HUMAN SPASMOLYTIC POLYPEPTIDE IN GLYCOSYLATED FORM

Abstract: Human spasmolytic polypeptide (HSP) which has the amino acid sequence (I) or a functionally equivalent homologue thereof, characterized by being in glycosylated form.

Description: HUMAN SPASMOLYTIC POLYPEPTIDE IN GLYCOSYLATED FORM FIELD OF INVENTION The present invention relates to human spasmolytic polypeptide in glycosylated form, variants of human and porcine spasmolytic polypeptides and a method of producing spasmolytic polypeptides in glycosylated form. BACKGROUND OF THE INVENTION Human spasmolytic polypeptide (HSP) belongs to a family of peptides containing one or more characteristic trefoil domains [1]. The trefoil domain is made up of a sequence of 38 or 39 amino acid residues in which 6 cystein residues are linked in the configuration 1-5, 2-4 and 3-6 thus forming a characteristic trefoil structure [1]. The trefoil family of peptides consists of rat intestinal trefoil factor, ITF [2], human breast cancer associated peptide, pS2 [3,4,5], porcine, human and murine spasmolytic polypeptide (PSP, HSP, MSP) [6,7,8] and frog spasmolysins (xPl, xP2 and xP4) [8,10,11] all containing 1, 2 or 4 trefoil domains (Fig. 1) . The physiological function of the trefoil peptides is poorly understood, and so far only PSP has been studied in any detail. In the porcine pancreas, PSP is found in the acinar cells and to be secreted in large amounts (50-100 mg/ml) into the pancreatic juice upon stimulation with pancreozymin or secretin [12,13,14]. PSP is resistant to digestion by intestinal proteases in the gastrointestinal tract [12], and specific binding of PSP to rat intestinal mucosa cells and membrane preparations from these cells has been demonstrated [15,16]. In the porcine gastrointestinal tract, specific receptor-like binding to Paneth cells in the duodenum has been found [17]. These results suggest a unique intraluminal function of the peptide. A pharmacological screening has indicated that PSP has spasmolytic and gastric acid secretion inhibitory effects [18], and studies on mammalian cells have indicated a growth factor¬ like activity of PSP [19]. The DNA sequence and derived amino acid sequence of the human counterpart of porcine SP is shown in [8], Unlike PSP, human SP (Fig. 2), has been found to be expressed in the stomach, but not in the pancreas to any greater extent [8]. An increased expression of HSP and pS2 has been reported to be associated with peptic ulcers and mucosal injury in inflammatory bowel disease [20,21] indicating a possible healing function of these peptides. Only very limited amounts of HSP can be prepared by extraction of human tissue. An object of study resulting in the present invention was therefore to prepare recombinant HSP in sufficient amounts for physiological and biochemical studies of the peptide. SUMMARY OF THE INVENTION It has surprisingly been found that when recombinant HSP is produced in certain host organisms, a proportion of it is produced in glycosylated form by posttranslational modifications. The glycosylated form of HSP has not, to applicant's best knowledge, been described previously. Accordingly, the present invention relates to human spasmolytic polypeptide (HSP) which has the amino acid sequence Glu Lys Pro Ser Pro Cys Gin Cys Ser Arg Leu Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro Gly lie Thr Ser Asp Gin Cys Phe Asp Asn Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val Pro Trp Cys Phe His Pro Leu Pro Lys Gin Glu Ser Asp Gin Cys Val Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr Pro Gly lie Ser Pro Glu Glu Cys Ala Ser Arg Lys Cys Cys Phe Ser Asn Phe lie Phe Glu Val Pro Trp Cys Phe Phe Pro Asn Ser Val Glu Asp Cys His Tyr (SEQ ID NO:l) or a functionally equivalent homologue thereof, characterized by being in glycosylated form. In the present context, the term "functionally equivalent" is intended to indicate that the homologous polypeptide has a biological activity (e.g. spasmolytic effect) corresponding to that of native HSP. The term "homologue" is intended to indicate a polypeptide encoded by DNA which hybridizes to the same probe as the DNA coding for HSP under comditions of high or low stringency (e.g. as described in Sambrook et. al., Molecular Cloning; A Laboratory Manual, 2nd Ed. , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989) . More specifically, the term is intended to refer to a DNA sequence which is at least 60% homologous to the sequence encoding HSP with the amino acid sequence shown above. The term is intended to include modifications of the DNA sequence such as nucleotide substitutions which do not give rise to another amino acid sequence of the polypeptide, but which correspond to the codon usage of the host organism into which the DNA construct is introduced or nucleotide substitutions which do give rise to a different amino acid sequence and therefore, possibly, a different protein structure which might give rise to a mutant polupeptide with different properties than the native enzyme. Other examples of possible modifications are insertion of one or more codons into the sequence, addition of one or more codons at either end of the sequence, or deletion of one or more codons at either end or within the sequence. The term "glycosylated" is intended to indicate that a carbohydrate moiety is present at one or more sites of the protein molecule. It is at present contemplated that glycosylation of HSP may give rise to differences in the biological activity of the protein, for instance with respect to stability towards proteolytic enzymes in the gastrointestinal tract, solubility at gastric and/or intestinal pH compared to non-glycosylated HSP, antigenicity, half-life, tertiary structure, and targeting to receptors on appropriate cells. In another aspect, the present invention relates to a variant of a spasmolytic polypeptide (SP) which is a fragment of human spasmolytic polypeptide (HSP) or porcine spasmolytic polypeptide (PSP) comprising at least one trefoil domain. The variant SP may be provided in both glycosylated and non- glycosylated form. It is at present contemplated that such a variant may be advantageous to use instead of full-length SP because of a higher specific biological activity, increased solubility and stability, longer half-life, easier way of production, or the like. It is assumed that other spasmolytic polypeptides than HSP will, if provided with a glycosylation site, also be expressed in predominantly glycosylated form. In a further aspect, the present invention therefore relates to a method of preparing a spasmolytic polypeptide in at least 60% glycosylated form, wherein a host cell transformed with a DNA fragment encoding a spasmolytic polypeptide and capable of providing glycosylation of said spasmolytic polypeptide is cultured under conditions permitting production of said spasmolytic polypeptide and recovering the resulting spasmolytic polypeptide from the culture. DETAILED DESCRIPTION OF THE INVENTION It has been found that, at least when recombinant HSP is produced in yeast, the proportion of it that is provided in glycosylated form is in N-glycosylated form. It has further been found that glycosylation takes place at Asnl5 of the sequence shown above. In preferred embodiments of glycosylated HSP, the glycosylated side chain contains at least one hexose unit. In particular, the glycosylated side chain may contain at least one mannose unit, preferably at least five mannose units, most preferably at least ten mannose units. In one preferred embodiment of glycosylated HSP of the invention, the glycosylated side chain contains 13-17 mannose units. In other preferred embodiments, the glycosylated HSP is in addition glycosylated with at least one unit of N-acetyl glucosamine (GlcNAc) . In the currently preferred embodiment, the glycosylated HSP is glycosylated at Asnl5 with (GlcNAc) 2 (Man) 10 _ 15 . It is further contemplated to produce homologues of HSP which are provided with one or more additional glycosylation sites. Thus, the present invention also relates to HSP homologues, wherein Lys2 is replaced by Asn, Gln7 is replaced by Asn, ArglO is replaced by Asn, Gly 20 is replaced by Thr or Ser, Gly23 is replaced by Asn, lie 24 is replaced by Asn, Phe 36 is replaced by Asn, Asp 37 is replaced by Asn, Ser39 is replaced by Asn, Gln53 is replaced by Asn, Glu61 is replaced by Asn, Asp64 is replaced by Asn, Arg66 is replaced by Thr or Ser, Gly69 is replaced by Thr or Ser, Gly72 is replaced by Asn, lie 89 is replaced by Thr or Ser, Pro98 is replaced by Asn or VallOl is replaced by Thr or Ser, or a combination of two or more of these substitutions. In a currently preferred embodiment of such an HSP homologue, Asp64 is replaced by Asn, and Arg66 is replaced by Thr or Ser. It is of course understood that HSP homologues of the invention may be glycosylated in the same manner at one or more of these sites as described above for glycosylation at Asnl5. It is assumed that the trefoil structure common among spasmolytic polypeptides is important for the function of HSP and PSP. The variant human or porcine SP comprising a fragment of the full-length polypeptide should therefore include at least three disulfide bonds to provide this structure. Consequently, the variant may comprise at least a sequence of amino acids from position 8 to 46 or from position 58 to 95, each of which sequences defines a trefoil domain of HSP and PSP. As indicated above, the SP variant of the invention may be provided in non-glycosylated form. This may, for instance, be accomplished by substituting Asnl5 by another amino acid, e.g. Asp or Glu, or by substituting Thr17 by another amino acid except Ser, e.g. Ala. It is more likely, however, that one or more additional glycosylation sites will be introduced into this domain, for instance by replacing ArglO by Asn, Gly 20 by Thr or Ser, Gly23 by Asn, lie 24 by Asn, Phe 36 by Asn, Asp 37 by Asn, or Ser39 by Asn, or a combination of two or more of these substitutions. On the other hand, it may be desirable to provide the trefoil domain from position 58 to 95 with a glycosylation site lacking in this domain in native HSP and PSP. Thus, Glu61 may be replaced by Asn, Asp64 by Asn, Arg66 by Thr or Ser, Gly69 by Thr or Ser, or Gly72 is replaced by Asn, or a combination of two or more of these substitutions. In a currently preferred embodiment of the variant, Asp64 is replaced by Asn, and Arg66 is replaced by Thr or Ser. It is of course understood that variants of the invention may be glycosylated in the same manner at one or more of these sites as described above for glycosylation at Asnlδ in full- length HSP. A DNA sequence encoding HSP may suitably be isolated from a human genomic DNA library by PCR (polymerase chain reaction) cloning using primers based on the published cDNA sequence [8]. Alternatively, the DNA sequence may be prepared synthetically by established standard methods, e.g. the phosphoa idite method described by S.L. Beaucage and M.H. Caruthers, Tetrahedron Letters 22, 1981, pp. 1859-1869, or the method described by Matthes et al. , EMBO Journal 3, 1984, pp. 801-805. According to the phosphoamidite method, oligonucleotides are synthesized, e.g. in an automatic DNA synthesizer, purified, annealed, ligated and cloned in suitable vectors. The cDNA sequence shown in [8] may be used as the basis of oligonucleotide synthesis. Alternatively, it is possible to use cDNA coding for HSP obtained by screening a human cDNA library with oligonucleotide probes in accordance with well-known procedures. Furthermore, the DNA sequence may be of mixed synthetic and genomic, mixed synthetic and cDNA or mixed genomic and cDNA origin prepared by ligating fragments of genomic, synthetic or cDNA origin (as appropriate) , the fragments corresponding to various parts of the entire DNA sequence, in accordance with standard techniques. The SP variant of the invention may be encoded by a fragment of the full-length DNA sequence, prepared by one of the methods indicated above, or by suitably truncating the full-length sequence. The DNA sequence encoding HSP or an SP variant of the invention may then be inserted in a suitable expression vector. The recombinant expression vector may be any vector which may conveniently be subjected to recombinant DNA procedures, and the choice of vector will often depend on the host cell into which it is to be introduced. Thus, the vector may be an autonomously replicating vector, i.e. a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g. a plasmid. Alternatively, the vector may be one which, when introduced into a host cell, is integrated into the host cell genome and replicated together with the chromosome(s) into which it has been integrated. In the vector, the DNA sequence encoding HSP or an SP variant of the invention should be operably connected to a suitable promoter sequence. The promoter may be any DNA sequence which shows transcriptional activity in the host cell of choice and may be derived from genes encoding proteins either homologous or heterologous to the host cell. Examples of suitable promoters for directing the transcription of the DNA encoding the inhibitor of the invention in mammalian cells are the SV 40 promoter (Subramani et al., Mol. Cell Biol. 1 , 1981, pp. 854- 864) , the MT-1 (metallothionein gene) promoter (Palmiter et al., Science 222. 1983, pp. 809-814) or the adenovirus 2 major late promoter. Suitable promoters for use in yeast host cells include promoters from yeast glycolytic genes (Hitzeman et al., J. Biol. Chem. 255, 1980, pp. 12073-12080; Alber and Kawasaki, J. Mol. APPI. Gen. 1, 1982, pp. 419-434) or alcohol dehydrogenase genes (Young et al., in Genetic Engineering of Microorganisms for Chemicals (Hollaender et al, eds.), Plenum Press, New York, 1982), or the TPI1 (US 4, 599, 311) or ADH2-4c (Russell et al., Nature 304, 1983, pp. 652-654) promoters. Suitable promoters for use in filamentous fungus host cells are, for instance, the ADH3 promoter (McKnight et al., The EMBO J___ 4 . , 1985, pp. 2093-2099) or the tpiA promoter. The "DNA sequence encoding HSP or an SP variant may also be operably connected to a suitable terminator, such as the human growth hormone terminator (Palmiter et al., op. cit. ) or (for fungal hosts) the TPI1 (Alber and Kawasaki, op. cit. ) or ADH3 (McKnight et al., op. cit.) promoters. The vector may further comprise elements such as polyadenylation signals (e.g. from SV 40 or the adenovirus 5 Elb region) , transcriptional enhancer sequences (e.g. the SV 40 enhancer) and translational enhancer sequences (e.g. the ones encoding adenovirus VA RNAs) . The recombinant expression vector may further comprise a DNA sequence enabling the vector to replicate in the host cell in question. An examples of such a sequence (when the host cell is a mammalian cell) is the SV 40 origin of replication, or (when the host cell is a yeast cell) the yeast plasmid 2μ replication genes REP 1-3 and origin of replication. The vector may also comprise a selectable marker, e.g. a gene the product of which complements a defect in the host cell, such as the gene coding for dihydrofolate reductase (DHFR) or one which confers resistance to a drug, e.g. neomycin, hygromycin or methotrexate, or the Schizosaccharo yces po be TPI gene (described by P.R. Russell, Gene 40, 1985, pp. 125-130. The procedures used to ligate the DNA sequences coding for HSP or the SP variant, the promoter and the terminator, respectively, and to insert them into suitable vectors containing the information necessary for replication, are well known to persons skilled in the art (cf. , for instance, Sambrook et al., Molecular Cloning; A Laboratory Manual, Cold Spring Harbor, New York, 1989) . The host cell into which the expression vector is introduced may be any cell which is capable of producing the inhibitor of the invention and is preferably a eukaryotic cell, such as a mammalian, yeast or fungal cell. The yeast organism used as the host cell may be any yeast organism which, on cultivation, produces large quantities of the inhibitor of the invention. Examples of suitable yeast organisms are strains of the yeast species Saccharomyces cerevisiae, Saccharomyces kluvveri, Schizosaccharomyces pombe or Saccharomyces uvarum. The transformation of yeast cells may for instance be effected by protoplast formation followed by transformation in a manner known per se. Examples of suitable mammalian cell lines are the COS (ATCC CRL 1650), BHK (ATCC CRL 1632, ATCC CCL 10) or CHO (ATCC CCL 61) cell lines. Methods of transfecting mammalian cells and expressing DNA sequences introduced in the cells are described in e.g. Kaufman and Sharp, J. Mol. Biol. 159 , 1982, pp. 601- 621; Southern and Berg, J. Mol. Appl . Genet. 1 , 1982, pp. 327- 341; Loyter et al., Proc. Natl. Acad. Sci. USA 79 , 1982, pp. 422-426; igler et al., Cell 14, 1978, p. 725; Corsaro and Pearson, Somatic Cell Genetics 1_, 1981, p. 603, Graham and van der Eb, Virology 52, 1973, p. 456; and Neumann et al. , EMBO J. I, 1982, pp. 841-845. Alternatively, fungal cells may be used as host cells. Examples of suitable fungal cells are cells of filamentous fungi, e.g. Asperαillus spp. or Neurospora spp. , in particular strains of Aspergillus orvzae or Aspergillus niger. The use of Aspergillus spp. for the expression of proteins is described in, e.g., EP 238 023. According to the present method, yeast cells are currently preferred for producing HSP and other SPs (such as those shown in Fig. 1) , as they have surprisingly been found to produce SP in a high yield and in at least 60% glycosylated form. For instance, about two thirds of the HSP produced by yeast may be recovered in glycosylated form. The medium used to cultivate the cells may be any conventional medium suitable for growing mammalian cells or fungal (including yeast) cells, depending on the choice of host cell. The spasmolytic polypeptide will be secreted by the host cells to the growth medium and may be recovered therefrom by conventional procedures including separating the cells from the medium by centrifugation or filtration, precipitating the proteinaceous components of the supernatant or filtrate by means of a salt, e.g. ammonium sulfate, purification by a variety of chromatographic procedures, e.g. ion exchange chromatography or affinity chromatography, or the like. The present invention also relates to a pharmaceutical composition comprising HSP or a variant spasmolytic polypeptide of the invention together with a pharmaceutically acceptable carrier or excipient. In the composition of the invention, the variant may be formulated by any of the established methods of formulating pharmaceutical compositions, e.g. as described in Remington's Pharmaceutical Sciences, 1985. The composition may typically be in a form suited for oral or rectal administration and may, as such, be formulated as tablets or suppositories. HSP or an SP variant of the invention is contemplated to be useful for the prophylaxis or treatment of gastrointestinal disorders. More specifically, it is contemplated for the treatment of gastric or peptic ulcers, inflammatory bowel disease, Crohn's disease or injury to the intestinal tract caused by radiation therapy, bacterial or other infections, etc. BRIEF DESCRIPTION OF THE DRAWINGS The invention is further illustrated in the following example with reference to the appended drawings in which Fig. 1 shows the trefoil family of peptides. Intestinal trefoil factor (ITF) contains one trefoil domain [2], as does the breast cancer associated pS2 peptide [3,4], The spasmolytic polypeptides from man, pig and mouse contain two trefoil domains [1,8]. Spasmolysins from Xenopus laevis contain one or four trefoil domains [10]. Recently, a member of the frog trefoil family containing two domains has been described [11]. Fig. 2 shows the proposed structure of human spasmolytic polypeptide, HSP. The primary amino acid sequence is taken from Tomasetto et al. [8], and the disulphide bonds are placed in homology to PSP [1]. Fig. 3 shows the nucleotide sequence (SEQ ID NO:2) and corresponding amino acid sequence (SEQ ID NO: 3) of the 563 bp EcoRI - Xbal fragment encoding the leader - HSP fusion protein. The Kex 2 processing site is indicated by a vertical arrow. The leader and the PCR cloned parts of the HSP gene are shown in capital letters, while the synthetic parts are shown i small letters. The underlined sequences correspond to the PCR primers with horizontal arrows indicating the direction. Restriction sites relevant for the construction are shown. Fig. 4 shows the S. cerevisiae plasmid for the expression and secretion of HSP. TPI-prom. and TPI-term. are S. cerevisiae triosephosphate isomerase transcription promoter and terminator sequences, respectively. POT is a selective marker, the Schizosaccharomyces pombe triosephosphate isomerase gene. Only restriction sites relevant for the construction of the plasmid have been indicated. Fig. 5 shows reversed-phase HPLC on a Vydac 214TP54 column of yeast fermentation broth. The two peaks corresponding to r-HSP and glycosylated r-HSP are indicated. The dashed line shows the concentration of acetonitrile in the eluting solvent. Fig. 6 shows ion exchance chromatography on a Fast Flow S column of concentrated yeast supernatant. The amount of r-HSP and glycosylated r-HSP were determined by the use of the HPLC system shown in Fig. 5. The bars indicate the fractions pooled for further purification of r-HSP and glycosylated r-HSP. The dashed line shows the concentration of NaCl in the eluting solvent. For details, see Material and Methods. Fig. 7 shows the final purification of r-HSP (A) and glycosylated r-HSP (B) on a preparative reversed-phase HPLC Vydac 214TP1022 column. The bars indicate the fractions pooled for lyophilization. The dashed lines show the concentration of acetonitrile in the eluting solvent. For details, see Material and Methods. Fig. 8 shows reversed-phase HPLC on a Vydac 214TP54 column of purified, glycosylated r-HSP (A) and r-HSP (B) . The dashed lines show the concentration of acetonitrile in the eluting solvent. Fig. 9 shows mass spectra of purified r-HSP (A and B) and glycosylated r-HSP (C and D) . Fig. A and Fig. C show the original mass spectrum of r-HSP and glycosylated r-HSP, respectively. Fig. B and Fig. D show the reconstructed mass spectrum for r-HSP and glycosylated r-HSP on the basis of Fig. A and Fig. C. EXAMPLE MATERIAL AND METHODS General methods Standard DNA techniques were used as previously described [29] . Synthetic oligonucleotides were prepared on an automatic DNA synthesizer (380B, Applied Biosystems) using commercially available reagents. DNA sequence determinations were performed by the dideoxy chain-termination technique [30]. Polymerase chain reactions (PCR) were performed on a DNA Thermal Cycler (Perkin Elmer Cetus) using a commercial kit (GeneAmp, Perkin Elmer Cetus) . PCR cloning of HSP The first trefoil domain of HSP was isolated by a PCR reaction in which 1 μg human genomic DNA (Clontech, Palo Alto, CA, USA) was used as a template. The reaction mixture contained 100 pmole each of the forward primer 1 (GGCTGAGCCCCCATAACAG) (SEQ ID NO:4) and reverse primer 2 (TGGAAACACCAGGGGAC) (SEQ ID NO:5) (Fig. 3) and was carried out in a 100 μl volume. The cycle was : 94°C for 1 min, 50°C for 1 min, and 72°C for 1 min. After 30 cycles a final cycle was performed in which the 72°C step was maintained for 10 min. The PCR product, a 115 bp fragment, was isolated by electrophoresis on a 2% agarose gel. The 115 bp PCR fragment was digested with Ddel and then ligated to a 31 bp duplex formed from the oligonucleotides (GAGAAACCCTCCCCCTGCCAGTGCTCCAGGC) (SEQ ID NO:6) and (TCAGCCTGGAGCACTGGCAGGGGGAGGGTTTCTC) . The ligation product was amplified by PCR using forward primer 3 (GCTGAGAGATTGGAGAAGAGAGAGAAACCCTCCCCCT) (SEQ ID NO:7) and reverse primer 2. The 3' part of primer 3 is identical to the N-terminal encoding part of the HSP gene and the 5' part of primer 3 is identical to the C-terminal encoding part of the hybrid leader gene (Fig. 3) . In-frame fusion of the hybrid leader gene and the first trefoil domain from HSP was obtained by overlay extension PCR [31] . The product was digested with EcoRI and Avail and isolated as a 360 bp DNA fragment. The second trefoil domain of HSP was PCR-cloned from human genomic DNA as described for the first domain by replacing primers 1 and 2 with forward primer 4 (TGCGTCATGGAGGTCTC) (SEQ ID NO:8) and reverse primer 5 (AGCACCATGGCACTTCAAAG) (SEQ ID NO:9) (Fig. 3). Reverse primer 5 introduces a Ncol site as a silent mutation. The PCR product, a 115 bp fragment, was isolated and digested with Ddel and Ncol resulting in a 91 bp fragment. To this fragment were ligated two synthetic duplexes. The first, encoding the amino acid sequence between the two trefoil domains, consisted of the oligonucleotides (GTCCCCTGGTGTTTCCACCCCCTCCCAAAGCAAGAGTCGGATCAGTGCGTCATGGAGGTC) (SEQ ID NO:10) and (TGAGACCTCCATGACGCACTGATCCGACTCTTGCT- TTGGGAGGGGGTGGAAACACCAGGG) (SEQ ID NO:11). The second, a 46 bp Ncol - Xbal fragment encoding the C-terminal part of HSP, consisted of the oligonucleotides (CATGGTGCTTCTTCCCGAACTCTGT- GGAAGACTGCCATTACTAAGT) (SEQ ID NO:12) and (CTAGACTTAGTAATGGCAGTCTTCCACAGAGTTCGGGAAGAAGCAC) (SEQ ID NO:13). After Avail digestion a 195 bp Avail - Xbal fragment was isolated. A DNA construct encoding the hybrid leader fused in-frame to the entire HSP gene was obtained by ligation of the 360 bp EcoRI - Avail fragment and the 195 bp Avail - Xbal fragment described above to the 2.7 kb EcoRI - Xbal fragment from vector pTZ19R [32]. This construct was then transformed into E. coli strain MT-172 (r " , m + ) by selection for resistance to ampicillin. DNA sequencing of the resulting plasmid, KFN-1843, showed that the correct construction had been obtained. Construction of the HSP secreting yeast strain Plasmid KFN-1843 described above was digested with EcoRI and Xbal. The resulting 558 bp fragment was isolated and ligated to the 9.3 kb Ncol - Xbal fragment and the 1.6 kb Ncol - EcoRI fragment both from the yeast expression vector pMT-636. Plasmid pMT-636 is derived from the S. cerevisiae - E. coli shuttle vector CPOT [25,33] by deletion of the 0.4 kb Hpal - Nrul fragment from the Leu-2 gene. The ligation mixture was trans¬ formed into E. coli strain MT-172, and the HSP expression plasmid, KFN-1847, was isolated (Fig. 4). Plasmid pKFN-1847 was transformed into S. cerevisiae strain MT-663 by selection for growth on glucose as the sole carbon source. One transformant, KFN-1852, was selected for fermentation. Fermentation The transformant described above was cultivated at 30°C for 3 days in yeast peptone dextrose (YPD) medium [40] supplied with additional yeast extract (60 g/1) . An OD 650 nm value of 52 was reached at the end of the fermentation. Purification of r-HSP The concentration of r-HSP in the yeast fermentation broth and fractions obtained during the purification was measured by analytical HPLC. Aliquots (usually 50-200 μl) were injected onto a Vydac 214TP54 reverse-phase C4 HPLC column (0.46 x 25 cm) equilibrated at 30"C at a flow rate of 1.5 ml/min with 0.1% (v/v) TFA in 5% (v/v) acetonitrile. The concentration of acetonitrile in the eluting solvent was raised to 65% (v/v) over 30 min. Absorbance was measured at 280 nm. The peaks eluting at 15.6 min. and 16.1 min. (Fig. 5) was found by mass spectrometry analysis to represent glycosylated r-HSP and unglycosylated r-HSP, respectively. The peptides were quantified using a calibrated PSP sample as standard as both peptides contain two Trp and two Tyr out of 106 amino acid residues. From a 10 litre fermentor, 8 litres of fermentation broth was isolated by centrifugation at 3,000 rp for 10 min. The supernatant was concentrated to 0.9 litre using an Amicon ultrafiltration unit (RA 2000) equipped with an Amicon spiral ultrafiltration cartridge type S1Y3, MW cutoff 3,000 (Product No. 540620) . The pH was adjusted to 1.7 and the conductivity in the resulting concentrated sample was measured to 4.7 mS. The sample was pumped onto a Fast Flow S-Sepharose (Pharmacia) column (5 x 11 cm) with a flow rate of 40 ml/h. Previous to the application, the column was equilibrated in 50 mM formic acid buffer, pH = 3.7. After application of the sample, the column was washed with 500 ml of 50 mM formic acid buffer, pH = 3.7. The peptides were eluted from the column by a linear gradient between 1.5 litres of 50 mM formic acid buffer, pH = 3.7 and 1.5 litres of 50 mM formic acid buffer, pH = 3.7 containing 0.6 M NaCl. Fractions of 10 ml was collected at a flow rate of 40 ml/h and the absorbance was measured at 280 nm. Fractions were assayed for the content of r-HSP and glycosylated r-HSP in the HPLC-system previously described. The elution profile is shown in Fig. 6. Fractions corresponding to r-HSP (fract. Nos. 107- 128) and glycosylated r-HSP (fract. Nos. 78-95), respectively, were pooled. Glycosylated r-HSP and r-HSP were further purified by preparative HPLC chromatography. Pooled fractions (approx. 200 ml) were pumped onto a Vydac 214TP1022 C4 column (2.2 x 25 cm) equilibrated in 0.1% (v/v) TFA. The column was washed with 100 ml of 0.1% (v/v) TFA in 10% (v/v) MeCN. The peptides were eluted at 25°C and at a flow rate of 5 ml/min with a linear gradient (650 ml) formed from MeCN/H 2 0/TFA (10.0:89.9:0.1 v/v/v) and MeCN/H 2 0/TFA (60.0:39.9:0.1 v/v/v). UV-absorption was monitored at 280 nm, and fractions corresponding to 10 ml were collected and analysed for the content of r-HSP or glycosylated r-HSP. Fig. 7 shows the preparative HPLC purification of r-HSP (Fig. 7A) and glycosylated r-HSP (Fig. 7B) . Fractions cor¬ responding to the bars were pooled, and the volume reduced to 30% by vacuum centrifugation. From the two resulting pools, r- HSP and glycosylated r-HSP were isolated by lyophilization. Characterization of r-HSP and glycosylated r-HSP Amino acid composition analysis were carried out by hydrolysis of 50 μg peptide with 6M HCl for 24 h at 110"C as previously described [6]; no correction for loss during hydrolysis was carried out. Amino acid sequence analysis was determined by automated Edman degradation using an Applied Biosystems Model 470A gas-phase sequencer [22]. Carbohydrate composition analysis was carried out by hydrolysis of 50 μg peptide with 2M HCl for lh, 2h and 4h at 100'C and monosaccharides were separated on a CarboPac PAI (Dionex, Sunnyvale, CA) column (4 x 250 mm) eluted with 14 mM NaOH. The monosaccharides were detected by pulsed amperometric detection (Dionex PAD- detector) . The amount of monosaccharides was corrected to zero time of hydrolysis and calculated as nmol of monosaccharide per nmol of peptide. Mass spectro etry analysis was performed using an API III LC/MS/MS system (Sciex, Thornhill, Ontario, Canada). The triple quadrupole instrument has a mass-to-charge (m/z) range of 2400 and is fitted with a pneumatically assisted electrospray (also referred to as ion-spray) interface [23,24]. Sample introduction was done by a syringe infusion pump (Sage Instruments, Cambridge, MA) through a fused capillary (75 μm i.d.) with a liquid flow-rate set at 0.5-1 μl/min. The instrument m/z scale was calibrated with the singly-charged am- monium adduct ions of poly(propylene glycols) (PPG's) under unit resolution. The accuracy of mass measurements was generally better than 0.02%. RESULTS Expression and purification DNA fragments encoding the two trefoil domains of HSP were isolated by PCR from human genomic DNA using primers based on the published cDNA sequence [8], The full length HSP gene was obtained from the PCR cloned fragments by addition of synthetic DNA fragments. The HSP gene was fused in-frame to a hybrid yeast leader sequence by overlay extension PCR [31] (Fig. 3) . The hybrid leader is based on the mouse salivary amylase signal peptide [34] and the S. kluvveri α mating factor leader sequence [35] and is further modified near the Kex 2 cleavage site for efficient processing [36, 41]. The yeast expression plasmid pKFN-1847 contains the leader-HSP gene inserted between the S. cerevisiae triose phosphate isomerase promoter and terminator [37] . The expression vector (Fig. 4) also contains the Schizosaccharomyces pombe TPI gene (POT) [38]. The plasmid was transformed into the yeast strain MT-663, carrying a deletion in the TPI gene, by selecting for growth on glucose. The expression level of r-HSP in the present yeast system is approx. 120 mg/1. As can be seen from Fig. 5, the yeast su¬ pernatant contains two forms of r-HSP; one eluting at R t = 15.6 min. and one eluting at R t = 16.1 min. These two forms were purified separately, and by using the analytical HPLC-system (Fig. 5) , these two forms can be quantified individually during the different steps of the purification. After the initial concentration of the yeast supernatant by ultrafiltration, the first purification step was cationic exchange chromatography on a Fast Flow S column. Fig. 6 shows the elution profile from the column including the amount of r- HSP and glycosylated r-HSP determined in the fractions. A complete separation of the two forms of r-HSP was obtained in this step. The fractions from the Fast Flows S column were pooled as 5 indicated in Fig. 6, and the two peptides were further purified by preparative HPLC (Fig. 7) . The r-HSP and glycosylated r-HSP were recovered from the fractions indicated in Fig. 7A and Fig. 7B by vacuum centrifugation and lyophilization. The purification is summarized in Table 1. The overall yield of r- 10 HSP and glycosylated r-HSP from 8 litres of fermentation broth was 160 mg and 219 mg corresponding to 50% and 34%, respectively. Characterization of r-HSP and glycosylated r-HSP Fig. 8 shows the purity of r-HSP and glycosylated r-HSP as 15 analysed by analytical HPLC. From these results none of the peptides looks completely pure. However, upon rechromatography of material eluting in the minor as well as the major peak, similar chromatograms were obtained for both peptides (results not shown) . This seems to indicate that the double peak 20 observed for both r-HSP and glycosylated r-HSP reflects an atypical behaviour of these peptides on reverse phase columns rather than impurities in the preparations. Table 2 shows the amino acid sequencing results obtained on r- HSP and glycosylated r-HSP. The average repetitive yield was 25 94.4% (r-HSP) and 94.6% (glycosylated r-HSP) , respectively. In both cases the first 40 residues of the two peptides were confirmed by the sequence analysis. In the glycosylated HSP, no PTH-a.a. was found in Edman degradation cycle No. 15. The HSP sequence from residue 15-17 (Asn-Arg-Thr) corresponds to a 30 consensus sequence for N-glycosylation of Asn-15. The carbohydrate composition analysis of glysocylated r-HSP showed the presence of 12.8 nmol mannose (Man) and 1.6 nmol of N-acetyl glucoseamine (GlcNAc) per nmol of r-HSP. By peptide mapping of r-HSP and glycosylated r-HSP in combination with mass spectrometry and sequencing analysis (results not shown) , no other residue besides Asn-15 of the glycosylated r-HSP was found to be modified, i.e. no O-glycosylation was found. In Fig. 9, the electro-spray mass spectrometry (ESMS) analysis is shown for r-HSP and glycosylated r-HSP. Fig. 9A and 9C are original mass spectra displaying characteristics series of multiply charged protonated ions always observed in ESMS spectra of proteins. Fig. 9B and 9D are the corresponding computer reconstructed mass spectra from which the molecular weight of individual components may be read directly. As can be seen from Fig. 9B, the MW found for r-HSP is 11961.5 ±2 which is in very good agreement with a calculated mass of 11961.3. Fig. 9D shows the reconstructed ion spray mass spectrum of the glycosylated r-HSP. From the sequence analysis and the carbohydrate composition analysis, it is known that only Asn-15 is glycosylated and that only two monosaccharide residues, mannose and N-acetyl glucoseamine, occur in the glycosylated form of r-HSP. From these results in combination with the mass spectrometry data, it is possible to deduce the different glycosylated forms of r-HSP (Table 3) . Molecular weights corresponding to two series of carbohydrate side chains can be deduced from the combination of carbohydrate composition data and ISMS-data, namely (GlcNAc) -(Hex) 10 . 15 and (Hex) 13 . 17 (Table 3) . As mannose is the only hexose in the glyco¬ sylated r-HSP, and as Asn-15 is the only glycosylated residue, it seems reasonable to conclude that the structure of the glycosylation site is Asn-(GlcNAc) 2 -(Man) 10 . 15 . The observed Asn- (Hex) 13 . 17 forms are thus most likely to arise from fragmentation in the mass spectrometer, by which the two GlcNAc residues lose an acetyl group and are converted into two hexoses. The structure of Asn-(GlcNAc) 2 -(Man) 10 . 15 has previously been reported as high mannose type of N-glycosylation for other peptides and proteins expressed in yeast [26]. Table 1 5 Purification of r-hSP and glycosylated r-hSP from yeast supernatant Amount [mg] Yield [%] STEP Volume [ml] r-hSP glycosylated r-hSP glycosylated r-hSP r-hSP Yeast supernatant 8000 320 640 100 100 Ultrafiltration 900 207 405 65 63 Ion exchange Pool 1 160 275 43 chromatography Pool 2 220 182 57 Prep HPLC Pool 1 54 219 34 Pool 2 80 160 50 Table 2 Amino acid sequence analysis of r-hSP and glycosylated r-hSP Yield (pmol) Cycle No. PTH-a.a. r-hSP glycosylated r- hSP 1 Glu 4304 8853 2 Lys 6925 8292 3 Pro 6027 12837 4 Ser 2890 5602 5 Pro 4336 8802 6 (Cys) ND ND 7 Gin 3388 5689 8 (Cys) ND ND 9 Ser 1279 2417 10 Arg 1876 2523 11 Leu 2277 4290 12 Ser 877 1790 13 Pro 1545 2963 14 His 517 574 15 Asn 1202 0* 16 Arg 959 1471 17 Thr 978 2172 18 Asn 1066 1509 19 (Cys) ND ND 20 Gly 836 1857 21 Phe 993 1958 22 Pro 843 1839 23 Gly 785 2049 24 He 640 1400 25 Thr 589 1454 26 Ser 274 621 27 Asp 581 1391 28 Gin 445 952 29 (Cys) ND ND 30 Phe 623 1562 31 Asp 483 1210 32 Asn 369 823 33 Gly 359 885 34 (Cys) ND ND 35 (Cys) ND ND 36 Phe 422 1094 37 Asp 268 783 38 Ser 127 324 39 Ser 145 394 40 Val 298 827 ND: Not deter mined *: No trace of PTH-Asn or PTH-Asp was seen in cycle No. 15 of glycosylated r-hSP. Table 3 Mass analysis of glycosylated r-hSP Structure Calculated MW found by MW ESMS (Fig.9D) hSP + 2 GlcNAc + 10 Man 13989.1 13989.5 hSP + 2 GlcNAc + 11 Man 14151.2 14151.0 hSP + 2 GlcNAc + 12 Man 14313.4 14313.5 hSP + 2 GlcNAc + 13 Man 14475.5 14475.0 hSP + 2 GlcNAc + 14 Man 14639.7 14639.5 hSP + 2 GlcNAc + 15 Man 14799.8 14801.5 hSP + 13 Man 14069.1 14072.0 hSP + 14 Man 14231.3 14232.5 hSP + 15 Man 14393.4 14393.0 hSP + 16 Man 14555.5 14557.5 hSP + 17 Man 14717.7 14720.0 REFERENCES [1] Thim, L. (1989) FEBS Lett. 250, 85-90. [2] Suemori, S., Lynch-Devaney, K. and Podolsky, D.K. (1991) Proc. Natl. Acad. Sci. USA 88, 11017-11021. [3] Jakowlew, S.B., Breathnach, R. , Jeltsch, J.M. , Masiakowski, P. and Chambon, P. (1984) Nucleic Acids Res. 12, 1861-2878. [4] Prud'homme, J.-F., Fridlansky, F., Le Cunff, M. , Atger, M. , Mercier-Bodart, C. , Pichon, M.-F. and Milgrom, E. (1985) DNA 4, 11-21. [5] Rio, M.C., Bellocq, J.P., Daniel, J.Y., Tomasetto, C. , Lathe, R. , Chenard, M.P., Batzenschlager, A. and Chambon, P. (1988) Science 241, 705-708. [6] Thim, L. , Thomsen, J., Christensen, M. and Jørgensen, K.H. (1985) Biochim. Biophys. Acta 827, 410-418. [7] Rose, K. , Savoy, L.-A., Thim, L. , Christensen, M. and Jørgensen, K.H. (1989) Biochim. Biophys. Acta 998, 297-300. [8] Tomasetto, C. , Rio, M.-C, Gautier, C. , Wolf, C. , Hareuveni, M. , Chambon, P. and Lathe, R. (1990) EMBO J. 9, 407-414. [9] Hoffmann, W. (1988) J. Biol. Chem. 263, 7686-7690. [10] Hauser, F. and Hoffmann, W. (1991) J. Biol. Chem. 266, 21306-21309. [11] Hauser, F. , Roeben, C. and Hoffmann, W. (1992) J. Biol. Chem. 267, 14451-14455. [12] Jørgensen, K.H., Thim, L. and Jacobsen, H.E. (1982) Regul. Peptides 3, 207-219. [13] Thim, L. , Jørgensen, K.H. and Jørgensen, K.D. (1982) Regul. Peptides 3, 221-230. [14] Rasmussen, T.N., Raabjerg, L. , Poulsen, S.S., Thim, L. and Hoist, J.J. (1992) Histochemistry 98, 113-119. [15] Frandsen, E.K., Jørgensen, K.H. and Thim, L. (1986) Regul. Peptides 16, 291-297. [16] Frandsen, E.K. (1988) Regul. Peptides 20, 45-52. [17] Rasmussen, T.N., Raabjerg, L. , Poulsen, S.S., Thim, L. and Hoist, J.J. (1992) Am. J. Physiol. (in press) [18] Jørgensen, KD. , Diamant, B. , Jørgensen, K.H. and Thim, L. (1982) Regul. Peptides 3, 231-243. [19] Hoosein, N.M. , Thim, L. , Jørgensen, K.H. and Brattain, M.G. (1989) FEBS Lett. 247, 303-306. [20] Wright, N.A. , Poulsom, R. , Stamp, G.W. , Hall, P.A. , Jeffery, R.E., Longcroft, J.M., Rio, M.-C, Tomasetto, C and Chambon, P. (1990) J. Pathol. 162, 279-284. [21] Rio, M.-C, Chenard, M.-P., Wolf, C, Marcellin, L. , Tomasetto, C, Lathe, R. , Bellocq, J.P. and Chambon, P. (1991) Gastroenterology 100, 375-379. [22] Thim, L. , Hansen, M.T. and Soerensen, A.R. (1987) FEBS Lett. 212, 307-312. [23] Bruins, A.P., Covey, T.R. and Henion, J.D. (1987) Anal. Chem. 59, 2642-2646. [24] Covey, T.R., Bonner, R.F., Shushan, B.I. and Henion, J.D. (1988) Rapid Co mun. Mass Spectrom. 2, 249-256. [25] Thim, L. , Hansen, M.T., Norris, K. , Hoegh, I., Boel, E., Forstrom, J., Ammerer, G. and Fiil, N.P. (1986) Proc. Natl. Acad. Sci. USA 83, 6766-6770. [26] Poulter, L. and Burlingame, A.L. (1990) in: Methods in Enzy ology (McCloskey, J.A., ed.) 193, 661-689. Academic Press, Inc., San Diego, CA. [27] Gajhede, M. , Thim, L. , Jørgensen, K.H. and Melberg, S.G. (1992) Proteins: Structure, Function, and Genetics 13, 364-368. [28] Carr, M.D. (1992) Biochemistry 31, 1998-2004. [29] Sambrook, J., Fritch, E. F. , and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. [30] Sanger, F. , Micklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467. [31] Horton, R. M. , Hunt, H. D. , Ho, S. N. , Pullen, J. K. , and Pease, L. R. (1989) Gene 77, 61-68. [32] Mead, D. A., Szczesna-Skorupa, E. and Kemper, B. (1986) Prot. Engin. 1, 67-74. [33] Kawasaki, G. (1984) 12th International Conference on Yeast Genetics and Molecular Biology, Sept. 17-24, 1984, Edinburgh, Scotland, Abstr. P15. [34] Hagenbiichle, O., Tosi, M. , Schibler, U. , Bovey, R. , Wellauer, P. K. , and Young, R. A. (1981) Nature 289, 643-646. [35] Egel-Mitani, M. and Hansen, M. T. (1987) Nucl. Acids Res. 15, 6303-6304. [36] Christiansen, L. and Norris, K. , personal communication. [37] Alber, T. and Kawasaki, G. (1982) J. Mol. Appl. Genet. 1, 419-434. [38] Russell, P. R. (1985) Gene 40, 125-130. [39] Jeltsch, J. M. , Roberts, M. , Schatz, C, Gamier, J. M. , Brown, A. M. C, and Chambon, P. (1987) Nucl. Acids. Res. 15, 1401-1414. [40] Sherman, F. , Fink, G.R. and Hicks, J.B. (1981) Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, New York. [41] Norris, K. , Norris, F. and Bjoern, S.E. (1990) International Patent Application WO 90/10075. SEQUENCE LISTING (1) GENERAL IN-FO-RMATION: (i) APPLICANT: (A) NAME: Novo Nordisk A/S (B) STREET: Novo Alle (C) CITY: Bagsvaerd (E) COUNTRY: Denmark (F) POSTAL CODE (ZIP) : 2880 (G) TELEPHONE: +45 4444 8888 (H) TELEFAX: +45 4449 3256 (I) TELEX: 37304 (ii) TrTLE OF INVENTION: Human Spasmolytic Polypeptide in Glycosylated Form (iii) NUMBER OF SEQUENCES: 14 (iv) COMEUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentln Release #1.0, Version #1.25 (EPO) (2) INFORMATION FOR SEQ ID NO: 1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 106 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOIOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: Glu Lys Pro Ser Pro Cys Gin Cys Ser Arg Lsu Ser Pro His Asn Arg 1 5 10 15 Thr Asn Cys Gly Phe Pro Gly lie Thr Ser Asp Gin Cys Phe Asp Asn 20 25 30 Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val Pro Trp Cys Phe His 35 40 45 Pro Lsu Rro Lys Gin Glu Ser Asp Gin Cys Val Met Glu Val Ser Asp 50 55 60 Arg Arg Asn Cys Gly Tyr Pro Gly lie Ser Pro Glu Glu Cys Ala Ser 65 70 75 80 Arg Lys Cys Cys ihe Ser Asn Phe lie Phe Glu Val Pro Trp Cys ihe 85 90 95 Phe Pro Asn Ser Val Glu Asp Cys His Tyr 100 105 (2) INPORMATTON FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 563 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: Homo sapiens (ix) FEATURE: (A) NAME/KEY: CDS (B) -LOCATION: 77. .553 (ix) FEATURE: (A) NAME/KEY: sig_peptide (B) LOCATION: 77..235 (ix) FEATURE: (A) NAME/KEY: mat_peptide (B) 1--OCATTON: 236..553 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: GAATTCCATT CAAGAATAGT TCAAACAAGA AGATTACAAA CTATCAATTT CATACACAAT 60 ATAAACGACC AAAAGA ATG AAG GCT GTT TTC TTG GTT TTG TCC TTG ATC 109 Met Lys Ala Val Phe Leu Val Leu Ser Leu lie -53 -50 -45 GGA TTC TGC TGG GCC CAA CCA GTC ACT GGC GAT GAA TCA TCT GTT GAG 157 Gly Hie Cys Trp Ala Gin Rro Val Thr Gly Asp Glu Ser Ser Val Glu -40 -35 -30 ATT COG GAA GAG TCT CIG ATC ATC GCT GAA AAC ACC ACT TTG GCT AAC 205 He Pro Glu Glu Ser Lsu He He Ala Glu Asn Thr Thr Lsu Ala Asn -25 -20 -15 GTC GCC ATG GCT GAG AGA TTG GAG AAG AGA GAG AAA CCC TCC CCC TGC 253 Val Ala Met Ala Glu Arg Leu Glu Lys Arg Glu Lys Pro Ser Pro Cys -10 -5 1 5 CAG TGC TCC AGG CTG AGC CCC CAT AAC AGG AGG AAC TGC GGC TTC CCT 301 Gin Cys Ser Arg Leu Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro 10 15 20 GGA ATC ACC ACT GAC CAG TGT TTT GAC AAT GGA TGC TGT TTC GAC TCC 349 Gly He Thr Ser Asp Gin Cys Phe Asp Asn Gly Cys Cys He Asp Ser 25 30 35 ACT GTC ACT GGG GTC CCC TGG TGT TTC CAC CCC CTC CCA AAG CAA GAG 397 Ser Val Thr Gly Val Pro Trp Cys Phe His Pro Leu Pro Lys Gin Glu 40 45 50 TGG GAT CAG TGC GTC ATG GAG GTC TCA GAC AGA AGA AAC TGT GGC TAC 445 Ser Asp Gin Cys Val Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr 55 60 65 70 CCG GGC ATC AGC CCC GAG GAA TGC GCC TCT CGG AAG TGC TGC TTC TCC 493 Pro Gly He Ser Pro Glu Glu Cys Ala Ser Arg Lys Cys Cys Ehe Ser 75 80 85 AAC TTC ATC TTT GAA GTG CCA TGG TGC TTC TTC CCG AAC TCT GTG GAA 541 Asn ihe He Ehe Glu Val Pro Trp Cys Phe Phe Pro Asn Ser Val Glu 90 95 100 GAC TGC CAT TAC TAAGTCTAGA 563 Asp Cys His T"yr 105 (2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 159 amino acids (B) TYPE: amino acid (D) TOPOIDGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: Met Lys Ala Val ihe leu Val Leu Ser Leu He Gly ihe Cys Trp Ala -53 -50 -45 -40 Gin Pro Val Thr Gly Asp Glu Ser Ser Val Glu He Pro Glu Glu Ser -35 -30 -25 Leu He He Ala Glu Asn Thr Thr Leu Ala Asn Val Ala Met Ala Glu -20 -15 -10 Arg Leu Glu Lys Arg Glu Lys Pro Ser Pro Cys Gin Cys Ser Arg Leu -5 1 5 10 Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro Gly He Thr Ser Asp 15 20 25 Gin Cys Phe Asp Asn Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val 30 35 40 Pro Trp Cys Phe His Rro Lsu Pro Lys Gin Glu Ser Asp Gin Cys Val 45 50 55 Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr Pro Gly He Ser Pro 60 65 70 75 Glu Glu Cys Ala Ser Arg Lys Cys Cys Phe Ser Asn Phe He Phe Glu 80 85 90 Val Pro Trp Cys ihe Fhe Pro Asn Ser Val Glu Asp Cys His Tyr 95 100 105 (2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) IENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cENA (iii) HYPOTHETICAL: NO ( i) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: GGCTGAGCCC CCATAACAG 19 (2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: TGGAAACACC AGGGGAC 17 (2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACrERISTICS: (A) LENGTH: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: GAGAAACCCT CCCCCTGCCA GTGCTCCAGG C 31 (2) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: TCAGCCTGGA GCACTGGCAG GGGGAGGGTT TCTC 34 (2) INPORMATTON FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 37 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: GCTGAGAGAT TGGAGAAGAG AGAGAAACCC TCCCCCT 37 (2) INϊORMAπON FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) -HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: TGCGTCATGG AGGTCTC 17 (2) INFORMATTON FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: AGCACCATGG CAC TCAAAG 20 (2) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 60 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: GTCCCCTGGT GTTTCCACCC CCTCCCAAAG CAAGACTCGG ATCAGTGCCT CATGGAGGTC 60 (2) INFORMATION FOR SEQ ID NO: 12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 60 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: CDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRI-PTTON: SEQ ID NO: 12: TGAGACCTCC ATGACGCACT GATCCGACTC TTGCTTTGGG AGGGGGTGGA AACACCAGGG 60 (2) INFORMATION FOR SEQ ID NO: 13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 46 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13: CATGGTGCTT CTTCCOGAAC TCTGTGGAAG ACTGCCATTA CTAACT 46 (2) INFORMATION FOR SEQ ID NO: 14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 46 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOIDGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (vi) ORIGINAL SOURCE: (A) ORGANISM: synthetic (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14: CTAGACTTAG TAATGGCACT CTTCCACAGA GTTCGGGAAG AAGCAC 46

Claims: CLAIMS 1. Human spasmolytic polypeptide (HSP) which has the amino acid sequence Glu Lys Pro Ser Pro Cys Gin Cys Ser Arg Leu Ser Pro His Asn Arg Thr Asn Cys Gly Phe Pro Gly lie Thr Ser Asp Gin Cys Phe Asp Asn Gly Cys Cys Phe Asp Ser Ser Val Thr Gly Val Pro Trp Cys Phe His Pro Leu Pro Lys Gin Glu Ser Asp Gin Cys Val Met Glu Val Ser Asp Arg Arg Asn Cys Gly Tyr Pro Gly lie Ser Pro Glu Glu Cys Ala Ser Arg Lys Cys Cys Phe Ser Asn Phe lie Phe Glu Val Pro Trp Cys Phe Phe Pro Asn Ser Val Glu Asp Cys His Tyr (SEQ ID NO:l) or a functionally equivalent homologue thereof, characterized by being in glycosylated form. 2. HSP according to claim 1, characterized by being in N- glycosylated form. 3. HSP according to claim 1 or 2, which is glycosylated at Asnl5. 4. HSP according to claim 3, in which the glycosylated side chain contains at least one hexose unit. 5. HSP according to claim 4, in which the glycosylated side chain contains at least one mannose unit, preferably at least five mannose units, most preferably at least ten mannose units. 6. HSP according to claim 5, in which the glycosylated side chain contains 13-17 mannose units. 7. HSP according to claim 4, which in addition is glycosylated with at least one unit of N-acetyl glucosamine (GlcNAc) . 8. HSP according to claim 7, which is glycosylated with (GlcNAc) 2 (Man) 10.15 . 9. HSP according to claim 1, wherein Lys2 is replaced by Asn, Gln7 is replaced by Asn, ArglO is replaced by Asn, Gly 20 is 5 replaced by Thr or Ser, Gly23 is replaced by Asn, lie 24 is replaced by Asn, Phe 36 is replaced by Asn, Asp 37 is replaced by Asn, Ser39 is replaced by Asn, Gln53 is replaced by Asn, Glu61 ia replaced by Asn, Asp64 is replaced by Asn, Arg66 is replaced by Thr or Ser, Gly69 is replaced by Thr or Ser, Gly72 10 is replaced by Asn, lie 89 is replaced by Thr or Ser, Pro98 is replaced by Asn or VallOl is replaced by Thr or Ser, or a combination of two or more of these substitutions. 10. HSP according to claim 9, wherein Asp64 is replaced by Asn, and wherein Arg66 is replaced by Thr or Ser. 15 11. HSP according to claim 9, in which the glycosylated Asn contains at least one hexose unit. 12. HSP according to claim 11, which is glycosylated with at least one mannose unit, preferably at least five mannose units, most preferably at least ten mannose units. 20 13. HSP according to claim 12, which is glycosylated with 13-17 mannose units. 14. HSP according to claim 11, which in addition is glycosylated with at least one unit of N-acetyl glucosamine (GlcNAc) . 25 15. HSP according to claim 14, which is glycosylated with (GlcNAc) 2 (Man) 10 . 15 . 16. A variant of a spasmolytic polypeptide which is a fragment of human spasmolytic polypeptide (HSP) or porcine spasmolytic polypeptide (PSP) comprising at least one trefoil domain. 17. A variant according to claim 16, which comprises at least a sequence of amino acids from position 8 to 46 or from position 58 to 95. 18. A variant according to claim 16 or 17, wherein Asnl5 is substituted by another amino acid, e.g. Asp or Glu. 19. A variant according to claim 16 or 17, wherein Thrl7 is substituted by another amino acid except Ser, e.g. Ala. 20. A variant according to claim 16 or 17, wherein ArglO is replaced by Asn, Gly 20 is replaced by Thr or Ser, Gly23 is replaced by Asn, lie 24 is replaced by Asn, Phe 36 is replaced by Asn, Asp 37 is replaced by Asn, Ser39 is replaced by Asn, Glu61 ia replaced by Asn, Asp64 is replaced by Asn, Arg66 is replaced by Thr or Ser, Gly69 is replaced by Thr or Ser, or Gly72 is replaced by Asn, or a combination of two or more of these substitutions. 21. A method of preparing a spasmolytic polypeptide in at least 60% glycosylated form, wherein a host cell transformed with a DNA fragment encoding a spasmolytic polypeptide and capable of providing glycosylation of said spasmolytic polypeptide is cultured under conditions permitting production of said spasmolytic polypeptide and recovering the resulting spasmolytic polypeptide from the culture. 22. A method according to claim 21, wherein the host cell is a fungal cell such as a yeast cell, e.g. a strain of Saccharomyces cerevisiae. or a filamentous fungus cell, e.g. a strain of Aspergillus. such as Aspergillus orvzae. 23. A pharmaceutical composition comprising HSP according to any of claims 1-15 together with a pharmaceutically acceptable carrier or excipient. 24. A pharmaceutical composition comprising a variant spasmolytic polypeptide according to any of claims 16-20 together with a pharmaceutically acceptable carrier or excipient. 525. Use of HSP according to any of claims 1-15 for the manufacture of a medicament for the prophylaxis or treatment of gastrointestinal disorders. 26. Use of a variant spasmolytic polypeptide according to any of claims 16-20 for the manufacture of a medicament for the 0 prophylaxis or treatment of gastrointestinal disorders.

Inventors: BJORN SOREN ERIK, CHRISTENSEN MOGENS, NIELSEN PER FRANKLIN, NORRIS FANNY, NORRIS KJELD, THIM LARS, BJORN, SOREN, ERIK, CHRISTENSEN, MOGENS, NIELSEN, PER, FRANKLIN, NORRIS, FANNY, NORRIS, KJELD, THIM, LARS
Applicants: BJORN SOREN ERIK, CHRISTENSEN MOGENS, NIELSEN PER FRANKLIN, NORRIS FANNY, NORRIS KJELD, NOVONORDISK AS, THIM LARS, BJORN, SOREN, ERIK, CHRISTENSEN, MOGENS, NIELSEN, PER, FRANKLIN, NORRIS, FANNY, NORRIS, KJELD, NOVO NORDISK A/S, THIM, LARS
Application year: 1994
Main classification: C07K15
IPC codes: A61K38, C07K14, C12N15