AMIDINE DERIVATIVES WITH NITRIC OXIDE SYNTHETASE ACTIVITIES

Abstract: Compounds of formula (I) wherein D represents phenyl, pyridinyl or a 5-membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which three groups are optionally substituted by one or more groups selected from alkyl C1 to 6, alkoxy C1 to 6, halogen and perfluoroalkyl C1 to 6; or perfluoroalkyl C1 to 6; R<1> represents hydrogen, alkyl C1 to 6 or halogen; R<2> represents a group -X(CH2)nZCONR<3>R<4>, -X(CH2)nNHCO(CH2)sNR<3>R<4>, -X(CH2)pNR<3>R<4>, -X(CH2)nNHCOR<5> or -(CH2)qNHC(NH)R<6>; and X, Z, R<3>, R<4>, R<5>, R<6>, n, s, p and q are defined in the specification are described, together with processes for their preparation and compositions containing them. Compounds of formula (I) have nitric oxide synthetase inhibitory activity.

Description: Amidine derivatives wi th ni tric oxide synthetase activiti es This invention relates to amidine derivatives, processes for their preparation, compositions containing them and their use in therapy. Certain nitrogen containing compounds have been described as neuroprotective agents. International Patent Application WO 91/12797 (State of Oregon) teaches tri- and tetrasubstituted guanidines as neuroprotective agents. US Patent 5266594 (Dawson et al) (published after the earliest priority date of this application) describes the use of arginine derivatives in the treatment of stroke and other neurodegenerative diseases. Also European Patent Application 547558 (Washington University) describes the use of aminoguanidine in the treatment of immunological and other disorders. The use of inhibitors of nitric oxide synthetase in the treament of disease has also been described, for example, in International Patent Applications WO 94/12163 (Abbott) and WO 94/12165 (Wellcome) (both published after the earliest priority date of this application) and European Patent Application 446699 (Merrell Dow). Amidine derivatives have been described for use as herbicides in German Patent Application DE-OS-2321330 (Bayer). N-phenyl amidine derivatives have also been described for use in the treatment of diabetes in US Patent 3669974 (USV Pharmaceutical Corp.) and UK Patent Application 2226562 (Boots). N'N"- disubstituted amidines are described for use in the treatment of hypertension, depression and halliconogenic states in International Patent Application WO 92/04054 (University of Oregon). The use of certain symmetric bisamidines as analgesics, in the treatment of inflammation and in the treatment of hypertension is described in UK Patent No. 1180629 (Delalande). A number of. patent documents describe processes for the preparation of amidines or describe the use of amidines as intermediates without disclosing any pharmaceutical use for these compounds. Simple amidine derivatives are described in UK Patent No. 1088095 (Merck) as intermediates in the preparation of useful benzimidazole derivatives. Processes for preparation of other simple N-aryl and N- heteroaryl amidines are described in US Patent 3299081 (Merck) and fluorine containing amidine derivatives are described as chemical intermediates in Japanese Patent Application No. 2229147 (Nissan) and in Japanese Patent Application No. 58057357 (Daikin). We have now found a new group of amidine derivatives that possesses useful pharmaceutical activity. According to a first aspect of the invention, we provide a compound of formula I wherein D represents phenyl, pyridinyl or a 5 membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which three groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen and perfluoroalkyl Cl to 6; or perfluoroalkyl Cl to 6; R 1 represents hydrogen, alkyl Cl to 6 or halogen; R 2 represents -X(CH 2 ) n ZCONR 3 R\ -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R\ -X(CH 2 ) p NR 3 R 4 , -X(CH 2 ) n NHCOR 5 or -(CH 2 ) q NHC(NH)R 6 ; R 3 and R 4 independently represent hydrogen, alkyl Cl to 6, -(ClHy-A . -(CH 2 ) m OA or -CH(CH 3 )(CH 2 ) t A; or -NR 3 R 4 together represent 1-indanyl, piperonylamino-, piperidinyl, morpholinyl, pyrrolidinyl, 1,2,3,4-tetrahydroisoquinolinyl; or piperazinyl optionally 4-substituted by alkyl Cl to 6; R 5 represents alkyl Cl to 6, perfluoroalkyl Cl to 6, -(CH 2 ),A or A represents phenyl, pyridinyl, pyrimidinyl, or a 5 membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which four groups are optionally substituted by one or more groups selected from alkyl Cl to 6, halogen, nitro, cyano and trifluoromethyl; R 6 represents phenyl, pyridinyl or a 5 membered heterocyclic aromatic ring contaimng 1 to 4 heteroatoms selected from O, S and N, which three groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen and perfluoroalkyl Cl to 6; or perfluoroalkyl Cl to 6; n and r independently represent an integer in the range 0 to 6 inclusive; p and w independently represent an integer in the range 1 to 5 inclusive; m represents an integer in the range 2 to 5 inclusive; q and t independently represent an integer in the range 0 to 5 inclusive; s represents an integer in the range 1 to 3 inclusive; X represents O or a bond; Z represents O, NR 7 or a bond; R 7 represents hydrogen or alkyl Cl to 6; provided that: (a) when D contains a heteroatom, it is not connected to the remainder of the compound of formula I through the heteroatom; (b) when R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and neither X nor Z represent a bond, then n represents an integer in the range 2 to 6 inclusive; (c) when R 2 represents -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 or -X(CH 2 ) n NHCOR 5 , and X represents O, then n represents an integer in the range 2 to 6 inclusive; (d) when R 2 represents -X(CH 2 ) p NR 3 R 4 and X represents O, then p represents an integer in the range 2 to 5 inclusive; (e) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D represents phenyl and R 6 represents phenyl, then q does not represent 0; (f) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D and R 6 represent 2-chlorophenyl, then q does not represent 0; (g) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D and R 6 represent 3-pyridinyl, then q does not represent 0; and (h) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D and R 6 represent 4-pyridinyl, then q does not represent 0; or a pharmaceutically acceptable salt thereof. We prefer that D represents phenyl, pyridinyl or a 5 membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which three groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen or perfluoroalkyl Cl to 6. We particularly prefer that D represents phenyl, thiophene, furan, pyrrole or thiazole, which five groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen or perfluoroalkyl Cl to 6. We more particularly prefer that D represents thiophene, pyrrole, furan or thiazole which four groups are optionally substituted by alkyl Cl to 6 or halogen. We especially prefer than D represents thiophene, furan or pyrrole, most especially thiophene. We prefer most of all that D represents 2-thiophene. We prefer that R 1 represents hydrogen. When R 2 represents -X(CH 2 ) n ZCONR 3 R 4 , -X(CH 2 ) B NHCO(CH 2 ) s NR 3 R 4 or -X(CH 2 ) p NR 3 R 4 , we prefer that -NR 3 R 4 represents piperidinyl, morpholinyl, pyrrolidinyl, 1,2,3,4-tetrahydroisoquinolinyl or 1-indanyl, or that at least one of R 3 and R 4 represents -(Cti-^)^ or -(CH 2 ) m OA. We particularly prefer that -NR 3 R 4 represents 1,2,3,4-tetrahydroisoquinolinyl or 1-indanyl or that one of R 3 and R 4 represents -(CH 2 ).A and the other represents hydrogen or methyl. We especially prefer that one of R 3 and R 4 represents -(CH 2 ).A and the other represents hydrogen or methyl. When R 2 represents -X(CH 2 )„NHCOR 5 , we prefer that R 5 represents -(CH 2 )Λ When R 2 represents -X(CH 2 ) n ZCONR 3 R 4 , -X(CH 2 )„NHCO(CH 2 ) s NR 3 R 4 , -X(CH 2 ) P NR 3 R 4 or -X(CH 2 ) n NHCOR s , we prefer X to represent a bond. When R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and Z represents NR 7 , we prefer R 7 to represent hydrogen. When R 2 represents -X(CH 2 )„ZCONR 3 R 4 we prefer Z to represent a bond. When R 2 represents -(CH 2 ) q NHC(NH)R 6 , we prefer that R 6 represents phenyl or a 5-membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which two groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6 and halogen. When R 2 represents -(CH 2 ) q NHC(NH)R 6 , we particularly prefer that R 6 represents phenyl or thiophene, which two groups are optionally substituted by one or more groups selected from alkyl Cl to 6 and halogen. When R 2 represents -(CH 2 ) q NHC(NH)R 6 , we prefer that q represents 0, 1 or 2. We particularly prefer that q represents 0 or 2, especially 0. When R 2 represents -(CH 2 ) q NHC(NH)R 6 , q represents 0 and R 6 represents phenyl optionally substituted by halogen, alkyl Cl to 6, or alkoxy Cl to 6 or R 6 represents pyridinyl, then we prefer that D does not have the same definition as R 6 . When R 2 represents -(CH 2 ) q NHC(NH)R 6 and q represents 0, then we generally prefer than R 6 does not have the same definition as D. When R 2 represents -X(CH 2 ) p NR 3 R 4 we prefer that p represents an integer in the range 1 to 4 inclusive, particularly 1, 2 or 3, especially 1 or 2. When R 2 represents -X(CH 2 )„ZCONR 3 R 4 , -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 or -X(CH 2 ) n NHCOR 5 , we prefer n to represent 1, 2 or 3, especially 2 or 3. When R 3 , R 4 or R 5 represent -(CH 2 ).A, we prefer r to represent an integer in the range 0 to 4 inclusive, particularly 0, 1 or 2, more particularly 1 or 2, especially 1. When R 3 or R 4 represent -(CH 2 ) m OA, we prefer m to represent 2, 3 or 4. When R s represents -O(CH 2 ) w A, we prefer w to represent 2, 3 or 4. When R 3 or R 4 represent we prefer t to represent 0, 1 or 2, especially 0 or 1. We prefer that A represents phenyl, pyridinyl, pyrimidinyl, thiophenyl or furanyl, which five groups are optionally substituted by one or more groups selected from alkyl Cl to 6 and halogen. We particularly prefer that A represents phenyl optionally substituted one or more groups selected from alkyl Cl to 6 and halogen. When D or R 5 represent perfluoroalkyl Cl to 6 we prefer that they represent pentafluoroethyl or trifluoromethyl, especially trifluoromethyl. We prefer that R 2 represents -X(CH 2 ) p NR 3 R 4 or -(CH 2 ) q NHC(NH)R 6 . We prefer that the orientation of R 2 is meta or para to the nitrogen atom of the amidine moiety. According to the invention, we further provide a process for the preparation of compounds of formula I, and pharmaceutically acceptable salts thereof, which comprises: (a) preparing a compound of formula I, by reacting a corresponding compound of formula II N H L A, wherein D is as defined above and L is a leaving group, with a compound of formula III wherein R l and R 2 are as defined above, (b) preparing a compound of formula I, by reacting a corresponding compound of formula IV N I V wherein D is as defined above, with a compound of formula V wherein R 1 and R 2 are as defined above and HA is an acid, (c) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 , -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 or -X(CH 2 ) p NR 3 R 4 and at least one of R 3 and R 4 represents alkyl Cl to 6, -(CH 2 ) A -(CH 2 ) ιn OA or -CH(CH 3 )(CH 2 ) t A by reacting a corresponding compound of formula I in which one or both of R 3 and R 4 represents hydrogen with a compound of formula VI, R 8 - L V I wherein R 8 represents alkyl Cl to 6, -(CH 2 ) "( H 2. m 0A or -CH(CH 3 )(CH 2 ) t A and L is a leaving group, (d) preparing a compound of formula I in which R 2 represents -(CH 2 ) q NHC(NH)R 6 by reacting a corresponding compound of formula VII wherein D, R 1 and q are as defined above, with a compound of formula VIII wherein R 6 is as defined above and L is a leaving group, (e) preparing a compound of formula I in which R represents -(CH 2 ) q NHC(NH)R 6 by reacting a corresponding compound of formula IX wherein D, R 1 , q and HA are as defined above, with a compound of formula X N wherein R 6 is as defined above, (f) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 by reacting a corresponding compound of formula XI, wherein D, R 1 , X, n, Z and L are as defined above, with a compound of formula XII, R 3 R NH X I i wherein R 3 and R 4 are as defined above, (g) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 , by reacting a compound of formula XIII wherein D, R\ X and n are as defined above, with a compound of formula XIV 3 R 4 N (C ' H 2 ) s C0L X I V wherein R 3 , R 4 and s are as defined above and L is a leaving group, (h) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n NHCOR s , by reacting a compound of formula XIII with a compound of formula XV R 5 C0L XV wherein R 5 is as defined above and L is a leaving group, (i) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and Z represents NR 7 by reacting a corresponding compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and Z represents -NH with a compound of formula XVI R 7 -L XV I wherein R is as defined above and L is a leaving group, (j) preparing a compound of formula I in which R 2 represents -X(CH 2 ) p NR 3 R 4 , and p is not less than 2, by reduction of a compound of formula XVII wherein D, X, R 1 , R 3 , R 4 and p are as defined above, (k) preparation of a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 and both R 3 and R 4 represent hydrogen, by reduction of a corresponding compound of formula XVHI wherein R 1 , D, p and X are as defined above, (1) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) B ZCONR 3 R 4 , Z represents O or NR 7 and R 3 represents hydrogen by reacting a compound of formula XIX wherein R 1 , D, X and n are as defined above and Z represents O or NR 7 , with a compound of formula XX R - N = C = 0 X X wherein R 4 is as defined above, (m) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) n NHCOR 5 and R 5 represents -O(CH 2 ) . A by reacting a compound of formula XXI O - C - N - ( C H 2 ) X X wherein R 1 , D, X and n are as defined above, with a compound of formula XXII A( CH 2 ) w 0H XX I I wherein A and w are as defined above, (n) preparing a compound of formula I wherein R 2 represents -X(CH 2 )-ZCONR 3 R 4 and Z represents O or NR 7 by reacting a compound of formula XIX with a compound of formula XXIII wherein R 3 and R 4 are as defined above, (o) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , R 3 represents hydrogen and p represents an integer 2 to 5, by reduction of a compound of formula XXIV R -N-CH(CH 2 ) p ., X X ' V wherein R 1 , R 4 , D, X and p are as defined above, (p) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 )_A in which r represents an integer 2 to 6, by reduction of a compound of formula XXV wherein R\ A, D, r and p are as defined above, (q) preparing a compound of formula I wherein R 2 represents - X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents (CH 2 ) m OA, by reduction of a compound of formula XXVI wherein R 1 , A, D, p and m are as defined above, (r) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 ),A in which r represents an integer 2 to 6, by reduction of a compound of formula XXVII wherein R 1 , A, D, p and r are as defined above, or (s) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R\ one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 ) m OA, by reduction of a compound of formula XXVIII A 0 ( C H 2 ' m- 1 C 0 N H ( C H . ) p wherein R 1 , A, D, p and m are as defined above, and where desired or necessary converting the resultant compound of formula I, or another salt thereof, to a pharmaceutically acceptable salt thereof, or vice versa. In process (a), the reaction will take place on stirring a mixture of the reactants in a suitable solvent, for example a lower alkanol e.g. ethanol, isopropanol or tertiary butanol, at a temperature between room temperature and the reflux temperature of the solvent. The reaction time will depend inter alia on the solvent and the nature of the leaving group, and may be up to 48 hours, however it will typically be from 1 to 5 hours. Suitable leaving groups that L may represent include thioalkyl, sulphonic acid, trifluorocarbon sulphonic acid, halide, alkyl and aryl alcohols and tosyl groups; others are recited in 'Advanced Organic Chemistry', J. March (1985) 3rd Edition, McGraw-Hill on page 315 and are well known in the art. In process (b), the reaction is preferably performed by refluxing a mixture of the two compounds for several hours in the presence of a suitable solvent whereby the reaction temperature is high enough so that condensation takes place readily, but not sufficiently high to decompose the amidine formed. The reaction temperature can vary from room temperature to about 250 °C, although it is preferable to perform the reaction at temperatures from about 100 °C to 200 °C. We find that o- dichlorobenzene is a particularly suitable solvent and it is useful to add 4- dimethylaminopyridine as a catalyst. On cooling, two layers form, the solvent may be decanted, and the reaction worked up by addition of aqueous base. Alternatively, where the reactants are soluble in the solvent, the solvent may be evaporated off under vacuum and the reaction mixture worked up by addition of water. The acid HA may be an organic or inorganic acid, for instance, hydrochloric, hydrobromic, hydroiodic, sulphuric, nitric, phosphoric, acetic, lactic, succinic, fumaric, malic, maleic, tartaric, citric, benzoic or methanesulphonic acid. In process (c), the reaction will take place under standard conditions, for example by reacting the two materials in an inert solvent under basic conditions at room temperature for a period of up to 12 hours. We have frequently found it desirable to treat the amine with NaH before reacting with the compound of formula II. We prefer that L represents halide, particularly bromide. Process (d) may be performed under conditions analogous to those described above for process (a). Process (e) may be performed under conditions analogous to those described above for process (b). Processes (f), (g) and (h) may be performed under the standard conditions well known in the art for condensation of an amine and a carboxylic acid or an activated carboxylic acid to form an amide. For example, reaction of compounds to form the amide may be achieved on stirring the reactants for 12-24 hours at a temperature between 0 °C and 25 °C in water or a mixture of water and a less polar solvent, for example dioxan, tetrahydrofuran or ethanol. We prefer to perform the reaction under basic conditions, e.g. in the presence of aqueous sodium carbonate or sodium bicarbonate. Process (i) may be performed under standard conditions analogous to those given above for process (c). In process (j), the reduction may be perfomed by treatment with diborane in an inert solvent e.g. THF. Alternative although less preferred reagents which may be suitable include lithium aluminium hydride and reagents for catalytic hydrogenation e.g. H 2 on Pd/C. Further details of the reaction conditions for use of these reactions may be obtained by reference to J. March "Advanced Organic Chemistry" on page 1099, including the references cited therein. In process (k), the reduction reaction may be performed under a number of conditions, for example those described in J March "Advanced Organic Chemistry" on pages 1103-1104. These include catalytic hydrogenation, use of Zn, Sn or Fe metal, A1H 3 -A1C1 3 , sulphides and others. We prefer to perform the reaction by hydrogenation at atmospheric pressure for 3-6 hours in the presence of a palladium and carbon catalyst. In process (1) and (m), the reaction may be performed by stirring the reactants in the presence of an inert solvent at a temperature between room temperature and the reflux temperature of the solvent for up to 24 hours. Process (n) may be performed under conditions analogous to those described above for processes (f), (g) and (h). In processes (o), (p) and (q), the reduction may be performed by treating the compound with sodium borohydride under standard conditions. In processes (r) and (s), the reaction may be performed under conditions analogous to those described above for process (j). Salts of compounds of formula I may be formed by reacting the free acid, base or a salt, enantiomer, tautomer or protected derivative thereof, with one or more equivalents of the appropriate base or acid. The reaction may be carried out in a solvent or medium in which the salt is insoluble or in a solvent in which the salt is soluble, eg water, dioxan, ethanol, tetrahydrofuran or diethyl ether, or a mixture of solvents, which may be removed in vacuo or by freeze drying. The reaction may be a metathetical process or it may be carried out on an ion exchange resin. It will be apparent to a person skilled in the art that it may be desirable to protect a hydroxy, amine or other reactive group using a protecting group as described in the standard text "Protecting groups in Organic Synthesis", 2nd Edition (1991) by Greene and Wuts. Amine-protecting groups which may be mentioned include alkyloxycarbonyl C2 to 7, eg t-butyloxycarbonyl, phenylalkyloxycarbonyl C8 to 13, eg benzyloxycarbonyl or preferably trifluoroacetate. Deprotection will normally take place on treatment with aqueous base. Compounds of formula II are either known or may be prepared by known methods. For example, compounds of formula II in which L represents thioalkyl may be prepared by treatment of the corresponding thiamide of formula XXIX s I I x x i x "N H . wherein D is as defined above, with an alkyliodide. The compounds of formula III may be prepared by reduction of a corresponding compound of formula XXX wherein R 1 and R 2 are as defined above. The reduction reaction may be performed under analogous conditions to those described above for process (k). Certain compounds of formula XXX are either known or may be prepared by conventional methods known per se. Other compounds of formula XXX may be prepared from known compounds with simpler side-chains by following analogous processes to those described above for processes (c) to (s). Compounds of formula V may be prepared by analogous processes to those described for the preparation of compounds of formula III. Compounds of formula V may be converted to corresponding compounds of formula III by treatment with a base. Compounds of formula HI may be converted to corresponding compounds of formula V by treatment with a protic acid HA, for example one of those listed above. Compounds of formula VH, IX, XI, Xϋl, XVII, XVIII, XIX, XXI, XXIV, XXV, XXVI, XXVII and XXVIII may be prepared by analogous processes to those described for the preparation of compounds of formula I. Compounds of formula VIII are either known or may be prepared by an analogous process to that described above for preparation of compounds of formula π. Compounds of formula IV, VI, X, XII, XVI, XX, XXII, XXIII and XXIX are either known or may be prepared by conventional methods known per se. Compounds of formula XTV and XV are either known or may readily be prepared from the corresponding carboxylic acid which is either known or may be prepared by conventional methods known per se. Where necessary, hydroxy, amine or other reactive groups in intermediate compounds may be protected using a protecting group as described in the standard text "Protecting groups in Organic Synthesis", 2nd Edition (1991) by Greene and Wuts. The compounds of the invention and intermediates may be isolated from their reaction mixtures by standard techniques. The term "alkyl Cl to 6" includes straight chain, branched, saturated, unsaturated, aliphatic and cyclic alkyl groups containing 1 to 6 carbon atoms. The compounds of formula I may exist in tautomeric, enantiomeric or diasteriomeric forms, all of which are included within the scope of the invention. The various optical isomers may be isolated by separation of a racemic mixture of the compounds using conventional techniques, e.g. fractional crystallisation, or HPLC. Alternatively the individual enantiomers may be made by reaction of the appropriate optically active starting materials under reaction conditions which will not cause racemisation. Intermediate compounds may also exist in enantiomeric forms and may be used as purified enantiomers, diastereomers, racemates or mixtures. The compounds of general formula I possess useful pharmacological activity in animals. In particular, they possess useful nitric oxide synthetase inhibiting activity, and are expected to be useful in the treatment or prophylaxis of human diseases or conditions in which the synthesis or oversynthesis of nitric oxide forms a contributory part; for example, hypoxia, e.g. in cases of cardiac arrest and stroke, neurodegenerative disorders including nerve degeneration and/or nerve necrosis in disorders such as hypoxia, hypoglycemia, epilepsy, and in external wounds (such as spinal cord and head injury), hyperbaric oxygen convulsions and toxicity, dementia e.g. pre-senile dementia, Alzheimer's disease and AIDS-related dementia, Sydenham's chorea, Parkinson's disease, Huntington's disease, Amyotrophic Lateral Sclerosis, Korsakoffs disease, imbecility relating to a cerebral vessel disorder, sleeping disorders, schizophrenia, depression, seasonal affective disorder, jet-lag, depression or other symptoms associated with Premenstrual Syndrome (PMS), anxiety and septic shock. Compounds of formula I may also be expected to show activity in the prevention and reversal of tolerance to opiates and diazepines, treatment of drug addiction, relief of pain and treatment of migraine and other vascular headaches. The compounds of the present invention may also show useful immunosuppressive activity, be useful in the treatment or prophylaxis of inflammation, in the treatment of of gastrointestinal motility disorders, and in the induction of labour. Compounds of formula I are expected to be particularly useful in the treatment of neurodegenerative disorders or of migraine or for the prevention and reversal of tolerance to opiates and diazepines or for the treatment of drug addiction and especially in the treatment of neurodegenerative disorders. Thus according to a further aspect of the invention we provide a compound of formula I, or a pharmaceutically acceptable salt thereof, for use as a pharmaceutical. According to another feature of the invention we provide the use of a compound of formula I, without provisos (e) to (h), or a pharmaceutically -Un¬ acceptable salt thereof, in the manufacture of a medicament for the treatment of the aforementioned diseases or conditions. For the above mentioned therapeutic indications, the dosage administered will, of course, vary with the compound employed, the mode of administration and the treatment desired. However, in general, satisfactory results are obtained when the compounds are administered to a human at a daily dosage of between 1 mg and 2000 mg (measured as the solid form) per day. The compounds of formula I, and pharmaceutically acceptable salts thereof, may be used on their own, or in the form of appropriate medicinal preparations for enteral or parenteral administration. According to the invention, there is provided a pharmaceutical formulation including preferably less than 80% and more preferably less than 50% of a compound of formula I, or a pharmaceutically acceptable salt thereof, in admixture with a pharmaceutically acceptable diluent or carrier. There is also provided a method of treatment of one of the aforementioned diseases or conditions which comprises administering a therapeutically effective amount of a compound of formula I, without provisos (e) to (h), or a pharmaceutically acceptable salt thereof, to a person suffering from such a disease or condition. Examples of such diluents and carriers are: for tablets and dragees: lactose, starch, talc, stearic acid; for capsules: tartaric acid or lactose; for injectable solutions: water, alcohols, glycerin, vegetable oils; for suppositories: natural or hardened oils or waxes. Compositions in a form suitable for oral, i.e. oesophageal administration include: tablets, capsules and dragees; sustained release compositions include those in which the active ingredient is bound to an ion exchange resin which is optionally coated with a diffusion barrier to modify the release properties of the resin. The enzyme nitric oxide synthetase has a number of isoforms and compounds of formula I, or pharmaceutically acceptable salts thereof, may be screened for nitric oxide synthetase activity by procedures based on those of Bredt and Snyder in Proc. Natl. Acad. Sci. (1990) 87, 682-685 and Fδrstermann et. al. (1992) Eur. J. Pharm. 225,161-165 as follows. Nitric oxide synthetase converts 3 H-L-arginine to 3 H-L- citrulline which can be separated by cation exchange chromatography and quantified by liquid scintillation counting. Screen A (A) Screen for neuronal nitric oxide synthetase activity Enzyme was isolated from rat hippocampus or cerebellum. The cerebellum or hippocampus of a male Sprague-Dawley rat (250-275g) is removed following CO 2 anaesthesia of the animal and decapitation. Cerebellar or hippocampal supernatant is prepared by homogenisation in 50 mM Tris-HCl with 1 mM EDTA buffer (pH 7.2 at 25 °C) and centifugation for 15 minutes at 20,000 g. Residual L-arginine is removed from the supernatant by chromatography through Dowex AG-50W-X8 sodium form and hydrogen form columns successively, and further centrifugation at 1000 g for 30 seconds. For the assay, 25 μl of the final supernatant is added to each of 12 test tubes containing 25 μl L-arginine solution (of concentration 18 μM x H-L-arginine, 96 nM 3 H-L-arginine) and either 25 μl of an assay buffer (50 mM HEPES, 1 mM EDTA, 1.5 mM CaCl-j, pH 7.4) or 25 μl of test compound in the buffer at 22 °C. To each test tube was added 75 μl of complete assay buffer (50 mM HEPES, 1 mM EDTA, 1.5 mM CaCl 2 , 1 mM DTT, 100 μM NADPH, 10 μg/ml calmodulin, pH 7.4) to initiate the reaction and the reaction is stopped after 10 minutes by addition of 2 ml of a termination buffer (20 mM HEPES, 2 mM EDTA pH 5.5). Labelled L-citrulline is separated from labelled L-arginine by chromatography over a Dowex AG-50W-X8 200-400 mesh column. 1 ml of each terminated reaction is added to an individual 1 ml column and the eluant combined with that from two 1 ml distilled water washes and 16 ml of scintillation cocktail. The L- citrulline is then quantified by scintillation counting. In a typical experiment using the cerebellar supernatant, basal activity is increased by 20,000 dpm ml of sample above a reagent blank which has an activity of 7,000 dpm/ml. A reference standard, N-nitro-L-arginine, which gives 60% inhibition of nitric oxide synthetase at a concentration of 1 μM, is tested in the assay to verify the procedure. Screen B (B) Screen for macrophage nitric oxide synthetase activity Enzyme is prepared, after induction, from the cultured murine macrophage cell line J774A-1 (obtained from laboratories of the Imperial Cancer Research Fund). J774A-1 cells are cultured in Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% foetal bovine serum, 4 mM L-glutamine and antibiotics (100 units/ml penicillin G, 100 μg/ml streptomycin & 0.25 μg/ml amphotericin B). Cells are routinely grown in 225 cm 2 flasks containing 35 ml medium kept at 37 °C and in a humidified atmosphere containing 5% CO 2 . Nitric oxide synthetase is produced by cells in response to interferon-γ (IFN7) and lipopolysaccharide (LPS). The medium from confluent culture flasks is removed and replaced with 25 ml (per flask) of fresh medium containing 1 μg/ml LPS and 10 units/ml IFN7. After a period of 17-20 hours in culture, harvesting of cells is accomplished by scraping the cell sheet from the flask surface into the culture medium. Cells are collected by centrifugation (lOOOg for 10 minutes) and lysate prepared by adding to the cell pellet a solution containing 50 mM Tris-HQ (pH 7.5 at 20 °C), 10% (v/v) glycerol, 0.1% (v/v) Triton-X-100, 0.1 μM dithiothreitol and a cocktail of protease inhibitors comprising leupeptin (2 μg/ml), soya bean trypsin inhibitor (10 μg/ml), aprotinin (5 μg/ml) & phenylmethylsulphonyl fluoride (50 μg/ml). For the assay, 25 μl substrate cocktail (50 mM Tris-HCl (pH 7.5 at 20 °C), 400 μM NADPH, 20 μM flavin adenine dinucleotide, 20 μM flavin mononucleotide, 4 μM tetrahydrobiopterin, 12 μM L-arginine and 0.025 μCi L-[ 3 H] arginine) is added to wells of a 96 well filter plate (0.45μM pore size) containing 25 μl of a solution of test compound in 50 mM Tris-HQ. The reaction is started by adding 50 μl of cell lysate (prepared as above) and after incubation for 1 hour at room temperature is terminated by addition of 50 μl of an aqueous solution of 3 mM nitroarginine and 21 mM EDTA. Labelled L-citrulline is separated from labelled L-arginine using Dowex AG- 50W. 150 μl of a 25% aqueous sluπy of Dowex 50W (Na + form) is added to the assay after which the whole is filtered into 96 well plates. 70 μl of filtrate is sampled and added to wells of 96 well plates containing solid scintillant. After allowing the samples to dry the L-citrulline is quantified by scintillation counting. In a typical experiment basal activity is 300 dpm per 70 μl sample which is increased to 1900 dpm in the reagent controls. Aminoguanidine, which gives an IC 50 (50% inhibitory concentration) of 10 μM, is tested as a standard to verify the procedure. Screen C (C) Screen for endothelial nitric oxide synthetase activity Enzyme may be isolated from human umbilical vein endothelial cells (HUVECs) by a procedure based on that of Pollock et al (1991) Proc. Nat. Acad. Sci., 88, 10480-10484. HUVECs were purchased from Clonetics Corp (San Diego, CA, USA) and cultured to confluency. Cells can be maintained to passage 35-40 without significant loss of yield of nitric oxide synthetase. When cells reach confluency, they are resuspended in Dulbecco's phosphate buffered saline, centrifuged at 800 rpm for 10 mins, the cell pellet homogenised in ice-cold 50 mM Tris-HQ, 1 mM EDTA, 10% glycerol, 1 mM phenylmethylsulphonylfluoride, 2 μM leupeptin at pH 4.2. Following centrifugation at 34,000 rpm for 60 mins, the pellet is solubilised in the homogenisation buffer which also contains 20 mM CHAPS. After a 30 min incubation on ice, the suspension is centrifuged at 34,000 rpm for 30 mins. The resulting supernatant is stored at -80 °C until use. For the assay, 25 μl of the final supernatant is added to each of 12 test tubes containing 25 μl L-arginine solution (of concentration 12 μM 'H-L-arginine, 64 nM 3 H-L-arginine) and either 25 μl of an assay buffer (50 mM HEPES, 1 mM EDTA, ( 1.5 mM CaCl 2 , pH 7.4) or 25 μl of test compound in the buffer at 22 °C. To each test tube was added 25 μl of complete assay buffer (50 mM HEPES, 1 mM EDTA, 1.5 mM CaCl 2 , 1 mM DTT, 100 μM NADPH, 10 μg/ml calmodulin, 12 μM tetrahydrobiopterin, pH 7.4) to initiate the reaction and the reaction is stopped after 10 mins by addition of 2 ml of a termination buffer (20 mM HEPES, 2 mM EDTA, pH 5.5). Labelled L-citrulline is separated from labelled L-arginine by chromatography over a Dowex AG-50W-X8 200-400 mesh column. 1 ml of each terminated reaction is added to an individual 1 ml column and the eluant combined with that from two 1 ml distilled water washes and 16 ml of scintillation cocktail. The L- citrulline is then quantified by scintillation counting. In a typical experiment, basal activity is increased by 5,000 dpm/ml of sample above a reagent blank which has an activity of 1500 dpm/ml. A reference standard, N-nitro-L-arginine, which gives 70-90% inhibition of nitric oxide synthetase at a concentration of 1 μM, is tested in the assay to verify the procedure. Compounds may also be tested in an ex-vivo assay to determine the extent of brain penetration. Screen D (D) Ex vivo assay for neuronal nitric oxide synthetase activity Male Sprague-Dawley rats (250-275g) were dosed intravenously at lOmg/kg with test compound dissolved in 0.9% saline or with saline alone as control. At a predetermined time (typically 2-24 hours) after treatment, the animals were sacrificed, the cerebellum removed and the supernatant prepared and assayed for nitric oxide synthetase activity as described in Screen A As a further confirmatory test, a fraction of the cerebellar supernatant was applied to a 2'-5'-ADP Sepharose column (which binds nitric oxide synthetase) and subsequently eluted with NADPH. The eluant was tested for nitric oxide synthetase activity following the procedure of Screen A. Compounds that penetrate the rat brain and inhibit neuronal nitric oxide synthetase resulted in reduced nitric oxide synthetase activity both in the supernatant preparation and in the eluant from the 2'-5'-ADP Sepharose column. In the screens for nitric oxide synthetase inhibition activity, compound activity is expressed as IC^ (the concentration of drug substance which gives 50% enzyme inhibition in the assay). IC S0 values for test compounds were initially estimated from the inhibiting activity of 1, 10 and 100 μM solutions of the compounds. Compounds that inhibited the enzyme by at least 50% at 10 μM were retested using more appropriate concentrations so that an IC 50 could be determined. In Screen A above (a screen for activity against the neuronal isoform of nitric oxide synthetase), the compound of Example 1 below gave an IC 50 of less than 10 μM indicating that it is expected to show useful therapeutic activity. In Screens B and C (the screens for activity against the macrophage and endothelial isoforms of nitric oxide synthetase) the compound of Example 1 gave IQ,, values more than 10 times that obtained in Screen A indicating that it shows desirable selectivity. The compounds of Examples 2-20, 21(a)-(n), 22(a)-(e), 23(a)-(f), 24-26, 27(a), (b), 28-47 and 49-71 were tested in Screen A and also gave IC 50 values of less than 10 μM. Example 48 was tested in Screen A and gave an IC S0 value of less than 100 μM. Example 72 was tested in Screen A and gave 17% inhibition at 10 μM. Thus these compounds are also expected to show useful therapeutic activity. Compounds of formula I, and pharmaceutically acceptable salts thereof, have the advantage that they are less toxic, more efficacious, more selective, are longer acting, have a broader range of activity, are more potent, produce fewer side effects, are more easily absorbed, or have other useful pharmacological properties than compounds previously known and used in the therapeutic fields mentioned above. Compounds of formula I, and pharmaceutically acceptable salts thereof, may also have the advantage that they are more selective for the neuronal isoform of nitric oxide synthetase enzyme and are therefore expected to show useful therapeutic activity with a reduced side-effect profile associated with inhibition of the other isoforms. The invention is illustrated by the following examples: Example 1 N- 4-f2- phenylmethyl > )amino < )ethyl )phenylV2-thiophenecarboximidamide (a) N-.2-f4-nitrophenvnethvDtrifluoroacetan.ide To a stirred solution of 4-nitrophenethylamine hydrochloride (1.84 g, 9.10 mmol) and triethylamine (3.03 ml, 21.70 mmol) in methanol (12 ml) was added trifluoroacetic anhydride (1.51 ml, 10.66 mmol) dropwise. After stirring for 1 minute, the solvent was removed at reduced pressure and the remaining residue was mixed with water and extracted with methylene chloride (3 X 20 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to yield a solid which was recrystallized from methylene chloride/hexane to give N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide as a white solid: 1.92 g (80% yield); m.p. 103-104 °C. (b) N- 2-f4-nitrophenyl .ethylVN- phenylmethvπtrifluoroacetamide To a stirred solution of the product of step (a) (0.89 g, 3.40 mmol) in THF (5 ml) at 0 °C was added NaH (60%, 0.18 g, 4.42 mmol) followed by benzyl bromide (0.50 ml, 4.10 mmol). The mixture was stirred at room temperature for 6 h, quenched with water, and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated, and chromatographed over silica gel (18% ethylacetate/hexane) to give N(2-(4- nitrophenyl)ethyl)-N-(phenylmethyl)trifluoroacetamide as a colourless oil: (0.52 g, 44%); M.S. (M+H) + = 353. (c) N-, 2-f4-aminophenvDethylVN-,phenylmethvDtrifluoroacetamide To a stirred solution of the product of step (b) (0.52 g, 1.48 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give N(2-(4-aminophenyl)ethyl)-N-(phenylmethyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction. (d) S-methyl-2-thiophenethiocarboximide hydroiodide A solution of 2-thiophenecarboxthioamide (Maybridge Chemical) (11.1 g) in 60ml of acetone was treated with iodomethane (13.4g). After 6 hrs at 22 °C, the resulting yellow solids were collected by filtration, washed twice with 25ml of acetone and dried to provide 18.45 g of S-methyl-2-thiophenethiocarboximide hydroiodide, m.p.195 °C (dec). (e) N- 4-C2-r phenylmethyl " .amino',ethvnphenylV2-thiophenecarboximidamide To a solution of N(2-(4-aminophenyl)ethyl)-N-(phenylmethyl)trifluoroacetamide (0.48 g, 1.48 mmol) in isopropanol (6 ml) was added S-methyl-2- thiophenethiocarboximide hydroiodide (0.42 g, 1.48 mmol). The mixture was stirred for 4 hr, diluted with methanol (5 ml) and 2 N NaOH (6 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to give a solid which was recrystallized (ethyl acetate/hexane) to yield N-(4-(2-((phenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (0.17 g, 34%); m.p. 116-118 °C. Example 2 N- 4- i-f phenylmethyl )amino > )methyl )phenylV2-thiophenecarboximidamide (a) N-ff4-nitrophenyDmethyl')trifluoroacetamide To a stirred solution of 4-nitrobenzylamine hydrochloride (4.06 g, 21.5 mmol) and triethylamine (6.60 ml, 47.4 mmol) in methylene chloride (30 ml) was added trifluoroacetic anhydride (3.34 ml, 23.7 mmol) dropwise. After stirring for 1 minute, water was added and the layers separated. The aqueous layer was further extracted with methylene chloride (3 X 20 ml) and the combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to yield a solid which was re crystallized from methylene chloride/hexane to give N-((4- nitrophenyl)methyl)trifluoroacetamide as a white solid: 3.9 g (73% yield); m.p. 97- 98 °C. .b . N-, , 4-nitrophenvπmethviyN-, phenylmethvntrifluoroacetamide To a stirred solution of the product of step (a) (1.0 g, 4.03 mmol) in THF (10 ml) at 0 °C was added NaH (60%, 0.21 g, 5.24 mmol) followed by benzyl bromide (0.72 ml, 4.84 mmol). The mixture was stirred at room temperature for 12 h, quenched with water, and extracted with ethyl acetate( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated, and chromatographed over silica gel (16% ethylacetate/hexane) to give N-((4- nitrophenyl)methyl)-N-(phenylmethyl)trifluoroacetamide as a colorless oil: (0.50 g, 40%); M.S. (M+H) + = 339. (c) N(Y4-aminophenvnmethviyN-, phenylmethyπtrifluoroacetamide To a stirred solution of the product of step (b) (1.76 g, 5.16 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 0.5 hr, filtered through celite, and concentrated to give N((4-aminophenyl)methyl)-N-(phenylmethyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction. (d) N. -4-. l- phenylmethvnamino )methvnphenylV2-thiophenecarboximidamide To a solution of the product of step (c) (1.60 g, 5.16 mmol) in isopropanol (6 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.47 g, 5.16 mmol). The mixture was stirred for 24 hr at 40 °C, diluted with methanol (5 ml) and 2 N NaOH (15 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated and chromatographed over silica gel (8% methanol/ methylene chloride) to give a solid which was recrystallized (ethyl acetate/hexane) to yield N-(4-(l- ((phenylmethyl)amino)methyl)phenyl)-2-thiophenecarboximidamide as a white solid: (60 mg, 4%); m.p. 73-74 °C. Example 3 N-^Cl- Cphenethvnamino^methvnphenvn^-thiophenecarboximidamide (a) N-f2-phenylethyntrifluoroacetamide To a stirred solution of phenethylamine (4.91 g, 40.5 mmol) and triethylamine (6.50 ml, 46.6 mmol) in methylene chloride (30 ml) was added trifluoroacetic anhydride (6.3 ml, 44.6 mmol) dropwise. After stirring for 1 minute, water was added and the layers separated. The aqueous layer was further extracted with methylene chloride (3 X 40 ml) and the combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to yield a solid which was recrystallized from methylene chloride/hexane to give N-(2- phenylethyl)trifluoroacetamide as a white solid: 6.0 g 69% yield); m.p. 50-52 °C. (b) N-f2-phenylethylVN-, f4-nitrophenvDmethvntrifluoroacetamide To a stirred solution of the product of step (a) (2.0 g, 9.26 mmol) in THF (10 ml) at 0 °C was added NaH (60%, 0.37 g, 9.26 mmol) followed by 4-nitrobenzyl bromide (1.0 g, 4.63 mmol). The mixture was stirred at room temperature for 1 hr, quenched with water, and extracted with ethyl acetate (3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated, and chromatographed over silica gel (16% ethylacetate/hexane) to give N-(2- phenylethyl)-N-((4-nitrophenyl)methyl)trifluoroacetamide as a colorless oil: (1.60 g, 98%); M.S. (M+H) + = 353. (c) N-f2-phenylethyl>N-, f4-aminophenvDmethyl ,trifluoroacetamide To a stirred solution of the product of step (b) (1.60 g, 4.54 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 0.75 hr, filtered through celite, and concentrated to give N-(2-phenylethyl)-N-((4-aminophenyl)methyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction. (d) N- 4- i- 2-phenylethvπamino )methyl lphenylV2-thiophenecarboximidamide To a solution of the product of step (c) (1.47 g,4.54 mmol) in isopropanol (5 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.30 g,4.54 mmol). The mixture was stirred for 24 hr at 40 °C, diluted with methanol (5 ml) and 2 N NaOH (10 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated and chromatographed over silica gel (10% methanol/methylene chloride) to give a solid which was recrystallized (ethyl acetate/hexane) to yield N-(4-(l-((2- phenylethyl)amino)methyl)phenyl)-2-thiophenecarboximidamide as a white solid: (20 mg, 2%); M.S. (M+H) + = 336. Example 4 N-C4-('2-f 2-chlorophenylmethyl ' .amino')ethyl ,phenylV2-thiophenecarboximidamide (a) N-, 4-nitrophenvDethylVN-ff2-chlorophenyl^methvntrifluoroacetamide To a stirred solution of N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide (the product of Example 1 step (a)) (2.0 g, 7.63 mmol) and a catalytic amount of 15-crown-5 in THF (10 ml) at 0 °C was added NaH (60%, 0.18 g, 4.42 mmol) followed by 2- chlorobenzyl bromide (1.49 ml, 11.45 mmol). The mixture was stirred at room temperature for 2 hr, quenched with water, and extracted with ethyl acetate( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated, and chromatographed over silica gel (18% ethylacetate/hexane) to give N-(2-(4-nitrophenyl)ethyl)-N-((2- chlorophenyl)methyl)trifluoroacetamide as a colourless oil: (2.31 g, 78%); M.S. (M+H) + = 353. (b) N- 2- 4-aminophenyl")ethylVN-f 2-chlorophenyl ,methyl )trifluoroacetamide To a stirred solution ofthe product of step (a) (2.31 g, 5.96 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give N- (2-(4-aminophenyl)ethyl)-N-((2-chlorophenyl)methyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction. (c) N-f4-, 2- 2-chlorophenylmethyl )amino ' .ethyl'iphenylV2- thiophenecarboximidamide To a solution of the product of step (b) (2.1 g, 5.96 mmol) in isopropanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.7 g, 5.96 mmol). The mixture was stirred for 24 hr, diluted with methanol (10 ml) and 2 N NaOH (6 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated and chromatographed over silica gel (10% methanol/methylene chloride) to give a solid which was recrystallized (methylene chloride/hexane) to yield N-(4-(2-((2- chlorophenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (0.21 g, 10%); m.p. 81-82 °C. Example 5 N- 4- 2- r3-fluorophenylmethyl )amino")ethyl )phenylV2-thiophenecarboximidamide (a) N-rr3-fluorophenyl , methyl ' .-N-f2-r4-nitrophenyl ' ,ethyl > )trifluoroacetamide To a stirred solution of N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide (the product of Example 1 step (a)) (1.5 g, 5.75 mmol) and a catalytic amount of 15-crown-5 in THF (10 ml) at 0 °C was added NaH (60%, 0.25 g, 6.34 mmol) followed by 3- fluorobenzyl bromide (1.40 ml, 11.45 mmol). The mixture was stirred at room temperature for 4 hr, quenched with water, and extracted with ethyl acetate( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated, and chromatographed over silica gel (18% ethyl acetate/hexane) to give N-((3-fluorophenyl)methyl)-N-(2-(4- nitrophenyl)ethyl)trifluoroacetamide as a colourless oil: (1.63 g, 77%); M.S. (M+H) + = 371. (b) N-r2- 4-aminophenyl)ethylVN- r3-fluorophenvnmethvπtrifluoroacetamide To a stirred solution of the product of step (a) (1.63 g, 4.40 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give N- (2-(4-aminophenyl)ethyl)-N-((3-fluorophenyl)methyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction, (c) N- 4-r2- r3-fluoroDhenylmethvDamino .ethyl1phenylV2- thiophenecarboximidamide To a solution of the product of step (b) (1.5 g, 4.40 mmol) in methanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.3 g, 4.40 mmol). The mixture was stirred for 2 hr, diluted with methanol (5 ml) and 2 N NaOH (8 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to give a solid which was recrystallized (methylene chloride/hexane) to yield N-(4-(2-(((3- fluorophenyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (0.14 g, 8%); m.p.130-131 °C. Example 6 N-f4-r2-fff2-methylDhenyl .methyl .amino)ethyl .phenyl ' .-2- thiophenecarboximidamide (a) N-. .2-methylphenv0methylVN-f2-f4-nitrophenvDethv0trifluoroacetamide To a stirred solution of N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide (the product of Example 1 step (a)) (1.5 g, 5.75 mmol) and a catalytic amount of 15-crown-5 in THF (10 ml) at 0 °C was added NaH (60%, 0.25 g, 6.34 mmol) followed by 2- methylbenzyl bromide (1.53 ml, 11.45 mmol). The mixture was stirred at room temperature for 2 hr, quenched with water, and extracted with ethyl acetate( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated, and chromatographed over silica gel (18% ethylacetate/hexane) to give N-((2-methylphenyl)methyl)-N-(2-(4- nitrophenyl)ethyl)trifluoroacetamide as a colorless oil: (1.76 g, 84%); M.S. (M+H) + = 367. (b)N-f2- 4-aminophenvnethylVN-r 2-methylphenvπmethylVtrifluoroacetamide To a stirred solution of the product of step (a) (1.76 g, 4.82 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give N- (2-(4-an___nophenyl)ethyl)-N-((2-methylpheπyl)methyl)-trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction, (c) N- 4-f2- rr2-methylDhenyl .methvnammo' ethvnphenylV2- thiophenecarboximidamide To a solution of the product of step (b) (1.62 g, 4.82 mmol) in methanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.37 g, 4.82 mmol). The mixture was stirred for 2 hr, diluted with 2 N NaOH (8 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to give a solid which was recrystallized (methylene chloride/hexane) to yield N-(4-(2-(((2- methylphenyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (0.46 g, 28%); m.p.105-106 °C. Example 7 N-f4-f2-fmethylamino ' .ethyl )phenviy2-thiophenecarboximidamide (a) N-methyl-N-, 2-r4-nitrophenyl .ethvDtrifluoroacetamide To a stirred solution of N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide (the product of Example 1 step (a)) (1.5 g, 5.75 mmol) and a catalytic amount of 15-crown-5 in THF (10 ml) at 0 °C was added NaH (60%, 0.25 g, 6.34 mmol) followed by methyhodide (0.71 ml, 11.45 mmol). The mixture was stirred at room temperature for 4 hr, quenched with water, and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to yield N-methyl-N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide a colorless oil: (1.40 g, 88%); M.S. (M+H) + = 277. (b) N-methyl-N-f2-f4-aminophenvDethv0trifluoroacetamide To a stirred solution of the product of step (a) (1.45 g, 5.25 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give N- methyl-N-(2-(4-aminophenyl)ethyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction. (c) N-.4-, 2-Cmethylamino')ethvnphenylV2-thiophenecarboximidamide To a solution of the product of step (b) (1.32 g, 5.37 mmol) in methanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.53 g, 5.37 mmol). The mixture was stirred for 2 hr, diluted with 2 N NaOH (8 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to give a solid which was recrystallized (methylene chloride/hexane) to yield N-(4-(2-((2- methylphenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (0.43 g, 31%); M.S. (M+H) + = 260. Example 8 N-.4-f2-aminoethvDphenviy2-thiophenecarboximidamide (a) N-f2-f4-aminophenyl , )ethyl ' Urifluoroacetamide To a stirred solution of N-(2-(4-nitrophenyl)ethyl)trifluoroacetamide (the product of Example 1 step (a)) (1.00 g, 3.81 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give N-(2-(4- aminophenyl)ethyl)trifluoroacetamide which was homogeneous by TLC and used immediately in the next reaction. (c) N-.4-f2-aminoethyl1phenyl>2-thiophenecarboximidamide To a solution of the product of step (a) (0.88 g,3.81 mmol) in methanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.09 g, 3.81 mmol). The mixture was stirred for 12 hr, diluted with 2 N NaOH (8 ml) and heated to 70 °C for 1 hr. The solvents were removed at reduced pressure, and the residue was dumped into water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to give a solid which was recrystallized (ethyl acetate/methanol) to yield N-(4-(2-aminoethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (70 m g, 8%); m.p.134-137 °C. Example 9 N-. .4-mo holinylmethyl > )phenyl>2-thiophenecarboximidan.ide (a) 4-, 4-NitrobenzylVmorpholine. To a stirred solution of (2.00g; 0.0093 mol) 4-nitrobenzyl bromide (Aldrich) and (0.736g; 0.011 mol) potassium carbonate anhydrous (Aldrich) in 20.0ml DMF was added (0.796ml; 0.0093 mol) morpholine. The reaction was heated to 50°C and stirred for 30 minutes, after which time and additional 0.1 equivalents of morpholine and potassium carbonate were added. After 30 minutes the reaction mixture was quenched with 100 ml water and extracted with (4x100ml) ethyl acetate. The organic layers were collected and dried over magnesium sulfate and the solvent evaporated. The resulting solids were recrystallized from ethly acetate and hexane to leave 1.90g of 4-(4-nitrobenzyl)-morpholine. (b) r4-morpholinylmethyl , )aniline A (l.OOg; 0.0045 mol) sample of 4-(4-nitrobenzyl)-morpholine was dissolved in 25ml each THF and methanol in a pressure bottle. A catalytic amount of 10% palladium on carbon was added and the reaction hydrogenated. When hydrogen uptake had ceased, the catalyst was removed by filtration and the solvents evaporated. The solids were dissolved in 30ml each ethyl acetate and water, and 30 ml 2N sodium hydroxide. The aqueous layer was extracted with (4x75ml) ethyl acetate. The organic layers were collected, dried, over magnesium sulfate, and the solvent evaporated under vacuum. The resulting solids were recrystallized from ethyl acetate and hexane to leave 0.68g of (4-morpholinylmethyl)aniline. (c) N- r4-morpholinylmethyl ' .phenylV2-thiophenecarboximidamide To a stirred solution of the product of step (b) (0.68g; 0.0035 mol) and 15.0ml isopropyl alcohol was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (0.99g; 0.0035 mol). The mixture was stirred at 35C. To this mixture was added 10.0ml methanol along with 2M hydrochloric acid in isopropyl alcohol added dropwise until all of the reactants were in solution. The reaction was allowed to stir for 48 hours. The reaction was then diluted in 50ml saturated sodium chloride and extracted with (3x75mL) ethyl acetate. The organic layers were collected, dried over magnesium sulfate, and the solvent evaporated. The crude product was separated on a silica gel column eluted with 10% methanol in methylene chloride. The solvent was evaporated and the crude solid recrystallized twice from ethyl acetate and hexane to leave 60mg of N-((4- morpholinylmethyl)phenyl)-2-thiophenecarboximidamide, m.p. = 148-150°C. Example 10 N-( ' 3-. r phenylmethyl .amino .methyl )phenylV2-thiophenecarboximidamide bisoxalate (a) N-.3-nitrobenzvDbenzamide To a solution of 3-nitrobenzyl amine hydrochloride (2.45 g, 0.013 mol) in a solution of 50 ml of methylene chloride and 50 ml of half saturated aqueous potassium carbonate at 0 °C was added dropwise a solution of benzoyl chloride (2.1 g, 0.0149 mol) in 10 ml of methylene chloride. After addition was complete, the reaction mixture was stirred for 2 h at 0 °C and was then allowed to warm to ambience overnight. The organic layer was separated and was washed successively with dilute hydrochloric acid and water. The dried (MgSO 4 ) organic phase was concentrated in vacuo to give 2.92 g (88%) of the title product, m.p. 136-8°C. (b) N-benzyl-2.2.2-trifluoro-N-f3-nitrobenzvDacetamide To a solution of the product of step (a) (2.85 g, 11.1 mmol) in 50 ml of anhydrous tetrahydrofuran at 0 °C under nitrogen was added 18.6 ml of a 1.0 M borane in THF solution (18.6 mmol). The reaction mixture was then heated to reflux for 5.5 h. The solution was allowed to cool overnight and was then quenched by the successive addition of 2 ml of methanol and 10 ml of 6 M hydrochloric acid. The reaction mixture was again heated to reflux for 1 h. Upon cooling to ambience, the reaction mixture was basified and extracted into ether. The dried (MgSO 4 ) was concentrated to afford an oil. This oil was chromatographed on silica gel using methylene chloride as eluent to give 1.95 g (72 %) of benzyl-3-(nitrobenzyl)amine as an oil. To a solution of the crude be____yl-3-(nitrobeι___yl)amine (1.95 g, 8.05 mmol) and triethylamine (2.6 Ml, 18 mmol) in 20 ml of methylene chloride under nitrogen at Oo C, was added dropwise triflluoroacetic anhydride (3.4 g, 16 mmol). The reaction mixture was stirred for 10 minutes before pouring into water. The organic layer was separated and dried over magnesium sulfate. The solution was filtered and concentrated to give an oil. Chromatography on silica gel, using 20% ethyl acetate in hexanes as eluent afforded 1.46 g (54%) of the product as an oil, mass spectra m/e 339 (100%, M+H). (c) / N- 3-aminobenzylVN-benzyl-2.2.2-trifluoroacetamide To a solution of the product of step (b) (1.21 g, 3.58 mmol) dissolved in a solution of 100 ml of methanol was added 20 ml of a saturated solution of hydrogen chloride in isopropanol and 0.1 g of 5% Pd/C. The resulting solution was hydrogenated at 50 psi for one hour. The catalyst was removed by filtration and the filtrate was concentrated in vacuo sohd. Trituration of this solid with ether afforded 1.15 g (93%) of the title compound as the hydrochloride salt, m.p. 169-74 °C. (d) N-f3-(Tfphenylmethyl )amino)methyl)phenylV2-thiophenecarboximidamide To a solution of 0.25 g (0.94 mmol) of S-methyl 2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) in 4 ml of isopropanol was added 0.41 g (1.3 mmol) of N-(3-aminoben__yl)-N-benzyl-2,2,2-trifluoroacetamide (prepared by taking the hydrochloride salt and neutralizing with 2.5 m NaOH and extracting into methylene chloride). The reaction mixture was stirred for 5 h. A solution (2 ml) of 2.5 M sodium hydroxide and about 5 drops of methanol was added and the resulting solution was heated at reflux for 1 h. The solution was concentrated and the product was extracted into ethyl acetate. The solution was dried and concentrated to give a solid. This solid was dissolved in ethanol and oxalic acid dihydrate (0.16 g, 1.3 mmol) was added. The resulting salt was collected and dried to give 0.26 g (55%) of the title compound as the bis oxalate salt, mp 178-183 °C. Example 11 An alternative synthesis for the compound of Example 2. N-.4- r phenylmethvnamino)methyl .phenylV2-thiophenecarboximidamide (a) N-.4-nitrobenzvDbenzamide . This compound was prepared following the method of Example 10, step (a). From 4-nitroben__yl amine (2.45 g, 0.013 mol) and benzoyl chloride (2.1 g, 0.0149 mol) was isolated 2.56 g (77%) of the title product, m.p. 150-3 °C. (b) N-ben__yl-2.2.2-trifluoro-N-( ' 4-nitrobenzvDacetamide This compound benzyl-(4-nitrobenzyl)amine was prepared using the method described in Example 10, step (b) for the preparation of benzyl-3- (nitrobenzyl)amine. From 2.49 g (9.36 mmol) of N-(4-nitrobenzyl)-benzamide and 18.6 ml of 1.0 M borane in THF was obtained 3.12 g of the crude benzyl-(4- nitrobenzyl)amine, which was used without further purification. This crude product was mixed with 4.3 ml of triethylamine in 40 ml of methylene chloride at 0oC under nitrogen. To this solution was added dropwise 3.6 ml of trifluoroacetic anhydride. The solution was stirred for 10 minutes and was poured into water and separated. The dried organic phase was dried (MgSO 4 ) and concentrated to give 3.1 g (94%) of the title compound as an oil. (c) N-.4-aminobenzylVN-benzyl-2.2.2-trifluoroacetamide This compound was prepared using the method described in Example 10, step (c) for the preparation of N-(3-aminobenzyl)-N-benzyl-2,2,2-trifluoroacetamide. From N-benzyl-2,2,2-trifluoro-N-(4-nitroben__yl)acetamide (3.1 g, 9.2 mmol) was obtained 2.46 g (78%) of the title compound as the hydrochloride salt following hydrogenation. Recrystallization from isopropanol and ether gave 1.74 g of pure material, mp 115-9 °C. (d) N-('4-f rphenylmethyl)amino ' .methvπphenylV2-thiophenecarboximidamide From 0.60 g (1.9 mmol) of the free base of N-(4-aminobenzyl)-N-benzyl-2,2,2- trifluoroacetamide and 0.42 g (1.6 mmol) of S-methyl 2-thiophenethiocarboximide (prepared by taking the hydrochloride salt produced by following a method analogous to that of Example 1, step (d), and neutralizing with 2.5 m NaOH and extracting into methylene chloride) in 4 ml of isopropanol. The reaction mixture was stirred for 5 h. A solution (2 ml) of 2.5 M sodium hydroxide and about 5 drops of methanol was added and the resulting solution was heated at reflux for 1 h. The solution was concentrated and the product was extracted into ethyl acetate. The solution was dried and concentrated to give a sohd. This was converted to the bis oxalate in isopropanol and then recrystallized from 95% ethanol to give 110 mg (10%) of the title compound, p 209-13 °C. Example 12 The following compounds were prepared following the method of Example 1: (a) N-.4-, 2-f r2.6-dichlorophenyl )methyl )ammo )ethvπphenylV 2-thiophenecarboximidamide m.p. 104-105 °C (b) N-f4-r2- rr2-bromophenyl lmethyl )amino .ethyl )phenylV2- thiophenecarboximidamide m.p. 81-82 °C (c) N-r3-('2- Phenylmethvπamino')ethvnphenylV3-thiophenecarboximidamide dihvdrochloride. m.p. 145-147 °C (d) N-f4-f2-f.2.6-dichlorophenylmethvDam.no thvnphenyl , -3-thiophenecarboximidamide free base. m.p. 109-110 °C (e) N-, 4-f2-aminoethvDphenylV3-thiophenecarboximidamide dihvdrobromide m.p. 158-170 dec (f) N- 4-f2-(T2.6-dichlorophenylmethyl)amino')ethyl lphenylV2-furanocarboximidamide free base, m.p. 101-104 °C (g) N-f3-f3- i-pyrolidinyl > lpropyπphenylV2-thiophenecarboximidamide free base. m.p. 110-111 °C (h) N- 4-C2-aminoethyl )phenylV2-furocarboximidamide dioxalate. m.p. 162 °C dec Example 13 The following compounds were prepared following the method of Example 9: (a) N-, 4-ffl-piperidinvDmethvπphenylV2-thiophenecarboximidamide dihvdrobromide m.p. 277-278 °C (b) N-r4- i-pyττolidinvπmethvπphenylV2-thiophenecarboximidamide dihvdrobromide m.p. 248-250 °C Example 14 The following compound was prepared following the method of Example 10: N-.3-Crfphenylmethvπamino)methyl ' .phenylV2-thiophenecarboximidamide dimaleate m.p. 171-173 °C Example 15 The following compound was prepared following the general method of Example 1 starting at step (e) by reacting S-methyl-2-thiophenecarboximide hydroiodide with 3-(methylamino)phenylamine. N-f3-, famino1methyl .phenylV2-thiophenecarboximidamide dimaleate m.p. 145-148 °C Example 16 The following compound was prepared following the method of Example 10 N-f3-f2-ffphenylmethvDamino thvDphenviy2-thiophenecarboxamidine Dioxalate. m.p. 132-134 °C Example 17 N-f3-f2-rEthylamino N )ethvπphenylV2-thiophenecarboximidamide (a) f3-Nitrophenvnacetyl chloride A stirred solution of 3-nitrophenylacetic acid (10.0 g, 55.2 mmol) in thionyl chloride (100 ml, 1.37 mol) was heated at reflux for 2 hours, then concentrated to yield 11.1 g of (3-nitrophenyl)acetyl chloride as a tan solid. (b) N-Ethyl-2-C3-nitrophenyl)acetamide To a stirred solution of 70 wt % ethylamine in water (35 ml) cooled on an ice bath was added in one portion (3-nitrophenyl)acetyl chloride (3 g, 15.0 mmol). Resulting mixture was warmed to achieve a clear solution, allowed to cool. Resulting precipitate was filtered to yield N-ethyl-2-(3-nitrophenyl)acetamide as a yellow solid: (2.2 g, 71%); m.p. 115-117 °C. (c) EthvH2-f3-nitrophenvnet_.vDam.ne hydrochloride To a stirred solution of the product of step (b) (2.2 g, 10.6mmol) in tetrahydrofuran (50ml) under nitrogen, was added dropwise 1.0M borane-tetrahydrofuran (42ml, 42mmol). Reaction was heated at reflux for 1.5 hours, cooled on ice bath, then aqueous 6N HCl (75ml) added dropwise. The resulting mixture was refluxed for 1 hour, basified to pH 11 with 20% aqueous sodium hydroxide, extracted twice with ether. Combined extracts were dried over magnesium sulfate, filtered, concentrated. The hydrochloride salt of the crude was made from isopropanol and ethyl acetate to yield ethyl-(2-(3-nitrophenyl)ethyl)amine hydrochloride as a light yellow solid: (1.7g, 70%); m.p. 186-188 °C. (d) 3-.2-Ethylamino-ethyl ,phenylamine hydrochloride To a solution of the product of step (c) (1.7g, 7.0mmol) in methanol (30ml) was added a catalytic amount of 10% palladium on carbon. The mixture was hydrogenated at 50 psi for 30 minutes, filtered through celite, concentrated to yield 3-(2-ethylamino-ethyl)phenylamine hydrochloride as an off-white solid: (1.4g, 100%); m.p. 192-194 °C. (e) N-f3-f2-fEthylamino)ethvDphenylV2-thiophenecarboximidamide dihvdrobromide To a solution of the product of step (d) (1.4g, 7.0mmol) in isopropanol (20ml) and dimethylformamide (20ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (2.5g, 8.8mmol). The mixture was stirred for 16 hours, diluted with 20% aqueous sodium hydroxide, and extracted twice with ethyl acetate. The combined extracts were washed twice with water, dried over magnesium sulfate, filtered, and concentrated to give 2.7g of an oil. The dihydrobromide salt was made from isopropanol and ethyl acetate, recrystallized from isopropanol, methanol, and ethyl acetate to yield N-(3-(2-(ethylamino)ethyl)-phenyl)-2- thiophenecarboximidamide dihydrobromide as a tan solid: (1.72g, 49%); m.p. 192- 194 °C (dec). Example 18 N-f3-f3-ffPhenylethvnamino .propynphenviy2-thiophenecarboximidamide dioxalate (a) 3-f3-Phenylethylamino-propyQphenylarnine dihvdrochloride This was prepared following a method analogous to that of Example 17, steps (a)- (d). (b) N-f3-f3-ffPhenylethvπamiπo propynphenvπ-2-thiophenecarboximidamide dioxalate To a solution of the product of step (a) (3.0 g, 9.17 mmol) and S-methyl-2- thiophenethiocarboximide hydroiodide (3.3 g, 11.5 mmol) in isopropanol (25ml) and dimethylformamide (25ml) was added pyridine (0.74 ml, 9.17 mmol) in one portion. The mixture was stirred for 16 hours, diluted with 20% aqueous sodium hydroxide, and extracted twice with ethyl acetate. The combined extracts were washed twice with water, dried over magnesium sulfate, filtered, and concentrated. The dioxalate salt of the crude was made from ethanol and ether, recrystallized from ethanol to yield N-(3-(3-((phenylethyl)amino)propyl)phenyl)-2-thiophenecarboximidamide dioxalate as the white solid: (2.3 g, 44%); m.p. 102-105 °C. Example 19 N-f3-f2-fff2-Bromophenyl .methyl .amino .ethyl .pheπvn-2-thiophenecarboximidamide (a) N-f2-Bromobeπ_-ylV2-f3-nitrophenyl .acetamide This was prepared following a method analogous to that of Example 17, steps (a)- (b). (b) N-f2-Brornobenzyl .-2-f3-arninophenvOacetan.ide To a solution of the product of step (a) (5.45 g, 15.6 mmol) in 85% glacial acetic acid (400 ml) was added in one portion the zinc dust (10.2 g, 156 mmol). Reaction was stirred for 30 minutes, filtered, and concentrated. The residue was partitioned with 20% aqueous sodium hydroxide and dichloromethane, the organic layer was dried over magnesium sulfate, filtered, concentrated to yield N-(2-bromobenzyl)-2- (3-aminophenyl)acetamide as a white solid: (4.7 g, 94%); m.p. 110-112 °C. (c) 3-f2-f2-Bromobeπzylamino .ethyl .pheπylamine dihvdrochloride This was prepared following a method analogous to that of Example 17, step (c). fd . N-f3-f2-fff2-Bromophenvnmethvnamino .ethyl ,phenvn-2- thiophenecarboximidamide dioxalate This was prepared following a method analogous to that of Example 18, step (b). m.p. 175-8 °C (dec) Example 20 N-f3-f2-fPhenylamino .ethyl .phenylV2-thiophenecarboximidan.ide dihvdrobromide (a) f2-f3-NitrophenvQethyl ,phenylamine This was prepared following a process analogous to that of Example 17, steps (a)- (c). (b) 2.2.2-Trifluoro-N-f2-f3-nitrophenyl > )ethyl>N-phenylacetamide This was prepared following a process analogous to that of Example 1, step (a). fc . 2.2.2-Trifluoro-N-f2-f3-aminophenvπethylVN-phenylacetamide This was prepared following a process analogous to that of Example 1, step (c). (d) N-f3-f2-fPhenylamino .ethv1.phenv1V2-thiophenecarboximidarnide dihvdrobromide This was prepared following a process analogous to that of Example 1, step (e). m.p. 235-240 °C (dec). Example 21 The following compounds were prepared following a process analogous to that of Example 17: (a) N-f4-f2-fEthylamino ethv0phenv1V2-thiophenecarboximidan.ide dihvdrobromide. m.p. 176-178 °C (b) N-f4-f2-f2-Propylamino^ethv0phenylV2-thiophenecarboximidamide dihvdrobromide. m.p. 240-242 °C (dec) (c) N-f4-f2-fl-Propylamino)ethvπpheπyl ,-2-thiophenecarboximidamide dihvdrobromide. m.p. 233-235 °C (dec) (d) N-f4-f2-ft-Butylamino1ethvnphenylV2-thiophenecarboximidamide dihvdrobromide. m.p. 241-242 °C (e) N-f4-f2-fn-Butylamiπo thv1 ,phenylV2-thiophenecarboximidarnide dihvdrobromide. m.p. 238-240 °C (f) N-f3-f2-fMethylamino .ethvnphenylV2-thiophenecarboximida ide dihvdrobromide. m.p. 219-223 °C (g) N-f3-f2-f l-Propylamino .ethyl .pheπv1.-2-thiophenecarboximidarnide dihvdrobromide. m.p. 72-75 °C (softened) (h) N-f3-f2-ft-Butylamino .ethv0phenyl ,-2-thiophenecarboxirnidamide dihvdrobromide. m.p. 232-235 °C (dec) (i) N-f3-f2-f2-Propylamino)ethyl .phenvπ-2-thiophenecarboximidamide dihvdrobromide, m.p. 206-210 °C (dec) (j) N-f3-f2-aminoethyl .phenylV2-thiophenecarboximidamide dihvdrobromide. m.p. 194-199 °C (k) N-f3-f2-fDimethylamino .ethvOphenv1V2-thiophenecarboximidamide dihvdrobromide, m.p. 232-233 °C (dec) (1) N-f3-f2-fDiethylamino .ethyl .phenyl .-2-thiophenecarboximidarnide dihvdrobromide. m.p. 75-80 °C (softened) (m) N-f3-f2-f2-f l.2.3.4-Tetrahvdro )isoquinolinyl .ethvnphenylV2- thiophenecarboximidamide dioxalate. m.p. 172-175 °C (dec) (n) N-f4-f3-f2-fl.2.3A-tetrahydro ,isoquinolyl ,propyl .phenyl ,-2-thiophene carboximida ide dioxalate. m.p. 138-142°C (o) N-f4-f2-f3.5-bistrifluoromethylphenylmethvπamino)ethyl .phenyl-2- thiophenecarboximidamide free base, m.p. 98-100 °C (p) N-f4-f2-fdiethylamino .ethvI .phenvI-2-thiophenecarboxirnidarnide free "base, m.p. 113-115 °C (q) N-f4-f2-ff3-chlorophenylmethvnamino .ethvOphenyl ,- benzenecarboximidamide dihvdrochloride, 253-254°C (r) N-f4-f2-ff3-chlorophenylmethyl .amino thvOphenyl .-3-chlorothiophene-2- carboximidamide dihvdrochloride. 257°C (s) N-f4-f2-ff4-methylphenylmethyl )amino)ethyl )phenylV2-thiophenecarboxamidine dihvdrochloride. 218-219°C (t) N-f4-f2-fpiperonylamino .ethyl .phenv1 )-2-thiophenecarboxamidine dihvdrochloride. m.p. 205-6 °C Example 22 The following compounds were prepared following a process analogous to that of Example 18: (a) N-f3-f2-fff2-Chlorophenyl .methyl .amino .ethyl .phenyl .-2- thiophenecarboximidamide dioxalate. m.p. 155-157 °C (dec) (b) N-f3-f3-ffPhenylmethyl .amino .propyπphenvπ-2-thiophenecarboximidamide dioxalate, m.p. 138-141 °C (dec) (c) N-f4-f2-fff3-Chloronhenyl .methvnamino .ethyl .phenyl .-2- thiophenecarboxamidine dioxalate. m.p. 216-217 °C (d) N-f4-f2-fff4-Chloroohenyl .methyl .amino .ethyl .phenyl .-2- thiophenecarboxamidine dioxalate. m.p. 203-204 °C (e) N-f4-f2-fff3-Chlorophenvnmethyl .amino .ethvnphenvn-3-chlorothiophene- 2-carboximidamide dihvdrochloride m.p. 257-258°C (f) N-f4-f3-fethylamiπo .propyl .pheπyl-2-thiophenecarboximidaιτιide dioxalate. m.p. 98-100 °C Example 23 The following compound was prepared following a process analogous to that of Example 19: (a) N-f3-f2-ffN-Pheπylmethyl-N-methvnamiπo thyl .phenvn-2- thiophenecarboximidamide. free-base m.p. 85-87 °C (b) N-f4-f2-ffN-Phenylmethyl-N-methvnamino .ethvnphenylV2-thiophene carboximidamide. free base m.p. 110-112 °C (c) N-f3-f2-fff3-Chlorophenyl .methyl .amino )ethvnpheπylV2- thiophenecarboximidamide dioxalate. m.p. 185-88 °C (dec) (d) N-f3-f2-fff3-fluorophenv1.methyl .amino1ethvnphenv1.-2- thiophenecarboximidamide dioxalate. m.p. 183-4 °C (e) N-f4-f3-fff3-Chlorophenyl .methv1.amino .propynphenyl>2-thiophene carboximidamide dioxalate. m.p. 212-215 °C (f) N-f4-f3-ffphenylmethyl-N-methynamino)propynphenvn-2-thiophene carboximidamide dihvdrobromide. m.p. 228-232°C(dec) (g) N-f4-f2-ffethvnfphenv1methyl .amino ' ethvnphenvn-2- thiophenecarboximidamide free base. m.p. 87-89 °C (h) N-f4-f2-ffpropyl .fphenylmethvnamino .ethvnphenvn-2- thiophenecarboximidamide free base. m.p. 100-102 °C (i) N-f4-f2-ffl.l-dimethylethyl .fphenylmethvnamino ethyl .phenvn-2- thiophenecarboximidamide free base, m.p. 145-148 °C (j) N-f4-f2-fff3.4-dichlorophenyl . methyl . amino)ethyl .phenvn-2- thiophenecarboximidamide free base, m.p. 111-114 °C Example 24 N-f4-f3-ffphenylamino .carboπynpropynphenvn-2-thiophenecarboximidamide (a) 4-f3-ffphenylarnino )carbonvnpropyl .ani1ι ' ne A stirred solution of 4-(4-nitrophenyl)butyric acid (5.0 g; 0.023 moles) in 20 ml of thionyl chloride was refluxed for 4 hours. The solvent was evaporated and the crude acid chloride. 2.5g, was added dropwise to a stirred solution of aniline (2.0 g; 0.02 moles) in 30 ml of tetrahydrofuran and 10 ml of triethylamine and the reaction was then stirred 18 hours. The triethylamine hydrochloride was removed by filtration and 50 ml of ethyl acetate was added to the organic phase. The organic phase was washed with 1 x 100 ml of 1 N hydrochloric acid and the dried over magnesium sulfate. The solvent evaporated to yield a yellow solid. The solid was dissolved in 100 ml of methanol and 250 mg of 10% palladium on carbon was added, the reaction was hydrogenated over a 4 hour period. The product was found to be unreduced, and was then dissolved in 100 ml of methanol. 10 ml of a saturated solution of HCl in isopropanol was added, followed by 250 mg of 10% palladium on carbon. The mixture was hydrogenated for 4 hours. The catalyst was removed by filtration and the solvent evaporated. The residue was dissolved in 100 ml of hot water with a minimal amount of methanol, and the solution was then made basic with 50% sodium hydroxide solution. The mix was extracted with 150 ml of ethyl acetate, the extract was dried with magnesium sulphate and evaporated, giving solid 4-(3-(((phenyl)amino)carbonyl)propyl)aniline, yield 1.0 g, one spot on TLC. (b) N-f4-f3-ffphenv1amino)carbonvnpropynphenvn-2- thiophenecarboximidamide To a stirred suspension of the product of step (a) (1.00 g; 0.0037 moles) in approximately 5ml of isopropanol was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (l.Olg, 0.0035 moles). This mixture was refluxed for 1 hr and then allowed to cool giving solids. The product was filtered and allowed to dry under vacuum overnight giving N-(4-(3- ((phenylamino)carbonyl)propyl)phenyl)-2-thiophenecarboximidamide. 1.76 g yield. m.p. 229-231 °C. Example 25 The following compounds was prepared following a process analogous to that of Example 24: (a) N-f4-f3-ffphenylmethylamino ,carbonyl .propyl .phenyl .-2- thiophenecarboximidamide. m.p. 169-171 °C. (b) N-f4-f3-ff l-pyrrolidyl .carbonyl .propyloxy .phenyl .-2- thiophenecarboximidamide. m.p. 191-194 °C (c) N-f4-f3-ff4-morpholinyl .carbonyl ,propyloxy .phenyl .-2- thiophenecarboximidamide. m.p. 136-138°C (d) N-f4-f2-ffphenylmethylamino )carbonvnethvnphenvn-2- thiophenecarboximidamide. m.p. 63-65°C (e) N-f3-f2-ffphenv1amino .carbonyl .ethvnphenvn-2-thiophenecarboximidamide, m.p. 203-205°C (f) N-f4-f3-ffphenv1amino .carbonvnpropynphenvn-2-pyrrolecarboximidamide, m.p. 195-196°C (g) N-f4-f3-ffphenv1amino )carbonvnpropyl .phenvn-2- furocarboximidamide, m.p. 197-199°C (h) N-f4-f3-ffphenylamino1carbonyl .propyl .phenyl .-3-chloro-2- thiophenecarboximidamide. m.p. 141-144°C (i) N-ff3-ffphenv1amiπo .carbonv1.propynphenvn-l-methylpyrrole-2- carboximidamide, m.p. 154-155°C (j) N-f4-f3-f - 4-methv1piperazinyl arbonv1.propyl .phenvD-2- thiophenecarboximidamide. m.p. 132-134 °C Example 26 N-f4-f3-ff l-pyrro1idvncarbonv1.propyl .phenyl . thiophene-2-carboximidamide hydroiodide (a) 4-f3-ff l-pyrrolidinyl .carbonyl .propyl .aniline 4-(4-nitrophenyl)butyric acid (2.25 g, 0.01076 moles) was dissolved in 40 ml dichloromethane and cooled to -5 °C on an ice/acetone bath. Triethylamine (1.09 g, 0.01076 moles) and ethyl chloroformate (1.17 g, 0.01076 moles) were added and the mixture was stirred for 10 minutes before pyrrolidine (0.92 g, 0.01291 moles) was added dropwise whilst maintaining the temperature below 0 °C. After 10 minutes the cold bath was removed and the reaction stirred for 16 hours at room temperature. The dichloromethane solution was washed with 2 x 75 ml saturated sodium bicarbonate and 2 x 75 ml water. The dichloromethane layer was dried over magnesium sulfate and the solvent evaporated under vacuum to leave 2.19 g of a clear brown oil. The clear brown oil was reduced under 50 psi hydrogen; ethanol was the solvent and 10% palladium on carbon was used as a catalyst. After 4 hours the catalyst was filtered off and the solvent evaporated under vacuum to provide 4- (3-((l-pyrrolidinyl)carbonyl)propyl)aniline, an oil that solidified on standing (this was used as is). (b) N-f4-f3-ffl-pyrrolidv1.carbonynpropynphenvn-2-thiophenecarboximidamide hydroiodide S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (1.53 g 0.00538 moles) was added to the product of step (a) (1.50 g 0.00646 moles), in 6 ml of isopropanol and stirred at room temperature for 16 hours. The resulting suspension of solids was diluted with 50 ml isopropanol and the solids collected by filtration to give N-(4-(3-((l-pyrrolidyl)carbonyl)propyl)phenyl)-2- thiophenecarboximidamide hydroiodide, mp 213-216 °C. Example 27 The following compounds were prepared following a process analogous to of Example 26: (a) N-f4-f3-ff4-morpholinvncarbonyl .propyl .phenyl .-2- thiophenecarboximidamide hydroiodide. m.p. 189-192 °C. (b) N-f4-ffphenylamino)carbonvnphenvn-2- thiophenecarboximidamide, m.p. 200-201°C (c) N-f4-f2-ff4-morpholinyl .carbonyl .ethyl .phenvn-2-methylthiazole-4- carboximidamide. m.p. 280-281 °C Example 28 N-f4-f2-fff4-morpolinvncarbonyl .amino > )ethvnphenvnthiophene-2-carboximidamide hydroiodide (a) 4-f2-fff4-morpolinvncarbonvnamino )ethyl . aniline hydrochloride To a stirred solution of 4-nitrophenethylamine hydrochloride (4.0 g; 0.24 moles) in 50 ml of tetrahydrofuran was added 10 ml of triethylamine. To this was added 4- morpholinecarbonyl chloride (3.6 g; 0.024 moles) dropwise in 20 ml of tetrahydrofuran and the reaction stirred for 6 hours. The triethylamine salt was removed by filtration, and the organic phase was washed with 1 x 100 ml of 1 N hydrochloric acid. The organic phase was dried over magnesium sulfate; evaporation of the solvent gave a crude oil. The crude oil was then dissolved in 250 ml of methanol, to this was added 250 mg of 10% palladium on carbon and the reaction hydrogenated for 4 hours. The catalyst was removed by filtration, and the solvent evaporated. To the residue was added 150 ml of ethyl acetate and hydrochloric acid gas was then added to make the salt. Upon cooling 4-(2-(((4- morpolinyl)carbonyl)amino)ethyl)aniline hydrochloride, a pink solid, crystallized and was collected by filtration, 4.4 g. (b) 4-f2-fff4-morpolinyl .carbonyl .amino .ethvnaniline The product of step (a), 4.4 g, was dissolved in 200 ml of water. The mixture was made basic with 50 % sodium hydroxide solution, and extracted with 2 X 50 ml of ethyl acetate. The ethyl acetate extracts were combined, dried with magnesium sulfate, and evaporated in vacuo, giving a solid. The solid was dissolved in hot ethyl acetate and hexane, and crystals of 4-(2-(((4-morpolinyl)carbonyl)amino) ethyl)aniline formed on cooling. Analysis: calculated C 62.63 H 7.68 N 16.85; found C 62.43 H 7.65 N 16.59. (c) N-f 4-f2-fff4-morpolinyl .carbonvnamino > )ethyl .phenyl ,-2- thiophenecarboximidamide hydroiodide The product of step (b), 1.0 g, and S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) 1.09 g, were combined in a minimal amount of isopropanol. The mixture was refluxed for one hour. The resulting solution was cooled, solids precipitated and were collected by fitration, giving N-( 4-(2-(((4-morpolinyl)carbonyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide hydroiodide, 0.95 g, m.p. 209-211 °C Example 29 hydroiodide (a) 4-f4-nitrophenoxy .butrvic acid Ethyl 4-bromobutyrate, 9.9 g, 4-nitrophenol, 7.0 g, and sodium carbonate, 6.0 g, were combined in 50 ml of DMF, and the mixture was warmed on a 100 C hot plate for four hours. The solids were removed by filtration, and washed with 20 ml of acetone, the filtrate was diluted to 400 ml with cold water, and extracted with a combination of 50 ml ethyl acetate and 50 ml of hexanes. The resulting organic layer was washed with 3 X 200 ml of 0.2 M potassium carbonate to remove unreacted nitrophenol. The resulting organic layer was evaporated in vacuo to give 11 g of yellow oil. The crude yellow oil was diluted with 200 ml of methanol, treated with 25 ml of 2 M sodium hydroxide, and stirred over night at room temperature. The mixture was evaporated in vacuo, and diluted with water to 250 ml. The solution was clairified with celite, and then acidified with 20 ml of 4 M hydrochloric acid. The resulting solids were collected by filtration, washed with water, and dried in vacuo to give 4-(4-nitrophenoxy)butryic acid, mp 116-118 °C. (b) 4-f3-fffphenyl .amino .carbonyl .propy1oxy . aniline A solution of 4-(4-nitrophenoxy)butryic acid, 4.0 g, in 20 ml of thionyl chloride was refluxed for four hours, and then the excess thionyl chloride was evaporated in vacuo. The crude acid chloride was the added to a solution of aniline, 1.68 g, and triethylamine, 10 ml, in 30 ml of THF, and the reaction was stirred for 18 hours. The solids were removed by filtration, and the filtrate was diluted with 50 ml of ethyl acetate. The solution was washed with 100 ml of 1 N hydrochloric acid, dried with magnesium sulfate, and evaporated to give a solid. The solid was disolved in 100 ml of methanol, and hydrogenated with with 10 % Pd/C for a total of 40 hours, giving 1.25 g of 4-(3-(((phenyl)amino)carbonyl)propyloxy)aniline, a white solid, M.S. (M+H) + = 271. (c) N-f4-f3-fffpheπvnamino carbonv1.propyloxy .phenvn-2- thiophenecarboximidamide hydroiodide The product of step (b), 1.0 g, and S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)), 1.01 g, were combined in a minimal amount of isopropanol and the mixture refluxed for one hour. The resulting clear solution was cooled, solids precipitated and were collected by filtration, giving N-(4-(3-(((phenyl)amino)carbonyl)propyloxy)phenyl)-2- thiophenecarboximidamide hydroiodide, 1.76 g, m.p. 229-231 °C. Example 30 N-f4-f2-ffftrifluoromethv1.carbonyl .amino ethvnphenyl .-2- thiophenecarboximidamide (a) 4-f2-ffftrifIuoromethyl .carbonyl .amino .ethvnnitrobenzene To a stirred solution of 4-nitrophenethylamine hydrochloride (1.84 g, 9.10 mmol) and triethylamine (3.03 ml, 21.70 mmol) in methanol (12 ml) was added trifluoroacetic anhydride (1.51 ml, 10.66 mmol) dropwise. After stirring for 1 minute, the solvent was removed at reduced pressure and the remaining residue was mixed with water and extracted with methylene chloride (3 X 20 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to yield a solid which was recrystallized from methylene chloride/hexane to give 4-(2-(((trifluoromethyl)carbonyl)amino)ethyl)nitrobenzene as a white solid: 1.92 g (80% yield); m.p. 103-104 °C. (b) 4-f2-fff trifluoromethyl . carbonvnamino .ethvnaniline To a stirred solution of the product of step (a) (0.52 g, 1.98 mmol) in THF MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give 4- (2-(((trifluoromethyl)carbonyl)amino)-ethyl)aniline which was homogeneous by TLC and used immediately in the next reaction. (c) N-f4-f2-ffftrifluoromethvncarbonyl .amino .ethvnphenyl .-2- thiophenecarboximidamide To a solution of the product of step (b) (0.30 g, 1.29 mmol) in isopropanol (6 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (0.37 g, 1.29 mmol). The mixture was stirred for 4 hr, turned into a solution of saturated NaCl (50 ml) and 50% NaOH (4 ml), and extracted with ethyl acetate (3 X 20 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered and concentrated to a solid which was recrystallized from hexane/ethyl acetate to yield N-(4-(2-(((trifluoromethyl) carbonyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide as a slightly yellow solid: 0.19 g (43% yield), m.p. 181-182 °C. Example 31 N-f4-f2-fffmethvncarbonvnamino )ethyl .phenvn-2-thiophenecarboximidamide (a) 4-f2-fffmethvncarbonyl .amino .ethyl .nitrobeπzene The above compound was made by the mothod of Example 30 step (a) except that trifluoroacetic anhydride was replaced by acetic anhydride. 1.28 g of a pale yellow solid was obtained that was used immediately in the next reaction. (b) 4-f2-fffmethv1.carbonyl .amino')ethv1. niline To a stirred solution of the product of step (a) (0.82 g, 3.94 mmol) in THF/MeOH (100 ml, 1:1) was added 4 ml of 1 N HCl and a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 4 hr, filtered through celite, and concentrated to give a solid. The solid was turned into a solution of saturated Nad (50 ml) and 50% NaOH (4 ml), and extracted with ethyl acetate (3 X 20 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered and concentrated to a solid which was used immediately in the next reaction, (c) N-f4-f2-fffmethvncarbonyl .amino .ethvnphenvn-2-thiophenecarboximidamide The above compound was prepared following the procedure of Example 30, step (c). After recrystallization from ethyl acetate/methanol, 0.56 g of a tan solid was obtained, mp. 186-187 °C. Example 32 N-f4-f2-fffphenylmethyl .carbonyl .amino .ethv1.phenvn-2-thiophenecarboximidamide (a) 4-f2-fffphenylmethyl .carbonvnamino .ethyl . nitrobenzene The above compound was prepared following the procedure of Example 30, step (a) except that trifluoroacetic anhydride was replaced by phenylacetyl chloride. 1.42 g of a pale yellow solid was obtained that was used immediately in the next reaction. (b) 4-f2-fffphenylmethyl arboπvnamiπo .ethyl . aniline The above compound was prepared by an analogous process to that described in Example 8. step (b). The oil obtained was used immediately in the next reaction. (c) N-f4-f2-fffphenylmethvncarbonvnamino .ethv1.phenv1.-2- thiophenecarboximidamide The above compound was prepared following the procedure of Example 30, step (c). After recrystallization from ethyl acetate/methanol, 0.45 g of a tan solid was obtained, mp. 210-211 °C. Example 33 N-f4-f2-fffphenvncarbonyl .amino ' ethvnphenvn-2-thiophenecarboximidamide (a) 4-f2-fffphenvncarbonyl .amino .ethyl . nitrobenzene The above compound was prepared following the procedure of Example 30, step (a) except that trifluoroacetic anhydride was replaced by benzoyl chloride. 1.77 g of a pale yellow solid was obtained that was used immediately in the next reaction. (b) 4-f2-fffphenyl ,carbonyl .amino . ethyl . aniline The above compound was prepared by an analogous process to that described in Example 8, step (b). The oil obtained was used immediately in the next reaction. (c) N-f4-f2-fffphenvncarbonvnamino .ethv1.phenvn-2-thiophenecarboximidamide The above compound was prepared following the procedure of Example 30, step (c). After recrystallization from ethyl acetate/methanol, 1.10 g of a tan solid was obtained, m.p. 196-197 °C. Example 34 N-f4-fffphenvniminocarbonyl .amino .phenylV2-thiophenecarboximidamide fa) N-f4-nitrophenvnbenzenecarboxirnidarnide To a stirred solution of benzonitrile (25 ml) was added a catalytic amount of 4- dimethylaminopyridine. To this was added 4-nitroaniline hydrobromide (10:0 g, 0.57 moles) and the reaction then heated to 190 °C for six hours. The reaction mixture was allowed to cool and 25 ml isopropanol was added. A solid was collected by filtration to yield 8.6 g of N-(4-nitrophenyl)benzenecarboximidamide, m.p.. 240-241 °C (b) N-, 4-aminophenv] .benzenecarboxirπidamide To a pressure bottle charged with the product of step (a) (8.6 g, 0.024 moles) in 200 ml of methanol was added 20 ml of isopropanol/HCl and 0.5 g of 10% palladium on carbon. The reaction was hydrogenated for six hours; the catalyst was removed by filtration and the solvent evaporated. To the residue was added 50 ml of isopropanol and 100 ml of ethyl acetate, the solid was slurried and then collected by filtration to yield 10.6 g of N-(4-aminophenyl)benzenecarboximidamide hydrochloride. The N-(4-aminophenyl)benzenecarboximidamide hydrochloride was dissolved in 100 ml of water and 20 ml of 50% sodium hydroxide. The aqueous phase was extracted (3x100 ml) of ethyl acetate, and dried over magnesium sulfate. Evaporation of the solvent gave solid product N-(4-aminophenyl)benzene carboximidamide, yield 7.6 g. fc . N-f4-fffphenvniminocarbonvnamiπo)phenvn-2-thiopheπecarboximidamide To a stirred suspension of the product of step (b) (l.lg, 0.0052 moles) in 5 ml of isopropanol was added S-methyl-2-thiophenecarboxthioimide hydroiodide (the product of Example 1, step (d)) (1.5 g, 0.0054 moles) and the reaction was stirred for 48 hours. The solid was collected by filtration, this was dissolved in a solution containing 100 ml of water and 10 ml of 50% sodium hydroxide and the aqueous phase was extracted three times with ethyl acetate (100ml). The organic phase was dried over magnesium sulfate and subsequent evaporation of the solvent gave a crude oil. The crude oil was dissolved in 20 ml of methanol and HCl gas was added; upon standing a solid crystallized and was collected by filtration. The product, N-(4-(((phenyl)iminocarbonyl)amino)phenyl)-2-thiophenecarboximidamide, was dried at 80 °C for 24hrs. m.p. 317-318 °C Example 35 The following compounds were prepared following a method analogous to that of Example 34: (a) N-N"-f l.4-phenv1ene .bis-2-thiophenecarboximidamide hydroiodide. m.p. 278-279 °C (b) N-N"-fl.3-pheπv1ene .bis-2-thiophenecarboximidamide. m.p. 219-220 °C (c) N.N'-f l.3-phenylene .bis-2-chlorophenylcarboximidamide dimaleate, m.p. 200-201 °C (d) N.N'-f 1.4-phenylene .bis-3-chlorothiophene-2-carboximidamide free base. m.p. 247-248 °C (e) N-f4-fff2-methoxyphenyl .imiπocarboπyl .amino .pheπvn-2-thiophene carboximidamide free base, m.p. 187-188 °C (f) N-f4-fffPhenvniminocarbonyl .amino .phenyl .-3-chlorothiophene -2-carboximidamide free base, m.p. 213-214 °C (g) N-f4-fffPhenyl ,iminocarbonyl .amino .phenyl .-3-thiophene carboximidamide dihvdrochloride. m.p. 323-324 °C (h) N-f3-fffpheπvniminocarbonyl,amino,pheπylV2-thiophenecarboximidamide m.p.295-296 °C (i) N-f4-fff4-chlorophenyl.iminocarbonyl.amino.phenyl.-2- thiophenecarboximidamine dihvdrochloride, m.p.296-297 °C (j) N-f4-fff2-chlorophenyl.iminocarbonyl.amino.phenyl.-2- thiophenecarboximidamide dioxalate. m.p.166-167 °C (k) N-f4-fff4-bromophenyl.iminocarbonvnamino.phenyl.-2- thiophenecarboximidamide dioxalate. m.p.236-237 °C (1) N-f4-fff3-chloro-4-methylphenyl.iminocarbonv1.amino)pheπyl.-2- thiophenecarboximidamide dihvdrochloride, m.p.294-294 °C (m) N-f4-fff3.5-dimethoxyphenyl.iminocarbonyl.arnino.phenyl.-2- thiophenecarboximidamide dioxalate. m.p.226-227 °C (n) N-f4-fff3.5-dichlorophenyl,iminocarbonyl,arnino.phenyl.-2- thiophenecarboximidamide dioxalate. m.p.237-238 °C (o) N-f4-fffPhenyl.iminocarbonyl.amino,phenyl.-2-furancarboximidamide dioxalate m.p. 210-211 °C (p) N-f4-fff3-methylphenyl.iminocarbonyl.amino.phenyl.-2- thiophenecarboximidamide free base m.p.205-206 °C (q) N-f4-fff3-Methoxyphenyl.iminocarbonyl.amino.phenvn-2- thiophenecarboximidamide free base m.p.194-195 °C (r) N-f4-fff3-Bromophenyl.iminocarbonyl.amiπo.phenvn-2- thiophenecarboximidamide dihvdrobromide m.p.293-294 °C (s) N-f4-fff3-chlorophenyl,iminocarbonyl.amino.pheπyl.-2- thiophenecarboximidamide dihvdrochloride m.p.310-311 °C (t) N-f4-fff3-methylphenyl.iminocarbonyl.amino)phenyn-2-pyrrolecarboximidamide dihvdrobromide m.p. 210-211 °C (u) N-f4-fff4-chlorophenyl.iminocarbonvnamiπo.phenvn-2-pyrrolecarboximidamide difumarate m.p.228-229°C Example 36 N-f4-f2-fffPhenylamiπo .carbonvnamino .ethyl .phenylV2-thiophenecarboxamidine hydroiodide (a) N-f2-f4-Nitrophenvnethyl .N'phenyl urea Prior to running the reaction, a 3.09g sample of 4-nitrophenylamine hydrochloride was dissolved in 20 mis water and treated with 30 mis 2N NaOH. The freebase was extracted with 2x75 mis diethyl ether. The ether layer was dried over magnesium sulfate, and the volume of the ether was reduced under vacuum to —60 mis. To this solution was added 1.81g of phenyl isocyanate dropwise. The white solids that precipitated upon addition were stirred for three hours, then were collected by filtration and washed with ether. Air drying left 3.67g N-(2-(4- Nitrophenyl)ethyl)N'phenyl urea, m.p. 170-172 °C. (b) N-f2-f4-aminophenvnethvnN , phenyl urea To a pressure bottle charged with 3.67g N-(2-(4-Nitrophenyl)ethyl)N'phenyl urea in 100 mis of a 50/50 volume mix of methanol/THF was added a catalytic amount of 5% Pd/C. The mix was hydrogenated under 50 psi hydrogen gas for 24 hours, the catalyst was filtered and TLC showed starting material and two lower Rf spots. The solvents were removed by evaporation in a vacuum and the resultant solid was taken up in methanol, and an excess of oxalic acid was added. The solution was quenched with ether and the precipitated white solids were collected by filtration. The solids were treated with 100 mis 2N NaOH, and the freebase was extracted with ethyl acetate; the organic layer was dried with magnesium sulfate and ' evaporated to leave a yellow solid; N-(2-(4-aminophenyl)ethyl) N'phenyl urea (430 mg). (c) N-f4-f2-fffPhenylamino .carbonyl .amino .ethyl .phenyl .-2- thiophenecarboxamidine hydroiodide. To a solution of 430mg N-(2-(4-Nitrophenyl)ethyl)N'phenyl urea slurried in 3 mis of isopropyl alcohol was added 435 mg of S-methyl-2-thiophenecarboxamide dihydroiodide (the product of Example 1, step (d)). The mix was stirred for 16 hrs at room temperature. The suspension was diluted in 25 mis isopropyl alcohol and the solids were collected by filtration to leave a yellow/tan solid. The solids were recrystallized from methanol/ether. Two batches were collected and combined to give 470 mg of N-(4-(2-(((Phenylamino)carbonyl)amino)ethyl)phenyl)-2- thiophenecarboxamidine hydroiodide, m.p. 216-219°C. Example 37 The following compound was made by a process analogous to that of Example 37: N-f4-f2-fffphenylamino .carbonyl .oxy .ethvnphenvn-2-thiophenecarboximidamide, m.p. 222-224°C Example 38 N-f4-ffbisfphenylmethyl .amino .methvnphenvn-2-thiophenecarboximidamide (a) 4-ffbisfphenylmethyl .amino ,rnethyl .nitrobenzene To 4-nitrobenylamine (1.61 g, 10.60 mmol) in DMF (25 ml) was added potassium carbonate (3.22 g, 23.30 mmol) followed by benzyl bromide (2.64 ml, 22.30 mmol). The mixture was allowed to stir for 2 days, dumped into water and extracted with ethyl acetate (3 X 50 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated and chromatographed over silica gel (20% ethyl acetate hexane) to yield 4-((bis(phenylmethyl)amino)methyl) nitrobenzene: (1.64 g, 47%); M.S. (M+H) + = 333. (b . 4-ffbisfphenylmethvnamino .methyl . aniline To the product of step (a) (0.56 g, 1.69 mmol) in AcOH (15 ml) was added Tin(II) chloride dihydrate (2.00 g, 19.03 mmol) followed by concentrated HCl (5 ml). The mixture was stirred for 20 hr, cooled to 0 °C, quenched with 50% NaOH, and extracted with ethyl acetate (3 X 50 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated and chromatographed over silica gel (30% ethyl acetate/hexane) to yield 4-((bis(phenylmethyl) am_no)methyl)aniline: (0.29 g, 57%); M.S. (M+H) + = 303. (c) N-f4-ffbi.sfphenylmethyl .amino)methvnphenvn-2-thiophenecarboximidamide To a solution of the product of step (b) (0.28 g, 0.93 mmol) in isopropanol (6 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (0.26 g, 0.93 mmol). The mixture was stirred for 14 hr, quenched with 2 N NaOH (2 ml) and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to a solid which was recrystallized from ethyl acetate/hexane to yield N-(4-((bis(phenylmethyl)amino)methyl)phenyl) -2-thiophenecarboximidamide as a white solid: (86 mg, 22%); m.p. 127-128 °C. Example 39 The following compound was prepared following a process analogous to that of Example 8: N-f4-f2-aminomethyl .phenyl .-2-thiophenecarboximidamide hvdrobromide. m.p.188- 189 °C. Example 40 N-f3-fl.2.3.4-tetrahvdroisoquinolin-2-ylmethvnphenvn-2-thiophenecarboximidamide dihvdrobromide (a) 3-f l,2.3.4-tetrahvdroisoquinolin-2-ylmethyl . nitrobenzene To 3-nitrobenzyl chloride (2.00 g, 11.66 mmol) in DMF (25 ml) was added potassium carbonate (1.93 g, 13.96 mmol) followed by tetrahydroisoquinoline (1.55 g, 11.66 mmol). The mixture was allowed to stir for 4 hr, dumped into water and extracted with ethyl acetate (3 X 50 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to an oil. The oil was dissolved in ether and treated with IPA/ΗC1 to afford 3-(l,2,3,4-tetrahydroisoquinolin-2-ylmethyl)nitrobenzene hydrochloride: (1.96 g, 55%); m.p. 196-197 °C. (b) 3-f l.2.3.4-tetrahvdroisoquinolin-2-ylmethyl .aniline hydrochloride To a stirred solution of the product of step (a) (l.OOg, 3.29 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 0.5 hr, filtered through celite, and concentrated to give 3-(l,2,3,4-tetrahydroisoquinolin-2-ylmethyl)aniline hydrochloride which was homogeneous by TLC and used immediately in the next reaction. (c) N-f3-fl.2.3.4-tetrahvdroisoquinolin-2-ylmethyl .phenylV2- thiophenecarboximidamide To a solution of the product of step (b) (0.90 g, 3.29 mmol) in isopropanol (3 ml)/DMF (1 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (0.94 g, 3.29 mmol). The mixture was stirred for 14 hr, quenched with 2 N NaOH (2 ml) and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and chromatographed over silica gel (8% methanol/methylene chloride) to yield the title compound as the free base. Treatment with IPA/HBr yielded N-(3-(l,2,3,4-tetrahydroisoquinolin-2-ylmethyl)phenyl)-2-thiophenecarboximidamide as a white solid: (0.26 g, 16%); m.p. dec > 179 °C. Example 41 N-f4-f4-ffphenylmethyl .amino ,butyl .phenyl .-2-thiophenecarboximidamide dimaleate (a) 4-f4-nitrophenvn-N-fphenylrnethvnbutylamide A sample of 4-(4-nitrophenyl)butyric acid, 2.09 g, dissolved in 20 ml of dichloromethane was treated with benzylamine, 1.07 g, giving suspended solids. The mix was treated with diphenylphosphorylazide, 2.75 g, and 20 ml of dioxane and on stirring for 4 hours it became clear. The mix was diluted with 100 ml of ethyl acetate and washed twice with 100 ml of 2 M potassium carbonate, then 100 ml of 1 M hydrochloric acid. The organic layer was dried with magnesium sulfate and evaporated to give solids. The solids were dissolved in 150 ml of cyclohexane and 50 ml of ethyl acetate, and cooled to give white solids; these solids were collected by filtration and air dried to give 4-(4-nitrophenyl)-N- (phenylmethyl)butylamide mp 133-135 °C (b) 4-f4-aminophenyl ,-N-fphenylmethyl .hutylamide hydrochloride A sample of the product of step (a), 0.80 g in 20 ml of ethanol and 20 ml of ethyl acetate was treated with 0.4 g of 5 % paladium on carbon and placed under 50 psi of hydrogen. TLC after one hour showed a new spot, Rf 0.2 with 15% acetone in methylenechloride. The mix was evaporated to dryness, and then treated with 30 ml of toluene. The residue was dissolved in 10 ml of THF and treated with 5 ml of 1 M lithium aluminium hydride in THF (Aldrich) giving a clear solution. After one hour at room temperature, 1 ml of 2M sodium hydroxide was added dropwise, followed by 10 g of anhydrous sodium sulfate. After stirring for 30 minutes, the mix was filtered and treated with hydrogen chloride gas and an oil formed. The mix was treated with 3 ml of isopropanol, and further hydrogen chloride gas, giving solids. The mix was cooled to -20 °C for 2 hours, then filtered and air dried to give 4-(4-aminophenyl)-N-(phenylmethyl)butylamide hydrochloride; Chloride analysis: calc 20.78 found 20.64 (c) 4-f4-ffphenylmethynamino^butyl .aniline dihvdrochloride A sample of the product of step (b) was suspended in 5 ml of THF and treated with 5 ml of 1 M lithium aluminium hydride in THF (Aldrich) giving a clear solution. The mixture was warmed to reflux for 5 hours, then cooled. The semisolid mass was diluted with diethyl ether to 20 ml, and then 1 ml of 2M sodium hydroxide was added dropwise, followed by 4 cm 3 of anhydrous sodium sulfate. After stirring for 15 minutes, the mix was filtered and the solids were washed with 20 ml of diethyl ether. The combined filtrates were treated with hydrogen chloride gas and allowed to stand at room temperature. The solids that formed were collected by filtration and dried in vacuo to give 4-(4- (phenylmethyl)amino)butyl)aniline dihydrochloride; Chloride analysis: calc 21.66 found 21.63 (d) N-f4-f4-ffphenylmethvnamino )butvnphenvn-2-thiophenecarboximidamide dimaleate A sample of the product of step (c), 0.50 g, and S-methyl-2- thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)), 0.44g were combined in 4 ml of isopropanol and warmed to 60 °C. After 2 hours, TLC with 15 % methanol in chloroform on silica showed that the starting aniline was mostly consumed, and that there had developed a new spot of lower Rf. The mix was diluted with 20 ml of 1M potassium carbonate, and extracted with ethyl acetate. The ethyl acetate extract was dried with 10 g of potassium carbonate and treated with 0.150 g of maleic acid, giving a gummy precipitate. TLC of the precipitate versus the supernatant showed the precipitate to be a mix of the starting amine and product, and that the supernatant contained mostly product. The supernatant was then treated again with 0.150 g of maleic acid, giving a gummy precipitate, and the remaining supernatant was decanted. The solids were dissolved in 5 ml of methanol and precipitated with 100 ml of diethylether. The resulting gummy precipitate was reacted with 1 ml of water, diluted with acetone to 200 ml giving a clear solution, diluted with diethyl ether to 275 ml and cooled to -20 °C. The solids were collected by filtration, washed with 20 ml of diethyl ether, and dried in vacuo to provide N-(4-(4-((phenylmethyl)amino)butyl)phenyl)-2- thiophenecarboximidamide dimaleate, m.p. 104-106 °C Example 42 N-f4-ffff2-thiopheπvniminomethvnamino .methyl .phenvn-2- thiophenecarboximidamide dit ' umarate (a) 4-aminomethylaniline To a solution of 4-nitrobenzylamine hydrochloride (9.0g, 0.0477) in methanol (200ml) was added 20ml of IPA/HCl and a catalytic amount of 10% palladium on carbon. The mixture was hydrogenated at 50 psi for 4hrs, filtered through celite, concentrated to a solid. The above solid was then dissolved in 300ml of water and 20ml of 2N sodium hydroxide and extracted into methylene chloride (3x100ml). The combined extracts were dried over magnesium sulfate, filtered and concentrated to give 4-aminomethylaniline as an oil (6.1g). (b) N-f4-ffff2-thiophenyl ,iminomethyl .amino .methyl .phenyl .-2- thiophenecarboximidamide difumarate To a stirred solution of 4-aminomethylaniline (1.6g, 0.0013mmol) in (10ml) dimethylformamide and (10ml) of isopropanol was added S-methyl-2- thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (4.4g, 0.015mmol). The mixture the heated to 40 °C for 72 hrs. The reaction was diluted with 20% aqueous sodium hydroxide, and the solids collected by filtration to yield (2.5g). The fumaric acid salt was made from isopropanol and methanol, to yield (2.0g) of N-(4-(((2-thiophenyl)iminomethyl)amino)methyl)phenyl)-2- thiophenecarboximidamide difumarate, m.p. 200-201 °C Example 43 The following compounds were prepared by a process analogous to that of Example 17: (a) N-f3-f3-fl-pyrrolidinvnpropynphenyl .-phenylcarboximidamide dioxalate, m.p. 138-139 °C (b) N-f4-f2-ff4-methoxyphenylmethyl .amino .ethyl .phenyl . -2-thiophenecarboximidamide free base, m.p. 144-145 °C (c) N-f4-f2-ff4-methylphenylmethvnamino lethvnphenvn-2- thiophenecarboximidamide monohvdrochloride. m.p. 225-226 °C (d) N-f3-f2-ff3-phenylpropynamino .ethvnphenvn-2-thiophenecarboximidamide dihvdrobromide m.p. 183-186 °C (e) N-f3-f2-ff2-methylpheπylmethvnarnino .ethyl .phenyl .-2- thiophenecarboximidamide free base m.p. 114-116 °C (f) N-[4-f2-ff l-indanyl .ethyl .amino')phenyl]-2-thiophenecarboximidamide dioxalate, m.p. 95 °C (dec) (g) N-f4-f2-fff4-pyridyl .methyl .amino s )ethyl .phenvn-2-thiophenecarboximidamide trihvdrochloride m.p. >250°C (h) N-f4-f2-fff2-thienyl .methvnamino)ethvnphenvn-2-thiophenecarboximidamide dioxalate m.p. 226-227°C Example 44 N-f3-f2-ff2-phenylethyl .amino .ethvnphenyl .-2-thiophenecarboximidamide dihvdrobromide (a) N-f2-phenylethvn-3-nitrophenylacetamide 2.50g (0.0138 moles) of 3-nitrophenylacetic acid were refluxed in 25mls of thionyl chloride. The solvents were evaporated and the residue taken up in 50mls of THF before 3.51 g (0.290 moles) of phenethylamine were added dropwise at 0 °C. The mix was stirred for 48 hours and the hydrochloride salt of the amine was filtered off as a white solid. The wash was evaporated to leave N-(2-phenylethyl)-3- nitrophenylacetamide, a dull orange oil, 4.48 g. The product was analyzed by MS and NMR. (b) N-f2-phenylethviy2-f3-nitrophenyl .ethylamine To a stirred solution of the product of step (a), 4.48g, (0.0158moles) in 80ml dry THF was added 47.4 ml of 1M Borane/THF. The mix was heated to reflux for 3 hours and cooled before 10 ml of methanol was added carefully followed by 20 ml of 4N HCl. The solution was concentrated by evaporation under vacuum to leave a reddish liquid. The oil was basified with 2M NaOH and the product extracted with 3x50ml of EtOAc. The organic layers were combined, dried over magnesium sulfate and evaporated to leave an oil. The oil was dissolved in HCl/ isopropanol solution. White solids formed and were collected by filtration to give N-(2- phenylethyl)-2-(3-nitrophenyl)ethylamine, 2.63g, m.p. 196-200 °C. (c) N-f2-phenylethvn-N-f2-f3-nitrophenvnethvntrifluoroacetamide To a slurry of the product of step (b), 2.63g, (0.00857moles) in 40ml dichloromethane was added 1.99g (0.0197 moles) of triethylamine and the mix was cooled to 0 °C before 2.34g (0.111 moles) of trifluoroacetic anhydride was added dropwise. After 45 minutes, the mixture was quenched with 50ml water and the product extracted with 3x50ml dichloromethane. The organic layers were combined, dried over magnesium sulfate and evaporated to leave N-(2-phenylethyl)- N-(2-(3-nitrophenyl)ethyl)trifluoroacetamide, 3.3g, as an oil. (d) N-f2-phenylethyl .-N-f2-f3-amiπophenyl .ethyl .trifluoroacetamide To a solution of the product of step (c), 3.3g, in 75ml each of THF and methanol was added a catalytic amount of 10% Pd on carbon. After 1 hour under 50 psi hydrogen, the reaction was complete. The catalyst was filtered off and the solvents evaporated to leave N-(2-phenylethyl)-N-(2-(3-aminophenyl)ethyl) trifluoroacetamide, 2.88g, as an oil. (e) N-f3-f2-ff2-phenylethyl .amino1ethyl .phenv1.-2-thiophenecarboximidamide dihvdrobromide To a solution of the product of step (d), 2.88g (0.00857 moles) in 15 ml isopropanol was added 2.94g of S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)). The mixture was stirred at room temperature for 16 hours then a solid yellow residue was filtered off and discarded. The wash was evaporated and the residue dissolved in minimal methanol; the solution was basified with 2M NaOH and heated to 50 °C for 30 minutes. The deprotected product was extracted with 3x50ml ethyl acetate, the organic layers were combined, washed with 2x50ml water, dried over magnesium sulfate and evaporated to leave an oil. The free base was dissolved in an isopropanol solution of HBr. Solids formed with the addition of ethyl acetate and cooling were collected by filtration to give 102 mg of N-(3-(2-((2-phenylethyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide dihydrobromide salt. m.p. 137-139 °C. Example 45 The following compounds were prepared by following a method analogous to that of Example 44: (a) N-f4-f2-aminoethyl ,phenylV2-pyιτolecarboximidamide dioxalate m.p. 145 °C (dec) (b) fSVN-f4-f2-ffl-phenylethvnamino^.ethvnphenvn-2-thiophenecarboximidamide dihvdrochloride m.p. 197 °C dec (c) fRVN-f4-f2-ffl-phenylethvnamino^.ethvnphenvn-2-thiophenecarboximidamide free base, m.p. 92-94 °C (d) N-f3-f2-ff4-phenylbutvnamino )ethvnphenyl ' .-2-thiophenecarboximidamide dihvdrobromide. m.p. 136-139 °C Example 46 N-f4-fffphenylmethoxy ' .carbonvnaminomethvnphenvn-2-thiophenecarboximidamide oxalate (a) 4-fffphenylmethoxy^carbonynaminomethyl ' mitrobenzene A sample of 4-nitrobenzylamine hydrochloride, 5 g, and 200 ml of water was treated with sodium bicarbonate, 10 g. The mixture was treated with 50 ml of ethyl acetate, and then benzyl chloroformate, 4 ml. After 4 hours, the mix was treated with 200 ml of hexanes, and the precipitated solids were collected by filtration. The crude product was dissolved in 150 ml of hot methanol, filtered, diluted with 250 ml of water, and cooled. The resulting solids were collected to give 4-(((phenylmethoxy)carbonyl)aminomethyl)nitrobenzene, m.p. 92-93 °C. (b) 4-fffphenylmethoxy.carbonynaminomethvnaniline The product of step (a), 6.0 g was treated with 10 ml of acetic acid and 100 ml of methanol. The mixture was treated with platinum sulfide on carbon, 0.97 g, and treated with 50 psi of hydrogen gas; after 20 hours, the mixture was filtered and evaporated in vacuo. The residue was dissolved in 50 ml of ether, and diluted with hexanes to 200 ml. The mixture was then stirred for five days and filtered to give 4-(((phenylmethoxy)carbonyl)aminomethyl)ani_ine, m.p. 60-64 °C (c) N-f4-fffphenylmethoxy^carbonvnammomethyl ,phenyl ,-2- thiophenecarboximidamide oxalate The product of step (b), 0.89 g, and S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)), 1.0 g, were combined in 6 ml of isopropanol, and stirred at 30 °C. After 4 hours, the mixture was precipitated with ether, giving gummy solids which were taken up in 100 ml of hot water, treated with cellite and filtered. The mix was cooled, giving gummy white solids which were treated with 50 ml of ethyl acetate and 5 g sodium bicarbonate. The ethyl acetate layer was dried with magnesium sulfate, and cooled to -20 °C. The mix was then treated with hexanes, and failed to give crystals. The mix was evaporated in vacuo, and the crude free base, 0.7 g, was dissolved in 40 ml of warm isopropanol and treated with oxalic acid dihydrate, 0.26 g. On cooling and treatment with 150 ml of ether a tacky precipitate resulted. After stirring overnight at room temperature, the resulting solids were collected by filtration to yield N-(4-(((phenylmethoxy)carbonyl)aminomethyl)phenyl)-2-thiophene carboximidamide oxalate, m.p. 150-160 °C Example 47 N-f4-f2-f(phenylmethvnamino ethoxy1phenyn-2-thiophenecarboximidamide hydroiodide (a) 4-nitrophenoxy-N-fphenylmethyl .acetamide A sample of 4-nitrophenoxy-N-(phenylmethyl)acetic acid hydrazide (Lancaster), 4.22 g, was treated with 20 ml of 1 M aqueous HCl and 200 ml of ethyl acetate. The mix was cooled to 10 °C, and then 1.38 g of sodium nitrite in 20 ml of water was added over 2 minutes. The mix was stirred for 5 minutes, the layers were separated, and the ethyl acetate layer was dried with sodium sulfate. The ethyl acetate solution was treated with 5 ml of benzylamine, giving a prompt precipitate formation. After 20 minutes the mix was washed with 100 ml of saturated sodium carbonate, then 100 ml of 1 M HCl (aqueous). The ethyl acetate layer was then evaporated. The solids were dissolved in 50 ml of acetone, and precipitated with water. The solids were collected by filtration to provide 4-nitrophenoxy-N-(phenylmethyl)acetamide, m.p. 125-126 °C, 3.76 g. (b) 4-aminόphenoxy-N-fphenylmethyl .acetamide A sample of the product of step (a), 3.74 g, was taken up in 100 ml of methanol and 100 ml of ethyl acetate. The mix was treated with 0.4 g of 10 % palladium on carbon and placed under 50 psi hydrogen. After one hour the mix was filtered, and concentrated in vacuo to provide crude 4-aminophenoxy-N-(phenylmethyl) acetamide: CHN calculated: C 70.29, H 6.29, N 10.93, found C 69.97, H 6.3, N 10.90 (c) 4-f2-ffphenylmethyl .amino .ethoxy . aniline A solution of 3.2 g of the product of step (b) in 40 ml of dry THF under N 2 was treated with 40 ml of 1 M diborane in THF. The mix was warmed to reflux for three hours, treated with 40 ml of 6M aqueous HCl, and refluxed for 2 hours. The filtrate was concentrated under vacuo to 150 ml. The cloudy mix was treated with 100ml of crushed ice and neutralised with 50 % NaOH, and the resulting fine solids were collected, washed with water, and dried by infrared to give 4-(2- ((phenylmethyl)amino)ethoxy)aniline, MS = 243, 98 % by capillary electrophoresis (d) N-f4-f2-ffphenylmethyl .amino .ethoxy phenvn-2-thiophenecarboximidamide hydroiodide A sample of 4-(2-((phenylmethyl)amino)ethoxy)aniline, 0.81 g-and S-methyl-2- thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)), 1.42 g were combined in 10 ml of isopropanol and stirred for 7 hours at room temperature. The resulting white solids were collected by filtration, washed with 10 ml of isopropanol, and dried in vacuo to provide white solids, N-(4-(2- ((phenylmethyl)amino)ethoxy)phenyl)-2-thiophenecarboximidamide hydroiodide, m.p. 193-195 °C. Example 48 N-[4-fffDiphenylamino .carbonvnamino .phenyl]-2-thiophenecarboxamidine hydrochloride (a) 4-[Diphenylaminofcarbonyl ,amino]aniline To a stirred solution of 1,4-phenylenediamine (1.00 g, 9.25 mmol) and triethylamine (1.29 ml, 9.25 mmol) in methylene chloride (50 ml) was added diphenylcarbamyl chloride (2.14 g, 9.25 mmol). After stirring for 14 hr, the mixture was dumped into water and extracted with methylene chloride (3 X 20 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, concentrated and chromatographed over silica gel (80% ethyl acetate/hexane) to yield 4-[diphenylamino(carbonyl)amino]aniline: (0.49 g, 17%); M.S. (M+H) + = 304. (b) N-[4-fffDiphenylamino .carbonyl .amino .phenyl]-2-thiophenecarboxarnidine hydrochloride To a solution of the product of step (a) (0.49 g, 1.62 mmol) in isopropanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (0.46 g, 1.62 mmol). The mixture was stirred for 48 hr, dumped into basic water and extracted with ethyl acetate ( 3 X 30 ml). The combined extracts were washed with water, dried over magnesium sulfate, filtered, and concentrated to an oil. Treatment with IPA/HC1 yielded N-[4- (diphenylamino(carbonyl)amino]phenyl-2-thiophenecarboxamidine hydrochloride as a white solid: (24 m g, 3.3%); m.p. 210-211 °C. Example 49 N-f3-ffbenzovnamino)phenv1V2-thiophenecarboximidamide oxalate (a) N-f3-nitrophenvnbenzamide To a solution of 7.5 g (54 mmol) of 3-nitroaniline in a biphasic solution consisting of 100 ml of methylene chloride and 100 ml of 20% potassium carbonate was added dropwise 6.0 ml (52 mmol) of benzoyl chloride in 25 ml of methylene chloride. The reaction mixture was allowed to stir overnight and the organic phase was separated and washed with dilute hydrochloric acid. The solvent was concentrated to give 3.83 g (32%) of the title compound, MS 243 (M+H). (b) N-f3-amiπophenvnbenzamide This compound was prepared following a process analogous to that of Example 30 step (b). MS 213 (M+H). (c) N-f3-ffbenzoyl .amino phenyl>2-thiophenecarboximidarnide oxalate This compound was prepared following the procedure of Example 30, step (b). The free base was converted to the oxalate salt in isopropanol. MS 323 (M+H). Example 50 N-f4-ffbenzoyl .amino .phenylV2-thiophenecarboximidarnide hydroiodide (a) N-f3-nitrophenyl .benzamide This compound was prepared following a process analogous to that of Example 49, step (a). MS 243 (M+H). (b) N-f4-aminophenyl .benzamide This compound was prepared following a process analogous to that of Example 30, step (b). MS 213 (M+H). (c) N-f4-ffbenzovnamino)phenyl .-2-thiophenecarboximidarnide hvdroiodide This compound was prepared following the procedure of Example 26, step (b) and was recrystallized from water, m.p. 234-5 °C. Example 51 N-f3-fffphenylamino')carbonvnamino .phenyn-2-thiophenecarboximidamide oxalate (a) N-phenyl-N'-f3-nitrophenyl , urea To a solution of 5.0 g (36 mmol) of m-nitroaniline in 40 ml of ether was added 5.0 ml (47 mmol) of phenylisocyanate. The solution was stirred for 6 hours. The product was filtered to afford 9.2 g (99%) of the title compound, MS 258 (M+H). (b) N-phenyl-N'-f3-aminophenyl . urea This compound was prepared by a process analogous to that of Example 30, step (b). Slurring the isolated product in ether afforded a solid product, m.p. 199-202 °C. (c) N-f3-fffphenylamino .carbonv0amino ,phenylV2-thiophenecarboximidamide oxalate This compound was prepared following the procedure of Example 30, step (c). The free base was converted to the oxalate salt in isopropanol, 208-210 °C. Example 52 N-f3-fff4-phenoxy1butyl .amino .carbonyl .phenylV2-thiophenecarboximidamide oxalate (a) 3-nitro-N-f4-phenoxybutyl .benzamide This compound was prepared following a process analogous to that of Example 49, step (a). MS 315 (M+H). (b) N-ff3-f4-phenoxylbutyl .amino ,carbonyl , aniline hydrochloride A solution of 7.8 g (25 mmol) N-4-phenoxybutyl-3-nitrobenzamide and 1 g of 5% palladium on carbon in 120 ml of isopropanol with hydrogen chloride added was hydrogenated at 45 psi for 3 hr. The catalyst was filtered off and the solvent was concentrated to give 6.7 g (84%) of the title compound, MS 285 (M+H). (c) N-f3-fff4-phenoxylbutyl .amino .carbonvnphenvn-2-thiophenecarboximidamide oxalate The above compound was first converted to the free base and the title compound was prepared using the procedure of Example 30, step (c). The free base of the title compound was then converted to the oxalate salt in isopropanol. MS 394 (M+H), m.p. 154-6 °C. Example 53 N-f3-fff4-phenylbutvnamino )carbonyl .phenvn-2-thiophenecarboximidamide oxalate (a) 3-nitro-N-f4-phenylbutyl .benzamide This compound was prepared following a process analogous to that of Example 49, step (a). MS 299 (M+H). (b) 3-amino-N-f4-phenylbutyl .benzamide hydrochloride This compound was prepared following a process analogous to that of Example 52, step (b). MS 273 (M+H). (c) N-f3-fff4-phenylbutyl .amino .carbonv1.phenvn-2-thiophenecarboximidamide oxalate This compound was prepared following the procedure of Example 30, step (c) except an equivalent of triethylamine was also added. The free base was converted to the oxalate salt in isopropanol, MS 376 (M+H), m.p. 118-120 °C. Example 54 N-f4-ffffbenzvnamino)carbonyl .methvnphenvn-2-thiophenecarboximidamide (a) N-beπzyl-f4-nitro .phenylacetamide This compound was prepared following a process analogous to that of Example 49, step (a), m.p. 172-82 °C. (b) N-benzyl-f4-amino .phenylacetamide This compound was prepared following the procedure of Example 17, step (d), m.p. 137-140 °C. (c) N-f4-ffffbenzyl .amino ' carbonvnmethvnphenvn-2-thiophenecarboximidamide This compound was prepared following the procedure of Example 30, step (c), m.p. 157-161 °C. Example 55 N-f4-f2-fl-pyrrolidiπyl .ethyl .phenylV2-thiophenecarboximidamide dihvdrobromide (a) N-pyrrolidiπyl-f4-nitrophenyl .acetic acid To a solution of 3.12 g (43.9 mmol) of pyrrolidine in a biphasic solution consisting of 100 ml of methylene chloride and 100 ml of 20% potassium carbonate was added dropwise 7.3 g (36.5 mmol) of 4-nitrophenylacetyl chloride in 25 ml of methylene chloride. The reaction mixture was allowed to stir overnight and the organic phase was separated and washed with dilute hydrochloric acid. The solvent was concentrated to give 6.26 g (73%) of the title compound, m.p. 103-5 °C. (b) 4-f2-fl-pyrrolidinyl .ethvnnitrobenzene This compound was prepared following the procedure of Example 17, step (c). MS 221 (M+H). (c) 4-f2-fl-pyrrolidinv1.ethvnaniline This compound was prepared following the procedure of Example 34, step (c). MS 191 (M+H). (d) N-f4-f2-f l-pyrrolidinyl .ethyl .phenvn-2-thiophenecarboximidamide dihvdrobromide This compound was prepared following the procedure of Example 18, step (b). The dihydrobromide salt was crystallised from isopropanol and ether, MS 300 (M+H). Example 56 N-f4-f2-f l-piperidinyl .ethyl .phenvn-2-thiophenecarboximidamide dihvdrochloride (a) N-piperidinyl-f4-nitrophenyl .acetic acid This compound was prepared following the procedure of Example 56, step (a), m.p. 105-7 °C. (b) N-f4-f2-fl-piperidinvnethvnnitrobenzene This compound was prepared following the procedure of Example 17, step (c), MS 235 (M+H). (c) N-f4-f2-f l-piperidinyl .ethvnaniline This compound was prepared following the procedure of Example 34, step (c). The hydrochloride salt was converted to the free base as an oil, MS 205 (M+H). (d) N-f4-f2-fl-piperidinvnethvnphenylV2-thiophenecarboximidamide dihvdrochloride This compound was prepared following the procedure of Example 34, step (d). The dihydrochloride salt was crystallised from isopropanol and ether, m.p. 256-61 °C. Example 57 N-f4-f3-fl-pyrrolidinyl .propynphenyl ,-2-thiophenecarboximidamide dioxalate (a) N-pyrrolidinyl-f4-nitrophenyl .propenamide This compound was prepared following the procedure of Example 55, step (a), MS 247 (M+H). (b) 4-f2- l-f fpyrolidinvncarbonyl .ethyl . aniline This compound was prepared following the procedure of Example 34, step (c). MS 219 (M+H). (c) 4-f3-fpyrolidinyl .propyl . aniline dihvdrochloride This compound was prepared following the procedure of Example 17, step (c). The dihydrochloride salt from ethanol, m.p. 262-5 °C. (d) N-f4-f3-fl-pyrrolidinyl .propy1 ,phenv1V2-thiophenecarboximidamide dioxalate This compound was prepared following the procedure of Example 18, step (b). The dioxalate salt was prepared from ethanol and ether, m.p. 86-92 °C. Example 58 N-f4-f3-fl-piperidinyl .propynphenvn-2-thiophenecarboximidamide dioxalate (a) N-piperidinyl-f4-nitropheπyl .propenamide This compound was prepared following the procedure of Example 55, step (a), m.p. 168-71 °C. (b) 4-f2-f -ffpiperidinyl .carbonvnethyl .aniline This compound was prepared following the procedure of Example 34, step (c). MS 233 (M+H). (c) N-f4-f2-f l-piperidinyl .propyl .aniline This compound was prepared following the procedure of Example 17, step (c), m.p. 180-5 °C. (d) N-f4-f3-f l-piperidinyl .propyl .phenyl .-2-thiophenecarboxirnidamide dioxalate This compound was prepared following the procedure of Example 17, step (e). The dioxalate salt was prepared from ethanol and ethyl acetate, MS 328 (M+H). Example 59 N-f4-f2-f2-fl.2.3.4-tetrahvdro)isoquinolyl .ethyl .phenvn-2-thiophenecarboximidamide dihvdrobromide (a) 4-nitro-N-f2-isoquinolyl .phenylacetamide To a solution of 4-nitrophenylacetic acid (5.43 g 930 mmol) and 1,2,3,4- tetrahydroisoquinoline (5.6 g, 42 mmol) in methylene chloride (200 ml) was added l-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (6.13 g, 32 mmol). The reaction mixture was allowed to stir for 18 hr. The reaction mixture was washed with dilute hydrochloric acid and dilute sodium hydroxide, dried, and the solvent was concentrated to give a sohd. Trituration with ether gave the title compound, m.p. 137-9 °C. (b) N-f4-f2-f2-fl.2.3.4-tetrahvdro .isoquinolvnethvnnitrobenzene This compound was prepared following the procedure of Example 17, step (c), m.p. 97-102 °C. (c) N-f4-f2-f2-fl.2.3.4-tetrahvdro soquinolvnethvnaniline This compound was prepared following the procedure of Example 34, step (c), m.p. 300 °C (dec). (d) N-f4-f2-f2-fl.2.3.4-tetrahvdro .isoquinolyl .ethvnphenvn-2- thiophenecarboximidamide dihvdrobromide This compound was prepared following the procedure of Example 18, step (b). The dihydrobromide salt was prepared from ethanol and ethyl acetate, MS 362 (M+H). Example 60 N-f4-ffffphenylmethvnamino)methylcarbonvnamino^.phenvn-2- thiophenecarboximidamide free base (a) N-f4-nitrophenyl ,-2-chloroacetamide 4-nitroaniline (13.8 g) in ethyl acetate (200 ml) was treated with triethyl amine (15 ml) and then treated in portions with chloroacetyl chloride (8 ml). The resulting mixture was stirred for 10 minutes. The mixture was then treated with water (200 ml) and ethyl acetate (100 ml). The mixture was warmed until all solids were dissolved, and the layers separated. The ethyl acetate layer was then concentrated to 100 ml whilst hot, and cooled to room temperature. The next day the mixture was filtered, and the solids washed with ethyl acetate and air dried to give N-(4- nitrophenyl)-2-chloroacetamide, m.p. 183-185 °C, 14.83 g. (b) 4-ffffphenylmethvnamino')methylcarbonynamino')nitrobenzene The compound of step (a) (4.28 g) and benzylamine (2.5 ml) were combined in DMF (10 ml) with potassium carbonate (3.2 g) and stirred at room temperature for 3 hours. The solids were filtered off, and washed with methanol (2 X 10 ml). The combined filtrates were slowly diluted with water to 150 ml to give yellow solids, which were collected by filtration, and air dried to give 4- ((((phenylmethyl)amino)methylcarbonyl)amino)nitrobenzene, MS 286 (M+H). (c) 4-ffffphenylmethvnftrifluoromethylcarbonvnamino^methylcarbonyl, aminolnitrobenzene The compound of step (b) (4.9 g) trifluoroacetic anhydride (2.5 ml) and triethylamine (2.5 ml) were combined in ethyl acetate (50 ml), and the mixture with suspended solids was warmed to 50 °C overnight. The mixture was then washed with water (50 ml), filtered to remove solids, and the ethyl acetate layer was evaporated. The residue was taken up in ether (150 ml) and cooled to -20 °C overnight. The solids were collected by filtration to give 4- ((((phenylmethyl)(trifluoromethylcarbonyl)amino)methylcarbonyl)-amino)- nitrobenzene, MS (M+H) = 282. (d .4-ffffphenylmethvnftrifluoromethylcarbonvnamino )methylcarbonyl .amino )aniline The compound of step (c) (3.8 g) was dissolved in ethyl acetate (50 ml) and ethanol (50 ml). The mixture was hydrogenated at 50 psi with palladium on carbon for 4 hours. The mixture was filtered and evaporated in vacuo to give white solids, 4- ((((phenylmethyl)(trifluoromethylcarbonyl)amino)methylcarbonyl)amino)-aniline, MS (M+H)=352. (e) N-f4-ffffphenylmethvnamino1methylcarbonynamino^phenvn-2- thiophenecarboximidamide free base The compound of step (d) (1.05 g) and S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1 step (d)) (0.85 g) were treated with methanol (2 ml). After 15 minutes the solids had dissolved and the mixture was blown down with nitrogen to remove methanethiol. TLC with 10 % isopropanol in chloroform on silica showed starting amine consumed and new spot of lower Rf. The mixture was dissolved in methanol (6 ml), and treated with potassium carbonate (1.1 g). The TLC with 15 % methanol in chloroform on silica showed incomplete hydrolysis, so an additional 1.1 g of potassium carbonate was added. After 2 hours the conversion was complete, and the mix was filtered to remove solids. The next day the filtrate was treated with 0.65 g of maleic acid, diluted with ether and stirred overnight. The solids that were collected were not the desired product. The filtrate was further diluted with hexane, and washed with water. The aqueous layer was treated with 1 M potassium carbonate, and extracted with ethyl acetate. The ethyl acetate was evaporated in vacuo, and the residue taken up in 20 ml of methanol. The solution was treated slowly with water until solids precipitated, the solids were collected by filtration and dried at 40 °C under vacuum to give N-(4- ((((phenylmethyl)amino)methylcarbonyl)amino)phenyl)-2- thiophenecarboximidamide, free base, m.p. 161-163 °C. Example 61 N-f4-f2-fff2-Furanyl ,methyl .amino )ethyl .phenvn-2-thiophenecarboximidamide dioxalate (a) 4-Nitrophenyl acetyl chloride To 4-nitrophenyl acetic acid (30 g) was added thionyl chloride (100 ml). The mix was heated to reflux under nitrogen and stirred for two hours. The excess thionyl chloride was evaporated under vacuum, and the remaining oil was azeotropically dried with toluene. The resulting oil crystallized upon standing to leave 4- nitrophenyl acetyl chloride (35 g). (b) 4-f2-fff2-Furanynmethvnamino )ethvnnitrobenzene hydrochloride To a stirred solution of furfurylamine (1.32 g) in methylene chloride (125 ml) at °C was added triethyl amine (2.36 ml), followed by the dropwise addition of a solution of the compound of step (a) (3.0 g) in methylene chloride (10 ml). The mixture was stirred at 0 °C for 15 minutes. The mixture was poured into water (150 ml) and the crude products extracted with methylene chloride (2 X 100 ml). The organic layers were collected, dried (MgSO 4 ), filtered and concentrated. The resulting sohds were taken up in a solution of 7% methanol/methylene chloride, and purified on a silica gel column eluted with the same solvent. The product was collected and concentrated. The solid was taken up in THF (50 ml) and treated with 1M Borane/THF (50 ml). The solution was refluxed for 15 hours. The mixture was cooled to 0 °C and was slowly made acidic with 4N hydrochloric acid. The mixture was reheated to reflux and stirred for 4 hours. The excess acid and THF was evaporated under vacuum, and the remaining slurry was taken up in water (100 ml) and ethyl acetate (100 ml), made basic with 50% sodium hydroxide, and extracted with ethyl acetate (3 X 125 ml). The organic layers were collected, dried (MgSO 4 ), filtered and concentrated. The crude product was purified on a silica gel column eluted with 10% methanol/methylene chloride. The product was collected and concentrated. The remaining solids were taken up in isopropyl alcohol (25 ml), and treated with saturated IPA/HCl (10 ml). The white solids were filtered and washed with isopropyl alcohol to give 4-(2-(((2- furanyl)methyl)amino)ethyl) nitrobenzene hydrochloride (2.9 g). (c) 4-f2-fff2-furanvnmethvnamino)ethvn aniline dihvdrochloride. To a stirred solution of the compound of step (b) (2.46 g) in acetic acid (100 ml) was added of zinc dust (3.3 g) in one portion. The mixture was stirred for 10 minutes. The zinc was filtered and the excess acid evaporated under vacuum. The remaining solids were taken up in water (100 ml) and ethyl acetate (100 ml), made basic with 50% sodium hydroxide, and extracted with ethyl acetate (3 X 125 ml). The organic layers were collected, dried (MgSO 4 ), filtered and concentrated. The remaining oil was taken up in isopropyl alcohol (25 ml) and treated with saturated IPA/HCl (10 ml). The white solids were filtered and washed with isopropyl alcohol to leave 4-(2-(((2-furanyl) methyl)amino)ethyl) aniline dihydrochloride (1.50 g). (d) N-f4-f2-fff2-Furanyl .methvnamino )ethvnphenvn-2-thiophenecarboximidamide dioxalate To a stirred suspension of the compound of step (c) (1.5 g) in DMF (15 ml) was added pyridine (0.42 ml) followed by of S-methyl-2-thiophenecarboxamide hydroiodide (the product of Example 1 step (d)) (1.51 g). The mixture was heated to 50 °C and stirred for 48 hours. The mixture was then diluted in 100 ml water and made basic with excess 50% sodium hydroxide. The crude product was extracted with ethyl acetate (3 X 100 ml). The organic layers were collected and washed with water (2 x 200 ml). The organic layer was dried (MgSO ), filtered and concentrated. The crude product was purified on a silica gel column eluted with 20% methanol/methylene chloride. The product was collected and concentrated to an oil, which was taken up in isopropyl alcohol and treated with 2.5 equivalents of oxalic acid. The white sohds were filtered and washed with ether to leave N-(4-(2- (((2-Furanyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide dioxalate (580 mg) m.p. decomposes >220 °C. Example 62 The following compounds were made following a process analogous to that of Example 61: (a) N-f4-f2-fff2-Pyridyl .methvnamino .ethvnphenvn-2-thiophenecarboximidamide trihvdrochloride. m.p. decomposes >250 °C. fb . N-f4-f2-fff2-Thiophenyl .methvnamino lethvnphenvn-2- thiophenecarboximidamide dioxalate. m.p. decomposes >226 °C. Example 63 N-f4-ffamino > )carbonvnphenyl>2-thiophenecarboximidamide hydroiodide This compound was prepared following the procedure of Example 26, step (b). The salt was recrystallized from 30% isopropanol in water, m.p. 236 °C (dec). Example 64 N-f4-fff2-thienvncarbonvnamino^phenyn-2-thiophenecarboximidamide oxalate (a) N-f4-nitrophenvn-2-thiophenecarboxamide This compound was prepared using the procedure described for Example 26, step (a), MS 249 (M+H). (b) N-f4-amino > laniline-2-thiophenecarboxamide This compound was prepared using the procedure described for Example 17, step (d). MS 219 (M=H). (c) N-f4-fff2-thienvncarbonvnamino phenyn-2-thiophenecarboximidamide oxalate This compound was prepared following the procedure of Example 7, step (c). The free base was converted to the oxalate salt in isopropanol, m.p. 231-3 °C. Example 65 N-[4-ffffDipheπylamino carbonyl .amino .methyl .phenyl]-2-thiophenecarboxamidine oxalate (a) 4-ffffDiphenylamino .carbonyl .amino .methyl . nitrobenzene To a stirred solution of 4-nitrobenzylamine hydrochloride (1.04 g, 5.51 mmol) and triethylamine (1.56 ml, 11.22 mmol) in methylene chloride (10 ml) was added diphenylcarbamyl chloride (1.40 g, 6.07 mmol). After stirring for 5 hr, the mixture was dumped into water and the layers separated. The aqueous layer was further extracted with methylene chloride (3 X 20 ml). The combined extracts were washed with water, dried (MgSO 4 ), filtered, and concentrated to yield a solid which was recrystallized from ethyl acetate/hexane/methanol to give 4-((((diphenylamino)carbonyl)amino)-methyl)nitrobenzene as a white solid: 1.37 g (72% yield); m.p. 137-138 °C. (b) 4-ffffDiphenylamino .carbonvnamino )methyl . aniline To a stirred solution of the compound of step (a) (1.37 g, 3.94 mmol) in THF/MeOH (100 ml, 1:1) was added a catalytic amount of 10% Pd/C. The mixture was hydrogenated at 50 psi for 1 hr, filtered through celite, and concentrated to give 4-((((diphenylamino)carbonyl)amino)methyl)aniline which was homogeneous by TLC and used immediately in the next reaction. (c) N-[4-ffffDiphenylamino carbonvnamino)methvnphenyl]-2-thiophenecarboxamidine oxalate To a solution of the compound of step (b) (1.24 g, 3.90 mmol) in isopropanol (10 ml) was added S-methyl-2-thiophenethiocarboximide hydroiodide (1.06 g, 3.70 mmol). The mixture was stirred for 18 hr, dumped into basic water and extracted with chloroform ( 3 X 30 ml). The combined extracts were washed with water, dried (MgSO 4 ), filtered, concentrated, and chromatographed over silica (6% methanol/methylene chloride) to an oil which solidified upon standing. A small amount was isolated as the oxalate salt: (48 mg); m.p. (dec) 150 °C. Example 66 N-f4-ffff2-thiophenyl .iminomethvnamino methvnphenvn-2-thiophenecarboxamidine difumarate a) 4-faminomethvnaniline To a solution of 4-nitrobenzylamine hydrochloride (9.0 g, 4.7 mmol) in methanol (200 ml) was added a catalytic amount of 10% palladium on carbon. The mixture was hydrogenated at 50 psi for 4 hours, filtered through celite, and concentrated to give a crude oil. The oil was dissolved in water (100 ml), and 20% sodium hydroxide (20 ml), was extracted twice with dichloromethane, the organic layer dried (MgSO 4 ), filtered and was concentrated to yield (6.1g) of 4- (aminomethyl)aniline. b) N-f4-ffff2-thiopheπvniminomethvnamino .methyl .phenyl .-2- thiophenecarboxamidine difumarate A mixture of the compound of step (a) (1.6 g 1.3 mmol) and S-methyl-2- thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (4.4 g, 1.5 mmol) in of DMF (10 ml) was warmed to 40 °C for 72 hours. The mixture was then diluted with 20% aqueous sodium hydroxide, and the solid collected by filtration to yield (2.5g) of crude N-(4-((((2- thiophenyl)iminomethyl)amino)methyl)phenyl)-2-thiophenecarboxamidine. The difumarate salt was made from methanol and isopropanol. m.p. 200-201 °C. Example 67 Following a process analogous to that of Example 66, the following compound was prepared: (a) N-f4-ffff2-thiophenvniminomethvDamino1ethyl ,pheπyl .-2- thiophenecarboxamidine difumarate m.p. 200-201 °C. Example 68 N-f4-ffff3-methylphenyl ,iminomethyl .amino .ethv1.phenvn-2- thiophenecarboxamidine difumarate a) N-f4-f2-aminoethyl .phenyl ,-2-thiophenecarboxamidine To a solution of 4-aminoethyl aniline dihydrochloride (1.4 g, 6.6 mmol) and S- methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)) (2.2 g, 7.9 mmol) in DMF (10 ml) was added pyridine (0.52 g, 6.6 mmol). The mixture was stirred at 40 °C for 24 hours, diluted with 20% aqueous sodium hydroxide, extracted twice with dichloromethane, dried (MgSO 4 ), filtered and concentrated to give N-(4-(2aminoethyl)phenyl)-2-thiophenecarboxamidine (4.1g) as an oil. b) N-f4-ffff3-methylphenyl minomethvDamino )ethvnphenvn-2- thiophenecarboxamidine difumarate To a solution of the compound of step (a) (2.0 g, 8.2 mmol) was added S-methyl-(3- methylphenyl)thiocarboximide hydroiodide (2.8 g, 9.2 mmol) in isopropanol (10 ml). The mixture was stirred at 40 °C for 18 hours, diluted with 20% aqueous sodium hydroxide, and extracted twice with ethyl acetate. The organic layer was washed with water (100 ml), dried (MgSO 4 ) filtered and concentrated to give an oil. The difumarate salt was made from methanol, isopropanol, and ethyl acetate to yield N- (4-((((3-methylphenyl)iminomethyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine difumarate: m.p. 200-201 °C. Example 69 Following a process analogous to that of Example 68, the following compound was prepared: (a) N-f3-ffff2-thiophenyl .iminomethvnamino . ethyl . phenyl ,-2- thiophenecarboxamidine difumarate. m.p. 211-212 °C. Example 70 N-f4-f2-ffpyrimidin-2-yl ,amino ethyl ,phenyl ,-2-thiophenecarboxamide dihvdrochloride a) [2-f4-nitrophenvnethyl]pyrimidi-2-ylamine To a solution of 4-nitrophenethylamine hydrochloride (2.0 g, 9.8 mmol) in dimethylformamide (20 ml) was added potassium carbonate (10 g) and 2- chloropyrimidine (1.6 g, 1.4 mmol). The mixture was heated to 100 °C for 24 hours, diluted with water (300ml), extracted twice with ethyl acetate, dried (MgSO ), filtered and concentrated to yield a crude solid. The monohydrochloride salt was made from ethyl acetate, isopropanol to yield (2.4g) of [2-(4-nitrophenyl)ethyl] pyrimidi-2-ylamine hydrochloride. b) [2-f4-aminophenyl .-ethyl]pyrimidi-2-ylamine To a solution of the compound of step (a) in acetic acid (100 ml) was added zinc dust (3.0 g). The reaction mixture was stirred for 30 minutes, filtered and concentrated. The residue was partitioned with 20% aqueous sodium hydroxide and dichloromethane and the organic layer was dried (MgSO ), filtered and concentrated to yield 1.3g of [2-(4-aminophenyl)-ethyl]pyrimidi-2-ylamine. c) N-f4-f2-ffpyrimidin-2-yl .amino)ethyl .phenylV2-thiophenecarhoxamide dihvdrochloride To a solution of the compound of step (b) (1.3 g, 6.6 mmol) in (10 ml) of dimethylformamide was added S-methyl-2-thiophenethiocarboximide hydroiodide (the product of Example 1, step (d)). The reaction was stirred for 72 hours, diluted with 20% sodium hydroxide and extracted twice with ethyl acetate. The combined extracts were washed twice with water, dried (MgSO 4 ), filtered and concentrated. The hydrochloride salt was made from methanol and isopropanol to yield N-(4-(2- ((pyrimidin-2-yl)amino)ethyl)phenyl)-2-thiophenecarboxamide dihydrochoride. m.p. 191-192 °C. Example 71 N-f4-f2-ffphenylmethvnamino .ethoxy)-2-fluoro-phenyl .-2-thiophenecarboximidamide a) 3-fluoro-4-nitrophenoxyacetic acid A sample of 3-fluoro-4-nitrophenol (5.18 g) and potassium carbonate (10 g) was treated with DMF (20 ml). Ethyl bromoacetate (5 ml) was then added, and the mixture was stirred at 22 °C. After two hours the mixture was treated with methanol (20 ml) and water (40 ml) and allowed to stir. After an additional two hours, the mixture was diluted with water to 200 ml and the solids filtered off. The filtrate was acidified and the solids collected to give 3-fluoro-4-nitrophenoxyacetic acid, m.p. 90-92 °C. b) N-phenylmethyl-3-fluoro-4-nitrophenoxyacetamide A sample of the compound of step (a) (2.84 g) was dissolved in dry THF (100 ml), then methylmorpholine (1.45 ml) was added and the mix stirred at 0 °C. Ethyl chloroformate, (1.26 ml) was added, and the mixture stirred for 2 minutes. Benzylamine (1.45 ml) was added, and the mixture as stirred at 0 °C. After 15 minutes the mixture was diluted slowly with water to 220 ml and stirred at 0 °C. The resulting solids were collected and washed with water then air dried to give N- phenylmethyl-3-fluoro-4-nitrophenoxyacetamide, m.p. 128.5-130 °C. (c) N-f4-f2-ffpheπylmethyl .amino)ethoxyV2-fluoro-phenyl .-2- thiophenecarboximidamide The product of step (b) was converted to N-(4-(2-((phenylmethyl)amino) ethoxy)-2-fluoro-phenyl)-2-thiophenecarboximidamide free base following a process analogous to that of Example 19, steps (c) and (d); m.p. 105-107 °C. Example 72 N-f4-f2-ffphenylmethvnfmethyl .amino .ethyl .phenyl .trifluoroacetimidamide 4-(2-((phenylmethyl)(methyl)amino)ethyl)aniline dihydrochloride (prepared following method of Example 19, steps (a)-(c)) (1.03 g) was dissolved in water (25 ml), treated with 50% sodium hydroxide (5 ml), and extracted with ethyl ether. The extract was dried (NaOH), and evaporated to provide free base, 0.72 g. The free base was then treated with trifluoroacetimidamide, and warmed to 100 °C. After one hour, toluene (5 ml) was added to improve stirring. The mixture was cooled after 2 hours, and water (30 ml) was added, and the mixture was stirred. After 15 minutes, the water was decanted from the resulting semisolid tan residue. The residue was recrystallised from a methanol and water mixture (125 ml), giving N-(4-(2-((phenylmethyl)(methyl)amino)ethyl)phenyl)-trifluoroacetimidamide, as a tan solid, m.p. 105-107 °C.

Claims: Claims 1. A compound of formula I wherein D represents phenyl, pyridinyl or a 5 membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which three groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen and perfluoroalkyl Cl to 6; or perfluoroalkyl Cl to 6; R 1 represents hydrogen, alkyl Cl to 6 or halogen; R 2 represents -X(CH 2 ) n ZCONR 3 R 4 , -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 , -X(CH 2 ) P NR 3 R 4 , -X(CH 2 ) n NHCOR s or -(CH 2 ) q NHC(NH)R 6 ; R 3 and R 4 independently represent hydrogen, alkyl Cl to 6, -(CH 2 )_A, -(CH 2 ) m OA or -CH(CH 3 )(CH 2 ) t A; or -NR 3 R 4 together represent 1-indanyl, piperonylamino-, piperidinyl, morpholinyl, pyrrolidinyl, 1,2,3,4-tetrahydroisoquinolinyl; or piperazinyl optionally 4-substituted by alkyl Cl to 6; R 5 represents alkyl Cl to 6, perfluoroalkyl Cl to 6, -(CH 2 ) or -O(CH 2 ).A; A represents phenyl, pyridinyl, pyrimidinyl, or a 5 membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which four groups are optionally substituted by one or more groups selected from alkyl Cl to 6, halogen, nitro, cyano and trifluoromethyl; R 6 represents phenyl, pyridinyl or a 5 membered heterocyclic aromatic ring containing 1 to 4 heteroatoms selected from O, S and N, which three groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen and perfluoroalkyl Cl to 6; or perfluoroalkyl Cl to 6; n and r independently represent an integer in the range 0 to 6 inclusive; p and w independently represent an integer in the range 1 to 5 inclusive; m represents an integer in the range 2 to 5 inclusive; q and t independently represent an integer in the range 0 to 5 inclusive; s represents an integer in the range 1 to 3 inclusive; X represents O or a bond; Z represents O, NR 7 or a bond; R 7 represents hydrogen or alkyl Cl to 6; provided that: (a) when D contains a heteroatom, it is not connected to the remainder of the compound of formula I through the heteroatom; (b) when R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and neither X nor Z represent a bond, then n represents an integer in the range 2 to 6 inclusive; (c) when R 2 represents -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 or -X(CH 2 ) n NHCOR 5 , and X represents O, then n represents an integer in the range 2 to 6 inclusive; (d) when R 2 represents -X(CH 2 ) p NR 3 R 4 and X represents O, then p represents an integer in the range 2 to 5 inclusive; (e) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D represents phenyl and R 6 represents phenyl, then q does not represent 0; (f) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D and R 6 represent 2-chlorophenyl, then q does not represent 0; (g) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D ,and R 6 represent 3-pyridinyl, then q does not represent 0; and (h) when R 2 represents -(CH 2 ) q NHC(NH)R 6 , R 1 represents hydrogen, D and R 6 represent 4-pyridinyl, then q does not represent 0; or a pharmaceutically acceptable salt thereof. 2. A compound as claimed in claim 1, wherein D represents phenyl, thiophene, pyrrole, furan or thiazole which five groups are optionally substituted by one or more groups selected from alkyl Cl to 6, alkoxy Cl to 6, halogen or perfluoroalkyl Cl to 6. 3. A compound as claimed in claim 1 or claim 2, wherein D represents thiophene, furan or pyrrole. 4. A compound as claimed in any one of the preceding claims wherein R 2 represents -X(CH 2 ) p NR 3 R 4 or -(CH 2 ) q NHC(NH)R 6 . 5. A compound as claimed in any one of the preceding claims wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , X represents a bond and either -NR 3 R 4 represents 1,2,3,4-tetrahydroisoquinolinyl or 1-indanyl or one of R 3 and R 4 represents -(CH 2 ),A and the other represents hydrogen or methyl. 6. A compound as claimed in any one of the preceding claims wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , p represents an integer in the range 1 to 3 inclusive, one of R 3 and R 4 represents -(CH 2 ).A and the other represents hydrogen or methyl, r represents 1 or 2 and A represents phenyl optionally substituted by one or more groups selected from alkyl Cl to 6 and halogen. 7. A compound as claimed in any one of claims 1 to 4 wherein R 2 represents -(CH 2 ) q NHC(NH)R°, q represents 0, 1 or 2 and R 6 represents phenyl or thiophene, which two groups are optionally substituted by one or more groups selected from alkyl Cl to 6 and halogen. 8. A compound as claimed in any one of claims 1 to 4 and claim 7 wherein R 2 represents -(CH 2 ) q NHC(NH)R 6 and q represents 0. 9. A compound of formula I, as claimed in claim 1, which is: N-(4-(2-((phenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(l-((phenylmethyl)amino)methyl)phenyl)-2-thiophenecarboximidamide; N-(4-(l-((phenethyl)amino)methyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((2-chlorophenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((3-fluorophenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((2-methylphenyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(methylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-aminoethyl)phenyl)-2-thiophenecarboximidamide; N-((4-morpholinylmethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(((phenylmethyl)amino)methyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((2,6-dichlorophenyl)methyl)amino)ethyl)phenyl)-2-thiophene carboximidamide; N-(4-(2-(((2-bromophenyl)methyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(3-(2-((Phenylmethyl)amino)ethyl)phenyl)-3-thiophenecarboximidamide; N-(4-(2-((2,6-dichlorophenylmethyl)amino)ethyl)phenyl) -3-thiophenecarboximidamide; N-(4-(2-aminoethyl)phenyl)-3-thiophenecarboximidamide dihydrobromide; N-(4-(2-((2,6-dichlorophenylmethyl)amino)ethyl)phenyl)-2-furanocarboximidamide; N-(3-(3-(l-pyrolidinyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-aminoethyl)phenyl)-2-furocarboximidamide dioxalate; N-(4-((l-piperidinyl)methyl)phenyl)-2-thiophenecarboximidamide; N-(4-((l-pyrrolidinyl)methyl)phenyl)-2-thiophenecarboximidamide; N-(3-(((phenylmethyl)amino)methyl)phenyl)-2-thiophenecarboximidamide; N-(3-((amino)methyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-((phenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(3-(2-(Ethylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(3-((Phenylethyl)amino)propyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(((2-Bromophenyl)methyl)amino)ethyl)phenyϊ)-2- thiophenecarboximidamide; N-(3-(2-(Phenylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(Ethylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(2-Propylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(l-Propylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(t-Butylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(n-Butylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(Methylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(l-Propylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(t-Butylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(2-Propylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-aminoethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(Dimethylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(Diethylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-(2-(l,2,3,4-Tetrahydro)isoquinolinyl)ethyl)phenyl)-2- thiophenecarboximidamide; N-(4-(3-(2-(l,2,3,4,-tetrahydro)isoquinolyl)propyl)phenyl)-2-thiophene carboximidamide; N-(3-(2-(((2-Chlorophenyl)methyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(3-(3-((Phenylmethyl)amino)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((3-Chlorophenyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(4-(2-(((4-Chlorophenyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(4-(2-(((3-Chlorophenyl)methyl)amino)ethyl)phenyl)-3-chlorothiophene- 2-carboximidamide; N-(3-(2-((N-Phenylmethyl-N-methyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(4-(2-((N-Phenylmethyl-N-methyl)amino)ethyl)phenyl)-2-thiophene carboximidamide; N-(3-(2-(((3-Chlorophenyl)methyl)amino)ethyl)phenyl)-2-thiophene carboximidamide; N-(3-(2-(((3-fluorophenyl)methyl)amino)ethyl)phenyl)-2-thiophene carboximidamide; N-(4-(3-(((3-Chlorophenyl)methyl)amino)propyl)phenyl)-2-thiophene carboximidamide; N-(4-(3-((phenylmethyl-N-methyl)amino)propyl)phenyl)-2-thiophene carboximidamide; N-(4-(3-((phenylamino)carbonyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(3-((phenylmethylamino)carbonyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(3-((l-pyrrolidyl)carbonyl)propyloxy)phenyl)-2-thiophenecarboximidamide; N-(4-(3-((4-mo holinyl)carbonyl)propyloxy)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((phenylmethylamino)carbonyl)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-((phenylamino)carbonyl)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(3-((phenylan_ino)carbonyl)propyl)phenyl)-2-pyrrolecarboximidamide; N-(4-(3-((phenylamino)carbonyl)propyl)phenyl)-2-furocarboximidamide; N-(4-(3-((phenylamino)carbonyl)propyl)phenyl)-3-chloro-2- thiophenecarboximidamide; N-((3-((phenylamino)carbonyl)propyl)phenyl)-l-methylpyrrole-2-carboximidamide; N-(4-(3-(l-(4-methylpiper_-zinyl)carbonyl)propyl)phenyl)-2-thiophene carboximidamide; N-(4-(3-((l-pyrrolidyl)carbonyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(3-((4-morphohnyl)carbonyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-((phenylamino)carbonyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((4-morpholinyl)carbonyl)ethyl)phenyl)-2-methylthiazole-4-carboximidamide; N-(4-(2-(((4-morpolinyl)carbonyl)amino)ethyl)phenyl)thiophene-2-carboximidamide ; N-(4-(3-(phenylaminocarbonyl)propyloxy)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((trifluoromethyl)carbonyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(4-(2-(((methyl)carbonyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((phenylmethyl)carbonyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((phenyl)carbonyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-((phenyliminocarbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-N"-( l,4-phenylene)bis-2-thiophenecarboximidamide; N-N"-(l,3-phenylene)bis-2-thiophenecarboximidamide; N,N'-(l,3-phenylene)bis-2-chlorophenylcarboximidamide; N,N'-(l,4-phenylene)bis-3-chlorothiophene-2-carboximidamide; N-(4-(((2-methoxyphenyl)iminocarbonyl)amino)phenyl)-2-thiophene carboximidamide; N-(4-(((Phenyl)iminocarbonyl)amino)phenyl)-3-chlorothiophene-2-carboximidamide; N-(4-(((Phenyl)iminocarbonyl)amino)phenyl)-3-thiophenecarboximidamide; N-(3-(((phenyl)iminocarbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-(4-(((4-chlorophenyl)iminocarbonyl)amino)phenyl)-2-thiophenecarboximidamine; N-(4-(((2-chlorophenyl)iminocarbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-(4-(((4-bromophenyl)iπn^ocarbonyl)arj__ino)phenyl)-2-thiophenecarboximidamide; N-(4-(((3-chloro-4-methylphenyl)iminocarbonyl)amino)phenyl)-2-thiophene carboximidamide; N-(4-(((3,5-dimethoxyphenyl)iminocarbonyl)amino)phenyl)-2- thiophenecarboximidamide; N-(4-(((3,5-dichlorophenyl)iminocarbonyl)amino)phenyl)-2- thiophenecarboximidamide; N-(4-(((Phenyl)immocarbonyl)amino)phenyl)-2-furancarboximidamide; N-(4-(((3-methylphenyl)iminocarbonyl)amino)phenyl)-2- thiophenecarboximidamide; N-(4-(((3-Methoxyphenyl)iminocarbonyl)amino)phenyl)-2-thiophene carboximidamide; N-(4-(((3-Bromophenyl)immocarbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-(4-(((3-chlorophenyl)iminocarbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-(4-(((3-methylphenyl)immocarbonyl)amino)phenyl)-2-pyrrolecarboximidamide; N-(4-(((4-chlorophenyl)iminocarbonyl)amino)phenyl)-2-pyrrolecarboximidamide; N-(4-(2-(((Phenylamino)carbonyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(4-(2-(((phenylamino)carbonyl)o3_y)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-((bis(phenylmethyl)amino)methyl)phenyl)-2-thiophenecarboxiπ-idamide; N-(4-(2-aminomethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(l,2,3,4-tetrahydroisoquinolin-2-ylmethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(4-((phenylmethyl)amino)butyl)phenyl)-2-thiophenecarboximidamide; N-(4-((((2-thiophenyl)iminomethyl)amino)methyl)phenyl)-2- thiophenecarboximidamide; N-(3-(3-( l-pyιτolidinyl)propyl)phenyl)-phenylcarboximidamide; N-(4-(2-((4-methoxyphenylmethyl)amino)ethyl)phenyl) -2-thiophenecarboximidamide; N-(4-(2-((4-methylphenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-((3-phenylpropyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-((2-methylphenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-[4-(2-((l-indanyl)ethyl)amino)phenyl]-2-thiophenecarboximidamide; N-(4-(2-(((4-pyridyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((2-thienyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-((2-phenylethyl)ammo)ethyl)phenyl)-2-thiophenecarboximidan__ide; N-(4-(2-aminoethyl)phenyl)-2-py_τolecarboximidamide; (S)-N-(4-(2-((l-phenylethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; (R)-N-(4-(2-((l-phenylethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(3-(2-((4-phenylbutyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(((phenylmethoxy)carbonyl)aminomethyl)phenyl)-2-thiophene carboximidamide; N-(4-(2-((phenylmethyl)amino)ethoxy)phenyl)-2-thiophenecarboximidamide; N-(4-(((diphenylamino)carbonyl)amino)phenyl)-2-thiophenecarboxamidine; N-(3-((benzoyl)amino)phenyl)-2-thiophenecarboximidamide; N-(4-((benzoyl)amino)phenyl)-2-thiophenecarboximidamide; N-(3-(((phenylamino)carbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-(3-(((4-phenoxylbutyl)amino)carbonyl)phenyl)-2-thiophenecarboximidamide; N-(3-(((4-phenylbutyl)amino)carbonyl)phenyl)-2-thiophenecarboximidamide; N-(4-((((benzyl)amino)carbonyl)methyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(l-pyrrolidinyl)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(l-piperidinyl)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(3-(l-pyιτolidinyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(3-(l-piperidinyl)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(2-(l,2,3,4-tetrahydro)isoquinolyl)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-((((phenylmethyl)amino)methylcarbonyl)amino)phenyl)-2- thiophenecarboximidamide; N-(4-(2-(((2-furanyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((2-Pyridyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(((2-Thiophenyl)methyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-3-((aminocarbonyl)phenyl)-2-thiophenecarboximidamide; N-(4-(((2-thienyl)carbonyl)amino)phenyl)-2-thiophenecarboximidamide; N-[4-((((Diphenylamino)carbonyl)an_ano)methyl)phenyl]-2-thiophenecarboxamidine; N-(4-((((2-thiophenyl)iminomethyl)amino)methyl)phenyl)-2- thiophenecarboxamidine; N-(4-((((2-thiophenyl)iminomethyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(4-((((3-methylphenyl)immomethyl)amino)ethyl)phenyl)-2- thiophenecarboxamidine; N-(3-((((2-thiophenyl)iminomethyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(4-(2-((pyrimidin-2-yl)amino)ethyl)phenyl)-2-thiophenecarboxamide; N-(4-(2-((phenylmethyl)amino)ethoxy)-2-fluoro-phenyl)-2-thiophene carboximidamide; N-(4-(2-((ρhenylmethyl)(methyl)amino)ethyl)phenyl)trifluoroacetimidamide; N-(4-(2-((ethyl)(phenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((propyl)(phenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((l,l-dimethylethyl)(phenylmethyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(4-(2-((3,4-dichlorophenylmethyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(4-(2-((3,5-bistrifluoromethylphenylmethyl)amino)ethyl)phenyl)-2- thiophenecarboximidamide; N-(4-(3-(ethylamino)propyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-(diethylamino)ethyl)phenyl)-2-thiophenecarboximidamide; N-(4-(2-((3-chlorophenylmethyl)amino)ethyl)phenyl)-benzenecarboximidamide; N-(4-(2-((3-chlorophenylmethyl)amino)ethyl)phenyl)-3-chlorothiophene-2- carboximidamide; N-(4-(2-((4-methylphenylmethyl)amino)ethyl)phenyl)-2-thiophenecarboxamidine; N-(4-(2-(piperonylamino)ethyl)phenyl)-2-thiophenecarboxamidine; or a pharmaceutically acceptable salt of any one thereof. 10. A pharmaceutical formulation including a compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, in admixture with a pharmaceutically acceptable diluent or carrier. 11. A compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, for use as a pharmaceutical. 12. Use of a compound of formula I, as defined in claim 1, without provisos (e) to (h), or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment of neurodegenerative disorders or of migraine or for the prevention and reversal of tolerance to opiates and diazepines or for the treatment of drug addiction. 13. A method of treatment of neurodegenerative disorders or of migraine or of tolerance to opiates and diazepines or of drug addiction which comprises administering a therapeutically effective amount of a compound of formula I, as defined in claim 1, without provisos (e) to (h), or a pharmaceutically acceptable salt thereof, to a person suffering from such a condition. 14. A process for the preparation of a compound of formula I, as defined in claim 1, or a pharmaceutically acceptable salt thereof, which comprises: (a) preparing a compound of formula I, by reacting a corresponding compound of formula II wherein D is as defined in claim 1 and L is a leaving group, with a compound of formula III wherein R 1 and R 2 are as defined in claim 1, (b) preparing a compound of formula I, by reacting a corresponding compound of formula IV IN I V wherein D is as defined in claim 1, with a compound of formula V wherein R 1 and R 2 are as defined in claim 1 and HA is an acid, (c) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 , -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 or -X(CH 2 ) p NR 3 R 4 and at least one of R 3 and R 4 represents alkyl Cl to 6, -(CH 2 )_A, -(CH 2 ) m OA or -CH(CH 3 )(CH 2 ),A by reacting a corresponding compound of formula I in which one or both of R 3 and R 4 represents hydrogen with a compound of formula VI, R 8 - L V I wherein R 8 represents alkyl Cl to 6, -(CH 2 )Λ -(CH 2 ) m OA or -CH(CH 3 )(CH 2 ) t A and L is a leaving group, (d) preparing a compound of formula I in which R 2 represents -(CH 2 ) q NHC(NH)R 6 by reacting a corresponding compound of formula VII wherein D, R 1 and q are as defined in claim 1, with a compound of formula VIII N H A R s wherein R 6 is as defined in claim 1 and L is a leaving group, (e) preparing a compound of formula I in which R 2 represents -(CH 2 ) q NHC(NH)R 6 by reacting a corresponding compound of formula IX wherein D, R 1 and q are as defined in claim 1 and HA is an acid, with a compound of formula X wherein R 6 is as defined in claim 1, (f) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 by reacting a corresponding compound of formula XI, wherein D, R 1 , X, n, Z and L are as defined in claim 1, with a compound of formula XII, R 3 R 4 NH X I I wherein R 3 and R 4 are as defined in claim 1, (g) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n NHCO(CH 2 ) s NR 3 R 4 , by reacting a compound of formula XIII H 2 N( wherein D, R 1 , X and n are as defined in claim 1, with a compound of formula XIV R ,3 5 B * 4 N(CH 2 ),C0L XIV wherein R 3 , R and s are as defined in claim 1 and L is a leaving group, (h) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n NHCOR 5 , by reacting a compound of formula XIII with a compound of formula XV R 5 C 0 L X V wherein R 5 is as defined in claim 1 and L is a leaving group, (i) preparing a compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and Z represents NR 7 by reacting a corresponding compound of formula I in which R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and Z represents -NH with a compound of formula XVI R 7 - L X V I wherein R 7 is as defined in claim 1 and L is a leaving group, (j) preparing a compound of formula I in which R 2 represents -X(CH 2 ) p NR 3 R 4 , and p is not less than 2, by reduction of a compound of formula XVII wherein D, X, R\ R 3 , R 4 and p are as defined in claim 1, (k) preparation of a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 and both R 3 and R 4 represent hydrogen, by reduction of a corresponding compound of formula XVIII wherein R\ D, p and X are as defined in claim 1, (1) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) n ZCONR 3 R\ Z represents O or NR 7 and R 3 represents hydrogen by reacting a compound of formula XIX HZ( wherein R\ D, X and n are as defined in claim 1 and Z represents O or NR 7 , with a compound of formula XX R 4 -N=C=0 XX wherein R 4 is as defined in claim 1, (m) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) n NHCOR s and R 5 represents -O(CH 2 ) . A by reacting a compound of formula XXI wherein R 1 , D, X and n are as defined in claim 1, with a compound of formula XXII A ( CH 2 ) w 0H XX I I wherein A and w are as defined in claim 1, (n) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) n ZCONR 3 R 4 and Z represents O or NR 7 by reacting a compound of formula XIX with a compound of formula XXIII wherein R 3 and R 4 are as defined in claim 1, (o) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , R 3 represents hydrogen and p represents an integer 2 to 5, by reduction of a compound of formula XXTV wherein R 1 , R 4 , D, X and p are as defined in claim 1, (p) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 ) A in which r represents an integer 2 to 6, by reduction of a compound of formula XXV XXV wherein R 1 , A, D, r and p are as defined in claim 1, (q) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 ) m OA, by reduction of a compound of formula XXVI A°< CH _)m-,-< * H- N -( wherein R 1 , A, D, p and m are as defined in claim 1, (r) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 )A in which r represents an integer 2 to 6, by reduction of a compound of formula XXVII A(CH 2 ) r _,C0NH(C X X V wherein R 1 , A, D, p and r are as defined in claim 1, or (s) preparing a compound of formula I wherein R 2 represents -X(CH 2 ) p NR 3 R 4 , one of R 3 and R 4 represents hydrogen, and the other represents -(CH 2 ) m OA, by reduction of a compound of formula XXVTII wherein R 1 , A, D, p and m are as defined in claim 1, and where desired or necessary converting the resultant compound of formula I, or another salt thereof, to a pharmaceutically acceptable salt thereof, or vice versa.

Inventors: GENTILE ROBERT JAMES, MACDONALD JAMES EDWIN, MURRAY ROBERT JOHN, SHAKESPEARE WILLIAM CALVIN, GENTILE, ROBERT, JAMES, MACDONALD, JAMES, EDWIN, MURRAY, ROBERT, JOHN, SHAKESPEARE, WILLIAM, CALVIN
Applicants: FISONS CORP, FISONS PLC, GENTILE ROBERT JAMES, MACDONALD JAMES EDWIN, MURRAY ROBERT JOHN, SHAKESPEARE WILLIAM CALVIN, FISONS CORPORATION, GENTILE, ROBERT, JAMES, MACDONALD, JAMES, EDWIN, MURRAY, ROBERT, JOHN, SHAKESPEARE, WILLIAM, CALVIN
Application year: 1994
Main classification: C07D207
IPC codes: A61K31, A61P1, A61P3, A61P7, A61P9, A61P25, A61P29, A61P31, A61P37, A61P43, C07C257, C07D207, C07D295, C07D307, C07D333, C07D409