DIAMINOCYCLOBUTENE-3,4-DIONES AS SMOOTH MUSCLE RELAXANTS

Abstract: The compounds of formula (I) wherein R1 and R2 are, independently, hydrogen, straight or branched chain alkyl or mono- or bi-cyclic alkyl; A is an N-heterocycle which may be substituted by alkyl, perfluoroalkyl, alkoxy, perfluoroalkoxy, amino, alkylamino, dialkylamino, alkylsulfonamido, alkylcarboxamido, nitro, cyano or carboxyl; or, A is a substituted phenyl group containing one or two substituents selected from cyano, nitro, alkyl, perfluoroalkyl, alkoxy, perfluoroalkoxy, amino, alkylamino, dialkylamino, sulfamyl, alkylsulfonamido, arylcarboxamido, alkylsulfonyl, perfluoroalkylsulfonyl, arylsulfonyl, chloro, bromo, fluoro, iodo, 1-imidazolyl or carboxyl; or a pharmaceutically acceptable salt thereof, are smooth muscle relaxants.

Description: DIAMIN0CYCL0BUTENE-3, 4-DIONES AS SMOOTH MUSCLE RELAXANTS Background of Invention The present invention relates to novel 1 , 2-diamino derivatives of cyclobutene 3-4-diones having pharmacological activity, to a process for their preparation, to pharmaceutical compositions containing them, and to their use in the treatment of disorders associated with smooth muscle contraction; via potassium channel modulation. Such disorders include, but are not limited to: urinary incontinence, hypertension, asthma, premature labor, irritable bowel syndrome, congestive heart failure, angina, and cerebral vascular disease. Stemp et al. disclose a class of amino substituted cyclobutenedione derivatives of chromans described as having blood pressure lowering activity and bronchodilatory activity in EP-426379-A2. Several series of l-amino-2- phenylalkylamino-cyclobutene-3,4-diones are reported as H-2 receptor antagonists by Algieri et al. in US Patent 4,390,701. Several related l-amino-2-phenoxyalkylamino derivatives are disclosed by Nohara et al. in US Patent 4,673,747. The syntheses of variously substituted l,2-diamino-cyclobutene-3,4-diones are described in the following publications: Tietze et al., Chem Ber. 1991, 124, 1215; Tietze et al., Bioconjugate Chem. 1991, 2, 148; Ehrhardt et al., Chem. Ber. 1977, 110, 2506, and Neuse et al., Liebigs Ann. Chem. 1973, 619. For example, Neuse et al. discloses l-phenylamino-2-dimethylamino-cyclobut-l-ene-3,4-dione. The compounds of the present invention differ from the Neuse et al. compound in that they are substituted on the phenylamino group and they are useful as smooth muscle relaxants. Description of The Invention Accordingly, the present invention discloses compounds represented by the formula (I): N N H R. (I) wherein: Rl and R2 are, independent from each other, hydrogen, Cl-lO straight chain alkyl, Cl-lO branched alkyl , or C3-IO cyclic or bicyclic alkyl; A is selected from the group consisting of: wherein: R3 is h ogen, Ci- alkyl, Ci-6 perfluoroalkyl, Ci- alkoxy, perfluoroalkoxy, amino, Ci-6 alkylamino, Ci-12 dialkylamino, Ci- alkylsulfonamido, alkyl- carboxamido containing 2 to 7 carbon atoms, nitro, cyano, carboxyl; or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, Ci-6 alkyl, Ci- perfluoroalkyl, Ci-6 alkoxy, Ci-6 perfluoroalkoxy, amino, Ci- alkylamino, C2- 12 dialkylamino, sulfamyl, Ci-6 alkylsulfonamido, C6-12 arylsulfonamido, alkylcarboxamido containing 2 to 7 carbon atoms, arylcarboxamido containing 7 to 13 carbon atoms, C1-6 alkylsulfonyl, Cl-6 perfluoroalkylsulfonyl, Co- 12 arylsulfonyl, chloro, bromo, fluoro, iodo, 1-imidazolyl, carboxyl or hydrogen, with the proviso that R4 and R5 cannot be hydrogen simultaneously; or a pharmaceutically acceptable salt thereof. A preferred aspect of this invention includes compounds of formula (I) wherein: Rl and R2 are as stated above; A is selected from the following: wherein: R3 is as stated above; or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, chloro, bromo, fluoro, iodo, 1- imidazolyl,. carboxyl or hydrogen, with the proviso that R4 and R5 cannot be hydrogen simultaneously; or a pharmaceutically acceptable salt thereof. The most preferred aspect of this invention includes compounds of formula (I) wherein: Rl and R2 are as stated above; A is selected from the following: wherein: R3 is as stated above; or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, chloro, bromo, fluoro, iodo, 1- imidazolyl, carboxyl or hydrogen, with the proviso that R4 and R5 cannot both be hydrogen simultaneously; or a pharmaceutically acceptable salt thereof. It is understood that the definition of the compounds of formula (I), when Ri, R2, R3, R4. or R5 contain asymmetric carbons, encompass all possible stereoisomers and mixtures thereof which possess the activity discussed below. In particular, it encompasses racemic modifications and any optical isomers which possess the indicated activity. Optical isomers may be obtained in pure form by standard separation techniques. The compounds of this invention, throughout this specification, are equivalently name as 3,4-diones or 1,2-diones. The pharmaceutically acceptable salts of the basic compounds of this invention are those derived from such organic and inorganic acids as: lactic, citric, acetic, tartaric, succinic, maleic, malonic, hydrochloric, hydrobromic, phosphoric, nitric, sulfuric, methanesulfonic, and similarly known acceptable acids. Where R3, R4, or R5 is a carboxyl group, salts of the compounds of this invention may be formed with bases such as alkali metals (Na, K, Li) or the alkaline earth metals (Ca or Mg). The present invention also provides a process for the preparation of a compound of formula (I). More particularly, the compounds of formula (I) may be prepared by reacting a compound of formula (Ila): wherein X is a leaving group, for example, methoxy, ethoxy, isopropoxy, halogen or a similar leaving group and Ai is A, as defined hereinbefore or a group of atoms convertible thereto, with a compound of formula (IV): R a1 HN. R a2 (IV) wherein R j and R-^ are R j and R2, respectively, as defined hereinbefore or a group of atoms convertible thereto and, where appropriate, converting A 1 into A or converting R 1 into R j or converting R a 2 into R2 and, where desired, converting a compound having formula (I) into a pharmaceutically acceptable salt thereof or converting a salt of a compound having formula (I) into a compound having formula (I). The compounds having formula (Ila) may be prepared by reaction of a compound having (II): where X is as defined above with a compound having the formula (III): A ι — NH 2 (m) wherein A j is as defined above. The reactions mentioned above may be carried out in a solvent such as methanol or ethanol at elevated temperatures. As mentioned previously, the compounds of formula (I) and their pharmaceutically acceptable salts have been found to relax smooth muscle. They are therefore useful in the treatment of disorders associated with smooth muscle contraction, disorders involving excessive smooth muscle contraction of the urinary tract (such as incontinence), or of the gastro-intestinal tract (such as irritable bowel syndrome), asthma, and hair loss. Furthermore, the compounds of formula (I) are active as potassium channel activators which render them useful for treatment of peripheral vascular disease, hypertension, congestive heart failure, stroke, anxiety, cerebral anoxia and other neurodegenerative disorders. The present invention accordingly provides a pharmaceutical composition which comprises a compound of this invention in combination or association with a pharmaceutically acceptable carrier. In particular, the present invention provides a pharmaceutical composition which comprises an effective amount of a compound of this invention and a pharmaceutically acceptable carrier. The compositions are preferably adapted for oral administration. However, they may be adapted for other modes of administration, for example, parenteral administration for patients suffering from heart failure. In order to obtain consistency of administration, it is preferred that a composition of the invention is in the form of a unit dose. Suitable unit dose forms include tablets, capsules and powders in sachets or vials. Such unit dose forms may contain from 0.1 to 100 mg of a compound of the invention and preferably from 2 to 50 mg. Still further preferred unit dosage forms contain 5 to 25 mg of a compound of the present invention. The compounds of the present invention can be administered orally at a dose range of about 0.01 to 100 mg/kg or preferably at a dose range of 0.1 to 10 mg/kg. Such compositions may be administered from 1 to 6 times a day, more usually from 1 to 4 times a day. The compositions of the invention may be formulated with conventional excipients, such as a filler, a disintegrating agent, a binder, a lubricant, a flavoring agent and the like. They are formulated in conventional manner, for example, in a manner similar to that used for known antihypertensive agents, diuretics and β- blocking agents. The present invention further provides a compound of the invention for use as an active therapeutic substance. Compounds of formula (I) are of particular use in the induction of smooth muscle relaxation. The present invention further provides a method of treating smooth muscle disorders in mammals including man, which comprises administering to the afflicted mammal an effective amount of a compound or a pharmaceutical composition of the invention. The following examples are presented to illustrate rather than limit the methods for production of representative compounds of the invention. Example 1 3-(Pyridin-4-vIamino)-4-(1.2.2-trimethyl-proDylaιnino)- cvclobut-3-ene- 1.2-dione Step 1) Preparation of 3-(pyridin-4-ylamino)-4-ethoxy-cyclobut-3-ene-l,2- dione To a solution of 3,4-diethoxy-3-cyclobutene-l,2-dione (5.00g, 29.4 mmol) in absolute ethanol (100 mL) was added a suspension of 4-aminopyndine {2.11 g, 29 A mmol) in ethanol (50 mL). The reaction mixture was refluxed for 4 hours then concentrated to give crude product. Chromatography (EtOAc) afforded 0.632 g (10%) of a white solid: mp 120-125 °C. Step 2) Preparation of 3-(pyridin-4-ylamino)-4-(l,2,2-trimethyl- propylamino)-cyclobut-3-ene- 1 ,2-dione To the above squarate (0.332 g, 1.52 mmol) in acetonitrile (30 mL) was added 2-amino-3,3-dimethylbutane (0.200 ml, 1.52 mmol). A precipitate formed while stirring overnight. The crude reaction mixture was vacuum filtered and the precipitate was dried in vacuo to yield 0.328 g (79%) of a pale yellow solid: mp 255- 257 °C; NMR (DMSO-d 6 ): δ 9.80 (s, 1H), 8.42 (dd, 2H), 7.72 (d, 1H), 7.45 (dd, 2H), 3.96-4.00 (m, 1H), 1.18 (s, 3H), 0.91 (s, 9H). IR (KBr): 3200, 1800, 1675, 1600 cm-1; MS (m/z) 274 (MH+). Elemental analysis for C15H19N3O2 Calc'd: C, 65.91; H, 7.01; N, 15.37 Found: C, 65.91; H, 6.96; N, 15.22 Example 2 (Exo)-3-(bicvclor2.2.11hept-2-ylamino ) -4- ( Dyridin-4-ylamino -cvclobut-3- ene-1.2-dione To the product of Example 1, Step 1 (0.332 g, 1.52 mmol) in acetonitrile (30 mL) was added (+) exo-2-aminonorbornane (0.180 mL, 1.52 mmol). A precipitate forms while stirring overnight. The reaction mixture was vacuum filtered and the precipitate was dried in vacuo to yield 0.350g (81%) of a pale yellow solid: mp 268- 270°C (dec); J H NMR (DMSO-d 6 ): δ 9.70 (s, IH), 8.40 (d, 2H), 7.74 (d, IH), 7.42 (d, 2H), 3.90-3.95 (m, IH), 2.23-2.31 (m, 2H), 1.78-1.84 (m, IH), 1.09-1.54 (m, 7H). IR (KBr): 3200, 1795, 1670, 1600 cm" 1 ; MS (m/z) 283 (M + ). Elemental analysis for C16H17N3O2 Calc'd: C, 67.83; H, 6.05; N, 14.83 Found: C, 67.48; H, 6.03; N, 14.66 Example 3 3- yridin-3-ylamino)-4-fl.2.2-trin.ethvI-Dropylaτnino.- cvclohut-3-ene- 1.2-dione Step 1) Preparation of 3-(pyridin-3-ylamino)-4-ethoxy-cyclobut-3-ene-l,2- dione To a solution of 3,4-diethoxy-3-cyclobutene-l,2-dione (5.00g, 29.4 mmol) in absolute ethanol (lOOmL) was added a suspension of 3-aminopyridine (2.77 'g, 29.4 mmol) in ethanol (50 mL). The mixture was heated at reflux for 18 hours, then concentrated. Chromatography (4:1 EtOAc/hexane) afforded 3.15 g (49%) of a white solid: mp 140-145 °C. Step 2) Preparation of 3-(pyridin-3-ylamino)-4-( 1 ,2,2-trimethyl- propylamino)-cyclobut-3-ene- 1 ,2-dione To the above squarate (0.328 g, 1.50 mmol) in acetonitrile (30 mL) was added 2-amino-3,3-dimethylbutane (0.200 mL, 1.52 mmol). A precipitate forms while stirring overnight. The crude reaction mixture was vacuum filtered and the precipitate was dried in vacuo to yield 0.27 g (66%) of a white solid: mp 243-245°C; *H NMR (DMSO-d 6 ): δ 9.67 (s, IH), 8.58 (d, 1H),7.98 (d, IH), 7.66 (d, IH), 7.38 (m, IH), 3.96-4.00 (m, IH), 1.18 (s, 3H), 0.95 (s, 9H) ; IR (KBr): 3200, 1800, 1665, 1600 cm- 1 . MS (m/z) 273 (M+). Elemental analysis for C15H19N3O2 Calc'd: C, 65.91; H, 7.01; N, 15.37 Found: C, 65.92; H, 6.95; N, 15.24 Example 4 3-f2-Methylethylamino.-4-(pyridin-3-ylaτnino.(cvclobut- 3-ene-1.2-dione To the product from Example 3, Step 1 (0.180 g, 0.825 mmol) in acetonitrile (100 mL) was added isopropylamine (20 mL, 235 mmol). The reaction was stirred at room temperature overnight, then concentrated. Trituration with ethylacetate/di- ethylether afforded 0.135 g (71%) of a white solid: mp 258-260 °C; Η NMR (DMSO-d 6 ): δ 9.62 (br s, IH), 8.55 (s, IH), 8.22 (d, IH), 7.94 (br d, IH), 7.73 (br s, IH), 7.36 (dd, IH), 4.20 (br m, IH), 1.25 (d, 6H). IR (KBr): 3200, 1800, 1660, 1610 cm-l; MS (m/z) 231(M + ). Elemental analysis for C12H13N3O2 Calc'd: C, 62.32; H, 5.66; N, 18.17 Found: C, 62.86; H, 5.79; N, 18.53 Example 5 3-Dimethylamino-4-(pyridin-3-ylamino.-cvclobut-3-ene-1.2-dione To the product from Example 3, Step 1 (0.330 g, 1.51 mmol) in acetonitrile (200 mL) was introduced to a stream of dimethylamine gas (15 minutes). The resultant solution was stirred overnight at room temperature, concentrated, and triturated with dichloromethane/diethylether to afford 0.278g (85%) of an white solid: mp 235-237 °C; *H NMR (DMSO-dό): δ 9.50 (s, IH), 8.46 (s, IH), 8.22 (d, IH), 7.60 (d, IH), 7.32 (dd, IH). IR (KBr): 1790, 1685, 1600 cπr*; MS (m z) 217 (M+). Elemental analysis for C11H11N3O2 Calc'd: C, 60.82; H, 5.10; N, 19.34 Found: C, 60.71; H, 5.07; N, 19.46 Example 6 3-Amino-4-(pyridιn-3-ylamino -cvclobut-3-ene-1.2-dione To the product from Example 3, Step 1 (0.330 g, 1.51 mmol) in acetonitrile (200 mL) was introduced to a stream of ammonia gas until solution became turbid. The resultant mixture was stirred overnight at room temperature. The precipitate was vacuum filtered and dried to afford 0.266g (93%) of a white solid: mp 297 °C(dec); *H NMR (DMSO-d 6 ): δ 8.56 (s, IH), 8.22 (d, IH), 7.92 (d, IH), 7.37 (m, IH). IR (KBr): 3200, 1800, 1670, 1625 cm" 1 ; MS (m/z) 189 (M+). Elemental analysis for C9H7N3O2 Calc'd: C, 57.14; H, 3.73; N, 22.21 Found: C, 57.18; H, 3.69; N, 22.10 Example 7 3-Tert-butylamino-4-(pyridin-3-ylamino)-cvcIobut-3-ene-1.2-dione The product from Example 3, Step 1 (2.60 g, 11.9 mmol) was dissolved in tert-butylamine (50 mL). The solution was refluxed for three hours, cooled, and concentrated in vacuo. Trituration with diethylether afforded 1.05 g (36%) of a white solid: mp 250-252 °C; *H NMR (DMSO-d 6 ): δ 8.57 (s, IH), 8.23 (d, IH), 7.96 (d, IH), 7.37 ( , IH) 1.43 (s,9H). IR (KBr): 1790, 1685, 1600 cm" 1 ; MS (m/z) 245 (M + ). Elemental analysis for C13H15N3O2 Calc'd: .C, 63.66; H, 6.16; N, 17.13 Found: C, 63.28; H, 6.22; N, 17.07 Example 8 3-Tert-butylamino-4-(isoquinolin-5-ylamino)-cvclobut- 3-ene-l,2- ione Step 1) Preparation of 3-(isoquinolin-5-ylamino)-4-ethoxy- cyclobut-3-ene- 1 ,2-dione To a solution of 3,4-diethoxy-3-cyclobutene-l,2-dione (5.00g, 29.4 mmol) in absolute ethanol (100 mL) was added a suspension of 5-aminoisoquinoline (4.24g, 29.4 mmol) in ethanol (50 mL). The mixture was heated to reflux overnight and then filtered to yield 2.30 g (29%) of solid: mp 182(dec) °C. Step 2) Preparation of 3-Tert-butylamino-4-(isoquinolin-5- ylamino)-cyclobut-3-ene-l,2-dione one-eighth hydrate The above squarate (0.300 g, 1.12 mmol) was dissolved in tert-butylamine (50 mL) and refluxed for three hours. The mixture was cooled, concentrated, and triturated with diethylether to afford 0.120 g (39%) of the title compound as a white solid, one-eighth hydrate: mp 268-270 °C(dec); J H NMR (DMSO-dό): δ 9.75 (s, IH), 9.35 (s, IH), 8.62 (d, IH), 8.19 (s, IH), 8.01 (d, IH), 7.88 (d, IH), 7.80 (d, IH), 7.68 (t, IH), 1.47 (s, 9H). IR (KBr): 3200, 1785, 1670, 1600 cm' 1 ; MS (m/z) 295 (M+). Elemental analysis for C17H17N3O2 • 0.125 (H2O) Calc'd: .C, 68.61; H, 5.84; N, 14.12 Found: C, 68.08; H, 5.78; N, 13.75 Example 9 3-Amino-4-(isoαuinolin-5-ylamino.-cvclobut-3-ene-1.2-dione A suspension of the the product from Example 8, Step 1 (0.190 g, 0.700 mmol) in ethanol (3.5 mL) was saturated with ammonia, capped, and heated to 45°C for three hours. The mixture was cooled, concentrated, and triturated with diethylether . Crude product was recrystallized from dimethylformamide/water to give 0.129 g (77%) of the title compound as a pale yellow solid, one-eighth hydrate: mp 215°C; Η NMR (DMSO-d 6 ): δ 9.87 (s, IH), 9.34 (s, IH), 8.60 (d, IH), 7.40- 8.60 (broad signal, NH2), 8.02 (d, IH), 7.86 (d, IH), 7.77 (d, IH), 7.68 (t, IH). IR (KBr): 3200, 1800, 1690, 1650 cm" 1 ; MS (m/z) 240(MH+). Elemental analysis for C13H9N3O2 • 0.125 (H2O) Calc'd: C, 64.66; H, 3.86; N, 17.40 Found: C, 64.10; H, 3.74; N, 16.99 Example 10 3-(Ouinolin-8-ylamino)-4-(1.2.2-trimethyl-propylamino - cvclobut-3-ene- 1.2-dione Step 1) Preparation of 3-(quinolin-8-ylamino)-4-ethoxy-cyclobut-3-ene- 1,2-dione To a solution of 8-aminoquinoline (1.00 g, 6.94 mmol) in ethanol (20 mL) was added 3,4-diethoxy-3-cyclobutene-l,2-dione (1.03 mL, 6.94 mmol) and the resulting mixture was heated to reflux for 24 hours. The mixture was cooled, diluted with ethanol, and filtered. The crude product was triturated with chloroform/hexanes, then purified by chromatography (EtOAc/hexane) to give 1.31 g (70%) of product: IH NMR (DMSO-d6) δ 9.75 (br m, 1 H), 8.86 (dd, 1 H), 8.26 (br m, IH), 8.20 (dd, IH), 7.57 (m, 2H), 7.53 (dd, IH), 4.95 (q, 2H), 1.59 (t, 3H). Step 2) Preparation of 3-(quinolin-8-ylamino)-4-(l ,2,2-trimethylpropylamino)- cyclobut-3-ene- 1 ,2-dione To the above squarate (0.300 g, 1.12 mmol) in ethanol (5 mL) was added 2- amino-3,3-dimethylbutane (0.18 mL, 1.34 mmol) and the resulting mixture was heated at 45° C overnight, diluted with hexanes, and filtered to give 0.294 g (81%) of a yellow solid: mp 244-245; H NMR (DMSO-d6) δ 10.45 (s, IH), 8.97 (dd, IH), 8.62 (d, IH), 8.41 (dd, IH), 8.29 (dd, IH), 7.6 (m, 3H), 4.10 (m, IH), 1.21 (d, 3H), 0.94 (s, 9H). IR (KBr): 3280, 2960, 1790, 1670 cm " 1 ; MS (m/z) 323 (MH+). Elemental analysis for C19H21N3O2 Calc'd: C, 70.56; H, 6.54; N, 12.99 Found: C, 70.38; H, 6.51; N, 12.94 Example 11 3-Methylamino-4-(quinoIin-8-ylamino)-cvclobut- 3-ene-1.2-dione To the product from Example 10, Step 1 (0.64 g, 2.38 mmol) was added ethanolic methylamine (8.03 M, 13 mL) and the mixture was stirred at 25 °C for 6 hours. Diethyl ether was added, and the resulting solid was filtered and washed with additional ether. Chromatography (EtOAc/hexanes) followed by trituration with dimethylsulfoxide/water afforded 0.398 g (66%) of product as a yellow solid: mp 236-237°C; l H NMR (DMSO-d6) δ 10.32 (s, IH), 8.95 (dd, IH), 8.63 (m, IH), 8.40 (dd, IH), 8.26 (d, IH), 7.68-7.54 (m, 3H), 3.27 (d, 3H). IR (KBr) 3200, 1795, 1610 cm-1; MS (m z) 253 (M+), 155 (100%). Elemental analysis for C14H11N3O2 Calc'd: C, 66.39; H, 4.38; N, 16.59 Found: C, 66.13; H, 4.43; N, 16.48 E a lg 12 3-Amino-4-(quinolin-8-ylamino -cvclohut-3-ene-1.2-dione Ammonia gas was bubbled through a slurry of the product from Example 10, Step 1 (0.640 g, 2.38 mmol) in ethanol (13 mL) at -35 °C. After 6 hours, the mixture was diluted with diethylether and filtered. Chromatography (EtOAc/hexanes) followed by trituration with dimethylsulfoxide/water afforded 0.342 g (60%) of product as a yellow solid, quarter hydrate: mp 263°C (dec); H NMR (DMSO-d6) δ 10.35 (s, IH), 8.95 (dd, IH), 8.45 (br s, 2H), 8.40 (dd, IH), 8.28 (dd, IH), 7.66-7.55 (m, 3H). IR (KBr) 3260, 1800 cm " l; MS (m/z) 239 (M+), 211, 183, 155, 129 (100%). Elemental analysis for C13H9N3O2 • 0.25 (H2O) Calc'd: C, 64.06; H, 3.72; N, 17.24 Found: C, 63.28; H, 3.85; N, 16.99 Example 13 3-(Ouinolin-3-ylamino.-4-(1.2.2-trimethyl-propylaπ_ino.-cvclobut- 3-ene-1.2-dione Step 1) Preparation of 3-(Quinolin-3-ylamino)-4-ethoxy-cyclobut-3-ene- 1,2-dione To a solution of 3-aminoquinoline (2.12 g, 14.69 mmol) in ethanol (60 mL) was added 3,4-diethoxy-3-cyclobutene-l,2-dione (2.50 g, 14.69 mmol) and the resulting mixture was heated to reflux for 24 hours. The mixture was cooled, filtered, and the product was washed with diethylether and dried in vacuo to give 3.14 g (80%) of yellow solid which was used without purification: ^H NMR (DMSO-d6) δ 11.14 (s, IH), 8.92 (dd, IH), 8.20 (dd, IH), 7.90 (m, 2H), 7.63 (m, 2H). Step 2) Preparation of 3-(Quinolin-3-ylamino)-4-( 1 ,2,2-trimethyl- propylamino)-cyclobut-3-ene-l,2-dione To the above squarate (0.30 g, 1.12 mmol) in ethanol (8 mL) was added 2- amino-3,3-dimethylbutane (0.18 mL, 1.34 mmol) and the resulting mixture was heated at 50° C overnight. The precipitate was filtered, washed with diethylether, and dried in vacuo to afford 0.30 g (83%) of product as a light tan solid: mp 242-246°C; 1H NMR (DMSO-d6) δ 9.93 (s, IH), 8.90 (d, IH), 8.38 (br s, IH), 7.97 (d, IH), 7.84 (dd, IH), 7.75-7.55 (m, 2H), 4.00 (m, IH), 1.20 (d, 3H), 0.94 (s, 9H). IR (KBr) 3160, 2960, 1795, 1650 cm-*; MS (m/z) 324 (MH+). Elemental analysis for C19H21N3O2 Calc'd: C, 70.57; H, 6.55; N, 12.99 Found: C, 70.97; H, 6.41; N, 13.04 Example 14 3-Tert-butylamino-4-(quinolin-3-ylamino)-cvclobut-3-ene-1.2-dione To the product from Example 13, Step 1 (0.30 g, 1.12 mmol) in ethanol (8 mL) was added tert-butylamine (0.35 mL, 3.36 mmol) and the resulting mixture was heated at 60° C overnight. The mixture was concentrated, and the residue was dissolved in ethylacetate. Addition of hexanes induced precipitation of product which was filtered and triturated with diethylether/hexanes. Filtration and drying afforded 0.28 g (85%) of the title compound as a yellow solid, three -quarter hydrate: mp 257- 260°C; iH NMR (DMSO-d6) d 10.00 (br d, IH), 8.9 (d, IH), 8.4 (m, IH), 8.02 (d, IH), 7.96 (br d, IH), 7.86 (dd, IH), 7.66-7.54 (m, 2H), 1.45 (s, 9H). IR (KBr) 3400, 3200, 2980, 1785, 1680 cπr*; MS (m/z) 296 (MH+). Elemental analysis for C17H17N3O2 • 0.75 (H2O) Calc'd: C, 66.11; H, 6.04; N, 13.61 Found: C, 66.08; H, 5.86; N, 13.36 Examnle 15 3-(Ouinolin-3-ylamino ) -4- ( l.l-dimethyl-propylaminoVcvclobut- 3-ene-l-2-dione To the product from Example 13, Step 1 (0.30 g, 1.12 mmol) in ethanol (7 mL) was added tert-amylamine (0.39 mL, 3.35 mmol). After 48 hours of stirring at 45°C, an additional aliquot of amine (0.39 mL) was added. After 3 hours, the mixture was concentrated and the residue was triturated with hexane/diethylether to give 0.328 g (95%) of product as an off white solid, three-quarter hydrate: mp 234- 236°C; 1 H NMR (DMSO-d6) δ 10.04 (br s, IH), 8.9 (d, IH), 8.4 (d, IH), 7.95 (d, IH), 7.86, (m, 2H), 7.6 (m, 2H), 1.78 (q, 2H), 1.40 (s, 6H), 0.89 (t, 3H). IR (KBr) 3370, 3240, 2960, 1785, 1680 cm- 1 ; MS (m/z) 309 (M+). Elemental analysis for C18H19N3O2 • 0.75 (H2O) Calc'd: C, 66.96; H, 6.40; N, 13.01 Found: C, 67.11; H, 6.26; N, 13.05 Example 16 3-(6-Methoxy-αuinolin-8-ylamino.-4-(1.2.2-trimethyl-propylamino - cvclobut-3-ene- 1.2-dione Step 1) Preparation of 3-(6-Methoxy-quinolin-8-ylamino)-4— ethoxy- cyclobut-3-ene-l,2-dione In a procedure identical to Example 10, Step 1, 8-amino-6-methoxyquinoline (2.00 g, 11.48 mmol) was reacted with 3,4-diethoxy-3-cyclobutene-l,2-dione (1.70 mL, 11.48 mmol) to give 0.99 g (29%) of product: l B NMR (DMSO-dό) δ 10.21 (s, IH), 8.75 (dd, IH), 8.29 (dd, IH), 7.61 ( , 2H), 7.17 (d, IH), 4.76 (q, 2H), 3.90 (s, 3H), 1.40 (t, 3H). Step 2) Preparation of 3-(6-Methoxy-quinolin-8-ylamino)-4~(l,2,2-trimethyl- propylamino)-cyclobut-3-ene-l,2-dione In a procedure identical to Example 10, Step 2, the above squarate (0.25 g, 0.839 mmol) and 2-amino-3,3-dimethylbutane (0.13 mL, 1.01 mmol) were reacted to give 0.21 g (71%) of title compound as an off-white solid: mp 243-245°C; !H NMR (DMSO-d6) δ 10.43 (br s, IH), 8.78 (dd, IH), 8.64 (d, IH), 8.28 (dd, IH), 8.13 (d, IH), 7.56 (dd, IH), 7.02 (d, IH), 4.11 (m, IH), 3.88 (s, 3H), 1.20 (d, 3H), 0.94 (s, 9H). IR (KBr) 3400, 3220, 2950, 1780 cm-1; MS (m/z) 353 (M+). Elemental analysis for C20H23 3O3 Calc'd: C, 67.96; H, 6.55; N, 11.88 Found: C, 67.72; H, 6.56; N, 11.74 Example 17 3-(6-Methoxy-quinolin-8-ylamino)-4-methylaminQ- cvclobut-3-ene- 2-dione The product from Example 16, Step 1 (0.25 g, 0.839 mmol) was added to 8 N ethanolic methylamine (7 mL). The resulting mixture was stirred for 4 hours and filtered. The solid was recrystallized from dimethylsulfoxide/water to give 0.12 g (50%) of product as a yellow solid: mp >275°C; 1H NMR (DMSO-d6) δ 10.29 ( br s, IH), 8.75 (dd, IH), 8.68 (br q, IH), 8.27 (dd, IH), 8.10 (d, IH), 7.55 (m, IH), 7.00 (d, IH), 3.88 (s, 3H), 3.26 (d, 3H). IR (KBr) 3240, 1795 cm-1; MS (rn / z ) 2 83 (M+), 185 (100%). Elemental analysis for C15H13N3O3 Calc'd: C, 63.59; H, 4.62; N, 14.83 Found: C, 63.30; H, 4.58; N, 14.70 Example . S 3-Tert-butylamino-4-(qu * ' .-6-ylamino .-cvclobut- To a solution of 3,4-diethoxy-3-cyclobatene-l,2-dione (5.00g, 29.4 mmol) in absolute ethanol (100 mL) was added a suspension of 6-aminoquinoline (4.24 g, 29.4 mmol) in ethanol (50 mL). The mixture was heated at reflux for 18 hours, cooled, and vacuum filtered to afford 6.64 g (84%) of crude product (mp: 185-187, dec) which was used without further purification. An aliquot (3.00 g, 11.2 mmol) was dissolved in tert-butylamine (50 mL). The solution was refluxed for three hours, cooled, and concentrated. The residue was recrystallized twice from ethanol then triturated with diethylether to give 1.05g (31%) of the title compound as a pale yellow solid, one-quarter hydrate: mp 234-236 °C; Η NMR (DMSO-dό): δ 9.92 (s, IH), 8.76 (d, IH), 8.24 (d, IH), 8.01 (d, IH), 7.98 (s, IH), 7.97 (d, IH), 7.88 (d, IH), 7.48 (m, IH), 1.45 (s, 9H). IR (KBr): 1780, 1665, 1610 cπr ; MS (m/z) 295 (M+). Elemental analysis for C17H17N3O2 • 0.25 (H2O) Calc'd: C, 68.10; H, 5.88; N, 14.01 Found: C, 67.63; H, 5.92; N, 13.96 Example 19 3-Tert-butylamino-4-(lH-inda/ol-6-ylamino)-cvclobut- 3-ene-1.2-dione To a solution of 3,4-diethoxy-3-cyclobutene-l,2-dione (4.51g, 26.5 mmol) in absolute ethanol (100 mL) was added a suspension of 6-aminoindazole (3.53g, 29.4 mmol) in ethanol (50 mL). The mixture was refluxed for 18 hours, cooled, and vacuum filtered to afford 3.60 g (53%) of crude product which was used without further purification. An aliquot (2.00 g, 7.77 mmol) was dissolved in tert-butylamine (50 mL) and the resulting mixture was refluxed for three hours, cooled, and concentrated. The crude product was recrystallized from ethanol/water to give 0.45 g (19%) of the title compound as a white solid, dihydrate: mp 183-185 °C; *H NMR (DMSO-d 6 ): δ 13.00 (s, IH), 9.74 (s, IH), 7.97 (s, IH), 7.92 (s, IH), 7.88 (s, IH), 7.70 (d, IH), 7.05 (d, IH), 1.44 (s, 9H). IR (KBr): 3200, 1790, 1655, 1600 cπr*; MS (m/z) 284 (M + ). Elemental analysis for C15H16N4O2 • 2 (H2O) Calc'd: C, 56.24; H, 6.29; N, 17.49 Found: C, 56.06; H, 6.29; N, 17.43 Example 20 3-(Isoαuinolin-5-ylamino -4-methylamino-cvclobut- 3-ene-1.2-dione To a solution of 3,4-diethoxy-3-cyclobutene-l,2-dione (5.00g, 29.4 mmol) in absolute ethanol (100 mL) was added a suspension of 5-aminoisoquinoline (4.24 g, 29.4 mmol) in ethanol (50 mL). The mixture was heated at reflux for 18 hours, cooled, and vacuum filtered to afford 2.30 g (29%) of crude product (mp: 182, dec) which was used without further purification. To an aliquot (0.335 g, 1.25 mmol) of the squarate in ethanol (1.5 mL) was added 33% methylamine in ethanol (1.4 mL). The mixture was heated to 40°C for 15 minutes, cooled, and concentrated. Trituration with diethylether afforded crude product which was crystallized from a minimal amount of methanol to give 0.149 g (47%) of an pale yellow solid: mp 245- 250 °C (dec); *H NMR (DMSO-d 6 ): δ 9.79 (s, IH), 9.33 (s, IH), 8.60 (d, IH), 7.99 (d, IH), 7.86 (d, IH), 7.76 (br d, IH), 7.66 (t, IH), 7.76 (br s, IH). IR (KBr): 3200, 1800, 1680, 1605 cm" 1 ; MS (m/z) 253 (M + ). Elemental analysis for C14H 11 N3O2 Calc'd: C, 66.40; H, 4.38; N, 16.59 Found: C, 66.06; H, 4.35; N, 16.32 Example 21 4-r3.4-Dioxo-2-(1.2.2-trimethyl-propylamino.- cvclobut-l-enylaminol-benzonitrile Step 1) Preparation of 4-(3,4-Dioxo-2-ethoxy-cyclobut-l-enylamino)- benzonitrile 4-Aminobenzonitrile (3.47g, 29.4 mmol) was added to a solution of 3,4- diethoxy-3-cyclobutene-l,2-dione (5.00g, 29.4 mmol) in absolute ethanol (100 mL). The mixture was heated at reflux overnight. The mixture was cooled, and the resulting yellow precipitate was collected by vacuum filtration. Yield: 2.60 g (37%): mp 218-222 °C. Step 2) Preparation of 4-[3,4-Dioxo-2-(l,2,2-trimethyl-propylamino)- cyclobut- 1 -enylamino]-benzonitrile To the above squarate (1.00 g, 4.13 mmol) in acetonitrile (250 mL) was added 2-amino-3,3-dimethylbutane (0.600 mL, 4.48 mmol). A precipitate forms while stirring overnight. The crude reaction mixture is vacuum filtered. The solid is triturated with diethylether to afford 0.620 g (50%) of product as a yellow solid: mp 241-243°C ; *H NMR (DMSO-d 6 ): δ 9.89 (s, IH), 7.78 (d, 2H), 7.72 (d, IH), 7.60 (d, 2H), 3.95-4.00 (m, IH), 1.18 (d, 3H), 0.91 (s, 9H). IR (KBr): 3200, 1790, 1660, 1600 cm- 1 ; MS (m/z) 297 (M + ). Elemental analysis for C17H19N3O2 Calc'd: C, 68.67; H, 6.44; N, 14.13 Found: C, 68.63; H, 6.15; N, 14.30 Example 22 (Exo.-4-r2-(bicvclor2.2.11hept-2-ylamino)-3.4-dioxo-cvclobut- 1-enylaminol-benzonitrile To the product of Example 21, Step 1 (0.368 g, 1.52 mmol) in acetonitrile (30 mL) was added (+)exo-2-aminonorbornane (0.180 mL, 1.52 mmol). A precipitate forms while stirring overnight. The crude reaction mixture was vacuum filtered and triturated with diethylether to afford 0.370 g (79%) of a yellow solid: mp 288-290°C ; NMR (DMSO-d 6 ): δ 9.79 (s, IH), 7.78 (d, 2H), 7.76 (m, IH), 7.58 (d, 2H), 3.90-3.98 (m, IH), 2.22-2.34 (m, 2H), 1.76-1.86 (m, IH), 1.08-1.56 (m, 7H). IR (KBr): 2220, 1790, 1650, 1600 cm- 1 ; MS (m/z) 307 (M + ). Elemental analysis for C18H17N3O2 Calc'd: C, 70.34; H, 5.57; N, 13.67 Found: C, 70.17; H, 5.50; N, 13.96 Example 23 4-r3.4-Dioxo-2-(1.2.2-trimethvl-propvlamino.-cvclohιιt- l-enylaminol-benzenesulfonamide To a slurry of sulfanilamide (1.72 g, 10.0 mmol) in ethanol (10 mL) was added 3,4-diethoxy-3-cyclobutene-l,2-dione (2.43 g, 14.3 mmol). The reaction was heated at reflux overnight, cooled, and a yellow precipitate was collected by vacuum filtration. Yield: 2.60 g (98%): mp 210-212 °C. To the above squarate (0.750 g, 2.84 mmol) in ethanol (10 mL) was added 2- amino-3,3-dimethylbutane (0.380 mL, 2.84 mmol). The mixture was heated to reflux overnight then concentrated. The residue was dissolved in acetone/ethylacetate (1:1) and filtered through a plug of silica gel to afford 0.200 g (20%) of the title compound as a white solid, hemi-hydrate: mp 233-235°C ; ! H NMR (DMSO-dβ): δ 9.87 (s, IH), 7.79 (d, 2H), 7.75 (d, IH), 7.58 (d, 2H), 7.25 (s, 2H), 4.00 (m, IH), 1.18 (d, 3H), 0.92 (s, 9H). IR (KBr): 1790, 1670, 1600 cm" 1 ; MS (m/z) 351 (M+). Elemental analysis for C16H21N3O4S • 1/2 H2O Calc'd: C, 53.32; H, 6.15; N, 11.66 Found: C, 52.89; H, 5.81; N, 11.42 Example 24 ( - .-rR -4-r3.4-Dioxo-2-π.2.2-trimethyl-propylamino.-cvclohut-l- enylaminol-3-ethyl-benzonitrile Step 1) Preparation of 4-(2-ethoxy-3,4-dioxo-cyclobut- 1 -enylamino)- 3-ethylbenzonitrile 4-Amino-3-ethylbenzonitrile (0.86 g, 5.88 mmol) and 3,4-diethoxy-3- cyclobutene-l,2-dione (1.0 g, 5.88 mmol) in acetonitrile (2 mL) was heated in an oil bath at 110°C for 21 h. 3,4-diethoxy-3-cyclobutene-l,2-dione (0.5 g, 2.9 mmol) was added to the reaction mixture and the temperature of the oil bath increased to 150°C. After 48 h the reaction mixture was cooled to room temperature, then diluted with ethyl acetate and filtered. The filtrate was concentrated and the resulting solid was taken up in ethyl acetate (5 mL) and sonicated. Filtration gave 0.54 g (34%) of a light brown solid: l H NMR (DMSO-d 6 ): δ 10.56 (br s, IH), 7.70 (br s overlapping a doublet at δ 7.69, 2H), 7.30 (d, IH), 4.71 (q, 2H), 2.74 (q, 2H), 1.37 (t, 3H), 1.15 (t, 3H). Step 2) (+)-(R)-4-[3,4-Dioxo-2-(l,2,2-trimethyl-propylamino)-cyclobut-l- enylamino]-3-ethyl-benzonitrile 4-(2-ethoxy-3,4-dioxo-cyclobut-l-enylamino)-3-ethyl-benzonitrile (1.26 g, 4.66 mmol) and a solution of (R)-l,2,2-trimethylpropylamine (9.3 mmol) in ethanol (58 mL) were stirred at room temperature for 24 h. The resulting yellow solution was concentrated to a yellow oil, which was dissolved in acetonitrile (20 mL) and stirred at room temperature. The resulting yellow solid was filtered and rinsed with ethyl acetate to yield 0.79 g (52%) of an off-white solid: mp 235-237°C; [α]* = + 68.20° (DMSO, c 0.0072); l H NMR (DMSO-d 6 ): δ 8.04 (br s, IH), 8.04 (d, IH), 7.69- 7.57 (m, 3H), 4.05 (m, IH), 2.71 (q, 2H), 1.27-1.16 (overlapping doublet and triplet, 6H), 0.91 (s, 9H). IR (KBr): 3278, 2959, 2222, 1793, 1674, 1598, 1576, 1522 cm" 1 ; MS (m/z) 325 (M + ). Elemental analysis for C19H23N3O2 Calc'd: C, 70.13; H, 7.12; N, 12.91 Found: C, 69.82; H, 7.17; N, 12.99 Example 25 (+ .-(R -4-r3,4-Dioxo-2-(1.2.2-trimethyl-propylamino -cvclobut-l- enylaminol-3-methoxy-benzonitrile Step 1) Preparation of 4-(2-ethoxy-3,4-dioxo-cyclobut-l-enylamino)- 3-methoxybenzonitrile To a cooled solution of 3-methoxybenzonitrile (10 g, 75 mmol) in acetic anhydride (150 ml) was added 70% nitric acid (60 mL) at a rate that kept the reaction mixture below 50°C. After 1 h the reaction mixture was poured onto ice (400 cc) and extracted with ethyl acetate. The ethyl acetate layer was washed with water, and dried (Na2SO4). Concentration under reduced pressure and chromatography with ethyl acetate/hexanes gave 2.69 g (20%) of 3-methoxy-4-nitrobenzonitrile: *H NMR (DMSO-dβ): δ 8.04 (d, IH), 7.93 (d, IH), 7.61 (dd, IH), 3.97 (s, 3H). 3-Methoxy-4- nitrobenzonitrile (2.6 g, 14.6 mmol) was suspended in ethanol(100 mL) and added to a mixture of 5% platinum on carbon (250 mg) in ethanol (150 mL). The reaction mixture was stirred under a hydrogen gas atmosphere. After 24 h the reaction was filtered, concentrated, and chromatographed (ethyl acetate/hexanes, 25/75) to yield 1.3 g (60%) of 4-amino-3-methoxybenzonitrile: l NMR (DMSO-dό): δ 7.1 (m, 2H), 6.65 (d, IH), 5.77 (br s, 2H), 3.79 (s, 3H). 4-Amino-3-methoxybenzonitrile (1.3 g, 8.77 mmol) and 3,4-diethoxy-3-cyclobutene-l,2-dione (1.5 g, 8.82 mmol) in ethanol (50 mL) was heated in an oil bath at 110-115°C for 3 days. The reaction mixture was filtered hot, and the filtrate was reduced to half its volume. Filtration gave 0.58 g (24%) of a yellow solid: mp 159-161°C; l H NMR (DMSO-d 6 ): δ 10.42 (br s, IH), 7.54 (d, IH), 7.44 (dd, IH), 7.39 (d, IH), 4.70 (q, 2H), 3.86 (s, 3H), 1.36 (t, 3H). IR (KBr): 3413, 3026, 2650, 2576, 2439, 1719, 1630, 1548 cm" 1 ; MS (m/z) 272 (M+). Elemental analysis for C 1 4H12N2O4 • 0.2 H2O Calc'd: C, 60.95; H, 4.53; N, 10.15 Found: C, 60.96; H, 4.32; N, 10.23 Step 2) (+)-(R)-4-[3,4-Dioxo-2-(l,2,2-trimethyl-propylamino)- cyclobut- 1 -enylamino] -3-methoxy-benzonitrile 4-(2-ethoxy-3,4-dioxo-cyclobut-l-enylamino)-3-methoxy-benzonitrile (0.58 g, 2.1 mmol) and a solution of (R)-l,2,2-trimethylpropylamine (4.2 mmol) in ethanol (21 mL) were stirred at room temperature for 24 h. The reaction mixture was filtered, and the filtrate was concentrated to a foam, which was dissolved in acetonitrile (10 mL). Upon standing at room temperature fluffy pale yellow needles formed. This was filtered and rinsed sparingly with acetonitrile to yield 0.46 g (67%) of a pale yellow needles: mp 273-277°C (dec); [α] ? = + 62.86° (DMSO, c 0.010); X H NMR (DMSO-d 6 ): δ 9.49 (br s, IH), 8.31 (d, IH), 7.99 (d, IH), 7.50 (d, IH), 7.43 (dd, IH), 4.03 (m, IH), 3.97 (s, 3H), 1.18 (d, 3H), 0.90 (s, 9H). MS (m/z) 327 (M+). Elemental analysis for C18H21N3O3 Calc'd: C, 66.04; H, 6.47; N, 12.84 Found: C, 66.02; H, 6.39; N, 12.61 The smooth muscle relaxing activity of the compounds of this invention was established in accordance with standard pharmaceutically accepted test procedures in representative compounds as follows: Sprague-Dawley rats (150-200 g) are rendered unconscious by CO2 asphyxiation and then euthanized by cervical dislocation. The bladder is removed into warm (37 deg.C) physiological salt solution (PSS) of the following composition (mM): NaCl, 118.4; KC1, 4.7; CaCl 2 , 2.5; MgS0 4 , 4.7; H 2 0, 1.2; NaHC0 3 , 24.9; KH 2 PO 4 , 1-2; glucose, 11.1; EDTA, 0.023; gassed with 95% O2; 2/5% CO 2 ; pH 7.4. The bladder is opened and then cut into strips 1-2 mm in width and 7-10 mm in length. The strips are subsequently suspended in a 10 mL tissue bath under an initial resting tension of 1.5 g. The strips are held in place by two surgical clips one of which is attached to a fixed hook while the other is attached to an isometric force transducer. The preparations, which usually exhibit small spontaneous contractions, are allowed to recover for a period of 1 hour prior to a challenge with 0.1 uM carbachol. The carbachol is then washed out and the tissue allowed to relax to its resting level of activity. Following one further 30 minute period of recovery, an additional 15 mM KC1 are introduced into the tissue bath. This increase in KC1 concentration results in a large increase in the amplitude of spontaneous contractions (and initiation of contractions in previously quiescent strips) superimposed upon a small increase in basal tone. Following stabilization of this enhanced level of contractile activity, incremental increases in the concentration of test compound or vehicle are introduced into the tissue bath. Contractile activity is measured for each compound or vehicle concentration during the last minute of a 30 minute challenge. The isometric force developed by the bladder strips is measured using a concentration required to elicit 50% inhibition of pre-drug contractile activity (IC50 concentration) is calculated from this concentration-response curve. The maximum percentage inhibition of contractile activity evoked by a test compound is also recorded for concentrations of test compound less than or equal to 30 μM. The results of this study are shown in Table I. Table I Inhibition of Contractions in Isolated Rat Bladder Strips Compound no. of IC^ Inhibition of Force animals (%) at (x) μM Example 1 6 1.36 μM - Example 2 2 15.85 μM - Example 3 3 1.95 μM - Example 6 2 - 31% (30 μM) Example 7 2 2.8 μM - Example 8 4 1.84 μM - Example 9 4 - 43% (30 μM) Example 10 4 8.0 μM - Example 14 4 - 51% (30 μM) Example 19 4 6.34 μM - Example 21 2 0.52 μM - Example 24 2 0.021 μM Example 25 2 0.29 μM Hence, the compounds of this invention have a pronounced effect on smooth muscle contractility and are useful in the treatment of urinary incontinence, irritable bladder and bowel disease, asthma, hypertension, stroke, and similar diseases as mentioned above, which are amenable to treatment with potassium channel activating compounds by administration, orally, parenterally, or by aspiration to a patient in need thereof.

Claims: What is claimed is; -1- A compound of the formula: wherein: Rl and R2 are, independent from each other, hydrogen, Cl-lO straight chain alkyl, Cl-lO branched alkyl , or C3-10 cyclic or bicyclic alkyl; A is is selected from the group consisting of: wherein: R3 is hydrogen, Ci-6 alkyl, C 1 λ -6 perfluoroalkyl, C 1-6 alkoxy, Ci-6 perfluoroalkoxy, amino, Ci-6 alkylamino, C2-12 dialkylamino, Cl-6 alkylsulfonamido, alkylcarboxamido containing 2 to 7 carbon atoms, nitro, cyano, carboxyl; or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, Ci- alkyl, Ci-6 perfluoroalkyl, Cl-6 alkoxy, Ci-6 perfluoroalkoxy, amino, Cl-6 alkylamino, C2- 12 dialkylamino, sulfamyl, Cl-6 alkylsulfonamido, C6-12 arylsulfonamido, alkylcarboxamido containing 2 to 7 carbon atoms, arylcarboxamido containing 7 to 13 carbon atoms, Cl-6 alkylsulfonyl, Cl-6 perfluoroalkylsulfonyl, C6-12 arylsulfonyl, chloro, bromo, fluoro, iodo, 1- imidazolyl, carboxyl or hydrogen, with the proviso that R4 and R5 cannot be hydrogen simultaneously; rmaceutically acceptable salt thereof. -2- A compound of Claim 1 in which A is selected from the following: or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, chloro, bromo, fluoro, iodo, 1-imidazolyl,. carboxyl or hydrogen, with the proviso that R4 and R5 cannot be hydrogen simultaneously; or a pharmaceutically acceptable salt thereof. -3- A compound of Claim 1 in which A is selected from the following: or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, chloro, bromo, fluoro, iodo, 1-imidazolyl,. carboxyl or hydrogen, with the proviso that R4 and R5 cannot both be hydrogen simultaneously; or a pharmaceutically acceptable salt thereof. 3-Alkylamino-4-[(substituted phenyl)amino]-cyclobut-3-ene-l,2-dione in which said alkyl group contains 1 to 6 carbon atoms and the phenyl group is substituted by 1 or 2 cyano, nitro, amino, halo or carboxyl group. A compound of Claim 1 which is: 3-(pyridin-4-ylamino)-4-( 1 ,2,2-trimethyl-propylamino)-cyclobut-3-ene- 1 ,2-dione; (exo)-3-(bicyclo[2.2.1]hept-2-ylamino)-4-(pyridin-4-ylamino)-cyclobut-3-ene-l,2- dione; 3-(pyridin-3-ylamino)-4-(l,2,2-trimethyl-propylamino)-cyclobut-3-ene-l,2-dione; 3-(2-methylethylamino)-4-(pyridin-3-ylamino)(cyclobut-3-ene-l,2-dione; 3-dimethylamino-4-(pyridin-3-ylamino)-cyclobut-3-ene- 1 ,2-dione; 3-amino-4-(pyridin-3-ylamino)-cyclobut-3-ene-l,2-dione; or 3-tert-butylamino-4-(pyridin-3-ylamino)-cyclobut-3-ene-l,2-dione. -6- A compound of Claim 1 which is: 3-tert-butylamino-4-(isoquinolin-5-ylamino)-cyclobut-3-ene- 1 ,2-dione; 3-amino-4-(isoquinolin-5-ylamino)-cyclobut-3-ene- 1 ,2-dione; 3-(quinolin-8-ylamino)-4-(l,2,2-trimethyl-propylamino)-cyclobut-3-ene-l,2-dione; 3-methylamino-4-(quinolin-8-ylamino)-cyclobut-3-ene-l,2-dione; 3-amino-4-(quinolin-8-ylamino)-cyclobut-3-ene- 1 ,2-dione; 3-(quinolin-3-ylamino)-4-(l,2,2-trimethyl-propylamino)-cyclobut-3-ene-l,2-dione; 3-tert-butylamino-4-(quinolin-3-ylamino)-cyclobut-3-ene-l,2-dione; 3-(quinolin-3-ylamino)-4-( 1 , 1 -dimethyl -propylamino)-cyclobut-3-ene- 1 ,2-dione; 3-(6-methoxy-quinolin-8-ylamino)-4-(l,2,2-trimethyl-propylamino)-cyclobut-3-ene- 1,2-dione; 3-(6-methoxy-quinolin-8-ylamino)-4-methylamino-cyclobut-3-ene-l,2-dione; 3-tert-butylamino-4-(quinolin-6-ylamino)-cyclobut-3-ene-l,2-dione; or 3-(isoquinolin-5-ylamino)-4-methylamino-cyclobut-3-ene-l,2-dione. -1- A compound of Claim 1 which is: 3-tert-butylamino-4-(lH-indazol-6-ylamino)-cyclobut-3-ene-l,2-dione; (exo)-4-[2-(bicyclo[2.2.1]hept-2-ylamino)-3,4-dioxo-cyclobut-l-enylamino]- benzonitrile; or 4-[3,4-dioxo-2-(l,2,2-trimethyl-propylamino)-cyclobut-l-enylamino]- benzenesulfonamide. -8- A compound of Claim 1 which is 4-[3,4-dioxo-2-(l,2,2-trimethyl- propylamino)-cyclobut- 1 -enylamino] -benzonitrile. -9- A compound of Claim 1 which is (+)-(R)-4-[3,4-Dioxo-2-( 1,2,2- trimethyl-propylamino)-cyclobut-l-enylamino]-3-ethyl-benzonitrile. -10- A compound of Claim 1 which is (+)-(R)-4-[3,4-Dioxo-2-(l,2,2-trimethyl- propylamino)-cyclobut- 1 -enylamino] -3-methoxy-benzonitrile. -11- A method for reducing the adverse effects of smooth muscle contractions which comprises administering, orally or parenterally, to a patient in need thereof, a compound of the formula: wherein: Rl and R2 are, independent from each other, hydrogen, Ci-io straight chain alkyl, Cl-lO branched alkyl , or C3-10 cyclic or bicyclic alkyl; A is is selected from the group consisting of: wherein: R3 is hydrogen, Cl-6 alkyl, Cl-6 perfluoroalkyl, Cl-6 alkoxy, Cl-6 perfluoroalkoxy, amino, Cl- alkylamino, C2-12 dialkylamino, Cl-6 alkylsulfonamido, alkylcarboxamido containing 2 to 7 carbon atoms, nitro, cyano, carboxyl; or, A is a substituted phenyl group of the following formula: wherein: R4 and R5, independent from each other, are selected from the following: cyano, nitro, amino, Cl-6 alkyl, Cl-6 perfluoroalkyl, Cl-6 alkoxy, Cl-6 perfluoroalkoxy, amino, Cl-6 alkylamino, C2- 12 dialkylamino, sulfamyl, Cl-6 alkylsulfonamido, C6-12 arylsulfonamido, alkylcarboxamido containing 2 to 7 carbon atoms, arylcarboxamido containing 7 to 13 carbon atoms, Cl-6 alkylsulfonyl, Cl-6 perfluoroalkylsulfonyl, C6-12 arylsulfonyl, chloro, bromo, fluoro, iodo, 1- imidazolyl, carboxyl or hydrogen, with the proviso that R4 and R5 cannot be hydrogen simultaneously; or a pharmaceutically acceptable salt thereof. -12- The method of Claim 10 in which the smooth muscle adversely contracting causes urinary incontinence. -13- The method of Claim 10 in which the smooth muscle adversely contracting causes irritable bowel syndrome. 14 - A pharmaceutical composition comprising a compound as claimed in any one of Claims 1 to 8 in combination or association with a pharmaceutically acceptable carrier. - 15 A process for preparation of a compound as claimed in Claim 1, which comprises reaction of a compound having the formula (Ila): where Ai is A, as defined in Claim 1, or a group of atoms convertible thereto, and X is a leaving group; with a compound having the formula (IV): R a1 HN R a2 (IV) wherein R a l and R a 2 are, respectively, Rl and R2, as defined in Claim 1, or a group of atoms convertible thereto and, where appropriate, converting Al into A or converting R a ι and Rl or converting R 2 into R2 and, where desired, converting a compound having formula (I) into a pharmaceutically acceptable salt thereof or converting a salt of a compound having formula (I) into a compound having formula (I).

Inventors: ANTANE SCHUYLER ADAM, BUTERA JOHN ANTHONY, ANTANE, SCHUYLER, ADAM, BUTERA, JOHN, ANTHONY
Applicants: AMERICAN HOME PROD, AMERICAN HOME PRODUCTS CORPORATION
Application year: 1994
Main classification: C07D213
IPC codes: A61K31, A61P9, A61P11, C07C255, C07C311, C07D213, C07D215, C07D217, C07D231