POLYAZAMACROCYCLIC COMPOUNDS FOR COMPLEXATION OF METAL IONS

Abstract: The present invention relates to a new polyazamacrocyclic compound or a salt thereof and its uses as a tissue specific chelator. The compound has formula (I), where x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4; R is (CH2)ZP(=O)OR1OR2; R1 is H or CH3; R2 is CnH1+2n; n is 4 to 6; z is 1 to 3. In one important embodiment, this compound may be complexed with a metal to be a plyazamacrocyclic compound-metal complex having formula (II), where r is 2 or 3; and M is a metal ion, including a lanthanide, a heavy metal, or a radionuclide metal.

Description: DESCRIPTION POLYAZAMACROCYCLIC COMPOUNDS FOR COMPLEXATION OF METAL IONS BACKGROUND OF THE INVENTION Field of the Invention The present invention relates to compositions and methods for enhancing contrast in imaging internal structures and functions of living subjects. Imaging Modalities Imaging of internal structures and functions of living subjects may be accomplished by applying electromagnetic radiation from external sources (as in conventional x-rays and computerized axial tomography) or internal sources (as in PET or positron emission tomography and radionuclide scans) . Use of ionizing radiation is avoided in imaging with nuclear magnetic resonance (NMR) and untrasonography, making these methods advantageous for many applications. Whatever the imaging modality, consideration is given to means of increasing image contrast through localization of contrast agents in the region to be imaged. Such agents are frequently metals which emit, absorb, or scatter energy or, as in the case with NMR agents, increase the image signal strength locally. For best effect, agents must be localized. This may be accomplished, for example, by direct injection of contrast agent (as in myelograms or retrograde urethrograms) , through metabolic uptake of an agent (as in PET) , and by conjugation of contrast agents with monoclonal antibodies which tend to accumulate in certain tissues. The latter πrocess in oarticular has been used in NMR image enhancement with chelated metal ions. Though well known, the process has several shortcomings: l - preparation of the antibody is complex; 2 - diminished im unoreactivity of the antibody occurs following conjugation; 3 - there is limited uptake of the conjugate by the target tissue; and 4 - there may be unfavorable interactions between the chelated ion and the antibody. Because of the advantages of NMR imaging (good resolution and avoidance of ionizing radiation) , an NMR contrast agent capable of greater localization would be clinically important. Such an agent would offer significant advantages over contrast agents of the prior art. NMR Contrast Agents The quality of the images obtained from an NMR scan is based on two properties: the proton densities of the various tissues and differences in proton relaxation rates. The proton density of tissues cannot be readily altered. Proton relaxation rates can be adjusted by adding a paramagnetic relaxation agent, more commonly known as a "contrast agent." Contrast agents enhance the contrast in NMR images between magnetically similar but histologically dissimilar tissues. Gadolinium, which has strong paramagnetic properties because of its seven unpaired electrons, has been tested as a contrast agent. It has a large magnetic moment which efficiently relaxes magnetic nuclei and increases tissue contrast in the region of the gadolinium. One drawback of gadolinium as a contrast agent is its toxicity to animals, although a possible remedy for this problem is incorporation of gadolinium in a compound that would pass through the body and be excreted without releasing toxic gadolinium ions. Unfortunately, the rare earth elements (including gadolinium) do not form stable covalent bonds with organic molecules, so such molecules can decompose in vivo and release the toxic ions. Thus, there is a need for effective contrast agents which avoid the toxicity problems inherent in using gadolinium or another metal ion. Further, it is desirable that a contrast agent control or influence the distribution of chelated ions in the body. A even more desirable approach to the site-specific delivery of metal ions would be through use of stable chelates having inherent affinity for various tissue types. Inherent tissue affinity built into the organic chelating agent through modifications in both ionic charge and degree of lipophilic character would offer substantial advantages over currently available agents. SUMMARY OF THE INVENTION The present invention relates to a series of new phosphorous-containing triaza- and tetraazamacrocyclic chelators which have inherent affinity for certain tissues. Following intravascular injection, chelates comprising these compositions preferentially accumulate in certain tissues, depending on the time after injection. In particular, 1,4,7,10 tεtraazacyclododecane-1,4,7, lO- etra(methylenephosphonate monobutyl ester) has a high affinity for liver tissue and the gastrointestinal tract (in that order) . Chelates comprising this agent are thus suitable for liver imaging oecausε of the lipophilic character imparted by the ester functionality. Such agents are not metabolized, and eventually pass out of the body via the urine or feces. While the monobutyl ester above appears well adapted for liver imaging, analogous alkyl esters have also been considered. Monopentyl esters are nearly as good for liver imaging, but monooctyl esters have the disadvantage of very low aqueous solubility. Monopropyl esters, on the other hand, may be used for liver imaging but are less efficient because a substantial portion of the agent is rapidly lost to the kidneys; onoisopropyl esters would behave similarly. Hence, the most preferred embodiment is that described above with monobutyl esters. For use with NMR, compositions of the present invention must be chelated with a metallic element. While the element is preferably of the rare-earth series (preferably gadolinium) , those skilled in the art will recognize that other metallic ions might also be useful for imaging. For example, other metal chelates (e.g., chelates of radionuclides or heavy metals) may be used for imaging by scintigraphy, x-radiation, and analogous imaging methods where changes in local tissue parameters can increase image contrast. Depending on the metal ion preferred for a particular contrast agent application, either triaza- or tetraaza- compounds of the present invention may be selected as chelators. Chelators of the present invention have the formula r {(CH 2 ) χ NR} where x is 2 , 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or ; R is (CH 2 ) Z P(=0)OR 1 OR : ; R 1 is H or CH 3 ; R 2 is butyl, pentyl or hexyl; and z is 1 to 3. In one important embodiment, this compound may be complexed with a metal to be a polyazamacrocyclic compound-metal complex having the formula Γ {(CH 2 ) χ NR} y -, M +r where x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4; R is (CH 2 ) z P(=0)OR 1 OR 2 ; R 1 is H or CH 3 ; R 2 is butyl, pentyl or hexyl; z is 1 to 3; r is 2 or 3; and M is a metal ion. _ The y designation characterizes the compound as triazamacrocyclic or tetraazamacrocyclic. The x is preferably 2, although 3 is feasible under many circumstances. Combinations of p 2(ε) and q 3(ε) for x are of course readily produced but the total of p + q must be y for the number of units in the polyaza macrocycle. H or CH 3 for R 1 are believed equivalent in use. In a preferred embodiment of either the compound or its metal complex y is 3, p is 1 and q is 2 or p is 2 and q is l. In another preferred embodiment of the compound or its metal complex, y is 4, p is 1 and q is 3, p is 2 and q is 2 or p is 3 and q is 1 and z is most preferably 1. n is preferably 2. In a more preferred embodiment x is 2 , y is 4 , z is I, R 1 is H and R 2 is butyl. In another preferred embodiment X is 2 , y is 3 , z is l, R 1 is H and R 2 is butyl. The M +r is preferably a paramagnetic lanthanide, although other divalent or trivalent metal ions, including radionuclides and heavy metals, may also be so complexed. In one important application, the present invention involves a method for enhancing a magnetic resonance image of a subject. This method comprises administering to the subject a polyazamacrocyclic compound-metal complex having the formula {(CH 2 ) χ NR} ιM τr ; where x is 2 , 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or ; R is (CH 2 ) z P(=0)OR ; OR 2 ; R 1 is H or CH 3 ; R- is butyl, pentyl or hexyl; z is 1 to 3 ; r is 3 ; and M is gadolinium. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 schematically illustrates the structure of E (where R is CH 2 CH 3 and R 1 is H) . Figure 2 scnematically illustrates the structure of DOTEP, Figure 3 schematically i llustrates the structure of DOTPMB . DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS EXAMPLE 1 Triaza acrocyclic Compounds NOTPME synthesis Materials 1,4, 7-triazacyclononane, parafor aldehyde, diethylphosphite, and activated carbon Darco G-60 were purchased from Aldrich Chemical Company. MgS0 4 was from Mallickrodt, sodium hydroxide, and benzene from J. T. Baker, and diethylether from Fisher Scientific. All chemicals were of highest purity and were used without further purification. Solutions of ZnCl 2 , GdCl-,, MgCl 2 and Ca C1-. were standardized complexo etrically. Synthesis of NOTPME 1,4,7-Triazacyclononane (1.91 g, 14.71 mmol) and diethylphosphite (7.018 g, 16.94 mmol, 15% excess) were dissolved in 125 ml of benzene and heated to reflux. Anhydrous paraformaldehyde (1.727 g, 30% excess) was added in small portions to the above refluxing mixture while the benzene-water azeotropic mixture was removed by distillation. After the addition of paraformaldehyde was complete, the entire solution was boiled for 30 minutes and then evaporated to obtain a yellow viscous oil. The oil was dissolved in 150 ml anhydrous diethylether and dried with anhydrous MgS0 overnight. MgS0 4 , along with a white precipitate which formed, were filtered off and discarded. The filtrate was decolorized with activated carbon and filtered. The filtrate was evaporated in vacuum to obtain a viscous oil of 1,4,7- trιazacyclononane-N,N' ,N' '-tris(methylenephosphonate diethylester) (NOTPDE) . Pure NOTPDE was obtained in 96% yield (9.21 g, 14.17 mmol) and was used for the synthesis of NOTPME (structure shown in Figure l) without further purification. [ H NMR data of NOTPDE in CDC1 3 (TMS at 5 zero) are as follows: δ (ppm) : 1.33 (t, 18H, -CH 3 ) , 2.97 (s, 12H, N-CH 2 ) , 3.00 (d, 6H, P-CH 2 ) , 4.13 (p, 12H, 0- CH 2 ) . 9.20 g of NOTPDE (14.15 mmol) was mixed with 2.50 g 0 cf NaOH in 9 ml H-,0) and after 2 hours the entire reaction mixture was boiled until a clear solution was obtained (approximately 5 minutes) . The solution was cooled to room temperature and was allowed to stand overnight. The crystals formed were filtered off from the viscous mother 5 liquor using a pressure filter funnel with a coarse porosity grade filter disc. The crystals were washed once with cold absolute ethanol, three times with absolute ethanol-diethylether (1:1) mixture and finally with diethyl ether. The crystals of Na 3 NOTPME were dried 0 in dry nitrogen stream at 25°C for 2 hours. Traces of H-,0 and ethanol were removed upon vacuum drying (10 mm Hg) NOTPME for 5 hours at 50 "C. Pure NOTPME thus obtained were white crystals, very hygroscopic, readily soluble in H-,0, and fairly soluble in chloroform. The yield of pure 25 NOTPME was 40.8% (3.24 g, 5.77 mmol) . ] H NMR (D 2 0, HDO peak set as reference at 4.90 ppm) , o (ppm) : 1.23 (t, 9H, -CH 3 ) , 2.54 (s, broad, 6H, P-CH 2 ) , 2.79 (s, broad, 12 H, N-CH 2 ) , 3.91 (p, 6H, 0-CH 2 ) . 30 EXAMPLE 2 Tetraazamacrocyclic compounds DOTEP Synthesis DOTEP, shown in Figure 2, was prepared as follows. ΞΞ 2 ml of dichloroethylphosphine was slowly mixed with ice to form the corresponding ethylphosphinic acid. After warming to room temperature, 390 mg of 1,4,7,10- tetraazacyclododecane tetrahydrochloride (cyclen.4HC1) (Parrish Chem. Co. , Ogden, Utah) was added and the mixture heated to boiling under a nitrogen atmosphere. A solution containing 157 mg of paraformaldehyde dissolved in 10 ml of 6M HCl was added at a rate of 0.5 ml/hr, while the mixture continued to reflux. The final mixture was refluxed an additional 4 hours then cooled to room temperature. This solution was concentrated under vacuum to a viscous oil, redissolved into 6 ml of water and loaded onto a DOWEX 50Wx4 (hydrogen form) cation exchange column (7.5 ml bed volume). The column was washed to neutrality with water and the product eluted with 60 ml of 0.66 M HCl. The fractions containing DOTEP were combined, evaporated, redissolved in absolute ethanol and evaporated to a white solid. This solid was dispersed into anhydrous ether, filtered off, pre-dried under nitrogen and dried under vacuum at 60-70'C to yield a white, very hygroscopic solid (360 mg, 44% yield) . This solid was stored in sealed ampoules. Elemental analysis and potentiometry shows the solid to be DOTEP.2HC1 EXAMPLE 3 Tetraazamacrocyclic compounds DOTP Dibutyl Ester Synthesis Tetraaza-12-crown-4-4HC1 (1 g, 3.14X10 "3 mol) was dissolved in water and the pH adjusted to 9.0 using 1M NaOH. The solvent was evaporated and the residue dried under vacuum for 1 hour. Formaldehyde (6.6 mL of 37% solution, 7.15 g, 0.24 mol) was added and the solution stirred for 30 minutes at room temperature. Dibutyl phosphite (5.10 mL of 96% purity, 0.U25 mol) was then added and the reaction mixture stirred for 15 hours at room temperature (dipentyl and dihexyl phosphite are so used to produce dipentyl and dihexyl esters respectively) . The resulting mixture consisted of two layers. The bottom layer was mostly excess formaldehyde, as indicated by I3 C NMR. The upper layer contained the product and excess phosphite. This layer was separated, concentrated and dried under vacuum for l hour. The resulting syrup was loaded onto a silica-gel column (2.5 x 11 cm) . The excess phosphite was washed away with methylene chloride (250 mL) . The product was eluted with 5% methanol in methylene chloride. 20 mL fractions were collected and monitored by TLC. The fractions containing the product were combined, concentrated, and dried under vacuum, . A pale yellow oil was obtained in 75% yield (2.34 g) . ! H NMR (CDC1 3 ) : 0.87 (t, J=7.3 , 6H) , 1.33 (m, J=7.3, 4H) , 1.60 (p, 3=1 . 2 , 4H) , 3.18 (br s, 4H) , 3.39 (d, J=8.5, 2H) , 4.03 (m, J=7.3 , 6.1, 4H) . 13 C NMR (CDC1 3 ) : 11.3 (s) , 16.5 (s) , 30.3 (d, J=5.9) , 47.3 (d, J=148) , 50.0 (br s) , 63.8 (d, J=7.3). EXAMPLE 4 Tetraazamacrocyclic compounds DOTP Monobutyl Ester (DOTPMB) Synthesis The dibutyl ester was suspended in 1M KOH (20 mL) . The mixture was stirred at 85°C for 17 hours and then at 106°C for 9 hours. The solvent was evaporated and the sample dried under vacuum for 1 hour. Methylene chloride (40 mL) was then added and the remaining solid KOH crushed as much as possible. The solvent was again evaporated and this procedure repeated another two times. The solvent was evaporated and the residue dissolved in methanol (60 mL) . The mixture was filtered and then concentrated to a syrup under vacuum. Methylene chloride (80 mL) was added and the mixture filtered. The solvent was evaporated and the residue dried under vacuum to yield a white solid in 71% yield. 13 C NMR (D-.0; ref. dioxane at 67.0 ppm) : 13.5, 18.9, 32.9 (d, J=5.9) , 50.7 (d, J=140.6) , 51.5 (br s ) , 64.6 (d, J=5.9) . EXAMPLE 5 Biodistribution of Gd-DOTPMB Comolexation and Biodistribution A complex of DOTPMB (Figure 3) and Gd complex (0.012M based on metal, 2:1 ligand/metal ratio) was prepared and spiked with tracer quantities of Gd-159. Complexation was determined to be greater than 99% by standard analytical methods described in earlier reports. Two Sprague-Dawley rats were then injected with the complex at a 0.05 mmol/kg level. The animals were sacrificed after 30 minutes and dissected for biodistribution data (Tables I and II) ; actual counts obtained from various tissues are shown in Table II. At the end of this time period, an average of 58% of the injected dose was found in small intestine (see entry for SM INTES in Table I) . A similar experiment performed with a third rat yielded 52% in small intestine (Tables III and IV) ; actual counts obtained from various tissues are shown in Table IV. The bulk of the remaining activity in each case was eliminated via the renal system (Tables II and IV) . In order for localization to occur in the small intestine, the complex must first pass through the liver. Thus, since liver activity at the 30 minute time point (1%) was minimal (see e.g., Table I), the peak of liver localization passed within the prior 30 minutes. This is evident in an example of biodistribution 15 minutes after administration of chelated tracer, which is documented in Tables VIII and IX. Although by 15 minutes the peak of liver localization had passed for mouse 1, with 4% in the liver and 88% in the small intestine, mouse 2 still had a significant liver concentration (66%) at the 15 minute point. These animal models suggest that imaging within 15 minutes after administration of chelated tracer will be necessary for best definition of the liver. The following test description supports that conclusion. Higher doses would, of course, lengthen the time of liver localization at concentrations sufficient to substantially enhance liver imaging. Gamma Imaging of DOTPMB A Sm(153) -DOTPMB complex was prepared as described above for gamma imaging of a Sprague-Dawley rat. Images were acquired at one minute intervals over a 16-minute period. The image sequence revealed concentration of the chelate in liver within one minute following injection. The complex is then rapidly transported from the liver to the stomach and small intestine. Tables V, VI and VII contain data taken at 1 hour, 24 hours and 72 hours after injection, showing movement of the agent from stomach and intestine to feces. TABLE I TIME: 30 Minute Biodistribution DATE LIGAND METAL COMMENTS 8/6/91 DOTPMB-K, Gd-159, 99% Complexation 4:1, LIG:MET Molar Ratio, (Metal 3X10-4M) 0 5 0 5 0 5 C TABLE II (see legend for Table I) BKG AVG = 434 TABLE III TIME: 30 Minute Biodistribution DATE LIGAND METAL COMMENTS 8/6/91 DOTPMB-K, Gd-159 , 99% Complexation 4:1, LIG:MET Molar Ratio, (Metal 3X10-4M) 10 15 20 d m. 30 35 TABLE IV (see legend for Table III) BKG AVG 421 TABLE V One Hour Biodistribution, Imaged Rat TIME: One Hour biodistribution DATE LIGAND METAL COMMENTS 8/8/91 DOTPMB-K, D. Sm=153 99% Complex 4:1, LIG:MET Molar Ratio (Metal = 3X10-4M) TABLE VI 24 Hour Biodistribution, Imaged Rat TIME: 24 Hour biodistribution DATE LIGAND METAL COMMENTS 8/8/91 DOTPMB-K Sm=153 99% Complex 4:1, LIG:MET Molar Ratio (Metal = 3X10-4M) TABLE VII Rat Injected: 8/8/91 Time: 72 hour biodistribution Date: Ligand Metal Comments 8/12/91 DOTPMB-K Sm-153 99% Complex 4:1, LIG:MET MOLAR RATIO (Metal 3X10-4M) TABLE VIII File=BTY15STD Summary Standardized Data Mouse #2 (15 minute biodistribution) , 99% complex, 25.0 UL dose Ca added to complex at 1;1 molar, lig: Ca DATE LIGAND METAL COMMENTS 10/30/91 DOTPME-K Sm-15 #08-07-91 2:1, Lig:Met Molar Ratio, (Metal=3xlO-4M) , pH 7-=8 TABLE IX

Claims: Changes may be made in the construction, operation and arrangement of the various parts, elements, steps and procedures described herein without departing from the concept and scope of the invention as defined in the following claims. CLAIMS 1. A polyazamacrocyclic compound or a salt thereof, the compound having the formula {(CH,) χ NRh where x is 2 , 3 or a combination of p 2 (s) and q 3(s) where p + q = y; y is 3 or 4 ; R is (CH 2 ) Z P(=0)OR 1 OR : ; R 1 is H or CH 3 ; R 2 is C r H 1+2n ; z is 1 to 3 ; and n is 4 to 6. 2. A polyazamacrocyclic compound-metal complex having the formula r { ( CH 2 ) χ NR} v - K + T where x is 2 , 3 or a combination of p 2(ε) and q 3(s) where p + q = y; y is 3 or 4 ; R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H or CH 3 ; R- is Cπ n Hl 1+ +27nn<' * ~ . -" I r is 2 or 3 ; M is a metal ion; and n is 4 to 6. 3. The compound of claim 1 where y is 3. 4. The compound of claim 1 where y is 4. 5. The compound of claim 1 where y is 3 and x is 2 , 6. The compound of claim 1 where y is 4 and x is 2, 7. The complex of claim 2 where y is 3. 8. The complex of claim 2 where y is 4. S. The complex of claim 2 where y is 3 and x is 2. 10. The complex of claim 2 where y is 4 and x is 2. 11. The compound of claim 1 where y is 3 , p is 1 and q is 2 or p is 2 and q is 1. 12. The complex of claim 2 where y is 3 , p is 1 and q is 2 or p is 2 and q is 1. 13. The compound of claim 1 where y is 4, p is 1 and q is 3, p is 2 and q is 2 or p is 3 and q is 1. 14. The complex of claim 2 where y is 4, p is l and q is , p is 2 and q is 2 or p is 3 and q is 1. 15. The compound of claim 1 where z is 1, 16. The complex of claim 2 where z is 1. 17. The compound of claim 1 where R 2 is C 4 H 9 , IS. The complex of claim 2 where R 2 is C 4 H 9 . 19. The complex of claim 2 where M is a lanthanide element. 20. The complex of claim 2 where M +r is Gd +3 , 21. A compound or salt thereof, the compound having the formula: 0 O-R N P ' OR' R' O N N - o OR 1 R where R is C n H 1+2n ; n is 4 to 6 ; and R' is H. 22. A compound or salt thereof, the compound having the formula: R 2 0 R 1 R 2 0 N Ni ¬ .N N- O OR- R 1 OR 2 where R 1 is OC n H 1+2n ; n is 4 to 6; and R 2 is H. 23. The compound of claim 21 or 22 where n is 4 24. A method for increasing contrast of nuclear magnetic resonance images of a patient, the method comprising administering to a patient needing nuclear magnetic resonance imaging a polyazamacrocyclic compound or a salt thereof, the compound having the formula {(CH 2 ) χ NR} v M " where x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4; R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H or CH 3 ; n is 4 to 6; z is 1 to 3 ; r is 2 or 3 ; and M is a metal ion; and obtaining a magnetic resonance image of the patient. 25. The method of claim 24 wherein x is 2, y is 3, z is 1, and n is 4. 26. The method of claim 24 wherein x is 2 , y is 4 , z is 1, and n is 4. 27. The method of claim 24 wherein M is a lanthanide element. 28. The method of claim 24 wherein M is Gd +3 29. A method for enhancing an x-ray image of a subject, the method comprising administering to the subject a polyazamacrocyclic compound-metal complex having the formula where x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4; R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H or CH 3 ; -> R- is C π H 1+2n ; n is 4 to 6; z is 1 to 3 ; M is a heavy metal; and _- r is 2 or 3 ; and obtaining a magnetic resonance image of said subject. 1 0. A method for enhancing a scintigraphic image of a subject, the method comprising administering to the subject a polyazamacrocyclic compound-metal complex having the formula where x is 2 , 3 or a combination of p 2(s) and q 3 (s) where p + q = y; y is 3 or 4 ; R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H or CH 3 ; R 2 is CH, .-,--; is 1 :o 3 M is a radionuclide metal; and r is 2 or 3; and obtaining a scintigraphic image of said subject. 31. The method of claim 30 wherein M is samarium 153 32. The method of claim 29 or 30 wherein x is 2, y is 3, z is 1, and n is 4. 33. The method of claim 29 or 30 wherein x is 2 , y is A , z is 1, and n is 4. 34. A polyazamacrocyclic compound or a salt thereof, the compound having the formula {(CH 2 ) χ NR} y where x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4 R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H; R 2 is C 4 H 9 ; and z is 1 to 3. 35. A polyazamacrocyclic compound-metal complex having the formula where SU BSTI TUTESHEET x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4; R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H; R 2 is C 4 H 9 ; z is 1 to 3; r is 2 or 3; and M is a metal ion. 36. A method for increasing contrast of nuclear magnetic resonance images of a patient, the method comprising administering to a patient needing nuclear magnetic resonance imaging a polyazamacrocyclic compound or a salt thereof, the compound having the formula where x is 2, 3 or a combination of p 2(s) and q 3(s) where p + q = y; y is 3 or 4; R is (CH 2 ) Z P(=0)OR 1 OR 2 ; R 1 is H; R 2 is C 4 H 9 ; z is 1 to 3; r is 2 or 3; and M is a metal ion; and obtaining a magnetic resonance image of the patient. 37. The complex of claim 35 where M +r is Gd +3 . 38. The method of claim 36 where M +r is Gd +3 . S UBSTIT UTESHEET 39. A compound or salt thereof, the compound having the formula: OH SUBSTITUTE SHEET

Inventors: KIEFER GARRY E, SHERRY ALLAN DEAN, KIEFER, GARRY, E., SHERRY, ALLAN, DEAN
Applicants: DOW CHEMICAL CO, UNIV TEXAS, BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, THE DOW CHEMICAL COMPANY
Application year: 1993
Main classification: C07F9
IPC codes: A61K49, A61K51, C07D255, C07D257, C07F9