REDUCTION OF HAIR GROWTH EMPLOYING SULFHYDRYL REACTIVE COMPOUNDS

Abstract: A method of reducing the rate of mammalian hair growth includes topically applying a composition containing a sulfhydryl reactive compound to the skin.

Description: -REDUCTION OF HAIR GROWTH EMPLOYING SULFHYDRYL REACTIVE COMPOUNDS' Reduction of Hair Growth The invention relates to reducing hair growth in mammals. Hair proteins include a fairly large quantity of the a ino acid cysteine, which includes a thiol (-SH) group. It is the formation of disulfide bonds between cysteine residues in the hair proteins, to form cystine, that give hair its strength and character. It is known in the art to use depilatory compositions to remove hair from, e.g., legs. Such compositions, when applied to the skin, digest the hair, in part, by breaking down the disulfide bonds in the hair. Such compositions typically include a chemical agent like calcium thioglycolate that aids the digestion process. We have discovered that the rate of mammalian (including human) hair growth can be reduced by applying a non-depilatory composition including sulfhydryl active compounds to the skin. Sulfhydryl active compounds, as used herein, are compounds that include a free -SH group, thiols without a free -SH group, and thiols or disulfides that can be converted to a moleculer with a free -SH group in cells. Non- depilatory, as used herein, is a composition which after a single topical application does not result in hair removal and/or degradation. Without being bound to any theory, it is believed that sulfhydryl active compounds reduce hair growth at least in part by one or more of the following mechanisms. During hair growth, cysteine is incorporated into protein chains. The -SH groups of cysteine residues in the protein chains form disulfide bonds (and cystine) , binding the protein chains together as part of the normal hair growth. Sulfhydryl active compounds, applied topically, penetrate the hair follicle and interfere with hair growth by (1) reacting with free cysteine to form a mixed cysteine-sulfhydryl active compound disulfide bond, resulting in there being less cysteine available for incorporation into disulfide bonds present in hair proteins; (2) reducing the disulfide bond in cystine in the hair proteins, at the same time forming a mixed cysteine-sulfhydryl active compound disulfide bond; and (3) reducing the disulfide bond in cystine, without concomitant formation of the mixed disulfide bond. Preferred sulfhydryl active compounds with a free -SH group include thiosalicylic acid, D-cysteine, 2-mercaptoethylamine (cysteamine) , captopril, N-acetyl-L-cysteine, cysteinylglycine, 2, 3-dimercapto-l- propanesulfonic acid, meso-2,3- dimercaptosuccinic acid, dimethylcysteamine, diethyldithiocarbamic acid, D-penicillamine, L- cysteine methyl ester, and -cysteine ethyl ester. Preferred sulfhydryl active compounds without a free -SH group include 3,3'- thiodipropionic acid, isethionic acid, 3- carboxypropyl disulfide, 3,3'-thiodipropionic acid dilauryl ester, sulfasalazine, 3- (inethythio) -propylamine, 5'-deoxy-5'- methylthioadenosine, allyl sulfide, DL-α-lipoic acid (reduced form), and D -methionine-S-methyl- sulfonium chloride. Preferred sulfhydryl active compounds that are converted to free thiols in cells include phosphocysteamine, which is dephosphorylated to cysteamine in cells; penicillamine disulfide, which is reduced to free penicillamine in cells; and S-2-aminoethyl- L-cysteine, which is hydrolyzed to cysteamine and serine (inactive) in cells. The sulfhydryl active compounds should not be of too high a molecular weight (greater than about 1000 daltons) , or contain highly charged phosphate groups, or compounds that may not adequately penetrate the skin. The composition contains, in addition to the sulfhydryl active compound, a non-toxic dermatologically acceptable vehicle or carrier which is adapted to be spread on the skin. The concentration of the compound may be varied over a wide range up to a saturated solution, preferably from 1% to 20% by weight. The reduction of hair growth increases as the amount of sulfhydryl active compound applied increases per unit area of skin; the maximum amount that can be effectively applied is limited primarily only by the rate at which the compound penetrates the skin. Generally, the effective amounts range from 100 to 2000 micrograms or more per square centimeter of skin. The following specific examples are intended to illustrate more clearly the nature of the present invention without acting as a limitation upon its scope. The inhibition in hair growth provided by compositions including the sulfhydryl active compounds was determined by following the Golden Syrian Hamster protocol, which is described in Shander et al., U.S. Pat. No. 5,132,293, Ahluwalia, U.S. Pat. No. 5,095,007 and Ahluwalia et al., U.S. Pat. No. 5,096,911. Four groups (eight animals in each group) of male intact Golden Syrian hamsters were provided. These animals were considered acceptable models for human beard hair growth in that they display oval shaped flank organs, one on each side, each about 8 mm. in major diameter, which grow thick black and coarse hair similar to human beard hair. These organs produce hair in response to androgens in the hamster. The flank organs of each hamster were depilated by applying a thioglycolate- based chemical depilatory (Surgex) , and to one organ of each animal was applied 10-25 mg. of vehicle alone once a day, while to the other organ of each animal was applied an equal amount of vehicle containing inhibitor. After three weeks of such applications (five days a week) , the flank organs were shaved and the amount of recovered hair (hair mass) from each was weighed.. The extent of reduction in hair growth was expressed as the percent decrease in hair mass on the organ treated with inhibitor as compared to the organ treated with vehicle alone. As a control, one group of eight animals had both flank organs of each animal treated with vehicle alone. The results were as shown in Table 1 below. Table 1 Inhibition of Hair Growth by Sulfhydryl Reactive Compounds Hair Mass Compound Dose Vehicle Treated m Untreated m Percent Reduction Thiosalicylic acid 2-Mercaptoethylamine (Cysteamine) L-Cysteine methyl ester L-Cysteine ethyl ester N-Acetyl-L-Cysteine 2,3, -Dimercapto-1-propanesulfonic e Dime hylaminoethanethiol Phosphocysteamine 3-Carboxypropyl disulfide 3,3' -Thiodipropionic acid Diethyldithiocarbamic acid D-Penicillamine Sulfasalazinβ D-Cysteine 5' -Deoxy-5' -methylthioadenosine Captopril DL-α-Lipoic acid (reduced form) Cysteinyl-glycine D-Penicillamine disulfide Isethionic acid meβo-2,3, -Dimercaptosuccinic acid 3,3' -Thiodipropionic acid dilauryl S-2-Aminoethyl- -cysteine 3,3' -Thiodipropionic acid dilauryl Table 1 (Continuation) Inhibition of Hair Growth by Sulfhydryl Reactive Compounds Hair Mass Compound 3- (Methylthio) -propylamine Allyl sulfide DL-α-Lipoic acid (reduced form) Vehicle A: 68% distilled H 2 0, 16% ethanol (100 proof), 5% propylene glycol, 5% dipropylene glycol, 4% benzyl alcohol, 2% propylene carbonate Vehicle B: 80% ethanol (190 proof), 17.5% distilled H 2 0, 2% propylene glycol dlpelargonate (Emerest 2388), 0.5% propylene glycol Vehicle C: Moisturizing lotion containing common cosmetic ingredients which include emulsifiers, detergents, and preservatives Vehicle D: 75% acetone, 20% propylene carbonate, 5% benzyl alcohol Vehicle E: 86% distilled H 2 0, 4% propylene glycol, 4% dipropylene glycol, 4% propylene carbonate, 2% ethanol The preferred compositions are those that provided a reduction in hair growth of at least 30%, and more preferably at least 50%, when tested according to the above procedure. The following biochemical properties of some of the sulfhydryl reactive compounds were tested: (1) the percent reduction in hair shaft cysteine caused by the compounds; (2) the ability of the compounds to form a cysteine- mixed disulfide in vitro; (3) the ability of the compound to form a cysteine-mixed disulfide in hair shafts; and (4) the ability of the compounds to reduce cystine. The percent reduction in hair shaft cysteine caused by the sulfhydryl reactive compounds was measured according to the following procedure. Amino acid analysis of hamster flank organ hairs was carried out using a commercially available amino acid analysis system (Pico-Tag system, available from Waters Associates, Inc., Milford, MA). The hairs were thoroughly washed, then hydrolyzed by HCL vapors at 115°C. overnight. The hydrolyzed hairs (now free amino acids) were derivatized with phenylisothiocyanate to yield the phenylthiohydantion derivatives of the respective amino acids, which were then separated by C-18 reverse phase chromatography (HPLC) , and quantitated by an in-line UV spectrophotometer. It is believed that the reduction of cysteine levels in hair shafts caused by some of the sulfhydryl active compounds is at least in part responsible for the reduction in hair growth caused by these compounds. The ability of the sulfhydryl reactive compounds to form cysteine-mixed disulfides in hair shafts was determined according to the following procedure. Groups of eight (8) Golden Syrian hamsters were treated topically with a sulfhydryl active compound on one flank organ (treated site) and the carrier vehicle without the sulfhydryl active compound on the other flank organ (control site) . The carrier vehicles were the same as for the results achieved in Table 1. Following thirteen (13) treatments (Mon-Fri, over 18 days) , hair shafts from the treated flank organs were harvested and analyzed for the presence of cysteine-mixed disulphides. It is believed that the ability of some of the sulfhydryl reactive compounds to form the cysteine-mixed disulfides in the hair shaft is at least in part responsible for the reduction in hair growth caused by these compounds, as the hair shaft proteins fail to undergo final post-translational maturation (disulfide formation) . The ability of the sulfhydryl reactive compounds to form cysteine-mixed disulfides in vitro was determined by incubating the sulfhydryl reactive compounds in test tubes, with either cystine or cysteine, under physiological conditions (i.e. pH 7.4 and at a temperature of 37°C.) . The reaction of these compounds with cysteine or cystine was evaluated by HPLC analysis. It is believed that the ability of a sulfhydryl reactive compound to form a cysteine-mixed disulfide in vitro provides an indication that the compound is capable of forming cysteine-mixed disulfides with free cysteine present in hair follicle bulbs prior to cysteine incorporation into protein of the hair shaft when applied topically to the skin. The ability of sulfhydryl reactive compounds to reduce cystine was determined by incubating the respective sulfhydryl compound with cystine at physiological conditions of temperature and pH (37°C, pH 7.4). Following the incubation, the samples were derivatized and analyzed on HPLC as given above. For cysteamine, phosphocysteamine and dimethylcysteamine the samples were analyzed without derivatization, using an electrochemical detector instead of the UV detector used in amino acid analysis. The determination of cystine reduction by the compounds was based on generation of cysteine (free thiol) in the incubation mixture. It is believed that reducing the disulfide bond in cystine in hair proteins results in reduced hair growth. The results of the testing of these properties are recorded in Table 2. Table 2 Biochemical Properties of Select Sulfhydryl Reactive Agents Formation of C steine Sulfhydryl reactive agent Percent reduction in hair shaft cysteine D-Penicillamine 50% Cysteamine 50% Dimethyl cysteamine 28% Phospho cysteamine 24% Dimercaptopropanesulfonic acid 40% Meso-dimercaptosuccinic acid 22% Captopril 26% 5'-Deoxy methylthioadenosine 11% Diethyl dithiocarbamic acid 18% Thiosalicylic acid 14% Sulfasalazinβ (-2%) Cysteinyl-glycine ND* α-Lipoic acid ND* ND*: Not Determined

Claims: Other embodiments are within the claims. C L A I M S 1. , A process of reducing the rate of mammalian hair growth, comprising applying a non-depilatory composition including an effective amount of a sulfhydryl active compound to the skin, said compound reducing the rate of hair growth from said skin. 2. The process of claim 1, wherein after application said compound penetrates into the hair follicles in said skin and reacts with free ■ cysteine in said hair follicle cells to form cysteine-mixed disulfides. 3. The process of claim 1, wherein said sulfhydryl active compound after application penetrates into the pre-ketanized hair shafts in said skin and reduce the disulfide bond in cystine in hair proteins. 4. The process of claim 3, wherein said sulfhydryl active compound also forms a mixed disulfide bond with one of the cysteine moieties in hair shaft proteins. 5. The process of claim 1, wherein said sulfhydryl active compound is cysteamine. 6. The process of claim 1, wherein said sulfhydryl active compound is D-penicillamine. 7. The process of claim 1, wherein said sulfhydryl active compound is dimethyl cysteamine. 8. The process of claim 1, wherein said sulfhydryl active compound is phosphocysteamine. 9. The process of claim 1, wherein said sulfhydryl active compound is captopril. 10. The process of claim 1, wherein said sulfhydryl active compound is meso- dimercaptosuccinic acid. 11. The process of claim 1, wherein said sulfhydryl active compound is diethyldithiocarbamic acid. 12. The process of claim 1, wherein said sulfhydryl active compound is cysteinyl-glycine. 13. The process of claim 1, wherein said sulfhydryl active compound is D-cysteine. 14. The process of claim 1, wherein said sulfhydryl active compound is N-acetyl-cysteine. 15. The process of claim 1, wherein said sulfhydryl active compound is thiosalicylic acid. 16. The process of claim 1, wherein said sulfhydryl active compound is lipoic acid. 17. The process of claim 1, wherein said sulfhydryl active compound is 5'-deoxy-5~- methyl-thioadenosine. 18. The process of claim 1, wherein said sulfhydryl active compound is L-cysteine methyl ester. 19. The process of claim 1, wherein said sulfhydryl active compound is sulfasalazine. 20. The process of claim 1, wherein said sulfhydryl active compound is L-cysteine ethyl ester. 21. The process of claim 1, wherein said sulfhydryl active compound is 3-carboxypropyl disulfide. 22. The process of claim 1, wherein said sulfhydryl active compound is applied to the face. 23. The process of claim 1, wherein said sulfhydryl active compound has a free -SH group. 24. The process of claim 1, wherein said sulfhydryl active compound is a thiol without a free -SH group. 25. The process of claim 1, wherein said sulfhydryl active compound is a thiol or disulfide that can be converted to a molecule with a free -SH group in cells. 26. The process of claim 1, wherein said composition reduces hair growth by at least 30% when tested according to the Golden Syrian Hamster protocol. 27. The process of claim 1, wherein said composition reduces hair growth by at least 50% when tested according to the Golden Syrian Hamster protocol.

Inventors: AHLUWALIA GURPREET S, MARKS-DEL GROSSO DIANA, SHANDER DOUGLAS, AHLUWALIA, GURPREET, S., MARKS-DEL GROSSO, DIANA, SHANDER, DOUGLAS
Applicants: AHLUWALIA GURPREET S, HANDELMAN JOSEPH H, MARKS DEL GROSSO DIANA, SHANDER DOUGLAS, AHLUWALIA, GURPREET, S., HANDELMAN, JOSEPH, H., MARKS-DEL GROSSO, DIANA, SHANDER, DOUGLAS
Application year: 1993
Main classification: A61K31
IPC codes: A61K8, A61K31, A61P17, A61P43, A61Q5, A61Q7, A61Q9, C07D207, C07D339