OLIGONUCLEOTIDE N3'=>P5' PHOSPHORAMIDATES: TRIPLEX DNA FORMATION

Abstract: Modified oligonucleotides 3'-NHP(O)(O<->)O-5' phosphoramidates were synthesized on a solid phase support. The phosphoramidate analogs were found to have significantly increased resistance toward phosphodiesterase digestion. Thermal dissociation experiments demonstrated that these compounds form more stable duplexes than phosphodiesters with complementary DNA and particularly RNA strands. Further, the phosphoramidate analogs can also form stable triplexes with double-stranded DNA target, where under similar conditions parent phosphodiester compounds failed to do so.

Description: OLIGONUCLEOTIDE N3 * →P5- * PHOSPHORAMIDATES: SYNTHESIS AND COMPOUNDS: HYBRIDIZATION AND NUCLEASE RESISTANCE PROPERTIES FIELD OF THE INVENTION The present invention relates to the compounds and compositions of N3'-»P5' phosphoramidates, synthesis methods and hybridization and nuclease resistance methods employing oligonucleotide N3'→P5' phosphoramidates. BACKGROUND OF THE INVENTION Oligonucleotides have been proposed as potent diagnostic compounds and as new rationally designed therapeutic agents (Uhlman, 1990; Helene, et al, 1990; Helene, 1991). The mechanism of action of these compounds is based on their specific interaction with RNA or DNA regions of interest. Several modifications of the natural phosphodiester internucleoside bond {phosphomono- (Eckstein, et al., 1985; Cohen, 1993) or dithioate (Marshall, et al., 1993), methylphosphonate (Miller, 1991), phosphodiester amidate (Letsinger, et al., 1988; Froehler, et al., 1988)} have been introduced to improve (i) the stability of the oligomers in biological media, and (ii) the hybridization properties of the oligomers. Unfortunately, " the vast majority of these analogs exhibit reduced binding with target RNA or DNA strands via duplex or triplex formation (Kibler-Herzog, et al., 1991). Moreover, the presence of the stereoisomers at phosphorous in some of these analogs may complicate the binding patterns with complimentary nucleic acids (LaPlauche, et al., 1986; Bower, et al., 1987; Tidd, et al., 1988). SUMMARY OF THE INVENTION Methods, compounds and compositions of the present invention relate to oligodeoxyribonucleotides having contiguous nucleoside subunits joined by intersubunit linkages. In the oligonucleotides, at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'-*P5' phosphoramidate intersubunit linkages. An exemplary N3'-*P5' phosphoramidate intersubunit linkage is: Where X is -O , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl. An exemplary N3'-»P5' phosphoramidate oligonucleotide is: Formula 1 Where X is -O", -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, n is from 4 to 100 and B is a base. Normally the 5' oxygen is either bonded to a hydrogen or another nucleotide in the oligonucleotide and the 3' oxygen is bonded to another nucleotide in the oligonucleotide. For definitions of these exemplary substituent groups see the Definitions section below. The nucleoside subunits making up the oligodeoxyribonucleotides of the present invention can be selected to be in a defined sequence: such as, a sequence of bases complementary to a single-strand nucleic acid target sequence or a sequence that will allow formation of a triplex between the oligodeoxyribonucleotide and a target duplex. In one embodiment the oligodeoxyribonucleotide has at least 3 contiguous subunits joined by N3'→P5' phosphoramidate linkages. This grouping of linkages can, for example, be located at the 3' end of the oligodeoxyribonucleotide. At this location the N3'→P5' phosphoramidate linkages confer nuclease resistance to the oligodeoxyribonucleotide. In another embodiment of the present invention, all of the intersubunit linkages are N3'→P5' phosphoramidate linkages. The present invention includes intermediates used to make the oligodeoxyribonucleotides and methods for synthesizing such intermediates. The intermediates are of the following formulas: and The present invention includes a method for generating a duplex nucleic acid molecule by forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where the oligodeoxyribonucleotide contains intersubunit linkages of Formula 1 or such linkages in combination with other linkages discussed herein. The oligodeoxyribonucleotide has a sequence of nucleoside subunits to form a duplex with a target nucleic acid molecule when the oligodeoxyribonucleotide is contacted with the target nucleic acid molecule under conditions to allow effective formation of a duplex. The present invention also includes a method for blocking an interaction of a protein with a polynucleotide by forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages of Formula 1 or such linkages in combination with other linkages discussed herein. The oligodeoxyribonucleotide has a sequence of nucleoside subunits to form a duplex or a triplex with a target nucleic acid molecule when the oligonucleotide contacted with the target nucleic acid under conditions to allow effective formation of a duplex or triplex. This method can be used inside a cell. Also included in the invention are oligodeoxyribonucleotides where the intersubunit linkages alternate the N3'→P5' phosphoramidate linkage and a second linkage. The second linkage may be selected from one or more different types of linkages, for example, phosphodiester linkages or phosphodiester and phosphorothioate linkages. The second linkage is selected, for example, from the group consisting of phosphodiester, phosphotriester, methylphosphonate, phosphoramidate P3'→N5', and phosphorothioate. In one embodiment at least 50% of the intersubunit linkages are N3'->P5' phosphoramidate linkages. The present invention includes a method for generating a triplex DNA molecule, by forming an oligodeoxyribonucleotide as described above, where the oligodeoxyribonucleotide has a sequence of nucleoside subunits effective to form triple-helix structure with a target duplex DNA. The oligodeoxyribonucleotide is then contacted with the duplex DNA under conditions effective to allow formation of a triplex between the oligodeoxyribonucleotide and the duplex target DNA. This method can be carried out under a variety of conditions, for example, intracellularly or in solution. The present invention also includes a triplex DNA molecule, having three DNA strands: (i) a duplex DNA molecule, having two complementary strands, and (ii) bound to the duplex a third strand oligodeoxyribonucleotide having N3'→P5' phosphoramidate linkages as discussed above. In one embodiment, 50% or greater of the intersubunit linkages of the third strand oligodeoxyribonucleotide are N3'→P5' phosphoramidate linkages, including fully modified oligodeoxyribonucleotides. Further, the invention includes a method of enhancing the resistance of an oligodeoxyribonucleotide to nuclease digestion. In this method an oligodeoxyribonucleotide is formed having N3'-*P5' phosphoramidate linkages as described above. The oligodeoxyribonucleotide is exposed to nuclease. Such oligodeoxyribonucleotides are more resistant to nuclease digestion than a corresponding oligodeoxyribonucleotide having only phosphodiester intersubunit linkages. Nuclease resistance is observed intracellularly as well. The oligodeoxyribonucleotides having N3'→P5' phosphoramidate linkages, as described above, have superior hybridization properties. The present invention also includes a method of enhancing hybridization of a first oligodeoxyribonucleotide to an RNA target sequence, where the oligodeoxyribonucleotide has contiguous nucleoside subunits joined by N3'→P5' phosphoramidate intersubunit linkages. In the method, a second oligodeoxyribonucleotide having N3'→P5' phosphoramidate linkages is formed having the same sequence of contiguous nucleoside subunits as the first oligodeoxyribonucleotide. The second oligodeoxyribonucleotide is effective to hybridize to said target RNA sequence. The second oligodeoxyribonucleotide is then contacted with the RNA under conditions effective to allow formation of a complex between the oligodeoxyribonucleotide and the RNA. Such contacting can be carried out under a variety of conditions, including intracellularly. The present invention also includes a method and kit for the isolation of a target RNA from a sample. The kit includes an oligodeoxyribonucleotide having N3'→P5' phosphoramidate linkages, as described above, where the oligodeoxyribonucleotide is effective to hybridize to the target RNA sequence. Typically, the oligodeoxyribonucleotide is attached to a solid support, such as a magnetic bead, to facilitate isolation. In another embodiment, the present invention includes a diagnostic method to detect the presence in a sample of an RNA having a selected target sequence. In this method, oligodeoxyribonucleotides having N3'→P5' phosphoramidate linkages are created that are effective to form a hybridization complex with a target sequence. The oligodeoxyribonucleotide is then contacted with the sample under conditions effective to allow formation of the hybridization complex between the oligodeoxyribonucleotide and the target sequence. The presence of the hybridization complex is then detected. Detection of the hybridization complex can be accomplished by labelling the oligodeoxyribonucleotide with a reporter moiety, where detecting includes detection of the reporter moiety. Numerous reporter moieties are available, including, but not limited to, radioactive labels, biotin labels, and fluorescent labels. This detection method can be carried out under a variety of conditions including intracellularly. Similar diagnostic methods can be carried out using oligodeoxyribonucleotide having N3'→P5' phosphoramidate linkages, where the target sequences are duplex DNA or single- stranded DNA. In the case of detection of duplex DNA, detection of the hybridization complex can be accomplished using a gel band shift assay. For detection of single-stranded DNA the oligodeoxyribonucleotide typically contains greater than 50% of the total intersubunit linkages as N3'→P5' phosphoramidate intersubunit linkages. The present invention also includes a duplex oligodeoxyribonucleotide, having (i) two complementary strands, and (ii) contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit linkages, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages (as described above). In one embodiment, 50% or greater of the intersubunit linkages of at least one strand are N3'→P5' phosphoramidate linkages. In another embodiment all of the intersubunit linkages of at least one strand are N3'-*P5' phosphoramidate linkages. Such duplex DNA molecules may also include a flexible hinge region connecting the complementary strands. The hinge region may connect the strands in any desired polarity, e.g., 5' to 3', 3' to 5', 3' to 3', and 5' to 5'. Further, the present invention includes a method of forming a triplex nucleic acid complex, having two complementary DNA strands and one RNA strand containing a target region, and compositions thereof. In the method, an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages is formed. The oligodeoxyribonucleotide is capable of forming a duplex oligodeoxyribonucleotide, having (i) two complementary strands with 5' and 3' ends, (ii) contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'-*P5' phosphoramidate intersubunit linkages (as described above), (iii) where the strands are connected from the end of one strand to the end of the other strand by a flexible hinge region, and (iv) the complementary oligodeoxyribonucleotide strands having a sequence of nucleoside subunits effective to form triple-helix structure with the RNA target. The oligodeoxyribonucleotide is then contacted with the RNA target under conditions effective to allow formation of a triplex between the oligodeoxyribonucleotide and the RNA. This method can be carried out under a variety of conditions, including, intracellularly. The present invention also includes pharmaceutical compositions of oligodeoxyribonucleotides having N3'→P5' phosphoramidate linkages, as described above. The oligodeoxyribonucleotides are useful in therapeutic applications based on hybridization, such as, antigene and antisense applications. These and other objects and features of the invention will be more fully appreciated when the following detailed description of the invention is read in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE FIGURES Figures 1A to ID show the structures of subunits useful for the synthesis of oligonucleotides having internucleoside 3'-NHP(O)(O " )O-5' phosphoramidate linkages (N3'→P5'). Figure IE shows a schematic outline of the step-by-step synthesis of uniformly modified oligonucleotides. In the figure, CE = cyanoethyl and CPG = controlled pore glass. Figures 2A to 2E present exemplary combinations of 3'-NHP(O)(O')O-5' phosphoramidate intersubunit linkages with other, alternative linkages. In Figures 2D and 2F, R' is, for example, a lower alkyl group, other substitutions are possible as described by Goodchild (1990). Figure 3 presents exemplary oligonucleotides and T m values of duplexes and triplexes. In the figure: a = The T m 's of complexes in the buffer A. b = T m in the buffer; T m of the hairpin duplex was 55.7 and 61.5°C in buffer A and B, respectively. c = Mismatched nucleotide is underlined. Figures 4 A to 4C. Figure 4A presents an IE HPLC profile of the reaction mixture after synthesis of the phosphoramidate Oligonucleotide 3. Figure 4B presents a capillary gel electrophoresis profile of the reaction mixture after synthesis of the undecaphosphoramidate 6. Figure 4C shows the results of 31 P-NMR of the decaphosphoramidate 3. Figures 5A to 5B display melting curves for the duplexes, formed by phosphodiester and phosphoramidate oligomers. Figure 6 shows exemplary oligonucleotide hairpins and their T m values. Figures 7 A to 7D show melting curves for the triplexes. Figure 8 shows gel-electrophoresis analysis of the oligonucleotide triplex formation under native conditions. Figure 9 shows gel-electrophoresis analysis of the oligonucleotide triplex formation under native conditions. Figures 10 through 19 show the results of anti-sense oligonucleotides, having either phosphoramidate (N3'→P5') (Figures 10-15) or phosphorothioate (Figures 16-19) intersubunit linkages, on leukemia cell proliferation for different BCR-ABL leukemia cell lines and control cell lines. Figure 20 schematically represents the preparation of 3'-amino-N 6 -benzoyl-5'- dimethoxytrityl-2',3'-dideoxyadenosine. REFERENCES Abergo, D.D., et al., Biochemistry 20:1409-1413 (1981). Agrawal, S., et al., Proc. Natl. Acad. Sci. 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Seelig, R., et al, Leukemia 7(11): 1886-1887 (1993). Shabarova, Z.A., Biochemie 70:1323-1334 (1988). Stein, CA. and Cheng, Y.-C, Science 261:1004-1012 (1993). Szczylik, C, et al, Science 253:562-565 (1991). Tidd, D.M., et al, Anti-Cancer Drug Design 3:117-127 (1988). Uhlman, E., et al, Chem. Rev. 90:544-584 (1990). Vickers, T., et al, Nuc. Acids Res. 19(12):3359-3368 (1991). Weeks, K.M., et al, Science 249:1281-1285 (1990). Wickstrom, E., Editor, Prospects for Antisense Nucleic Acid Therapy of Cancer and AIDS. Wiley-Liss, New York, NY (1991). Wilson, W.D., et al, Biochemistry 32(40): 10614-10621 (1993). Zalewski, A., et al, Circulation Res. 88: 1190-1195 (1993). Zamecnik, P.C., et al, Proc. Natl. Acad. Sci. USA 83:4143-4146 (1986). Zielinski, W.S., et al, Nucl. Acids Res. 15: 1699-1715 (1987). DETAILED DESCRIPTION OF THE INVENTION I. DEFINITIONS An "alkyl group" refers to an alkyl or substituted alkyl group having 1 to 20 carbon atoms, such as methyl, ethyl, propyl, and the like. Lower alkyl typically refers to C, to C 5 . Intermediate alkyl typically refers to C 6 to C 10 . Similarly, "cycloalkyl group" refers to a saturated carbocyclic ring group which may have alkyl, aryl, aralkyl substituents such as cyclopropyl, cyclopentyl, cyclohexyl, and the like, or a substituted form thereof. An "alkenyl group" refers to a hydrocarbon group containing a carbon-carbon double bond, such as vinyl, allyl, cyclopentenyl, and the like. An "alkenyl group" also refers to substituted alkenyls. Lower alkenyl typically refers to C, to C 5 . Intermediate alkenyl typically refers to C 6 to C ι0 . An "aryl group" refers to an aromatic ring group having 5-20 carbon atoms, such as phenyl, naphthyl, anthryl, or substituted aryl groups, such as, alkyl- or aryl-substitutions like tolyl, ethylphenyl, biphenylyl, etc. Also included are heterocyclic aromatic ring groups having one or more nitrogen, oxygen, or sulfur atoms in the ring. An "aralkyl group" refers to substituted alkyl group, such as, aryl-substitutions like benzyl, phenethyl, naphthyl etc. and alkyl is defined as above. By "substituted" it is generally meant that the group is derivatized with one or more small chemical moieties, e.g. , methoxy, ethoxy, halogen, hydroxyl, cyano, amido, amine and ethylene oxide. Any of the groups defined above may be substituted, for example, (- CF 3 ). "Oligonucleotides" typically refer to nucleoside subunit polymers having between about 4 and about 50 contiguous subunits. The nucleoside subunits can be joined by a variety of intersubunit linkages, including, but not limited to, those shown in Figures 2A to 2E. Further, "oligonucleotides" includes modifications, known to one skilled in the art, to the sugar backbone (e.g., ribose or deoxyribose subunits), the sugar (e.g., substitutions), the base, and the 3' and 5' termini. "Oligodeoxyribonucleotides" include such modifications, such as, 2' sugar substitutions of fluorine. In Formula 1 n typically is 4 to 100 or 5-100, preferably 6 to 60, more preferably 6 to 40, and most preferably 6 to 30. Generally the total oligonucleotide length will vary from 6 to 100 nucleotides, preferably 6 to 50 nucleotides, and more preferably 10 to 30 nucleotides. In the oligonucleotides, generally at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunits, preferably at least 3 contiguous subunits, more preferably at least 4 contiguous subunits, and most preferably at least 5 contiguous units. Generally oligonucleotides of the invention will not have mismatches to the target sequence. If the oligonucleotide is longer than about 20 to 30 nucleotides mismatches of 1 or 2 or 3 bases are permitted. "Nucleoside" is defined herein as a pentose sugar (a ribose, deoxyribose, or modification thereof) bound to a base capable forming hydrogen bonds (typically a purine or pyrimidine). A "base" is defined herein to include (i) typical DNA and RNA bases (uracil, thymine, adenine, guanine, and cytosine), and (ii) modified bases or base analogs (e.g., 5- methyl-cytosine, 5-bromouracil, or inosine). A base analog is a chemical whose molecular structure mimics that of a typical DNA or RNA base. A "triplex" has three nucleic and acid strands. Generally oligonucleotides of the invention will not have mismatches to the target sequence. If the oligonucleotide is longer than about 20 to 30 nucleotides mismatches of 1 or 2 or 3 bases are permitted. II. THE PRESENT INVENTION Experiments performed in support of the present invention demonstrate that oligonucleotides containing achiral internucleoside 3'-NHP(O)(O " )O-5' phosphoramidate linkages (N3'-»P5') are more resistant to nuclease digestion and have improved RNA and dsDNA hybridization characteristics relative to oligonucleotides not containing N3'- * P5' phosphoramidate linkages. Oligonucleotides containing the N3'→P5' linkages have excellent antisense activity against complementary mRNA targets in in vitro cell growth inhibition assays. Further, the oligonucleotides exhibit low cytotoxicity. A. SYNTHESIS AND CHARACTERIZATION OF OLIGONUCLEOTIDES CONTAINING INTERNUCLEOSIDE S ' -NHProXO O-S' PHOSPHORAMIDATE LINKAGES Oligonucleotides, containing single N3'→P5' phosphoramidate linkage were prepared by chemical ligation in aqueous media (essentially as described by Shabarova, 1988). Alternatively, oligonucleotides containing N3'→P5' linkages between two subunits where the next intersubunit bond was at least one phosphodiester bond, were synthesized on a solid support via coupling of the preformed phosphoramidate dimer blocks (Gryaznov, et al, 1992; Mag, et al., 1992). Random size ribooligonucleotide N3'→P5' phosphoramidates were obtained via self-polymerization of dimer blocks (Zielinski, et al, 1987). Azhayer, et al, describe the synthesis of defined sequence oligoribonucleotides. The present invention includes solid support synthesis methods for the generation of oligodeoxyribonucleotides with contiguous nucleoside subunits joined by N3'→P5' phosphoramidate intersubunit linkages (np) (Example 1, Figure 1). Sequential synthesis of oligodeoxyribonucleotides utilizes 5'-dimethoxytrityl-3'-amino-deoxyribonucleotide subunits. The preparation of each of these subunits is described in Example 1 (see, for example, Figure 20) and outlined as follows: Oligonucleotides having contiguous subunits joined by N3'→P5' phosphoramidate intersubunit linkages (e.g. , uniformly modified) were synthesized on a solid support using the step-by-step elongation procedure outlined in Example 1 and as shown below: The synthetic cycle for addition of a single aminonucleoside consists essentially of the following operations: detritylation (Figure 1, step i); phosphitylation of the 5'- hydroxyl group to generate a polymer supported 5'-H-phosphonate diester (Figure 1 , steps ii, iii); Atherton-Todd type (Atherton, et al, 1945; Gryaznov, et al, 1992; Gryaznov, et al, 1986; Gryaznov, et al, 1990) coupling of a 5'-dimethoxytrityl-3'-amino nucleoside (Glinski, et al, 1970) with the 5'-H -phosphorate in the presence of carbon tetrachloride (Figure 1, step iv). This cycle can be repeated several times resulting in phosphoramidate oligonucleotide after deprotection with ammonia (Figure 1, step v, vi). Average coupling yields were 94-96% per step as judged by dimethoxytrityl (DMT) cation assay. Exemplary oligodeoxyribonucleotides containing N3'→P5' phosphoramidate linkages ("np") are presented in Figure 3 and throughout the specification. Oligodeoxyribonucleotides of the present invention contain at least two contiguous subunits joined by N3'-*P5' phosphoramidate intersubunit linkages (for example, 5'-T-np-G- np-A-3'), or greater than 3 total N3'→P5' phosphoramidate intersubunit linkages. In one embodiment, the oligodeoxyribonucleotides contain fully modified N3'→P5' phosphoramidate intersubunit linkages (e.g., Figure 3, experiment 13, oligonucleotide 6). In another embodiment, the oligodeoxyribonucleotides have alternating N3'→P5' phosphoramidate intersubunit linkages between subunits, typically alternating with phosphodiester or phosphorothioate linkages (see exemplary linkages below and in Figure 2). A example of such an oligodeoxyribonucleotide having alternating linkages is shown in Figure 3, experiment 3, oligonucleotide 2. Synthesis of oligonucleotide 2 is described in Example 1. Oligonucleotides were isolated by ion exchange high performance liquid chromatography (IE HPLC; Example 2, Figure 4A). Purities of isolated oligonucleotide preparations were evaluated by capillary electrophoresis and slab gel electrophoresis analysis (Example 2, Figure 4B). Presence of the phosphoramidate linkages in the purified oligonucleotides was confirmed by 31 P-NMR (Example 2, Figure 4C) and by selective acid-catalyzed hydrolysis of phosphoramidate linkages (Example 2). The cyanoester group in Figure 1 (step i, iii) can be replaced by other pendent groups, including, alkyl (usually lower or intermediate alkyls), alkenyl, aryl and aralkyl groups (or substitutions of any of the preceding groups). Typically, such pendant groups do not interfere with the synthesis of oligonucleotides or the ability of the oligonucleotide to hybridize to a target. One exemplary pendent group is -CH 3 (Gryaznov, et al., 1992). A typical repeat unit is shown in Figure 2A, where "X" is "-O ", "-OR" or "-R", and "R" is, for example, any of the following pendent groups or substitutions thereof: alkyl, alkenyl, aryl, and aralkyl. In addition to phosphoramidate analogs and chimeric phosphoramidate/phosphodiester analogs (Figure 2C), the internucleoside N3'→P5' phosphoramidate linkages can be incorporated into oligonucleotides having one or more other modified intersubunit linkages (reviewed by Goodchild, 1990), including, but not limited to, phosphotriesters (Figure 2D), methylphosphonates (Figure 2B), phosphoramidates (Figure 2F), phosphoramidates P3'-»N5', and phosphorothioates (Figure 2E). B. NUCLEASE RESISTANCE OF OLIGONUCLEOTIDES CONTAINING ACHIRAL INTERNUCLEOSIDE 3'-NHP(TJ)(O O-5 * PHOSPHORAMIDATE LINKAGES Stability of the oligonucleotide phosphoramidates toward hydrolysis by snake venom phosphodiesterase was evaluated in comparison with natural phosphodiester compounds (see Materials and Methods). Phosphodiester decamer Oligonucleotide 1 (Figure 3) was treated with snake venom phosphodiesterase. The oligonucleotide 1 was completely hydrolyzed after 10 minutes, as judged by reversed phase high performance liquid chromatography (HPLC). In contrast, phosphoramidate analog Oligonucleotide 3 was essentially intact even after 50 minutes of treatment with snake venom phosphodiesterase. After 4.5 hours, approximately 50% of Oligonucleotide 3 was converted to the presumed 9-mer (TnpT) 4 T NH2 with a terminal 3'-amino group. The presence of the terminal 3'-amino group retarded further digestion of the oligomer. After 22 hours of hydrolysis, the starting 10-mer Oligonucleotide 3 was completely converted to the 3'-amino-terminal 9-mer {(Tnp) 8 T NH2 }. Only about 20% further digestion of the {(Tnp) 8 T NH2 } compound was observed. These results demonstrate the increased nuclease resistance of oligonucleotides containing N3'→P5' phosphoramidate linkages ("np"), relative to oligonucleotides having standard phosphodiester backbones. In one embodiment of the present invention, nuclease resistance of oligodeoxyribonucleotides is generated by placing approximately 3 contiguous subunits linked by N3'-*P5' phosphoramidate intersubunit linkages at the 3' end of the ol igodeoxyribonucleotides . C. HYBRIDIZATION PROPERTIES OF OLIGONUCLEOTIDES CONTAINING N3 * →P5' PHOSPHORAMIDATE LINKAGES The hybridization properties of the phosphoramidate analogs were evaluated relative to complementary DNA or RNA strands having standard phosphodiester intersubunit linkages. The thermal stability data for duplexes generated from phosphoramidate analogs and phosphodiester oligomers are summarized in Figure 3 (Example 1). Exemplary melting curve data (Example 4A) for duplexes formed by phosphodiester and phosphoramidate analog oligomers are presented in Figures 5 A and 5B. In the figures, curves (A), (C), (B), and (D) correspond to experiments 8, 9, 13 and 14 in Figure 3, respectively. Substitution of the internucleoside phosphodiester for the N3'→P5' phosphoramidate linkages dramatically changed the oligonucleotides' hybridization properties. Melting temperatures (T m 's) of duplexes formed by the entirely modified 10-mer Oligonucleotide 3 with poly Da (i.e., DNA) and poly A (i.e., RNA) were 36.0°C and 51.5°C, respectively (Figure 3, experiments 5 and 6). These Tm's are 6.3 °C and 24.5 °C higher than duplexes formed by the phosphodiester counteφart Oligonucleotide 1 with poly Da and poly A (Figure 3, experiments 1 and 2). The same trend is true for the mixed-base undecanucleotide 6 (Figure 3), where the T„-'s of duplexes with complementary DNA and RNA strands were 49.2°C and 72.4°C, respectively, (Figure 3, experiments 13, 14). These values are 11.7°C and 22.9°C higher than for the parent phosphodiester compound Oligodeoxyribonucleotide 4 (Figure 3, experiments 8 and 9). Also, the duplex with the same RNA target formed by phosphoramidate 11-mer Oligodeoxyribonucleotide 6 is more stable (by 18.0°C) than one formed by the homologous RNA oligomer 5 (Figure 3, experiment 11). Oligodeoxyribonucleotide 2, with alternating phosphodiester - phosphoramidate linkages, also binds more tightly with the RNA strand, T m 33.7°C (Figure 3, experiment 4) than the corresponding phosphodiester compound (Oligodeoxyribonucleotide 1, Figure 3, experiment 2). However, Oligodeoxyribonucleotide 2 binds less strongly with the DNA template, T m 25.8°C, (Figure 3, experiment 3) relative to its phosphodiester counteφart (Oligodeoxyribonucleotide 1, Figure 3, experiment 1). Hybridization of the phosphoramidate oligonucleotides with complementary nucleic acids is sequence specific and determined by the proper Watson-Crick base pairing. The duplex formed by phosphoramidate Oligodeoxyribonucleotide 6 with single mismatch RNA target (Figure 3, experiment 15) is substantially less stable (ΔT m -12.2°C) than the duplex formed with the fully complementary RNA oligomer (Figure 3, experiment 13). About the same mismatch discrimination was observed for the phosphodiester deoxyribo- and ribo- oligonucleotides, where ΔT m was -14.4°C and -12.4°C respectively (Figure 3, experiments 10, 12). A previous study with phosphoramidate analogs demonstrated that introduction of three N3'→P5' phosphoramidate linkages resulted in a destabilization trend, relative to two such linkages, for heteroduplexes formed with deoxyribo-oligonucleotide targets (Gryaznov, et al., 1992). In contrast to the prior art trend, the results presented above demonstrate that having up to 50% of the intersubunit linkages as phosphoramidate linkages typically decreases the stability of DNA/DNA heteroduplexes. Greater than 50% phosphoramidate intersubunit linkages in one strand of a DNA duplex, however, begins to improve stability of the duplex relative. When a DNA duplex is formed between a normal, phosphodiester oligonucleotide and an oligonucleotide fully modified with N3'-*P5' phosphoramidate linkages, the thermal stability of the duplex is much higher than the corresponding duplex having only phosphodiester linkages in both strands (Figure 3, compare experiments 1 and 5). Gryaznov, et al. (1992) only contains data concerning the hybridization properties of DNA/DNA duplexes where one strand contains up to three N3'->P5' phosphoramidate linkages (non-contiguous). In shaφ contrast to the teachings of the prior art concerning DNA targets, experiments performed in support of the present invention demonstrate that having increasing numbers of phosphoramidate analog linkages present in an oligodeoxyribonucleotide increases the stability of DNA/RNA heteroduplexes. To achieve DNA/RNA heteroduplex stabilization, a preferred embodiment of the present invention includes an oligodeoxyribonucleotide having at least 2 contiguous inter subunits, or more than 3 total intersubunit linkages modified to have N3'→P5' phosphoramidate linkages. More preferably oligonucleotides will have at least 5 contiguous intersubunits with N3'→P5' phosphoramidate linkages. Experiments were also performed to evaluate the stability of duplexes formed by oligonucleotides containing phosphoramidate linkages in both complementary strands. Several chimeric phosphoramidate-phosphodiester haiφin oligomers (Figure 6) were synthesized (Figure 6, Example 4B) having thymidine-containing hinge regions (T 4 , Figure 6). Melting curves obtained for these compounds show that the most stable duplexes were formed by the haiφins in Oligonucleotides 9 and 12 — where both strands contain phosphoramidate linkages in opposing positions (Figure 6, experiments 3, 6). Also, duplexes formed from single-strand DNA molecules (i.e., haiφins) where one strand contains alternating phosphoramidate-phosphodiester linkages and the complementary strands has only phosphodiester linkages, are more stable than their solely phosphodiester counteφarts (Figure 6, experiments 1, 4). These results suggest that when both strands of a DNA/DNA duplex contain phosphoramidate analog linkages, the duplex is stabilized by the presence of N3'→P5' phosphoramidate linkages in each strand. Stable duplexes can be formed with one phosphoramidate linkage in each strand — in one embodiment the phosphoramidate linkage is in the same location in each strand. To achieve DNA/DNA duplex stabilization typically 2 or more of the intersubunit linkages in each DNA strand of a duplex forming oligonucleotide are modified to have N3'-»P5' phosphoramidate linkages. In one embodiment, one strand of a haiφin forming DNA oligonucleotide can be modified to have about 50-100% of N3'-*P5' phosphoramidate intersubunit linkages. D. TRIPLEX FORMATION USING PHOSPHORAMIDATE ANALOGS The ability of the phosphoramidate analogs to form triplexes with double-stranded DNA was also evaluated (Example 4). Melting curves were obtained for triplexes formed by the decathymidilic phosphoramidate Oligodeoxyribonucleotide 6 and the dA ι0 :dT 10 duplex region of the haiφin DNA target d(A 10 C 4 T 10 ) (Figure 3, experiment 7). The triplex had a T m of 32°C at close to physiological conditions. A more stable triplex (T m 42.2 C C) was observed in magnesium-containing buffer (Figure 3, experiment 7). The same T m value was obtained for triplexes formed by phosphoramidate Oligonucleotide 3 with poly dA:poly dT duplex. Thermal dissociation of the triplexes was monitored by change of absorbance at 260 nm (Figures 7A and 7C), as well as at 284 nm (Figures 7B and 7D), which is characteristic for T:AT triplexes (Riley, et al, 1966). Results of the gel-shift experiments under native conditions also demonstrate formation of the stable triplex by phosphoramidate decamer 3 and dsdna target (Figure 8), as well as stable triplex formation by Oligonucleotide 6 (Figure 9). Under the same hybridization conditions neither phosphodiester decathymidilic acid Oligonucleotide 1, nor Oligonucleotide 4 formed triplexes with the same double-stranded DNA targets, as judged by the melting curves and by the gel-shift experiments (Figure 8). That is, no corresponding triplex was formed by the oligonucleotides which have phosphodiester intersubunit linkages - suggesting that the phosphoramidate analogs may more readily form triplexes than their phosphodiester containing counteφarts. Similar results were obtained when Oligonucleotide 6 and Oligonucleotide 1 were evaluated for their ability to form triplex structures with duplex DNA targets (Example 5, Figure 9). The results presented above suggest that the phosphoramidate oligonucleotides are more effective for triplex formation, with a duplex substrate, than standard phosphodiester oligonucleotides. III. APPLICATIONS OF OLIGONUCLEOTIDES CONTAINING INTERNUCLEOSIDE 3'- NHPC KO ' IO-S" PHOSPHORAMIDATE LINKAGES Oligonucleotide 3'-NHP(O)(O " )O-5' phosphoramidates were synthesized. These compounds are nuclease resistant and form suφrisingly stable complexes with ssRNA and DNA targets. The N3'-»P5' phosphoramidate analogs have great potential for anti-sense and anti-gene diagnostic/therapeutic applications. In a preferred embodiment of the present invention, the oligonucleotides are oligodeoxyribonucleotides. A. ANTI-SENSE APPLICATIONS Antisense therapy involves the administration of exogenous oligonucleotides that bind to a target nucleic acid, typically an RNA molecule, located within cells. The term antisense is so given because the oligonucleotides are typically complementary to mRNA molecules ("sense strands") which encode a cellular product. The phosphoramidate analog oligonucleotides described herein are useful for antisense inhibition of gene expression (Matsukura et al., 1989; Agrawal et al., 1989; Zamecnik et al., 1986; Rittner and Sczakiel, 1991; Stein and Cheng, 1993). Oligonucleotides containing N3'→P5' phosphoramidate linkages have therapeutic applications for a large number of medically significant targets, including, but not limited to inhibition of cancer cell proliferation and interference with infectious viruses. The N3'→P5' phosphoramidate oligonucleotides are useful for both veterinary and human applications. The low cytotoxicity of these compounds and their ability to act effectively as antisense molecules at low concentrations (see below) make these oligonucleotides highly desirable as therapeutic antisense agents. Anti-sense agents typically need to continuously bind all target RNA molecules so as to inactivate them or alternatively provide a substrate for endogenous ribonuclease H (Rnase H) activity. Sensitivity of RNA/oligonucleotide complexes, generated by the methods of the present invention, to Rnase H digestion can be evaluated by standard methods (Donia, et al, 1993; Kawasaki, et al, 1993). The methods of the present invention provide several advantages over the more conventional anti-sense agents. First, phosphoramidate analog oligonucleotides bind more strongly to RNA targets than corresponding phosphodiester oligonucleotides. Second, the phosphoramidate analog oligonucleotides are more resistant to degradation by cellular nucleases. Further, when an RNA is coded by a mostly purine strand of a duplex target sequence, phosphoramidate analog oligonucleotides targeted to the duplex also have potential for inactivating the DNA — i.e., the ability to inactivate a pathogen in both single- stranded and double-stranded forms (see discussion of anti-gene therapies below). Sequence-specific phosphoramidate analog binding molecules are potentially powerful therapeutics for essentially any disease or condition that in some way involves RNA. Exemplary modes by which such sequences can be targeted for therapeutic applications include: a) targeting RNA sequences expressing products involved in the propagation and/or maintenance of infectious agents, such as, bacteria, viruses, yeast and other fungi, for example, a specific mRNA encoded by an infectious agent; b) formation of a duplex molecule that results in inducing the cleavage of the RNA (e.g., Rnase cleavage of RNA/DNA hybrid duplex molecules); c) blocking the interaction of a protein with an RNA sequence (e.g., the interaction of TAT and TAR, see below); and d) targeting sequences causing inappropriate expression or proliferation of cellular genes: for example, genes associated with cell cycle regulation; inflammatory processes; smooth muscle cell (SMC) proliferation, migration and matrix formation (Liu, et al., 1989); certain genetic disorders; and cancers (protooncogenes). In one embodiment, translation or RNA processing of inappropriately expressed cellular genes is blocked. Exemplary potential target sequences are protooncogenes, for example, including but not limited to the following: c-myc, c-myb, c-fos, c-kit, ras, and BCR/ABL (e.g., Wickstrom; Zalewski, et al, 1993; Calabretta, et al, 1992, 1993;), oncogenes/tumor suppressor genes (e.g., p53, Bayever, et al), transcription factors (e.g. , NFKB, Cogswell, et al, 1993) and viral genes (e.g., papillomaviruses, Cowsert, et al; heφes simplex virus, Kulka, et al). To further illustrate, two RNA regions of the HIV-1 protein that can be targeted by the methods of the present invention are the REV-protein response element (RRE) and the TAT-protein transactivation response element (TAR). REV activity requires the presence of the REV response element (RRE; SEQ ID NO:23), located in the HIV envelope gene (Malim et al., 1989a, 1989b). The RRE has been mapped to a 234-nucleotide region thought to form four stem-loop structures and one branched stem-loop structure (Malim et al., 1989a). Data obtained from footprinting studies (Holland et al., 1990; Kjems et al., 1991) suggest that REV binds to six base pairs in one stem structure and to three nucleotides in an adjacent stem-loop structure of the RRE. A minimum REV binding region of about 40 nucleotides in stem-loop II has been identified by Cook, et al. (1991; SEQ ID NO:24). This binding region can be targeted for generation of RNA/DNA duplexes (e.g., Li, et al, 1993) using one or more oligonucleotides, according to the methods of the present invention. The HIV-1 TAT is essential for viral replication and is a potent transactivator of long terminal repeat (LTR)-directed viral gene expression (Dayton et al., 1986; Fisher et al., 1986). Transactivation induced by TAT protein requires the presence of the TAR element (SEQ ID NO:25) which is located in the untranslated 5' end of the viral mRNA element. The TAR element is capable of forming a stable stem-loop structure (Muesing et al., 1987). The integrity of the stem and a 3 nucleotide (nt) bulge on the stem of TAR has been demonstrated to be essential for specific and high-affinity binding of the TAT protein to the TAR element (Roy et al., 1990; Cordingley et al, 1990; Dingwall et al., 1989; Weeks et al., 1990). This region can be targeted for anti-sense therapy following the method of the present invention. In addition to targeting the RNA binding sites of the REV, RRE and TAT proteins, the RNA coding sequences for the REV and TAT proteins themselves can be targeted in order to block expression of the proteins. The N3'→P5' phosphoramidate oligonucleotides afford unique targeting capabilities to RNA regions that self-anneal to form RNA/RNA hybrids, such as, but not limited to the stem-loop structures of RRE. The N3'→P5' phosphoramidate oligonucleotides displace RNA/RNA hybrids due to high affinity binding of a N3'→P5' phosphoramidate oligonucleotides to one strand of a RNA/RNA hybrid. Binding of a first N3'→P5' phosphoramidate oligonucleotide to a RNA/RNA hybrid causes the formation of a RNA/DNA duplex (one strand RNA and one strand N3'→P5' phosphoramidate oligonucleotide), which prevents the reassociation of the RNA/RNA hybrid and can prevent the binding or recognition of a RNA/RNA hybrid by a protein. Binding of the first N3'→P5' phosphoramidate oligonucleotide could also result in increased cleavage of RNA by Rnases. The greater stability of the RNA/N3'→P5' phosphoramidate duplex, compared to the RNA/RNA hybrid, minimizes the chances of reannealing of the RNA to form a RNA/RNA hybrid. Alternatively, N3'→P5' phosphoramidate oligonucleotide binding to one strand of a RNA/RNA hybrid opens the RNA/RNA hybrid, thereby exposing a second RNA site for a second oligonucleotide to bind. RNA regions that self-anneal to form RNA/RNA hybrids can be identified empirically by demonstrating that N3'→P5' phosphoramidate oligonucleotides displace known RNA binding proteins. RNA regions with RNA/RNA hybrids, such as stem loop structures, can also be identified by computer assisted predications of RNA secondary structure. Programs such as Sixou, S. et al. Nucleic Acid Res. 22:662-668 (1994), the methods are herein incoφorated by reference, are particularly useful for identifying RNA regions that form RNA/RNA hybrids, such as palindromic sequences separated by at least 3 to 7 bases. Initial screening of N3'-»P5' phosphoramidate oligonucleotides, directed to bind potential antisense target sites, typically includes testing for the thermal stability of resultant RNA/DNA duplexes. When a phosphoramidate analog oligonucleotide is identified that binds a selected RNA target sequence, the analog is further tested for inhibition of RNA function in vitro. Cell culture assays systems are used for such in vitro analysis (e.g., heφes simplex virus, Kulka, et al; HIV-1, Li, et al., Vickers, et al.; coronary smooth muscle cell proliferation in restenosis, Zalewski, et al; IL-2R, Grigoriev, et al; c-myb, Baer, et al; c-fos, Cutry, et al; BCR/ABL, Szczylik, et al, 1991). Example 5 presents the results of testing phosphoramidate oligonucleotides in one such cell culture system. The assay measures selective inhibition of leukemia cell proliferation by BCR-ABL antisense oligonucleotides (Szczylik, et al, 1991). BCR-ABL transcripts are found in the majority of chronic myelogenous leukemia (CML) patients and in Ph + acute lymphocytic leukemia patients, and are believed to be necessary for the maintenance of leukemic phenotype (Gale, et al; Collins, et al, 1984; Daley, et al). The BCR-ABL transcripts are the result of a translocation of the proto-oncogene ABL (chromosome 9) to the breakpoint cluster region (BCR) (chromosome 22), resulting in the formation of BCR- ABL hybrid genes. A fully modified N3'→P5' phosphoramidate anti-sense oligonucleotide 11-mer (SEQ ID NO:6), complementary to the identified BCR-ABL junction B2A2 (cell line BV173), was synthesized and purified. A corresponding oligonucleotide 16-mer (SEQ ID NO:26), (i) containing the 11-mer sequence as given above, and (ii) having fully modified phosphorothioate intersubunit linkages, was also prepared. The oligonucleotides were administered to the cells at 24 hour intervals for three days (days 0, 1 and 2) at the concentrations shown in Figures 10 to 15, 16 and 18. Concentrations in the figures are presented as follows: 40/20/20 corresponds to concentrations, in μg/ml, of oligonucleotides as added to the cell cultures (Example 5). Figures 17 and 18 present the results for experiments performed using a 16-mer, having fully modified phosphorothioate intersubunit linkages, with sequence mismatches to the BV173 BCR/ ABL splice junction. The results presented in Figures 10, 12 and 14 demonstrate that the phosphoramidate oligonucleotides were extremely effective at inhibiting BV173 leukemia cell proliferation, regardless of the concentration at which the oligonucleotide was administered. The results presented in Figures 11, 13 and 15 demonstrate that the phosphoramidate oligonucleotides have negligible cytotoxicity. In these experiments, the cell line was either HL60, which contains no BCR/ ABL breakpoint, or K562, which contains the B3A2 BCR/ ABL break point, which is partly non-homologous to the B2A2 BCR ABL break point. On the other hand, when similar experiments were performed using the phosphorothioate 16-mer, the oligonucleotides were not effective at inhibiting BV173 leukemia cell proliferation when administered at comparable concentrations to the phosphoramidate oligonucleotide (Figures 16 and 18). Specifically, leukemia cells appear to begin to be released from inhibition by the phosphorothioate 16-mer at a concentration of about 1.25/0.625/0.625 compared to a similar release of inhibition seen when using the phosphoramidate oligonucleotide at 0.0196/0.0098/0.0098. Accordingly, the results demonstrate that the phosphoramidate oligonucleotides are effective antisense agents at considerably lower concentrations than the widely used phosphorothioate oligonucleotides. Further, the inhibition curves seen with the phosphoramidate oligonucleotide treatment have a downward inflection at later time points, even after the partial release of inhibition (Figure 14). On the other hand, the inhibition curves seen with the phosphorothioate oligonucleotide treatment have a steep upward inflection at later time points, after the partial release of inhibition (Figure 18). These results suggest that the release from inhibition is more dramatic for the phosphorothioate oligonucleotides when compared to the phosphoramidate oligonucleotides. The absence of cytotoxicity with the phosphorothioate 16-mer was comparable to that seen with the phosphoramidate oligonucleotide applied at the same concentration. The results presented above confirm in cell culture the superior qualities of the N3'-*P5' phosphoramidate oligonucleotides demonstrated by the hybridization studies described above. These results support the usefulness and efficacy of the N3'-*P5' phosphoramidate oligonucleotides in antisense and antigene in vivo therapies. Further, the results demonstrate that the N3'→P5' phosphoramidate oligonucleotides provide superior antisense function in vitro than phosphorothioate oligonucleotides. To date, the phosphorothioate backbone modification in oligonucleotides has become the standard for antisense applications, representing the subject analog in more than 95% of the — 2500 antisense journal publications in 1993. In a great variety of assay systems involving a large number of mRNA targets, the antisense phosphorothioate oligonucleotides were required at concentrations of 1-15 μM in order to achieve substantial inhibitory effects. Nevertheless, inhibitory activity at that level was sufficient to commence US FDA approved clinical trials in three diseases targeting three different mRNAs: CML (chronic myelogenous leukemia), IND #42974 and AML (acute myelogenous leukemia) IND #40453 (Antiviral Agents Bulletin, Vol. 5, No. 6, ppl61-162 (1992), ISSN 0897-9871, Biotechnology Information Institute); as well as Hepatitis B Virus (HBV). The data presented in Example 5 demonstrate that the N3'→P5' phosphoramidate oligonucleotides are effective antisense agents at much lower concentrations than corresponding phosphorothioates. Further, the N3'→P5' phosphoramidate oligonucleotides have no apparent cytotoxicity even at the highest concentrations used, which were well above the concentrations required for antisense activity. The results indicate that the N3'→P5' phosphoramidate oligonucleotides are excellent agents for therapeutic applications. In vitro effects of a selected phosphoramidate analog oligonucleotide can be confirmed in an in vivo system. Such in vivo systems include, but are not limited to, the following (target - model system): hepatitis virus - chimpanzee or monkey models; c-myb, c-myc, bcr-abl - SCID mouse models (e.g. , Ratajczak, et al); NF-κB - mouse (Higgins, et al); and pl20 - mouse (Perlakey, et al.). B. ANTI-GENE APPLICATIONS Inhibition of gene expression via triplex formation has been previously demonstrated (Cooney et al., 1989; Orson et al., 1991; Postel et al., 1991). The increased stability of triplex structures formed when employing third strand phosphoramidate analog oligonucleotides provides a stronger tool for anti-gene applications, including veterinary and human therapeutic applications. A target region of choice is selected based on known sequences using standard rules for triplex formation (Helene and Toulme, 1990). Typically, the phosphoramidate analog nucleic acid sequence is targeted against double-stranded genetic sequences in which one strand contains predominantly purines and the other strand contains predominantly pyrimidines. Phosphoramidate analog oligonucleotides of the present invention are tested for triplex formation against a selected duplex target sequences using band shift assays (Example 4). Typically, high percentage polyacrylamide gels are used for band-shift analysis and the levels of denaturing conditions (Ausubel et al.; Sauer et al.; Sambrook et al.) are adjusted to reduce any non-specific background binding. The duplex target is labeled (for example, using a radioactive nucleotide) and mixed with a third strand oligonucleotide, being tested for its ability to form triplex structures with the target duplex. A shift of the mobility of the labelled duplex oligonucleotide indicates the ability of the oligonucleotide to form triplex structures. Triplex formation is indicated in the band shift assay by a decreased mobility in the gel of the labeled triplex structure relative to the labeled duplex structure. Numerous potential target sites can be evaluated by this method including target sites selected from a full range of DNA sequences that vary in length as well as complexity. Se¬ quence-specific phosphoramidate analog binding molecules are potentially powerful therapeutics for essentially any disease or condition that in some way involves DNA. Exemplary target sequences for such therapeutics include: a) DNA sequences involved in the propagation and/or maintenance infectious agents, such as, bacterial, viruses, yeast and other fungi, for example, disrupting the metabolism of an infectious agent; and b) se¬ quences causing inappropriate expression or proliferation of cellular genes, such as oncogenes, for example, blocking or reducing the transcription of inappropriately expressed cellular genes (such as genes associated with certain genetic disorders). Gene expression or replication can be blocked by generating triplex structures in regions to which required regulatory proteins (or molecules) are known to bind (for example, HIV transcription associated factors like promoter initiation sites and SP1 binding sites, McShan, et al). Alternatively, specific sequences within protein-coding regions of genes (e.g., oncogenes) can be targeted as well. When a phosphoramidate analog oligonucleotide is identified that binds a selected duplex target sequence tests, for example, by the gel band shift mobility assay described above, the analog is further tested for its ability to form stable triplex structures in vitro. Cell culture and in vivo assay systems, such as those described above under "Anti-Sense Applications" are used. Target sites can be chosen in the control region of the genes, e.g., in the transcription initiation site or binding regions of regulatory proteins (Helene and Toulme, 1990; Birg et al., 1990; Postel et al., 1991; Cooney et al., 1988). Also, target sites can be chosen such that the target also exists in mRNA sequences (i.e., a transcribed sequence), allowing oligonucleotides directed against the site to function as antisense mediators as well (see above). Also, phosphoramidate modified DNA molecules can be used to generate triplex molecules with a third strand target (i.e., a single-strand nucleic acid). For example, a DNA molecule having two regions capable of forming a triplex structure with a selected target third strand molecule can be synthesized. Typically the two regions are linked by a flexible region which allows the association of the two regions with the third strand to form a triplex. One example of such a DNA molecule is T 10 (fully phosphoramidate modified)- C 4 (hinge region)-T, 0 (phosphodiester linkages). This molecule forms triplex structures with a polyA RNA target. A corresponding DNA molecule having T 10 (phosphodiester linkages)- C 4 (hinge region)-T ι0 (phosphodiester linkages) does not form triplex with a polyA RNA target. Hinge regions can comprise any flexible linkage that keeps the two triplex forming regions together and allows them to associate with the third strand to form the triplex. Third strand targets are selected to have appropriate purine/pyrimidine content so as to allow formation of triplex molecules. The flexible linkage may connect the two triplex forming regions (typically, complementary DNA strands) in any selected orientation depending on the nature of the base sequence of the target. For example, the two triplex forming regions each have 5' and 3' ends, these ends can be connected by the flexible hinge region in the following orientations: 5' to 3', 3' to 5', 3' to 3', and 5' to 5'. Further, duplex DNA molecules containing at least one phosphoramidate linkage in each strand can be used as decoy molecules for transcription factors or DNA binding proteins (e.g., c-myb). Single-stranded DNA can also be used as a target nucleic acid for oligonucleotides of the present invention, using, for example, phosphoramidate intersubunit linkage-containing haiφin structures (e.g., Figure 6). Two phosphoramidate analog oligonucleotides can be selected for single-strand DNA target-directed binding. Binding of the two phosphoramidate analog strands to the single-strand DNA target results in formation of a triplex. C. PHARMACEUTICAL COMPOSITIONS. The present invention includes pharmaceutical compositions useful in antisense and antigene therapies. The compositions comprise an effective amount of N3'-*P5' phosphoramidate oligonucleotides in combination with a pharmaceutically acceptable carrier. One or more N3'→P5' phosphoramidate oligonucleotides (having different base sequences) may be included in any given formulation. The N3'→P5' phosphoramidate oligonucleotides, when employed in therapeutic applications, can be formulated neat or with the addition of a pharmaceutical carrier. The pharmaceutical carrier may be solid or liquid. The formulation is then administered in a therapeutically effective dose to a subject in need thereof. Liquid carriers can be used in the preparation of solutions, emulsions, suspensions and pressurized compositions. The N3'→P5' phosphoramidate oligonucleotides are dissolved or suspended in a pharmaceutically acceptable liquid carrier such as water, an organic solvent, a mixture of both, or pharmaceutically accepted oils or fats. The liquid carrier can contain other suitable pharmaceutical additives including, but not limited to, the following: solubilizers, suspending agents, emulsifiers, buffers, thickening agents, colors, viscosity regulators, preservatives, stabilizers and osmolarity regulators. Suitable examples of liquid carriers for parenteral administration of N3'-*P5' phosphoramidate oligonucleotides preparations include water (partially containing additives, e.g., cellulose derivatives, preferably sodium carboxymethyl cellulose solution), alcohols (including monohydric alcohols and polyhydric alcohols, e.g., glycols) and their derivatives, and oils (e.g., fractionated coconut oil and arachis oil). For parenteral administration of N3'→P5' phosphoramidate oligonucleotides the carrier can also be an oily ester such as ethyl oleate and isopropyl myristate. Sterile carriers are useful in sterile liquid form compositions for parenteral administration. Sterile liquid pharmaceutical compositions, solutions or suspensions can be utilized by, for example, intraperitoneal injection, subcutaneous injection, intravenously, or topically. For example, antisense oligonucleotides directed against retinal cytomegalovirus infection may be administered topically by eyedrops. N3'→P5' phosphoramidate oligonucleotides can be also be administered intravascularly or via a vascular stent impregnated with mycophenolic acid, for example, during balloon catheterization to provide localized anti- restenosis effects immediately following injury. The liquid carrier for pressurized compositions can be halogenated hydrocarbon or other pharmaceutically acceptable propellent. Such pressurized compositions may also be lipid encapsulated for delivery via inhalation. For administration by intranasal or intrabronchial inhalation or insufflation, N3'→P5' phosphoramidate oligonucleotides may be formulated into an aqueous or partially aqueous solution, which can then be utilized in the form of an aerosol, for example, for treatment of infections of the lungs like Pneumocystis carnii. N3'→P5' phosphoramidate oligonucleotides may be administered topically as a solution, cream, or lotion, by formulation with pharmaceutically acceptable vehicles containing the active compound. For example, for the treatment of genital warts. The N3'→P5' phosphoramidate oligonucleotides may be administered in liposome carriers. The use of liposomes to facilitate cellular uptake is described, for example, in U.S. Patent Nos. 4,897,355 (Eppstein, D., et al, issued 30 Jan 1990) and 4,394,448 (Szoka, F,, et al, issued 19 July 1983). Numerous publications describe the formulation and preparation of liposomes. The dosage requirements for treatment with N3'→P5' phosphoramidate oligonucleotides vary with the particular compositions employed, the route of administration, the severity of the symptoms presented, the form of N3'→P5' phosphoramidate oligonucleotides and the particular subject being treated. In general, N3'-»P5' phosphoramidate oligonucleotides are administered at a concentration that affords effective results without causing any harmful or deleterious side effects (e.g., an effective amount). Such a concentration can be achieved by administration of either a single unit dose, or by the administration of the dose divided into convenient subunits at suitable intervals throughout the day. D. DIAGNOSTIC APPLICATIONS The phosphoramidate analog oligonucleotides are also useful in diagnostic assays for detection of RNA or DNA having a given target sequence. In one general application, the phosphoramidate analogs are labeled (e.g., isotopically or other detectable reporter group) and used as probes for DNA or RNA samples that bound to a solid support (e.g., nylon membranes). Alternatively, the phosphoramidate analog oligonucleotides may be bound to a solid support (for example, magnetic beads) and homologous RNA or DNA molecules in a sample separated from other components of the sample based on their hybridization to the immobilized phosphoramidate analogs. Binding of phosphoramidate analogs to a solid support can be carried out by conventional methods. Presence of the bound RNA or DNA can be detected by standard methods, for example, using a second labeled reporter or polymerase chain reaction (Mullis; Mullis, et al). Diagnostic assays can be carried out according to standard procedures, with suitable adjustment of the hybridization conditions to allow phosphoramidate analog hybridization to the target region. The ability of phosphoramidate analog oligonucleotides to bind at elevated temperature can also help minimizes competition for binding to a target sequence between the phosphoramidate analog probe and any corresponding single-strand phosphodiester oligonucleotide that is present in the diagnostic sample. E. OTHER APPLICATIONS In one aspect, the phosphoramidate analog oligonucleotides can be used in methods to enhance isolation of RNA or DNA from samples. For example, as discussed above, phosphoramidate analogs can be fixed to a solid support and used to isolate complementary nucleic acid sequences, for example, purification of a specific mRNA from a polyA fraction (Goldberg, et al). The phosphoramidate analogs are advantageous for such applications since they can form more stable interactions with RNA and duplex DNA than standard phosphodiester oligonucleotides. A large number of applications in molecular biology can be found for reporter labeled phosphoramidate analogs, particularly for the detection of RNA in samples. Phosphoramidate analogs can be labeled with radioactive reporters ( 3 H, 14 C, 32 P, or 35 S nucleosides), biotin or fluorescent labels (Gryaznov, et al). Labelled phosphoramidate analog oligonucleotides can be used as efficient probes in, for example, RNA hybridization reactions (Ausubel, et al., Sambrook, et al). Also, double-stranded DNA molecules where each strand contains at least one phosphoramidate linkage can be used for the isolation of DNA-duplex binding proteins. In this embodiment the duplex containing phosphoramidate intersubunit linkages is typically affixed to a solid support and sample containing a suspected binding protein is then passed over the support under buffer conditions that facilitate the binding of the protein to its DNA target. The protein is typically eluted from the column by changing buffer conditions. The triplex forming DNA molecules described above, containing phosphoramidate modified linkages, can be used as diagnostic reagents as well, to, for example, detect the presence of an RNA molecule in a sample. Further, complexes containing oligodeoxyribonucleotides having N3'→P5' phosphoramidate intersubunit linkages can be used to screen for useful small molecules or binding proteins: for example, N3'-»P5' phosphoramidate oligodeoxyribonucleotide complexes with duplex DNA can be used to screen for small molecules capable of further stabilizing the triplex structure. Similar screens are useful with N3'→P5' phosphoramidate oligodeoxyribonucleotide complexes formed with single strand DNA and RNA molecules. F. VARIATIONS Variations on the phosphoramidate analog oligonucleotides used in the methods of the present invention include modifications to facilitate uptake of the oligonucleotide by the cell (e.g., the addition of a cholesterol moiety (Letsinger, 1990); production of chimeric oligonucleotides using other intersubunit linkages (Goodchild); modification with intercalating agents (for example, triplex stabilizing intercalating agents, Wilson, et al, 1993); and use of ribose instead of deoxyribose subunits. Further modifications include, 5' and 3' terminal modifications to the oligonucleotides (e.g., -OH, -OR, -NHR, NH 2 and cholesterol). In addition, the ribose 2' position can be the site of numerous modifications, including, but not limited to, halogenation (e.g., -F). N3'→P5' phosphoramidate oligonucleotides may also be modified by conjugation to a polypeptide that is taken up by specific cells. Such useful polypeptides include peptide hormones, antigens and antibodies. For example, a polypeptide can be selected that is specifically taken up by a neoplastic cell, resulting in specific delivery of N3'→P5' phosphoramidate oligonucleotides to that cell type. The polypeptide and oligonucleotide can be coupled by means known in the art (see, for example, PCT International Application Publication No. PCT/US89/02363, WO8912110, published 12/14/89, Ramachandr, K, et al). The properties of such modified phosphoramidate analog oligonucleotides, when applied to the methods of the present invention, can be determined by the methods described herein. While preferred embodiments, uses, and methods of practicing the present invention have been described in detail, it will be appreciated that various other uses, formulations, and methods of practice as indicated herein are within the contemplation of the present invention. MATERIALS AND METHODS The methyl and cyanoethyl phosphoramidates and the H-phosphate nucleoside reagents were purchased from Glen Research (Sterling, VA) and Applied Biosystems Inc., (Foster City, CA) Nucleoside methylphosphonoamidite reagents were purchased from Glen Research, and DMT-dT-LCAA CPG, 500 A, was purchased from Applied Biosystems Inc. For enzymatic hydrolysis of oligonucleotides, 0.2 A^ o units of a selected oligonucleotide and 0.22 U of phosphodiesterase from Crotalus durissus (Boehringer- Mannheim, Indianapolis, IN) were incubated for in 100 μl 10 mM Tris*HCl and 10 mM MgCl 2 . Samples were taken for analysis at 0', 10', 40', 4.5 hours and 22 hours after addition of the phosphodiesterase. Products were analyzed by RP HPLC essentially as described in Example 2D. Standard nucleic acid chemistry, including chemical synthesis of nucleic acids, has been reviewed by Miller (1990). Chemicals were purchased from Aldrich (Milwaukee, WI), Sigma (St. Louis, MO) and Calbiochem (San Diego, CA). HPLC was typically carried out using a Dionex chromatograph (Sunnyvale, CA). A "HYPERSIL ODS" column (4.6 x 200 mm, 5 μ particle size; Hewlett Packard, Palo Alto, CA) and a 0.5%/minute gradient of CH 3 CN in 0.05 M TEAH buffer, pH 7.0, were used for RP HPLC. For ion exchange chromatography, a Dionex "OMNI PAK" NA 100 column (4 x 250 mm) was used with a 1 %/minute gradient of 1.5 M NaCl in water. "NAP 5" columns from Pharmacia (Uppsala, Sweden) were used for desalting of oligonucleotides. Capillary electrophoresis (CE) analysis was performed on an ABI 270A system with 10% MICROGEL™ capillaries (0.1 x 500 mm) in 35 mM Tris-borate buffer, pH 9.0. Thermal dissociation experiments were done on a Varian IE spectrophotometer and temperature controller. Absorbance values at 260 nm or 284 nm were obtained at 1 minute intervals at a heating rate of 1.0°C/minute. EXAMPLE 1 SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING OLIGODEOXYRIBONUCLEOTIDE N3'-*P5' PHOSPHORAMIDATES A. GENERAL METHODS. Synthesis of the phosphoramidate analogs was carried out either manually in syringe or automatically on ABI 384 synthesizer (ABI, Foster City, CA). A schematic representation of the synthesis of uniformly modified oligonucleotides on a solid support using a step-by-step elongation procedure is shown below: Wherein R is succinyl CPG (control pore glass), B is a base, n is 4 to 100 and CEO is -cyanoethyl group For a given cycle the chemical steps, reagents, and reaction times were (i) detritylation, 3 % dichloroacetic acid in dichloromethane, 1.5 min. (Figure 1 , step i); (ii) phosphitylation, 0.2 M 2-cyanoethyl-N,N-diisopropylchlorophospine and 0.2 M diisopropylethylamine in dichloromethane, 10 min. (Figure 1, steps ii); (iii) hydrolysis, 0.4 M tetrazole in acetonitrile/water, 9/1 v/v, 5 min. (Figure 1, step iii); (iv) coupling, 0.2 M 5'-DMT-3'-amino nucleoside and 0.2 M triethylamine in carbon tetrachloride/acetonitrile, 1/1, v/v, 20 min (Figure 1, step iv). Standard oligonucleotides have phosphodiester intersubunit linkages were synthesized by standard methods (ABI 384 synthesizer). To construct chimeric oligomers, 5'-DMT-N-protected 3 '-phosphoramidate dimer building blocks, having a 3'-NHP(O)(OCE)O-5' phosphoramidate internucleoside linkage group, were used for synthesis with the conventional phosphoramidate method (essentially as previously described by Gryaznov, et al, herein incoφorated by reference). Exemplary oligonucleotides synthesized by the method of the present invention are presented in Figure 3. Further details of synthesis follow here. B. MANUAL SYNTHESIS OF THE OLIGONUCLEOTIDE N3'→P5' PHOSPHORAMIDATES Controlled pore glass (CPG) polymer support containing 1 μmol of 5'-DMT-N- protected nucleoside was placed in a 1 ml Hamilton gas tight syringe equipped with a plug of glass wool at the base. For a given cycle of synthesis, reagents were drawn in and expelled from the syringe according to the following protocol: 1. Detritylation — 3 % dichloroacetic acid in dichloromethane, 5 x 0.5 ml: 1,5 min. 2. Washing — acetonitrile, 6 x 0.5 ml. 3. Phosphitilation — 0.2 M 2-cyanoethyl-N,N-diisopropylchlorophospine and 0.2 M diisopropylethylamine in dichloromethane, 0.5 ml, 10 min with periodic shaking. 4. Hydrolysis — 0.4 M tetrazole in acetonitrile/water, 9/1, v/v, 5 minutes with periodic shaking. 5. Washing — anhydrous acetonitrile, 10 x 0.5 ml. 6. Coupling - 0.2 M 5'-DMT-3'amino nucleoside and 0.2 M triethylamine in carbon tetrachloride/acetonitrile, 1/1, v/v, 20 minutes, with shaking. After coupling, the solution was collected to recover un-reacted nucleoside. 7. Washing - acetonitrile, 6 x 0.5 ml. The steps 1-7 were repeated until the desired oligonucleotide was prepared. The average coupling yields were 94-96% as judged by DMT-cation assay. On completion of the cycles, the support-bound oligomer was detritylated. Cleavage from the support and N- deprotection with concentrated ammonium hydroxide afforded crude oligonucleotides which were purified by ion exchange HPLC. C. MANUAL SYNTHESIS OF THE OLIGONUCLEOTIDE 2 CONTAINING ALTERNATIVE N3'→P5' PHOSPHORAMIDATE O3'→P5' PHOSPHODIESTER LINKAGES 5'-DMT-3'-amino thymidine and 5'-DMT-thymidine-3'-phosphoramidate subunits were used to synthesize oligonucleotides having alternative N3'→P5' phosphoramidate and O3'→P5' phosphodiester linkages. For the synthesis of oligonucleotide 2 (SEQ ID NO:2), one micromole of T-CPG (Thymidine-linked-CPG) was placed in a 1 ml Hamilton gas tight syringe. Addition of 5'- DMT-3'-amino thymidine subunits was carried out as described above. Addition of 5'- DMT-thymidine-3 '-phosphoramidate subunits was carried out by standard synthetic procedures (Applied Biosystems, Foster City CA). After the 9th coupling reaction, the polymer support was treated with concentrated ammonia hydroxide to release the crude oligomer. The oligonucleotide was purified by ion exchange HPLC (e.g., Example 2). Oligonucleotide 2 was analyzed by CE and 31 P NMR (e.g. , Example 2). D. SYNTHESIS OF THE 5'-DMT-N-ISOBUTYRYL-3'-AMINO-2'.3'- DIDEOXYGUANOSINE The following steps describe a method of synthesis of 5'-DMT-N-isobutyryl-3'-amino- 2',3'-dideoxyguanosine, analogous to the method described herein for adenosine subunits. 1. 5 ' -O-BENZOYL-N-ISOBUTYRYL-2' -DEOXYGU ANOSINE was prepared from N-isobutyryl-2'-deoxyguanosine essentially according to the method of Nishino, et al (1986) the methods are herein incoφorated by reference, except that the product was purified as follows: partitioning between CH 2 C1 2 and water, concentrating the CH 2 C1 2 layer in vacuo, and crystallizing the product with ether. After recovery by filtration, the product was stirred overnight in fresh ether and recollected by filtration. The overall yield of 5'-O- benzoyl-N-isobutyryl-2'-deoxyguanosine was 50-80%. 2. 5'-O-BENZOYL-N-ISOBUTYRYL-2'-DEOXΎXYLOGU ANOSINE was prepared from 5'-O-benzoyl-N-isobutyryl-2'-deoxyguanosine essentially according to the method of Herdewijn and van Aerschot (1989) the methods are herein incoφorated by reference. The product was purified by drying the crude mixture in vacuo, then dissolving in CH 2 C1 2 . The 3 '-O-benzoyl-N-isobutyryl-2'-deoxyxloguanosine product spontaneously precipitated and was obtained by filtration. 3. 5 ' -Q-C4.4' -DIMETHOXYTRΓTΎL)-3 ' -O-BENZOYL-N-ISOBUTYRYL-2' - DEOXYXYLOGUANOSINE . Dry 3'-O-benzoyl-N-isobutyryl-2'-deoxyxyloguanosine (7.3 g) and 7.9 g 4,4'-dimethoxytrityl chloride were dissolved in 150 mL anhydrous pyridine. After 24 hours, 1 mL of water was added to the mixture. The mixture was then concentrated in vacuo to a foam containing the 5'-O-(4,4'-dimethoxytrityl)-3'-O-benzoyl-N- isobutyryl-2'-deoxyxyloguanosine product. The foam was dissolved in 300 mL CH 2 C1 2 , washed with 250 mL water, and reconcentrated in vacuo. 4. 5'-O-( ' 4.4'-DIMETHOXYTRΓΓYL -N-ISOBUTYRYL-2'- DEOXYXYLOGU ANOSINE . The crude 5'-O-(4,4'-dimethoxytrityl)-3'-O-benzoyl-N-isobutyryl- 2'-deoxyxyloguanosine was dissolved in 1.2 L 5:4: 1 dioxane:methanol: water and cooled in an ice bath. To this mixture, 120 mL 2 N NaOH was added. The resulting mixture was stirred at 0°C for 25 minutes, and neutralized with pyridinium H + -form Dowex 50 ion exchange resin. After 2-3 minutes, the resin was removed by filtration and the product concentrated to a slurry in vacuo. The white precipitate in the slurry (5'-O-(4,4'- dimethoxytrityl)-N-isobutyryl-2'-deoxyxyloguanosine) was removed by filtration, washed with water, air dried and desiccated under vacuum over P 2 O 5 . 5. 5'-O-(4.4'-DIMETHOXYTRITYL -3'-AMINO-N-ISOBUTYRYL-2'.3'- DIDEOXYGU ANOSINE. To the crude 5'-O-(4,4'-dimethoxytrityl)-N-isobutyryl-2'- deoxyxyloguanosine, 7.9 g triphenylphosphine and 4.8 g LiN 3 were added. The mixture was further dried under vacuum over P 2 O 5 for 3 hours. These dry compounds were dissolved in 450 mL anhydrous dimethylformamide. To this solution 5.3 mL diethyl azodicarboxylate was added. The mixture was stirred overnight, 1 mL water added, and the solvent removed in vacuo. One liter CH 2 C1 2 was added to the dried mixture and the resulting mixture washed twice with 1 L of water each time. The CH 2 C1 2 layer was concentrated in vacuo to a light brown oil which was then dissolved in 600 mL 10% triethylamine in pyridine. This mixture was cooled in an ice bath and H 2 S added by bubbling. After 30 minutes, the ice bath was removed, and the H 2 S stream continued another 3 hours. The solution was concentrated in vacuo to a light brown oil. Flash chromatography of the oil on a silica gel column, pretreated with 0.5% pyridine in CH 2 C1 2, then eluted with a gradient of 0-5% methanol in CH 2 C1 2 produced 3.5 g 5'-O- (4,4'-dimethoxytrityl)-3-amino-N-isobutyryl-2',3'-dideoxyguanosine (Figure IC). E. SYNTHESIS OF THE 5'-DMT-N-BENZOYL-3'-AMINO-2'.3'-DIDEOXΎADENOSINE The steps of the synthesis of 3'-amino-N 6 -5'-dimethoxytrityl-2',3'-dideooxy-adenosine are illustrated below: 1. PREPARATION OF N 6 5'-DIBENZOYL-2'-DEOXΎADENOSINE. A solution of benzoyl chloride (2.45 mL, 21 mmol) in pyridine (150 mL) was added drop-wise to a solution of N 6 -benzoyl-2'-deoxyadenosine (compound 1, Figure 20) (5 g, 14 mmol) in pyridine (45 mL) at room temperature over approximately 1 hour. The reaction mixture was stirred at room temperature for an additional hour. The reaction mixture was quenched with methanol (5 mL) and evaporated to dryness. The residue was dissolved in CH 2 C1 2 , washed with sat. aq. NaHCO 3 and H 2 O. The organic layer was then dried over Na 2 So 4 and evaporated to dryness. This residue was dissolved in CH 2 C1 2 and subjected to column chromatography (silica gel, 70-230 mesh, 200 g), eluted with 5% CH 3 OH/CH 2 Cl 2 , and the solvent removed by evaporation, yielding 6 g (93 %) of the desired N 6 5'-dibenzoyl-2'-deoxyadenosine product (compound 2, Figure 20). 2. PREPARATION OF N 6 -BENZoYL-9-(3'-O-BENZoYL-2'-DEoχγ-i8-D- THREO-PENTEOFURANOSYL) ADENINE. Trifluoromethane sulfonic anhydride (4.2 mL, 25 mmol), was added to the suspension of N 6 5'-dibenzoyl-2'-deoxyadenosine (7.5 g, 16.3 mmol) in 10% pyridine/CH 2 Cl 2 (150 ML) at 0°C. The reaction was stirred at 0°C for 30 minutes followed by the addition of H 2 O (26 mL). The reaction mixture was then stirred at room temperature overnight, and evaporated to dryness. The residue was redissolved in CH 2 C1 2 , washed with sat. aq. NaHCO 2 , H 2 O, and dried over NajSO,,. The organic layer was evaporated to dryness to give brown oil, containing the N 6 -benzoyl-9-(3-O-benzoyl-2-deoxy-0-D-THREO-PENTEOFURANOSYL) ADENINE product (compound 3, Figure 20), which was used without further purification. 3. PREPARATION OF N 6 -BENZOYL-9-(3'-O-BENZOYL-5'- DIMETHOXYTRΠΎL-2 ' -DEOXY-I3-D-THREO-PENTOFURANOS YL) ADENINE . N 6 -benzoyl-9-(3-O- benzoyl-2'-deoxy-/3-D-THREO-PENTEOFURANOSYL) ADENINE was dissolved in pyridine (60 mL). Dimethotytrityl chloride was then stirred at room temperature overnight. The reaction was quenched with CH 3 OH. The mixture was evaporated to dryness. The residue was redissolved in CH 2 C1 2 , washed with sat. aq. NaHCO 3 , H 2 O, dried over Na^Q, and evaporated. The residue was dissolved in CH 2 C1 2 and subjected to column chromatography (silica gel, 70-230 mesh, 400 g), eluted with 4% CH 3 OH/CH 2 Cl 2 . The desired fraction containing the N 6 -benzoyl-9-(3-O-benzoyl-5-dimethoxytrityl-2-deoxy-/3-D-THREO- PENTOFURANOSYL) ADENINE PRODUCT (COMPOUND 4, FIGURE 20) was collected and evaporated to dryness. The yield of product was 7 g (56% from N 6 5'-dibenzoyl-2'- deoxyadenosine). 4. PREPARATION OF N 6 -BENZθYL-9-f5'-O-DiMETHθXYTR * π * ΥL-2'-DEoχγ- <3-D-THREO-PENTOFURANOSYL) ADENINE). Aqueous NaOH (2 M, 40 mL) was added to a solution of N 6 -benzoyl-9-(3-O-benzoyl-5-dimethoxytrityl-2-deoxy-/3-D-THREO- PENTOFURANOSYL) ADENINE (7 g, 9.2 mmol) in a mixture of p-Dioxane (200 mL), CH 3 OH (160 ML) and H 2 O (40 mL) at 0°C. The reaction mixture was stirred at 0°C for 25 minutes. The reaction was terminated by the addition of Dowex 50 x 2-100 ion-exchange resin (pyridinium form) to neutralize the solution to pH 7. The solid was removed by filtration and the solvent evaporated to dryness. The residue was dissolved in CH 2 C1 2 , washed with sat. aq. NaHCO 3 , dried over Na 2 SO 4 and evaporated to dryness to give light yellow solid containing the N 6 -benzoyl-9-(5- O-dimethoxytrityl-2-deoxy-i3-D-THREθ-PENTθFURANθSYL) ADENINE PRODUCT (COMPOUND 5, FIGURE 20). This product was used without further purification. The yield of product from the previous step was 6 g (99%). 5. PREPARATION OF 3'-AMINO-N 6 -BENZOYL-5'-DIMETHOXΎTRΓΓΎL-2'.3'- DIDEOXY-ADENOSINE . Diethylazodicarboxylate (1.42 mL, 9 mmol) was added to a suspension of N 6 -benzoyl-9-(5-O-dimethoxytrityl-2-deoxy-3-D-THREθ-PENTθFURANθSYL) ADENINE) (2 g, 3 mmol) in DMF (40 mL), the suspension further containing triphenylphosphine (2.4 g, 9 mmol) and LiN 3 (4.1 g, 83.8 mmol). The reaction mixture was stirred at room temperature overnight and evaporated to dryness. The residue was dissolved in CH 2 C1 2 , washed with sat. aq. NaHCO 3 , H 2 O, dried over and evaporated to give a brown oil. The brown oil was dissolved in CH 2 C1 2 and subjected to column chromatography (silica gel, 70-230 mesh, 100 g), eluted with 3% CH 3 OH/CH 2 Cl 2 to give compound 6 (Figure 20) as light brown oil. This oil was dissolved in 15% triethylamine/pyridine (36 mL). Hydrogen sulfide was then bubbled into the solution at 0°C for 30 minutes. The solution was stirred at room temperature for 30 more minutes. The solvent was evaporated to dryness. The residue was dissolved in CH 2 C1 2 , washed with sat. aq. NaHCO 2 , H 2 O, dried over Na 2 SO 4 and evaporated to dryness. The residue was dissolved in CH 2 C1 2 and subjected to column chromatography (silica gel, 70-230 mesh, 100 g), eluted with 5% CH 3 OH/CH 2 Cl 2 to give the final product 3'-amino-N 6 -5'-dimethoxytrityl-2',3'-dideooxy-adenosine (compound 7, Figure 20; Figure le). The yield of the product from the previous step was 1.6 g (80%). Alternatively, reduction can be accomplished using H 2 over Pt/carbon catalyst. F. SYNTHESIS OF OTHER DMT-SUBUNΓTS Synthesis of the 5'-DMT-3'amino thymidine and 5'-DMT-N-benzoyl-3'-amino cytidine was performed according to Glinski, et al. (1970). EXAMPLE 2 CHARACTERIZATION OF OLIGONUCLEOTIDES CONTAINING N3'→P5' PHOSPHORAMIDATE LINKAGES Oligonucleotides synthesized as described in Example 1 were evaluated by the following methods. A. PURIFICATION BY ION EXCHANGE CHROMATOGRAPHY Oligonucleotides were purified away from excess reaction components by ion exchange (IE) HPLC. IE HPLC analyses were performed on a Dionex (Sunnyvale, CA) chromatograph. A Dionex "OMNIPAC NA100," 4 x 250 mm column was used, with a 1 %/min or 2%/min gradient of 1.0 M NaCl in 0.03 M TEAA buffer, pH 7.0; flow rate, 1.0 ml/min. Figure 4A presents an exemplary HPLC chromatogram of the reaction mixture after synthesis of the phosphoramidate Oligonucleotide 3 (Figure 3). The largest peak in the figure corresponds to the Oligonucleotide 3 product. Retention time of the 3'- NHP(O)(O " )O-5' phosphoramidates on IE HPLC column was 1.0-1.5 minutes shorter than the retention time for corresponding phosphodiester compounds The product was then concentrated by precipitation with ethanol and resuspended in water. B. PURITY OF OLIGONUCLEOTIDES Purity of synthesized oligonucleotides was typically evaluated by capillary gel electrophoresis. Capillary electrophoresis was performed using an Applied Biosystems Incoφorated Model 270A machine, on "MICKROGEL" capillary tubes, essentially following the directions of the manufacturer. Figure 4B presents an exemplary capillary gel electrophoresis profile of the reaction mixture after synthesis of the undecaphosphoramidate 6 (Figure 3). Alternatively, purity of the isolated oligonucleotides is evaluated by electrophoretic separation of the samples in high percent polyacrylamide gels, for example, 20% acrylamide, 5% bis-acrylamide (Ausubel, et al; Maniatis, et al). Oligonucleotides were visualized by staining with ethidium bromide and exposure to UV light. Relative mobilities in polyacrylamide gels upon electrophoretic separation of the 3'-NHP(O)(O " )O-5' phosphoramidates was 10-15% lower than for the corresponding phosphodiester compounds. C. NUCLEAR MAGNETIC RESONANCE ANALYSIS NMR spectra were recorded on a Varian XL-400 (Varian Associates, Palo Alto, CA) spectrometer at 162 MHz for 31 P spectra, with 85% phosphoric acid in D 2 O as an external standard, and at 400 MHz for Η spectra, with tetramethyl silane (TMS) as an external standard. Exemplary results are presented in Figure 4C, showing the 31 P-NMR spectrum of the purified decaphosphoramidate oligonucleotide 3 (Figure 3). The spectrum presents a peak at δ, ppm, 7.12 which is characteristic of phosphoramidate groups. D. REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF HYDROLYSIS PRODUCTS Purified phosphoramidate 10-mer oligonucleotide 3 (Figure 3) was hydrolysed by treatment with 80% acetic acid for 48 hours at 25°C. The hydrolysis products were evaluated by reverse phased (RP) HPLC. RP HPLC analyses were performed with a Dionex chromatograph on a "HYPERSIL ODS" 5 μ, 4.6 x 200 mm column from Hewlett Packard (Palo Alto, CA), using a 1 % min gradient of acetonitrile in 0.03 M TEAA buffer, pH 7.0; flow rate, 1 ml/min. The hydrolysis products were identified as 3' amino-5'- thymidilic acid, 5'-thymidilic acid, and 3'-aminothymidine. Further, about 7% of the hydrolysis products (total peaks) were minor by-products. These results confirm the presence of N3'→P5' phosphoramidate linkages in the oligonucleotide. EXAMPLE 3 THERMAL DISSOCIATION A. DUPLEX MELTING Thermal dissociation curves were obtained using a Carry IE spectrophotometer (Varian, Palo Alto, CA) that was equipped with a temperature controller (Varian). The reaction solutions contained equivalent concentrations of oligomer and complement (approximately 6 μM in oligomer strand) in 15 mM phosphate buffer at pH 7.05, with NaCl added to give a total Na+ concentration of 100 mM. The molar extinction coefficients used for oligo(dT), poly(dA) and poly(A) were 8.2, 8.4, and 10.2 A^o X 10 3 , respectively. An extinction coefficient of 109 A 260 Units/μM was used for the mixed-based oligomers — the extinction coefficient was calculated from the table compiled by P.N. Borer (1975). Reaction solutions were equilibrated at 0°C and the absorbance at 260 nm was followed as the temperature was increased in increments of 3°C per 5 minutes. The fraction of an oligomer in the bound state, a, at a given temperature was determined by use of upper and lower base lines as described by Abergo, et al. (1981). T m values are defined as the temperature at which a = 0.5. Plots of Ink (Marky, et al, 1987) versus 1/T were linear for these compounds. The results of the thermal denaturation studies were also plotted as normalized absorbance at 260 nm versus temperature in °C. Figures 5A and 5B display exemplary melting curves for the duplexes formed by phosphodiester and phosphoramidate oligomers. In the figures: (A),(C) and (B),(D), respectively, correspond to Figure 3, experiments 8, 9, using phosphodiester Oligonucleotide 4); and, Figure 3, experiments 13, 14 using phosphoramidate Oligonucleotide 6). The thermal stability data is summarized in Figure 3 {T m (°C)}. In the table, T m is the temperature at the midpoint of the melting curve; np is the abbreviation for the 3'-NHP(O)(O )0-5' phosphoramidate link. The concentrations of oligonucleotides were typically at 5 μM oligomer strands. Buffer A (10 mM Tris HC1, 150 mM NaCl, pH 7.02) was used for duplex thermal stability studies. B. OLIGONUCLEOTIDE HAIRPINS The stability of duplexes formed by oligonucleotides containing phosphoramidate linkages in both complementary strands was evaluated essentially as described above. The chimeric phosphoramidate-phosphodiester haiφin oligomers presented in Figure 6 were synthesized. All the molecules in Figure 6 were constructed using 2'-deoxyribonucleotides. Thermal dissociation experiments were performed essentially as described above, but with the following reaction conditions: 10 mM Tris HC1 buffer, pH 7.02 at oligonucleotide concentration 2.5 μM. The T m values obtained from these experiments are summarized in Figure 6. C. TRIPLEX MELTING Triplex thermal stabilities were evaluated essentially as described above for duplexes. Buffer conditions were either as described above (Buffer A) or a second buffer, Buffer B (10 mM Tris HC1, 150 mM NaCl, 10 mM MgCl 2 , pH 7.02.) was used. Figures 7A to 7D present exemplary triplex melting curves. Figures 7A and 7C present normalized absorbance at 260 nm plotted against temperature. Figures 7B and 7D present normalized absorbance at 284 nm plotted against temperature. In the figures: the triplexes formed by double stranded DNA and phosphoramidate Oligonucleotide 3 correspond to the black circle line; triplexes formed with phosphodiester Oligonucleotide 1 correspond to open squares line). The data correspond to Figure 3, experiment 7 where curves (A) and (C) were thermal stability studies performed using buffers A and B, respectively, with hyperchromicity monitored at 260 nm. Curves (B) and (D) are (A) and (C) respectively, but where hyperchromicity was monitored at 284 nm. The data presented in Figures 7A to 7D indicate that phosphodiester Oligonucleotide 1 did not form triplexes with the same double-stranded DNA targets as did phosphoramidate Oligonucleotide 3. EXAMPLE 4 GEL BAND MOBILITY SHIFT ASSAYS Triplex structures were further evaluated using gel band mobility shift assays. A. PHOSPHORAMIDATE OLIGONUCLEOTIDE 3 Gel band mobility shift assay conditions were as follows: 20% acrylamide, 5% bis- acrylamide in 10 mM MgCl 2 , 80 mM Tris-borate buffer, pH 8.2, 10°C. Gels were typically run under native (non-denaturing) conditions. Exemplary results of such a gel band mobility shift assay are presented in Figure 8. In the figure the lanes are as follows: 1 - 10-mer phosphodiester Oligonucleotide 1; 2 - 10-mer phosphoramidate Oligonucleotide 3; 3 - 24-mer haiφin target d(A 10 C 4 Tι 0 ) (Figure 3, experiment 7). In lane 3 the slow moving minor band likely corresponds to a bi-molecular duplex of d(Aι 0 C 4 T 10 ); 4 - haiφin target and Oligonucleotide 1; 5 - haiφin target and Oligonucleotide 3. The gel was stained with STAINS-ALL™ (Kodak, Rochester NY) and imaged on a Molecular Dynamics (Sunnyvale, CA) densitometer. The efficiency of the phosphoramidate Oligonucleotide 3 staining is different from phosphodiester compounds (Oligonucleotide 1 and the duplex). In the figure the mobility of the triplex structure is denoted by arrow. As can be seen from the results presented in the figure, the gel band mobility shift assay results confirm the results obtained from thermal denaturation studies, i.e. , that Oligonucleotide 1 fails to form a triplex with the same target as Oligonucleotide 3. B. PHOSPHORAMIDATE OLIGONUCLEOTIDE 6 The triplex formation of phosphoramidate Oligonucleotide 6 analog with a double- stranded DNA target was also confirmed by gel band mobility shift assay. The conditions of the gel band mobility shift assay were essentially as described above. The results of the analyses are presented in Figure 9. In the figure the lanes were as follows: 1 - 11-mer phosphodiester Oligonucleotide 4; 2 - 11-mer phosphoramidate Oligonucleotide 6; 3 - 26-mer haiφin target (SEQ ID NO: 22); 4 - haiφin target and Oligonucleotide 4; 5 - haiφin target and Oligonucleotide 6. In the figure the mobility of the triplex structure is denoted by arrow. As seen above, the phosphodiester Oligonucleotide 4 fails to form a triplex with the same target as Oligonucleotide 6. EXAMPLE 5 IN VΓΓRO EVALUATION OF PHOSPHORAMIDATE ANALOGS N3'-*P5' phosphoramidate linkage containing anti-sense oligonucleotides were synthesized which were complementary to the BCR/ ABL fusion junction (B2A2) in leukemic cell line BV173. The following assay measures leukemic cell proliferation and is performed essentially as described by Anfossi, et al. (1989). Briefly, Figure 10 illustrates the effect of BCR-ABL oligomers on leukemic cell proliferation of BV173 cells carrying the B2A2-type break points. BV173 is a leukemic cell line with the B2A2 BCR/ ABL fusion junction (Pegoraro, et al, 1983). HL60 is a promyelocytic leukemic cell line with a normal c-abl locus (Collins, et al, 1977). K562 is a leukemic cell line with the B3A2 BCR/ ABL fusion junction (Seelig, et al, 1993). Cells (5 x 10 4 ) were placed in 0.2 ml of liquid suspension culture (Iscove's modified Dulbecco's modified medium with 2% human AB serum - Life Technologies, Gaithersburg, MD). The oligonucleotides were administered to the cultures at 24 hour intervals for three days (days 0, 1 and 2) to achieve the incremental concentrations shown in the legends of Figures 10-19 (all concentrations in μg/mL), for example, Figure 10 — 40/20/20; 20/10/10; 10/5/5; and 5/2.5/2.5. Note that, for example, 40/20/20 reflects a final total concentration of 80 μg/ml in the cell culture medium after the last oligonucleotide addition. Cell counts were performed by standard methods. Figure 10 shows the results of the assay using fully modified N3'→P5' phosphoramidate oligonucleotide 6 (SEQ ID NO:6) at the concentrations shown in the figure legend. The target cells were BV173 cells. The results demonstrate that the phosphoramidate oligonucleotide is extremely effective at inhibiting the growth of the leukemia cells — even at the lowest concentration used. Figure 11 shows the results of treatment of the HL60 cells, a non-BRC-ABL expressing cell line, with oligonucleotide 6. The results demonstrate that at all concentrations the oligonucleotide was well tolerated by the cells. There was no apparent cytotoxicity. Figures 12 and 13 show the results of similar experiments to the results presented in Figures 10 and 11, respectively. Even at the lowest concentrations (0.3125/0.15625/0.15625) the N3'→P5' phosphoramidate oligonucleotide 6 is extremely effective at inhibiting leukemia cell proliferation (Figure 12) while maintaining negligible toxicity (Figure 13). Figures 14 and 15 show the results of similar experiments to the results presented in Figures 10 and 11, respectively. However, in the experiment corresponding to the data presented in Figure 15 the target cell was K562, which contains a B3A2 BCR/ ABL fusion junction. In the data from the experiment presented in Figure 14, transition concentrations for the effectiveness of the N3'-*P5' phosphoramidate oligonucleotide 6 in culture are shown. Specifically, at a concentration of 0.0198/0.0098/0.0098 some release of inhibition of proliferation can be seen with oligonucleotide 6. Further, the data presented in Figure 15 demonstrate that phosphoramidate oligonucleotide 6 had essentially no effect on the proliferation of K562 cells, having the B3A2 fusion junction. These results demonstrate that the antiproliferation effect is specifically associated with the B2A2 BCR/ ABL fusion junction to which phosphoramidate oligonucleotide 6 is complementary. In comparison, Figures 16-19 present similar data for a 16-mer (SEQ ID NO:26) having fully modified phosphorothioate intersubunit linkages. Figure 16 shows the results of the assay using fully modified phosphorothioate 16-mer (SEQ ID NO:26) at the concentrations shown in the figure legend where the target cell line was BV173. The results are similar to those seen with oligonucleotide 6 (Figure 10), but the data demonstrate that the phosphorothioate oligonucleotide is not as effective as oligonucleotide 6 at inhibiting the growth of the leukemia cells. Figure 17 shows the results of treatment of the BV173 BRC/ABL cell line with a 16- mer phosphorothioate oligonucleotide (SEQ ID NO:27) having a sequence complementary to the B3A2 BCR/ ABL fusion junction: B3A2 has a 2 base pair sequence mismatch to the B2A2 BCR/ ABL fusion junction. The results demonstrate that at all concentrations the oligonucleotide was tolerated by the cells. Figures 18 and 19 show the results of similar experiments to the results presented in Figures 16 and 17, respectively. In the data from these experiments, the release of the inhibition of cellular proliferation is seen at much lower concentrations for the phosphorothioate oligonucleotide than was seen for the N3'→P5' phosphoramidate oligonucleotide 6. These results demonstrate the in vitro N3'→P5' phosphoramidate oligonucleotides are effective antisense compounds at low concentrations. Further, the N3'→P5' phosphoramidate oligonucleotides are better antisense agents than phosphorothioate oligonucleotides. EXAMPLE 6 ANTISENSE INHIBITION WITH c-mvc N3'→P5' phosphoramidate oligonucleotides complementary to human c-myc mRNA were also used to inhibit c-myc gene expression and the effectiveness of N3'→P5' phosphoramidate oligonucleotide was compared to phosphorothioate oligonucleotides. HL60 cells were incubated with one of the following c-myc oligonucleotides: SEQ. ID NO. 28 TTTCATTGTT TTCCA (Antisense) SEQ. ID NO. 29 TTTCTATTGT TTCCA (Negative control; mismatch) S SEEQQ.. I IDD NNOO.. 3 300 AACGTTGAGG GGCAT (Antisense; second exon) SEQ. ID NO. 31 AACGAGTTGG GGCAT (Negative control; mismatch) SEQ. ID NO. 32 UUUCAUUGUU UUCCA (Antisense; T's replaced with U'ε) All internucleotide linkages of the c-myc oligonucleotides were N3'→P5' phosphoramidate linkages unless otherwise indicated. After each type of c-myc oligonucleotide was individually incubated at the indicated concentrations for 72 hours in culture with HL-60 cells, the cells were assayed either for proliferation by microscopy techniques known in the art or for c-myc expression by Western blot as described in Holt et al., Mol. and Gen Bio. 8:963-973(1988), the methods of which are incoφorated by reference. The results are shown in Table 3: TABLE 3 Improved Inhibition of c-myc expression in HL60 cells using N3'→P5' phosphoramidate oligonucleotides compared to phosphorothioate oligonucleotides Seq. ID No. Description Oligonucleotide % of "no DNA" Cone. Control (Western Blot Densitometry) no oligo O μM 100% 31 thioate negative control 10 μM 85% of control 30 thioate antisense 10 μM 35% of control 31 amidate negative control 1 μM 81 % of control 30 amidate antisense 1 μM 38% of control 28 amidate antisense 1 μM 38% of control 32 amidate antisense with 1 μM 33 % of control U's replacing T's N3'→P5' phosphoramidate oligonucleotide SEQ ID No. 29 also showed progressively more inhibition of H60 cell proliferation at .1 μM, .5 μM and 1 μM (where proliferation was measured at each concentration at day 0, 3 and 6), compared to N3'→P5' phosphoramidate oligonucleotide 31 (negative control; mismatch) at .1 μM, .5 μM and 1 μM, which showed proliferation levels comparable to the no DNA control. At day zero each culture had approximately 10,000 cells. By day six both control cultures had approximately 50,000 cells per culture and the N3'→P5' phosphoramidate 1 μM culture had approximately 25,000 cells per culture. This results demonstrate the ability of c-myc N3'→P5' phosphoramidate oligonucleotides to inhibit cell proliferation in dose and sequence dependent manner and the greater effectiveness of N3'→P5' phosphoramidate oligonucleotides compared to phosphorothioate oligonucleotides in inhibiting c-myc expression. This data correlates with the c-myc inhibition shown by Western Blot. While the invention has been described with reference to specific methods and embodiments, it will be appreciated that various modifications and changes may be made without departing from the invention. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Gryaznov, Sergei et al . (ii) TITLE OF INVENTION: Oligonucleotide N3'-P5' Phosphoramidates : Hybridization and Nuclease Resistance Properties (iii) NUMBER OF SEQUENCES: 31 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Cooley Godward (B) STREET: 5 Palo Alto Square (C) CITY: Palo Alto (D) STATE: CA (E) COUNTRY: USA (F) ZIP: 94306 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Paten In Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Mendlein, John D. (B) REGISTRATION NUMBER: 38,770 (C) REFERENCE/DOCKET NUMBER: Lynx-005/01W0 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (415) 843-5020 (B) TELEFAX: (415) 857-0663 (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 1, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: 10 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 2, Fig. 2 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..2 (D) OTHER INFORMATION: /note= "where the intersubunit bond (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 3..4 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 5..6 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 7..8 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 9..10 (D) OTHER INFORMATION: /note= "where the intersubunit bond (xi ) SEQUENCE DESCRIPTION : SEQ ID NO : 2 : 10 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 11 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 3, Fig. 2 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..11 (D) OTHER INFORMATION: /note= "where the intersubunit bonds are "np"" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: TTTTTTTTTT T 11 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 11 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 4, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: CTTCTTCCTT A 11 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 11 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Oligonucleotide 5, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: CTTCTTCCTT A 11 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 11 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 6, Fig. 2 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..11 (D) OTHER INFORMATION: /note= "where the intersubunit bonds are "np"" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: CTTCTTCCTT A 11 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA ( iii ) HYPOTHETICAL : NO ( iv) ANTI - SENSE : NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 7, Fig. 5 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: TATATATATA TTTTTATATA TATA 24 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 8, Fig. 5 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..2 (D) OTHER INFORMATION: /note= "where the intersubunit bond (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 3..4 (D) OTHER INFORMATION: /note= "where the intersubunit bond is " »nnnp'" " (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 5.-6 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 7..8 (D) OTHER INFORMATION: /note= "where the intersubunit bond (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: TATATATATT TTTATATATA 20 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 9, Fig. 5 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..2 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np' (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 3..4 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" ( ix) FEATURE : (A) NAME/KEY: misc_feature (B) LOCATION: 5..6 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 7..8 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 9..10 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 15..16 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 17..18 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 19..20 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np' (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 21..22 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 23..24 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: TATATATATA TTTTTATATA TATA 24 (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 10, Fig. 5 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: TACGTACGTA TTTTTACGTA CGAT 24 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 11, Fig. 5 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..2 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 3..4 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 5..6 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 7..8 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 9..10 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: TACGTACGTA TTTTTACGTA CGTA 24 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Oligonucleotide 12, Fig. 5 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..2 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 3..4 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np' (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 5..6 (D) OTHER INFORMATION: /note= "where the intersubunit bond (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 7..8 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 9..10 (D) OTHER INFORMATION: /note= "where the intersubunit bond (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 15..16 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" ( ix) FEATURE : (A) NAME/KEY: misc_feature (B) LOCATION: 17..18 (D) OTHER INFORMATION: /note= "where the intersubunit bond (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 19..20 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 21..22 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 23..24 (D) OTHER INFORMATION: /note= "where the intersubunit bond is "np"" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: TACGTACGTA TTTTTACGTA CGTA 24 (2) INFORMATION FOR SEQ ID NO:13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Target, Experiment 7, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: AAAAAAAAAA CCCCTTTTTT TTTT 24 (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Target, Experiment 8, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: ATAAGGAAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Target, Experiment 9, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: AUAAGGAAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Target, Experiment 10, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: AUAAGGUAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Target, Experiment 11, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: AUAAGGAAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Target, Experiment 12, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: AUAAGGUAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear t (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Target, Experiment 13, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19: ATAAGGAAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Target, Experiment 14, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: AUAAGGAAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: RNA Target, Experiment 15, Fig. 2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: AUAAGGUAGA AGC 13 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: DNA Target Duplex, Fig. 8 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22: TTCCTTCTTT CTTTTGAAAG AAGGAA 26 (2) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 244 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (genomic) (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: HIV-1 REV RESPONSE ELEMENT (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23: CAGUGGGAAU AGGAGCUUUG UUCCUUGGGU UCUUGGGAGC AGCAGGAAGC ACUAUGGGCG 60 CAGCGUCAAU GACGCUGACG GUACAGGCCA GACAAUUAUU GUCUGGUAUA GUGCAGCAGC 120 AGAACAAUUU GCUGAGGGCU AUUGAGGCGC AACAGCAUCU GUUGCAACUC ACAGUCUGGG 180 GCAUCAAGCA GCUCCAGGCA AGAAUCCUGG CUGUGGAAAG AUACCUAAAG GAUCAACAGC 240 UCCU 24 (2) INFORMATION FOR SEQ ID NO:24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 69 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (genomic) (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: STEM II OF THE HIV-1 REV RESPONSE ELEMENT (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24: AGCACUAUGG GCGCAGCGUC AAUGACGCUG ACGGUACAGG CCAGACAAUU AUUGUCUGGU 6 AUAGUGCAG 6 (2) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 58 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (genomic) (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: the sequence of the TAR site of HIV-1 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25: GGUCUCUCUG GUUAGACCAG AUCUGAGCCU GGGAGCUCUC UGGCUAACUA GAGAACCC 58 (2) INFORMATION FOR SEQ ID NO:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: 16-mer, phosphorothioate intersubunit linkages (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26: AAGGGCTTCT TCCTTA 16 (2) INFORMATION FOR SEQ ID NO:27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (C) INDIVIDUAL ISOLATE: 16-mer, phosphorothioate intersubunit linkages (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27 GAGTTCAAAA GCCCTT 1 (2) INFORMATION FOR SEQ ID NO:28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: both (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) SEQUENCE DESCRIPTION: SEQ. ID NO:28: TTTCATTGTT TTCCA 1 (2) INFORMATION FOR SEQ. ID NO:29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) SEQUENCE DESCRIPTION: SEQ. ID NO:29: TTTCTATTGT TTCCA 1 (2) INFORMATION FOR SEQ. ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 bases pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) SEQUENCE DESCRIPTION: SEQ. ID NO:30: AACGTTGAGG GGCAT 1 (2) INFORMATION FOR SEQ. ID NO:31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 bases pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (iii) SEQUENCE DESCRIPTION: SEQ. ID NO:31: AACGAGTTGG GGCAT 15

Claims: IT IS CLAIMED: 1. A nucleoside subunit comprising of the formula: where DMT is a dimethoxytrityl group and Bz is a benzoyl group. 2. A nucleoside subunit comprising the formula: where DMT is a dimethoxytrityl group, and IBU is an isobutyryl group. 3. A method for synthesizing 5'-DMT-N 6 -benzoyl-3'amino- 2',3'dideoxyadenosine or 5'-DMT-N 2 -isobutyryl-3'-amino-2',3'-dideoxyguanosine, comprising: 5' benzoylation of X, inversion of a 3' hydroxygroup, tritylation, 3' debenzoylation, transformation of a 3' threo azido group to a 3' erythro azido group, and reduction of said 3' erythro azido group, wherein X is either 5'-O-benzoyl-N-isobutyryl-2'-deoxyguanosine or N 6 -benzoyl-2'-deoxyadenosine. 4. An oligodeoxyribonucleotide comprising, an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, of the formula: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, where no R contains a carbon chain longer than 10 carbons, said intersubunit linkages are interspersed in an oligonucleotide at least 6 to 100 bases in length, n is from 4 to 100 and B is a base. 5. A oligodeoxyribonucleotide of claim 4, where said oligodeoxyribonucleotide has at least 3 contiguous subunits joined by N3'→P5' phosphoramidate linkages. 6. A oligodeoxyribonucleotide of claim 5, where said oligodeoxyribonucleotide has OH groups at 5' and 3' ends, and said contiguous linkages start at the 3' end nucleoside subunit. 7. A oligodeoxyribonucleotide of claim 4, where all of the intersubunit linkages are N3'→P5' phosphoramidate linkages. 8. A oligodeoxyribonucleotide of claim 4, where the intersubunit linkages alternate the N3'→P5' phosphoramidate linkage and a second linkage. 9. A oligodeoxyribonucleotide of claim 5, where at least 50% of the intersubunit linkages are N3'→P5' phosphoramidate linkages. 10. A oligodeoxyribonucleotide of claim 8, where the second linkage is are selected from the group consisting of phosphodiester, phosphotriester, methylphosphonate, phosphoramidate P3'→N5', and phosphorothioate. 11. A oligodeoxyribonucleotide of claim 10, where said second linkage is a phosphodiester linkage. 12. A method for generating a duplex nucleic acid molecule, comprising: forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 3 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunits, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages, as follows: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, said oligodeoxyribonucleotide having a sequence of nucleoside subunits effective to form a duplex with a target nucleic acid molecule, and contacting said oligodeoxyribonucleotide with said target nucleic acid molecule under conditions to allow formation of a duplex between said oligodeoxyribonucleotide and said target nucleic acid molecule. 13. A method for blocking an interaction of a protein with a polynucleotide, comprising: forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages, as follows where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, said oligodeoxyribonucleotide having a sequence of nucleoside subunits effective to form a complex with said polynucleotide, and contacting said oligodeoxyribonucleotide with said polynucleotide under conditions to allow formation of a complex between said oligodeoxyribonucleotide and said polynucleotide. 14. A method for generating a triplex molecule, comprising forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by A N3'-*P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages, as follows: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, said oligodeoxyribonucleotide having a sequence of nucleoside subunits effective to form triple-helix structure with a target duplex DNA, and contacting said oligodeoxyribonucleotide with said duplex DNA under conditions effective to allow formation of a triplex between the oligodeoxyribonucleotide and the duplex target DNA. 15. A method of claim 14, where 50% or greater of the intersubunit linkages are N3'→P5' phosphoramidate linkages. 16. A method of claim 14, where the intersubunit linkages alternate N3'→P5' phosphoramidate linkages and a second intersubunit linkages. 17. A method of claim 14, where the remaining intersubunit linkages are selected from the group consisting of phosphodiester, phosphotriester, methylphosphonate, phosphoramidate P3'→N5', and phosphorothioate. 18. A method of claim 14, where all of the intersubunit linkages are N3'→P5' phosphoramidate linkages. 19. A method of claim 15, where said contacting is carried out intracellularly. 20. A triplex molecule, having three nucleic acid strands, comprising a duplex DNA molecule, having two complementary strands, and bound to said duplex a third strand oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages as follows: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl. 21. A triplex molecule of claim 20, where 50% or greater of the intersubunit linkages of the third strand are N3'→P5' phosphoramidate linkages. 22. A triplex molecule of claim 20, where all of the intersubunit linkages of the third strand are N3'- P5' phosphoramidate linkages. 23. A method of enhancing the resistance of an oligodeoxyribonucleotide to nuclease digestion, comprising forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 3 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunits, or greater than 3 of the total intersubunit linkages are N3'-*P5' phosphoramidate intersubunit linkages as follows: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, and exposing said oligodeoxyribonucleotide to nuclease, where said oligodeoxyribonucleotide is more resistant to nuclease digestion than a corresponding oligodeoxyribonucleotide having only phosphodiester intersubunit linkages. 24. A method of claim 23, where 50% or greater of the intersubunit linkages of the oligodeoxyribonucleotide are N3'→P5' phosphoramidate linkages. 25. A method of claim 23, where all of the intersubunit linkages of the oligodeoxyribonucleotide are N3'→P5' phosphoramidate linkages. 26. A method of claim 23, where said exposing is carried out intracellularly. 27. A kit for the isolation of a target nucleic acid from a sample, comprising: (i) an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by N3'-*P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages as follows: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, (ii) said oligodeoxyribonucleotide is effective to hybridize to said target nucleic acid sequence, and (iii) said oligodeoxyribonucleotide is attached to a solid support. 28. A method to detect a nucleic acid having target sequence in a sample, comprising: forming an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where at least 3 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunits, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages as follows: where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, and said oligodeoxyribonucleotide is effective to form a hybridization complex with said target sequence, contacting said oligodeoxyribonucleotide with said sample under conditions effective to allow formation of the hybridization complex between the oligodeoxyribonucleotide and the target sequence, and detecting the presence of the hybridization complex. 29. A method of claim 28, where said oligodeoxyribonucleotide carries a reporter moiety and said detecting includes detection of said reporter moiety. 30. A method of claim 29, where said reporter moiety is selected from the group consisting of radioactive labels, biotin labels, and fluorescent labels. 31. A method of claim 28, where said contacting is carried out intracellularly. 32. A method of claim 28, where said nucleic acid is single-stranded. 33. A method to detect a duplex DNA having a target sequence in a sample, comprising forming an oligodeoxyribonucleotide having contiguous nucleoside subunits and intersubunit linkages, where (i) at least 3 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunits, or greater than 3 of the total intersubunit linkages are N3'-*P5' phosphoramidate intersubunit linkages as follows where X is -O " , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, and said oligodeoxyribonucleotide is effective to form a hybridization complex with said target sequence, contacting said oligodeoxyribonucleotide with said sample under conditions effective to allow formation of the hybridization complex between the oligodeoxyribonucleotide and the target sequence, and detecting the presence of the hybridization complex. 34. A method of claim 23, where said oligodeoxyribonucleotide carries a reporter moiety and said detecting includes detection of said reporter moiety. 35. A method of claim 23, where said hybridization complex is identified by a gel band shift. 36. A method of claim 23, where said contacting is carried out intracellularly. 37. A duplex oligodeoxyribonucleotide, having two complementary strands, and (ii) at least one strand has contiguous nucleoside subunits joined by intersubunit linkages, where at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages as follows: where X is -O ' , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl. 38. A duplex molecule of claim 44, where 50% or greater of the intersubunit linkages of at least one strand are N3'→P5' phosphoramidate linkages. 39. A duplex molecule of claim 44, where all of the intersubunit linkages of at least one strand are N3'→P5' phosphoramidate linkages. 40. A duplex molecule of claim 44, where said complementary strands are connected by a flexible hinge region. 41. A duplex molecule, where each complementary strand has 5' and 3' ends, and said flexible hinge region connects the complementary strand ends in one of the orientations selected from the group consisting of 5' to 3', 3' to 5', 3' to 3', and 5' to 5'. 42. A pharmaceutical composition, comprising an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where (i) at least 2 contiguous subunits are joined by N3'-*P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'-*P5' phosphoramidate intersubunit linkages as follows: where X is -O ~ , -OR or -R, and R is selected from the group consisting of alkyl, alkenyl, aryl, and aralkyl, and (ii) said oligodeoxyribonucleotide has a sequence of nucleoside subunits effective to form a triplex with a target duplex DNA and a pharmaceutically acceptable carrier. 43. A pharmaceutical compositions, comprising an oligodeoxyribonucleotide having contiguous nucleoside subunits joined by intersubunit linkages, where said oligodeoxyribonucleotide, is capable of forming a duplex with RNA and has, where at least 2 contiguous subunits are joined by N3'→P5' phosphoramidate intersubunit, or greater than 3 of the total intersubunit linkages are N3'→P5' phosphoramidate intersubunit linkages as follows: where X is O * , OR or -R, and R is selected from the going consisting of alkyl, alkenyl, aryl, and aralkyl, and said oligodeoxyribonucleotide has a sequence of nucleoside subunits effective to form a duplex structure with an RNA target, and a pharmaceutically acceptable carrier.

Inventors: CHEN JER-KANG, GRYAZNOV SERGEI M, SCHULTZ RONALD G, CHEN, JER-KANG, GRYAZNOV, SERGEI, M., SCHULTZ, RONALD, G.
Applicants: CHEN JER KANG, GRYAZNOV SERGEI M, LYNX THERAPEUTICS INC, SCHULTZ RONALD G, CHEN, JER-KANG, GRYAZNOV, SERGEI, M., LYNX THERAPEUTICS, INC., SCHULTZ, RONALD, G.
Application year: 1995
Main classification: C12Q1
IPC codes: A61K31, A61K48, A61P29, A61P31, A61P35, A61P43, C07H19, C07H21, C12N15, C12Q1, G01N33