DNA SEQUENCES AND PLASMIDS FOR THE PREPARATION OF SUGAR BEET WITH CHANGED SUCROSE CONCENTRATION

Abstract: DNA sequences and plasmids are described, that by integration in a plant genome of sugar beet change the sucrose concentration, as well as transgenic plants that by introduction of the DNA sequences of the invention causes changes in sugar concentration.

Description: Title: DNA sequences and plasmids for the preparation of sugar beet with changed sucrose concentration Field of the invention The present invention relates to DNA sequences and plasmids, containing these DNA sequences, which by integration into the genome of a sugar-beet plant, changes the sugar metabolism of the plant to be changed. The invention also relates to transgenic plants formed with the help of these sequences. Sucrose is of central importance for the plant and serves many functions. For the long distance transport of photoassimilates and/or energy between various organs in μlcnts, -"""-rose is almost exclusively used. The sucrose, which is transported in a specific heterotrophic organ, determines the growth and the development of this organ. Thus it is known, e.g. from EP 442 592, that transgenic plants, in which the transport away of the sucrose from the exporting leaves is inhibited by expression of an apoplastic invertase, shows a strong reduction in the growth of e.g. roots or tubers in the case of potato plants. For tobacco plants, the principal importance of sucrose as the central function for the long distance transport of energy carriers within the plant is described (von Schaewen et al, 1990, EMBO J 9: 3033-3044). Further it is also known from EP 455 316 that DNA sequences present on plasmids, after introduction in a plant genome of a potato plant can affect the starch biosynthesis as well altering the amount and composition of the protein in the potato tubers. Whilst it has known that a reduction of the amount of sucrose imported in the heterotrophic organs, such as tubers and seeds, leads to loss of yield, it is not known whether an increase in the amount of sucrose in the photosynthetically active parts of the plant, mainly the leaves, leads to a better supply of heterotrophic organs and thus to an increase in yield. Besides sucrose and/or the hexoses, glucose and fructose, derived from sucrose, have the property of protection of plants against frost damage at low temperatures. Frost damage is one of the main limiting factors in agricultural productivity in the northern hemisphere. Temperatures below freezing lead to the formation of ice crystals. Since the growing ice crystals consist of pure water, water is abstracted from the cells as the temperature falls. This dehydration has at least two potential damaging results: 1. All dissolved substances within a cell are strongly concentrated and the cell contracts following the loss of water. Highly concentrated salts and organic acids lead to membrane damage. 2. With rehydration from dew, the previously contacted cells reexpand. The cell membrane also expands again. The volume expansion puts a heavy mechanical load on the membrane. It is thus clear that a freezing/dew cycle can lead to severe membrane damage of the cells and thus to damage to the plant. It thus appears worth trying to hinder the freezing. One possible strategy is the increased formation of os otically active substances in the cytosol of plant cells. This should lead to a lowering of the freezing point. Osmotically active substances include sucrose and/or the two hexoses derived from sucrose. The increased formation of sucrose and/or the two hexoses at low temperatures is desirable in the growing plant. Another situation can exist in the harvested parts of a plant, especially in storage. In relation to the economic aspects, sucrose thus possesses two especially important functions: 1 as the transport form for the distant transport of photoassi ilates, 2 as an osmotically active substance with the desirable activity of lowering the freezing point in intact, growing plants. The biosynthesis pathways for the formation of sucrose, either from the primary photosynthesis products (in the leaf) or by breakdown of starch (in the storage organs e.g. of potatoes) , are known. It is however, not known how and in what way changes of the carbohydrate concentration in sugar beet can be achieved since it is not possible to use even very similar genes such as for example genes that code for a sucrose synthase, ADP-glucose pyrophosphorylase or sucrose phosphate synthase of the potato with satisfactory success for the preparation of sugar beet with changed sucrose concentration. An exact analysis and determination of the DNA sequences or sequence fragments for the sugar beet is thus required. To change the sugar concentration in sugar beet, DNA sequences are now provided which code for the small and large subunit of the ADP glucose pyrophosphorylase, the sucrose synthase and the sucrose phosphate synthase of sugar beet (Seq. ID No 1-4) . These DNA sequences can be introduced into plasmids and in this way combined with steering elements for expression in eukaryotic cells. Such steering elements are on the one hand transcription promoters and on the other hand transcription terminators. Each plasmid comprises: a) a suitable promoter which ensures that the coding sequence meets a suitable time point or in the specified developments in the transgenic plant or in determined genes of transgenic plants; b) at least a coding sequence for sugar beet that i) is so coupled to the promoter that the formation of an RNA is allowed which is into a protein, whereby the protein demonstrates an enzymatic activity which leads to a change of the sucrose concentration in the plant, or ii) which is so coupled to the promoter that the coding part is read, which leads to the formation of a so-called anti-sense RNA which under-expresses the formation of the protein coded from an endogenous gene in the plant, that is involved in the sucrose biosynthesis; and c) A non-coding termination sequence that contains the signal for the termination and polyadenylation of the transcript. The coding sequences named under b) are the sequences that code for the large and small subunit of the ADP glucose pyrophosphorylase, for the sucrose phosphate synthase and for the sucrose synthase of sugar beet. 5 The large subunit of the ADP-glucose-pyrophosphorylase has the following nucleotide sequence (Seq. ID No. 1) : CAAAAGAAAA ACTTCCCATT TCTACTTCTT TGCACAATAT AATTTCCCAC 0050 CAATTTTTCT TTAAATTTCT CACTTTCATT TAATCAGTTT TCAGCAACAT 0100 TCTGATACTC GACAACCCAC TTTCTGTTCT CCCAAGATTC CAAACCTCTG 0150 ATTCTCATTC CACTAATATT TTTGCTTATT TTTTTTCTGG ATTTAAAGAA 0200 AAGCT ATG GAT GCA AGT GCA GCA GCC ATA AAT GTC AAT GCC CAT 0243 Met Asp Ala Ser Ala Ala Ala lie Asn Val Asn Ala His 5 10 TTA ACA GAA GTT GGA AAG AAA CGT TTT TTA GGA GAG AGA ATC AGT 0288 Leu Thr Glu Val Gly Lys Lys Arg Phe Leu Gly Glu Arg lie Ser 15 20 25 CAA AGT TTG AAG GGT AAA GAT CTG AGA GCT CTG TTT TCA AGA ACT 0333 Gin Ser Leu Lys Gly Lys Asp Leu Arg Ala Leu Phe Ser Arg Thr 30 35 40 GAG AGC AAG GGT AGA AAT GTC AAT AAA CCT GGG GTT GCA TTT TCT 0378 Glu Ser Lys Gly Arg Asn Val Asn Lys Pro Gly Val Ala Phe Ser 45 ' 50 55 GTT CTC ACC TCA GAT TTT AAT CAA AGT GTT AAA GAA TCT TTG AAA 0423 Val Leu Thr Ser Asp Phe Asn Gin Ser Val Lys Glu Ser Leu Lys 60 65 70 TAT GAG CCA GCA TTA TTT GAA TCT CCA AAA GCT GAC CCA AAA AAT 0468 Tyr Glu Pro Ala Leu Phe Glu Ser Pro Lys Ala Asp Pro Lys Asn 75 80 85 GTG GCT GCA ATT GTG CTG GGT GGT GGT GCT GGG ACT CGC CTC TTT 0513 Val Ala Ala lie Val Leu Gly Gly Gly Ala Gly Thr Arg Leu Phe 90 95 100 CCT CTT ACT AGC AGG AGA GCT AAG CCA GCA GTG CCA ATT GGA GGG 055. Pro Leu Thr Ser Arg Arg Ala Lys Pro Ala Val Pro lie Gly Gly 105 110 115 TGT TAC AGG CTG ATT GAT GTG CCT ATG AGC AAC TGC ATC AAC AGT 0603 Cys Tyr Arg Leu lie Asp Val Pro Met Ser Asn Cys lie Asn Ser 120 125 130 GGC ATT AGA AAG ATT TTC ATT CTT ACC CAG TTC AAT TCG TTT TCG 0648 Gly lie Arg Lys lie Phe lie Leu Thr Gin Phe Asn Ser Phe Ser 135 140 145 CTT AAT CGT CAT CTT GCT CGA ACC TAT AAT TTT GGA GAT GGT GTG 0693 Leu Asn Arg His Leu Ala Arg Thr Tyr Asn Phe Gly Asp Gly Val 150 155 160 AAT TTT GGG GAT GGC TTT GTG GAG GTT TTT GCT GCT ACA CAA ACA 0738 Asn Phe Gly Asp Gly Phe ' Val Glu Val Phe Ala Ala Thr Gin Thr 165 170 175 CCT GGA GAA TCA GGA AAG AAA TGG TTC CAG GGC ACC GCT GAT GCA 0783 Pro Gly Glu Ser Gly Lys Lys Trp Phe Gin Gly Thr Ala Asp Ala 180 185 190 GTA AGA CAG TTT TTC TGG GCA TTT GAG GAT TCC AAA TCC AAG GAT 0828 Val Arg Gin Phe Phe Trp Ala Phe Glu Asp Ser Lys Ser Lys Asp 195 200 205 GTC GAG CAT ATA GTT ATT TTA TCC GGT GAT CAT CTT TAC CGA ATG 0873 Val Glu His lie Val lie Leu Ser Gly Asp His Leu Tyr Arg Met 210 215 220 GAT TAC ATG AGT TTT TGG CAG AAG CAC ATT GAC ACC AAT GCT GAT 0918 Asp Tyr Met Ser Phe Trp Gin Lys His lie Asp Thr Asn Ala As 225 230 235 ATT ACA GTG TCA TGC ATA CCC ATG GAT GAC AGC CGT GCA TCG GAT 0963 lie Thr Val Ser Cys lie Pro Met Asp Asp Ser Arg Ala Ser Asp 240 245 250 TAT GGG CTG ATG AAG ATT GAT CAC ACT GGA CGC ATT GTC CAT TTT 1008 Tyr Gly Leu Met Lys lie Asp His Thr Gly Arg lie Val His Phe 255 260 265 GCA GAA AAA CCC AAG GGT ' TCT GAT CTA ACA GCA ATG CAA GTA GAT 1053 Ala Glu Lys Pro Lys Gly Ser Asp Leu Thr Ala Met Gin Val Asp 270 275 280 ACA ACT GTT CTT GGG CTC TCT GAC CTT GAA GCT ATG TCA AAT CCA 1098 Thr Thr Val Leu Gly Leu Ser Asp Leu Glu Ala Met Ser Asn Pro 285 290 295 TAT ATT GCA TCA ATG GGT GTT TAT GTC TTT CGA ACG GAT GTT CTT 1143 Tyr lie Ala Ser Met Gly Val Tyr Val Phe Arg Thr Asp Val Leu 300 305 310 ATG GAG CTT CTC AAT CGA AAA TAC CCT TCA AGC AAT GAT TTT GGC 1188 Met Glu Leu Leu Asn Arg Lys Tyr Pro Ser Ser Asn Asp Phe Gly 315 320 325 TCT GAG ATT ATT CCT TCA GCT GTA GGA GAG TCT AAT GTT CAG GCA 1233 Ser Glu lie lie Pro Ser Ala Val Gly Glu Ser Asn Val Gin Ala 330 335 340 TAT CTA TTT AAT GAC TAC TGG GAG GAT ATC GGA ACC ATA AAG TCT 1278 Tyr Leu Phe Asn Asp Tyr Trp Glu Asp lie Gly Thr lie Lys Ser 345 350 355 TTC TTT GAT TCC AAT TTG GCC CTT ACA CAA CAG CCT CCC AAG TTT 1323 Phe Phe Asp Ser Asn Leu Ala Leu Thr Gin Gin Pro Pro Lys Phe 360 365 370 GAA TTC TAC GAT CCA AAA ' ACA CCT TTT TAT ACA TCT GCA AGA TTT 1368 Glu Phe Tyr Asp Pro Lys Thr Pro Phe Tyr Thr Ser Ala Arg Phe 375 380 385 CTG CCT CCT ACA AAA GTC GAC AGG TGC AAG ATT GTC GAT TCC ATT 1413 Leu Pro Pro Thr Lys Val Asp Arg Cys Lys lie Val Asp Ser lie 390 395 400 GTA TCC CAT GGT TGT TTT CTA CAG GAG TCT AGC ATC CAA CAT TCC 1458 Val Ser His Gly Cys Phe Leu Gin Glu Ser Ser lie Gin His Ser 405 410 415 ATT GTT GGT GTT CGC TCA AGA TTA GAG TCC GGG GTT GAG TTC CAG 1503 lie Val Gly Val Arg Ser Arg Leu Glu Ser Gly Val Glu Phe Gin 420 425 430 GAC ACC ATG ATG ATG GGC GCA GAT TAC TAT CAA ACT GAA TCA GAA .3.48 Asp Thr Met Met Met Gly Ala Asp Tyr Tyr Gin Thr Glu Ser Glu 435 440 445 ATT GCT TCT CTG CTT GCT GAG GGA AAG GTT CCT GTT GGT GTC GGA 159: lie Ala Ser Leu Leu Ala Glu Gly Lys Val Pro Val Gly Val Gly 450 455 460 CAG AAT ACC AAA ATA AAG AAT TGC ATA ATT GAC AAG AAC GCC AAA 1638 Gin Asn Thr Lys lie Lys Asn Cys lie lie Asp Lys Asn Ala Lys 465 470 475 ATT GGA AAA GAT GTG GTA ATC GCA AAC ACG GAT GGT GTT GAG GAA 1683 lie Gly Lys Asp Val Val lie Ala Asn Thr Asp Gly Val Glu Glu 480 485 490 GCA GAT AGA CCA AAT GAA GGC TTT TAC ATC AGG TCG GGC ATT ACC 1728 Ala Asp Arg Pro Asn Glu Gly Phe Tyr lie Arg Ser Gly lie Thr 495 500 505 ATC ATT TTG AAG AAC GCA ACC ATA CAA GAC GGT CTT GTG ATT TAG 1773 lie lie Leu Lys Asn Ala Thr lie Gin Asp Gly Leu Val lie End 510 515 520 ATTTAATCAT AACCTCATTA GAAAGAAATA ATTTTGCATG ATTTCCTTTT 1823 CATGTAACCT AAACTGGCTA AACCACGAGG TTTTCTCATC TGTATATATA 1873 ATATGTCTAT AACTATGGAT AATCTTAATA AAAAAAAAAA AAAAAAAAAA 1923 1924 The small subunit of the ADP-glucose-pyrophosphalaεe has the following nucleotide sequence (Seq. ID No. 2) : GG ATA ACT GTG CCA TCA ACC TCC TCA AAG AAC CTC CAA AAT AGC 0044 lie Thr Val Pro Ser Thr Ser Ser Lys Asn Leu Gin Asn Ser 5 10 CTC GCA TTC TCC TCT TCT TCT CTC TCC GGC GAC AAA ATT CAA ACG 0089 Leu Ala Phe Ser Ser Ser Ser Leu Ser Gly Asp Lys lie Gin Thr 15 20 25 ACG TCA TTT CTC AAC CGC CGA TAT TGT AGA ATC TCT TCT AGA GCT 0134 Thr Ser Phe Leu Asn Arg Arg Tyr Cys Arg lie Ser Ser Arg Ala 30 35 40 CCG ATT GTT GTC TCT CCC AAA GCT GTT TCT GAT TCT AAG AAT TCG 0179 Pro lie Val Val Ser Pro Lys Ala Val Ser Asp Ser Lys Asn Ser 45 50 55 CAG ACT TGT CTT GAC CCT GAA GCC AGC CGT AGT GTT CTT GGT ATT 0224 Gin Thr Cys Leu Asp Pro Glu Ala Ser Arg Ser Val Leu Gly lie 60 65 70 ATA CTT GGA GGT GGT GCT GGT ACA CGT CTT TAC CCG TTG ACT AAG 0269 lie Leu Gly Gly Gly Ala Gly Thr Arg Leu Tyr Pro Leu Thr Lys 75 80 85 AAG AGA GCC AAA CCA GCC GTG CCA CTC GGT GCT AAT TAT AGG CTT 0314 Lys Arg Ala Lys Pro Ala Val Pro Leu Gly Ala Asn Tyr Arg Leu 90 95 100 ATT GAT ATC CCA GTG AGC AAT TGT TTG AAC AGT AAT ATT TCC AAA 0359 lie Asp lie Pro Val Ser Asn Cys Leu Asn Ser Asn lie Ser Lys 105 110 110 ATA TAT GTT CTT ACA CAA TTC AAT TCT GCT TCT CTG AAT CGT CAT 0404 lie Tyr Val Leu Thr Gin Phe Asn Ser Ala Ser Leu Asn Arg His 115 120 125 CTT TCG CGG GCA TAT GCT AGC AAC ATG GGA GGA TAC AAA AAT GAG 0449 Leu Ser Arg Ala Tyr Ala Ser Asn Met Gly Gly Tyr Lys Asn Glu 130 135 140 GGG TTT GTA GAA GTT CTT GCT GCT CAG CAA AGT CCA GAG AAT CCA 0494 Gly Phe Val Glu Val Leu Ala Ala Gin Gin Ser Pro Glu Asn Pro 145 150 155 AAC TGG TTT CAG GGT ACA GCT GAT GCT GTT AGG CAA TAT CTG TGG 0539 Asn Trp Phe Gin Gly Thr Ala Asp Ala Val Arg Gin Tyr Leu Trp 160 165 170 CTT TTC GAA GAG CAC AAT GTT CTT GAG TAC TTG ATT CTT GCT GGT 0584 Leu Phe Glu Glu His Asn Val Leu Glu Tyr Leu lie Leu Ala Gly 175 180 185 GAC CAT TTG TAT CGA ATG GAT TAT GAA AGA TTT GTC CAA GCT CAC 0629 Asp His Leu Tyr Arg Met Asp Tyr Glu Arg Phe Val Gin Ala His 190 195 200 AGA GAA ACT GAT GCA GAC ATT ACT GTT GCT GCA TTG CCA ATG GAT 0674 Arg Glu Thr Asp Ala Asp lie Thr Val Ala Ala Leu Pro Met __sp 205 210 215 GAA AAG CGT GCT ACT GCA TTT GGT TTG ATG AAA ATT GAT GAA GAA 0719 Glu Lys Arg Ala Thr Ala Phe Gly Leu Met Lys lie Asp Glu Glu 220 225 230 GGA AGA ATT ATT GAG TTT GCC GAG AAA CCG AAA GGA GAA CAA TTG 0764 Gly Arg lie lie Glu Phe Ala Glu Lys Pro Lys Gly Glu Gin Leu 235 240 245 AAA GCT ATG AAG GTT GAT ACC ACA ATC CTG GGT CTG GAC GAT GAG 0809 Lys Ala Met Lys Val Asp Thr Thr lie Leu Gly Leu Asp Asp Glu 250 255 260 AGA GCA AAA GAA ATG CCA TTC ATA GCC AGC ATG GGC ATA TAT GTT 0854 Arg Ala Lys Glu Met Pro Phe lie Ala Ser Met Gly lie Tyr Val 265 270 275 ATT AGC AAA GAT GTA ATG CTT AAT CTG CTT CGG GAG CAA TTT CCT 0899 lie Ser Lys Asp Val Met Leu Asn Leu Leu Arg Glu Gin Phe Pro 280 285 290 GGT GCT AAT GAT TTT GGA AGT GAA GTT ATT CCA GGC GCC ACT TCC 0944 Gly Ala Asn Asp Phe Gly Ser Glu Val lie Pro Gly Ala Thr Ser 295 300 305 ATA GGG TTG AGA GTC CAA GCT TAT TTG TAT GAT GGT TAC TGG GAG 0989 lie Gly Leu Arg Val Gin Ala Tyr Leu Tyr Asp Gly Tyr Trp Glu 310 315 320 GAT ATT GGT ACC ATT GAA GCT TTT TAC AAT GCT AAC TTG GGA ATC 1034 Asp lie Gly Thr lie Glu Ala Phe Tyr Asn Ala Asn Leu Gly lie 325 330 335 ACC AAA AAG CCG GTG CCA GAT TTT AGC TTC TAT GAT CGT TCA TCT 1079 Thr Lys Lys Pro Val Pro Asp Phe Ser Phe Tyr Asp Arg Ser Ser 340 345 350 CCA ATT TAT ACA CAA CCT CGG TAT TTG CCT CCT TCA AAG ATG CTT 1124 Pro lie Tyr Thr Gin Pro Arg Tyr Leu Pro Pro Ser Lys Met Leu 355 360 365 GAT GCT GAT ATA ACT GAC AGC GTC ATC GGT GAA GGC TGT GTT ATT 1169 Asp Ala Asp lie Thr Asp Ser Val lie Gly Glu Gly Cys Val lie 370 375 380 AAG AAC TGT AAG ATT CAT CAT TCT GTT ATC GGA CTT CGA TCT TGT 1214 Lys Asn Cys Lys lie His His Ser Val lie Gly Leu Arg Ser Cys 385 390 395 ATC TCG GAG GGT GCA ATC ATT GAG GAC ACA CTG TTG ATG GGA GCT 1259 lie Ser Glu Gly Ala lie lie Glu Asp Thr Leu Leu Met Gly Ala 400 405 410 GAT TAT TAT GAG ACT GAT GCT GAT CGG AAA TTC CTG GCT GCT AAG 1304 Asp Tyr Tyr Glu Thr Asp Ala Asp Arg Lys Phe Leu Ala Ala Lye 415 420 425 GGT AGT GTA CCT ATT GGA ATT GGG AAT GCA CGT ATT GGG GAT GAT 1349 Gly Ser Val Pro lie Gly lie Gly Asn Ala Arg lie Gly Asp Asp 430 435 440 GTC AAG ATT ATC AAC AGT GAC AAT GTA CAA GAA GCA GCA AGA GAA 1394 Val Lys lie lie Asn Ser Asp Asn Val Gin Glu Ala Ala Arg Glu 445 450 455 ACA GAC GGA TAC TTC ATA AAG AGC GGA ATA GTC ACT ATA ATC AAG 1439 Thr Asp Gly Tyr Phe lie Lys Ser Gly lie Val Thr lie lie Lys 460 465 470 GAC GCC ATG ATT CCA AGT GGA ACT GTA ATC TAG AAATGGAGCA 1482 Asp Ala Met lie Pro Ser Gly Thr Val lie End 475 480 485 TATAATAAAT ATCACTGCCT ATTTACAGTA ' CCTATCTGAG TCTCCCACCA 1532 TGACCCTTTG ATTCAATCTT TTAGTTATGT AAATATTTTT GGCTTTTGCG 1582 ATTTTGCCAT AAATTTGAAG AAGCGAGGAT TCAGGGACGA TAGTGCTATG 1632 AATTGGAAGA AAGGATTTGG GGGATATCTT TGTAAAGACA TTTTGACTAC 1682 TGGGCACTAA AAATTTGGTA ATGCTATACC AAAATATATA AAAAGATCTT 1732 GCTGGGTTTT GGTAAAAAAA AAAAAAAAAA A 1763 The sucrose phosphate - synthase has the following nucleotide sequence (Seq. ID No. 3) : GGGCTGCAGG GAAGCTCTGA ACTTCAAAA ATG GCG GGA AAT GAT 0044 Met Ala Gly Asn Asp 5 TGG ATA AAC AGT TAT TTA GAG GCA ATT CTG GAT GTG GGT CCA GGA 0089 Trp lie Asn Ser Tyr Leu Glu Ala lie Leu Asp Val Gly Pro Gly 10 15 20 CTT GAT GAT GCA AAA TCA TCT TTG CTT TTG AGA GAA AGA GGC AGG 0134 Leu Asp Asp Ala Lys Ser Ser Leu Leu Leu Arg Glu Arg Gly Arg 25 30 35 TTT AGT CCT ACT CGT TAC TTT GTT GAA GAA GTT ATC ACT GGT TTT 0179 Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu Val lie Thr Glv Phe 40 45 50 GAT GAA ACC GAC CTT CAT CGT TCA TGG GTT CGG GCA CAA GCA ACA 0224 Asp Glu Thr Asp Leu His Arg Ser Trp Val Arg Ala Gin Ala Thr 55 60 65 AGG AGT CCT CAA GAG AGG AAT ACT AGA TTG GAG AAC ATG TGT TGG 0269 Arg Ser Pro Gin Glu Arg Asn Thr Arg Leu Glu Asn Met Cys Trp 70 75 80 AGA ATT TGG AAT TTG GCT CGT CAG AAG AAG CAG CTT GAG AAT GAA 0314 Arg lie Trp Asn Leu Ala Arg Gin Lys Lys Gin Leu Glu Asn Glu 85 90 95 GAA GCT CAG CGG AAG ACA AAA CGT CGT ATG GAG CTT GAG AGG GGT 0359 Glu Ala Gin Arg Lys Thr Lys Arg Arg Met Glu Leu Glu Arg Gly 100 105 110 CGT CGA GAA GCA ACT GCT GAT ATG TCG GAG GAC TTA TCA GAA GGC 0404 Arg Arg Glu Ala Thr Ala Asp Met Ser Glu Asp Leu Ser Glu Gly 115 120 125 GAA AAG GAC ATT TCA GCT CAT GGT GAT AGC ACC CGT CCT AGA TTG 0449 Glu Lys Asp lie Ser Ala His Gly Asp Ser Thr Arg Pro Arg Leu 130 135 140 CCA AGA ATA AAT TCT CTT GAT GCT ATG GAG .ACA TGG ATT AGT CAA 0494 Pro Arg lie Asn Ser Leu Asp Ala Met Glu Thr Trp lie Ser Gin 145 150 155 CAA AAG GAA AAA AAA CTC TAC CTT GTT TTG ATA AGT CTT CAT GGT 0539 Gin Lys Glu Lys Lys Leu Tyr Leu Val Leu lie Ser Leu His Gly 160 165 170 TTG ATA CGA GGT GAA AAC ATG GAA CTT GGC CGT GAT TCT GAT ACT 0584 Leu lie Arg Gly Glu Asn Met Glu Leu Gly Arg Asp Ser Asp Thr 175 180 185 GGT GGT CAG GTT AAG TAT GTG GTT GAG CTT GCA AGG GCT CTA GGT 0629 Gly Gly Gin Val Lys Tyr Val Val Glu Leu Ala Arg Ala Leu Gly 190 195 200 TCG ATG CCA GGT GTT TAT AGA GTT GAT TTG CTA ACT AGG CAA GTT 0674 Ser Met Pro Gly Val Tyr Arg Val Asp Leu Leu Thr Arg Gin Val 205 210 215 TCA TCT CCT GAC GTG GAT TGG AGT TAT GGG GAG CCT ACT GAG ATG 0719 Ser Ser Pro Asp Val Asp Trp Ser Tyr Gly Glu Pro Thr Glu Met 220 225 230 CTG AAT CCA AGG GAT TCC AAT GGT TTT GAT GAT GAT GAT GAT GAA 0764 Leu Asn Pro Arg Asp Ser Asn Gly Phe Asp Asp Asp Asp Asp Glu 235 240 245 ATG GGA GAG AGT AGT GGT GCT TAC ATT GTT CGT ATA CCA TTT GGG 0809 Met Gly Glu Ser Ser Gly Ala Tyr lie Val Arg lie Pro Phe Gly 250 255 260 CCG AGG GAT AAG TAT ATC GCA AAA GAA GAG CTT TGG CCC TAT ATT 0854 Pro Arg Asp Lys Tyr lie Ala Lys Glu Glu Leu Trp Pro Tyr lie 265 270 275 CCT GAA TTT GTT GAT GGT GCT CTA AAC CAC ATA GTT CAA ATG TCC 0899 Pro Glu Phe Val Asp Gly Ala Leu Asn His lie Val Gin Met Ser 280 285 290 AAA GTT TTA GGT GAG CAA ATT GGT AGC GGG GAA ACA GTT TGG CCA 0944 Lys Val Leu Gly Glu Gin lie Gly Ser Gly Glu Thr Val Trp Pro 295 300 305 GTT GCC ATT CAT GGA CAT TAT GCT GAT GCT GGT GAT TCT GCT GCT 0989 Val Ala lie His Gly His Tyr Ala Asp Ala Gly Asp Ser Ala Ala 310 315 320 CTT CTT TCT GGT GGC CTA AAT GTT CCA ATG CTT TTA ACG GGG CAT 1034 Leu Leu Ser Gly Gly Leu Asn Val Pro Met Leu Leu Thr Gly His 325 330 335 TCT CTT GGC CGA GAC AAG TTA GAG CAG CTC CTC AAA CAG GGT CGA 1079 Ser Leu Gly Arg Asp Lys Leu Glu Gin Leu Leu Lys Gin Gly Arg 340 345 350 ATG TCT AAA GAT GAC ATA AAC AAT ACA TAC AAA ATA ATG CGT AGG 1124 Met Ser Lys Asp Asp lie Asn Asn Thr Tyr Lys lie Met Arg Arg 355 360 365 ATA GAA GCC GAA GAG TTA ' TCA CTT GAT GCC TCT GAG ATA GTC ATA 1169 lie Glu Ala Glu Glu Leu Ser Leu Asp Ala Ser Glu lie Val lie 370 375 380 ACT AGT ACA AGA CAA GAA ATA GAA GAG CAA TGG CAC CTC TAT GAT 1214 Thr Ser Thr Arg Gin Glu lie Glu Glu Gin Trp His Leu Tyr Asp 385 390 395 GGG TTT GAT CCT GTG CTA GAA CGT AAA CTC CGT GCT AGG ATG AAG 1259 Gly Phe Asp Pro Val Leu Glu Arg Lys Leu Arg Ala Arg Met Lys 400 405 410 CGT GGT GTA AGC TGT TAT GGA AGG TTC ATG CCC CGG ATG GTT GTT 1304 Arg Gly Val Ser Cys Tyr Gly Arg Phe Met Pro Arg Met Val Val 415 420 425 ATT CCT CCT GGA ATG GAA TTC AAT CAT ATT GTT CCA CAT GAG GGT 1349 lie Pro Pro Gly Met Glu Phe Asn His lie Val Pro His Glu Gly 430 435 440 GAT ATG GAT GGT GAA ACA GAA GAA ACT GAA GAG CAT CCT ACA TCA 1394 Asp Met Asp Gly Glu Thr Glu Glu Thr Glu Glu His Pro Thr Ser 445 450 455 CCT GAT CCA CCT ATC TGG GCT GAG ATT ATG CGC TTC TTT TCT AAA 1439 Pro Asp Pro Pro lie Trp Ala Glu lie Met Arg Phe Phe Ser Lys 460 465 470 CCA AGG AAG CCA ATG ATA ' CTT GCC CTT GCT AGG CCT GAC CCG AAG 1484 Pro Arg Lys Pro Met lie Leu Ala Leu Ala Arg Pro Asp Pro Lys 475 480 485 AAG AAT ATC ACG ACT TTG GTC AAA GCA TTT GGA GAA TGC CGT CCA 1529 Lys Asn lie Thr Thr Leu Val Lys Ala Phe Gly Glu Cys Arg Pro 490 495 500 CTA AGG GAG CTA GCT AAT CTT ACT CTT ATA ATG GGT AAC CGA GAT 1574 Leu Arg Glu Leu Ala Asn Leu Thr Leu lie Met Gly Asn Arg Asp 505 510 515 GGT ATT GAC GAG ATG TCA AGC ACC AGT TCT TCA GTT CTC CTG TCA 1619 Gly lie Asp Glu Met Ser Ser Thr Ser Ser Ser Val Leu Leu Ser 520 525 530 GTG CTT AAG CTA ATT GAT CAA TAC GAC CTT TAT GGT CAA GTA GCA 1664 Val Leu Lys Leu lie Asp Gin Tyr Asp Leu Tyr Gly Gin Val Ala 535 540 545 TAC CCC AAA CAT CAC AAG CAA GCT GAT GTT CCT GAG ATT TAT CGT 1709 Tyr Pro Lys His His Lys Gin Ala Asp Val Pro Glu lie Tyr Arg 550 555 600 TTG GCA GCA AAG ACA AAG GGA GTC TTT ATT AAT CCA GCT TTT ATT 1754 Leu Ala Ala Lys Thr Lys Gly Val Phe lie Asn Pro Ala Phe lie 605 610 615 GAG CCA TTT GGG CTG ACT CTA ATA GAG GCA GCA GCT CAT GGT TTA 1799 Glu Pro Phe Gly Leu Thr Leu lie Glu Ala Ala Ala His Gly Leu 620 625 630 CCG ATG GTT GCT ACG AAA AAT GGA GGC CCT GTT GAT ATC CAG AGG 1844 Pro Met Val Ala Thr Lys Asn Gly Gly Pro Val Asp lie Gin Arg 635 640 645 GTC CTT GAT AAT GGT CTT CTT GTG GAT CCT CAT GAG CAG CAG TCT 1889 Val Leu Asp Asn Gly Leu Leu Val Asp Pro His Glu Gin Gin Ser 650 655 660 ATT GCT ACT GCT TTG CTG AAG CTT GTT GCT GAT AAG CAA CTA TGG 1934 lie Ala Thr Ala Leu Leu Lys Leu Val Ala Asp Lys Gin Leu Trp 665 670 675 ACA AAA TGC CAG CAA AAT GGA CTG AAA AAT ATT CAT CTC TAC TCT 1979 Thr Lys Cys Gin Gin Asn Gly Leu Lys Asn He His Leu Tyr Ser 680 685 690 TGG CCA GAG CAT TCG AAG ACA TAC CTA TCT CGA ATA GCC AGT TCG 2024 Trp Pro Glu His Ser Lys Thr Tyr Leu Ser Arg He Ala Ser Ser 695 700 705 AGA CAA AGG CAA CCA CAG TGG CAA AGA AGT AGT GAT GAA GGG CTT 2069 Arg Gin Arg Gin Pro Gin Trp Gin Arg Ser Ser Asp Glu Gly Leu 710 715 720 GAC AAT CAA GAG CCT GAA TCT CCA AGT GAT TCT TTA AGA GAT ATA 2114 Asp Asn Gin Glu Pro Glu Ser Pro Ser Asp Ser Leu Arg Asp He 725 730 735 AAG GAT ATA TCT CTA AAC CTT GAA GTT CTC GTT AGA CCG GAG AAA 2159 Lys Asp He Ser Leu Asn Leu Glu Val Leu Val Arg Pro Glu Lys 740 745 750 AGG GTG AAG ACG TTG AAA ATC TTG GGA TTG ATG ACA AAA GCA AAT 2204 Arg Val Lys Thr Leu Lys He Leu Gly Leu Met Thr Lys Ala Asn 755 760 765 TCG AGA ATG CTG TTA TGT TCA TGG TCT AAT GGT GTC CAT AAG ATG 2249 Ser Arg Met Leu Leu Cys Ser Trp Ser Asn Gly Val His Lys Met 770 775 780 CTT CGG AAG GCT CGG TTC TCT GAC AAA GTA GAT CAG GCT TCT AGT 2294 Leu Arg Lys Ala Arg Phe Ser Asp Lys Val A_-p Gin __.= Ser Ser 785 790 795 AAA TAT CCA GCA TTT AGG AGG AGA AAA CTT ATA TAT GTT ATT GCT 2339 Lys Tyr Pro Ala Phe Arg Arg Arg Lys Leu He Tyr Val He Ala 800 805 810 GTA GAC GGG GAT TAT GAA GAT GGA CTT TTT GAT ATT GTT CGG AGG 2384 Val Asp Gly Asp Tyr Glu Asp Gly Leu Phe Asp He Val Arg Arg 815 820 825 ATA TTT GAT GCT GCT GGC AAG GAG AAG ATT GAA GGT TCC ATC GGG 2429 He Phe Asp Ala Ala Gly Lys Glu Lys He Glu Gly Ser He Gly 830 835 840 TTT ATA TTG TCA ACA TCC TAT TCT ATG CCC GAA ATT CAG AAC TAT 2474 Phe He Leu Ser Thr Ser Tyr Ser Met Pro Glu He Gin Asn Tyr 845 850 855 TTG CTA TCA AAA GGC TTC AAT CTT CAT GAT TTT GAT GCA TAT ATA 2519 Leu Leu Ser Lys Gly Phe Asn Leu His Asp Phe Asp Ala Tyr He 860 865 870 TGC AAC AGT GGG AGT GAG TTG TAC TAT TCA TCT TTG AAC TCA GAG 2564 Cys Asn Ser Gly Ser Glu Leu Tyr Tyr Ser Ser Leu Asn Ser Glu 875 880 885 GAG AGT AAT ATT ATA GCA GAT TCA GAT TAC CAT TCA CAC ATA GAG 2609 Glu Ser Asn He He Ala Asp Ser Asp Tyr His Ser His He Glu 890 895 900 TAC AGA TGG GGT GGA GAA GGC CTT AGA AGG ACT TTG CTT CGC TGG 2654 Tyr Arg Trp Gly Gly Glu Gly Leu Arg Arg Thr Leu Leu Arg Trp 905 910 915 GCA GCT TCC ATC ACA GAA AAA AAT GGT GAA AAC GAA GAA CAG GTT 2699 Ala Ala Ser He Thr Glu Lys Asn Gly Glu Asn Glu Glu Gin Val 920 925 930 ATT ACT GAA GAT GAA GAA GTT TCT ACG GGT TAT TGC TTT GCG TTT 2744 He Thr Glu Asp Glu Glu Val Ser Thr Gly Tyr Cys Phe Ala Phe 935 940 945 AAA ATA AAG AAC CAA AAT AAG GTT CCC CCT ACG AAG GAG CTC CGC 2789 Lys He Lys Asn Gin Asn Lys Val Pro Pro Thr Lys Glu Leu Arg 950 955 960 AAG TCA ATG AGG ATT CAA GCT CTT CGT TGC CAT GTG ATT TAC TGT 2834 Lys Ser Met Arg He Gin Ala Leu Arg Cys His Val He Tyr Cys 965 970 975 CAG AAC GGA TCT AAA ATG AAT GTG ATT CCA GTA CTA GCA TCC CGT 2879 Gin Asn Gly Ser Lys Met Asn Val He Pro Val Leu Ala Ser Arg 980 985 990 TCT CAA GCC CTC AGG TAT CTT TAT GTT CGT TGG GGA GTT GAG TTG 2924 Ser Gin Ala Leu Arg Tyr Leu Tyr Val Arg Trp Gly Val Glu Leu 995 1000 1005 TCG AAG ATG GTT GTC TTT GTT GGA GAA TGT GGT GAC ACA GAT TAT 2969 Ser Lys Met Val Val Phe Val Gly Glu Cys Gly Asp Thr Asp Tyr 1010 1015 1020 GAA GGC TTG CTT GGC GGG GTC CAT AAA ACC GTA ATA CTG AAG GGA 3014 Glu Gly Leu Leu Gly Gly Val His Lys Thr Val He Leu Lys Gly 1025 1030 1035 GTC TCC AAC ACT GCT TTA AGG TCT CTC CAT GCC AAC AGA AGT TAC 3059 Val Ser Asn Thr Ala Leu Arg Ser Leu His Ala Asn Arg Ser Tyr 1040 1045 1050 CCT CTT TCA CAT GTC GTG TCG CTT GAC AGC CCC AAT ATT GGC GAG 3104 Pro Leu Ser His Val Val Ser Leu Asp Ser Pro Asn He Gly Glu 1055 1060 1065 GTG AGC AAA GGG TGC AGC AGC TCC GAG ATC CAG TCC ATC GTC ACA 3149 Val Ser Lys Gly Cys Ser Ser Ser Glu He Gin Ser He Val Thr 1070 1075 1080 AAA CTC TCC AAA GCT TAA TCAGATATCT GCTGCTTTCT TTTGGGTAAG 3197 Lys Leu Ser Lys Ala End 1085 CAAGGTTTCA TCTTATATGA TTATATCATA AGATACTATA TAAGCACCTT 3247 ATTGGTAAGT CAGTCCCATA ATAATAATGT ACTTCAGAAC CACAATACTT 3297 AAAAGTTGGT TCAGTAGTGA TTAGTCTCAT AATAATCATA TAATTACACA 3347 TCCGCTGTTA ACTAGTGGTA ATATCTAAGC TCAACAATAA AGATGTAAAA 3397 TGCTAGTATG GAAATGAATT GCTAGCTGTT GATCTCTTTC CCTTTATTCT 3447 GTATTATTTC TTTCCTCATC TCATGTAAAA ACAATTTTCT GAAGGTGTAC 3497 AGTTTTTTCC CCTTATATAT CTGTATTATT TCTACTATTT TTTGTTTGTA 3547 AGAATATCCT CTCATCGAGG AGTGATAATT AAATAACCGG CTTGCTAAAT 3597 ATAAAGCTTA TTCGAGTTAA AAAAAAAAAA AAAAAAAA 3635 The sucrose-synthase has the following nucleotide sequence (Seq. ID No. 4) : CT GCA GGA GGG AAA CAA ATT CTT AGC GAT GGC CCG TTT AGC GAA 0044 Ala Gly Gly Lys Gin He Leu Ser Asp Gly Pro Phe Ser Glu 5 10 GTT CTT AGG TCT GCT CAG GAA GCA ATA GTT GTT CCT CCC TTT GTT 0089 Val Leu Arg Ser Ala Gin Glu Ala He Val Val Pro Pro Phe Val 15 20 25 GCT ATA GCA GTC CGT CCA AGA CCT GGA GTT TGG GAA TAT GTT CGT 0134 Ala He Ala Val Arg Pro Arg Pro Gly Val Trp Glu Tyr Val Arg 30 35 40 GTT AAT GTC TCT GAA CTG AAT GTG GAG CAG CTA ACT GTG TCT GAG 0179 Val A__n Val Ser Glu Leu Asn Val Glu Gin Leu Thr Val Ser Glu 45 50 55 TAT CTC CAT TTC AAG GAA GAA CTT GTG GAT GGA AAG GCT GAT GAC 0224 Tyr Leu His Phe Lys Glu Glu Leu Val Asp Gly Lys Ala Asp Asp 60 65 70 CAC TAT GTG CTT GAG CTT GAT TTC GAG CCT TTT AAT GAA TCA GTT 0269 His Tyr Val Leu Glu Leu Asp Phe Glu Pro Phe Asn Glu Ser Val 75 80 85 CCA CGT CCA ACT CGC TCT TCA TCA ATT GGT AAT GGT GTT CAG TTC 0314 Pro Arg Pro Thr Arg Ser Ser Ser He Gly Asn Gly Val Gin Phe 90 95 100 CTC AAT CGG CAC CTG TCA TCA AGC ATG TTC TGC AAC AAA GAT TGC 0359 Leu Asn Arg His Leu Ser Ser Ser Met Phe Cys Asn Lys Asp Cys 105 110 115 TTG GAG CCG TTA CTT GAT TTT CTT AGA GTG CAC AAA CAT AAA GGA 0404 Leu Glu Pro Leu Leu Asp Phe Leu Arg Val His Lys His Lys Gly 120 125 130 GTT GTC ATG ATG TTG AAT GAT CGG ATA CAG ACT ATC CAG CGT CTT 0449 Val Val Met Met Leu Asn Asp Arg He Gin Thr He Gin Arg Leu 135 140 145 CAG TCT GCA TTG TCT AAA GCT GAG GAT TAT CTT ATC AAA CTT CCA 0494 Gin Ser Ala Leu Ser Lys Ala Glu Asp Tyr Leu He Lys Leu Pro 150 155 160 GCA GAT ACA CCT TAC TCT GAG TTC GAA TTT GTA ATC CAA GGT ATG 0539 Ala Asp Thr Pro Tyr Ser Glu Phe Glu Phe Val He ι_ Gly Met 165 170 175 GGT TTT GAA AGA GGC TGG GGT GAT ACT GCT GAA AGG GTT CTA GAA 05S4 Gly Phe Glu Arg Gly Trp Gly Asp Thr Ala Glu Arg Val Leu Glu 180 185 190 ATG ATG CAT CTA CTA CTA GAT ATC CTT CAG GCT CCC GAT CCG TCT 0629 Met Met His Leu Leu Leu Asp He Leu Gin Ala Pro Asp Pro Ser 195 200 205 ACA TTA GAG ACA TTT CTG GGA AGA CTT CCC ATG GTG TTT AAT GTG 0674 Thr Leu Glu Thr Phe Leu Gly Arg Leu Pro Met Val Phe Asn Val 210 215 220 GTC ATT TTG TCT GTA CAT GGA TAT TTT GGA CAG GCA CAT GTG CTC 0719 Val He Leu Ser Val His Gly Tyr Phe Gly Gin Ala His Val Leu 225 230 235 GGC TTG CCT GAC ACT GGT GGG CAG ATA GTT TAT ATA CTT GAC CAA 0764 Gly Leu Pro Asp Thr Gly Gly Gin He Val Tyr He Leu Asp Gin 240 245 250 GTG CGG TCT CTG GAA CAT GAA ATG CTC CAA CGA ATA AAG AAG CAA 0809 Val Arg Ser Leu Glu His Glu Met Leu Gin Arg He Lys Lys Gin 255 260 265 GGA CTA GAT GTG ACT CCT AGA ATT CTT ATC GTG AGT CGG TTG ATT 0854 Gly Leu Asp Val Thr. Pro Arg He Leu He Val Ser Arg Leu He 270 275 280 CCT GAC GCT AAA GGG ACC ACG TGC AAT CAA CGT ATG GAG AAA GTC 0899 Pro Asp Ala Lys Gly Thr Thr Cys Asn Gin Arg Met Glu Lys Val 285 290 295 AGT GGA ACA GAG CAT GCT AGT ATC CTG AGA GTT CCT TTC CGA TCA 0944 Ser Gly Thr Glu His Ala Ser He Leu Arg Val Pro Phe Arg Ser 300 305 310 GAG AAA GGA ATC CTC CGC AAA TGG ATA TCT AGA TTT GAT GTA TGG 0989 Glu Lys Gly He Leu Arg Lys Trp He Ser Arg Phe Asp Val Trp 315 320 325 CCT TAT TTA GAG ACC TTC ACT GAG GAT GCA GCT GGT GAA ATT ATT 1034 Pro Tyr Leu Glu Thr Phe Thr Glu Asp Ala Ala Gly Glu He He 330 335 340 GGC GAG TTG CAG GGT CGT CCA GAT CTG ATA ATT GGC AAC TAC AGC 1079 Gly Glu Leu Gin Gly Arg Pro Asp Leu He He Gly Asn Tyr Ser 345 350 355 GAT GGG AAT ATA GTT GCT TCT TTA TTG TCC CAC AAA ATG GGT GTC 1124 Asp Gly Asn He Val Ala Ser Leu Leu Ser His Lys Met Gly Val 360 365 370 ACC CAG TGC AAT ATA GCC CAT GCA TTG GAG AAA ACC AAG TAT CCA 1169 Thr Gin Cys Asn He Ala His Ala Leu Glu Lys Thr Lys i r Pro 375 380 385 GAT TCT GAT ATT TAC TGG AAA AGA TTT GAG GAC AAA TAT CAC TTC 1214 Asp Ser Asp He Tyr Trp Lys Arg Phe Glu Asp Lys Tyr His Phe 390 395 400 TCG TGT CAA TTT TCA GCT GAC TTG ATG GCA ATG AAT CAT GCT GAT 1259 Ser Cys Gin Phe Ser Ala Asp Leu Met Ala Met Asn His Ala Asp 405 410 415 TTC ATC ATT ACG AGT ACT TAC CAA GAG ATA GCT GGA ACG AAG AAT 1304 Phe He He Thr Ser Thr Tyr Gin Glu He Ala Gly Thr Lys Asn 420 425 430 ACT GTT GGT CAA TAT GAA AGC CAT AAG GCC TTT ACT TTT CCG GGG 1349 Thr Val Gly Gin Tyr Glu Ser His Lys Ala Phe Thr Phe Pro Gly 435 440 445 CTG TAT CGG GTG GTT CAC GGG ATT GAT GTC TTT GAT CCC AAG TTT 1394 Leu Tyr Arg Val Val His Gly He Asp Val Phe Asp Pro Lys Phe 450 455 460 AAT ATT GTC TCG CCA GGG GCA GAC ATG GCC ATC TAC TTC CCA TTT 1439 Asn He Val Ser Pro Gly Ala Asp Met Ala He Tyr Phe Pro Phe 465 470 475 TCA GAG AAG GAT GTC ACC TGT CTC ACT TCA CTT CAT AGA CTT ATA 1484 Ser Glu Lys Asp Val Thr Cys Leu Thr Ser Leu His Arg Leu He 480 485 490 GAG CAG CTC CTA TTC AAA CCT GAG CAG AAC GAA GAA CAC ATT GGT 1529 Glu Gin Leu Leu Phe Lys Pro Glu Gin Asn Glu Glu His He Gly 495 500 505 GTA TTA GAT GAT ACC TCA AAG CCA ATT ATA TTT TCC ATG GCG AGG 1574 Val Leu Asp Asp Thr Ser Lys Pro He He Phe Ser Met Ala Arg 510 515 520 CTA GAC CGT GTG AAG AAT ATA ACA GGG CTG GTA GAG TGC TAT GGC 1619 Leu Asp Arg Val Lys Asn He Thr Gly Leu Val Glu Cys Tyr Gly 525 530 535 AAG AAT GCG AAA CTC AGG GAA CTG GCA AAC CTG GTT GTA GTG GCT 166 Lys Asn Ala Lys Leu Arg Glu Leu Ala Asn Leu Val Val Val Ala 540 545 550 GGG TAC AAT GAT GTA AAA AAG TCG AAT GAC AGG GAG GAA ATT GCC 170 Gly Tyr Asn Asp Val Lys Lys Ser Asn Asp Arg Glu Glu He Ala 555 560 565 GAA ATC GAG AAG ATG CAC AGG CTT ATA CAG GAG TAT AAT TTA AGA 1754 Glu He Glu Lys Met His Arg Leu He Gin Glu Tyr Asn Leu Arg 570 575 580 GGA CAA TTT CGC TGG ATT GCT TCT CAA ACA AAT AGA GTA CGA AAT 1799 Gly Gin Phe Arg Trp He Ala Ser Gin Thr Asn Arg Val Arg Asn 585 590 595 GGT GAA CTC TAT CGC TAC ATT TGT GAC AAA GGA GGT ATT TTT GCG 1844 Gly Glu Leu Tyr Arg Tyr He Cys Asp Lys Gly Gly He Phe Ala 600 605 610 CAG CCT GCA TTT TAT GAA GCA TTT GGG CTT ACA GTT GTT GAA GCC 1889 Gin Pro Ala Phe Tyr Glu Ala Phe Gly Leu Thr Val Val Glu Ala 615 620 625 ATG ACC TGT GGT CTT CCC ACA TTT GCT ACC TGC CAC GGT GGT CCA 1934 Met Thr Cys Gly Leu Pro Thr Phe Ala Thr Cys His Gly Gly Pro 630 635 640 GCT GAG ATT ATA GAA GAC GGT GTT TCA GGA TTT CAT ATC GAT CCA 1979 Ala Glu He He Glu Asp Gly Val Ser Gly Phe His He Asp Pro 645 650 655 TAT CAT GCT GAT CAG GCA GAA AAA ATG ACT GAA TTC TTT GTC AAG 2024 Tyr His Ala Asp Gin Ala Glu Lys Met Thr Glu Phe Phe Val Lys 660 665 670 TGC AGA GAG GAT CCA AAC TAC TGG ACT AAA ATC TCT GCA GGA GGG 2069 Cys Arg Glu Asp Pro Asn Tyr Trp Thr Lys He Ser Ala Gly Gly 675 680 685 TTA CTA AGG ATC AAA GAA AGA TAT ACC TGG CAA AAG TAT TCT GAA 2114 Leu Leu Arg He Lys Glu Arg Tyr ϊ_ιi Trp Gin Lys Tyr Ser Glu 690 695 700 AGG TTA ATG ACA TTG GCA GGG GTG TAT GGT TTC TGG AAA TAT GTC 2159 Arg Leu Met Thr Leu Ala Gly Val Tyr Gly Phe Trp Lys Tyr Val 705 710 715 TCT AAA CTA GAG AGA AGA GAG ACA CGA CGT TAT CTT GAG ATG TTC 2204 Ser Lys Leu Glu Arg Arg Glu Thr Arg Arg Tyr Leu Glu Met Phe 720 725 . 730 TAC ATT TTG AAG TTC CGT GAT CTG GCC AAC TCT GTT CCG CTG GCA 2249 Tyr He Leu Lys Phe Arg Asp Leu Ala Asn Ser Val Pro Leu Ala 735 740 745 ACA GAT GAA GAG CCT TCT ACT ACT GAT GCA GTT GCG ACA TTC CGT 2294 Thr Asp Glu Glu Pro Ser Thr Thr Asp Ala Val Ala Thr Phe Arg 750 755 760 GGA CCT TGA ACGCTGCTGC TTACTGAGGT TCCAAGTTGT GTATATATTA 2343 Gly Pro End CTGTGAAAGG AATAAGTGTA GCTACACAAA AGGTTCTCAA CTATTAGTAT 2393 CTTCTCTGTG TAAATAACGA GAGTGAAAAA TGTAATATTG TTGATGTCTT 2443 GAAAACTGAG TTTGCTTTGT TTATTTTTAA GTGTATGACA ATATGTATCA 2493 TATAACGGAT TCTTCAGTGA TCATATCAAA AACTACTGAC CATCGAAGTT 2543 AATGAAAATC GACAGCAACA 2563 5 These sequences can also be combined together in a suitable plasmid which leads to a combination of the individual characteristics, conditioned by the expression of the protein. 10 The promoter should ensure that the foreign gene is expressed in the plant. The promoter can be so chosen that the expression occurs only in specified tissues, at a determined time point in the plant's development or at a time point determined by outside influences. The promoter 15 can be homologous or heterologous to the plant. Suitable promoters are e.g. the promoter of the 35S RNA of the cauliflower mosaic virus, the patatin promoter B33 (Rocha-Sosa et al. (1989) EMBO J 8: 23-29) or a promoter that ensures an expression only in photosynthetically active tissues. Other promoters can be used which ensure an expression only in specified organs, such as the root, tuber, seed, stem or specified cell types such as mesophyllic, epidermal or transport cells. The coding sequences described herein contain the information for the formation of an RNA for the large subunit of the ADP-glucose-pyrophosphorylase and the sucrose-phosphate-synthase (SPS) and a part of the information for formation of the small subunit of the ADP-glucose-pyrophosphorylase as well as the sucrose-synthase, that are suitable for the formation of anti-sense RNA to the corresponding genes. Whether a translatable mRNA or an anti-sense nucleic acid is formed, depends on the orientation of the coding sequence in relation to the promoter. If the 3' end of the coding sequence is fused to the 3' end of the promoter, an anti-sense RNA results, and by fusion of the 5' end of the coding to the 3' end of the promoter a translatable RNA results. This latter leads to an increase of the enzyme activity in the cell, whilst the first leads to a reduction of the enzyme activity in the cell. The coding sequence for the large and small subunit of the ADP-glucose-pyrophosphorylase, the sucrose phosphate synthase and the sucrose synthase can be one of those described in this invention or can be one that is derived by modifications of the sequences described above. Thereby especially modifications of the sequences can be considered which lead to by-passing of the plant's own regulation mechanisms. Modifications to the DNA sequences of the invention can be by known methods, such as e.g. base exchange or targeted or non-targeted mutagenesis. The so-formed derivatives of the DNA sequences of the invention are also within the scope of the invention. With plasmids, which contain one or more of the DNA sequences of the invention, sugar beet can be transformed with the object of raising and/or reducing the enzyme activity and/or the change of the sucrose concentration. For the introduction of the DNA sequences of the invention in sugar beet, a large number of cloning vectors are available, which contain a replication signal for E . coli and a marker, which allows a selection of the transformed cells. According to the introduction method of the desired gene in the plant, other DNA sequences may be suitable. Should the Ti- or Ri-plasmid be used, e.g. for the transformation of the plant cell, then at least the right boundary, often however both the right and left boundary of the Ti- and Ri-Plasmiα T-DNA, is attached, as a flanking region, to the gene being introduced. The use of T-DNA for the transformation of plants cells has been intensively researched and is well described in EP 120 516; Hoekama, In: The Binary Plant Vector System, Offset-drukkerij Kanters B.V. Alblasserda , (1985) , Chapter V; Fraley, et al., Crit. Rev. Plant Sci., 4:1-46 and An et al. (1985) EMBO J. 4: 277-287. Once the introduced DNA is integrated in the genome, it is as a rule stable there and remains also in the offspring of the original transformed cells. It normally contains a selection marker, which induces resistance in the transformed plant cells against a biocide or antibiotic such as kanamycin, G 418, bleomycin, hygro ycin or phosphinotricin etc. The individual marker employed should therefore allow the selection of transformed cells from cells, which lack the introduced DNA. For the introduction of DNA into a plant, besides transformation using Agrobacteria, there are many other techniques available. These techniques include the fusion of protoplasts, microinjection of DNA and electroporation, as well as ballistic methods and virus infection. From the transformed plant material, whole plants can be regenerated in a suitable medium, which contains antibiotics or biocides for the selection. The resulting plants can then be tested for the presence of introduced DNA. No special demands are placed on the plasmids in injection and electroporation. Simple plasmids, such as e.g. pUC-derivatives can be used. Should however whole plants be regenerated from such transformed cells the presence of a selectable marker gene is necessary. The transformed cells grow within the plants in the usual manner (see also McCormick et al. (1986) Plant Cell Reports 5: 81-84) . These plants can be grown normally and crossed with plants, that possess the same transformed genes or different. The resulting hybrid individuals have the corresponding phenotypical properties. In order to understand the examples forming the basis of this invention all the processes necessary for these tests and which are known per se will first of all be listed: 1. Cloning process The vectors pUC 18/19 and Ml3mplθ series (Yanisch-Perron et al. (1985) Gene 33: 103-119), as well as the vector EMBL 3 (Frischauf et al. (1983) J Mol Biol 170: 827- 842) were used for cloning. For the plant transformations, the gene constructs were cloned in the binary vector BIN 19 (Bevan (1984) Nucl. Acids Res 12: 8711-8720) Bacterial strains The E . coli strain BMH71-18 (Messing et a_l. , Proc. Natl. Acad. Sci. USA (1977), 24, 6342-6346) or TBl was used for the pUC and M13 mP vectors. For the vector BIN19, the E . coli strain TBl exclusively, was used. TBl is a recombiήant-negative, tetracycline- resistant derivative of strain JM101 (Yanisch-Perron et al. , Gene (1985) , 33, 103-119) . The genotype of the TBl strain is (Bart Barrel, personal communication) : F'(traD36, proAB, lad, lacZΔM15) , Δ(lac, pro) , SupE, this, recA, Sri: :TnlO (TcR) . The transformation of the plasmids into the potato plants was carried out using Agrobacterium tumefaciens strain LBA4404 (Bevan, (1984), Nucl. Acids Res. .12., 8711-8720) . 3. Transformation of Agrobacterium tumefaciens In the case of BIN19 derivatives, the insertion of the DNA into the Agrobacterium was effected by direct transformation in accordance with the method of Holsters et al., (1978) (Mol Gene Genet 163: 181-187) . The plasmid DNA of the transformed Agrobacterium was isolated in accordance with the method of Birnboim and Doly (1979) (Nucl Acids Res 7: 1513-1523) and was analysed by gel electrophoresis after suitable restriction cleavage. 4. Sucrose phosphate-synthase activity test The sucrose phosphate-synthase activity was determined according to the method of Siegel and Stitt (1990, Plant Science 66: 205-210) in a two stage analysis. To 180 μl of a solution of 50mM HEPES/KOH (pH 7.4) , 5mM magnesium chloride, 5mM fructose-6-phosphate, 25mM glucose-6-phosphate and 6mM uridine-5'-diphosphoglucose, 20 μl of probe was added and incubated for 10 minutes at 25°C. It was heated for 3 minutes at 95°C, to complete the reaction. After centrifuging, the supernatant was spectroscopically analysed for the liberation of uridine-5'-diphosphate, whereby a pyruvate-kinase coupling enzyme reaction was used. Preparations without hexose phosphate, as well as the measurement of the recovery of added uridine-5'-diphosphate act as controls. Examples Example 1 Cloning of cDNA to large and small subunits of the ADP glucose pyrophosphorylase of sugar beet. From the storage roots of 3-4 month old sugar beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Bioche 163, 16-20) . Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an Xhol recognising position and for synthesis of the first cDNA strand methylated cytidine nucleotide was inserted. After synthesis of the two strands an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease Xhol. In this way the hemimethylation of cDNA was hindered so that an internal Xhol recognition position is cut. By these procedures there is obtained a population of cDNA molecules, that can be cloned directed into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of reco binant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with the total cDNA fragment of the large and/or subunit of the AGPase of potato (Mϋller-Roeber et al., 1990, MGG 224, 136-146) as an EcoRI-fragment. The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions individual clones were analysed by determination of the primary sequence. Example 2 Cloning of cDNA to sucrose-phosphate-synthase (SPS) from sugar beet. From the storage roots of 3-4 month old suga. beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Bioche 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 2b, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an Xhol recognising position and for synthesis of the first cDNA strand methylated cytidine nucleotide was inserted. After synthesis of the two strands an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease Xhol. In this way the hemi ethylation of cDNA was hindered so that an internal Xhol recognition position is cut. By these procedures there is obtained a population of cDNA molecules, that can be cloned directed into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with the total cDNA fragment of the sucrose-phosphate-synthase (SPS) from spinach (Sonnewald, 1992, Planta) as Notl. The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions individual clones were analysed by determination of the primary sequence. Example 3 Cloning of cDNA to sucrose-synthase from sugar beet. From the storage roots of 3-4 month old sugar beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Bioche 163, 16-20) . Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an Xhol recognising position and for synthesis of the first cDNA strand methylated cytidine nucleotide was inserted. After synthesis of the two strands an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease Xhol. In this way the hemi ethylation of cDNA was hindered so that an internal Xhol recognition position is cut. By these procedures there is obtained a population of cDNA molecules, that can be cloned directed into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then parallel is probed with both EcoRI/BgHI sub fragments sucrose synthase from maize (Worrell et al., 1991, Plant Cell 3, 1121-1130) . The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions individual clones were analysed by determination of the primary sequence. Example 4 Determination of the nucleotide sequence of the ADP glucose pyrophosphorylase, the sucrose synthase and the sucrose phosphate synthase of sugar beet and derivation of the corresponding amino acid sequences The nucleotide sequences of the insertions obtained from Examples 1 -3 , were determined by standard methods by means of the dideoxy method (Sanger et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467) . The nucleotide sequences and the amino acid sequences derived therefrom are given in the sequence protocols Seq. ID No. 1-4. The sequences are shown earlier; the protocols are as follows: SEQ ID NO: 1 SEQUENCE TYPE: Nucleotide with corresponding protein SEQUENCE LENGTH: 1924 base pairs STRANDEDNESS: single TOPOLOGY: linear MOLECULE TYPE: CDNA ORIGINAL SOURCE ORGANISM: Beta vulgaris IMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap FEATURES: from 206 to 1770 coding region PROPERTIES: ADP-glucose-pyrophosphorylase, large subunit SEQ ID NO: 2 SEQUENCE TYPE: Nucleotide with corresponding protein SEQUENCE LENGTH: 1763 base pairs STRANDEDNESS: single TOPOLOGY: linear MOLECULE TYPE: cDNA ORIGINAL SOURCE ORGANISM: Beta vulgaris IMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap FEATURES: from 3 to 1469 coding region PROPERTIES: ADP-glucose-pyrophosphorylase, small subunit SEQ ID NO: 3 SEQUENCE TYPE: Nucleotide with corresponding protein SEQUENCE LENGTH: 3635 base pairs STRANDEDNESS: single TOPOLOGY: linear MOLECULE TYPE: cDNA ORIGINAL SOURCE ORGANISM: Beta vulgaris IMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap FEATURES: from 31 to 3164 coding region PROPERTIES: Sucrose-phosphate-synthase SEQ ID NO: 4 SEQUENCE TYPE: Nucleotide with corresponding protein SEQUENCE LENGTH: 2563 base pairs STRANDEDNESS: single TOPOLOGY: linear MOLECULE TYPE: cDNA ORIGINAL SOURCE ORGANISM: Beta vulgaris IMMEDIATE EXPERIMENTAL SOURCE: cDNA library in Phage Lamda zap FEATURES: from 3 to 2300 coding region PROPERTIES: Sucrose synthase

Claims: CLAIMS 1. DNA sequence with the coding region for die large subunit of ADP-glucose-pyrophosphorylase for the 5 preparation of sugar beet with changed sucrose concentration, characterised in that the sequence has the following nucleotide sequence (Seq. ID No.l) : CAAAAGAAAA ACTTCCCATT TCTACTTCTT TGCACAATAT AATTTCCCAC 0050 CAATTTTTCT TTAAATTTCT CACTTTCATT TAATCAGTTT TCAGCAACAT 0100 TCTGATACTC GACAACCCAC TTTCTGTTCT CCCAAGATTC CAAACCTCTG 0150 ATTCTCATTC CACTAATATT TTTGCTTATT TTTTTTCTGG ATTTAAAGAA 0200 AAGCT ATG GAT GCA AGT GCA GCA GCC ATA AAT GTC AAT GCC CAT 0243 Met Asp Ala Ser Ala Ala Ala He Asn Val Asn Ala His 5 10 TTA ACA GAA GTT GGA AAG AAA CGT TTT TTA GGA GAG AGA ATC AGT 0288 Leu Thr Glu Val Gly Lys Lys Arg Phe Leu Gly Glu Arg He Ser 15 20 25 CAA AGT TTG AAG GGT AAA GAT CTG AGA GCT CTG TTT TCA AGA ACT 0333 Gin Ser Leu Lys Gly Lys Asp Leu Arg Ala Leu Phe Ser Arg Thr 30 35 40 GAG AGC AAG GGT AGA AAT GTC AAT AAA CCT GGG GTT GCA TTT TCT 0378 Glu Ser Lys Gly Arg Asn ' Val Asn Lys Pro Gly Val Ala Phe Ser 45 50 55 GTT CTC ACC TCA GAT TTT AAT CAA AGT GTT AAA GAA TCT TTG AAA 0423 Val Leu Thr Ser Asp Phe Asn Gin Ser Val Lys Glu Ser Leu Lys 60 65 70 TAT GAG CCA GCA TTA TTT GAA TCT CCA AAA GCT GAC CCA AAA AAT 0468 Tyr Glu Pro Ala Leu Phe Glu Ser Pro Lys Ala Asp Pro Lys Asn 75 80 85 GTG GCT GCA ATT GTG CTG GGT GGT GGT GCT GGG ACT CGC CTC TTT 0513 Val Ala Ala He Val Leu Gly Gly Gly Ala Gly Thr Arg Leu Phe 90 95 100 CCT CTT ACT AGC AGG AGA GCT AAG CCA GCA GTG CCA ATT GGA GGG 0558 Pro Leu Thr Ser Arg Arg Ala Lys Pro Ala Val Pro He Gly Gly 105 110 115 TGT TAC AGG CTG ATT GAT GTG CCT ATG AGC AAC TGC ATC AAC AGT 0603 Cys Tyr Arg Leu He Asp Val Pro Met Ser Asn Cys He Asn Ser 120 125 130 GGC ATT AGA AAG ATT TTC ATT CTT ACC CAG TTC AAT TCG TTT TCG 0648 Gly He Arg Lys He Phe He Leu Thr Gin Phe Asn Ser Phe Ser 135 140 145 CTT AAT CGT CAT CTT GCT CGA ACC TAT AAT TTT GGA GAT GGT GTG 0693 Leu Asn Arg His Leu Ala Arg Thr Tyr Asn Phe Gly Asp Gly Val 150 155 160 AAT TTT GGG GAT GGC TTT GTG GAG GTT TTT GCT GCT ACA CAA ACA 0738 Asn Phe Gly Asp Gly Phe Val Glu Val Phe Ala Ala Thr Gin Thr 165 170 175 CCT GGA GAA TCA GGA AAG AAA TGG TTC CAG GGC ACC GCT GAT GCA 0783 Pro Gly Glu Ser Gly Lys Lys Trp Phe Gin Gly Thr Ala Asp Ala 180 185 190 GTA AGA CAG TTT TTC TGG GCA TTT GAG GAT TCC AAA TCC AAG GAT 0828 Val Arg Gin Phe Phe Trp Ala Phe Glu Asp Ser Lys Ser Lys Asp 195 200 205 GTC GAG CAT ATA GTT ATT TTA TCC GGT GAT CAT CTT TAC CGA ATG 0873 Val Glu His He Val He Leu Ser Gly Asp His Leu Tyr Arg Met 210 215 220 GAT TAC ATG AGT TTT TGG CAG AAG CAC ATT GAC ACC AAT GCT GAT 0918 Asp Tyr Met Ser Phe Trp Gin Lys His He Asp Thr Asn Ala Asp 225 230 235 ATT ACA GTG TCA TGC ATA CCC ATG GAT GAC AGC CGT GCA TCG GAT 0963 He Thr Val Ser Cys He Pro Met Asp Asp Ser Arg Ala Ser Asp 240 245 250 TAT GGG CTG ATG AAG ATT GAT CAC ACT GGA CGC ATT GTC CAT TTT 1008 Tyr Gly Leu Met Lys He Asp His Thr Gly Arg He Val His Phe 255 260 265 GCA GAA AAA CCC AAG GGT TCT GAT CTA ACA GCA ATG CAA GTA GAT 1053 Ala Glu Lys Pro Lys Gly Ser Asp Leu Thr Ala Met Gin Val Asp 270 275 280 ACA ACT GTT CTT GGG CTC TCT GAC CTT GAA GCT ATG TCA AAT CCA 1098 Thr Thr Val Leu Gly Leu Ser Asp Leu Glu Ala Met Ser Asn Pro 285 290 295 TAT ATT GCA TCA ATG GGT GTT TAT GTC TTT CGA ACG GAT GTT CTT 1143 Tyr He Ala Ser Met Gly Val Tyr Val Phe Arg Thr Asp Val Leu 300 305 310 ATG GAG CTT CTC AAT CGA AAA TAC CCT TCA AGC AAT GAT TTT GGC 1188 Met Glu Leu Leu Asn Arg Lys Tyr Pro Ser Ser Asn Asp Phe Gly 315 320 325 TCT GAG ATT ATT CCT TCA GCT GTA GGA GAG TCT AAT GTT CAG GCA 1233 Ser Glu He He Pro Ser Ala Val Gly Glu Ser Asn Val Gin Ala 330 335 340 TAT CTA TTT AAT GAC TAC TGG GAG GAT ATC GGA ACC ATA ' AAG TCT 1278 Tyr Leu Phe Asn Asp Tyr Trp Glu Asp He Gly Thr He Lys Ser 345 350 355 TTC TTT GAT TCC AAT TTG GCC CTT ACA CAA CAG CCT CCC AAG TTT 1323 Phe Phe Asp Ser Asn Leu Ala Leu Thr Gin Gin Pro Pro Lys Phe 360 365 370 GAA TTC TAC GAT CCA AAA ACA CCT TTT TAT ACA TCT GCA AGA TTT 1368 Glu Phe Tyr Asp Pro Lys Thr Pro Phe Tyr Thr Ser Ala Arg Phe 375 380 385 CTG CCT CCT ACA AAA GTC GAC AGG TGC AAG ATT GTC GAT TCC ATT 1413 Leu Pro Pro Thr Lys Val Asp Arg Cys Lys He Val Asp Ser He 390 395 400 GTA TCC CAT GGT TGT TTT CTA CAG GAG TCT AGC ATC CAA CAT TCC 1458 Val Ser His Gly Cys Phe Leu Gin Glu Ser Ser He Gin His Ser 405 410 415 ATT GTT GGT GTT CGC TCA AGA TTA GAG TCC GGG GTT GAG TTC CAG 1503 He Val Gly Val Arg Ser Arg Leu Glu Ser Gly Val Glu Phe Gin 420 425 430 GAC ACC ATG ATG ATG GGC GCA GAT TAC TAT CAA ACT GAA TCA GAA 1548 Asp Thr Met Met Met Gly Ala Asp Tyr Tyr Gin Thr Glu Ser Glu 435 440 445 ATT GCT TCT CTG CTT GCT ' GAG GGA AAG ' GTT CCT GTT GGT GTC GGA 1593 He Ala Ser Leu Leu Ala Glu Gly Lys Val Pro Val Gly Val Gly 450 455 460 CAG AAT ACC AAA ATA AAG AAT TGC ATA ATT GAC AAG AAC GCC AAA 1638 Gin Asn Thr Lys He Lys Asn Cys He He Asp Lys Asn Ala Lys 465 470 475 ATT GGA AAA GAT GTG GTA ATC GCA AAC ACG GAT GGT GTT GAG GAA 1683 He Gly Lys Asp Val Val He Ala Asn Thr Asp Gly Val Glu Glu 480 485 490 GCA GAT AGA CCA AAT GAA GGC TTT TAC ATC AGG TCG GGC ATT ACC 1728 Ala Asp Arg Pro Asn Glu Gly Phe Tyr He Arg Ser Gly He Thr 495 500 505 ATC ATT TTG AAG AAC GCA ACC ATA CAA GAC GGT CTT GTG ATT TAG 1773 He He Leu Lys Asn Ala Thr He Gin Asp Gly Leu Val He End 510 515 520 ATTTAATCAT AACCTCATTA GAAAGAAATA ATTTTGCATG ATTTCCTTTT 1823 CATGTAACCT AAACTGGCTA AACCACGAGG TTTTCTCATC TGTATATATA 1873 ATATGTCTAT AACTATGGAT AATCTTAATA AAAAAAAAAA AAAAAAAAAA 1923 1924 2. DNA sequence with the coding region for the small subunit of ADP-glucose-pyrophosphalase for the preparation of sugar beet with changed sucrose concentration, characterised in that the sequence has the following nucleotide sequence (Seq. ID No.2) GG ATA ACT GTG CCA TCA ACC TCC TCA AAG AAC CTC CAA AAT AGC 0044 He Thr Val Pro Ser Thr Ser Ser Lys Asn Leu Gin Asn Ser 5 10 CTC GCA TTC TCC TCT TCT TCT CTC TCC GGC GAC AAA ATT CAA ACG 0089 Leu Ala Phe Ser Ser Ser Ser Leu Ser Gly Asp Lys He Gin Thr 15 20 25 ACG TCA TTT CTC AAC CGC CGA TAT TGT AGA ATC TCT TCT AGA GCT 01 4 Thr Ser Phe Leu Asn Arg Arg Tyr Cys Arg He Ser Ser Arg Ala 30 35 40 CCG ATT GTT GTC TCT CCC AAA GCT GTT TCT GAT TCT AAG AAT TCG 017 Pro He Val Val Ser Pro Lys Ala Val Ser Asp Ser Lys Asn Ser 45 50 55 CAG ACT TGT CTT GAC CCT GAA GCC AGC CGT AGT GTT CTT GGT ATT 0224 Gin Thr Cys Leu Asp Pro Glu Ala Ser Arg Ser Val Leu Gly He 60 65 70 ATA CTT GGA GGT GGT GCT GGT ACA CGT CTT TAC CCG TTG ACT AAG 0269 He Leu Gly Gly Gly Ala Gly Thr Arg Leu Tyr Pro Leu Thr Lys 75 80 85 AAG AGA GCC AAA CCA GCC GTG CCA CTC GGT GCT AAT TAT AGG CTT 0314 Lys Arg Ala Lys Pro Ala Val Pro Leu Gly Ala Asn Tyr Arg Leu 90 95 100 ATT GAT ATC CCA GTG AGC AAT TGT TTG AAC AGT AAT ATT TCC AAA 0359 He Asp He Pro Val Ser Asn Cys Leu Asn Ser Asn He Ser Lys 105 110 110 ATA TAT GTT CTT ACA CAA TTC AAT TCT GCT TCT CTG AAT CGT CAT 0404 He Tyr Val Leu Thr Gin Phe Asn Ser Ala Ser Leu Asn Arg His 115 120 125 CTT TCG CGG GCA TAT GCT AGC AAC ATG GGA GGA TAC AAA AAT GAG 0449 Leu Ser Arg Ala Tyr Ala Ser Asn Met Gly Gly Tyr Lys Asn Glu 130 135 140 GGG TTT GTA GAA GTT CTT GCT GCT CAG CAA AGT CCA GAG AT CCA 0494 Gly Phe Val Glu Val Leu Ala Ala Gin Gin Ser Pro Glu Asn Pro 145 150 155 AAC TGG TTT CAG GGT ACA GCT GAT GCT GTT AGG CAA TAT CTG TGG 0539 Asn Trp Phe Gin Gly Thr Ala Asp Ala Val Arg Gin Tyr Leu Trp 160 165 170 CTT TTC GAA GAG CAC AAT GTT CTT GAG TAC TTG ATT CTT GCT GGT 0584 Leu Phe Glu Glu His Asn Val Leu Glu Tyr Leu He Leu Ala Gly 175 180 185 GAC CAT TTG TAT CGA ATG GAT TAT GAA AGA TTT GTC CAA GCT CAC 0629 Asp His Leu Tyr Arg Met Asp Tyr Glu Arg Phe Val Gin Ala His 190 195 200 AGA GAA ACT GAT GCA GAC ATT ACT GTT GCT GCA TTG CCA ATG GAT 0674 Arg Glu Thr Asp Ala Asp He Thr Val Ala Ala Leu Pro Met Asp 205 210 215 GAA AAG CGT GCT ACT GCA TTT GGT TTG ATG AAA ATT GAT GAA GAA 0719 Glu Lys Arg Ala Thr Ala Phe Gly Leu Met Lys He Asp Glu Glu 220 225 230 GGA AGA ATT ATT GAG TTT GCC GAG AAA CCG AAA GGA GAA CAA TTG 0764 Gly Arg He He Glu Phe Ala Glu Lys Pro Lys Gly Glu Gin Leu 235 240 245 AAA GCT ATG AAG GTT GAT ACC ACA ATC CTG GGT CTG GAC GAT GAG 0809 Lys Ala Met Lys Val Asp Thr Thr He Leu Gly Leu Asp Asp Glu 250 255 260 AGA GCA AAA GAA ATG CCA TTC ATA GCC AGC ATG GGC ATA TAT GTT 0854 Arg Ala Lys Glu Met Pro Phe He Ala Ser Met Gly He Tyr Val 265 270 275 ATT AGC AAA GAT GTA ATG CTT AAT CTG CTT CGG GAG CAA TTT CCT 0899 He Ser Lys Asp Val Met Leu Asn Leu Leu Arg Glu Gin Phe Pro 280 285 290 GGT GCT AAT GAT TTT GGA AGT GAA GTT ATT CCA GGC GCC ACT TCC 0944 Gly Ala Asn Asp Phe Gly Ser Glu Val He Pro Gly Ala Thr Ser 295 300 305 ATA GGG TTG AGA GTC CAA GCT TAT TTG TAT GAT GGT TAC TGG GAG 0989 He Gly Leu Arg Val Gin Ala Tyr L_u Tyr Asp Gly Tyr Trp Glu 310 315 320 GAT ATT GGT ACC ATT GAA GCT TTT TAC AAT GCT AAC TTG GGA ATC 1034 Asp He Gly Thr He Glu Ala Phe Tyr Asn Ala Asn Leu Gly He 325 330 335 ACC AAA AAG CCG GTG CCA GAT TTT AGC TTC TAT GAT CGT TCA TCT 1079 Thr Lys Lys Pro Val Pro Asp Phe Ser Phe Tyr Asp Arg Ser Ser 340 345 350 CCA ATT TAT ACA CAA CCT CGG TAT TTG CCT CCT TCA AAG ATG CTT 1124 Pro He Tyr Thr Gin Pro Arg Tyr Leu Pro Pro Ser Lys Met Leu 355 360 365 GAT GCT GAT ATA ACT GAC AGC GTC ATC GGT GAA GGC TGT GTT ATT 1169 Asp Ala Asp He Thr Asp Ser Val He Gly Glu Gly Cys Val He 370 375 380 AAG AAC TGT AAG ATT CAT CAT TCT GTT ATC GGA CTT CGA TCT TGT 1214 Lys Asn Cys Lys He His His Ser Val He Gly Leu Arg Ser Cys 385 390 395 ATC TCG GAG GGT GCA ATC ATT GAG GAC ACA CTG TTG ATG GGA GCT 1259 He Ser Glu Gly Ala He He Glu Asp Thr Leu Leu Met Gly Ala 400 405 410 GAT TAT TAT GAG ACT GAT GCT GAT CGG AAA TTC CTG GCT GCT AAG 1304 Asp Tyr Tyr Glu Thr Asp Ala Asp Arg Lys Phe Leu Ala Ala Lys 415 420 425 GGT AGT GTA CCT ATT GGA ATT GGG AAT GCA CGT ATT GGG GAT GAT 1349 Gly Ser Val Pro He Gly He Gly Asn Ala Arg He Gly Asp Asp 430 435 440 GTC AAG ATT ATC AAC AGT GAC AAT GTA CAA GAA GCA GCA AGA GAA 1394 Val Lys He He Asn Ser Asp Asn Val Gin Glu Ala Ala Arg Glu 445 450 455 ACA GAC GGA TAC TTC ATA AAG AGC GGA ATA GTC ACT ATA ATC AAG 143 Thr Asp Gly Tyr Phe He Lys Ser Gly He Val Thr He He Lys 460 465 470 GAC GCC ATG ATT CCA AGT GGA ACT GTA ATC TAG AAATGGAGCA 1482 Asp Ala Met He Pro Ser Gly Thr Val He End 475 480 485 TATAATAAAT ATCACTGCCT ATTTACAGTA- CCTATCTGAG TCTCCCACCA 1532 TGACCCTTTG ATTCAATCTT TTAGTTATGT AAATATTTTT GGCTTTTGCG 1582 ATTTTGCCAT AAATTTGAAG AAGCGAGGAT TCAGGGACGA TAGTGCTATG 1632 AATTGGAAGA AAGGATTTGG GGGATATCTT TGTAAAGACA TTTTGACTAC 1682 TGGGCACTAA AAATTTGGTA ATGCTATACC AAAATATATA AAAAGATCTT 1732 GCTGGGTTTT GGTAAAAAAA AAAAAAAAAA A 1763 3. DNA sequence with the coding region for sucrose phosphate - synthase for the preparation of sugar beet with changed sucrose concentration, characterised in that the sequence has the following 5 nucleotide sequence (Seq. ID No.3) : GGGCTGCAGG GAAGCTCTGA ACTTCAAAA ATG GCG GGA AAT GAT 0044 Met Ala Gly Asn Asp 5 TGG ATA AAC AGT TAT TTA GAG GCA ATT CTG GAT GTG GGT CCA GGA 0089 Trp He Asn Ser Tyr Leu Glu Ala He Leu Asp Val Gly Pro Gly 10 15 20 CTT GAT GAT GCA AAA TCA TCT TTG CTT TTG AGA GAA AGA GGC AGG 0134 Leu Asp Asp Ala Lys Ser Ser Leu Leu Leu Arg Glu Arg Gly Arg 25 30 35 TTT AGT CCT ACT CGT TAC TTT GTT GAA GAA GTT ATC ACT GGT TTT 0179 Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu Val He Thr Gly Phe 40 45 50 GAT GAA ACC GAC CTT CAT CGT TCA TGG GTT CGG GCA CAA GCA ACA 0224 Asp Glu Thr Asp Leu His Arg Ser Trp Val Arg Ala Gin Ala Thr 55 60 65 AGG AGT CCT CAA GAG AGG AAT ACT AGA TTG GAG AAC ATG TGT TGG 0269 Arg Ser Pro Gin Glu Arg Asn Thr Arg Leu Glu Asn Met Cys Trp 70 75 80 AGA ATT TGG AAT TTG GCT CGT CAG AAG AAG CAG CTT GAG AAT GAA 0314 Arg He Trp Asn Leu Ala Arg Gin Lys Lys Gin Leu Glu Asn Glu 85 90 95 GAA GCT CAG CGG AAG ACA AAA CGT CGT ATG GAG CTT GAG AGG GGT 0359 Glu Ala Gin Arg Lys Thr Lys Arg Arg Met Glu Leu Glu Arg Gly 100 105 110 CGT CGA GAA GCA ACT GCT GAT ATG TCG GAG GAC TTA TCA GAA GGC 0404 Arg Arg Glu Ala Thr Ala Asp Met Ser Glu Asp Leu Ser Glu Gly 115 120 125 GAA AAG GAC ATT TCA GCT CAT GGT GAT AGC ACC CGT CCT AGA TTG 0449 Glu Lys Asp He Ser Ala His Gly Asp Ser Thr Arg Pro Arg Leu 130 135 140 CCA AGA ATA AAT TCT CTT GAT GCT ATG GAG ACA TGG ATT AGT CAA 0494 Pro Arg He Asn Ser Leu Asp Ala Met Glu Thr Trp He Ser Gin 145 150 155 CAA AAG GAA AAA AAA CTC TAC CTT GTT TTG ATA AGT CTT CAT GGT 0539 Gin Lys Glu Lys Lys Leu Tyr Leu Val Leu He Ser Leu His Gly 160 165 170 TTG ATA CGA GGT GAA AAC ATG GAA CTT GGC CGT GAT TCT GAT ACT 0584 Leu He Arg Gly Glu Asn Met Glu Leu Gly Arg Asp Ser Asp Thr 175 180 185 GGT GGT CAG GTT AAG TAT GTG GTT GAG CTT GCA AGG GCT CTA GGT 0629 Gly Gly Gin Val Lys Tyr Val Val Glu Leu Ala Arg Ala Leu Gly 190 195 200 TCG ATG CCA GGT GTT TAT AGA GTT GAT TTG CTA ACT AGG CAA GTT 0674 Ser Met Pro Gly Val Tyr Arg Val Asp Leu Leu Thr Arg Gin Val 205 210 215 TCA TCT CCT GAC GTG GAT TGG AGT TAT GGG GAG CCT ACT GAG ATG 0719 Ser Ser Pro Asp Val Asp Trp Ser Tyr Gly Glu Pro Thr Glu Met 220 225 230 CTG AAT CCA AGG GAT TCC AAT GGT TTT GAT GAT GAT GAT GAT GAA 0764 Leu Asn Pro Arg Asp Ser Asn Gly Phe Asp Asp Asp Asp Asp Glu 235 240 245 ATG GGA GAG AGT AGT GGT GCT TAC ATT GTT CGT ATA CCA TTT GGG 0809 Met Gly Glu Ser Ser Gly Ala Tyr He Val Arg He Pro Phe Gly 250 255 260 CCG AGG GAT AAG TAT ATC GCA AAA GAA GAG CTT TGG CCC TAT ATT 0854 Pro Arg Asp Lys Tyr He Ala Lys Glu Glu Leu Trp Pro Tyr He 265 270 275 CCT GAA TTT GTT GAT GGT GCT CTA AAC CAC ATA GTT CAA ATG TCC 0899 Pro Glu Phe Val Asp Gly Ala Leu Asn His He Val Gin Met Ser 280 285 290 AAA GTT TTA GGT GAG CAA ATT GGT AGC GGG GAA ACA GTT TGG CCA 0944 Lys Val Leu Gly Glu Gin He Gly Ser Gly Glu Thr Val Trp Pro 295 300 305 GTT GCC ATT CAT GGA CAT TAT GCT GAT GCT GGT GAT TCT GCT GCT 0989 Val Ala He His Gly His Tyr Ala Asp Ala Gly Asp Ser Ala Ala 310 315 320 CTT CTT TCT GGT GGC CTA AAT GTT CCA ATG CTT TTA ACG GGG CAT 1034 Leu Leu Ser Gly Gly Leu Asn Val Pro Met Leu Leu Thr Gly His 325 330 335 TCT CTT GGC CGA GAC AAG TTA GAG CAG CTC CTC AAA CAG GGT CGA 1079 Ser Leu Gly Arg Asp Lys Leu Glu Gin Leu Leu Lys Gin Gly Arg 340 345 350 ATG TCT AAA GAT GAC ATA AAC AAT ACA TAC AAA ATA ATG CGT AGG 1124 Met Ser Lys Asp Asp He Asn Asn Thr Tyr Lys He Met Arg Arg 355 360 365 ATA GAA GCC GAA GAG TTA TCA CTT GAT GCC TCT GAG ATA GTC ATA 1169 He Glu Ala Glu Glu Leu Ser Leu Asp Ala Ser Glu He Val He 370 375 380 ACT AGT ACA AGA CAA GAA ATA GAA GAG CAA TGG CAC CTC TAT GAT 1214 Thr Ser Thr Arg Gin Glu He Glu Glu Gin Trp His Leu Tyr Asp 335 390 395 GGG TTT GAT CCT GTG CTA GAA CGT AAA CTC CGT GCT AGG ATG AAG 1259 Gly Phe Asp Pro Val Leu Glu Arg Lys Leu Arg Ala Arg Met Lys 400 405 410 CGT GGT GTA AGC TGT TAT GGA AGG TTC ATG CCC CGG ATG GTT GTT 1304 Arg Gly Val Ser Cys Tyr Gly Arg Pne Met Pro Arg Met Val Val 415 420 425 ATT CCT CCT GGA ATG GAA TTC AAT CAT ATT GTT CCA CAT GAG GGT 1349 He Pro Pro Gly Met Glu Phe Asn His He Val Pro His Glu Gly 430 435 440 GAT ATG GAT GGT GAA ACA GAA GAA ACT GAA GAG CAT CCT ACA TCA 1394 Asp Met Asp Gly Glu Thr Glu Glu Thr Glu Glu His Pro Thr Ser 445 450 455 CCT GAT CCA CCT ATC TGG GCT GAG ATT ATG CGC TTC TTT TCT AAA 1439 Pro Asp Pro Pro He Trp Ala Glu He Met Arg Phe Phe Ser Lys 460 465 470 CCA AGG AAG CCA ATG ATA CTT GCC CTT GCT AGG CCT GAC CCG AAG 1484 Pro Arg Lys Pro Met He Leu Ala Leu Ala Arg Pro Asp Pro Lys 475 480 485 AAG AAT ATC ACG ACT TTG GTC AAA GCA TTT GGA GAA TGC CGT CCA 1529 Lys Asn He Thr Thr Leu Val Lys Ala Phe Gly Glu Cys Arg Pro 490 495 500 CTA AGG GAG CTA GCT AAT CTT ACT CTT ATA ATG GGT AAC CGA GAT 1574 Leu Arg Glu Leu Ala Asn Leu Thr Leu He Met Gly Asn Arg Asp 505 510 515 GGT ATT GAC GAG ATG TCA AGC ACC AGT TCT TCA GTT CTC CTG TCA 1619 Gly He Asp Glu Met Ser Ser Thr Ser Ser Ser Val Leu Leu Ser 520 525 530 GTG CTT AAG CTA ATT GAT CAA TAC GAC CTT TAT GGT CAA GTA GCA 1664 Val Leu Lys Leu He Asp Gin Tyr Asp Leu Tyr Gly Gin Val Ala 535 540 545 TAC CCC AAA CAT CAC AAG CAA GCT GAT GTT CCT GAG ATT TAT CGT 1709 Tyr Pro Lys His His Lys Gin Ala Asp Val Pro Glu He Tyr Arg 550 555 600 TTG GCA GCA AAG ACA AAG GGA GTC TTT ATT AAT CCA GCT TTT ATT 1754 Leu Ala Ala Lys Thr Lys Gly Val Phe He Asn Pro Ala Phe He 605 610 615 GAG CCA TTT GGG CTG ACT CTA ATA GAG GCA GCA GCT CAT GGT TTA 1799 Glu Pro Phe Gly Leu Thr Leu He Glu Ala Ala Ala His Gly Leu 620 625 630 CCG ATG GTT GCT ACG AAA AAT GGA GGC CCT GTT GAT ATC CAG AGG 1844 Pro Met Val Ala Thr Lys Asn Gly Gly Pro Val Asp He Gin Arg 635 640 645 GTC CTT GAT AAT GGT CTT CTT GTG GAT CCT CAT GAG CAG CAG TCT 1889 Val Leu Asp Asn Gly Leu Leu Val Asp Pro His Glu Gin Gin Ser 650 655 660 ATT GCT ACT GCT TTG CTG AAG CTT GTT GCT GAT AAG CAA CTA TGG 1934 He Ala Thr Ala Leu Leu Lys Leu Val Ala Asp Lys Gin Leu Trp 665 670 675 ACA AAA TGC CAG CAA AAT GGA CTG AAA AAT ATT CAT CTC TAC TCT 1979 Thr Lys Cys Gin Gin Asn Gly Leu Lys Asn He His Leu Tyr Ser 680 685 690 TGG CCA GAG CAT TCG AAG ACA TAC CTA TCT CGA ATA GCC AGT TCG 2024 Trp Pro Glu His Ser Lys Thr Tyr Leu Ser Arg He Ala Ser Ser 695 700 705 AGA CAA AGG CAA CCA CAG TGG CAA AGA AGT AGT GAT GAA GGG CTT 2069 Arg Gin Arg Gin Pro Gin Trp G n Arg Ser spr s l Gly Leu 710 715 720 GAC AAT CAA GAG CCT GAA TCT CCA AGT GAT TCT TTA AGA GAT ATA 2114 Asp Asn Gin Glu Pro Glu Ser Pro Ser Asp Ser Leu Arg Asp He 725 730 735 AAG GAT ATA TCT CTA AAC CTT GAA GTT CTC GTT AGA CCG GAG AAA 2159 Lys Asp He Ser Leu Asn Leu Glu Val Leu Val Arg Pro Glu Lys 740 745 750 AGG GTG AAG ACG TTG AAA ATC TTG GGA TTG ATG ACA AAA GCA AAT 2204 Arg Val Lys Thr Leu Lys He Leu Gly Leu Met Thr Lys Ala Asn 755 760 765 TCG AGA ATG CTG TTA TGT TCA TGG TCT AAT GGT GTC CAT AAG ATG 2249 Ser Arg Met Leu Leu Cys Ser Trp Ser Asn Gly Val His Lys Met 770 775 780 CTT CGG AAG GCT CGG TTC TCT GAC AAA GTA GAT CAG GCT TCT AGT 2294 Leu Arg Lys Ala Arg Phe Ser Asp Lys Val Asp ' Gin Ala Ser Ser 785 790 795 AAA TAT CCA GCA TTT AGG AGG AGA AAA CTT ATA TAT GTT ATT GCT 2339 Lys Tyr Pro Ala Phe Arg Arg Arg Lys Leu He Tyr Val He Ala 800 805 810 GTA GAC GGG GAT TAT GAA GAT GGA CTT TTT GAT ATT GTT CGG AGG 2384 Val Asp Gly Asp Tyr Glu Asp Gly Leu Phe Asp He Val Arg Arg 815 820 825 ATA TTT GAT GCT GCT GGC AAG GAG AAG ATT GAA GGT TCC ATC GGG 2429 He Phe Asp Ala Ala Gly Lys Glu Lys He Glu Gly Ser He Gly 830 835 840 TTT ATA TTG TCA ACA TCC TAT TCT ATG CCC GAA ATT CAG AAC TAT 2474 Phe He Leu Ser Thr Ser Tyr Ser Met Pro Glu He Gin Asn Tyr 845 850 855 TTG CTA TCA AAA GGC TTC AAT CTT CAT GAT TTT GAT GCA TAT ATA 2519 Leu Leu Ser Lys Gly Phe Asn Leu His Asp Phe Asp Ala Tyr He 860 865 870 TGC AAC AGT GGG AGT GAG TTG TAC TAT TCA TCT TTG AAC TCA GAG 2564 Cys Asn Ser Gly Ser Glu Leu Tyr Tyi Ser Ser Leu Asn Ser Glu 875 880 885 GAG AGT AAT ATT ATA GCA GAT TCA GAT TAC CAT TCA CAC ATA GAG 2609 Glu Ser Asn He He Ala Asp Ser Asp Tyr His Ser His He Glu 890 895 900 TAC AGA TGG GGT GGA GAA GGC CTT AGA AGG ACT TTG CTT CGC TGG 2654 Tyr Arg Trp Gly Gly Glu Gly Leu Arg Arg Thr Leu Leu Arg Trp 905 910 915 GCA GCT TCC ATC ACA GAA AAA AAT GGT GAA AAC GAA GAA CAG GTT 2699 Ala Ala Ser He Thr Glu Lys Asn Gly Glu Asn Glu Glu Gin Val 920 925 930 ATT ACT GAA GAT GAA GAA GTT TCT ACG GGT TAT TGC TTT GCG TTT 2744 He Thr Glu Asp Glu Glu Val Ser Thr Gly Tyr Cys Phe Ala Phe 935 940 945 AAA ATA AAG AAC CAA AAT AAG GTT CCC CCT ACG AAG GAG CTC CGC 2789 Lys He Lys Asn Gin Asn Lys Val Pro Pro Thr Lys Glu Leu Arg 950 955 960 AAG TCA ATG AGG ATT CAA GCT CTT CGT TGC CAT GTG ATT TAC TGT 2834 Lys Ser Met Arg He Gin Ala Leu Arg Cys His Val He Tyr Cys 965 970 975 CAG AAC GGA TCT AAA ATG AAT GTG ATT CCA GTA CTA GCA TCC CGT 2279 Gin Asn Gly Ser Lys Met Asn Val He Pro Val Leu Ala Ser Arg 980 985 990 TCT CAA GCC CTC AGG TAT CTT TAT GTT CGT TGG GGA GTT GAG TTG 2924 Ser Gin Ala Leu Arg Tyr Leu Tyr Val Arg Trp Gly Val Glu Leu 995 1000 1005 TCG AAG ATG GTT GTC TTT GTT GGA GAA TGT GGT GAC ACA GAT TAT 2969 Ser Lys Met Val Val Phe Val Gly Glu Cys Gly Asp Thr Asp Tyr 1010 1015 1020 GAA GGC TTG CTT GGC GGG GTC CAT AAA ACC GTA ATA CTG AAG GGA 3014 Glu Gly Leu Leu Gly Gly Val His Lys Thr Val He Leu Lys Gly 1025 1030 1035 GTC TCC AAC ACT GCT TTA AGG TCT CTC CAT GCC AAC AGA AGT TAC 3059 Val Ser Asn Thr Ala Leu Arg Ser Leu His Ala Asn Arg Ser Tyr 1040 1045 1050 CCT CTT TCA CAT GTC GTG TCG CTT GAC AGC CCC AAT ATT GGC GAG 3104 Pro Leu Ser His Val Val Ser Leu Asp Ser Pro Asn He Gly Glu 1055 1060 1065 GTG AGC AAA GGG TGC AGC AGC TCC GAG ATC CAG TCC ATC GTC ACA 3149 Val Ser Lys Gly Cys Ser Ser Ser Glu He Gin Ser He Val Thr 1070 1075 1080 AAA CTC TCC AAA GCT TAA TCAGATATCT GCTGCTTTCT TTTGGGTAAG 3197 Lys Leu Ser Lys Ala End 1085 CAAGGTTTCA TCTTATATGA TTATATCATA AGATACTATA TAAGCACCTT 3247 ATTGGTAAGT CAGTCCCATA ATAATAATGT ACTTCAGAAC CACAATACTT 3297 AAAAGTTGGT TCAGTAGTGA TTAGTCTCAT AATAATCATA TAATTACACA 3347 TCCGCTGTTA ACTAGTGGTA ATATCTAAGC TCAACAATAA AGATGTAAAA 3397 TGCTAGTATG GAAATGAATT GCTAGCTGTT GATCTCTTTC CCTTTATTCT 3447 GTATTATTTC TTTCCTCATC TCATGTAAAA ACAATTTTCT GAAGGTGTAC 3497 AGTTTTTTCC CCTTATATAT CTGTATT TT TCTACTATTT TTTGTTTGTA 3547 AGAATATCCT CTCATCGAGG AGTGATAATT AAATAACCGG CTTGCTAAAT 3597 ATAAAGCTTA TTCGAGTTAA AAAAAAAAAA AAAAAAAA 3635 4. DNA sequence with the coding region for sucrose-synthase for the preparation of sugar beet with modified sucrose ' concentration, characterised in that the sequence has the following nucleotide 5 sequence (Seq. ID No.4) : CT GCA GGA GGG AAA CAA ATT CTT AGC GAT GGC CCG TTT AGC GAA 0044 Ala Gly Gly Lys Gin He Leu Ser Asp Gly Pro Phe Ser Glu 5 10 GTT CTT AGG TCT GCT CAG GAA GCA ATA GTT GTT CCT CCC TTT GTT 0089 Val Leu Arg Ser Ala Gin Glu Ala He Val Val Pro Pro Phe Val 15 20 25 GCT ATA GCA GTC CGT CCA AGA CCT GGA GTT TGG GAA TAT GTT CGT 0134 Ala He Ala Val Arg Pro Arg Pro Gly Val Trp Glu Tyr Val Arg 30 35 40 GTT AAT GTC TCT GAA CTG AAT GTG GAG CAG CTA ACT GTG TCT GAG 0179 Val Asn Val Ser Glu Leu Asn Val Glu Gin Leu Thr Val Ser Glu 45 50 55 TAT CTC CAT TTC AAG GAA GAA CTT GTG GAT GGA AAG GCT GAT GAC 0224 Tyr Leu His Phe Lys Glu Glu Leu Val Asp Gly Lys Ala Asp Asp 60 65 70 CAC TAT GTG CTT GAG CTT GAT TTC GAG CCT TTT AAT GAA TCA GTT 0269 His Tyr Val Leu Glu Leu Asp Phe Glu Pro Phe Asn Glu Ser Val 75 80 85 CCA CGT CCA ACT CGC TCT TCA TCA ATT GGT AAT GGT GTT CAG TTC 0314 Pro Arg Pro Thr Arg Ser Ser Ser He Gly Asn Gly Val Gin Phe 90 95 100 CTC AAT CGG CAC CTG TCA TCA AGC ATG TTC TGC AAC AAA GAT TGC 035 Leu Asn Arg His Leu Ser Ser Ser Met Phe Cys Asn Lys Asp Cys 105 110 115 TTG GAG CCG TTA CTT GAT TTT CTT AGA GTG CAC AAA CAT AAA GGA 040 Leu Glu Pro Leu Leu Asp Phe Leu Arg Val His Lys His Lys Gly 120 125 130 GTT GTC ATG ATG TTG AAT GAT CGG ATA CAG ACT ATC CAG CGT CTT 044 Val Val Met Met Leu Asn Asp Arg He Gin Thr He Gin Arg Leu 135 140 145 CAG TCT GCA TTG TCT AAA GCT GAG GAT TAT CTT ATC AAA CTT CCA 049 Gin Ser Ala Leu Ser Lys Ala Glu Asp Tyr Leu He Lys Leu Pro 150 155 160 GCA GAT ACA CCT TAC TCT GAG TTC GAA TTT GTA ATC CAA GGT ATG 053 Ala Asp Thr Pro Tyr Ser Glu Phe Glu Phe Val He Gin Gly Met 165 170 175 GGT TTT GAA AGA GGC TGG GGT GAT ACT GCT GAA AGG GTT CTA GAA 058 Gly Phe Glu Arg Gly Trp Gly Asp Thr Ala Glu Arg Val Leu Glu 180 185 190 ATG ATG CAT CTA CTA CTA GAT ATC CTT CAG GCT CCC GAT CCG TCT 062 Met Met His Leu Leu Leu Asp He Leu Gin Ala Pro Asp Pro Ser 195 200 205 ACA TTA GAG ACA TTT CTG GGA AGA CTT CCC ATG GTG TTT AAT GTG 0674 Thr Leu Glu Thr Phe Leu Gly Arg Leu Pro Met Val Phe Asn Val 210 215 220 GTC ATT TTG TCT GTA CAT GGA TAT TTT GGA CAG GCA CAT GTG CTC 0719 Val He Leu Ser Val His Gly Tyr Phe Gly Gin Ala His Val Leu 225 230 235 GGC TTG CCT GAC ACT GGT GGG CAG ATA GTT TAT ATA CTT GAC CAA 0764 Gly Leu Pro Asp Thr Gly Gly Gin He Val Tyr He Leu Asp Gin 240 245 250 GTG CGG TCT CTG GAA CAT GAA ATG CTC CAA CGA ATA AAG AAG CAA 0809 Val Arg Ser Leu Glu His Glu Met Leu Gin Arg He Lys Lys Gin 255 260 265 GGA CTA GAT GTG ACT CCT AGA ATT CTT ATC GTG AGT CGG TTG ATT 0854 Gly Leu Asp Val Thr Pro Arg He Leu He Val Ser Arg Leu He 270 275 280 CCT GAC GCT AAA GGG ACC ACG TGC AAT CAA CGT ATG GAG AAA GTC 0899 Pro Asp Ala Lys Gly Thr Thr Cys Asn Gin Arg Met Glu Lys Val 285 290 295 AGT GGA ACA GAG CAT GCT AGT ATC CTG AGA GTT CCT TTC CGA TCA 0944 Ser Gly Thr Glu His Ala Ser He Leu Arg Val Pro Phe Arg Ser 300 305 310 GAG AAA GGA ATC CTC CGC AAA TGG ATA TCT AGA TTT GAT GTA TGG 098 Glu Lys Gly He Leu Arg Lys Trp He Ser Arg Phe Asp Val Trp 315 320 325 CCT TAT TTA GAG ACC TTC ACT GAG GAT GCA GCT GGT GAA ATT ATT 103 Pro Tyr Leu Glu Thr Phe Thr Glu Asp Ala Ala Gly Glu He He 330 335 340 GGC GAG TTG CAG GGT CGT CCA GAT CTG ATA ATT GGC AAC TAC AGC 107 Gly Glu Leu Gin Gly Arg Pro Asp Leu He He Gly Asn Tyr Ser 345 350 355 GAT GGG AAT ATA GTT GCT TCT TTA TTG TCC CAC AAA ATG GGT GTC 112 Asp Gly Asn He Val Ala Ser Leu Leu Ser His Lys Met Gly Val 360 365 370 ACC CAG TGC AAT ATA GCC CAT GCA TTG GAG AAA ACC AAG TAT CCA 116 Thr Gin Cys Asn He Ala His Ala Leu Glu Lys Thr Lys Tyr Pro 375 380 385 GAT TCT GAT ATT TAC TGG AAA AGA TTT GAG GAC AAA TAT CAC TTC 121 Asp Ser Asp He Tyr Trp Lys Arg Phe Glu Asp Lys Tyr His Phe 390 395 400 • TCG TGT CAA TTT TCA GCT GAC TTG ATG GCA ATG AAT CAT GCT GAT 125 Ser Cys Gin Phe Ser Ala Asp Leu Met Ala Met Asn His Ala Asp 405 410 415 TTC ATC ATT ACG AGT ACT TAC CAA GAG ATA GCT GGA ACG AAG AAT 1304 Phe He He Thr Ser Thr Tyr Gin Glu He Ala Gly Thr Lys Asn 420 425 430 ACT GTT GGT CAA TAT GAA AGC CAT AAG GCC TTT ACT TTT CCG GGG 1349 Thr Val Gly Gin Tyr Glu Ser His Lys Ala Phe Thr Phe Pro Gly 435 440 445 CTG TAT CGG GTG GTT CAC GGG ATT GAT GTC TTT GAT CCC AAG TTT 1394 Leu Tyr Arg Val Val His Gly He Asp Val Phe Asp Pro Lys Phe 450 455 460 AAT ATT GTC TCG CCA GGG GCA GAC ATG GCC ATC TAC TTC CCA TTT 1439 Asn He Val Ser Pro Gly Ala Asp Met Ala He Tyr Phe Pro Phe 465 470 475 TCA GAG AAG GAT GTC ACC TGT CTC ACT TCA CTT CAT AGA CTT ATA 1484 Ser Glu Lys Asp Val Thr Cys Leu Thr Ser Leu His Arg Leu He 480 485 490 GAG CAG CTC CTA TTC AAA CCT GAG CAG AAC GAA GAA CAC ATT GGT 1529 Glu Gin Leu Leu Phe Lys Pro Glu Gin Asn Glu Glu His He Gly 495 500 505 . GTA TTA GAT GAT ACC TCA AAG CCA ATT ATA TTT TCC ATG GCG AGG 1574 Val Leu Asp Asp Thr Ser Lys Pro He He Phe Ser Met Ala Arg 510 515 520 CTA GAC CGT GTG AAG AAT ATA ACA GGG CTG GTA GAG TGC TAT GGC 161 Leu Asp Arg Val Lys Asn He Thr Gly Leu Val Glu Cys Tyr Gly 525 530 535 AAG AAT GCG AAA CTC AGG GAA CTG GCA AAC CTG GTT GTA GTG GCT 166 Lys Asn Ala Lys Leu Arg Glu Leu Ala Asn Leu Val Val Val Ala 540 545 550 GGG TAC AAT GAT GTA AAA AAG TCG AAT GAC AGG GAG GAA ATT GCC 170 Gly Tyr Asn Asp Val Lys Lys Ser Asn Asp Arg Glu Glu He Ala 555 560 565 GAA ATC GAG AAG ATG CAC AGG CTT ATA CAG GAG TAT AAT TTA AGA 175 Glu He Glu Lys Met His Arg Leu He Gin Glu Tyr Asn Leu Arg 570 575 580 GGA CAA TTT CGC TGG ATT GCT TCT CAA ACA AAT AGA GTA CGA AAT 179 Gly Gin Phe Arg Trp He Ala Ser Gin Thr Asn Arg Val Arg Asn 585 590 595 GGT GAA CTC TAT CGC TAC ATT TGT GAC AAA GGA GGT ATT TTT GCG 184 Gly Glu Leu Tyr Arg Tyr He Cys Asp Lys Gly Gly He Phe Ala 600 605 610 CAG CCT GCA TTT TAT GAA GCA TTT GGG CTT ACA GTT GTT GAA GCC 188 Gin Pro Ala Phe Tyr Glu Ala Phe Gly Leu Thr Val Val Glu Ala 615 620 625 ATG ACC TGT GGT CTT CCC ACA TTT GCT ACC TGC CAC GGT GGT CCA 1934 Met Thr Cys Gly Leu Pro Thr Phe Ala Thr Cys His Gly Gly Pro 630 635 640 GCT GAG ATT ATA GAA GAC GGT GTT TCA GGA TTT CAT ATC GAT CCA 1979 Ala Glu He He Glu Asp Gly Val Ser Gly Phe His He Asp Pro 645 650 655 TAT CAT GCT GAT CAG GCA GAA AAA ATG ACT GAA TTC TTT GTC AAG 2024 Tyr His Ala Asp Gin Ala Glu Lys Met Thr Glu Phe Phe Val Lys 660 665 670 TGC AGA GAG GAT CCA AAC TAC TGG ACT AAA ATC TCT GCA GGA GGG 2069 Cys Arg Glu Asp Pro Asn Tyr Trp Thr Lys He Ser Ala Gay Gly 675 680 685 TTA CTA AGG ATC AAA GAA AGA TAT ACC TGG CAA AAG TAT TCT GAA 2114 Leu Leu Arg He Lys Glu Arg Tyr Thr Trp Gin Lys Tyr Ser Glu 690 695 700 AGG TTA ATG ACA TTG GCA GGG GTG TAT GGT TTC TGG AAA TAT GTC 2159 Arg Leu Met Thr Leu Ala Gly Val Tyr Gly Phe Trp Lys Tyr Val 705 710 715 TCT AAA CTA GAG AGA AGA GAG ACA CGA CGT TAT CTT GAG ATG TTC 2204 Ser Lys Leu Glu Arg Arg Glu Thr Arg Arg Tyr Leu Glu Met Phe 720 725 730 TAC ATT TTG AAG TTC CGT GAT CTG GCC AAC TCT GTT CCG CTG GCA 2249 Tyr He Leu Lys Phe Arg Asp Leu Ala Asn Ser Val Pro Leu Ala 735 740 745 ACA GAT GAA GAG CCT TCT ACT ACT GAT GCA GTT GCG ACA TTC CGT 2294 Thr Asp Glu Glu Pro Ser Thr Thr Asp Ala Val Ala Thr Phe Arg 750 755 760 GGA CCT TGA ACGCTGCTGC TTACTGAGGT TCCAAGTTGT GTATATATTA 2343 Gly Pro End CTGTGAAAGG AATAAGTGTA GCTACACAAA AGGTTCTCAA CTATTAGTAT 2393 CTTCTCTGTG TAAATAACGA GAGTGAAAAA TGTAATATTG TTGATGTCTT 2443 GAAAACTGAG TTTGCTTTGT TTATTTTTAA GTGTATGACA ATATGTATCA 2493 TATAACGGAT TCTTCAGTGA TCATATCAAA AACTACTGAC CATCGAAGTT 2543 AATGAAAATC GACAGCAACA 2563 5. Derivatives of DNA sequences according to any one of claims 1 to 4 characterised in that these derivatives are obtained by exchange of single bases 5 or by targeted or non-targeted mutagenesis. 6. Plasmids containing a) a suitable promoter which ensures that the coding sequence meets a suitable time point or 10 in the specified developments in the transgenic plant or in determined genes of transgenic plants; b) at least one coding sequence according to claims 1 to 5, that i) is so coupled to the promoter that the formation of an RNA is allowed which is into a protein, whereby the protein demonstrates an enzymatic activity which leads to a modification of the sucrose concentration in the plant, or ii) which is so coupled to the promoter that the coding part is read, which leads to the formation of a so-called anti-sense RNA which under-expresses the formation of the protein coded from an endogenous gene in the plant, that is involved in the sucrose biosynthesis; and c) A non-coding termination sequence that contains the signal for the termination and poly- adenylation of the transcript. 7. Use of the DNA-sequences according to any one of claims 1 to 6 for the preparation of sugar beet with changed sucrose concentration. 8. Use of the plasmids according to any one of claims 1-4 for the preparation of derivatives by targeted or non-targeted mutagenesis 9. Sugar beet containing a DNA sequence according to any one of claims 1-5.

Inventors: HESSE HOLGER, MUELLER-ROEBER BERND, HESSE, HOLGER, MUELLER-ROEBER, BERND
Applicants: HESSE HOLGER, HOECHST SCHERING AGREVO GMBH, MUELLER ROEBER BERND, HESSE, HOLGER, MUELLER-ROEBER, BERND
Application year: 1994
Main classification: C12N15
IPC codes: C12N9, C12N15