GLUCAGON ANALOGS WITH SERINE REPLACEMENTS AT POSITION 16 HAVING ANTAGONISTIC ACTIVITY

Abstract: Glucagon analogs characterized principally by the removal or replacement of the 9-aspartic acid residue or its replacement with another amino acid residue together with replacement of the 16-serine residue and possible replacement of the 11-serine residue and 21-aspartic acid residue with or without a histidine at the amino terminal are useful adjuncts to insulin therapy.

Description: GLUCAGON ANALOGS WITH SERINE REPLACEMENTS AT POSITION 16 HAVING ANTAGONISTIC ACTIVITY This invention was made with Government support under U. S . Public Health Grant DK24039 . The Government has certain rights in this invention . BACKGROUND OF THE INVENTION Glucagon is a 29-residue peptide hormone that regulates glycogenesis . The structure of glucagon may be represented as follows : His -Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys -Tyr-Leu- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Asp-Ser-Arg-Arg-Ala-Gin-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn- 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Thr 29 The abbreviations utilized herein are those recommended by IUPAC-IUB [See Eur. J. Biochem. 138, 9 (1984)] . The nomenclature used to define the peptides is that specified by Schroder & Lubke, "The Peptides", Academic Press (1965) , wherein in accordance with conventional representation the amino group at the N-terminal appears to the left and the carboxyl group at the C-terminal to the right . Where the amino acid residue has isomeric forms, it is the L- or naturally occurring form of the amino acid that is represented unless otherwise expressly indicated. Insulin, as is known, rapidly decreases elevated blood sugar. It is believed that, in humans, diabetes is only observed when insulin levels are low and glucagon levels are simultaneously elevated. The absence of insulin causes a rapid increase in blood glucose. Large amounts of insulin are required to reduce the glucose levels to normal. The maintenance of stable levels is difficult and subject to considerable fluctuation. This wide fluctuation is responsible, at least in part, for the clinical difficulties experienced in diabetes. Glucagon appears to act by binding to liver membrane receptors thereby activating adenylate cyclase which, in turn, triggers a series of reactions including the production of cyclic adenosine monophosphate (cAMP) , which activates phosphorylase and inhibits glucagon synthetase, thereby contributing to elevated glucose levels in the blood. Recently, considerable effort has been expended to develop glucagon antagonists that will bind to the liver membrane but do not have, the ability to transduce the signal to activate adenylate cyclase. One such product is Nα-trinitrophenol [12-homoarginine] glucagon. This product does bind to the glucagon receptor without significant activation of adenylate cyclase. It also activates another binding system in the hepatocyte membrane leading to the production of inositol triphosphate and calcium ions. A useful antagonist will block the action of endogenous glucagon by preventing it from binding to the liver membrane receptors and thereby producing cAMP and glucose in the cell, and the ultimate elevation of blood sugar. Such products would be useful to reduce a diabetic's need for injections or infusion of insulin. An ideal glucagon antagonist would (1) be completely inactive toward stimulation of adenylate cyclase and production of cAMP, (2) bind as well as glucagon itself to the liver membrane receptor, (3) compete with glucagon for receptor binding, (4) at moderate concentrations fully inhibit the action of glucagon toward the activation of adenylate cyclase, and (5) have a satisfactory inhibition index. The inhibition index is the molar ratio of antagonist to agonist which reduces the biological response 50% of the value in the absence of antagonist. It will be discussed more fully hereinafter. United States Patents 4,879,273 and 5,143,902 describe certain useful glucagon analogs in which the aspartic acid residue at the 9-position of the glucagon molecule is removed or replaced with another amino acid residue either in the D-form or the L-form. The replacement may be selected from any of a number of amino acids both natural and synthetic, including hydrophobic and hydrophilic amino acids, aliphatic amino acids, aryl amino acids, basic amino acids and acidic amino acids. It was observed that compounds of the class described in these patents either with or without the histidine " residue at the 1-position are useful adjuncts to insulin therapy in the control of blood glucose levels. The preferred compounds for such utility, as disclosed in the prior patents are: des His 1 [Gly 9 ] glucagon des His 1 [Nle 9 ] glucagon des His 1 [Lys 9 ] glucagon des His 1 [Glu 9 ] glucagon des His 1 [Glu 9 Lys 17 ' I8 Glu 21 ] glucagon and the corresponding carboxy terminal amides of such compounds . It has now been discovered that the utility of the compounds of the previous patents for the treatment of diabetes can be remarkably and surprisingly improved by replacement of the serine residue at the 16-position and the optional replacement of the serine residue at the 11- position and/or the aspartic acid residue at the 21- position. However, the key to the utility of the compounds is still the 9-position. THE INVENTION A novel class of glucagon antagonists has now been discovered which substantially fulfills the criteria listed above with surprising improvements and minimum side effects. The class is characterized by the deletion or replacement of the aspartic acid residue at the 9- position coupled with replacement of the serine residue at the 16-position and the optional replacement of amino acid residues at other positions. It has been observed that, generally, deletion of the histidine residue at the 1-position improves the desirable characteristics of the products of this invention. More specifically, the products of this invention, in addition to the position modifications outlined above, are characterized by a binding activity of at least about 40% relative to glucagon, an inhibition index up to about 10 and an adenylate cyclase activity up to about 1% glucaton. These remarkably improved therapeutic parameters have been made possible by the discovery of the significant contribution to binding arid transduction made possible by modifications in the glucagon molecule at positions other than the 1- and the 9-positions, especially by modifications of the 16-position. The replacement amino acid residues at the 16- position or, if desired, at the 11- and 21-positions are natural and synthetic aliphatic amino acids, preferably those containing up to five carbon atoms. The products of this invention were synthesized by known solid phase techniques. See, for example, Barany and Merrifield (1979) in The Peptides, eds. Gross and Meienhofer (Academic Press, New York) Vol. 2A, Pages 1- 284. The products can be prepared by manual methods or, for example, on a peptide synthesizer such as the Applied Biosystems 430 unit. Analogs with a free C-terminal carboxyl were made on phenylacetamidomethyl-resin supports, and those with C- terminal amides were made on a methylbenzhydrylamine- resin. Side chain protection was Arg(Tos) , Asp(OcHx) , Glu(OcHx) , His(Tos) , Lys (C1Z) , Ser(Bzl) , Thr(Bzl) , Trp(For) , and Tyr(BrZ) . Double couplings with preformed symmetric anhydrides in dimethylformamide were used routinely for all tert-butyloxycarbonyl-protected amino acids except for tosly arginine, glutamine, and asparagine, where N-hydroxybenzotriazole esters in dimethylformamide were required [Konig, W. & Gieger, R. Chem. Ber. 103, 788 (1970)] . The assembled protected peptide-resins were cleaved by the "low/high HF" technique [Tarn, J. P.; Heath, W. F. & Merrifield, R. B., J. Am. Chem. Soc. 105, 6442 (1983)] , which was developed to avoid a number of potential side reactions. After evaporation of HF and washing with ether, the crude free peptide was extracted with 10% acetic acid and lyophilized. Purification of the synthetic peptides was performed by preparative low- pressure reverse-phase liquid chromatography on C 18 -silica as described [Andreu, D. & Merrifield, R. B. in Peptides: Structure and Function, eds. Deber, C. M. ; Hruby, V. J. &• Kopple, K. D. (Pierce Chem. Co., Rockford, 111.) , pp. 595-598. The overall yields were between 35 and 40%. Homogeneity was demonstrated by analytical HPLC, and identity was confirmed by amino acid analysis, mass spectroscopy and molecular weight determinations. The amino acid analysis of all compounds prepared agreed with theory within +5%, and the molecular weights determined by mass spectrometry were within 0.5 mass units. Tert-Butyloxycarbonyl (Boc) protected amino acids were from Peninsula Laboratories, (San Carlos, (A.) p- methylbenzhydrylamine resin (0.45 mmol/g) was from United States Biochemical (Cleveland, Ohio) and boc-Thr- (Bzl) -4- oxymethylphenylacetamidomethyl copoly (styrene-1% divinyl benzene) was prepared as described by Mitchell et al., J. Org. Chem. 43, 2845 (1978) . 125 I-labeled glucagon from New England Nuclear was used without further purification for periods up to 1 month after its preparation. Creatine phosphate, creatine kinase, bovine serum albumin, dithiothreitol, GTP, and ATP were from Sigma. A cAMP assay kit containing [8- 3 H] cAMP was from Amersham. Nuflow membrane filters (0.45 urn) were from Oxoid (Basingstoke, England) . Various tests were employed to determine the efficacy of the products of this invention. These included the membrane receptor binding assay and adenyl cyclase assays. Membrane Binding Assay. Liver plasma membranes were prepared from male Sprague-Dawley rats (Charles River Breeding Laboratories) by the Neville procedure as described by Pohl [Pohl, S. L. (1976) in Methods in Receptor Research, ed. Blecher, M. (Marcel Dekker, New York) , pp. 160-164] . The receptor binding assay was as described by Wright and Rodbell [Wright, D. E. & Rodbell, M. (1979) J. Bio. Chem. 254, 268-269] in which competition for glucagon receptors between 125 I-labeled natural glucagon (1.6 nM) and the unlabeled synthetic analog was measured. After correction for the blank, the percentage of displacement of label was compared with that of a purified glucagon standard, and the relative binding affinity was calculated. Adenylate Cyclase Assay. The assay on liver membranes was performed according to Salomon et al. [Salomon, Y; Londos, C. & Rodgell, M. , Anal. Biochem. 58, 541, 548 (1974)] . The released cAMP was mixed with [8- 3 H] cAMP measured with a high affinity cAMP binding protein. The purpose of the membrane binding assay is to measure the ability of analogs of glucagon to bind to liver membrane receptor compared to that of glucagon. When the glucagon analogs of this invention were assayed, they were assayed as amides with natural glucagon amide as a standard, thus eliminating the possibility of imprecision due to the heterogeneity of membrane preparations. In fact, it presently appears that C-terminal amides are more active than the corresponding carboxyl compounds. Accordingly, C- terminal amides of the glucagon analogs of the invention are the presently preferred compounds of the invention. The relative binding affinity of a given analog is expressed as: (half maximal displacement concentration of glucacron) x ι 00 (half maximal displacement concentration of analog) The purpose of the adenylate cyclase assay is to measure the ability of the compound under test to stimulate the activity of adenylate cyclase. The assays are used to measure relative potency, maximum activity and inhibition index. The inhibition index, defined above, was determined from adenylate cyclase assays by two different protocols . 1. A glucagon standard curve for cAMP vs. glucagon concentration was established. Then another glucagon assay curve was measured in the presence of a constant amount of antagonist . The concentration of glucagon that had its activity reduced to 50% by that concentration of inhibitor was then determined. 2. A series of tubes were set up containing an amount of glucagon which will produce 90% of maximum response. Increasing amounts of antagonist were then added and the concentration that reduced the response to 45% of maximum was determined. The compounds of this invention have an inhibition index less than 10, membrane receptor binding activity of at least 40% compared to glucagon and an adenylate cyclase activity up to about 1% of glucagon. In the most preferred compounds of this invention, the pA 2 value is at least 5 and preferably above 7. The pA 2 value is the negative logarithm of the concentration of antagonist that reduces the response to 1 unit of agonist to the response obtained from 0.5 unit of agonist . The following table shows the results of measurements with glucagon and certain of the presently preferred compounds of this invention measured as amides . TABLE 1 Analog of % Binding % Relative (I/A) 50 P 2 Glucagon Amide Activity Activity Glucagon Amide 100 15 1. NkΛy^Gln 11 46.8 0.003 1.6 8.7 2. des-His le^la 16 100 0.001 2.14 8.4 3. des-His 1 Nle 9 Ala 11 Ala 16 100 0.0043 0.85 8.4 4. des-His'Nle'Ala'^In 16 56.3 0.0011 2.2 8.4 5. des-Hi^GhAUa 1 ■ Ala 16 Glu 21 39 0.016 2.57 8.5 The glucagon analogs of this invention also include derivatives having the defined properties. As indicated above, C-terminal amides are actually preferred over the C-terminal carboxyl compounds. Side chain amides such as amides of dibasic acids are also useful. Esters, especially those based on alkyl or aralkyl alcohols corresponding to the amides, may also be employed. Ethers, especially lower alkyl ethers of analogs including Ser, Thr and Tyr amino acid residues are also useful, as are esters of these analogs based on alkyl, aryl and aralkyl acids . Glucagon analogs containing amino acid residues with additional functional group may also be converted to derivatives within the scope of the invention. These might include, for example, N-acetyl derivatives of diamino acids such as lysine. One class of useful derivatives is based on des-His 1 glucagon analogs in which the amino group of the amino terminal serine residue has been converted to a 2,4- diflurobenzoyl amide. Other hydroxyl and amine substituted amino acids derivatized with 2,4- difluorobenzoic acid are within the scope of the invention whether or not the histadyl residue at the 1- position is in place. In some instances, compounds within the scope of the invention may be synthesized and thereafter utilized with one or more of the blocking groups still in place. The products of this invention will generally be administered in the same manner as insulin, i.e., parenterally or by infusion. Since their chemical structure and activity is quite similar to insulin, they will generally be administered with the same types of pharmaceutically acceptable excipients as insulin. They may in fact be coadministered with insulin in the same dosage units . They may also be administered simultaneously with the insulin although not in the same composition. Since the products of the invention are amphoteric, they may be utilized as free bases, as acid addition salts or as metal salts. The salts must, of course, be pharmaceutically acceptable, and these will include metal salts particularly alkali and alkaline earth metal salts, suitably potassium or sodium salts . A wide variety of pharmaceutically acceptable acid addition salts are available. These include those prepared from both organic and inorganic acids, preferably mineral acids. Typical acids, which may be mentioned by way of example, include citric, succinic, lactic, hydrochloric and hydrobromic acids. Such products are readily prepared by procedures well known to those skilled in the art. The products of the invention will normally be provided for as parenteral compositions for injection or infusion. They can, for example, be suspended in an inert oil, suitably a vegetable oil such as sesame, peanut, or olive oil. Alternatively, they can be suspended in an aqueous isotonic buffer solution at a pH of about 5.6 to 7.4. Useful buffers include sodium citrate-citric acid and sodium phosphate-phosphoric acid. The desired isotonicity may be accomplished using sodium chloride or other pharmaceutically acceptable agents such as dextrose, boric acid, sodium tartrate, propylene glycol or other inorganic or organic solutes . Sodium chloride is preferred particularly when the buffer contains sodium ions. If desired, the solutions may be thickened with a thickening agent such as methyl cellulose. They may be prepared in emulsified form, either water in oil or oil in water. Any of a wide variety of pharmaceutically acceptable emulsifying agents may be employed including, for example, acacia powder, or an alkaryl polyether alcohol sulfate or sulfonate such as Triton. The therapeutically useful compositions of the invention are prepared by mixing the ingredients following generally accepted procedures. For example, the selected components may be simply mixed in a blender or other standard device to produce a concentrated mixture which may then be adjusted to the final concentration and viscosity by the addition of water or thickening agent and possibly a buffer to control pH or an additional solute to control tonicity. For use by the physician, the compositions will be provided in dosage unit form containing an amount of glucagon analog which will be effective in one or multiple doses to control glucoenesis and ketogenesis at the selected level, normally in the presence of insulin. As will be recognized by those skilled in the art, and effective amount of the therapeutic agent will vary with many factors including the age and weight of the patient, the amount of insulin which is concurrently employed, the blood sugar level to be obtained, the inhibition index of the selected analog, and other factors. Typical dosage units will contain from 0.2 to 0.8 ug/ml although wide SUBSTITUTE SHEET (RUtf 26)- variations from this range are possible while yet achieving useful results .

Claims: WHAT IS CLAIMED IS: 1. Glucagon analogs characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-position with an aliphatic amino acid residue, said analog being further characterized by a relative membrane receptor binding activity compared to glucagon of at least about 40%, an inhibition index up to about 10% and an adenylate cyclase activity up to about 1% of that of glucagon and pharmaceutically acceptable acid addition salts of said analogs . 2. Glucagon analogs of claim 1 further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid. 3. A des-His 1 glucagon analog of claim 1 or a pharmaceutically acceptable acid addition salt thereof. 4. A des-His 1 glucagon analog of claim 2 or a pharmaceutically acceptable acid addition salt thereof. 5. An amide of a glucagon analog of claim 1. 6. An amide of a glucagon analog of claim 2. 7. Nle 9 Ala n Gln 16 glucagon amide. 8. des-His^le^la 16 glucagon amide. 9. des-His 1 Nle 9 Ala 11 Ala 16 glucagon amide. 10. des-His'Nle^la'^ln 16 glucagon amide. 11. des-His 1 Glu 9 Ala 11 Ala 16 Glu 21 glucagon amide. 12. A parenteral composition for control of glycogenesis and ketoacidosis in humans containing a pharmaceutically acceptable carrier and an amount which is effective to achieve such control of a glucagon analog which is characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-position with an aliphatic amino acid residue, said analog being further characterized by a relative membrane receptor binding activity of at least about 405 compared to glucagon, an inhibition index up to about 10% and an adenylate cyclase activity up to about 1% of that of glucagon, and pharmaceutically acceptable acid addition salts of said analogs. 13. A parenteral composition of claim 12 wherein the glucagon analog is further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid. 14. A parenteral composition of claim 12 wherein the glucagon analog is a des-His 1 analog further characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-position with an aliphatic amino acid residue, said analog being further characterized by a relative membrane receptor binding activity of at least about 40% compared to glucagon an inhibition index up to about 10% and an adenylate cyclase activity up to about 1% that of glucagon, and pharmaceutically acceptable acid addition salts of said analogs. 1 15. A parenteral composition of claim 13 wherein 2 the glucagon analog is a des-His 1 analog further 3 characterized by replacement of the serine residue at the 4 ll-position, the aspartic acid residue at the 21-position 5 or both residues with an aliphatic amino acid. 1 16. A parenteral composition of claim 12 wherein 2 the glucagon analog is an amide characterized by (1) 3 removal of the aspartic acid residue at the 9-position or 4 its replacement with another amino acid residue and (2) 5 the replacement of the serine residue at the 16-position 6 with an aliphatic amino acid residue, said analog being 7 further characterized by a relative membrane receptor 8 binding activity of at least about 40% compared to 9 glucagon, an inhibition index up to about 10% and an 10 adenylate cyclase activity up to about 1% that of .11 glucagon, and pharmaceutically acceptable acid addition • 12 salts of said analogs. 1 17. A parenteral composition of claim 12 wherein 2 the amide is further characterized by replacement of the 3 serine residue at the ll-position, the aspartic acid 4 residue at the 21-position or both residues with an 5 aliphatic amino acid. 1 18. A parenteral composition of claim 12 wherein 2 the glucagon analog is a des-His 1 amide characterized by 3 (1) removal of the aspartic acid residue at the 9- 4 position or its replacement with another amino acid 5 residue and. (2) the replacement of the serine residue at 6 the 16-position with an aliphatic amino acid residue, 7 said analog being further characterized by a relative 8 membrane receptor binding activity of at least about 40% 9 compared to glucagon, an inhibition index up to about 10% 10 and an adenylate cyclase activity up to about 1% that of 11 glucagon pharmaceutically acceptable acid addition salts 12 of .said analogs. 19. A parenteral composition of Claim 18 wherein the glucagon analog is a des-His 1 amide further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid. 20. A parenteral composition of claim 12 wherein the glucagon analog is Nle Q Ala π Gln 16 glucagon amide. 21. A parenteral composition of claim 12 wherein the glucagon analog is des-His^le^la 16 glucagon amide. 22. A parenteral composition of claim 12 wherein the glucagon analog is des-His 1 Nle 9 Ala 11 Ala 16 glucagon amide. 23. A parenteral composition of claim 12 wherein the glucagon analog is des-His 1 Nle 9 Ala 11 Gln 16 glucagon amide. 24 . A parenteral composition of claim 12 wherein the glucagon analog is des-His 1 Glu 9 Ala 11 Ala 16 Glu 21 glucagon amide . 25. A parenteral composition in dosage unit form for the control of glycogenesis and ketoacidosis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-position with an aliphatic amino acid residue, said analog being further characterized by a relative membrane receptor binding activity of at least about 40% compared to glucagon, an inhibition index up to about 10% and an adenylate cyclase activity up to about 1% that of glucagon, and pharmaceutically acceptable acid addition salts of said analogs. 26. A parenteral composition of claim 25 in dosage unit form for the control of glucogenesis and ketoacidosis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid. 27. A parenteral composition of claim 25 in dosage unit form for the control of glucogenesis and ketoacidosis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is a des-His 1 analog characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-positio-n with an aliphatic amino acid residue, said analog being further characterized by a relative membrane receptor binding activity of at least about 40% compared to glucagon, an inhibition index up to about 10% and an adenylate cyclase activity up to about 1% that of glucagon, and pharmaceutically acceptable acid addition salts of said analogs. 28. A parenteral composition of claim 27 in dosage unit form for the control of glucogenesis and ketoacidosis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is a des-His 1 analog further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid . SUBSTITUTE SHEET {RULE ' 26) 29. A parenteral composition of claim 25 in dosage unit form for the control of glycogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is an amide characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-position with an aliphatic amino acid residue, said analog being further characterized by a relative binding activity of at least about 40% and an inhibition index up to about 10% and an adenylate cyclase activity up to about 0.005 or pharmaceutically acceptable acid addition salts of said analogs. 30. A parenteral composition of claim 25 in dosage unit form for the control of glycogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is an amide further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid. 31. A parenteral composition of claim 25 in dosage unit form for the control of glycogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is a des-His 1 amide characterized by (1) removal of the aspartic acid residue at the 9-position or its replacement with another amino acid residue and (2) the replacement of the serine residue at the 16-position with an aliphatic amino acid residue, said analog being further characterized by a relative binding activity of at least about 40% compared to glucagon, an inhibition index up to about 10% and an adenylate cyclase activity SUBSTITUTE SHEET (RULE ft) up to about 1% that of glucagon, and pharmaceutically acceptable acid addition salts of said analogs. 32. A parenteral composition of claim 30 wherein the glucagon analog is des-His 1 amide further characterized by replacement of the serine residue at the ll-position, the aspartic acid residue at the 21-position or both residues with an aliphatic amino acid. 33. A parenteral composition of claim 25 in dosage unit form for the control of glucogenesis and ketoacidosis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is Nle 9 Ala n Gln 16 glucagon amide. 34. A parenteral composition of claim 25 in dosage unit form for the control of glycogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is des-His^Nle^la 16 glucagon amide. 35. A parenteral composition of claim 25 in dosage unit form for the control of glycogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is des-His 1 -Nle 9 Ala 11 Ala 16 glucagon amide. 36. A parenteral composition of claim 25 in dosage unit form for the control of glycogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is des-His 1 -Nle 9 Ala 11 Gln 16 glucagon amide. 37. A parenteral composition of claim 25 in dosage unit form for the control of glucogenesis in humans containing a pharmaceutically acceptable carrier and from about 0.2 to 0.8 ug/ml of a glucagon analog wherein the glucagon analog is des-His 1 -Glu 9 Ala 11 Ala 16 Glu 21 .

Inventors: MEERIFIELD ROBERT B, UNSON CECILIA G, MEERIFIELD, ROBERT, B., UNSON, CECILIA, G.
Applicants: UNIV ROCKEFELLER, THE ROCKEFELLER UNIVERSITY
Application year: 1994
Main classification: C07K14
IPC codes: A61K38, A61P3, C07K14