ELEMENTS AND METHODS EMPLOYING POLYMERIC SURFACE AMPLIFICATION AGENTS

Abstract: This invention describes a method for the production of a secondary or amplification reagent for use in assays based on thin film detection systems. The characteristics of particles that provide optimal performance in both visually interpreted and instrumentally analyzed detection systems are described. Amplification in these thin film systems may be achieved by use of materials which are capable of increasing thickness or optical density of the thin film generated in the reaction of receptive material and analyte. These assay systems are useful in the detection of reactions between a variety of interacting species.

Description: ELEMENTS AND METHODS EMPLOYING POLYMERIC SURFACE AMPLIFICATION AGENTS FIELD OF THE INVENTION Thin film detection methods which provide direct determination of interacting species have long been held to offer significant advantages relative to radioactive; or enzymic whether fluorescent, luminescent, colorimetric, or other tag-dependent detection schemes. These tag dependent detection systems require extensive optimization of a large number of parameters. These parameters include, for enzymatic systems: enzyme specific activity, level of incorporation of enzyme on secondary receptive material, amount of secondary reagent in test sample, and level of substrate(s) required. Thin film systems have suffered from a lower applicability in the detection of small molecules. Such analytes fail to produce sufficient thickness or optical density for visual or instrumented detection. Thin film detection systems perform optimally when the integrity of the film is maintained. Any method designed for amplification in such systems should provide an increase in thickness or density and maintain the film integrity as well as meet any limitations of the detection systems, and should be of the simplest possible construction. BACKGROUND OF THE INVENTION Description of the Prior Art Reports of amplification in thin film detection systems in the prior art are limited. One amplification method describes the use of small silica particles coated with concanavalin A or an anti-lgG antibody for use in an ellipsometric assay (C. Fredrik Mandenires and K. Mosbach, Anal. Biochem. , 170, 68-72, 1988). Silica particles provide a refractive index which is sufficiently close to the biological layers in the described system that the added layer serves to increase the apparent thickness of the biological layers in a concentration dependent manner. These particles being rigid in nature do not, however, maintain the integrity of the films. U.S. Patent 4,647,544 describes an immunoassay detection system which relies on Bragg scattering. The Bragg scattering is produced by use of a grooved solid substrate or by application of the antibody in patterns. Also described is a method for increased scattering by introduction of a secondary reagent composed of an antibody and a chemically inert carrier. The preferred embodiment utilizes a metal such as gold at 5-100 nm. Metals offer significant advantages in the scattering method. Additional secondary carriers proposed were cells and highly cross-linked, rigid polystyrene latex of 20-500 nm. The requirement for this system is that the carrier provide discontinuous, discrete, point sources for scattering. U.S. Patent 4,480,042 describes an agglutination immunoassay which relies on the attachment of antibody to high refractive index particles. The particles are constructed of a high refractive index shell or core latex polymer with a refractive index of 1.54 or greater. The outer shell provides a material suitable for the covalent attachment of the biomolecule. In this case, specifically a 0.03 to 0.1 micron particle is utilized. Again, for assessment of an agglutination reaction, the particles should provide discrete point sources for scattering. U.S. Patent 4,181,636 describes a similar agglutination immunoassay but does not require the high refractive index. The present invention describes the use of a styrene- butadiene copolymer latex with a specific gravity near water and which contains surface -C00H groups for covalent attachment of antibodies utilizing a water soluble carbodiimide. Again, the requirement for such latex particles is that they maintain their particulate character. A wide variety of styrene-butadiene copolymers have found utility in agglutination assays, immunoassays, and chromatography applications. The latexes used are highly cross-linked, rigid copolymers. The most common use of latex particles in these applications is as the solid support capture and separation of the desired analyte. Styrene-butadiene copolymers with low cross- linking are frequently designated surface activators or film forming particles. These preparations behave as particles in solution but upon contact with a surface dry to a conformable film. These film forming styrene- butadiene copolymers may contain a wide variety of functional binding groups. Groups which will control the hydrophilicity of a surface have also been described (U.S. Patent 4,835,211). In addition to styrene-butadiene, these copolymers may also contain isoprene, vinyl, acrylic, methacrylic, vinyldiene chloride, and etc. These material have found utility in agents to increase the cure rate of adhesives (U.S. Patent 3,836,377), as a binder polymer in paint formulations (U.S. Patent 4,683,269; U.S. Patent 4,391,928), as protective coatings (U.S. Patent 4,902,733; U.S. Patent 4,925,893; U.S. Patent 4,683,260; U.S. Patent 3,962,167; U.S. Patent 4,781,948; U.S. Patent 4,636,437; U.S. Patent 4,537,926; U.S. Patent 4,510,204), and in the production of toning films (U.S. Patent 4,806,451). Rigid polystyrene particles have been used for signal generation by incorporation of a dye. These particles are simply an alternate method for the introduction of a tag or label for signal generation. Use of colored or dyed latexes for agglutination assays and for membrane based assay have been extensively utilized( for a review see L.B. Bangs, American Clinical Laboratory News, May 1990) . As in previous cases, the primary requirement for these particles is that they maintain their structure for visualization in agglutination assays and do not distort to block the pores of the membrane-based tests. A wide range of chemistries may be introduced into the polystyrene-butadiene copolymers, an excellent review of the types of functional groups which have introduced into latexes is presented in L.B. Bangs, American Clinical Laboratory News, May 1990. Groups such as carboxylic acid, primary or aliphatic amines, aromatic amines, aniline, chloromethyl, amide, aldehyde, thiol, hydroxyl, nitrile, or epoxy have been used for the covalent attachment of receptive material or one member of interacting species to the surface of the polymer. This attachment can be accomplished through direct reaction of the functional group with the receptive material or may rely on modification of the functional group to a new reactive group with a specific desired chemistry. An example of the latter is the modification of an amide containing latex with hydrazide prior to attachment of an antibody to the latex preparation, U.S. Patent 4,421,896. While covalent attachment provides specific advantages with certain interacting species, passive adsorption of the receptive material to the latex is frequently adequate. While latex particles or styrene-butadiene copolymers have been used as solid support for immunoassays and in some affinity chromatography applications and as the method for introduction of a tag or label, they have not been widely used in the direct generation of signal in any system beyond agglutination assays. These assays require a rigid well-defined latex particle or bead. The present invention describes the utility of film-forming latex or other film-forming particle preparations known as surface activators in the generation of a signal in thin film detection methodologies. SUMMARY OF THE INVENTION This invention describes a unique method for the amplification of signals in assay systems which rely on a thin film method. Such methods include, but are not limited to, ellipsometry, interference effects, profilimetry, scanning tunneling microscopy, atomic force microscopy, interferometry, light scattering total internal reflection, or reflectometric techniques. The materials selected for use in these types of systems must maintain some degree of particulate character in solution and must, upon contact with a surface or support, form a stable film. The film should be conformal to the test surface to maintain the desired smoothness or texture of the substrate. The characteristic texture of the surface will be dependent on the detection method employed. The material selected must also be capable of adhering, through covalent or passive interaction, a receptive material or one member of an interacting set of species. The receptive material must be adhered to the signal amplifying material or particle in a manner which preserves the reactivity and stability of the receptive species. The receptive material applied to the particle may be identical to, or matched to the receptive species immobilized on the substrate of the test surface. It has now been found that a method of amplifying can be provided by use of surface activation components, the signal generated by a detected analyte of interest through its reactive substance in a suitable medium which method comprises the steps of (1) providing a detection element having a substrate supporting at least one interactive receptive layer, said layer capable of supporting at least one other interactive layer, then combining therewith a second interactive receptive layer- forming substance comprising an analyte of interest and a film-forming surface activator attached to said analyte and forming a polymeric latex in a medium, then observing and measuring the visually enhanced and discernable signal in the receptive layers. In a preferred method, the surface activator is a film-forming latex having a specific gravity nearly the same as water and is a high cross-linked copolymer of styrene-butadiene, preferably having water soluble -COOH moieties. There are numerous methods by which the detection of the thus amplified signals can be made including ellipsometrically. This method can be practiced using an element embodying the present invention which element exhibits enhanced surface optical signals, that is surface activation, which originate from the intersection between an analyte of interest and at least a first and second receiving layer of material. This element comprises a substrate as will be described more fully hereafter, supporting a layer of a first receiving material upon whose surface is contacted with a mixture comprising a combination of substances including an analyte of interest and a surface-activator film-forming latex. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A number of different initial substrates, optical substrates, are suitable for use with this invention. These substrates include, but are not limited to, onocrystalline silicon-polished or lapped wafers, optical glasses, dark glass, metal, plastic, borosilcate glasses, etc. These substrates may be molecularly planar or may be textured to provide a property desired for the detection method employed. The initial substrate may be coated with additional materials to provide a desired physical property, such as the use of anti-reflection coatings on a silicon wafer to create an interference film for use in the direct visualization of the interaction of reactive species. Beyond the initial substrate and the films applied to enhance the performance of the detection system, additional layers of material may be required to anchor the reactive species or receptive material to the surface of the substrate. Once the substrate is activated or treated to accept the reactive species, the receptive material may be applied by passive or covalent attachment. Amplification is required when the size of the analyte is insufficient in and of itself to produce a signal that is directly observable with any of the thin film detection methods, or when extremely low concentration levels must be detected. The amplification method employed must maintain the thin film characteristics required for the detection technique. For maintaining the properties of the thin films the surface activator particles or the film- forming latex formulations present the properties which may be exploited. While the formulations were designed for use in paints or as protective coatings, they share the attributes of the latexes more commonly used in detection schemes for various biological materials. In general, a thin film assay that requires amplification would consist of a substrate whose properties and characteristics are determined by the type of detection method used, optionally a secondary optical material, optionally an activation or adhering layer, a layer of receptive material, a sample of matrix appropriate to the analyte of interest, a reactive species coated film-forming particle, and any sample preparative reagents that may be required. A general assay protocol would require that the sample suspected of containing the analyte would be processed through any treatment necessary, such as extraction of a cellular antigen, and would then be mixed with the secondary or amplification reagent. An aliquot of this mixture would be applied to the receptive material coated substrate. After an appropriate incubation period, the unbound material would be separated from the reacted film by either a physical rinse/dry protocol or with a device contained rinse/dry step. The signal would then be visually or instrumentally interpreted. The introduction of the secondary or amplification film-forming particle could be by addition of a reagent to the sample, as a lyophilized material in the sample collection or application device, or embedded in an assay device. The production of the amplifying film-forming particles requires the selection of a film-forming particle or surface activator which provides sufficient particle density, is compatible with the secondary reactive species, and is of sufficient size (in solution) to increase the apparent thickness or density of the captured analyte. The secondary receptive material coated to the surface activator, may be identical to the receptive material immobilized to the substrate, or may be complementary to the receptive material. Appropriate receptive materials include, but are not limited to, antibodies, monoclonal or polyclonal; enzymes, their substrates or inhibitors; bacterial, viral, or hormonal receptors; DNA of synthetic or natural origin; RNA of synthetic or natural origin; or any reactive species for which there exists a corresponding reactive member(s) . The secondary reactive species would include any additional reactive species that reacts with the analyte without affecting the interaction of the analyte with the immobilized receptive material. The secondary reactive species may be immobilized on the surface activator particles by incubation at the appropriate temperature for a period of time. The temperature selected will be influenced by the chemistry utilized to attach the secondary reactive species to the particle, the nature of the reactive species, and the composition of the particle. In addition to the temperature, length of incubation, chemistry of immobilization, the buffer composition (pH and ionic strength and pH) and the amount of secondary reactive material must be optimized to the particular application. The specific examples given are intended to be representative of the type of method(s) used for the production of the film-forming amplification reagent. The conditions described are not intended as limitation in the preparation of such amplification reagents. EXAMPLE 1 Surface Activator particles were purchased from Bangs Laboratories, Carmel, In. ; Amide Particles: Lot Numbers L910108A (SA7-015/758) or L901015J (B7-015FF/181) ; or Carboxylate Particles: Lot Number L9004108 (SA1- 015/787). TC3, TC3X, TC7, and TC7X are similar fil - forming particles which were purchased from Seradyn, Inc. , Indianapolis, IN. All of the TC designated preparations were carboxylic acid containing styrene-butadiene copolymers. Of these preparations, only the TC7 particles were extensively examined. The TC3 preparations produce a very opalescent film and were not used with this detection application. Seradyn particles examined were TC-7A Product Number CML, Lot Number 1K30; TC-7X Lot Number 1M92, TC-7 Lot Number 1V18 (F040690) , TC-3X Lot Number 1R35, and TC-3 Lot Number 1J44. The surface activator preparations offer more flexibility in the chemistries of immobilization as both carboxylate and amide particles are available. The amide particles are readily converted to the hydrazide particle as described in U.S. Patent 4,421,896. In this study, monocrystalline silicon, virgin test wafers were purchased from Addison Engineering, Inc. , Sunnyvale, CA. These wafers were coated with a T-polymer siloxane (Petrarch Systems, Bristol, PA, Catalog Number PS401, Lot Number) by application of 300 microliters of l:300(v/v) dilution in 2-methyl-2-butanol of the stock siloxane using s spin coating device, a technique well known to those skilled int he semiconductor art. The T-polymer was cured to the surface of the wafer by a heat treatment for 120 minutes at 120 β C. The activated substrates were placed in a solution containing 20 micrograms/ml of a rabbit polyclonal antibody to Streptococcus Group A antigen (Medix Biotech, Inc. , Foster City, CA, Catalog Number V-872-01R, Lot Number M-11206) , in 0.1 M HEPES buffer, pH = 6.0. Wafers were submerged in the antibody solution for 60 minutes at 25 β C, removed, rinsed with deionized water, and dried under a stream of nitrogen. A thickness or optical density change is directly observed by examining a reacted wafer with the Sagax Comparison Ellipsometer or other ellipsometric techniques and comparison to a measurement made are the same wafer before reaction. Table 1 summarizes the results obtained with variously coated film-forming particles. Table 1 indicates the buffer condition, the antibody concentration, particle concentration, and the addition of any blocking materials if necessary. The antibody used for coating of the film- forming particles was the same as that used as the receptive material on the ellipsometric wafer. Antibody was coated to the surface of the particle by incubation of the mixtures at 25°C for 26 hours. TC7 preparations were examined as dilutions of the stock solutions, dialyzed against water prior to antibody treatment, and treated with a mixed bed resin prior to reaction with antibody. Covalent attachment of the antibody was examined with the carboxylate particles by the addition of a 1% final concentration (w/v) of a carbodiimide (l-cyclohexyl-3-(2- morpholinoethyl-carbodiimide metho-p-toluenesulfonate; Aldrich Chemical, Co., Milwaukee, WI, Catalog Number CIO,640-2, Lot Number 09915PW) . The carbodiimide was added prior to the addition of the antibody. Hydrazide treated amide particles were treated with a final concentration of 0.05% glutaraldehyde immediately before the addition of antibody to provide covalent attachment of the antibody onto the surface of the particle. Particles were coated with antibody in a 0.01 M Phosphate Buffered Saline at pH = 7.2 unless otherwise denoted. The Strep A antigen preparation used for the production of the positive control was purchased from Medix Biotech. This antigen was diluted into a mixture of one part 2M NaN0 2 , one part 2M Acetic Acid, and one part 0.66N NaOH to a 1:600 dilution level. The same preparation minus the antigen was used as the negative control. The secondary amplifying reagent was mixed in varying ratios, as designated in Table 1, with the positive and negative controls and five microliters were applied to the surface of the antibody coated wafer. The samples were incubated for 2 minutes at 25°C, rinsed, and then dried under a stream of nitrogen. Reacted wafers were examined with the Sagax Ellipsometer and thickness as a change in intensity measured in millivolts, was recorded. ιnoo "*σ» 03 H i i i i i i I I I I I I I • • H < O 03 H H H -t H '- a tf C^ H H H ^ r-I H r-l -^l' H CQ H H N H H H H i-i H H H H H H H H Λo H t^ t^ o o o oo ooo oo o o o o Table 1 (cont'd) Values Amplifier % Solid Blocker Neq Pos SA COOH-Surfactant 5 117 91 SA N=N/GlutH0 4 55 101 SA 10 281 135 SA 10 242 111 SA 3 19 25 SA 4 41 63 SA 10 125 217 SA 3 66 55 SA 10 66 63 SA 3 0 613 SA 3 0 320 Amplifier to Sample Ratio. a The final concentration of hydrazide in this particle preparation was 3 M and antibody was coated to the particle in 50 mM MES, pH=6.0 and at 56°C for 30 minutes. These data demonstrate than an extremely high particle density in the amplifying reagent introduces a non-specific association of that reagent with the test surface. Addition of ancillary proteins or surfactant do not improve the performance of the antibody coated particles. The TC-7 particles being slightly more rigid than the Surface Activators do not perform as well for this particular application, however, in comparison with more traditional latex preparations the TC-7 particles demonstrate significant reactivity. Increased temperature appears to improve the level of antibody incorporation into the particle and thus reduces the level of free antibody. While free antibody was not removed from these particles, it may be advantageous to remove unassociated antibody by a technique such as ultra-filtration. The hydrazide derivatized amide particles appear to provide the best overall reactivity although the amide particles also perform well. The surface activator carboxylate particles do not perform as well as the amide particles. EXAMPLE 2 In this study, the amplifying film-forming particles were prepared as in Example 1, and the assay was conducted as described above. However, in these experiments, a visually interpretable substrate was coated with the antibody to Strep Group A antigen. The substrate employed was a monocrystalline silicon wafer which was lapped with 12-20 micron aluminum oxide particles, mean particle size 15 microns, to create a rough textured surface using a process well known to those skilled in the semiconductor industry. This substrate is coated with a thin film of silicon nitride by any number of different deposition techniques, known to those skilled in the art. The optical coating requirements are that a film of the optical material be very uniformly applied to a tolerance much higher than traditionally required for semiconductor processes. A silicon nitride film of 350 to 550 A is standard for this application, however, any film thickness can be utilized. Silicon nitride is not the only material that is suitable for this application. The T-polymer treatment of the optical slide was as described above. These results support the observations made in the ellipsometric system. A very high particle density provides for a clean negative, but does not generate a strong visual signal. A higher level of antibody incorporation into the particle produces a stronger signal than does a lower level of antibody. This study suggests that insufficient antibody coverage of the particle also allows for non-specific association of the particle with the test surface. A dilution of the positive control to 1:64 is clearly resolved from the negative control. * Amplifier to Sample Ratio. a Final hydrazide concentration is 3 M and antibody is added to the particles in 50 mM MES, pH = 6.0 at 56°C for 30 minutes. A 2.5M MOPS was used as the neutralizer in these experiments to provide a final pH of 8.0. EXAMPLE 3 In this study, the ellipsometric surfaces used were those described in Example 1. The system examined was designed to detect the thrombin/anti-thrombin III (AT-III) complex in human plasmas. A polyclonal anti-thrombin and a polyclonal anti-ATIII (Dako Corporation, Santa Barbara, CA) were used of the production of test surfaces and amplifying reagent, respectively. The t-polymer ellipsometric surface was coated with 20 μg/ml of the anti- thrombin antibody in a 0.1 M Na 2 HP0 buffer at pH = 3.0. Secondary or Amplifying Reagent Preparation This secondary antibody reagent was produced using a modification of the attachment protocol described in Examples 1 and 2 for the hydrazide derivatized surface activators and requires the incubation of the particle at final density in water of 0.8% particle with 70 μg/ml of anti-AT III antibody. Assay Run Protocol Standards were prepared by reconstitution of the purified Thrombin Anti-Thrombin III complex in normal human plasma and then dilution with that plasma in four-fold dilutions to produce the standards for the range examined. Standards were then diluted 1:10 with normal saline. These solutions were mixed 1:1 with the secondary reagent and 10 microliters were applied to the anti-thrombin coated surface. The spots were incubated for 5 minutes at room temperature (25°C), then rinsed with deionized water and dried under a stream of nitrogen. These reacted slides were evaluated with a modification of the Sagax Comparison Ellipsometer which allowed acquisition of the data with a Compaq 386 computer. Samples values were extrapolated from the standard curves, Table 3. Table 3 TAT Plasma Standard Curve * The normal plasma was demonstrated to have a value of 3.3 ng/ml based on a commercially available ELISA kit. This residual TAT concentration was added to the standard levels to provide accurate standard concentrations. For comparison with the preferred amplifying reagent, a 70 μg/ml dilution of the anti-ATIII antibody was prepared in a commercially available conjugate diluent and examined with the plasma controls described above. Diluted antibody was mixed with the sample 1:1 as previously described. The assay conditions and procedure were constant. Plasma were used without dilution. Table 4 Concentration Ellipsometric Value fng/ml) fmV) 3.3 0.0 4.46 5.0 40.3 58.0 Very little resolution or dynamic range was obtained by use of the secondary antibody at the same concentration as was incorporated into the amplifying reagent. The performance of the amplifying reagent provides more consistent resolution of the standards. Saturation was reached for any concentration beyond the final concentration given in Table 4. Dilutions of 24 μg/ml and 48 μg/ml of an anti- ATIII antibody conjugated HorseRadish Peroxidase (HRP) were made in conjugate diluent. The plasma standards were mixed with the diluted conjugate preparations 1:1 and the assay protocol described above utilized. Table 5 shows representative assay performance for the 24 μg/ml conjugate and Table 6 for the 48 μg/ml, respectively. While slightly improved resolution is obtained with the conjugate preparations, the dynamic range is not achieved and the system saturates at concentrations above those given in the tables. Table 6 EXAMPLE 4 Secondary reagents were prepared by coupling either Key Hole Limpet Hemacynanin (KLH) or Ferritin to a polyclonal anti-Strep A antibody. Methods for conjugation of an antibody to KLH or Ferritin or any other protein are known to those skilled in the art. KLH should increase the signal due to its large size (MW = 1,000,000 daltons) . Ferritin is a large molecule but should have relatively high refractive index due to the 10 molecules of iron each molecule of Ferritin may carry. A high refractive index particle should favorably impact the visual detection system described in Example 2. Anti-Strep A antibody was coated to the Interference Slides as described in Example 2. The assay protocol described in Example 2 was followed but the extraction protocol used 2.5 M Trizma as the naturalizing reagent and the antigen preparation was from a different commercial source. Sample was mixed 1:1 with the secondary reagent. Visual signals generated at all ratios of sample to secondary reagent tested were extremely weak and difficult to interpret. The residual background signal made interpretation of a dilution of the neat positive control impossible to resolve from the negative control. Table 7 Secondary Visual Response Visual Response Reagent Negative Positive KLH +/- + Ferritin +/- + EXAMPLE 5 The surface activator amide particles, hydrazide treated as in Example 2, were treated with a dye which would impart a blue color to the particles. Excess dye was removed from the particles by dialysis and a 0.8% final solid preparation was reacted with the Medix anti-Strep A antibody for 30 minutes at 56°C, at a final antibody concentration of 75.0 μg/ml. Standards used in this study were as described in Example 4. The neutralizer used in this study was 2.5M MOPS to achieve a final pH of 8.0. Visual impression of the neat positive standard is comparable to that obtained with the initial surface activator hydrazide particle. Some slight level of non¬ specific binding was observed with the dyed SA/Hydrazide particle. While various forms and modifications of the invention have been disclosed, it will be appreciated that the invention is not limited thereto but encompasses all expedients and variations falling within the scope of the claims.

Claims: CLAIMS We Claim: 1. In a thin film detection system, a method of amplifying the signal generated by a detected analyte of interest reactive substance in a medium which comprises, providing a detection element having a substrate supporting at least one interactive receptive layer, said layer capable of supporting at least one other interactive layer, combining therewith a second interactive receptive layer-forming substance comprising an analyte of interest and a film-forming surface activator attached to said analyte and forming a polymeric latex in a medium, observing and measuring the visually enhanced discernable signals in said layers. 2. The method of Claim 1, wherein the surface activator is a film-forming latex. 3. The method of Claim 2, wherein the latex is a high, cross-linking styrene-butadiene copolymer. 4. The method of Claim 3, wherein the latex is covalently attached to the analyte of interest employing water soluble moieties. 5. The method of Claim 1, wherein the measurements are made ellipsometrically. 6. An element which exhibits enhanced surface optical signals emanating from the interaction between an analyte of interest and at least a first and second receiving material, said element comprising a substrate supporting a layer of a first receiving material upon whose surface is contacted with a mixture comprising a second receiving material further comprising a combination comprising an analyte of interest and a surface activator film-forming latex. 7. The element of Claim 6, wherein the surface activator is a high cross-linking styrene-butadiene copolymer. 8. The element of Claim 7, wherein the surface activator is attached to the analyte of interest employing water soluble moieties.

Inventors: BOGART GREG, ETTER JEFFREY, MAUL DIANA, PETERSON TAMMY, ZAPP LORETTA, BOGART, GREG, ETTER, JEFFREY, MAUL, DIANA, PETERSON, TAMMY, ZAPP, LORETTA
Applicants: BIOSTAR MED PROD, BIOSTAR MEDICAL PRODUCTS, INC.
Application year: 1992
Main classification: G01N33
IPC codes: G01N33