ISOXAZOLINE COMPOUNDS AS ANTIINFLAMMATORY AGENTS

Abstract: This invention relates to new isoxazoline compounds which are selective inhibitors of phosphodiesterase type IV (PDEIV). The new isoxazoline compounds are useful in inhibiting PDEIV and in the treatment of AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis, dermatitis, shock, atopic dermatitis, rheumatoid arthritis and osteoarthritis. This invention also relates to pharmaceutical compositions useful therefor.

Description: ISOXAZOLINE COMPOUNDS AS ANTIINFLAMMATORY AGENTS Background of the Invention This invention relates to a series of 3-aryl-2-isoxazoline-5-hydroxamic acid compounds which are selective inhibitors of phosphodiesterase type IV (PDE^) and as such are useful in the treatment of AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis, dermatitis and other inflammatory conditions. This invention also relates to the pharmaceutically acceptable salts of said compounds; to a method of using such compounds in inhibiting PDE, V , and in the treatment of inflammatory conditions, AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis and dermatitis in mammals, especially humans; and to pharmaceutical compositions useful therefor. The "inflammatory conditions" which can be treated according to this invention include, but are not limited to, chronic obstructive pulmonary disease, shock, atopic dermatitis, bronchitis, rheumatoid arthritis and osteoarthritis. Since the recognition that cyclic AMP is an intracellular second messenger (E. W. Sutherland, and T. W. Rail, Pharmacol. Rev., 1960, 12, 265), inhibition of the phosphodiesterases has been a target for modulation and, accordingly, therapeutic intervention in a range of disease processes. More recently, distinct classes of PDE have been recognized (J. A. Beavo and D. H. Reifsnyder, TIPS. 1990, H, 150), and their selective inhibition has led to improved drug therapy (C. D. Nicholson, R. A. Challiss and M. Shahid, TiPS. 1991 , 12, 19). More particularly, it has been recognized that inhibition of PDE^ can lead to inhibition of inflammatory mediator release (M. W. Verghese et al., J. Mol. Cell Cardio 1989, 12 (Suppl. II), S 61) and airway smooth muscle relaxation (T. J. Torphy in Directions for New Anti-Asthma Drugs, eds S. R. O'Donnell and C. G. A. Persson, 1988, 37, Birkhauser-Verlag). Thus, compounds that inhibit PDE W , but which have poor activity against other PDE types, would inhibit the release of inflammatory mediators and relax airway smooth muscle without causing cardiovascular effects or antiplatelet effects. Certain pyrimidone compounds have been disclosed to be useful as antidepres- sants by Saccomano et al., in European Patent Application EPO 247725 A2 published December 2, 1987. The same pyrimidone compounds have been disclosed to be useful against asthma and certain skin disorders in International Patent Application No. PCT/US90/02162, published May 30, 1991 as International Publication Number WO 91/07178. Summary of the Invention This invention is concerned with a series of 3-aryl-2-isoxazoline-5-hydroxamic acid compounds and to the pharmaceutically acceptable salts of such compounds. These new compounds possess inhibitory activity against PDE, V and as such are useful in treating inflammatory conditions, AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis or dermatitis in a mammal, especially humans. The compounds of the present invention are of the formula (I) ( I ) the racemic, racemic-diastereomeric mixtures and optical isomers of said compounds and the pharmaceutically acceptable salts thereof wherein m is 0, 1 , 2 or 3; n is 0, 1 , 2 or 3; Y 1 and Y 2 are independently selected from the group consisting of hydrogen, (C C g )alkyl, optionally substituted phenylalkyl having 1 to 6 carbons in the alkyl portion, optionally substituted phenoxyalkyl having 1 to 6 carbons in the alkyl portion, (C 3 - C 7 )cycloalkyl, difluoromethyl, trifluoromethyl, fluoro, chloro, bromo, iodo, -OR 1 and -OR 2 ; wherein the aromatic portion of the optionally substituted phenylalkyl, and the aromatic portion of the optionally substituted phenoxyalkyl are optionally independently substituted with (C 1 -C 4 )alkyl, (C C 4 )alkoxy, halogen or CF 3 ; R 1 is (C- j -C^alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion fluoromethyl, difluoromethyl, trifluoromethyl, or -(CH 2 ) q -quinoline wherein q is 1 , 2 or 3; R 2 is (C^C g Jalkyl, (C 3 -C 7 ) cycloalkyl, alkoxyalkyl having 3 to 7 carbons in the alkoxy portion and 2 to 4 carbons in the alkyl portion, optionally substituted phenoxyalkyl having 2 to 6 carbons in the alkyl portion, optionally substituted phenylalkyl having 1 to 6 carbons in the alkyl portion, bicycloalkyl having 6 to 9 carbons or optionally substituted indanyl; wherein the aromatic portion of the optionally substituted phenylalkyl, the aromatic portion of the optionally substituted phenoxyalkyl and the optionally substituted indanyl are optionally substituted with (O,-C 4 )alkyl, (C C 4 )alkoxy, halogen or CF 3 ; R 3 is hydrogen, (O,-C 3 )alkyl, fluoro(C 1 -C 3 )alkyl having 1 to 3 fluoro atoms, mono- hydroxyalkyl having 1 to 3 carbons or alkoxyalkyl having 1 to 3 carbons in the alkyl portion and 1 to 3 carbons in the alkoxy portion; R 4 is hydrogen, (C^C^alkyl, fluoro^-C^alkyl having 1 to 3 fluoro atoms, mono- hydroxyalkyi having 1 to 3 carbons, phenyl, alkoxyalkyl having 1 to 3 carbons in the alkyl portion and 1 to 3 carbons in the alkoxy portion, aminoalkyl having 1 to 3 carbons, . r μ wherein X 1 is (C j -C 8 jalkyl and n is an integer from 1 to 3, N-alkylaminoalkyl having 1 to 3 carbons in the alkylamino portion and 1 to 3 carbons in the alkyl portion, (C 3 -C 7 )cycloalkyl or N,N-dialkylaminoalkyl having a total of 2 to 6 carbons in the dialkylamino portion and 1 to 3 carbons in the alkyl portion; R 5 is hydrogen or (C 1 -C 3 )a1kyl; or R 3 and R 4 are taken together with the carbon atoms to which they are attached and form a carbocyclic ring having 4 to 7 carbon atoms. A preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4- position of the phenyl ring; Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring and m, n, R 1 , R 2 , R 3 , R 4 and R 5 are as defined above for formula (I). A more preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring wherein R 1 is (C-,-C 4 )alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) q -quinoline; m is 0; n is 0; and R 2 , R 3 , R 4 and R 5 are as defined above for formula (I). Another more preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring wherein R 1 is (C C 4 )alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) q -quinoline; R 2 is phenylalkyl having 1 to 6 carbons in the alkyl portion, (C 3 -C 7 )cycloalkyl, or (C C 3 )alkyl; m is 0; n is 0; and R 3 , R 4 and R 5 are as defined above for formula (I). An even more preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring wherein R 1 is (C 1 -C 4 )alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) q -quinoline; R 2 is 5-phenylpentyl, benzyl, cyclopentyl or methyl; m is 0; n is 0; and R 3 , R 4 and R 5 are as defined above for formula (I). A particularly preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring wherein R 1 is (C.,-C 4 )alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) q -quinoline; R 2 is 5-phenylpentyl, benzyl, cyclopentyl or methyl; m is 0; n is 0; R 3 is hydrogen and R 4 and R 5 are as defined above for formula (I)- Another particularly preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3- position of the phenyl ring wherein R 1 is (C^C alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) q -quinoline; R 2 is 5-phenylpentyl, benzyl, cyclopentyl or methyl; m is 0; n is 0; R 3 is hydrogen; R 4 is hydrogen or (C^C^alkyl and R 5 is as defined above for formula (I). A more particularly preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3- position of the phenyl ring wherein R 1 is (C j -C^alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) q -quinoline; R 2 is 5-phenylpentyl, benzyl, cyclopentyl or methyl; m is 0; n is 0; R 3 is hydrogen; R 4 is hydrogen or (C-^C g Jalkyl and R 5 is hydrogen or compound or the pharmaceutically acceptable salt thereof of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring wherein R 1 is methyl; R 2 is cyclopentyl; m is 0; n is 0; R 3 is hydrogen; R 4 is hydrogen; and R 5 is hydrogen; and the levorotatory (negative rotation) isomer of a compound or the pharmaceutically acceptable salt thereof of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3- position of the phenyl ring wherein R 1 is methyl; R 2 is cyclopentyl; m is 0; n is 0; R 3 is hydrogen; R 4 is hydrogen; and R 5 is hydrogen. Another more particularly preferred group of compounds or the pharmaceutically acceptable salts thereof are those compounds of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3- position of the phenyl ring wherein R 1 is methyl; R 2 is cyclopentyl; m is 0; n is 0; R 3 is hydrogen; R 4 is methyl; and R 5 is hydrogen; and the levorotatory (negative rotation) isomer of a compound or the pharmaceutically acceptable salt thereof of the formula (I) wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring wherein R 1 is methyl; R 2 is cyclopentyl; m is 0; n is 0; R 3 is hydrogen; R 4 is methyl; and R 5 is hydrogen. The term alkyl encompasses both straight and branched chains. The aromatic portion of the optionally substituted phenylalkyl, the aromatic portion of the optionally substituted phenoxyalkyl and the optionally substituted indanyl may be substituted by one or more substituents. Detailed Description of the Invention The compounds of the present invention having the formula (I) are comprised of the racemic, racemic-diastereomeric mixtures and optical isomers of said compounds and the pharmaceutically acceptable salts thereof. The compounds of the present invention, having the formula I as defined above, are readily and generally prepared by the following reaction process. To an alcoholic solution of sodium methoxide is added an alcoholic solution of hydroxylamine hydrochloride and a compound of the formula wherein Y 1 , Y 2 , R 3 , R 4 , m and n are as defined above for the compound of formula (I), and X is an alkyl group. The reaction mixture is stirred for about 12 to 24 hours, preferably 16 hours, at room temperature. The solvent is evaporated and the residue is worked-up according to methods well known to those skilled in the art. Y 1 The intermediate ester compounds of the formula wherein Y 1 , Y 2 , R 3 , R 4 , m and n are as defined above for the compound of formula (I), and X is an alkyl group, are synthesized according to the following procedure. To a mixture of N-chlorosuccinimide and pyridine in an inert solvent, such as methylene chloride, is added an oxime of the formula wherein Y 1 and Y 2 are as defined above for formula (I). The mixture is allowed to stir for about 2 to 5 , wherein R 3 and R 4 are as defined above for formula I and X is an alkyl group, is added followed by the addition of triethylamine to the mixture and the mixture stirred for about 2 hours more at room temperature. The reaction is worked up according to methods well known to those skilled in the art. The synthetic method outlined above together with the following examples describe methods which were and can be employed to prepare the compounds of this invention. Where possible, as ascertained by one skilled in the art enabled by this disclosure, pharmaceutically-acceptable acid addition salts of certain compounds of this invention include, but are not limited to, those formed with HCI, HBr, HNO 3 , H 2 SO 4 , H 3 PO 4 , CH 3 SO 3 H, p-CH 3 C 6 H 4 SO 3 H, CH 3 CO 2 H, gluconic acid, tartaric acid, maleic acid and succinic acid. In the case of those compounds of the formula (I) which contain a further basic nitrogen, it will, of course, be possible to form diacid addition salts (e.g., the dihydrochloride) as well as the usual monoacid addition salt. Where possible, as ascertained by one skilled in the art enabled by this disclosure, pharmaceutically- acceptable cationic salts of certain compounds of this invention include, but are not limited to, those of sodium, potassium, calcium, magnesium, ammonium, N,N'-dibenzyl- ethylenediamine, N-methylglucamine (meglumine), ethanolamine and diethanolamine. The starting materials and reagents required for the synthesis of the compounds of the present invention are readily available, either commercially, according to literature methods, or by methods exemplified in Preparations below. The ability of the compounds or the pharmaceutically acceptable salts thereof to inhibit PDE^ and, consequently, demonstrate their effectiveness for treating inflammatory conditions is shown by the following in vitro assays. BIOLOGICAL ASSAYS Human Lung PDE^ Thirty to forty grams of human lung tissue is placed in 50 mL of pH 7.4 Tris/phenyl- methylsulfonyl fluoride (PMSF)/sucrose buffer and homogenized using a Tekmar Tissumizer® (Tekmar Co., 7143 Kemper Road, Cincinnati, Ohio 45249) at full speed for 30 seconds. The homogenate is centrifuged at 48,000 x g for 70 minutes at 4°C. The supernatant is filtered twice through a 0.22 μm filter and applied to a Mono-Q FPLC column (Pharmacia LKB Biotechnology, 800 Centennial Avenue, Piscataway, New Jersey 08854) pre-equilibrated with pH 7.4 Tris/PMSF buffer. A flow rate of 1 mL/minute is used to apply the sample to the column, followed by a 2 mL/minute flow rate for subsequent washing and elution. Sample is eluted using an increasing, step-wise NaCI gradient in the pH 7.4 Tris/PMSF buffer. Eight mL fractions are collected. Fractions are assayed for specific PDE^ activity, determined by [ 3 H]cAMP hydrolysis and the ability of a known PDE^ inhibitor (e.g. rolipram) to inhibit that hydrolysis. Appropriate fractions are pooled, diluted with ethylene glycol (2 mL ethylene glycol/5 mL of enzyme prep) and stored at -20°C until use. Compounds are dissolved in DMSO at a concentration of 10 mM and diluted 1 :25 in water (400 μM compound, 4% DMSO). Further serial dilutions are made in 4% DMSO to achieve desired concentrations. Final DMSO concentration in the assay tube is 1 %. In duplicate the following are added, in order, to a 12 x 75 mm glass tube (all concentrations are given as final concentrations in assay tube), i) 25 μl compound or DMSO (1%, for control and blank) ii) 25 μl pH 7.5 Tris buffer iii) [ 3 H]cAMP (1 μM) iv) 25 μl PDE )V enzyme (for blank, enzyme is preincubated in boiling water for 5 minutes) The reaction tubes are shaken and placed in a water bath (37°C) for 20 minutes, at which time the reaction is stopped by placing the tubes in a boiling water bath for 4 minutes. Washing buffer (0.5 mL, 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)/0.1 M NaCI, pH 8.5) is added to each tube on an ice bath. The contents of each tube are applied to an Affi-Gel 601 column (Biorad Laboratories, P.O. Box 1229, 85A Marcus Drive, Melville, New York 11747) (boronate affinity gel, 1 mL bed volume) previously equilibrated with washing buffer. [ 3 H]cAMP is washed with 2 x 6 mL washing buffer, and [ 3 H]5'AMP is then eluted with 4 mL of 0.25M acetic acid. After vortexing, 1 mL of the elution is added to 3 mL of scintillation fluid in a suitable vial, vortexed and counted for [ 3 H]. % Inhibition is determined by the formula: % Inhibition = 1 - average com (test compound) - average com (blank) x 100 average cpm (control) - average cpm (blank) IC^ j is defined as that concentration of compound which inhibits 50% of specific hydrolysis of [ 3 H]cAMP to [ 3 H]5'AMP. For administration to humans to inhibit PDE W and in the treatment of inflammatory conditions, AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis or dermatitis, oral dosages of the compounds of formula (I) or the pharmaceutically acceptable salts thereof, are generally in the range of from 0.1 - 500 mg daily for an average adult patient (70 kg). Thus for a typical adult patient, individual tablets or capsules contain from 0.1 to 50 mg of active compound, in a suitable pharmaceutically acceptable vehicle or carrier. Multiple tablets or capsules may be required to meet the dosage requirements. Dosages for intravenous administration are typically within the range of 0.1 to 10 mg per single dose as required. For intranasal or inhaler administration, the dosage is generally formulated as a 0.1 to 1 % (w/v) solution. In practice the physician will determine the actual dosage which will be most suitable for an individual patient and it will vary with the age, weight and response of the particular patient. The above dosages are exemplary of the average case but there can, of course, be individual instances where higher or lower dosage ranges are merited, and all such dosages are within the scope of this invention. For human use, the compounds of the formula (I) and the pharmaceutically acceptable salts thereof can be administered alone, but will generally be administered in an admixture with a pharmaceutical diluent or carrier selected with regard to the intended route of administration and standard pharmaceutical practice. For example, they may be administered orally in the form of tablets containing such excipients as starch or lactose, or in capsules or ovales either alone or in admixture with excipients, or in the form of elixirs or suspensions containing flavoring or coloring agents. They may be injected parenterally; for example, intravenously, intramuscularly or subcutaneously. For parenteral administration, they are best used in the form of a sterile aqueous solution which may contain other substances; for example, enough salts or glucose to make the solution isotonic. For topical administration, they are best used in the form of solutions, lotions, ointments, salves and the like. Thus in a further aspect the invention provides pharmaceutical compositions comprising a compound of the formula (I), or a pharmaceutically acceptable salts thereof, together with a pharmaceutically acceptable diluent or carrier which are useful: in inhibiting PDE^; in the treatment of inflammatory conditions and in the treatment of AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis and dermatitis in mammals, especially humans. This invention also provides methods of inhibiting PDE^ in a mammal in need thereof which methods comprise administering to said mammal a PDE )V inhibiting amount of a compound of the formula (I) or a pharmaceutically acceptable salt thereof. This invention further provides a method of treating an inflammatory condition in a mammal in need thereof which comprises administering to said mammal an antiinflammatory amount of a compound of the formula (I) or a pharmaceutically acceptable salt thereof. Further still, this invention provides a method of treating AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis, dermatitis or shock in a mammal in need thereof which comprises administering to said mammal an effective amount of a compound or a pharmaceutically acceptable salt thereof. The present invention is illustrated by the following examples, but it is not limited to the details thereof. EXAMPLE 1 3-(3-Cvclopentyloxy-4-methoxy)phenyl-2-isoxazoline-5-hvdroxamic Acid To a solution of sodium methoxide, prepared from 97 mg (4.2 mmol) of sodium and 10 mL of methanol, was added 146 mg (2.1 mmol) of hydroxylamine hydrochloride in a solution of 3 mL of methanol followed by 500 mg (1.5 mmol) of the compound of Preparation 10. After stirring for about 16 h at RT, the solvent was evaporated and the residue was dissolved in 50 mL of water and washed with ether (2 x 50 mL). The aqueous layer was acidified to pH 1 with aqueous HCI solution and the precipitate (231 mg) was filtered and recrystallized twice from C ^CI^EtOAc to give 52 mg of the title compound, mp 167-168°C. 1 H NMR (DMSO-d 6 ): δ 1.54-1.92 (8H, m), 3.48-3.67 (2H, m), 3.78 (3H, s), 4.79-4.85 (1 H, m), 4.95 (1 H, t, J=8), 6.99 (1 H, d, J=9), 7.17 (1 H, d, J=9), 7.23 (1 H, s), 9.03 (1 H, s); Anal. Calc'd. for C^H^N^: C, 59.99; H, 6.29; N, 8.74. Found: C, 59.82; H, 6.05; N, 8.65. EXAMPLES 2 - 16 The following compounds having the formula shown below were prepared substantially according to the procedure of Example 1 substituting the indicated ester for that of the ester of Preparation 10. In the case of Example 5, N-methyl- hydroxylamine hydrochloride was substituted for hydroxylamine hydrochloride. Ex Y 1 Y 2 R 4 R 5 Ester M.P. (°C) Data 2 -OMe -O(CH 2 ) 5 Ph H H Cmpd. of 130-132 Anal. Calc'd for Prep 8 C 22 H 26 N 2° 5 . C, 66.32; H, 6.58; N, 7.03. Found: C, 66.23; H, 6.50; N, 6.94 3 -OMe -O(CH 2 ) 5 Ph Et H Cmpd. of 169-171 Anal. Calc'd for Prep. 9 C 24 H 3θ N 2 O 5 .VH 2 O: C, 66.82; H, 7.07; N, 6.49. Found: C, 67.13; H, 7.03; N, 6.15 Ex Y 1 Y 2 R 4 R 5 Ester M.P. (°C) Data 4 -OMe -°o Me H Cmpd. of 171-173 Anal. Calc'd for Prep. 12 C 17 H 22 2°5 ,1/2H 2° : C, 59.41 ; H, 6.70; N, 8.15. Found: C, 59.78; H, 6.38; N, 8.27 5 -OMe -°-o H Me Cmpd. of 146-148 Anal. Calc'd for Prep. 11 C 17 H 22 N 2°5 : C, 61.07; H, 6.63; N, 8.38. Found: C, 60.87; H, 6.52; N, 8.45 6 -OMe -OMe H H Cmpd. of 180-182 Anal. Calc'd for Prep. 22 C 12 H 14 N 2 0 5 : C, 54.13; H, 5.30; N, 10.52. Found: C, 54.03; H, 5.12; N, 10.60 7 -OMe -OCH 2 Ph H H Cmpd. of 166-168 Anal. Calc'd for Prep. 23 C 18 H 18 N 2 0 5 : C, 63.15; H, 5.30; H, 8.18. Found: C, 63.32; H, 5.37; H, 8.09 8 H H H Cmpd. of 1 H NMR (DMSO- Prep. 24 d g ): δ 3.48-3.68 (2H, m), 4.95 (1 H, t, J=8), 5.43 (2H, s), 7.15 (2H, d, J=9), 7.59-7.69 (4H, m), 7.80 (1H, t, J=8), 8.01 (2H, t, J=7), 8.42 (1H, d, J=9), 9.04 (1 H, S), 10.99 (1H, s). MS (m/e): 363 (M + ) 9 -OCH 2 Ph H H H Cmpd. of 190-192 1 H NMR (DMSO- Prep. 25 dg): d 3.44-3.63 (2H, m), 4.94 (1 H, t, J=8), 5.15 (2H, S), 7.08 (2H, d, J=8), 7.32-7.46 (5H, m), 7.62 (2H, d, J=8), 9.03 (1H, s); MS (m/e): 313 (M + + 1) Ex Y 1 Y 2 R 4 R 5 Ester M.P. (°C) Data 10 H -o H H Cmpd. of 151-153 Anal. Calc'd for Prep. 13 C 15 H 18 N 2 0 4 : C, 62.06; H, 6.25; N, 9.65. Found: C, 62.00; H, 6.15; N, 9.36 11 -o -OMe H H Cmpd. of 136-138 Anal. Calc'd for Prep. 14 C 16 H 20 N 2°5 : c - 59.99; H, 6.29; N, 8.74. Found: C, 59.66; H, 6.21; N, 8.69 12 H H H H Cmpd. of 166-168 Anal. Calc'd for Prep. 17 C 10 H 10 N 2°3 : c - 58.25; H, 4.89; N, 13.59. Found: C, 58.24; H, 4.49; N, 13.45 13 OMe -°-o Pr H Cmpd. of 154-157 Anal. Calc'd for Prep. 18 C 19 H 26 N 2 0 5 : C, 62.97; H, 7.23; N, 7.73. Found: C, 62.61 ; H, 7.19; N, 7.54. 14 OMe -- Bu H Cmpd. of 135-138 HRMS. Calc'd for Prep. 19 C 20 H 28N 2 ° 5 : 376.19982. Found: 376.20104. 15 OMe Ph H Cmpd. of 180-182 Anal. Calc'd for -°-Ό Prep. 20 C→-→H→. N→.O→ C, 66.65; H, 6.10; N, 7.07. Found: C, 66.32; H, 6.30; N, 7.12. 16 Cmpd. of 111-133 HRMS: Calc'd for OMe Prep. 21 C 19 H 24 N 2°5 : 360.1685; Found: 360.1684. CONHOH EXAMPLES 17-22 The following compounds having the formula shown below were prepared substantially according to the procedure of Example substituting the indicated ester for that of the ester of Preparation 10. neO Example Ester R 3 R 4 m MP (°C) Anal. Cmpd. of Prep. # t . ] " 0 17 28 H H 0 137-140° +77° Calc'd. for C^H^N^: C, 59.99; H, MeOH 6.29; N, 8.74. Found: C, 59.98; H, 6.62; N, 8.80. 15 18 29 H H 0 138-140° -82° Calc'd. for C^H^ .^: C, 59.99; H, MeOH 6.29; N, 8.74. Found: C, 59.66; H, 6.44; N, 8.61. Example Ester R 3 R 4 m MP (°C) r l 25 Anal. Cmpd. of Prep. # l a J 0 19 33 H H 1 139-141° racemic Calc'd. for C^H^N^^ΛH jj O; C, 60.02; H, 6.64; N, 8.26. Found: C, 59.63; H, 6.48; N, 8.15. 20 β 31 Me H 0 184-186° racemic Calc'd. for C^H^N^: C, 61.01 ; H, 6.58; N, 8.38. Found: C, 61.08; H, 6.88; N, 8.04. 21 39 H Me 0 167-168° +9° b Calc'd. for C^H^N^-'ΛH jj O: C, CHCI 3 60.25; H, 6.69; N, 8.27. Found: C, 60.43; H, 6.70; N, 8.23. 22 40 H Me 0 153-155° -14° b Calc'd. for C.-Η-→-N-p^H-p C, CHCI 3 59.41 ; H, 6.70; N, 8.15. Found: C, 59.64; H, 6.65; N, 8.03. trans isomer b These compounds are the resolved enantiomers of Example 4. The enantiomeric purity was 99% as determined by chiral HPLC usin a Chrom Tech chiral column. Mobile phase: 98:2 10 mM ammonium acetate buffer, pH 4.1: 2-propanol; flow rate: 1mL/mi detection: 230 nM; temperature: ambient; injection volume: 20 μL. PREPARATION 1 4-Methoxy-3-(5-phenylpentyloxy)benzaldehyde Oxime A mixture of 25.0 g (0.164 mol) of isovanillin, 26.9 g (0.164 mol) of 5-phenyl-1- pentanol, 64.5 g (0.246 mol) of triphenylphosphine and 250 mL of THF was treated dropwise with 42.8 g (0.246 mol) of diethyl azodicarboxylate. The mixture was heated to about 90°C for about 6 hrs and then stirred overnight at RT. The solvent was evaporated and the residue was diluted with 500 mL of EtOAc, washed with water (1 x 400 mL), 1 N NaOH solution (2 x 400 mL), brine (1 x 400 mL), dried (MgSO 4 ), and evaporated to 119 g of a brown oil. Purification by flash chromatography (750 g of silica gel) using an EtOAc-hexane (3:7) eluant afforded 29.8 g (61 %) of an oil. 1 H NMR (CDCI 3 ): δ 1.42-1.92 (6H, m), 2.61 (2H, t, J=7), 3.91 (3H, s), 4.03 (2H, t, J=7), 6.91 (1 H, d, J=8), 7.10-7.40 (m, 7H), 9.77 (s, 1 H). To a solution of 29.8 g (0.100 mol) of the above aldehyde in 300 mL of 95% ethanol was added 13.7 g (0.197 mol) of hydroxylamine hydrochloride in 100 mL of water followed by 16.6 g (0.197 mol) of sodium bicarbonate in small portions (gas evolution!). The mixture was stirred for about 4 h at RT and the ethanol was removed by evaporation. The residue was diluted with 250 mL of water and extracted with EtOAc (2 x 200 mL). The combined extracts were dried (MgSO 4 ) and evaporated to a yellow oil which was crystallized from hexane/ether to afford 15.0 g of the title compound, mp 65-67°C. 1 H NMR (CDCI 3 ): δ 1.46-1.93 (6H, m), 2.62 (2H, t, J=7), 3.88 (3H, s), 4.02 (2H, t, J=7), 6.99-7.62 (m, 6H), 7.49 (1 H, s), 8.04 (1 H, s). An additional 2.00 g of product was obtained as a second crop from the filtrate, mp 67-69°C. Evaporation of the filtrate and purification of the residue by flash chromatography using an EtOAc-hexane (2:3) eluant also provided an additional 4.18 g of product, mp 64-66°C. PREPARATIONS 2 - 4 The following compounds having the formula shown below were prepared, substantially according to the procedure of Preparation 1 , substituting the indicated phenol for isovanillin and the indicated alcohol for 5-phenyl-1 -pentanol. Compounds that were oils were purified by flash chromatography. Prep Y 1 Y 2 Phenol Alcohol M.P.fC) 1 H NMR (CDCI g ) δ: # 2 -OMe -°-o isovanillin cyclopentanol oil 1.50-2.02 (8H, m), 3.94 (3H, S), 4.62-4.80 (1H, m), 6.91 (1H, d, J=8), 6.97 (1H, dd, J=8 and 1), 7.17 (1H, d, J=1), 8.02 (1H, S), 8.16 (1H, s) 3 H →- m- cyclopentanol oil 1.50-1.95 (8H, m), 4.70- hydroxy- 4.78 (1H, m), 6.88 (1H, benzalde- dd, J=3, 8), 7.05-7.28 hyde (3H, m), 8.09 (1 H, s), 8.43 (1H,S) 4 --o -OMe vanillin cyclopentanol 110-111 1.55-2.02 (8H, m), 3.88 (3H, S), 4.78-4.88 (1 H, m), 6.86 (1H, d, J=8), 7.01 (1H, dd, J=2, 8), 7.21 (1 H, d, J=2), 7.65 (1 H, S), 8.07 (1H, S) PREPARATIONS 5 - 6 The following compounds having the formula shown below were prepared by condensation of the indicated aldehyde with hydroxylamine hydrochloride, substantially according to the procedure of Preparation 1. Prep Y 1 Y 2 Aldehyde M.P.(°C) 1 H NMR (DMSO-dg) δ: # 5 -OMe -OH isovanillin 146-148 3.77 (3H, S), 6.92 (2H, s), 7.08 (1H, S), 7.96 (1H, s), 9.16 (1H, s), 10.90 (1H. 8) 6 -OH H p-hydroxy- 115-118 6.77 (2H, d, J=9), 7.40 (2H, d, benzaldehyde J=9), 8.00 (1H, s), 9.74 (1H, s), 10.83 (1H, S) PREPARATION 7 Ethyl 2-Methylenebutyrate A mixture of 5.0 g (0.019 mol) of triethyl 2-phosphonobutyrate, 5.5 g (0.039 mol) of I^CO g , 6.2 g (0.076 mol) of 37% aqueous formaldehyde solution, and 15 mL of water was heated to about 80°C for about 45 min. After cooling to RT, 75 mL of ether was added and the organic layer was separated, washed with brine (1 x 20 mL), dried (MgSO 4 ), and filtered. The ether was carefully removed by distillation, leaving behind 2.1 g (87%) of the title compound as a clear oil which was used directly without further purification. 1 H NMR (CDCI 3 ): δ 1.01 (3H, t, J=7), 1.24 (3H, t, J=7), 2.26 (2H, q, J=7), 4.14 (2H, q, J=7), 5.45 (1 H, s), 6.06 (1 H, s). PREPARATION 8 3-f4-Methoxy-3-(5-phenylpentyloxy)lphenyl-2-isoxazoline-5-carboxylic Acid Ethyl Ester To a mixture of 1.28 g (9.57 mmol) of N-chlorosuccinimide, 200 μl of pyridine, and 200 mL of CH 2 CI 2 was added 2.00 g (6.38 mmol) of the compound of Preparation 1 in a solution of 15 mL of CH 2 CI 2 . An exotherm was observed after about 10 min and following about 2 h of stirring at RT, 644 mg (698 μl, 6.38 mmol) of ethyl acrylate was added followed by 966 mg (1.33 ml, 9.57 mmol) of triethylamine. After the exotherm subsided, the mixture was stirred for about 2 h at RT. The mixture was diluted with 250 mL of CH 2 CI 2 and washed with aqueous 1 N HCI solution, sat'd. aqueous NaHCO 3 solution, dried (Na^SO , and evaporated to an oil. Purification by flash chromatography (100 g of a silica gel) using an EtOAc-hexane (2:3) eluant afforded 1.82 g (69%) of the title compound as an oil. 1 H NMR (CDCI 3 ): δ 1.29 (3H, t, J=7), 1.40-1.91 (6H, m), 2.60 (2H, t, J=7), 3.55-3.58 (2H, m), 3.95 (3H, s), 3.99 (2H, t, J=7), 4.22 (2H, q, J=7), 5.05-5.12 (1 H, m), 6.79 (1 H, d, J=8), 6.95-7.31 (7H, m). PREPARATIONS 9-21 The following compounds having the formula shown below were prepared, substantially according to the procedure of Preparation 8, substituting the indicated oxime for that of the oxime of Preparation 1 and the indicated olefin for ethyl acrylate. Prep Y Y 2 R 4 X Oxime Olefin M.P. 1 H NMR (CDCI3) δ or Elemental Analysis: # PC) 9 -OMe 0(CH 2 ) 5 -Ph Et Et Compound of Compound of oil 0.98 (3H, t, J=7), 1.30 (3H, t. J=7), 1.48-2.03 15 Preparation 1 Preparation 7 (8H, m). 2.62 (2H, t, J=7), 3.18 (1H, d, J=17). 3.76 (1H, d. J=17), 3.86 (3H, s), 4.01 (2H, q. J=7), 6.82 (1H, d. J=8), 7.01 (1H. dd. J=2. 8). 7.15-7.33 (6H, m) 10 -OMe -°-o H Et Compound of Ethyl acrylate oil 1.27 (3H, t, J=7), 1.45-2.00 (8H, m). 3.56 (2H, Preparation 2 d, J=10), 3.82 (3H, s), 4.22 (2H. q, J=7), 4.72^.80 (1H, m), 5.09 (1H, t, J=10). 6.78 (1H. d. J=8), 6.98 (1H. d, J=8), 7.32 (1H. s) The R s ows a contaminant which is most likely a product resulting from chlorination of the aromatic ring. PREPARATION 22 3-(3.4-Dimethoxyphenyl)-2-isoxazoline-5-carboxylic Acid Methyl Ester To a solution of 1.5 g (6.00 mmol) of the compound of Preparation 15 in 25 mL of DMF was added 910 mg of K j CO g (6.60 mmol) and 0.41 mL (940 mg, 6.6 mmol) of methyl iodide. The mixture was heated to about 50°C and the progress of the reaction was monitored by TLC. Additional 0.4 mL portions of methyl iodide were added at about 1 and 2 h, respectively. After about 2 h of additional heating, the reaction was cooled, diluted with 250 mL of water, extracted with EtOAc (3 x 100 mL), dried (MgSO 4 ), and evaporated to an oil. Purification by flash chromatography using an EtOAc-hexane (1 :3) eluant afforded 270 mg of the title compound, mp 106-108°C. 1 H NMR (CDCI 3 ): δ 3.59-3.63 (2H, m), 3.79 (3H, s), 3.89 (3H, s), 5.15 (1 H, t, J=8), 6.83 (1 H, d, J=8), 7.03 (1 H, dd, J=2, 8), 7.37 (1 H, d, J=8); MS (m/e): 266 (M + +1). PREPARATIONS 23 - 25 The following compounds having the formula shown below were prepared, substantially according to the procedure of Preparation 22, substituting the indicated phenol for that of Preparation 15 and the indicated alkylating agent for methyl iodide. e Prep Y Y 2 Phenol Alkylating M.P.(°C) Data # Agent 23 OMe OBn Cmpd of PhCH 2 Br 183-185 1 H NMR (CDCIg): δ 3.54- Prep 15 3.58 (2H,m), 3.79 (3H,s), 3,89 (3H,S), 5.10-5.26 (1H,m), 5.13 (2H,S), 6.86 (1H, d, J=9), 7.06 (1 H, dd, J=2,8), 7.32-7.40 (6H,m); MS (m/e): 342 (M + +1) Prep Y 1 Y 2 Phenol Alkylating M.P.(°C) Data # Agent 24 H Cmpd of 112-113 1 H NMR (CDCIg): δ 3.56- Prep 16 3.59 (2H, m), 3.78 (3H, s), 5.12 (1H, t, J=8), 5.34 (2H, s), 7.02 (2H, d, J=9), 7.54- 7.82 (6H, m), 8.06 (1H, d, J=8), 8.17 (1H, d, J=8); MS (m/e): 363 (M + +1) 25 -OCH 2 Ph H Cmpd of PhCH 2 Br 127-128 Anal. Calc'd for Prep 16 C 18 H 17 N0 4 : C, 69.43; H, 5.50; N, 4.50. Found: C, 69.18; H, 5.31 ; N, 4.59 PREPARATION 26 f3aR-(3ag,6g,7«β1-Hexahvdro-8,8-dimethyl-1-(1-oxo-2-propenyl)-3H-3a.6-methano- 2.1-benzisothiazole 2.2-Dioxide The title compound was prepared according to the method of Curran and Heffner (Curran, D. P., Heffner, T.A., J. Org. Chem.. 1990. 55, 4585) starting with (+)-L-2,10- camphor sultam, which was purchased from Fluka. Into a 1 L 3-neck round bottom flask fitted with reflux condenser, N 2 inlet, rubber septum and glass stopper was placed 4.03 g (0.084 mol) of 50% NaH dispersion, 400 mL of toluene, and 12.0 g (0.056 mol) of (+)-10,2-camphor sultam. After stirring for 1 h. at RT, 594 mg (0.006 mol) of CuCI followed by 9.10 mL (0.056 mol) of acryloyi chloride were added and stirring was continued overnight at RT. The mixture was then treated with 15 mL of water, evaporated, diluted with water (200 mL), and extracted with EtOAc (3 x 200 mL). The combined extracts were dried (MgS0 4 ) and evaporated to a solid. Purification by flash chromatography (1 kg of silica gel) using a 3:7 EtOAc- hexane eluant afforded a white solid which was triturated with ether to provide 7.4 g of the title compound, mp 179-182°C. PREPARATION 27 f3aS-(3ag.6g.7g Bl-Hexahvdro-8.8-dimethyl-1 -(1 -oxo-2-propenyl)-3H-3a.6-methano- 2,1-benzisothiazole 2,2-Dioxide The title compound was prepared according to the procedure of Preparation 26, however, starting with (-)-D-2,10-camphor sultam, which was purchased from Fluka. PREPARATIONS 28-31 The following compounds having the formula shown below were prepared substantially according to the procedure of Preparati 8, however, substituting the compound of Preparation 2 for the compound of Preparation 1 and the indicated olefins for ethyl acrylat MeO Prep. R 3 R 4 Q Olefin MP (°C) r 1- α ι as Data J 0 CHCI 3 28 a H H Compound of foam +187° 1 H-NMR(CDCI 3 );δ 0.97 (3H, s), 1.19 (3H, Prep. 26 s), 1.28-2.22 (15H, m), 3.41-3.95 (5H, m), 3.85 (3H, s), 4.74-4.82 (1 H, m), 5.63 (1H, dd, J=8, 10), 6.80 (1H, d, J=8), 7.02 (1 H, dd, J=2, 8), 7.33 (1 H, d, J=2). less polar diastereomer (R, 0.61 ; 1 :1 ether-toluene) .; b trans stereochemistry PREPARATION 32 3-(3-Cvclopentyloxy-4-methoxy) phenyl-2-isoxazoline-5-acetic Acid To a solution of 1.85 g (6.06 mmol) of the compound of Preparation 30 in 50 mL of acetone chilled to about 0°C in an ice bath was added dropwise 9.70 mL (12.1 mmol) of a 1.25 M solution of Jones reagent. The ice bath was allowed to melt, and after about 4 h. of stirring an additional 2.00 mL of Jones reagent was added and stirring was continued overnight. Excess reagent was quenched by the addition of 10 mL of isopropanol, and the solids were removed by filtration. The filtrate was concentrated and the residue was taken up in 150 mL of EtOAc, washed with water (2 x 100 mL), dried (MgSO 4 ), and evaporated to a yellow oil. Crystallization from ether-hexane gave 1.06 g of the title compound, mp 123-126°C. 1 H-NMR (CDCI 3 ): δ 1.55-2.06 (8H, m), 2.66-3.59 (4H, m), 3.87 (3H, s), 4.78-4.87 (1 H, m), 5.02-5.15 (1 H, m), 6.84 (1 H, d, J=8), 7.02 (1 H, dd, J=2, 8), 7.37 (1 H, d, J=2). PREPARATION 33 3-(3-Cvclopentyloxy-4-methoxy)phenyl-2-isoxazoline-5-acetic Acid Methyl Ester A solution of 530 mg of the compound of Preparation 32 in 5 mL of MeOH was saturated with HCI gas and the mixture was stirred for about 3 h. at RT protected from atmospheric moisture with a CaCI 2 tube. The mixture was concentrated and the residue was taken up in 50 mL of EtOAc, washed with saturated aqueous NaHCO 3 solution (2 x 50 mL), dried (MgSO 4 ), and evaporated to 530 mg of an oil. Purification by flash chromatography (25 g of silica gel) using a 2:3 - EtOAc:hexane eluant gave an oil which was crystallized from hexane-ether to afford 323 mg of the title compound as a white solid, mp 78-80°C. Anal. Calc'd. for C 18 H 23 NO 5 ; C, 64.85; H, 6.95; N, 4.20. Found: C, 64.49; H, 7.08; N, 4.13. PREPARATIONS 34-36 The following compounds having the formula shown below were prepared as oils substantially according to the procedure of Preparation 7 substituting the indicated ester for triethylphosphonobutyrate. .Λ C 0 2 E t Prep. # R 4 Ester 1 H-NMR(CDCI 3 ):δ 34 Pr triethyl 0.90 (3H, t, J=7), phosphonopentanoate 1.28 (3H, t, J=7), 1.40-1.53 (2H, m), 2.25 (2H, dt, J=1 and 7), 4.17 (2H, q, J=7), 5.48 (1 H, q, J=1), 6.11 (1 H, t, J=11) 35 Bu triethyl 0.90 (3H, t, J=7), phosphonohexanoate 1.29 (3H, t, J=7), 1.26-1.48 (4H, m), 2.28 (2H, t, J=7), 4.19 (2H, q, J=7), 5.49 (1 H, q, J=1), 6.11 (1 H, t, J=1) 36 Ph triethyl 1.32 (3H, t, J=7), phosphonophenylacetate 4.28 (2H, q, J=7), 5.88 (1 H, d, J=1), 6.34 (1 H, d, J=1), 7.20-7.45 (5H, m) PREPARATIONS 37 and 38 Less Polar Diastereomer of N-[(S)-α-Methylbenzyl]-3-(3-cyclo- pentyloxy-4-methoxy)phenyl-5-methyl-2-isoxazoline-5-carboxamide (Preparation 37) More Polar Diastereomer of N-[(S)-α-Methylbenzyl]-3-(3-Cyclo- pentyloxy-4-methoxy)phenyl-5-methyl-2-isoxazoline-5-carboxamide (Preparation 38) A solution of 5.00 g (14 mmol) of the compound of Preparation 12 in 100 mL of absolute ethanol was treated with 2.36 g (42 mmol) of KOH and the mixture was stirred for about 4 hr at RT. An additional equivalent of KOH was added and stirring was continued for about 3 days. The mixture was concentrated, diluted with water, acidified with aqueous 1 N HCI solution, and extracted with EtOAc (2 x 100 mL). The combined extracts were dried (MgSO 4 ), evaporated, and triturated with hexane-ether to give 3.46 g of 3-[3-cyclopentyloxy-4-methoxy]phenyl-5-methyl-2-isoxazoline-5-carboxylic acid, mp 153-154°. A mixture of 3.00 g (94 mmol) of the above compound, 100 mL of benzene, and 2.46 mL (28.2 mmol) of oxalyl chloride was heated to reflux for about 3 hr. The mixture was concentrated, diluted with 100 mL of CH 2 CI 2 , and treated with 2.42 mL (18.8 mmol) of S-(-)-α-methylbenzylamine. After stirring for about 16 hr at RT, the mixture was concentrated, diluted with 200 mL of EtOAc, washed with aqueous 1 N HCI solution (2 x 100 mL), saturated aqueous NaHCO 3 solution (2 x 100 mL), dried (Na^O , and evaporated. The residual solid (5.76 g) was purified by flash chromatography over 600 g of silica gel using 15-20% ether-toluene as eluant. Following a 500 mL pre-fraction, 35 ml-fractions were collected. Fractions 59-68 were pooled and evaporated to give 630 mg of the compound of Preparation 37, mp 154-156°C; R, 0.20, 20% ether-toluene. Anal, calculated for C^H^N^: C, 71.06; H, 7.16; N, 6.63. Found: C, 71.13; H, 7.42; N, 6.76. Fractions 82-104 were pooled and concentrated to 720 mg of a white solid which was triturated with hexane-ether to give 596 mg of a white solid, mp 165-167°C. Recrystallization from ether-CH 2 CI 2 afforded 435 mg of the compound of Preparation 38, mp 167-168°C. An additional 1.03 g of the compound of Preparation 38, mp 166- 167°C, was obtained by recrystallization (ether-CH 2 CI 2 ) of the combined evaporated residues of the mother liquor and Fractions 69-81. Anal, calculated for C gg H g oN^: C, 71.06; H, 7.16; N, 6.63. Found: C, 70.89; H, 7.40; N, 6.77. PREPARATION 39 (+)-3-(3-Cyclopentyloxy-4-methoxy)phenyl-5- methyl-2-isoxazoline-5-carboxylic Acid Methyl Ester Into a flame-dried, 3-neck round-bottom flask under N 2 was placed a suspension of 549 mg (3.56 mmol) of 26% KH in mineral oil. After removal of the mineral oil by 2 successive hexane washes, the bare hydride was suspended in 35 mL of THF and a solution of 750 mg (1.78 mmol) of the compound of Preparation 37 in 35 mL of dry THF was added dropwise. After the bubbling subsided, 161 μl (2.67 mmol) of carbon disulfide was added. The mixture was stirred for about 16 hr at RT and was quenched by the addition of 6 mL of water. The THF was evaporated and the residue was diluted with saturated aqueous NaHC0 3 solution and washed with EtOAc (2 x 100 mL). The aqueous layer was acidified to pH 3 with aqueous 6N HCI solution, extracted with EtOAc (2 x 100 mL), dried (MgSO^, and evaporated to 217 mg of an orange oil. A solution of the above oil in 20 mL of MeOH was saturated with HCI gas and stirred for about 16 hr at RT. The mixture was concentrated, diluted with 50 mL of EtOAc, dried (MgSO 4 ), and evaporated to a yellow solid. Purification by flash chromatography over 12 g of silica gel using a 60% EtOAc-hexane eluant afforded 131 mg of the title compound after trituration in hexane-ether, mp 127-128°C. [α] D 25 +100° (c = 0.64, CHCy. Anal, calculated for C 18 H 23 NO 5 » 1 /4H 2 O: C, 63.99; H, 7.01 ; N, 4.15. Found: C, 64.03; H, 6.96; N, 4.15. PREPARATION 40 (-)-3-(3-Cvclopentyloxy-4-methoxy)phenyl-5-methyl-2-isoxazoline-5-carboxamide The title compound was prepared substantially according to Procedure 39 substituting the compound of Preparation 38 for the compound of Preparation 37; mp 124-125°C; [α] D 25 -101° (c = 0.61 , CHCI 3 ). Anal calculated for C 18 H 23 NO 5 * /4H 2 O: C, 63.99; H, 7.01 ; N, 4.15. Found: C, 64.04; H, 7.00; N, 4.17.

Claims: CLAIMS What is claimed is: 1. A compound of the formula the racemic, racemic-diastereomeric mixtures and optical isomers of said compounds and the pharmaceutically acceptable salts thereof wherein m is 0, 1 , 2 or 3; n is 0, 1 , 2 or 3; Y 1 and Y 2 are independently selected from the group consisting of hydrogen, (C^ C 6 )alkyl, optionally substituted phenylalkyl having 1 to 6 carbons in the alkyl portion, optionally substituted phenoxyalkyl having 1 to 6 carbons in the alkyl portion, (C 3 - C 7 )cycloalkyl, difluoromethyl, trifluoromethyl, fluoro, chloro, bromo, iodo, -OR 1 and -OR 2 ; wherein the aromatic portion of the optionally substituted phenylalkyl, and the aromatic portion of the optionally substituted phenoxyalkyl are optionally independently substituted with (C^C^alkyl, (C^C^alkoxy, halogen or CF 3 ; R 1 is (O^C alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion fluoromethyl, difluoromethyl, trifluoromethyl, or -(CH 2 ) q -quinoline wherein q is 1 , 2 or 3; R 2 is (C-,-C 3 )alkyl, (C 3 -C 7 )cycloalkyl, alkoxyalkyl having 3 to 7 carbons in the alkoxy portion and 2 to 4 carbons in the alkyl portion, optionally substituted phenoxyalkyl having 2 to 6 carbons in the alkyl portion, optionally substituted phenylalkyl having 1 to 6 carbons in the alkyl portion, bicycloalkyl having 6 to 9 carbons or optionally substituted indanyl; wherein the aromatic portion of the optionally substituted phenylalkyl, the aromatic portion of the optionally substituted phenoxyalkyl and the optionally substituted indanyl are optionally substituted with (C 1 -C 4 )alkyl, (C C 4 )alkoxy, halogen or CF 3 ; R 3 is hydrogen, (O,-C 8 )alkyl, fluoro(O | -C 3 )alkyl having 1 to 3 fluoro atoms, mono- hydroxyalkyl having 1 to 3 carbons or alkoxyalkyl having 1 to 3 carbons in the alkyl portion and 1 to 3 carbons in the alkoxy portion; R 4 is hydrogen, (C^C^alkyl, fluoro(O,-C 5 )alkyl having 1 to 3 fluoro atoms, mono- hydroxyalkyl having 1 to 3 carbons, phenyl, alkoxyalkyl having 1 to 3 carbons in the alkyl portion and 1 to 3 carbons in the alkoxy portion, aminoalkyi having 1 to 3 carbons, and n is an integer from 1 to 3, N-alkylaminoalkyl having 1 to 3 carbons in the alkylamino portion and 1 to 3 carbons in the alkyl portion, (C 3 -C 7 )cycloalkyl or N,N-dialkylaminoalkyl having a total of 2 to 6 carbons in the dialkylamino portion and 1 to 3 carbons in the alkyl portion; R 5 is hydrogen or (C^C g Jalkyl; or R 3 and R 4 are taken together with the carbon atoms to which they are attached and form a carbocyclic ring having 4 to 7 carbon atoms. 2. A compound according to claim 1 or a pharmaceutically acceptable salt thereof wherein Y 1 is -OR 1 and is attached to the 4-position of the phenyl ring and Y 2 is -OR 2 and is attached to the 3-position of the phenyl ring. 3. A compound according to claim 2 or a pharmaceutically acceptable salt thereof wherein R 1 is (C,-C 4 )alkyl, phenylalkyl having one to four carbon atoms in the alkyl portion or -(CH 2 ) -quinoiine; m is 0 and n is 0. 4. A compound according to claim 3 or a pharmaceutically acceptable salt thereof wherein R 2 is phenylalkyl having 1 to 6 carbons in the alkyl portion, (C 3 - C 7 )cycloalkyl, or (C C 3 )alkyl. 5. A compound according to claim 4 or a pharmaceutically acceptable salt thereof wherein R 2 is 5-phenylpentyl, benzyl, cyclopentyl or methyl. 6. A compound according to claim 5 or a pharmaceutically acceptable salt thereof wherein R 3 is hydrogen. 7. A compound according to claim 6 or a pharmaceutically acceptable salt thereof wherein R 4 is hydrogen or (C^C^alkyl. 8. A compound according to claim 7 or a pharmaceutically acceptable salt thereof wherein R 5 is hydrogen or (C^C g Jalkyl. 9. A compound according to claim 8 or a pharmaceutically acceptable salt thereof wherein R 1 is methyl; R 2 is cyclopentyl; R 4 is hydrogen; and R 5 is hydrogen. 10. The levorotatory (negative rotation) enantiomer of the compound according to claim 9 or a pharmaceutically acceptable salt thereof. 11. A compound according to claim 8 or a pharmaceutically acceptable salt thereof wherein R 1 is methyl; R 2 is cyclopentyl; R 4 is methyl; and R 5 is hydrogen. 12. The levorotatory (negative rotation) enantiomer of the compound according to claim 11 or a pharmaceutically acceptable salt thereof. 13. A pharmaceutical composition comprising an effective amount of a compound according to claim 1 or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable diluent or carrier. 14. A method of inhibiting phosphodiesterase type IV in a mammal in need thereof which comprises administering to said mammal a phosphodiesterase type IV inhibiting amount of a compound according to claim 1 or a pharmaceutically acceptable salt thereof. 15. A method of treating an inflammatory condition in a mammal which comprises administering to said mammal an antiinflammatory amount of a compound according to claim 1 or a pharmaceutically acceptable salt thereof. 16. A method of treating AIDS, asthma, arthritis, bronchitis, chronic obstructive pulmonary disease, psoriasis, allergic rhinitis, dermatitis or shock in a mammal which comprises administering to said mammal an effective amount of a compound according to claim 1 or a pharmaceutically acceptable salt thereof.

Inventors: KLEINMAN EDWARD F, KLEINMAN, EDWARD, F.
Applicants: KLEINMAN EDWARD F, PFIZER, KLEINMAN, EDWARD, F., PFIZER INC.
Application year: 1994
Main classification: C07D261
IPC codes: A61K31, A61P11, A61P17, A61P29, A61P37, A61P43, C07D261, C07D413, C07D417