A METHOD TO PRODUCE A SUBSTANCE TO INHIBIT CELL PENETRATION, AND A CONTRACEPTIVE TO PREVENT CONCEPTION, AND A THROMBUS, PROSTATE AND AIDS VACCINE AND MEDICINE

Abstract: An inhibitor preventing penetration of the cell membrane is produced as follows: A reactant, which consists of a spermatozoon or the SP-10 molecule, known in itself, found at the end of a spermatozoon, and/or of protein PH-20 or equivalent, such as a synthetically produced substance, is brought into contact with a second reactant, which is a substance found on the surface of the ovum and/or involved in the zona reaction of the ovum or an equivalent substance, such as a synthetically produced substance, the combination of said reactants leading to the formation of a substance inhibiting penetration of the cell membrane. The inhibitor preventing penetration of the cell membrane may be implemented as a contraceptive preventing fertilization, such as a contraceptive liquid for temporary use and/or a contraceptive vaccine and/or a contraceptive pill, e.g. for use by men as well as women. It may contain an antibody of the SP-10 molecule and an antibody of the PH-20 protein and guanosine triphosphate. An inhibitor preventing penetration of the cell membrane of red blood cells and blood platelet cells may be implemented as a liquid or equivalent, e.g. as a pill, for use as a thrombosis vaccine and/or medicine. An inhibitor preventing penetration of the cell membrane of white blood cells may be implemented as a liquid or equivalent, e.g. a pill, for use as an AIDS vaccine or medicine. The above-mentioned inhibitor, vaccines and medicines can be studied and developed e.g. by means of a blood-semen pretesting device.

Description: A METHOD TO PRODUCE A SUBSTANCE TO INHIBIT CELL PENETRATION, AND A CONTRA CEPTIVE TO PREVENT CONCEPTION. AND A THROMBUS. PROSTATE AND AIDS VACCINE AND MEDICINE The present invention relates to a method for producing a substance inhibiting penetration of the cell membrane, to a contraceptive and to a vaccine and medicine against thrombosis, prostatitis and AIDS. In the zona reaction in the ovum, a safe and effective substance was unexpectedly found which can be used as a contraceptive both for males and females and which can also be used to prevent adherence between blood platelets and e. g. to prevent thrombosis and prostatitis and prostate tumour in middle-aged men, and also to inhibit penetration of the cell membrane by the HIV virus. E. g. in a pretesting device as presented in utility design FI NO. 473, when there are egg cells on the contact plate and a spermatozoon penetrates through the cell membrane of an ovum in the device or when this happens in nature, the known zona reaction takes place in the ovum and the ovum releases a substance which forms on the egg cells and also on blood corpuscles an envelope which prevents the cell membrane destroying substance present at the end of sperm cells from penetrating through the cell membrane of egg cells and blood corpuscles. In addition to preventing fertilization, the inhibitor also prevents in blood-semen mixture the generation of a reaction corresponding to a thrombosis aggregation and guards the cell membrane of blood platelets and red blood cells against penetration by the substance causing thrombosis, in other words, it forms the basic element of a preventive medicine against thrombosis. In addition, the inhibitor prevents the SP-10 molecule and corresponding substances from penetrating into the cells of the prostate gland and thus forms a protection against prostatitis and prostate tumour. The inhibitor, used alone or together with certain antibodies, also inhibits the penetration of the cell membrane of leucocytes by the HIV virus, i. e. it constitutes the basic element of an AIDS vaccine and medicine. The device presented in the above-mentioned utility design for the preliminary testing of thrombosis, AIDS and contraceptive vaccines uses a blood-semen mixture in a ratio of about 4: 1 and a propeller wheel or an air blast nozzle which mixes and slings the blood-semen mixture onto the contact plate. The blood cells, sperm cells and egg cells live and remain active in the mixture during the testing, which takes about half an hour. The pretesting device allows relatively quick and safe testing outside the human system e. g. of the ability of vaccines and medicines to prevent the penetration of the cell membrane of egg and blood cells by detrimental substances. Fertilization inhibitor: It is known that the chemical substance formed at the end of spermatozoa, called SP-10 molecule, destroys the cell membrane of the ovum, permitting the spermatozoon to penetrate the cell membrane of the ovum. When this occurs, a zona reaction as mentioned above takes place, in which a substance is released around the ovum which prevents double fertilization, i. e. prevents penetration of the cell membrane by other SP-10 molecules and spermatozoa. Example: According to an article in issue 3/1989 of the Finnish periodical Tiede-2000 (Science), scientists at the University of Connecticut produced a contraceptive vaccine that worked with both male and female guinea pigs by inoculating the guinea pigs with a substance called protein PH-20, found on the surface of a guinea pig's sperm cells. PH-20 somehow produced in the guinea pigs an antibody which prevented the acitivity of sperm cells during a period of about three months. The mechanism of the generation of the PH-20 antibody in guinea pigs is not known. A corresponding vaccine for humans would obviously be safer and more reliable than the currently used, hormone based contraceptive pills. However, no method for producing a corresponding fertilization inhibitor for humans is known. Example: The SP-10 molecule mentioned above, which is formed at the end of the human sperm cell and which penetrates through the cell membrane of an ovum, is examined in issue 3/92 of the Finnish publication Tieteen Kuvalehti (Science Magazine). This publication describes an animal test with a preventive vaccine in which SP-10 molecules were injected into a sow. The animal developed an antibody that prevented fertilization. However, producing an SP-10 antibody with this technique is difficult, and if SP-10 molecules were used on humans as a contraceptive vaccine, they might cause side effects, such as a risk of thrombosis. Example: According to an article in issue 7/93 of the Finnish periodical Tiede 2000, scientists at a university in Israel have found in their tests on frogs that the ATP solution, known in itself, causes the receptors on the surface of a frog's ovum to secrete sodium ions. The scientists discovered that the sperm cell generates an electric current which helps it penetrate the cell membrane of the ovum and promotes the occurrence of a zona reaction. According to the scientists'observations, the antibody of the ATP molecules, gynocine-triphosphate, locked the receptors of the frog's egg cells, preventing the fertilization of the ovum. Said antibody of the ATP molecule known in itself and producing energy in the metabolism of plants and animals does not work in humans to inhibit the penetration of the cell membrane of an ovum by the spermatozoon and it cannot be used to limit the human birth rate. Previously known are hormone-based contraceptive pills, which prevent fertilization by preventing the release of eggs. Inhibitor of thrombosis, prostatitis and prostate tumour: In certain countries, the average age of men is considerably below that of women. As is known, the barrier between a blood vessel and testicle tissue is very thin and easy to penetrate, so SP-10 molecules are likely to be absorbed into the blood of especially middle-aged men. This substance destroys the cell membrane of blood platelets and renders them more liable to stick together and to the wall of a blood vessel to form a thrombus. Especially in men above middle age, SP-10 molecules or equivalent probably also penetrate into the cells of the prostate, causing undesirable expansion of the prostate gland as well as prostatitis and the formation of a prostate tumour. When the above-mentioned inhibitor is injected e. g. into the prostate, into the seminal vesicle, a muscle or the blood, it inhibits, besides fertilization, the formation of thrombi and the development of prostatitis and prostate tumour. Special issue 16/84 of the Finnish magazine Duodecim discusses e. g. the role of prostaglandins, developed in the prostate glands, in the formation of a thrombus. According to the publication, thromboxan TxA2 promotes the aggregation of blood platelets, while e. g. prostacycline PG12, in a layer e. g. on the surface of a blood vessel, prevents the agglomeration of blood platelets and their forming a clot on the wall of the blood vessel. AIDS vaccine and medicine: For example issue 4/91 of the Finnish publication Tieteen Kuvalehti (Science Magazine) describes how a molecule called CD4 together with a molecule called gp 120 and the HIV virus penetrates through the cell membrane of a white corpuscle and primarily that of a T-cell, causing cell destruction and AIDS. E. g. according to a program shown on the Finnish TV on 26 Nov. 1993, scientists at the Pasteur Institute in France have found on the surface of an HIV-infected molecule a substance that together with the CD4 molecule plays a role in the penetration of the cell membrane. This substance is designated CD 26 molecule. In this mechanism, enzymes released from the CD 26 cell break the sheath of the AIDS virus. It has not been possible to destroy the HIV virus, but the outbreak of AIDS can be completely hindered by preventing the penetration of the cell membrane of white corpuscles by the HIV virus. Humans can be given a T-cell penetration inhibitor in the form of AIDS vaccine, and it also acts as a medicine retarding the advance of the disease of a HIV-infected person. An AIDS vaccine and medicine can be safely and relatively quickly researched, developed and pretested outside the human system by using a blood-semen pretesting device as mentioned above. The object of the present invention is to achieve a method to produce a substance inhibiting the penetration of the cell membrane, as well as a fertilization inhibitor which, used e. g. in the form of a temporary contraceptive liquid placed e. g. in the male's urinary canal, or as a vaccine given to males or females, prevents fertilization. Another object of the invention is to achieve a vaccine and medicine which prevents thrombosis in men and which also pre vents inflammation and tumour of the prostate in middle-aged men. A further object of the invention is to achieve an AIDS vaccine and medicine preventing AIDS, and a pretesting device for safe and quick pretesting of the effects of said substances outside the human system. The method of the invention for the production of a cell membrane penetration inhibitor is as follows: A reactant, which consists of a spermatozoon or the SP-10 mole cule, known in itself, found at the end of a spermatozoon, and/or of protein PH 20 or equivalent, such as a synthetically produced substance, is brought into contact with a second reac tant, which is a substance found on the surface of the ovum and/or involved in the zona reaction of the ovum or an equiva lent substance, such as a synthetically produced substance, the combination of said reactants leading to the formation of a substance inhibiting penetration of the cell membrane. Molecules and/or cells, such as white blood cells or equivalent, can be manipulated with the inhibitor or equivalent produced by the above method or equivalent so as to make them function like an inhibitor, such as the blood to be given to a person in a blood transfusion is brought into contact with the inhibitor outside the human system. The inhibitor preventing penetration of the egg's cell membrane, produced by the above or equivalent method, may be implemented as a contraceptive preventing fertilization, e. g. a contraceptive liquid for temporary use and/or a contraceptive vaccine and/or a contraceptive pill, e. g. for use by men, too. Said contraceptive or equivalent may contain an antibody of the SP 10 molecule known in itself and/or an antibody of the PH 20 protein and/or an antibody of ATP known in itself, guanosine triphosphate or equivalent. Said method or equivalent may be used to produce a substance inhibiting penetration of the cell membrane of red blood cells and/or blood platelet cells, said substance being implemented as a liquid or equivalent, e. g. as a pill, for use as a thrombosis vaccine and/or medicine. The above-mentioned thrombosis vaccine and/or medicine may contain the antibody P1G2 or equivalent of thromboxan TxA2 known in itself, and/or it may contain an antibody of ATP, guanosine triphosphate or equivalent, known in itself. A prostate vaccine and/or medicine may be produced by the method described above or by an equivalent method, in the form of a substance such as a liquid or pill inhibiting penetration of the cell membrane of prostate cells, for use e. g. as a vaccine and/or medicine injected into the prostate. The above-mentioned prostate vaccine and/or medicine may contain an antibody of ATP, guanosine triphosphate or equivalent. The above-mentioned method or equivalent may be used to produce an inhibitor preventing penetration of the cell membrane of white blood cells, implemented as a liquid or equivalent, e. g. a pill, for use as an AIDS vaccine and/or medicine. The above-mentioned AIDS vaccine and/or medicine may contain an antibody of the CD-4 molecule and/or an antibody of the CD 26 molecule and/or an antibody of the ATP molecule, known in themselves, or equivalent. The device for the pretesting of a method and/or contraceptive and/or vaccine and/or medicine or equivalent as described above may have a blood-semen mixture in a ratio of 4: 1 and a propeller wheel or an air blast nozzle or equivalent which mixes and slings the blood-semen mixture onto a contact plate, and it may have an inhibitor or equivalent as defined above.

Claims: CLAIMS 1. Method to produce an inhibitor preventing penetration of a cell mebrane, characterized in that a reactant, which consists of a spermatozoon or the SP-10 molecule, known in itself, found at the end of a spermatozoon, and/or of protein PH 20 or equivalent, such as a synthetically produced substance, is brought into contact with a second reactant, which is a substance found on the surface of the ovum and/or involved in the zona reaction of the ovum or an equivalent substance, such as a synthetically produced substance, the combination of said reactants leading to the formation of a substance inhibiting penetration of the cell membrane. 2. Method as defined in claim 1, characterized in that molecules and/or cells, such as white blood cells or equivalent, are manipulated with the inhibitor or equivalent produced by the method of claim 1 so as to make them function like an inhibitor, such as the blood to be given to a person in a blood transfusion is brought into contact with the inhibitor outside the human system. 3. Inhibitor preventing penetration of an egg's cell membrane, produced by the method of claim 1 or by an equivalent method, characterized in that it is implemented as a contraceptive preventing fertilization, such as a contraceptive liquid for temporary use and/or a contraceptive vaccine and/or a contraceptive pill, e. g. for use by men. 4. Contraceptive or equivalent as defined in claim 3, characterized in that it contains an antibody of the SP 10 molecule known in itself and/or an antibody of the PH 20 protein, or equivalent. 5. Contraceptive or equivalent as defined in claim 3 and/or 4, characterized in that it contains an antibody of ATP known in itself, guanosine triphosphate or equivalent. 6. Inhibitor preventing penetration of the cell membrane red blood cells and/or blood platelet cells, produced by the method of claim 1 or by an equivalent method, characterized in that it is implemented as a liquid or equivalent, e. g. as a pill, for use as a thrombosis vaccine and/or medicine. 7. Thrombosis vaccine and/or medicine or equivalent, characterized in that it contains the antibody P1G2 of thromboxan TxA2, or equivalent, known in itself, and/or contains an antibody of ATP, guanosine triphosphate or equivalent or equivalent, known in itself. 8. Prostate vaccine and/or medicine, characterized in that it is produced by the method defined in claim 1 or by an equivalent method in the form of a substance such as a liquid or pill inhibiting penetration of the cell membrane of prostate cells, for use e. g. as a vaccine and/or medicine injected into the prostate. 9. Prostate vaccine and/or medicine as defined in claim 8, characterized in that it contains an antibody of ATP, guanosine triphosphate or equivalent. 10. Inhibitor preventing penetration of the cell membrane of white blood cells, produced by the method defined in claim 1 or by an equivalent method, characterized in that it is implemented as a liquid or equivalent, e. g. a pill, for use as an AIDS vaccine and/or medicine. 11. AIDS vaccine and/or medicine as defined in claim 10, characterized in that it contains an antibody of the CD-4 molecule and/or an antibody of the CD 26 molecule and/or an antibody of the ATP molecule, known in themselves, or equivalent. 12. Device for the pretesting of a method and/or contraceptive and/or vaccine and/or medicine or equivalent as defined in any one of the preceding claims, characterized in that the device has a blood-semen mixture in a ratio of 4: 1 and a propeller wheel or an air blast nozzle or equivalent which mixes and slings the blood-semen mixture onto a contact plate, and that it has an inhibitor or equivalent as defined in any one of the preceding claims. AMENDED CLAIMS [received by the International Bureau on 09 November 1994 (09.11.94); original claims 1-12 replaced by amended claims 1-4 (1 page)] 1. Method to produce a thrombosis and AIDS inhibitor and medicine, characterized in that a reactant (1), which consists of a spermatozoon or the SP-10 molecule, known in itself, found at the end of a spermatozoon, and/or of protein PH 20 or equiva lent, e. g. synthetically produced PH 20, is brought into con tact with a second reactant (2), which is a substance found on the surface of the ovum and/or involved in the zona reaction of the ovum or an equivalent substance, e. g. a synthetically produced substance, and the double fertilization inhibitor, known in itself, which is produced by the combination of the reactants (1 and 2) is the AIDS and thrombosis inhibitor and medicine. 2. Inhibitor preventing penetration of the cell membrane of red blood cells and/or blood platelets, produced by the method of claim 1 or by an equivalent method, characterized in that it is implemented as a liquid or equivalent, e. g. a pill, for use as a thrombosis inhibitor and/or medicine. 3. Inhibitor preventing penetration of the cell membrane of white blood cells, produced by the method of claim 1 or by an equivalent method, characterized in that it is implemented as a liquid or equivalent, e. g. a pill, for use as an AIDS inhibitor and/or medicine. 4. Device for the pretesting of the method and/or inhibitor and/or medicine according to claim 1, characterized in that the device uses a blood-semen mixture in a ratio of 4: 1 and has a propeller wheel or an air blast nozzle or equivalent which mixes and slings the blood-semen mixture onto a contact plate, and that it uses an inhibitor or equivalent as defined in one of the preceding claims.

Inventors: REIALA MAUNO SAKARI, REIALA, MAUNO, SAKARI
Applicants: REIALA MAUNO SAKARI, REIALA, MAUNO, SAKARI
Application year: 1994
Main classification: A61K39
IPC codes: A61K35, A61K38, A61K39, C07K14, C07K16