alpha -1-ANTICHYMOTRYPSIN ANALOGUES HAVING CHYMASE INHIBITING ACTIVITY

Abstract: The invention provides analogues of alpha -1-antichymotrypsin having amino acid substitutions at positon 358. alpha -1-antichymotrypsin analogues having amino acid substitutions at positions 356-361 and analogues having amino acid substitutions at positions 356-361 wherein the amino acid at position 358 is substituted are also within the scope of the invention. These analogues exhibit chymase inhibitory activity. Also provided are novel alpha -1-antichymotrypsins having an N-terminal extension of methionine-alanine-serine or alanine-serine. Expression vectors for the production of alpha -1-antichymotrypsins are also provided. The present invention also provides host cells and cell cultures capable of expressing analogues of alpha -1-antichymotrypsin, as well as protein preparations from the host cells. Methods of producing and using the analogues of alpha -1-antichymotrypsin to inhibit chymase activity are also provided.

Description: <Desc/Clms Page number 1> a-1-ANTICHYMOTRYPSIN ANALOGUES HAVING CHYMASE INHIBITING ACTIVITY REFERENCE TO RELATED APPLICATIONS The present application is a continuation-in-part of U.S. Patent Application Serial No. UPN-0964, filed concurrently herewith, the concurrently filed applications are continuations-in-part of U. S. Patent Application Serial No. 08/005,898, and U.S. Patent Application Serial No. 08/005,908, filed January 15,1993, which are divisionals of U.S. Patent Application Serial No. 735,322, filed July 24,1991, now U. S. Patent 5,266,465, which is in turn a divisional application of U.S. Patent Application Serial No. 370,704 filed June 23,1989, which is now U. S. Patent 5,079,336. The disclosures of each of these applications is hereby incorporated by reference. REFERENCE TO GOVERNMENT GRANTS The work for the present invention was supported in part by National Institutes of Health grants AG-10599 and AR-39674. The United States government may have certain rights in the invention. FIELD OF THE INVENTION This invention is related to the field of substances produced by recombinant DNA technology and more particularly to the field of proteins produced by recombinant DNA technology. BACKGROUND OF THE INVENTION <Desc/Clms Page number 2> The interaction of serine protease inhibitors, serpins (serpin - serine proteinase inhibitor), with endogenous and microbial proteases produces a spectrum of molecular species each of which are components of a highly evolutionarily conserved homeostatic mechanism that operates to maintain a concentration of intact, active serpins pivotal in host survival. Of these species, the serpin-enzyme complex and the hydrolyzed, inactive form of the intact serpin stimulate the production of IL-6, signaling hepatocytes to increase synthesis of the acute phase proteins including a subpopulation of the serpin superfamily of proteins. Although serpin-enzyme complexes are rapidly cleared from the circulation, they may accumulate along with the cleaved and intact forms in local areas of inflammation. This establishes a complex microenvironment of chemoattractants and inhibitors of chemotaxis as well as activators and inhibitors of neutrophil degranulation, leukotriene, platelet activating factor (PAF) and superoxide production. Kilpatrick et al. have shown that native antichymotrypsin (ACT) and recombinant antichymotrypsin (rACT) inhibited superoxide generation by human neutrophils in suspension. Kilpatrick et al., J. Immunol. , 1991,146, 2388. In this system, intact ACT and rACT complexed with chymotrypsin (Chtr) were equally effective in inhibiting free radical production by neutrophils stimulated with f-Met-Leu-Phe, Concanavalin A (ConA) or phorbol myristate acetate (PMA). A variety of animal models corroborate the hypothesis that runaway serine protease activity is a major mechanism of lung injury and that an appropriate serpin response controls the degree of the injury. For example, antithrombin III (ATIII) in combination with alpha-1protease inhibitor (alPI), protected sheep from endotoxininduced lung injury where the individual serpins were not as effective as the combination. Redens et al., Circ. Shock, 1988, 26, 15. Redens et al. also showed that ATIII protects against the development of disseminated <Desc/Clms Page number 3> intravascular coagulation in endotoxemic rats. Emerson et al . , Circ. Shock, 1987, 21, 1. A scavenger of H2O2 and a chloromethyl ketone inhibitor of elastase blocked reactive oxygen potentiation of neutrophil elastase-mediated acute edematous lung injury in a rat and alPI diminished bleomycin-mediated pulmonary inflammation as well as subsequent fibrosis. Baird et al., Physiol., 1986, 61, 2224 and Nagai et al., Am. Rev. Resp. Dis. , 1992, 145, 651. While in another system, however, neutrophil elastase inhibitors, Eglin C and a low molecular weight compound L 658,758, failed to inhibit leukotriene B4 (LTB4) -induced neutrophil-mediated adherence, diapedesis or vascular leakage. Rosengren et al., Am. J. Physiol., 1990, 259, H1288. Accordingly, inhibitors of proteolytic enzymes administered therapeutically may limit the molecular and cellular mechanisms of inflammation and reduce tissue damage. Therefore, there remains a need for safe and effective inhibitors such as a-1-antichymotrypsin for clinical applications in animals. Therapeutic agents based on multifunctional protease inhibitors will clinically advance therapy of diseases where free radicals as well as proteases have been implicated in the mechanism of injury such as the adult respiratory syndrome, pancreatitis, inflammatory skin lesions and reperfusion injury. SUMMARY OF THE INVENTION The present invention provides analogues of human a-1-antichymotrypsin having antichymotrypsin activity and/or chymase inhibitory activity. The present invention includes a-1-antichymotrypsin analogues having an amino acid substitution at position 358 such that the wild type position 358, leucine, is changed to an amino acid selected from tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. Also included in <Desc/Clms Page number 4> the scope of the present invention is an analogue of human a-1-antichymotrypsin wherein the amino acids corresponding to the amino acids Thr-Leu-Leu-Ser-Ala-Leu (SEQ ID NO: 7) at positions 356 to 361 of wild-type a-1-antichymotrypsin are substituted with amino acids Ile-Pro-Xxx-Ser-Ile-Pro (SEQ ID NO: 8). SEQ ID NO: 8 may also be substituted at position 358 with an amino acid selected from the group set forth above. The novel polypeptides indicated above, as well as human wild type a-1-antichymotrypsin, Figure 1, may have an N-terminal extension sequence of methionine-alanineserine or alanine-serine. The novel polypeptides having an N-terminal extension are produced from the nucleotide sequence encoding the entire amino acid sequence of mature human wild type a-1-antichymotrypsin, optionally having one of the substitutions indicated above, with an N-terminal extension sequence of methionine-alanine-serine or alanineserine. These novel a-1-antichymotrypsin analogues result in stable a-1-antichymotrypsin monomers and eliminates an N-terminal cysteine present in the rACT described precursor a-1-antichymotrypsin polypeptide that gave rise to protein dimers and apparent heterogeneity. These a-1- antichymotrypsin polypeptides have antichymotrypsin activity and are useful in the same manner as a-1antichymotrypsin itself. The analogues of the present invention are also useful for chymase inhibition. The present invention also provides expression vectors comprising nucleic acid sequences coding for the a- 1-antichymotrypsin analogues of the invention, transformed host cells and cell cultures capable of expressing the analogues of the invention. The present invention further provides methods of producing a-1-antichymotrypsin analogues comprising culturing a host cell capable of expressing a-1-antichymotrypsin analogues. The present invention also provides methods of inhibiting chymase comprising contacting chymase with an <Desc/Clms Page number 5> inhibitory amount of at least one a-1-antichymotrypsin analogue. A method of using a-1-antichymotrypsin analogues to inhibit chymase activity comprising adding an effective amount of an a-1-antichymotrypsin analogue of the invention to a sample exhibiting chymase activity is also provided. The scope of the present invention provides analogues of human wild type a-1-antichymotrypsin which are useful in the treatment of lung inflammation, reperfusion injury, and treating and preventing blood clots. Medicaments and compositions comprising analogues of human wild type a-1-antichymotrypsin in pharmaceutically acceptable amounts together with a carrier are also the subject of the present invention, as are their uses in treating the conditions identified above. This invention is more particularly pointed out in the appended claims and is described in its preferred embodiments in the following description. BRIEF DESCRIPTION OF THE FIGURES Figure 1 shows the complete nucleotide sequence and predicted amino acid sequence of human wild type a-1antichymotrypsin. The full length gene is encoded by nucleotide residues 1-1209. Figure 2 is a graph of the titration of human chymase with a-1-antichymotrypsin analogues. DETAILED DESCRIPTION OF THE INVENTION The present invention provides analogues of human a-1-antichymotrypsin wherein the amino acid corresponding to leucine at amino acid position 358 of wild type human a- 1-antichymotrypsin is substituted with an amino acid selected from tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. In a preferred embodiment of the invention, leucine at position 358 is substituted with tryptophan. <Desc/Clms Page number 6> An analogue of a-1-antichymotrypsin wherein amino acids Thr-Leu-Leu-Ser-Ala-Leu (SEQ ID NO: 7) at positions 356-361 are substituted with Ile-Pro-Xxx-Ser-Ile-Pro (SEQ ID NO: 8) is also provided by the present invention. The analogues having amino acid substitutions at positions 356- 361 may be substituted at position 358 with an amino acid selected from the group consisting of methionine, tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. Wild type antichymotrypsin having alterations in the amino acid leucine at position 358 results in analogues referred to as rACT-L358 followed by the one letter amino acid symbol substituted in a particular analogue. For example, rACT-L358W represents recombinant a-1antichymotrypsin having tryptophan at amino acid position 358. Another aspect of the invention provides human a- 1-antichymotrypsin, in wild type and analogue forms, having an N-terminal extension of Met-Ala-Ser. In this embodiment of the invention, mature human a-1-antichymotrypsin contains three additional amino acids at the N-terminus, Met-Ala-Ser. Another embodiment comprises mature human a- 1-antichymotrypsin, in wild type and analogue forms, with Met cleaved and two amino acids, Ala-Ser, remaining at the N-terminus. Wild type and analogue forms of a-1antichymotrypsin may have the N terminal extensions disclosed herein. a-1-Antichymotrypsin is a serine protease inhibitor (serpin). In its native, circulating form, it is a glycoprotein of between 55,000 and 66,000 daltons, with the variation attributed to microheterogeneity in glycosylation. It is synthesized predominantly in the liver and has also been reported in mast cells, sinus histiocytes, endothelial cells and in cells of the histio/monocytic line. In response to inflammatory <Desc/Clms Page number 7> stimuli, plasma levels of a-1-antichymotrypsin increase more than four-fold within several hours. Chymotrypsin-like enzymes and their inhibitors have been identified in a wide variety of normal and abnormal biological @@@ocesses including modulation of cellular functions, DNA-binding, inhibition of certain parasite functions and processing of vasoconstrictor proteins. In addition, a-1-antichymotrypsin appears to be a component of the amyloid deposit in Alzheimer's plaques and is present in various carcinomas and in some tissues of the reproductive system. Human a-1-antichymotrypsin forms sodium dodecyl sulfate (SDS) -stable complexes with its target enzymes, which is a general property of serpin/serine protease interactions. Very little is known about the nature of these complexes. Although high-resolution crystal structures of chymotrypsin and chymotrypsin/small molecular inhibitor complexes have been resolved and NMR analyses of the enzyme have been reported, no direct structural studies of human a-1-antichymotrypsin alone or as a complex with a serine protease have been reported. There is evidence that proteases and oxidants play a central role in establishing and maintaining shock physiology and that protease inhibitors can favorably modify the outcome of shock. Small molecule protease inhibitors have been shown to have efficacy in pancreatitis in humans. Similarly, antichymotrypsins may be implicated in treatment of coagulation disorders as in liver diseases. Proteases are also important mediators of inflammatory diseases. Regulation of these enzymes by their inhibitors is critical for the control of tissue destruction in these diseases. The analogues of the invention, having an amino acid substitution at amino acid position 358, the analogues having amino acids substitutions at amino acid positions 356-361 of wild-type a-1-antichymotrypsin with position 358 having an amino acid selected from the amino acids set <Desc/Clms Page number 8> forth above, are surprisingly and unexpectedly much better inhibitors of chymase, a proteinase stored at high concentrations within human mast cells, than wild-type or native antichymotrypsin. Human chymase is inhibited by two human serpin plasma inhibitors, a-1-antichymotrypsin and a- 1-proteinase inhibitor. Both of these inhibitors are members of the serpin protein family. Although inhibition of chymase appeared irreversible with a-1-antichymotrypsin and a-1-proteinase inhibitor, showing the typical tightbinding complex resistant to denaturation in SDS, reactions with both inhibitors were not highly efficient. Determined under pseudo first order conditions, second order rate constants of 25,000 and 8,000 M-1S-1 were obtained for a-1antichymotrypsin and a-1-proteinase inhibitor, respectively. These values are approximately 100 to 1,000 fold lower than the values observed for the inhibition of neutrophil cathepsin G by a-1-antichymotrypsin, or the inhibition of neutrophil elastase by a-1-proteinase inhibitor. The inefficiency of the reaction was even more evident in titrations where endpoints yielded stoichiometries of inhibition (SI) of 4.5 (a-1antichymotrypsin) and 5.0 (a-1-proteinase inhibitor) moles of inhibitor/mole of chymase. Analysis of these reactions by SDS-PAGE and by N-terminal sequence analysis of products indicated that the high stoichiometries of inhibition were due to a competing reaction producing a degraded inhibitor by hydrolysis within the reactive loop of the inhibitor. The a-1-antichymotrypsin analogue wherein the leucine at position 358 of wild-type a-1-antichymotrypsin is substituted with tryptophan is a much more efficient inhibitor of chymase than the natural inhibitors. The stoichiometry of inhibition of approximately 1 mole of inhibitor/mole of chymase. Thus, the competing cleavage reaction is nearly eliminated, indicating that the analogues of the invention are four-fold more efficient for inhibition of chymase than wild type a-1-antichymotrypsin. The apparent second order rate constant for inhibition is <Desc/Clms Page number 9> more than five-fold greater than the apparent second order rate constant for inhibition of chymase by wild type a-1antichymotrypsin, rACT, demonstrating a faster interaction. Another aspect of the present invention provides nucleic acid sequences coding for a-1-antichymotrypsin and analogues, expression vectors and host cells transformed to express the nucleic acid sequences of the invention, protein preparations comprising the proteins of the present invention synthesized in a host cell transformed with a DNA sequence encoding the protein and cell cultures capable of expressing the nucleic acid sequence of the invention. Generally, a-1-antichymotrypsin analogues, having amino acid substitutions at amino acid position 358 or having amino acid substitutions at amino acid positions 356-361 in the wild-type a-1-antichymotrypsin with position 358 having an amino acid selected from the amino acids identified above, are produced in host cells that have been transformed with an expression vector comprising a nucleic acid sequence coding for the particular protein. The host cells are cultured under conditions whereby the nucleic acid sequence coding for the particular protein is expressed. After a suitable amount of time for the protein to accumulate, the protein is purified from the host cells or medium surrounding the cells. A human gene coding for a-1-antichymotrypsin can be readily obtained from a human liver cDNA library. Suitable libraries can be obtained from commercial sources such as Clontech, Palo Alto, CA. Positive clones are then subjected to DNA sequencing to determine the presence of a DNA sequence coding for a-1-antichymotrypsin. DNA sequencing is readily accomplished using the chain termination method of Sanger et al., Proc. Nat'l. Acad. Sci, U.S.A., 1977,74, 5463. The DNA sequence coding for a-1-antichymotrypsin may then be manipulated using the polymerase chain reaction, PCR, or alternatively, inserted into a cassette vector, as disclosed in U.S. Patent 5,079,336, the <Desc/Clms Page number 10> disclosures of which is hereby incorporated by reference. Expression vectors, host cells and cell cultures suitable for use in the invention are chosen from expression systems capable of synthesizing a-1antichymotrypsin analogue products. Host cells suitable for use in the invention include prokaryotic and eukaryotic cells that can be transformed to stably contain and express a-1-antichymotrypsin, as disclosed in U. S. Patent 5,079,336, incorporated herein by reference. Suitable types of cells for practicing the present invention include bacterial, yeast and mammalian cells. Nucleic acids coding for recombinant a-1antichymotrypsin may be expressed in prokaryotic or eukaryotic host cells, including the most commonly used bacterial host cell for the production of recombinant proteins, E. coli. Other microbial strains may also be used, such as Bacillus subtilis and other enterobacteriaceae such as Salmonella typhimurium or Serratia marcescens, various species of Pseudomonas or other bacterial strains. Commonly used eukaryotic systems include yeast, such as Saccharomyces cerevisiae, insect cells such as Spodoptera frugiperda, chicken cells such as E3C/O and SL- 29, mammalian cells such as HeLa, Chinese hamster ovary cells (CHO), COS-7 or MDCK cells, and the like. The foregoing list is illustrative only and is not intended in any way to limit the types of host cells suitable for expression of the nucleic acid sequences of the invention. As used herein, expression vectors refer to any type of vector that can be manipulated to contain a nucleic acid sequence coding for recombinant a-1-antichymotrypsin, such as plasmid expression vectors and viral vectors. The selection of the expression vector is based on compatibility with the desired host cell such that expression of the nucleic acid coding for recombinant a-1antichymotrypsin results. Plasmid expression vectors comprise a nucleic acid sequence of the invention operably <Desc/Clms Page number 11> linked with at least one expression control element such as a promoter. In general, plasmid vectors contain replicon and control sequences derived from species compatible with the host cell. To facilitate selection of plasmids containing nucleic acid sequences of the invention, plasmid vectors may also contain a selectable marker such as a gene coding for antibiotic resistance. Suitable examples include, and are not limited to, the genes coding for ampicillin, tetracycline, chloramphenicol or kanamycin resistance. Suitable expression vectors, promoters, enhancers and other expression control elements are known in the art and may be found in Sambrook et al., Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989). The plasmids and vectors set forth below are examples only and are not meant to be limiting in any way. Plasmids such as pBR322, pUC18, pUC19, pZMS, and pZM may be used for expression in E. coli. Plasmid YRp7 may be used for expression in S. cervisiae. Plasmids such as pMT2 and pMSG may be used for expression in mammals. Suitable viral vectors include baculovirus, Vaccinia virus and adenovirus. The particulars for the construction of expression systems suitable for desired hosts are known to those in the art. For recombinant production of the protein, the DNA encoding it is suitably ligated into the expression system of choice and the system is then transformed into the compatible host cell which is then cultured and maintained under conditions wherein expression of the foreign gene takes place. The peptide of this invention is then recovered from the culture, either by lysing the cells or from the culture medium as appropriate and known to those in the art. Specifically, the expression vector comprising the nucleic acid sequence coding for human a-1antichymotrypsin preferably further comprises transcription and translation control elements operatively linked to the <Desc/Clms Page number 12> nucleic acid sequence coding for human a-1antichymotrypsin. For example, in an upstream position may be a promoter followed by a translation initiation signal comprising a ribosome binding site and an initiation codon, and in a downstream position may be a transcription termination signal. The transcription and translation control elements may be ligated in any functional combination or order. The transcription and translation control elements used in any particular embodiment of the invention will be chosen with reference to the type of cell into which the expression vector will be introduced, so that an expression system is created. It is preferable to use a strong promoter, such as the E. coli trp-lac promoter or the T7 pL promoter, to ensure high levels of expression of the protein product. The plNomp and -lactamase promoters have been found to give low or no yields of a-1-antichymotrypsin when operatively linked with DNA coding for a-1antichymotrypsin. It is also preferable that the promoter is an inducible promoter, such as pL promoter, to avoid possible host cell toxicity during accumulation of the product. Alternatively, a gene expression system based on bacteriophage T7 RNA polymerase as disclosed in Studier and Moffatt, J. Mol. Biol., 1986, 189, 113, which is specifically incorporated as if fully set forth herein, may be used. In this system, E. coli cells transformed with plasmids containing the bacteriophage T7 promoter operatively linked with a DNA sequence coding for a selected product are infected with lambda phage having an expressible gene for T7 RNA polymerase. The cells are infected with phage after sufficient copies of the plasmids are present in the host cells and protein synthesis begins soon after infection. Transformed host cells containing a DNA sequence coding for human a-1-antichymotrypsin analogues may then be grown in an appropriate medium for the host. Where an <Desc/Clms Page number 13> inducible promoter is employed, the host cell may be grown to high density and the promoter turned on for expression of the fusion protein and protease. Where the promoter is not inducible, then constitutive production of the protein product will occur. Constitutive production of the a-1antichymotrypsin analogue is preferable in expressions systems where it is not substantially toxic to the host cell. The cells may be grown until there is no further increase in analogue formation or the ratio of nutrient consumption to analogue formation falls below a predetermined level, at which time the cells may be harvested, lysed and the protein product obtained and substantially purified in accordance with conventional techniques. Such techniques include chromatography, electrophoresis, extraction, and density gradient centrifugation. As used herein, host cells transformed with an appropriate expression vector, and cell cultures of such host cells may be used to synthesize a-1-antichymotrypsin of the present invention. Protein preparations of recombinant analogues of a-1-antichymotrypsin may also be prepared from host cells and cell cultures. Host cells are cultured in medium appropriate to maintain the cells and produce a mixture of cells and medium containing recombinant a-1-antichymotrypsin analogues. Alternatively, the mixture may be purified such that recombinant a-1-antichymotrypsin is purified theref rom. Purification methods useful herein include, but are not limited to, ion exchange chromatography, affinity chromatography, electrophoresis, dialysis and other methods of protein purification known in the art. Protein preparations, of purified or unpurified recombinant a-1antichymotrypsin analogue produced by host cells, are produced which comprise a-1-antichymotrypsin and perhaps other material from the mixture of cells and medium, depending on the degree of purification of the protein. <Desc/Clms Page number 14> Thus, the present invention provides a method of producing human a-1-antichymotrypsin or analogue comprising culturing a host cell capable of expressing human a-1antichymotrypsin or analogue to produce cells containing human a-1-antichymotrypsin or analogue and optionally purifying the mixture to produce human a-1-antichymotrypsin or analogue in a purified form. Protein preparations, of purified or unpurified recombinant a-1-antichymotrypsin analogue produced by host cells, are accordingly produced which comprise a-1antichymotrypsin and other material such as host cell components and/or cell medium, depending on the degree of purification of the protein. The term "purified", when used to describe the state of nucleic acid sequences of the invention, refers to nucleic acid sequences substantially free of nucleic acid not coding for human a-1-antichymotrypsin or other materials normally associated with nucleic acid in non-recombinant cells, i.e., in its "native state". The term "purified" or "in purified form" when used to describe the state of a-1-antichymotrypsin protein or analogue protein, refers to a-1-antichymotrypsin or analogue free, to at least some degree, of cellular material or other material normally associated with it in its native state. Preferably a-1-antichymotrypsin or analogue has a purity (homogeneity) of at least about 25% to about 100%. More preferably the purity is at least about 50%. The analogues of a-1-antichymotrypsin of the present invention exhibit antichymotrypsin activity and are useful in the same manner as native or wild-type a-1antichymotrypsin. The analogues are useful as chymase inhibitors, and in the treatment and prevention of blood clots, reperfusion injury, and inflammation of the lungs. In the case of lung inflammation, inflammation may be caused by aspiration of an acidic substance such as and not limited to stomach contents, smoke, infection, such as <Desc/Clms Page number 15> pathogen infection including infection from a gram negative bacterium (Escherichia and Pseudomonas, for example). The analogues of the invention may be administered with a pharmaceutically-acceptable carrier or diluent, such as a saline solution or other buffer. Suitable pharmaceutical carriers are well known in the art and are described for example, in Gennaro, Alfonso, ed., Remington's Pharmaceutical Sciences, 18th Edition, 1990, Mack Publishing Co. , Easton PA, a standard reference text in this field. Carriers may be selected with regard to the intended route of administration and the standard pharmaceutical practice. Dosages will be set with regard to weight, and clinical condition of the patient. The proportional ratio of active ingredient to carrier will naturally depend on the chemical nature, solubility, and stability of the analogues, as well as the dosage contemplated. The analogues of the invention may be employed in the method of the invention singly or in combination with other compounds, including and not limited to other analogues of the invention. The method of the invention may also be used in conjunction with other treatments including and not limited to antibodies, toxins, and antisense oligonucleotides. For in vivo applications the amount to be administered will also depend on such factors as the age, weight, and clinical condition of the patient. The analogues of the present invention may be administered by any suitable route, including inoculation and injection, for example, intravenous, oral, intraperitoneal, intramuscular, subcutaneous, topically, and by absorption through epithelial or mucocutaneous linings, for example, nasal, oral, vaginal, rectal and gastrointestinal. The mode of administration of the analogues, medicaments, and compositions of the present invention, may determine the sites in the organism to which the analogue may be delivered. For instance, topical application may be administered in creams, ointments, gels, oils, emulsions, <Desc/Clms Page number 16> pastes, lotions, and the like. For parenteral administration, the analogues may be used in the form of a sterile aqueous solution which may contain other solute, for example, sufficient salts, glucose or dextrose to make the solution isotonic. For oral mode of administration, the present invention may be used in the form of tablets, capsules, lozenges, troches, powders, syrups, elixirs, aqueous solutions and suspension, and the like. Various disintegrants such as starch, and lubricating agents may be used. For oral administration in capsule form, useful diluents are lactose and high molecular weight polyethylene glycols. When aqueous suspensions are required for oral use, certain sweetening and/or flavoring agents may be added. Methods of inhibiting chymase, treating skin inflammation, treating and preventing blood clotting, treating lung inflammation, and treating reperfusion injury are also provided whereby an a-1-antichymotrypsin analogue having amino acid substitutions at position 358, or positions 356-361 with position 358 selected from the amino acids identified above; or wherein the amino acids of human wild type a-1-antichymotrypsin Thr-Leu-Leu-Ser-Ala-Leu corresponding to positions 356 - 361 are substituted with Ile-Pro-Xxx-Ser-Ile-Pro; is added to a sample exhibiting the condition to be treated. For purposes of the current invention, animals include but are not limited to the Order Rodentia, such as mice and the Order Logomorpha, such as rabbits; more particularly the Order Carnivora, including Felines (cats) and Canines (dogs); even more particularly the Order Artiodactyla, Bovines (cows) and Suines (pigs) and the Order Perissodactyla, including Equines (horses); and most particularly the Order Primates, Ceboids and Simoids (monkeys) and Anthropoids (humans and apes). The animals of the most preferred embodiments are humans. <Desc/Clms Page number 17> EXAMPLES PRODUCTION AND PURIFICATION OF RECOMBINANT &alpha;-1- ANTICHYMOTRYPSIN ANALOGUES Materials Chymotrypsin was obtained from Sigma or Boehringer-Mannheim. All chromophoric protease substrates were obtained from Bachem, as was phenylmethylsulfonyl fluoride (PMSF). Human serum a-1-antichymotrypsin was prepared using a procedure based on the work of Tsuda et al., Tokai, J. Exp. Clin. Med. , 1982,7, 201. This method affords pure a-1-antichymotrypsin in three steps, batchwise elution from DNA cellulose, G-150 chromatography and KC1 gradient elution from DNA cellulose. Plasmid constructions and DNA manipulations were carried out following Sambrook et al., Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989). Identification and Sequencing of the Gene from Human Antichymotrypsin A human liver cDNA library in the phage expression vector lambda-gtil provided by Mitchell Weiss, Department of Human Genetics, University of Pennsylvania, was screened according to the method of Young and Davis, Proc. Natl. Acad. Sci., U.S.A., 1977,74, 5463, with polyclonal antisera raised against Cl esterase inhibitor (DAKO, Santa Barbara, CA), a related serine protease inhibitor. Positives clones were picked, rescreened and plaque-purified. DNA sequencing was performed with the chain termination method of Sanger et al., Proc. Natl. Acad. Sci., 1977,74, 5463, using oligonucleotide primers obtained from the Nucleic Acid Synthesis Center of the Wistar Institute (Philadelphia, PA). The DNA sequence and the derived amino acid sequence of the EcoR1 fragment from one of the positive lambda-gtll cDNA clones contained the entire coding region of the mature human a-1-antichymotrypsin, as depicted in <Desc/Clms Page number 18> Figure 1. The construct also included a 21 nucleotide extension of the 5'-end encoding 7 amino acids, comprising the sequence 5'-Met-Ala-Ser-Leu-Cys-His-Pro- (SEQ ID NO: 5). The mature protein contains 398 amino acids (Mr 45,031) starting from amino acid position 1, Asn, at the amino terminus and contains a single cysteine residue at position 236. Preparation of Recombinant &alpha;-1-Antichymotrypsin and Analogues Having Tryptophan at Amino Acid Position 358 The nucleic acid sequence coding for human a-1antichymotrypsin may be obtained as described above and subcloned into pUC19 (Promega, Madison, Wis. ) as set forth in U.S. Patent 5,079,336, incorporated herein by reference. In the present example, however, the analogue of a-1antichymotrypsin having the substitution of tryptophan for leucine at position 358, rACT-L358W, was prepared according to PCR as follows. The commercially available plasmid pKC30 was cut by BamHI, the single strand created staggered ends which were filled by Klenow reaction. The linearized plasmid with both ends blunt ended was self ligated, and E. coli N4830-1 was transformed by the ligation reaction mixture. The plasmid purified from transformants was pKC30(-BamHI), indicating pKC30 with the only BamHI site removed. This pKC30 (-BamHI) was digested by HpaI, and created two blunt ends for the next ligation step. A 0.2 kb fragment containing a ribosome binding site was cut out of the following set of plasmids: pAR 3038, pAR3039, and pAR3040, by XbaI and EcoRV. The EcoRV cut created a blunt end. The staggered end created by XbaI was filled by the Klenow reaction. With both ends blunted, the three 0.2 kb fragments were separately ligated to HpaI digested pKC30(-BamHI). The three ligation mixtures were used to transform N4830-1 separately. Three vectors were obtained from these transformants. They were named pZM3038, pZM3039, and pZM3040, corresponding to three reading frames. These pZM vectors contained a pL promoter <Desc/Clms Page number 19> from pKC30, and received Shine-Dalgarno sequences from pAR vectors. The unique cloning site of these vectors is BamHI which is located in the fragment from pAR. One of pZM3038, pZM3039, or pZM3040 was cut by NheI, and produced two fragments, about 5.9 kb, and about 0.75 kb. The 5.9 kb fragment was gel purified and ligated to recircularize. N4830-1 was transformed with this , ligation reaction. The plasmid isolated from the transformants was named pZMs. pZMs has a unique cloning site - NheI which is downstream from the pL promoter, Shine-Dalgarno sequence, and the start codon. Primers 1, 2,3, and 4, as shown in Table 1, were prepared by standard techniques set forth in Sambrook et al., Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989) and used in a polymerase chain reaction (PCR) protocol to introduce bases coding for tryptophan into the sequence coding for a-1-antichymotrypsin. Standard PCR protocols are known to those skilled in the art. Table 1 EMI19.1 <tb> <tb> Primer <SEP> Sequence <SEP> SEQ <SEP> ID <SEP> NO: <tb> 1 <SEP> 5'-CCCCATATGGCTAGCAACAGCCCACTTG-3' <SEP> 1 <tb> 2 <SEP> 5'-TAATGCAGACCAGAGGGTGA-3' <SEP> 2 <tb> 3 <SEP> 5'-ATCACCCTCTGGTCTGCATTA-3' <SEP> 3 <tb> 4 <SEP> 5'-TTTCATATGGCTAGCGCTCTAGGCTTGC-3' <SEP> 4 <tb> The full-length nucleotide sequence was created in two parts, Fragments A and B, which were combined to produce the full-length nucleotide sequence. To construct Fragment A, two primers were used. Primer 1 contains the 5' sequence of the coding or sense strand for a-1-antichymotrypsin with an extended 5' tail which contains an NheI cloning site, which is shown underlined in Table 1. Primer 2 comprises a 3' tail having bases coding for tryptophan to replace the bases coding for <Desc/Clms Page number 20> leucine at position 358 (the tryptophan codon is shown in bold letters in Table 1). Primers 1 and 2 were then used with the polymerase chain reaction and the sequence coding for a-1-antichymotrypsin subcloned in pUC19 to produce Fragment A that codes for the N-terminus through position 358. Standard PCR methods were followed for the production of Fragment A. 1 l of 100 ng/1 of Primer 1 and 1 l of 100 ng/1 of Primer 2,10 l of lOx PCR buffer, 10 l of 2mM dNTP and 0.5 l of Taq enzyme were added to 10 ng of template DNA, pUC19 containing the a-1antichymotrypsin gene, with distilled water bringing the reaction volume to 100 l. Three steps of PCR were performed, 94 C for 15 seconds, 52 C for 15 seconds, and 72 C for 1 second. The three steps equal one cycle, thirty cycles were run. To construct Fragment B, two different primers were used. Primer 3 contains nucleotides of the coding or sense strand comprising a 3' tail having bases coding for tryptophan to replace the bases coding for leucine at position 358 (the tryptophan codon is shown in bold letters in Table 1). Primer 4 has an extended 5' tail that contains an NheI cloning site, which is shown underlined in Table 1. Primers 3 and 4 were then used with the polymerase chain reaction and the sequence coding for a-1antichymotrypsin subcloned in pUC19 to produce fragment B that codes for position 358 through the C-terminus. The PCR protocol set forth above for the production of Fragment A was also followed for the production of Fragment B. The full-length sequence was then produced by the protocol set forth above for Fragment A, with 5-10 ng of each of Fragment A and Fragment B. The fragments were denatured and reannealed. The fragments were reannealed to produce heteroduplexes of Fragments A and B overlapping at a sequence coding for tryptophan that was created via Primers 2 and 3. The heteroduplexes of Fragments A and B were extended using Taq DNA polymerase with the overlapping <Desc/Clms Page number 21> portions of Fragments A and B serving as primers to produce the full-length sequence coding for a-1-antichymotrypsin having tryptophan substituted for leucine at position 358 of the wild-type. The full-length sequences were then amplified using Primers 1 and 4. The amplified full-length sequence was then digested with NheI and inserted into the expression vector pZMs, which was digested with NheI. E. coli was then transformed with pZMs containing the full length gene. As such, analogues of a-1-antichymotrypsin with an altered amino acid position 358 of the reactive site were expressed. Thus, leucine at amino acid position 358 was changed to tryptophan. This analogue is represented herein as rACT-L358W. Other analogues of human wild type a-1antichymotrypsin of the present invention may be similarly prepared. Preparation of Recombinant a-1-Antichymotrypsin Having MetAla-Ser or Ala-Ser N-Terminal Extension Stable monomers were expressed directly by eliminating the N-terminal cysteine residue through deletion of the nucleotides encoding the Leu-Cys-His-Pro (SEQ ID NO: 6) sequence within the N-terminal sequence MetAla-Ser-Leu-Cys-His-Pro (SEQ ID NO: 5) encoded within the nucleotide sequence of wild type a-1-antichymotrypsin to produce the nucleotide sequence encoding rMAS-ACT. As such, the amino acids leucine, cysteine, histidine and proline at amino acid positions-4 to-1 of wild type a-1antichymotrypsin were deleted. The N-terminal extension becomes methionine-alanine-serine at amino acid positions -3 to -1. Such extension was accomplished by PCR amplification, set forth above, of the entire coding sequence with an N-terminal primer 5' -CCCCATATGGCTAGCAACAGCCCACTTG-3' (SEQ ID NO: 1). The Cterminal primer was 5'-TTTCATATGGCTAGCGCTCTAGGCTTGC-3' (SEQ ID NO: 4) in which the underlined sequence GCTAGC served as the insertion site. The underlined CTA sequence creates a TAG termination codon in the sense strand of the vector. <Desc/Clms Page number 22> The sequence was cloned into pZMs. The amplified fulllength sequence was digested with NheI and inserted into the expression vector pZMs, which was digested with Nhe I. E. coli was then transformed with pZMs containing the gene with the altered N-terminal extension. As such, analogues of a-1-antichymotrypsin were produced by altering amino acid positions-4 to -1, such that the amino acids leucine, cysteine, histidine and proline were deleted. This analogue is represented herein as rMAS-ACT. Expression of rACT Analogues E. coli N4830-1 was transformed with the expression vectors pACT-L358W, pMAS-ACT, pAS-ACT, pMAS-ACTL358W or pAS-ACT-L358W by standard calcium chloride methods as described by Sambrook et al., Molecular Cloning: A Laboratory Manual, second edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989). Small scale growth conditions and extraction Fresh overnight cultures of E. coli strain N4830- 1 transformed with the aforementioned expression vectors were diluted to 1.5% in LB broth containing ampicillin (Na+ salt, 0.1 mg/ml) and grown at 30 C in a shaking incubator to an A600nm of 0.18, induced by raising the temperature to 42 C and grown for an additional five to eight hours. The cells were centrifuged and disrupted in a French press. Purification and Characterization of Recombinant a-1-Antichymotrypsin Analogues Large-Scale Growth of E. coli E. coli strain N4830-1 transformed with the aforementioned expression vectors were grown in LB media containing ampicillin (Na+ salt, 0.1 m/ml) at 30 C to an A600nm of 0.18 in a 15 L carboy fitted with an oxygen bubbler. The cells were induced by raising the temperature to 42 C and grown for an additional six hours to a final A600nm of approximately 0.9 - 1.0. <Desc/Clms Page number 23> Extraction and column chromatography All purification steps were carried out at 4 C. In a typical preparation of a-1-antichymotrypsin analogue, cell paste was dispensed in 10 mM potassium phosphate buffer, pH 6.9 (25 ml) and lysed by three passes through a French press at 10,000 psi and 4 C. Cell debris was removed by centrifugation at 30,000 x g for 30 minutes at 4 C. Supernatant (25 ml) was loaded onto a column (4.9 cm2 x 37 cm) of Sepharose Fast Q (Pharmacia) that had been equilibrated to 50 mM Tris-Cl, pH 7.5, containing 50 mM KCl. Protein eluted with a linear gradient of KCl in 50 mM Tris-Cl, pH 7.5 (50-500 mM in 2 L). Fractions (15 ml) were monitored for protein by A280nm and assayed for antichymotrypsin activity as described herein. Analogue eluted at approximately 200 mM KC1. Fractions containing analogue were combined and dialyzed against two volumes (2.5 L each) of 10 mM potassium phosphate buffer, pH 6.9 over 48 hours. The dialyzed solution was then applied to a DNA-cellulose column (1.7 cm2 x 20 cm) that had been preequilibrated with 10 mM potassium phosphate, pH 6.9, containing 10 mM KC1. After loading, the column was first washed with the same buffer (20 ml). The column was eluted with a linear gradient of KC1 (10-500 mM, 300 ml) in the same buffer. Fractions (8 ml) were assayed for protein and antichymotrypsin activity as described herein. Analogue eluted at approximately 350 mM KC1. Fractions containing antichymotrypsin activity were analyzed for purity by SDSPAGE, performed according to Laemmli, Nature (London) 1970, 227,680. Each portion was concentrated by ultrafiltration using Amicon YM-10 membranes and dialyzed overnight against 50 mM Tris-Cl, pH 7.5 (500 ml). In some cases recombinant protein was further purified on an FPLC Mono Q anion exchange column, using the conditions described above. Antichymotrypsin Activity Assays Antichymotrypsin activity could not be directly measured in crude bacterial lysates because of large <Desc/Clms Page number 24> background inhibitory activity in the lysate itself. The background activity was separated from the antichymotrypsin by anion exchange chromatography using a Mono Q HR5/5 anion exchange FPLC column (Pharmacia) fitted into a pump (LKB 2150 pump), a 2152 gradient controller and a UV absorbance detector (Waters 440 UV absorbance detector) with an extended wavelength module. Chromatography was typically conducted on the extract from 200 mg of cells. The separation involved an isocratic wash (5 minutes) with 50 mM Tris-Cl buffer, pH 7.5, containing 50 mM KC1, followed by a linear gradient of KC1 (50-350 mM in 30 minutes) at a flow rate of 1.0 ml/minute. Protein absorbance was monitored at both 214 and 280 nm. Fractions (1.0 ml) were collected and assayed for antichymotrypsin or antitrypsin activity measured as the inhibition of the chymotrypsincatalyzed hydrolysis of substrate N-suc-Ala-Ala-Pro-Phe-pnitroanilide (0.1 ml of 10 mM solution in 90% DMSO), DelMar et al., Anal. Biochem., 1979, 99,316 or of trypsincatalyzed hydrolysis of substrate N-Bzl-Pro-Phe-Arg-pnitroanilide (0.02 ml of a 14.6 mM solution in 90% DMSO). A typical chymotrypsin assay contained in (1.0 ml): 100 mM Tris-Cl buffer, pH 8.3, 0.005% (v/v) Triton X-100, bovine pancreatic chymotrypsin (18 mmol) and column eluate (0.005 - 0.5 ml). The assay mixture was pre-incubated at room temperature for 5 minutes, substrate (0.01 ml of a 10 mM solution in 90% DMSO) was added, and remaining chymotrypsin activity was determined by the rate of change in A410nm caused by the release of p-nitroaniline. A typical trypsin assay contained (in 1.0 ml); 100 mM Tris-Cl buffer, pH 8.3,0.005% (v/v) Triton X-100, bovine trypsin (8.6 pmol) and sample (0.005 - 0.5 ml). The assay mixture was preincubated at room temperature for 10 minutes before substrate (0.02 ml of a 15 mM solution in 90% DMSO) was added and remaining trypsin activity was determined as above. Measurements of optical absorbance were conducted at 25 C using a spectrophotometer (Hewlett Packard 8452A) fitted with a temperature controlled sample compartment. <Desc/Clms Page number 25> INTERACTION OF RECOMBINANT a-1-ANTICHYMOTRYPSIN AND ANALOGUES WITH HUMAN CHYMASE Materials Peptide-nitroanilide substrates were purchased from Sigma or Bachem. TSK-Heparin-5PW HPLC column was purchased from Supelco. Heparin-Sepharose was from Pharmacia. Immobilon-P (PVDF membranes) was purchased from Millipore. The analogue of antichymotrypsin wherein the leucine at position 358 of wild-type is substituted with tryptophan (W) was produced as described herein. Purification of Human Chymase Mast cell proteinases were extracted from human skin and fractionated on a 500 ml heparin-Sepharose column. Chymase, eluted from heparin-Sepharose in single step with 2 M NaCl, 0.1 M MOPS (pH 6.8) was further purified by affinity chromatography using soybean trypsin inhibitor (SBTI) -Sepharose. The fraction of chymase binding to heparin-Sepharose was variable. In extracts resulting in an enzyme heparin-Sepharose binding of 30-70, an alternate purification method was used. Chymase in the flow-through was precipitated by addition of protamine chloride. After solubilization of the precipitate in high salt buffer, chymase was absorbed to a phenylbutylamine-Sepharose column (20 ml resin) and chymase was eluted in a single step by addition of 0.4 M NaCl, 0.01 M [3-(N-morpholino)-propanesulfonic acid], MOPS, (pH 6.8), 0.2 M D tryptophan methyl ester, D-Trp-OMe, solution. The proteinase was then applied to a heparin-Sepharose column where it absorbed to the column and was eluted with a salt gradient. Chymase purified by both procedures had identical catalytic properties, migrated identically on SDS gels, and had the same stoichiometry of inhibition when titrated against serum ACT. Enzyme purified by both procedures was used in these studies. Determination of Inhibitor and Proteinase Concentrations <Desc/Clms Page number 26> Concentrations of a-1-recombinant antichymotrypsins were determined by titration with chymotrypsin. Titrations were performed in 50 l (25 C) containing in 0.1 M Tris-HCl, 0.5 M NaCl, 0.01% Triton X-100 and 150-300 M proteinase. Residual activity was determined after 10-15 minute incubations by diluting sample with 1 ml assay buffer containing the substrates SucAAPF-pNA or CBZ-GPA-pNA (carbobenzoxy-Gly-Pro-Arg pnitroanilide) at 1 mM. Stock chymotrypsin solution was standardized with the active site titrants N-trans-cinnamoylimidazole. Chymase concentrations were determined using its specific activity 2.7 mol of product min-1/nmol chymase measured under standard assay conditions containing 1 mM of substrate SucAAPF-pNA; #410 = 8800 (the extinction coefficient of p-nitroaniline) was used to quantify released p-nitroanaline. Chymase Assay Reactions of chymase with recombinant a-1antichymotrypsin and analogues were performed at 25 C in 50-150 l of a solution containing 0.2 M Tris-HCl (pH 8.0), 1M NaCl, 0.01% Triton X-100 and 150-400 nM chymase. After incubation with inhibitor, independently including the aforementioned analogues, for appropriate times, residual activity was measured by dilution of a sample aliquot in 1 ml assay buffer containing 1 mM substrate as described above. The reactions of chymase with inhibitor were stopped by the addition of a general serine proteinase inhibitor (PMSF) and a sample of the reaction mixture was electrophoresed to determine reaction products. Reaction of Chymase with Variant rACTs Stoichiometry of inhibition (SI) values and second order rate constants were determined for the analogues. Titrations (pH 8.0,1 M NaCl, 25 C) to obtain the SI for each recombinant a-1-antichymotrypsin are presented in Figure 2. Residual activities in these <Desc/Clms Page number 27> studies were achieved within minutes and remained constant for at least 24 hours. Second order rate constants (kobs/I) were evaluated for all rACTS at (rACT)o/(chymase)o 1. The reaction of chymase with serum a-1-antichymotrypsin, reactions appears pseudo first order when (I)o/(E)o ratios are approximately 10 fold higher than the SI. Reaction conditions in rate studies were similar to titrations, measurements were made at NaCl concentrations of 1 M and 2 M. Inhibition Rate Constants Progress curves were determined under pseudo-first order conditions ((I)o (E)) in the presence of substrate. Reactions contained 0.25 to 1.0 mM SucAAPF-pNA, 2-2.5 nM chymase and inhibitor. Absorbance was continually monitored for 15 minutes, and instantaneous velocities were determined over 1 minute intervals by regression analysis. Plots of the instantaneous velocity vs time (t) were fit by non-linear methods to the expression Ae-k't to determine k', the apparent first order rate constant; (A) is the initial activity. kobs/I was calculated from the relationship, kobs/I = k'((S/Km)+1)/I, where S is the initial substrate concentration, Km is the Michaelis constant for chymase hydrolysis of the substrate under the experimental conditions, and I is the initial inhibitor concentration. Reactions were monitored for at least three half-lives. Km was 0.80 mM in 1.0 M NaCl and 0.49 in 2.0 M NaCl. Rate constants and SI for all variants are collected in Table 2. Only minor changes in values were observed at the two different NaCl concentrations. The results illustrate the importance of the P1 residue, change of Leu to Trp, in modulating the SI and rate of inhibition. The reaction of chymase with serum ACT has a stoichiometry of inhibition (SI) of 4.0 due to generation of degraded inhibitor (3.5 moles degraded inhibitor/mole chymase) in a reaction competing with chymase inhibition. <Desc/Clms Page number 28> Table 2 Kinetic Parameters for the Reaction of Human Chymase with Recombinant a-1-Antichymotrypsin and Analogues EMI28.1 <tb> <tb> rACT <SEP> NaCl <SEP> kobs/Ia <SEP> SI <tb> variant <SEP> (M) <SEP> (M-1s-1) <tb> Leu358W <SEP> 1 <SEP> 151,000 <SEP> 1.5 <tb> Leu358W <SEP> 2 <SEP> 280,000 <SEP> 1.2 <tb> Leu358 <SEP> 1 <SEP> 27,000 <SEP> 4.0 <tb> Leu358 <SEP> 2 <SEP> 42,500 <SEP> 3.5 <tb> Leu358(Cas) <SEP> 1 <SEP> 60,000 <SEP> 2.0 <tb> a - experimental conditions for measurement rate constants were similar to those of titrations except for presence of substrate and 9% Me2S04. Most kobs/[I] values are the average of two experiments and deviation from the average was typically less than 15%. Kobs/[I] values for rACT-L358 in 2 M NaCl and rACT-L258W in 1 M NaCl were based on 4 and 5 measurements, respectively; SD were 8% and 15% of the reported values respectively. Leu358(Cas) refers to the cassette version of the analogue prepared according to the methods set forth in U.S. Patent 5,079,336. Various modifications of the invention in addition to those shown and described herein will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. <Desc/Clms Page number 29> SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Cooperman, Barry; Rubin, Harvey; Schecter, Norman; Wang, Zhi Mei (ii) TITLE OF INVENTION: a-1-Antichymotrypsin Having Chymase Inhibiting Activity (iii) NUMBER OF SEQUENCES: 8 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: Woodcock Washburn Kurtz Mackiewicz & Norris (B) STREET: One Liberty Place - 46th Floor (C) CITY: Philadelphia (D) STATE: PA (E) COUNTRY: USA (F) ZIP: 19103 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: DISKETTE, 3.5 INCH, 1.44 Mb STORAGE (B) COMPUTER: IBM PS/2 (C) OPERATING SYSTEM: PC-DOS (D) SOFTWARE: WORDPERFECT 5.1 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: Herewith (C) CLASSIFICATION: (vii) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: (viii) ATTORNEY/AGENT INFORMATION: <Desc/Clms Page number 30> (A) NAME: Lori Y. Beardell (B) REGISTRATION NUMBER: 34,293 (C) REFERENCE/DOCKET NUMBER: UPN-0963 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (215) 568-3100 (B) TELEFAX: (215) 568-3439 (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: CCCCATATGG CTAGCAACAG CCCACTTG (3) INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 .base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: TAATGCAGAC CAGAGGGTGA (4) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear <Desc/Clms Page number 31> (ii) MOLECULE TYPE: cDNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: ATCACCCTCT GGTCTGCATT A (5) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: TTTCATATGG CTAGCGCTCT AGGCTTGC (6) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acids (B) TYPE: protein (C) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: Met-Ala-Ser-Leu-Cys-His-Pro (7) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acids (B) TYPE: protein (C) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: Leu-Cys-His-Pro <Desc/Clms Page number 32> (8) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: protein (C) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: Thr-Leu-Leu-Ser-Ala-Leu (9) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: protein (C) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: Ile-Pro-Xxx-Ser-Ile-Pro

Claims: WHAT IS CLAIMED: 1. An analogue of human wild type a-1antichymotrypsin where--. the amino acid corresponding to the leucine at amino acid position 358 of wild-type a-1antichymotrypsin is substituted with an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 2. An analogue of human wild type a-1antichymotrypsin wherein the amino acids corresponding to the amino acids Thr-Leu-Leu-Ser-Ala-Leu at positions 356 to 361 of wild-type a-1-antichymotrypsin are substituted with amino acids Ile-Pro-Xxx-Ser-Ile-Pro, wherein Xxx is selected from the group consisting of methionine, tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 3. An analogue of claim 2 wherein the amino acid at Xxx is methionine. 4. An analogue of claims 1,2, or 3 having chymase inhibiting activity. 5. An analogue of claim 1 comprising the amino acid sequence set forth in Figure 1 wherein amino acid position 358 is substituted with an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. <Desc/Clms Page number 34> 6. The analogue of claim 1 comprising the amino acid sequence set forth in Figure 1 with leucine at amino acid position 358 substituted with tryptophan. 7. The analogue of claim 2 comprising the sequence set forth in Figure 1 wherein amino acids Thr-LeuLeu-Ser-Ala-Leu at positions 356 to 361 are substituted with amino acids Ile-Pro-Xxx-Ser-Ile-Pro, wherein Xxx is selected from the group consisting of methionine, tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 8. An analogue of claims 1,2, or 3 optionally comprising an N-terminal extension selected from the group consisting of methionine-alanine-serine and alanine-serine. 9. An analogue of claim 3 comprising the nucleotide sequence set forth in Figure 1 wherein amino acid positions 356-361 are Ile-Pro-Xxx-Ser-Ile-Pro and wherein amino acid position is methionine. 10. An analogue of claim 1 comprising the nucleotide sequence set forth in Figure 1 wherein the nucleotides coding for with leucine at amino acid position 358 substituted with the nucleotides coding for tryptophan. 11. The analogue of claim 2 comprising the nucleotide sequence set forth in Figure 1 wherein nucleotides coding for amino acids Thr-Leu-Leu-Ser-Ala-Leu at positions 356 to 361 are substituted with nucleotides coding for amino acids Ile-Pro-Xxx-Ser-Ile-Pro, wherein Xxx is selected from the group consisting of methionine, tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, <Desc/Clms Page number 35> lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 12. The analogue of claim 11 wherein the amino acid Xxx is methionine. 13. A purified nucleic acid sequence coding for an analogue of human wild type a-1-antichymotrypsin wherein the amino acid corresponding to the leucine at amino acid position 358 of human wild-type a-1-antichymotrypsin is substituted with an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 14. A purified nucleic acid sequence of an analogue of human wild type a-1-antichymotrypsin wherein nucleic acids coding the amino acids corresponding to the amino acids Thr-Leu-Leu-Ser-Ala-Leu at positions 356 to 361 of wild-type a-1-antichymotrypsin are substituted with nucleic acids coding for amino acids Ile-Pro-Xxx-Ser-IlePro, wherein Xxx is selected from the group consisting of methionine, tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 15. The purified nucleic acid sequence of claim 14 wherein the amino acid Xxx is methionine. 16. The purified nucleic acid sequence of claims 13,14, or 15 optionally having an N-terminal extension selected from the group consisting of methionine-serinealanine and alanine-serine. <Desc/Clms Page number 36> 17. The nucleic acid sequence of claim 1 wherein said human wild type a-1-antichymotrypsin nucleic acid sequence comprises the sequence set forth in Figure 1. 18. An analogue of human wild type a-1antichymotrypsin wherein the analogue has a sequence substantially identical to human wild type a-1antichymotrypsin and an N-terminal extension selected from the group consisting of methionine, alanine and serine; and alanine and serine. 19. The analogue of human wild type a-1antichymotrypsin of claim 15 wherein human wild type a-1antichymotrypsin has the amino acid sequence set forth in Figure 1. 20. A purified nucleic acid sequence coding for an analogue of human wild type a-1-antichymotrypsin wherein the analogue has a sequence substantially identical to wild type a-1-antichymotrypsin and an N-terminal extension selected from the group consisting of methionine, alanine and serine; and alanine and serine. 21. The nucleic acid sequence of claim 20 wherein said nucleic acid sequence of human wild type a-1antichymotrypsin comprises the sequence set forth in Figure 1. 22. An expression vector comprising the nucleic acid sequence of the analogue of claim 1. 23. An expression vector comprising the nucleic acid sequence of the analogue of claim 2. 24. An expression vector comprising the nucleic acid sequence of the analogue of claim 3. <Desc/Clms Page number 37> 25. An expression vector comprising the nucleic acid sequence of the analogue of claim 5. 26. A host cell capable of expressing the nucleic acid sequence of the analogue of claim 1. 27. A host cell capable of expressing the nucleic acid sequence of the analogue of claim 2. 28. A host cell capable of expressing the nucleic acid sequence of the analogue of claim 3. 29. A host cell capable of expressing the nucleic acid sequence of the analogue of claim 5. 30. A cell culture capable of expressing a-1antichymotrypsin wherein the amino acid corresponding to the leucine at amino acid position 358 of wild-type a-1antichymotrypsin is substituted with an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine, obtained by transforming a cell with an expression vector comprising the nucleic acid sequence set forth in Figure 1, wherein said vector further comprises nucleic acids coding for an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine at amino acid position 358. 31. A cell culture capable of expressing a-1antichymotrypsin wherein the amino acid corresponding to Thr-Leu-Leu-Ser-Ala-Leu at positions 356 to 361 of wildtype a-1-antichymotrypsin are substituted with amino acids <Desc/Clms Page number 38> Ile-Pro-Xxx-Ser-Ile-Pro, said a-1-antichymotrypsin obtained by transforming a cell with an expression vector comprising the sequence set forth in Figure 1, wherein said vector further comprises nucleic acids coding for Ile-Pro-Xxx-SerIle-Pro at amino acid positions 356-361, and Xxx is selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 32. A cell culture of claim 31 wherein the amino acid Xxx is methionine. 33. A protein preparation comprising the analogue of human a-1-antichymotrypsin of claim 1. 34. A protein preparation comprising the analogue of human a-1-antichymotrypsin of claim 2. 35. A protein preparation comprising the analogue of human a-1-antichymotrypsin of claim 3. 36. A protein preparation comprising the analogue of human a-1-antichymotrypsin of claim 5. 37. A method of inhibiting chymase comprising contacting said chymase with an effective amount of the analogue of human a-1-antichymotrypsin of claims 1, 2, or 3. 38. A method of inhibiting chymase comprising contacting said chymase with an effective amount of the analogue of human a-1-antichymotrypsin of claim 5. 39. A method of using an analogue of human wild type a-1-antichymotrypsin to inhibit chymase activity <Desc/Clms Page number 39> comprising the step of contacting said chymase with an effective amount of said analogue, said analogue having the amino acid corresponding to the leucine at amino acid position 358 of wild-type a-1-antichymotrypsin substituted with an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 40. A method of using an analogue of human wild type a-1-antichymotrypsin to inhibit chymase activity comprising the step of contacting said chymase with an effective amount of said analogue, said analogue having the amino acids corresponding to the amino acids Thr-Leu-LeuSer-Ala-Leu at positions 356 to 361 of wild-type a-1antichymotrypsin are substituted with amino acids Ile-ProXxx-Ser-Ile-Pro, wherein Xxx is selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 41. A method of claim 40 wherein the amino acid Xxx is methionine. 42. A method of using an analogue of human wild type a-1-antichymotrypsin to inhibit chymase activity comprising the step of contacting said chymase with an effective amount of said analogue, said analogue having the amino acid corresponding to the leucine at amino acid position 358 of wild-type a-1-antichymotrypsin substituted with tryptophan. 43. A composition comprising an effective amount of analogue of human wild type a-1-antichymotrypsin in a pharmaceutically acceptable carrier, said analogue <Desc/Clms Page number 40> having the amino acid corresponding to the leucine at amino acid position 358 of wild-type a-1-antichymotrypsin substituted with an amino acid selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 44. A composition comprising an effective amount of an analogue of human wild type a-1antichymotrypsin and a pharmaceutically acceptable carrier, said analogue having the amino acids corresponding to the amino acids Thr-Leu-Leu-Ser-Ala-Leu at positions 356 to 361 of wild-type a-1-antichymotrypsin are substituted with amino acids Ile-Pro-Xxx-Ser-Ile-Pro, wherein Xxx is selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. 45. The composition of claim 44 wherein the amino acid Xxx is methionine. 46. Use of an analogue of human a-1antichymotrypsin wherein the amino acids corresponding to Thr-Leu-Leu-Ser-Ala-Leu at positions 356 to 361 of wild type a-1-antichymotrypsin are substituted with Ile-Pro-MetSer-Ile-Pro in the preparation of a medicament for the treatment of inflammation of the lung. 47. The use of claim 46, wherein said inflammation results from aspiration of an acidic substance. 48. The use of claim 47, wherein the acidic substance is stomach contents. <Desc/Clms Page number 41> 49. The use of claim 47 wherein the acidic substance is smoke. 50. The use of claim 46 wherein said inflammation results from infection by a pathogen. 51. The use of claim 50 wherein said pathogen is a gram-negative bacterium. 52. Use of an analogue of human a-1antichymotrypsin wherein the amino acid at position 358 is selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, valine, and arginine in the preparation of a medicament for the treatment of reperfusion injury. 53. Use of an analogue of human a-1antichymotrypsin wherein the amino acid at position 358 is selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, valine, and arginine in the preparation of a medicament for the treatment or prevention of blood clots. 54. Use of an analogue of human a-1antichymotrypsin wherein the amino acid at position 358 is selected from the group consisting of tryptophan, alanine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, valine, and arginine in the preparation of a medicament for the treatment of inflammation of the lung. <Desc/Clms Page number 42> 55. The use of any one of claims 52,53, or 54, wherein said amino acid position 358 is arginine. 56. The use of any one of claims 52,53, or 54, wherein said amino acid position 358 is tryptophan. 57. The use of any one of claims 52,53, or 54, wherein said amino acid position 358 is phenylalanine.

Inventors: COOPERMAN BARRY S, RUBIN HARVEY, SCHECHTER NORMAN, WANG ZHI MEI, COOPERMAN, BARRY, S., RUBIN, HARVEY, SCHECHTER, NORMAN, WANG, ZHI, MEI
Applicants: UNIV PENNSYLVANIA, THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
Application year: 1994
Main classification: C12N15
IPC codes: A61K38, A61P7, A61P9, A61P29, A61P31, C07H21, C07K14, C12N1, C12N5, C12N9, C12N15, C12P21, C12R1