TNF-ALPHA MUTEINS AND A PROCESS FOR PREPARING THEM

Abstract: The invention relates to a tumor necrosis factor mutein characterized in that the TNF- alpha amino acid sequence is mutated, or deleted totally or partially, in the region extending from amino acid position at 101 to 116 in such a way that: either the lectin-like activities are modulated with respect to TNF- alpha , and/or the toxic activity is reduced with respect to TNF- alpha , and/or the inflammatory cytokines inducing capacities are modulated with respect to TNF- alpha , and/or the adhesion molecules inducing capacities are modulated with respect to TNF- alpha , and/or the metastasis promoting activity is reduced with respect to TNF- alpha , and/or the half life time is increased with respect to TNF- alpha and with said muteins being characterized in that they have retained the aforementioned activities; or a pharmaceutically acceptable salt thereof.

Description: TNF-ALPHA MUTEINS AND A PROCESS FOR PREPARING THEM. The present invention relates to TNF-α-muteins, a process for preparing them and their use as active substances in pharmaceutical compositions. Tumor necrosis factor-α (TNF-α) , also known as cachectin, is a multifunctional cytokine produced mainly by activated acrophages . In vitro, it has diverse biological effects (reviewed by Manogue et Cerami, 1988) including killing of transformed cells (Carswell et al., 1975), stimulation of granulocytes and fibroblasts (Old, 1985; Vilchek et al . , 1986; Beutler and Cerami, 1987) , damage to endothelial cells (Sato et al, 1986) , and anti-parasitic effects (Taverne et al. , 1984) . In vivo, it plays a key role as an endogenous mediator of inflammatory, immune and host defence functions and it is involved in a number of pathological conditions in man and mouse such as septic shock, cachexia, capillary leak syndrome, hemorhagic necrosis of multiple organs, etc. It is capable of acting independently and in conjunction with other factors affecting a whole plethora of different body functions . These effects can either be beneficial or life-threatening to the host. Some of these effects are direct, others may be mediated via the induction of other secreted factors. The biological effects of TNF-α are mediated via binding to specific cell surface receptors . The primary structure of human and mouse TNF-α (Pennica et al., 1984; Fransen et al . , 1985) and of two different TNF- receptors (p55-TNF-R and p75-TNF-R) has been deduced from the nucleotide sequence of the cloned cDNA (De bic et al . , 1990; Loetscher et al . , 1990) . Both receptors bind not only TNF-α but also TNF-β or lymphotoxin with high affinity (Schoenfeld et al . , 1991) . TNF-β is a related lymphocyte product (TNF-α and TNF-β share 32% homology on the amino acid sequence level) that exhibits pleiotropic activities very similar to those of TNF-α. X-ray crystallographic analysis revealed that the tertiary st ucture of both molecules is virtually identical except that the TNF-β tri er creates a molecule that is less elongated than the TNF-α trimer and the latter has a top-region that flares open (Eck et al, 1992) . Besides the cell receptor interaction, TNF-α has been shown to have a lectin-like property for the oligosaccharide ligands chitobiose and an(αl,3) - ( an(αl,6) -Man (Hession et al. , 1987; Sherblom et al. , 1988) . They further demonstrated that the TNF-α protein has at least two different binding sites, one lectin-like and the other directed at cell surface receptors. Several TNF-α mutants were described for which the binding to the cellular TNF- receptor p55 and/or p75 was hampered. All these mutations are located in the lower half of the pyramidal structure of the biologically active TNF-α trimer (Van Ostade et al., 1991; Van Ostade et al. , 1992; EP-A-0 486 908). The availability of recombinant TNF has enhanced its use in tumor therapy. However, the in vivo tumoricidal effects of TNF-α have always been accompanied by toxic side effects. Different approaches were followed to overcome these noxious effects, including the use of monoclonal antibodies as well as fragments thereof, which neutralize the in vi tro and in vivo toxic properties of TNF-α (e.g. EP-A-0 350 690) . In addition to its immunomodulating activity, TNF-α has also been shown to be involved in the control of growth and differentiation of various parasites. Upon infection of the host, parasites are capable of inducing the secretion of different cytokines such as TNF which may affect the course of the disease. For instance, in the case of malaria, TNF-α can be protective in certain circumstances, such as inhibiting parasite survival in rodent malaria (Clark et al. , 1987; Taverne et al., 1987). By contrast, its overproduction can be detrimental to the host and can contribute to the pathology of the disease (Clark, 1987; Grau et al. , 1989) . In schistosomiasis, the parasite uses the host-derived immunoregulatory protein TNF-α as a signal for replication and transmission (Amiri et al., 1992). Moreover, the authors found that the parasite worms required TNF-α for egg laying and for excretion of eggs from the host. In cutaneous leishmaniasis, TNF- α plays a protective role (Titus et al. , 1989; iew et al. , 1990) . African trypanosome species are the etiological agents for African trypanosomiasis, or sleeping sickness, and are a major cause of both human disease ( Trypanosoma brucei rhodesiense and T. brucei crambiense) and cattle disease (T. brucei brucei , T. evansi , T. vivax, T. con rolense) in Africa. Injected into their mammalian hosts by tsetse flies, trypanosomes remain extracellular bloodstream trypomastigotes throughout their mammalian phase. In trypanosomiasis, it has been shown that rabbits infected with Trypanosoma brucei brucei developed cachexia and suppression of lipoprotein lipase levels (Rouzer and Cerami, 1980) . These parameters are indicators of TNF-α bioactivity (Beutler et al. , 1985), thus suggesting that the parasite infection induced TNF in vivo . Kongshavn et al. (1988) reported that TNF-α inhibited the growth of in vitro cultures of Tr panosoma musculi . but administration of TNF-α in vivo during the course of infection led to a net increase in parasite development. The in vi tro data indicate that a direct interaction between TNF-α and T. musculi can take place. All these experiments point to a direct interaction between host-derived TNF-α and parasites influencing the course and the outcome of parasitemia. Considerable evidence implicates TNF-α is the principle mediator of endotoxic or septic shock (Cerami and Beutler, 1988) . The intravenous injection of LPS (or endotoxin) in animals produces several parameters of the septic shock syndrome, namely, hypotension, decreased systemic vascular resistance, leukopenia, thrombocytopenia, and tissue dammage. In experimental animals, TNF-α produces hypotension, leukopenia, and local tissue necrosis (Okusawa et al. , 1988). Administration of anti-TNF-α antibodies to baboons (Tracey et al. , 1987) or rabbits prevents the shock induced by endotoxin (Mathison et al., 1988) . The term "TNF-induced septic shock" refers to the shock that develops in the presence of severe infection, especially following bacteremia with Gram-negative bacteria and release of endotoxin. It may also be caused by any class of microorganism, including Gram-positive bacteria, viruses, fungi, protozoa, spirochetes and rickettsiae. The pathophysiology of septic shock is very complex (see Harrison's Principles of Internal Medicine, 12th ed, McGraw-Hill, New York, pp. 502-507) . Death is caused by progression to multiple organ failure and circulatory collapse. Sherblom et al. , (1988) have shown that the TNF-α has a lectin-like affinity for the glycoprotein uromodulin. Interestingly, this glycoprotein is able to potently inhibit the TNF-induced shock in vivo, whereas it does not block the tumoricidal activity of TNF-α on L929 cells in vitro . The authors suggested therefore that the lectin-like region of TNF-α is implicated in the toxic effects of TNF-α in septic shock, but not in its tumoricidal activity. The aim of the present invention is to provide TNF-α muteins which have altered lectin-like properties in comparison with those of TNF-α, and/or TNF-α muteins which have a reduced toxic activity in comparison to TNF-α, and/or TNF-α muteins which have altered inflammatory cytokines inducing capacities with respect to TNF-α, and/or TNF-α muteins with reduced metastasis promoting activities with respect to TNF-α, and/or TNF-α muteins which have an extended half-life time with respect to TNF-α. The aim of the present invention is to provide TNF-α muteins which have reduced lectin-like properties in comparison with those of TNF-α, and/or result in reduced cachexia or septic shock upon treatment with toxic concentrations of TNF-α in cytokine therapy, without having lost,their tumoricidal activity. Another aim of the present invention is to provide pharmaceutical compositions comprising said TNF-α muteins. The aim of the present invention is also to provide antisense peptides or antibodies which recognize the TNF-α lectin-like epitope for use in the preparation of a medicament for treating endogenous toxic concentrations of TNF-α due to sepsis, septic shock, Gram negative sepsis, endotoxic shock, toxic shock syndrome, cachexia, microbial infections, rheumatoid arthritis, inflammatory conditions, respiratory distress syndrome, pulmonary fibrosis, infections, graft-versus-host- disease, reperfusion damage such as myocardial ischaemia, AIDS, cancer, cerebral malaria, immunosuppression, etc. Another aim of the present invention is to use said antibodies or said antisense peptides, in addition to complete TNF-α, for the preparation of a medicament for treating tumors, with said medicament having reduced toxic side effects and/or altered (i.e. reduced or increased) lectin-like effects and/or altered inflammatory cytokines inducing capacities, and/or altered adhesion molecules inducing capacities, and/or an increased half-life with respect to TNF-α in the absence of said antibodies or antisense peptides. The present invention relates more particularly to a tumor necrosis factor mutein characterized in that the TNF-α amino acid sequence is mutated, or deleted totally or partially, in the region extending from amino acid position at 101 to 116 in such a way that.* - either the lectin-like activities are modulated with respect to TNF-α, - and/or the toxic activity is reduced with respect to TNF-α, - and/or the inflammatory cytokines inducing capacities are modulated with respect to TNF-α, - and/or the adhesion molecules inducing capacities are modulated with respect to TNF-α, - and/or the metastasis promoting activity is reduced with respect to TNF-α, - and/or the half life time is increased with respect to TNF-α, and providing that these TNF-α muteins have preferentially retained the tumoricidal activity of TNF-α, and providing that said TNF muteins are different from human TNF-α wherein amino acids 1 to 8 are replaced by a sequence within the region spanning amino acids 5 to 30 of laminin, and providing that said TNF muteins are different from human TNF-α wherein amino acid position 101 is Ser, hTNF-α wherein amino acid position 102 is Arg or deleted, hTNF-α wherein amino acid position 103 is Trp, hTNF-α wherein amino acid position 105 is Pro, hTNF-α wherein amino acid position 105 is lie, hTNF-α wherein amino acid position 105 is lie and position 44 is Cys, hTNF-α wherein amino acid position 106 is Ser, hTNF-α wherein amino acid position 106 is Ser and position 131 is Cys, hTNF-α wherein amino acid position 108 is Phe, hTNF-α wherein amino acid position 110 is Lys, hTNF-α wherein amino acid positions 111 to 112 are deleted, hTNF-α wherein amino acid position 112 is deleted or Met, hTNF-α wherein amino acid position 111 is deleted and amino acid positions 109 and 120 are respectively Gin and His, hTNF-α wherein amino acid position 115 is lie or Cys, hTNF-α wherein amino acid position 116 is Lys, His or Val, hTNF-α wherein amino acid positions 115-116 are Ile-Lys,* and with said TNF muteins possibly containing in their peptidic chain outside the region spanning amino acids 101 to 116 of TNF- α, additional modifications consisting of substitutions and/or deletions and/or additions of one or several amino acid residues, and with said muteins being characterized in that they have retained the aforementioned activities; or a pharmaceutically acceptable salt thereof. It is to be understood that the TNF-α muteins according to the present invention are characterized in that they have at least one of the following effects: - lectin-like activities which are modulated (i.e. reduced or increased) , preferably reduced, from 5 to 100%, preferably at least 25%, more preferably at least 50%, most preferably at least 75%, as compared with lectin-like activities observed for TNF-α; or, - a toxic effect which is reduced from 5 to 50%, preferably at least 10%, more preferably at least 25%, most preferably at least 35% as compared with the toxic effect of TNF-α,* or, - inflammatory cytokines inducing capacities which are modulated (i.e. either reduced or increased), preferably reduced, from 5 to 100%, preferably at least 25%, more preferably at least 50%, most preferably at least 75% with respect to TNF-α; or, - adhesion molecules inducing capacities which are modulated (i.e. either reduced or increased), preferably reduced, from 5 to 100%, preferably at least 25%, more preferably at least 50%, most preferably at least 75% with respect to TNF-α,* or, - a metastasis promoting activity which is reduced from 5 to 100%, preferably at least 25%, more preferably at least 50%, most preferably at least 75% with respect to TNF-α, • or, - a half life time that is increased from 5 to 100%, preferably at least 25%, more preferably at least 50%, most preferably at least 75% with respect to TNF-α; or, - the tumoricidal activity of TNF-α being preferentially retained by said muteins. The tumoricidal activity is considered to be retained within the meaning of the present invention when said TNF-α muteins have a tumoricidal activity of at least 75%, preferably at least 85%, more preferably at least 95% and even more preferably at least 100% of the tumoricidal activity of TNF-α which has not been mutated in the region spanning amino acids 101 to 116. It is, however, also possible that TNF-muteins according to the present invention have largely lost the tumoricidal activities of TNF-α, whilst in addition having at least one, or preferably a combination, of any of the aforementioned activities of the TNF muteins according to the invention which are modulated (i.e. increased or reduced) . For instance, TNF-muteins which have increased lectin-like activities and/or a reduced toxicity, and/or a reduced metastasis promoting activity, and/or an extended half life, and which can no longer be classified as having a tumoricidal activity are also contemplated within the present invention. It is also to be understood that the TNF-muteins according to the present invention will mostly show a combination of at least two of the aforementioned effects. Illustrations of preferred TNF-muteins according to the present invention are given below and in the Examples section. The term "toxic activity" refers to the in vivo TNF- or LPS- induced septic shock effect of TNF-α as explained above, and may be measured in vivo on the basis of the increased survival time of C57BL/6 mice, upon injection with fixed dosages of the TNF-α mutein, as compared with that in C57BL/6 mice injected with the same dosages of unmodified TNF-α,* or by any other technique known in the art. The term "tumoricidal activity" refers to the in vivo tumor cell killing activity of TNF-α, which may be measured by means of any assay comprised in the art. The term "cytotoxic or cytolytical activity" refers to the in vitro tumor cell killing activity of TNF-α. In the Examples section of this application an in vitro assay is reported to measure the cytotoxic activity of TNF-α on L929 TNF-sensitive .fibrosarcoma cells. An in vivo assay to evaluate the tumoricidal activity of TNF-α on HT-29 tumors in nude mice, and by means of the B16B16 melanoma test system, are also reported in the Examples section. The term "lectin-like activities" of the present invention refers to the lectin-like properties of TNF-α. The lectin-like properties of TNF-α are for instance those described by Hession et al. (1988) and Sherblom et al. (1988) and include any biological effect mediated by any lectin-carbohydrate interaction located in the region extending from amino acids 101 to 116 of TNF-α. The "lectin-like activities" of the TNF-α muteins of the invention may be measured as the percentage of inhibition of trypanocidal activity of the TNF mutein, upon prior incubation of said TNF mutein with the oligosaccharide sugar N,N'- diacetylchitobiose as described in the Examples section; or by means of any other assay comprised in the art. The term "trypanocidal activity" refers to the trypanocidal activity of TNF-α located in the region extending from amino acid positions 101 to 116 of TNF-α may be measured by means of an in vitro or an in vivo trypanocidal activity assay as extensively explained in the Examples section. The term "inflammatory cytokines inducing capacities" of TNFα within the meaning of the present invention refers to the stimulation capacity of TNF-α of cytokines such as ILlα, ILlβ, ■IL8, TNF, IL6 and other molecules known to have a role in inflammatory processes by endothelial cells, said capacities being located in the region extending from amino acids 101 to 116 of TNF-α. The term "adhesion molecules inducing capacities" of TNFα within the meaning of the present invention refers to the stimulation of adhesion molecules capacity of TNFα, such as the stimulation of ICAM-1, ICAM-2, ECAM-1 (E-selectin) , P-selectin, Endoglin, VCAM-1, VLA Integrin (Bl-integrin family), CD36, CD9, CD31, GlyCAM-1, which mediate adhesion events necessary to mount inflammatory reactions, said capacities being located within the region extending from amino acid positions 101 to 116 of TNFα. The term "metastasis promoting activity" within the meaning of the present invention refers to negative in vivo side effects of TNF-α exerted against tumor cells and resulting in a net increase/growth of the number of metastase such as reviewed by Orosz et al. , 1993, located within the region extending from amino acid positions 101 to 116 of TNFα. The term "half-life" refers to the in vivo measured time during which the TNF-α muteins of the invention remain in circulation and are detectable in body fluids. The inhibition / stimulation of these TNF-α muteins on the inflammatory cytokines inducing capacities of TNFα may be measured in vivo by any technique known in the art, more particularly any of the techniques disclosed in the examples section of the present invention. The inhibition / stimulation of these TNF-α muteins on the adhesion molecules inducing capacities of TNF-α may be measured in vivo by any technique known in the art. The inhibition of metastasis promoting activity by these TNF-α muteins may be measured in vivo by any technique known in the art, or as disclosed in the examples section. The increase of the half life time by these TNF-α muteins may be measured in vivo by any technique known in the art, or as disclosed in the examples section. The term "TNF-α" refers to polypeptides of human origin containing 157 amino acid residues, as disclosed by Pennica et al. (1984) , namely: 5 10 15 1 Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His 16 Val Val Ala Asn Pro Gin Ala Glu Gly Gin Leu Gin Trp Leu Asn 31 Arg Arg Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp 46 Asn Gin Leu Val Val Pro Ser Glu Gly Leu Tyr Leu lie Tyr Ser 61 Gin Val Leu Phe Lys Gly Gin Gly Cys Pro Ser Thr His Val Leu 76 Leu Thr His Thr lie Ser Arg lie Ala Val Ser Tyr Gin Thr Lys 91 Val Asn Leu Leu Ser Ala lie Lys Ser Pro Cys Gin Arg Glu Thr 106 Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro lie Tyr Leu 121 Gly Gly Val Phe Gin Leu Glu Lys Gly Asp Arg Leu Ser Ala Glu 136 lie Asn Arg Pro Asp Tyr Leu Asp Phe Ala Glu Ser Gly Gin Val 151 Tyr Phe Gly He He Ala Leu or as disclosed by Marmenout et al. (1985), or by Wang et al. (1985), or Shirai et al. (1985). The term "TNF-α" of the present invention also refers to polypeptides of mouse origin containing 156 amino acid residues, as disclosed by Fransen et al. (1985) namely: 5 10 15 1 Leu Arg Ser Ser Ser Gin Asn Ser Ser Asp Lys Pro Val Ala His 16 Val Val Ala Asn His Gin Val Glu Glu Gin Leu Glu Trp Leu Ser 31 Gin Arg Ala Asn Ala Leu Leu Ala Asn Gly Met Asp Leu Lys Asp 46 Asn Gin Leu Val Val Pro Ala Asp Gly Leu Tyr Leu Val Tyr Ser 61 Gin Val Leu Phe Lys Gly Gin Gly Cys Pro Asp Tyr - Val Leu 76 Leu Thr His Thr Val Ser Arg Phe Ala He Ser Tyr Gin Glu Lys 91 Val Asn Leu Leu Ser Ala Val Lys Ser Pro Cys Pro Lys Asp Thr 106 Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr Glu Pro He Tyr Leu 121 Gly Gly Val Phe Gin Leu Glu Lys Gly Asp Gin Leu Ser Ala Glu 136 Val Asn Leu Pro Lys Tyr Leu Asp Phe Ala Glu Ser Gly Gin Val 151 Tyr Phe Gly Val He Ala Leu or as disclosed by Pennica et al. (1985) , which at least contain the peptide sequence Thr Pro Glu Gly Ala Glu (SEQ ID NO 23) in their peptidic chain. The expression "TNF-α amino acid sequence which is mutated in the region extending from amino acid position 101 to 116" refers to either deletions, insertions and/or substitutions in the region of amino acid positions 101 to 116 of TNF-α (including both positions 101 and 116) . It is to be understood that the result of all of these deletions is such that at least one of the effects mentioned-above, and preferably combinations of at least two such effects of TNF-α, ascribed to the region covering amino acid positions 101 to 116 of TNF-α, are modulated and by preference are strongly reduced or abolished in the case of life- threatening lectin-like effects (toxic effect, inflammatory cytokines inducing activities, adhesion molecules inducing activities) , metastasis promoting effects of TNF-α, or are by preference increased in the case of half-life times and beneficial lectin-like activities (such as induction of cytokines or adhesion molecules which are beneficial to the envisaged treatment) . For TNF-β, the above described septic shock or cachexia effects are not found. The term "TNF-β" refers to tumor necrosis factor β or lymphotoxin (Gray, 1987) . TNF-β and TNF-α are members of a duplicated gene family which show 30 to 32% homology at the amino acid level and share many biological activities, including the tumoricidal activity against L929 cells. Despite the fact that both cytokines share the same cell-surface receptor (Aggarwal et al. , 1985) , important differences exist between TNF- α and TNF-β, both with regard to protein sequence and gene regulation. There are also distinct differences in the mode of secretion in that TNF-α is a membrane protein (Kriegler et al. , 1988) , whereas TNF-β is a secreted protein. Table I gives an overview of the amino acid substitutions which could be the basis of some of the additional modifications in the TNF-α muteins in the region outside the region extending from amino acid positions 101 to 116 as defined above. TABLE I Amino acids Synonymous groups Ser Ser , Thr, Gly, Asn Arg Arg , His, Lys, Glu, Gin Leu Leu He, Met, Phe, Val, Tyr Pro Pror Ala, Thr, Gly Thr Thr Pro, Ser, Ala, Gly, His, Gin Ala Ala Pro, Gly, Thr Val Val Met, He, Tyr, Phe, Leu, Val Gly Gly Ala, Thr, Pro, Ser He He Met, Leu, Phe, Val, He, Tyr Phe Phe Met, Tyr, He, Leu, Trp, Val Tyr Tyr Phe, Trp, Met, He, Val, Leu Cys Cys Ser, Thr His His Gin, Arg, Lys, Glu, Thr Gin Gin Glu, His, Lys, Asn, Thr, Arg Asn Asn Asp, Ser, Gin Lys Lys Arg, Glu, Gin, His Asp Asp, Asn, Glu Glu Glu, Gin, Asp, Lys, Asn, His, Arg Met Met, He, Leu, Phe, Val It is reported in the example section of the present invention that TNF-α-derived synthetic peptides located in the region extending from amino acid positions 101 to 116 of TNF-α as indicated in the sequences above, and which will be further referred to as the "TNF-α tip peptides", and which can be represented by the following general formula: (Yl-Y2-Y3) n -Thr-Xl-Glu-X2-X3-Glu- (Y4-Lys-Pro-Trp-Tyr) n (SEQ ID NO 1) , wherein, - n represents 0 or l, - XI can represent Pro, Ala, Thr, or Gly; - X2 can represent Gly, Ala, Thr, Pro, or Ser; - X3 can represent Ala, Pro, Gly, or Thr,* - Yl can represent Pro or Gin, - Y2 can represent Lys or Arg ( - Y3 can represent Asp or Glu, - and, Y4 can represent Leu or Ala have a direct trypanocidal activity against the pleomorphic bloodstream forms of the African trypanosomes Trγpanosoma brucei brucei and Trypanosoma brucei rhodesiense . It is to be understood that the TNF-α amino acid position numbering in the present application refers to the numbering as given in the sequences above. In this respect, it is to be noted that the region covering amino acids 101 to 116 of human TNF-α corresponds to amino acids 100 to 115 of mouse TNF-α, since in the sequences given above, a space has been introduced in the mouse TNF-α amino acid sequence position 73 in order to align it with the human TNF-α amino acid sequence. Moreover, it is shown in the examples section that the trypanocidal activity of the TNF-α "tip-region" can be inhibited by preincubation with N,N' -diacetylchitobiose, for which TNF-α has a lectin-like activity (Hession et al. , 1987; Sherblom et al.,1988) . Therefore, the tip region of TNF-α is shown to be implicated in the lectin-like activities of the cytokine. Since the glycoprotein uromodulin, which binds TNF-α via its lectin-like domain, can inhibit lipopolysacharide (LPS) -induced septic shock, caused by TNF-α, but can not inhibit the TNF-α tumoricidal effect on L929 cells, the lectin-like region of TNF-α, and consequently the tip-region of TNF-α, are shown by the present inventors to be implicated in the toxic side effects of the cytokine in vivo. Consequently, as illustrated in the examples section of the present invention, TNF-α muteins (extending from positions 101 to 116 'of TNF-α) were constructed in the tip-region which are characterized in that either the lectin-like activities are reduced with respect to TNF-α, or the toxic activity is reduced with respect to TNF-α; or the half life iε increased with respect to TNF-α,* or the metastasis promoting activities are reduced with respect to TNF-α, or the inflammatory cytokines inducing capacities are reduced with respect to TNF-α, or a combination of these effects are reduced/increased with respect to TNF-α and with said muteins being further characterized in that they have not lost the tumoricidal activity of TNF-α,* as detailed above. Consequently the present invention relates more particularly to a TNF mutein as defined above, further characterized in that the lectin-like activitieε are modulated with respect to TNF-α. According to a preferred embodiment, the present invention relates to a TNF mutein as defined above, further characterized in that the lectin-like activities are reduced with respect to TNF-α. It is extensively illustrated in the examples section of the present invention that the region spanning amino acidε 101 to 116, and more particularly 105 to 110, iε involved not only in the trypanocidal but alεo in the lectin-like activities of TNF-α. The lectin-like activities were measured indirectly via the inhibition of the trypanocidal activity of TNF-α. According to a posεible embodiment, the preεent invention alεo relateε to a TNF mutein aε defined above, further characterized in that the lectin-like activitieε are increased with respect to TNF-α. TNF muteins having increased lectin-like activities may be beneficial to certain envisaged treatments. According to another preferred embodiment, the present invention relates to a TNF mutein as defined above, further characterized in that the toxic activity is reduced with respect to TNF-α. It is illustrated in the exampleε εection of the present invention that mutations to an Ala at positions T(105), E(107) and/or E(110) and a deletion of the region extending from amino acids 105 to 110 of mTNFα leadε to mTNF-α muteinε with a lower LD50 value aε meaεured in vivo. According to yet another embodiment, the preεent invention relates to a TNF mutein aε defined above, further characterized in that the inflammatory cytokine inducing capacitieε are modulated with reεpect to TNF-α. According to a preferred embodiment, the preεent invention relates to a TNF mutein as defined above, further characterized in that the inflammatory cytokine inducing capacities are increased with reεpect to TNF-α. Aε εhown in the exampleε section of the preεent invention, mTNF-α muteins bearing a deletion of the region spanning amino acidε 105-110 of mTNF-α εhow a higher induction of human interleukin-6 on human microvascular endothelial cells of the brain in vivo than wild-type mTNFα. According to another possible embodiment, the present invention relateε to a TNF mutein as defined above, further characterized in that the inflammatory cytokine inducing capacities are reduced with respect to TNF-α. It is shown in the examples section that T(105)A and P(106)A mTNF-α muteins as well a deletion mutant of mTNFα in the region extending from amino acids 105 to 110 induce consistently less human interleukin 8 (IL8) than wild-type TNF-α on human endothelial cells as measured in vivo. According to yet another embodiment, the present invention relates to a TNF mutein aε defined above, further characterized in that the adhesion molecule inducing capacities are modulated with respect to TNF-α. According to another preferred embodiment, the present invention relates to a TNF mutein as defined above, further characterized in that the adhesion molecule inducing capacitieε are reduced with respect to TNF-α. According to another posεible embodiment, the present invention refers to a TNF mutein as defined above, further characterized in that the adhesion molecule inducing capacities are increased with respect to TNF-α. According to yet another preferred embodiment, the present invention relates to a TNF mutein as defined above, further characterized in that the metastasis promoting activity is reduced with respect to TNF-α. Aε illustrated in the examples section, a mTNFα mutein carrying a deletion of the region extending from amino acids 105 to 110 εhowε a considerably lower in vivo metastaεiε enhancing potential compared to wild-type mTNFα in a murine lung carcinoma cell line model. According to a preferred embodiment, the present invention relates to a TNF mutein having at least one of the afore¬ mentioned effects, further characterized in that the tumoricidal activity is retained with respect to TNF-α. As εhown in the exε pleε εection, all up till now conεtructed mTNFα muteinε have retained their tumoricidal activity in comparison to wild-type mouse TNFα. According to yet another poεεible embodiment of the preεent invention, any of the TNF muteins having at least one of the effectε aε defined above are further characterized in that their tumoricidal activity is reduced with respect to TNF-α. In view of posεible treatmentε with the TNF-α muteinε on the basis of their altered lectin-like, or inflammatory cytokines inducing, or adhesion molecules inducing propertieε, the loss of the tumoricidal effect by certain TNF muteins of the invention may be desirable. According to yet another preferred embodiment, the preεent invention relates to a TNF mutein as defined above, further characterized in it shows an increased half life time with reεpect to TNF-α. As shown in the examples εection, the tip deleted (amino acidε 105-110) mTNFα muteins showε an increased half life as measured by means of the L929 cytotoxic assay and the indirect ELISA compared to wild-type mTNFα. According to a preferred embodiment, the present invention relateε to any TNF mutein as defined above, characterized in that at least part of the region extending from amino acid positionε 101 to 116 of TNF-α, or the complete region correεponding to amino acid poεitionε 101 to 116 of TNF-α has been deleted, and preferably at least the region covering amino acid positions 105 to 110 has been deleted. According to the latter preferred embodiment said TNF muteins should be different from human TNF-α wherein amino acids 1 to 8 are replaced by a sequence within the region spanning amino acids 5 to 30 of laminin, and providing that said TNF muteins are different from human TNF-α wherein amino acid position 105 is Pro, hTNF-α wherein amino acid position 105 is He, hTNF-α wherein amino acid position 105 is He and position 44 is Cys, hTNF-α wherein amino acid position 106 is Ser, hTNF-α wherein amino acid position 106 is Ser and position 131 is Cys, hTNF-α wherein amino acid position 108 iε Phe, hTNF-α wherein amino acid poεition 110 iε Lyε, and hTNF-α wherein amino acid poεitionε 109 and 120 are reεpectively Gin and His and amino acid 111 is deleted, and may contain subεtitutionε aε set out in Table 1, in the region of TNF-α outside the region spanning from amino acid position 105 to amino acid position 110. According to a preferred embodiment, the present invention relates to a TNF mutein according to as defined above, characterized in that at least one of the amino acids in the region extending from amino acids 101 to 116 of TNF-α has been mutated or deleted more particulary at least one of the amino acids in the region extending from amino acids 105 to 110. According to a particular embodiment, the present invention relates to TNF muteins wherein the region between amino acid positions 101 and 116 has been mutated in such a way that the amino acids Thr(105), Glu(107) and/or Glu(llO) have been mutated to an Alanine reεidue, aε demonstrated in the examples section. Although the examples given relate to mouεe TNF-α muteinε, alεo human TNF-α muteinε are contemplated within the present invention. According to yet another embodiment, the preεent invention relateε to a nucleic acid εequence encoding any of the TNF mutein polypeptides as defined above. According to yet another embodiment, the present invention relates to a process for the preparation of the TNF mutein polypeptides as defined above, compriεing the εtepε of: - tranεformation of an appropriate cellular hoεt with a vector, particularly a plaεmid, a coεmid, a phage or a virus, in which a nucleic acid εequence aε defined above coding for at least one of the TNF mutein polypeptides as defined above has been inserted (insert) under the control of the appropriate regulatory elements, particularly a promoter recognized by the polymeraεes of the cellular host and, in the case of a procaryotic host, an appropriate ribosome binding εite (RBS) , enabling the expreεεion in εaid cellular hoεt of εaid nucleic acid sequence, - culture of said tranεformed cellular hoεt under conditions enabling the expreεεion of εaid inεert. The polypeptideε according to the preεent invention can be prepared via recombinant DNA technology. In order to carry out the expreεεion of the polypeptideε of the invention in bacteria εuch as E. coli or in an eukaryotic cell such aε Saccharomyces cerevisiae, or in cultured vertebrate or invertebrate hoεts such as insect cells, Chinese Hamεter Ovary (CHO) , COSl, BHK, and MDCK. The polypeptideε prepared according to thiε process of the invention will normally contain only naturally occuring L-amino acids. If required, D-amino acidε can be incorporated into the polypeptideε of the invention according to any technique known in the art, provided their incorporation into εaid peptideε doeε not interfere with the lectin-like, or toxic, or tumoricidal propertieε of the TNF muteins according to the invention. The present invention relates also to TNF muteins characterized in that the TNF-α amino acid sequence iε mutated, or deleted totally or partially, in the region extending from amino acid poεition at 101 to 116 in εuch a way that: - either the lectin-like activitieε are modulated with reεpect to TNF-α, - and/or the toxic activity iε reduced with reεpect to TNF-α, - and/or the inflammatory cytokineε inducing capacitieε are modulated with reεpect to TNf-α, - and/or the adheεion moleculeε inducing capacitieε are modulated with reεpect to TNF-α, - and/or the metastasiε promoting activity iε reduced with reεpect to TNF-α, - and/or εhow an increaεed half life time with respect to TNF-α, and providing that theεe TNF-α muteins have preferentially retained the tumoricidal activity of TNF-α, and with εaid TNF muteinε possibly containing in their peptidic chain outside amino acid region 101 to 116 of TNF-α, additional modifications conεiεting of εubεtitutionε and/or deletions and/or additionε of one or εeveral amino acid reεidueε, and with εaid muteinε being characterized in that they have retained the aforementioned activitieε; or a pharmaceutically acceptable εalt thereof, for treating illnesseε and pathological conditionε, εuch as sepsis, septic shock, Gram negative sepsis, endotoxic shock, toxic shock εyndrome, cachexia, microbial infections, rheumatoid arthritiε, inflammatory conditionε, reεpiratory diεtreεε syndrome, pulmonary fibrosis, infections, graft-versus-host- disease, reperfusion damage such as myocardial ischaemia, AIDS, cancer, immunosuppression, cerebral malaria, etc. It is to be underεtood that the TNF-α muteinε according to the present invention may advantageouεly be used for the fact that they have a tumoricidal activity, while they lack either the life-threatening toxic, or the lectin-like properties of TNF-α, or the metastasis promoting effects of TNF-α, or the inflammation cytokines inducing capacities of TNF-α or mixture of theεe effectε, and/or because they have an increased half life compared to TNF-α, and/or because they have increaεed beneficial lectin- like activitieε and/or inflammatory cytokines inducing activities, and/or asheεion moleculeε inducing activities compared to TNF-α. According to an advantageouε embodiment, εaid muteinε for treating the above-εpecified illneεεes are preferentially different from from human TNF-α wherein amino acids 1 to 8 are replaced by a sequence within the region εpanning amino acids 5 to 30 of laminin, and providing that said TNF muteins are preferentially different from human TNF-α wherein: amino acid position 101 is Ser, hTNF-α wherein amino acid position 102 iε Arg or deleted, hTNF-α wherein amino acid poεition 103 iε Trp, hTNF-α wherein amino acid poεition 105 iε Pro, hTNF-α wherein amino acid poεition 105 is He, hTNF-α wherein hTNF-α amino acid position 105 iε He and poεition 44 iε Cyε, hTNF-α wherein amino acid poεition 106 is Ser, hTNF-α wherein amino acid position 106 is Ser and position 131 iε Cyε, hTNF-α wherein amino acid poεition 108 is Phe, hTNF-α wherein amino acid position 110 iε Lys, hTNF-α wherein amino acid positions 111 to 112 are deleted, hTNF-α wherein amino acid position 112 is deleted or Met, hTNF-α wherein amino acid position 111 iε deleted and amino acid poεitionε 109 and 120 are respectively Gin and His, hTNF-α wherein amino acid poεition 115 is He or Cys, hTNF-α wherein amino acid position 116 is Lys, His or Val, hTNF-α wherein amino acid positions 115-116 are Ile-Lys. According to yet another advantageouε embodiment of the preεent invention, the TNF muteinε uεed for treating the above- εpecified illneεses contain either a mutation of one or more amino acids or a deletion of at least part of the region spanning amino acids 105 to 110 of TNF-α. Particularly preferred examples as well as their biological effects are diεcuεsed in the examples εection. According to a preferred embodiment, the present invention relates to a pharmaceutical composition, containing, as active subεtance, at leaεt anyone of the TNF muteinε aε defined above, in aεεociation with a pharmaceutical acceptable vehicle. The mode of adminiεtration of these pharmaceutical compositions is enteral or preferably parenteral, i.e., intravenouε, intraperitoneal, intramuεcular, or subcutaneous, with the intravenous route being preferred. The active substances of these pharmaceutical compositionε may be administered alone, without a carrier vehicle,* however, they may also be administered with pharmaceutically acceptable non-toxic carriers or diluents, the proportions of which are determined by the suitability and chemical nature of the particular carrier. According to an advantageouε embodiment, the above-mentioned pharmaceutical compoεitionε contain muteinε which are preferably different from human TNF-α wherein: amino acids 1 to 8 are replaced by a εequence within the region spanning amino acidε 5 to 30 of laminin, and providing that said TNF muteinε are different from human TNF-α wherein amino acid poεition 101 is Ser, hTNF-α wherein amino acid position 102 is Arg or deleted, hTNF-α wherein amino acid position 103 iε Trp, hTNF-α wherein amino acid poεition 105 is Pro, hTNF-α wherein amino acid position 105 is He, hTNF-α wherein amino acid poεition 105 is He and position 44 iε Cyε, hTNF-α wherein amino acid poεition 106 iε Ser, hTNF-α wherein amino acid poεition 106 iε Ser and poεition 131 is Cyε, hTNF-α wherein amino acid position 108 is Phe, hTNF-α wherein amino acid position 110 is Lys, hTNF-α wherein amino acid poεitionε 111 to 112 are deleted, hTNF-α wherein amino acid poεition 112 iε deleted or Met, hTNF-α wherein amino acid position 111 is deleted and amino acid positions 109 and 120 are respectively Gin and His, hTNF-α wherein amino acid position 115 is He or Cys, hTNF-α wherein amino acid position 116 is Lyε, Hiε or Val, hTNF-α wherein amino acid poεitionε 115-116 are Ile-Lyε. According to an advantageous embodiment, the above-mentioned pharmaceutical compositionε contain muteins which can be human TNF-α wherein: amino acids 1 to 8 are replaced by a εequence within the region εpanning amino acidε 5 to 30 of laminin, and providing that εaid TNF muteinε are different from human TNF-α wherein amino acid poεition 101 iε Ser, hTNF-α wherein amino acid poεition 102 is Arg or deleted, hTNF-α wherein amino acid position 103 is Trp, hTNF-α wherein amino acid position 105 is Pro, hTNF-α wherein amino acid poεition 105 iε He, hTNF-α wherein amino acid poεition 105 iε He and poεition 44 is Cys, hTNF-α wherein amino acid position 106 iε Ser, hTNF-α wherein amino acid ' poεition 106 iε Ser and poεition 131 iε Cys, hTNF-α wherein amino acid position 108 iε Phe, hTNF-α wherein amino acid position 110 is Lys, hTNF-α wherein amino acid positions 111 to 112 are deleted, hTNF-α wherein amino acid position 112 iε deleted or Met, hTNF-α wherein amino acid poεition 111 iε deleted and amino acid positions 109 and 120 are respectively Gin and Hiε, hTNF-α wherein amino acid poεition 115 iε He or Cyε, hTNF-α wherein amino acid position 116 is Lys, His or Val, hTNF-α wherein amino acid positions 115-116 are Ile-Lys. According to yet another advantageous embodiment of the present invention, the TNF muteins uεed for treating the above- εpecified illnesses contain either a mutation of one or more amino acids or a deletion of at leaεt part of the region εpanning amino acids 105 to 110 of TNF-α. Particularly preferred examples as well as their biological effects are diεcuεsed in the examples εection. The preεent invention relateε alεo to the use of a TNF mutein characterized in that the TNF-α amino acid sequence is mutated, or deleted totally or partially, in the region extending from amino acid position at 101 to 116, more particularly in the region extending from amino acid positions 105 to 110, in such a way that: - either the lectin-like activities are modulated with reεpect to TNF-α, - and/or the toxic activity iε reduced with reεpect to TNF-α, - and/or the inflammatory cytokineε inducing capacities are modulated with respect to TNf-α, - and/or the adheεion moleculeε inducing capacitieε are modulated with reεpect to TNF-α, - and/or the metastasiε promoting activity iε reduced with respect to TNF-α, - and/or show an increased half life time with respect to TNF-α, and providing that these TNF-α muteinε have preferentially retained the tumoricidal activity of TNF-α, and with εaid TNF muteinε possibly containing in their peptidic chain outside amino acid region 101 to 116 (or reεpectively 105 to 110) of TNF-α, additional modifications consiεting of εubεtitutions and/or deletions and/or additions of one or several amino acid residues, and with said muteinε being characterized in that they have retained the aforementioned activitieε; or a pharmaceutically acceptable εalt thereof, for the preparation of a medicament for treating illneεεeε and pathological conditionε, εuch as, sepsis, septic shock, Gram negative εepεiε, endotoxic εhock, toxic εhock εyndrome, cachexia, microbial infections, rheumatoid arthritis, inflammatory conditions, respiratory distresε εyndro e, pulmonary fibroεiε, infectionε, graft-versus- hoεt-diεeaεe, reperfuεion damage εuch as myocardial ischaemia, AIDS, cancer, immunoεuppression, cerebral malaria, etc. It is to be underεtood that the TNF-α muteinε according to the preεent invention may especially be used for the fact that they may have a tumoricidal activity, while they lack either the toxic, and/or the lectin-like properties of TNF-α, and/or the metaεtaεiε promoting effects of TNF-α, and/or the inflammatory cytokines inducing capacitieε of TNFα, and/or the adheεion moleculeε inducing capacities of TNFα, or mixture of these effects, and/or because they have an increased half life compared to TNF-α. According to another embodiment, the present invention relates to the use of an antibody specifically detecting an epitope reεiding in the region encompassing amino acids 101 to 116 of TNF-α, preferably in the region encompassing amino acids 105 to 110 of TNFα more particularly a monoclonal antibody, characterized in that it: - either modulates the lectin-like activities of TNF-α, - and/or inhibits the toxic activity of TNF-α, - and/or modulates the inflammatory cytokines inducing capacitieε of TNf-α, - and/or modulates the adhesion molecules inducing capacities of TNF-α, - and/or inhibits the metaεtaεis promoting activity of TNF-α, for the preparation of a medicament used for treating TNF-α- induced septic shock, or for treating any pathological condition aεεociated with the in vivo lectin-like activitieε of TNF-α, εuch aε for treating εepεiε, εeptic εhock, Gram negative sepsis, endotoxic shock, toxic shock εyndrome, cachexia, microbial infections, rheumatoid arthritis, inflammatory conditions, respiratory distreεε εyndrome, pulmonary fibroεiε, infectionε, graft-verεuε-hoεt-diεeaεe, reperfuεion damage εuch aε myocardial iεchaemia, AIDS, cancer, immunosuppreεεion, cerebral malaria, etc. It iε to be underεtood that the antibodieε according to the present invention are characterized in that they have at least one of the following effects: - they modulate (i.e. inhibit or stimulate), preferably inhibit, the lectin-like effects of TNF-α preferentially from 5 to 100%, preferably at least 25%, more preferably at least with 50%, most preferably with at least 75%; or, - they inhibit the toxic effect of TNF-α with from 5 to 50%, preferably at leaεt 10%, more preferably with at leaεt 25%, most preferably with at least 35%; or, - they modulate (i.e. inhibit or stimulate), preferably inhibit, the inflammatory cytokines inducing capacities of TNF-α from 5 to 100%, preferably with at least 25%, more preferably with at least 50%, most preferably with at leaεt 75%; or, - they modulate (i.e. inhibit or εtimulate) , preferably inhibit, the adheεion moleculeε inducing capacitieε of TNF-α from 5 to 100%, preferably with at least 25%, more preferably with at least 50%, most preferably with at leaεt 75%; or, - they inhibit the metaεtaεis promoting activity of TNF-α from 5 to 100%, preferably with at least 25%, more preferably with at least 50%, most preferably with at least 75% with respect to TNF- α. The inhibition / stimulation of the lectin-like activities of TNF-α by the antibodies of the invention iε measured as the percentage of inhibition of trypanocidal activity upon prior incubation of TNF-α with said antibody of the invention in the preεence and/or abεence of N,N' -diacetylchitobioεe; or by any other method known in the art. The inhibition of the toxic activity of TNF-α by the antibodieε of the invention may be meaεured by the increaεe in survival time of Balb/C mice, upon treatment with LPS in the presence of different concentrationε of the antibody of the invention, aε illuεtrated in the Exampleε section. The inhibition of the tumoricidal activity may be meaεured by meanε of any aεsay compriεed in the art which measures this activity in vivo on TNF-εenεitive tumor cellε. In the example section of this application, an in vitro assay to measure the cytotoxic (cytolytic) activity of TNF-α on L929 TNF-εenεitive fibroεarcoma cellε iε reported. An in vivo aεεay to evaluate the tumoricidal activity of TNF-α on HT-29 tumorε in nude mice, and by meanε of the B16B16 melanoma test system, are also reported in the examples section. The inhibition / stimulation of theεe antibodies on the inflammatory cytokineε inducing capacities of TNFα may be measured in vivo by any technique known in the art, more particularly any of the techniques disclosed in the examples section of the preεent invention. The inhibition / εtimulation of theεe antibodieε on the adheεion moleculeε inducing capacitieε of TNF-α may be meaεured in vivo by any technique known in the art. The inhibition of metastasis promoting activity by these antibodies of TNF-α may be measured in vivo by any technique known in the art, or as disclosed in the examples section. In order to aεεume inhibition of one or combinationε of any of the mentioned effectε in vivo, the monoclonal antibody of the invention εhould form an immunological complex with the εubregion of the TNF-α "tip-region" responsible for either of the effects of TNF-α. According to a preferred embodiment, the preεent invention relates to the use of an immunological complex, compriεing a monoclonal antibody aε defined above, and TNF-α, for the preparation of a medicament. According to thiε embodiment of the invention, a pharmaceutical compoεition, containing, aε active εubεtance, an immunological complex compriεing a monoclonal antibody aε defined above, and complete TNF-α, in aεεociation with a pharmaceutical acceptable vehicle, iε provided. Inεtead of, or in addition to TNF-α, the above mentioned compoεitions could also contain IL-lα, IL-lβ, IL-6, IFN-gamma, IFN α/β, IL-8, or any other immunoreactive agent. The above-mentioned composition can be used for treating illnesses wherein a treatment with TNF-α is beneficial εuch as tumors. Moreover, the above-mentioned composition may especially be used for the fact that it exterts a tumoricidal activity, while lacking either the toxic, and/or the lectin-like propertieε of TNF-α, and/or the metaεtaεiε promoting effectε of TNF-α, and/or inflammatory cytokines inducing capacities, and/or adheεion moleculeε inducing capacitieε, or mixture of theεe effects, and/or because they have an increased half life compared to TNF- α. Therefore, they are ideally εuited for treating tumorε or cancerε. The antibodies according to this embodiment of the invention may include polyclonal or monoclonal antibodies. The monoclonal antibodies of the invention can be produced by any hybridoma liable to be formed according to clasεical methodε from splenic cells of an animal, particularly of a mouse or rat, immunized against the polypeptides according to the invention, or muteinε thereof, or fragments thereof defined above on the one hand, and of cells of a myeloma cell line on the other hand, and to be selected by the ability of the hybridoma to produce the monoclonal antibodies recognizing the polypeptides which haε been initially uεed for the immunization of the animalε. The antibodieε involved in the invention can be labelled by an appropriate label of the enzymatic, fluoreεcent, or radioactive type. In the caεe of treatment of human illneεses, the monoclonal antibodieε according to thiε preferred embodiment of the invention may be humanized versions of mouse monoclonal antibodieε made by meanε of recombinant DNA technology, departing from parts of mouse and/or human genomic DNA εequenceε coding for H and L chainε or from cDNA clones coding for H and L chains. Alternatively the monoclonal antibodies according to thiε preferred embodiment of the invention may be human monoclonal antibodies. Such human monoclonal antibodies are prepared by EBV immortalization or, for instance, by means of human peripheral blood lymphocyte (PBL) repopulation of severe combined immune deficiency (SCID) mice (for recent review see Duchosal et al. , 1992) . The invention alεo relateε to the uεe of the peptideε or polypeptides of the invention for the selection of recombinant antibodieε by the process of repertoire cloning (Perrson et al. , 1991) . Monoclonal antibodieε can be characterized according to their εubclaεε by known methodε εuch aε Ouchterlony immunodiffuεion. It iε furthermore known in the art that monoclonal antibodies, or fragments thereof, can be modified for various uses, and fragments can be generated which retain the antigen binding properties. Such fragments are commonly generated by, for instance enzymatic digestion of the antibodieε with papain, pepεin, or other proteaεeε. The peptideε according to thiε embodiment of the invention may be circularized before immunization. The expression "circularized" refers to a joining of the N- and C- terminal cyεteine reεidueε by meanε of oxidation at low molar concentration to promote intramolecular diεulfide bridgeε or by any other technique compriεed in the art. If deεired, all above-mentioned peptideε or polypeptides can be provided for with an extra N- and C-terminal cysteine residue, in order to circularize the peptide. The peptides used for immunization are preferably in the form in which they are joined to a biotin molecule, or any other carrier molecule in order to achieve a good immunogenic response. As demonstrated in the Exampleε εection, the biotin molecule may be coupled to the peptide of choice via a linker, εuch aε Gly- Gly, or any other linker compriεed in the art. Furthermore, the peptideε uεed for immunization preferentially contain only naturally occuring L-amino acids. If required, D-amino acids may be incorporated as long as the immunogenic, lectin-like, toxic and/or trypanocidal properties of the peptides have been retained. The TNF-induced septic shock effect may be assayed by means of the increaεe of the εurvival time after injectionε of the antibody to LPS-treated Balb/C mice aε illuεtrated in the Exampleε section. As mentioned above, the tip region of TNF-α may be implicated in the toxic side effects of the cytokine on cells, thereby limiting the wider therapeutic use of TNF-α on normal cells (reviewed by Taniguchi and Sohmura, 1991) . Therefore, antibodies recognizing the tip region of TNF-α can be of particular interest to discriminate between the tumoricidal effect of TNF-α on the one hand, and the trypanolytic and toxic effect of TNF-α on the other. Antibodies recognizing the above- mentioned TNF-α tip region polypeptides of the invention, will be further referred to as anti-tip antibodieε. The preεent invention relateε also to the use of an antisenεe peptide of a peptide comprising at least part of the region extending from amino acids 101 to 116 of TNF-α, preferably compriεing at leaεt part of the region extending from amino acidε 105 to 110 characterized in that it: - either modulateε the lectin-like activitieε of TNF-α, - and/or inhibits the toxic activity of TNF-α, - and/or modulates the inflammatory cytokines inducing capacitieε of TNF-α, - and/or modulates the adheεion molecules inducing capacities of TNF-α, - and/or inhibitε the metaεtaεiε promoting activity of TNF-α, for the preparation of a medicament for treating either the TNF- induced εeptic εhock effect of TNF-α, or for treating pathological conditionε associated with the in vivo lectin-like effect of TNF-α, such as for treating εepsis, septic εhock, Gram negative εepsis, endotoxic shock, toxic shock syndrome, cachexia, microbial infectionε, rheumatoid arthritiε, inflammatory conditionε, reεpiratory diεtress syndrome, pulmonary fibrosis, infectionε, graft-versus-host-disease, reperfusion damage εuch as myocardial ischaemia, AIDS, cancer, immunoεuppression, cerebral malaria, etc. It iε to be underεtood that the antiεenεe peptideε according to the preεent invention are characterized in that they have at least one of the following effects: - they modulate (ie inhibit or εtimulate) , preferably inhibit, the lectin-like effect of TNF-α preferentially from 5 to 100%, preferably at leaεt 25%, more preferably at leaεt with 50%, most preferably with at least 75%; or, - they inhibit the toxic effect of TNF-α with from 5 to 50%, preferably at least 10%, more preferably with at least 25%, most preferably with at least 35%; or, - they modulate (i.e. inhibit or stimulate), preferably inhibit the inflammatory cytokines inducing capacities of TNF-α from 5 to 100%, preferably with at least 25%, more preferably with at least 50%, moεt preferably with at least 75%; or, - they modulate (i.e. inhibit or stimulate), preferably inhibit the adhesion molecules inducing capacities of TNF-α from 5 to 100%, preferably with at leaεt 25%, more preferably with at leaεt 50%, moεt preferably with at leaεt 75%; or, - they inhibit the metaεtaεiε promoting activity of TNF-α from 5 to 100%, preferably with at leaεt 25%, more preferably with at least 50%, most preferably with at leaεt 75% All theεe effectε may be meaεured as mentioned for the antibodies. More particularly, the term "antisense peptide" is reviewed by Blalock (1990) and by Rouboε (1990) . In thiε reεpect, the molecular recognition theory (Blalock, 1990) states that not only the complementary nucleic acid sequences interact but that, in addition, interacting siteε in proteinε are composed of complementary amino acid sequences (senεe ligand with receptor or εenεe ligand with antiεense peptides) . Thuε, two peptideε derived from complementary nucleic acid εequenceε in the εame reading frame will εhow a total interchange of their hydrophobic and hydrophilic amino acidε when the amino terminuε of one iε aligned with the carboxy terminuε of the other. Thiε inverted hydropathic pattern might allow two εuch peptideε to assume complementary conformations reεponεible for specific interaction. The antisenεe peptides can be prepared as deεcribed in Ghiεo et al. (1990) . By meanε of thiε technology it iε possible to logically construct a peptide having a physiologically relevant interaction with a known peptide by εimple nucleotide εequence analysis for complementarity, and synthesis of the peptide complementary to the binding site. The antisense peptides according of this preferred embodiment of the invention can be prepared by classical chemical syntheεiε. The εynthesis can be carried out in homogeneouε εolution or in solid phaεe. For inεtance, the synthesiε technique in homogeneouε εolution which can be uεed iε the one deεcribed by Houbenweyl in the book entitled "Methode der organiεchen chemie" (Method of organic chemistry) edited by E. Wunsh, vol. 15-1 et II. THIEME, Stuttgart, 1974. The antiεenεe peptides of the invention can also be prepared in solid phase according to the methods described by Atherton and Shepard in their book entitled "Solid phase peptide synthesiε" (IRL Preεε, Oxford, 1989) . In order to aεsume the inhibition of at least one, or preferably a combination of at least two of the afore mentioned effectε in vivo, the antisense peptide should form a complex with the subregion of the TNF-α tip region responεible for either of these effects of TNF-α. The present invention relates alεo to the uεe of a complex consisting of an antisense peptide as defined above, and TNF-α, for the preparation of a medicament. According to this embodiment of the invention, a pharmaceutical compoεition, containing, aε active εubεtance, a complex comprising an antiεenεe peptide as defined above, and complete TNF-α, in asεociation with a pharmaceutical acceptable vehicle, iε provided. Inεtead of, or in addition to TNF-α, the above mentioned compositions could also contain IL-lα, IL-lβ, IL-6, IFN-gamma, IFN α/β, IL-8,or any other immunoreactive agent. The above-mentioned compoεition can be uεed for treating illnesses wherein a treatment with TNF-α is beneficial such aε tumorε. According to a preferred embodiment, the present invention relates to the use of an antibody or an antisense peptide as defined above, characterized in that it modulates the lectin-like activities of TNF-α. According to an even more preferred embodiment, the preεent invention relateε to the uεe of an antibody or an antiεenεe peptide aε defined above, characterized in that it inhibitε the lectin-like activities of TNF-α. Illustrationε of said embodiment containing an antibody are given in the examples section of the invention. According to a possible embodiment, the present invention relates to the use of an antibody or an antisenεe peptide aε defined above, characterized in that it εtimulates the lectin- like activitieε of TNF-α. According to yet another preferred embodiment, the present invention relates to teh uεe of an antibody or an antiεenεe peptide aε defined above, characterized in that it inhibitε the toxic activity of TNF-α. According to yet another embodiment, the preεent invention relates to the uεe of an antibody or an antiεenεe peptide as defined above, characterized in that it modulates the inflammatory cytokineε inducing capacitieε of TNf-α. According to yet another preferred embodiment, the preεent invention relateε to the uεe of an antibody or an antiεense peptide as defined above, characterized in that it inhibits the inflammatory cytokineε inducing capacities of TNf-α. According to yet another embodiment, the present invention relates to the use of an antibody or an antiεenεe peptide aε defined above, characterized in that it modulateε the adheεion molecules inducing capacities of TNF-α. According to yet another preferred embodiment, the present invention relates to the use of an antibody or an antisense peptide as defined above, characterized in that it inhibits the adhesion molecules inducing capacitieε of TNF-α. According to yet another poεsible embodiment, the present invention relates to the use of an antibody or an antisenεe peptide aε defined above, characterized in that it εtimulates the adhesion molecules inducing capacities of TNF-α. According to yet another preferred embodiment, the present invention relate to the use of an antibody or an antisenεe peptide as defined above, characterized in that it inhibits the metaεtaεiε promoting activity of TNF-α, According to yet another preferred embodiment, the preεent invention relateε to the use of any of the above-defined antibodieε or antiεense peptideε, characterized in that they increase the half life time of TNF-α. Antibodies or antisense peptides according to thiε latter embodiment are preferably uεed in combination with TNF-α. It is to be understood that the TNF muteins according to the preεent invention can be adminiεtered either directly in the form of mutein protein in a pharmaceutical compoεition aε described above, or in the form of cellε tranεfected with the nucleic acid coding for εuch muteinε, εaid nucleic acid being inεerted into any εuitable vector, aε known to thoεe εkilled in the art. Thuε, the preεent invention relateε alεo to a method for treating patientε suffering from any of the above mentioned illnesεeε conεiεting of administering cells tranεfected with the cDNA coding for the TNF muteins as defined above, said nucleic acid being inserted into any suitable vector, as known to those skilled in the art. The cellε transfected by such a vector-nucleic acid combination are preferably autologous cellε derived from the patient (e.g. a cancer patient) to be treated with such compoεitionε. Said vector-nucleic acid combination has to be constructed in such a way aε to allow continuous expresεion of the mutein protein at either a conεtant level or at a level which can be modified, depending on the exact nature of the vector uεed to make the vector-nucleic acid combination. In this format, the present invention may be used to treat cancer patientε. ABBREVIATIONS TNF-α: tumor necroεiε factor-alpha mTNF- : mouεe tumor necroεis factor-alpha hTNF- : human tumor necrosis factor-alpha Bio: Biotin group r : recombinant wt : wild-type BRIEF DESCRIPTION OF THE FIGURES Figures IA and IB: Restriction map of the plasmid pIG2 used to expreεs the wild type and tip-mutated or tip-deleted mTNF-α. Figure 2: pIG2 nucleic acid sequence (SEQ ID NO 2) . The origin of nucleotide stretches used to construct plasmid pIG2 iε εpecified hereafter. Position 3300-206 : lambda PL containing EcoRI-MboII blunt fragment of pBL(λ) (Pharmacia) 207-266 : εynthethic εequence containing multiple cloning εiteε and the riboεome binding εite of which the ATG initiation codon iε located at position 232- 234 267-772 : rrnBT^ containing Hindlll-Sspl fragment from pKK223 (Pharmacia) 773-3300 : tetracycline resistance gene and origin of replication containing the EcoRI-Dral fragment of pAT 153 (Bioexcellence) Figureε 3A and 3B: In vi tro trypanocidal activity of recombinant mouεe (m) or human (h) TNF-α on T. brucei brucei (A) and T . rhodesiense (B) . Thereto, 2 x 10 6 /ml purified bloodstream trypanosomes, isolated 1 day before the first in vivo maximum peak, are incubated for 5 hours in PBS, 1% glucose, 1% normal mouse serum at 37 °C with various concentrations of recombinant TNF-α. After 5 hourε, the number of living paraεiteε iε aεεeεεed and iε compared with the control wells in which only incubation medium waε added. The spontaneous mortality in the control wells was alwayε lower than 10%. The valueε repreεent the meanε of quadruplicates and the standard deviation. Figure 4: LiCl potentiates the mouse TNF-α mediated trypanolysiε in vi tro . Trypanoεomes were incubated for 5 hours with εerial dilution of mTNF-α alone or enriched with 1 μg/ml of LiCl either in the preεence or abεence of anti-mTNF-α 1F3F3D4 monoclonal antibodieε, which are capable of neutralizing the cytotoxic effect of TNF-α. The valueε repreεent the meanε of quadruplicateε and the εtandard deviatation. Eεpecially at low TNF-α concentrations (50 and 500 pg/ml) , the valueε in the LiCl group differed significantly (<0.0008 and 0.009) from the control group indicating that LiCl synergiεtically potentiates the trypanocidal effect of mTNF-α. LiCl by itself had no effect on the survival of the trypanosomes as no difference with control values were seen (data not εhown) . Figures 5A and 5B: T. brucei brucei lysate inhibits the in vi tro trypanocidal but not the cytolytic (cytotoxic) capacity of TNF- α. Preincubation of mTNF-α for 1 h with 10 μg/ml of trypanosome lysate results in a potent inhibition of the TNF-α-εpecific trypanocidal effect (A) , whereaε it haε no effect on the cytolytic or tumoricidal capacity of TNF-α (B) . The neutralizing anti-mTNF-α 1F3F3D4 monoclonal antibody (anti-mTNF; Lucaε et al. , 1990) waε used to demonstrate that cytolysis waε εpecifically indicated by TNF-α. The values represent the means of quadruplicates and the standard deviation. Figures 6A and 6B: Preincubation of mTNF-α with N,N'- diacetylchitobioεe (indicated aε chitobioεe) potently inhibits the trypanocidal activity (A) , but not the cytolytic activity (B) of mouse TNF-α. mTNF-α was preincubated for 2 h with 1 μg/ml of N,N' -diacetylchitobiose before adding it in a serial dilution to bloodsteam formε of T. brucei brucei (A) or to L929 cellε (B) to be teεted on itε trypanocidal or cytolytic activity (as described in the examples) , respectively. Figure 7: Trypanocidal activity of the circularized, biotinylated mouse TNF-α tip peptide (Bio- GG-CGPKDTPEGAELKPWYC) (mtip) and the biotinylated human TNF-α tip peptide (Bio-GG- CGQRETPEGAEAKPWYC) (htip) , and the biotinylated linear subpepl (Bio-GG-CTPGAEC) (εubtip) on the bloodεtream forms of T. brucei brucei and inhibition of the trypanocidal activity by pre¬ incubation with N,N' -diacetylchitobiose (Chitobiose) . Serial dilutions of theεe circularized mouεe or human TNF-α tip peptideε were added either directly to 2 x 10 6 /ml purified bloodstream trypanosomeε or after pre-incubation for 2 h with 1 μg/ml of N,N' -diacetylchitobiose. After 5 hours, the number of living paraεiteε iε aεsesεed and iε compared with the control wells in which only incubation medium waε added. Figure 8: Trypanocidal activity of different εynthetic tip peptideε. Serial dilutionε of mTNF-α tip (Bio-GG- CGPKDTPEGAELKPWYC) , hTNF-αtip (Bio-GG-CGQRETPEGAEAKPWYC) , εubpep 2 (Bio-GG-EGAELKPWY) , εubpep 1 (Bio-GG-C-TPEGAE-C) and the D- amino acid analogue of εubpep 1 (=subpep 4) were added to 2 x lOV l purified bloodstream forms of T. brucei brucei . Figure 9: Trypanocidal activity of εubpeptide 1 and muteins thereof. Serial dilutions of subpep 1 (Bio-GG-C-TPEGAE-C) , subpep 3 (Bio-GG-TPE) , mutpep 1 (Bio-GG-C-APEGAE-C) , mutpep 2 (Bio-GG-C- TAEGAE-C) , mutpep 3 (Bio-GG-C-TPAGAE-C) , mutpep 4 (Bio-GG-C- TPEAAE-C) and mutpep 5 (Bio-GG-C-TPEGAA-C) made in 100 μl were added to 2 x 10 5 /ml purified bloodεtream forms of T. brucei brucei (final concentration IO 6 parasites/ml) . Aε in Figure 8, after 5 hourε, the number of live paraεiteε is asεeεsed and compared with the control wells in which only incubation medium iε added. Figure 10: The anti-mTNF-α tip polyclonal antibody (anti-tip) potently inhibitε the trypanocidal effect of mTNF-α, but it only weakly inhibitε the cytolytical activity againεt L929 cellε. mTNF-α waε preincubated for 1 h with 10 μg/ml of protein G purified anti-tip polyclonal IgG before adding it to 2 x IO 6 bloodεtream purified T. brucei brucei /ml . After 5 hourε the trypanocidal activity of mTNF-α alone or pretreated with anti-tip polyclonal IgG waε evaluated by counting the number of live parasites. The cytolytical activity of TNF-α alone or pretreated with anti-tip polyclonal IgG was evaluated on cell εurvival of L929 cellε after 48 hours incubation in the absence of actinomycin D. Figure 11: In vi tro cytolytic (cytotoxic) activity of wild type mTNF-α and mTNFT105A on L929 cells aε teεted in the 48 hourε assay in the absence of Actinomycin D. The assay was performed as explained in the Materialε and Methodε εection of the Exampleε. Figure 12: In vi tro T. brucei brucei trypanocidal of wild type mTNF-α and T105A mTNF-α. The trypanocidal activity of wild type and T105A mutated mTNF-α was tested aε explained in the legend of figure 3. Figure 13 : Effect of the intravenouε (i.v.) injection of different concentrations of wild type and T105A mutated mTNF-α on the εurvival time of C57BL/6 mice. Per group of 6 mice, 10 μg top left) , 5 μg (top right) , 2.5 μg (bottom left) or 1 μg (bottom right) per 200 μl of PBS of either wild type mTNF-α or T105A tip mutated mTNF-α was i.v. injected and the survival time was asεeεεed until 72 hourε after injection. EXAMPLES Materials and methods 1. Animalε Female 10-week old Balb/C mice (SCK, Mol, Belgium) were uεed in all in vivo LPS-induced εeptic shock experiments. Female 10-weeks old C57BL/6 mice (Iffa Credo, France) were used in all in vivo TNF-induced lethal shock experiments. 2. Cell lines L929 fibroεarcoma cellε were obtained from the Rega Inεtitute, Leuven, Belgium. 3. Trypanosomes The Trypanosoma brucei brucei AnTat 1.1 (EATRO 1125) pleomorphic bloodstream form was kindly provided to uε by Dr. N. Van Meirvenne of the Inεtitute for Tropical Medecine, Antwerp, Belgium, and the Trγpanosoma brucei rhodesiense Trp 11 pleomorphic bloodstream form was kindly provided to uε by Dr. E. Bajyana Songa (Dept. Molecular Biology, Free Univerεity of Brusεelε, Belgium) A T. brucei lysate was prepared by a triple freeze-thaw cycle of purified trypanosomes. 4. Cytokineε, antibodieε and biochemicalε Purified recombinant mTNF-α and recombinant hTNF-α, produced in E. coli . with a εpecific activity of 10 s units/mg and 2 x 10 7 units/mg reεpectively, were produced by Innogeneticε, Gent, Belgium. Human recombinant TNF-β (lymphotoxm) iε commercially available from the Genzyme Corporation (Cambridge, MA, USA) . The rat-anti-recombinant mTNF-α 1F3F3D4 (Lucas et al. , 1990) and the mouse-anti-recombinant hTNF-α monoclonal antibodies are both commercially available from Innogenetics (Ghent, Belgium) . The soluble p55 hTNF-α receptor protein waε obtained from Dr. D. Wallach (Dept. of Membrane Reεearch &. Biophysics, Weizmann Institute, Rehevot, Israel) . Lipopolysaccharide W E . col i 055:B5 (LPS) was purchased from Difco Laboratories, Detroit, MI, USA. 5. Oligoεaccharides/lectins N,N' -diacetylchitobioεe waε purchaεed from Sigma (St. Louiε, MO, USA) . In the in vi tro TNF-α-trypanocidal aεεay, the TNF-α-derived tip peptideε are preincubated for 1 h with l μg/ml oligosaccharide. The lectins Griffonia (Bandeiraea) simplici folia A4 (GSI-A4) and Tri ticum vulcraris agglutinin (WGA) were purchased from Sigma (St. Louiε, MO, USA) . Galanthus nivaliε agglutinin (GNA) and Urtica dioica agglutinin (UDA) were purified and kindly provided by Dr. L. Wijnε (Laboratory of Cryεtallography, Free University of Brusεels, Belgium) . 6. In vi tro biological asεay to meaεure the cytotoxic and trypanocidal activity of recombinant hTNF-α or mTNF-α 6.1. L929 cell killing assay TNF-sensitive mouεe fibrocarcinoma L929 cellε in complete RPMI 1640 medium (Gibco BRL, Paisley, Scotland) were treated with actinomycin D (1 μg/ml) at a cell concentration of 3 x 10 s cells/ml. Thuε, 100 μl of the εuspension in 96-well flat bottomed culture plates was mixed with 100 μl, containing either medium (negative control) , 100 μl h/mTNF-α (serially diluted in medium; poεitive control) , or 100 μl h/mTNF-α + 1 μg/ml of oligoεaccharide, or 100 μl of the TNF-α derived tip peptideε (εerially diluted in medium) . After 18 h of culture at 37°C, viability of the cell cultures was assesεed by dye uptake analyεiε. The latter waε accomplished by decanting the medium from the plates and εtaining the cellε for 10 min with Crystal Violet (0.5% Crystal Violet, 8% (v/v) formaldehyde (40%), 0.17% NaCl, 22.3% (v/v) ethanol). After staining, the cells were extenεively waεhed with tap water and the dye waε dissolved in 100 μl/well of 33% acetic acid. The plates were read spectrophotometrically at 577 nm with a Titertek multiscan MCC340 ELISA Reader (Flow Laboratories, McLean, VA) . Sometimeε, the cytotoxic aεεay waε performed in the abεence of actinomycin D using an identical experimental set-up but a longer incubation period (48 instead of 18 hours) . The latter asεay is leεs sensitive but measures the intrinsic cytotoxic activity of the TNF-α protein. 6.2. In vi tro trypanocidal assay Two times 10 s /ml purified bloodstream forms of Trγpanoεoma brucei brucei or T. brucei rhodesiense iεolated 1 day before the firεt in vivo maximum peak iε reached, were incubated for 5 h in phoεphate buffered εaline (PBS) , 1% glucose, 1% normal mouse serum (incubation medium) at 37°C with variouε concentrations of recombinant mTNF-α, recombinant hTNF-α or the TNF-α-derived tip peptides, all in the absence or the preεence of anti-tip peptide polyclonal or monoclonal antibodies. After 5 h of incubation, the number of live (= moving) parasiteε waε assessed (counting chamber) and compared the control wells in which only incubation medium was added to the trypanoεomeε. The εpontaneouε mortality in the control wells after 5 h was always lower than 10%. All treatments were done in quadruplicate and all reported in vitro trypanolyεiε experiments were repeated at least 3 times. 7. In vivo assay to evaluate the tumoricidal effect of human and mouse TNF-α 7.1. Anti-tumor effect of hTNF-α on εubcutaneous HT-29 tumors in nude mice. To test the anti-tumor effect of wild type and tip-mutated or -deleted hTNF-α, 5 x IO 6 HT-29 human adenocarcinoma (ATCC HTB38) were subcutaneouεly (ε.c.) injected in nude mice (Iffa Credo, France) . The treatement compriεeε daily perileεional injectionε for 6 dayε per week, followed by 1 day without a treatement thiε for a period of 1 month. Perileεional is a subcutaneous injection near the site of the tumor but outside the tumor nodule. Per mouse, 5 μg of human recombinant TNF-α waε injected. Tumor volume was estimated every 3 or 4 days by measuring the larger (a) and the smaller (b) diameter and calculating the a x b 2 x 0.4 as known in the art. 7.2. The B16B16 melanoma model to test the anti-tumor activity of wild type and tip-mutated or tip-deleted mTNF-α. 5.10 s B16B16 melanoma cells (Hart and Fidler, 1980) are injected subcutaneouεly (ε.c.) in the back of C57/black mouse (Iffa Credo, France) . Between days 8 and 10, a subcutanous tumor nodule appearε at the inoculum εite in all animalε. Treatment εtarted at day 10 for 10 dayε and conεiεted of a daily perileεional injection of 5 μg mTNF-α/mouεe. The tumor volume waε eεtimated aε mentionned above. 8. In vivo aεεayε to evaluate the toxic effect or septic shock effect of recombinant hTNF-α or mTNF-α 8.1. Induction of the LPS-induced septic εhock syndrome in mice Intraperitoneal (i.p.) injection of 500 μg of LPS/mouse causes death within 48 hours. Protection against εeptic εhock εyndrome waε aεsayed by injecting 10-weekε old Balb/C mice (10 mice/group) with 100 μg of the protein G-purified rabbit-anti-mTNF-α-tip peptide polyclonal antibodieε in 200 μl PBS, or with an equal amount of control rabbit polyclonal antibodieε or with 200 μl PBS, 2 h before injection of 500 μg of LPS/mouse. The number of days of postinjection survival was then asεeεεed. 8.2. Induction of the TNF-α-induced lethal shock Intravenous (i.v.) injection of 10-week-old C57BL/6 mice with 10 μg/mouse of mTNF-α causeε death within 48 hourε. Ten weeks old C57BL/6 mice (5 to 6/group) were injected with different doseε of wild type or mutant mouεe TNF-α and the poεtinjection survival time was then asεessed. 9. Statistics All data were analyzed using the Mann-Whitney U teεt. 10. Biotinylated peptide εynthesis All amino acid εequenceε are given in the conventional and universally accepted three-letter or one-letter code. The peptide sequences are given left to right which, by convention, is the direction from the amino terminuε to the carboxyterminus. A number of unconventional codes are also used to represent chemical groupε or modificationε and are defined aε followε: Ac = acetyl group Bio = D-biotinyl group Fmoc = 9-fluorenylmethoxycarbonyl group tBoc = tertiary butyloxycarbonyl group All of the peptideε deεcribed were εynthesized on TentaGel S-RAM (Rapp Polymere, Tubingen, Germany) , a polystyrene -polyoxyethylene graft copolymer functionalized with the acid-labile linker 4- (α-Fmoc-amino-2' ,4' -dimethoxybenzyl) phenoxyacetic acid (Rink et al. , 1987) in order to generate peptide carboxy-terminal amides upon cleavage, t-Butyl-based side chain protection and Fmoc-α-amino-protection was uεed. The εulfhydryl group of cysteine was protected with a trityl group. Couplings were carried out using preformed O-pentafluorophenyl esterε. Biotin waε coupled using TBTU as the activating agent in the presence of two equivalents of the base N-methylmorpholine. All syntheseε were carried out on a Milligen 9050 PepSynthesizer (Novato, CA) using continuous flow procedures. Following cleavage with trifluoroacetic acid in the presence of scavengers and extraction with diethylether, all peptides were analyzed by Cl8-reverse phase chromatography. 11. ELISA assay to quantify wild type and tip-mutated or tip-deleted mouse TNF-α protein An ELISA aεεay to measure the protein concentration of the wild type and tip-deleted or tip-mutated mouse TNF-α protein waε developed. Thereto, the microtiter plateε were coated overnight at room temperature with 100 μl per well of 2.5 μg/ml of rabbit anti-mouεe TNF-α (Innogeneticε, Belgium) in 10 mM Triε, 10 mM NaCl, 10 mM NaN3. Subεequently, the plateε were waεhed once with waεhing buffer (PBS, 0.05 % Tween-20) and the remaining binding εiteε were blocked for at leaεt one hour at 37 °C with 300 μl per well of PBS buffer supplemented with 1 % BSA and 0.1 % NaN3. Dilutions of sample or standard mTNF-α (0.01 to 10 ng/ml mTNF-α) , made in 100 μl of PBS supplemented with 1 % BSA, 1 % rabbit serum, and 0.1 % mertiolate are then added to each well and incubated for 2 hourε at 37 °C. Thereafter, the plates are washed 4 times with waεhing buffer and 100 μl/well of biotinylated rabbit anti-mouεe TNF-α which haε been diluted with εample diluent to a concentration of 0.125 μg/ml iε added for another 2 hourε at 37 °C. Subεequently, the plateε are waεhed four times with washing buffer wherafter 100 μl of straptavidin-horεeradish peroxidase (Jackεon Immunoresearch Labs) , which has been diluted in εample diluent to a concentration of 100 ng/ml is added and incubated for 30 minutes at 37 °C. To each well, 100 μl of TMB solution (8.7 g/1 tetramethyl benzidine in DMSO) which has been diluted 100 times in εubεtrate buffer (εolution A: 0.1 M Na2HP4.2H20; εolution B: 0.1 M citric acid; εolution C: 3 % H202. Mix 149 partε of εolution A with 100 partε of εolution B, and add 2 ml of solution C per 1 liter mix) is added and incubated for 30 minuteε at room temperature. The reaction iε εtopped by adding 100 μl per well of 2 N H2S04 wherafter the optical denεity iε read at dual wavelength (450/595 nm) . EXAMPLE 1; Generation of recombinant wild-type and tip- mutated or -deleted mTNF-α protein in E. coli A schematic overview of the amino acid εequence spanning the region of amino acidε 105 to 110 of mTNF-α and all conεtructed mTNF-α muteinε iε given in Table II. Table II Positions 105 106 107 108 109 110 mTNF-α Thr Pro Glu Gly Ala Glu T105A mTNF Ala Pro Glu Gly Ala Glu P106A mTNF Thr Ala Glu Gly Ala Glu E107A mTNF Thr Pro Ala Gly Ala • Glu E110A mTNF Thr Pro Glu Gly Ala Ala E107A / E110A mTNF Thr Pro Ala Gly Ala Ala T105A / E110A mTNF Ala Pro Glu Gly Ala Ala T105A / E107A mTNF Ala Pro Ala Gly Ala Glu T105A / E107A / E110A mTNF Ala Pro Ala Gly Ala Ala del TPEGAE mTNF - - - - - - 1. Construction of pIG2mTNF A 701 bp Taql/Xbal fragment, containing the mTNF-α coding sequence except the εignal εequence and the firεt 21 bp of the mature εequence, waε isolated from pBSK(+)mTNF. This pBSK(+)mTNF vector contains the full-size mTNF-α cDNA as been published by Fransen et al, (1985) . This fragment was subsequently ligated to the following synthetic DNA εequence : 5 ■ -CATGCTCAGATCAAGTAGTCAAAATT-3 ' (SEQ ID NO 3) 3' -GTACGAGTCTAGTTCATCAGTTTTAAGC-5' (SEQ ID NO 4) L εtartcodon mature mTNF-α This new 727 bp sequence was then ligated into the Sspl/Xbal opened vector pIG2 (SEQ ID NO 2) , resulting in the vector pIG2mTNF. pIG2 is a versatile expression vector for E. coli . It contains the temperature-inducible leftward promotor of phage lambda, a εynthetic riboεome binding site, a multilinker sequence, the rrnBTlT2 tranεcriptional terminator and a tetracycline reεiεtance gene (Fig. 1 and 2) Tranεformation and εubεequent induction of pIG2mTNF to E. coli host MC1061(pAcI) resulted in an expression level of ± 10% of total cellular protein (data not shown) . 2. Conεtruction of mTNF-α Thrl05 mutant pIG2mTNFT105A The mTNF-α Thrl05 mutant, wherein the Thrl05 waε replaced by an Ala reεidue, was generated by introducing the synthetic oligonucleotide primers 5'- CAAGGACGCTCCGGAGGGGGCTGAGCT-3' and 5'- CAGCCCCCTCCGGAGCGTC-3 ' in the Styl - Sad digested wild type (wt) mTNF-α, thereby replacing the Thrl05 by an Ala residue and creating an unique BεtEI control reεtriction εite in the mutated TNF insert, which iε not present in the wt TNF-α sequence. Thereto pIG2mTNF DNA (13 μl = ± 8 μg) was cut with BamHI (40 Units, 10 Units/μl, Boehringer Mannheim) in a total volume of 80 μl buffer B (Boehringer Mannheim) for 3 hours at 37°C. The digeεted DNA waε purified via the Geneclean method (BiolOl, La Jolla) and reεuspended in 68 μl of sterile doublediεtilled water. To thiε volume 8 μl of 10 timeε concentrated buffer H (Boehringer Mannheim) and 40 Unitε Styl (Boehringer Mannheim, 10 Unitε/μl) )were added and again incubated for 3 hourε at 37°C. A 911 bp BamHI/Styl fragment waε iεolated from a 1.2% agaroεe gel via the Geneclean method and reεuspended in 20 μl sterile doublediεtilled water. This material is called fragment 1. To generate the second fragment, pIG2mTNF DNA (8 μl = ± 5 μg) waε cut with BamHI (40 U, 10 U/μl, Boehringer Mannheim) in a total volume of 80 μl buffer B (Boehringer Mannheim) for 3 hours at 37°C. The digested DNA waε purified via the Geneclean method (BiolOl, La Jolla) and reεuεpended in 68 μl of sterile doubledistilled water. To this volume 8 μl of 10 timeε concentrated buffer A (Boehringer Mannheim) and 40 Units Sad (Boehringer Mannheim, lOU/μl) were added and again incubated for 3 hourε at 37°C. A 3071 bp SacI/BamHII fragment waε isolated from a 1.2% agarose gel via the gene clean method and reεuεpended in 40 μl sterile doubledistilled water. This material iε called fragment 2. To generate the mTNF-α Thrl05 mutant pIG2mTNFT105A, 400 pmole (100 pmole/μl) of each of the εynthetic oligoε in a total volume of 10 μl were heated for 3' at 95°C , 10' at 65°C and were εubsequently allowed to reach roomtemperature. The annealed oligos were subsequently placed in ice. 5' -CAAGGACGCTCCGGAGGGGGCTGAGCT-3' 27-mer (SEQ ID NO 5) 3'-CTGCGAGGCCTCCCCCGAC-5' 19-mer (SEQ ID NO 6) BspEI Fragment 1 (5 μl) , fragment 2 (1.5μl) and the annealed oligos (10 μl) were mixed in a total volume of 19 μl ligation buffer (Boehringer Mannheim) , 1 Units of T4 ligase (Boehringer Mannheim, 1 Unit/μl) was added and the mixture was incubated for 3 hours at 15°C. The ligation mixture was tranεformed to DH1(lambda) competent cellε, plated out on Tetracyclin containing LB plates and grown overnight at 37°C. Plasmid DNA was prepared from εelected colonies and analyεed via reεtriction digeεtion (BspEI, BamHI, Styl and Sad) . A via restriction analyεiε confirmed vector pIG2mTNFT105A waε tranεformed to MC1061 (pAcI) . This vector/host combination waε further uεed for prokaryotic expression of the mTNFT105A mutein. 3. Construction of mTNF-α Prol06 mutant pIG2mTNFP106A In this conεtruct the Prol06 of mTNF-α iε changed to an Ala reεidue. In the course of the construction an extra Narl was created in the mTNF coding sequence, which iε not present in the wt mTNF sequence. Thereto pIG2mTNF DNA (13 μl = ± 8 μg) waε cut with BamHI (40 Units, 10 Units/μl, Boehringer Mannheim) in a total volume of 80 μl buffer B (Boehringer Mannheim) for 3 hourε at 37°C. The digeεted DNA waε purified via the Geneclean method (BiolOl, La Jolla) and reεuεpended in 68 μl of sterile doubledistilled water. To thiε volume 8 μl of 10 timeε concentrated buffer H (Boehringer Mannheim) and 40 Unitε Styl (Boehringer Mannheim, lOU/μl) were added and again incubated for 3 hourε at 37°C. A 911 bp BamHI/Styl fragment was isolated from a 1.2% agaroεe gel via the Geneclean method and reεuεpended in 20 μl sterile doubledistilled water. This material waε called fragment 3. To generate the εecond fragment, pIG2mTNF DNA (8 μl = ± 5 μg) was cut with BamHI (40 Units, 10 Units/μl, Boehringer Mannheim) in a total volume of 80 μl buffer B (Boehringer Mannheim) for 3 hours at 37°C. The digeεted DNA waε purified via the Geneclean method (BiolOl, La Jolla) and reεuεpended in 68 μl of εterile doublediεtilled water. To thiε volume 8 μl of 10 timeε concentrated buffer A (Boehringer Mannheim) and 40 Unitε Sad (Boehringer Mannheim, lOU/μl) were added and again incubated for 3 hourε at 37°C. A 3071 bp SacI/BamHII fragment waε iεolated from a 1.2% agarose gel via the Geneclean method and resuspended in 40 μl sterile doubledistilled water. This material was called fragment 4. To generate the mTNF-α Prol06 mutant pIG2mTNFP106A, 400 pmole (100 pmole/μl) of each of the synthetic oligos in a total volume of 10 μl were heated for 3' at 95°C, 10' at 65°C and were subεequently allowed to reach room temperature. The annealed oligos were subεequently placed on ice. 5'-CAAGGACACCGCTGAGGGCGCCGAGCT-3' 27-mer (SEQ ID NO 7) 3'-CTGTGGCGACTCCCGCGGC-5' 19-mer (SEQ ID NO 8) I I Narl Fragment 3 (5 μl) , fragment 4 (1.5 μl) , and the annealed oligos (10 μl) were mixed in a total volume of 19 μl ligation buffer (Boehringer Mannheim) . One Unit of T4 ligase (Boehringer Mannheim, 1 U/μl) waε added and the mixture was incubated for 3 hourε at 15°C. The ligation mixture waε transformed to DH1 (lambda) competent cells, plated out on tetracyclin containing LB plates and incubated at 37°C. Plasmid DNA was prepared from εelected colonieε and analyzed via reεtriction digestion (Narl, BamHI, Styl and Sad) . A vector confirmed via restrication analyεiε (pIG2mTNFP106A) waε transformed to MC1061 (pAd) . This vector/host combination waε further uεed for expression of the mTNFP106A mutein. 4. Construction of other tip mutated mTNF-α 4.1. Construction of pIG2mTNFE107A In thiε construct, the Glul07 residue of mTNF was replaced with an Ala residue. In the course of the construction, a unique Pstl restriction site was created in the mTNF coding sequence, which is not present in the wt mTNF sequence. Construction of the vector was similar to the previously constructed mutants with the following differences: fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3071 bp SacI/BamHI fragment from pIG2mTNF oligos K D T P A G A E 5' -CAAGGACACCCCTGCAGGCGCTGAGCT-3' 27-mer(SEQ ID NO 9) 3' -CTGTGGGGACGTCCGCGAC-5' 19-mer(SEQ ID NO 10) I I Pstl 4.2. Construction of pIG2mTNFE110A mutant In this construct, the GlullO residue of mTNF was replaced with an Ala residue. In the course of the construction, a unique Narl reεtriction site was created in the mTNF coding εequence, which iε not preεent in the wt mTNF εequence. Moreover, the unique Sad restriction site preεent in the wt mTNF εequence waε deεtroyed. Construction of the vector was similar to the previous conεtructed mutants with the following differences : fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3071 bp Sad blunt/BamHI fragment from pIG2mTNF Blunting of the Sad site was done by cutting the pIG2mTNF vector with Sad, purifying the linear DNA via the gene clean method (resuspended in 20 μl distilled water) . DNA in 20 μl water + 3 μl of a 500 mM Triε-Cl pH 7.4, 5 mM MgC12, 2 ' mM β-Me EtOH solution + 1 μl dXTP solution 5 mM each + 2 μl T4 polymerase ( lU/μl) + 4 μl doubledistilled water The mixture specified above was incubated for 60 min at 37°C followed by the purification of the blunted DNA via the gene clean procedure and digestion of the DNA with BamHI. oligos K D T P E G A A ■ CAAGGACACCCCTGAGGGCGCCGCCCT-3 ' 27 -mer ( SEQ ID NO 11) 3 ' -CTGTGGGGACTCCCGCGGCGGGA-5 ' 23 -mer (SEQ ID NO 12 ) Narl 4.3. Conεtruction of pIG2mTNFE107A/E110A In thiε construct, the Glul07 and GlullO residueε of mTNF were replaced with Ala reεidueε. In the courεe of the conεtruction unique Narl and Pεtl restriction sites were created in the mTNF coding sequence, which were not present in the wt mTNF sequence. Moreover, the unique Sad restriction site present in the wt mTNF sequence was destroyed. Construction of the vector was similar to the previouε conεtructed mutants with the following differences : fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3071 bp Sad blunt/BamHI fragment from pIG2mTNF oligos K D T P A G A A 5' -CAAGGACACCCCTGCAGGCGCCGCCCT-3' 27-mer(SEQIDNO13) 3' -CTGTGGGGACGTCCGCGGCGGGA-5' 23-mer(SEQIDNO14) Narl Pεtl 4.4. Construction of pIG2mTNFT105A/E110A In thiε conεtruct, the Thrl05 and GlullO reεidues of mTNF were replaced with Ala residueε. In the courεe of the conεtruction unique Narl and BεpEI reεtriction εiteε were created in the mTNF coding εequence, which were not preεent in the wt mTNF εequence. Moreover, the unique Sa reεtriction εite preεent in the wt mTNF sequence was destroyed. Construction of the vector was εimilar to the previouε conεtructed mutantε with the following differences : fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3071 bp Sad blunt/BamHI fragment from pIG2mTNF oligos K D A P E G A A 5' -CAAGGACGCTCCGGAGGGCGCCGCCCT-3' 27-mer(SEQIDNO15) 3' -CTGCGAGGCCTCCCGCGGCGGGA-5' 23-mer(SEQIDNO16) BspEI Narl 4.5. Construction of pIG2mTNFT105A/E107A In this construct, the Thrl05 and Glul07 reεidueε of mTNF were replaced with Ala reεidueε. In the courεe of the conεtruction unique Neil and Bsgl restriction εites were created in the mTNF coding sequence, which were not present in the wt mTNF sequence. Construction of the vector was similar to the previous constructed mutants with the following differences : fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3071 bp SacI/BamHI fragment from pIG2mTNF oligos K D A P A G A E 5' -CAAGGATGCCCCGGCGGGTGCAGAGCT-3' 27-mer(SEQIDNO17) 3' -CTACGGGGCCGCCCACGTC-5' 19-mer(SEQ ID NO 18) I I I I Neil Bεgl 4.6. Construction of pIG2mTNFT105A/E107A/E110A In this construct, the Thrl05, Glul07 and GlullO residueε of • mTNF were replaced with Ala residues. In the course of the construction, unique Eagl and Sfil restriction sites were created in the mTNF coding εequence, which were not preεent in the wt mTNF εequence. Moreover, the unique Sad reεtrictionεite present in the wt mTNF sequence was deεtroyed. Conεtruction of the vector was similar to the previouεly conεtructed mutants with the following differences: fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3071 bp Sad blunt/BamHI fragment from pIG2mTNF oligos K D A P A G A A -CAAGGATGCTCCGGCCGGTGCGGCCCT-3' 27-mer(SEQIDNO19) 3' -CTACGAGGCCGGCCACGCCGGGA-5' 23-mer(SEQIDNO20) Sfil Eagl 5. Construction of the tip-deleted mTNF-α or pIG2mTNF TPEGAE In this construct, the TPEGAE coding sequence iε deleted from the mTNF gene. In the courεe of the conεtruction a unique Ncol reεtriction εite waε created in the mTNF coding εequence, which is not present in the wt mTNF sequence. Construction of the vector was similar to the previously conεtructed mutants with the following differences : fragment 1 = 911 bp BamHI/Styl fragment from pIG2mTNF fragment 2 = 3046 bp BεtXI/BamHI fragment from pIG2mTNF oligoε K D L K P W Y E P I Y 5 ' -CAAGGACCTCAAACCATGGTATGAGCCCATATAC-3 ' 34-mer (SEQ ID NO 21) 3 ' - CTGGAGTTTGGTACCATACTCGGGTA- 5 ' 26 -mer ( SEQ ID NO 22 ) Ncol The conεtruction of additional tip-region deleted mTNF-α mutantε alεo lies within the object of the present invention. These additional mutants are to be constructed uεing techniqueε known in the art aε deεcribed above. 6. E. coli expreεsion of wild type and tip-mutated or deleted mTNF-α. Batch fermentations were conducted in LB medium (0.5% yeast extract, 1% bactotryptone, 0.5% NaCl), at a 15-liter scale, with a minimum dissolved oxygen content of 50%. pH waε maintained constant during the whole fermentation at a value of 6.9. Foaming problems were avoided by adding 0.013% polypropylene glycol P2000. Precultures of the different vector/host combinations were grown overnight in LB medium εupplemented with tetracycline 0,001% for 18 hourε at 28°C. After inoculation of the medium with a final OD 6E0 of 0.05, the bacteria were εubεequently grown for 4 generationε at 28°C, and for 4 hourε at 42°C. At the end of the fermentation, the cells were separated from the medium by centrifugation, suspended in lysis buffer (10 mM KC1, 10 mM Tris- HCl, pH 6.8, 5 mM EDTA) and stored at -70°C. 7. Purification of recombinant wild-type and tip-mutated or deleted mTNF-α. 7.1 Purification of wild type mouse TNF-α To the bacterial cell suspension, aminocapronic acid, PMSF and DTT are added to a final concentration of respectively 25 mM, 1 mM and 1 mM before lyεing by French Press (14,000 psi, AMINCO, SLM Inεtrumentε, Urbana, USA) . Subεequently, the εuεpension is centrifugated for 20 minutes at 16,500 rpm at 4°C using a JA-20 rotor (Beckman) . Wild-type mTNF-α is found in the supernatant fraction as assessed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) . Some of the TNF-α tip muteins were retrieved in the soluble and insoluble fraction. The described procedure is directed to the recovery of the mTNF-α material out of the soluble fraction. No attemps were made to purify and renaturate mTNF from the insoluble fraction. The nucleic acids in the supernatant were precipitated at pH 7.0 by the addition of polyethylene-imine to a final concentration of 0.4% followed by centrifugation by a JA-20 rotor for 20 minutes at 16,500 rpm at 4°C. mTNF-α or mutein protein were then enriched by a differential amoniumsulphate precipitation whereby a 40% εtep iε followed by a εecond εtep at 65% saturation. mTNF-α or muteins remained in the 65% amonium sulphate pellet. The pellet was resuspended in 25 mM Tris-HCl pH 7.2 and the conductivity of the solution was adjusted to 10 mS above the conductivity of the Phenyl εepharose column startbuffer (25 mM TrisHCl pH 6.9, 25% (NH4)2S04). The material waε then loaded at 0.5 cm/minute on the Phenyl Sepharoεe 4B matrix (Pharmacia) and waεhed with 8 column volumeε of starting buffer. Proteins bound to the matrix were eluted with a linear εalt gradient of 0% to 60% buffer B (20 mM ethanolamine, 10% ethylene glycol pH 9.0) over 6 column volumes. The flow through fractions containing the mTNF-α protein were pooled and dialysed overnight against 25 mM Tris-HCl pH 7.2. After centrifugation, the material was loaded on Q Sepharoεe FF (Pharmacia) equilibrated againεt 25 mM Tris-HCl pH 7.2 at 1 cm/minute. The column was washed with 4 column volumes of starting buffer and eluted with a linear εalt gradient form 0 mM to 300 mM NaCl in the εame buffer followed by εtepwise elution with 2 column volumeε at 1 mM NaCl. Recombinant mTNF-α eluted at 300 mM NaCl. By this procedure 98% pure mTNF-α could be obtained with a molecular maεε of 17 kDa, aε defined by SDS-PAGE under reducing conditionε, and a εpecific activity of ± 2 x IO 8 U/mg as asseεεed by bioactivity on L929 cellε. 7.2. Purification of P106A mTNFα mutein Cell lyεiε, clearing and polyethylene imine treatment were performed aε described in 7.1. In contrast to the physico chemical behaviour of wt mTNF, the P106A mutein was recovered in the PEI pellet. Therefore, the pellet waε extracted with 20 mM Triε-Hcl, pH-7.0, 0,5 MNaCl (200 ml/pellet) and cleared by centrifugation (JA 20 rotor, 16 500 rpm, 20 min, 4°C) . The purification proceεs ( (NH4)2S04 fractionation and chromatography stepε on Phenyl Sepharoεe 4B and Q Sepharose FF) were identical to these described in the section 7.1 as for wt mTNF-α. Over 95% pure P106A mTNF-α was obtained by thiε procedure with a εpecific activity of ±1.81 x 108 U/mg as asseεsed by bioassay on L-929 cells in the presence of Actinomycin D. 7.3. Purification of the T105A mTNFα mutein Cell lyεiε, clearing and PEI treatment of the εupernatanε were performed aε described in for wild type TNF-α. As for the P106A mutein, the T105A mTNF-α waε recovered in the PEI pellet. Extraction and clearing were therefore performed aε already deεcribed in εection 7.2 for the P106A mutein. The conductivity of the εupernatant waε adjusted to 10 mS above this of the equilibration buffer of the Phenyl Sepharose 4B column. The chromatographical conditions were identical to those described in section 7.1, except that an equivalent of a 5 liter cell culture was loaded on a 40 ml column. The T105A mTNF-α mutein was recovered in .the first part of the gradient (about 0.9 M → 0,5 M (NH4)2S04). The pools of the 3 runs on Phenyl Sepharose 4B were mixed and after adjustment of the pH and the conductivity, the total pool was loaded on a 16 ml Phenyl Q Sepharose FF column. Chromatography conditions (flow rate (cm/h) and slope of the gradient) were kept identical as for these used on the previous Phenyl Sepharose 4B column. Strongly enriched mTNF-α mutein was found in the flow through and wash as well as in the 100 % B eluate (20 mM ethanol amine, 10% ethylene glycol, pH 9.0). All TNF-α containing fractions were collected and both pools were dialysed separately against 20 mM Tris-HCl, pH 7.2 and loaded on a Q Sepharose (Pharmacia) , equilibrated with the same buffer. The chromatographical conditions were identical to thoεe deεcribed aε for wt mTNF-α (see section 7.1). SDS PAGE under reducing conditions showed that from the Phenyl Sepharose 4B eluate a preparation of more then 95% pure T105A TNF material could be obtained with a specific activity of ±1 x 108 U/mg as asεeεεed by bioaεεay on L929 cellε in the preεence of Actinomycin D. 7.4 Purification of tip deleted mTNF-α mutein (del mTNF-α) Cell lysis, PEI treatment of the supernatant aε well aε the (NH4)2S04 fractionation were identical aε these described for the P106A mTNF-α mutein. The chromatography on Phenyl Sepharose 4B was identical to thiε deεcribed in 7.1 (wt mTNF) except that 7,5 cell culture equivalent waε loaded on a 40 ml column (this means 2 runs instead of 4) . The conductivity of the non-bound fraction of the phenyl εepharoεe runε waε adjusted to 10 mS above this of the equilibration buffer of the Phenyl Sepharose 4B column and the material waε loaded on a 10 ml TSK butyl 650 M column (Merck) (flow rate: 1,5 cm/min,* 10 mg protein/ml gel; protein quantification baεed on abεorbance at 280 mm) . The bufferε for chromatography on TSK butyl 650 M are identical to thoεe uεed in the chromatography on Phenyl Sepharose 4B. After an extensive wash with equilibration buffer (12 column volumes) , bound proteins were eluted by applying a linear gradient over 16 column volumeε (100% A - 100% B) . The non-bound protein fraction was dialysed against 25 mM Triε-HCl, pH 9.0 and applied at lcm/min on a Q Sepharoεe FF column (Pharmacia) (> 5mg protein/ml gel) . Bound proteinε were eluted at 1 cm/min by applying a linear salt gradient (0 - 300 mM NaCl) , followed by a 300 mM NaCl wash (2 column volumeε) . SDS- PAGE shows that the del mTNF-α protein was found all over the eluate, but only the fractions eluting between 70-230 mM NaCl were pooled. These fractions were lacking the contaminating E. coli proteinε with a molecular weight of about 14000, 15000 and 70000. The pooled fractionε were diluted with the 25 mM Triε-HCl, pH 9.0 buffer and incubated for about 4 h at 4°C. Thereafter, the del mTNF-α pool waε reloaded on the Q-sepharose FF column but now at a concentration < 300 μg protein/ml gel (protein quantity based on abεorbance at 280 nm) . The waεh and elution conditionε were identical to these described for the first chromatography on Q Sepharoεe. The tip-deleted mTNF-α protein eluted in 2 well separated peaks with pure del mTNF-α protein in the second peak (elution at about 280 mM NaCl) . The material haε a specific activity of about 1 x 108 U/mg of protein as asεessed by bioasεay on L-929 cellε in the preεence of Actinomycin D. 7.5. Purification of the T105A-E107A mTNF-α double mutant To the bacterial cell εuεpenεion, aminocapronic acid, PMSF and DDT weree added to a final concentration of respectively 25 mM, 1 mM and 1 mM before lyεing by French Preεε (14,000 psi, AMINCO, SLM Instrumentε, Urbana, USA) . Subεequently, the suspension was centrifugated for 20 minutes at 16,500 rpm at 4°C uεing a JA-20 rotor (Beckman) . The mTNF-α double mutant could be found back in the supernatant fraction as aεεessed by SDS-PAGE and therefore only this fraction was uεed for further purification. The nucleic acidε in the εupernatant were precipitated by the addition of polyethylene-immine to a final concentration of 0.4% followed by centrifugation for 20 minuteε at 16,500 rpm at 4°C. The mTNF-α double mutant precipitated only partially after polyethylene imine treatment and waε recovered from the pellet by introducing a washing step with 0.5 ml NaCl pH 7.2. Both fractions were then enriched by a differential amoniumsulphate precipitation whereby a 40% εtep iε followed by a second step at 65% saturation. The mTNF-α mutein remained in the 65% amonium sulphate pellet. The pellet was resuspended in 25 mM Tris-HCl pH 7.2 and the conductivity of the solution waε adjusted to 10 mS above the conductivity of the Phenyl Sepharose 4B column startbuffer (25 mM TriεHCl pH 6.9, 25% (NH4)2S04) . The material waε loaded at 0.5 cm/minute on the Phenyl Sepharoεe 4B matrix (Pharmacia) and waεhed with 8 column volumeε of εtarting buffer. Proteinε bound to the matrix were eluted with a linear εalt gradient of 0% to 60% buffer B (20 mM ethanolamine, pH 9.0) over 6 column volumes. The flow through fractions containing the mTNF-α double mutant protein were pooled and dialysed overnight against 25 mM Tris-HCl pH 7.2. After centrifugation, the material waε loaded at l cm/minute on Q Sepharoεe FF (Pharmacia) equilibrated againεt 25 mM Triε-HCl pH 7.2. The column was washed with 4 column volumes of εtarting buffer and eluted with a linear εalt gradient from 0 mM to 300 mM NaCl in the εame buffer followed by εtepwise elution with 2 column volumes of 300 mM NaCl and 2 column volumeε of 1 mM NaCl. The mTNF-α double mutant eluted at 300 mM NaCl. A further purification waε performed on a column of Fractogel TSK butyl 650M (Merck) . To the protein solution, 25% (NH4)2S04 was added and loaded on the column at a flow-rate of 1 cm/minute (3 mg/ml gel) . The mTNF-α mutein protein waε recovered in the flow through fraction and the proteinε bound to the matrix were eluted with 10 column volumeε of a linear gradient from 0% to 100%. Aε a final poliεhing εtep the T105A- E107A mTNF protein waε purified by gel filtration on a Superdex 75 HR 10/30 column (Pharmacia) whereon it eluted aε the εecond (or largeεt) protein peak with an apparent weight of 66 kD. 7.6 Purification of the T105A-E107A-E110A mTNF-α triple mutant To the bacterial cell εuεpenεion, aminocapronic acid, PMSF and DDT waε added to a final concentration of reεpectively 25 mM, 1 mM and 1 mM before lysing by French Press (14,000 psi, AMINCO, SLM Instruments, Urbana, USA) . Subsequently, the εuεpenεion waε centrifugated for 20 minutes at 16,500 rpm at 4°C using a JA-20 rotor (Beckman) . The mTNF-α mutein could be found back in the εupernatant fraction as asεeεεed by SDS-PAGE. The nucleic acidε in the supernatant was then precipitated by the addition of polyethylene-imine to a final concentration of 0.4% followed by centrifugation for 20 minutes at 16,500 rpm at 4°C. The mTNF-α triple mutant was then enriched by a differential amoniumsulphate precipitation whereby a 40% εtep iε followed by a εecond εtep at 65% εaturation. mTNF-α or muteinε remain in the 65% amonium εulphate pellet. The pellet was resuspended in 25 mM Tris-HCl pH 7.2 and the conductivity of the solution was adjuεted to 10 mS above the conductivity of the Phenyl Sepharoεe 4B column startbuffer (25 mM Tris-HCl pH 6.9, 25% (NH4)2S04). The material waε loaded at 0.5 cm/minute on the Phenyl Sepharoεe 4B matrix (Pharmacia) and washed with 8 column volumes of εtarting buffer. Proteinε bound to the matrix were eluted with a linear εalt gradient of 0% to 60% buffer B (20 mM ethanolamine, pH 9.0) over 6 column volumeε. The fractionε containing the mTNF-α triple mutant (thiε iε the flow through and the 20 to 40% eluate fractionε) were pooled and loaded on a TSK-C4 650M (Merck) at 1 cm/h (10 mg/ml) and waεhed with 8 column volumeε of Phenyl Sepharoεe εtarting buffer. The proteins bound to the matrix were eluted with a linear gradient of 10 column volumes from 0% B to 100% B (20 mM ethanolamine pH 9.0) . The flow through fractions containing the mTNF mutein protein were pooled and loaded at a flow rate of 1 cm/min (5 mg/ml protein) on a Cu2+ IDA Sepharose fast flow column (Pharmacia) equilibrated against 20 mM sodium phosphate, 500 mM NaCl pH 6.8. The protein bounding to the matrix were eluted with a linear gradient from 0% to 70% B (20 mM sodium phosphate, 0,75 M NH4C1 pH 7.8) during 10 column volumes, one column volume of 70% B and one column volume of 100% B. The protein of interest eluted in the 70% B fraction. The fractions were pooled and dialysed against 25 mM Tris-HCl pH 9,0. The material was then loaded on a Q Sepharose FF (Pharmacia) column equilibrated against 25 mM Tris-HCl pH 7.2 at 1 cm/minute. The column was washed with 4 column volumes of starting buffer and eluted with a linear salt gradient from 0 mM to 300 mM NaCl in the same buffer followed by stepwise elution with 2 column volumes of 300 mM NaCl. The triple mTNF-α mutant eluted at 250 mM NaCl. EXAMPLE 2; The trypanocidal region of TNF-α contains a lectin-like activity of the molecule 1. Recombinant mTNF-α and hTNF-α are trypanocidal for T. brucei brucei and T. brucei rhodesiense and the effect is potentiated by cotreatement with LiCl Incubation of purified bloodstream forms of T. brucei brucei (Fig. 3 A) and T. brucei rhodesiense (Fig. 3 B) for 5 h with rm/hTNF-α reεultε in the mortality of a part of the paraεiteε: 50% of the animalε are killed with 5000 pg/ml of rm/hTNF-α on T. brucei brucei and T. brucei rhodesiense . In the εame assay, equal concentrations of hTNF-β, mouεe interleukin lα or mouεe interleukin lβ do not cauεe any mortality of T. brucei brucei or T. brucei rhodesiense (data not εhown) . Aε shown in Fig. 4, this trypanocidal effect of TNF-α is potentiated 100-fold by co- treating the trypanosomes with 1 μg/ml of LiCl, demonstrating that not only the cytotoxic effect of TNF-α on tumor cells (Beyaert et al. , 1989) but also the trypanocidal activity of the molecule is increased by LiCl. 2. The trypanocidal activity of mTNF-α in vi tro is inhibited by preincubating TNF-α with a trypanosome lysate, whereas preincubation of the mTNF-α with the soluble p55 hTNF-α receptor protein does not block thiε effect Preincubation of the mTNF-α during lh with different concentrationε of a lyεate from T. brucei brucei prepared aε described in the materials section potently inhibitε the mTNF-α trypanocidal effect (Fig. 5a) , whereaε the tumoricidal activity of mTNF-α on L929 cellε iε not affected (Fig. 5b) . On the other hand, preincubation of the mTNF-α during 1 h with 5 μg/ml of the εoluble p55 hTNF-α receptor potently inhibitε the mTNF-α tumoricidal activity against L929 cells, whereas the trypanocidal effect iε hardly influenced (Table III) . Thiε reεult clearly demonεtrates that the region responεible for the trypanocidal activity of the TNF-α molecule can be separated from the cellular p55 receptor binding domain on the TNF-α protein. TABLE III: Inhibitory capacity of εoluble p55 hTNF receptor on the mTNF-α tumoricidal and trypanocidal activity mTNF-α Trypanocidal activity Tumoricidal activity (pg/ml) (% lysis) (% lysiε) -p55 +p55 Δ -p55 +p55 Δ 100 30±2 25±3 15±5 40±2 O±l 100±5 1000 45±5 41±4 10±4 92±3 O±l 100±4 10000 60±4 56±3 7±5 98±4 93±3 5±2 Table III: Δ :% inhibition of activity by the addition of the p55 hTNF-α receptor protein 3. The TNF-α lectin-like region is probably involved in the trypanocidal activity of the protein Aε εhown in Fig. 6A, preincubation of mTNF-α for 1 h with 1 μg/ml of N,N' -diacetylchitobioεe, an oligoεaccharide for which TNF-α haε been εhown to have a lectin-like affinity (Hession et al. , 1987; Sherblom et al. , 1988), results in a potent inhibition of the mTNF-α trypanocidal effect, whereas the mTNF-α tumoricidal activity aε meaεured on L929 cells iε not affected (Fig. 6B.) Moreover, preincubation of the T. brucei brucei trypanoεomeε with lectinε (added at a concentration of 10 μg/ml) that have a specificity for N,N' -diacetylchitobiose, such as Urtica dioica agglutinin and Tri ticum vulgar is agglutinin, also results in an inhibition of the mTNF-α trypanocidal activity, whereas lectins with a different specificity (and added at the same concentration of 10 μg/ml) , εuch aε Galanthus nival is agglutinin and Griffonia simpl i ci fol i a A4, did not have any effect upon preincubation with the mTNF-α (Table IV) . Moreover, none of theεe lectins had any effect on the tumoricidal activity of TNF-α on L929 cells. Theεe results strongly suggest that the TNF-α lectin-like region is involved in the trypanocidal, but not in the tumoricidal activity. TABLE IV: Inhibitory capacity of different lectins on mTNF-α mediated trypanocidal activity againεt T. brucei brucei mTNF-α % inhibition of trypanocidal activity (pg/ml) WGA UDA GNA GSI-A4 100 70 ± 4 80 ± 2 O ± l 0 ± 2 1.000 63 ± 5 63 ± 3 5 ± 3 0 ± 2 10.000 13 ± 5 29± 5 5 ± 2 2 ± 1 100.000 5 ± 3 3 ± 2 3 ± 2 0 ± 2 1.000.000 7 ± 5 2 ± 1 2 ± 1 O ± l WGA Triticum vulα → aris agglutinin UDA : Urtica dioica agglutinin GNA : Galanthus nivalis agglutinin GSI-A4 : Griffonia simpl ici folia A4 All lectins were added at the concentration of 10 μg/ml Example 3; The tip region of mouse and human TNF-α contains the trypanocidal activity and lectin-like activities of the molecule and is involved in the TNF-induced lethal shock 1. Design of the TNF-α-derived peptides The biotinylated tip peptideε, inspired on the sequence spanning amino acid poεitions 100 to 116 of hTNF-α (Pennica et al. , 1984) and mTNF-α (Fransen et al., 1985) were εyntheεized aε indicated in the materialε and methods section. Subεequently, the peptideε were analyzed uεing a C18 reverεed phaεe HPLC column. Analyεis of the three-dimensional structure of the TNF-α molecule upon consulting the Brookhaven data base demonstrated that the identified epitope is located at the tip of the molecule. Thiε location iε intereεting conεidering the fact that TNF-α was shown to be active in a membrane-bound form (Kriegler et al., 1988). The following substitutions in both m/hTNF-α tip regions were made: P(100) by C, C(101) by G, and E(116) by C. It εhould be noted that the wild-type εequence of TNF-α containε a Cyε reεidue at location 101. In order to prevent inopportune closing of the loop with this Cyε(101), resulting in an unwanted conformation, the Cys 101 residue was mutated to a Gly. It has been demonstrated that this mutation did not affect the cytotoxic activity of native TNF-α, indicating that thiε amino acid is not involved in the TNF-receptor binding (Arakawa et al. , 1990) . Furthermore, native TNF-α as well as the tip peptide exert a trypanocidal activity as aεεayed by trypanoεome εurvival in the in vi tro aεεay. An overview of all the peptides syntheεized iε given in Table V below. TABLE V: Overview of εynthetic TNF-α tip region peptideε mTNFα-tip Bio-GG-CGPKDTPEGAELKPWYC (SEQ ID NO 24) hTNFα-tip Bio-GG-CGQRETPEGAEAKPWYC (SEQ ID NO 25) εubpep 1 Bio-GG-C TPEGAE C (SEQ ID NO 26) εubpep 2 Bio-GG EGAELKPWY- (SEQ ID NO 27) εubpep 3 Bio-GG TPE (SEQ ID NO 28) εubpep 4 Bio-GG-c tpeGae c (SEQ ID NO 29) mutpep 1 Bio-GG-C APEGAE C (SEQ ID NO 30) mutpep 2 Bio-GG-C TAEGAE C (SEQ ID NO 31) mutpep 3 Bio-GG-C TPAGAE C (SEQ ID NO 32) mutpep 4 Bio-GG-C TPEAAE C (SEQ ID NO 33) mutpep 5 Bio-GG-C TPEGAA C (SEQ ID NO 34) The sequences are given in the one letter amino acid code. The amino acids in the L or D configuration are anotated in the one letter code in capitals or in εmall letterε, reεpectively. The annotation Bio-GG- denoteε Biotin-Gly-Gly. 2. The synthetic mTNF-α tip peptide is directly trypanocidal in vi tro , an effect that can be inhibited by preincubation of the peptide with N,N' -diacetylchitobioεe To prove that the tip region of mTNF-α iε directly implicated in itε trypanocidal and itε lectin-like effect, experiments were designed to test (i) whether the synthetic mTNF-α-tip peptide has any trypanocidal activity in vi tro and (ii) if so, whether this activity can be inhibited by preincubating the peptide with N,N' -diacetylchitobioεe. Aε εhown in Fig. 7, the mTNF-α-tip peptide indeed εhows a trypanocidal activity against bloodstream forms of T. brucei brucei . whereas it exerts no tumoricidal activity on L929 cells when tested in a similar doεe reεponεe curve (data not εhown) . The hTNF-α-tip peptide shows similar resultε (Fig. 7) . In the in vi tro trypanocidal aεsay, h/mTNF-α haε a εpecific activity of 2 x IO 5 U/mg of protein, while the peptide exertε itε activity with an average specificity of 4 x IO 2 - 4 x IO 3 U/mg of peptide. Interestingly, the trypanocidal activity of the peptide can also be inhibited by preincubating it for 1 h with 1 μg/ml of N,N' -diacetylchitobiose (Fig. 7), demonstrating that the tip region of h/mTNF-α (from amino acid position 100 to 116) is involved in its trypanocidal and its lectin-like activity. 3. The short tip peptide, Thr Pro Glu Gly Ala Glu (SEQ ID NO 23) , contains the trypanocidal activity of TNF-α and iε linked with the deεcribed lectin-like activity of the protein since it can be inhibited by preincubation with N,N'-diacetylchitobiose The fact that both human and mouse TNF-α peptides exert in vi tro trypanocidal activity with no significant differences in specific activity also implieε that the amino acids which are common to the human and the mouse TNF-α tip region can be involved in the trypanocidal activity. Thiε hypotheεiε waε further inveεtigated by teεting the trypanocidal activity of two subpeptideε of the tip region containing εequenceε εimilar to both εpecieε. The εubpeptide 1, which consistε of the positionε T(105), P(106), E(107), G(108), A(109) and E(110) which are identical in m/hTNF-α εtill had full trypanocidal activity (Fig. 8), whereaε εubpeptide 2 which encompaεses the poεitionε E(107) to Y(ll5) of mTNF-α (with only L(lll) different from hTNF-α) showed strongly reduced trypanocidal activity (Fig. 9) . These resultε εtrongly suggest that the trypanocidal and, thus, perhaps also the lectin-like activity of TNF-α, iε at leaεt partially mediated by amino acid poεition T(105) to E(110), in which eεpecially poεitionε T(105) and/or P(106) εeem(s) to be of the utmoεt importance. The trypanocidal activity of the different mutant peptideε 1, 2, 3, 4 and 5 of h/mTNF-α waε meaεured, wherein positions 105, 106, 107, 108 or 110 of the TPEGAE peptide were replaced consecutively by an A. The mutant peptides in which the T(105) or E(107) or E(110) waε replaced by an A loεt their trypanocidal activity, while the two other mutantε were fully or even more active (Fig. 8 and 9) . Compariεon of the trypanocidal activity of the fully active TPEGAE peptide with the synthetic oligopeptide tpeGae wherein the L amino acidε were replaced by D reεidues demonstrated the stereoεpecificity of the TNF-α tip peptide trypanoεome interaction since the latter peptide had lost its activity (Fig. 8) . EXAMPLE 4 : Antibodies raised against the tip region of mouse and human TNF-α inhibit the trypanocidal acitivity and the septic shock effects but not the tumoricidal activity of TNF-α. 1. Generation of rabbit anti-tip mTNF-α and hTNF-α polyclonal antibodies Polyclonal antibodies against the mTNF-α and hTNF-α-derived tip peptides were produced as follows: On day 0, rabbits were injected subcutaneouεly with 50 μg of a complex existing of 7 μg of the biotinylated tip peptide and 43 μg of avidin (Neutralite, Eurogentec, Belgium) dissolved in complete Freund's adjuvant (total volume: 1 ml) (Difco Laboratorieε, Detroit, MI, USA) . On day 21, rabbitε were injected εubcutaneously with 50 μg of the same complex disεolved in incomplete Freund'ε adjuvant (total volume: 1 ml) . On day 42, the rabbitε were injected subcutaneously with 100 μg of the avidin-tip peptide complex in 1 ml of PBS. On day 52, the rabbits were bled, and the sera which reacted positively in ELISA with both the synthetic h/mTNF-α tip peptide bound to εtreptavidin and with the native h/mTNF-α molecule, were purified on Protein G Sepharoεe CL4B (Sigma, St. Louiε, MO, USA) essentially as proposed by the supplier. The IgG concentration was asseεεed by the Bradford aεεay. 2. Generation of anti-tip mTNF-α monoclonal antibodieε 2.1. Immunization Balb/c mice were immunized with 100 μg of avidine-biotine- TNF-α tip peptide conjugateε emulεified in complete Freund'ε adjuvant. Three weekε later the εame animals were challenged with 400 μg of avidin. Biotin-TNF-α tip peptide conjugates emulsified in incomplete Freund's adjuvant. Another three weeks later, the mice were boosted with 10 μg of avidin-biotin-TNF-α tip peptide conjugateε diεεolved in PBS. Three dayε later the εpleen cellε were fuεed to generate monoclonal antibodieε. 2.2. Fuεion and screening Approximately IO 8 spleen cellε of immunized Balb/c mice were fuεed with the univerεal B cell fuεion partner NSO via the PEG- fuεion protocol (Galfre and Milεtein, 1981) . Poεitive hybridomaε were identified via an ELISA εcreening on ELISA plateε coated with TNF-α. The thuε obtained anti-TNF-α tip monoclonal antibodieε did not neutralize the tumoricidal activity of TNF-α. 3. Differential inhibition of the trypanocidal and the tumoricidal activities of mTNF-α by monoεpecific polyclonal anti-mTNF-α tip peptide antibodies or monoclonal mTNF-α anti-tip antibodies. Preincubation of mTNF-α for 1 h at room temperature with 10 μg/ml of protein G-sepharose-purified rabbit-anti-rmTNF-α tip peptide polyclonal antibodieε or with a mouεe anti-rmTNF-α tip peptide monoclonal antibody results in a strong inhibition of the mTNF-α-mediated trypanocidal effect (Fig. 10) , whereas only a weak inhibition of the mTNF-α-mediated tumoricidal effect on L929 cells waε detected (data not εhown) . The reεultε εtreεε once more that this region of the TNF-α molecule is directly involved in the trypanocidal and in the lectin-like activity of TNF-α. 4. Anti-mTNF-α tip polyclonal antibodies can inhibit LPS-induced septic shock in vivo Sherblom et al. (1988) have shown that TNF-α has a lectin-like affinity for the glycoprotein uromodulin. Interestingly, thiε glycoprotein iε able to potently inhibit the LPS-induced εhock in vivo, whereaε it doeε not block the tumoricidal activity of TNF-α on L929 cellε in vi tro . The authorε εuggeεted therefore that the lectin-like region of TNF-α iε implicated in the toxic effectε of TNF-α in εeptic εhock, but not in itε tumoricidal activity on L929 cellε. Since the TNF-α tip region is involved in the lectin-like activity of TNF-α, as demonstrated in the previouε εectionε, the effect of the rabbit anti-mTNF-α tip polyclonal antibodieε on LPS-induced εeptic εhock in vivo waε investigated. As shown in Table V, intraperitoneal injection of Balb/C mice (10 mice/group) with 100 μg of protein G-purified anti-tip polyclonal antibodies, 2 h before the injection of the lethal doεe of 500 μg of LPS/mouse, resulted in an increaεed εurvival time aε compared with the control mice that only received PBS or an irrelevant control polyclonal antibody preinjection (control IgG) . Thiε result indicates that (pre)treatement of the animal with anti-m/hTNF-α tip antibodies can protect the animal from the lethal effects of the septic shock syndrome. TABLE VI : Effect of lethal dose of LPS on εurvival time of mice EXAMPLE 5 ; Tip-mutated or tip-deleted TNF-α is not hampered in its in vi tro cvtolytic/cytotoxic activity and has a reduced lethal effect 1. Recombinant and mutated mTNF-α and hTNF-α are cytotoxic to L-929 cells in vitro. The purified recombinant mTNF-α and hTNF-α, produced in E.coli, had a specific activity of respectively, 2-3108 Units/mg and 2-4 107 Units/mg aε teεted on εpecific cell lysis of L-929 cells in the 18 hours assay in the presence of Actinomycin D. The mTNF-α tip-mutations or -deletion did not hamper the in vitro cytolytic activity of the molecule (Fig 11) . 2. T(105), E(107), and/or E(110) mutated or tip deleted TNF- α has.loεt the mTNF-α trypanocidal activity. The T(105) , E(107), and/or E(110) mutantε, combinationε of theεe and deletionε of the TPEGAE human or mouse TNF-α tip domain result in a significant loεε of mTNF-α trypanocidal activity (Fig. 12) . It iε to be remarked in thiε context that, mutation of the P(106) and/or G(108) in the tip peptide to A did not change the trypanocidal nor the cytolytic activity of TNF-α (data not εhown) . 3. T(105), E(107) and/or E(110) tip-mutated or tip-deleted mTNF-α haε a lower LD50 value then wild type mTNF-α. Wild type mTNF-α iε lethal for C57BL/6 mice at a dose of 10 μg/mouse. It was tested if those mutations hampering the trypanocidal activity of mTNF-α (which could in previous examples be correlated with the lectin-like activity of the molecule) had any influence on the LD50 of the protein. Aε these TNF-α muteins of the present invention exert the εame cell cytotoxic activity aε wild type TNF-α and aε thiε effect iε directly linked with the in vivo tumoricidal activity of the cytokine, the TNF-α muteinε of the invention could be uεeful aε anti-tumor agens without the nefast toxic εide effectε of TNF-α. Moreover, any activity of TNF-α that could be linked with itε lectin-like activity, εuch aε induction of the septic shock εyndrome, cachexia, the damage to endothelial cells, etc., could then be circumvented by using the above deεcribed TNF-α muteinε. Figure 13 εhowε the εurvival rate of intravenouεly injected C57BL/6 mice when treated with 10 μg, 5 μg, 2.5 μg or 1 μg/mouεe of either wild type mTNF-α or T105A mTNF-α (6 mice/group) . At the the doεe of 5 μg/mouεe a differential effect of the wild type and the T105A mTNF-α could be observed. EXAMPLE 6 : The tip region of the TNF-α molecule has an effect on the in vivo half life of the molecule Since lectin-carbohydrate interactions ~ may modulate the clearance of a protein, the effect of mutations in the tip region on the in vivo half life of the molecule was tested. Female 8 weeks old C57bL/6 mice (6 mice/group - Charleε Rivers Deutschland) were injected intraperitoneally with 1 μg/mouεe of either wild type mouεe TNF-α or tip deleted TNF-α. Subεequently, the mice were bled 0.5, 5, 10, 15, 30, 60, 120, 180, or 240 minuteε post-injection. The sera were tested for mTNF-α contentε in the L-929 cytotoxic assay and in the indirect ELISA asεay aε detailed in the materials and methods sections detecting both wild type and tip mutated mouse TNF-α with the same sensitivity. The results of 2 experiments are given in Table VII below. Theεe experiments demonstrate a εignificant longer half life (at least 2 fold longer half life) for the tip deleted TNF-α mutant than for the wild type, TNF-α molecule. Table VII: In vivo half life of mouse wild type and tip-deleted TNF-α. Experiment 1 time post concentration TNF-α (ng/ml) injection (minuteε) wild type TNF tip deleted TNF 0.5 1000 1000 5 880 990 10 650 640 15 500 550 30 420 580 60 320 450 120 20 250 180 10 100 240 0 10 Experiment 2 time post concentration TNF-α (pg/ml) injection (minutes) wild type TNF tip deleted TNF 0.5 1000 1000 15 550 600 30 500 500 60 480 500 120 20 330 180 0 240 240 0 20 EXAMPLE 7 ; The tip region of the TNF-α molecule is involved in the induction of endothelial cells to produce inflammatory cytokines 1. Tip-mutated TNF-α induceε leεε human interleukin-6 (IL6) on human microvaεcular enothelial cellε of the brain than wild type TNF-α Human microvaεcular endothelial cellε of the brain (hMVECb) are prepared as described by Grau and Lou (1993) . The cellε were plated in 24 well plateε at 106 cells/ml and treated for 24 hours with variouε doεeε of wild type or tip- mutated TNF-α. Subεequently, the IL6 concentration in the supernatant of the cells waε aεεeεεed uεing a commercially available hIL6 ELISA kit (Medgenix, Belgium) . The reεultε (εhown in Table VIII) demonεtrate that tip mutated TNF iε leεε capable of inducing the endothelial brain cells to produce IL6. Table VIII : IL6 production (pg/ml) by hMVECb cells by treatment with wild type mouse TNF-α or tip mutated mTNF-α pg/ml IL6 concentration (pg/ml) experiment 1 experiment 2 wt mTNF: 0 620 800 333 1520 1900 1000 1250 1400 1666 1220 1300 del mTNF: 0 620 800 333 760 850 1000 1180 1600 1666 1240 1900 T105A mTNF: 0 620 800 333 560 700 1000 1080 1300 1666 980 1250 P106A mTNF: 0 620 800 333 720 1300 1000 560 750 1666 800 1300 2. The T105A and the P106A mTNF-α mutants induce less human interleukin 8 (IL8) than wild type TNF-α on human endothelial brain cells The conditionned medium of 106 hMVECb cells/ml treated for 24 hourε with different doses of wild type or tip mutated TNF-α was analysed on IL8 production using a commercially available human IL8 ELISA detection kit (Medgenix, Belgium) . The resultε, εhown in Table IX below, demonεtrate that the T105A and P106A mutantε are inducing about 3 fold leεs IL8 in these cellε than wild type TNF-α while the tip deletion mutant was about two times more powerfull in inducing the inflammatory cytokine IL8. Table IX : IL8 production (pg/ml) by hMVEC6 cells by treatment with wild type mouse TNF-α or tip-mutated mTNF-α pg/ml IL8 concentration (pg/ml) experiment 1 experiment 2 wt mTNF: 0 40 80 333 1050 1700 1000 1680 1800 1666 1200 1650 del mTNF: 0 40 80 333 1800 2450 1000 1300 2200 1666 850 1250 T105A mTNF: 0 40 80 333 600 750 1000 800 1250 1666 380 500 P106A mTNF: 0 40 80 333 300 400 1000 1100 1500 1666 250 900 EXAMPLE 8 : The tip region of TNF-α is involved in the metastatis-promoting effect of the molecule Beεideε the well defined anti-tumor effect of TNF-α, experimentε concerning the influence of exogenous TNF-α on metastaεiε has been a point of discuεεion. In a murine fibroεarcoma model (Tomazic et al. , 1988), a lung cancer model (Schlutz and Altom, 1990) and in melanoma modelε (Sylveεter et al, 1990; Lollini et al. , 1990) inhibition of formation of metaεtaεeε by recombinant human TNF-α haε been reported when the latter waε given within 3 dayε of tumor cell inoculation. On the other hand, adminiεtration of recombinant human TNF-α led to an increaεe of pulmonary metaεtaεiε when given 1 hour prior to the intravenous inoculation of a human melanoma cell line into nude mice (Giavazzi et al. , 1990) or promoted the implantation of human ovarian cancer xenograftε in the peritoneal cavity of nude mice (Malik et al. , 1989). The metastaεiε-promoting effect of wild type and tip deleted mouεe TNF-α waε compared by uεe of the 3LL-R tumorigenic Lewiε lung carcinoma cell line (wt and del mTNF-resistant Lewis lung carcinoma) (Remels et al. , 1989). Thereto, 8 weeks old female C57bl/6 mice (5 mice/group) (Charles River, Deutεchland) were injected intraperitoneally with either PBS (control group) or 5 μg of wild type or tip-deleted mouse TNF-α per mouse, 5 hours prior to the intravenous injection of 2.106 3LL-R cells. This tumor inoculum normally leads to a 100% mortality 25 dayε after injection of the control group. After 17 days, the mice injected with wt mTNF/3LL-R all suffered from severe reεpiration problemε whereaε the mice in the del mTNF/3LL-R group looked εignificantly better and the mice in the PBS/3LL-R group looked completly healty. At thiε time, all mice were killed and the lung weight aε well aε the amount of cancer cell noduleε in the lung waε aεεeεεed. The results are shown in Table X below. The weight of the lungs as well as the number of nodules present in the lungs was significantly higher (at least lOOx) in the TNF-α treated mice indicating that TNF-α indeed iε capable of enhancing the metaεtatic potential of TNF-reεiεtant tumor cellε. However, the metaεtatic enhancing potential of the TNF tip deletion mutant layε conεiderably lower than that of wild type TNF. The obεerved metaεtasis-promoting effect for TNF-α might reεide in an influence of TNF-α on the interaction of circulating tumor cellε with the endothelium εupporting the arreεt, diapedeεiε and extravasation of tumor cells. As the tip-deleted TNF-α protein has εignificantly leεε metaεtaεiε promoting capacity than wild type TNF-α, the tip region of the molecule may interfere with the interaction of TNF-α with the endothelium. Thiε interaction can be due to the enhanced expreεεion of adheεion moleculeε on endothelial cells and/or tumor cells by a direct action of TNF-α on theεe cellε or being mediated via additional cellε e.g. granulocyteε or plateletε. 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Vilchek J, Palombella V, Henrikεen-Deεtefano V, Swenεεon C, Fein an R, Hirai M, Tεujimoto M (1986) Fibroblaεt growth enhancing activity and its relationship to other polypeptide growth factorε J Exp Med 163: 632-643. Wang A, Creaεey A, Ladner M, Lin L, Strickler J, Van Arεdell J, Yamamoto R, Mark D (1985) Molecular cloning of the complementary DNA for human tumor necroεis factor. Science 228:149-154. SEQUENCE LISTING ID GENERAL INFORMATION: (i) APPLICANT: (A) NAME: Innogenetics N.V. (B) STREET: Industriepark Zwijnaarde 7 Bus 4 (C) CITY: Gent (E) COUNTRY: Belgium (F) POSTAL CODE (ZIP) : 9052 (G) TELEPHONE: 00-32-09.241.07.11 (H) TELEFAX: 00-32-09.241.07.9.9 (ii) TITLE OF INVENTION: TNF-MUTEINS, A PROCESS FOR PREPARING THEM AND THEIR USE AS ACTIVE SUBSTANCES IN PHARMACEUTICAL COMPOSITIONS (iii) NUMBER OF SEQUENCES: 34 (iv) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.25 (EPO) (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 14 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: YES (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: Xaa Xaa Xaa Thr Xaa Glu Xaa Xaa Gly Xaa Lys Pro Trp Tyr 1 5 10 (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 1 (D) OTHER INFORMATION: Xaa is Pro or Gin (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 2 (D) OTHER INFORMATION: Xaa is Lys or Arg (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 3 (D) OTHER INFORMATION: Xaa is Asp or Glu (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 5 (D) OTHER INFORMATION: Xaa is Pro, Ala, Thr, or Gly (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 7 (D) OTHER INFORMATION: Xaa is Gly, Ala, Thr, Pro, or Ser (ix) FEATURE (A) NAME/KEY: misc-feature ( B ) LOCATION : 8 (D) OTHER INFORMATION: Xaa is Ala, Pro, Gly, or Thr (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 10 (D') OTHER INFORMATION: Xaa is Leu or Ala (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 1..3 (D) OTHER INFORMATION: These amino acids may be present or absent (ix) FEATURE (A) NAME/KEY: misc-feature (B) LOCATION: 10..14 (D) OTHER INFORMATION: These amino acids may be present or absent (2) INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4009 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: circular (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vii) IMMEDIATE SOURCE: ( B ) CLONE : pIG2 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: TTCCGGGGAT CTCTCACCTA CCAAACAATG CCCCCCTGCA AAAAATAAAT TCATATAAAA 60 AACATACAGA TAACCATCTG CGGTGATAAA TTATCTCTGG CGGTGTTGAC ATAAATACCA 120 CTGGCGGTGA TACTGAGCAC ATCAGCAGGA CGCACTGACC ACCATGAAGG TGACGCTCTT 180 AAAAATTAAG CCCTGAAGAA GGGCAGGGGT ACCAGGAGGT TTAAATCATG GTAAGATCAA 240 GTAGTCAAAA TTCGAGTGAC AAGCCTGTAG CCCACGTCGT AGCAAACCAC CAAGTGGAGG 300 AGCAGCTGGA GTGGCTGAGC CAGCGCGCCA ACGCCCTCCT GGCCAACGGC ATGGATCTCA 360 AAGACAACCA ACTAGTGGTG CCAGCCGATG GGTTGTACCT TGTCTACTCC CAGGTTCTCT 420 TCAAGGGACA AGGCTGCCCC GACTACGTGC TCCTCACCCA CACCGTCAGC CGATTTGCTA 480 TCTCATACCA GGAGAAAGTC AACCTCCTCT CTGCCGTCAA GAGCCCCTGC CCCAAGGACA 540 CCCCTGAGGG GGCTGAGCTC AAACCCTGGT ATGAGCCCAT ATACCTGGGA GGAGTCTTCC 600 AGCTGGAGAA GGGGGACCAA CTCAGCGCTG AGGTCAATCT GCCCAAGTAC TTAGACTTTG 660 CGGAGTCCGG GCAGGTCTAC TTTGGAGTCA TTGCTCTGTG AAGGGAATGG GTGTTCATCC 720 ATTCTCTACC CAGCCCCCAC TCTGACCCCT TTACTCTGAC CCCTTTATTG TCTACTCCTC 780 AGAGCCCCCA GTCTGTGTCC TTCTAACTTA GAAAGGGGAT TATGGCTCAG AGTCCAACTC 840 TGTGCTCAGA GCTTTCAACA ACTACTCAGA AACACAAGAT GCTGGGACAG TGACCTGGAC 900 TGTGGGCCTC TCATGCACCA CCATCAAGGA CTCAAATGGG CTTTCCGAAT TCTCTAGAGT 960 CGACCTGCAG CCCAAGCTTG GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA 1020 GATTAAATCA GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC 1080 ■ GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG 1140 TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC 1200 AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA 1260 GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG 1320 CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG 1380 GCCTTTTTGC GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT 1440 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT AAAAGGATCT AGGTGAAGAT 1500 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC 1560 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 1620 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 1680 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT 1740 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 1800 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 1860 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 1920 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 1980 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 2040 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 2100 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 2160 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 2220 CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT 2280 TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC 2340 AGTGAGCGAG GAAGCGGAAG AGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA 2400 AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC 2460 ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA 2520 GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC 2580 CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG 2640 GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG 2700 TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC 2760 ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA 2820 CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT 2880 CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC 2940 TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG 3000 AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG 3060 CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG 3120 ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC 3180 GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG 3240 CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT 3300 GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG 3360 CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG 3420 CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC 3480 GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG 3540 ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT 3600 GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCCACAGG ACGGGTGTGG TCGCCATGAT 3660 CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG 3720 GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG 3780 CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG 3840 CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC 3900 GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA 3960 TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA 4009 (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: CATGCTCAGA TCAAGTAGTC AAAATT 26 (2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: CGAATTTTGA CTACTTGATC TGAGCATG 28 (2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: CAAGGACGCT CCGGAGGGGG CTGAGCT 27 (2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: CAGCCCCCTC CGGAGCGTC 19 (2) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: CAAGGACACC GCTGAGGGCG CCGAGCT 27 (2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single <D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: CGGCGCCCTC AGCGGTGTC 19 (2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) [iii) HYPOTHETICAL: NO [iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: CAAGGACACC CCTGCAGGCG CTGAGCT 27 (2) INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO .iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: CAGCGCCTGC AGGGGTGTC 19 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: CAAGGACACC CCTGAGGGCG CCGCCCT 27 (2) INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: AGGGCGGCGC CCTCAGGGGT GTC 23 (2) INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: CAAGGACACC CCTGCAGGCG CCGCCCT 27 (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: AGGGCGGCGC CTGCAGGGGT GTC 23 (2) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15: CAAGGACGCT CCGGAGGGCG CCGCCCT 27 (2) INFORMATION FOR SEQ ID NO: 16: (i) SEQUENCE CHARACTERISTICS: (A) 'LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) [iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16: AGGGCGGCGC CCTCCGGAGC GTC 23 (2) INFORMATION FOR SEQ ID NO: 17: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: CAAGGATGCC CCGGCGGGTG CAGAGCT 27 (2) INFORMATION FOR SEQ ID NO: 18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18: CTGCACCCGC CGGGGCATC 19 (2) INFORMATION FOR SEQ ID NO: 19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: CAAGGATGCT CCGGCCGGTG CGGCCCT 27 (2) INFORMATION FOR SEQ ID NO: 20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20: AGGGCCGCAC CGGCCGGAGC ATC 23 (2) INFORMATION FOR SEQ ID NO: 21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21: CAAGGACCTC AAACCATGGT ATGAGCCCAT ATAC 34 (2) INFORMATION FOR SEQ ID NO: 22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: YES (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: ATGGGCTCAT ACCATGGTTT GAGGTC 26 (2) INFORMATION FOR SEQ ID NO: 23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23: Thr Pro Glu Gly Ala Glu 1 5 (2) INFORMATION FOR SEQ ID NO: 24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24: Cys Gly Pro Lys Asp Thr Pro Glu Gly Ala Glu Leu Lys Pro Trp Tyr 1 5 10 15 Cys (2) INFORMATION FOR SEQ ID NO: 25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide [ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25: Cys Gly Gin Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr 1 5 10 15 Cys (2) INFORMATION FOR SEQ ID NO: 26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide [ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26: Cys Thr Pro Glu Gly Ala Glu Cys 1 5 (2) INFORMATION FOR SEQ ID NO: 27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 9 amino acids (B) TYPE*, amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27: Glu Gly Ala Glu Leu Lys Pro Trp Tyr 1 5 (2) INFORMATION FOR SEQ ID NO: 28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide [ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28: Thr Pro Glu 1 (2) INFORMATION FOR SEQ ID NO: 29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Modified-site (B) LOCATION: 1..8 (D) OTHER INFORMATION: /note= "D amino acids" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29: Cys Thr Pro Glu Gly Ala Glu Cys 1 5 (2) INFORMATION FOR SEQ ID NO: 30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30: Cys Ala Pro Glu Gly Ala Glu Cys 1 5 (2) INFORMATION FOR SEQ ID NO: 31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY : linear ( ii ) MOLECULE TYPE : pept ide [ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31: Cys Thr Ala Glu Gly Ala Glu Cys 1 5 (2) INFORMATION FOR SEQ ID NO: 32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32: Cys Thr Pro Ala Gly Ala Glu Cys 1 5 (2) INFORMATION FOR SEQ ID NO: 33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33: Cys Thr Pro Glu Ala Ala Glu Cys 1 5 (2) INFORMATION FOR SEQ ID NO: 34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 8 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (ix) FEATURE: (A) NAME/KEY: Binding-site (B) LOCATION: 1 (D) OTHER INFORMATION: /note= "Biotin-Gly-Gly is coupled to the N-terminus of the peptide" (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34: Cys Thr Pro Glu Gly Ala Ala Cys 1 5

Claims: CLAIMS 1. A tumor necrosis factor mutein characterized in that the TNF-α amino acid sequence is mutated, or deleted totally or partially, in the region extending from amino acid position at 101 to 116 in such a way that: - either the lectin-like activities are modulated with respect to TNF-α, - and/or the toxic activity is reduced with respect to TNF-α, - and/or the inflammatory cytokines inducing capacities are modulated with respect to TNF-α, - and/or the adhesion molecules inducing capacities are modulated with respect to TNF-α, - and/or the metastasis promoting activity is reduced with respect to TNF-α, - and/or the half life time is increased with respect to TNF-α, and providing that these TNF-α muteins have preferentially retained the tumoricidal activity of TNF-α, and providing that said TNF muteins are different from human TNF-α wherein amino acids 1 to 8 are replaced by a sequence within the region εpanning amino acids 5 to 30 of laminin, and providing that said TNF muteins are different from human TNF-α wherein: amino acid position 101 is Ser, hTNF-α wherein amino acid position 102 is Arg or deleted, hTNF-α wherein amino acid position 103 is Trp, hTNF-α wherein amino acid position 105 is Pro, hTNF-α wherein amino acid position 105 is lie, hTNF-α wherein amino acid position 105 is lie and position 44 is Cys, hTNF-α wherein amino acid position 106 is Ser, hTNF-α wherein amino acid position 106 is Ser and position 131 is Cys, hTNF-α wherein amino acid position 108 is Phe, hTNF-α wherein amino acid position 110 is Lys, hTNF-α wherein amino acid positions 111 to 112 are deleted, hTNF-α wherein amino acid position 112 is deleted or Met, hTNF-α wherein amino acid position 111 is deleted and amino acid positions 109 and 120 are respectively Gin and His, hTNF-α wherein amino acid position 115 is lie or Cys, hTNF-α wherein amino acid position 116 is Lys, His or Val, hTNF-α wherein amino acid positions 115-116 are Ile-Lys,* and with said TNF muteins possibly containing in their peptidic chain outside the region spanning amino acids 101 to 116 of TNF- α, additional modifications consisting of substitutions and/or deletions and/or additions of one or several amino acid residues, and with said muteins being characterized in that they have retained the aforementioned activities; or a pharmaceutically acceptable salt thereof. 2. A TNF mutein according to claim 1, further characterized in that the lectin-like activities are modulated with respect to TNF-α. 3. A TNF mutein according to any of claims 1 or 2, further characterized in that the lectin-like activities are increased with respect to TNF-α. 4. A TNF mutein according to any of claims 1 or 2, further characterized in that the lectin-like activities are reduced with respect to TNF-α. 5. A TNF mutein according to claim 1, further characterized in that the toxic activity is reduced with respect to TNF-α. 6. A TNF mutein according to claim 1, further characterized in that the inflammatory cytokine inducing capacities are modulated with respect to TNF-α. 7. A TNF mutein according to any of claims 1 or 6, further characterized in that the inflammatory cytokine inducing capacities are increased with respect to TNF-α. 8. A. TNF mutein according to any of claims 1 or 6, further characterized in that the inflammatory cytokine inducing capacities are reduced with respect to TNF-α. 9. A TNF mutein according to claim 1, further characterized in that the adhesion molecule inducing capacities are modulated with respect to TNF-α. 10. A TNF mutein according to any of claims 1 or 9, further characterized in that the adhesion molecule inducing capacities are reduced with respect to TNF-α. 11. A TNF mutein according to any of claims 1 or 9, further characterized in that the adhesion molecule inducing capacities are increased with respect to TNF-α. 12. A TNF mutein according to claim 1, further characterized in that the metastasis promoting activity is reduced with respect to TNF-α. 13. A TNF mutein according to any of claims 1 to 12, further characterized in that the tumoricidal activity is retained with respect to TNF-α. 14. A TNF mutein according to any of claims 1 to 12, further characterized in that the tumoricidal activity is reduced with respect to TNF-α. 15. A TNF mutein according to any of claims 1 to 14, further characterized in it shows an increased half life time with respect to TNF-α. 16. A TNF mutein according to any of claims 1 to 15, characterized in that at least part of the region extending from amino acid positions 101 to 116 of TNF-α, or the complete region corresponding to amino acid positions 101 to 116 of TNF-α has been deleted, and preferably at least the region covering amino acid positions 105 to 110 has been deleted. 17. A TNF mutein according to any of claims 1 to 15, characterized in that at least one of the amino acids in the region extending from amino acids 101 to 116 of TNF-α, and preferably at least one of the amino acids in the region extending from amino acids 105 to 110, has been mutated or deleted. 18. A Nucleic acid sequence encoding any of the polypeptides according to claims 1 to 17. 19. A process for the preparation of the polypeptides according to any of claims 1 to 17, comprising the steps of: - transformation of an appropriate cellular host with a vector, particularly a plasmid, a cosmid, a phage or a virus, in which a nucleic acid sequence according to claim 18 coding for at least one of the polypeptides according to any of claims 1 to 17 has been inserted (insert) under the control of the appropriate regulatory elements, particularly a promoter recognized by the polymerases of the cellular host and, in the case of a procaryotic host, an appropriate ribosome binding site (RBS) , enabling the expression in said cellular host of said nucleic acid sequence, - culture of said transformed cellular host under conditions enabling the expression of said insert. 20. A TNF mutein according to any of claims 1 to 17, for treating illnesses and pathological conditions such as, sepsis, septic shock, Gram negative sepsis, endo-toxic shock, toxic shock syndrome, cachexia, microbial infectionε, rheumatoid arthritis, inflammatory conditionε, reεpiratory diεtreεs syndrome, pulmonary fibroεis, infections, graft-versuε-hoεt-diεease, reperfusion damage such as myocardial ischaemia, AIDS, cancer, cerebral malaria, immunosuppresεion, etc. 21. A pharmaceutical compoεition, containing as active εubεtance, at leaεt anyone of the TNF mutein polypeptides according to any of claimε 1 to 17, in aεεociation with a pharmaceutical acceptable vehicle. 22. Uεe of a TNF mutein characterized in that the TNF-α amino acid εequence is mutated, or deleted totally or partially, in the region extending from amino acid position at 101 to 116 in εuch a way that: - either the lectin-like activities are modulated with respect to TNF-α, - and/or the toxic activity is reduced with respect to TNF-α, - and/or the inflammatory cytokines inducing capacities are modulated with respect to TNf-α, - and/or the adhesion molecules inducing capacitieε are modulated with reεpect to TNF-α, - and/or the metastasis promoting activity is reduced with respect to TNF-α, - and/or εhow an increased half life time with respect to TNF-α, and providing that these TNF-α muteinε have preferentially retained the tumoricidal activity of TNF-α, and with εaid TNF muteins posεibly containing in their peptidic chain outεide amino acid region 101 to 116 of TNF-α, additional modificationε conεiεting of εubεtitutionε and/or deletionε and/or additions of one or several amino acid reεidueε, and with εaid muteinε being characterized in that they have retained the aforementioned activities,* or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for treating illnesseε and pathological conditionε, εuch aε, εepεiε, εeptic εhock, Gram negative εepεiε, endo-toxic εhock, toxic εhock εyndrome, cachexia, microbial infectionε, rheumatoid arthritiε, inflammatory conditionε, reεpiratory diεtreεs syndrome, pulmonary fibrosiε, infectionε, graft-versus-hoεt-disease, reperfusion damage such aε myocardial iεchaemia, AIDS, cancer, cerebral malaria, immunoεuppression, etc. 23. Use of an antibody specifically detecting an epitope residing in the region comprising amino acids 101 to 116 of TNF-α, more particularly a monoclonal antibody, characterized in that it: - either modulates the lectin-like activitieε of TNF-α, - and/or inhibitε the toxic activity of TNF-α, - and/or modulateε the inflammatory cytokineε inducing capacitieε of TNf-α, - and/or modulateε the adhesion molecules inducing capacities of TNF-α, - and/or inhibitε the metaεtaεiε promoting activity of TNF-α, for the preparation of a medicament for treating TNF-induced septic shock or illnesεeε or pathological conditionε aεεociated with the in vivo activities of TNF-α. 24. Use of an immunological complex comprising a monoclonal antibody according to claim 23, and complete TNF-α, for the preparation of a medicament for treating illnesses such as tumors. 25. Use of an antiεense peptide of a peptide comprising at least part of the region 101 to 116 of TNF-α, more particularly a monoclonal antibody, characterized in that it : - either modulates the lectin-like activitieε of TNF-α, - and/or inhibitε the toxic activity of TNF-α, - and/or modulateε the inflammatory cytokineε inducing capacitieε of TNf-α, - and/or modulateε the adheεion moleculeε inducing capacitieε of TNF-α, - and/or inhibitε the metaεtaεis promoting activity of TNF-α, fo the preparation of a medicament for treating TNF-induced septic shock or illnesseε or pathological conditions aεsociated with the lectin-like effectε of TNF-α. 26. Uεe of a complex compriεing an antisense peptideε according to claim 25 and TNF-α for the preparation of a medicament for treating illnesses such aε tumorε. 27. Uεe of an antibody or antiεenεe peptide according to any of claims 23 to 26, characterized in that said antibody or said peptide modulates the lectin-like activities of TNF-α. 28. Use of an antibody or antisenεe peptide according to any of claims 23 to 26, characterized in that said antibody or said peptide inhibits the lectin-like activities of TNF-α. 29. Use of an antibody or antisenεe peptide according to any of claimε 23 to 26, characterized in that εaid antibody or said peptide stimulates the lectin-like activities of TNF-α. 30. Use of an antibody or antiεenεe peptide according to any of claims 23 to 26, characterized in that said antibody or said peptide inhibits the toxic activity of TNF-α. 31. Use of an antibody or antisenεe peptide according to any of claimε 23 to 26, characterized in that εaid antibody or said peptide modulates the inflammatory cytokineε inducing capacitieε of TNF-α. 32. Uεe of an antibody or antiεenεe peptide according to any of claimε 23 to 26, characterized in that εaid antibody or said peptide inhibits the inflammatory cytokines inducing capacitieε of TNF-α. 33. Uεe of an antibody or antiεenεe peptide according to any of claimε 23 to 26, characterized in that said antibody or said peptide modulateε the adheεion molecules inducing capacities of TNF-α. 34. Use of an antibody or antiεenεe peptide according to any of claimε 23 to 26, characterized in that said antibody or said peptide inhibits the adhesion molecules inducing capacitieε of TNF-α. 35. Uεe of an antibody or antisense peptide according to any of claimε 23 to 26, characterized in that εaid antibody or εaid peptide εtimulates the adhesion molecules inducing capacities of TNF-α. 36. Use of an antibody or antiεense peptide according to any of claims 23 to 26, characterized in that εaid antibody or εaid peptide inhibitε the metaεtaεiε promoting activity TNF-α. 37. Uεe of an antibody or antiεenεe peptide according to any of claimε 24 or 26, characterized in that εaid antibody or εaid peptide increases the half life time of TNF-α. 38. Cells transfected with a nucleic acid according to claim 18 coding for the TNF muteins according to any of claimε 1 to 17, said nucleic acid being inεerted into any suitable vector, with said cellε being preferably autologouε cellε derived from the patient (e.g. a cancer patient) to be treated with such compositions,* and with said vector-insert combination being constructed in such a way aε to allow continuouε expression of the TNF mutein at either a constant level, or at a level which can be modified, depending on the exact nature of the vector used to make the vector-insert combination. 39. Pharmaceutical composition containing as active substance transfected cells according to claim 38, in asεociation with a pharmaceutical acceptable vehicle. 40. Uεe of tranεfected cellε according to claim 38, for the preparation of a medicament for treating illnesseε εuch aε cancer.

Inventors: DE BAETSELIER PATRICK, FRANSEN LUCIE, LUCAS RUDOLPH, SABLON ERWIN, DE BAETSELIER, PATRICK, FRANSEN, LUCIE, LUCAS, RUDOLPH, SABLON, ERWIN
Applicants: BAETSELIER PATRICK DE, FRANSEN LUCIE, INNOGENETICS NV, LUCAS RUDOLPH, SABLON ERWIN, DE BAETSELIER, PATRICK, FRANSEN, LUCIE, LUCAS, RUDOLPH, N.V. INNOGENETICS S.A., SABLON, ERWIN
Application year: 1994
Main classification: C12N15
IPC codes: A61K38, C07K14, C12N15, C12P21