INSECT VIRUSES AND THEIR USES IN PROTECTING PLANTS

Abstract: The present invention relates to an isolated small RNA virus capable of infecting insect species including Heliothis species, and to the nucleotide sequences and proteins incoded thereby. The invention contemplates uses of the virus in controlling insect attack in plants.

Description: INSECT VIRUSES AND THEIR USES IN PROTECTING PLANTS FIELD OF THE INVENTION The present invention relates to insect viruses useful in control of insect attack on plants. It particularly relates to biological insecticides, especially those comprised of insect viruses. In particular applications, the invention also provides recombinant viruses and transgenic plants. BACKGROUND OF THE INVENTION There is increasing awareness of the desirability of insect pest control by biological agents. Considerable effort in recent years has been devoted to the identification and exploitation of DNA viruses with large genomes, especially the baculoviruses. These viruses generally require extensive genetic manipulation to become effective insecticides, and the first such modified viruses are only now being evaluated. In contrast, very little effort has been devoted to the study and use of small viruses with RNA genomes. Four main groups of small RNA viruses have been isolated from insects. These include members of the picomaviridae, the Nodaviridae, the tetraviridae and the unclassified viruses. Descriptions of these groups can be found in the Adas of Invertebrate Viruses (eds J.R. Adams and J. R. Bonami) (CRC Press, Boca Raton, 1991) and Viruses of Invertebrates (ed. E. Kurstak) (Marcel Dekker, New York, 1991). These disclosures relating to these viruses concern their pathology and biology, not their use in biological control. SUMMARY OF THE INVENTION In a first aspect of the present invention there is provided an isolated small RNA virus capable of infecting insect species including Heliothis species. ) SUBSTITUTE SHEET In one particular embodiment, the present invention provides an isolated preparation of Heliothis armigera stunt virus referred to as "HaSV" herein. In a further aspect of the present invention there is provided an isolated nucleic acid molecule comprising a nucleic acid sequence hybridizable with RNA 1 or RNA 2 described herein under low stringency conditions. In still a further aspect the invention provides a vector comprising a nucleic acid molecule, the sequence of which is hybridizable with RNA 1 or RNA 2 as described herein. These vectors include expression and transfer vectors for use in animals including insect, plant and bacterial cells. In a further aspect the invention provides an isolated protein or polypeptide preparation of the proteins or polypeptides derivable from the isolated virus of the present invention. The invention also extends to antibodies specific for the protein and polypeptide preparations. In a yet further aspect the invention provides a recombinant insect virus vector incorporating all or a part of the isolated virus of the present invention. In a still further aspect of the present invention there is provided a method of controlling insect attack in a plant comprising genetically manipulating said plant so that it is capable of expressing HaSV or mutants, derivatives or variants thereof or an insecticidally effective portion of HaSV, mutants, variants or derivatives thereof and optionally other insecticidally effective agents such that insects feeding on the plants are deleteriously effected. In another aspect of the present invention there is provided a preparation of HaSV or a mutant variant or derivative thereof, or an insecticidally effective portion of HaSV, mutant, variant or derivative thereof, suitable for application to plants, wherein the preparation is capable of imparting an insect protective effect. I SUBSTITUTE SHEET BRIEF DESCRIPTION OF FIGURES Figure 1 is the complete sequence of RNA 1 and of major encoded polypeptide. Figure 2 is the complete sequence of RNA 2 in the authentic version, and its encoded polypeptides (the RNA 2 variant called the "5C version" is also shown around nucleotide position 570 [the amino acid sequence encoded by the 5C version is not included but this may be deduced from the nucleotide sequence given]). Figure 3 is bioassay data showing HaSV-induced stunting of larvae. Figure 4 is a schematic representation of the proteins encoded by RNA 1 and RNA 2. Figure 5 is a schematic representation of the proteins expressed by RNA 2 in bacteria DNA fragments encoding P17, P71, P64, P7 and the fusion protein P70 were synthesised by PCR. The flanking Ndel and BamHI sites used in cloning are indicated. (Note that P17 is followed by a Bglll site, whose cohesive ends are compatible with those of BamHI). Figure 6 illustrates the 3'-teπninal secondary structure of HaSV RNAs. The tRNA-like structures at the 3' ends of RNAs 1 and 2 are shown. Residues in bold are common to both sequences. Figure 7 Expression strategies for HaSV cDNAs in insect cells. The upper part of the figure shows the genome organization of RNAs 1 and 2. The lower part shows insertion of cDNAs corresponding to these RNAs into a plasmid vector, between the heat shock protein (HSP70) promoter of Drosophila and a suitable polyadenylation (pA) signal. The HSP promoter was obtained by PCR using suitable primers, with a BaMHI site inserted by PCR immediately upstream of the start of transcription, giving the following sequence: GGATCCACAGnnn, where the underlined residue is the transcription start site for either RNA. The cDNAs are termined by Clal sites, allowing direct linkage to ribozyme sequences as described in the text. Figure 8 Ribozymes to yield correct 3' ends. The sequences of the ribozymes inserted as short cDNA fragments into HaSV cDNA clones are shown. The I SUBSTITUTE SHEET ribozyme fragments were assembled and cloned as described in the text. Designed self-cleavage points are indicated by bold arrows. Figure 9 Immunoblots to map epitopes on HaSV. A. Detected with HaSV antiserum. Lane 1: P TP70delSP; lane 2: P TP70; lane 3: P TP17; lane 4: control; lane 5: P TP70delN; lane 6: pTP70; lane 7: pTP71; lane 8: HaSV virions; lane 9: molecular weight markers. B. Detected with HaSV antiserum. Lane 1: P TP70delN; lane 2: P TP70delSPN; lane 3: pTP70. C. Detected with an antiserum to the Bt toxin (CryIA(c)). lane 1: pTP70; lane 2: HaSV virions; lane 3: control extract. Figure 10 New field isolates of HaSV. The genomic organization of RNA 2 is shown at the top of the Figure. PCR using appropriate primers with BamHI restriction sites and in some cases altered context sequences of the AUG initiating translation of the P17 or P71 genes were used to make fragments for cloning into the BamHI sites of the expression vectors. Constructs 17E71 and P71 have altered context sequences of the AUG initiating translation of the P17 and P71 genes respectively, these alterations correspond to the context derived from the JHE gene (see text). All context sequences are given on the right of the figure. R2 is a clone of the complete RNA sequence as a BamHI fragment in the vector. Figure 11 Maps of the expression constructs in baculovirus vectors. Figure 12 a to e Various strategies utilising the present invention. Figure 13 Expression of RNAs 1 and 2 from baculovirus vectors. The full- length cDNA clone of HaSV RNA 1 or 2 was inserted as a BamHI fragment into the baculoexpression vectors. PCR was used to add BamHI sites immediately adjacent to the 5' and 3' termini of the RNA 1 sequence; sequences of the primers are given in the text. Constructs R1RZ and R2RZ carry cis-acting ribozymes immediately adjacent to the 3' end of the sequence of RNA 1 and 2 respectively. Figure 14 Expression strategies for HaSV cDNAs in plant cells. The upper part of the Figure shows the genome organization of RNAs 1 and 2. The lower part shows insertion of cDNAs corresponding to these RNAs into a plasmid vector, between 35S promoter of cauliflower mosaic virus and the SUBSTITUTE SHEET polyadenylation (pA) signal on plasmid pDH51 (Pietrzak et al, 1986). The cDNAs were obtained by PCR using suitable primers, with a BaMHI site inserted by PCR immediately upstream of the start of each cDNA. The cDNAs are terminated by Clal sites, allowing direct linkage to ribozyme sequences as described in die text. DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS A first aspect of the invention contemplates use of small RNA viruses for biological control of insects. In particular, in accordance with the first aspect of this invention there is provided an isolated small RNA virus, particularly H. armigera stunt virus or mutants, variants or derivatives thereof capable of infecting insects, in particular the insect species such as Helicoveψa armigera. The small RNA virus isolate of the instant invention is insecticidal and in particular stunts the growth of insect larvae, for example Helicoverpa armigera larvae and inhibits or prevents development into die adult stage. The small RNA viruses of the instant invention have insecticidal, anti-feeding, gut-binding or any synergistic property or other activity useful for insect control. In particular, Helicoverpa armigera stunt virus (HaSV) particles are isometric and approximately 36 nm in diameter with a buoyant density on CsCl gradients of 1.36g/ml. The virus is composed of two major capsid proteins of approximately 64 and 7 KDa in size as determined on SDS-PAGE. The HaSV genome is much later than the largest known nodavirus (another class of RNA viruses) and comprises two ss ( + ) RNA molecules of approximately 5.3 and 2.4 kb. The genome appears to lack a blockage of unknown structure at the 3' termini that is found in Nodaviridae. The HaSV genome however shares a capped structure and non-polyadenylation with Nodaviridae. HaSV differs significantly from Nodaviridae and Nudaurelia ω virus in terms of its immunological properties. In particular the large capsid protein has different SUBSTITUTE SHEET 466 - 6 - antigenic determinants. Other properties of HaSV are described in the Examples. The host range of HaSV includes Lepidopterans such as from the subfamily Heliothinae. Species known to be hosts are Helicoverpa (Heliothis) armigera, H. punctigera, H. zea, Heliothis virescens and other such noctuides as Spodoptera exigua. H. armigeravMch is known by the common names corn ear worm, cotton ball worm, tomato grub and tobacco bud worm is a pest of economic significance in most countries. Hpunctigera, the native bud worm, is a pests of the great economic significance in Australia. Members of the Heliothinae, which include Helicoveφa and Heliothis, and especially Harmigera are among the most important and widespread pests in the world. In the US Heliothis virescens and Helicoverpa zea are particularly important pests. The first aspect of the invention provides an isolated small RNA virus capable of infecting insects including Heliothis species. In a particularly preferred form die invention relates to mutants, variants and derivatives of HaSV. The terms "mutant", 'Variant and "derivative" include all naturally occurring and artificially created viruses or viral components which differ from the HaSV isolate as herein described in nucleotide content or sequence, amino acid content or sequence, immunological reactivity, non-glycosylation or glycosylation pattern and/or infectivity but generally retain insecticidal activity. Specifically the terms "mutant", 'Variant" and "derivative" of HaSV covers small RNA viruses which have one or more functional characteristic of HaSV described herein. Examples of mutants, variants or derivatives of HaSV include small RNA viruses that have different nucleic or amino acid sequences from HaSV but retain one of more functional features of HaSV. These may include strains witii genetically silent substitutions, strains carrying replication and encapsidation sequences and signals that are functionally related to HaSV, or strains that carry functionally related protein domains. SUBSTITUTE SHEET In a preferred aspect the invention relates to mutants, variants or derivatives 2of HaSV which encode replication or encapsidation sequences, structures or signals with 60%, preferably 70%, more preferably 80%, still more preferably 90% and even more preferably 95% nucleotide sequence identity to the nucleotide sequences HaSV. In another preferred aspect the invention relates to mutants, variants or derivatives of HaSV which encode proteins with at least 50%, preferably 60%, preferably 70%, more preferably 80%, still more preferably 90% and even more preferably 95% amino acid sequence identity to proteins or polypeptides of HaSV. In another preferred aspect the invention relates to mutants, variants or derivatives of HaSV with 50%, more preferably 60%, still more preferably 70%, more preferably 80%, still more preferably 90 or 95% nucleotide sequence identity to d e following biologically active domains encoded by die HaSV genome: RNA 1 - amino acid residues 401 to 600 or the other domains in the replicase RNA 2 (in the capsid protein) amino acid residues 273 to 435 amino acid residues 50 to 272 amino acid residues 436 to die COOH terminus Preferably the viral isolate of the present invention is biologically pure which means a preparation of die virus comprising at least 20% relative to otiier components as determined by weight, viral activity or any other convenient means. More preferably the isolates are 50% pure, still more preferably it is 60%, even more preferably it is 70% pure, still more preferably it is 80% pure and even more preferably it is 90% or more, pure. SUBSTITUTE SHEET In a second aspect the present invention relates to a nucleotide sequence or sequences hybridizable with those of HaSV. The term nucleotide sequence used herein includes RNA, DNA, cDNA and nucleotide sequences complementary thereto. Such nucleotide sequences also include single or double stranded nucleic acid molecules and linear and covalentiy closed circular molecules. The nucleic acid sequences may be d e same as the HaSV sequences as herein described or may contain single or multiple nucleotide substitutions and/or deletions and/or additions tiiereto. The term nucleotide sequence also includes sequences with sufficient homology to hybridize with the nucleotide sequence under low, preferably medium and most preferably high stringency conditions (Sambrook J, Fritsch, E.F. & Maniatis T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition, Cold Spring Harbour Laboratories Press) and to nucleotide sequences encoding functionally equivalent sequences. In still a more preferred embodiment die invention comprises the nucleotide sequences of genome components 1 and 2 as represented by Figures 1 and 2 hereinafter or parts thereof, or mutants, variants, or derivatives tiiereof. The terms "mutants", 'Variants" or "derivatives" of nucleotide genome components 1 and 2 has die same meaning, when applied to nucleotide sequences as tiiat given above and includes parts of genome components 1 and 2. The second aspect of the invention also relates to nucleotide signals, sequences or structures which enable the nucleic acid on which tiiey are present to be replicated by HaSV replicase. Furthermore the invention relates to die nucleotide signals, sequences or structures which enable nucleic acids on which tiiey are present to be encapsidated. In a particularly preferred embodiment of die second aspect, the invention comprises nucleotide sequences which are mutants of the capsid gene having the following sequences: ATG GGC GAT GCC GGC GTC GCGT TCA CAG SUBSTITUTE SHEET ATGGAG GATGCTGGAGTGGCGTCACAG ATGAGCGAGGCCGGCGTCGCGTCACAG In a preferred aspect the invention relates to nucleotide sequences of HaSV encoding insecticidal activity including the capsid protein gene and P17 and mutants, variants and derivatives thereof. In another preferred aspect the invention comprises nucleotide sequences including die following ribozyme oligonucleotides: 5'CCATCGATGCCGGACTGGTATCCCAGGGGG (called "HVRlCla" herein) 5' CCATCGATGCCGGACTGGTATCCCGAGGGAC (called "5ΗVR2Cla" herein) 5' CCATCGATGATCCAGCCTCCTCGCGGCGCCGGATGGGCA (called "RZHDV1" herein) 5' GCTCTAGATCCATTCGCCATCCGAAGATGCCCATCCGGC (called "RZHDV2" herein) 5' CCATCGATTTATGCCGAGAAGGTAACCAGAGAAACACAC (called "RZHC1" herein) 5' GCTCTAGACCAGGTAATATACCACAACGTGTGTTTCTCT (called "RZHC2" herein) Ribozyme sequences are useful for obtaining translation, replication and encapsidation of the transcript. It is therefore desirable to cleave the transcript downstream of its t-RNA-like structure or poly A tail prior to translation, replication or encapsidation of the transcript. SUBSTITUTE SHEET The present invention also further extends to oligonucleotide primers for the above sequences, antisense sequences and nucleotide probes for die above sequences and homologues and analogues of said primers, antisense sequences and probes. Such primers and probes are useful in the identification, isolation and/ or cloning of genes encoding insecticidally effective proteins or proteins required for viral activity, from HaSV or another virus (whether related or unrelated) carrying a similar gene or similar RNA sequence. They are also useful in screening for HaSV or other viruses in the field or in identifying HaSV or other viruses in insects, especially in order to identify related viruses capable of causing pathogenecity similar to HaSV. Any pair of oligonucleotide primers derived from eitiier RNA 1 or RNA 2 and located between ca 300 and 1500 bp apart can be used as primers. The following pairs of primer sequences exemplify particularly preferred embodiments of die present invention: Specifically for RNA 1: 1. HVR1B5' (described below) and die primer complementary to nucleotides 1192-1212 of Figure 1. 2. The primer corresponding to nucleotides 4084 and 4100 of Fig. 13 and die primer HVR13p described below Specifically for RNA 2: 1. The primer corresponding to nucleotides 459 to 476 of Fig. 2 and d e primer complementary to nucleotides 1653 to 1669 of Fig. 2 (tiiis would include die central variable domain) 2. R2cdha5 and die primer complementary to nucleotides 1156 to 1172 of Fig. 2 3. The primer corresponding to nucleotides 1178 to 1194 and d e primer complementary to nucleotides 2072 to 2091 (of Fig. 2). Otiier combinations giving shorter fragments are also possible. Further preferred primers include: j SUBSTITUTE SHEET 1 5 ' GGGGGGAATTCATTTAGGTGACACTATAGTTCTGCCTCCCCGGAC (called "HvRlSP5p" herein) 5' GGGGGGATCCTQGTATCCCAGGGGGGC (called "HvR13p" herein) 5' CCGGAAGCTTQTTTTTCTTTCTTTACCA (called "Hr2cdna5" herein) 5' GGGGGATCCGATGGTATCCCGAGGGACGC TCAGCAGGTGGCATAGG (called "HvR23p") herein AAATAATTTTGTTACTTTAGAAGGAGATATACATATGAGCGAGCGA GCACAC (called "HVPET65N" herein) AAATAATTTTGTTTAACCTTAAGAAGGAGATCTACATATGCTGGAGT GGCGTCAC (called "HVPET63N" herein) GGAGATCTACATATGGGAGATGCTGGAGTG (called "HVPET64N" herein) GTAGCGAACGTCGAGAA (called "HVRNA2F3" herein) GGGGGATCCTCAGTTGTCAGTGGCGGGGTAG (called "HVP65C" herein) GGGGATCCCTAATTGGCACGAGCGGCGC (called "HVP6C2" herein) AATTACATATGGCGGCCGCCGTTTCTGCC (called "HVP6MA" herein) AATTACATATGTTCGCGGCCGCCGTTTCT (called "HVP6MF" herein) The invention also relates to vectors encoding die nucleotide sequence described above and to host cells including die same. Preferably these vectors SUBSTITUTE SHEET - 12 - are capable of expression in animal, plant or bacterial cell or are capable of transferring the sequences of the present invention to the genome of other organisms such as plants. More preferably they are capable of expression in insect and crop plant cells. In a preferred aspect the invention relates to the vectors pDHVRl pDHVRlRZ, pDHVR2, pDHVR2RZ, pl7V71, pl7E71, pPH, pV71, pl7V64 P17E64, pP64, pV64, pBacHVRl, pBacHVRlRZ, P BacHUR2, P BacHVR2RZ PHSPR1, pHSPRlRZ, pHSPR2, pHSPR2RZ, pSRl(E3)A, pSRl(E3)B, PSR2A, pSR2B, pSX2P70, pSXR2P70, pSRP2B, pBHVRIB, P BHVR2B PT7T2P64, pSR2P70, pT7T2P65, pT7T2P70, pT7T2-P71, pBSKSE3, pBSR15 pBSR25p, pSR25, phr236P70, phr235P65, pGemP63N, pGemP64N P GemP65N, P P64N, pP65H, pTP6MA, pTP6MF, pTP17, pTP17deffiB, pP656 or p70G as described hereinafter. In a third aspect the invention relates to polypeptides or proteins encoded by HaSV and to homologues and analogues thereof. This aspect of the invention also relates to derivatives and variants of the pol^ptides and proteins of HaSV. Such derivatives and variants include substitutions and/or deletions of one or more amino acids, and amino and carboxy terminal fusions with other polypeptides or proteins. In a preferred aspect the invention relates to the proteins P7, P16, P17, P64, P70, P71, Plla, Pllb, P14 and P187 described herein and to homologues and analogues thereof, including fusion proteins particularly of P71 such as P70 described herein. In a most preferred aspect the i nvention relates to polypeptides or proteins from HaSV which have insecticidal activity themselves or provide target specificity for insecticidal agents. In particular the invention relates to polypeptides or fragments thereof w i th i nsect gut binding specificity, particularly to the variable domains thereof as herein described. In addition, homologues and analogues with said insecticidal activity of the polypeptides and proteins are also included within the scope of the invention. In addition the invention also relates to antibodies (such as monoclonal or polyclonal antibodies or chimeric antibodies including SUBSTITUTE SHEET j phage antibodies produced in bacteria) specific for said polypeptide and protein sequences. Such antibodies are useful in detecting HaSV and related viruses or the protein products tiiereof . In a fourth aspect the invention provides an infectious, recombinant insect virus including a vector, an expressible nucleic acid sequence comprising all of, or a portion of the HaSV genome, including an insecticidally effective portion of the genome and optionally, material derived from another insect virus species or isolate(s). Insect virus vectors suitable for the invention according to this aspect, include baculoviruses, entomopoxviruses and cytoplasmic polyhedrosis viruses. Most preferably, the insect virus vector is selected from the group comprising the baculovirus genera of nuclear polyhedrosis viruses (NPVs) and granulosis viruses (GVs). In this aspect of the invention the vector acts as a carrier for the HaSV genes encoding insectidical activity. The recombinant insect virus vector may be grown by either established procedures Shieh, (1989), Vlak (in press) or any other suitable procedure and the virus disseminated as needed. The insect virus vectors may be those described in copending International application No. PCT/AU92/00413. The nucleic acid sequence or sequences incorporated into the recombinant vector may be a cDNA, DNA or DNA sequence and may comprise the genome or portion thereof of a DNA or RNA of HaSV or another species. The term "material derived from another insect virus species or isolate" includes any nucleic acid sequence, or protein sequence or parts thereof which are useful in exerting an insecticidal effect when incorporated in the recombinant vector of the invention. Suitable nucleic acid sequences for incorporation into the recombinant vector include insecticidally effective agents such as a neurotoxin from the mite Pyemotes tritici (Tomalski, M.D. & Miller, L.K. Nature 352, 82-85 (1991) a toxin component of the venom of the North African scorpion Androcton s australia Maeda, S. et al. Virology 184-777-780 SUBSTITUTE SHEET (1991) Stewart, L.M.D. et al., Nature 352, 85-88 (1991), Conotoxins from the venom of Corns spp. (Olivera B.M. et al., Science 249, 257-263 (1990); Woodward S.R. et al., EMBO J. 9, 1015-1020 (1990); Olivera B.M. et al., Eur. J. Biochem. 202, 589-595 (1991). The exogenous nucleic acid sequence may be operably placed into the insect virus vector between a viral or cellular promoter and a polyadenylation signal. Upon infection of an insect cell, the vector virus will cause the production of either infectious virus genomic RNA or infectious encapsidated viral particles. The promoters may be constitutively expressed or inducible. These include tissue specific promoters, temperature sensitive promoters or promoters which are activated when the insect feeds on a metabolite in the plant that it is desired to protect. Recombinant insect virus vectors according to the present invention may include nucleic acid sequences comprising all or an infectious or insecticidally effective portion of genome the HaSV and optionally another insect virus species or isolate. In a particularly preferred embodiment of the present invention there is provided assembled capsids comprising one or more of the capsid proteins of the present invention, or derivatives or variants thereof as contemplated or described herein. These assembled virus capsids are useful as vectors for insecticidal agents. As such the assembled viral capsids may be used to administer insecticidal agents such as various nucleotide sequences with insecticidal activity or various toxins to an insect. Nucleotide sequences in the form of RNA or DNA which can be used include those of the HaSV genome or other insect viruses. Toxins which can be used advantageously include those which are active intracellularly and may also include neurotoxins with an appropriate transportation mechanism to reach the insect neurones. SUBSTITUTE SHEET The efficacy or insecticidal activity of infectious genomic RNA or viral particles produced by insect cells infected with insect vectors according to this aspect of die invention, may be enhanced as described below. Moreover the virus vector itself may include within a non- essential region(s), one or more nucleic acid sequences encoding substances that are deleterious to insects such as the insecticidally effective agents described above. Alternatively an extra genome component may be added to the HaSV genome either by insertion into one of die HaSV genes or by adding it to die ends of die genome. In a particularly preferred embodiment there is provided a recombinant baculovirus vector comprising HaSV or part diereof having insecticidal properties. Other modifications which may be made to the infectious recombinant insect virus according to die fourth aspect include: i) splitting the exogenous HaSV nucleic acid molecules comprising the genome and cloning die fragments into die insect vector so that tiiey cannot rejoin. One component, preferably the virus RNA replicase, could be expressed from a separately-transcribed fragment, the transcripts of which would not be replicated by the replicase they encode. The remainder of die genome (having insecticidal activity or encoding the capsid protein or a separate toxin m-RNA) could be encoded by, for example, a second separately-transcribed fragment, die transcripts of which are capable of being amplified by the replicase. Consequendy, whilst the transcripts from the second or otiier fragment would effect tiieir insecticidal activity upon the infected insect cell, they would not be able to infect anotiier insect ceU, (even if encapsidated) because the replicase or replicase-encoding transcripts would be absent; This modification would allow an inherent biological containment to be built into the insecticidal vectors, which, when used in conjunction witii SUBSTITUTE SHEET - 16 - the use of non-persistent DNA virus vectors such as those described in the above mentioned copending application, would allow a new level of environmental safety greatiy extending earlier approaches based on baculovirus vectors. ϋ) Manipulation of encapsidation signals or sequences essential for replicase binding or production of sub-genomic mRNA's including expression of exogeneous insect control factors as RNAs dependent on the virus for replication. This involves determination of RNA sequences and signals important for replication and encapsidation of virus RNAs, such as by analysis of replication of deletion mutants carrying reporter genes in appropriate cells, followed by studies on the transmission of the reporter gene to larvae by feeding of virus. These deletion mutants can be used to carry genes for insect control factors/toxins to larvae after replacing the reporter gene by a suitable toxin gene such as shown in Fig. 12; iii) using an insect promoter responsive to virus infection and, for example, placing copies of the viral replicase gene under the control of two promoters, one which is constitutive or expressed at early stages of vector infection, and the other being a cellular promoter turned on by the ensuing RNA viral infection. This system would then make more copies of the replicase mRNA available as the amount of its template increased. Such a promoter may be isolated using techniques analogous to enhancer trapping, that is, transforming insect cells with a suitable reporter gene and looking for induction of the reporter upon virus infection of a population of transformed cells. In a fifth aspect the invention relates to a method of controlling insect attack in plants by genetically manipulating plants to express HaSV or parts thereof which can confer insecticidal activity optionally in combination with other insecticidally effective agents. Such plants are referred to as transgenic plants. SUBSTITUTE SHEET The term "express" should be understood as referring to the process of transcribing the genome or portion thereof into RNA or, alternatively, the process of transcribing the genome or portion ώereof into RNA and then, in turn, translating the RNA into a protein or peptide. In a sixth aspect the invention relates to d e transgenic plants pense. as described above. Transgenic plants according to die invention may be prepared for example by introducing a DNA construct including a cDNA or DNA fragment encoding all or a desired infectious portion of HaSV, into die genome of a plant. The cDNA or DNA fragment may, preferably, be operably placed between a plant promoter and a polyadenylation signal. Promoters may cause constitutive or inducible eapression of die sequences under tiheir control. Furthermore tiiey may be specific to certain tissues, such as die leaves of a plant where insect attack occurs but not to otiier parts of die plant such as that used for food. The inducible promoters may be induced by stimuli such as disturbance of wind or insect movement on the plant's tissues, or may be specifically turned on by insect damage to plant tissues. Heat may also be a stimulus for promoter induction such as in spring where temperatures increase and likelihood of insect attack also increases. Otiier stimuli such as spraying by a chemical (for instances a harmless chemical) may induce die promoter. The cDNA or DNA fragment may encode all or a desired infectious portion of the wild-type, recombinant or otherwise mutated HaSV. For example, deletion mutants could be used which lack segments of the viral genome which are non- essential for replication or perhaps pathogenicity. The nucleotide sequences of die invention can be inserted into a plant genome by already established techniques, for example by an Agrobacterium transfer system or by electroporation. Plants which may be used in this aspect of the invention include plants of both economic and scientific interest. Such plants may be those in general which SUBSTITUTE SHEET need protection against the insect pests discussed herein and in particular include tomato, potato, corn, cotton, field pea and tobacco. To enhance die efficacy of infectious genomic RNA or viral particles expressed by transgenic plants according to the invention, the DNA construct introduced into d i e plants' genome may be engineered to include one or more exogenous nucleic acid sequences encoding substances that are deleterious to insects. Such substances include, for example, BacUlus tiiwingiensis δ-toxin, insect neurohormones, insecticidal compounds form wasp or scorpion venom or of heterologous origin, or factors designed to attack and kill infected cells in such a way so as to cause patiiogenesis in the infected tissue (for example, a ribozyme targeted against an essential cellular function). DNA constructs may also be provided which include: i) mechanisms for regulating pathogen expression (for example, mechanisms which restrict die expression of ribozymes to the insect cells) by tying for example, expression to abundant virus replication, production of minus-strand RNA or sub-genomic mRNA's; and/or ii) mechanisms similar to, or analogous to, those described in copending International patent application number PCT/AU92/00413 so as to achieve a limited-spread system (such as control of replication). Transgenic plants according to die present invention may also be capable of expressing all or an infectious or insecticidal portion of genomes from HaSV and one or more species or isolates of insect viruses. In a seventh aspect of the invention HaSV, or insecticidally effective parts ti i ereof, or the infectious recombinant virus vectors of the fourth aspect of the present invention may be applied direcdy to the plant to control insect attack. HaSV or the recombinant virus vectors may be produced either in whole or in ) SUBSTITUTE SHEET part in either whole insects or in culture cells of insects or in bacteria or in yeast or in some other expression system. HaSV or the recombinant virus forms may be applied in a crude form, semi purified or purified form optionally in admixture with agriculturally acceptable carrier to the crop in need of protection. HaSV may also be applied as a facilitator of infection where existing insect populations already infected with another agent, such as one or more other viruses whereby HaSV is able to act synergistically to bring about an insecticidal effect. Alternatively HaSV and anotiier agent such as one or more viruses may be applied together to plants to control insects feeding tiiereon. A deposit of HaSV No. 18.4 was made on August 5th 1992 at d e Australian Government Analytical Laboratories. The deposit was given accession No. N92/35575. EXAMPLE 1 TAXANOMIC, PHYSIOCHEMICAL AND BIOCHEMICAL CHARACTERISATION OF AN INSECT VIRUS: HaSV Materials and Methods A Animals and virus production. H. Armigara larvae were raised as described in Teakle R.E. and Jensen J.M. (1985) Heliothis punctiger in Singh P and Moore R.F. (eds) Handbook of Insect Rearing Vol 2., Elsevier, Amsterdam pp 313-322. Larvae were infected for virus production by feeding five day old larvae on lOmg pieces of diet to which 0.064 OD 2 6 0 units of HaSV had been applied. After 24 hours the larvae were then transferred to covered 12-well plates (BioScientific, Sydney, Australia) that contained sufficient diet and grown for eight days after which they were collected and frozen at -80 °C until further processed. Frozen larvae were weighed to lOOg, placed into 200ml of 50mM Tris buffer (pH 7.4), homogenized, and filtered through four layers of muslin. This homogenate was centrifuged in a Sorvall SS-34 rotor at 10,000 x g for 30 minutes whereupon the supernatant was j SUBSTITUTE SHEET transferred to fresh tubes and recentrifuged in Beckman SW-28 rotor at 100K xg for 3 hours. The resultant band was collected and repelleted in 50 mM pH 7.2 Tris buffer in a Beckman SW-28 tube by centrifugation at 100K xg for 3 hours. The pelleted virus was resuspended overnight in 1ml of buffer at 4 β C then layered onto a discontinuous CsCl gradient containing equal volumes of 60% and 30% CsCl (w/v) in a Beckman SW-41 tube and centrifuged at 12 h at 200 xg. The resultant pellet was suspended in lOOμl of buffer and frozen for further use. B Particle characterization. Staining with acridine orange was as described in Mayor H.D. and Hill N.O. (1961) Virology 14: ρ264. Buoyant density was estimated in CsCl gradients according to Scotti PX)., Longworth J.F., Plus N, Crozier G. and Reignanum C. (1981) Advances in Virus Research 26; 117-143. C Immunological procedure. Rabbit anti-sera to HaSV was produced by standard immunological procedures. Rabbit antisera to the Nudaurelia o virus in addition to the virus itself was provided by Don Hendry (Rhodes University, Grahamstown, South Africa). Rabbit antisera to the Nudaurelia b virus was supplied by die late Carl Reinganum (Plant Research Institute, Burnley, Vic, Australia). The immunological relationship to the Nudaurelia ω virus was determined by the standard reciprocal double diffusion technique. Immunoblotting was performed according to Towbin H., Staeheln T. and Gordon J. (1979) PNAS. Antibodies monospecific for the major 65 kDa capsid protein were prepared by incubating polyclonal antisera with sections of nitrocellulose blotted with the 65 kDa protein. After extensive washing in Tris buffered saline, the bound antibodies were eluted in 50mM citric buffer, pH 8.0 after a 5 minute incubation. D Protein characterization. Polyacrylamide gel electrophoresis in the presence of SDS followed die procedure of Laemmli UK 1970 Nature SUBSTITUTE SHEET 222 680-685 and was done witii 12.5% gels unless otherwise noted witii low and high molecular weight standards from BioRad. Staining was done witii a colloidal preparation of Coomassie Blue G-250 (Gradipore Ltd, Pyrmont, New South Wales, Australia). Determination of the M r of the smallest protein was done witii a 16% gel and standards of 3.4 kDa, 12.5 kDa and 21.5 kDa (Boehringer Mannheim). Glycosylation of the viral proteins was determined by a general glycan staining procedure with reagents supplied by Boehringer Mannheim; the positive control was fetuin. N-termini of proteins were sequenced using procedures described by Matsudairia (1989) Purification of Proteins and Peptides by SDS-PAGE in A Practical Guide to Protein and Peptide Purification for Microsequencing ed Matsudaira P.T. Academic Press, San Diego pp 52-72 on an Applied Biosystems 477A gas phase sequencer. Nucleic acid characterization. RNA was removed from capsids by twice vortexing a virus suspension with equal volumes of neutralized phenol tiien with phenol /chloroform (50:50). RNA was then precipitated from the aqueous phase in die presence of 300 mM sodium acetate and 2.5 volumes of etiianol. Digestions of the HaSV nucleic acid with RNAse A and DNAse I (Boehringer Mannheim) were done with pBSSK(-) phagemid ssDNA and dsDNA (Stratagene) and RNA controls (BRL). Denaturing agarose gel electrophoresis in the presence of formaldehyde was performed according to Sambrook et al (1989). The state of polyandenylation of the viral RNA was determined by two methods. The first method was to compare the binding of identical amounts (20 μg) of viral RNA and poly(A)-selected RNA from Helicoverpa virescens to a 1ml slurry of 5mg of oligo-d(T) cellulose (Pharmacia) in a binding buffer consisting of 20 mM Tris pH 7.8, 500 mM NaCl, 1 mM EDTA and 0.04% SDS. The second metiiod was to observe specific priming of viral RNA and viral RNA polyadenylated with poly(A) polymerase (Pharmacia) with d(T) j gA/C/G primers in RNA sequencing reactions using reverse transcriptase (US Biochemical) and a protocol provided ( SUBSTITUTE SHEET by the supplier. The 5' cap structure of the genomic RNA and HaSV was determined by observing the ability of polynucleotide kinase to phosphorylate viral RNA with and without preincubation with tobacco acid pyrophosphatase and alkaline phosphatase (Promega) under conditions described by the supplier. In vitm translation of HaSV RNA. In vitro translation of HaSV RNA was performed with lysates of both rabbit reticulocytes and wheat germ (Promega) as directed by the supplier. Reactions were conducted in 10 μl volumes with 1.0 μg of RNA in the presence of five u Ci 35 S- ethionine. The labelled proteins were resolved on 10% and 14% SDS-PAGE gels as described above then visualised by autoradiography of the dried gels. The two viral RNAs were separated by a "freeze and squeeze" method after resolution on nondenaturing low-melting-point agarose gels in TAE (Sambrook, et al. 1989). Briefly, agarose slices containing the RNA were melted at 65 • C in a volume of TAE buffer equal to six times the agarose volume. The solution was allowed to gel on ice before freezing at -80 β C for 30 minutes. The frozen solution was thawed on ice then centrifuged at 14,500xg for 10 minutes after which the supernatant was withdrawn and precipitated by the addition of etiianol. Bioassay of virus-induced pathogensis Known amounts of virus isolate, as shown in Figure 4, were fed to larvae at the growth stages indicated by admixture to stadnard diet. At die time points shown, the larvae were weighed and die mean and SD calculated. Growth of infected larvae was compared to tiiose of uninfected control populations from the same hatching batch in every experiment. SUBSTITUTE SHEET Results i) Characteristics and taxonomy of HaSV The virus particles are isometric and are approximately 36 - 38 nm in diameter. They are composed of two major capsid proteins, of 65 kDa and 6kD is size. The virions contain two single-stranded ( + ) RNA species of 5.3 kb and 2.4 kb lengdi. The virus bears a similarity in these respects to the Nudaurelia ω virus, which has been tentatively regarded as a member of die Tetraviridae; tiiese two viruses differ however, in die above respects from other viruses in this group and are likely to form a new virus family, sharing chiefly their capsid structure (T=4) with d e Tetraviridae. ii) Particle characterization and serology. The buoyant density of HaSV was calculated to be 1.296g/ml in CsCl at pH 7.2. The A 2 60 A 280 ratio of HaSV ^^ particles was 1.22 indicating a nucleic acid content of approximately 7% (Gibbs and Harrison, (1976) Plant Virology: The Principles London: Edward Arnold. Reciprocal immuno-double diffusion comparisons between HaSV and die Nudaurelia ω virus showed no serological relationship. The more sensitive technique of immunoblotting also showed a complete lack of any antigenic relationship. In addition, HaSV did not react with antisera to the Nudaurelia β virus in a immuno-diffusion test or when immunoblotted. However, no Nudaurelia β virus was available as a positive control in these latter two immunological experiments. When HaSV was stained witii acridine orange then irradiated with 310nm UV light, the particles fluoresced red which indicated a single stranded genome. iii) Protein characterization. Examination of d e capsid proteins of HaSV with polyacrylamide gel electrophoresis in the presence of SDS showed variable results depending on die quantity of protein present. At low protein loadings, two proteins in major abundance were evident tiiat had M r 's of 65,000 and 6,000 along witii a protein in minor abundance witii M r of 72,000 (data not shown). When more protein was present on the gels, however, at least 12 more distinct bands with M r 's SUBSTITUTE SHEET ranging between 15,000 and 62,000 became evident. Probing the resolved and blotted proteins with antibodies monospecific for the major 65 kDa capsid protein showed all but two of the proteins shared common antigens with die major 65 kDa protein. The major 6 kDa capsid protein and a minor band migrating at M r = 16,000 failed to react with botii die monospecific antibodies and untreated antisera. The capsid proteins were shown to be non-glycosylated as they failed to react with a hydrazine analog after oxidation with periodic acid. The N-terminus of the 65 kDa protein appeared to be blocked in some manner as two efforts to conduct an Edman degradation failed. After the second attempt, the sample was treated with n-chlorosuccinimide and shown to be in a quantity normally adequate for sequencing. The N-terminus of the 6 kDa protein, however, was not blocked as an unambiguous 16-residue sequence was readily obtained. The sequence of the N-terminus of the 6 kDa capsid protein and those of a cyanogen bromide cleaved fragment of the 65 kDa protein are as follows: 6 kDa protein: PheAlaAlaAlaValSerAlaPheAlaAlaAsnMetLeuSerSerValLeuLysSer 65 kDa protein: ProThrl^uValAspGlnGlyPheTrpUeGlyGlyGlnTyrAlaLeuThrProThrSer Detailed sequence analysis of the RNA genome carried out in Example 3 showed tiiat RNA 1 encodes a protein of molecular weight 186,980 hereinafter referred to as PI 87 and RNA 2 encodes proteins with molecular weight 16, 522 (called P17) and 70,670 (called P71). P71 is processed into two proteins of molecular weight 63,378 (called P64) and 7,309 (called P7). iv) Nucleic acid characterization The extracted nucleic acid from HaSV was readily hydrolysed by RNAse A but not by DNAse I. Denaturing agarose gel electrophoresis of the extracted RNA SUBSTITUTE SHEET genome of HaSV indicated two strands tiiat migrated at 5.5 kb and 2.4 kb. The RNA strands were shown not to have extensive regions of polyadenylation as only 24% of the viral RNA bound to die oligo-d(T) cellulose matrix as opposed to 82% of poly(A)-selected RNA. Further evidence for the non- polyadenylation of die viral genome was provided by die observation that the oligo primer, d(T) j gG, gave a clear sequencing ladder using reverse transcriptase only after in vitro polyadenylation of die viral strands with poly(A)-polymerase. The demonstration that the strands could be modified with poly(A)-polymerase also showed die lack of any 3' modification. The 5' termini of the viral strands were shown to be capped, most likely with m 7 G(5')ppp(5')G, as they could not be labelled with polynucleotide kinase unless pretreated with tobacco acid pyrophosphatase and alkaline phosphatase. v) In vitro translation. In vitro translation of the viral RNA yielded different results in the two translation systems used (data not shown). The 5.5 kb RNA translated very poorly in the reticulocyte system whereas it produced in the wheatgerm system more than 20 proteins ranging in size from M r = 195,000 to M r = 12,000. The 2.4 kb viral RNA strand yielded a major protein with an M r = 24,000 in both systems in addition to a minor protein at M r =70 kDa. A time course of the translation reaction with the 5.5 kb RNA strand showed all labelled proteins were produced at similar rates indicating tiiat the smaller products did not arise through processing of the larger ones. However when a time course experiment was done with translation of the smaller 2.4 kb RNA strand, the 24 kDa protein appeared before d e 70 kDa protein. vi) Presence of another form of HaSV Frequently, during purification of HaSV virions, a minor band appeared in varying amounts on the CsCl gradient that had a buoyant density of 1.3 g/ml. On four occasions, when particles from this minor band were used to infect H. SUBSTITUTE SHEET armigera larvae that were then processed as before for purification of HaSV virions, the HaSV band with a density of 1.296g/ml was again recovered in vast excess to a varying minor amount of the more dense band. No virions of either type were recovered from uninfected control larvae. Proteins extracted from the more dense particles appeared identical to tiiose from the less dense particles when examined by SDS-PAGE and immunoblotting with antibodies specific for the 65 kDa capsid protein of HaSV. Extraction and examination of the RNA genome with denaturing agarose gel electrophoresis also showed he same 5.5 and 2.4 kb bands. When particles from the more dense band were examined by electron microscopy as before, they appeared to have a larger diameter 45nm but otherwise highly similar to the 38nm particles. The molar ratio of the two RNA strands was determined by quantitative densitometry of fluorograms of the resolved strands. The ratio derived from an average of four measurements of various loadings on denaturing gels proved to be 1.7:1 (5.5 kb strand: 2.4 kb strand) which is somewhat lower than the expected ratio of 2.3:1 for equimolar amounts of each strand. The genome of HaSV has major differences that make it distinct from tiiose of the nodaviruses, die only other group of bipartite small RNA viruses pathogenic to animals. Although HaSV shares the characteristic of a bipartite genome with the only animal viruses having such a divided genome, die nodaviridae, it differs in virtually every other aspect from this group. Both segments of its genome are considerably larger than the corresponding nodaviral RNAs (Hendry DA, (1991) Nodaviridae of Invertebrates, in (ed. E. Kurstak) Viruses of Invertebrates. Marcel Dekker, New York, pp. 227-276). However, the division of genetic labour is similar with the larger component carrying the replicase gene and die smaller one encoding die capsid proteins. Direct comparison of the sequences shows little homology between these viruses, at either RNA or protein level. The Nodaviruses, have the already mentioned unusual 3 * blockage (probably a protein), whereas the HaSV RNAs terminate in a distinctive secondary structure resembling a tRNA. SUBSTITUTE SHEET vii) Bioassays of virus isolates on larvae The original constructs made to express the capsid proteins (precursor and processed forms) in E. coli for bioassay started at die first AUG (nts 284 to 286). Production of full-length, immuno-reactive protein from these was due to tiiese clones being the 5C sequence version with the extra C residue. Bioassays of these proteins have been difficult due to problems with obtaining suitable Heliothis larvae for the tests. EXAMPLE 2 OTHER VIRUS ISOLATES Materials and Methods A Virus isolation Apparently infected (viz diseased) larvae of Helicoverpa spwere collected in February 1993 at Mullaley (NSW), Narrabri (NSW) and Toowoomba (OLD) (Australia). Referring to Fig. 10 the samples in wells 2A-2D were from parasitised H. armigera larvae collected from sorghum at Mullaley, the sample in 6C was collected from sunflower at Toowoomba; the sample in 7D was collected from cotton at die Narrabri Research Station. The latter two larvae may have been either H. armigera or H. punctigera, which are both easily infected with ΗaSV. B Virus RNA Extraction Larvae collected were ground up and RNA extracted. RNA extraction and purification were as per Example 1. C Dot-Blot Northern Hybridization Extracts of viral RNA was analysed by Northern dot-blot hybridisation using a probe made from cloned HaSV sequences derived from 3'-teπninal 1000 units of RNA 1 and RNA 2 by random priming in a Boehringer Mannheim kit according to die supplier's instructions were employed. RNA extracts were transferred to Zeta-Probe (BioRad) for probing. Hybridization under high I SUBSTITUTE SHEET stringency washing conditions were as specified by BioRad. Hybridizations were carried out in the following solution: 1 mM EDTA, 500 mM HaH^O pH 7.2, 7% SDS, at 65 °C in a rotating Hybaid hybridization chamber. After completion of hybridization and removal of die solution containing the probe, the filters were washed twice in 1 mM EDTA, 40 mM HaH 2 P0 4 pH 7.2, 5% SDS, at 65 β C (1 h each), followed by 2 washes in 1 mM EDTA, 40 mM HaH^O,,, pH 7.2 1% SDS, at 65 °C (1 h each), before autoradiography. RESULTS Referring to Fig. 10, samples 9A, 9B, 10A, 10B and 10C contain HaSV infected positive control lab-raised larvae; 9C-H contain healthy (HaSV-free) negative control lab-raised larvae; All other wells (beginning 1-8) contain extract from field-collected larvae. Numbers 2A-D, 6C and 7D gave positive signals indicating that these isolates are either the same as HaSV or derivatives or variants thereof. Election microscopy employing (-) staining confirmed tiiat die samples which gave positive signals contained abundant icosohedral virus particles of approximately 36mm in size. The presence of HaSV in larvae which had tested positive in the Northern hybridization dot-blot was confirmed by Western blotting of crude extracts from such infected larvae, using the polyclonal antibody to die HaSV capsid protein. For routine screening of such extracts in order to identify further isolates of HaSV or to confirm the presence of the virus, use of a monoclonal antibody or its equivalent is preferable, in order to achieve (i) higher sensitivity of detection and (ii) greater specificity of detection. SUBSTITUTE SHEET EXAMPLE 3 IDENTIFICATION, ISOLATION AND CHARACTERISATION OF INSECT VIRUS GENES Materials and Methods A Animals and virus production. H. armigera larvae were raised as described in Example 1. B Protein characterization Was conducted as described in Example 1. C Nucleic acid characterization Was conducted as in Example 1. D Fractionation of virus RNA The two viral RNAs were separated by a "freeze and squeeze" method after resolution on nondenaturing low melting point agarose gels in TAE (Sambrook, et al, 1989). Briefly, agarose slices containing the RNA were melted at 65 ° C in a volume of TAE buffer equal to six times the agarose volume. The solution was allowed to gel on ice before freezing it at -80 ° C for 30 minutes. The frozen solution was thawed on ice tiien centrifuged at 14,500g for 10 minutes after which the supernatent was withdrawn and precipitated by the addition of ethanol. E In vitro translation of HaSV RNA Was as in Example 1. F cDNA synthesis and cloning of virus genome The virus RNAs were reverse transcribed into cDNA using the Superscript RTase (a modified form of the Moloney murine leukaemia virus (MMLV) RTase, produced by Life Technologies Inc). Oligo(dT) was used as a primer on RNA which had been polyadenylated in vitro After size selection of DNA fragments over 1 kbp in length, the cDNA was then blunt-end ligated using T4 SUBSTITUTE SHEET DNA hgase (Boehringer Mannheim or Promega, under conditions described by the suppliers) into vector pBSSK(-) (Stratagene) which had been cut with EcoRV and dephosphorylated with calf intestinal alkaline phosphatase (Boehringer Mannheim). E.coli strain JM109 or JPA101 were electroporated with the ligation mixture and white colonies selected on colour-indicator plates Sambrook eLal, 1989. For some clones of RNA2, cDNA was synthesised using the RTase of AMV (Promega) and a specific primer complementary to nucleotide sequence 2285 - 2301 of RNA 2. The same buffer and conditions were used for the Superscript RTase (above). The AMV RTase was found not to make cDNA form a primer annealing to the terminal 18 nucleotide sequence (see below), nor to be able to reach the 5'-end of the RNA with the primer here described. G Sequencing of DNA and RNA The cDNA clones were separated as single-stranded or double-stranded DNA, using the deaza-dGTP and deaza-dlTP nucleotide analogues (Pharmacia) in the deaza T7 sequencing kit as recommended by this supplier. Synthetic oligonucleotides were used as primers. The 5' terminal sequences of the two RNAs were determined using reverse transcriptase to sequence the RNA template directly, from specific ohgonucleotide primers located about 200 nucleotides downstream from the termini. Such RNA sequencing was performed using the reverse transcriptase sequencing kit from Promega, under d i e conditions described by the manufacturer. The sequence of the 20 or so nucleotides at the 5' terminus of each RNA was checked using direct RNase digestion of 5'-labelled RNA under conditions designed to confer sequence-specificity. Direct RNA sequence using RNases was performed with the RNase sequencing kit from US Biochemicals, following the protocols provided by the manufacturer. This also confirmed that the sequence of the most abundant RNA is consistent with that of the RNA analysed using the specific primer and RTase. j SUBSTITUTE SHEET All transcription of plasmids linearized as described were performed as recommended by the suppliers of SP6 RNA polymerase, in the presence of ImM cap analogue, 0.2mM GTP, and 0.5mM of the other NTPs. H Subcloning and expression PCR amplification The polymerase chain reaction (PCR) was used to obtain sequences covering virus genes in a form suitable for cloning into expression vectors. The reaction was performed with Taq DNA polymerase (Promega) as described by the supplier, in a rapid cycling thermal sequencer manufactured by Corbett Research (Sydney, Australia). A typical reaction involved 1 cycle of 1 min at 90 °C, 25 cycles of 95 β C (10 sec), 50 °C (20 sec), 72 °C (1.5 min), followed by one cycle of 72 β C for 5 min. Templates were generally cDNA or cDNA clones derived from HaSV RNAs, made as described below. Primers were as described below for the relevant constructs. Upon termination of the PCR reaction, the product's ends were made blunt by treatment with E.coli DNA polymerase I (Klenow fragment) at ambient temperature for 15 minutes. After heating at 65 ° C for 10 minutes, the reaction was cooled on ice and the reaction mix made ImM in ATP. The product tiien 5'-phosphorylated using 5 units of T4 polynucleotide kinase at 37 ° C for 30 minutes. After heating at 65 ° C for 10 minutes, the product was run on a 1% low-melting agarose gel and purified as described for RNA in section E above. ligations: Vectors and restriction fragments cut with the enzymes described were run on 1% low-melting-point agarose gels and excised as slices. These slices were then melted at 65 ° C for 5 minutes, before cooling to 37 ° C. Fragment and vectors were then ligated in lOul total volume at 14 ° C overnight using T4DNA ligase (BRL, Boehringer Mannheim or Promega), in the buffers supplied by the manufacturers. SUBSTITUTE " SHEET ea rcsHon ; Expression plasmids containing viral genes (e.g. for the capsid protein) were transformed into E. coli strain BL21 (DE3) or HMS174 (DE3) (supplied by Novagen). After growth as specified by die supplier, protein expression was induced by the addition of isopropyl β-D-thiogalactopyranoside (IPTG), at 0.4 nM to the growing culture for a period of 3h. Expressed proteins were analysed by SDS-polyacrylamide gel electrophoresis of bacterial extracts (Laemmli, 1970). Results i) Mapping cDNA clones of HaSV The template for cDNA synthesis was virus RNA which had been polyadenylated in vitro. Oligo(dT) was used as a primer for the Superscript reverse transcriptase (RTase; a modified form of the Moloney murine leukaemia virus (MMLV) RTase, produced by Life Technologies Inc). The cDNA was cloned into vector pBSSK(-) as described earlier. The larger clones were selected for further analysis by restriction mapping and Northern hybridization. All the probes tested hybridized either to RNA 1 or to RNA 2, suggesting that there are no regions of extensive sequence homology between the two RNA's. Furthermore, screening of a number of other clones excluded the theoretical possibility that either RNA band may actually contain more than one species. ii) RNA 1 clones Three large RNA1 clones (B11U, BllO and B35) obtained for the first round of clones were further analysed by restriction mapping and shown to form an overlap spanning over 3 kbp (this was later confirmed by sequencing). The second round of cloning then yielded E3 of 5.3 kbp, representing 99.7% of RNA 1. A complete restriction map of clone E3 showed it to align with that previously determined for three overlapping clones. On the basis of this alignment, the 5' end of the insert in B11U was placed about 300 nucleotides downstream from die 5' end of the RNA SUBSTITUTE SHEET Once clones covering a contiguous block had been identified, die orientation 3relative to the RNA was determined. iii) RNA 2 clones Three significant cDNA clones were isolated for RNA 2 (Fig. 2). One, hr236, contains about 88% of RNA 2 (2470 bp total length), and runs from the 3' end to 240 bp from the 5' end. The other clones, hr247 and hr 249 are 3' coterminal subgenomic fragments of 1520 bp and 760 bp, respectively. Orientation of clone hr236 was determined by strand specific hybridization. While a much stronger signal was seen with a probe for one orientation, the probe specific for the other orientation also yielded a signal, indicating that there are extensive regions of reverse complementarity within the positive strand sequence. Such sequences are likely to form extensive short and long- range secondary structure. The clones contain the 3' sequence of HaSV RNA 2 as they all have the same 3' sequence adjacent to the poly (A) stretch added in vitro before cDNA priming. The remaining 5' sequence of RNA 2 has been obtained by direct RNA sequencing using two reverse transcriptases as described above. iv) Sequencing of virus genome The clones mapped in section (i) were selected for further analysis by sequencing. The cDNA clones were completely sequenced as single-stranded DNA in both orientations, using the deaza-dGTP and deaza-dlTP nucleotide analogues (Pharmacia) and synthetic oligonucleotides as primers. v) Sequence of genome component 1 (see Figure 1) The 5310 nucleotides of RNA 1 encode a protein of molecular weight 187,000 which is regarded as the RNA-dependent RNA polymerase (replicase) in view of its amino acid sequence similarity in certain limited regions to replicases of SUBSTITUTE SHEET other RNA viruses. The apparent molecular weight of this protein upon in vitro translation of virus RNA and SDS-PAGE is 195,000. Sequence analysis of RNA 1 was concentrated on clone E3 which extends from the 3' end of RNA 1 to 18 nucleotides form the 5' end (Figure 1). The complete sequence has been confirmed by sequencing in both directions. An ORF of 1750 amino acids and spanning virtually the complete RNA (5310 nucleotides in length) has been detected. This ORF begins with the first AUG on the sequence at position 34 and terminates at nucleotide 5290 and is thought to encode the RNA-dependent RNA polymerase (replicase)(referred to as P187 in Fig. 1) required for virus replication, since it contains the Gly- Asp-Asp conserved triplet and surrounding sequences identified in these enzymes, which are usually large (over 100 kDa), in addition to further homology with the polymerase encoded by tobacco mosaic virus and other plus-stranded RNA viruses. Referring to Fig. 1 the sequence is presented as the upper strand of the cDNA sequence. This strand is therefore in the same sense as the viral (positive- sense) RNA The sequence of the protein encoded by the major open reading frame, encoding the putative RNA-dependent RNA replicase, is shown, as are those of the small open reading frames at the 3' end, corresponding to the proteins Plla, Pllb and P14. Clone E3 was inserted downstream of the SP6 promoter for in vitro transcription. As mentioned above, the transcript of this clone can be translated in the wheat germ system to yield the 195 kDa protein observed upon translation of fractionated RNA 1 from the virus. The latter yields more lower molecular weight products, presumably due to being contaminated with nicked and degraded RNA. The products derived from the in vitro transcript can therefore be regarded as defining the coding capacity of the complete RNA 1 of HaSV. SUBSTITUTE SHEET vi) Sequence of genome component 2 (see Figure 2) The 2470 nucleotides encode a protein of molecular weight 71,000 which contains the peptide sequences corresponding to tiiose determined from the two virus capsid proteins. This protein is therefore the precursor of these capsid proteins. The protein is a major product of in vitro translation of this RNA obtained either from virus particles or by in vitro transcription of a full- length cDNA clone; in addition, another major translation product of apparent molecular weight 24,000 is obtained. This protein is derived from a molecular weight 17,000 reading frame overlappling the slab of the capsid protein gene. Clones hr236 and hr247 were completely sequenced as the first step in RNA 2 sequencing. These sequences were then extensively compared to that obtained by direct RNA sequencing using AMV reverse transcriptase. Comparison of the cloned sequence with that by direct RNA sequencing showed both clones lacked 50 nucleotide present in the RNA (at around nucleotide 1500). The sequence of this stretch was obtained by direct RNA sequencing using the AMV RTase. The MMLV "Superscript" RTase, which was used to make all the cDNA clones, was found to simply by-pass this region in sequencing reactions. These 50 nucleotides contain a very stable GC-rich hairpin flanked by a 6 bp direct repeat, and the MMLV RTase skips from the first repeat to the second. The sequence of RNA 2 was then completed using plasmids pSR2A and pSR2P70 constructed as described below. The plasmids contain a segment of cDNA derived for the AMV RTase, as well as the sequence corresponding to the 5' 240 nucleotides of RNA 2 which are not present on phr236 (Fig. 2). The sequence of RNA in Fig. 2 is presented as the upper strand of the cDNA sequence. This strand is therefore in the same sense as the viral (positive- sense) RNA. The sequences of the proteins encoded by the major open reading frames, encoding the capsid protein precursor P71, and PI 7. SUBSTITUTE SHEET The sequence of RNA 2 encodes a major ORF running from a methionine initiation codon at nucleotides 366 to 368 to a termination codon at nucleotides 2307 to 2309. This protein encoded by this ORF has a theoretical molecular weight of 71,000. This initiation codon is in a good context (AGGatgG), suggesting that it will be well recognized by scanning ribosomes. The size of the product is close to that of the residual putative precursor protein identified in purified virus, and to die size of the in vitro translation product obtained from RNA 2. The approach adopted to identify the gene encoding die capsid protein was to obtain amino acid sequence information from the two abundant capsid proteins and tiien locate these on the protein encoded by die sequence of the virus RNA's. CNBr cleaved products of the capsid protein were therefore sequenced. These fragments gave a clear and unambiguous sequence shown in Example 1. These sequences determined were tiien located on die large ORF of RNA 2. (Figure 2) In the case of the small capsid protein, the clear and unambiguous sequence, obtained is located near the carboxy terminus of the major ORF on RNA 2. Starting at the point corresponding to the amino-terminal residue of the sequence determined for the 6 kDa protein, and continuing to the carboxy- teπninus of the complete reading frame, the protein encoded by the sequence 7.2 kDa and has a hydrophobic N-terminal region and an arginine rich (basic) C-terminal region. It is an extremely basic protein with a pi of 12.6. The two abundant capsid proteins are derived from a single precursor, which is processed at a specific site. This is presumably immediately amino-terminal to the sequence FAAAVS.... RNA 2 appears to be a bicistronic mRNA (see Figs. 2 and 5). The first methionine codon is encoded on the sequence of RNA at nucleotides 283 to 285. This ATG is in a poor context (TTTatgA), making it a weaker initiation SUBSTITUTE SHEET codon. It initiates a reading frame of 157 amino acids, encoding a protein of molecular weight 17,000. (The second AUG [nts 366 to 368] initiates the 71 kDa precursor of the capsid protein). Since the first AUG is in a poor context, abundant expression of the capsid precursor would be expected. In fact, in vitro translation of a full length RNA 2 transcribed from a reconstructed cDNA clone yields two major protein products of relative mobility 71,000 and 24,000, similar to those already observed upon translation of viral RNA 2. The protein of Mr 24,000 appears to correspond to die 157 amino acid protein, despite the significant anomaly in apparent size. The 24,000 Mr product was also observed upon translation of an in vitro transcript covering only nucleotides 220 to 1200 of RNA 2. This region contains no open reading frame other than those already mentioned and cannot encode a protein longer than 157 amino acids. The protein of Mr 24,000 seen upon in vitro translation appears to correspond to P17, with the anomaly in apparent size probably being due to die high content of proline (P), glutamate (E), serine (S) and ti reonine (T). These amino acids cause the protein run more slowly on a gel thereby giving it an apparent size of Mr 24,000. The Mr 24,000 protein (hereinafter referred to as P17) may have a function in modifying or manipulating the growth characteristics or cell cycle of HaSV- infected cells. Although a protein of 16kDa (identified in Example 1) is found in small amounts in the capsid, it does not react with antiserum against the virus particles this is unlikely to correspond to PI 7, since a preparation of the latter proteins migrates with a molecular weight of 24,000 on SDS gels. Sequence analysis of the Region from nucleotide 500 to 600 of RNA 2 showed tiiat it has the sequence shown in Fig. 2, as do die plasmids pSRZA, pSR2P70, pSR2B and pSXR2P70. However, plasmids pT7T72P65 and ρT7T2P70 have an extra C residue at nucleotide 570. The RNA sequence from which they are derived from is shown in Fig. 2 (the "5C" version). In this sequence the first SUBSTITUTE SHEET ATG (nucleotides 283 to 285) is in the same reading frame as most of the capsid protein gene. The resultant fusion protein is called "P70" and its carboxyterminal-truncated version (a variant of the native P64) is "P65". In view of these clones it was considered important to resolve whether any virus RNA carrying the extra C residue was present in the viral RNA population first isolated for investigation. Direct sequencing of the virus RNA using reverse transcriptase confirmed that the 4C version lacking the extra residue was the abundant form of the RNA. In order to exclude die possibility of a small amount of the RNA having the extra residue, a sensitive PCR assay was designed. This showed tiiat the extra C residue was not present on any RNA in the viral population, and had been introduced into some clones as a PCR artefact. These clones were however retained and used in bacterial expression experiments (below) because of the high level expression obtained of die P65 and P70 fusion proteins. vii) Comparison with the sequence of the Nudaurelia ω capsid gene The sequence of most of die RNA2 of the Nudaurelia ω virus has recently been published by Agrawal D.K. and Johnson J.E. (Virology 190 806-814, 1992). From the published sequence it has been determined tiiat this sequence shows 63% homology to that of HaSV RNA2 at die nucleotide level and 66% at d e overall amino acid level. A detailed comparison of the capsid proteins of these two viruses shows the amino-terminal 45 residues to be variable, the next 220 residues to be highly conserved, the next 180 residues to be variable and d e c-terminal 200 residues covering the small protein P7 to be highly conserved. A more detailed comparison is discussed below. The published report did not find a complete reading frame corresponding to die 157 amino acid protein (P17) gene reported above. The AUG is however present, as is a reading frame - starting upstream of the start of the capsid gene - showing considerable amino acid homology to PI 7 of HaSV. In vitro SUBSTITUTE SHEET translation of purified Nudaurelia ω virus RNA 2 and a re-examination of the nucleotide sequencing data for this RNA may help to resolve the question of whether the Nudaurelia ω virus also encodes a protein homologous to the HaSV P17. More interestingly, antisera against these two viruses, which are similar at a nucleotide sequence level, do not show any cross-reactivity. viii) Construction of full-length clones RNA 1 cDNA clone E3, described above contains all but the 5'-18 nucleotides of RNA 1 and included the complete ORF present on the sequence. The first full- length clone of RNA 1 is therefore based on E3. The 4.9 kbp Xbal-Clal fragment from clone E3 was recloned into pBSKS(-) (Stratagene) cut with Xbal and Clal, giving pBSKSE3. The full-length clone of RNA 1 was completed using PCR. The primer defining the 5' end of the RNA carried an EcoRI site, the promoter for the SP6 RNA polymerase and a sequence corresponding to the 5' 17 nucleotides of RNA 1, as shown in Figure 1. The sequence of this primer was: HvRlSP5p: 5'-GGGGGGAATTCATTTAGGTGACACTATAGTTCTGCCTCCCCGGAC (The G which initiates transcription is underlined) Using an ohgonucleotide complementary to nucleotides 1192 - 1212, a PCR product of 1240 bp was efficiendy made. The template was cDNA synthesised using the MMLV RTase and the same oligonucleotide complementary to nucleotides 1192 - 1212 was the primer. Upon termination of the PCR reaction, the product's ends were made blunt and then 5'-phophorylated as described below. The purified PCR fragment was then cleaved with restriction endonuclease Xbal and the 450 bp subfragment corresponding to the 5' end of SUBSTITUTE SHEET RNA 1 cloned into the plasmid pBSSK(-)(Stragene) cut with EcoRV and Xbal, to give pBSR15. To assemble the full-length of RNA 1, ρBSKSE3 (above) was cut with Xbal and Seal giving fragments of 1.2 kbp and 6.8 kbp. pBSR15 was cut with die same enzymes, giving fragments of 2 and 1.8 kbp. Ligation of the 6.8 kbp fragment for pBSKSE3 and the 1.8 kbp fragment for mpBSR15 yielded pSRl(E3)A Upon linearization at Clal and in vitro transcription with the SP6 RNA polymerase, and RNA corresponding to RNA 1, and terminating in a poly(A) stretch of about 50 nucleotides, is obtained. Since the natural RNA 1 does not have a poly (A) tail, an alternative plasmid was constructed which carries a BamHI restriction site immediately downstream of the 3'end of RNA 1. Again this terminal fragment was made using PCR as above. The sequence of the primer was as follows: HvR13p: 5'-GGGGGGATCCTQGTATCCCAGGGGCGC (the nucleotide complementary to that which was determined as the 3' one, based on its adjacency to the poly(A) stretch, is underlined; RNA terminating at the BamHI site will have the sequence GCGCCCCCUGGGAUACCaggauc). The template was clone E3 and an ohgonucleotide corresponding to nucleotides 4084 - 4100 was the other primer. The 1220 bp product was blunt- ended, kinased and gel-purified as described above, before cleavage with Hindlll. The resulting 420 bp subfragment corresponding to the 3' end of RNA 1 cloned into plasmid pSRl(E3)A cut with Clal, end-filled with Klenow and then cut with Hindlll. The resulting plasmid is pSRl(E3)B. Upon linearization at BamHI and in vitro transcription with the SP6 RNA polymerase, and RNA corresponding to RNA 1, and terminating as described immediately above is obtained. SUBSTITUTE SHEET ix) RNA 2 In constructing the full-length cDNA clone to enable in vitro transcription of this RNA hr236 described above was used as a basis. Two separate PCR products, one corresponding to die 5' portion of RNA 2, which is missing from this clone altogether, and another covering the region where clone hr236 lacks the hairpin-forming sequence described above, were required. The primer defining die 5' end of die RNA carried a Hindlll site and a sequence corresponding to die 5' 18 nucleotides of RNA 2, as shown in Figure 2. The sequence of this primer was: Hr2cdna5: 5'-CCGGAAGCTTGTTTTTCTTTCTTTACCA (The nucleotide underlined corresponds to tiiat identified as the first nucleotide of RNA 2.) Using an ohgonucleotide complementary to nucleotides 1653 - 1669, a PCR product of 1.67 kbp was made. The template was cDNA synthesised using the MMLV RTase and an ohgonucleotide complementary to the 18 nucleotides at die 3' end of RNA 2 as the primer. Upon termination of the PCR reaction, the product was blunt-ended, kinased and gel-purified as described above, before cleavage with Pstl. The resulting 1.3 kbp subfragment corresponding to the 5' half of RNA 2 was cloned into plasmid pBSSK(-) (Stragene) cut with EcoRV and Pstl, giving plasmid pBSR25p. In order to place this subfragment corresponding to the 5' half of RNA 2 downstream of the SP6 promoter for in vitro transcription, a 1.3 kbp Hindlll - BamHI fragment was excised from pBSR25p and ligated into Hindlll - BamHI cut pGEM-1 (Promega), giving plasmid ρSR25. The second PCR product, covering the region where clone hr236 lacks the hairpin-forming sequence described above, was synthesised using as primers oligonucleotides corresponding to nucleotide sequence 873 to 889 of RNA 2 and to the complement of nucleotide sequence 2290 - 2309. Upon termination of the PCR reaction, the product was blunt-ended, kinased and gel-purified as described above, before cleavage with Aatll. The resulting 1.1 kbp SUBSTITUTE SHEET subfragment covering the required region was cloned into plasmid phr236 cut witii Hindlll, end-filled with Klenow and cut witii Aatll, giving plasmid phr236P70. The two segments were joined covering the first 230 nucleotides of RNA 2 together. Plasmid phr236P70 was cut at the Sad site in the vector adjacent to the 5' end of die insert and this made blunt-ended using Klenow in the absence of dNTPs. After heat-inactivation of the Klenow, the plasmid was cut witii EcoRI, yielding fragments of 4.5 kbp and 380 bp. Plasmid pSR25 was cut with Nhel, blunt-ended by end-filling with Klenow and cut with EcoRI, yielding fragments of 2.8 kbp, 900 bp and 750 bp. The 4.5 kbp fragment of phr236P70 and die 900 bp fragment of pSR25 were ligated to give pSR2P70. This clone covers all of RNA 2 except for the 3' 169 nucleotides. To complete the full-length clone of RNA 2, it was necessary to insert a fragment covering the 3' end. As with RNA 1, two versions were made. One, called pSR2A, used die 3' end as present in phr236, togetiier with the poly(A) tail present in this version. The other pSR2B, used a PCR fragment carrying a BamHI site immediately downstream of die 3' nucleotide, as in pSRl(E3)B above. To construct ρSR2A, a 350 bp Notl-Clal fragment was excised from ρhr236 and cloned into pSR2P70 cut with the same endonucleases. Linearization at the unique Clal site allows in vitro transcription of the complete RNA 2 and a poly(A) tail of about 50 nucleotides in length. To make pSR2B, an appropriate PCR product was made using as primers an ohgonucleotide corresponding to nucleotide sequence 1178 to 1194 and to the 3' terminal 18 nucleotides of RNA 2. The latter primer carried a BamHII site attached, giving it the sequence: HvR23p: 5'-GGGGGATCCGATGGTATCCCGAGGGACGC The template used was a plasmid phr236. Upon termination of the PCR reaction, the product was blunt-ended, kinased and gel-purified as described ! SUBSTITUTE SHEET above, before cleavage with Notl. The resulting 400 bp subfragment covering the required region was cloned into plasmid pSR2P70 cut with Clal, end-filled with Klenow and cut with Notl, giving plasmid pSRP2B. Linearization at the unique BamHI site allows in vitro transcription of the complete RNA 2, terminating with the sequence ACCaggatc. x) Construction of pSXR2P70 This plasmid was made to determine where p24 starts. A 2.1 kbp Xhol- BamHI fragment was cut from clone pSR2P70 and ligated into the vector ρGEM-1 (Promega) which had been cut with Sail and BamHI. In vitro transcription of the resulting plasmid after linearization at the unique BamHI site yielded an RNA covering about 70 nucleotides upstream of the first ATG at nucleotides 283 to 286, plus a short sequence derived from the vector. In vitro translation of the RNA from ρSXR2P70 yielded both proteins (P70 + P24). xi) Description of virus-induced pathology The virus induces a rapid anti-feeding effect in Helicoverpa larvae as determined by experiments with larvae the results of which are shown in Fig. 3. Fig. 3 shows: A. neonate larvae (less than 24 h old) were fed die designated concentrations of isolated virus (in particles per ml [of diet] added to solid diet). They were weighed on following days and die mean of a statistically significant number (24) of larvae shown. Where necessary, mortality was recorded for the higher concentrations. The vertical axis shows the fold- increase in weight from the hatching weight of 0.1 mg per larvae. This scale therefore also corresponds to weight in units of 0.1 mg (ie 300 is equivalent to 30 mg). B. As for A, but the larvae were 5 days old at the start of the virus feeding. The vertical scale is in mg weight. No weight gain at all was detectable witii neonates which had been fed die doses of virus over 10 8 particles per ml (virus added to diet). In addition, SUBSTITUTE SHEET 100% mortality was evident after four days at die highest doses. Virus doses as low as 10 5 particles per ml (virus added to diet) still cause significant stunting. The five day old larvae showed a cessation of feeding after 48 hours and significant stunting at 4 dpi, but no mortality at comparable virus doses (Figure 3). Neonates are therefore very sensitive indeed to tiiis virus. Virus particles accumulate specifically in the midgut. This potent anti-feeding effect may be due to the capsid protein or another protein encoded by die virus, or to the effect of any combination of such proteins. xii) Expression of virus-encoded proteins in bacteria. The vectors The expression system used initially was derived from the pET-11 system (Novagen). Trimmed down versions of pET-llb and c were constructed and used to compare expression of the capsid proteins. However, due to difficulties experienced with this system substantial modification of the original vectors was carried out in order to achieve much higher yields. These results are described in xiii-b) below. The initial trimmed-down vectors discussed above were made as follows: pGEM-2 (Promega) which carries T7 promoter adjacent to a poly-linker sequence, but has no sequences corresponding to die lac operon, was cut at the unique Xbal (34) and Seal (1651) sites, giving fragments of 1.61 and 1.25 kbp. The plasmids pET-llb and c were cut with the same enzymes, giving fragments of 4.77 and 0.91 kbp. The 1.61 kbp fragment of pGEM-2, carrying the c- terminal portion of the ampicillin-resistance gene, the origin of rephcation and die T7 promoter, was then ligated to the 0.91 kbp fragment of the pET vector, which carries a sequence covering the Shine-Dalgarno sequence, the ATG (in a Ndel site), the terminator for the T7 polymerase and die N-terminal portion of the ampicillin-resistance gene. The resulting plasmids of approximately 2.53 kbp, called pT7T2-b and c, tiierefore carry a complete T7 transcription unit, which may be used as an expression system in a manner similar to the original pET-11 plasmids, but are repressor-neutral within the cell; they neither titrate SUBSTITUTE SHEET away repressor by carrying a binding site, nor do tiiey carry the gene producing die repressor. They were found to grow very well in E.coli strains JM109 and BL21 (DE3), and to be very efficient expression vectors. The repressor present in the cells was found to be sufficient to keep the genomic T7 polymerase gene uninduced and therefore the foreign gene unexpressed in the absence of IPTG. xϋi-a) Construction of plasmids for expression of capsid proteins In this section, all proteins expressed from segments of HaSV RNA 2 are referred to by die size of their gene, as defined in Fig. 4 and in section vi) of this example. The following plasmids were constructed by PCR, using the abovementioned full-length clone of RNA 2, plasmid pSR2A as the template, except where mentioned otherwise. Groups of plasmids expressed protein starting at each of the first three methionine initiation codons found on the sequence of HaSV RNA 2. For those proteins initiating at the first methionine initiation codon found on the sequence of HaSV RNA 2 (which initiates d e P17 gene; ohgonucleotide primer HVPET65N), an extra group of plasmids was made by PCR using as a template the version of the RNA 2 sequence carrying an extra C residue inserted at residue 570 (as depicted in Figure 2). Expression constructs initiating at the third methionine initiation codon found on the sequence of HaSV RNA 2 (which is located within die P17 gene; ohgonucleotide primer HVPET63N) were made by PCR using as a template only the verion of the RNA 2 sequence carrying an extra C residue inserted at residue 570. For these latter expression constructs, as well as those designed to initiate expression from the second methionine initiation codon found on die sequence of HaSV RNA 2 (which initiates the P71 gene; ohgonucleotide primer HVPET64N), two versions were constructed. One version terminated at a point corresponding to d e c-terminus of the processed (P64) form of the capsid protein and was made using SUBSTITUTE SHEET oligonucleotide primer HVP65C. The other version terminated at a point corresponding to the c-terminus of the precursor (P71) form of the capsid protein and was made using ohgonucleotide primer HVP6C2. The sequence encoding P64 (or the precursor, P71) was synthesised in two segments using PCR. The amino-terminal half of the gene was obtained using as primers oligonucleotides incorporating one of the three ATG possible initiation codons for the ORF, in addition to an ohgonucleotide with the sequence TCAGCAGGTGGCATAGG; complementary to nucleotides 1653 to 1669 of die sequence shown in Fig. 2. The forward primers were as follows: HVPET65N: AAATAATTTTGTTTACTTTAGAAGGAGATATACATATGAGCGAGCCTA GCACAC (the underlined sequence corresponds to nucleotides 283 to 296 of the sequence shown in Figure 2) HVPET63N AAATAATTTTGTTTAACCTTΛ GAAGGAGAT CTACATATGCTGGAGTG GCGTCAC (the underlined sequence corresponds to nucleotides 373 to 390 of the sequence shown in Figure 2; the Aflll (CTTAAG) and Bglll (AGATCT) sites introduced into the sequence by single nucleotide changes (shown in italics) in the ohgonucleotide are shown in bold). HVPET64N GGAGATCTACATATGGGAGATGCTGGAGTG (the underlined sequence corresponds to nucleotides 366 to 383 of die sequence shown in Figure 2; the Bglll site introduced into the sequence by a single nucleotide change in the oligonucleotide is shown in bold). The PCR products obtained from each combination of one of these primers with the abovementioned one were treated with the Klenow fragment of E.coli SUBSTITUTE SHEET DNA polymerase, and then with T4 polynucleotide kinase in the presence of 1 mM ATP, before purification by agarose gel electrophoresis as described above. Each product was then cleaved with Aatll to yield fragments of 0.95 and 0.4 kbp, and each resulting fragment of about .95 kbp cloned intro vector pGEM-2 (Promega) cut with Hindi and Aatll, giving plasmids pGEMP63N (in which the insert commenced with ohgonucleotide HVPET63N), pGEMP64N (in which the insert commenced with ohgonucleotide HVPET64N) and pGemP65N (in which the insert commenced with ohgonucleotide HVPET65N). The fragment covering portion of the HaSV capsid gene was then excised with enzymes Aatll and Xbal. Two versions of plasmid pGemP65N were made, using different templates as described above. pGemP65N was derived from the sequence of the viral RNA, as in plasmid pSF2A; plasmid pGemP65Nc was derived from the sequence carrying an extra C residue, as shown in Fig. 2 (see "5C version"). In parallel, the carboxy-terminal halves of the major capsid protein variant, whether terminating as for P64 or for P71, were also produced using PCR. An ohgonucleotide primer, HVRNA2F3, with the sequence GTAGCGAACGTCGAGAA (corresponding to nucleotides 873 to 889 of the sequence shown in Figure 2) was used in conjunction with each of the two primers following: HVP65C GGGGGATCCTCAGTTGTCAGTGGCGGGGTAG (the underlined sequence is complementary to nucleotides 2072 to 2091 of the sequence shown in Figure 2). HVP6C2 GGGGATCCCTAATTGGCACGAGCGGCGC (the underlined sequence is complementary to nucleotides 2290 to 2309 of the sequence shown in Figure 2). i SUBSTITUTE SHEET 660 - 48 - The PCR products obtained from each combination of one of these primers with the above mentioned one (HvRNA2F3) were treated with the Klenow fragment of tøHDNA polymerase, and then with T4 polynucleotide kinase m the presence of 1 mM ATP, before purification by agarose gel electrophores i s as described above. Each product was then cleaved with Aatll to yield fragments of 0.9 kbp (in the case of HVP65C) or 1.1 kbp (in the case of HVP6C2) and 0.4 kbp, and each resulting fragment of about .9 or 1.1 kbp cloned into plasmid phr236 cut with Hindlll, treated with Klenow and Aatll, giving plasmids phr236P65C and phr236P70 ( fcich has already been described above), respectively. The fragment covering the c-terminus of the capsid protein gene was then excised with enzymes Aatll and BamHI. To assemble plasmids for expression in suitable strains of E. coli, the excised Xbal-Aatll fragments of 0.95 kbp covering the amino-terminal half of the gene and the excised Aatll - BamHI fragments of 0.9 or 1.1 kbp covering the carboxy terminal half of the gene were simultaneously ligated into the vector pT7Ω cut with Xbal and BamHI. Initial transformation was of E. coli strain JM109. Recombinant plasmids carrying the correct insert were then transformed into strain BL21(DE3) for expression as described above. The plasmid obtained by ligating the aminoteπninal fragment commencing with ohgonucleotide primer HVPET63N to the c-terminal fragment ending at ohgonucleotide priemr HVP65C in the epxression vector P T7T2b was called pP65G. In the case of plasmid pP64N, containing an insert from HVPET64N to HV65C, the fragment covering the amino-terminal half of the ohgonucleotide was excised by Bglll and Seal from the plasmid P GemP64N and the fragment covering the remainder of the gene was excised with Seal and EcoRI from plasmid P T7T2-P65. These two fragments were then hgated simultaneously into pP65G which had been cut with Bglll sand EcoRI. SUBSTITUTE SHEET The resulting construct carrying the complete P71 precursor gene was called pT7T2-P71 and that carrying the P64 form of the gen was called pT7T2-P64. In the case of plasmids derived from ρGemP65N and pGemP65Nc, carrying inserts commencing as defined by primer HVPET65N, die expression plasmid derived from pGemP65N which is based on PCR products made using as the template the sequence of the viral RNA, as in plasmid pSR2A, was called ρTP17; a truncated form of this plasmid, which expresses P17, was made by cutting at the unique Bglll and BamHI sites, removing the intervening fragment (which corresponds to the c-terminal part of the insert) and religating the compatible cohesive ends, to give pTP17delBB. The expression plasmids derived from plasmid pGemP65Nc (which was derived from the sequence carrying an extra C residue, were called ρT7T2-P65 (carrying an insert terminating at the primer HVP65C) and pT7T2-P70 (carrying an insert terminating at the primer HVP6C2). Expression of P6 Two forms of this protein, which arises through processing of the large capsid protein variant precursor p70 and therefore lacks its own initiation codon, were made. One form (protein MA) replaced the phenylalanine at the start of this protein with methionine, giving it the amino-terminal sequence MAA..; the other carries an additional methionine residue, giving it the amino-terminal sequence MFAA.. The oligonucleotides used for PCR-amplified products covering the ρ6 coding sequence carried a Ndel site (bold) at the ATG codon, for direct ligation into the pET-H vectors. The primers used were: HVP6MA: AATTACATATGGCGGCCGCCGTTTCTGCC HVP6MF: AATTACATATGTTCGCGGCCGCCGTTTCT Each of these primers was used in conjunction with primer HVP6C2 to generate a PCR product of 0.2 kbp. These products were blunt-end hgated into vector pBSSK(-) which had been cut with EcoRV and dephosphorylated. SUBSTITUTE SHEET The insert corresponding to die p6 gene was excised with Ndel and BamHI (using the BamHI site in the primer HVP6C2) and hgated into the expression vector pET-llb, which had been cut witii the same enzymes. For expression at higher levels, the insert was transferred to PT7T2 as a Xbal - BamHI fragment, yielding plasmids pTP6MA and pTP6MF. IPTG induction of bacteria containing plasmids pTP6MA or pTP6MF were used produce p6 for bioassay. xiii-b) Expression of viral genes in E. cαli and bioassay in larvae Expression of P64 IPTG induction of bacteria containing plasmid pT7T2-P65, which contains an insert running from the location of primer HVPET65N to that of primer HVP65C, yielded a protein of molecular weight 68 000. This was 3 000 molecular weight greater than the size of the authentic coat protein, as expected. Expression of pP65G, which contains an insert running from HVPET63N to HVP65C, yielded a protein of 65 000 molecular weight. The authentic capsid protein (P64) was expressed poorly from plasmid pT7T2- P64. Recloning this insert as a Ndel-BamHI fragment back into the other form of the vector (PT7T2b) did not alter this. Expression of P70 IPTG induction of bacteria containing plasmid pT7T2-P70, which contains an insert running from the location of primer HVPET65N to that of primer HVP6C2, yielded a protein of molecular weight 73 000. This was 3 000 molecular weight larger than the size of the precursor of the coat protein, as expected. The authentic capsid protein precursor (P71) was expressed poorly from plasmid pT7T2-P71. Recloning this insert as a Ndel-BamHI fragment back into the other form of the vector (pT7T2b) did not alter this. SUBSTITUTE SHEET Due to the observation mentioned in vi) above, plasmids designed to express ah forms of the capsid proteins from several possible ATG's at the start of the open reading frame were constructed. It was found that both authentic P64 and P71 were expressed poorly in bacteria. In contrast, P17 and die forms of the capsid protein commencing at the P17 ATG were expressed very well. The extra C residue present in the latter two constructs resulted in a fusion protein being made from these expression plasmid. The sequence of the fusion proteins can be derived from Fig. 2 by including an extra C at position 570. The fusion caused die first 67 residues of the HaSV capsid protein to be replaced by the first 95 residues of P17. Good expression of the large capsid precursor and protein was achieved, but the size of these proteins were above 3 kDa larger than the autiientic forms. Notwithstanding tiiis the expression products of die vectors containing the 5C variant of RNA 2 are still useful because the resulting product, a P70 variant, is only modified at the NH 2 terminus. Since this terminus is thought to be embedded in the capsid structure and tiierefore not to participate in the initial interaction with the larval midgut cell, the variant is still useful. In order to produce constructs which ensure that the expressed proteins possessed the native amino terminus, new plasmids carrying the correct sequence were then cloned into the expression vector (pT7T2). It was found tiiese plasmids to express proteins of the correct size. The P6 has not yet been to expressed from the new constructs. No evidence has been found for processing of P70 to yield the mature proteins in bacteria, nor upon in vitro translation of synthetic full-length RNA 2. The P17 gene has also been cloned into the same vectors for expression and bio-assay. This protein accumulates well in bacteria upon induction, and electron microscopy analysis has shown it form spectacular honeycomb-like structures under the bacterial cell wall, completely surrounding the cell interior SUBSTITUTE SHEET (results not shown). The properties of this protein including its amino acid composition and ability to form tube-like structures when expressed in bacteria suggest that it may be an homolog of a gap junction protein. The latter is involved in forming the channels linking the cytoplasms of adjacent epithelial cells in the insect gut. P17 could then play a role in enlarging or forming these channels, thereby enabling cell-to-cell movement of the virus in the insect gut, analogous to the movement or spreading proteins encoded by plant RNA viruses. In order to ensure that the expressed proteins carried the native amino terminus the correct sequence has also been cloned into the expression vector (pT7T2). The vector had been very slightly modified to tiiat described above to introduce two novel restriction sited (for Aflll and Bglll) flanking die Shine-Dalgarno sequence. The resulting constructs have been found to be poor producers of the capsid proteins. The complete coding regions (which have been completely checked by re-sequencing) have therefore been recloned into the more satisfactory vectors. Results using these constructs suggest that the amino-teπninus of the capsid protein presents inherent difficulties in expression. These difficulties may be imposed by either the nucleotide sequence encoding the amino terminus, or the actual amino acid sequence itself. To discriminate between these possibilities, two types of mutants were made in die sequence encoding die amino terminal 5 residues of die HaSV capsid protein. These amino-terminal mutants are as follows: HVP71GLY CCCATATG GGCGATGCCGGCGTCGCGTCACAG Met Gly Asp Ala Gly Val Ala Ser Gin HVP71SER: CCCATATG AGC GAG GCC GGC GTC GCG TCA CAG Met Ser Glu Ala Gly Val Ala Ser Gin SUBSTITUTE SHEET Native HaSV seq: ATG GAG GAT GCT GGA GTG GCG TCA CAG Met Gly Asp Ala Gly Val Ala Ser Gin EXAMPLE 4 EXPRESSION IN BACULOVIRUS VECTORS AND BIOASSAY ON LARVAE Materials and Methods A(i) Cloning of HaSV capsid protein gene. The capsid protein gene was amplified by PCR using the foUowing primers: 5 * primers: HV17V71: 5' GGGGGATCCCGCGGATTTΔTGAGCGAG HV17E71: 5'GGGGGATCCCGCGGAGACΔIQAGCGAGCACAC HVP71: 5'GGGGGATCCAGCGACΔIGAGAGATGCTGGAGTGG HW71: 5 ' GGGGGATCCAGCGACΔ1GAGAGATGCTGGAGTGG The ATG triplets initiating P17 (in HV17V71 and HV17E71) or P71 (in HVP71 and HW71) are underlined) ■V primers: Primers HVP65C and HVPGC2, described in Example 3. Results section Xiiia, were used. These constructs were made using one of the four 5' primers and HVP6C2. Plasmids constructed from PCR products made using one of the four 5'- primers and HVP65C are called 17V64 (made using 5' SUBSTITUTE SHEET pri er 17E71), P64 (made using 5' primer P71) and V64 (made using 5' primer V71). These plasmids allow expression of P64. A(ii) Cloning a full length cDNA of HaSV RNA 1. For expression of an RNA transcript corresponding to full length HaSV RNA 1, in insect cells by baculovirus infection or plasmid transfection, PCR was used to generate a fragment of cDNA linking the 5' end of RNA 1 to a Bam HI site. The primers were: HVR1B5' 5' GGGGGATCCGTTCTGCCTCCCCGGAC (where the underlined nucleotide represents the start of natural RNA 1), and an ohgonucleotide complementary to nucleotides 1192 = 1212 of RNA 1. The template was plasmid pSRl(E3)B described in Example 3 above. A segment of the 1240 bp PCR fragment corresponding to the 5' 320 nucleotides of RNA 1 was excised with Bam HI and ASC II and cloned into the Bam HI site of pBSSK(-)[Stratagene] together with die 5 kbp ASCII - Bam HI fragment of pSRl(E3)B, giving plasmid pBHVRIB, which carries the complete cDNA to HaSV RNA 1, flanked by Bam HI sites. A(iii) Cloning a full length CDNA of HaSV RNA 2. For expression of an RNA transcript corresponding to full length RNA 2 in insect cells by baculovirus infection or plasmid transfection, plasmid pB +NR2B was made by inserting a fragment carrying Hind III and Bam HI sites from the multiple cloning site of vector pBSSK(-) [Stratagene] into plasmid pSR2B described above. The resulting plasmid, called pBHVR2B, carried the cDNA corresponding to full lengtii HaSV RNA 2, flanked by Bam HI sites. A(iv) Baculovirus transfer plasmids. Bam HI fragments of 5.3 and 2.5 kbp corresponding to HaSV RNA's 1 and 2 respectively, were excised from pBHVRIB and pBHVR2B respectively and SUBSTITUTE SHEET inserted into the baculovirus transfer vectors described below, which had been linearised with Bam HI. B. Baculovirus Expression of Proteins. Baculovirus transfer vectors and engineered AcMNPV virus were transfected into Spodoptera fmgiperda (SF9) cells as described by the supplier (Clontech) and as described in the foUowing references: Vlak, J.M. & Kens, RJ (1990) in 'Viral Vaccines", WUey-Liss Inc., NY, pp.92-128; Kitts, PA et al (1990) Nucleic Acids Research 1& 5667-5672; Kitts, PA and Possee, R.P. (in preparation); Possee, R.D. (1986) Virus Research, £: 43-59. C Western Blotting As in Example 1 D. Oligonucleotides. The foUowing Ribozyme Oligonucleotides were produced according to standard methods. HVRlCla 5' CCATCGATGCCGGACTGGTATCCCAGGGGG 5' HVR2Cla 5 ' CCATCGATGCCGGACTGGTATCCCGAGGGAC RZHDV1 5' CCATCGATGATCCAGCCTCCTCGCGGCGCCGGATGGGCA RZHDV2 5 ' GCTCTAGATCCATTCGCCATCCGAAGATGCCCATCCGGC RZHC1 5 ' CCATCGATTTATGCCGAGAAGGTAACCAGAGAAACACAC SUBSTITUTE SHEET RZHC2 5 ' GCTCTAGACCAGGTAATATACCACAACGTGTGTTTCTCT Results A series of recombinant baculoviruses has been constructed, based on die pVL941 transfer vector (PharMingen) or pBakPakδ (Clontech) and die AcMNPV. These are designed to express the correct forms of the precursor and processed HaSV capsid proteins (P64 and P71) as weU as the smaUer capsid protein P6, and P17. In aU systems where replicatable RNA encoding the nucleotide sequences of the present invention are to be used, such as eukaryotic systems, in order to get efficient rephcation, translation or encapsidation of the RNA it is necessary to excise structures downstream of the t-RNA like structure such as the 3' extension or poly A taU on the RNA. In order to carry out such an excision, ribozymes or other suitable mechanisms may be employed. This self cleavage activity of the ribozyme containing transcript should proceed at such a rate that most of the transcript is transported into the cytoplasm of the ceU before the regeneration of a replicatable 3' end occurs. Such ribozyme systems are more fuUy explained in Example 7. In the results presented here highly efficient production of P64 and P71 has been achieved. Electron microscopy and density gradient analysis have confirmed tiiat empty particles ("capsoids") are being produced in infected cells that efficiently express the P71 precursor gene. PI 7 placed in die context of the H. vireycetisjuveinle hormone esterase (JHE) gene (Hanzlik T.N., et al, J. Biol. Chem. 264, 12419-25 (1989)) is produced, but not in large amotmts. The latter construct results in a reduction of expression of the capsid protein from the same recombinant, presumably due to a reduction in the number of ribosomes reaching the AUG for the capsid gene. SF9 cells infected with recombinant baculovirus have been shown to contain large amounts of icosahedral virus particles by electron microscopy (data not shown). These particles contained no RNA and were empty inside. This observation shows that signals on the viral RNA required for encapsidation of SUBSTITUTE SHEET ! RNA must be located in eitiier the 5' 270 nucleotides or the 3' 170 nucleotides, or botii, since these sequences were missing from the RNA transcripts made using recombinant baculovirus. Expression of HaSV proteins was confirmed by Western blotting of total protein extracts from infected insect cells. In addition, the pAcUW31 vector (Clontech), which carries two promoters, is being used to simultaneously express p6 and p64 as separate proteins. In order to bioassay the capsid protein produced in baculovirus infected ceUs, it is first necessary to purify it from the baculovirus expression vector. Preliminary attempts have made use of density gradients, based on the observation that empty virus particles ("assembled capsids") are in fact produced in infected cells. As outlined earlier, the HaSV genome or portion thereof is a particularly effective insecticidal agent for insertion into baculovirus vectors. Such a vector is constructed by insertion of the complete virus genome or portion thereof (preferably the replicase gene) into the baculovirus genome as shown in Fig. 13. Preferably the virus genome or rephcase is transcribed from a promoter active constitutively in insect cells or active at early stages upon baculovirus infection. An example of such a promoter is the heat shock promoter described in Example 7. Heat shock promoters are also activated in stressed cells, for example cells stressed by baculovirus infection. An even more preferable use of such a baculovirus construct is to use the HSP promoter to drive die HaSV rephcase and another gene for a toxin (as exemplified elsewhere in the specification) where the RNA expressing the toxin gene is capable of being rephcated by die HaSV replicase. Such recombinant baculoviruses carrying the HaSV genome or portions thereof for expression in larvae at early or other stages of the baculovirus infection cycle are particularly effective biological insecticides. SUBSTITUTE SHEET EXAMPLE 5 EFFECT OF HaSV GENES AND THEIR PRODUCTS ON PLANTS Materials and Methods A. Electroporation of protoplasts. Protoplasts of Nicotiana tobacum, N. plumbagin olia and Triticum aesticum and oats were produced and electroporated witii either HaSV or HaSV RNA as described in Matsunaga et al (1992) J.Gen. Virol. 1__ 763-766. B. Northern blot analysis - RNA extraction from protoplasts after harvest The protoplasts are subjected to 3 cycles of freezing and thawing, and tiien an equal volume of 2x extraction buffer (100 mM Tris-HCI, pH 7.5, 25 mM EDTA 1% SDS, made in DEPC treated water) is added, foUowed by 1 volume of phenol (equilibrated in 10 mM Tris-HCI pH 8.0) heated to 65 °C. The samples are mixed by vortexing and incubated at 65 β C for 15 min, vortexing every 5 min. After phase separation by centrifugation at room temperature for 5 min, the aqueous phase is re-extracted with phenol, re separated by centrifugation and re-extracted witii chloroform/isoamyl alcohol. To the aqueous phase are then added 0.1 volume of DEPC-treated sodium acetate (pH 5.0) and 2 volumes of ethanol. The RNA is recovered by precipitation at -70 °C, foUowed by centrifugation at 4 °C for 15 min. The samples were then analysed by agarose gel electrophoresis as described in example 1. After blotting to Zeta-Probe membrane (BioRad), die hybridization protocols were as above for Example 2. C. Total protein from HaSV - electroporated protoplasts. Protoplasts were analysed by SDS-polyacrylamide gel electrophoresis and Western blotting as described in Example 1. SUBSTITUTE SHEET Results i) Use of complete (replication-competent) RNA virus genome in protoplasts a) HaSV replication in protoplasts The nodavirus FHV has previously been shown to replicate in barley protoplasts (Selling H.H., Allison, R. F. and Kaesberg, P. Proc. Natl. Acad. Sci. USA 87,434-8 (1990). To determine whether HaSV virus RNA can replicate in plants protoplasts, when introduced by electroporation, experiments using protoplasts from Nicotiana plumbagin oli and wheat have been conducted. (These are aU species for which protoplasts are regularly avaUable in the Division of Plant industry). Assays for rephcation including RNA (Northern) blots using probes derived from cloned fragments of cDNA to RNAs 1 and 2, and Western blots, using the antiserum to purified HaSV particles. Initial experiments showed that both HaSV virus and RNA electroporated into protoplasts of N. plumbaginξolia resulted in HaSV rephcation as studied using and verified by northern blots and ELISA As a positive control TMV RNA was electroporated and was rephcation observed. b) Bioassays Protoplasts into which HaSV RNA had been introduced by electroporation were harvested after 6 or 7 days post electroporation and used in bioassays on neonate larvae by addition to normal diet. The results showed significant stunting of test larvae in comparison to control larvae (see Table 1 below). Protoplasts lacking HaSV RNAs had no effect on the larvae, confirming the result of control experiments. This result confirms that HaSV RNA when expressed or replicated in plant cells, is able to cause the formation of infectious virus particles able to control insect larvae feeding on d i e plant material. Northern blotting has been used to confirm tiiat RNA electroporation into protoplasts leads to RNA rephcation. SUBSTITUTE SHEET 60 Table 1: Results of Bioassay from a typical experiment with Nicotiana and oat protoplasts (oat results are shown in brackets) [see over] Number stunted 1. 0/10 (0/9) 2. 0/12 (0/11) 3. 12/12 (11/11) 4. 12/12 (n.d.) 11/12 (10 * /12) 5. HaSv rephcation in the larvae was confirmed except for two larvae which were dead. The letters "n.d." mean the experiment was not done. The above results demonstrate assembly of HaSV particles from electroporated RNA in protoplasts of both moncot and dicot plant species. c) Plasmids to test rephcation of cloned and engineered forms of HaSV (1) Plasmids aUowing in vitro transcription of HaSV RNAs 1 and 2 for electroporation into protoplasts have already been described above. (2) Plasmids for transient expression of individual HaSV RNAs (1 or 2) in protoplasts. FuU-lengdi cDNAs for the two viral RNAs have been inserted into expression plasmids pDH51 (with the CaMV 35 S promoter. Pietrzak M., et al (9186) Nucl. Acids Res. 14, 5857-68) for dicots and pActLcas (with the rice actin promoter) for monocots (McElroy et al (1990) The Plant CeU 2. 163- 171). As with die vectors for expression in insect cells, these expression plasmids are being modified to include a cis-acting ribozyme for generation of authentic ends. The non-ribozyme plasmids gave no virus rephcation. ii) Expression of capsid protein in plants In view of the present inventors' observation that empty particles ("assembled capsids") are being produced in baculovims-infected ceUs that efficiently express the P71 precursor gene, expression of the coding region for the capsid protein in tobacco plants was investigated. The vector chosen for this purpose is based on pDH51 which carries the CaMV 35S promoter and polyadenylation SUBSTITUTE SHEET signal. If necessary for improved expression, this vector can be modified by the addition of a translation enhancer sequence from e.g. TMV. Although certain groups have constructed transgenic plants expressing the capsid proteins of plant viruses, there has been only one recent report of assembly of empty capsids in such plants (Bertioh et al.,(1991) J. gen. Virol. 72: 1801-9). Bertioh et al point out that the protein-protein interactions in most icosohedral plant RNA viruses may be too weak to aUow assembly of such capsids. In addition to the present inventors' observation of empty HaSV capsids, it has been found these capsids are very tough, showing great resUience to e.g. repeated cycles of freezing and thawing, so that it is expected to see assembly of empty HaSV capsids ("assembled capsids") in transgenic plants. EXAMPLE 6 IDENTIFICATION OF MIDGUT BINDING DOMAINS Materials & Methods A Plasmid construction Was as described in Examples 3 and 4. B. Western blotting Was as described in Examples 1 and 3. C. Invitro translation In vitro transcripts of cloned CDNA of HaSV RNA's was translated in vitro as in Examples 1 and 3. D. Preparation of Brush Border Membrane Vesicles. Brush Border Membrane Vesicles were prepared from freshly isolated larvae midguts of H m Armigera y the method of M.Wolfersberger et al (1987) Comp. Biochem. Physiol. 86Δ: 301-308, as modified by S.F.Garczyuski et.al. (1991) Applied Environ. Micro-biol 52; 1816-2820. Brush Border Membrane Vesicles binding assays using invitro labeUed protein or 125 I-labeUed protein were as SUBSTITUTE SHEET described in Garczynski et.al. (1991) or in H.M.Horton and Burand, J.P. (1993) J.Virol. 62; 1860-1868. Results i) Determination of epitopes on the capsid surface Comparison of the recently published sequence of die Nudaurelia ω virus (NωV) capsid protein with tiiat of HaSV shown that these proteins are closely related and f aU into four distinct domains, which are alternatively variable and highly conserved. These domains are summarised as foUows: Residues: % identity Comparison of this observation with the alignment by Agrawal and Johnson (1992) between the NωV and die nodavirus BBV (whose crystal structure is known: Hosur et al (1987) Proteins: Structure, Function & Genetics 2 167- 176) showed tiiat the variable region coincided with a region forming the most prominent surface protrusion on the BBV capsid. Botii HaSV and NωV carry large insertions at this point relative to BBV, and these insertions are largely different in sequence. Assuming that the alignment by Agrawal and Johnson (1992) is correct, then this means that HaSV and NωV have a more prominent pyramid-like structures as a surface protrusion than do the nodaviruses, and die pyramid-like structures are different. As already noted, there is no immunological cross-reactivity between the two viruses, despite die high degree of identity. There is thus a strong implication of the variable domain as a surface protrusion which functions as the sole antigenic region. To confirm this a 400 bp Νarl fragment spanning the variable region was deleted from the capsid gene in die expression vector. With end-fiUing of these sites the deletion is in-frame, so that a truncated protein of ca. 57 KDa is produced in bacteria upon induction. This protein was recognized only poorly SUBSTITUTE SHEET on Western blots by the antiserum against intact HaSV particles made in rabbits. The central variable domain was recognized weU by the antiserum when expressed in isolation from the rest of the capsid gene. As shown in the table above the region of HaSV capsid protein comprising residues 273-439 shows great divergence form the corresponding region of die NωV capsid protein, compared to its immediate flanking regions. Within this region an especiaUy divergent domain is found from residue 351 to residue 411, which shows only 25% identity to die corresponding region of die NωV capsid protein. This region is flanked by the sequences corresponding to die β-sheet structural features β-E(residues 339-349) and β-F(residues 424-431) of the HaSV capsid protein, based on the alignment the NωV and nodavirus capsid proteins by Agrawal and Johnson (1992), and is therefore likely to form the loop of die most prominent surface protrusion on the HaSV capsid. This is based on comparison and correspondence to the nodavirus capsid protein structure and capsid structure as described by Wery J.-P. and Johnson, J.E. (1989) Analytical Chemistry 61, 1341A-1350A and Kaesberg, P., et al. (1990) J. Mol. Biol. 214, 423-435. This loop is thought to contain important epitopes. It is significant that this exterior loop on the nodavirus capsid protein is one of the most variable regions when capsid proteins sequences from a number of nodaviruses are compared (Kaesberg et al. 1990). FinaUy, the present inventors have observed a significant level of immunological cross-reaction on Western blots, between antisera against the CryΙA(c) Bt toxin and HaSV capsid protein, whether obtained from virus or expressed in bacteria. Initial data from the Narl deletion mutant described above suggest that this binding is not to the central variable domain, but to otiier regions of the capsid protein. The only other region of the proteins which shows extensive sequence variability, the amino terminus, cannot be responsible for the binding, since both authentic capsid protein and die protein with an altered amino terminus expressed in bacteria are recognized by die anti Bt antisera. SUBSTITUTE SHEET ii) In-Vitro binding assays The fuU-length clones for in vitro translation yielding highly ^S or 3 H labeUed proteins were constructed by replacing the bacterial translation interaction signal in the T7 plasmids above by the more active eucaryotic context sequence from the JHE gene. The labeUed capsid protein made by in vitro translation of the in vitro transcripts may be tested for binding to brush border membrane vesicles (BBMVs). Conditions are optimised by testing different procedures. The deletion mutant lacking approximately 125 amino acids in the central region, and containing the variable domain, as weU as others derived from it are also tested. iii) Fusion proteins comprising virus capsid midgut binding domains and other proteins The idea behind these tests is to fuse the binding domain from the HaSV capsid protein to either large proteins (preferably indigestible, causing protein to aggregate in or on the midgut cells) or toxin domains from other proteins with suitable properties but normaUy different binding specificities (e.g. Bt). In initial experiments, the gene for die complete capsid protein has been fused to die GUS gene, as has a deletion mutant containing essentiaUy only the central portion of the capsid gene. The resulting fusion proteins are being expressed in bacteria and tested for GUS activity, and makes mem sensitive probes for binding experiments on midgut tissue. iv) Mapping binding sites using Bt HaSV fusion proteins Analysis of deletion mutants of the CryΙA(c) Bt toxin has identified domains which may be involved in determining die host-specificity of this Bt by acting as receptor-binding sites (Schnepf et al (1990) J. Biol. Chem. 265: 20923-20930; Li et al (1991), Nature 353: 815-21. The present inventors have obtained a clone of this toxin gene. Deletion mutants corresponding to those identified by Schnepf et al are constructed. Segments of the HaSV capsid protein gene can SUBSTITUTE SHEET then be inserted into these mutants, the protein expressed in bacteria and their insecticidal function assayed. EXAMPLE 7 VIRAL GROWTH IN CELL CULTURE Materials & Methods A Cell Lines The foUowing cultured insect ceU lines were tested for infection by HaSV: DmsophUa melanogaster, Helicoveφa armigera (ovarian derived), Heliothis zea (ovarian derived), Plutella xylostella, Spodoptera frigiperda (SF9). AU lines were grown under standard conditions. Upon reaching confluence, the culture medium was removed and aU mono-layers covered with 1.5 ml of ceU culture medium into which HaSV had been dUuted; die average multiplicity of infection (M.O.I.) was 10 4 . After adsorption at 26 °C for 2h, the inoculum was removed, the cells carefuUy washed twice with phosphate buffered saline (pH 7.0) and incubation continued with 5 ml of 10%. Foetal calf serum in TC199 culture medium (Cyto Systems). B. Northern Blotting Analysis. Virus rephcation in aU the above ceU lines was confirmed by northern blotting analysis. Total RNA was extracted from infected cells by the method of Chomczynski and Sacchi (1987). Anal. Biochem. 162; 156-159. The ceUs were lysed in 1 ml of lysis solution (4M guanidinium thiocyanate, 25mM sodium citrate, pH 7, 0.5% sarcosyl, 0.1M 2-mercaptoethanol). In order, 0.1 ml of 2M sodium acetate, pH 4, 1 ml of phenol (0.2M sodium acetate equUibrated), and 0.2 ml of chloroform-isoamyl alcohol mixture (49:1) were added with thorough mixing between reagents. This was then vortexed for 10 s and cooled on ice for 15 min. Tubes were centrifuged in an Eppendorf centrifuge at 14k for 15 min at 4 β C for at least 15 min to aUow RNA precipitation. RNA was peUeted by centrifugation at 14k for 15 min, washed with 0.6 ml of ice-cold 70% ethanol, peUeted once again (10K, 10 min), air dried at room temperature and resuspended in DEPC (Sigma) treated miUipore water. RNA was subject to SUBSTITUTE SHEET denaturing agarose gel electrophoresis in the presence of formaldehyde according to Sambrook et.al. (1989). The gel was Northern transferred to a zeta-probe membrane (Biorad) as described by Sambrook et.al. (1989). The probe was prepared by random-priming the 3' sequences of the HaSV genome using DNA and cDNA clones pSHVR15GB and P T7T2p71SR-l as per manufacturer's instructions (Boehringer-Mannheim). Hybridization was carried out as described for the standard DNA probe protocol contained widiin the literature for the zeta-probe membrane (Biorad). C Vectors Vectors as described below. Results It has been found that HaSV wiU replicate in several continuous ceU lines, of which the best is the Spodoptera fmgiperda hne SF9. Time course assays by Northern blotting in SF9 cells have shown that RNA 1 rephcation is clearly detectable within a few hours of infection. RNA 2 is present only in very smaU amounts early in infection and accumulates much more slowly than RNA 1 does. This observation is consistent with one made earlier in HaSV-infected larvae, where RNA2 replication was not observed until 3 days after infection. Some apparent replication was also observed in DrosophUa cells (DL2), but with the difference that more RNA 2 replication was observed at the early time points compared to the lepidopteran ceU lines above. Plasmids that express the HaSV genome as RNA transcripts from full length cDNA clones have been constructed and tested. These clones, constructed by PCR and carefuUy checked, have restriction sited immediately adjacent to the ends of the sequence. Transcription is driven from a specially-re-engineered DrosophUa HSP70 promoter. SUBSTITUTE SHEET i) Constructs for expression in insect cells The constructs are based on vectors carrying the DrosophUa HSP or actin promoters and suitable polyadenylation signals from DrosphUa (Corces & PeUicer (1984) J. Biol. Chem. 259: 14812-14817) or SV40 (Angelichio et al (1991) Nucl. Acids. Res. 1& 5037-5043). Since transcription from such plasmids generates viral RNAs carrying long 3' terminal extensions derived from sequences in the poladenylation signal fragment, it is necessary to achieve cleavage of the transcript immediately after the 3'sequence of the viral RNA. These plasmids gave no virus replication, presumably because of the 3' terminal extension. The method of choice for obtaining authentic 3' termini is based on introduction of DNA sequences encoding a cis-acting ribozyme into the constructs. With suitable engineering, such a ribozyme wUl cleave immediately 3' to the viral sequences within the transcript. Suitable ribozymes, based on die hepatitis delta virus (Been M.D., Perrotta, A T. & Rosenstein, S.P. Biochemistry 31, 11843-52 (1992) or the hairpin cassette ribozyme (Altschuler, M., Tritz R. & Hampel, A Gene 122, 85-90 (1992) have been designed. This involves synthesis of overlapping oligonucleotides, which are then annealed and end-fiUed with the Klenow fragment of DNA polymerase, to create short DNA fragments encoding the desired ribozyme. These fragments carry restriction sites at ti eir termini aUowing them to be ligated into plasmids between the viral RNA cDNA (which has a 3' restriction site added by PCR) and the restriction fragment carrying the poladenylation signal. EXAMPLE 8 SHEDDING OF INFECTED CELLS Materials & Methods A Confocal Laser Scanning Microscopy. (CLSM) CLSM enables the visualisation and analysis of three-dimensional ceU and tissue structures at the macro and molecular levels. The Leica CLSM used in this example is based on an MC 68020/68881 VME bus (20MHz) with standard 2Mbyte framestore and 4Mbyte RAM and OS9 operating system with programmes written in C code. It incorporates a Leica Diaplan research SUBSTITUTE SHEET microscope and using X10/0.45, X25/ 0.75 ^ X40/ 1.30 and X63/1.30 Huotar objectives has a claimed optical efficiency better than 90%. The confocal pinhole is software controUed over the range of 20 to 200 μm. Excitation at 488 and 514 nm is provided by a 2 to 50 mW argon-ion laser. B. Immunocytochemistry (ICC). For whole mount ICC, tissues were dissected under saline and fixed in fresh 4% formaldehyde in phosphate buffered saline (PBS) for at least 15 mins. After multiple washes in PBS they were permeablized either by 60 mins incubation in PBT (PLBS witii 0.1% Triton X-100 plus 0.2% bovine serum albumin). After 30 mins blocking in PBT+N (5% normal goat serum) tissue was incubated in primary antibody dUuted (1:40) in PBT+N for at least 2 hrs at room temperature then at 4 °C overnight. After extensive washing in PBT and 30 mins blocking in PBT+N the FITC conjugated secondary antibody dUuted (1:60) in PBT+N was incubated for 2 hrs at room temperature plus overnight at 4 β C. After multiple washes in PBT and PBS the tissue was cleared in 70% glycerol and mounted in 0.01 %w/v p-phenylenediamine (Sigma#P1519) dissolved in 70% glycerol. AU processing was at room temperature unless otherwise stated. Results The inventors' current model for the effect of HaSV involves the detection by the insect midgut of infected cells, their identification as infected and their subsequent shedding in numbers sufficient to cause irreparable damage to the insect midgut. The evidence for this is based on the above and on the foUowing direct observation of the fate of infected cells in midgut tissue over 1-3 days post infection. These results in repeat experiments were complicated by the discovery tiiat anotiier unrelated virus was present in the larval population being tested. Preliminary findings indicated tiiat HaSV infection activates or fadlitates pathogenesis of the unrelated virus and together these cause severe disruption of the larval gut cells. Thus these two agents appear to act synergisticaUy in causing gut ceU disruption. SUBSTITUTE SHEET Midguts from larvae infected with HaSV were treated with the antiserum to purified HaSV particles (above) and examined under die Laser confocal microscope (described above). This established that some midgut cells were sufficiently infected with HaSV to give strong fluorescence signals. Such ceUs were moreover clearly separating from the surrounding tissue, a sign that they were in the process of being shed. Sim ar observation have been made with other insect viruses (Fhpsen et al (1992) Society for Invertebrate Pathology Abstract #96) although in these cases the effect is too localised and weak to cause any anti-feeding effect apparently only the smaU RNA virus of the tetraviridae which are localised to die gut and cause more-or-less severe anti-feeding effects in their hosts (Moore, N.F. in Kurstak E. (Ed) (1991) Viruses of Invertebrates. Marcel Dekker, New York pp277-285) are capable of such an effect to an extent sufficient for pest control. FoUowing on from the immune-fluorescence work, in situ hybridization can be carried out to detect RNA rephcation in infected cellsPurthermore, larvae infected with a recombinant HaSV expressing a foreign gene at early stages (by insertion of that gene into RNA 1 in place of the N-terminal portion of the replicase gene) can be studied. A correlation between virus rephcation and cell rejection can be confirmed by histochemical analysis of the midgut ceUs of the infected larvae. Thus the ceU-shedding phenomenon offers a direct and rapid assay for early events in HaSV-infected gut tissue. Extracts of baculo-vector infected insect cells carrying empty HaSV particles can be fed to larvae directly and die midgut examined by toluidine blue staining and immune- fluorescence at intervals after infection. This wiU aUow direct determination of whether the particles can bind the brush border membranes in intact gut, and whether such binding can induce tiie massive disruption evident in normaUy infected larvae. Control experiments using extracts from cells infected with the baculovector alone can be conducted to observe and distinguish effects due to die vector. The immune-fluorescence assay on midgut tissue aUows analysis of SUBSTITUTE SHEET binding to midgut brushborder membranes. Once determined for wUd-type capsid protein expressed from a baculo-vector, deletion or replacement mutants can be inserted into the baculovectors. Suitable ceU extracts from these can be used to infect larvae. EXAMPLE 9 ENGINEERED VIRUS AND USES Materials & Methods (as indicated in earlier Examples) i) Engineered virus as a vector for other toxin genes This involves placing suitable genes under control of HaSV rephcation and encapsidation signals. Genes which may be suitable include intraceUular insect toxins such as ricin, neurotoxins, gelonin and diphtiieria toxins. The toxin gene may be placed in d e viral gene such that it is a sUent (downstream) cistron on a polycistronic RNA or in a minus strand orientation, requiring rephcation by the viral polymerase to be expressed. Standard techniques in molecular biology can be used to engineer these vectors. A discussion of two recombinant HaSV vectors which have been designed is given below: for RNA 1: The reporter gene (or one of the toxin genes mentioned above) is inserted in place of die amino-terminal portion of the putative replicase gene, such that the intiation codon used for the replicase (ie that at nucleotides 37-39 of die sequence) is now used to commence reporter gene translation. The fusion is achieved by die use of artificial Ncol restriction sites common to both sequences. The short 36 nucleotide 5'-untranslated leader of RNA 1 (shown in upper case) is synthesised as the foUowing sequence: ggggatcεacaGTTCTGCCTCCCCCGGACGGTAAATATAGGGGAACCATG Gtciagagg, SUBSTITUTE SHEET using two overlapping oligonucleotides comprising the first 40 nucleotides and die complement of the last 40 nucleotides respectively. These primers are annealed and end-fiUed by Klenow. The resulting fragment is then cut with BamHI and Xbal (sites underlined) and cloned with plasmid vector pBSIISK(-). The GUS gene carrying a Ncol site at the ATG codon was obtained as a Ncol- SacI fragment from plasmid pRAJ275 (Jefferson, RAJ Plant Mol. Biol. Rep 5, 3387-405 (1987)). This Sa site is located just downstream from the coding sequence for the GUS gene. The 5' leader of RNA is excised as a BamHI-NcoI fragment from the above vector, and is hgated together with the NcoI-SacI fragment carrying the GUS gene into plasmid pHSPRIRZ or pDHVRIRZ carrying the fuU-length cDNA insert of RNA 1 (see above) which has been cut with BamHI and Sad. The resulting plasmid then carries a complete form of RNA 1 but with the amino- terminal portion of the rephcase gene substituted by the GUS gene. It is desirable to produce a construct with approximately the same size as RNA 1 for encapsidation purposes. SimUar approaches are adopted for RNA 2, with d e foreign, reporter or toxin gene fused to die initiation codon of either P17 or P71. In either case the context sequence of the introduced gene is modified to give the necessary expression level of that protein. The foreign gene is introduced into plasmids pHSPR2RZ or pDHVR2RZ. The above recombinants have been described specificaUy as insertions of a reporter gene (GUS). The toxin genes to be inserted are described on page 13 of die specification. These preferably further require a signal peptide sequence added at the amino-terminus of the protein. SUBSTITUTE SHEET ii) Capsid technology Identification of encapsidation (and replication) signals on virus RNA aUows design of RNAs which can be encapsidated in HaSV particles during assembly of virus in a suitable production system. The virus capsids then carry the RNA of choice into the insects midgut cells where the RNA can perform its intended function. Examples of RNAs which may be encapsidated in this manner include RNAs for specific toxins such as intraceUular toxins, such as ricin, gelonin, diptheria toxins or neurotoxins. This strategy is based on die resistance of the virus particle to the harsh gut environment. iii) Other uses of the capsid particle The capsid particles can be used as vectors for protein toxins. Knowledge of icosahedral particle structure elucidated by die inventors suggests that die amino and especiaUy the C-termini are present within the capsid interior. It is possible to replace or modify die amino acid sequence corresponding to P7 such tiiat it encodes a suitable protein toxin which is cleaved off the bulk of the capsid protein during capsid maturation. As with toxin-encoding mRNAs, the HaSV capsid delivers it to die midgut ceU of die feeding insect, where it exerts the desired toxic effect. iv) Use of HaSV in plants The use of HaSV in the production of insect-resistant transgenic plants are shown in Fig. 12. These inventions are based on tiie use of either the complete HaSV genome, or of the rephcase gene as a tool for the amplification of suitable amplifiable mRNAs (e.g. encoding toxin) or of the capsid protein as a means to deliver insecticidal agents. These strategies are now described in some detaU. a) Use of die complete HaSV genome Fragments of cDNA corresponding to the fuU-length HaSV genome components RNAs 1 and 2 are placed in a suitable vector for plant transformation under the control of either a constitutive plant promoter (e.g. SUBSTITUTE SHEET D the CaMV 35S promoter mentioned above) or an inducible promoter or a tissue specific (e.g. leaf-specific) promoter. The cDNAs are foUowed by a cis- cleaving ribozyme and a suitable plant polyadenylation signal. Transcription and translation of these genes in transgenic plant tissues and cells leads to assembly of fuUy infectious virus particles to infect and kUl feeding larvae. A variation on this strategy is to remove from the cDNA for RNA2 the fragments encoding RNA encapsidation and/or replication signals. This results in either the assembly in the plant cells of HaSV particles carrying only RNA 1, or of HaSV particles carrying RNA 1 and a form of RNA 2 which cannot be rephcated in the infected insect ceU. A further variation on this strategy is to modify die plant transgene encoding RNA 2 so that it is stiU replicatable and encapsidatable, but no longer express functional capsid protein. HaSV capsids made in such plant cells wiU then be capable of making both the replicase and P17 in infected insect cells, but not of assembling progeny virus particles therein (such as shown in Fig. 12(d)). These measures confer inherent biological safety in the form of containment on the use of such transgenic plant material. (b) Use of portions of HaSV genome to deliver toxins to insect ceUs This approach makes use of any of the systems described in (a) above. Plant cells contain an additional transgene encoding a suitable insect-specific, intraceUular toxin (as described above). Such a toxin gene is expressed by plant RNA polymerase in either a positive or a negative sense (the latter is preferred) and in such a form that die RNA can be encapsidated by HaSV capsid protein and/or replicated by die HaSV replicase in infected insect ceUs (see Figs. 12a and 12b) Transgenic plants would contain two different transgenes, making either unmodified capsid protein precursor or a modified form in which most of the carboxyterminal protein P7 is replaced by a suitable insect-specific toxin or one SUBSTITUTE SHEET which is inactive as part of a fusion protein. (Gelonin or other ribosome- inactivating proteins, insect gut toxins or neurotoxins may be suitable here.) Expression from these two transgenes would be regulated so tiiat only the required amounts of the modified and unmodified forms are made in the plant ceU, and assembled in such proportions into the capsoids. One way to modulate the production of capsotixin fusion proteins is to make translation of the carboxyterminal toxin reading frame dependent on a translational frameshift or read-tiirough of a termination codon. Witii an appropriate low frequency of frame-shifting (eg 0.1 - 2%), it could even be sufficient to use a single transgene, if it were possible to synthesise the P7 portion and die toxin portion as overlapping genes. Upon assembly (which we have demonstrated in insect cells using the baculovirus vectors) and maturation, the protein precursors are cleaved and release the mature P7 and die toxin, which remain within the capsoids. These proteins are not released until capsoid disassembly occurs in insect gut cells. The processed form of the toxin is then able to kiU the pest. (c) HaSV particles devoid of nucleic acid carrying one or more suitable protein toxins and/or their mRNA A protein toxin (or toxins) is expressed as a fusion with the capsid protein. The fusion protein then assembles into capsid carrying the toxin(s). These capsids present in the plant tissue exert an antifeeding effect on insects attaching the plant. EXAMPLE 10 EXPRESSION OF Hasv IN OTHER DELIVERY VECTORS Materials & Methods (as indicated in earlier Examples) Constructs simUar to those for plant expression are introduced into yeast or bacteria by standard techniques. Virus particles are assembled for eitiier fuUy infectious virus or any of the modified or biologicaUy contained forms described in Example 9. Microbes produced in suitable fermentation or j SUBSTITUTE SHEET culture f acihties and carrying such forms of the virus are then delivered to die crop by spraying. The microbial ceU waU provides extra protection for the virus particles produced within the microbe. WeU established techniques exist for culture and transformation of yeast (Ausubel, F.M. et al (eds) Current Protocols in Molecular Biology. J. Whey & Sons, NY, 1989). An example of a yeast expression vector is pBM272, which contains the URA3 selectable marker (Johnston, M. & Davies, R.W. Mol. CeU. Biol. 4, 1440-8, (1984); Stone, D. & Craig, E. Mol. CeU. Biol. 10, 1622-32 (1990). Another example of an expression vector is pRJ28, carrying the Trpl and Leu2 selectable markers. Yeast has recentiy been shown to support replication of RNA rephcons derived from a plant RNA virus, brome mosaic virus (Janda, M. & Ahlquist, P. CeU 72, 961-70 (1993). Since the BMV replicase is distantiy related to tiiat of HaSV, and the two viruses are likely to rephcate by simUar strategies within cells, yeast cells probably contain aU the ceUular factors required for HaSV to generate infectious virus. For bacteria, suitable expression vectors have been described above. SUBSTITUTE SHEET SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: Commonwealth Scientific and Industrial Research Organisation and Pacific Seeds Pty. Ltd. (ia) INVENTORS: P. D. CHRISTIAN, K. H. J. GORDON and T. N. HANZLIK (ii) TITLE OF INVENTION: INSECT VIRUSES AND THEIR USES IN PROTECTING PLANTS (iii) NUMBER OF SEQUENCES: 52 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: DAVIES COLLISON CAVE (B) STREET: 1 LITTLE COLLINS STREET (C) CITY: MELBOURNE (D) STATE: VICTORIA (E) COUNTRY: AUSTRALIA (F) ZIP: 3000 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Floppy disk (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: Patentin Release #1.0, Version #1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: 13 AUGUST 1993 (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: JOHN M. SLATTERY (B) REGISTRATION NUMBER: NA (C) REFERENCE/DOCKET NUMBER: 1613611 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: (613) 254 2777 j SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 13 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:l: GGATCCACAG NNN 13 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: ATGGGCGATG CCGGCGTCGC GTTCACAG 28 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: ATGGAGGATG CTGGAGTGGC GTCACAG 27 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: ATGAGCGAGG CCGGCGTCGC GTCACAG 27 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 30 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: CCATCGATGC CGGACTGGTA TCCCAGGGGG 30 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: CCATCGATGC CGGACTGGTA TCCCGAGGGA C 31 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: CCATCGATGA TCCAGCCTCC TCGCGGCGCC GGATGGGCA 39 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8; GCTCTAGATC CATTCGCCAT CCGAAGATGC CCATCCGGC 39 r SUBSTITUTE SHEET 79 (2) INFORMATION FOR SEQ ID NO:9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9: CCATCGATTT ATGCCGAGAA GGTAACCAGA GAAACACAC 39 (2) INFORMATION FOR SEQ ID NO:10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 39 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10: GCTCTAGACC AGGTAATATA CCACAACGTG TGTTTCTCT 39 (2) INFORMATION FOR SEQ ID NO:11: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 45 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11: GGGGGGAATT CATTTAGGTG ACACTATAGT TCTGCCTCCC CGGAC 45 (2) INFORMATION FOR SEQ ID NO:12: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12: GGGGGGATCC TGGTATCCCA GGGGGGC 27 j SUBSTITUTE SHEET ( 2) INFORMATION FOR SEQ ID NO: 13 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13: CCGGAAGCTT GTTTTTCTTT CTTTACCA 28 (2) INFORMATION FOR SEQ ID NO:14: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 46 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14: GGGGGATCCG ATGGTATCCC GAGGGACGCT CAGCAGGTGG CATAGG 46 (2) INFORMATION FOR SEQ ID NO:15: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 52 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15: AAATAATTTT GTTACTTTAG AAGGAGATAT ACATATGAGC GAGCGAGCAC AC 52 (2) INFORMATION FOR SEQ ID NO:16: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 55 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16: AAATAATTTT GTTTAACCTT AAGAAGGAGA TCTACATATG CTGGAGTGGC GTCAC 55 j SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO: 17 : ( i ) SEQUENCE CHARACTERISTICS : (A) LENGTH: 30 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS : single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17: GGAGATCTAC ATATGGGAGA TGCTGGAGTG 30 (2) INFORMATION FOR SEQ ID NO:18: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18: GTAGCGAACG TCGAGAA 17 (2) INFORMATION FOR SEQ ID NO:19: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 31 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19: GGGGGATCCT CAGTTGTCAG TGGCGGGGTA G 31 (2) INFORMATION FOR SEQ ID NO:20: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 28 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20: GGGGATCCCT AATTGGCACG AGCGGCGC 28 SUBSTITUTE SHEET 82 (2) INFORMATION FOR SEQ ID NO:21: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 29 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21; AATTACATAT GGCGGCCGCC GTTTCTGCC 29 (2) INFORMATION FOR SEQ ID NO:22: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 29 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22: AATTACATAT GTTCGCGGCC GCCGTTTCT 29 (2) INFORMATION FOR SEQ ID NO:23: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 19 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein - N terminal (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23: Phe Ala Ala Ala Val Ser Ala Phe Ala Ala Asn Met Leu Ser Ser Val 10 15 Leu Lys Ser SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:24: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 20 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ( ii ) MOLECULE TYPE: protein - internal (xi ) SEQUENCE DESCRIPTION: SEQ ID NO: 24 : Pro Thr Leu Val Asp Gin Gly Phe Trp He Gly Gly Gin Tyr Ala Leu 1 5 10 15 Thr Pro Thr Ser 20 (2) INFORMATION FOR SEQ ID NO:25: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 6 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein - internal (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25: Phe Ala Ala Ala Val Ser 1 5 (2) INFORMATION FOR SEQ ID NO:26: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 23 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: RNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26: GCGCCCCCUG GGAUACCAGG AUC 23 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:27: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 17 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27: TCAGCAGGTG GCATAGG 17 (2) INFORMATION FOR SEQ ID NO:28: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 32 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 6..32 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28: CCCAT ATG GGC GAT GCC GGC GTC GCG TCA CAG 32 Met Gly Asp Ala Gly Val Ala Ser Gin 1 5 (2) INFORMATION FOR SEQ ID NO:29: (i) SEOUENCE CHARACTERISTICS: (A) LENGTH: 9 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein - N-terminal (xi) SEQUENCE DESCRIPTION: SEQ ID N0:29: Met Gly Asp Ala Gly Val Ala Ser Gin SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:30: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 32 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 6..32 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30: CCCAT ATG AGC GAG GCC GGC GTC GCG TCA CAG 32 Met Ser Glu Ala Gly Val Ala Ser Gin 1 5 (2) INFORMATION FOR SEQ ID NO:31: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 9 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein - N-terminal (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31: Met Ser Glu Ala Gly Val Ala Ser Gin 1 5 (2) INFORMATION FOR SEQ ID NO:32: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 1..27 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32: ATG GAG GAT GCT GGA GTG GCG TCA CAG 27 Met Glu Asp Ala Gly Val Ala Ser Gin 1 5 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:33: (i) SEOUENCE CHARACTERISTICS: (A) LENGTH: 9 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein - N-terminal (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33: Met Glu Asp Ala Gly Val Ala Ser Gin 1 5 (2) INFORMATION FOR SEQ ID NO:34: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34: GGGGGATCCC GCGGATTTAT GAGCGAG 27 (2) INFORMATION FOR SEQ ID NO:35: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 32 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35: GGGGGATCCC GCGGAGACAT GAGCGAGCAC AC 32 (2) INFORMATION FOR SEQ ID NO:36: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36: GGGGGATCCA GCGACATGAG AGATGCTGGA GTGG 34 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:37: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 34 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37: GGGGGATCCA GCGACATGAG AGATGCTGGA GTGG 34 (2) INFORMATION FOR SEQ ID NO:38: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 26 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38: GGGGGATCCG TTCTGCCTCC CCGGAC 26 (2) INFORMATION FOR SEQ ID NO:39: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5312 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 37..5145 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39: GTTCTGCCTC CCCCGGACGG TAAATATAGG GGAACA ATG TAC GCG AAA GCG ACA 54 Met Tyr Ala Lys Ala Thr 1 5 GAC GTG GCG CGT GTC TAC GCC GCG GCA GAT GTC GCC TAC GCG AAC GTA 102 Asp Val Ala Arg Val Tyr Ala Ala Ala Asp Val Ala Tyr Ala Asn Val 10 15 20 CTG CAG CAG AGA GCA GTC AAG TTG GAC TTC GCC CCG CCA CTG AAG GCA 150 Leu Gin Gin Arg Ala Val Lys Leu Asp Phe Ala Pro Pro Leu Lys Ala 25 30 35 CTA GAA ACC CTC CAC AGA CTG TAC TAT CCG CTG CGC TTC AAA GGG GGC 198 Leu Glu Thr Leu His Arg Leu Tyr Tyr Pro Leu Arg Phe Lys Gly Gly 40 45 50 SUBSTITUTE SHEET 88 CAA CGT GTC 246 ACT TTA CCC CCG ACA CAA CAC CCG ATC CTG GCC GGG CAC Pro Pro Gin Arg Val Thr Leu Thr Gin His Pro He Leu Ala Gly His 70 55 60 65 AAT TTC GCC AGG GGA CGT AGC ACA GTG CTC 294 GCA GAA GAG GTT CTG CAC Thr Val Leu Ala Glu Glu Val Leu His Asn Phe Ala Arg Gly Arg Ser 75 80 85 CTG CAC AGC GCA CTT AAG CTA CAT GGG GCA CCG 342 GAG ATA GGG CCG TCT Glu He Gly Pro Ser Leu His Ser Ala Leu Lys Leu His Gly Ala Pro 90 95 100 390 AAC GCC CCC GTC GCA GAC TAT CAC GGG TGC ACC AAG TAC GGC ACC CGC Asn Ala Pro Val Ala Asp Tyr His Gly Cys Thr Lys Tyr Gly Thr Arg 105 110 115 TA GAG TCT AGA TCC GTC GCC ACA 438 GAC GGC TCG CGA CAC ATT ACG GCC T Asp Gly Ser Arg His He Thr Ala Leu Glu Ser Arg Ser Val Ala Thr 120 125 130 CC GAG TTC AAG GCC GAC GCC TCA CTG CTC GC 486 GGC CGG C C AAC GGC ATT Gly Arg Pro Glu Phe Lys Ala Asp Ala Ser Leu Leu Ala Asn Gly He 135 140 145 150 534 GCC TCC CGC ACC TTC TGC GTC GAC GGA GTC GGC TCT TGC GCG TTC AAA Ala Ser Arg Thr Phe Cys Val Asp Gly Val Gly Ser Cys Ala Phe Lys 155 160 165 C TAT GAC GTG ACC CTA GAG 582 TCG CGC GTT GGA ATT GCC AAT CAC TCC CT Ser Arg Val Gly He Ala Asn His Ser Leu Tyr Asp Val Thr Leu Glu 170 175 180 GAG CTG GCC AAT GCG TTT GAG AAC CAC GGA CTT CAC ATG GTC CGC GCG 630 Glu Leu Ala Asn Ala Phe Glu Asn His Gly Leu His Met Val Arg Ala 185 190 195 GTT AAT 678 TTC ATG CAC ATG CCA GAA GAG CTG CTC TAC ATG GAC AAC GTG Asn Phe Met His Met Pro Glu Glu Leu Leu Tyr Met Asp Asn Val Val 200 205 210 GCC GAG CTC GGC TAC CGC TTC CAC GTT ATT GAA GAG CCT ATG GCT GTG 726 Ala Glu Leu Gly Tyr Arg Phe His Val He Glu Glu Pro Met Ala Val 215 220 225 230 74 AAG GAC TGC GCA TTC CAG GGG GGG GAC CTC CGT CTC CAC TTC CCT GAG 7 Lys Asp Cys Ala Phe Gin Gly Gly Asp Leu Arg Leu His Phe Pro Glu 235 240 245 CC 822 TTG GAC TTC ATC AAC GAG AGC CAA GAG CGG CGC ATC GAG AGG CTG G Leu Asp Phe He Asn Glu Ser Gin Glu Arg Arg He Glu Arg Leu Ala 250 255 260 870 GCC CGC GGC TCC TAC TCC AGA CGC GCC GTC ATT TTC TCC GGC GAC GAC Ala Arg Gly Ser Tyr Ser Arg Arg Ala Val He Phe Ser Gly Asp Asp 265 270 275 GAC TGG GGT GAT GCG TAC TTA CAC GAC TTC CAC ACA TGG CTC GCC TAC 918 Asp Trp Gly Asp Ala Tyr Leu His Asp Phe His Thr Trp Leu Ala Tyr 280 285 290 CTA CTG GTG AGG AAC TAC CCC ACT CCG TTT GGT TTC TCA CTC CAT ATA 966 Leu Leu Val Arg Asn Tyr Pro Thr Pro Phe Gly Phe Ser Leu His He 295 300 305 310 [ SUBSTITUTE SHEET GAA GTC CAG AGG CGC CAC GGC TCC AGC ATT GAG CTG CGC ATC ACT CGC 1014 Glu Val Gin Arg Arg His Gly Ser Ser He Glu Leu Arg He Thr Arg 315 320 325 GCG CCA CCT GGA GAC CGC ATG CTG GCC GTC GTC CCA AGG ACG TCC CAA 1062 Ala Pro Pro Gly Asp Arg Met Leu Ala Val Val Pro Arg Thr Ser Gin 330 335 340 GGC CTC TGC AGA ATC CCA AAC ATC TTT TAT TAC GCC GAC GCG TCG GGC 1110 Gly Leu Cys Arg He Pro Asn He Phe Tyr Tyr Ala Asp Ala Ser Gly 345 350 355 ACT GAG CAT AAG ACC ATC CTT ACG TCA CAG CAC AAA GTC AAC ATG CTG 1158 Thr Glu His Lys Thr He Leu Thr Ser Gin His Lys Val Asn Met Leu 360 365 370 CTC AAT TTT ATG CAA ACG CGT CCT GAG AAG GAA CTA GTC GAC ATG ACC 1206 Leu Asn Phe Met Gin Thr Arg Pro Glu Lys Glu Leu Val Asp Met Thr 375 380 385 390 GTC TTG ATG TCG TTC GCG CGC GCT AGG CTG CGC GCG ATC GTG GTC GCC 1254 Val Leu Met Ser Phe Ala Arg Ala Arg Leu Arg Ala He Val Val Ala 395 400 405 TCA GAA GTC ACC GAG AGC TCC TGG AAC ATC TCA CCG GCT GAC CTG GTC 1302 Ser Glu Val Thr Glu Ser Ser Trp Asn He Ser Pro Ala Asp Leu Val 410 415 420 CGC ACT GTC GTG TCT CTT TAC GTC CTC CAC ATC ATC GAG CGC CGA AGG 1350 Arg Thr Val Val Ser Leu Tyr Val Leu His He He Glu Arg Arg Arg 425 430 435 GCT GCG GTC GCT GTC AAG ACC GCC AAG GAC GAC GTC TTT GGA GAG ACT 1398 Ala Ala Val Ala Val Lys Thr Ala Lys Asp Asp Val Phe Gly Glu Thr 440 445 450 TCG TTC TGG GAG AGT CTC AAG CAC GTC TTG GGC TCC TGT TGC GGT CTG 1446 Ser Phe Trp Glu Ser Leu Lys His Val Leu Gly Ser Cys Cys Gly Leu 455 460 465 470 CGC AAC CTC AAA GGC ACC GAC GTC GTC TTT ACT AAG CGC GTC GTC GAT 1494 Arg Asn Leu Lys Gly Thr Asp Val Val Phe Thr Lys Arg Val Val Asp 475 480 485 AAG TAC CGA GTC CAC TCG CTC GGA GAC ATA ATC TGC GAC GTC CGC CTG 1542 Lys Tyr Arg Val His Ser Leu Gly Asp He He Cys Asp Val Arg Leu 490 495 500 TCC CCT GAA CAG GTC GGC TTC CTG CCG TCC CGC GTA CCA CCT GCC CGC 1590 Ser Pro Glu Gin Val Gly Phe Leu Pro Ser Arg Val Pro Pro Ala Are 505 510 515 GTC TTT CAC GAC AGG GAA GAG CTT GAG GTC CTT CGC GAA GCT GGC TGC 1638 Val Phe His Asp Arg Glu Glu Leu Glu Val Leu Arg Glu Ala Gly Cys 520 525 530 TAC AAC GAA CGT CCG GTA CCT TCC ACT CCT CCT GTG GAG GAG CCC CAA 1686 Tyr Asn Glu Arg Pro Val Pro Ser Thr Pro Pro Val Glu Glu Pro Gin 535 540 545 550 SUBSTITUTE SHEET GGT TTC GAC GCC GAC TTG TGG CAC GCG ACC GCG GCC TCA CTC CCC GAG 1734 Gly Phe Asp Ala Asp Leu Trp His Ala Thr Ala Ala Ser Leu Pro Glu 555 560 565 TAC CGC GCC ACC TTG CAG GCA GGT CTC AAC ACC GAC GTC AAG CAG CTC 1782 Tyr Arg Ala Thr Leu Gin Ala Gly Leu Asn Thr Asp Val Lys Gin Leu 570 575 580 AAG ATC ACC CTC GAG AAC GCC CTC AAG ACC ATC GAC GGG CTC ACC CTC 1830 Lys He Thr Leu Glu Asn Ala Leu Lys Thr He Asp Gly Leu Thr Leu 585 590 595 TCC CCA GTC AGA GGC CTC GAG ATG TAC GAG GGC Ser Pro CCG CCA GGC AGC GGC 1878 Val Arg Gly Leu Glu Met Tyr Glu Gly Pro Pro Gly Ser Gly 600 605 610 AAG ACG GGC ACC CTC ATC GCC GCC CTT GAG GCC Lys Thr GCG GGC GGT AAA GCA 1926 Gly Thr Leu He Ala Ala Leu Glu Ala Ala Gly Gly Lys Ala 615 620 625 630 CTT TAC GTG GCA CCC ACC AGA GAA CTG AGA GAG Leu Tyr GCT ATG GAC CGG CGG 1974 Val Ala Pro Thr Arg Glu Leu Arg Glu Ala Met Asp Arg Arg 635 640 645 ATC AAA CCG CCG TCC GCC TCG GCT ACG CAA CAT He Lys GTC GCC CTT GCG ATT 2022 Pro Pro Ser Ala Ser Ala Thr Gin His Val Ala Leu Ala He 650 655 660 CTC CGT CGT GCC ACC GCC GAG GGC GCC CCT TTC Leu Arg GCT ACC GTG GTT ATC 2070 Arg Ala Thr Ala Glu Gly Ala Pro Phe Ala Thr Val Val He 665 670 675 GAC GAG TGC TTC ATG TTC CCG CTC GTG TAC GTC Asp Glu GCG ATC GTG CAC GCC 2118 Cys Phe Met Phe Pro Leu Val Tyr Val Ala He Val His Ala 680 685 690 TTG TCC CCG AGC TCA CGA ATA GTC CTT GTA GGG Leu Ser GAC GTC CAC CAA ATC 2166 Pro Ser Ser Arg He Val Leu Val Gly Asp Val His Gin He 695 700 705 710 GGG TTT ATA GAC TTC CAA GGC ACA AGC GCG AAC Gly Phe ATG CCG CTC GTT CGC 2214 He Asp Phe Gin Gly Thr Ser Ala Asn Met Pro Leu Val Arg 715 720 725 GAC GTC GTT AAG CAG TGC CGT CGG CGC ACT TTC Asp Val AAC CAA ACC AAG CGC 2262 Val Lys Gin Cys Arg Arg Arg Thr Phe Asn Gin Thr Lys Arg 730 735 740 TGT CCG GCC GAC GTC GTT GCC ACC ACG TTT TTC Cys Pro CAG AGC TTG TAC CCC 2310 Ala Asp Val Val Ala Thr Thr Phe Phe Gin Ser Leu Tyr Pro 745 750 755 GGG TGC ACA ACC ACC TCA GGG TGC GTC GCA TCC Gly Cys ATC AGC CAC GTC GCC 2358 Thr Thr Thr Ser Gly Cys Val Ala Ser He Ser His Val Ala 760 765 770 CCA GAC TAC CGC AAC AGC CAG GCG CAA ACG CTC TGC TTC ACG CAG GAG 2406 Pro Asp Tyr Arg Asn Ser Gin Ala Gin Thr Leu Cys Phe Thr Gin Glu 775 780 785 790 SUBSTITUTE SHEET GAA AAG TCG CGC CAC GGG GCT GAG GGC GCG ATG ACT GTG CAC GAA GCG 2454 Glu Lys Ser Arg His Gly Ala Glu Gly Ala Met Thr Val His Glu Ala 795 800 805 CAG GGA CGC ACT TTT GCG TCT GTC ATT CTG CAT TAC AAC GGC TCC ACA 2502 Gin Gly Arg Thr Phe Ala Ser Val He Leu His Tyr Asn Gly Ser Thr 810 815 820 GCA GAG CAG AAG CTC CTC GCT GAG AAG TCG CAC CTT CTA GTC GGC ATC 2550 Ala Glu Gin Lys Leu Leu Ala Glu Lys Ser His Leu Leu Val Gly He 825 830 835 ACG CGC CAC ACC AAC CAC CTG TAC ATC CGC GAC CCG ACA GGT GAC ATT 2598 Thr Arg His Thr Asn His Leu Tyr He Arg Asp Pro Thr Gly Asp He 840 845 850 GAG AGA CAA CTC AAC CAT AGC GCG AAA GCC GAG GTG TTT ACA GAC ATC 2646 Glu Arg Gin Leu Asn His Ser Ala Lys Ala Glu Val Phe Thr Asp He 855 860 865 870 CCT GCA CCC CTG GAG ATC ACG ACT GTC AAA CCG AGT GAA GAG GTG CAG 2694 Pro Ala Pro Leu Glu He Thr Thr Val Lys Pro Ser Glu Glu Val Gin 875 880 885 CGC AAC GAA GTG ATG GCA ACG ATA CCC CCG CAG AGT GCC ACG CCG CAC 2742 Arg Asn Glu Val Met Ala Thr He Pro Pro Gin Ser Ala Thr Pro His 890 895 900 GGA GCA ATC CAT CTG CTC CGC AAG AAC TTC GGG GAC CAA CCC GAC TGT 2790 Gly Ala He His Leu Leu Arg Lys Asn Phe Gly Asp Gin Pro Asp Cys 905 910 915 GGC TGT GTC GCT TTG GCG AAG ACC GGC TAC GAG GTG TTT GGC GGT CGT 2838 Gly Cys Val Ala Leu Ala Lys Thr Gly Tyr Glu Val Phe Gly Gly Arg 920 925 930 GCC AAA ATC AAC GTA GAG CTT GCC GAA CCC GAC GCG ACC CCG AAG CCG 2886 Ala Lys He Asn Val Glu Leu Ala Glu Pro Asp Ala Thr Pro Lys Pro 935 940 945 950 CAT AGG GCG TTC CAG GAA GGG GTA CAG TGG GTC AAG GTC ACC AAC GCG 2934 His Arg Ala Phe Gin Glu Gly Val Gin Trp Val Lys Val Thr Asn Ala 955 960 965 TCT AAC AAA CAC CAG GCG CTC CAG ACG CTG TTG TCC CGC TAC ACC AAG 2982 Ser Asn Lys His Gin Ala Leu Gin Thr Leu Leu Ser Arg Tyr Thr Lys 970 975 980 CGA AGC GCT GAC CTG CCG CTA CAC GAA GCT AAG GAG GAC GTC AAA CGC 3030 Arg Ser Ala Asp Leu Pro Leu His Glu Ala Lys Glu Asp Val Lys Arg 985 990 995 ATG CTA AAC TCG CTT GAC CGA CAT TGG GAC TGG ACT GTC ACT GAA GAC 3078 Met Leu Asn Ser Leu Asp Arg His Trp Asp Trp Thr Val Thr Glu Asp 1000 1005 1010 GCC CGT GAC CGA GCT GTC TTC GAG ACC CAG CTC AAG TTC ACC CAA CGC 3126 Ala Arg Asp Arg Ala Val Phe Glu Thr Gin Leu Lys Phe Thr Gin Arg 1015 1020 1025 1030 SUBSTITUTE SHEET GGC GGC ACC GTC GAA GAC CTG CTG GAG CCA GAC GAC CCC TAC ATC CGT 3174 Gly Gly Thr Val Glu Asp Leu Leu Glu Pro Asp Asp Pro Tyr He Are 1035 1040 1045 GAC ATA GAC TTC CTT ATG AAG ACT CAG CAG AAA GTG TCG CCC AAG CCG 3222 Asp He Asp Phe Leu Met Lys Thr Gin Gin Lys Val Ser Pro Lys Pro 1050 1055 1060 ATC AAT ACG GGC AAG GTC GGG CAG GGG ATC GCC GCT CAC TCA AAG TCT 3270 He Asn Thr Gly Lys Val Gly Gin Gly He Ala Ala His Ser Lys Ser 1065 1070 1075 CTC AAC TTC GTC CTC GCC GCT TGG ATA CGC ATA CTC GAG GAG ATA CTC 3318 Leu Asn Phe Val Leu Ala Ala Trp He Arg He Leu Glu Glu He Leu 1080 1085 1090 CGT ACC GGG AGC CGC ACG GTC CGG TAC AGC AAC GGT CTC CCC GAC GAA 3366 Arg Thr Gly Ser Arg Thr Val Arg Tyr Ser Asn Gly Leu Pro Asp Glu 1095 1100 H05 1110 GAA GAG GCC ATG CTG CTC GAA GCG AAG ATC AAT CAA GTC CCA CAC GCC 3414 Glu Glu Ala Met Leu Leu Glu Ala Lys He Asn Gin Val Pro His Ala 1115 1120 H25 ACG TTC GTC TCG GCG GAC TGG ACC GAG TTT GAC ACC GCC CAC AAT AAC 3462 Thr Phe Val Ser Ala Asp Trp Thr Glu Phe Asp Thr Ala His Asn Asn 1130 1135 1140 ACG AGT GAG CTG CTC TTC GCC GCC CTT TTA GAG CGC ATC GGC ACG CCT 3510 Thr Ser Glu Leu Leu Phe Ala Ala Leu Leu Glu Arg He Gly Thr Pro 1145 H50 1155 GCA GCT GCC GTT AAT CTA TTC AGA GAA CGG TGT GGG AAA CGC ACC TTG 3558 Ala Ala Ala Val Asn Leu Phe Arg Glu Arg Cys Gly Lys Arg Thr Leu Ho0 1165 1170 CGA GCG AAG GGT CTA GGC TCC GTT GAA GTC GAC GGT CTG CTC GAC TCC 3606 Arg Ala Lys Gly Leu Gly Ser Val Glu Val Asp Gly Leu Leu Asp Ser 1175 H80 1185 1190 GGC GCA GCT TGG ACG CCT TGC CGC AAC ACC ATC TTC TCT GCC GCC GTC 3654 Gly Ala Ala Trp Thr Pro Cys Arg Asn Thr He Phe Ser Ala Ala Val 1195 1200 1205 ATG CTC ACG CTC TTC CGC GGC GTC AAG TTC GCA GCT TTC AAA GGC GAC 3702 Met Leu Thr Leu Phe Arg Gly Val Lys Phe Ala Ala Phe Lys Gly Asp 1210 1215 1220 GAC TCG CTC CTC TGT GGT AGC CAT TAC CTC CGT TTC GAC GCT AGC CGC 3750 Asp Ser Leu Leu Cys Gly Ser His Tyr Leu Arg Phe Asp Ala Ser Are 1225 1230 1235 CTT CAC ATG GGC GAA CGT TAC AAG ACC AAA CAT TTG AAG GTC GAG GTG 3798 Leu H i s Met Gly Glu Arg Tyr Lys Thr Lys His Leu Lys Val Glu Val 1240 1245 1250 CAG AAA ATC GTG CCG TAC ATC GGA CTC CTC GTC TCC GCT GAG CAG GTC 3846 ,i Lys Ile Val Pro τ y r Ile G1 Y Leu Le Val Ser Ala Glu Gin Val 1255 1260 1265 1270 SUBSTITUTE SHEET GTC CTC GAC CCT GTC AGG AGC GCT CTC AAG ATA TTT GGG CGC TGC TAC Val Leu Asp Pro Val Arg Ser Ala Leu Lys He Phe Gly Arg Cys T y r 3894 127 5 1280 1285 ACA AGC GAA CTC CTT TAC TCC AAG TAC GTG GAG GCT GTG AG A GAC ATC Thr Ser Glu Leu Leu Tyr Ser Lys Tyr Val Glu 5Ϊ ϊaϊ £ ϊsp He 3942 A yu 1295 1300 ACC AAG GGC TGG AGT GAC GCC CGC TAC CAC AGC CTC CTG TGC CAC ATG Thr Lys Gly Trp Ser Asp Ala Arg Tyr His Ser Leu Leu 6ys His KS. 3990 1 - 3U - > 1310 1315 TCA TAC TAC AAT CCG GAG TCT GCG GCG TAC ATC ATC 4038 Tyr Tyr ASn Pro Glu Ser A 13 la 30Al* Tyr ϊle ϊle GAC GCT GTT GTT CGC TTT GGG CGC GGC GAC TTC CCG TTT GAA CAA CTG Asp Ala Val Val Arg Phe Gly Arg Gly Asp Phe Pro ΪΞ GlS Sit £eu 4086 1ΛΛ: > 1340 1345 1350 CGC GTG GTG CGT GCC CAT GTG CAG GCA CCC GAC GCT TAC AΓΓ t rr rr Arg Val Val Arg Ala His Val Gin Ala Pro Asp Sϊ ϊyr ter ter T^r 4134 135 5 1360 1365 TAT CCG GCT AAC GTG CGC GCA TCG TGC CTT GAC CAC GT C TT r ΓHΓ rrr Tyr Pro Ala Asn Val Arg Ala Ser Cys ill ^p His vll III tl_ g* 4182 1J/U 1375 1380 S A rge GGiinn SAlSa AAllϊa A5?la C SP C r G o A??l A a Gl T y ^Phe V GT a T l A GC l G a A Th CA r C T y GT s AGClGa ALA y Gs PCCrGo 4230 ■ " •JOJ 1390 1395 GAA ACG CCT TCT TCA CTT ACC GCG AAA GCT GGT GTT TCT GCG ACT ACA 4278 Ϊ 1 4 H 5 U θ U ° Sβr Sβr LβU Ϊ1Ϊ4!05 A LyS Ala G1 ? V14a1l0 III Ala τ5 Thr AGC CAC GTT GCG ACT GGG ACT GCG CCC CCG GAG TCT C C A ΥCC Γ ΔT ΓΓA Ser H i s Val Ala Thr Gly Thr Ala Pro PrS ftS ler Pro r£ ΪJJ Alt 4326 A ^ u 1425 1 30 PPrrϊo AAllta AA?lSa Asn Se C r IPIh 1 e IS C e G r ^Glu G T L T e A u T L T eu G A Th CA r P CC r G o G G A lu G AThCCr PCCrGo TSCeCr 4374 1435 140 14 45 ACA TCA TCC TCG CCG TCA TCG TCT TCA TCG GAC TCC TCT ACA TCG TGT Thr Ser Ser Ser Pro Ser Ser Ser Ser Ser Asp Ser Ser Thr Ser Cys 4422 1 ^- )U 1455 1 60 fGilty ϊArϊg 2Se£r ?L T eu C Se T r G?l T y 2G?l A y A A sp C A Th CC r A GC l A a A AG r G g A Th CC r A T G hr GGAlAu AGAspC TLTeuG 4470 lilb -> 1470 1475 U t? A GA ^ G CG S CT TCG CAA GAC AGG CAA TCA CGC TCG TCT GAA 4518 1 i 4 tt 8 o 0 u g 7S Pr ° ?14?8p5, Gln ASP Arg Gln S 14 er 90 Ar 8 Ser Ser Git TGT CTG GAC AGA AGC GGA GAA AGG ACA GGC AGT TCG TTA ACT GCC CCC Cys Leu Asp Arg Ser Gly Glu Arg Thr Gly Ser £er St Thϊ Ma Pro 4566 i y:> 15 00 1505 i5io SUBSTITUTE SHEET PCI7AU93/00411 94 E E ^ J E E 55 E E 1550 ig E E 215555 E E £ ™ E E £ E g j£E E 55 E S E E E 5 E "• ss SΪ 3 ° 33 at ss g lfi 00 as 1605 -» E 5555 E E SS E 1615 g E E £ 1620 E E < 902 E E E iJ g E E E E 1630 E fit E E 1635 E E E * » • E SS E E S S B E E E jg E E g E * 998 §| E 55 E ffi S g E E E E S E S E 5 °" E E E E S Ξ E E E S E E E E Ξ E 5094 AGC GCA TGAAGGAACA AAATTAGTTT CCTTGTTCGT AAACAAGGTG GTCCCTCCCA 5198 TTGAGGTAAA GACTCTGGTG AGTCCTCAAC GTTACTCGTT GAGTCTGCTG CGGTTCGATT 5258 CCATTCCCAA GCAGCAAAGG GTGCGCAACT AGTACGGCGC CCCCTGGGAT ACCA 5312 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:40: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1703 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40: Met Tyr Ala Lys Ala Thr Asp Val Ala Arg Val Tyr Ala Ala Ala Asp 1 5 10 15 Val Ala Tyr Ala Asn Val Leu Gin Gin Arg Ala Val Lys Leu Asp Phe 20 25 30 Ala Pro Pro Leu Lys Ala Leu Glu Thr Leu His Arg Leu Tyr Tyr Pro 35 40 45 Leu Arg Phe Lys Gly Gly Thr Leu Pro Pro Thr Gin His Pro He Leu 50 55 60 Ala Gly His Gin Arg Val Ala Glu Glu Val Leu His Asn Phe Ala Arg 65 70 75 80 Gly Arg Ser Thr Val Leu Glu He Gly Pro Ser Leu His Ser Ala Leu 85 90 95 Lys Leu His Gly Ala Pro Asn Ala Pro Val Ala Asp Tyr His Gly Cys 100 105 110 Thr Lys Tyr Gly Thr Arg Asp Gly Ser Arg His He Thr Ala Leu Glu 115 120 125 Ser Arg Ser Val Ala Thr Gly Arg Pro Glu Phe Lys Ala Asp Ala Ser 130 135 140 Leu Leu Ala Asn Gly He Ala Ser Arg Thr Phe Cys Val Asp Gly Val 145 150 155 160 Gly Ser Cys Ala Phe Lys Ser Arg Val Gly He Ala Asn His Ser Leu 165 170 175 Tyr Asp Val Thr Leu Glu Glu Leu Ala Asn Ala Phe Glu Asn His Gly 180 185 190 Leu His Met Val Arg Ala Phe Met His Met Pro Glu Glu Leu Leu Tyr 195 200 205 Met Asp Asn Val Val Asn Ala Glu Leu Gly Tyr Arg Phe His Val He 210 215 220 Glu Glu Pro Met Ala Val Lys Asp Cys Ala Phe Gin Gly Gly Asp Leu 225 230 235 240 Arg Leu His Phe Pro Glu Leu Asp Phe He Asn Glu Ser Gin Glu Arg 245 250 255 Arg He Glu Arg Leu Ala Ala Arg Gly Ser Tyr Ser Arg Arg Ala Val 260 265 270 SUBSTITUTE SHEET He Phe Ser Gly Asp Asp Asp Trp Gly Asp Ala Tyr Leu His Asp Phe 275 280 285 His Thr Trp Leu Ala Tyr Leu Leu Val Arg Asn Tyr Pro Thr Pro Phe 290 295 300 Gly Phe Ser Leu His He Glu Val Gin Arg Arg His Gly Ser Ser He 305 310 315 320 Glu Leu Arg He Thr Arg Ala Pro Pro Gly Asp Arg Met Leu Ala Val 325 330 335 Val Pro Arg Thr Ser Gin Gly Leu Cys Arg He Pro Asn He Phe Tyr 340 345 350 Tyr Ala Asp Ala Ser Gly Thr Glu His Lys Thr He Leu Thr Ser Gin 355 360 365 His Lys Val Asn Met Leu Leu Asn Phe Met Gin Thr Arg Pro Glu Lvs 370 375 380 Glu Leu Val Asp Met Thr Val Leu Met Ser Phe Ala Arg Ala Arg Leu 385 390 395 400 Arg Ala He Val Val Ala Ser Glu Val Thr Glu Ser Ser Trp Asn He 405 410 415 Ser Pro Ala Asp Leu Val Arg Thr Val Val Ser Leu Tyr Val Leu His 420 425 430 He He Glu Arg Arg Arg Ala Ala Val Ala Val Lys Thr Ala Lys Asp 435 440 445 Asp Val Phe Gly Glu Thr Ser Phe Trp Glu Ser Leu Lys His Val Leu 450 455 460 Gly Ser Cys Cys Gly Leu Arg Asn Leu Lys Gly Thr Asp Val Val Phe 465 470 475 480 Thr Lys Arg Val Val Asp Lys Tyr Arg Val His Ser Leu Gly Asp He 485 490 495 He Cys Asp Val Arg Leu Ser Pro Glu Gin Val Gly Phe Leu Pro Ser 500 505 510 Arg Val Pro Pro Ala Arg Val Phe His Asp Arg Glu Glu Leu Glu Val 515 520 525 Leu Arg Glu Ala Gly Cys Tyr Asn Glu Arg Pro Val Pro Ser Thr Pro 530 535 540 Pro Val Glu Glu Pro Gin Gly Phe Asp Ala Asp Leu Trp His Ala Thr 545 550 555 560 Ala Ala Ser Leu Pro Glu Tyr Arg Ala Thr Leu Gin Ala Gly Leu Asn 565 570 575 Thr Asp Val Lys Gin Leu Lys He Thr Leu Glu Asn Ala Leu Lys Thr 580 585 590 j SUBSTITUTE SHEET 9.7 He Asp Gly Leu Thr Leu Ser Pro Val Arg Gly Leu Glu Met Tyr Glu 595 600 605 Gly Pro Pro Gly Ser Gly Lys Thr Gly Thr Leu He Ala Ala Leu Glu 610 615 620 Ala Ala Gly Gly Lys Ala Leu Tyr Val Ala Pro Thr Arg Glu Leu Arg 625 630 635 640 Glu Ala Met Asp Arg Arg He Lys Pro Pro Ser Ala Ser Ala Thr Gin 645 650 655 His Val Ala Leu Ala He Leu Arg Arg Ala Thr Ala Glu Gly Ala Pro 660 665 670 Phe Ala Thr Val Val He Asp Glu Cys Phe Met Phe Pro Leu Val Tyr 675 680 685 Val Ala He Val His Ala Leu Ser Pro Ser Ser Arg He Val Leu Val 690 695 700 Gly Asp Val His Gin He Gly Phe He Asp Phe Gin Gly Thr Ser Ala 705 710 715 720 Asn Met Pro Leu Val Arg Asp Val Val Lys Gin Cys Arg Arg Arg Thr 725 730 735 Phe Asn Gin Thr Lys Arg Cys Pro Ala Asp Val Val Ala Thr Thr Phe 740 745 750 Phe Gin Ser Leu Tyr Pro Gly Cys Thr Thr Thr Ser Gly Cys Val Ala 755 760 765 Ser He Ser His Val Ala Pro Asp Tyr Arg Asn Ser Gin Ala Gin Thr 770 775 780 Leu Cys Phe Thr Gin Glu Glu Lys Ser Arg His Gly Ala Glu Gly Ala 785 790 795 800 Met Thr Val His Glu Ala Gin Gly Arg Thr Phe Ala Ser Val He Leu 805 810 815 His Tyr Asn Gly Ser Thr Ala Glu Gin Lys Leu Leu Ala Glu Lys Ser 820 825 830 His Leu Leu Val Gly He Thr Arg His Thr Asn His Leu Tyr He Arg 835 840 845 Asp Pro Thr Gly Asp He Glu Arg Gin Leu Asn His Ser Ala Lys Ala 850 855 860 Glu Val Phe Thr Asp He Pro Ala Pro Leu Glu He Thr Thr Val Lys 865 870 875 880 Pro Ser Glu Glu Val Gin Arg Asn Glu Val Met Ala Thr He Pro Pro 885 890 895 Gin Ser Ala Thr Pro His Gly Ala He His Leu Leu Arg Lys Asn Phe 900 905 910 SUBSTITUTE SHEET Gly Asp Gin Pro Asp Cys Gly Cys Val Ala Leu Ala Lys Thr Gl y T y r 91 920 925 Glu Val Phe Gly Gly Arg Ala Lys He Asn Val Glu Leu Ala Glu Pro yJU 935 940 Asp Ala Thr Pro Lys Pro His Arg Ala Phe Gin Glu Gly Val Gin Trp 94 950 955 g6 g Val Lys Val Thr Asn Ala Ser Asn Lys His Gin Ala Leu Gin Thr Leu 965 970 9 75 Leu Ser Arg Tyr Thr Lys Arg Ser Ala Asp Leu Pro Leu His Glu Ala 980 985 990 Lys Glu Asp Val Lys Arg Met Leu Asn Ser Leu Asp Arg His Trp Asp 995 1000 1005 Trp ϊ n Val Thr Glu As P Ala Ar S As P Ar δ Ala γ al Phe Glu Thr Gin iUlu 1015 1020 Leu Lys Phe Thr Gin Arg Gly Gly Thr Val Glu Asp Leu Leu Glu Pro 1U 5 0 0 1035 1040 Asp Asp Pro Tyr lie Arg Asp He Asp Phe Leu Met Lys Thr Gin Gin 1045 1050 1055 Lys Val Ser Pro Lys Pro He Asn Thr Gly Lys Val Gly Gin Gly He 1060 1065 1070 Ala Ala His^Ser Lys Ser Leu Asn Phe Val Leu Ala Ala Trp He Arg Ilθ nQn G1U G1U Ilβ eU ^ ε Thr G1 y Ser Ar S Thr Va Arg Tyr Ser iuyu 1095 1100 Asn Gly Leu Pro Asp Glu Glu Glu Ala Met Leu Leu Glu Ala Lys He 111 HI 1115 H20 Asn Gin Val Pro His Ala Thr Phe Val Ser Ala Asp Trp Thr Glu Phe 2 1130 H35 Asp Thr Ala His Asn Asn Thr Ser Glu Leu Leu Phe Ala Ala Leu Leu 1140 H 5 1150 Glu Arg He Gly Thr Pro Ala Ala Ala Val Asn Leu Phe Arg Glu Arg i i > -> 1160 H65 Cys Gly Lys Arg Thr Leu Arg Ala Lys Gly Leu Gly Ser Val Glu Val ii υ 1175 1180 Asp Gly Leu Leu Asp Ser Gly Ala Ala Trp Thr Pro Cys Arg Asn Thr 1185 119 0 1195 6 1200 He Phe Ser Ala Ala Val Met Leu Thr Leu Phe Arg Gly Val L y s Phe 1205 1210 1215 Ala Ala Phe Lys Gly Asp Asp Ser Leu Leu Cys Gly Ser His Tyr Leu 1220 1225 1230 I SUBSTITUTE SHEET Arg Phe Asp Ala Ser Arg Leu His Met Gly Glu Arg Tyr Lys Thr Lys 1235 1240 1245 His Leu Lys Val Glu Val Gin Lys He Val Pro Tyr He Gly Leu Leu 1250 1255 1260 Val Ser Ala Glu Gin Val Val Leu Asp Pro Val Arg Ser Ala Leu Lys 1265 1270 1275 1280 He Phe Gly Arg Cys Tyr Thr Ser Glu Leu Leu Tyr Ser Lys Tyr Val 1285 1290 1295 Glu Ala Val Arg Asp He Thr Lys Gly Trp Ser Asp Ala Arg Tyr His 1300 1305 1310 Ser Leu Leu Cys His Met Ser Ala Cys Tyr Tyr Asn Tyr Ala Pro Glu 1315 1320 1325 Ser Ala Ala Tyr He He Asp Ala Val Val Arg Phe Gly Arg Gly Asp 1330 1335 1340 Phe Pro Phe Glu Gin Leu Arg Val Val Arg Ala His Val Gin Ala Pro 1345 1350 1355 1360 Asp Ala Tyr Ser Ser Thr Tyr Pro Ala Asn Val Arg Ala Ser Cys Leu 1365 1370 1375 Asp His Val Phe Glu Pro Arg Gin Ala Ala Ala Pro Ala Gly Phe Val 1380 1385 1390 Ala Thr Cys Ala Lys Pro Glu Thr Pro Ser Ser Leu Thr Ala Lys Ala 1395 1400 1405 Gly Val Ser Ala Thr Thr Ser His Val Ala Thr Gly Thr Ala Pro Pro 1410 1415 1420 Glu Ser Pro Trp Asp Ala Pro Ala Ala Asn Ser Phe Ser Glu Leu Leu 1425 1430 1435 1440 Thr Pro Glu Thr Pro Ser Thr Ser Ser Ser Pro Ser Ser Ser Ser Ser 1445 1450 1455 Asp Ser Ser Thr Ser Cys Gly Arg Ser Leu Ser Gly Gly Asp Thr Ala 1460 1465 1470 Arg Thr Thr Glu Asp Leu Asn Ser Arg Lys Pro Pro Ser Gin Asp Arg 1475 1480 1485 Gin Ser Arg Ser Ser Glu Cys Leu Asp Arg Ser Gly Glu Arg Thr Gly 1490 1495 1500 Ser Ser Leu Thr Ala Pro Thr Ala Pro Ser Pro Ser Phe Ser Phe Ser 1505 1510 1515 1520 Glu Arg Ala Arg Leu Ala Thr Gly Pro Thr Val Ala Ala Ala Thr Ser 1525 1530 1535 Pro Ser Ala Thr Pro Ser Cys Ala Thr Asp Gin Val Ala Ala Arg Thr 1540 1545 1550 SUBSTITUTE SHEET Thr Pro Asp Phe Ala Pro Phe Leu Gly Ser Gin Ser Ala Arg Ala Val 1555 1560 1565 Ser Lys Pro Tyr Arg Pro Pro Thr Thr Ala Arg Trp Lys Glu Val Thr 1570 1575 1580 Pro Leu His Ala Trp Lys Gly Val Thr Gly Asp Arg Pro Glu Val Arg 1585 1590 1595 1600 Glu Asp Pro Glu Thr Ala Ala Val Val Gin Ala Leu He Ser Gly Arg 1605 1610 1615 Tyr Pro Gin Lys Thr Lys Leu Ser Ser Asp Ala Ser Lys Gly Tyr Ser 1620 1625 1630 Arg Thr Lys Gly Cys Ser Gin Ser Thr Ser Phe Pro Ala Pro Ser Ala 1635 1640 1645 Asp Tyr Gin Ala Arg Asp Cys Gin Thr Val Arg Val Cys Arg Ala Ala 1650 1655 1660 Ala Glu Met Ala Arg Ser Cys He His Glu Pro Leu Ala Ser Ser Ala 1665 1670 1675 1680 Ala Ser Ala Asp Leu Lys Arg He Arg Ser Thr Ser Asp Ser Val Pro 1685 1690 1695 Asp Val Lys He Ser Lys Ser Ala 1700 (2) INFORMATION FOR SEQ ID NO:41: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5312 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 4218..4512 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41: GTTCTGCCTC CCCCGGACGG TAAATATAGG GGAACAATGT ACGCGAAAGC GACAGACGTG 60 GCGCGTGTCT ACGCCGCGGC AGATGTCGCC TACGCGAACG TACTGCAGCA GAGAGCAGTC 120 AAGTTGGACT TCGCCCCGCC ACTGAAGGCA CTAGAAACCC TCCACAGACT GTACTATCCG 180 CTGCGCTTCA AAGGGGGCAC TTTACCCCCG ACACAACACC CGATCCTGGC CGGGCACCAA 240 CGTGTCGCAG AAGAGGTTCT GCACAATTTC GCCAGGGGAC GTAGCACAGT GCTCGAGATA 300 GGGCCGTCTC TGCACAGCGC ACTTAAGCTA CATGGGGCAC CGAACGCCCC CGTCGCAGAC 360 SUBSTITUTE SHEET TATCACGGGT GCACCAAGTA CGGCACCCGC GACGGCTCGC GACACATTAC GGCCTTAGAG 420 TCTAGATCCG TCGCCACAGG CCGGCCCGAG TTCAAGGCCG ACGCCTCACT GCTCGCCAAC 480 GGCATTGCCT CCCGCACCTT CTGCGTCGAC GGAGTCGGCT CTTGCGCGTT CAAATCGCGC 540 GTTGGAATTG CCAATCACTC CCTCTATGAC GTGACCCTAG AGGAGCTGGC CAATGCGTTT 600 GAGAACCACG GACTTCACAT GGTCCGCGCG TTCATGCACA TGCCAGAAGA GCTGCTCTAC 660 ATGGACAACG TGGTTAATGC CGAGCTCGGC TACCGCTTCC ACGTTATTGA AGAGCCTATG 720 GCTGTGAAGG ACTGCGCATT CCAGGGGGGG GACCTCCGTC TCCACTTCCC TGAGTTGGAC 780 TTCATCAACG AGAGCCAAGA GCGGCGCATC GAGAGGCTGG CCGCCCGCGG CTCCTACTCC 840 AGACGCGCCG TCATTTTCTC CGGCGACGAC GACTGGGGTG ATGCGTACTT ACACGACTTC 900 CACACATGGC TCGCCTACCT ACTGGTGAGG AACTACCCCA CTCCGTTTGG TTTCTCACTC 960 CATATAGAAG TCCAGAGGCG CCACGGCTCC AGCATTGAGC TGCGCATCAC TCGCGCGCCA 1020 CCTGGAGACC GCATGCTGGC CGTCGTCCCA AGGACGTCCC AAGGCCTCTG CAGAATCCCA 1080 AACATCTTTT ATTACGCCGA CGCGTCGGGC ACTGAGCATA AGACCATCCT TACGTCACAG 1140 CACAAAGTCA ACATGCTGCT CAATTTTATG CAAACGCGTC CTGAGAAGGA ACTAGTCGAC 1200 ATGACCGTCT TGATGTCGTT CGCGCGCGCT AGGCTGCGCG CGATCGTGGT CGCCTCAGAA 1260 GTCACCGAGA GCTCCTGGAA CATCTCACCG GCTGACCTGG TCCGCACTGT CGTGTCTCTT 1320 TACGTCCTCC ACATCATCGA GCGCCGAAGG GCTGCGGTCG CTGTCAAGAC CGCCAAGGAC 1380 GACGTCTTTG GAGAGACTTC GTTCTGGGAG AGTCTCAAGC ACGTCTTGGG CTCCTGTTGC 1440 GGTCTGCGCA ACCTCAAAGG CACCGACGTC GTCTTTACTA AGCGCGTCGT CGATAAGTAC 1500 CGAGTCCACT CGCTCGGAGA CATAATCTGC GACGTCCGCC TGTCCCCTGA ACAGGTCGGC 1560 TTCCTGCCGT CCCGCGTACC ACCTGCCCGC GTCTTTCACG ACAGGGAAGA GCTTGAGGTC 1620 CTTCGCGAAG CTGGCTGCTA CAACGAACGT CCGGTACCTT CCACTCCTCC TGTGGAGGAG 1680 CCCCAAGGTT TCGACGCCGA CTTGTGGCAC GCGACCGCGG CCTCACTCCC CGAGTACCGC 1740 GCCACCTTGC AGGCAGGTCT CAACACCGAC GTCAAGCAGC TCAAGATCAC CCTCGAGAAC 1800 GCCCTCAAGA CCATCGACGG GCTCACCCTC TCCCCAGTCA GAGGCCTCGA GATGTACGAG 1860 GGCCCGCCAG GCAGCGGCAA GACGGGCACC CTCATCGCCG CCCTTGAGGC CGCGGGCGGT 1920 AAAGCACTTT ACGTGGCACC CACCAGAGAA CTGAGAGAGG CTATGGACCG GCGGATCAAA 1980 CCGCCGTCCG CCTCGGCTAC GCAACATGTC GCCCTTGCGA TTCTCCGTCG TGCCACCGCC 2040 GAGGGCGCCC CTTTCGCTAC CGTGGTTATC GACGAGTGCT TCATGTTCCC GCTCGTGTAC 2100 GTCGCGATCG TGCACGCCTT GTCCCCGAGC TCACGAATAG TCCTTGTAGG GGACGTCCAC 2160 j SUBSTITUTE SHEET CAAATCGGGT TTATAGACTT CCAAGGCACA AGCGCGAACA TGCCGCTCGT TCGCGACGTC 2220 GTTAAGCAGT GCCGTCGGCG CACTTTCAAC CAAACCAAGC GCTGTCCGGC CGACGTCGTT 2280 GCCACCACGT TTTTCCAGAG. CTTGTACCCC GGGTGCACAA CCACCTCAGG GTGCGTCGCA 2340 TCCATCAGCC ACGTCGCCCC AGACTACCGC AACAGCCAGG CGCAAACGCT CTGCTTCACG 2400 CAGGAGGAAA AGTCGCGCCA CGGGGCTGAG GGCGCGATGA CTGTGCACGA AGCGCAGGGA 2460 CGCACTTTTG CGTCTGTCAT TCTGCATTAC AACGGCTCCA CAGCAGAGCA GAAGCTCCTC 2520 GCTGAGAAGT CGCACCTTCT AGTCGGCATC ACGCGCCACA CCAACCACCT GTACATCCGC 2580 GACCCGACAG GTGACATTGA GAGACAACTC AACCATAGCG CGAAAGCCGA GGTGTTTACA 2640 GACATCCCTG CACCCCTGGA GATCACGACT GTCAAACCGA GTGAAGAGGT GCAGCGCAAC 2700 GAAGTGATGG CAACGATACC CCCGCAGAGT GCCACGCCGC ACGGAGCAAT CCATCTGCTC 2760 CGCAAGAACT TCGGGGACCA ACCCGACTGT GGCTGTGTCG CTTTGGCGAA GACCGGCTAC 2820 GAGGTGTTTG GCGGTCGTGC CAAAATCAAC GTAGAGCTTG CCGAACCCGA CGCGACCCCG 2880 AAGCCGCATA GGGCGTTCCA GGAAGGGGTA CAGTGGGTCA AGGTCACCAA CGCGTCTAAC 2940 AAACACCAGG CGCTCCAGAC GCTGTTGTCC CGCTACACCA AGCGAAGCGC TGACCTGCCG 3000 CTACACGAAG CTAAGGAGGA CGTCAAACGC ATGCTAAACT CGCTTGACCG ACATTGGGAC 3060 TGGACTGTCA CTGAAGACGC CCGTGACCGA GCTGTCTTCG AGACCCAGCT CAAGTTCACC 3120 CAACGCGGCG GCACCGTCGA AGACCTGCTG GAGCCAGACG ACCCCTACAT CCGTGACATA 3180 GACTTCCTTA TGAAGACTCA GCAGAAAGTG TCGCCCAAGC CGATCAATAC GGGCAAGGTC 3240 GGGCAGGGGA TCGCCGCTCA CTCAAAGTCT CTCAACTTCG TCCTCGCCGC TTGGATACGC 3300 ATACTCGAGG AGATACTCCG TACCGGGAGC CGCACGGTCC GGTACAGCAA CGGTCTCCCC 3360 GACGAAGAAG AGGCCATGCT GCTCGAAGCG AAGATCAATC AAGTCCCACA CGCCACGTTC 3420 GTCTCGGCGG ACTGGACCGA GTTTGACACC GCCCACAATA ACACGAGTGA GCTGCTCTTC 3480 GCCGCCCTTT TAGAGCGCAT CGGCACGCCT GCAGCTGCCG TTAATCTATT CAGAGAACGG 3540 TGTGGGAAAC GCACCTTGCG AGCGAAGGGT CTAGGCTCCG TTGAAGTCGA CGGTCTGCTC 3600 GACTCCGGCG CAGCTTGGAC GCCTTGCCGC AACACCATCT TCTCTGCCGC CGTCATGCTC 3660 ACGCTCTTCC GCGGCGTCAA GTTCGCAGCT TTCAAAGGCG ACGACTCGCT CCTCTGTGGT 3720 AGCCATTACC TCCGTTTCGA CGCTAGCCGC CTTCACATGG GCGAACGTTA CAAGACCAAA 3780 CATTTGAAGG TCGAGGTGCA GAAAATCGTG CCGTACATCG GACTCCTCGT CTCCGCTGAG 3840 CAGGTCGTCC TCGACCCTGT CAGGAGCGCT CTCAAGATAT TTGGGCGCTG CTACACAAGC 3900 GAACTCCTTT ACTCCAAGTA CGTGGAGGCT GTGAGAGACA TCACCAAGGG CTGGAGTGAC 3960 SUBSTITUTE SHEET GCCCGCTACC ACAGCCTCCT GTGCCACATG TCAGCATGCT ACTACAATTA CGCGCCGGAG 4020 TCTGCGGCGT ACATCATCGA CGCTGTTGTT CGCTTTGGGC GCGGCGACTT CCCGTTTGAA 4080 CAACTGCGCG TGGTGCGTGC CCATGTGCAG GCACCCGACG CTTACAGCAG CACGTATCCG 4140 GCTAACGTGC GCGCATCGTG CCTTGACCAC GTCTTCGAGC CCCGCCAGGC CGCCGCCCCG 4200 GCAGGTTTCG TTGCGAC ATG TGC GAA GCC GGA AAC GCC TTC TTC ACT TAC 4250 Met Cys Glu Ala Gly Asn Ala Phe Phe Thr Tyr 1 5 10 CGC GAA AGC TGG TGT TTC TGC GAC TAC AAG CCA CGT TGC GAC TGG GAC 4298 Arg Glu Ser Trp Cys Phe Cys Asp Tyr Lys Pro Arg Cys Asp Trp Asp 15 20 25 TGC GCC CCC GGA GTC TCC ATG GGA TGC ACC TGC AGC CAA CAG CTT TTC 4346 Cys Ala Pro Gly Val Ser Met Gly Cys Thr Cys Ser Gin Gin Leu Phe 30 35 40 GGA GTT ATT GAC ACC GGA GAC CCC GTC CAC ATC ATC CTC GCC GTC ATC 4394 Gly Val He Asp Thr Gly Asp Pro Val His He He Leu Ala Val He 45 50 55 GTC TTC ATC GGA CTC CTC TAC ATC GTG TGG AAG GTC GCT CAG TGG TGG 4442 Val Phe He Gly Leu Leu Tyr He Val Trp Lys Val Ala Gin Trp Trp 60 65 70 75 AGA CAC CGC AAG GAC CAC AGA AGA CTT GAA CAG CAG AAA GCC GCC TTC 4490 Arg His Arg Lys Asp His Arg Arg Leu Glu Gin Gin Lys Ala Ala Phe 80 85 90 GCA AGA CAG GCA ATC ACG CTC GTC TGAATGTC TGGACAGAAG CGGAGAAAGG 4542 Ala Arg Gin Ala He Thr Leu Val 95 ACAGGCAGTT CGTTAACTGC CCCCACTGCT CCGAGCCCCT CATTCTCATT TTCGGAAAGA 4602 GCTCGACTGG CGACCGGGCC GACTGTCGCC GCTGCGACAT CACCTTCGGC AACCCCATCC 4662 TGCGCCACGG ACCAGGTTGC CGCGAGGACC ACGCCGGACT TTGCGCCTTT CCTGGGTTCC 4722 CAGTCTGCCC GTGCTGTCTC GAAGCCGTAC CGGCCCCCCA CGACTGCCCG TTGGAAAGAA 4782 GTCACCCCGC TCCACGCGTG GAAGGGCGTG ACCGGAGACC GACCGGAAGT CAGGGAGGAC 4842 CCGGAGACAG CGGCGGTCGT CCAGGCTCTG ATCAGCGGCC GTTATCCTCA GAAGACGAAG 4902 CTTTCCTCCG ACGCATCCAA AGGCTACTCA AGAACTAAGG GATGCTCACA ATCCACCTCT 4962 TTTCCTGCCC CGAGTGCGGA TTACCAGGCC CGCGACTGCC AGACAGTCCG AGTCTGCCGC 5022 GCCGCTGCAG AGATGGCGCG CTCATGTATT CACGAGCCGT TGGCTTCATC TGCCGCCAGT 5082 GCCGACTTGA AGCGCATACG CTCTACCTCG GACTCTGTTC CCGATGTAAA GATCAGCAAG 5142 AGCGCATGAA GGAACAAAAT TAGTTTCCTT GTTCGTAAAC AAGGTGGTCC CTCCCATTGA 5202 GGTAAAGACT CTGGTGAGTC CTCAACGTTA CTCGTTGAGT CTGCTGCGGT TCGATTCCAT 5262 TCCCAAGCAG CAAAGGGTGC GCAACTAGTA CGGCGCCCCC TGGGATACCA 5312 i SUBSTITUTE SHEET 8 (2) INFORMATION FOR SEQ ID NO:42: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 99 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42: Met Cys Glu Ala Gly Asn Ala Phe Phe Thr Tyr Arg Glu Ser Trp Cys 1 5 10 15 Phe Cys Asp Tyr Lys Pro Arg Cys Asp Trp Asp Cys Ala Pro Gly Val 20 25 30 Ser Met Gly Cys Thr Cys Ser Gin Gin Leu Phe Gly Val He Asp Thr 35 40 45 Gly Asp Pro Val His He He Leu Ala Val He Val Phe He Gly Leu 50 55 60 Leu Tyr He Val Trp Lys Val Ala Gin Trp Trp Arg His Arg Lys Asp 65 70 75 80 His Arg Arg Leu Glu Gin Gin Lys Ala Ala Phe Ala Arg Gin Ala He 85 90 95 Thr Leu Val (2) INFORMATION FOR SEQ ID NO:43: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5312 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 4518..4937 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43: GTTCTGCCTC CCCCGGACGG TAAATATAGG GGAACAATGT ACGCGAAAGC GACAGACGTG 60 GCGCGTGTCT ACGCCGCGGC AGATGTCGCC TACGCGAACG TACTGCAGCA GAGAGCAGTC 120 AAGTTGGACT TCGCCCCGCC ACTGAAGGCA CTAGAAACCC TCCACAGACT GTACTATCCG 180 CTGCGCTTCA AAGGGGGCAC TTTACCCCCG ACACAACACC CGATCCTGGC CGGGCACCAA 240 j SUBSTITUTE SHEET CGTGTCGCAG AAGAGGTTCT GCACAATTTC GCCAGGGGAC GTAGCACAGT GCTCGAGATA 300 GGGCCGTCTC TGCACAGCGC ACTTAAGCTA CATGGGGCAC CGAACGCCCC CGTCGCAGAC 360 TATCACGGGT GCACCAAGTA CGGCACCCGC GACGGCTCGC GACACATTAC GGCCTTAGAG 420 TCTAGATCCG TCGCCACAGG CCGGCCCGAG TTCAAGGCCG ACGCCTCACT GCTCGCCAAC 480 GGCATTGCCT CCCGCACCTT CTGCGTCGAC GGAGTCGGCT CTTGCGCGTT CAAATCGCGC 540 GTTGGAATTG CCAATCACTC CCTCTATGAC GTGACCCTAG AGGAGCTGGC CAATGCGTTT 600 GAGAACCACG GACTTCACAT GGTCCGCGCG TTCATGCACA TGCCAGAAGA GCTGCTCTAC 660 ATGGACAACG TGGTTAATGC CGAGCTCGGC TACCGCTTCC ACGTTATTGA AGAGCCTATG 720 GCTGTGAAGG ACTGCGCATT CCAGGGGGGG GACCTCCGTC TCCACTTCCC TGAGTTGGAC 780 TTCATCAACG AGAGCCAAGA GCGGCGCATC GAGAGGCTGG CCGCCCGCGG CTCCTACTCC 840 AGACGCGCCG TCATTTTCTC CGGCGACGAC GACTGGGGTG ATGCGTACTT ACACGACTTC 900 CACACATGGC TCGCCTACCT ACTGGTGAGG AACTACCCCA CTCCGTTTGG TTTCTCACTC 960 CATATAGAAG TCCAGAGGCG CCACGGCTCC AGCATTGAGC TGCGCATCAC TCGCGCGCCA 1020 CCTGGAGACC GCATGCTGGC CGTCGTCCCA AGGACGTCCC AAGGCCTCTG CAGAATCCCA 1080 AACATCTTTT ATTACGCCGA CGCGTCGGGC ACTGAGCATA AGACCATCCT TACGTCACAG 1140 CACAAAGTCA ACATGCTGCT CAATTTTATG CAAACGCGTC CTGAGAAGGA ACTAGTCGAC 1200 ATGACCGTCT TGATGTCGTT CGCGCGCGCT AGGCTGCGCG CGATCGTGGT CGCCTCAGAA 1260 GTCACCGAGA GCTCCTGGAA CATCTCACCG GCTGACCTGG TCCGCACTGT CGTGTCTCTT 1320 TACGTCCTCC ACATCATCGA GCGCCGAAGG GCTGCGGTCG CTGTCAAGAC CGCCAAGGAC 1380 GACGTCTTTG GAGAGACTTC GTTCTGGGAG AGTCTCAAGC ACGTCTTGGG CTCCTGTTGC 1440 GGTCTGCGCA ACCTCAAAGG CACCGACGTC GTCTTTACTA AGCGCGTCGT CGATAAGTAC 1500 CGAGTCCACT CGCTCGGAGA CATAATCTGC GACGTCCGCC TGTCCCCTGA ACAGGTCGGC 1560 TTCCTGCCGT CCCGCGTACC ACCTGCCCGC GTCTTTCACG ACAGGGAAGA GCTTGAGGTC 1620 CTTCGCGAAG CTGGCTGCTA CAACGAACGT CCGGTACCTT CCACTCCTCC TGTGGAGGAG 1680 CCCCAAGGTT TCGACGCCGA CTTGTGGCAC GCGACCGCGG CCTCACTCCC CGAGTACCGC 1740 GCCACCTTGC AGGCAGGTCT CAACACCGAC GTCAAGCAGC TCAAGATCAC CCTCGAGAAC 1800 GCCCTCAAGA CCATCGACGG GCTCACCCTC TCCCCAGTCA GAGGCCTCGA GATGTACGAG 1860 GGCCCGCCAG GCAGCGGCAA GACGGGCACC CTCATCGCCG CCCTTGAGGC CGCGGGCGGT 1920 AAAGCACTTT ACGTGGCACC CACCAGAGAA CTGAGAGAGG CTATGGACCG GCGGATCAAA 1980 CCGCCGTCCG CCTCGGCTAC GCAACATGTC GCCCTTGCGA TTCTCCGTCG TGCCACCGCC 2040 GAGGGCGCCC CTTTCGCTAC CGTGGTTATC GACGAGTGCT TCATGTTCCC GCTCGTGTAC 2100 GTCGCGATCG TGCACGCCTT GTCCCCGAGC TCACGAATAG TCCTTGTAGG GGACGTCCAC 2160 SUBSTITUTE SHEET CAAATCGGGT TTATAGACTT CCAAGGCACA AGCGCGAACA TGCCGCTCGT TCGCGACGTC 2220 GTTAAGCAGT GCCGTCGGCG CACTTTCAAC CAAACCAAGC GCTGTCCGGC CGACGTCGTT 2280 GCCACCACGT TTTTCCAGAG CTTGTACCCC GGGTGCACAA CCACCTCAGG GTGCGTCGCA 2340 TCCATCAGCC ACGTCGCCCC AGACTACCGC AACAGCCAGG CGCAAACGCT CTGCTTCACG 2400 CAGGAGGAAA AGTCGCGCCA CGGGGCTGAG GGCGCGATGA CTGTGCACGA AGCGCAGGGA 2460 CGCACTTTTG CGTCTGTCAT TCTGCATTAC AACGGCTCCA CAGCAGAGCA GAAGCTCCTC 2520 GCTGAGAAGT CGCACCTTCT AGTCGGCATC ACGCGCCACA CCAACCACCT GTACATCCGC 2580 GACCCGACAG GTGACATTGA GAGACAACTC AACCATAGCG CGAAAGCCGA GGTGTTTACA 2640 GACATCCCTG CACCCCTGGA GATCACGACT GTCAAACCGA GTGAAGAGGT GCAGCGCAAC 2700 GAAGTGATGG CAACGATACC CCCGCAGAGT GCCACGCCGC ACGGAGCAAT CCATCTGCTC 2760 CGCAAGAACT TCGGGGACCA ACCCGACTGT GGCTGTGTCG CTTTGGCGAA GACCGGCTAC 2820 GAGGTGTTTG GCGGTCGTGC CAAAATCAAC GTAGAGCTTG CCGAACCCGA CGCGACCCCG 2880 AAGCCGCATA GGGCGTTCCA GGAAGGGGTA CAGTGGGTCA AGGTCACCAA CGCGTCTAAC 2940 AAACACCAGG CGCTCCAGAC GCTGTTGTCC CGCTACACCA AGCGAAGCGC TGACCTGCCG 3000 CTACACGAAG CTAAGGAGGA CGTCAAACGC ATGCTAAACT CGCTTGACCG ACATTGGGAC 3060 TGGACTGTCA CTGAAGACGC CCGTGACCGA GCTGTCTTCG AGACCCAGCT CAAGTTCACC 3120 CAACGCGGCG GCACCGTCGA AGACCTGCTG GAGCCAGACG ACCCCTACAT CCGTGACATA 3180 GACTTCCTTA TGAAGACTCA GCAGAAAGTG TCGCCCAAGC CGATCAATAC GGGCAAGGTC 3240 GGGCAGGGGA TCGCCGCTCA CTCAAAGTCT CTCAACTTCG TCCTCGCCGC TTGGATACGC 3300 ATACTCGAGG AGATACTCCG TACCGGGAGC CGCACGGTCC GGTACAGCAA CGGTCTCCCC 3360 GACGAAGAAG AGGCCATGCT GCTCGAAGCG AAGATCAATC AAGTCCCACA CGCCACGTTC 3420 GTCTCGGCGG ACTGGACCGA GTTTGACACC GCCCACAATA ACACGAGTGA GCTGCTCTTC 3480 GCCGCCCTTT TAGAGCGCAT CGGCACGCCT GCAGCTGCCG TTAATCTATT CAGAGAACGG 3540 TGTGGGAAAC GCACCTTGCG AGCGAAGGGT CTAGGCTCCG TTGAAGTCGA CGGTCTGCTC 3600 GACTCCGGCG CAGCTTGGAC GCCTTGCCGC AACACCATCT TCTCTGCCGC CGTCATGCTC 3660 ACGCTCTTCC GCGGCGTCAA GTTCGCAGCT TTCAAAGGCG ACGACTCGCT CCTCTGTGGT 3720 AGCCATTACC TCCGTTTCGA CGCTAGCCGC CTTCACATGG GCGAACGTTA CAAGACCAAA 3780 CATTTGAAGG TCGAGGTGCA GAAAATCGTG CCGTACATCG GACTCCTCGT CTCCGCTGAG 3840 CAGGTCGTCC TCGACCCTGT CAGGAGCGCT CTCAAGATAT TTGGGCGCTG CTACACAAGC 3900 GAACTCCTTT ACTCCAAGTA CGTGGAGGCT GTGAGAGACA TCACCAAGGG CTGGAGTGAC 3960 j SUBSTITUTE SHEET 107 GCCCGCTACC ACAGCCTCCT GTGCCACATG TCAGCATGCT ACTACAATTA CGCGCCGGAG 4020 TCTGCGGCGT ACATCATCGA CGCTGTTGTT CGCTTTGGGC GCGGCGACTT CCCGTTTGAA 4080 CAACTGCGCG TGGTGCGTGC CCATGTGCAG GCACCCGACG CTTACAGCAG CACGTATCCG 4140 GCTAACGTGC GCGCATCGTG CCTTGACCAC GTCTTCGAGC CCCGCCAGGC CGCCGCCCCG 4200 GCAGGTTTCG TTGCGACATG TGCGAAGCCG GAAACGCCTT CTTCACTTAC CGCGA A AGCT 4260 GGTGTTTCTG CGACTACAAG CCACGTTGCG ACTGGGACTG CGCCCCCGGA GTCTCCATGG 4320 GATGCACCTG CAGCCAACAG CTTTTCGGAG TTATTGACAC CGGAGACCCC GTCCACATCA 4380 TCCTCGCCGT CATCGTCTTC ATCGGACTCC TCTACATCGT GTGGAAGGTC GCTCAGTGGT 4440 GGAGACACCG C A AGGACCAC AGAAGACTTG AACAGCAGAA AGCCGCCTTC GCAAGACAGG 4500 CAATCACGCT CGTCTGA ATG TCT GGA CAG AAG CGG AGA AAG GAC AGG CAG Met Ser Gly Gin Lys Arg Arg Lys Asp Arg Gin 4550 1 5 10 ΪPIhHe SV T aTl ^Asn Cry GC s P°r C o HS A i C s C T y GC s T S C e C r G GA lu G C P C r C o L CT eu C AHTTe CLTeuC AHTTe TPhTCe GGGlA y 4598 15 20 25 L L yJΪs t S eerr lSeerr τT5hr GG?lSy A?sp AΛ G rg G A5?la C A^sp JCy GΥ s A CG r C g A CG rg C T Cy GC s A GA sp C A H TG e AThCCr 4646 JU 35 40 PPhhee HCAiCs 4694 GCC GGA CTT TGC GCC TTT CCT GGG TTC CCA GTC TGC CCG TGC TGT CTC Ala Gly Leu Cys Ala Phe Pro Gly Phe Pro Val Cys Pro Cys Cys Leu 4742 65 70 75 GGlliu AA?l * a SVatl SP C r G o CPCrCo 4790 A A U l a P P rroo £ A rSg vVaSl G %l£u Grly G A?rg T A ^sp A ∞ rg A AG r A g G P C r G o A Th CC r G GG l A y A S G e T r G CA inG GGGlA y 4838 95 100 105 G Gllyv SPr™o Gr?l A y A? A sp C So G er C Gl,y° G.l, 1 y A CG r T g P CG r A o G GG ly C T S C e T r A GspT GCAinG ACGrGg CPCrGo TLTeAu 4886 ■LiU 115 120 Seerr SSeerr GG^lu AΪJsSp rGtlu A ^la 1P1h°e ?L T eu C A ,°r A g A CG rg C A H TC e G CA in A A A r G g G LCTeAu LCTeuC ALA y Gs 4934 1 130 135 J AAASπC TAAGGGATGC TCACAATCCA CCTCTTTTCC TGCCCCGAGT GCGGATTACC 4987 140 AGGCCCGCGA CTGCCAGACA GTCCGAGTCT GCCGCGCCGC TGCAGAGATG GCGCGCTCAT 5047 GTATTCACGA GCCGTTGGCT TCATCTGCCG CCAGTGCCGA CTTGAAGCGC ATACGCTCTA 5107 SUBSTITUTE SHEET CCTCGGACTC TGTTCCCGAT GTAAAGATCA GCAAGAGCGC ATGAAGGAAC AAAATTAGTT 5167 TCCTTGTTCG TAAACAAGGT GGTCCCTCCC ATTGAGGTAA AGACTCTGGT GAGTCCTCAA 5227 CGTTACTCGT TGAGTCTGCT GCGGTTCGAT TCCATTCCCA AGCAGCAAAG GGTGCGCAAC 5287 TAGTACGGCG CCCCCTGGGA TACCA 5312 (2) INFORMATION FOR SEQ ID NO:44: (i) SEOUENCE CHARACTERISTICS: (A) LENGTH: 140 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44: Met Ser Gly Gin Lys Arg Arg Lys Asp Arg Gin Phe Val Asn Cys Pro 1 5 10 . 15 His Cys Ser Glu Pro Leu He Leu He Phe Gly Lys Ser Ser Thr Gl y 20 25 30 Asp Arg Ala Asp Cys Arg Arg Cys Asp He Thr Phe Gly Asn Pro He J- 40 45 Leu Arg His Gly Pro Gly Cys Arg Glu Asp His Ala Gly Leu Cys Ala 50 55 60 Phe Pro Gly Phe Pro Val Cys Pro Cys Cys Leu Glu Ala Val Pro Ala 65 70 75 80 Pro His Asp Cys Pro Leu Glu Arg Ser His Pro Ala Pro Arg Val Glu 85 90 95 Gly Arg Asp Arg Arg Pro Thr Gly Ser Gin Gly Gly Pro Gly Asp Ser 1UU 105 HO Gly Gly Arg Pro Gly Ser Asp Gin Arg Pro Leu Ser Ser Glu Asp Glu 115 120 125 Ala ? ™ Leu Ar S Arg He Gin Arg Leu Leu Lys Asn 13 0 135 1 0 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:45: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 5312 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 4944..5162 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45: GTTCTGCCTC CCCCGGACGG TAAATATAGG GGAACAATGT ACGCGAAAGC GACAGACGTG 60 GCGCGTGTCT ACGCCGCGGC AGATGTCGCC TACGCGAACG TACTGCAGCA GAGAGCAGTC 120 AAGTTGGACT TCGCCCCGCC ACTGAAGGCA CTAGAAACCC TCCACAGACT GTACTATCCG 180 CTGCGCTTCA AAGGGGGCAC TTTACCCCCG ACACAACACC CGATCCTGGC CGGGCACCAA 240 CGTGTCGCAG AAGAGGTTCT GCACAATTTC GCCAGGGGAC GTAGCACAGT GCTCGAGATA 300 GGGCCGTCTC TGCACAGCGC ACTTAAGCTA CATGGGGCAC CGAACGCCCC CGTCGCAGAC 360 TATCACGGGT GCACCAAGTA CGGCACCCGC GACGGCTCGC GACACATTAC GGCCTTAGAG 420 TCTAGATCCG TCGCCACAGG CCGGCCCGAG TTCAAGGCCG ACGCCTCACT GCTCGCCAAC 480 GGCATTGCCT CCCGCACCTT CTGCGTCGAC GGAGTCGGCT CTTGCGCGTT CAAATCGCGC 540 GTTGGAATTG CCAATCACTC CCTCTATGAC GTGACCCTAG AGGAGCTGGC CAATGCGTTT 600 GAGAACCACG GACTTCACAT GGTCCGCGCG TTCATGCACA TGCCAGAAGA GCTGCTCTAC 660 ATGGACAACG TGGTTAATGC CGAGCTCGGC TACCGCTTCC ACGTTATTGA AGAGCCTATG 720 GCTGTGAAGG ACTGCGCATT CCAGGGGGGG GACCTCCGTC TCCACTTCCC TGAGTTGGAC 780 TTCATCAACG AGAGCCAAGA GCGGCGCATC GAGAGGCTGG CCGCCCGCGG CTCCTACTCC 840 AGACGCGCCG TCATTTTCTC CGGCGACGAC GACTGGGGTG ATGCGTACTT ACACGACTTC 900 CACACATGGC TCGCCTACCT ACTGGTGAGG AACTACCCCA CTCCGTTTGG TTTCTCACTC 960 CATATAGAAG TCCAGAGGCG CCACGGCTCC AGCATTGAGC TGCGCATCAC TCGCGCGCCA 1020 CCTGGAGACC GCATGCTGGC CGTCGTCCCA AGGACGTCCC AAGGCCTCTG CAGAATCCCA 1080 AACATCTTTT ATTACGCCGA CGCGTCGGGC ACTGAGCATA AGACCATCCT TACGTCACAG 1140 CACAAAGTCA ACATGCTGCT CAATTTTATG CAAACGCGTC CTGAGAAGGA ACTAGTCGAC 1200 ATGACCGTCT TGATGTCGTT CGCGCGCGCT AGGCTGCGCG CGATCGTGGT CGCCTCAGAA 1260 j SUBSTITUTE SHEET GTCACCGAGA GCTCCTGGAA CATCTCACCG GCTGACCTGG TCCGCACTGT CGTGTCTCTT 1320 TACGTCCTCC ACATCATCGA GCGCCGAAGG GCTGCGGTCG CTGTCAAGAC CGCCAAGGAC 1380 GACGTCTTTG GAGAGACTTC GTTCTGGGAG AGTCTCAAGC ACGTCTTGGG CTCCTGTTGC 1440 GGTCTGCGCA ACCTCAAAGG CACCGACGTC GTCTTTACTA AGCGCGTCGT CGATAAGTAC 1500 CGAGTCCACT CGCTCGGAGA CATAATCTGC GACGTCCGCC TGTCCCCTGA ACAGGTCGGC 1560 TTCCTGCCGT CCCGCGTACC ACCTGCCCGC GTCTTTCACG ACAGGGAAGA GCTTGAGGTC 1620 CTTCGCGAAG CTGGCTGCTA CAACGAACGT CCGGTACCTT CCACTCCTCC TGTGGAGGAG 1680 CCCCAAGGTT TCGACGCCGA CTTGTGGCAC GCGACCGCGG CCTCACTCCC CGAGTACCGC 1740 GCCACCTTGC AGGCAGGTCT CAACACCGAC GTCAAGCAGC TCAAGATCAC CCTCGAGAAC 1800 GCCCTCAAGA CCATCGACGG GCTCACCCTC TCCCCAGTCA GAGGCCTCGA GATGTACGAG 1860 GGCCCGCCAG GCAGCGGCAA GACGGGCACC CTCATCGCCG CCCTTGAGGC CGCGGGCGGT 1920 AAAGCACTTT ACGTGGCACC CACCAGAGAA CTGAGAGAGG CTATGGACCG GCGGATCAAA 1980 CCGCCGTCCG CCTCGGCTAC GCAACATGTC GCCCTTGCGA TTCTCCGTCG TGCCACCGCC 2040 GAGGGCGCCC CTTTCGCTAC CGTGGTTATC GACGAGTGCT TCATGTTCCC GCTCGTGTAC 2100 GTCGCGATCG TGCACGCCTT GTCCCCGAGC TCACGAATAG TCCTTGTAGG GGACGTCCAC 2160 CAAATCGGGT TTATAGACTT CCAAGGCACA AGCGCGAACA TGCCGCTCGT TCGCGACGTC 2220 GTTAAGCAGT GCCGTCGGCG CACTTTCAAC CAAACCAAGC GCTGTCCGGC CGACGTCGTT 2280 GCCACCACGT TTTTCCAGAG CTTGTACCCC GGGTGCACAA CCACCTCAGG GTGCGTCGCA 2340 TCCATCAGCC ACGTCGCCCC AGACTACCGC AACAGCCAGG CGCAAACGCT CTGCTTCACG 2400 CAGGAGGAAA AGTCGCGCCA CGGGGCTGAG GGCGCGATGA CTGTGCACGA AGCGCAGGGA 2460 CGCACTTTTG CGTCTGTCAT TCTGCATTAC AACGGCTCCA CAGCAGAGCA GAAGCTCCTC 2520 GCTGAGAAGT CGCACCTTCT AGTCGGCATC ACGCGCCACA CCAACCACCT GTACATCCGC 2580 GACCCGACAG GTGACATTGA GAGACAACTC AACCATAGCG CGAAAGCCGA GGTGTTTACA 2640 GACATCCCTG CACCCCTGGA GATCACGACT GTCAAACCGA GTGAAGAGGT GCAGCGCAAC 2700 GAAGTGATGG CAACGATACC CCCGCAGAGT GCCACGCCGC ACGGAGCAAT CCATCTGCTC 2760 CGCAAGAACT TCGGGGACCA ACCCGACTGT GGCTGTGTCG CTTTGGCGAA GACCGGCTAC 2820 GAGGTGTTTG GCGGTCGTGC CAAAATCAAC GTAGAGCTTG CCGAACCCGA CGCGACCCCG 2880 AAGCCGCATA GGGCGTTCCA GGAAGGGGTA CAGTGGGTCA AGGTCACCAA CGCGTCTAAC 2940 AAACACCAGG CGCTCCAGAC GCTGTTGTCC CGCTACACCA AGCGAAGCGC TGACCTGCCG 3000 CTACACGAAG CTAAGGAGGA CGTCAAACGC ATGCTAAACT CGCTTGACCG ACATTGGGAC 3060 SUBSTITUTE SHEET SUBSTITUTE SHEET AAAGGCTACT CAAGAACTAA GGG ATG CTC ACA ATC CAC CTC TTT TCC TGC 4970 Met Leu Thr He His Leu Phe Ser Cys 1 5 CCC GAG TGC GGA TTA CCA GGC CCG CGA CTG CCA GAC AGT CCG AGT CTG 5018 Pro Glu Cys Gly Leu Pro Gly Pro Arg Leu Pro Asp Ser Pro Ser Leu 10 15 20 25 CCG CGC CGC TGC AGA GAT GGC GCG CTC ATG TAT TCA CGA GCC GTT GGC 5066 Pro Arg Arg Cys Arg Asp Gly Ala Leu Met Tyr Ser Arg Ala Val Gly 30 35 40 TTC ATC TGC CGC CAG TGC CGA CTT GAA GCG CAT ACG CTC TAC CTC GGA 5114 Phe He Cys Arg Gin Cys Arg Leu Glu Ala His Thr Leu Tyr Leu Gly 45 50 55 CTC TGT TCC CGA TGT AAA GAT CAG CAA GAG CGC ATG AAG GAA CAA AAT 5162 Leu Cys Ser Arg Cys Lys Asp Gin Gin Glu Arg Met Lys Glu Gin Asn 60 65 70 TAGTTTCCTT GTTCGTAAAC AAGGTGGTCC CTCCCATTGA GGTAAAGACT CTGGTGAGTC 5222 CTCAACGTTA CTCGTTGAGT CTGCTGCGGT TCGATTCCAT TCCCAAGCAG CAAAGGGTGC 5282 GCAACTAGTA CGGCGCCCCC TGGGATACCA 5312 (2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 73 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46: Met Leu Thr He His Leu Phe Ser Cys Pro Glu Cys Gly Leu Pro Gly 1 5 10 15 Pro Arg Leu Pro Asp Ser Pro Ser Leu Pro Arg Arg Cys Arg Asp Gly 20 25 30 Ala Leu Met Tyr Ser Arg Ala Val Gly Phe He Cys Arg Gin Cys Arg 35 40 45 Leu Glu Ala His Thr Leu Tyr Leu Gly Leu Cys Ser Arg Cys Lys Asp 50 55 60 Gin Gin Glu Arg Met Lys Glu Gin Asn 65 70 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:47: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2478 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 283..753 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47: GTTTTTCTTT CTTTACCAAG TGTGGTAAAA TTTAAACAAA GAAGAAAACC AGGACCGTAA 60 CCCGGCCCTT ACACACCTCG AGTCCGTGAC CACCGGATTA TACGTCGCCC ACCACACGGC 120 GCCTTTTCCG ACCACTCTCG AGAGTCGTTG GGAGTTTCGT CCGTGACCAC CCGGTTGGCA 180 GTCGACAGAC GCTTCCGGAC CACTAGAACC TCCTCGAGCG ACGCACACAC AGCACACACA 240 CCGCCTTAGC TGCACCTACG GCAGCGTTGA TAGCGCGGAT TT ATG AGC GAG CAC 294 Met Ser Glu His 1 ACC ATC GCC CAC TCC ATC ACA TTA CCA CCC GGT TAC ACC CTT GCC CTA 342 Thr He Ala His Ser He Thr Leu Pro Pro Gly Tyr Thr Leu Ala Leu 5 10 15 20 ATA CCC CCT GAA CCT GAA GCA GGA TGG GAG ATG CTG GAG TGG CGT CAC 390 He Pro Pro Glu Pro Glu Ala Gly Trp Glu Met Leu Glu Trp Arg His 25 30 35 AGC GAC CTC ACA ACC GTC GCG GAA CCC GTA ACG TTC GGG TCA GCG CCA 438 Ser Asp Leu Thr Thr Val Ala Glu Pro Val Thr Phe Gly Ser Ala Pro 40 45 50 ACA CCG TCA CCG TCA ATG GTA GAA GAA ACC AAC GGC GTC GGA CCG GAA 486 Thr Pro Ser Pro Ser Met Val Glu Glu Thr Asn Gly Val Gly Pro Glu 55 60 65 GGC AAG TTT CTC CCC CTG ACA ATT TCA CCG CTG CTG CAC AAG ACC TCG 534 Gly Lys Phe Leu Pro Leu Thr He Ser Pro Leu Leu His Lys Thr Ser 70 75 80 CGC AAA GCC TTG ACG CCA ACA CCG TCA CTT TCC CCG CTA ACA TCT CTA 582 Arg Lys Ala Leu Thr Pro Thr Pro Ser Leu Ser Pro Leu Thr Ser Leu 85 90 95 100 GCA TGC CCG AAT TCC GGA ATT GGG CCA AGG GAA AGA TCG ACC TCG ACT 630 Ala Cys Pro Asn Ser Gly He Gly Pro Arg Glu Arg Ser Thr Ser Thr 105 110 115 SUBSTITUTE SH IEEEETT CCG ATT CCA TCG GCT GGT ACT TCA AGT ACC TTG ACC CAG CGG GTG CTA 678 Pro He Pro Ser Ala Gly Thr Ser Ser Thr Leu Thr Gin Arg Val Leu 120 125 130 CAG AGT CTG CGC GCG CCG TCG GCG AGT ACT CGA AGA TCC CTG ACG GCC 726 Gin Ser Leu Arg Ala Pro Ser Ala Ser Thr Arg Arg Ser Leu Thr Ala 135 140 145 TCG TCA AGT TCT CCG TCG ACG CAG AGA TAAGAGAGAT CTATAACGAG 773 Ser Ser Ser Ser Pro Ser Thr Gin Arg 150 155 GAGTGCCCCG TCGTCACTGA CGTGTCCGTC CCCCTCGACG GCCGCCAGTG GAGCCTCTCG 833 ATTTTCTCCT TTCCGATGTT CAGAACCGCC TACGTCGCCG TAGCGAACGT CGAGAACAAG 893 GAGATGTCGC TCGACGTTGT CAACGACCTC ATCGAGTGGC TCAACAATCT CGCCGACTGG 953 CGTTATGTCG TTGACTCTGA ACAGTGGATT AACTTCACCA ATGACACCAC GTACTACGTC 1013 CGCATCCGCG TTCTACGTCC AACCTACGAC GTTCCAGACC CCACAGAGGG CCTTGTTCGC 1073 ACAGTCTCAG ACTACCGCCT CACTTATAAG GCGATAACAT GTGAAGCCAA CATGCCAACA 1133 CTCGTCGACC AAGGCTTTTG GATCGGCGGC CAGTACGCTC TCACCCCGAC TAGCCTACCG 1193 CAGTACGACG TCAGCGAGGC CTACGCTCTG CACACTTTGA CCTTCGCCAG ACCATCCAGC 1253 GCCGCTGCAC TCGCGTTTGT GTGGGCAGGT TTGCCACAGG GTGGCACTGC GCCTGCAGGC 1313 ACTCCAGCCT GGGAGCAGGC ATCCTCGGGT GGCTACCTCA CCTGGCGCCA CAACGGTACT 1373 ACTTTCCCAG CTGGCTCCGT TAGCTACGTT CTCCCTGAGG GTTTCGCCCT TGAGCGCTAC 1433 GACCCGAACG ACGGCTCTTG GACCGACTTC GCTTCCGCAG GAGACACCGT CACTTTCCGG 1493 CAGGTCGCCG TCGACGAGGT CGTTGTGACC AACAACCCCG CCGGCGGCGG CAGCGCCCCC 1553 ACCTTCACCG TGAGAGTGCC CCCTTCAAAC GCTTACACCA ACACCGTGTT TAGGAACACG 1613 CTCTTAGAGA CTCGACCCTC CTCTCGTAGG CTCGAACTCC CTATGCCACC TGCTGACTTT 1673 GGACAGACGG TCGCCAACAA CCCGAAGATC GAGCAGTCGC TTCTTAAAGA AACACTTGGC 1733 TGCTATTTGG TCCACTCCAA AATGCGAAAC CCCGTTTTCC AGCTCACGCC AGCCAGCTCC 1793 TTTGGCGCCG TTTCCTTCAA CAATCCGGGT TATGAGCGCA CACGCGACCT CCCGGACTAC 1853 ACTGGCATCC GTGACTCATT CGACCAGAAC ATGTCCACCG CTGTGGCCCA CTTCCGCTCA 1913 CTCTCCCACT CCTGCAGTAT CGTCACTAAG ACCTACCAGG GTTGGGAAGG CGTCACGAAC 1973 GTCAACACGC CTTTCGGCCA ATTCGCGCAC GCGGGCCTCC TCAAGAATGA GGAGATCCTC 2033 TGCCTCGCCG ACGACCTGGC CACCCGTCTC ACAGGTGTCT ACCCCGCCAC TGACAACTTC 2093 GCGGCCGCCG TTTCTGCCTT CGCCGCGAAC ATGCTGTCCT CCGTGCTGAA GTCGGAGGCA 2153 ACGTCCTCCA TCATCAAGTC CGTTGGCGAG ACTGCCGTCG GCGCGGCTCA GTCCGGCCTC 2213 SUBSTITUTE SHEET GCGAAGCTAC CCGGACTGCT AATGAGTGTA CCAGGGAAGA TTGCCGCGCG TGTCCGCGCG 2273 CGCCGAGCGC GCCGCCGCGC CGCTCGTGCC AATTAGTTTG CTCGCTCCTG TTTCGCCGTT 2333 TCGTAAAACG GCGTGGTCCC GCACATTACG CGTACCCTAA AGACTCTGGT GAGTCCCCGT 2393 CGTTACACGA CGGGTCTGCC GCGGTTCGAT TCCATTCCCA AGCGGCAAGA AGGACGTAGT 2453 TAGCTCTGCG TCCCTCGGGA TACCA 2478 (2) INFORMATION FOR SEQ ID NO:48: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 157 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48: Met Ser Glu His Thr He Ala His Ser He Thr Leu Pro Pro Gly Tyr 1 5 10 V Thr Leu Ala Leu He Pro Pro Glu Pro Glu Ala Gly Trp Glu Met Leu 20 25 30 Glu Trp Arg His Ser Asp Leu Thr Thr Val Ala Glu Pro Val Thr Phe 35 40 45 Gly Ser Ala Pro Thr Pro Ser Pro Ser Met Val Glu Glu Thr Asn Gly 50 55 60 Val Gly Pro Glu Gly Lys Phe Leu Pro Leu Thr He Ser Pro Leu Leu 65 70 75 80 His Lys Thr Ser Arg Lys Ala Leu Thr Pro Thr Pro Ser Leu Ser Pro 85 90 95 Leu Thr Ser Leu Ala Cys Pro Asn Ser Gly He Gly Pro Arg Glu Are 100 105 HO Ser Thr Ser Thr Pro He Pro Ser Ala Gly Thr Ser Ser Thr Leu Thr 115 120 125 Gin Arg Val Leu Gin Ser Leu Arg Ala Pro Ser Ala Ser Thr Are Are 130 135 140 Ser Leu Thr Ala Ser Ser Ser Ser Pro Ser Thr Gin Are 145 150 155 SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:49: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2478 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 366..2306 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49: GTTTTTCTTT CTTTACCAAG TGTGGTAAAA TTTAAACAAA GAAGAAAACC AGGACCGTAA 60 CCCGGCCCTT ACACACCTCG AGTCCGTGAC CACCGGATTA TACGTCGCCC ACCACACGGC 120 GCCTTTTCCG ACCACTCTCG AGAGTCGTTG GGAGTTTCGT CCGTGACCAC CCGGTTGGCA 180 GTCGACAGAC GCTTCCGGAC CACTAGAACC TCCTCGAGCG ACGCACACAC AGCACACACA 240 CCGCCTTAGC TGCACCTACG GCAGCGTTGA TAGCGCGGAT TTATGAGCGA GCACACCATC 300 GCCCACTCCA TCACATTACC ACCCGGTTAC ACCCTTGCCC TAATACCCCC TGAACCTGAA 360 GCAGG ATG GGA GAT GCT GGA GTG GCG TCA CAG CGA CCT CAC AAC CGT 407 Met Gly Asp Ala Gly Val Ala Ser Gin Arg Pro His Asn Are 1 5 10 A GC ? A C ? GT C GT CGG GTC AGC GCC AAC ACC GTC ACC GTC AAT 455 Arg Gly Thr Arg Asn Val Arg Val Ser Ala Asn Thr Val Thr Val Asn 1 20 25 30 GGT AGA AGA AAC CAA CGG CGT CGG ACC GGA AGG CAA GTT TCT CCC CCT 503 Gly Arg Arg Asn Gin Arg Arg Arg Thr Gly Arg Gin Val Ser Pro Pro 35 40 45 GAC AAT TTC ACC GCT GCT GCA CAA GAC CTC GCG CAA AGC CTT GAC GCC 551 Asp Asn Phe Thr Ala Ala Ala Gin Asp Leu Ala Gin Ser Leu Asp Ala 50 55 60 AAC ACC GTC ACT TTC CCC GCT AAC ATC TCT AGC ATG CCC GAA TTC CGG 599 Asn Thr Val Thr Phe Pro Ala Asn He Ser Ser Met Pro Glu Phe Are 65 70 75 6 AAT TGG GCC AAG GGA AAG ATC GAC CTC GAC TCC GAT TCC ATC GGC TGG 647 Asn Trp Ala Lys Gly Lys He Asp Leu Asp Ser Asp Ser He Gl y Tr p oϋ 85 90 TAC TTC AAG TAC CTT GAC CCA GCG GGT GCT ACA GAG TCT GCG CGC GCC 695 Tyr Phe Lys Tyr Leu Asp Pro Ala Gly Ala Thr Glu Ser Ala Arg Ala 95 100 105 HO GTC GGC GAG TAC TCG AAG ATC CCT GAC GGC CTC GTC AAG TTC TCC GTC 743 Val Gly Glu Tyr Ser Lys He Pro Asp Gly Leu Val Lys Phe Ser Val 115 120 125 SUBSTITUTE SHEET 791 839 887 935 983 1031 1079 1127 1175 1223 1271 1319 GCC TGG GAG CAG GCA TCC TCG GGT GGC TAC CTC ACC TGG CGC CAC AAC 1367 Ala Trp Glu Gin Ala Ser Ser Gly Gly Tyr Leu Thr Trp Arg His Asn 320 325 330 GGT ACT ACT TTC CCA GCT GGC TCC GTT AGC TAC GTT CTC CCT GAG GGT 1415 Gly Thr Thr Phe Pro Ala Gly Ser Val Ser Tyr Val Leu Pro Glu Gly 335 340 345 350 TTC GCC CTT GAG CGC TAC GAC CCG AAC GAC GGC TCT TGG ACC GAC TTC 1463 Phe Ala Leu Glu Arg Tyr Asp Pro Asn Asp Gly Ser Trp Thr Asp Phe 355 360 365 i SUBSTITUTE SHEET GCT TCC GCA GGA GAC ACC GTC ACT TTC CGG CAG GTC GCC GTC GAC GAG 1511 Ala Ser Ala Gly Asp Thr Val Thr Phe Arg Gin Val Ala Val Asp Glu 370 375 380 GTC GTT GTG ACC AAC AAC CCC GCC GGC GGC GGC AGC GCC CCC ACC TTC 1559 Val Val Val Thr Asn Asn Pro Ala Gly Gly Gly Ser Ala Pro Thr Phe 385 390 395 ACC GTG AGA GTG CCC CCT TCA AAC GCT TAC ACC AAC ACC GTG TTT AGG 1607 Thr Val Arg Val Pro Pro Ser Asn Ala Tyr Thr Asn Thr Val Phe Arg 400 405 410 AAC ACG CTC TTA GAG ACT CGA CCC TCC TCT CGT AGG CTC GAA CTC CCT 1655 Asn Thr Leu Leu Glu Thr Arg Pro Ser Ser Arg Arg Leu Glu Leu Pro 415 420 425 430 ATG CCA CCT GCT GAC TTT GGA CAG ACG GTC GCC AAC AAC CCG AAG ATC 1703 Met Pro Pro Ala Asp Phe Gly Gin Thr Val Ala Asn Asn Pro Lys He 435 440 445 GAG CAG TCG CTT CTT AAA GAA ACA CTT GGC TGC TAT TTG GTC CAC TCC 1751 Glu Gin Ser Leu Leu Lys Glu Thr Leu Gly Cys Tyr Leu Val His Ser 450 455 460 AAA ATG CGA AAC CCC GTT TTC CAG CTC ACG CCA GCC AGC TCC TTT GGC 1799 Lys Met Arg Asn Pro Val Phe Gin Leu Thr Pro Ala Ser Ser Phe Gly 465 470 475 GCC GTT TCC TTC AAC AAT CCG GGT TAT GAG CGC ACA CGC GAC CTC CCG 1847 Ala Val Ser Phe Asn Asn Pro Gly Tyr Glu Arg Thr Arg Asp Leu Pro 480 485 490 GAC TAC ACT GGC ATC CGT GAC TCA TTC GAC CAG AAC ATG TCC ACC GCT 1895 Asp Tyr Thr Gly He Arg Asp Ser Phe Asp Gin Asn Met Ser Thr Ala 495 500 505 510 GTG GCC CAC TTC CGC TCA CTC TCC CAC TCC TGC AGT ATC GTC ACT AAG 1943 Val Ala His Phe Arg Ser Leu Ser His Ser Cys Ser He Val Thr Lys 515 520 525 ACC TAC CAG GGT TGG GAA GGC GTC ACG AAC GTC AAC ACG CCT TTC GGC 1991 Thr Tyr Gin Gly Trp Glu Gly Val Thr Asn Val Asn Thr Pro Phe Gly 530 535 540 CAA TTC GCG CAC GCG GGC CTC CTC AAG AAT GAG GAG ATC CTC TGC CTC 2039 Gin Phe Ala His Ala Gly Leu Leu Lys Asn Glu Glu He Leu Cys Leu 545 550 555 GCC GAC GAC CTG GCC ACC CGT CTC ACA GGT GTC TAC CCC GCC ACT GAC 2087 Ala Asp Asp Leu Ala Thr Arg Leu Thr Gly Val Tyr Pro Ala Thr Asp 560 565 570 AAC TTC GCG GCC GCC GTT TCT GCC TTC GCC GCG AAC ATG CTG TCC TCC 2135 Asn Phe Ala Ala Ala Val Ser Ala Phe Ala Ala Asn Met Leu Ser Ser 575 580 585 590 GTG CTG AAG TCG GAG GCA ACG TCC TCC ATC ATC AAG TCC GTT GGC GAG 2183 Val Leu Lys Ser Glu Ala Thr Ser Ser He He Lys Ser Val Gly Glu 595 600 605 SUBSTITUTE SHEET ACT GCC GTC GGC GCG GCT CAG TCC GGC CTC GCG AAG CTA CCC GGA CTG 2231 Thr Ala Val Gly Ala Ala Gin Ser Gly Leu Ala Lys Leu Pro Gly Leu 610 615 620 CTA ATG AGT GTA CCA GGG AAG ATT GCC GCG CGT GTC CGC GCG CGC CGA 2279 Leu Met Ser Val Pro Gly Lys He Ala Ala Arg Val Arg Ala Arg Arg 625 630 635 GCG CGC CGC CGC GCC GCT CGT GCC AAT TAGTTTGCTC GCTCCTGTTT 2326 Ala Arg Arg Arg Ala Ala Arg Ala Asn 640 645 CGCCGTTTCG TAAAACGGCG TGGTCCCGCA CATTACGCGT ACCCTAAAGA CTCTGGTGAG 2386 TCCCCGTCGT TACACGACGG GTCTGCCGCG GTTCGATTCC ATTCCCAAGC GGCAAGAAGG 2446 ACGTAGTTAG CTCTGCGTCC CTCGGGATAC CA 2478 (2) INFORMATION FOR SEQ ID NO:50: (i) SEOUENCE CHARACTERISTICS: (A) LENGTH: 647 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50: Met Gly Asp Ala Gly Val Ala Ser Gin Arg Pro His Asn Arg Are Gl y 1 5 10 15 Thr Arg Asn Val Arg Val Ser Ala Asn Thr Val Thr Val Asn Gl y Are 20 25 30 Arg Asn Gin Arg Arg Arg Thr Gly Arg Gin Val Ser Pro Pro Asp Asn 35 40 45 Phe Thr Ala Ala Ala Gin Asp Leu Ala Gin Ser Leu Asp Ala Asn Thr 50 55 60 Val Thr Phe Pro Ala Asn He Ser Ser Met Pro Glu Phe Arg Asn Tr p 65 70 75 8δ Ala Lys Gly Lys He Asp Leu Asp Ser Asp Ser He Gly Trp Tyr Phe 85 90 95 Lys Tyr Leu Asp Pro Ala Gly Ala Thr Glu Ser Ala Arg Ala Val Gl y 100 105 HO Glu Tyr Ser Lys He Pro Asp Gly Leu Val Lys Phe Ser Val Asp Ala 115 120 125 Glu lie Arg Glu He Tyr Asn Glu Glu Cys Pro Val Val Thr Asp Val 130 135 140 Ser Val Pro Leu Asp Gly Arg Gin Trp Ser Leu Ser He Phe Ser Phe 145 150 155 160 SUBSTITUTE SHEET Pro Met Phe Arg Thr Ala Tyr Val Ala Val Ala Asn Val Glu Asn Lys 165 170 175 Glu Met Ser Leu Asp Val Val Asn Asp Leu He Glu Trp Leu Asn Asn 180 185 190 Leu Ala Asp Trp Arg Tyr Val Val Asp Ser Glu Gin Trp He Asn Phe 195 200 205 Thr Asn Asp Thr Thr Tyr Tyr Val Arg He Arg Val Leu Arg Pro Thr 210 215 220 Tyr Asp Val Pro Asp Pro Thr Glu Gly Leu Val Arg Thr Val Ser Asp 225 230 235 24b Tyr Arg Leu Thr Tyr Lys Ala He Thr Cys Glu Ala Asn Met Pro Thr 245 250 255 Leu Val Asp Gin Gly Phe Trp He Gly Gly Gin Tyr Ala Leu Thr Pro 260 265 270 Thr Ser Leu Pro Gin Tyr Asp Val Ser Glu Ala Tyr Ala Leu His Thr 275 280 285 Leu Thr Phe Ala Arg Pro Ser Ser Ala Ala Ala Leu Ala Phe Val Trp 290 295 300 Ala Gly Leu Pro Gin Gly Gly Thr Ala Pro Ala Gly Thr Pro Ala Trp 305 310 315 320 Glu Gin Ala Ser Ser Gly Gly Tyr Leu Thr Trp Arg His Asn Gly Thr 325 330 335 Thr Phe Pro Ala Gly Ser Val Ser Tyr Val Leu Pro Glu Gly Phe Ala 340 345 350 Leu Glu Arg Tyr Asp Pro Asn Asp Gly Ser Trp Thr Asp Phe Ala Ser 355 360 365 Ala Gly Asp Thr Val Thr Phe Arg Gin Val Ala Val Asp Glu Val Val 370 375 380 Val Thr Asn Asn Pro Ala Gly Gly Gly Ser Ala Pro Thr Phe Thr Val 385 390 395 400 Arg Val Pro Pro Ser Asn Ala Tyr Thr Asn Thr Val Phe Arg Asn Thr 405 410 415 Leu Leu Glu Thr Arg Pro Ser Ser Arg Arg Leu Glu Leu Pro Met Pro 420 425 430 Pro Ala Asp Phe Gly Gin Thr Val Ala Asn Asn Pro Lys He Glu Gin 435 440 445 Ser Leu Leu Lys Glu Thr Leu Gly Cys Tyr Leu Val His Ser Lys Met 450 455 460 Arg Asn Pro Val Phe Gin Leu Thr Pro Ala Ser Ser Phe Gly Ala Val 465 470 475 480 SUBSTITUTE SHEET Ser Phe Asn Asn Pro Gly Tyr Glu Arg Thr Arg Asp Leu Pro Asp Tyr 485 490 495 Thr Gly He Arg Asp Ser Phe Asp Gin Asn Met Ser Thr Ala Val Ala 500 505 510 His Phe Arg Ser Leu Ser His Ser Cys Ser He Val Thr Lys Thr Tyr 515 520 525 Gin Gly Trp Glu Gly Val Thr Asn Val Asn Thr Pro Phe Gly Gin Phe 530 535 540 Ala His Ala Gly Leu Leu Lys Asn Glu Glu He Leu Cys Leu Ala As 545 550 555 56 Asp Leu Ala Thr Arg Leu Thr Gly Val Tyr Pro Ala Thr Asp Asn Phe 565 570 575 Ala Ala Ala Val Ser Ala Phe Ala Ala Asn Met Leu Ser Ser Val Leu 580 585 590 Lys Ser Glu Ala Thr Ser Ser He He Lys Ser Val Gly Glu Thr Ala 595 600 605 Val Gly Ala Ala Gin Ser Gly Leu Ala Lys Leu Pro Gly Leu Leu Met 610 615 620 Ser Val Pro Gly Lys He Ala Ala Arg Val Arg Ala Arg Arg Ala Arg 625 630 635 640 Arg Arg Ala Ala Arg Ala Asn 645 (2) INFORMATION FOR SEQ ID NO:51: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2479 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 283..2307 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51: GTTTTTCTTT CTTTACCAAG TGTGGTAAAA TTTAAACAAA GAAGAAAACC AGGACCGTAA 60 CCCGGCCCTT ACACACCTCG AGTCCGTGAC CACCGGATTA TACGTCGCCC ACCACACGGC 120 GCCTTTTCCG ACCACTCTCG AGAGTCGTTG GGAGTTTCGT CCGTGACCAC CCGGTTGGCA 180 GTCGACAGAC GCTTCCGGAC CACTAGAACC TCCTCGAGCG ACGCACACAC AGCACACACA 240 SUBSTITUTE SHEET 294 342 390 438 486 534 582 630 678 726 774 822 870 918 966 SUBSTITUTE SHEET GAC TCT GAA CAG TGG ATT AAC TTC ACC AAT GAC ACC ACG TAC TAC GTC 1014 Asp Ser Glu Gin Trp He Asn Phe Thr Asn Asp Thr Thr Tyr Tyr Val 230 235 240 CGC ATC CGC GTT CTA CGT CCA ACC TAC GAC GTT CCA GAC CCC ACA GAG 1062 Arg He Arg Val Leu Arg Pro Thr Tyr Asp Val Pro Asp Pro Thr Glu 245 250 * 255 260 GGC CTT GTT CGC ACA GTC TCA GAC TAC CGC CTC ACT TAT AAG GCG ATA 1110 Gly Leu Val Arg Thr Val Ser Asp Tyr Arg Leu Thr Tyr Lys Ala He 265 270 275 ACA TGT GAA GCC AAC ATG CCA ACA CTC GTC GAC CAA GGC TTT TGG ATC 1158 Thr Cys Glu Ala Asn Met Pro Thr Leu Val Asp Gin Gly Phe Trp He 280 285 290 GGC GGC CAG TAC GCT CTC ACC CCG ACT AGC CTA CCG CAG TAC GAC GTC 1206 Gly Gly Gin Tyr Ala Leu Thr Pro Thr Ser Leu Pro Gin Tyr Asp Val 295 300 305 AGC GAG GCC TAC GCT CTG CAC ACT TTG ACC TTC GCC AGA CCA TCC AGC 1254 Ser Glu Ala Tyr Ala Leu His Thr Leu Thr Phe Ala Arg Pro Ser Ser 310 315 320 GCC GCT GCA CTC GCG TTT GTG TGG GCA GGT TTG CCA CAG GGT GGC ACT 1302 Ala Ala Ala Leu Ala Phe Val Trp Ala Gly Leu Pro Gin Gly Gly Thr 325 330 335 340 GCG CCT GCA GGC ACT CCA GCC TGG GAG CAG GCA TCC TCG GGT GGC TAC 1350 Ala Pro Ala Gly Thr Pro Ala Trp Glu Gin Ala Ser Ser Gly Gly Tyr 345 350 355 CTC ACC TGG CGC CAC AAC GGT ACT ACT TTC CCA GCT GGC TCC GTT AGC 1398 Leu Thr Trp Arg His Asn Gly Thr Thr Phe Pro Ala Gly Ser Val Ser 360 365 370 TAC GTT CTC CCT GAG GGT TTC GCC CTT GAG CGC TAC GAC CCG AAC GAC 1446 Tyr Val Leu Pro Glu Gly Phe Ala Leu Glu Arg Tyr Asp Pro Asn Asp 375 380 385 GGC TCT TGG ACC GAC TTC GCT TCC GCA GGA GAC ACC GTC ACT TTC CGG 1494 Gly Ser Trp Thr Asp Phe Ala Ser Ala Gly Asp Thr Val Thr Phe Are 390 395 400 CAG GTC GCC GTC GAC GAG GTC GTT GTG ACC AAC AAC CCC GCC GGC GGC 1542 Gin Val Ala Val Asp Glu Val Val Val Thr Asn Asn Pro Ala Gly Gly 405 410 415 420 GGC AGC GCC CCC ACC TTC ACC GTG AGA GTG CCC CCT TCA AAC GCT TAC 1590 Gly Ser Ala Pro Thr Phe Thr Val Arg Val Pro Pro Ser Asn Ala Tyr 425 430 435 ACC AAC ACC GTG TTT AGG AAC ACG CTC TTA GAG ACT CGA CCC TCC TCT 1638 Thr Asn Thr Val Phe Arg Asn Thr Leu Leu Glu Thr Arg Pro Ser Ser 440 445 450 CGT AGG CTC GAA CTC CCT ATG CCA CCT GCT GAC TTT GGA CAG ACG GTC 1686 Arg Arg Leu Glu Leu Pro Met Pro Pro Ala Asp Phe Gly Gin Thr Val 455 460 465 SUBSTITUTE SHEET GCC AAC AAC CCG AAG ATC GAG CAG TCG CTT CTT AAA GAA ACA CTT GGC 1734 Ala Asn Asn Pro Lys He Glu Gin Ser Leu Leu Lys Glu Thr Leu Gly 470 475 480 TGC TAT TTG GTC CAC TCC AAA ATG CGA AAC CCC GTT TTC CAG CTC ACG 1782 Cys Tyr Leu Val His Ser Lys Met Arg Asn Pro Val Phe Gin Leu Thr 485 490 495 500 CCA GCC AGC TCC TTT GGC GCC GTT TCC TTC AAC AAT CCG GGT TAT GAG 1830 Pro Ala Ser Ser Phe Gly Ala Val Ser Phe Asn Asn Pro Gly Tyr Glu 505 510 515 CGC ACA CGC GAC CTC CCG GAC TAC ACT GGC ATC CGT GAC TCA TTC GAC 1878 Arg Thr Arg Asp Leu Pro Asp Tyr Thr Gly He Arg Asp Ser Phe Asp 520 525 530 CAG AAC ATG TCC ACC GCT GTG GCC CAC TTC CGC TCA CTC TCC CAC TCC 1926 Gin Asn Met Ser Thr Ala Val Ala His Phe Arg Ser Leu Ser His Ser 535 540 545 TGC AGT ATC GTC ACT AAG ACC TAC CAG GGT TGG GAA GGC GTC ACG AAC 1974 Cys Ser He Val Thr Lys Thr Tyr Gin Gly Trp Glu Gly Val Thr Asn 550 555 560 GTC AAC ACG CCT TTC GGC CAA TTC GCG CAC GCG GGC CTC CTC AAG AAT 2022 Val Asn Thr Pro Phe Gly Gin Phe Ala His Ala Gly Leu Leu Lys Asn 565 570 575 580 GAG GAG ATC CTC TGC CTC GCC GAC GAC CTG GCC ACC CGT CTC ACA GGT 2070 Glu Glu He Leu Cys Leu Ala Asp Asp Leu Ala Thr Arg Leu Thr Gly 585 590 595 GTC TAC CCC GCC ACT GAC AAC TTC GCG GCC GCC GTT TCT GCC TTC GCC 2118 Val Tyr Pro Ala Thr Asp Asn Phe Ala Ala Ala Val Ser Ala Phe Ala 600 605 610 GCG AAC ATG CTG TCC TCC GTG CTG AAG TCG GAG GCA ACG TCC TCC ATC 2166 Ala Asn Met Leu Ser Ser Val Leu Lys Ser Glu Ala Thr Ser Ser He 615 620 625 ATC AAG TCC GTT GGC GAG ACT GCC GTC GGC GCG GCT CAG TCC GGC CTC 2214 He Lys Ser Val Gly Glu Thr Ala Val Gly Ala Ala Gin Ser Gly Leu 630 635 640 GCG AAG CTA CCC GGA CTG CTA ATG AGT GTA CCA GGG AAG ATT GCC GCG 2262 Ala Lys Leu Pro Gly Leu Leu Met Ser Val Pro Gly Lys He Ala Ala 645 650 655 660 CGT GTC CGC GCG CGC CGA GCG CGC CGC CGC GCC GCT CGT GCC AAT 2307 Arg Val Arg Ala Arg Arg Ala Arg Arg Arg Ala Ala Arg Ala Asn 665 670 675 TAGTTTGCTC GCTCCTGTTT CGCCGTTTCG TAAAACGGCG TGGTCCCGCA CATTACGCGT 2367 ACCCTAAAGA CTCTGGTGAG TCCCCGTCGT TACACGACGG GTCTGCCGCG GTTCGATTCC 2427 ATTCCCAAGC GGCAAGAAGG ACGTAGTTAG CTCTGCGTCC CTCGGGATAC CA 2479 j SUBSTITUTE SHEET (2) INFORMATION FOR SEQ ID NO:52: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 675 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52: Met Ser Glu His Thr He Ala His Ser He Thr Leu Pro Pro Gly Tyr 1 5 10 15 Thr Leu Ala Leu He Pro Pro Glu Pro Glu Ala Gly Trp Glu Met Leu 20 25 30 Glu Trp Arg His Ser Asp Leu Thr Thr Val Ala Glu Pro Val Thr Phe 35 40 45 Gly Ser Ala Pro Thr Pro Ser Pro Ser Met Val Glu Glu Thr Asn Gly 50 55 60 Val Gly Pro Glu Gly Lys Phe Leu Pro Leu Thr He Ser Pro Leu Leu 65 70 75 80 His Lys Thr Ser Arg Lys Ala Leu Thr Pro Thr Pro Ser Leu Ser Pro 85 90 95 Ala Asn He Ser Ser Met Pro Glu Phe Arg Asn Trp Ala Lys Gly Lys 100 105 110 He Asp Leu Asp Ser Asp Ser He Gly Trp Tyr Phe Lys Tyr Leu Asp 115 120 125 Pro Ala Gly Ala Thr Glu Ser Ala Arg Ala Val Gly Glu Tyr Ser Lys 130 135 140 He Pro Asp Gly Leu Val Lys Phe Ser Val Asp Ala Glu He Arg Glu 145 150 155 160 He Tyr Asn Glu Glu Cys Pro Val Val Thr Asp Val Ser Val Pro Leu 165 170 175 Asp Gly Arg Gin Trp Ser Leu Ser He Phe Ser Phe Pro Met Phe Arg 180 185 190 Thr Ala Tyr Val Ala Val Ala Asn Val Glu Asn Lys Glu Met Ser Leu 195 200 205 Asp Val Val Asn Asp Leu He Glu Trp Leu Asn Asn Leu Ala Asp Trp 210 215 220 Arg Tyr Val Val Asp Ser Glu Gin Trp He Asn Phe Thr Asn Asp Thr 225 230 235 240 Thr Tyr Tyr Val Arg He Arg Val Leu Arg Pro Thr Tyr Asp Val Pro 245 250 255 Asp Pro Thr Glu Gly Leu Val Arg Thr Val Ser Asp Tyr Arg Leu Thr 260 265 270 j SUBSTITUTE SHEET Tyr Lys Ala He Thr Cys Glu Ala Asn Met Pro Thr Leu Val Asp Gin 275 280 285 Gly Phe Trp He Gly Gly Gin Tyr Ala Leu Thr Pro Thr Ser Leu Pro 290 295 300 Gin Tyr Asp Val Ser Glu Ala Tyr Ala Leu His Thr Leu Thr Phe Ala 305 310 315 320 Arg Pro Ser Ser Ala Ala Ala Leu Ala Phe Val Trp Ala Gly Leu Pro 325 330 335 Gin Gly Gly Thr Ala Pro Ala Gly Thr Pro Ala Trp Glu Gin Ala Ser 340 345 350 Ser Gly Gly Tyr Leu Thr Trp Arg His Asn Gly Thr Thr Phe Pro Ala 355 360 365 Gly Ser Val Ser Tyr Val Leu Pro Glu Gly Phe Ala Leu Glu Arg Tyr 370 375 380 Asp Pro Asn Asp Gly Ser Trp Thr Asp Phe Ala Ser Ala Gly Asp Thr 385 390 395 400 Val Thr Phe Arg Gin Val Ala Val Asp Glu Val Val Val Thr Asn Asn 405 410 415 Pro Ala Gly Gly Gly Ser Ala Pro Thr Phe Thr Val Arg Val Pro Pro 420 425 430 Ser Asn Ala Tyr Thr Asn Thr Val Phe Arg Asn Thr Leu Leu Glu Thr 435 440 445 Arg Pro Ser Ser Arg Arg Leu Glu Leu Pro Met Pro Pro Ala Asp Phe 450 455 460 Gly Gin Thr Val Ala Asn Asn Pro Lys He Glu Gin Ser Leu Leu Lys 465 470 475 480 Glu Thr Leu Gly Cys Tyr Leu Val His Ser Lys Met Arg Asn Pro Val 485 490 495 Phe Gin Leu Thr Pro Ala Ser Ser Phe Gly Ala Val Ser Phe Asn Asn 500 505 510 Pro Gly Tyr Glu Arg Thr Arg Asp Leu Pro Asp Tyr Thr Gly He Arg 515 520 525 Asp Ser Phe Asp Gin Asn Met Ser Thr Ala Val Ala His Phe Arg Ser 530 535 540 Leu Ser His Ser Cys Ser He Val Thr Lys Thr Tyr Gin Gly Trp Glu 545 550 555 560 Gly Val Thr Asn Val Asn Thr Pro Phe Gly Gin Phe Ala His Ala Gly 565 570 575 Leu Leu Lys Asn Glu Glu He Leu Cys Leu Ala Asp Asp Leu Ala Thr 580 585 590 J SUBSTITUTE SHEET 1 Arg Leu Thr Gly Val Tyr Pro Ala Thr Asp Asn Phe Ala Ala Ala Val 595 600 605 Ser Ala Phe Ala Ala Asn Met Leu Ser Ser Val Leu Lys Ser Glu Ala 610 615 620 Thr Ser Ser He He Lys Ser Val Gly Glu Thr Ala Val Gly Ala Ala 625 630 635 640 Gin Ser Gly Leu Ala Lys Leu Pro Gly Leu Leu Met Ser Val Pro Gly 645 650 655 Lys He Ala Ala Arg Val Arg Ala Arg Arg Ala Arg Arg Arg Ala Ala 660 665 670 Arg Ala Asn 675 SUBSTITUTE SHEET

Claims: CLAIMS 1. An isolated small RNA virus capable of infecting insect species including Heliothis species. 2. The virus of claim 1 comprising a genome hybridizable with the nucleotide sequence of RNA 1 or RNA 2 as herein described. 3. The virus of claim 1 which comprises proteins which are capable of generating antibodies said antibodies being immunologically reactive with the large coat protein of HaSV as herein described. 4. The virus of claim 1 wherein said virus has a particle size of approximately 35 to 38 nm and comprises a genome with RNA of about 5.3 and 2.4 kb in length. 5. The virus of claim 4 wherein said particle comprises coat proteins of approximately 7 and 64 KDa. 6. The virus of claim 1 wherein said virus is HaSV or a mutant, variant or derivative thereof as herein described. 7. The virus of claim 6 wherein said virus comprises a nucleic acid sequence which is an encapsidation sequence, structure or signal with at least 50% nucleotide sequence identity to the corresponding nucleotide sequences of HaSV. 8. The virus of claim 6 wherein said virus comprises a nucleic acid sequence which encodes proteins with at least 60% amino acid sequence identity to the corresponding proteins or polypeptides of HaSV. 9. The virus of claim 6 wherein said virus comprises a nucleic acid sequence which has at least 50% nucleotide sequence identity to the portions SUBSTITUTE SHEET of the HaSV genome which encode: amino acid residues 401 to 600 of the replicase enzyme or amino acid residues 273 to 435 or 50 to 272 or 436 to the COOH terminus of the capsid protein. 10. The virus of claim 1, being that isolated in Example 1 or Example 2 herein or having a genome substantially similar to the virus isolated in Example 1 or Example 2. 11. An isolated nucleic acid molecule comprising a nucleic acid sequence hybridizable with RNA 1 or RNA 2 as herein described under low stringency conditions. 12. The molecule of claim 11 wherein said sequence is hybridizable under medium stringency conditions. 13. The molecule of claim 12 wherein said sequence is hybridizable under high stringency conditions. 14. The molecule of claim 11 wherein said sequence encodes P7, PI 6, PI 7, P64, P70, P71, Plla, Pllb, P14 or P187 or a mutant, variant or derivative thereof as herein described. 15. The molecule of claim 14 wherein said sequence encodes P7, P64, or P71 or a mutant, variant or derivative thereof as herein described. 16. The molecule of claim 11 capable of being used as a probe or primer for the nucleic acid sequence of RNA 1 or RNA 2, or mutants, variants or derivatives thereof, said molecule comprising nucleic acid sequences suitable for detection of, or replication of, RNA 1 or RNA 2, or portions thereof under appropriate conditions. SUBSTITUTE SHEET 17. The molecule of claim 16 capable of being used as one of a primer pair, wherein said primer is derived from a sequence of RNA 1 or RNA 2 which is located between 300 and 1500 bp from another sequence of RNA 1 or RNA 2 being the sequence of the other primer of said primer pair. 18. The molecule of claim 17 comprising the following sequences 5 ' GGGGGGAATTCATTTAGGTGACACTATAG . TTCTGCCTCCCCGGAC (called "HvRlSPSp" herein) 5' GGGGGGATCCTGGTATCCCAGGGGGGC (called "HvR13p" herein) 5' CCGGAAGCTΓGTTTTTCTTTCTTTACCA (called "Hr2cdna5" herein) 5' GGGGGATCCGATGGTATCCCGAGGGACGC TCAGCAGGTGGCATAGG (called "HvR23p" herein) AAATAATTTTGTTACTTTAGAAGGAGATATACATATGAGCGAGCGA GCACAC (called "HVPET65N" herein) AAATAATTrrGTTTAACC rAAGAAGGAGATCTACATΔTiαCTGGΔGI GGCGTCAC (called "HVPET63N" herein) ΓTGAΓTATΓTAΓATATGGGAGATGCTGGAGTG (called ΗVPET64N" herein) GTAGCGAACGTCGAGAA (called ΗVRNA2F3" herein) GGGGGATCCrTCAGTTGTCAGTGGCGGGGTAG (called ΗVP65C" herein) GGGGATCCCTAATTGGCACGAGCGGCGC (called ΗVP6C2" herein) j SUBSTITUTE SHEET AATTACATATGGCGGCCGCCGTTTCTGCC (called "HVP6MA" herein) AATTACATATGTTCGCGGCCGCCGTTTCT (called "HVP6MF" herein). 19. The molecule of claim 11 additionally comprising a ribozyme sequence. 20. A vector comprising the molecule of claim 11. 21. A vector comprising the molecule of claim 14. 22. A vector comprising the molecule of claim 15. 23. A vector comprising the molecule of claim 16. 24. A vector comprising the molecule of claim 17. 25. A vector comprising the molecule of claim 18. 26. A vector comprising the molecule of claim 11 capable of replication, expression and/or encapsidation in an animal, plant or bacterial cell. 27. A vector comprising the molecule of claim 11 capable of transferring said nucleic acid molecule to a plant cell. 28. The vector of claim 26 or claim 27 which comprises a ribozyme for facilitating rephcation, expression or encapsidation of the transcript. 29. The vector of claim 26 or claim 27 wherein having a ribozyme sequence selected from one of the following sequences 5'CCATCGATGCCGGACTGGTATCCCAGGGGG (called "HVRlCla" herein) SUBSTITUTE SHEET 5' CCATCGATGCCGGACTGGTATCCCGAGGGAC (called "5 , HVR2Cla" herein) 5' CCATCGATGATCCAGCCTCCTCGCGGCGCCGGATGGGCA (called "RZHDV1" herein) 5' GCTCTAGATCCATTCGCCATCCGAAGATGCCCATCCGGC (called "RZHDV2" herein) 5' CCATCGATTTATGCCGAGAAGGTAACCAGAGAAACACAC (called "RZHCl" herein) 5' GCTCTAGACCAGGTAATATACCACAACGTGTGTTTCTCT (called "RZHC2" herein) 30. The vector of claim 26 or claim 27 which comprises a promoter for facilitating expression said promoter selected from the group of the Drosophila promoters, heat shock promoters, baculovirus promoters, CMV promoters. 31. A vector of claim 20 comprising the plasmids pDHVRl, pDHVRlRZ, pDHVR2, pDHVR2RZ, pl7V71, pl7E71, pPH, pV71, pl7V64, pl7E64, pP64, pV64, pBacHVRl, pBacHVRlRZ, pBacHUR2, pBacHVR2RZ, pHSPRl, pHSPRlRZ, pHSPR2, pHSPR2RZ, pSRl(E3)A, pSRl(E3)B, pSR2A, pSR2B, pSX2P70, pSXR2P70, pSRP2B, pBHVRIB, pBHVR2B, pT7T2P64, pSR2P70, pT7T2P65, pT7T2P70, pT7T2-P71,pBSKSE3, pBSR15, pBSR25p, pSR25, phr236P70, phr235P65, pGemP63N, pGemP64N, pGemP65N, pP64N, pP65H, pTP6MA, pTP6MF, pTP17, pTP17delBB, pP656 or p70G as described herein. 32. A host cell comprising the vector of claim 20. 33. The host cell of claim 32 wherein said cell is an insect cell or a plant cell. SUBSTITUTE SHEET 34. An isolated protein or polypeptide preparation of the proteins or polypeptides derivable from the virus claimed in claim 1. 35. The preparation of claim 34 which comprises P7, P16, P17, P64, P70, P71, Plla, Pllb, P14 or P187 or mutants, variants or derivatives as described herein. 36. The preparation of claim 34 which comprises the large capsid protein or a mutant, derivative or variant thereof. 37. The preparation of claim 36 which comprises the gut binding domain of HaSV as herein described. 38. The preparation of claim 37 which comprises the variable regions of said gut binding domain. 39. An isolated antibody reactive with the protein or polypeptide preparation of claim 34. 40. An isolated antibody reactive with the protein or polypeptide preparation of claim 36. 41. An isolated antibody reactive with the protein or polypeptide preparation of claim 37. 42. An isolated antibody reactive with the protein or polypeptide preparation of claim 38. 43. The antibody of claim 39 wherein said antibody is a monoclonal antibody. SUBSTITUTE SHEET 44. The preparation of claim 34 which comprises assembled virus capsid proteins optional containing an insecticidally effective agent. 45. A recombinant insect virus vector comprising the nucleic acid molecule of claim 11. 46. The virus vector of claim 45 comprising material derived from baculovirus including NPV and GV, entomopoxvirus, cytoplasmic polyhedrosis virus. 47. The virus vector of claim 45 wherein said vector is capable of infecting insect species including Heliothis species. 48. The virus vector of claim 45 comprising one or more nucleic acid sequences which encode substances which are deleterious to insects. 49. A method of controlling insect attack in a plant comprising genetically manipulating said plant so that it is capable of expressing HaSV or mutants, derivatives or variants thereof, or an insecticidally effective portion of HaSV, mutants, derivatives or variants thereof and optionally other insecticidally effective agents such that insects feeding on the plant are deleteriously effected. 50. A transgenic plant resistant to insect attack comprising a genome or subgenome capable of expressing the molecule of claim 11. 51. The plant of claim 50 capable of expressing nucleic acid sequences encoding one or more substances that are deleterious to insects. 52. A preparation of HaSV or a mutant, variant or derivative thereof, or an insecticidally effective portion of said HaSV, or mutant, variant or derivatives SUBSTITUTE SHEET thereof, suitable for application to plants, said preparation capable of imparting an insect protective effect. 53. The plasmid vectors pT7T2b and pT7T2C as described herein. 54. A method of identifying HaSV or mutants, variants or derivatives thereof using the molecule of claim 11 or the antibodies of claim 39 to detect the presence of said HaSV in a sample. j SUBSTITUTE SHEET

Inventors: CHRISTIAN PETER DANIEL, GORDON KARL HEINRICH JULIUS, HANZLIK TERRY NELSON, CHRISTIAN, PETER, DANIEL, GORDON, KARL, HEINRICH, JULIUS, HANZLIK, TERRY, NELSON
Applicants: CHRISTIAN PETER DANIEL, COMMW SCIENT IND RES ORG, GORDON KARL HEINRICH JULIUS, HANZLIK TERRY NELSON, PACIFIC SEEDS PTY LTD, COMMONWEALTH SCIENTIFIC & INDUSTRIAL RESEARCH ORGA, PACIFIC SEEDS PTY. LTD.
Application year: 1993
Main classification: C12N7
IPC codes: C07K14, C12N7, C12N15, C12P21