Use of aminoalcohol-N-acylderivatives for the manufacture of a medicament for the treatment of neurogenic endoneural oedema of the peripheral nerve

Abstract: Aminoalcohols N-acyl derivatives useful in the prevention and in the treatment of human and animal pathologies induced by mast cell degranulation following to a peripheral nerve noxa.

Description: FIELD OF THE INVENTION The present invention relates to the use of aminoalcohols N-acyl derivatives for preparing pharmaceutical compositions for the prevention or the therapeutic treatment of human and animal pathologies involving a neurogenic endoneural oedema induced by mast cells degranulation as a consequence of a peripheral nerve noxa. PRIOR ART DISCLOSURE It is known that noxae of different origin in the peripheral nervous system determine significant alterations both in the neural components and in the microenvironment of the injured nerve. From a strictly anatomical point of view the peripheral nerve is a complex structure of neural components, namely Schwann cells and axons, and non-neural components, that is connective matrixes, extracellular fluids and blood vessels. The extracellular fluids, together with the connective matrixes form the microenvironment, through which the exchanges of the exogenous nutritious substances and endogenous substances produced by the same neural cells (mainly the Schwann cells, because analogous exchanges between the axons and the blood is accomplished through the axoplasmatic flow) occur between the blood and these neural cells. These two components, e.g.the microenvironment and the neural portion, together form a nervous fasciculus which structurally results to be separated by the perineurium from the surrounding tissues, so that a tubular structure originates, being physically delimited by the perineural barrier functionally comparable with the hematoencephalic barrier. The purpose of this barrier is to regulate the fine homeostasis between the inside (endoneurium) and the outside (epineurium); the perineurium has therefore the function of a quali-quantitative homeostatic regulation of the microenvironment fluids with respect to the external ones. Another structurally and fundamentally important element for the homeostatic equilibrium maintenance of the microenvironment extracellular fluids consists in "vasa nervorum", the blood vessels through which the blood arrives at the nerves. The endoneural extracellular fluids are in fact in equilibrium with blood serum, which arrives through these vessels. Blood vessels are in turn innervated by free nerve endings, the so called "vasa nervorum". In other words they are intramural terminations innervating the vasa nervorum, having essentially a peptidergic origin. The main peptides contained in these terminationes are substance P, bradykinin, VIP and NPY. These terminations also contain conventional neurotransmitters such as serotonin and dopamine ( Appenzeller O. et al. , Brain Res. 1984, 304(2) ,383-386). Under many lesive conditions of the peripheral nerve, having independently traumatic, toxic or dysmetabolic causes, a hyperactivation situation is observed for these nervous endings which release high amounts of the aforementioned neuropeptides, locally active in the area determined in the epineural barriers. The etiopathogenetic meaning of this nervous hyperstimulation becomes clear, if one recognizes that a narrow microanatomic relationship exists between the nervous peptidergic endings and particular immunocomponent cells, like the mast cells (Heine, H. et al., Z. Mikroskop. Anat. Forsch. 1975, 89,934-937). In this regard it is important to remember that mast cells are widely present in the peripheral nerve both in the epineurium where they are located along the blood vessels, and in the perineurium where they are associated, too, with the capillary vessels. Mast cells are also present in the endoneurium, more in the distal portion of the nerve than in the proximal one (Olsson, Y. Int. Rev. Citol. 1968, 24,27-70). It is furthermore important to point out that the most recent research has demonstrated a dramatic increase of RNA messenger for the NGF (nervous growth factor), as well as the NGF protein synthesis occurring in neural and non neural cells (Schwann cells and fibroblast type cells) associated with the nerve involved in the lesion (Lindholm, D. et al Nature 1987, 330, 658-659); the above mentioned phenomena are regulated by the synthesis of a cytokine: interleukin-1, produced by the activated macrophages (Wehling, P. et al Neuro-orthopedics 1989,7, 55-59). This marked increase of NGF in the lesion site determines in its turn a considerable increase of the number of local mast cells number acting as "gate keepers", and therefore as amplifiers of the morphofunctional phenomena as a consequence of a lesive noxa.( Aloe.L., Levi Montalcini R., Brain Res. 1977, 133, 358-366; Aloe L et al. Olin. Exp. Rheumatol. 1992, 10, 203-204). The peptidergic nervous fiber-mast cell functional unit is thus involved in many paraphysiologic events such as pain, neurogenic inflammation and the local regulation of the blood flow (Foreman, J.C. Allergy 1987, 42, 1-11). It is in fact important to remember that the substance P release induced by nervous stimulation causes a mast cell hyperactivation condition able to determine degranulation of the preformed mast cells stores of mediators such as histamine, serotonin, tumor necrosis factor (TNF), involved in the induction, amplification of the inflammatory processes and formation of local oedema. Under many conditions of the peripheral nerve injury caused or associated with inflammatory processes alterations of the perineurium are observed, but above all very significant changes are noted both in the composition and in the amounts of endoneural fluids with impressive oedema conditions, which, although generally representing a consequence of the nervous stimulation induced by the injury and therefore not being its primary cause, actually determine a significant worsening of the pathologic situation. Following to the neurogenic oedema onset, significant alterations of the vascular permeability, both of the endoneural and of the perineural microvessels take place, thus compromising the protective function of the perineural barrier. The oedema can occur at the level of any nervous compartment; in other words following to a noxa a fluids accumulation may be observed at the perineural, epineural level or again at the endoneural one. Besides the anatomic localization, it is important to distinguish between the oedema characterized by an alteration of the vasal permeability to proteins with their consequent extravasation, and the oedema not having this protein leakage. Under many conditions of peripheral nerve injury, a consistent pattern of endoneural neurogenic oedema take place together with a remarkable protein extravasation as a consequence of the nervous hyperstimulation. During the neurogenic endoneural oedema formation, the first crucial event consists of the perineural barrier nerve / blood breaking , determining a precocious protein leakage, wherein, as above said, mast cells activation and the consequent release of the endogenous neurotransmitters play a key role. Mast cell degranulation, following peripheral nerve traumas with consequent mediators release, such as histamine, can increase the vasal permeability which in the first place is the main cause of the oedema formation ( Olsson Y. Int Rev. Citol. 1968,24,27; Nennesmo I. et al . Nuerosci, Lett.1986,69, 296). Actually the same mediators, successively in association with inflammation specific mediators, after causing a rapid increase of the endoneural pressure for the oedema formation, slow down the vascular permeability, by further increasing the endoneural pressure, thus determining secondary degenerative type injuries (Mellick, R.S. et al. , J.Neurol. Neurosurg. Psychiatry 1967, 30, 458. The neurogenic endoneural oedema has therefore important implications from a pathogenic point of view under many peripheral, somatic and autonomic neuropathological conditions. In fact, besides slowing down the endoneural microcirculation, therefore blocking the nutritious substances supplied by a mechanical type action to the neural components, which, as a consequence of that, lead to death, the oedema condition favours, in presence of a high protein concentration deriving from the protein leakage increase, a remarkable production of collagen and of a cultural medium, being suitable for the proliferation of fibroblasts, mast cells and Schwann cells, which are able to induce a fibrosis aiming to compromise the nerve functionality. Therefore, the oedema formation at the level of the microenvironment (matrix and extracellular fluids) is associated to other pathologically important alterations, as for example fibroblasts and mast cells proliferation, alterations which may assume a primary pathogenic role. It is finally important to point out that, following the neurogenic oedema formation, a vicious circle starts, where mast cells play a primary role in the induction (first phase of the oedema induced by mast cell degranulation) and in the worsening of the oedema state (second phase of the oedema associated with fibrosis and mast cells proliferation). In other words following to a peripheral nerve lesion an immediate first type cellular reaction occurs in the lesion site, identifiable as a degranulation process of the endoneural mast cells, followed by a release of mediators which results in an oedema formation. As a consequence of the oedema condition, a process of cellular proliferation starts, where an important component is again represented by mast cells. In this second phase also distal alterations, with respect to the primary site of lesion, known as Wallerian degeneration, appear at the level of the nerve. Also in the case of the Wallerian degeneration the increase in mast cells proliferation is considerably higher in the endoneural compartment than in the peri- and epineural one, occurring in the distal portion of the nerve. (Olsson Y. et al., Acta Neuropath. 1969, 13, 111). The oedema formation is in any case associated with an impressive alteration of the perineurium permeability at the lesion site (Olsson Y et al., Rev Adv, Neuropathol. 1979, 2,1). This modification starts very quickly during the first hour from the injury and lasts for some weeks, but it seems to involve only the proximal portion of the lesion and not the distal one and it does not seem to be connected with a protein leakage. Furthermore the perineurium oedema does not seem to be sensitive to the inflammation mediators. Therefore from a pathologic point of view the neurogenic endoneural oedema results to be of primary importance in the peripheral nerve pathologies, due to these above mentioned features. It appears evident from the above that from a therapeutical point of view it would be very important to have compounds able to control mast cell hyperactivation and degranulation as a consequence of the peripheral nerve noxae of various origin, determining the onset of the neurogenic endoneural oedema. The involvement of mast cell degranulation in the pathogenesis of rheumatic diseases, in particular of rheumatoid arthritis, and in the outset of the inflammatory process in experimental allergic neuritis, accompanied by endoneural oedema formation, is known in the state of the art (J.M. Mican et al., J.Allergy Clin. Immunol., vol.86, 4: 677-683, 1990; H.C. Powell et al., Laboratory Investigation, vol. 48, 3:332-338, 1983). Quite recently the Applicant has found compounds active in modulating mast cells degranulation processes, acting with a local antagonist autacoid type mechanism of action, and which can be advantageously used in the treatment of autoimmune pathologies, as described in the European Patent Application 92121862.4. It is in fact known that, in autoimmune diseases, pathologic autoaggressive phoenomena occur, revealing themselves through processes of localized tissue damage where specific immunocompetent cells, and among them mast cells, play a central aetiopathogenetic role. Mast cells are in fact a cellular population residing in tissues which, self activating in situ, proceed to predetermine the biologic sequence of the inflammation, releasing several neurotransmitters, often characterized by a considerable cytotoxicity, and are responsible for the localized tissue damage. Anyway it is known that mast cells activity is regulated in agonist manner by neuro and immuno-mediated activating systems, antagonized by inhibitory systems, revealing themselves through general circuits, as for example corticosteroid hormones. The Applicant has found that the compounds derived from the N-acylation of aminoalcohols such as mono and diethanolamine, can act on mast cells as antagonist local systems, through an autacoid type mechanism, and as a consequence of that, they can be advantageously used in the therapy of autoimmune pathologies. Also N-(2-hydroxyethyl)hexadecanamide, or N-palmitoylethanolamide (N-PEA), belongs to this class of compounds. The activity of this compound was casually discovered around the Fifties, in consequence of the identification of a generic cytoprotective activity of the lipidic excipient, in which this compound was present, of an antirheumatic drug. The pharmacologic profile of this compound was then studied in experimental patterns by following damages caused by different agents; the interesting ability in increasing the resistance of the animals to various bacterial toxins was deemed the most suitable for the successive pharmaceutical development of this compound. In fact it is on this base that a pharmaceutical product in the form of tablets was launched in Czechoslovakia, whose therapeutical indication was the prevention of infections of the respiratory tract. F.A. Kuehl Jr. et al. (J.Am.Chem.Soc., vol. 79, 19:5577-5578, 1957) described the generic anti-inflammatory activity of N-PEA and attributed said activity to the alcoholic moiety of the molecule (i.e. ethanolamine). F. Perlik et al. (Future Trends in Inflammation, Piccin Medical Books, pages 301-306, Verona 1974) investigated the effect of N-PEA on inflammatory processes, such as rheumatoid arthritis; neither a teaching concerning the activity of said compound on mast cells, not the involvement of mast cells in the above pathologies was reported. With respect to what is already known, the Applicant has in fact found that both the activity of N-PEA, and that of the compounds belonging to the wide class of amino alcohols N-acyl derivatives described in the European Patent Application above cited, is not only directed to a generic and moderate cytoprotective activity, as suggested by the prior art for N-PEA, but on the contrary said class exhibit a specific and important role in the inhibitory modulation of mast cell degranulation and therefore in inhibiting the autoaggressive effects of the autoimmune pathologies. This is the consequence of the inhibition of the uncontrolled release of preformed mast cell granules,containing numerous proinflammatory mediators and in particular preformed granules containing the Tumor Necrosis Factor, a highly cytotoxic cytokine involved in the autoaggressive autoimmune process (Toms R. et al., J.Neuroimmunology,1990,30, p.169-177; Kruger P.G. et al., Acta Neurol. Scand., 1990, 81, p.331-336). What is described in the above mentioned European patent application does not imply an involvement of these compounds on the neurogenic endoneural oedema under peripheral nerve damage conditions, since the endoneural compartment results to be effectively protected by the barrier formed by the perineurium, whose function was previously described. Therefore it does not result as an obvious consequence the ability of the same compounds in blocking endoneural mast cells degranulation, induced by a nervous hyperstimulation on lesive base of the peripheral nerve. In any case it is not evident on the basis of what is already known or previously discovered by the same Applicant that these compounds can act as local antagonist autacoids, also at the level of endoneural mast cells of the peripheral nerve. And in any case this action is more and more important if we consider that, following to a primary effect in inhibiting mast cells degranulation, these compounds are not only able to prevent the endoneural oedema formation, but also to prevent mast cells proliferation, which is the determining event of starting the oedema vicious circle being the responsible of the chronicity and the seriousness of the pathology. SUMMARY OF THE INVENTION The Applicant has in fact found that the degranulation and the consequent mast cells proliferation occurring following traumatic, toxic or dysmetabolic noxae of the peripheral nerve, can be effectively antagonized by administering aminoalcohols N-acyl derivatives, as these compounds are able to control the neurogenic endoneural oedema, associated with pathologic states of the peripheral nerve. The present invention therefore relates to the use of said compounds in the preparation of pharmaceutical compositions for the prevention or the treatment of pathologies of the peripheral nerve, having traumatic, toxic or dysmetabolic origin, characterized or associated with neurogenic endoneural oedema. DETAILED DESCRIPTION OF THE INVENTION The characteristics and advantages of the aminoalcohols N-acyl-derivatives of the present invention, acting as local autacoids and therefore utilizable in the treatment of pathologies of the peripheral nerve system, characterized by a neurogenic endoneural oedema, will be better understood in the light of the instant detailed description. The Applicant has surprisingly found that the activity of N-PEA is not limited to a generic cytoprotective activity, as suggested by the prior art, but that this compound, being able to modulate also mast cells degranulation in this specific and protected neurogenic endoneural compartment, plays a specific and important role in controlling the neurogenic and endoneural oedema in this compartment of the peripheral nerve. For this reason this compound is utilizable in the preparation of pharmaceutical compositions suitable to be administered in man and animal for the treatment of pathologies of the peripheral nerve as a consequence of noxae of different (e.g traumatic, toxic or dysmetabolic) origin. More generally, this pharmacological activity being related to mast cells degranulation modulation mechanism , which the whole class of the aminoalcohols N-acyl derivatives resulted active to, the same activity can be claimed not only for N-PEA, but for the entire class of the compounds according to the present invention. In order to define better the present invention, the compounds belonging to the above mentioned class of the N-acyl derivatives are characterized by the following formula: wherein R 2 is an alcoholic residue selected from a C 1 -C 20 linear or branched hydroxyalkyl, optionally substituted in the alkyl chain with at least one aryl group, and a hydroxyaryl, optionally substituted on the aromatic ring with at least one linear or branched alkyl radical of from 1 to 20 carbon atoms; wherein R 3 is H or is = R 2 ; and wherein is an acyl radical of a monocarboxylic acid. Preferred alcoholic residue R 3 and/or R 2 according to the present invention, for a merely illustrative but not limitative purpose, are those of monoethanolamine, diethanolamine, 2-hydroxypropylamine, di-(2-hydroxypropyl)-amine, which bring to the compounds having the following formulas: The N-acylderivatives of 2-hydroxy-propylamine and of di-(2-hydroxy-propyl)-amine may be an optical isomer or a raceme. As regards the acyl portion of the molecule of the N-acyl derivatives according to the present invention , namely the R 1 -CO group, it is preferably the acyl radical of a monocarboxylic acids. Among these, the monocarboxylic saturated or unsaturated acids, optionally substituted with a hydroxy or aminic group on the aliphatic chain, and the monocarboxylic acids containing aromatic, heteroaromatic and/or heterocyclic groups are to be considered. For illustrative purposes, in the group of the monocarboxylic aliphatic acids suitable to acylate the nitrogen of the above mentioned aminoalcohols are to be considered all the acids of biological importance as for example palmitic, oleic, stearic, lauric, myristic and their hydroxy and amino homologues. Among the acids containing aromatic, heteroaromatic and/ or heterocyclic groups the following can be used : salicylic, acetylsalicylic, sulfosalicylic, benzoic, trimethoxybenzoic, α-lipoic (= thioctic acid), retinoic, thenoic, phenylanthranilic, hydroxyphenylacetic and all the biologically acceptable acids. The following examples describe the subject of the present invention and the best way to carry out the invention, but in no way they limit it. Example 1- N-palmitoylethanolamide (N-PEA) synthesis Following Roe E.T. et al. indications (J. Am Chem. Soc., 1952, 74, 3442-3443), N-palmitoylethanolamide synthesis was accomplished by reacting under reflux ethanolamine with palmitic acid. Particularly 1 mole of palmitic acid was reacted in the presence of 1.5 moles of ethanolamine in ethyl ether for 5-6 hours under nitrogen atmosphere. The reaction product was then extracted from the reaction mixture and crystallized by using 95 % ethanol at a temperature of 0 °C. The melting point found for N-PEA was around 94-95 °C. The physical chemical characteristics of the N-PEA product synthesized according to the present example were the following : - physical state crystalline powder - raw formula C 18 H 37 NO 2 - molecular weight 299.48 - elemental analysis C72.19%; H12.45%; N 4.68%; O10.69% - solubility in organic solvent Hot MetOH, CHCl 3 , DMSO - solubility in water insoluble - melting point 94-95 °C - TLC in chloroform/methanol (9:1) Rf = 0.75 N-Palmitoyldiethanolamide and the other compounds of the invention were prepared by a similar process, as described in the above mentioned European Patent Application 92121862.4. i) Biological activity against mast cell degranulation of the peripheral nerve. In order to verify the pharmacological activity of N-acyl derivatives of aminoalcohols, and in particular of N-PEA, under conditions of traumatic noxa due to crushing of the sciatic nerve, and therefore able to induce mast cells degranulation, the following "in vivo" biologic test was carried out. Materials and Methods Male Spraugue Dawley Rats weighing about 200 -250 g were divided into two groups and subjected for 15 days to two different treatments: the first group, being the control group, was intraperitoneally treated with a solution of liposomes of soy lecithin, whereas the second group was intraperitoneally treated with 20 mg/kg N-PEA vehiculated on soy lecithin liposomes. The choice of this specific vehicle depended on the poor solubility of N-PEA in aqueous vehicles. At the 16 th day the animals were subjected to a bland general anesthesia with ethyl ether, thereby proceeding to the operation of the nerve lesion as hereinbelow described. The left sciatic nerve was exposed just in the spot of its entrance to the haunch, by operating a dissection of the muscular bands. The thus exposed sciatic nerve was then repeatedly compressed as closest as possible to its origin, by using a crushing forceps. The wound was temporarily sewn up and the animals were left to recover. Three hours after the sciatic nerve compression lesion the animals were sacrificed in order to take the sciatic nerve samples and to fix it for the histological analysis with a mixture of acetic acid and formalin. The tissues were then cut into fine sections, colored with a masts cell specific dye, 5% toluidine blue in a citrate buffer at pH 4.5, as described by Enerback L., et al. (Zeit. Zellforsch. 1965, 66, 596). The thus prepared tissues were then examined at the optical microscope in order to count mast cells. Results With the purpose of evaluating the pharmacologic effects of NPEA, a comparative analysis of the number of degranulated mast cells of the two rats groups was conducted. For each animal, a total nummber of 100 cells in sections in the proximity of the sciatic nerve lesion were examined. The results are hereinbelow reported in tables 1 and 2: Groups Number of degranulated cells on a total of 100 examined cells CONTROL: rat No.1 43 rat No.2 74 rat No.3 65 rat No.4 68 rat No.5 57 average 61.4 Groups Number of degranulated cells on a total of 100 examined cells N-PEA 20 mg/kg rat No.1 35 rat No.2 44 rat No.3 37 rat No.4 25 rat No.5 42 average 36.6 As it results from the foregoing, N-PEA is also able to modulate mast cell degranulation processes activated by a traumatic compressive type noxa. In this way it is able to exert a pharmacological effect on neurogenic oedema induced by mast cells activation process. The pharmacologic activity of N-PEA, common to the entire class of the aminoalcohols N-acylderivatives whose N-PEA represents the first example, plays a significant role in all the pathologic states of the peripheral nervous system wherein, in consequence of a peripheral nerve noxa both of traumatic type and of toxic or dysmetabolic origin, a sufferance of the nerve occurs, associated with a neurogenic endoneural oedema. In practice this is a category of pathologies universally known as peripheral neuropathies, according to a definition given by the World Health Organization (WHO) in WHO-Study group held in Geneva in October 1979, and contained in a Specific Technical Report (Peripheral Neuropathies-Technical Report Series No. 654-WHO 1980). Although the causal agents of peripheral neuropathies are various (varying from chemical agents such as solvents, pesticides, viral or bacterial agents [herpes zoster, HIV, diphtheric toxin], to metabolic causes such as diabetes and uremia and to traumatic-compressive causes such as those occurring in intravertebral disc protrusion), as to their classification the World Health Organization divides peripheral neuropathies into three main categories : neuropathies having traumatic, toxic and dysmetabolic origin. Generally the compounds according to the present invention are parenterally (intravenously, intramuscularly and subcutaneously) or orally administered. In any case under anatomically localized neuropathic conditions, such as localized traumatic lesions, topic (dermic, intra- and trans-dermic) administrations are not to be excluded. The therapeutically effective doses vary depending on the administration route and on the application modalities, as well on the pathology severity; other factors are also to be considered connected with the patients' age, weight and health general conditions. A therapeutically acceptable range may be any way comprised between 0.1 and 50 mg/kg and preferably between 1 and 20 mg/kg. Relevant undesired effects of the compounds according to the present invention being unknown, further to the therapeutic dose also a therapeutic regimen has to be established on the base of medical criteria, taking into account the acuteness or chronicity characteristics of the pathology. Typically a therapeutic regimen may be of from 1 to 2 daily administrations of the drug for 3-4 weeks, even in therapeutic cycles repeated for at least three months. Anyway, under particular acute neuropathic states, short duration regimen are not to be excluded, being characterized by 3-4 daily administrations for 1 or 2 weeks. Furthermore, as these pathologies are characterized by new acute phases, the aminoalcohol N-acylderivatives according to the present invention can be advantageously used for a preventive action, as well as for a therapeutic one. As preventive agents in chronic pathologies characterized by recurrent acute episodes, these compounds can be administered as dietetic integrating components, and the daily dosage foreseen for this specific use of the compounds according to the present invention preferably range from 0.1 to 1 mg/kg, both for human beings and for animals. The compounds according to the present invention can be formulated in pharmaceutical compositions comprising all those substances suitable for the above mentiones administrations and the excipients can be those pharmaceutically and therapeutically suitable for the same applications as well those of a more recent conception able to improve the delivery of these active principles to the site of action. The preferred formulations for the topical (dermic, intra- and trans-dermic) administration are buffered solutions, gels, medicated plasters, whereas for the systemic oral administration all the formulations are suitable in the form of dry powders such as granulates, tablets, dragees, soft or hard jelly perles, containing the compounds dissolved or dispersed in lipidic excipients; in the liquid form as suspensions, aqueous solutions or emulsions wherein these compounds are present even in the form of liposomes, optionally mixed with other lipids; in semisolid form obtained with polysaccharides aqueous suspensions, solutions or emulsions containing the compounds according to the present invention in the form of liposomes optionally mixed with other biologically acceptable lipids. The dietetic integrating components are preferably in the form of dragees, tablets or oily perles. For the rectal administration, dispersions of the compounds according to the present invention can be utilized in the form of dispersions in an acceptable phospholipidic excipient. As to the parenteral administration, the preferred formulations are aqueous buffered solutions or emulsions, containing the compounds of the present invention optionally in the form of simple or mixed liposomes with other biologically acceptable lipids for the parenteral use, or oily solutions; these parenteral formulations can also consist of a lyophilized product readily dispersable in the solvent at the moment of the administration. With the aim to rendering evident the possible industrial use of the compounds according to the present invention we report hereinbelow some examples of preferred pharmaceutical compositions. These composition are only provided for illustrative, but they are in no way to be considered as limitative of the present invention. Example 1 Tablets : Every tablet contains : N-palmitoylethanolamide 300 mg lactose 35 mg maize starch 90 mg polyethylene glycol 5 mg carboxymethyl cellulose 15 mg magnesium stearate 5 mg Yellow Fe oxyde (E172) 0.2 mg Example 2 Soft Jelly Perles : Every perle contains : N-palmitoylethanolamide 100 mg soy lecithin 40 mg peanut oil 100 mg jelly 52 mg glycerol 16 mg erythrosin (E127) 0.1 mg Example 3 Chewable Jelly Drops : Every drops contains : Mixed liposomes obtained by sonication and containing : - N-palmitoylethanolamide 200 mg - soy lecithin 100 mg p-hydroxybenzoate 2 mg purified carrageenin 40 mg demineralized water 1.66 g Example 4 Syrup : 100 g of syrup contain : Mixed liposomes obtained by sonications and containing : - N-palmitoylethanolamide 2 g - soy lecithin 1 g methyl p-hydroxybenzoate 0.1 g glycerin 1.7 g vanilla tincture 0.15 g orange tincture 0.6 g saccharose 19 g demineralized water q.s. to 100.0 g Example 5 Suppository : Every suppository contains : N-palmitoylethanolamide 30 g soy lecithin 50 mg triglycerides of fatty acids q.s. to 1.5 g Example 6 Vials : Every vial contains : N-palmitoylethanolamide 30 mg soy lecithin 100 mg phosphate buffer pH 7.6 2 ml Example 7 Vials : Every vial contains : n-palmitoylethanolamide under liposomes form obtained by sonications 150 mg mannitol 15 mg bidistilled apyrogen buffered water q.s. to 1.5 ml Example 8 Lyophilized Tiny Bottle : Every lyophilized tiny bottle contains : N-palmitoylethanolamide 500 mg mannitol 30 mg glycocoll 100 mg Every vial contains : bidistilled apyrogen buffered water 2.0 ml Example 9 Dermatologic Cream : 100 g of cream contain : N-palmitoylethanolamide 50 mg sorbitan monostearate 500 mg polyoxyethylen sorbitate 4.5 g ethanol 3 g stearic acid 3 g paraffin oil 10 g 70 % sorbitol 6 g p-oxybenzoic acid methyl ester 0.2 g p-oxybenzoic acid propyl ester 0.05 g water q.s. to 100 g Example 10 dietetic integrating component in jelly perles Every perle contains: N-palmitoylethanolamide 30 mg egg lecithin 90 mg maize oil 240 mg The pharmaceutical compositions containing the compounds according to the present invention may find a valid therapeutic and preventive application in all the pathologies characterized by an injury of the peripheral nerve associated with a neurogenic endoneural oedema substained by mast cells hyperactivation. In particular the compounds of the present invention can be advantageously administered in the pathologies of the peripheral nervous systems, normally those defined as peripheral somatic and autonomic neuropathies having traumatic, toxic or dysmetabolic origin.

Claims: Claims for the following Contracting States : AT, BE, CH, LI, DE, DK, FR, GB, GR, IE, IT, LU, MC, NL, PT, SE Use of one or more aminoalcohols N-acylderivatives, having the formula: wherein R 2 is an alcoholic residue selected from a C 1 -C 20 linear or branched hydroxyalkyl, optionally substituted in the alkyl chain with at least one aryl group, and a hydroxyaryl, optionally substituted on the aromatic ring with at least one linear or branched alkyl radical of from 1 to 20 carbon atoms; wherein R 3 is H or is = R 2 ; and wherein is an acyl radical of a monocarboxylic acid; as the active ingredient for the preparation of pharmaceutical compositions for the prevention or the treatment of human and animal pathologies involving a neurogenic endoneural oedema induced by mast cells degranulation as a consequence of a noxa of the peripheral nerve. The use according to claim 1, characterized in that these aminoalcohols residues R 2 and/or R 3 are selected from the group consisting of -CH 2 CH 2 -OH and The use according to claim 2, characterized in that when R 2 and/or R 3 are then the N-acylderivative is optically active or raceme. The use according to claim 1, characterized in that is the acyl radical of a monocarboxylic acid selected from the group consisting of saturated or unsaturated aliphatic acids, having from 2 to 20 carbon atoms, optionally substituted in the aliphatic chain with a amino or a hydroxy group, and monocarboxylic acids containing aromatic and/or heterocyclic groups. The use according to claim 4, characterized in that said aliphatic acids are selected from the group consisting of palmitic, oleic, lauric,stearic, myristic acids and their homologies having an amino or hydroxy substituent, and said acids containing aromatic and/or heterocyclic groups are selected from the group consisting of salicylic, acetylsalicylic, sulphosalicylic, benzoic, trimethoxybenzoic, α-lipoic, retinoic, thenoic, phenylanthranilic and hydroxyphenylacetic acids. The use according to claim 1, characterized in that said pathologies are the peripheral somatic and autonomic neuropathies, having traumatic, toxic or dysmetabolic origin. The use according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered orally in the form of dry powder and are selected from the group consisting of granulates, tablets, dragees, hard and soft jelly perles; or in the liquid form selected from the group consisting of suspensions, emulsions and aqueous solutions, wherein the active ingredients are present in the form of liposomes, optionally mixed with other lipids; or in semisolid form optionally obtained with polysaccharides aqueous suspensions solutions or emulsions wherein the active ingredient is present in the form of liposomes optionally mixed with other biologically acceptable lipids. The use according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered as dietetic integrating components in the form of dragees, tablets or oily perles, both for human beings and for animals. The use according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered topically, rectally and parenterally. The use according to claim 9, characterized in that said aminoalcohols N-acylderivatives are to be administered parenterally in the form of buffered aqueous solutions or emulsions, optionally containing the active ingredient in the form of liposomes optionally mixed with other biologically acceptable lipids, in the form of oily solutions, or in the form of a lyophilized product dispersable in the solvent at the moment of the administration. The use according to claim 9, characterized in that said aminoalcohols N-acylderivatives are to be administered rectally in the form of dispersions in a biologically acceptable phospholipidic excipient. The use according to claim 9, characterized in that said aminoalcohols N-acylderivatives are to be administered topically in the form of buffered solutions, gels or medicated plasters. The use according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered in doses ranging from 0.1 to 50 mg/kg. The use according to claim 13, characterized in that said doses range from 1 to 20 mg/kg. The use according to claim 13, characterized in that said doses are to be administered from once to twice a day, for a period of 3-4 weeks. The use according to claim 8, characterized in that said aminoalcohols N-acylderivatives are to be administered in daily doses ranging from 0.1 to 1 mg/kg. Claims for the following Contracting State : ES Process to obtain a pharmaceutical composition useful for the prevention or the treatment of human and animal pathologies involving a neurogenic endoneural oedema induced by mast cells degranulation as a consequence of a noxa of the peripheral nerve, said process consisting in mixing one or more aminoalcohols N-acylderivatives, having the formula: wherein R 2 is an alcoholic residue selected from a C 1 -C 20 linear or branched hydroxyalkyl optionally substituted in the alkyl chain with at least one aryl group, and a hydroxyaryl, optionally substituted on the aromatic ring with at least one linear or branched alkyl radical of from 1 to 20 carbon atoms, wherein R 3 is H or is = R 2 ; and wherein is an acyl radical of a monocarboxylic acid, with one or more pharmaceutically acceptable excipients. The process according to claim 1, characterized in that these aminoalcohols residues R 2 and/or R 3 are selected from the group consisting of -CH 2 CH 2 -OH and The process according to claim 2, characterized in that when R 2 and /or R 3 are then the N-acylderivative is an optically active or raceme. The process according to claim 1 characterized in that is the acyl radical of a monocarboxylic acid selected from the group consisting of saturated or unsaturated aliphatic acids, having from 2 to 20 carbon atoms, optionally substituted in the aliphatic chain with a amino or a hydroxy group, and monocarboxylic acids containing aromatic and/or heterocyclic groups. The process according to claim 4, characterized in that said aliphatic acids are selected from the group consisting of palmitic, oleic, lauric, stearic, myristic acids and their homologues having an amino or hydroxy substituent, and said acids containing aromatic and/or heterocyclic groups are selected from the group consisting of salicylic, acetylsalicylic, sulphosalicylic, benzoic, trimethoxybenzoic, α-lipoic, retinoic, thenoic, phenylanthranilic, hydroxyphenylacetic acids. The process according to claim 1, characterized in that said pathologies are the peripheral somatic and autonomic neuropathies having traumatic, toxic or dysmetabolic origin. The process according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered orally in the form of dry powder and are selected from the group consisting of granulates, tablets, dragees, hard or soft jelly perles; or in the liquid form selected from the group consisting of suspensions, emulsions and aqueous solutions, wherein the active ingredients are present in the form of liposomes, optionally mixed with other lipids; or in semisolid form optionally obtained with polysaccharides, aqueous suspensions, solutions or emulsions wherein the active ingredient is present in the form of liposomes optionally mixed with other biologically acceptable lipids. The process according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered as dietetic integrating components in the form of dragees, tablets or oily perles, both for human beings and for animals. The process according to claim 1, characterized in that said aminoalcohols N-acylderivatives are to be administered topically, rectally and parenterally. The process according to claim 9, characterized in that said aminoalcohols N-acylderivatives are to be administered parenterally in the form of buffered aqueous solutions or emulsions, optionally containing the active ingredient in the form of liposomes optionally mixed with other biologically acceptable lipids, in the form of oily solutions, or in the form of a lyophilized product dispersable in the solvent at the moment of the administration. The process according to claim 9, characterized in that said aminoalcohols N-acylderivatives are to be administered rectally in the form of dispersions in a biologically acceptable phospholipidic excipient. The process according to claim 9, characterized in that said aminoalcohols N-acylderivatives are to be administered topically in the form of buffered solutions, gels or medicated plasters. The process according to claim 1, characterized in that said aminalcohols N-acylderivatives are to be administered in dosages ranging from 0.1 to 50 mg/kg. The process according to claim 13, charcterized in that said doses range from 1 to 20 mg/kg. The process according to claim 13, characterized in that said doses are to be administered from once to twice a day, for a period of 3-4 weeks. The process according to claim 8, characterized in that said aminoalcohols N-acylderivatives are to be administered in daily doses ranging from 0.1 to 1 mg/kg.

Inventors: DELLA VALLE FEDERICA, DELLA VALLE FRANCESCO, LORENZI SILVANA, DELLA VALLE, FEDERICA, DELLA VALLE, FRANCESCO, LORENZI, SILVANA
Applicants: LIFEGROUP SPA, LIFEGROUP S.P.A.
Application year: 1993
Main classification: A61K31
IPC codes: C07D333, A61K31, A61K9, C07D339, C07C233, A61P25