Imidazole derivatives having anti-HIV activity

Abstract: A novel imidazole derivative of formula I: wherein: R¹ is hydrogen; C₁-C₁₀ alkyl; halogen; or C₆-C₁₂ aryl which is unsubstituted or may be substituted by C₁-C₁₀ alkyl, halogen, nitro, amino, hydroxy, C₁-C₁₀ alkoxy or aralkyloxy; R² is pyridyl; pyrimidinyl; pyridazinyl; pyrazinyl; imidazolyl; oxazolyl; or thiazolyl, each of which is unsubstituted or may be substituted by C₁-C₁₀ alkyl, halogen, nitro, amino, hydroxy, C₁-C₁₀ alkoxy or C₆-C₁₂-aryl-C₁-C₁₀-alkoxy; R³ is hydrogen; C₁-C₁₀ alkyl; C₆-C₁₂ aryl which is unsubstituted or may be substituted by C₁-C₁₀ alkyl, halogen, nitro, amino, hydroxy, C₁-C₁₀ alkoxy or C₆-C₁₂-aryl-C₁-C₁₀-alkoxy; C₆-C₁₂-aryl-C₁-C₁₀-alkxyl which is unsubstituted or may be substituted by C₁-C₁₀ alkyl, halogen, nitro, amino, hydroxy, C₁-C₁₀ alkoxy or C₆-C₁₂-aryl-C₁-C₁₀-alkoxy; or hydroxy(C₁-C₁₀)alkyl which is unsubstituted or may be substituted by C₆-C₁₂ aryl, C₁-C₁₀ alkyl, C₆-C₁₂-aryl-C₁-C₁₀-alkxyl, benzoyl, 4-nitrobenzoyl, 4-tert-butylbenzoyl, benzenesulfonyl, toluenesulfonyl, formyl, acetyl, propionyl, butyryl or valeryl; R⁴ is hydrogen; C₁-C₁₀ alkyl; halogen; benzoyl; 4-nitrobenzoyl; 4-tert-butylbenzoyl; benzenesulfonyl; toluenesulfonyl; formyl; acetyl; propionyl; butyryl; valeryl; hydroxy(C₁-C₁₀)alkyl which is unsubstituted or may be substituted by C₆-C₁₂ aryl, C₁-C₁₀ alkyl, C₆-C₁₂-aryl-C₁-C₁₀-alkxyl, benzoyl, 4-nitrobenzoyl, 4-tert-butylbenzoyl, benzenesulfonyl, toluenesulfonyl, formyl, acetyl, propionyl, butyryl or valeryl; carboxyl; methoxycarbonyl; ethoxycarbonyl; tert-butoxycarbonyl; benzyloxycarbonyl; hydroxy; C₆-C₁₂ aryl; or C₆-C₁₂ arylthio; X is S, SO, SO₂, CH₂, or Se; and n is an integer of 1 to 3, or a pharmaceutically acceptable salt thereof, said derivative having anti-HIV activity and being useful for the treatment of HIV infections.

Description: The present invention relates to novel imidazole derivatives, more particularly it relates to novel imidazole derivatives having anti-retrovirus activity and pharmaceutical compositions effective for the treatment of retrovirus infections such as human immunodeficiency syndromes. AIDS (acquired immunodeficiency syndrome) is caused by a member of retrovirus, human immunodeficiency virus (HIV). In spite of great efforts made by researchers there have been no effective methods for treating AIDS so far, and the disease is becoming a more and more serious problem. It has been reported that pyrimidine nucleoside compounds can be useful anti-retrovirus agents because of the inhibitory activity against the reverse transcription of RNA of retroviruses and many compounds have been synthesized. Among them, azidodeoxythymidine (AZT) has been practically used for treating AIDS. AZT, however, cannot be used broadly due to its high toxicity. Accordingly, the development of anti-HIV compounds which are highly effective and less toxic has been demanded strongly. It has been reported that acyclovir, an acyclonucleoside derivative, has an inhibitory effect on herpes virus and hence a lot of acyclonucleosides with various side chains were synthesized [J. Heterocyclic Chem 23 , 289 (1986)]. The literature failed to mention about activities of such compounds against retrovirus. The present inventors have made an intensive investigation for the purpose of developing compounds having anti-retrovirus activity and being useful for the treatment of AIDS; they found that certain imidazole derivatives have such an activity and can prevent HIV infection. Thus, the present invention provides compounds of the formula I: wherein R 1 is hydrogen, alkyl, halogen, or optionally substituted aryl; R 2 is optionally substituted hetero ring group; R 3 is hydrogen, alkyl, optionally substituted aryl, optionally substituted aralkyl, or optionally substituted hydroxyalkyl; R 4 is hydrogen, alkyl, halogen, acyl, optionally substituted hydroxyalkyl, optionally esterified or amidated carboxyl group, hydroxy group, aryl or arylthio; X is S, SO, SO 2 , CH 2 , or Se; n is an integer of 1 to 3, or a pharmaceutically acceptable salt thereof. For purposes of the present invention, as disclosed and claimed herein, the following terms are defined below. The term "alkyl" means a straight or branched saturated hydrocarbon radical having one to ten carbon atoms, preferably, a straight or branched alkyl radical having one to six carbon atoms. Examples of alkyl include methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, s-butyl, t-butyl, n-pentyl, i-pentyl, neopentyl, s-pentyl, t-pentyl, n-hexyl, neohexyl, i-hexyl, s-hexyl, t-hexyl and the like. For purposes of the present invention, a straight or branched lower alkyl having one to four carbon atoms such as methyl, ethyl, n-propyl, isopropyl or t-butyl is particularly preferred. The term "halogen" means fluorine, chlorine, bromine and iodine. The term "acyl" means an aromatic or aliphatic acyl group. Examples of aromatic acyl groups are benzoyl, 4-nitrobenzoyl, 4-tert-butylbenzoyl, benzenesulfonyl, toluenesulfonyl and the like. Examples of aliphatic acyl groups are formyl, acetyl, propionyl, butyryl, valeryl and the like. Aliphatic acyl group is preferred, and formyl is the most preferable group. The term "aryl" means an aromatic ring group having six to twelve carbon atoms. Examples of aryl group include phenyl, tolyl, xylyl, biphenyl, naphthyl and the like, with the preference of phenyl. In the definition of "optionally substituted aryl", examples of the substituents include alkyl, halogen, nitro, amino, hydroxy, alkoxy, and aralkyloxy as defined above, with the preference of methyl, fluorine, and chlorine. The term "hydroxyalkyl" means those derived from an alkyl group as defined above. Preferred hydroxyalkyl groups are hydroxymethyl and hydroxyethyl. In the definition of "optionally substituted hydroxyalkyl", substituted hydroxyalkyl means those which are derived by subustituting an alkyl, aryl, aralkyl or acyl for the hydrogen of the hydroxy group, namely, it includes alkyloxyalkyl, aryloxyalkyl, aralkyloxyalkyl, acyloxyalkyl and the like. Especially, benzyloxyalkyls are preferable. Examples of "optionally esterified carboxyl group" include carboxyl group, methoxycarbonyl group, ethoxycarbonyl, tert-butoxycarbonyl, benzyloxycarbonyl and the like, with the preference of ethoxycarbonyl. Salts of compounds of the formula (I) are pharmaceutically acceptable salts formed between a compound (I) and an inorganic acid such as hydrochloric acid, nitric acid, phosphoric acid, sulfuric acid, hydrobromic acid, hydroiodic acid, nitrous acid, phosphorous acid or the like; or a non-toxic organic acid such as aliphatic mono-or dicarboxylic acid, phenyl-substituted alkanoic acid, hydroxy-mono- or hydroxy-di-alkanoic acid, aromatic acid, aliphatic or aromatic sulfonic acid or the like. Examples of pharmaceutically acceptable acid addition salts include sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, nitrate, phosphate, secondary phosphate, primary phosphate, metaphosphate, pyrophosphate, hydrochloride, hydrobromide, hydroiodide, hydrofluoride, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caproate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, mandelate, butyn-1,4-dioate, hexyn-1,6-dioate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, phthalate, terephthalate, benzenesulfonate, toluenesulfonate, chlorobenzenesulfonate, xylenesulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, β-hydroxybutyrlate, glycolate, malate, tartrate, methanesulfonate, propanesulfonate, naphthalene-1-sulfonate, naphthalene-2-sulfonate and the like. For purposes of the invention, inorganic salts are preferred, and hydrochloride and nitrate are particularly preferable. All the compounds (I) as defined above commonly possess the objective anti-retrovirus activity and are useful for the treatment of AIDS. There are certain preferable compounds which have higher anti-HIV activity, e.g., compounds (I) wherein R 1 is hydrogen, C 1 -C 3 alkyl, in particular, methyl, ethyl or propyl, halogen, in particular, bromine, or phenyl; R 2 is C 1 - C 4 alkyl, in particular, butyl, phenyl, phenyl substituted by halogen or lower alkyl, in particular, dimethylphenyl; R 3 is C 1 - C 3 alkyl, in particular, ethyl, benzyl, or hydroxyalkyl, in particular, hydroxyethyl; R 4 is hydrogen, C 1 -C 3 alkyl, in particular, methyl or ethyl, halogen , in particular, bromine, formyl, hydroxymethyl, ethoxycarbonyl, carbamoyl, benzyloxymethyl, hydroxyl or phenylthio group; and X is S or CH 2 are preferred for purposes of the invention. As can be understood easily from the formula I, the compounds (I) of the present invention can be in two position isomers in which a substituent of the formula -CH 2 -O-R 3 is attached to either of nitrogen atoms in the imidazole ring. The preparation of the compounds (I) of the present invention can be carried out by any of known methods, for example, those shown by the synthetic routes A, B, C, D, E, F, G, H, and/or I below. In the reaction scheme above, X' means S or Se and Y means halogen. The reactions in the synthetic route A can be carried out according to the teachings shown at every step by using an appropriate imidazole derivative, e.g. 4-substituted-imidazole as the starting material. Each reaction can be carried out by employing conditions known to those skilled in the art. For example, in the first step, an iodine atom is introduced at the 5-position of imidazole ring in the presence of a base in a solvent such as methylene chloride or the like. In the second step, the resultant imidazolyl iodide is added to a mixture of sodium hydride and a solution of thiophenol (or selenophenol) in an appropriate solvent such as dry dimethylformamide to introduce a phenylthio (or phenylseleno) group at the 5-position of imidazole ring. In the third step, a desired substituent, -(CH 2 ) n -O-R 3 , is introduced at a nitrogen atom on imidazole ring. This reaction tends to give a mixture of position isomers as a product, in which the group -(CH 2 ) n -O-R 3 is attached to either nitrogen atoms at 1- or 3-positions on imidazole ring. The isomer having the substituent at the desired position can be isolated by any of conventional methods such as silica gel chromatography. In the fourth step, the imidazole derivatives obtained in steps 1 to 3 above are converted into the objective compound (I). Thus, the hydrogen at the 2-position of imidazole ring is replaced by a desired substituent in the presence of an electrophilic reagent known per se in the art, for instance, n-butyllithium. In the reaction scheme above, X' and Y are the same as defined in the Route A. The process is carried out substantially in the same manner as in Route A, except that the substituent R 4 is introduced before the intoroduction of -CH 2 -O-R 3 to the imidazole ring. In the reactions scheme above, X' and Y are the same as defined in Routes A and B. This process is carried out substantially in the same manner as in Route A or B, using 2,4-substituted-imidazoles as starting materials. Most of the starting materials are known compounds or, if necessary, they may be readily prepared by conventional reactions. In the reactions scheme above, R 1 , R 2 , R 3 , R 4 , and X are the same as defined before. The starting material A, an α-acylamino-α-substituted-ketone, is refluxed in an organic acid solvent such as actic acid in the presence of ammonia or the salts with organic acids or inorganic acids to give an objective 2,4,5-substituted imidazole. The subsequent reaction may be carried out according to the manner in Route A, B, or C. The starting material A can be prepared, for example, through either of the following routes. 1) X = CH 2 Amino acid is halogenated with thionyl halide or phosphorus oxychloride and then refluxed with alkanol to give the ester, which is reacted with an acid anhydride or an activated oganic acid such as acid halide in the presence of a base to give an N-acylamino acid ester. This is treated with a carbanion reagent as Grignard reagents or organic lithium reagent in an aprotic solvent to give the starting material A. 2) X = CH 2 Acylaminomalonic acid diester is in the presence of a base alkylated with a substituted methyl halide in an aprotic solvent to give α-aminoacyl-α-substituted malonic acid diester, which is hydrolyzed and decarboxylated, followed by esterification. The resulting ester is treated in the same manner as aforementioned to give the starting material A. 3) X = S, SO, SO 2 or Se A substituted (XR 2 ) acetic acid ester is treated with azide transfer reagent in the presence of a base in an aprotic solvent at a temperature of from -100°C to room temperature. The azide transfer agent inchicles, for example, benzenesulfonylazide, 2,4,6-trimethylbenzenesulfonylazide, diphenylphosphorylazide and the like. The reaction mixture is sujected to the same reaction as shown above to give the corresponding ketone. The azide group of the ketone is reduced and acylated to give the starting material A. Compounds (I) wherein X is SO or SO 2 can be readily obtained by oxidation of the corresponding sulfides. The oxidation is carried out in an appropriate solvent such as methylene chloride, acetonitrile and the like solvent with one or two mole of an appropriate oxidizing agent such as sodium periodate, m-chloroperbenzoic acid, hydrogen peroxide, sodium dichromate, potassium permanganate, chromic acid and the like. Thus obtained compounds (I) in the synthetic route A, B, C, D or E above may be further modified according to the synthetic route F, G, H, or I as described below to yield desired imidazole derivatives. Each reaction in the synthetic routes as noted above are well known to a person skilled in the art and can be effected by applying the well known reaction conditions. In the working Examples as is hereinafter described, specific procedures for each synthetic routes are given and one of skilled in the art can obtain the compounds of the present invention by employing the same conditions as those used in the Examples or others. For instance, illustrative procedures for each synthetic routes can be seen in the following Examples. Although the present invention is by no means restricted to the production of a compound (I), there is a certain preferable synthetic route for each compound depending on the definition of "X" in the formula (I) as shown below. Preferable synthetic routes of compounds (I) Synthetic route X of formula (I) A, B, C S, Se D S, SO, SO 2 , Se, CH 2 F S, Se, CH 2 G, H, I S, SO, SO 2 , Se, CH 2 As can be seen from the results obtained by experiments described below, the novel compounds (I) of the invention exhibit an anti-retrovirus activity in vitro, can be used in the prevention and/or treatment of HIV (HTLV-IIIB strain) infection. The compounds of the present invention can be orally or parenterally administered. For oral administration, the compounds of the present invention can be used in any formulations in conventional forms such as solid formulations including tablets, powders, granules, capsules and the like; aqueous solutions; oily suspensions; and liquid formulations including syrups, elixirs and the like. For parenteral administration, the compounds of the present invention can be formulated as an aqueous or oily suspension injections. The formulations of the invention can be prepared by using conventional adjuvant such as excipients, binders, lubricants, aqueous solvents, oily solvents, emulsifiers, suspending agents or the like. The formulations may comprise further supplementals such as preservatives, stabilizers or the like. Appropriate daily dosage of the compounds or salts thereof of the present invention varies depending on various factors such as administration route, age, body weight, conditions and symptoms of the patient to be treated. Generally, the daily dose may be between 0.05 - 1500 mg, preferably 0.1 - 500 mg on oral administration, and 0.01 - 1000 mg, preferably 0.05 - 300 mg on parenteral administration, said daily dose being administered in 1 to 5 divisions. The following example is set forth to further describe the invention but is in no way meant to be construed as limiting the scope thereof. Reference Example 1 5-Benzyl-1-benzyloxymethyl-4-isopropyl-2-methylimidazole (I-42) (1) N-acetylphenylalanine methyl ester 4 To a solution of 2.73 g of L-phenylalanine methyl ester hydrochloride (12.7 mmol) in 5.00 g of pyridine (63.2 mmol) is dropwise added 12.90 g of acetic anhydride (126.0 mmol) under ice cooling. The mixture is allowed to stand for 2 hours and concentrated in vacuo. The residue is extracted with ethyl acetate. The extract is washed with 5% hydrochloric acid, saturated aqueous sodium bicarbonate and saturated brine in order, dried over sodium sulfate, filtered and concentrated in vacuo to give 2.34 g of the objective N-acetylphenylalanine methyl ester 4 . Yield, 83.6%. I-42: 1 H-NMR(CDCl 3 -TMS)δppm: 1.99(s,3H), 3.13(m,2H), 3.73(s,3H), 4.89(m,1H), 5.89(br,1H), 7.06-7.33(m,5H) Elemental analysis(C 12 H 15 NO 3 ) Calcd.(%);C,65.14:H,6.83:N,6.33 Found (%);C,65.12:H,6.80:N,6.31 (2) 4 -Acetylamino-2-methyl-5-phenyl-3-pentanone 5 A solution of 2.30 g of N-acetylphenylalanine methyl ester (10.40 mmol) in 15 ml of tetrahydrofuran is chilled at -60°C with dry ice/acetone bath. To the mixture is added dropwise 10.40 ml of 2 M solution of isopropylmagnesium chloride in tetrahydrofuran (20.8 mmol) with stirring and the mixture allowed to stand for 15 minutes and returned to room temperature. The mixture was stirred for 3 hours. To the mixture is added saturated aqueous ammonium chloride under ice cooling, followed by the addition of 5% hydrochloric acid and the mixture extracted with ethyl acetate. The extract is washed with saturated brine, dried over sodium sulfate, filtered and the filtrate concentrated in vacuo. The resulting crude product is chromatographed on a column of silica gel, eluting with methylene chloride and ethyl acetate (4:1) to give 1.40 g of the objective 4-acetylamino-2-methyl-5-phenyl-3-pentanone 5. Yield, 57.7%. 5: 1 H-NMR(CDCl 3 -TMS)δppm: 1.00(d,J=6.6Hz,3H), 1.09(d,J=7.2Hz,3H), 1.97(s,3H), 2.68(m,lH), 3.04(m,2H), 5.06(m,lH), 6.07(br,1H), 7.08-7.34(m,5H) Elemental analysis(C 14 H 19 NO 2 0.1H 2 O) Calcd.(%);C,71.52:H,8.23:N,5.96 Found (%);C,71.62:H,8.08:N,6.03 (3) 5-Benzyl-4-isopropyl-2-methylimidazole 6 To a solution of 1.00 g of 4-acetylamino-2-methyl-5-phenyl-3-pentanone (4.29 mmol) in 20 ml of acetic acid is added 5.00 g of ammonium acetate (64.9 mmol), and the resultant mixture is heated under reflux. One hour later, the reaction mixture is concentrated in vacuo, neutralized with aqueous sodium bicarbonate and extracted with ethyl acetate. The extract is washed with saturated brine, dried over sodium sulfate, filtered and the filtrate is concentrated. The crude product is recrystallized from ether/n-hexane to give 0.90 mg of the objective 5-benzyl-4-isopropyl-2-methylimidazole 6. Yield, 97.5%. 6: 1 H-NMR(CDCl 3 -TMS)δppm: 1,23(d,J=7.0Hz,6H), 2.29(m,1H), 3.86(s,2H), 7.13-7.31(m,5H) Elemental analysis(C 14 H 18 N 2 ) Calcd.(%);C,78.46:H,8.47:N,13.07 Found (%);C,78.20:H,8.50:N,12.89 (4) 5 -Benzyl-1-benzyloxymethyl-4-isopropyl-2-methylimidazole (I-42) To a solution of 980 mg of 5-benzyl-4-isopropyl-2-methylimidazole (4.58 mmol) in 9.8 ml of dry dimethylformamide is added 238 mg of 60% oil suspension of sodium hydride (5.95 mmol) under ice cooling. Ten minutes later, 935 mg of benzyloxymethyl chloride (5.97 mmol) is dropwise added. One hour later, the reaction mixture is poured onto ice water and extracted with ethyl acetate. The extract is washed with saturated brine, dried over sodium sulfate, filtered and the filtrate is concentrated in vacuo. The crude product is chromatographed on a column of silica gel, eluting with ethyl acetate and n-hexane (1:1) to give 820 mg of the objective 5-benzyl-1-benzyloxymethyl-4-isopropyl-2-methylimidazole (I-42). Yield, 54.17%. I-42 : 1 H-NMR(CDCl 3 -TMS)δppm: 1.28(d,J=6.6Hz,6H), 2.38(s,3H), 2.93(m,1H), 3.96(s,2H), 4.32(s,2H), 4.96(s,2H), 7.01-7.39(m,10H) Elemental analysis(C 22 H 26 N 2 O 0.8H 2 O) Calcd.(%);C,75.74:H,7.97:N,8.03 Found (%);C,75.78:H,7.75:N,8.00 Examples 1-4 In Examples I-4, desired compounds (I-1)-(I-4) as shown in the following Table I were prepared according to the synthetic route D in the same manner as described in Reference Example I above. I-1: 1 H-NMR (CDCl 3 -TMS)ppm: 1.05(t,J=7.0Hz,3H), 1.27 (d,J=7.2Hz,6H), 2.44(s,3H), 2.88(m,1H), 3.31(q,J=7.0Hz,2H), 3.99(s,2H), 4.92(s,2H), 7.04(d,J=6.2Hz,2H), 8.50(d,J=5.8Hz,2H) I-2: 1 H-NMR(CDCl 3 -TMS)ppm: 1.06(t,J=7.0Hz,3H), 1.28 (d,J=6.8Hz,6H), 2.44(s,3H), 2.90(m,1H), 3.31(q,J=7.0Hz,2H), 4.00(s,2H), 4.94(s,2H), 7.18-7.74(m,2H), 8.39-8.53(m,2H) I-3: 1 H-NMR (CDCl 3 -TMS)ppm: 1.24(d,J=7.4Hz,6H), 2.36(s,3H), 3.05(m,1H), 3.95(s,2H), 4.51(s,2H), 5.22(s,2H), 7.15-7.60(m,7H), 8.39-8.52(m,2H) I-4: 1 H-NMR(CDCl 3 -TMS)ppm: 1.05(t,J=7.0Hz,3H), 1.25(dJ=7.0Hz,6H), 2.42(s,3H), 2.93(m,1H), 3.33(q,J=7.0Hz,2H), 4.16(s,2H), 5.08(s,2H), 6.95-7.62(m,3H), 8.50-8.55(m,1H) Elemental analysis(C 16 H 23 N 3 O 0.3H 2 O) Calcd.:C,68.93:H,8.53:N,15.07 Found :C,68.83:H,8.35:N,15.05 Compounds (I) obtained in the foregoing Examples are evaluated as to the anti-retrovirus activity and the cytotoxicity. Examples 5 - 40 In the same manner as described above, Compounds I-5 to I-40 are prepared. Experiment 1 Infection Inhibitory Test Anti-HIV activity and cytotoxicity are tested in the following manners. 1. Antivirus Activity (1) A human T cell MOLT-4 clone 8, a strain continuously infected with HIV (HTLV-IIIB strain), was incubated in RPMI-1640 medium containing 10% bovine fetus serum and the supernatant was isolated by filtration. After the determination of virus titer, the supernatant was stored at -80°C. A compound to be tested was diluted with the same medium to a given concentration and poured into 96-well microtiter plates by 100 µl/well. To the well is added 50 µl (2.5 x 10 4 cells) MT-4 cell suspensions, followed by the addition of 50 µl [600 pfu (plaque forming unit)] HIV-containing supernatant previously diluted with the same medium. (2) After 5-day-incubation at 37°C in a carbon dioxide incubator, 30 µl MTT {3-(4,5-diemthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), 5 mg/ml in PBS} was added to each well, and the incubation was continued for another 1 hour, during which surviving cells reduce MTT to precipitate folmazan. The resultant folmazan was eluted by replacing 150 µl supernatant of a culture in each well by 150 µl solution comprising 10% triton X - 100 and isopropanol containing 0.4 v/v % HC1 and shaking by means of a plate mixer. The resultant folmazan was assayed at 0D 540 nm, and the result was evaluated by comparison with that obtained in a control test. IC 50 is defined as a concentration of the compound (I) that gives a 50% inhibition against viral cytotoxicity. The results are summarized in Table 6. Compound No. IC 50 (µg/ml) I-2 0.5 AZT 0.005-0.01 TIBO(R-82913) 0.13 -0.25 2. Cytotoxicity Cytotoxicity of a compound (I) was evaluated in a similar manner as described in 1 above except that 50 µl of the same medium is added to each well in place of the HIV-containing supernatant (viral solution). CC 50 is defined as a concentration of the compound (I) that gives 50% cytotoxicity. Results are summarized in Table 1 below. As is clear from the results of Tables 6 and 7, the compounds of the present invention or salts thereof possess anti-HIV activity, whereby the compounds of the invention can be used for the treatment of HIV virus infections such as AIDS or the like but also the development of derivatives improved pharmacological properties.

Claims: A compound of the formula I: wherein: R 1 is hydrogen; C 1 -C 10 alkyl; halogen; or C 6 -C 12 aryl which is unsubstituted or may be substituted by C 1 -C 10 alkyl, halogen, nitro, amino, hydroxy, C 1 -C 10 alkoxy or araikyloxy; R 2 is pyridyl; pyrimidinyl; pyridazinyl; pyrazinyl; imidazolyl; oxazolyl; or thiazolyl, each of which is unsubstituted or may be substituted by C 1 -C 10 alkyl, halogen, nitro, amino, hydroxy, C 1 -C 10 alkoxy or C 6 -C 12 -aryl-C 1 -C 10 -alkoxy; R 3 is hydrogen; C 1 -C 10 alkyl; C 6 -C 12 aryl which is . unsubstituted or may be substituted by C 1 -C 10 alkyl, halogen, nitro, amino, hydroxy, C 1 -C 10 alkoxy or C 6 -C 12 -aryl-C 1 -C 10 -alkoxy; C 6 -C 12 -aryl-C 1 -C 10 -alkyl which is unsubstituted or may be substituted by C 1 -C 10 alkyl, halogen, nitro, amino, hydroxy, C 1 -C 10 alkoxy or C 6 -C 12 -aryl-C 1 -C 10 -alkoxy; or hydroxy(C 1 -C 10 )alkyl which is unsubstituted or may be substituted by C 6 -C 12 aryl, C 1 -C 10 alkyl, C 6 -C 12 -aryl-C 1 -C 10 -alkyl, benzoyl, 4-nitrobenzoyl, 4-tert-butylbenzoyl, benzenesulfonyl, toluenesulfonyi, formyl, acetyl, propionyl, butyryl or valeryl; R 4 is hydrogen; C 1 -C 10 alkyl; halogen; benzoyl; 4-nitrobenzoyl; 4-tert-butylbenzoyl; benzenesulfonyl; toluenesulfonyl; formyl; acetyl; propionyl; butyryl; valeryl; hydroxy(C 1 -C 10 )alkyl which is unsubstituted or may be substituted by C 6 -C 12 aryl, C 1 -C 10 alkyl, C 6 -C 12 -aryl-C 1 -C 10 -alkxyl, benzoyl, 4-nitrobenzoyl, 4-tert-butylbenzoyl, benzenesulfonyl, toluenesulfonyl, formyl, acetyl, propionyl, butyryl or valeryl; carboxyl; methoxycarbonyl; ethoxycarbonyl; tert-butoxycarbonyl; benzyloxycarbonyl; hydroxy; C 6 -C 12 aryl; or C 6 -C 12 arylthio; x is S, SO, SO 2 , CH 2 , or Se; and n is an integer of 1 to 3, or a pharmaceutically acceptable salt thereof. A compound according to claim 1, wherein: R 1 is hydrogen; C 1 -C 4 alkyl; halogen; or phenyl; R 2 is pyridyl; R 3 is C 1 -C 4 alkyl; benzyl; or hydroxy(C 1 -C 10 )alkyl; R 4 is hydrogen; C 1 -C 4 alkyl; halogen; formyl; hydroxymethyl; hydroxymethyl substituted by C 6 -C 12 aryl, C 1 -C 10 alkyl, C 6 -C 12 -aryl-C 1 -C 10 -alkyl, benzoyl, 4-nitrobenzoyl, 4-tert-butylbenzoyl, benzenesulfonyl, toluenesulfonyl, formyl, acetyl, propionyl, butyryl or valeryl; carboxyl; methoxycarbonyl; ethoxycarbonyl; tert-butoxycarbonyl; benzyloxycarbonyl, hydroxy or phenylthio; X is S or CH 2 ; and n is an integer of 1. A pharmaceutical composition comprising an effective amount of a compound of Cliam 1 or Claim 2 in association with a pharmaceutically acceptable, substantially nontoxic carrier or excipient. An antivirus agent which comprises an effective amount of a compound of Claim 1 or Claim 2 together with pharmaceutically acceptable carriers therefor. The use of a compound of Claim 1 in the preparation of a pharmaceutical formulation for the treatment of a retrovirus infection, such as human immunodeficiency syndrome.

Inventors: FUJIWARA TAMIO, MATSUMOTO HIROSHI, OGATA MASARU, SATO AKIHIKO, SUGIMOTO HIROHIKO, SUGITA KEN-ICHI, FUJIWARA, TAMIO, MATSUMOTO, HIROSHI, OGATA, MASARU, SATO, AKIHIKO, SUGIMOTO, HIROHIKO, SUGITA, KEN-ICHI
Applicants: SHIONOGI & CO, SHIONOGI & CO., LTD.
Application year: 1993
Main classification: C07D401
IPC codes: C07D233, A61K31, C07D403, C07D401, C07D413, C07D417, A61P31