MULTILAMELLAR DRUG DELIVERY SYSTEMS

Abstract: A pharmaceutical preparation including a pharmaceutical agent incorporated into particles comprising (i) a core formed from a hydrophilic material, a hydrophobic material or a hydrophobic emulsion or dispersion and (ii) an alternating sequence of hydrophilic/hydrophobic layers thereon such that there is a hydrophilic/hydrophobic interface between the core and each succeeding layer.

Description: MULTILAMELLAR DRUG DELIVERY SYSTEMS The ability of drugs to be administered via the oral route depends on several factors. The drug must be soluble in the gastrointestinal fluids in order for the drug to be transported across biological membranes, or be suitable for an active transport mechanism. Very small particulateε (less than 300 nanometers) can be absorbed through the Peyer's Patch system in the small intestine, and through the lymphatic system, but this mechanism is not capable of absorbing large doses of drugs into the systemic circulation. A problem arises for hard to dissolve drugs. In the case of conventional drugs, some drugs are relatively insoluble in gastrointestinal fluids. If the extent of solubility is low, this may cause incomplete and/or erratic absorption. If the rate of solubility is low, then absorption will most probably be erratic on an intra-patient and inter-patient basis. Peptide drugs can be water soluble, and these are not as problematic as insoluble peptides. Like conventional drugs, insoluble peptides typically exhibit incomplete or low extent of absorption and erratic absorption or bioavailability The primary issue in the ability to deliver peptides orally is the protection of the drug from proteolytic enzymes. There are two basic approaches to do this. The first is an "enteric" coating that can be applied to release the drug only in neutral to basic pH, so that the peptide is not exposed to gastric juices. However, this approach alone is not sufficient to protect the peptide since proteolytic enzymes exist in the upper intestinal tract, and some degradation of the drug can still occur. The other approach is to incorporate the peptide in a hydrophobic material so that aqueous fluids cannot penetrate the system. In this way, the peptide is protected from proteolytic enzymes. In addition, it is possible to combine the two approaches. However, there are inherent difficulties with the approach outlined above. First, many drugs are released too slowly from hydrophobic systems. Also, some peptides will partition into the hydrophobic phase so that they will not be fully released from these systems. Thus, both the rate and extent of drug release are crucial components of any drug delivery system, and are even more important for many peptide drugs. In accordance with the present invention there is provided a pharmaceutical preparation or delivery system including a pharmaceutical agent incorporated into particles comprising (i) a core formed from a hydrophilic material, a hydrophobic material or a hydrophobic emulsion or dispersion and (ii) an alternating sequence of hydrophilic/hydrophobic layers thereon such that there is a hydrophilic/hydrophobic interface between the core and each succeeding layer. The particles include the core or prill seed and multiple alternating hydrophobic and hydrophilic layers, forming solid-state or semi-solid vesicles, in a multilamellar structure. The core can be formed of hydrophilic or hydrophobic materials or of a hydrophobic discontinuous phase emulsified or dispersed in a hydrophilic continuous phase, in which case the surface of the core or seed will take on the hydrophilic character of the continuous phase. The surfactant and the two phases stabilized form an emulsion. This emulsion can take the form of a complex water-in-oil-in- water-etc. emulsion oil-in-water-in-oil-etc. emulsion. Each phase can be very small in size or volume, or can be a discernible coating over the previous phase with a surfactant stabilizing the interface. Hydrophilic and hydrophobic layers are formed onto the core or seed such that there is always an alternating hydrophilic/hydrophobic interface formed. Thus, a core with a hydrophilic surface will first be coated with a hydrophobic layer followed by a hydrophilic layer, and vice versa. Drugs of the type delivered by the delivery system of the invention partition into either or both phases, according to the drugs' partition coefficient and are released from the particles by diffusion and erosion of the vesicle in vivo . The spheres can be delivered by the oral, nasal, otic or inhalation routes of administration and have a size range of from 0.5 to about 100 microns. When the core is formed of an emusion the hydrophobic material forms the discontinuous phase and the hydrophilic material forms the continuous phase in which the hydrophobic material is emulsified. The hydrophobic discontinuous phase and the hydrophilic continuous phase can each independently be solid, semisolid or liquid. The pharmaceutical agent may be dispersed or incorporated into the hydrophobic material, the hydrophilic material or in both the hydrophobic and hydrophilic materials. Preferably the carrier emulsion is a colloid, microcolloidal emulsion or, most preferably, a icroemulsion. An emulsion is a disperse system containing at least two immiscible phases, a hydrophobic phase and a hydrophilic phase. The emulsion comprises the dispersed phase, the dispersion phase and an emulsifying agent or surfactant agent, except when the hydrophobic material is a "self-emulsifying" ester, making a separate emulsifying agent unnecessary. The term "colloidal" refers to emulsions in which the dispersed phase is of very fine particles, usually less than about 1 mm in size. A "microcolloid" is an emulsion wherein the dispersed particles are usually about 100 urn or less in size. Cosurfactants are also common components of microcolloids and are simply surfactants included in addition to the primary surfactant. A "microemulsion" is an optically isotropic and thermodynamically stable liquid emulsions. The emulsions of the invention are generally made by adding hot (70-80° C) hydrophobic phase (smaller by weight) to hot (70-80° C) hydrophilic phase (larger by weight) forcing inversion of the surface active agent to form a disperse emulsion of unaggregated dispersed phase particles. This produces an emulsion when processed under suitable shear. The drug is usually added with the hydrophobic material when it is an organic molecule that is poorly soluble in aqueous media. The drug is usually added after the emulsion has been formed and allowed to cool when it is a peptide. The drug in emulsion formulation is then filled into a soft or hard gelatin capsule, tablet or other oral dosage form. In the multilamellar pharmaceutical preparation of the present invention certain hydrophobic materials provide enhanced absorption capabilities for oral delivery of peptide drugs and drugs that are poorly soluble in aqueous media. In accordance with the invention, these materials are preferably selected from the group consisting of long chain carboxylic acids, long chain carboxylic acid esters, long chain carboxylic acid alcohols and mixtures thereof. Certain long chain carboxylic acid esters are self-emulsifying, that is that a surfactant is not necessary for these compounds to be emulsified into a hydrophilic phase. The long chain carboxylic acids, generally contain from 6-30 carbon atoms and preferably contains at least 12 carbon atoms, most preferably 12 to 22. In some cases this carbon chain is fully saturated and unbranched, while others contain one or more double bonds. A few contain 3-carbon rings or hydroxyl groups. Examples of saturated straight chain acids are n-dodecanoic acid, n- tetradecanoic acid, n-hexadecanoic acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, behenic acid, montanic acid and melissic acid. Also uselful are unsaturated monoolefinic straight chain monocarboxylic acids. Examples of these are oleic acid, gadoleic acid and erucic acid. Also useful are unsaturated (polyolefinic) straight chain " monocaboxyic acids. Examples of these are linoleic acid, linolenic acid, arachidonic acid and behenolic acid. Useful branched acids include, for example, diacetyl tartaric acid. Examples of long chain carboxylic acid esters include, but are not limited to, those from the group of: glyceryl monostearates; glyceryl monopalmitateε; mixtureε of glyceryl monostearate and glyceryl monopalmitate (Myvaplex 600, Eastman Fine Chemical Company); glyceryl monolinoleate; glyceryl monooleate; mixtures of glyceryl monopalmitate, glyceryl monostearate glyceryl monooleate and glyceryl monolinoleate ( yverol 18-92, Eastman Fine Chemical Company); glyceryl onolinolenate; glyceryl monogadoleate; mixtures of glyceryl monopalmitate, glyceryl monostearate, glyceryl monooleate, glyceryl monolinoleate, glyceryl monolinolenate and glyceryl monogadoleate (Myverol 18-99, Eastman Fine Chemical Company); acetylated glycerides such as distilled acetylated onoglycerides (Myvacet 5-07, 7-07 and 9-45, Eastman Fine Chemical Company); mixtures of propylene glycol monoesters, distilled monoglycerides, sodium stearoyl lactylate and silicon dioxide (Myvatex TL, Eastman Fine Chemical Company); mixtures of propylene glycol monoesters, distilled monoglycerides, sodium stearoyl lactylate and silicon dioxide (Myvatex TL, Eastman Fine Chemical Company) d-alpha tocopherol polyethylene glycol 1000 succinate (Vitamin E TPGS, Eastman Chemical Company); mixtures of mono- and di- glyceride esters such as Atmul (Humko Chemical Division of Witco Chemical); calcium stearoyl lactylate; ethoxylated mono- and di-glycerides; lactated mono- and di-glycerides; lactylate carboxylic acid ester of glycerol and propylene glycol; lactylic esters of long chain carboxylic acids; polyglycerol esters of long chain carboyxlic acids, propylene glycol mono- and di-esters of long chain carboxylic acids; sodium stearoyl lactylate; sorbitan monostearate; sorbitan monooleate; other sorbitan esterε of long chain carboxylic acidε; succinylated monoglycerides; εtearyl monoglyceryl citrate; stearyl heptanoate; cetyl esters of waxes; cetearyl octanoate; C, o -C 30 cholesterol/lavosterol esters; and sucrose long chain carboxylic acid esters. Examples of the self-emulsifying long chain carboxylic acid esters include those from the groups of stearates, palmitates, ricinoleates, oleates, behenates, ricinolenates, myristateε, laurateε, caprylateε, and caproates. The alcohols useful in the invention are exemplified by the hydroxyl forms of the carboxylic acids exemplified above and also cetearyl alcohol. The types of protective or sustained release coatings that can be used include, but are not limited to, ethylcellulose, hydroxypropylmethylcellulose, hydroxypropylcellulose, hydroxyethylcellulose and esters of methacrylic and ethacrylic acid (Eudragit RL, RS, and NE polymer products, Rohm Pharma, Darmstadt, Germany). The enteric protective materials or coatings can be, for example, cellulose acetate pthalate, hydroxypropylmethylcellulose pthalate, ethylvinylacetate pthalate, polyvinylacetate pthalate and esters of methacrylic and ethacrylic acid (Eudragit S and Eudragit L, Rohm Pharma, Darmstadt, Ger. ) . The composition or preparation of the invention can further include a surfactant, an amphiphilic molecule conεiεting of a hydrophobic tail and a hydrophilic head. Theεe molecules possess distinct regions of both hydrophilic and hydrophobic character. The hydrophobic tai can be a hydrocarbon or fluorocarbon chain of 8 to 18 carbon atoms. The hydrophilic head can be ammonium, εulfate, εulfonate or carboxylate if ionic, and polyoxyethylene if non-ionic. " They are long chain moleculeε εuch as, for example, soapε or detergentε. Surfactants accumulate at the hydrophilic/hydrophobic (water/oil) interface and lower the surface tension. Surface active agents or surfactant are long chain moleculeε, such as soaps and detergents, which accumulate at the hydrophilic/hydrophobic(water/oil) interface and lower the surface tension. One effect of a reduced surface tension is the stabilization of the emulsions. This is because molecules with both polar and non-polar groups become oriented such that the hydrocarbon tail embeds itself into the hydrophobic phase and the hydrophilic head protrudes into the hydrophilic phase. Where the hydrophobic composition or other component of the preparation includes a surface-active agent, such as a surfactant, it is usually present in amounts of about 0.05% to 10.0% weight/weight of the hydrophobic composition with a preferred range of 0.1% to 2.0% (w/w) . The drugs to be incorporated individually or as combinations in the pharmaceutical preparations of the invention are those having less than about 80% oral bioavailability. The term "bioavailability" as used here means the rate and extent of systemic absorption of a drug from the oral route of administration. In one aspect, the drug is a polypeptide, usually of less than about 15 amino acids. Examples include cyclosporin, angiotensin I, II and III, enkephalins and their analogs, ACTH, antiinflammatory peptides I, II, III, bradykinin, calcitonin, cholecystikinin (CCK) fragments 26-33 and 30-33, pre/pro CCK (V-9-M), J3- endorphin, dinorphin, leucokinin, leutinizing hormone releasing hormone (LHRH), neurokininε (e.g. neurokinin A), somatostatin, substance P, thyroid releasing hormone (TRH) and vasopressin. In another aspect, the drug is an organic molecule that is poorly soluble in aqueous media. These organic moleculeε usually have a molecular weight (m.w. ) of less than about 1,000 daltons, and usually leεε than about 600 daltons. Examples include acyclovir, adriamycin, cabamazepine, griseofulvin, angiotenεin converting enzyme inhibitorε, flutamide, melphalan, nifedipine, indomethacin, naproxen, estrogens, testoεteroneε, steroids, phenytoin, sumatripan, ergotamines and cannabinoidε. In accordance with the invention, drugs are incorporated into hydrophobic materials by admixture using conventional mixing devices and homogenizers used for semi-solid ointments and lotions, with agitation at speeds common to emulsified products such as creams and emulsions. Examples of common equipment employed are propeller or turbine mixers, homogenizers, colloid mills, ultrasonic mixers and icrofluidizerε. The shear of the agitation should be sufficient to form a stable dispersion, but not too great to cause degradation of the drug. Suitable homogenizers are available from Micromedics, Inc., Silverson, APV Crepaco and Arde Barinco. This hydrophobic emulsion or dispersion is then formed into particleε by εpray-congealing or "prilling. " This process uses a spray nozzle which atomizes the material in a cooling tower. As the material is sprayed, surface tension causes a uniform spherical particle to be formed. As the particle falls through the cooling chamber, it hardens into a stable, intact sphere. These spheres can be sprayed in a variety of diameters, generally from 0.5 microns to 100 micronε. It is preferred to reduce the size of the εphere as much as possible, preferably below 5 microns for the primary hydrophobic "seed" prill. These particles are then coated in a fluidized bed apparatus with an alternating sequence of (i) a surfactant-containing hydrophilic solution and (ii) a stable emulsion or dispersion of the drug in a hydrophobic material to provide the multilamellar drug delivery system of the invention. The hydrophilic phase is sprayed on with a binder that adheres to the hydrophobic seed. Then a hydrophobic material is sprayed on which will adhere the aqueous surfactant phase. What follows is a process of repeatedly alternating between these two stepε in a fluid bed apparatuε. Thiε iε done in one fluid bed that haε differential preεεureε that organize the flow in that bed. The seed iε repeatedly coated with the hydrophilic εolution for a set number of minutes and then with the hydrophobic emulsion or dispersion for an independent set number of minutes. The apparatus used is a fluid-bed coating apparatus, such as is available from Wurster and others. The newly coated particles are then coated with the hydrophobic mixture from above, and then alternating hydrophilic and hydrophobic coatings to produce a multilamellar structure. The drug partitions into each phase, according to its partition coefficient, and is released from the syεtem by diffuεion, eroεion, and enhanced diεεolution reεulting from the εurface-active nature of the interfaceε. The particles can be incorporated into hard gelatin capsules, either with additional excipients, or alone. Typical excipients to be added to a capsule formulation include, but are not limited to: fillers such as microcrystalline cellulose, soy polysaccharideε, calcium phosphate dihydrate, calcium sulfate, lactose, sucrose, sorbitol, or any other inert filler. In addition, there can be flow aids such as fumed silicon dioxide, silica gel, magnesium stearate, calcium stearate or any other material imparting flow to powders. Because of their hydrophobic nature, the particles should not need a lubricant, but one can be added if neceεεary by uεing polyethylene glycol, leucine, glyceryl behenate, magnesium stearate or calcium stearate. The particles may also be incorporated into a tablet, in particular by incorporation into a tablet matrix, which rapidly disperses the particles after ingestion. In order to incorporate these particles into such a tablet, a filler/binder must be added to a tablet that can accept the particles, but will not allow their destruction during the tableting process. Materials that are suitable for this purpose include, but are not limited to, microcrystalline cellulose (Avicel), soy polysaccharide (Emcosoy), pre-gelatinized starches (STARCH 1500, National 1551), and polyethylene glycols (Carbowax). The materials should be present in the range of 5-75% (w/w), with a preferred range of 25-50% (w/w) . In addition, disintegrants are added in order to disperεe the particles once the tablet is ingested. Suitable diεintegrants include, but are not limited to: cross-linked sodium carboxymethyl cellulose (Ac-Di-Sol), sodium starch glycolate (Explotab, Primojel), and cross- linked polyvinylpolypyrrolidone (Plasdone-XL) . These materials should be present in the range of 3-15% (w/w) , with a preferred range of 5-10% (w/w) . Lubricants are also added to aεεure proper tableting, and these can include, but are not limited to: magnesium stearate, calcium stearate, stearic acid, polyethylene glycol, leucine, glyceryl behanate, and hydrogenated vegetable oil. These lubricants should be present in amounts from 0.1-10% (w/w), with a preferred range of 0.3-3.0% (w/w). Tablets are formed, for example, as follows. The particles are introduced into a blender along with Avicel, disintegrants and lubricant, mixed for a set number of minutes to provide a homogeneous blend which is then put in the hopper of a tablet press with which tablets are compressed. The co presεion force used is adequate to form a tablet; however, not sufficient to fracture the beads or coatingε. The capεule or tablet can alεo be enteric coated. Either the particleε can be enteric coated (Ph sensitive) and released in the stomach or the capsule or tablet can be enteric coated (thereby releasing the particles in the intestine), in which case the particles need not be so coated. To use only a sustained release coating on the particle one would alεo need an enteric coated capsule or tablet. There are three approaches here. First, there is the uncoated hydrophobic particle in an enteric coated capsule. Second, there is the εuεtained releaεe coated particle within an enteric coated capsule or tablet. Third, there iε the enteric coated particle encloεed within a regular εoft gelatin capεule or uncoated tablet. The capεule may be further proceεεed to provide gaεtric protection by enterically coating the capεule. When the contentε of the capεule are releaεed into the gaεtrointeεtinal milieu, it spontaneouεly forms a microcolliodal emulsion with the gastrointestinal fluid. The gastrointeεtinal fluid acting as the aqueous phase. The preparation of an emulsion based system, requires that the drug be dispered into the hydrophobic material as described above, with the aqueous phase being added in the presence of surfactant or self-emulsifying hydrophobic long chain carboxylic acid ester. This procedure under suitable shear forms a microemulsion. This emulsion is then filled into a soft of hard gelatin capεule. The capεule may be further proceεεed to provide gastric protection by enterically coating the capsule. EXAMPLE 1 Phase Ingredients %W/W B Somatostatin 15 B Polyethylene Glycol 4000 20 B Polyethylene Glycol 8000 20 A Polysorbate 80 5 A Oleic Acid 40 Preparation: To form the emulsion core, mix a portion of the ingredients of Phase A with Phase B at 70-80°C, then cool in a spray-drying or "prilling" column to form beads. Then mix the beads in a portion of melted PEG 4000 and 8000, and add the drug, Polysorbate 80 and oleic acid to the mixture. Repeat this last step one to four times. EXAMPLE 2 Phase Ingredients %W/W B Cyclosporin 25 B Glyceryl Monooleate 15 A Peg-25 Glyceryl Trioleate 10 B Ceteryth-20 5 A Oleyl Alcohol 45 A Water q.s. Preparation: The emulεion core iε made by adding glyceryl monooleate, oleyl alcohol and ceteryth-20 in a heated vessel at > 40°C. Add the cyclosporin. Mix the PEG-25 glyceryl trioleate in water at 40°C. Prill a portion of the phase B materials, and suεpend them in a spray-drying apparatus. Alternate coats of phase A and phase B at least two times, and up to five times. EXAMPLE 3 Phase Ingredients %W/W A Calcitonin 30 B Vitamin E TPGS 10 C Medium Chain Mono and diglycerideslS D Polysorbate 80 5 E Hydroxypropyl ethyl cellulose 10 F Polyethylene Glycol 400 10 G Water 20 Preparation: To form emulsion core, first melt Vitamin E TPGS thoroughly, to > 40°C. Add a portion of the remaining ingredients to the melt. Heat water (G) to -70-80°C then add mix of ingredients A through F. While mixing using a εutiable mixer, the cores can be made in a spray-drying or "prilling" column. Alternate layers can be applied using the ingredients in a fluidized-bed apparatus or suspending the prills in a portion of the PEG 400 and mixing in the rest of ingredients and "re- prilling" the mixture. Repeat this latter procesε up to five timeε.

Claims: What Is Claimed Is : 1. A pharmaceutical preparation including a pharmaceutical agent incorporated into particles comprising (i) a core formed from a hydrophilic material, a hydrophobic material or a hydrophobic emulsion or dispersion and (ii) an alternating sequence of hydrophilic/hydrophobic layers thereon such that there is a hydrophilic/hydrophobic interface between the core and each succeeding layer. 2. The pharmaceutical composition of claim 1 wherein the particleε have a diameter of about 0.5 to 100 microns. 3. The pharmaceutical preparation of claim 2 wherein the particles have a diameter of lesε than about 10 microns. 4. The pharmaceutical preparation of claim 1 wherein the emulsion or disperεion iε εelected from the group consisting of a colloidal emulεion, microcolloidal emulsion and a microem lsion. 5. The pharmaceutical preparation of claim 4 wherein the long chain carboxylic acid is selected from • the group consisting of n-dodecanoic acid, n- tetradecanoic acid, n-hexadecanoic acid, caproic acid, caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, behenic acid, montanic acid, meliεεic acid, oleic acid, gadoleic acid, erucic acid, linoleic acid, linolenic acid, arachidonic acid, behenolic acid and diacetyl tartaric acid. 6. The pharmaceutical preparation of claim 1 wherein the long chain carboxylic acid eεter is selected from the group consiεting of glyceryl monostearates; glyceryl monopal itates; mixtures of glyceryl monostearate and glyceryl monopalmitate; glyceryl monolinoleate; glyceryl monooleate; mixtures of glyceryl monopalmitate, glyceryl monostearate glyceryl monooleate and glyceryl monolinoleate; glyceryl monolinolenate; glyceryl monogadoleate; mixtures of glyceryl monopalmitate, glyceryl monostearate, glyceryl monooleate, glyceryl monolinoleate, glyceryl monolinolenate and glyceryl monogadoleate; acetylated glycerides such as distilled acetylated monoglycerides; mixtures of propylene glycol monoesters, distilled monoglycerides, sodium stearoyl lactylate and silicon dioxide; mixtures of propylene glycol monoesters, distilled monoglycerides, sodium stearoyl lactylate and silicon dioxide; d-alpha tocopherol polyethylene glycol 1000 succinate; mixtures of mono- and di-glyceride esters; calcium stearoyl lactylate; ethoxylated mono- and di-glycerides; lactated mono- and di-glycerides; lactylate carboxylic acid ester of glycerol and propylene glycol; lactylic esters of long chain carboxylic acids; polyglycerol esters of long chain carboyxlic acids, propylene glycol mono- and di-esterε of long chain carboxylic acidε; εodiu εtearoyl lactylate; εorbitan monostearate; sorbitan monooleate; other sorbitan esters of long chain carboxylic acids; succinylated monoglycerides; stearyl monoglyceryl citrate; stearyl heptanoate; cetyl esters of waxes; cetearyl octanoate; C 10 -C 30 cholesterol/lavosterol esters; and sucrose long chain carboxylic acid esterε. 7. The pharmaceutical preparation of claim 1 wherein the long chain carboxylic acid alcohol iε selected from the group consisting of n-dodecanoic acid alcohol, n-tetradecanoic acid alcohol, n-hexadecanoic acid alcohol, caproic acid alcohol, caprylic acid alcohol, capric acid alcohol, lauric acid alcohol. myriεtic acid alcohol, palmitic acid alcohol, εtearic acid alcohol, arachidic acid alcohol, behenic acid alcohol, montanic acid alcohol, meliεsic acid alcohol, oleic acid alcohol, gadoleic acid alcohol, erucic acid alcohol, behenolic acid alcohol and diacetyl tartaric acid alcohol. 8. The pharmaceutical preparation of claim 1 wherein the hydrophobic emulsion comprises a self- emulsifying surface-active hydrophobic material weight/weight of the hydrophobic composition. 9. The pharmaceutical preparation of claim 8 wherein the self-emulεifying hydrophobic material iε selected from the groups of stearates, palmitates, ricinoleates, oleates, behenates, ricinolenates, myristates, laurates, caprylateε, and caproates. 10. The pharmaceutical composition of claim 1 wherein the 11. The pharmaceutical preparation of claim 1 wherein the surfactant is present in a range of about 0.05% to 2.0% weight/weight of the hydrophobic composition. 12. The pharmaceutical preparation of claim 1 wherein the surfactant is selected from poloxamers, sodium lauryl sulfate, sorbitan fatty acid esters and sucrose esters of fatty acidε. 13. The pharmaceutical preparation of claim 1 wherein the pharmaceutical agent has less than about 80% bioavailability. 14. The pharmaceutical preparation of claim 1 wherein the pharmaceutical agent is a polypeptide of up to about 15 amino acids. 15. The pharmaceutical preparation of claim 11 wherein the polypeptide of up to about 12 amino acids. 16. The pharmaceutical preparation of claim 1 wherein the pharmaceutical agent is an organic molecule of less than about 1,000 daltons. 17. The pharmaceutical preparation of claim 16 wherein the organic molecule is less than about 600 daltons. 18. The pharmaceutical preparation of claim 1 wherein the pharmaceutical agent is selected from cyclosporin, angiotensin I, II and III, enkephalins and their analogε, ACTH, antiinflammatory peptides I, II, III, bradykinin, calcitonin cholecystokinin fragments 26- 33 and 30-33, pre/pro cholecystokinin (V-9-M), β- endorphin, dinophin, leucokinin, leutinizing hormone releasing hormone, neurokinins, somatostatin, substance P, thyroid releasing hormone, vaεopressin, , acyclovir, adriamycin, cabamazepine, griseofulvin, angiotensin converting enzyme inhibitors, flutamide, melphalan, nifedipine, indomethacin, naproxen, estrogens, testosterones, steroids, phenytoin, sumatripan, ergotamines and cannabinoids. 19. The pharmaceutical preparation of claim 1 wherein the emulsion iε encapεulated in a capεule comprising an enteric coating material. 20. The pharmaceutical preparation of claim 1 wherein the microcolloidal emulsion is encapsulated in a capsule that is soluble in an acidic aqueous environment.

Inventors: BELENDUIK GEORGE W, MCCARTY JOHN A, RUDNIC EDWARD M, BELENDUIK, GEORGE, W., MCCARTY, JOHN, A., RUDNIC, EDWARD, M.
Applicants: PHARMAVENE INC, PHARMAVENE, INC.
Application year: 1995
Main classification: A61K9
IPC codes: A61K9