference Four mutants of pAAV were constructed pAAVMl, pAAVM2, pAAVM3, and pAAVM4 (Figure 3) pAAVMl contained a deletion in the cap region between Apal sites at position 2943 and 4040 The other three derivatives (/ e , pAAVM2, pAAVM3, and pAAVM4) contained insertions in the rep region To construct pAAVM2, an 8 bp Ascl linker (denoted by the *) was inserted in the Nm site located on pAAV, this insertion results in a -1 frameshift affecting the p5-deπved Rep 78 and Rep 68 proteins (the long forms of Rep) pAAVM3 was generated by treating BamHl-cut pAAV with Klenow enzyme (Boehringer) to fill in the ends, the blunt ends were then ligated together using standard ligation techniques, described above The * represents the filled-in Ba Hl site Because the BamHl site is situated downstream of both promoters, this mutation in pAAVM3 results in +1 frameshift affecting all four forms of Rep (/ e , both the two short forms and the two long forms) pAAVM4 is a double mutant carrying both frameshift mutations (/ e , the frameshift mutations of pAAVM2 and pAAVM3) The two * symbols represent the position of the Ascl linker and the filled-in BamHl site Transfection of 293 cells The transfection procedure was performed according to the procedure that follows The conditions were the same for both 293 cells and HeLa cells First, for each experimental condition, 2 mL of cells were plated at a concentration of 4 x lOVmL in each well of a six-well plate The culture media consisted of DMEM (Gibco #12-614F), 10% FBS (Hyclone # A-l l l-L), 50 units/mL Penιcιllm/50 units/mL streptomycin (Gibco #15070-014) and 2 mM L-glutamine (BioWhittaker #17-605E) The cells were incubated overnight at 37°C in an atmosphere containing 5% CO 2 Second, a calcium phosphate-DNA precipitate was prepared immediately before transfection For each condition, 224 μL of Milli-Q (Class III) water were added Next, 26 μL of 2 5 M CaCl 2 were added, followed by 2 μg of each DNA While gently mixing the DNA mixture, 250 μL of 2x HBS (274 mM NaCl, 10 mM KC1, 1 4 mM Na 2 HPO 4 , 12 mM dextrose, 42 M Hepes, pH = 7 05) were added This precipitate was added to each well (/ e , each experimental condition) dropwise Third, six hours later, the media and precipitate were aspirated from each well Two mL of growth media were gently added, taking care not to disturb the cell monolayer Next, the media was aspirated from the well, and 2 mL of fresh growth media were added The plate was then returned to the incubator Extraction of Genomic DNA After three days in culture, total genomic DNA was extracted from the pool of transfected cells Genomic DNA was isolated for PCR and digestion by the same procedure, described below First, cells were trypsinized, then diluted in medium containing FBS to inactivate the trypsin and the cells were pelleted, the cell pellets were then resuspended in TE Next, 0 5 mL of digestion DNA buffer (50 mM Tπs, pH = 8, 20 mM EDTA, 0 1 M NaCl, 1% SDS) and Proteinase K at a final concentration of 200 μg/mL (/ e , a 1 100 dilution of 20 mg/mL stock) were added The digestion buffer was added at approximately 0 5 mL/1-5 x 10 6 cells or 0 5 mL/well of a six-well plate, for larger cell volumes, e g , 10-20 x 10 6 cells, 2 5 mL per T75 flask or 10 cm dish can be added in a 15 mL tube The cells were then incubated at 37°C for approximately 1 hour Phenol/chloroform extraction was then performed (for larger preparations, the phases can be separated at 3000 rpm in 15 mL tubes in a Beckman GS-6R table top centrifuge) The upper aqueous phases were ethanol precipitated with 0 1 volumes of 3 M sodium acetate and 3 volumes of ethanol at -80°C for approximately 15 minutes It was necessary to vigorously shake the preparations to ensure that the DNA was well mixed The DNA was then formed into a pellet by microfuging at full speed for 15 minutes at 4°C (for larger preparations, the DNA can be pelleted at 7000 rpm at 4°C) Thereafter, the DNA was washed with 70% ethanol Finally, the DNA was resuspended in 100 μL of TE and RNase A at 10 μg/mL (for 1 - 5 x 10 6 cells in a six-well plate). For larger preparations, e.g., 10-20 x 10 6 cells (T75 flask or 10 cm dish), 500 μL of TE and RNaseA can be used. In addition, for larger preparations, DNA may require an overnight incubation at 37°C, with rotation, to enable the DNA to get into solution. Description of Primers and Probes for PCR Dot Blot Procedure Figure 4 is a schematic map of the AAVSl region of chromosome 19 depicting an AAV derivative {i.e., a provirus) integrated into the AAVSl region (the region is labeled as chr.19-AAVS1 in Figure 4). As previously noted, the integration locus for AAV is human chromosome 19q l3.3-qter; this is termed the "AAVS l region". Samulski et al. (1991 ) EMBO J. 10:3941-3950; Kotin et al. (1992) EMBO J. 11:5071-5078. Referring to Figure 4, the inverted viral terminal repeats are symbolized by open rectangles containing solid triangles; the points of the triangles indicate the direction or orientation of the ITR such that the triangles point away from the recombinant vector sequence. An expanded view of one of the ITRs is shown by the open rectangle containing the letters a, b, c, and d; each letter represents a discrete nucleic acid sequence. As discussed in further detail below, primer 100 is derived from the a',c' region of the AAV ITR and primer 81 is derived from the d region of the AAV ITR. The letters a', b', and c' represent the inverse sequence found in a, b, and c, respectively. In Figure 4, the location of primers complementary to sequences of the AAVS 1 region or the integrated AAV derivative are indicated by arrows. The tip of the arrow represents the 3' end of the each primer. Primers 79, 80, and 97 are derived from AAVSl The sequence of these primers are as follows: Primer 79: 5'-ACTTTGAGCTCTACTGGCTTC-3' (SEQ ID NO 1 ); Primer 80: 5'-GGAGGATCCGCTCAGAGG-3' (SEQ ID NO:2); and Primer 97: 5'-CGGGGAGGATCCGCTCAGAGGTACA-3' (SEQ ID NO 3). As noted above, primers 81 and 100 are derived from {i.e., are complementary to) the AAV ITR. Kotin et al. (1992) EMBO J. 11:5071-5078. The sequence of Primer 81 is 5'-AGGAACCCCT AGTGATGGAGT-3' (SEQ ID NO:4), and the sequence of Primer 100 is 5'-CGGCCTCAGTGAGCGAGCGCGC-3' (SEQ ID NO:5) Finally, Primer 98, derived from the p5 promotor, has the following sequence: 5'-CGCGTTCAAACCTCCCGCTTCAA AATG-3' (SEQ ID NO:6). The location of a probe (double stranded DNA), a SacllBamHl restriction fragment, complementary to the AAVSl region is indicated by line labeled "probe" in Figure 4 PCR Dot Blot The PCR Reaction A PCR assay was used to detect site specific integration in unselected pools of transfected cells If site specific integration has occurred, it should be possible to detect it by PCR, using a pair of primers, one complementary to the AAV ITR and the other complementary to the AAVS l region of chromosome 19 (/ e , 19ql 3 3-qter) The PCR amplified DNA would be expected to be heterogeneous (See Figure 2), as template DNA is obtained from a pool of cells in which many integration reactions have occurred, moreover, wild type AAV is believed to integrate in a region of 19ql3 3-qter spanning several hundred base pairs However, all specific PCR amplification products, irrespective of their size, should hybridize to a Sacl-BamHl fragment derived from the AAVS l region Thus, specific amplification products can be detected by DNA hybridization on dot blots using a SacllBamHl probe derived from AAVSl (Figure 4) The PCR reaction was performed according to the procedure described hereafter An aliquot of DNA corresponding to 10 4 genomes (cells) was used in the PCR reaction For the PCR reaction, lOOμM dNTPs were combined with lμM each of primers 97 and 100 in IX NEB buffer for vent polymerase supplemented with 2 mM MgSO 4 , and 2 units of Deep Vent R * (exo) DNA polymerase (NEB) The cycle conditions were 10 seconds at 99° C and 4 minutes at 72° C for a total of 35 cycles For the Dot Blot procedure, 10% of the PCR reaction product was transferred to a nylon Zeta-Probe blotting membrane (Biorad) using a dot blot apparatus That sample was then hybridized, according to the manufacturer's protocol, to the probe (/ e , the SacllBamHl restriction fragment, derived from the AAVSl region of chromosome 19) The probe comprised a random primed 32 P labeled PCR fragment The probe fragment was first generated by PCR, then labeled The PCR comprised 0 1 μg of human genomic DNA and the primers were primer 79 and primer 80 The PCR conditions were as follows lx laq buffer, 0 2 mM dNTP in a total volume of 90 μL The PCR amplification was performed using Taq polymerase as follows 1 cycle at 99°C for 5 minutes, pause at 75°C, 97°C for 5 seconds, 3 cycles at 97°C for 10 seconds, 55°C for 10 seconds, and 72°C for 10 seconds, 32 cycles at 95°C for 10 seconds, 55°C for 10 seconds, and 72°C for 10 seconds The labeling was conducted by random priming, as described above Validation of the PCR Assay The PCR assay was validated using wild type AAV to infect the 293 cells and the HeLa cells. Initially, it should be noted that all of the results depicted in Figures 5A-B, 6, and 9 were obtained using the same pair of primers: primer 97 and primer 100 However, a signal (i.e., probe hybridization) was detected whether the virus-specific primer was homologous to the a,c region of the ITR (primer 100), the d region of the ITR (primer 81), or to the unique internal region of the virus (primer 98). The primers specific for the AAVSl region of chromosome 19 (the region is labeled as chr.19-AAVS1 in Figure 4) were also used successfully; these were primers 79, 80 and 97. Figure 5A depicts the results of 293 cells infected with wild type AAV2 at three different multiplicities of infection (MOIs); Figure 5B depicts the results obtained from analysis of HeLa cells infected with wild type AAV2 at three different MOIs - l θ\ 10\ and 10 5 . As used herein, the term "MOI" is the number of recombinant AAV virions used to infect each cell as detected by determining the number of single stranded genomes by quantitative DNA hybridization. Unless otherwise indicated, the results presented in Figures 5A and B were obtained using the same procedures. Referring to Figures 5A and B, a signal indicative of site specific integration was detected in both 293 cells and HeLa cells at all three MOIs tested; however, no signal was detected in the mock-infected cells. The Results of the Transfection Experiments The results of the transfection experiments are depicted in Figure 6. As one would expect, the cells transfected with pAAV indicate that integration has occurred. The pAAVMl proviral construct, containing an approximately 1 kb deletion in the cap gene, was able to integrate site specifically into AAVSl . In contrast, all frameshift mutations affecting the rep gene displayed significantly decreased levels of site specific integration. These results demonstrated that the rep gene plays a critical role in targeting a mutant provirus to the AAVSl region of chromosome 19 {i.e., 19q 13.3-qter). EXAMPLE 4 Rep Expression in trans Mediates Site-Specific Integration Of Large Pieces of DNA The experiments in Example 3 identified those DNA sequences required for integration. The experiments in this example are directed at indicating that rep expression in trans mediates site specific integration of large pieces of DNA Preparation of the Constructs The Targeting Vectors Figure 7A depicts diagrams of the targeting vectors used in this example. In two of the targeting vectors (p2TRlacZ and p2TRF8), the rep and cap genes of pAVV (Figure 3) were removed and replaced by (i) an expression cassette comprising the CMV promoter fused to the lacZ gene (p2TRlacZ), and (ii) an expression cassette comprising the CMV promoter fused to the human factor VIII cDNA (p2TRF8), respectively. For the third targeting vector (p2TRcos), an ITR-kan r -ITR cassette was inserted into a yeast cosmid. Details regarding the construction of these vectors are presented below. p2TRlacZ is a derivative of psub201 (see U.S. Patent No. 5,436, 146 to Shenk et al.) in which the Xba\ fragment carrying the rep and cap genes present in psub201 were replaced with an expression cassette composed of the CMV promotor fused to the lacZ gene. p2TRlacZ was constructed according to the following procedure. First, an oligonucleotide encoding the restriction enzyme sites Notl-Mlul-SnaBl-Agel-BstBl-BssHll-Ncol-Hpa -BspEl- Pmfl-RsrU-Not (5'-GCGGCCGCACGCGTACGTACCGGTTCGAAGCGCGCACGG CCGACCATGGTTAACTCCGGACACGTGCGGACCGCGGCCGC-3') (SEQ ID NO 7) was synthesized and cloned into pUC19 cut with Kasl-Earl(pa ti_\) and blunted, producing a 2757 bp vector fragment. Second, three fragments were cloned into various sites of that 2757 bp vector fragment: (i) a 653 bp Spel-Sacll fragment encoding the CMV IE promoter was cloned into the SnaBl site; (ii) a 269 bp PCR-produced -9stBI--9.s'tBI fragment encoding the first intron of the hGΗ gene (the primers used were as follows: 5'-AAAATTCGAACAGGT AAGCGCCCCTTTG-3' (SEQ ID NO:8) and 5 -AAAATTCGAACCTGGGGAGAAACC AGAG-3' (SEQ ID NO:9)) was cloned into the βstBI site; and (iii) a 135 bp Hpal-Ba l (blunted) fragment containing the SV40 early polyadenylation site from pCMV-β was cloned into the Hpά site. Third, the resulting plasmid was cut with Notl and the CMVlacZ expression cassette was cloned into the psub201 vector fragment (containing the bacterial ori and amp r genes) which had been cut with Xbal, blunted, and linked with Notl linkers this procedure resulted in the plasmid psub201CMV Fourth, psub201CMV was then cut with -9s.vFffl(partιal), a 3246 bp adhlacZ gene contained on a Smal-Dral fragment derived from the plasmid pCMV-β (Clonetech) and linked with Ascl linkers (5'-GAAGGCGCGCCTTC-3 ') (SEQ ID NO 10), was hgated to the -9.«HII-cut psub201CMV to create p2TRlacZ It should be noted that because all of the linkers were obtained as single stranded DNA (in lysopholized form), the sequence identification numbers (SEQ ID NOs) list the linkers as being single stranded DNA p2TRF8 is identical to p2TRlacZ with the exception that the lacZ gene has been replaced with human factor VIII cDNA p2TRF8 was constructed by replacing the adhlacZ gene in p2TRlacZ with human factor VIII cDNA (8968 bp) through the use of Ascl linkers (using the same procedure as described above The total size of this plasmid is approximately 14 kb, and the distance between the two ITRs is approximately 9 5 kb p2TRF8 was deposited with the American Type Culture Collection, p2TRF8 was assigned ATCC No p2TRcos is a derivative of cPM9214, a cosmid obtained from the ATCC (# 70892) that consists of 28,010 base pairs of yeast DNA from the left arm of chromosome III cloned into the BamHl site of pHC79 Cosmid cPM9214 contains a unique £c/136 II site This unique Ec/136 II site was cut, and Notl linkers (5'-TTGCGGCCGCAA-3') (SEQ ID NO 1 1) were gated to the ends, thereby creating a new cosmid (pRR23) containing a unique Notl site Thereafter, pRR23 was cut with Notl, and a Notl restriction fragment carrying an ITR- kan r -ITR cassette (described below) was inserted in the Notl site, thereby creating cosmid p2TRcos The ITR-kan r -ITR cassette inserted into the Notl site was constructed as follows The ITRs from psub201 were modified to add Sse I and Notl linkers in order to achieve the following structures 5'ITR Ssel- wII-ITR-.Ybαl-Notl, 3' ITR NotI--YZ>αI-ITR-.Pv..II-SseI Next, a 1772 bp fragment containing the kan r gene was obtained from plasmid pBK/CMV (Stratagene) by digestion with BspHl, this fragment was then treated with Klenow enzyme in the presence of dNTPs and Ssel linkers were hgated to each end After digestion with Ssel, the fragment was gated to the AAV ITRs described above to obtain the fragment 5 '-Notl - Xbal-πR-P\>ull-Ssel-km'-Ssel-Pvull-lTR-Xbal-Noll-3 , {ι e , the ITR-kan r -ITR cassette) This cassette was then inserted into the Notl site of pRR23, as described above Figure 8 diagramatically depicts the methods used in the construction of p2TRcos It should be noted that the orientation of the ITRs relative to the kan gene is opposite the orientation of the ITRs to the rep and cap genes in an AAV provirus. The Helper Plasmids Figure 7B depicts diagrams of the helper vectors used in this example In Figure 7B, the following abbreviations and symbols are used the open rectangle labeled "rep" in its interior (coding region, i.e., open reading frame, for the rep gene); the open rectangle labeled "cap" in its interior (coding region, i.e., open reading frame, for the cap gene), the solid triangles with a vertical line attached (location of the endogenous promoters for encoding p5, pi 9, and p40 proteins) See, e.g., Muzyczka, N. (1992) Current Topics in Microhiol. and Immunol. 158:97-129, Berns, K (1990) Microhiol. Rev. 54(3).316-29 Two AAV helper plasmids were constructed, (i) pGN1764, which includes the AAV rep and cap coding regions (described in Example 1), and (ii) pRR5 , which includes the AAV rep coding region and only part of the cap coding region pGN1764 contains the entire AAV coding region This plasmid included the same insert as that found on pAAV-Ad (Samulski et al. J. Virol. (1989) 63 3822-3828) isolated as a Notl restriction fragment cloned in the Notl site of pBSII KS- (Stratagene) All three endogenous promoters (p5, pi 9 and p40, indicated by arrows in Figure 7B) are present in this construct pRR5 was constructed as follows First, psub201 was digested with Apal, the 1 103 base pair fragment in the cap region was removed, and then the two ends were religated The resulting construct was then digested with Xbάl, to create a 3 2 kb AAV genomic fragment That 3.2 kb fragment was subsequently inserted into the Spel site of p680E3 (Ketner et al., A (1994) PΝAS 9_i(13):6186-90) to produce pRR5 Again, all three endogenous promoters (p5, pl9 and p40, indicated by arrows in Figure 7B) are present in this construct; the Apal restriction site is also depicted in Figure 7B Transfection of 293 Cells Human embryonal kidney cell line 293 was used in this experiment This experiment tested whether cotransfection of a targeting vector with a Rep-expressing plasmid was sufficient to cause site specific integration of the targeting vector, containing large nucleotide sequences, whose only AAV-derived sequences are the two ITRs In this experiment, three ITR-containing vectors (described above) were used (i) p2TRlacZ, an expression cassette comprising the CMN promoter fused to the lacZ gene, (ii) p2TRF8, an expression cassette comprising the CMV promoter fused to the human factor VIII cDΝA; and (iii) p2TRcos, a cosmid containing a ITR-kan r -ITR cassette in which the orientation of the ITRs relative to the kan gene is opposite the orientation of the ITRs to the rep and cap genes in an AAV provirus. These plasmids were transfected either alone or along with either one of two Rep-expressing plasmids (pGΝ1764 and pRR5). The results of this experiment are depicted in Figure 9 and described below Cell Cultures and Extraction of Genomic DNA The protocols for culturing the 293 cells and for extracting the genomic DNA were identical to the protocols set forth in Example 3. After three days in culture, total genomic DNA was extracted from the transfected cells. An aliquot of DNA corresponding to 10 4 genomes was used in a PCR Dot Blot procedure. The PCR reaction and Dot Blot procedure was also performed identically to the description provided in Example 3. Site Specific Targeting As depicted in Figure 9, site specific integration did not occur when the three ITR- containing plasmids (i.e., p2TRlacZ, p2TRF8, and p2TRcos) were transfected into 293 cells alone; the lack of site specific integration is indicated by the absence of a strong signal However, cotransfection with both Rep-expressing plasmids {i.e., pGN1764 and pRR5) resulted in the integration of all three ITR-containing plasmids The results indicate that rep expression in trans mediates site-specific integration Moreover, the integration of p2TRF8 and pTRcos indicates that it is possible to target large pieces of DNA that play an important role in disease states and the like. Indeed, it might be possible to integrate DNA sequences of approximately 100 kb or more EXAMPLE 5 Confirmation of Chromosomal Location The experiments described in this example are directed at confirming the chromosomal location of the integrated target vectors. More specifically, the experiments in this example indicate that site specific integration occurs into the AAVSl region of chromosome 1 Preparation of Nucleic Acid Constructs The following constructs were used in this example the target vector p2TRlacZ and the helper plasmids pRR5 and pRSVRepCap The p2TRlacZ and pRR5 were constructed as described in Example 4 pRSVRepCap was constructed as described below pRSVRepCap was constructed according to the following procedure First, a Bgfl linker was inserted into the AAV genome (pGN1764) at the HΛαl site eleven nucleotides 5' to the ATG codon of Rep 78, this insertion resulted in the creation of pGN1782 pGN1782 was then digested with Bg/ll and Xbal to yield a 4174 bp fragment containing the rep and cap coding sequences, but lacking the p5 promoter This fragment was then treated with Klenow enzyme in the presence of dNTPs and hgated with Notl linkers Thereafter, the fragment was digested, with Notl, gel purified, and hgated into the Notl site of pORSVICAT (Stratagene) The resulting plasmid, pROS-001, contained the RSV promoter immediately 5' of the ATG of Rep 78 pRSVRepCap was constructed from pROS-001 First, pROS-001 , which contains a unique SnaBl site, was digested with SwαBI Then, an Xba -Hm W fragment was derived from the simian virus 40 (SV40) origin of replication by digesting with Xbal and HwDIII This -YbαI-H//?DIII fragment was then treated with Klenow enzyme in the presence of dΝTPs and inserted into the unique SnaBl site of pROS-001 , resulting in pRSVRepCap β-galactosidase Protocol The production of β-galactosidase by cells allowed detection of those cells successfully transfected in order to pick clones of cells that could then be expanded The following reagents, supplies, and methods were used in the β-galactosidase procedure described below Tissue culture reagents and supplies (l) DMEM (Bio-Whittaker cat # 12-614F), containing 10% fetal calf serum (ΗyClone cat #A1 1 1 1-L, heat-inactivated at 56°C for 1 hour), 50 units/mL of penicillin G, 50 units/mL of streptomycin (GibcoBRL cat #15070-014) and 2 mM L-glutamme (Bio-Whittaker cat #17-650E), (u) Dulbecco's phosphate-buffered saline without calcium and magnesium (Hyclone cat #B-4004-L), (in) 0 25% trypsin solution with EDTA (Hyclone Cat #B-3004-D), (IV) 75 cm 2 tissue culture flasks (Corning cat #430641 ), and (v) 12-well tissue culture plates (well diameter 2 2 cm, Corning cat #25815) Assay reagents (I) fixative solution The fixative solution consisted of Dulbecco ' s phosphate buffered saline without calcium and magnesium (HyClone catalog-B-4004-L) containing 2% formaldehyde and 0 2% glutaraldehyde The solution was prepared by combining Dulbecco's PBS, 25% aqueous glutaraldehyde, and 37% formaldehyde The fixative solution was prepared fresh on the day it was used (π) staining buffer The staining buffer consisted of Dulbecco's phosphate buffered saline without calcium and magnesium (HyClone cat -B-4004-L) containing 5mM K 3 Fe(CN) 6 , 5mM K 4 Fe(CN) 6 , and 2mM MgCI 2 This solution was prepared from the following stock reagents Dulbecco's PBS, 200 mM K 3 Fe(CN) 6 , 200 mM K 4 Fe(CN) 6 , and 1M MgCl 2 (store the 200 mM K,Fe(CN) 6 and K 4 Fe(CN) 6 solutions in the dark at 4°C) The staining buffer solution was prepared fresh on the day it was to be used (in) X-gal stock solution The X-gal solution (5-bromo-4- chloroindolyl-β-galactaside) consisted of 40 mg/mL X-gal in dimethylformamide The solution was stored at -20°C in the dark (iv) substrate solution Staining buffer with a final concentration of 1 mg/mL X-gal Prepare no more than 30 minutes before use Fixation and staining of cells The reagents were prepared in the following manner (i) fixative solution for 100 mL, 0 8 of 25% aqueous glutaraldehyde and 5 4 mL of 37% formaldehyde were added to 93 8 mL of dulbecco's PBS The fixative solution and an equal volume of Dulbecco's PBS (for rinse) were placed on ice, (π) staining buffer for 100 L, add 2 5 mL of 200 mM K 3 Fe(CN) 6 , 2 5 mL of 200mM K 4 Fe(CN) 6 , and 0 2 mL of 1 M MgCl 2 were added to 94 8 mL of Dulbecco's PBS, and (in) substrate solution this solution should be prepared no more than 30 minutes before use For 100 mL, 97 5 mL of staining buffer were warmed to 37°C, 2 5 mL of X-gal stock solution (40 mg/mL X-gal in dimethylformamide) were added, and the resulting solution was mixed well This solution was kept at 37°C until used Each well of the 12-well tissue culture plate to be stained required 1 mL of fixative solution, 1 mL of Dulbecco's PBS, and 1 mL of substrate solution First, the medium was aspirated from the wells, 1 mL of cold fixative solution was added, and then a 5 minute incubation period was commenced Second, the fixative solution was aspirated, the wells were washed once with 1 mL of Dulbecco's PBS, and then replaced with 1 mL of warm substrate solution Finally, the plate was incubated for 24 hours at 37°C (do not use a CO 2 incubator) in the dark (wrapped in aluminum foil) Although the cell cultures began to stain immediately, they required 24 hours to fully develop before counting Transfection of 293 Cells Human embryonal kidney cell line 293 were used in this experiment Pools of cells were cotransfected with either p2TRlacZ and pRR5 or with p2TRlacZ and pRSVRepCap Referring to the results of the Southern Blot depicted in Figure 10, lane 4 represents cells cotransfected with p2TRlacZ and pRR5, lanes 1-3 and 5-7 represent cells cotransfected with p2TRlacZ and pRSVRepCap, and lane 8 is the control (293 cells) During the post-transfection period between day 2 and day 28, single cell clones were isolated from pooled populations by FAC sort or limiting dilution These single-cell clones were then expanded, and the clones were screened for β-galactosidase production Production of β-galactosidase, resulting from the expression of the lacZ gene, was determined using the materials and methods described above Seven clones positive for β-galactosidase were chosen at random and expanded. Preparation of Genomic DNA Extracts Genomic DNA (10 μg from each clone) from the seven β-galactosidase positive clones was restricted with HinOlll, a restriction enzyme that has no restriction site in either the AAVS l region or in p2TRlacZ The resulting digests were then resolved by electrophoresis on a 0.8% agarose gel (Gibco-BRL), and the product was transferred to a nitrocellulose membrane (Schleicher and Schuell) Southern Blot Analysis The genomic DNA extracts were then analyzed by Southern blot using an AAVSl probe (a 3 5 kb EcoRl-Kpnl fragment derived from the AAVSl region of chromosome 19) Figure 10, a Southern blot, depicts the results of the hybridization analyses, each of lanes 1-7 contained the genomic DNA extract from one of the β-galacatasidase-positive clones An understanding of the significance of restricting the DNA with HinOlll is helpful when reviewing the results in Figure 10 While the exact location of the HinOlll sites are unknown, it is known that a H/..DIII restriction site lies on either side of the AAVSl region, however, there is no H/..DIII site within that region Thus, hybridization of non-transfected 293 cells with the probe yielded, as expected, a single band (Figure 10, lane 8, 6 6 kb) Conversely, the site specific integration of DNA sequences by transfection introduces additional HinOlll sites in the AAVSl region Therefore, more than one restriction fragment from the AAVS 1 region should be formed upon digestion with H./.DIII, and more than one band should be visible when probed with the AAVSl fragment Reference to Figure 10 reveals that a genomic event (indicated by the > symbol) appeared in lanes 1 and 3-7 That is, site specific integration occurred in the clones in each of those lanes The rearrangements of the AAVSl region are different in each clone, as indicated by the different positions of the additional hybridization bands in each of those lanes. In one case (lane 4), three AAVS 1 hybridizing bands are visible in addition to the single band present in the parent 293 cells. The genomic DNA from that clone was subsequently used in the fluorescent in situ hybridization, described below. Characterization of the Position and Structure of the Integrated p2TRlacZ The position and structure of the integrated p2TRlacZ in one of the seven β- galactosidase expressing clones was characterized in two independent ways. First, using fluorescent in situ hybridization, the integration event was mapped to one homolog of chromosome 19 at position 19ql3.4. Second, the integrated sequences were cloned, taking advantage of the fact that the entire p2TRlacZ {i.e., the colEl origin and the amp selectable marker) appears to be integrated. Both of these procedures and the results obtained are discussed in detail below. Fluorescent in situ Hybridization Fluorescent in situ hybridization was performed in order to definitively show that p2TRlacZ was linked to the AAVSl region. The β-galactosidase-positive clone (cotransfected with pRR5) that yielded three hybridizing bands in the Southern blot was used in this analysis (Figure 10, lane 4). Metaphase chromosomes were prepared using standard cytogenetic techniques from cell line 293 lac z (CRL1573). Purified DNA from plasmid clone p2TRlacZ was labeled with digoxigenin-dUTP by nick translation. Labeled probe (p2TRlacZ) was combined with sheared human genomic DNA and cohybridized to metaphase chromosomes with a probe from the E2A locus (Genome Systems, Inc.). Hybridization was carried out in a solution containing 50% formamide, 10% dextran sulfate, and 2X SSC Specific hybridization signals were detected and amplified by the sequential application of fluoresceinated sheep antidigoxigenin antibodies (commercial source; used by Genome Systems, Inc.) and fluoresceinated rabbit antisheep antibodies (commercial source; used by Genome Systems, Inc.); this was followed by counterstaining with propidium iodide. The method is diagrammatically depicted in Figure 1 1. Referring to Figure 1 1, the p2TRlacZ probe was used to mark the integration site of p2TRlacZ in the AAVS 1 region of chromosome 19; the E2A probe was used to mark the p arm of all of the copies of chromosome 19 present in the cell {i.e., the E2A probe specifically identifies the p arm on chromosome 19) This experiment resulted in the labelling, by the E2A probe, of four copies per cell of chromosome 19 at 19pl3 and the labelling, by the 2TRlacZ probe, of 1 copy of chromosome 19 at 19q l3 4. These results indicate that p2TRlacZ is integrated into a single homologue of chromosome 19 at a position near the q terminus All copies of chromosome arm 19p were marked with the hybridization signals from the E2A probe, which demonstrated that the p2TRlacZ integration site is on the q arm of chromosome 19 Cloning of the Integrated Sequence The integration of p2TRlacZ was also physically demonstrated by sequence analysis The β-galactosidase-positive clone (cotransfected with pRR5) that yielded three hybridizing bands in the Southern blot was also used in this analysis (Figure 10, lane 4) ("clone 4") As described above, approximately 10 μg of genomic DNA from clone 4 was cut to completion with H /.DIII (which does not cut p2TRlacZ), and the digestion products were resolved on a 1% agarose gel (Gibco-BRL) Thereafter, the digestion products that were larger than 8 kb, the approximate size of p2TRlacZ, were purified using the Geneclean Kit (BIO 101 , Inc ) The purified material was ligated in dilute conditions (2 μg/mL) to allow circularization of the rescued plasmid and surrounding genomic DNA and transformed by electroporation in 293 cells (E. co/i strain DΗ10B) [See, e.g., Current Protocols Mol Biol 1 1 8 1 -1 8 8 (1994) John Wiley & Sons, Inc.] As discussed further below, the resulting plasmid was characterized and the structure of the integrated plasmid sequences were determined along with the nature of the integration site. Reference to Figures 12A-C will assist in understanding the discussion that follows Figure 12A diagrammatically depicts several of the characteristics of the AAVS l region of chromosome 19. Referring to Figure 12A, the AAVSl region is represented by the large open rectangle (labeled "AAVS l "). Two restriction enzyme sites within the AAVS l region are indicated by the vertical lines protruding from that region {i.e., E oRl and Kpn\ , located 1 bp and 3530 bp downstream of the 5' end of the AAVSl region, respectively) The binding site for the Rep expression product is represented by the shaded square (labeled "RBS") within the AAVSl region; this binding site occupies base pairs 405-416 of the AAVSl region. Figure 12B diagrammatically depicts the plasmid p2TRlacZ. As previously noted, p2TRlacZ is a derivative of psub201 (see U.S. Patent No. 5,436, 146 to Shenk et al. ) in which the Xbal fragment carrying the rep and cap genes present in psub201 were replaced with an expression cassette composed of the CMV promotor fused to the lacZ gene. Referring to Figure 12B, the psub201 vector fragment is indicated by the solid region, and the approximate positions of the bacterial ori and amp r genes within that fragment are labelled "ori" and "amp," respectively. Each of the two ITRs is depicted as an open box containing a shaded arrowhead. Finally, the lacZ gene is indicated by the slanted lines and the fused CMV promoter is indicated by the open region connecting the /acZ gene to one of the ITRs Cells from human cell line 293 were cotransfected with p2TRlacZ (Figure 12B) and with helper plasmid pRR5 (not shown), resulting in the chromosome 19 integration product shown in the top portion of Figure 12C. Figure 12C uses the same designations that were used in Figures 12A and B Briefly, the AAVS l region is represented by the large open rectangle (labeled "AAVS l ") The psub201 vector fragments of p2TRlacZ are indicated by the shaded rectangles, and the approximate positions of the bacterial ori and amp r genes within those fragments are labelled "ori" and "amp," respectively. Each ITR is depicted by an open box containing a shaded arrowhead. Finally, the lacZ gene is indicated by the slanted lines, and the fused CMV promoter is indicated by the open rectangles (labeled "CMV") connecting the lacZ gene to an ITR (the junction between each CMV promoter and each ITR is indicated by the Spel restriction site). The top portion of Figure 12C also contains information relating to other restriction enzyme sites (i.e., HinOlll and E oRV), indicated by the vertical lines protruding from the chromosome 19 integration product. As set forth above, digestion of genomic DNA isolated from the transformed (clone 4) cells with HinOlll was performed, indicating that approximately 1.7 tandem copies of p2TRlacZ were integrated (Figure 12C). The right and left junctions between plasmid and genomic DNA were sequenced The sequence of the right junction was compared to the nucleic acid sequences of the lacZ gene and a portion of the AAVS 1 region. The nucleic acid sequences are set forth in the bottom right-hand portion of Figure 12C and are as follows: (i) right junction: 5' - GCCAGTCTGGGCGGG - 3' (SEQ ID NO:12); (ii) lacZ gene: 5' - GCCAGTCAGGCTTT CTTT - 3' (SEQ ID NO: 13); and (iii) portion of the AAVS l region occupying base pairs 405-426: 5' - GCTCGCTCGCTCGCTGGGCGGG - 3' (SEQ ID NO: 14) In the right junction, the breakpoint (represented by the vertical line in SEQ ID NO 12 in Figure 12C) with respect to the genomic DNA is situated 2 bp 3' of the third "GCTC" repeat defining the Rep binding site (.see SEQ ID NO 14 in Figure 12C) As indicated above, the Rep binding site occupies base pairs 405-416 of the AAVSl region With respect to plasmid sequences, this breakpoint involves a region of the lacZ gene that bears no similarity to a Rep binding site In addition, the sequence of the left junction was compared to the nucleic acid sequences of p2TRlacZ and a portion of genomic DNA The nucleic acid sequences are set forth in the bottom left-hand portion of Figure 12C and are as follows (i) left junction 5' - CCTGAGCCACGCGCAGCTGCATTAA - 3' (SEQ ID NO 15), (ii) P 2TRlacZ 5' - GAGCG AGCGAGCGCGCAGCTGC - 3' (SEQ ID NO 16), and (iii) portion of genomic DNA which flanks the site of integration 5' - CCTGAGCCA - 3' In the left junction, the breakpoint with respect to the genomic DNA is represented by the vertical line in SEQ ID NO 15 in Figure 12C Referring to the left junction, the breakpoint in the plasmid is adjacent to a Rep binding site present in the outboard "a" portion of the ITR (corresponding to a' in Figure 4) The genomic DNA at this junction is of unknown origin The first 19 bp of genomic DNA 5' of the left junction are identical to an internal portion of Alu repeats [See E J Mange and A P Mange, Basic Human Genetics, pp. 270-73, Sinauer Associates, Inc (1994)] No homology to the remainder of the Alu repeat or to other DNA was detected in the sequence more distal to the left junction The sequence analysis results physically demonstrate the integration of p2TRlacZ into the AAVS 1 region The results provide confirmation of the data obtained by fluorescent //; situ hybridization showing that the p2TRlacZ integration site is on the q arm of chromosome 19 Thus, novel AAV derived vector systems for gene delivery and integration are disclosed. Although preferred embodiments of the subject invention have been described in some detail, it is understood that obvious variations can be made without departing from the spirit and the scope of the invention as defined by the appended claims SEQUENCE LISTING (1) GENERAL INFORMATION: (l) APPLICANT: NATSOULIS, GEORGES URTZMAN, GARY (ll) TITLE OF INVENTION: ADENO-ASSOCIATED DERIVED VECTOR SYSTEMS FOR GENE DELIVERY AND INTEGRATION INTO TARGET CELLS (m) NUMBER OF SEQUENCES: 16 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: MEDLEN - CARROLL <B) STREET: 220 MONTGOMERY STREET, SUITE 2200 (C) CITY: SAN FRANCISCO (D) STATE: CALIFORNIA (E) COUNTRY: UNITED STATES OF AMERICA (F) ZIP: 94104 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: PCT/US95/ (B) FILING DATE: 15-DEC-1995 (C) CLASSIFICATION: (vn) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: US 08/525,835 (B) FILING DATE: 08-SEP-1995 (vn) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: US 08/357,503 (B) FILING DATE: 16-DEC-1994 (vm) ATTORNEY/AGENT INFORMATION: (A) NAME: CARROLL, PETER G. (B) REGISTRATION NUMBER: 32,837 (C) REFERENCE/DOCKET NUMBER: AVIGEN-02025 (lx) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (415) 705-8410 (B) TELEFAX: (415) 397-8338 (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: ACTTTGAGCT CTACTGGCTT C 21 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: Single (D) TOPOLOGY: linear (11) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2 : GGAGGATCCG CTCAGAGG 18 (2) INFORMATION FOR SEQ ID NO: 3: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3 : CGGGGAGGAT CCGCTCAGAG GTACA 25 (2) INFORMATION FOR SEQ ID NO: : (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION- SEQ ID NO:4- AGGAACCCCT AGTGATGGAG T 21 (2) INFORMATION FOR SEQ ID NO:5 : (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: Single (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5 : CGGCCTCAGT GAGCGAGCGC GC 22 (2) INFORMATION FOR SEQ ID NO:6 : (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: CGCGTTCAAA CCTCCCGCTT CAAAATG 27 (2) INFORMATION FOR SEQ ID NO:7 : (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 80 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7 : GCGGCCGCAC GCGTACGTAC CGGTTCGAAG CGCGCACGGC CGACCATGGT TAACTCCGGA 60 CACGTGCGGA CCGCGGCCGC 80 (2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8 : AAAATTCGAA CAGGTAAGCG CCCCTTTG 28 (2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: AAAATTCGAA CCTGGGGAGA AACCAGAG 28 (2) INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 14 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: GAAOGCGCGC CTTC 14 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 12 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: TTGCGGCCGC AA 12 (2) INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (u) MOLECULE TYPE: DNA (genomic) (Xl) SEQUENCE DESCRIPTION: SEQ ID NO: 12: GCCAGTCTGG GCGGG 15 (2) INFORMATION FOR SEQ ID NO: 13: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 18 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ( i) SEQUENCE DESCRIPTION: SEQ ID NO: 13: GCCAGTCAGG CTTTCTTT 18 (2) INFORMATION FOR SEQ ID NO: 14: ( ) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: GCTCGCTCGC TCGCTGGGCG GG 22 (2) INFORMATION FOR SEQ ID NO: 15: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15- CCTGAGCCAC GCGCAGCTGC ATTAA 25 (2) INFORMATION FOR SEQ ID NO: 16: (l) SEQUENCE CHARACTERISTICS: (A) LENGTH: 22 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (n) MOLECULE TYPE: DNA (genomic) (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16: GAGCGAGCGA GCGCGCAGCT GC 22

Claims: CLAIMS 1. A method of integrating a selected nucleotide sequence into the genome of a mammalian cell, comprising: (a) providing: (i) a first nucleic acid construct comprising a nucleotide sequence flanked by a 5' and a 3' adeno-associated virus inverted terminal repeat, and (ii) a second nucleic acid construct having a rep coding region operably linked to control elements capable of directing the transcription and translation of the rep coding region in said mammalian cell; (b) expressing the rep coding region of said second nucleic acid construct, thereby producing an amount of rep expression product capable of facilitating the integration of said nucleotide sequence of said first nucleic acid construct; and (c) integrating said nucleotide sequence of said first nucleic acid construct into said mammalian cell, said integration being facilitated by said rep expression product. 2 The method of Claim 1 , wherein said first nucleic acid construct is a recombinant adeno-associated virus vector. 3. The method of Claim 2, wherein said recombinant adeno-associated virus vector is a plasmid. 4. The method of Claim 3, wherein said mammalian cell is transfected with said plasmid. 5. The method of Claim 1, wherein said first nucleic acid construct and said second nucleic acid construct are present on the same vector. 6 The method of Claim 1, wherein said first nucleic acid construct and said second nucleic acid construct are present on different vectors. 7 The method of Claim 1, wherein said nucleotide sequence of said first nucleic acid construct is integrated into a chromosome of said mammalian cell 8 The method of Claim 7, wherein said chromosome is chromosome 19q 9 The method of Claim 1, wherein said nucleotide sequence of said first nucleic acid construct is more than 5,000 base pairs 10 The method of Claim 9, wherein said nucleotide sequence of said first nucleic acid construct is more than 10,000 base pairs 1 1 The method of Claim 1, wherein said nucleotide sequence of said first nucleic acid construct encodes a polypeptide 12 The method of Claim 1 1, wherein said polypeptide encodes at least a portion of the factor VIII gene 13 A method of integrating a selected nucleotide sequence into the genome of a mammalian cell, comprising (a) providing (i) a first nucleic acid construct comprising a nucleotide sequence flanked by a 5' and a 3' adeno-associated virus inverted terminal repeat, and (n) an amount of rep expression product capable of facilitating the integration of said nucleotide sequence of said first nucleic acid construct, and (b) integrating said nucleotide sequence of said first nucleic acid construct into said mammalian cell, said integration being facilitated by said rep expression product 14 The method of Claim 13, wherein said first nucleic acid construct is a recombinant adeno-associated virus vector 15 The method of Claim 14, wherein said recombinant adeno-associated virus vector is a plasmid 16 The method of Claim 15, wherein said mammalian cell is transfected with said plasmid 17 The method of Claim 13, wherein said nucleotide sequence of said first nucleic acid construct is integrated into a chromosome of said mammalian cell 18 The method of Claim 17, wherein said chromosome is chromosome 19q 19. The method of Claim 13, wherein said nucleotide sequence of said first nucleic acid construct is more than 5,000 base pairs 20 The method of Claim 19, wherein said nucleotide sequence of said first nucleic acid construct is more than 10,000 base pairs 21 The method of Claim 13, wherein said nucleotide sequence encodes a polypeptide 22 The method of Claim 21 , wherein said polypeptide encodes at least a portion of the factor VIII gene 23 A mammalian cell, comprising (a) a first nucleic acid construct comprising a nucleotide sequence flanked by a 5' and a 3' adeno-associated virus inverted terminal repeat; and (b) a second nucleic acid construct having a rep coding region operably linked to control elements capable of directing the transcription and translation of the rep coding region in said mammalian cell to produce an amount of rep expression product capable of facilitating the integration of said nucleotide sequence of said first nucleic acid construct into the genome of said mammalian cell 24 The mammalian cell of Claim 23, wherein said first nucleic acid construct is a recombinant adeno-associated virus vector. 25 The mammalian cell of Claim 24, wherein said recombinant adeno-associated virus vector is a plasmid 26 The mammalian cell of Claim 25, wherein said plasmid is transfected into said mammalian cell 27 The mammalian cell of Claim 23, wherein said first nucleic acid construct and said second nucleic acid construct are present on the same vector 28 The mammalian cell of Claim 23, wherein said first nucleic acid construct and said second nucleic acid construct are present on different vectors 29 The mammalian cell of Claim 23, wherein said nucleotide sequence of said first nucleic acid construct is integrated into a chromosome of said mammalian cell 30 The mammalian cell of Claim 29, wherein said chromosome is chromosome 19q 31 The mammalian cell of Claim 23, wherein said nucleotide sequence of said first nucleic acid construct is more than 5,000 base pairs 32 The mammalian cell of Claim 31, wherein said nucleotide sequence of said first nucleic acid construct is more than 10,000 base pairs 33 The mammalian cell of Claim 23, wherein said nucleotide sequence of said first nucleic acid construct encodes a polypeptide 34 The mammalian cell of Claim 33, wherein said polypeptide encodes at least a portion of the factor VIII gene 35 A mammalian cell, comprising (a) a first nucleic acid construct comprising a nucleotide sequence flanked by a 5' and a 3' adeno-associated virus inverted terminal repeat, and (b) an amount of rep expression product capable of facilitating the integration of said nucleotide sequence of said first nucleic acid construct into the genome of said mammalian cell 36 The mammalian cell of Claim 35, wherein said first nucleic acid construct is a recombinant adeno-associated virus vector 37 The mammalian cell of Claim 36, wherein said recombinant adeno-associated virus vector is a plasmid 38 The mammalian cell of Claim 37, wherein said plasmid is transfected into said mammalian cell 39 The mammalian cell of Claim 35, wherein said nucleotide sequence of said first nucleic acid construct is integrated into a chromosome of said mammalian cell 40 The mammalian cell of Claim 39, wherein said chromosome is chromosome 19q 41 The mammalian cell of Claim 35, wherein said nucleotide sequence of said first nucleic acid construct is more than 5,000 base pairs 42 The mammalian cell of Claim 41 , wherein said nucleotide sequence of said first nucleic acid construct is more than 10,000 base pairs 43 The mammalian cell of Claim 35, wherein said nucleotide sequence of said first nucleic acid construct encodes a polypeptide 44 The mammalian cell of Claim 43, wherein said polypeptide encodes at least a portion of the factor VIII gene

Inventors: KURTZMAN GARY, NATSOULIS GEORGES, KURTZMAN, GARY, NATSOULIS, GEORGES
Applicants: AVIGEN INC, AVIGEN INCORPORATED
Application year: 1995
Main classification: C12N5
IPC codes: C07K14, C12N5, C12N15