NEW NIMESULIDE SALT CYCLODEXTRIN INCLUSION COMPLEXES

Abstract: Inclusion complexes of nimesulide alkali and alkaline earth salts of general formula (I) where - A stands for an alkali and alkaline earth ion - with cyclodextrins and cyclodextrin derivatives, compositions containing the same, processes for the preparation of the complexes by complexation of nimesulide salts and the compositions as well as methods to use the same as pharmaceuticals.

Description: New Nimesulide Salt Cyclodextrin Inclusion Complexes The invention relates to highly soluble, physiologically accep¬ table inclusion complexes of nimesulide- salts with cyclodex- trins, to the preparation thereof, to pharmaceutical compo - sitions containing the same as well as methods for their use. More particularly the invention relates to inclusion complexes of nimesulide alkali and alkaline earth salts of general formula (I) with cyclodextrins and cyclodextrin derivatives. In this specification in the general formula -A always stands for an alkali and alkaline earth ion. Nimesulide [4*-nitro-2-phenoxy-methane-sulfonanilide] is known to be a potent non-εteroidal antiinflammatory drug successfully used for the treatment of different painful inflammatory condi¬ tions, rheumatoid arthritis and it possesses antipyretic acti¬ vities too (Belgian Patent N" 801812). Solutions of nimesulide sodium salts were prepared from nimesulide with sodium carbonate in acetone and they were used without isolation as intermedia¬ tes to prepare N-substituted nimesulide derivatives (Belgian Pa¬ tent N β 801812). Probably due to the high pH value of their so¬ lutions the nimesulide alkali and alkaline earth salts were not used practically as pharmaceuticals. Recently it has been con¬ firmed that based on its mechanism of action in pain relief, ni¬ mesulide can be also considered to represent a new type of use¬ ful analgesic agents. In case of such drugs a quick onset of ac¬ tion of the orally administered formulation is a very important factor. Compared to other non-steroidal antiinflammatories, nimesulide has a favorable therapeutic index, minimal acute gastrointe¬ stinal toxicity and shows good general tolerability. It is che¬ mically different from other drugs of its class, because its functional acidic group is a sulfonanilide moiety. Nimesulide is a very hydrophobic drug substance practically in¬ soluble in water, its aqueous solublity is about 0.01 mg/ml at room temperature. The very poor aqueous solubility and wettab- ility of the drug present problems for the preparation of phar¬ maceutical formulations with good release and non-variable bio- availability. To overcome the disadvantages connected with the very poor aqueous solubility and wettability the increase of aqueous solu¬ bility is an essential aim. Nimesulide is a weak acid type compound therefore its aqueous solubility in acidic medium, e.g. at the pH of the gastric juice is particularly poor. Orally administered nimesulide is likely to be absorbed only in the lower part of the gastrointestinal tract, probably this explains the rather protracted onset of its biological effect. Complexation of nimesulide with cyclodextrins is described pre¬ ferably with β-cyclodextrin in 1:1 molar ratio whereby faster absorption and higher plasma levels of nimesulide are shown in animal tests as compared with administration of nimesulide per se (Patent Applications PCT/IT91/00043 and DE 4116659). For solid complex preparation three different known methods are exemplified: a. precipitation from water and organic solvent mixture by sha¬ king overnight, the preferred solvent being methylene-chloride, b. freeze- or spray- drying from homogeneous aqueous ammonium hydroxide solution. c. stirring in aqueous suspension for several days at 60 * C and isolating the complex by evaporation under reduced pressure. Method a. is not acceptable for preparation of CD complexes for pharmaceutical purposes. All organic solvents form more or less stable complexes with cyclodextrins. Inclusion of methylene chloride by β-cyclodextrin is inevitable in this case, conse¬ quently the product might contain a considerable amount of toxic chlorinated solvent. This can not be removed completely even by heating in vacuo at elevated temperature for hours, it will be released only upon dissolution e.g. in the gastric juice. Method c. is the oldest known method for preparation of drug/cyclodex- trin-complexes, but the long stirring time, with the concomitant degradation makes this process technically obsolete. Method b. seems to be the best, however it is difficult to completely re¬ move ammonia during the freeze-drying procedure. In the said Patent Application no data are given about the at¬ tainable solubility enhancement of nimesulide with BCD or the dissolution behaviour of the complexes prepared by the three different methods described in the Patent Application. It can be concluded that both pH alteration towards the alkaline region and complexation with BCD can enhance the solubility of nimesulide. BCD alone shows only a very moderate (about 5-fold) solubility enhancing effect which means 0.05 - 0.06 mg/ l dis¬ solved nimesulide in a saturated aqueous BCD solution. However, significantly higher increase in solubility can be achieved on¬ ly at pH beyond the physiologically acceptable values. The object of the present invention was to prepare highly solu¬ ble, physiologically acceptable inclusion complexes comprising nimesulide and cyclodextrins. Another object of the invention was to provide efficient method(ε) for producing said complexes having the said solubility or redissolving properties. One object of the present invention are the new inclusion com¬ plexes of nimesulide alkali and alkaline earth salt of general formula (I) with cyclodextrins and cyclodextrin derivatives. These new products ensure a considerable 200-600 fold increase in solubility of nimesulide at physiological pH due to the syn- ergetic effect of pH alteration and cyclodextrin complexation. Preferred embodiments of the invention are inclusion complexes wherein the cyclodextrins and cyclodextrin derivatives are α, B and gamma cyclodextrins, and alkyl or hydroxyalkyl derivatives of cyclodextrin, preferably methyl B-cyclodextrins or hydroxy- propyl-B-cyclodextrin. Further products of preference are inclusion complexes wherein the metal ion in the nimesulide salt is sodium of potassium. Alkaline earth salts e.g. the calcium or magnesium salts might also be used. A further embodiment of the invention is the process for the preparation of inclusion complexes of nimesulide alkali and al¬ kaline earth salts and cyclodextrins or cyclodextrin derivati¬ ves reacting nimesulide alkali and alkaline earth salts in the presence of water with cyclodextrins or cyclodextrin derivatives at pH 7 to 9.5 preferably at pH 7.5 to 8.5. When carrying out this process it is advantageous to use nime¬ sulide alkali or alkaline earth salts formed in situ in the re¬ action mixture by adjusting the suspension of nimesulide in wa¬ ter to a pH value of 7 to 9.5 preferably 7.5 to 8.5 by addition of alkali and alkaline earth hydroxides, alkali and alkaline earth carbonates, alkali and alkaline earth hydrogen carbona¬ tes, alkali and alkaline earth phosphates, preferably sodium hydroxide, disodium phosphate and/or sodium hydrogen carbonate. Buffers may be used to adjust the desired pH-values. After formation of the complex water may be removed by freeze- drying, spray-drying, low temperature vacuum evaporation, vacu¬ um drying or other known methods. Aqueous solutions of the com¬ plexes or solutions containing the complex formed in situ from the ingredients nimesulide salt and cyclodextrins or cyclodex¬ trin derivatives are also objects of the present invention. The inclusion complexes according to the present invention were prepared with α, B and gamma-cyclodextrin or with highly soluble hydroxy alkylated and methylated β-cyclodextrin derivatives preferably with randomly methylated β-cyclodextrin, DIMEB or TRIMEB. The inclusion complexes according to the invention can easily be redissolved in distilled water or physiological saline to obtain clear or slightly opalescent solutions at physiological pH va¬ lues of 200-600 times higher dissolved nimesulide concentration than its aqueous solubility. Further objects of the invention are new pharmaceutical compos¬ itions containing as active ingredient the highly soluble, phy¬ siologically acceptable inclusion complex of nimesulide alkali and alkaline earth salt and cyclodextrins or cyclodextrin deri¬ vatives as stated above. Pharmaceutical compositions of particular importance are those containing as active ingredient the inclusion complex of nimesu¬ lide sodium salt and B cyclodextrin. The compositions may con¬ tain other pharmaceutically acceptable ingredients such as used for formulation by the pharmaceutical industry. The complexes and compositions according to the present in¬ vention can be used in pharmaceutical formulations administered by oral, parenteral, rectal or topical route. The aqueous solu¬ tions of the complexes can also be used in sprays. A further embodiment of the invention consists in methods of treating patients in need of antiinflammatory and/or analgetic treatment administering to the patient an effective amount of an inclusion complex of cyclodextrins or cyclodextrin derivatives formed with nimesulide alkali and alkaline earth salt. Most probably the complex - after dissolution in the gastro¬ intestinal tract - is subject to an equilibrium whereby mole- cularly dispersed nimesulide is formed in the gastric juices , accelerating and improving absorbance and action of the drug. The invention is illustrated by the following Examples without restricting the scope to their contents. Examples on chemical synthesis and solubility Example 1. Excess amounts of nimesulide were stirred at 30°C in 5 ml samples of distilled water, pH 7.6, 8.0 and 9.6 alkali phospha¬ te buffer solutions containing 0.0, 0.5, 1 and 1.8% (w/v) of B- cyσlodextrin. After 18 hours of equilibration the suspensions were filtered across a 0.45 μm membrane filter. - The dissolved nimesulide contents of the filtrates were analyzed by spectrop- hotometry after appropriate dilution with 0.05 N hydrochloric acid in 50% (v/v) ethanol. Absorbance at - max 300±3 nm was used for quantitative calculation. Table l. summarizes the obtained results, whereby final pH values of the filtered solutions are also indicated. TABLE 1 BCD Dissolved Nimesulide Final pH of solutions mg/ml % dist. pH 7.6 pH 8.0 pH 9 pH 7.6 pH 8.0 pH 9.6 water 0 0.010 0.034 0.07 0.28 7.40 7.70 8.30 0.5 0.024 0.170 0.26 0.80 1.0 0.035 0.330 0.42 1.27 7.30 7.46 8.09 1.8 0.054 0.570 0.85 1.79 7.24 7.45 7.92 Nimesulide-Na solutions alone and in the presence of equimolar BCD were titrated with 0.1N H 3 PO 4 . At different pH values the opalescence of the solution became stronger (drug precipitation) . Samples are analysed for dissolved nimesulide content by UV spectrophotometry. Figure 2 shows the decrease of nimesulide solubility in the presence and absence of BCD as a function of pH. Dissolved nimesulide in mg/ml is shown against pH values. At around pH 8 almost all nimesulide remains dissol¬ ved in presence of BCD while almost the whole drug precipitates from the control solution. (About 10 mg/ml dissolved nimesulide as compared with less than 1 mg/ml). pK a of nimesulide is shif¬ ted to lower value by CD- complexation. Example 2. 95 g B-cyclodextrin (0.076 moles, water content 10%) are suspended in 1200 ml of distilled water with vigorous stirring and 12 g (0.038 moles) of nimesulide dissolved in 80 ml of 0.5 N aqueous sodium hydroxide solution are added. When obtaining a homogeneous solution the pH of the solution is adjusted with 0.5 M H 3 PO4 to pH 8.2-8.6 and the yellow solution is feeze-dried to isolate the solid complex. 98 g nimesulide sodium salt/BCD com¬ plex of 1:2 molar ratio (a bright yellow fine powder) are obtained. Nimesulide content: 11.8±0.1% measured by UV- spec¬ trophotometry. Solubility properties of the complex: 100 mg of the product can be dissolved in 3 ml of distilled water resulting in a yellow solution with approximately 4 mg/ml nimesulide content, the so¬ lution having a pH value of 7.6±0.1. DSC curve of the complex is identical with that of the complex obtained according to Example 3 below. Disappearance of the en- dothermic peak at 240 - 241 °C points to the absence of free ni¬ mesulide in the inclusion complex of 1:1 nimesulide sodium-BCD (molar ratio) . Example 3. 33.2 g of β-cyclodextrin (0.025 moles, water content 13.7%) are suspended in 550 ml of distilled water. 8.25 g of nimesulide (0.025 moles) are dissolved in 60 ml of a 0.5 N aqueous sodium hydroxide solution and added to the suspension of β-cyclodextrin under vigorous stirring resulting in a clear dark yellow solution. The pH of the solution is adjusted with 0.5 N H3PO4 to pH 8.5-8.7 and the solution is freeze-dried to obtain the so¬ lid complex. 41 g of nimesulide-sodium salt/BCD complex of 1:1 molar ratio are obtained as a yellow fine powder. Nimesulide content: 20.0±0.2% measured by UV-spectrophotometry. Solubility properties: 100 mg of the complex can be dissolved in 6 ml of distilled wa¬ ter resulting in a slightly opalescent solution with approxi¬ mately 3.5 mg/ml nimesulide content, the solution having a pH value of 8.3±0.1. Differential scanning calorimetry (DSC) curves show character¬ istic differences between the physical mixture and the lyophili- zed complex. The sharp endother ic heat flow peak characteristic for the melting of nimesulide appears at 240 - 241 β C on the DSC curve of the physical mixture, followed by a strong exothermic DSC peak characteristic for thermal decomposition of BCD . The DSC pattern of the inclusion complex; does not show any endother¬ mic heat flow in the melting range indicating the formation of an inclusion complex between the salt and β-cyclodextrin, only a strong exothermic DSC peak characteristic for thermal decomposi¬ tion of BCD can be observed. Figure 3 shows the DSC curves of the nimesulide-sodium : BCD = 1:2 physical mixture (A) and the nimesulide-sodium/BCD complex (B) prepared according to Example 3 : Heat flow (mW) is repre¬ sented as a function of temperature ( e C) [Du Pont 1090 Thermal Analyzer, scanning rate 5 e C/min, argone atmosphere]. The nimesulide-potassium salt/BCD complex is prepared according to the same method, using KOH instead of NaOH. Similarly the nimesulide-calcium and magnesium salt/CD complexes can also be prepared. Example 4. 30.2 g of β-cyclodextrin (0.024 moles, water content 10%) and 3.75 g of nimesulide (0.012 moles) are suspended in 25 ml of a 0.5 N aqueous sodium hydroxide solution. The thin suspension is stirred by Ultra Turrax high speed dispersing apparatus with r.p.m. approx. 103 for five minutes. The pH of the alkaline non-transparent solution is adjusted below pH 9. with 1 N aqueous hydrochloric acid. The solid complex is isolated by dry- ing at 40°C under vacuo, and the dry complex in powdered. 34 g of nimesulide-sodium salt-BCD co lex (1:2) are obtained as a yellow fine powder. Nimesulide content: 11±0.1% measured by UV-spectrophotometry. Solubility : 100 mg of the complex dissolved in 3 ml of distil¬ led water resulted in an opalescent solution with approximately 3 mg/ml dissolved nimesulide content, the solution showing a pH value of 7.3±0.1. Example 5. 40 g of randomly methylated β-cyclodextrin (RAMEB 0.034 moles, average degree of substitution per glucose unit is 1.8) are dis¬ solved in 300 ml of distilled water. 5.4g of nimesulide (0.017 moles) dissolved in 17 ml of 1 N aqueous sodium hydroxide are added whereupon the pH of the solution is adjusted with 0,5 N H 3 PO4 to pH 7.7±0.1. The yellow solution is freeze-dried to iso¬ late the solid complex. 45 g of nimesulide-sodium salt-randomly methylated-β- cyclodex¬ trin (RAMEB) complex of 1:2 molar ratio are obtained in the form of a fine yellow powder. Nimesulide content: 11.1±0.1% measured by UV-photo etry. Solubility: 100 mg of the complex dissolved in 2 ml of distil¬ led water result in a yellow solution (pH 7.3±0.1) with appro¬ ximately 6 mg/ml nimesulide content. Example 6. 2.6 g of gamma-CD (0.002 moles) are dissolved in 20 ml of dist¬ illed water and 0.308 g (0.001 moles) of nimesulide dissolved in 5 ml of 0.2 N aqueous sodium hydroxide are added whereupon the pH of the solution is adjusted to pH 7.4-7.5, and the yellow solution is freeze-dried. 2.9 g of nimesulide sodium salt/gam¬ ma-CD complex of 1:2 molar ratio are isolated in the form of a very fine yellow powder. Nimesulide content: 10.5 ± 0.2% measu¬ red by UV-photometry. 100 mg in 2 ml of distilled water give a clear yellow solution with approximately 5 mg/ml nimesulide content (pH= 7.3 ± 0.1). Example 7. 13 g of hydroxypropylated BCD (0.01 mole average degree of sub- stitutaion per glucose unit is 2.7) are dissolved in 150 ml of distilled water and 1.54 g of nimesulide (0.005 M) and 5 ml of IN sodium hydroxide are added while stirring. A dark clear yel ¬ low solution is obtained the pH of which is adjusted to 7.5 ± 0.1 with 0.2 N phosphoric acid, and the solution is freeze- dried. 14 g of nimesulide sodium/HPBCD complex of 1:2 molar ratio are obtained in the form of a very fine yellow powder. Nimesulide content: 10.6 ± 0.2% measured by UV photometry. 100 mg of this complex dissolved in 2 ml of distilled water re¬ sulting in a clear yellow solution with about 5 mg/ml dissolved nimsulide content (pH of the solution = 7.4 ± 0.1). Example 8. The dissolution of nimesulide-Na/BCD complex prepared according to Example 2 was compared to i esulide-Na and nimesulide- Na/BCD complex prepared in situ from the corresponding 1:2 molar physical mixture of the components. Simulated gastric juice was used as a medium. 100 mg of nimesulide, an equivalent amount of the isolated complex and the physical mixture of the ingredients were stirred in 20 ml of pH 1.4 aqueous HC1 solution. Samples were taken at 2, 15 and 60 minutes. On filtration the nimesulide content was measured by UV photometry. Average results of three experiments are summarized in Table 2. Table 2. Concentration of Dissolved Nimesulide (μg/ml) time ( in) Nim.-Na Nim.-Na/BCD Nim.-Na/BCD isolated complex in situ complex 2 5.4 ± 0.7 35.4 ± 1.4 33.3 ± 2.2 15 4.0 ± 0.1 36.1 ± 0.25 33.3 ± 0.3 60 4.4 ± 0.5 34.6 ± 1.5 35.1 ± 0.2 The measurable nimesulide concentration both for the isolated complex and the in situ formed complex are approximately five times higher than in the case of nimesulide-Na substance. This higher concentration is maintained even after 60 minutes. The results indicate that in situ complex formation from the physi¬ cal mixture took place under the conditions employed. Example 9. A comparative solubility test was carried out using nimesulide- Na/BCD complex tablets (100 mg) , Mesulid commercial tablets (batch N° 891 1026/SCAD 91/11. 100 mg) and nimesulide-Na salt substance (prepared by lyophilization from a solution of 1:1 mo¬ lar ratio nimesulide and sodium hydroxide, using an equivalent amount to 100 mg of ni sulide) . Powdered tablets of each sample were suspended in 20 ml of pH 1.4 aqueous HC1 solutions and stirred at ambient temperature. Samples were taken after 2, 60 and 90 minutes. On filtration the nimesulide concentration of the filtrates was evaluated by UV- spectrophoto etry after dilution with 96% ethanol. Absorbance atΛ max = 299 ± 1 nm was used for quantitative cal- culation taking Eicm 299 ± 1 nm = 257 for nimesulide. Results are summarized in Table 3. Table 3. Nimesulide concentrations as a function of time at pH = 1.4 Nimesulide cone, (μg/ml) 2 min. 60 min. 90 in. Nim.-Na/BCD tbl. 35 43 37 Mesulid tbl. 10 14 11 Nim.-Na salt 7 10 6 Table 3. shows that considerable solubility differences are found in favour of the complex tablets. It is obvious that the solubility of an acid type drug might be lower in a pH 1.4 solu¬ tion than in water. The alkali salts of the drug are freely so¬ luble in water. Their in vivo absorption however after oral ad¬ ministration is delayed owing to the precipitation of the acid- form under the pH of the stomach. BCD complexation enhances solubility of nimesulide also under acidic pH conditions. Based on the above in vitro findings an improved absorption of nimesulide-salt complexes is understood after oral administration because the solubility under acidic pH is a necessary precondition e.g. for faster onset of action. Example 10. 2.3 g of BCD (0.002 moles, water content 14%) and 0.31 g of ni¬ mesulide (0.001 mole) are suspended in 100 ml of distilled wa¬ ter. 2 ml of 0.5N aqueous potassium hydroxide are added while stirring. pH of the dark yellow solution obtained is adjusted below 9 using 0.5N hydrochloric acid. 2.8g of nimesulide-K/BCD complex ( 1 :2) are isolated by freeze-drying. Nimesulide content: 10.8 ± 0.2% (UV spectr.) Solubility: 100 mg of the above complex are dissolved in 3 ml of distilled water. A clear or slightly opalescent solution with « 3mg/ml dissolved nimesulide results, pH 7.8 ± 0.1. Examples on Pharmaceutical Compositions. Example 11. Composition of tablets with 50 mg and 100 mg of nimesulide con¬ tent: nimesulide-sodium salt/BCD complex ( Example 3) 250 mg 500 mg calcium phosphate 60 mg 85 mg lactose 35 mg 45 mg magnesium-stearate 5 mg 5 mg Total 350 mg 540 mg The complex is homogenized with the additives and directly pres¬ sed into tablets. Example 12. Composition of granule sachet formulation with 50 mg and 100 mg of nimesulide content of each. nimesulide-sodium salt/BCD 450 mg 900 mg sorbite 2500 mg 4000 mg lemon flavour 15 mg 30 mg saccharine 5 mg 5 mg Total 2970 mg 4935 mg The complex is homogenized with sorbite and additives and filled into sachets. Example 13. Composition of oral liquid formulation with 50 mg/10 ml nimesu¬ lide content nimesulide-K/BCD complex (Ex 3.) 2.500 g hydroxypropyl cellulose 0.200 g potassium sorbate 0.150 g fructose 5.0 g saccharine sodium qu.sat. demineralized water ad 100.0 ml The viscosity enhancer is dissolved in about 80 ml of warm demi¬ neralized water and the complex added and dissolved. Other addi- tiveε are added to obtain a homogeneous solution. Each spoon (~10 ml) contains 50 mg of nimesulide. Example 14. Composition of ointment with 10 mg/1 g nimesulide content: Complex of Example 5 9 g hydrophilic ointment 91 g Total 100 g The hydrophilic ointment base is melted at 50-60°C and the nime- sulide-salt complex is added under stirring to obtain a homoge¬ neously dispersed system. Under continuous stirring the ointment is cooled to room temperature and put into containers of 100 g. Example 15. Composition of a parenteral formulation containing 5 mg/ml of nimesulide sodium salt-gamma-CD complex: Complex of Example 6 500 mg sodium chloride 81 mg distilled water for injections ad 10 ml Proper volumes of the complex solution are filled into contai¬ ners with 50 mg nimesulide content each and lyophilized. Before use the lyophilized powder is dissolved with distilled water. Example 16. Composition of suppository containing 50 mg of nimesulide: Complex of Example 3 250 mg polyethylene glycol-suppository base ' 1250 mg Total 1500 mg The complex is homogenized with the melted suppository base and formulated to give suppositories. Example 17. Hard gelatine capsules used for in situ complexes: nimesulide-Na 53.5 mg 107 mg BCD 214 mg 428 mg Mg stearate 2.5 mg 5 mg Total 270 mg 540 mg The complex is formed when dissolving the capsule in acidic me¬ dium or after administration in the gastrointestinal tract.

Claims: CLAIMS 1. Inclusion complexes of nimesulide alkali and alkaline earth salts of general formula (I) where - A stands for an alkali and alkaline earth ion - with cyclodextrins and cyclodextrin derivatives. 2. Inclusion complex of nimesulide sodium, potassium, magnesium or calcium salt with B-cyclodextrin or gamma cyclodextrin the molar ratio of the salt to cyclodextrin being 1:1 or 1:2. 3. Inclusion complex according to claim 1 wherein the cyclodextrins and cyclodextrin derivatives are α, B and gamma cyclodextrins, and/or alkyl or hydroxyalkyl derivatives of cyclodextrin, prefe¬ rably methyl β-cyσlodextrins or hydroxypropyl-β- cyclodextrin. 4. Inclusion complex according to claim 1 wherein the nimesulide alkali is nimesulide sodium salt. 5. Process for the preparation of inclusion complexes of nimesulide alkali and alkaline earth salts and cyclodextrins or cyclodex¬ trin derivatives c h a r a c t e r i z e d b y reacting nime¬ sulide alkali and alkaline earth salts in the presence of water with cyclodextrins or cyclodextrin derivatives at pH 7 to 9.5 preferably at pH 7.5 to 8.5. 6. Process according to claim 5 c h a r a c t e r i z e d by using nimesulide alkali and alkaline earth salts formed in si¬ tu in the reaction mixture by adjusting the suspension of nime¬ sulide in water to a pH value of 7 to 9.5 preferably 7.5 to 8.5 by addition of alkali and alkaline earth hydroxides, alkali and alkaline earth carbonates, - hydrogen carbonates, - phosphates, preferably sodium hydroxide, disodium phosphate and/or sodium hydrogen carbonate or other buffers.

Inventors: GECZY JOSEPH, GECZY, JOSEPH
Applicants: CYCLOLAB LTD, EUROPHARMACEUTICALS SA, GECZY JOSEPH, CYCLOLAB LTD., EUROPHARMACEUTICALS S.A., GECZY, JOSEPH
Application year: 1994
Main classification: C08B37
IPC codes: A61K31, A61K47, A61P25, A61P29, C07C311, C08B37