Peptides modified by the phosphine group for marking with 99mTC and 186-188Re or paramagnetic agents

Abstract: The peptides described are modified with the phosphine group and are useful for marking with 99m Tc and 186-188 Re or paramagnetic agents for use in diagnosis and radiotherapy; in particular chelating compounds having the co-ordinating sets PN 2 X, where X is -S, -O, COO-, PN 3 , P 2 N 3 and P 2 N 2 are described.

Description: The present invention relates to the use of molecules containing phosphine groups for labelling with 99m Tc , 186-188 Re or paramagnetic agents. The labelling of protein or peptide molecules with 99m Tc and 186-188 Re is the future challenge in the field of radiopharmaceuticals both for diagnosis and for radiotherapy. Quite a number of results relating to the coupling to protein molecules of so-called "chelating agents" which enable the radionuclide to remain firmly bonded thereto are already reported in the literature. Moreover, in recent years, chelating agents which contain small peptides (for example MAG 3 ) have been studied, that is, it has been established that, in the co-ordinating (chelating) set around the technetium or rhenium, amide groups or other groups belonging to amino-acids such as, for example, -SH, -COOH, -NH 2 , OH, etc., can contribute to the stabilization of the metal-chelating agent complex. The study of new tracers for magnetic resonance is also of very great interest. The principle for the design of these new molecules is again that of possessing a useful biological tropism. The difference lies in the tracer which, for magnetic resonance, has to have a highly paramagnetic element. The discovery of these molecules would allow this technique to make a qualitative jump from topology to functional diagnostics. Conference Technetium and Rhenium in Chemistry and Nuclear Medicine 3, Cortina International, 1989, Verona, Italy, pages 433-444, Edwards D.S. et al., discloses N 2 S 2 diaminedithiol ligands for technetium and rhenium and particularly a 99m Tc radiopharmaceutical, Neurolite™, based on the ligand N,N'-1,2-ethylenediylbis-L-cysteine, diethylester, which is reported to show promise as a marker of regional cerebral blood flow. J.Chem.Soc.Dalton Trans., vol.19, 1993, pages 2901-2908, Refosco Fiorenzo et al., discloses bidentate ligands with a phosphine-carboxylic acid donor set which form technetium complexes of the type TcL 3 , where L is the ligand. Nucl.Med.Biol., vol.14, n.5, 1987, pages 503-510, Neves Maria et al., discloses neutral technetium (II)- 99m complexes of the general formula tr-[ 99m Tc II D 2 X 2 ] 0 , where D represents a chelating ditertiary phosphine or arsine ligand and X represents a halide or pseudohalide ligand. WO93/08839 relates to multifunctional ligands for potential use in the design of therapeutic or diagnostic radiopharmaceutical imaging agents. EP-A-0 527 056 relates to oligopeptides which are disclosed for in vivo tumour imaging and therapy. As a result of the present invention, it has been found that the insertion of the phosphine group in peptides permits the production of molecules which are useful as chelating agents for 99m Tc, 186-188 Re or paramagnetic agents, these molecules using, as co-ordinating sets, the phosphine group and adjacent functional groups belonging to the peptide in question. The subject of the invention is therefore peptide molecules modified with the phosphine group as defined by the structural formulae given below and their use as chelating agents for technetium, rhenium and paramagnetic agents. In particular, the molecules of the invention conform to the following general structural formula : in which R 1 and R 2 are selected independently from the group consisting of: H, linear or branched C 1 -C 5 alkyl, linear or branched substituted C 1 -C 5 alkyl, OH, C 1 -C 5 alkoxy, phenoxy, and substituted phenyl groups, and in which A is alkylene carbonyl or alkylene amino, in which the alkylene has from 1 to 4 carbon atoms and may be linear or branched, and in which, when A is alkylene carbonyl, B is: in which R 3 is a group selected from the radicals constituting the lateral chain of a natural amino-acid; R 4 is selected from the group consisting of: H, linear or branched C 1 -C 4 alkyl (preferably methyl, ethyl, isopropyl and 2-methylpropyl) groups, -CH 2 -C 6 H 5 , -(CH 2 ) r NH 2 where r is an integer from 1 to 4, -(CH 2 ) p -CONH 2 , where p is an integer from 1 to 3, -(CH 2 ) n XR 5 where n is 1 or 2 and X is selected from -O - , -s - , and -COO - , and R 5 is H or a protecting group; Z is OH, OMe, NH 2 or is the same as R 4 , or Z is a peptide with biological properties useful for diagnosis, radiotherapy, or magnetic resonance, bonded to the preceding carbon atom by means of its N-terminal group; and in which, when A is alkylene amino, B is selected from the group consisting of: and where R 1 , R 2 , R 3 , R 4 , n and Z have the meanings given above and R 6 has the same meaning as R 3 given above, or has the meaning of Z given above, and Y is H or where R 1 , R 2 and n have the meanings given above. When R 1 and R 2 in formula (I) are substituted alkyl groups, the substituents are preferably hydroxyl, ether (preferably C 1 - C 5 alkoxy),ester (preferably C 1 - C 5 COO-), amide (preferably CO-NH 2 ), ketone (preferably -CO-(C 1 -C 4 ) alkyl), aldehyde (preferably -COH) or nitrile groups. When R 1 and R 2 in formula (I) are substituted phenyl, the substituents are preferably selected from hydroxyl, ketone, nitrile, ether (-O-C 1 -C 5 ), ester and amide groups as defined above. When X is -S - , the protecting groups R 5 at X are preferably selected from C 1 -C 5 alkyl, phenyl, and substituted benzoyl groups, for example, such as terbutyl, methoxymethyl, 2-tetrahydropyranyl, benzyl, 3,4-dimethylbenzyl, p-methoxybenzyl, p-hydroxy-o-acetoxybenzyl, benzylthiomethyl, p-nitrobenzyl, diphenylmethyl, bis(4-methoxyphenyl)methyl, phenyl, 2,4-dinitrophenyl, acetylbenzoyl, thiobenzoyl, butoxycarbonyl, benzyloxycarbonyl, and N-methoxymethyl groups; when X is COO - , R 5 is preferably a linear or branched C 1 -C 5 alkyl group or a C 1 -C 5 alkyl group substituted with C 1 -C 5 alkylester or arylester groups; when X is O - , R 5 is preferably a C 1 -C 5 alkyl group, an aryl group or a C 1 -C 5 alkylcarbonyl or arylcarbonyl group such as, for example, an acetyl, phenyl, benzyl, terbutyl, methyl, benzyloxycarbonyl, BOC, 1-benzyloxycarbonylamino-2,2,2-trifluoroacetyl, or o-trialkylsilyl group. When Z is a molecule with biological properties useful for diagnosis, radiotherapy or magnetic resonance, it is selected, for example, from avidin, biotin, low-density lipoprotein (LDL), chemotactic peptides for targeting inflammatory sites and infection processes, peptides for targeting tumours (for example, somatostatin and derivatives thereof), peptides for targeting blood clots and atherosclerotic plaques, cytotoxic agents, monoclonal antibodies and fragments thereof. Groups of compounds preferred within the scope of the invention comprise compounds having the following general formulae: in which n is 1 or 2 and the other symbols have the meanings given above. From the point of view of the co-ordination around the metal, formulae (II) and (III) are considered as containing the co-ordinating set PN 2 X and are constituted by the phosphine group and a dipeptide condensed by means of a peptide or amide bond. Compounds of the general formula (IV) and (V) are also included within the scope of the invention: in which Z is amino or a peptide useful for diagnosis, radiotherapy or NMR, connected by means of its N-terminal group (NH-peptide), and in which the coordinating set is PN 3 . Another general formula within the scope of the invention is that in which the phosphine group is bonded to the carboxyl group of aspartic acid (VI): in which Z is NH 2 or NH-peptide. Formula (VI) may be used to bond a second phosphine group -PR 1 R 2 to the -NH 2 terminal and thus to form the co-ordinating set P 2 N 3 (general structural formula VII): Molecules which use the co-ordinating set P 2 N 2 , obtained by bonding two phosphine groups to a single amino-acid having the general formula (VIII) are also included: The modification of the peptides with a phosphine group can be carried out with the use of the normal "coupling" reaction (see, in the literature, M. Bodansky, A. Bodansky; "The Practice of Peptide Synthesis", Springer Verlag, New York, 1984 - bibliographical reference (1)), for example, by means of reactions which can be shown schematically as follows: or in which Su is the succinimide radical. For the reaction conditions see bibliographical reference (1). The modification can also be achieved by other reactions such as: These molecules are synthesized in order to label protein or peptide substances with radioactive or paramagnetic metals and thus to be able to follow their behaviour in vivo and thereby to establish a clinical diagnosis or to carry out radiotherapy. In particular, the molecules according to the invention are useful for complexing 99m Tc and 186-188 Re, preferably with an oxidation state of +5. Lower oxidation states can only be achieved in some cases. Paramagnetic agents which can be complexed preferably comprise gadolinium or iron. Complexes comprising Re, Tc or the aforementioned paramagnetic agents, as well as the use of these complexes for diagnosis, radiotherapy and magnetic resonance applications are intended to fall within the scope of the invention. Example 1 Synthesis of Ph 2 P(CH 2 ) 2 CONH-gly-cys-(Bzl)COOMe. BOC-gly, Hcys(Bzl)COOMe.HCl and Ph 2 P(CH 2 ) 2 COOH were obtained commercially. A. Preparation of BOC-gly-OSu (bibl. ref. (1)): 1.72 g (10 mmoles) of BOC-gly were dissolved n 170 ml of methylene chloride; 1.28 g (12 mmoles) of N-hydroxysuccinimide were added thereto. After stirring for five minutes in a bath at 0°C , 2.5 g (12 mmoles) of dicyclohexylcarbo-diimide were added. The reaction mixture was left for twelve hours at 4°C with stirring. The precipitate of decyclohexyl urea was filtered off and the solution was brought to dryness on a rotavapor. The pure product was obtained by recrystallization from isopropanol (yield 75%). B: Synthesis of Hgly.cys(Bzl)COOMe (bibl. ref.(1)): 3.7 g (14 mmoles) of Hcys(Bzl)COOMe.HCl were placed in anhydrous methylene chloride (50 ml) with Et 3 N (2.1 ml, 15 mmoles) and stirred for ten minutes. A solution of BOCglyCOOSu (3.9 g, 14 mm) in 100 ml of anhydrous methylene chloride was then added to this mixture. The reaction was followed by TLC. Upon completion of the reaction (several hours) the impurities were eliminated by successive extractions with 1) 10 -1 M HCl, 2) 5% NaHCO 3 , 3) H 2 O. A yellowish oil was obtained by evaporation (yield 94%). The BOC was deprotected with trifluoroacetic acid. The product was recovered by evaporation of the acid on a rotavapor and then with an oil pump. The oily product was transformed into the hydrochloride by alcoholic solution saturated with HCl and was precipitated with ethyl ether (yield (93%). C. Synthesis of Ph 2 P(CH 2 ) 2 COOSu: The carboxyl group of the phosphine was activated with an activated ester in an inert atmosphere (argon) to prevent oxidation of the phosphine group to phosphine oxide. 2.58 g (10 mmoles) of Ph 2 P(CH 2 ) 2 COOH were placed in 100 ml of anhydrous dioxane and degassed with argon. 1.38 g (12 mmoles) of N-hydroxysuccinimide were added thereto. After a few minutes with stirring, 2.5 g (12 mmoles of dicyclohexylcarbo-diimide (DCC) were added to the mixture. The white precipitate of dicyclohexyl urea was filtered off and the solution was brought to a small volume by a rotavapor. A white powder was obtained and could be washed with small quantities of anhydrous methanol under argon (yield 65%). D. Synthesis of Ph 2 P(CH 2 ) 2 CONH-gly-cys(Bzl)COOMe (L): coupling reaction between Hgly-cys(Bzl)COOMe and Ph 2 P(CH 2 ) 2 COOSu. Hgly-cys(Bzl)COOMe.HCl (1.9 g, 6 mmoles) was dissolved in anhydrous dioxane and degassed with argon. 0.91 ml (6.5 mmoles) of Et 3 N were added to the mixture. The white triethylamine hydrochloride was eliminated by filtration. 2.14 g of Ph 2 P(CH 2 ) 2 COOSu, equal to 6 mmoles, were added to the degassed solution and the mixture was left with stirring. The reaction was checked by TLC. Upon completion of the reaction, the solvent was eliminated by evaporation. The oil obtained was dissolved in ethyl acetate and the impurities were eliminated by extraction with water degassed with argon. After dehydration, the dioxane was eliminated by evaporation. The product was recrystallized with ethyl acetate-petroleum ether (yield 76%). The presence of about 5% of phosphine oxide was confirmed by means of the 31 P NMR spectrum. Formula of the product obtained: The co-ordinating set in this case is PN 2 S. Example of application Tests for coordination and for labelling with 99m Tc were carried out on this ligand. The co-ordination tests carried out with rhenium led to the identification of two species which contained the ligand intact and which were assumed to contain the molecule in the equatorial plane relative to the ReO 3+ group. Reaction: Structure: This shows that the thioether group also permits co-ordination. The thiol group can be deprotected with a consequent variation of the co-ordinating capacity of the ligand. The first results of the 99m Tc labelling tests of the same molecule led to a yield of up to 85% with the use of the pair of reducing agents Sn(tartrate) and NaBH 4 . These conditions indicate that NaBH 4 can deprotect the thiol group of cysteine and lead to good labelling. The labelling yield will improve if a protector group suitable for deprotection in the labelling conditions is used on the cysteine thiol. Other results indicate that very high labelling yields can be achieved even in mild conditions (only SnCl 2 as the reducing agent); this indicates that a stable technetium complex can be formed even if the ligand contains a thioether or if there is no deprotection of the cysteine thiol. From the results obtained, moreover, the possibility of co-ordinating the carboxyl group in place of the thiol group cannot be excluded; in this case, the complex becomes: Following the synthesis method described in Example 1, it is possible to prepare the molecules given by way of example in Table 1, which conform to the general formula: in which n is 1 or 2 and Z is COOH, COOMe or a peptide having useful biological properties, as defined above and in which R 1 , R 2 , R 3 and R 5 have the meanings given in Table 1.

Claims: Chelating compounds having the following general structural formula: in which R 1 and R 2 are selected independently from the group consisting of: H, linear or branched C 1 -C 5 alkyl, linear or branched substituted C 1 -C 5 alkyl, OH, C 1 -C 5 alkoxy, phenoxy, and substituted phenyl groups, and in which A is alkylene carbonyl or alkylene amino in which the alkylene has from 1 to 4 carbon atoms and may be linear or branched, and in which, when A is alkylene carbonyl, B is: in which R 3 is a group selected from the radicals constituting the lateral chain of a natural amino-acid; R 4 is selected from the group consisting of: H, linear or branched C 1 -C 4 alkyl (preferably methyl, ethyl, isopropyl and 2-methylpropyl) groups, -CH 2 -C 6 H 5 , -(CH 2 ) r NH 2 where r is an integer from 1 to 4, -(CH 2 ) p -CONH 2 where p is an integer from 1 to 3, -(CH 2 ) n XR 5 where n is 1 or 2 and X is selected from -O - , -S - , and -COO - , and R 5 is H or a protecting group; Z is OH, OMe, NH 2 or is the same as R 4 , or Z is a peptide with biological properties useful for diagnosis, radiotherapy or magnetic resonance, bonded to the preceding carbon atom by means of its N-terminal group; and in which, when A is alkylene amino, B is selected from the group consisting of: and where R 1 , R 2 , R 3 , R 4 , n and Z have the meanings given above and R 6 has the same meaning as R 3 given above, or has the meaning of Z given above, and Y is H or where R 1 , R 2 and n have the meanings given above. Compounds according to Claim 1 in which when R 1 and R 2 in formula (I) are substituted alkyl, the substituents are hydroxyl, ether (preferably C 1 - C 5 alkoxy), ester (preferably C 1 - C 5 COO-), amide, ketone, aldehyde or nitrile groups. Compounds according to Claim 1 in which, when R 1 and R 2 in formula (I) are substituted phenyl, the substituents are selected from hydroxyl, ketone, nitrile, ether (-O-C 1 -C 5 ), ester and amide groups. Compounds according to any one of Claims 1 to 3, in which, when X is -S - , the protecting groups R 5 at X are selected from C 1 -C 5 alkyl, phenyl, and substituted benzoyl groups. Compounds according to Claim 4 in which the protecting groups are selected from the group consisting of terbutyl, methoxymethyl, 2-tetrahydropyranyl, benzyl, 3,4-dimethylbenzyl, p-methoxybenzyl, p-hydroxy-o-acetoxybenzyl, benzylthiomethyl, p-nitrobenzyl, diphenylmethyl, bis(4-methoxyphenyl)methyl, phenyl, 2,4-dinitrophenyl, acetylbenzoyl, thiobenzoyl, butoxycarbonyl, benzyloxycarbonyl, and N-methoxymethyl groups. Compounds according to any one of Claims 1 to 3 in which, when X is COO - , R 5 is a linear or branched C 1 -C 5 alkyl group or a C 1 -C 5 alkyl group substituted with C 1 -C 5 alkylester or arylester groups. Compounds according to any one of Claims 1 to 4, in which, when X is O - , R 5 is a C 1 -C 5 alkyl group, an aryl group or a C 1 -C 5 alkylcarbonyl or arylcarbonyl group. Compounds according to Claim 7, in which R 5 is selected from acetyl, phenyl, benzyl, terbutyl, methyl, benzyloxycarbonyl, BOC, 1-benzyloxycarbonylamino-2,2,2-trifluoroacetyl, and o-trialkylsilyl groups. Compounds according to Claim 1 in which, when Z is a molecule with biological properties useful for diagnosis, radiotherapy or magnetic resonance, it is selected, for example, from avidin, biotin, low-density lipoprotein (LDL), chemotactic peptides for displaying inflammatory sites and infection processes, peptides for displaying tumours, peptides for displaying blood clots and atherosclerotic plaques, cytotoxic agents, monoclonal antibodies and fragments thereof. Compounds according to any one of Claims 1 to 9, having a co-ordinating set PH 2 X according to the following general formulae: in which n is 1 or 2 and the other symbols have the meanings given above. Compounds according to any one of Claims 1 to 3, having a co-ordinating set PN 3 of the general formula (IV) and (V): in which Z is amino or a peptide useful for diagnosis, radiotherapy or NMR, connected by means of its N-terminal group (NH-peptide). Compounds according to any one of Claims 1 to 3 in which the phosphine group is bonded to the carboxyl group of aspartic acid of the general formula (VI): in which Z is NH 2 or NH-peptide. Compounds according to any one of Claims 1 to 3, having a co-ordinating set P 2 N 3 with the general structural formula VII: Compounds according to any one of Claims 1 to 3 which use the co-ordinating set P 2 N 2 , obtained by bonding two phosphine groups to a single amino-acid, having the general formula (VIII): Use of peptide compounds modified with phosphine according to any one of Claims 1 to 14 as a chelating agent for 99m Tc, 186-188 Re or a paramagnetic agent. Use according to Claim 15, in which the paramagnetic agent is selected from gadolinium and iron. Complexes of 99m Tc, 186-188 Re or a paramagnetic agent with a chelating compound according to any one of Claims 1 to 14. A diagnostic kit for radiotherapy or magnetic resonance including an effective quantity of a chelating compound according to any one of Claims 1 to 14.

Inventors: LUNGHI FABIO, MAZZI ULDERICO, LUNGHI,FABIO, MAZZI,ULDERICO
Applicants: NYCOMED AMERSHAM SORIN S R L, NYCOMED AMERSHAM SORIN S.R.L.
Application year: 1994
Main classification: C07K5
IPC codes: A61K49, C07K5, A61K51, A61K38, C07F13