Use of four K-252a derivatives

Abstract: Derivatives of K-252a such as (II)-(IV) are useful for treating diseased neuronal cells.

Description: Background of the Invention Protein kinases are a broad class of enzymes which act to modify chemically many cellular proteins, by phosphorylation of amino acids. Inhibitors of protein kinases are structurally varied, and have variable (and sometimes contradictory) effects on the nervous system and other tissues. A given protein kinase inhibitor may influence more than one protein kinase. For example, K-252a, an alkaloid-like material isolated from the culture broth of Nocardiopsis sp. and Actinomadula sp. was originally reported to be a protein kinase C inhibitor, but was subsequently found also to inhibit protein kinases A and G, myosin light-chain kinase, and trk (a tyrosine kinase activated by nerve growth factor [NGF], the latter a neurotrophic protein which promotes the survival of peripheral, sensory and sympathetic neurons). Consistent with this latter effect, K-252a blocks the neurotrophic actions of NGF on PC-12 cells (chromaffin cells from rat adrenal medullary tumors, pheochromocytomas), and promotes the survival of dorsal root ganglion neurons and hippocampal neurons. However, it has been found to be cytotoxic at a wide range of concentrations, leading some investigators to conclude that it has limited usefulness in vivo . A microbial alkaloid related to K-252a, staurosporine, also has a variety of effects on different protein kinases and cell types. Staurosporine was found to have NGF-like effects on PC-12 cells, and to protect the gerbil hippocampus from post-ischemic injury. It is able to reverse damage to cholinergic neurons in the rat basal forebrain. K-252a and staurosporine have been proposed as tumor inhibitors. Staurosporine has been offered as an insecticide. Derivatives of staurosporine, with a hydrocarbyl radical or an acyl radical substituted at the methylamine nitrogen, have been made and proposed for the following uses: tumor inhibition, inflammation inhibition, immunomodulation, and treatment of diseases of the cardiovascular and central nervous systems. Borasio, Neurscience Letters, Vol. 108, No. 1-2, pages 207-212 (1990), discloses the differential effects of K-252a on the in vitro survival of chick embryonic neurons. Summary of the Invention The present invention is the use of a compound for the manufacture of a medicament for use in enhancing, in a mammal, the function of a dorsal root ganglion neuron, the compound being represented by the formula wherein the following substitutions are made: Compound R 1 R 2 X R II-4 H H CH 2 OH OCH 3 II-12 H H CH 2 OH OH II-15 H H CH 2 SOCH 3 OH II-51 CH 2 SC 2 H 5 CH 2 SC 2 H 5 CO 2 CH 3 OH Description of the invention The present invention relates to the use of bis-N-substituted derivatives of staurosporine as therapeutics for neurological diseases, especially those diseases characterized either by neuronal cells which are injured, compromised, undergoing axonal degeneration, or at increased risk of dying, or by impaired cholinergic activity. These diseases include those induced by excitatory amino acids. The present invention is preferably used in the treatment of Huntington's disease. Therapy according to the invention includes use of the derivatives alone and use of the derivatives combined with exogenous administration of a neurotrophic factor, preferably a member of the neurotrophic family, and most preferably nerve growth factor (NGF). The neurotrophic family is a group of proteins with significant homology to NGF and includes, in addition to NGF, brain-derived neurotrophic factor (BDNF; Leibrok et al ., Nature 341 :149-152, 1989); neutrophil-3 (NT-3; Hohn et al. , Nature 344 :339-341, 1990); and neurotrophic-5 (NT-5; Berkemeier et al ., Neuron 7 :857-866, 1991). Compounds for use in the invention may be prepared de novo by chemical synthesis using methods known to those skilled in the art. For example, procedures used for preparation of the compounds are described in US-A-4923986. Further, methods are described for compounds II-12 and II-15 in Japanese Patent Application No. 60-295173 (1985); and for compound II-4 in Japanese Patent Application No. 62-327858 (1987). The bioactivity of compounds of the invention, including combination with a neurotrophic factor, may conveniently be assayed by a cultured dorsal root ganglion neuron survival assay, as described in detail below. Thus, the compounds of this invention are useful for administration to humans or other mammals who suffer from neurological diseases or disturbances characterized by increased risk of neuronal cell death or dysfunction, as described above. These neurological diseases and disturbances include but are not limited to: Alzheimer's disease; motor neuron disease including amyotrophic lateral sclerosis; Parkinson's disease; stroke or other ischemic injuries; Huntington's disease; AIDS dementia; epilepsy; concussive or penetrating injuries of the brain or spinal cord; and peripheral neuropathies. The compounds provided herein can be formulated into pharmaceutical compositions by admixture with pharmaceutically acceptable nontoxic excipients and carriers. As noted above, such compositions may be prepared for use in parenteral administration, particularly in the form of liquid solutions or suspensions; for oral administration, particularly in the form of tablets or capsules; or intranasally, particularly in the form of powders, nasal drops, or aerosols. The composition may conveniently be administered in unit dosage form and may be prepared by any of the methods well known in the pharmaceutical art, for example, as described in Remington's Pharmaceutical Sciences (Mack Pub. Co, Easton, PA, 1980). Formulations for parenteral administration may contain as common excipients sterile water or saline, polyalkylene glycols such as polyethylene glycol, oils of vegetable origin, hydrogenated naphthalenes and the like. In particular, biocompatable, biodegradable lactide polymer, lactide/glycolide copolymer, or polyoxyethylenepolyoxypropylene copolymers may be useful excipients to control the release of the active compounds. Other potentially useful parenteral delivery systems for these active compounds include ethylene-vinyl acetate copolymer particles, osmotic pumps, implantable infusion systems, and liposomes. Formulations for inhalation administration contain as excipients, for example, lactose, or may be aqueous solutions containing, for example, polyoxyethylene-9-lauryl ether, glycocholate and deoxycholate, or oily solutions for administration in the form of nasal drops, or as a gel to be applied intranasally. Formulations for parenteral administration may also include glycocholate for buccal administration, methoxysalicylate for rectal administration, or citric acid for vaginal administration. The materials of this invention can be employed as the sole active agent in a pharmaceutical or can be used in combination with other active ingredients, e.g., other growth factors which could facilitate neuronal survival or axonal growth in neurological diseases or disorders, for example, peripheral neuropathy. The concentrations of the compounds described herein in a therapeutic composition will vary depending upon a number of factors, including the dosage of the drug to be administered, the chemical characteristics (e.g., hydrophobicity) of the compounds employed, and the route of administration. In general terms, the compounds of this invention may be provided in an aqueous physiological buffer solution containing about 0.1 to 10% w/v compound for parenteral administration. Typical dose ranges are from about 1 µg/kg to about 1 g/kg of body weight per day; a preferred dose range is from about 0.01 mg/kg to 100 mg/kg of body weight per day. The preferred dosage of drug to be administered is likely to depend on such variables as the type and extent of progression of the neurological disease, the overall health status of the particular patient, the relative biological efficacy of the compound selected, the formulation of the compound excipients, and its route of administration. The present invention will be further illustrated by the following Examples. Example 1 Compound II-4 Compound A (962 mg, 2 mmol) was dissolved in a mixture of 30 ml of tetrahydrofuran and 10 ml of methanol, and then 760 mg of sodium borohydride (20 mmol) was added thereto under ice cooling, followed by stirring at the same temperature for 4 hours and further at room temperature for 12 hours. After 3N hydrochloric acid was added thereto, the solution was washed with an aqueous solution of sodium chloride and dried over magnesium sulfate, followed by evaporation of the solvent. The residue was purified by silica gel column chromatography (chloroform/methanol = 98/2) to give 882 mg (yield 97%) of Compound II-4. Melting Point: 130-140°C 1 H-NMR (DMSO-d 6 ) δ (ppm): 2.032(1H, dd, J=5.0, 13.9Hz), 2.231 (3H, s), 2.967(3H, s), 3.609(1H, dd, J=7.6, 13.4Hz), 3.959(2H, m), 5.000(2H, s), 5.268(1H, t, J=5.3Hz), 7.065(1H, dd, J=4.9, 7.3Hz), 7.254-8.038 (7H, m), 8.565(1H, s), 9.206(1H, d, J=7.8Hz) Example 2 Compounds II-51 (see Fig.1) Compound (E) (Japanese Published Unexamined Patent Application No. 295588/88; supra) (60.7 mg, 0.1 mmol) was dissolved in a mixture of 5 ml of chloroform and 1 ml of methanol, and then 11 mg (0.3 mmol) of sodium borohydride was added thereto under ice cooling, followed by stirring at the same temperature for 15 minutes. After dilution with chloroform, the mixture was washed successively with water and a saline solution, and dried over potassium carbonate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography (Chloroform/methanol/triethylamine = 98/2/0.5) to give 36 mg (yield 59%) of Compound (F). 1 H-NMR (DMSO-d 6 ) δ (ppm): 1.650(3H, s), 2.027(1H, dd, J=4.9, 14.5Hz), 2.126(3H, s), 3.843(1H, dd, J=7.4, 14.5Hz), 3.891(3H, s), 4.607(2H, s), 4.673(2H, s), 5.125(2H, s), 7.099(1H, dd, J=5.0, 7.3Hz), 7.437-7.907(5H, m), 8.812(1H, d, J=0.8Hz) FAB-MS (m/z): 612 (M+1) + Compound (F) (159 mg, 0.26 mmol) was dissolved in 15 ml of chloroform, and then 0.8 ml (10.4 mmol) of ethanethiol and 24 mg (0.104 mmol) of camphorsulfonic acid were added thereto, followed by stirring at room temperature for 12 hours. The solution was washed successively with a saturated aqueous solution of sodium bicarbonate, water, and a saline solution, and dried over sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography (ethyl acetate/toluene = 1/9 - chloroform/methanol = 99/1) to give 43 mg of Compound (G) and 75 mg of Compound (H). Compound (G) 1 H-NMR (CDCl 3 ) δ (ppm): 1.292(3H, t, J=7.4Hz), 1.297 (3H, t, J=7.4HZ), 1.799(3H, s), 2.141(1H, dd, J=5.0, 14.5Hz), 2.256(3H, s), 2.532(2H, q, J=7.4Hz), 2.553(2H, q, J=7.4Hz), 2.869(3H, s), 3.971(1H, dd, J=7.5, 14.5Hz), 3.992(2H, s), 4.005 (3H, s), 4.021(2H, s), 5.416(1H, dd, J=17.5Hz), 5.459(1H, d, J=17.4Hz), 6.989(1H, dd, J=5.1, 7.4Hz), 7.509-7.963(5H, m), 9.134(1H, d, J=1.2Hz) FAB-MS (m/z): 700 (M+1) + Compound (H) 1 H-NMR (CDCl 3 ) δ (ppm): 1.294(3H, t, J=7.4Hz), 1.799(3H, s), 2.149(1H, dd, J=5.0, 14.6Hz), 2.273(3H, s), 2.533(2H, q, J=7.4Hz), 2.813 (3H, s), 3.972(1H, dd, J=7.4, 14.6Hz), 4.008(3H, s), 4.015(2H, s), 4.951 (2H, s), 5.377(1H, d, J=17.4Hz), 5.418(1H, d, J=17.4Hz), 6.973(1H, dd, J=5.0, 7.5Hz), 7.481-8.037 (5H, m), 9.093(1H, d, J=1.2Hz) FAB-MS (m/z): 656 (M+1) + Substantially the same procedure as follows was carried out using 34 mg of compound (G) to give 18.7 mg of compound II-51 : The Starting Compound (0.012 mmol) was dissolved in a mixture of 0.2 ml of tetrahydrofuran and 0.2 ml of methanol, and then 2 µl of 28% sodium methoxide/methanol was added thereto, followed by stirring at room temperature for 10 minutes. To the solution was added 0.1 ml of a 5% aqueous solution of citric acid, followed by dilution with chloroform. The mixture was washed successively with water and a saline solution, and dried over sodium sulfate. After evaporation of the solvent, the residue was subjected to silica gel column chromatography (chloroform/methanol = 9/1) to give the desired product. 1 H-NMR (CDCl 3 ) δ (ppm): 1.300(3H, t, J=7.4Hz), 1.325(3H, t, J=7.4Hz), 2.185(3H, s), 2.514(1H, dd, J=4.8, 14.5Hz), 2.540(2H, q, J=7.4Hz), 2.555(2H, q, J=7.4Hz), 3.384(1H, dd, J=7.5, 14.5Hz), 3.941(2H, s), 3.976(2H, s), 4.094(3H, s), 4.836(1H, d, J=16.4Hz), 4.910(1H, d, J=16.3Hz), 5.781 (1H, s),6.845 (1H, dd, J=4.8, 7.5Hz), 7.371-7.843(5H, m),8.998(1H, s) FAB-MS (m/z): 616 (M+1) + Example 3 Compounds were assessed for their ability to promote dorsal root ganglion neuron cell survival. Cell survival was measured by uptake of calcein AM, an analogue of the viable dye, fluorescein diacetate. Calcein is taken up by viable cells and cleaved intracellularly to fluorescent salts which are retained by intact membranes of viable cells. Microscopic counts of viable neurons correlate directly with relative fluorescence values obtained with the fluorimetric viability assay. This method thus provides a reliable and quantitive measurement of cell survival in the total cell population of a given culture. Dorsal root ganglion neuron survival was enhanced by K-252a in a concentration-dependent manner; maximum activity was observed at approximately 100 nM. Other tested compounds were active in promoting DRG neuron survival, as shown in Table 1 below. All of these analogues were also active in increasing spinal cord ChAT activity. Microscopic examination of the dorsal root ganglion neurons stimulated with the compounds indicated enhanced nerve fiber outgrowth as well. Methods: Dorsal root ganglia were dissected from embryonic age day 8 chick embryos and dissociated cells prepared by subsequent Dispase (neutral protease, Collaborative Research) dissociation. Neurons were seeded at low density (1.8 x 10 4 cells/cm 2 ) into 96 well poly-L-ornithine and laminin-coated plates. Cells were cultured for 48 hours in serum-free N2 medium (Bottenstein and Sato, 1979) at 37°C in a humidified atmosphere, 5% CO 2 /95% air. Cell survival was assessed at 48 hours using the viable fluorimetric assay described above. Compound at 300 nM DRG Survival % K-252a % NGT II-15 105 158 II-12 83 125 II-4 83 95

Claims: Use of a compound for the manufacture of a medicament for use in enhancing, in a mammal, the function of a dorsal root ganglion neuron, the compound being represented by the formula wherein the following substitutions are made: Compound R 1 R 2 X R II-4 H H CH 2 OH OCH 3 II-12 H H CH 2 OH OH II-15 H H CH 2 SOCH 3 OH II-51 CH 2 SC 2 H 5 CH 2 SC 2 H 5 CO 2 CH 3 OH Use according to claim 1, wherein the compound is selected from II-4, II-12 and II-51. Use according to claim 1 or claim 2, wherein the medicament additionally comprises a neurotrophic factor. Use according to claim 3, wherein the neurotrophic factor is a member of the neurotrophin family. Use according to claim 4, wherein said member is nerve growth factor (NGF). Use according to any preceding claim, for use in the treatment of Huntingdon's disease.

Inventors: GLICKSMAN MARCIE A, KAUER JAMES C, LEWIS MICHAEL E, MATSUDA YUZURU, MURAKATA CHIKARA, NEFF NICOLA, ROBERTS-LEWIS JILL, SAITO HIROMITSU, GLICKSMAN, MARCIE A., KAUER, JAMES C., LEWIS, MICHAEL E., MATSUDA, YUZURU, MURAKATA, CHIKARA, NEFF, NICOLA, ROBERTS-LEWIS, JILL, SAITO, HIROMITSU
Applicants: CEPHALON INC, KYOWA HAKKO KOGYO KK, CEPHALON, INC., KYOWA HAKKO KOGYO CO., LTD.
Application year: 1993
Main classification: A61K31
IPC codes: A61K31, C07D519, C07D487, A61P25, C07D498