HUMAN MUTATOR GENE hMSH2 AND HEREDITARY NON POLYPOSIS COLORECTAL CANCER

Abstract: The human MSH2 gene, responsible for hereditary non-polyposis colorectal cancer, was identified by virtue of its homology to the MutS class of genes, which are involved in DNA mismatch repair. The sequence of cDNA clones of the human gene are provided, and the sequence of the gene can be used to demonstrate the existence of germ line mutations in hereditary non-polyposis colorectal cancer (HNPCC) kindreds, as well as in replication error<+> (RER<+>) tumor cells.

Description: HUMAN MUTATOR GENE hMSH2 AND HEREDITARY NON POLYPOSIS COLORECTAL CANCER This invention was made using U.S. government grants from the NIH CA47527, CA09320, GM26449, CA09243, CA41183, CA42705 CA57435, and CA35494, as well as grants from the Department of Energy DOE/ERN/F139 and DE-FG 09291ER-61139. Therefore the U.S. government retains certain rights to the invention. This application is a continuation-in-part of Serial No. 08/056,546, filed May 5, 1993. TECHNICAL FIELD OF THE INVENTION The invention relates to a gene which predisposes individuals to colorectal and other cancers. In addition, it also relates to biochemical tests which can be used to identify drugs for treatment of affected individuals. BACKGROUND OF THE INVENTION HNPCC (Lynch syndrome) is one of the most common cancer predisposition syndromes, affecting as many as 1 in 200 individuals in the western world (Lynch et al., 1993). Affected individuals develop tumors of the colon, endometrium, ovary and other organs, often before 50 years of age. Although the familial nature of this syndrome was discovered nearly a century ago (Warthin et al., 1913), the role of heredity in its causation remained difficult to define (Lynch et al., 1966). Recently, however, linkage analysis in two large kindreds demonstrated association with polymorphic markers on chromosome 2 (Peltomaki et al., 1993a). Studies in other families suggested that neoplasia in a major fraction of HNPCC kindreds is linked to this same chromosome 2p locus (Aaltonen et al., 1993). HNPCC is defined clinically by the occurrence of early-onset colon and other specific cancers in first degree relatives spanning at least two generations (Lynch et al., 1993). The predisposition is inherited in an autosomal dominant fashion. It was initially expected that the gene(s) responsible for HNPCC was a tumor suppressor gene, as other previously characterized cancer predisposition syndromes with this mode of inheritance are caused by suppressor gene mutations (reviewed in Knudson, 1993). But the analysis of tumors from HNPCC patients suggested a different mechanism. Most loci encoding tumor suppressor genes undergo somatic losses during tumorigenesis (Stanbridge, 1990). In contrast, both alleles of chromosome 2p loci were found to be retained in HNPCC tumors (Aaltonen et al., 1993). During this search for chromosome 2 losses, however, it was noted that HNPCC tumors exhibited somatic alterations of numerous microsatellite sequences. Widespread, subtle alterations of the cancer cell genome were first detected in a subset of sporadic colorectal tumors using the arbitrarily-primed polymerase chain reaction (Peinado et al., 1992). These alterations were subsequently found to represent deletions of up to 4 nucleotides in genomic polyA tracts (Ionov et al. , 1993). Other studies showed that a similar, distinctive subgroup of sporadic tumors had insertions or deletions in a variety of simple repeated sequences, particularly microsatellite sequences consisting of dinucleotide or trinucleotide repeats (Ionov et al., 1993; Thibodeau et al., 1993; Aaltonen et al., 1993). Interestingly, these sporadic tumors had certain features in common with those developing in HNPCC kindreds, such as a tendency to be located on the right side of the colon and to be near-diploid. These and other data suggested that HNPCC and a subset of sporadic tumors were associated with a heritable defect causing replication errors (RER) of microsatellites (Ionov et al., 1993; Aaltonen et al., 1993). The mechanism underlying the postulated defect could not be determined from the study of tumor DNA, but studies in simpler organisms provided an intriguing possibility (Levinson and Gutman, 1987; Strand et al., 1993). This work showed that bacteria and yeast containing defective mismatch repair genes manifest instability of dinucleotide repeats. The disruption of genes primarily involved in DNA replication or recombination had no apparent effect on the fidelity of microsatellite replication (reviewed in Kunkel, 1993). These pivotal studies suggested that defective mismatch repair might be responsible for the microsatellite alterations in the tumors from HNPCC patients (Strand et al. , 1993). Thus there is a need in the art to identify the actual gene and protein responsible for hereditary non-polyposis colorectal cancer and the replication error phenotype found in both hereditary and sporadic tumors. Identification of the gene and protein would allow more widespread diagnostic screening for hereditary non- polyposis colorectal cancer than is currently possible. Identification of the involved gene and protein would also enable the rational screening of compounds for use in drug therapy of hereditary non-polyposis colorectal cancer, and would enable gene therapy for affected individuals. SUMMARY OF THE INVENTION It is an object of the invention to provide a DNA molecule which when mutated is the genetic determinant for hereditary non-polyposis colorectal cancer. It is another object of the invention to provide DNA molecules which contain specific mutations which cause hereditary non-polyposis colorectal cancer. It is yet another object of the invention to provide methods of treating persons who are predisposed to hereditary non-polyposis colorectal cancer. It is still another object of the invention to provide methods for determining a predisposition to cancer. It is a further object of the invention to provide methods for screening test compounds to identify therapeutic agents for treating persons predisposed to hereditary non-polyposis colorectal cancer. It is still another object of the invention to provide a protein which is important for human DNA mismatch repair. It is yet another object of the invention to provide a transgenic animal for studying potential therapies for hereditary non-polyposis colorectal cancer. These and other objects of the invention are provided by one or more of the embodiments described below. In one embodiment of the invention an isolated and purified DNA molecule is provided. The molecule has a sequence of at least about 20 nucleotides of HMSH2, as shown in SEQ ID NO:l. In another embodiment of the invention an isolated and purified DNA molecule is provided. The DNA molecule has a sequence of at least about 20 nucleotides of an hMSH2 allele found in a tumor wherein said DNA molecule contains a mutation relative to HMSH2 shown in SEQ ID NO:l. In yet another embodiment of the invention a method of treating a person predisposed to hereditary non-polyposis colorectal cancer is provided. The method prevents accumulation of somatic mutations. The method involves administering a DNA molecule which has a sequence of at least about 20 nucleotides of hMSH2, as shown in SEQ ID NO:l, to a person having a mutation in an hMSH2 allele which predisposes the person to hereditary non-polyposis colorectal cancer, wherein said DNA molecule is sufficient to remedy the mutation in an hMSH2 allele of the person. In another embodiment of the invention a method is provided for determining a predisposition to cancer. The method involves testing a body sample of a human to ascertain the presence of a mutation in hMSH2 which affects hMSH2 expression or HMSH2 protein function, the presence of such a mutation indicating a predisposition to cancer. In still another embodiment of the invention a method is provided for screening to identify therapeutic agents which can prevent or ameliorate tumors. The screening method involves contacting a test compound with a purified hMSH2 protein or a cell; determining the ability of the hMSH2 protein or the cell to perform DNA mismatch repair, a test compound which increases the ability of said hMSH2 protein or said cell to perform DNA mismatch repair being a potential therapeutic agent. In another embodiment of the invention an isolated and purified protein is provided. The protein has the sequence shown in SEQ ID NO:2. In still another embodiment of the invention a transgenic animal is provided. The transgenic (nonhuman) animal maintains an hMSH2 allele in its germline. The HMSH2 allele is one which is found in humans having hereditary non-polyposis colorectal cancer or in RER + tumors. Also provided are animals which have no wild-type MSH2 alleles, due to mutations introduced. Thus the present invention provides the art with the sequence of the gene responsible for hereditary non-polyposis colorectal cancer and information regarding the mechanism by which it causes tumors. This enables the art to practice a variety of techniques to identify persons at risk of developing a variety of cancers and to treat them to prevent such cancers from actually developing. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 summarizes the markers retained in somatic cell hybrids used in locating the hMSH2 gene. PCR was used to determine whether each of the listed markers was present (black box) or absent (white box)in the indicated hybrid. The laboratory name of each hybrid and the formal name (in parentheses) is listed. The hybrid panel was also validated with ten additional polymorphic markers outside of the 136-177 region. M: hybrid derived from microcell-mediated chromosome 2 transfer; T: derived from t(X;2) translocation; X: derived from X-irradiated chromosome 2 donor. MO: mouse-human hybrid; HA: hamster-human hybrid; RA: rat-human hybrid; TEL: telomere; CEN: centromere. Figure 2 shows a FISH analysis which was used to determine the proximity and ordering of DNA sequences within chromosome band 2pl6. Panels 2A and 2B show FISH mapping of the 123 marker. Panel 2A shows G-banded metaphase chromosome 2. Panel 2B shows identical chromosome as in Panel 2A following FISH with a biotin-labeled PI clone for the 123 marker. Results localize the 123 marker to chromosome band 2pl6.3. Panels 2C and 2D show co-hybridization documenting the coincident localization of a microdissection (Micro-FISH) probe from chromosome 2pl6 and the 123 marker. Panel 2C shows DAPI stained metaphase chromosome 2. Panel 2D shows simultaneous hybridization of the biotin-labeled 123 probe (appearing as an intensely staining smaller circle) and the Spectrum-Orange labeled 2pl6 Micro-FISH probe (appearing as a diffusely staining larger circle). Panel 2E shows a representative example of an interphase nucleus simultaneously hybridized with PI clones for CA5, hMSH2 and ze3. The results were used to directly measure the distances between markers in order to establish the order and relative distance between markers (after Trask et al., 1989). Inset: The image processing program NIH Image was used to provide an average gray value displayed as a surface plot to support the length measurements and to graphically illustrate the relative order information. The surface plot presented defines the specified interphase chromosome and the relative order CA- SH2-ze3. Figure 3 shows linkage analysis of ΗNPCC pedigrees. All affected individuals in which meiotic recombination occurred between markers 119 and 136 are included. A black box indicates that the individual did not contain the allele associated with disease in his/her family or that the individual inherited an allele not associated with disease from his/her affected parent. A white box indicates that the individual had an allele which was the same size as the disease-associated allele. A hatched circle indicates that the marker was not studied. All individuals had colon or endometrial cancer at less than 55 years of age, or had progeny with such disease but did not indicate that the patient necessarily had disease-associated alleles because phase could usually not be determined. Figure 4 shows HMSH2 gene localization. Southern blots containing EcoRI (figures 4A and 4C) and PstI (figures 4B and 4D) digested DNA from the indicated somatic cell hybrids (figures 4A and 4B) or YAC clones (figures 4C and 4D) were hybridized with a radiolabelled insert from cDNA clone pNP-23. Southern blotting and hybridization were performed as described (Vogelstein et al., 1987). Autoradiographs are shown. The 5.0 kb PstI fragment in hybrids Zll and Z12 is derived from hamster DNA. Figure 5 shows the cDNA sequence of hMSH2. An open reading frame (ORF) begins at nucleotide 1 and ends at nt 2802. The predicted amino acid sequence is shown. The sequence downstream of nt 2879 was not determined. Figure 6 shows homology between yeast and human AfSH2 genes. The predicted amino acid sequences of yeast (y) MSH2 (Reenan and Kolodner, 1992) and human MSH2 genes are compared within the region of highest homology. Blocks of similar amino acids are shaded. Figure 7 shows germline and somatic mutations of hMSH2. Autoradiographs of polyacrylamide gels containing the sequencing reactions derived from PCR products are shown. The 1.4 kb PCR products containing a conserved region of hMSH2 were generated from genomic DNA samples as described in the Examples. Antisense primers were used in the sequencing reactions. The ddA mixes from each sequencing reaction were loaded in adjacent lanes to facilitate comparison, as were those for C, G, and T. The DNA samples were derived from the tumor (lane 1) and normal colon (lane 2) of patient CxlO, an RER- colon tumor cell line (lane 3), and lymphocytes of patients J-42 (lane 4) and J-143 (lane 5). Figure 7A: A transition (C to T at codon 622) in lymphocyte DNA can be observed in ΗNPCC patients J-42 and J-143. Figure 7B: A transition (C to T at nt codon 639) in tumor (lane 1) and normal colonic mucosa (lane 2) of patient CxlO. Figure 7C: A substitution of a TG dinucleotide for an A at codon 663 can be observed in DNA of the tumor of patient CxlO, (lane 1), but not in DNA from her normal colon (lane 2). Arrows mark the substitutions in panel A and B and the TG dinucleotide insertion site in panel C. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The disclosure of Serial No. 08/056,546, filed May 5, 1993, is expressly incorporated herein. It is a discovery of the present invention that the gene responsible for hereditary non-polyposis colorectal cancer is hMSH2, a human analog of bacterial MutS. The cDNA sequence of hMSH2 is shown in SEQ ID NO:l. This gene encodes a DNA mismatch repair enzyme. Mutation of the gene causes cells to accumulate mutations. For example, the observed replication error phenotype (RER + ) found in both sporadic and hereditary non-polyposis colorectal cancer consists of variations (insertions and deletions) in microsatellite DNA. In yeast and bacteria defective AfwrS-related genes cause other types of mutations as well. Useful DNA molecules according to the invention are those which will specifically hybridize to HMSH2 sequences. Typically these are at least about 20 nucleotides in length and have the nucleotide sequence as shown in SEQ ID NO: 1. Such molecules can be labeled, according to any technique known in the art, such as with radiolabels, fluorescent labels, enzymatic labels, sequence tags, etc. According to another aspect of the invention, the DNA molecules contain a mutation which has been found in tumors of HNPCC patients or in sporadic RER + tumors. Such molecules can be used as allele-specific oligonucleotide probes to track a particular mutation through a family. According to some aspects of the invention, it is desirable that the DNA encode all or a part of the MSH2 protein as shown in SEQ ID NO:2. To obtain expression of the protein the DNA sequence can be operably linked to appropriate control sequences, such as promotor, Kozak consensus, and terminator sequences. A person who is predisposed to develop cancers due to inheritance of a mutant HMSH2 allele can be treated by administration of a DNA molecule which contains all or a part of the normal HMSH2 gene sequence as shown in SEQ ID NO:l. A portion of the gene sequence will be useful when it spans the location of the mutation which is present in the mutant allele, so that a double recombination event between the mutant allele and the normal portion "corrects" the defect present in the person. A portion of the gene can also be usefully administered when it encodes enough of the protein to express a functional DNA mismatch repair enzyme. Such a portion need not necessarily recombine with the mutant allele, but can be maintained at a separate locus in the genome or on an independently replicating vector. Means for administering DNA to humans are known in the art, and any can be used as is convenient. A variety of vectors are also known for this purpose. According to some techniques vectors are not required. Such techniques are well known to those of skill in the art. Also contemplated as part of the present invention is the use of a combined anti-neoplastic therapy regimen. Such a combined regimen is useful for patients having an RER + tumor, whether sporadic or associated with HNPCC. The regimen combines any standard anti-neoplastic therapy to which a patient can become resistant and HMSH2 gene therapy, as described above. By remedying the defect present in RER + cells, i.e. , an hMSH2 mutation, the likelihood of the tumor developing a resistance mutation is greatly diminished. By delaying or preventing the onset of resistance, the life of cancer patients can be prolonged. In addition, such prevention of resistance allows a greater degree of tumor destruction by the therapeutic agent. Examples of anti-neoplastic therapies which can be combined with hMSH2 gene therapy are hormones, radiation, cytotoxic drugs, cytotoxins, and antibodies. Body samples can be tested to determine whether the hMSH2 gene is normal or mutant. Mutations are those deviations from the sequence shown in SEQ ID NO:l which are associated with disease and which cause a change in hMSH2 protein function or expression. Such mutations include nonconservative amino acid substitutions, deletions, premature terminations and frameshifts. See Table I. Suitable body samples for testing include those comprising DNA, RNA, or protein, obtained from biopsies, blood, prenatal, or embryonic tissues, for example. Provided with the information that the defect causing HNPCC and sporadic RER + tumors is in a DNA mismatch repair enzyme, one can perform assays on test compounds and compositions to determine if they will remedy the defect. Such therapeutic compounds could bind to missense hMSH mutant proteins to restore the proteins to the normal, active conformation. Alternatively such therapeutic compounds could stimulate the expression of alternate pathways for mismatch repair. Screening for such therapeutic compounds could be performed by contacting test compounds with cells, either normal or those with an hMSH2 mutation found in a tumor. The ability of the cells which were contacted with the test compounds is compared with the ability of the same cells which were not contacted for mismatch repair activity. Such activity can be tested as is known in the art. See, for example, Levinson and Gutman, 1987, and Strand et al., 1993. Observation of changes in microsatellite DNA in cells is one way of assessing mismatch repair activity. Another approach is to assay DNA mismatch repair in vitro in nuclear extracts. See Holmes, 1990; Thomas, 1991; and Fang, 1993. Table 1 CO CO _33 r Provided with the cDNA sequence and the amino acid of hMSH2 protein, one of ordinary skill in the art can readily produce hMSH2 protein, isolated and purified from other human proteins. For example, recombinant cells or organisms can be used to produce the protein in bacteria, yeast, or other convenient cell system. The isolated and purified protein can be used in screening for new therapeutic agents, for example, in in vitro assays of DNA mismatch repair. The protein can also be used to raise antibodies against hMSH2. Therapeutic administration of the protein is also contemplated. Transgenic animals are also contemplated by the present invention. These animals would have inserted in their germline hMSH2 alleles which are associated with HNPCC or sporadic tumors. Such animals could provide model systems for testing drugs and other therapeutic agents to prevent or retard the development of tumors. Also contemplated are genetically engineered animals which contain one or more mutations in their own MSH2 genes. The mutations will be engineered to correspond to mutations found in hMSH2 alleles which are found in HNPCC- affected individuals or in other human RER + tumors. Animals with both native MSH2 alleles inactivated and containing a human wild-type or mutant MSH2 allele are particularly desirable. Examples Example 1 Somatic Cell Hybrids A panel of human-hamster, human-mouse, and human-rat hybrid cell lines was developed to facilitate HNPCC mapping. Hybrids containing only portions of chromosome 2 were obtained by microcell-mediated chromosome transfer or by standard cell fusions following X-irradiation of the chromosome 2 donor. Additionally, two hybrids were used which contained a (X;2)(q28;p21) translocation derived from human fibroblasts. In previous studies, the HNPCC locus was mapped to the 25 cM region surrounding marker 123 and bordered by markers 119 and 136 (Peltomaki et al., 1993a). Thirty-eight hybrids were screened with these three chromosome 2p markers. Eight of the hybrids proved useful for mapping the relevant portion of chromosome 2p. For example, hybrids LI and L2 contained the distal half of the region, including marker 123, while hybrid y3 contained the half proximal to marker 123 (Figure 1). Methods Methods for the derivation of microcell-mediated chromosome 2 hybrids have been described previously (Chen et al., 1992; Spurr et al., 1993). Some hybrids were generated following fusion of X-irradiated donor cells containing human chromosome 2 to CHO cells (Chen et al., 1994). Mouse hybrids were derived by fusing HPRT deficient L cells (A9) with human fibroblasts (GM7503) containing a t(X;2)(q28,p21) translocation and selecting in media containing HAT. Example 2 Polymorphic Markers To map more finely the HNPCC locus, additional polymorphic markers were obtained in three ways. First, a genomic clone containing 85 kb surrounding the 123 marker was used for fluorescence in situ hybridization (FISH) to localize it to chromosomal band 2pl6.3 (Figure 2A,B). The 2pl6 band region was then microdissected, and the sequences within this band were amplified using the polymerase chain reaction and subcloned into plasmid vectors (see Experimental Procedures). The accuracy of the microdissection was confirmed using dual-color FISH by simultaneously hybridizing to microdissected material and a genomic clone containing marker 123 (Figure 2C,D). The subclones were screened by hybridization to a (CA) 15 probe, and hybridizing clones identified and sequenced. These sequences were then used to design oligonucleotide primers for PCR analysis of genomic DNA. Nineteen (CA) n repeat markers were identified in this way. Of these, four were highly polymorphic and mapped to the region between markers 119 and 136, as assessed by the somatic cell hybrid panel exhibited in Figure 1. Second, eight additional (CA) n markers, cloned randomly from human genomic DNA using a poly (CA) probe, were found to lie between markers 119 and 136 by linkage analysis in CEPH pedigrees. Five of these were particularly informative and were used in our subsequent studies. Finally, one additional marker was identified by screening subclones of a genomic PI clone containing marker 123 with a (CA) 15 probe. Through these analyses, thirteen new polymorphic markers were identified in the 25 cM interval between markers 119 and 136, resulting in an average marker spacing of ~2 cM (Table π). These markers were mapped with respect to one another by linkage in CEPH and HNPCC pedigrees as well as by analysis of somatic cell hybrids. These two mapping techniques provided consistent and complementary information. For example, the relative positions of CA16 and CA18 could not be distinguished through linkage analysis but could be determined with the somatic cell hybrids LI , L2, and Y3. Conversely, the relative position of the ze3 and yh5 markers could not be determined through somatic cell hybrid mapping, but could be discerned by linkage analysis. TABLE II HETEROZYGOSITY 0.84 0.77 0.76 0.61 0.77 0.78 0.76 0.75 0.71 0.69 m 0.80 0.76 5D 0.79 m 0.73 . ren 0.73 0.76 387.388, 389 Methods All markers were obtained by screening human genomic libraries with radiolabelled (CA) n probes (Weber and May, 1989). The "T" markers (see Table 1) were generated from a library made from total human genomic DNA, as described in Weissenbach et al., 1992. The "M" markers were made from libraries generated from microdissected chromosome 2pl6, as described below. The CA2 marker was generated from a library made from PI clone 210 digested to completion with Sau3 and cloned into the Xhol site of lambda YES (EUedge et al., 1991). The sequences of the clones obtained from these libraries were determined, and primers surrounding the CA repeats chosen. Only primers giving robust amplification and high heterozygosity were used for detailed analysis of HNPCC kindreds. All markers used in this study were shown to be derived from chromosome 2p by both linkage analysis in the CEPH pedigrees and evaluation in the somatic cell hybrid panel shown in Figure 1. The sequences of the primers and other details specific for each marker have been deposited with the Genome Data Base. Linkage analyses to obtain the map of the marker loci in CEPH families 1331, 1332, 1347, 1362, 1413, 1416, 884, and 102 (Weissenbach et al., 1992) were performed using the CLINK program of the LINKAGE program package (Lathrop et al., 1984) with the no sex difference option and 11-point computations. The odds for the best locus order supported by the data were evaluated against pairwise inversions of the loci. Example 3 Genomic Clones Many of the polymorphic markers shown in Table 1 were used to derive genomic clones containing 2pl6 sequences. Genomic clones were obtained by PCR screening of human PI and YAC libraries with these polymorphic markers, with ten additional sequence tagged sites (STS) derived from chromosome 2pl6 microdissection, or with YAC junctions. Twenty-three PI clones, each containing 85-95 kb, were obtained, as well as 35 YAC clones, containing 300 to 1800 kb. The YAC clones in some cases confirmed the linkage and somatic cell hybrid maps. For example, markers ze3 and yh5 were both found in YAC's 4F4 and 1E1 while CA16 and CA18 were both found in YAC 8E5, documenting their proximity. The highest density of genomic clones (28 YAC and 17 PI clones) was obtained between markers yb9 and yh5 (Table 1), which became the region most likely to contain the HNPCC gene during the course of these studies. The region between yh5 and yb9 was predicted to contain ~ 9 Mb (assuming 1 Mb/per cM). Based on the sizes of the YAC clones, and taking into account their chimerism, we estimated that they contained over 70% of the sequences between yh5 and yb9. Methods The markers described in Table 1 were used to screen YAC or PI libraries by PCR. The CEPH A library was obtained from Research Genetics, Inc. and consisted of 21,000 YAC clones, arrayed in a format allowing facile screening and unambiguous identification of positive clones. The sizes of ten of the YAC clones containing markers were determined by transverse alternating pulse-field gel electrophoresis using a GeneLine π apparatus from Beckman and found to average 0.7 Mb (range 0.2-1.8 Mb). In some cases, inverse PCR was used to determine the YAC junctions (Joslyn et al., 1991), and the derived sequence used for "chromosome walking" with the YAC or PI libraries. The junctions were also used to design primers to test whether the ends of the YAC clones could be localized to chromosome 2pl6 (and therefore presumably be non-chimeric). Three of four YAC clones which were tested in this way had both ends within the expected region of chromosome 2, as judged by analysis with the somatic cell hybrid panel. The human genomic PI library was also screened by PCR (Genome Systems, Inc.). PI clones M1015 and M1016, containing the hMSH2 gene, were used to determine intron-exon borders using sequencing primers from the exons and SequiTherm™ polymerase (Epicentre Technologies). Example 4 Analysis of HNPCC Families The markers described in Table 1 were then used to analyze six large HNPCC kindreds previously linked to chromosome 2p (Peltomaki et al., 1993a). Two hundred thirteen individuals, including 56 members affected with colorectal or endometrial cancer, were examined. Four of the kindreds were from the United States, one from Newfoundland and one from New Zealand. To increase the number of affected individuals that could be examined, we obtained formalin-fixed, paraffin-embedded sections of normal tissues from deceased individuals and purified DNA from them (Goelz et al., 1985a). A single allele of each of the thirteen markers was found to segregate with disease in each of the six families (i.e., the allele was found in over 50% of affected individuals). No allele of any marker was shared among the affected members of more than three kindreds. Fourteen of the affected members contained only a subset of the expected alleles and therefore had undergone recombination between markers 119 and 136. Eleven of these individuals appeared to have simple, single recombination events. The most informative of these was in individual 148 from the J kindred and individual 44 from kindred 621 (Figure 3). Individual 621-44 apparently retained the disease linked allele at markers distal to CA5, while demonstrating multiple recombinants at more proximal loci, thus placing the CA5 marker at the proximal border of the HNPCC locus. Individual J-148 apparently retained the disease- linked allele at all markers proximal to yh5, while exhibiting recombinants at yh5 and 119, thus placing the distal border at yh5. Assuming that the same gene was involved in both the J and 621 kindreds, the HNPCC gene was predicted to reside between markers CA5 and yh5, an area spanning approximately 2 cM (Table 1). The DNA of three affected individuals (C-202, 4-156, 4-92) appeared to have undergone two recombinations in the area. There was probably one recombinant per generation, and this could be demonstrated in C-202 by analysis of DNA from his parents; in the other cases, parental DNA was not available. All three individuals retained disease-linked alleles at CA5 and ze3 but not at more proximal and distal loci (Figure 3). Combined with the data from the patients with single recombinations, the double recombinants suggested that the HNPCC gene resided between CA5 and ze3, a distance spanning less than 2 cM. To determine the physical distances separating CA5, ze3, and yh5, metaphase and interphase FISH analysis was carried out. Dual-color FISH with PI clones containing these markers was performed with PI clones 820 and 838 (containing the markers CA5 and yh5, respectively) labeled with biotin and detected with fluorescein-labelled avidin, and clone 836 (containing marker ze3) labelled with Spectrum-Orange (Meltzer et al., 1992). The hybridization signals of these markers appeared coincident on metaphase chromosomes, confirming that they resided within an interval of < 1.0 Mb. When FISH was performed on interphase nuclei, the relative positions of the three markers could be determined and the distances between them estimated (Trask et al., 1989). The results confirmed that the orientation of the markers was telomere-yh5-ze3-CA5- centromere (data not shown). Direct measurement of the distance between yh5 and ze3 was estimated at <0.3 Mb, consistent with the presence of both of these markers on YAC clones 4E4 and 1E1. Measurements of 48 interphase chromosomes provided an estimate of the distance between ze3 and CA5 at <0.8 Mb, independently confirming the linkage data. Methods G-banded metaphase chromosomes were microdissected with glass microneedles and amplified by PCR as previously described (Guan et al., 1993 Kao and Yu, 1991). For dual-color FISH, the PCR product was fluorochrome labelled (Spectrum-Orange, Imagenetics, Naperville, IL) or biotinylated in a secondary PCR reaction. PI clones were labelled by nick-translation or by degenerate oligonucleotide primers (Guan et al., 1993). FISH was carried out as previously described (Guan et al., 1993) and visualized with a Zeiss Axiophot equipped with a dual-bandpass filter. For analysis of interphase FISH patterns, the distance between hybridization signals was measured in a minimum of 24 nuclei (Trask et al., 1989). Example 5 Candidate Genes On the basis of the mapping results described above, we could determine whether a given gene was a candidate for HNPCC by determining its position relative to the CA5-ze3 domain. The first gene considered was a human homolog of the Drosophila SOS gene (reviewed by Egan and Weinberg, 1993). This gene transmits signals from membrane bound receptors to the ras pathway in diverse eukaryotes. It was considered a candidate because another ras-interacting gene, NF1, causes a cancer predisposition syndrome (Viskochil et al., 1990; Wallace et al., 1990), and SOS has been localized to chromosome 2pl6-21 by in situ hybridization (Webb et al., 1993). Using PCR to amplify SOS sequences from the hybrid panel, however, SOS was found to be distal to the CA5-yh5 domain (present in hybrid Z19 but not Z29). We next examined the interferon-inducible RNA activated protein kinase gene PKR. This gene has been shown to have tumor suppressor ability (reviewed by Lengyel, 1993) and to map close to 2pl6 (Hanash et al., 1993). We could not initially exclude PKR from the HNPCC domain, and therefore determined the sequence of its coding region in two individuals from HNPCC kindred C. Reverse transcriptase was used to generate cDNA from lymphoblastoid derived RNA of these two individuals, and PCR performed with primers specific for PKR. The PKR products were sequenced, and no deviations from the published sequence was identified within the coding region (Meurs et al., 1990). Subsequent studies showed that the PKR gene was distal to the yh5 marker, and thus could be excluded as a basis for HNPCC. We then considered human homologs of the MutL and MutS mismatch repair genes previously shown to produce microsatellite instability in bacteria and yeast when disrupted (Levinson and Gutman, 1987; Strand et al., 1993). A human homolog of the yeast wtL-related gene PAfSi (Kramer et al., 1989) does not appear to reside on chromosome 2p (M. Liskay, personal communication). To identify homologs of MutS, we used degenerate oligonucleotide primers to PCR- amplify cDNA from colon cancer cell lines. The same primers had been previously used to identify the yeast MSH2 gene on the basis of its MutS homology (Reenan and Kolodner, 1992). Under non-stringent conditions of PCR, a fragment of the expected size was obtained and these fragments were cloned into plasmid vectors. Most of the clones contained ribosomal RNA genes, representing abundant transcripts with weak homology to the degenerate primers. A subset of the clones, however, contained sequences similar to that of the yeast A SH2 gene, and one such clone, pNP-23, was evaluated further. The human gene from which this clone is derived is hereafter referred to as hMSH2. The insert from clone pNP-23 was used as a probe in Southern blots of somatic cell hybrid DNA. This insert hybridized to one or two fragments in human genomic DNA digested with PstI or EcoRI, respectively, and these fragments were present in hybrid Z30, containing most of human chromosome 2p. Analysis of other hybrids showed that the fragment was present in hybrids Zll, Z29, LI and L2, but not Z12, Y3 or Z19, thereby localizing the human MSH2 QιMSH2) gene to a region bordered by markers CA18 and 119 (examples in Figure 4). The YAC clones listed in Table 1 were then analyzed, and EcoRI and PstI fragments of the expected size identified in YAC 5A11, derived from screening the YAC library with the CA5 marker (Figure 4). To confirm the Southern blots, we designed non-degenerate primers on the basis of the sequence of pNP-23. Several sets of primers were tested so that genomic DNA could be used as a template for PCR; an intervening intron prevented the original primers from being used effectively with templates other than cDNA. PCR with these primers was perfectly consistent with the Southern blot results. The expected 101 bp fragment was present in hybrids Z4, Zll, Z29, LI, and L2, and in YAC 5A11, but not in other hybrids or YAC clones (not shown). The localization of hMSE.2 sequences to YAC 5A11 demonstrated the proximity of these sequences to marker CA5. To determine the distance and relative orientation of HMSH2 with respect to CA5, we performed interphase FISH analysis. PI clones containing CA5, ze3 and hMSH2 sequences (clones 820, 836, and M1015, respectively) were simultaneously hybridized to interphase nuclei following fluorescein and Spectrum Orange labelling (Meltzer et al., 1992). The results demonstrated that MSH2 resides within the HNPCC locus defined by linkage analysis to lie between CA5 and ze3, and less than 0.3 Mb from marker CA5 (Figure 2E). cDNA libraries generated from human colon cancer cells or from human fetal brain tissues were then screened with the insert of pNP-23 to obtain additional sequences from this gene. Seventy-five cDNA clones were initially identified and partially sequenced. PCR products representing the ends of the cDNA sequence contig were then used as probes to re-screen the cDNA libraries. This cDNA "walk" was repeated again with the new contig ends. Altogether, 147 cDNA clones were identified. The composite sequence derived from these clones is shown in Figure 5. An open reading frame (ORF) began 69 nt downstream of the 5' end of the cDNA contig, and continued for 2802 bp. The methionine initiating this ORF was in a sequence context compatible with efficient translation (Kozak, 1986) and was preceded by in-frame termination codons. RNA from placenta and brain were used in a PCR-based procedure (RACE, Frohman et al., 1988) to independently determine the position of the 5' end of hMSH2 transcripts. This analysis demonstrated that the 5' ends of all detectable transcripts were less than 100 bp upstream of the sequence shown in Figure 5, and were heterogeneous upstream of nt -69. The region of highest homology to the yeast MSH2 gene is shown in Figure 6. This region encompassed the helix-turn-helix domain perhaps responsible for MutS binding to DNA (Reenan and Kolodner, 1992). The yeast and human MSH2 proteins were 77% identical between codons 615 and 788. There were several other blocks of similar amino acids distributed throughout the length of these two proteins (966 and 934 amino acids in yeast and human, respectively). Methods cDNA generated from the RNA of colorectal cancer cells with reverse transcriptase was used as template for PCR with the degenerate primers 5'-CTG GAT CCA C(G/A/T/C) G G(G/A/T/C)C C(G/A/T/C)A A(T/C)A TG-3' and 5'- CTG GAT CC(G/A) TA(G/A) TG(G/A/T/C) GT (G/A/T/C) (G/A)C(G/A) AA-3'. These two primers were used previously to identify the yeast MSH2 gene and were based on sequences conserved among related mammalian and bacterial genes (Reenan and Kolodner, 1992). The optimal PCR conditions for detecting the human MSH2 gene consisted of 35 cycles at 95° for 30 seconds, 41° for 90 seconds, and 70° for 90 seconds, in the buffer described previously (Sidransky et al., 1991). PCR products were cloned into T-tailed vectors as described (Holton and Graham, 1991) and sequenced with modified T7 polymerase (USB). The insert from one clone (pNP-23) containing human sequences homologous to the yeast MSH2 gene was then used to screen cDNA libraries generated from RNA of SW480 colon cancer cells (Clontech) or of fetal brain (Stratagene). After two further rounds of screening, positive clones were converted into plasmids and sequenced using modified T7 polymerase (Kinzler et al., 1991). In some cases, the inserts were amplified using one Λ SH2-specific primer and one vector- specific primer, and then sequenced with SequiTherm Polymerase (Epicentre Technologies). To determine the 5' end of MSH2 transcripts, RACE was performed (Frohman et al., 1989) using brain and placenta cDNA (Clontech). Example 6 Mutations of hMSH2 The physical mapping of hMSH2 to the ΗNPCC locus was intriguing but could not prove that this gene was responsible for the disease. To obtain more compelling evidence, we determined whether germ line mutations of hMSH2 were present in the two ΗNPCC kindreds that originally established linkage to chromosome 2 (Peltomaki et al., 1993a). Intron-exon borders within the most conserved region of hMSH2 (Figure 6) were determined by sequencing genomic PCR fragments containing adjacent exons. Genomic DNA samples from the lymphocytes of affected members of these two kindreds were then used as SUBSTITUTE SHEET (BULE 26) templates for PCR to determine the sequence of this domain. The DNA from individual J-42, afflicted with colon and endometrial cancer at ages 42 and 44, respectively, was found to contain one allele with a C to T transition at codon 622 (CCA to CTA), resulting in a substitution of leucine for proline (Figure 7, top). Twenty additional DNA samples from unrelated individuals all encoded proline at this position. Twenty one members of the J kindred were then analyzed by direct sequencing of PCR products. All eleven affected individuals contained one allele with a C to T transition in codon 622, while all ten unaffected members contained two normal alleles, thus documenting perfect segregation with disease. Importantly, this proline was at a highly conserved position, the identical residue being found in all known MutS related genes from prokaryotes and eukaryotes (Figure 6 and Reenan and Kolodner, 1992). No mutations of the conserved region of MSH2 were identified in kindred C, so we next examined other parts of the HMSH2 transcript. RNA was purified from lymphoblastoid cells of patient C-202, a 27 year old male with colon cancer. Reverse transcriptase coupled PCR (RT-PCR) was used to generate four hMSH2- specific products encompassing codons 89 to 934 from this RNA (see Experimental Procedures). An abnormal, smaller RT-PCR product was identified with one of the primer pairs used. Mapping and sequencing studies using various MSH2 primers showed that the abnormal product was the result of a presumptive splicing defect which removed codons 265 to 314 from the hMSH2 transcript. The abnormal transcript was found to segregate with disease in the C kindred, and was not found in twenty unrelated individuals. We next wished to determine whether hMSH2 was altered in one of the more recently linked families (R.P. and M.N-L., unpublished data) , and chose kindred 8 for detailed analysis. DNA and RNA were obtained from lymphoblastoid cells of 8-143, a 42 year old male with colon cancer. The conserved region of hMSH2 was amplified from genomic DNA using PCR and directly sequenced. A T to C substitution was noted in the polypyrimidine tract upstream of the exon beginning at codon 669 (at intron position -6). However, this substitution was also found in two of twenty unrelated, normal individuals, and was therefore a polymorphism unrelated to the disease, with an allele frequency of 0.05. Most of the hMSH2 coding region was then amplified by RT-PCR, as described above, and no abnormal transcripts were detected. Sequencing of the PCR products, however, revealed a C to T transition at codon 406 (CGA to TGA) causing substitution of a termination codon for an arginine residue. RNA was available from the lymphocytes of a second affected member of kindred 8, and the same stop codon was identified. This alteration was not found in twenty other, unrelated individuals. Finally, we wished to determine whether mutations of this gene occurred in RER + tumors from patients without evident family histories of cancer. The conserved region of MSH2 was studied in four colorectal tumor cell lines from such patients using genomic DNA as templates for PCR. One tumor (from patient CxlO) was found to contain two hMSH2 alterations. The first was a C to T transition in codon 639 (CAT to TAT), resulting in a substitution of tyrosine for histidine. This change was not found in any of twenty samples from unrelated individuals, but was present in the DNA from normal colon of this patient, and was therefore likely to represent a germ line change (Figure 7, middle). Like the missense mutation in the J kindred, the CxlO alteration was at a position perfectly conserved in all MutS homologs (Figure 6 and Reenan and Kolodner, 1992). The second alteration in the tumor from CxlO was a substitution of a GT dinucleotide for an A in codon 663 (ATG to TGTG). The resultant one bp insertion was predicted to cause a frameshift, producing a termination codon 36 nt downstream. This mutation was demonstrated in both RNA and DNA purified from the CxlO tumor, but was not present in the patient's normal colon, so represented a somatic mutation (Figure 7, bottom). The PCR products from CxlO were cloned and sequenced, and the insertion mutation at codon 663 and the transition at codon 639 were shown to reside on different alleles. Methods To detect mutations, PCR products were generated from cDNA and human genomic DNA templates, then sequenced directly using SequiTherm™. In some cases, the PCR products were cloned into T-tailed vectors for sequencing to confirm the direct sequencing data. The primers used to amplify the conserved region of MSH2 from genomic DNA were 5' -CCA CAA TGG AC A CTT CTG C-3' and 5'-CAC CTG TTC CAT ATG TAC G-3', resulting in a 1.4 kb fragment containing hMSH2 codons 614 to 705, and primers 5'-AAA ATG GGT TGC AAA CAT GC-3' and 5'-GTG ATA GTA CTC ATG GCC C-3', resulting in a 2.0 kb fragment containing MSH2 cDNA codons 683 to 783. Primers for RT-PCR were 5'-AGA TCT TCT TCT GGT TCG TC-3' and 5'-GCC AAC AAT AAT TTC TGG TG-3' for codons 89 to 433, 5'-TGG ATA AGA AC A GAA TAG AGG-3' and 5'-CCA CAA TGG ACA CTT CTG C-3' for codons 350-705, 5'-CAC CTG TTC CAT ATG TAC G-3' and 5'-AAA ATG GGT TGC AAA CAT GC-3' for codons 614 to 783, and 5'-GTG ATA GTA CTC ATG GCC C-3' and 5'-GAC AAT AGC TTA TCA ATA TTA CC-3' for codons 683-949. Discussion Three major conclusions can be drawn from the examples described here. First, physical mapping and linkage analysis localized the HNPCC locus on chromosome 2 to an 0.8 Mb segment bordered by markers CA5 and ze3. Second, a new human homolog of the yeast MSH2 gene was identified, and this gene shown to lie in the same 0.8 Mb interval. Third, alterations of the hMSH2 gene occurred in the germ line of patients with RER + tumors, with or without classical HNPCC, and additional somatic alterations of this gene occurred in tumors (Summarized in Table I). The alterations were at highly conserved regions or significantly altered the expected gene product and thus represent mutations with important functional effects. These results indicate that mutations of hMSH2 are responsible for HNPCC and the RER + positive phenotype found in tumors. These data have substantial implications for understanding the neoplastic disease observed in HNPCC. In particular, they suggest that the microsatellite alterations previously observed in tumors from these patients are not epiphenomena, but are intrinsically related to pathogenesis. Additionally, the mutations observed in yeast and bacteria with defective MutS-related genes are not confined to insertions and deletions at simple repeated sequences, though these sequences provide convenient tools for analysis (Modrich, 1991). Similarly, one would expect that many mutations, in addition to microsatellite insertions or deletions, would be found in HNPCC tumors. This could lead to the multiple, sequential mutations in oncogenes and tumor suppressor genes which have been shown to drive colorectal tumorigenesis (Fearon and Vogelstein, 1990). Thus, the molecular pathogenesis of HNPCC tumors is likely to be similar to that occurring in non-HNPCC cases, though accelerated by the increased rate of mutation associated with mismatch repair defects. Accordingly, colon tumors from HNPCC patients have been shown to contain mutations of APC, p53 and RAS at frequencies similar to those found in sporadic colorectal cancers (Aaltonen et al., 1993). Colorectal tumors from HNPCC patients are distinguished by their relatively normal cytogenetic composition (Kouri et al., 1990), and sporadic, RER + tumors have been demonstrated to have substantially fewer chromosome losses than those occurring in RER" cases (Thibodeau et al., 1993; Aaltonen et al., 1993). These data suggest that genetic heterogeneity is critical for colorectal cancer development, but can be generated in two different ways (Thibodeau et al., 1993). Most commonly, it develops through gross alterations resulting in aneuploidy, as suggested nearly eighty years ago (Boveri, 1914). In HNPCC- derived tumors and RER + sporadic tumors, the diversity is presumably more subtle, consisting of multiple small sequence changes distributed throughout the genome. 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Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. American Journal of Human Genetics 44, 338-396. Weissenbach, J., Gyapay, G., Dib, C, Vignal A., Morissette, J., Millasseau, P., Vaysseix, G., and Lathrop, M. (1992). A second-generation linkage map of the human genome. Nature 359, 794-801. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Vogelstein, Bert Kinzler, Kenneth W. de la Chappelle, Albert (ii) TITLE OF INVENTION: Mutator Gene and Hereditary Non-Polyposis Colorectal Cancer (iii) NUMBER OF SEQUENCES: 16 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Banner, Birch, McKie, and Beckett (B) STREET: 1001 G Street, N.W. (C) CITY: Washington (D) STATE: D.C. (E) COUNTRY: USA (F) ZIP: 20001 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: US 08/160295 (B) FILING DATE: 02-DEC-1993 (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Kagan, Sarah A. (B) REGISTRATION NUMBER: 32,141 (C) REFERENCE/DOCKET NUMBER: 01107.44900 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 202.508.9100 (B) TELEFAX: 202.508.9299 (C) TELEX: 197430 BBMB UT (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2947 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (xi) SEQUENCE DESCRIPTION: SEQ ID N0:1: GGCGGGAAAC AGCTTAGTGG GTGTGGGGTC GCGCATTTTC TTCAACCAGG AGGTGAGGAG 60 GTTTCGACAT GGCGGTGCAG CCGAAGGAGA CGCTGCAGTT GGAGAGCGCG GCCGAGGTCG 120 GCTTCGTGCG CTTCTTTCAG GGCATGCCGG AGAAGCCGAC CACCACAGTG CGCCTTTTCG 180 ACCGGGGCGA CTTCTATACG GCGCACGGCG AGGACGCGCT GCTGGCCGCC CGGGAGGTGT 240 TCAAGACCCA GGGGGTGATC AAGTACATGG GGCCGGCAGG AGCAAAGAAT CTGCAGAGTG 300 TTGTGCTTAG TAAAATGAAT TTTGAATCTT TTGTAAAAGA TCTTCTTCTG GTTCGTCAGT 360 ATAGAGTTGA AGTTTATAAG AATAGAGCTG GAAATAAGGC ATCCAAGGAG AATGATTGGT 420 ATTTGGCATA TAAGGCTTCT CCTGGCAATC TCTCTCAGTT TGAAGACATT CTCTTTGGTA 480 ACAATGATAT GTCAGCTTCC ATTGGTGTTG TGGGTGTTAA AATGTCCGCA GTTGATGGCC 540 AGAGACAGGT TGGAGTTGGG TATGTGGATT CCATACAGAG GAAACTAGGA CTGTGTGAAT 600 TCCCTGATAA TGATCAGTTC TCCAATCTTG AGGCTCTCCT CATCCAGATT GGACCAAAGG 660 AATGTGTTTT ACCCGGAGGA GAGACTGCTG GAGACATGGG GAAACTGAGA CAGATAATTC 720 AAAGAGGAGG AATTCTGATC ACAGAAAGAA AAAAAGCTGA CTTTTCCACA AAAGACATTT 780 ATCAGGACCT CAACCGGTTG TTGAAAGGCA AAAAGGGAGA GCAGATGAAT AGTGCTGTAT 840 TGCCAGAAAT GGAGAATCAG GTTGCAGTTT CATCACTGTC TGCGGTAATC AAGTTTTTAG 900 AACTCTTATC AGATGATTCC AACTTTGGAC AGTTTGAACT GACTACTTTT GACTTCAGCC 960 AGTATATGAA ATTGGATATT GCAGCAGTCA GAGCCCTTAA CCTTTTTCAG GGTTCTGTTG 1020 AAGATACCAC TGGCTCTCAG TCTCTGGCTG CCTTGCTGAA TAAGTGTAAA ACCCCTCAAG 1080 GACAAAGACT TGTTAACCAG TGGATTAAGC AGCCTCTCAT GGATAAGAAC AGAATAGAGG 1140 AGAGATTGAA TTTAGTGGAA GCTTTTGTAG AAGATGCAGA ATTGAGGCAG ACTTTACAAG 1200 AAGATTTACT TCGTCGATTC CCAGATCTTA ACCGACTTGC CAAGAAGTTT CAAAGACAAG 1260 CAGCAAACTT ACAAGATTGT TACCGACTCT ATCAGGGTAT AAATCAACTA CCTAATGTTA 1320 TACAGGCTCT GGAAAAACAT GAAGGAAAAC ACCAGAAATT ATTGTTGGCA GTTTTTGTGA 1380 CTCCTCTTAC TGATCTTCGT TCTGACTTCT CCAAGTTTCA GGAAATGATA GAAACAACTT 1440 TAGATATGGA TCAGGTGGAA AACCATGAAT TCCTTGTAAA ACCTTCATTT GATCCTAATC 1500 TCAGTGAATT AAGAGAAATA ATGAATGACT TGGAAAAGAA GATGCAGTCA ACATTAATAA 1560 GTGCAGCCAG AGATCTTGGC TTGGACCCTG GCAAACAGAT TAAACTGGAT TCCAGTGCAC 1620 AGTTTGGATA TTACTTTCGT GTAACCTGTA AGGAAGAAAA AGTCCTTCGT AACAATAAAA 1680 ACTTTAGTAC TGTAGATATC CAGAAGAATG GTGTTAAATT TACCAACAGC AAATTGACTT 1740 CTTTAAATGA AGAGTATACC AAAAATAAAA CAGAATATGA AGAAGCCCAG GATGCCATTG 1800 TTAAAGAAAT TGTCAATATT TCTTCAGGCT ATGTAGAACC TGTTAGCTCA GCTAGATGCT GTTGTCAGCT TTGCTCACGT CATATGTACG ACCAGCCATT TTGGAGAAAG GACAAGGAAG GGCATGCTTG TGTTGAAGTT CAAGATGAAA TTGCATTTAT AAAAAGATAA ACAGATGTTC CACATCATTA CTGGCCCCAA ATATTCGACA AACTGGGGTG ATAGTACTCA TGGCCCAAAT AGTCAGCAGA AGTGTCCATT GTGGACTGCA TCTTAGCCCG AATTGAAAGG AGTCTCCACG TTCATGGCTG AAATGTTGGA CTGCAACCAA AGATTCATTA ATAATCATAG ATGAATTGGG ATGGATTTGG GTTAGCATGG GCTATATCAG AATACATTGC GCATGTTTGC AACCCATTTT CATGAACTTA CTGCCTTGGC ATAATCTACA TGTCACAGCA CTCACCACTG AAGAGACCTT AGAAAGGTGT CTGTGATCAA AGTTTTGGGA TTCATGTTGC AGCATGTAAT AGAGTGTGCT AAACAGAAAG CCCTGGAACT GAGAATCGCA AGGATATGAT ATCATGGAAC CAGCAGCAAA AGCAAGGTGA AAAAATTATT CAGGAGTTCC TGTCCAAGGT AAATGTCAGA AGAAAACATC ACAATAAAGT TAAAACAGCT AGAATAATAG CTTTGTAAAT GAAATCATTT CACGAATAAA CAGTAATGGA ATGAAGGTAA TATTGATAAG CTATTGTCTG ATATTAA (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 934 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: YES (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: Met Ala Val Gin Pro Lys Glu Thr Leu Gin Leu Glu Ser Ala Ala Glu 1 5 10 15 Val Gly Phe Val Arg Phe Phe Gin Gly Met Pro Glu Lys Pro Thr Thr 20 25 30 Thr Val Arg Leu Phe Asp Arg Gly Asp Phe Tyr Thr Ala His Gly Glu 35 40 45 Asp Ala Leu Leu Ala Ala Arg Glu Val Phe Lys Thr Gin Gly Val lie 50 55 60 Lys Tyr Met Gly Pro Ala Gly Ala Lys Asn Leu Gin Ser Val Val Leu 65 70 75 80 Ser Lys Met Asn Phe Glu Ser Phe Val Lys Asp Leu Leu Leu Val Arg 85 90 95 Gin Tyr Arg Val Glu Val Tyr Lys Asn Arg Ala Gly Asn Lys Ala Ser 100 105 110 Lys Glu Asn Asp Trp Tyr Leu Ala Tyr Lys Ala Ser Pro Gly Asn Leu 115 120 125 Ser Gin Phe Glu Asp lie Leu Phe Gly Asn Asn Asp Met Ser Ala Ser 130 135 140 He Gly Val Val Gly Val Lys Met Ser Ala Val Asp Gly Gin Arg Gin 145 150 155 160 Val Gly Val Gly Tyr Val Asp Ser He Gin Arg Lys Leu Gly Leu Cys 165 170 175 Glu Phe Pro Asp Asn Asp Gin Phe Ser Asn Leu Glu Ala Leu Leu He 180 185 190 Gin He Gly Pro Lys Glu Cys Val Leu Pro Gly Gly Glu Thr Ala Gly 195 200 205 Asp Met Gly Lys Leu Arg Gin He He Gin Arg Gly Gly He Leu He 210 215 220 Thr Glu Arg Lys Lys Ala Asp Phe Ser Thr Lys Asp He Tyr Gin Asp 225 230 235 240 Leu Asn Arg Leu Leu Lys Gly Lys Lys Gly Glu Gin Met Asn Ser Ala 245 250 255 Val Leu Pro Glu Met Glu Asn Gin Val Ala Val Ser Ser Leu Ser Ala 260 265 270 Val He Lys Phe Leu Glu Leu Leu Ser Asp Asp Ser Asn Phe Gly Gin 275 280 285 Phe Glu Leu Thr Thr Phe Asp Phe Ser Gin Tyr Met Lys Leu Asp He 290 295 300 Ala Ala Val Arg Ala Leu Asn Leu Phe Gin Gly Ser Val Glu Asp Thr 305 310 315 320 Thr Gly Ser Gin Ser Leu Ala Ala Leu Leu Asn Lys Cys Lys Thr Pro 325 330 335 Gin Gly Gin Arg Leu Val Asn Gin Trp He Lys Gin Pro Leu Met Asp 340 345 350 Lys Asn Arg He Glu Glu Arg Leu Asn Leu Val Glu Ala Phe Val Glu 355 360 365 Asp Ala Glu Leu Arg Gin Thr Leu Gin Glu Asp Leu Leu Arg Arg Phe 370 375 380 Pro Asp Leu Asn Arg Leu Ala Lys Lys Phe Gin Arg Gin Ala Ala Asn 385 390 395 400 Leu Gin Asp Cys Tyr Arg Leu Tyr Gin Gly He Asn Gin Leu Pro Asn 405 410 415 Val He Gin Ala Leu Glu Lys His Glu Gly Lys His Gin Lys Leu Leu 420 425 430 Leu Ala Val Phe Val Thr Pro Leu Thr Asp Leu Arg Ser Asp Phe Ser 435 440 445 Lys Phe Gin Glu Met He Glu Thr Thr Leu Asp Met Asp Gin Val Glu 450 455 460 Asn His Glu Phe Leu Val Lys Pro Ser Phe Asp Pro Asn Leu Ser Glu 465 470 475 480 Leu Arg Glu He Met Asn Asp Leu Glu Lys Lys Met Gin Ser Thr Leu 485 490 495 He Ser Ala Ala Arg Asp Leu Gly Leu Asp Pro Gly Lys Gin He Lys 500 505 510 Leu Asp Ser Ser Ala Gin Phe Gly Tyr Tyr Phe Arg Val Thr Cys Lys 515 520 525 Glu Glu Lys Val Leu Arg Asn Asn Lys Asn Phe Ser Thr Val Asp He 530 535 540 Gin Lys Asn Gly Val Lys Phe Thr Asn Ser Lys Leu Thr Ser Leu Asn 545 550 555 560 Glu Glu Tyr Thr Lys Asn Lys Thr Glu Tyr Glu Glu Ala Gin Asp Ala 565 570 575 He Val Lys Glu He Val Asn He Ser Ser Gly Tyr Val Glu Pro Met 580 585 590 Gin Thr Leu Asn Asp Val Leu Ala Gin Leu Asp Ala Val Val Ser Phe 595 600 605 Ala His Val Ser Asn Gly Ala Pro Val Pro Tyr Val Arg Pro Ala He 610 615 620 Leu Glu Lys Gly Gin Gly Arg He He Leu Lys Ala Ser Arg His Ala 625 630 635 640 Cys Val Glu Val Gin Asp Glu He Ala Phe He Pro Asn Asp Val Tyr 645 650 655 Phe Glu Lys Asp Lys Gin Met Phe His He He Thr Gly Pro Asn Met 660 665 670 Gly Gly Lys Ser Thr Tyr He Arg Gin Thr Gly Val He Val Leu Met 675 680 685 Ala Gin He Gly Cys Phe Val Pro Cys Glu Ser Ala Glu Val Ser He 690 695 700 Val Asp Cys He Leu Ala Arg Val Gly Ala Gly Asp Ser Gin Leu Lys 705 710 715 720 Gly Val Ser Thr Phe Met Ala Glu Met Leu Glu Thr Ala Ser He Leu 725 730 735 Arg Ser Ala Thr Lys Asp Ser Leu He He He Asp Glu Leu Gly Arg 740 745 750 Gly Thr Ser Thr Tyr Asp Gly Phe Gly Leu Ala Trp Ala He Ser Glu 755 760 765 Tyr He Ala Thr Lys He Gly Ala Phe Cys Met Phe Ala Thr His Phe 770 775 780 His Glu Leu Thr Ala Leu Ala Asn Gin He Pro Thr Val Asn Asn Leu 785 790 795 800 His Val Thr Ala Leu Thr Thr Glu Glu Thr Leu Thr Met Leu Tyr Gin 805 810 815 Val Lys Lys Gly Val Cys Asp Gin Ser Phe Gly He His Val Ala Glu 820 825 830 Leu Ala Asn Phe Pro Lys His Val He Glu Cys Ala Lys Gin Lys Ala 835 840 845 Leu Glu Leu Glu Glu Phe Gin Tyr He Gly Glu Ser Gin Gly Tyr Asp 850 855 860 He Met Glu Pro Ala Ala Lys Lys Cys Tyr Leu Glu Arg Glu Gin Gly 865 870 875 880 Glu Lys He He Gin Glu Phe Leu Ser Lys Val Lys Gin Met Pro Phe 885 890 895 Thr Glu Met Ser Glu Glu Asn He Thr He Lys Leu Lys Gin Leu Lys 900 905 910 Ala Glu Val He Ala Lys Asn Asn Ser Phe Val Asn Glu He He Ser 915 920 925 Arg He Lys Val Thr Thr 930 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: CTGGATCCAC NGGNCCNAAY ATG 23 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: CTGGATCCRT ARTGNGTNRC RAA 23 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: CCACAATGGA CACTTCTGC 19 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: CACCTGTTCC ATATGTACG 19 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: AAAATGGGTT GCAAACATGC 20 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: GTGATAGTAC TCATGGCCC 19 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: AGATCTTCTT CTGGTTCGTC 20 (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: GCCAACAATA ATTTCTGGTG 20 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: TGGATAAGAA CAGAATAGAG G 21 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: CCACAATGGA CACTTCTGC 19 (2) INFORMATION FOR SEQ ID NO:13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: CACCTGTTCC ATATGTACG 19 (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: AAAATGGGTT GCAAACATGC 20 (2) INFORMATION FOR SEQ ID NO:15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: GTGATAGTAC TCATGGCCC 19 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iv) ANTI -SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: GACAATAGCT TATCAATATT ACC 23

Claims: CLABMS 1. An isolated and purified DNA molecule having a sequence of at least about 20 nucleotides of hMSH2, as shown in SEQ ID NO:l. 2. The DNA molecule of claim 1 which is cDNA. 3. The DNA molecule of claim 1 which is labeled. 4. The DNA molecule of claim 1 which is operably linked to a promotor sequence. 5. The DNA molecule of claim 4 which upon transcription produces an RNA molecule having the sequence of native hMSH2 mRNA. 6. An isolated and purified DNA molecule having a sequence of at least about 20 nucleotides of an HMSH2 allele found in a tumor wherein said DNA molecule contains a mutation relative to hMSH2 shown in SEQ ID NO:l. 7. A method of treating a person predisposed to hereditary non- polyposis colorectal cancer to prevent accumulation of somatic mutations, comprising: administering a DNA molecule which has a sequence of at least about 20 nucleotides of hMSH2, as shown in SEQ ID NO:l, to a person having a mutation in an hMSH2 allele which predisposes the person to hereditary non- polyposis colorectal cancer, wherein said DNA molecule is sufficient to remedy the mutation in an hMSH2 allele of the person. 8. The method of claim 7 wherein said DNA molecule encodes an h SH2 protein as shown in SEQ ID NO:l. 9. The method of claim 7 wherein said DNA molecule spans the mutation site in said allele and remedies the mutation by recombining with said mutant allele. 10. The method of claim 7 wherein said DNA molecule encodes a portion of MSH2 protein which is sufficient to provide DNA mismatch repair function. 11. A method of determining a predisposition to cancer comprising: testing a body sample of a human to ascertan the presence of a mutation in hMSH2 which affects HMSH2 expression or hMSH2 protein function, the presence of such a mutation indicating a predisposition to cancer. 12. The method of claim 11 wherein the sample is DNA. 13. The method of claim 11 wherein the sample is RNA. 14. The method of claim 11 wherein the sample is protein. 15. The method of claim 11 wherein the sample is isolated from prenatal or embryonic cells. 16. A method of screening to identify therapeutic agents which can prevent or ameliorate tumors, comprising: contacting a test compound with a cell; determining the ability of the cell to perform DNA mismatch repair, a test compound which increases the ability of said cell to perform DNA mismatch repair being a potential therapeutic agent. 17. The method of claim 16 wherein the cell contains an hMSH2 mutation found in a tumor. 18. A method of screening to identify therapeutic agents which can prevent or ameliorate tumors, comprising: contacting a test compound with an isolated and purfied hMSH2 protein; determining the ability of the hMSB.2 protein to perform DNA mismatch repair, a test compound which increases the ability of said protein to perform DNA mismatch repair being a potential therapeutic agent. 19. The method of claim 18 wherein the hMSH2 protein contains a mutation found in a tumor. 20. An isolated and purified protein which has the sequence shown in SEQ ID NO:2. 21. A transgenic animal wherein the transgene is an hMSH2 allele. 22. The transgenic animal of claim 21 wherein the allele is a mutant allele of hMSH2 found in a patient having hereditary nonpolyposis colorectal cancer or an RER + tumor. 23. A non-human animal which contains no wild-type MSH2 allele. 24. The transgenic animal of claim 21 wherein said animal contains no wild-type MSH2 allele. 25. A method of treating a person having an RER + tumor to prevent accumulation of somatic mutations leading to resistance to an anti-cancer therapeutic agent, comprising: administering to a person having a tumor with an RER + phenotype: (a) a DNA molecule which has a sequence of at least about 20 nucleotides of hMSH2, as shown in SEQ ID NO:l, wherein said DNA molecule is sufficient to remedy a mutation in an hMSH2 allele of the person; and (b) an anti-neoplastic therapeutic agent. 26. The method of claim 23 wherein the anti-neoplastic therapeutic agent is selected from the group consisting of: a hormone, radiation, a cytotoxic drug, a cytotoxin, and an antibody.

Inventors: DE LA CHAPELLE ALBERT, KINZLER KENNETH W, VOGELSTEIN BERT, DE LA CHAPELLE, ALBERT, KINZLER, KENNETH, W., VOGELSTEIN, BERT
Applicants: CHAPELLE ALBERT DE, UNIV JOHNS HOPKINS, DE LA CHAPELLE, ALBERT, THE JOHNS HOPKINS UNIVERSITY
Application year: 1994
Main classification: C12N15
IPC codes: A01K67, A61K31, A61K38, A61K39, A61K45, A61K48, A61P35, A61P43, C07H21, C07K14, C12N15, C12Q1, G01N33