ENANTIOMERICALLY PURE beta -D-DIOXOLANE NUCLEOSIDES WITH SELECTIVE ANTI-HEPATITIS B VIRUS ACTIVITY

Abstract: The invention is a method for the treatment of humans infected with HBV that includes administering an HBV treatment amount of an enatiomerically pure beta -D-dioxolanyl purine nucleoside of formula (I), wherein R is OH, Cl, NH2 or H, and X is selected from the group consisting of alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt.

Description: ENANTIOMERICALLY PURE jS-D-DIOXOLANE NUCLEOSIDES WITH SELECTIVE ANTI-HEPATITIS B VIRUS ACTIVITY Background of the Invention This invention is in the area of methods for the treatment of hepatitis B virus (also referred to as "HBV") that includes administering an effective amount of one or more of the active compounds disclosed herein, or a pharmaceutically acceptable derivative or prodrug of one of these compounds. HBV is second only to tobacco as a cause of human cancer. The mechanism by which HBV induces cancer is unknown, although it is postulated that it may directly trigger tumor development, or indirectly trigger tumor development through chronic inflammation, cirrhosis, and cell regeneration associated with the infection. Hepatitis B virus has reached epidemic levels worldwide. After a two to six month incubation period in which the host is unaware of the infection, HBV infection can lead to acute hepatitis and liver damage, that causes abdominal pain, jaundice, and elevated blood levels of certain enzymes. HBV can cause fulminant hepatitis, a rapidly progressive, often fatal form of the disease in which massive sections of the liver are destroyed. Patients typically recover from acute viral hepatitis. In some patients, however, high levels of viral antigen persist in the blood for an extended, or indefinite, period, causing a chronic infection. Chronic infections can lead to chronic persistent hepatitis. Patients infected with chronic persistent HBV are most common in developing countries. By mid-1991, there were approximately 225 million chronic carriers of HBV in Asia alone, and worldwide, almost 300 million carriers. Chronic persistent hepatitis can cause fatigue, cirrhosis of the liver, and hepatocellular carcinoma, a primary liver cancer. In western industrialized countries, high risk groups for HBV infection include those in contact with HBV carriers or their blood samples. The epidemiology of HBV is in fact very similar to that of acquired immunodeficiency syndrome, which accounts for why HBV infection is common among patients with AIDS or HIV-associated infections. However, HBV is more contagious than HIV. A human serum-derived vaccine has been developed to immunize patients against HBV. Vaccines have been produced through genetic engineering. While the vaccine has been found effective, production of the vaccine is troublesome because the supply of human serum from chronic carriers is limited, and the purification procedure is long and expensive. Further, each batch of vaccine prepared from different serum must be tested in chimpanzees to ensure safety. In addition, the vaccine does not help the patients already infected with the virus. Daily treatments with α-interferon, a genetically engineered protein, has also shown promise. However, to date there is no known pharmaceutical agent that effectively inhibits the replication of the virus in humans. In light of the fact that hepatitis B virus has reached epidemic levels worldwide, and has severe and often tragic effects on the infected patient, there remains a strong need to provide new effective pharmaceutical agents to treat humans infected with the virus that have low toxicity to the host. Therefore, it is another object of the present invention to provide a method and composition for the treatment of human patients or other hosts infected with HBV. Summary of the Invention In a preferred embodiment, the invention is a method for the treatment of humans infected with HBV that includes administering an HBV treatment amount of an enantiomerically pure 0-D-dioxolanyl purine nucleoside of the formula: wherein R is OH, Cl, NH2. or H, or a pharmaceutically acceptable salt or derivative of the compound, optionally in a pharmaceutically acceptable carrier or diluent. The compound wherein R is chloro is referred to as (-)-(2R,4R)- 2-amino-6-chloro-9-[ (2-hydroxymethyl)-I,3- dioxolan-4-yl]purine. The compound wherein R is hydroxy is (-)-(2R,4R)-9-[ (2-hydroxymethyl)-l,3- dioxolan-4-yl]guanine. The compound wherein R is amino is (-)-(2R,4R)-2-amino-9-[(2-hydroxymethyl)- l,3-dioxolan-4-yl]adenine. The compound wherein R is hydrogen is (-)-(2R,4R)-2-amino- 9-[ (2-hydroxymethyl)-l,3-dioxolan-4-yl] urine. The absolute configuration of these compounds has not been determined by crystallography. Designations are based on comparison of the structure to the configuration of the parent sugar used to make the compound. In another embodiment, an effective amount of the 0-L-dioxolanyl purine nucleoside enantiomer, or a racemic mixture of the /S-L- and β-D-dioxolanyl purine nucleoside is administered to the patient. It has been discovered that the EC 50 for ACPD ( (-)-(2R,4R)-2-amino-6-chloro-9- [ (2-hydroxymethyl)-l,3-dioxolan-4-yl]purine) ; and DAPD ( (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl)-l,3- dioxolan-4-yl]adenine) ) for HBV DNA replication intermediates or HBV virion synthesis inhibition is close to 0.1 μM. No marked cytotoxicity was noted for DAPD, ACPD, or DG ( (-)-(2R,4R)-9-[ (2- hydroxymethyl)-l,3-dioxolan-4-yl]guanine) when tested up to 300 μM in 2.2.15 cells. These three purine nucleosides were significantly non-toxic to yeloid and erthroid cells in clonogenic assays (IC 50 = 50 to greater than 100, as compared to AZT IC 50 of 1 μM) . It has also been discovered that DG, DAPD, ACPD, and APD ((-)-(2R,4R)-2-amino-9-[(2-hydroxymethyl) -l,3-dioxolan-4-yl]purine) are not inhibitors of enzymes involved in purine and pyrimidine biosynthesis, such as adenosine deaminase, purine nucleoside phosphorylase, hypoxanthine-guanosine phosphoribosyl transferase, adenosine kinase, inosine kinase, cytidine kinase, xanthine oxidase, aldehyde oxidase and xanthine dehydrogenase, when tested at a concentration up to 1 mM. The disclosed 0-dioxolane purine nucleosides, or their pharmaceutically acceptable derivatives or salts or pharmaceutically acceptable formulations containing these compounds are useful in the prevention and treatment of HBV infections and other related conditions such as anti-HBV antibody positive and HBV-positive conditions, chronic liver inflammation caused by HBV, cirrhosis, acute hepatitis, fulminant hepatitis, chronic persistent hepatitis, and fatigue. These compounds or formulations can also be used prophylactically to prevent or retard the progression of clinical illness in individuals who are anti-HBV antibody or HBV-antigen positive or who have been 5 exposed to HBV. In one embodiment of the invention, one or more of the active compounds is administered in an alternative fashion with one or more other anti-HBV agents, to provide effective anti-HBV treatment. Examples of anti-HBV agents that can be used in alternation therapy include but are not limited to the enantismer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-1-yl)- 1,3-oxathiolane ("FTC", see W092/14743) , its physiologically acceptable derivative, or physiologically acceptable salt; (-)-enantismer or racemic mixture of 2-hydroxymethyl-5-(σytosin- l-yl)-l,3-oxathiolane (also referred to as "BCH-189" or 3TC, see EPA Publication No. 0 382 526) , its physiologically acceptable derivative, or physiologically acceptable salt; an enantismer or racemic mixture of 2'-fluoro-5-iodo- arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2 , -fluoro-5-ethyl-arabinosyluracil (FEAU) ; carbovir, or interferon. Any method of alternation can be used that provides treatment to the patient. Nonlimiting examples of alternation patterns include 1-6 weeks of administration of an effective amount of one agent followed by 1-6 weeks of administration of an effective amount of a second anti-HBV agent. The alternation schedule can include periods of no treatment. In another embodiment, the active compound or its derivative or salt can be administered in combination with another anti-HBV agent, including those listed above. In general, during alternation therapy, an effective dosage of each anti-HBV agent is administered serially, whereas in combination therapy, a reduced dosage of two or more anti-HBV agents are administered together. The dosages will depend on absorption, inactivation, and excretion rates of the drug as well as other factors known to those of skill in the art. It is to be noted that dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens and schedules should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions. Brief Description of the Figures Figure 1 is an illustration of the method of preparation of a variety of enantiomerically pure jβ-D-dioxolanyl purine nucleosides. Figure 2 is a graph of the effect of purine dioxolanes and AZT on colony formation of human erythroid (BFU-E) precursor cells, as measured in terms of percent of cells of control versus the log of the concentration of test drug (AZT, 3'-azido- deoxy-thymidine; APD, (-)-(2R,4R)-2-amino-9- [ (2-hydroxymethyl)-l, 3-dioxolan-4-yl]purine; ACPD, (-)-(2R,4R)-2-amino-6-chloro-9-[ (2-hydroxymethyl) -l,3-dioxolan-4-yl]purine; DG, (-)-(2R,4R)-9-[ (2- hydroxymethy1)-1,3-dioxolan-4-y1]guanine; DAPD , (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl)-l,3- dioxolan-4-yl]adenine) . Figure 3 is a graph of the effect of purine dioxolanes and AZT on colony formation of human granulocyte-macrophage precursor cells, as measured in terms of percent of cells of control versus the log of the concentration of test drug. For abreviations used, see description of Figure 2. Figure 3 is a graph of the percent inhibition of HBV DNA replication in 2.2.15 cells on day 9 in varying concentrations of test compounds. For abreviations used, see description of Figure 2 ((- )-FTC is (-)-2-hydroxymethyl-5-(5-fluorocytosin-l- yl)-l,3-oxathiolane) . See Table * for corresponding data. Figure 4 is a graph of the uptake of 5 μM of tritiated (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl) -l,3-dioxolan-4-yl]adenine (DAPD) in Hep2G cells. Extract was obtained at four hours after exposing cells to DAPD. lOOOdmp/pmol; 80 μL injected. Figure 5 is a graph of the uptake of 5 μM of tritiated (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl) -l,3-dioxolan-4-yl]adenine (DAPD) in Hep2G cells. Extract was obtained at twelve hours after exposing cells to DAPD. lOOOdmp/pmol; 145 μL injected. Detailed Description of the invention As used herein, the term "enantiomerically pure" refers to a nucleoside composition that includes at least approximately 95%, and preferably 97%, of a single enantiomer of that nucleoside. The invention as disclosed herein is a method and composition for the treatment of HBV infection, in humans or other host animals, that includes administering an effective amount of one or more of the above-identified compounds, or a physiologically acceptable derivative, including a 5' and or N* alkylated or acylated derivative, or a physiologically acceptable salt thereof, optionally in a pharmaceutically acceptable carrier. The compounds of this invention either possess anti-HBV activity, or are metabolized to a compound or compounds that exhibits antiretroviral activity. In another embodiment, the invention includes a method for the treatment of humans infected with HBV that includes administering an HBV treatment amount of a prodrug of the specifically disclosed enantiomerically pure β-D-dioxolanyl purine nucleosides. A prodrug, as used herein, refers to a pharmaceutically acceptable derivative of the specifically disclosed nucleoside, that is converted into the nucleoside on administration in vivo, or that has activity in itself. Nonlimiting examples are pharmaceutically acceptable salts (alternatively referred to as "physiologically acceptable salts"), and the 5' and N* acylated or alkylated derivatives of the active compound (alternatively referred to as "physiologically or pharmaceutically acceptable derivatives") . In one embodiment, the acyl group is a carboxylic acid ester in which the non-carbonyl moiety of the ester group is selected from straight, branched, or cyclic Cι-C 20 alkyl; alkoxyalkyl including methoxymethyl; aralkyl including benzyl; aryloxyalkyl such as phenoxymethyl; aryl including phenyl optionally substituted with halogen, C, to C 4 alkyl or C, to C 4 alkoxy; a dicarboxylic acid such as succinic acid; sulfonate esters such as alkyl or aralkyl sulphonyl including methanesulfonyl; and the mono, di and triphosphate esters. As used herein, the term alkyl specifically includes but is not limited to methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, isobutyl, sec-butyl, t-butyl, isopentyl, amyl, t-pentyl, cyclopentyl, and cyclohexyl. As used herein, the term acyl specifically includes but is not limited to acetyl, propionyl, butyryl, pentanoyl, 3-methylbutyryl, hydrogen succinate, 3-chlorobenzoate, benzoyl, acetyl, pivaloyl, esylate,propionyl, valeryl, caproic, caprylic, capric, lauric, myriεtic, palmitic, stearic, and oleic. The nucleoside can also be provided as a 5' ether lipid, as disclosed in the following references, which are incorporated by reference herein: Kucera, L.S., N. Lyer, E. Leake, A. Raben, Modest E.J., D. L.W. , and C. Piantadosi. 1990. Novel membrane-interactive ether lipid analogs that inhibit infectious HIV-1 production and induce defective virus formation. AIDS Res Hum Retroviruses. 6:491-501; Piantadosi, C. , J. Marasco C.J., S.L. morris-Natschke, K.L. Meyer, F. Gu us, J.R. Surles, K.S. Ishaq, L.S. Kucera, N. lyer, CA. Wallen, S. Piantadosi, and E.J. Modest. 1991-Synthesis and evaluation of novel ether lipid nucleoside conjugates for anti-HIV activity. J Med Chem. 34:1408-1414; Hostetler, K.Y., D.D. Richman, D.A. Carson, L.M. Stuhmiller, G.M. T. van Wijk, and H. van den Bosch. 1992. Greatly enhanced inhibition of human immunodeficiency virus type 1 replication in CEM and HT4-6C cells by 3l-deoxythymidine diphosphate dimyristoylglycerol, a lipid prodrug of 31-deoxythymidine. Antimicrob Agents Chemother. 36:2025-2029; Hostetler, K.Y., L.M. Stuhmiller, H.B. Lenting, H. van den Bosch, and D.D. Richman. 1990. Synthesis and antiretroviral activity of phospholipid analogs of azidothymidine and other antiviral nucleosides. J. Biol Chem. 265:6112-7. The β-dioxolanyl purine nucleoside can be converted into a pharmaceutically acceptable ester by reaction with an appropriate esterifying agent, for example, an acid halide or anhydride. The nucleoside or its pharmaceutically acceptable derivative can be converted into a pharmaceutically acceptable salt thereof in a conventional manner, for example, by treatment +with an appropriate base. The ester or salt can be converted into the parent nucleoside, for example, by hydrolysis. The active compound can be provided in the form of pharmaceutically acceptable salts. As used herein, the term pharmaceutically acceptable salts or complexes refers to salts or complexes of the nucleosides that retain the desired biological activity of the parent compound and exhibit minimal, if any, undesired toxicological effects. Nonlimiting examples of such salts are (a) acid addition salts formed with inorganic acids (for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, and the like) , and salts formed with organic acids such as acetic acid, oxalic acid, tartaric acid, succinic acid, malic acid, ascorbic acid, benzoic acid, tannic acid, pamoic acid, alginic acid, polyglutamic acid, naphthalenesulfonic acids, naphthalenedisulfonic acids, and polygalacturonic acid; (b) base addition salts formed with cations such as sodium, potassium, zinc, calcium, bismuth, barium, magnesium, aluminum, copper, cobalt, nickel, cadmium, sodium, potassium, and the like, or with an organic cation formed from N,N-dibenzylethylene-diamine, ammonium, or ethylenediamine; or (c) combinations of (a) and (b) ; e.g., a zinc tannate salt or the like. Modifications of the active compound, specifically at the N* and 5'-0 positions, can affect the bioavailability and rate of metabolism of the active species, thus providing control over the delivery of the active species. The active compound, or pharmaceutically acceptable derivative or salt thereof can also be mixed with other active materials that do not impair the desired action, or with materials that supplement the desired action, such as antibiotics, antifungals, antiinflammatories, or other antivirals, including anti-HBV or anti-HIV agents. I. Preparation of Enantiomerically Pure Dioxolane Nucleosides Enantiomerically pure β-D-dioxolane-nucleosides can be prepared as disclosed in detail below, and as described in PCT/US91/09124. The process involves the initial preparation of (2R,4R)- and (2R,4S)-4-acetoxy-2-(protected-ox-ymethyl)-dioxolan e from 1 ,6-anhydromannose, a sugar that contains all of the necessary stereochemistry for the enantiomerically pure final product, including the correct diastereomeric configuration about the 1 position of the sugar (that becomes the 4'-position in the later formed nucleoside) . The (2R,4R)- and (2R,4S)-4-acetoxy-2-(protected- oxymethyl)-dioxolane is condensed with a desired heterocyclic base in the presence of SnCl 4 , other Lewis acid, or trimethylsilyl triflate in an organic solvent such as dichloroethane, acetonitrile, or methylene chloride, to provide the stereochemically pure dioxolane-nucleoside. In preparing enantiomerically pure dioxolane nucleosides, care should be taken to avoid strong acidic conditions that would cleave the dioxolane ring. Reactions should be performed, if possible, in basic or neutral conditions, and when acidic conditions are necessary, the time of reaction should be minimized. Racemic mixtures of dioxolane purine nucleosides can be prepared as described in EPA Publication No. 0 382 526. The β-L-enantiomer can be isolated from the racemic mixture by known methods, including through the use of a chiral HPLC column. Figure I and Example 1 set out a process for the preparation of the active compounds. The starting material, compound 1, is prepared as disclosed in PCT/US91/09124 (compound 8 in that application) . 2,6-Disubstituted purine derivatives were synthesized by the condensation of acetate 1 with the silylated 6-chloro-2-fluoropurine, which gave a mixture of 2 and 3. The initially formed N 7 -isomer was again converted to the N 9 -isomer during stirring overnight at room temperature. The analytical sample was obtained from the separation of α,β-mixture to the individual isomers 2 and 3 by a preparative TLC using (19:1) as the developing solvents. However, for the purpose of preparing the final products 10-15, the mixture of 2 and 3 was treated with NH 3 in DME (Robins, M.i.; Vznanski, B. Nucleic acid related compounds. 34. Non-aqueous Diazotization with tert-Butyl nitrite. Introduction of fluorine, chlorine, and bromine at C-2 of purine nucleosides. Can . J. Chem . 1981, 2608) to give a mixture of 10-13, which was separated to the individual isomers 4 (24%) , 5 (18.6%;), 6 (25.89.,) and 7 (16%,). The guanine 8 and 2,6-diamino 9 derivatives were prepared by the treatment of 4 with 2-mercaptoethanol/NaOMe and ammonia in ethanol, respectively. The free nucleosides 10-15 were obtained upon treatment of the corresponding 5'-silylated nucleosides with n-Bu 4 NF in good yields. The α-isomers 12 and 13 were prepared by the similar procedure as the β-isomers. Example 1 Preparation of Enantiomerically Pure β-D-Dioxolanyl Purine Nualeosides (2R,4R) and (2R,4S)-9-[[2-[ (tert- Butyldiphenylsilyl) oxylmethyl]- l,3-dioxolan-4- yl]-6-chloro-2-fluoropurine (2 and 3). A mixture of 2-fluoro-6-chloropurine (4.05 g, 23.47 mmol) and ammonium sulfate (catalytic amount) in hexamethyldisilazane (940 L) was refluxed for 2 hours. The resulting solution was concentrated under anhydrous conditions to yield silylated 2-fluoro-6-chloropurine as a white solid. To a cooled (OOC) and stirred solution of silylated 2-fluoro-6-chloropurine (5.69 g, 23.69 mmol) and compound 1 (7.84 g, 19.57 mmol) in dry methylene chloride (175 mi) was added TMSOTF (4.41 mL, 23.44 mmol) . The reaction mixture was warmed to room temperature and stirred for 16 hours, during which time, all the initially formed N 7 condensed product was converted to N 9 -isomer. The reaction mixture was quenched with saturated NaHC0 3 solution (50 mi) and stirred for an additional 20 minutes at room temperature, evaporated to dryness under reduced pressure. The residue was dissolved in ethyl acetate (200 mi) , washed with water and brine, dried (anhydrous Na 2 S0 4 ) , filtered and evaporated to give a solid residue, which was purified by silica gel column chromatography (20% EtOAc in hexanes) to afford a mixture of β-anomer 8 and -anomer 9 (1.3:1; β/α) as a white crystalline solid (6.30 g, 62.8%). The analytical sample was purified by preparative TLC using CH 2 CL 2 -acetone (19:1) as the 5 developing system to give 2 (R f = 0.50) and 3 (Rj = 0 . 55 ) for NMR characterization : UV (MeOH) μ,,^ 269 . 0 nm. (-)-(2R,4R)-2-Amino-9-[[2-[(tert-butyldiphenylsilyl )oxylmethyl]-l,3-dioxolan-4-yl]-6-chloropurine (4) , (-)-(2R,4R)-9-[[2-[ (tert-Butyldiphenylsilyl) oxγ]methyl]-l,3-dioxolan-4-yl]-2-fluoroadenine(5) , (+)-(2R,4S)-2-Amino-9-[[2-[(tert- butyldiphenylsilyl) oxylmethyl]-1,3-dioxolan- 4-yl]-6-chloropurine (6) and (+)-(2R,4S)-9- [[2-[ (tert-Butyldiphenylsilyl)oxylmethyl]- l,3-dioxolan-4-yl]-2-fluoroadenine (7) . Dry ammonia gas was bubbled into a stirred solution of 2 and 3 (6.25 g, 12.18 mmol) in DME (125 mL) overnight) . The solvent was evaporated under reduced pressure and the residue was subjected to chromatographic separation of the four compounds on a silica gel column (20-30% ethyl acetate in CHjCLj) . 4 (R, = 0.35, 1.49 g, 24%): a white crystalline solid. UV (MeOH) X ^ 309.5 nm. Anal. C, H, CI, N. 5 (R, = 0.21, 1.12 g, 18.6%): colorless needles. UV (MeOH)A λ ^ 261.0, 268.0 (sh) n . Anal. (C^H^FN^Si) C, H, F, N. 6 (R f = 0.43, 1.60 g, 25.76%): a white crystalline solid. UV (MeOH) λ, ^ 261.0, 269.0 (sh) nm. Anal. (C^H^FNgOaSi) C, H, F, N. 7 (R f= 0.12, 0.96 g, 16%), a 25 microcrystalline solid. UV (methanol) λ mιa 261.0, 269.0 (sh) nm. Anal. (C^FNsOaSi) C, H, F, N. (-)-(2R,4R)-2-Amino-6-chloro-9-[(2-hydroxymethyl)- 1,3-dioxolan-4-yl] urine (10). A solution of 4 (0.46 g, 0.91 mmol) in THF (20 mL) was treated with 1 M n-Bu 4 NF/THF (1.1 mL, 1.1 mmol) to give 10 (R, = 0.50, 0.21 g, 84%) as a crystalline solid, which was recrystallized from MeOH: UV (H 2 0) λ,,, ^ 307.0 nm (ε8,370) (pH 7), 307.5 (ε8,590) (pH 2), 307.0 (ε8, 800) (pH 11). Anal. (C,H 10 CIN 5 O 3 ) C, H, Cl, N. (-)-(2R,4R)-2-Pluoro-9-[(2-hydroxymethyl)-l,3- dioxolan-4- yl]adenine (11) . A solution of 5 (0.56 g, 1.12 mmol) in THF (20 mL) was treated with 1 M n-Bu 4 NF/THF (1.35 mL, 1.35 mmol) to furnish 22 (0.24 g, 85%) as a white crystalline solid, which was recrystallized from MeOH: UV (H 2 0) λ m „ 260.8 nm (εl7,010), 268.5 (sh) nm (εl3,510) (pH 7), 261. 0 (εl6,390), 268.5 (sh) (εl3,300) (pH 2), 260.8 (εl6,700), 268.5 (sh) (εl3,200) (pH 11). Anal. C, H, F, N. (-)-(2R,4R)-9-[(2-Hydroxymethyl)-l,3-dioxolan-4-yl] guanine (14). A mixture of 4 (0.29 g, 0. 57 mmol), HSCH 2 CH 2 OH (0.51 mL) and 1.0 M NaOMe/MeOH (11.5 mL) in MeOH (20 L) was refluxed for 3 hours. The reaction mixture was cooled and neutralized with glacial acetic acid. The solution was evaporated to dryness, and then the residue was triturated with CHCI 3 , filtered and the filtrate was taken to dryness to give crude compound 8 (0.21 g, 75%), which without further purification was subjected to desilylation to give compound 3 (0.07 g, 61%) as a microcrystalline solid, which was recrystallized from MeOH: UV (H 2 0) λ m „ 252.0 (ε8,730) (pH 7), 254.4 (εl2,130), 277.5 (sh) (ε8,070) (pH 2) , 264.3 (ε.10,800) (pHll) . Anal. (C 9 H n N s 0 4 ) C, H, N. (-)-(2R,4R)-2-Amino-9-[(2-hydroxymethyl)-1, 3-dioxolan-4-yl]adenine (15) . A steel bomb was charged with compound 4 (0.28 g, 0.55 mmol), anhydrous ethanol (20 mL) saturated with NH 3 , and heated at 90°C for 6 hours. After cooling, the compound 9 (0.26 g, 95%) obtained on evaporated of the solvent in vacuo. and then desilylated according to the same procedure described for preparation of 12 to give 15 (0.10 g, 75%) as white micro needles, recrystallized from MeOH: UV (H 2 0) λ m „ 279.0 nm (ε 8,040) (pH 7), 290.0 (ε 7,070) (pH 2), 278.8 (ε 7,580) (pH 11). Anal. (CAjNβQj) C, H, N. (-)-(2R,4R)-2-Amino-9-[ (2-hydroxymethyl)- 1, 3-dioxolan-4-yl]purine can be prepared by reduction of compound 10 using a variety of reducing agents, including palladium on carbon and hydrogen gas or tributyltin hydride and azabisisobutyronitrile. II. Anti-HBV Activity of Dioxolane Nucleosides The ability of β-D-dioxolane-nucleosides to inhibit HBV can be measured by various experimental techniques. The assay used herein to evaluate the ability of the disclosed compounds to inhibit the replication of HBV is described in detail in Korba and Gerin, Antiviral Res. 19: 55-70 (1992). For purposes of illustration only, and without limiting the invention, below is provided the results of the evaluation of toxicity and anti-HBV activity of (-)-(2R,4R)-2-amino-6-chloro-9-[ (2-hydroxymethyl)- l,3-dioxolan-4-yl]purine; (-)-(2R,4R)-2-amino-9- [ (2-hydroxymethy1)-I,3-dioxolan-4-y1]adenine; and (-)-(2R,4R)-9-1(2-hydroxymethyl)-1,-3- dioxolan-4-yl]quanine. The other compounds disclosed herein are evaluated similarly. The antiviral evaluations were performed on two separate passages of cells, two cultures per passage (4 cultures total) . All wells, in all plates, were seeded at the same density and at the same time. Due to the inherent variations in the levels of both intracellular and extracellular HBV DNA, only depressions greater than 3.0-fold (for HBV virion DNA) or 2.5-fold (for HBV DNA replication intermediates) from the average levels for these HBV DNA forms in untreated cells are generally considered to be statistically significant [P<0.05] (Korba and Gerin, Antiviral Res. 19: 55-70, 1992). The levels of integrated HBV DNA in each cellular DNA preparation (which remain constant on a per cell basis in these experiments) were used to calculate the levels of intracellular HBV DNA forms, thereby eliminating technical variations inherent in the blot hybridization assays. Typical values for extracellular HBV virion DNA in untreated cells range from 50 to 150 pg/ml culture medium (average of approximately 76 pg/ml) . Intracellular HBV DNA replication intermediates in untreated cells range from 50 to 100 pg/ug cell DNA (average approximately 74 pg/ug cell DNA) . In general, depressions in the levels of intracellular HBV DNA due to treatment with antiviral compounds are less pronounced, and occur more slowly, than depressions in the levels of HBV virion DNA. For reference, the manner in which the hybridization analyses were performed for these experiments results in an equivalence of approximately 1.0 pg intracellular HBV DNA/ug cellular DNA to 2-3 genomic copies per cell and 1.0 pg of extracellular HBV DNA/ml culture medium to 3 x 10 s viral particles/ml. Toxicity analyses were performed in order to assess whether any observed antiviral effects are due to a general effect on cell viability. The method used was based on the uptake of neutral red dye, a standard and widely used assay for cell viability in a variety of virus-host systems, including HSV (herpes simplex virus) and HIV. Details of the procedure are provided in the toxicity table legends. The test compounds were used in the form of 40 M stock solutions in DMSO (frozen on dry ice) . Daily aliquots of the test samples were made and frozen at -20°C so that each individual aliquot would be subjected to a single freeze-thaw cycle. The daily test aliquots were thawed, suspended into culture medium at room temperature and immediately added to the cell cultures. The compounds were tested at 0.0 and 1 μM for antiviral activity. The compounds were tested for toxicity at 5 concentrations up to 300 μM. The following abbreviations are used in the Tables: ACPD, (-)-(2R,4R)-2-amino-6- chloro- 9-[ (2-hydroxymethyl)-1,3-dioxolan-4-yl]purine; DAPD, (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl)- I,3-dioxolan-4-yl]adenine; and Dioxolane-G, (2R,4R)-9-[ (2-hydrox-ymethyl)-l,3-dioxalan-4-yl] guanine. Example 2 Toxicity Of Compounds The ability of the enantiomers of ACPD, DAPD, and dioxolane-G to inhibit the growth of virus in 2.2.15 cell cultures (HepG2 cells transformed with hepatitis virion) was 15 evaluated. As illustrated in Table 1, no significant toxicity (greater than 50% depression of the dye uptake levels observed in untreated cells) was observed for any of the test compounds at the concentrations used for the antiviral evaluations. The test compounds were not toxic to 2.2.15 cells at 100 μM. The compounds were moderately toxic at 300 μM, however, all three compounds exhibited less toxicity at this conentration than ddC. Toxicity analyses were performed in 96-well flat bottomed tissue culture plates. Cells for the toxicity analyses were cultured and treated with test compounds with the same schedule as used for the antiviral evaluations. Each compound was tested at 4 concentrations, each in triplicate cultures. Uptake of neutral red dye was used to determine the relative level of toxicity. The absorbance of internalized dye at 510 nM (As, 0 ) was used for the quantitative analysis. Values are presented as a percentage of the average A SJ0 values (+/- standard deviations) in 9 separate cultures of untreated cells maintained on the same 96-well plate as the test compounds. The percentage of dye uptake in the 9 control cultures on plate 40 was 100 +/- 3. At 150-190 μM 2',3'-ddC, a 2-fold reduction in dye uptake (versus the levels observed in untreated cultures) is typically observed in these assays (Korba and Gerin, Antiviral Res. 19: 55-70, 1992). Tabl* l. Toxicity analysis of test compounds in 2.2.15 cells. NEUTRAL RED DYE UPTAKE AT INDICATED DRUG CONCENTRATION (% OF CONTROL) NEUTRAL RED DYE UPTAKE AT INDICATED DRUG CONCENTRATION (% OF CONTROL) Exa ple 3 Anti-Hepatitis B virus Activity As indicated in Table 2, within normal variations, levels of HBV virion DNA and intracellular HBV replication intermediates [HBV RI] remained constant in the untreated cells over the challenge period. The positive treatment control, 2 ' ,3'-dideoxycytosine [2',3'-ddC], induced significant depressions of HBV DNA replication at the concentration used. Previous studies have indicated that 9-12 μM 2',3'-ddC, a 90% depression of HBV RI (relative to average levels in untreated cells) is typically observed in this assay system (Korba and Gerin, Antiviral Res. 19: 55-70, 1992) . All three test compounds were potent inhibitors of HBV replication, causing depression of HBV virion DNA and HBV RI to a degree comparable to, or greater than, that observed following treatment with 2',3'-ddC. Table 2 EFFE C T O F VARIOUS NUCLEOSIDES ON HBV PRODUCTION IN 2.2.15 CELLS Treatment Mono RI None DDC 10 μM 3.0 μM (-)- -2-NII2-6-C1- purine-dioxolane 1 . 0 μM (-) - -2-NH2-6-C1- purine-dioxolane 1.0 μM (-)- -2-NH2-6-C1- purine-dioxolane 1.0 μM (-)- -2-NH2-6-C1- purine-dioxolane 1.0 μM (-)- -2-NH2-6-C1- purine- ioxolane 1.0 μM (-)- -2-NH2-6-C1- purine-dioxolane * Analysis of intracellular HBV DNA was 24 hours following the 9th day of treatment. DNA in each cell DNA preparation were used o calculate the levels of episomal 3.2kB HBV genomes (MONO.) and HBV DNA replication intermediates [RI]- ** A "zero" indicates an undetectable level of HBV DNA, sensitivity cutoff was 0.1 pgfm]. Example 3 Toxicity in Human Erythroid (BFϋ-E) Precursor Cells Figure 2 is a graph of the effect of selected purine dioxolanes and AZT on colony formation of human erythroid (BFU-E) precursor cells, as measured in percent of control cells versus the concentration in μM. As indicated, the four purine dioxolanyl nucleosides tested, APD, (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl)-1, 3-dioxolan-4-yl]purine; ACPD, (-)-(2R,4R)-2-amino-6-chloro-9- [ (2-hydroxymethyl)-1,3-dioxolan-4-yl]purine; DG, (-)-(2R,4R)-9-[(2-hydroxymethyl)-l,3- dioxolan-4-yl]guanine; and DAPD, (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl)-1,3- dioxolan-4-yl]adenine) , appear to be significantly less toxic than AZT in this cell line. Example 4 Effect on Colony Formation of Human Granulocyte-Macrophage Precursor Cells Figure 3 is a graph of the effect of ACPD, DG, DAPG, DG and AZT on colony formation of human granulocyte-macrophage precursor cells, as measured in terms of percent of cells of control versus the log of the concentration of test drug. As indicated, the purine dioxolanyl nucleosides appear to be significantly less toxic, i.e., have a higher IC 50 , than AZT in this cell line. Example 5 Effect on HBV DNA Replication Figure 4 is a graph of the percent inhibition of HBV DNA replication in 2.2.15 cells on day 9 in varying concentrations of test compounds, using a narrower range of concentration than that used in Example 1. Table 3 provides the HBV virion and HBV RI EC 50 and EC^, cytotoxicity and selectivity index for DG, DAPG, ACPD, FTC, and DDC. Exa ple 6 Figure 5 is a graph of the uptake of 5 μM of tritiated (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl) -l,3-dioxolan-4-yl]adenine (DAPD) in HepG2 cells. Extract was obtained at four hours after exposing cells to DAPD (1000 dmp/pmol; 80 μL injected) . The data indicates that the compound is primarily metabolised intracellularly to the triphosphate form. Example 7 Figure 6 is a graph of the uptake of 5 μM of tritiated (-)-(2R,4R)-2-amino-9-[(2-hydroxymethyl) -l,3-dioxolan-4-yl]adenine (DAPD) in HepG2 cells. Extract was obtained at twelve hours after exposing cells to DAPD (1000 dmp/pmol; 145 μL injected) . The data indicates that after four hours of incubation with the tritiated compound, there are high intracellular levels of the triphosphate. IV. Preparation of Pharmaceutical Compositions The compounds disclosed herein and their pharmaceutically acceptable salts, prodrugs, and derivatives, are useful in the prevention and treatment of HBV infections and other related conditions such as anti-HBV antibody positive and HBV-positive conditions, chronic liver inflammation caused by HBV, cirrhosis, acute hepatitis, fulminant hepatitis, chronic persistent hepatitis, and fatigue. These compounds or formulations can also be used prophylactically to prevent or retard the progression of clinical illness in individuals who are anti-HBV antibody or HBV-antigen positive or who have been exposed to HBV. Humans suffering from any of these conditions can be treated by administering to the patient an effective amount of (-)-(2R,4R)-2-amino-6-chloro- 9-[ (2-hydroxymethyl)-l,3-dioxolan-4-yl]purine; (-)-(2R,4R)-9-[(2-hydroxymethyl)-l,3-dioxolan-4- yl]guanine; (-)-(2R,4R)-2-amino-9-[ (2- hydroxymethy1)-1,3-dioxolan-4-y1]adenine; or (-)-(2R,4R)-2-amino-9-[ (2-hydroxymethyl)- l,3-dioxolan-4-yl]purine or a pharmaceutically acceptable derivative or salt thereof, optionally in a pharmaceutically acceptable carrier or diluent. The active materials can be administered by any appropriate route, for example, orally, parenterally, intravenously, intradermally, subcutaneously, or topically, in liquid or solid form. The active compound is included in the pharmaceutically acceptable carrier or diluent in an amount sufficient to deliver to a patient a therapeutically effective amount without causing serious toxic effects in the patient treated. A preferred dose of the active compound for all of the above-mentioned conditions will be in the range from about 1 to 60 mg/kg, preferably 1 to 20 mg/kg, of body weight per day, more 5 generally 0.1 to about 100 mg per kilograin body weight of the recipient per day. The effective dosage range of the pharmaceutically acceptable derivatives can be calculated based on the weight of the parent nucleoside to be delivered. If the derivative exhibits activity in itself, the effective dosage can be estimated as above using the weight of the derivative, or by other means known to those skilled in the art. The compound is conveniently administered in unit any suitable dosage form, including but not limited to one containing 7 to 3000 mg, preferably 70 to 1400 mg of active ingredient per unit dosage form. A oral dosage of 50-1000 mg is usually convenient. Ideally the active ingredient should be administered to achieve peak plasma concentrations of the active compound of from about 0.2 to 70 μM, preferably about 1.0 to 10 μM. This may be achieved, for example, by the intravenous injection of a 0.1 to 5% solution of the active ingredient, optionally in saline, or administered as a bolus of the active ingredient. The concentration of active compound in the drug composition will depend on absorpeion, inactivation, and excretion rates of the drug as well as other factors known to those of skill in the art. It is to be noted that dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that the concentration ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed .composition. The active ingredient may be administered at once, or may be divided into a number of smaller doses to be administered at varying intervals of time. A preferred mode of administration of the active compound is oral. Oral compositions will generally include an inert diluent or an edible carrier. They may be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agentsuch as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring. When the dosage unit form is a capsule, it can contain, in addition to material of the above type, a liquid carrier such as a fatty oil. In addition, dosage unit forms can contain various other materials which modify the physical form of the dosage unit, for example, coatings of sugar, shellac, or other enteric agents. The active compound or pharmaceutically acceptable salt or derivative thereof can be administered as a component of an elixir, suspension, syrup, wafer, chewing gum or the like. A syrup may contain, in addition to the active compounds, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors. The active compound, or pharmaceutically acceptable derivative or salt thereof can.also be mixed with other active materials that do not impair the desired action, or with materials that supplement the desired action, such as antibiotics, antifungals, antiinflammatories, or other antivirals, including anti-HBV, anti-cytomegalovirus, or anti-HIV agents. Solutions or suspensions used for parenteral, intrader al, subcutaneous, or topical application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The parental preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic. If administered intravenously, preferred carriers are physiological saline or phosphate buffered saline (PBS) . In a preferred embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such fo ulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) are also preferred as pharmaceutically acceptable carriers. These may be prepared according to methods known to those skilled in the art, for example, as described in U.S. Patent No. 4,522,811 (which is incorporated herein by reference in its entirety) . For example, liposome formulations may be prepared by dissolving appropriate lipid(s) (such as stearoyl phosphatidyl ethanolamine, stearoyl phosphatidyl choline, arachadoyl phosphatidyl choline, and cholesterol) in an inorganic solvent that is then evaporated, leaving behind a thin film of dried lipid on the surface of the container. An aqueous solution of the active compound or its monophosphate, diphosphate, and/or triphosphate derivatives are then introduced into the container. The container is then swirled by hand to free lipid material from the sides of the container and to disperse lipid aggregates, thereby forming the liposomal suspension. V. Preparation of Phosphate Derivatives of J&-D-Dioxolane-Nucleosides Mono, di, and triphosphate derivative of E-D-dioxolane-nucleosides can be prepared as described below. The monophosphate can be prepared according to the procedure of Imai et al., J. Orσ. Chem.. 34(6), 1547-1550 (June 1969). For example, about 100 mg of β-D-dioxolane-nucleoside and about 280 μl of phosphoryl chloride are reacted with stirring in about 8 ml of dry ethyl acetate at about 0°C for about four hours. The reaction is quenched with ice. The aqueous phase is purified on an activated charcoal column, eluting with 5% ammonium hydroxide in a 1:1 mixture of ethanol and water. Evaporation of the eluant gives ammonium-(β-D-dioxolane- nucleoside)-5 '-monophosphate. The diphosphate can be prepared according to the procedure of Davisson et al., J. Orσ. Chem.. 52(9), 1794-1801 (1987) . β-D-Dioxolane-nucleosides can be prepared from the corresponding tosylate, that can be prepared, for example, by reacting the nucleoside with tosyl chloride in pyridine at room temperature for about 24 hours, working up the product in the usual manner (e.g., by washing, drying, and crystallizing it) . The triphosphate can be prepared according to the procedure of Hoard et al., J. Am. Chem. Soc.. 87(8), 1785-1788 (1965). For example, β-D-dioxolane-nucleoside is activated (by making a imidazolide, according to methods known to those skilled in the art) and treating with tributyl ammonium pyrophosphate in DMF. The reaction gives primarily the triphosphate of the nucleoside, with some unreacted monophosphate and some diphosphate. Purification by anion exchange chromatography of a DEAE column is followed by isolation of the triphosphate, e.g., as the tetrasodium salt. This invention has been described with reference to its preferred embodiments. Variations and modifications of the invention, enantiomerically pure β-D-dioxolane-nucleosides, will be obvious to those skilled in the art from the foregoing detailed description of the invention. It is intended that all of these variations and modifications be included within the scope of the appended claims.

Claims: I claim. 1. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of an enantiomerically pure β-D-dioxolanyl nucleoside of the structure: wherein R is OH, and X is selected from the group consisting of alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt, and wherein the compound is 95% free of the corresponding β-L enantiomer. 2. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of an enantiomerically pure β-D-dioxolanyl nucleoside of the structure: wherein R is NH 2 , and X is selected from the group consisting of alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt, and wherein the compound is 951k free of the corresponding β-L enantiomer. 3. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of an enantiomerically pure β-D-dioxolanyl nucleoside of the structure: wherein R is H or Cl, and X is selected from the group consisting of alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt, and wherein the compound is 95% free of the corresponding β-L enantiomer. 4. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the racemic mixture of a β-dioxolanyl nucleoside of the structure: wherein R is OH, and X is selected from the group consisting ot alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt. 5. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the racemic mixture of a β-dioxolanyl nucleoside of the structure: wherein R is NH 2 , and X is selected from the group consisting of alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt. 6. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the racemic mixture of a β-dioxolanyl nucleoside of the structure: wherein R is H or Cl, and X is selected trom the group consisting of alkyl, acyl, monophosphate, diphosphate, and triphosphate, or its pharmaceutically acceptable salt. 7. The method of claims 1, 2, 3, 4, 5, or 6 wherein the carrier is suitable for oral delivery. 8. The method of claims 1, 2, 3, 4, 5, or 6 wherein the carrier comprises a capsule. 9. The method of claims l, 2, 3, 4, 5, or 6 wherein the carrier is in the form of a tablet. 10. The method of claims l, 2, 3, 4, 5, or 6 wherein the administration is parenteral. 11. The method of claims 1, 2, 3, 4, 5, or 6, wherein the alkyl group is selected from the group consisting of methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, isobutyl, sec-butyl, t-butyl, isopentyl, amyl, t-pentyl, cyclopentyl, and cyclohexyl. 12. The method of claims l, 2, 3, 4, 5, or 6, wherein the acyl group is selected from the group consisting of acetyl, propionyl, butyryl, pentanoyl, 3-methylbutyryl, hydrogen succinate, 3-chlorobenzoate, benzoyl, acetyl, pivaloyl, mesylate, propionyl, valeryl, caproic, caprylic, capric, lauric, yristic, palmitic, stearic, and oleic. 13. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 1 in alternative dosages with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-l-yl)-l,3- oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5-iodo-arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2'-fluoro-5-ethyl- arabinosyluracil (FEAU) , carbovir, or interferon. 14. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 2 in alternative dosages with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-1-yl)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-1,3- oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5-iodo-arabinosyluracil (FIAU) ; 2'-fluoro-5-ethyl-arabinosyluracil (FEAU) , carbovir, or interferon. 15. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 3 in alternative dosages with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-1-yl)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5-iodo-arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2'-fluoro-5-ethyl- arabinosyluracil (FEAU) , carbovir, or interferon. 16. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 4 in alternative dosages with a compound selected from the group consisting of the (-)[]-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-l-yl)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2 -fluoro-5-iodo-arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2'-fluoro-5-ethyl- arabinosyluracil (FEAU) , carbovir, or interferon. 17. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 5 in alternative dosages with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-1-yl)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2 -fluoro-5-iodo-arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2'-fluoro-5-ethyl-arabinosyluracil (FEAU) , carbovir, or interferon. 18. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 6 in alternative dosages with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-1-yl)- I,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-1-yl)-1,3- oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5-iodo-arabinosyluracil (FIAU); an enantiomer or racemic mixture of 2 / -fluorc-5-ethyl-arabinosyluracil (FEAU) , carbovir, or interferon. 19. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 1 in combination with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-l-yl)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydrox-ymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5-iodo-arabinosyluracil (FIAU); an enantiomer or racemic mixture of 2 -fluoro-5-ethyl-arabinosyluracil (ΪΕAU) , carbovir, or interferon. 20. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 2 in combination with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-flucrocytosin-1-yl)-1,3-oxathi olane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5- (cytosin-l-yl)-l,3-oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5- iodo-arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2 / -fluoro-S-ethyl-arabinosyluracil (FEAU) , carbovir, or interferon. 21. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 3 in combination with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethy1-5-(5-fluorocytosin-1-y1)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2 / -fluoro-5-iodo-arabinosyluracil (FIAU); an enantiomer or racemic mixture of 2'-fluoro-5-ethyl- arabinosyluracil (FEAU) , carbovir, or interferon. 22. A method for the treatment of HIBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 4 in combination with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5- fluorocytosin-1-yl)-1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-I,3-oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5- iodo-arabinosyluracil (FIAU) ; an enantiomer or racemic mixture of 2'-fluoro-5-ethyl-arabinosyluracil (FEAU) , carbovir, or interferon. 23. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HBV treatment amount of the nucleoside of claim 5 in combination with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-1-yl)- I,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2 -fluoro-5-iodo-arabinosyluracil (FIAU); an enantiomer or racemic mixture of 2'-fluoro-5-ethyl- arabinosyluracil (FEAU) , carbovir, or interferon. 24. A method for the treatment of HBV infection in a human or other host animal, comprising administering an HAV treatment amount of the nucleoside of claim 6 in combination with a compound selected from the group consisting of the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(5-fluorocytosin-l-yl)- 1,3-oxathiolane; the (-)-enantiomer or racemic mixture of 2-hydroxymethyl-5-(cytosin-l-yl)-l,3- oxathiolane; an enantiomer or racemic mixture of 2'-fluoro-5-iodo-arabinosyluracil (FIAU); an enantiomer or racemic mixture of 2'-fluoro-5-ethyl- arabinosyluracil (FEAU) , carbovir, or interferon.

Inventors: SCHINAZI RAYMOND P, SCHINAZI, RAYMOND, P.
Applicants: UNIV EMORY, EMORY UNIVERSITY
Application year: 1993
Main classification: A61K31
IPC codes: A61K31, A61K38, A61P31, C07D473