3 AND 5 ALKYL AND PHENYL 4-(HYDROXY OR ACYLOXY)-ALKYL SUBSTITUTED 2(5H)-FURANONES AS ANTI-INFLAMMATORY AGENTS

Abstract: Novel anti-inflammatory furanone compounds have formula (1), where R1 independently is H, phenyl, C1-C6 alkyl substituted phenyl, halogen substituted phenyl, or alkyl of 1 to 9 carbons and n is an integer having the values of 1 or 2, and where when n is 1, the R1 group is attached either to the 3 or to the 5 position of the 2-furanone, when n is 2, then R1 is attached to both the 3 and 5 positions, with the proviso that when n is 1, then R1 is not H; Y1 is H, alkyl of 1 to 20 carbons, phenyl C1-C20 alkyl, C1-C20 alkenyl containing one or more olefinic bonds, PO(OH)2, PO(OH)OR2, PO(OH)R2, PO(OR2)2, where R2 is independently alkyl of 1 to 20 carbons, phenyl, halogen substituted phenyl or C1-C6 alkyl substituted phenyl, further Y1 is CO-R3, CO-OR3, CONHR3, SO2R3, SO2NHR3, (CH2)p-O-R3, or (CH2)p-O-(CH2)m-O-R3, where p and m are integers and are independently 1 to 20 and R3 is H, C1-C20 alkyl, C1-C20 alkenyl containing one or more olefinic bonds, phenyl, halogen substituted phenyl or C1-C6 alkyl substituted phenyl, with the proviso that when Y1 is CO-OR3 or CONHR3, then R3 is not hydrogen; Y2 is H, an alkyl group of 1 to 25 carbons, phenyl, naphthyl, phenyl (C1-C20)alkyl-, naphthyl (C1-C20)alkyl-, halogen substituted phenyl, C1-C6 alkyl substituted phenyl, halogen substituted naphthyl, C1-C6 substituted naphthyl.

Description: 3 AND 5 ALKYL AND PHENYL 4-(HYDROXY OR ACYLOXY)-ALKYL SUBSTITUTED 2 (5H)-FURANONES AS ANTI-INFLAMMATORY AGENTS BACKGROUND OF THE INVENTION 1- Field of the Invention The present invention is directed to novel 2- and 5- alkyl and phenyl substituted 4-(1-hydroxy-, 1- acyloxy-, or 1-carbamoyloxy) -2 (5H)-furanones which compounds are active as anti-inflammatory agents. The present invention is also directed to pharmaceutical compositions which comprise one or more of the novel compounds of the invention, to the methods of using these pharmaceutical compositions, and to the chemical processes of making the novel compounds. 2. Brief Description of the Prior Art Manoalide is a compound isolated from a marine sponge [E. D. de Silva et al., Tetrahedron Letters 21:1611-1614 (1980)] which has anti-inflammatory, immunosuppressive and analgesic properties. Manoalide the structure of which is shown below, includes a 5- hydroxy-2 (5H) -furanone moiety, attached in the 4- position of the furanone ring to the rest of the molecule. Certain analogs of manoalide, such as seco- manoalide and dehydro-seco-manoalide also have anti- inflammatory activity. For further description of the biological activity of manoalide and some of its derivatives reference is made to United States Patent Nos. 4,447,445, 4,786,651, 4,789,749 and to European Patent Application No. 0 133 376 (published on February 20, 1985) . Synthetic analogs of manoalide, particularly analogs having various substituents on the furanone moiety of manoalide, are described in several United States patents and applications for United States patent assigned to the same assignee as the present application. Among these, United States Patent No. 5,045,564 discloses 4-substituted 2-5 (H) -furanones as anti-inflammatory and calcium channel antagonist agents. United States Patent No. 5,183,906 (issued on February 2, 1993) 3 and 5 alkyl and phenyl 4-(l- hydroxy, 1-acyloxy or 1-carbamoyloxy) -5-hydroxy-2- furanones, as anti-inflammatory and calcium channel antagonist agents. U.S. Patent No. 5,268,387 describes a method for treating an imbalance between bone production and resorption with furanone compounds, the general examples of which include 3 and 5 alkyl and phenyl 4-(l-hydroxy, 1-acyloxy or 1-carbamoyloxy) -2-furanones, and the specific examples of which include 3 and 5 alkyl and phenyl 4- ( 1-hydroxy , l-acyloxy or 1- carbamoyloxy) -5-hydroxy-2-furanones. European Patent Application No. 0 534 907 Al (published March 31, 93) discloses 3,5-dialkyl, 3,5,- dialkyl-4-hydroxy, and 3,4, 5-trialkyl-2-furanones as agents for treatment of autoimmune diseases. As further general background Published European Patent Application No. 0 295 056 is noted, which discloses 4-substituted 5-hydroxy-2 (5H) -furanones having anti-inflammatory, immunosuppressive and anti- proliferative activity where the substituents in the 4 position are a variety 1-hydroxyalkyl, 1-acyloxy-alkyl and 1-carbamoyloxy-alkyl groups. United States Patent No. 4,855,320 discloses 5- arylalkyl-4-alkoxy-2 (5H) -furanones as anti-convulsive and anti-epileptic agents. Published European Patent Application No. 0 209 274 discloses 4-alkyl-5-hydroxy-2 (5H) -furanones as anti-inflammatory and anti-allergy agents. Chemical Abstracts Volume 107 236559t (1987) discloses 4-acyloxy 5-hydroxy-2 (5H)-furanones. 2-Substituted 4-furaldehydes (5-substituted 3- furaldehydes) are described in United States Patent No. 4,935,530. SUMMARY OF THE INVENTION The present invention covers compounds of Formula Formula 1 where χ independently is H, phenyl, C- j^ -Cg alkyl substituted phenyl, halogen substituted phenyl, or alkyl of 1 to 9 carbons and n is an integer having the values of 1 or 2, and where when n is i the R^ group is attached either to the 3 or to the 5 position of the 2- furanone, when n is 2 then R^ is attached to both the 3 and 5 positions, with the proviso that the substituents in the 3 and 5 positions both cannot be hydrogen;; Y-L is H, alkyl of 1 to 20 carbons, phenyl C^C^ alkyl, C-_ - C 20 alkenyl containing one or more olefinic bonds, PO(OH) 2 , PO(OH)OR 2 , PO(OH)R 2 , PO(OR 2 ) 2 , where R 2 is independently alkyl of 1 to 20 carbons, phenyl, halogen substituted phenyl or C-^-Cg alkyl substituted phenyl, further Y.^ is CO-R 3 , CO-OR3, CONHR 3 , S0 2 R 3 , S0 2 NHR 3 , (CH 2 ) p -0-R 3 , or (CH 2 )_-0-(CH 2 ) m -0-R 3 , where p, and m, are integers and are independently 1 to 20 and R 3 is H, C^-C^ alkyl, c l~ c 2 0 alkenyl containing one or more olefinic bonds, phenyl, halogen substituted phenyl or C- ^ -C^ alkyl substituted phenyl, with the proviso that when Y.^ is CO-OR 3 or CONHR 3 then R 3 is not hydrogen; Y 2 is H, an alkyl group of 1 to 25 carbons, phenyl, naphthyl, phenyl (C-^ - C 2Q ) alkyl-, naphthyl (C*-, - C 20 )alkyl-, halogen substituted phenyl, C-^-Cg alkyl substituted phenyl, halogen substituted naphthyl, C*-,-C 6 substituted naphthyl. The present invention also covers salts of the above-defined compounds, formed with pharmaceutically acceptable acids or bases, as applicable. In a second aspect the present invention relates to pharmaceutical formulations comprising one or more compounds of Formula 1 (or pharmaceutically acceptable salts thereof) in admixture with a pharmaceutically acceptable excipient, for the purpose of treating certain conditions, syndromes or diseases in mammals, including humans. The compounds of the invention have anti-inflammatory, immunosuppressant and anti- proliterative activity without having significant contact sensitizing activity on the mammalian skin. Therefore, the compounds are useful for treating in mammals (including humans) inflammation, rheumatoid arthritis, osteoarthritis, rheumatic carditis, ocular and dermal inflammatory diseases, autoimmune diseases such as allergic diseases, bronchial asthma and myasthenia gravis, and for suppressing unwanted immune responses and retarding proliferation of cell. In still another aspect, the present invention relates to the processes of making the compounds of Formula 1. In general terms, these processes, shown in a summarized fashion in Reaction Scheme 1 comprise the step of reducing a compound of Formula 2 to provide a compound of Formula 1. As the reaction scheme shows, this reduction results in the replacement of the 5- hydroxy function of the 2-furanone moiety with a hydrogen group, thereby providing a "5-deshydroxy" compound. Generally speaking, the compounds of Formula 2 are obtained in accordance with the teachings and synthetic procedures described in United States Patent No. 5,183,906 the specification of which is hereby expressly incorporated by reference. Compounds of Formula 1 where the ^ group is other than hydrogen can also be obtained by introducing the Y-^ substituent into the corresponding hydroxy compound (where Y 1 is hydrogen) by alkylation, acylation reaction with an isocyanate, or other reaction known in the art and appropriate to introduce the Y-_ group into an alcohol such as the Y 2 CH (OH) -moiety of the substituted furanones of this invention. FORMULA2 FORMULA1 Reaction scheme 1 General Embodiments Definitions The terms "ester", "amine", "amide", "ether" and all other terms and terminology used here, (unless specifically defined in the present description) refer to and cover any compounds falling within the respective term as that term is classically used in organic chemistry. Unless specifically noted otherwise, preferred esters are derived from the saturated aliphatic alcohols or acids of ten or fewer carbon atoms or from the cyclic or saturated aliphatic cyclic alcohols and acids of 5 to 10 carbon atoms. Particularly preferred aliphatic esters are those derived from lower alkyl acids or alcohols. Also preferred are the phenyl or lower alkylphenyl esters. The term "alkyl" as used in the present description and claims includes straight chain alkyl groups, branched chain alkyl groups, cycloalkyi groups, alkyl substituted cycloalkyi groups, and cycloalkyi substituted alkyl groups. Unless the number of carbons is otherwise specified, "lower alkyl" means the former broad definition of "alkyl" groups but with the restriction that the group has 1 to 6 carbon atoms. Unless specifically noted otherwise, the term "long chain alkyl" also means the former broad definition of "alkyl" groups but with the restriction that the group has no less than 4 carbon atoms, and no more than approximately 25 carbon atoms. Unless specifically noted otherwise, preferred amides are the mono- and di-substituted amides derived from the saturated aliphatic radicals of ten or fewer carbon atoms, or the cyclic or saturated aliphatic- cyclic radicals of 5 to 10 carbon atoms. Certain compounds of the invention contain a chiral center at the alpha carbon in the side chain on the 4-position of the 2 (5H) -furanone moiety. Other compounds of the invention may contain more than one chiral center. Accordingly, the compounds of the invention may be prepared as mixtures of enantiomeric compounds (where the enantiomers may or may not be present in equal amounts) or as optically pure enantiomers. When there is more than one chiral center, the compounds of the invention may also be prepared as mixtures of diastereomers, or as pure diastereomers, and each diastereomer itself may be a mixture of enantiomers in 1:1, or other, ratios. Alternatively, each diastereomeric compound may be sterically and optically pure. However, all of the above-noted forms, including optically pure enantiomers and mixtures thereof, as well as all diastereomers, are within scope of the present invention. Some of the compounds of the invention may have cis and trans stereoisomers. The scope of the invention includes both pure stereoisomers as well as mixtures thereof. A pharmaceutically acceptable salt may be prepared for any compound of this invention having a functionality capable of forming such salt, for example an acid or an amine functionality. A pharmaceutically acceptable salt may be any salt which retains the activity of the parent compound and does not impart any deleterious or untoward effect on the subject to which it is administered and in the context in which it is administered. Such a salt may be derived from any organic or inorganic acid or base. The salt may be a mono or polyvalent ion. Of particular interest where the acid function is concerned are the inorganic ions, sodium, potassium, calcium, and magnesium. Organic amine salts may be made with amines, particularly ammonium salts such as mono-, di- and trialkyl amines or ethanol amines. Salts may also be formed with caffeine, tromethamine and similar molecules. Where there is a nitrogen sufficiently basic as to be capable of forming acid addition salts, such may be formed with any inorganic or organic acids or alkylating agent such as methyl iodide. Preferred salts are those formed with inorganic acids such as hydrochloric acid, sulfuric acid or phosphoric acid. Any of a number of simple organic acids such as mono-, di- or tri-acid may also be used. The preferred compounds of the present invention, with reference to Formula 1 and with respect to the R^_ substituent are those where R*- ^ is alkyl, more preferably alkyl of 1 to 6 carbons, and stil more more preferably methyl. Also preferred are the compounds where R is phenyl. Regarding the Y^ substituent, compounds are preferred in accordance with the present invention where the Y^ is H, or an acyl group, designated CO-R 3 in Formula 1. More preferably, the acyl group is acetoxy, or a long chain lipophilic acyl group where the R 3 group is long chain alkyl. Especially preferred are compounds where the acyl group is lauroyl. Regarding the Y 2 substitutent, compounds are preferred in accordance with the present invention where Y 2 is long chain alkyl, preferably alkyl having 8 to 16 carbons, even more preferably alkyl having 12 carbons. Compounds are also preferred in accordance with the present invention where Y 2 is hydrogen, provided the Y-^ group is then a long chain lipophilic acyl group. The most preferred compounds of the invention are listed below with reference to Formula 1: Compound 1: n=l, R 1 =5-methyl, Y 1 = CH 3 CO; Y 2 =(CH 2 ) 1;L -CH3 Compound 2: n=l, R 1 =3-methyl, Y 1 = CH3CO; Y 2 =(CH 2 ) n~ H 3 Compound 3: n=l, R 1 =5-methyl, Y 1 = H; Y 2 =(CH 2 ) 11 -CH 3 Compound 4: n=2, R 1 =3-phenyl, R 1 =5-methyl, Y 1 =CO(CH 2 ) 10 CH 3 , Y 2 =H The compounds of the present invention are useful in pharmaceutical compositions to produce anti- inflammatory, immunosuppressant and anti-proliferative activity. The diseases, syndromes or conditions of mammals (including humans) which can be treated with pharmaceutical compositions containing one or more compounds of the invention (or salts thereof) include: inflammation, rheumatoid arthritis, osteoarthritis, rheumatic carditis, ocular and dermal inflammatory diseases, autoimmune diseases such as allergic diseases, bronchial asthma and myasthenia gravis, unwanted immune responses and unwanted proliferation of cells, psoriasis, acne, atopic diseases and allergic conjunctivitis. The compounds of this invention are tested as inhibitors of the enzyme phospholipase A 2 in vitro, and for reduction of inflammation in the mouse ear anti- inflammatory assay in vivo. The compounds of the invention modify calcium homeostasis. This activity is shown by effect on intracellular calcium levels in experiments using gastric glands, spleen cells, epithelial cells, GH 3 cells, etc. Calcium is inhibited from entering through the plasma membrane calcium channels and calcium release from intracellular stores is also blocked. Modification of calcium homeostasis is expected to have application in diseases of the nervous system involving modification of membrane lipids or transmitter release (Parkinson's, Alzheimer's), diseases of the cardiovascular system involving application of cardiac or vascular smooth muscle contractility and platelet aggregation (hypertension, cardiac infarction and atherosclerosis) , diseases of the gastrointestinal tract such as ulcer disease, diarrhea, motility due to secretion of acid or Cl~, diseases of the kidney involving renal handling of fluid and electrolytes (metabolic acidosis, alkalosis) , and disease of abnormal growth (neoplasia, psoriasis) . The compounds of this invention have activity which is similar to that of manoalide, that is the compounds appear to be devoid of the endocrine properties of the glucocorticoids while having anti- inflammatory and immunosuppressive properties. In addition those compounds of the invention which have a substituent in the 5 position lack certain disadvantageous skin-sensitizing properties of certain 5-hydroxy-2 (5H) -furanone derivatives which otherwise have anti-inflammatory activity. Still further, as the data below indicate the compounds of the invention are essentially inactive in the phospholipase A 2 assay, but active in the Ca ++ channel inhibition assay. Thus, the compounds are selective inhibitors of the Ca ++ channel. While, generally speaking activity, in either the phospholipase A 2 or in the Ca ++ channel assay are hallmarks of anti-inflammatory activity, selectivity for Ca ++ channel inhibition is also a highly desirable pharmacological property. The Ca ++ channel selectivity of the compounds of the present invention is unusual and certainly surprising in light of the known prior art. In the methods of this invention, the compounds of the invention are administered to mammals, including humans, in an effective amount to produce the desired activity, preferably in an amount of about 0.05 to 100 mg per day per kilogram of body weight. The amount of the compound depends upon the disease or condition being treated, the severity thereof, the route of administration and the nature of the host. The compounds may be administered topically, orally, parenterally or by other standard routes of administration. Pharmaceutical compositions of this invention comprise compounds of Formula 1, and pharmaceutical carriers suitable for the route of administration. Standard methods for formulating pharmaceutical compositions of this type may be found in Remington's Pharmaceutical Sciences. Mack Publishing Company, Easton, PA. For topical administration, the pharmaceutical composition may be in the form of a salve, cream, ointment, spray, powder or the like. Standard pharmaceutical carriers for such compositions may be used. Preferably, compositions for topical administration will contain 0.05-5% of the active ingredient. A typical cream formulation may contain the following: Ingredient Parts by Weight Water/glycol mixture 50-99 (15% or more glycol) Fatty alcohol 1-20 Non-ionic surfactant 0-10 Mineral oil 0-10 Typical pharmaceutical adjuvants 0-5 Active ingredient 0.05-5 A typical ointment formulation may contain the following: Ingredients Parts by Weight White petrolatum 40-94 Mineral oil 5-20 Glycol solvent 1-15 Surfactant 0-10 Stabilizer 0-10 Active ingredient 0.05-5 For oral administration, suitable pharmaceutical carriers include mannitol, lactose, starch, magnesium stearate, talcum, glucose and magnesium carbonate. Oral compositions may be in the form of tablets, capsules, powders, solutions, suspensions, sustained release formulations, and the like. A typical tablet or capsule may contain the following: Ingredients ~ Percent w/w Lactose, spray-dried 40-99 Magnesium stearate 1-2 Cornstarch 10-20 Active ingredient 0.001-20 Parenteral compositions are prepared in conventional suspension or solution forms, as emulsions or as solid forms for reconstruction. Suitable carriers are water, saline, dextrose, Hank's solution, Ringer's solution, glycerol, and the like. Parenteral administration is usually by injection which may be subcutaneous, intramuscular or intravenous. The compounds of this invention may be combined with other known anti-inflammatory/immunosuppressive agents such as steroids or non-steroidal anti- inflammatory agents (NSAID) in the pharmaceutical compositions and methods described herein. The assay procedures by which useful biological activity of the compounds of the invention can be tested and/or demonstrated, are described below. Calcium Channel (mobilization) Inhibition Assay Polymorphonuclear leukocytes (PMNa) , gastric glands, GH 3 cells, A431 cells, spleen cells, human keratinocytes corneal cells, etc. were loaded with the Ca _?+ sensi*ti*ve fluorescent dye, Fura-2. The appropriate cell type was chosen and the potency and efficacy of the anti-inflammatory furanones on calcium mobilization, calcium channel inhibition was quantitated. The methods used for A431 cells listed below are representative of those used for other cells. A431 cells were detached using a 5-10 min trypsin- EDTA treatment whereas GH 3 cells were treated 2 to 5 min with a 1% pancreatin solution. Cells were immediately washed twice in a 20mM HEPES buffer (pH 7.4) containing 120mM NaCI, 6 mM KC1, 1 mM MgS0 4 , 1 mg/ml glucose and 1 mg/ml pyruvate and 1.4mM calcium (medium A) . Approximately 5 x 10 cells were suspended in medium A and incubated with 4uM fura-2-AM for 15 min at 37°C. After washing the fura-2 loaded cells, the uptake of dye was checked using fluorescence microscopy and found to be evenly distributed in the cytosol of all cells. Fluorescence was continuously recorded with a Perkin-Elmer LS-5 spectrofluorometer. The excitation wavelength was set at 340nm and emission wavelength set at 500nm. The cell suspension was continually stirred, maintained at 37°C and equilibrated for approximately 5 min before addition of various agents. [Ca i was calculated using the following formula: CCa 2-1" ^ = 220 X " Fmin Fmax - F All fluorescence values were measured relative to a EGTA-quenched signal determined as follows: F was the relative fluorescence measurement of the sample. F max was determined by lysing the cells with digitonin (lOOug/ml) in DMSO. After F lnaχ was determined the pH was adjusted to 8, with NaOH and Ca chelated with 3mM EGTA to totally quench the fura-2 signal and obtain When quin-2- was used, cells were incubated with lOuM quin-2- at 37°C for 1 hour, washed and then used. Mouse Ear Anti-Inflammatory Assay 5 Test compound and phorbol myristate acetate (PMA) are topically applied simultaneously to the pinnae of the left ears of mice. PMA alone is applied to the right ear. Three hours and 20 minutes after application, the mice are sacrificed, left and right 10 ears removed, and standard sized bores taken. Edema (inflammation) is measured as the difference in weight between left and right ears [Van Arman, C.G., Clin Pharmacol Ther (1974) 16:900-904]. Inhibition of Phospholipase A 2 -c The effect of compounds of this invention on bee venom phospholipase A 2 is determined by the following procedure: a. Bee venom phospholipase A 2 in 10 uM HEPES (pH 7.4) 20 with 1 mM CaCl 2 is incubated with vehicle or test agent for 1.0 hour at 41°. b. 1.36 mM phosphotidylc oline, 2.76 mM Triton X-100 are dispersed' in buffer by sonication and 25 then mixed with L-3 phosphotidylcholine, 1- palmitoyl-2- (1- 14 C) palmitoyl for 10 min. c. Start the reaction by the addition of enzyme 30 (0.495 units/ml). d. Incubation for 15 sec. at 41°. e. Reaction is terminated by addition of 2.5 ml of isopropanol: n-heptane: 0.5 M H 2 S0 4 (40:10:1; v:v:v: ) , f. 2.0 ml n-heptane and 1.0 ml H 2 0 added; mixture centrifuged. g. 2.0 ml n-heptane removed and treated with 200-300 mg of silica gel HR60. h. Samples centrifuged; 1 ml of n-heptane SN removed and added to 10 ml scintillation fluid, i. Samples counted on a scintillation counter. Activity Data In the above-described phospholipase A 2 assay and Calcium channel mobilization assay the compounds of the invention were found to provide 50% inhibition (IC 50 ) at the following concentrations (in micromoles) , as indicated in Table 1. Table 1 Phospholipase A 2 Assay. Compound name or number IC 50 (micromolar) 1 inactive at 3μM 2 inactive at 3μM 3 inactive at 3μM 4 inactive at 3μM manoalide 0.03 Calcium ,2''+ Channel Mobilization Inhibition Assay Compound name or number IC 50 ( m i crom °l ar ) TRH induced KC1 induced 1 6.4 0.36 2 1.0 0.25 3 0.67 0.47 4 inactive 8.0 manoalide 0.6 0.8 Data for manoalide are provided for comparison. SPECIFIC EMBODIMENTS The compounds of the present invention can be made by the synthetic chemical pathways which are illustrated here in general terms, by reference to United States Patent No. 5,183,906 (the specification of which is expressly incorporated herein) and in the below given specific examples. The synthetic chemist will readily appreciate that the conditions described here in general terms, and specifically, can be generalized to any and all compounds represented by Formula 1. Furthermore, the synthetic chemist will readily appreciate that the herein described synthetic steps may be varied or adjusted by those skilled in the art without departing from the scope and spirit of the invention. Generally speaking, the the compounds of the present invention which, in accordance with Formula 1 lack the 5-hydroxy function on the furan nucleus, can be obtained by reducing with sodium borohydride (or with a comparable mild reducing agent) the corresponding 5-hydroxy-2-furanone compounds. This general reaction is illustrated above in Reaction Scheme 1. The 5-hydroxy 2-furanone compounds of Formula 2 which serve as starting materials for the reduction illustrated in Reaction Scheme 1 can be obtained in accordance with the synthetic procedures described in United States Patent No. 5,183,906 ('906 patent). Specifically, Reaction Scheme 2 and the accompanying pertinent description of the '906 patent disclose a synthetic process for preparing the 5-alkyl or phenyl substituted 5-hydroxy-2-furanone derivatives which can serve as the appropriately substituted starting materials of Formula 2 in Reaction Scheme 1 of the present disclosure. Reaction Scheme 3 and the accompanying pertinent description of the '906 patent disclose a synthetic process for preparing the 3-alkyl substituted 5-hydroxy-2-furanone derivatives which can serve as the appropriate starting materials of Formula 2 in Reaction Scheme 1 of the present disclosure. The starting materials of Formula 2 for the preparation of compounds of the present invention which are 3,5-dialkyl-2-furanone derivatives, can be made in accordance with the description provided in column 13 of the reference '906 patent. The starting materials of Formula 2 for the preparation of compounds of the present invention which are 3-phenyl and 3-phenyl-5-alkyl-2-furanone derivatives, can be made in accordance with Reaction Schemes 4 and 5, respectively, and the attendant description and examples provided in the reference '906 patent. As is described in the reference '906 patent, preparation of the 5-hydroxy-2-furanones starting materials of Formula 2 (of the this disclosure) normally includes a step of oxidation with singlet oxygen, which step utilizes Rose Bengal dye as a catalyst and requires irradiation by light. Generally speaking, the step of singlet oxidation is conducted on a precursor compound of Formula 2 which has a Y^ substituent other than hydrogen, for example an alkyl or an acyl group. The Y^ group is introduced with an alkylating, acylating, alkylsulfonylating reagent, with an isocyanate or with other appropriate reagent, to the hydroxyl function in the side chain of the 4-position of the furan nucleus. As it will become apparent from the ensuing specific examples, the Y j _ can also be introduced by an alkylating, acylating or other appropriate reagent to the hydroxyl function in the side chain of the 4-position on the furan nucleus after singlet oxidation, and after the reduction step pursuant to Reaction Scheme 1 has already been performed. The reduction step of Reaction Scheme 1 can, generally speaking, be conducted on compounds of Formula 2 where Y.^ is either hydrogen or an alkyl or an acyl group. This reaction is typically conducted in an anhydrous ether-type solvent, such as tetrahydrofuran or ethylene glycol dimethyl ether, under a blanket of inert gas, such as argon, at room te parature or at 0° C, typically for 2 or 3 hours. Compound 5 Compound 6 NaBH 3 Compound 5 Compound 7 Reaction Scheme 2 The compound 4-dodecoyloxymethyl-5-hydroxy-5- methyl-3-phenyl-2-furanone (Compound 5) serves as the starting material for the preparation of Compound 4 of the present invention. The preferred synthetic procedure, illustrated in Reaction Scheme 2, for obtaining this compound involves the step of saponifying Compound 5, followed by reduction with sodium borohydride of the resulting 4-hydroxymethyl-5- hydroxy-5-methyl-3-phenyl-2-furanone (Compound 6) to yield Compound 7. Compound 7 is then subsequently re- esterified by treatment with lauroyl chloride to yield Compound 4 of the invention. The starting material 4-dodecoyloxymethyl-5- hydroxy-5-methyl-3-phenyl-2-furanone (Compound 5) is described in the reference '906 patent (see Column 27, lines 7 - 30) and can be prepared in accordance with the synthetic procedure disclosed in the reference '906 patent. The presently prefered procedure for obtaining relatively larger quantitities of Compound 5 differs, however, in certain steps and conditions from the procedure disclosed in the reference '906 patent, and is therefore described here in connection with Reaction Scheme 3. Compound 10 Compound 11 Compound 13 Compound 12 Compound 14 Compound 15 Singlet Oxygen Compound 5 Reaction Scheme 3 In accordance with Reaction Scheme 3, 4-phenyl-3- furylcarbinol (Compound 10) is oxidized to yield 4- phenyl-3-furaldehyde (Compound 11) , which is thereafter reacted sequentially with lithium morpholide, sec-butyl lithium and trimethylsilyl chloride to yield 4-phenyl-5-trimethylsilyl-3-furancarboxaldehyde (Compound 12) . The first compound in this scheme, 4- phenyl-3-furylcarbinol (Compound 10) is described in the reference '906 patent (see column 24) and can be obtained from commercially available starting materials, in accordance with the procedure described in that patent, and also described here. 4-Phenyl-5-trimethylsilyl-3-furancarboxaldehyde (Compound 12) is reduced with lithium aluminum hydride to yield the corresponding alcohol (Compound 13) , which is thereafter methylated with methyl iodide in the presence of n butyl lithium and lithium chloride in the 5-position of the furan nucleus to yield 5- trimethylsilyl-4-phenyl-3-hydroxymethyl-2-methylfuran (Compound 14) . 5-Trimethylεilyl-4-phenyl-3- hydroxymethyl-2-methylfuran (Compound 14) is esterified by treatment with lauroyl chloride in the presence of triethylamine to yield 5-trimethylsilyl-4-phenyl-3- dodecoyloxymethyl-2-methylfuran (Compound 15) . Compound 15 is subjected to singlet oxidation, in a chilled (approximately -5 °0 C) dry tetrahydrofuran solution, in the presence of Rose Bengal dye under irradiation with light to provide 4-dodecoyloxymethyl-5-hydroxy-5-meth- yl-3-phenyl-2-furanone (Compound 5) . The following examples of specific compounds of the invention, and specific examples of the synthetic steps in which the compounds and certain intermediates are made, are set out to illustrate the invention, not to limit its scope. Specific Examples 4- (1-Acetoxytridecyl) -3-methyl-2-furanone (Compound 2) NaBH 4 (25 mg, 0.67 mmol) was added to a solution of 4-(l-acetoxytridecyl) -3-methyl-5-hydroxy-2 (5H) - furanone (157 mg, 0.44 mmol) in anhydrous THF (20 mL) at room temperature under argon. After 2 hours aqueous HCL (1 N) was added carefully until pH2 was reached and the resulting mixture was stirred an additional 1 hour. This mixture was extracted with ethyl acetate and the combined organic fractions were washed with 5% sodium bicarbonate and brine. Evaporation of the dried (magnesium sulfate) extracts gave an oil which was purified by flash chromatography on silica using 30% ethyl acetate/hexane. Fractions with R f of about 0.45 on evaporation afforded the title compound. IR (CHC1 3 ): 1753 cm -1 . 1 H NMR (CDCI3) : 0.88 (t, J = 6.7 Hz, 3H) , 1.19 - 1.38 (m, 20H) , 1.58 - 1.72 (m, 1H) , 1.73 - 1.88 (m, 1H) , 1.91 (m, 3H) , 2.09 (s, 3H) , 4.66 (dd, J = 17.2 Hz, J = 2.0 Hz, 1H) , 4.77 (dd, J = 17.2 Hz, J = 2.0 Hz, 1H) , 5.69 (t, J ■= 6.9 HZ, 1H) . 13 C NMR (CDCI3) : 8.9, 14.0, 20.6, 22.5, 24.9, 29.0, 29.1, 29.2, 29.3, 29.4, 29.5, 31.8, 33.1, 68.9, 69.3, 124.6, 156.7, 169.8, 174.4. Elemental analysis: theoretical carbon 70.97%, found 70.60%, theoretical hydrogen 10.12%, found 9.91%. 4- (1-Hydroxytridecyl) -5-hydroxy-5-nnethyl-2-furanone LiOH (0.5M, 22.6 mL, 11.32 mmol) was added to a solution of 4-(l-acetoxytridecyl) -5-hydroxy-5-methyl-2- furanone (1.00 g, 2.83 mmol) in THF (15 mL) . After stirring for 3 hours at room temperature the reaction was quenched with glacial acetic acid (680 mg, 11.3 mmol) and extracted with ethylacetate. The combined organic fractions were washed with a saturated solution of aqueous sodium bicarbonate, H 2 0 and brine. Evaporation of the dried (magnesium sulfate) extracts gave an oil which was purified by flash chromatography on silica using 40% ethyl acetate/hexane to give the title alcohol. IR (CHC1 3 ): 3376 (br) , 1761 cm -1 1 H NMR (CDCI3) , mixture of diastereomers: 0.88 (t, J = 6.7 HZ, 3H) , 1.2-1.6 (m, 20H) , 1.65-1.90 (m, 5H) , 2.4 (brs, 0.5H), 2.9 (brs, 0.5H), 4.3 (brs, 0.5H), 4.56 (brt, J = 5.0 Hz, 1H) , 4.8 (brs, 0.5H), 5.96 (S, 1H) . 13 C NMR (CDCI 3 ) , mixture of diastereomers: 14.1, 22.8, 24.2, 25.3, 29.3 29.5, 29.6, 31.9, 35.5, 66.7, 66.9, 68.0, 107.2, 107.3, 116.5, 171.2, 173.7. Elemental analysis: theoretical carbon 69.19%, found 69.08%, theoretical hydrogen 10.33% found 10.60%. 4-(l-Hvdroxytridecyl) -5-methyl-2 (5H) -furanone (Compound 3) NaBH 4 (210 mg, 5.54 mmol) was added in portions to a solution of 4-(1-hydroxytridecyl) -5-hydroxy-5-methyl- 2-furanone (586 mg, 1.85 mmol) in anhydrous ethylene glycol dimethyl ether (60 mL) at 0°C under argon. After stirring for 3 hours at 0°C the reaction was quenched with HCI (6 N) to pH2 and extracted with ethyl acetate. The combined organic fractions were washed with a saturated solution of aqueous sodium bicarbonate, H 2 0 and brine. Evaporation of the dried (magnesium sulfate) extracts gave an oil which was purified by flash chromatography on silica using 30% ethyl acetate/hexane to give the title compound as a mixture of diastereomers. Recrystallization from diethyl ether/hexane gave a single diastereomer. t, J = 6.7 Hz, 3H) , 1.2 - 1.5 (m, 20H) , 1.53 (d, J = 6.7 Hz, 3H) , 1.65 (brs, 1H) , 1.66 - 1.86 (m, 2H) , 4.51 (m, 1H) , 5.20 (q, J = 6.7 Hz, 1H) , 5.86 (s, 1H) . ■ " ■ H NMR (CDCI3) : 14.1, 18.7, 22.7, 25.1, 29.3, 29.4, 29.5, 29.6, 31.9, 35.6, 67.7, 79.8, 115.2, 172.8, 175.3. Elemental analysis; theoretical carbon 71.83%, found 72.09%, theoretical hydrogen 10.89%, found 10.91%. 4-d-Acetoxytridecyl) -5-methyl-2 (5H) -furanone (Compound 1) 4- (1-Hydroxytridecyl) -5-methyl-2 (5H) -furanone (Compound 3, 329 mg, 1.11 mmol) , acetic anhydride (453 mg, 4.45 mmol) , triethylamine (227 mg, 2.22 mmol) and 4-dimethylaminopyridine (2 mg, 0.02 mmol) were stirred at room temperature under argon for 3 hours in anhydrous THF (30 mL) . The crude reaction mixture was concentrated and the residue was taken up in diethyl ether and washed with a 10% aqueous HCI solution, a saturated solution of sodium bicarbonate, H 2 0 and brine. Evaporation of the dried (magnesium sulfate) extracts gave an oil which was purified by flash chromatography on silica using 20% ethyl acetate/hexane to give the title compound. e of diastereomers: 0.88 (t, J = 6.6 Hz, 3H) , 1.2-1.4 (m, 20H) , 1.46 (d, J = 6.8 Hz , 1.5H), 1.52 (d, J = 6.8 Hz, 1.5H), 1.71 (m, 1H) , 1.81 (m, 1H) , 2.10 (s, 1.5H) , 2.14 (s, 1.5H) , 4.97 (dq, J = 6.8 Hz, J = 1.6 Hz, 0.5H) , 5.11 (dq, J = 6.8 Hz, J = 1.6 Hz, 0.5H) , 5.52 (brt, J = 6.6 Hz, 0.5H) , 5.57 (m, 0.5H), 5.89 (m, 0.5H), 5.47 ( , 0.5H). 13 C NMR (CDC1 3 ) , mixture of diastereomers: 13.9, 18.3, 18.5, 20.6, 22.5, 24.6, 24.9, 29.0, 29.1, 29.2, 29.3, 29.4, 31.7, 32.9, 33.4, 68.4, 69.4, 78.3, 79.0, 115.3, 117.3, 169.6, 169.9, 170.6, 171.5, 171.6, 171.7. Elemental analysis: theoretical carbon 70.97%, found 71.09%. Theoretical hydrogen 10.12%, found 10.32%. 4-Hydroxymethyl-5-hydroxy-5-methyl-3-phenyl-2-furanone (Compound 6) LiOH (0.5 M, 19.7 L, 9.83 mmol) was added to a solution of 4-dodecoyloxymethyl-5-hydroxy-5-methyl-3- phenyl-2-furanone (Compound 5, see improved process for preparation below) , in THF (20 mL) at room temperature. After stirring for 3 hours the reaction was quenched with glacial acetic acid (590 mg, 9.83 mmol) and the THF was removed by rotary evaporator. Solids were removed from the residual aqueous slurry. The clear aqueous solution containing the crude product was lyophylized, adhered to silica with methanol and purified by flash chromatography using 60% ethyl acetate/hexane to give the title alcohol. IR (CHCI3): 3300, 3023, 1766 cm -1 . 3-H NMR (CDCI3) : 1.76 (s, 3H) , 3.3 (vbrs, 1H) , 4.57 (s, 2H) , 5.7 (vbrs, 1H) , 7.32 to 7.42 ( , 5H) . 13 C NMR (CDCI3) : 24.2, 56.2, 105.5, 128.3, 128.5, 129.1, 129.3, 158.3, 170.5. LRMS: m/x observed at 220 (M + ) . 4-Hydroxymethyl-5-methyl-3-phenyl-2 (5H) -furanone (Compound 7) To NaBH 4 (147 mg, 3.88 mmol) suspended in anhydrous ethylene glycol dimethyl ether (20 mL) at 0°C under argon was added 4-hydroxymethyl-5-hydroxy-5- methyl-3-phenyl-2-furanone (Compound 6) in ethylene glycol dimethyl ether (20 L) dropwise. After stirring for 3 hours the solution was acidified to pH2 with 6N HCI, warmed to ambient temperature and stirred an additional 1 hour. The solution was concentrated to 10 L volume and the organics were extracted into ethyl acetate. The combined organic fractions were washed with a saturated solution of aqueous sodium bicarbonate, H 2 0 and brine. Evaporation of the dried (magnesium sulfate) extracts gave an oil which was purified by flash chromatography on silica using 30% ethyl acetate/hexane to give the title compound. IR (CHC1 3 ) : 3616, 3300 (v. br.) , 3022, 1747 cm "1 . 1 H NMR (CDCI3) : 1.54 (d, J = 6.8 Hz, 3H) , 3.38 (m, 1H) , 4.54 (dd, J = 6.1 Hz, J = 14.9 Hz, 1H) , 4.70 (dd, J = 3.9 Hz, J = 14.9 Hz, 1 H) , 5.24 (q, J = 6.8 Hz, 1H) , 7.30 - 7.43 (m, 5H) . 13 C NMR (CDCI3) : 18.3, 57.0, 78.2, 126.0, 128.4, 128.8 129.1, 164.6, 172.9. LRMS: m/z observed at 204 (M + ) . 4-Dodecoyloxymethyl-5-methyl-3-phenyl-2 C5H) -furanone (Compound 4) 4-Hydroxymethyl-5-methyl-3-phenyl-2 (5H) -furanone (Compound 7) , (73 mg, 0.36 mmol), lauroyl chloride (117 mg, 0.54 mmol) , triethylamine (47 mg, 0.47 mmol) and dimethylaminopyridine (1 mg, 0.01 mmol) were stirred in anhydrous THF (2 L) at ambient temperature under argon for 2 hours. The crude reaction mixture was concentrated, the organics were extracted into diethyl ether and solids were filtered off. The ether was removed by rotary evaporator and the resulting oil was purified by flash chromatography on silica to give the title compound. IR (CHCI3): 3027, 1751 cm "1 . ^ ■ H NMR (CDCI3): 0.88 (t, J = 6.6 Hz, 3 H) , 1.15 to 1.37 (m, 16 H) , 1.56 (d, J = 6.7 Hz, 3 H) , 1.61 (m, 2H) , 2.32 (m, 2H) , 5.07 (d, J = 4.4 Hz, 2H) , 5.15 (q, J = 6.7 Hz, 1H) , 7.36 - 7.49 (m, 5 H) . 13 C NMR (CDCI3): 14.0, 18.4, 22.6, 24.7, 29.0, 29.1, 29.2, 29.3, 29.5, 31.8, 33.8, 57.9, 77.4, 128.5, 128.8, 128.9, 129.1, 129.2, 157.6, 171.3, 172.9. Elemental analysis: theoretical carbon 74.58%, found 74.58%; theoretical hydrogen 8.87%, found 8.59%. Improved Preparation of 4-dodecoyloxymethyl-5-hydroxy-5-methyl-3-phenyl-2-furanone (Compound 5) Step 1. Preparation of 4-Phenyloxazole. Amount MW moles 2-Bromoacetophenone 40 g 199.05 0.2 Ammonium Formate 44 g 63.06 0.7 Formic Acid 210 mL A mixture of 2-bromoacetophenone and ammonium formate in formic acid was stirred at reflux under nitrogen for 2 hours. The cooled reaction mixture was treated with 400 mL of H 2 0 and extracted with ether (3 x 200 mL) . The combined organics were washed with saturated aqueous NaHC0 3 , dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (eluting with EtOAc/hexanes, 1:9) to afford 8.9 g (31%) of 4- phenyloxazole as a yellow oil. Step 2. Ethyl-4-phenylfuran-3-carboxylate. Amount MW moles 4-Phenyloxazole 8.86 g 145 0.061 Ethyl phenylpropiolate 10.6 g 174.2 0.061 A mixture of 4 -phenyloxazole and ethyl phenylpropiolate was heated at 200-210"C in a sealed tube under N 2 for 22 hours. The cooled reaction mixture was purified by column chromatography (eluent, CH 2 Cl 2 /hexanes, 1:1) to give 12.65 g (96%) of adduct as a red oil which was contaminated with phenylacetonitrile. Although the product obtained in this preparation was not 100 % pure, it was used in the next step without further purification. Step 3. (4-Phenyl-3-furyl) carbinol (Compound 10) Amount MW moles ethyl-4-phenylfuran-3-carboxylate 12.65 g 216 0.059 LiAlH 4 1.23 g 37.95 0.033 THF 180+30 mL To the solution of ethyl-4-phenylfuran-3-carboxylate in dry THF (180 mL, freshly distilled from Na/benzophenone) was added dropwise the suspension of lithium alumium hydride in THF (30 L) at 0°C under nitrogen. Then the mixture was warmed to room temperature and quenched with IN HCI acid and extracted with EtOAc. The organic layer was washed with brine, dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (eluent, CH 2 C1 2 ) to afford 6.1 g (59%) of (4-phenyl-3-furyl)carbinol as white crystals. Step 4. 4-Phenyl-3-furancarboxaldehyde (Compound 11) Amount MW moles Compound 10 9.39 g 174 0.054 DMSO 8.44 g (7.7 mL) 78.13 0.108 TFAA 20.94 g (28 mL) 210.03 0.081 iPr 2 NEt 20.94 g (28 mL) 129.25 0.162 CH 2 C1 2 60+10+40 mL To a solution of DMSO (distilled from CaH 2 ) in dry CH 2 C1 2 (60 mL, distilled from CaH 2 ) was added dropwise trifluoroacetic anhydride in dry CH 2 C1 2 (10 mL) under nitrogen at -78°C over approximately 15 minutes. The mixture was stirred at -78°C for an additional 15 minutes. To the slurry was then added dropwise the starting material in dry CH 2 C1 2 (40 mL) at -78°C over approximately 20 minutes. The yellow solution was stirred at -78°C for 1 hour. The iPr 2 NEt was then added at -78°C and the solution was warmed gradually to room temperature. The reaction mixture was washed sequentially with IN HCI and saturated aqueous NaHC0 3 , dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (EtOAc/hexanes, 1:4) to give 8.88 g (96%) of 4-phenyl-3-furancarboxaldehyde (Compound 11) as a yellow oil. Step 5. 4-Phenyl-5-trimethylsily1-3-furancarboxaldehyde (Compound 12) To the solution of morpholine (freshly distilled from CaH 2 ) in dry THF (60 mL) was added dropwise n-BuLi at o°C under nitrogen. The resulting solution was stirred at 0°C for an additional 15 minutes then cooled to -78°C, and aldehyde (Compound 11) in dry THF (40 mL) was added dropwise. The mixture was warmed to 0°C and stirred at 0°C for 15 minutes, then the mixture was recooled to -78°C, and the s-BuLi was added dropwise. The resulting slurry was stirred at -78°C and the reaction mixture was gradually warmed to room temperature. The reaction mixture was quenched with saturated aqueous NH 4 C1 and extracted with EtOAc. The organic layer was dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (eluent, EtOAc/hexanes, 1:4) to give 8.08 g (68%) of 4-phenyl-5- trimethylsilyl-3-furancarboxaldehyde (Compound 12) as a yellow oil. Step 6. (4-Phenyl-5-trimethylsilyl-3-furyl) carbinol (Compound 13) To the solution of tri ethylsilyl-aldehyde (Compound 12) in dry THF was added dropwise the LiAlH 4 solution at 0°C under nitrogen. The reaction mixture was stirred at 0°C for 1 hour, then quenched with IN HCI (60 mL) and extracted with EtOAc. The organic layer was washed with brine, dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (EtOAc/hexane, 1:4) to give 6.75 g (83%) of the title compound as a yellow oil. Step 7. 5-Trimethylsily1-4-pheny1-3-hydroxymethy1-2- methylfuran (Compound 14) Amount MW moles Compound 13 6.8 g 246 0.028 n-BuLi, 2.5M in hexanes 25 mL 0.063 LiCl 11.72 g 42.4 0.28 Mel 19.6 g 141.9 0.14 THF 120 mL To the solution of Compound 13 in dry THF was added dropwise n-BuLi at -78 " C under nitrogen. The reaction mixture was gradually warmed to -20°C, and the LiCl was added at -20°C, followed by Mel (freshly distilled) . The mixture was stirred at -20°C for 24 hours, then quenched with saturated aqueous NH 4 C1, and extracted with EtOAc. The organic layer was dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (eluent, EtOAc/hexanes, 1:4) to give 6.37 g of product as a yellow oil. The product was contaminated with the 2- desmethyl compound (starting material) but was suitable for the next reaction step. (Ratio of product to starting material, approximately 4 :1 by proton NMR integration, corresponding to 71% yield of product) . Step 8. 5-trimethylsily1-4-phenyl-3-dodecoyloxymethyl-2-methyl- furan (Compound 15) Amount MW moles Compound 14 6.1 g 260 0.0235 lauroyl chloride 10.26 g 218.77 0.047 Triethylamine 4.75 g 101.19 0.047 THF 100 mL To the solution of Compound 14 in dry THF was added triethylamine at 0°C under nitrogen, followed by lauroyl chloride. The mixture was stirred at 0°C for 1 hour, then treated with water and extracted with ether. The organic layer was washed with brine, dried over MgS0 4 , filtered and concentrated under reduced pressure. The residue was purified by column chromatography (eluent, EtOAc/hexanes, 1:25) to give 12.14 g of product as a colorless oil in quantitative yield. This product was contaminated with its corresponding desmethyl analog. Step 9. - - -h dro -5- eth l-3- hen l-2- The solution of Compound 15 and Rose Bengal in THF was saturated with 0 2 , then cooled to approximately -5°C (dry ice/acetone bath) and irradiated with a 150 watt flood lamp (maintaining the temperature of the mixture at -5 to 0°C) for 3 hours. The reaction mixture was then filtered and concentrated to a pink oil which was purified by column chromatography (eluent, EtOAc/hexanes, .1:20 to 1:4) to give 3.4 g (27%) of pure title compound as a colorless oil. An additional 3 g (approximately 25%) of a mixture of the title compound and the 5-desmethyl derivative (ratio 1:1 by proton NMR integration) was also obtained as a yellow oil.

Claims: AMENDED CLAIMS [received by the International Bureau on 31 August 1995 (31.08.95); original claim 1 amended; remaining claims unchanged (1 page)] 1. (AMENDED) A compound of the formula STATEMENT UNDER ARTICLE 19 Applicant has amended the parent claim 1 in order to set forth that within the scope of this claim and all claims dependent from it, the 5 position of the 2- furanone nucleus is always substituted with the Ri group, which has been amended to exclude H. The difference between the subject matter of the Lee reference (United States Patent No. 5,045,564) and the amended claims is that the l___n_ compounds are unsubstituted in the 5 position of the furanone nucleus. This accounts for significant difference in the response of the two groups of compounds to skin testing. Applicant has the conducted art accepted skin sensitizing tests according to the published procedures (Maσnusson and liσman. Allergic Contact Dermatitis in the Guinea Pig. Identification of Contact Allergens, Charles C. Thomas, 1970) . The amount of skin sensitization is measured visually and graded in a manner well known in the art. These tests demonstrated that Applicant's compounds which are 5- substituted are a non-sensitizers and that the Lee compounds which are unsubstituted in the 5- position, are strong sensitizers. A declaration by Michael Garεt Ph.D. describing the results of these tests is enclosed. Inasmuch as the lack of the undesirable skin irritating/sensitizing property of the 5-substituted furanone compounds of the invention is unexpected and surprising over the 5-unsubstituted reference compounds, the compounds claimed herein are innovative over the Lee reference. United States Patent No. 5,043,457 and WO 92/19610 differ from the subject matter of the present claims in that in these reference compounds the 5 position of the furanone is either unsubstituted or is substituted with a substituent of different nature than in the present claims.

Inventors: GARST MICHAEL E, GARST, MICHAEL, E.
Applicants: ALLERGAN INC, ALLERGAN, INC.
Application year: 1995
Main classification: C07D307
IPC codes: C07D307