N-ALKYLPIPERIDINYL-4-METHYL CARBOXYLIC ESTERS/AMIDES OF CONDENSED RING SYSTEMS AS 5-HT4 RECEPTOR ANTAGONISTS

Abstract: Compounds of formula (I), wherein formula (I) consists of formulae (I-1) to (I-5), and pharmaceutically acceptable salts thereof, and the use of compound of formula (I) or a pharmaceutically acceptable salt thereof and their use as pharmaceuticals in the treatment of gastrointestinal disorders, cardiovascular disorders and CNS disorders.

Description: N-ALKYLPIPERIDINYL-4-METHYL CARBOXYLIC ESTERS/AMIDES OF CONDENSED RING SUSTEMS AS 5-HT4 RECEPTOR ANTAGONISTS This invention relates to novel compounds having pharmacological activity, to a process for their preparation and to their use as pharmaceuticals. 5 European Journal of Pharmacology 146 (1988), 187-188, and Naunyn-Schmiedeberg's Arch. Pharmacol. (1989) 340:403-410, describe a non classical 5-hydroxytryptamine receptor, now designated the 5-HT4 receptor, and that ICS 205-930, which is also a 5-HT3 receptor antagonist, acts as an antagonist at this receptor. WO 91/16045 (SmithKline and French Laboratories Limited) describes the 10 use of cardiac 5-HT4 receptor antagonists in the treaiment of atrial arrhythmias and stroke. EP-A-501322 (Glaxo Group Limited) describes indole derivatives having 5-HT4 antagonist activity. WO 93/02677, WO 93/03725, WO 93/05038, WO 93/05040 and 15 WO 93/18036 (SmithKline Beecham pic) describe compounds having 5-HT4 receptor antagonist activity. It has now been discovered that certain novel compounds also have 5-HT4 receptor antagonist properties. When used herein, 'treatment' includes prophylaxis as appropriate. 20 Accordingly, the present invention provides compounds of formula (I), wherein formula (I) consists of formulae (I-l) to (1-5), and pharmaceutically acceptable salts thereof, and the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof: (I-l) wherein X and the carbon atoms to which it is attached represents phenyl, cyclohexyl or cyclohexenyl wherein X is -(CH2)4-, and wherein X is optionally substituted 30 by R3 and R 4 ; R\, R2, R3 and R4 are independently hydrogen, halo, C 1.5 al yl, or C \. alkoxy; wherein X is either -Xι-(CH 2 ) X -X2- in which Xι-(CH 2 ) X -X 2 an the aromatic carbon atoms to which they are attached form a 5-7 membered ring wherein one of X j and X2 is O, S or CH2 and the other is CH2 and x is 1, 2 or 3; or X is -X 3 -CH 2 -CH=CH-, -X _(CH 2 )2-CO or -X -(CH 2 )2-CH(OR x )- wherein X 2 is O or S and R x is hydrogen or C 1.5 alkyl; Rj is hydrogen, halo, C\. alkyl, Cμg alkoxy or a ino; R2 and R3 are hydrogen, halo, Cj.g alkyl or C g alkoxy; R4 is hydrogen or C 1.5 alkyl and e is 1 or 2; (1-3) wherein Rj and R 2 are hydrogen, halo, C g alkyl or C\.β alkoxy; or Rj and R 2 are together either Xι-(CH2) X -X2 in which Xι~(CH ) x -X and the aromatic carbon atoms to which they are attached form a 5-7 membered ring wherein one of Xj and X2 is O, S or CH 2 and the other is CH 2 and x is 1, 2 or 3; or Ri and R 2 are together X 3 -CH 2 -CH=CH-, X3-(CH 2 )2-CO or X3-(CH 2 ) 2 -CH(OR x ) wherein X 2 is O or S and R x is hydrogen or Cj.6 alkyU and in which an Rj/R 2 ring may be optionally substituted by one or two C g alkyl groups; R3 is hydrogen, halo, C\. alkyl, Cj.^ alkoxy or amino: R4 is hydrogen, halo, Cj.g alkyl or Cj.fi alkoxy and e is 1 or 2; (1-4) wherein - ~ - represents a single or double bond; R\ is hydrogen, amino, halo, C \. alkyl, hydroxy or C j.g alkoxy; R is hydrogen, halo, C\. alkyl, C\. alkoxy, niu-o, amino or C[. alkylthio; R3 is hydrogen, halo, C 1.5 alkyl, Cj.g alkoxy or amino; R4 is hydrogen or C\. alkyl; (1-5) wherein X is O or S; R is hydrogen, amino, halo, C \. alkyl, hydroxy or C g alkoxy; R 2 is hydrogen, halo, C g alkyl, C\. alkoxy, nitro, amino or Cj_6 alkylthio; R3 is hydrogen, halo, C\, alkyl, C\. alkoxy or amino; R4 is hydrogen or C\ .5 alkyl; In formulae (I-l) to (1-5) inclusive: Y is O or NH; Z is of sub-formula (a), (b) or (c): (a) (b) (c) wherein n 1 is 0, 1, 2, 3 or 4; n 2 is 0, 1, 2, 3 or 4; n 3 is 2, 3, 4 or 5; q is 0, 1, 2 or 3; p is 0, 1 or 2; m is 0, 1 or 2; R5 is hydrogen, Cj.^? alkyl, aralkyl or R5 is (CH 2 ) z -Rιo wherein z is 2 or 3 and Rio is selected from cyano, hydroxyl, Cj.g alkoxy, phenoxy, C(O)Cι_6 alkyl, COC 6 H 5 , -CONRi !R 12 , NR\ ιCOR 12 , Sθ2NRuRi2 θr NRuSθ2Ri2 wherein Rj j and R γ are hydrogen or C g alkyl; and R& R- and Rg are independently hydrogen or Cj.g alkyl; and R9 is hydrogen or C io alkyl; or a compound of formula (I) wherein the CO-Y linkage is replaced by a heterocyclic bioisostere; in the manufacture of a medicament having 5-HT4 receptor antagonist activity. Examples of alkyl or alkyl containing groups include C\, C2, C3, C4, C5, Cg, Cη, Cg, C9, C10, Cn or Cι 2 branched, straight chained or cyclic alkyl, as appropriate. C1.4 alkyl groups include methyl, ethyl, 77- and /sø-propyl, n-, iso-, sec¬ ond t -butyl. Cyclic alkyl includes cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl. Aryl includes phenyl and naphthyl optionally substituted by one or more substituents selected from halo, C \. alkyl and C \ .5 alkoxy. Halo includes fluoro, chloro, bromo and iodo. In formula (1-2): X is often O and A represents a single bond. Rj is preferably methoxy. R 2 is preferably hydrogen or halo. R3 is preferably hydrogen or halo. R4 is often hydrogen. In formula (1-4): R\ is preferably hydrogen or amino. R 2 is preferably hydrogen or halo. R3 is preferably hydrogen or halo. R4 is often hydrogen. In formula (1-5): R\ is preferably hydrogen or amino. R2 is preferably hydrogen or halo. R3 is preferably hydrogen or halo. R4 is often hydrogen. A suitable bioisostere for the amide or ester linkage containing Y in formula (I), is of formula (d): (d) wherein the dotted circle represents one or two double bonds in any position in the 5-membered ring; H, J and I independently represent oxygen, sulphur, nitrogen or carbon, provided that at least one of H, J and I is other than carbon; U represents nitrogen or carbon. Suitable examples of (d) are as described for X, Y and Z in EP-A-328200 (Merck Sharp & Dohme Ltd.), such as an oxadiazole moiety. Y is preferably O or NH. When Z is of sub-formula (a), n^ is preferably 2, 3 or 4 when the azacycle is attached at the nitrogen atom and n* is preferably 1 when the azacycle is attached at a carbon atom, such as the 4-position when q is 2. When Z is of sub-formula (b), n- is preferably such that the number of carbon atoms between the ester or amide linkage is from 2 to 4 carbon atoms. Suitable values for p and m include p = m = 1; p = 0, m = 1, p = 1, m = 2, p = 2, m = 1. When Z is of sub-formula (c), n J is preferably 2, 3 or 4. Rg and R9 are preferably both alkyl, especially one of Rg and R9 is C4 or larger alkyl. Specific values of Z of particular interest arc as follows: (ϋi) ,NMe Bu (iv) The invention also provides novel compounds within formula (I) with side chains (i), (ii), (iii), (iv), (v), (vi) or (vii). In a further aspect, the piperidine ring in (i), (ii) or (iii) may be replaced by pyrroli inyl or azctidinyl, and or the N-substituent in (i) or (ii) may be replaced by C3 or larger alkyl or optionally substituted benzyl. In an alternative aspect, the N-substituent in formula (i) or (ii) may be replaced by (CH 2 ) n R^ as defined in formula (I) and in relation to the specific examples of EP-A-501322. - 1 - The pharmaceutically acceptable salts of the compounds of the formula (I) include acid addition salts with conventional acids such as hydrochloric, hydrobromic, boric, phosphoric, sulphuric acids and pharmaceutically acceptable organic acids such as acetic, tartaric, maleic, citric, succinic, benzoic, ascorbic, methanesulphonic, α-keto glutaric, α-glyccrophosphoric, and glucose- 1 -phosphoric acids. Examples of pharmaceutically acceptable salts include quaternary derivatives of the compounds of formula (I) such as the compounds quaternised by compounds R x -T wherein R x is C\.(, alkyl, phenyl-Ci.g alkyl or C5.7 cycloalkyl, and T is a radical corresponding to an anion of an acid. Suitable examples of R x include methyl, ethyl and n- and wø-propyl; and benzyl and phenethyl. Suitable examples of T include halide such as chloride, bromide and iodide. Examples of pharmaceutically acceptable salts also include internal salts such as N-oxides. The compounds of the formula (I), their pharmaceutically acceptable salts, (including quaternary derivatives and N-oxides) may also form pharmaceutically acceptable solvates, such as hydrates, which are included wherever a compound of formula (I) or a salt thereof is herein referred to. The compounds of formula (I) wherein CO-Y is an ester or amide linkage are prepared by conventional coupling of the Z moiety with the appropriate acid. Suitable methods are as described in GB 2125398 A (Sandoz Limited), GB 1593146A, EP-A-36269, EP-A-289170 and WO 92/05174 (Beecham Group p.l.c). When CO-Y is replaced by a heterocyclic bioisostere, suitable methods are described in EP-A-328200 (Merck Sharp & Dohme Limited). Reference is also made to EP- A-501322 (Glaxo Group Limited) . The invention also comprises a process for preparing the novel compounds of formula (I) which comprises reacting an appropriate acid derivative with an appropriate alcohol or amine. A process comprises reacting an acid derivative wherein the aromatic substituents are as required in the end compound of formula (I), or substituents convertible thereto, with an alcohol or amine containing Z or a group convertible thereto, and thereafter if necessary, converting the benzoic acid substituents and or Z, and optionally forming a pharmaceutically acceptable salt. Suitable examples of conversions in the aromatic substituents include chlorination of hydrogen to chloro, reduction of nitro to amino, dehydrohalogenation such as debromination. Any elaboration is, however, usually carried out prior to ester or amide coupling. Suitable examples of conversions in the Z containing moiety include conventional modifications of the N-substituent by substitution and or deprotection or, in the case of a 2-, 3- or 4- substituted piperidinyl desired end compound, reduction of an appropriate pyridyl derivative. The compounds of the present invention are 5-HT4 receptor antagonists and it is thus believed may generally be used in the treatment or prophylaxis of gastrointestinal disorders, cardiovascular disorders and CNS disorders. They are of potential interest in the treatment of irritable bowel syndrome (IBS), in particular the diarrhoea aspects of IBS, i.e., these compounds block the ability of 5-HT to stimulate gut motility via activation of enteric neurones. In animal models of IBS, this can be conveniently measured as a reduction of the rate of defaecation. They are also of potential use in the treatment of urinary incontinence which is often associated with IBS. They may also be of potential use in other gastrointestinal disorders, such as those associated with upper gut motility, and as antiemetics. In particular, they are of potential use in the treatment of the nausea and gastric symptoms of gastro- oesophageal reflux disease and dyspepsia. Antiemetic activity is determined in known animal models of cytotoxic-agent/radiation induced emesis. Specific cardiac 5-HT4 receptor antagonists which prevent atrial fibrillation and otiier atrial arrhythmias associated with 5-HT, would also be expected to reduce occurrence of stroke (see AJ. Kaumann 1990, Naumyn-Schmiedeberg's Arch. Pharmacol. 342, 619-622, for appropriate animal test method). Anxiolytic activity is likely to be effected via the hippocampus (Dumuis et al 1988, Mol Pharmacol., 34, 880-887). Activity can be demonstrated in standard animal models, the social interaction test and the X-maze test. Migraine sufferers often undergo situations of anxiety and emotional stress that precede the appearance of headache (Sachs, 1985, Migraine, Pan Books, London). It has also been observed that during and within 48 hours of a migraine attack, cyclic AMP levels are considerably increased in the cerebrospinal fluid (Welch et al, 1976, Headache 16, 160-167). It is believed that a migraine, including the prodomal phase and the associated increased levels of cyclic AMP are related to stimulation of 5-HT4 receptors, and hence that administration of a 5-HT4 antagonist is of potential benefit in relieving a migraine attack. Other CNS disorders of interest include schizophrenia, Parkinson's disease and Huntingdon's chorea. The invention also provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier. Such compositions are prepared by admixture and are usually adapted for enteral such as oral, nasal or rectal, or parenteral administration, and as such may be in the form of tablets, capsules, oral liquid preparations, powders, granules, lozenges, reconstitu table powders, nasal sprays, suppositories, injectable and infusable solutions or suspensions. Orally administrable compositions are preferred, since they are more convenient for general use. Tablets and capsules for oral administration are usually presented in a unit dose, and contain conventional excipients such as binding agents, fillers, diluents, tabletting agents, lubricants, disintegranls, colourants, flavourings, and wetting agents. The tablets may be coated according to well known methods in the art, for example with an enteric coating. Suitable fillers for use include cellulose, mannitol, lactose and other similar agents. Suitable disintegrants include starch, polyvinylpolypyrrolidone and starch derivatives such as sodium starch glycollate. Suitable lubricants include, for example, magnesium stearate. Suitable pharmaceutically acceptable wetting agents include sodium lauryl sulphate. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate. or acacia; non-aqueous vehicles (which may include edible oils), for example, almond oil, fractionated coconut oil, oily esters such as esters of glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl / hydroxybenzoate or sorbic acid, and if desired conventional flavouring or colouring agents. Oral liquid preparations are usually in the form of aqueous or oily suspensions, solutions, emulsions, syrups, or elixirs or are presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, emulsifying agents, non-aqueous vehicles (which may include edible oils), preservatives, and flavouring or colouring agents. The oral compositions may be prepared by conventional methods of blending, filling or tabletting. Repeated blending operations may be used to distribute the active agent throughout those compositions employing large quantities of fillers. Such operations are, of course, conventional in the art. For parenteral administration, fluid unit dose forms are prepared containing a compound of the present invention and a sterile vehicle. The compound, depending on the vehicle and the concentration, can be either suspended or dissolved. Parenteral solutions are normally prepared by dissolving the compound in a vehicle and filter sterilising before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, preservatives and buffering agents are also dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and sterilised by exposure of ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound of the invention. The invention further provides a method of treatment or prophylaxis of irritable bowel syndrome, dyspepsia, atrial arrhythmias and stroke, anxiety and/or migraine in mammals, such as humans, which comprises the administration of an effective amount of a compound of the formula (I) or a pharmaceutically acceptable salt thereof. An amount effective to treat the disorders hereinbefore described depends on the relative efficacies of the compounds of the invention, the nature and severity of the disorder being treated and the weight of the mammal. However, a unit dose for a 70kg adult will normally contain 0.05 to lOOOmg for example 0.5 to 500mg, of the compound of the invention. Unit doses may be administered once or more than once a day, for example, 2, 3 or 4 times a day. more usually 1 to 3 times a day, that is in the range of approximately 0.0001 to 50mg/kg/day, more usually 0.0002 to 25 mg/kg/day. No adverse toxicological effects are indicated within the aforementioned dosage ranges. The invention also provides a compound of formula (I) or a pharmaceutically acceptable salt thereof for use as an active therapeutic substance, in particular for use in the treatment of irritable bowel syndrome, gastro-oesophageal reflux disease, dyspepsia, atrial arrhythmias and stroke, anxiety and/or migraine. The following Examples illustrates the preparation of compounds of formula (I), and the following Descriptions relate to the preparation of intermediates. The compounds of formula (I-l) and intermediates are prepared in Examples and Descriptions 1-1, 2-1 etc, the compounds of formula (1-2) are prepared in Examples and Descriptions 1-2, 2-2 etc and similarly for the compounds of formulae (1-3) to (I- 5). It will be appreciated that any compound prepared wherein Y is O may be provided as the corresponding compound wherein Y is NH. Example (1-1) [R 1 ? R 2 , R3, R4, H, X is cyclohexyl, Y=O, Z=(i)] N-Carboxy-[l-butyl-4-piperidinylmethyl]-l,2,3,4,4a,9a-hexahydrocarbazoIe l-Butyl-4-piperidinylmethanol (0.500g 2.12mmol) was dissolved in dry THF (3ml) and treated with methyllithium (1.5M solution in diethyl ether) 2.16ml, 2.33mmol) with stirring under nitrogen. After 10 minutes, N-chlorocarbonyl- l,2,3,4,4a,9a-hexahydrocarbazole (0.630g, 2.68mmol) (Ref: WO 89/09217) in dry THF (5ml) was added dropwise with stirring. After 48 hours, the reaction mixture was filtered and the filtrate evaporated under reduced pressure. The residue was purified by silica-gel chromatography, eluting with 2% MeOH/CHCl3- 3%MeOH CHCl3 to give the title compound.as a clear, colourless oil, which was converted to the oxalate salt, mp 187- 189°C. !H NMR (250MHz, CDCI3) (free base) 5: 7.90-7.60 (s br, 1H) 7.25-7.10 (m, 2H), 7.00 (t. 1H), 4.45-4.30 (m br, 1H), 4.10 (d, 2H), 3.45 (t, 1H), 3.00 (d, 2H), 2.45-2.20 (m, 3H), 2.10-1.95 (m, 3H),1.85-1.70 (m, 4H), 1.65-1.40 (m, 6H), 1.40-1.10 (m, 6H), 0.90 (t, 3H). Examples 2-1 and 3-1 [R , R 2 , R3, R4, = H, X is cyclohexenyl/phenyl, X = O, Z = (i)] N-Carboxy-[l-butyI-4-piperidinylmethyl]-l,2,3,4-tetrahydrocarbazoIe N-Carboxy-[l-butyI-4-piperidinylmethyI]-carbazole N-Carboxy-[l-butylpiperidin-4-ylmethyl]-l, 2,3,4 ,4a,9a-hexahydrocarbazole (0.530g, 1.32mmol) was dissolved in chloroform (25 ml) and treated with 2,3- dichloro-5,6-dicyano-l, 4-benzoquinone (0.329g, 1.58mmol) with stirring. The mixture was heated to reflux for 12 hours, cooled, and more 2,3-dichloro-5,6-dicyano -1,4- benzoquinone (0.329g 1.58mmol) added with stirring. The mixture was heated to" reflux for 12 hours, cooled and evaporated under reduced pressure. The residue was purified by Silica-gel chromatography, eluting with 2%MeOH/CHCl3 to give the title compounds as 3:1 mixture. The mixture was separated by preparative HPLC using a Waters Micro Bondapak C18 (300mm x 7.8mm) column and eluting with 75:25 MeCN: 0.1M Ammonium Acetate (pH 5.0). (2-1) *H NMR (400MHz, CDCl3) δ: 8.10 (d, 1H), 7.40 (d, 1H), 7.28-7.18 (m, 2H), 4.28 (d, 2H), 3.03-2.95 (m,4H),2.68-2.62 (m, 2H), 2.35 (t, 2H), 2.00-1.80 ( , 5H), 1.53-1.38 (m, 4H), 1.35-1.25 (m, 3H), 0.92 (t, 3H). (3-1) !H NMR (400MHz, CDC1 3 ) δ: 8.28 (d, 2H), 8.00 (d, 2H), 7.50 (t, 2H), 7.38 (t, 2H), 4.48 (d, 2H), 3.05-2.95 (m, 4H), 2.65-2.60 (m, 2H), 2.35 (t, 2H), 2.00-1.75 (m,5H), 1.40-1.30 (m, 3H), 0.95 (t, 3H). Example 1-2 [R l f R , R3, R4, = H, X = O-(CH 2 )3-, Y = 0, Z = (i)] l-Butylpiperidin-4-y]methyl-3,4-dihydro-2H-naphtho[2,l-b]pyran-10- carboxylate 3,4-Dihydro-2H-naphtho[2,l-b]ρyran-10-carboxylic acid (0.510g, 2.24 mmol) was dissolved in CH 2 C1 2 (20 ml) and treated with oxalyl chloride (0.292 ml, 3.35 mmol), followed by a drop of dry DMF with stirring under N 2 . After 3h, the reaction mixture was evaporated under reduced pressure and dried in vacito to give the crude acid chloride. Meanwhile, a solution of l-butylpiperidin-4-yl methanol (0.363g, 2.12 mmol) in dry THF (10 ml) was treated with 1.5M methyllithium (1.12 ml, 2.12 mmol). After 0.25h to this solution was added the crude acid chloride in dry THF (10 ml) with stirring under N 2 . After 40h, the reaction mixture was evaporated under reduced pressure and the residue partitioned between EtOAc and water. The aqueous layer was then extracted with EtOAc (lx) and the combined organic layers were dried (Na 2 SO4) and evaporated under reduced pressure to give a yellow oil which was purified by silica gel chromatography (EtOAc as eluant) to give the title compound as a pale yellow oil (0.180g, 21%) which was converted to its hydrochloride salt m.p. (HCl salt) 137-139°C. 1H NMR (CDCI3) 250 MHz (free base) δ: 8.14(lH,s), 7.80(2H,m), 7.58(1H, t, J=7Hz), 7.40(1H, t, J=7Hz), 4.30(2H, t, J=6Hz), 4.20(2H, d, J=6Hz), 3.08(2H, t, J=6Hz), 2.98(2H, d. J=12Hz), 2.35(2H, t, J=6Hz), 2.20(2H,m), 2.00-1.70(6H, m), 1.60-1.25(5H,m), 0.92(3H, t, J=6Hz) Example 2-2 [R R 2 , R3, R 4 , = H, X = -O-CH 2 -CH=CH-, Y = O, Z = (i)], l-ButyIpiperidin-4-ylmethyl-2H-naphtho[2,l-b]pyran-10-carboxylate This was prepared from 2H-naphtho[2,l-b]pyran-10-carboxylic acid using the method outlined in Example 1. l U NMR (CDCI3) 200 MHz δ: 8.20(lH,s), 7.90(lH,d,J=8Hz), 7.80(lH,d, J=8Hz), 7.55(1H, t, J=8Hz), 7.40(lH,t,J=8Hz), 7.10(1H, d, J=12Hz), 6.00 (1H, m), 4.92(2H, m), 4.20(2H, d, J=6Hz), 3.00(2H, d, J=12 Hz), 2.35(2H, t, J=6Hz), 2.05-l,75(5H,m), 1.60-1.32(6H, m), 0.95(3H, t, J=6Hz). Description 1-2 (intermediate for Example 1-2) a) Methyl-3-propargyloxy-2-naphthoate Methyl-3-hydroxy-2-naphthoate (2.63g, 0.013 mol) was dissolved in dry THF (80 ml) and treated with sodium hydride (80%) ((0.398g, 0.013 mol), with stirring under N 2- After 0.5h, propargyl bromide (80% in toluene) (2.57 ml 0.017 mol) was added and the mixture heated to reflux. After 20h, the reaction mixture was allowed to cool and then evaporated under reduced pressure. The residue was then evaporated under reduced pressure. The residue was then partitioned between EtOAc and water. The aqueous layer was then extracted with EtOAc (lx), and the combined organic layers were dried (Na 2 SO4) and evaporated to given an orange oil. Crystallisation of the oil from petrol (60/80) diethyl ether provided the title compound as a pale yellow solid (l.Ogg, 35%). l H NMR (CDC1 3 ) 250 MHz δ: 8.34(1H, s), 7.80(lH,dd, J=16 and 8 Hz), 7.53(1H, t, J=8Hz), 7.40(1H, t,J=8 Hz), 4.90(2H, d,J=lHz), 3.95(3H, s), 2.57(1H, t, J=lHz) b) Methyl-2H-naphtho[2,l-b]pyran-lϋ-carboxylate Methyl-3-propargyloxy-2-naphthoate (Dl) (l.OOg, 4.17 mmol) was dissolved in l,2,dichlorobenzene (50 ml) and heated to reflux under N 2 with stirring. After 64h, the reaction mixture was allowed to cool and was then evaporated under reduced pressure. The brown oily residue was then purified by silica-gel chromatography (Pentane: EtOAc 4:1 as eluant) to give the title compound to an orange oil (0.801g, 80%). Η NMR (CDCI3) 250 MHz δ: 8.20(lH,s), 7.92(1H, d, J=8 Hz), 7.80(1 H, d, J=8Hz), 7.54 (1H, t, J=8Hz), 7.40(lH,t,J=8Hz), 7.12(1H, d,J=8 Hz), 6.00(lH,m), 4.94(2H,m), 3.94(3H,s) c) Methyl-3,4-dihydro-2H-naphtho[2,l-b]pyran-10-carboxyIate Methyl-2H-naphtho[2,l-b]pyran-10-carboxylate (0.590g, 2.46 mmol) was dissolved in ethyl acetate (30 ml) and 5% PdC (0.2g) was added under N 2 . The mixture was then hydrogenated at atmospheric pressure. When theoretical H 2 uptake had been achieved, the reaction mixture was flushed with N 2 and then filtered through a pad of celite. The filtrate was then evaporated under reduced pressure and dried in vacuo to give the title compound as a pale yellow oil (0.154g, 91%). 1 H NMR (CDCI 3 ) 200 MHz δ: 8.20 (1H, s), 7.82 (1H, d, J=8 Hz), 7.58(1H, t, J=8Hz), 7.40(1H, t, J=8 Hz), 4.37(2H, t, J=6Hz), 3.98(3H, s), 3.12(2H, t, J=6Hz), 2.25(2H,m). d) 3,4-Dihydro-2H-naphtho[2.1-b]pyran-10-carboxyIic acid Methyl-2H-3,4,dihydronaphtho[2.1-b]pyran-10-carboxylate (0.530g, 2.19 mmol) was dissolved in eti anol (15 ml) and 10% NaOH (15 ml) was added. The mixture was then heated to reflux with stirring. After 6h, the reaction mixture was allowed to cool and the ethanol present was removed by evaporation under reduced pressure. The aqueous residue was then acidified to pHl using cone. hydrochloric acid and the resultant oily suspension was extracted with CHCI3 (3x). The combined organic layers were then dried (Na 2 SO4) and evaporated under reduced pressure to give a pale yellow oil that crystallised on standing (0.499g, 100%). 1H NMR (CDCI3) 200 MHz δ: 12.0-10.5 br (lH,s), 8.60(lH,s), 7.87(1H, d, J=8 Hz), 7.78(1H, d, J=8 Hz), 7.58(1H, t, J=8 Hz), 7.38(1H, t, J=8 Hz) 4.44(2H, t, J=6Hz), 3.12(2H, t, J=6Hz), 2.25(2H,m). Description 2-2 (intermediate for Example 2-2) 2H-Naphtho[2,l-b]pyran-10-carboxyIic acid This was prepared from methyl-2H-naphtho[2,l-b]pyran-10-carboxylate using the method oudined in Description l-2d). *H NMR (CDCI3) 200 MHz δ: 8.60(lH,s), 7.90(2H,m), 7.60(lH,t,J=8Hz), 7.40(1H, t, J=8 Hz), 7.15 (IH, d, J=12 Hz), 6.08 (IH, m), 5.05(2H,m) Example 1-3 [R R , R3, R , = H, Y = O, Z = (i)] [l-Butylpiperidin-4-yImethyInaphthaIene-l-carboxylate To a slurry of 1-naphthoic acid (500mg) in dichloromethane (30ml) was added oxalyl chloride (510ml) and N,N' dimethylformamide(2 drops). The resulting mixture was stirred at room temperature for 1 hour. The solvent was concentrated in vacuo to afford crude acid chloride. Methyllithium (2.14 ml ofl.5M soln. in diethyl ether) was added dropwise to a solution of l-butyl-4-hydroxymethylpiperidine (500mg) in dry THF (10ml) at 0°C. Stirring was continued at 0°C for 10 minutes. A solution of crude acid chloride in THF (10ml) was added to the reaction mixture and stirring continued at room temperature overnight. Water (2ml) was added and the solvent concentrated under reduced pressure. The residue was partitioned between chloroform and water. The organic phase was dried (Na 2 SO4), filtered and concentrated in vacuo. The residue was chro atographed on silica using chloroform and ethanol as eluant to afford pure ester (502mg). Treatment with ethereal HCl gave the title compound as a solid. l H NMR 250MHz (CDCI3) Free base δ: 8.92(d,lH), 8.19(d,lH), 8.03(d,lH), 7.89(d,lH), 7.44-7.65(m,3H), 4.28(d,2H), 2.99(d,2H), 2.32(t,2H), 1.71-2.02(m,5H), 1.43-1.58(m,4H), 1.23-1.41(m,2H), 0.9(t,3H). Example 1-4 [™ is single bond, Rj, R 2 , R3, R4 = H, Y = O, Z = (i)] 7-(l-Butyl-4-piperidinylmethyl)-lH-2,3-dihydroindolecarboxylate Indoline-7-carboxylic acid (Ger.Offen.2,117,116) is coupled with lithium-(l-butyl-4- piperidinyl)methoxide via the imidazolide The following compounds are prepared similarly, using the appropriate acid : Example 2-4 [^ is single bond, R 1 = H, R 2 =C1, R3, R4 = H,Y = O, Z = (i)] [7-(l-Butyl-4-piperidinylmethyl)-5-chloro-lH-2,3-dihydroindolecarboxylate Example 3-4 [^ is double bond, R\, R2, R3, R4 = H, Y = O, Z = (i)] 7-(l-Butyl-4-piperidinylmethyl)-lH-indoIecarboxylate Example 4-4 [^ is double bond, Ri = H, R 2 =C1, R3, R4 = H,Y = O, Z = (i)] 7-(l-Butyl-4-piperidinylmethyl)-5-chloro-lH-indolecarboxylate Description 1-5 N-(l-Butyl-4-piperidinyl)methyIamine A stirred solution of isonipecotamide (70g, 0.55 mole) and 1-bromobutane (58.8 ml, 0.55 mole) in ethanol (700 ml) was treated with anhydrous potassium carbonate (152g, 1.10 mole) and heated under reflux for 3h. The mixture was allowed to cool, then filtered and the filtrate concentrated under vacuum. The residual oil was dissolved in chloroform (400 ml) and washed with water (1 x 300 ml), then dried (Na2SO4) and concentrated under vacuum to leave a yellow oil (77.5g). This oil was mixed thoroughly with phosphorus pentoxide (75g) and the mixture heated at 160-180°C under nitrogen for 2.5h with gentle stirring. The reaction mixture was allowed to cool, then treated with water (500 ml). When the solid mass had dissolved, the solution was basified by addition of solid K 2 CO3 and extracted with ethyl acetate (2x400 ml). The combined extracts were dried (Na 2 SO4) and concentrated in vacuo to leave a brown oil (78g). This was dissolved in dry ether (400 ml) and added dropwise over 30 minutes to a stirred suspension of lithium aluminium hydride (25g, 0.66 mole) in ether (200ml) at 0°C under nitrogen. When addition was complete, the mixture was allowed 10 warm upto room temperature and stir for 18h. It was re-cooled to 0°C and treated cautiously with water (25ml), 10% NaOH solution (25 ml) and water again (75ml). The mixture was filtered through kieselguhr and the filtrate concentrated in vacuo to leave a brown oil, which was distilled under vacuum to afford the title compound as a colourless oil (66g, 71%) bp 96-99°C at 3 mm Hg. l H NMR (CDC1 3 ) δ: 2.90-3.02(m,2H), 2.58(d,2H), 2.25-2.38(m,2H), 1.65-2.00(m,4H), 1.08- 1.58(m,9H), 0.92(t,3H). Example 1-5 [Rγ = H, R 2 = Cl, R3, R , = H, Y = O, Z = (i)] N-[(l-Butyl-4-piperidinyI)methyl] 5-chloro-2-methyIbenzoxazole-7-carboxamide 5-Chloro-2-methylbenzoxazole-7-carboxylic acid (Ger. Offen. 2,225,544) is converted to its acid chloride by treatment with oxalyl chloride. The acid chloride is treated with N-(l-butyl-4-piperidinyl)methylamine in the presence of triethylamine to afford the title compound. 5-HT4 RECEPTOR ANTAGONIST ACTIVITY 1) Guinea pig colon Male guinea-pigs, weighing 250-400g are used. Longitudinal muscle- myenteric plexus preparations, approximately 3cm long, are obtained from the distal colon region. These are suspended under a 0.5g load in isolated tissue baths containing Krebs solution bubbled with 5% CO2 in O2 and maintained at 37°C. In all experiments, the Krebs solution also contains methiothepin 10 ' ^M and granisetron 10 _6 M to block effects at 5-HTι, 5-HT 2 and 5-HT3 receptors. After construction of a simple concentration-response curve with 5-HT, using 30s contact times and a 15min dosing cycle, a concentration of 5-HT is selected so as to obtain a contraction of the muscle approximately 40-70% maximum(10 " ^M approx). The tissue is then alternately dosed every 15min with this concentration of 5-HT and then with an approximately equi-effective concentration of the nicotine receptor stimulant, dimethylphenylpiperazinium (DMPP). After obtaining consistent responses to both 5-HT and DMPP, increasing concentrations of a putative 5-HT4 receptor antagonist are then added to the bathing solution. The effects of this compound are then determined as a percentage reduction of the contractions evoked by 5-HT or by DMPP. From this data, PIC50 values are determined, being defined as the -log concentration of antagonist which reduces the contraction by 50%. A compound which reduces the response to 5-HT but not to DMPP is believed to act as a 5-HT4 receptor antagonist. The compounds generally had a PIC50 of at least 7.

Claims: Claims 1. Compounds of formula (I), wherein formula (I) consists of formulae (I-l) to (1-5), and pharmaceutically acceptable salts thereof, and the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof: (I-l) wherein X and the carbon atoms to which it is attached represents phenyl, cyclohexyl or cyclohexenyl wherein X is -(CH 2 )4-, and wherein X is optionally substituted by R3 and R4; R , R 2 , R3 and R4 are independently hydrogen, halo, C . alkyl, or C g alkoxy; (1-2) wherein X is either -Xι-(CH 2 ) X -X 2 - in which X ι-(CH 2 ) x -X2 and the aromatic carbon atoms to which they are attached form a 5-7 membered ring wherein one of X and X 2 is O, S or CH 2 and the other is CH 2 and x is 1, 2 or 3; or X is -X 3 -CH 2 -CH=CH-, -X 3 .(CH 2 ) 2 -CO or -X 3 -(CH 2 )2-CH(OR x )- wherein X 2 is O or S and R x is hydrogen or C j.g alkyl; R is hydrogen, halo, C g alkyl, C\.β alkoxy or amino; R 2 and R3 are hydrogen, halo, C g alkyl or C 1. alkoxy; R4 is hydrogen or C g alkyl and e is 1 or 2; (1-3) wherein R and R 2 are hydrogen, halo, C\.β alkyl or Cj.g alkoxy; or Rj and R 2 are together either Xι-(CH 2 ) X -X 2 i which Xι-(CH2) -X2 and the aromatic carbon atoms to which they are attached form a 5-7 membered ring wherein one of X and X 2 is O, S or CH2 and the other is CH2 and x is 1, 2 or 3; or Ri and R2 are together X3-CH -CH=CH-, X3-(CH 2 ) -CO or X3-(CH 2 ) -CH(OR x ) wherein X 2 is O or S and R x is hydrogen or C1-6 alkyl; and in which an Rι/R 2 ring may be optionally substituted by one or two C g alkyl groups; R3 is hydrogen, halo, C g alkyl, C g alkoxy or amino; R4 is hydrogen, halo, C\..$ alkyl or Cμg alkoxy and e is 1 or 2; (1-4) wherein '- represents a single or double bond; R is hydrogen, amino, halo, C 1. alkyl, hydroxy or C . alkoxy; R 2 is hydrogen, halo, C\. alkyl, C g alkoxy, nitro, amino or C g alkylthio; R3 is hydrogen, halo, C g alkyl, C j.g alkoxy or amino; R4 is hydrogen or C g alkyl; (1-5) wherein X is O or S; Rl is hydrogen, amino, halo, Cj.g alkyl, hydroxy or C\.Q alkoxy; R 2 is hydrogen, halo, C g alkyl, C g alkoxy, nitro, amino or C\. alkylthio; R3 is hydrogen, halo, C\. alkyl, C g alkoxy or amino; R4 is hydrogen or C \.β alkyl; In formulae (I-l) to (1-5) inclusive: Y is O or NH; Z is of sub-formula (a), (b) or (c): (a) (b) (c) wherein n 1 is 0, 1, 2, 3 or 4; n 2 is 0, 1, 2, 3 or 4; n 3 is 2, 3, 4 or 5; q is 0, 1, 2 or 3; p is 0, 1 or 2; m is 0, 1 or 2; R5 is hydrogen, Cι_ι 2 alkyl, aralkyl or R5 is (CH 2 ) z -Rιo wherein z is 2 or 3 and Rio is selected from cyano, hydroxyl, C g alkoxy, phenoxy, C(O)Cι_ 6 alkyl, COC 6 H 5 , -CONRi χR 12 , NRi ιCORι 2 , SO 2 NR 11 R 12 or NR \ SO R 12 wherein R 1 and R 12 are hydrogen or Cι_6 alkyl; and R , Rη and Rg are independently hydrogen or Cj.g alkyl; and R9 is hydrogen or C alkyl; or a compound of formula (I) wherein the CO-Y linkage is replaced by a heterocyclic bioisostere; in the manufacture of a medicament having 5-HT4 receptor antagonist activity. 2. A compound according to claim 1 wherein: in formula (1-2): X is O and A represents a single bond. Ri is methoxy. R 2 is hydrogen or halo. R3 is hydrogen or halo. R4 is hydrogen. In formula (1-4): R is hydrogen or amino. R 2 is hydrogen or halo. R3 is hydrogen or halo. R4 is hydrogen. In formula (1-5): Ri is hydrogen or amino. R 2 is hydrogen or halo. R3 is hydrogen or halo. R4 is hydrogen. 3. A compound according to claim 1 or 2 wherein the moiety attached to CO-Y-Z is that which is as contained in any of the Examples described herein. 4. A compound according to any one of claims 1 to 3 wherein Z is of sub-formula (a) and (CH 2 ) n l is attached at a carbon atom of the azacycle. 5. A compound according to claim 4 wherein Z is X-substituted 4-piperidylmethyl. 6. A compound according to claim 5 wherein the N-substituent is C2 or greater alkyl, or optionally substituted benzyl. 7. N-Carboxy-[l-butyl-4-piperidinylmethyl]-l,2,3.4,4a,9a-hexahydrocarbazole, N-carboxy-[l-butyl-4-piperidinylmethyl]-l,2,3,4-tetrahydrocarbazole, or N-carboxy-[l-butyl-4-piperidinylmethyl]-carbazole. 8. l-Butylpiperidin-4-ylmethyl-3,4-dihydro-2H-naphtho[2,l-b]pyran-10- carboxylate, or l-butylpiperidin-4-ylmethyl-2H-naphtho[2,l-b]pyran-lϋ-carboxylate. 9. [l-Butylpiperidin-4-ylmethylnaρhthalene-l-carboxylate. 10. 7-(l-Butyl-4-piperidinylmethyl)-lH-2,3-dihydroindolecarboxylate, [7-(l-butyl-4-piperidinylmethyl)-5-chloro-lH-2,3-dihydroindolecarboxylate, 7-(l-butyl-4-piperidinylmethyl)-lH-indolecarboxylate, or 7-(l-butyl-4-piperidinylmethyl)-5-chloro- lH-indolecarboxylate. 11. N-[(l-Butyl-4-piperidinyl)methyl] 5-chloro-2-methylbenzoxazole-7- carboxamide 12. A compound according to any one of claims 7 to 11 in the form of a pharmaceutically acceptable salt. 13. A compound according to any one of claims 7 to 12 but wherein Y is NH. 14. A process for preparing the ester or amide compounds (where Y is O or NH) according to claim 1, which comprises reacting an appropriate acid derivative with an appropriate alcohol or amine. 15. A pharmaceutical composition comprising a compound according to any one of claims 1 to 13, and a pharmaceutically acceptable carrier. 16. A compound according to claim 1 for use as an active therapeutic substance. 17. The use of a compound according to claim 1 in the manufacture of a medicament for use as a 5-HT4 receptor antagonist. 18. The use according to claim 17 for use as a 5-HT4 receptor antagonist in the treatment or prophylaxis of gastrointestinal disorders, cardiovascular disorders and CNS disorders.

Inventors: GASTER LARAMIE MARY, JOINER GRAHAM FRANCIS, MULHOLLAND KEITH RAYMOND, WYMAN PAUL ADRIAN, GASTER, LARAMIE, MARY, JOINER, GRAHAM, FRANCIS, MULHOLLAND, KEITH, RAYMOND, WYMAN, PAUL, ADRIAN
Applicants: GASTER LARAMIE MARY, JOINER GRAHAM FRANCIS, MULHOLLAND KEITH RAYMOND, SMITHKLINE BEECHAM PLC, WYMAN PAUL ADRIAN
Application year: 1993
Main classification: C07D211
IPC codes: A61K31, A61P1, A61P9, A61P25, C07C229, C07C233, C07C235, C07D211, C07D401, C07D405, C07D413