INTRACELLULAR ISOFORM OF THE INTERLEUKIN-1 RECEPTOR ANTAGONIST

Abstract: It is described a new interleukin-1 antagonist active both against IL-1a and IL-1B, a new DNA sequence encoding the IL-1 antagonist and the method for obtaining a IL-1 antagonist by the recombinant DNA technique; it is also described the prophylactic, therapeutic and diagnostic use of such new IL-1 antagonist in pathologies deriving from the IL-1 production.

Description: Intracellular isoforπi of the 1nterleuMn-l receptor antagonist FIELD OF THE INVENTION The present invention is in the field of biotechnology. It is described a new interleukin-1 (IL-1) antagonist active both against IL-1 a and IL-IB, a new DNA sequence encoding the LL-1 antagonist and the method for obtaining a LL- 1 antagonist by the recombinant DNA technique. It is also described the prophylactic, therapeutic and diagnostic use of such new IL-1 antagonist in pathologies deriving from the LL-1 production. BACKGROUND OF THE INVENTION There are two distinct genes encoding the interleukin-1 (LL-1) named IL- la and LL-1B, which encode protein LL-1 a and LL-IB respectively. Interleukin LL-1 a and LL-IB are pleiotropic cytokines, which, although their sequences show scarce analogy, exert a variety of similar effects on different tissues and act on many human pathologies, in particular on the immunitary response of the organism and on inflammatory processes. Both the proteins have a molecular weight of about 17.5 KDa and have been previously synthesised as precursor molecule of larger size having a molecular weight of about 31 KDa. LL-ls are potent inflammatory and pyrogenic cytokines that normally have beneficial effects but can also have extremely unhealthy effects for the organism. They can, for example, participate in the pathogenesis of symptoms of the autoimmune pathologies like lupus entematosus and, in particular, they are involved as mediators to provoke damages to tissues as for example in rheumatoid arthritis. Many of the biological effects of LL-1 are similar to those that can be observed during a septic event. Recent studies demonstrated that the endovenous administration of LL-1 in doses from 1 to 10 ng kg gives rise to fever, sleepiness, anorexia, generalised myalgia, arthralgia and cephalea. Since LL-1 have pleiotropic biological activities, many of which influence negatively the organism, the powerful effects of LL-1 should be under strict physiological control. LL-1 synthesis is inhibited by anti-inflammatory cytokines, prostaglandins and glucocorticoids and the existence of multiple levels of inhibition of LL-1 points to the necessity of a strict control of this mediator. LL-1 is the only cytokine for which an antagonist polypeptide for the receptor has been described up to now: the third known component until today of the LL-1 family is the antagonist for the LL-1 receptor (LL-1 ra). All three components (LL-la, LL-IB, LL-lra) recognise and bind to the same receptor on cell surface (IL-IR); LL-la and LL-IB binding to LL-IR transmit a signal, whilst IL-ra does not. There are two types of LL-1 receptors named LL-IRI and LL-1 RE. LL-lra is a polypeptide which binds LL-IRI, and with less affinity LL-IRLJ, without any agonistic activity. LL-lra production is induced in different cellular types, including mononuclear phagocytes, polymorphonuclear cells (PMN) and fibroblasts, by IgG, cytokines and bacterial products. Until now two molecular forms of LL-lra have been identified and cloned: 1) secreted LL-lra (sLL-lra) contains a classical leader sequence of 25 amino acids giving a mature protein of 152 amino acids; 2) intracellular LL-lra (icLL- lra) lacks a leader sequence thus predicting that this protein remains intracellular. sLL-lra and icLL-lra are generated from the same gene. icLL-lra transcripts originate from an alternative starting site and from the splicing of a first alternative exon into an internal splice acceptor site located in the first exon of sLL-lra. The predicted proteins are thus identical except in their NH 2 ends, where the first 21 amino acids of sLL-lra are substituted by four amino acids in icLL-lra. Expression of transcripts encoding sLL-lra and icLL-lra is differently regulated. The biological significance of icLL-lra is still unclear. Considering that LL-1 is involved in pathogenesis of many diseases it is evident the need of having available medicaments useful to limit the unhealthy effects of LL-1. SUMMARY OF THE INVENTION An object of the present invention is to provide an LL-1 antagonist active against both LL-la and LL-IB and against a combination of them. A further object of the present invention is to provide a DNA sequence encoding an LL-1 antagonist and a method for obtaining such new antagonist by the recombinant DNA technique. Another further object of the present invention is to provide the antagonist in substantially purified form in order to be suitable for use in pharmaceutical compositions active in pathologies that require LL-1 inhibition. Further objects and advantages of the invention will be evident in the following description. BRIEF DESCRIPTION OF THE FIGURES AND SEQUENCE LISTING Figure 1 describes the DNA sequence and the sequence of the protein, for the portion not in common, of icLL-lrall (SEQ LD NO:8 and SEQ LD NO:9) compared to those of classic sLL-lra (icLL-lral; SEQ LD NO: 6) and of sLL-lra (SEQ LD NO:4 and SEQ LD NO: 5), and it further describes the DNA sequence and the encoded protein for the portion of LL-lra in common (SEQ LD NO: 13 and SEQ LD NO: 14). Figure 2 describes the RT-PCR analysis of icLL-lrall expression in different cell types. Figure 3 describes the Western blot analysis of recombinant icLL-lrall. Figure 4 describes the effects of icLL-lrall on LL-1 induced expression of E-selectin in endothelial cells. SEQ LD NO: 1 reports the sequence of an oligonucleotide named LRA5 for use in RT-PCR. SEQ LD NO:2 reports the sequence of an oligonucleotide, corresponding to nucleotides 69-70 ofB-actin cDNA, for use in RT-PCR. SEQ ID NO: 3 reports the sequence of a backward oligonucleotide, complementary to nucleotides 430-449, for use in RT-PCR. SEQ LD NO:4 reports the DNA sequence encoding sLL-lra for the portion not in common. SEQ LD NO: 5 reports the amino acid sequence of sLL-lra for the portion not in common. SEQ ID NO: 6 reports the DNA sequence encoding three amino acids of icLL-lral for the portion not in common. SEQ LD NO: 7 reports the three amino acids of icLLl-ral for the portion not in common. SEQ ID NO: 8 reports the DNA sequence encoding icLL-lrall for the portion not in common. SEQ ID NO: 9 reports the amino acid sequence of icLL-lrall for the portion not in common. SEQ LD NO: 10 reports the DNA sequence encoding LL-lra for the portion in common. With regard to questions related to the "Patentin EPO" program, for the preparation of the sequences a G nucleotide was added in the first position of the sequence in order to permit the encode of the first amino acid Glu and further in order to avoid the formation of a stop codon in the inner side of the sequence. SEQ LD NO:l 1 reports the amino acid sequence of LL-lra for the portion in common. SEQ LD NO: 12 reports the sequence of 21 amino acids representing a icLL- 1 raLT fragment not in common with the other LL- Iras. SEQ LD NO: 13 reports the DNA sequence encoding the complete icLL- lraLL SEQ LD NO: 14 reports the amino acid sequence of complete icLL-1 raLT. DESCRIPTION OF THE INVENTION This new LL-1 antagonist was generated by inserting in the frame of the DNA encoding icLL-lra a new 63 base pairs (bp) sequence between the first icLL-lra specific exon and the internal acceptor site of the first exon of sLL- Ira. By RT-PCR experiments the present inventors found that this new transcript is expressed in activated monocytes and fibroblasts and in polymorphonuclear cells (PMN). Expression in COS cells revealed that this new antagonist is mostly intracellular and has a molecular weight (MW) of approximately 25 KDa in SDS-PAGE. The new recombinant antagonist shows LL-1 inhibitory activity. In the present application, for reason of clearness and easiness, the presently known icLL-lra are indicated as icLL-lra type I (icLL-lral), whereas the new antagonist here described and object of the present invention is defined as icLL-lra type II (icLL-lrall). Examples of pathologies in which the new antagonist according to the invention can be advantageously used for prophylactic, therapeutic or diagnostic use are rheumatoid arthritis, septic shock, acute myelomonocytic leukaemia, immunological reaction of transplantation against host, acquired immunodeficiency syndrome (AIDS), ulcerative colitis and all autoimmune diseases in general. An embodiment of the invention is the administration of a pharmacological active amount of icLL-lrall to people having a high risk to develop pathologies requiring LL-1 inhibition or to people already showing pathologies like sepsis. An example of the category above cited are patients waiting for a surgical operation. Any route of administration compatible with the active principle can be used, but particularly preferred is the parenteral administration because it permits to have, in short times, systemic effects. For this reason, it is preferable the administration of a endovenous bolus just before, during or after the surgical operation. The dose of icIL- lraU to be administered depends on the basis of the medical prescriptions according to age, weight and the individual response of the patient. The dosage can be between 0.05 and 30 mg/Kg body weight and the preferable dose is between 0.1 and 10 mg Kg body weight. The pharmaceutical composition for parenteral use can be prepared in injectable form comprising the active principle and a suitable vehicle. Vehicles for the parenteral administration are well known in the art and comprise, for example, water, saline solution, Ringer solution and dextrose. The vehicle can contain smaller amounts of excipients in order to maintain the solution stability and isotonicity. The preparation of the cited solutions can be carried out according to the ordinary modalities and preferably the icLL-lraH content will be comprised between 1 mg/ml and 10 mg/ml. Further examples of pathologies wherein the new antagonist according to the invention can be advantageously used for prophylactic, therapeutic diagnostic purpose are rheumatoid arthritis, septic shock, acute myelomonocytic leukaemia, immunological reaction of transplantation against host, acquired immunodeficiency syndrome (AIDS), ulcerative colitis and all autoimmune diseases in general. The present invention has been described with reference to the specific embodiments, but the content of the description comprises all modifications and substitutions which can be brought by a person skilled in the art without extending beyond the meaning and purpose of the claims. In the following part some methods for obtaining the invention will be described, although equivalent materials and methods can be used. The following examples are therefore purely illustrative and non-limiting of the invention. EXAMPLE 1 Cloning and characterisation of icIL-lrall MATERIALS AND METHODS Reagents The following commercially available reagents were used for culture and separation of cells: pyrogenfree saline and distilled water for clinical use; RPMI 1640 medium; DMEM medium; Ml 99 medium; L-glutamine; Percoll; Ficoll-Hipaque; aseptically collected fetal calf serum; endothelial cells growth supplement (ECGS), prepared from bovine brain; Heparin. All reagents contained less than 0.125 EU/ml of endotoxin as checked by thr Limulus amebocyte lysate assay. Cells Human circulating PMN and monocytes were separated from the peripheral blood of healthy donors by centrifugation on a discontinuous (46% for monocytes and 62% for PMN) gradient of isoosmotic (285 mOsm) Percoll, as described in Colotta F., Peri G., Villa SA., Mantovani A., Rapid killing of actinomycin D treated tumour cells by human mononuclear cells. J. Immunol. 132:936, 1984. Cells were recovered at the interface, washed twice in saline and resuspόnded in the medium. PMN and monocytes recovery was higher than 90% and purity higher than 98%, as assessed by morphological examination of stained cytocentrifuged cells. The cell culture medium routinely used for PMN and monocytes was RPMI 1640 with 2 mM L-glutamine and 10% FCS. Human endothelial cells (EC) were obtained from umbilical veins and cultured, as described in detail in the literature (Allavena P., Paganin C, Martin-Padura I., Peri G., Gaboli M., Dejana E., Marchisio P.C., Mantovani A, Molecules and structures involved in the adhesion of natural killer cells to vascular endothelium, J. Exp. Med., 173:439, 1991). Confluent cells at 2nd-5th passage maintained in Ml 99 medium with 10% FCS supplemented with ECGS (50 μg ml) and Heparin (100 μg/ml) were routinely used. COS cells were cultivated in DMEM medium with 10% FCS and 8387 fibroblast cells in RPMI 1640 medium with 10% FCS. After the appropriate treatment, cells were examined for LL-lra mRNA or LL-lra protein as described below. RT-PCR Total RNA was extracted by the guanidinium isothiocianate method with minor modifications. RT-PCR was performed as described in Colotta F., Polentarutti N, Sironi M., Mantovani A., J. Biol. Chem., 267:18278, 1992. Briefly, 1 μg total RNA was reverse transcribed in reverse transcriptase buffer (5 mM MgCl 2 , 50 mM KC1, 10 mM Tris-HCl; pH 8.3) with 2.5 mM random hexamers, 1 mM each deoxynucleotide triphosphate, 1 unit/ml RNase inhibitor, and 2.5 units/ml moloney murine leukaemia virus transcriptase (Perkin Elmer Cetus, Norwalk, CT). Samples were incubated for 10 min at 25°C and then at 42°C for 45 min. Then, cDNA reaction was added with a specific pair of primers designed to amplify cDNAs encoding icLL-lral or icLL-lrall and, as an internal control, human B-actin. Amplification was carried out in 2 mM MgCl , 50 mM KC1, 0.2 M each deoxynucleotide triphosphate, 2.5 units/100 ml Taq polymerase (Perkin Elmer Cetus) and 4 mg/ml of each specific primer (see below). Amplification (30 cycles) was carried out in an automated thermal cycler (Perkin Elmer Cetus) at 95°C, at 55°C and at 72°C for 1.5 min each. Amplified products were run through a 1% ethidium bromide-stained agarose gel along with molecular weight standards (Boehringer Mannheim, Mannheim, Germany). Oligonucleotides were synthesised by the phosphoramidite method. The sequences of oligonucleotides used to selectively amplify icLL-lra were identical to those described in Haskill S. et al., Nail. Acad., USA, 88:3681, 1991. In particular, the authors used oligonucleotides GM397 (indicated here as ERA 1) and GM368 (LRA 4). For icLL-lraLJ amplification the authors used LRA 4 and IRA 5 (SEQ LD NO: l), which specifically recognises the extra exon described here included in the icLL-1 raLT sequence. For B-actin amplification the forward oligonucleotide is reported in SEQ ED NO:2, corresponding to nucleotides 60-79 of B-actin cDNA. The backward oligonucleotide is reported in SEQ ED NO: 3, complementary to nucleotides 430-449. Amplification products were subcloned (TA Cloning System, Invitrogen, San Diego, CA) and sequenced by the dideoxi chain termination method. Expression of icIL-lra products in COS cells The cDNAs containing 32 bp of the 5'-untraslated region, the complete open reading frame and 6 bp (including the stop codon) of the 3'-untraslated region of both the icLL-lral and icLL-lraLT were obtained by RT-PCR with oligonucleotides ERA 4 and ERA 5 as detailed above and then ligated back into the pSF5 expression vector. Fidelity of reverse transcription and amplification was verified by sequencing. The plasmids containing the cDNA in the correct orientation were purified on CsCl gradient and then transfected into COS cells by the calcium precipitate method as described in Sambrook J. et al., Cold Spring Harbor Laboratory Press, 1989. After two days, culture supernatants and sonicated cell lysates examined by ELISA or immunoblotting as detailed below. An empty plasmid (not transfected) was used as a control. Identification of immunoreactive IL-lra A commercial ELISA test (Amersham, Buckinamshire, UK) that identifies both sIL-lra and icLL-lra was used. For the Western blot analysis polyclonal antisera of two rabbits and of one goat were used. COS cells lysates samples and supernatants were run on 12.5% SDS- PAGE electrophoresis and then blotted onto a nitro-cellulose filter (Stratagene, La Jolla, CA, USA). Incubation with primary and secondary antibodies was carried out according to standard protocols. The primary antibody was an anti-LL-lra rabbit polyclonal antibody. The secondary antibody was a goat anti-rabbit immunoglobulin fraction linked to horseradish peroxidase (Amersham). Immunoreactive protein fraction bands were revealed by a chemiluminescence-based procedure (ECL Detection, Amersham) according to manufacturer's instructions. II- 1 -induced expression of E-selectin on EC Confluent EC cultivated in 96 well plates (Falcon) were incubated for 30 minutes with an amount of transfected COS cells lysate (see above) corresponding to 25 to 100 ng of recombinant LL-lra (either icLL-lral or icLL- lraLT) as assessed by a specific ELISA assay (Amersham). As a control, an equal amount of COS lysate obtained from mock transfected cells was used in parallel. Next, EC were exposed for 6 hours to -9- 0.1-1 ng ml human recombinant LL-IB. The detection of E-selectin expression was made with an ELISA assay on adherent EC with the anti-E-selectin monoclonal antibody BB1G-E2 as primary antibody and a rabbit anti-mouse Ig antiserum conjugated with horseradish peroxidase as a secondary antibody. O.D. of the samples was determined by detecting the plates with a spectrophotometer (Flow) at 405 wavelength. RESULTS Identification of icIL-lrall Specific oligonucleotide primers were designed (indicated as ERA 1 and ERA 4 in Fig.1) in order to obtain the whole coding sequence of icLL-lra (Fig.1) by RT-PCR. Amplified products from human PMN were subcloned and sequenced. In addition to the previously known sequence of icLL-lra, the inventor isolated a number of clones whose sequences were identical to the published icLL-lra coding sequence, with the notable exception of an extra sequence of 63 bp between nucleotides 132 and 133 of the icLL-lra sequence. Given the described exon-intron boundaries of icLL- Ira, the extra sequence is inserted between the first leader-less exon of icLL-lra and the internal acceptor site of the first exon of sLL- 1 ra (Fig.1 ). The predicted amino acid sequence is shown in Fig.l. The new protein (thereafter referred to as icLL-lra type II) has the first three amino acids at the NH 2 terminus in common with the classical icLL-lra (icLL-lra type I), followed by a new sequence of 21 amino acids. The rest of the two proteins is identical. Curiously, the junction with the internal acceptor site of the first exon of sLL-lra always generated, both for sLL-lra and icLL-lral and for icLL-lraLT, the same amino acid residue, i.e. glutamic acid (Fig.l). The most striking characteristic of the inserted amino acid extra sequence is the presence of seven glycin residues, six of which are consecutive. Glycin residues are flanked on both sides by glutamic acid residues. icEL-lraLT consists of 180 amino acids. The overall hydrophilic pattern of icLL-lraLT is similar to that of icLL-lral, still lacking an hydrophobic leader peptide at the NH terminus. Expression of icIL-rall To identify icLL-lraLT transcripts, RT-PCR analysis was performed with a pair of specifically designed oligonucleotides (ERA 5 and ERA 4, Fig.l), with an expected amplified product of 33 bp. As shown in Fig.2, transcripts encoding icLL-lraLT were detectable in PMA-, LL-1- and TNF-activated fibroblasts. A faint but detectable band was evident in LPS-treated monocytes. Also PMN, either untreated or activated (Fig.2) showed a very faint band of the expected size. The specificity of amplified products indicated in Fig.2 was confirmed by subcloning and sequencing. Expression of recombinant icIL-lrall COS cells were transfected with the DNA sequence encoding icLL-lraLT and, by way of comparison, with that encoding icIL-lral. Next, cell lysates and supernatants were examined by Western blot. The polyclonal antisera used in these experiments recognised equally well icLL-lraLT and icLL-lral (Fig.3). Most, if not all, of icLL-lraLT and icLL-lral were found in cell lysates. Recombinant icLL-lral migrated as a predominant band of 22 KDa, whereas icEL-lraEt showed a mass of approximately 25 KDa. Inhibition of IL-IB activity by recombinant icIL-lrall Recombinant icLL-lraLT was examined for LL-1 inhibiting activity. To this aim the authors chose the EL-l -induced expression of E-selectin on endothelial cells, because this assay is sensitive (detectable induction at 100 pg/ml LL-1, or less) and rapid (6 hours incubation with LL-1). Lysates of mock transfected COS cells did not significatively reduce the LL-1 activity. icLL-lraLT had no agonistic activity. As shown in Fig.4, recombinant icEL-lraLT inhibited in a dose- dependent fashion LL-1 activity. These data provide evidence that icLL-1 raLT is indeed an inhibitor of IL- 1. DISCUSSION The inventors describe a new molecular form of icLL-lra. The new molecule is generated by insertion of 63 bp between the first leader-less exon of icLL-lra and the internal acceptor site of the first exon of sLL-lra. Since the resulting protein is partially identical to classical icLL-lra, with the exception of an extra sequence of 21 amino acids located in the NH 2 terminus of the molucule, the inventors suggest to term this new form as LL- lra type LI, referring to the classical icLL-lra sequence as icLL-lra type I. RT-PCR experiments demonstrated that icLL-lraLT transcripts are inducible in monocytes and fibroblasts. Recombinant icLL-lraLT expressed in COS cells had an apparent MW of approximately 25 KDa and an inhibitor activity of LL-1 comparable to that exerted by icLL-lral expressed under the same experimental conditions. Transcripts coding for icEL- Ira and sEL- Ira are generated from the same gene by means of usage of differential splicing. icLL-lra is generated by an alternative start of transcription of an exon inserted into an internal acceptor site of the first exon containing the leader sequence of sLL-lra. The results obtained by the inventors suggest a new organisation of LL-lra gene, in which an extra exon is located between the first exon of, respectively, classical icLL-lra and sLL-lra. Use of this new exon generates a polypeptide molecule which, still lacking a signal peptide, differs from icLL-lral at its N terminus by the insertion of 21 amino acids, still remaining inhibitory capacity against LL-1. Use of alternative splicing to generate different LL-lra molecules appears to be highly regulated. icLL-lraLT transcripts were induced by LL-1 , TNF and phorbol esters in fibroblasts and by LPS in monocytes. In fibroblasts, phorbol esters were found to selectively induce icIL-lra transcripts, whereas LL- 1 and TNF induced both sLL-lra and icLL-lra mRNAs. In monocytes, LL-13, which augmented both transcripts of sLL-lra and icLL-lral, failed to induce icLL- lrafl. Finally, PMN, in which sLL-lra and icLL-lra are costitutively expressed and inducible, expressed very few transcripts, as pointed out by RT-PCR. Overall, these data indicate that the mechanisms inducing the differential splicing generating the three forms of EL- Ira are differentially regulated in response to external signals. The amino acid sequence of the extra sequence described here is surprising in that it contains seven residues of glycin, six of which are consecutive. Glycin-rich sequences are present in molecules with different biological activities, including the atrial natriuretic clearance receptor, the HOX11 home box gene, the intermediate filaments keratins and nuclear proteins involved in centromere binding or RNA splicing. Apart from glycin residues, however, no obvious homology was evident between these proteins and icLL-lrall in the amino acid sequence flanking glycin-rich regions. EL-l system shows an extraordinary level of complexity, consisting of two agonists, two receptors, one of which is an inhibitor of EL-l, and a receptor antagonist, for which at least three different molecular forms could exist taking into account the results obtained. Although the biological significance of the intracellular forms of EL- Ira remains to be clearly established, the data here reported indicate that by alternative splicing two different forms of icLL-lra can be generated in response to selected external stimuli, with different N termini. The existence of multiple and comrlex levels of control of EL-l points to the absolute requirement for a tight physiological control of the inflammatory potential of this cytokine. DESCRIPTION OF FIGURES Figure 1 DNA sequence and predicted protein sequence of icIL-lrall compared to classical icIL-lra(icIL-lral) and sIL-lra. The upper part of Figure 1 shows DNA and protein sequences specifically represented in sLL-lra, icLL-lral and icLL-lraLT. The lower part of Figure 1 shows the sequence in common among the three forms of LL-lra. The entire sequences for each molecule are thus generated by the junction of each specific portion with the common sequence. For clarity, the DNA sequence of icLL-lra starts from nucleotide 91 of the published 5' untraslated sequence, and only 6 bp of the 3' untraslated sequence are reported. The common EL- Ira sequence starts with the internal acceptor site located in the first exon of sLL-lra, corresponding to nucleotide 133 of the complete icLL-lral sequence and to nucleotide 88 of the complete sLL- Ira sequence. Arrows indicate forward (ERA 1 and ERA 5) and backward (ERA 4) oligonucleotides used for RT-PCR analysis, as described in the text. The oligonucleotide ERA 5 recognises only icEL-lraLT DNA. Figure 2 RT-PCR analysis of icIL-lrall expression in different cell types RNAs from 8387 fibroblasts (panel A), monocytes (B) and PMN (C) were reverse-transcribed. Each DNA synthesis reaction was then divided in two samples, one of which amplified with oligonucleotides ERA 5 (forward) and ERA 4 (backward) for detection of icLL-lraLT transcripts, and the other amplified with B-actin specific oligonucleotides (see Material and Methods Section). Amplified products were then examined through an ethidium bromide- stained agarose gel. Amplified products corresponding to B-actin are reported on the left side of the standard and the amplified products corresponding to icLL-lraLT (on the right) are indicated by a arrow. The specificity of these bands was confirmed by subcloning and sequencing. Figure 3 Western blot analysis of recombinant icIL-lrall Cell lysates from COS cells transfected with DNAs encoding icLL-lral (2) or icLL-lrall (3) or with an empty vector which does not contain such DNA (1) were examined by immunoblotting with an anti-LL-lra rabbit polyclonal antibody. Molecular weight standards are indicated. Figure 4 Effects of icIL-lrall on IL-1-induced expression of E-selectin on endothelial cells Endothelial cells were treated with 0.1 or 1 ng/ml of human LL-IB, with or without 25-100 ng/ml of icLL-1 raLT or equivalent amounts of COS cell lysates obtained from cells which were mock transfected by means of an empty vector, as explained in details in the Material and Method section. After 6 hours of incubation, the endothelial cells were examined for E- selectin expression by an ELISA test performed on adherent cells. The data reported are percentages of LL-1 -induced E-selectin expression for the control. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: (A) NAME: APPLIED RESEARCH SYSTEMS ARS HOLDING N.V. (B) STREET: 14 JOHN B. GORSIRAWEG (C) CITY: CURACAO (E) COUNTRY: NETHERLANDS ANTILLES (F) POSTAL CODE (ZIP) : NONE (G) TELEPHONE: 599-9639300 (H) TELEFAX: 599-9614129 (ii) TITLE OF INVENTION: INTERLEUKIN-1 ANTAGONIST (iii) NUMBER OF SEQUENCES: 14 (iv) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.30 (EPO) (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 25 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION:1..25 (D)OTHER INFORMATIO :/note= "RT-PCR oligonucleotide named IRA5" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: CTGACTTGTA TGAAGAAGGA GGTGG 25 (2) INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION:1..20 (D) OTHER INFORMATION:/note= "RT-PCR oligonucleotide corresponding to 60-79 of B-actin" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: GCGCTCGTCG TCGACAACGG 20 (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATIO :1..21 (D) OTHER INFORMATION:/note= "RT-PCR backward oligonucleotide complementary to 430-449" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: GATAGACAAC GTACATGGCT G 21 (2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 87 base pairs - 16- (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:24..86 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION:1..87 (D) OTHER INFORMATION:/note= "Sequence of sIL-lra not in common" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: GAATTCCGGG CTGCAGTCAC AGA ATG GAA ATC TGC AGA GGC CTC CGC AGT 50 Met Glu lie Cys Arg Gly Leu Arg Ser 1 5 CAC CTA ATC ACT CTC CTC CTC TTC CTG TTC CAT TCA G 87 His Leu lie Thr Leu Leu Leu Phe Leu Phe His Ser 10 15 20 (2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: Met Glu lie Cys Arg Gly Leu Arg Ser His Leu lie Thr Leu Leu Leu 1 5 10 15 Phe Leu Phe His Ser 20 (2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 42 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:33..41 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION:1..42 (D) OTHER INFORMATION: /note= "Sequence of intracellular IL-lra type I not in common" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: CAGAAGACCT CCTGTCCTAT GAGGCCCTCC CC ATG GCT TTA G 42 Met Ala Leu 1 (2) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: Met Ala Leu 1 (2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 105 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 33..104 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..105 (D) OTHER INFORMATION:/note= "Sequence of intracellular IL-lra type II not in common" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: CAGAAGACCT CCTGTCCTAT GAGGCCCTCC CC ATG GCT TTA GCT GAC TTG TAT 53 Met Ala Leu Ala Asp Leu Tyr 1 5 GAA GAA GGA GGT GGA GGA GGA GGA GAA GGT GAA GAC AAT GCT GAC TCA 101 Glu Glu Gly Gly Gly Gly Gly Gly Glu Gly Glu Asp Asn Ala Asp Ser 10 15 20 AAG G 105 Lys (2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 24 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: Met Ala Leu Ala Asp Leu Tyr Glu Glu Gly Gly Gly Gly Gly Gly Glu 1 5 10 15 Gly Glu Asp Asn Ala Asp Ser Lys 20 (2) INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 474 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: CDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION:1..468 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION:1..474 (D) OTHER INFORMATION:/note= "Common IL-lra seq.; a G was added in the first position for software reason, so as the first codon codes for Glu and so as the creation of a stop codon in the inner region of the seq. is avoided" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: GAG ACG ATC TGC CGA CCC TCT GGG AGA AAA TCC AGC AAG ATG CAA GCC 48 Glu Thr lie Cys Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gin Ala 1 5 ' 10 15 TTC AGA ATC TGG GAT GTT AAC CAG AAG ACC TTC TAT CTG AGG AAC AAC 96 Phe Arg lie Trp Asp Val Asn Gin Lys Thr Phe Tyr Leu Arg Asn Asn 20 25 30 CAA CTA GTT GCT GGA TAC TTG CAA GGA CCA AAT GTC AAT TTA GAA GAA 144 Gin Leu Val Ala Gly Tyr Leu Gin Gly Pro Asn Val Asn Leu Glu Glu 35 40 45 AAG ATA GAT GTG GTA CCC ATT GAG CCT CAT GCT CTG TTC TTG GGA ATC 192 Lys lie Asp Val Val Pro lie Glu Pro His Ala Leu Phe Leu Gly lie 50 55 60 CAT GGA GGG AAG ATG TGC CTG TCC TGT GTC AAG TCT GGT GAT GAG ACC 240 His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr 65 70 75 80 AGA CTC CAG CTG GAG GCA GTT AAC ATC ACT GAC CTG AGC GAG AAC AGA 288 Arg Leu Gin Leu Glu Ala Val Asn lie Thr Asp Leu Ser Glu Asn Arg 85 90 95 AAG CAG GAC AAG CGC TTC GCC TTC ATC CGC TCA GAC AGT GGC CCC ACC 336 Lys Gin Asp Lys Arg Phe Ala Phe lie Arg Ser Asp Ser Gly Pro Thr 100 105 110 ACC AGT TTT GAG TCT GCC GCC TGC CCC GGT TGG TTC CTC TGC ACA GCG 384 Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala 115 120 125 ATG GAA GCT GAC CAG CCC GTC AGC CTC ACC AAT ATG CCT GAC GAA GGC 432 Met Glu Ala Asp Gin Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly 130 135 140 GTC ATG GTC ACC AAA TTC TAC TTC CAG GAG GAC GAG TAGTAC 474 Val Met Val Thr Lys Phe Tyr Phe Gin Glu Asp Glu 145 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 156 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: Glu Thr lie Cys Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gin Ala 1 5 10 15 Phe Arg lie Trp Asp Val Asn Gin Lys Thr Phe Tyr Leu Arg Asn Asn 20 25 30 Gin Leu Val Ala Gly Tyr Leu Gin Gly Pro Asn Val Asn Leu Glu Glu 35 40 45 Lys lie Asp Val Val Pro lie Glu Pro His Ala Leu Phe Leu Gly lie 50 55 60 His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr 65 70 75 80 Arg Leu Gin Leu Glu Ala Val Asn lie Thr Asp Leu Ser Glu Asn Arg 85 90 95 Lys Gin Asp Lys Arg Phe Ala Phe lie Arg Ser Asp Ser Gly Pro Thr 100 105 110 Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala 115 120 125 Met Glu Ala Asp Gin Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly 130 135 140 Val Met Val Thr Lys Phe Tyr Phe Gin Glu Asp Glu 145 150 155 (2) INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (v) FRAGMENT TYPE: N-terminal (ix) FEATURE: (A) NAME/KEY: Peptide (B) LOCATION:1..21 (D) OTHER INFORMATION:/note= "A portion of the intracellular IL-lra type II not in common" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: Ala Asp Leu Tyr Glu Glu Gly Gly Gly Gly Gly Gly Gly Gly Glu Asp 1 5 10 15 Asn Ala Asp Ser Lys 20 (2) INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 579 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 34..573 (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION:1..579 (D) OTHER INFORMATION:/note= "Intracellular IL-lra type II" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: CAGAAGGACC TCCTGTCCTA TGAGGCCCTC CCC ATG GCT TTA GCT GAC TTG TAT 54 Met Ala Leu Ala Asp Leu Tyr 1 5 GAA GAA GGA GGT GGA GGA GGA GGA GAA GGT GAA GAC AAT GCT GAC TCA 102 Glu Glu Gly Gly Gly Gly Gly Gly Glu Gly Glu Asp Asn Ala Asp Ser 10 15 20 AAG GAG ACG ATC TGC CGA CCC TCT GGG AGA AAA TCC AGC AAG ATG CAA 150 Lys Glu Thr lie Cys Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gin 25 30 35 GCC TTC AGA ATC TGG GAT GTT AAC CAG AAG ACC TTC TAT CTG AGG AAC 198 Ala Phe Arg lie Trp Asp Val Asn Gin Lys Thr Phe Tyr Leu Arg Asn 40 45 50 55 AAC CAA CTA GTT GCT GGA TAC TTG CAA GGA CCA AAT GTC AAT TTA GAA 246 Asn Gin Leu Val Ala Gly Tyr Leu Gin Gly Pro Asn Val Asn Leu Glu 60 65 70 GAA AAG ATA GAT GTG GTA CCC ATT GAG CCT CAT GCT CTG TTC TTG GGA 294 Glu Lys lie Asp Val Val Pro lie Glu Pro His Ala Leu Phe Leu Gly 75 80 85 ATC CAT GGA GGG AAG ATG TGC CTG TCC TGT GTC AAG TCT GGT GAT GAG 342 lie His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu 90 95 100 ACC AGA CTC CAG CTG GAG GCA GTT AAC ATC ACT GAC CTG AGC GAG AAC 390 Thr Arg Leu Gin Leu Glu Ala Val Asn lie Thr Asp Leu Ser Glu Asn 105 110 115 AGA AAG CAG GAC AAG CGC TTC GCC TTC ATC CGC TCA GAC AGT GGC CCC 438 Arg Lys Gin Asp Lys Arg Phe Ala Phe lie Arg Ser Asp Ser Gly Pro 120 125 130 135 ACC ACC AGT TTT GAG TCT GCC GCC TGC CCC GGT TGG TTC CTC TGC ACA 486 Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr 140 145 150 GCG ATG GAA GCT GAC CAG CCC GTC AGC CTC ACC AAT ATG CCT GAC GAA 534 Ala Met Glu Ala Asp Gin Pro Val Ser Leu Thr Asn Met Pro Asp Glu 155 160 165 GGC GTC ATG GTC ACC AAA TTC TAC TTC CAG GAG GAC GAG TAGTAC 579 Gly Val Met Val Thr Lys Phe Tyr Phe Gin Glu Asp Glu 170 175 180 (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 180 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: Met Ala Leu Ala Asp Leu Tyr Glu Glu Gly Gly Gly Gly Gly Gly Glu 1 5 10 15 Gly Glu Asp Asn Ala Asp Ser Lys Glu Thr lie Cys Arg Pro Ser Gly 20 25 30 Arg Lys Ser Ser Lys Met Gin Ala Phe Arg lie Trp Asp Val Asn Gin 35 40 45 Lys Thr Phe Tyr Leu Arg Asn Asn Gin Leu Val Ala Gly Tyr Leu Gin 50 55 60 Gly Pro Asn Val Asn Leu Glu Glu Lys lie Asp Val Val Pro lie Glu 65 70 75 80 Pro His Ala Leu Phe Leu Gly lie His Gly Gly Lys Met Cys Leu Ser 85 90 95 Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gin Leu Glu Ala Val Asn 100 105 110 lie Thr Asp Leu Ser Glu Asn Arg Lys Gin Asp Lys Arg Phe Ala Phe 115 120 125 lie Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys 130 135 140 Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp Gin Pro Val Ser 145 150 155 160 Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe 165 170 175 Gin Glu Asp Glu 180

Claims: CLAEMS 1. Purified protein having antagonist activity against at least one of the substances selected from the group consisting of interleuldn a and interleuldn B, or fragments thereof, characterised by the fact of comprising the amino acid sequence reported in SEQ ED NO: 12. 2. A purified protein having antagonist activity against at least one of the substances selected from the group consisting of interleuldn a and interleuldn B, or fragments thereof, characterised by the fact to have the amino acid sequence reported in SEQ ID NO: 14. 3. A purified protein according to claim 2, characterised by the fact of being obtained by recombinant DNA technique. 4. Isolated DNA sequence encoding a EL-l antagonist having the amino acid sequence according to claim 2. 5. Isolated DNA sequence according to claim 4, characterised by the fact of having reported in SEQ ED NO: 13. 6. Isolated DNA sequence that ibridizes with the probe having the sequence reported in SEQ ED NO: 1. 7. Vector comprising the DNA sequence encoding the protein according to claim 2. 8. Cell culture transfected with DNA encoding the protein according to claim 2. 9. Cell culture according to claim 2, characterised by the fact of being obtained by mammalian cells. 10. Process for obtaining a LL-1 antagonist by the recombinant DNA technique comprising: a) cultivating host cells as described in claims 8 and 9 containing a DNA sequence able to produce the protein according to claim 2; b) collecting and isolating the encoded protein. 11. Process of obtaining a LL-1 antagonist by recombinant DNA technique, characterised by the fact that the DNA sequence is that reported in SEQ ED NO: 13. 12. Use of a purified protein having the protein according to claim 2, for medical use. 13. Use of a purified protein having the sequence according to claim 2 for the preparation of pharmaceutical compositions active in pathologies requiring LL-1 inhibition. 14. Use according to claim 13, characterised by the fact that the pathology is selected from the group of autoimmune pathologies. 15. Use according to claim 14, characterised by the fact that the pathology is selected from the group consisting of rheumatoid arthritis, septic shock, acute myelomonocytic leukaemia, immunological reaction of transplantation against host, acquired immunodeficiency syndrome (AEDS), ulcerative colitis.

Inventors: COLOTTA FRANCESCO, MANTOVANI ALBERTO, MUZIO MARTA, COLOTTA, FRANCESCO, MANTOVANI, ALBERTO, MUZIO, MARTA
Applicants: APPLIED RESEARCH SYSTEMS, APPLIED RESEARCH SYSTEMS ARS HOLDING N.V.
Application year: 1995
Main classification: C12N15
IPC codes: A61K38, A61P7, A61P29, A61P31, A61P35, A61P37, C07K5, C07K14, C12N5, C12N15