Hepatobiliary magnetic resonance contrast agents

Abstract: A compound of the formula: wherein R 1 and R 2 are both hydrogen and R 3 and R 4 together with the carbon atoms in the tetraazacyclododecane macrocycle to which they are attached form a fused fully or partially saturated non aromatic cyclohexyl ring which may be unsubstituted or substituted by one or more halogen, straight or branched (C 1 -C 5 )alkyl, alkyl oxide of formula -R-OR wherein each R is a straight or branched (C 1 -C 5 )alkyl, hydroxy or hydroxyalkyl groups, and which may be further fused to a phenyl or cyclohexyl ring p is 0 or 1 and R 6 is hydrogen or a straight or branched (C 1 -C 5 ) alkyl group; or a salt thereof and the metal chelates thereof, particularly gadolinium chelates, the pharmaceutical and diagnostic compositions containing them and their use in MRI.

Description: Brief Description of the Invention In accordance with the present invention, novel compounds are provided. These compounds are useful, for example, as metal-chelating ligands and, in the form of metal complexes, the present compounds are especially useful as diagnostic contrast agents. When the metal in the complex is paramagnetic, the diagnostic contrast agents are suitable for magnetic resonance imaging, and are particularly useful for magnetic resonance imaging (MRI)of the liver and bile ducts. The compounds of the invention comprise a tetraazacyclododecane macrocycle containing at least one fused cyclohexyl ring, and are represented by the formula I: wherein R 1 and R 2 together with the carbon atoms in the tetraazacyclododecane macrocycle to which they attached form a fused fully or partially saturated nonaromatic six membered carbocyclic ring which may be unsubstituted or substituted by one or more halogen, straight or branched (C 1 -C 5 ) alkyl, hydroxy or hydroxyalkyl groups, alkyl oxide of formula -R-OR wherein each R is a straight or branched (C 1 -C 5 )akyl and which may be further fused to a phenyl or cyclohexyl ring, p is 0 or 1 and R 3 is hydrogen or straight or branched (C 1 -C 5 )alkyl group; or a salt thereof. Detailed Description of the Invention Listed below are definitions of various terms used in the description of this invention. These definitions apply to the terms as they are used throughout this specification, unless otherwise limited in specific instances, either individually or as part of a larger group. The terms "alkyl" and "alkoxy" refer to both straight and branched, unsubstituted chains of carbon atoms. Those chains having 1 to 5 carbon atoms are preferred. Methyl and methoxy are the most preferred alkyl and alkoxy groups, respectively. "Hydroxyalkyl" refers to straight and branched alkyl groups including one or more hydroxy radicals such as -CH 2 CH 2 OH, -CH 2 CH 2 OHCH 2 OH, CH(CH 2 OH) 2 and the like. (See, for example, Sovak, M., Editor, Radiocontrast Agents, Springer-Verlag, 1984, pp. 1-125). The term "carbocyclic ring" refers to a ring system in which all the ring atoms are carbon, e.g., phenyl or cyclohexyl. The ring may be unsubstituted or substituted by, for example, alkyl, halogen, hydroxy, alkoxy, and the like. The term "halogen" refers to bromo, chloro, fluoro or iodo. The term "carboxy" refers to the group -C(O)OH or the group -C(O)OR where R is alkyl. Preferred compounds of the present invention are those wherein R 1 and R 2 form a fused fully saturated cyclohexyl ring, R 3 is -H and p is zero. The compounds of formula I, and salts thereof, may be complexed with a paramagnetic metal atom and used as relaxation enhancement agents for magnetic resonance imaging. These agents, when administered to a mammalian host (e.g., a human) distribute in various concentrations to different tissues, and catalyze relaxation of protons (in the tissues) that have been excited by the absorption of radiofrequency energy from a magnetic resonance imager. This acceleration of the rate of relaxation of the excited protons provides for an image of different contrast when the host is scanned with a magnetic resonance imager. The magnetic resonance imager is used to record images at various times, generally either before and after administration of the agents, or after administration only, and the differences in the images created by the agents' presence in tissues are used in diagnosis. In proton magnetic resonance imaging, paramagnetic metal atoms such as gadolinium (III), and manganese(II), chromium(III) and iron(III) (all are paramagnetic metal atoms with favorable electronic properties) are preferred as metals complexed by the ligands of formula I. Gadolinium(III) is the most preferred complexed metal due to the fact that it has the highest paramagnetism, it has low toxicity when complexed to a suitable ligand, and it has high lability of coordinated water. The mecal-chelacing ligands of the present invention can be complexed with a lanthanide (atomic number 58 to 71) and used as chemical shift agents in magnetic resonance imaging or in magnetic resonance in vivo spectroscopy. Paramagnetic metal complexes of the present invention are particularly useful as hepatobiliary agents, i.e., for magnetic resonance imaging of the liver and bile ducts. While the above-described uses for the metal-chelating ligands of the present invention are preferred, those working in the diagnostic arts will appreciate that the ligands can also be complexed with the appropriate metals and used as contrast agents in other imaging cechniques such as x-ray imaging, radionuclide imaging and ultrasound imaging, and in radiotherapy. Use in Imaging To use the ligands of the present invention for imaging, they are first complexed with an appropriate metal. This may be accomplished by methodology known in the art. For example, the metal can be added to water in the form of an oxide or in the form of a halide or acetate and treated with an equimolar amount of a ligand of the present invention. The ligand can be added as an aqueous solution or suspension. Dilute acid or base can be added (where appropriate) to maintain a suitable pH. Heating at temperatures as high as 100°C for periods of up to 24 hours or more may sometimes be employed to facilitate complexation, depending on the metal and the chelator, and their concentrations. Pharmaceutically acceptable salts of the metal complexes of the ligands of this invention are also useful as imaging agents. They can be prepared by using a base (e.g., an alkali metal hydroxide, meglumine, arginine or lysine) to neutralize the above-prepared metal complexes while they are still in solution. Some of the metal complexes are formally uncharged and do not need cations as counterions. Such neutral complexes may be preferred as intravenously administered x-ray and NMR imaging agents over charged complexes because they may provide solutions of greater physiologic tolerance due to their lower osmolality. However, for use as hepacobiliary agents, negatively charged ligands are preferred. The present invention provides pharmaceutical compositions comprising a compound of formula I, or a salt thereof, optionally complexed with a metal, and a pharmaceutically acceptable vehicle or diluent. The present invention further provides a method for diagnostic imaging comprising the steps of administering to a host a compound of the formula I, or a salt thereof, which is complexed with a metal, and obtaining a diagnostic image, preferably a magnetic resonance image, of said host. Sterile aqueous solutions of the chelate complexes of the present invention are preferably administered to mammals (e.g., humans) orally, intrathecally and, especially, intravenously in concentrations of 0.003 to 1.0 molar. Use of the metal complexes of the present invention as hepatobiliary agents is preferred. For example, for visualization of the liver, the dose is preferably 0.03 to 0.3 millimole/kilogram. While visualization of the liver and bile ducts is preferred, the metal complexes of the present invention may be employed for visualization of other sites. For example, for the visualization of brain lesions using magnetic resonance imaging, a gadolinium complex of a ligand of formula I may be administered intravenously at a dose of 0.05 to 0.5 millimoles of the complex per kilogram of body weight, preferably at a dose of 0.1 to 0.3 millimoles/kilogram. For visualization of the kidneys, the dose is preferably 0.05 to 0.20 millimoles/kilogram. For visualization of the heart, the dose is preferably 0.05 to 0.3 millimoles/kilogram. The pH of the formulation of the present metal complexes is preferably between about 6.0 and 8.0, most preferably between about 6.5 and 7.5. Physiologically acceptable buffers (e.g., tris(hydroxymethyl)-aminomethane) and other physiologically acceptable additives (e.g., stabilizers such as parabens) may also be present. It is also advantageous to employ dual scavenging excipients such as those described in copending application U.S. Serial No. 032,763, filed March 15, 1993, entitled "DUAL FUNCTIONING EXCIPIENT FOR METAL CHELATE CONTRAST AGENTS", incorporated herein by reference. Those excipients have a general formula corresponding to: D s [D'(L')] t wherein D and D' are independently Ca or Zn, L' is an organic ligand which may be different from, or the same as, the ligand employed to complex the metal, and s and t are independently 1, 2 or 3. The compounds of formula I can be prepared according to the following general scheme: All stereoisomers of the compounds and complexes of the present invention are contemplated herein, whether alone (that is, substantially free of other isomers), in a mixture of certain stereoisomers (for example, as a racemate) or in any other mixture thereof. The invention will now be further described by the following examples. These examples are illustrative rather than limiting. Example 1 2,5,8,11-Tetracarboxymethyl-2,5,8,11-tetraazabicyclo[10.4.0]hexadecane (A) 4,9-dioxo-2,5,8,11-tetraaza-bicyclo[10,4,0]-hexadecane Sodium carbonate (226 g., 2.13 mol.) and trans-1,2-diaminocyclohexane (14.1 ml., 117 mmol.) were added to a solution of N,N'-dichloroacetylethylenediamine (25 g., 117 mmol.) in dry acetonitrile (3.75 1.) under nitrogen. N,N'-dichloroacetylethylenediamine was prepared as reported in "New multidentate potential ionophors of ether-amide type", Monat. Chem. , 1985, 116 , 217-221. The reaction mixture was refluxed for 20 hours. The insoluble material formed was filtered off and the volume of the filtrate was reduced to 1 l. by evaporation. Crystals of the monomer slowly formed at room temperature. They were collected by filtration and the product was purified by crystallization in acetonitrile. The yield was 7.43 g. of a white solid. The acetonitrile solution obtained after filtration of compound (A) was concentrated and several fractions of crystallization were collected. The mass spectrum (FAB) and the 1 H and 13 C NMR spectra of each fraction was recorded and the fractions containing primarily the same compound were combined. Each fraction was crystallized twice from acetonitrile. (B) 2,5,8,11-tetraaza-bicyclo[10.4.0]hexadecane Dried compound (A) (2.54 g., 10 mmol.: was added under nitrogen to a 1 M solution of BH 3 in tetrahydrofuran (100 ml., 100 mmol.). The reaction mixture was refluxed overnight. After cooling, water was added dropwise until the excess of BH 3 was completely eliminated. The resulting suspension was brought to dryness in a rotary evaporator and the remaining solid was added to 140 ml. of a 6 M hydrochloric acid solution. The temperature was maintained at 100° C. overnight. Water was eliminated under vacuum and the solid residue was dissolved in a minimum amount of water. LiOH·H 2 O was added to give a pH adjusted to 12 - 13. The mixture was extracted three times with methylene chloride. The combined organic fractions were dried (magnesium sulfate), filtered, and evaporated to give 2 g. of compound (B) as a white solid. (C) 2,5,8,11-tetracarboxymethyl-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane Bromoacetic acid (11.9 g., 86 mmol.) was dissolved in water (25 ml.). The temperature of the solution was lowered to 5° C. in an ice bath and the acid was neutralized with an aqueous solution of sodium hydroxide (3.43 g., 86 mmol. in 13 ml. water). Compound (B) (3.24 g., 14.3 mmol.) was added to the solution of sodium bromoacetate and the temperature was raised to 70 - 80° C. The pH was maintained between 9 and 10 by the dropwise addition of a solution of sodium hydroxide (3.43 g., 86 mmol.) in water (13 ml.). At the end of the addition, the temperature was maintained at 70 - 80° C. for 6 hours. The volume of the reaction mixture was then brought down to 40 ml. and the pH was acidified to 3 with 6 M hydrochloric acid. An insoluble material formed rapidly. It was filtered and recrystallized in water until no trace of glycolic acid could be found by NMR. A second fraction of the title compound was obtained after further concentration of the reaction mixture. The two fractions were combined and 3.6 g. of the title compound as a white solid was obtained after recrystallization in water. Mass spectrum (FAB): m/e 459 (M+H) and 481 (M+Na). Example 2 2,5,11-Tricarboxymethyl-8-(2'-hydroxyethyl)-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane (A) 3, 10-dioxo-8- (2' -hydroxyethyl) -2, 5, 8, 11-tetraaza-bicyclo[10,4,0]hexadecane Into a 5 1 erlenmeyer flask containing 5 1 of anhydrous acetonitrile was placed 17.4 g (65 mmol) of N,N'-bis(chloroacetyl)-trans-1,2-diaminocyclohexane (being prepared according to Bull.Chem.Soc.Jpn. ,1974, 47 ,1184-1189),6.8 g (65 nmnol) of 2-(2-aminoethylamino)ethanol and 130 g (1.23 mmol) of sodium carbonate. The reaction mixture was heated at 82°C. under nitrogen for 50 hours. After eliminating sodium carbonate by filtration, acetonitrile was eliminated under vacuum until the volume was reduced to 500 ml. A precipitate formed immediately and the reaction mixture was stirred for several hours at room temperature. The precipitate was filtered off and was washed with a few ml of acetonitrile and dried under vacuum. 8.2 g. of compound (A) was obtained as a white solid. (B) 8-(2'-hydroxyethyl)-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane Into a dry flask under nitrogen was placed 3.00 g. (10 mmol.) of compound (A) and 100 ml. of a 1 M solution of BH 3 ·THF in anhydrous tetrahydrofuran. The reaction mixture was refluxed for 12 hours. Water was added dropwise until no reaction was taking place with the excess of BH 3 ·THF. The solvent was stripped off on a rotary evaporator after addition of a few ml. of methanol to avoid excessive foaming. The solid residue was treated with about 10 ml. of a concentrated solution of LiOF·H 2 O until the pH reached 12 - 13. This aqueous phase was extracted 6 times with 100 ml. of methylene chloride. The extracts were collected and dried over magnesium sulfate. The solvent was removed under vacuum to yield 2.62 g. of Compound (B) as a white powder that was used in the next step without further purification. (C) 2,5,11-tricarboxymethyl-8-(2'-hydroxyethyl)-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane To a solution of 3.19 g. (23 mmol.) of bromoacetic acid in 10 ml. of water chilled in an ice bath was added dropwise 0.92 g. (23 mmol.) of sodium hydroxide dissolved in 5 ml. of water while maintaining the temperature below 5° C. This mixture was added to a solution of 1.38 g. (5.1 mmol.) of compound (B) dissolved in 5 ml, of water. The reaction mixture was heated to 70 - 80° C. and a solution of 0.92 g. of sodium hydroxide (23 mmol.) in 5 ml. of water was added dropwise while maintaining the pH in the 9 - 10 range. After completing the addition of sodium hydroxide, the reaction mixture was agitated 12 additional hours at 70 - 80°C. The reaction mixture was allowed to cool to room temperature and the pH was brought to 3 by addition of a 6 M hydrochloric acid solution. The solution was applied to a cation exchange resin (H + form). After washing with water, the macrocyclic ligand was eluted with 0.5 M aqueous ammonia. Obtained after rotary evaporation was 1.66 g. of the title compound as the ammonium salt. The acid form was obtained by elution on an anion exchange column (formate form) with 0.5 M formic acid. 13 C NMR (D 2 O, strongly acid pH, ppm vs TMS): 177.6, 175.4. 170.4, 67.7, 59.9, 57.9, 57-2, 54.9, 53.4, 52.2, 51.9. 49.9, 48.9, 48.3, 26.0 - 25.6. Mass spectrum (FAB): title compound in the acid form, m/e 445 (M+H). Example 3 2,5,8,11-Tetracarboxymethyl-14,15-benzo-2,5,8,11-tetraaza-bicyclo [10,4,0]hexadecane (A) 4,9-dioxo-14,15-benzo-2,5,8,11-tetraaza-bicyclo[10,4,0]hexadecane Trans-2,3-diamino-1,2,3,4-tetra-hydronaphthalene was prepared as reported by Yano, T., Kobayashi, H. and Ueno, K., "Stereospecific syntheses and acid dissociation of 2,3-diaminotetralins and 2,3-diamino-trans-decalins", Bull. Chem. Soc. Jpn. , 1973 46 , 985 - 990. In a 2 l. flask equipped with a mechanical stirrer and a refluxed condenser were placed 5.38 g. (33.16 mmol.) of trans-2,3-diamino-1,2,3,4-tetrahydronaphthalene and 1 1. of anhydrous acetonitrile. N,N'-dichloroacetylethylenediamine (7.06 g., 33.16 mmol.) and 64 g. (0.60 mol.) of anhydrous sodium carbonate were added to the solution. After 20 hours at 90° C., the reaction mixture was filtered and the filtrate was evaporated until the volume was reduced to 500 ml. A precipitate formed slowly when the solution was allowed to stand at room temperature overnight. It was filtered off and dried under vacuum. The yield was 5.52 g. of a brownish powder. Compound (A) was isolated by adding the powder to 500 ml. of water at 50° C. A brown viscous oil did not dissolve and was eliminated by filtration. The remaining aqueous solution was concentrated under vacuum until white crystals were formed. After letting the solution stand overnight at room temperature, 3.73 g. of compound (A) were collected by filtration. The reaction mixture left after isolation of compound (A) was concentrated further by evaporation of the acetonitrile but it yielded only the dimeric form, m.p. 230 - 231° C. (B) 14,15-benzo-2,5,8,11-tetraaza-bicyclo-[10,4,0]hexadecane The reduction of compound (A) was accomplished by adding 1.5 g. (4.96 mmol.) under a nitrogen sparge to 50 ml. of a 1 M solution of BH 3 ·THF in dry tetrahydrofuran. The reaction mixture was left boiling for 12 hours. The reaction mixture was chilled in an ice bath and water was slowly added until the generation of hydrogen could no longer be observed. The solvent was removed on a rotary evaporator and the remaining solid was suspended in 50 ml. of 6 M hydrochloric acid. The reaction mixture was refluxed overnight. The volatiles were removed on a rotary evaporator. The excess of hydrochloric acid was eliminated by dissolving the remaining solid in water and by evaporating the solution under reduced pressure. This procedure had to be repeated three times. The solid residue obtained after the elimination of the hydrochloric acid in excess was dissolved in 30 ml. of water, and a concentrated solution of LiOB·H 2 O was added until the pH reached 12 - 13. The solution was extracted with 200 ml. of dichloromethane. The organic phase was separated by decantation and was dried (magnesium sulfate), filtered and evaporated to give 1.06 g. of a yellowish vitreous solid. An analytically pure sample was obtained by recrystallization of the tetraamine as the hydrochloride salt. (C) 2,5,8,11-tetracarboxymerhyl-14,15-benzo-2,5,8,11-tetraaza-bicyclo[10,4,0]hexadgcane Bromoacetic acid (7.14 g., 51.4 mmol.) was dissolved in 20 ml. of water and was slowly neutralized by the dropwise addition of a solution of 2.05 g. (51.2 mmol.) of sodium hydroxide in 10 ml. of water while maintaining the temperature below 5° C. The reaction mixture was added to a solution of 2.35 g. (8.57 mmol.) of compound (B) in 10 ml. of water. The temperature was raised to 70 - 80° C. and the pH was maintained between 9 and 10 by the dropwise addition of an aqueous solution of sodium hydroxide (2.05 g.. 51.42 mmol. in 10 ml. of water). The addition of sodium hydroxide was completed after 5 hours and the reaction mixture was left at 70 - 80° C. overnight. After cooling, the volume of the reaction mixture was reduced to 45 ml. on a rotary evaporator and a 6 M solution of hydrochloric acid was added dropwise until the pH was lowered to 3. A beige precipitate (3.5 g.) formed immediately. It was collected by filtration, recrystallized in water (colorless needles) and dried under vacuum. 13 C NMR (D 2 O, strongly basic medium, ppm vs TMS): 182.2, 182.1, 161.9, 161.8, 140.9, 140.3. 129.9, 129.5, 128.8, 128.2, 64.0, 60.7, 59.9, 58.1, 57.3, 56.1. 55.1, 54.9. 51.0, 50.6, 50.4, 46.6, 28.4. Mass spectrum (FAB): m/e 507 (M+H). Example 4 2,5,8,11-Tetracarboxymethyl-2,5,8,11-tetraaza-bicyclo[10,4,0]hexadec-14-ene (A) 4,9-dioxo-2,5,8,11-tetraaza-bicyclo-[10,4,0]hexadec-14-ene the synthesis of trans-4-cyclohexene-1,2-diamine has been reported by Witiak et al. ( J . Med . Chem. 1987, 30 , 1327-1336). Trans-4-cyclohexene-1,2-diamine (9.25 g., 82.5 mmol.) and N,N'-dichloroacethylenediamine (17.55 g., 82.5 mmol.) were dissolved in 2700 ml. of acetonitrile. Anhydrous sodium carbonate (158 g., 1.5 mol.) was added to the solution and the mixture was refluxed for 23 hours. After cooling, the insoluble salt was filtered off and the filtrate was brought to dryness. The solid residue was recrystallized in about 20 ml. of hot ethanol. Melting point: 203-205°C. (B) 2,5,8,11-bicyclo[10,4,0]hexadec-14-ene Compound (A) (0.8 g., 3.17 mmol.) was added under nitrogen to 100 ml. of diglyme that had been dried and distilled over lithium aluminum hydride. The compound solubilized when the temperature was increased. Lithium aluminum hydride (3.91 g., 0.1 mol.) was added to the warm solution and the temperature was brought to 100°C. The reaction mixture was agitated for 15 days. After cooling, excess hydride was destroyed by the careful addition of ethyl acetate and water. The insoluble material was filtered off and washed with water and ethanol. The solvents were eliminated under vacuum at 80°C. The residue was dissolved in hydrochloric acid 6 M and the solution was brought to dryness on a rotary evaporator. The residue was added to about 20 ml. of ethanol. The suspension was refluxed for one hour and then kept overnight at 4°C. The precipitate was filtered and dried under vacuum. The free amine was obtained by adding the hydrochloride to trichloromethane and saturated with dry ammonia. The suspension was agitated overnight and the insoluble ammonium chloride was filtered off. The solvent was eliminated and a viscous liquid remained. Mass spectrum: (FAB, sample in 3-nitrobenzyl alcohol): m/e 225 (M+H) (C) 2,5,8,11-tetracarboxymethyl-2,5,8,11-tetraaza-bicyclo[10,4,0]hexadec-14-ene Bromoacetic acid (0.63 g.. 4.5 mol.) was dissolved in water and was slowly neutralized below 5°C with an aqueous solution of sodium hydroxide (0.18 g., 4.55 mmol., in 2 ml. of water). This solution was added to a solution of compound (B) (0.17 g., 0.76 mmol.) in 3 ml. of water. The reaction mixture was heated at 70 - 80° C. and a solution of sodium hydroxide (0.18 g., 4.55 mmol.. in 2 ml. of water) was added dropwise over 5 hours so as to maintain the pH between 9 and 10. The agitation was continued overnight at 80°C. The pH was lowered to 3 with concentrated hydrochloric acid and the reaction mixture was slowly concentrated under a stream of air. The title compound easily formed crystals and was recrystallized in a small volume of water. Yield: 45%, 0.15 g. 13 C NMR (in D 2 O, protonated form): 177.6, 177.0, 173.0, 172.8, 127.5, 124.7, 64.5, 58.4, 57.3, 57.0, 55.7, 53.6, 52.5, 52.4, 50.2, 48.8, 47.6, 26.0, 25.4 Mass spectrum (sample in 3-nitrobenzyl alcohol): m/e 479 (M + Na), 501 (M - H + 2 Na). Example 5 2,5,8,11-Tetracarboxymethyl-16(14)-bromo-14(16)-hydroxy-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane The dihydrochloride of the compound of Example 4 (0.2 g., 0.38 mmol.) was dissolved in 4 ml. of water and the pH was adjusted to 6. N-bromosuccinimide (0.07 g., 0.38 mmol.) was added to this solution at 10°C. The reaction mixture was stirred at 10°C for one hour until N-bromosuccinimide was totally dissolved. The clear solution was kept overnight at 4°C. The solvent was stripped off in a rotary evaporator and in the dark. The solid residue was dissolved in a minimum amount of methanol, and acetone was added until the solution became slightly cloudy. The compound was left to crystallize overnight at 4°C, and was collected by filtration in the dark. Yield: 45%. 13 C NMR (in D 2 O, protonated form): 179.2, 178.6, 173.1, 172.5, 71.6, 63.2, 62.8, 58.5, 58.4, 57.2, 56.7, 56.0, 53.7, 52.9, 52.6, 51.7, 50.2, 49.4, 48.0, 47.7, 29.2, 28.9, 27.8, 27.1 Mass spectrum: (sample in 3-nitrobenzyl alcohol): m/e 575 and 577 (M + Na), 597 and 599 (M - H + 2 Na). Example 6 Gadolinium (III) complexes of the ligands reported in Examples 1 - 5 All gadolinium (III) complexes were prepared by adjusting the pH of a stoichiometric mixture of gadolinium trichloride and one of the ligands reported in Examples 1 - 5 in water. The complexation reactions were started at about pH 3 and the solutions were heated to 60° C. for about 10 minutes before each addition of a few drops of a diluted aqueous solution of sodium hydroxide. The addition of sodium hydroxide was stopped when the pH reached 6.5. The complexation reactions were completed in a minimum of 3 to 4 hours. Each solution was concentrated and the products were purified by preparative HPLC. Evaporation of the appropriate fractions yielded colorless solids.

Claims: A compound of the formula: wherein R 1 and R 2 together with the carbon atoms in the tetraazacyclododecane macrocycle to which they attached form a fused fully or partially saturated non-aromatic six membered carbocyciic ring which may be unsubstituted or substituted by one or more halogen, straight or branched (C 1 -C 5 )alkyl, hydroxy or hydroxyalkyl groups, alkyl oxide of formula -R-OR wherein each R is a straight or branched (C 1 -C 5 )alkyl and which may be further fused to a phenyl or cyclohexyl ring, p is 0 or 1 and R 3 is hydrogen or straight or branched (C 1 -C 5 ) alkyl group; or a salt thereof A compound of claim 1 selected from the group consisting of 2,5,8,11-Tetracarboxymethyl-2,5,8,11-tetraaza-bicyclo[10,4,0]hexadecane; 2,5,11-Tricarboxymethyl-8,(2'-hydroxyethyl)-2,5,8,11-tetraazabicyclo [10,4,0]hexadecane; 2,5,8,11-Tetracarboxymethyl-14,15-benzo-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane; 2,5,8,11-Tetracarboxymethyl-2,5,8,11-tetraazabicyclo[10,4,0]hexadec-14-ene; and 2,5,8,11-Tetracarboxymethyl-16(14)-bromo-14(16)-hydroxy-2,5,8,11-tetraazabicyclo[10,4,0]hexadecane. A metal chelate comprising a compound of Claim 1 complexed with a metal atom. The chelate of Claim 3 wherein the metal is selected from atoms having an atomic number of 21 to 29, 42, 44, or 57 to 83. The chelate of Claim 4 wherein said metal is gadolinium. A pharmaceutical composition comprising a compound of any of the preceding claims and a pharmaceutically acceptable vehicle or diluent. A diagnostic pharmaceutical composition comprising at least a compound of claim 3, 4, or 5. Use of a compound of claims 3,4, or 5 for the preparation of a diagnostic formulation for obtaining images of organs and/or tissues of human or animal bodies, by the application of magnetic resonance imaging.

Inventors: DESREUX JEAN-FRANCOIS, MARINELLI EDMUND R, RATSEP PETER C, TWEEDLE MICHAEL F, WAGLER THOMAS R, DESREUX, JEAN-FRANCOIS, MARINELLI, EDMUND R., RATSEP, PETER C., TWEEDLE, MICHAEL F., WAGLER, THOMAS R.
Applicants: BRACCO INT BV, BRACCO INTERNATIONAL B.V.
Application year: 1994
Main classification: C07D257
IPC codes: C07D257