A PROCESS FOR DE-POLLUTION BY MEANS OF BIODEGRADATION AND MEANS FOR PERFORMING THE SAME

Abstract: A process for the purification, by means of biodegradation and mineralization, of organic polluting substances, due to the synergic action between natural sea bacteria that have not been genetically manipulated, and a pseudo-enzymatic bio-catalyst, consisting of various elements, called bio-reactor. The preparation of the obliged aerobic bacteria takes place by cavitation with a magnetic dragging pump and particular means for dishydrating and putting into microcapsules the ''nourishing'' so as to allow a slow and delayed release in the surrounding ambient.

Description: "A PROCE SS FOR DE-PO LLUT I ON BV ME ANS OF BIODEGRADATION AND MEANS FOR PERFORMING THE SANE" The present i nventi on concerns a de-pollution process by means of biodegradation and the means for performing the same, like natural sea bacteria. The problem of pollution is considered the worst mankind must afford and solve in these last years of the 20th century. It has already showed up in the past when, for making determi ned areas i nhabitable, man had to consi derabl y disturb the pre-existing conditions and balances. But this had occurred graduall y, foll owing to the population's development: the agricultural transformations took place sl owly, with the possibili ty of a gradual ec ol og i cal adaptation; the building and industrial activiti es were limi ted and consequently also the resulting pollution was limited and the solid waste, spread on the soil or earthed, were quickly degraded. In the past, it has been possibl e to obviate to the polluti on produced in urban areas or by industrial installations by the dilution of the wastes into rivers or seas where they were transformed, due to the action of aerobic and anaerobic micro-organisms, into harmless products. But in the last decades the quick increase of the world popul ation and the paral lel development of the industri al acti vities hav e produced strong concentrations of domestic and industrial waste. To this kind of pollution another kind must be added, deriving from local catastrophies, like the dispersion into the ambient of Seveso of 'dioxine " , the outflow of raw oil in the Alaska Gulf, in the Gulf of Mexico, in the Persian Gulf and in the Mediterranean Sea near Genoa; li ke the radi oactive contami nati ons due to the fi re i n the el ectronuclear Chernobyl-central, etc. Therefore the old system of dilution in the waters and of the spontaneous biodegradation is no longer effective due to its sl owness that does not allow to make the enormous waste mass produced in continuity, harmless. Sci entifical and technological researches onto the use of natural bacteria are actually mainly aiming to the utilization of bacteria in drinking water. The process according to the present invention is based onto the use of natural sea-water bacteri a, that are not genetically manipulated and not pathogenous, in selectioned stocks and made even more acti ve in relati on to the speci fi cal substances to demol ish and theref ore to mineralize. It is well known that i n the sea are living, beyond others, ol eophi lous, lipophagous, l ipolytical and hydrophobi cal bacteria that are natural ly destined to the metabolization and biodegradation of various oily ad organic substances. Relating to the degradative specificity of the lipidic products, above menti oned bacteri c "pools" are di fferent i n th e metabol ization speed of the organi c carbon contai ned therein. Particul arly the oleophi lus bacteri a are able to take nourishment from any organic molecule - of animal, mineral or vegetal origin - if only together with carbon, also azote and phosphorous are equally balanced, as they are nutrients indispensable to most marine micro-organisms. However, this capacity that could seem to be an advantage, shows to be the contrary when these bacteria are called to destroy mineral lipo-molecul es, as said stocks are not specific and therefore rather slow in their processes. The lipophagous bacteria, even if not being specifi c for the degradation of raw oil, have - for this purpose - better features than the oleophi lus bactera, as they make use, as a carbon source, only of those lipidic molecules containing it. Ths features makes its demoli tion metabolism somewhat quicker. For what concerns the specifici ty, for sure the l ipol ytic bacteria are more effective, as they have the capacity of selecting the organic molecules from whi ch they can take their nourishment; infact, the are not abl e to destroy any lipid - of animal , vegetal or mi neral origi n -, but each specific stock metabolizes the ones ot the others. The optimum is represented by the hydrophobi c bacteria which, due to their ciliar cytoplasmatic structure, get stuck to the l ipidic surface transforming the same, i n the "interfacies" with the water, into tri- . di- and monoglycerids, unsoluble fat acids that are a natural component of the food chain. This makes their specificity, as well as the enormous reproduction quickness (about twenty minutes), evident and this quickness all ows them al so to destroy the most complicated lipidic chains. The qualification of above mentioned bacteria, call ed "marine', intends to underline their "specific love" for the sea-water ambients, whi le the qualification of 'natural' wants to underline that they li ve i n nature, speciall y in those areas of the globe that have suffered from ecological disasters during millennia - like the breaking of oil streams and the fol lowi ng outfl ow of raw oil - caused by earthquakes and/or tectonic landslides, to which the same nature has provided remedies (natural bio-remediati on) but with very long times. Therefore, it is obvious that for the reducti on of such bacteria it is not necessary to appeal to genetic engeneering techniques, as the different stocks - mainly Pseudomonas - are found in above mentioned areas, are i sol ated with appropriate micro-biological methods based on the use of selected culture grounds, and classified according to thei r particular features, and thus they will form the base of the bacteriotheque from which single bacteria will be taken for the preparation of the speci fic bacteric societi es needed (said "societies' consist in a plurality of "pools" that are in turn formed by groups of bacteria stocks having si milar features for what concerns degradation). So as to make the bacteria reproduce in the chosen stock, quickly and in excess, and so as to collect the bio-mass when that stock is prevailing on others, it is necessary to create an appropriate ambient by means of particular apparatuses, and then to fix the bio-mass onto an atoxic substratum with a neutral ph of inorganic nature, like betonite. Foll owing to such treatment the bio-mass becomes pul verulent and/or organic, like vegetal starch. If the bacteric powder is used in marine or lake ambi ents, it should be additioned wi th nutrients of phosphorous and azote base, put into atoxic paraffin microcapsules, that belong to the main features of the present invention. By means of the use of appropriate apparatuses, it has shown to be possible to realize the production process i n laboratory, based - according to the present invention - onto a development due to specific fermentati on of a broth- culture (the most appropri ate for the growth of the requested stock), mainly consisting in bacteriologically pure sea-water (if necessary previously steri lized), having a temperature around 28°, additioned with nutrient salts (not being micro-incapsulated) and of products able to neutralize the toxic-harmful metals eventuall y present in the water solution. Furthermore, it is necessary to give the bacteria the organic carbon indispensable to their growth, that usually is present in raw oil, while the oxygen is provided by a liquid bio-catalyst poured into the fermentator and by a particular "cavi tation" system. If necessary, the ph of the broth- culture, that must be sl ightl y basic, wi ll have to be corrected, according to the situations, with acidi ficators or alkalifiers. Practically, the production process is actuated by means of a hydraulic system shown i n figure 1 , out of plastic atoxic materi al, i n which the broth-cul ure i s circulating, and mainly consisting of: 1 ) - a fermentator containing the broth-culture; 2) - a magnetic dragging pump with a "soft" propeller; 3) - a bacteric anti-shock. The fermentator may be with superficial getting 1 or ground getting 2; in the first case the broth-culture is aspired to the surface by means of a pump that re-introduces the same into the low part of said fermentator (figure 1 ), giving the bio-mass a hydrodynamic push and a consequent whi rli ng motion favoured by the funnel shape of the invitation 3 placed at the centre of the fermentator. In the second kind, the broth-culture i s aspired from the bottom and re- introduced to the surface; in this case also a whirling motion is created that in turn causes an aerobi c peri s tal ti c cavitation phenomenon similar to the one that occurs in the fermentator of the first kind, but without arrhythmy, to as to maintain said broth-culture constantly air saturated. The main dimensional features that are equal for both kinds of fermentators, are the following: a) a volume comprised between 100 and 1 ,000 litres, b) a diameter comprised between 0.50 and 1.00 meter, c) a height minor or equal to twice the diameter. The circulation pumps of the broth-culture must have two main features: they shall not transmit heat to sad broth- culture and they shall not cause the breaking of the bacteric cel l membranes. Therefore magneti c dragging pumps are beli eved to be more appropriate, wi th low revolution number - however not above 4,000 r.p.m. -, low manometric preval ence and an open kind impeller, preferably hand- turned. The metabolic processes of the marine micro-organisms may be catalized - as has been di scovered - by a particular substance which has been given the name of "bi o-catal yst" having a pseudo-enzymatic acti on as, even not beeing an enzyme, it has all features of the same. It shows up in the shape of microni∑ed powder completely lacking pathogenous mi cro-organisms, and consists of a mixture of various elements each of which gives the bio-catal yst particular features. The main feature consi sts in the capaci ty of producing risi ng oxygen of bi ologic nature due to some photochemiosynthetical micro-organisms living therein, the same who three and a half bil lion years ago gave thei r primordial oxygen contribution to the atmosphere creating the conditions that would have al lowed, years later, the evolution of more complex living forms and that still today, together with the plants, help to maintain the air mixture composition constant. This synergy prooves to be particularly important when the marine bacteria are to be used i n the depurative processes as, beyond the accelerati on of the metabolism of sai d processes, said synergy is a real protection for said bacteria as it makes them less sensible to the salinity variations and therefore makes possible their use also in fresh water (salt- tolerance). Furthermore, the hyperactivation of he bacteria due to the bio-catalyst eliminates the bad smells in the depuration processes, all ows the reduction of the muds volume and prevents the "balcking" effect. The other details of the figure show: - pumps 6 for the rising of the mess waters; - the bacteria dosage 7; - the drainage pump 8; - the way to the sewage 9. For the production of the bio-catalyst, the present invention provides the use of an apparatus shown in figures 2a and 2b, completely out of atoxic material, having a cylindrical shape 10 and a diameter vari able between 0.40 and 1 .20 meters and a height comprised between 4.00 and 6.00 meters. At the top of said column a weir 9, a col lection channel , a vertical tube 1 1 having a di ameter of 10- 12 centimeters and a length of 1.00 to 1.50 meters, and a cover 12 are placed. Into said tube 1 1 an anhydrous catalyst together with micronized water will be introduced, and the falling thereof will be checked by a band propeller (D.N.A. configuration) or by an overturned roof system. For starting a fermentator a well determined process must be followed: first of all, the bio-catalytic powder must be hydrated with a l iquid bio-catalyst, and the column to be activated must be filled up with this mixture for the height of at least one foot. The moisturized material will be placed into a container for fermentation, and said container is out of atoxic material, sealed, placed in a dark ambient and kept at a constant temperature for a period of two weeks. At the same time, the column must be fi lled up with drinking water or in any case wi th water in whi ch no toxi ns like atra∑ine, pesticides or heavy metals are present performing, where it should be necessary, a previous filtering and de¬ toxification on acti ve carbon. After seven days the column will be emptied and then fil led up again, thi s time wi th warm water for neutralizing the eventual presence of watersoluble toxins along the walls of said column. For the warming up of the water a seal ed resistance may be placed onto the bottom or a resistance combined with a mixer may be used. Also this treatment lasts a week, and when this week has passed the column will be emptied again, filled up again for 2/3 of its height - always with detoxified water - and into said water the humidified and fermentated catalyst is poured; when the catalyst has foul ed for 25-30 minutes, new water is added up to the edge of the weir. The time requested for this treatment is a week in stand-by. Before delivering the feeding capacity to the column devellopping a bio-catalyst (C.S.B.), it should be made sure that the process has started introducing an apposite instrument into the column consisting of a tetrahedron of small zinc rods and a metal net; the outcome of gaseous bubbl es from the bottom of the column and mainly consisting of oxygen produced by 'rodospirillacee" and other photochemiosyntheti c micro-organisms present in the sediment substrate, usually shows that the process has started; it is also necessary to analise the composition of the gas each week. The sediment substrate must be renewed every day by means of an anhydrous catalyst i ntroduced into the propeller tube inside the column. The synergic combination of the bi o-catalyst wi th the marine bacteria makes it possible to intervene successfully in many different depuration and depollution processes. On the practical pl ane, the bacteria must be brought i nto contact with the materials to be degradated and that must be placed in a water solution. The specific tecnologies are diversifi ed according to the matter to be depured; e.g. if sewage waters are brought to a treatment plant, the bacteri a will be poured i nto the oxydation basin; if the problem is a leaking in the sea of raw oil, it will be necessary to sprinkle bacteric powder onto the spot formed on the surface, integrated wi th insidpensable and specific nutrients appositely placed into micro-capsules. This process has prooved to be opti mal for the maximum reduction of the marine depollution times. Infact, for quickly starting the bacteric action, they must be enriched with nutritive sal ts, placed in micro-capsules, with azote and phosphorous and in perfect balance; in this way, they are gradually giving away to the oil-water 'inter-facies', and so on. In particular, the activati on of the bacteria by means of above descri bed process may also be performed i n depuration plants, by means of the i nstall ati on of fermentators and C.S.B.

Claims: CLAI MS 1. A process for de-pollution by means of bi odegradati on and means for performing the same, based onto the development due to specific fermentations in a broth- culture consisting of bacteriologically pure marine water, at a temperature of about 28°, additioned with nutritive salts havi ng for base azote and phosphorous and wi th products for the neutralization of eventual toxic and harmful metals, characterized in: - a hydraulic plant of atoxic material, - one ore more fermentators ( 1 ) contai ning the broth- culture, and pumps (4) for the magnetic dragging and with a bacteric anti shock impeller, - a C.S.B. ("colonna svil uppante bi ocatal i∑∑atore", a bio- catalyst devellopping column) from which a watery bio- catalyst is generated following to the fermentation of the anhydours catalyst previously poured into said column. 2. A bacteric powder derived from the process accordi ng to claim 1, characterized in that it is enriched with nutritive elements based on azote and phosphorous placed into micro- capsules of atoxic paraffin for use on water surfaces. 3. A de-pollution process by means of biodegradation and means for performing the same accordi ng to clai m 1 , characterized in a system having a cyli ndrical shape ( 10) with a di ameter between 0.40 and 1.20 meters and a height between 4.00 and 6.00 meters, with a weir, a channel for the collection in a vertical tube with a diameter of 10 to 20 centimeters and a length of 1.00 to 1.50 meters, provided at the top of the column so that into said tube ( 1 1 ) the anhydrous catalyst together with sprayed water is poured, and the fall thereof is checked by a band propell er or by an "overturned-roof" system. 4. A process for de-pollution by means of bi odegradati on and means for perf orming the same, consisting of the following stages: - starting of the fermentator with the hydrati on of the biocatalytic powder, with liquid bio-catal yst and filling up with this mixture of one part of the column to be activated; - the humidified material is placed into a contai ner for fermentation, and said container is of atoxic material and sealed, placed in a dark ambient and kept at a constant temperature for two weeks; - the column will be filled up with fresh water or with water free from toxins like atrazine, pesticides and heavy metals performing, if and where necessary, a previous filtering and detoxification on active carbon; - after seven days, the column will be emptied and filled up again with warm water for neutral izing the eventual presence of water-soluble toxins along the walls; - after seven more days, the column will be emptied again and filled up again, for 2/3 of its height, with detoxified water into which the previously hydrated and f ermentated catalyst is poured; when said catalyst has fouled for 20-30 minutes, new water will be added up to the edge of the weir. 5. A process for de-pollution by means of bi odegradati on and means for perf orming the same accordi ng to the precedent claims characterized in that the checking of the start takes place introducing into the column an apposite instrument consisting of a tetrahedron of small zinc rods and a metal. net, so that the outcome of the gas bubbles from the bottom of the column and consisting of oxygen produced by "rodospi llacee" and other photochemi osynthetic mi cro¬ organisms, present in the sediment substratum, shows that the process has started. 6. A process for de-pollution by meas of biodegradation and means for performing the same accordi ng to clai m 1 , characterized i n that the sedi ment substratum must be renewed each day by means of an anhydrous catalyst introduced into the impeller tube inside the column.

Inventors: BLUNDO ROBERTO, BLUNDO, ROBERTO
Applicants: BLUNDO ROBERTO, BLUNDO, ROBERTO
Application year: 1992
Main classification: C02F3
IPC codes: C02F3