HUMAN BRAIN PHOSPHODIESTERASE

Abstract: Isolated cDNA clones from human brain (frontal cortex) cDNA libraries that encode an unique subtype of the low Km, cAMP-specific phosphodiesterases (PDE IVs) are disclosed. Analysis of the distribution of hPDE IVB mRNA expression in various human tissues using a nonconserved fragment of the cDNA as a probe revealed a restricted pattern of expression, with an SIMILAR 4-kb mRNA detected in brain, heart, lung and skeletal muscle and not in placenta, liver, kidney or pancreas. Furthermore, an additional SIMILAR 5-kb hPDE IVB<-> related mRNA species was detected in brain tissue. Expression of hPDE IVB in a genetically-engineered PDE-deficient strain of the yeast Saccharomyces cerevisiae resulted in the overproduction of cAMP PDE activity which displayed the expected kinetic characteristics for a PDE IV: (1) low Km (4.3 mu M) for cAMP,2) high Km (>3 mM) for cGMP, and 3) sensitivity to rolipram (Ki = 0.085 mu M), a selective inhibitor of PDE IV. Recombinant hPDE IVB also bound [<3>H]R-rolipram saturably and with a high affinity. Analysis of [<3>H]R-rolipram binding data revealed curvilinear Scatchard plots, suggesting the presence of two non-interacting high affinity rolipram binding sites (Kd = 0.4 and 6nM) or a negatively cooperative interaction among multiple binding sites. This novel enzyme is particularly useful for screening candidate compounds for their ability to serve as potential anti-depressant, antiasthmatic or anti-inflammatory agents.

Description: Human Brain Phosphodiesterase Field of the Invention: This invention relates to cDNAs encoding phosphodiesterases (PDEs), their use in the recombinant production of the enzyme and the use of the PDE in drug screening. More specifically this invention relates to a unique subtype of a human, low K m , cAMP-specific phosphodiesterase (PDE IV) and its use in the screening of pharmaceutically useful substances. Background of the Tnvention: The recent identification and characterization of cDNA clones encoding several different mammalian PDEs has supported the cumulative biochemical evidence for the existence of multiple isozyme families as well as the number and tissue distribution of particular subtypes (Livi, G.P. £t al., Mol. Cell. Bio. 10:2678- 86 (1990); Colicelli, J. et al.. Proc. Natl. Acad. Sci: OJSA^ 86:3599-3603 (1989) and Davis ei al-, Proc. Natl. Acad. Sci.. (TJSAl 86:3604-08 (1989)). A particularly interesting isozyme family with respect to drug discovery is die PDE IV family. There is considerable evidence to suggest that this isozyme family represents a molecular target for a variety of therapeutic agents ranging from anti-depressants (Nicholson, CD. et al.. Trends in Pharm. Sci. 12:14-27 (1991)) to anti-asthmatic and anti-inflammatory agents (Torphy, T . and B.J. Undem, Thorax 46:512-23 (1991)). The cloning, expression and biochemical characteristics of hPDE IV^, an enzyme encoded by a cDNA obtained from a human monocyte library has been reported (Livi, CP.ej; aL supra ( 1990) and Torphy T. J. fit aL, Biol. Chem. 267:1798-1804 (1992)). The purpose of this invention is to provide cloned and characterized PDE IV subtypes expressed in human brain. Cyclic nucleotide phosphodiesterases (PDEs) consist of a family of enzymes that catalyze the hydrolysis of 3',5'-cyclic nucleotides, resulting in the formation of 5'-nucleotide metabolites. At least five distinct mammalian PDE isozyme families exist, each distinguished on the basis of a number of biochemical properties including 1) enzyme kinetics, 2) substrate selectivity, and 3) selective inhibition by various compounds. These isozyme families are defined as: I) the Ca 2+ /calmodulin-dependent PDEs; II) the cGMP-stimulated PDEs; in) the cGMP- inhibited PDEs; IV) the cAMP-specific PDEs and V) the cGMP-specific PDEs (Beavo, J.A. et pl.. Trends Pharmacol. Sci. 11:150-155 (1990) and Conti, M. ela Endocrine Rev. 12:218-234 (1991)). There is considerable interest in evaluating inhibitors of the low-K m , cAMP- specific PDEs (PDE IVs) as potential anti-inflammatory and anti-asthmatic drugs. As mentioned above, the cloning and expression of a cDNA that encodes a human PDE IV subtype expressed in monocytes (hPDE IV^) has been reported. This enzyme exhibited significant amino acid sequence homology to PDE IVs from rat brain (Colicelli, supra (1989)) and Drosophila (Chen, C-N. ei aL Proc. Nat'l. Acad. Sci. USA 83:9313-17 (1986)). Furthermore, the recombinant enzyme was overexpressed in both yeast and mammalian cells and defined as a PDE -TV based on its kinetic characteristics and sensitivity to isozyme-selective inhibitors. Recombinant hPDE IV^ possesses a low K m for cAMP (K m = 3.2 μM), a high K m for cGMP, and is inhibited by rolipram (Kj = 0.06 μM) but not by selective inhibitors of other PDE isozymes. It has been proposed that the anti-depressant activity of the PDE rV-selective inhibitor rolipram is associated with the inhibition of PDE IVs in the central nervous system. Rolipram binds with high affinity to rat brain homogenates and it has been assumed that this binding site represents either a catalytic or allosteric site within the PDE IV molecule itself. Accordingly, it has been recently established that recombinant hPDE IV^ possesses both catalytic activity and a high affinity (K<j = 2 nM) [ 3 H]-rolipram binding site (Toiphy T.J. et al.. J. Biol. Chem. 267:1798-1804 (1992)). Although the relationship between this high affinity binding site and the catalytic activity of hPDE IV ^ is not clear, it has been proposed that this site may represent either an allosteric site or the catalytic site on one of two distinct catalytic forms of the enzyme. It is of considerable interest to know if an additional PDE IV subtype is expressed in human brain, and if so, whether this subtype has biochemical characteristics similar to hPDE IV^. Of particular interest is the determination whether the high affinity rolipram binding site exists on PDE IV subtypes in addition to hPDE IVA- Although the mechanism of action of rolipram can be assessed biochemically using the available recombinant PDE IV enzymes derived from human monocytes and rat brain, a true pharmacological understanding of how PDE IV activity (as well as cellular cAMP content) regulates neurobiochemical processes is limited by lack of knowledge regarding PDE IV subtypes expressed in the human brain, if any. Accordingly, it is a purpose of this invention to provide isolated cDNA clones encoding PDE IV from human brain and to employ this valuable reagent in a screening protocol for the discovery of subtype-specific PDE IV inhibitors. Disclosed herein is the cloning of a cDNA from a human frontal cortex cDNA library that encodes a unique PDE IV subtype. This enzyme is designated as hPDE IVg according to the nomenclature of Beavo and Reifsnyder supra (1990). The cDNA product is defined as a type IV PDE based on its comparative amino acid sequence as well as PDE IV-selective inhibitors, the kinetic characteristics and die [3H] rolipram binding capacity of the recombinant enzyme. Surprisingly, this PDE IVg subtype exhibits a restricted tissue-type pattern of expression. Brief Description of the Invention This is invention provides an isolated nucleic acid molecule encoding a human PDE IV JJ. This invention also provides a human PDE IVg substantially free of other proteins of human origin. The invention also provides molecular reagents such as cloning and expression vectors for the recominbinant production of the PDE IVg enzyme. The invention also provides recombinant host cells capable of expressing the enzyme. The invention further includes a method for identifying ligands capable of binding to a PDE IVg enzyme comprising: contacting a PDE IVg enzyme with a plurality of candidate ligands labeled with an analytically detectable reagent under conditions sufficient for ligand binding and identifying those ligand candidates capable of enzyme binding by detecting the presence of a labeled ligand/enzyme complex. The invention also provides a method of screening compounds to identify those compounds which bind to a human PDE rVjj enzyme comprising contacting the enzyme with (a) a plurality of drug candidates in the presence of (b) an analytically detectable ligand known to bind to d e enzyme, under conditions that permit binding of (a) and (b) to the enzyme, and identifying those candidate compounds capable of enhancing or inhibiting the binding or interaction of the known ligand witii the enzyme.The invention also provides for detecting candidate compounds capable of inhibiting the catalytic activity of the enzyme by monitoring the effect of the compound on me ability of the enzyme to hydrolyze substrates (e.g., cAMP). The invention also provides biological screening assay for the detection of PDE IVg selective ligands comprising: (a) providing a PDE deficient host cell that exhibits a specific growth arrest phenotype associated with elevated c AMP levels; (b) transforming or transfecting said host cell with the plasmid capable of directing the expression of a PDE IVg enzyme and culturing the resultant recombinant host cell under conditions sufficient for the expression of PDE IVB enzyme and sufficient to generate a growth arrest response should d e expressed PDE IV β enzyme be inhibited; (c) contacting die recombinant host cell with a plurality of candidate compounds and (d) identifying tiiose compounds which are capable of inhibiting the enzyme and tiiereby unmasking the growth arrest phenotype. The invention also contemplates pharmacuetical compositions comprising a compound identified by any of die three abovementioned methods and a pharmaceutically acceptable carrier. The invention further includes antisense oligonucleotides having sequence capable of binding specifically with any sequence of an mRNA molecule which encodes die human PDE IVJJ so as to prevent die translation diereof. The invention also provides antibodies directed to die human PDE IVg The invention also provides a fusion protein comprising a PDE IVg domain and an cell surface localizing domain and a metiiod of screening compounds to identify ose compounds which bind to a human PDE IVg enzyme comprising contacting recombinant host cells expressing on the surface thereof the fusion protein with a plurality of drug candidates, under conditions to permit binding to me PDE IVβ domain, and identifying mose candidate drugs capable of enhancing or inhibiting the catalytic activity of the enzyme. Brief Peseription of the Figures Fig. 1 illustrates the composite nucleotide sequence of die hb-PDEl a and hb- PDEl cDNA clones with the predicted hPDE IVJJ amino acid sequence. The entire coding sequence, as well as the complete 5' and 3'-UTRs, are shown. Coordinates at d e right indicate nucleotide and amino acid positions. The amino acid sequence is shown starting from the putative translation initiation site (+1), and asterisks show the putative termination codon. Upper case letters correspond to clone hb-PDEl, whereas lower case letters correspond to nucleotides unique to clone hb-PDEl a (see Examples). The underlined region represents a non-conserved region (see Fig. 2) that was used as a probe for the Northern analysis shown in Fig. 5 A. The hb-PDEl cDNA extends from positions -283 to 2363, whereas hb-PDEl a extends from positions 1008 to 3609. The putative polyadenylation signal (5'-AATAAA-3') and poly (A) tract are bolded. The GenBank accession No. is M97515. Fig. 2 illustrates the deduced amino acid sequence alignment of representative mammalian low-K m cAMP-specific PDEs (PDE IVs). hPDE rVjj is the human brain PDE IV subtype reported here; hPDE IV^ is a human monocyte PDE IV; rPDE rV * A and rPDE IVB represent two distinct rat brain PDE IVs derived from die cDNA clones RD1 and DPD, respectively. Dashes indicate identical amino acids sequences; periods indicate sequence gaps included to maximize alignments. Fig. 3 illustrates the kinetics of hPDE IVB cAMP hydrolysis and inhibition by rolipram. PDE activity was assessed in yeast lysates 6 hr after hPDE IVg expression was induced. The data are representative of results from two separate preparations. Panel A, double-reciprocal plot of cAMP hydrolysis. The concentrations of cAMP used ranged from 0.03 to 50 μM. The K m for c AMP was calculated to be 4.3 ± 0.2 μM and d e V max was 11.3 nmol/mg protein/min. Data obtained witii the 10 highest concentrations of cAMP are shown on an expanded scale in die inset. Panel B, analysis of d e inhibitory effect of R-rolipram on cAMP hydrolysis by hPDE IVg. The symbols represent the actual experimental data whereas die lines were generated from the KINPAC kinetic analysis program and represent die theoretical position of data points for a competitive inhibitor witii a Kj = 0.085 μM. Fig. 4 illustrates Scatchard analysis of [^H] R-rolipram binding. Supernatant fractions of yeast cell lysates prepared 6 hr after induction of hPDE IVg expression were incubated witii various concentrations of [^H] R-rolipram (0.02-24 nM). Assays were conducted in die presence or absence of 1 μM unlabeled rolipram to define saturable binding. The results shown represent a Scatchard analysis of saturable [ 3 H] R-rolipram binding, which corresponded to more than 95% of the total binding. As analyzed by die Acufit computer program, the data best fitted a two-site model (F<0.01) with rolipram K^s = 0.4 and 6 nM. The data are representative of die results obtained witii two preparations. Fig. 5 illustrates the human tissue distribution of hb-PDEl -specific mRNA. A Northern blot containing poly (A) + mRNA extracted from various human tissues was probed witii a 32p_ι a beled PCR fragment of hb-PDEl corresponding to a nonconserved 3' region (see Fig. 1) in Panel A, stripped, and reprobed with a 32p. labeled β-actin cDNA in Panel B. Each lane contained 2 μg of poly (A) + RNA from the indicated human tissue. Size markers are in kb. Detailed Description of the Invention: In describing die present invention, the following terms will be employed, and are intended to be defined as indicated below. An "antigen" refers to a molecule containing one or more epitopes that will stimulate a host's immune system to make a humoral and/or cellular antigen-specific response. The term is also used herein interchangeably widi "immunogen." The term "epitope" refers to the site on an antigen or hapten to which a specific antibody molecule binds. The term is also used herein interchangeably with "antigenic determinant" or "antigenic determinant site." "Fusion protein" is a protein resulting from the expression of at least two operatively-linked heterologous coding sequences. The protein comprising a PDE IVg peptide and a second unrelated peptide sequence is an example of a fusion protein. A coding sequence is "operably linked to" another coding sequence when RNA polymerase will transcribe the two coding sequences into a single mRNA, which is then translated into a single polypeptide having amino acids derived from both coding sequences. The coding sequences need not be contiguous to one another so long as the expressed sequence is ultimately processed to produce die desired protein. "Recombinant" polypeptides refer to polypeptides produced by recombinant DNA techniques; i.e., produced from cells transformed by an exogenous DNA construct encoding d e desired polypeptide. "Syntiietic" polypeptides are those prepared by chemical synthesis. A "replicon" is any genetic element (e.g., plasmid, chromosome, virus) that functions as an autonomous unit of DNA replication in vivo: i.e., capable of replication under its own control. A "vector" is a replicon, such as a plasmid, phage, or cosmid, to which another DNA segment may be attached so as to bring about the replication of die attached segment. A "double-stranded DNA molecule" refers to the polymeric form of deoxyribonucleotides (bases adenine, guanine, thymine, or cytosine) in a double- stranded helix, both relaxed and supercoiled. This term refers only to the primary and secondary structure of the molecule, and does not limit it to any particular tertiary forms. Thus, this term includes double-stranded DNA found, inter alia, in linear DNA molecules (e.g., restriction fragments), viruses, plasmids, and chromosomes. In discussing die structure of particular double-stranded DNA molecules, sequences may be described herein according to die normal convention of giving only the sequence in the 5' to 3' direction along the nontranscribed strand of DNA (i.e., die strand having the sequence homologous to die mRNA). A DNA "coding sequence of or a "nucleotide sequence encoding" a particular protein, is a DNA sequence which is transcribed and translated into a polypeptide when placed under die control of appropriate regulatory sequences. A "promoter sequence" is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a coding sequence. For purposes of defining die present invention, die promoter sequence is bounded at die 3' terminus by a translation start codon (e.g., ATG) of a coding sequence and extends upstream (5' direction) to include die minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Wid in the promoter sequence will be found a transcription initiation site (conveniendy defined by mapping with nuclease Sl), as well as protein binding domains (consensus sequences) responsible for the binding of RNA polymerase. Eukaryotic promoters will often, but not always, contain "TATA" boxes and "CAT" boxes. Prokaryotic promoters contain Shine-Dalgarno sequences in addition to die -10 and -35 consensus sequences. DNA "control sequences" refers collectively to promoter sequences, ribosome binding sites, polyadenylation signals, transcription termination sequences, upstream regulatory domains, enhancers, and die like, which collectively provide for the expression (i.e., the transcription and translation) of a coding sequence in a host cell. A control sequence "directs die expression" of a coding sequence in a cell when RNA polymerase will bind die promoter sequence and transcribe the coding sequence into mRNA, which is then translated into d e polypeptide encoded by die coding sequence. A "host cell" is a cell which has been transformed or transfected, or is capable of transformation or transfection by an exogenous DNA sequence. A cell has been "transformed" by exogenous DNA when such exogenous DNA has been introduced inside die cell membrane. Exogenous DNA may or may not be integrated (covalendy linked) into chromosomal DNA making up die genome of the cell. In prokaryotes and yeasts, for example, the exogenous DNA may be maintained on an episomal element, such as a plasmid. Witii respect to eukaryotic cells, a stably transformed or transfected cell is one in which the exogenous DNA has become integrated into die chromosome so mat it is inherited by daughter cells tiirough chromosome replication. This stability is demonstrated by d e ability of the eukaryotic cell to establish cell lines or clones comprised of a population of daughter cell containing the exogenous DNA. A "clone" is a population of cells derived from a single cell or common ancestor by mitosis. A "cell line" is a clone of a primary cell that is capable of stable growth in vitro for many generations. Two DNA or polypeptide sequences are "substantially homologous" or "substantially die same" when at least about 93% (preferably at least about 95%, and most preferably at least about 98%) of the nucleotides or amino acids match over a defined length of the molecule, normally the entire length of die molecule. As used herein, substantially homologous also refers to sequences showing identity to the specified DNA or polypeptide sequence. DNA sequences that are substantially homologous can be identified in a Southern hybridization experiment under, for example, stringent conditions, as defined for tiiat particular system. Defining appropriate hybridization conditions is within d e skill of the art. ≤££, e.g.. "Current Protocols in Mol. Biol." Vol. I & II, Wiley Interscience. Ausbel al- (ed.) (1992). Protein sequences that are substantially the same can be identified by proteolytic digestion, gel electrophoresis and microsequencing. The term "functionally equivalent" intends that the amino acid sequence of the subject protein is one tiiat will elicit a catalytic response substantially equivalent to the specified PDE IVg peptide. A "heterologous" region of a DNA construct is an identifiable segment of DNA within or attached to another DNA molecule that is not found in association witii the od er molecule in nature. Thus, when the heterologous region encodes a receptor gene, die gene will usually be flanked by DNA tiiat does not flank die gene in die genome of the source animal. Another example of a heterologous coding sequence is a construct where the coding sequence itself is not found in nature (e.g., synthetic sequences having codons different from the native gene). Allelic variation or naturally occuiring mutational events do not give rise to a heterologous region of DNA, as used herein. A "high affinity" rolipram binding site is characterized by a Kj for rolipram of about lOnM or less, whereas a "low affinity" rolipram binding site is characterized by a Kj for rolipram of about lOOnM or greater. The term ligand as used herein means any molecule that can bind or interact with die PDE IVβ enzyme. When a ligand binds to the active site of die enzyme and is catalytically acted upon, the ligand is known as a substrate. Inhibitors are ligands which may or may not bind at die active site of die enzyme. This invention provides an isolated nucleic acid molecule encoding a human PDE IVg. Such an enzyme is defined by die criteria discussed above for members of this unique subtype. One means for isolating a human PDE IVg coding nucleic acid is to probe a human genomic or cDNA library with a natural or artificially designed probe using art recognized procedures (See for example: "Current Protocols in Molecular Biology", Ausubel, F.M., et al. (eds.) Greene Publishing Assoc. and John Wiley Interscience, New York, 1989,1992). One particularly useful probe for this purpose is a probe incorporating all, or a hybridizable fragment, of a DNA of the sequence disclosed herein as Seq. ID. No. 1. Alternatively, as describe hereinbelow, probes from PDE -TV^ may also be used. The isolated nucleic acid molecules obtained hereby may be used to obtain complementary copies of genomic DNA, cDNA or RNA from human, mammalian or other animal sources or to screen such sources for related sequences including transcriptional regulatory and control elements defined above as well as odier stability, processing, translation and tissue specificity-determining regions from 5' and/or 3' regions relative to the coding sequences disclosed herein. Additional coding sequences isolated by die procedures disclosed herein are considered to be substantially the same as the coding sequence given in Seq. ID. No.l if the additional sequence shares about 93% homology with the sequence of Seq. ID. No. 1. This invention also provides for an isolated protein which is die human PDE IVβ This enzyme is defined witii reference to the amino acid sequence listed in Seq. ID. No. 2 and includes variants witii a substantially homologous amino acid sequence but retaining the criteria of a PDE IVg enzyme identified herein. The proteins of this invention are preferably made by recombinant genetic engineering techniques. The isolated nucleic acids particularly the DNAs can be introduced into expression vectors by operatively linking the DNA to the necessary expression control regions (e.g. regulatory regions) required for gene expression. The vectors can be introduced into die appropriate host cells such as prokaryotic (e.g., bacterial), or eukaryotic (e.g., yeast or mammalian) cells by methods well known in the art (Ausubel et al.,_HiEEa).The coding sequences for the desired proteins having been prepared or isolated, can be cloned into any suitable vector or replicon. Numerous cloning vectors are known to those of skill in the art, and die selection of an appropriate cloning vector is a matter of choice. Examples of recombinant DNA vectors for cloning and host cells which they can transform include die bacteriophage λ (E. soli), pBR322 (E. £2ϋ), ρACYC177 (E. fifili), pKT230 (gram-negative bacteria), pGVl 106 (gram-negative bacteria), pLAFRl (gram-negative bacteria), pME290 (non-£. sadi gram-negative bacteria), pHV14 (£. βdi and Bacillus aώl-ili-ϋ. pBD9 (Bacillus), pϋ6i ( Streptomvces P UC6 (Streptomyces). YIp5 (Saccharomvces a baculovirus insect cell system, , YCpl9 (Saccharo vces ≤fig, generally. "DNA Cloning": Vols. I & π, Glover ei al- ed. IRL Press Oxford (1985) (1987) and; T. Maniatis £1 al- "Molecular Cloning" Cold Spring Harbor Laboratory (1982). The gene can be placed under die control of a promoter, ribosome binding site (for bacterial expression) and, optionally, an operator (collectively referred to herein as "control" elements), so that die DNA sequence encoding die desired protein is transcribed into RNA in the host cell transformed by a vector containing this expression construction. The coding sequence may or may not contain a signal peptide or leader sequence. The subunit antigens of the present invention can be expressed using, for example, the £. coli tac promoter or the protein A gene (spa) promoter and signal sequence. Leader sequences can be removed by die bacterial host in post-translational processing. See, e.g.. U.S. Patent Nos. 4,431,739; 4,425,437; 4,338,397. In addition to control sequences, it may be desirable to add regulatory sequences which allow for regulation of the expression of the protein sequences relative to the growth of the host cell. Regulatory sequences are known to those of skill in the art, and examples include tiiose which cause the expression of a gene to be turned on or off in response to a chemical or physical stimulus, including die presence of a regulatory compound. Other types of regulatory elements may also be present in die vector, for example, enhancer sequences. An expression vector is constructed so tiiat the particular coding sequence is located in die vector witii the appropriate regulatory sequences, the positioning and orientation of die coding sequence with respect to die control sequences being such that the coding sequence is transcribed under the "control" of the control sequences (i.e., RNA polymerase which binds to die DNA molecule at the control sequences transcribes the coding sequence). Modification of die sequences encoding die particular antigen of interest may be desirable to achieve this end. For example, in some cases it may be necessary to modify the sequence so that it may be attached to die control sequences with the appropriate orientation; i.e., to maintain die reading frame. The control sequences and other regulatory sequences may be ligated to the coding sequence prior to insertion into a vector, such as the cloning vectors described above. Alternatively, the coding sequence can be cloned directly into an expression vector which already contains the control sequences and an appropriate restriction site. In some cases, it may be desirable to add sequences which cause the secretion of the polypeptide from the host organism, with subsequent cleavage of the secretory signal. It may also be desirable to produce mutants or analogs of the receptors of interest. Mutants or analogs may be prepared by die deletion of a portion of the sequence encoding die protein, by insertion of a sequence, and/or by substitution of one or more nucleotides within the sequence. Techniques for modifying nucleotide sequences, such as site-directed mutagenesis, are well known to tiiose skilled in the art. See. e_jj„ T. Maniatis _l a supra: DNA Cloning. Vols. I and II, .sup∑a; Nucleic Acid Hybridization. sipm. A number of prokaryotic expression vectors are known in the art. ≤££, £, , U.S. Patent Nos. 4,578,355; 4,440,859; 4,436,815; 4,431,740; 4,431,739; 4,428,941; 4,425,437; 4,418,149; 4,411,994; 4,366,246; 4,342,832; S S S. alSΩ U.K. Patent Applications GB 2,121,054; GB 2,008,123; GB 2,007,675; and European Patent Application 103,395. Yeast expression vectors are also known in the art See. e.g.. U.S. Patent Nos. 4,446,235; 4,443,539; 4.430.428: see also European Patent Applications 103,409; 100,561; 96,491. pSV2neo (as described in J. Mol. Appl. Genet. 1:327-341) which uses the SV40 late promoter to drive expression in mammalian cells or pCDNAlneo, a vector derived from pCDNAKMol. Cell Biol. 7:4125-29) which uses the CMV promoter to drive expression. Botii tiiese latter two vectors can be employed for transient or stable(using G418 resistance) expression in mammalian cells. Insect cell expression systems, e.g., Drosophila. are also useful, see for example PCT applications US 89/05155 and US 91/06838 as well as EP application 88/304093.3. Depending on die expression system and host selected, die proteins of the present invention are produced by growing host cells transformed by an expression vector described above under conditions whereby the protein of interest is expressed. It is preferred to employ as a host a cell type that produced little or no endogenous PDE IV so as to make the identification and recovery of the recombinant form easier The protein is then isolated from the host cells and purified. If the expression system secretes die protein into growth media, die protein can be purified directly from die media. If the protein is not secreted, it is isolated from cell lysates or recovered from the cell membrane fraction. In certain cases were the protein is localized to the cell surface, whole cells or isolated membranes can be used as an assayable source of the desired gene product. The selection of die appropriate growth conditions and recovery methods are within die skill of the art An alternative metiiod to identify proteins of the present invention is by constructing gene libraries, using the resulting clones to transform I . coϋ and pooling and screening individual colonies using polyclonal serum or monoclonal antibodies to die desired enzyme. The proteins of the present invention may also be produced by chemical synthesis such as solid phase peptide synthesis, using known amino acid sequences or amino acid sequences derived from the DNA sequence of the genes of interest Such methods are known to those skilled in the art. Chemical synthesis of peptides is not particularly preferred. The proteins of the present invention or their fragments comprising at least one epitope can be used to produce antibodies, both polyclonal and monoclonal. If polyclonal antibodies are desired, a selected mammal, (e.g., mouse, rabbit, goat, horse, etc.) is immunized witii a receptor of the present invention, or its fragment, or a mutated receptor. Serum from the immunized animal is collected and treated according to known procedures. If serum containing polyclonal antibodies is used, the polyclonal antibodies can be purified by immunoaffinity chromatography or other known procedures. Monoclonal antibodies to the proteins of the present invention, and to the fragments thereof, can also be readily produced by one skilled in die art. The general methodology for making monoclonal antibodies by using hybridoma technology is well known. Immortal antibody-producing cell lines can be created by cell fusion, and also by other techniques such as direct transformation of B lymphocytes with oncogenic DNA, or transfection with Epstein-Baιτ virus. See, e.g.. M. Schreier ei aL "Hybridoma Techniques" (1980); Hammerling £l al., "Monoclonal Antibodies and T-cell Hybridomas" (1981); Kennett ei aL "Monoclonal Antibodies" (1980); ≤ss al≤Ω U.S. Patent Nos. 4,341,761; 4,399,121; 4,427,783; 4,444,887; 4,452,570; 4,466,917; 4,472,500; 4,491,632 and 4,493,890. Panels of monoclonal antibodies produced against the antigen of interest, or fragment thereof, can be screened for various properties; i.e., for isotype, epitope, affinity, etc. Monoclonal antibodies are useful in purification, using immunoaffinity techniques, of die individual antigens which they are directed against. Alternatively, genes encoding the monoclonals of interest may be isolated from the hybridomas by PCR techniques known in the art and cloned and expressed in die appropriate vectors. The antibodies of tiiis invention, whether polyclonal or monoclonal have additional utility in that tiiey may be employed reagents in immunoassays, RIA, ELISA, and die like. In other embodiments cell membrane fractions comprising the enzyme, partially purified supernatants containing the enzyme or isolated enzyme free or immobilized on solid supports may be used to measure binding of compounds to be tested. When recombinant cells are used for purposes of expression of the enzyme, it is preferred to use cells witii little or no endogenous enzyme activity so that binding if any is due to die presence of the expressed enzyme of interest. Preferred cells include yeast cells, particularly those of Saccharomyces cerevisiae that have been engineered to lack endogenous PDE activity. In a further embodiment a specific localization of PDE rVg can be achieved. For example a fusion protein can be made by fusing the enzyme of this invention with a protein domain which directs incorporation of such a fusion into the cell wall or cell membrane of the host cell. Such a domain, referred to here as a cell surface localizing domain, is capable, itself, or in association with accessory signal sequences known in the art, of directing the expression of the fusion protein and its integration into the host cell membrane or cell wall. It is most preferable to integrate the fusion protein so that die PDE IVg domain is displayed on die external surface of the host cell. In the compound screening embodiment of tiiis invention, die enzyme partially purified or in isolated, immobilized or cell bound form is contacted witii a plurality of candidate molecules and tiiose candidates are selected which bind to and interact with the enzyme (e.g., enzyme inhibitors). The binding or interaction can be measured direcdy by using radioactively labeled candidate of interest Alternatively, die candidate compounds can be subjected to a competition screening assays, in which a known ligand (e.g., rolipram), preferably labeled witii an analytically detectable reagent most preferably radioactivity, is introduced witii the compound to be tested and die compound's capacity to inhibit or enhance the binding of die labeled ligand is measured. Compounds are screened for tiieir increased affinity and selectivity to die enzyme class of interest In yet another approach advantage is taken of die catalytic activity of PDE IVg. Specifically, since PDE IVg hydrolyzes the 3'-phosphoester bond of cyclic nucleotides(e.g., cyclic AMP) to form 5'- monophosphate products, candidate molecules can be screened for their ability to inhibit die hydrolysis of cyclic nucleotides labeled with the appropriate analytically detectable reagent(e.g., radioactive, fluorometric or colorimetric agent). In yet another embodiment human PDE IVJJ functionally expressed in host cells, especially yeast may be employed in a rapid, high-diroughput screen to identify enyzme-selective inhibitors from sources such as natural products. Cells of Saccharomyces cerevisiae contain two genes that encode endogenous cAMP PDEs (Sass,P. fiia ., Proc. Natl. Acad. Sci. USA 83:9303-07 (1986); Wilson, R.B. et al., Mol. Cell. Biol. 8:505-510 (1988) and Nikawa, J.I. et al., Mol. Cell. Biol. 7:3629-36 (1987)). PDE-deficient mutants were constructed by reverse genetic techniques (gene disruption) according to die method of Rotiistein et al. (Cloning in Yeast. In: "DNA Cloning II: a practical approach", D.M. Glover ed., IRL Press, Washington D.C, pgs 45-66 (1990)) and were found to be viable, but to exhibit specific growth arrest phenotypes associated witii elevated cAMP content These phenotypes include: heat shock sensitivity, sensitivity to nitrogen starvation and die inability to grow on media containing sύboptimal carbon sources such as acetate. The details for the agar plate assay (i.e., media composition and conditions for assaying growth arrested phenotype) are described in Sass et al. supra: Wilson et Nikawa et al., supra and McHale, M.M. et al., Mol. Pharm. 39:109-113 (1991). Briefly, cells are grown in SC-Trp liquid medium for 2 days at 30° C, spotted onto SC-Trp agar medium containing 5mM cAMP and 150μM C11SO4 and incubated for 2 days at 30° C For heat shock, cells are replicated from the master plate onto two plates each containing the same medium as describe above, one of which is preheated to 55°C for 1 hour. Cells transferred to die preheated plate are incubated at 55°C for 5 minutes and tiien shifted to 30°C For monitoring growtii on acetate, the master plate is replicated to a plate containing the same medium except 2% potassium acetate instead of glucose. Growtii is scored after five days at 5 days at 30° C Functional expression of human PDE IV A or PDE IVJJ in genetically engineered PDE-deficient strains of S. cerevisiae reverses the aberrant phenotypes described above. Furthermore, under conditions for growth arrest rolipram, as well as certain other selective mammalian PDE IV inhibitors, are cytotoxic to PDE- deficient mutant cells expressing the human PDE IV under growth airest conditions. At the same time, rolipram has no killing effect on either wild-type yeast cells or on PDE-deficient cells expressing the human PDE IVs under normal growth conditions. These controls indicate that rolipram is not simply acting as an antifungal agent Instead, it is entering the yeast cells and inhibiting the activity of the human recombinant enzyme in the cells; this, in turn, results in an elevation of cAMP content and an unmasking of the concomitant growth arrest phenotype. Such a system provides die basis for a high throughput screen for PDE IV subtype-specific inhibitors. The source of compound for screening includes pure chemicals as well as natural products (e.g., fermentation broths from soil microorganisms, extracts from plants, animals and marine microorganisms). In such a screen compounds are identified by die ability to kill cells incubated under die prescribed conditions for growth airest (where cell viability depends on die functional expression (biological activity) of the human recombinant PDE IV) but at the same time, not affect wild-type cells. Although yeast cells may possess certain barriers (i.e., cell wall) that may make them less sensitive tiian mammalian cells to certain small molecules, such shortcomings are readily overcome by increasing the permeability of the yeast cell by for example, mutating genes that effect the synthesis of cell membrane components (e.g.., ergosterol) See for example, Gaber, R.F., et al., Mol, Cell, Biol 9:3447-3456 (1989). This system also offers an excellent method for rapidly assaying genetically engineered site specific mutants of human PDE IVg for functional catalytic activity in cells. Furthermore, this system allows for the direct selection of rolipram-resistant and catalytically active mutants of PDE IV which when characterized in terms of DNA sequence may reveal amino acid residues involved in compound binding. New compounds found to specifically inhibit the recombinant enzyme in vitro (see above) are then compared to rolipram or other reference compounds witii respect to tiieir capacity to unmask the inherent growth arrest phenotypes present in the yeast screening strains under appropriate culture conditions. In addition PDE IV inhibitors identified in natural products can be chemically purified and assayed for the ability to inhibit the recombinant enzyme(s) in vitro. In yet another aspect of this invention, the discovery of die heretofore unknown human PDE IVg subtype provides a new metiiod for rapidly screening subtype-specific inhibitors. Accordingly, die PDE IV A and B subtypes are expressed in separate PDE-deficient yeast strains and candidate compounds are screened for their ability to inhibit one but not both die enzyme subtypes. Thus compounds may be identified tiiat inhibit the monocyte A form but not the brain B form of PDE IV. This invention also contemplate pharmaceutical compositions comprising compounds when identified by die above metiiods and a pharmaceutically acceptable carrier. Pharmaceutical compositions of proteineous drugs of this invention are particularly useful for parenteral administration, i.e., subcutaneously, intramuscularly or intravenously. The compositions for parenteral administration will commonly comprise a solution of the compounds of die invention or a cocktail thereof dissolved in an acceptable carrier, preferably an aqueous carrier. A variety of aqueous carriers may be employed, e.g., water, buffered water, 0.4% saline, 0.3% glycine, and die like. These solutions are sterile and generally free of particulate matter. These solutions may be sterilized by conventional, well known sterilization techniques. The compositions may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions such as pH adjusting and buffering agents, etc. The concentration of die compound of the invention in such pharmaceutical formulation can vary widely, i.e., from less than about 0.5%, usually at or at least about 1% to as much as 15 or 20% by weight and will be selected primarily based on fluid volumes, viscosities, etc., according to the particular mode of administration selected. Thus, a pharmaceutical composition of die invention for intramuscular injection could be prepared to contain 1 ml sterile buffered water, and 50 mg of a compound of die invention. Similarly, a pharmaceutical composition of the invention for intravenous infusion could be made up to contain 250 ml of sterile Ringer's solution, and 150 mg of a compound of die invention. Actual methods for preparing parenterally administrable compositions are well known or will be apparent to those skilled in the art and are described in more detail in, for example, Remington's Pharmaceutical Science. 15tiι ed., Mack Publishing Company, Easton, Pennsylvania. The compounds described herein can be lyophilized for storage and reconstituted in a suitable carrier prior to use. This technique has been shown to be effective with conventional proteins and art-known lyophilization and reconstitution techniques can be employed. In situations where the identified compound is non-proteineous, it may be administered alone or in combination with pharmaceutically acceptable carriers . The proportion of which is determined by die solubility and chemical nature of the compound, chosen route of administration and standard pharmaceutical practice. For example, they may be administered orally in the form of tablets or capsules containing such excipients as starch, milk sugar, certain types of clay and so forth. They may be administered sublingually in the form of troches or lozenges in which the active ingredient is mixed with sugar and corn syrups, flavoring agents and dyes; and tiien dehydrated sufficiendy to make it suitable for pressing into a solid for. They may be administered orally in the form of solutions which may be injected parenterally, that is, intramuscularly, intravenously or subcutaneotisly. For parenteral administration they may be used in the form of a sterile solution containing other solutes, for example, enough saline or glucose to make the solution isotonic. The physician will determine die dosage of die present therapeutic agents which will be most suitable and it will vary with die form of administration and the particular compound chosen, and furthermore, it will vary with die particular patient under patient under treatment. He will generally wish to initiate treatment with small dosages substantially less than the optimum dose of die compound and increase the dosage by small increments until die optimum effect under die circumstances is reached. It will generally be found that when die composition is administered orally, larger quantities of die active agent will be required to produce die same effect as a smaller quantity given parenterally. The compounds are useful in the same manner as other PDE IVg- effecting agents and die dosage level is of die same order of magnitude as is generally employed with these other therapeutic agents. The therapeutic dosage will generally be from 1 to 10 milligrams per day and higher although it may be administered in several different dosage units. Tablets containing from 0.5 to 10 mg. of active agent are particularly useful. Depending on die patient condition, the pharmaceutical composition of the invention can be administered for prophylactic and or tiierapeutic treatments. In therapeutic application, compositions are administered to a patient already suffering from a disease in an amount sufficient to cure or at least partially arrest the disease and its complications. In prophylactic applications, compositions containing the present compounds or a cocktail thereof are administered to a patient not already in a disease state to enhance the patient's resistance. Single or multiple administrations of the pharmaceutical compositions can be carried out witii dose levels and pattern being selected by die treating physician. In any event die pharmaceutical composition of the invention should provide a quantity of the compounds of die invention sufficient to effectively treat the patient The nucleic acid embodiment of this invention is particularly useful in providing probes capable of specific hybridization with human PDE IVjj sequences. Probing technology is well known in the art and it is appreciated tiiat the size of the probes can vary widely but it is preferred tiiat the probe be at least 15 nucleotides in length. It is also appreciated that such probes can be and are preferably labeled witii an analytically detectable reagent to facilitate identification of die probe. Useful reagents include but are not limited to radioactivity, fluorescent dyes or enzymes capable of catalyzing the formation of a detectable product. This invention contemplates, for example using PDE IVg encoding probes in the diagnostic evaluation of disease states characterized by an abnormal, i.e., increased or decreased level of PDE I Vβ gene expression. Alternatively, the probes can be used to identify individuals carrying chromosomal or molecular mutations in the gene encoding die enzyme. Depending on die conditions employed by die ordinary skilled artisan, the probes can be used to identify and recover additional examples of die PDE IVjj enzyme from other cell types and individuals. As a general rule the more stringent the hybridization conditions the more closely related genes will be that are recovered. Also within the scope of this invention are antisense oligonucleotides predicated upon die sequences disclosed herein for the PDE TVβ enzyme. Synthetic oligonucleotides or related antisense chemical structural analogs are designed to recognize and specifically bind to a target nucleic acid encoding die receptor gene and inhibit gene expression, e.g., the translation of the gene when die target nucleic acid is mRNA. Although not wishing to be bound to a particular theory for the mechanism of action of antisense drugs, it is believed tiiat such drugs can act by one or more of the following mechanisms: by binding to mRNA and inducing degradation by endogenous nucleases such as RNase I or by inhibiting the translation of mRNA by binding to regulatory factors or ribosomal components necessary for productive protein synthesis. Additionally die antisense sequences can be used as components of a complex macromolecular arrays in which the sequences are combined witii ribozyme sequences or reactive chemical groups and are used to specifically target mRNAs of interest and degrade or chemically modify said mRNAs. The general field of antisense technology is illustrated by die following disclosures which are incorporated herein by reference for purposes of background (Cohen, J.S., Trends in Pharm. Sci. 10:435(1989) and Weintraub, H.M. Scientific American Jan.0 90^ at page 40). This invention also contemplates antibodies, monoclonal or polyclonal directed to epitopes corresponding to amino acid sequences disclosed herein from the PDE IVg enzyme. Particularly important regions of the enzyme for immunological purposes are those catalytic and allosteric domains of the enzyme. Antibodies or fragments thereof directed to tiiese regions are particularly useful in diagnostic and therapeutic applications because of there effect upon enzyme-ligand interaction. Methods for die production of polyclonal and monoclonal antibodies are well known, see for example Chap. 11 of Ausubel al- .supra). This invention also provides pharmaceutical compositions comprising an effective amount of antibody or antigen binding fragments thereof directed against the PDE Γ * B enzyme to block binding of naturally occurring ligands to tiiat enzyme in order to treat or ameliorate disease states associated witii inappropriate enzyme activation. In its diagnostic embodiment the PDE IVg enzyme can be detected by contacting it with antibodies of this invention and measuring the antibody/enzyme complex. When die antibody is labeled witii an analytically detectable reagent such a radioactivity, fluorescence, or an enzyme, the antibody can be use to detect die presence or absence of the enzyme and/or its quantitative level. As disclosed herein below, screening a human frontal cortex library revealed die existence of a unique cDNA that encodes a protein with 76% amino acid sequence identity to hPDE IV^. Expression of this cDNA in PDE-deficient yeast cells indicates that the gene product possesses characteristics consistent with those of the PDE -TV enzyme family. Specifically, the recombinant enzyme, designated hPDE rVβ, has a cAMP K m = 4μM and exhibits very poor activity with cGMP as a substrate. Moreover, the activity of this enzyme is inhibited by rolipram (Kj = 0.085 μM) and other PDE IV-selective inhibitors, but not by compounds that selectively inhibit other PDE isozymes. Similar to the results obtained witii hPDE IVA, rolipram does not inhibit hPDE IVg activity in a purely competitive fashion. While not wishing to be bound by any particular explanation, this could reflect a kinetically complex mechanism of inhibition by rolipram (e.g., allosteric interaction) or could indicate tiiat the recombinant hPDE IVβ exists in two distinct, catalytically active, non-interconvertable forms, both of which can be inhibited competitively by rolipram but with significandy different Kjs. Based upon the limited evaluation of hPDE IVg carried out thus far, it appears that the kinetic properties of this subtype are similar to hPDE rV^. As observed witii hPDE IV A, hPDE IVg catalytic activity and high affinity rolipram binding are coexpressed. Thus, high affinity rolipram binding is a property of both hPDE IV^ and hPDE IVg. Interestingly, at least two classes of kinetically distinct high affinity rolipram-binding sites exist on hPDE IVg (K^s = 0.4 and 6 nM), whereas only one class (K^ = 2 nM) is present on hPDE IV j . Valuable information regarding enzyme structure and function can obtained from comparisons of the predicted primary amino acid sequences of different PDEs. All of die well-studied mammalian PDEs exhibit a common structure consisting of a highly conserved 270-300 amino acid sequence within the central core of the molecule corresponding to the catalytic domain, flanked by variable N-terminal and C-terminal extensions. Based on a considerable amount of biochemical data, die conserved region contains the information for catalytic activity, whereas the nonconserved regions may dictate subtype-specificity in terms of regulation of enzymatic activity, cellular distribution or subcellular localization. Alignment of the primary amino acid sequence of hPDE IVg with tiiat of one of the rat brain enzymes (rPDE IVg in Fig. 2) shows that they are nearly identical (from residues 38 to 564); tiiis conservation of sequence includes die C-terminal end of each molecule. In contrast, comparison of the hPDE IV A and hPDE ΓVJJ subtypes reveals striking sequence divergence, especially at the N-terminal and C-terminal ends. This suggests that tiiere is a greater degree of sequence conservation between die "same" isozyme subtype from different species than among "different" subtypes from the same species. Although significant structural similarity in the conserved regions was observed between of hPDE TVj (which is known to be expressed in human monocytes), and one of die enzymes cloned from a rat brain cDNA library (rPDE IV A in Fig. 2), it is impossible to draw a conclusion regarding die functional relationship between these two subtypes considering die extensive sequence divergence present at their N-terminal and C-terminal ends. The prominent characteristic that separates these two subtypes from the others, however, is the long C-terminal domain. It is quite possible the two subtypes are functionally equivalent, and tiiat die rat brain enzyme is not really brain-specific. That is, the mRNA template for the rat brain cDNA could actually have come from contaminating blood monocytes. Unfortunately, the analysis of the tissue-distribution of die rat PDE r transcripts, which showed expression in all tissues examined, used full- length cDNAs as probes and dierefore did not differentiate the expression of various subtypes (Swinnen, J.V. fil aL E∑ΩS EaiL Acad, S≤ 1ZSΔ 86:5325-5329 (1989)). Using a DNA probe consisting of nonconserved sequences, the human tissue distribution of hPDE IVg mRNA was analyzed herein and found to exhibit a restricted pattern of expression. An ~4-kb message was easily detected in brain, as well as in three other tissues, but not in placenta, liver, kidney and pancreas. In brain, we observed an additional ~5-kb mRNA species which may represent unprocessed mRNA. Alternatively, the identification of two hPDE IVjj-related mRNAs in brain tissue may correspond to differentially spliced transcripts expressed from the same gene, or instead, may indicate die presence of another as yet unidentified gene. More important is the differential response of hPDE IV^ and hPDE IVg to inhibitors. Predicated upon such a response, subtype specific drug screening can be developed. In one embodiment of this invention a screening protocol may be conducted as outline hereinabove. Example 1 This example provides die details of the cloning, expressing and characterization of the PDE IVg of this invention. Experimental Procedures Isolation of cDNA~A commercially prepared human frontal cortex cDNA library constructed in λZAP (Stratagene; average insert size - 1.5 kb) was screened witii a 3 p-iabeled (random-primed; Pharmacia) 1.8-kb Smal fragment of the human monocyte cDNA clone hm-PDEl (formerly hPDE-1) which encodes a PDE IV. Hybridizations were carried out at 65°C in 6X SCP (0.6 M NaCl, 0.81 M Na2HPO 6 mM EDTA, pH 6.2), 10% dextran sulfate, 5X Denhardt's (0.01% ficoll, 0.01% polyvinylpyirolidone, 0.01% bovine serum albumin), 0.1% SDS and 100 mg/ml salmon sperm DNA. Filters were washed in 2X SCP, 0.1% SDS at 65° C and autoradiographed. A number of clones were isolated and partially characterized by restriction mapping. DNA sequencing of one 2.7 -kb clone (hb- PDEla) by the method of Sanger, F. fit aL (Proc. Natl. Acad. Sci. USA 74:5463-67 (1977)) revealed that it contained a truncated ORF, predicting a protein witii homology to only the 3' half of PDE protein encoded by hm-PDEl. A 0.69-kb Ndel-Sphl fragment, containing the majority of the ORF of clone hb-PDE 1 a was then used to screen a custom cDNA library prepared at Stratagene from human frontal cortex tissue (also in λZAP). This library was prepared using methods to insure large insert sizes. Briefly, the RNA was denatured in the presence of methyl mercury and converted to cDNA using 80% oligo dT and 20% random priming. The cDNA was then size-selected for inserts of 1.5-kb and greater by agarose gel electrophoresis. Labeling, hybridization and washing conditions were identical to those stated above. This screen yielded 11 clones which were analyzed by restriction mapping. The DNA sequence of the largest clone, hb-PDEl, was determined and found to overlap the sequence of hb-PDE la by 1.35 kb, and extend 1.28 kb further upstream (5'); the total size of hb-PDEl is 2.63 kb. Thus, die composite of hb-PDEla and hb-PDEl represent a cDNA containing both 5' and 3' UTRs. hb-PDEl alone encodes a full-length PDE IV-related protein (called hPDE rV β ) as judged by an alignment of its primary amino acid sequence with that of other PDE IV subtypes (see Figure 2). Northern Analysis - An "hb-PDEl -specific" DNA probe was generated by PCR (see: Mullis, K.B. and F.A. Faloona, Msi Ωf Enz» 155:335-50 (1987)) using the following oligonucleotide primers: 5'- GGGGCTCGAGGAGGGACACACGTATTT- CAGCAGCACAAAG-3' (Seq. ID No:3)and 3'- CTCACTTGAGTGACTGATTATTGAAGT- AAAGAGCTCGGGG-5'.(Seq. ID No:4) This fragment was subcloned into pGEM7 (Promega) using the unique Xholsites (underlined). The Xhol fragment was gel purified, 3 p-i a 5 e i e d (random-primed; Pharmacia), and hybridized to a Northern blot containing poly (A) + RNA extracted from multiple human tissues (Clonetech); hybridization conditions were: 42°C in 5X SSPE (0.75M NaCl, 0.2 M NaH 2 Pθ4-H2θ, 5mM Na 2 EDTA, pH7.4), 10X Denhardt's, 100 mg/ml salmon sperm DNA, 50% formamide and 2% SDS. The blot was washed in 0.1X SSC (15 mM NaCl, 1.5 mM sodium citrate, pH7.0), 0.1% SDS at 50°C and autoradiographed. The same blot was stripped with boiling water and reprobed with a 2-kb human β-actin-encoding cDNA (see: Cleveland, D.W. ei aL £sll 20:95-105 (1980)). Labeling, hybridization and washing conditions were the same as above. Expression of hPDE IVg in Saccharomyces cerevisiae- The hb-PDEl cDNA was engineered for expression in yeast as follows: the 2.08-kb Xhol (5' polylinker) - Ncol fragment of pBluescript/hb-PDEl was subcloned into die unique Xhol and Ncol polylinker sites of the yeast expression plasmid pl38NB (McHale, M.M. et al. Mol. Pharmacol. 39:109-113 (1991)). The 5' UTR of hb-PDEl was then deleted by removing the 1.1 -kb Xhol- PvuII fragment and replacing it with a 0.79-kb Xhol- PvuII hb-PDEl fragment generated by PCR in which a Xhol site was engineered just upstream of the initiating methionine codon. The oligonucleotide primers used for PCR were: 5'- GCιGGGCTCGAGAATGAAGGAGCACGGGGGCACCTTCAGTAGC-3' (Seq. ID No:5)and 3'-GACGGTAAAAACCιrCQΔCGGTAGGTACTGC-5' (Seq. ID No:6). The PCR-generated portion of this plasmid (pl38NB/hb~PDEl) was sequenced and found to contain one base pair change that did not affect the amino acid sequence. pl38NB/hb-PDEl contains the TRP1 selectable marker and partial 2μ sequences for maintenance at high copy number, with hb-PDEl expression driven by the copper inducible CUPl gene promoter. The plasmid was introduced into the PDE-deficient £_. cerevisiae strain GL62 (isogenic to strain GL61; described in McHale et al. (supral (1991)) using the lithium acetate method (Ito, H., £1 aL, L Bacteriol. 153:163-168 (1983)). Trp+ prototroph were isolated and grown aerobically at 30°C to an A540 = 1.0 in synthetic complete medium lacking tiyptophan. PDE expression was induced by die addition of 150 μM C11SO4. Cells were harvested at 6 hr and 100,000 x g supernatants were prepared as previously described by McHale et al. (supra). PDE Assays and Inhibitor Studies - PDE activity was determined in 100,000 x g supernatant fractions of yeast cell lysates. Briefly, the reaction was initiated by the addition of an aliquot of the yeast supernatant fraction to 0.1 ml (final volume) of a reaction mixture containing (final concentrations) 50 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 1 μM [ 3 H] cAMP (2000 dpm pmol) and 0.05% BSA. To determine recovery, 50 μM 5'-[^ C] AMP (400 dpm/nmol) was added to each assay tube after the reaction was terminated. For studies in which die IC50S of various inhibitors were determined, compounds were present in the reaction mixture at concentrations that varied over three orders of magnitude. Reactions were conducted at 30°C for 30 min. The reactions were terminated, 5'-adenosine monophosphate was isolated, and PDE activity was determined as previously described (Torphy, T.J. and L.B. Cieslinski. Mol. Pharmacol. 37:206-214 (1990)). All assays were conducted in die linear range of reaction within which no greater than 10% of the initial substrate is hydrolyzed. cGMP hydrolysis was also assayed as described by Torphy, T.J. and Cieslinski (supra). Protein concentrations were determined as previously described (Lowry, O.H. et al. J. Biol. Chem. 193:265-75 (1951)). Rolipram Binding Assay — [ 3 H] R-Rolipram binding was assessed by modification of the method of Schneider and co-workers (Eur. L Pharmacol. 127:105-115 (1986)). For competition binding experiments, the reaction was conducted at 30°C in 0.5 ml of a standard reaction mixture containing (final concentrations): 50 mM Tris-HCl (pH7.5), 5 mM MgCl2, 50 μM 5'- AMP, 2 nM [ 3 H] R-rolipram (5.7 x 10 4 dpm/pmol) and 0.05% BSA. For saturation binding experiments, the concentration of [ 3 H] R-rolipram was varied from 0.02-24 nM. Nonspecific binding was defined in the presence of 1 μM unlabeled rolipram and was consistendy less tiian 5% of total binding. The reaction was stopped after 1 h by the addition of 1 ml ice-cold reaction buffer (without [ 3 H] R-rolipram) and rapid vacuum filtration (Brandel Cell Harvester) through Whatman GF/B filters that had been soaked in 0.3% polyethylenimine. The filters were washed witii an additional 5 ml of cold buffer, dried and counted via liquid scintillation spectrometry. Determination of Kinetic and Binding Parameters—For determination of Vmax, K m , and Kj, the concentration of cAMP or cGMP was varied while the amount of 3 H-labeled cyclic nucleotide per assay was kept constant. Appropriate corrections were made for the changes in specific activity of the substrate. Kinetics were analyzed witii a KINPAC computer program described by W.W. Cleland (Meth. ofEnzvmol. 63:103-38 (1979)), using a nonlinear least-squares regression analysis. Analysis of [ 3 H] R-rolipram binding experiments, including determination of multiple K^ and V max values, was carried out using the Acufit computer program (Beckman Instruments, Fullerton, CA). Statistical comparisons between one-site and two-site fits were conducted via F test. Inhibitors and radioligand— R- and (S)-rolipram were synthesized by Dr. Sigfried Christensen and colleagues (SmithKline Beecham Pharmaceuticals, King of Prussia, PA); [ 3 H] R-rolipram (5.7 x IO 4 dpm/pmol) was prepared by Dr. Richard Heys and colleagues (SmithKline Beecham Pharmaceuticals, King of Prussia, PA); denbufylline was obtained from SmithKline Beecham Pharmaceuticals (Epsom, United Kingdom); Ro 20-1724 was purchased from BioMol (Plymouth Meeting, PA); zaprinast and siguazodan (SK&F 94836) were synthesized by Dr. William Coates and colleagues (SmithKline Beecham Pharmaceuticals, Welwyn, United Kingdom). RESULTS Cloning and Nucleotide Sequence of a Human Brain PDE IV cDNA— A DNA fragment from the conserved region of a human monocyte cDNA shown to encode a low-K m cAMP-specific PDE was used to probe a human frontal cortex cDNA library. Numerous clones were obtained, characterized by restriction mapping, and die DNA sequence of one 2.7-kb clone was determined. This clone (hb-PDE la) contained sequence information which was homologous to a large 3' portion of die hPDE IV A CDNA. hb-PDE la was then used to probe a second cDNA library which was custom synthesized from human frontal cortex. Again, numerous clones were obtained, and based on restriction analysis we determined die DNA sequence of one clone containing the longest 5' extension. Fig. 1 shows the composite nucleotide sequence of tiiese two cDNAs. hb-PDEl extends from positions -283 to 2363, whereas hb-PDEla extends from positions 1008 to 3609. The sequence contains an ORF of 1692 bp in length, which predicts a 564 amino acid protein with a calculated molecular mass of 64,202 (minus the N-terminal methionine). The ORF is flanked by 5' and 3' untranslated sequences of 283 and 1907 bp, respectively. The 3' UTR contains a putative polyadenylation signal (5'-AATAAA-3') at position 3572, followed by a poly (A) tract. Analysis of the Predicted hb-PDEl Protein Sequence-Fig. 2 shows an alignment of the hPDE IVg primary amino acid sequence with the sequences of three other cloned mammalian PDE IVs, one from human monocytes (hPDE IV^), and two from rat brain (rPDE IV j ; rPDE iVg). Recombinant forms of all of these other enzymes have been found to exhibit die biochemical properties indicative of a type IV PDE. There is striking homology between the hPDE IVg protein and all of the other PDE IVs, especially within the central core of the molecule which has been proposed to contain the catalytic domain. Interestingly, the sequence of hPDE IVB is significantly more homologous to the sequence of one of the rat brain proteins (rPDE IVg; 92% identity over 562 amino acids; 98% identity over 526 amino acids starting at residue 38 of hPDE IVg) than to the PDE rv from human monocytes (hPDE IV ; 76% identity over 538 amino acids) (Fig. 2). In fact, the protein sequences of hPDE IVβ and rPDE IVg precisely align at their C-terminal ends. The relationship between these two proteins is made even more apparent by the perfect alignment of sequence gaps inserted to accurately compare all four proteins (Fig. 2). It is also quite apparent that, whereas hPDE IVg and rPDE IVg are highly homologous to one another, the sequence of the hPDE IV A protein is very similar to that of the other rat brain protein, rPDE IV^ (83% identity over 504 amino acids). Both hPDE IV A and rPDE IV A contain an insertion near the N- terminal end (adjacent to residue 131 of hPDE IVg) and a long C-terminal extensions not found on the other two proteins. Fig. 2 also shows that many of the differences between the hPDE IVg and both the hPDE IV A and rPDE IV^ involve residues tiiat are conserved among the latter two proteins. Thus, there is a lesser degree of protein sequence conservation between the two human PDE IVs than between two PDE IVs derived from die brain tissues from different species. Expression of hPDE IVg in Yeast and Evaluation of its Catalytic Activity-In order to biochemically characterize the recombinant hPDE IVg enzyme, the hb- PDEl cDNA was engineered for overexpression in the yeast £. cerevisiae. This was done using a genetically-engineered PDE-deficient strain of yeast which contained genomic disruptions of the two genes encoding endogenous cAMP-specific PDEs. Soluble fractions of yeast cells containing an expression plasmid for hPDE ΓVJJ (pl38NB/hb-PDEl) accumulated an appreciable amount of cAMP hydrolyzing activity following induction (2.0 nmol/min per mg of protein in the presence of 1 μ M[ 3 H]cAMP), compared to a negligible amount of activity seen in samples from cells containing plasmid lacking the cDNA insert (pl38NB). The hPDE IV activity expressed in yeast exhibited standard Michaelis-Menton behavior with respect to catalytic activity and a high affinity for cAMP, with a K m of 4.3 μM and a V max =11.3 nmol/mg protein/min (Fig. 3 A). Furthermore, R-rolipram potently inhibited catalytic activity (Kj=0.085 μM), although the inhibition observed did not appear to be strictly competitive (Fig. 3B). The recombinant enzyme was also inhibited by two other PDE IV inhibitors, Ro 20-1724 (IC5Q=2.2 μM) and denbufylline (IC50=0.5 μM), but not by siquazodan or zaprinast (IC5os>30 μM), inhibitors of PDE in and PDE V, respectively. No detectable hydrolysis of cGMP was noted, even at substrate concentrations as great as 10 mM. [ 3 H] R-rolipram Binding— A typical Scatchard analysis of saturation binding experiments with [ 3 H] R-rolipram is shown in Fig. 4. The Scatchard plots were consistently curvilinear and best fitted a two-site model rather tiian a one-site model (F <0.01). Assuming that the curvilinear Scatchard plots reflected two distinct binding sites rather than a negatively cooperative interaction, K^s of 0.4 nM and 6 nM were calculated witii the higher affinity sites representing approximately one- third of die total sites. A comparison of the kinetic behavior and [ 3 H] rolipram-binding characteristics of hPDE IV A and hPDE IVβ is shown in Table 1. The kinetic characteristics of these subtypes are virtually identical with respect to their catalytic activity against c AMP and cGMP, as well as their sensitivity to R-rolipram. However, unlike hPDE IVg, which appears to contain more than one high affinity rolipram-binding site (Fig. 4), hPDE IV A possesses only a single class of non- interacting high affinity binding sites. TABLE I Comparison of the kinetic behavior and [ 3 H] R-rolipram binding characteristics of hPDE IV A and hPDE IV B Subtype K^ R-Rolipram Kj pH] R-Rolipram K d cAMP cGMP μM nM nM hPDE IVA a 3-1 >100 60 1 hPDE IV B 4.3 >10,000 85 0.4, 6 a Data from Torphy, T.J. et al.. J. Biol. Chem. 267:1798-1804 (1992). mRNA Tissue Distribution-mRNA transcripts corresponding to hb-PDEl were detected in only half of the human tissues surveyed (Fig. 5 A). Northern blot analysis of poly (A) + RNA derived from various human tissues revealed die presence of an ~4-kb mRNA in brain, heart, lung and skeletal muscle, with no mRNA detected in placenta, liver, kidney and pancreas. An additional ~5-kb mRNA was detected in brain, present in approximately equal abundance relative to the ~4- kb mRNA (Fig. 5A). For these studies, a probe which consisted of an hb-PDEl fragment representing a nonconserved portion of the coding region plus a portion of the 3' UTR (underlined sequence in Fig. 1) was used. The blot was stripped and reprobed with a 2-kb human β-actin cDNA as a control for the presence of RNA in each lane. Fig. 5B show that the amount of poly (A) + RNA loaded from each tissue was roughly equivalent, with the exception of lung which was apparendy overloaded; the lower ~1.8-kb β-actin band observed in heart and skeletal muscle represents a second muscle-specific form of the protein. SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Livi, George P. McLaughlin, Megan M. Torphy, Theodore J. (ii) TITLE OF INVENTION: Human Brain Phosphodiesterase (iii) NUMBER OF SEQUENCES: 6 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: SmithKline Beecham Corporation (B) STREET: Corporate Patents/ P.O.Box 1539 (C) CITY: King of Prussia (D) STATE: PA (E) COUNTRY: USA (F) ZIP: 19406-0939 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: US (B) FILING DATE: (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: Jervis, Herbert H. (B) REGISTRATION NUMBER: 31,171 (C) REFERENCE/DOCKET NUMBER: P50200 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (215) 270-5019 (B) TELEFAX: (215) 270-5090 (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3890 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: CDNA (iii) HYPOTHETICAL: NO (iv) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (F) TISSUE TYPE: Brain (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 282..1973 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: GGCACGAGCC TAAAGAACCT CGGGATGACT AAGGCAGAGA GAGTCTGAGA AAACTCTTTG 60 GTGCTTCTGC CTTTAGTTTT AGGACACATT TATGCAGATG AGCTTATAAG AGACGCTTCC 120 CTCCGCCTTC TTCCTCAGAG GAAGTTTCTT GGTAGATCAG GCACACCTCA TCCAGGCGGG 180 GGGTTGGGGG GAAACTTGGC ACCAGCCATC CCAGGCAGAG CACCACTGTG ATTTGTTCTC 240 CTGGTGGAGA GAGCTGGAAG GAAGGAGCCA GCGTGCAAAT A ATG AAG GAG CAC 293 Met Lys Glu His 1 GGG GGC ACC TTC AGT AGC ACC GGA ATC AGC GGT GGT AGC GGT GAC TCT 341 Gly Gly Thr Phe Ser Ser Thr Gly lie Ser Gly Gly Ser Gly Asp Ser 5 10 15 20 GCT ATG GAC AGC CTG CAG CCG CTC CAG CCT AAC TAC ATG CCT GTG TGT 389 Ala Met Asp Ser Leu Gin Pro Leu Gin Pro Asn Tyr Met Pro Val Cys 25 30 35 TTG TTT GCA GAA GAA TCT TAT CAA AAA TTA GCA ATG GAA ACG CTG GAG 437 Leu Phe Ala Glu Glu Ser Tyr Gin Lys Leu Ala Met Glu Thr Leu Glu 40 45 50 GAA TTA GAC TGG TGT TTA GAC CAG CTA GAG ACC ATA CAG ACC TAC CGG 485 Glu Leu Asp Trp Cys Leu Asp Gin Leu Glu Thr lie Gin Thr Tyr Arg 55 60 65 TCT GTC AGT GAG ATG GCT TCT AAC AAG TTC AAA AGA ATG CTG AAC CGG 533 Ser Val Ser Glu Met Ala Ser Asn Lys Phe Lys Arg Met Leu Asn Arg 70 75 80 GAG CTG ACA CAC CTC TCA GAG ATG AGC CGA TCA GGG AAC CAG GTG TCT 581 Glu Leu Thr His Leu Ser Glu Met Ser Arg Ser Gly Asn Gin Val Ser 85 90 95 100 GAA TAC ATT TCA AAT ACT TTC TTA GAC AAG CAG AAT GAT GTG GAG ATC 629 Glu Tyr lie Ser Asn Thr Phe Leu Asp Lys Gin Asn Asp Val Glu lie 105 110 115 CCA TCT CCT ACC CAG AAA GAC AGG GAG AAA AAG AAA AAG CAG CAG CTC 677 Pro Ser Pro Thr Gin Lys Asp Arg Glu Lys Lys Lys Lys Gin Gin Leu 120 125 130 ATG ACC CAG ATA AGT GGA GTG AAG AAA TTA ATG CAT AGT TCA AGC CTA 725 Met Thr Gin lie Ser Gly Val Lys Lys Leu Met His Ser Ser Ser Leu 135 140 145 AAC AAT ACA AGC ATC TCA CGC TTT GGA GTC AAC ACT GAA AAT GAA GAT 773 Asn Asn Thr Ser lie Ser Arg Phe Gly Val Asn Thr Glu Asn Glu Asp 150 155 160 CAC CTG GCC AAG GAG CTG GAA GAC CTG AAC AAA TGG GGT CTT AAC ATC 821 His Leu Ala Lys Glu Leu Glu Asp Leu Asn Lys Trp Gly Leu Asn lie 165 170 175 180 TTT AAT GTG GCT GGA TAT TCT CAC AAT AGA CCC CTA ACA TGC ATC ATG 869 Phe Asn Val Ala Gly Tyr Ser His Asn Arg Pro Leu Thr Cys lie Met 185 190 195 TAT GCT ATA TTC CAG GAA AGA GAC CTC CTA AAG ACA TTC AGA ATC TCA 917 Tyr Ala lie Phe Gin Glu Arg Asp Leu Leu Lys Thr Phe Arg lie Ser 200 205 210 TCT GAC ACA TTT ATA ACC TAC ATG ATG ACT TTA GAA GAC CAT TAC CAT 965 Ser Asp Thr Phe lie Thr Tyr Met Met Thr Leu Glu Asp His Tyr His 215 220 225 TCT GAC GTG GCA TAT CAC AAC AGC CTG CAC GCT GCT GAT GTA GCC CAG 1013 Ser Asp Val Ala Tyr His Asn Ser Leu His Ala Ala Asp Val Ala Gin 230 235 240 TCG ACC CAT GTT CTC CTT TCT ACA CCA GCA TTA GAC GCT GTC TTC ACA 1061 Ser Thr His Val Leu Leu Ser Thr Pro Ala Leu Asp Ala Val Phe Thr 245 250 255 260 GAT TTG GAG ATC CTG GCT GCC ATT TTT GCA GCT GCC ATC CAT GAC GTT 1109 Asp Leu Glu lie Leu Ala Ala lie Phe Ala Ala Ala lie His Asp Val 265 270 275 GAT CAT CCT GGA GTC TCC AAT CAG TTT CTC ATC AAC ACA AAT TCA GAA 1157 Asp His Pro Gly Val Ser Asn Gin Phe Leu lie Asn Thr Asn Ser Glu 280 285 290 CTT GCT TTG ATG TAT AAT GAT GAA TCT GTG TTG GAA AAT CAT CAC CTT 1205 Leu Ala Leu Met Tyr Asn Asp Glu Ser Val Leu Glu Asn His His Leu 295 300 305 GCT GTG GGT TTC AAA CTG CTG CAA GAA GAA CAC TGT GAC ATC TTC ATG 1253 Ala Val Gly Phe Lys Leu Leu Gin Glu Glu His Cys Asp lie Phe Met 310 315 320 AAT CTC ACC AAG AAG CAG CGT CAG ACA CTC AGG AAG ATG GTT ATT GAC 1301 Asn Leu Thr Lys Lys Gin Arg Gin Thr Leu Arg Lys Met Val lie Asp 325 330 335 340 ATG GTG TTA GCA ACT GAT ATG TCT AAA CAT ATG AGC CTG CTG GCA GAC 1349 Met Val Leu Ala Thr Asp Met Ser Lys His Met Ser Leu Leu Ala Asp 345 350 355 CTG AAG ACA ATG GTA GAA ACG AAG AAA GTT ACA AGT TCA GGC GTT CTT 1397 Leu Lys Thr Met Val Glu Thr Lys Lys Val Thr Ser Ser Gly Val Leu 360 365 370 CTC CTA GAC AAC TAT ACC GAT CGC ATT CAG GTC CTT CGC AAC ATG GTA 1445 Leu Leu Asp Asn Tyr Thr Asp Arg lie Gin Val Leu Arg Asn Met Val 375 380 385 CAC TGT GCA GAC CTG AGC AAC CCC ACC AAG TCC TTG GAA TTG TAT CGG 1493 His Cys Ala Asp Leu Ser Asn Pro Thr Lys Ser Leu Glu Leu Tyr Arg 390 395 400 CAA TGG ACA GAC CGC ATC ATG GAG GAA TTT TTC CAG CAG GGA GAC AAA 1541 Gin Trp Thr Asp Arg lie Met Glu Glu Phe Phe Gin Gin Gly Asp Lys 405 410 415 420 GAG CGG GAG AGG GGA ATG GAA ATT AGC CCA ATG TGT GAT AAA CAC ACA 1589 Glu Arg Glu Arg Gly Met Glu lie Ser Pro Met Cys Asp Lys His Thr 425 430 435 GCT TCT GTG GAA AAA TCC CAG GTT GGT TTC ATC GAC TAC ATT GTC CAT 1637 Ala Ser Val Glu Lys Ser Gin Val Gly Phe lie Asp Tyr lie Val His 440 445 450 CCA TTG TGG GAG ACA TGG GCA GAT TTG GTA CAG CCT GAT GCT CAG GAC 1685 Pro Leu Trp Glu Thr Trp Ala Asp Leu Val Gin Pro Asp Ala Gin Asp 455 460 465 ATT CTC GAT ACC TTA GAA GAT AAC AGG AAC TGG TAT CAG AGC ATG ATA 1733 lie Leu Asp Thr Leu Glu Asp Asn Arg Asn Trp Tyr Gin Ser Met lie 470 475 480 CCT CAA AGT CCC TCA CCA CCA CTG GAC GAG CAG AAC AGG GAC TGC CAG 1781 Pro Gin Ser Pro Ser Pro Pro Leu Asp Glu Gin Asn Arg Asp Cys Gin 485 490 495 500 GGT CTG ATG GAG AAG TTT CAG TTT GAA CTG ACT CTC GAT GAG GAA GAT 1829 Gly Leu Met Glu Lys Phe Gin Phe Glu Leu Thr Leu Asp Glu Glu Asp 505 510 515 TCT GAA GGA CCT GAG AAG GAG GGA GAG GGA CAC AGC TAT TTC AGC AGC 1877 Ser Glu Gly Pro Glu Lys Glu Gly Glu Gly His Ser Tyr Phe Ser Ser 520 525 530 ACA AAG ACG CTT TGT GTG ATT GAT CCA GAA AAC AGA GAT TCC CTG GGA 1925 Thr Lys Thr Leu Cys Val lie Asp Pro Glu Asn Arg Asp Ser Leu Gly 535 540 545 GAG ACT GAC ATA GAC ATT GCA ACA GAA GAC AAG TCC CCC GTG GAT ACA 1973 Glu Thr Asp lie Asp lie Ala Thr Glu Asp Lys Ser Pro Val Asp Thr 550 555 560 TAATCCCCCT CTCCCTGTGG AGATGAACAT TC ATCCTTG ATGAGCATGC CAGCTATGTG 2033 GTAGGGCCAG CCCACCATGG GGGCCAAGAC CTGCACAGGA CAAGGGCCAC CTGGCTTTCA 2093 GTTACTTGAG TTTGGAGTCA GAAAGCAAGA CCAGGAAGCA AATAGCAGCT CAGGAAATCC 2153 CACGGTTGAC TTGCCTTGAT GGCAAGCTTG GTGGAGAGGG CTGAAGCTGT TGCTGGGGGC 2213 CGATTCTGAT CAAGACACAT GGCTTGAAAA TGGAAGACAC AAAACTGAGA GATCATTCTG 2273 CACTAAGTTT CGGGAACTTA TCCCCGACAG TGACTGAACT CACTGACTAA TAACTTCATT 2333 TATGAATCTT CTCACTTGTC CCTTTGTCTG CCAACCTGTG TGCCTTTTTT GTAAAACATT 2393 TTCATGTCTT TAAAATGCCT GTTGAATACC TGGAGTTTAG TATCAACTTC TACACAGATA 2453 AGCTTTCAAA GTTGACAAAC TTTTTTGACT CTTTCTGGAA AAGGGAAAGA AAATAGTCTT 2513 CCTTCTTTCT TGGGCAATAT CCTTCACTTT ACTACAGTTA CTTTTGCAAA CAGACAGAAA 2573 GGATACACTT CTAACCACAT TTTACTTCCT TCCCCTGTTG TCCAGTCCAA CTCCACAGTC 2633 ACTCTTAAAA CTTCTCTCTG TTTGCCTGCC TCCAACAGTA CTTTTAACTT TTTGCTGTAA 2693 ACAGAATAAA ATTGAACAAA TTAGGGGGTA GAAAGGAGCA GTGGTGTCGT TCACCGTGAG 2753 AGTCTGCATA GAACTCAGCA GTGTGCCCTG CTGTGTCTTG GACCCTGCCC CCCACAGGAG 2813 TTGTACAGTC CCTGGCCCTG CTCCCTACCT CCTCTCTTCA CCCCGTTAGG CTGTTTTCAA 2873 TGTAATGCTG CCGTCCTTCT CTTGCACTGC CTTCTGCGCT AACACCTCCA TTCCTGTTTA 2933 TAACCGTGTA TTTATTACTT AATGTATATA ATGTAATGTT TTGTAAGTTA TTAATTTATA 2993 TATCTAACAT TGCCTGCCAA TGGTGGTGTT AAATTTGTGT AGAAAACTCT GCCTAAGAGT 3053 TACGACTTTT TCTTGTAATG TTTTGTATTG TGTATTATAT AACCCAAACG TCACTTAGTA 3113 GAGACATATG GCCCCCTTGG CAGAGAGGAC AGGGGTGGGC TTTTGTTCAA AGGGTCTGCC 3173 CTTTCCCTGC CTGAGTTGCT ACTTCTGCAC AACCCCTTTA TGAACCAGTT TTGGAAACAA 3233 TATTCTACAC ATTAGATACT AAATGGTTTA TACTGAGCTT TTACTTTTGT ATAGCTTGAT 3293 AGGGGCAGGG GGCAATGGAT GTAGTTTTTA CCCAGGTTCT ATCCAAATCT ATGTGGGCAT 3353 GAGTTGGGTT ATAACTGGAT CCT CTATCA TTGTGGCTTT GGTTCAAAAG GAAACACTAC 3413 ATTTGCTCAC AGATGATTCT TCTGAATGCT CCCGAACTAC TGACTTTGAA GAGGTAGCCT 3473 CCTGCCTGCC ATTAAGCAGG AATGTCATGT TCCAGTTCAT TACAAAAGAA AACAATAAAA 3533 CAATGTGAAT TTTTATAATA AAATGTGAAC TGATGTAGCA AATTACGCAA ATGTGAAGCC 3593 TCTTCTGATA ACACTTGTTA GGCCTCTTAC TGATGTCAGT TTCAGTTTGT AAAATATGTT 3653 TCATGCTTTC AGTTCAGCAT TGTGACTCAG TAAATACAGA AAATGGCACA AATGTGCATG 3713 ACCAATGTAT GTCTATGAAC ACTGCATTGT TTCAGGTGGA CATTTTATCG ATTTTCAAAT 3773 GTTTCTCACA ATGTATGTTA TAGTGTTATT ATTATATATT GTGTTCAAAT GCATTCTAAA 3833 GAGACTTTTA TATGAGGTGA ATAAAGAAAA GCATAATTAA AAAAAAAAAA AAAAAAA 3890 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 564 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: Met Lys Glu His Gly Gly Thr Phe Ser Ser Thr Gly lie Ser Gly Gly 1 5 10 15 Ser Gly Asp Ser Ala Met Asp Ser Leu Gin Pro Leu Gin Pro Asn Tyr 20 25 30 Met Pro Val Cys Leu Phe Ala Glu Glu Ser Tyr Gin Lys Leu Ala Met 35 40 45 Glu Thr Leu Glu Glu Leu Asp Trp Cys Leu Asp Gin Leu Glu Thr lie 50 55 60 Gin Thr Tyr Arg Ser Val Ser Glu Met Ala Ser Asn Lys Phe Lys Arg 65 70 75 80 Met Leu Asn Arg Glu Leu Thr His Leu Ser Glu Met Ser Arg Ser Gly 85 90 95 Asn Gin Val Ser Glu Tyr lie Ser Asn Thr Phe Leu Asp Lys Gin Asn 100 105 110 Asp Val Glu lie Pro Ser Pro Thr Gin Lys Asp Arg Glu Lys Lys Lys 115 120 125 Lys Gin Gin Leu Met Thr Gin lie Ser Gly Val Lys Lys Leu Met His 130 135 140 Ser Ser Ser Leu Asn Asn Thr Ser lie Ser Arg Phe Gly Val Asn Thr 145 150 155 160 Glu Asn Glu Asp His Leu Ala Lys Glu Leu Glu Asp Leu Asn Lys Trp 165 170 175 Gly Leu Asn lie Phe Asn Val Ala Gly Tyr Ser His Asn Arg Pro Leu 180 185 190 Thr Cys lie Met Tyr Ala lie Phe Gin Glu Arg Asp Leu Leu Lys Thr 195 200 205 Phe Arg lie Ser Ser Asp Thr Phe lie Thr Tyr Met Met Thr Leu Glu 210 215 220 Asp His Tyr His Ser Asp Val Ala Tyr His Asn Ser Leu His Ala Ala 225 230 235 240 Asp Val Ala Gin Ser Thr His Val Leu Leu Ser Thr Pro Ala Leu Asp 245 250 255 Ala Val Phe Thr Asp Leu Glu lie Leu Ala Ala lie Phe Ala Ala Ala 260 265 270 lie His Asp Val Asp His Pro Gly Val Ser Asn Gin Phe Leu lie Asn 275 280 285 Thr Asn Ser Glu Leu Ala Leu Met Tyr Asn Asp Glu Ser Val Leu Glu 290 295 300 Asn His His Leu Ala Val Gly Phe Lys Leu Leu Gin Glu Glu His Cys 305 310 315 320 Asp lie Phe Met Asn Leu Thr Lys Lys Gin Arg Gin Thr Leu Arg Lys 325 330 335 Met Val lie Asp Met Val Leu Ala Thr Asp Met Ser Lys His Met Ser 340 345 350 Leu Leu Ala Asp Leu Lys Thr Met Val Glu Thr Lys Lys Val Thr Ser 355 360 365 Ser Gly Val Leu Leu Leu Asp Asn Tyr Thr Asp Arg lie Gin Val Leu 370 375 380 Arg Asn Met Val His Cys Ala Asp Leu Ser Asn Pro Thr Lys Ser Leu 385 390 395 400 Glu Leu Tyr Arg Gin Trp Thr Asp Arg lie Met Glu Glu Phe Phe Gin 405 410 415 Gin Gly Asp Lys Glu Arg Glu Arg Gly Met Glu lie Ser Pro Met Cys 420 425 430 Asp Lys His Thr Ala Ser Val Glu Lys Ser Gin Val Gly Phe lie Asp 435 440 445 Tyr lie Val His Pro Leu Trp Glu Thr Trp Ala Asp Leu Val Gin Pro 45.0 455 460 Asp Ala Gin Asp lie Leu Asp Thr Leu Glu Asp Asn Arg Asn Trp Tyr 465 470 475 480 Gin Ser Met lie Pro Gin Ser Pro Ser Pro Pro Leu Asp Glu Gin Asn 485 490 495 Arg Asp Cys Gin Gly Leu Met Glu Lys Phe Gin Phe Glu Leu Thr Leu 500 505 510 Asp Glu Glu Asp Ser Glu Gly Pro Glu Lys Glu Gly Glu Gly His Ser 515 520 525 Tyr Phe Ser Ser Thr Lys Thr Leu Cys Val lie Asp Pro Glu Asn Arg 530 535 540 Asp Ser Leu Gly Glu Thr Asp lie Asp lie Ala Thr Glu Asp Lys Ser 545 550 555 560 Pro Val Asp Thr (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 40 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (F) TISSUE TYPE: brain (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..40 (D) OTHER INFORMATION: /function= "5 1 PCR probe" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: GGGGCTCGAG GAGGGACACA CGTATTTCAG CAGCACAAAG 40 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 40 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (B) STRAIN: Homo sapiens (F) TISSUE TYPE: brain (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..40 (D) OTHER INFORMATION: /function= "3* PCR probe" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: CTCACTTGAG TGACTGATTA TTGAAGTAAA GAGCTCGGGG 40 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 42 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo Sapiens (F) TISSUE TYPE: brain (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..42 (D) OTHER INFORMATION: /function= "5' PCR probe" (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: GGGGGCTCGA GAATGAAGGA GCACGGGGGC ACCTTCAGTA GC 42 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 30 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (F) TISSUE TYPE: Brain (ix) FEATURE: (A) NAME/KEY: misc_feature (B) LOCATION: 1..30 (D) OTHER INFORMATION: /function= π 3' PCR probe" ( i) SEQUENCE DESCRIPTION: SEQ ID NO:6: GACGGTAAAA ACGTCGACGG TAGGTACTGC 30

Claims: HAT TS CT-ATMED TS- 1. An isolated nucleic acid molecule encoding a human PDE IVJJ. 2. The molecule according to Claim 1 wherein said nucleic acid is DNA. 3. The molecule according to Claim 2 having substantially the same sequence as Seq. ID. No. 1. 4. A human PDE IVJJ substantially free of other proteins of human origin.. 5. The protein according to Claim 4 having substantially the same amino acid sequence as Seq. ID. No. 2. 6. A vector comprising the nucleic acid of Claim 1. 7. The vector according to Claim 6 which is a plasmid. 8. The plasmid according to Claim 7 which is a cloning plasmid. 9. The plasmid according to Claim 7 which is an expression plasmid. 10. The plasmid of Claim 9 which is identified as pl38NB/hb-PDEl. 11. A recombinant host cell comprising the vector of Claim 6. 12. The host cell according to Claim 11 which is a prokaryotic cell. 13. The host cell according to Claim 11 which is an eukaryotic cell. 14. The eukaryotic host cell according to Claim 13 which is a yeast. 15. A method for identifying ligands capable of binding to a PDE IVB enzyme comprising: contacting a PDE IVg enzyme with a plurality of candidate ligands labeled with an analytically detectable reagent under conditions sufficient for ligand binding and identifying those ligand candidates capable of enzyme binding by detecting the presence of a labeled ligand/enzyme complex. 16 The method according to Claim IS wherein the ligand is labeled with radioactivity. 17. A method of screening compounds to identify those compounds which bind to a human PDE IVg enzyme comprising contacting the enzyme with (a) a plurality of drug candidates in the presence of (b) an analytically detectable ligand known to bind to the enzyme, under conditions that permit binding of (a) and (b) to the enzyme, and identifying those candidate compounds capable of enhancing or inhibiting the binding or interaction of the known ligand with the enzyme. 18. The method according to Claim 17 wherein the known labeled ligand is selected from the group consisting of rolipram and cAMP. 19. The method according to Claim 18 wherein the label is a radioactive label. 20. The method according to Claim 19 wherein the known labeled ligand is 3-H-c AMP and the interaction detected is the catalytic conversion of the ligand. 21. A biological screening assay for the detection of PDE IVg selective ligands comprising: (a) providing a PDE deficient host cell that exhibit a specific growth arrest phenotype associated with elevated cAMP levels; (b) transforming or transfecting said host cell with the plasmid of Claim 9 and culturing the resultant recombinant host cell under conditions sufficient for the expression of PDE IVg enzyme and sufficient to generate a growth arrest response should the expressed PDE IVβ enzyme be inhibited; (c) contacting the recombinant host cell with a plurality of candidate compounds and (d) identifying those compounds which are capable of inhibiting the enzyme and thereby unmasking the growth arrest phenotype. 22. The method according to Claim 21 wherein said host cell is a yeast cell and said plasmid is pl38NB/hb-PDEl. 23. The method according to Claim 22 wherein said specific growth arrest phenotype is selected from the group consisting of sensitivity to nitrogen starvation, heat shock sensitivity and inability to grow on suboptimal carbon sources. 24. A pharmacuetical composition comprising a compound identified by the method of Claim 21 and a pharmaceutically acceptable carrier. 25. An antisense oligonucleotide having sequence capable of binding specifically with any sequence of an mRNA molecule which encodes the human PDE IVQ having the amino acid sequence of Seq. ID No 2 so as to prevent the translation thereof. 26. An antibody directed to the human PDE IVg of Claim 5. 27. The antibody according to Claim 26 which is a monoclonal antibody. 28. A fusion protein comprising a PDE IVg domain and an cell surface localizing domain. 29. A method of screening compounds to identify those compounds which bind to a human PDE rVg enzyme comprising contacting recombinant host cells expressing on the surface thereof the fusion protein of Claim 29 with a plurality of drug candidates, under conditions to permit binding to the PDE IVQ domain, and identifying those candidate drugs capable of enhancing or inhibiting the catalytic activity of the enzyme.

Inventors: LIVI GEORGE P, MCLAUGHLIN MEGAN M, TORPHY THEODORE J, LIVI, GEORGE, P., MCLAUGHLIN, MEGAN, M., TORPHY, THEODORE, J.
Applicants: LIVI GEORGE P, MCLAUGHLIN MEGAN M, SMITHKLINE BEECHAM CORP, TORPHY THEODORE J, LIVI, GEORGE, P., MCLAUGHLIN, MEGAN, M., SMITHKLINE BEECHAM CORPORATION, TORPHY, THEODORE, J.
Application year: 1994
Main classification: A61K31
IPC codes: C07H21, C07K14, C07K16, C07K19, C08L101, C12N1, C12N9, C12N15, C12P21, C12Q1, C12R1, G01N33