BIOCATALYTIC METHODS FOR SYNTHESIZING AND IDENTIFYING BIOLOGICALLY ACTIVE COMPOUNDS

Abstract: This invention encompasses methods for producing a library of modified starting compounds by use of biocatalytic reactions on a starting compound and identifying the modified starting compound with the optimum desired activity. The invention encompasses starting compounds and modified compounds that are free in solution. The method is useful in producing modified pharmaceutical compounds with desired specific activity.

Description: BIOCATALYTIC METHODS FOR SYNTHESIZING AND IDENTIFYING BIOLOGICALLY ACTIVE COMPOUNDS BACKGROUND OF THE INVENTION (a) Field of the Invention This invention is in the field of synthesizing and identifying biologically active compounds. (b) Description of the Prior Art The prior art is repleat with examples of chemically, microbially, or enzymatically synthesizing compounds with biological activity. The goal of these efforts is the discovery of new and improved pharmaceutical compounds. The discovery of new pharmaceutical compounds is for the most part a trial and error process. So many diverse factors constitute an effective pharmaceutical compound that it is extremely difficult to reduce the discovery process to a systematic approach. Typically, thousands of organic compounds must be isolated from biological sources or chemically synthesized and tested before a pharmaceutical compound is found. Synthesizing and testing new compounds for biological activity, which is the first step in identifying a new synthetic drug, is a time consuming and expensive undertaking. Typically, compounds must by synthesized, purified, tested and quantitatively compared to other compounds in order to identify active compounds or identify compounds with optimal activity. The synthesis of new compounds is accomplished for the most part using standard chemical methods. Such methods provide for the synthesis of virtually any type of organic compound; however, because chemical reactions are non-specific, these syntheses require numerous steps and multiple purifications before a final compound is produced and ready for testing. New biological and chemical approaches have recently been developed which provide for the synthesis and screening of large libraries of small peptides and oligonucleotides. These methods provide for the synthesis of a broad range of chemical compounds and provide the means to potentially identify biologically active compounds. The chemistries for synthesizing such large numbers of these natural and non-naturally occurring polymeric compounds is complicated, but manageable because each compound is synthesized with the same set of chemical protocols, the difference being the random order in which amino acids or nucleotides are introduced into the reaction sequence. Fodor, S.P.A. et al (1990) Science 251. 767-773, describe methods for discovering new peptide ligands that bind to biological receptors. The process combines solid-phase chemistry and photolithography to achieve a diverse array of small peptides. This work and related works are also described in Fodor WO Patent #9,210,092, Dower WO #9,119,818, Barrett WO #9,107,087 and Pirrung WO#9,015,070. Houghten, R.A. et al. (1991) Nature 354, 84-86, describe an approach that synthesizes libraries of free peptides along with an iterative selection process that permits the systematic identification of optimal peptide ligands. This work is also described in Appl. WO Patent #9,209,300. Lam, K.S., et al. (1991) Nature 354, 82-84, describe a method that provides for the systematic synthesis and screening of peptide libraries on a solid-phase microparticle support on the basis of a 'one-bead, one-peptide' approach. Cwirla, S.E., et al (1990) Proc. Natl. Acad. Sci. USA 87, 6378-6382, describe a method for constructing a library of peptides on the surface of a phage by cloning randomly synthesized oligonucleotides into the 5' region of specific phage genes resulting in millions of different hexapeptides expressed at the N terminus of surface proteins. These methods accelerate the identification of biologically active peptides and oligonucleotides. However, peptides and oligonucleotides have poor bioavailability and limited stability in vivo, which limits their use as therapeutic agents. In general, non-biological compounds which mimic the structure of the active peptides and oligonucleotides must be synthesized based on the approximated three dimensional structure of the peptide or oligonucleotide and tested before an effective drug structure can be identified. Bunin et al., J. Am. Chem. Soc. (1992) 114. 10997-10998 describe the synthesis of numerous 1,4 benzodiazapine derivatives using solid phase synthesis techniques. The prior art is repleat with examples showing enzymatic conversion of non-physiological substances under many conditions. References demonstrating that enzyme specificity can be changed/tailored: 1. Zaks, A. and Klibanov, A.M. Substrate specificity of enzymes in organic solvents vs. water is reversed. Journal of the American Chemical Society i_ 8 2767-2768, 1986. 2. Ferjancic, A., Puigserver, A. and Gaertner, H. Unusual specificity of PEG-modified thermolysin in peptide synthesis catalyzed in organic solvents. Biotechnology Letters 10 (2) 101- 106, 1988. 3. Nasri, M. and Thomas, D. Increase of the potentialities of restriction endonuleases by specificity relaxation in the presence of organic solvents. Ann. N.Y. Acad. Sci. 542 255-265, 1988. 4. Stahl, M., Mansson, M.O. and Mosbach, K. The synthesis of a D-amino acid ester in an organic media with chymotrypsin modified by a bio-imprinting procedure. Biotechnology Letters 12 (3) 161-166, 1990. 5. Stahl, M., Jeppsson-Wistrand, U., Mansson, M.O. and Mosbach, K. Induced stereoselectivity and substrate selectivity of bio-imprinted a-chymotrypsin in anhydrous organic media. Journal of the American Chemical Society 113 (24) 9366-9368, 1991. 6. Gololobov, M.Y., Voyushina, T.L., Stepanov, V.M. and Adlercreutz, P. Organic solvent changes the chymotrypsin specificity with respect to nucleophiles. FEBS Letters 307 (3) 309-312, 1992. 7. Hertmanni, P., Pourplanche, C. and Larreta-Garde, V. Orientation of enzyme catalysis and specificity by water-soluble additives. Ann. New York Acad.Sci. (Enzyme Eng. XI, D.S. Clark, D.A. Estell, eds) 672 329-335, 1992. 8. Cabezas, M.J., del Campo, C, Llama, E., Sinisterra, J.V. and Gaertner, H. Organic reactions catalysed by modified enzymes. 1. Alteration of the substrate specificity of a- chymotrypsin by the modification process. Journal of Molecular Catalysis 21 (2) 261-278, 1992. 9. Nagashima, T., Watanabe, A. and Kise, H. Peptide synthesis by proteases in organic solvents: medium effect on substrate specificity. Enzyme and Microbial Technology 14 (10) 842-847, 1992. 10. Parida, S. and Dordick, J.S. Tailoring lipase specificity by solvent and substrate chemistries, J. Org. Chem. 5 . (12) 3238-3244, 1993. 11. Tawaki, S. and Klibanov, A.M. Chemoselectivity of enzymes in anhydrous media is strongly solvent dependent. Biocatalysis £ (1) 3-19, 1993. 12. Wescott, CF. and Klibanov, A.M. Solvent variation inverts substrate specificity of an enzyme. JACS 1 (5) 1629-1631, 1993. References demonstrating that enzyme enantioselectivity can be changed/tailored: 1. Sakurai, T., Margolin, A.L., Russell, A.J. and Klibanov, A. M. Control of enzyme enantioselectivity by the reaction medium. Journal of the American Chemical Society JJO (21) 7236-7237, 1988. 2. Fitzpatrick, P.A. and Klibanov, A.M. How can the solvent affect enzyme enantioselectivity? Journal of the American Chemical Society H^ (8) 3166-3171, 1991. 3. Hult, K. and Norin, T. Enantioselectivity of some Upases - control and prediction. Pure and Applied Chemistry 64 (8) 1129-1134, 1992. 4. Miyazawa, T., Kurita, S., Ueji, S., Yamada, T. and Kuwata, S. Resolution of racemic carboxylic acids via the lipase-catalyzed irreversible transesterification using vinyl esters - effects of alcohols as nucleophiles and organic solvents on enantioselectivity. Biotechnology Letters 14 (10) 941-946, 1992. 5. Tawaki, S. and Klibanov, A.M. Inversion of enzyme enantioselectivity mediated by the solvent Journal of the American Chemical Society 114 (5) 1882-1884, 1992. 6. Ueji, S., Fujino, R., Okubo, N., Miyazawa, T., Kurita, S., Kitadani, M. and Muromatsu, A. Solvent-induced inversion of enantioselectivity in lipase-catalyzed esterification of 2-phenoxypropionic acids. Biotechnology Letters 14 (3) 163-168, 1992. 7. Terradas, F., Testonhenry, M., Fitzpatrick, P.A. and Klibanov, A.M. Marked dependence of enzyme prochiral selectivity on the solvent. Journal of the American Chemical Society 115 (2) 390-396, 1993. 8. Herradon, B. Biocatalytic synthesis of chiral polyoxygenated compounds: effect of the solvent on the enantioselectivity of Upase catalyzed transesterifications in organic solvents. Synlett 2 108-110, 1993. References demonstrating the ability of enzymes to convert unnatural substrates: 1. Bianchi, D., Cesti, P., Golini, P., Spezia, S., Garavaglia, C. and Mirenna, L. Enzymatic preparation of optically active fungicide intermediates in aqueous and in organic media. Pure and Applied Chemistry 64 (8) 1073-1078, 1992. 2. Natoli, M., Nicolosi, G. and PiattelU, M. Regioselective alcoholysis of flavonoid acetates with lipase in an organic solvent. Journal of .Organic Chemistry 57 (21) 5776-5778, 1992. 3. Izumi, T., Tamura, F. and Sasaki, K. Enzymatic kinetic resolution of <4>(l,2)fenocenophane derivatives. Bulletin of the Chemical Society of Japan 6J5. (10) 2784- 2788, 1992. 4. Miyazawa, T., Mio, M., Watanabe, Y., Yamada, T. and Kuwata, S. Lipase- catalyzed transesterification procedure for the resolution of non-protein amino acids. Biotechnology Letters 14 (9) 789-794, 1992. 5. Murata, M., Uchida, H. and Achiwa, K. Lipase-catalyzed enantioselective synthesis of optically active mephobarbital, hexobarbital and febarbamate. Chemical- Pharmaceutical Bulletin 4Q (10) 2605-2609, 1992. 6. Johnson, C.R., Golebiowski, A. and Steensma, D.H. Enzymatic asymmetrization in organic media - synthesis of unnatural glucose from cycloheptatriene. Journal of the American Chemical Society 114 (24) 9414-9418, 1992. 7. Cruces, M.A., Otero, C, Bernabe, M., Martinlomas, M. and Ballesteros, A. Enzymatic preparation of acylated sucroses. Ann. New York Acad.Sci. (Enzyme Eng. XI, D.S. Clark, D.A. Estell, eds) £72 436-443, 1992. 8. Tanaka, A., Fukui, T., Uejima, A., Zong, M.H. and Kawamoto, T. Bioconversion of nonnatural organic compounds - esterification and dehydrogenation of organosilicon compounds. Ann. New York Acad. Sci (Enzyme Eng. XI, D.S. Clark, D.A. Estell, eds) £72431-435, 1992. 9. Kodelia, G. and Kolisis, F.N. Studies on the reaction catalyzed by protease for the acylation of flavonoids in organic solvents. Ann. New York Acad.Sci (Enzyme Eng. XI, D.S. Clark, D.A. Estell, eds) £72 451-457, 1992. 10. Wagner, F., Kleppe, F., Lokotsch, W., Ziemann, A. and Lang, S. Synthesis of uncommon wax esters with immobilized Upases. Ann. New York Acad.Sci. (Enzyme Eng. XI, D.S. Clark, D.A. Estell, eds) 672 484-491, 1992. 11. Patel. R.N., Howell, J.M., Banerjee, A., Fortney, K.F. and Szarka, L. J. Stereoselective enzymatic esterification of 3-benzoylthio-2-methylpropanoic acid. Ann. New York Acad. Sci (Enzyme Eng. XI, D.S. Clark, D.A. Estell, eds) £72 415-424, 1992. 12. Bergbreiter, D.E. and Momongan, M. Asymmetric synthesis of organometallic reagents using enzymatic methods. Applied Biochemistry and Biotechnology 32 1-3 55-72, 1992. 13. Carretero, J.C. and Dominguez, E. Lipase-catalyzed kinetic resolution of _- hydroxy phenyl sulfones. Journal of Organic Chemistry £7 (14) 3867-3873, 1992. 14. Johnson, C.R., Adams, J.P., Bis, S.J., Dejong, R.L., Golebiowski, A., Medich, J. R., Penning, T.D., Senanayake, C.H., Steensma, D.H. and Vanzandt, M.C. Applications of enzymes in the synthesis of bioactive polyols. Indian Journal of Chemistry Section B - Organic Chemistry Including Medicinal Chemistry 32 (1) 140-144, 1993. 15. Fabre, J., Betbeder, D., Paul, F., Monsan, P. and Perie, J. Regiospecific enzymic acylation of butyl a-D-glucopyranoside. Carbohydrate Research 243 (2) 407-411, 1993. 16. Bianchi, D., Cesti, P., GoUni, P., Spezia, S., Garavaglia, C. and Mirenna, L. Enzymatic preparation of optically active fungicide intermediates in aqueous and in organic media. Indian Journal of Chemistry Section B - Organic Chemistry Including Medicinal Chemistry 32 (1) 176-180, 1993. 17. Kanerva, L.T. and Sundholm, O. Lipase catalysis in the resolution of racemic intermediates of diltiazem synthesis in organic solvents. Journal of the Chemical Society - Perkin Transactions 1 13 1385-1389, 1993. 18. Ikeda, I. and Klibanov, A.M. Lipase-catalyzed acylation of sugars solubiUzed in hydrophobic solvents by complexation. Biotechnology and Bioengineering 42(6) 788-791, 1993. 19. Hyun, C.K., Kim, J.H. and Ryu, D.D.Y. Enhancement effect of water activity on enzymatic synthesis of cephalexin. Biotechnology and Bioengineering 42(7) 800-806, 1993. 20. Panza, L., Luisetti, M., Crociati, E. and Riva, S. Selective acylation of 4,6-O- benzylidene glycopyranosides by enzymatic catalysis. J. Carbohydrate Chem. 12 (1) 125-130, 1993. 21. Wang, L., Kobatake, E., Ikariyama, Y. and Aizawa, M. Regioselective oxidative polymerization of 1,5- dihydroxynaphthalene catalyzed by bilirubin oxidase in a water-organic solvent mixed solution. Journal of Polymer Science Part A - Polymer Chemistry H (11) 2855- 2861, 1993. 22. Knani, D. and Kohn, D.H. Enzymatic polyesterification in organic media. 2. Enzyme-catalyzed synthesis of lateral-substituted aUphatic polyesters and copolyesters. J. Polymer Sci., Part A - Polymer Chem. 21 (12) 2887-2897, 1993. 23. Kanerva, L.T. and Sundholm O. Enzymatic acylation in the resolution of methyl threo-2-hydroxy-3-(4-methoxyphenyl)-3-(2-x-phenylthio) propionates in organic solvents. Journal of the Chemical Society - Perkin Transactions 1202407-2410, 1993. 24. Sharma, A. and Chattopadhyay, S. Lipase catalysed acetylation of carbohydrates. Biotechnology Letters 11 (11) 1145-1146, 1993. 25. Pavel, K. and Ritter, H. Enzymes in polymer chemistry. 7. Lipase-catalyzed esterification of carboxyl-terminated methacrylic oUgomers and copolymers with isopropyl alcohol and 9-fluorenylmethanol. Makromolekulare Chemie- Macromolecular Chemistry and Physics 194 (12) 3369-3376, 1993. 26. Uejima, A., Fukui, T., Fukusaki, E., Omata, T., Kawamoto, T., Sonomoto, K. and Tanaka, A. Efficient kinetic resolution of organosilicon compounds by stereoselective esterification with hydroiases in organic solvent Appl. Microbiol.Biotech. 3 i (4) 482-486, 1993. 27. Mukesh, D., Sheth, D., Mokashi, A., Wagh, J., Tilak, J.M., Banerji, A.A. and Thakkar, K.R. Lipase catalysed esterification of isosorbide and sorbitol. Biotechnology Letters 11 (12) 1243-1246, 1993. 28. Baldessari, A., Iglesias, L.E. and Gros, E.G. Regioselective acylation of 3- mercaptopropane-l,2-diol by lipase-catalysed transesterification. Journal of Chemical Research - S 9 382-383, 1993. 29. De Goede, A.T.J.W., Benckhuijsen, W., van Rantwijk, F., Maat, L. and van Bekkum, H. Selective lipase-catalyzed 6-O-acylation of alkyl a-D-glucopyranosides using functionalized ethyl esters. Recueil Des Travaux Chimiques Des Pays Bas - Journal of the Royal Netherlands Chemical Society 112 (11) 567-572, 1993. 30. Menendez,E. and Gotor, V. Acylation and alkoxycarbonylation of oximes through an enzymatic oximolysis reaction. Synthesis - Stuttgart 172-74, 1993. 31. Li, Y.F. and Hammerschmidt, F. Enzymes in organic chemistry. 1. Enantioselective hydrolysis of a-(acyloxy) phosphonates by esterolytic enzymes. Tetrahedron - Asymmetry 4 (1) 109-120, 1993. 32. Schlotterbeck, A., Lang, S., Wray, V. and Wagner, F. Lipase-catalyzed monoacylation of fructose. Biotechnology Letters 1£ (1) 61-64, 1993. 33. Frykman, H., Ohrner, N., Norm, T. and Hult, K. S-Ethyl thiooctanoate as acyl donor in lipase catalysed resolution of secondary alcohols. Tetrahedron Letters __4 (8) 1367-1370, 1993. 34. Oguntimein, G.B., Erdmann, H. and Schmid, R.D. Lipase catalysed synthesis of sugar ester in organic solvents. Biotechnology Letters 15 (2) 175-180, 1993. 35. Lopez, R., Perez, C, Fernandez-Mayorales, A. and Conde, S. Enzymatic transesterification of alkyl 2,3,4-tri-O-acyl-beta-D-xylopyranosides. J. Carbohydrate Chem. 12 (2) 165-171, 1993. 36. Naemura, K., Ida. H. and Fukuda, R. Lipase YS-catalyzed enantioselective transesterification of alcohols of bicarbocyclic compounds. BuU. Chem. Soc.Japan 6£ (2) 573- 577, 1993. 37. Lambusta, D., Nicolosi, G., PiattelU, M. and Sanfilippo, C. Lipase catalyzed acylation of phenols in organic solvents. Ind. J .Chem. Section B 22. (1) 58-60, 1993. 38. Astorga, C, Rebolledo, F. and Gotor, V. Enzymatic hydrazinolysis of diesters and synthesis of N-aminosuccinimide derivatives. Synthesis - Stuttgart 2287-289, 1993. 39. Fukui, T., Kawamoto, T. and Tanaka, A. Enzymatic preparation of optically active silylmethanol derivatives having a stereogenic silicon atom by hydrolase-catalyzed enantioselective esterification. Tetrahedron - Asymmetry 5 (1) 73-82, 1994. 40. Vazquez-Duhalt, R., Westlake, D.W.S. and Fedorak, P.M. Lignin peroxidase oxidation of aromatic compounds in systems containing organic solvents. Applied and Environmental Microbiology £0 (2) 459-466, 1994. 41. Kodelia, G., Athanasiou, K. and Kolisis, F.N. Enzymatic synthesis of butyryl- rutin ester in organic solvents and its cytogenetic effects in mammaUan ceUs in culture. Appl. Biochem. Biotech. 44 (3) 205-212, 1994. 42. Tsai, S.W. and Wei, H.J. Effect of solvent on enantioselective esterification of naproxen by Upase with trimethylsilyl methanol. Biotechnology and Bioengineering 42 (1) 64-68, 1994. 43. Athawale, V.D. and Gaonkar, S.R. Enzymatic synthesis of polyesters by Upase catalysed polytransesterification. Biotechnology Letters 1£ (2) 149-154, 1994. 44. Janssen, G.G. and Haas, MJ. Lipase-catalyzed synthesis of oleic acid esters of polyethylene glycol 400. Biotechnology Letters 16 (2) 163-168, 1994. 45. Kitazuma, T., D eya, T. and Murata, K. Synthesis of optically active trifluorinated compounds: asymmetric Michael additional with hydrolytic enzymes. Journal of Chemical Society, Chemical Communications 1331, 1986. References demonstrating microbial transformations: 1. Holland, H.L. Fungi as reagents in organic synthesis: preparation of some metabolites of prostanoids, steroids, and other natural products. Rev. Latinoam. Quim., (1st Spec. SuppL), 318-329, 1990. 2. Sih, C.J. and Rosazza, J.P. Microbial transformations in organic synthesis. Tech. Chem. (N.Y.), JO (Appl. Biochem. Syst. Org. Chem., Part 1) 69-106, 1976. 3. Sih, C.J. and Chen, C.S. Microbial asymmetric catalysis-enantioselective reduction of ketones. Angew. Chem. Int. Ed. Engl. 23 (8) 570-578, 1984. 4. Thompson, L.A. and Knowles, C.J., Linton, E.A. and Wyatt, J.M. Microbial biotransformations of nitriles. Chem. Br. 4 (9) 900, 1988. 5. Servi, S. Baker's yeast as a reagent in organic synthesis. Synthesis 1 1-25, 1990. 6. Csuk, R. and Glanzer, B.I. Baker's yeast mediated transformations in organic chemistry. Chem. Rev. £1 (1) 49-97, 1991. 7. Roberts, S.M., Wiggins, K. and Casy, G. Whole cells and isolated enzymes in organic synthesis. Wiley, New York, 1992. 8. Ward, O.P. and Young, C.S. Reductive biotransformations of organic compounds by ceUs or enzymes of yeast. Enxyme Microb. Technol.12 (7) 482-493, 1990. Reviews: 1. Jones, J.B. Enzymes in organic synthesis. Tetrahedron 42 (13) 3351-3405, 1986. 2. Yamada, H. and Shimizu, S. Microbial and enzymatic processes for the production of biologically and chemically useful compounds. Angew. Chem. Int. Ed. Engl. 22 (5) 622-642, 1988. 3. Roberts, S.M. Enzymes as catalysts in organic synthesis. NATO ASI Ser., Ser. A. 128 443-463, 1989. 4. Chen, S.S. and Sih, C.J. General aspects and optimization of enantioselective biocatalysis in organic solvents: the use of Upases. Angew. Chem (Int. Ed. Engl. 28, n 6 695- 707) 101 (6) 711-724, 1989. SUMMARY OF THE INVENTION The present invention is used to synthesize a library of non-biological organic compounds from a starting compound and identify individual compounds within the library which exhibit biological activity. Unlike peptides and oUgonucleotides, non-biological organic compounds comprise the bulk of proven therapeutic agents. The invention can be used to directly identify new drug candidates or optimize an established drug compound which has sub-optimal activity or problematic side effects. This is accomplished dirough the use of highly specific biocatalytic reactions. Enzymes are highly selective catalysts. Their hallmark is the ability to catalyze reactions with exquisite stereo-, regio-, and chemo-selectivities that are unparalleled in conventional synthetic chemistry. Moreover, enzymes are remarkably versatile. They can be tailored to function in organic solvents, operate at extreme pH's and temperatures, and catalyze reactions with compounds that are structurally unrelated to their natural, physiological substrates. Enzymes are reactive toward a wide range of natural and unnatural substrates, thus enabling the modification of virtually any organic lead compound. Moreover, unlike traditional chemical catalysts, enzymes are highly enantio- and regio-selective. The high degree of functional group specificity exhibited by enzymes enables one to keep track of each reaction in a synthetic sequence leading to a new active compound. Enzymes are also capable of catalyzing many diverse reactions unrelated to their physiological function in nature. For example, peroxidases catalyze the oxidation of phenols by hydrogen peroxide. Peroxidases can also catalyze hydroxylation reactions that are not related to die native function of the enzyme. Other examples are proteases which catalyze the breakdown of polypeptides. In organic solution some proteases can also acylate sugars, a function unrelated to the native function of these enzymes. The present invention exploits the unique catalytic properties of enzymes. Whereas the use of biocatalysts (i.e., purified or crude enzymes, non-living or living cells) in chemical transformations normally requires the identification of a particular biocatalyst that reacts with a specific starting compound, the present invention uses selected biocatalysts and reaction conditions that are specific for functional groups that are present in many starting compounds. Each biocatalyst is specific for one functional group, or several related functional groups, and can react with many starting compounds containing this functional group. The biocatalytic reactions produce a population of derivatives from a single starting compound. These derivatives can be subjected to another round of biocatalytic reactions to produce a second population of derivative compounds. Thousands of variations of the original compound can be produced with each iteration of biocatalytic derivatization. Enzymes react at specific sites of a starting compound without affecting the rest of the molecule, a process which is very difficult to achieve using traditional chemical methods. This high degree of biocatalytic specificity provides die means to identify a single active compound witiiin the library. The library is characterized by the series of biocatalytic reactions used to produce it, a so called "biosynthetic history". Screening the library for biological activities and tracing the biosynthetic history identifies the specific reaction sequence producing the active compound. The reaction sequence is repeated and the structure of the synthesized compound determined. This mode of identification, unlike other synthesis and screening approaches, does not require immobilization technologies, and compounds can be synthesized and tested free in solution using virtually any type of screening assay. It is important to note, that the high degree of specificity of enzyme reactions on functional groups allows for the "tracking" of specific enzymatic reactions that make up the biocatalytically produced Ubrary. Many of the procedural steps are performed using robotic automation enabling the execution of many thousands of biocatalytic reactions and screening assays per day as well as ensuring a high level of accuracy and reproducibiUty. As a result, a library of derivative compounds can be produced in a matter of weeks which would take years to produce using current chemical methods. The present invention is unique in that it involves a soluble state of the starting compound and its subsequent derivatives. This is a highly unique aspect of the present invention that has been thought to be a barrier. Previous organic modifying technologies for biologically active compound identification involve starting compounds and derivatives attached to insoluble supports. This is taught in examples by EUman (Bunin, B.S.; Ellman, J.A. "A general and expedient method for the soUd-phase synthesis of 1,4-benzodiazepine derivatives", J. Am. Chem. Soc. 1992, 114 10997-10998), Gordon et al., (Gordon, E.M.; Barrett, R.W.; Dower, W.J.; Fodor, S.P.A.; GaUop, M.A. "Applications of Combinatorial Technologies to Drug Discovery, 1. Background and Peptide Combinatorial Libraries", J. Med. Chem. 1994, 22 1233-1251; and Gordon, E.M.; Barrett, R.W.; Dower, W.J.; Fodor, S.P.A.; Gallop, M.A. "Applications of Combinatorial Technologies to Drug Discovery, 2. "Combinatorial Organic Synthesis, Library Screening Strategies, and Future Directions", J. Med. Chem. 1994, 22 1385-1401), Hobbs Dewitt et al., (Hobbs Dewitt, S.; Kiely, J.S.; Stankovic, C.J.; Schroeder, M.C.; Reynolds Cody, D.M.; Pavia, M.R. "Diversomers: An approach to nonpeptide, nonoUgomeric chemical diversity" P.N.A.S. (USA) 1993 90 6909-6913). In addition to synthesising the libraries on a soluble state, the libraries of the instant invention can then be screened immediately witiiout removal of the libraries from solid supports. Thus by maintaining the components in the soluble state, the present invention provides for useful, convienent, and efficient methods of generating and screening libraries of starting compounds and derivatives. The present invention specifically incorporates a number of diverse technologies such as: (1) the use of enzymatic reactions to produce a library of drug candidates; (2) the use of enzymes free in solution or immobilized on the surface of particles, and organic compounds derivatized while dissolved in solution; (3) die use of receptors (hereinafter this term is used to indicate true receptors, enzymes, antibodies and other biomolecules which exhibit affinity toward biological compounds, and otiier binding molecules to identify a promising drug candidate within a library, even where such receptors are still associated with cell membranes, or intact ceUs); (4) the automation of all biocatalytic processes and many of the procedural steps used to test the Ubraries for desired activities, and (5) the coupUng of biocatalytic reactions with drug screening devices which can immediately measure the binding of synthesized compounds to receptor molecules or in whole-cell assays and thereby immediately identify specific reaction sequences giving rise to biologicaUy active compounds. Specifically, the present invention encompasses a method for drug identification comprising: (a) conducting a series of biocatalytic reactions by mixing biocatalysts with a starting compound to produce a reaction mixture and thereafter a Ubrary of modified starting compounds; (b) testing the library of modified starting compounds to determine if a modified starting compound is present witiiin the library which exhibits a desired activity; (c) identifying the specific biocatalytic reactions which produce the modified starting compound of desired activity by systematicaUy eliminating each of the biocatalytic reactions used to produce a portion of die Ubrary and testing the compounds produced in the portion of die library for the presence or absence of the modified starting compound with the desired activity; and (d) repeating the specific biocatalytic reactions which produce die modified compound of desired activity and determining the chemical composition of the reaction product. More specifically, the enzymatic reactions are conducted with a group of enzymes that react with distinct structural moieties found within the structure of a starting compound. Each enzyme is specific for one structural moiety or a group of related structural moieties. Furthermore, each enzyme reacts with many different starting compounds which contain die distinct structural moiety. In addition to systematically eliminating each reaction, the instant invention also provides for the systematic process of building up each reaction, an approach from the other end of die pathway. A particular feature of the instant invention is the soluble state of the starting compounds and die subsequent derivatives. BRIEF DESCRIPΗON OF THE DRAWINGS Figure 1 shows the starting active compound AZT with four potential sites for biocatalytic derivatization and eight possible biocatalytic reactions that can be used to produce a Ubrary of derivative compounds. Figure 2 shows an automated system employing robotic automation to perform hundreds of biocatalytic reactions and screening assays per day. Figure 3 illustrates the tracking of biocatalytic reactions to identify the sequence of reactions producing an active compound, which can subsequendy be used to produce and identify die structure of the active compound. Figure 4a iUustrates biocatalytic modification of castanospermine. Figure 4b illustrates biocatalytic modifications of methotrexate. DETAILED DESCRIPΗON OF THE INVENTION While the invention will be described in connection widi certain preferred embodiments, it will be understood that the description does not limit the invention to these particular embodiments. The embodiements of the invention are described with AZT, and further described for taxol, only as particular embodiments of die instant invention. In fact, it is to be understood that all alternatives, modifications and equivalents are included and are protected, consistent witii the spirit and scope of the inventions as defined in the appended claims. The preferred embodiments of die invention are set forth in the following example: a) A starting compound such as AZT (3'-azidothymidine), is chosen which exhibits drug activity or is believed to exhibit drug activity for a given disease or disorder. The compound is analyzed with respect to its functional group content and its potential for structural modifications using selected biocatalytic reactions. Functional groups which can be chemically modified using die selected biocatalytic reactions are listed in Table I. One of more of these functional groups are present in virtually all organic compounds. A partial list of possible enzymatic reactions that can be used to modify these functional groups is presented in Table II. b) A strategy is developed to systematically modify tiiese functional groups using selected biocatalytic reactions and produce a Ubrary of derivative compounds to be screened for biological activity. AZT contains four functional groups which are selected for biocatalytic modification: a primary hydroxyl, two carbonyls and a tertiary amine. The biosynthetic strategy is designated in the form of biocatalytic "reaction box" numbers which correspond to specific types of biocatalytic reactions acting on specific functional groups present in the starting compound. These "reaction boxes" are listed in Table III. The following biocatalytic "reaction boxes" are selected to synthesize an AZT derivative library: A3, A10, Al 1, C2, G6, G10 and G12. FIG. 1 illustrates the reaction of AZT with these selected biocatalytic reaction boxes. c) The biocatalytic reaction boxes are entered into an automated system which is shown in FIG 2. The system is programmed to automatically execute the aforementioned biocatalytic reactions and synthesize a Ubrary of derivative products. A single automated system in capable of performing hundreds of pre-programmed biocatalytic reactions per day. We can estimate die total number of compounds that can be produced by analyzing the reaction products produced in each "reaction box" and multiplying die results. Table IV details die number of potential reaction products produced in each reaction box and the resulting total number of possible compounds produced. In the case of AZT, up to 1.75 x 10* 1 new compounds can be synthesized. It should be pointed out that this compares very favorably to peptide libraries. For example, a library of hexapeptides will contain 20^ or 64 million compounds. This is a mere fraction, about 0.04% of the compounds that are possible using the biosynthetic approach described herein. Table V lists die results of a similar analysis on eleven other starting drug compounds. As shown in tiiis table, the biocatalytic reactions can generate huge numbers of derivative compounds for drug screening. d) The synthesized library of new compounds is assayed using enzyme inhibition assays, receptor- binding assays, immunoassays, and/or cellular assays to identify biologically active compounds. Before assaying die library of derivative compounds, any remaining AZT present in the Ubrary is either removed or inhibited to simplify the interpretation of screening assay results. This is easily accompUshed by HPLC, TLC, or the addition of a monoclonal antibody specific for the starting compound. Numerous in vitro assays are available that test for anti-viral, anti-cancer, anti- hypertensive and other well known pharmacological activities. Some of these assays are listed in Table VI. Most of tiiese assays are also performed on the automated system. e) Libraries which test positive are further analyzed using a biocatalytic tracking protocol which quickly identifies the specific sequence of reactions responsible for the synthesis of the compound testing positive in the screening assay. The high degree of specificity exhibited by biocatalysts, i.e., the ability of a given enzyme to react with a given functional group, enables this approach to be easily performed. The library is characterized by the series of biocatalytic reactions used to produce it, a so called "biosynthetic history". Portions of the Ubrary are screened for biological activity until the specific reaction sequence producing the active compound is identified. FIG. 3 illustrates this tracking process. For example, the dark line path 15. illustrates the reaction pathway to the most active compound. The reaction sequence is repeated to produce a sufficient amount of product for chemical analysis. The specificity of the biocatalytic reactions also permits the accurate duplication of the reaction pathway producing die active compounds. The structure of the active compound is qualitatively determined by analyzing the starting compounds, substrates and identified biocatalytic reaction sequence. The structure is then confirmed using gas chromotography, mass spectroscopy, NMR spectroscopy and other organic analytical methods. This mode of identification eliminates the need for product purification and also reduces d e amount of test screening required to identify a promising new drug compound. This process dramatically reduces the time necessary to synthesize and identify new drug compounds. In addition, tiiis mode of active compound identification does not require immobilization technologies, and compounds can be synthesized and tested free in solution under in vivo like conditions using virtually any type of screening assay (receptor, enzyme inhibition, immunoassay, cellular, animal model). Those skilled in the pharmaceutical arts wiU recognize the large number of biocatalytic conversions such as those listed in Table II and Table III, as well as the in vitro drug screening assays listed in Table VI. Those skilled in the pharmaceutical arts will recognize that biocatalytic reactions are optimized by controlling or adjusting such factors as solvent, buffer, pH, ionic strength, reagent concentration and temperature. The biocatalysts used in the biocatalytic reactions may be crude or purified enzymes, cellular lysate preparations, partially purified lysate preparations, living cells or intact non-Uving ceUs, used in solution, in suspension, or immobiUzed on magnetic or non-magnetic surfaces. In addition, non-specific chemical reactions may also be used in conjunction with the biocatalytic reaction to obtain the library of modified starting compounds. Examples of such non¬ specific chemical reactions include: hydroxylation of aromatics and aliphatics; oxidation reactions; reduction reactions; hydration reactions; dehydration reactions; hydrolysis reactions; acid/based catalyzed esterification; transesterification; aldol condensation; reductive amination; arnminolysis; dehydrohalogenation; halogenation; acylation; acyl substitution; aromatic substitution; Grignard synthesis; Friedel-Crafts acylation; etiierification. The biocatalytic reaction can be performed with a biocatalyst immobilized to magnetic particles forming a magnetic biocatalyst. The method of this embodiment is performed by initiating the biocatalytic reaction by combining the immobiUzed biocatalyst with substrate(s), cofactors(s) and solvent/buffer conditions used for a specific biocatalytic reaction. The magnetic biocatalyst is removed from the biocatalytic reaction mixture to terminate the biocatalytic reaction. This is accompUshed by applying an external magnetic field causing the magnetic particles widi the immobiUzed biocatalyst to be attracted to and concentrate at the source of the magnetic field, tiius effectively separating the magnetic biocatalyst from the bulk of the biocatalyst reaction mixture. This allows for the transferral of the reaction mixture minus the magnetic biocatalyst from a first reaction vessel to a second reaction vessel, leaving the magnetic biocatalyst in the first reaction vessel. A second biocatalytic reaction is conducted completely independent of die first biocatalytic reaction, by adding a second biocatalyst immobilized to magnetic particles to the second reaction vessel containing die biocatalytic reaction mixture transferred from the first reaction vessel. Finally, these steps are repeated to accomplish a sequential series of distinct and independent biocatalytic reactions, producing a corresponding series of modified starting compounds. The biocatalytic reactions can also be performed using biocatalysts immobiUzed on any surface which provides for the convenient addition and removal of biocatalyst from the biocatalytic reaction mixture thus accomplishing a sequential series of distinct and independent biocatalytic reactions producing a series of modified starting compounds. The biocatalytic reactions can also be used to derivatize known drug compounds producing new derivatives of the drug compound and select individual compounds witiiin this library that exhibit optimal activity. This is accomplished by die integration of a high affinity receptor into the biocatalytic reaction mixture, which is possible because of the compatibility of the reaction conditions used in biosynthesis and screening. The high affinity receptor is added to the reaction mixture at approximately one half the molar concentration of the starting active compound, resulting in essentially all of the receptor being bound with the starting active compound and an equal molar concentration of starting active compound free in solution and available for biocatalytic modification. If the biocatalytic reaction mixture produces a derivative which possesses a higher binding affinity for the receptor, which can translate into improved pharmacological performance, this derivative will displace die bound starting active compound and remain complexed witii the receptor, and tiius be protected from further biocatalytic conversions. At the end of the experiment, die receptor complex is isolated, dissociated and the bound compound analyzed. This approach accomplishes the identification of an improved version of the drug compound without die need to purify and test each compound individually. The biocatalytic reactions and in vitro screening assays can be performed with the use of an automated robotic device. The automated robotic device having: (a) an XY table with an attached XYZ pipetting boom to add volumetric amounts of enzyme, substrate, cofactor, solvent solutions and assay reagents from reagent vessels positioned on the XY table to reaction and assay vessels positioned on the same XY table; (b) an XYZ reaction-vessel transfer boom attached to the same XY table used to transfer reaction and assay vessels positioned on the XY table to different locations on the XY table; (c) a temperature incubation block attached to the same XY table to house the reaction and assay vessels during reaction incubations and control the temperature of the reaction mixtures; (d) a magnetic separation block attached to die same XY table to separate the biocatalyst immobiUzed to magnetic particles from the biocatalytic mixture by applying an external magnetic field causing the magnetic particles to be attracted to and concentrate at the source of the magnetic filed, thus effectively separating diem from the bulk of the biocatalytic reaction mixture; and (e) a progr-_mmable microprocessor interfaced to the XYZ pipetting boom, and XYZ reaction- vessel transfer boom, the temperature block and die magnetic separation block to precisely control and regulate all movements and operations of these functional units in performing biocatalytic reactions to produce modified starting compounds and assays to determine desired activities. Figure 2 illustrates the automated robotic device of this invention. Mounted in die frame 1 of the system are containers for starting compounds 2, and containers for reagents 2 such as enzymes, cofactors, and buffers. There are specific biosynthesis boxes 4 which contain reagents for various classes of reactions. The frame also has arrays of reaction vessels £, and a heating block 6 with wells 2 for conducting reactions at a specific temperature. The frame has an area £ for reagents for screening test 8 which contains reagents used for conducting screening tests, and area £ which contains assay vessels for conducting screening tests, the automated system uses a X-Y-Z pipetting and vessel transfer boom 10 to dispense aϋ reagents and solutions, and transfer reaction vessels. In operation the X-Y-Z reaction-vessels transfer boom can deliver starting compounds and reagents to specific locations for making specific modified starting compounds which in turn can be delivered to specific locations for conducting assays. In this way the process of making modified starting compounds and testing for optimum activity is largely automated. Figures 4a and 4b Ulustrate derivatization of cast__ιosperrnine and mediotrexate. AU of these embodiments utilize the biocatalytic conversions set out in Table II and die assays set out in Table VI. While the invention as described herein is directed to the development of drugs, those skiUed in the biological arts will recognize that the methods of this invention are equally appUcable to other biologically active compounds such as food additives, pesticides, herbicides, and plant and animal growth hormones. TABLE I. Major Functional Groups Available for Biocatalytic Modification* A. Hydroxyl Groups ~ These groups can undergo numerous reactions including oxidation to aldehydes or ketones (1.1), acylation witii ester donors (2.3, 3.1), glycosidic bond formation (2.4, 3.2, 5.3), and etherification (2.1, 3.3). Potential for stereo- and regio- selective synthesis as weU as prochiral specificity. B. Aldehydes and Ketones - These groups can undergo selective reduction to alcohols (1.1). This may then be foUowed by modifications of hydroxyl groups. C. Amino Groups These groups can undergo oxidative deamination (1.4), N-dealkylation (1.5, 1.11), transferred to other compounds (2.6), peptide bond synthesis (3.4, 6,3), and acylation with ester donors (2.3, 3.1). D. Carboxyl Groups ~ These groups can be decarboxylated (1.2, 1.5, 4.1), and esterified (3.1, 3.6). E. Thiol Groups - These groups can undergo reactions similar to hydroxyls, such as thioester formation (2.8, 3.1), thiol oxidation (1.8), and disulfide formation (1.8). F. Aromatic Groups - These groups can hydroxylated (1.11, 1.13, 1.14), and oxidatively cleaved to diacids (1.14). G. Carbohydrate Groups ~ These groups can be transferred to hydroxyls and phenols (2.4, 3.2, 5.3), and to other carbohydrates (2.4). H. Ester and Peptide Groups -- These groups can be hydrolyzed (3.1, 3.4, 3.5, 3.6, 3.9), and transesterified (or interesterified) (3.1, 3.4). I. Sulfate and Phosphate Groups - These groups can be hydrolyzed (3.1, 3.(, transferred to other compounds (2.7, 2.8), and esterified (3.1). J. Halogens - These groups can be oxidatively or hydrolytically removed (1.11, 3.8), and added (1.11). K. Aromatic Amines and Phenols - These groups can be acylated (2.3, 3.1) or oxidatively polymerized (1.10, 1.11, 1.14). *Numbers in parentheses correspond to the EC (Enzyme Commission) categorization of enzymes and enzyme classes. TABLE II. A Representative Subset of Biocatalytic Reactions Which Mav Be Used to Modify Functional Gronns 1. Oxidation of primary and secondary alcohols; Reduction of aldehydes and ketones. Reaction Boxes: Al, Bl, C2, D2 Enzvme Class: 1.1. Dehydrogenases, Dehydtratases, Oxidases Representative Enzymes: Alcohol dehydrogenase Glycerol Dehydrogenase Glycerol-3-Phosphate Dehydrogenase Xylulose Reductase Polyol Dehydrogenase Sorbitol Dehydrogenase Glyoxylate Reductase Lactate Dehydrogenase Glycerate Dehydrogenase β-Hydroxybutyrate Dehydrogenase Malate Dehydrogenase Glucose Dehydrogenase Glucose-6-Phosphate Dehydrogenase 3α- and 3β-Hydroxysteroid Dehydrogenase 3α-, 20β-Hydroxsteroid Dehydrogenase Fucose Dehydrogenase Cytochrome-Dependent Lactate Dehydrogenase Galactose Oxidase Glucose Oxidase Cholesterol Oxidase Alcohol Oxidase Glycolate Oxidase Xanthine Oxidase Fructose Dehydrogenase Cosubstrates/Cofactors: NAD(P)(H) TABLE II (cont.) 2. Acylation of primary and secondary alcohols. Reaction Boxes: A3, B4 Enzvme Classes: 3.1, 3.4, 3.5, 3.6 Representative Enzymes: Esterases, Upases, proteases, sulfatases, phosphatases, acylases, lactamases, nucleases, acyl transferases Esterases Lipases Phospholipase A Acetylesterase Acetyl Cholinesterase Butyryl Cholinesterase Pectinesterase Cholesterol Esterase Glyoxalase II Alkaline Phosphatase Acid Phosphatase A Variety of nucleases Glucose-6-Phosphatase Fructose 1,6-Diphosphatase Ribonuclease Deoxyribonuclease Sulfatase Chondro-4-Sulfatase Chondro-6-Sulfatase Leucine Aminopeptidase Carboxypeptidase A Carboxypeptidase B Carboxypeptidase Y Carboxypeptidase W Prolidase Cathepsin C Chymotrypsin Trypsin Elastase TABLE II (cont.) Subtilisin Papain 5 Pepsin Ficin Bromelain Rennin Proteinase A 10 CoUagenase Urokinase Asparaginase Glutaminase Urease 15 Acylase I Penicillinase Cephalosporinase Creatininase Guanase 20 Adenosine Deaminase Creatine Deaminase Inorganic Pyrophosphatase ATPase Choline Acetyltransferase 25 Carnitine Acetyltransferase Phosphotransacetylase Chloramphenicol Acetyltransferase Transglutaminase γ-Glutamyl Transpeptidase TABLE II (cont.) Cosubstrates/Cofactors: Esters of alkyl, aryl, charged, polar/neutral groups. These acyl donors can be chosen from the class consisting of the foUowing structural formulae: R-O-CO-R' Where R = alkyl, vinyl, isopropenyl haloalkyl, aryl, derivatives of aryl (i.e., nitrophenyl) and R' can be any alkyl or aryl group with or without derivatives. Such derivatives include halogens, charged functional groups (i.e., acids, sulfates, phosphates, amines, etc.), glycols (protected or unprotected), etc. 3. Transglycosylation of primary and secondary alcohols. Reaction Boxes: A10.B10 Enzvme Class: 2.4, 3.2 Representative Enzymes: Phosphorylase a Phosphorylase b Dextransucrase Levansucrase Sucrose Phosphorylase Glycogen Synthase UDP-Glucuronyltransferase Galactosyl Transferase Nucleoside Phosphorylase α- and β-Amylase Amyloglucosidase (Glucoamylase) CeUulase Dextranase Chitinase Pectinase Lysozyme Neuraminidase Xylanase α- and β-Glucosidase TABLE II (cont.) α- and β-Galactosidase cc- and β-Mannosidase Invertase Trahalase β-N-Acetylglucosarninidase γ-Glucuronidase Hyaluronidase β-Xylosidase Hesperidinase PuUulanase α-Fucosidase Agarase Endoglycosidase F NADase Glycopeptidase F Thioglucosidase Cosubstrates/Cofactors: All available sugars and their derivatives. These sugars can be monosaccharides, disaccharides, and oligosaccharides and tiieir derivatives. 4. Etherification of primary and secondary alcohols Reaction Boxes: Al l. Bll Enzvme Classes: 2.1. 3.2 Representative Enzymes: Catechol α-Methyltransferase Aspartate Transcarbamylase Ornithine Transcarbamylase S-Adenosylhomocysteine Hydrolase Cosubstrates/Cofactors : Alcohols or ethers of any chain length. TABLE II (cont.) 5. Acylation of primary and secondary amines. Reaction Boxes: E3, E4 Enzvme Classes: 2.3, 3.1, 3.4, 3.5, 3.6 Representative Enzymes: Choline Acetyltransferase Carnitine Acetyltransferase Phosphotransacetylase Chloramphenicol Acetyltransferase Transglutaminase γ-Glutamyl Transpeptidase Esterases Lipases Phospholipase A Acetylesterase Acetyl Cholinesterase Butyryl Cholinesterase Pectinesterase Cholesterol Esterase Glyoxylase II AlkaUne Phosphatase Acid Phosphatse A Variety of nucleases Glucose-6-Phosphatase Fructose 1,6-Diphosphatase Ribonuclease Deoxyribonuclease Sulfatase Chondro-4-Sulfatase Chondro-6-Sulfatase Leucine Aminopeptidase Carboxypeptidase A Carboxypeptidase B Carboxypeptidase Y Carboxypeptidase W TABLE II (cont.) Prolidase Cathepsin C Chymotrypsin Trypsin Elastase Subtilisin Papain Pepsin Ficin Bromelin Rennin Proteinase A CoUagenase Urokinase Asparaginase Glutaminase Urease Acylase I Pinicillinase Cephalosporinase Creatininase Guanase Adenosine Deaminase Creatine Deaminase Inorganic Pyrophosphatase ATPase Cosubstrates/Cofactors: See example number 2, above. 6. Esterification of carboxylic acids. Reaction Boxes: 17. Enzvme Classes: 3.1, 3.6 TABLE II (cont.) Representative Enzymes: Esterases Lipases Phospholipase A Acetylesterase Acetyl Cholinesterase Butyryl Cholinesterase Pectinesterase Cholesterol Esterase Glyoxylase II AlkaUne Phosphatase Acid Phosphatase A Variety of nucleases Glucose-6-Phosphatase Fructose 1,6-Diphosphatase Ribonuclease Deoxyribonuclease Sulfatase Chondro-4-Sulfatase Chondro-6-Sulfatase Inorganic Pyrophosphatase APTase Cosubstrates/Cofactors: alcohols of any chain length being alkyl, aryl, or their structural derivatives. TABLE III. Biocatalytic "Reaction Box" Matrix Approach TABLE III (cont. 1) Biocatalytic "Reaction Box" Matrix Approach TABLE III (cont. 2) Biocatalytic "Reaction Box" Matrix Approach TABLE III (cont. 3) Biocatalytic "Reaction Box" Matrix Approach TABLE IV. Reaction Box Analysis of AZT Derivatization Indicating the Total Number of Possible Reaction Products (a) Assuming 30 different acyl donors to be added to this reaction mixture. This includes alkyl, aryl, and of different lengths, (b) Assuming 30 UDP-sugars used in tiiis reactin box, (c) Reduction of the ketones to secondary alcohols leads to die potential acylation of the secondary alcohols and adds 30-fold more potential products; and (d) Each box's possible permutations are multiplied together to estimate the total number of compounds syntiiesized. TABLE V. Reaction Box Analysis of Established Drugs Indicating the Total Number of Possible Reaction Products Number of Estimated Number Starting Compound Functional Groups of Derivatives Castanospeimine 4 810,000 Cyclosporin 24 billions Gentamicin 8 billions Haloperidol 3 120 Methotrexate 7 greater than 10* Muscarine 2 2,400 Prazosin 6 288,000 Prednisone 12 46,080,000 Thyroxine 3 2,160,000 Valproic Acid 1 900 Vancomycin many billion TABLE VI. Representative Subset of Screening Assays Possible to Test for Anti-Cancer. Anti-Viral and Anti-Hvpertensive Activities Anti-Cancer Drugs 1. KB (Eagle) cell culture assay 2. Inhibition of the growth of human breast cancer cell lines in vitro 3. Inhibition of the growth of P388 leukemia cells in vitro 4. Inhibition of the growth of murine L 1210 cells in vitro 5. Inhibition of gylcinamide ribonucleotide formyltransferase activity 6. Inhibition of ribonucleotide reductase activity 7. Inhibition of protein kinase C activity 8. Inhibition of human aromatase activity 9. Inhibition of DNA topoisomerase II activity 10. Inhibition of dihydrof olate reductase 11. Inhibition of aminoimidazole carboxamide ribonucleotide formyltransferase Anti-AIDS Drugs 1. Inhibition of HIV virus replication devoid of cytotoxic activity 2. Inhibition of HIV protease activity 3. Soluble-formazan assay for HIV- 1 4. Inhibition of HTV reverse transcriptase activity Anti-Hypertensive Drugs 1. Inhibition of ACE activity 2. Inhibition of human plasma renin 3. Inhibition of in vitro human renin 4. Inhibition of angiotensin converting enzyme 5. Alpha 1-adrenergic receptor binding assay 6. Alpha 2-adrenergic receptor binding assay 7. Beta-adrenergic receptor binding assay (bronchodilator, cardiotonic, tocolytic, anti- anginal, anti-arrhythmic, anti-glaucoma) 8. Dopamine receptor binding assay (anti-migraine, anti-parkinsonian, anti-emetic, anti- psychotic) Example 1 1. Enzymatic esterification of taxol 1.1 Analysis of reaction products Products of enzymatic esterification of taxol were analyzed using reversed-phase HPLC on a Waters system with a 990 photodiode array detector, and a 3.9 x 300 mm μBondapak Cjg column. The solvent system used consisted of a wateπacetonitrile mixture (40:15 v/v) and isopropanol. Elution was performed at 1 ml/min according to the following linear gradient program: Product peaks were detected at 227 nm using a photodiode array detector. 1.2 Enzvmatic synthesis of taxol esters - Identification of active biocatalysts fReaction Box B-4^ A mixture of 55 enzymes (Table VH) consisting of crude Upases, crude proteases, and purified proteases (total mass of 600 mg) was added to a solution of taxol (17.0 mg, 20 μmol) and vinyl butyrate (0.25 ml, 1.5 mmol) in 2.9 ml tert amyl alcohol. The mixture was placed into a septum-sealed glass vial, sonicated for 30 s, and put into an orbit shaker operating at 250 rpm and 35°C for a period of 48 h. The reaction was tiien stopped by removing the suspended solid catalyst by centrifugation. The supernatant containing taxol esters was evaporated to dryness in vacuum and redissolved in 0.3 ml methanol. An aliquot of the resultant concentrated solution (15 μl) was diluted with 250 μl methanol and analyzed by HPLC as described in 1.1. The chromatogram revealed a peak with a retention time of 9.7 min (unreacted taxol), and a peak at 14.6 min (2 -taxol butyrate, yield 7%). The enzymes were then spUt into three groups: 26 pases (100 mg/ml), 26 crude proteases (100 mg/ml), and 4 purified proteases (10 mg/ml) and the identical reaction as above performed. Only the purified proteases showed significant activity with a yield of 2'-butyrate of 15% after 48 h. The purified proteases were then divided into individual enzymes (all used at a concentration of 10 mg/ml) and thermolysin (a bacterial protease from Bacillus thermoproteolyticus rokko a.k.a. thermolysin) was identified as the most active enzyme with a yield after 48 h of 24%. This approach demonstrates that active biocatalysts can be easily identified by a sequential process of eUminating unreactive biocatalysts. TABLE VII. Enzymes used in the acylation of taxol Lioases and Esterases Enzvme Source Company CES Amano L-10 Amano G Penicillium sp. Amano N Rhizopus niveus Amano AP Aspergillus niger (acid stable) Sigma Wheat Germ Amano GC-20 Geotrichwn candidum Amano AY-30 Candida rugosa Amano P Pseudomonas cepacia Amano AG-975 Sigma Porcine Pancreatic Amano APF A. niger Amano R-10 Amano AK Pseudomonas sp. Amano PGE Calf tongue root Amano D Amano GC-4 Amano CE Sigma Candida rugosa Amano FAP-15 Rhizopus sp. Amano MAP- 10 Mucor sp. Amano Enzeco K16825 Enzeco Lamb Pre-Gastric Esterase Quest Calf Pre-Gastric Esterase Quest Esterase 30,000 #3586 Gist Brocades Lipase 80,000 #5093 Rhizopus sp. Gist Brocades TABLE VII. (cont.) Enzymes used in the acylation of taxol IL Proteases Enzvme Source Company B Penicillium sp. Amano Papain Papaya Sigma 2A Aspergillus oryzae Amano Proleather Bacillus sp. Amano N B. subtilis (neutral) Amano Acid Stable Rhizopus sp. Amano Alcalase-2T B. Licheniformis (Subtilisin Carlsberg) Novo Bromelain Pineapple Sigma Alkaline Protease Quest Fungal Protease #9240810 Biocon Acid Protease #8221108 Biocon Neutral Protease 900,000 Biocon #6W16B HT MKC Rapidase S-90 B. subtilis Gist Brocades HT-Proteolytic 200 Solvay Opticlean M-375 Alkaline protease (from Bacillus sp). Solvay Optimase M-440 Alkaline protease (from Bacillus sp). Solvay Fungal Protease 60,000 Solvay Esperase-4T Alkaline protease (from Bacillus sp). Novo Peptidase A A. oryzae Amano Prozyme 6 Aspergillus sp. Amano Protease M A. oryzae Amano Newlase A A. niger (acid stable) Amano Acylase Cone. Aspergillus sp. Amano Protease S Bacillus sp. (thermostable) Amano Acylase I Porcine kidney Sigma III. Purified Enzvmes Enzvme Source Company Trypsin Bovine pancreas Sigma Chymotrypsin Bovine pancreas Sigma Subtilisin Carlsberg Bacillus liquefaciens Sigma Protease X B. thermoproteolyticus rokko Sigma 1.3. Enzymatic synthesis of a mixture of taxol esters - Sequential reaction box derivatization of taxol The optimal enzyme catalyst used for the synthesis was produced by freeze-drying an aqueous solution containing thermolysin, KC1 and potassium phosphate buffer adjusted to pH 7.5. The solid catalyst obtained after free-drying contained 5% enzyme, 94% KC1 and 1% potassium phosphate. The powdered enzyme catalyst (335 mg - containing 16.75 mg thermolysin) was added to a solution of taxol (17.0 mg, 20 μmol) and vinyl caproate (straight- chain C _ * ester) (0.24 ml. 1.5 mmol) in 2.9 ml tert-amyl alcohol. The mixture was placed into a septum-sealed glass vial, sonicated for 30 s, and put into an orbit shaker operating at 250 rpm and 35°C. After 28 h the reaction mixture (including enzyme) was removed and the fuU contents of the mixture added to a separate solution containing vinyl propionate (0.24 ml, 2.2 mmol), vinyl acrylate (0.24 ml, 2.2 mmol), and vinyl butyrate (0.24 ml, 1.9 mmol). This second reaction was placed on die shaker and incubated at 250 rpm at 35°C. After 24 h, the reaction mixture (including enzyme) was removed and die full contents of the mixture added to a separate solution containing vinyl acetate (0.25 ml, 2.6 mmol) and vinyl chloroacetate (0.24 ml, 1.8 mmol). This reaction was allowed to proceed for 24 h at 250 rpm and 35°C. The sequential reaction was tiien stopped by removing the suspended soUd catalyst by centrifugation. The sequential reaction was aided by the soluble nature of the taxol and taxol derivatives. The supernatant containing taxol esters was evaporated to dryness in vacuum and redissolved in 0.3 ml methanol. An atiquot of the resultant concentrated solution (15 μl) was diluted witii 250 μl methanol and analyzed by HPLC as described in 1.1. The chromatogram revealed a peak with retention time 9.7 min (unreacted taxol), a broad peak between 11 and 13 min (total estimated yield 52%), peaks at 14.6 min (yield 9%) and 17.4 min (yield 18%), and a small peak at 18.4 min (yield 0.2%). Total reaction yield was approximately 80%. 1.4 Removal of taxol from reaction mixture A concentrated methanol solution of reaction products produced as described in 1.3 was appUed on a preparative TLC silica plate (Whatman, 20x20 cm, siUca layer thickness 500 μm, containing fluorescent marker), and die plates were developed using a solvent mixture of chloroform:acetonitrile (4: 1 v/v). Positions of product spots were determined by irradiating the plates with ultraviolet Ught. The Rf value of taxol is 0.16 and the Rf of the taxol esters range from 0.28 to 0.71. The products were removed from the TLC plate and dissolved in ethyl acetate. The products were then dried in vacuo. 1.5 Screening the mixture of taxol esters The library of taxol derivatives described above was screened for cytotoxicity against HL- 60 ceUs, a promyelocytic leukemia cell line, and MOLT-4 ceUs, a lymphoblastic leukemia ceU line. Cells were seeded in 96-well plates at densities of 30,000 ceUs/well and grown in RPMI-1640 medium containing 10% bovine fetal calf serum at 37 °C for 24 h. The medium was tiien replaced with fresh medium containing the taxol derivatives (excluding taxol, which had been removed by preparative tiiin-layer chromatography) dissolved in DMSO at final concentrations ranging from 100 nM to 0.1 nM. The final concentration of DMSO in the cell medium was 0.5% (v/v ). After 72 h, samples were removed for cell counts. Total ceU number and viabiUty were determined by trypan blue exclusion and manual ceU counting on a hemacytometer. The dose-response data are reported in Table IX. The hemacytometer ceU counts revealed that the taxol-derivative Ubrary contained at least one cytotoxic derivative. 1.6 Backtracking to identify active produces ' ) 1.6.1 Enzymatic synthesis of individual taxol esters - Identification of active taxol esters by building-up the reactions comprising taxol acylation in 1.3 above. Given the predictable nature of the enzymatic acylation reactions, it is possible to identify the possible products without analyzing the chemical compositions of the mixture. The possible products were, therefore, synthesized individuaUy, as described below, and screens on these individual ester products were tiien initiated as part of the backtracking process. The powdered enzyme catalyst prepared as described in 1.3 above (140 mg) was added to a solution of taxol (5.5 mg, 6.5 μmol) and on individual vinyl ester (80 μl, approximately 2 mmol) in 1.0 ml tert-amyl alcohol. The following vinyl esters were used as acylating agents: acetate, chloroacetate, acrylate, propionate, butyrate, and caproate. Each mixture was placed into a septum-sealed glass vial, sonicated for 30 s, and put into an orbit shaker operating at 250 rpm and 35°C. After 96 h the reaction was stopped by removing the suspended solid catalyst by centrifugation. Supematants containing taxol esters were evaporated to dryness in vacuo and redissolved in 0.3 ml methanol each. An aliquot of each resultant concentrated solutions (20 μl) was diluted with 80 μl methanol and analyzed by HPLC as described in 1.1. Results of HPLC analysis are given in Table VHI. Table VIII. Retention times (RT) and Rf values of Taxol esterification products* *Taxol: retention time 9.7 min. Rf 0.16. 1.6.2. Recovery of reaction products bv tiiin layer chromatography fTLCD Individual ester reaction products were separated from taxol by TLC. Concentrated methanol solutions of reaction products produced as described in 1.6 were appUed on preparative TLC siUca plates (Whatman, 20x20 cm, sUica layer thickness 500 μm, containing fluorescent market), and the plates were developed using a solvent mixture of chloroform:acetonitrile (4:1 v/v). Positions of product spots were determined by irradiating the plates with ultraviolet Ught. The Rf values of the products are given in Table VIII. Product spots were scraped off the plates separately and scrapings were eluted witii 15 ml ethyl acetate to recover the product Dry products were obtained by evaporation of ethyl acetate in vacuo. 1.6.3 Screening individual taxol esters To determine which of the newly synthesized product(s) was active, the synthetic history of the library was "backtracked" by enzymaticaUy synthesizing aU possible ester products individuaUy (as described above). Following isolation of the products by TLC, each derivative was tested for cytotoxic activity as described above. The cytotoxicity experiments revealed that two derivatives, 2'-chloroacetyltaxol and 2'-acryloyltaxol, are active against both ceU lines (Table IX). Table IX. Percent Viability of MOLT-4 and HL-60 Cells After 72 h exposure to taxol and its derivatives at various concentrations 100 nM 10 nM I nM 0.1 nM MOLT-4 Taxol 1.22 11.1 92.8 92.9 Library 3.13 67.9 95.9 95.0 2 ' -Chloroacetyltaxol 4.26 91.9 94.4 96.9 2'-Acryloyltaxol 2.70 61.0 95.6 91.0 HL-60 Taxol 0.00 32.6 96.1 98.3 Library 5.26 98.3 98.5 99.0 2 ' -Chloroacetyltaxol 2.13 99.5 97.3 99.5 2'-Acryloyltaxol 4.69 87.1 96.9 97.5 ■ Example 2 2. Enzymatic hydrolysis of taxol 2.1 Analysis of reaction products Products of enzymatic hydrolysis of taxol were analyzed using reversed-phase HPLC on a Waters system widi a 990 photodiode array detector, witii 3.9 x 300 mm μBondapak Cj column. The solvent system used consisted of water and acetonitrile. The solvents were fed into the HPLC system at 1 ml min according to die foUowing Unear gradient program: Time (min) Solvent Composition (v/v %) Acetonitrile Water 0-1 25 75 1-5 45 55 5-26 58 42 26-30 100 0 30-35 25 75 Product peaks were detected at 227 nm using a photodiode array detector. 2.2 Enzymatic hydrolysis of taxol (Reaction Boxes J-8 and S-8) The enzyme catalyst used for hydrolysis was produced by freeze-drying an aqueous solution (adjusted to pH 7.5) containing equal weight amounts of thermolysin, subtiUsin Carlsberg, chymotrypsin and trypsin. The enzyme catalyst (1.1 mg) was dissolved in 0.7 ml 0.1 M potassium phosphate buffer pH 7.5. The aqueous enzyme solution was added to a solution of taxol (2.0 mg, 2.4 μmol) in 0.3 ml tert amyl alcohol. The biphasic reaction system produced in this way was placed into a septum-sealed glass vial and put on an orbit shaker operating at 75 strokes/min and 20°C. After 26 h the organic solvent layer was separated, evaporated to dryness and redissolved in 0.2 ml methanol for HPLC analysis. The aqueous phase was diluted witii 3.5 ml methanol and insoluble solids (precipitated enzymes) were removed by centrifugation. Clear supematant was evaporated into dryness and redissolved in 0.2 ml methanol for HPLC analysis. HPLC analysis was performed as described in 2.1. Neither taxol, nor any hydrolysis products were detected in the methanol solution obtained after workup of the aqueous phase of the biphasic reaction system. On the other hand, the chromatogram of the methanol solution of the dry residue obtained from the organic layer revealed a peak with retention time of 20.4 min (unreacted taxol) and a peak at 18.4 min, representing a product of enzymatic hydrolysis of taxol, as identified by the characteristic uv-absorbance scan. The estimated yield of this product was 9%. Example 3 3. Enzymatic glycosylation of taxol 3.1 Analysis of Reaction Products Products of enzymatic glycosylation of taxol were analyzed using reversed-phase HPLC on a Waters system with a 990 photodiode detector, with a 3.9 x 300 mm μBondapak Ci column. The solvent system used consisted of water and methanol. The solvents were fed into the HPLC system at 1 ml/min according to the foUowing Unear gradient program: Time (min) Solvent Composition (v/v %) Methanol Water 0-5 25 75 5-37 85 15 37-40 100 0 40-42 100 0 42-44 25 75 Product peaks were detected at 227 nm using a photodiode array detector. 3.2 Enzymatic glvcosylation of taxol Glycosylation of taxol was performed using α-glucosidase from baker's yeast as a biocatalyst For reference, the enzymes used were: α-glucosidase from brewer's yeast (pH 7.0), α-glucosidase from baker's yeast (pH 7.0), β-galactosidase from E. coli (pH 5.0), β- galactosidase from A. oryzae (pH 5.0), β-glucuronidase from bovine liver (pH 5.0). These enzymes were used in two mixtures (one at pH 7.0 and one at pH 5.0) and it was determined tiiat only the mixture at pH 7.0 was active in glycosylating taxol. This mixture was then divided into individual enzymes where it was found that α-glucosidase from baker's yeast was the active biocatalyst.

Claims: WHAT IS CLAIMED IS: 1. A method for drug identification comprising: (a) conducting a series of biocatalytic reactions by mixing biocatalysts with a starting compound to produce a reaction mixture and tiiereafter a library of modified starting compounds; (b) testing the library of modified starting compounds to determine if a modified starting compound is present within the Ubrary which exhibits a desired activity; (c) identifying the specific biocatalytic reactions which produce the modified starting compound of desired activity by systematically eUminating each of the biocatalytic reactions used to produce a portion of the Ubrary, and then testing the compounds produced in die portion of the library for the presence or absence of the modified starting compound with the desired activity; and (d) repeating the specific biocatalytic reactions which produce die modified compound of desired activity and determining die chemical composition of the reaction product. 2. A method for drug identification of Claim 1 wherein (a) the biocatalytic reactions are conducted with a group of biocatalysts that react with distinct structural moieties found witiiin the structure of a starting compound, (b) each biocatalyst is specific for one structural moiety or a group of related structural moieties; and (c) each biocatalyst reacts with many different starting compounds which contain the distinct structural moiety. 3. A method for drug identification comprising: (a) conducting a series of biocatalytic reactions on a starting compound to produce a Ubrary of modified starting compounds; (b) providing a high affinity receptor for a starting compound of desired activity and exposing the modified compounds formed after each biocatalytic reaction to die high affinity receptor, (c) isolating the high affinity receptor-modified starting compound complex from die biocatalytic reaction mixture; (d) dissociating die complex into its component parts; and (e) determining the chemical composition of the dissociated modified starting compound. 4. A method for drug identification comprising: (a) adding a high affinity receptor to a starting compound to form a biocatalytic reaction mixture at a concentration that allows for essentially aU of the high affinity receptor to bind with the starting compound and about an equal molar amount of starting compound remaining free in solution and available for biocatalytic modification; (b) producing a modified starring compound exhibiting a higher binding affinity than the starting compound resulting in the displacement of die starting compound from the high affinity receptor; (c) forming a complex of the modified starting compound and die high affinity receptor, thereby protecting the modified starting compound from further biocatalytic reaction; (d) isolating the complex from the biocatalytic reaction mixture; (e) dissociating die complex into its component parts; (f) determining the chemical composition of die dissociated modified starting compound; and (g) repeating steps (a) through (b) to produce a Ubrary of modified starting compounds. 5. A method for drug identification according to Claim 4 wherein the high affinity receptor is present on the surface or contained widiin a Uving ceU or immobiUzed to a natural or artificial support. 6. A method for drug identification according to Claim 1 wherein the biocatalytic reactions are selected from a group consisting of: (a) Oxidation of primary and secondary alcohols; (b) Reduction of aldehydes and ketones; (c) Acylation of primary and secondary alcohols; (d) Transglycosylation of primary and secondary alcohols; (e) Etherification of primary and secondary alcohols; (f) Acylation of primary and secondary amines; and (g) Esterification of carboxylic acids. 7. A metiiod for drug identification according to Claim 1 wherein the biocatalyst corhprises crude or purified enzymes, ceUular lysate preparations, partiaUy purified lysate preparations, living ceUs and intact non-Uving cells, used in a soluble, suspended or immobUized form. 8. A method for drug identification according to Claim 1 wherein the biocatalytic reactions are used in combination witii non-specific chemical reactions to produce a Ubrary of modified starting compounds. 9. A method for drug identification according to Claim 1 further comprising: (a) exposing the reaction mixture to a drug screening device tiiat measures the binding of compounds with desired activity to locaUzed or immobilized receptor molecules or cells; (b) correlating a positive measurement from the drug screening device with die sequence of biocatalytic reactions used to syndiesize the reaction mixture and die specific reaction sequence producing the modified starting compound with desired activity; and (c) repeating the biocatalytic reaction sequence to produce die modified starting compound of desired activity and to determine its chemical composition. 10. A method for drug identification according to Claim 1 wherein the biocatalytic reaction is performed witii a biocatalyst immobilized to magnetic particles forming a magnetic biocatalyst, the method further comprising (a) initiating the biocatalytic reaction by combining the immobilized biocatalyst with substrate(s), cofactor(s) and solvent/buffer conditions used for a specific biocatalytic reaction; (b) removing the magnetic biocatalyst from the biocatalytic reaction mixture to terminate the biocatalytic reaction, which is accompUshed by applying an external magnetic field causing the magnetic particles witii the immobilized biocatalyst to be attracted to and concentrate at the source of die magnetic field, thus effectively separating the magnetic biocatalyst from the bulk of the biocatalytic reaction mixture and aUowing for the transferral of the reaction mixture minus the magnetic biocatalyst from a first reaction vessel to a second reaction vessel, leaving the magnetic biocatalyst in the first reaction vessel; (c) conducting a second biocatalytic reaction, completely independent of die first biocatalytic reaction, by combining a second biocatalyst immobilized to magnetic particles with the second reaction vessel containing the biocatalytic reaction mixture transferred from the first reaction vessel; and (d) repeating steps a) through c) to accompUsh a sequential series of distinct and independent biocatalytic reactions and produce a corresponding series of modified starting compounds. 11. A method for drug identification according to Claim 10 wherein the biocatalytic reaction is performed with a biocatalyst immobUized to a particle and the biocatalyst is removed from the biocatalytic reaction mixture by centrifugation or filtration. 12. A method for drug identification according to Claim 1 using an automated robotic device, the automated robotic device comprised of: (a) an XY table with an attached XYZ pipetting boom to add volumetric amounts of enzyme, substrate, cofactor, solvent solutions and assay reagents from reagent vessels positioned on the XY table to reaction and assay vessels positioned on die same XY table; (b) an XYZ reaction-vessel transfer boom attached to die same XY table used to transfer reaction and assay vessels positioned on the XY table to different locations on die XY table; (c) a temperature incubation block attached to die same XY table to house the reaction and assay vessels during reaction incubations and control the temperature of the reaction mixtures; (d) a magnetic separation block attached to the same XY table to separate the biocatalyst immobilized to magnetic particles from the biocatalytic mixture by applying an external magnetic field causing the magnetic particles to be attracted to and concentrate at the source of the magnetic filed, tiius effectively separating them from the bulk of the biocatalytic reaction mixture; and (e) a programmable microprocessor interfaced to the XYZ pipetting boom, and XYZ reaction vessel transfer boom, the temperature block and the magnetic separation block to precisely control and regulate aU movements and operations of these functional units in performing biocatalytic reactions to produce modified starting compounds and assays to determined desired activities. 13. A method for rapid drug development comprising: (a) mixing a starting drug compound with a high affinity receptor at approximately one half the molar concentration of die starting drug compound to aUow essentially aU of the high affinity receptor to bind to the starting drug compound and leave an equal molar amount of starting drug compound free in solution and avadable for biocatalytic modification; (b) producing a modified drug compound from the biocatalytic reaction between the starting drug compound and the receptor, the modified drug compound having a higher binding affinity than the starting drug compound; (c) displacing the starting drug compound from the receptor witii the modified drug compound; (d) protecting die bound modified drug compound from further biocatalytic reactions; (e) dissociating the complex of the modified drug compound and receptor into its component parts; (f) determining the chemical composition of the dissociated modified starting drug compound; and (g) repeating steps a) dirough e) and tiius conduct a series of biocatalytic reactions on a starting drug compound to produce a Ubrary of modified drug compounds with higher binding affinities. 14. A method for rapid drug development according to Claim 1 wherein the high affinity receptor is present on the surface or contained within a Uving ceU or immobUized to a natural or artificial support. 15. A method for rapid drug development according to Claim 1 wherein the biocatalytic reactions are selected from a group consisting of tiiose listed in Table II. 16. A method for rapid drug development according to claim 1 wherein the testing of the library of modified starting compounds is selected from a group of assays consisting of those listed in Table VI. 17. A method for rapid drug development according to claim 1 wherein the biocatalytic reaction is performed witii a biocatalyst immobiUzed to magnetic particles forming a magnetic biocatalyst whereas: (a) the biocatalytic reaction is initiated by combining the immobilized biocatalyst with the foUowing to form the biocatalytic reaction mixture: substrate(s), cofactor(s) and solvent/buffer conditions used for the specific biocatalytic reaction; (b) the biocatalytic reaction is terminated by removing the magnetic biocatalyst from the biocatalytic reaction mixture, which is accompUshed by applying an external magnetic field causing the magnetic particles with the immobUized biocatalyst to be attracted to and concentrate at the source of the magnetic field, tiius effectively separating the magnetic biocatalyst from the bulk of the biocatalytic reaction mixture and aUowing for the removal of the reaction mixture minus the magnetic biocatalyst to a second reaction vessel, leaving the magnetic biocatalyst in the first reaction vessel; (c) a second biocatalytic reaction, completely independent of the first biocatalytic reaction, is initiated by adding a second biocatalyst immobiUzed to magnetic particles to the second reaction vessel containing the biocatalytic reaction mixture from the first biocatalytic reaction minus the magnetic biocatalyst used in the first biocatalytic reaction; (d) die above described biocatalytic reaction cycle is repeated tiius accomptishing a series of distinct and independent biocatalytic reactions producing a corresponding series of modified drug compounds. (e) a magnetic separation block attached to die same XY table as above to separate the biocatalyst immobiUzed to magnetic particles from the biocatalytic reaction mixture by applying an external magnetic field causing the magnetic particles to be attracted to and concentrate at the source of the magnetic field, tiius effectively separating d em from the bulk of the biocatalytic reaction mixture; and (f) a programmable microprocessor interfaced to die XYZ pipetting boom, the XYZ reaction vessel transfer boom, the temperature block and die magnetic separation block to precisely control and regulate aU movements and operations of these functional units in performing biocatalytic reactions to produce modified starting compounds and assays to determined desired activities. 18. A method according to Claims 1, 3, 4 and 13 wherein the starting compounds are soluble in the reaction mixture. 19. A method according to Claim 18 wherein the starting compounds and resulting derivatives are soluble, and remain soluble for use in screening procedures. 20. A mediod according to Claims 1 wherein identifying the specific biocatalytic reactions which produce the modified starting compound of desired activity is done by systematicaUy budding up each of die biocatalytic reactions used to produce a portion of the library.

Inventors: CLARK DOUGLAS S, DORDICK JONATHAN S, FOX J WESLEY, CLARK, DOUGLAS, S., DORDICK, JONATHAN, S., FOX, J., WESLEY
Applicants: CLARK DOUGLAS S, DORDICK JONATHAN S, ENZYMED INC, FOX J WESLEY, CLARK, DOUGLAS, S., DORDICK, JONATHAN, S., ENZYMED, INC., FOX, J., WESLEY
Application year: 1994
Main classification: C12P1
IPC codes: B01J19, C07K1, C12P1, G01N33, G01N35