DERIVATIVES OF BENZOPYRAN AND OF BENZOTHIOPYRAN, THEIR PREPARATION AND THEIR USE AS AGONISTS OF DOPAMINERGIC RECEPTORS

Abstract: Compounds of formula (I), wherein R, R', R", Z, m, Y, n, R1, X, p and Ar have the meanings reported in the description; and pharmaceutically acceptable salts thereof are described. The compounds of formula (I) are useful in the treament of arterial hypertension and heart failure, of renal failure, of peripheral arteriopathies, of cerebrovascular insufficiencies and of ischemic cardiopathy.

Description: DERIVATIVES OF BENZOPYRAN AND OF BENZOTHIOPYRAN, THEIR PREPARATION AND THEIR USE AS AGONISTS OF DOPAMINERGIC RECEPTORS 5 The present invention relates to compounds active on the cardiovas¬ cular system and, in particular, it relates to derivatives of benzo- pyran and of benzothiopyran and to their use in therapeutic field. It is known that among the dopamme analogs several cyclization compounds such as, for example, the derivatives of 2-amιno-1 ,2,3,4- 1Q -tetrahydro-naphthalene and their isosters 3-ammo-3,4-dιhydro-2H-l - benzopyrans and benzothioDyran«; ptsin a siΞ ι icart do mine gi activity. On the dopa ine analogs, several studies about the structure-activ¬ ity relationship have been carried out to determine the structural ]5 characteristics able to ensure the best dopaminergic activity and to avoid, at the same time, the undesired effects of dopamine. An interesting review of these studies is collected in a paper published by H.E. Katennopoulos and D.I. Schuster on Drugs of the Future, vol. 12(3), pages 223-253, (1987). Q In spite of the various studies, however, the topology of dopaminergic receptors has not yet been explained and a series of receptor models has been proposed in the last ten years. In the field of the compounds structurally related to dopamine, some authors have found that the presence of a -_.-__. alkyl group on the ammo function is one of the requirements for dopaminergic activity 5 while the structural requirements of the second substituent on the ammo group have not yet been found. Nevertheless, there are several examples in literature showing how the structural features of the two substituents can be, in practice, extremely variable and how small changes of the molecule can affect 0 the pharmacological activity both quantitatively and qualitatively in a relevant manner. Among the most significant examples the following are cited. 5 European patent application no. 72061 (Fisons PLC) describes, among the others, dopa ines and aminotetrahydronaphthalenes having a mono- or di-substituted portion of formula -N-CH_-X-CH_.-N-D_ Q wherein X is a -(CHa) n - chain, optionally substituted by h v droxies; r is ar: integer between 1 and 7; R-, and R_, the same or different, are hydrogen, alkyl or phenyl; D_ is hydrogen, alkyl, phenyl; alkyl substituted by one or more hydroxies, pyridyls, phenyls; alkyl 5 substituted by phenyl substituted, in turn, by halogen, alkyl, amino, alkoxy or nitro; or D_ may be an optionally mono- or di-hy- droxy substituted phenylethyl or tetrahydronaphthyl moiety. Among the compounds described in European patent application No. 72061 , the compound of formula CH 2 -CH_-NH-(CH_) ft -NH-C whose International non-proprietary name is dopexamine (The Merck 5 Index - XI ed., No. 3418, page 538) is the only compound, as far as we know, which has been developed and used in the acute treatment of failure. It is significant that dopexamine, notwithstanding it was selected among the several compounds described and exemplified in European 0 patent application no. 72061 , is an agonist of dopaminergic recep¬ tors less active than dopamine and, like dopamine itself, it is not absorbed when administered by oral route [A. Fitton and P. Benfield, Drugs, 39.(2), 308-330, (1990)]. European patent application no. 142283 (Fisons PLC) describes a class of compounds which are analogs of dopexamine and in which the amino group of the dopamine moiety is still secondary. In literature, there are several compounds with a catecholamine structure having the aim of keeping the favorable properties of ( op p xsmin . ΛISO whe administered by oral route, or of increasing the selectivity towards the dopaminergic receptors. Among these, those described in European patent application No. 321968 (SIMES Societa Italiana Medicinali e Sintetici S.p.A.) having the following general formula: wherein R and R-, , the same or different, are hydrogen or acyl deriving from an aliphatic, aromatic or heteroaromatic carboxylic acid, from a carbonic or carbamic acid or from a phosphoric acid; n_ and __ are integer numbers selected among 0 and 1 ; m_ is an integer number selected among 1, 2, 3 and 4 so that n+p=l and m+n is 2, 3 or 4; R_ and R 3 , the same or different, are hydrogen, halogen, alkyl or alkoxy; are particularly interesting. These compounds are agonists of D-, and D_ dopaminergic receptors, show contemporaneously an α-, -antagonist effect, do not interact with other receptor systems, but in order to be active by oral adminis¬ tration they have to be transformed into suitable pro-αrugs. The compounds described in the International patent application UO 93/19036 (Za bon Group S.p.A.), which are dopaminergic agonists more potent than dopamine and not selective towards any specific receptor sub-type, which do not interact with other receptor systems and whicn, at the same time, do not show either the side effect cr the therapeutically disadvantageous aspects of dopamine, are still more ιnt TP ing The compounds described in the above cited International patent application have the following general formula: wherein Ri and R_, the same or different, are hydrogen atoms or OY' groups; Y and Y', the same or different, are hydrogen atoms or acyl groups deriving from an aliphatic, aromatic or heteroaromatic carboxylic acid, from a carbonic or carbamic acid or from a phosphoric acid; m. is 1 or 2; n is an integer number among 3 and 7; R 3 is hydrogen or Cι-C_ alkyl; R * and R β , the same or different, are hydrogen, halo¬ gen, Cι-C 3 alkyl or alkoxy. However, as far as we know, in the specific field of benzopyran ana benzothiopyran derivatives, compounds having an interesting dopaminergic activity useful in the cardiovascular field, particularly for the treatment of arterial hypertension and heart failure, of renal failure, in the treatment of peripheral arteriopathies, of cerebrovascular insufficiencies and of ischemic cardiopathy have been never described up to now. Ue have now found derivatives of benzopyran and of benzothiopyran which are agonists of dopaminergic receptors more potent than dopa¬ mine and which are particularly useful in cardiovascular field. Therefore, object of the present invention are compounds of formula CH a -CH_-CH 3 (CH a >„,-Y-(CH a )-,-CHRι -X-(CH a ),_-Ar (I) wherein m_ is an integer number selected among 4, 5, 6, 7 and 8; Z is 0, S, SO or S0 a ; R, R' and R" are hydrogen atoms or OH groups, provided that at least one among R, R' and R" is a hydrogen atom but R, R* and R" are not all contemporaneously hydrogen atoms and R' and R" are not both contemporaneously OH groups; or one of R' and R" is a NHCHO, NHCH 3 , NHS0 a CH 3 , CH a 0H or CH 3 group and the other is hydrogen; n. is an integer number selected among 0, 1, 2, 3 and 4; 2. is an integer number selected between 0 and 1 ; Ri is a hydrogen atom or a Ci -C * alkyl group; Ar is a phenyl of formula R " wherein R a , R 3 and R_,, the same or different, are hydrogen, OH, halogen, Ci-C-. alkyl, Ci-C * alkoxy, nitro, Cι-C_ alkylthio, NH a , mono- or di-C-i-C * alkylamino, SH, C, -C_ alkylsulphonyl, Cι-C_ alkoxycarbonyl, NHCHO, C-.-C * alkylcarbonyla ino, NHC0NH 2 , Ci-C * alkylsulphonylammo, Ci-C * alkylammosulphonyl, S0_NH 2 , NHS0 2 NH 2 , C00H, S0 3 H, CONH 2 , CH 2 0H or phenyl; or R a and R 3 , in ortho position one with respect to the other, together form an optionally unsaturated chain made by 3 or 4 groups selected among CR'"R IV , CO, S, 0 and NR V , wherein R"' is a hydrogen atom or a Ci-C * alkyl group, R I is a hydrogen atom, a C n -C_ alkyl group or an am o group and R v is a hydrogen atom or a Ci-C. alkyl group; or R" τ together with a vicinal R"' or R v constitutes a single bond or R v together with a vicinal R τ " or R v constitutes a single bond; or a 5 or 6 membered aromatic heterocycle containing one or more heteroatoms selected among oxygen, nitrogen and sulphur, option¬ ally substituted by from one to three substituents selected among halogen, OH, Ci-C * alkyl or alkoxy; Y is S, 0, N(R β )C0, C0(R B )N or N(R β ); X is N(R»), 0, S, SO, S0 2 , CO or a single bond; Re is a hydrogen atom or a Ci-C * alkyl group; R* is a hydrogen atom; or R β and R A together form a -CH 2 - or -CH_-CH 2 - chain; or R 2 , when in ortho position with respect to X, constitutes a single bond or form a -CH 2 - or -CH 2 -CH 2 - chain with R a ; or when X=0 R a , when in ortho position with respect to X, forms a -CH 2 -0- chain with R, ; provided that when p=i , X is an N(R ft ) group; the asterisk marks an asymmetric carbon atom; and pharmaceutically acceptable salts thereof. Th p compounds of formula I have at least an asymmetric center, marked by an asterisk, and they may be in the form of stereoiso ers. Object of the present invention are the compounds of formula I in the form of stereoiso eric mixtures as well as in the form of single stereoisomers. The compounds of formula I are agonists of dopaminergic receptors active also by oral route and with long duration of action and they are useful in therapy in cardiovascular field, in particular for the treatment of arterial hypertension and heart failure, of renal failure, in the treatment of peripheral arteriopathies, of cerebro¬ vascular insufficiencies and of ischemic cardiopathy. With the term halogen atom we intend a fluorine, chlorine, bromine or iodine atom. Specific examples of alkyl or alkoxy groups are methyl, ethyl, n.propyl, l.propyl, n.butyl, l. utyl, sec.butyl, t.butyl, methoxy, ethoxy, n.propoxy, l.propoxy, n.butoxy and l.butoxy. Specific examples of 5 or 6 membered heteroaryls containing one or more heteroatoms selected among oxygen, nitrogen and sulphur are pyridyl, pyrimidyl, imidazolyl, thiazolyl, furyl, thienyl, oxazolyl, isoxazolyl, piperazinyl and pyrrolyl. Specific examples of optionally unsaturated chains made by 3 or 4 groups selected among CR"'R IV , CO, S, 0 and NR V are the following: -0-CHR"'-0-, -S-C0-NR v -, -CHR'"-C0-NR v -, -S-CR IV =N-, -0-C0-NR v -, -C0-NR v -NR v -, -NR v -C0-NR v -. Preferred compounds of formula I are the compounds wherein R' is a hydrogen atom, R and R" are OH groups. Still more preferred compounds are the compounds wherein R' is a hydrogen atom, R and R" are OH groups and _ is 6. Among the meanings of the substituents R 2 , R 3 and R * , the preferred aro hydrogen, OH, ethoxy, m ethyl, nitre, chlcrc, methyisulphcnyl, NH a , S0 2 NH 2 , methylsulphonylamino, NH 2 C0NH 2 , ethoxycarbonyl, ace- tylamino, C0NH 2 , CH 2 0H and S0 3 H or, when R 2 and R 3 in ortho position one with respect to the other form a chain, a methylendioxy group or a group of formula -S-C0-NR v - wherein R v is a hydrogen atom. Still more preferred among the meanings of R 2 , R 3 and R * are OH, methoxy and -S-C0-NR v - wherein R v is a hydrogen atom. Pharmaceutically acceptable salts of the compounds of formula I are the salts with organic and inorganic acids such as, for example, hydrochloric, hydrobromic, hydroiodic, nitric, sulphuric, phos¬ phoric, acetic, benzoic, maleic, fu aric, succinic, tartaric, cit¬ ric, aspartic, methansulphonic and 3,7-dι-tert.butylnaphthalen-l ,5- disulpnonic acid (dibudinic acid). The preparation of the compounds of formula I can be carried out according to the synthetic method described herein after. The method comprises the reaction between a compound of formula Cπ 2 -CH 2 ~Cr- 3 wherein Z has the already reported meanings; R-7, Re and R 9 are hydrogen atoms or OA groups wherein A is a hydro¬ gen atom or a protective group selected, for example, among methyl, benzyl, benzoyl and 4-methoxybenzoyl, provided that at least one among R-?, R β and R v is a hydrogen atom but R-7, e and R 9 are not all contemporaneously hydrogen atoms and R a and R 9 are not both contemporaneously OA groups; or one of R β and R, is an NHCHO, NHCH 3 , NHS0 2 CH 3 , CH 2 0H or CH 3 group and the other is hydrogen; and an acid of formula H0-C-(CH a > m - 2 -W-Y- -X-<CH_)p-Ar (III) II 0 wherein _ __, X. Y and Ar have the already reported meanings; W is a CH a or CO group; is a group of formula CO-(CH 2 )n-ι-CHRι wherein Ri has the already reported meanings, when n_ is 1 , 2, 3 or 4; or 8 is a CHRi or CO group, wherein Ri has the already reported meanings, when n is 0; or a reactive derivative thereof such as an acyl halide or a mixed anhydride which can optionally be prepared in situ, in an inert solvent and in the presence of a base such as an alkaline carbonate or bicarbonate or a tertiary amine, in order to obtain the intermediate compounds of formula CH_>~CH 2 — CH 3 wherein __, __, Ar, Z, W, Y, Q, X, R- , R β and R„ have the alreaαy reported meanings; and their reduction, preceded or followed by the optional αeprotec- tion of the hydroxy groups, in order to obtain the compounds of formula I. The reduction of the compounds of formula IV can be carried out with electrophile reducing agents, in particular with diborane optionally complexed with dimethylsulphide, tetrahydrofuran, aliphatic amines such as triethylamine or aromatic amines such as N,N-dιethylanilιne or pyridine. Alternatively, the reduction can be carried out with nucleophile reducing agents such as metal hydrides, for example lithium aluminum hydride. The reduction reaction is carried out in a suitable solvent such as for example tetrahydrofuran, diethylether or 1 ,2-dιmethoxyethane. The optional deprotection of the hydroxy groups is carried out according to conventional techniques such as hydrolysis or hydro- genolysis. The compounds of formula II are known or can be prepared according to known methods [Al Neirabeyeh M. et al., Eur. J. Med. Chem. (1991) 26, 497-504]. Also the compounds of formula III are known or easily prepared according to conventional methods. Alternatively, the compounds of formula I wherein Y is a N(R β ) group can be prepared following a different sequence. At first it is carried out the condensation reaction between a compound of formula II and a suitable reactive derivative of an acid of formula H0-C-(CH a )-,-ι-Y-H (V) II o wherein Y is an N(R S ) group; m_ and R β have the already reported meanings; followed by the optional reduction according to what already re¬ ported, in order to obtain the intermediate of formula CH 2 -CH a —CH 3 R- wherein Y is an N(R-) group; m_ Z, U, R β , R--, R β and Rv have the already reported meanings. The intermediate of formula VI is then reacted with a suitable reactive derivative of an acid of formula H0-C-[(CH 2 )r,-ι-CHRι ]_.-X-(CH 2 )p-Ar (VII) Ii o wherein n_ 2_ Ri , Ar and X have the already reported meanings; r_ is 0 or 1 ; obtaining the corresponding intermediates of formula IV. The subsequent reduction, preceded or followed by the optional deprotection of the hydroxy groups, gives the compounds of formula I wherein Y is a N(R B ) group, object of the present invention. A further alternative for the preparation of the compounds of for¬ mula I wherein Y is an N(R β ) group consists in first reacting a compound of formula II with a suitable reactive derivative of a bicarboxylic acid of formula H0-C-(CH 2 ) m _ 2 -C-0H (VIII) II il o o wherein m_ has the already reported meanings; in order to obtain an intermediate of formula wherein __, Z, R-7, Rβ and R, have the already reported meanings. The subsequent condensation with an amine of formula H-Y- ( CH 2 ) r ,-CHRι-X- ( CH a ) I _-Ar ( X ) wherein Y is an N(R S ) group; n_ __, X, Ri and Ar have the already reported meanings; gives the corresponding intermediates of formula IV. The subsequent reduction, preceded or followed by the optional deprotection of the hydroxy groups, allows to obtain the compounds of formula I, object of the present invention. Some of the compounds of formula I, optionally protected, may also be used as intermediates for the synthesis of other compounds of formu]a I by transformation of certain functional groups present in the molecule. Thus, for example, compounds of formula I wherein one or more of R a , R 3 and R * is a nitro group or an alkoxycarbonyl group can be trans- formed into the corresponding compounds of formula I wherein one or more R a , R 3 and R_ is an amino or carboxy group by reduction or hydrolysis respectively. In turn, compounds of formula I wherein one or more of R 2 , R 3 and R * is an amino group or a carboxy group can be 5 transformed into the corresponding compound of formula I wherein one or more of R 2 , r 3 andR * is an alkylcarbonylamino or CH 2 0H gorup by acylation or reduction respectively. Analogously, the same transformation can be carried out on interme¬ diates of formula IV. 0 The compounds of formula I in optically active form are obtained by optical separation or by stereospecific or stereoselective syn¬ thesis. The preparation of salts of the compounds of formula I is carried out according to conventional methods. 5 The compounds of formula I are agonists of Di and D 2 dopaminergic receptors having more affinity than dopamine and than dopexamine as results from the in vitro binding tests. Furthermore, their activity is at least comparable to that of the compounds described in the alreaxy cited International patent appli- 0 cation WO 93/19036. The in vitro results have been confirmed also by functional studies on isolated tissues, which are predictive of the in vivo activity, such as the Rabbit Splenic Artery (RSA) test and the Rabbit Ear Artery (REA) test (example 15). ς The tests for interaction with the other receptor systems showed that the compounds of formula I do not significantly interact and then are endowed with high specificity. The compounds of formula I resulted also to be inactive on the central nervous system after oral administration and this lack of effect is a further positive property generally not shared with the 0 other compounds with cathecolam e structure. It is clear how these characteristics of selectivity and receptor specificity together with the lack of activity on the central nerv- ous system make the compounds of formula I particularly suitable for the treatment of carαiovascular diseases and mainly in the antihy- pertensive therapy, m the therapy of hearth failure, of renal insufficiency, in the treatment of peripheral arteriopathies, of cerebrovascular insufficiencies and of ischemic cardiopathy. In addition to the already underlined higher pharmacologic activity, the feature which more characteri7pς the ro pπunds nf formula I, object of the invention, is their absorbability by oral route ana their prolonged duration of action. As a consequence for the practical uses in therapy, the compounds of formula I can be administered by parenteral route as well as by enteral route so differing from dopamine and from dopexamine. The therapeutic doses will be generally comprised between 5 mg and 1 g a day and between 1 and 300 mg by oral route for each single administration. Tne pnarmaceutical compositions containing a therapeutically effec¬ tive amount of the compounds of formula I or of their pharmaceuti¬ cally acceptable salts in admixture with a suitable carrier are, furthermore, object of the present invention. The pharmaceutical compositions object of the invention may be liquid, suitable for enteral or parenteral administration, and, preferably, solid such as tablets, capsules, granulates, suitable for oral administration. The preparation of the pharmaceutical compositions object of the invention can be carried out according to traditional techniques. Notwithstanding the compounds of formula I are active as such also when orally administered, in order to fulfill some specific thera¬ peutic or pharmaceutical requirements it can be useful to transform them into the corresponding pro-drugs. According to the techniques used in the field of phenolic and cath¬ ecolic derivatives, suitable pro-drugs are obtained by esterifica- tion of one or two hydroxy groups with pharmaceutically acceptable salts. Specific examples of pro-drugs of the compounds of formula I are acetoxy derivatives, wherein the hydroxy groups are esterified with acetic acid, and mono- or di-phn^phon^tes, wherein one or both hydroxy groups are esterified with phosphoric acid. The compounds of formula I also when transformed into pro-drugs and in particular the compounds obtained by esterification of the phen- olic hydroxy groups or of one or both the cathecolic hydroxy groups with pharmaceutically acceptable acids, as well as the pharmaceuti¬ cal compositions which contain a compound of formula I in the form of a corresponding pro-drug, and in particular which contain a compound of formula I wherein the phenolic hydroxy groups or of one or both the cathecolic hydroxy groups are esterified with pharmaceu¬ tically acceptable acids are within the scope of the present inven¬ tion. In order to better illustrate the present invention the following examples are now given. The chro atographic purifications were carried out on silica gel (230-400 mesh) columns. The mass spectra were carried out under the following conditions: chemical ionization, isobutane, positive ions. Example 1 Preparation of 2.3-dimethoxybenzenethiol A solution of n.butyl lithium in hexane (67 ml; 0.167 moles) was gently added to a cooled ( -20°C) solution of 1 ,2-dιmethoxyt.enzene (1 .2 ml; 0. 1 1 moles ) and tetramethylethylendiam e (25 ml; C. " _7 5 moles) in anhydrous tetrahydrofuran (100 ml), under nitrogen atmo¬ sphere. The mixture was stirred at room temperature for 1 hour, coolec at -_0°C and treated with recrystallized sulphur (5.36 g; 0.1.7 moles/, added in one portion. 10 The reaction mixture, after stirring at room temperature overnignt, was αυenched with aqueous potassium carbonate (100 ml) an extracted with ethyl ether (100 ml). The aqueous phase was acidified with hydrochloric acid (pH about 1 ) and re-extracted twice with ethyl acetate. 15 The collected organic layers were dried on anhydrous sodium sulphate and evaporated to afford an orange oil which was purified by flash chromatography (eluent hexane:ethyl acetate=98:2) obtaining 2,3-dι- methoxybenzenethiol (9.7 g; 51% yield) as a yellow oil. " Η-NMR (200 MHZ, CDCl 3 ): δ (ppm): 6.96-6.64 (m, 3H); 3.87-3.82 (m, 20 7H) * Mass: 171 [M+1]. Example 2 Preparation of 2-[ [ (2.3-dιmethoχyphenyl)thιolmethyl]propenoιc ac d By working according to the method described on page 47 of European __ patent application No. 0 161 218 (Ciba-Geigy AG), 2-[[(2,3-αιmeth- 2b oxyphenyl)thιo]methyl]propenoιc acid (92"/. yield) was obtameα as a yellow oil. ■■ H-NMR (200 MHz, CDCl 3 ): δ (ppm): 10.0 (bs, 1H); 7.02-6.75 ( , 3H); 6.29 and 5.72 (2bs, 2H); 3.86-3.83 (2s, 6H); 3.78 (s, 2H). Mass: 255 [M+1]. 30 Analogously the following compound was prepared. 2-[[ (2-methoxyphenyl)thio]methyl]propenoic acid having physico-chemical characteristics corresponding to those described in the European patent application No. 0 161 218. Example 3 Preparation of .4-dihvdro-7.8-dimethoχy-l (2H)-benzothiopyran-3- carboxylic acid By working according to the method described on page 47 of European patent application No. 0 161 218 (Ciba-Geigy AG), 3, -dihydro-7,8- -dimethoxy-1 (2H)-benzothiopyran-3-carboxylic acid (73 * /. yield) wa obtained as a yellow solid. ■■ H-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.80 (d, IH); 6.61 (d, 1H); 3.83 and 3.81 (2s, 6H); 3.21-2.90 (m, 5H). Mass: 255 [M+1 ]. Analogously the following compound was prepared. 3.4-dihydro-8-methoxy-l (2H)-benzothioPyran-3-carboxylic acid having physico-chemical characteristics corresponding to those described in European patent application No. 0 161 218. Example 4 Preparation of 3.4-dihvdro-7.8-dimethoxy-l (2H)-benzothiopyran-3- amine hydrochloride A solution of 3,4-dihydro-7,8-dimethoxy-l (2H)-benzothiopyran-3- carboxylic acid (9.5 g; 0.037 moles), prepared as described in example 3, in distilled t.butanol (60 ml) was stirred at room tem¬ perature, under nitrogen atmosphere. Diphenylphosphorylazide (8.9 ml; 0.041 moles) and distilled trieth¬ ylamine (5.72 ml; 0.041 moles) were added and the solution was heated under reflux for 19 hours. The formation and the disappearance of the isocyanate infrared peak (2254 cm -1 ) were used to monitor the reaction. The solvent was removed under vacuum and the residue was taken up with ethyl acetate (200 ml), washed with aqueous sodium bicaroonate, 5 followed by hydrochloric acid (2.5 * /.) and brine. The organic layers were dried on anhydrous sodium sulphate and concentrated under reduced pressure to give the N-[ [(1 , 1 -dimethyl)- ethoxy]carbonyl]amιne intermediate (13 g) as a yellow oil. This raw intermediate was dissolved in ethyl acetate saturated with 10 hydrochloric acid (100 ml) and stirred overnight to afford, after filtration. 3.4-dιhydro-7.fi- ιmpthoχy-ι r2Hi-benzothιopyran-3-amme hydrochloride (63% yield) as a white solid. Λ H-NMR (200 MHz, DMS0-d«): δ (ppm): 8.45 (bs, 3H); 6.87 (d, 1H); 6.78 (d, 1H); 3.87 and 3.79 (2s, 6H); 3.70-3.56 (m, 1H); 3.22-2.79 15 (m, 4H) . Mass: 226 [M+1 ]. By working in a similar way the following compound was prepared. 3.4-dihvdro-8-methoχy-1 (2H)-benzothioPyran-3-amιne hydrochloride white solid. 20 " Η-NMR (200 MHz, DMS0-d*): δ (ppm): 8.50 (bs, 3H); 7.09-6.70 ( , 3H); 3.79 (s, 3H); 3.72-3.57 (m, 1H); 3.24-2.88 <m, 4H). Mass: 196 [M+l]. Example 5 Resolution of ( ±)-3.4-dihvdro-8-methoxy-l (2H)-benzopyran-3-amine __ In absolute ethanol (1200 ml), (+)-3,4-dihydro-8-methoxy-l (2H)-ben- 2b zopyran-3-amine (26 g; 0.145 moles) and (S)-2-(6-methoxy-2-naphth- yDpropionic acid (33.4 g; 0.145 moles) were heated up to complete dissolution. The solution was brought to room temperature very slowly (about 24 hours) . 0 The solid obtained by filtration was crystallized twice from ethanol giving (S)-2-(6-methoxy-2-naphthyl)propιonate of (S)-3,4-dιbyαro-&- methoxy-1 (2H)-benzopyran-3-amme (11.6 g; 19.5% yield). m.p. 163-164°C e.e. 97% (HPCE) [α] D =0° (1%, methanol) By working in a similar way but using (R)-2-(6-rnethoxy-2-naρhthyi)- propiomc acid, (R)-2-(6-methoxy-2-naphthyl)propιonate of (R)-3,4- dihydro-δ-methoxy-1 (2H)-benzopyran-3-amme (22.7% yield) was ob- .p. 163-164°C e.e. 96.2% (HPCE) [α] D =0° (1%, methanol) " Η-NMR (200 MHz, CDC1 3 ): δ (ppm): 7.68-6.52 (m, 9H); 4.13-3.70 (m, 3H); 3.88 (s, 3H); 3.83 (s, 3H); 3.41-3.30 (m, 1H); 3.02-2.49 (m, 2H); 1.51 (d, 3H). By treatment of the above reported salts with a saturated solution of sodium bicarbonate and extraction with ethyl ether, the following compounds were obtained. (S)-3.4-dιhvdro-8-methoxy-l (2H)-benzopyran-3-amine hygroscopic white solid, [α]j_ = + 53° (1%, methanol) ■■ H-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.72 (m, 3H); 4.21 ( , 1H); 3.87 (m, 1H); 3.85 (s, 3H); 3.36 ( , 1H); 3.05 (dd, 1H); 2.56 (dd, IH); 1.48 (bs, 2H). (R) -3.4-dihvdro-8 -methoxy- 1 (2H)-benzopyran-3-amine hygroscopic white solid, [α]_,=-54.6° (1%, methanol) Example 6 Preparation of (R)-N-[3.4-dihvdro-8-methoχy-i (2H)-benzopyran-Ξ-yl!- propanamide Propionyl chloride (3.73 ml; 42.6 mmoles) was added to a solution of 5 (R)-3,4-dihydro-8-methoxy-1 (2H)-benzopyran-3-amme (5.6 g; 28.4 mmoles), prepared as described in example 5, and of triethylamine (5.9 ml; 42.6 mmoles) in anhydrous methylene chloride (100 ml), cooled at 0°C and under nitrogen. The reaction mixture was kept under stirring at room temperature for 02 hours. After washing with HCI 1% and with s saturated solution of sodium bicarbonate, the organic phase was dried on annydrous sodium sul¬ phate and evaporated to dryness. The resultant yellow solid was triturated in ethyl ether giving 5 (R)-N-[3,4-dihydro-8-methoxy-1 (2H)-benzopyran-3-yl]propanamide (6.2 g; 93% yield) as a white solid. m.p. 97-98°C " Η-NMR (200 MHz, CDC1 3 ): δ (ppm): 6.87-6.60 (m, 3H); 5.99 (bd, 1H); 4.52-4.41 (m, 1H); 4.29-4.05 (m, 2H>; 3.80 (s, 3H); 3.15-2.68 ( , 0 2H); 2.12 (q, 2H); 1.07 (t, 3H). By working in a similar way the following compounds were prepared. N-[3.4-dihydro-7.8-dimethoxy- (2H)-benzopyran-3-yl]propanamide white solid (100% yield). m.p. 106-107°C - " Η-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.72 (d, 1H); 6.51 (d, 1H); 5.79 (bd, 1H); 4.52-4.40 (m, 1H); 4.29-4.06 (m, 2H); 3.85 (s, 3H); 3.83 (s, 3H); 3.11-2.62 (m, 2H); 2.13 (q, 2H); 1.09 (t, 3H). N-[3.4-dihvdro-7.8-dimethoxy-l (2H)-benzothiopyran-3-yl]propanamιde white solid ( 97% yield) . m. p. 1 23-1 24°C 0 'H-NMR (200 MHZ, CDCl 3 ): δ (ppm): 6.73 (d, 1H); 6.61 (d, 1H); 5.91 (bd, 1H); 4.71-4.58 (m, 1H); 3.84-3.81 (2s, 6H); 3.19-2.74 (rr., 4H); 2.13 (q, 2H); 1.08 (t, 3H). 5 Mass: 282 [M+1 ] . N- [3.4-dihydro-8-methoxy-l (2H)-benzothiopyran-3-yl]propanamide white solid (98% yield). " Η-NMR (200 MHz, CDC1 3 ): δ (ppm): 7.03-6.62 (m, 3H); 5.95 (bd, 1H); 4.73-4.60 ( , 1H); 3.87 (s, 3H); 3.22-2.80 (m, 4H); 2.12 (q, 2H ) ; 101.08 (t, 3H). Mass: 252 TM+11. Example 7 Preparation of (R)-N-propyl-3.4-dihvdro-8-methoχy-l (2H)-benzopyran- 3-amine hydrochloride 15 Borane-dimethylsulphide (6.6 ml; 66.3 mmoles) was added to a solu¬ tion of (R)-N-[3,4-dihydro-8-methoxy-1 (2H)-benzopyran-3-yl]propan- a ide (5.2 g; 22.1 mmoles), prepared as described in example 6, in anhydrous tetrahydrofuran (100 ml), kept under nitrogen at room temperature. 20 At the end of the addition, the reaction mixture was heated under reflux for 1 hour. After cooling to room temperature, a mixture methanol:concentrated HC1=9:1 (180 ml) was added dropwise. After further heating under reflux for 1 hour, the solvent was - c evaporated and the residue was collected three times with methanol and twice with ethanol, by evaporating to dryness each time. (R)-N-propyl-3,4-dihydro-8-methoxy-1 (2H)-benzopyran-3-amine hydro¬ chloride (4.4 g; 86% yield) was obtained as a white solid, m.p. 234-236°C (dec. ) " Η-NMR (200 MHz, CDC1 3 ): δ (ppm): 10.20-9.70 (bs, 2H); 6.88-6.62 (m, 0 3H); 4.68-4.40 (m, 2H); 3.81 (s, 3H); 3.73-3.49 ( , 1H); 3.45-2.90 ( , 4H); 2.08-1.87 (m, 2H); 1.01 (t, 3H). By working in a similar way the following compounds were prepareα. 5 N-propyl-3.4-dιhvdro-7.8-dιmethoxy-1 (2H)-benzopyran-3-am ne hydro¬ chloride (89% yield) as a white solid. m.p. 216-218°C ■■ H-NMR (200 MHz, CDC1 3 ): δ (ppm): 10.10-9.75 (bs, 2H); 6.72 (c, 1H); 6.50 (d, 1H); 4.69-4.33 (m, 2H); 3.81 (s, 6H); 3.70-3.50 (m, IH); Q 3.36-2.85 (m, 4H); 2.07-1.88 (m, 2H); 1.01 (t, 3H). N-propyl- . -d hvdro-7 l 8-dιmethoχy-1 (2H)-benzoth opyrari-l-armnp hydrochloride (99% yield) as a white solid. m.p. 229-231°C (dec. ) " "H-NMR (200 MHz, DMS0-d A ): δ (ppm): 9.30 (m, 2H); 6.88 (d, 1H); 6.79 5 (d, 1H); 3.78 and 3.69 (2s, 6H); 3.70-3.52 (m, 1H); 3.42-2.89 ( , 6H); 1.79-1.59 (m, 2H); 0.92 (t, 3H). Mass: 304 [M+1]. N-propyl-3.4-dιhvdro-8-methoχy-1 (2H)-benzothιopyran-3-amιne hydro¬ chloride (98% yield) as a white solid. 0 η H-NMR (200 MHz, DMS0-d_): δ (ppm): 9.38 (m, 2H); 7.09-6.71 (tn, 2H); 3.79 (s, 3H); 3.72-3.55 (tn, 1H); 3.40-2.90 ( , 6H); 1.79-1.59 (tn, 2H); 0.92 (t, 3H). Mass: 274 [M+1 ]. Example 8 Preparation of 6-[ (4-methoχyphenyl)acetylamιno]hexanoιc acid b N,N-dιmethylformamιde (0.1 ml) and thionyl chloride (3.5 g; 29.4 mmoles) were added under stirring at room temperature to a solution of 4-methoxyphenylacetιc acid (3.3 g; 19.8 moles) in methylene chloride (50 ml) . After 1 hour, the solvent was evaporated under reduced pressure 0 obtaining an oil which was dissolved in methylene chloride (6 ml). The resultant solution was added dropwise, contemporaneously with a solution of sodium hydroxide 4N (5 ml) and under vigorous stirring, 5 to a solution of 6-aminohexanoic acid (2.5 g; 19.1 mmoles) and sodium hydroxide (0.8 g; 20 mmoles) in water (10 ml). The reaction mixture was kept under stirring at room temperature for 3 hours. The phases were separated and the aqueous phase was washed with 0 methylene chloride (10 ml), acidified with hydrochloric acid 37% up to pH 1 and extracted with methylene chloride (15 ml). After drying the resultant organic phase on anhydrous sodium sul¬ phate, the solvent was evaporated under reduced pressure. The solid residue was triturated in ethyl ether obtaining after 5 filtration 6-[ (4-methoxyphenyl)acetylamino]hexanoic acid (4.4 g; 79.5% yield) as a white solid. m.p. 93-94°C (from ethyl acetate) " Η-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.18-6.81 (m, 4H); 5.43 (bt, 1H); 3.79 (s, 3H); 3.49 (s, 2H); 3.17 ( , 2H); 2.28 (t, 2H); 1.66-1.16 0 (CT, 6H). Mass: 280 [M+1 ]. By working in a similar way the following compound was prepared. 6-[ ( 3.4-dimethoxyphenyl)acetylamino]hexanoic acid 58% yield - m.p. 75-76°C " Η-NMR (200 MHz, DMS0-d 4 ): δ (ppm): 7.94 (bt, 1H); 6.89-6.70 (m, 3H); 3.70 (s, 3H); 3.69 (s, 3H); 3.28 (s, 2H); 2.99 (m, 2H); 2.17 (t, 2H); 1.51-1.12 (m, 6H). Mass: 310 [M+1 ]. Example 9 0 Preparation of 6-[ (2-methoχy-1 ^-benzothiazol-ά-vDacetylam no 1 ,- hexanoic acid Potassium thiocyanate (257 g; 2.64 moles) was added to a solution of 54-amιnophenylacetic acid (200 g; 1.32 moles) in water (1000 ml) and concentrated hydrochloric acid (130 g; 1.32 moles), under stirring at 50°C. The reaction mixture was heated at 100°C for 16 hours, then cooled to 0°C and filtered. 10 Absolute ethanol (600 ml) was added to the solid and the mixture was heated υndpr rpflux for 1 hour After cooling to 0°C, filtering and drying the solid under vacuum at 60°C, 4-(2-carboxyethyl)phenylthiourea (173 g) was obtained as a solid. 15 1 H-NMR (200 MHz, DMS0-d A ): δ (ppm): 12.30 (bs, 1H); 9.64 (s, 1H); 7.70-7.20 (bs, 2H); 7.35-7.16 (m, 4H) ; 3.52 (s, 2H). Mass: 211 [M+1]. Bromine (261 g; 1.64 moles) was slowly added to a suspension of 4-(2-carboxyethyl)phenylthiourea (172 g; 0.82 moles) in chloroform 20 (1.7 1), under stirring at 0°C. After 30 minutes the reaction mixture was heated under reflux for " hour, then cooled at 0°C and filtered. The solid was washed with acetone and dissolved in water (2.5 1); a 10.8N aqueous sodium hydroxide solution (67 ml) was added under -- stirring at room temperature. After 1 hour, the solid was filtered and washed with water. After drying under vacuum at 60°C, 2-amino-6-(2-carboxyethyl)-l ,3- benzothiazole (129 g) was obtained. " Η-NMR (200 MHz, DMS0-d*): δ (ppm): 12.45-12.11 (bs, 1H); 7.52 (d, 1H); 7.41 (bs, 2H); 7.25 (d, 1H); 7.08 (dd, 1H); 3.55 (s, 2H). 30 Mass: 209 [M+1 ]. A solution of sodium nitrite (104 g; 1.5 moles) in water (155 ml) was added dropwise in 2 hours, under stirring at 0°C, to a solution 5 of 2-amino-6-(2-carboxyethyl)-1 ,3-benzothiazole (104 g; 0.5 moles) in 85% phosphoric acid (1145 ml). After 1 hour the mixture was added in 1 hour to a solution of CuS0_-5H 2 0 (500 g; 2.0 moles) and sodium chloride (584 g; 10.0 moles) in water (2.0 1), under stirring at 0°C. 10The temperature of the reaction mixture was let spontaneously arise up to the room value overnight; after filtration, th solid ua washed with water and dried under vacuum at 60°C obtaining 2-chloro- -6-(2-carboxyethyl)-l ,3-benzothiazole (104 g). "• H-NMR (200 MHz, DMS0-d Λ ): δ (ppm): 12.42 (bs, 1H); 7.99 (d, IH); 157.89 (d, 1H); 7.44 (dd, 1H); 3.73 (S, 2H). Mass: 228 [M+1]. Metallic sodium (17.8 g) was added portionwise to methanol (800 ml) under stirring. After complete dissolution, 2-chloro-6-(2-carboxy- ethyl)-l ,3-benzothiazole (80 g; 0.35 moles) was added and the reac- 0 tion mixture was heated under reflux for 3 hours. The solvent was evaporated and water (640 ml), methanol (160 ml) and, under stirring at room temperature, concentrated hydrochloric acid (44 ml) were added to the residue. After cooling at 0°C and filtering, the solid was dried under vacuum c at 60°C obtaining 2-methoxy-6-(2-carboxyethyl)-l ,3-benzothiazole (71 _.b g) as a beige solid. n H-NMR (200 MHz, DMS0-d«): δ (ppm): 12.37 (s, 1H); 7.76 (d, 1H); 7.61 (d, 1H); 7.28 (dd, 1H); 4.13 (s, 3H); 3.64 (5, 2H). Mass: 224 [M+1]. N,N'-dicyclohexylcarbodiimide (1.85 g; 9.0 mmoles) was added to a 30 solution of 2-methoxy-6-(2-carboxyethyl)-l ,3-benzothiazole (2.0 g; 9.0 mmoles) and N-hydroxysuccinimide (1.0 g; 9.0 mmoles) in dioxane (15 ml), under stirring at room temperature. 5 After 1 hour ethyl ether (15 ml) was added and the reaction mixture was filtered. The solution was evaporated to dryness and the resultant residue was dissolved in dioxane (10 ml) and N,N-dιmethylformamide (5 ml). The resultant solution was added, under stirring at room temperat- 10 ure, to a solution of 6-amιnohexanoιc acid (1.2 g; 9.0 mmoles) and dicvclohexylamine (1.6 g; 9.0 mmoles) in water '6 m l) and ethanol (6 ml). After 1 hour, the solvents were evaporated under reduced pressure, water (20 ml) and, under stirring, a solution of potassium hydroge- 15 nosulphate (1.3 g; 9.6 mmoles) in water (6 ml) were added. After 30 minutes the precipitate was filtered and dried under vacuum at 60°C obtaining 6-[ (2-methoxy-l ,3-benzothiazol-6-yl)acetylam o]- hexanoic acid (2.8 g) as a white solid. " Η-NMR (200 MHz, DMSO-d*): δ (ppm): 8.03 (t, IH); 7.71 (d, IH); 7.58 0 <d, IH); 7.26 (dd, 1H); 4.13 (s, 3H); 3.44 (s, 2H); 3.08-2.94 (m, 2H); 2.16 (t, 2H); 1.56-1.12 (m, 6H). Mass: 337 [M+1 ]. Example 10 Preparation of (R)-N-propyl-N-[6-[ (4-methoχyphenyl)acetylamino]-l - ? _ -oxohexyl]-3.4-dihvdro-8-methoxy-3-[1 (2H)-benzopyrany lamine Thionyl chloride (3.57 g; 30 mmoles) was added to a suspension of 6-[ (4-methoxyphenyl)acetylamino]hexanoιc acid (5.6 g; 20 mmoles), prepared as described in example 8, in anhydrous methylene chloride (60 ml), under nitrogen at room temperature. The solution was kept for 1 hour at room temperature and the solvent 30 was evaporated under reduced pressure obtaining a thick oil (2.53 g) which was dissolved in anhydrous methylene chloride (10 ml). The resultant solution was added dropwise to a solution obtained by adding triethylamine (1.77 g; 17.5 mmoles), under stirring at room temperature, to a suspension of (R)-N-propyl-3,4-dihydro-8-methoxy- -1 (2H)-benzopyran-3-amine hydrochloride (1.5 g; 5.82 mmoles), pre¬ pared as described in example 7, in anhydrous methylene chloride (50 ml). The reaction mixture was kept under stirring at room temperature for 2 hours. After washing with HCI 1% and with a saturated solution of sodium bicarbonate, the organic phase was dried on anhydrous sodium sul¬ phate and evaporated to dryness under reduced pressure obtaining (R) -N-propyl-N-[6-[ (4-methoxyphenyl)acetylamino]-l -oxohexyl]-3,4-di- hydro-8-methoxy-3-[1 (2H)-benzopyranyl]amine (2.8 g; 100% yield) as a yellow solid. "• H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.18-6.61 (m, 7H); 5.60-5.40 (m, 1H); 4.40-3.88 (m, 3H); 3.86 (s, 3H); 3.78 (s, 3H); 3.48 (s, 2H); 3.33-2.79 (m, 6H); 2.43-2.22 (m, 2H); 1.69-1.20 (m, 8H); 0.85 (t, 3H). Mass: 483 [M+1]. By working in a similar way the following compounds were prepared: (R)-N-PΓOPVI-N-[6-[ (3.4-dimethoχyphenyl)acetylamino1-1 -oxohexyll- 3. -dihydro-8-methoχy-3-[1 (2H)-benzopyranyllamine (94.2% yield) as a thick oil. ■• H-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.90-6.61 (m, 6H); 5.67-5.51 (m, 1H); 4.45-3.90 (m, 3H); 3.85 (s, 9H); 3.49 (s, 2H) ; 3.33-2.80 (m, 6H); 2.39-2.22 ( , 2H); 2.15-1.20 (m, 8H); 0.85 (t, 3H). Mass: 513 [M+1]. N-PΓOPV1-N-[6-Γ (3.4-dimethoχyphenyl)acetylamino1-1 -oxohexyl " i-3.4-di- hydro-7.8-dimethoxy-3-f1 (2H)-benzopyranyllamine (98.3% yield) as a thick oil. " -H-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.85-6.44 (m, 5H); 5.62-5.50 (m, 1H); 4.49-3.92 ( , 3H); 3.86 and 3.83 and 3.81 (3s, 12H); 3.49 (s, 2H); 3.27-2.64 (m, 6H); 2.39-2.23 (m, 2H); 1.70-1.20 (m, 8H); 0.85 (t, 3H). Mass: 543 [M+1 ]. N-propy1-N-[6-[ (4-methoxyphenv1)acetylamino1-1 -oxohexyl1-3.4-di- hvdro-7.8-Hirnρthnvy-?- i t2H)-benzopyranyllamine (100% yield) as a waxy solid. "• H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.15-6.45 (m, 6H); 5.60-5.48 ( , 1H); 4.49-3.89 (m, 3H); 3.83 and 3.81 and 3.78 (3s, 9H); 3.48 (s, 2H); 3.25-2.75 ( , 6H); 2.42-2.22 (m, 2H); 1.68-1.20 (m, 8H); 0.85 (t, 3H). Mass: 513 [M+1]. N-propyl-N-[6-[ (2-methoχy-6-benzothiazolyl)acetylamino]-i -oxohexy l- 3.4-dihvdro-7.8-dimethoxy-3-[1 (2H)-benzopyranyllamine (69% yield) as a thick oil. " Η-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.61-7.15 (m, 3H); 6.76-6.42 ( , 2H); 5.90-5.79 ( , 1H); 4.43-3.85 (m, 3H); 4.15 (s, 3H); 3.82 and 3.78 (2s, 6H); 3.55 (s, 2H); 3.25-2.73 ( , 6H); 2.36-2.20 (m, 2H); 1.65-1.20 (m, 8H); 0.83 (t, 3H). Mass: 570 [M+1 ]. (R)-N-propyl-N-f6-[ (2-methoxy-6-benzothiazolyl)acetylaminoA -oxohe¬ xyl!-3.4-dihvdro-8-methoxy-3-[1 (2H)-benzopyranyllamine (54% yield) as a sticky yellow oil. "• H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.64-7.18 (m, 3H); 6.90-6.60 ( , 3H); 5.71-5.63 (m, 1H); 4.40-3.91 (m, 3H); 4.18 (s, 3H); 3.86 and 3.84 (2s, 3H); 3.59 (s, 2H); 3.34-2.63 (m, 6H); 2.38-2.22 ( , 2H); 1.70-1.22 (m, 8H); 0.94-0.80 ( , 3H). Mass: 540 [M+1 ] . 5 N-propyl-N-[6-[ (3.4-dimethoχyphenyl)acetylaminol-l -oxohexyll-3.4-di- hvdro-8-methoxy-3-[1 (2H)-benzothiopyranyllamine (94% yield) as a yellow amorphous solid. " Η-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.05-6.61 (m, 6H); 5.68-5.55 ( , 1H); 4.50-4.19 ( , 1H); 3.84 (m, 9H); 3.48 (s, 2H) ; 3.42-2.77 ( , 0 8H); 2.34-2.24 ( , 2H); 1.68-1.24 (m, 8H); 0.95-0.82 ( , 3H). Mass: 529 [M+1 ] . N-propyI-N-[6-[ (4-methoxyphenyl)acetylaminol-i -oxohexyl]-3.4-dι- hvdro-8-methoxy-3-r1 (2H)-benzothioPyranyllamine (100% yield) as a yellow oil. 5 " "H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.18-6.81 (m, 4H); 7.05-6.61 (m, 3H); 5.59-5.49 (m, 1H); 4.50-4.19 (m, 1H); 3.85-3.76 (m, 6H); 3.48-2.76 (m, 10H); 2.35-2.23 (m, 2H); 1.70-1.22 (m, 8H); 0.95-0.84 (m, 3H) . Mass: 499 [M+1]. 0 N-PΓOPV1-N-[6-[ ( 2-methoχy-6-benzothiazolyl)acetylaminol-l -oxohexyl]- 3. -dihvdro-8-methoχy-3-[1 (2H)-benzothiopyranyllamine (64% yield) as a white solid. " "H-NMR (200 MHz, CDC1 3 ): δ (ppm): 7.62-7.18 (m, 3H); 7.04-6.59 (m, 3H); 6.90-6.77 (m, 1H); 4.51-4.22 (m, IH); 4.15 (s, 3H); 3.74 and - 3.71 (2s, 3H); 3.58 (s, 2H); 3.48-2.74 (m, 8H); 2.33-2.20 ( , 2H); 1.68-1.18 (m, 8H); 0.93-0.82 (m, 3H). Mass: 556 [M+1 ]. N-propyl-N-[6-[ (2-methoχy-6-benzothiazolyl)acetylamino1-1-oxohexyl]- 3. -dihvdro-7.8-dimethoχy-3-[1 (2H)-benzothiopyranyl1amine (100% yield) as an orange oil. 1 H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.64-7.18 (m, 3H); 6.77-6.55 ( , 2H); 5.76-5.55 (m, IH); 4.47-4.21 (m, 1H); 4.17 (s, 3H); 3.82-3.79 (m, 6H); 3.58 (s, 2H); 3.43-2.68 (m, 8H); 2.41-2.22 (m, 2H); 1.67-1 5.19 (m, 8H); 0.95-0.83 (m, 3H). Mass: 586 [M+1]. N-propyl-N-[6-[ (3.4-dimethoχyphenyl)acetylamino]- 1 -oxohexylI-3.4-di- hvdro-7.8-dimethoxy- -[ ( 2H)-benzothiopyrany11amine (86% yield) as a yellow oil. 10 'H-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.83-6.55 (m, 5H); 5.66-5.56 (m, IH); 4.49-4.16 (m, 1H); T. 4-^. 9 (rp, 12H); 3.47 (s, 2H); 3.43-2.69 (m, 8H); 2.34-2.23 (m, 2H) ; 1.67-1.20 (m, 8H); 0.93-0.83 (m, 3H). Mass: 559 [M+1 ]. N-propyl-N-[6-[ (4-methoχyphenyl)acetylaminol-l -oxohexyl1-3. -di- 1 hydro-7.8-dimethoχy-3-[l ( 2H)-benzothiopyrany1lamine (94% yield) as a yellow oil. "■ H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.18-6.82 (m, 4H); 6.81-6.55 (m, 2H); 5.63-5.53 (m, IH); 4.50-4.18 (m, 1H); 3.83-3.76 (m, 9H); 3.48 (s, 2H); 3.47-2.69 ( , 8H); 2.36-2.23 ( , 2H); 1.70-1.20 (m, 8H); 200.95-0.82 (m, 3H) . Mass: 529 [M+1 ]. Example 11 Preparation of N-propyl-N-T6-[ (2.3-dihvdro-2-oxo-6-benzothiazolyl)- acetylamino]-l -oxohexyl!-3.4-dihvdro-8-methoxy-3- 11 (2H)-benzothio- ? - pyranyllamine Concentrated hydrochloric acid (37%; 0.3 mi; 0.0036 moles) was slowly added to a stirred solution of N-propyl-N-[6-[ (2-methoxy-6- benzothiazolyl)acetyla ino]-1 -oxohexyl]-3,4-dihydro-8-methoxy-3- [1 (2H)-benzothiopyrany1]amine (1 g; 0.0018 moles), prepared as described in example 10, in dioxane (10 ml). 30 The resulting mixture was stirred for 1.5 hours, at room temperat¬ ure, concentrated under reduced pressure, taken up with tetrahydro¬ furan (50 ml) and washed with aqueous sodium bicarbonate. 5 The organic layer was dried on anhydrous sodium sulphate and evapo¬ rated to afford N-propyl-N-[6-[ (2,3-dihydro-2-oxo-6-benzothiazolyl)- acetylamino]-1 -oxohexyl]-3,4-dihydro-8-methoxy-3-[i (2H)-benzothio- pyranyl]amine (1 g; 100% yield) as a white foam. 1 H-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.21-6.60 (m, 6H); 6.31-6.15 (m, 10 IH); 4.56-4.19 (m, 1H); 3.85 and 3.82 (2s, 3H); 3.49 (s, 2H); 3.48-2.76 (m, 8H ) ; 2.38-2.25 ( , 2H); 1.70-1.20 ( , 8H); 0.95-0.82 (m, 3H). Mass: 542 [M+1 ] . By working in a similar way the following compounds were prepared. 15 N-propyl-N-[6-[ (2.3-dihvdro-2-oxo-6-benzothiazolyl)acetylamino]-i - -oxohexyl1-3.4-dihvdro-7.8-dimethoχy-3-[1 (2H)-benzothiopyranyllamine (77% yield) as a brown foam. "• H-NMR (200 MHz, CDC1 3 , 60°C): δ (ppm): 7.08-6.53 (m, 5H); 6.07 (bs, 1H); 4.43-4.13 (m, 1H); 3.83-3.75 (m, 6H); 3.39 (bs, 2H); 3.31-2.69 20 <m, ΘH); 2.27-2.20 (m, 2H); 1.65-1.17 (m, 8H); 0.92-0.82 (m, 3H). Mass: 572 [M+1]. N-propyl-N-[6-[ (2.3-dihydro-2-oxo-6-benzothiazolyl)acetylamino " i-' -o- xohexyll-3.4-dihvdro-7.8-dimethoxy-3-[l (2H)-benzopyranyllamine (98% yield) as a white solid. -- η H-NMR (200 MHz, CDC1 3 ): δ (ppm): 7.11-6.47 (m, 5H); 6.10-5.98 (m, _o 1H); 4.53-3.90 (m, 3H); 3.85 and 3.81 (2s, 6H); 3.52 (s, 2H); 3.30-2.78 (m, 6H); 2.39-2.28 (m, 2H); 2.00-1.20 (m, 8H); 0.86 (t, 3H). Mass: 556 [M+1]. (R)-N-propyl-N-[6-[ (2.3-dihydro-2-oxo-6-benzothiazolyl)acetylamino]- 30 1 -oxohexyl!-3.4-dihvdro-8-methoxy-3- ϊ1 (2H)-benzopyranyl!amine ( 100% yield) as a white solid. "Η-NMR (200 MHz, CDCl 3 ): δ (ppm): 7.25-6.63 (m, 6H); 6.03-5.9" (m, 5 lH); 4.57-4.13 (m, 3H); 3.87 (s, 3H); 3.52 (s, 2H); 3.31-2.82 ( , 6H); 2.39-2.28 (m, 2H); 1.92-1.20 (m, 8H); 0.86 (t, 3H). Mass: 526 [M+1 ]. Example 12 Preparation of (R)-N-propyl-N-[6-[2-(4-methoχyphenyl)ethylaminoIhex- 10 yl1-3.4-dihydro-8-methoxy-3-[1 (2H)-benzopyranyllamine dihydrochlo¬ ride By working in a way similar to that described in example 7 but using (R)-N-propyl-N-[6-[ ( -methoxyphenyl)acetylamino]-1 -oxohexyl]-3,4-di- hydro-8-methoxy-3-[1 (2H)-benzopyranyl]amine (3.36 g; 6.99 mmoles), 15 prepared as described in example 10, anhydrous tetrahydrofuran (100 ml) and borane-dimethylsulphide (4.5 ml; 45.5 mmoles), (R)-N-propyl- N-[ -[2-(4-methoxyphenyl)ethylamino]hexy1]-3,4-dihydro-8-methoxy-3- [1 (2H)-benzopyranyllamine dihydrochloride (3.63 g; 98% yield) was obtained as an amorphous solid. 0 "• H-NMR (200 MHz, D a 0): δ (ppm): 7.15-6.67 (m, 7H); 4.45-4.19 (m, 2H); 3.90-3.82 (m, IH); 3.69 (s, 3H); 3.66 (s, 3H); 3.51-2.77 ( , 12H); 1.68-1.15 ( , 10H); 0.80 (t, 3H). Mass: 455 [M+1 ]. Analogously the following compounds were prepared. __ (R)-N-propyl-N-[6-[2-(3.4-dimethoxyphenyl)ethylamιnolheχyl]-3,4-di- 2b hvdro-8-methoxy-3-[1 (2H)-benzopyranyl!amine dihydrochloride (94% yield) as a white amorphous solid. "• H-NMR (200 MHz, DMS0-d 4 ): δ (ppm): 11.0 (bs, 1H); 9.09 (bs, 2H); 6.90-6.70 (m, 6H); 4.51-4.23 ( , 2H); 3.91-3.78 (m, 1H); 3.75 and 3.73 and 3.71 (3s, 9H); 3.70-2.80 (m, 12H); 1.85-1.25 (m, 10H); 0.90 30 (t, 3H). Mass: 558 [M+1 ]. N-propy I-N- [ - [ 2- ( -methoxyphenyl ) ethy lamino Ihexy 11 -3. -dihvdro-7.8- 5 dimethoχy-3-[l (2H) -benzopyranyl lamine dihydrochloride (100% yield) as an amorphous solid. π H-NMR (200 MHz, D 2 0): δ (ppm): 7.12-6.58 ( , 6H); 4.48-4.20 ( , 2H); 3.90-3.80 (m, 1H); 3.67 and 3.65 and 3.62 (3s, 9H); 3.25-2.72 (m, 12H); 1.67-1.12 ( , 10H); 0.79 (t, 3H). 10 Mass: 485 [M+1 ] . N- p ro p yl-N-f6-[?-(3. -dimethoχyphenyl ) ethy1amin I exy11- .^- - hydro-7.8-dimethoxy-3-[1 (2H)-benzopyranyl!amine dihydrochloride (95% yield) as an amorphous solid. "• H-NMR (200 MHz, CDCl 3 ): δ (ppm): 6.71-6.42 (m, 5H); 4.40-3.82 ( , 15 2H); 3.85 and 3.84 and 3.82 and 3.81 (4s, 12H); 3.19-3.02 (m, 1H); 2.88-2.42 ( , 12H); 1.60-1.22 (m, 10H); 0.84 (t, 3H). Mass: 515 [M+1]. N-propy 1-N- [ 6- [ 2- ( -methoχyphenyl ) ethy lamino Ihexy 11 -3.4-dihvdro-8- methoxy-3-[i (2H)-benzothiopyranyn amine dihydrochloride (100% yield) 20 as a white foam. "• H-NMR (200 MHz, CDC1 3 ): δ (ppm): 11.40 (bs, 1H); 9.70 (bs, 2H); 7.20-6.63 (m, 7H); 4.05-3.87 (m, IH); 3.82 and 3.75 (2s, 6H); 3.50-2.85 (m, 14H); 2.05-1.40 (m, 10H); 1.01 (bt, 3H). Mass: 471 [M+1]. ? - N-propyl-N-[6-[2-(3.4-dimethoxyphenyl)ethylaminoIhexy11-3. -di- hvdro-8-methoχy-3-r1 (2H)-benzothiopyranyllamine dihydrochloride (90% yield) as an amorphous solid. "• H-NMR (200 MHz, DMS0-d*): δ (ppm): 10.98 (bs, 1H); 9.15 (bs, 2H); 7.10-6.71 (m, 6H); 3.90-3.70 (m, 1H); 3.80 and 3.74 and 3.71 (3s, 9H); 3.60-2.70 (m, 14H); 1.90-1.25 ( , 10H); 0.91 (t, 3H) . 30 Mass: 501 [M+1]. N-propy1-N-[6-[2-(2.3-dihydro-2-oxo-6-benzothiazoiyl)ethylamino ' :he- xyl]-3.4-dihydro-8-methoxy-3-[l (2H)-benzothiopyranyllamine dihvdro- 5chloride (98% yield) as a white solid. 1 H-NMR (200 MHz, DMSO-d * ): δ (ppm): 11.99 (s, IH); 11.03 (bs, 1H); 9.20 (bs, 2H); 7.47-7.06 (tn, 3H); 6.88 (d, iH); 6.80 (d, IH); 4.05-3.83 ( , 1H); 3.76 and 3.68 (2s, 6H); 3.49-2.78 (m, 14H); 1.85-1.28 (m, 10H); 0.98-0.84 (m, 3H). 10Mass: 514 [M+1 ]. N-propyl-N-[6-[2-( 3 ,4-Him p thoxyphenyl ~>gthyl.gminnlhpyyl ]-3.4-di- hvdro-7.8-dimethoxy-3-[1 (2H)-benzothiopyranyl1amine dihydrochloride (100% yield) as a white solid. 1 H-NMR (200 MHz, CDCl 3 ): δ (ppm): 11.3 (bs, 1H); 9.67 (bs, 2H); 156.88-6.59 (m, 5H>; 3.97-3.84 (m, 1H); 3.82 and 3.81 and 3.79 and 3.78 (4s, 12H); 2.45-2.88 (m, 14H); 2.05-1.46 (m, 10H); 1.04-0.96 ( , 3H). Mass: 531 [M+1]. N-propyl-N-[6-[2-( -methoχyphenyl)ethylaminolhexyl1-3. -dihydro-7.8- 20 dimethoxy-3-f1 (2H)-benzothiopyranyllamine dihydrochloride (95% yield) as a white amorphous solid. "• H-NMR (200 MHz, CDCl 3 ): δ (ppm): 11.3 (bs, 1H); 9.60 (bs, 2H); 7.64-7.15 ( , 4H); 6.87-6.58 (m, 2H) ; 3.93-3.77 (m, 1H); 3.78 and 3.76 and 3.72 (3s, 9H); 2.86-2.46 (m, 14H); 1.99-1.39 (tn, 10H); „- 1.02-0.95 (m, 3H). _b Mass: 501 [M+1]. N-propyl-N-[6-[2-(2.3-dihvdro-2-oxo-6-benzothiazolyl)ethylaminoIhe- xyl1-3.4-dihvdro-7.8-dimethoxy-3-[1 (2H)-benzothiopyranyllamine dihydrochloride (100% yield) as a white solid. 1 H-NMR ( 200 MHz , DMSO-d* ) : δ ( ppm ) : 1 1 . 99 ( s , I H ) ; 1 1 .03 ( bs , 1 H ) ; 30 9.20 (bs, 2H); 7.47-7.06 ( , 3H); 6.88 (d,1H); 6.80 (d, IH); 4.05-3 .83 (m, 1H); 3.76 and 3.68 (2s, 6H); 3.49-2.78 (m, 14H); 1.85-1.28 (m, 10H); 0.98-0.84 ( , 3H). 5 Mass: 544 [M+1 ]. N-proPyl-N-[6-[2-(2.3-dihydro-2-oxo-6-benzothiazolyl)ethylaminolhe- xyll-3. -dihvdro-7.8-dimethoxy-3-f1 (2H)-benzopyranyl1amine dihydro¬ chloride (100% yield) as a white solid. 1 H-NMR (200 MHz, D a 0): δ (ppm): 7.29-7.00 (m, 3H); 6.78 (d,1H); 6.58 10 (d, 1H); 4.46-4.18 (m, 2H); 3.88-3.78 (m, 1H); 3.65 and 3.60 (2s, 6H): 3.22-2.78 (m. 12H): 1.67-1.10 (m, 10H); 0.77 (t, 3H). Mass: 528 [M+1 ]. N- p ropyl-N-[6-[2-(2.3-dihvdro-2-oxo-6-benzothiazolyl)ethylaminoIhe- xyll-3.4-dihydro-8-methoxy-3-[1 (2H)-benzopyranyllamine dihydrochlo- 15 ride (100% yield) as a white solid. "• H-NMR (200 MHz, D 2 0): δ (ppm): 7.22-6.61 (m, 6H); 4.39-4.11 (m, 2H); 3.86-3.76 (m, 1H); 3.64 (s, 3H); 3.24-2.80 ( , 12H); 1.65-1.12 (tn, 10H); 0.77 (t, 3H). Mass: 498 [M+1 ]. 20 Example 13 Preparation of (R)-N-propyl-N-r6-r2-t -hvdroχyphenyl)ethylaminolhex- yll-3.4-dihvdro-8-hvdroxy-3-[1 (2H)-benzopyranyl!amine dihvdrobromide (Compound 1 ) A solution of (R)-N-propyl-N-[6-[2-( -methoxyphenyl)ethylamino]hex- „_ yl]-3,4-dihydro-8-methoxy-3-[l (2H)-benzopyranyl]amine dihydrochlo- 25 ride (3.6 g; 6.9 mmoles), prepared as described in example 12, in hydrobromic acid 48% ( 25 ml) was kept under reflux for 3 hours under nitrogen. The mixture was concentrated under reduced pressure, collecting several times with absolute ethanol and then with ethanol saturated 30 with HC I . After evaporation to dryness, the crude (3.94 g) was purified by chromatography (eluent (CH 2 Cl 2 :CH 3 0H:HC00H:H a 0=80:20:2:2) obtaining Compound 1 (1.89 g; 47% yield) as an amorphous beige solid. "• H-NMR (200 MHz, D 2 0): δ (ppm): 7.07-6.60 (m, 7H) ; 4.48-4.20 (m, 2H); 3.92-3.83 (m, 1H); 3.31-2.74 (m, 12H); 1.69-1.14 ( , 10H); 0.80 (t, 3H). Mass: 427 [M+1 ]. By working in a similar way the following compounds were prepared. (R) -N-propyl-N-r -[2-(3.4-dihvdroxyphpnyl )pthylamino!hpyyl 1-3 , -di - hydro-8-hvdroχy-3-[i (2H)-benzopyranyl!amine dihvdrobromide (Compound 2) as a brown amorphous solid (73% yield) "• H-NMR (200 MHz, D a 0): δ (ppm): 6.79-6.56 ( , 6H); 4.48-4.20 (m, 2H); 3.92-3.83 (m, IH); 3.31-2.70 (m, 12H); 1.70-1.14 (m, 10H); 0.80 (t, 3H). Mass: 443 [M+1 ]. N-propyl-N-[6-[2-(3.4-dihvdroχyphenyl)ethylamino!hexyl1-3.4-di- hvdro-7.8-dihydroxy-3-[1 (2H)-benzopyranyllamine dihvdrobromide (Compound 3) as a beige amorphous solid (70% yield) "• H-NMR (200 MHz, D a 0): δ (ppm): 6.74 (d, IH); 6.68 (d, 1H); 6.59 (dd, IH); 6.50 (d, 1H); 6.43 (d, 1H); 4.47-4.17 ( , 2H); 3.87-3.78 ( , 1H); 3.22-2.69 (m, 12H); 1.68-1.11 (m, 10H); 0.80 (t, 3H). Mass: 459 [M+1]. N-propyl-N-f6-[2-(3. -dihvdroxyphenyl) thylaminoIhexyl1-3.4-dihvdro- 8-hvdroxy-3-π (2H)-benzothiopyrany1lamine dihydrobromide (Compound 4) as a light brown solid (84% yield) "• H-NMR (200 MHz, D a 0): δ (ppm): 6.92-6.53 (m, 6H); 3.98-3.86 (m, IH); 3.14-3.01 (tn, 10H); 2.87-2.79 (tn, 2H); 2.74-2.67 <m, 2H ) ; 1.68-1.19 ( , 10H); 0.79 (t, 3H) . Mass: 459 [M+1]. N-propyl-N-[6-[2-(4-hydroxyphenyl)ethylaminolhexyl1-3.4-dihvdro-8- hydroxy-3-[1 (2H)-benzothiopyranyllamine dihvdrobromide (Compound 5) as a white solid ( 33'/. yield) "• H-NMR (200 MHz, D 2 0): δ (ppm): 7.05-6.66 (m, 4H); 6.92-6.60 (tn, 3H); 3.96-3.83 (m, 1H); 3.12-2.71 (m, 14H); 1.68-1.21 (tn, 10H); 0.80 (t, 3H). Mass: 443 [M+1 ]. N-PΓQPVI-N-[6-[2-(2.3-dihydro-2-oxo-6-benzothiazolyl)ethylaminoIhe¬ xy1!~3. ~dihydro-7.8-dihydroχy-3-[1 (2H^-benzot iopyranyl1 mine dihydrochloride (Compound 6) as a white foam (49% yield). "• H-NMR (200 MHz, D a 0): δ (ppm): 7.26-6.98 (m, 3H); 6.56-6.47 (m, 2H); 3.92-3.79 ( , 1H); 3.17-2.81 (m, 14H); 1.64-1.16 (m, 10H); 0.78 (t, 3H). Mass: 515 [M+1]. N-PΓOPVI-N-[6-[2-(2.3-dihvdro-2-oxo-6-benzothiazolyl)ethylaminoIhe¬ xy11-3. -dihvdro-8-hydroχy-3-[1 (2H)-benzothiopyranyllamine dihydro¬ chloride (Compound 7) as a white solid (30% yield). "• H-NMR (200 MHz, DMS0-d*): δ (ppm): 11.89 (bs, 1H); 10.28 (bs, 1H); 9.98 (bs, 1H); 8.75 (bs, 2H); 7.47-6.59 ( , 6H); 3.95-3.70 (bm, 1H); 3.37-2.83 (m, 14H); 1.71-1.33 ( , 10H); 0.91 (t, 3H). Mass: 500 [M+1 ]. N-PΓQPVI-N-[6-[2-(3.4-dihvdroxyphenyl)ethylaminoIhexy11-3■ -di- hvdro-7.8-dihydroxy-3-f1 (2H)-benzothiopyranyllamine dihvdrobromide (Compound 8) as an amorphous solid (45% yield) "• H-NMR (200 MHz, D 2 0): δ (ppm): 6.73-6.54 (m, 5H); 3.96-3.83 (m, 1H); 3.13-2.67 (m, 14H); 1.68-1.15 (m, 10H); 0.79 (t, 3H). Mass: 475 [M+1 ]. N-propyl-N-[6-[2-(4-hvdroxyphenyl) thylaminoIhexy11-3. -dihvdro-7.8- dihvdroxy-3-f1 (2H)-benzothiopyranyllamine dihvdrobromide (Compound 9) as an amorphous solid (42% yield) "• H-NMR (200 MHz, D a 0): δ (ppm): 7.05-6.69 ( , 4H); 6.59-6.51 (m, 2H); 3.96-3.83 (m, 1H); 3.20-2.73 (m, 14H); 1.68-1.19 (m, 10H); 0.79 (t, 3H). Mass: 459 [M+1]. N-propyl-N-[6-[2-(4-hvdroχyphenyl)ethylaminoIhexyl1- .4-dιhvdro-7.8- dihydroxy-3-H (2H)-benzopyranyllamine dihvdrobromide (Compound 10) as a light brown solid (68% yield) "•H-NMR (?O0 MH7, n-O): r (ppm)- 7 03-6.66 (tn, 4H); 6.46 (d, 1H); 6.38 (d, 1H); 4.44-4.13 (m, 2H) ; 3.83-3.75 (m, 1H); 3.18-2.70 (tn, 12H); 1.63-1.08 (tn, 10H); 0.76 (t, 3H). Mass: 443 [M+1 ]. N-propyl-N-[6-[2-(2.3-dihvdro-2-oxo-6-benzothiazolyl)ethylaminoIhe¬ xyl1-3.4-dihvdro-7.8-dihvdroxy-3-[1 (2H)-benzopyranyllamine dihydro- brotnide (Compound 11) as a light brown solid (66% yield). "• H-NMR (200 MHz, D a 0): δ (ppm): 7.23-6.93 (m, 3H); 6.43 (d, 1H); 6.36 (d, 1H); 4.39-4.13 (m, 2H); 3.80-3.72 (m, 1H); 3.17-2.80 (m, !2H); 1.63-1.12 (tn, 10H); 0.81-0.74 (tn, 3H). Mass: 500 [M+1 ]. (R)-N-propyl-N-[6-[2-(2.3-dihvdro-2-oxo-6-benzothιazolyl)ethylami¬ noIhexyl1-3.4-dihvdro-8-hydroxy-3-[1 (2H)-benzopyranyllamine dihvdrobromide (Compound 12) as a light brown solid (45% yield). 'H-NMR (200 MHz, DMS0-d*): δ (ppm): 11.90 (bs, 1H); 9.60 (bs, 1H); 9.14 (bs, 1H); 8.54 (bs, 2H); 7.48 (d, 1H); 7.17 (dd, 1H); 7.08 (d, 1H); 6.77-6.55 (tn, 3H); 4.52-4.24 (m, 2H) ; 3.96-3.86 (m, 1H); 3.25-2.85 (tn, 12H); 1.78-1.32 (m, 10H); 0.91 (t, 3H). Mass: 484 [M+1]. Example 14 Preparation of N-PΓOPVI-N- Γ6-[2-(4-methoxyphenyl)ethylaminoIhexyl1- 3.4-dihvdro-7.8-dihydroxy-3- 11 (2H)-benzopyranyllamine dihydrochlor¬ ide (Compound 13) ~ Ethanthiol (3.3 ml; 45 moles) was added to a suspension of sodium hydride (1.12 g; 80% in oil; 37.5 mmoles) in anhydrous N, N-ditnethylformamide (10 ml), cooled at 0°C and under nitrogen, and the mixture was kept under stirring at room temperature for 15 minutes. 0 After addition of N-propyl-N-[2-(4-methoxyphenyl)ethylamino]hexyl]- 3,^-dihydro-7,8-dimethoxy-3-[1 (2H)-benzopyrany1lamine riihyd , nrhl< ~ >- ride (1.7 g; 3 mmoles), prepared as described in example 12, the reaction mixture was kept under reflux for 5 hours. After evaporation of the solvent to dryness, the residue was col- lected with water and extracted with ethyl ether. The aqueous phase was acidified and extracted again with ethyl acetate. The organic phase was dried on anhydrous sodium sulphate and con¬ centrated under vacuum. The resultant crude residue (0.6 g) was purified by flash chromato¬ graphy (eluent CH a Cl a :CH 3 OH:HC00H:H a 0=80:20:2:2) obtaining Compound 13 (0.3 g; 19% yield) as a nut-brown solid. m.p. 103°C (dec.) "• H-NMR (200 MHz, D a 0): δ (ppm): 7.07-6.71 (m, 4H); 6.59 (s, 2H); 4.48-4.18 (m, 2H); 3.88-3.79 (tn, 1H); 3.68 (s, 3H); 3.25-2.74 (m, 12H); 1.65-1.14 (m, 10H); 0.80 (t, 3H). Mass: 457 [M+1 ] . Example 15 Dopaminergic functional studies on isolated tissues Evaluation of Di-like activity in the Rabbit Splenic Arterv (RSA) _______ Arterial rings were prepared according to Semeraro et al., Naunyn. Schmied. Arch. Pharmacol., 1990, 342. 539. 5 The arterial preparations were contracted with U46619 (9,11-di- deoxy-1 lα,9α-epoxy-methanoprostaglandin Fa < _) at the submaximal con¬ centration of 0.1 μM. The tested compounds were cumulatively administered. Dopamine and 7,8-dihydroxy-3,4-dihydro-3-(di-n.propylamino)-l (2H)- IQ enzopyran (Ref. 1), described in Eur. J. Med. Chem. (1991), 26, 497-504, were used as reference substances. The agonist activity expressed as pD2 is reported in table 1. Evaluation of D.-like activity in the Rabbit Ear Artery (REA) test Arterial rings were prepared according to the method described by 15 Steinsland et al., Science, 1978, 443. 199, modified as follows. Male New Zealand rabbits weighing 2.5-3 Kg were sacrificed by intravenous injection of an excess of pentobarbital sodium and exsanguinated. The two ears were taken away and the central ear artery was dissected into 3 mm long rings. 20 These preparations were set up in a 25 ml organ bath containing Krebs solution (mM/1): NaCl 118, KCl 4.7, CaCl 2 2.5, MgSO * 1.2, NaHC0 3 25, IHaPO * 1.2, glucose 11.1, equilibrated with 95% 0 a -5% C0 a and maintained at 35±1°C. Krebs solution was medicated with EDTA (10 μM) to prevent cathecol- - amine oxidation desipra ine (O.lμM) and corticosterone (30 μM), to block neuronal and extraneuronal cathecolamine uptake. The preparations were electrically stimulated (10 Hz, 1 msec, 40-80 tnA, 500 msec duration) at 5 minutes intervals. The tested compounds were cumulatively administered. Dopamine and Ref. 1 were used as reference substances. 30 The agonist activity expressed as pD2 is reported in table 1. Table 1 Di-like and D 2 -like activity of compounds 3, 6, 8-11, dopamine and Ref. 1 determined by RSA and REA tests respectively, expressed as pD2. The above data show that the compounds of formula I, object of the present invention, have a significant dopaminergic activity. This activity is higher than that of the reference substances.

Claims: clai s 1 ) A compound of formula CH 2 —CH 2 —_H 3 R" wherein . is an integer number selected among 4, 5, 6, 7 and 8; 0 Z is 0, S, SO or S0 a ; R, R' and R" are hydrogen atoms or OH groups, provided that at least one among R, R' and R" is a hydrogen atom but R, R 1 and R" are not all contemporaneously hydrogen atoms and R' and R" are not both contemporaneously OH groups; 5 or one of R' and R" is a NHCHO, NHCH 3 , NHS0 a CH 3 , CH a 0H or CH 3 group and the other is hydrogen; n_ is an integer number selected among 0, 1, 2, 3 and 4; p_ is an integer number selected between 0 and 1 ; Ri is a hydrogen atom or a Ci-C * alkyl group; - Ar is a phenyl of formula 5 wherein R 2 , R 3 and R * , the same or different, are hydrogen, OH, halogen, Ci-C * alkyl, Ci-C * alkoxy, nitro, Ci-C * alkylthio, NH 2 , ono- 0 or di-d-C * alkylatninc SH, Ci-C * alkylsulphonyl, Ci-C * alkoxycarbonyl, NHCHO, Ci-C * alkylcarbonylamino, NHC0NH 2 , Ci-C * alkyIsulphonylamino, Ci-C * alkylaminosulphonyl, S0 2 NH 2 , 5 NHS0 2 NH 2 , COOH, S0 3 H, C0NH 2 , CH a 0H or phenyl; or R a and R 3 , in ortho position one with respect to the other, together form an optionally unsaturated chain made by 3 or 4 groups selected among CR T "R ΣV , CO, S, 0 and NR V , wherein R" τ is a hydrogen atom or a Ci-C * alkyl group, R IV is a hydrogen 10 atom, a Ci-C * alkyl group or an amino group and R v is a hvdrogen atom or a Ci-C * alk v l ■ σ oϋ r '* or P."' to σ ether with a vicinal R" 1 or R v constitutes a single bond or R v together with a vicinal R" τ or R v constitutes a single bond; or a 5 or 6 membered aromatic heterocycle containing one or more 15 heteroatoms selected among oxygen, nitrogen and sulphur, option¬ ally substituted by from one to three substituents selected among halogen, OH, Ci-C * alkyl or alkoxy; Y is S, 0, N(R s )C0, C0(R β )N or N(R B ); X is N(R«), 0, S, SO, SOa, CO or a single bond; 20 R= is a hydrogen atom or a Ci-C * alkyl group; R_ is a hydrogen atom; or R B and R β together form a -CH a - or -CHa-CH 2 - chain; or R 2 , when in ortho position with respect to X, constitutes a single - c bond or form a -CH 2 - or -CH 2 -CH a - chain with R B ; or _ when X=0 Ra, when in ortho position with respect to X, forms a -CH a -0- chain with R n ; provided that when p=1 , X is an N(R*); the asterisk marks an asymmetric carbon atom; 30 and pharmaceutically acceptable salts thereof. 2) A compound according to claim 1 wherein R' is a hydrogen atom, R and R" are OH groups. 53) A compound according to claim 1 wherein R' is a hydrogen atom, R and R" are OH groups and . is 6. 4) A process for the preparation of a compound according to claim 1 comprising the reduction of a compound of formula CH a ~CH a ~CH 3 0 R- wherein π_, £_ Z, Y, X, and Ar have the meanings already reported in claim 1 ; 5 R-7, Rβ and R* are hydrogen atoms or OA groups wherein A is a hydrogen atom or a protective group selected among methyl, benz¬ yl, benzoyl and 4-methoxybenzoyl, provided that at least one among R-7, Rβ and R v is a hydrogen atom but R-7, R β and R v are not all contemporaneously hydrogen atoms and R β and R v are not both 0 contemporaneously OA groups; or one of R θ and R, is an NHCHO, NHCH 3 , NHS0 2 CH 3 , CH 2 0H or CH 3 group and the other is hydrogen; U is a CH 2 or CO group; Q is a group of formula 5 C0-(CH 2 ) n -ι-CHRι wherein Ri has the meanings already reported in claim 1 , when n. is 1 , 2, 3 or 4; or Q is a CHR, or CO group, wherein R, has the meanings already reported in claim 1, when n_ is 0. 0 5) A pharmaceutical composition containing a therapeutically effec¬ tive amount of a compound according to claim 1 in admixture with a suitable carrier. 56) A pharmaceutical composition according to claim 5 for the treat¬ ment of cardiovascular diseases. 0

Inventors: CAVALLERI PAOLO, FRAIRE CRISTINA, GRANCINI GIAN CARLO, MONTANARI STEFANIA, NAPOLETANO MAURO, SANTANGELO FRANCESCO, CAVALLERI, PAOLO, FRAIRE, CRISTINA, GRANCINI, GIAN, CARLO, MONTANARI, STEFANIA, NAPOLETANO, MAURO, SANTANGELO, FRANCESCO
Applicants: CAVALLERI PAOLO, FRAIRE CRISTINA, GRANCINI GIAN CARLO, MONTANARI STEFANIA, NAPOLETANO MAURO, SANTANGELO FRANCESCO, ZAMBON SPA, CAVALLERI, PAOLO, FRAIRE, CRISTINA, GRANCINI, GIAN, CARLO, MONTANARI, STEFANIA, NAPOLETANO, MAURO, SANTANGELO, FRANCESCO, ZAMBON GROUP S.P.A.
Application year: 1995
Main classification: C07D417
IPC codes: A61K31, A61P9, A61P13, A61P15, A61P43, C07C215, C07D311, C07D335, C07D417