NEW CRYPTOPHYCINS

Abstract: The present invention provides novel cryptophycin compounds having structure (I). The present invention also provides methods for producing cryptophycins from the Nostoc sp. of blue-green algae (cyanobacteria). Pharmaceutical compositions comprising novel cryptophycins are also provided by the present invention, as are methods for using cryptophycins to inhibit the proliferation of hyperproliferative cells. The present invention further provides methods for using cryptophycins to inhibit the proliferation of hyperproliferative cells with drug resistant phenotypes, and to treat pathological conditions, such as neoplasia.

Description: NEW CRYPTOPHYCINS Background of the Invention Neoplastic diseases, characterized by the proliferation of cells not subject to the norm control of cell growth, are a major cause of death in humans. Clinical experience in cance chemotherapy has demonstrated that new and more effective drugs are desirable to treat thes diseases. Such clinical experience has also demonstrated that drugs which disrupt th microtubule system of the cytoskeleton can be effective in inhibiting the proliferation o neoplastic cells. The microtubule system of eucaryotic cells is a major component of the cytoskeleton an is in a dynamic state of assembly and disassembly; that is, heterodimers of tubulin ar polymerized and form microtubules. These microtubules play a key role in the regulation of cel architecture, metabolism, and division and their dynamic character is essential to their norma function in the cell. For example, with respect to cell division, microtubules are created, tha is, polymerized from tubulin, to form the mitotic spindle. When the mitotic spindle's use ha been fulfilled, the microtubules contained in it are subsequently depolymerizd. Disrupting eithe the polymerization or depolymerization of microtubules in the cell can inhibit mitosis, an thereby cell proliferation. An agent which could prevent a cell from proliferating by inhibiting a cells' microtubul dynamic state would be useful in treating cancer, a disease characterized by cells proliferatin at abnormally high rates. Indeed, such agents comprise some of the most effective cance chemotherapeutic agents in clinical use today. These anti-mitotic agents or poisons are classified into three groups based on thei molecular mechanism of action. The first group, including colchicine and colcemid, inhibit th formation of microtubules by sequestering tubulin. The second group, including vinblastine an vincristine, induce the formation of paracrystalline aggregates of tubulin. These two agent' action preferentially inhibits the proliferation of hvperproliferating cells by disrupting mitotic spindle microtubules. The third group, including taxol, promotes the polymerization of tubulin, thereby disrupting the dyanmic state of microtubule polymerization and depolymerization. However, an agent's having activity as an antimitotic poison does not lead to th concluaion that this agent would evince such activity in a tumor cell nor in a tumor cell with drug-resistant phenotype. For example, vi ca alkaloids such as vinblastine and vincristine ar effective against some neoplastic cells and tumors, yet they lack activity against some drug resistant tumors and cells. One basis for a neoplastic cell displaying drug resistance (DR) o multiple-drug resistance (MDR) is through the over-expression of P-glycoprotein. Compoun which are poor substrates for transport of P-glycoprotein should be useful in circumventing su a MDR phenotype. Accordingly, the exhibition of the DR or MDR phenotype by many tumor cells and t clinically proven mode of action of anti-microtubule agents against neoplastic cells necessitat the development of anti-microtubule agents cytotoxic to non-drug resistant neoplastic cells as we as cytotoxic to neoplastic cells with a drug resistant phenotype. Background Art Selected cryptophycin compounds, dioxa-diazacyclohexadecenetetrones isolated synthesized from isolates from the blue-green algae (cyanobacteria) of the genus Nostoc, we characterized as antifungal agents with activity toward filamentous fungi, specifically t Aspergillus, Penicillium and Phoma species thereof; however, their mechanism of action w unknown. Five cryptophycin compounds, herein designated Cryptophycins 1, 3, 5, 13 and 1 were disclosed in U.S. Patent Nos. 4,946,835, 4,845,085, 4,845,086, and 4,868,208, suc compounds either having been isolated from a strain of Nostoc sp. designated MB 5357 or havin been synthesized from such an isolated compound. Summary of the Invention The present invention provides novel cryptophycin compounds having the followi structure: Wherein R, is H, OH, a halogen, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alk group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; o R, and R, may be taken together to form an epoxide ring, an aziridene ring, a sulfide gring o a second bond between C 10 and C„; or R, and R 4 may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R„ is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; R 5 is H or an OH group; R 6 is H; or Rj and R^ may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxybenzyl, methoxybenzyl, halohydroxy benzyl, dihalohydroxybenzyl halomethoxybenzyl, or dihalomethoxybenzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterifie lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R 4 and R 8 may be taken together to form a didepsipeptide group consisting of a lower β-amin acid bonded to a lower α-hydroxy alkanoic acid; or R j and R 8 may be taken together to form a didepsipeptide group consisting of a lower β-amin acid bonded to a lower α-hydroxy alkanoic acid; with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a keto group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a keto group, NH 2 , SH; when R, is OH, R 2 is OH, R 3 is methyl, Rj and ^ are taken together to form a secon bond between C 5 and C 6 , R 4 and R 8 are taken together to form the didepsipeptide grou with the structure X: wherein O, of X corresponds to R 4 , N 8 of X corresponds to R 8 , R, is methyl, an R 10 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; when R, and R 2 are taken together to form an epoxide ring, R 3 is methyl, R 5 and R « ar taken together to form a second bond between C 5 and C 6 , R, and R 8 are taken togethe to form a didepsipeptide with the structure X, R, is methyl, and R I0 is isobutyl, R 7 is no 3 -chloro-4-methoxy benzyl; when R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 i methyl, > and R^ are taken together to form a second bond between C 5 and C 6 , R 4 and R 8 are taken together to form a didepsipeptide with the structure X, R, is methyl, and R ] is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; and when R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 and 1^ are taken together to form a second bond between C 5 and C 6 , R 4 is bonded to the carboxy terminus of leucic acid, and R 8 is bonded to the nitrogen terminus of either 3-amino-2- methylpropionic acid or 3-amino-2-methylpropionic acid methyl ester, R 7 is not 3-chloro- 4-methoxybenzyl . Further provided by the present invention are methods for producing both novel and previously -disclosed cryptophycin compounds from the Nostoc sp. of blue-green algae (cyanobacteria). Pharmaceutical compositions comprising the new cryptophycin compounds are also provided by the present invention, as are methods for using both novel and previously- disclosed cryptophycin compounds to inhibit the proliferation of normal and hyperproliferativ mammalian cells. The present invention also provides methods for using both novel an previously disclosed cryptophycin compounds to inhibit the proliferation of hyperproliferativ mammalian cells with drug-resistant phenotypes, including those with multiple drug-resista phenotypes. Furthermore, methods of using both novel and previously-disclosed cryptophyci compounds to treat pathological conditions, such as neoplasia, are provided by the prese invention. Brief Description of the Figures Figure 1 provides a general structure of selected cryptophycin compounds of the presen invention and a numbering system for the hydroxy acid units A and D and two amino acid unit B and C in the selected embodiments. Figure 2 graphically presents the effects of cryptophycin compounds and vinblastine o Jurkat cell proliferation and cell cycle progression. Jurkat cells were incubated with the indicate concentrations of cryptophycin compounds (A) or vinblastine (B) for 24 hours. For each sample the number of viable cells (■) and the mitotic index (□) were determined as described in th Experimental section. Values represent the means ± standard deviation (sd) for triplicat samples in one of three similar experiments. Figure 3 graphically presents "the reversibility of the effects of vinblastine, cryptophycin and taxol on cell growth. SKON3 cells were treated with 0.1 nM vinblastine (π), 0.1 n cryptophycins (■) or 1 nM taxol (B) at time = 0. These concentrations inhibited cell growt by 50% for each compound. After 24 hours the cells were washed and incubated in drug-fre medium for the time indicated. The cell density v/as determined by sulforhodamine B (SRB staining as described in the Experimental Section, and is expressed as the mean + sd absorbanc at 560 nm for triplicate samples in one of three experiments. Figure 4 provides Isobolograms for combinational effects of vinblastine and cryptophycin on cell proliferation. SKON3 cells were treated with vinblastine (0-600 pM) and/o cryptophycins (1-100 pM) for 48 hours. Cell numbers were then determined by SRB stainin as described in the Experimental Section, and the IC 50 s (■) and the line of additivity ( — ) fo combinations of vinblastine and cryptophycin compounds. Values represent the means for tw experiments each containing triplicate samples. Detailed Description of the Invention The present invention provides novel cryptophycin compounds having the followi structure: Wherein R, is H, OH, a halogen, O of a ketone group, NH,, SH, a lower alkoxyl group or a lower alk group; R, is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; R, and R 2 may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or second bond between C,„ and C n ; or R, and R 4 may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R 4 is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; R 5 is H or an OH group; -R s is H; or - 5 and - j may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxybenzyl, methoxybenzyl, halohydroxybenzyl, dihalohydroxybenzyl halomethoxybenzyl, or dihalomethoxybenzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterifie lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R, and R 8 may be taken together to form a didepsipeptide group consisting of a lower β-amin acid bonded to a lower α-hydroxy alkanoic acid; and R, and R 8 may be taken together to form a didepsipeptide group consisting of a lower β-amin acid bonded to a lower α-hydroxy alkanoic acid; and with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R 2 is OH, O of a keto group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a keto group, NH 2 , SH; when R, is OH, R 2 is OH, R 3 is methyl, R; and B^ are taken together to form a secon bond between C 5 and C 6 , R 4 and R 8 are taken together to form the didepsipeptide grou with the structure X: wherein O, of X corresponds to R 4 , N 8 of X corresponds to R 8 , R, is methyl, an R 10 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; when R, and R 2 are taken together to form an epoxide ring, R 3 is methyl, R 5 and R^ ar taken together to form a second bond between C 5 and C 6 , R 4 and R 8 are taken togethe to form a didepsipeptide with the structure X, - e, is methyl, and R 10 is isobutyl, R 7 is no 3-chloro-4-methoxybenzyl ; when R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 i methyl, -R, and -R e are taken together to form a second bond between C 5 and C 6 , R 4 an R 8 are taken together to form a didepsipeptide with the structure X, R, is methyl, and R, is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; and when R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R; and R^ ar taken together to form a second bond between C 5 and C 6 , R 4 is bonded to the carbox terminus of leucic acid, and R 8 is bonded to the nitrogen terminus of either 3-amino-2 methylpropionic acid or 3-amino-2-methylpropionic acid methyl ester, R 7 is not 3-chloro 4-methoxybenzyl. The invention further provides cryptophycin compounds wherein at least one of th groups attached to C 2 , C 8 , C 9 , C I0 , and C„ has R stereochemistry. In a further embodiment o the invention, at least one of the groups attached to C„ C 8 , C 9 , C 10 , and C„ has S stereochemistry The invention further provides cryptophycin compounds in accordance with the abo structure where the structure of the didepsipeptide that is formed when R 4 or R j is taken togeth with R 8 is the following structure X: wherein O, of X corresponds to R 4 or -R^, N 8 of X corresponds to R 8 , R, is H or a lowe alkyl group, and R I0 is H or a lower alkyl group. As used herein, "lower β-amino acid" means any β-amino acid having three to eigh carbons and includes linear and non-linear hydrocarbon chains; for example, 3-amino-2 methylpropionic acid. As used herein, "esterified lower β-amino acid" means any β-amino aci having three to five carbons where the hydrogen of the carboxylic acid group is substituted wit a methyl group; for example, 3-amino-2-methylpropionic acid methyl ester. As used herein "lower alkanoyloxy group" means an alkanoyloxy group of one to seven carbons and include linear and non-linear hydrocarbon chains. As used herein, "lower α-hydroxyalkanoyloxy group means an α-hydroxyalkanoyloxy group of two to seven carbons and includes linear and non linear hydrocarbon chains; for example, 2-hydroxy-4-methylvaleric acid. As used herein, "lower alkoxyl group" means any alkyl group of one to five carbon bonded to an oxygen atom. As used herein, "lower alkyl group" means an alkyl group of on to five carbons and includes linear and non-linear hydrocarbon chains. As used herein, "epoxide ring" means a three-membered ring whose backbone consist of two carbons and an oxygen atom. As used herein, "aziridine ring" means a three-membere ring whose backbone consists of two carbons and a nitrogen atom. As used herein, "sulfid ring" means a three-membered ring whose backbone consists of two carbons and a sulfur atom. As used herein, "halogen" refers to those members of the group on the periodic tabl historically known as the halogens. Methods of halogenation include, but are not limited to, th addtion of hydrogen halides, substitution at high temperature, phohalogenation, etc. , and suc methods are known to those of ordinary skill in the art. 1 2 An example of a novel cryptophycin compound of the present invention is when R, a R 2 are taken together to form an epoxide group, R 3 is methyl, -R, and -R^ are taken together form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 4-methoxybenzy and R 4 and R 8 are taken together to form the didepsipeptide with the structure X where R, methyl and R 10 is isobutyl. The structure of this cryptophycin compound, Cryptophycin 2, is t following: CRYPTOPHYCIN 2 A further example of a novel cryptophycin compound of the present invention is whe R ( and R 2 are taken together to form a second bond between the C, 0 and C„ carbons such th there is a double bond, R 3 is methyl, R 5 and R « are taken together to form a second bond betwee C 5 and C 6 such that there is a double bond, R 7 is 4-methoxy benzyl, and R 4 and R 8 are take together to form the didepsipeptide with the structure X where R, is methyl and R 10 is isobutyl The structure of this cryptophycin compound, Cryptophycin 4, is the following: CRYPTOPHYCIN 4 A further example of a novel cryptophycin compound of the present invention is whe R, and R, are taken together to form a tetrahydrofuran ring, R 2 is an OH group, R 3 is methyl R s and R « are taken together to form a second bond between C 5 and C 6 such that there is a doubl bond, R 7 is 3 -chloro-4-methoxy benzyl, and R 8 is a (2-carbomethoxypropyl)amino group. Th structure of this cryptophycin compound, Cryptophycin 6, is the following: CRYPTOPHYCIN 6 A further example of a novel cryptophycin compound of the present invention is whe R, and R 4 are taken together to form a tetrahydrofuran ring, R 2 and R 8 are OH groups, R 3 i methyl, R j and -R s are taken together to form a second bond between C 5 and C 6 such that ther is a double bond, and R 7 is 3-chloro-4-methoxybenzyl. The structure of this cryptophyci compound, Cryptophycin 7, is the following- CRYPTOPHYCIN 7 A further example of a novel cryptophycin compound of the present invention is whe R, is a chloro group, R 2 is an OH group, R 3 is methyl, Rj and 1^ are taken together to form second bond between C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4 methoxybenzyl, and R 4 and R 8 are taken together to form the didepsipeptide with the structur X where -R, is methyl and R, 0 is isobutyl. The structure of this cryptophycin compound Cryptophycin 8, is the following: CRYPTOPHYCIN 8 A further example of a novel cryptophycin compound of the present invention is whe R, is a methoxy group, R 2 is an OH group, R 3 is methyl, ^ and -R^ are taken together to for a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3-chloro- methoxybenzyl, and R, and R 8 are taken together to form the didepsipeptide with the strucmr X where R, is methyl and R 10 is isobutyl. The strucmre of this cryptophycin compound Cryptophycin 9, is the following: CRYPTOPHYCIN 9 A further example of a novel cryptophycin compound of the present invention is whe R, is a methoxy group, R 2 and R,are OH groups, R 3 is methyl, Rj and Rg are taken together t form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4 methoxybenzyl, and R 8 is a (2-carboxypropyl)amino group. The strucmre of this cryptophyci compound, Cryptophycin 10, is the following: CRYPTOPHYCIN 10 A further example of a novel cryptophycin compound of the present invention is whe R, and R 4 are taken together to form, a tetrahydrofuran ring, R, is an OH group, R 3 is methyl, R s and R^ are taken together to form a second bond between C 5 and C 6 such that there is a doubl bond, R 7 is 3-chloro-4-methoxybenzyl, and R 8 is a (2-carboxypropyl)amino group. The strucmr of this cryptophycin compound, Cryptophycin 12, is the following: CRYPTOPHYCIN 12 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between the C,„ and C„ carbons such tha there is a double bond, R 3 is methyl, R 4 is an OH group, Rj and R^ are taken together to for a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4 methoxybenzyl, and R 8 is a (2-carboxypropyl)amino group. The strucmre of this cryptophyci compound, Cryptophycin 14, is the following: CRYPTOPHYCIN 14 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form an epoxide group, R 3 is methyl, -R; and Rg are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3- chloro-4-hydroxybenzyl, and R, and R 8 are taken together to form the didepsipeptide with the strucmre X where R, is methyl and R 10 is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 16, is the following: CRYPTOPHYCIN-16 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between C 10 and C„ carbons such that ther is a double bond, R 3 is methyl, Rj and -Rg are taken together to form a second bond between and C 6 such that there is a double bond, R 7 is 3-chloro-4-hydroxybenzyl, and R 4 and R 8 are take together to form the didepsipeptide with the strucmre X where R, is methyl and R 10 is isobutyl The strucmre of this cryptophycin compound, Cryptophycin 17, is the following: CRYPTOPHYCIN-17 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between C, 0 and C„ carbons such that ther is a double bond, R 3 is methyl, R 5 and R^ are taken together to form a second bond between C and C 6 such that there is a double bond, R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are take together to form the didepsipeptide with the strucmre X where R, is methyl and R, 0 is sec-butyl The structure of this cryptophycin compound, Cryptophycin 18, is the following: CRYPTOPHYCIN-18 A further example of a novel cryptophycin compound of the present invention is wh R, and R, are taken together to form a second bond between C 10 and C„ carbons such that the is a double bond, R 3 is methyl, Rj and I^ are taken together to form a second bond between and C 6 such that there is a double bond, R, is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are take together to form the didepsipeptide with the strucmre X where R, is methyl and R 10 is isopropy The strucmre of this cryptophycin compound, Cryptophycin 19, is the following: CRYPTOPHYCIN-19 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 and R « are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3 chloro-4-methoxybenzyl, and R, and R 8 are taken together to form the didepsipeptide with th strucmre X where R, is hydrogen and R, 0 is isobutyl. The strucmre of this cryptophyci compound, Cryptophycin 21, is the following: CRYPTOPHYCIN-21 A further example of a novel cryptophycin compound of the present invention is whe R, and R, are taken together to form an epoxide group, R 3 is methyl, R 5 and R^ are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3,5 dichloro-4-hydroxybenzyl, and R 4 and R 8 are taken together to form the didepsipeptide with th strucmre X where -R, is methyl and R ]0 is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 23, is the following: CRYPT0PHYCIN-23 A further example of a novel cryptophycin compound of the present invention is whe Ri and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 and 1^ are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 4 methoxybenzyl, and R 4 and R 8 are taken together to form the didepsipeptide with the strucmr X where R, is hydrogen and R 10 is isobutyl. The strucmre of this cryptophycin compound Cryptophycin 24, is the following: CRYPTOPHYCIN-24 A further example of a novel cryptophycin compound of the present invention is whe R t and R 2 are taken together to form a second bond between C 10 and C„ carbons such that ther is a double bond, R 3 is methyl, R 4 is hydroxy, R^ is hydrogen, R 7 is 3-chloro-4-methoxybenzyl and -R 5 and R 8 are taken together to form the didepsipeptide with the strucmre X where R, i methyl and R 10 is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 26, i the following: CRYPTOPHYCIN-26 A further example of a novel cryptophycin compound of the present invention is wh R, and R 2 are taken together to form a second bond between the C, 0 and C„ carbons such th there is a double bond, R 3 is hydrogen, Rj and R^ are taken together to form a second bo between C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4-methoxybenzyl, and R, a R 8 are taken together to form the didepsipeptide with the strucmre X where R, is methyl and R is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 28, is the following CRYPTOPHYCIN-28 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between the C 10 and C„ carbons such th there is a double bond, R 3 is methyl, R 5 and -R^ are taken together to form a second bond betwee C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4-methoxybenzyl, and R, and R 8 ar taken together to form the didepsipeptide with the strucmre X where R, is hydrogen and R, 0 i isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 29, is the following: CRYPTOPHYCIN-29 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between the C 10 and C„ carbons such tha there is a double bond, R 3 is methyl, R 5 is hydroxy, R^ is hydrogen, R 7 is 3-chloro-4 methoxybenzyl, and R 4 and R 8 are taken together to form the didepsipeptide with the strucmr X where R, is methyl and R 10 is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 30, is the following: CRYPTOPHYCIN-30 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form an epoxide group, R 3 is methyl, It, and R « are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3,5- dichloro-4-methoxy benzyl, and R 4 and R 8 are taken together to form the didepsipeptide with the structure X where R, is methyl and R 10 is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 31, is the following: CRYPTOPHYCIN-31 A further example of a novel cryptophycin compound of the present invention is when R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 is hydrogen, R « is hydrogen, R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the didepsipeptide with the strucmre X where -R, is methyl and R 10 is isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 35, is the following: CRYPTCPHYCIN-35 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form an epoxide group, R 3 is hydrogen, R j and ^ are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is chloro-methoxybenzyl, and R 4 and R 8 are taken together to form the didepsipeptide with t strucmre X where R, is methyl and R 10 is isobutyl. The strucmre of this cryptophycin compoun Cryptophycin 40, is the following: CRYPTOPHYCIN-40 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between the C 10 and C„ carbons such tha there is a double bond, R 3 is methyl, - j and R^ are taken together to form a second bond betwee C 5 and C 6 such that there is a double bond, R 7 is 3,5-dichloro-4-hydroxybenzyl, and R 4 and are taken together to form the didepsipeptide with the strucmre X where -R, is methyl and R 10 i isobutyl. The strucmre of this cryptophycin compound, Cryptophycin 45, is the following: CRYPTOPHYCIN-45 A further example of a novel cryptophycin compound of the present invention is whe R, and R, are taken together to form an epoxide group, R 3 is methyl, R^ and R < , are take together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3 chloro-4-methoxybenzyl, and R, and R 8 are taken together to form the didepsipeptide with th strucmre X where -R, is methyl and R I0 is propyl. The strucmre of this cryptophycin compound, Cryptophycin 49, is the following: CRYPTOPHYCIN-49 A further example of a novel cryptophycin compound of the present invention is whe R, and R 2 are taken together to form a second bond between the C 10 and C„ carbons such tha there is a double bond, R 3 is methyl, R 5 and R 6 are taken together to form a second bond betwee C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 ar taken together to form the didepsipeptide with the strucmre X where R, is methyl and R 10 i propyl. The strucmre of this cryptophycin compound, Cryptophycin 50, is the following: CRYPTOPHYCIN-50 A further example of a novel cryptophycin compound of the present invention is when R, and R, are taken together to form an epoxide group, R 3 is methyl, R, and R^ are taken together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3- chloro-4-methoxy benzyl, and R 4 and R 8 are taken together to form the didepsipeptide with the strucmre X where -R, is methyl and R 10 is sec-butyl. The strucmre of this cryptophycin compound, Cryptophycin 54, is the following: CRYPTOPHYCIN-54 Of the above compounds, Cryptophycins 2, 4, 16-19, 21, 23, 24, 26, 28-31, 40, 43, 4 49, 50, and 54 are metabolites produced by a strain of Nostoc sp. of blue-green alg (cyanobacteria) which has been cultured, with these compounds subsequently isolated from th culture. Cryptophycins 6 and 7 are artifacts that are produced if the isolation procedure utiliz solvents containing methanol. Cryptophycins 8, 9, 10-12, 14, and 35 are derivatives of thes namrally-produced metabolites, having been chemically modified with the methods described i the Experimental Section of this application, with alternate methods to create the exemplifie compounds, as well as the non-exemplified compounds, available to those of ordinary skill in th art. The present invention provides methods of producing the above cryptophycin compound through the culturing of a strain of the Nostoc sp. The morphological characteristics of th Nostoc sp. of blue-green algae (cyanobacteria), as provided in U.S. Patent No. 4,946,835, ar that they are filamentous and consist of vegetative cells. In longer filaments, heterocyst occasionally are observed in an intercalary position. Akinetes are not observed. Reproductio is by hormogonia in addition to random trichome breakage. The basis for an identification o a Nostoc sp. can be found in J. Gen. Micro. , 111 : 1-61 (1979). The invention further provides that a Nostoc sp. may be cultured and that nove cryptophycin metabolites, as well as previously disclosed cryptophycin metabolites, may b isolated from this culture. In a preferred embodiment of the present invention, the Nostoc sp strain designated GSV 224 is the strain which is cultivated and from which are isolate compounds represented by the following strucmre: Wherein R, is H, OH, a halogen, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; o R, and R, may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or second bond between C 10 and C„; or R, and R 4 may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R, is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; Rj is H or an OH group; R e is H; or R- 5 and R^ may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxy benzyl, methoxy benzyl, halohydroxybenzyl, dihalohydroxybenzy halomethoxy benzyl, or dihalomethoxy benzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterifi lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R, and R 8 may be taken together to form a didepsipeptide group consisting of a lower β-ami acid bonded to a lower α-hydroxy alkanoic acid; or Rj and R 8 may be taken together to form a didepsipeptide group consisting of a lower β-ami acid bonded to a lower α-hydroxy alkanoic acid; with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone grou NH 2 , SH. In a preferred embodiment of the invention, chemically modifying a cryptophyci metabolite isolated by the above method provides a distinct compound also having this strucmr Procedures for chemically modifying cryptophycin compounds to produce additional compoun within the scope of the present invention are available to those of ordinary skill in the ar Moreover, additional procedures are described in greater detail in the Experimental Section this application. In addition to the novel cryptophycin compounds of the present invention, the prese invention provides novel methods of producing, as well as using, the above strucmre whic includes the following previously disclosed cryptophycin species, Cryptophycins 1, 3, 5, 13 an 15. The structures of these compounds are the following: CRYPTOPHYCIN 1 CRYPTOPHYCIN 3 15 20 CRYPTOPHYCIN 5 25 30 CRYPTOPHYCIN 13 CRYPTOPHYCIN 15 The invention provided herewith is directed to any strain of the Nostoc sp. and preferabl to the Nostoc sp. GSV 224 strain to produce cryptophycin compounds. To that end, the GS 224 strain of Nostoc sp. was deposited on October 7, 1993 pursuant to the Budapest Treaty o the International Deposit of Microorganisms for the Purposes of Patent Procedure with the Paten Culture Depository of the American Type Culture Collection, 12301 Parklawn Drive, Rockville Maryland 20852 U.S.A. under ATCC Accession No. 55483. Other strains of Nostoc sp. , i particular strain MB 5357 previously deposited by Merck and Co. under ATCC accession No. 53789, are strains contemplated to be utilized to practice the present invention. As is the case with other organisms, the characteristics of Nostoc sp. are subject t variation. For example, recombinants, variants, or mutants of the specified strains may be obtained by treatment with various known physical and chemical mutagens, such as ultraviole ray, X-rays, gamma rays, and N-methyl-N'-nitro-N-nitrosoguanidine. All natural and induced variants, mutants, and recombinants of the specified strains which retain the characteristic o producing a cryptophycin compound are intended to be within the scope of the claimed invention. The cryptophycin compounds of the present invention can be prepared by culturing a strain of Nostoc sp. under submerged aerobic conditions in a suitable culture medium until substantial antibiotic activity is produced. Other culture techniques, such as surface growth on solidified media, can also be used to produce these compounds. The culture medium used to grow the specified strains can include any of one of many nitrogen and carbon sources and inorganic salts that are known to those of ordinary skill in the art. Economy in production, optimal yields, and ease of product isolation are factors to consider when choosing the carbon sources and nitrogen sources to be used. Among the nutrient inorganic salts which can be incorporated in the culture media are the customary soluble salts capable of yielding iron, potassium, sodium, magnesium, calcium, ammonium, chloride, carbonate, phosphate, sulfate, nitrate, and like ions. Essential trace elements which are necessary for the growth and development of th organisms should also be included in the culture medium. Such trace elements commonly occu as impurities in other constiments of the medium in amounts sufficient to meet the growt requirements of the organisms. It may be desirable to add small amounts (i.e. 0.2mL/L) of a antifoam agent such as polypropylene glycol (M.W. about 2000) to large scale cultivation medi if foaming becomes a problem. For production of substantial quantities of the cryptophycin compounds, submerge aerobic cultivation in tanks can be used. Small quantities may be obtained by shake-flask culture Because of the time lag in metabolite production commonly associated with inoculation of larg tanks with the organisms, it is preferable to use a vegetative inoculum. The vegetative inoculu is prepared by inoculating a small volume of culture medium with fragments of the vegetativ trichome or heterocyst-containing form of the organism to obtain a fresh, actively growin culture of the organism. The vegetative inoculum is then transferred to a larger tank. Th medium used for the vegetative inoculum can be the same as that used for larger cultivations o fermentation, but other media can also be used. The organisms may be grown at temperatures between about 20 °C and 30 °C and a incident illumination intensity of about 100 to 200μmol photons m 2 Sec " ' (photosynthetically active radiation). As is customary in aerobic submerged culture processes of this type, carbon dioxide gas is introduced into the culture by addition to the sterile air stream bubbled through the culture medium. For efficient production cf the cryptophycin compounds, the proportion of carbon dioxide should be about 1 % (at 24 °C and one atmosphere of pressure). The prior art, specifically U.S. Patent No. 4,946,835, provides methods of cultivating Nostoc sp., the contents of which are hereby incorporated by reference. Cryptophycin compound production can be followed during the cultivation by testing samples of the broth against organisms known to be sensitive to these antibiotics. One useful assay organism is Candida albicans. Following their production under submerged aerobic culture conditions, cryptophycin compounds of the invention can be recovered from the culture and from the culture media by methods known to those of ordinary skill in this art. Recovery is generally accomplished by initially filtering the culture medium to separate the algal cells and then freeze-drying the separated cells. The freeze-dried alga can be extracted with a suitable solvent such as ethanol, methanol, isopropanol, or dichloromethane. The cryptophycins can be separated by subjecting this extract, as well as the culture media, to rapid chromatography on reversed-phase colum The cryptophycins can be purified by reversed-phase high-performance liquid chromatograph (HPLC). As will be apparent from their structures, the cryptophycin compounds have groups whic are capable of chemical modification. The genus compound of the present inventio contemplates those cryptophycins which exhibit anti-neoplastic activity. For example, th derivatives exemplified in the present invention include compounds having the epoxide oxyge or hydroxy groups on C-7 and C-8 of unit A or the leucic acid group of unit B of Figure 1 Such derivatives of the novel and previously disclosed compounds which display the desired anti neoplastic activity are included in the claimed invention. Moreover, the relationship between th strucmre of the cryptophycin compounds and anti-neoplastic activity is provided in th Experimental Section hereinbelow. While selected cryptophycin compounds are known to be metabolites produced by the alg of the present invention, other cryptophycin compounds, e.g. Cryptophycins 8-15, can be derive from the metabolites using published techniques which are known to those of ordinary skill i the art; for example, the syntheses disclosed in U.S. Patent Nos. 4,868,208, 4,845,086, an 4,845,085, the contents of which are hereby incorporated by reference, or by utilizing othe methods which are known to those of ordinary skill in the art. Moreover, the present inventio provides methods of producing derivatives in the Experimental Section. The novel cryptophycin compounds of the present invention and the previously disclose cryptophycin compounds can be therapeutically employed as anti-neoplastic agents and thereb used in methods to treat neoplastic diseases. As used herein, "neoplastic" pertains to a neoplasm, which is an abnormal growth, such growth occurring because of a proliferation of cells no subject to the usual limitations of growth. As used herein, "anti-neoplastic agent" is an compound, composition, admixture, co-mixture or blend which inhibits, eliminates, retards o reverses the neoplastic phenotype of a cell. Chemotherapy, surgery, radiation therapy, therapy with biologic response modifiers, and immunotherapy are currently used in the treatment of cancer. Each mode of therapy has specific indications which are known to those of ordinary skill in the art, and one or all may be employed in an attempt to achieve total destruction of neoplastic cells. Chemotherapy utilizing one or more cryptophycins is provided by the present invention. Moreover, combination chemotherapy, chemotherapy utilizing cryptophycins in combination with other neoplastic agents, is also provided by the subject invention as combination therapy is generally more effective than the us of single anti-neoplastic agents. Thus, a further aspect of the present invention provide compositions containing a therapeutically effective amount of at least one new cryptophyci compound of the present invention, including nontoxic addition salts thereof, which serve t provide the above-recited therapeutic benefits. Such compositions can also be provided togethe with physiologically tolerable liquid, gel or solid carriers, diluents, adjuvants and excipients Such carriers, diluents, adjuvants and excipients may be found in the United States Pharmacopei Vol. XXII and National Formulary Vol XVII. U.S. Pharmacopeia Convention, Inc., Rockville MD (1989), the contents of which are herein incorporated by reference. Additional modes o treatment are provided in AHFS Drug Information. 1993 ed. by the American Hospita Formulary Service, pp. 522-660, the contents of which are herein incorporated by reference. The present invention further provides that the pharmaceutical composition used to trea neoplastic disease contains at least one cryptophycin compound and at least one additional anti neoplastic agent. Anti-neoplastic compounds which may be utilized in combination wit cryptophycin include those provided in The Merck Index. 11th ed. Merck & Co. , Inc. (1989 pp. Ther 16-17, the contents of which are hereby incorporated by reference. In a furthe embodiment of the invention, anti-neoplastic agents may be antimetabolites which may include, but are not limited to, methotrexate, 5-fluorouracil, 6-mercaptopurine, cytosine arabinoside, hydroxyurea, and 2-chlorodeoxyadenosine. In another embodiment of the present invention, the anti-neoplastic agents contemplated are alkylating agents which may include, but are not limited to, cyclophosphamide, melphalan, busulfan, paraplatin, chlorambucil, and nitrogen mustard. In a further embodiment of the subject invention, the anti-neoplastic agents are plant alkaloids which may include, but are not limited to, vincristine, vinblastine, taxol, and etoposide. In a further embodiment of the present invention, the anti-neoplastic agents contemplated are antibiotics which may include, but are not limited to, doxorubicin (adriamycin), daunorubicin, mitomycin c, and bleomycin. In a further embodiment of the subject invention, the anti- neoplastic agents contemplated are hormones which may include, but are not limited to, calusterone, diomostavolone, propionate, epitiostanol, mepitiostane, testolactone, tamoxifen, polyestradiol phosphate, megesterol acetate, flutamide, nilutamide, and trilotane. In a further embodiment of the subject invention, the anti-neoplastic agents contemplated include enzymes which may include, but are not limited to, L-Asparaginase or aminoacridine derivatives which may include, but are not limited to, amsacrine. Additional anti-neoplastic agents include those provided in Skeel, Roland T. , "Antineoplastic Drugs and Biologic Response Modifier: Classification, Use and Toxicity of Clinically Useful Agents, " Handbook of Cancer Chemotherapy (3rd ed.), Little Brown & Co. (1991), the contents of which are herei incorporated by reference. These compounds and compositions can be administered to mammals for veterinary use such as for domestic animals, and clinical use in humans in a manner similar to other therapeuti agents. In general, the dosage required for therapeutic efficacy will vary according to the typ of use and mode of administration, as well as the particularized requirements of individual hosts. Ordinarily, dosages will range from about 0.001 to iOOOmg/kg, more usually 0.01 to lOmg/kg, of the host body weight. Alternatively, dosages within these ranges can be administered b constant infusion over an extended period of time, usually exceeding 24 hours, until the desire therapeutic benefits have been obtained. Indeed, drug dosage, as well as route of administration, must be selected on the basis of relative effectiveness, relative toxicity, growth characteristics o tumor and effect of cryptophycins on cell cycle, drug pharmacokinetics, age sex, physical condition of the patient, and prior treatment. The cryptophycin compounds, with or without additional anti-neoplastic agents, may be formulated into therapeutic compositions as natural or salt forms. Pharmaceutically acceptable non-toxic salts include the base addition salts (formed with free carboxyl or other anionic groups) which may be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like. Such salts may also be formed as acid addition salts with any free cationic groups and will generally be formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or organic acids such as acetic, oxalic, tartaric, mandelic, and the like. Additional excipients which the further invention provides are those available to one of ordinary skill in the art, for example, that found in the United States Pharmacopeia Vol. XXII and National Formulary Vol XVII. U.S. Pharmacopeia Convention, Inc. , Rockville, MD (1989), which is herein incorporated by reference. The suitability of particular carriers for inclusion in a given therapeutic composition depends on the preferred route of administration. For example, anti-neoplastic compositions may be formulated for oral administration. Such compositions are typically prepared either as liquid solution or suspensions, or in solid forms. Oral formulations usually include such normally employed additives such as binders, fillers, carriers, preservatives, stabilizing agents, emulsifiers, buffers and excipients as, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, magnesium carbonate, and the like. These de¬ compositions take the form of solutions, suspensions, tablets, pills, capsules, sustained releas formulations, or powders, and typically contain l %-95% of active ingredient, preferably 2% 70% . Compositions of the present invention may also be prepared as injectable, either as liqui solutions, suspensions, or emulsions; solid forms suitable for solution in, or suspension in, liqui prior to injection may be prepared. Such injectables may be administered subcutaneously intravenously, intraperitoneally, intramuscularly, intrathecally, or intrapleurally. The activ ingredient or ingredients are often mixed with diluents or excipients which are physiologicall tolerable and compatible with the active ingredient(s). Suitable diluents and excipients are, fo example, water, saline, dextrose, glycerol, or the like, and combinations thereof. In addition if desired, the compositions may contain minor amounts of auxiliary substances such as wettin or emulsifying agents, stabilizing or pH buffering agents. The invention further provides methods for using cryptophycin compounds encompasse by the genus strucmre to inhibit the proliferation of mammalian cells by contacting these cell with a cryptophycin compound in an amount sufficient to inhibit the proliferation of th mammalian cell. A preferred embodiment is a method to inhibit the proliferation o hyperproliferative mammalian cells. For purposes of this invention, "hyperproliferative mammalian cells" are mammalian cells which are not subject to the characteristic limitations o growth, e.g. , programmed cell death ' (apoptosis). A further preferred embodiment is when the mammalian cell is human. The invention further provides contacting the mammalian cell with at least one cryptophycin compound and at least one additional anti-neoplastic agent. The types of anti-neoplastic agents contemplated are the same as those disclosed hereinabove. The invention further provides methods for using cryptophycin compounds encompassed by the genus strucmre to inhibit the proliferation of hyperproliferative cells with drug-resistant phenotypes, including those with multiple drug-resistant phenotypes, by contacting said cell with a cryptophycin compound in an amount sufficient to inhibit the proliferation of a hyperproliferative mammalian cell. A preferred embodiment is when the mammalian cell is human. The invention further provides contacting the mammalian cell with a cryptophycin compound and at least one additional anti-neoplastic agent. The types of anti-neoplastic agents contemplated are the same as those disclosed hereinabove. • The invention further provides a method for alleviating pathological conditions caused by hyperproliferating mammalian cells, for example, neoplasia, by administering to a subject an effective amount of a pharmaceutical composition provided hereinabove to inhibit the proliferation of the hyperproliferating cells. As used herein "pathological condition" refers t any pathology arising from the proliferation of mammalian cells that are not subject to the norma limitations of cell growth. Such proliferation of cells may be due to neoplasms, including, bu not limited to the following neoplasms: mammary, small-cell lung, non-small-cell lung colorectal, leukemia, melanoma, central nervous system (CNS), ovarian, prostate, sarcoma o soft tissue or bone, head and neck, gastric which includes pancreatic and esophageal, stomach myeloma, bladder, renal, neuroendocrine which includes thyroid and lymphoma, non-Hodgkin' and Hodgkin's. In a further embodiment of the invention, the neoplastic cells are human. Th present invention further provides methods of alleviating such pathological conditions utilizin cryptophycin in combination with other therapies, as well as other anti-neoplastic agents. Suc therapies and their appropriateness for different neoplasia may be found in Cancer Principles and Practice of Oncology, 4th ed.. Editors DeVita, V., Hellman, S. , and Rosenberg. , S. , Lippincott Co. (1993), the contents of which are herein incorporated by reference. In the present disclosure, cryplophycii; compounds are shown to potently disrupt the microtubule strucmre in cultured cells. In addition, and in contrast with the Vinca alkaloids, cryptophycin compounds appear to be a poor substrate for the drug-efflux pump P-glycoprotein. Cryptophycin 1 is the major cytotoxin in the blue-green alga (cyanobacteria) Nostoc sp. strain designated GSV 224 and shows excellent activity against tumors implanted in mice. This cyclic didepsipeptide had previously been isolated from Nostoc sp. ATCC accession no. 53787 as an antifungal agent and its gross strucmre was previously determined. The relative and absolute stereochemistry of this potentially important drag has now been established using a combination of chemical and spectral techniques. Twenty-four additional cryptophycin compounds, Cryptophycins 2-7, 16-19, 21, 23, 24, 26, 28-31, 40, 43, 45, 49, 50 and 54 have also been isolated from GSV 224 and their total structures and cytotoxicities determined. Several derivatives and degradation products are described, both chemically and pharmacologically. The following examples serve to illustrate certain preferred embodiments and aspects of the present invention and are not to be construed as limiting the scope thereof. Experimental Section In the experimental disclosure which follows, all weights are given in grams (g), milligrams (mg), micrograms (mg), nanograms (ng), picograms (pg) or moles (mol), all concentrations are given as percent by volume (%), molar (M), millimolar (mM), micromolar (μM), nanomolar (nM), or picomolar (pM), normal (N) and all volumes are given in liters (L), milliliters (mL) or microliters (μL), and measures in millimeters (mm), unless otherwis indicated. The following examples demonstrate the isolation and synthesis of cryptophyci compounds as well as their use as therapeutic agents in accordance with the invention. In screening extracts of over 1000 blue-green algae (cyanobacteria) for antitumor activity the lipophilic extract of Nostoc sp. GSV 224 was found to be strongly cytotoxic, 3 exhibitin minimum inhibitory concentrations (MICs) of 0.24ng/mL against KB, a human nasopharynge carcinoma cell line, and 6ng/mL against LoVo, a human colorectal adenocarcinoma cell line More importantly, this extract showed significant tumor selective cytotoxicity in the Corbe assay. 4,5 Bioassay monitored reversed-phase chromatography of the algal extract led to a fractio which was predominantly Cryptophycin 1, a potent fungicide that had been isolated earlier fro Nostoc sp. ATCC 53789 by researchers at Merck 67 and found to be very active against strain of Cryptococcus. Cryptophycin 1 accounted for most of the cytotoxic activity of the crude algal extract o Nostoc sp. GSV 224 and the pure compound showed IC 50 values of 3 and 5pg/mL against K and LoVo, respectively. In the Corbett assay Cryptophycin 1 was found to be strongly tumo selective and equally cytotoxic against drug-sensitive and drug-resistant tumor cells Immunofluorescence assays showed that Cryptophycin 1 interact with a cellular target similar t that of vinblastine, but differed from the latter drug in having a longer time course of action an in not forming paracrystalline bodies. In preliminary in vivo experiments, Cryptophycin exhibited very promising activity against tumors implanted in mice. Minor amounts of several other cryptophycin compounds were present in Nostoc sp. GS 224. Twenty-one of these could be isolated in sufficient quantities for strucmre determination and antitumor evaluation in vitro by extraction of the alga with 1 : 5 dichloromethane/acetonitril and reversed-phase HPLC of the extract. Cryptophycins 2, 3, 4, 16, 17, 18, 19, 21, 23, 24, 26, 28, 29, 30, 31, 40, 43, 45, 49, 50 and 54 accompanied Cryptophycin 1 in the fraction elute from a reversed-phase flash column with 65:35 ace tonitrile/ water. Cryptophycins 2, 3, 4, 5, 6, and 7 were the only compounds found when the alga was extracted with methanol and the reversed-phase chromatography was carried out with mεthanυl /water. Cryptophycins 2, 3, 4, 5 and 6 were eluted with 3: 1 methanol/water and Cryptophycin 7 was found in an earlier, less cytotoxic fraction eluted with 1:3 methanol/water. Acyclic Cryptophycins 5, 6 and 7 appear to be artifacts generated by decomposition of Cryptophycin 1 during the isolation procedure. Cryptophycins 3 and 5 appeared to be identical with fungicidal semi-synthetic compound prepared from Cryptophycin 1 by researchers at Merck. 89 Cryptophycin 3 was prepared b treating Cryptophycin 1 with a zinc-copper couple or with diphosphorus tetraiodide. Cryptophycin 5 was prepared by methanolysis of Cryptophycin l. 9 Example 1 Strucmre Determination The determination of the structures of the new compounds, viz. Cryptophycins 2, 4, 6 7, 8, 9, 10, 12, 14, 16, 17, 18, 19, 21, 23, 24, 26, 28, 29, 30, 31, 40, 43, 45, 49, 50 and 54, as well as those previously disclosed, were carried out in a straightforward manner using methodology that is well-known to those trained in the art. Mass spectral data were consistent with the molecular compositions. Proton and carbon NMR data obtained from COSY, HMQC, HMBC and NOESY spectra allowed one to assemble all of the gross structures of these depsipeptide-type compounds. The presence of the various hydroxy and amino acid units in each compound were confirmed by gas chromatographic mass spectral analysis. Total structures, including absolute stereochemistries, were determined using a combination of chemical degradative and special analytical techniques on appropriate derivatives of the cryptophycin compounds. Example 2 Structure-Activity Relationships (SAR) To probe the structural features in Cryptophycin 1 needed for optimal activity, all of the compounds described herein were evaluated for cytotoxicity against KB (human nasopharyngeal carcinoma), LoVo (human colon carcinoma), and SKOV3 (human ovarian carcinoma) cell lines. IC 50 values are listed in Tables 1 and 2. Comparison of the cytotoxicities show that the intact macrolide ring, the epoxy and methyl groups and the double bond in the 7,8-epoxy-5-hydroxy-6- methyl-8-phenyl-2-octenoic acid unit (see Unit A in Figure 1), the chloro and O-methyl groups in the 3-(3-chloro-4-methoxyphenyl)alanine unit (Unit B), the methyl group in the 3-amino-2- methylpropionic acid unit (Unit C), and the isobutyl group in the leucic acid unit (Unit D) of Cryptophycin 1 are needed for optimal cytotoxicity. The potent cytotoxicity of Cryptophycin 8 is most likely due to the chlorohydrin functionality which acts as a masked epoxide. The most active compounds were also evaluated for selective cytotoxicity against four different cell types, viz. a murine leukemia (L1210 or P388), a murine solid tumor (colon adenocarcinoma 38 , pancreatic ductal ad enocarcinoma 03 , mammary adenocarcinoma M 16/M 17) , a human solid tumor (colon CX-1, HCT8, HI 16; lung H125; mammary MX-1, MCF-7), and a low malignancy fibroblast (LML), using the Corbett assay.2 a disk diffusion assay modele after the one commonly used in antifungal and antibacterial testing. The results, shown in Tabl 1, indicated that Cryptophycins 1-5 and 8 were neither solid tumor nor leukemia selective, bu rather equally active against mmor cell lines, including drag-resistant ones such as Ml 7. Non of the compounds showed a zone of inhibition for any of the solid mmor cell lines that was 3 25 zone units, i.e. 3 7.5mm, larger than the zone of inhibition for the leukemia cell line. Cryptophycins 1-5 and 8, however, displayed markedly larger zones of inhibition ( 3 400 zon units) for all of the tumor cell lines compared with the zone of inhibition for the fibroblast LML. Diagnostically LML has been found to behave more like a normal cell than a tumor cell wit respect to clinically-useful cytotoxic agents (see Corbett assay data for 5-fluorouracil, etoposide and taxol in Table 1). Since the differential cytotoxicities were >250 zone units, Cryptophycins 1-5 and 8 were mmor selective. These compounds therefore became candidates for in vivo testing. Cryptophycin 1 is active against a broad spectrum of murine and human tumors implanted in mice, including drug-resistant ones (Table 3). It exhibits excellent activity against five early stage murine tumors, viz. colon adenocarcinomas #38 and #51, taxol-sensitive and taxol-resistant mammary #16/C/RP, and pancreatic ductal adenocarcinoma #03, and two early stage human tumors tested in SCID mice, viz. MX-1 breast and HI 25 adenosquamous lung, showing mmor burden T/C (mean mmor burden in treated animals/mean mmor burden untreated animals) values that are less than 10% . T/C values that are less than 42% are considered to be active by NCI standards; T/C values that are less than 10% are considered to have excellent activity and potential clinical activity by NCI standards. 9 Two of the trials showed gross (mmor cell) log kill values of 2.0. Gross log kill is defined as T-C/3.2 Td where T is the median time in days for the tumors of the treated group to reach 750 mg, C is the median time in days for the tumors of the control group to reach 750 mg, and Td is the mmor volume doubling time. Gross log kill values of > 2.8, 2.0-2.8, 1.3-1.9, 0.5-0.8, and <0.5 with duration of drug treatment of 5-20 days are scored + + + + , + + + , + + , + and - (inactive), respectively. An activity rating of + + + to --- + + + , which is indicative of clinical activity, is needed to effect partial or complete regression of 100-300 mg size masses of most transplanted solid tumors of mice. Cryptophycin 8 is also active against a broad spectrum of tumors implanted in mice (Table 4). It has shown excellent activity against all of the tumors tested to date, showing mmor burden T/C values < 10%, but more importantly gross log kill activity ratings of + + + t + + + + and some cures. Good in vivo activity was also seen with Cryptophycin 35 in the one trial that has bee ran to date. Lethal toxicity observed during testing of Cryptophycins 1 and 8 was attributed to leucopenia which is common to all clinically used antitumor drugs. Table 1. Cytotoxicity data for cryptophycins and semi-synthetic analogs. Corbett/Valeriote assay data for 5-fluorouracil, etoposide (VP-16) and taxol are included for comparison. Type of Cytotoxicity (Differential in Zone Units) Corbett Valeriote KB IC 50 LoVo IC 50 Compound μg/disk Assay 2 μg/disk Assay 6 ng/mL ng/mL 1 12.5 E/T( >400) c N 0.005 0.003 2 25 E/T( >400) c N 0.007 0.0002 3 25 E/T( >400) c N 0.3 0.5 4 20 E/T( > 400) c N 1.3 0.5 5 2.9 E/T( > 600) c N 0.02 0.02 6 250 I ≥IOO ≥ IOO 7 ≥750 ≥480 8 30 E/T( >500) c 30 N 0.0002 0.01 9 15 Not Deter¬ mined 10 ≥IOO ≥IOO 12 ≥IOO ≥IOO 14 1.8 3 5-FU 2.5 M/T( >400) d 2.5 LL( >400) VP-16 5 L(350),T(530) d 5 LL(260) taxol 0.2 M/H/T(≥400) d a L= leukemia selective (e.g. Z L]2 , 0 -Z C38 and Z L1210 - Z H8 ≥250zu) M = murine solid tumor selective (e.g. Z C38 - Z LI210 ≥250 zu) H = human solid selective (e.g. Z H8 - Z L1210 ≥250 zu) E= equally cytotoxic towards leukemia and solid tumor cell lines (inhibition zones ≥250 zu) T= tumor selective (e.g. Z L1210 - Z LML ,Z C38 - Z LML , and Z H8 - Z LML ≥250 zu I = inactive (inhibition zones < 250) b N = non-selective towards tumor (leukemia) and normal cell (CFU-GM) lines LL= lymphocytic leukemia selective (Z 210 - Z CFU GM ≥250 zu) ML= acute myelogenous leukemia (AML) selective (Z AML - Z CFU . GM ≥250 zu). Selective against drug-sensitive and drug-resistant cell lines (Z C38 - Z LML ,Z M17 - Selective against drug -sensitive cell lines only. Table 2. In Vitro Cytotoxicity Data of Cryptophycins Cryptophycin KBI o LoVOI o SKOV3IC 50 ng/mL ng/mL ng/mL 1 0.0025 0.001 0.026 10 2 0.023 0.021 0.18 3 1.8 0.6 2.8 4 6 2.5 21 5 12 2 7.4 8 0.01 0.0022 0.15 15 12 18 3 15 12 16 0.08 0.02 0.64 17 4.7 5.9 11 18 15 4.5 23 20 19 9.8 5.9 41 21 0.01 0.0003 0.029 23 0.89 0.4 1.7 24 0.12 0.095 0.3 26 19 9.8 95 25 28 1.5 0.75 6.1 29 1 0.49 3.4 30 11 8 21 31 0.53 0.062 1.9 35 0.055 0.01 0.092 30 40 9.0 1.0 1.7 43 0.72 0.8 1.1 45 2.3 2.4 1.6 49 1.4 1.9 1.1 50 0.17 0.17 0.2 35 54 0.80 2.2 2.2 Table 3. In Vivo Activity of Cryptophycin-A mg/kg % Body # of Inj. Total Wt. Loss Exp # SC Tumor IV Dose at Nadir T/C Log Cures Kill 1560 Colon 38 8 10.3 Gain 6% 1.5 0/5 1694 Pane 03 8 16.0 Gain 0% 2.0 0/5 1636 Colon 51 7 28.1 -11 % 7% 1.3 0/5 1720 Mam 16/C 5 13.2 -1 % 5 % 1.4 0/5 1733 Mam 16/Taxol 5 16.5 0% 2% 1.8 0/4 1833 M17/0 (Adr. Sens.) 5 5.4 -10% 23 % < 1 0/5 1749 Pane 02 5 11.0 -5% 20% 1.1 0/5 1596 Human Sm Cell L. 6 7.3 0% 27% < 1 0/5 DMS273 SCID 1806 MX-l Human Breast 8 12 -3% 3% 2.0 0/5 1823 H125 Human 8 14.4 -15% 9% 1.1 0/5 Adenosq-lung 1/5 dead 1841 LNCaP Human 6 6.5 -6% 26% < 1 0/5 Prostate Table 4. In Vivo Activity of Cryptophycin Analogs # of % Inj. mg/kg Body Exp # Agent SC IV TD Wt. T/C Log Cures Tumor Loss at Kill Nadir 1813 Cryptophycin-2 P03 10 37 -2% 44% < 1 0/5 1843 Cryptophycin-3 P03 4 28/5 -9% 54% < 1 0/5 1769 Cryptophycin-8 C38 15 45 -2% > 100 None 0/5 % 1825 Cryptophycin-8 P03 11 106 -6% 4% 4.6 0/5 1885 Cryptophycin-8 Mam 7 21.3 -4.5% 6% 2.5 0/5 16/C 1887B Cryptophycin-8 C38 6 30 -2% 0% 2.8 1/5 1900 Cryptophycin-8 Colon 9 67.5 -1 % 7% 1.8 0/5 51 1843 Cryptophycin- 15 P03 5 18 -7% 83 % None 0/5 1878 Cryptophycin- 16 P03 9 82 -1 % 89% None 0/5 1813 Cryptophycin-21 P03 9 27 -11 % 61 % None 0/5 (1/5 dead) 1843 Cryptophycin-35 P03 7 23 -2% 11 % 1.3 0/5 Example 3 Culmre Conditions Nostoc sp. GSV 224 was obtained from Professor C. P. Wolk, MSU-DOE Plant Research Laboratory, Michigan State University. Nostoc sp. ATCC 53789 was purchased from the American Type Culmre Collection. A IL flask culmre of alga was used to inoculate an autoclaved 20L glass carboy containing an inorganic medium, designated modified BG-11 3 , the pH of which had been adjusted to 7.0 with NaOH. Culmres were continuously illuminated at an incident intensity of 200μmol photons m 2 sec " ' (photosynthetically active radiation) from banks of cool-white fluorescent tubes and aerated at a rate of 5L/min with a mixture of 0.5% CO 2 in air at a temperature of 24 4- 1 °C. Typically, the culture was harvested by filtration after 21 days. The yields of lyophilized Nostoc sp. GSV 224 and ATCC 53789 averaged 0.61 and 0.3g/L of culmre, respectively. Example 4 Isolation Method A The lyophilized Nostoc sp. GSV224 (50 g) was extracted with 2 L of 1 : 5 CH 2 C1 2 /CH 3 CN for 48 h and the extract concentrated in vacuo to give a dark green solid. The residue (1 g; KB MIC 0.24 ng/mL) was applied to an ODS-coated silica column (55 g, 7 x 5 cm) and subjected to flash chromatography with 1 :3 CH 3 CN/H 2 O (0.8 L), 1: 1 CH 3 CN/H 2 O (0.8 L), 65:35 CH 3 CN/H 2 O (1.0 L), MeOH (0.8 L), and CH 2 C1 2 (0.5 L). The fraction that was eluted with 65:35 CH 3 CN/H 2 O (420 mg; KB MIC 14 pg/mL) was subjected to reversed-phase HPLC (Econosil C18, 10μm, 25cm x 21.5mm, UV detection at 250nm, 65:35 CH 3 CN/H 2 O, flow rate 6 mL/min) to obtain Cryptophycin 1 (t R 49.3 min, 220 mg) and a number of impure fractions. The fraction eluted from the Econosil C18 column at t R 28.8 min was further purified by normal phase HPLC (Econosil silica 5m cartridge, 250 x 4.6mm, 6:4 ethyl acetate/hexane, 3 mL/min) to give Cryptophycin 16 (3.0 mg). The fraction eluted from the Econosil C18 column at t R 32.5 min was subjected to HPLC on the Econosil silica column using 55:45 ethyl acetate/hexane at 3 mL/min to give Cryptophycin 24 (0.8 mg). The fraction eluted from the Econosil C18 column at t R 35.5 min was subjected to HPLC twice on the Econosil silica column, first using 1 : 1 ethyl acetate/hexane at 3 mL/min and second using 4 : 6 ethyl acetate /methylene chloride at 2.5 mL/min to give Cryptophycin 23 (1.2 mg) and Cryptophycin 43 (O. lmg). The fraction eluted from the Econosil C18 column at t R 39.5 min was subjected to HPLC on the Econosil silica column with 1 : 1 ethyl acetate/hexane at 3 mL/min to give Cryptophycin 2 (6 mg) and Cryptophycin 21 (14 mg) and a complex mixmre of Cryptophycins eluted at t R 32.5 min. This latter fraction, accumulated from 400 gm dry alga, was chromatographed successively on a semi preparative column (partisil C18, 250 x 9.4mm, 10m) with 35:65 water/acetonitrile and a reversed phase analytical column (Econosil, 150 x 4.6mm, 5m) with 5 : 4 : 1 water/acetonitrile/methanol at 1.3 mL/min to give Cryptophycin 50 (t R 34.8, 0.4mg) and Cryptophycin 40 (t R 38.8 min, 0.3 mg). The fraction eluted from the Econosil C18 column at t R 44.5 min was subjected to HPLC on the Econosil silica column with 1 : 1 ethyl acetate/hexane at 3 mL/min to give Cryptophycin 17 (0.3 mg). Normal phase HPLC purification of the fraction eluted from the Econosil C18 column at t R 54.5 as a shoulder to Cryptophycin 1 yielded Cryptophycin 45 (t R 6.7 min, 0.1 mg), Cryptophycin 26 (t R 8.9 min, 0.5 mg), and Cryptophycin 54 (t R 19.8 min, <0.1 mg) on elution with 1 : 1 ethyl acetate/hexane. The fraction eluted from the Econosil C18 column as a broad peak (t u 58 to 70 min) was subjected to HPLC on the Econosil silica column with 43:57 ethyl acetate/hexane at 2.5mL/min to give Cryptophycin 4 (t R 19.6 min, 1.5 mg), Cryptophycin 31 (t R 9.4 min, 0.8mg), Cryptophycin 19 (t R 25.8min, 0.3mg), Cryptophycin 49 (t R 28 min, 0.1 mg), Cryptophycin 28 (t R 29.0min, 0.5 mg) and impure Cryptophycin 29 (t R 52.5 min, 2.0 mg) and Cryptophycin 30 (t R 49 min, 3.0 mg). Cryptophycins 29 and 30 obtained pure after reversed phase HPLC (Econosil C18, 10m, 250 x 10 mm, 3:1 methanol/water). The fraction eluted from the Econosil C18 column at t R 78.9 min was subjected to HPLC on the Econosil silica column with to give Cryptophycin 3 (t R 16.4 min, 3.0 mg). The fraction eluted from the Econosil C18 column at t R 82.8 min was subjected to HPLC on the Econosil silica column with 45 : 55 ethyl acetate/hexane at 3 mL/min to give Cryptophycin 18 (t R 19.2, 0.8mg). Method B The lyophilized Nostoc sp. GSV 224 (12.23g) was extracted twice with 700mL and 400mL portions of MeOH for 12 and 5 hours (h), respectively. The extracts were combined and concentrated in vacuo to give 1.84g of a dark green solid which was partitioned between water and CH 2 C1 2 . The lipophilic portion (0.65g; KB MIC 0.24ng/mL) was applied to an ODS-coated silica column (55g, 7 x 5cm) and subjected to flash chromatography with 1:3 MeOH/H 2 0 (0.8L), 1: 1 MeOH/H 2 O (0.8L), 3: 1 MeOH/H 2 0 (0.8L), MeOH (0.8L), and CH 2 C1 2 (0.5L). The fraction that was eluted with 3: 1 MeOH/H 2 O (22mg; KB MIC 14pg/mL), which accounted for essentially all of the cytotoxic activity, was subjected to reversed-phase HPLC (Econosil C18, lOμu, 250cm xlOmm, UV detection at 250nm, flow rate 3mL/min) using 1:5 MeOH/H,O as the eluant to give Cryptophycins 7 (t R 7.6 min, 0.2mg), 5 (t R 15.4 min, 2.3mg), 2 (t R 16.0 min, l.Omg), 1 (t R 19. min, 12.0mg), 4 (t R 26.5 min, 1.2mg), and 3 (t R 30.2 min, 1.4mg). From one of the culmre the fraction (8.1mg) that eluted from the flash column with 1:3 MeOH/H,O showed milde cytotoxicity (KB MIC 2μg/mL). Purification on HPLC using 2:3 MeOH/H 2 O as the eluan yielded cryptophycin G (7, t R 6.0 min, 2.4mg). Example 5 Spectral Data for Cryptophycins 1-7 The bold italicized letters in the spectral data refer to the units A-D in Figure 1. Cryptophycin 1 [α] D +33.8°(MeOH, c 1.83); UV λ max (e) 208 (42,400), 218 (33,700), 228 (23,800), 28 (2,210); CD [θ] 202 + 15,900, [θ] 206 +64,900, [θ] 214 +26,900, [θ] 224 +46,300, [θ] 237 + 10,500. IR (CHCl j ) v. ^ 3425, 2963, 1751, 1719, 1677, 1502, 1259 cm 1 . EIMS m/z (rel intensity) 654/656 (20/9), 412/414 (33/12), 280/282 (31/12), 227 (80), 195/197 (92/44), 91 (100); high resolution EIMS n/z 654.2665 (calcd for C 35 H 43 CIN 2 O 8 , 4.3 mmu error). Η NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 7 ,8-epoxy-5-hydroxy-6- methyl-8-phenyl-2-octenoic acid (A) 5.74 (2, dt; 15.5 and 0.9), 6.68 (3, ddd; 15.5, 9.6 and 5.2), 2.45 (4, ddd; 14.2, 11.1 and 9.6), 2.55 (4, brdd; 14.2 and 5.2), 5.16 (5, ddd; 11.1, 4.9 and 1.9), 1.80 (6, m), 1.14 (6-Me, d; 7.1), 2.92 (7, dd; 7.5 and 2.0), 3.69 (8, d; 2.0), 7.25 (10/14, m), 7.34-7.39 (11/12/13, m); leucic acid (D) 4.83 (2, dd; 6.8 and 3.3), 1.70 (3, m), 1.36 (3, m), 1.70(4, m), 0.86 (5, d; 6.6), 0.85 (5' , d; 6.6); 3-amino-2-methylpropionic acid (C) 2.71 (2, m), 1.22 (2-Me, d; 7.1), 3.30 (3, ddd; 13.4, 5.8 and 3.8), 3.48 (3, ddd; 13.4, 6.3 and 5.8), 6.93 (3-NH, brt; 5.8); S-chloro-4-methoxyphenylalanine (B) 4.80 (2, ddd; 8.7, 7.3 and 5.4), 5.61 (2-NH, d; 8.7), 3.03 (3, dd; 14.4 and 7.3), 3.13 (3, dd; 14.4 and 5.4), 7.21 (5, d; 2.1), 3.87 (7-OCH 3 ,s), 6.83 (8, d; 8.5), 7.07 (9, dd; 8.5 and 2.1). 13 C NMR (CDC1 3 ): unit δ (carbon position) ,4 165.3 (1), 125.3 (2), 141.0 (3), 36.7 (4), 76.2 (5), 40.6 (6), 13.5 (6-Me), 63.0 (7), 59.0 (8), 136.7 (9), 125.6 (10/14), 128.7 (11/13), 128.5 (12); D 170.7 (1), 71.3 (2), 39.4 (3), 24.5 (4), 22.9 (5), 21.3 (5'); C 175.6(1), 38.2 (2), 14.1 (2-Me), 41.1 (3); B 170.9 (1), 53.6 (2), 35.0 (3), 129.7 (4), 131.0 (5), 122.4 (6), 154.0 (7), 56.1 (7-OCH 3 ), 112.2 (8), 128.4 (9). Crvptophvcin 2 [α] D +20.4°(MeOH, c 0.54); UV λ m (έ) 206 (43,800), 218 (37,500), 232 (22,900), 278 (2,410); CD [θ] 203 +54,100, [θ] 212 + 16,500, [θ] 225 +53,600, [θ] 236 -14,000. IR (CHC1 3 ) v ^ 3423, 3029, 2961, 1742, 1724, 1678, 1512, 1258 cm 1 . EIMS m/z (rel intensity, assignment) 620 (11, M + ), 431 (3), 378(8), 377 (6), 311 (11), 246 (10), 244 (8), 227 (14), 195 (17), 161 (84, CH 3 O-C 6 H 4 -CH=CH=CO + ), 121 (79, CH 3 O-C 6 H 4 -CΗ 2 + ), 91 (1 0 0); high resolution EIMS m/z 620.3094 (calcd for C^N-O,. 0.3 mmu error); 161.0605 (calcd for C 10 H 9 O 2 , -0.2 mmu error); 121.0658 (calcd for C 8 H 9 O, -0.4 mmu error). 'H NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 7,8-epoxy-5-hydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.71 (2, dd; 15.4 and 1.3), 6.70 (3, ddd; 15.4, 10.2 and 5.0), 2.45 (4, m), 2.55 (4, m), 5.18 (5, ddd; 11.3, 4.8 and 2.0), 1.79 (6, m), 1.14 (6-Me, d; 7.0), 2.92 (7, dd; 7.7 and 2.0), 3.68 (8, d; 2.0), 7.24 (10/14, m), 7.34-7.39 (11/12/13, m); leucic acid (D) 4.82 (2, dd; 10.1 and 3.7), 1.70 (3, m), 1.33 (3, m), 1.70 (4, m), 0.86 (5, d; 6.4), 0.84 (5' , d; 6.4); 3-amino-2- methylpropionic acid (C) 2.68 (2, m), 1.23 (2-Me, d; 7.3), 3.39 (3-H 2 , m), 7.02 (3-NH,brt; 6.0); O-methyltyrosine (B) 4.79 (2, ddd; 8.1, 7.0 and 5.7), 5.55 (2-NH, d; 8.1), 3.07 (3, dd; 14.5 and 7.0), 3.13 (3, dd; 14.5 and 5.7), 7.10 (5/9, d; 8.6), 6.81 (6/8, d; 8.6), 3.78 (7-OCH 3 , s). 13 C NMR (CDC1 3 ): unit δ (carbon position) A 165.1 (1), 125.1 (2), 141.1 (3), 36.7 (4), 76.0 (5), 40.7(6), 13.6 (6-Me), 63.0 (7), 59.0 (8), 136.7 (9), 125.6 (10/14), 128.7 (11/13), 128.5 (12); D 170.6(1), 71.3 (2), 39.4 (3), 24.5 (4), 21.3 (5), 22.9 (5'); C 176.0 (1), 38.1 (2), 14.2 (2-Me), 40.7 (3); B 171.1 (1), 53.9 (2), 35.3 (3), 131.0 (4), 130.2 (5/9), 114.1 (6/8), 158.6 (7), 55.2 (7-OCH 3 ). Cryptophycin 3 [α] D +20.3°(MeOH, c 1. 13); UV λ^e) 206 (51,700), 218 (31,200), 230 (22,900), 246 (18,800), 280 (3,230); CD [θ] 205 +50,000, [θ] 212 -390, [θ] 218 -47,200, [θ] 233 -100, [Θ , +33,400, [θ] 271 +4,310. IR (CHC1 3 ) Vmax 3417, 2926, 1742, 1721, 1676, 1499, 1336 cm 1 . EIMS m/z (rel intensity) 638/640 (2/0.7, M + ), 412/414 (63/19), 280/282 (15/5), 227 (100), 195 (63), 91 (98); high resolution EIMS m/z 638.2764 (calcd for C 35 H 43 ClN 2 O 7 , -0.5 mmu error), 412.1516 (calcd for C 20 H 27 C1NO 6 , 1.1 mmu error), 227.1293 (calcd for C 15 H„NO, 1.0 mmu error). Η NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.77 (2, d; 15.5), 6.68 (3, ddd; 15.5, 9.5 and 5.3), 2.37 (4, m), 2.54 (4, m), 5.01 (5, ddd; 11.4, 6 and 1.5), 2.56 (6, m), 1.14 (6-Me, d; 7.0;, 6.01 (7, dd; 15.8 and 8.8), 6.41 (8, d; 15.8), 7.28-7.34 (10/11/13/14, m), 7.23 (12, m); leucic acid (D) 4.84 (2, dd; 10.1 and 3.6), 1.62 (3, m), 1.3 (3, m), 1.62 (4, m), 0.77 (5, d; 6.5), 0.73 (5' , d; 6.3); 3-amino-2-methylpropionic acid (C) 2.7 (2, m), 1.22 (2-Me, d; 7.3), 3.28 (3, dt; 13.5 and 7.0), 3.50 (3, ddd; 13.5, 4.9 and 4), 6.93 (3 NH, brt; 6.3); 3-chloro-4-methoxyphenylalanine (B) 4.82 (2, m), 5.64 (2-NH, d; 8.8), 3.05 (3, dd; 14.5 and 7.0), 3.13 (3, dd; 14.5 and 5.5), 7.22 (5, d; 2.2), 3.87 (7-OCH 3 , s), 6.84 (8, d; 8.5), 7.08 (9, dd; 8.5 and 2.2). 13 C NMR (CDC1 3 ): unit δ (carbon position) 165.4 (1), 125. (2), 141.4 (3), 36.5 (4), 77.1 (5), 42.3 (6), 17.3 (6-Me), 130.1(7), 130.0 (8), 136.7 (9), 126.1 (10/14), 128.6 (11/13), 128.4 (12); D 170.1 (1), 71.6 (2), 39.5 (3), 24.5 (4), 21.2 (5), 22.7 (5'); C 175.6 (1), 38.3 (2), 14.0 (2-Me), 41.2 (3); B 170.9 (1), 53.5 (2), 35.1 (3), 129.8 (4), 131.0 (5), 122.4 (6), 154.0 (7), 56.1 (7-OCH 3 ), 112.2 (8), 127.6 (9). Cryptophycin 4 [α] D +36.7°(MeOH, c 1.93); UN) λ^e) 206 (41,800), 228 (25,000), 240 (21,200), 248 (22,500), 280 (3,000), 290 (1,230); CD [Θ] 20S +63,900, [β] 2I1 +3,040, [Θ] 2I8 -71,900, [θ] 229 - 11,700, [θ] 234 -130,[θ] 252 +47,500, [θ] 270 +5,400. IR (CHCI ^ 3410, 2962, 2917, 1741, 1718, 1678, 1511, 1251 cm- 1 . EIMS m/z (rel intensity) 604 (2, M+), 378 (74), 246 (11), 227 (46), 161 (100), 91 (96); high resolution EIMS m/z 604.3127 (calcd for C^H-^O,, 2.2 mmu error), 378.1910 (calcd for C 20 H 28 ΝO 6 , 0.7 mmu error), 227.1293 (calcd for C 15 H I7 NO, 1.7 mmu error), 161.0605 (calcd for C 10 H 9 O 2> -0.2 mmu error). Η NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.74 (2, dd; 15.3 and 1.2), 6.71 (3, ddd; 15.3, 10.3 and 5.0), 2.37 (4, m), 2.53 (4, m), 5.03 (5, ddd; 11.2, 6.4 and 2.0), 2.55 (6, m), 1.13 (6-Me, d; 6.8), 6.01 (7, dd; 15.8 and 8.8), 6.40 (8, d; 15.8), 7.28-7.37 (10/11/13/14, ), 7.22 (12, m); leucic acid (D) 4.84 (2, dd; 10.1 and 3.6), 1.65 (3, m), 1.34 (3, m), 1.65 (4, m), 0.75 (5, d; 6.5), 0.72 (5", d; 6.3); 3-amino-2- methylpropionic acid (C) 2.69 (2, m), 1.22 (2-Me, d; 7.5), 3.39 (3-H 2 , m), 7.03 (3-NH, brt; 6.0); O-methyltyrosine (B) 4.79 (2, m), 5.61 (2-NH, d; 7.8), 3.08 (3, dd; 14.5 and 7.0), 3.13 (3, dd; 14.5 and 5.3), 7.11 (5/9, d; 8.8), 6.81 (6/8, d; 8.8), 3.78 (7-OCH 3 , s). ,3 C NMR (CDC1 3 ): unit δ (carbon position) ,4 165.3 (1), 125.1 (2), 141.5 (3), 36.5 (4), 77.1 (5), 42.3 (6), 17.3 (6-Me), 130.1 (7), 131.8 (8), 136.7 (9), 126.2 (10/14), 128.7 (11/13), 127.6 (12); D 170.8 (1), 71.6 (2), 39.5 (3), 24.5 (4), 21.2 (5), 22.7 (5'); C 175.9 (1), 38.2 (2), 14.2 (2-Me), 40.9 (3); B 171.2 (1), 53.8 (2), 35.3 (3), 131.0 (4). 130.2 (5/9), 114.1 (6/8), 158.6 (7), 55.2 (7- OCH 3 ). Crvptophvcin 5 [α] D +36.0° (MeOH, c 0.55); UV λ^e) 206 (45,600), 218 (37,700), 280 (3,790), 286 (3,480), 325 (2,080); CD [θ] 203 +7,710, lθ] 206 +29,000, [θ] 210 +21,400, [θ] 222 +59,800, [θ] 234 + 12,800, [θ] 241 + 13,700. IR (CHC1 3 ) v ^ 3426, 2958, 1728, 1672, 1502, 1259 cm 1 . EIMS m/z (rel intensity) 686/688 (0.1510.05), 655/657 (1/0.3), 654/656 (1.5/0.5), 311/313 (75/27), 195 (66), 155 (54), 121 (51), 91 (100); high resolution EIMS m/z 686.2983 (calcd for C 36 H 47 CIN 2 0 9 , -1.3 mmu error). 'H NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in -Hz) 7,8-epoxy-5-hydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.87 (2, d; 15.3), 6.72 (3, dt; 15.3 and 6.8), 2.60 (4, m), 2.52 (4, ddd; 15.2, 7.8, and 6.8), 5.11 (5, ddd; 12.3, 7.8, and 7.1), 1.87 (6,m), 1. 12 (6-Me, d; 7.1), 2.91 (7, dd; 7.3 and 2.1), 3.70 (8, d; 2.1), 7.24 (10/14, brd; 7.4), 7.29-7.36 (11/12/13, m); leucic acid (D) 4.09 (2, m), 2.86 (2- OH, brd, 6.1), 1.83 (3, m), 1.42 (3, m), 1.86 (4, m), 0.90 (5, d; 6.6), 0.87 (5' , d; 6.8); 3- amino-2-methylpropionic acid (Q 3.64 (I -OCH 3 , s), 2.60 (2, m), 1.07 (2-Me, d; 7.3), 3.27 (3, ddd; 13.5, 8.0 and 5.5), 3.39 (3, m), 6.32 (3-NH, t; 5.4); 3-chloro-4-methoxyphenylalanine (B) 4.59 (2, dt; 6 and 7.5), 6.30 (2-NH, d; 7.5), 2.95 (3, dd; 13.6 and 7.5), 3.0 (3, dd; 13.6 and 6.0), 7.2 (5, d; 2. 1), 3.86 (7-OCH,, s), 6.84 (8, d; 8.5), 7.05 (9, dd, 8.5; 2.1). 13 C NMR (CDC1 3 ): unit δ (carbon position) A 164.8 (1). 126.5 (2), 139.2 (3), 34.4 (4), 75.5 (5), 39.2 (6), 12.9 (6-Me), 63.3 (7), 58.7 (8), 136.8 (9), 125.7 (10/14), 128.6 (11/13), 128.4 (12); D 175.1 (1), 69.2 (2), 43.2 (3), 24.3 (4), 21.2 ' (5), 23.2 (5'); C 175.4 (1), 51.9 (1-OMe), 39.1 (2), 14.7 (2-Me), 41.6 (3); D 170.6 (1), 54.6 (2), 37.4 (3), 129.5 (4), 131.0 (5), 122.4 (6), 154.1 (7), 56.1 (7-OMe), 112.2 (8), 128.4 (9). Cryptophycin 6 [α] D + 17.1 °)(MeOH, c 1.1); UVλ^e) 206 (40,000), 218 (30,100), 228 (21,400), 282 (2,430); CD [θ] 203 +37,700, [θ] 210 -5,430, [θ] 213 -1,260, [θ] 221 +24,100, [θ] 232 +8,480, [θ] 240 + 13,400, [θ] 254 +790. IR (CHC1 3 ) v max 3425, 3006, 2956, 1726, 1672, 1641, 1502, 1462, 1259 cm 1 . FABMS (thioglycerol) m/z, (rel intensity) 573/575 (13/6) [M-H 2 O] + , 217 (26), 91 (100). Η NMR(CDC1 3 ): amino or hydroxy acid unit 6 (carbon position, multiplicity; J in Hz) 5, 7,8- trihydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.92 (2, dt; 15.0 and 1.5), 6.94 (3, dt; 15 and 7.5), 2.51 (4, m), 2.64 (4, m), 3.97 (5, ddd; 9.3, 6.5 and 4.5), 2.03 (6, m), 1.10 (6-Me, d; 6.5), 3.70 (7, dd; 9.0 and 7.5), 4.64 (8, d; 7.5), 7.33-7.39 (10/11/13/14, m), 7.28 (12, tt; 6.5 and 2.0); 3-chloro-4-methoxyphenylalanine (B) 4.60 (2, td; 8.0 and 6.0), 6.09 (2-NH, brd; 8.0), 2.96 (3, dd; 13.8 and 8.0), 3.02 (3, dd; 13.8 and 6.0), 7.22 (5, d; 2.0), 3.86 (7-OCH 3 , s), 6.84 (8, d; 8.5), 7.07 (9, dd;. 8.5 and 2.0) 3-amino-2-methylpropionic acid (C) 3.63 (l-OCH 3 ,s), 2.5 (2, m), 1.07 (2-Me, d; 7.0), 3.24 (3, ddd; 13.8, 8 and 6.5), 3.41 (3, ddd; 13.8, 6.5 and 4.8), 6.21 (3-NH, brt; 6.5). 13 C NMR (CDC1 3 ): unit δ (carbon position) A 165.2 (1), 125.6 (2), 141.3 (3), 36.9 (4), 82.5 (5), 46.3 (6), 14.3 (6-Me), 85.1 (7), 84.8 (8), 140.9 (9), 125.8 (10/14), 128.6 (11/13), 127.8 (12); B 170.6 (1), 54.5 (2), 37.3 (3), 129.6 (4), 131.0 (5), 122.5 (6), 154.1 (7), 56.1 (7-OCH 3 ), 112.2 (8), 128.5 (9) C 52.0 (l-OCH 3 ), 175.4 (1), 39.2 (2), 14.7 (2- Me), 41.6 (3). Cryptophycin 7 [ ] D -51.9° (MeOH, c 0.89); UV λ^e) 206 (23,400), 220 (14,900), 282 (1,670); CD [θ] 202 +35,400, [θ] 206 -1,730, [Θ] 2U -19,200, [θ] 220 -15,800, [θ] 232 +29,000, [θ] 263 +2,040. IR (CHC1 3 ) v max 3426, 2946, 1732, 1675, 1501, 1258 cm 1 . EIMS m/z (rel intensity) 455/457 (1/0.3, [M-2H 2 O] + ), 105 (100), 77 (98); FABMS m/z (magic bullet matrix) 496/498 [M-H 2 O+Na] + , (thioglycerol matrix) 474/476 [M-H,Ol +H] + . Η NMR (CD 3 OHD): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 5, 7, 8-trihydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 6.06 (2, ddd; 15.5, 1.3 and 1.0), 6.80 (3, dt; 15.5 and 7.5), 2.49 (4, m), 2.59 (4, m), 3.92 (5, ddd; 9.5, 6.3 and 4.7), 1.95 (6, m), 1.08 (6-Me, d; 6.7), 3.59 (7, dd; 9.0 and 7.8), 4.56 (8, d; 7.8), 7.37 (10/14, brd; 7.3), 7.31 (11/13, brt; 7.3), 7.24 (12, tt; 7.3 and 1.5); 3-chloro-4- methoxyphenylalanine (B) 4.52 (2, dd; 6.9 and 5.0), 2.93 (3, dd; 13.8 and 6.9), 3.15 (3, dd; 13.8 and 5.0), 7.20 (5, d; 2.2), 3.78 (7-OCH 3 , s), 6.88 (8, d; 8.4), 7.08 (9, dd; 8.4 and 2.2). ,3 C NMR (CD 3 OD): unit δ (carbon position) A 167.4 (1), 127.6 (2), 140.9 (3), 37.9 (4), 84.0 (5), 47.6 (6), 14.4 (6-Me), 86.0 (7), 85.8 (8), 142.9 (9), 127.1 (10/14), 129.3 (11/13), 128.5 (12); B 177.6 (1), 57.3 (2), 38.2 (3), 132.8 (4), 132.1 (5), 122.9 (6), 155.0 (7), 56.5 (7-OCH 3 ), 113.2 (8), 130.1 (9). Cryptophycin 16 [α] D + 41.3° (MeOH, c 5.2); UV ._ (€) 242 (4963), 280 (2430), 286 (2212); IR (neat) v max 3402, 3270, 2960, 1748, 1724, 1676, 1514, 1466, 1343, 1239, 1177 cm 1 ; EIMS m/z (rel intensity) 640/642 (66/27), 398/400 (47/16), 265 (55), 227 (93), 181 (100); high resolution EIMS m/z 640.25676 (calcd for C^H-.CIN , -1.6 mmu error). 'H NMR (CDC1 3 ): amino or hydroxyacid unit δ (carbon position, multiplicity; J in Hz) 7, 8-epoxy-5-hydroxy—6- methyl-8-phenyl-2-octenoic acid (A) 5.74 (2, d; 16), 6.67 (3, ddd; 15.3, 9.7 and 5.5), 2.45 (4, dt; 14.3 and 10.4), 2.55 (4, brdd; 14.3 and 5.3), 5.15 (5, ddd; 11.2, 4.8 and 1.8), 1.8 (6, m), 1.14 (6-Me, d; 7.0), 2.92 (7, dd; 7.5 and 2.0), 3.69 (8, d; 2.0), 7.24-7.26 (10/14, m), 7.33-7.39 (11/12/13, m); 3-chloro-4-hydroxyphenylalanine (B) 4.8 (2, m), 5.64 (2-NH, d; 8.8), 3.03 (3, dd; 14.5 and 7.0), 3.11 (3, dd; 14.4 and 5.6), 7.17 (5, d; 2.2), 5.61(7-O s), 6.91 (8, d; 8.3), 7.0 (9, dd; 8.3 and 2.2); 3-amino-2-methylpropionic acid (Q 2.71 (2, m), 1.22 (2-Me, d; 7.3), 3.28 (3, dt; 13.6 and 6.8), 3.49 (3, ddd; 13.6, 5 and 4.1), 6.92 (3 NH, br t; 6.1); leucic acid (D) 4.83 (2, dd; 10.1 and 3.3), 1.36 (3, m), 1.67-1.75 (3, m), 1.67-1.75 (4, m), 0.85 (5, d; 7.5), 0.86 (5\ d; 6.8). 13 C NMR (CDCL) unit δ (carbon position) A 165.3 (1), 125.3 (2), 141.0 (3), 36.7 (4). 76.2 (5), 40.6 (6), 13.5 (6-Me), 63.0 (7), 59.0 (8), 136.8 (9), 125.6 (10/14), 128.7 (11/13), 128.6 (12); B 170.9 (1), 53.6 (2), 35.1 (3), 129.9 (4), 129.6 (5), 120.0 (6), 150.4 (7), 116.4 (8), 129.2 (9); C 175.6 (1), 38.3 (2), 14.1 (2-Me), 41.1 (3); D 170.8 (1), 71.3 (2), 39.4 (3), 24.6 (4), 21.3 (5), 22.9 (5'). Cryptophycin 17 [α] D + 27.8° (CHCLc. 0.37); UV λ ^ (e) 248 (14740), 268 (8100), 278 (3400), 284 (2840); IR (neat) v ^ 3412, 2958, 1750, 1723, 1668, 1504, 1463, 1290, 1177, 751 cm 1 ; EIMS m/z (rel intensity) 624/626 (10/3), 398/400 (95/35), 284 (100), 149 (95); high resolution EIMS m/z 624.26161 (calcd for CJ^.CINA, -1.4 mmu error). Η NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8- phenyl-2, 7-octadienoic acid (A) 5.77 (2, d; 15.4), 6.67 (3, ddd; 15.4, 9.5, and 5.3), 2.37 (4, m ), 4.99 (5, ddd; 11.2, 6.3, and 1.6), 2.54 (6, m), 1.14 (6-Me, d; 6.7), 6.01 (7, dd; 15.7, and 8.7), 6.41 (8, d; 15.9), 7.28-7.34 (10/11/13/14. m), 7.23 (12, m); 3-chloro-4- hydroxyphenylalanine (B) 4.82 (2, m), 5.63 (2-NH, d; 8.7), 3.12 (3, dd; 14.7, and 5.6), 3.03 (3', dd; 14.7, and 7.1), 7.18 (5, d; 2.0), 5.47 (7-OH, br s), 6.91 (8, d; 8.3), 7.02 ( 9, dd; 8.3, and 2.0); 3-amino-2-methylpropionic acid (Q 2.71 (2, m), 1.21 (2-Me, d' 6.9), 3.25 (3, m), 3.52 (3' , m), 6.89 (3-NH, br t; 6.1); luecic acid (D) 4.84 (2, dd; 9.6, and 3.1), 1.62 (3, m), 1.36 (3' , m), 1.62 (4, m), 0.77 (5. d 1 6.5), 0.73 (5' , d; 6.5); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.4 (1), 125.3 (2), 141.3 (3), 36.5 (4), 77.1 (5), 42.3 (6), 17.3 (6-Me), 130.0 (7), 129.9 (8), 136.7 (9), 126.2 (10/14), 128.6 (11/13), 127.6 (12); B 170.9 (1), 53.5 (2), 35.1 (3), 129.6 (4), 131.9 (5), 126.2 (6), 150.3 (7), 116.3 (8), 127.6 (9); C 175.9 (1), 38.4 (2), 13.9 (2-Me), 41.3 (3); D 170.9 (1), 71.6 (2), 39.5 (3), 24.5 (4), 21.2 (5), 22.7 (5'). Crvptophvcin 18 [α] D + 54.9° (MeOH, c 0.93); UV λ m (έ) 250 (20518), 284 (3857); IR (neat) v ^ 3411, 3271, 2966, 1746, 1728, 1668, 1505, 1463, 1258, 1178 cm 1 ; EIMS m/z (rel intensity 638/640 (4.5/1.1), 412/414 (59/19), 280(17), 227 (100); high resolution EIMS m/z 638.272934 (calcd for C 35 H 43 ClN 2 O 7 , 2.9 mmu error). 'H NMR (CDC1 3 ): amino or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8-phenyl-2, 7- octadienoic acid (A) 5.76 (2, d; 15.5), 6.65 (3, ddd; 15.4, 9.2 and 6.2), 2.38-2.47 (4, m), 5.08 (5,ddd; 10.6, 4.9 and 2.2), 2.58 (6, m), 1.15 (6-Me, d; 6.8), 6.07 (7, dd; 15.9 and 8.5), 6.43 (8, d; 15.9), 7.21-7.35 (10/11/12/13/14, m); 3-chloro-4-methoxy-phenylalanine ( 4.83 (2, m), 3.05(3, dd; 14.5 and 7.1). 5.65 (2-NH, d; 8.7), 3.14 (3, dd; 14.4 and 5.5), 7.21 (5, d; 2.4), 3.86 (7-OCH 3 , s), 6.83 (8, d; 8.3), 7.08 (9, dd; 8.3 and 2.2); 3-amino-2- methylpropionic acid (Q 2.73 (2, m), 1.23 (2-Me, d; 7.2), 3.23 (3, dt; 13.5 and 6.8), 3.56 (3, ddd; 13.5, 5.7 and 4.0), 6.85 (3-NH, dd; 7.1 and 6.2); leucic acid (D) 4.8 (2, d; 4.6), 1.86-1.89 (3, m), 0.94 (3-Me, d; 7.0), 1.20-1.26 (4, m), 1.39-1.44 (4, m), 0.77 (5, d; 7.4). 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.5 (1), 125.2 (2), 141.5 (3), 36.4 (4), 77.7 (5), 41.9 (6), 17.1 (6-Me), 129.8 (7), 131.9 (8), 136.8 (9), 128.6 (10/14), 126.2 (11/13), 127.6 (12); B 170.0 (1), 53.5 (2), 35.1 (3), 129.9 (4), 131.1 (5), 122.4 (6), 153.9 (7), 56.1 (7-OCH3), 112.2 (8), 128.5 (9); C 175.3 (1), 38.6 (2), 14.0 (2-Me), 41.4 (3); D 169.5 (1), 76.6 (2), 36.2 (3), 15.5 (3-Me), 24.2 (4), 14.0 (5). Cryptophycin 19 [α] D +62.6° (MeOH, c 0.67); UV (MeOH) λ maι (e) 204 (44900), 230 (17000), 248 (15600), 280 (2500); IR (neat) Vma , 3413, 3272, 2966, 1745, 1726, 1672, 1504, 1258, 1199, 1178, 1066, 692 cm 1 ; EIMS m/z (rel intensity) 624/626 (3.0/1.4), 398/400 (58/21), 280/282(15/5), 227 (100), 195/197 (57/22); high resolution EIMS m/z 624.2585 (calcd for C^H^CINA, 1.8 mmu error). 'H-NMR (CDCl 3 ):α/wmσ or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.76 (2, d; 15.2), 6.64 (3, ddd; 15.4, 9.1 and 6.2), 2.38 (4, m), 2.47 (4, m), 5.04 (5, ddd;7.1, 5.1 and 1.8), 2.57 (6, m), 1.15 (6-Me, d; 6.9), 6.05 (7, dd; 15.8 and 8.5), 6.43 (8, d; 15.8), 7.29-7.35 (10/11/13/14, m), 7.23 (12, m); 3-chloro-4-methoxyphenylalanine (B) 4.84 (2, m), 5.67 (2-NH, d; 8.9), 3.04(3, dd; 14.3 and 7.1), 3.14 (3, dd: 14.3 and 5.3), 7.22 (5, d; 2.0), 3.86 (7-OCH 3 , s), 6.83 (8, d; 8.2), 7.08 (9, dd; 8.2 and 2.0); 3-amino-2-methylpropionic acid (Q 2.75 (2, m), 1.23 (2-Me, d; 7.1), 3.19 (3, m). 3.59 (3, m), 6.80 (3-NH, brt; 6.7); 2-hydroxyisovaleήc acid (D) 4.73 (2, d; 4.2), 2.09 (3, m), 0.84 (4, d; 6.9), 0.95 (4' , d; 6.9). 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.5 (1), 125.3 (2), 141.3 (3), 36.3 (4) 77.7 (5), 42.0 (6), 17.1 (6-Me), 129.9 (7), 131.9 (8), 136.8 (9), 126.1 (10/14), 128.6 (11/13), 127.6 (12); B 171.0 (1), 53.4 (2), 35.1 (3), 130.0 (4), 131.1 (5), 122.4 (6), 153.9 (7), 56.1 (7-OMe), 112.2 (8), 128.5 (9); C 175.1 (1), 38.7 (2), 13.9 (2-Me), 41.5 (3), D 169.6 (1), 76.9 (2), 29.8 (3), 19.0 (4), 16.7 (3-Me). Cryptophycin 21 [α] D + 40.2° (CHC1 3 c 0.72); UV λmax (e) 240 (6700), 280 (2400), 288 (2100); IR (neat) v ^ 3403, 3279, 2957, 1731, 1673, 1503, 1464, 1409, 1372, 1258, 1174, 1065, 1023, 889 cm 1 ; EIMS m/z (relative intensity) 640/642 (10/4), 612 (5), 478 (15), 398 (40), 266 (33), 227 (76), 195 (95), 155 (100), 127 (90); high resolution EIMS m/z 640.2550 (calcd for C^H^CINA, 0.2 mmu error); 'H NMR (CDC1 3 ) amino or hydroxy acid unit δ (carbon positions, multiplicities; J in Hz) 7,8-epoxy-5-hydroxy-6-methyl-8-phenyl octanoic acid (A ) 5.73 (2, d; 15.4), 6.68 (3, ddd; 15.0, 9.9 and 4.9^, 2.45 (4, m), 2.56 (4, m), 5.19 (5, ddd; 11.2, 5.1 and 1.5), 1.80 (6, m), 1.14 (6-Me, d; 7.1), 2.92 (7, dd; 7.5 and 2.0), 3.68 (8, d; 1.8), 7.25 (10/14, m), 7.33-7.38 (11/12/13, m); 3- chloro-4-methoxyphenylalanine (.8 )4.74 (2, ddd; 8.2, 6.8 and 6.2), 5.68 (2-NH, d; 8.6), 2.98 (3, dd; 14.3 and 7.7), 3.14 (3, dd; 14.3 and 5.6), 7.21 (5, d; 2.0), 3.86 (7-OMe, s), 6.83 (8, d; 8.4), 7.07 (9, dd; 8.4 and 2.0); 3-aminopropionic acid (C )2.56 (2, m), 3.51 (3, m), 3.45 (3, m), 6.90 (3-NH, br t; 5.8); leucic acid (D )4.89 (2, dd; 10.0 and 3.3), 1.67 (3, m), 1.31 (3, m), 1.67 (4, m), 0.84 (5, d 6.4), 0.83 (5' , d; 6.4); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.5 (1), 125.3 (2), 141.0 (3), 36.7 (4), 75.9 (5), 40.6 (6), 13.5 (6-Me), 63.0 (7), 59.0 (8), 136.7 (9), 125.6 (10/14), 128.7 (11/13), 128.5 (12); B 170.7 (1), 53.9 (2), 35.0 (3), 129.8 (4), 130.9 (5), 122.4 (6), 153.9 (7), 56.1 (7-OMe), 112.2 (8), 128.3 (9); C 172.6 (1), 32.4 (2), 34.4 (3), D 170.5 (1), 71.2 (2), 39.5 (3), 24.4 (4), 22.8 (5), 21.2 (5'). Cryptophycin 23 [α] D + 47° (MeOH, c 1.55); UV λ rø (e) 240 (4571), 282 (2174), 290 (2177); IR (neat) v max 3284, 2960, 1747, 1724, 1653, 1540, 1490, 1339, 1272, 1174 cm 1 ; EIMS m/z (rel intensity) 674/675/678 (47/35/8), 432/434/436 (11/5/2), 299/301/303 (39/30/7), 227 (64), 215/217/219 (31/20/8), M l (100); high resolution EIMS m/z 674.21643 (calcd. for C j .H.CL.NA, -0-3 mmu error); Η NMR (CDC1 3 ) amino or hydroxyacid unit δ (carbon position, multiplicity; J in Hz) 7, 8-epoxy-5-hydroxy—6-methyl-8-phenyl-2-octenoic acid (A) 5.77 (2, d; 15.4), 6.65 (3, ddd; 15.4, 9.3 and 6.0), 2.47 (4, dt; 14.2 and 10.2), 2.55 (4, b dd; 14.2 and 5.6), 5.13 (5, ddd; 11.0, 4.6 and 1.6), 1.81 (6, m), 1.15 (6-Me, d; 6.9), 2.93 (7, dd; 7.6 and 2.0), 3.7 (8, d; 2.0), 7.22-7.26 (10/14, m), 7.32-7.39 (11/12/13, m); 3,5- dichloro-4-hydroxyphenylalanine (B) 4.81 (2, m), 5.69 (2-NH, d; 8.6), 3.11 (3, dd; 14.5 a 5.6), 3.50 (3, dd; 14.3 and 7.0), 7.13 (5/9, s), 5.78 (7-OH, s); 3-amino-2-methylpropionic acid (O 2.73 (2, m), 1.22 (2-Me, d; 7.1), 3.19 (3, dt; 13.4 and 6.9), 3.58 (3, ddd; 13.6, 5 and 4.1), 6.82 (3-NH, br t; 5.9); leucic acid (D) 4.84 (2, dd; 9.9 and 3.2), 1.38 (3, m), 1.68-1.75 (3, m), 1.68-1.75 (4, m), 0.86 (4-Me, d; 6.7), 0.87 (5, d; 6.7). 13 C NMR (CDC1 3 unit δ (carbon position) A 165.4 (1), 125.4 (2), 140.9 (3), 36.7 (4), 76.3 (5), 40.6 (6), 13.5 (6-Me), 63.0 (7), 58.9 (8), 136.7 (9), 125.6 (10/14), 128.7 (11/13), 128.6 (12); B 170.7 (1 53.3 (2), 35.0 (3), 130.3 (4), 129.0 (5/9), 121.0 (6/8), 146.7 (7); C 175.3 (1), 38.4 (2), 13 (2-Me), 41.5 (3); D 170.8 (1), 71.3 (2), 39.4 (3), 24.6 (4), 21.3 (4-Me), 22.9 (5). Cryptophycin 24 [α] D + 48.8° (CHC1 3 , c 0.63); UV λ„ (e) 228 (19006), 242 (8249), 274 (2351); IR (neat) v mx 3400, 3284, 2959, 1732, 1678, 1652, 1514, 1248, 1178 cm 1 ; EIMS m/z (rel intensity, assignment) 606 (2, M + ), 364 (7), 161 (55, CH 3 O-C 6 H 4 -CH=CH=CO + ), 121 (100 CH 3 O-C 6 H 4 -CH 2 + ), 91 (68); high resolution EIMS m/z 606.2954 (calcd for C^NA, -1.3 mmu error); Η NMR (CDC1 3 ) amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 7,8-epoxy-5-hydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.70 (2, dd; 15.2 and 1.3) 6.70 (3, ddd; 15.2, 10.3 and 4.7), 2.43 (4, dt; 14.3 and 10.9), 2.56 (4, m), 5.20 (5, ddd; 11.3, 5.1 and 2.0), 1.79 (6, m), 1.14 (6-Me, d; 7.0), 2.92 (7, dd; 7.5 and 2.0), 3.68 (8, d; 2.0), 7.23 - 7.38 (10/11/12/13/14, m); 0-methyltyrosine (B) 4.73 (2, m), 5.58 (2-NH, d; 8.3), 3.03 (3, dd; 14.5 and 7.5), 3.14 (3, dd; 14.5 and 5.7), 7.11 (5/9, d; 8.6), 6.81 (6/8, d 8.6), 3.78 (7-OMe, s); 3-aminopropionic acid (C) 2.55 (2-H 2 , m), 3.42 (3, m), 3.53(3, m), 6.97 (3-NH, br t; 5.7); leucic acid (D) 4.89 (2, dd; 9.9 and 3.5), 1.29 (3, m), 1.62 - 1.70 (3/4, m), 0.83 (5, d; 5.9), 0.84 (5' , d; 6.1); "C NMR (CDC1 3 ): unit δ (carbon position) A 165.4 (1), 125.3 (2), 141.0 (3), 36.7 (4), 75.9 (5), 40.6 (6), 13.4 (6-Me), 63.0 (7), 59.0 (8) 136.7 (9), 125.6 (10/14), 128.7 (11/13), 12S.5 (12); B 170.7 or 170.6 (1), 54.1 (2), 35.2 (3), 128.5 (4), 130.2 (5/9), 114.1 (6/8), 158.6 (7). 55.2 (7-OMe); C 172.8 (1), 32.5 (2), 34.2 (3); D 170.6 or 170.7 (1), 71.2 (2), 39.5 (3), 24.4 (4). 21.3 (5), 22.8 (5 1 ). Crvptophvcin 26 [o] D + 28.2° (CHCl,, c 1.31); UV λ m (έ) 254 (14615), 284 (2949); IR (neat) Vmax 3299, 2960, 1732, 1644, 1504, 1258, 1209 cm 1 ; EIMS m/z (rel intensity) 656/658 (0.5/0.1, M + ), 638/640 (1.7/1.0), 525/527 (3.7/1.8), 412/414 (10/4), 280/282 (12/11), 227 (20), 195 (48), 131 (68); high resolution EIMS m/z 656.2836 (calcd for C 35 H 45 ClN 2 O 8 , 2.8 mmu error), 638.2712 (calcd for C 35 H 43 ClN 2 O 7 , 4.7 mmu error); Η NMR (CDC1 3 ) amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 3,5-dihydroxy-6-methyl-8-phenyl-7-octenoic aci (A) 2.46 (2, dd; 14.8 and 7.8), 2.58 (2, dd; 14.8 and 3.0), 5.46 (3, m), 1.86 - 1.90 (4-H 2 , m), 3.61 (5, m), 2.37 (6, m), 1.14 (6-Me, d; 6.8). 6.06 (7, dd; 16 and 8.7), 6.47 (8, d; 16), 7.37 (10/14, br d; 7.9), 7.32 (11/13, br t; 7.6), 7.22 - 7.28 (12, m); 3-chloro-4- methoxyphenylalanine (B) 4.73 (2, br dt; 6.4 and 8.1), 6.14 (2-NH, d; 8.6), 2.84 (3, dd; 14.4 and 8), 3.18 (3, dd; 14.4 and 6.3), 7.21 (5, d; 2.2), 3.85 (7-OMe, s), 6.82 (8, d; 8.6), 7.08 (9, dd; 8.6 and 2.2); 3-amino-2-methylpropionic acid (C) 2.87 (2, m), 1.19 (2-Me, d; 7.0), 3.01 (3, ddd; 13.4, 10.6 and 4.9), 3.73 (3, ddd; 13.4, 8.2 and 4.7), 6.72 (3-NH, br dd; 7.3 and 5.2); leucic acid (D) 4.95 (2, dd; 9.7 and 4.2), 1.62 - 1.72 (3, m), 1.79 - 1.84 (3, m), 1.62 - 1.72 (4, m), 0.90 (4-Me, d; 6.4), 0.95 (5, d; 6.4). 13 C NMR (CDC1 3 ): unit δ carbon position) A 170.0 (1), 41.5 (2), 71.4 (3), 37.3 (4), 71.9 or 71.8 (5), 43.6 (6), 16.6 (6-Me), 130.8 (7), 132.5 (8), 136.8 (9), 126.2 (10/14), 128.6 (11/13), 127.6 (12); B 170.9 (1), 53.2 (2), 34.7 (3), 130.3 (4), 131.1 (5), 122.2 (6), 153.8 (7), 56.1 (7-OMe), 112.2 (8), 128.5 (9); C 174.3 (1), 40.1 (2), 14.4 (2-Me), 42.5 (3); D 170.7 (1), 71.8 or 71.9 (2), 38.9 (3), 24.6 (4), 21.6 (4-Me), 22.9 (5). Crvptophcvin 28 [α] D + 65.6° (MeOH, c 0.93); UV (MeOH) ^ (e) 204 (48000), 230 (19300), 248 (18700), 280 (3400); IR (neat) v ^ 3413, 3270, 2958, 1745, 1726, 1665, 1504, 1258, 1197, 1175, 1066, 694 cm 1 ; EIMS m/z (rel intensity) 624/626 (3.0/1.3), 412/414 (70/24), 280/282(13/6), 213 (100), 195/197 (86/40); high resolution EIMS m/z 624.2626 (calcd for C^H u ONA, -2.4 mmu error); Η NMR(CDC1 3 ) amino or hydroxy acid unit δ ( carbon position, multiplicity; Jin Hz) 5-hydroxy-8-phenyl-2, 7-octadienoic acid (A) 5.78 (2, d; 15.6), 6.71(3, ddd; 15.6, 9.9 and 5.4), 2.40 (4, m), 2.53 (4, m), 5.17 (5, m), 2.53 (6-H 2 , br t; 6.7), 6.07 (7, dt; 15.8 and 7.4), 6.44 (8, d; 15.8), 7.27-7.38 (10/11/13/14, m), 7.22 (12, m); 3-chloro-4-methoxyphenylalanine (B) 4.82 (2, m), 5.72 (2-NH, d; 8.5), 3.04 ( 3, dd; 14.5 and 7.2), 3.14 (3, dd; 14.5 and 5.4), 7.22 (5, d; 2.0), 3.87 (7-OMe, s), 6.84 (8, d; 8.5), 7.08 (9, dd; 8.5 and 2.0); 3-amino-2-methylpropionic acid (Q 2.72 (2, m), 1.21 (2-Me, d; 7.2), 3.29 (3, dt; 13.5 and 7.0), 3.49 (3, ddd; 13.5, 4.9 and 3.8), 6.97 (3-NH, br t; 5.6); leucic acid (D) 4.82 (2, m), 1.40 (3, m), 1.62 (3, m), 1.62 (4, m). 0.76 (4-Me, d; 6.3), 0.74 (5, d; 6.3); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.4 (1), 125.2 (2), 141.2 (3), 38.5 (4), 73.5 (5), 38.6 (6), 124.1 (7), 133.8 (8), 136.7 (9), 126.1 (10/14), 128.6 (11/13), 127.6 (12); B 170.9 (1), 53.6 (2), 35.1 (3), 129.8 (4), 131.0 (5), 122.4 (6), 154.0 (7), 56.1 (7-OMe), 112.3 (8), 128.4 (9); C 175.6 (1), 38.3 (2), 14.0 (2-Me), 41.2 (3), D 170.9 (1), 71.6 (2), 39.6 (3), 24.5 (4), 21.5 (4-Me), 22.6 (5). Cryptophycin 29 [α] D + 22.2° (CHC1 3 , c 1.13); UV λ πm ( e ) 250 (17000), 284 (3300); IR (neat) v TO 3415, 3272, 2960, 1744, 1734, 1674, 1504, 1259, 1197, 1174, 1067, 694 cm 1 ; EIMS m/z (rel intensity) 624/626 (2.6/1.1), 398/400 (44/15), 227 (100), 195/197 (50/16), 155/157 (59/20), 131 (63), 91 (95); high resolution EIMS m/z 624.2607(calcd for C^.CINA, -0.5 mmu error); Η NMR(CDC1 3 ) amino or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.75 (2, dd; 15.3 and 1.1), 6.6 (3, ddd; 15.3, 10.1 and 5.3), 2.36 (4, m), 2.54 (4, m), 5.03 (5, ddd; 11.0, 6.4 and 1.8), 2.56 (6, m), 1.14 (6-Me, d; 6.8), 6.01 (7, dd; 15.8 and 8.8), 6.41 (8, d; 15.8), 7.28-7.33 (10/11/13/14, m), 7.22 (12, m); 3-chloro-4-methoxyphenylalanine (B) 4.76 (2, m), 5.67 (2- NH, d; 8.6), 3.0 ( 3, dd; 14.4 and 10.2), 3.14 (3, dd; 14.4 and 5.9), 7.22 (5, d; 2.2), 3.87 (7-OMe, s), 6.83 (8, d; 8.4), 7.08 (9, dd; 8.4 and 2.2); 3-aminopropionic acid (Q 2.55 (2- H 2 , m), 3.44 (3, m), 3.55 (3, m), 6.89 (3-NH, br t; 5.7); leucic acid (£>) 4.90 (2, dd; 9.9 and 3.5), 1.34 (3, ddd; 15.4, 10.3 and 3.5), 1.63 (3, m), 1.63 (4, m). 0.76 (4-Me, d; 6.4), 0.72 (5, d; 6.4); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.6 (1), 125.2 (2), 141.5 (3), 36.4 (4), 77.1 (5), 42.3 (6), 17.3 (6-Me), 130.1 (7), 131.8 (8), 136.7 (9), 126.2 (10/14), 128.6 (11/13), 127.6 (12); B 170.9 (1), 53.8 (2), 34.9 (3), 129.9 (4), 131.0 (5), 122.4 (6), 153.9 (7), 56.1 (7-OMe), 112.2 (8), 128.4 (9); C 172.6 (1), 32.4 (2), 34.5 (3); D 170.4 (1), 71.5 (2), 39.7 (3), 24.4 (4), 21.2 (4-Me), 22.6 (5). Cryptophycin 30 [α] D - 12.3° (CHC1 3 , c 1.53); UV λ n > (e) 254 (17200), 284 (3600); IR (neat) v max 3414, 3306, 2961 , 1738, 1729, 1660, 1504, 1258, 1205, 1183, 1066, 695 cm 1 ; EIMS m/z (rel intensity) 656/658 (1.0/0.3), 638/640 (3.0/1.0), 525/527 (3.8/1.3), 412/414 (10.5/3.6), 280/282(10.3/3.8), 227 (29), 195/197 (48/17), 155/157 (74/21), 131 (100); high resolution EIMS m/z 656.2852(calcd for C 35 H 45 ClN 2 O 8 , 1.3 mmu error); Η-NMR(CDCl 3 ):αm σ or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 3,5- dihydroxy-6-methyl-8-pheny 7-octenoic acid (A) 2.25 (2, dd; 16.0 and 9.6), 2.64 (2, brd; 16.0), 3.89 (3, m), 2.51 (3-O d; 6.4), 1.77 (4, ddd; 14.3, 9.8 and 2.1), 1.88 (4, ddd; 14.3, 11.3 and 3.8), 4.88 (5, ddd; 11.3, 6.2 and 2.1), 2.53 (6, m), 1.10 (6-Me, d; 6.8), 5.99 (7, dd; 15.9 and 9.0), 6.40 (8, d 15.9), 7.28-7.33 (10/11/13/14, m), 7.23 (12, m); 3-chloro-4-methoxyphenylalanine (B) 4.60 (2, m), 6.61 (2-NH, d; 8.1), 3.09 ( 3, dd; 14.2 and 5.6), 3.15 (3, dd; 14.2 and 7.3), 7.22 (5, d; 2.1), 3.86 (7-OMe, s), 6.83 (8, d; 8.3), 7.07 (9, dd; 8.3 and 2.1); 3-amino-2- methylpropionic acid (Q 2.67 (2, m), 1.21 (2-Me, d; 7.3), 3.26 (3, ddd; 13.6, 7.3 and 6.4), 3.63 (3, ddd; 13.6, 6.2 and 3.9), 6.75 (3-NH, br t; 6.3); leucic acid (D) 4.83 (2, dd; 9.6, 4.1), 1.42 (3, m), 1.64 (3, m), 1.64 (4, m). 0.79 (4-Me, d; 6.4), 0.76 (5, d; 6.4); ,3 C NMR (CDC1 3 ) unit δ (carbon position) A 171.6 (1), 42.4 (2), 66.0 (3), 41.3 (4), 76.0 (5), 42.0 (6), 17.3 (6-Me), 130.0 (7), 131.9 (8), 136.7 (9), 126.1 (10/14), 128.6 (11/13), 127.6 (12); B 170.8 (1), 54.3 (2), 35.1 (3), 130.1 (4), 131.1 (5), 122.2 (6), 153.8 (7), 56.1 (7-OMe), 112.1 (8), 128.7 (9); C 175.6 (1), 39.7 (2), 13.8 (2-Me), 41.5 (3), D 171.9 (1), 72.1 (2), 39.1 (3), 24.6 (4), 21.4 (4-Me), 22.7 (5). Cryptophycin 31 [α] D + 50.6° (MeOH, c 1.13); UV A mM (e) 242 (3800), 284 (700); IR (neat) v max 3412 3272, 2961, 1745, 1725, 1678, 1537, 1481, 1270, 1196, 1176, 1000, 698 cm 1 ; EIMS m/z (rel intensity) 688/690/692 (1.2/1.0/0.4), 446/448/450 (7.9/6.7/3.1), 314/316/318 (17/11/3), 91 (100); high resolution EIMS m/z 688.2336 (calcd for C 35 H 42 Cl 2 N 2 O 8 , -1.8 mmu error); 'H- NMR (COCl 3 )amino or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 7,8- epoxy-5-hydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.78 (2, d; 15.5), 6.66 (3, ddd; 15.5, 9.4 and 6.0), 2.47 (4, ddd; 14.1, 10.8 and 9.4), 2.56 (4, m), 5.14 (5, ddd; 10.8, 4.7 and 1.7), 1.82 (6, m), 1.15 (6-Me, d; 7.1), 2.93 (7, dd; 7.5 and 1.9), 3.70 (8, d; 1.9), 7.24-7.2 (10/14, m), 7.34-7.39 (11/12/13, m); 3,5-dichloro-4-methoxyphenylalanine (B) 4.83 (2, m), 5.68 (2-NH, d; 9.0), 3.0 ( 3, dd; 14.4 and 7.3), 3.14 (3, dd; 14.4 and 5.6), 7.16 (5/9, s), 3.87 (7-OMe, s); 3-amino-2-methylpropionic acid (Q 2.74 (2, m), 1.22 (2-Me, d; 7.1), 3.20 (3, m), 3.58 (3, ddd; 13.5, 5.6 and 4.1), 6.82 (3-NH, br t; 5.6); leucic acid (£>) 4.83 (2, m), 1.38 (3, m), 1.72 (3, m), 1.72 (4, m). 0.87 (4-Me, d; 6.8), 0.86 (5, d; 6.8); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.4 (1), 125.4 (2), 141.0 (3), 36.7 (4), 76.3 (5), 40.6 (6), 13.5 (6-Me), 63.0 (7), 58.9 (8), 136.7 (9), 125.6 (10/14), 128.7 (11/13), 128.6 (12); B 170.8 (1), 53.3 (2), 35.2 (3), 129.3 (4), 129.6 (5/9), 134.5 (6/8), 151.2 (7), 60.6 (7-OMe); C 175.3 (1), 38.3 (2), 13.9 (2-CH3), 41.5 (3), D 170.6 (1), 71.3 (2), 39.4 (3), 24.6 (4), 22.9 (4-Me), 21.3 (5). Cryptophycin 40 [α] D + 41.6° (CHC1 3 , c 0.31); UV λ raax (e) 242 (4974), 266 (3911), 274 (3666), 286 (2359), 328 (511); IR (neat) v. ^ 3415, 2959, 1748, 1723, 1667, 1505, 1463, 1289, 1176 cm '; EIMS m/z (rel intensity) 640/642 (5/2), 280/282 (7/3), 213 (13), 195/197 (51/17), 155 (29), 141 (32), 121 (28), 91 (100), 69 (47); high resolution EIMS m/z 640.2570 (calcd. for C^H M CINA, -1.8 mmu error); 'H NMR (CDC1 3 ) amino or hydroxy acid unit _δ_(carbon positions, multiplicities; J in Hz) 7,8-epoxy-5-hydroxy-8-phenyl-2-octenoic acid (A) 5.77 (2, d; 15.1), 6.72 (3, ddd; 15.1, 9.7 and 4.9), 2.42 (4, m), 2.58 (4, m), 5.33 (5, m), 1.89 (6, ddd; 12.9, 8.1 and 5.0), 2.13 (6, ddd; 12.9, 9.3 and 5.0), 2.98 (7, ddd; 6.7, 4.5 and 1.9), 3.64 (8, d; 1.9), 7.31-7.39 (10/11/13/14, m), 7.22 (12, m); 3-chloro-4-methoxyphenylalanine ■ (B) 4.83 (2, m), 5.64 (2-NH, d; 8.6), 3.03 (3, dd; 14.3 and 7.5), 3.14 (3, dd; 14.3 and 5.4), 7.21 (5, d; 2.0), 3.87 (7-OMe, s), 6.84 (8, d; 8.3), 7.08 (9, dd; 8.3 and 2.0); 3-amino-2- methylpropionic acid (Q 2.72 (2, m), 1.23 (2-Me, d; 7.3), 3.31 (3, dt; 13.8 and 6.9), 3.50 (3, ddd; 13.6, 5.7 and 3.9), 6.96 (3-NH, br t; 6.0); leucic acid (£>) 4.85 (2, dd; 6.7, 3.4), 1.42 (3, m), 1.72 (3, m), 1.72 (4, m), 0.86 (4-Me, d, 3.7), 0.87 (5, d, 3.7); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.3 (1), 125.2 (2), 140.9 (3), 39.0 (4), 72.0 (5), 37.3 (6), 59.0 (7), 58.7 (8), 140.9 (9), 125.6 (10/14), 128.7 (11/13), 128.5 (12); B 170.9 (1), 53.6 (2), 35.1(3), 129.8 (4), 131.0 (5), 122.5 (6), 157.0 (7), 56.1 (7-OMe), 112.3 (8), 128.4 (9); C 175.6 (1), 38.3 (2), 14.1 (2-Me), 41.1 (3); D 170.9 (1), 71.4 (2), 39.4 (3), 24.5 (4), 21.5 (4-Me), 22.8 (5). Cryptophycin 43 [α] D + 20° (CHC1 3 , c 0.2); UV λ max (e) 250 (20512), 282 (4083), 294 (1734); IR (neat) v max 3400, 3272, 2927, 1727, 1660, 1516, 1455, 1242, 1175 cm 1 ; EIMS m/z (rel intensity) 533 (24), 484 (3), 445 (14), 398 (9), 364 (29), 227 (59), 149 (67), 91 (100) ; high resolution EIMS m/z 590.3044 (calcd for C w H 41 N 2 O 7 , -5.2 mmu error); Η NMR (CDC1 3 ) amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8- phenyl-2, 7-octadienoic acid {A) 5.75 (2, d; 15.3), 6.69 (3, ddd; 15.3, 9.9 and 5.3), 2.37 (4, dt; 14.2 and 10.4), 2.52 (4, m), 5.01 (5, ddd; 11.2, 6.4 and 1.8), 2.55 (6, m), 1.13 (6-Me, d; 6.9), 6.01 (7, dd; 15.8 and 8.9), 6.41 (8, d; 15.8), 7.21-7.34 (10/11/12/13/14, m); 4- methoxyphenylalanine (B) 4.80 (2, m), 5.64 (2-NH, d; 8.4), 3.06(3, dd; 14.5 and 7.2), 3.13 (3, dd; 14.4 and 5.3), 7.06 (5/9, d; 8.4), 6.74 (6/8, d; 8.4); 3-amino-2-methylpropionic acid (O 2.69 (2, m), 1.22 (2-Me, d; 7.3), 3.33 (3, m), 3.44 (3, dt; 14.0 and 4.7), 7.0 (3-NH, m); leucic acid (D) 4.84 (2, dd; 10.0 and 3.6), 1.60-1.67 (3, m), 1.35 (3, m), 1.60-1.67 (4, m), 0.76 (5, d; 6.4), 0.73 (5', d; 6.7); ,3 C NMR (CDC1 3 ) unit δ (carbon position) A 125.2 (2), 141.5 (3), 36.5 (4), 77.5 (5), 42.3 (6), 17.3 (6-Me), 130.1 (7), 131.8 (8), 136.8 (9), 126.2 (10/14), 128.6 (11/13), 127.6 (12); B 53.8 (2), 35.3 (3), 129.8 (4), 130.5 (5/9), 115.6 (6/8), 154.6 (7); C 38.3 (2), 14.1 (2-Me), 41.0 (3); D 71.6 (2), 39.6 (3), 24.5 (4), 21.2 (5), 22.9 (5'). Due to the small sample size, carbonyl carbon signals could not be seen. Cryptophycin 45 [α] D + 72.0° (MeOH, c 0.122); UV _ (e) 250 (25500), 284 (5300); IR (neat) v ^ 3407, 3239, 2958, 1743, 1727, 1667, 1538, 1469, 1242, 1196, 1177, 694 cm 1 ; EIMS m/z (rel intensity) 658/660/662 (2.1/1.4/0.3), 483 (7.6) 432/434/436 (9.5/6.4/1.8), 300/302/304 (8.0/5.5/1.2), 227 (100) 91 (87); high resolution EIMS m/z 658.2207 (calcd for C^H^C^NA, 0.6 mmu error); 'H-NMR(CDCl 3 ):α/ zø or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.80 (2, d; 14.7), 6.66 (3, ddd; 14.7, 8.5 and 5.5), 2.38 (4, m), 2.53 (4, m), 4.97 (5, br dd; 10.4 and 6.2), 2.57 (6, m), 1.14 (6-Me, d; 6.7), 6.01 (7, dd; 15.9 and 8.7), 6.42 (8, d; 15.9), 7.28-7.34 (10/11/1314, m), 7.22 (12; m); 3,5-dichloro-4-hydroxyphenylalanine (B) 4.82 (2, m), 5.73 (2-NH, br d; 8.7), 3.02 (3, dd; 14.3 and 6.2), 3.10 (3, dd; 14.3 and 5.2), 7.14 (5/9, s), 5.79 (7-OH, s); 3-amino-2-methylpropionic acid (Q 2.73 (2, m), 1.21 (2-Me, d; 7.0), 3.17 (3, m), 3.60 (3, m), 6.81 (3-NH, br t; 6.7); leucic acid (£>) 4.84 (2, dd;10.0 and 3.2), 1.38 (3, ddd; 14.9, 10.2 and 3.2), 1.65 (3, m), 1.65 (4, m). 0.78 (4-Me, d; 6.5), 0.73 (5, d; 6.5); 13 C NMR (CDC1 3 ) unit δ (carbon position) A 165.5 (1), 125.4 (2), 141.2 (3), 36.4 (4), 77.6 (5), 42.3 (6), 17.3 (6-Me), 130.0 (7), 131.9 (8), 136.7 (9), 126.2 (10/14), 128.6 (11/13), 127.6 (12); B 171.0 (1), 53.2 (2), 35.0 (3), 130.4 (4), 129.1 (5/9), 121.0 (6/8), 146.7 (7); C 175.2 (1), 38.5 (2), 13.9 (2-Me), 41.6 (3), D 170.7 (1), 71.5 (2), 39.5 (3), 24.6 (4), 22.7 (4-Me), 21.2 (5). Crvptophvcin 49 [α] D +68.1 ° (MeOH, c 0.075); UV λ max (e) 246 (25500), 284 (5200); IR (neat) v m 3401, 3282, 2962, 1744, 1728, 1668, 1540, 1505, 1464, 1258, 1198, 1177, 1066, 694 cm 1; EIMS m/z (rel intensity) 624/626 (0.8/0.3), 398/400 (43/14), 227(78), 195/197 (58/26) 91 (100); high resolution EIMS m/z 624.2650 (calcd for C^CINA, -4.8 mmu error); Η- NMR(CDCl 3 ):αmt>zo or hydroxy acid unit δ ( carbon position, multiplicity; J in Hz) 5- hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.77 (2, d; 14.1), 6.67 (3, m), 2.38 (4, m), 2.50 (4, m), 5.01 (5, m), 2.56 (6, m), 1.13 (6-Me, d; 6.5), 6.03 (7, dd; 15.8 and 8.6), 6.42 (8, d; 15.8), 7.29-7.35 (10/11/13/14, m), 7.23 (12; m); 3-chloro-4- methoxyphenylalanine (B) 4.82 (2, m), 5.64 (2-NH, m), 3.06 ( 3, m), 3.13 (3, m), 7.22 (5, m), 3.87 (7-OMe, s), 6.83 (8, m), 7.08 (9, m); 3-amino-2-methylpropionic acid (Q 2.72 (2, m), 1.22 (2-Me, d; 6.7), 3.26 (3, m), 3.53 (3, m), 6.90 (3-NH, m); 2-hydroxyvaleric acid (£>) 4.81 (2, dd; 8.8 and 3.9), 1.63 (3, m), 1.68 (3, m), 1.33 (4-H 2 , m). 0.74 (5, t; 7.3). Cryptophycin 50 [α] D + 32.0° (CHC1 3 c. 0.44); UN λ raax (e) 242 (4933), 262 (3996, 274 (3719), 286 (2430), 332 (359); IR (neat) v. ^ 3412, 3274, 2958, 1752, 1724, 1676, 1648, 1503, 1465, 1258, 1177, 1066, 753; EIMS m/z (rel intensity) 640/642 (4/2), 398/400 (11/4), 280/282 (10/3), 227 (17), 195/197 (57/18), 157 (20), 141 (31), 91 (100); high resolution EIMS m/z 640.2531 (calcd. for C^CIΝA, 2.1 mmu error); Η ΝMR (CDC1 3 ) amino or hydroxy acid unit_ h (carbon positions, multiplicities; J in Hz) 7,8-epoxy-5-hydroxy-6-methyl-8-phenyl octanoic acid (A) 5.73 (2, d; 15.7), 6.67 (3, ddd; 15.7, 9.7 and 5.4), 2.45 (4, m), 2.55 (4, m), 5.13 (5, ddd; 11.2, 5.0 and 1.7), 1.78 (6, m), 1.15 (6-Me, d, 6.9), 2.91 (7, dd; 7.5 and 1.9), 3.68 (8, d; 1.7), 7.25 (10/14, m), 7.33-7.38 (11/12/13; m); 3-chloro-4- methoxyphenylalanine (B) 4.80 (2, ddd; 8.3, 7.1 and 5.4), 5.61 (2-ΝH, d; 8.3), 3.03 (3, dd; 14.4 and 7.3), 3.13 (3, dd; 14.4 and 5.6), 7.21 (5, d; 1.9), 3.87 (7-OMe, s), 6.83 (8, d; 8.4), 7.07 (9, dd; 8.4 and 2.2); 3-amino-2-methylpropionic acid (C) 2.71 (2, m), 1.22 (2-Me, d; 7.3), 3.29 (3, dt; 13.6 and 6.9), 3.49 (3, ddd; 13.6, 6.7 and 5.0), 6.92 (3-NH, br t; 6.7); 2-hydroxypenanoic acid (D) 4.75 (2, dd; 9.2 and 3.7), 1.55 (3, m), 1.65 (3, m), 1.33 (4-H 2 , m), 0.84 (5, t; 7.3); 13 C NMR (CDC1 3 ) unit δ values (carbon positions) A 165.3 (1), 125.3 (2), 141.0 (3), 36.9 (4), 76.3 (5), 40.8 (6), 13.6 (6-Me), 63.2 (7), 59.1 (8), 136.8 (9), 125.5 (10/14), 128.7 (11/13), 128.5 (12); B 170.9 (1), 53.6 (2), 35.1 (3), 129.8 (4), 131.0 (5), 122.5 (6), 154.0 (7), 56.1 (7-OMe), 112.3 (8), 128.5 (9); C 175.6 (1), 38.4 (2), 14.1 (2- Me), 41.2 (3); D 170.4 (1), 72.4 (2), 32.7 (3), 18.4 (4), 13.5 (5). Cryptophycin 54 EIMS m/z (relative intensity) 654/656 (17/10), 493 (5), 411/413 (12/4), 280 (16), 22 (25), 195/197 (45/25), 141 (30), 91 (100); high resolution EIMS m z 654.2686 (calcd for C 35 H 43 ClN 2 O 8 , 2.2 mmu error); Η NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 5-hydroxy-6-methyl-7-oxo-8-phenyl-2-octenoic acid (A) 5.73 (2, d; 15.4), 6.66 (3, ddd; 15.4, 9.7, 5.7), 2.46 (4, m), 2.53 (4, m), 5.16 (5, ddd; 11.0, 4. 1.7), 1.79 (6, m), 1.14 (6-Me, d; 6.8), 2.89 (7, dd; 7.4, 1.8), 3.69 (8, d; 1.9), 7.25 (10/14, m), 7.30-7.38 (11/12/13, m); (B) 4.81 (2, m), 5.63 (2-NH, d; 8.6), 3.03 (3, dd; 14.5, 7.3), 3.13 (3, dd; 14.5, 5.5), 7.21 (5, d; 2.2), 3.87 (7-OMe, s), 6.83 (8, d; 8.4), 7.07 (9, dd; 8.4 2.2); (Q 2.73 (2, m), 1.22 (2-Me, d; 7.3), 3.26 (3, ddd; 13.4, 6.8, 6.8), 3.51 (3, ddd; 13.4 6.8, 5.3), 6.88 (3-NH, br t; 6.8); (D) 4.73 (2, d; 4.2), 1.78-1.82 (3, m), 0.92 (3-Me, d; 6.8), 1.36-1.41 (4, m), 1.18-1.20 (4, m), 0.80 (5, i; 7.5); 13 C NMR (CDC1 3 ): unit δ (carbon position) A 165.3 (1), 125.4 (2), 141.0 (3), 36.6 (4), 76.3 (5), 40.6 (6), 13.2 (6-Me), 63.1 (7), 58.7 (8), 136.7 (9), 125.4 (10/14), 128.6 (11/13), 128.5 (12); B 170.9 (1), 53.5 (2), 35.0 (3), 129.8 (4), 131.0 (5), 125.2 (6), 153.9 (7), 56.1 (7-OMe), 112.2 (8), 128.4 (9); C 175.4 (1), 38.5 (2), 14.0 (2-Me), 41.3 (3); D 169.4 (1), 76.5 (2), 36.1 (3), 15.6 (3-Me), 24.0 (4), 11.2 (5). Example 6 Synthesis of Cryptophycin Derivatives Cryptophycin 8 To a solution of 3.8mg of Cryptophycin 1 in 1.5mL of 2: 1 1,2-dimethoxy ethane/ water was added 9μL IN HCl. The solution was allowed to stir at room temperature for 4 h, neutralized with potassium carbonate, and evaporated. The residue was partitioned between water and CH 2 C1 2 . The CH 2 Cl 2 -soluble material was purified by reversed-phase HPLC to obtain 3.3mg of pure Cryptophycin 8. EIMS m/z (relative intensity) 690/692/694 (0.8/0.5/0.2). High resolution EIMS m/z 690.2533 (calcd for C^C^NA, -5-8 mmu error). Η NMR (CDCL,): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz) 8-chloro-5, 7-dihydroxy-6-methyl-8-phenyl- 2-octenoic acid (A) 5.79 (2, d; 15.4), 6.69 (3, ddd; 15.4, 9.7 and 5.6), 2.68 (4, ddt; 14.0, 5.5 and 1.8), 2.38 (4,m), 5.11 (5, ddd; 10.8, 8.6 and 1.8), 2.51 (6, m), 1.05 (6-Me, d; 7.0), 4.01 (7, dd; 9.6 and 1.9), 4.65 (8, d; 9.6), 7.36-7.41 (10/11/12/13/14, m); leucic acid (D) 4.92 (2, dd; 10.1 and 3.5), 1.76 (3/4, m), 1.45 (3, m), 0.94 (5, d; 6.6), 0.94 (5', d; 6.4); amino-2-methylpropionic acid (C) 2.73(2, m), 1.22 (2-Me, d; 7.2), 3.25 (3, ddd; 13.6, 6.8 and 6.1), 3.54 (3, ddd; 13.5, 6.1 and 3.4), 6.91(3-NH, brt; 6. 1); 3-chloro-4- methoxyphenylalanine (B) 4.82 (2, ddd; 8.8, 7.2 and 5.6), 5.64 (2-NH, d; 8.8), 3.03 (3, dd; 15.4 and 7.2), 3.16 (3, dd; 15.4 and 5.6), 7.23 (5, d; 2.2), 3.88 (7-OCH 3 , s), 6.85 (8, d; 8.5), 7.09 (9, dd; 8.5 and 2.2). Cryptophycin 9 To a solution of 10 mg of Cryptophycin 1 in lmL dry methanol was added lOμL methanolic HCl (obtained by treating 1.25g thionyl chloride with 25mL MeOH). After stirring for 4 h the solvent was removed in vacuo and the sample was left under vacuum for 12 h. Reversed-phase HPLC gave 8mg of pure Cryptophycin 9. Η NMR (CDC1 3 ): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz); 5, 7-dihydroxy-8-methoxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.76 (2, d; 15.5), 6.67 (3, ddd; 15.5, 9.5 and 5.6), 2.34 (4, ddd; 14.1, 11.1 and 9.5), 2.62 (4, dddd; 14.1, 5.6, 1.8 and 1.5), 5.09 (5, ddd; 11.1, 7.8 and 1.8), 2.24 (6, dqd; 7.8, 7.0 and 2.2), 1.03 (6-Me, d; 7.0), 3.71 (7, dd; 8.3 and 2.2), 4.03(8, d; 8.3), 3.20 (8-OCH 3 , s), 7.31-7.40 (10/11/12/13/14, m); leucic acid (D) 4.86 (2, dd; 9.8 and 3.5), 1.71 (3/4, m), 1.41 (3, m), 0.89 (5/5', d; 6.4); 3-amino-2-methylpropionic acid (C) 2.71 (2, ddq; 6.8, 3.9 and 7.2), 1.21 (2-Me, d; 7.2), 3.23 (3, ddd; 13.5, 6.8 and 6.0), 3.52 (3, ddd; 13.5, 6.0 and 3.9), 6.90 (3- NH, brt; 6.0); 3-chloro-4-methoxyphenylalanine (B) 4.82 (2, ddd; 8.8, 7.4 and 5.7), 5.66 (2- NH, d; 8.8), 3.02 (3, dd; 14.4, 7.4), 3.15 (3, dd; 14.4 and 5.5), 7.23 (5, d; 2.2), 3.87 (7- OCH 3 , s), 6.84 (8, d; 8.5), 7.08 (9, dd; 8.5 and 2.2). Cryptophycin 10 To a stirred solution of 7mg of Cryptophycin 9 in lmL of acetone and 0.3mL water was added 8μL of 2N NaOH. After stirring for 4 h the solution v/as neutralized to pH 7 with IN HCl and the solvent was removed under reduced pressure. The residue was subjected to reversed-phase HPLC using 7:3 MeOH/H : O to yield pure Cryptophycin 10 (5mg). , 'H NMR (CD 3 0D): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz); 5, 7-dihydroxy-8-methoxy-6-methyl-8-phenyl-2-octenoic acid (A) 5.99 (2, dt; 15.4 and 1.3), 6.82 (3, dt; 15.4 and 7.3), 2.30 (4, m), 2.50 (4, m), 3.66 (5, td; 7.8 and 3.5), 2.05 ( d pentet; 1.8 and 7.0), 0.96 (6-Me, d; 7.0), 4.04 (7, dd; 8.8 and 2.0), 4.01 (8, d; 8.8), 3.1 (8-OCH 3 , s), 7.26-7.36 (10/11/12/13/14, m); 3-amino-2-methylpropionic acid (C) 2.50 (2, m), 1.02 (2-Me, d; 7.3), 3.16 (3, dd; 13.4 and 6.9), 3.82 (3, dd; 13.4 and 6.6); 3-chloro-4- methoxyphenylalanine (B) 4.57 (2, dd; 8.5 and 6.5), 2.82 (3, dd; 13.9 and 8.6), 3.03 (3, dd; 13.9 and 6.5), 7.25 (5, d; 2.2), 3.82 (7-OCH 3 , s), 6.96 (8, d; 8.6), 7.13 (9, dd; 8.6 and 2.2). 13 C NMR (CD 3 OD): δ 179.5, 173.4, 168.2, 155.4, 143.7, 141.7, 131.9, 131.7, 129.8, 129.3 (2C), 129.2 (2C), 128.8, 126.2, 123.2, 113.4, 85.9, 74.5, 74.1, 56.8, 56.6, 56.3, 43.3, 41.2, 40.2, 38.8, 38.0, 15.5, 9.9. Cryptophycin 12 To a solution of 5mg of Cryptophycins 1, 5 or 8 in lmL of 4: 1 acetone/water was added 15 μL of 2N NaOH. After stirring at room temperature for 5 h, the reaction mixture was neutralized to pH 7 with IN HCl and evaporated. The CH 2 Cl 2 -soluble material was passed through a small silica-cartridge with CH 2 C1 2 , 1:1 EtOAc/CH 2 Cl 2 , and EtOAc. The fraction eluted with EtOAc contained pure Cryptophycin 12. 'H NMR (CD 3 OD): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz); 5, 7,8-trihydroxy-6-methyl-8-phenyl-2-octenoic acid (A) 6.07 (A) (2, ddd; 15.5, 1.3 and 1.2), 6.40 (3, dt; 15.5 and 7.3), 2.49 (4, m), 2.60 (4, m), 3.92 (5, ddd; 9.3, 6.7 and 4.5), 1.94 (6, m), 1.07 (6-Me, d; 6.6), 3.61 (7, dd; 8.9 and 7.6), 4.56 (8, d; 7.6), 7.36 (10/14, dd; 7.4 and 1.5), 7.32 (11/13, brt; 7.5), 7.25 (12, m); 3-amino-2-methylpropionic acid (C) 2.54 (2, ddq; 7.0, 6.6 and 7.0), 1.02 (2-Me, d; 7.0), 3.14 (3, dd; 13.5 and 7.0), 3.42 (3, dd; 13.4 and 6.6); 3-chloro-4-methoxyphenylalanine (B) 4.57 (2, dd; 8.4 and 6.7), 2.83 (3, dd; 13.8 and 8.4), 3.02 (3, dd; 13.8 and 6.6), 7.25 (5, d; 2.1), 3.82 (7-OCH 3 , s), 6.95 (8, d 8.5), 7.12 (9, dd; 8.5 and 2. 1). Methylation of Cryptophycin 12 with diazomethane gave Cryptophycin 6. Cryptophycin 14 To a solution of 3 mg of Cryptophycin 6 in lmL of 3: 1 acetone/H 2 O was added 5μL of 2N NaOH. After stirring for 5 h, the reaction mixmre was neutralized to pH 7 with IN HCl and then evaporated to dryness. The residue was subjected to reversed-phase HPLC to give 2.4mg of Cryptophycin 14. Η NMR (CD 3 OD): amino or hydroxy acid unit δ (carbon position, multiplicity; J in Hz); 5-hydroxy-6-methyl-8-phenyl-2, 7-octadienoic acid (A) 5.98 (2, d; 15.3), 6.78 (3, dt; 15.3 a-nd 7.5), 2.35 (4, m), 3.64 (5, td; 7.2 and 4.8), 2.47 (6, m), 1. 14 (6-Me, d; 6.9), 6.22 (7, dd; 15.9 and 8.1), 6.39 (8, d, 15.9), 7.24-7.36 (10/11/12/13/14, m); 3-amino-2- methylpropionic acid (c) 2.35 (2, m), 1.02 (2-Me, d; 6.9), 3.18 (3, dd; 13.2 and 6.6), 3.36 (3, dd; 13.2 and 4.5); 3-chloro-4-methoxyphenylalanine (B) 4.58 (2, dd; 8.7 and 6.3), 2.80 (3, dd; 13.8 and 9.0), 3.05 (3, dd; 13.8 and 6.3), 7.25 (5, d; 2.1), 3.82 (7-OCH 3 , s), 6.95 (8, d; 8.4), 7.13(9, dd; 8.4 and 2.1). Cryptophycin 35 A catalytic amount of PtO 2 was added to a flask containing 0.5 ml of CH 2 C1 2 . The air in the flask was evacuated, H 2 was introduced, and the mixmre was stirred at room temperature for 20 min. A solution of 10 mg of Cryptophycin 1 in minimum CH 2 C1 2 was added and the mixmre was stirred at room temperature for 45 min. The catalyst was removed by filtration through celite/cotton and the solvent was evaporated. Reversed phase HPLC of the residue on a C18 column yielded 6.5 mg of Cryptophycin 35. EIMS m/z (relative intensity) 656/658 (25/10), 412/414 (25/12), 280/282 (20/10), 195/197 (78/25), 141 (58), 91 (100); high resolution EIMS m/z 656.2864 (calcd for C 35 H 45 C1NA, 0.0 mmu error); Η NMR (CDC1 3 ) amino or hydroxy acid unit _δ_values (carbon positions, multiplicities; J in Hz) 2,3-dihydro-7,8-epoxy-5-hydroxy-6-methyl-8-phenyl octanoic acid (A) 2.32 (2, ddd; 14.5, 9.2, 5.8), 2.10 (2, ddd; 14.5, 9.2, 6.2), 1.5-1.8 (3/4 overlapping m), 5.07 (5, ddd; 12.5, 5.6. 2.0), 1.80 (6, m), 1.12 (6-Me, d; 7.0), 2.90 (7, dd; 7.4, 1.8), 3.67 (8, d; 1.8), 7.24 (10/14, m), 7.32-7.38 (11/12/13, m); 3-chloro-4- methoxyphenylalanine (B) 4.71 (2, ddd; 8.7, 6.4, 6.3), 5.62 (2-NH, d; 8.7), 3.08 (2H-3, br d; 6.4), 7.19 (5, d; 2.0), 3.87 (7-OMe, s), 6.83 (8, d; 8.5), 7.07 (9, dd; 8.4, 2.0); 3-amino- 2-methylpropionic acid (Q 2.72 (2, m), 1.18 (2-Me, d; 6.9), 3.12 (3, ddd; 11.4, 10.6, 5.6), 3.70 (3, ddd), 6.76 (3-NH, br t, 6.0); leucic acid (£>) 4.83 (2, dd; 9.9, 3.8), 1.39 (3, m), 1.70 (3, m), 1.72 (4, m), 0.87 (4-Me, d; 5.3), 0.86 (5, d; 5.3); 13 C NMR (CDC1 3 ) unit δ_values (carbon positions) A 172.4 (1), 36.2 (2), 32.0 (3), 21.1 (4), 76.6 (5), 40.2 (6), 13.6 (6-Me), 63.3 (7), 59.2 (8), 136.8 (9), 125.6 (10/14), 128.7 (11/13), 128.6 (12); B 170.7 (1), 53.7 (2), 35.5 (3), 130.0 (4), 131 .1 (5), 122.2 (6), 153.8 (7), 56.1 (7-OMe), 112.1 (8), 128.5 (9); C 175.2 (1), 38.2 (2), 13.6 (2-Me), 42.1 (3); D 171.9 (1), 71.7 (2), 39.6 (3), 24.5 (4), 22.9 (4-Me), 21.4 (5). Example 10 Analysis of Microtubule Depolvmerizing Activity of Cryptophycin Materials Vinblastine, cytochalasin B, tetramethylrhodamine isothiocyanate (TRITC)-phalloidin, sulforhodamine B (SRB) and antibodies against β -tubulin and vimentin were obtained from the Sigma Chemical Company. Basal Medium Eagle containing Earle's salts (BME) was from Gibco and Fetal Bovine Serum (FBS) was purchased from Hyclone Laboratories. Cell Lines The Jurkat T cell leukemia line and A-10 rat aortic smooth muscle cells were obtained from the American Type Culmre Collection and were culmred in BME containing 10% FBS and 50μg/ml gentamycin sulfate. Human ovarian carcinoma cells (SKOV3) and a sub-line which has been selected for resistance to vinblastine (SKVLB1) were a generous gift from Dr. Victor Ling of the Ontario Cancer Institute. Both cell lines were maintained in BME containing 10% FBS and 50μg/ml gentamycin sulfate. Vinblastine was added to a final concentration of 1 μg/ml to SKVLB1 cells 24 hours after passage to maintain selection pressure for P-glycoprotein-overexpressing cells. Cell Proliferation and Cvcle Arrest Assays Cell proliferation assays were performed as described by Skehan et al. 11 For Jurkat cells, culmres were treated with the indicated drugs as described in Skehan 11 and total cell numbers were determined by counting the cells in a hemacytometer. The percentage of cells in mitosis was determined by staining with 0.4% Giemsa in PBS followed by three rapid washes with PBS. At least 1000 cells per treatment were scored for the presence of mitotic figures and the mitotic index was calculated as the ratio of cells with mitotic figures to the total number of cells counted. Immunofluorescence Assays A-10 cells were grown to near-confluency on glass coverslips in BME/10% FBS. Compounds in PBS were added to the indicated final concentrations and cells were incubated for an additional 24 hours. For the staining of microtubules and intermediate filaments, the cells were fixed with cold methanol and incubated with PBS containing 10% calf serum to block nonspecific binding sites. Cells were then incubated at 37 °C for 60 min with either monoclonal anti-β -tubulin or with monoclonal anti-vimentin at dilutions recommended by the manufacturer. Bound primary antibodies were subsequently visualized by a 45-minute incubation with fluorescein-conjugated rabbit antimouse IgG. The coverslips were mounted on microscope slides and the fluorescence patterns were examined and photographed using a Zeiss Photomicroscope 111 equipped with epifluorescence optics for fluorescein. For staining of microfilaments, cells were fixed with 3% paraformaldehyde, permeabilized with 0.2% Triton X-100 and chemically reduced with sodium borohydride (lmg/ml). PBS containing lOOnM TRITC-phalloidin was then added and the mixmre was allowed to incubate for 45 min at 37° C. The cells were washed rapidly three times with PBS before the coverslips were mounted and immediately photographed as described above. Effects of cryptophycins and vinblastine on Jurkat cell proliferation and cell cvcle Dose-response curves for the effects of cryptophycin compounds and vinblastine on cell proliferation and the percentage of cells in mitosis are indicated in Figures 2 A and 2B, respectively. Less than 3% of untreated cells displayed mitotic figures. Both the cryptophycin compounds and vmblasάne caused dose-dependent increases in the percentage of cells observed in mitosis. The increase in the mitotic index was closely correlated with decreases in cell proliferation, i.e. the concentrations of both cryptophycin compounds and vinblastine that caused 50% of the cells to accumulate in mitosis was virtually the same as the concentration which inhibited cell proliferation by 50% . The IC 50 s for the cryptophycin compounds and vinblastine for these effects were 0.2 and 8nM, respectively. Effects of cvtochalasin B. vinblastine and cryptophycins on the cytoskeleton Aortic smooth muscle (A- 10) cells were grown on glass coverslips and treated with PBS, 2μM cytochalasin B, lOOnM vinblastine or lOnM cryptophycin compounds. After 24 hours, microtubules and vimentin intermediate filaments were visualized by indirect immunofluorescence and microfilaments were stained using TRITC-phalloidin. The morphological effects of each drug were examined. Untreated cells displayed extensive microtubule networks complete with perinuclear microtubule organizing centers. Vimentin intermediate filaments were also evenly distributed throughout the cytoplasm, while bundles of microfilaments were concentrated along the major axis of the cell. Cytochalasin B caused complete depolymerization of microfilaments along with the accumulation of paracrystalline remnants. This compound did not affect the distribution of either microtubules or intermediate filaments. Both vinblastine and the cryptophycin compound caused marked depletion of microtubules. Neither compound affected microfilament organization; however, vimentin intermediate filaments collapsed, forming concentric rings around the nuclei of cells treated with either vinblastine or a cryptophycin compound. Effects of cryptophycins and vinblastine on taxol-stabilized microtubules A- 10 cells were treated for 3 hours with 0 or lOμM taxol before the addition of PBS, lOOnM vinblastine or lOnM cryptophycin compound. After 24 hours, microtubule organization was examined by immunofluorescence as described above. Compared with those in control cells, microtubules in taxol-treated cells were extensively bundled, especially in the cell polar regions. As before, vinblastine caused complete depolymerization of microtubules in non-pretreated cells. However, pretreatment with taxol prevented microtubule depolymerization in response to vinblastine. Similarly, taxol pretreatment completely stabilized microtubules against cryptophycin-induced depolymerization. Reversibility of microtubule depolymerization by vinblastine and cryptophycin A- 10 cells were treated with either lOOnM vinblastine or lOnM cryptophycins for 24 hr, resulting in complete microtubule depolymerization. The cells were then washed and incubated in drug-free medium for periods of 1 hour or 24 hours. Microtubules repolymerized rapidly after the removal of vinblastine, showing significant levels of microtubules after 1 hour and complete morphological recovery by 24 hour. In contrast, microtubules did not reappear in cells treated with cryptophycin compounds at either 1 hour or 24 hours after removal of the compound. Reversibility of cryptophycins-. vinblastine- and taxol-inhibition of cell proliferation SKOV3 cells were treated for 24 hours with previously determined IC 50 doses of vinblastine, cryptophycin compounds or taxol (i.e. values determined in experiments summarized in Table 5). During this time the cell density increased from 0.4 to 0.5 +_ 0.05 absorbance units (Figure 3), indicating a 25% increase in cell number for all three treatments. Removal of the drugs resulted in rapid growth of the vinblastine-treated cells, such that their numbers, were increased approximately 3-fold in 24 hours. In contrast, cells treated with cryptophycin compounds or taxol remained arrested, increasing only 0.2- to 0.4-fold in the 24 hours following removal of the drug. The proliferative capacity of cryptophycins or taxol-treated cells was subsequently restored since the cells then doubled in the next 24 hours. Effects of combinations of vinblastine and cryptophycins on cell proliferation SKOV3 cells were treated with combinations of cryptophycins and vinblastine for 48 hours. The percentages of surviving cells were then determined and the IC 50 s for each combination was calculated. The effects of these combinational treatments, as well as single drug treatments, are depicted as an isobologram (Figure 4). The IC 50 s for combinations of cryptophycin compounds and vinblastine fell very close to the line of additivity, indicating that these two drugs induce only additive inhibitions of cell proliferation. Toxicity of cryptophycins. vinblastine and taxol toward SKOV3 and SKVLBl cells SKVLBl cells are resistant to natural product anticancer drugs because of their over expression of P-glycoprotein 12 . The abilities of taxol, vinblastine and cryptophycin compounds to inhibit the growth of SKOV3 and SKVLBl cells are summarized in Table 5. Taxol caused dose-dependent inhibition of the proliferation of both cell lines with IC 50 s for SKOV3 and SKVLBl cells of 1 and 8000nM, respectively. Vinblastine also inhibited the growth of both cell lines, with IC 50 s of 0.35 and 4200nM for SKOV3 and SKVLBl cells, respectively. Cryptophycins demonstrated IC 50 s of 7 and 600pM for SKOV3 and SKVLBl cells, respectively. The resulting resistance factors for SKVLBl cells to the compounds are calculated as the IC 50 s for SKVLBl. IC 50 s for SKOV3 cells are also indicated in Table 5. Table 5. Cvtotoxicities of antimitotic agents for SKOV3 and SKVLBl cells Cells were treated with varying concentrations of the compounds indicated below for 48 hours. Cell numbers were then determined as indicated in the Methods section and the IC 50 for each compound was calculated. Values represent the mean + SEM for three experiments. Cell Line Compound SKOV3 SKVLB Resistance Factor IC 50 (nM) Vinblastine 0.35 ± 0.25 4200 + 1700 12,000 Taxol 1 + 0.4 8000 ± 2000 8,000 Cryptophycins 0.007 + 0.002 0.60 + 0.19 86 Thus it is demonstrated that the present invention provides novel cryptophycin compounds, as well as previously-disclosed cryptophycin compounds, which are potent inhibitors of cell proliferation, acting by disruption of the microtubule network and inhibition of mitosis. The cryptophycin compounds disrupt microtubule organization and thus normal cellular functions, including those of mitosis. Classic anti-microtubule agents, such as colchicine and Vinca alkaloids, arrest cell division at mitosis. It seemed appropriate to compare the effect of one of these agents on cell proliferation with the cryptophycin compounds. For this purpose, the Vinca alkaloid vinblastine was selected as representative of the classic anti-microtubule agents. Accordingly, the effect of cryptophycin compounds and vinblastine on the proliferation and cell cycle progression of the Jurkat T-cell leukemia cell line was compared. Both compounds caused parallel dose-dependent inhibitions of cell proliferation and accumulation of cells in mitosis. Since antimitotic effects are commonly mediated by disruption of micrombules in the mitotic spindles, the effects of cryptophycin compounds on cytoskeletal structures were characterized by fluorescence microscopy. Immunofluorescence staining of cells treated with either a cryptophycin compound or vinblastine clearly demonstrated that both compounds caused the complete loss of micrombules. Similar smdies with SKOV3 cells demonstrate that the anti-microtubule effects of cryptophycin compounds are not unique to the smooth muscle cell line. Neither drug affected the levels or distribution of microfilament bundles, as was readily induced by cytochalasin B, indicating that the loss of micrombules may not be due to a non-specific mechanism, e.g. activation of proteases or loss of energy charge. Both vinblastine and cryptophycin compounds also promote marked collapse of vimentin intermediate filaments, such that brightly staining rings were formed around the cell nucleus. Removal of vinblastine from the culmre medium resulted in rapid repolymerization of micrombules. In contrast, cells treated with cryptophycin compounds remained depleted of microtubules for at least 24 hours after the compound was removed from the culmres. The present invention demonstrates that cryptophycin compounds circumvent P-glycoprotein-mediated multiple drug resistance. Transport by P-glycoprotein limits the ability of namral product anticancer drugs to inhibit the growth of tumor cells with acquired or de novo drug resistance. 13 15 Vinca alkaloids, while very useful in the initial course of chemotherapy, are extremely good substrates for transport by P-glycoprotein, and so are of very limited usefulness against P-glycoprotein-mediated MDR tumors. Therefore, identification of agents which overcome multiple drug resistance may, should lead to the development of useful and novel anticancer agents. The cryptophycin compounds of the present invention appear to be such agents since they are poor substrates for P-glycoprotein- mediated transport. This fact is reflected in the low cell resistance factor for cryptophycin compounds compared with vinblastine, taxol and other namral product drugs. All publications and patent applications cited in this specification, but not individually and specifically incorporated by reference, are herein incorporated by reference as if they had been specifically and individually indicated to be incorporated by reference. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity and understanding, it will be apparent to those of ordinary skill in the art in light of the teaching of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the claims. References I. Eglof, G., Organic Chemistry: An Advanced Treatise. Gilmar et al. (ed.), pp. 31- 46, John Wiley & Sons (1943). 2. Kemp, et al. , Organic Chemistry. Worth Publishers, Inc. (1980). 3. Patterson, G. M. L. et al. J. Phvcol. 27:530-6 (1991). 4. Corbett, T.H. et al. Cytotoxic Anticancer Drugs: Models and Concepts for Drug Discovery and Development, pp 35-87; Kluwer Academic Publishers: Norwell, 1992. 5. Valeriote, F.A. et al. Discovery and Development of Anticancer Agents: Kluwer Academic Publishers: Norwell, 1993; in press. 6. Schwartz, R.E. et al. J. Ind. Microbiol. 5: 113-24 (1990). 7. Hirsch, CF. et al. U.S. Patent 4,946,835, issued August 7, 1990. 8. Sesin, D.F. U.S. Patent 4,845,085, issued July 4, 1989. 9. Sesin, D.F.; Liesch, J.M. U.S. Patent 4,868,208, issued September 19, 1989. 10. Sesin, D.F. U.S. Patent 4,845,086, issued July 4, 1989. I I. Skehan, P. et al.J. Natl. Cancer Inst. 82: 1107-1112 (1990). 12. Bradley, G. et al. Cancer Res. 49: 2790-2796 (1989). 13. Endicott, J.A. et al. Ann. Rev. Biochem. 58: 137-171 (1989). 14. Beck, W.T. Biochem. Pharm. 36: 2879-2887 (1987). 15. Moscow, J.A. et al. J. Natl. Cancer Inst. 80: 14-20 (1988).

Claims: What is claimed is: 1. A compound represented by the strucmre: Wherein R, is H, OH, a halogen, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; or Rj and R 2 may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or a second bond between C 10 and C„; or R, and R, may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R 4 is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; R; is H or an OH group; -R e is H; or Rj and R € may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxy benzyl, methoxybenzyl, halohydroxy benzyl, dihalohydroxy benzyl, halomethoxybenzyl, or dihalomethoxybenzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterified lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R 4 and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; or R j and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; or with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R 2 is OH, O of a ketone group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone group, NH 2 , SH; when R, is OH, R 2 is OH, R 3 is methyl, R^ and R f , are taken together to form a second bond between C 5 and C 6 , R 4 and R 8 are taken together to form the didepsipeptide group with the structure X: wherein O, of X corresponds to R,, N 8 of X corresponds to R 8 , -R, is methyl, and R 10 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; when R, and R 2 are taken together to form an epoxide ring, R 3 is methyl, Rj and R^ are taken together to form a second bond between C 5 and C 6 , R 4 and R 8 are taken together to form a didepsipeptide with the strucmre X, R, is methyl, and R 10 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; when R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 is methyl, Rj and R^ are taken together to form a second bond between C 5 and C 6 , R 4 and R 8 are taken together to form a didepsipeptide with the strucmre X, R, is methyl, and R 10 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; and when R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R, and R f , are taken together to form a second bond between C, and C 6 , R 4 is bonded to the carboxy terminus of leucic acid, and R 8 is bonded to the nitrogen terminus of either 3- amino-2-methylpropionic acid or 3-amino-2-methylpropionic acid methyl ester, R 7 is not 3-chloro-4-methoxybenzyl. 2. The compound of claim 1, wherein at least one of the groups attached to C 2 , C 8 , C 9 , C I0 , and C u have R stereochemistry. 3. The compound of claim 1, wherein at least one of the groups attached to C 2 , C 8 , C 9 , C 10 , and C„ have S stereochemistry. 4. The compound of claim 1, wherein the strucmre of the didepsipeptide that is formed when R 4 is taken together with R 8 is the following structure X: wherein OI of X corresponds to R, and N 8 of X corresponds to R 8 . 5. The compound of claim 1 , wherein the strucmre of the didepsipeptide that is formed when R5 is taken together with R 8 is the following strucmre X: wherein OI of X corresponds to R 5 and N 8 of X corresponds to R 8 . 6. The compound of claim 1, wherein the lower α-hydroxy alkanoic acid of R 4 is leucic acid or an isomer thereof. 7. The compound of claim 1, wherein R 7 is a 4-methoxybenzyl group. 8. The compound of claim 6, wherein the R 7 is a 3-halo-4-methoxybenzyl group. 9. The compound of claim 1, wherein R, and R, are taken together to form a tetrahydrofuran ring, R 2 is OH, R 3 is methyl, Rj and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 8 is a (2- carbomethoxypropyl)amino group. 10. The compound of claim 1, wherein R^ and R 4 are taken together to form a tetrahydrofuran ring, R 2 and R 8 are OH R 3 is methyl, ^ and R f , are taken together to form a second bond between C 5 and C 6 , and R 7 is 3-chloro-4-methoxybenzyl. 11. The compound of claim 1, wherein R, is OCH 3 , R 2 and R 4 are OH, R 3 is methyl, R j and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4- methoxybenzyl, and R 8 is (2-carboxypropyl)amino. 12. The compound of claim 1, wherein R, and R 4 are taken together to form a tetrahydrofuran ring, R 2 is OH, R 3 is methyl, -R, and -R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 8 is (2-carboxypropyl)amino. 13. The compound of claim 1, wherein R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 is methyl, R 4 is OH, Rj and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 8 is (2- carboxypropyl)amino. 14. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, -R, and -R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 4-methoxybenzyl, and R, and R 8 are taken together to form the strucmre X where R, is methyl and R I0 is isobutyl. 15. The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between the C 10 and C„ carbons, R 3 is methyl, R 5 and R« are taken together to form a second bond between C s and C 6 , R 7 is 4-methoxybenzyl, and R, and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 16. The compound of claim 4, wherein Ri is a chloro group, R 2 is OH, R 3 is methyl, R j and R « are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4- methoxybenzyl, and R, and R 8 are taken together to form the structure X where R, is methyl and R 10 is isobutyl. 17. The compound of claim 4, wherein R, is OCH 3 , R 2 is an OH group, R 3 is methyl, R 5 and R^ are taken together to form a second bond between C 5 and C 6 such that there is a double bond, R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 18. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R j and -^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro~4-hydroxybenzyl, and R 4 and R s are taken together to form the structure X where R, is methyl and R 10 is isobutyl. 19. The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between C I0 and C π , R 3 is methyl, Rj and R < , are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-hydroxybenzyl, and R, and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 20. The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between C, 0 and C„, R 3 is methyl, R 5 and R « are taken together to form a second bond between C 5 and C 6 , R 7 is 3 -chloro-4-methoxy benzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is sec-butyl. 21. The compound of claim 4, wherein R, and R, are taken together to form a second bond between C 10 and C„, R 3 is methyl, R 5 and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R l3 is isopropyl. 22. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, Rj and 1^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the structure X where R, is hydrogen and R 10 is isobutyl. 23. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 and 1^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3,5-dichloro-4-hydroxybenzyl, and R 4 and R 8 are taken together to form the structure X where R, is methyl and R 10 is isobutyl. 24. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R-, and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 4-methoxybenzyl, and R, and R 8 are taken together to form the strucmre X where R, is hydrogen and R 10 is isobutyl. 25. The compound of claim 5, wherein R, and R 2 are taken together to form a second bond between the C 10 and C„ carbons, R 3 is methyl, R, is hydroxy, R^ is H, R 7 is 3-chloro-4- methoxy benzyl, and R^ and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 26. The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 is hydrogen, Rj and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 27. The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 is methyl, Rj and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is H and R 10 is isobutyl. 28. • The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between C, 0 and C„, R 3 is methyl, Rj is OH, R is H, R 7 is 3-chloro-4-methoxybenzyl, and R, and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 29. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 and R are taken together to form a second bond between C 5 and C 6 , R 7 is 3,5-dichloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R 9 is methyl and R 10 is isobutyl. 30. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R, and R^ are H, R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 31. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is H, R 5 and R^, are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is isobutyl. 32. The compound of claim 4, wherein R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 is methyl, R 5 and -R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3,5-dichloro-4-hydroxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R-, is methyl and R 10 is isobutyl. 33. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, Rj and 1^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R, and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is propyl. 34. The compound of claim 4, wherein R t and R, are taken together to form a second bond between C, 0 and C„, R 3 is methyl, R 5 and R f , are taken together to form a second bond between C 5 and C 6 , R 7 is 3-chloro-4-methoxybenzyl, and R 4 and R 8 are taken together to form the strucmre X where R, is methyl and R 10 is propyl. 35. The compound of claim 4, wherein R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R s and R^ are taken together to form a second bond between C 5 and C 6 , R 7 is 3 -chloro-4-methoxy benzyl, and R 4 and R 8 are taken together to form the strucmre X where -R, is methyl and R 10 is sec-butyl. 36. A method for producing a compound in accordance with claim 1 comprising a) cultivating a culture of a Nostoc sp. of cyanobacteria; and b) isolating from the culture at least one compound in accordance with claim 1. 37. The method of claim 36 further comprising chemically modifying the compound obtained in step (b) to provide a distinct compound in accordance with claim 1. 38. The method of claim 36, wherein the Nostoc sp. is designated GSV 224 (ATCC No. 55483). 39. A method for producing a compound with the following strucmre: Wherein R, is H, OH, a halogen, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; or R, and R 2 may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or a second bond between C 10 and C„; or R, and R 4 may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R, is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; R- 5 is H or an OH group; R e is H; or R, and R^ may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxybenzyl, methoxybenzyl, halohydroxy benzyl, dihalohydroxybenzyl, halomethoxybenzyl, or dihalomethoxybenzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterified lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R, and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; or -R s and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R 2 is OH, O of a ketone group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone group, NH 2 , SH, the method comprising a) cultivating a culmre of a Nostoc sp. of cyanobacteria designated GSV 224 (ATCC No. 55483); b) isolating from the culmre at least one compound having the strucmre. 40. The method of claim 39 further comprising chemically modifying the compound obtained in step (b) to provide a distinct compound having the strucmre. 41. A pharmaceutical composition useful for inhibiting the proliferation of a hyperproliferative mammalian cell comprising an effective amount of a compound with the following strucmre: Wherein R, is H, OH, a halogen, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; or R ! and R 2 may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or a second bond between C 10 and C„; or R, and R 4 may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R, is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; R s is H or an OH group; R e is H; or Rj and - g may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxybenzyl, methoxybenzyl, halohydroxy benzyl, dihalohydroxybenzyl, halomethoxybenzyl, or dihalomethoxy benzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterified lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R, and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; or R 5 and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; or with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R 2 is OH, O of a ketone group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone group, NH 2 , SH; when R, is OH, R 2 is OH, R 3 is methyl, I , and R^ are taken together to form a second bond between C 5 and C 6 , R 4 and R 8 are taken together to form the didepsipeptide group with the strucmre X: wherein O, of X corresponds to R,, N 8 of X corresponds to R 8 , -R, is methyl, and R I0 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; when R, and R 2 are taken together to form an epoxide ring, R 3 is methyl, R j and R^ are taken together to form a second bond between C 5 and C 6 , R, and R 8 are taken together to form a didepsipeptide with the strucmre X, R, is methyl, and R 10 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; when R, and R 2 are taken together to form a second bond between C 10 and C„, R 3 is methyl, R 5 and R * are taken together to form a second bond between C 5 and C 6 , R, and R 8 are taken together to form a didepsipeptide with the strucmre X, -R, is methyl, and R I0 is isobutyl, R 7 is not 3-chloro-4-methoxybenzyl; and when R, and R 2 are taken together to form an epoxide group, R 3 is methyl, R 5 and 1^ are taken together to form a second bond between C 5 and C 6 , R, is bonded to the carboxy terminus of leucic acid, and R 8 is bonded to the nitrogen terminus of either 3- amino-2-methylpropionic acid or 3-amino-2-methylpropionic acid methyl ester, R 7 is not 3-chloro-4-methoxybenzyl, together with a pharmaceutically acceptable carrier. 42. The pharmaceutical composition of claim 41 further comprising at least one additional anti-neoplastic agent. 43. The composition of claim 42, wherein the anti-neoplastic agent is an antimetabolite. 44. The composition of claim 42, wherein the anti-neoplastic agent is an alkylating agent. 45. The composition of claim 42, wherein the anti-neoplastic agent is a plant alkaloid. 46. The composition of claim 42, wherein the anti-neoplastic agent is an antibiotic. 47. The composition of claim 42, wherein the anti-neoplastic agent is a hormone. 48. The composition of claim 42, wherein the anti-neoplastic agent is an enzyme. 49. The composition of claim 42, wherein the anti-neoplastic agent is an adrenocortical suppressant. 50. The composition of claim 42, wherein the anti-neoplastic agent is an aminoacridine derivative. 51. A method for inhibiting the proliferation of a mammalian cell comprising contacting the mammalian cell with a cryptophycin compound in an amount sufficient to inhibit the proliferation of the cell. 52. The method of claim 51 , wherein the cryptophycin compound has the following strucmre: —to— Wherein R, is H, OH, a halogen, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; or R, and R 2 may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or a second bond between C 10 and C u ; or R, and R, may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R 4 is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; Rj is H or an OH group; R t is H; or R 5 and R s may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxybenzyl, methoxybenzyl, halohydroxybenzyl, dihalohydroxybenzyl, halomethoxy benzyl, or dihalomethoxybenzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterified lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R 4 and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; and R 5 and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; and with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R 2 is OH, O of a ketone group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone group, NH 2 , SH. 53. The method of claim 51 further comprising contacting the cell with at least one additional anti-neoplastic agent. 54. The method of claim 53, wherein the anti-neoplastic agent is an antimetabolite. 55. The method of claim 53, wherein the anti-neoplastic agent is an alkylating agent. 56. The method of claim 53, wherein the anti-neoplastic agent is a plant alkaloid. 57. The method of claim 53, wherein the anti-neoplastic agent is an antibiotic. 58. The method of claim 53, wherein the anti-neoplastic agent is an enzyme. 59. The method of claim 53, wherein the anti-neoplastic agent is an adrenocortical suppressant. 60. The method of claim 53, wherein the anti-neoplastic agent is an aminoacridine derivative. 61. The method of claim 51, wherein the mammalian cell is hyperproliferative. 62. The method of claim 61, wherein the hyperproliferative cell is human. 63. A method of inhibiting the proliferation of a hyperproliferative mammalian cell having a multiple drug resistant phenotype comprising contacting the cell with an amount of a cryptophycin compound effective to disrupt the dynamic state of micrombule polymerization and depolymerization to arrest cell mitosis, thereby inhibiting the proliferation of the cell. 64. The method of claim 63, wherein the cryptophycin compound has the following structure: Wherein R, is H, OH, a halogen, O of a ketone group, NH,, SH, a lower alkoxyl group or a lower alkyl group; R 2 is H, OH, O of a ketone group, NH 2 , SH, a lower alkoxyl group or a lower alkyl group; or R, and R 2 may be taken together to form an epoxide ring, an aziridene ring, a sulfide ring or a second bond between C 10 and C„; or R, and R 4 may be taken together to form a tetrahydrofuran ring; R 3 is H or a lower alkyl group; R 4 is OH, a lower alkanoyloxy group or a lower α-hydroxy alkanoyloxy group; R j is H or an OH group; -R e is H; or Rj and ^ may be taken together to form a second bond between C 5 and C 6 ; R 7 is a benzyl, hydroxybenzyl, methoxybenzyl, halohydroxy benzyl, dihalohydroxybenzyl, halomethoxybenzyl, or dihalomethoxybenzyl group; R 8 is OH, a lower β-amino acid wherein C, is bonded to N of the β-amino acid, or an esterified lower β-amino acid wherein C, is bonded to N of the esterified lower β-amino acid group; R 4 and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; or - j and R 8 may be taken together to form a didepsipeptide group consisting of a lower β- amino acid bonded to a lower α-hydroxy alkanoic acid; with the following provisos: R, is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone group, NH 2 , SH; R 2 is H, a lower alkyl group, or a lower alkoxyl group only if R, is OH, O of a ketone group, NH 2 , SH. 65. The method of claim 63 further comprising contacting the cell with at least one additional anti-neoplastic agent. 66. The method of claim 65, wherein the anti-neoplastic agent is an antimetabolite. 67. The method of claim 65, wherein the anti-neoplastic agent is an alkylating agent. 68. The method of claim 65, wherein the anti-neoplastic agent is a plant alkaloid. 69. The method of claim 65, wherein the anti-neoplastic agent is an antibiotic. 70. The method of claim 65, wherein the anti-neoplastic agent is an enzyme. 71. The method of claim 65, wherein the anti-neoplastic agent is an adrenocortical suppressant. 72. The method of claim 65, wherein the anti-neoplastic agent is an aminoacridine derivative. 73. The method of claim 63, wherein the mammalian cell is human. 74. A method of alleviating a pathological condition caused by hyperproliferating mammalian cells comprising administering to a subject an effective amount of the pharmaceutical composition of claim 41 to inhibit proliferation of the cells. 75. The method of claim 74 further comprising administering to the subject at least one additional therapy directed to alleviating the pathological condition. 76. The method of claim 75, wherein the pathological condition is characterized by the formation of neoplasms. 77. The method of claim 76, wherein the neoplasms are selected from the group consisting of mammory, small-cell lung, non-small-cell lung, colorectal, leukemia, melanoma, pancreatic adenocarcinoma, central nervous system (CNS), ovarian, prostate, sarcoma of soft tissue or bone, head and neck, gastric which includes pancreatic and esophageal, stomach, myeloma, bladder, renal, neuroendocrine which includes thyroid and non-Hodgkin' s disease and Hodgkin's disease neoplasms. 78. The method of claim 74, wherein the mammalian cells are human.

Inventors: CORBETT THOMAS H, MOOBERRY SUSAN L, MOORE RICHARD E, PATTERSON GREGORY M L, SMITH CHARLES D, VALERIOTE FREDERICK A, CORBETT, THOMAS, H., MOOBERRY, SUSAN, L., MOORE, RICHARD, E., PATTERSON, GREGORY, M., L., SMITH, CHARLES, D., VALERIOTE, FREDERICK, A.
Applicants: CORBETT THOMAS H, MOOBERRY SUSAN L, MOORE RICHARD E, PATTERSON GREGORY M L, SMITH CHARLES D, UNIV HAWAII, VALERIOTE FREDERICK A, WAYNE STATE UNIVERSTY, CORBETT, THOMAS, H., MOOBERRY, SUSAN, L., MOORE, RICHARD, E., PATTERSON, GREGORY, M., L., SMITH, CHARLES, D., UNIVERSITY OF HAWAII, VALERIOTE, FREDERICK, A.
Application year: 1994
Main classification: A01N37
IPC codes: A61K31, A61K38, A61K45, A61P35, A61P43, C07C237, C07D273, C07D303, C07D307, C07D413, C07J21, C12P13, C12P21