BICYCLO (2.2.2) OCTANE DERIVATIVES CHOLECYSTOKININ AND/OR GASTRIN ANTAGONISTS

Abstract: Compounds of formula (I) wherein R<1> and R<2> are independently H, methyl, halo, carboxy, esterified carboxy, amidated carboxy, carboxymethyl, esterified carboxymethyl or amidated carboxymethyl; R<3> and R<4> (or each R<3> and R<4> group, when m or n is 2 or more) are independently selected from halo, amino, nitro, cyano, sulphamoyl, sulphonyl, trifluoromethyl, C1 to C3 alkyl, C1 to C3 alkoxy, hydroxy, C1 to C3 hydroxyalkyl, C1 to C3 alkylcarboxyamino, carboxy, esterified carboxy and amidated carboxy; R<5> and R<6> are independently selected from H and the groups recited above for R<3>; m is from 0 to 4, provided that m is not more than 2 unless R<3> is exclusively halo; n is from 0 to 4, provided that n is not more than 2 unless R<4> is exclusively halo; R<7>, R<9> and R<10> are independently H or C1 to C3 alkyl; R<8> is H or C1 to C15 hydrocarbyl, in which one or more hydrogen atoms of the hydrocarbyl group may be replaced by a halogen atom, and up to two of the carbon atoms may be replaced by a nitrogen, oxygen or sulphur atom, provided that R<8> does not contain a -O-O-group; U is aryl, substituted aryl, heterocyclic, substituted heterocyclic or cycloalkyl, and Z is a group of formula (II) or (III) (wherein R is H or C1 to C3 alkyl; X is -CO2H or tetrazolyl; Y is H, -CO2H, tetrazolyl, -CH2OH, -CO2Me or -CONH2, and a is from 0 to 2); and pharmaceutically acceptable salts thereof have a high affinity for CCK and/or gastrin receptors.

Description: BICYCLO (2.2.2) OCTANE DERIVATIVES CHOLECYSTOKININ AND/OR GASTRIN ANTAGONISTS This invention relates to bicyclo[2.2.2]octane derivatives, and more particularly to bicyclo[2.2.2]octane derivatives which bind to cholecystokinin and/or gastrin receptors. The invention also relates to methods for preparing such bicyclo[2.2.2]octane derivatives. Gastrin and the CCK's are structurally-related neuropeptides which exist in gastrointestinal tissue and in the CNS (see Mutt V., Gastrointestinal Hormones, Glass G.B.J., ed., Raven Press, N.Y., p 169 and Nisson G., ibid, p. 127) . Gastrin is one of the three primary stimulants of gastric acid secretion. Several forms of gastrin are found including 34-, 17-, and 14-amino acid species with the minimum active fragment being the C-terminal tetrapeptide (TrpMetAspPhe-NH : ) which is reported in the literature to have full pharmacological activity (see Tracey H.J. and Gregory R.A., Nature (London), 1964, 04, 935). Much effort has been devoted to the synthesis of analogues of this tetrapeptide (and the N-protected derivative Boc-TrpMetAspPhe-NH 2 ) in an attempt to elucidate the relationship between structure and activity. Natural cholecystokinin is a 33 amino acid peptide (CCK-33), the C-terminal 5 amino acids of which are identical to those of gastrin. Also found naturally is the C-terminal octapeptide (CCK- 8) of CCK-33. 0 The cholecystokir-ins are reported to be important in the regulation of appetite. They stimulate intestinal motility, gall bladder contraction, pancreatic enzyme secretion, and are known to have a trophic action on the pancreas. They also inhibit gastric emptying and have various effects in the CNS. 5 Compounds which bind to cholecystokinin and/or gastrin receptors are iirportant because of their potential pharmaceutical use as antagonists of the natural peptides. A number of gastrin antagonists have been proposed for various therapeutic applications, including the prevention of gastrin- related disorders, gastrointestinal ulcers, Zollinger-Ellison syndrome, antral G Cell hyperplasia and other conditions in which lowered gastrin activity is desirable. The hormone has also been shown to have a trophic action on cells and so an antagonist may be expected to be useful in the treatment of cancers, particularly in the stomach and the colon. Possible therapeutic uses for cholecystokinin antagonists include the control of appetite disorders such as anorexia nervosa, and the treatment of pancreatic inflammation, biliary tract disease and various psychiatric disorders. Other possible uses are in the potentiation of opiate (e.g. morphine) analgesia, and in the treatment of cancers, especially of the pancreas. Moreover, ligands for cholecystokinin receptors in the brain (so-called CCKg receptors) have been claimed to possess anxiolytic activity. Our earlier co-pending application PCT/GB93/00346 discloses a class of bicyclooctane derivatives which are ligands at gastrin and/or CCK receptors. Included within this class are compounds of the formula (and ring-substituted derivatives thereof) wherein each R group is independently H or C : to C-, alkyl R' is C j to C 15 hydrocarbyl (or a derivative thereof) , R' ' is H or methyl, Ar is an aromatic group, and Z is a substituted amino group. It has now been found that compounds of especially high activity are obtained when Z is a group of the formula wherein R is H or C, to C 3 alkyl, X is -C0 2 H or tetrazolyl, Y is H, -C0 2 H, tetrazolyl? -CH 2 OH, -C0 2 Me or -C0NH 2 , and a is from 0 to 2. According to the present invention, therefore, there are provided compounds of the formula wherein R 1 and R are independently H, methyl, halo, carboxy, esterified carboxy, amidated carboxy, carboxymethyl, esterified carboxymethyl or amidated carboxymethyl, R 3 and R 4 (or each R 3 and R group, when m or n is 2 or more) are independently selected from halo, amino, nitro, cyano, sulphamoyl, sulphonyl, trifluoromethyl, to C 3 alkyl, Cj to C 3 alkoxy, hydroxy1, C- Λ to C 3 hydroxyalkyl, C, to C 3 alkylcarboxyamino, carboxy, esterified carboxy and amidated carboxy R 5 and R 6 are independently selected from H and the groups recited above for R 3 m is from 0 to 4, provided that m is not more than 2 unless R 3 is exclusively halo, n is from 0 to 4, provided that n is not more than 2 unless R 4 is exclusively halo, R 7 , R 9 and R lϋ are independently H or C_ to C 3 alkyl, R 8 is H or Cj to C 15 hydrocarbyl, in which one or more hydrogen atoms of the hydrocarbyl group may be replaced by a halogen atom, and up to two of the carbon atoms may be replaced by a nitrogen, oxygen or sulphur atom, provided that R 8 does not contain a -0-0- group, U is aryl, substituted aryl, heterocyclic (including both saturated and unsaturated groups) , substituted heterocyclic or cycloalkyl, and Z is a group having the formula (II) defined above, and pharmaceutically acceptable salts thereof. The invention also comprehends derivative coπpounds ("pro-drugs") which are degraded in vivo to yield the species of formula (III) . Pro-drugs are usually (but not always) of lower potency at the target receptor than the species to which they are degraded. Pro- drugs are particularly useful when the desired species has chemical or physical properties which make its administration difficult or inefficient. For example, the desired species may be only poorly soluble, it may be poorly transported across the mucosal epithelium, or it may have an undesirably short plasma half-life. Further discussion of pro-drugs may be found in Stella, V. J. et al , "Prodrugs", Drug Delivery Systems, pp. 112-176 (1985), and Drugs, .29, pp.455-473 (1985). Pro-drug forms of the pharmacologically-active compounds of the invention will generally be corrpounds according to formula (III) in which X and/or Y represents an esterified or amidated acid group. Included in such esterified acid groups are groups of the form -COOR 11 , wherein R :ι is C : to C alkyl, phenyl, substituted pheπyl, benzyl, substituted benzyl, heteroaryl or one of the following: Amidated acid groups include groups of the formula -CONR 12 R 13 , wherein R 12 is H, C 1 to C 5 alkyl, phenyl, substituted phenyl, benzyl, or substituted benzyl, and R 13 is -OH or one of the groups just recited for R 12 . The term "hydrocarbyl", as used herein, refers to monovalent groups consisting of carbon and hydrogen. Hydrocarbyl groups thus include alkyl, alkenyl, and alkynyl groups (in both straight and branched chain forms) , cycloalkyl (including polycycloalkyl) , cycloalkenyl, and aryl groups, and combinations of the foregoing, such as alkylaryl, alkenylaryl, alkynylaryl, cycloalkylaryl, and cycloalkenylaryl groups, A "carbocyclic" group, as the term is used herein, comprises one or more closed chains or rings, which consist entirely of carbon atoms. Included in such groups are alicyclic groups (such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and adamantyl), groups containing both alkyl and cycloalkyl moieties (such as adamantanemethyl), and aromatic groups (such as phenyl, naphthyl, indanyl, fluorenyl, (1,2,3,4)-tetrahydronaphthyl, indenyl and isoindenyl) . The term "aryl" is used herein to refer to aromatic carbocyclic groups, including those mentioned above. A "heterocyclic" group comprises one or more closed chains or rings which have at least one atom other than carbon in the closed chain or ring. Examples include benzimidazolyl, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, pyrrolidinyl, pyrrolinyl, imidazolidinyl, imidazolinyl, pyrazolidinyl, tetrahydrofuranyl, pyranyl, pyronyl, pyridyl, pyrazinyl, pyridazinyl, piperidyl, piperazinyl, morpholinyl, thionaphthyl, benzofuranyl, isobenzofuryl, indolyl, oxyindolyl, isoindolyl, indazolyl, indolinyl, 7-azaindolyl, isoindazolyl, benzopyranyl, coumarinyl, isocoumarinyl, quinolyl, isoquinolyl, naphthridinyl, cinnolinyl, quinazolinyl, pyridopyridyl, benzoxazinyl, quinoxadinyl, chromenyl, chromanyl, isochrorranyl and carbolinyl. The term "halogen", as used herein, refers to any of fluorine, chlorine, bromine and iodine. Most usually, however, halogen substituents in the compounds of the invention are chlorine or fluorine substituents. Preferably, m and n are both 0. However, when m and n are not both 0, R 3 and R 4 are preferably selected from halo, hydroxy, amino, nitro, cyano, sulphamoyl, C : to C→. alkyl and to C 3 alkoxy. As mentioned above, when m or n is 2 or more, each R' and R 4 group is independent of the others. For example, the compounds of the invention may include two different R 3 groups. Particularly preferred groups for R-' and R'- are hydrogen and the groups just recited for R ; , and especially hydrogen, methyl and fluoro. When reference is made herein to a "substituted" aromatic group, the substituents will generally be from 1 to 3 in number (and more usually 1 or 2 in number) , and generally selected from the groups recited above for R'. However, halo substituents may be up to 5 in number. Preferably, R 8 is C_ to C. straight or branched chain alkyl or cycloalkyl, or R ι: -(CH_) r -, wherein R ι is selected from phenyl, 1- naphthyl, 2-naphthyl, indolyl, norbo nyl, 1-adamantyl, 2-adamantyl, cyclohexyl or cycloheptyl, and p is from 0 to 3. Pharmaceutically acceptable salts of the acidic compounds of the invention include salts with alkali metals and alkaline earth metals, such as sodium, potassium, calcium and magnesium, and salts with organic bases. Suitable organic bases include amines such as N-methyl-D-glucamine. Pharmaceutically acceptable salts of the basic compounds of the invention include salts derived from organic or inorganic acids. Suitable acids include hydrochloric acid, phosphoric acid, oxalic acid, aleic acid, succinic acid and citric acid. The compounds of the invention exist in various enantiomeric and diastereomeric forms. It will be understood that the invention comprehends the different enantiomers and diastereomers in isolation from each other, as well as mixtures of enantiomers and diastereomers. Also, the structural formulae herein show the groups R 1 and R arranged cis to each other, but it will be appreciated that the invention includes the corresponding trans isomers. Compounds according to the present invention may conveniently be ade by the process depicted in Reaction Scheme A. Reaction Scheme A In this scheme, anthracene or an anthracene derivative (1) is reacted with the acid anhydride (2) in a Diels-Alder reaction. The reactants are conveniently refluxed together in a suitable solvent such as toluene to form the adduct (3) . In some cases, it may be appropriate to conduct the reaction at elevated pressure and/or in the presence of a Lewis acid catalyst. The adduct (3) is then reacted with an amine of formula HNR 7 R 8 to form the acid compound (4) . The reaction is suitably carried out in a solvent such as THF in the presence of a base such as DMAP. The acid compound (4) is then reacted with a compound of formula in which Z' represents a suitably protected form of the group Z, to form protected intermediate (5) . Deprotection of Z' (eg. by hydrogenation over a palladium catalyst) then yields the desired compound (6) . Alternatively, as shown in Reaction Scheme A, the two amidation reactions may be carried out in the reverse order, proceeding via the intermediate (4') . Suitable amidation methods are described in detail in "The Peptides, Vol. 1", Gross and Meinenhofer, Eds., Academic Press, N.Y., 1979. These include the carbodiimide method (using, for example, 1,3-dicyclohexylcarbodiimide [DCC] or l-(3- dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride [EDCI] , and optionally an additive such as 1-hydroxybenzotriazole [HOBT] to prevent racemization) , the azide method, the mixed anhydride method, the symmetrical anhydride method, the acid chloride method, the use of bis (2-oxo-3-oxazolidinyl) phosphinic chloride [BOP-C1] , the use of PyBOP or EyBrOP, the use of the isopropenylsuccinimido carbonate method and the active ester method (using, for example, N-hydroxysuccinimide esters, 4-nitrophenyl esters or 2,4,5-trichlorophenol esters) . The coupling reactions are generally conducted under an inert atmosphere, such as an atmosphere of nitrogen or argon. -Suitable solvents for the reactants include methylene chloride, tetrahydrofuran [THF] , di ethoxyethane [DME] and d-ύ-netbylfonnamide [DMF] . The equivalent trans adducts can be prepared using a suitably differentiated fumaric acid (eg the mono methyl mono benzyl diester) , which, after addition to anthracene or an anthracene derivative (1) , allows independent elaboration of the two sidechains. The invention therefore also provides a method of making a compound according to .formula (III) above, said method including the step of reacting a compound of the formula with a suitably protected compound of formula As already mentioned, it may be desired to carry out the amidation reactions in a different order. The invention therefore also comprehends a method of making a compound according to formula (III) above, said method including the step of reacting a compound of the formula ( with a suitably protected compound of formula HNRR 8 , or the step of reacting a compound of the formula with a suitably protected coπpound of formula Pharmaceutically acceptable salts of the acidic or basic coπpounds of the invention can of course be made by conventional procedures, such as by reacting the free base or acid with at least a stoichiometric amount of the desired salt-forming acid or base. The coπpounds of the invention can be administered by oral or parenteral routes, including intravenous, intramuscular, intraperitoneal, subcutaneous, rectal and topical administration. For oral administration, the compounds of the invention will generally be provided in the form of tablets or capsules or as an aqueous solution or suspension. Tablets for oral use may include the active ingredient mixed with pharmaceutically acceptable excipients such as inert diluents, disintegrating agents, binding agents, lubricating agents, sweetening agents, flavouring agents, colouring agents and preservatives. Suitable inert diluents include sodium and calcium carbonate, sodium and calcium phosphate, and lactose, while corn starch and alginic acid are suitable disintegrating agents. Binding agents may include starch and gelatin, while the lubricating agent, if present, will generally be magnesium stearate, stearic acid or talc. If desired, the tablets may be coated with a material such as glyceryl monostearate or glyceryl distearate, to delay absorption in the gastrointestinal tract. Capsules for oral use include hard gelatin capsules in which the active ingredient is mixed with a solid diluent, and soft gelatin capsules wherein the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin or olive oil. For intramuscular, intraperitoneal, subcutaneous and intravenous use, the coπpounds of the invention will generally be provided in sterile aqueous solutions or suspensions, buffered to an appropriate pH and isotonicity. Suitable aqueous vehicles include Ringer's solution and isotonic sodium chloride. Aqueous suspensions according to the invention may include suspending agents such as cellulose derivatives, sodium alginate, polyvinyl- pyrrolidone and gum tragacanth, and a wetting agent such as lecithin. Suitable preservatives for aqueous suspensions include ethyl and n-propyl p-hydroxybenzoate. Effective doses of the compounds of the present invention may be ascertained by conventional methods. The specific dosage level required for any particular patient will depend on a number of factors, including the severity of the condition being treated and the weight of the patient. In general, however, the daily dose (whether administered as a single dose or as divided doses) will be in the range 0.001 to 5000 mg per day, more usually from 1 to 1000 mg per day, and most usually from 10 to 200 mg per day. Expressed as dosage per unit body weight, a typical dose will be between 0.01 μg/kg and 50mg/kg, especially between 10 μg/kg and 10 mg/kg, eg. between 100 μg/kg and 2 mg/kg. The invention is now further illustrated by means of the following examples. Example 1 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (1-adamantanemethylamino- carbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 a. 2,3,5, 6-dibenzobicyclo[2.2.2]octane-7, 8-dicarboxylic acid anhydride Anthracene (8.9 g, 0.05 mol) and maleic anhydride (4.9 g, 0.05 mol) were refluxed for 3h in toluene (200 ml) . Upon cooling the title compound was obtained as white crystals which were isolated by filtration (10.2g, 74%). b. (,+)-cis-8- (l-adamantanemethylaminocarbonyl) -2,3,5, 6-dibenzo bicyclo[2.2.2]octane-7-carboxylic acid 2,3,5, 6-dibenzobicyclo(2.2.2.)octane-7, 8-dicarboxylic acid anhydride (prepared in step a) (276 mg, 1.0 mmol) and 1-adamantane- ethylamine (182 mg, 1.1 mmol) were dissolved in dry THF (5 ml) and refluxed for lh. A thick white precipitate was formed and this was isolated by filtration and washed with THF to leave the title compound (320 mg, 72%), m.p. 237-9°, found: C,78.76; H, 7.18; N, 3.33. C 29 H 31 N0 3 requires C, 78.88; H, 7.08; N, 3.17% c. 3,5-dibenzyloxycarbonyl-nitrobenzene 5-nitro-isophthalic acid (21.lg, 0.1 mol), thionyl chloride (80 ml) and DMF (10 drops) were stirred and heated for about lh until a clear solution was obtained. Excess thionyl chloride was removed by evaporation and the residual acid chloride was coevaporated with dichloromethane (2 x 100 ml) to remove the last traces. Benzyl alcohol (21.6 g, 0.2 mol) and triethylamine (30.03 g, 0.3 mol) were dissolved in dichloromethane (200 ml) and stirred at 0° under an atmosphere of dry nitrogen and a solution of the acid chloride in dichloromethane (50 ml) was added dropwise over 20 min. The solution was stirred and refluxed for lh, and the solution was cooled. The organic layer was washed with water (2 x 100ml), saturated sodium hydrogencarbonate solution (100 ml) and dried over magnesium sulphate. The solution was filtered and evaporated to leave the title compound (39.lg, 100%). d. 3,5-dibenzyloxycarbonyl-aniline The nitro compound prepared in step c above (3.91g, 10 mol) was dissolved in ethyl acetate (50 ml) and tin(II)chloride dihydrate (11.27g, 50 mmol) was added and the mixture stirred and heated at 70° under an atmosphere of nitrogen for lh. The mixture was poured carefully onto 5% sodium hydrogencarbonate solution (200 ml) and a further aliquot of ethyl acetate (100 ml) was added. After shaking the organic layer was separated and the aqueous layer was extracted with more ethyl acetate (50 ml) . The combined organic layers were washed with brine, and dried, filtered and evaporated to leave a pale yellow solid (3.25g, 90%) . e. N-tert-butyloxycarbonyl-lS- (3,5-dibenzyloxycarbonylphenylamino- carbonyl) -2-phenylethylamine BOC-L-phenylalanine (8.76 g, 33 mmol) was dissolved in dry dichloromethane (200 ml) and dry diisopropylethylamine (11.48 ml, 66 mmol) was added followed by PyBROP (15.33g, 33 mmol). The mixture was stirred at room temperature for 5 min and then the amine prepared in step d above (7.22 g, 20 mmol) was added. The solution was stirred at room temperature for a further 5h and the solution was then washed sequentially with 2M hydrochloric acid, water, saturated sodium hydrogencarbonate solution and water and finally dried, filtered and evaporated to leave an oil. This was purified by column chromatography (90% dichloromethane and 10% ethyl acetate) to leave the title compound as a white solid (11.0 g, 90%) f. cis-7- (IS- (3, 5-dibenzyloxycarbonylphenylaminocarbonyl) -2- phenylethylaminocarbonyl) -8- (1-adamantanemethylaminocarbonyl) -2, 3,5,6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 N-tert-butyloxycarbonyl-lS- (3, 5-dibenzyloxycarbonylphenylamino- carbonyl)-2-phenylethylamine prepared in step e above (8.0 g, 13 mmol) was dissolved in trifluoroacetic acid (40 ml) and stirred at room temperature for 30 min. The solvent was removed by evaporation and the residue taken up in dry dichloromethane (50 ml) and basified with diisopropylethylamine. Meanwhile (.+) -cis-8- (1-adamantanemethylaminocarbonyl)-2,3,5,6- dibenzo bicyclo[2.2.2]octane-7-carboxylic acid prepared in step b above (5.75 g, 13 mmol) was suspended in dry dichloromethane (150 ml) and diisopropylethylamine (4.6 ml, 26 mmol) was added followed by PyBOP (6.76 g, 13 mmol) . The solution was stirred until a clear solution was obtained and the solution of amine prepared above was added. After stirring at room temperature for 3h, the solution was washed sequentially with 2M hydrochloric acid and water, dried, filtered and evaporated. The residual oil was purified by column chromatography (90% dichloromethane and 10% ethyl acetate) to leave two compounds. The less polar material was designated the title compound (4.65 g) . g. cis-7-(IS-(3,5-dicarboxyphenylaminocarbonyl)-2-phenylethylamino- carbonyl) -8- (1-adamantanemethylaminocarbonyl) -2,3,5, 6-dibenzo- bicyclo[2.2.2]octane Diastereoisomer 1 The dibenzylester prepared in step f above (4.65g, 5.0 mmol) was dissolved in 1:1 methanolic THF (40 ml) . 10% palladium on charcoal (400 mg) was added and the reaction was stirred under an atmosphere of hydrogen overnight. The catalyst was removed by filtration through celite and the product isolated by evaporation (3.53 g) . The compound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 61.38; H, 7.54; N, 4.24. C 60 H 7 c,N 5 O 17 . 4.2 mol dioxan requires C, 60.99; H, 7.51; N, 4.63% Example 2 Preparation of cis-7-(IS-(3,5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl)-8- (1-adamantanemethylamino- carbonyl)-2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The compound was prepared essentially as in example 1 except that the more polar material from the chromatography described in step f was used in the final hydrogena ion. The compound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 64.34; H, 6.64; N, 4.70. C, 3 H 62 N - • 1-7 mol dioxan. 0.8 H : 0 requires C, 64.63; H, 7.00; N, 5.04% Example 3 Preparation of cis-7- (1R-(3,5-dicarboxphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (1-adamantanemetbylamino- carbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 The coπpound was prepared essentially as in example 1 except that BOC-D-phenylalanine was used in step e instead of BOC-L- phenylalanine. The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 65.66; H, 6.68; N, 5.46. C 53 H 62 N 4 0 12 . 1.3 H 2 0 requires C, 65.55; H, 6.71; N, 5.76% Example 4 Preparation of cis-7- (1R- (3,5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl)-8-(1-adamantanemethylamino- carbonyl)-2,3,5,6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The compound was prepared essentially as in exaπple 3 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was further characterised and tested as the N-metbyl- D-glucamine salt found: C, 64.77; H, 7.00; N, 5.90. C 53 H G2 N 4 0 12 . 2.0 H 2 0 requires C, 64.75; H, 6.76; N, 5.69% Example 5 Preparation of cis-7-(IS-(3,5-dicarboxyphenylamino- carbonyl) -2-cyclohexylethylaminocarbonyl) -8-(1-adamantanemethyl- aminocarbonyl) -2,3,5, 6- dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that BOC-L-2-cyclohexylalanine was used in step e instead of BOC-L- phenylalanine and no attempt was made to separate the diastereomers in step. The compound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 60.31; H, 8.00; N, 5.84. C 60 H εE N 5 O 17 . 2.5 H : 0 requires C, 60.38; H, 7.60; N, 5.86% Example 6 Preparation of cis-7- (IS- (3, 5-dicarboxphenylamino- carbonyl) -2- (2-thiophenyl) -ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The compound was prepared essentially as in exaπple 1 except that BOC-L-2-thiophenylalanine was used in step e instead of BOC-L- phenylalanine . The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 58.61; H, 6.43; N, 5.00. C^H j -oN.- .S . 5.0 H 2 0 requires C, 58.72; H, 6.76; N, 5.37% Example 7 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- ( 2 -thiophenyl) ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The compound was prepared essentially as in example 6 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 59.26; H, 6.66; N, 5.00. C 51 H 61; ,N 4 0 12 S . 4.5 H 2 0 requires C, 59.23; H, 6.73; N, 5.40% Example 8 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- ( -fluorophenyl) -ethylaminocarbonyl) -8- (1 -adamantane¬ methyl aminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The compound was prepared essentially as in example 1 except that BOC-L-4-fluorophenylalanine was used in step e instead of BOC-L- phenylalanine . The coπpound was further characterised and tested as the di-N- me thy 1 -D-glucamine salt found: C, 59.38; H, 7.19; N, 5.92. C 60 H 78 N 5 O 17 F. 3.1 H : 0 requires C, 59.24; H, 6.98; N, 5.76% Example 9 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- (4-f luorophenyl) ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 8 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- me thy 1 -D-glucamine salt found: C, 60.18; H, 7.21; N, 6.01. C 6 oH 78 N s O F . 2.3 H : 0 requires C, 59.94; H, 6.93; N, 5.83% Example 10 Preparation of cis-7- (IS- (3, 5-dicarboxphenylamino- carbonyl) -2- (4-chlorophenyl) -ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 except that BOC-L-4-chlorophenylalanine was used in step e instead of BOC-L- phenylalanine . The coπpound was further characterised and tested as the di-N- ethyl -D-glucamine salt found: C, 59.16; H, 7.04; N, 5.72. C 60 H 78 N 5 0 17 C1. 1.9 H_0 requires C, 59.49; H, 6.81; N, 5.78% Example 11 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- ( 4 -chlorophenyl) ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 10 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 59.08; H, 6.97; N, 5.43. C 60 H 78 Nr,O ]7 Cl . 2.7 H_0 requires C, 58.85; H, 6.86; N, 5.72% Example 12 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- (4-methoxyphenyl) -ethylaminocarbonyl) -8- (1 -adamant ane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 except that BOC-L-4-methoxyphenylalanine was used in step e instead of BOC-L- phenylalanine . The coπpound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 59.36; H, 7.28; N, 5.77. C 61 H 81 N 5 0 16 . 3.3 H ^ O requires C, 59.45; H, 7.17; N, 5.68% Example 13 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- (4-methoxphenyl) ethylaminocarbonyl) -8- ( 1 -adamantane¬ methyl ami noc a rbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2]octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 12 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 57.14; H, 7.26; N, 5.32. C 61 H 81 N s 0 18 . 6.3 H 2 0 requires C, 57.01; H, 7.34; N, 5.45% Example 14 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- (2-naphthyl) -ethylaminocarbonyl) -8- (1 -adamant ane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The compound was prepared essentially as in example 1 except that BOC-L-3- ( 2 -naphthyl )alanine was used in step e instead of BOC-L- phenylalanine . The coπpound was further characterised and tested as the di-N- ethyl -D-glucamine salt found: C, 61.14 H, 7.03; N, 5.64. C M H 81 N s 0 17 . 3.5 HD requires C, 61.20; H, 7.07; N, 5.58% Example 15 Preparation of cis-7-(IS- (3,5-dicarboxyphenylamino- carbonyl) -2- (2-naphthyl) ethylaminocarbonyl) -8- (1-adamantane¬ methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The compound was prepared essentially as in exaπple 14 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 61.59; H, 7.11; N, 5.52. C M H 8 ιN 5 0 17 . 4.2 H_0 requires C, 61.59; H, 7.11; N, 5.52% Example 16 Preparation of cis-7-(IS-(3,5-dicarboxyphenylamino- carbonyl) -2-(2-fluorophenyl) -ethylaminocarbonyl) -8-(1-adamantane- methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 except that BOC-L-2-fluorophenylalanine was used in step e instead of BOC-L- phenylalanine. The coπpound was further characterised and tested as the di-N- methy1-D-glucamine salt found: C, 58.63; H, 6.99; N, 5.49. C 60 H 78 N 5 O 17 F. 4.0 H_0 requires C, 58.44; H, 7.04; N, 5.68% Example 17 Preparation of cis-7- (IS- (3,5-dicarboxyphenylamino- carbonyl) -2- (2-fluorophenyl)ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl) -2 , 3 , 5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 16 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- metbyl -D-glucamine salt found : C, 58 .49 ; H, 7 . 00 ; N, 5 . 67 . C 60 H 78 Nr,O 17 F . 4 .2 H 0 requires C, 58 .34 ; H, 7 . 04 ; N, 5 . 67% Example 18 Preparation of cis-7-(IS- (3, 5-dicarboxyphenylamino- carbonyl) -2- (3-fluorophenyl) -ethylaminocarbonyl) -8-(1-adamantane¬ methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The compound was prepared essentially as in exaπple 1 except that BOC-L-3-fluorophenylalanine was used in step e instead of BOC-L- phenylalanine. The compound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 58.60; H, 7.04; N, 5.62. C 60 H 78 N 5 θ 17 F- 3.9 H-.0 requires C, 58.55; H, 7.038; N, 5.69% Example 19 Preparation of cis-7-(IS- (3,5-dicarboxyphenylamino- carbonyl) -2- (3-fluorophenyl)ethylaminocarbonyl) -8- (1-adamantane¬ methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The compound was prepared essentially as in exaπple 18 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 58.49; H, 7.09; N, 5.72. C 60 H 78 N 5 O π F. 3.9 H : 0 requires C, 58.544; H, 7.03; N, 5.69% Example 20 Preparation of cis-7-(IS- (3,5-dicarboxyphenylamino- carbonyl) -2-(2-chlorophenyl) -ethylaminocarbonyl)-8-(1-adamantane- ethylaminocarbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that BOC-L-2-chlorophenylalanine was used in step e instead of BOC-L- phenylalanine and no attempt was made to separate the diastereomers in step f. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 57.69; H, 7.05; N, 5.55. C 60 H 78 N 5 O 17 Cl . 4 .1 H 2 0 requires C, 57 .59 ; H, 6 .95 ; N, 5 .55% Example 21 Preparation of cis-7- (1R- (3, 5-dicarboxyphenylamino- carbonyl) -2- (2-thiophenyl)ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl) -2 , 3 , 5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 7 except that except that BOC-D-2-thiophenylalanine was used in step e instead of BOC-L-phenylalanine. The compound was further characterised and tested as the di-N- ethyl-D-glucamine salt Example 22 (comparative) Preparation of cis-7- (IS- (phenylammo¬ carbonyl) -2-phenyl-ethylaminocarbonyl) -8-(1-adamantanemethylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 step f except that N-tert-butyloxycarbonyl-lS- (phenylaminocarbonyl) -2-phenyl- ethylamine was used instead of N-tert-butyloxycarbonyl-lS- (3,5- dibenzyloxycarbonylphenylaminocarbony1) -2-phenylethylamine. As before, the less polar coπpound after chromatography was designated the coπpound of this example, found: C, 78.33; H, 6.94; N, 6.05 C 44 H 4 N 3 0 3 . 0.4 ethyl acetate requires C,78.49; H, 6.94; N, 6.05% Example 23 (comparative) Preparation of cis-7-(IS-(phenylamino¬ carbonyl) -2-phenylethylaminocarbonyl)-8- (1-adaπBntanemethylamino- carbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 22 except that the more polar material from the chromatography was designated as the compound of this example found: C, 79 .37 ; H, 6. 96 ; N, 6.24 C 44 H 45 N 3 0 3 requires C, 79 . 61 ; H, 6.83 ; N, 6.33% Example 24 Preparation of cis-7- (IS- (3 , 5 -dicarboxypheny lamino¬ carbonyl) -2- (4-hydroxyphenyl) -ethylaminocarbonyl ) -8- (1 -adamant ane- methylaminocarbonyl ) - 2 , 3 , 5 , 6 -dibenzobicyclo [ 2 . 2 . 2 ] octane. Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 except that BOC-L-tyrosine was used in step e instead of BOC-L-phenylalanine. The coπpound was further characterised and tested as the di-N- metbyl -D-glucamine salt Example 25 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl ) -2 - ( 4 -hydroxyphenyl ) ethylaminocarbonyl ) - 8 - ( 1 -ada antane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The coπpound was prepared essentially as in example 24 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- ethyl -D-glucamine salt found: C, 57.11; H, 7.46; N, 5.79. C 60 H 79 Nc,O :8 . 6.0 H 2 0 requires C, 56.95; H, 7.24; N, 5.53% Example 26 Preparation of cis-7- (1R- (3, 5-dicarboxyphenylamino- carbonyl) -2- (4 -hydroxyphenyl) -ethylaminocarbonyl) -8- (1-adamantane- methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 except that BOC-D-tyrosine was used in step e instead of BOC-L-phenylalanine. The coπpound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 57.05; H, 7.09; N, 5.23. C 60 H 79 N 5 O 18 . 6.1 H 2 0 requires C, 56.79; H, 7.25; N, 5.52% Example 27 Preparation of cis-7- (1R- (3, 5-dicarboxyphenylamino- carbonyl) -2- ( 4 -hydroxyphenyl) ethylaminocarbonyl) -8- (1 -adamant ane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The compound was prepared essentially as in exaπple 27 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. Example 28 Preparation of cis-7- (IS- (3,5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (1-cycloheptane- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The compound was prepared essentially as in exaπple 1 except that cycloheptanemethylamine was used in step b instead of 1 -adamantane¬ methyl amine . The coπpound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 59.25; H, 7.17; N, 5.94. C 57 H 77 N 5 0 17 . 2.9 H_0 requires C, 59.14; H, 7.22; N, 6.06% Example 29 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (cycloheptanemethyl- a i no carbonyl ) -2 , 3 , 5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The compound was prepared essentially as in example 28 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 59.14; H, 7.15; N, 6.10. C 57 H 77 N 5 0 17 . 2.9 H 2 0 requires C, 59.14; H, 7.22; N, 6.06% Example 30 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-pherylethylaminocarbonyl) -8- (1-cyclohexanemethylamino- carbonyl)-2,3, 5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 The coπpound was prepared essentially as in example 1 except that cyclohexanemethylamine was used in step b instead of 1-adamantane- metbylamine. The coπpound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 58.75 ; H, 7 .11 ; N, 5.85. C 56 H 75 N 5 0 17 . 3 .2 H 2 0 requires C, 58 .58 ; H, 7 .15 ; N, 6 .10% Example 31 Preparation of cis-7- (IS-(3,5-dicarboxyphenylamino¬ carbonyl) -2-phenylethylaminocarbonyl) -8-(cyclohexanemethylamino- carbonyl)-2,3,5,6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 30 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 58.61; H, 7.19; N, 6.18. C 56 H 75 N £ 0 17 . 3.1 H.0 requires C, 58.69; H, 7.14; N, 6.11% Example 32 Preparation of cis-7-(IS-(3,5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl)-8-(1-naphthalenemethylamino- carbonyl)-2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 The coπpound was prepared essentially as in exaπple 1 except that 1-naphthalenemethylamine was used in step b instead of 1- adamantanemethylamine. The coπpound was further characterised and tested as the di-N- metbyl-D-glucamine salt found: C, 59.82; H, 6.70; N, 6.04. C 60 H 71 N 5 O 17 . 3.7 H 2 0 requires C, 60.03; H, 6.58; N, 5.83% Example 33 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino¬ carbonyl)-2-phenylethylaminocarbonyl)-8- (1-naphthalenemethylamino- carbonyl)-2,3, 5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 32 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 58.02; H, 6.84; N, 5.96. C 60 H 71 N 5 O 17 . 5.6 H 2 0 requires C, 58.31; H, 6.71; N, 5.67% Example 34 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (3, 4-dichlorophenyl- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The coπpound was prepared essentially as in example 1 except that 3,4-dichlorobenzylamine was used in step b instead of 1- adamantanemethylamine . The coπpound was further characterised and tested as the di-N- metbyl -D-glucamine salt found: C, 56.58; H, 6.27; N, 5.90. C 56 H 67 C1 2 N 5 0 17 . 1.9 H.0 requires C, 56.65; H, 6.01; N, 5.89% Example 35 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (3, 4-dichlorophenyl- methylaminocarbonyl ) -2 , 3 , 5 , 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 2 The coπpound was prepared essentially as in example 34 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was further characterised and tested as the di-N- methyl -D-glucamine salt found: C, 56.98; H, 6.04; N, 6.05. C 56 H 67 Cl 2 N 5 O π . 1.4 H 2 0 requires C, 57.08; H, 5.97; N, 5.94% Example 36 Preparation of cis-7-(IS- (3,5-dicarboxyphenylamino- carbonyl) -3-phenylpropylaminocarbonyl) -8- (1-adamantanemethyl- aminocarbonyl) -2,3,5,6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that 2S- (tert-butyloxycarbonylamino) -4-phenylbutanoic acid was used in step e instead of BOC-L-phenylalanine and no attempt was made to separate the diastereomers in step f. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 59.76; H, 7.03; N, 5.79. C 51 H 81 N 5 0 17 . 3.6 H 2 0 requires C, 60.01; H, 7.28; N, 5.74% Example 37 Preparation of cis-7- (1R- (3,5-dicarboxyphenylamino- carbonyl)-3-phenylpropylaminocarbonyl)-8-(1-adamantanemethylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The compound was prepared essentially as in exaπple 1 except that 2R- (tert-butyloxycarbonylamino)-4-phenylbutanoic acid was used in step e instead of BOC-L-phenylalanine and no attempt was made to separate the diastereomers in step f. The compound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 59.65; H, 7.01; N, 5.51. C 61 H 81 N 5 0 17 . 3.7 H 2 0 requires C, 59.93; H, 7.28; N, 5.73% Example 38 Preparation of cis-7- (IS- (3,5-dicarboxyphenylamino- carboπyl)-2-phenylethyl-N (methyl) -aminocarbonyl)-8- (1-adamantane- methylaminocarbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that N-metbyl-BOC-L-phenylalanine was used in step e instead of BOC-L- phenylalanine and no attempt was made to separate the diastereomers in step f. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 58.78; H, 7.09; N, 5.70. C 61 H 81 N ; A 7 - 4.7 H : 0 requires C, 59.04; H, 7.34; N, 5.64% Example 39 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -3-phenylmethylaminocarbonyl) -8- (1-adamantanemethyl- aminocarbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that BOC-L-phenylglycine was used in step e instead of BOC-L-phenyl¬ alanine and no attempt was made to separate the diastereomers in step f. The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 64.20; H, 6.75; N, 5.80. C 52 H 60 N 4 O 12 . 2.2 H 2 0 requires C, 64.21; H, 6.67; N, 5.76% Example 40 Preparation of cis-7-(IS-(3-carboxyphenylaminocarbonyl)- 2-phenylethylaminocarbonyl) -8- (1-adamantanemethylaminocarbonyl)- 2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 The coπpound was prepared essentially as in example 1 steps f and g except that N-tert-butyloxycarbonyl-lS- (3-benzyloxycarbonyl- phenylaminocarbonyl)-2-phenylethylamine was used instead of N-tert- butyloxycarbonyl-lS-(3,5-dibenzyloxycarbonyl-phenyl aminocarbonyl)- 2-phenylethylamine in step f. As before, the less polar coπpound after chromatography in step f was taken through to the coπpound of this exaπple. The compound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 69.70; H, 7.00; N, 5.96. C 5 .H ι .N 4 0 1(1 . 0.7 H 2 0 requires C, 69.45; H, 7.10; N, 6.23% Example 41 Preparation of cis-7-(IS-(3-carboxyphenylaminocarbonyl)- 2-phenylethylaminocarbonyl)-8- (1-adamantanemethylaminocarbonyl)- 2,3,5,6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The coπpound was prepared essentially as in exaπple 40 except that the more polar. The compound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 66.60; H, 7.21; N, 6.06. C 5 H ϋ N 4 O 10 . 1.9 H 2 0 requires C, 66.57; H, 7.08; N, 5.97% Example 42 Preparation of cis-7- (IS- (3-carboxy-5-methoxycarbonyl- phenylaminocarbonyl) -2-phenylethylaminocarbonyl)-8-(1-adamantane- methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The compound was prepared essentially as in exaπple 1 except that 3-methoxycarbonyl-5-nitrobenzoic acid was used in step c instead of 5-nitro-isophthalic acid. The compound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 65.81; H, 6.93; N, 5.79. C^H^NA ; ,. 1.5 H 2 0 requires C, 65.70; H, 6.83; N, 5.67% Example 43 Preparation of cis-7-(IS- (3,4-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (1-adamantanemethyl- aminocarbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The corrpound was prepared essentially as in exarrple 1 except that 4-nitrophthalic acid was used in step c instead of 5-nitro- isophthalic acid. The coπpound was further characterised and tested as the di-N- methyl-D-glucamine salt found: C, 60.31; H, 7.22; N, 5.76. C 60 H 79 N 5 O 17 . 1.5 HO requires C, 60.29; H, 7.16; N, 5.86% Example 44 Preparation of cis-7- (IS-(3,5-ditetrazolylphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (1-adamantanemethyl- aminocarbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The corrpound was prepared essentially as in exarrple 1 except that the bis pivaloyloxymethyl (POM) derivative of N-tert-butyloxy- carbonyl-lS- (3, 5-ditetrazolylphenylaminocarbonyl) -2-phenylethyl- amine was used instead of N-tert-butyloxycarbonyl-lS- (3,5- dibenzyloxycarbonylphenylaminocarbonyl) -2-phenylethylamine in step f and the deprotection in step g was performed with methanolic ammonia solution. Example 45 (comparative) Preparation of cis-7- (IS- (2-carboxy- phenylaminocarbony1) -2-phenylethylaminocarbonyl) -8-(1-adamantane- methylaminocarbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The corrpound was prepared essentially as in exaπple 1 steps f and g except that N-tert-butyloxycarbonyl-lS- (2-benzyloxycarbonyl- phenyla inocarbonyl)-2-phenylethylammewas used instead of N-tert- butyloxycarbonyl-lS-(3,5-dibenzyloxycarbonylphenylaminocarbonyl)-2- phenylethylamine in step f. No attempt was made to separate diastereoisomers. The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 63.51; H, 7.00; N, 5.52, C c , 2 H t2 4 0 j „. 4.2 H 2 0 requires C, 63.77; H, 7.25; N, 5.72%. Example 46 (comparative) Preparation of cis-7- (IS- (4-carboxy- phenylaminocarbonyl ) -2-phenylethylaminocarbonyl) -8- (1 -adamantane¬ methyl aminocarbonyl ) - 2 , 3 , 5 , 6 -dibenzobicyclo [ 2 . 2 .2 ] octane Diastereomer 1 The coπpound was prepared essentially as in exaπple 1 steps f and g except that N-tert-butyloxycarbonyl-lS- (2-benzyloxycarbonyl- phenyla inocarbonyl ) -2-phenylethylamine was used instead of N-tert- butyloxycarbonyl-lS- (3 , 5-dibenzyloxycarbonylphenylaminocarbonyl) -2- phenylethylamine in step f . The less polar material described from the chromatography in step f was taken through to the title coπpound of this example . The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 66.60 ; H, 7 .21 ; N, 6.06. C 52 H ώ N 4 0 lfl . 1.9 H 2 0 requires C, 66.57 ; H, 7 .08 ; N, 5.97% . Example 47 (comparative) Preparation of cis-7- (IS- (4-carboxy- phenylaminocarbonyl ) -2 -phenylaminocarbonyl-8- ( 1 -adamantane- methylaminocarbonyl ) - 2 , 3 , 5 , 6 -dibenzobicyclo [ 2 . 2 . 2 ] octane Diastereomer 2 The more polar diastereomer described in example 46 was taken through as the title compound of this exarrple. The corrpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 62.98; H, 7.26; N, 6.02. C S2 H 62 N 4 O 10 4.6 H 2 0 requires C, 63.32; H, 7.28; N, 5.68%. Example 48 (comparative) Preparation of cis-7-(IS- (3,5-dimethox - carbonylphenylaminocarbonyl) -2-phenylethylamino-carbonyl-8- (1- adamantanemethylaminocarbonyl)-2,3,5,6-dibenzobicyclo[2.2.2]octane Diastereomer 1 The coπpound of exaπple 1 was treated with an excess of diazomethane to leave the title coπpound after quenching and evaporation found: C, 74.00; H, 6.40; N, 5.32. C 4t) H 4P N 3 0 7 requires C, 73.92; H, 6.33; N, 5.39% Example 49 Preparation of cis-7-(IS- (3,5-dicarboxyphenylamino- carbonyl)-2-phenylethylaminocarbonyl)-8-(1-(1-adamantane)-1-methyl- ethylaminocarbonyl)-2,3,5,6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The corrpound was prepared essentially as in exarrple 1 except that 1- (1-adamantane) -1-methylethylamine was used in step b instead of 1-adamantanemethylamine and no attempt was made to separate the diastereomers described in step f. m.p 195-8°. The corrpound was further characterised and tested as the di N- ethyl-D-glucamine salt found: C, 58.96; H, 7.33; N, 5.59. C 62 H 83 N ς O π - 5 H 2 0 requires C, 659.08; H, 7.43; N, 5.56% Example 50 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (3-indolylethylamino- carbonyl)-2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 1 The corrpound was prepared essentially as in exaπple 1 except that 3-indolylethylamine was used in step b instead of 1-adamantane- methylamine. The coπpound was further characterised and tested as the di N- methyl-D-glucamine salt found: C, 58.37; H, 6.44; N, 7.04. C 59 H 72 N 6 0 17 . 4 H 2 0 requires C, 58.60; H, 6.67; N, 5.95% Example 51 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (3-indolylethylamino- carbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The corrpound was prepared essentially as in exaπple 50 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The corrpound was further characterised and tested as the di N- methyl-D-glucamine salt found: C, 59.83; H, 6.20; N, 6.76. C 59 H 72 N b O π - 2.4 HO requires C, 60.03; H, 6.56; N, 7.12% Example 52 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl)-8- (2-thiophenylmethylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that 2-thiophenylmethylethylamine was used in step b instead of 1- adamantanemethylamine and no attempt was made to separate the diastereomers described in step f. The coπpound was further characterised and tested as the di N- methyl-D-glucamine salt found: C, 52.68; H, 6.43; N, 5.67. C 54 H 67 N b 0 17 S. 7.4 H_0 requires C, 52.99; H, 6.74; N, 5.72% Example 53 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl) -8- (2-phenylethylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exarrple 1 except that 2-phenylethylamme was used in step b instead of 1-adamantane- methylamine and no atteπpt was made to separate the diastereomers described in step f. The coπpound was further characterised and tested as the di N- ethyl-D-glucamine salt found: C, 59.15; H, 6.83; N, 5.81. C 57 H 7 ιN 5 O . 3.5 H 2 0 requires C, 58.95; H, 6.77; N, 6.03% Example 54 Preparation of cis-7- (IS-(3,5-dicarboxyphenylamino¬ carbonyl) -2-phenylethylaminocarbonyl) -8-(2-phenylpropylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane (mixture of diastereomers) The coπpound was prepared essentially as in exaπple 1 except that 3-phenylpropylamine was used in step b instead of 1-adamantane- metbylamine and no atteπpt was made to separate the diastereomers described in step f. The corrpound was further characterised and tested as the di di¬ methyl-D-glucamine salt found: C, 59.39; H, 6.79; N, 5.90. C 58 H 73 N s O • 3.8 H 2 0 requires C, 59.38; H, 6.85; N, 5.96% Example 55 Preparation of cis-7-(IS-(3-carboxyphenylaminocarbonyl)- 2- (2-thiophenyl)ethylaminocarbonyl) -8- (1-adamantanemethylamino- carbonyl) -2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereomer 1 The coπpound was prepared essentially as in example 1 except that step e was carried out using BOC-L-3- (2-thiophenyl)alanine instead of BOC-L-phenylalanine and 3-benzyloxycarbonylaniline instead of 3,5-dibenzyloxycarbonylaniline. The product of this reaction was used in step f instead of N-tert-butlyloxycarbonyl-lS- (3,5- dibenzyloxycarbonylphenylaminocarbonyl) -2-phenylethylamme. The coπpound was further characterised and tested as the N-metbyl- D-glucamine salt found: C, 66.21; H, 6.62; N, 5.87. Cr, 0 H t , 0 4 O 10 S requires C, 66.06; H, 6.65; N, 6.16% Example 56 Preparation of cis-7- (IS- (3-carboxyphenylaminocarbonyl) - 2- (2-thiophenyl ) ethylaminocarbonyl ) -8- ( 1-adamantanemethylamino- carbonyl) -2 , 3 , 5 , 6-dibenzobicyclo [2.2 .2 ] octane Diastereomer 2 The corrpound was prepared essentially as in exaπple 55 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 66.32; H, 6.81; N, 5.88. C 50 H bC N 4 O 10 S requires C, 66.06; H, 6.65; N, 6.16% Example 57 Preparation of cis-7-(IS-(3-carbo-xypheπylaminocarbonyl)- 2- (2-thiophenyl)ethylaminocarbonyl) -8- (1-naphthalenemethylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane Diastereomer 1 The coπpound was prepared essentially as in exarrple 55 except that 1-naphthalenemethylamine was used in step b instead of 1- adamantanemethylamine. The corrpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 58.71; H, 6.21; N, 5.85. C r ,,,Hr, 2 N 4 0 Kl S. 6.4 H 2 0 requires C, 59.06; H, 6.43; N, 5.51% Example 58 Preparation of cis-7-(IS-(3-carboxyphenylaminocarbonyl)- 2- (2-thiophenyl)ethylaminocarbonyl)-8- (1-naphthalenemethylamino- carbonyl) -2,3, 5, 6- dibenzobicyclo[2.2.2]octane Diastereomer 2 The coπpound was prepared essentially as in exarrple 57 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The corrpound was tested as the N-methyl-D-glucamine salt. Example 59 Preparation of cis-7-(IS-(3-carboxyphenylaminocarbonyl)- 2-phenylethylaminocarbonyl)-8- (1-naphthaienemethylaminocarbonyl) - 2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereomer 1 The coπpound was prepared essentially as in exarrple 57 except that BOC-L-phenylalanine was used in step e instead of BOC-L-3-(2- thiophenyl)alanine. The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 58.71; H, 6.21; N, 5.85. C 50 H £2 N 4 O 10 S. 6.4 H 2 0 requires C, 59.06; H, 6.43; N, 5.51% Example 60 Preparation of cis-7-(IS-(3-carboxyphenyla-πdnocarbonyl)- 2-phenylethylaminocarbonyl)-8- (1-naphthalenemethylaminocarbonyl) - 2,3,5, 6-dibenzobicyclo[2.2.2]octane Diastereomer 2 The compound was prepared essentially as in exaπple 59 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The compound was tested as the N-methyl-D-glucamine salt. Example 61 Preparation of cis-7- (IS- (3,5-dicarboxyphenylmethyl- aminocarbonyl) -2-phenylethylaminocarbonyl) -8- (1-adamantane¬ methylaminocarbonyl) -2,3, 5, 6-dibenzobicyclo [2.2.2] octane Diastereoisomer 1 The corrpound was prepared essentially as in exarrple 1 except that 3,5-dibenzyloxycarbonylbenzylamine was used in step e instead of 3, 5-dibenzyloxycarbonylaniline. The corrpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 64.72; H, 6.96; N, 5.88. Cr, 4 R, 4 N 4 0 12 . 2 H 2 0 requires C, 64.99; H, 6.88; N, 5.61% Example 62 Preparation of cis-7- (IS- (3, 5-dicarboxyphenylmethyl- aminocarbonyl) -2-phenylethylaminocarbonyl) -8- (1-adamantanemethyl- aminocarbonyl)-2,3, 5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The compound was prepared essentially as in exaπple 61 except that the more polar material from the chromatography described in step f was used in the final hydrogenation. The coπpound was further characterised and tested as the N-methyl- D-glucamine salt found: C, 64.99; H, 6.95; N, 5.65. C, 4 H,- 4 N 4 0 12 . 2 H 2 0 requires C, 64.99; H, 6.88; N, 5.61% Example 63 Preparation of cis-7- (IS- (3,5-dicarboxyphenylamino- carbonyl) -2-pheπylethylaminocarbonyl)-8- (2-naphthalenemethylamino- carbonyl) -2,3,5,6-dibenzobicyclo [2 .2.2]octane Diastereoisomer 1 • The corrpound was prepared essentially as in exarrple 1 except that 2-naphthalenemethylamine was used in step b instead of 1- adamantanemethylamine, found: C, 73.99; H, 5.26; N, 5.41. C 46 H 37 N 3 0 7 .requires C, 74.28; H, 5.01; N, 5.65% The corrpound was tested as the di N-methyl-D-glucamine salt. Example 64 Preparation of cis-7-(IS- (3, 5-dicarboxyphenylamino- carbonyl) -2-phenylethylaminocarbonyl)-8- (2-naphthalenemethylamino- carbonyl) -2,3, 5, 6-dibenzobicyclo[2.2.2]octane Diastereoisomer 2 The coπpound was prepared essentially as in exarrple 63 except that the more polar material from the chromatography described in step f was used in the final hydrogenation found: C, 72.26; H, 5.18; N, 5.42. C 4t .H 37 N 3 0 7 . 1.1 H.0 requires C, 72.30; H, 5.18; N, 5.50% The corrpound was tested as the di N-methyl-D-glucamine salt. The compounds of the examples gave the following ] H NMR spectra: Ex.la (d 6 -DMSO) δ 7.5 (2H, m) , 7.3 (2H, m) , 7.2 (4H, m) , 4.8 (2H, s) , 3.6 (2H,s) Ex.lb (d 7 -DMF) δ 7.7 (1H, t) , 7.4 (3H, m) , 7.2 (3H,m) , 7.1 (2H, ) , 4.7 (1H, d), 4.6 (1H, d) , 3.5 (1H, dd) , 3.0 (1H, dd) , 2.9 (1H, dd) , 2.7 (1H, dd) , 2.0 (3H, s) , 1.7 (6H, ) , 1.5 (6H, s) Ex.lc (CDC1 3 ) δ 9.0 (3H, d) , 7.5 (10H, m) , 5.5 (4H, s) Ex.Id (CDC1- δ 8.1 (1H, d) , 7.5 (12H, m) , 5.4 (4H, s) , 3.8 (2H, bs) Ex.le (d 6 -DMSO) δ 10.5 (IH, s) , 8.5 (2H, s) , 8.2 (IH, s) , 7.3 (15H, m) , 5.4 (4H, s), 4.3 (IH, m) , 2.9 (2H, m) , 1.3 (9H,s Ex.lg (d 6 -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s) , 8.0 (IH, d), 7.1 (14H, m) , 4.5 (2H, s) , 4.2 (IH, s) , 3.1 (2H, m) , 2.8 (2H, m), 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, s) Ex.2 (d 6 -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (15H, ), 4.4 (3H, m) , 3.1 (2H, ) , 2.8 (2H, m),2.6 (IH, m) , 2.2 (IH, m), 1.8 (3H, s), 1.5 (6H, m) , 1.2 (6H, s) Ex.3 (d b -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s) , 8.0 (IH, d) , 7.1 (14H, m) , 4.5 (2H, s) , 4.2 (IH, s) , 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, s) Ex.4 (d έ -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (15H, m) , 4.4 (3H, m) , 3.1 (2H, ) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.8 (3H, s) , 1.5 (6H, ) , 1.2 (6H, s) Ex.5 (d 6 -DMSO) δ 10.1 and 9.8 (IH, 2 x s) , 8.4 (2H, d) , 8.1 (IH, m) , 7.7 and 7.4 (IH, 2 x m) , 7.1 (9H, m) , 4.5 (2H, m) , 4.2 (IH, m) , 3.2 (2H, ) , 2.9 (IH, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.8-0.8 (28H, m) Ex.6 (d 6 -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (2H, s) , 7.2 (12H, m) , 4.4 (3H, m) , 3.0 (4H, ) , 2.6 (IH, ) , 2.2 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, s) Ex.7 (d b -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (13H, m) , 4.5 (3H, m) , 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m), 1.8 (3H, s) , 1.6 (6H, m) , 1.2 (6H, m) Ex.8 (d b -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s) , 8.0 (IH, d) , 7.2 (13H, m) , 4.5 (2H, m) , 4.2 (IH, s) , 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, ) , 2.2 (IH, ) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, s) Ex.9 (d 6 -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (14H, ) , 4.5 (3H, m) , 3.1 (IH, m) , 2.8 (3H, m) , 2.4 (2H, m) , 1.8 (3H, s) , 1.5 (6H, m) , 1.2 (6H, m) Ex.10 (d 6 -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s) , 8.0 (IH, d) , 7.2 (13H, m) , 4.5 (2H, m) , 4.2 (IH, ) , 3.1 (2H, m) , 3.1 (2H, m), 2.6 (IH, m), 2.2 (IH, ) , 1.7 (3H, s) , 1.5 (6H, ) , 1.1 (6H, s) Ex.11 (d 6 -DMSO) δ 9.9 (IH, ε) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (14H, m) , 4.5 (3H, m) , 3.1 (IH, m) , 2.9 (3H, m) , 2.4 (2H, m) , 1.8 (3H, s) , 1.5 (6H, m) , 1.2 (6H, m) Ex.12 (d 6 -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s) , 7.9 (IH, d) , 7.2 (13H, m) , 4.4 (2H, m) , 4.2 (IH, ) , 3.7 (3H, s) , 3.0 (4H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, s) Ex.13 (d b -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (14H, ) , 4.4 (3H, m) , 3.7 (3H, s) , 3.1 (2H, ) , 2.9(2H, m) , 2.4 (2H, m) , 1.8 (3H, s) , 1.6 (6H, m) , 1.2 (6H, m) Ex.14 (d b -DMSO) δ 13.2 (2H, br s) , 9.9 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s), 8.0 (IH, d) , 7.9-6.8 (16H, m) , 4.5 (2H, m) , 4.0 (IH, s) , 3.1 (2H, ) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, s) Ex.15 (d b -DMSO) δ 13.1 (2H, br s) , 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s), 7.9-6.8 (17H, m) , 4.5 (3H, m) , 3.1 (IH, m) , 2.8 (3H, m) , 2.4 (2H, m), 1.8 (3H, s) , 1.5 (6H, m) , 1.2 (6H, m) Ex.16 (d e -DMSO) δ 13.2 (2H, br s) , 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s), 8.0 (IH, d) , 7.2 (13H, m) , 4.5 (2H, m) , 4.2 (IH, s) , 3.1 (2H, ) , 2.8 (2H, m) , 2.6 (IH, ) , 2.2 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m), 1.1 (6H, s) Ex.17 (d-DMSO) δ 13.2 (2H, br s) , 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s), 7.1 (14H, m) , 4.5 (3H, m) , 3.1 (IH, m) , 2.8 (3H, m) , 2.4 (2H, m) , 1.8 (3H, s) , 1.5 (6H, ) , 1.2 (6H, m) Ex.18 (d 6 -DMSO) δ 13.2 (2H, br s) , 9.8 (IH, s) , 8.4 (2H, ε) , 8.1 (IH, ε), 8.0 (IH, d) , 7.2 (13H, m) , 4.5 (2H, m) , 4.2 (IH, ε) , 3.1 (2H, ), 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, ε) , 1.5 (6H, m) , 1.1 (6H, s) Ex.19 (d 6 -DMSO) δ 13.2 (2H, br s) , 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (14H, m) , 4.5 (3H, m) , 3.1 (IH, m) , 2.8 (3H, m) , 2.4 (2H, m) , 1.8 (3H, s) , 1.5 (6H, m) , 1.2 (6H, m) Ex.20 (d-DMSO) δ 13.2 (2H, br s) , 11.1 and 9.8 (IH, 2 x s) , 8.2 (4H, m) , 7.2 (13H, ) , 4.5-4.2 (3H, ) , 3.2-2.4 (6H, m) , 1.7-1.1 (15H, m) Ex.21 (d 6 -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, ε) , 7.1 (13H, m) , 4.5 (3H, m) , 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.8 (3H, s), 1.6 (6H, m) , 1.2 (6H, m) Ex.22 (CDClj) δ 8.6 (IH, s) , 7.6 (2H, d) , 7.2 (16H, m) , 5.8 (IH, m) , 5.6 (IH, m) , 4.8 (IH, m) , 4.5 (IH, d) , 4.2 (IH, d) , 3.2 (3H, m) , 3.0 (IH, dd) , 2.6 (IH, m) , 2.4 (IH, m) , 1.9 (3H, s) , 1.6 (6H, ) , 1.2 (6H, s) Ex.23 (CDCI 3 ) δ 8.8 (IH, s) , 7.3 (18H, m) , 6.1 (IH, m) , 6.0 (IH, m) , 5.0 (IH, m) , 4.4 (IH, d) , 4.1 (IH, d) , 3.7 (IH, dd) , 3.2 (IH, m) , 3.1-2.8 (3H, ) , 2.4 (IH, m) , 2.0 (3H, s) , 1.7 (6H, m) , 1.3 (6H, s) Ex.24 (d 6 -DMΞO) δ 9.8 (IH, s),9.2 (IH, br s) , 8.4 (2H, ε) , 8.1 (IH, s), 8.0 (IH, d) , 7.2 (11H, m) , 6.6 (2H, d) , 4.5 (IH, s) , 4.4 (IH, m) , 4.2 (IH, ε), 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, ε), 1.5 (6H, m) , 1.1 (6H, ε) Ex.25 (d b -DMSO) δ 9.8 (IH, s) , 9.2 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s), 7.1 (12H, ) , 6.6 (2H, d) , 4.4 (3H, m) , 3.1 (IH, m) , 2.8 (3H, m) , 2.4 (2H, m) , 1.8 (3H, s) , 1.5 (6H, m) , 1.2 (6H, m) Ex.26 (d 6 -DMSO) δ 9.8 (IH, s),9.2 (IH, br s) , 8.4 (2H, s) , 8.1 (IH, s), 8.0 (IH, d) , 7.2 (11H, m) , 6.6 (2H, d) , 4.5 (IH, s) , 4.4 (IH, ) , 4.2 (IH, s), 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, ) , 1.7 (3H, s), 1.5 (6H, m) , 1.1 (6H, s) Ex.27 (d 6 -DMSO) δ 9.8 (IH, s),9.2 (IH, br s) , 8.4 (2H, s) , 8.1 (IH, s), 8.0 (IH, d) , 7.2 (11H, m) , 6.6 (2H, d) , 4.5 (IH, s) , 4.4 (IH, m) , 4.2 (IH, s), 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (IH, ) , 2.2 (IH, ) , 1.7 (3H, s), 1.5 (6H, m) , 1.1 (6H, ε) Ex.28 (d b -DMSO) δ 9.8 (IH, s) , 8.4 (2H, s) , 8.1 (IH, s) , 8.0 (IH, d) , 7.1 (14H, m) , 4.5 (2H, m) , 4.2 (IH, s) , 3.1 (3H, m) , 2.7 (3H, ) , 1.4-0.7 (13H, m) Ex.29 (d e -DMSO) δ 9.9 (IH, s) , 8.3 (2H, s) , 8.1 (IH, s) , 7.1 (15H, m) , 4.5 (3H, m) , 3.1 (4H, m) , 2.6 (2H, m) , 1.4-0.7 (13H, m) Ex.30 (d b -DMSO) δ 9.8 (IH, s) , 8.4 (2H, ε) , 8.1 (IH, s) , 8.0 (IH, d) , 7.1 (14H, m) , 4.5 (2H, m) , 4.2 (IH, s) , 3.1 (3H, m) , 2.7 (3H, m) , 1.4-0.7 (11H, m) Ex.31 (d b -DMSO) δ 9.9 (IH, s) , 8.3 (2H, ε) , 8.1 (IH, ε) , 7.1 (15H, m) , 4.5 (3H, m) , 3.0 (4H, m) , 2.6 (2H, m) , 1.6-0.6 (11H, ) Ex.32 (d c -DMSO) δ 13.1 (2H, br s) , 9.8 (IH, s) , 8.4 (2H, ε) , 8.3- 6.8 (23H, m) , 4.7-4.2 (3H, m) , 3.2 (4H, m) , 2.7 (2H, m) Ex.33 (d 6 -DMSO) δ 13.2 (2H, br ε) , 10.0 (IH, ε) , 8.3 (2H, ε) , 8.2- 6.8 (23H, m) , 4.5 (3H, m) , 3.2 (3H, m) , 2.7 (3H, ) Ex.34 (d b -DMSO) δ 9.6 (IH, s) , 8.4-6.8 (21H, m) , 4.7-4.1 (3H, m) , 3.2 (4H, m) , 2.8 (2H, m) Ex.35 (d"-DMSO) δ 9.8 (IH, s) , 8.3-6.7 (21H, m) , 4.5-4.1 (3H, m) , 3.2 (4H, m) , 2.9 (2H, m) Ex.36 (d'-DMSO) δ 13.1 (2H, br s) , 10.1-9.7 (IH, 3 x ε) , 8.4-7.0 (18H, ) , 4.5-4.1 (3H, m) , 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (2H, m) , 2.0 (2H, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.1 (6H, m) Ex.37 (d 6 -DMSO) δ 13.1 (2H, br s) , 10.1-9.7 (IH, 3 x s) , 8.4-7.0 (18H, m) , 4.5-4.1 (3H, m) , 3.1 (2H, m) , 2.8 (2H, m) , 2.6 (2H, m) , 2.0 (2H, m) , 1.7 (3H, ε) , 1.5 (6H, m) , 1.1 (6H, m) Ex.38 (d 6 -DMSO) δ 13.2 (2H, br ε) , 11.0 and 10.9 (IH, 2 x ε) , 9.9 and 9.7 (IH, 2 x s) , 8.4-6.8 (15H, m) , 6.5 and 6.3 (IH, 2 x t) , 4.5-4.1 (3H, m) , 3.2 (5H, m) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, ) , 1.7 (3H, m) , 1.5 (6H, m) , 1.1 (6H, m) Ex.39 (d fr -DMSO) δ 10.5 and 10.3 (IH, 2 x s) , 8.4-8.0 (3H, m) , 7.2 (13H, m), 5.3 (IH, d) , 4.5 (2H, m) , 3.1 (2H, m) , 2.5 (2H, m) , 1.8 (3H, m) , 1.5 (6H, m) , 1.2 (6H, m) Ex.40 (d fe -DMSO) δ 9.7 (IH, s) , 8.2 (IH, ε) , 8.0 (IH, ), 7.8-6.9 (17H, m) , 4.5 (IH, s) , 4.4 (IH, s) , 4.2 (IH, s) , 3.5-3.2 (2H, ) , 2.9 (2H, m) , 2.6 (IH, m) , 2.3 (IH, m) , 1.7 (3H, s) , 1.5 (6H, m) , 1.4 (6H, ε) Ex.41 (d-DMSO) δ 12.9 (IH, s) , 9.7 (IH, s) , 8.2 (IH, s) , 7.6 (2H, t), 7.4-7.2 (11H, m) , 7.1 (2H, m) , 7.0 (2H, q) , 6.8 (IH, t) , 4.5 " (3H, m), 3.2 (IH, dd) , 3.0 (2H, m) , 2.9 (IH, dd) , 2.6 (IH, dd) , 2.4 (IH, dd) , 1.8 (3H, s) , 1.6 (6H, m) , 1.3 (6H, s) Ex.42 (d b -DMSO) δ 9.2-7.7 (5H, m) , 7.3 (13H, m) , 7.0 and 6.8 (IH, 2 x t) , 5.0-4.2 (3H, m) , 3.8 (3H, m) , 3.4-2.5 (6H, m) , 1.9 (3H, m) , 1.5 (6H, m) , 1.1 (6H, m) Ex.43 (d b -DMSO) δ 13.0 (2H, br s) , 9.8 (IH, d) , 8.0 (IH, d) , 7.9- 6.8 (17H, m) , 4.5-4.2 (3H, m) , 3.1 (2H, m) , 2.8 (2H, m) , .2.6 (IH, m) , 2.3 (IH, m) , 1.8 (3H, m) , 1.5 (6H, m) , 1.1 (6H, m) Ex.44 (d 6 -DMSO) δ 9.8 (IH, m) , 8.4-8.0 (3H, m) , 7.1 (14H, m) , 4.5 (3H, m) , 3.0 (4H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.8 (3H, m) , 1.5 (6H, m) , 1.1 (6H, s) Ex.45 (d 6 -DMSO) δ 13.7 (IH, br s) , 8.5 (2H, ) , 8.2-7.9 (2H, m) , 7.6-6.4 (16H, m) , 4.5 (IH, ε) , 4.4 (IH, ) , 4.2 (IH, s) , 3.5-3.2 (2H, m) , 2.9 (2H, m) , 2.6 (IH, m) , 2.3 (IH, m) , 1.7 (3H, m) , 1.5 (6H, m) , 1.0 (6H, m) Ex.46 (d 6 -DMSO) δ 12.9 (IH, br s) , 9.7 (IH, ε) , 8.2 (IH, ε) , 7.6- 7.0 (17H, m) , 6.8 (IH, t) , 4.5 (3H, m) , 3.2 (IH, ) , 3.0 (2H, m) , 2.9 (IH, m) , 2.4 (IH, m) , 1.8 (3H, m) , 1.5 (6H, m) , 1.3 (6H, m) Ex.47 (d 6 -DMSO) δ 9.8 (IH, s) , 8.0 (IH, s) , 7.9 (IH, d) , 7.8-6.9 (17H, m) , 4.5 (2H, m) , 4.2 (IH, ) , 3.4-3.1 (2H, ) , 2.8 (IH, d) , 2.7-2.3 (3H, m) , 1.9 (3H, m) , 1.5 (6H, ) , 1.2 (6H, m) Ex.48 (d 6 -DMSO) δ 9.8 (IH, ε) , 8.4 (2H, s) , 8.1 (IH, s) , 8.0 (IH, d), 7.1 (14H, m) , 4.5 (2H, s) , 4.2 (IH, ε) , 3.9 (6H, ε) , 3.1 (2H, ) , 2.8 (2H, m) , 2.6 (IH, m) , 2.2 (IH, m) , 1.7 (3H, ε) , 1.5 (6H, m) , 1.1 (6H, ε) Ex.49 (CDC1,) δ 9.2 and 9.1 (IH, 2 x s) , 8.4-8.0 (4H, m) , 7.1 (14H, m) , 4.8 (IH, m) , 4.5 (2H, m) , 3.6-2.62.6 (4H, m) , 1.7 (3H, m) , 1.5 (6H, m) , 1.1 (6H, ) , 0.7 (6H, m) Ex.50 (d b -DMSO) δ 10.5 (IH, s) , 9.9 (IH, ε) , 8.5 (2H, ε) , 8.1 (IH, s), 7.9 (IH, d) , 7.7 (IH, br t) , 7.4-6.7 (18H, m) , 4.6 (IH, m) , 4.4 (IH, s), 4.1 (IH, s), 3.3 (2H, m) , 3.0 (2H, m) , 2.9-2.7 (2H, m) , 2.6 (2H, m) Ex.51 (d-DMSO) δ 13.2 (2H, br s) , 10.8 (IH, s) , 10.0 (IH, ε) , 8.3 (2H, d), 8.1 (IH, d) , 7.6 (IH, br t) , 7.5-6.8 (19H, m) , 4.5 (IH, m) , 4.45 (IH, d) , 4.37 (IH, d) , 3.3-2.8 (6H, m) , 2.7 (2H, m) Ex.52 (d 6 -DMSO) δ 9.9 (IH, ε) , 8.5 (2H, s) , 8.2 (IH, br s) , 8.1 (IH, ε), 7.4-6.7 (17H, ) , 4.5 (2H, m) , 4.2 (3H, m) , 3.0 (4H, m) Ex.53 (d 6 -DMSO) δ 13.2 (2H, br ε) , 10.0 (IH, br ε) , 8.5 (2H, s) , 8.2 (IH, br s) , 8.1 (IH, s) , 7.7 (IH, br s) , 7.5-6.8 (18H, m) , 4.5 ■ (3H, m) , 3.0 (6H, m) , 2.5 (2H, m) Ex.54 (d b -DMSO) δ 13.2 (2H, br s) , 10.0 (IH, 2xε) , 8.3 (2H, s) , 8.1 (IH, br s), 8.0 (IH, d) , 7.5-6.8 (19H, m) , 4.5 (3H, m) , 3.0 (6H, m), 2.0 (2H, m) , 1.5 (2H, t) Ex.55 (d 6 -DMSO) δ 13.2 (IH, br s) , 9.7 (IH, s) , 8.2 (IH, s) , 8.0 (IH, br s), 7.8 (IH, d) , 7.6 (IH, d) , 7.4-6.9 (13H, m) , 4.5 (3H, m) , 3.0 (4H, m) , 2.4 (2H, m) , 1.8 (3H, d) , 1.5 (6H, m) , 1.2 (6H, m) Ex.56 (d 6 -DMSO) δ 13.0 (IH, br s) , 9.7 (IH, s) , 8.2 (IH, s) , 7.6 (2H, m) , 7.4-6.8 (14H, ) , 4.5 (3H, m) , 3.0 (4H, m) , 2.4 (2H, m) , 1.8 (3H, s), 1.5 (6H, q) , 1.2 (6H, s) Ex.57 (d c -DMSO) δ 13.0 (IH, br s) , 9.7 (IH, ε) , 8.3 (IH, ε) , 8.2 (IH, br s), 8.0-6.9 (22H, m) , 4.7-4.2 (5H, m) , 3.0 (4H, m) Ex.58 (d 6 -DMSO) δ 13.0 (IH, br s) , 9.8 (IH, s) , 8.2 (2H, br s) , 8.0-6.9 (22H, m) , 4.7-4.2 (5H, m) , 3.0 (4H, m) Ex.59 (d 6 -DMSO) δ 12.8 (IH, br s) , 9.8 (IH, s) , 8.3 (IH, br s) , 8.2 (IH, ε), 8.0-6.9 (24H, m) , 4.7-4.2 (5H, m) , 3.0 (4H, m) Ex.60 (d b -DMSO) δ 12.8 (IH, br s) , 9.8 (IH, s) , 8.4 (IH, br s) , 8.3 (IH, ε), 8.0-6.9 (24H, m) , 4.7-4.2 (5H, m) , 3.0 (4H, m) Ex.61 (d e -DMSO) δ 8.3 (2H, m) , 8.0 (2H, s) , 7.8 (IH, d) , 7.4 (IH, t), 7.3-6.9 (13H, m) , 4.5 (IH, s) , 4.3 (3H, m) , 4.1 (IH, s) , 3.2- 2.4 (6H, m) , 1.8 (3H, m) , 1.5 (6H, m) , 1.2 (6H, m) Ex.62 (d 6 -DMSO) δ 8.3 (2H, m) , 8.0 (2H, s) , 7.4-7.0 (12H, t) , 6.9 (3H, m) , 4.4 (2H, 2xs) , 4.3 (IH, m) , 4.2 (2H, m) , 3.2-2.4 (6H, ) , 1.8 (3H, m) , 1.6 (6H, m) , 1.2 (6H, m) Ex.63 (d 6 -DMSO) δ 13.2 (2H, br s) , 9.8 (IH, ε) , 8.4 (2H, s) , 8.3 (IH, m) , 8.1 (2H, m) , 7.7 (3H, m) , 7.5 (IH, s) , 7.3 (9H, m) , 7.2 (2H, m) , 7.1 (2H, m) , 6.9 (3H, s) , 4.6 (IH, s) , 4.55 (IH, m) , 4.4 (IH, m) , 4.3 (IH, ε), 4.1 (IH, m) , 3.2 (2H, m) , 2.9 (IH, m) , 2.8 (IH, m) Ex.64 (d 6 -DMΞO) δ 13.2 (2H, br s) , 9.9 (IH, s) , 8.4 (2H, s) , 8.3 (IH, m), 8.1 (2H, m) , 7.7 (3H, m) , 7.5 (IH, s) , 7.3 (9H, m) , 7.2 (2H, m) , 7.1 (2H, m) , 6.9 (3H, s) , 4.6 (IH, s) , 4.55 (IH, m) , 4.4 (IH, m) , 4.3 (IH, s) , 4.1 (IH, m) , 3.2 (2H, ) , 2.9 (IH, m) , 2.8 (IH, m) The compounds of the examples were tested for binding at the CCKg receptor in mouse cortical membranes by means of a radioligand binding assay. The procedure was as follows: The whole brains from male mice (CD1 22-25g; Charles River) were removed and placed in ice-cold buffer (pH7.2 @ 21 ± 3°C) of the following composition (mM) ; 10 HEPES, 130 NaCI, 4.7 KC1, 5 MgCl 2 , 1 EDTA and containing 0.25g.l _1 bacitracin. The cortex was disεected, weighed and homogeniεed in 40ml ice-cold buffer using a Teflon-in-glass homogeniser. The homogenate was centrifuged at 39,800g for 20 min at 4°C, the supernatant discarded and the pellet resuεpended by homogeniεation in freεh buffer. The homogenate waε recentrifuged (39,800g; 20 min & 4°C) and the final pellet waε resuspended in HEPES buffer to give a tissue concentration of 2mg.ml "1 (original wet weight) . The membranes (400ml) were incubated for 150 min at 21 ± 3°C in a final volume of 0.5ml with HEPES buffer containing [ ι:: -T]-CCK8S (0.05ml; 200pM NEN 2200Ci.mmol ": ) and competing corrpound. Total and non-specific binding of [ 1 I]-CCK8S were defined using 0.05ml of buffer and 0.05ml of lOmM L-365,260, respectively. The assay was terminated by rapid filtration through pre-εoaked Whatman GF/B filters using a Brandell Cell harvester. The filters were washed (3 x 3ml) with ice-cold 50mM Tris-HCl (pH7.4 @ 4°C) and bound radioactivity determined by counting (1 min.) in a gamma-counter. The results obtained from the CCK E asεays are εet out in Table 1. TABLE 1 Example CCKg pK d Exaπple CCKg pKi 1 8.8 34 7.4 2 7.8 35 6.8 3 8.0 36 7.2 4 7.8 37 7.0 5 7.6 38 7.3 6 8.6 39 6.3 7 7.3 40 7.8 8 7.9 41 6.8 9 7.3 42 7.5 10 8.0 43 7.0 11 6.6 44 6.5 12 7.0 45* 6.7 13 6.3 46* 6.8 14 6.5 47* 6.9 15 6.7 48* 5.0 16 8.5 49 7.2 17 8.2 50 7.8 18 8.5 51 7.1 19 8.0 52 7.3 20 8.0 53 8.4 21 7.3 54 7.5 22* 5.4 55 8.2 23* 5.4 56 7.6 24 8.8 57 7.2 27 7.8 58 6.3 28 8.4 59 7.2 29 7.4 60 6.4 30 7.7 61 7.5 31 6.7 62 7.4 32 8.0 63 8.6 33 6.5 64 8.3 * - comparative examples The coπpounds of the examples were also tested for gastrin antagonist activity in an immature rat stomach asεay. The procedure was as follows: The oeεophagus of immature rats (33-50 g, ca. 21 days old) was ligated at the level of the cardiac sphincter and the duodenal sphincter was cannulated. The stomach was excised and flushed with ca. 1 ml of unbuffered physiological saline solution. The fundus was punctured and cannulated. A further 4-5 ml of unbuffered solution was flushed through the stomach to ensure the preparation was not leaking. The stomach was lowered into a jacketed organ bath containing 40 ml of buffered solution containing 3xlO "8 M 5-methylfurmethide, maintained at 37° and gassed vigorously with 95% 0 2 / 5% C0 2 . The stomach was continuously perfused at a rate of 1 ml min "1 with unbuffered solution gassed with 100% 0 2 with the perfusate passing over an internally referenced pH-electrode fixed 12 cm above the stomach. After 120 min of stabilisation the drugs were added directly to the serosal solution in the organ bath and after a further 60 min cumulative pentagastrin dose-response curves were started. Changes in acid secretion were monitored and the curves analysed according to Black et.al., Br. J. Pharmacol., 1985, .86, 581. The results obtained from the gastrin assays are set out in Table 2. TABLE 2 ample Gastrin pKg Exaπple Gastrin pKg 1 8.3 28 8.4 2 7.3 29 7.0 3 6.9 30 7.3 4 7.8 31 6.6 5 6.5 32 7.6 6 7.9 34 6.5 7 6.6 35 6.0 8 8.1 36 7.5 9 7.7 37 7.2 10 8.3 38 7.1 11 7.0 39 6.4 12 7.2 40 5.7 13 6.5 41 5.7 14 6.9 42 5.8 15 5.8 43 6.1 16 8.6 44 6.9 17 8.0 45* - 18 8.4 46* 5.7 19 7.6 47* - 20 8.2 48* - 21 7.2 49 8.0 23* 6.6 53 7.1 24 8.9 61 7.4 25 7.2 62 7.2 26 6.8 63 7.4 27 8.1 64 6.5 * - comparative examples The corrpounds of the examples were also tested in a CCK A binding assay as follows: The pancreatata were removed from male guinea-pigs (200-300g; Dunkin Hartley) and placed in ice-cold HEPES buffer (pH 7.2 @ 21 ± 3 * ) . The pancreatata were homogenised in 40 ml ice-cold HEPES buffer using a polytron (Brinkmann, PT10, setting 10) 4 x 1 second. The homogenate was centrifuged at 39,800g for 15 min at 4 * . The supernatant was discarded and the pellet re-suspended using a Teflon-in-glass homogeniser in 20 volumes of fresh buffer and re¬ centrifuged as above. The final pellet was re-suspended using a Teflon-in-glass ' homogeniser to a tissue concentration of 1 mg. l "1 (original wet weight) , and filtered through 500 μm pore-size Nytex mesh. The membranes (400μl; containing 0.375 μM PD134,308) are incubated for 150 minuteε at 21 ± 3' in a final volume of 0.5ml with HEPES buffer containing [ 1:? I] -CCK b (S) (50μl; 200pM) and competing coπpound. Total and non-specific binding of [ 1:' -'I] -CCK 8 (S) are defined using 50μl of buffer and 50μl of lOOnM L-364,718 respectively. The assay is terminated by rapid filtration through pre-soaked Whatman GF/B filters using a Brandell Cell Harvester. The filters were washed (3 x 3ml) with ice-cold 50mM Tris HCI (pH 7.4 at 4" ) and bound radioactivity is determined by counting (1 min) in a gamma counter. The results are set out in Table 3. TABLE 3 Exaπple CCK A pK j Exarrple CCK A pKj 1 5.7 33 5.9 2 6.3 34 6.6 3 5.6 35 5.9 4 5.9 36 5.7 5 6.0 37 5.7 6 5.7 38 6.5 7 6.3 39 5.6 8 6.0 40 6.0 9 6.4 41 6.0 10 6.1 42 5.7 11 7.3 43 5.9 12 5.8 45* 5.9 13 5.7 49 5.6 14 6.1 50 6.4 15 7.0 51 5.8 16 6.0 52 6.8 17 6.1 53 5.5 18 6.5 54 5.8 19 6.4 55 6.1 20 5.7 56 6.0 21 5.9 61 5.6 22* 5.9 62 5.6 30 5.4 63 5.8 31 5.9 64 5.8 32 6.3 * - comparative examples

Claims: CLAIMS 1. A coπpound of the formula wherein R 1 and R 2 are independently H, methyl, halo, carboxy, esterified carboxy, amidated carboxy, carboxymethyl, esterified carboxymethyl or amidated carboxymethyl, R 3 and R 4 (or each R 3 and R 4 group, when m or n is 2 or more) are independently selected from halo, amino, nitro, cyano, sulphamoyl, sulphonyl, trifluoromethyl, C_ to C 3 alkyl, C-* to C 3 alkoxy, hydroxy, C : to C 3 hydroxyalkyl, C : to C 3 alkylcarboxyamino, carboxy, esterified carboxy and amidated carboxy R 5 and R 6 are independently selected from H and the groups recited above for R 3 m is from 0 to 4, provided that m is not more than 2 unless R 3 is exclusively halo, n is from 0 to 4, provided that n is not more than 2 unless R 4 is exclusively halo, R 7, R 9 and R 1 are independently H or C : to C 3 alkyl, R 8 is H or C α to C,r, hydrocarbyl, in which one or more hydrogen atoms of the hydrocarbyl group may be replaced by a halogen atom, and up to two of the carbon atoms may be replaced by a nitrogen, oxygen or sulphur atom, provided that R £ does not contain a -0-0- group, U is aryl, substituted aryl, heterocyclic, substituted heterocyclic or cycloalkyl, and Z is a group of the formula (wherein R is H or C : to C 5 alkyl, X is -C0 2 H or tetrazolyl, Y is H, -C0 2 H, tetrazolyl, -CH 2 OH, -CC e or -CONH 2 , and a is from 0 to 2) or a pharmaceutically acceptable salt thereof. 2. A compound according to claim 1, wherein R 8 is C b to C 8 straight or branched chain alkyl or cycloalkyl, or R 10 -(CH 2 ) P -, wherein R 10 is selected from phenyl, 1-naphthyl, 2-naphthyl, indolyl, norbomyl, 1-adamantyl, 2-adamantyl, cyclohexyl or cycloheptyl, and p is from 0 to 3. 3. A coπpound according to claim 1 or claim 2, wherein R 1 and R 2 are both H. 4. A coπpound according to any preceding claim, wherein R 5 and R 6 are both H. 5. A coπpound according to any preceding claim, wherein m and n are both 0. 6. A corrpound which is degraded in vivo, to yield a corrpound according to any preceding claim. 7. A compound of the formula wherein R : , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , m, n and U are aε defined in claim 1, and Z is a group of the formula 'a (wherein R is as defined in claim 1; X iε eεterified carboxy or amidated carboxy, and Y iε H, -C0 2 H, esterified carboxy, amidated carboxy, tetrazolyl or -CH 2 OH, or X is -C0 2 H or tetrazolyl, and Y is esterified carboxy or amidated carboxy; and a iε from 0 to 2) or a pharmaceutically acceptable salt thereof. 8. A pharmaceutical corrposition comprising a corrpound according to any preceding claim, together with a pharmaceutically acceptable diluent or carrier. 9. A method of making a corrpound according to any of claims 1 to 5, said method including the step of reacting a corrpound of the formula with a suitably protected coπpound of formula 10. A method of making a corrpound according to any of claims 1 to 5, said method including the step of reacting a corrpound of the formula with a suitably protected corrpound of formula X H-N-( CH 2 ) a — Or H-H ( CH 2 ) i Y 11. A method of making a corrpound according to any of claims 1 to 5, said method including the step of reacting a corrpound of the formula with a suitably protected corrpound of formula HNR 7 R 8 12. A method of making a composition according to claim 6, said method comprising admixing a coπpound according to any of claims 1 to 5 with a pharmaceutically acceptable diluent or carrier.

Inventors: HUDSON MARTIN LYN, KALINDJIAN SARKIS BARRET, STEEL KATHERINE ISOBEL MARY, TOZER MATTHEW JOHN, HUDSON, MARTIN, LYN, KALINDJIAN, SARKIS, BARRET, STEEL, KATHERINE, ISOBEL, MARY, TOZER, MATTHEW, JOHN
Applicants: BLACK JAMES FOUNDATION, HUDSON MARTIN LYN, KALINDJIAN SARKIS BARRET, STEEL KATHERINE ISOBEL MARY, TOZER MATTHEW JOHN, HUDSON, MARTIN, LYN, JAMES BLACK FOUNDATION LIMITED, KALINDJIAN, SARKIS, BARRET, STEEL, KATHERINE, ISOBEL, MARY, TOZER, MATTHEW, JOHN
Application year: 1994
Main classification: C07C237
IPC codes: A61K31, A61P1, A61P35, C07C237, C07D209, C07D257, C07D333