Carbazolone derivatives and process for preparing the same

Abstract: The invention relates to carbazolone derivatives of the formula wherein A stands for a group of formula -CH 2 -R wherein R means a hydroxyl or 2-methyl-1H-imidazol-1-yl group; B represents a group of formula wherein R 1 means hydrogen or a methyl or ethyl group; or A and B together form a group of formula wherein R 2 means a methyl or ethyl group; or A and B together form a group of formula The above compounds are useful intermediates in the synthesis of ondansetron of formula chemically 9-methyl-3-[(2-methyl-1H-imidazol-1-yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one. The invention further relates to a novel process for the preparation of compounds of the formula (I), wherein A and B are the same as in formula (I).

Description: The invention relates to novel carbazolone derivatives of formula wherein A stands for a group of formula -CH 2 -R wherein R means a 2-methyl-1H-imidazol-1-yl group; B represents a group of formula wherein R 1 means hydrogen or A and B together form a group of formula wherein R 2 means an ethyl group; or A and B together form a group of formula Furthermore, the invention relates to a process for the preparation of the above compounds. The compounds of formula (I) are valuable intermediates in the synthesis of 9-methyl-3-[(2-methyl-1H-imidazol-1-yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one of formula (generic name: ondansetron) and its acid addition salts, preferably the hydrochloride dihydrate. Thus, the invention relates also to a novel process for the preparation of ondansetron of the formula (II). Due to its selective 5-HT 3 antagonistic effect, ondansetron is an excellent antiemetic drug, inhibiting vomiting and nausea by decreasing the gastric motility, mainly during cancer chemotherapy (see GB patent specification No. 2,153,821). Several processes have been published for the preparation of ondansetron and also for its 3-alkylated derivatives (GB-A-2 192 885). According to a process described in the published European patent application No. 219,929, the imidazolyl-alkyl side chain is introduced by a three-step synthesis onto the methylene group neighbouring the oxo group of the 1,3-cyclohexanedione monoenol ether used as starting substance. After that the enol ether group is replaced by a 2-methyl-2-phenylhydrazino group and the phenylhydrazone obtained is subjected to a Fischer's indole synthesis. The drawbacks of this process, comprising five steps, reside in the use of dangerous and expensive reagents (butyl lithium, dimethylmethyleneammonium iodide, 1-methyl-1-phenylhydrazine) and technological steps, which result a moderate yield and which are difficult to carry out, in some cases, and to increase to industrial scales (c.f. column chromatography, temperature of -70°C). The overall yield of ondansetron is only 9.57%, calculated for 1,3--cyclohexanedione monoenol ether. According to another method described in the published European patent application No. 221,629, after reacting an imidazolylalkyl-1,3-cyclohexanedione monoenol ether with 2--iodoaniline, the enamine obtained is cyclized with palladium(II) acetate. The indole-N atom of the product thus formed is methylated in the last step. The drawbacks of this process are the same as those of the former one. The overall yield of ondansetron is at most 1.0%, calculated for 1,3-cyclohexanedione monoenol ether used as starting substance. According to the above-said GB patent specification No. 2,153,821 (which is equivalent to the Hungarian patent specification No. 193,592), and also according to the European Patent Application EP-A-0 219 193 3-(dimethylaminomethyl)-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one is used as starting substance, which is heated with 2-methylimidazole to obtain ondansetron. Although neither of these specifications disclose the preparation and characterization of the starting tertiary amine compounds, it would seem possible to obtain these according to the method disclosed in G.R. Allen, et al, J. Med. Chem. 15 (1972) 875. Peculiarly, the above-said patent specification indicates this tertiary amine to be known per se and, in addition, ondansetron can be prepared therefrom in an other way, too. After quaternizing the tertiary amine with methyl iodide, trimethylamine is split off from the obtained metho-iodide by Hofmann's elimination reaction to give 9-methyl-3-methylene-1,2,3,9-tetrahydro-4H-carbazol-4-one. The thus-obtained electrophilic conjugated enone is subjected to an addition reaction with 2-methylimidazole (see Example 8 of the specification). The surprisingly moderate yield of 43.2% of the reaction shows that the significance of this simple direct addition, starting from the separated enone, should not be overestimated. However, the reaction of 2-methylimidazole with the said tertiary amine compound (c.f. Example 7) results in a good yield of ondansetron (100% of crude product, 82% of recrystallized product). These data suggest that the reaction mechanism of the last step in the known synthesis of ondansetron is decisively not an elimination-addition reaction in its character but has another type, i.e. N,N'-transamination by direct substitution. The present invention is aimed at developing a preparation process, in the course of which a pure final product can be obtained from novel intermediates through selective reactions, being easy to carry out and increase to an industrial scale, whereby the above drawbacks can be eliminated. The invention is based on the surprising discovery that any of the new alkoxyoxalylated 4-carbazolones of formula can N-monoalkylate 2-methylimidazole of formula and the obtained intermediate of formula chemically 9-methyl-3-[(2-methyl-1H-imidazol-1-yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid, can be converted into ondansetron of the formula (II) by dealkoxyoxalylating with a nucleophilic reagent. The surprising character of this solution can be explained as follows: It is well-known that the N-acylation of imidazoles can easily be carried out in a non-aqueous medium by using strong acylating agents, e.g. reactive esters (Alan E. Katritzky, Charles W. Rees: "Comprehensive Heterocyclic Chemistry", Vol. 5, p. 390-393). Based on this one could expect that the lactone of the formula (Ic), being a strong acylating agent, would acylate 2-methylimidazole of the formula (IV) to give the following compound: In an entirely surprising manner the methylene part of the C-hydroxymethyl group was attached to the nitrogen atom of 2-methylimidazole in an N-alkylation reaction, giving the compound of the formula (Id), being sterically more dense, rather than the N-acylimidazole of the formula ("IX"). Thus, the present invention relates to novel compounds of formula wherein A stands for a group of formula -CH 2 -R wherein R means a 2-methyl-1H-imidazol-1-yl group; B represents a group of formula wherein R 1 means hydrogen or A and B together form a group of formula wherein R 2 means an ethyl group; or A and B together form a group of formula The new compounds are as follows: 3-ethoxyoxalyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one, 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid lactone and 9-methyl-3-[(2-methyl-1H-imidazol-1 -yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid. Further, the invention relates also to a process for the preparation of compounds of formula wherein A stands for a group of formula -CH 2 -R wherein R means a 2-methyl-1H-imidazol-1-yl group; B represents hydrogen or a group of formula wherein R 1 means hydrogen ; or A and B together form a group of formula wherein R 2 means an ethyl group: or A and B together form a group of formula which comprises a) reacting the ketone of formula with a di(C 1-2 alkyl) oxalate in the presence of a basic agent in order to obtain a novel compound of the formula wherein R 2 stands for an ethyl group; b) reacting a compound of the formula (la), wherein R 2 is as defined in step a) above with formaldehyde in the presence of a basic catalyst in an aprotic solvent in order to obtain the novel compound of the formula (Ic); c) reacting a compound of the formula (Ic) with 2-methylimidazole of the formula (IV) in order to obtain the novel compound of the formula (Id); d) reacting the compound of the formula (Id) with a base, preferably an alkali metal carbonate or hydroxide, in order to obtain ondansetron of the formula (II), and, if desired, converting ondansetron of the formula (II) into its pharmaceutically acceptable acid addition salt. According to variant b) of the above process, the compounds of formula (la), wherein R 2 is as defined above, are reacted with 1 to 2 moles, preferably 1.2 to 1.6 moles, of formaldehyde in the presence of not more than 0.2 mole of a basic catalyst, preferably an alkaline metal carbonate or a trialkyl amine. If the compound of the formula (Ic) is to be prepared, the reaction with formaldehyde is carried out preferably in a dipolar aprotic solvent like acetonitrile or acetone. When in variant c) of the process of the invention a compound of the formula (Ic) is used as starting substance, the oxalyl group is removed by alcoholysis of the C-C bond in the presence of a C 1-4 alkanol and the group cleaved off is bound by salt formation with a base being stronger than 2-methylimidazole, preferably with triethylamine. 2-Methylimidazole is used in an amount of 1.0 to 3.0 moles, preferably 1.5 to 2.0 moles, calculated for the compound of the formula (Ic). When carrying out variant c) of the process of the invention, a compound of the formula (Ic) is heated with 2-methylimidazole in an aprotic solvent until the reaction becomes complete. Then in variant d) the oxalyl group is removed by a nucleophilic agent to obtain the target compound of the formula (II). According to a preferred embodiment of variant c) of the process of the invention, 1.0 to 3.0 moles, preferably 1.5 to 2.0 moles of 2-methylimidazole, 1.0 to 2.0 moles of ethanol and a base stronger than 2-methylimidazole, advantageously 1.1 to 1.5 moles of triethylamine, each calculated for 1 mole of a compound of the formula (Ic), are used in an ether-type solvent such as dioxane, or in a dipolar aprotic solvent, preferably dimethylformamide, dimethylsulfoxide or sulfolane. The reaction requiring heating is carried out at a temperature between 70°C and 200°C, preferably between 100°C and 150°C, for 0.25 to 20 hours, advantageously 0.25 to 5 hours, then the product of the formula (II) is precipitated by dilution with water or by salt formation and isolated by filtration. The compound of the formula (II) is obtained from the compound of the formula (Id) by using an aqueous alkaline metal hydroxide or alkaline metal carbonate solution at a temperature from 20°C to 100°C, preferably a potassium hydroxide or carbonate solution at 30 to 70°C. The compound of the formula (II) obtained in the free base form may be converted into its monohydrochloride dihydrate in a way known per se. In comparison to the processes known in the art the advantages of the process according to the invention are as follows. a) The process consists of reactions easy to carry out and to increase up to industrial scales. b) The alkoxyoxalyl group proves to be an excellent adjuvant function for the selective introduction of the imidazolylmethyl side chain, which is later easily split off in the form of an oxalate salt, in some cases spontaneously, in situ in the reaction mixture. c) The reactions can be performed in very good yields of 70 to 90% to result in well-isolable and well-characterizable crystalline substances in all cases. d) An additional advantage appears also therein that the intermediates of the reaction sequence do not contain any basic group; therefore, the final product is easy to purify. Thus, the isolation of a pure final product becomes extremely simple since none of the possible or actual impurities contains a basic group. In contrast, the synthesis disclosed in the above-cited GB patent specification No. 2,153,821 proceeds through the intermediate 3-(dimethylaminomethyl)-carbazol-4-one, i.e. through a substance basic in its character and difficult to remove. The invention is illustrated in detail by the following Examples. Example 1 Preparation of 3-ethoxyoxalyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one [compound of formula (la), wherein R 2 means an ethyl group] 3.0 g (0.13 mole) of sodium metal are portionwise added to a stirred mixture containing 19.93 g (0.1 mole) of 9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one of formula (III), 19.0 g (0.13 mole) of diethyl oxalate, 2 g of ethanol and 200 ml of dioxane. The slightly warming reaction mixture is stirred at 40 to 50°C for 4 hours, then 16 g of glacial acetic acid and finally 200 ml of water are added thereto at room temperature. After filtering off the yellow crystalline suspension, the precipitate is washed with water and dried to give the title compound in a yield of 24 g (80.2%), m.p.: 118-120°C. The active ingredient content of the product amounts to 98.4% based on potentiometric titration with sodium hydroxide solution. IR spectrum (KBr), ν max : OH 3600-2000 cm -1 C=0 (ester) 1727 cm -1 0=C-C=C 1590 cm -1 1578 cm -1 C-0-C (ester) 1213 cm -1 =C-OH (enol) 1185 cm -1 Ar-H (bending) 754 cm -1 1 H-NMR (DMSO-d6) δ ppm: CH 3 -CH 2 - 1.47 (3H, t) -CH 2 - 2.75 (2H, t) -CH 2 - 3,12 (2H, t) CH 3 -N 3.60 (3H, s) CH 3 - CH 2 -O 4.36 (2H, q) Ar-H 7.24 (2H, m) 8.00 (1H, dd) 8.13 (1H, dd) Example 2 Preparation of 3-ethoxyoxalyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one [compound of formula (la), wherein R 2 means an ethyl group] After adding 7.1 g (0.13 mole) of solid sodium methoxide to a stirred mixture containing 19.93 g of the ketone of formula (III) (for the chemical name see Example 1), 19 g of diethyl oxalate and 200 ml of 1,2-dimethoxyethane, the process described in Example 1 is followed to obtain 23.1 g (77.2%) of the title compound, m.p.: 117-120°C. The titrimetrically determined active agent content of the product is 97.9%. The spectroscopical data of the product are the same as described in Example 1. Example 3 Preparation of 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid lactone [compound of formula (Ic)] After adding 0.1 g of triethylamine to a stirred suspension containing 3.00 g (0.01 mole) of the ethoxyoxalyl compound of the formula (la), wherein R 2 means an ethyl group (for the chemical name see Example 1) in 20 ml of acetone, 1.13 g (0.015 mole) of formol solution are dropwise added to the mixture. The suspension becomes clear within 1 to 2 minutes and crystals begin to precipitate. After further stirring at 35 to 40°C for one hour, the reaction mixture is cooled down to room temperature, filtered off, the precipitate is washed with 50% acetone and dried to give 2.10 g (74.2%) of the title compound, m.p.: 242-244°C. IR spectrum (KBr),ν max . O-H 1794 cm -1 (lactone) C=0 1782 cm -1 (alpha-oxo) C=0 1642 cm -1 (carbazol-4-one) C-0-C 1259 cm -1 Ar (skeleton vibration) 1579 cm -1 Ar-H (bending) 755 cm -1 1 H-NMR (DMSO-d6) δ ppm: -CH 2 - 2.4 (1H, m) 2.75 (1H, m) -CH 2 - 3.09 (1H, m) 3.78 (1H, m) CH 3 -N- 3.75 (3H, s) -C-CH 2 O 4.53 (1H, d) 5.04 (1H, d) Ar-H 7.22 (2H, m) 7.53 (1H, dd) 7.92 (1H, dd) Example 4 Preparation of 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid lactone [compound of formula (Ic)] To a suspension containing 29.93 g (0.10 mole) of 3-ethoxyoxalyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one and 10.5 g (0.14 mole) of formol solution in 200 ml of acetonitrile, 1.0 g (0.0072 mole) of potassium carbonate is added. The reaction mixture is stirred at 30 to 35°C for one hour. Subsequently, the process described in Example 3 is followed to obtain 22.46 g (75.04%) of the title compound, m.p.: 240-243°C. The spectroscopic data of the product are identical with those of the product of Example 3. Example 5 Preparation of ondansetron base (chemically 9-methyl-3-[(2-methyl-1H-imidazol-1-yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one) A mixture containing 2.83 g (0.01 mole) of 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid lactone of the formula (Ic), 15 ml of dioxane, 1.32 g of triethylamine, 1.0 g of ethanol and 1.64 g (0.02 mole) of 2-methylimidazole is boiled under reflux while stirring for 5 hours. Thereafter, the reaction mixture is diluted with 45 ml of water and cooled down. The precipitate is filtered off, washed with aqueous dioxane and dried to obtain 2.56 g (87.3%) of the title compound, m.p.: 220-223°C. IR spectrum (KBr), ν max : C=0 1623 cm -1 Ar (skeleton) 1579 cm -1 N-CH 3 1483 cm -1 1460 cm -1 HetAr-H (bending) 781 cm -1 Ar-H (bending) 758 cm -1 1 H-NMR (DMSO-d6) δ ppm : -CH 2 - 1.98 (1H, a, axial) 2.17 (1H, e, equatorial) CH 3 -C 2.67 (3H, s) -CH 2 - 2.94 (1H, a) 3.11 (1H, e) -CH- 3.10 (1H, m) CH 3 -N 3.68 (3H, s) -CH-CH 2 -N 4.30 91H, dd) 4.68 (1H, dd) Ar-H 7.25 (2H, m) 7.50 (1H, dd) 8.03 (1H, dd) HetAr-H 7.57 (1H, d) 7.67 (1H, d) Example 6 Preparation of ondansetron base a) Preparation of 9-methyl-3-[(2-methyl-1 H-imidazol-1-yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one-qlyoxylic acid [compound of formula (Id)] A mixture containing 2.83 g (0.01 mole) of 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid lactone [compound of the formula (Ic)] and 1.64 g (0.02 mole) of 2-methylimidazole in 6.0 ml of sulfolane (tetramethylenesulfone) is heated in an oil bath of 150 to 160°C for 15 minutes while stirring. After cooling down and diluting with 60 ml of acetone the precipitate is filtered off, washed with acetone and dried to give 0.95 g of the title compound, m.p.: 190-200°C (with decomposition). The active agent content of this product measured by titration with perchloric acid in glacial acetic acid was found to be 96%. In the combined filtrate of the reaction, ondansetron could be detected by thin layer chromatography. The most important characteristics of the title product are as follows: IR spectrum (KBr), ν max : X-H 3440 cm -1 2650 cm -1 2550 cm -1 1973 cm -1 C=0 1628 cm -1 (alpha-oxo + carbazol-4-one) -COO- 1595 cm -1 Ar-H (bending) 763 cm -1 b) Preparation of ondansetron base A suspension containing 0.73 g (0.002 mole) of the product of the formula (Id) prepared in the preceding step a) and 0.40 g (0.0061 mole) of 85% potassium hydroxide in 20 ml of water is stirred at 45 to 50°C for one hour. After cooling down and filtering off the suspension, the precipitate is thoroughly washed with water and dried to give 0.50 g (85.32%) of the title product, m.p.: 223-225°C. The spectroscopic data of the product are in agreement with those of the product of Example 5. The active agent content of the product was found to be 97.6% based on the potentiometric titration with hydrochloric acid. Example 7 Preparation of 9-methyl-3-[(2-methyl-1H-imidazol-1-yl)-methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one hydrochloride dihydrate The process described in Example 5 is followed, except that after cooling down the reaction mixture to room temperature after boiling, 20 ml of 37% aqueous hydrochloric acid are added thereto. Then, the precipitate is filtered off, washed with isopropanol and dried to obtain 2.40 g (65.6%) of the title salt, m.p.: 178-180°C. The active agent content of the product was found to be 100.3% based on potentiometric titration with sodium hydroxide solution. The theoretical water content is 9.85% (calculated for C 18 H 19 N 3 O·HCl·2H 2 O). The water content measured is 10.03%.

Claims: Novel compounds of the formula wherein A stands for a group of formula -CH 2 -R wherein R means a hydroxyl or 2-methyl-1H-imidazol-1-yl group; B represents a group of formula wherein R 1 means hydrogen or a methyl or ethyl group; or A and B together form a group of formula wherein R 2 means a methyl or ethyl group; or A and B together form a group of formula A compound selected from the group consisting of 3-ethoxyoxalyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one, methyl 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylate, ethyl 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylate, 3-hydroxymethyl-9-methyl-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid lactone and 9-methyl-3-[(2-methyl-1H-imidazol-1-yl)methyl]-1,2,3,9-tetrahydro-4H-carbazol-4-one-3-glyoxylic acid. A novel process for the preparation of the compounds of the formula wherein A stands for a group of formula -CH 2 -R wherein R means a hydroxyl or 2-methyl-1H-imidazol-1-yl group; B represents hydrogen or a group of formula wherein R 1 means hydrogen or a methyl or ethyl group; or A and B together form a group of formula wherein R 2 means a methyl or ethyl group; or A and B together form a group of formula which comprises a) reacting the ketone of formula with a di(C 1-2 alkyl)oxalate in the presence of a basic agent in order to obtain a novel compound of the formula (Ia), wherein R 2 stands for a methyl or ethyl group; b) reacting a compound of the formula (Ia), wherein R 2 is as defined in step a) above, with formaldehyde in the presence of a basic catalyst in a non-acidic protic solvent, in order to obtain a novel compound of the formula (Ib) wherein R 1 is a methyl or ethyl group; or reacting a compound of the formula (Ia), wherein R 2 is as defined in step a) above, with formaldehyde in the presence of a basic catalyst in an aprotic solvent in order to obtain the novel compound of the formula (Ic) c) reacting a compound of the formula (Ib), wherein R 1 is as defined in step b) above, or the compound of the formula (Ic) with 2-methylimidazole of the formula (IV) in order to obtain the novel compound of the formula (Id); d) reacting the compound of the formula (Id) with a base, preferably an alkali metal carbonate or hydroxide, in order to obtain ondansetron of the formula (II), and, if desired, converting ondansetron of the formula (II) into its pharmaceutically acceptable acid addition salt. A process as claimed in claim 3, variant b), which comprises carrying out the reaction of. the compound of the formula (Ia), wherein R 2 is as defined above, with formaldehyde by using 1 to 2 moles of formaldehyde in the presence of not more than 0.2 mole of a basic catalyst. A process as claimed in claim 3, variant b) for the preparation of the compounds of the formula (Ib), wherein R 1 is as defined above, which comprises carrying out the reaction with formaldehyde in a C 1-2 alkanol. A process as claimed in claim 3, variant b) for the preparation of the compound of the formula (Ic), which comprises carrying out the reaction with formaldehyde in a dipolar aprotic solvent. A process as claimed in claim 3, variant d) which comprises splitting off the oxalyl group by alcoholysis of the C-C bond in the presence of a C 1-4 alkanol and binding the group removed by salt formation with a base stronger than 2-methylimidazole. A process as claimed in claim 3, variant c) which comprises using 2-methylimidazole in an amount of 1.0 to 3.0 moles, calculated for the compound of the formula (Ib), wherein R 1 means methyl or ethyl group, or for the compound for formula (Ic). A process as claimed in any of claim 3, variant c) or claim 7 or claim 8, which comprises using an aprotic solvent.

Inventors: BOD PETER DR, CSEHI ATTILA, FEKECS EVA, HARSANYI KALMAN DR, HEGEDUS BELA DR, HORVATH NEE SZIKI ERIKA, MERSICH NEE DONAT EVA, SZABO NEE KOMLOSI GYOERGYI, TRISCHLER FERENC DR, BOD, PETER, DR., CSEHI, ATTILA, FEKECS, EVA, HARSANYI, KALMAN, DR., HEGEDUS, BELA, DR., HORVATH NEE SZIKI, ERIKA, MERSICH NEE DONAT, EVA, SZABO NEE KOMLOSI, GYOERGYI, TRISCHLER, FERENC, DR., Bod, Péter, Dr., Harsányi, Kálmán, Dr., Hegedus, Béla, Dr., Horváth née Sziki, Erika, Mersich née Donát, Eva, Szabo née Komlosi, Györgyi
Applicants: RICHTER GEDEON VEGYESZET, RICHTER GEDEON VEGYESZETI GYAR R.T.
Application year: 1993
Main classification: C07D403
IPC codes: A61K31, A61P1, C07D209, C07D403, C07D491