METHOD OF OBTAINING ANTIALLERGIC COMPONENTS FROM THE HOT-BED FIELD AGARIC BODY WITH EXCLUSION OF PATHOGENIC ORGANISM

Abstract: The present invention relates to a specific method of champignon fungus treatment by which a dry mass is obtained including a substance which provides for an outstanding antiallergic effect. Said method is characterized by step of disinfection carried out with proper disinfection means and preceding the steps of drying and cutting of said champignon mass, these steps being followed by the step of sterilization by ionization comprising the use of a cobalt charge (bomb) which is carried out after said mass has been formed into a final product.

Description: L3ETH0D OF OBTAINING ANTIALLERGIC COMPOIJEIJTS PROM THE HOT¬ BED FIELD AGARIC ' BODY WITH EXCLUSION OP PATHOGENIC ORGAI-ΪISM The Pield of Invention The present invention relates to a method of treating the hot-bed field agaric body belonging to- genus Agaricus hortensis capable of obtaining components providing for an antiallergic effect and being free of a contamination by pa¬ thogenic organises Description of the Prior Art ' The hot-bed field agaric known also as champignon in¬ cludes, according to a research made by French physician Da- guin, a substance effective against the allergic diseases and especially, against the dermatic ones. The same physi¬ cian has published in 1944 at that time a surprising finding based on the statistical data collected from 36 clinical ca¬ ses that the dry extract from said champignon fungi includes a very effective substance bringing about the healing of va¬ rious allergic diseaseso After consumption of a greater quan¬ tity of champignons an outstanding decline in clinic&l symptoms of dermatic diseases and of bronchial allergies has accured. In t -CSPR this particular problem has been studied by a me¬ dical team of Marie Apetauer, MD and Jaroslav Vesely, MD. The results of that study were published in Cs. Dermatology, 1975 Εo . 6, page 373-377 and later repeatedly in 1961, No. 5, pa¬ ge 329-334. In the period between 1957 and 1 61 both physi¬ cians were occupied with testing the new therapy on some al¬ lergic diseases with use of a dry champignon substance of ge¬ nus Agaricus campe3ter hortensis Cooke and in result, they confirmed an outstanding antiallergic effect in cases of derma¬ tic allergies both acute and chronic ones. The named authors recommended, with respect to the applied therapy using dry substance obtained from the champignon fungi, and on the gro¬ und of the clinically verified cases resisting, so far, to any - Z - usual antiallergetic therapy, to introducd the new drug into production. This idea, however, has not been accepted because the drug obtained by the method known at that could not comply with the proper hygienical regulationso Even at present, it is still not known which principle brings about the effect based on use of the champignon n¬ gi. It is considered that the curative effect related to the allergic diseases may be dependent of some ferments inclu¬ ded in the champignon substance and which, so far, could not be separated therefrom. However, according to present knowledge there exists relation between the way of fungus preparation and the healing effect and especially, it is known that a tretment at high temperatures could destroy or at le¬ ast degrade the influence of the effective ferments. At pre¬ sent, there exists obvious increase in number of allergical disseases and especially among the infantile population. These disseanses are characterized by various clinical symp» to s, however, they are mostly of a dermatic origin. The me¬ dicaments applied in such cases are neither indifferent nor without secondary effects. In addition, such medicaments are to be applied chronically and often for whole life which could influence the complete state of health. In co pa±'isi- on with the conventional medicaments the present drug obtai¬ ned from champignon fungi provides for a reliable antialler¬ gic effect without secondary indications. From reasons stated above said drug is occasionally be¬ ing introduced on market by the popular healers. It is a pity that this happens with a product which resulted from an un¬ satisfactory treatment and could cause the risks of a secon¬ dary infection for the patients. This is because of the pre¬ sence of pathogenetic microorganisms which occure in intrea- ted champignon fungi as in great quantities as in many genera. The champignon bodies, similarly as the bodies of other fungi of higher geni remain, in spi e of the drying routine, stron¬ gly contaminated with microorganisms. In case of fungus plan- ting in monocultures that contamination is far greater than at natural champignon cultures because the artifici¬ al planting requires application of a substrate fertilizer including the horse manure. Therefore, the powder made *_ from industrially cultivated champignon fungi is contami¬ nated with large quantities of microbe cultures as for the instance Escherichia coli, Ξnterobacteria aerogenes, Staphylococcus epidermis, Staphylococcus aureus, Proteus, microbes of the genus Clostridiu , Bacillus and a number of moulds. Therefore, the drug prepared and applied under above described conditions could not comply with regulati¬ ons concerning the hygienical, food productive and medical requirements. Summary of the Invention The main problem is seen in disinfecting and sterili¬ zing the dry champignon mass in such a wav and by such means which would retain the effectiveness thereof. With respect to the fact that the effective substance itself, included obviously in the champignon fungus, is yet not known and that the present knowledge of the proper therapy capable of effe¬ ctive healing of the allergical symptoms is considerably un- complete, there is the way in devoloping an utmost treatment and preparation of the fungus body mass belonging to the ge¬ nus Agaricus and to the hybrides thereof. Consequently, the object of the present invention com¬ prises a specific method of champignon body treatment in or¬ der to preserve the same as an usual and harmless eatable component and to retain its effective substances bringing about an outstanding antiallergic effect. According to this method of said champignon body treatment the dry champignon * mass being obtained, for example, by drying it at tempera¬ tures not exceeding 60 C or by lyophilization and by pulver- ization thereof is, subsequently, being disinfected by means of the solutions of chlora ine, sorbic acid, ethanol, pera- cetic acid, benzole acid and by soluble salts thereof, res¬ pectively. Before the next step the fungus mass is being rinsed with a drinking water and thereafter, sterilized by gas, as for the instance, by ethylen-oxid or gluta- raldehyd within a closed environment« Subsequently, the fungus mass is cleaned with a stream of sterile air and. thereafter sterilized with ionization dose od 0,1 to 15 kGy. As a radiation source a cobalt charge of 60Co-radi¬ ator is usedo As a result, there is obtained a substance being com¬ pletely harmless both from the hygienical and the medical point of view. At the production and the treatment of food, eatables and pharmaceutical goods it is necessary to respect regulations and norms issued by the -Iiniεtry ot health and Ministry of agriculture and alimentation, namely the Provision of the HZd CSSR Ho, 13 from 1955 con¬ cerning the hygienical and antiepidemical conditions at the production of eatables, Provision No. 46 concerning the hygienical conditions of working environment, Norm No S6 2000 relating to the industrially produced pharmaceu¬ tical goods, State -form No. 86 6002 concerning the medical drugs and State Norm No. 56 01 00 concerning microbiolo¬ gical testing of eatables, articles of a common use and of the food production environment. Examples of Elaboration of the Invention Example 1 ' The gathered champignon f ngi were purified by a me¬ chanical way and before the cutting thereof they have been disinfected in order to prevent leaching and subsequent loss of the substances providing for an antiallergic effect. Said disinfection was performed with 1,5 solution of chlor- amide for the period of 15 minutes. Thereafter, the fungi were removed from said chlora ine solution and rinsed with a drink water in order to wash off any residua of chlorine. After the dripping has taken place the fungi were cut and dried in a drier by steadily streaming air at temperature of 40 °Cβ Finally, the dry mass was pulverized an formed for the purpose of application per os. In addition, it ellowed the sterilization of the product by ionization from a cobalt charge emitting the dose of 10 kGy. Example 2 The mechanically purifie champignon fungi were dis¬ infected by 1 % solution of sorbic for the period of 10 minuteso Subsequently, the fungi were rinsed with a drink water and thereafter cut, drie and pulverized. For purpose of inal sterilization by ionization a cobalt charge emitting dose of 3 kGy has been used. Example 3 In this case it wad used a different way of disin- feciton. The purified fungi were treated with 40 ■ _. solu¬ tion of ethanol . for the period of 10 minutes. Further steps which followed corresponded with previous example. Industrial Utilization of the Invention By the steps of disinfection and sterilization of the fungus mass the pathogenic substances therein are neutra¬ lized, and in addition, a leaching of antiallergic sub¬ stances is prevented whereby an effective, hygienically and sanitary harmless substance is obtained being suitab¬ le for treatment of allergic difficulties. The same sub¬ stance would be applicable also externally for dermatolo- gical purposes as a cosmetic preparation with antialler¬ gic affect.

Claims: PA T E N T C L. A I M S 1. A method of hot-bed field agaric mass treatment c h a- r a c t e r i z. e d i n that the fungus bodies are mechanically purified, disinfected at least for the pe¬ riod of minutes, rinsed with drink water or cleaned by a stream of sterile air, subsequently cut and drie by a sterile medium at temperatures not exceeding 60 C, said mass thereafter being pulverized and formed into a final product suitable for use by a patient, and in addition, sterilized by ionizei ion with use of 8 gamma Co-radiator emitting a dose not exceeding 15 kGy« 2- A method according to claim 1, c h a r a c t e r i z e d i n that said step of disinfection is performed by a solution of chloramine with, concentration thereof of 0,5 to 1,5 %. e A method according to claim 1, c h a r a c t e r i z e d i n that said step- of disinfection is performed by & solution of sorbic acid with concentration thereof of 1 %. « A method according to claim 1, c h a r a c t e r i z e d i n tϊiβt said step- of disinfection is performed by a solution of ethenol with concentration thereof of 50 to 90 %. 5o A method according to claim 1, c h a r a c t e r i z e d i n that said atep of disinfection is performed by a solution of perβcetie ecid with concentration thereof of 0,05 to 0,2 • rSo A method according to claim 1, c h e r a c t e r i z e d i n that said disinfection is performed by the deriva¬ tives of para-hydroxybenzoic acid. 7. A method sccording to claim 1, c h a r a c t e r i z e d i n that said disinfection step is performed by benzoic acid and by the soluble salts thereof with concentration 1' of 1 to 5 % _ 8. A method sccording to claim l. c h a r a c t e r i z e d i n that said disinfection step is performed by ethylen oxide . 9. A method according to claim 1, c h a r a c t e r i z e d i n that said disinfection step, is performed by glutar- aldehyde.

Inventors: RADOSTA JOSEF, RADOSTOVA HANA, RADOSTA, JOSEF, RADOSTOVA, HANA
Applicants: FELIS CO LTD, RADOSTA JOSEF, RADOSTOVA HANA, FELIS CO., LTD.
Application year: 1993
Main classification: A61K
IPC codes: A23L3, A61K36, A61L2