MULTIFUNCTIONAL LIGAND FOR USE AS A DIAGNOSTIC OR THERAPEUTIC PHARMACEUTICAL

Abstract: A compound and method of making a compound for use as a diagnostic or therapeutic pharmaceutical comprises either a phosphorous or germanium core and at least two hydrazine groups forming a ligand for bonding to a metal extending from the phosphorous or germanium core.

Description: RELATION TO OTHER APPLICATION This application is a continuation-in- part of copending application Serial No. 694,142, filed May 1, 1991. MϋLTIFUNCTIONAL LIGAND FOR USE AS A DIAGNOSTIC OR THERAPEUTIC PHARMACEUTICAL TECHNICAL FIELD The present invention relates to pharmaceuticals, and especially radiopharmaceuticals for use as diagnostic and therapeutic agents. More specifically, the present invention relates to a compound and method of synthesizing a compound which is a multidentate ligand that complexes with transition metal radionuclides for use as diagnostic or therapeutic radiopharmaceuticals. BACKGROUND OF THE INVENTION Radiotherapy using "non-sealed sources" by way of radiolabeled pharmaceuticals has been employed for several decades (1-3) . Unfortunately, less than a handful of therapeutic radiopharmaceuticals are currently in routine use, as being approved by the FDA. There has been renewed interest in developing new agents due to the emergence of more sophisticated molecular carriers, such as monoclonal antibodies that are capable of selectively targeting cancerous lesions. In addition, the identification of several different radionuclides (4-7) with different chemical properties that have physical decay properties that are desirable for therapeutic application have further spurred development of new agents. Despite some successes in treatment of specific malignant diseases and increased research and development activities in this area, many problems remain with the use of such treatments. For example, in most cancers, it has been difficult to provide acceptable selectivity in radiation doses delivered to target tissues relative to normal tissues. Successful development of new therapeutic radiopharmaceuticals requires improved localization of these agents in target tissues and/or increasing rates of clearance from non- target tissues. In both of these cases, it is imperative that the therapeutic radionuclide remain firmly associated with the radioactive drug in vivo for extended periods of time. These periods of time can extend from a few hours up to several days, depending on the pharmaco inetics and physical half-life of the radionuclide. No single radionuclide can be appropriate in formulating therapeutic agents since different half-lives and the energy of emitted particles is required for different applications (4-7) thereby making it essential that radiopharmaceuticals with different radionuclides be available. Therapeutic agents have been primarily labeled with beta-particle emitting radionuclides. Most of the promising radionuclides are produced in nuclear reactors, however, some are accelerator produced (4-7) . Several different chelating structures have been employed to maintain the association of these beta emitters with the drug (8-12) . Many of these structures are not sufficiently stable and most, if not all, do not provide appropriate routes or rates of clearance of radioactivity from non-target tissues (13-14) . Accordingly, there is a delivery of high radiation doses to normal tissues and a reduction of the therapeutic ratio. This lowers the amount of radiation dose that can be safely delivered to a target tissue. Development of new radionuclide chelates that link the radioactive metal to the radiopharmaceutical is necessary. Further, new approaches must be taken in order to identify radio-labeling techniques that produce chelates that are highly stable in vivo but have improved clearance characteristics from normal tissues. Bi-functional chelating agents have been used to form stable metal complexes that were designed to minimize in vivo release of the metallic radionuclide from the radiopharmaceutical. For example, diethyltriaminepentaacetic acid (DTPA) forms rather stable chelates with a variety of metals. However, coupling of this ligand to monoclonal antibodies by one of its five carboxyl groups resulted in unacceptable in vivo stability with a variety of radionuclides (15) . Linking of this compound by a side group attached to one of the carbon atoms on an ethylene bridging group provides improved stability in vitro and in vivo. The stability characteristics of these compounds are not ideal resulting in poor clearance of activity from certain non-target organs are poor. Chelating agents based on diamido- dithiol and triamidomonothiol backbones are used for forming small and stable hydrophilic complexes with several beta-emitting transition metals. These were developed by Fritzberg and colleagues (10) for labeled monoclonal antibody products for diagnostic and therapeutic applications. These chelates provide improved clearance characteristics from the liver, however, kidney retention of activity when using Fab or (Fab) 2 fragments of monoclonal antibodies labeled with these radionuclide chelates is higher than desirable. A macrocyclic tetramine-based chelating agent that also has four methylene carboxylate side atoms has been used to form a copper complex that has a high in vitro and in vivo stability when linked to monoclonal antibodies. This chelate was first described for monoclonal antibody bioconjugation by Meares and coworkers (11,12). The chelate is rather large. Clearance of activity from non-target organs has not been shown to be more efficient than the chelates mentioned above. Recently, a mono-hydrazide bifunctional chelating agent has been described that forms somewhat stable 99m Tc complexes (16,17). This particular ligand can be first attached to a protein and then binds to 99m Tc as it is chelated with glucoheptonate in aqueous solutions at or near neutral pH (17) . Other work with mono- hydrazide ligands with other Tc v complexes demonstrates the reaction of the monohydrazides at the axial position with Tc v =0 to form similar mondentate linkages (16) . The in vitro and in vivo stability of these types of chelates has not been adequately described in the literature. Applicant has synthesized a series of multi-dentate ligands derived from a phosphorous or germanium core utilizing hydrazine groups as arms of these ligands to form small but stable and well defined complexes with transition metal radionuclides. Unlike prior art chelates, these chelates show good stability in both aqueous solutions, serum, and other body fluids. SUMMARY OF THE INVENTION In accordance with the present invention, there is provided a compound for use as a diagnostic or therapeutic pharmaceutical, the compound comprising a phosphorous or germanium core and at least two hydrazine groups forming a ligand for bonding to a metal extending from the phosphorous or germanium core. The present invention further provides a method of making the compound for use as a diagnostic therapeutic pharmaceutical, the method including the following reaction Cl / E=P—Cl \ X R 5 R 6 I I Wherein R 2 = H; X is N-N-H, alkyl, (Me, Et, n-or i - propyl, n-, i-or t-butyl, n and cyclohexyl) , alkyl amine (primary and secondary) ,-NMe 2 , MHMe, - (CH 2 ) n NMe 2 ,- (CH 2 ) n NHMe, -(CH 2 ) n NH 2 , alkoxy [0(CH 2 ) 2n CH 3 ) n ] , aromatics (-C 6 HR 7 wherein R 7 is H, NH COOH, NCS, CHO, activated esters, acid anhydrides, (N-hydroxy succinimide, and SiMe 3 . R 2, R 3 , R 4 , R 6 are all the same or different being H, alkyl (Me, Et, n-or i- propyl, n-, i-or t-butyl, n- and cyclohexyl) , alkyl amine, primary and secondary [-NMe 2 , NHMe, - (CH 2 ) n NMe 2 - (CH 2 ) n NHMe, - (CH 2 ) n NH 2 ], alkyoxy [0 (CH 2 ) n CH 3 or - (CH 2 ) n OCH 3 ]. R 5 is the same or different than R 2 , R 3 , R 4 , R 6 and is all the substitutes defined for X accept for the hydrazine group. E is O, S, NSiME 3 , a lone pair of electrons, NC 6 H 4 R 8 ,R 8 = H, NH 2 , CooH, NCS, CHO, N- hydroxysuccinimide, activated esters or acid anhydrides. Similar reactions of germanium halides (eg., GeCl 4 , RGeCl 3 ) with hydrazine reactants as defined above for the phosphorous core produced germanium hydrazides containing up to four hydrazine units. DETAILED DESCRIPTION OF THE INVENTION Generally, the present invention provides a compound for use as a diagnostic or therapeutic pharmaceutical however, they may also be used for other pharmaceutical applications including MRI contrast agents. The compound includes a phosphine core and at least two hydrazine groups forming a ligand for bonding to a metal extending from the phosphorous or germanium core. That is, the invention provides phosphorous and germanium hydrazide ligand systems containing between 2 and 4 hydrazine units for use in forming complexes with a variety of transition metals that have high in vitro and/or in vivo stability. The phosphorous and germanium ligands were chosen since the hydrazine arms linked directly to phosphorous atom provides a plethora of electron density on the terminal hydrazine-N-atoms that promote formation of highly stable nitrogen-metal bonds. This occurs even with the transition metals in their higher oxidation states, such as Re v . The utilization of at least two hydrazine groups for metal bonding produces complexes that are more stable than the metal complexes with only one hydrazine arm. The ligand is complexed with the transition metal, generally from the group including Fe, Mn, Re, Re, Pd, Rh, and 99m Tc. These complexes contain a 1:1 metal to ligand ratio which is formed making the resulting chelates small and well- defined. These specific combinations permit the formation of the complexes in a one step, high yield reaction as described below, especially for use with readily available chemical forms of the radionuclides. Other metals suitable for chelating are copper and cobalt. For example, 99 Tc0 4 , Re0 4 chelates or PdCl 2 can be used. It has been determined that these types of ligands form highly stable chelates with a variety of transition metals that have radioactive isotopes that have potential for formulation of new therapeutic uses, such as 186 Re, 188 Re, 109 Pd, 105 Rh, etc. , or for diagnostic use such as with 9m Tc radiopharmaceuticals. For example, Fe and Mn are paramagnetic elements and have potential application in chelate form as MRI contrast imaging agents. Stable Mn and Fe chelates with the P and Ge hydrazine ligands with appropriate substituents can be formulated. The chelates made in accordance with the present invention have been found to be stable in aqueous solutions, serum and other body fluids. This is critical to solve the problems of prior art agents which did not form stable chelates thereby having an inherent loss of control of localization of the radionuclide paramagnetic metal. Further, compounds made in accordance with the present invention can be chemically modified, as discussed below, to provide for specificity of localization, increased physical half-life of the radionuclide, improved pharmacokinetics, and increased selectivity of target tissues, such as tumors, over normal tissue, such as bone marrow, kidney, GI tract, liver etc. The compounds made in accordance with the present invention are not only stable in neutral aqueous solutions, but have also been found to be stable in acidic and basic aqueous media. Again, this is critical with regard to localization of the compound in areas of the body having different pH's, as well as being stable through different administration routes, such as oral administration. More specifically, the bis-hydrazine ligand made in accordance with the present invention can characterized by the following formula: N - R 3 Formula I 4 R -N 4 R x , R 2 , R 3 , R , are all the same or different and are H, alkyl (Me, Et, n or i propyl, n, or i butyl, n or cyclohexyl) -CH 2 COOH; -CH(OH)CH 2 OH; alkylamine (primary and secondary); -NMe 2 , NHMe, (CH 2 ) n NMe , (CH 2 ) n NHMe,-(CH 2 ) n NH 2 ; alkoxy (-0(CH 2 ) n CH 3 or (CH 2 ) n OCH 3 ; -(CH 2 ) n -COOH or - (CH 2 ) n SH; aromatics, C 5 H 5 R wherein R = H, NH 2 , COOH, OH NCS, CHO, activated esters, acid anhydrides, N- hydroxy succina ide, and SiMe 3 , R 5 = H; OCH 3 -CH(OH)- CH 2 OH, Me, Ph. E = 0, S, -NSiMe 3 , a lone pair of electrons; NC 6 H 4 R wherein R = H, OH NH 2 , COOH, NCS, CHO, N-hydroxy succinamide, activated esters or acid anhydrides; wherein n as used herein is the number 1 to 6; and Q is hydrogen -CH 2 Ph or = CHPh wherein in each instance Ph is unsubstituted or substituted with OH, COOH, 0 alkyl wherein Alkyl are from 1 to 4 carbon atoms, NH 2 , NH alkyl C x _ 4 or NH(Alkyl C^^) 2 , or halogen such or chlorine, fluorine, brome or iodine or Q can be -CH 2 piperazino or CH piperazino. The tris-hydrazine ligand made in accordance with the present invention can be characterized by the following: R 6 N /I N^ / P^ Q Formula II R x , R 2 , R 3 , R 4 , are all the same or different and are H, alkyl (Me, Et, n or i propyl, n, or i butyl, n or cyclohexyl) alkylamine (primary and secondary) ; - NMe 2 , NHMe, (CH 2 ) n NMe 2 , (CH 2 ) n NHMe, -(CH 2 ) n NH 2 ; alkoxy (-0(CH 2 ) n CH 3 or (CH 2 ) n OCH 3 ; aromatics, C 6 H 5 R wherein R = H, NH 2 , COOH, NCS, CHO, activated esters, acid anhydrides, N-hydroxy succinamide, and SiMe 3 , R 5 R 6 = H; Me, Ph. E * 0, S, -NSiMe 3 , a lone pair of electrons; NC 6 H 4 R:R = H, NH 2 , COOH, NCS, CHO, N- hydroxy succinamide, activated esters or acid anhydrides; n and Q as defined in Formula I and further each of R x to R 5 can be defined as in Formula I and R 6 can be the same as R 1 to R 5 as defined in Formula I. The germanium hydrazide ligands made in accordance with the present invention can be characterized by the following: R l f R 2 , R 3 , R , are all the same or different and are H, alkyl (Me, Et, n or i propyl, n, or i butyl, n or cyclohexyl) alkylamine (primary and secondary) ; - NMe 2 , NHMe, (CH 2 ) n NMe 2 , (CH 2 ) n NHMe, -(CH 2 ) n NH 2 ; alkoxy (-0(CH 2 ) n CH 3 or (CH 2 ) n OCH 3 ; aromatics, C 6 H 5 R wherein R= H, NH 2 , COOH, NCS, CHO, activated esters, acid anhydrides, N-hydroxy succinamide, and SiMe 3 , R 5 R 6 = Cl, Me, Ph or R 1 N-NH 2 NC 6 H 4 R; R = H, NH 2 , CooH, NCS, CHO, N-hydroxy succinamide, activated esters or acid anhydrides are all the same or different; n and Q are as defined as in Formula I; and furhter R 1 to R 6 can be defined as in Formula II. The above formulas characterize the present invention as being very modifiable in order to specifically tailor the ligand for chelation with a specific radionuclide and localization at a specific target organ. For example, the ligand can be conjugated to protein or antibodies and can use side chains previously used for linking monoclonal antibodies (15) . For example, conjugation reactions can involve reactive groups such as benzyl isothiocyanate, bromoacetamide, activated esters, N- hydroxysuccini ides, cleavable ester linkages, and aldehydes (15) . In the case where E is a lone pair of electrons on the phosphorous atom, attachment of the chelate or the hydrazide ligand to proteins or other molecules already containing an azido group can be brought about by the standinger reaction. The conjugation reaction can occur using any of the R groups attached to the nitrogens on one of the hydrazine side arms or attached directly to the phosphorous or germanium atom. Accordingly, a single monoclonal antibody or several monoclonal antibodies can be added to the phosphorous or germanium hydrazine core to provide specificity of the binding of the ligand metal complex to specific surface antigen of target tissue. As discussed above, other side chain modifications can be accomplished to make the chelate more polar and hydrophilic. For example, charged groups such as carboxyl or hydroxyl groups can be added at the various R groups appended to the hydrazine nitrogens. This additional small change in the compounds providing charged/polar groups increases the hydrophilic character of the resulting chelate. This will produce more rapid and selective clearance from the blood and nontarget tissue. This modification is highly desirable for the promotion of efficient clearance of radioactivity from nontarget tissues, such as blood, liver, kidney, and spleen following catabolism of conjugated radiolabeled monoclonal antibodies that are presently used for therapy. Alternatively, the hydrophobicity of the chelate can be varied incrementally by varying the alkyl chain length of the side chains appended to the hydrazine nitrogens. For example, the R groups or the hydrazine side arms can be derivatized with hydrogen, methyl, ethyl, n-, or -i-propyl or ni-, or t-butyl. This is desirable because with some chelates, particularly those labeled with 99m Tc, an increase in the hydrophobicity of the chelate plays a major role in targeting uptake in selective tissues, such as in brain, heart and lung. Addition of alkyl groups to the chelating backbone increases the lipid solubility of the chelate. If the resulting chelate is neutral, either brain, heart, or lung imaging agents can be developed (18) . Similarly, if the overall chelate charge is +1 myocardial imaging agents can be developed (19) . Modifying the hydrophobicity of chelates with beta- emitting radionuclides for therapy will also change the clearance and uptake properties in target and nontarget tissues. An alternative to varying the alkyl chain length of the R groups appended to the nitrogens of the hydrazines is to add other hydrophobic functional groups, such as alkyl methoxy and alkyl methoxys to the R groups. The use of ether side chains instead of the alkyl side chains will increase lipophilicity but also improves the rate of clearance of the chelate from the blood and other non-target tissues. Other side chain modifications to increase hydrophilicity, such as the addition ester groups or amide groups, can also be accomplished. Another alternative modification of the chelate is to modify the charge or basic metal chelate core. It is possible to make the chelate with zero charge to produce a neutral hydrophilic chelate. For example, the bis-hydrazine ligand can be modified by the addition of a side chain other than hydrogen at the R groups attached to one of the hydrazine terminal nitrogens. Alternatively, negatively charged groups can be added, such as (-CH 2 ) n -SH or(-CH 2 ) n -C00H. All of the aforementioned modifications demonstrate the flexibility of compounds made in accordance with the present invention and further the ability to modify these compounds to alter the binding, elimination, and absorption of the compounds in order to tailor the compounds for specific organ targeting, dosing, and metabolism. Compounds made in accordance with the present invention for use as diagnostic or therapeutic pharmaceuticals can be made by the following general reaction: t wherein R x = H; X is N-N-H, alkyl, (Me, Et, n-or i - propyl, n-, i-or t-butyl, n and cyclohexyl) , alkyl amine (primary and secondary) ,-NMe 2 , MHMe, - (CH 2 ) n NMe 2 ,- (CH 2 ) n NHMe, -(CH 2 ) n NH 2 , alkoxy [0(CH 2 ) n CH 3 ) n ], aromatics (-C 6 H 4 R 7 wherein R 7 is H, NH COOH, NCS, CHO, activated esters, acid anhydrides, (N-hydroxy succinimide, and SiMe 3 . R 2 ' R 3 , R 4 , R 6 are all the same or different being H, alkyl (Me, Et, n-or i- propyl, n-, i-or t-butyl, n- and cyclohexyl) , alkyl amine, primary and secondary [-NMe 2 , NHMe, - (CH 2 ) n NMe 2 - (CH 2 ) n NHMe, - (CH 2 ) n NH 2 ], alkyoxy [0 (CH 2 ) n CH 3 or - (CH 2 ) n 0CH 3 ] . R 5 is the same or different than R 2 , R 3 , R 4 , R 6 and is all the substitutes defined for X accept for the hydrazine group. E is 0, S, NSiME 3 , a lone pair of electrons, NC 6 H 4 R 8 ,R 8 = H, NH 2 , CooH, NCS, CHO, N- hydroxysuccinimide, activated esters or acid anhydrides. A solution of the appropriate phosphorous halide (e.g. P(0)C1 3 , P(S)C1 3 , PC1 3 ; RPC1 2 R=Ph,Me...) in THF, toluene or chloroform was mixed with the solutions of lithiated or silyated hydrazines according to a set stoichiometry at 25°C. The reaction mixture was stirred at RT for 8-16 hours before the solvent was removed in the vacuum. The purifications of the phosphorous hydrazides were achieved either by recrystallization or through distillation under reduced pressure. A solution of GeCl 4 or RGeCl 3 (R=Me,PH) in THF, toluene or CHC1 3 was mixed with the solutions of lithiated or silyated hydrazines according to a set stoichiometry at 25 β c. The reaction mixtures were stirred at RT for 8-16 hour before the solvent was removed in vacuum. The germanium hydrazides were purified either by recrystallization or through distillations at reduced pressure. The following are examples of ligands and chelates formed in accordance with the present invention. -20- EXAMPLE 1 Ligands Synthesized 1 2 10 R ^ X =_ N — N, Ph, Me R χ = R2 = R 3 = R 4 = H ; R L = R 4 = Me E = O, S, NSiMe or electron pair 15 R = C1; NMe — NH 2 ; Ph; Me EXAMPLE II Formation of Complexes with Rehenium 1. Re-BHP Complexes formed: Complex 1^ ) Characterized by l H and 31 P NMR and H^Ϊ elemental analysis b. Cpd H^, »H, 31 P NMR QH,N EL AnaL -22- c. Foπnation of THP complexes with Re. S I OSiMe 3 d. Foπnation of BHP complexes with Re Cpd lV^,, Η, 31 P NMR QH,N EL Anal For the following examples unless otherwise stated, all reactions were carried out under anaerobic and anhydrous conditions using prepurified N 2 and conventional Schlen techniques. Reagents such as CO(C10 4 ) 2 6H 2 O, Cu(C10 4 ) 2 6H 2 0, P(S)PhCl 2 and PdCl 2 were purchased from Aldrich Chemical Co., USA and were used without further purification. Phenylphosphodihydrazide 1 was prepared by the reaction of PhP(S)Cl 2 with methyl hycurazine. (9,10). Nuclear magnetic resonance spectra were recorded on a Bruker WH-500 Spectrometer. The 1 H NMR chemical shifts are reported in parts per million (ppm) downfield from external standard SiMe 4 . The 31 P NMR spectra were recorded with 85% H 3 P0 4 as an external standard and positive shifts lie downfield of the standard. The structure of the compounds prepared are set forth in Equation (1) and Schemer 2 to 4 hereof.\ Synthesis of Complex 2: To a solution of 1(4.7 g; 20.25 mmol) in absolute ethanol (lOOmL) was added dropwise with stirring at 0°C a solution of Co(C10 4 ) 2 6H 2 0 (2.47g; 6.74 mmol) also in absolute ethanol (50mL) . Upon completion of addition (30 minutes) , a pink solid precipitated out. The mixture was stirred at 25°C for 6 hours before the solid precipitate was filtered and dried in air to obtain shiny light pink crystalline solid of analytically pure 2 (yield 3.93g; 95% based on Co(C10 4 6H 2 0) ; mp 180°C dec. Anal. Calcd. for C 16 H 30 N 8 CIO 4 P 2 S 2 CO: C, 31.04; H, 4.85; N, 18.11; Cl, 573. Found: C, 31.02; H, 4.87; N, 18.10; Cl. 575. Synthesis of Complex 3: To a solution of 1 (4.53g_ 19.70 mmol) in THF (150 mL) was added dropwise (30 minutes) with stirring at 0°C a solution of Cu(cl0 4 ) 2 6H 2 0 (1.82 g; 4.92 mmol) in absolute ethanol (50 mL) . The mixture on stirring at 25°C for 8 hours turned greenish with suspensions of similar colored solid precipitate. The solvents were removed under vacuo and the crystalline residue was washed successively (4 x 25 mL) the THF to remove the unreacted excess of 1. The leftover green solid was found to be analytically pure 3 (yield 2.80g; 91% based on Cu(C10 4 ) 2 6H 2 O) ; mp 194C dec. Anal. Calcd for C, 30.81; H, 4.81; N, 17.97; Cl, 5.69. Found: C,30.84; H, 4.83; N, 17.96; Cl, 5.71. Synthesis of Complex 4: A solution of PdCl 2 (PhCN) 2 (3.53g; 9.22 mmol) in CH 2 C1 2 (100 mL) was added dropwise (15 minutes) at 25°C to a solution of 1 (2.12g; 9.22 mmol) also in CH 2 C1 2 (100 ml) . The dark orange colored mixture on stirring for 4 hours turned yellow and the solvent was removed in vacuo to obtain a brown colored icrocrystalline solid which upon washing with THF (2 x 10 ml) gave analytically pure 4 (yield 3.42g; 91%) mp 210°C dec. Anal, calcd. for C 8 H 15 N 4 Cl 2 PSPd: C, 23.56; H, 3.68; N, 13.74; cl, 17.40. Found: C,23.54; H, 3.70; N, 13.71; Cl, 17.51. Synthesis fo Compound 5: To a solution of 1 (3.50g; 15.26 mmol) in absolute ethanol (100ml) was added dropwise (15 minutes) at 25 β C with stirring a solution of salicyladehyde (3.81g; 31.28 mmol) in absolute ethanol (100 ml). The mixture was stirred under reflux for 12 hours before the solvent was removed under vacuo to obtain a white crystalline sold of 5 which was recrystallized from boiling acetonitrile (yield 6.4g; 96%); mp 80°C Anal. Calcd for C 22 H 23 N 0 2 PS: C, 60.22; H, 5.25; N, 12.78. Found C,60.21; H, 5.21; N, 12.74. Synthesis of Compound 6: was synthesized by the reaction of 1 with piperazine aldehyde under identical reaciton conditions as described above for 5. Recrystallization from CH 3 CH/CH1 3 (3:1) (yield 88%) mp 109 β C. Anal. Calcd for C 18 H 31 N 8 PS: C, 51.11; H, 7.34; N, 26.52. Found: C, 51.14; H, 7.37; N, 26.50. - 26- Synthesis of Complex 7: to a solution 5 (2.95g; 6.73 mmol) in THF (100 mL) was added wi stirring at 25°C a solution of PdCl 2 (PhCN) 2 (2.58 6.73 mmol) also was removed under vacuo to obtain 5 brown microcrystalline solid of 7. The crude 7 w washed with chilled CH 2 C1 2 (2 x 10 mL) to remove t residual benzonitrile before it was recrystallize from acetonitrile (yield 3.60g; 92%); mp 151°c (dec). Anal. Calcd for C 22 H 22 N 4 C10 2 PSPd: C, 45.64; 10 H, 3.83; N, 9.67; Cl, 6.12. Found: C, 45.57; H, 3.84; N, 9.70; Cl, 6.10. Synthesis of Complex 8: To a suspensio of 6 (2.75g; 6.51 mmol) in dichloromethane (50 mL) was added with stirring at 25°C a solution of 15 Pdcl 2 (PhCN) 2 (2.49g; 6.51 mmol) also in dichloromethane (50 mL) . The mixture was stirred for 6 hours before the solvent was removed in vacu to obtain an orange colored microcrystalline solid of 8. Recrystallization from boiling CH 3 CN gave 20 analytically pure 8 (yield 3.25 g; 83%); mp. 163°C(dec). Anal. Calcd for C 18 H 31 N 8 Cl 2 PSPd: C, 36.02; H, 5.17; N, 18.67; Cl, 11.82. Found: C, 36.11; H, 5.15; N, 18.63; Cl, 11.84. 25 The invention has been described in an illustrative manner, and it is to be understood that the terminology which has been used is intended to be in the nature of words of description rather than of limitation. Obviously many modifications and variations of the present invention are possible in light of the above teachings. It is, therefore, to be understood that within the scope of the appended claims, the invention may be practiced otherwise than as specifically described. Equation (1) Sc eme 2 M(Clo 4 ) : 6H 2 O M = Co, 2 ; Cu,3 Sche e 3 Scheme 4 PdCI 2 (PhCN) 2 H

Claims: What is claimed: 1. A compound for use as a diagnostic or therapeutic pharmaceutical, or MRI contrast agent said compound comprising: a phosphine core, and at least two hydrazine groups forming a ligand for binding to a metal extending from said phosphorous core. 2. A compound as set forth in claim l wherein said ligand is a bis-hydrazine phosphine. 3. A compound wherein said ligand is of the formul a: R lf R 2 , R 3 , R 4 , are all the same or different and are H, alkyl (Me, Et, n or i propyl, n, or i butyl, n or cyclohexyl) alkylamine (primary and secondary) ; - NMe 2 , NHMe, (CH 2 ) n NMe 2 , (CH 2 ) n NHMe,-(CH 2 ) n NH 2 ; alkoxy (-0(CH 2 ) n CH 3 or (CH 2 ) n OCH 3 ; aromatics, C 6 H 5 R wherein R = H, NH 2 , COOH, NCS, CHO, activated esters, acid anhydrides, N-hydroxy succinamide, and SiMe 3 , R 5 = H; Me, Ph. E = 0, S, -NSiMe 3 , a lone pair of electrons; NC 6 H 4 R:R=H, NH 2 , COOH, NCS, CHO, N-hydroxy succinamide, activated esters or acid anhydrides. 4. A compound as set forth in claim l wherein said ligand is a tris-hydrazine phosphine. 5. A compound as set forth in claim 4 wherein said ligand is of the formula: R l t R 2 , R 3 , R 4 , are all the same or different and are H, alkyl (Me, Et, n or i propyl, n, or i butyl, n or cyclohexyl) alkylamine (primary and secondary) ; - NMe 2 , NHMe, (CH 2 ) n NMe 2 , (CH 2 ) n NHMe,-(CH 2 ) n NH 2 ; alkoxy (-0(CH 2 ) n CH 3 or (CH 2 ) n OCH 3 ; aromatics, C 6 H 5 R wherein R = H, NH 2 , COOH, NCS, CHO, activated esters, acid anhydrides, N-hydroxy succinamide, and SiMe 3 , R 5 ,R 6 = H; Me, Ph. E = 0, S, -NSiMe 3 , a lone pair of electrons; NC 6 H 4 R:R=H, NH 2 , COOH, NCS, CHO, N-hydroxy succinamide, activated esters or acid anhydrides. 6. A compound as set forth in claims 3 or 5 wherein said ligand is complexed with a transition metal. 7. A compound as set forth in claim 6 wherein said transition metal is a radionuclide selected from the group including 186 Re, 188 Re, 109 Pd, 10S Rh and 99m Tc, said compound being stable in aqueous solutions, serum and other body fluids. 8. A compound as set forth in claim 7 wherein said transition metal is a paramagnetic transition metal selected from the group includes Fe or Mn, said compound being stable in aqueous solutions, serum or other body fluids. 9. A compound as set forth in claims 7 or 8 including a 1:1 metal to liqand ratio. 10. A compound as set forth in claims 3 or 5 wherein said ligand is conjugated to a protein or antibody. Page missing at the time of pubiication 15. Compounds wherein ligands are of the formul : R 5 R 5 Ge R lf R 2 , R 3 , R 4 , are all but the same or different and are H, alkyl (Me, Et, n or i propyl, n, or i butyl, n or cyclohexyl) alkylamine (primary and secondary) ; -NMe 2 , NHMe, (CH 2 ) n NMe 2 , (CH 2 ) n NHMe, -(CH 2 ) n NH 2 ; alkoxy (-0(CH 2 ) n CH 3 or (CH 2 ) n OCH 3 ) wherein n is l to 6; aromatics, CH 6 H 5 R wherein R = H, OH NH 2 , COOH, NCS, CHO, activated esters, acid anhydrides, N- hydroxy succinamide, and SiMe 3 , R 5 , R 6 = Cl, Me, Ph or R 1 N-NH 2 NC 6 H 4 R; R = H, NH 2 , COOH, NCS, CHO, N- hydroxy succinamide, activated esters or acid anhydrides; are all the same or different; Q is H, - CH 2 Ph or =CHPh wherein Ph is unsubstituted or substituted with OH, COOH, -O alkyl wherein alkyl has from 1 to 4 carbon atoms, -alkyl(CO-6)- NY2 wherein Y and Z are H or alkyl C x . 4 or Ph is substituted with halogen, or Q is -CH 2 piperazino or =CH piperazino. 16. A method of making a compound for use as a diagnostic or therapeutic pharmaceutical, said method including the following reaction: Cl E=P—Cl \ X R 5 R 6 wherein R x = H; X is N-N-H, alkyl, (Me, Et, n-or i - propyl, n-, i-or t-butyl, n and cyclohexyl) , alkyl amine (primary and secondary) ,-NMe 2 , MHMe, - (CH 2 ) n NMe 2 ,- (CH 2 ) n NHMe, -(CH 2 ) n NH 2 , alkoxy [0(CH 2 ) 2n CH 3 ) n ] , aromatics (-C 6 H 4 R 7 wherein R 7 is H, NH 2 COOH, NCS, CHO, activated esters, acid anhydrides, (N-hydroxy succini ide, and SiMe 3 . R 2 ' R 3 , R 4 , R 6 are all the same or different being H, alkyl (Me, Et, n-or i- propyl, n-, i-or t-butyl, n- and cyclohexyl) , alkyl amine, primary and secondary [-NMβ 2 , NHM«, - (CH 2 ) n NMβ 2 - (CH 2 ) n NHMβ, - (CH 2 ) n NH 2 ], alkyoxy [0 (CH 2 ) n CH 3 or - (CH 2 ) n 0CH 3 ]. R 5 is the same or different than R 2 , R 3 , R 4 , R 6 and is all the substitutes defined for X accept for the hydrazine group. E is 0, S, NSiME 3 , a lone pair of electrons, NC 6 H 4 R a ,R 8 - H, NH 2 , CooH, NCS, CHO, N- hydroxysuccinimide, activated esters or acid anhydrides. 17. A method as set forth in claim 18 further including the step of chelating the compound to a transition metal selected from the group including Fe, Mn 186 Re, 188 Re, 109 Pd, 105 Rh and 99m Tc, said compound being stable in aqueous solutions, serum and other body fluids. 18. A method as set forth in claim 16 wherein said compound is conjugated to a protein or antibody. 19. A method as set forth in claim 18 further including the step of derivatizing R 1 , R 2 , R 3 , R 5 , or R 6 , to a benzyl isocyanate, bromoacetamide, activated ester, N- hydroxysuccinimide, cleavable ester, or aldehydes. 20. A method as set forth in claim 16 further including the step of modifying R 1 , R 2 , R 3 , R 5 , or R 6 , to be more polar, thereby rendering the compound more hydrophilic. 21. A method as set forth in claim 20 wherein said modifying step is further defined or hydroxylating or carboxylating R 1 , R 2 , R 3 , R 5 , or R 6 . 22. A compound of claim 1 having the formula wherein E is =0, =S, -NSiMe 3 , a lone pair of electrons; NC 6 H 4 R wherein R is OH, H, NH 2 , COOH, NCS, CHO, N-hydroxy, succinamide, activated esters or acid anhydrides; wherein R 5 is H, 0CH 3 , -CH(OH)- CH 2 OH, CH 3 , Ph; wherein R 1 R 2 , R 3 , R 4 and R 6 are the same or different and are H, straight or or branched cyclohexyl; -CH 2 C00H; -CH(0H)CH 2 0H; alkyl(C 0 _ 6 ) NYZ wherein each of Y and Z is hydrogen or lower alkyl 0.^-C L ; straight or branched alkoxy having from 1 to 6 carbon atoms, -(CH 2 ) n - 0CH 3 wherein n is l to 6, phenyl or phenyl substituted with OH, NH 2 , COOH, NCS, CHO, activated estes, acid anhydrides N- hydroxysuccinamide, SiMe 3 ; Q is H, -CH 2 Ph or = CHPh wherein each Ph is unsubstituted or substituted with OH, COOH, Oalkyl wherein alkyl has from 1 to 4 carbon atoms, alkyl (C 0 _ 6 )NYZ wherein Y and Z are as defined above or pH is substituted with halogen, or Q is -CH 2 piper = azino or =CH piperazino.

Inventors: KATTI KATTESH V, KETRING ALAN REED, VOLKERT WYNN ARTHUR, KATTI, KATTESH, V., KETRING, ALAN, REED, VOLKERT, WYNN, ARTHUR
Applicants: UNIV MISSOURI, THE CURATORS OF THE UNIVERSITY OF MISSOURI
Application year: 1992
Main classification: C07C
IPC codes: A61K51, C07F7, C07F9, C07F13, C07F15, C07F19