METHODS AND APPARATUS FOR MAKING LIPOSOMES CONTAINING HYDROPHOBIC DRUGS

Abstract: The present application features methods and apparatus for making liposomes containing hydrophobic drugs, with critical, supercritical or near critical fluids. The methods and apparatus combine a phospholipid, a drug, and an aqueous phase, or multilamellar vesicles, with a critical, supercritical or near critical fluid. Upon a reduction in pressure, liposomes are formed.

Description: METHODS AND APPARATUS FOR MAKING LIPOSOMES CONTAINING HYDROPHQBTΓ DRUGS This application is a continuation-in-part of a U.S. Patent Application Serial No. s 08/069, 134, riled May 28, 1993, entitled METHODS AND APPARATUS FOR MAKING LIPOSOMES Field of Invention This invention relates generally to methods and apparatus for making liposomes which o liposomes contain hydrophobic drugs such as taxoids, camptothecins, doxorubicin, michellamine B, vincristine, bryostatin-1, haloman, and cisplatin. The methods and apparatus feature critical, supercritical, or near critical fluids. Background of the Invention s Liposomes are microscopic vesicles having single or multiple phospholipid bilayers which can entrap hydrophilic compounds within their aqueous cores. Hydrophilic compounds may partition into the phospholipid bilayers. Liposomes have been formed in sizes as small as tens of .Angstroms to as large as a few microns. Most liposomes are non-toxic, non-antigenic and biodegradable in character since they have the molecular characteristics of mammalian o membranes. Liposomes are used as carriers for drugs. Liposomes can be made with different features which can enhance a drug's efficacy; reduce a drug's toxicity; and prolong the drug's therapeutic effect. Liposomes with multiple bilayers are known as multilamellar vesicles (MLVs). MLVs 5 are excellent candidates for time release drugs because the fluids entrapped between layers are only released as each membrane degrades. Liposomes with a single bilayer are known as unilamellar vesicles (UV). UVs may be made extremely small (SUVs) or large (LUVs). Liposomes are prepared in the laboratory by sonication, detergent dialysis, ethanol injection, French press extrusion, ether infusion, .and reverse phase evaporation. These methods o often leave residuals such as detergents or organics with the final liposome. From a production standpoint, it is clearly preferable to utilize procedures which do not use organic solvents since these materials must be subsequently removed. Some of the methods impose harsh or extreme conditions which can result in the denaturation of the phospholipid raw material and encapsulated drugs. These methods are not readil scalable for mass production of large volumes of liposomes. Several methods exist for producing MLVs, LUVs and SUVs without the use of organic 5 solvents. MLVs, free of organic solvents, are usually prepared by agitating lipids in the presence of water. The MLVs are then subjected to several cycles of freeze-thawing in order to increase the trapping efficiencies for water soluble drugs. MLVs are also used as the starting materials for LUV and SUV production. One approach of creating LUVs, free of organic solvents, involves the high pressure ι o extrusion of MLVs through polycarbonate filters of controlled pore size. SUVs can be produced from MLVs by sonication, French press or high pressure homogenization techniques. High pressure homogenization has certain limitations. High pressure homogenization is useful only for the formation of SUVs. In addition, high pressure homogenization may create excessively high temperatures. Extremely high pressures are associated with equipment failures. High pressure is homogenization does not insure end-product sterility. High pressure homogenization is associated with poor operability because of valve plugging and poor solution recycling. The use of liposomes for the delivery and controlled release of therapeutic drugs requires relatively large supplies of liposomes suitable for in vivo use. Ostro, M.J. and Cullis, P.R., "Use of Liposomes as Injectable Drug Delivery Systems," American Journal of Hospital Pharmacy. 20 46: 1576-1587 (1989). Present laboratory scale methods lack reproducibility, in terms of quantity an quality of encapsulated drug, lipid content and integrity, and liposome size distribution and captured volume. The multidimensional characteristics of the drug and the liposome, as well as potential raw material variability, influence reproducibility. Present liposome products are not stable. It is desirable to have final formulations which ar 25 stable for six months to two years at room temperature or at refrigeration temperature. Stability requirements have been relaxed by techniques for dehydrating liposomes. Dehydrated liposomes can be distributed to hospitals, free of drugs, and mixed with the drug immediately prior to use by a hospital pharmacist. However, compounding of the liposome containing drug by a pharmacist increases the cost of the therapy and adds further potential for compounding errors. 30 Present liposome products are difficult to sterilize. Sterility is currently accomplished by independently sterilizing the component parts—lipid, buffer, drug and water— by autoclave or filtratio and then mixing in a sterile environment. This sterilization process is difficult, time consuming and expensive since the product must be demonstratively sterile after several processing steps. Heat sterilization of the finished product is not possible since heating liposomes does irreparable damage to liposomes. Filtration through 0.22 micron filters may also alter the features of 5 multilayered liposomes. Gamma ray treatment, not commonly used in the pharmaceutical industry, may disrupt liposome membranes. Picosecond laser sterilization is still experimental and has not yet been applied to the sterilization of any commercial pharmaceutical. Liposomes have been used as a drug delivery vehicle for some hydrophobic drugs. Examples of hydrophobic drugs are taxoids, camptothecins, doxorubicin, michellamine β, vincristine, ICT and cisplatin. The term "taxoid" is used to refer to paclitaxol, cephalomannine, baccatin III, 10-deacetyl baccatin III, deacetylpaclitaxol and deacetyl-7-epipaclitaxol and derivatives and precursors thereof. Paclitaxol is one example of a taxoid. Paclitaxol, also known as TAXOL™, (NSC 125973) is a diterpene plant product derived from the western yew Taxus brevifolia The formula for taxol is set forth below: 15 (a) Paclitaxol 25 This drug, currently in clinical trials, has exhibited a striking 30 to 40% response rate against advanced cases of ovarian and a number of other cancers. Currently, paclitaxol is extracted 30 with organic solvents from the milled bark of T. brevifolia This drug is in short supply. There exists a need to maximize the therapeutic response to this drug to bring the benefits of paclitaxol therapy to as many individuals as possible. It is also necessary to minimize the adverse effect of this drug due to its natural toxic ID. Cephalomannine is another taxoid, cephalomannine is also utilized for the treatment of cancers. Cephalomannine has the formula set forth below: (b) Cephalomannine Camptothecin is derived from a tree, Camptothecin acuminata As used herein, the term "camptothecins" refers to camptothecin and its methoxylated analogs and carboxylated analogs and precursors which have similar biological activity and hydrophobic properties. Camptothecin is also utilized for the treatment of cancers. The formula for camptothecin is set forth below: (c) Camptothecin (CPT) Like paclitaxol, camptothecin is water insoluble and in short supply. The sodium salt of camptothecin has been utilized in initial clinical studies. Recent clinical trials suggest that several camptothecin analogs may have activity against a broad range of human/animal tumors. This composition is believed to act as an inhibitor the enzyme topoisomerase I. Due to the toxicity and 5 hydrophobic nature of this compound, certain adverse side effects have been observed. These adverse side effects include neutropenia, nausea, vomiting, and diarrhea. There exists a need for large scale cost effective manufacturing processes for making hydrophobic drug containing liposomes, which can meet the growing market demand. There exists a need for a process and equipment capable of continuous processing, and capable of recycling ιo unentrapped drugs, lipids and solvents. There exists a need for a process and equipment which produce uniform liposome products. Summary of the Invention The present invention features methods and apparatus for producing liposome containing i s hydrophobic drugs selected from the group consisting essentially of taxoids, camptothecins, doxorubicin, michellamine B, vincristine, bryostatin-1, haloman, and cisplatin. The methods and apparatus are suitable for large scale production of pharmaceutical grade liposomes which are sterile, of a predetermined size, and are substantially free of organic solvents. The present invention features several different methods of making liposomes using critical, 0 supercritical or near critical fluids. One method comprises the steps of forming a solution or mixture of a phospholipid, a hydrophobic drug, an aqueous phase and a critical, supercritical or near critical fluid. The solution or mixture is decompressed to separate the critical, supercritical or near critical fluid, from the phospholipid and aqueous media, to form one or more liposomes. 25 This method is referred to as the decompression method of forming liposomes in several examples. Preferably, the rate of depressurization influences the size of the liposomes formed As used herein, the term "phospholipid" refers to compositions which are esters of fatty acids in which the alcohol component of the molecule contains a phosphate group as an integral part Phospholipids comprise the glycerophosphatides, containing glycerol, and the sphingomyelins 30 containing sphingosine. Preferred phospholipids comprise phosphetidylcholine, phosphatidylethanolamine, phosphatidylserine and sphingomyelin; and synthetic phospholipids comprising dimyristoyl phosphatidyl-choline, dipalmitoryl, phosphatidylcholine, distearoyl, phosphatidylcholine, distearoyl, phosphatidyl glycerol, dipalmitoryl phosphatidylglycerol, dimyristoyl phosphatidyl serine, distearoyl phosphatidyl serine, and dipalmitoryl serine. In the field of physical chemistry, the term "critical fluid" refers to a gas at or above its 5 critical temperatures and at or above its critical pressures. The term "supercritical fluid" refers to a gas above its critical temperature and above its critical pressure. Supercritical fluids are sometimes designated in this application by the abbreviation "SCF." The term "near critical" is used in the sense of approaching or close to being critical. One example, without limitation, of a near critical fluid is a gas having a temperature below its critical temperature and a pressure at or above the critical ιo pressure. Such gas has properties which, may approach those of a supercritical or critical fluid, particularly in solvating properties. In industrial settings where critical, supercritical and near critical fluids are used, it is common, particularly where the solvent properties are being applied, to use the term "critical" to refer to supercritical, critical and near critical fluids. This application will use the term "SCoCoNC i5 fluid" to represent supercritical, critical or near critical fluids. The use of the term "critical" with respect to liposomes and liposome formation refers to liposomes formed with supercritical fluid and near critical fluids as well as critical fluid. Fluids are sometimes referred to in the examples as "critical" as a convenience, even though such fluids may be supercritical, critical or near critical. Solvating properties of SCoCoNC fluids are influenced by cosolvents and entrainers The 20 terms "cosolvents" and "entrainers" are used interchangeably to suggest compositions which are soluble in the SCoCoNC and impart desirable solubility features to the SCoCoNC to which they are added with respect to phospholipids and aqueous phases. Nonpolar cosolvents refer to compositions having an absence of a dipole moment or slight dipole moment, ranging approximately from 0.0 to 0.1 Debyes. Polar cosolvents refer to compositions having a dipole moment, ranging approximately 25 from 0.1 to 1.7 Debyes. As used herein, the term "aqueous phase" refers to a composition comprising in whole, or in part, water. Preferably, the SCoCoNC fluid is selected from the group of compositions capable of forming critical fluids comprising carbon dioxide; nitrous oxide; halo-hydrocarbons, such as freons; 30 alkanes such as propane and ethane; and alkanes, such as ethylene. The "drug" is used in the sense of a therapeutic agent, a chemical or drug capable of effecting a desirable response in an individual subject. The term "hydrophobic" is used in the sense of exhibiting poor solubility in water and strong solubility in hydrocarbon solutions. Preferably, a mixture of the aqueous phase and a solution of the phospholipid in a SCoCoNC fluid is held in a chamber of a first vessel. The solution or mixture is then decompressed as the solution passes to a second chamber or a second vessel. The second chamber allows the SCoCoNC fluid to be removed from the liposome compositions formed, at a temperature and pressure which is different from he first chamber. Preferably the SCoCoNC fluid is recycled. To the extent that phospholipids and aqueous phase are carried over with the SCoCoNC fluid, such components may also be recycled. For convenience, liposomes formed with SCoCoNC fluid are referred to as "critical fluid liposomes" or "CFLs." One embodiment of the present invention features an apparatus for forming liposomes containing a hydrophobic drug in accordance with the decompression method of forming liposome. The apparatus comprises a first vessel wherein one or more phospholipids, an aqueous phase, one or more hydrophobic drugs and a SCoCoNC fluid are combined to form a mixture or solution The hydrophobic drug is selected from the group consisting essentially of taxoids, camptothecins, doxorubicin, michellamine B, vincristine, bryostatin-1, haloman and cisplatin. The apparatus further comprises a second vessel in communication with the first vessel. The apparatus further comprises depressurization means capable of reducing the pressure of the solution or mixture. Depressurization means may be interposed between the first and second vessels or may be integral with the second vessel. The second vessel receives the solution or mixture of phospholipids, an aqueous phase and the hydrophobio drug, which form liposomes upon depressurization. Preferably, SCoCoNC fluid is removed from depressurization means and/or the second vessel and recycled. One embodiment of the present invention featuring a method of forming liposomes, which method features an injection step. This method is referred to in several examples as the injection method of forming liposomes. This method comprises the steps of forming a solution or mixture of a hydrophobic drug, a phospholipid and a SCoCoNC. The hydrophobic drug is selected from the - group consisting essentially of taxoids, camptothecins, doxorubicin, michellamine B, vincristine, bryo statin- 1., haloman, and cisplatin. The solution or mixture is then injected through a tip or orifice into an aqueous phase to form one or more liposomes containing such drug. At the time of injection or thereafter the solution or mixture is decompressed. As a result of the decompression, the SCoCoNC fluid is separated from the phospholipids and the aqueous phase to form liposomes. The released SCoCoNC is either vented or recycled. A preferred method uses a SCoCoNC fluid selected from the group of compositions capabl of forming a critical fluid comprising carbon dioxide; nitrous oxide; halo-hydrocarbons, such as freon; alkanes such as propane and ethane; and alkanes such as ethylene. A further embodiment of the present invention features an apparatus for forming liposomes in accordance with the above method. The apparatus comprises a first vessel for containing a solution or mixture of a hydrophobic drug, a phospholipid and a SCoCoNC fluid. The hydrophobic drug is selected from the group consisting of taxoids, camptothecins, doxorubicin, michellamine B, vincristine, bryostatin-1, haloman, and cisplatin. The apparatus further comprises a second vessel for containing an aqueous phase. The first vessel and the second vessel are in communication by means of injection means for injecting the mixture into the aqueous phase. Upon injection into the aqueous phase and decompression, liposomes containing a hydrophobic drug are formed. Preferably, SCoCoNC fluid is released from the phospholipid upon injection and decompression into the aqueous phase. Preferably, the SCoCoNC fluid is recycled to the first vessel to form additional solutions or mixtures. A further embodiment of the present invention features a method of making liposomes comprising forming a mixture of multilamellar vesicles and a SCoCoNC fluid. The multilamellar vesicles contain a hydrophobic drug. The mixture is decompressed to remove the SCoCoNC fluid to form one or more liposomes of a predetermined size. The size of the liposome can be controlled by the rate of decompression. Preferably, multilamellar vesicles are made by hydrating a mixture of a hydrophobic drug and a phospholipid in an aqueous phase. One embodiment of the present invention features an apparatus for forming liposomes from multilamellar vesicles. The apparatus comprises a first vessel for containing a mixture of multilamellar vesicles and a SCoCoNC fluid. The first vessel is in communication with a second vessel which second vessel is capable of decompressing the mixture to remove the SCoCoNC fluid. During decompression, one or more liposomes are formed. One embodiment of the present invention further comprises a third vessel for forming multilamellar vesicles by hydrating phospholipids in an aqueous phase. The phospholipid contains a hydrophobic drug. An embodiment of the present invention further features control means for determining the rate of decompression. The rate of decompression determines the size of liposomes. Preferably, SCoCoNC fluid removed from the liposome preparation in the decompression vessel is recycled to the first vessel to form additional mixtures of multilamellar vesicles and SCoCoNC fluid. Contact with SCoCoNC fluid may cause destruction of the cellular structures particularly upon rapid decompression. Thus, embodiments of the present invention are, for the most part, self-sterilizing. Methods and apparatus of the present invention are capable of forming liposomes which carry a hydrophobic drug. These drugs can be efficiently placed in liposomes with little loss The liposome vehicle maximizes the therapeutic response to these drugs and minimize toxicities related to the biological activities of the compounds and the hydrophobic physical properties. The hydrophobic drug is incorporated into ingredients which are used to form the liposome. Liposomes made in accordance with the present invention have a narrow size distribution. That is, liposome containing hydrophotsic drug can be made in which 80-100% of the liposomes have a size which is 10% of the average size of the total liposome population. A preferred size for liposomes is between 50-350 nm in diameter. Liposome preparation having a size distribution in which the average size is 100-300 nm, and more preferably 150-290 nm, may incorporate hydrophobic drugs more efficiently. Liposome preparations having a size distribution in which the average size is 150-250 nm may exhibit greater stability Embodiments of the present invention allow the recovery of raw materials, lipids and solvents which are not incorporated into the final liposome product. Embodiments of the present invention feature efficient drug entrapment and recovery of unencapsulated drugs. The operating parameters of the apparatus and method are consistent with other industrially applied processes. The method and apparatus are capable of operating continuously. These and other advantages will be apparent to individuals skilled in the art in view of the drawings and detailed description which follow. -10- Brief Description of the Drawings Fig. 1 schematically depicts an apparatus embodying features of the present invention; Fig. 2 is an exploded view of a nozzle assembly; Fig. 3(a) and (b) graphically depicts a particle size analysis of liposomes formed with SCoCoNC fluid carbon dioxide with two nozzle sizes, (a) 0.5 mm and (b) of 0.06 mm; Fig. 4(a) and (b) graphically depicts a particle size analysis of liposomes formed with SCoCoNC fluid nitrous oxide and ethanol with two nozzle sizes, (a) 6.0 mm and (b) 0.22 mm; Fig. 5 is a bar graph illustrating the effect of pressure on liposome size; and, Fig. 6 is a bar graph illustrating the effect of critical fluid type on liposome size. Fig. 7 depicts a HPLC chromatogram of paclitaxol and cephalomannine. Fig. 8 depicts a HPLC chromatogram of camptothecin at a concentration of 0.04 mg/ml Fig. 9 depicts graphically, in bar graph form, critical fluid liposomal size distribution and intensity of liposomal solution eluted from Gel Exclusion Chromatography (GEC) column. Fig. 10 depicts graphically the concentration profile of paclitaxol and cephalomannine elute from Gel Exclusion Chromatography (GEC) column. Fig 1 1 depicts graphically in bar graph form, the liposome size distribution and intensity distribution of a liposomal solution eluted from GEC column, which liposomes are prepared by sonication. Fig 12 depicts graphically the concentration profile of paclitaxol and cephalomannine containing liposomes from GEC column, which liposomes are prepared by sonication. Fig. 13 depicts a HPLC chromatogram of liposomal encapsulated camptothecin Figs. 14a and 14b depicts paclitaxol concentration as a function of time for a liposome preparation with Fig. 4a representing storage at 4oC, and Fig. 14b representing storage at room temperature (R.T.). Figs. 15a and 15b depicts paclitaxol concentration profile as a function of time for a liposome preparation with Fig. 15a representing storage at 4oC and Fig. 15b depicting storage at room temperature (R.T.). Fig. 16 depicts the toxicity profile of cells to which liposomes made in accordance with the present invention but without paclitaxol were applied as a control study. Fig. 17 depicts the toxicity profile of cells to which paclitaxol was applied, as a percent cell survival, as a function of dosage level. Fig. 18 depicts the toxicity profile of cells to which liposomes made in accordance with the present invention containing paclitaxol, as a percent cell survival as a function of dosage level. Fig. 19 depicts graphically, in bar graph form, a comparison of in vitro percent specific efifect of l .Oμg/ml liposomes containing paclitaxol made in accordance with the present invention and 5 cremophor formulated paclitaxol (CP) for three breast cancer cell lines. Fig. 20 depicts the antitumor activities of LEP, CP (at concentrations of 0.295 mg/ml paclitaxol), and empty liposome (EL) on breast carcinoma cell line, MDX-MB-231, xenografted into the nude mice. o Detailed Description of the Drawings The present invention will be described in detail as a method and apparatus for forming liposomes. The method and apparatus have applications for drug delivery, pharmaceutics, cosmetics, and food processing. One embodiment of the present invention is depicted in Figure 1. An apparatus for making s liposomes, generally designated by the numeral 1 1 is comprised of the following major elements: a first vessel 15; a second vessel 17; a source of SCoCoNC fluid, generally designated by numeral 19, and a low pressure trap assembly, generally designated by the numeral 21. First vessel 15 is adapted to receive a supply of one or more of the following articles or compositions; phospholipids, multilamellar vesicles (MLVs), aqueous phases, SCoCoNC fluids, and o therapeutic agents. In one embodiment, first vessel 15 is capable of receiving phospholipids and an aqueous phase. First vessel 15 is in communication with the source of SCoCoNC fluid 19 to receive SCoCoNC fluid, via conduit 25. The term "communication" is used in the sense of being connected to allow fluid to be directed into or out of a vessel, conduit or the like, or to be in contact with. 5 Conduit 25 is in communication with compressor 27 and storage vessel 29. Storage vessel 29 contains SCoCoNC fluid, which fluid is compelled through conduit 25 by compressor 27. Flow of SCoCoNC fluid through conduit 25 is controlled by valves 25a and 25b. Pressure in conduit 25 is monitored by pressure valve 31. First vessel 15 receives SCoCoNC fluid from conduit 25, which SCoCoNC fluid forms a o mixture with phospholipids and an aqueous phase. First vessel 15 is in communication with second vessel 17 via conduit 33. Back pressure regulator 33a controls pressure in conduit 33. Back pressure regulator 33a reduces pressure on mixtures flowing through conduit 33, which are received by second vessel 17. I one embodiment, conduit 33 terminates in a nozzle 33b within second vessel 17. Second vessel 17 is in communication with exit conduit 35. Valve 35a controls the flow of fluid through conduit 35. Valve 37a controls the flow of fluid in conduit 37. Liposomes which collect in second vessel 17 are withdrawn through exit conduit 35. Second vessel 17 is in communication with trap assembly 21. Trap assembly 21 is comprised of two major elements: first trap vessel 41 and second trap vessel 43. First trap vessel 41 is in communication with second vessel 17 via conduit 45. Back pressure regulator 45a controls the pressure in conduit 45. Flow through conduit 45 is controlled by valve 45a. Pressure in conduit 45 is monitored by pressure meter 47. First trap vessel 41 receives SCoCoNC fluid, and any entrained phospholipids, aqueous phase and liposomes from second vessel 17. Liposomes, phospholipids and aqueous phase are removed from first trap vessel 41 via conduit 49 Valve 49a controls movement of fluids through conduit 49. Shunt 51 is in communication with conduit 49 and conduit 45. Shunt 51 allows sterilizing and cleaning reagents to be pumped through conduit 49 and back flushed in trap vessel 41. Valve 51a controls the flow of fluids in conduit 51. First trap vessel 41 communicates with second trap vessel 43 via conduit 55. Second trap vessel 43 provides a second vessel to receive SCoCoNC fluid and any entrained liposomes, phospholipids and aqueous phase, as mixtures flowing from second chamber 17 and first trap vessel 41 during depressurization. Second trap vessel 43 is maintained in ice bath vessel 57. Ice bath vessel 57 is packed with ice to control and maintain the temperature within second trap vessel 43. Conduit 59, in communication with second trap vessel 43 allows liposomes and unincorporated phospholipids and aqueous phase to be withdrawn Valve 59a controls the flow of fluids in conduit 59. Second trap vessel 43 is in communication with the atmosphere by conduit 63. Flow through conduit 63 is controlled by valve 63a. Flow through conduit 63 is monitored by flow meter 65. First vessel 15 is in communication with a phospholipid mixing assembly, generally designated by the numeral 67. Phospholipid mixing assembly 67 is comprised of the following major elements: a solids vessel 69, a circulation pump 71, a static in-line mixer 73 and three-way valve 75. Solids vessel 69 is adapted to receive hydrophobic drugs, and phospholipids, in solid form, to be solubilized by SCoCoNC fluid. Preferably, the hydrophobic drugs are in a powdered form. Solids vessel 69 receives SCoCoNC fluid from conduit 77. Conduit 77 is in communication with circulation pump 71. Circulation pump 71 is in communication with three-way valve 75 via conduit 79. Valve 79a controls fluid movement in conduit 79. Three-way valve 75 is in communication with static in-line mixer 73 via conduit 81 Static in-line mixer 73 is in communication with solids vessel 69 via conduit 83. Solids vessel 69, circulation pump 71, static in-line mixer 73 and three-way valve 75, of phospholipid mixing assembly 67, define a fluid circuit. The phospholipid mixing assembly 67 is in communication with SCoCoNC fluid storage vessel 29 via conduit 25 which joins conduit 81 between three-way valve 75 and static in-line mixer 73 to allow fluid to be diverted into first chamber 15 and phospholipid mixing assembly 67. Phospholipid mixing assembly 67 has a vent 89. Valve 89a controls the movement of fluid in vent 89. Phospholipid mixing assembly 67 has a drain 91 in communication with conduit 77. Valve 91a controls the movement of fluid through drain 91. Phospholipid mixing assembly 67 has an injection port 93, in communication with three-way valve 75. Injection port 93 allows materials to be injected into first vessel 15 via conduit 122, and into the phospholipid mixing assembly 67. Phospholipid mixing assembly 67 has a pressure meter 95 in communication with conduit 83. Pressure meter 95 allows the pressure in conduit 83 to be monitored. First vessel 15, second vessel 17, low pressure trap assembly 21 and phospholipid mixing assembly 67 are in communication with a wash assembly, generally by the numeral 97. Wash assembly 97 is comprised of the following major elements; a source of air 99, a source of water 101, a source of sodium hydroxide 103, a source of hypochlorite solution 105, and a source of methanol 107. Conduit 109 is in communication with each source via conduit branches 1 1 la-e. Conduit 109 is in communication with conduit 25 to allow the wash reagents to enter the first vessel 15, second vessel 17, low pressure trap assembly 21, and phospholipid mixing assembly 67. Wash reagents, such as water, sodium hydroxide solution, methanol and hypochlorite solutions, are propelled through conduit 109 by pump 1 13. The flow of fluids in wash assembly 97 is controlled by valves 115a, b, c, d, e, f, j and h. The first chamber 15 second chamber 17, phospholipid mixing assembly 67, first trap vessel 41 of low pressure assembly 21 , and connecting conduits are housed in box 1 17. Box 1 17 is heated by heater 119. Heater 119 is controlled by temperature sensor 121b located in conduit 89. Temperature is also sensed by temperature controllers 121a and 121c respectively located on the outside of first chamber 15, and on the outside of second chamber 17. In operation, cleaning solvents are supplied by pump 113. Pump 113 is a close coupled gear pump rated for 6,000 ml/min against a 100 psig head. The cleaning solvents contained in vessels 101, 103, 105, and 107 included 0.1 N NaOH, 10 vol. % hypochlorite solvent, and 95% methanol and deionized water. Deionized water is provided as a flush solvent at a rate of 1,200 ml/min. Instrument compressed air contained in vessel 99 (100 SCFM @ 100 psig) is used as a displacement and drying solvent. The system is periodically cleaned by circulating five system volumes each of hypochlorite solution to inactivate any microorganisms present; deionized water, as a flush; caustic, to remove proteins; deionized water, as a flush; methanol, to solubilize lipids; and deionized water, as a flush The system is blow-dried with compressed air. The apparatus is cleaned between runs by recirculating and then exhausting methanol through the apparatus 11, rinsing the second chamber 17 and low pressure trap assembly 21 with water and then drying with compressed air. Following cleaning, the apparatus 1 1 is dried and brought to operating temperature. All valves are placed in a closed position. In its normal operating mode, the solids vessel 69 is first removed from the apparatus, loaded with a known quantity of phosphatidyl choline (PC)/phosphatidyl ethanolamine (PE) mixture and one or more hydrophobic drugs, and then placed back online in the phospholipid mixing assembly 69. Three way valve 75 is then turned to place injection port 93 in communication with conduit 79. Valve 79a and vent valve 89a are opened. An optional volume of cosolvent or entrainer, such as ethanol, is then introduced via injection port 93 by means of a hypodermic syringe (not shown). Three way valve 95 is then turned to bring phospholipid mixing assembly 67 in communication with first vessel 15, and the vent valve 89a is closed. Valve 25a is then opened, supplying the SCoCoNC fluid solvent to compressor 27. Compressor 27 is turned on and immediately thereafter valve 25b is opened. introducing the SCoCoNC fluid into first vessel 15 and phospholipid mixing assembly 67. When operating pressure is attained, compressor 27 is turned off and valve 25b is closed. After system stabilization, pump 71 is turned on and its speed adjusted. With valve 79a opened, pump 71 sucks both the cosolvent from the bottom of the first vessel 15 and the SCoCoNC fluid phase from the top of the first vessel 15. The mixture is then pumped counter-clockwise, mixed by static in-line mixer 73 and directed by three way valve 75 to first vessel 15. In most cases, an aqueous phase (either deionized distilled water or a buffered solution such as 150 mM saline phosphate buffer at pH - 7.0), is introduced by a hypodermic syringe into the second vessel 17 via sample port 35 and valve 35a. In the alternative, an aqueous phase may be introduced into first vessel 15 to form a mixture of the aqueous phase and phospholipid dissolved in a SCoCoNC fluid. As a further alternative, MLVs are introduced into first vessel 15 to form a mixture of a SCoCoNC and MLVs. Solution and mixture are introduced with sufficient lead time prior to decompression to allow the solution or mixture to achieve the same temperature as the first vessel 15 and the phospholipid mixing assembly 67. After mixing pump 71 is turned off, valve 45b, back pressure regulator 45a, valve 63a are fully opened Back pressure regulator 33a is slowly opened to depressurize first vessel 15 and phospholipid mixing assembly 67. Product is obtained from the second vessel 17, first trap vessel 41 , second trap vessel 43 via conduit 35, 49, and 59, respectively. The volume of each collected sample is measured and recorded. Typically, 95% to 100% of the feed (aqueous and cosolvent phases) is recovered in the first trap vessel 41 and none in second trap vessel 43. The collected samples are stored at 4°C. The liposome preparations made in accordance with the present invention have a narrow size distribution that is 80-100% of the liposomes of a preparation average size of the population. Liposome preparation can be formed in which the size is between 50-350 nm in diameter. Liposomes having a diameter of between 100-300 nm may incorporate hydrophobic drugs more efficiently than smaller liposomes. Liposomes having a diameter of 150-250 nm may exhibit greater stability than smaller liposomes. Other features of the present method and apparatus are exemplified in the following Examples. -16- EXAMΓLE I IMPACT OF NOZZLE SIZE AND DESIGN ON THE CRITICAL FLUID FORMATION OF LIPOSOMES 5 Critical fluid liposomes were formed by first solvating phospholipids in SCoCoNC fluid with/without .an entrainer to form a mixture. The mixture was slowly decompressed into an aqueous phase. The number, size and characteristics of critical fluid liposomes formed are governed by a number of parameters such as the size and design of the decompression nozzle, decompressive ιo forces, critical fluid density (temperature and pressure), interfacial forces, charge distribution, and the characteristics of the encapsulated product and the buffer solution. The impact of nozzle size on the critical fluid formation of liposomes in distilled, deionized (DDI) water is listed in Table 1, and show in Figures 3(a) and (b). Figure 3 graphically depicts particle diameter distribution of liposomes formed by SCoCoNC fluid carbon dioxide with two different nozzles Figure 3(a) depicts the i distribution of liposomes formed with a nozzle having an interior diameter of 0.50 mm and Figure 3(b) depicts the distribution of liposomes formed with a nozzle having an interior diameter of 0.06 mm 20 EFFECT OF NOZZLE SIZE ON LIPOSOME DIMENSIONS (SCF CO2 @ 4,000 psig and 60oC) Exp.No SCF Nozzle Particle Size Analysis (mm) Sm Md Lg (nm) % (nm) % (nm) % LIP- 14 CO2 0.50 0 0 478 100 0 0 LIP- 15 CO2 0.06 0 0 326 100 0 0 30 The liposomes formed with a nozzle diameter of 0.5 millimeters (mm) were readily visible by phase contrast microscopy. Liposomes formed with supercritical fluid carbon dioxide at 4,000 psig and 60oC, had an average size of 478 nanometers (nm). The particle size 35 analysis was done by a size distribution processor (SDP) in a Coulter N4MD laser-based instrument. The SDP allows multimodal size analysis and reports the relative proportion of particles in each discrete size class. The single liposome population had a standard deviation (S.D.) of 180 nm and a 37% coefficient of variance (C.V.). Liposomes formed with a 0.06 mm ID nozzle were smaller and more uniform, having an average particle size of 326 nm, (a S D. of 150 nm and a C.V. of 44%). Based on the data in Table 1, the liposome radius appears to depend on nozzle radius to the one fifth power: R' 2 = R'ι * (rΛ) 1 5 (i) where R' is the radius of the liposome formed, r is the inner radius of the tip of the decompression nozzle. In order to further evaluate the impact of nozzle radius on the size of critical fluid liposomes (CFLs), the nozzle design was changed to incorporate a 0.22 micron (um) filter. Turning now to Figure 2, the filter nozzle assembly, generally designated by the numeral 21 1 is comprised of the following major elements: a housing 213, comprising a male body 215 and a female body 217; and a filter membrane 219. The filter membrane was retained in housing 213, at the end of conduit 33. Male body 215 and female body 217 fit together by cooperating interfitting threaded sections to form a unitary housing 213. The filter membrane 219 was an inorganic membrane with very uniform and non-tortuous pores (Alltech Associates, Inc., Deerfield, JL). The filter membrane 219 was supported by a 316 SS mesh screen 221 and sealed with Teflon O-rings 223. The sizes of liposomes formed by supercritical fluid N 2 O through the 0.06 mm needle tip and the 0.22 micron filter paper are listed in Table 2 and shown in Figure 4. Figure 4 graphically depicts particle size distribution of liposomes formed with SCoCoNC fluid nitrous oxide and ethanol with two different nozzle sizes. Figure 4(a) depicts the distribution of liposomes formed with a nozzle of 0.06 mm and Figure 4(b) depicts the distribution of liposomes formed with a nozzle of 0.22 micron. Table 2 EFFECT OF NOaZZLE DESIGN AND SIZE ON LIPOSOME DIMENSIONS (SCF N 2 O with Ethanol @ 3,000 psig and 60oQ Exp.No. SCF No.zzIe Particle Size Analysis Sm Md Lg (nm) % (nm) % (nm) % LIP-53 N20/EtOH 0.06 mm 0 0 312 100 0 0 LIP-69 N20/EtOH 0.22 urn 0 105 50 389 50 0 In accordance with Equation 1, LIP-53 liposomes should have been reduced in size from 312 nm to 102 nm in LIP-69. There is at least a 50% agreement between these two experiments and Equation 1 in that the 0.22 micron filter reduced the 0.06 mm critical fluid liposomes by at least 50% to 105 nm. A 100% size reduction may not have been possible since the 0.22 micron filter has multiple point exits which could allow neighboring bubbles to agglomerate into larger ones Most of the remaining experiments, unless noted, were conducted with the 0.06 mm orifice EXAMPLE 2 IMPACT OF PRESSURE ON THE CRITICAL FLUID FORMATION OF LIPOSOMES The effect of critical fluid pressure on the size of liposomes formed by the injection technique is listed in Table 3 and shown as a bar chart in Figure 5. As illustrated in Figure 5, for each pressure, the left bar, with lines extending from bottom left to upper right, depicts medium size liposomes (100-400 mm). The right bar, with lines extending from bottom right to upper left, depicts large size liposomes (greater than 400 mm). These CFLs were all formed with a 0.06 mm decompression nozzle. EFFECT OF INITIAL CRITICAL FLUID PRESSURE ON LIPOSOME DIMENSIONS (Critical Fluid CO2 @ 60<>C and 60 mins) Exp. No. Pressure Particle Size Analysis (P«g) Sir 1 Md Lg (nm) % (nm) % (nm) % LIP-36 2,000 0 0 215 50 464 50 LIP-37 2,500 0 0 215 78 464 22 LIP-38 3,000 0 0 352 100 0 0 LIP-48 3,500 0 0 298 18 1150 82 LIP-41 4,500 0 0 372 66 21 10 34 LIP-47 4,500 0 0 317 37 1620 63 LIP-44 5,000 0 0 310 10 1680 90 The increase in liposome size with initial decompression pressure is in agreement with the relationship of bubble growth formation pressure. This relationship is, however, complicated by the amount of phospholipids solubilized in the critical fluid phase and the rate of decompression. The former is significant since the most uniformly sized liposomes (100% at 352 nm) were obtained at 3,000 psig, the optimum pressure for solubilizing lecithin in supercritical fluid carbon dioxide at 60°C. The experiments listed in Table 3 were conducted by circulating the critical fluid for 60 minutes and then slowly decompressing from the listed pressure to atmospheric conditions The liposomes were thus formed from a variable pressure ranging from an initial pressure of 5,000 psig to 0 psig in LIP-37. In order to evaluate the effect of a varying decompression pressure, a series of experiments were conducted in which the liposomes were formed over specific pressure intervals. The results of these experiments are listed in Table 4 below. lalϊ i EFFECT OF FRACTIONAL CRITICAL FLUID DEPRESSURIZATION ON LIPOSOME DIMENSIONS 5 Exp. No Pressure Particle Size Analysis (psig) Sm Md Lg (nm) % (nm) % (nm) % ι o (Critical Fluid N 2 O with Ethanol @ 60C and 60 mins) Lff-64 3,000-2,000 0 0 244 100 0 0 LIP-65 2,000-1,000 0 0 295 100 0 0 LIP-66 1,100-0 0 0 337 56 0 0 15 (Critical Fluid C 2 H 4 with Ethanol @ 60C and 60 mins) LIP- 104 3,000-2,000 0 0 165 100 0 0 LIP-105 2,000-1000 0 0 183 100 0 0 LIP- 106 1,000-0 0 0 140 76 978 24 20 (Critical Fluid C 3 H, with Ethanol @ 60C and 60 mins) LIP-1 10 3,000-2,000 0 0 120 62 1430 38 LIP-1 1 1 2,000- 1,000 0 0 184 28 3160 72 LIP- 112 1,000-0 0 0 136 24 3600 76 25 For example in LIP-64, SCF N2O with a polar cosolvent at 3,000 psig and 60C was contacted with egg yolk lecithin for 60 minutes and then slowly decompressed into DDI water from 3,000 to 2,000 psig and the liposomal solution removed and replaced with fresh DDI water In Lff-65, liposomes were formed by slowly decompressing the remaining critical 3o fluid mixture from 2,000 psig to 1 , 100 psig Finally, in LIP-66, the critical fluid mixture is decompressed from 1 ,100 psig to atmospheric conditions. It should be noted that equal volumes of aqueous phases were used in each of the three stages of decompression. The particle size analyses indicate that a unimodal, relatively 35 small distribution of liposomes was formed at pressures above the critical pressure of N 2 0 which is 1,040 psig. A significant fraction of larger liposomes are formed in decompressing from 1,000 psig to atmospheric conditions. Similar fractional decompression effects on liposome size are shown in Table 4 for ethylene/ethanol and propane/ethanol mixtures. It should be noted that the fractional nitrous oxide/ethanol decompression series was conducted with chicken egg yolk lecithin and a 0.06 mm decompression nozzle; the remaining decompression series in Table 4 were conducted with pure phosphatidyl choline in ethanol with a 0.5 mm decompression nozzle. The larger liposomes in the last stage of fractional decompression are probably formed because the density of the critical fluid changes rapidly below the critical pressure. Operationally, decompression takes much longer at pressures around and below the critical pressure in order to retain the DDI water or aqueous buffer solution in the decompression chamber; also, the discharge volume of gas increases dramatically. This rapid increase in gas volume probably results in the formation of larger bubbles and liposomes because of Joule-Thompson cooling effects due to gas expansion. The LIP- 1 12 sample listed in Table 4 was in fact frozen after the third stage of propane/ethanol fractional decompression EXAMPLE 3 IMPACT OF CRITICAL FLUID TYPE ON THE CRITICAL FLUID FORMATION OF LIPOSOMES Liposomes formed by several critical fluids are characterized in terms of particle size distributions in Table 5 and compared by bar charts in Figure 6. As illustrated in Figure 6, for each critical fluid, the left bar, with lines extending upward from bottom left to upper right, reflect small to medium sized liposomes. The right bar, with lines extending upward from bottom right to upper left, reflect large liposomes. These experiments were all conducted by contacting chicken egg yolk lecithin with the critical fluid phase (without cosolvents) at 3,000 psig and 60°C for 60 minutes, and then slowly decompressing through the 0.06 mm decompression nozzle. lah i EFFECT OF CRITICAL FLUID TYPE ON LIPOSOME DIMENSIONS (Critical Fluid @ 3,000 psig and 60oC for 60 mins) Exp.No. SCF Particle Size Analysis Sm Md g (nm) % (nm) % (nm) % LIP-51 N 2 O 0 0 233 60 4370 40 LP-61 Fr-23 0 0 143 39 705 61 Lff-38 CO 2 0 0 352 100 0 0 LIP-62 CA 0 0 152 21 980 79 LIP-63 C 2 H 4 0 0 320 100 0 0 LIP-60 Fr-22 106 59 348 41 0 0 LIP-55 C 3 Hg 57 82 0 0 1050 18 LIP-56 C 3 Hg 57 82 0 0 1 100 18 Supercritical ethylene in LIP-63 created a unimodal albeit broad distribution (an average particle size of 320 nm and a S D. of 300 nm) of liposomes. Decompression at subcntical pressures could have resulted in bimodal distributions for the remaining critical fluids tested. EXAMPLE 4 IMPACT OF POLAR ENTRAINER OR COSOLVENT ON THE CRITIC . FLUID FORMATION OF LIPOSOMES In general, polar entrainers control the size and uniformity of critical fluid liposomes as shown in Table 6 below: As¬ iatics EFFECT OF POLAR COSOLVENTS ON NITROUS OXIDE CFLs (SCF N2O @ 3,000 psig and 60oC for 60 mins) Exp.No. Cosolvent Particle Size Analysis Sm Md Lg (nm) % (nm) % (nm) % LIP-51 None 0 0 233 60 4370 40 LJP-53 Ethanol 0 0 312 0 0 0 LIP-52 Methanol 88 52 338 42 0 0 LIP-54 Acetone 91 47 311 53 0 0 The micron sized liposome population in LIP-51, SCF N 2 O without a polar cosolvent, was most probably formed during decompression below nitrous oxide's critical point. The addition of 2 vol% ethanol in LIP-53 produces a narrow, unimodal distribution of liposomes having an average size of 312 nm, a standard deviation of 54 nm and a 17% coefficient of variance. Likewise, 2 vol% methanol in LIP-52 and 2 vol% acetone in LIP-54 caused the elimination of the micron sized liposome population. These two additives did, however, form distributions with average sizes around 100 nm and 300 nm. Both distributions are relatively narrow for SCF N 2 O with these two polar cosolvents. The added polar entrainers are most likely controlling the size of the nitrous oxide CFLs by lowering the interfacial tension between the nitrous oxide and water (5% ethanol in water reduces surface tension from 72 dynes/cm at 25°C to 53 dynes/cm; 5% acetone in water reduces the surface tension to 56 dynes/cm). A low, more uniform interfacial tension will control the size of the bubbles and the liposomes formed. The enhanced solubility of lecithin in SCF N 2 O with polar entrainers could also be responsible for the reduction in the sizes and distributions of the CFLs. Polar entrainers have a similar impact on CFLs formed by near critical propane as suggested in Table 7. The addition of a 2 vol% ethanol cosolvent eliminates the micron sized liposome population formed with near critical propane, and creates a single liposome population having an average size of 196 nm and a standard deviation of 300 nm. This elimination and size reduction is probably caused by the alteration of propane- water interfacial properties since, lecithin is very soluble in near critical propane by itself. The sizes of CFLs do also depend on the buffers used and the proteins to be encapsulated. Acetone has a very dramatic impact on propane CFLs, reducing the liposomes to a single population with an average size of 85 nm and a standard deviation of 83 nm. It is quite possible that methanol did not exhibit a similar impact because of the presence of salt (0.09 M NaCl) in LIP-76. Table 7 EFFECT OF POLAR COSOLVENTS ON PROPANE CFLs (Near Critical C 3 Hj @ 3,000 psig and 60oC for 60 mins) Exp.No. Cosolvent Particle Size : Analysis Sm Md Lg (nm) % (nm) % (nm) % LIP-56 None 57 82 0 0 1100 18 LIP-59 Ethanol 0 0 196 0 0 0 Lff-76* Methanol 62 24 0 0 5720 76 LIP-77** Acetone 85 100 0 0 0 0 * Liposomes formed in a saline phosphate buffer with cytochrome-C. ** Liposomes formed in a phosphate buffer with cytochrome-C. There appears to be little or no impact of polar cosolvents on liposomes formed by Freon-22 as shown in Table 8. It should, however, be noted that these three experiments were conducted at different pressures~LIP-60 at 3,000 psig, LIP-73 at 4,000 psig and LIP-75 at 5,000 psig. Initial pressure may have a significant influence on liposome size and distribution. ftbj£_fi EFFECT OF POLAR COSOLVENTS ON FREON-22 CFLs (Near Critical CHC1F2 @ 60oC for 60 mins) Exp.No. Cosolvent Particle Size Analysis Sm Md Lg (nm) % (nm) % (nm) % LIP-60 None 106 59 348 41 0 0 LIP-73 Ethanol 118 76 531 24 0 0 LP-75 Methanol 84 66 473 34 0 0 EXAMPLE S IMPACT OF MODE OF OPERATION ON THE CRITICAL FLUID FORMATION OF LTPOSOMES Size distributions of liposomes formed by the critical fluid injection and decompression techniques are compared and listed in Table 9. Table ? EFFECT OF OPERATIONAL MODE ON CRITICAL FLUID LPOSOMES (C 2 H 4 /EtOH @ 3,000 psig and 60oC for 60 mins) Exp.No. Mode Particle Size Analysis Sm Md Lg (nm) % (nm) % (nm) % LIP-98 Decompression 1 1 87 83 1 1 384 1 LIP-99 Injection 0 0 63 90 1780 10 Both experiments listed in Table 9 were conducted with a 150 mM saline phosphate buffer containing 1 mg/ml cytochrome-C and 9.1 mg/ml of chicken egg yolk lecithin. Also, a slow decompression rate of approximately 1,000 psi min was maintained through a 0.06 mm nozzle tip for both experiments. The data in Table 9 suggests that critical fluid decompression results in a smaller particle size distribution than the critical fluid injection technique. The effect of decompression rate on the size distribution of liposomes formed by the critical fluid decompression technique is compared in Table 10. Both experiments were conducted with identical concentrations of protein and lecithin in a saline phosphate buffer with a 0.50 mm nozzle tip. The data suggests that rapid decompression (approximately 1,000 psi sec) does not significantly impact liposome size; in fact, slow decompression (approximately 1,000 psi/min) appears to offer good control in that a small (mean size of 92 nm), unimodal distribution was obtained in LIP- 100. Table 10 i o EFFECT OF RATE ON LIPOSOMES FORMED BY CRITICAL FLUID DECOMPRESSION (C2H4/EtOH @ 3,000 psig and 60oC for 60 mins) Exp.No. Decompression Particle Size Analysis is Rate Sm Md Lg (nm) % (nm) % (nm) % LIP-9 Rapid 82 87 0 0 2980 13 20 LIP- 100 Slow 92 100 0 0 0 0 EXAMPLE 6 25 ENCAPSULATION CHARACTERISTICS OF LIPOSOMES Encapsulation protocols are typically "passive" in that they rely on the ability of liposomes to capture a certain aqueous volume during vesicle formation. As a result, trapping efficiencies can vary dramatically, ranging from less than 1% for small unilamellar vesicles so (SUVs) to as high as 88% for some multilamellar vesicles (MLVs). Entrapment efficiencies are a function of size and nature of the method (and thus liposome manufacturing technique). Liposomes can also be loaded by relying on their ability to sequester certain drugs in response to transmembrane ion gradients. This "active" protocol, also referred to as remote loading, involves the uptake of charged amphipathic drugs into preformed liposomes which 35 have a transmembrane pH gradient (low intraliposomal pH when the drug involved is ionic) or a transmembrane potential gradient with exogenous ionophores such as potassium ion. For example, trapping efficiencies of 98% and drug:lipid ratios as high as 1 :2.2 (w/w) can be readily achieved for doxorubicin hydrochloride in a LUV system (Mayer et al., 1985). Unlike the "passive" protocol, trapping efficiency is independent of lipid concentration. Transmembrane ion gradients not only accomplish efficient drug encapsulation but also decrease the rate of drug efflux from the vesicles as much as 30-fold. An alternate method of obtaining high trapping efficiencies and high drug: lipid ratios is to chemically attach a hydrophobic group (e.g. a fatty acid or phospholipid) to the drug; this creates a molecule that is highly soluble in the liposome membrane. Hydrophobic drugs can be dissolved in the lipid phase prior to liposome formation. Liposomes made with SCoCoNC fluids can be loaded with a desired composition in any manner which would apply to liposomes made by conventional techniques. The loading of cytochrome-C in liposomes formed by sonication and critical fluids is summarized in Table 11. The loading was passive in the sense that cytochrome-C was present in the aqueous phase during the formation of the liposome. Iab H SIZE AND TRAPPING EFFICIENCIES OF CRITICAL FLUID LIPOSOMES (Critical fluids @ 3,000 psig and 60oC for 60 mins) Exp.No. SCF Lipid Cone Particle Size Analysis Trapping (mg/ml) Efficiency Sm Md Lg (nm) % (nm) % (nm) % LIP-96 Sonication 10.0 1 1 85 97 15 13.5 LJP-97 C2H4/EtOH 9.1 82 87 0 0 2980 13 14.9 LIP-98 C2H4/EtOH 9.1 1 1 87 83 1 1 384 1 12.9 LIP-99 C2H4/EtOH 9.1 63 90 0 0 1780 10 14.0 LIP-85 C3H8/EtOH 16.6 0 0 166 54 998 46 27.7 LIP-87 C3H8/EtOH 3.9 88 17 0 0 3690 83 18.5 LIP-89 N2O/EtOH 3.9 97 33 384 6 3000 61 33.3 EXAMPLE 7 STABILITY OF CRITICAL FLUID LIPOSOMES 5 Stability of critical fluid liposomes depends on a variety of parameters such as raw material composition, purity and oxygen susceptibility, end product sterility, compatibility between encapsulated drug and liposomal materials, aqueous phase pH and ionic strength and composition, and preparation technique. Lack of stability will impact liposome size and drug retention capability. For pharmaceutical applications, liposomal formulations are desirable to l o have a shelf-life between 6 to 24 months at 4°C. Liposomes are subject to massive fusion and leakage during conventional freeze-drying and rehydration. By coating each side of the lipid membrane with equimolar concentrations of sugars such as glucose, free-flowing powdered liposomal preparations can be formed that retain 90% or more of the entrapped materials with no change in size of the is liposomes (Crowe et al., 1985). Stability problems can also be avoided by "remote loading" preformed liposomes at the time of use (Bally et al., 1985). Remote loading can be readily accomplished by changing the pH of a preformed liposomal preparation in order to create a transmembrane pH gradient prior to adding the therapeutic drug in the required dosage. Reproducible and complete uptake of the drug is achieved within five minutes, resulting in an 20 injectable liposomal formulation (Ostro et al., 1989). Stability of liposomal formulations can also be increased by using synthetic saturated lipids or by adding antioxidants such as alpha-tocopherol and Beta-hydroxytoluene to prevent phospholipid degradation. The stability of critical fluid liposomes was examined in order to evaluate if critical fluids enhanced or decreased the stability of liposomal formulations. This examination was 2 5 conducted by measuring the particle size distribution as a function of time. No special precautions, such as preparation of critical fluid liposomes under a blanket of inert gas, the use of antioxidants, or aseptic processing and collecting procedures were utilized in the preparation of critical fluid liposomes. The time stability of critical fluid liposomal formulations are listed in Table 12. 30 Iab I2 IMPACT OF NOZZLE SIZE ON TIME STABILITY OF CRITICAL FLUID LIPOSOMES (SCF CO2 @ 4,000 psig and 60oQ Exp.No. Elapsed Nozzle Particle Size Analysis Time (mm) (days) Sm Md Lg (nm) % (nm) % (nm) % LIP-14 18 0.50 0 0 478 100 0 0 LIP-14 52 0.50 0 0 509 100 0 0 LP-14 187 0.50 0 0 316 41 1810 59 LIP-15 6 0.06 0 0 326 100 0 0 LIP-15 52 0.06 0 0 312 100 0 0 LIP-15 187 0.06 0 0 315 100 0 0 Supercritical carbon dioxide liposomes exhibit good to excellent stability at a storage temperature of 4oC over a six (6) month period as shown by the data in Table 12. The smaller diameter liposomes, formed by the 0.06 mm nozzle, appear to be more stable than the larger liposomes formed by the 0.50 mm nozzle. As a second point of comparison for critical fluid liposomes, the relative stability of liposomes formed by sonic energy can be inferred from the listing in Table 13. ____!____ STABILITY OF LIPOSOMES FORMED BY SONIC ENERGY (10 minutes @ 60oC) Exp.No. Buffer Elapsed Particle Size Analysis Time — . (days) Sm Md Lg (nm) % (nm) % (nm) % LIP-91 DDI 1 22 100 0 0 0 0 LIP-91 DDI 23 28 93 97 7 0 0 LIP-95 PBS 1 61 100 0 0 0 0 LIP-95 PBS 24 40 90 216 10 0 0 Lff-96 PBS/ 1 1 1 85 97 15 0 0 Cytochrome-C LIP-96 PBS/ 24 0 0 109 79 956 21 Cytochrome-C The liposomes formed by sonic energy in deionized distilled (DDI) water exhibit a small amount of agglomeration after twenty three (23) days of storage at 4©C. The time stability of liposomes formed by other critical fluids over a four (4) month period are presented in Table 14. The data indicates that the most effective critical fluids, in order of decreasing stability, were: (1) propane; (2) Freon-22; (3) nitrous oxide; (4) ethane; (5) Freon-23 : and (6) ethylene. Tahle 14 EFFECT OF CRITICAL FLUID TYPE ON TIME STABILITY OF CRITICAL FLUID LPOSOMES. (Critical Fluid @ 3,000 psig and 60oC for 60 mins) Exp.No. SCF Elapsed Intensity Particle Size Analysis Time (days) Sm Md Lg (nm) % (nm) % (nm) % LP-51 N 2 O 1 0 0 233 60 4370 40 LP-51 N,O 124 0 0 189 76 1780 24 LP-61 Fr-23 1 0 0 143 39 705 61 LP-61 Fr-23 1 17 29 81 278 19 0 0 LP-62 C 2 H, 1 0 0 152 21 980 79 LP-62 1 14 0 0 294 100 0 0 LP-63 C.H« 1 0 0 320 100 0 0 LP-63 C 2 H, 1 14 0 0 238 38 10000 62 LP-60 Fr-22 1 106 59 348 41 0 0 LP-60 Fr-22 117 138 73 521 27 0 0 LP-56 C 3 H, 1 57 82 0 0 1 100 18 LP-56 C 3 H j 1 19 50 89 0 0 772 1 1 In general, polar cosolvents improved the stability of critical fluid liposomes This improvement is exemplified in Table 15 which shows that the stability of critical nitrous oxide liposomes is much better with methanol as an additive; both ethanol and acetone additives are better than none and have a similar impact on the stability of N 2 O critical fluid liposomes. Table 15 EFFECT OF POLAR COSOLVENTS ON TIME STABILITY OF CRITICAL FLUID LPOSOMES (SCF N 2 O @ 3,000 psig and 60oC for 60 mins) Exp.No. Co- Elapsed Particle Size Analysis Solvent Time (days) Sm Md Lg (nm) % (nm) % (nm) % LP-51 None 1 0 0 233 60 4370 40 LP-51 None 7 33 49 317 49 4090 22 LP-51 None 124 0 0 189 76 1780 24 LP-53 Ethanol 1 0 0 312 0 0 0 LP-53 Ethanol 7 0 0 297 100 0 0 LP-53 Ethanol 123 98 53 317 47 0 0 LP-52 Methanol 1 88 52 338 42 0 0 LP-52 Methanol 7 88 58 312 42 0 0 LP-52 Methanol 123 97 62 345 38 0 0 LP-54 Acetone 1 91 47 31 1 53 0 0 LP-54 Acetone 7 100 47 332 53 0 0 LP-54 Acetone 123 0 0 31 1 100 0 0 EXAMPLE 8 PACLITAXOL AND CAMPTOTHECIN CONTAINING CFLs OPERATIONAL CONDITIONS The apparatus of the type depicted in Figure 1 was operated at an average pressure of 3,000 - 4,000 psig, average temperature of about 50-60oC, and a circulation time of 60 minutes. Two gases, ethylene and propane, were used in this Example. This examples features paclitaxol and camptothecin with the understanding that other hydrophobic drugs including without limitation, cephalomannine, doxorubicin, michellamine B, vincristine, hydrostatin- 1, haloman and cisplatin would perform similarly. Under the operational conditions propane was a near critical fluid. The critical temperature of propane is 96°C. At the operating conditions for this Example, propane is considered to be a near critical fluid exhibiting solvent properties approaching that of a critical fluid. The thermodynamic properties propane or ethylene are listed in Table 16. Table 16 Relevant thermodynamic properties of critical fluids. Critical Fluid Formula T c (°CI P c (psigl r c (g/cc) Dipole Moment Ethylene C2H 9.2 731 0.217 0.0 Propane C3HR 96 5 616 0.217 0.084 Phospholipids Raw Materials Fresh frozen chicken egg yolk (Sigma Chemical Co., St Louis, MO), which consisted of 60% phosphatidyl choline (PC) and 16.5% phosphatidyl ethanolamine (PE), soy bean phosphatidylcholine (>95% purity) (Avanti Polar Lipids, Inc., Alabaster, AL) were used as liposomal raw materials. Pure cholesterol (>99% purity) was also used. Taxol and Camptothecin Raw Materials Taxol was obtained by extraction from raw materials. Camptothecin was obtained from the National Cancer Institute (NCI), Washington, DC. A mixture of paclitaxol and cephalomannine was obtained from the NCI. HPLC analysis of this mixture showed that the weight percentage of paclitaxol and cephalomannine in the mixture were 20% and 30%, respectively. Analytical Methods Liposomes formed by critical fluids and sonication were characterized in terms of size, stability, and efficiency of encapsulation. A submicron particle analyzer (Coulter Electronics, Inc. Model N4MD) was used to measure average particle size, size frequency distribution, and standard deviation of particle size. This computer controlled, multiple-angled instrument utilizes a laser beam to sense light scattering by the particles in its path, due to the particle's Brownian motion and by photon correlation spectroscopy to provide particle size analysis. HPLC analysis for paclitaxol content in liposome formulation was performed on a 15 cm long pentafluorophenyl (PFP) column (ES Industries, Inc.) with a Waters 501 HPLC pump and a Waters 991 phodiode array detector. The flow rate and the temperature were controlled at 1.5 ml min. and 27°C, respectively. The mobile phase consisted of 44% acetonitrile, 56% water, and about 0.1% (vol.) of phosphoric acid. This system gave retention times for paclitaxol and cephalomannine around 12.5 and 9.5 minutes, respectively, with UV detection at 228 nm. The HPLC chromatogram of the paclitaxol/cephalomannine (taxoid) mixture is presented in Figure 7. The camptothecin content of liposomes was assayed by HPLC using a reversed phase 15-cm long pentafluorophenyl (PFP) column. Using a mobile phase consisting of 80% methanol, 20% water, and 0.1% (vol.) phosphoric acid at a flow rate of 1.0 ml/min, camptothecin elutes in about 2.9 minutes. At a wavelength of 370 nm, chromatographic scan of the camptothecin solution at a concentration of 0.04 mg/ml is shown in Figure 8. Gel-exclusion chromatography (GEC) was used to separate unencapsulated taxoid from liposomes, and to determine the loading efficiency of taxoid in the liposomes. A 16 mm ID x 70 cm long column packed with Sephacryl S-1000 (Pharmacia LKB Biotechnology, Piscataway, NJ) with an exclusion diameter of 260 to 300 nm was used to fractionate liposomes and separate liposomes from any unencapsulated solute. The packed GEC column was saturated with liposomal solution. This step is necessary in order to prevent subsequent liposomal formulations from sticking to the Sephacryl S 1000 beads. The eluted fractions were collected by a fraction collector (ISCO, Inc. Lincoln, NE). The particle size analysis and taxoid content of eluted solution were respectively analyzed by the Coulter particle analyzer, and by HPLC Centrifugation was applied to liposomes at 10,000 g for 15 minutes followed by filtration through a 0.22 mm filter. HPLC analysis for paclitaxol was performed before and after the filtration. This relatively simple method would give an indication of whether paclitaxol is incorporated into the liposomes. A. Lipoςomes Formed bv lηiection Liposomes containing paclitaxol or paclitaxol and cephalomannine, or camptothecin were formed as described in Example 1. Such liposomes were made using a 0.5mm nozzle at a pressure of 4,000 psig and a temperature 60©C. Taxol, or paclitaxol and 5 cephalomannine or camptothecin, in powdered form, were placed with phospholipids into a phospholipid chamber 69 of the apparatus depicted in Figure 1. The apparatus was operated to dissolve the phospholipid and drug in SCoNC fluid to form a drug mixture. This mixture is injected into an aqueous phase to form liposomes. ιo B. Liposomes Formed bv Decompression Liposomes containing paclitaxol, or paclitaxol and cephalomannine, or camptothecin were formed as described in Example 1. That is, phospholipid, drug and aqueous phase, and a SCoCoNC fluid are thoroughly mixed in an apparatus as described in Fig. 1. The apparatus is operated at a pressure of 4,000 psig and a temperature of 60°C. This i5 mixture is held in a first vessel and placed in fluid communication with a second vessel via a nozzle. Upon depressurization of the mixture, as the mixture moves from the first vessel to the second, or upon depression of the mixture upon entering the second vessel, liposomes containing drug are formed. 20 C. Liposomes Formed From Multilamellar Vesicles Liposomes containing a hydrophobic drug would be made from multilamellar vesicles by first making a multilamellar vesicle in accordance with any art recognized method. A preferred method would be from a mixture of a phopholipid and the hydrophobic drug. This mixture would be hydrated in an aqueous solution to form multilamellar vesicles. The 25 multilamellar vesicles would be mixed with a SCoCoNC fluid and decompressed to form liposomes of a uniform size. EXAMPLE 9 COMPARISON OF LPOSOME SIZE DISTRIBUTION 30 Size distribution of liposomes formed by the critical fluid injection and decompression techniques, are compared in Table 17. All experimental data listed in Table 17 was conducted with average pressure and temperature around 4,000 psig and 60°C, respectively. The results in Table 17 indicate that the decompression technique resulted in a smaller particle size distribution than that of the injection technique. The difference of size distribution in liposomes formed by using two different operational modes can probably be explained by the mechanism 5 under which liposomes are formed. Table 17 i Effect of operational mode on size distribution of critical fluid liposomes. Exp. Operation Particle Size Analysis Number Mode Small Medium Large (nm) % (nm) % (nm) % UP-154 + Injection 0 0 237 35 2730 65 LIP-169' injection 0 0 157 38 954 62 LIP-152 + Decomp. 97 89 547 11 % 0 0 LIP-170* Decomp. 70.2 92 0 0 1000 8 15 ■ + Propane was used as SCoNC fluid. * Ethvlene was used as SCoNC fluid. Without intending to limit the invention to a particular theory, in the injection technique, phospholipid solvated in the SCoCoNC fluid may deposit out at the phase boundary 2 0 of critical fluid/water bubbles as result of depressurization. While these bubbles rise after detaching from the nozzle into the aqueous solution, the crystallized bilayers of phospholipids peel off, and seal themselves to form liposomes. The size distribution of liposomes will depend on several operating parameters such as: size and design of nozzle, rate of decompression, interfacial tension between the SCoCoNC fluid and aqueous medium, and pressure and 5 temperature in the collection vessel. In this operational mode, the solvation process of phospholipids by critical fluid occurs in the high pressure circulation loop. Consequently, the liposome formation process in the decompression chamber can be operated at a different pressure and temperature environment from that required to solvate the phospholipids. This technique is well suited for the encapsulation of therapeutic proteins and compounds which are o thermally labile. By its nature, the injection technique is also well suited for therapeutic proteins and compounds which are shear sensitive. In the decompression technique, solvated phospholipids in the form of a bilayer in the critical fluid are continuously dispersed into the aqueous solution in the soaking chamber, and consequently vesiculate into liposomes. When the equilibrium stage between the critical fluid and phospholipids is reached during the circulation under constant temperature and pressure, a certain amount of solvated phospholipids will remain in the critical fluid as bilayer fragments, and circulate in the high pressure loop. During the decompression process, the mixture of critical fluid, lipid fragments and aqueous are forced through the dip tube/nozzle under large decompressive forces generated by volume expansion of the critical fluid. Existing liposomes and bilayer fragments of phospholipid may undergo collision with each other due to large shear forces, and are torn apart into smaller fragments of phospholipid. The size of these bilayer fragments depends on the pressure and the rate of decompression. These thermodynamically unstable bilayer fragments will then seal themselves rapidly to form liposomes in the decompression vessel. In order to evaluate the effects of decompression pressure on liposome size distribution, two fractional decompression experiments were conducted. In these experiments, sample solution was collected at two different pressure intervals, the first one from operating pressure, from 3500 psig, to 900 psig, which is slightly above the critical pressures of ethylene and propane, and the second one from 900 psig to atmospheric pressure. The results of these experiments are listed in Table 18. These experiments were conducted under the same operational pressure and temperature, and with the same nozzle at the tip of the decompression tube. Table 18 The effect of fractional depressurization on liposome size distribution. (CF: ethylene; Circulation time: 60 min.). Exp. Decomp. Panicle Size Analysis Number Pressure Interval Small 1 Medium Large (psig) (nm) % (nm) % (n ) % LIP-171 3500 - 900 62 100 0 0 0 0 900 - 0 0 0 1 18 69 992 31 LIP-172 3500 - 900 69 100 0 0 0 0 900 - 0 59 100 0 0 0 0 The results from Table 18 demonstrate that it is possible to obtain liposomes with uniform size distribution by properly selecting the decompression pressure range, and the nozzle size, provided that the other conditions remain the same. EXAMPLE 10 CHARACTERIZATIONS OF LIPOSOME ENCAPSULATED TAXOL The liposomal samples from liposome formation processes featuring injection and decompression were centrifuged at 2,000 rpm for 30 minutes, to remove any unencapsulated solutes and large phospholipid crystals in the liposome suspension. An HPLC assay was performed to determine the paclitaxol and cephalomannine content of liposomes in the supernatant of the centrifuge tube. For comparative reasons, liposomes formed by sonication were also centrifuged and the suspended liposomes were assayed for paclitaxol and cephalomannine content. The results are presented in Table 19 below. Table 19 HPLC assay of Paclitaxel and Cephalomannine concentration (μς/ml) from critical fluid liposomes (-4,000 psig and 60°C for 60 minute*), end sonication liposomes (sonicated at -1Q°C for 30 minutes). Exp. No. Operational Before Centrifu g ation After Ceptrifu atioη TV hange Mode Paclitaxel Cephal. Pa itaxβl Cephal. Paclitaxel Cephal. LIP-149 Decomp. 17.2 24.8 16.9 24.3 0.7) (2.021 LIP-154 Injection 77.6 S0.3 68 86.5 (12.4) (4.2) LIP-1 GG Sonication 85.4 1 28.3 46 73.3 (46.3) (42.9) The data clearly demonstrates that liposomes formed by injection or decompression encapsulate paclitaxol and cephalomannine. The data also suggests that injection or decompression processes capture paclitaxol more effectively than those formed by the sonication method. In LP-166 formed by sonication, it appears that a large amount of unencapsulated paclitaxol was present in the solution, more than 45%, and that untrapped paclitaxol and cephalomannine precipitated out after centrifugation. The incorporation of paclitaxol in liposomes was examined by utilizing Gel Exclusion Chromatography (GEC) to fractionate liposomes in different sizes, and to determine whether the paclitaxol moves with the liposomes. The elutant from the GEC column was analyzed, again, by HPLC. The particle size analysis including particle size distribution of eluted sample was also examined by the Coulter particle size analyzer. GEC chromatography of the encapsulation of paclitaxol by liposomes produced by injection processes are depicted in Figure 5. In Figure 5, it is evident that liposomes were eluted out from the column between 18 and 40 ml. The volume fractions collected under these peaks were cloudy. The particle size intensity (counts sec.) increases dramatically from 1.05 x 10 4 at about 18 ml, to 2.6 x 10 6 at 23 ml, and peaked around 3.0 x 10 6 . The intensity eventually decays to 3.0 x 10* at about 54 ml. The average size of corresponding liposomes eluted from column peaked around 260 nm at 30 ml, and gradually reduced to 31 nm at about 48 ml The first two volume fractions of LP-154 were unimodal, and the remaining three were bimodal with a small portion of liposomes (4-20%) in the 650 to 900 nm range. The particle size analysis for LP-154 which had an original intensity of 2 6 x 10 6 or 3.0 x 10 6 was measured at a 1 : 10 or 1 :20 dilution in sterile filtered water in order to obtain an intensity level within the operational range of instrument It is quite possible that the GEC process causes liposomal agglomeration by bringing small liposomes within their charge repulsion diameters so that van der Waals forces of attraction can take effect. Our experiments also suggest that a certain amount of paclitaxol and cephalomannine incorporated in the bilayer of liposomes may be transferred to the GEC column during size fractionation. HPLC analysis of paclitaxol and cephalomannine for eluted solutions of LP-154 from the GEC column was performed. These data are represented graphically in Figure 10, the concentration of paclitaxol is depicted with an open diamond and the concentration of cephalomannine is depicted with a closed diamond. It is evident that entrapped paclitaxol eluted mostly with liposomes in the approximately 150-240 or 160 to 270 nm size range. The eluted peak at 18 ml has virtually identical concentrations of paclitaxol and cephalomannine, around 3 mg/ml. Both paclitaxol and cephalomannine concentrations increased sharply, and peaked around 33 mg/ml at 35 ml. Data representing the liposome size and intensity distribution from the GEC column is depicted in bar graph form in Figure 9. Data representing size (in nm) is depicted with a bar with dots, data representing intensity (xlO 4 counts per sec), is depicted with a cross hatched line bar. This concentration also corresponds to the highest intensity level of particles shown in Figure 9. Figure 11 and Figure 12 show the analytical results of GEC fractionation of LP-166 prepared by the sonication method (see Table 4). Figure 1 1 depicts, in bar graph form, 5 intensity and size of liposomes prepared by sonication. Size is represented by a bar with dots, and intensity (xlO 4 counts per sec) is represented by crossed hatched line bar, a large amount of liposomes eluted out of the GEC column from 20 to 40 ml, and solutions under these peaks were also cloudy. Entrapped paclitaxol and cephalomannine were eluted with liposomes of around 180 nm from GEC column. These results are depicted in Figure 12 Data representing o paclitaxol is illustrated by an open diamond, and data representing cephalomannine is illustrated with a closed diamond. These results are consistent with results obtained with LP-154 which was prepared by the injection process. However, in LP-166, the amount of paclitaxol and cephalomannine entrapped in the liposomes was much lower, about ten times, than those from the injection process. The majority of particles (86%) in LP-166 formed by sonication are in i5 the 38 nm range, and would not be expected to have a high paclitaxol content since small sized liposomes do not trap paclitaxol as effectively as larger liposomes. Table 20 presents the results of two additional experiments, LP-175 and LP-176, in which paclitaxol was encapsulated in critical fluid liposomes. In these two experiments, cholesterol and egg yolk PC were mixed in a 1 :2 ratio by weight. The fractional decompression 20 technique was used during the depressurization process. Ethylene and propane were utilized as the critical fluid for LP-175 and LP-176, respectively. I_____2L\ Concentration of Paclitaxel and Cephalomannine as well as panlde size distribution 25 in lP-175 and IP-176 Exp. No. Decomp. Paclitaxel Cephal. Partide Size Analysis • Press. Concen. Concen. S all Medium tcflfi Interval (psig) (mg/ml) (mg/ml) (nm) % (nm) % (nm) % LIP- 175 2800 - 900 17.9 31.1 50 100 0 0 0 0 900 - 0 43.2 67.8 0 0 1 17 52 990 48 LIP-1 6 4200 - 800 9.2 13.0 90 100 0 0 0 0 800 - 0 16 9 26 6 0 0 166 69 1020 31 30 A uniform size distribution of liposomes from the first stage of fractional decompression in both experiments was again obtained. However, paclitaxol concentration in this population of liposomes is relatively low. In our size exclusion chromatography study for LP-154 (Figures 9 and 10), it was shown that paclitaxol eluted out mostly with liposomes in the 160 to 270 nm size range. This suggested that an optimal size of liposomes to encapsulate paclitaxol and cephalomannine may be in the range of 150-290 nm. In the second stage of decompression, the particles are distributed into two larger population sizes in both LP-175 and LP-176. This may be due to the rapid volume expansion of critical fluid below its critical pressure which is considerably larger than that in the first decompression stage Liposomes formed in the high pressure circulation loop prior to the decompression go through a rapid disruption and fusion process, and vesiculate into larger liposomes. EXAMPLE 11 CHARACTERIZATIONS OF LIPOSOME ENCAPSULATED CAMPTOTHECIN The samples of liposomal encapsulated camptothecin, prepared by decompression in accordance with Example 1, were centrifuged at 2,000 rpm for about 30 minutes. Particle size distribution was measured on the Coulter N4MD Submicron Particle Analyzer The HPLC analysis was conducted before and after the centrifugation as described in the section of Analytical Method. Figure 13 represents a typical HPLC chromatogram of liposomal encapsulated camptothecin. The same analysis was also performed for liposomes containing camptothecin prepared by sonication where the same formulation of egg PC with cholesterol were dispersed in the aqueous solution. The solution was sonicated with a Branson sonifier probe (Model 450, Branson Ultrasonics Corp. Danbury, CT) for 30 minutes in an ice bath. The results in the Table 21 suggest that decompression processes encapsulated camptothecin more effectively than sonication. In sonication, most camptothecin is deposited out of the aqueous solution after the centrifugation. I_ύ____l HPLC analysis for the concentration of camptothecin (μg/ml) and particle size distribution from CFL process and sonication method Exp. Operational Before Centrifugation After Centrifugation No. Mode Camptothecin. Cone. Camptothecin Cone. % Change 1 Decomp. 22.5 23.0 +2% 2. Sonication 75.6 10.2 -87% Table 22 suggest size of liposomes produced by decompression processes is generally larger than that prepared by the sonication method. Particle size analysis for liposomes encapsulated camptothecin prepared by CFL process and by sonication method Exp. Operational Particle Size Analysis No. Mode (nm) Small Amt(%) Medium Amt(%) Large Amt(%) 1 CFL decomp. 122 64 0 0 4270 36 2 Sonication 15.2 92 402 0.8 5620 7 EXAMPLE 12 STABILITY COMPARISON OF LPOSOME ENCAPSULATED TAXOL USING INJECTION OR DECOMPRESSION PROCESSES AND SONICATION The in vitro stability of liposomes will generally depend on several parameters, such as phospholipid composition and purity, oxygen susceptibility, compatibility between encapsulated drugs and liposomes, and aqueous medium conditions. Many different changes can take place in liposomes with the passage of time. The phospholipids can undergo chemical degradation such as oxidation and hydrolysis. Liposomes maintained in aqueous suspension may aggregate, fuse, or leak their contents. Two samples (10 ml each) from experiments, LP-237 and LP-247, were collected, 5 and stored at 4°C and 22°C (room temperature), respectively. Both experiments utilized identical operational conditions (P=3,000 psi and T β 50°C), and the same phospholipid materials (soy PC and CH). However, in LP-237 run, paclitaxol mixture (20% purity) was used, and in LP-247, pure paclitaxol with >99% purity was used. The initial paclitaxol content in LP-237 and LP-247 was 40 and 133 ppm by HPLC, respectively. HPLC analysis was ιo performed periodically for LP-237 and LP-247 at two different storage conditions. No special precautions, such as preparation of critical fluid liposomes under a blanket of inert gas, use of antioxidants, or aseptic processing and collecting procedures were used in these experiments. Table 23 compares the stability of size distribution analysis of LP-237 and LP-247 i5 prepared by critical fluid injection technique, with LIP- 166, prepared by conventional sonication method, (all stored at 4°C) as a function of time. The date suggests that liposomes formed by injection processes exhibit superior physical stability than those formed by sonication. Liposomes made by sonication, LP-166, had a large amount of small particles, 77% at 38 nm, and these liposomes appeared to fuse and aggregate together to form relatively 2o large size particles in just 10 days. Liposomes made by the injection process (LP-247) remained the same from its initial size even after 40 days. Liposomes formed by injection processing, LP-237, showed signs of aggregation after 35 days; the majority of these liposomes double their size after 60 days. The relative impure taxoid mixture used in this run may have caused this early aggregation. At room temperature storage condition, all liposomal paclitaxol samples showed various degrees of deterioration. Iab 22 Comparison of liposomes size in the samples of LIP-237 and HP247, formed by critical fluid formation process, and of LIP-166, formed by the sonication method. Experimental Operation Elapsed Time Particle Size Number Mode lday(s)] Small Medium Large 30 nm % nm % nm % LIP-237 Injection. 1 152 100 0 0 0 0 Technique 60 0 O 355 100 0 0 LIP-247 Injection 1 218 100 0 0 0 0 Technique 40 213 100 0 0 0 ' 0 LIP- 166 Sonication 1 38.5 86 932 14 0 0 Method 10 80.8 69 846 31 0 0 Figures 14a and 14b illustrate paclitaxol concentration profiles for LP-237 and LP-247, respectively, as a function of time and temperature. Figures 14a and 15a depict samples stored at 4©C, and Figures 14b and 15b depict samples stored at room temperature. The results suggest that at 4°C storage conditions, paclitaxol can remain stable in the liposome suspension for over two months. At room temperature storage condition, deterioration started after two days. These results demonstrate that the injection liposome formation process can produce physically and chemically stable aqueous-based formulations of paclitaxol. Preferably, such liposomes have a size distribution of 150-250 nm and, more preferably, 200-225 nm. EXAMPLE 13 CYTOTOXICITY STUDIES OF LPOSOME ENCAPSULATED TAXOL A. Toxicity of Liposomes Encapsulating Taxol Against Colon Cancer Cell Lines An independent study for cytotoxicity of liposome encapsulated paclitaxol prepared by decompression processes was conducted. Samples of LIP- 175 and LP-176, all prepared by the critical fluid decompression technique were tested against the HCT 1 16 human colon cancer cell line. A sample of LP-170 which contained no paclitaxol or cephalomannine was also tested against the same cell line as a control. In the cytotoxicity studies, a five order of magnitude dilution of the liposomal encapsulated paclitaxol was used. After seeding into the plates for four hours, the cells were treated with liposomes encapsulated paclitaxol and paclitaxol as control in 0.5% DMSO buffer. The liposomes were left on the cells for three days after which time the plates were re-fed and MTT was added. The reduction of the MTT to a purple formazan product correlates in a linear way with the number of living cells in the well plates. Therefore by measuring the absorptance of this reduction product, the percent of cell survival at a given dose of paclitaxol can be quantified. The results of these studies were plotted with percent of cell survival as a function of paclitaxol concentration of the paclitaxol in the liposomes. Results of liposomes formed in accordance with the present methods, without paclitaxol are illustrated in Fig. 16. Results with paclitaxol, without liposomes, are illustrated in Fig. 17. Results of liposomes with paclitaxol are illustrated in Fig. 18. The data suggest that liposomes containing paclitaxol exhibit similar drug activity compared with that of paclitaxol without liposomes. B. Toxicity Evaluation of LEP Against Breast Carcinoma Cell Lines. The effectiveness of liposomal paclitaxol to inhibit growth of three breast carcinoma cells in tissue culture was determined. Three (3) breast cancer cell lines, MCF-3, BT-20 and MDA-MB-231. Said cell lines were grown in tissue culture flasks. Known number of cells were plated in 24-well tissue culture plates and allowed to grow overnight in a tissue culture incubator at 37°C with 5% CO 2 . Cells were then treated with 1.0 μg/ml concentration of liposomal paclitaxol, 1.0 μg/ml cremophor-paclitaxol, and empty liposomes (EL) diluted in cell culture media. A control group of cells were kept untreated. Cell culture media was replenished on the third day following the treatment. The treatment continued for seven (7) days. Cells in each well were counted using trypan blue. Cell viability and cell growth inhibition were calculated from the number of dead versus viable cells, and from the total number of cells grown per well. Percent specific killing for liposomal paclitaxol and cremophor-paclitaxol (CP) against such breast cancer cell lines was calculated based on percentage killed from liposomal paclitaxol and cremophor-paclitaxol data subtracting from the corresponding empty liposome data. The results are illustrated, in bar graph form in Figure 19. Data representing liposomal paclitaxol is illustrated with a solid bar. Data representing cremophor-paclitaxol is illustrated with a shaded bar. The data suggest that, at 1.0 μg/ml, liposomal paclitaxol had a much greater specific in vitro effect than cremophor-paclitaxol. EXAMPLE 14 IN VIVO STUDIES OF LPOSOMAL ENCAPSULATED PACLITAXOL (LEV) The objective of this study was to determine growth inhibition of breast carcinoma xenografts in nude mice by intraperitonealy (i.p.) administration of liposomal encapsulated paclitaxol (LEP). The breast cancer cell line, MDX-MB-231, was chosen based on the results of in vitro studies shown in the EXAMPLE 13. Liposomal paclitaxol from experiment LP-247 was used in this in vivo study. Breast carcinoma xenografts were grown in nude mice. Mice were randomly divided into four groups. Each group consisted of five (5) mice. Tumor size was measured by a caliper and expressed in volume (mm 3 ). Treatment began when tumor reached measurable size, — 100 mm 3 . Liposomal paclitaxol and cremophor-paclitaxol were given at a concentration of 0.295 mg paclitaxol in 0.5 ml, and an equivalent amount of empty liposomes were given for the control group via injection i.p. at two (2) day intervals for a total of five (5) injections. Mean Volume ± Standard Error of the Mean (SEM) were recorded each time when measurements were taken. All mice were sacrificed 4 weeks after the first injection. The xenografts of the nude mice with breast cancer cell lines, MDX-MB-231, after treatment with LEP, liposomal paclitaxol, cremophor-paclitaxol and empty liposome (as a control) and cremophor (as a control) were evaluated. The results, tumor volume versus weeks of treatment, are graphically depicted in Fig. 20. Results from mice receiving liposomal-paclitaxol are plotted with an enclosed rectangle; results from mice receiving cremophor-paclitaxol are plotted with an enclosed triangle; results from mice receiving empty liposomes are plotted with closed circles; and results from mice receiving cremophor alone are plotted with open circles. After five doses of liposomal paclitaxol, cremophor-paclitaxol and empty liposomes, mice receiving liposomal-paclitaxol exhibited better antitumor effect than the cremophor-paclitaxol. Embodiments of the present invention allow the recovery of raw materials, lipids and solvents which are not incorporated into the final liposome product. Embodiments of the present invention feature efficient drug entrapment and recovery of unencapsulated drugs. The operating parameters of the apparatus and method are consistent with other industrially applied processes. The method and apparatus are capable of operating continuously. Thus, while preferred embodiments of the invention have been described, the present invention is capable of variation and modification and, therefore, the present invention should not be limited to the precise details set forth, but should include such changes and alterations as fall within the purview of the following claims. What is claimed is:

Claims: CLATMS 1. A method of making liposomes comprising: a) forming a solution or mixture of a phospholipid, one or more hydrophobic 5 drugs and an aqueous phase in a critical, supercritical or near critical fluid, said drug selected from the group of hydrophobic drugs consisting essentially of taxoids, cephalomannine, camptothecins, doxorubicin, michellamine B, vincristine, bryostatin-1, haloman, and cisplatin, b) reducing the pressure of the solution or mixture to separate the critical, supercritical or near critical fluid from the phospholipid and aqueous phase, said phospholipid ι o and aqueous phase forming one or more liposomes containing said drug. 2. The method of claim 1 wherein said critical, supercritical, or near critical fluid is selected from the group of compositions capable of forming critical fluid comprising carbon dioxide, nitrous oxide, propane, ethylene and ethane. 15 3. The method of claim 1 wherein said mixture or solution is decompressed as the mixture or solution exits a nozzle. 4. The method of claim 8 wherein said nozzle has one or more openings which opening 20 has a diameter ranging from approximately .5 to .6 microns 5. The method of claim 1 wherein said critical, supercritical or near critical fluid comprises entrainers. 25 6. The method of claim 10 wherein said entrainers are selected from the group of compositions consisting of methanol, ethanol and acetone. 7. The claim of claim 4 wherein said that phospholipids are phosphatidylcholine, phosphatidylethanolamine, phosphatidyl serine, and sphingomyelin. 30 8. The method of claim 1 wherein said that phospholipids are synthetic phospholipids consisting of dimyristoyl phosphatidylcholine, dipalmitoryl phosphatidylcholine, distearoyl phosphatidylcholine, distearoyl phosphatidylglycerol, dipalmitoryl phosphatidylglycerol, dimyristoyl phosphatidyl serine, distearoyl phosphatidyl serine, and dipalmitoryl phosphatidyl serine. 9. The method of claim 1 wherein said that phospholipids contain small amount of cholesterol. 10. The method of claim 1 wherein said liposome has a hydrophobic drug incorporated in its lipid bilayer. 1 1. The method of claim 1 wherein said taxoid is paclitaxol 12. The method of claim 1 wherein said camptothecins is camptothecin 13. A method of making liposomes comprising: a) forming a solution or mixture of a phospholipid, a drug and a critical, supercritical or near critical fluid, said drug selected from the group consisting essentially of hydrophobic drugs consisting essentially of taxoids, camptothecins, doxorubicin, michellamine B, vincristine, and cisplatin; b) injecting said solution or mixture into an aqueous phase to form one or more liposomes. 14. The method of claim 13 further comprising the step of decompressing the solution or mixture as said solution or mixture is injected. 15. The method of claim 12 wherein said solution or mixture is injected into said aqueous phase through a nozzle. 16. The method of claim 19 wherein said nozzle has at least one opening having a diameter in the range of approximately .5 to .6 microns. 17. The method of claim 12 wherein said critical, supercritical or near critical fluid is selected from the group of compositions capable of forming critical fluids comprising carbon dioxide, nitrous oxide, halo-hydrocarbons, propane, ethylene, and ethane. 18. The method of claim 12 wherein said critical, supercritical or near critical fluid further comprises entrainers. 0 19. The method of claim 18 wherein said entrainer is selected from the compositions consist of methanol, ethanol, and acetone. 20. The claim of claim 13 wherein said that phospholipids are phosphatidylcholine, s phosphatidylethanolamine, phosphatidyl serine, and sphingomyelin. 21. The method of claim 13 wherein tsaid that phospholipids are synthetic phospholipids which include but not limited to: dimyristoyl phosphatidylcholine, dipalmitoryl phosphatidylcholine, distearoyl phosphatidylcholine, distearoyl phosphatidylglycerol, 0 dipalmitoryl phosphatidylglycerol, dimyristoyl phosphatidyl serine, distearoyl phosphatidyl serine, and dipalmitoryl phosphatidyl serine. 22. The method of claim 13 wherein said that phospholipids contain cholesterol. 5 23. The method of claim 13 wherein said liposome has a hydrophobic drug incorporated in its lipid bilayer. 24. The method of claim 13 wherein said taxoid is paclitaxol or cephalomannine o 25. The method of claim 13 wherein said camptothecin is camptothecin 26. A method of making liposomes comprising: a. forming a mixture of multiple bilayer liposome and a critical, supercritical or near critical fluid, said multiple bilayer liposome having a hydrophobic drug, said drug selected from the group consisting essentially of taxoids, camptothecins, doxorubicin michellamine B, 5 vincristine, bryostatin-1, haloman and cisplatin. b. reducing the pres of the mixture to separate the critical, supercritical or near critical fluid from the mixture to form liposomes of a uniform size containing said drug. 27. As an article of manufacture, a plurality of liposomes containing a hydrophobic drug ι o having a size distribution of 50-350 nm. 28. The article of manufacture of claim 27 wherein said liposomes have a size distribution of 100-300 nm. i5 29. The article of manufacture of claim 27 wherein said liposomes have a size distribution of 150-250 nm.

Inventors: CASTOR TREVOR P, CHU LING, CASTOR, TREVOR, P., CHU, LING
Applicants: APHIOS CORP, CASTOR TREVOR P, CHU LING, APHIOS CORPORATION, CASTOR, TREVOR, P., CHU, LING
Application year: 1995
Main classification: A61K9
IPC codes: A61K9, A61K31, A61K33