NOVEL 3-(SUBSTITUTED METHYL)-4-OXA-1-AZABICYCLO[3.2.0]HEPTAN-7-ONE DERIVATIVES USEFUL AS ANTITUMOR AGENTS

Abstract: The present invention is based on the discovery that certain 3-(substituted methyl)-4-oxa-1-azabicyclo[3.2.0]heptan-7-one derivatives of formula (I) or a pharmaceutically acceptable salt thereof are useful as antitumor agents against sensitive and resistant tumor cells. In Formula (I) X is NH or O, when X is NH, R is hydrogen, -COR1, wherein R1 is (i) a C1-C6 alkyl group which may be substituted by 1-3 substituents selected from halogen, hydroxy, formyloxy, azido, carboxyl, heteroaryl or acetamide, (ii) a C2-C4 alkenyl group, (iii) a C2-C4 alkynyl group, (iv) a C3-C6 cycloalkyl group, (v) a phenyl group which may be substituted by 1-3 substituents selected from hydroxy, halogen, C1-C6 alkoxy group or cyano, (vi) a C5-C6 heteroaryl group, (vii) an NR2R3 wherein R2 and R3 are the same or different and each is a hydrogen, C1-C6 alkyl group or (7-oxo-1-aza-4-oxabicyclo[3.2.0]hept-3-yl)methyl group or (viii) benzyloxy group; -SO2R4 wherein R4 is a C1-C6 alkyl group or a phenyl group which may be substituted by 1-3 substituents selected from C1-C6 alkyl group, halogen or C1-C6 alkoxy group; 1-2 amino acid residue which may be substituted with protective group; when X is O, R is 1-2 amino acid residue which may be substituted with protective group.

Description: NOVEL 3-(SUBSTITUTED METHYL)-4-OXA-l-AZABICYCLOr3.2.01 HEPTAN-7-ONE DERIVATIVES USEFUL AS ANTITUMOR AGENTS The present invention relates to novel 3-(substituted methyl ) -4-oxa-l-azabicyclo [ 3.2.0 ] heptan-7-one derivatives, and to the preparation of such compounds, and to their incorporation in pharmaceutical compositions, and to their use as pharmacological agents, in particular for use in anti-cancer treatment. Background of the Invention Since the invention of G0069A (JP 61-2125G7) and Tϋ 1718 (DE 3727651) , we paid attention to develop β-iactam class of compounds as antitumor agents. However, there were a lot of difficulties to obtain these compounds in large scales. For example, only 20 mg of G0069A was isolated from 10L fermentation broth even after under well controlled fermentation techniques and suitable experimental conditions. On the other hand, another β-lactam compound having the same stereochemistry at C5, (3R,5S)-3- hydroxymethyl-4-oxa-l-azai:icyclo [3.2.0]heptan-7-one (clava .1, G0069B) was isolated by Brown and Evans ( . Chem. Soc. Chem. Comm. 1979, 282) from the culture of streptomyces clavuligerus, which is reported to exhibit antifungal activity. We have synthesized clavam 2. and have done extensive biological evaluation. We found that clavam 1 exhibit excellent antitumor activity both in vitro and in vivo but is chemically unstable. G0069A TU1718 1 (G0069B) The chemical unstability may be due to the intermolecular interaction of -OH to the β-lactam ring which results in the decomposition of product. As a part of the program directed toward the development of β- lactam class of compounds as novel antitumor agents, it is necessary to get compounds which are relatively easy to synthesize, chemically stable and have strong antitumor activity. Summary of the Inventinn The present invention is based on the discovery that certain 3- (substituted methyl)-4-oxa-l-azabicyclo[3.2.0]he an-7-one derivatives exhibit excellent activity against sensitive and resistant tumor cells. In accordance to the present invention, there is provided a 3- (substituted methyl)-4-oxa-l-azabicyclo[3.2.0]hep an-7-one derivative of general formula I or pharmaceutically acceptable salt thereof, wherein X is NH or 0, when X is NH, R is hydrogen, -CORχ,wherein Ri is (i) a Ci-Cβ alkyl group which may be substituted by 1-3 substituents selected from halogen, hydroxy, formyloxy, azido, carboxyl, heteroaryl or acetamide, (ii) a C 2 -C 4 alkenyl group, (iii) a C 2 -C 4 alkynyl group, (iv) a C 3 -C 6 cycloalkyl group, (v) a phenyl group which may be substituted by 1-3 substituents selected from hydroxy, halogen, Ci-Cβ alkoxy group or cyano, (vi) a C 5 -C 6 heteroaryl group, (vii) a NR 2 R 3 wherein R 2 and R 3 are the same or different and each is a hydrogen, Cχ-C 6 alkyl group or (7-oxo-l-aza-4-oxabicyclo[3_2.0]hept-3- yl)methyl group or (viii) benzyloxy group; -SO 2 R 4 wherein is a Ci-Cβ alkyl group or a phenyl group which may be substituted by 1-3 substituents selected from Ci-Cβ alkyl group, halogen or Ci-Cβ alkoxy group; 1-2 amino acid residue which may be substituted with protective group, when X is 0, R is 1-2 amino acid residue which may be substituted with protective group. The pharmaceutically acceptable salts of formula I are selected from sodium, potassium, magnesium, calcium, hydrogen chloride, tartaric acid, succinic acid, fumaric acid or p-toluenesulfonic acid. Examples of Ci-Cg alkyl group as substitutents in R]_, R2 , R3 or R4 are straight or branched chain alkyl group having 1-6 carbon atoms such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec- butyl, tert-butyl, pentyl, 3-methylbutyl, hexyl and the like. Examples of halogen atoms as substituents in R-_ or R4 are fluorine, chlorine, bromine or iodine. Examples of heteroaryl group in R3 . are 1,2,3-triazole, 1,2,4- triazole, thiophene, pyridine and the like. Examples of C2-C4 alkenyl group as defined in R are alkenyl groups having 2-4 carbon atoms such as ethenyl, 1-propenyl, 2- propenyl, 1-butenyl, 3-butenyl and the like. Examples of C2-C4 alkynyl group as defined in Rη_ are ethynyl, 1- propynyl, 2-propynyl, 1-butynyl, 3-butynyl and the like. Examples of C3-C6 cycloalkyl group as defined in R are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and the like. Examples of heteroaryl group or heteroaryl substituent in Ri are C 5 -C 6 heteroaryl group which may have 1-3 heteroatoms selected from nitrogen and sulfar such as 1,2,3-triazole, 1,2,4 triazole, thiophene, pyridine and the like. - D - Example of Ci-Cβ alkoxy group as substituents in Ri or R are methoxy, ethoxy, propyloxy, butyioxy, pentyloxy, hexyloxy and the like. The term "amino acid residue" used herein refers to the remaining group after the removal of the hydroxy group from a carboxy group of an amino acid. The terr. "1-2 amino acid" used herein is one amino acid or one dipeptidε constituted of two amino acids which are bonded each other through a peptide bond. Example of amino acid are α-amino acids which compose organisms' protein or their optical isomers such as glycine, D- or L-alanine, D- or L-valine, D- or L-leucine, D- or L-isoleucine, D- or L- serine, D- or L-threonine, -D- or L-aspartic acid, D- or L-glutamic acid, D- or L-asparagine, D- or L-glutamine, D- or L-lysine, D- or L-arginine, D- or L-cysteine, D- or L-cystine, D- or L-mer.hionine, D- or L-phenylalanine, D- or L-tyrosine, D- or L-tryptophane, D- or L-histidine, D- or L-proiine and the like. Examples of protective group in amino acid residue are benzyl and/or acyl group such as acetyl, 2-thienylacetyl, benzyloxycarbonyl, tert-butcxycarbonyl and the like. Preferably, the derivatives of formula I wherein X is NH and R is -CORi wherein Ri is Ci-Cβ alkyl group which may be substituted by 1-3 substituents selected from halogen, hydroxy, forrπyloxy, azido, carboxy, heteroaryl or acetamido. Preferably, the derivatives of formula I wherein X is NH and R is -CORi wherein Ri is a NR 2 R3 wherein R 2 and R 3 are the same or different and each is a hydrogen, Ci-Cς, alkyl group or (7-oxo-l- aza-4-oxabicyclo[3.2.0]hept-3-yl)methyl group. Preferably, the derivatives of formula I wherein X is NH or 0 and R is 1-2 amino acid residue which may be substituted with protective group. Preferably, the derivative of formula I wherein X is NH, having (3R,5R) or (3S,5S) configuration at two asymmetric carbons on 4- oxa-1-azabicyclo [3.2.0]heptan-7-one ring system or the mixture cf them. Preferably, the derivative of formula I wherein X is 0, having (3R,5S) or (3S,5R) configuration at two asymmetric carbons on 4- oxa-1-azabicyclo[3.2.0]heptan-7-one ring system or the mixture of them. More specifically, when X is NH in general formula I, R is: hydrogen CORi wherein R-_ is selected from methyl, propyl, 1- methylethyl, chloromethyl, azido ethyl, (1,2,3- triazol-1-yl)methyl, formyloxymεthyl, hydroxymethyl, trifluoromethyl, pentyi, 5-bromopentyl, 5-formyloxy- pentyl, 5-azidopentyi, 5- (1,2, 3-triazol-l-yl)pentyl, 5- (acetamido)pentyi, cyclohexyl, 2-carboxyethyl, benzyloxy, phenyl, 4-hydroxyphenyl, 4-methoxyphenyl, 3,4-dimethyoxyphenyl, 4-fluorop enyl, 2, 5-difluoro- phenyl, 2,4,5-trifluorophenyl, 4-cyanophenyl, thiophen- 2-yl-methyl, pyridin-3-yl, diethylamino, (7-oxo-l- aza-4-oxabicyclo[3.2.0]hept-3-yl)methylamino, 3-butenyl, ethynyl, aminomethyl, N-acetylaminomethyl, N-(L-2-N- benzyloxycarbonylamino-3-methylbutyryl)aminomethyl, N- (L-2-amino-3-methylbutyryl) aminomethyl, N- (L-2-N- benzyloxycarbonylamino-3-benzyloxycarbonylpropanoyl) aminomethyl, N- (L-2-amino-3-carboxypropanoyl)amino¬ methyl, N- (D-4-N-benzyloxycarbonylamino-4-benzyloxy- carbonylbutyryl)aminomethyl, N- (D-4-amino-4-carboxy- butyryl)aminomethyl, L-1-N-benzyloxycarbonylaminoethyl, L-1-aminoethyl, D-1-N-benzyloxycarbonylaminoethyl, D-l- aminoethyl, L-l-N-benzyloxycarbonylamino-2-methyl- propyl, L-l-amino-2-methylpropyl, D-1-N-benzyloxy- carbonylamino-2-methylpropyl, D-l-amino-2-methyl- propyl) , L-l-N-benzyloxycarbonylamino-2-phnylethyl, L-l-amino-2-phenylethyl, L-1-N-benzyioxycarbonyl- amino-2-phenyiethyl, L-l-N- (L-2-N-benzyioxycarbonyi- amino-3-benzyloxycarbonylpropanoyl)amino-2-phenylethyl, L-1-N- ( -2-amino-3-carboxypropanoyl) amino-2-phenyl- ethyl, L-l-N-benzyloxycarbonylamino-2-benzyloxy- carbonylethyl, L-l-amino-2-carboxyethyl, D-3-N- benzyloxycarbonylamino-3-benzyioxycarbonylpropyi, D-l- amino-3-carboxypropyl, L-3-N-benzyloxycarbonylamino-2- hydroxyethyl or L-l-amino-2-hydroxyethyl . SO 2 R4 wherein R 4 is selected from methyl, 4-methyl- phenyl, 4-chlorophenyi or 4-methoxyphenyl group. When X is 0 in general formula I, R is; CORi wherein Ri is selected from aminomethyl, N- (N-benzyloxycarbonylaminoacetyi) aminomethyl, N- (aminoacetyl) aminomethyl, N- (L-2-N-benzyloxycarbonyl- aminopropanoyl) aminomethyl, N- ( -2-aminopropanoyl) - aminomethyl, N- (L-2-N-benzyloxycarbonylamino-3-methyl butyryl)aminomethyl, N- (L-2-N-bεnzyloxycarbonylamino-3- methylbutyryl)aminomethyl, N- (L-2-amino-3-methyl- butyryl)aminomethyl, L-l-N- (benzyloxycarbonyl) - a inoethyl, L-l-aminoethyl, L-l-N- (L-2-N-benzyloxy- carbonyamino-3-methylbutyryl)aminoethyi, L-l-N- (L-2-N- amino-3-methylbutyryl)aminoethyl, D-l-N- (benzyloxy¬ carbonyl)aminoethyl, D-1-aminoethyl, D-l-N- [D-2-N- (thiophen-2-yl) acetamidopropanoyl]aminoethyl, L-l-N- (benzyloxycarbonylamino-2-methylJpropyl, L-l-amino-2- methylpropyl, L-l-N- (2-N-benzyloxycarbonylaminoacetyl) - amino-2-methylpropyl, L-l-N- (2-aminoacetyl)amino-2- methylpropyl, L-l-N- (L-2-N-benzyloxycarbonylamino-3- methylbutyryl)amino-2-methylpropyl, L-l-N- (L-2-amino-3- methylbutyryl) amino-2-methylpropyl, L-l-N- (L-2-amino-3- carboxypropanoyl) mino-2-methylpropyl, L-l-N- (L-2-N- benzyloxycarbonylamino-3-benzyloxycarbonylpropanoyl) - amino-2-methylpropyl, D-l-N-benzyloxycarbonylamino-2- methylpropyl,D-l-amino-2-methyipropyi, D-l-acetamido-2- methylpropyl, D-l-N- (D-2-N-ben∑yioxycarbonylamino-3- methylbutyryl) ammo-2-methylpropyl, D-l-N- (D-2-amino-3- methylbutyryl) mino-2-methylpropyl, L-l-N- (benzyloxy- carbonyl) amino-2-phenyiethyl, L-i-amino-2-phenylethyl, L-2-N-benzyloxycarbonylamino-2-benzylcxycarbonylethyl, L-2-amino-2-carboxyethyl, L-1-benzyloxycarbonylamino- 2-benzyloxycarbonylethyl, L-l-amino-2-carboxyethyl, D- l-benzyloxycarbonylamino-3-benzyloxycarbonylpropyl, D- l-amino-3-carboxypropyl, L-l-ber.zyloxycarbonylamino-2- benzyloxyethyi, L-l-N- (D-2-N-benzyloxycarbonylamino- propanoyl)amino-2-hydroxyethyl, L-1-N-benzyloxycarbonyl- amino-2-hydroxyethyl, L-l-N- (D-2-N-benzyloxycarbonyl- aminopropanoyl) amino-2-benzyloxyethyl, L-l-N- (L-2-N- benzyloxycarbonylamino-3-methylbutyryl) amino-2- benzyloxyethyl, L-l-N- (L-2-amino-3-methylbutyroyl) - amino-2-benzyloxyethyl, L-i-acetamido-2-acetyloxyethyl, L-l-acetamido-2-hydroxyethyl, L- [1, 5-di- (benzyloxy- carbonylamino)pentyi] , L- [1, 5-di- (acetamido)pentyi] or L-5-acetamido-l-N-ben∑yloxycarbonylaminopentyl. Examples of pharmaceutically acceptable salts are sodium, potassium, calcium, magnesium or hydrogen chloride, tartaric acid, succinic acid, fumaric acid or p-toluenesulfonic acid. Compounds of formula I may be utilized as antitumor active compounds in medicaments, being formulated with a pharmaceutically acceptable carrier. The bicyclic nucleus carries two asymmetric carbon atoms at position 3 and 5 and can exist as 4-diastereoisomers. In general, the preferred isomer is that in which the hydrogen atoms at C3 and C5 are trans to each other for superior toxicity against different malignant cells such as P388, KE, NUGC4, WI38, L-1210, sarcoma 180 and colon 26. Such diaεterioisomers and their race ic mixtures are also included within the use of the oxapenam derivatives as antitumor agents. Antitumor activity of compounds described above is expected - q - against some solid cancers such as stomac lung, breast, liver, uterus and leukemia and sc on. Description of Preferred Embodiment The present invention relates to the certain 3- (substituted methyl) -4-oxa-l-azabicyclc[3.2.0]heptan-7-one derivatives having excellent antitumor activity. The compounds of this invention are characterized by having ester (-OCR]_) , amide (NHCORχ) and sulfonamide (NHSO2R4) linkage with alkyl, aryl, heteroaryl, amino acids or dipeptide groups which were prepared by the common starting compound 2- The preparation of compound 2. was carried out by the synthetic route as described ir. J. Chem. Soc. Perkin Trans. I 2222, 1980, starting from 4-acetcxyazetidinone. Wherein L and Y are suitably selected from leaving groups such as chlorine, bromine, iodine, methanesulfonyloxy group, trifluoro- methanesulfonyloxy group, p-toluenesulfonyloxy group and p- chlorobenzenesulfonyloxy group. The preparation of substituted aminomethyi-4-oxa-l-azabicyclo- [3.2.0]heptan-7-one of general formula I was done by synthetic route as shown below. Wherein M is sodium, potassium or trimethvlsilyl and Rι_ is- a Cχ-C 6 alkyl group which may be substituted by 1-3 substituents selected from halogen, hydroxy, formyloxy, azidc, carboxyl, heteroaryl or acetamide; a C 2 -C 4 alkenyl group; a C 2 -C 4 alkynyl group; a C 3 -C 6 cycloalkyl group; a phenyl group which may be substituted by 1-3 substituents selected from hydroxy, halogen, Cχ-C 6 alkoxy group or cyano; a C 5 -C 6 heteroaryl group; a NR 2 R 2 wherein R2 and R 3 are the same or different and each is a hydrogen, Cχ-C 6 alkyl group or (7- oxo-l-aza-4-oxabicyclo[3.2.0]hept-3-yl)methyl group or bezyloxy group; . Certain 3-substituted amidomethyl-4-oxa-l-azabicyclo[3.2.0] - heptan-7-one general formula I when substituent of R-_ in -COR ] _ is acetamide, azide, formyloxy, hydroxy and heteroaryl, were prepared by the scheme as shown below. NHCO(CH 2 )r . NHCOCH 3 1 2 1 1 1 4 1 3 Wherein n is 1 to 5; M is defined as above. Besides the procedure described above the stereoisomer of 3- acetyaminomethyl-4-oxa-i-azabicyclo[3.2.0]heptan-7-one(8,R=CH 3 ) were prepared by the scheme as described below starting from 3- hydroxymethyl-4-oxa-l-azabicyclo [3.2.0]heptan-7-one(1) . 1 5 ΓT JHCOCH 3 Certain derivatives of formula I wherein X is NH and R is SO 2 R , were prepared by following the scheme as shown below starting from 3-azidomethyl-4 -oxa-l-azabicyclo [ 3 . 2 . 0 ] heptan-7 -one ( 6 ) . 16 Wherein R4 is the same as def ined above . The amide derivative of amino acids and dipeptides of general formula I were alternatively prepared by the synthetic scheme as shown below in two steps. In the first step, 3-aminomethyl-4-oxa- l-azabicyclo[3.2.0]heptan-7-one(7) was coupled with either N- protected acid in the presence of dicyclohexylcarbodiimide (DCC) or acid chloride in the presence of a base or activated ester followed by coupling with another N-protected amino acid which may or may not be the same. The resulting compounds were deprotected in the presence of H2 and Palladium catalyst under pressure or atmospheric pressure. > NHCOCH(R 5 )NHZ 1 7 NHCOCH(R 5 )NHCOCH(R 6 )NHZ NHCOCH(R 5 )NH 2 1 9 1 8 NHCOCH(R 5 )NHCOCH(R 6 )NH 2 2 0 Wherein R5 and Rg are selected from hydrogen, methyl, 1-methyl- ethyl, phenylmethyl, 2-carboxyethyl, carboxymethyl, hydroxymethyl, benzyloxymethyl, acetyloxymethyl, aminobutyl and Z is benzyloxy- carbonyl, acetyl, 2-thienylacetyl or tert-butoxycarbonyl. The ester derivative of amino acids and dipeptides were prepared by the coupling of 3-bromomethyi-4-oxa-l-azabicyclo[3.2.0]heptan- 7-one(2) with N-protected aminoacids in the presence of a base. The amine group was deprotected and couples with another molecule of amino acid as shown in the scheme below. 2 1 22 23 24 Wherein R5, Rg and Z are defined as above. In the above descriptions, the reactants are reacted together with solvent at elevated or low temperatures for sufficient time to allow the reaction to proceed to completion. The reaction conditions will depend upon the nature and reactivity of the reactants. Wherever a base is used in a reaction, they are selected from triethylamine, pyridine, 4-dimethylamino-pyridine, diisopropylethyla ine, 1, 5, -Dizabicyclo[4.3.0]non-5-ene, 1,8- Dizabicyclo[5.4.0]undec-7-ene, sodium carbonate, potassium carbonate or cesium carbonate. The solvents of choice for the reaction are non reactive solvents such as acetonitrile, tetrahydrofuran, ethanol, methanol, chloroform, ethylacetate, methylene chloride, dimethylformamide, dimethylsulfoxide, hexamethylphosphoric triamide or the like. Solvent mixtures may also be utilized. Reaction temperatures would generally range from between -70°C to 140°C. The preferred molar ratio of reactants are 1:1 to 5.0. The reaction time range from C.5 to 72 hours, depending on the reactants . The deprotection of N-prctective group is carried out either by hydrogenation in the presence of acids, such as hydrochloric acid or acetic acid, or by hydrolysis with appropriate acids, such as hydrochloric acid, triflucriacetic acid cr acetic acid in solvents such as methanol, ethanol, propanol or ethylacetate. The hydrogenation reaction is usually carried out in the presence of a metal catalyst, such as Pά, Pt or Rh, under normal pressure to high pressure. The compound of the invention, when used as an agent for treating malignant tumors of mammals including humans, may take pharmaceutical dosage forms including parenteral preparations such as injections, suppositories, aerosols and the like, and oral preparations such as tablets, coated tablets, powders, granules, capsules, liquids and the like. Injections are generally preferred. The above preparations are formulated in a manner known in the art. For the formulation of solid preparations for oral administration, an excipient, and if desired, a binder, disintegrator, lubricant, coloring agent, corrigent, flavor, etc. are added to the compound of the invention, and then tablets, coated tablets, granules, powders, capsules or the like are prepared in a conventional manner. For the formulation of injections, a pH adjusting agent, buffer, stabilizer, isotonic agents, local anesthetic or the like is added to the active ingredient of the invention, and injections for subcutaneous, intramuscular or intravenous administration can be prepared in a conventional manner. For the formulation of suppositories, a base, and if desired, a surfactant are added to the active ingredient cf the invention, and the suppositories are prepared in a conventional manner. The excipients useful for the sclid preparations for oral administration are those generally used in the art, and useful examples are excipients such as lactose, sucrose, sodium chloride, starches, calcium carbonate, kaolin, crystalline cellulose, methyl cellulose, glycerin, sodium alginate, gum arabic and the like, binders such as polyvinyl alcohol, polyvinyl ether, polyvinyl pyrrolidone, ethyl cellulose, gum arabic, schellac, sucrose, water, ethanol, propanol, carboxymethylceilulose, potassium phosphate and the like, lubricants such as magnesium stearate, talc and the like, and further include additives such as usual known coloring agents, disintegrators and the like. Examples cf bases useful for the formulation of suppositories are, for example, oleaginous bases such as cacao butter, polyethylene glycol, lanolin, fatty acid triglycerides, Witepsol (trademark, Dynamite Nobel Co., Ltd.) and the like. Liquid preparations may be in the form of aqueous cr oleaginous suspension, solution, syrup, elixir and the like, which can be prepared by a conventional way using additives . The amount of the compound (I) of the invention to be incorporated into the pharmaceutical composition of the invention varies with the dosage form, solubility and chemical properties of the compound, administration route, administration scheme and the like. Preferably the amount is about 1 to 25 w/w% in the case of oral preparations, and about 0.1 to about 5 w/w% in the case of injections which are parenteral preparations. The dosage of the compound (I) of the invention is suitably determined depending on the individual cases taking symptoms, age and sex of the subject and the like into consideration. Usually, the dosage in the case of oral administration is about 50 to 1000 mg per day for an adult in 2 to 4 divided doses, and the dosage in the case of injection, fcr example, by intravenous administration is 2 ml (about 1 to 50 mg) which is administered once a day for adults wherein the injection may be diluted with physiological saline or glucose injection liquid if so desired, and slowly administered over at least 5 minutes. The dosage in the case of suppositories is about 1 to 500 mg which s administered once or twice a day at an interval of 6 to 12 hours wherein the suppositories are administered by insertion into the rectum. Reference Example 1 (3R.5S)-3-f (4-Chiorobenzenesulfpnyl)oxyms hyl ■ -4-oxa-l-azabicvclo r..2.0.hgnr.an-7-one A mixture of (3R, 5S) -3-Hydroxymethyl-4-oxa-l-azabicyclo [3.2.0] heptan-7-one (790mg) , Triethylamne (726mg) , 4-Chlorobenzene- sufonyl chloride (1.51g) m Dichloromethane was stirreed for lβhrε. The reaction mixture was purified by silicagel column chromatography, using Chloroform-Ethyl acetate (5:1) as eluent and desired sulfonate was obtained as solid. Yield: 84% m.p. :153.6-155.1°C, [α] : D s =-90.0° (c=0.67, CDC1 3 ) !H NMR(CDCl3,δ) : 2.82 (IH, d,J=16.1) , 2.8 (IK, dd,J=5.6 , 5.9) ,3.28 (IK, ddd,J=1.0,2.9,16.1) ,3.98 (IH, dd, =7.1, 11.7 ) ,4.09 (IH, dd,J= .4 , 10.8) ,4.21 (lH,dd,J=3.6,10.8) , .47-4.58 (lH,m) , 5.27 (IH, d,J=2.6) , 7.56(2H,d,J=8.7) , 7.86 (2H, d,J=8.7 ) . IR(Nujol,cm _1 ) :1776,1450. Reference Example 2 ns.5S)-3-Azidomethvl-4-oxa-l-azabicvclc r ~ .2. Ql entan-7-one The sulfonate of reference example 1 (1.45g) was treated with Sodium azide (767mg) in Dimethylformamide (10ml) at 70°C for 2.5hrs. The reaction mixture was diluted with Ethyl acetate, washed with water, brine and dried over Magnesium sulfate. The solvent was removed in vacuo and the residue was purified by silicagel column chromatography using Hexane-Ethyl acetate (2:1) as eluent. Yield:94%(oil) [«]"=-159.0° (c=0.67,CDCl 3 ) 3-H NMR(CDCl3,δ) :2.84 (IH, dd,J=ll .7 , 6.9) , 2.67 (IH, d,J=16.2) ,3.26- 3.38 (2H,m) ,3.54(lH,dd,J=3.7,13.2) , 3.98 (IK,dd,J=6.7 , 11, 7) ,4.43- 4.54 (lH,m) ,5.40(lH,d,J=2.6) . IR(Neat,cm _1 ) : 2960 , 2100, 1778. - IP - Reference Example 3 Aliyl r (3R5.5RS) -7-o χ p-l-aza-4-oxabicvclc " 3.2.0 ' ept-3-yll methylamino succinate 300mg of (3RS, 5RS) -3-Azidomethyl-4-oxa-l-azabicyclo [3.2.0]heptan -7-one was hydrogenated with 200 mg of 5% Palladium on activated carbon in 30ml of Ethyl acetate at 50 psi for Ihr . After removal of catalyst by filtration, 20mg ( 0.21mmol) cf Triethylamine, 360mg (2.30mmol) of Allyl hydrogen succinate and 480mg (2.30mmol) of 1,3 -Dicyclohexyl carbodiimiάe were added under ice cooling. The reaction mixture was stirred at room temperature for 4hrε. The resulted solid was filtered off and the filtrate was concentrated. The residue was purified by silica gel column using Hexane- Ethylacetate (1:1) as eluent. 150mg of title compound was obtained. Yield:30% J-H NMR(CDCl3,δ) : 2.47-2.96 (6H,m) , 3.25-3.59 (3H,m) , 3.94 (IH, dd,-J=6.4 , 11.8) ,4.30-4.42 (lH,m) , .57-4.61 (2H,m) , 5.21-5.37 (3H,m) ,5.81-6.04 (2H,m) . IR(Neat, cm "1 ) :3330,2955, 1783 , 1736. Example ι (3RS.5RS) -3- (N-Acetvlaminomethvi) -4-oxa-l-azabicvclo [ .2.01 heptan-7-one (1) 2.75g of (3RS, 5RS) -3-Azidomethyl-4-oxa-l-azabicyclo [3.2.0]heptan- 7-one, prepared by the known method, was hydrogenated over l.Og of 10% Palladium on activated carbon in Ethyl acetate at 50psi for lhr. After removal of catalyst by filteration, 2.3ml of Triethylamine was added and then 1.2ml cf Ace yl chloride was added dropwise at 5°C. The reaction mixture was then stirred for 2hrs. The resulting mixture was loaded on flash silicagel column and eluted with Ethyl acetate. The title compound was obtained as white solid. Yield: 67% m.p. :59-61°C X H NMR(CDCl3,δ) :2.02 (3H, s) , 2.6 (IH, d,J=c .5 , 11.7) , 2.8 (J=lβ.2) , 3.26-3.39 (2H,m) , 3.56 (lH,ddd,J=3.6, 6.5, 1 . Ξ ) , 3.96 (lH,dd,J=6.5, 11.9 ),4.37 (lH,m) , 5.32 (IH, ,J=2 , 7) , 5.80 (lH,br. s) . IR(Nujol,cm _1 ) : 3565, 1768, 1646, 1540. Example 2 (3S.5S) -3- (N-Acetylaminomethyl) -4-oxa-l-azabicvclo[3.2. Olheotan- 7-one (2) (3S,5S) -3-Azidomethyl-4-oxa-l-azabicyclo[3.2.0]heptan-7-one of reference example 2 (710mg) was hydrogenated in presence of Acetic anhydride (431mg) Ethyl acetate (20mi) at 50psi for lhr. The reaction mixture was purified by silicagel column chromatography using Ethyl acetate-Acetone (5:1) as eluent and the title compound was obtained as solid. Yield: 89% m.p. :82.5-84.5°C, CHC1 3 ) ! H NMR(CDCl3,δ) :2.02(3K,s) , 2.68 (IH, dd, J=7. , 11.8) ,2.84(1H, d, J=16.2) ,3.26-3.40(2H,m) , 3.56 (IH, ddd, J=3.6 , 6.3 , 14.2) ,3.96(lH,dd, J=6.4, 11.8) ,4.31-4.43 (lH,m) , 5.33 (IH, d, J=2.5) , 5.86 (lH,br . s) . IRWujol.cπT 1 ) : 3340, 1764, 1646, 1540. Example 3 (3R.5R) -3- (N-Acetylaminomethyl) -4-oxa-l-azabicvclo [3.2. Olheotan- 7-one (3) According to the similar method as described m reference example 1,2 and example 2, the title compound was obtained from (3S,5R)- 3-Hydroxymethyl-4-oxa-l-azabicyclo[3.2.0]heptan-7-one. Yield:87% m.p. :86.0-87.6°C , [θ.] : D '=+192.0° (c=1.0, CHC1 3 ) 1 H NMR(CDCl3,δ) :2.02 (3K,s) , 2.68 ( IH, dd, _T=7.0, 11. S ) , 2.84 (IK, d, J=16.5) ,3.26-3.40(2H,m) , .56 (IK, dd, J=3.6 , 6.3 , .2) ,3.96(lH,dd, J=6.4, 11.8) ,4.31-4.43 (lH,m) , 5.33 (IH, , =2.6) , Ξ .87 (lH,br .s) . IRWujo^cπr 1 ) : 3345, 1764, 1646, 1542. Example 4 (3RS.5R£)-3-(N-Hexanovlaminome hyl)-4-oy=.-i-azabicvclo.3.2.01 heptan-7-one (4) According to the same method as desribed in example 1, the title compound was obtained as solid. Yield:41% m.p. :56-57°C *H NMR(CDCl3,δ) : 0.90 (3H, t,J=6.6) , 1.27-1.40 (4H,m) , 1.56-1.7l ' (2H,m) , 2.20(2H,t,J=7.9) ,2.68 (IH,dd, =7. C, 11.8) , 2.84 (IH,d,J=16.1) ,3.26- 3.42(2H,m) ,3.55(lH,ddd,J=3.8,6.3,14.3) , 3.95 (IH,dd,J=6.5, 11.8) , 4.31-4.43 (lH,m) , 5.32 (IH,d,J=2.6) , 5.87 (lK,br. ε) . πU ujol, - 1 ) :3320, 1762, 1659, 1531. Example 5 (3RS.5RS) -3- TN- (2-Methvlpropanovl) aminomethvll -4-oxa-l-azabicvclo ■3.2.0.heptan-7-one (5) According to the same method as desribed in example 1, the title compound was obtained as solid. Yield:53% m.p. :76-77°C X H NMR(CDCl3,δ) : 1.17 (6H, ,J=6.9) , 2.35-2. 2 (IH, ) , 2.67 (IH,dd,J= 7.0,11.7) ,2.85(lH,d,J=16.3) , 3.27-3.42 (2K,m) , 3.51-3.65 (lH,m) , 3.96 (lH,dd,J=6.4,11.8) ,4.30-4.45 (IK, ) ,5.32 (IK, s) , 5.75 (lH,br.ε) . IR(Nujol,cm -1 ) :3325, 2960, 766. Anal . : calcd: C, 56.59; H, 7.60; N, 13.2C Found: C, 56.36; K, 7.79; X, 12.9S Example 6 (3RS.5RS) -3- (N-Cvclohexyloarbonylaminometr.yl) -4-oxa-l-azabicvclo r3.2.01heptan-7-one (6) According to the same method as desribed in example 1, the title compound waε obtained as solid. m.p. :83-85°C Yield: 5% -K NMR(CDCl3,δ) : 1.14-1.95 (10H,m) , 2.02-2.2C (IH,m) , 2.60-2.72 (IH, dd, J=6.8,11.7) ,2.84(lH,d,J=16.2) , 3.22-3.42 (2K,m) , 3.46-3.64 (lH,m) , 3.95 (lH,dd,J=6.5,11.8) , 4.31-4.43 (lK,m) ,5.33 (IH, d,J=2.7) 5.68-5.82 (lH,m) . IRlNujol,™- 1 ) :3305, 2930, 1781. Exa ple 7 (3RS.5RS) -3- ΓN- (Trifluoroacetvl) aminomethvll -4-oxa-l-azabicvclo r3.2.01hentan-7-one(7) According to the same method as desribed in example 1, the title compound was obtained as solid. Yield:74% m.p. :54-57°C lH NMR(CDCl3,δ) : 2.78 (IH, dd,J=6.7 , 11.5) , 2.89 (IH, d,J=16.8) ,3.30- 3.49 2H,m) , 3.68 (IH, ddd,J=2.4, 6.5 , 14.3 ) , 4.03 (IH, dd,J=6.5 , 11.9) , 4.37-4.49 (lH,m) , 5.36 (IH,d,J=2.7) , 6.62 (lH,br. ε) . IRWujol.cirT 1 ) : 3300, 1773, 1710, 1550. Example P (3RS.5RS)-3-(N-Chloroacetviam nomethvl)-"-oxa-l- azabicvclo .3.2.0.heptan-7-one (8) According to the same method as desribed example 1, the title compound was obtained as solid. Yield:51% m.p. :65-66.4°C ! H NMR(CDCl3,δ) :2.68(IH,dd,J=6.6,11.7) ,2.67 (IH,d,J=16.0) ,3.32(1H, dd,J=2.2,16.7) ,3.43-3.50(IK,m) ,3.60(IH,ddd,J=3.6, 6.2,14.2) ,3.99 (lH,dd,J=6.5,11.9) ,4.09 (2K,ε) ,4.36-4.46 (IK,m) ,5.36(IH,d,J=2.6) , 6.88(lH,br.ε) . IR(Nujol,cm _1 ) =3315,1764. Example 9 (3RS.5RS) -3-ΓN- (Azidoacetvl)aminomethvll-4-oxa-l-azabicvclo ■3.2.01heptan-7-one (9) (1) (3RS, 5RS)-3-(N-Chloroacetylaminomethyi) -4-oxa-l-azabicyclo [3.2.0]heptan-7-one(500mg) , of example 8, and Sodium azide(297mg) were stirred in Dimethyl sulfoxide(lθ l) at room temperature for 14hrs. The reaction mixture waε diluted with Ethyl acetate, washed with water, brine and dried over Magneεium εulfate. The residue, which obtained after removal of solvent in vacuo, was purified by εilicagel column chromatography (Hexane + Ethyl acetate 1:2). The title compound was obtaine as oil. Yield:89% (2) (3RS,5RS) -3-Azidomethyl-4-oxa-l-azabicyclo[3.2.0]heptan-7-one (l.Og) was hydrogenated with 5% Palladium on activated carbon (250mg) in Ethyl acetate(20ml)at 50psi for 2hrε. To the reaction mixture, Triethylamine(12l g) , Azidoacetic acid(1.21g), 1,3- Dicyclohexyl carbodiimide(2.46g) and Ethyl acetate(10ml) was added and stirred for lhr at room temperature. After removal of εolid by filtration, the solvent was removed in vacuo and the residue was purified by silicagel column chromatography (Hexane- Ethyl acetate 1:3) . Desired azidcacεtamide was obtained as oil. Yield:52%. (3) A mixture of (3RS, 5SR) -3-Bromomethyl-4-oxa-i-azabicyclo [3.2.0]heptan-7-one (l.Og) , Azidoacetic acid (735mg) , Triethylamine (737mg) and Hexamethylphosphoric triamide (5ml) was heated with stirring at 60-70°C fcr 24hrs. The reaction mixture was then diluted with Ethyl acetate, washed with water, brine and dried over Magneεium sulfate. The residual oil after removal of solvent in vacuo, was purified by silicagel column chromatography using Hexane-Ethyl acetate (2:3) as eluent. The desired azidoacetate was obtained aε oil. Yield:36%. ~H NMR(CDCl3,δ) :2.81 (IH,dd,J=6.2 , 1 .7) , 2.67 (IH, d,J=16.2) ,3.28 (lH,ddd,J=0.7,2.8,16.2) , 3.95 (2K, s) , 4.02 (IK,dd,J=6.9 , 11, 7) , 4.29 (2H,d,J=7.8) ,4.53-4.65 (lH,m) , 5.35 (IH,d,_J=2.7) . IRtNeat^m "1 ) .2115,1784,1751,1193. Example 10 (3RS.5RS) -3- rN- (1.2. -Triazole-1-vl)acetviaminomethvll -4-oxa-l- azabicvclor3.2.01hentan-7-one (10) 150mg of (3RS, 5RS) -3- [N- (Azidoacetyl) minomethyl] -4-oxa-l- azabicyclo[3.2.0]heptan-7-one was dissolved in 10ml of Acetone and Acetylene was bubbled at -78°C for 5 minutes. The reaction mixture was heated at 60-70°C in seal tube for 17hrs. After reaction completed, solvent was removed in vacuo and the residue was purified by silicagei column chromatography (Ethyl acetate + Acetone 3:1) . The title compound was obtained as oil. Yield:69% H NMR(CDCl3,δ) :2.57 (IH,dd,J=6.9 , 11.7) ,2.82 (IH, d,J=16.0) ,3.28 (lH,dd,J=2.9,16.8) ,3.40-3.51 (2H,m) , 3.94 (IH,dd,J=6.5, 11.9) , 4.29- 4.41 (lH,m) ,5.12 (2H,ε) ,5.24 (lH,d,J=2.6) ,7.73 (lH,d,J=0.8) ,7,80 ( lH , d , J=0 . 7 ) . IR ( Neat , cirr 1 ) : 3310 . 1779 , 1689 , 1555 . Example 11 (3RS.5RS) -3- [N- (Formyloxyacetyl) aminomethyll -4-oxa-l-azabicvclo r3.2.Q]hentan-7-one (11) A mixture of (3RS, 5RS) -3- (N-Chloroacetylaminomethyl) -4-oxa-l- azabicyclo[3.2.0]heptan-7-one(260mg) , Sodium formate(97mg) in Dimethyl sulfoxide(5ml) was stirred at room temperature for 15hrs, then 70°C for 3days. The reaction mixture was poured into water, extracted with Chloroform and dried over Magnesium sulfate. The residue which obtained after removal of solvent in vacuo, was purified by flash chrmatography (Ethyl acetate) . The title compound was obtained as oil. Yield:33% H NMR(CDCl3,d) :2.68 (IH,dd,J=6.7 , 1.6) , 2.86 (IH,d,J=15.9) , 3.27- 3.47 (2H,m) ,3.64(lH,ddd,J=3.5,6.4,14.2) , .34-4.46 (lH,m) ,4.69 (2H,s) ,5.34(lH,d,J=2.6) , 6.48 (IH,br.ε) , 8.16 (IH,ε) . IR(Neat,cm "1 ) :3315, 1770, 1719, 1667 , 1537. Example 12 (3RS.5RS) -3- rN-(Hvdroxvacetvl)aminomethvll -4-oxa-l -azabicvclo r3.2.01hentan-7-one(12) To a solution of (3RS, 5RS) -3- [N- (Formyloxyacetyl) aminomethyl] -4- oxa-l-azabicyclo[3.2.0]heptan-7-one(55mg) in a mixture of Methanol(lml) and water (0.1ml), Sodium hydrogenecatbonate(2Omg) was added and stirred at room temperature for 10 minutes. Reaction mixture waε loaded directly on flash column and eluted with Ethyl acetate + Acetone (1:2) . The title compound was obtained as solid. Yield:69% m.p. :103-105°C 1 H NMR(CDCl3,δ) :2.54 (IK, , =5.1) , 2.70 (IH, dd,J=7. , 12.2) , 3.31 {IH, dd, =2.2, 15.9) , 3.44 (IH,d ,J=7. E , 14.2) , 3.60 (IH, ddd,J=3.8 , 5.3 , 14.2) ,3.98(lH,dd,J= 6.5,11.8) ,4.16 (2H,d,J=5.1) ,4.35-4.47 (lH,m) ,5.35 (lH,d,J=2.6) , 6.80 (lH,br.s) . IR(Nujol,cm- 1 ) : 3330,1769, 1650, 1531. Example 13 (3RS.5RS) -3-rN-(6-Bromohexanovl) aminomethyll -4-oxa-l-azabicvclo [3.2.01heptan-7-one (13) According to the same method as deεribed m example 1, the title compound was obtained as oil. Yield:50% *H NMR(CDCl3,δ) :1.50-2.40 (8H,m) ,2.67 (IH,dd,J=6.9, 11.7) ,2.85 (lH,d,J=16.1) , 3.38-3.76(5K,m) ,2.96 (IH,dd,J=6.4, 11.8) ,4.43-4.56 (lH,m) ,5.33(lH,d,J=2.6) ,5.80-5.93 (lH,m) . IR(Neat, cirr 1 ) :3310, 1772, 1639. Example 14 (3RS.5RS) -3- fN- ( -Formvloxvhexanovl)aminomethvll -4-oxa-l- azabjcyclgr3■2.Q1he t&n-7-one (14) According to the same method as desribed in example 11, the title compound waε obtained aε εolid. Yield:25%. m.p. :61.5-63°C -K NMR(CDCl3,δ) :1.37-1.81 (6H,m) , 2.22 (2H, ,J=7.5) ,2.67 (lH,dd, J=7.0,11.7) ,2.85(lH,d,J=16.2) , 3.28-3.65 (3K,m) ,3.96(lH,dd, J=6.4,11.8) ,4.17(2H,t,J=6.6) , .32-4.42 (IK, ) ,5.32 (IH,d,J=2.6) , 5.74(1H br.ε) ,8.06(lH,ε) . IR(Nujol,cm _1 ) :3315, 1763, 715 Example 15 (3RS.5RS) -3-[N- (6-Azidohexanoyl)aminomethyll -4-oxa-l-azabicvclo [3.2.01heptan-7-one (15) According to the same method aε desribed in example 9, the title compound waε obtained aε oil. Yield:70%. X H NMR(CDCl3,δ) :1.33-2.30 (7H,m) ,2.62-2.72 (IH,dd,J=6.7,11.7) ,2.84 (lH,d,J=16.0) ,3.22-3.65(5H,m) ,5.92(IH,dd,J=6.5.11.8) ,4.30-4.45 (lH,m) ,5.32(lH,d,J=2.6) ,5.73-5.85(lH,m) . IR(Neat,cm -1 ) :3310,2935, 1772,162 . Example 16 (3RS.5RS)-3-ΓN- [(1.2.3-Triazol-l-yl)hexancvilaminomethyll-4-oxa-l- azabicvclor3.2.01heptan-7-one ( 6) According to the same method as desribed in example 10, the title compound was obtained as solid. Yield:37% m.p. :108-109°C X H NMR(CDCl3,δ) :1.24-2.04 (6H,m) ,2.21(2H,t, =7.3) ,2.65 (IH,dd,J=7.2, 11.9),2.83(lH,d,J=16.3) ,3.24-3.61(3H,m) ,3.94 (IH,dd,J=6.4,11.8) , 4.30-4.46 (3H,m) ,5.31(lH,d,J=2.6) ,5.74-5.67 (lH,m) ,7.55(lH,ε) ,7.71 (lH,ε) . IR(Nujol,cm _1 ) :3315,2945,1783,1715. - 23 - Example 17 (3RS.5RS) -3- .N- (6-Acetylaminohexanoyl) aminomethyl " -4-oxa-l- azabicvclo. .2. Olheptan-7-one (17) According to the same method aε deεcribed in example 10, the title compound was obtained as solid. Yield:10% m.p. :117-118°C X H NMR(CDCl3,δ) :1.33-1.76 (oH,m) , 1.97 (3H, s) , 2.21 (2H, t,J=7.2) ,2.68 (lH,dd J=7.0,11.9) , 2.86 (IK,d,J=16.3) , .21-3.66 (5K,m) ,3.98(lH,dd, J=6.4,11.8) ,4.30-4.43 (lH,m) , 5.32 (IH,d,J=2.7) , 5.52-5.65 (lH,m) , 5.78-5.92(lH,m) . IR(Nujol,cm _1 ) :3275, 1776, 634. Example 18 Sodium (N-r (3R. q .5RS)-7-oxc-l-aza-4-oxabicvclof3.2.0]hept-3-yll methylamino)succinate (18) The protected compound obtained in reference example 3 was dissolved in a mixture of Ethyl acetate and Dichloromethane (1:1, 4ml) and Sodium 2-ethylhexanoate(0.30ml) , Triphenylphosphine (13mg) , and Tetrakis (triphenylphosphine)palladium(0) (22mg) were added. The reaction mixture waε εtirred at room temperature for 2hrs and solid was collected by suction. Solid was washed with Acetonitrile and dried. 65mg of the title compound was obtained as white solid. Yield:46% m.p. :100°C(dec. ) . IH NMR(DMSO-d 6 ,δ) :2.08-2.25 (4H,m) , 2.61-2.81 (2H, ) ,3.11-3.36 (3K, m) , 3.76(lH,dd,J=6.6,11.5) ,4.27-4.33 (lH,m) , 5.33 (IH,d,J=2.5) ,8.86-8.98 (lH,m) . IRWujol,™- 1 ) .3330,1781,1651 Example 19 (3R5.5RS) -3- [N- ( -Pentenoyl)aminomethyl < -4-oxa-l-azabicvclo[3.2.0 1 heotan-7-one (19) 60Omg of (3RS, 5RS) -3-Azidomethyl-4-oxa-l-azabicyclo[3.2.0]heptan- 7-one was hydrogenated with l.Og of 10% Palladium on activated carbon in Ethyl acetate at 50pεi for lhr. After removal of catalyst by filtration, 360mg (3.6mmol) cf 4-Pentenoic acid and 300mg (l.δmmol) of 1, 3-Dicyclohexyl carbcdiimide were added under ice-cooling. The reaction mixture was then stirred at room temperature for 2hrs. Reεulted solid was filtered off and the filtrate waε concentrated in vacuo. The residue was purified by flash silicagel column using Hexane-Ethyl acetate (1:1) as eluent and the title compound waε obtained aε oil. Yield:15%. X H NMR(CDCl3,δ) :2.25-2.48 (4H,m) , 2.66 (IH,dd,J=7.5, 11.7) , 2.84 (IH,d, J=16.0) ,3.25-3.43 (2H,m) ,3.48-3.64 (lH,m) ,2.97 (IH,dd,J=6.5, 11.8) , 4.31-4.44 (lH,m) ,4.98-5.14 (2H,m) ,5.33 (lH,c,J=2.6) , 5.75-5.92 (2H,m) . IR(Neat,cm~l) :3275, 1776, 1634. Example 20 (3RS.5RS) -3- rN-Propvnovlaminomethyl1 -4-oxa-l-azabicvclof3.2.01 heotan-7-one (20) According to the same method as described in example 19 , the title compound waε obtained aε εolid. Yield:29%. m.p. :76.5-78°C. !H NMR(CDCl3,δ) :2.68 (IH,dd,J=5.3 , 10.0) ,2.67 (IH, ε) ,2.88 (IH,d, J=10.5) ,3.33(lH,d,J=ll.l) , 3.63 (IH,ddd,J=2.6, 4.7 , 10.0) ,3.99(lH,dd, J=4.7,7.9) ,4.36-4.44 (lH,m) , 5.35 (IH,d,J=2.1) , 6.20 (lH,br.ε) . IRjNujol^m "1 ) : 3270,2115, 1782, 1657. Example 21 (3RS.5RS) -3- [N- (Benzyloxycarbonyl) aminomethyl' -4-oxa-l-azabicvclc .3.2.01hentan-7-one (21) According to the same method as described in example 1, the title compound was obtained as oil. Yield:54% -H NMR(CDCl3,δ) :2.70 (IH,dd,J=6.6, 12.3) , 2.66 (IH, ,J=16.6) ,3.22- 3.55(lH,m) ,3.96(lH,dd,J=5.1,11.5) , 4.31-4. 7 (lH,m) , 5.06 (IH,br. s) , 5.10(2H,ε) ,5.31(lH,d,J=2.6) . Example 22 (3RS.5RS) -3-aminomethyl-4-oxa-l-azabicvclc [3.2.01heotan-7-one hvdrochloride (22) 890mg of (3RS, 5RS) -3-Azidomethyl-4-oxa-l-azabicyclo[3.2.0]heptan- 7-one waε hydrogenated as described in example 1. After removal of catalyst by filtration, 5ml cf 1N-HC1 waε added at -78°C. Water layer was then separated ana lyophiiized. The title compound was obtained as pale yellow solid. Yield:73% !H NMR(CDCl3,δ) :2.78-2.86 (lH,m) , 2.82 (IH, ,J=15.6) ,2.98 (IH,d,J= 6.12) ,3.99(lH,d,J=15.6) ,3.87 (IH, dd,J=6.7 , 11.7) ,4.54-4.57 (lH,m) , 5.38(lH,dd,J= 2.6) ,8.38 (2H,br.ε) . IR(Nujol,cm-l) :3325, 1806, 1775. E le 23 (3RS.5RS) -3- (N-Benzovlaminomethvl) -4-oxa-l-azabicvclo.3.2.01 heotan-7-one (23) According to the εame method aε deεcribed in example 1, the title compound was obtained as solid. Yield:53% m.p. :115-116°C -H NMR(CDCl3,δ) :2.76 (IH, d,J=7.Ξ , 11.6) , 2. Sc (IK, a,J=16.7) , 3.31 (IH, dd,J= 2.9,16.8) ,3.49-3.62 (lH,m) , 2.78 (IH,ddd, =2.5 , 6.4 , 14.2) ,4.02 (lH,dd,J= 6.4,11.8) , 4.42- .55 (iK,m) , 5.36 (IK, a, =2.7) ,6.46(1H, br.s) ,7.40-7.57 (3H,m) ,7.7 -7.61 (2H,m) . IR(Nujol,cm _1 ) :3335,1779,1639,1534. Anal. : calcd: C, 62.40; H, 5.72; K, 11.35 Found: C, 62.39; K, 5.69; K, 11.25 Example 2 (3RS, 5RS) -3- [N- (4-Hvdroxvbenzcv ) aminomethv l -4-oxa-l-azabicvclo r3.2.01heptan-7-one (24) According to the same method as described in example 19 , the title compound waε obtained as oil. Yield:25% 1 H NMR(CDCl3,δ) :2.76 (IH,da,J=6.2,11.5) ,2.79 (IH, a,J=16.3) ,3.29- 3.55 (3H,m) ,3.80 (IH,dd,J=6.6, 11.5) , 4.41-4.47 (lH,m) , 5.33 (IH, d,J= 2.5) ,6.79(2H,d,J=8.7) ,7.72 (2H, ,J=8.7) , 8.37 (lH,br.s) , 9.97 (IH, s) . πKNujol,™- 1 ) :3315, 1765, 1600. Example 25 (3RS.5RS) -3- .N- (4-Methoxvbenzovl) aminomethvll -4-oxa-l-azabicvclo ■3.2.01hep an-7-nne (25) According to the same method aε described in example 1, the title compound was obtained as solid. Yield:23% m.p. :142.6°C ~ ~ X H NMR(CDCl3,δ) :2.75 (IH, dd, =7.4,12.2) , 2. E6 (IH, d,J=16.7) ,3.31 (IK, dd,J= 2.9,15.9) ,3.47-3.6 (lH,m) ,3.76 (IH, dd,J=3.5 , 6.4, 14.3) , 3.86 (3H,s) ,4.01(l,dd,J=6.5,11.9) , . 2- .54 (lK,m) ,5.25 (lH,d,J=2.7) , 6.35 (lH.br.s) , 6.94 (2H,d,J=6.8) , 7.75 (2H, d,C=8.9) . IR(Nujol,cm -1 ) : 3290, 177 , 1625. Example 26 (3RS.5RS) -3- [N- (3.4-Dime hoxybenzoy ) aminomethyl " -4-oxa-l- azabicyclo[3.2.01heptan- "7 -one (26) According to the same method as described in example 1, the title compound was obtained as solid. Yield:45% m.p. :170-190°C(dec. ) . X H NMR(CDCl3,δ) :2.76 (IH,dd,J=7. C , 11.8) , 2.69 (IH, a,J=9.1) ,2.32 (IH, dd,J=2.4, 16,5) ,3.52-3.81 (2H,m) ,3.93 (3H,ε) ,3.94 (3H,ε) ,4.02 (lH,dd, J=6.5,11.9) ,4.46- 4.51(lK,m) , 5.36 (lH,d,J=2.6) , 6.38 (lH,br.ε) ,6.87 (lH,d,J=8.3) ,7.26-7.31(lK,m) ,7.43 (IH,d, =2.0) . IRfNujol^m "1 ) :3370, 2850, 1794, 1774 Example 27 (3RS.5RS) -3- ΓN- (4-Flnorobenzovl) aminomethyll -4-oxa-l-azabicvclo r3.2.01heptan-7-one (27) According to the same method aε deεcribec in example 1, the title compound waε obtained aε oil. Yield:16% H NMR(CDCl3,δ) :2.67 (IK,dd,J=6. , 11.7) ,2.76 (IH, a,J=16.6) ,3.23(1H, dd,J=2.8,16.6) , 3.39-3.52 (2K,m) ,2.67 (IH,ddd, =3.7 , 6.3 , 14.3) , 3.93 (lH,dd,J= 6.5,11.9) ,4.35-4.46 (lH,m) ,5.28 (lH,d,J=2.6) ,6.56 (IH, br.s) ,7.00-7.08 (2H,m) ,7.69 (2H,m) . IRWujol.crrT 1 ) =3345,2965,1783 Example 28 (3RS.5R5) -3- ΓN- (2.5-Difluorobenzovl)aminomethvll -4-oxa-l- azabicvclo[3.2.01heptan-^-one (26) According to the same method as deεcribe in example 1, the title compound waε obtained as solid. Yield:43% m.p. :88.8-90.7°C X H NMR(CDCl3,δ) :2.76 (IH,dd,J=7. , 11.8) , 2.67 (IH, a,J=16.4) ,3.32(1H, dd,J=2.6,16.3) , 3.57-3.80 (2H,m) , 4.02 (IH,da, =6.5, 11.8) ,4.44-4.56 (lH,m) ,5.38(lH,d,J=2.6) ,7.06-7.20 (3H,m) ,7.42 (lH,m) . IR(Nujol,cm _1 ) :3340,2935, 1776 Anal. : calcd: C, 55.30; K, 4.29; K, 9.93 Found: C, 55.05; K, 4.18; N, 9.74 Example 29 (3RS.5RS) -3- [N- (2 , 4.5-Trifluorobenzoyl)aminomethyll -4-oxa-l- azabicvclor .2.01heotan-7-one (29) According to the same method as described in example 1, the title compound was obtained as solid. Yield:45% m.p. :76.5-78.1°C ! H NMR(CDCl3,δ) :2.74 (IH,da,J=7.1, 11.8) ,2.87 (IH, d,J=16.3) ,3.33 (IH, d,J=16.4) ,3.57-3.72(2H,m) , 4.02 (IK,dd,J=6. Ξ, 11.8) , 4.67-4.52 (lH,m) , 5.38 (lH,s) ,6.96-7.09(2H,m) ,7.89-8.02 (lH,ιr.) . IRtNujol,™ -1 ) :3300,2960, 1786 Anal. : calcd: C, 52.01; K, 3.69; N, 9.33 Found: C, 51.79; K, 3.49; N, 9.15 Example 30 (3RS.5RS) -3- [N- (4-Cvanobenzoyl)aminomethyl' -4-oxa-l-azabicvclo r3.2.01hentan-7-one (30) According to the εame method as described in example 1, the title compound was obtained as solid. Yield:45% m.p. :130-170°C(dec. ) . -K NMR(CDCl3,δ) :2.74(IH,dd,J=6.9,11.8) ,2.66(IH,a,J=16.5) ,3.26(1H, dd,J=2.9,16.7) ,3.46-3.60(lH,m) ,3.80(IH,dad,J=3.5, 6.4,14.2) ,4.03 (lH,dd,J=6.4,11.8) ,4.43-4.55(lH,m) ,5.37 (IK,d,J=2.6) ,6.65(1H, br.s) ,7.75(2H,d,J=8.5) ,7.90(2H,d,J=8.5) . IRWujol.cπT 1 ) :3360,2235,1795,1774 Example 31 (3RS.5RS)-3-TN- (Thiophen-2-yl)acetylamincmethyll-4-oxa-l- azabicvclo[3.2.01heptan-7-one (3 ) 500mg (3.0mmol) of (3RS,5RS)-3-Azidomethyl-4-oxa-l-azabicyclo [3.2.0]heptan-7-one was hydrogenated as usual manner. The solution was filtered through celite and to the filtrate 0.8ml of Triethylamine was added. Under cooling, Thiophen-2-yl-acetyl chloride, prepared from l.Og of Thiophen-2-yl-acetic acid, waε slowly added to it and the mixture waε stirred at room temperature for 1 hr. The solution was filtered through εilica gel column and the title compound was obtained as oil. Yield:18%. !H NMR(CDCl3,δ) :2.63 (IH,dd,J=7.26) ,2.80 (IK,d,J=16.2) ,3.26(lH,dd, J=2.3,16.0) ,3.40-3.48 (2H,m) ,3.81 (2H,ε) ,2.92 (IH,dd,J=6.5,11.9) , 4.28-4.40 (IH, ) ,5.16(IH, ,J=2.6) ,5.88(IK,br.s) ,6.93-7.04 (2H,m) ,7.23-7.30(lH,m) . IR(Nuj01,01--!) :3310, 1767, 1644. Example 32 (3RS.5RS) -3-[N- [ (Pyridin-3-yl)carbonyllaminomethyl -4-oxa-l- azabicvclo[3.2.01heotan- ~ -one (32) According to the same method as described in example 19, the title compound was obtained aε oil. Yield:7%. -E NMR(CDCl3,δ) :2.75 (IH, d,J=6. ,11.8) ,2.88(IH,a,J=16.2) ,3.34 (lH,dd,J=2.2,16.0) ,3.46-3.63 (IK,m) ,3.82 (IK,ddd,J=3.4, 6.4,14.3) , 4.04(IH, dd,J=6.4,11.9) ,4.44-4.56(lH,m) ,5.38 (IH, ,J=2.7) ,6.54 (lH,br.ε) , 7.43 (IH,dd,J=4.8,7.7) ,8.15(IH,dt,J=2.2,7.8) ,8.78 (lH,dd,J=1.6,4.9) ,9.00(lK,d,J=1.8) . Example (3RS.5RS) -3-[N- (N.N-Diethylcarbamyl) 1aminomethyl-4-oxa-l- azabicvclo[3.2.01heptan- -one( ) According to the same method as described in example 1, the title compound was obtained aε oil. Yield:31% *H NMR(CDCl3,δ) :1.13 (6H, ,J=7.2) ,2.72 (IH,dd,J=7.2,12.0) ,2.84 (IH, dd,J= 16.0) ,3.21-3.34(3H,m) ,3,54 (lH.ddd,J=3.6,6.3,1.3) ,3.95(1H, dd,J=6.5,11.8) ,4.34-4.46(lH,m) ,4.46(lH,br.ε) ,5.31(IH,d,J=2.7) . IR(Neat,cm -1 ) :3355,1780,1629. E mple 3 N.N l -Di- r(3RS.5RS)-7-oxc-l-aza-4-oxabicvclor3.2.01hept-3- YiUmethyl)urea (34) A solution of 340mg(2.Ommcl) of (3RS, 5RS)-3-Azidomethyl-4-oxa-l- azabicyclo[3.2.0]heptan-7-one in 30ml of Ethyl acetate was hydrogenated under 45pεi with 150 mg of 10% Palladium on activated carbon. The reaction mixture was filtered through celite and concentrated to 15ml at cool temperature. At this point, Phosgene (20% in Toluene, 260mg, 2 mmol) was added dropwise while the pH of the mixture was kept at 7 by adding cf a saturated solution of K 2 CO 3 . The reaction mixture was extracted with Ethyl acetate (3 x 20ml) and the combined organic layer was dried over MgS04 an ^ concentrated. Crystallization of the yellow residue in Ethyl acetate gave product as a white priεmε . Yield:42% m.p.: >300°C(dec.) 1 H NMR (DMSO-d 6 ,δ) : 2.64 (2K,dd,J=6.4, 11.5) ,2.77 (2K,d,J=16.3 ) ,3.18 (4H,t,J=5.4) ,3.33(2H,dd,J=2.7,16.2) , 4.22-4.34 (2K, ) , 5.30 (2H, d, J=2.6) ,6.15(2H,br.t,J=5.8) . IR(Nujol,cm -1) :3320,1770. Example 35 (3RS.5RS) -3- (N-Methaneεul onylaminomethyl^ -4-oxa-l-azabicvclo [3.2.01heptan-7-one (35) According to the same method as described in example 1, the title compound waε obtained aε εolia. Yield:23% m. p . : 115-190°C ( gradually dec ) . *H NMR (CDCl3 , δ) : 2 . 82 ( IH, dd, J=7 . 5 , 11 . 8 ) , 2 . 87 ( IH, a, J=16 . 8 ) , 3 . 01 (3H,s) ,3.22-3.67 (3H,m) , 4.00 (IH,dd,J=6.4, 11.9) ,4.35-4.49 (lH,m) , 4.58 (IH,br.s) ,5.35(lH,d,J=2.8) . IR(Nujol,cm -1 ) :3235, 1783, 1302. Example 36 (3RS.5RS) -3- fN- (4-Toluenesulfonvl) aminomethvll -4-oxa-l-azabicvcln [3.2,Q1heptan-7-pne (36) According to the same method as described in example 1, the title compound was obtained as solid. Yield:74% - ._./ - *H NMR(CDCl3,δ) :2.44(3H,s) ,2.74(lH,dd,J=11.6,7.4),2.81(lH,d,J= 15.8) ,2.97-3.21 (2H,m) ,3.2c (IK, dd,J=2.2 , 15.9 ) , ? .01(lH,dd,J=11.9, 17.3) ,4.26-4.38 (lH,m) ,4.75 (lH,b . t,J=6.3) ,5. 2 -. (lH,d,J=2.6) ,7.33 (2H,d,J=8.1) ,7.74(2H,d,J=5.3) . IR(Nujol,cm "1 ) :3275, 1778, 1153. Example 27 (3RS.5RS) -3- [N- (4-Chlorobenzenesulfonyl) aminomethyll -4-oxa-l- azabicvclof3.2.01heptan-7-one (37) According to the same method as described in example 1, the title compound waε obtained aε solid. Yield:35% m.p. :101-103°C X H NMR(CDCl3,δ) :2.78 (IH,dd,J=7.4, 11.6) , 2.62 (IH, a,J=15.9) ,2/99- 3.23 (2H,m) ,3.29 (IH,dd,J=6.5, 11.9) ,4.28-4.40 (lH,m) ,4.89 (lH,br. , J=6.4) ,5.27(lH,d,J=2.7) , 7.51 (2K, d,J=8.7) , 7.80 (2H,d,J=8.7) . IR(Nujol,cm _1 ) :3275, 1764, 1320, 1153. Ex mple 8 (3RS.5RS)-3-N-(4-Methoxvbenzenesulfonvl)aminomethvl-4-oxa-l- azabicvclor3.2.01heotan-7-one (38) According to the same method as deεcribed in example 1, the title compound was obtained as εolid. Yield: 66% m.p. : 116.5-118.5°C 1 H NMR(CDCl3,δ) :2.79 (IH,da,J=5.2 , 7.3 ) ,2.80 (IH,a,J=16.0) ,2.96-3.21 (3H,m) ,3.27(lH,dd,J=2.5,16.7) , 3.88 (3H, ε) , 3.92 (IH,dd,J=5.4, 6.5) , 4.24-4.38 (lH,m) ,4.89 (lH,br.t,J=6.4) , 5.26 (IH, d,J=2.6) , 6.99 (2H,d,J= 8.9) ,7.79(2H,d,J=8.9) . πUNujol.cπr 1 ) : 3265, 1770, 1578, 1489. Example 3 Q (3RS , 5SR) -3- (Aminoacetyl) oxymethyl-4-oxa-l-azabicvclo . .2. Qi heotan-7-one hvdrochloride (39) Azidoacetate (390mg) obtained in example 5, 5% Palladium on activated carbon(390mg) were shaken in Ethanol-Ethyl acetate(10ml each) at 50psi for lhr. After the reacticr., catalyεt was removed by filtration and 1.5ml of 1N-HC1 waε added under cooling condition. Reaction mixture was purified cr. HP-20 column by elution with water. The title compound was obtained after lyophylization as εolid. Yield:46% m.p. :85-110°C(dec. ) . 3-H NMR (DMSO-d 6 ,δ) :2.76-2.88(lH,m) , 2.80 (iK.a,J=16.3) , 2.98 (IH,d,J= 9.4) ,3.83-4.28 (6H,m) ,4.57-4.83 (lH,m) , 5.36 { H,a,J=2.5) ,7.66(1H, br.ε) ,8.43 (2H,br.ε) . IR(Nujol, cm "1 ) :3205, 1751, 1651. Example 40 (3RS.5SR) -3- fN- (N-Benzvloxvcarbonvlaminoacetvl) aminoacetvl1 oxvmethvl-4-oxa-l-azabicvcior3.2.01hentar-7-one (40A) and (3RS.5SR) -3- fN- (Aminoacetvl) aminoacetvl1oxvmethvl-4-oxa-l- azabicvclof3.2.01heotan-7-one hvdrochloride(40B) a mixture of (3RΞ, 5SR) -3-Bromomethyl-4-oxa-l-azabicyclo[3.2.0] heptan-7-one (2.Og, 9.71mmol) , N- (benzyloxycarbonylaminoacetyl) aminoacetic acid (2.6g, 11.6mmol) and Cesium carbonate (3.8g, ll.δmmol) in Hexamethylphosphoric triamide (15ml) was stirred at 65-70°C for 2hrs. Reεulting mixture waε poured onto ice-water and extracted with Ethyl acetate. The extract waε washed with water, brine and dried over Magneεium sulfate. Solvent was removed in vacuo and the residue was purified on a silica gel column chromatography using ethyl acetate-acetone (9:1) as the eluent gave an off-white solid (40A) . Yield:3.0g (79%) . !H NMR (DMSO-d6,δ) : 2.70-2.55 (2K,m) , 3.30 (IK, dd,J=16.1 , 2.4) , 3.62-4.20 (7H,m),4.48-4.62(lH,m) ,5.11(2H,ε) ,5.32 (lK.d,J=2.5) ,7.35{5H,s) ,7.55 (lH,t,J=5.7) , 8.35(1H, ,J=5.7) . :3461, 3405, 1781, 1750, 1722, 636. N- (benzyloxycarbonylaminoacetyl) aminoacetoxy derivative, obtained above (l.lg, 2. δlmmol) and Palladium on activated carbon (10%, 53.8% moist, 0.8 g) in Methanol (140ml) was shaken at 50psi for lhr under Hydrogen atmosphere. After the reaction, catalyst was filtered off using celite pad. The filtrate waε concentrated in vacuo and the residue was re-dissolved in Ethyl acetate. Acidification (IN HCl 2.4ml, water 26ml) and followed by freeze- drying gave a solid (40B) . Yield: 660mg(80%) . ^ ■ H NMR(DMSO-ds,δ) : 2.62-2.76 (2H,m) , 3.10-4.14 (8H,m) , 4.44-4.55 (lH,m) , 5 . 27 ( lH, d , J=2 . 5Hz ) , 8 . 23 ( 3H . br . ε ) , 8 . 94 ( IH , , J=5 . 7 ) . IR dJUj o^ cπT 1 ) : 3125 , 1774 , 1748 , 1682 , 1618 . Example 41 (3RS.5SR) -3- ■N-(L-2-N-Benzvloxvcarbonvlaminopropanovl)aminoacetvl] oxvmethvl-4-oxa-l-azabicvcloT3.2.Olhentar.-7-one(41A) and (3RS .5SR) -3- rN-(L-2-Aminopropanovl)aminoacetvl1 oxvmethvi-4-oxa-l-azabicvclo r .2.01heptan-7-one hvdrochloride(41B) A solution of (3RS,5SR) -3- [N- (Benzyloxycarbonyl)aminoacetyl] oxymethyl-4-oxa-l-azabicyclo[3.2.0]heptan-7-one (1.0g,2.99mmol) in Ethyl acetate (80ml) was hydrogenolysed at 50psi for 15 hr using Palladium on activated carbon (10%, 53.8% moist, 0.75g) . The mixture was filtered through a pad of celite and cooled in an ice-bath. 1,3-Dicyclohexyl carbodiimide (740mg,3.56mmol) was added to an ice-cold solution of N-Benzyloxycarbonyl- -2-aminopropanoic acid (1.33g, 5.98mmol) in Ethyl acetate (80ml) and the mixture waε stirred for 1 hr. The N-Benzyloxycarbonyl-L-2-aminopropanoic acid- DCC complex was added to the ice-cold Ethyl acetate solution of the amine prepared above. The mixture was stirred at room temperature overnight. The precipitated urea was filtered off, the filtrate was concentrated and paεεed through a silica gel column using Ethyl acetate as the eluent to give a thick oil (41A) . Yield: 83% -H NMR(CDCl3,δ) : 1.40 (3H, a, =7.1) ,2.74-2.88(2H,m) ,3.29 (IH,dd,J=16.4, 2.7) ,3.93-4.60(6H,m) ,5.11(2H,m) , 5.29-5.41 (2H,m) , 5.35 (lH,br.ε) , 6.74(lH,br.ε) ,7.34(5H,s) . (N-protected aminopropanoyi)aminoacetyl ester derivative was deprotected as same manner described in example 40 and the title compound(41B) waε obtained aε εolid. Yield:720mg(63%) . -ϋ NMR(DMSO-dg,δ) :1.41(3K,a,J=6.9) , 2.75-2.83 (2H,m) , 3.31 (IH) , 3.81- 4.24 (6H,m) ,4.55 (lH,m) , 5.34 (IH, , =2.4) , 8.39 (3H,b . ε) , 9.12 (lH,br.ε) IR(Nujol,cm " l) :3345,3195, 770, 1746, 1681. Example 42 (3RS.5SR)-3-fN- (L-2-N-Benzvloxvcarbonvlamino-3-methvlbutvrvl) aminoacetvlloxvmethvl-4-oxa-l-azabicvclor3.2.01hepr_an-7-one (42A) and (3RS.5SR) -3-.N-(L-2-Amino-3-methvlbutvrvl) minoacetvl! oxvmethvl-4-oxa-l-azabicvcio[3.2.01heptan-7-one hvdrochloride (42B) According to the same manner described as in example 40, L-(2-N- benzyloxycarbonylamino-3-me hylbutyl)aminoacetic acid was coupled with (3RS, 5SR) -3-Bromomethyl-4-oxa-l-azabicyclo[3.2.0]heptan-7-one and N-protected title compound(42A) waε obtained as off-white solid. Yield: 83% m.p. :143-145°C. λ E NMR(CDCl3,δ) :0.94 (3H, a,J=6.8) , 0.98 (3H,a,J=6.8) ,2.09-2.25 (lH,m) , 2.73-2.88 (2H,m) ,3.29 (IH,da,J=16.3 ,2.4) , 3.93-4.31 (7H,m) ,4.48-4.59 (lH,m) ,5.11 (2H,s) ,5.32-5.40 (2K,m) ,6.58 (lK,br.t) ,7.34 (5H,s) . IR(Nujol,cm _1 ) :3295, 1793, 1750, 1687, 1647, 1619. The benzyloxycarbonyl group waε hydrogenciyεed according to the εame manner deεcribed as in example 40, the title compound(42B) waε obtained as solid. Yield:64% !H NMR(DMSO-d 6 ,δ) :0.91(6H,d,J=6.8) ,2.02-2.12 (IH,m) ,2.66-2.76(2H,m) , 3.29(lH,dd,J=16.1,2.6) ,3.62-4.17 (6H,m) ,4.40-4.55(lH,m) , 5.26(IH,d, J=2.5) ,8.25(3H,br.ε) , 9.0(lK,br. ) . IRmujol,™- 1 ) :3195,1776,1750,1677,1621. Example 43 (3RS.5RS) -3-N-(Aminoacetvl)aminomethyl- -cxa-1-azabicvclof3.2.01 heptan-7-one hvdrochloride(43) Azidoacetamide compound, obtained in reference example 9 (890mg) , waε hydrogenated in preεence of 5% Palladium on activated carbon(80Omg) in Ethanol(20ml) at 50pεi fcr 2hr. After removal of catalyst by filtration, 3.0ml of 1N-HC1 was added under cooling condition and solvent was removed in vacuo. The residue was purified on HP-20 column using water as eluent and the title compound was obtained after lyophilization as yellow solid(43). Yield:62% *H NMR(CDCl3,δ) :2.70(IH,dd,J=6.5,11, 6) ,2.79 (IH,a,J=16.4) ,3.19- 3.44(3H,m) ,3.56 (2H,s) ,3.82 (IH,dd,J=6.5,1 .6) ,4.27-4.39 (lH,m) , 5.34 (lH,d,J=2.6) ,8.21(2H,br.s) ,8.72(lH,br. ,J=5.6) . IR(Nujol,cm _1 ) :3255,1768 Ex mple 44 ( 3RS . 5RS ) -3 -N- (N-Acetvlaminoacetvl ) aminomethvl-4-oxa-l-azabicvclo r 3 . 2 . 01 heptan-7 -one ( 44 ) Acetic anhydride (17ml) was added dropwiεe at 5°C to a mixture of N- (aminoacetyl) aminomethyl amido derivative which was obtained in example 45(20mg) and Triethylamine (17mg) n Dichloromethane(lml) . The mixture was stirred under εame condition for 30 minuteε. The reaction mixture waε purified by εilicagel column chromatography uεing Acetone aε eluent and title compound (44) waε obtained aε white solid. Yield:44% m.p.l42-144°C X H NMR(CDCl3,δ) : 1.85 (3H,s) , 2.66 (IH,dd,J=6. ,11.5) , 2.78 (IH,d,J= 16.3) ,3.14-3.38 (3H,m) , .65 (2H,a,J=10.2) , .77 (IH,dd,J=6.6, 11.6) , 4.24-4.36 (lH,m) , 5.30 (IH,d,J=2.6) ,7.99-8.06 (2H,m) . IR(Nujol,cm _1 ) :3280, 3095, 1800, 1636 Example 45 (3RS.5RS) -3- TN- (L-2-N-Benzvloxvcarbonvlammo-3-methvlbutvrvl) aminoacetvl1aminomethvl-4-oxa-l-azabicvcic r 3.2.01heotan-7-one (45A) and (3RS .5RS) -3- .N- ( -2-amino-3-me vlbutvrvl)aminoacetvl1- aminomethvl-4-oxa-l-azabicvclo.2.2.01heptan-7-one hvdrochloride( B) According to the same manner as described in example 41, N- protected title compound(45A) waε obtained aε solid. Yield:23% 3-H NMR (DMSO-de.δ) :0.79(6K,m) ,1.84-1.94 (lK,m) , 2.54-2.74 (2H,m) ,3.15- 3.29 (5H,m) ,3.62-3.80(3H,m) , 4.19-4.25 (lH,m) ,4.96 (2H,s) , 5.23 (IH,d, J=2.6) ,7.29 (5H,s) ,7.82 (lH,br.t) ,8.15 (lH,br.s) . N-protected amide derivative, obtained aε above, was deprotected as same manner described in example 43 and the title compound(45B) was obtained as solid. Yield:0.40g(70%) . -H NMR(DMSO-dg,δ) :0.80(6H,m) , 1.94-2.10 (lH,m) , 2.60-2.74 (2H,m) ,3.05- 3 . 35 ( 5H , m ) , 3 . 50 - 3 . 80 ( 3H , m) , 5 . 25 ( IH , a , J=2 . ) , 8 . 35 ( 3H , br . s ) , 8 . 80 ( lH , br . s ) . IR(Nujol, cm "1 ) : 3195, 1782, 1718, 1678, 1653, 1603. Example 46 (3RS.5RS) -3-N- fN- (L-2-N-Benzvioxycarbonylamino-3-carboxypropanoyl) aminoacetyl!aminometh y l-4-oxa-l-azabicvclo [3.2.01heptan-7-one (46A) and (3RS .5RS) -3-N- fN- (L-2-Amino-3-carboxypropanoyl) aminoacetvl1 aminometh y l -4-oxa-l-azabicvclc F3.2.01heotan-7-one (46B) Azidoacetamide derivative of clavam compound, obtained in example 43, was coupled with protected aεpartic acid derivative using similar method described in example 41 and the protected title compound was obtained (46A) . Yield:42%. X H NMR(CDCl3,δ) :2.62-2.88 (2H,m) , 2.76 (IH,a,J=16.6) , 3.10-3.25 (2H,m) , 3.34-3.42 (2H,m) , 3.77-4.17 (3H,m) , 4.30-4.40 (lH,m) , 4.53-4.63 (lH,m) , 5.13 (4H,s) ,5.28(lH,t,J=3.1) , 5.75-5.82 (lκ,m) , 6.67-6.75 (lH,m) , 6.94- 7.03 (lH,m) ,7.30-7.38(10H,m) . 0.5g of the protected compound and 0.5g of 5% Palladium on activated carbon in 10 ml Methanol waε stirred under Hydrogen atmosphere at normal pressure for 2.5hrs. Later the catalyst was filtered through celite and filtrate was concentrated. Acetonitrile was added to the residue and solid was collected by filtration(46B) . Yield:34%. m.p. :200°C(dec. ) . E NMR(CDCl3,δ) : 2.64-2.69 (lH,m) ,2.77 (IH, a,J=16.2) , 3.08-3.85 (6H,m) , 3.32(lH,d,J=13.4) , 4.25-4.38 (lK,m) , 5.30 (IH,d,J=2.4) ,8.25-8.56 (2H,m) . IR(Nujol, cm -1 ) :3290,1773. Example 47 f 3 . 2 . 01 hep an-7 -one ( 47B ) (3RS, 5RS) -3- [N- (Azidoacetyl)aminomethyl] -4-oxa-l-azabicyclo [3.2.0]heptan-7-one, obtained in example 43, waε coupled with [4- (Benzyloxycarbonylamino) -4-benzyloxycarbcnyl]butyric acid uεing similar method described in example 41 and the protected title compound was obtained(47A) . Yield:21%. m.p. :45°C H NMR(CDCl3,δ) :1.97-2.19 (lK,m) , 2.17-2.3C ilH.m) , Z .40-2.50 (2H,m) , 2.73(lH,dd,J=6.1,11.6) , 3.25 (IH,a,J=16.2) ,2.94 (IK, dd,J=6.9, 11, 6) , 4.05-4.20 (4H,m) , .40-4.55 (2H,m) , 5.10 (2H,m) , 5.17 (2H,m) ,5.30 (IH, t, J=2.8) ,5.39 (br.s) ,7.35 (10K,s) . IR(Neat, cm "1 ) :3355, 1784, 1739 , 152 . This protected amide derivative obtained above was deprotected by using same method as described in example 46 and the title compound(47B) was obtained as εolid. Yield:10%. m.p. :220°C(dec) *H NMR(DMSO-d 6 ,δ) :1.90-2.04 (2H,m) ,2.75 (IK,dd,J=5.9,16.4) ,2.50-2.57 (4H,m) ,2.80(lH,d,J=16.4) ,2.33 (IK,dd,J=2.6 , 16.2) , 3.84 (IH,dd,J=7.1, 11.6) ,4.12(2H,d,J=3.4) ,4.22-4.47 (lH,m) , 5.29 (IH, ,J=2.5) ,5.08-5.21 (lH,br.d) . Example 46 (3RS.5SR)-3-(L-2-N-Benzvioxvcarbonvlaminopropanovl)oxvmethvl-4- oxa-1-azabicvclon .2. Olheotan-7-one (48A) and (3R5.5SR) -3- (L-2- A inopropanovl . oxvmethvl-4-oxa- ' -azahicvcl- ' 3.2 . C1heptar.-7-one hvdrochloride (48B) According to the same method described in example 40, N-protected title compound(48A) waε obtained aε oil. Yield: 68%. l E NMR(CDCl3,δ) : 1.44 (3H, a, =7.2} , 2.70-2.67 (IH,m) , 2.84 (IH,a,J= 16.2) ,3.27(lH,d,J=16.0) ,2.98 (IK, dd,J=7.1, 1.6) ,4.18-4.56 (2H,m) , 4.30-4.65 (2H,m) ,5.12 (2H,s) ,5.16-5.30 (lH,ιr. > ,5.32 (lH,ε) ,7.35 (5H,s) . IR(Neat, cm "1 ) :3550, 1784, 1748, 1720 , 1525. The N-protected ester derivative obtained above was deprotected by catalytic hydrogenation in Methanol using 5% Palladium on activated carbon at room temperature (50pεi, lhr) . After removal of catalyst, 1N-HC1 (1.7ml) was added to the reaction mixture under cooling condition and resulted solution waε then purified by HP-20 column chromatography using water aε eluent. The title compound(48B) waε obtained after lyophilization aε solid. Yield:15% IH NMR(DMSO-d 6 ,δ) : 1.43 (3K,a,J=7.4) , 2.70-2.59 (lH,m) ,2.80 (IH,d,J= 15.7) ,3.26-3.40(lH,m) , 3.88 (IH,dd,J=6.7 , 11.6) ,4.02-4.3 (4H,m) , 4.50-4.70 (lH,m) ,5.35 (lH,t,J=3.4) ,8.57 (2K,br.s) . IR(Nujol,cm _1 ) :3400,3275, 1746. xampl 9 (3RS.5SR)-3-r -2-N-(L-2-N-Benzvloxvcarbonviamino-3-methylbutyryl) aminopropanovl1oxvmethvl-4-oxa-l-azabicvclof3.2.01heptan-7-one (49A) and (3RS .5SR) -3- fL-2-N- (L-2-Amino-2-methvlbutvrvl) amino propanovlloxvmethvl-4-oxa-l-azabicvclor3.2.01heptan-7-one hvdrochloride(49B) According to the same method aε descried in example 41, protected title comoound(49A) was obtained as an cff-white solid. Yield: 65% m.p. :167-169°C. 1 H NMR (CDCl3,δ ) :0.93(3H,d,J=6.8) ,C.98(3K,d,J=6.S) ,1.35(3H,d,J= 2.04-2.18 (lH,m) ,2.73-2.89 (2K,m) ,3.33 (IH, d,J=16.2, 2.6) ,3.94-4.03 (2H,m) ,4.21-4.25 (2H,m) ,4.55-4.62 (2H,m) ,5.11 (2H,s) ,5.35 (2H,br.ε) , 6.30 (IH,d,J=7.1) ,7.35 (5H,s) . IR (Nujol,cm _1 ) -.3315, 1785, 1746, 1688, 1648. The N-protected ester derivative was deprotected as same manner described in example 40 and the title compound(49B) was obtained as solid. Yield: 66%. NMR(DMSO-de,δ) : 0.98 (6H,a,J=6.8) , 1.35 (3K,a,J=7.2 ) , 2.12-2.15 (IH, ) ,2.75-2.83 (2H,m) , 3.31-3.38 (lH,m) , 3.64-4.56 (6H,m) , 5.35 (IH, d, J= 2.9) ,8.30(3H,br.ε) , 9.06 (lK,br .a, J=6.6) . IRtNujol.cirT 1 ) -.3120,1792,1763,1740,1683,1650. E ample 5Q (3RS.5RS) -3-N- (L-2-N-Benzvloxvcarbonvlaminopropanovl)aminomethvl- 4-oxa-l-azabicvclof3.2.0TnePtan-7-one(50A) and (3RS.5RS) -3-N- (L- 2-Aminopropanovl)aminomethvl-4-oxa-l-azabicvclof .2.Olheotan-7- one hvdrochlorid (50B) According to the same procedure described in example 43, N- protected title compound(50A) was obtained. Yield: 68% X H NMR(CDCl3,δ) :1.42 (3H,d,J=17.3) ,2.72-2.57 (lH,m) , 2.81 (IH,d,u= 16.6) ,3.48(lH,dd,J=3.4,16.6) ,3.33-3.57 (2H,m) ,3.85-4.00 (lH,m) , 4.16-5.76 (2H,m) , 5.12 (2H, s) , 5.26-5.31 (lH,m) , 6.39 (lH,br.ε) , 7.33 (5H,s) . IR(Neat,cm "1 ) :3295, 1782, 1653 , 1533. The title compoun (50B) was obtained after decrctection as described in example 43, followed by salt formation. IK NMR(DMSO-d δ ,δ) : 1.35 (3K, ,J=ό . S) , 2.65-2.70 (IK,m) , 2.79 (IK, dd, = 3.1,16.4) ,3.10-3.50 (3H,m) ,2,77-3.90 (2H,m) ,4.27-5.92 (lH,m) ,5.32 (IH, t,J=3.1) ,8.12 (2H,br.s) ,8.66 (lH,m) . IR(Nujol,cm _1 ) : 3380, 3230, 1774, 1670, 1562. Example (3RS.5SR)-3-(D-2-N-Benzvioxvcarhonvlaminopropanovl)oxvmethvl-4- oxa-l-azabicvclof3.2.01hepr-an-7-one(51A) and (3RS .5SR) -3- (D-2- Aminopropanovl)oxvmethvl-4-c l χa-i-azabicvciof3.2.01heotan-7-one hvdrochloride(5IB) According to the same procedure described in example 43 , N- protected title compound(51A) was obtained aε oil. Yield: 58% *H NMR(CDCl3,δ) : 1.4 (3H,ά,J=7.2) , 2.20-2.56 (lH,m) , 5.68 (IH,d,J= 16.3) ,6.54(lH,d,J=15.9) , 2.97 (IK, dd,J=7. C, 11.6) ,4.17-4.25 (2H,m) , 4.38-4.63 (2H,m) ,5.11 (2H,ε) ,5.25 (lH.br.a,J=6.4) ,5.32 (lH,ε) ,7.35 (5H,m) . IR(Neat,cm "1 ) :3355, 1704, 1748,1720, 1526. The title compound(51B) waε obtained after deprotection aε described in example 43, followed by salt formation. Yield:18% H NMR(DMSO-d 6 ,δ) :2.87 (3H, a,J=7.4) , 2.71-2.92 (2H,m) ,3.30-3.94 (lH,m) ,3.88(lH,dd,J=6.9,11.8) ,3.96-4.44 (3H,m) , .50-4.69 (lH,m) , 5.35 (lH,t,J=3.5) ,8.57 (lH.br.ε) . IR(Nujol,cm _1 ) :3400, 1746, 1645. Example 52 (3RS.5SR) -3-(D-2-N- fD-2-N- (Thiophen-2-yl) acetylaminopropanoyl - aminopropanoyl)oxymethyl-4-ox5-l-azabicv l [3.2. lheptan-7-one (52) According to the similar method aε described in example 40, the title compound(52) was obtained as solid. Yield: m.p. :122-125°C ! H NMR(CDCl3,δ) :1.34 (3H, ,J=7.0) , 1.40 (3H, ,J=7.2) , 2.80 (IK, dd,J= 6.3,17.9) ,2.85(lH,d,J=15.4) ,3.20 (IH,dd,J=2.6, 16.2) ,3.78 (2H,ε) , 3.97 (lH,dd,J=6.7,11.6) , 4.20-4.26 (2H,m) , .47-4.55 (2H,m) , 5.32-5.35 (lH,t,J=3.3) ,6.23 (lH,br.d,J=7.1) ,6.67 (lH,br.d,J=7.1) ,6.95-7.01 (2H,m) ,7.24-7.26(lH,m) . IR(CHCl 3 ,cm-l) :3295, 1778, 1747, 1646. Example 53 (3RS.5RS) -3-N- (D-2-N-Benzyloxycarbonylamir.opropanoyl)aminomethyl- 4-oxa-l-azabicvcloT3.2 ■01heptan-7-one(53A- and (3RS■ 5RS) -3-N- (D- 2-Aminopropanoyl)aminomethyl-4-oxa-l-azabicvclc[3.2.Olheotan-7- one hvdrochloride (53B) According to the same method described in example 43 , N-protected title compound(53A) was obtained as oil. Yield: 60% X H NMR(CDCl3,δ) :1.42 (3H,a,J=17.3) , 2.72-2.57 (lH,m) ,2.81(IH,d,J= 16.6) ,3.48(lH,dd,J=3.3,16.6) , 3.33-3.57 (2K,m) ,3.85-4.00 (lH,m) , 4.16-5.76 (2H,m) , 5.12 (2H, ε) , 5.26-5.31 (lH,m) , 6.39 (lH,br.ε) ,7.33 (5H,ε) . IR(Neat,cm "1 ) :3295, 1782 , 1653 , 1533. The title compound(53B) waε obtained after deprotection as described in example 43, followed by salt formation. Yield:73% IH NMR(DMSO-0 6 ,δ) :1.35 (3K, a,J=6.9) , 2.65-2.70 (IK,m) , 2.79 (IH,dd,J= 3.1,16.4) ,3.10-3.50(3H,m) , 2 ,77-2.90 (2H,m) ,4.27-Ξ .92 (IH,m) , 5.32 (lH,t,J=3.1) ,8.12 (2H,br.s) ,8.65 (lH,br.s) . IRfNujo^cnT 1 ) : 3380, 3230, 1774, 1670,1562. Example 54 (3RS.5SR) -3- (L-2-N-Benzvicxycarbonylaminc-3-methylbutyryl) - oxvmethvl-4-oxa-l-azabicvclor3.2. Olhentar.-7-one (54A) and (3RS.5SR) -3- (L-2-Amino-3-methylbutyryl)oxymethyl-4-oxa-l- azabicvclof3.2.01heotan-7-one hvάrochlcride (54B) According to the same procedure described in example 43, N- protected title compound(54A) waε obtained as oil. Yield:49% ^ ■ H NMR(CDCl3,δ) :0.91(3H,a,J=6.9) , 0.98 (3K, a,J=6.8) , 2.13-2.22 (IK, m) ,2.84(2H,d,J=16.3) , 3.23-2.33 (lK,m) ,4.0C (IK,da,J=7.0, 11.6; ,4.17- 4.33 (3H,m) ,4.50-4.60(lH,m) , 5.11 (2K, ε) , 5.20-5.26 (lH,m) , 5.33 (IH,ε) , 7.36(5H,ε) . The title compound(54B) was obtained after deprotection as described in example 43, followed by salt formation. Yield:71% X H NMR(DMSO-d6,δ) :0.95-0.99 (6H,m) ,2.16-2.22 (lH,m) , 2.76-2.86 (2H,m) ,3.32-3.40(lH,m) ,3.84- .32 (4H,m) ,4.57-4.60 (lH,m) , 5.35 (lH.d, 2.6) ,8.54(3H,br.s) . IRtNujol.cm" 1 ) =1778,1742, 1683, 1646 Example 55 (3RS.5SR) -3- fL-2- (N-Benzvicxvcarbonvlaminoacetvl) amino-3- methvlbutvrvπoxvmet-hvl-4-oxa-l-azabicvclor3.2.01heotan-7-one (55A) and (3RS .5SR) -3- fL-2- (N- minoacetvl) amino-3-methvlbutvrvl ' oxymethyl-4-oxa-1-r_zabicvclcf3.2.01heptan-7-one hvdrochloride (55B) According to the similar method described as i example 40, N- protected title compound (55A) was obtained as oil. Yield:81%. X H NMR(CDCl3,δ) :0.93(3H,d,J=6.6) ,0.95(3K,d,J=6.6) ,2.05-2.28(lK,m) , 2.74-2.90 (2H,m) , 3.30 ( IK, dd,J=1 .1, 2.4) ,2.53-4.05(3H,m) ,4.12-4.35 (2H,m) ,4.48-4.66(2H,m) ,5.11(2H,ε) ,5.34-5.47(2H,br.ε) ,6.50(lH,d, J=8.0) ,7.35(5H,ε) . IRtNujol.cπT 1 ) :3345, 1784, 740, 1680. The title compound(55B) was obtained after deprotection as described in example 40, followed by salt formation. Yield:48%. l i NMR(DMSO-dg,δ) : 0.93 (6H, a,J=6.6) , 2.04-2.14 (lH,m) , 2.76-2.83 (2H,m) , 3.32-3.40(lH,m) , 3.63-4.31 (6H,m) ,4.54-4.5 (lH,m) , 5.33 (IH, dd,J=7.0, 2.5) ,8.82(3H,br.ε) ,8.86 (IK br,s) . IRWujol.cπT 1 ) : 3185, 1779, 1735, 1681. Example 55 (3RS.5SR)-3-[L-2-N-(L-2-N-Benzvloxvcarbonvlamino-3-methvlbutvrvl) amino-3-methvlbutvrvl1oxvmethvl-4-oxa-l azabicvciof3.2.01heptan- 7-one(56A) and (3RS.5SR) -3- fL-2-N- (L-2-Ammo-3-methylbutyryl) - amino-3-methvlbutvrvlloxvmethvl-4-oxa-l-azabicvcio-f3.2.01heptan- 7-one hvdrochloride (56B) According to the εimilar methoa deεcribe in example 41, protected title compound(56A) waε obtained. Yield:77%. H NMR(CDCl3,δ) : 0.89-0.98 (12H,m) , 2.06-2.21 (2H,m) , 2.73-2.88 (2H,m) , 3.29(lH,d,J=16.2) , 3.93-4.25 (4H,m) , 4.52-4.59 (2H,m) , 5.11 (2H, ε) , 5.33 (lH,d,J=2.8) ,5.45(lH,d,J=6.8) , 6.54 (IH, d,J=8.4) ,7.34 (5H,ε) . The title compound(56B) waε obtained after deprotection aε deεcribed in example 40, followed by εalt formation. Yield : 64% . 1 H NMRfDMSO-dgδ) :0.94-l.C: (12K, d,J=6.7) , 2.10-2.2C (2H,m) , 2.71-2.84 (2H,m) ,3.32-3.40 (2H,m) ,3. =0-3.90 (2H,m) ,4.15-4.23 (3H,m) ,4.50-4.10 (lH,m) , 5.34(lH,d,J=2.8) , 5.33 (3K,br.ε) , S .75 (IH, a,J=7.1) . IR(Nujol,cm ~1 ) :3325, 3200,1793, 1762, 1741, 1685, 1653. Example 57 (3RS.5RS) -3-N- fL-2-N- (L-2-N-Benzyloxycarbonylamino-3-benzyloxy- carbonv]propanovl)amino-:--methvlbutvrvlloχymethyl-4-oxa-l- azabicvclo[3.2.01heptan- -one (57A) and (3RS .5RS) -3-N- fL-2-N- (L-2- Amino- -carboxypropanoyl)amino-3-methvlb tyrylloxymethvl-4-oxa-l- azabicvclo[3.2.01heptan-~-one (57B) According to the similar method as described in example 46, protected title compound(Ξ7A) waε obtained. Yield:89%. X H NMR(CDCl3,δ) :0.90(6H, t,J=7.2) , 2.12-2.15 (lH,m) , 2.69-2.88 (3H,m) ,3.08(lH,dd,J=4.2,17.4) , 2.29 (IH, d, =16.4) ,3.92-4.07 (IH, m) ,4.10-4.25(2H,m) , 4.43-4.62 (3H,m) ,5.13 (4K,s) , 5.30-5.34 (lH,m) , 5.97-6.04 (lH.br.d) , 6.95-7.04 (IH,br.d) , 7. 5 (10H,d,J=3.0) . IRfNujol.cirT 1 ) :3425, 1783, 1737, 1679. According to the similar method described as in example 46, the title compound(57B) waε obtained as solid. Yield:72% m.p.l90°C(dec. ) . IH NMR(DMSO-d6,δ) :0.90 (6K,a,J=6.3) ,2.0-2.40 (2H,m) ,2.68-2.85 (2H,m) , 3.16(lH,ε) ,3.25-3.39(lH,m) ,3.63-3.90 (2H,m) ,4.08-4.26 (3H,m) ,4.48- 4.58 (lH,m) ,5.32 (lH,ε) , 8.62-8.75 (lH,m) . IR(Nujol,cm ~ l) : 1783, 1742. Example 55 (3RS.5RS) -3-N- (L-2-N-Benzyloxycarbonylamir.o-3-methylbutyryl) aminomethyl-4-oxa-i-azabicvcio .3.2. OTneptan-7-one (58A) and (3RS.5RS) -3-N- (L-2-Amino-3-me hylbutyryl) aminomethyl-4-oxa-l- azabicvclo f3.2.01heptan-7-one hvdrochloride (58B) According to the εame procedure deεcribed in example 43, N- protected title compound(55A) was obtained as solid. Yield:44% 1 H NMR(CDCl3,δ) : 0.97 (6H,a, =7.0) , 2.15 (IH, q,J=6.8) ,2.58-2.70 (lH,m) ,2.82(lH,d,J=16.6) ,3.28 (IK,d,J=16.3) ,3.34-3.62 (2H,m) , 3.36- 4.01(2H,m) ,4.25-4.41(lH,m) , 5.12 (2H, d,J=l.4) , 5.28 (IH,d,J=2.5) ,5.25 (lH,br.s) ,6.19 (lH,br.ε) ,7.36 (5K,ε) . IRfNujol.cπT 1 ) : 3295, 1781, 1684, 1646, 1532. The title compound(58B) waε obtained after deprotection aε described in example 43, followed by salt formation. Yield:57% -H NMR(DMSO-d 6 ,δ) :0.93 (6H,d,J=6.6) ,2.00-2.16 (IH,m) , 2.64-2.69 (IH, m) , 2.79(lH,dd,J=3.7,16.6) , 3.29-3.50 (4H,m) , 3.60 (IH, d,J=4.8) , 3.77- 3.88(lH,m) ,4.27-4.42 (lH.m) ,5.34 (lH,ε) ,8.16 (2H,br.ε) ,8.68-8.81 (lH,m) . IR(Nujol,cm-l) :3350,3225,1716, 1670. Example 55 (3RS, 5RS) -3-N- (D-2-N-Benzyloxycarbonylamino-3-methylbutyryl) aminomethvl-4-oxa-l-azabicvclo[3.2.01heptan-7-one (59A) and (3RS.5RS) -3-N- (D-2-Amino-3-methylbutyryl) aminomethyl-4-oxa-l- azabicvclof3.2.01 eotan-7-one hvdrochlorid (59B. According to the same procedure described in example 43 , N- protected title compound(59A) was obtained as εolid. Yield:55% m.p. :141-143°C 1 H NMR(CDCl3,δ) : 0.91-0.99 (6H,m) , 2.15 (IK, q, =6.4) , 2.59-2.72 (lH,m) , 2.82(lH,d,J=16.3) , 3.28 (IK, a,J=16.3 ) , 3.36-2.62 (2K,m) , 3.39-4.00 (2H,m) ,4.28-4.41 (lH,m) ,5.12 (2H,s) ,5.25 (IK, d,J=2.6) ,5.31 (lH,br.s) , 6.20(lH,br.ε) ,7.36(5H,s) . The title compound(59B) was obtained after deprotection aε described in example 43, followed by εalt formation. Yield:31% λ E NMR(DMSO-d 6 ,δ) :0.93 (6H,a,J=6.6) ,2.00-2.16 (lH.m) , 2.64-2.69 (IH, ) ,2.79(lH,dd,J=3.7,16.6) , 3.29-3.50 (4H,m) , 3.60 (IH,d,J=4.8) , .77- 3.88(lH,m) ,4.27-4.42 (lH,m) ,5.34 (IK, ε) , 8. IS (2H,br.ε) ,8.68-8.81 (lH,m) . IR(Nujol,cm-l) : 3350, 3225, 1716,1670. Example 60 (3RS.5SR) -3- (D-2-Benzvloxvcarbonviamino- " -methylbutvrvl) - oxvmethvl-4-oxa-l-azabicvciof3.2.01hen a -^-one(60A) and (3RS.5SR)-3-(D-2-Amino-3-methvlb tvryl)o-χ methvl-4-oxa-l- azabicvclo \3.2.01 eotan-7-one hvdrochloride(60B) According to the same procedure described in example 43 , N- protected title compound(60A) waε obtained. Yield:85% H NMR(CDCl3.δ) :0.91(3H,a,J=6.9) , 0.99 (3H, a,J=6.9) , 2.10-2.22 (IH, m) ,2.79-2.89 (2H,m) ,3.27 (IH,d,J=16.1) , 3.93-4.33 (3H,m) ,4.51-4.56 (lH,m) ,5.11(2H,s) ,5.24(lK,d,J=9.0) , 5.32 (IK,d,J=2.6) ,7.35(5H,s) . The title compound(60B) was obtained after deprotection as described in example 43, followed by salt formation. Yield:70% X H NMR(DMSO-d 6 ,δ) : 0.88-0.96 (6K,ir.) , 2.09-2.15 (lH,m) , 2.68-2.96 (2H, m) ,3.27-3.40 (IK,m) ,3.77-4.24 (4K.m) ,4.30-4.53 (IK,m) ,5.28 (IK, a, J=2.6) ,8.46(3H,br.ε) . IR(Nujol,cm-l) :3255, 1781, 748,1635. Example 1 (3RS, 5SR) -3- (D-2-Acetylamino-3-methylbutyryl) oxymethyl-4-oxa-l- azabicvclo [3.2.01heptan-" 7 -one (6 1 According to the same procedure described in example 44, title compound(61) was obtained. Yield:49% NMR(DMSO-ds,δ) : 0.90 (6K,m) , 2.05 (3H, ε) , 2.10-3.15 (lH,m) , 2.75-2.90 (2H,m) ,3.30(lH,d,J=16.2) ,2.90-4.15 (6K,m) , .35 (IK, a,J=2.6) ,5.92 (lH,d,J=2.5) . IRtNujol.cm "1 ) :3315, 2945, 1794, 1743, 1665. Example 62 (3RS .5SR) -3- [D-2-N- (D-2-N-Benzvloxvcarbcnvlamino-3-methvlbutvrvl) amino-3-methvlbutvrvl1oxvmethvl-4-oxa-l-azabicvclof3.2.01heptan- 7-one(62A) and (3RS .5 SR) -3- f D-2 -N- (D-2- Am.ino-3 -methvlbutvrvl) amino-3-methvlbut-vrvl1oxvmethvl-4-oxa-l-azabicvclof3.2.01heptan- 7-one hγ3rpch prifle(6?B) According to the same procedure described in example 41, N- protected title compound(62A) waε obtained. Yield:70% E NMR(CDCl3,δ) : 0.92-0.99 (12H,m) , 2.09-2.22 (2H,m) ,2.74-2.89 (2H,m) , 3.30(1H,J=16.2) ,3.94-4.24(4H,m) , 4.52-4.5 (2H,m) , 5.12 (2H, s) , 5.33 (2H,m) ,6.36(lH,d,J=8.5) ,7.34(5K,ε) . The title compound(62B) waε obtained after deprotection aε deεcribed in example 43, followed by salt formation. Yield: 58%. X H NMR(DMSO-dg,δ) : 0.96 (12H, d,J=c .7) ,2.06-:.16(2H.m),2.71-2.82 (2H,m) ,3.38-3.39 (2H,m) ,3. " 6-3.59 (2H,m) ,4.17-4.23 (3H,m) ,4.51-4.5: (lH,m) , 5.34(lH,d,J=2.8) , 5.23 (3K,br.ε) , S . 3 (lH,d, =7.1) . IRWujol, - 1 ) : 3355, 3215, 1784, 1748, 1681, 1554. Example 63 (3RS.5RS) -3-N- (L-2-N-Benzyloxycarbonylam r.c-3-phenylpropanoyl) (3RS.5RS)-3-N-(L-2-Amino-3-phenvlpropanovl)aminomethvl-4-oxa-l- azabicvclo[3.2.01heptan-7-one hvdrochlorid (63B) According to the same procedure described in example 43 , N- protected title compound(63A) waε obtained aε white εoli . Yield:55% m.p. :149-151°C -E NMR(CDCl3,δ) :2.44 (IH,da,J=7.2 , 11.7) , 2.56 (IH, da,J=7.2, 11.9) , 2.76(lH,d,J=16.7) , 2.96-2.27 (4H,m) , 3.77-3.90 (lH,m) , 4.10-4.30 (IH, m) ,4.31-4.43 (lH,m) , 5.04 (IK,a,J=2.6) ,5.09(2K,ε) , 5.18 (IH, d,J=2.6) , 5.28 (IH,br.s) ,5.98 (IH,br.s) ,7.27-7.35 (5H,m) . IR(Nujol,cm _1 ) : 3315, 1783, 1681, 1665. The title compound(63B) was obtained after deprotection as described in example 43, followed by salt formation. m.p. :220-233°C(dec. ) E NMR(DMSO-d 6 ,δ) :2.45 (IH,dd,J=6.7 , 11.8) ,2.62 (IH,dd,J=6.7, 11.6) , 2.76(lH,d,J= 16.3) , 3.06-3.39 (10K,m) , 3.59-3.78 (2H,m) , .04 (lH,br . t, J=6.7) ,4.17-4.18 (lH,m) , 5.22 (IK, a, J=2.3 ) , 5.28 (IH, a, =2.4) ,7.22- 7.36(10H,m) , 8.37 (4H,br . ε) , 8.74 (2K,m) . πUNujol.crrr 1 ) .3205,1776,1561. Example ^ (3RS.5RS) -3-N- f -2-N- ( -2-N-Benzvioxycarbonviamino-3-carboxy propanoyl) amino-3-phenvipropanoyl1 aminomethyl-4-oxa-l-azabicvclc- f3.2.01heptan-7-one (64A) and (3Rg .5RS) -3-N- fL-2-N- ( -2-Amino-3- carboxypropanoyl ) amino-3-phenylpropanoyll aminomethyl-4-oxa-l- azabicvclo[3.2.0Ine p tan-7-one(6451 A mixture of compound63B, obtained in example 63 (500mσ) , 3-N- Benzyloxycarbonylaminc-3-benzyloxycarbonyipropanoic acid(822mg) , 1,3-Dicyclohexyl-carbidiimide (475mg) , Triethylamine(0.35ml) in Dichloromethane (20ml) waε εtirred at room temperature for 2hrε. Resulted mixture was loaded on silicagel column, eluted with Hexane-Ethyl acetate(1:2-1: 3) and protected dipeptide(64A) waε obtained aε εolid. Yield:51% -H NMR(CDCl3,δ) :2. 4-2.61 (lH,m) , 2.76 (IH,a, =16.4) , 2.90-3.12 (3H, m) ,3.20(lH,dd,J=2.7,16.3) , 3.33 (2K, t,J=5.3) , 3.77-3.88 (lH,m) , ' 4.19- 4.23 (lH.m) , 4.52-4.61 (lH,m) , 5.08 (2H,ε) , 5.10 (2H,ε) , 5.20 (IH,d,J= 2.6) , 5.71 (IH,br.s) , 6.27 (lκ,br.ε) , 6.79 (lH,br.d,J=7.7) ,7.16-7.36 (10H,m) . IR(Nujol.cm-l) : 3285, 1782, 1730, 1693. The protected compound, obtained above, was deprotected as in example 46 and the title compound(64B) was obtained as solid. ! H NMR(DMSO-d 6 ,δ) :1.70-1.96 (IH, ) , 2.19-2.36 (lH,m) ,2.67 (IH,dd,J= 6.4,11.5) ,2.78(lH,d,J=17.1) , 3.04-3.45 (3H,m) , 3.12 (IH,dd,J=2.9, 16.5) ,3.78(lH,dd,J=6.5,11.5) , 4.23-4.47 (lK,m) , 5.31 (IH,a,J=2.6) , 6.72 (2H,br.s) , 8.35 (lH,br.s) . Example 65 (3RS.5SR) -3- (L-3-N-Benzvioxycarbonylaminc-3-benzyloxycarbonyl propanovl)oxvmethvl-4-oxa-l-azabicvcio f3.2.01heptan-7-one(65A) and (3RS . SR) -3- (L-3-Amino-3-carboχypropanoyl) oxyτnethyl-4-oxa-l- azabicvclo[3.2. Olheotan-7-one( 5=^ A mixture of (3RS, 5SR) -3-Ξromomethyi-4-oxa-l-azabicyclo [3.2.0] heptan-7-one (1. Og) , (3-N-Ξenzyloxycarbonyianimo-3-benzyloxy- carbonyDpropanoic acid(2.5g), Cesium carbonate (1.63g) and Hexamethylphosphoric triamide(10ml) was heated with εtirring at 60-70°C for 16hrs. The reaction mixture was then diluted with Ethyl acetate, washed with water, brine and dried over Magnesium sulfate. The residual oil after removal cf solvent in vacuo, was purified by silicagel column chromatography eluted with Hexane- Ethyl acetate (1:1). The desired title compound(65A) waε obtained as oil. Yield: 86%. -E NMR(CDCl3,δ) :2.62-3.26 (5K,m) , 3.83-4.20 (3H,m) ,4.36-4.46 (lH,m) , 4.66-4.75 (lH,m) , 5.10 (2H, ε} , 5.16 (2K, ε) , 5.23-5.26 (lH,m) , 5.74-5.84 (lH,m) ,7.30(10H,ε) . IRWujol.cnT 1 ) :3355,2965, 1787,1745. The protected ester, obtained above, was deprotected as described in example 46 and the title compound(65B) was obtained as solid. Yield: 69%. m.p. :175°C. *H NMR(DMSO-d 6 ,δ) :2.57-3.00 (4H,m) ,3.23-3.70 (2H,m) , 3.83-3.98 (lH,m) , 4 . 13-4 . 25 (2H, m) , 4 . 53 -6 . 67 ( lH, m) , 5 . 39 ( IH, d , J=2 . 5 ) . IR Nujol . crrr 1 ) : 3130 , 1793 , 1736 . Example 66 (3RS.5SR) -3- (L-2-N-Benzvloxvcarbonvlamino-3-benzvloxvcarbonvl propanovl)oxvmethv1 -4-oxa-l-azabicvclo [3.2.01heptan-7-on (66A) and (3RS .5SR) -3- ( -2-Amino-3-carboxypropanoyl) oxv ethvl-4-oxa-l- azabicvcloT3.2. Olheotan-7-one(66B) According to the same procedure described in example 65, protected title compound(66A) was cbtained. Yield:74%. 2 H NMR(CDCl3,δ) :2.68-2.86 (lH,m) , 2.79 (IH, dd,J=2.4 , 6.5) ,2.90-3.10 (lH,m) ,3.19(lH,d,J=16.3) ,2.84-3.96(lH,m) ,4.17-4.22(2H,m) ,4.41- 4.56 (lH,m) ,4.62-4.77 (lH,m) ,5.12(4H,s) , 5.26 (IH, a,J=2.6) ,5.74(1H, br.d) ,7.34(5H,ε) ,7.36(5H,s) . IR(Neat, cm "1 ) :3355, 1781, 1753 , 1731, 1518. The protected compound, obtained above, was deprotected aε deεcribed in example 46 and the title compound(66B) waε obtained aε εolid. Yield:55% m.p. :150-180°C(dec. ) . H NMR(DMSO-d 6 ,δ) :2.77-2.64 (IK,m) , 2.91 (IK, dd,J=4.3 , 11.2) ,2.94 (IK, d,J=16.7) ,3.40(lH,dd,J=1.6,19.6) , 4.05 (IK, d,J=7.0, 11.9) ,4.28-4.52 (3H,m) ,4.70-4.79 (lH,m) , 5.46 (IH,a,J=2.4) . IR(Nujol, cm "1 ) :3375, 1776, 1752. Example 67 (3RS.5RS) -3-N- (L-2-N-Benzvioxvcarbonvlamino-3-benzvloxvcarbonvl propanovl)aminomethvl-4-oxa-l-azabicvclo r 2.2.01heptan-7-one(67A) and ( RS.5RS) -3-N- ( -2-Amino-3-carboxypropanoyl)aminomethvl-4- oxa-l-azabicvclor3.2.01heptan-7-σne(67B) (3RS, 5RS) -3-Azidomethyl-4-oxa-l-azabicyclc[3.2.0]heptan-7-one, (l.Og), waε hydrogenated by using 10% Palladium on activated carbon(250mg) at 50psi for lhr. After removal of catalyst, L-(2- N-Benzyloxycarbonylamino-3-benzyioxycabonyl)propanoic acid (2.55g), and Triethylamine (0.1ml) was added to the filtrate, followed by dropwise addition cf a solution of 1, 3-Dicyclohexyl- carbodiimide (1.47g) in Ethyl acetate(10ml) under ice-cooled condition. After lhr stirring, the reaction mixture was loaded on εilicagel column and eluted with Hexane-Ethyl acetate(2 :3) . The deεired protected amide derivative(67A) waε obtained aε oil. Yield:50% 1 H NMR(CDCl3,δ) :2.56-2.71 (IK,m) , 2.61 (IK, , =16.7) , 3.06-3.18 (lH,m) ,3.26(lH,dd, ) , 2.36-3.52 (2K,m) , 3.63-3.94 (lH,m) , 4.23-4.37 (lH.m) ,4.50-4.64 (lK,m) , 5.13 (4K, a,J=2.8) , 5.24-5.29 (lH,m) , 5.90(lH,br,d) , 6.71 (lH,br, s) ,7.34 (5H,s) ,7.36(5H,ε) . IR(Neat, cm "1 ) :3330, 1778, 1727 , 1667 , 1529. The protected amido derivative obtained above waε deprotected aε deεcribed in example 46 and the title compound(67B) was obtained as solid. Yield:74% m.p.275-285°C(dec. ) X H NMR(DMSO-θ 6 ,δ) :2.65-2.86 (3H,m) , 2.93 (IH, a,J=17.2) ,4.40(lH,dd, J=2.2,16.7) ,3.47-3.55(2H,m) , 4.00 (IH,da,J=6.6 , 11.9) ,4.23 (lH,dd, J=5.6,7.4) ,4.51-4.56(lH,m) ,5.43 (lK,d,J=2.6) . IR(Nujol,cm _1 ) :3215, 1677, 1562. Example 68 (3RS.5SR) -3- (D-4-N-Benzvioxvcarbonvlamino-4-benzvloxvcarbonvl butvrvl)oxvmethvl-4-oxa-l-azabicvclof3.2. Olhentan-7-one (68A) and (3RS.5SR)-3-(D-4-Amino-4-carboxvbutvrvl)oxvmethvl-4-oxa-l- azabiςyς.Lpr3.2.Q1heptan-7-Qne(68g) According to the same procedure as example 65, protected title compound(68A) was obtained as oil. Yield:48% 1 H NMR(CDCl3,δ) :1.97-2.19 (lH,m) ,2.17-2.30 (iH,m) ,2.40-2.50 (2H,m) , 2.73(lH,dd,J=6.1,11.6) , 3.25 (IH,a,J=16.2) , 3.94 (IH, dd,J=6.9, 11, 6) , 4.05-4.20 (2H,m) ,4.40-4.55 (2H,m) , 5.10 (2H,m) , 5.17 (2H,m) , 5.30 (IH, t, J=2.8) ,5.39 (br.s) ,7.35(10K,s) . IR(Neat,cm -1 ) :3355, 1784, 1739 , 1525. The protected eεter derivative obtained above waε deprotected as described in example 46 and the title compound(68B) was obtained as solid. Yield: 82% m.p. :>250°C(dec. ) λ E NMR(DMSO-d 6 ,δ) : 1.90-2.0 (2H, ) , 2.75 (IH, dd,J=5.9 , 16.4) ,2.50-2.57 (2H,m) ,2.80(lH,d,J=16.4) ,2.33 (IK,dd,J=2.6, 16.2) , 3.84 (IH,dd,J=7.1, 11.6) ,4.12(2H,d,J=3.4) ,4.52-4.60 (IH,m) , 5.32 (IH, a,J=2.5) . Example 69 (3RS.5RS) -3-N- (D-4-N-Benzvioxycarbonylamino-4-benzyloxycarbonyl butvrvl)aminomethvl-4-oxa-l-azabicvclof3.2.01heptan-7-one(69A) and (3RS.5RS)-3-N-(D-4-Am.ino-4-carboxybutyryl)aminomethyl-4-oxa- l-azabicvclof3.2.01heptan--7-one(69B) According to the same procedure as example 67, protected title compound(69A) waε obtained aε εolid. Yield:22% NMR(CDCl3,δ) :1.90-2.10 (lH,m) ,2.11-2.35 (3H,m) , 2.60-2.72 (lH,m) , 2.81(lH,d,J=16.2) ,3.20-3.60(3H,m) , 3.92 (lH,dd,J=7.4, 12.0) ,4.30- 4.50 (lH,m) ,5.11 (2H,ε) ,5.17 (2H,ε) ,5.30 (lH,s) ,5.59 (lH,br.ε) ,6.16 (lH,br.s) ,7.35(10H,s) . IR(Neat,cm "1 ) :3345, 1783, 1720, 1654, 1533. The protected amido derivative obtained above was deprotected as described in example 46 and the title compound(69B) was obtained as solid. Yield:48% !H NMR(DMSO-d 6 ,δ) :1.74-1.83 (IH,m) , 1.84-1.93 (IH,m) , 2.26 (2H, t,J= 7.9) ,2.67(lH,dd,J=6.4,11.5) , 2.77 (IH,d,J=16.3) , 3.13-3.18 (lH,m) , 3.19-3.22 (lH,m) ,3.25-T .33(lH,m) , 3.32 (IH,dd,J=2.7 , 16.3) ,3.77(1H, dd,J=6.6, 11.6) , 4.28-^.34 (lH,m) , 5.31 (lH,d,J=2.9) , 7, 30 (lH,br.ε) , 8.33(lH.br,ε) . Example 70 (3RS, 5SR) -3- (L-2-N-Benzvipχvcarbonylammc-3-hvdroxypropanoyl) oxymethyl-4-oxa-l-azabicvclo [3.2.01heptan- ~ -one (70) 780mg (3.8 mmol) of (3RS, 5SR) -3-Bromomethyi-4-oxa-l-azabicyclo [3.2.0]heptan-7-one and l.Og of sodium salt of - (2-N-Benzyloxy- carbonylamino-3-hydroxy)propanoic acid in 0.8 ml of Hexamethyl¬ phosphoric triamide was heated at 65°C fcr 3hrs. General work-up and purification gave the title compound aε oil. Yield: 64%. λ E NMR(CDCl3.δ) :2.19-2.32 (lH,m) , 2.75-2.87 (2H,m) ,3.21-3.32 (IH,dd, J=2.6,13.6) ,3.92-4.55(7H,m) , 5.13 (2H, ε) , 5.32 (IH, ε) , 5.66-5.74 (IH, m) ,7.34(5H,ε) . IR(Nujol,cm -1 ) :3430,2963, 1780, 1751. Example 71 (3RS.5SR)-3-[L-2-N-(D-2-N-3enzvloxvcarbonvlaminopropanovl)amino- 3-hvdroxvpropanovlloxvmethvi-4-oxa-l-azabicvciof3.2.Olheotan-7- one(71) 600mg (1.65mmol) of compound(70) and 450mg of 10% Palladium on activated carbon in 20 ml Ethyl acetate waε shaken under Hydrogen atmosphere for lhr and filtered through celite. To the filtrate 370mg (l.δmmol) of D-2- (N-Benzyloxy-carbonyl)aminopropanoic acid and 170mg (O.δOmmol) of 1, 3-Dicyclohexylcarbodiimide were added under cooling and stirred at room temperature fcr 3 hrε. The εolid was removed by filtration and the solution was concentrated. The residue was purified by flash column using Ethyl acetate- Hexane(4:l) as eluent. The title compound(71) was obtained as solid. Yield:3%. m.p. :150-155°C. !H NMR(CDCl3,δ) : 1.41 (3H,d,_T=7. C) , 2.38 (IK, s) , 2.77-2.90 (2H,m) , 3.27 - 62 - (lH,dd, =2.3,11.5) ,3.85-4.57(6K,m) ,5.12 (2H,s) ,5.34 (lH,s: 95 (lH,d,J=7.2) , 7.35(5H,s) , .56 (1H, J=7.6) . Example 72 (3R5.5SR) -3-L- (2-N-Benzvioxycarbonylamino-3-benzvioxypropanoyl) oxvmethvl-4-oxa-l-azabicvclCi3. 2 .0Thentar-7-one (72 ) According to the same procedure aε example 61, the title compound (72) was obtained as oil. Yield: 94%. X H NMR(CDCl3,δ) :2.70-2.82 (2H,m) , 2.14-3.22 (lH,m) , 2.66-3.74 (lH,m) , 3.84-3.95(2H,m) ,4.16-4.33 (2H,rr.) , 4.44-4.56 (4H,m) , 5. 2 2H,ε) ,5.24 (lH,dd,J=2.3,6.7) ,5.62(lK,d,J=4.5) , 7.25-7.36 (10K, m) . IR(Neat, cm -1 ) :3345, 1781, 1718. Example 7 3 ( 3RS . 5SR) -3 - r -2-N- (D-2-N-Benzvloxvcarbonvlaminopropanovl ) amino- 3 -benzvioxvpropanovl 1 oxvmethvl-4 -oxa-l-azabicvclo [ .2 . 01 heptan-7- gne(73) According to the same procedure as example 41, the title compound (73) was obtained as solid. Yield:34%. m.p. :107-108°C. E NMR(CDCl3,δ) : 1. 8 (3H, a,J=2.6) , 2.68-2.80 (2H,m) , 3.14-3.22 (lH.m) , 3.60-3.70(lH,m) , 3.85-3.94 (2H,ιr.) , 4.04-4.55 (6H,m) , 4.70-4.78 (IH,m) , 5.10(2H,s) ,5.25 (lH,dd,J=2.3, 6.8) ,5.30-5.38 (lH,m) ,6.85-6.92 (lH,m) ,7.25-7.34(10H,m) . IR(Nujol,cm _1 ) :3315,1780. Example 74 (3RS, 5SR) -3- [L-2-N- (L-2-N-Benzyloxycarbonylamino-3-methylbutyryl) amino-3-henzvlo χyp ro p anovioxvmethyll -4-cxa- " -azabicvclo T3.2.01 heptan-7-one (74A) and (2RS.5SR -3- ι L-2-N- (L-2- -Amino-3- methylbutyryl ) amino-3-benzyloxypropanovicxymethyll -4-oxa-l- azabicvr-lof3.2.01heptaπ-7-o ne hvdrochloride (74E^ According to the εame procedure aε example 41, protected title compound(74A) was obtained as solid. Yield:51%. m.p. :100-102°C. ! H NMR(CDCl3,δ) : 0.90-0.95 (3H,a,J=3.0) , 0.95-1.04 (3H,d,J=2.8) , 2.10- 2.18(lH,m) ,2.67-2.82 (2H,m) , 3.17-3.27 (lH,m) ,3.62-3.68(lH,m) ,3.86- 3.96(2H,m) ,4.05-4.26 (3H,m) ,4.42-4.56 (3H,m) , 4.72-4.78 (lH,m) ,5.13 (2H,ε) ,5.23 (lH,dd,J=2.4,16.2) , 5.34 (IH, a,J=4.5) , 6.58 (IH, d,J=4.4) , 7.25-7.36(10H,m) . IRtNujol.c -1 ) :3320, 2970, 1781. The title compound(74B) was obtained after deprotection as ' described in example 43, followed by salt formation. yield:77%. m.p.:168°C (dec) -H NMR(DMSO-de,δ) : 0.98-1.18 (6H,m) ,2.12-2.22 (lK,m) , 2.72-2.86 (2H,m) , 3.28-3.34(lH,m) , 3.68-3.94 (4H,m) , 4.20-4.28 (2H,m) ,4.52-4.77 (4H,m) , 5.34(lH,dd,J=2.5,16.2) ,7.40 (5H, ε) , 8.24-8.32 (3H, ε) , 9.06 (IH,d, J=8.1) . IRfNujol.cnr 1 ) -.3335,1772,1678. Example 75 (3RS.5SR)-3-(L-2-Acetamiάo-3-hvάroxvpropanovl)oxvmethvl-4-oxa-l- azabicvclor3.2.01heptan-7-one(75A) and (3RS .55R) -3- (2-Acetamido- 3-ac oxvpropanovl) oxvmethvl-4-oxa-1-azabicvcic r 2.2.01heotan-7- one(75B) 500mg (1.3 mmol) of the compound 72 waε deprotected aε usual manner. To this 140mg (1.37mmol. cf Trie rylamine was added and cooled to 0° . llOmg (1.37mmol) Acetyl chloride waε added, εtirred at the same temperature fcr lhr fcliowed by at room temperature for lhr. The solution was filtered and the filtrate was concentrated. The compound was purified over silica gel using Ethyl acetate first and then Ethyl acetate-Acetone (5:1) . Two fractions were collected. N-Acetyl compound(75A) and N,0-diacetyl compound(75B) were obtained. Monoacetyl compound (75A) : Yield:39% λ E NMR(CDCl3,δ) : 2.07 (6H, ε) , 2.75-2.90 (2H,m) , 3.25-3.38 (lH,m) , 3.95- 4.07 (lH,m) ,4.24-4.36(3H,m) , 4.50-4.60 (2H,m) , 4.84-4.92 (lH,m) , 5.34 (lH,d,J=2.5) , 6.20-6.30 (lH,m) . IRtNujol.cm -1 ) :3335,2960,1779,1664. Diacetyl compound(75B) : Yield:26% *H NMR(CDCl3,δ) : 2.08 (3H, s) , 2.44 (IK, t,J=6.9) , 2.77-2.90 (2H,m)', 3.30 (lH,dd,J=2.8,12.2) , 3.95-4.40 (5H,m) , 4.54-4.64 (lH,m) ,4.70-4.78 (lH,m) , 5.36(lH,d,J=2.6) , 6.38-6.46 (lH,m) . IR(Neat, cm "1 ) :3360, 2968, 1774, 1652. Example 76 (3RS.5RS)-3-fN- (L-2-N-Benzvloxvcarbonvlammo-3-hvdroxvpropanovl1 aminomethvll -4-oxa-l-azabicvclo r3.2.01heptan-7-one (76A) and (3RS.5RS) -3- fN- ( -2-Amino-3-hvάrcxvpropanovl) aminomethvll -4-oxa- l-azabicvclo r3.2.01heptan-7-one hvdrochloride (76B) According to the same procedure described in example 43 , N- protected title compound(76A) was obtained aε white εolid. Yield:38% m.p. : 114-116, 124.5-137°C X H NMR(CDCl3,δ) :2.69 (IH, da,J=6.2 , 11.0, ) , 2.51 (IH, ,J=15.8) ,3.25 (lH,d,J=16.3) ,3.47(2H, ,J=5.3) ,3.62-3.77 ( K,m) ,3.85-3.47 (3H,m) , 4.17-4.30 (lH,m) ,4.30-4.43 (lH,m) ,5.13 (2H,s) ,5.28-5.31 (lH,m) , 6.11- 6.21(lH,m) ,7.10 (IH,br.s) ,7.36 (5H,s) . IR(Nujol,cm -1 ) : 3280, 1772, 1553, 1554, 1457. The title compound(76B) was obtained after deprotection as described in example 43, followed by εalt formation. Yield:38% X H NMR(D 2 0,δ) : 2.72-2.88 (IK, ) , 2. 1 (lH,a, =13.3 ) , 3.39 (IH,dd,J=2.6, 16.3) ,3.50(2H,d,J=5.3) , 3.7:- .20 (4H,m) ,4.462H,m) ,5.46(lH,d, J=2.6) . IRtNujol.cm "1 ) : 3360, 3325,1766, 1679, 1630. Example 77 (3RS.5SR) -3- (L-2 , 6-N-Diber_zyloxycarbonylaminohexanoyl)oxymethyl- 4-oxa-l-azabicvclo[3.2.0Tneptan-7-one(77A) and (3RS.5SR) -3- (L- 2.6-Diacetamidohexanovl)oxvmethvl-4-oxa-l-azabicvclof3.2.01- heotan-7-one(77B) According to the same procedure described in example 40, (2,6- Dibenzyloxycarbonylamino)hexanoyloxy derivative (77A) was obtained aε thick oil. Yield:76% H NMR(CDCl3,δ) : 1. 2-1.80 (6H,m) , 2.73-2.86 (2H,m) ,3.18-3.30 (3H,m) , 3.91-4.52 (5H,m) , 4386-5340 (7H,m) ,7.33(10H,s) . IR(Neat, cirri) :3355, 3070, 2960, 1781, 1751, 1721. To a εolution of (2, 6-Dibenzyloxycarbonylamino)hexanoyloxy derivative(77A) obtained above (1.8g, 3.33mmol) and Acetic anhydride (3.2ml,3.33mmol) in Ethyl acetate (100ml) waε added Palladium on activated caroon (10%, 53.8% moist,2.0 g) and the mixture was hydrogenolysea at 50 psi for 2 hr. The catalyst was filtered off over a pad cf celite and the solution was concentrated. Purification on a silica gel column uεing Ethyl acetate-acetone (1:2:5) as the eluent gave a thick oil(77B) . Yield:63%. 1 H NMR(CDCl3,δ) : 1.19-1.87 (6K,m) ,1.98(3K,ε , 2.04 (3K, s) , 2.76-2.89 (2H,m) ,3.14-3.34(3H,m) , 3. 3 (IH, d ,J=ll .6, " .0) , 4.10-4.25 (2H,m) , 4.47-4.58 (2H,m) ,5.36 (lH,br.s) ,5.89 (lH,br.z) ,6.49 (lH,d,J=7.0) . IR(Neat,cm -1 ) :3320, 3075,2693 , 1784, 1762 , 1741, 1656. Example 7B (3RS.5SR)-3-(L-5-acetamiάe-2-N-Benzvloxvcarbonylaminohexanovl) oxvmethvl-4-oxa-l-azabicvclof3.2.01heptar.- 7 -one(78) To a Ethyl acetate(60ml) εolution of 2, 5-diber.zyloxycarbonylaminc hexanoyloxy derivative (77A) , (1.Og, 1.85mr.ol) waε added Palladium on activated carbon (10%,1.0g) and the mixture waε hydrogenolyεed at 50 psi for 2 h. The mixture was filtered through a pad of celite and cooled in an ice-bath. Acetic anhydride (0. 4ml,4. δδmmol) and Triethylamine (0.77ml) were added and the mixture was stirred for 2 h and washed with water (60ml) and brine. The Ethyl acetate solution was dried over Magnesium sulfate, concentrated and passed through a silica gel column chromatography uεing Ethyl acetate aε the eluent to give an oil(78) Yield:12%. X H NMR(CDCl3,δ) :1.29-1.89 (6H,m) ,2.01 (3H, ε) ,2.72-2.88 (2H,m) ,3.14- 3.31(3H,m) ,3.96 (IH,dd,J=ll.6, 6.9) , 4.13-4.29 (2H,m) , 4.53-4.56 (2H, m) ,4.97 (lH,br.t) ,5.09 (2H,ε) ,5.32 (lH,br.ε) ,6.29 (lK,d,J=7.0) ,7.34 (5H ,ε) . IRWujol,™- 1 ) : 3335, 2950, 1783, 1740, 1717, 1659. Example " 9 (3RS.5SR)-3- (L-2-N-Benzvlcxvcarbonvlaminc-3-p ' nenvl)propanovl- oxymethyl-4-oxa-l-azabicvclo [3.2. 1hepta - " -one (79) According to the εame procedure described in example 43, N- protected title compound (79) was obtained. Yield: 63% X H NMR(CDCl3,δ) : 2.57-2.74 (IH,m) , 2.82 (IK, a,J=16.2) , 3.11 (2H, a, J=6.1) ,3.25(lH,dd,J=2.3,16.0) , 2.53-3.95 (lK,m) , 4.12-4.23 (2H,m) , 4.40-4.55 (lH,m) , 4.59-4.75 (lH,m) ,5.10(2H,ε) , 5.12-5.27 (2H,m) ,7.20- 7.38(10H,m) . IR(Nea , cm-1) :3335, 1784 , 1747 , 1722. ■ΕST EXAMPLE Tr. Vitrc B Cei: Cvtotox c tv Assay In vitro KB cell cytoxocity asεay was done by modification of the crystal violet assay (Grillis et al.. Anal Biochem. , 15 , 109-113 (1986) . KB cells were cultivated m Eagles minimum essential medium supplemented with 10% calf serum and incubated at 37°C in a humidified 5% CO2 atmosphere to prepare a cell stock. Cells were counted uεing a neubauer hemocytometer and seeded in 96 well plates at 100 μl of 3 x 10 4 cells/ml and cultured for one day. Teεt compounds were diluted and 100 μl of the solution was added in triplicate wells to give final concentration of 10, 5, 1, 0.5, 0.1, 0.05 and O.Olμg/ml. Control wells were identical except that test compound was absent. These were cultured fcr three days. Then the cells were fixed with addition cf 20 μl of 25% glutaraldehyde for 15 minutes, washed with water and dried ' . Then stained with 100 μl of 0.05% crystal violet for ISminutes, washed with water and dried. The wells are eluted with lOOμl of 0.05M NaH2PC> /ethanol (1:1 v/v) and read at OD 540 on a multiscan spectrophotometer. Inhibition value of cell growth was calculated based on optical density uεing the following equation; untreated - treated % inhibition = X 100 untreated TD5 0 valueε were calculated from linear regression ines of the log- logit plot. The compound of formula (I) waε aεεayed by thiε method againεt KB cell lineε and their TD5Q values are reported in Table 1. p ^m τ^ XAMPLΞ 2 In Vitro L1210 and P355 Cell Cvtotoxicitv Assay In vitro :L1210 and P388 ceil cytotoxicity assay was done by the method of microculture tetrazoliu asεay (Alley et al. , Cancer Research, 4£. 589-601 (1988) . L1210 and P388 cellε were cultivated in RPMI 1640 medium εupplemented with 10% fetal calf εerum and 50 μl of 2- mercaptoethanol at 37 = C humidified 5% CO2 atmoεphere to prepare a cell stock. Cells were counted using neubauer hemocytometer and seed in 96 well plates at 100 μl of 0.5 x 10 4 cells per ml. The test compounds were diluted and 100 μl of the solution was added in triplicate wells to give the final concentration of 10, 5, 1, 0.5, 0.1, 0.05 and 0.01 μg/ml. Control wells were identical except that the test compound waε abεent. These were cultured for three days. Reεultε were aεεayed using the microculture tetrazolium assay briefly. 50 μl of MTT formazoan working solution (1:5 v/v in culture medium) was added to each well and cultures were incubated at 37°C for 4 hrε. Culture plateε were centrifuged at low speed for 5 minuteε . All but 10-20 μl of culture medium supernatant was removed by slow aspiration and replaced by mechanical shaker and read at OD540 on a multiscan spectrophotometer. Inhibition value of cell growth waε calculated based on optical denεity uεing the following equation; untreated - treated % inhibition = X 100 untreated TD 50 valueε were calculated from linear regression ines of the log- logit plot. The compound of formula (I) was assayed by this method against L1210 and P388 cell lines and their TD50 values are reported in Table 2. Table 1 (continued) Table 1 (continued) KB Cell Cytotoxi _:ity (TD50 μσ/ml) (D)COCH[CH(CH3) 2]NHCOCH3 0.385 (D)COCH[CH(CH 3 )2_NK- 0.3B1 (D) -COCH[CH(CH3)2] H -HCl (L)COCH(CH 2 C 6 E 5 )NH 2 .KC1 0.573 (L)COCH(CH 2 C6H5)NH- 2.65 (L) -COCH(NH2)CH2COOH (L)COCH 2 CH(NH2)COQE C.451 (L)COCH<NH )CH COOH 0.204 (L)CCCH(NH2)CK2COOH 6.04 (D)CO!CH2)2 H( H2)COOH 0.189 (D)CO(CH2) 2~ H (NH2)COOK 2.47 (L)COCH(CH 2 OH)NHZ 0.352 (L)COCH(CH2θH)NH- 0.549 (D)-COCH(CH3)NHZ (L)COCH(CH2OCH2C6H5)KHZ 1.67 ( )COCH(CH2θCE2C6H5) H- 2.22 (D)-COCH(CH 3 )NHZ 74B 0 (L)COCH(CK2OCH2C6H5)NH- 0.669 (L)-COCH[CH(CH )2JNH2 •HCl (L)COCH(CH2OH)NHCOCH3 1.9B (L)COCH(CH2OCOCH3)NHCOCH3 0.567 (L)CCCH(CE2OH)NH 2 .HCl 8.81 (L)COCH(NKZ) (CH 2 >4NHZ 1.18 _I_ ) 0CH ( NHC0CH2 ) I H^ 2^ z 3.38 Z;Benzyloxycarbonyl TABLE 2 Tn vi ro Cell Toxicitv of Compounds; cf General Formula I Aσainst Sensitive and Resistant Tumor Cell Lines Cell Toxicity(μg/ml) Example No. L-1210(Ξ) L-1210 (R) ?388(S) P388(R) 1 0.103 0.104 3.103 0.074 G0069A 2.17 1.67 1.02 0.751 Adriamycin 0.004 0.35 C.003 0.575 Vincristine 0.002 0.20 2.95xicr 7 0.279 TEST EXAMPLE ~ In Vivo Antitumor Activity Against Sarcoma 180 The compounds of general formula (I) were tested in vivo against Sarcoma 180 xenografted tumor to mice as illustrated herein after. Sarcoma 180, 5xl0 6 cells were inoculated by S.C. to male ICR mice (6 weeks old) on day 0. Drugs were administered on days 1, 5 and 9. Mice were killed and tumor weight was measured on day 12 after transplantation. The percentage inhibition of tumor growth was calculated from the mean tumor weight cf the treated group compared with that of the control group. Number of mice used in each group was between 6 to 7. The percentage inhibition of tumor Sarcoma 180 group by compound cf formula (I) are summarized in Table 3. TABLE 3 Effect of Compounds of Formula (I) Against Sarcoma 180 (s.c. - i.p. ) in Male ICR Example No. Dose Mor ality % Inhibition 4 100 0/6 49.4 6 80 40 20 Effect of Compounds Against Sarcoma 180 (contonued) Example No. Dose Mortality % Inhibition (mg/kg/dav' > in 12 davs 2/6 39.8 Effect cf Compounds Against Sarcoma 18C (contonued) Example No, Dose Mortality % Inhibition (mσ/kσ/dav in 12 davs Effect cf Compound;. Aσair.st Sarcoma 16C (contonued) Example No. Dose Mortality % Inhibition (mg/ko/dav' in 12 davs 0/6 77.7 0/6 49.8 0/6 42.1 0/6 55.0 0/6 65.9 0/6 41.1 3/6 80.3 0/6 69.7 0/6 49.1 0/6 37.8

Claims: GLAIMS 1. 3-(substituted methyl)-4-oxa-l-azabicyclo[3.2.0]heptan- 7-one derivatives of formula I or a pharmaceutically acceptable salt thereof, wherein X is NH or 0, when X is NH, R is hydrogen, -CORi, wherein Ri is (i) a ι-C alkyl group which may be substituted by 1-3 substituents selected from halogen, hydroxy, formyloxy, azido, carboxyl, heteroaryl or acetamide, (ii) a-C 2 -C alkenyl group, (iii) a C 2 -C 4 alkynyl group, (iv) a C3-C6 cycloalkyl group, (v) a phenyl group which may be substituted by 1-3 substituents selected from hydroxy, halogen, Ci-Cβ alkoxy group or cyano, (vi) a C5-C6 heteroaryl group, (vii) a NR 2 R 3 wherein R 2 and R 3 are the same or different and each is a hydrogen, Ci-Cβ alkyl group or (7-oxo-l-aza-4-oxabicyclo[3.2.0]hept-3- yl)methyl group or (viii) benzyloxy group; -SO 2 R 4 wherein R 4 is a C 1 -C 6 alkyl group or a phenyl group which may be substituted by 1-3 substituents selected from Ci-Cβ alkyl group, halogen or C 1 -C6 alkoxy group; 1-2 amino acid residue which may be substituted with protective group, when X is 0, R is 1-2 amino acid residue which may be substituted with protective group. 2. The derivatives of formula I as set forth in claim 1 wherein X is NH and R is -CORi wherein Ri is ι~Ce alkyl group which may be substituted by 1-3 substituents selected from halogen, hydroxy, formyloxy, a∑ido, carboxy, heteroaryl or acetamido. 3. The derivatives of formula I as set forth in claim 1 wherein X is NH and R is -CORi wherein Ri is a NR 2 R 3 wherein R 2 and R 3 are the same or different and each is a hydrogen, C 1 -C 6 alkyl group or (7-oxo-i-aza-4-oxabicyclo[3.2.0]hept-3- yl)methyl group. 4. The derivatives of formula I as set forth in claim 1 wherein X is NH or 0 and R is 1-2 amino acid residue which may be substituted with protective group. 5. The derivatives cf formula I as set forth in claim 1 wherein X is NH, having (3R,5R) or (3S,5S) configuration at two asymmetric carbons on 4-oxa-l-azabicyclo[3.2.0]heptan-7- one ring system or the mixture of them. 6. The derivatives cf formula I as set forth in claim 1 wherein X is 0, having (3R,5S) or (3S,5R) configuration at two asymmetric carbons on 4-oxa-l-azabiyclo[3.2.0] eptan-7- one ring system or the mixture of them. 7. The pharmaceutically acceptable salts of formula I as set forth in claim 1 are selected from sodium, potassium, magnesium, calcium, hydrogen chloride, tartaric acid, succinic acid, fumaric acid or p-toluenesulfonic acid. 8. A pharmaceutical composition comprising of a compound as set forth in claim 1 and pharmaceutically acceptable carrier. 9. An antitumor composition comprising of an effective amount of a compound as set forth in claim 1 and pharmaceutically acceptable carrier. A process for preparing 3- (substituted methyl)-4-oxa-l- azabicyclo[3.2.0]heptan-7-one derivatives having the general formula I when X is 0; The said process comprising the reaction of compound having general formula II with substituted carboxylic acid in presence of base in solvent, when X is NH; The compound having general formula II was converted to amino by reaction with metal azide followed by reduction in presence of metal catalyst and the resulting amino compound was reacted with acid halide, acid anhydride or activated ester in presence of base in solvent, wherein R and X are same as defined as above and L is an appropriate leaving group.

Inventors: FIAKPUI CHARLES, HA CHAN, MATSUMOTO HIROSHI, MICETICH RONALD, OIE SHINJI, OTANI TOSHIO, SINGH RAJESHWAR, THOMAS GEORGE, YAMASHITA TOMOHIRO, FIAKPUI, CHARLES, HA, CHAN, MATSUMOTO, HIROSHI, MICETICH, RONALD, OIE, SHINJI, OTANI, TOSHIO, SINGH, RAJESHWAR, THOMAS, GEORGE, YAMASHITA, TOMOHIRO
Applicants: APPLEDENE LIMITED, FIAKPUI CHARLES, HA CHAN, MATSUMOTO HIROSHI, MICETICH RONALD, OIE SHINJI, OTANI TOSHIO, SINGH RAJESHWAR, SYNPHAR LAB INC, TAIHO PHARMACEUTICAL CO LIMITE, THOMAS GEORGE, YAMASHITA TOMOHIRO, FIAKPUI, CHARLES, HA, CHAN, MATSUMOTO, HIROSHI, MICETICH, RONALD, OIE, SHINJI, OTANI, TOSHIO, SINGH, RAJESHWAR, SYNPHAR LABORATORIES, INC., TAIHO PHARMACEUTICAL CO. LIMITED, THOMAS, GEORGE, YAMASHITA, TOMOHIRO
Application year: 1995
Main classification: C07D503
IPC codes: A61K31, A61P35, C07D503