METHODS AND COMPOSITIONS FOR THE IDENTIFICATION, CHARACTERIZATION, AND INHIBITION OF FARNESYLTRANSFERASE

Abstract: Disclosed are methods and compositions for the identification, characterization and inhibition of mammalian farnesyl protein transferases, enzymes involved in the farnesylation of various cellular proteins, including cancer related (ras) proteins such as p21ras. The nucleotide and amino acid sequences of the (alpha) and (beta) subunits of both rat and human farnesyl transferase are disclosed, as are methods and compositions for the preparation of farnesyl transferase by recombinant means, following the molecular cloning and co-expression of its two subunits, for assay and purification of the enzyme, as well as procedures for using the purified enzyme in screening protocols for the identification of possible anticancer agents which inhibit the enzyme and thereby prevent expression of proteins such as p21ras. Also disclosed is a family of compounds which act either as false substrates for the enzyme or as pure inhibitors and can therefore be employed for inhibition of the enzyme. The most potent inhibitors are ones in which phenylalanine occurs at the third position of a tetrapeptide whose amino terminus is cysteine. Improved inhibitors with defined structures and characteristics are also disclosed.

Description: DESCRIPTION METHODS AND COMPOSITIONS FOR THE IDENTIFICATION. CHARACTERIZATION, AND INHIBITION OF FARNESYLTRANSFERASE BACKGROUND OF THE INVENTION The present application is a continuation-in-part of co-pending U.S. Serial Number 07/935,087, filed August 24, 1992; which is a continuation-in-part of U.S. Serial Number 07/822,011, filed January 16, 1992; which is a continuation-in-part of PCT application, US 91/02650, filed April 18, 1991; which is a continuation-in-part of U.S. Serial Number 07/615,715, filed November 20, 1990, and which issued as U.S patent 5,141,851 on August 25, 1992; and which itself is a continuation-in-part of U.S. Serial Number 07/510,706, filed April 18, 1990. The U.S. Government may own certain rights in the present invention pursuant to NIH grant number 5-PO1-HL20948. 1. Field of the Invention This invention relates generally to improved peptide-based inhibitors of farnesyltransferase, the enzyme responsible for the farnesylation of p21 rϊ protein, and more particularly relates to peptide-based "pure" inhibitors having improved characteristics and structures. Improvements are based on the inventors' discovery of structural characteristics that ensure that a peptide inhibitor will exhibit "pure" inhibitor characteristics, which provide important guidelines for inhibitor design that will allow cellular uptake while preserving inhibitory capabilitites. 2. Description of the Related Art In recent years, some progress has been made in the elucidation of cellular events lending to the development or progression of various types of cancers. A .great amount of research has centered on identifying genes which are altered or mutated in cancer relative to normal cells. In fact, genetic research has led to the identification of a variety of gene families in which mutations can lead to the development of a wide variety of tumors. The ras gene family is a family of closely related genes that frequently contain mutations involved in many human tumors, including tumors of virtually every tumor group (see, e.g., Bos, 1989) . In fact, altered ras genes are the most frequently identified oncogenes in human tumors (Barbacid, 1987) . The ras gene family comprises three genes, H-ras, K-ras and N-ras, which encode similar proteins with molecular weights of about 21,000 (Barbacid, 1987) . These proteins, often termed p21 ras , comprise a family of GTP-binding and hydrolyzing proteins that regulate cell growth when bound to the inner surface of the plasma membrane (Hancock, et al . , 1989; Scheler et al . , 1989) . Overproduction of P21 ras proteins or mutations that abolish their GTP-ase activity lead to uncontrolled cell division (Gibbs et al . , 1989) . However, the transforming activity of ras is dependent on the localization of the protein to membranes, a property thought to be conferred by the addition of farnesyl groups (Hancock et al . , 1989; Casey et al . , 1989) . A precedent for the covalent isoprenylation of proteins had been established about a decade ago when peptide mating factors secreted by several fungi were shown to contain a farnesyl group attached in thioether linkage to the C-terminal cysteine (Kamiya et al . , 1978; 1979; Sakagami et al . , 1981) . Subsequent studies with the mating a-factor from Saccharomyces cerevisiae and farnesylated proteins from animal cells have clarified the mechanism of In each of these proteins the farnesyl. ;ed cysteine is initially the fourth residue from the C terminus (Hancock, et al. , 1989; Scheler et al . , 1989; Gutierrez et al . , 1989) . Immediately after translation, in a sequence of events whose order is not yet totally established, a farnesyl group is attached to this cysteine, the protein is cleaved on the C-terminal side of this residue, and the free COOH group of the cysteine is methylated (Hancock et al . , 1989; Gutierrez et al . , 1989; Lowry et al . , 1989; Clarke et al. , 1988) . All of these reactions are required for the secretion of active a-factor in Saccharomyces (Scheler et al . , 1989) . Most, if not all, of the known p21 ras proteins contain the cysteine prerequisite, which is processed by farnesylation, proteolysis and COOH-methylation, just as with the yeast mating factor (Hancock et al . , 1989; Scheler et al . , 1989; Gutierrez et al. , 1989; Lowry et al . , 1989; Clarke et al . , 1988) . The farnesylated p21 ras binds loosely to the plasma membrane, from which most of it can be released with salt (Hancock, et al . , 1989) . After binding to the membrane, some P21 ras proteins are further modified by the addition of palmitate in thioester linkage to cysteines near the farnesylated C- terminal cysteine (Hancock et al . , 1989) . Palmitylation renders the protein even more hydrophobic and anchors it more tightly to the plasma membrane. However, although it appears to be clear that farnesylation is a key event in ras-related cancer development, prior to now, the nature of this event has remained obscure. Nothing has been known previously, for example, of the nature of the enzyme or enzymes which may be involved in ras tumorigenesis or required by the tumor cell to achieve farnesylation. If the mechanisms that underlie farnesylation of cancer-related proteins such as P21 ras could be elucidated, then procedures and perhaps even pharmacologic agents could be developed in an attempt to control or inhibit expression of the oncogenic phenotype in a wide variety of cancers. It goes without saying that such discoveries would be of pioneering proportions in cancer therapy. SUMMARY OF THE INVENTION The present invention addresses one or more short¬ comings in the prior art through the identification and characterization of an enzyme, termed farnesyl:protein transferase or CAAX farnesyltransferase, involved in the oncogenic process through the transfer of farnesyl groups to various proteins including oncogenic ras proteins. The invention relates particularly to the molecular cloning of mammalian farnesyl:protein transferase subunits, to the purification of the native or recombinant enzyme, to protein and peptide substances that are capable of inhibiting the enzyme, and to assay methods for the identification of further inhibitory compounds. A certain object of the present invention is therefore to provide ready means for obtaining farnesyl transferase enzymes, by purification of the native enzyme from tissues of choice, or by purification of the recombinant enzyme from host cells that express the constituent subunits, which methods are proposed to be generally applicable to the purification of all such farnesyl protein transferases. It is an additional object of the invention to provide means for obtaining these enzymes in a relatively purified form, allowing their use in predictive assays for identifying compounds having the ability to reduce the activity of or inhibit the farnesyl transferase activity, particularly in the context of p21 ras proteins. It is a still further object of the invention to identify classes of compounds which demonstrate farnesyl transferase inhibiting activity, along with a potential application of these compounds in the treatment of cancer, particularly ras-related cancers. Farnesyl:Protein Transferase Characterization Accordingly, in certain embodiments, the present invention relates to compositions which include a purified farnesyl protein transferase (CAAX farnesyltransferase) enzyme, characterized as follows: a) capable of catalyzing the transfer of farnesyl to a protein or peptide having a farnesyl acceptor moiety; b) capable of binding to an affinity chromatography medium comprised of TKCVIM (seq id no:9) coupled to a suitable matrix; c) exhibiting a molecular weight of between about 70,000 and about 100,000 upon gel filtration chromatography; and d) having a farnesyl transferase activity that is capable of being inhibited by one of the following peptides : i) TKCVIM (seq id no:9) ; ii) CVIM (seq id no:10); or iii) KKSKTKCVIM (seq id no:ll) . As used herein, the phrase "capable of catalyzing the transfer of farnesol to a protein or peptide having a farnesyl acceptor moiety, " is intended to refer to the functional attributes of farnesyl transferase enzymes of the present invention, which catalyze the transfer of farnesol, typically in the form of all- rans farnesol, from all- rans farnesyl pyrophosphate to proteins which have a sequence recognized by the enzyme for attachment of the farnesyl moieties. Thus, the term "farnesyl acceptor moiety" is intended to refer to any sequence, typically a short amino acid recognition sequence, which is recognized by the enzyme and to which a farnesyl group will be attached by such an enzyme. Farnesyl acceptor moieties have been characterized by others in various proteins as a four amino acid sequence found at the carboxy terminus of target proteins. This four amino acid sequence has been characterized as -C-A-A-X (seq id no:12) , wherein "C" is a cysteine residue, "A" refers to any aliphatic amino acid, and "X" refers to any amino acid. Of course, the term "aliphatic amino acid" is well-known in the art to mean any amino acid having an aliphatic side chain, such as, for example, leucine, isoleucine, alanine, methionine, valine, etc. While the most preferred aliphatic amino acids, for the purposes of the present invention include valine and isoleucine, it is believed that virtually any aliphatic amino acids in the designated position can be recognized within the farnesyl acceptor moiety. In addition, the enzyme has been shown to recognize a peptide containing a hydroxylated amino acid (serine) in place of an aliphatic amino acid (CSIM; seq id no:13) . Of course, principal examples of proteins or peptides having a farnesyl acceptor moiety, for the purposes of the present invention, will be the p21 ras proteins, including p21 H"ras , p21 K"rasA , p21 κ'rasB and p21 N"ras . Thus, in light of the present disclosure, a wide variety of peptidyl sequences having a farnesyl acceptor moiety will become apparent. As outlined above, the inventors have discovered that the farnesyl transferase enzyme is capable of binding to an affinity chromatography medium comprised of the peptide TKCVIM (seq id no:9) , coupled to a suitable matrix. This feature of the farnesyl transferase enzyme was discovered by the present inventors in developing techniques for its isolation. Surprisingly, it has been found that the coupling of a peptide such as one which includes CVIM (seq id no:10), as does TKCVIM (seq id no:9) , to a suitable chromatography matrix allows for the purification of the protein to a significant degree, presumably through interaction and binding of the enzyme to the peptidyl sequence. A basis for this interaction could be posited as due to the apparent presence of a farnesyl acceptor moiety within this peptide. The phrase "capable of binding to an affinity chromatography medium comprised of TKCVIM coupled to a suitable matrix, " is intended to refer to the ability of the protein to bind to such a medium under conditions as specified herein below. There will, of course, be conditions, such as when the pH is below 6.0, wherein the farnesyl transferase enzyme will not bind effectively to such a matrix. However, through practice of the techniques disclosed herein, one will be enabled to achieve this important objective. There are numerous chromatography matrixes which are known in the art that can be applied to the practice of this invention. The inventors prefer to use activated CH-Sepharose 4B, to which peptides such as TKCVIM (seq id no:9) , or which incorporate the CVIM (seq id no:10) structure, can be readily attached and washed with little difficulty. However, the present invention is by no means limited to the use of CH-Sepharose 4B, and includes within its intended scope the use of any suitable matrix for performing affinity chromatography known in the ar . Examples include solid matrices with covalently bound linkers, and the like, as well as matrices that contain covalently associated avidin, which can be used to bind peptides that contain biotin. Farnesyl transferase enzymes of the present invention have typically been found to exhibit a molecular weight of between about 70,000 and about 100,000 upon gel filtration chromatography. For comparison purposes, this molecular weight was identified for farnesyl protein transferase through the use of a Superose 12 column, using a column size, sample, load and parameters as described herein below. It is quite possible, depending on the conditions employed, that different chromatographic techniques may demonstrate a farnesyl transferase protein that has an apparent molecular weight somewhat different than that identified using the preferred techniques set forth in the examples. It is intended therefore, that the molec¬ ular weight determination and range identified for farnesyl transferase in the examples which follow, are designated only with respect to the precise techniques disclosed herein. It has been determined that the farnesyl:protein transferase can be characterized as including two subunits, each having a molecular weight of about 45 to 50 kDa, as estimated by SDS polyacrylamide gel electrophoresis (SDS/PAGE) . These subunits have been designated as and β , with the α. subunit migrating slightly higher than the β subunit, which suggests that the subunit may be slightly larger. From tryptic peptide sequence analyses and molecular cloning the nature of the and β subunits as distinct proteins, encoded by separate genes, has been confirmed. Peptide sequences obtained from the rat brain subunits were subsequently found to be consistent with the amino acid sequences predicted by the DNA coding sequences: TABLE I RAT FARNESYL;PROTEIN TRANSFERASE PEPTIDE SEQUENCES subunit : * 1) * R A E W A D I D P V P Q N D G P S P V V Q I I Y S K D E 2) D A I E L N A A N Y T V W H F R * * * 3) H F V I S N T T G Y S D H R R R A V 4) V L V E W L K 5) L V P H N E S A W N Y L K * * 6) L W D N E L Q Y V D Q L L K β subunit : 7) * A Y C A A S V A S L T N I I T P D L F E G V K E S T A 8) * L L Q W V T S R G S Q 9) * I Q A T T H F L Q K P V P G F E E C E D A V T * D P V S 10) I Q E V F S S Y K 11) F E G G F Q G R 12) F N H L V P_P R P The sequences shown in Table I were obtained from HPLC-purified tryptic peptides isolated from the - or β- subunit of purified rat farnesyltransferase (Reiss et al . , 1991) . Each peptide represents a pure species from a single HPLC peak. Asterisks denote ambiguous residues from amino acid sequencing. The amino acid sequences of all 6 peptides of each subunit (shown above) are found within continuous segments of the amino acid sequence predicted from the respective cDNA clones (seq id no:l; seq id no:3) , except for the differences indicated below certain of the peptide sequences. The inventors have found that the holoenzyme forms a stable complex with all- rans [ 3 H] farnesyl pyrophosphate (FPP) that can be isolated by gel electrophoresis. The [ 3 H]FFP is not covalently bound to the enzyme, and is released unaltered when the enzyme is denatured. When incubated with an acceptor such as p21 H " ras , the complex transfers [ 3 H] farnesyl from the bound [ 3 H]FFP to the ras protein. Furthermore, crosslinking studies have shown that p21 H"ras binds to the β subunit, raising the possibility that the [ 3 H]FFP binds to the α subunit. If this is the case, it would invoke a reaction mechanism in which the α subunit act as a prenyl pyrophosphate carrier that delivers FPP to p21 H " ras , which is bound to the β subunit. Interestingly, the inventors have recently discovered that the α subunit is shared with another prenyltransferase, geranylgeranyltransferase, that attaches 20-carbon geranylgeranyl to Ras-related proteins. An additional property discovered for farnesyl transferase enzymes is that they can be inhibited by peptides or proteins, particularly short peptides, which include certain structural features, related in some degree to the farnesyl acceptor moiety discussed above. As used herein, the word "inhibited" refers to any degree of inhibition and is not limited for these purposes to only total inhibition. Thus, any degree of partial inhibition or relative reduction in farnesyl transferase activity is intended to be included within the scope of the term "inhibited." Inhibition in this context includes the phenomenon by which a chemical constitutes an alternate substrate for the enzyme, and is therefore farnesylated in preference to the ras protein, as well as inhibition where the compound does not act as an alternate substrate for the enzyme. Preparation of Farnesyl:Protein Transferase The present invention is also concerned with tech¬ niques for the identification and isolation of farnesyl transferase enzymes, and particularly mammalian farnesyl transferases (CAAX farnesyltransferases) . Techniques are herein disclosed for the isolation of farnesyl transferase which are believed to be applicable to the purification of the native protein, or alternatively, to the purification of the recombinant enzyme following the molecular cloning and co-expression of the constituent subunits. " An important feature of the purification scheme disclosed herein involves the use of short peptide sequences which the inventors have discovered will bind the enzyme, allowing their attachment to chromatography matrices, such matrices may, in turn, be used in connection with affinity chromatography to purify the enzyme to a relative degree. Thus, in certain embodiments, the present invention is concerned with a method of preparing a farnesyl transferase enzyme which includes the steps of: (a) preparing a cellular extract which includes the enzyme; (b) subjecting the extract to affinity chromatography on an affinity chromatography medium to bind the enzyme thereto, the medium comprised of a farnesyl transferase binding peptide coupled to a suitable matrix; (c) washing the medium to remove impurities; and (d) eluting the enzyme from the washed medium. Thus, the first step of the purification protocol involves simply preparing a cellular extract which includes the enzyme. The inventors have discovered that the enzyme is soluble in buffers such as low-salt buffers, and it is proposed that virtually any buffer of this type can be employed for initial extraction of the protein from the tissue of choice or from recombinant cells in which the constituent subunits of the enzyme are expressed. The inventors prefer a 50mM Tris-chloride, pH 7.5, buffer which includes a divalent chelator (e.g., ImM EDTA, ImM EGTA) , as well as protease inhibitors such as phenylmethylsulphonyl fluoride (PMSF) and/or leupeptin. Of course, those of skill in the art will recognize that a variety of other types of buffers may be employed as extractants where desired, so long as the enzyme is extractable in such a buffer and its subsequent activity is not adversely affected to a significant degree. In embodiments concerning the purification of the native enzyme, the choice of tissue from which one will seek to obtain the farnesyl transferase enzyme is not believed to be of crucial importance. In fact, it is believed that farnesyl transferases are components of virtually all living cells. Therefore, the tissue of choice will typically be that which is most readily available to the practitioner. In that farnesyl transferase action appears to proceed similarly in most systems studied, including, cultured hamster cells, rat brain, and even yeast, it is believed that this enzyme will exhibit similar qualities, regardless of its source of isolation. In preferred embodiments, the inventors have isolated the native enzyme from rat brains in that this source is readily available. However, numerous other sources are contemplated to be directly applicable for isolation of the native enzyme, especially mammalian tissues such as liver, and human placenta, and also reticulocytes, or even yeast. Those of skill in the art, in light of the present disclosure, should appreciate that the techniques disclosed herein will be generally applicable to all such farnesyl transferases. It will also be appreciated that the enzyme may be purified from recombinant cells prepared in accordance with the present invention. The techniques disclosed for the isolation of native farnesyl transferase are believed to be equally applicable to the purification of the protein from recombinant host cells, whether bacterial or eukaryotic, in which DNA segments encoding the selected constituent subunit has been expressed or co-expressed. After the cell extract is prepared the enzyme is preferably subjected to two partial purification steps prior to affinity chromatography. These steps comprise preliminary treatment with 30% saturated ammonium sulfate which removes certain contaminants by precipitation. This is followed by treatment with 50% saturated ammonium sulfate, which precipitates the farnesyl transferase. The pelleted enzyme is then dissolved in a suitable buffer, such as 20mM Tris-chloride (pH 7.5) containing ImM DTT and 20μM ZnCl 2 , dialyzed against the same buffer, and then subjected to further purification steps. In preferred embodiments, the dialyzed solution containing the enzyme is applied to a column containing an ion exchange resin such as Mono Q. After washing of the column to remove contaminants, the enzyme is eluted with a gradient of 0.25 - 1.0M NaCl in the same buffer. The enzyme activity in each fraction is assayed as described below, and the fractions containing active enzyme are pooled and applied to the affinity column described below. It is, of course, recognized that the preliminary purification steps described above are preferred laboratory procedures that might readily be replaced with other procedures of equivalent effect such as ion exchange chromatography on other resins or gel filtration chromatography. Indeed, it is possible that these steps could even be omitted and the crude cell extract might be carried directly to affinity chromatography. After the preliminary purification steps, the extract may be subjected to affinity chromatography on an affinity chromatography medium which includes a farnesyl transferase binding peptide coupled to a suitable matrix. Typically, preferred farnesyl transferase binding peptides will comprise a peptide of at least 4 amino acids in length and will include a carboxy terminal sequence of -C-Aj-Aj-X, wherein: C = cysteine; Ai = any amino acid (aliphatic, aromatic, or hydroxy) ; A 2 = an aliphatic amino acid, preferrably leucine, isoleucine or valine; and X = preferably methionine or serine, less preferably glutamine or cysteine, and even less preferably any other amino acid other than leucine. Preferred binding peptides of the present invention which fall within the above general formula include structures such as -C-V-I-M (seq id no:10), -C-S-I-M (seq id no:13) and -C-A-I-M (seq id no:14), all of which structures are found to naturally occur in proteins which are believed to be acted upon by farnesyl protein transferases in nature. Particularly preferred are relatively short peptides, such as on the order of about 4 to about 10 amino acids in length which incorporate one of the foregoing binding sequences. Of particular preference is the peptide T-K-C-V-I-M (seq id no:9), which has been effectively employed by the inventors in the isolation of farnesyl protein transferase. The next step in the overall general purification scheme involves simply washing the medium to remove impurities. That is, after subjecting the extract to affinity chromatography on the affinity matrix, one will desire to wash the matrix in a manner that will remove the impurities while leaving the farnesyl transferase enzyme relatively intact on the medium. A variety of techniques are known in the art for washing matrices such as the one employed herein, and all such washing techniques are intended to be included within the scope of this invention. Of course, for washing purposes, one will not desire to employ buffers that will release or otherwise alter or denature the enzyme. Thus, one will typically want to employ buffers which contain non- denaturing detergents such as octylglucoside buffers, but will want to avoid buffers containing, e.g., chaotropic reagents which serve to denature proteins, as well as buffers of low pH (e.g., less than 7), or of high ionic strength (e.g., greater than 1.0M), as these buffers tend to elute the bound enzyme from the affinity matrix. After the matrix-bound enzyme has been sufficiently washed, for example in a medium-ionic strength buffer at essentially neutral pH, the specifically bound material can be eluted from the column by using a similar buffer but of reduced pH (for example, a pH of between about 4 and 5.5) . At this pH, the enzyme will typically be found to elute from the preferred affinity matrices disclosed in more detail hereinbelow. While it is believed that advantages in accordance with the invention can be realized simply through affinity chromatography techniques, additional benefits will be achieved through the application of additional purification techniques, such as gel filtration techniques. For example, the inventors have discovered that Sephacryl S-200 high resolution gel columns can be employed with significant benefit in terms of protein purification. However, the present disclosure is by no means limited to the use of Sephacryl S-200, and it is believed that virtually any type of gel filtration arrangement can be employed with some degree of benefit. For example, one may wish to use techniques such as gel filtration, employing media such as Superose, Agarose, or even Sephadex. Through the application of various of the foregoing approaches, the inventors have successfully achieved farnesyl transferase enzyme compositions of relatively high specific activity, measured in terms of ability to transfer farnesol from all- trans farnesyl pyrophosphate. For the purposes of the present invention, one unit of activity is defined as the amount of enzyme that transfers lpmol of farnesol from all- trans farnesyl pyrophosphate (FPP) into acid-precipitable p21 H ' ras per hour under the conditions set forth in the Examples. Thus, in preferred embodiments the present invention is concerned with compositions of farnesyl transferase which include a specific activity of between about 5 and about 10 units/mg of protein. In more preferred embodiments, the present invention is concerned with compositions which exhibit a farnesyl transferase specific activity of between about 500 and about 600,000 units/mg of protein. Thus, in terms of the unit definition set forth above, the inventors have been able to achieve compositions having a specific activity of up to about 600,000 units/mg using techniques disclosed herein. Cloning of Farnesyl:Protein Transferase Subunits Important aspects of the present invention concern isolated DNA segments and recombinant vectors encoding the a and β subunits of mammalian farnesyl:protein transferases (CAAX farnesyltransferases) , and the creation of recombinant host cells through the application of DNA technology, which express one, or preferably both, of these polypeptides. As used herein, the term "DNA segment" in intended to refer to a DNA molecule which has been isolated free of total genomic DNA of a particular species. Therefore, a DNA segment encoding a subunit of farnesyl:protein transferase is intended to refer to a DNA segment which contains such coding sequences yet is isolated away from total genomic DNA of the species from which the DNA is obtained. Included within the term "DNA segment", are DNA segments which may be employed in the preparation of vectors, as well as the vectors themselves, including, for example, piasmids, cosmids, phage, viruses, and the like. In particular embodiments, the invention concerns isolated DNA segments and recombinant vectors incorporating DNA sequences which encode a farnesyl:protein transferase subunit that includes within its amino acid sequence the amino acid sequence of seq id no:l or seq id no:3, corresponding to rat brain farnesyl transferase subunits a and β, respectively. Moreover, in other particular embodiments, the invention concerns isolated DNA segments and recombinant vectors incorporating DNA sequences which encode a farnesyl:protein transferase subunit that includes within its amino acid sequence the amino acid sequence of seq id no:5 or seq id no:7, corresponding to human farnesyl transferase subunits and β, respectively. Recombinant vectors and isolated segments may therefore variously include the α or β subunit coding regions themselves, coding regions bearing selected alterations or modifications in the basic coding region or may encode larger polypeptides which nevertheless include sequences which will confer farnesyl transferase activity when said polypeptide is combined with the alternate subunit. However, it will be understood that this aspect of the invention is not limited to the particular nucleic acid and amino acid sequences of seq id no:l and no:2 and seq id no:5 and no:6 (a subunit) or seq id no:3 and no: and seq id no:7 and 8 (β subunit) . Accordingly, DNA segments prepared in accordance with the present invention may also encode biologically functional equivalent proteins or peptides which have variant amino acids sequences. Such sequences may arise as a consequence of codon redundancy and functional equivalency which are known to occur naturally within nucleic acid sequences and the proteins thus encoded. Alternatively, functionally equivalent proteins or peptides may be created via the application of recombinant DNA technology, in which changes in the protein structure may be engineered, based on considerations of the properties of the amino acids being exchanged. The recombinant cloning of cDNAs encoding the farnesyl transferase cn and β subunits was achieved through the use of the peptide sequence information set forth above which was used in the preparation of subunit- specific oligonucleo-tides. Such oligonucleotides could be employed in the direct hybridization screening of a clone bank. However, the inventors preferred to use the peptide sequences in the preparation of primers for use in PCR amplification and partial sequencing of the selected subunit gene to confirm the underlying DNA sequence, and to prepare longer and more specific probes for use in clone bank screening. In screening for the farnesyl transferase subunit- specific sequences, the inventors chose to use a cDNA clone bank prepared from poly A + RNA. However, it iε believed that the type of clone bank used is not crucial and that, if desired, one could employ a genomic clone bank. Similarly, in that the farnesyl transferase enzyme appears to be fairly ubiquitous in nature, it is believed that virtually any eukaryotic cell source may be employed for the preparation of RNA from which the clone bank is to be generated. One may mention by way of example, yeast, mammalian, plant, eukaryotic parasites and even viral-infected types of cells as the source of starting poly A + RNA. As the protein was initially purified from a mammalian source (rat) , it is contemplated that particular advantages may be found in the use of mammalian cells, such as rat or human cell lines, as an RNA source. One may, of course, wish to first test such a cell line to ensure that relatively high levels of the farnesyl transferase enzyme are being produced by the selected cells. Rat brain, PC12 (a rat adrenal tumor cell line) and KNRK (a newborn rat kidney cell line) were preferred by the present inventors as they exhibited high levels of endogenous farnesyl :protein transferase activity. The type of cDNA clone bank used in the screening procedure is not believed to be particularly critical . However, one will likely find particular benefit through the preparation and use of a phage-based bank, such as λgtlO or λgtll, preferably using a particle packaging system. Phage-based cDNA banks are preferred because of the large numbers of recombinants that may be prepared and screened will relative ease. The manner in which the cDNA itself is prepared is again not believed to be particularly crucial. However, the inventors successfully employed both oligo dT and randomly primed cDNA, from a consideration of the difficulties which may arise in the reverse transcription of a large mRNA molecule. Once a clone bank has been prepared, it may be screened in a number of fashions. For example, as mentioned above, one could employ the subunit peptide sequences set forth above for the preparation of nucleotide probes with which to directly screen the clone bank. A more preferable approach was found to be to use such sequences in the preparation of primers which may were used in PCR-based reactions to amplify and then sequence portions of the selected subunit gene, to thereby confirm the actual underlying DNA sequence, and to prepare longer and more specific probes for further screening. These primers may also be employed for the preparation of cDNA clone banks which are enriched for 3 ' and/or 5' sequences. This may be important, e.g., where less than a full length clone is obtained through the initially prepared bank. If a less than full length clone was obtained on initial screening, the entire sequence could be subsequently obtained through the application of 5' and/or 3' extension technology, as required. The techniques for obtaining an extended farnesyl transferase subunit clone will be known to those of skill in the art in light of the present disclosure. The procedures used are those described in Frohman et al . (1988) , involving a combination of reverse transcription, tailing with terminal deoxytransferase and, finally, PCR. It is proposed that the DNA segments of the present invention may be employed for a variety of applications. For example, a particularly useful application concerns the recombinant production of the individual subunits or proteins or peptides whose structure is derived from that of the subunits, or in the recombinant production of the holoenzyme following co-expression of the two subunits. Additionally, the farnesyl transferase-encoding DNA segments of the present invention can also be used in the preparation of nucleic acid probes or primers, which can, for example, be used in the identification and cloning of farnesyl transferase genes or related genomic sequences, or in the study of subunit (s) expression, and the like. Expression of Farnesyl:Protein Transferase Subunits Turning firstly to the expression of the cloned subunits. Once a suitable (full length if desired) clone or clones have been obtained, whether they be cDNA based or genomic, one may proceed to prepare an expression system for the recombinant preparation of one, or preferably both, of the subunits. The engineering of DNA segment (s) for expression in a prokaryotic or eukaryotic system may be performed by techniques generally known to those of skill in recombinant expression. It is believed that virtually any expression system may be employed in the expression of either or both subunits. Both subunits of the enzyme have been successfully co-expressed in eukaryotic expression systems with the production of active enzyme, but it is envisioned that bacterial expression systems may ultimately be preferred for the preparation of farnesyl transferase for all purposes. The cDNAs for both subunits have been separately expressed in bacterial systems, with the encoded proteins being expressed as fusions with Schistosoma japonicum glutathione S-transferase. It is believed that bacterial expression will ultimately have numerous advantages over eukaryotic expression in terms of ease of use and quantity of materials obtained thereby. Furthermore, it is proposed that co-transformation of host cells with DNA segments encoding both the c and β subunits will provide a convenient means for obtaining active enzyme. However, separate expression followed by reconstitution is also certainly within the scope of the invention. Both cDNA and genomic sequences are suitable for eukaryotic expression, as the host cell will, of course, process the genomic transcripts to yield functional mRNA for translation into protein. It is similarly believed that almost any eukaryotic expression system may be utilized for the expression of either, or preferably, both of the farnesyl transferase subunits, e.g., baculovirus-based, glutamine synthase- based or dihydrofolate reductase-based systems could be employed. However, in preferred embodiments, it is contemplated that plasmid vectors incorporating an origin of replication and an efficient eukaryotic promoter, as exemplified by the eukaryotic vectors of the pCMV series, such as pCMV5, will be of most use. For expression in this manner, one would position the coding sequences adjacent to and under the control of the promoter. It is understood in the art that to bring a coding sequence under the control of such a promoter, one positions the 5' end of the transcription initiation site of the transcriptional reading frame of the protein between about 1 and about 50 nucleotides "downstream" of (i.e., 3' of) the chosen promoter. Where eukaryotic expression is contemplated, one will also typically desire to incorporate into the transcriptional unit which includes the enzyme, an appropriate polyadenylation site (e.g., 5' -AATAAA-3' ) if one was not contained within the original cloned segment . Typically, the poly A addition site is placed about 30 to 2000 nucleotides "downstream" of the termination site of the protein at a position prior to transcription termination. As noted above, it is proposed that in embodiments concerning the production of farnesyl transferase enzyme, the a and β subunits may be co-expressed in the same cell. This may be achieved by co-transfecting the cell with two distinct recombinant vectors, each bearing a copy of either the α.- or β-encoding DNA. Alternatively, a single recombinant vector may be constructed to include the coding regions for both of the subunits, which could then be expressed in cells transfected with the single vector. In either event, the term "co-expression" herein refers to the expression of both the cn and β subunits of farnesyl transferase in the same recombinant cell. It is contemplated that virtually any of the commonly employed host cells can be used in connection with the expression of one, or preferably both, of the farnesyl transferase subunits in accordance herewith. Examples include cell lines typically employed for eukaryotic expression such as 239, AtT-20, HepG2, VERO, HeLa, CHO, WI 38, BHK, COS-7, RIN and MDCK cell lines. A preferred line for use in eukaryotic expression embodiments of the present invention has been found to be the human embryonic kidney cell line, 293. In accordance with the general guidelines described above, a preferred method for expressing farnesyl transferase DNA has been found to be the transfection of human embryonic kidney 293 cells with expression vectors termed pFT-α or pFT-β. The pFT expression vectors are constructed from pCMV5, a plasmid that contains the promoter-enhancer region of the major immediate early gene of human cytomegalovirus (Andersson et al . , 1989) . Nucleic Acid Hybridization The DNA sequences disclosed herein will also find utility as probes or primers in nucleic acid hybridization embodiments. As ■ such, it is contemplated that oligonucleotide fragments corresponding to the sequences of seq id no:2, seq id no:4, seq id no:6 and seq id no:8 for stretches of between about 10 nucleotides to about 30 nucleotides will find particular utility, with even longer sequences, e.g., 40, 50, 60, even up to full length, being even more particularly preferred. The ability of such nucleic acid probes to specifically hybridize to farnesyl transferase subunit-encoding sequences will enable them to be of use in a variety of embodiments. Most importantly, the probes can be used in a variety of assays for detecting the presence of complementary sequences in a given sample. However, other uses are envisioned, including the use of the sequence information for the preparation of mutant species primers, or primers for use in preparing other genetic constructions. The use of a hybridization probe of about 10 nucleotides in length allows the formation of a duplex molecule that is both stable and selective. Molecules having complementary sequences over stretches greater than 10 bases in length are generally preferred, though, in order to increase stability and selectivity of the hybrid, and thereby improve the quality and degree of specific hybrid molecules obtained. One will generally prefer to design nucleic acid molecules having gene- complementary stretches of 15 to 20 nucleotides, or even longer where desired. Such fragments may be readily prepared by, for example, directly synthesizing the fragment by chemical means, by application of nucleic acid reproduction technology, such as the PCR technology of U.S. Patent 4,603,102 (herein incorporated by reference) or by introducing selected sequences into recombinant vectors for recombinant production. Accordingly, the nucleotide sequences of the invention may be used for their ability to selectively form duplex molecules with complementary stretches of farnesyl transferase genes or cDNAs. Depending on the application envisioned, one will desire to employ varying conditions of hybridization to achieve varying degrees of selectivity of probe towards target sequence. For applications requiring high selectivity, one will typically desire to employ relatively stringent conditions to form the hybrids, e.g., one will select relatively low salt and\or high temperature conditions, such as provided by 0.02M-0.15M NaCl at temperatures of 50°C to 70°C. Such selective conditions tolerate little, if any, mismatch between the probe and the template or target strand, and would be particularly suitable for isolating farnesyl transferase genes. Of course, for some applications, for example, where one desires to prepare mutants employing a mutant primer strand hybridized to an underlying template or where one seeks to isolate farnesyl transferase-encoding sequences for related species, functional equivalents, or the like, less stringent hybridization conditions will typically be needed in order to allow formation of the heteroduplex. In these circumstances, one may desire to employ conditions such as 0.15M-0.9M salt, at temperatures ranging from 20°C to 55°C. Cross-hybridizing species can thereby be readily identified as positively hybridizing type of target nucleic acid, source of nucleic acid, size of hybridization probe, etc.) . Following washing of the hybridized surface so as to remove nonspecifically bound probe molecules, specific hybridization is detected, or even quantified, by means of the label. Biological Functional Equivalent Amino Acids As mentioned above, modification and changes may be made in the structure of the farnesyl transferase (CAAX farnesyltransferase) subunits and still obtain a molecule having like or otherwise desirable characteristics. For example, certain amino acids may be substituted for other amino acids in a protein structure without appreciable loss of interactive binding capacity with structures such as, for example, antigen-binding regions of antibodies or binding sites on substrate molecules. Since it is the interactive capacity and nature of a protein that defines that protein's biological functional activity, certain amino acid sequence substitutions can be made in a protein sequence (or, of course, its underlying DNA coding sequence) and nevertheless obtain a protein with like or even counterveiling properties (e.g., antagonistic v. agonistic) . It is thus contemplated by the inventors that various changes may be made in the sequence of the peptides (or underlying DNA) without appreciable loss of their biological utility or activity. In making such changes, the hydropathic index of amino acids may be considered. The importance of the hydropathic amino acid index in conferring interactive biologic function on a protein is generally understood in the art (Kyte & Doolittle, 1982) . It is known that certain amino acids may be substituted for other amino acids having a similar hydropathic index or score and still result in a protein with similar biological activity. Each amino acid has been assigned a hydropathic index on the basis of their hydrophobicity and charge characteristics, these are: isoleucine (+4.5) ; valine (+4.2) ; leucine (+3.8); phenylalanine (+2.8) ; cysteine/cystine (+2.5) ; methionine (+1.9) ; alanine (+1.8) ; glycine (-0.4); threonine (-0.7) ; serine (-0.8) ; tryptophan (-0.9) ; tyrosine (-1.3) ; proline (-1.6) ; histidine (-3.2) ; glutamate (-3.5) ; glutamine (-3.5) ; aspartate (-3.5); asparagine (-3.5) ; lysine (-3.9) ; and arginine (-4.5) . As the relative hydropathic character of the amino acids determines the secondary structure of the resultant protein, which in turn defines the interaction of the protein with other molecules, for example, enzymes, substrates, inhibitors, receptors, antibodies, antigens, and the like. It is known in the art that an amino acid may be substituted by another amino acid having a similar hydropathic index and still obtain a biological functionally equivalent protein. In such changes, the substitution of amino acids whose hydropathic indices are within +2 is preferred, those which are within +1 are particularly preferred, and those within +0.5 are even more particularly preferred. Substitution of like amino acids can also be made on the basis of hydrophilicity, particularly where the biological functional equivalent protein or peptide thereby created is intended for use in immunological embodiments. U.S. Patent 4,554,101, incorporated herein by reference, states that the greatest local average hydrophilicity of a protein, as governed by the hydrophilicity of its adjacent amino acids, correlates with its immunogenicity and antigenicity, i.e. with a biological property of the protein. As detailed in U.S. Patent 4,554,101, the following hydrophilicity values have been assigned to amino acid Inhibitors of Farnesyl:Protein Transferase Of principal importance to the present invention is the discovery that proteins or peptides which incorporate a farnesyl acceptor sequence, such as one of the farnesyl acceptor sequences discussed above, function as inhibitors of farnesyl :protein transferase, and therefore may serve as a basis for anticancer therapy. In particular, it has been found that farnesyl acceptor peptides can successfully function both as false substrates that serve to inhibit the farnesylation of natural substrates such as p21 ras , and as direct inhibitors which are not themselves farnesylated. Compounds falling into the latter category are particularly important in that these compounds are "pure" inhibitors that are not consumed by the inhibition reaction and can continue to function as inhibitors. Both types of compounds constitute an extremely important aspect of the invention in that they provide a means for blocking farnesylation of p21 ras proteins, for example, in an affected cell system. The farnesyl transferase inhibitor embodiments of the present invention concern in a broad sense a peptide or protein other than p21 ras proteins, lamin a or lamin b, or yeast mating factor a, which peptide or protein includes a farnesyl acceptor sequence within its structure and is further capable of inhibiting the farnesylation of p21 ras by farnesyl transferase. In preferred embodiments, the farnesyl transferase inhibitor of the present invention will include a farnesyl acceptor or inhibitory amino acid sequence having the amino acids -C-A,-A 2 -X, wherein: C = cysteine; A, = any amino acid (aliphatic, aromatic, or hydroxy) ; A 2 = an aliphatic amino acid, preferrably leucine, isoleucine or valine; and X preferably methionine or serine, less preferably glutamine or cysteine, and even less preferably any other amino acid other than leucine. Whereas it was previously proposed that the X amino acid could be any amino acid, it has more recently been discovered that one will prefer that x will be methionine or serine, less preferably glutamine or cysteine, and even less preferably any other amino acid other than leucine. Leucine should not be employed as the carboxy terminal amino acid in that such peptides are known to be substrates for a separate enzyme, geronylgeronyl- transferase (Seabra et al . , 1991) . Typically, the farnesyl acceptor or inhibitory amino acid sequence will be positioned at the carboxy terminus of the protein or peptide such that the cysteine residue is in the fourth position from the carboxy terminus. In preferred embodiments, the inhibitor will be a relatively short peptide such as a peptide from about 4 to about 10 amino acids in length. To date, the most preferred inhibitor tested is a tetrapeptide which incorporates the above-mentioned C-A-A-X (seq id no:12) recognition structure. It is possible that even shorter peptides will ultimately be preferred for practice of the invention in that the shorter the peptide, the greater the uptake by such peptide by biological systems, and the reduced likelihood that such a peptide will be destroyed or otherwise rendered biologically ineffective prior to effecting inhibition. However, numerous suitable inhibitory peptides have been prepared and tested by the present inventors, and shown to inhibit enzymatic activities virtually completely, at reasonable concentrations, e.g., between about 1 and 3μM (with 50% inhibitions on the order of 0.1 to 0.5μM) . While, broadly speaking, it is believed that compounds exhibiting an IC 50 of between about O.OlμM and lOμM will have some utility as farnesyl transferase inhibitors, the more preferred compounds will exhibit an IC 50 of between O.OlμM and lμM. The most preferred compounds will generally have an IC 50 of between about O.OlμM and 0.3μM. Exemplary peptides which have been prepared, tested and shown to inhibit farnesyl transferase at an . IC 50 of between 0.01 and lOμM include CVIM (seq id no:10); KKSKTKCVIM (seq id no:11); TKCVIM (seq id no:9); RASNRSCAIM (seq id no:15) ; TQSPQNCSIM (seq id no:16) ; and the following tetrapeptides: CUM (seq id no:17) ; CWM (seq id no:18) ; CVLS (seq id no:19) ; CVLM (seq id no:20); CAIM (seq id no:14) ; CSIM (seq id no:13) ; CCVQ (seq id no:21) ; CIIC (seq id no:22) ; CIIS (seq id no:23) ; CVIS (seq id no:24) ; CVIA (seq id no:25); CVIL (seq id no:26); CLIL (seq id no:27); CLLL (seq id no:28) ; CTVA (seq id no:29) ; CVAM (seq id no:30) ; CKIM (seq id no:31); CLIM (seq id no:32); CFIM (seq id no:33); CVFM (seq id no:34) ; CVIF (seq id no:35) ; CEIM (seq id no:36) ; CGIM (seq id no:37) ; CPIM (seq id no:38) ; CVYM (seq id no:39) ; CVTM (seq id no:40) ; CVPM (seq id no:41) ; CVSM (seq id no:42) ; CVIV (seq id no:43) ; CVIP (seq id no:44) ; CVII (seq id no:45) ; CVWM (seq id no:46) ; CIFM (seq id no:47) . A variety of peptides have been synthesized and tested such that now the inventors can point out peptide sequences having particularly high inhibitory activity, i.e., wherein relatively lower concentrations of the peptides will exhibit an equivalent inhibitory activity (IC 50 ) . Interestingly, it has been found that slight changes in the sequence of the acceptor site can result in loss of inhibitory activity. Thus, when TKCVIM is changed to TKVCIM, the inhibitory activity of the peptide is reversed. Similarly, when a glycine is substituted for one of the aliphatic amino acids in CAAX, a decrease in inhibitory activity is observed. However, it is proposed that as long as the general formula as discussed above is observed, one will achieve a structure that is inhibitory to farnesyl transferase. A particularly important discovery is the finding that the incorporation of an aromatic residue such as phenylalanine, tyrosine or tryptophan into the third position of the CAAX (seq id no:12) sequence will result in a "pure" inhibitor. As used herein, a "pure" farnesyl :protein transferase inhibitor is intended to refer to one which does not in itself act as a substrate for farnesylation by the enzyme. This is particularly important in that the inhibitor is not consumed by the inhibition process, leaving the inhibitor to continue its inhibitory function unabated. Exemplary compounds which have been tested and found to act as pure inhibitors include CVFM (seq id no:34) , CVWM (seq id no:46) , CVYM (seq id no:39) , CIFM (seq id no:47) , CV(pCl-F)M, L- penicillamine-VFM, and L-penicillamine-VIM. Pure inhibitors will therefore incorporate an inhibitory amino acid sequence rather than an acceptor sequence, with the inhibitory sequence characterized generally as having an aromatic moiety associated with the penultimate carboxy terminal amino acid, whether it be an aromatic amino acid or another amino acid which has been modified to incorporate an aromatic structure (Goldstein et al . , 1991) . Importantly, the pure inhibitor CVFM (seq id no:34) is the best inhibitor identified to date by the inventors. It should be noted that the related peptide, CFIM (seq id no:33) is not a "pure" inhibitor; its inhibitory activity is due to its action as a substrate for farnesylation. The potency of CVFM peptides as inhibitors of the enzyme may be enhanced by attaching substituents such as fluoro, chloro or nitro derivatives to the phenyl ring. An example is parachlorophenylalanine, which has been tested and found to have "pure" inhibitory activity. It may also be possible to substitute more complex hydrophobic substances for the phenyl group of phenylalanine. These would include naphthyl ring systems. The present inventors propose that additional improvements can be made in pharmaceutical embodiments of the inhibitor by including within their structure moieties which will improve their hydrophobicity, which it is proposed will improve the uptake of peptidyl structures by cells. Thus, in certain embodiments, it is proposed to add fatty acid or polyisoprenoid side chains to the inhibitor which, it is believed, will improve their lipophilic nature and enhance their cellular uptake. Other possible structural modifications include the addition of benzyl, phenyl or acyl groups to the amino acid structures, preferably at a position sufficiently removed from the farnesyl acceptor site, such as at the amino terminus of the peptides. It is proposed that such structures will serve to improve lipophilicity. In this regard, the inventors have found that N-acetylated and N- octylated peptides such as modified CVIM retain much of their inhibitory activity, whereas S-acetoamidated CVIM appears to lose much of its inhibitory activity. Important additional structural characertistics for the preparation of "pure" peptide-based inhibitors have been discovered, which should be taken into account in designing such inhibitors. In particular, it has been found that a positively charged alpha nitrogen at the N- terminus is required for the realization of a "pure" inhibitor. Thus, when such a positive charge is absent, such as through acylation ( e . g. , acetyl or octanoyl group addition) or through N-terminal amino acid addition, and this added structure is not removed mtracellularly or in the test system employed to reveal a positively charged alpha nitrogen on the N-terminal cysteine, the inhibitor is farnesylated and therefore will not serve as a pure inhibitor. Accordingly, where a pure inhibitor is desired, any modification that is made, e . g. , to improve cellular uptake, should take into account the ultimate need for a positively charged alpha nitrogen on the N-terminal cysteine at the site of action. A variety of embodiments are envisioned that will serve to preserve or otherwise reveal a positively charged alpha nitrogen on the N- terminal cysteine upon entry into a cellular target. In general, therefore, it is contemplated that advantages will be realized through the addition of groups to the N- terminal cysteine that will either retain a positive charge on the alpha nitrogen (e.g., alkyl, substituted alkyl, phenyl, benzyl, etc.) or that will reveal such a positively charged nitrogen when removed by normal cellular processes, e.g., cellular enzymes such as oxo- prolinase, esterases, trypsin, chymotrypsin, aminopeptidases, transpeptidases, etc., or by intracellular conditions, such as through hydrolysis or deacylation. Of course, charged species cross cellular membranes only with some difficulty, if at all. Thus, one may desire to employ a carrier composition, such as liposome or carrier molecule, or the addition of a group that will temporarily negate the positive charge. More particularly, in the case of non-removable N- terminal modifications, preferred modifications will be those that retain a positively charged nitrogen, yet which increase hydrophobicity of the peptide. Examples include structures such as R^R^-peptide, wherein R^^ = H, alkyl, phenyl, benzyl, substituted phenyl, substituted benzyl, etc., or even cyclic aza structures such as: N- peptide The generation of removable structures may provide particular advantages. Such structures might advantageously include modifications of the N-terminal cysteine which encompass both the cysteine alpha nitrogen and/or the thiol side chain. Examples would include 2- substituted thiazolidine-4-carboxylic acids, which would undergo intracellular hydrolysis to unmask the cysteine (Nagasawa et al . , 1984) . One such example would be: .A r - * Val-Phe-Meto H H R = H, alkyl, halo-substituted alkyl, phenyl, substituted phenyl, pyridyl or the like. Other structures within this category would include 2- oxo-thiazolidine- -carboxylic acids, that would be cleaved intracellularly at the C-S bond by oxo-prolinases to unmask the cysteine (Hazelton et al . , 1986) . Structures within this category would include: In other embodiments, the invention contemplates removable modifications at the N-terminus based on structures such as : Examples would include acyl modifications of this structure that would be removable by enzymatic cleavage, such as those that include N-acetyl structures wherein R = CH j CO, that are deacylated in vivo or in cell culture (Hazelton et al . , 1986) . Alternatively, phenyl carbamates such as wherein would be cleaved by intracellular esterases, liberating the inhibitor and C0 2 . Other examples would include the inclusion of an N-terminal pyroglutamyl group such as that would be cleaved by pyroglutamyl aminopeptidase to release the inhibitor. Trypsin or chymotrypsin sensitive structures are also contemplated. Trypsin sensitive structures would include the addition of an L-arginine or L-lysine, or even the addition of a protein or peptide that includes an L-lysine or L-arginine carboxy terminus, onto the N- terminus of the tetrapeptide cysteine. An example of such a structure would be as follows, with the site of trypsin sensitivity shown: I©: R f -Arg - Cys-Val-Phe-Met Similar structures are envisioned for conferring chymotrypsin sensitivity, except these would include the amino terminal introduction of L-phenylalanine, L- tyrosine or L-tryptophan moiety, or a peptide or protein including these amino acids. Similarly, gamma-glutamyl derivatives, e . g. , where would be removable by γ-glutamyl transpeptidases. Other modifications that would undergo mtracellular hydrolysis to release a free N-terminus are also contemplated. Examples would include N-mannich base structures such as R'CO-N- "N-peptide * NH 2 — eptide + H H R'CO-NH 2 + CH R = phenyl, sustituted phenyl, alkyl, substituted alkyl, and the like or Schiff bases such as — NH 8 -peptide + R' = p eny! or alkyl V The invention also contemplates that modifications can be made in the structure of inhibitory proteins or peptides to increase their stability within the body, such as modifications that will reduce or eliminate their susceptibility to degradation, e.g., by proteases. For example, the inventors contemplate that useful structural modifications will include the use of amino acids which are less likely to be recognized and cleaved by proteases, such as the incorporation of D-amino acids, or amino acids not normally found in proteins such as ornithine or taurine. Other possible modifications include the cyclization of the peptide, derivatization of the NH groups of the peptide bonds with acyl groups, etc. Assays For Farnesyl:Protein Transferase In still further embodiments, the invention concerns a method for assaying farnesyl transferase (CAAX farnesyltransferase) activity in a composition. This is an important aspect of the invention in that such an assay system provides one with not only the ability to follow the isolation and purification of native or recombinant farnesyl transferase enzymes, but it also forms the basis for developing a screening assay for candidate inhibitors of the enzyme, discussed in more detail below. The assay method generally includes determining the ability of a composition suspected of having farnesyl transferase activity to catalyze the transfer of farnesol to an acceptor protein or peptide. As noted above, a farnesyl acceptor protein or peptide is generally defined as a protein or peptide which will act as a substrate for farnesyl transferase and which includes a recognition site such as -C-A-A-X, as defined above. Typically, the assay protocol is carried out using all-trans farnesyl pyrophosphate as the farnesol donor in the reaction. Thus, one will find particular benefit in constructing an assay wherein a label is present on the farnesyl moiety of all- rans farnesyl pyrophosphate, in that one can measure the appearance of such a label, for example, a radioactive label, in the farnesyl acceptor protein or peptide. As with the characterization of the enzyme discussed above, the farnesyl acceptor sequence which are employed in connection with the assay can be generally defined by -C-A-A-X (seq id no:12) , with preferred embodiments including sequences such as C-V-I-M (seq id no:10) , -C-S- I-M (seq id no:13), -C-A-I-M (seq id no:14) , etc., all of which have been found to serve as useful enzyme substrates. It is believed that most proteins or peptides that include a carboxy terminal sequence of -C- A-A-X (seq id no:12) can be successfully employed in farnesyl protein transferase assays. For use in the assay a preferred farnesyl acceptor protein or peptide will be a p21 ras protein. This is particularly true where one seeks to identify inhibitor substances, as discussed in more detail below, which function either as "false acceptors" in that they divert farnesylation away from natural substrates by acting as substrates in and or themselves, or as "pure" inhibitors which are not in themselves farnesylated. The advantage of employing a natural substrate such as p21 ras is several fold, but includes the ability to separate the natural substrate from the false substrate to analyze the relative degrees of farnesylation. However, for the purposes of simply assaying enzyme specific activity, e.g., assays which do not necessarily involve differential labeling or inhibition studies, one can readily employ short peptides as a farnesyl acceptor in such protocols, such as peptides from about 4 to about 10 amino acids in length which incorporate the recognition signal at their carboxy terminus. Exemplary farnesyl acceptor protein or peptides include but are not limited to CVIM (seq id no:10); KKSKTKCVIM (seq id no:ll); TKCVIM (seq id no:9); RASNRSCAIM (seq id no:15); TQSPQNCSIM (seq id no:16); CUM (seq id no:17); CWM (seq id no:18); and CVLS (seq id no:19). Assays for Candidate Substances In still further embodiments, the present invention concerns a method for identifying new farnesyl transferase inhibitory compounds, which may be termed as "candidate substances." It is contemplated that this screening technique will prove useful in the general identification of any compound that will serve the purpose of inhibiting farnesyl transferase. It is further contemplated that useful compounds in this regard will in no way be limited to proteinaceous or peptidyl compounds. In fact, it may prove to be the case that the most useful pharmacologic compounds for identification through application of the screening assay will be non- peptidyl in nature and, e.g., which will be recognized and bound by the enzyme, and serve to inactivate the enzyme through a tight binding or other chemical interaction. Thus, in these embodiments, the present invention is directed to a method for determining the ability of a candidate substance to inhibit a farnesyl transferase enzyme, the method including generally the steps ' of: (a) obtaining an enzyme composition comprising a farnesyl transferase enzyme that is capable of transferring a farnesyl moiety to a farnesyl acceptor substance; (b) admixing a candidate substance with the enzyme composition; and (c) determining the ability of the farnesyl transferase enzyme to transfer a farnesyl moiety to a farnesyl acceptor substrate in the presence of the candidate substance. An important aspect of the candidate substance screening assay hereof is the ability to prepare a native or recombinant farnesyl transferase enzyme composition in a relative purified form, for example, in a manner as discussed above. This is an important aspect of the candidate substance screening assay in that without at least a relatively purified preparation, one will not be able to assay specifically for enzyme inhibition, as opposed to the effects of the inhibition upon other substances in the extract which then might affect the enzyme. In any event, the successful isolation of the farnesyl transferase enzyme now allows for the first time the ability to identify new compounds which can be used for inhibiting this cancer-related enzyme. The candidate screening assay is quite simple to set up and perform, and is related in many ways to the assay discussed above for determining enzyme activity. Thus, after obtaining a relatively purified preparation of the enzyme, either from native or recombinant sources, one will desire to simply admix a candidate substance with the enzyme preparation, preferably under conditions which would allow the enzyme to perform its farnesyl transferase function but for inclusion of a inhibitory substance. Thus, for example, one will typically desire to include within the admixture an amount of a known farnesyl acceptor substrate such as a p21 ras protein. In this fashion, one can measure the ability of the candidate substance to reduce farnesylation of the farnesyl acceptor substrate relatively in the presence of the candidate substance. Accordingly, one will desire to measure or otherwise determine the activity of the relatively purified enzyme in the absence of the added candidate substance relative to the activity in the presence of the candidate substance in order to assess the relative inhibitory capability of the candidate substance. Methods of Inhibiting Farnesyl:protein Transferase In still further embodiments, the present invention is concerned with a method of inhibiting a farnesyl transferase enzyme which includes subjecting the enzyme to an effective concentration of a farnesyl transferase inhibitor such as one of the family of peptidyl compounds discussed above, or with a candidate substance identified in accordance with the candidate screening assay embodiments. This is, of course, an important aspect of the invention in that it is believed that by inhibiting the farnesyl transferase enzyme, one will be enabled to treat various aspects of cancers, such as ras-related cancers. It is believed that the use of such inhibitors to block the attachment of farnesyl groups to ras proteins in malignant cells of patients suffering with cancer or pre-cancerous states will serve to treat or palliate the cancer, and may be useful by themselves or in conjunction with other cancer therapies, including chemotherapy, resection, radiation therapy, and the like. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1. Transfer of Farnesol from [ 3 H]FPP to p21 H"ras by Partially Purified Rat Brain Farnesyl:Protein Transferase. Each standard assay mixture contained 10 pmoles of [ 3 H] FPP and 3.5 μg of partially purified farnesyl transferase in the absence (A) or presence (•) of 40 μM p21 H"ras . Duplicate samples were incubated for the indicated time at 37°C, and TCA-precipitable radioactivity was measured as described in the Examples. The inset shows the migration on a 12% SDS polyacrylamide gel of an aliquot from a reaction carried out for 1 h in the absence or presence of p21 H"ras . The gel was treated with Entensify solution (DuPont) , dried, and exposed to XAR film for 2 days at -70°C. Figure 2. Substrate Saturation Curves for Farnesyl :Protein Transferase. Panel A: each standard reaction mixture contained 1.8μg of partially purified farnesyl transferase, 40μg p21 H"ras , [ 3 H] FPP (250,000 dpm) ; and varying amounts of unlabeled FPP to give the indicated final concentration of [ 3 H]FPP. Panel B: each standard reaction mixture contained 3.2μg partially purified farnesyl transferase, lOpmol [ 3 H]FPP, and the indicated concentration of p21 H"ras that had been incubated with 50μM of the indicated nucleotide for 45 min at 30°C and then passed through a G-50 Sephadex gel filtration column at room temperature in buffer containing 10 mM Tris-chloride (pH 7.7), ImM EDTA, ImM DTT, and 3mM MgCl 2 . For both panels, assays were carried out in duplicate for 1 hour at 37°C, and TCA-precipitable radioactivity was measured as described in the Example. Figure 3. Divalent Cation Requirement for Farnesyl :Protein Transferase. Each standard reaction mixture contained lOpmol [ 3 H]FPP, 2.5μg of partially purified farnesyl transferase, 40μM p21 H"ras , 0.15mM EDTA, and the indicated concentrations of either ZnCl 2 (•) or MgCl 2 (A) . Incubations were carried out in duplicate for 1 hour at 37°C, and TCA-precipitable radioactivity was measured as described in the Examples. Figure 4. Identification of [ 3 H] FPP-derived Radioactive Material Transferred to p21 H"ras . Panel A: an aliquot from a standard reaction mixture was subjected to cleavage with methyl iodide as described in the Examples. Panel B: another aliquot was treated identically except methyl iodide was omitted. After cleavage, the extracted material was dried under nitrogen, resuspended in 0.4ml of 50% (v/v) acetonitrile containing 25mM phosphoric acid and 6 nmoles of each isoprenoid standard as indicated. The mixture was subjected to reverse phase HPLC (C18, Phenomex) as described by Casey, et al . (1989) except that an additional 10-min wash with 100% acetonitrile/phosphoric acid was used. The isoprenoid standards were identified by absorbance at 205 nm: C 10 , all-trans geraniol; C 15 , all-trans farnesol; C 20 , all-trans geranylgeraniol. Figure 5. Chromatography of Farnesyl:Protein Transferase on a Mono Q Column. The 30-50% ammonium sulfate fraction from rat brain (200mg) was applied to a Mono Q column (10 x 1-cm) equilibrated in 50mM Tris- chloride (pH 7.5) containing ImM DTT, 20μM ZnCl 2 , and 0.05M NaCl . The column was washed with 24ml of the same buffer containing 0.05M NaCl, followed by a 24-ml linear gradient from 0.05 to 0.25M NaCl, followed by a second wash with 24 ml of the same buffer containing 0.25M NaCl. The enzyme was then eluted with a 112-ml linear gradient of the same buffer containing 0.25-1.0M NaCl at a flow rate of 1 ml/min. Fractions of 4ml were collected. An aliquot of each fraction (2μl) was assayed for farnesyl :protein transferase activity by the standard method (o) . The protein content of each fraction (•) was estimated from the absorbance at 280 mM. Figure 6A. SDS Polyacrylamide Gel Electrophoresis of Farnesyl:Protein Transferase at Various Stages of Purification. 10μg of the 30-50% ammonium sulfate fraction (lane 1) , 3μg of the Mono Q fraction (lane 2) , and approximately 90ng of the peptide affinity-column fraction (lane 3) were subjected to SDS-10% polyacrylamide gel electrophoresis, and the protein bands were detected with a silver stain. The farnesyl:protein transferase activity in each sample loaded onto the gel was approximately 0.1, 0.8, and 54 units/lane for lanes 1, 2, and 3, respectively. The molecular weights for marker protein standards are indicated. Conditions of electrophoresis: 10% mini gel run at 30 mA for 1 hour. Figure 6B. SDS Polyacrylamide Gel Electrophoresis of Purified Farnesyl :Protein Transferase. 0.7μg of the peptide affinity-purified-column fraction (right lane) was subjected to SDS-10% polyacrylamide gel electrophoresis, and the protein bands were detected with a Coomassie Blue Stain. The molecular weights for marker protein standards (left lane) are indicated. Conditilank was 3.78pmol of t 3 H] FPP p21 H'ras formed per hour. Peptides A, o and o correspond to the COOH-terminal 10, 6, and 4 amino acids of wild-type human p21 H"ras protein, respectively. Peptides D and A are control peptides. Figure 7. Gel Filtration of Farnesyl :Protein Transferase. Affinity-purified farnesyl transferase (- 1 μg protein) was subjected to gel filtration on a Superose-12 column (25 x 0.5-cm) in 50 mM Tris-chloride (pH 7.5) containing 0.2 M NaCl, 1 mM DTT, and 0.2% octyl- 3-D-glucopyranoside at a flow rate of 0.2 ml/min. Fractions of 0.5 ml were collected. Panel A. a 6-μl aliquot of each fraction was assayed for farnesyl :protein transferase activity by the standard method except that each reaction mixture contained 0.2% octyl-0-D- glucopyranoside. The column was calibrated with thyroglobulin (670 kDa) , γ-globulin (158 kDa) , ovalbumin (44 kDa) , myoglobin (17 kDa) , and vitamin B12 (1.35 kDa) . Arrows indicate the elution position of the 158-kDa and 44-kDa markers. Panel B. a 0.42-ml aliquot of each fraction was concentrated to 40 μl with a Centricon 30 Concentrator (Amicon) , and 25 μl of this material was then subjected to electrophoresis on an 10% SDS polyacrylamide gel . The gel was stained with silver nitrate and calibrated with marker proteins (far-right lane) . Figure 8. Inhibition of Farnesyl :Protein Transferase Activity by Peptides. Each standard reaction mixture contained 10 pmol [ 3 H]FPP, 1.8 μg of partially purified farnesyl :protein transferase, 40 μM p21 H'mr , and the indicated concentration of competitor peptide added in 3 μl of 10 mM DTT. After incubation for 1 h at 37°C, TCA-precipitable radioactivity was measured as described in Experimental Procedures. Each value is the mean of triplicate incubations (no peptide) or a single incubation (+ peptide) . A blank value of 0.11 pmol/h was determined in a parallel incubation containing 20 mM EDTA. This blank was subtracted from each value before calculating "% of control" values. The "100% of control" value after subtraction of the blank was 3.78 pmol of [ 3 H] FPP p21 H"rαr formed per h. Peptides Δ, O and • correspond to the COOH-terminal 10, 6, and 4 amino acids of wild-type human p21 H" "* protein (seq id nos:10, 9 and 11) , respectively. Peptides D (CNFDNPVSQKTT; seq id no:48) and A (TKVCIM; seq id no:49) are control peptides. Figure 9. Inhibition of Farnesyl :Protein Transferase Activity by Peptides. Incubations were carried out exactly as described in the legend to Figure 8. The "100% of control value" was 2.92pmol of [ 3 H] farnesyl p21 H"fas formed per hour. The blank value was 0.20pmol/h. Each peptide consisted of the COOH-terminal 10 residues of the indicated protein. Peptide KNNLKDCGLF is seq id no:50; KKSKTKCVIM is seq id no:11; TQSPQNCSIM is seq id no:16; and RASNRSCAIM is seq id no:15. Figure 10. Inhibition of Farnesyl :Protein Transferase By Tetrapeptide Analogues of CVIM (seq id no:10) . The standard assay mixture contained 15pmol [ 3 H]FPP, 4 to 7.5μg partially purified farnesyl transferase, 30 or 40μM p21 H"ras , and the indicated concentration of competitor tetrapeptide. After 30 or 60 min, the amount of [ 3 H] farnesyl attached to p21 H"ras was measured by trichloracetic acid precipitation as described in the methods section of Example II. Each value is the average of duplicate or triplicate incubations (no peptide) or a single incubation (+peptide) . Each tetrapeptide was tested in a separate experiment together with equivalent concentrations of CVIM (seq id no:10) . The values for inhibition by CVIM ( ) represent mean values from 21 experiments in which the mean "100% of control" value was 13 pmol min "x mg protein "1 . K concentration of tetrapeptide giving 50% inhibition. Represented are CAIM (seq id no:14) ; CVIA (seq id no:25) ; CVAM (seq id no:30) ; CKIM (seq id no:31) ; CLIM (seq id no:32); CVLM (seq id no:20); CVIL (seq id no:26) ; CVKM (seq id no:51) ; and CVIK (seq id no:52) . Figure 11. Inhibition of Farnesyl:Protein Transferase Activity By Phenylalanine-Containing Analogues of CVIM (seq id no:10) . Enzyme activity was measured in the presence of the indicated concentration of competitor tetrapeptide as described in the legend to Figure 10. Represented are CFIM (seq id no:33) ; CVFM (seq id no:34) ; and CVIF (seq id no:35) . Figure 12. Inhibition of Farnesylation of p21 H " ras (A) and Biotinylated KTSCVIM (seq id no:53) (B) By CVFM (seq id no:34) . Panel A: Each reaction mixture contained 15pmol [ 3 H]FPP, 4.5 or 6ng of purified farnesyl :protein transferase, 40μM p21 H"ras , and the indicated concentration of competitor tetrapeptide. After incubation for 30 min at 37°C, the amount of t 3 H] farnesyl transferred to p21 H"ras was measured by the standard filter assay. Values shown are the average of two experiments. The "100% of control" values were 16 and 19 nmol min "1 mg protein "1 , Panel B: Each reaction contained 15 pmol [ 3 H]FPP, 4.5 or 6ng of purified farnesyl :protein transferase, 3.4μM biotinylated KTSCVIM (seq id no:53) , and the indicated concentration of competitor tetrapeptide. After incubation for 30 min at 37°C, the [ 3 H] farnesyl-labeled peptide was trapped on streptavidin-agarose, washed, separated from the unincorporated [ 3 H]FPP, and subjected to scintillation counting. Values shown are the mean of 3 experiments. The "100% of control" values were 10, 17, and 21 nmol min^mg protein "1 . Represented are CVFM (seq id no:34) and CVIM (seq id no:10) . Figure 13. Inhibition of Farnesyl :Protein Transferase By Modified Tetrapeptides. Enzyme activity was measured in the presence of varying concentrations of the indicated tetrapeptide as described in the legend to Figure 10. The "100% of control" values were 9.3 and 9.2 pmol min "1 mg protein "1 in Panels A and B, respectively. Figure 14. Inhibition of Farnesyl :Protein Transferase By Tetrapeptides With Single Amino Acid Substitutions in CVIM (seq id no:10) . Enzyme activity was measured in the presence of the indicated competitor tetrapeptide as described in the legend to Figures 10 and 11. Each tetrapeptide was tested at seven different concentrations ranging from 0.01 to lOOμM. The concentration of tetrapeptide giving 50% inhibition was calculated from the inhibition curve. The single and double underlines denote tetrapeptides corresponding to the COOH-terminal sequence of mammalian and fungal proteins, respectively, that are candidates for farnesylation (see Table III) . CXIM is seq id no:54; CVXM is seq id no:55 and CVIX is seq id no:56. Figure 15. Farnesylation of CVIM (seq id no:10) but not CVFM (seq id no:34) by Purified Farnesyl :protein Transferase. The standard assay mixture (25μl) contained 17pmol t 3 H] FPP (44,000 dpm/pmol) , 5 ng of purified farnesyl:protein transferase, 0.2% (w/v) octyl-β-D- glucoside, and 3.6μM of the indicated tetrapeptide. After incubation for 15 min at 37°C, the entire reaction mixture was subjected to thin layer chromatography for 4 hours on Polygram SIL G sheet (Brinkmann Instruments) in a solvent system containing N-propanol/concentrated NH 4 OH/water (6:3:1) . The TLC sheet was then dried, sprayed with ENHANCE Spray (Dupont-New England Nuclear) and exposed to Kodak X-OMAT AR Film XAR-5 for 25 hours at -70°C. Figure 16. cDNA Probes Generated from a Knowledge of the Amino Acid Sequences of Peptides Derived from Rat Farnesyl Transferase α. and β Subunits. Panel A: Primer cϋl (seq id no:57) and Primer α.2 (seq id no:58) were used in PCR with rat genomic DNA to obtain the nucleotide sequence encoding the amino acid sequence of the peptide shown (seq id no:59) , as described in Example III. The nucleotide sequence 5'-ATIGAGTTAAACGCAGCCAACTATACGGTCTGGCACTT-3' (a specific example in accordance with residues 6-54 of seq id no:64), was used as a probe to screen a rat brain cDNA library. Panel B (upper) : Primer βl (seq id no:60) and primer β2 (seq id no:61) were used in PCR with rat genomic DNA to generate the nucleotide sequence encoding the amino acid sequence of the peptide shown (seq id no:63) , as described in Example III. Panel B (lower) : Nucleotide sequence encoding the peptide as derived from the above PCR (seq id no:62) . Primer β3 and primer β4, the sequences of which are contained entirely within seq id no:62, were synthesized and used as the primers for 3'-end amplification of the cDNA, as described in Example III. Figure 17. Identification of the Amino Acids Within the Sequence of Rat Farnesyl Transferase o; Subunit (FT-α.) (seq id no:l) which are Identical with those within the Sequence of Yeast RAM2. Amino acid residues are numbered on the left. Identical amino acids are boxed. The sequence of yeast RAM2 has been reported by He et al . (1991) , and the non-identical residues are not shown. Figure 18. Identification of the Amino Acid Within the Sequence of Rat Farnesyl Transferase β-Subunit (FT-β) (seq id no:3) which are Identical with those within the Sequence of Yeast DPR1/RAM1. Amino acid residues are numbered on the left. Identical amino acids are boxed. The sequence of yeast DPR1/RAM1 has been reported by Goodman et al . (1988), and the non-identical residues are not shown. Figure 19. Distribution of Rat Farnesyl Transferase 0! and β subunit mRNA in Tissues (A &_ C) and Cultured Cells (B & D) . Panels A & C: Total RNA was isolated from the indicated rat tissues, and an aliquot (30μg) was subjected to electrophoresis on a 1.5% agarose gel and blotted onto a nylon membrane for blot analysis. Hybridization was carried out at 42°C for 20 hours with a mixture of two single-stranded uniformly 32 P-labeled cDNA probes, specific for either the α subunit (A) or β subunit (B) of rat farnesyl transferase. Each probe was ~500 nucleotides in length and was used at 2 x IO 6 cpm/ml. The filters were washed in 0.2x SSC containing 0.2% (w/v) SDS at 68°C for 1 hour, then exposed to Kodak XAR-5 film for 2-4 days at -70°C. The positions of RNA standards run in adjacent lanes are indicated on the left. As a loading control, the same filter was reprobed initially with a 32 P-labeled 49-mer oligonucleotide corresponding to rat cyclophilin cDNA (2 x IO 6 cpm/ml) and subsequently with a uniformly 32 P-labeled cDNA (-1.2 kb) for rat glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (4 x 10 6 cpm/ml) . After each washing, the reprobed filter was exposed for 12 hours at -70°C. Panels B & D: Expression of the (C) and β (D) farnesyl transferase subunit mRNA in rat brain, KNRK cells, and PC12 pheochromocytoma cells. An aliquot of poly(A) + RNA from each sample (lOμg) was subjected to blot analysis as described in A & B, and exposed for 12 h at -70°C. The same filter was subsequently reprobed with a 32 P- oligonucleotide derived from the rat cyclophilin cDNA sequence as described in A & B, and the filter was exposed to XAR-5 film for 12 h at -70°C. Figure 20. Immunoblot Analyses of a and /3-subunits of Rat Protein Farnesyl Transferase Expressed in Transfected 293 cells. Samples were subjected to SDS/PAGE on 10% gels and transferred to nitrocellulose. The filters were incubated with either lμg/ml of rabbit anti a subunit IgG-Y533 (A) or 5μg/ml of rabbit anti β subunit IgG-X287 (B) followed by incubation with 125 I- labeled goat anti-rabbit IgG (lxlO 6 cpm/ml) . Lanes 1 and 3_, 20μg of partially purified Mono Q fraction of rat brain farnesyl transferase. Lanes 2,4,5,6,7. 20μg of cytosol from 293 cells transfected with the following piasmids: pFT-o. plus pFT-3l (lanes 2 and 7) ; pFT-α. plus pFT-/3lrev (lane 4) ; pFT-cϋrev plus pFT-βl (lane 5) ; pFT-cϋrev plus pFT-βlrev (lane 6) . The filters were exposed to Kodak XAR-5 film for 48 h (A) or 16 h (B) at - 70°C. Molecular weight markers are indicated. Piasmids pFT-αrev and pFT-βlrev contain cDNAs inserted in the reverse (noncoding) orientation. Figure 21. Farnesyl Transferase Activity of Cytosolic Extracts from 293 cells Transfected with cDNAs Encoding the cn and β Subunits of Rat Protein Farnesyl Transferase in the Correct or Reverse (rev) Orientations. Cells were transfected with 3μg of the indicated plasmid plus lμg pVA. Each assay contained in a final volume of 25μl the indicated amount of cytosolic extract, 50mM Tris-chloride (pH 7.5) , 50μM ZnCl 2 , 20mM KCl, 3mM MgCl 2 , ImM dithiothreitol, 0.4% (v/v) octyl-jS-glucopyranoside, 40μM p21 H " ras , and 15pmol of all- rans [ 3 H] farnesyl pyrophosphate (15,335 dpm/pmol) . Assay tubes were incubated at 37°C for 10 min, after which the amount of [ 3 H] farnesyl attached to p21 H"ras was measured. Each value is the average of duplicate incubations. Figure 22. Schematic Diagram of the Reaction Sequence for EDTA-treated Protein Farnesyltransferase. Figure 23. Differential interaction of protein farnesyltransferase with CVFM (•) and N-AcCVFM (A) with protein farnesyltransferase. Panel A, Inhibition of farnesylation of p21 H"ras . The standard assay mixture contained 40 μM p21 H"ras , 5 μg farnesyltransferase (Mono Q fraction), 0.5 μM [ 3 H] farnesyl pyrophosphate (15,178 dpm/pmol) , and the indicated concentration of CVFM (•) or N-AcCVFM (A) . After incubation for 30 min at 37°C, the amount of [ 3 H] farnesyl attached to p21 H"ras was measured following trichloracetic acid precipitation. Each value is the average of duplicate incubations. The "100% of control" value was 24.3 pmol/min per mg protein. Panel B, Farnesylation of tetrapeptides. Each reaction mixture contained 2.4 μM [ 3 H] farnesyl pyrophosphate (9515 dpm/pmol) , ~ 5 ng of affinity-purified protein farnesyltransferase, and the indicated concentration of CVFM (•) or N-AcCVFM (A) . After incubation for 15 min at 37°C, the reaction mixture was subjected to thin layer chromatography and quantified as described in the examples. Blank values in parallel reactions containing SVIM (a nonfarnesylated peptide) at concentrations of 0.4, 1.2, and 3.6 μM (0.65, 0.76, and 0.57 pmol/tube, respectively) were subtracted from the corresponding experimental values to give the values shown for CVIM and N-AcCVIM . Figure 24. Differential interactions of CIFM (•) and N-AcCIFM (A) with protein farnesyltransferase. Panel A, Inhibition of farnesylation of p21 H"ras . Panel B, Farnesylation of tetrapeptides. These experiments were carried out under identical concentrations to those described in the legend to Fig. 23. Figure 25. Reduced farnesylation of N-OctanoylCVFM in presence of CVFM. Each reaction mixture contained 2.4 μM [ 3 H] farnesyl pyrophosphate (11,574 dpm/pmol) , ~ 5 ng of affinity-purified protein farnesyltransferase, and the indicated concentration of N-OctanoylCVFM in the absence (•) or presence (A) of 3.6 μM CVFM. After incubation for 15 min at 37°C, the reaction mixture was subjected to thin layer chromatography and quantified as described in Example V. A blank value of 0.58 pmol/tube was determined in parallel reactions containing no peptide, and this blank was subtracted from each experimental value. Figure 26. Nucleotide Sequence (seq id no:6) and Deduced Amino Acid Sequence (seq id no:5) of a Full Length cDNA Encoding the Human Farnesyl Transferase o; Subunit, and Comparison with the Amino Acid Sequence of the Rat α Subunit. Amino acids are numbered on the left. Amino acid residue 1 is the putative initiator methionine. The translated 379 amino acid sequence of the human farnesyl transferase cn subunit protein (seq id no:5) is shown beneath the nucleotide sequence (seq id no:6) . Amino acid residues that differ from the rat protein are boxed and the corresponding amino acids in the rat sequence are shown below the human sequence. Figure 27. Nucleotide (seq id no: 8) and Deduced Amino Acid Sequence (seq id no:7) of a Partial cDNA Encoding the Human Farnesyl Transferase β Subunit, and Comparison with the Amino Acid Sequence of the Rat β Subunit. Nucleotides are numbered on the right. Amino acids are numbered on the left with the number in parentheses indicating the corresponding residue in the rat protein. The translated 387 amino acid sequence (seq id no:7) of the partial human farnesyltransferase β subunit cDNA is shown beneath the nucleotide sequence. Amino acids that differ from the rat protein are boxed and the differences are shown below the human sequence. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following examples illustrate techniques discovered by the inventors for the identification and purification of mammalian farnesyl protein transferase enzymes, as well as techniques for their assay and for the screening of new compounds which may be employed to inhibit such enzymes. These studies also demonstrate a variety of peptidyl compounds which themselves can be employed to inhibit these enzymes. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent laboratory techniques discovered by the inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention. EXAMPLE I PREPARATION AND CHARACTERIZATION OF FARNESYL:PROTEIN TRANSFERASE 1. Materials Peptides were obtained from Peninsula Laboratories or otherwise synthesized by standard techniques. All peptides were purified on HPLC, and their identity was confirmed by amino acid analysis. Just prior to use, each peptide was dissolved at a concentration of 0.8mM in • lOmM dithiothreitol (DTT) , and all dilutions were made in lOmM DTT. Unlabeled all- rans farnesyl pyrophosphate (FPP) was synthesized by the method of Davisson, et al . (1986) . [1- 3 H] Farnesyl pyrophosphate (20 Ci/mmol) was custom synthesized by New England Nuclear. Geraniol and farnesol (both all-trans) were obtained from Aldrich Chemical. All-trans geranylgeraniol was obtained from R. Coates (University of Illinois) . Recombinant wild type human p21 H " ras protein was produced in a bacterial expression system with pAT-rasH (provided by Channing J. Der, La Jolla Cancer Research Foundation, La Jolla, CA) , an expression vector based on pXVR (Feig et al. , 1986) . The plasmid was transformed into E. coli JM105, and the recombinant p21 H " ras protein was purified at 4°C from a high speed supernatant of the bacterial extracts by sequential chromatography on DEAE- Sephacel and Sephadex G-75. Purity was -90% as judged by Coomassie blue staining of SDS gels. Purified p21 H " ras was concentrated to 15mg/ml in lOmM Tris-chloride (pH 7.5) containing ImM DTT, ImM EDTA, 3mM MgCl 2 , and 30μM GDP and stored in multiple aliquots at -70°C. 2. Assay for Farnesyl: rotein Transferase Activity Farnesyl :protein transferase activity was determined by measuring the amount of 3 H-farnesol transferred from all- trans 3 H] farnesyl pyrophosphate ([ 3 H]FPP) to p21 H"ras protein. The standard reaction mixture contained the following concentrations of components in a final volume of 25μl: 50mM Tris-chloride (pH 7.5) , 50μM ZnCl 2 , 20mM KC1, ImM DTT, and 40μM p21 H'ras . The mixture also contained lOpmoles of [ 3 H] FPP (-30,000 dpm/pmol) and 1.8-3.5μg of partially purified farnesyl :protein transferase (see below) . After incubation for 1 hour at 37°C in 12 x 75- mm borosilicate tubes, the reaction was stopped by addition of 0.5ml of 4% SDS and then 0.5ml of 30% trichloroacetic acid (TCA) . The tubes were vortexed and left on ice for 45-60 min, after which 2ml of a 6% TCA/2% SDS solution were added. The mixture was filtered on a 2.5-cm glass fiber filter with a Hoefer filtration unit (FH 225) . The tubes were rinsed twice with 2ml of the same solution, and each filter was washed five times with 2ml of 6% TCA, dried, and counted in a scintillation counter. One unit of activity is defined as the amount of enzyme that transfers lpmol of [ 3 H] farnesol from [ 3 H] FPP into acid- precipitable p21 H"ras per hour under the standard conditions. 3. Purification of Farnesyl:Protein Transferase All steps were carried out at 4°C except where indicated: Step 1 - Ammonium Sulfate Fractionation: Brains from 50 male Sprague-Dawley rats (100-150 g) were homogenized in 100ml of ice-cold buffer containing 50mM Tris-chloride (pH 7.5) , ImM EDTA, ImM EGTA, 0.2mM phenylmethylsulfonyl fluoride (PMSF) , and 0.ImM leupeptin, and the extract was spun at 60,000 x g for 70 min. The supernatant was brought to 30% saturation with solid ammonium sulfate, stirred for 30 min on ice, and centrifuged at 12,000 x g for 10 min to remove precipitated proteins. The resulting supernatant was adjusted to 50% saturation with ammonium sulfate, and the resulting pellet was dissolved in ~20ml of 20mM Tris-chloride (pH 7.5) containing ImM DTT and 20μM ZnCl 2 and dialyzed for 4 hours against 4 liters of the same buffer and then 4 liters of fresh buffer of the same composition for 12 hours. The dialyzed material was divided into multiple aliquots and stored at -70°C. Step 2 - Ion-exchange Chromatography: A portion of the 30-50% ammonium sulfate fraction (200mg protein) was chromatographed on a Mono Q 10/10 column using an FPLC system (Pharmacia LKB Biotechnology) . The column was run as described in the legend to Figure 5. Fractions eluting between 0.3 and 0.4M NaCl contained the majority of the transferase activity. These fractions were pooled, divided into multiple aliquots, and stored at - 70°C. Step 3 - Affinity Chromatography: An affinity column containing a peptide corresponding to the COOH- terminal six amino acids of p21 κ"ras"B protein was prepared as follows. Fifteen mg of the peptide TKCVIM (seq id no:9) were coupled to 1 g of activated CH-Sepharose 4B (Pharmacia LKB Biotechnology) according to the manufacturer's instructions. The resulting 2.5-ml slurry was poured into a column, and excess uncoupled peptide was removed by 10 cycles of alternating washes, each consisting of 40 column volumes of 0.1M sodium acetate (pH 4.0) and then 0.1M Tris-chloride (pH 8.0) . Both buffers contained 1M NaCl and lOmM DTT. The column was stored at 4°C in 20mM Tris-chloride (pH 7.2) and 0.02% sodium azide. Fifteen mg of Mono Q-purified material in 10ml were applied to a 1-ml peptide column equilibrated in 50mM Tris-chloride (pH 7.5) containing 0.IM NaCl and ImM DTT (Buffer A) . The enzyme-containing solution was cycled through the column three times at room temperature. The column was washed with 20ml of Buffer A containing 0.2% (w/v) octyl-β-D-glucopyranoside (Buffer B) . The enzyme was eluted with 20ml of 50mM Tris- succinate (pH 5.0) containing ImM DTT, 0. IM NaCl, and 0.2% octyl-β-D-glucopyranoside. The pH 5 eluate was concentrated and washed twice with a 10-fold excess of Buffer B in a CF25 Centriflo ultrafiltration cone (Amicon) and brought to 1ml (10-fold concentration relative to the starting material) . Step 4 - Gel Filtration: Affinity-purified farnesyl transferase (~lμg) was chromatographed on a Superose 12 column as described in the legend to Figure 7. In the enzyme characterization experiments of Figs. 1-4, 8, and 9, a partially purified fraction of farnesyl :protein transferase was used. This enzyme was prepared by Steps 1 and 2 as described above, after which 6 mg of the Mono Q-purified material was concentrated to 2ml and then loaded onto a 1.6 x 50-cm Sephacryl S-200 high resolution gel filtration column (Pharmacia LKB Biotechnology) . The column was equilibrated with 50mM Tris-chloride (pH 7.5) containing ImM DTT, 0.2M NaCl, 20μM ZnCl 2 , and 0.2% octyl-/3-glucopyranoside and eluted with the same buffer at a flow rate of 15 ml/hour. Only the peak fraction, containing lmg protein and 40% of initial activity, was used for studies. 4. Identification of 3 H-Isoprenoid Transferred from r 3 HlFPP A modification of the procedure described by Casey et al . (Casey et al . , 1989) was employed as follows: Briefly, two standard transferase reactions of 25-μl each were conducted for 1 hour at 37°C. The mixtures were then pooled, and a 25-μl aliquot from the 50-μl pooled sample was diluted to 250μl with 2% (w/v) SDS. This mixture was precipitated with an equal volume of 30% TCA, filtered through nitrocellulose, (7mm disc) , washed twice with 250μl 6% TCA/2% SDS followed by five washes with 5% TCA, digested with 8μg trypsin, and subjected to cleavage with methyl iodide. The released 3 H-isoprenoids were extracted into chloroform/methanol and chromatographed on a reverse-phase HPLC system as described in the legend to Figure . 5. Other Methods SDS polyacrylamide gel electrophoresis was carried out as described by Laemmli (Laemmli, 1970) . Gels were calibrated with high range SDS-PAGE standards (Bio-Rad) . Protein content of extracts was measured by the method of Lowry, et al . (Lowry et al. , 1951) except for that of the affinity-purified material, which was estimated by comparison to the bovine serum albumin marker (M r 66,000) following SDS gel electrophoresis and Coomassie staining. 6. Results and Discussion As an initial attempt to identify a farnesyl protein transferase enzyme, rat brain cytosol was fractionated with ammonium sulfate and the active fraction subjected to ion exchange chromatography on a Mono Q column followed by gel filtration on Sephacryl S-200. Figure 1 shows that the active fraction from this column incorporated radioactivity from [ 3 H] farnesol into trichloroacetic acid precipitable p21 H"ras in a time- dependent fashion at 37°C. The incorporated radioactivity could be visualized as a band of the expected molecular weight of -21 kDa on SDS polyacrylamide gels (inset) . The concentration of [ 3 H] farnesyl pyrophosphate that gave half-maximal reaction velocity was approximately 0.5μM (Figure 2A) . The half- maximal concentration for p21 Hras was approximately 5μM, and there was no difference when the p21 H"ras was equilibrated with a nonhydrolyzable GTP or ATP analogue or with GDP (Figure 2B) . With p21 H"ras as a substrate, the transferase reaction was inhibited by 0.15mM EDTA, and this inhibition was reversed by 0.1 to 1. OmM concentrations of zinc or magnesium chloride (Figure 3) . At higher concentrations of zinc chloride, inhibition was observed. To confirm that the transferred material was [ 3 H] farnesol, the washed trichloracetic acid-precipitated material was digested with trypsin, the radioactivity released with methyl iodide, and the products subjected to reverse-phase HPLC. The methyl iodide-released material co-migrated with an authentic standard of all- trans farnesol (C !5 ) (Figure 4A) . Some radioactivity emerged from the column prior to the geraniol standard (C 10 ) , but this was the same in the presence and absence of methyl iodide treatment. This early-eluting material was believed to represent some tryptic peptides whose radioactivity was not released by methyl iodide. Figure 5 shows the elution profile of farnesyl transferase activity from a Mono Q column. The activity appeared as a single sharp peak that eluted at approximately 0.35M sodium chloride. The peak fractions from the Mono Q column were pooled and subjected to affinity chromatography on a column that contained a covalently-bound peptide corresponding to the carboxyl-terminal 6-amino acids of p21 κ"rasB . All of the farnesyl transferase activity was adsorbed to the column, and about 50% of the applied activity was recovered when the column was eluted with a Tris-succinate buffer at pH 5.0. Table II summarizes the results of a typical purification procedure that started with 50 rat brains. After ammonium sulfate precipitation, mono Q chromatography, and affinity chromatography, the farnesyl transferase was purified approximately 61, 000-fold with a yield of 52%. The final specific activity was about 600,000 units/mg. Figure 6A shows the SDS gel electrophoretic profile of the proteins at each stage of this purification as visualized by silver staining. The peptide affinity column yielded a single protein band with an apparent subunit molecular weight of 50,000. When the purified enzyme was subjected to SDS gel electrophoresis under more sensitive conditions, the 50-kDa protein could be resolved into two closely spaced bands that were visualized in approximately equimolar amounts (Figure 6B) . To confirm that the 50-kDa band was the farnesyl transferase enzyme, the affinity column purified material was subjected to gel filtration. Figure 7 shows that the farnesyl transferase activity and the 50-kDa band co- eluted from this column at a position corresponding to an apparent molecular weight of 70-100 kDa as determined from the behavior of markers of known molecular weight . TABLE II PURIFICATION OF FARNESY :PROTEIN TRANSFERASE FROM RAT BRAIN I 10 The purification procedure was started with 50 rat brains. a One unit of enzyme activity is the amount of enzyme that transfers 1 pmol of [ 3 H] farnesol from [ 3 H] FPP into acid-precipitable p21 H" ™ per h under the standard conditions. 15 Protein concentration was estimated by coomassie blue staining of a SDS polyacrylamide gel using various amounts (0.5 to 2 μg) of bovine serum albumin as a reference standard. The adherence of the farnesyl transferase to the peptide affinity column suggested that the enzyme was capable of recognizing short peptide sequences. To test for the specificity of this peptide recognition, the ability of various peptides to compete with p21 H"ras for the farnesyl transferase activity was measured. The peptide that was used for affinity chromatography corresponded to the carboxyl terminal six amino acids of P2l κ " rasB (TKCVIM; seq id no:9) . As expected, this peptide competitively inhibited farnesylation of P21 H"ras (open circles in Figure 8) . The terminal 4-amino acids in this sequence (CVIM; seq id no:10) (closed circles) were sufficient for competition. These two short peptides were no less effective than a peptide that contained the final 10- amino acids of the sequence (KKSKTKCVIM; seq id no:11) (open triangles) . The simple transposition of the cysteine from the fourth to the third position from the COOH-terminus of the hexapeptide (TKVCIM; seq id no:9) (closed triangles) severely reduced inhibitory activity. An irrelevant peptide (closed squares) also did not inhibit. Figure 9 compares the inhibitory activities of four peptides of 10-amino acids each, all of which contain a cysteine at the fourth position from the COOH-terminus. The peptides corresponding to the COOH-terminus of human p21 κ"rasB and human lamin A and lamin B all inhibited farnesylation. All of these peptides are known to be prenylated in vivo (Casey et al . , 1989; Farnsworth et al . 1989) . On the other hand, the peptide corresponding to the sequence of rat Giαl, a 40kDa G protein that does not appear to be farnesylated in vivo, did not compete for the farnesyl transferase reaction. In data not shown it was found that the 10-amino acid peptide corresponding to the COOH-terminus of p21 H " ras (CVLS seq id no:19) , p21 N"ras (CWM; seq id no:18) , and p21 H " rasA (CUM; seq id no:17) all competed for the farnesylation reaction. EXAMPLE II FURTHER CHARACTERIZATION OF FARNESYL:PROTEIN TRANSFERASE In the present Example, a series of tetrapeptides were tested for their ability to bind to the rat brain p21 H"ras farnesyl:protein transferase as estimated by their ability to compete with p21 H"ras in a farnesyl transfer assay. Peptides with the highest affinity had the structure Cys-Al-A2-X, where Al and A2 are aliphatic amino acids and X is a C-terminal methionine, serine, or phenylalanine. Charged residues reduced affinity slightly at the Al position and much more drastically at the A2 and X positions. Effective inhibitors included tetrapeptides corresponding to the COOH-termini of all animal cell proteins known to be farnesylated. In contrast, the tetrapeptide CAIL (seq id no:65) , which corresponds to the COOH-terminus of the only known examples of geranylgeranylated proteins (neural G protein subunits) did not compete in the farnesyl transfer assay, suggesting that the two isoprenes are transferred by different enzymes. A biotinylated hexapeptide corresponding to the COOH-terminus of p21 κ " rasB was farnesylated, suggesting that at least some of the peptides serve as substrates for the transferase. The data are consistent with a model in which a hydrophobic pocket in the farnesyl :protein transferase recognizes tetrapeptides through interactions with the cysteine and the last two amino acids. 1. Materials and Methods a. Peptides Peptides were prepared by established procedures of solid-phase synthesis (Stewart et al . , 1984) Tetrapeptides were synthesized on the Milligen 9050 Synthesizer using Fmoc chemistry. After deprotection of the last residue, a portion of the resin was used to make the N-acetyl-modified version of CVIM. This was done off-line in a solution of acetic anhydride and dimethylformamide at pH 8 (adjusted with diisopropylethylamine) . The acetylated and unacetylated peptides were cleaved with 50ml of trifluoroacetic acid:phenol (95:5) plus approximately 1ml of ethanedithiol added as a scavenger. The N-octyl-modified version of CVIM was synthesized on an Applied Biosystems Model 430 Synthesizer using tBoc chemistry. The octyl group was added in an amino acid cycle using octanoic acid. The peptide was cleaved from the resin at 0°C with a 10:1:1 ratio of HF (mls) :resin (g) :anisole (ml) . The peptides were purified by high pressure liquid chromatography (HPLC) on a Beckman C18 reverse phase column (21.1 cm x 15 cm) , eluted with a water- acetonitrile gradient containing 0.1% (v/v) trifluouroacetic acid. Identity was confirmed for all peptides by fast atom bombardment (FAB) mass spectrometry. Just prior to use, each peptide was dissolved at a concentration of 0.8mM in lOmM dithiothreitol (DTT) , and all dilutions were made in lOmM DTT. Biotinylated KTSCVIM (seq id no:53) was synthesized on an Applied Biosystems 430A Synthesizer. The biotin group was added after removal of the N-terminal protecting group before cleavage of the peptide from the resin. Specifically, a 4-fold molar excess of biotin 4- nitrophenyl ester was added to the 0.5g resin in 75ml dimethylformanide at pH 8 and reacted for 5 hours at room temperature. Cleavage, identification, and purification were carried out as described above. To synthesize S-acetoamido CVIM (seq id no:10), purified CVIM was dissolved at a final concentration of ImM in 0.1ml of 0.5M Tris-chloride (pH 8.0) containing 15mM DTT. The tube was flushed with nitrogen for 2 min, sealed, and incubated for 2.5 hours at 37°C to reduce the cysteine residue, after which iodoacetamide was added to achieve a final concentration of 35mM. After incubation for 15min at 37°C, the reaction was stopped by addition of lOmMDTT. Complete alkylation of CVIM was confirmed by FAB spectrometry and HPLC. The molecular weight of the product corresponded to the expected molecular mass of S- acetoamido CVIM. b. Assay for Farnesyl:Protein Transferase The standard assay involved measuring the amount of [ 3 H] farnesyl transferred from all-trans [ 3 H] FPP to recombinant human p21 H"ras as described in Example I . Each reaction mixture contained the following concentrations of components in a final volume of 25μl: 50mM Tris- chloride (pH 7.5) , 50μM ZnCl 2 , 30mM KCl, ImM DTT, 30 or 40μM p21 H"ras , 15pmol [ 3 H] FPP (12-23,000 dpm/pmol) , 4 to 7.5μg of partially purified farnesyl :protein transferase (Mono Q fraction, see Example I) , and the indicated concentration of competitor peptide added in 3μl of lOmM DTT. After incubation for 30-60 min at 37°C, the amount of [ 3 H] farnesyl present in trichloroacetic acid- precipitable p21 H"ras was measured by a filter assay as described in Example I. A blank value (< 0.6% of input [ 3 H]FPP) was determined in parallel incubations containing no enzyme. This blank value was subtracted before calculating "% of control" values. c. Transfer of C^] Farnesyl from [ 3 H] FPP to Biotinylated KTSCVIM Peptide This assay takes advantage of the fact that peptides containing the Cys-AAX (seq id no:12) motif of ras proteins can serve as substrates for prenylation by farnesyl transferase. A heptapeptide containing the terminal four amino acids of p21 κ"rasB was chosen as a model substrate since it has a 20 to 40-fold higher affinity for the enzyme than does the COOH-terminal peptide corresponding to p21 H"ras . A biotinylated peptide is used as substrate so that the reaction product, [ 3 H] farnesylated peptide, can be trapped on a solid support such as streptavidinagarose. The bound [ 3 H] farnesylated peptide can then be washed, separated from unincorporated [ 3 H]FPP, and subjected to scintillation counting. The biotin-modified KTSCVIM (seq id no:53) is synthesized on an Applied Biosystems 430A Synthesizer using established procedures of solid phase peptide synthesis. The biotin group is added after deprotection of lysine and before cleavage of the peptide from the resin. The identity and purity of the biotinylated peptide is confirmed by quantitative amino acid analysis and fast atom bombardment (FAB) mass spectrometry. An aliquot of biotinylated KTSCVIM (seq id no:53; 0.4mg) is dissolved in 0.6ml of lOmM sodium acetate (pH 3) buffer containing ImM DTT and 50% ethanol to give a final concentration of 0.67mg/ml or 601μM. This solution can be stored at 4°C for at least 1 month. Immediately prior to use, the peptide solution is diluted with ImM DTT to achieve a peptide concentration of 18μM. The standard reaction mixture contains the following components in a final volume of 25μl: 50mM Tris-chloride (pH 7.5) , 50μM ZnCl 2 , 20mM KCl, ImM DTT, 0.2% (v/v) octyl- β-glucopryranoside, 10-15pmol of [ 3 H] FPP (15-50,000 dpm/pmol) , 3.6μM biotinylated KTSCVIM (seq id no:53) , and 2-4 units of enzyme. After incubation at 37°C for 30-60 min in 0.5-ml siliconized microfuge tubes, the reaction is stopped by addition of 200μl of 20mM Tris-chloride (pH 7.5) buffer containing 2 mg/ml bovine serum albumin, 2% SDS, and 150mM NaCl. A 25-μl aliquot of well mixed streptavidin-agarose (Bethesda Research Laboratories, Cat. No. 5942SA) is then added, and the mixture is gently shaken for 30 min at room temperature to allow maximal binding of the [ 3 H] farnesylated peptide to the beads. The beads are then collected by spinning the mixture for 1 min in a microfuge (12,500 rpm) . The supernatant is removed, and the beads are washed three times with 0.5ml of 20mM Tris-chloride (pH 7.5) buffer containing 2 mg/ml bovine serum albumin, 4% SDS, and 150mM NaCl. The pellet is resuspended in 50μl of the same buffer and transferred to a scintillation vial using a 200-μl pipettor in which the tip end has been cut off at an angle. The beads remaining in the tube are collected by rinsing the tube with 25μl of the above buffer and adding it plus the pipettor to the vial. A blank value, which consists of the radioactivity adhering to the beads in parallel incubations containing no enzyme, should be less than 0.5% of the input [ 3 H]FPP. 2. Results To screen peptides for their affinity for the farnesyl :protein transferase, studies were conducted wherein the ability of the peptides to compete with p21 H " ras for acceptance of t 3 H] farnesyl from [ 3 H] FPP as catalyzed by a partially purified rat brain farnesyl :protein transferase was tested. As a reference point for the peptides, the tetrapeptide CVIM (seq id no:10) corresponding to the COOH-terminal sequence of p21 κ"rasB was employed. Figure 10 shows a series of typical experiments in which alanine (Panel A) , lysine (Panel B) , or leucine (Panel C) was systematically substituted at each of the three positions following cysteine in CVIM (seq id no:10) . In each experiment the results were compared with those obtained with CVIM. Alanine and lysine were tolerated only at the Al position. Insertion of these amino acids at the A2 or X positions decreased the affinity for the enzyme by more than 30-fold as estimated by the concentration required for 50% inhibition. Leucine was tolerated at the A2 position, but it decreased the affinity when inserted at the X position. The substitution of phenylalanine for isoleucine at the A2 position increased the affinity for the enzyme by 6-fold, with half-maximal inhibition occurring at 25nM (Figure 11) . No such effect was observed when phenylalanine was inserted at either of the other two positions. In addition to performing assays with p21 H"ras as a substrate, assays were also performed in which the substrate was a biotinylated heptapeptide, KTSCVIM, which contains the COOH-terminal four amino acids of p21 H " rasB (Barbacid, 1987) . The biotin was attached to the NH 2 - terminus by coupling to the resin-attached peptide. The [ 3 H] farnesylated product was isolated by allowing it to bind to beads coated with streptavidin as described in section c. above. Figure 12 shows that the peptide CVFM (seq id no:34) was more potent than CVIM (seq id no:10) when either p21 H" ras or the biotinylated heptapeptide was used as acceptor (Panels A and B, respectively) . In contrast to the other studies, which were conducted with a partially purified enzyme, the studies of Figure 12 were carried out with a homogeneous preparation of affinity-purified farnesyl:protein transferase. The free sulfhydryl group for the cysteine is likely required for tetrapeptide inhibition, as indicted by the finding that derivitization with iodoacetamide abolished inhibitory activity (Figure 13A) . A blocked NH 2 -terminus is not required, as indicated by similar inhibitory activity of N-acetyl CVIM and N-octyl CVIM (Figure 13B) as compared to that of CVIM (Figure 13A) . Figure 14 summarizes the results of all competition assays in which substitutions in the CVIM sequence were made. The results are presented in terms of the peptide concentration required for 50% inhibition. Table III summarizes the results of other experiments in which tetrapeptides corresponding to the COOH-termini of 19 proteins were studied, many of which are known to be farnesylated. The implications of these studies are discussed below in Section 3. TABLE III INHIBITION OF RAT FARNESYLTRANSFERASE BY COOH-TERMINAL TETRAPEPTIDES CORRESPONDING TO KNOWN PROTEINS Protein Species COOH-Terminal Concentration Tetrapeptide for 50% Inhibition μM p21 K-rasB 0.15 p21 K-rasA 0.15 0.15 ι I 10 0.15 0.15 0.20 Retinal cGMP 0.35 phosphodies-terase, 15 n subunit rasl 0.35 * ras2 0.35 * γ-Subunit of 1.0 transducin 20 p21 H-ras Chicken CVIS 1.0 Protein Species I σi I 10 Enzyme activity was measured in the presence of the indicated tetrapeptide as described in the legend to Figure 10. Each tetrapeptide was tested at seven different concentrations ranging from 0.03 to lOOμM. The concentration giving 50% inhibition was calculated from the inhibition curve. * Shown to be farnesylated in vivo. 3. Discussion The current data extend the observations on the p21 ras farnesyl :protein transferase set forth in Example I, and further indicate that the recognition site for this enzyme is restricted to four amino acids of the Cys-Al- A2-X type. As a reference sequence for these studies, the peptide CVIM was used. This peptide inhibited the farnesyl:protein transferase by 50% at a concentration of 0.15μM. Substitution of various amino acids into this framework yielded peptides that gave 50% inhibitions at a spectrum of concentrations ranging from 0.025μM (CVFM; seq id no:34) to greater than 50μM (Figure 14) . In general, the highest inhibitory activities were achieved when the Al and A2 positions were occupied with nonpolar aliphatic or aromatic amino acids. This stringency was more severe at the A2 than at the Al position. Thus, peptides containing lysine or glutamic acid at the Al position gave 50% inhibition at 0.7 and 1.5μM, respectively. When these two residues were inserted at the A2 position, the affinity for the enzyme declined by more than 50-fold. Glycine and proline lowered inhibitory activity moderately at the Al position (50% inhibition at 4 and 8μM) and somewhat more severely at the A2 position (8 and 20μM) . The X position showed the highest stringency. In the context of CVIX (seq id no:56) , methionine was the preferred residue but phenylalanine and serine were tolerated with only modest losses in activity (0.5 and lμM, respectively) . Aliphatic resides and proline were disruptive at this position, with 50% inhibitions in the range of 5-llμM. Glutamic acid, lysine, and glycine were not tolerated at all; 50% inhibition required concentrations above 40μM. A study of tetrapeptides corresponding to the COOH- termini of known proteins (Table III) gave results that were generally in keeping with those obtained with the substituted CVIM (seq id no:10) peptides. They provided the additional information that glutamine and cysteine are well tolerated at the X position (CCVQ and CIIC; seq id nos:21 and 22) . All of the proteins that are known to be farnesylated in intact cells (indicated by the asterisks in Table III) followed the rules outlined above, and all inhibited farnesylation at relatively low concentrations (5μM or below) with the exception of the CTVA (seq id no:29) sequence, R . toruloides (Akada et al . , 1989) . This peptide inhibited the rat brain farnesyl:protein transferase by 50% only at the high concentrations of 30μM. It is likely that the farnesyl :protein transferase in this fungal species has a different specificity than that of the rat brain. The peptide CAIL (seq id no:65), which corresponds to the COOH-terminus of the 7-subunit of bovine brain G proteins (Gautam et al . , 1989; Robishaw et al . , 1989), did not compete efficiently with p21 H"ras for farnesylation (Table III) . A 50% inhibition at the highest concentration tested (lOOμM) was observed. The inhibitory activity was lower than that of CVIL (seq id no:26; 12μM) or CAIM (seq id no:14; 0.15μM) . Thus, the combination of alanine at the Al position and leucine at the X position is more detrimental than either single substitution. This finding is particularly relevant since the gamma subunit of G proteins from human brain (Yamane et al . , 1990) and rat PC12 cells (Mumby et al . , 1990) have been shown to contain a geranylgeranyl rather than a farnesyl. These findings suggest the existence of a separate geranylgeranyl transferase that favors CAIL (seq id no: 65) and perhaps other related sequences. The studies with the biotinyated heptapeptide (Figure 12B) confirm that at least some of the short peptides act as substrates for the enzyme. The saturation curves relating reaction velocity to the concentration of either p21 H"ras or the biotinylated heptapeptide are complex and sigmoidal. The inhibition curves with the various peptides differ from classic competitive inhibition curves. Finally, as mentioned in Example I, the maximal velocity of the purified enzyme is relatively low. These findings suggest that the binding of the peptides to the enzyme is not a simple equilibrium reaction. Rather, there may be a slow binding that requires conformational change. The observation that the Al position shows a relaxed amino acid specificity suggests that the residue at this position may not contact the farnesyl transferase directly. Rather, the contacts may involve only the cysteine and the residues at the A2 and X positions. A working model for the active site of the farnesyl:protein transferase places the peptide substrate in an extended conformation with a largely hydrophobic pocket of the enzyme interacting with the X group of the CAAX- containing substrate. EXAMPLE III RECOMBINANT CLONING OF THE RAT FARNESYL: PROTEIN TRANSFERASE o; AND S SUBUNIT cDNAs This example demonstrates the recombinant cloning of cDNAs corresponding to both the a and β subunit of rat farnesyl :protein transferase. The method employed by the inventors involved the application of the peptide sequence information, as detailed above, to prepare specific primers for PCR-based sequencing, which sequences were then used for the construction of probes with which to screen cDNA libraries. The cloning of each of these cDNAs by the inventors' laboratory has recently been reported (Chen et al . , 1991) . 1. Methods a. General Methods General molecular biological techniques were employed in connection with the cloning reactions described below, as set forth in Sambrook et al . , (1989) . cDNA clones were subcloned into bacteriophage M13 or plasmid pUC vectors and sequenced by the dideoxy chain termination method (Sanger et al . , 1977) using the M13 universal sequencing primer or gene specific internal primers. Sequencing reactions are preferably performed using a modified bacteriophage T7 DNA polymerase (Tabor et al . , 1987) with 35 S-labeled nucleotides, or Taq polymerase with fluorescently labeled nucleotides on an Applied Biosystems Model 370A DNA Sequencer. For the isolation of total cellular RNA from rat tissues, the inventors preferred to employ the guanidinium thiocyanate/CsCl centrifugation procedure (Glisin et al . , 1974) . Whereas for the isolation of RNA from cell lines, the guanidinium HCl method was found to be preferable (Chirgwin et al . , 1979) . The isolation of poly A + RNA by oligo(dT) -cellulose chromatography was achieved by the methods described in Sambrook et al . (1989) and Aviv et al . (1972) . Northern blot hybridization using single-stranded 32 P-labeled probes was carried out as described by Lehrman et al . (1987) . A cDNA probe for rat glyceraldehyde-3-phosphate dehydrogenase was obtained from Karl Normington, (University of Texas Southwestern Medical Center at Dallas) . Polyclonal antisera, specific for either the cn or β subunit of farnesyl transferase, were prepared by immunizing rabbits with synthetic peptides derived from each specific subunit. Antibody Y533 was raised against a synthetic peptide with the sequence LQSKHSRESDIPASV (seq id no:67) , derived from the predicted amino acid sequence of a cDNA clone of the a subunit. Antibody X287 was raised using the synthetic peptide IQATTHFLQKPVPGFEE (seq id no:68), derived from a tryptic digest of the β subunit. Each peptide was coupled to Keyhole Limpet hemocyanin using maleimidobenzoic acid N-hydrosuccinimide ester (Signa Chemical Co.) (Harlow & Lane 1988) . For each antibody, three New Zealand White rabbits were immunized with 600μg of coupled peptide in Freund's complete adjuvant. Immunoblot analysis was performed as described in (Seabra et al . , 1991; Chen et al . , 1991) . Rat PC12 pheochromocytoma cells, rat KNRK cells (CRL 1569) , and human embryonic kidney 293 cells were obtained, respectively, from Thomas Sύdhof (University of Texas Southwestern Medical Center at Dallas) , the American Type Culture Collection, and Arnold J. Berk (University of California, Los Angeles) . b. PCR and Probe synthesis To derive a sequence for constructing an appropriate probe, rat genomic DNA may be used as a template for PCR as described by Saiki et al . (1988) and Lee et al . (1988) . The approach used by the inventors was to sequence a portion of the cn or β subunit genes through the use of appropriate PCR primers, based on a consideration of the peptide sequences (shown in Table I) . Thus, PCR was used to obtain the rat genomic DNA sequences that encoded tryptic peptides derived from either the purified o; or β subunits of rat farnesyl transferase (Figure 16) . For the both the cn and β sequences, the PCR primers were synthesized based on the NH 2 - and COOH-terminal sequences of the peptides shown in Figure 16, and included the degenerate inosine codons indicated (Figure 16) . PCR primers were end-labeled with [7~ 32 P]ATP. Each of the amplified DNA fragments were eluted from 12% acrylamide gels and sequenced by the method of Maxam and Gilbert (Maxam et al . , 1980) . Translation of the nucleotide sequences between the two primers yielded peptides with amino acid sequences identical to those of the peptides shown (Figure 16) . Using the DNA sequences of the PCR products, the inventors then synthesized an oligonucleotide probe that would hybridize with the region corresponding to the peptide, for use in the direct screening of the library. For the cn subunit, a 38-mer probe with the nucleotide sequence: 5'-ATIGAGTTAAACGCAGCCAACTATACGGTCTGGCACTT-3', (a specific example in accordance with residues 6-54 of seq id no:64) , was synthesized. Whereas for the β subunit, two primers, designated primer β3 and primer β4 were synthesized with the respective nucleotide sequences: 5"-GCGTACTGTGCGGCCTC-3' (residues 1-17 of seq id no:62) and 5'-GGCCTCAGTAGCCTCTCTCACCAAC-3' (residues 12-36 of seq id no:62) . The primers for the β subunit were used for 3'-end amplification of cDNA as described by Frohman et al . (1988) . Poly(A) + RNA from rat KNRK cells was reverse transcribed using a (dT) 17 -adaptor, 5'-GACTCGAGTCGACATCGA(T) 17 -3' (seq id no:69) . The 50μl reaction mixture, containing 4μg poly(A) + RNA, 2.5μg (dT) π -adaptor, and 100 units of Moloney murine leukemia virus reverse transcriptase (Bethesda Research Laboratories) , was incubated at 37°C for 1 hour. Reverse transcribed cDNA was diluted 50-fold with lOmM Tris-HCI at pH 8.0, ImM EDTA, and subjected to specific PCR amplification as follows. lOμl of diluted cDNA, 25pmol of adaptor primer (5'-GACTCGAGTCGACATCG-3' ; residues 1-17 of seq id no:69) , and 25pmol of primer 3 were boiled, after which PCR was carried out for 40 cycles (95°C, 40 sec; 58°C, 1 min; 72°C, 3 min) with TaqI polymerase. Amplified PCR products were subjected to electrophoresis on an agarose gel, transferred to a nylon membrane, and probed with 32 P-labeled primer 4. The hybridizing DNA fragment was eluted, extracted with phenol/chloroform, and used as a template for a second PCR reaction. The reaction using 25 pmol each of adaptor primer and primer 4 was carried out with the same amplification protocol as described above. The reamplified DNA fragment was gel- purified, cleaved with Rsal or TaqI, and subcloned into an M13 vector for DNA sequencing and for subsequent generation of the single-stranded M13 probe that is referred to as Probe B. The DNA sequence of the PCR- derived clone was also used to generate a 50-mer oligonucleotide probe that is designated Probe A. Probes A and B were then used to screen cDNA libraries in order to obtain a full-length β subunit cDNA (see β subunit cloning section, below) . c. cDNA Libraries and Cloning Rat PC12 cell poly(A + ) RNA and oligo (dT) -primed KNRK cell double-stranded cDNA libraries were constructed in bacteriophage λgtlO, using a cDNA synthesis kit from Invitrogen. These cells were preferred because the inventors believed them to be rich in farnesyl :protein transferase mRNA. Although numerous convenient methods are known for the construction of cDNA libraries, the inventors utilized the above kit from Invitrogen as they thought it to be a particularly convenient method. The cDNA itself was prepared using both oligo (dT)- and random hexamer-primed cDNA, then ligated to a suitable linker, with the EcoRl/Notl linker being preferred in this case. cDNAs larger than 1 kb were isolated by size fractionation using a 1% agarose gel and ligated into EcoRl-cleaved λgtlO DNA (Stratagene) , in order to complete the construction of the cDNA-containing vectors for library preparation. After in vi tro packaging of the recombinant lambda phage with a DNA packaging extract (Stratagene) , phage were plated out on host strain E. coli C600 hfl " cells. a subunit cloning. Approximately 1 x IO 6 plaques of the rat brain library were screened. Duplicate filters were hybridized in 6xSSC (lxSSC= 150mM NaCl/l5mM Na citrate, at pH 7.0) with 1 x IO 6 cpm/ml of 32 P-labeled probe (see above) . One positive clone, λRB-17, with an insert of 1.4 kb was identified and plaque purified. Phage DNA from a plate lysate was subcloned into bacteriophage M13 and pBluescript vectors for DNA restriction mapping and sequencing (Sanger et al . , 1980) . As the clone first obtained was not a full-length clone, 5'-end amplification was employed to produce the complete sequence, as described in Ref 34. Firstly, an M13 probe corresponding to the 5' end of λRB-17 was used to screen the KNRK cell library. Replicate filters were hybridized in 50% (v/v) formamide containing 1 x IO 6 cpm/ml of the probe. Positive clones were analyzed by PCR, and the clone with the longest insert (λKNRK-3) was purified and subcloned for analysis. A 5' Rapid Amplification of cDNA End procedure (5' RACE) (34) was used to extend the 5' end of λKNRK-3. An M13 probe derived from the amplification product (RACE-5') was then used to screen a rat testis library (purchased from Clontech) , yielding λRTH, which extended to nucleotide position 53. To obtain the extreme 5' end of the cDNA, a primer- extension λgtlO library was constructed from rat testis poly(A) + RNA. First stand synthesis was primed with an oligonucleotide corresponding to a sequence near the 5'- end of RACE-5' using Maloney murine leukemia virus reverse transcriptase. After incubation at 37°C for 1 h, the reaction was heated at 70°C for 5 min. Five units of Thermostable rTth Transcriptase (Perkin-Elmer) was then added, and the reaction continued at 70°C for 30 min. After second strand synthesis, the cDNAs were ligated to an EcoRI/NotI linker. Excess linkers were removed by Centricon 100 Microconcentrator (Amicon) . Approximately 5 x 10 5 plaques were screened with a 32 P-labeled probe corresponding to nucleotides 54-104, which was obtained from the sequence of λRTH. Twenty-five positive clones were identified. Phage DNA was prepared from plate lysates, and the insert from one of the longest clones, λPE-7, was subcloned for sequencing (Sanger et al . , 1980) . β subunit cloning. Approximately 5 x 10 5 plaques were transferred to replicate filters. One filter was hybridized in 10% (v/v) formamide with 1 x IO 6 cpm/ml of a 32 P-labeled 50-mer oligonucleotide probe (Probe A; described above) . The other filter was hybridized in 50% formamide with 1 x IO 6 cpm/ml of a single-stranded M13 probe (Probe B; described above) . One positive clone (λdT-7) with an insert of -2.3 kb was identified with both probes and plaque purified. Phage DNA isolated from the plate lysate of λdT-7 was subcloned into M13 and pUC vectors for sequencing and restriction mapping. To obtain the extreme 5' end of the cDNA, an M13 probe corresponding to the 5' end of λdT-7 was used to screen a rat brain "5'-stretch" cDNA library (purchased from Clontech) . Replicate filters were hybridized in 50% formamide containing 1 x IO 6 cpm/ml of the probe. Of the 5 x IO 5 plaques screened, six positive clones were plaque purified and eluted in 0.2ml buffer containing lOOmM NaCl, 8mM MgS0 4 , 50mM Tris-HCI at pH 7.5, and 0.01% (w/v) gelatin. A primer corresponding to the right arm or left arm of λgtlO sequences flanking the unique EcoRl cloning site was used in combination with a primer derived from the 5' end of the rat protein farnesyl transferase cDNA (λdT-7) for a PCR reaction. PCR products were analyzed on an agarose gel, and the clone containing the longest extension, λRB-23, was subcloned for further analysis. d. Expression Vectors Expression vectors for the cn subunit of rat farnesyl transferase were constructed in pCMV5, a plasmid that contains the promoter-enhancer region of the major immediate early gene of human cytomegalovirus (Andersson et al . , 1989) . A PvuII fragment containing 20 base pairs of the 5' untranslated region and the entire coding region was excised from clone λRTH-B and ligated into Smal-digested pCMV5 in both orientations. Plasmid λRTH-B is identical to λRTH except for the presence of an intron in the 5'-untranslated region at nucleotide position 39, upstream of the PvuII site at position 37-42. The resulting piasmids designated pFT-α_ (correct orientation) and pFT-otrev (reverse orientation) , were characterized by restriction mapping. Expression vectors for the β-subunit of rat farnesyl transferase were also constructed in pCMV5 (Andersson et al . , 1989) . An EcoRl fragment containing the entire 5' untranslated region and the coding region of farnesyl transferase β subunit cDNA was excised from clone λRB-23 and ligated into EcoRl-digested pCMV5 in both orientations. The resulting piasmids, designated pFT-3l (correct orientation) and pFT-βlrev (reverse orientation) , were characterized by restriction mapping. e. DNA Transfection Human embryonic kidney 293 cells were grown in monolayer at 37°C in medium A (Dulbecco's modified Eagle medium supplemented with 10% (v/v) fetal calf serum, 100 units/ml of penicillin, and 100 μg/ml streptomycin) . On day 0, 6 x 10 5 cells/lOO-mm dish were seeded in medium A. On day 1, each dish of cells was transfected with 3μg of the indicated plasmid plus lμg of pVA (a plasmid encoding adenovirus VA RNA I; - Akusja et al. , 1987) by the calcium phosphate method (Sambrook et al., 1989) . On day 2, the cells received fresh medium A. On day 4, the cells from two dishes were harvested, pooled, and disrupted by repeated aspiration at 4°C through a 25-gauge needle in 0.4 ml buffer containing 50mM Tris-HCI at pH 7.5, 50μM ZnCl 2 , 3mM MgCl 2 , 20mM KCl, ImM dithiothreitol, and 0.4% (w/v) octyl-/3-glucopyranoside. A cytosolic extract was obtained by centrifugation at 100,000 x g for 1 h at 4°C, after which 0.16 to 5.4μg of the supernatant fraction were assayed for farnesyl transferase activity by measuring the amount of [ 3 H] farnesyl transferred from [ 3 H] farnesyl pyrophosphate to p21 H"ras protein as described above. 2. Results a. o> subunit Cloning and Sequence Analysis Degenerate oligonucleotide probes encoding the 5' and 3' ends of a tryptic peptide derived from the farnesyl transferase cn subunit were used as primers in a PCR employing rat genomic DNA (Figure 16A) . The sequence of the amplified product was used as a probe to screen a random hexanucleotide-primed rat brain cDNA library cloned in λgtlO. This procedure yielded λRB-17, which extended from a poly A tract up to nucleotide position 345 (this position refers to the final sequence of the mRNA, as in seq id no:2) . The 5'-end of the mRNA encoding the α subunit was found to contain a sequence extremely rich in GC basepairs (76% GC from nucleotides 71 to 205 and 90% GC from nucleotides 116 to 145) . Multiple attempts to traverse this region by primer extension using reverse transcriptase gave products that terminated prematurely, or that encoded unspliced introns. Therefore, other strategies were employed in order to obtain the 5'-end of the mRNA (see above methods section for detailed protocols) . A composite of the cDNA sequences thus obtained was used to generate the overall sequence of the mRNA (seq id no:2) . The mRNA was found to encode a protein of 377 amino acids (seq id no:l) with a calculated molecular weight of 44053. Although the cDNA sequence did not contain a terminator codon upstream of the first methionine codon, it is believed that this methionine represented the true initiator codon. This is supported by transfection studies, in which the recombinant protein produced was observed to have a molecular weight that was indistinguishable on immunoblots from that of the purified rat brain cn subunit (see below and Figure 20) . If the cDNA were incomplete, the initiator methionine must be upstream of the 5' end of the sequence obtained, and therefore the protein produced by the cDNA should be at least 2 kDa smaller than the authentic protein. Such a difference should have been detected in gel electrophoresis experiments. The most remarkable feature of the cn subunit cDNA was determined to be a string of 9 consecutive proline residues near the NH 2 -terminus (in seq id no:2) , whose codons accounted for much of the extreme GC-richness of this region. The mRNA contained sequences corresponding to sequences of the peptides obtained following tryptic digestion of the purified subunit . Discrepancies only occurred at positions that were assigned tentatively in sequencing trace amounts of protein (see Table I) . Some slight homology has been noted between the rat cn subunit amino acid sequence and yeast RAM2, the sequence of which is reported in He et al . (1991) . The residues of the rat α subunit amino acid sequence (seq id no:l) which are identical to those of the yeast RAM2 sequence are boxed in Figure 17. Recently, Kohl et al . have reported the cloning of a partial cDNA clone corresponding to the bovine ex subunit of farnesyl transferase (Kohl et al . , 1991) . The 329 amino acids encoded by this partial clone are 95% identical to the corresponding region in the cn subunit of the rat farnesyl transferase. Comparison of the complete amino acid sequence of rat farnesyl transferase cn subunit (377 amino acids) with that of the yeast RAM2 gene product (316 amino acids) disclosed by He et al . (1991) reveals that the two proteins are 39% identical in the COOH-terminal 211 residues, suggesting that RAM2 is the yeast counterpart of the cn subunit of mammalian farnesyl transferase. b. β subunit Cloning and Analysis A unique DNA sequence encoding a portion of the β subunit of the rat farnesyl transferase was obtained by the polymerase chain reaction (PCR) with rat genomic DNA and degenerate oligonucleotide primers (primers βl and β2; seq id no:60 and 61, respectively) corresponding to potential sequences encoding a tryptic peptide obtained from the purified rat brain enzyme (Figure 16B) . Two unique oligonucleotides (primers β3 and β4, residues 1-17 and 12-36 of seq id no:62, respectively) were synthesized based on the sequence of the amplified product (Figure 16B) . These primers were then used in a 3'-end amplification strategy (Frohman et al . , 1988) to obtain an amplified fragment from cDNA prepared from mRNA isolated from cultured rat kidney cells (KNRK cells) . This fragment was used to generate probes that identified a bacteriophage containing a near full-length cDNA (λdT- 7) from a cDNA library prepared from rat pheochromocytoma PC12 cells. Finally, a fragment from the 5'-end of λdT-7 was used to identify a clone containing a full-length farnesyl transferase β subunit cDNA (λRB-23) from a rat brain cDNA library (seq id no:4) . The cDNA for the rat brain farnesyl transferase β subunit contains 59 base pairs of 5'untranslated region followed by protein-coding region of 1314 base pairs and a 3'untranslated region of 1091 base pairs (seq id no:4) . The cDNA encoded a protein of 437 amino acids (seq id no:3) and contained sequences corresponding to sequences of the peptides obtained following tryptic digestion of the purified rat brain farnesyl transferase β subunit. Although certain minor discrepancies in sequence between the protein and the cDNA were apparent, these occurred near the COOH-termini of the peptides and were attributed to ambiguities in sequencing the trace amounts of peptide that were available (see Table I) . The cDNA clones did not contain an inframe terminator codon upstream of the first methionine (amino acid residue 1 in seq id no:3) . This is believed to be the initiator methionine as it lies in a good context for initiation according to Kozak's rules (Kozak, 1984) and because the cDNA encodes a protein of the same size as the jS-subunit when transfected into animal cells (see below) . Although λdT-7 was obtained from an oligo-dT primed cDNA library, the clone did not contain a poly A tract, nor did it contain a consensus polyadenylation sequence. However, RNA blot hybridization experiments and expression studies (see below) suggested that the clone is essentially full-length. The molecular weight of the β subunit of the rat brain farnesyl transferase was calculated to be 48,679. The amino acid composition did not show any particularly remarkable features and the calculated isoelectric point was 5.99. An analysis of the hydrophobicity plots did not reveal any extensive hydrophobic sequences. A search of the GenBank and EMBL data banks revealed significant resemblance to two proteins, the DPR1-RAM1 protein of yeast Saccharomyces cerevisiae and a yeast open reading frame of unidentified function (0RF2) . Extensive stretches of identity were evident between the β subunit protein sequence and the yeast DPRl-RAMl gene product (Figure 18) . Sequence conservation was observed throughout the two proteins (overall identity: 37%) , but was found to lessen at both ends, and the yeast protein was shorter by six amino acids. The residues of the rat β subunit amino acid sequence (seq id no:3) which are identical to those of the yeast DPRl-RAMl sequence are boxed in Figure 18. In an article by Kohl et al . (1991) , in a note added in proof, it is indicated that the β-subunit of bovine farnesyl transferase has been cloned and that it shares 96% homology to the rat /3-subunit. However, no actual sequences corresponding to the β-subunit are disclosed by Kohl et al . (1991) . c. Northern Blotting Analyses Northern RNA blot analysis with 32 P-labelled probes derived from the cn subunit cDNA revealed a single mRNA of -1.75 kb in multiple rat tissues, including lung, heart, kidney, brain, adrenal, and testis (Figure 19A) . The amount of mRNA in testis was several-fold higher than in any other tissue, an observation that was repeated on several occasions. An mRNA of the same size was also observed in two lines of cultured rat cells derived from kidney (KNRK cells) and adrenal medulla (PC12 cells) (Figure 19B) . Northern RNA blot analysis with 32 P-labelled probes derived from the β subunit cDNA revealed a hybridizing mRNA of -2.5 kb in all rat tissues examined except liver and spleen (Figure 19C) . Adequate amounts of mRNA from these tissues were applied to the filter as confirmed by hybridization with control probes for cyclophilin and glyceraldehyde-3-phosphate dehydrogenase. The brain and adrenal gland appeared to have somewhat more mRNA for farnesyl transferase β-subunit than did the other tissues. More quantitative studies will be required to determine whether the variations shown in Figure 19C are significant. The MRNA for the farnesyl transferase jS-subunit was also found in the two cultured rat cell lines from which CDNA sequences had been obtained (Figure 19D) . PC12 cells had the 2.5-kb transcript, whereas the KNRK cells had two transcripts, one of which was smaller than the 2.5-kb MRNA (Figure 19D) . It was not determined whether the smaller transcript represented another gene product that cross-hybridized with the jS-subunit probe, or whether this MRNA represented alternative processing of an allelic transcript. d. Co-Expression and Stability The cDNA coding regions of both the cn and β subunits were cloned into pCMV mammalian expression vectors in either the correct or the reverse orientation. The cDNAs were introduced into human kidney 293 cells by calcium phosphate-mediated transfection, and the proteins were detected by immunoblotting with specific antibodies against the cn and β subunits. In both cases, the cDNA directed the expression of proteins with molecular weights that were indistinguishable on immunoblots from those of the purified rat brain farnesyl transferase oι and β subunits (Figure 20) . The accumulation of detectable amounts of a subunit required simultaneous transfection with a properly oriented cDNA encoding the β-subunit (Figure 20A) . Similarly, the amount of detectable 0-subunit was increased by transfection with the subunit cDNA in the correct orientation (Figure 20B) . Transfection with the two cDNAs in the correct orientation was also required in order to produce significant amounts of p21 ras farnesyl transferase activity as measured in cytosolic extracts (Figure 21) . 3. Discussion The delineation of the amino acid sequence of the α. subunit has not yet allowed its catalytic role to be precisely identified. Homology searches of protein databases failed to reveal significant resemblance of the a subunit to other proteins except for proteins that contain long stretches of prolines. These include such apparently unrelated proteins as the catalytic subunits of rat and human protein phosphatase 2B, mouse retinoblastoma-associated protein ppl05, and Dictyostelium discoideum protein tyrosine kinase-1. The cn subunit does not bear significant structural resemblance to known prenyltransferases such as mammalian farnesyl pyrophosphate synthetase or yeast hexaprenyl pyrophosphate synthetase. Present evidence suggests that the a subunit may be shared with another prenyltransferase with a different β subunit that exhibits geranylgeranyltransferase activity (Seabra et al . , 1991) . If the shared cn subunit is stable only as a complex with one of several β subunits, this mechanism would assure that cells maintain only enough cn subunits to satisfy all of the β subunits, thereby avoiding accumulation of excess α. subunits, which might be toxic (Chen et al . , 1991) . The above data reveal that the α and β subunits of the rat farnesyl transferase do not exhibit farnesyl transferase activity when expressed by themselves in transfected human 293 cells. However, co-expression of the two subunits results in the production of an active enzyme. Such expression data provides support for the previous conclusion that the farnesyltransferase is a heterodimer that requires both the α and β subunits for catalytic activity (Chen et al . , 1991) . Furthermore, the transfection experiments indicate that mammalian cells will not accumulate high levels of either subunit of the farnesyltransferase unless the other subunit is present. This is particularly true for the cn subunit, whose accumulation was nearly completely dependent on co-expression of the β subunit. It is likely that the cn subunit is rapidly degraded unless the β subunit is present. However, until pulse-chase labeling experiments are performed, the possibility of control at the level of mRNA stability or translation cannot be ruled out . The similarity between the rat β subunit and the previously reported sequence of the yeast DPRl -RAMl gene product (Goodman et al . , 1990) indicates that the latter is the yeast equivalent of the peptide-binding subunit of the mammalian farnesyl transferase. These findings confirm the previous suspicion that the yeast gene encodes one of the subunits of the farnesyl transferase and explains the failure of this protein to exhibit farnesyl transferase activity when expressed alone in E. coli (Goodman et al . , 1988; Schafer et al . , 1990) . Mutations at a second locus, designated RAM2 , also disrupt farnesyl transferase activity in yeast (Goodman et al . , 1990) . The defect in the RAM2 cells is complemented by mating with the DPRl -RAMl mutant. This finding suggests that the RAM2 gene product is the cn subunit of the yeast farnesyl transferase. A more recent report of He et al . (1991) indicates that coexpression of the RAM1 and RAM2 genes in E. coli provided extracts that farnesylated synthetic a-factor substrate. However, when extracts from separate clones were mixed, only partial farnesyl transferase activity, on the order of about 3.5%, was recovered. An inspection of the conserved sequences in the rat β subunit and the DPRl-RAMl protein fails to reveal any obvious candidates for the peptide binding site. The rat protein (residues 35-41) does contain the sequence L.XDDXXE (seq id no:70) , which resembles a sequence in four polyprenyl synthetases in which lie, Leu or Val precedes the XDDXXD sequence (residues 2-7 of seq id no:70) that is believed to be a prenyl pyrophosphate binding site (Ashby and Edwards, 1990) . This sequence is not found in the same position in the DPRl-RAMl protein, and its significance in the β subunit is uncertain. Although the farnesyl transferase reaction requires two divalent cations (Mg ++ and Zn ++ ) , the sequence of the β subunit does not reveal any obvious metal binding sites . Recently, the inventors have explored the separate catalytic roles of Zn 2+ and Mg 2+ and the specificity of the prenyl pyrophosphate binding site of the rat brain protein farnesyltransferase, using a purified enzyme preparation. In summary, it was found that the binding of p21 H"ras to the enzyme was abolished by dialysis against EDTA and restored by addition of ZnCl 2 as demonstrated by chemical crosslinking. The binding of the other substrate, all- trans farnesyl pyrophosphate, was independent of divalent cations, as demonstrated by gel filtration. Transfer of the enzyme-bound farnesyl group to the bound p21 H"ras required Mg 2+ . Geranylgeranyl pyrophosphate bound to the prenyl pyrophosphate binding site with an affinity equal to that of farnesyl pyrophosphate, but the geranylgeranyl group was not transferred efficiently to P21 H"ras . It also was not transferred to a modified p21 H"ras containing COOH-terminal leucine, a protein that was shown previously to be a good substrate for a rat brain geranylgeranyltransferase (Seabra et al . , 1991) . The inventors conclude that the protein farnesyltransferase is a metalloenzyme that most likely contains Zn 2+ at the peptide-binding site. It thus resembles certain metallopeptidases, including carboxy- peptidase A and the angiotensin-converting enzyme. Strategies previously developed to screen for inhibitors of those enzymes will likely aid in the search for inhibitors of the protein farnesyltransferase. Thus, these data establish several new points about the enzymology of the protein farnesyltransferase from rat brain: 1) the enzyme contains a tightly bound divalent cation, most likely Zn 2+ , that can be removed by dialysis against EDTA; 2) Zn 2+ is essential for binding of the peptide substrate, and therefore it is probably attached to the β-subunit; 3) the enzyme binds FPP and GGPP with comparable affinities, but transfers only the farnesyl group and only to an acceptor whose CaaX sequence ends in methionine, serine, glutamine, or cysteine, but not leucine; 4) binding of prenyl pyrophosphates does not require any cation; and 5) transfer of the bound farnesyl group to the bound peptide acceptor requires Mg 2+ . The reaction sequence for the EDTA-treated protein farnesyltransferase is summarized graphically in Figure 22. The EDTA-treated enzyme binds FPP without a requirement for prior Zn 2+ binding. Peptide binding requires Zn 2+ , but is independent of FPP binding. After both substrates are bound, the transfer reaction occurs in a Mg 2+ - dependent fashion. In the cell the enzyme is expected to be constitutively complexed with Zn 2+ . Under these conditions the mechanism is a simple random- ordered, two-substrate reaction in which the FPP and peptide acceptor can bind to the enzyme in any order. The requirement for Zn 2+ in peptide binding is reminiscent of the requirement for Zn 2+ in certain metallopeptidases, such as carboxypeptidase A (Lipscomb, 1974) . In this case the Zn 2+ coordinates with the carbonyl and amino groups in the peptide bond that will be broken. In the farnesyltransferase the Zn 2+ is likely to coordinate with the cysteine sulfhydryl group on the acceptor peptide. If this postulated mechanism is correct, inhibitors that mimic peptides that coordinate with Zn 2+ might be effective inhibitors of the farnesyltransferase. This strategy would be very similar to the strategy followed in the design of inhibitors of the angiotensin-converting enzyme, a zinc metalloenzyme that is mechanistically similar to carboxypeptidase A (Petrillo and Ondetti, 1982) . The ability of GGPP to compete with FPP for the prenyl pyrophosphate binding site on the protein farnesyl-transferase creates potential regulatory problems for the cell. If the mtracellular concentrations of FPP and GGPP are similar, then the farnesyltransferase might be competitively inhibited at all times. It seems likely that the concentration of GGPP in the cell is lower than that of FPP. FPP is an intermediate in the synthesis of cholesterol, which is the bulk product of the pathway (Goldstein and Brown, 1990) . GGPP, on the other hand, is not known to be converted into any other metabolites in animal cells, and indeed its existence in animal cells was not appreciated prior to the discovery of geranylgeranyl-modified proteins (Farnsworth et al. , 1990; Rilling et al. , 1990) . Thus, it seems likely that cells avoid GGPP competition by maintaining the FPP concentration at a higher level than the GGPP concentration. If the cn subunit is involved in prenyl phrophosphate binding and if the cn subunit of the farnesyltransferase is identical to that of the leucine-recognizing geranyl¬ geranyltransferase, then the cn subunit must behave differently when it is part of the geranylgeranly- transferase. It seems unlikely that the geranylgeranyl¬ transferase would be inhibited by FPP because this would render the enzyme functionally inactive in the cell. Resolution of this issue will require the purification of the leucine-recognizing geranylgeranyltransferase and the determination as to whether its cn subunit is identical to, or merely similar to, the α; subunit of the farnesyltransferase. The binding of prenyl pyrophosphates to the farnesyltransferase appears to be independent of divalent cations. In this regard the farnesyltransferase resembles the prenyltransferase that catalyzes the condensation of isopentenyl pyrophosphate with allylic pyrophosphates to form FPP (King and Rilling, 1977) . The two enzymes also resemble each other in the requirement for a divalent cation (Mg 2+ or Mn 2+ ) in the transfer reaction. In studies not shown, it was found that Mn 2+ will substitute for Mg 2+ in the protein farnesyltransferase reaction. The two enzymes differ in that the FPP synthetase is a homodimer and it shows no requirement for Zn 2+ (Rilling, 1985) . Turning to the issue of the yeast counterpart prenyl transferases, very recently two additional putative β subunits of yeast prenyltransferases have been identified, BET2 (Rossi et al . , 1991) and CALl (Ohya et al . , 1991) . Both sequences resemble the DPR1/RAM1 gene product and the β subunit of the rat brain farnesyl transferase. A mutation in the BET2 gene prevents the membrane attachment of two small GTP binding proteins (YPT1 and SEC4) that direct vesicular traffic in the yeast secretory pathway (Rossi et al . , 1991) . These proteins terminate in the sequence CC, which has recently been shown to be geranylgeranylated in animal cells (Khosravi-Far et al . , 1991) . The second putative β- subunit, encoded by the CALl gene, is necessary for yeast to control the cell cycle when deprived of calcium. Based on a genetic argument, it has been suggested that the targets for this prenyltransferase are two proteins that end in a Cys-X-X-Leu (seq id no:71) sequence and are believed to be geranylgeranylated (Ohya et al . , 1991) . Considered together, the yeast and animal experiments suggest the existence of a family of closely related β subunits that mediate peptide binding to a variety of prenyltransferases. Whether all of these enzymes have the same cn subunit, or whether a family of such subunits also exists, remains to be determined. EXAMPLE IV RECOMBINANT CLONING OF THE HUM&N FARNESYL: PROTEIN TRANSFERASE α AND β SUBUNIT cDNAs The inventors have now succeeded in cloning cDNAs encoding both the cn and β subunits of the human farnesyl :protein transferase. This was carried out using molecular cloning techniques with the aid of the information gained from the rat farnesyl :protein transferase gene disclosed herein. 1. subunit Cloning and Sequence Analysis Approximately 1 x IO 6 plaques from a human retinal λgtlO cDNA library (obtained from Jeremy Nathans, Johns Hopkins University Medical School, Baltimore, MD) were screened using 32 P-labeled probes corresponding to the 5' end of the cDNA for the rat farnesyl transferase a; subunit, as disclosed herein and in Chen et al . , (1991a) . Filters were hybridized at 42°C in hybridization buffer with 50% (v/v) formamide containing 1 x IO 6 cpm/ml of a single-stranded M13 probe and washed in IXSSC (150 mM sodium chloride and 15 mM sodium citrate, pH7) and 0.5% (w/v) SDS at 55°C. On screening the human retinal cDNA library with 32 P- labeled probes derived from the rat cn subunit cDNA, several positive clones were identified. These were initially characterized by polymerase chain reaction (PCR) using primers corresponding to the right and left arms of λgtlO. Positive clones containing the largest inserts were plaque purified, phage DNA prepared, and the cDNA inserts subcloned into the Bluescript (Stratagene) SKII vector for restriction mapping and DNA sequencing (Sanger et al . , 1980) using specific oligonucleotides. The nucleotide sequence of the human farnesyltransferase cn subunit, as encoded by the cloned cDNA, is represented by seq id no:6. This coding region is followed by a 3' -untranslated region of 524 nucleotides that ends in a poly(A) tail. The cloned cDNA encodes a human cn subunit protein of 379 amino acids, represented by seq id no:5, which is two amino acids longer than the deduced rat sequence (Figure 26) . Overall, the human farnesyltransferase cn subunit is 93% identical to the rat cn subunit at the protein level (Figure 26) . In the coding region, the nucleotide sequence of the human cDNA is 79% identical to that of the rat. When introduced together into the human kidney 293 cell line by transfection, the human farnesyltransferase cn subunit cDNA and the rat farnesyltransferase β subunit cDNA produced an active enzyme, as was the case when the cDNAs encoding both of the rat subunits were co-transfected into 293 cells (disclosed herein) . 2. β subunit Cloning and Sequence Analysis PCR was used to produce a probe specific for the human farnesyltransferase β subunit . Human prostate poly (A) + RNA was subjected to first strand synthesis (Chen et al . 1991a; 1991b), and then used as a template in a PCR reaction with a primer pair developed from the rat farnesyl transferase β subunit, as disclosed herein and in Chen et al . , (1991b) . The 300 bp amplified product was sequenced and shown to correspond to the human farnesyl transferase β subunit. On screening 1.5 x IO 6 plaques from the human retinal λgtlO cDNA library with the 32 P-labeled probe corresponding to the PCR-product, 9 positive clones were identified. Positive clones containing the largest inserts were plaque purified, phage DNA prepared, and the cDNA inserts subcloned into M13 and pUC18 vector for restriction mapping and DNA sequencing (Sanger et al . , 1980) using the M13 universal sequencing primer. The nucleotide sequence of the human farnesyltransferase β subunit, as encoded by the partial cDNA clone obtained, is represented by seq id no:8. This partial cDNA clone encodes a human β subunit protein of 487 amino acids (seq id no:7) , 50 amino acids shorter than the deduced rat sequence (Figure 27) . Overall, the human farnesyltransferase β subunit is 96% identical to the rat farnesyltransferase β subunit at the protein level (Figure 27) . In the coding region, the nucleotide sequence of the human cDNA (seq id no:8) is 87% identical to the rat sequence (seq id no:4) . 3. Discussion In a disease or disorder where the function of CAAX farnesyl transferase and the related prenyltransferase, CAAX geranylgeranyl transferase, is potentially important, an abnormality of either the or β subunit of CAAX " farnesyltransferase or CAAX " geranylgeranyl transferase might either cause or exacerbate the condition. It would appear that mutations in either the cn subunit or the β subunit of farnesyltransferase would have pleiotropic effects because of the number of different proteins and systems that are affected by prenylation. Pleiotropy would be expected to be particularly evident in mutations that affect the farnesyltransferase a subunit since this protein is the cn subunit for both the CAAX farnesyltransferase and CAAX geranylgeranyl transferase. Different mutations in critical regions of the cn or β subunits of farnesyltransferase may have a differential effect on individual GTP-binding proteins. For p21 ras proteins, farnesylation assists attachment of p21 ras to the inner surface of the plasma membrane. It is believed that farnesylation increases the efficiency with which oncogenic ras proteins stimulate cell growth. It is possible that amplification or activating mutations of either the α. or β subunits of the farnesyltransferase enzyme may affect tumor cell growth and progression indirectly by increasing the attachment efficiency of p2l ras proteins. EXAMPLE V NH,-TERMINAL POSITIVE CHARGE CREATE PURE INHIBITORS The examples above demonstrate that protein farnesyltransferase transfers farnesyl residues to cysteine residues in tetrapeptides that conform to the sequence Cys-A l -A 2 -X, where A, and A 2 are aliphatic amino acids and X is methionine or serine. When the A 2 residue is aromatic (e.g. phenylalanine as in Cys-Val-Phe-Met) , the tetrapeptide continues to bind to the enzyme, but it can no longer accept a farnesyl group, and it becomes a pure inhibitor. The studies of the present example demonstrate that this resistance to farnesylation also requires a positive charge on the cysteine NH 2 -group. Derivatization of this group with acetyl, octanoyl, or cholic acid residues, or extension of the peptide with an additional amino acid, restores the ability of phenylalanine-containing peptides to accept a farnesyl residue. The same result was obtained when the NH 2 -group of cysteine was deleted (mercaptopropionic acid-Val-Phe- Met) . These data suggest that the positive change on the cysteine amino group acts in concert with an aromatic residue in the A 2 position to render peptides resistant to farnesylation. Therefore, it can be concluded from these studies that a nonfarnesylated tetrapeptide inhibitor of this type must contain both an aromatic residue at the A 2 position and a free NH 2 -terminus . 1. Methods a. Peptides Peptides were prepared by manual solid phase methodology (Barany, & Merrifield, 1980) using either t- butyloxycarbonyl (Boc) or 9-fluorenylmethyloxycarbonyl (Fmoc) chemistries, purified by reverse-phase high performance liquid chromatography (Amicon C18, 0.1% TFA/H 2 0/MeCN or 10 mM TEAA/H 2 0/MeCN) , and analyzed by fast atom bombardment mass spectrometry. Cholic acid (Aldrich) was activated using benzotriazol-l-yl-oxy-tris- (dimethylamino)phosphonium hexafluorophosphate (BOP) in N, N-dimethylacetamide. Octanoyl chloride was used to prepare the octanoyl peptide. Immediately before use, each peptide was dissolved at a concentration of 1 mM in dimethyl sulfoxide/10 mM dithiothreitol. All dilutions were made in water containing 10 mM dithiothreitol. b. Protein Farnesyltransferase Protein farnesyltransferase was purified to apparent homogeneity from rat brain homogenates by sequential ammonium sulfate fractionation, Mono Q ion-exchange chromatography, and peptide affinity chromatography as previously described (Reiss et al . , 1990a, 1990b) . c. Transfer of t^]Farnesyl from [^3 Farnesyl Pyrophosphate to Peptides Each 25-μl reaction mixture contained the following concentrations of components: 50 mM Tris-chloride (pH 7.5) , 50 μM ZnCl 2 , 3 mM MgCl 2 , 20 mM KCl, 1 mM dithiothreitol, 0.2% (v/v) octyl β-D-glucoside, either 0.6 or 2.4 μM all- trans- [ 3 H] farnesyl pyrophosphate (8,000- 16,000 dpm/pmol, Dupont-New England Nuclear) , the indicated concentration of peptide, and - 5 ng of affinity-purified protein farnesyltransferase. After incubation at 37°C for 15 or 30 min, the reaction was stopped by addition of 2 μl of 250 mM EDTA, and the entire reaction mixture was subjected to thin layer chromatography as previously described (Nagasawa et al . , 1984) . The origin (2-cm strip) and 12 sequential 1-cm fractions of each thin layer sheet were cut out and subjected to scintillation counting in 10 ml of 3a70B scintillation mixture (Research Products International) . The amount of [ 3 H] farnesyl attached to peptide was calculated by summing the radioactivity in the peak fractions (typically fractions 10 to 12 or 9 to 11, depending on the peptide) . Blank values were determined in parallel incubations that contained either no peptide or a tetrapeptide (SVIM) that is not a substrate for farnesylation (Nagasawa et al . , 1984) . d. Assay for Protein Farnesyltransferase Activity The amount of [ 3 H] farnesyl transferred from all- trans- [ 3 H] farnesyl pyrophosphate to recombinant p21 H"ras was measured in a filter assay as previously described (Reiss et al . , 1990) . 2. Results As set forth in examples above, the attachment of [ 3 H] farnesyl to peptides is preferably measured by thin layer chromatography to determine which inhibitors were also good substrates for the enzyme. Figure 23 compares two peptides, CVFM and N-AcCVFM, in their ability to inhibit the transfer of [ 3 H] farnesyl to p21 H"ras produced in E. coli (Panel A) and their ability to act as acceptors for [ 3 H] farnesyl in a direct transfer assay ( Panel B) when incubated with purified protein farnesyltransferase isolated from rat brain. Incorporation of [ 3 H] farnesyl into p21 H"ras was measured following precipitation with trichloroacetic acid. Both peptides inhibited farnesylation of p21 H"ras with relatively high affinity. The concentrations giving 50% inhibition were 0.07 and 0.27 μM for CVFM and N-AcCVFM, respectively. In the thin layer chromatography assay used in Fig. 23B, [ 3 H] farnesylated peptides migrate near the solvent front, and unincorporated [ 3 H] FPP remains near the origin (Goldstein et al . , 1991) . In the presence of CVIM nearly all of the 3 H-radioactivity migrated with the peptide. As before, aromatic substitution at A 2 (i.e., CVFM) blocked farnesyl transfer. Acetylation of the NH 2 - terminus (i.e., N-AcCVFM) restored farnesylation of this potent inhibitor (Fig. 23B) . Figure 24 shows that the same discrepancy in inhibitory activity and acceptor function was also seen for CIFM and N-AcCIFM, which differ from the peptides in Fig. 23 by the substitution of isoleucine for valine at the At position. Again, the two peptides inhibited farnesylation of p21 H " ras , but only the acetylated peptide (which was the less potent inhibitor of farnesylation) was farnesylated. It is interesting that two spatially separate domains of the peptide, the NH 2 -terminus and A 2 aromatic side chain, together block farnesyl transfer, while either alone yields fully functional substrate. Also, the data in Table IV (see below) show little correlation between inhibitor potency and substrate activity, indicating that peptide binding and farnesyltransferase activity are defined by distinct peptide-protein interactions. Table IV compares the farnesyl acceptor activity and the farnesyltransferase inhibitory activity of a series of N-modified peptides at a high peptide concentration (3.6 μM) . The assays were performed on several occasions with different preparations of purified farnesyltransferase. In order to standardize the results, in each study the farnesylation of a standard peptide CVIM (which corresponds to the COOH-terminus of p21 κ"rasB ) was measured and the results expressed as a ratio of [ 3 H] farnesyl incorporated into the test peptide vs . the standard peptide. The data show that the ability of N- acetylation to restore farnesylation was not restricted to peptides containing phenylalanine. The same type of result was obtained with tryptophan (CVWM and N-AcCVWM) . Moreover, the N-substituent was not restricted to the acetyl group. A similar result was obtained when the substituent on CVFM was an octanoyl or a cholic acid residue. Farnesylation was also enabled when the N- substituent was an amino acid, creating a pentapeptide such as KCVFM or CCVFM. The N-acetylated derivatives of these and other pentapeptides that contained CVFM were also farnesylated. TABLE IV INTERACTION OF N-MODIFIED PEPTIDES WITH PROTEIN FARNESYLTRANSFERASE Peptide Ratio of Concentration for [ 3 H] Farnesyl 50% Inhibition of Incorporated Into Farnesylation of Peptide/CVIM p2 fl (μM) CVIM 1.0 N-AccVIM 1.8 CVWM 0 N-AccCVWM 1.2 CVFM 0 N-CholylCVFM 0.85 N-AcCVFM 1.1 N-Octanoyl-CVFM 2.1 N-AcHCVFM 0.96 N-AcCCVFM 1.2 N-AcDCVFM 1.3 N-AcECVFM 1.4 N-AcPCVFM 1.4 N-AcGCVFM 1.9 N-AcSCVFM 2.0 N-AcMCVFM 2.2 N-AcKCVFM 2.8 Peptide Ratio of Concentration for [ 3 H] Farnesyl 50% Inhibition of Incorporated Into Farnesylation of Peptide/CVIM p2ι"ϊ (μM) KCVFM 0.77 1.1 CCVFM 1.5 0.44 Each peptide was incubated at a concentration of 3.6 μM with purified farnesyltransferase, and the incorporated radioactivity was determined as described above. For purposes of standardization, the data are expressed as the ratio of incorporation of [ 3 H] farnesyl from [ 3 H] farnesyl pyrophosphate into each tetrapeptides divided by the incorporation into CVIM, which was measured in each experiment. The values for 50% inhibition of the farnesylation of p21 H"ras were obtained from experiments in which each peptide was tested at six concentrations ranging from 0.03 to 10 μM. Mean of 9 consecutive experiments over a 3- month period in which the 50% inhibition values ranged from 0.032 to 0.09 μM. Figure 25 shows that a phenylalanine-containing tetrapeptide with a free NH 2 -group (CVFM) inhibited the farnesylation of an N-substituted peptide (N- OctanoylCVFM) , further confirming the effect of N- modification on the farnesylation reaction. The result also suggests that CVFM and its N-substituted derivative interact with the same binding site on the enzyme. Discussion The foregoing examples demonstrate that peptides that contain aromatic residues in the A 2 position of the QA X A 2 X sequence inhibit farnesylation of p21 H " ras without themselves becoming farnesylated by the enzyme. In the present example it is shown that the resistance of these peptides to farnesylation also depends upon the presence of a free NH 2 -terminus on cysteine. Substitution of this NH 2 -group with acyl (acetyl or octanoyl) or amino acid residues allows the peptide to become a substrate for farnesylation. The farnesyltransferase enzyme displays two rather remarkable specificities. First, the wide variation in inhibitory activity over the range of sequences tested denotes a precise peptide recognition domain. Second, the A 2 aromatic group and the NH 2 -terminus must act in concert to disrupt farnesyl transfer. Separately, peptides with either of these moieties are well tolerated as substrates. Further modification at these two sites of the peptide may allow one to separately probe the structural requirements for enzyme binding and farnesyl transfer. It is likely that the resistance of the aromatic- containing peptides to farnesylation requires a positive charge at the NH 2 -terminus. All of the modifications that restore farnesylation also remove the positive charge on this nitrogen. This interpretation is also consistent with a previous result in which a phenylalanine- containing peptide that lacks a primary NH 2 -group (mercaptopropionic acid-VFM) was farnesylated. The mechanism whereby the positively-charged phenylalanine-containing peptides resist farnesylation is not known. One possibility is that the binding of a peptide with aromatic residues in the A 2 position causes a conformational change in the enzyme that places the charged NH 2 -terminus in position to disrupt transfer of the farnesyl residue. Alternatively, it is possible that the peptides are farnesylated, but that they dissociate slowly from the enzyme, thereby preventing repeated cycles of farnesylation. These possibilities should be distinguishable by careful kinetic studies with the purified farnesyltransferase. In a practical sense the current findings raise questions regarding the use of aromatic-containing peptides such as CVFM to inhibit farnesyltransferase in intact cells. Non-substrate peptide inhibitors may be preferable for delivery into cells, and the requirement for a charged NH 2 -terminus may retard passage of the peptide through the cell membrane. It may be necessary to mask this charge with a substituent that will be cleaved within the cell. Considering the ubiquity of esterases and amidases, this goal should be attainable. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the composition, methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents which are both chemically and physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims. REFERENCES The references listed below are incorporated herein by reference to the extent that they supplement, explain, provide a background for or teach methodology, techniques and/or compositions employed herein. Akada, R. , et al . (1989), Mol. Cell. Biol.. 1:3491-3498. Akusjarvi, G. , Svensson, C . , and Nygard, O. (1987) , Mol . Cell. Biol. 7, 549-551. Andersson, S., Davis, D. L., Dahlback, H., Jδrnvall, H. & Russell, D. W. (1989), J. Biol. Chem. 264, 8222-8229. Ashby, M.N., and Edwards, P.A. (1990), J. Biol. Chem. 265. 13157-13164. Aviv, H., et al . (1972) , Proc. Natl. Acad. 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Stewart, J.M. et al . (1984) , Solid Phase Peptide Synthesis, 2nd ed. , Pierce Chemical Co., Rockford, IL. Tabor, S., et al. (1987), Proc. Natl. Acad. Sci. USA, 84:4767-4771. Ya ane, H.K., et al . (1990) , Proc. Natl. Acad. Sci. USA, 87:5868-5872. SEOUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANTS: (A) NAME: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS (B) STREET: 201 West 7th Street (C) CITY: Austin (D) STATE: TEXAS (E) COUNTRY: UNITED STATES OF AMERICA (F) POSTAL CODE: 78701 and (A) NAME: GENENTECH, INC. (B) STREET: 460 Point San Bruno Blvd. (C) CITY: San Franciso (D) STATE: California (E) COUNTRY: UNITED STATES OF AMERICA (F) ZIP: 94080 (ii) INVENTORS BROWN, MICHAEL S. GOLDSTEIN, JOSEPH L. REISS, YUVAL MARSTERS, JR., JAMES C. (iii) TITLE OF INVENTION: METHODS AND COMPOSITIONS FOR THE IDENTIFICATION, CHARACTERIZATION AND INHIBITION OF FARNESYLTRANSFERASE (iv) NUMBER OF SEQUENCES: 71 (V) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: ARNOLD, WHITE & DURKEE (B) STREET: P.O. BOX 4433 (C) CITY: HOUSTON (D) STATE: TEXAS (E) COUNTRY: UNITED STATES OF AMERICA (F) ZIP: 77210 (vi) COMPUTER READABLE FORM: (A) MEDIUM TYPE: FLOPPY DISK/ASKII (B) COMPUTER: IBM PC COMPATIBLE (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: WORDPERFECT 5.1 (vii) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: UNKNOWN (B) FILING DATE: AUGUST 24, 1993 (C) CLASSIFICATION: UNKNOWN (vϋi) PRIOR APPLICATION DATA: (A) APPLICATION NUMBER: 07/935,087 (B) FILING DATE: 24 AUGUST 1992 (24.08.92) (C) CLASSIFICATION: UNKNOWN (ix) ATTORNEY/AGENT INFORMATION: (A) NAME: PARKER, DAVID L. (B) REGISTRATION NUMBER: 32,165 (C) REFERENCE/DOCKET NUMBER: UTFD377PCT (x) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 512-320-7200 (B) TELEFAX: 512-474-7577 (C) TELEX: NOT APPLICABLE (2) INFORMATION FOR SEQ ID NO: 1 : (i) SEQUENCE CHARACTERISTICS: 5 (A) LENGTH: 377 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear 10 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: Met Ala Ala Thr, Glu Gly Val Gly Glu Ser Ala Pro Gly Gly Glu Pro 1 ' 5 10 15 15 Gly Gin Pro Glu Gin Pro Pro Pro Pro Pro Pro Pro Pro Pro Ala Gin 20 25 30 c I Gin Pro Gin Glu Glu Glu Met Ala Ala Glu Ala Gly Glu Ala Ala Ala 20 35 40 45 Ser Pro Met Asp Asp Gly Phe Leu Ser Leu Asp Ser Pro Thr Tyr Val 50 55 60 25 Leu Tyr Arg Asp Arg Ala Glu Trp Ala Asp lie Asp Pro Val Pro Gin 65 70 75 80 Asn Asp Gly Pro Ser Pro Val Val Gin lie lie Tyr Ser Glu Lys Phe 85 90 95 30 Arg Asp Val Tyr Asp Tyr Phe Arg Ala Val Leu Gin Arg Asp Glu Arg 100 105 110 Ser Glu Arg Ala Phe Lys Leu Thr Arg Asp Ala lie Glu Leu Asn Ala 35 115 120 125 10 15 I H H -J I 20 25 30 35 Asp Met Leu Glu Asn Gin Cys Asp Asn Lys Glu Asp He Leu Asn Lys 325 330 335 Ala Leu Glu Leu Cys Glu He Leu Ala Lys Glu Lys Asp Thr He Arg 5 340 345 350 Lys Glu Tyr Trp Arg Tyr He Gly Arg Ser Leu Gin Ser Lys His Ser 355 360 365 10 Arg Glu Ser Asp He Pro Ala Ser Val 370 375 (3) INFORMATION FOR SEQ ID NO:2 : 15 (i) SEQUENCE CHARACTERISTICS: oo I 20 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: 25 GCGGGCCGCG GAGGGGGCGG GGCTCCACCA CCACCTCAGC TGCGGACGGA GGCGAGATGG 60 CGGCCACTGA GGGGGTCGGG GAATCTGCGC CAGGCGGTGA GCCGGGACAG CCAGAGCAGC 120 30 CGCCGCCCCC GCCTCCTCCG CCGCCAGCAC AGCAGCCGCA GGAAGAAGAG ATGGCGGCCG 180 AGGCCGGGGA AGCAGCGGCG TCCCCTATGG ACGACGGGTT TCTGAGCCTG GACTCGCCCA 240 CCTATGTCTT GTACAGGGAC AGGGCAGAGT GGGCTGACAT AGACCCAGTG CCCCAGAATG 300 35 ATGGCCCCAG TCCAGTGGTC CAGATCATCT ACAGTGAAAA GTTTAGAGAC GTCTATGATT 360 ACTTCCGAGC TGTTCTGCAG CGCGATGAAA GAAGCGAACG AGCCTTTAAG CTCACTCGAG 420 ATGCTATTGA GTTAAACGCA GCCAACTATA CGGTGTGGCA TTTTCGGAGA GTTCTCTTGA 480 GGTCGCTTCA GAAGGATCTG CAAGAAGAAA TGAACTACAT CATCGCAATA ATTGAGGAAC 540 AGCCCAAAAA CTATCAAGTT TGGCACCATA GGAGAGTATT AGTGGAGTGG CTGAAAGATC 600 10 CTTCTCAAGA GCTCGAGTTC ATCGCCGATA TCCTTAATCA GGATGCAAAG AATTACCATG 660 CCTGGCAGCA TCGACAGTGG GTCATTCAGG AGTTTCGACT TTGGGATAAT GAGCTGCAGT 720 15 ATGTGGACCA GCTTCTCAAA GAGGATGTGA GAAATAACTC TGTGTGGAAC CAAAGACACT 780 , Y- TCGTCATTTC TAATACCACT GGCTACAGTG ATCGCGCTGT GTTGGAGAGA GAAGTCCAAT 840 vo ATACTCTGGA AATGATCAAA TTAGTGCCAC ACAATGAGAG TGCGTGGAAC TACTTGAAAG 900 20 GGATTTTGCA GGACCGTGGT CTTTCCAGAT ACCCTAATCT ATTAAACCAG TTGCTTGATT 960 TACAACCAAG TCACAGCTCC CCCTACCTAA TTGCCTTTCT TGTGGATATC TATGAAGACA 1020 25 TGCTGGAAAA CCAGTGTGAC AACAAGGAGG ACATTCTTAA TAAAGCACTA GAGTTATGTG 1080 AGATTCTAGC TAAAGAAAAG GACACTATAA GAAAGGAATA TTGGAGATAT ATTGGACGGT 1120 CCCTCCAGAG TAAACACAGC AGAGAAAGTG ACATACCGGC GAGTGTATAG CAGCAAGAGC 1180 30 GGCTGGAAGA AGTGGACAAT GCTTTCTAAG GCCTCTTATT CGGGAGTGTA GAGCGGTTAG 1240 AGCGGTCATC TCATGCCTGT GAGCTAACGT TGTCCAGGTG CTGTTTCTAA CAAGAACTAA 1300 35 GGATGACTCC TGTGTCTGAC GCTGTTCAGA CTAGCTAAGA GTCGATTTCC TAAAGCAAGG 1360 TCATTGGAGG GGAGGGTGAA GAAAACTTTC CCGTAAAGGA ACTACTGCTT TTGTAGTCTT 1420 CCCAACATTT AATCCAATCC TGTAGAATCA GCATCTCCTG AAAGACATGG TGCACGGCTG 1480 TGTGCTGGGC GTGGCTAAGG GCAGCTGTGT CATGGGTTTG CAGTCATGGG AACCTCGGAG 1560 CGCTGCCCGG GACTGCATTG ATGATTAGGG CTGCTGGCCT CACCCACAGG ATCTTGCTAT 1620 CACTGTAACC AACTAATGCC AAAAGAAAGG TTTTATAATA AAATCACATT ATCTACAAAC 1680 10 AAAAAAAAAA AAAAAAAAAA A 1701 (4) INFORMATION FOR SEQ ID NO:3: 15 (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 437 amino acid residues (B) TYPE: amino acid 20 (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3 25 Met Ala Ser Ser Ser Ser Phe Thr Tyr Tyr Cys Pro Pro Ser Ser Ser 1 5 10 15 Pro Val Trp Ser Glu Pro Leu Tyr Ser Leu Arg Pro Glu His Ala Arg 30 20 25 ' 30 Glu Arg Leu Gin Asp Asp Ser Val Glu Thr Val Thr Ser He Glu Gin 35 40 45 35 Ala Lys Val Glu Glu Lys He Gin Glu Val Phe Ser Ser Tyr Lys Phe 50 55 60 Asn His Leu Val Pro Arg Leu Val Leu Gin Arg Glu Lys His Phe His 65 70 75 80 5 Tyr Leu Lys Arg Gly Leu Arg Gin Leu Thr Asp Ala Tyr Glu Cys Leu 85 90 95 Asp Ala Ser Arg Pro Trp Leu Cys Tyr Trp He Leu His Ser Leu Glu 10 100 105 110 Leu Leu Asp Glu Pro He Pro Gin He Val Ala Thr Asp Val Cys Gin 115 120 125 15 Phe Leu Glu Leu Cys Gin Ser Pro Asp Gly Gly Phe Gly Gly Gly Pro , 130 135 140 H Gly Gin Tyr Pro His Leu Ala Pro Thr Tyr Ala Ala Val Asn Ala Leu I 145 150 155 160 20 Cys He He Gly Thr Glu Glu Ala Tyr Asn Val He Asn Arg Glu Lys 165 170 175 Leu Leu Gin Tyr Leu Tyr Ser Leu Lys Gin Pro Asp Gly Ser Phe Leu 25 180 185 190 Met His Val Gly Gly Glu Val Asp Val Arg Ser Ala Tyr Cys Ala Ala 195 200 205 30 Ser Val Ala Ser Leu Thr Asn He He Thr Pro Asp Leu Phe Glu Gly 210 215 220 Thr Ala Glu Trp He Ala Arg Cys Gin Asn Trp Glu Gly Gly He Gly 225 230 235 240 35 Gly Val Pro Gly Met Glu Ala His Gly Gly Tyr Thr Phe Cys Gly Leu 245 250 255 Ala Ala Leu Val He Leu Lys Lys Glu Arg Ser Leu Asn Leu Lys Ser 5 260 265 270 Leu Leu Gin Trp Val Thr Ser Arg Gin Met Arg Phe Glu Gly Gly Phe 275 280 285 10 Gin Gly Arg Cys Asn Lys Leu Val Asp Gly Cys Tyr Ser Phe Trp Gin 290 295 300 Ala Gly Leu Leu Pro Leu Leu His Arg Ala Leu His Ala Gin Gly Asp 305 310 315 320 15 Pro Ala Leu Ser Met Ser His Trp Met Phe His Gin Gin Ala Leu Gin 325 330 335 to Glu Tyr He Leu Met Cys Cys Gin Cys Pro Ala Gly Gly Leu Leu Asp 20 340 345 350 Lys Pro Gly Lys Ser Arg Asp Phe Tyr His Thr Cys Tyr Cys Leu Ser 355 360 365 25 Gly Leu Ser He Ala Gin His Phe Gly Ser Gly Ala Met Leu His Asp 370 375 380 Val Val Met Gly Val Pro Glu Asn Val Leu Gin Pro Thr His Pro Val 385 390 395 400 30 Tyr Asn He Gly Pro Asp Lys Val He Gin Ala Thr Thr His Phe Leu 405 410 415 Gin Lys Pro Val Pro Gly Phe Glu Glu Cys Glu Asp Ala Val Thr Ser Asp Pro Ala Thr Asp 435 5 (5) INFORMATION FOR SEQ ID NO:4 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2464 base pairs 10 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4; 15 I H CGGGCGCGTT GTTGCTGGAC GAAGCTGAGT CCTATACAGC GCTCGCAGCT CTCCCGATCA 60 ω TGGCTTCTTC GAGTTCCTTC ACCTATTATT GTCCTCCATC TTCTTCCCCT GTTTGGTCAG 120 20 AACCGCTGTA TAGTCTGAGA CCTGAGCACG CGCGGGAGCG GTTGCAAGAC GACTCAGTGG 180 AAACAGTCAC GTCCATAGAA CAGGCCAAAG TAGAAGAAAA GATCCAGGAG GTCTTCAGTT 240 25 CTTACAAGTT TAACCACCTC GTACCAAGGC TCGTTCTGCA GAGGGAGAAG CACTTCCATT 300 ATCTGAAAAG AGGCCTTCGA CAACTGACAG ATGCCTATGA GTGTCTGGAT GCCAGCCGCC 360 CCTGGCTCTG CTACTGGATC CTGCACAGCT TGGAGCTCCT CGACGAACCC ATCCCCCAAA 420 30 TAGTGGCTAC AGATGTGTGT CAGTTCTTGG AGCTGTGTCA GAGTCCAGAC GGTGGCTTTG 480 GAGGGGGCCC TGGTCAGTAC CCACACCTCG CTCCCACGTA TGCAGCTGTC AACGCGCTAT 540 35 GCATCATTGG CACGGAGGAA GCCTACAACG TCATTAACAG AGAGAAGCTC CTTCAGTACT 600 TGTACTCCCT AAAGCAACCG GATGGCTCTT TTCTCATGCA CGTCGGAGGA GAGGTGGATG 660 TAAGAAGTGC GTACTGTGCT GCCTCAGTAG CCTCTCTCAC CAACATCATC ACTCCTGACC 720 TCTTCGAAGG CACTGCTGAA TGGATAGCAA GGTGCCAGAA CTGGGAAGGC GGCATTGGCG 780 GGGTGCCAGG GATGGAAGCC CACGGTGGCT ACACCTTCTG TGGCTTGGCT GCGCTGGTGA 840 TCCTCAAGAA GGAACGTTCT TTGAACCTGA AGAGCTTGCT ACAATGGGTG ACAAGCCGGC 900 10 AGATGCGGTT CGAAGGAGGA TTTCAGGGCC GCTGCAACAA GCTGGTGGAC GGCTGCTACT 960 CCTTCTGGCA GGCAGGACTT CTGCCCCTGT TGCACCGGGC ACTCCACGCT CAAGGTGACC 1020 15 CTGCCCTCAG CATGAGCCAC TGGATGTTCC ATCAGCAGGC GCTGCAGGAG TACATCCTCA 1080 , TGTGCTGCCA GTGTCCGGCT GGGGGTCTCC TGGACAAACC TGGCAAGTCA CGTGACTTCT 1140 I ACCATACTTG CTACTGCCTG AGCGGCCTGT CCATTGCCCA GCATTTTGGA AGTGGAGCCA 1200 20 TGCTGCACGA TGTGGTCATG GGTGTGCCTG AAAATGTTCT GCAGCCCACT CACCCTGTGT 1260 ACAACATCGG ACCTGATAAG GTGATCCAGG CCACCACACA CTTTCTGCAG AAGCCGGTCC 1320 25 CAGGCTTTGA GGAATGCGAA GATGCGGTGA CCTCAGATCC TGCCACTGAC TAGAGGACCC 1380 CATGGCTCCC CCAAATCCCC CGTCAGACAA GGTTTCTCCG TTTGGGTACA TAGCACAGTC 1440 CGTGCTACTT GAGCCTTGGC CACTGTGGAG TTGTGGTTTC TTTGTCCTTT CCTGTCAAAC 1500 30 AAAACAAAGC CATCAGCTCT GGGTTGGAAT ACACAATGGT GTGATTTTTA AAATTATTTT 1560 CATACCTGTC AAACCAAAAC TCTGGGAGCC GATGTAGTAA GCAGGGTTGG AGAGCAATGC 1620 35 ATGCTGGGAA GCAGCAGCCT CCTCCAGCAG CCAGGCCCAC AATGCTGAAA TGGAAGGTGT 1680 CTGTGAGTAT CTCCACATCA CAGCCACTGC TGTGCCTCCC ACCTACACAC CATTCAGTCA 1740 GCAGATGGGC TCCTCTCTGG TATAAATGTC AGCTCTGTGC AAGGGCGGCG CTGTGGGTCC 1800 AGCCAATACA CGCTCTCTGG AAAACAGCAC TGGGCTCCAG TGGGCATATT CATACTTGTC 1860 TCTTTTACCC CAGTCATTTG CGAAGGACAG GGGCCAGGAA TGAAGAAGGG TCTTAGATTG 1920 AGCCCTCCCC ACAACCTGAG GAGAACTGAT CTCATATTTC TCCAAGGCCA TGTTTGTATG 1980 10 AGCAAGACTT GTTTTGCCCT AAGTATGACG ACTAGACCCA GGTAATCAAT TATGAGTGGA 2040 AAATCAACCT CTAGGTGAAC TCTGTGCCAG AGGAAGCAGC CTCCCCAGTG TCCAGCCCCC 2100 15 GCCTCTCCCC ATCATGTACC AGGAGAGGCC CTCCTCACGG CAGTGCTGCA GCCCAGGCTC 2160 , CTTCTAGTCC TTTCTCCCCA CCCACCCTCC AAGACAGTGC TCTTTTCTCA TCCAGGGTGT 2220 n I TAACACTACT AAGGCTTCAC CGTAATCGAT CACTCAGGAT TTACTCCTGC CCTGCCCACT 2280 20 CCAGGCTCTC TGAAACACAG TCAAGTGCTA GGCAAGCTAG CTGCTGCTGG GACAGTGACC 2340 AGCAGGAAGG CAAGTGCTGT CCGTGTGCTG AATTCTGGAA CTGCCTCTGC ACCGGCTGAG 2400 25 TTTGCTCACC TATCCACTGC TACAGTCATA GCAAGCTCAT GCCGCTGTCC CAGCCTGTGC 2460 GAGC 2464 30 (6) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 379 amino acid residues 35 (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: 5 Met Ala Ala Thr Glu Gly Val Gly Glu Ala Ala Gin Gly Gly Glu Pro 1 5 10 15 10 Gly Gin Pro Ala Gin Pro Pro Pro Gin Pro His Pro Pro Pro Pro Gin 20 25 30 Gin Gin His Lys Glu Glu Met Ala Ala Glu Ala Gly Glu Ala Val Ala 35 40 45 15 Ser Pro Met Asp Asp Gly Phe Val Ser Leu Asp Ser Pro Ser Tyr Val 50 55 60 σ Leu Tyr Arg Asp Arg Ala Glu Trp Ala Asp He Asp Pro Val Pro Gin 20 65 70 75 80 Asn Asp Gly Pro Asn Pro Val Val Gin He He Tyr Ser Asp Lys Phe 85 90 95 25 Arg Asp Val Tyr Asp Tyr Phe Arg Ala Val Leu Gin Arg Asp Glu Arg 100 105 110 Ser Glu Arg Ala Phe Lys Leu Thr Arg Asp Ala He Glu Leu Asn Ala 115 120 125 30 Ala Asn Tyr Thr Val Trp His Phe Arg Arg Val Leu Leu Lys Ser Leu 130 135 140 Gin Lys Asp Leu His Glu Glu Met Asn Tyr He Thr Ala He He Glu 35 145 150 155 160 Glu Gin Pro Lys Asn Tyr Gin Val Trp His His Arg Arg Val Leu Val Glu Trp Leu Arg 180 Leu Asn Gin Asp Ala Lys Asn Tyr His Ala Trp Gin His Arg Gin Trp 195 200 205 10 Val He Gin Glu Phe Lys Leu Trp Asp Asn Glu Leu Gin Tyr Val Asp 210 215 220 Gin Leu Leu Lys Glu Asp Val Arg Asn Asn Ser Val Trp Asn Gin Arg 225 230 235 240 15 I Tyr Phe Val He Ser Asn Thr Thr Gly Tyr Asn Asp Arg Ala Val Leu t 245 250 255 I Glu Arg Glu Val Gin Tyr Thr Leu Glu Met He Lys Leu Val Pro His 20 260 265 270 Asn Glu Ser Ala Trp Asn Tyr Leu Lys Gly He Leu Gin Asp Arg Gly 275 280 285 25 Leu Ser Lys Tyr Pro Asn Leu Leu Asn Gin Leu Leu Asp Leu Gin Pro 290 295 300 Ser His Ser Ser Pro Tyr Leu He Ala Phe Leu Val Asp He Tyr Glu 305 310 315 320 30 Asp Met Leu Glu Asn Gin Cys Asp Asn Lys Glu Asp He Leu Asn Lys 325 330 335 Ala Leu Glu Leu Cys Glu He Leu Ala Lys Glu Lys Asp Thr He Arg Lys Glu Tyr Trp Arg Tyr He Gly Arg Ser Leu Gin Ser Lys His Ser 355 360 365 Thr Glu Asn Asp Ser Pro Thr Asn Val Gin Gin 5 370 375 (7) INFORMATION FOR SEQ ID NO:6 : 10 (i) SEQUENCE CHARACTERISTICS: lj I (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: 20 ATGGCGGCCA CCGAGGGGGT CGGGGAGGCT GCGCAAGGGG GCGAGCCCGG GCAGCCGGCG 60 CAACCCCCGC CCCAGCCGCA CCCACCGCCG CCCCAGCAGC AGCACAAGGA AGAGATGGCG 120 GCCGAGGCTG GGGAAGCCGT GGCGTCCCCC ATGGACGACG GGTTTGTGAG CCTGGACTCG 180 25 CCCTCCTATG TCCTGTACAG GGACAGAGCA GAATGGGCTG ATATAGATCC GGTGCCGCAG 240 AATGATGGCC CCAATCCCGT GGTCCAGATC ATTTATAGTG ACAAATTTAG AGATGTTTAT 300 30 GATTACTTCC GAGCTGTCCT GCAGCGTGAT GAAAGAAGTG AACGAGCTTT TAAGCTAACC 360 CGGGATGCTA TTGAGTTAAA TGCAGCCAAT TATACAGTGT GGCATTTCCG GAGAGTTCTT 420 TTGAAGTCAC TTCAGAAGGA TCTACATGAG GAAATGAACT ACATCACTGC AATAATTGAG 480 35 GAGCAGCCCA AAAACTATCA AGTTTGGCAT CATAGGCGAG TATTAGTGGA ATGGCTAAGA 540 GATCCATCTC AGGAGCTTGA ATTTATTGCT GATATTCTTA ATCAGGATGC AAAGAATTAT 600 CATGCCTGGC AGCATCGACA ATGGGTTATT CAGGAATTTA AACTTTGGGA TAATGAGCTG 660 CAGTATGTGG ACCAACTTCT GAAAGAGGAT GTGAGAAATA ACTCTGTCTG GAACCAAAGA 720 TACTTCGTTA TTTCTAACAC CACTGGCTAC AATGATCGTG CTGTATTGGA GAGAGAAGTC 780 10 CAATACACTC TGGAAATGAT TAAACTAGTA CCACATAATG AAAGTGCATG GAACTATTTG 840 AAAGGGATTT TGCAGGATCG TGGTCTTTCC AAATATCCTA ATCTGTTAAA TCAATTACTT 900 15 GATTTACAAC CAAGTCATAG TTCCCCCTAC CTAATTGCCT TTCTTGTGGA TATCTATGAA 960 , GACATGCTAG AAAATCAGTG TGACAATAAG GAAGACATTC TTAATAAAGC ATTAGAGTTA 1020 ω TGTGAAATCC TAGCTAAAGA AAAGGACACT ATAAGAAAGG AATATTGGAG ATACATTGGA 1080 20 AGATCCCTTC AAAGCAAACA CAGCACAGAA AATGACTCAC CAACAAATGT ACAGCAATAA 1140 CACCATCCAG AAGAACTTGA TGGAATGCTT TTATTTTTTA TTAAGGGACC CTGCAGGAGT 1200 25 TTCACACGAG AGTGGTCCTT CCCTTTGCCT GTGGTGTAAA AGTGCATCAC ACAGGTATTG 1260 CTTTTTAACA AGAACTGATG CTCCTTGGGT GCTGCTGCTA CTCAGACTAG CTCTAAGTAA 1320 TGTGATTCTT CTAAAGCAAA GTCATTGGAT GGGAGGAGGA AGAAAAAGTC CCATAAAGGA 1380 30 ACTTTTGTAG TCTTATCAAC ATATAATCTA ATCCCTTAGC ATCAGCTCCT CCCTCAGTGG 1440 TACATGCGTC AAGATTTGTA GCAGTAATAA CTGCAGGTCA CTTGTATGTA ATGGATGTGA 1500 35 GGTAGCCGAA GTTTGGTTCA GTAAGCAGGG AATACAGTCG TTCCATCAGA GCTGGTCTGC 1560 ACACTCACAT TATCTTGCTA TCACTGTAAC CAACTAATGC CAAAAGAACG GTTTTGTAAT 1620 AAAATTATAG CTGTATCTAA AAAAAAAAAA AAAAAAAAAA AAAA 1664 (8) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: 10 (A) LENGTH: 387 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear 15 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: £ o Val Glu Glu Lys He Gin Glu Val Phe Ser Ser Tyr Lys Phe Asn His ' 1 5 10 15 20 Leu Val Pro Arg Leu Val Leu Gin Arg Glu Lys His Phe His Tyr Leu 20 25 30 Lys Arg Gly Leu Arg Gin Leu Thr Asp Ala Tyr Glu Cys Leu Asp Ala 25 35 40 45 Ser Arg Pro Trp Leu Cys Tyr Trp He Leu His Ser Leu Glu Leu Leu 50 55 60 30 Asp Glu Pro He Pro Gin He Val Ala Thr Asp Val Cys Gin Phe Leu 65 70 75 80 Glu Leu Cys Gin Ser Pro Glu Gly Gly Phe Gly Gly Gly Pro Gly Gin 85 90 95 35 Tyr Pro His Leu Ala Pro Thr Tyr Ala Ala Val Asn Ala Leu Cys He 100 105 110 He Gly Thr Glu Glu Ala Tyr Asp He He Asn Arg Glu Lys Leu Leu 5 115 120 125 Gin Tyr Leu Tyr Ser Leu Lys Gin Pro Asp Gly Ser Phe Leu Met His 130 135 140 10 Val Gly Gly Glu Val Asp Val Arg Ser Ala Tyr Cys Ala Ala Ser Val 145 150 155 160 Ala Ser Leu Thr Asn He He Thr Pro Asp Leu Phe Glu Gly Thr Ala 165 170 175 15 Glu Trp He Ala Arg Cys Gin Asn Trp Glu Gly Gly He Gly Gly Val 180 185 190 H I Pro Gly Met Glu Ala His Gly Gly Tyr Thr Phe Cys Gly Leu Ala Ala 20 195 200 205 Leu Val He Leu Lys Arg Glu Arg Ser Leu Asn Leu Lys Ser Leu Leu 210 215 220 25 Gin Trp Val Thr Ser Arg Gin Met Leu Phe Glu Gly Gly Phe Gin Gly 225 230 235 240 Arg Cys Asn Lys Leu Val Asp Gly Cys Tyr Ser Phe Trp Gin Ala Gly 245 250 255 30 Leu Leu Pro Leu Leu His Arg Ala Leu His Ala Gin Gly Asp Pro Ala 260 265 270 Leu Ser Met Ser His Trp Met Phe His Gin Gin Ala Leu Gin Glu Tyr He Leu Met Cys Cys Gin Cys Pro Ala Gly Gly Leu Leu Asp Lys Pro 290 295 300 Gly Lys Ser Arg Asp Phe Tyr His Thr Cys Tyr Cys Leu Ser Gly Leu 5 305 310 315 320 Ser He Ala Gin His Phe Gly Ser Gly Ala Met Leu His Asp Val Val 325 330 335 10 Leu Gly Val Pro Glu Asn Ala Leu Gin Pro Thr His Pro Val Tyr Asn 340 345 350 He Gly Pro Asp Lys Val He Gin Ala Thr Thr Tyr Phe Leu Gin Lys 355 360 365 15 Pro Val Pro Gly Phe Glu Glu Leu Lys Asp Glu Thr Ser Ala Glu Pro 370 375 380 w Ala Thr Asp 20 385 (9) INFORMATION FOR SEQ ID NO: 8 : 25 (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1248 nucleic acids (B) TYPE: nucleic acid (C) STRANDEDNESS: single 30 (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: 35 GTAGAAGAAA AGATCCAAGA GGTCTTCAGT TCTTACAAGT TCAACCACCT TGTACCAAGG 60 CTTGTTTTGC AGAGGGAGAA GCACTTCCAT TATCTGAAAA GAGGCCTTCG ACAACTGACA 120 GATGCCTATG AGTGTCTGGA TGCCAGCCGC CCATGGCTCT GCTATTGGAT CCTGCACAGC 180 TTGGAACTGC TAGATGAACC CATCCCCCAG ATAGTGGCTA CAGATGTGTG TCAGTTCCTG 240 GAGCTGTGTC AGAGCCCAGA AGGTGGCTTT GGAGGAGGAC CCGGTCAGTA TCCACACCTT 300 GCACCCACAT ATGCAGCAGT CAATGCATTG TGCATCATTG GCACCGAGGA GGCCTATGAC 360 10 ATCATTAACA GAGAGAAGCT TCTTCAGTAT TTGTACTCCC TGAAGCAACC TGACGGCTCC 420 TTTCTCATGC ATGTCGGAGG TGAGGTGGAT GTGAGAAGCG CATACTGTGC TGCCTCCGTA 480 15 GCCTCGCTGA CCAACATCAT CACTCCAGAC CTCTTTGAGG GCACTGCTGA ATGGATAGCA 540 , AGGTGTCAGA ACTGGGAAGG TGGCATTGGC GGGGTACCAG GGATGGAAGC CCATGGTGGC 600 ω TATACCTTCT GTGGCCTGGC CGCGCTGGTA ATCCTCAAGA GGGAACGTTC CTTGAACTTG 660 20 AAGAGCTTAT TACAATGGGT GACAAGCCGG CAGATGCTAT TTGAAGGAGG ATTTCAGGGC 720 CGCTGCAACA AGCTGGTGGA TGGCTGCTAC TCCTTCTGGC AGGCGGGGCT CCTGCCCCTG 780 25 CTCCACCGCG CACTGCACGC CCAAGGTGAC CCTGCCCTTA GCATGAGCCA CTGGATGTTC 840 CATCAGCAGG CCCTGCAGGA GTACATCCTG ATGTGCTGCC AGTGCCCTGC GGGGGGGCTT 900 CTGGATAAAC CTGGCAAGTC GCGTGATTTC TACCACACCT GCTACTGCCT GAGCGGCCTG 960 30 TCCATAGCCC AGCACTTCGG CAGCGGAGCC ATGTTGCATG ATGTGGTCCT GGGTGTGCCC 1020 GAAAACGCTC TGCAGCCCAC TCACCCAGTG TACAACATTG GACCAGACAA GGTGATCCAG 1080 35 GCCACTACAT ACTTTCTACA GAAGCCAGTC CCAGGTTTTG AGGAGCTTAA GGATGAGACA 1140 TCGGCAGAGC CTGCAACCGA CTAGAGGACC TGGGTCCCGG CAGCTCTTTG CTCACCCATC 1200 TCCCCAGTCA GACAAGGTTT ATACGTTTCA ATACATACTG CATTCTGT 1248 5 I H ω I (10) INFORMATION FOR SEQ ID NO:9 : t (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: Thr Lys Cys Val He Met 1 5 (11) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino^ acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: Cys Val He Met 1 (12) INFORMATION FOR SEQ ID NO: 11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: Lys Lys Ser Lys Thr Lys Cys Val He Met 1 5 10 (13) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: Cys Xaa Xaa Xaa 1 (14) INFORMATION FOR SEQ ID NO:13: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13 Cys Ser He Met 1 (15) INFORMATION FOR SEQ ID NO:14 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14 Cys Ala He Met 1 (16) INFORMATION FOR SEQ ID NO: 15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15: Arg Ala Ser Asn Arg Ser Cys Ala He Met 1 5 10 (17) INFORMATION FOR SEQ ID NO:16 (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: Thr Gin Ser Pro Gin Asn Cys Ser He Met 1 5 10 ( 18 ) INFORMATION FOR SEQ ID NO : 17 : (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: Cys He He Met 1 (19) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18 Cys Val Val Met 1 (20) INFORMATION FOR SEQ ID NO: 19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19 Cys Val Leu Ser 1 (21) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20 Cys Val Leu Met 1 (22) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21: Cys Cys Val Gin 1 (23) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22 Cys He He Cys 1 (24) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23 Cys He He Ser 1 (25) INFORMATION FOR SEQ ID NO:24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24 Cys Val He Ser 1 (26) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25 Cys Val He Ala 1 (27) INFORMATION FOR SEQ ID NO:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26 Cys Val He Leu (28) INFORMATION FOR SEQ ID NO:27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27 Cys Leu He Leu 1 (29) INFORMATION FOR SEQ ID NO:28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28 Cys Leu Leu Leu 1 (30) INFORMATION FOR SEQ ID NO:29: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29 Cys Thr Val Ala 1 (31) INFORMATION FOR SEQ ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30 Cys Val Ala Met 1 (32) INFORMATION FOR SEQ ID NO:31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31 Cys Lys He Met 1 (33) INFORMATION FOR SEQ ID NO:32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32 Cys Leu He Met 1 (34) INFORMATION FOR SEQ ID NO:33: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33 Cys Phe He Met (35) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34 Cys Val Phe Met 1 (36) INFORMATION FOR SEQ ID NO:35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35 Cys Val He Phe 1 (37) INFORMATION FOR SEQ ID NO:36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36 Cys Glu He Met 1 (38) INFORMATION FOR SEQ ID NO:37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37 Cys Gly He Met 1 (39) INFORMATION FOR SEQ ID NO:38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38 Cys Pro He Met 1 (40) INFORMATION FOR SEQ ID NO:39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39 Cys Val Tyr Met 1 (41) INFORMATION FOR SEQ ID NO:40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40 Cys Val Thr Met (42) INFORMATION FOR SEQ ID NO:41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41 Cys Val Pro Met 1 (43) INFORMATION FOR SEQ ID NO:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42 Cys Val Ser Met 1 (44) INFORMATION FOR SEQ ID NO:43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43 Cys Val He Val 1 (45) INFORMATION FOR SEQ ID NO:44: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44 Cys Val He Pro 1 (46) INFORMATION FOR SEQ ID NO:45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO.-45: Cys Val He He 1 (47) INFORMATION FOR SEQ ID NO:46: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46 Cys Val Trp Met 1 (48) INFORMATION FOR SEQ ID NO:47: (i) SEQUENCE CHARACTERISTICS (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47 Cys He Phe Met (49) INFORMATION FOR SEQ ID NO:48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 12 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48: Cys Asn Phe Asp Asn Pro Val Ser Gin Lys Thr Thr 1 5 10 (50) INFORMATION FOR SEQ ID NO:49: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49 Thr Lys Val Cys He Met 1 5 (51) INFORMATION FOR SEQ ID NO:50: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 10 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 50: Lys Asn Asn Leu Lys Asp Cys Gly Leu Phe 1 5 10 (52) INFORMATION FOR SEQ ID NO:51: (i) SEQUENCE CHARACTERISTICS (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51 Cys Val Lys Met 1 (53) INFORMATION FOR SEQ ID NO:52: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52: Cys Val He Lys 1 (54) INFORMATION FOR SEQ ID NO:53: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53 Lys Thr Ser Cys Val He Met 1 5 (55) INFORMATION FOR SEQ ID NO:54: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54 Cys Xaa He Met 1 (56) INFORMATION FOR SEQ ID NO:55 (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 55 Cys Val Xaa Met 1 (57) INFORMATION FOR SEQ ID NO:56: (i) SEQUENCE CHARACTERISTICS : (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56 Cys Val He Xaa 1 (58) INFORMATION FOR SEQ ID NO:57: (i) SEQUENCE CHARACTERISTICS (A) LENGTH: 14 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57 GANGCNATNG ANNT 14 (59) INFORMATION FOR SEQ ID NO: 58 (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 14 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58: CNNAANTGCC ANAC 14 (60) INFORMATION FOR SEQ ID NO:59: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59 Asp Ala He Glu Leu Asn Ala Ala Asn Tyr Thr Val Trp His Phe Arg 1 5 10 15 (61) INFORMATION FOR SEQ ID NO:60: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 14 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:60 GCNTANTGNG CNGC 14 (62) INFORMATION FOR SEQ ID NO: 61: (i) SEQUENCE CHARACTERISTICS (A) LENGTH: 14 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:61: GGNGTNAGNA TNAT 14 (63) INFORMATION FOR SEQ ID NO:62: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 44 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 62 GCGTACTGTG CGGCCTCAGT AGCCTCTCTC ACCAACATNA TCAC 44 (64) INFORMATION FOR SEQ ID NO:63: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 16 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 63: Ala Tyr Cys Ala Ala Ser Val Ala Ser Leu Thr Asn He He Thr Pro 1 5 10 15 (65) INFORMATION FOR SEQ ID NO: 64: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 44 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 64 GANGCNATNG ANNTAAACGC ACGGAACTAT ACGGTCTGGC ACTT 44 (66) INFORMATION FOR SEQ ID NO: 65: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 65: Cys Ala He Leu 1 (67) INFORMATION FOR SEQ ID NO:66: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:66 Cys He Lys Ser 1 (68) INFORMATION FOR SEQ ID NO:67: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 15 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 67: Leu Gin Ser Lys His Ser Arg Glu Ser Asp He Pro Ala Ser Val 1 5 10 15 (69) INFORMATION FOR SEQ ID NO: 68: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:68: He Gin Ala Thr Thr His Phe Leu Gin Lys Pro Val Pro Gly Phe Glu 1 5 10 15 Glu (70) INFORMATION FOR SEQ ID NO: 69: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( i) SEQUENCE DESCRIPTION: SEQ ID NO:69 GACTCGAGTC GACATCGAT 19 (71) INFORMATION FOR SEQ ID NO: 70: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 7 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 70 Leu Xaa Asp Asp Xaa Xaa Glu 1 5 (72) INFORMATION FOR SEQ ID NO: 71: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 4 amino acid residues (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 71 Cys Xaa Xaa Leu 1

Claims: CLAIMS ; 1. A pure farnesyltransferase inhibitor comprising a compound having a farnesyltransferase inhibitor peptide sequence within its structure, said sequence being capable of inhibiting the farnesylation of p21^ by protein farnesyltransferase without itself serving as a substrate for' farnesylation by said enzyme, said farnesyltransferase inhibitor peptide sequence being defined as including the amino acids CA^X, wherein: C = cysteine; Al = any aliphatic, aromatic or hydroxy amino acid; A2 = any aromatic amino acid or amino acid modified to incorporate one or more aromatic moieties; and X = met, ser, glu or cys; wherein when said compound is introduced intracellularly into a target cell, the inhibitor is provided in a form wherein the C residue of CA J A J X is a positively charged amino terminus of the inhibitor. 2. The inhibitor of claim 1, wherein the compound comprises modified CAjA 2 X having an N-terminal modification that provides an uncharged alpha nitrogen on the cysteine residue, wherein said modification is removeable intracellularly by target cells to reveal an N-terminal cysteine having a positively charged alpha nitrogen. 3. The inhibitor of claim 2, further defined as capable of being modified by hydrolysis or deacylation to reveal an N-terminal cysteine having the positively charged alpha nitrogen. 4. The inhibitor of claim 3, wherein the inhibitor includes an N-mannich or Schiff base structure that is sensitive to hydrolysis. 5. The inhibitor of claim 4, further defined as having the following structure: R'CO--N--CH 2 --N--peptide H H wherein R' = phenyl, substituted phenyl, or alkyl. 6. The inhibitor of claim 4, further defined as having the following structure: ,N--peptide wherein R' = phenyl or alkyl 7. The inhibitor of claim 3, wherein the inhibitor includes a 2-substituted thiazolidine-4-carboxylic acid structure that is sensitive to hydrolysis. 8. The inhibitor of claim 7, wherein the inhibitor includes the following structure: wherein R H, alkyl, substituted alkyl, phenyl, substituted phenyl or pyridyl; and 9. The inhibitor of claim 3, further defined as comprising an N-terminal acyl group that is removeable by deacylation. 10. The inhibitor of claim 2, further defined as capable of being modified by mtracellular enzymatic action to reveal an N-terminal cysteine having the positively charged alpha nitrogen. 11. The inhibitor of claim 10, further defined as capable of being modified by an oxo-prolinase, esterase, acylase, aminopeptidase, trypsin, chymotrypsin, or transpeptidase, to reveal an N-terminal cystein having a positively charged alpha nitrogen. 12. The inhibitor of claim 11, further defined as capable of being modified by an oxo-prolinase, and having the structure: 13. The inhibitor of claim 11, further defined as capable of being modified by an esterase, and having the structure: R-N-C-A,-A 2 -X H wherein N = the alpha amino group of C; and 14. The inhibitor of claim 11, further defined as capable of being modified by a pyroglutamyl aminopeptidase, and having the structure: R-N-C-A,-A,-X H wherein N = the alpha amino group of C; and 15. The inhibitor of claim 11, further defined as capable of being modified by trypsin, and having the structure: R' -C-A J -A J -X wherein R' = L-arg, L-lys, or a peptide having a C-terminal L-arg or L-lys. 16. The inhibitor of claim 11, further defined as capable of being modified by chymotrypsin, and having the structure: R' -C-A ! -A 2 - wherein R' = L-phenylalanine, L-tyrosine, L- tryptophan, or a peptide having one of these amino acids at its C- terminal . 17. The inhibitor of claim 11, further defined as capable of being modified by a gamma-glutamyl transpeptidase, and having the structure: R-N-C-A ! -A 2 -X H wherein N = the alpha amino group of C; and 18. The inhibitor of claim 1, further defined as having the following structure: Rl-R2-R3-N-C-A A 2 -X wherein N = the alpha nitrogen of cysteine, and Rl, R2 or R3 = H, alkyl, substituted alkyl, phenyl, benzyl, substituted phenyl, or substituted benzyl. 19. The inhibitor of claim 1, further defined as having the following structure: wherein N = the alpha nitrogen of cysteine, 20. The inhibitor of claim 1, wherein the farnesyltransferase inhibitor peptide sequence is further defined as the tetrapeptide CVFM. 21. The inhibitor of claim 1, wherein the aromatic moiety of the A2 amino acid is modified to include a fluoro, chloro, or nitro group. 22. The inhibitor of claim 1, wherein the A2 amino acid comprises parachlorophenylalanine. 23. The inhibitor of claim 1, wherein the A2 amino acid includes a naphthyl ring. 24. A method of inhibiting a farnesyltransferase enzyme comprising subjecting the enzyme to an effective concentration of a farnesyltransferase inhibitor in accordance with claim 1. 25. A method of inhibiting the attachment of a farnesyl moiety to a p21ras protein in malignant cells comprising subjecting said cells to an effective concentration of a farnesyltransferase inhibitor in accordance with claim 1.

Inventors: BROWN MICHAEL S, GOLDSTEIN JOSEPH L, MARSTERS JAMES C JR, REISS YUVAL, BROWN, MICHAEL, S., GOLDSTEIN, JOSEPH, L., MARSTERS, JAMES, C., JR., REISS, YUVAL
Applicants: BROWN MICHAEL S, GENENTECH INC, GOLDSTEIN JOSEPH L, MARSTERS JAMES C JR, REISS YUVAL, UNIV TEXAS, BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, GENENTECH, INC.
Application year: 1993
Main classification: C07K5
IPC codes: A61K38, A61P35, C07K1, C07K5, C07K7, C12N9, C12N15