MONOCLONAL ANTIBODY AND ANTIBODY COMPONENTS ELICITED TO A POLYPEPTIDE ANTIGEN GROUND STATE

Abstract: What is disclosed is a catalytic monoclonal antibody able to cleave a polypeptide and in particular, a catalytic monoclonal antibody elicited by a ground state antigen is disclosed. Antigens include, among others, the polypeptide, a derivative of the polypeptide, a fragment of the polypeptide or any of these bound to a carrier molecule. Methods of making and using the catalytic monoclonal antibody are also disclosed.

Description: MONOCLONAL ANTIBODY AND ANTIBODY COMPONENTS ELICITED TO A POLYPEPTIDE ANTIGEN GROUND STATE FIELD OF THE INVENTION This invention relates to antibodies able to enhance the rate of chemical reaction of reactantε and in particular of peptides and polypeptides. More particularly it relates to monoclonal antibodies elicited to a polypeptide antigen and methods of making and using these antibodies. Several publications are referenced in this application by Arabic numerals within parentheses in order to more fully describe the state of the art to which this invention pertains as well as to more fully describe the invention itself. Full citations for these references are found at the end of the specification immediately preceding the claims. This application is a continuation-in-part of copending application United States patent application No. 07/498,225, filed March 23, 1990. BACKGROUND OF THE INVENTION The forces involved in ligand binding by antibodies and substrate binding by enzymes is similar, viz., hydrogen bonding, electrostatic interaction and hydrophobic effect. The energy obtained from enzyme- substrate binding may be visualized to force electronic strain in the substrate and facilitate the formation of a transition state. There is strong evidence for the theory that enzymes bind the transition state of the reaction they catalyze better than the ground state, resulting in a reduced free energy of activation for the reaction (1) . The transition state theory is based upon the observation that the reactants for a chemical reaction normally exist at a ground state energy level. In order for a reaction to proceed, the energy level of the reactants must be raised to that required to form transition state intermediate(s) . A successful catalyst may function to reduce the energy requirement for the formation of such transition state intermediate compoun (ε) . This has come to be known as the transition state theory of enzymatic catalysis. Other factors that may facilitate enzymatic catalysis include the proximity and orientation effects - apposition of correctly oriented reactants within the active site of the enzyme would reduce the requirement for a large number of random collisions prior to a productive reactant interaction. In principle, antibodies could catalyze chemical reactions by similar means. The first report of chemical conversion of a ligand by an antibody appeared in 1980 (2) , but the steroid ester hydrolysis by a rabbit polyclonal antiserum described in this report was stoichiometric rather than catalytic. Massey et al, U.S. Patent No. 4,888,281 were the first to report catalyzing a chemical reaction by means of an antibody elicited to a reactant, a reactant bound to a peptide or other carrier molecule, a reaction intermediate or analogs of the reactant, product or a reaction intermediate. Subsequently, antibodies have been demonstrated to catalyze or facilitate chemical reactions, including acyl transfer (3-6) , pericyclic (7-8) and redox reactions (9). It is generally believed that reported antibodies (3-9) obtain their catalytic properties, like enzymes, from their ability to bind the transition state of the reactant better than its ground state. Various analogs of the transition state of reactants have been used as antigens in the elicitation of immune responses (10) . The requirement for a hapten which antigenically mimics the transition state complicates efforts to obtain desirable catalytic antibodies. It would be advantageous if catalytic antibodies could be elicited to a ground state, as an antigen. Suckling et al. (11) have reported the use of a ground state antigen comprising a haptens related in structure to the substrate to elicit an antibody able to catalyze a Dielε- Λlder reaction (the addition of acetoxybutadiene to N- βubstituted maleimides) and an antibody able to cleave beta lactam rings) . Cleavage of peptide bonds by means of antibodies elicited to a selected peptide-metal complex has been demonstrated with the assistance of metal cofactors by Iverson et al. (12). Iverson et al. utilized a Co(III)triethylenetetra ine (trien)-peptide hapten in order to elicit an antibody able to accept a metal complex with chemical reactivity into the binding pocket. The discovery, isolation and characterization of naturally occurring autoantibodieε, i.e., antibodies produced by an animal's immune system to the animal's own cellular component (self-antigen) , as opposed to an antigen introduced by immunization, which enhance the rate of a chemical reaction, is disclosed in copending United States patent application No. 342,081, filed April 4, 1989. These autoantibodieε have been shown to enhance the rate of cleavage of one or more peptide bonds in vasoactive intestinal peptide (VIP) . The natural occurrence of these catalytic autoantibodies in multiple humans suggests that there is a common, naturally occurring antigen capable of eliciting these autoantibodies. Classical catalytic antibody theory suggests that the naturally occurring autoantibody must be formed in response to a high energy transition state intermediate or in response to an unusually charged analog of the peptide. It would therefore not be expected that the antigen is the ground state of a peptide, or is a large precursor protein that is eventually digested to yield the peptide, for example, pro-VIP (citation) . It is also known that antibody binding is energetically most favored by the presence of the entire H-chain and L-chain binding site (13) . The V H fragments of anti-lysozy e antibodies bind the antigen with an affinity of only 10% of the intact antibody (14) . L- chains are also likely to participate in antigen binding interactions, although most studies suggest that the contribution of L-chains is smaller than that of H-chains (15-17) . It would not be expected that an antibody component smaller than an intact catalytic antibody would possess the favorable steric conformation provided by the intact catalytic antibody to permit the catalysis of a peptide bond without the assistance of a metal trien cofactor as taught by Iverson et al. Improved antibodies and methods for selectively eliciting antibodies able to catalyze a chemical reaction of a peptide of interest are of singular interest for therapeutic products and other purposes. The use of a ground state antigen would eliminate the necessity for preparing stable analogues of transition state intermediates or haptens complexed with metal co-factors so that catalytic antibodies specific for a polypeptide of interest may be elicited as needed. There are obvious advantages that single chain proteins offer over multichain proteins (antibodies) , both from the point of view of structure-function analysis as well as pharmacological and therapeutic stability. It would be advantageous if the binding and catalytic domains on an antibody were either the same or closely positioned to one another such that the benefits of catalytic activity could be achieved by a simple protein as opposed to a multichain antibody. Heretofore, the art has not demonstrated the capability of using such components of an antibody for catalytic purposes. Similar advantages are offered by dimers formed of the several combinations of light and heavy chains. Nor has the art demonstrated that an antibody light chain elicited by a ground state reactant will have catalytic activity. OBJECTS OF THE INVENTION It is thus a primary object of this invention to provide catalytic antibodies and catalytic antibody fragments for reactions of interest using ground state antigens rather than transition state analogs to elicit an immune response. It is a further and related object of this invention to provide catalytic antibodies and catalytic antibody fragments for polypeptide reactions of interest using ground state antigen to elicit an immune response. It is still a further object of this invention to provide catalytic antibodies and catalytic antibody fragments for the hydrolytic cleavages of polypeptides of interest. It is still a further specific object of this invention to provide antibody catalyst and antibody fragment catalysts for the cleavage of specific polypeptides, e.g. vasointestinal peptide. SUMMARY OF THE INVENTION These and other objects of the invention are achieved by catalytic antibodies elicited by a ground state polypeptide antigen. In one embodiment the invention is directed to a method for catalyzing a chemical reaction of a polypeptide by a catalytic antibody wherein the catalytic antibody is elicited by an antigen comprising the polypeptide, a derivative of the polypeptide or a fragment of the polypeptide. In yet another embodiment the invention is directed to a method for catalyzing a chemical reaction of a reactant by a catalytic antibody light chain wherein the catalytic antibody light chain is derived from an antibody elicited by an antigen comprising the reactant, a derivative of the reactant or a fragment of the reactant. BRIEF DESCRIPTION OF THE DRAWINGS The invention will be understood more clearly and fully understood from the following detailed description, when read with references to the accompany figures, in which: Fig. 1 shows silver stained SDS polyacrylamide gels showing monoclonal c23.5 IgG electrophoresed under reducing conditions (lane 1) and Fab electrophoresed under non-reducing conditions; Fig. 2 shows the hydrolysis of (Tyr 20 - 125 I)VIP by increasing concentrations of c23.5 Fab estimated as the amount of TCA-soluble radioactivity; Fig. 3 shows the saturability of VIP hydrolysis by C23.5 IgG fitted to the Michaelis-Menten equation; Fig. 4 shows the hydrolysis of (Tyr 20 - 125 I)VIP by increasing concentrations of c23.5 light chains purified by gel filtration in quanidinium chloride; Fig. 5 shows the hydrolysis of (Tyr 20 - 125 I)VIP by increasing concentrations of C23.5 light chains (solid circles) recovered by electroelution following SDS- polycrylamide gel electrophoresis of IgG under reducing conditions and intact IgG (open circles) recovered by similar means following electrophoresis of the IgG under non-reducing conditions; Fig. 6 shows the anion exchange fractionation (Mono Q column) of c23.5 IgG previously purified by chromatography on immobilized protein G; Fig. 7 shows the loss of (Tyr 20 - 125 I)VIP hydrolytic activity present in fractions obtained from the anion exchange column of Fig. 6 by adsorption with immobilized anti-mouse IgG (hatched columns) wherein control adsorptions were done with equivalent amounts of immobilized anti-rabbit IgG and the results represent fractions corresponding to retention times 32-33, 34, and 35-38 minutes; Fig. 8 shows the silver stained SDS- polyacrylamide gel showing the results of non-reducing electrophoresis of fractions corresponding to retention times 32-33 (lane l) , 34 (lane 2) and 35-38 (lane 3) in Fig. 7; Fig. 9 shows the hydrolysis of (Abz)R_KQMAVKKY(N0 2 )D by increasing concentrations of C23.5 IgG estimated as the increase in fluorescence of the reaction mixtures; and Fig. 10 shows the reverse phase HPLC of (Abz)RKQMAVKKY(N0 2 )D treated with C23.5 IgG wherein identification of the peptide peaks was by amino acid sequencing. DETAILED DESCRIPTION OF THE INVENTION Definitions The term "chemical reaction" refers to a reaction wherein at least one reactant is converted to at least one product. The chemical reaction of a polypeptide may proceed by a number of different pathways, such as, for example, hydrolysis of one or more peptide bonds. The term "animal" as used herein refers to any organism with an immune system and includes mammalian and non-mammalian animals. Antibodies in accordance with the invention are elicited by presenting an antigen to immune cells. The antigen may be a protein, a polypeptide or a fragment or derivative of a protein or polypeptide either alone or coupled to a carrier. In one embodiment, the chemical reaction is the cleavage of a peptide bond. Peptide bond as used herein refers to an amide bond linking two adjacent amino acid residues and is generically represented by the following formula wherein the peptide bond is shown within the box: An amino acid consists of a carbon atom to which is bonded an amino group, a carboxyl group, a hydrogen atom and a distinctive group referred to as a "side chain" (R α and R 2 in the formula above) . Amino acid as used herein includes the twenty naturally occurring amino acids which comprise the building blocks of proteins. It is understood by those skilled in the art that when either of the adjacent amino acids is proline, the respective side chains R-L or R 2 are bonded to the adjacent nitrogen atoms to form the characteristic 5-membered proline ring. The substrate can be any proteinaceous molecule such as, for example, a regulatory protein or a structural protein, and includes, but is not limited to, peptide hormones (e.g., insulin, growth hormone, secretin, etc.), peptide neurotrans itters and neuromodulatorε (e.g., vasoactive intestinal peptide, endorphins, enkephlins, bradykinins, substance P etc.) tumor proteins (e.g., oncogene products, carcinoembryonic antigens, etc.), bacterial proteins and viral proteins (e.g., human immunodeficiency viral(HIV) gp 120, influenza glycoproteins, etc.) . Rate enhancing antibodies may in general provide rate enhancement by either catalytic or stoichiometric mechanisms. The antibodies in accordance with the invention catalytically enhance the rate of the reaction and are therefore described as "catalytic antibodies". A catalytic antibody in accordance with the invention is a substance which is capable of changing the rate of a chemical reaction, all other conditions (e.g., temperature, reactant/substrate concentration, etc.) being the same and which does not enter into the chemical reaction and therefore is not consumed in the reaction. It is also a substance which exhibits the capability of converting multiple moles of reactant/substrate per mole of catalytic antibody. From a mechanistic viewpoint the antibody binds the reactant/substrate, effects the accelerated conversion of the reactant/substrate to the product and then releases the product; and which changes the rate of the chemical reaction without shifting the position of the equilibrium. The aforementioned definitions are characteristics of ideal catalysts. However, in practice, even the best of catalysts become poisoned or deactivated by contamination in the reaction system or as a result of chemical or physical destruction during the reaction process. For reasons well known in the art, the true operation of a catalyst may be obscured by components of the reaction system or by the condition of the reaction environment. The art has adopted certain working definitions to express catalytic activity. These expressions are [1] k cat , or "turnover" and [2] the "rate enhancement factor". Turnover indicates the number of molecules of reactant/substrate which can be converted to product per mole of catalytic antibody per unit time. For example, if a molecule exhibits a turnover of 10 3 molecules of substrate per minute and the molecule maintains its catalytic activity for 24 hours at room temperature and at its optimal pH, each molecule of catalyst would then make a total of 1.4 x 10 6 conversions, indicating its catalytic behavior. This total conversion is to be distinguished from the total conversion in a stoichiometric reaction, which will never exceed 1.0, no matter how long the reaction is carried out. The rate enhancement factor is a dimensionlesε number which expresses the rate of reaction in the presence of catalyst to the rate of reaction in.the absence of catalyεt, all other reaction conditions (e.g., reactant concentration, temperature, etc.) being equal. Antibody and immunoglobulin refer to any of several classes of structurally related proteins that function as part of the immune response of an animal, which proteins include IgG, IgD, IgE, IgA, and IgM and related proteins. Antibodies are found in plasma and other body fluids and in the membrane of certain cells. Under normal physiological conditions (e.g. absent immunological dysfunction or human intervention) antibodies are produced by B cells (or the functional equivalent) of an animal in reaction to the entry of proteins or other chemical substances which that animal is not immunologically tolerant of into the tissue or body fluids of that animal. Antibody and immunoglobulin as used herein refer to any component part of an antibody. These component parts may be any fragment or other portion of a classical antibody molecule (IgG, IgA, IgE, IgD, IgM and related classes and subclasses) which retains the desired antibody function and with which the invention may be practiced. The catalytic domain is the minimum peptide sequence which retains the catalytic property inherent in that amino acid sequence. Catalytic antibodies as used herein thus include any fragment or domain in which a binding region is involved in the catalysis. fw>wtaf»ønt» of the Invention The invention provides an antibody able to catalyze a chemical reaction of a polypeptide, methods for eliciting an antibody able to catalyze a chemical reaction of a polypeptide, and methods of using the antibody to cause a selected polypeptide to undergo a chemical reaction. The antibody is elicited to a ground state antigen which, depending on the immunogenicity of the antigen, iε a εelected peptide, peptide derivative or peptide fragment, or is formed by coupling a selected peptide, peptide derivative or peptide fragment to any useful immunological carrier known to the art. The invention alεo provides an antibody light chain able to catalyze a chemical reaction of a reactant including polypeptides and other substrates, methods for eliciting an antibody from which that light chain able to catalyze a chemical reaction may be derived, and methods for uεing the antibody to cause a selected reactant to undergo a chemical reaction. The antibody is elicited to a ground state antigen formed by coupling a selected reactant, reactant derivative or reactant fragment to any useful immunological carrier known to the art. The antibody thus elicited is then caused to separate into its component heavy and light chains, and the light chains are then isolated. The antibodies of the invention can be monoclonal or polyclonal. Monoclonal antibodies are prepared by isolating lymphocytes from animals identified as having antibodies to a particular antigen, producing a plurality of hybridomas from the isolated lymphocytes and then screening the monoclonal antibodies produced by the hybridomas to identify monoclonal antibodies which enhance the rate of chemical reaction of the substrate. The antibody-producing lymphocytes are hybridized with myeloma cells, such as, for example SP2/0 or NS1 cells, to produce hybridoma cells. These hybridoma cells are then plated in the wells of microtiter plates. The monoclonal antibodies produced by the hybridoma cells are screened under appropriate conditions to identify monoclonal antibodies which enhance the rate of the reaction under appropriate conditions. The identification is made by treating a standardized solution of the reactant/substrate with an aliquot withdrawn from a microtiter well and screening for the presence of the desired product, as described above. By comparison with standardized samples of the desired product or reactant/substrate, rates of reaction can be quantified. In this manner, wells containing hybridoma cells producing catalytic monoclonal antibodies can be identified. The selected hybridoma cells were then cultured to yield colonies. These colonies are further propagated in in vitro or in vivo systemε. In the latter caεe, mice such as εyngeneic BALB/C mice are inoculated intraperitoneally with the εelected hybridoma cells and produce tumors, generally within two or three weeks. These tumors were accompanied by the production of ascites fluid which contains the desired monoclonal antibodies. The monoclonal antibodies are then separately recovered from the ascites fluid by conventional methods such as ultrafiltration, ultracentrifugation, dialysis and immunoaffinity chromatography. Peripheral blood lymphocytes of an animal identified as having catalytic antibodies for a particular substrate can be stimulated to grow in culture and, therefore, can be immortalized using methodologies well known in the art. For example, the lymphocytes can be so stimulated using a virus, a chemical agent or a nucleic acid (e.g., an oncogene) . A particularly advantageous virus for immortalization is Epstein Barr virus (EBV) . Thus, catalytic antibodies can be produced by the transformed cells. The so transformed cells can then be cloned using known methods to provide a reliable source of large amounts of monoclonal antibodies having catalytic activity for a given substrate. One skilled in the art will appreciate that the genes, or fragments thereof, coding for the variable region of the antibody can be expressed in prokaryotic and eukaryotic cells using recombinant DNA methodologies well known in the art. In accordance with an embodiment of the invention, the separately recovered antibodies are contacted with a molecule (e.g., a substrate, antigen, etc.) under suitable conditions permitting the formation of a complex between the antibody and the molecule in order to achieve catalysis of a chemical reaction of the molecule. The skilled artisan will appreciate that the conditions εuitable for complex formation can vary depending on the particular molecule and antibody under consideration. Accordingly, the methods of this invention may be practiced under a variety of reaction conditions, in vivo and in vitro , as long as the antibodies are not prevented from complexing with the molecules or otherwise rendered inactive. More specifically, suitable conditions for complex formation encompass εolution phase and emulsion reaction systems including a variety of solvents and solvent systems, maintained at a pH value between about 6.0 and about 9.0, preferably between about 6.0 and about 8.5 and at a temperature from about 4"C to about 50°c, preferably from about 20 β C to about 45 β c. One of ordinary skill in the art will realize that the choice of solvent will depend on the type of reaction. For example, aqueous solvents are desirable for peptide bond cleavage while non-aqueous solvents can be used to achieve peptide bond formation. The ionic strength, = 1/2 Σ c i z i 2, where c is the concentration and z is the electronic charge of an ionic solute, should be maintained at a value below about 2.0 (ionic strength units), preferably between 0.1 and 1.5. The methods of the invention can be carried out at reduced or elevated pressure, but advantageously are practiced at ambient pressure. In addition to solution phase and emulsion reaction syεtems, suitable conditions also include the use of solid support materials to which the antibody is attached. Such solid support materials are well-known to those of ordinary skill in the art as are methods for attaching antibodies to them. A specific embodiment of the invention is directed to an antibody to vasoactive inteεtinal peptide (VIP) . VIP iε a 28 amino acid peptide originally isolated from the intestine but now recognized to be a neuropeptide widely distributed in the central and peripheral nervous systems. There is evidence that VIP is a neurotransmitter in itε own right. In addition, VIP may modulate neurotransmisεion by classical tranεmitterε and haε been implicated in regulation of blood pressure, bronchial tone, neuroendocrine activity and exocrine secretion. VIP appearε to be the major neurobronchodilator in humans and a diminished influence of VIP on the airwayε may permit a dominance of constrictor influences, and may underlie airway hyperactivity in asthma. VIP belongs to a family of εtructurally related peptides, other prominent members of which are peptide histidine isolucine (PHI) , growth hormone releasing factor (GRF) and secretin. Like the peptides themselves, there is evidence that the receptors for VIP, GRF, PHI and secretin are related. Receptors for VIP are found in lung, vascular smooth muscle, brain, pancreas, skin, intestine and other tissues. The amino acid sequence of VIP is aε follows: H S D A V F T D N Y T R L R K Q M A V K K W L N S I L M-CO-HH 2 . Monoclonal antibodies to VIP were prepared by methods well known to the art (19-25) . In one embodiment of this invention, a series of mice were immunized by an antigen comprising vasoactive inteεtinal peptide (VIP) coupled to keyhole limpet hemocyanin (KLH) . The sera of the mice was screened for the saturable (Y 10 - 125 I)VIP binding titer, and splenocytes from the mouse showing the greatest saturable (Y 10 - 125 I)VIP binding titer were used to prepare hybridomas. Hybridoma clones were grown as ascites in host mice and the resulting antibodies were purified from the ascites fluid. Antibodies C23.5 and c23.4 were purified and screened for VIP hydrolytic activity. Both antibodies hydrolyzed (Y 10 - 125 I)VTP, whereas irrelevant antibodies used as controls did not. An Fab fragment of C23.5 also demonstrated VIP hydrolytic activity. While VIP is the exemplified substrate and hapten, it will be understood that any peptide may be used as a εubstrate and hapten. It is well known that VIP is a natural polypeptide composed of the εame amino acids found in other peptides and proteins. Several naturally occurring neurotransmitters and hormones are closely related in primary structure to VIP. These include: secretin, glucagon, growth hormone releasing factor, and helodermin (26) . While VIP is the exemplified substrate and hapten, it will be understood that any peptide may be used aε a subεtrate and hapten. Many different bonds in VIP can be cleaved by the VIP autoantibodies disclosed in parent application USSN 342,081, filed April 4, 1989. (see also, Paul, S., et al. (27) . Thus antibody-mediated cleavage is not restricted to a unique peptide bond. The scissile peptide bonds in VIP (e.g., Gin-Met, Ala-Val, Met-Ala, Lys-Lys) (Paul et al. supra) are alεo present in other polypeptides. The catalytic antibodies cleave not only full- length VIP, but also a fragment of VIP [VIP(15-28)] (23). Thiε shows that the N-terminal region of VIP is not required for antibody catalytic activity. This is important because the possibility has previously been considered that the N-terminal residues of VIP may facilitate autocleavage of this peptide (28) . Since the recognition of VIP by antibodies occurs by the same pattern aε for other polypeptide antigenε, viz., by recognition of linear or conformational epitopes composed of diεcrete peptide subsequences found in the parent antigen (29) , it is likely that antibodies recognizing other polypeptide antigens will display catalytic activity, provided the conditions deεcribed below are met. The recognition sequence iε a more important determinant than the type of bond to be cleaved. Thiε conclusion is supported by studies showing that VIP(22-28) inhibits cleavage of VIP at residues 16-17 by antibodies (23) . Therefore, there iε nothing unique about the VIP cleavage point. Antibodies possess hypervariable antigen binding sites (30) . The hypervariability is brought about by V-D-J junctional rearrangement and somatic hypermutation, and 10 12 different antibodies can be syntheεized by B-lymphocyteε. Antibody molecules already fulfill the first criterion required of a catalyst, i.e., the ability to bind subεtrate. Catalysis by enzymes iε brought about by ppεitioning of specific amino acid residues (e.g., His, Ser, Asp, Cys) or cofactorβ (e.g., metals) in the substrate binding site (31) . Enzymes which cleave polypeptides, i.e. proteases, are inhibited by diisopropyl fluorophosphate (DFP) , which blocks the residueε having protease activity. Given that a large number of antibody binding sites are created by the immune system by natural mutagenesis, it is most probable that some antibodies directed against polypeptide antigens would contain protease activity in their binding εiteε. The presence of the protease activity in the binding site can be independent of the type of antigen. The only requirements that must be met are specific binding and presence of suitably positioned catalytic amino acidε in the antibody active εite. Therefore, the skilled artisan will appreciate that the following description of anti-VlP catalytic antibodies and catalytic antibody light chains is by way of example only, and is not intended to limit the scope of the invention. EXAMPLE 1 MURINE MONOCLONAL CATALYTIC ANTIBODIES Conjugates of VIP and keyhole limpet hemocyanin (VIP-KLH) were prepared via coupling to primary NH 2 groups on VIP and to -SH groups on thiolated KLH using the crosslinking reagent gamma- maleimidobutyryloxyεuccinimide (GMBS) . KLH (17 mg) was thiolated with iminothiolane (33) the thiolated KHL was fractionated on a gel filtration column (Biorad Econo-Pak 10 DG) and conjugated with 3 mg synthetic VIP that had previously been treated for 2 h with GMBS. The molar stoichiometry of the VIP:KLH conjunction was 360, eεtimated on the baεiε of depletion of thiol groups reactive with Ellman'ε reagent. Five mice were immunized with the reεulting VIP-KLH. Primary immunization (in Freundε complete adjuvant) followed by two booster immunizations (in incomplete Freunds adjuvant) waε at 2- week intervals using 10-50 μg VIP equivalents of the antigen conjugate, administered intraperitoneally. The final injection was an intrasplenic one (17 μg VIP equivalents) . Splenocytes from the mouse with the greatest saturable (Y 10 - 125 I)VIP binding titer (10% binding, 1:200 serum dilution; assayed according to Paul et al. (34) were fused with NS-1 myeloma cells by the PEG method (35). Cells from three culture wells (c23.1, C23.4 and C23.5) positive for VIP binding antibody were cloned twice by limiting dilution and then grown as ascites in pristane-treated mice. Antibodies c23.5 and c23.4 (both identified to be IgG 2a , kappa by ELISA) were purified from tissue culture fluid or ascites fluid by ammonium sulfate precipitation and Protein G-Sepharose chromatography aε described by Paul et al. (36) . Both antibodies hydrolyzed (Y 10 - 125 I)VIP and the hydrolysis products were asεayed by εeparation of intact and degraded peptideε by trichloroacetic acid (TCA) precipitation. See Paul et al. (36) for the aεεay method. The detection of hydrolytic activity waε a function of the concentration of the antibody. Surpriεingly, no hydrolytic activity waε observed at high antibody concentrations (> 10 nM) . The properties of one of the antibodieε (c23.5) were analyzed further. The strategies utilized to characterize the monoclonal antibodies were similar to those uεed for human antibodieε (19-25) . The hydrolytic activity of thiε antibody waε confirmed by reverεe phase HPLC of the reaction mixture. Treatment of (Y 10 - 125 I)VIP with this antibody resulted in the appearance of two early eluting peaks of radioactivity, corresponding to VIP fragments (retention times 46 and 50 min. compared to 66 min. of intact (Y 10 ~ 125 I)VIP. Other monoclonal antibodieε to VIP [c23.l (lgG 2 , kappa) and c.5162 (lgG 2 , kappa); obtained from Dr. J. Porter, University of Texas Health Science Center, Dallas) ] were shown to be without VIP hydrolytic activity. Irrelevant monoclonal antibodies purified from hybridoma tissue culture fluids (directed against insulin-degrading enzyme, LH-receptors, CD8 or whole T- lymphocytes; obtained from Monoclonal Antibody Core Facility, University of Nebraska) were examined for (Y 10 - 125 I)VIP cleaving activity, and were observed to be without such activity aε were irrelevant antibodieε purified from commercially available ascites fluid (Sigma Chemical Company, St. Louis, MO) . Fab was prepared by digestion of protein G- purified IgG with immobilized papain (Pierce; 16 mg protein per ml gel in 20 mM sodium phosphate, 10 mM EDTA. ph 7 for 2 h) and chromatography on an anion-exchange column (Mono Q; Pharmacia, Piscataway, New Jersey); 0-1 NaCl in 20 mM Tris-HCl, pH 8, in 45 min). Fab, recovered in the unbound fraction, diεplayed a mass of 50 kD estimated by SDS-polyacrylamide gel electrophoresis (Fig. 1) . With increasing Fab concentration, progressively increasing hydrolysiε of VIP waε observed (Fig. 2). Evaluation using TCA precipitation to separate intact VIP from fragments indicates that hydrolysis of VIP by antibody c23.5 follows Michaelis-Menten kinetics (K,,, 2 nM, kcat 8 x 10 "5 /min) (Fig. 3) . Binding studies under conditions that did not permit VIP hydrolysiε (high ionic strength) yielded a K d estimate of 3.4 nM (37). The specificity of the observed antibody hydrolytic activity was confirmed by the failure of the antibody to hydrolyze unrelated tripeptide methylcoumarinamideε (Boc-L-R-R-MCA; and, Z-R-R-MCA) , assayed as the fluorescence of the leaving group [methylcoumarinamide (MCA) ] (Excitation wavelength, 370 mm; Emission wavelength, 460 nm) . In this experiment, 100 nM c23.5 antibody was incubated with 10 μg/ l substrate in microtiter plated (reaction voluae 50 μl) for 14 h in 100 nM Tris-HCl, 50 mM glycine, 0.025% Tween- 20 pH 8 (Buffer A) . Fluorescence values with the antibody were always < 1 unit. In comparison, bovine pancreatic trypsin yielded values of 263, 194 and 179 units using Boc-L-R-R-MCA, P-F-R-MCA and Z-R-R-MCA, respectively. EXAMPLE 2 MONOCLONAL L-CHAIN MEDIATED VIP HYDROLYSIS C23.4 antibody purified by protein G-Sepharose chromatography (Ex. l) (0.76 mg) waε reduced with 2- mercaptoethanol 0.2M for 3 h and then alkylated with iodoacetamide (0.3M) for 15 min, while the pH was maintained at 8 by addition of 1 M Tris base. The reduced and alkylated antibody was then treated with 6 M guanidinium chloride for 2 h, followed by fractionation by gel filtration in 6 M guanidinium chloride on two Superose-12 columnε attached in tandem at a flow rate of 0.4 ml/min (Pharmacia, Piεcataway, New Jerεey) . The column fractionε were dialyzed extenεively against Buffer A (see Example 1 for composition) and then fractionated by filtration (Superdex) . This purification protocol permitted preparation of a 25 kD L-chain fraction (retention time 60 min) apparently free of H-chain contamination, judged by SDS-polyacrylamide gel electrophoresis. Earlier eluting fractions from the column appeared to be enriched in H-chains, but also contained significant amounts of L-chains (26 Kd) and 90 kD constituents which may be H-L dimers. The pure L- chain fraction exhibited VIP binding (9xl0 3 CPM/μg protein) and hydrolyεiε (Fig. 4) when aεsayed according to Paul et al. (19). In a second experiment, c23.5 antibody was treated with 143 mM 2-mercaptoethanol, and 0.4% SDS in a Tris-HCL buffer, pH 6.8 and electrophoresed on a 7.5% SDS-polyacrylamide gel uεing a Schleicher and Schuellminielectrophoresis syεtem (22-30 mA/gel) . The bands corresponding to the H-chain (60 kD) and L-chain (25 kD) were exciεed, electroeluted (Biorad Model 422 eluter; 8-10 mA/elution tube) , the eluate waε electrodialyzed against buffer that did not contain SDS and subjected to Extractigel chromatography (Pierce, Rockford, II) in 50 mM Tris-HCl, pH 9, to remove residual SDS. The sampleε were then brought into Buffer A by 2- cycleε of ultrafiltration on Centriprep-10 ultrafilterε (Amicon) . The L-chain preparation displayed dose- dependent hydrolyεis (Fig. 5) . EXAMPLE 3 CLONING AND SEQUENCING OF C23.5 L-CHAIN AND H-CHAIN CDNA Total RNA waε prepared from 10 8 cells (c23.5) by the one-step guanidine-isothiocyanate method. cDNA was made from the C23.5 RNA using reverse transcriptase. Forward primers used for the reverse transcriptase reaction and forward and back primers used to amplify the cDNA for V L , L-chain (V L + C L ) , V H and Fd-chain (V H + C H L) are shown in Table l. The primers were designed based on consenεuε sequences present in L-chain (kappa) and heavy chains (lgG 2a ) (38-42) . BamHI or Xbal reεtriction sites were incorporated into the backward primers and EcoRI siteε into the forward primers to facilitate cloning. The cDNA was then amplified by polymerase chain reaction using Taq DNA polymerase (30 cycles; 1 min at 95 C, 2 min at 55 C and 3 min at 72 C for each cycle) . The amplified cDNA exhibited the expected sizeε determined by agarose gel electrophoresiε. The L-chain variable region cDNA waε then cloned into a pBlueεcriptllKS "1" vector (Stratagene, La Jolla, California) via the EcoRI and Xbal restriction sites. The ligated vector was introduced into Escherichia coli and transformed colonies (white) were selected in LB agar supplemented with X-gal and ampicillin. Plasmid DNA prepared from these colonies and digested with EcoRI exhibited a larger size (3.35 kb) than the pBluescript (3.0 kb) , corresponding to the length of the DNA insert. The DNA insert was sequenced by the dideoxy chain termination method using 35 S-ATP precursor and T3 and T7 sequencing primers. Ten colonies containing inserts were sequenced and the deduced sequence (Table 2) in each case waε identical. The H-chain variable region cDNA waε cloned and sequenced in a similar manner. TABLE 1 PCR PRIMERS FOR pBluescript II L-Chain Primers (SEQ ID NO:l) BcoRI L-v 5' 5' GGAATTC GAC ATT GTG CTG ACC CAR TCT CC 3' (SEQ ID NO:2) BamHI L-v 3' 5' cgggatcc cag ctt ggt ccc ccc ice gaa eg 3' (SEQ ID NO: 3 ) Xba L-c 3 ' 5 " gctctaga etc att cct gtt gaa get ctt gac 3 * H-Chain Primers (SEQ ID NO: ) EcoRI H-v 5' 5' GGAATTC GAG GTI CAG CTT CAG SAG TC GG 3* (SEQ ID NO:5) BamHI H-v 3' 5' cgggatcc ggt gas crk ggt ice tkk gcc cca g 3' (SEQ ID NO:6) Xbal H-c 3 • 5' gctctaga tgt trt ggg cac tct ggg etc 3' Synthetic oligonucleotide primers used to amplify C23.5 L-chains (SEQ ID N0:1 and SEQ ID NO:3), V L (SEQ ID NO:l and SEQ ID NO:2), Fd (SEQ ID NO:4 and SEQ ID NO:6), and V H (SEQ ID NO:4 and SEQ ID NO:5). Forward primerε (5') are εhown in upper case and backward primers (3') in lower caεe. R iε G or A; K is G or T; S iε G or C; W is A or T and I is inosine. TABLE 2 Sequence of c-23:5 VL fSEO ID NO:7) 1 21 41 GAC ATT GTG CTG ACC CAG TCT CCT GCC TCC CAG TCT GCA TCT CTG asp lie val leu thr gin βer pro ala ser gin ser ala aer leu 61 81 GGA GAA AGT GTC ACC ATC ACA TGC CTG GCA AGT CAG ACC ATT GGT gly glu aer val thr ile thr cys leu ala aer σln thr ile olv 101 121 ACA TGG TTA CCA TGG TAT CAG CAG AAA CTA GGG AAA TCT CCT CAG thr trp leu pro trp tyr gin gin lys leu gly lye aer pro gin 141 161 CTC CTG ATA TAT GCT GCA ACC AGC TTG GCA GAT GGG GTC CCA TCA leu leu ile tyr ala ala thr aer leu ala asp gly val pro βer 181 201 221 AGG TTC AGT GGT AGT GGA TCT GCC ACA AAA TTT TCT TTC AAG ATC arg phe aer gly aer gly aer ala thr lye phe aer phe lya ile 241 261 AGC AGC CTA CAG GCT GAA GAT TTT GTA AGT TAT AAC TGT CAA CAT aer aer leu gin ala glu asp phe val aer tyr asn cys oln his 281 301 CTT TAC AGT ACT CCG CTC ACG TTC GGC GGG GGG ACC AAG CTG leu tyr aer thr pro leu thr phe gly gly gly thr lye leu Sequence of c-23:5 VH (SEQ ID NO:8) 1 21 41 GAG GTG CAG CTT CAG GAG TCT GGG GGA GGC TTA GTG AAG CCT GGA glu val gin leu gin glu aer gly gly gly leu val lya pro gly 61 81 GGG TCC CTG AAA CTC TCC TGT GCA GCC TCT GGA TTC ACT TTC AGT gly aer leu lya leu aer eye ala ala aer gly phe thr phe aer 101 121 ATC TAT GGC ATG TCT TGG TTT CGC CAG ACT CCG GAG AAG AGG CTG ile tyr olv met aer trp phe arg gin thr pro glu lya arg leu 141 161 GAG TGG GTC GCA ACA ATT AGT GGT GGT GAT ACT TAC ACC TAC TAT glu trp val ala thr ile ser olv olv asp thr tyr thr tyr tyr 181 201 221 CCA GAC AGT GTG AAG GGG CGA TTC ACC ATC TCC AGA GAC AAT GCC pro asp aer val lvs olv arg phe thr ile ser arg asp aβn ala 241 261 AAG AAC AAC CTG TTC CTG CAA ATG AGC AGT CTG AGG TCT GAG GAC lys asn aβn leu phe leu gin met aer ser leu arg aer glu asp 281 301 ACG GCC TTG TAT TTC TGT GGA AGA GGG ATT GCT TAC TGG GGC CAA thr ala leu tyr phe cys gly arg αlv ile ala tyr trp gly gin 321 GGG ACT CTG GTC ACT GTC TCT GCA gly thr leu val thr val βer ala Underlining indicates complementarity determining regions (CDR' s) of the antibody chain . EXAMPLE 4 EVIDENCE FOR BUBUNIT REARRANGEMENT Antibodies are composed of four subunits held by disulfide bonds. Reduction and reformation of disulfide bonds could conceivably result in formation of a variety of antibody oligomers (e.g., H-H, L-L r H-L) . Analogous disulfide bond exchange reactions are shown to occur in other oligomeric proteins (32) . The importance of such rearrangements for antibody binding activity is probably minimal since dissociated antibody subunits display low binding activity, and variant molecules composed of incorrectly paired subunitε are alεo likely to poεεeεε low binding activity. In regard to antibody catalyεiε, however, it haε been observed that purified L- chains hydrolyze VIP faster than undissociated antibody preparations. Formation of rearranged antibody oligomers may well be accompanied by increased hydrolytic activity, if homologous H-chains are better inhibitors of L-chain catalytic activity than heterologous H-chains. Evidence that subunit rearrangement in VIP antibodies may form new catalytic species is as follows. Anion exchange chromatography (Mono Q column, Pharmacia) (0-1 M NaCl in 40 min; 1 min fractions were collected) of 0.55 mg c23.5 antibody that had previously been purified by affinity chromatography on immobilized Protein G produced a εharply eluting protein peak, correεponding to fractionε 32 and 33 (Fig. 6) . Following dialysis to remove NaCl, the effluent fractions were assayed for VIP hydrolysis. Fractions corresponding to the main protein peak (fractions 32-33) displayed VIP hydrolytic activity. In addition, VIP hydrolytic activity waε observed in fractions 34-38, located at the trailing edge of the protein peak was evident. To determine whether the hydrolytic activity was due to antibodies, the fractions (0.03 ml) were treated with 0.22 ml gel of anti-mouse IgG conjugated to Sepharose (Organon Teknika, Durham, North Carolina) or an equivalent amount of control anti-rabbit IgG conjugated to Sepharose (Jackson Immuno Research, West Grove, Pennsylvania) for 13 h in a final volume of 5 ml Buffer A (see Ex. 1), and the supernatantε (0.05 ml) of the gelε were aεεayed for VIP hydrolyεiε. The hydrolytic activity in all fractionε was nearly completely adsorbed by immobilized anti-mouse IgG, but not by immobilized anti- rabbit IgG (Fig. 7) . SDS-polyacrylamide gel electrophoresiε under reducing conditions and immunoblotting using a specific anti-mouεe IgG antibody εhowed that all fractions were compoεed of 60 kD H- and 25 kD L-chainε. The late eluting fractions also contained a 90 kD band that was stainable with the anti- mouse IgG and represents an H-L dimer. Electrophoresis under non-reducing conditions revealed that the main protein peak was composed of intact 150 kD antibodies while the trailing peak was composed of 90-120 kD bands, representing antibody oligomers (Fig. 8) . EXAMPLE 5 CHARACTERIZATION OF ANTI-VIP ANTIBODY λ. Kinetic Data and Binding Effects VIP has been previously obεerved to undergo cleavage by human autoantibodies at several bonds located between residues 14 and 22 (36) . A εynthetic peptide mimic [ABZ-RKQMAVKKY(N02)D] Of VIP(14-22) (RKQMAVKKY (SEQ ID No:9); designated pep2) was tested as substrate for the monoclonal antibody. The anthraniloyl group at the N-terminus of thiε εynthetic peptide in combination with the Y(N0 2 ) reεidue cloεe to the c-terminuε functionε as an intramolecularly quenched fluorescence reporter system for hydrolysiε at peptide bonds located between the fluorophore (ABz) and quencher [Y(N02)]. Synthesiε of thiε peptide waε according to Meldal, M. et al. (43). Treatment of pep2 with the increasing concentrations of C23.5 antibody monoclonal VIP antibody resulted in progressive hydrolysiε, determined by an increase in fluorescence emission at 420 nm (excitation wavelength, 370 nm) (Fig. 9) . Initial rate data obtained by varying the substrate concentrations were consistent with Michaeliε- Menten kinetics. Reversed-phase HPLC analysiε on a C-18 column using trifluoroacetic acid in acetonitrile for elution, showed the appearance of two new peptide peaks following treatment of pepl (20 μM) with C23.5 antibody (20 μM) for 100 h in MOPS buffer (Fig. 10) . These were sequenced on an automated Applied Biosystems peptide liquide phase sequenator equipped with online PTH-amino acid detection. The two peptides were identified to be RKQMAVK and KY(N02)D, indicating that the scisεile bond is a K-K bond, corresponding to K 20 -K 21 in intact VIP. REFERENCES 1. Pauling, L. Nature 161:707 (1948) 2. Kohen, F. Kim, J. B. , Linder, H.R. , Eshkar, Z., Green, A.B. Antibody enhanced hydrolyεiε of βteroid eεters, Biochemica et Biophvsica Acta 629, 328-337 (1980) . 3. Pollack, S.J., Jacobs, J.W. , Schultz, P.J., Science 234, 1570 (1986). 4. Tramontano, A., Amman, A.A. , Lerner, R.A. , J. Am. Chem et Soc. 110, 2282 (1988). 5. Janda, K.D., Schloeder, D. , Benkovic, S.J., Lerner, R.A., Science 241, 1188 (1988). 6. Durfor, C.N., Bolin, R.J. , Sugasawara, R.J., Massey, R.J., Jacobs, J.W. , Schultz, P.J. , J. Am. Chem. Soc. 110, 8713 (1988) . 7. Jackson, D.Y., Jacobs, J.W. , Sugasawara, R. , Reich, S.H. , Bartlett, P.A. , Schultz, P.G., J. Am. Chem. Soc. 110, 4841 (1988) . 8. Hilvert, D., Carpenter, S.H., Nared, K.D., Auditor, N.T., Proc. Natl. Acad. Sci. USA 85, 4953 (1988). 9. Shokat, K. Leu ann, C.H. , Sugasawara, R. , Schultz, P.G., Anqew. Chem. Int. Ed. Engl. 27, 1172 (1988). 10. Titmas et al., R.C., Peptide Analogs and Their Use As Hapten to Elect Antibodies PCT US89/01951 filed 5/14/89. 11. Suckling, C.J. , Tedford, CM., Proctor, G.R. , Khalaf, A.I., Bence, L.M. and Stimson, W.H. 1991 CATALYTIC ANTIBODIES. Wiley, Chichester (Ciba Foundation Symposium 159) p.201-210. 12. Iverson, B.L. and Lerner, R.A. , Science 243, 1184 (1989) . 13. Roholt, O. , Onoue, K. , and Pressman, D. Biochemistry Sλ, 173, (1964). 14. Ward, E.S., Gussow, D. Griffiths, A.D., Jones, P.T. and Winter, G. Nature. 341, 544-546 (1989). 15. Edelman, G.M. , Olins, D.E. , Gaily, J.A. and Zinder, N.D. Proc. Natl. Acad. Sci.. 50, 753 - 761 (1963). 16. Franek, F. and Nezlin, R.S. Folia Microbiol.. 8, 128-130 (1963) . 17. Franek, F. and Nezlin, R.S. Biokhimiva. 28, 193 (1963) . 18. Masεey, R.J. et al. U.S. Patent No. 4,888,281. 19. Paul, S., Voile, D.J., Beach, CM., Johnson, D.R. , Powell, M.J. and Masεey, R. . : Catalytic hydrolysis of vasoactive intestinal peptide by human autoantibody. Science. 244, 1158-1162 (1989). 20. Paul, S., Mei, S., Mody, B. , Eklund, S.H. , Beach, CM., Masεey, R.J. and Hamel, F. Cleavage of VIP at multiple sites by autoantibodies. J.Biol.Chero. In press (1991) . 21. Mei, S. r Mody, B. , Eklund, S.H. and Paul, S.: VIP hydrolysis by antibody light chains. J.Biol.Chem. In preεs (1991) . 22. Paul, S., Voile, D.J. and Mei, S. : Affinity chromatography of catalytic autoantibody to vasoactive intestinal peptide. J.Immunol.. 145, 1196-1199 (1990). 23. Paul, S., Voile, D.J. , Powell, M.J. and Massey, R.J. : Site specificity of a catalytic vasoactive inteεtinal peptide antibody: An inhibitory VIP subsequence distant from the sciεεile peptide bond. J.Biol.Chem.. 365, 11910-11913 (1990). 24. Paul, S.: A new effector mechanism for antibodies: Catalytic cleavage of peptide bonds. Cold Spring Harb. Symp. Quant. Biol.. 54, 283-286 (1989). 25. Paul, S., Johnson, D.J. and Massey, R.J. Binding epitopes and multiple hydrolytic siteε recognized by catalytic antibodies. In CXBA Foundation Symp. 159 on Catalytic Antibodieε, In Presε, (1991) . 26. Mutt, V. Vasoactive Intestinal Peptide and related peptides: Isolation and chemistry. Annals of the New York Academy of Sciences 527, 1-19 (1988). 27. Paul, S. , Mei, S. , Mody, B. , Eklund, S.H., Beach, CM., Masεey, R.J. and Hamel, F. Cleavage of VIP at multiple εiteε by autoantibodies. J.Biol.Chem. In press 1991 (August issue) . 28. Nishi, N. , Tsutuεu i, A., Moriεhige, M. , Kiyama, S. , Fuji, N., Takeyama, M. Yaji a, H. Apparent autolysiε of the N-terminal tetrapeptide of VIP. Chem. Pharm. Bull. 31, 1067-1072 (1983). 29. Davies, D.R. , Padlan, E.A. and Sheriff, S. : Antibody-antigen complexes. Ann. Rev. Biochem.. 59, 439- 474 (1990) . 30. French, D.L., R. Laskow, and M.D. Scharff. The role of somatic hypermutation in the generation of antibody diversity. Science 244, 1152 (1989). 31. Barrett, A.J.: An introduction to proteinases. In: Proteinaεe Inhibitors. Eds.: A.J. Barrett and G. Salvesen, Elsevier, pp. 3-22 (1986) . 32. Creighton, T.E. Disulfide bonds between cystein reεidueε. In Protein Structure: A Practical Approach (Ed. T.E. Creighton) IRL Press, Oxford, U.K., pp. 155- 167. 33. Jue et al., Biochemistry 17, 5399-5406, (1978). 34. Paul, S. , Said, S.I., Thompson, A. Voile, DJ Agrawol, D.K. , Foda, H, and De la Rocha, S. Characterization of autoantibodies to VIP in asthma. J. Neuroimmunol.. 23, 133-142 (1989). 35.. Brown, G. and Ling, N.R. Murine Monoclonal Antibodies. In Antibodies : A Practical Approach (Ed: D.Cathy) IRL Press, Oxford, U.K. Vol. 1, page 81-104 (1988) . 36. Paul et al. J. of Biol. Chem. 266, 16128-16134 (1991) . 37. Baldwin E. and Schuyltz, P.G., Generation of a catalytic antibody by site-directed mutagenesis. Science. 245, 1104-1107 (1989). 38. Kabat, E.A., Wu, T.T., Reid-Miller, M. , Perry, H.M. and Gottesman, K.S.: Sequences of proteins of immunological interest (4th ed.), U.S. Department of Health and Human Services. 39. Orlandi, R. , Gussow, D.H. Jones, P.Y. and Winter, G.: Cloning immunoglobulin variable domainε for expreεεion by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA. 86, 3833-3837, (1989). 40. Sastry, L. , Alting-Mees, M. , Huse, W.D., Short, J.M. , Sorge, J.A. , Hay, B.N. , Janda, K.D. Benkovic, S.J. and Lerner, R.A. : Cloning of the immunological repertoire in Eεcherichia coli for generation of monoclonal catalytic antibodies: Construction of a heavy chain variable region-specific cDNA library. Proc. Natl. Acad. Sci. , USA. 86, 5728-5732, (1989). 41. Huse, W.D., Sastry, L. , Iverson, S.A. , Kang, A.S., Alting-Mees, M. , Burton, D.R. , Benkovic, S.J. and Lerner, R.A. : Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda. Science. 246, 1275-1281, (1989) . 42. Larrick, J.W. , Danielsεon, L. , Brenner, CA., Abrahamεon, M. , Fry, K.E. and Borrebaeck, C.A.K. : Rapid cloning of rearranged immunoglobulin genes from human hybridoma cells using mixed primers and the polymerase chain reaction. Biochem. Biophys. Res. Commun.. 160, 1250-1256, (1989). 43. Meldal, M. et al., Bredda , K., Analytical Biochemistry. 195, 141-147, (1991).

Claims: WHAT IS CLAIMED IS: 1. A method of catalyzing a chemical reaction of a polypeptide comprising contacting said polypeptide with at least one catalytic monoclonal antibody produced by a process comprising the steps of: a. generating a plurality of monoclonal antibodies to an antigen selected from the group consisting of: i. said polypeptide, ii. a derivative of said polypeptide, and iii. a fragment of said polypeptide; and b. screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes said chemical reaction. 2. A method as recited in claim 1 wherein said polypeptide is vasoactive intestinal peptide and said chemical reaction is hydrolysiε of said polypeptide at at least one peptide bond. 3. A method of catalyzing a chemical reaction of a polypeptide comprising contacting said polypeptide with at least one catalytic monoclonal antibody produced by a procesε compriεing the steps of: a. immunizing an animal with an antigen selected from the group consisting of i. said polypeptide, ii. a derivative of said polypeptide, and iii. a fragment of said polypeptide; and thereby generating antibody-producing lymphocytes in said animal; b. removing said antibody-producing lymphocytes from said animal; c. fusing said antibody-producing lymphocytes with myeloma cells and thereby producing a plurality of hybridoma cells each producing monoclonal antibodies; and d. screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction. 4. A process for making a catalytic antibody able to catalyze a chemical reaction of a polypeptide comprising the steps of: a. eliciting an immune responεe to εaid polypeptide; and b. screening immune cells or antibodieε resulting from said immune response to identify immune cells or antibodies having the property of catalyzing the chemical reaction of said polypeptide. 5. A procesε aε recited in claim 3 wherein said polypeptide is vasoactive intestinal peptide and said chemical reaction is hydrolysis of said polypeptide at at least one peptide bond. 6. A procesε as recited in claim 4 wherein said polypeptide is vasoactive intestinal peptide and said chemical reaction is hydrolysis of said polypeptide at at least one peptide bond. 7. A process for making a catalytic antibody able to catalyze a chemical reaction of a polypeptide comprising the stepε of: a. generating a plurality of monoclonal antibodies to an antigen selected from the group of consiεting of: i. said polypeptide, ii. a derivative of said polypeptide, and iii. a fragment of said polypeptide; and b. screening said plurality of monoclonal antibodies to identify a monoclonal antibody having the property of catalyzing the chemical reaction of said polypeptide. 8. A catalytic antibody able to catalyze a chemical reaction of a polypeptide produced by a process comprising the steps of: a. eliciting an immune response to the polypeptide; and b. screening immune cells or antibodies resulting from said immune responεe to identify immune cells or antibodies having the property of catalyzing the chemical reaction of said polypeptide. 9. A catalytic antibody able to catalyze a chemical reaction of a polypeptide produced by a proceεs comprising the stepε of: a. generating a plurality of monoclonal antibodieε to an antigen selected from the group of consisting of: i. said polypeptide, ii. a derivative of said polypeptide, and iii. a fragment of said polypeptide; and b. screening εaid plurality of monoclonal antibodieε to identify a monoclonal antibody having the property of catalyzing the chemical reaction. 10. An antibody as recited in claim 9 wherein εaid polypeptide iε vasoactive intestinal peptide and said chemical reaction iε hydrolysis of said polypeptide at at least one peptide bond. 11. A method of catalyzing a chemical reaction of a reactant comprising contacting said reactant with a catalytic antibody light chain produced by a process comprising the εteps of: a. generating a plurality of monoclonal antibodieε to an antigen selected from the group consisting of: i. said reactant, ii. a derivative of εaid reactant, and iii. a fragment of εaid reactant; b. εcreening εaid plurality of monoclonal antibodieε to identify a monoclonal antibody which catalyzeε εaid chemical reaction; c. separating said catalytic monoclonal antibody into light chain and other componentε; and d. isolating said light chain component. 12. A method as recited in claim 11 wherein step c comprises the further steps of cleaving said catalytic monoclonal antibody to form an Fab fragment and reducing and then alkylating said Fab fragment under denaturing conditions, and wherein step d comprises fractionation by filtration. 13. A method of catalyzing a chemical reaction of a reactant comprising contacting εaid reactant with a catalytic antibody light chain produced by a proceεε compriεing the stepε of: a. immunizing an animal with an antigen selected from the group consisting of i. said reactant, ii. a derivative of said reactant, and iii. a fragment of εaid reactant; and thereby generating antibody-producing lymphocytes in said animal; b. removing said antibody-producing lymphocytes from said animal; c. fusing said antibody-producing lymphocytes with myeloma cells and thereby producing a plurality of hybridoma cells each producing monoclonal antibodies; d. screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction; and e. obtaining a catalytic antibody light chain of said catalytic monoclonal antibody. 14. The method as recited in claim 13 further comprising producing a catalytic antibody light chain by the stepε of: a. inεerting into a cell at leaεt one nucleic acid sequence coding for a variable region of said catalytic antibody light chain; b. replicating εaid cell; and c. expressing said nucleic acid sequence to produce a product having the desired catalytic activity. 15. A procesε for making a catalytic antibody light chain able to catalyze a chemical reaction of a reactant compriεing the steps of: a. generating a plurality of monoclonal antibodies to an antigen selected from the group consisting of: i. said reactant, ii. a derivative of said reactant, and iii. a fragment of said reactant; b. screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes said chemical reaction; c. εeparating said catalytic monoclonal antibody into light chain and other components; and d. isolating said light chain component. 16. The process aε recited in claim 15 wherein εtep c comprises the further stepε of cleaving said catalytic monoclonal antibody to form an Fab fragment and reducing and then alkylating said Fab fragment under denaturing conditionε, and wherein εtep d compriεes fractionation by filtration. 17. A procesε for making a catalytic antibody light chain able to catalyze a chemical reaction of a reactant compriεing the εteps of: a. immunizing an animal with an antigen εelected from the group consisting of i. εaid reactant, ii. a derivative of εaid reactant, and iii. a fragment of said reactant, thereby generating antibody-producing lymphocytes in εaid animal; b. removing εaid antibody-producing lymphocytes from said animal; c. fusing said antibody-producing lymphocytes with myeloma cells and thereby producing a plurality of hybridoma cells each producing monoclonal antibodies; d. screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction; and e. obtaining a catalytic antibody light chain of said catalytic monoclonal antibody. 18. A catalytic antibody light chain able to catalyze a chemical reaction of a reactant produced by a process compriεing the εtepε of: a. generating a plurality of monoclonal antibodies to an antigen selected from the group consisting of: i. said reactant, ii. a derivative of said reactant, and iii. a fragment of εaid reactant; b. screening said plurality of monoclonal antibodieε to identify a monoclonal antibody which catalyzes said chemical reaction; c. separating said catalytic monoclonal antibody into light chain and other components; and d. isolating said light chain component. 19. A catalytic antibody light chain as recited in claim 18 wherein step c compriseε the further steps of cleaving said catalytic monoclonal antibody to form an Fab fragment and reducing and then alkylating said Fab fragment under denaturing conditions, and wherein step d comprises fractionation by filtration. 20. A catalytic antibody light chain able to catalyze a chemical reaction of a reactant produced by a process comprising the steps of: a. immunizing an animal with an antigen selected from the group consisting of i. said reactant, ii. a derivative of said reactant, and iii. a fragment of εaid reactant; and thereby generating antibody-producing lymphocytes in said animal; b. removing said antibody-producing lymphocytes from said animal; c. fusing said antibody-producing lymphocytes with myeloma cells and thereby producing a plurality of hybridoma cells each producing monoclonal antibodies; d. screening said plurality of monoclonal antibodies to identify a monoclonal antibody which catalyzes the reaction; and e. obtaining a catalytic antibody light chain of said catalytic monoclonal antibody.

Inventors: PAUL SUDHIR, PAUL, SUDHIR
Applicants: IGEN INC, IGEN, INC.
Application year: 1992
Main classification: C12P21
IPC codes: C12N9, C12N15, C12P21