Microplate for assays using light measurement

Abstract: A microplate (10) forms a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission. The plate (10) comprises an upper plate (11) forming the side walls (13) of the sample wells, the side walls (13) being opaque so that light cannot be transmitted between adjacent wells through the side walls, (13) and a lower plate (12) forming the bottom walls (14) of the sample wells, the bottom walls (14) being transparent to allow the transmission of light therethrough. Bands of opaque material are provided within the lower plate (12) and surrounding each well to block the transmission of light between adjacent wells through the lower plate (12).

Description: Field of the Invention The present invention relates generally to multi-well sample trays which are commonly referred to as microplates and which are used to hold a large number (e.g., 24, 48 or 96) of samples to be assayed by various techniques such as scintillation counting, luminometry, kinetics etc. This invention is particularly concerned with microplates for use in assaying techniques which require the emission of light from the sample, as occurs in scintillation counting, fluorimetry and luminometry, or the transmission of light through the sample. Background of the Invention When microplates are used to hold samples to be assayed by techniques which are dependent on light emissions from the sample, it is important to avoid light transmission between adjacent samples, i.e., "crosstalk." Such crosstalk is extremely undesirable because it means that the photons detected in any particular sample well might not have originated from the particular sample in that well, and the purpose of the assaying technique is to obtain a unique measurement for each individual sample that is representative of only that sample. In certain applications, it is desirable to have a transparent wall in the bottom of the sample well. For example, when coincidence measurement is used, it is desirable to have a first photodetector at the top of the well, which is normally open, and a second photodetector at the bottom of the well, which is normally closed. Of course, photons can be detected at the bottom of the well only if the wall of the well is transparent. Even when light measurements are not made at the bottom of the well, if is often desirable to have a transparent well wall to allow microscopic viewing of adherent cells within the well. US-A-44 31 307 discloses a cuvette-set matrix having unitary transparent plate forming the side walls and bottom walls of each well. To prevent optical cross-talk between adjacent cuvettes, limiters are arranged between the cuvettes or the wall of the cuvettes, which are except for the bottom part, impervious to the beams of measurement. In this case, special inserts forming the bottom wall of each well are used to permit the light beam from a light source to reach a light detector arranged below the bottom wall. Instead, a protective layer can be deposited on the side walls of the wells in case the plate forming the side walls and bottom walls of each well is transparent. US-A-49 56 150 discloses a microtitre stick having a solid plastic body and a row of wells. The side wall of the wells of frosted or stippled to eliminate optical colour interference between adjacent wells. The bottom walls of the wells are formed by a series of transparent lens portions. With respect to US-A-44 31 307, it is an object of the present invention to provide an improved microplate which can be rapidly and efficiently manufactured, whereby viewing of the sample and/or the measurement of light emission from the sample is permitted and yet cross-talk between adjacent wells is avoided. In accordance with the present invention, the fore-going object is realised by providing a microplate with the features of claim 1. Moreover, according to claim 11 the advantageous use of the microplate according to the invention in a method of assaying samples is disclosed. Wherein specially the light emitted from the samples in the sample wells is measured. Further advantages and embodiments of the invention are disclosed by the sub-claims and will become apparent upon reading the following detailed description and upon reference to the accompanying drawings. Brief Description of the Drawings FIG. 1 is an exploded perspective view of a microplate embodying the present invention: FIG. 2 is a top plan view of the upper portion of the microplate of FIG. 1; FIG. 3 is a bottom plan view of the upper portion of the microplate of FIG. 1; FIG. 4 is a top plan view of the lower portion of the microplate of FIG. 1; FIG. 5 is an enlarged section taken generally along line 5-5 in FIG. 1 and showing the two parts of the microplate before they have been joined to each other; FIG. 6 is an enlarged section similar to FIG. 5 but showing the two parts of the microplate after they have been joined; and FIG. 7 is an enlargement of a portion of FIG. 6. Description of the Preferred Embodiments Turning now to the drawings and referring first to FIG. 1, there is shown a microplate 10 formed from two molded plastic plates 11 and 12. The upper plate 11 forms the side walls 13 of the multiple wells of the microplate, and in the illustrative example the wells are arranged in an 8 x 12 or 4 x 6 matrix. The bottom plate 12 forms the bottom walls 14 of the wells, and is attached to the lower surface of the upper plate 11 by fusing the two plates together. As will be described in more detail below, the fusion is preferably effected by ultrasonic bonding. In order to confine light emissions to the well in which they originate, i.e., to prevent light transmission between adjacent wells, the upper plate 11 is formed from an opaque polymeric material so that light cannot be transmitted therethrough. For assaying techniques which require the detection of very small amounts of light, as in liquid scintillation counting, the pigmentation used to render the polymeric material opaque is preferably light in color so as to be highly reflective in order to ensure high counting efficiency with respect to the radioactive samples. To form an opaque light colored plate, a pigment having a high Albedo, preferably white, is added to the resin in an amount from about 2 to about 20 weight percent. If a greater amount of pigment is added, the resin becomes too viscous for injection molding. The white pigment is selected from the group consisting of titanium dioxide, zinc oxide, zinc sulfide and thithopone. Titanium dioxide is generally more chemically resistant to scintillation cocktail solvents. In certain types of luminescence and fluorescence assays it is preferred that the side walls 13 of the sample wells be non-reflective, in which case the upper plate 11 is preferably formed from a black or dark-colored polymer. The dark polymer may be formed by the addition of carbon black in amounts ranging from about 0.5 weight % to about 15 weight %. In contrast to the upper plate 11, the lower plate 12 is formed of a transparent polymeric material so that it forms a transparent bottom wall 14 for each sample well. This permits viewing of sample material through the bottom wall 14, and also permits light emissions to be measured through the bottom wall. The transparent bottom walls 14 may also be used to expose the sample to light from an external excitation source, while leaving the tops of the wells unobstructed for maximum detection area. Examples of suitable transparent polymers are clear polystyrene, polyacrylonitrile, polycarbonate, polyester, polymethyl pentene and acrylic materials. The transparent bottom walls of the wells are desirable in assaying techniques that measure light emitted from, or transmitted through, the sample in each individual well. Examples of such techniques are liquid scintillation counting, which counts light emissions produced by a radioactive sample in a liquid scintillator, and techniques which measure light emitted by luminescent labels, such as bioluminescent or chemiluminescent labels, fluorescent labels, or absorbence labels. These techniques use various types of light detectors, such as one or more photomultiplier tubes per well, solid state imaging devices with either lenses or fiber optic couplers, and fiber optic devices. For any given assaying technique, the polymeric material chosen to form the plate must be nonreactive with, insoluble in, and impervious to the materials contained in the samples to be used in the assay. One particularly preferred resin is a copolymer containing at least 50 weight percent of an unsaturated nitrile monomer and a second monomer which is capable of being copolymerized with the unsaturated nitrile monomer. These high nitrile resins have excellent transparency, rigidity, processability and gas barrier resistance to oxygen, carbon dioxide and other gases. The resins are highly chemically resistant to solvents such as benzene, toluene, xylene, 1, 2, 4-trimethylbenzene (pseudocumene), alkobenzenes, diisopropyl napthalene, phenylxylylethane (PxE), heptane and ethyl acetate. One or more of the aforementioned solvents are usually present in liquid scintillation cocktails. Additionally, these resins form a microplate that does not deteriorate when exposed to ultraviolet light during storage. Preferably, the unsaturated nitrile monomer of the above resins is selected from the group consisting of acrylonitrile and methacrylonitrile. The monomer capable of being copolymerized with the unsaturated nitrile is an ethylenically unsaturated copolymerizable monomer selected from the group consisting of alkyl acrylates, alkyl methacrylates, acrylic acid or methacrylic acid. According to one embodiment of the invention, the resin is a rubber modified acrylonitrile-methylacrylate copolymer containing about 75 weight percent acrylonitrile and about 25 weight percent methylacrylate. Such a rubber modified copolymer resin is commercially available under the trademark Barex 210-I® resin manufactured by British Petroleum Chemicals Corporation. For applications where the solvent resistance of the above-described copolymers is not required, polystyrene is a very cost-effective polymer that can be readily molded to form the plates 11 and 12. To form a barrier to the transmission of light between adjacent wells via the transparent bottom plate 12, a downwardly protruding bead 20 is formed around the entire circumference of each well opening on the bottom surface of the upper plate 11. As can be seen in FIG. 7, this bead 20 has a tapering cross-section, so that it is narrower at its bottom than at the top. When the two plates 11 and 12 are pressed together, each bead 20 fits into a circular groove 21 formed in the top surface of the lower plate 12. The vertical dimension of each bead 20 is at least as great as the depth of the groove 21, so that when the two plates are pressed together, the lowermost surfaces of the beads 20 are pressed into firm contact with the lower surfaces of the grooves 21. The two plates 11 and 12 may be joined together by various processes, but the preferred process is ultrasonic bonding. In this process the two plates are held firmly pressed together while they are bonded by the application of ultrasonic energy which causes the contacting surfaces to fuse together. Because of the shape of the beads 20, they function as energy directors, causing the energy to be concentrated at the interface between the beads and the corresponding grooves and thereby ensuring that the beads 20 are melted within the grooves 21. This melting fusion causes the opaque polymer of the beads 20 to penetrate beneath the upper surface of the transparent lower plate 12. This forms a circular band 23 of opaque polymer around the transparent bottom wall of each well, thereby blocking the transmission of light between adjacent wells via the transparent bottom plate 12. The beads 20 and grooves 21 should penetrate through 25% to 75% of the thickness of the transparent bottom plate 12 for effective reduction of optical cross talk. In a preferred embodiment, the bead and groove have a vertical dimension of 0,635 mm (0.025 inch) and the bottom plate 12 has a thickness of 1,52 mm (0.060 inch). The inner edge of each groove is spaced 0,254 mm (0.010 inch) from the side wall of the corresponding wall. Microplates formed in this manner have been found to significantly reduce optical and liquid crosstalk. For example, when used in liquid scintillation counting, liquid crosstalk is eliminiated and optical crosstalk is reduced from 2% or more to about 0.2% by the addition of the non-transmissive bands 23. For the purpose of attaching the two plates 11 and 12 to each other, while at the same time forming an effective seal against liquid cross talk, a grid 30 of beads 31 is formed on the top surface of the lower plate 12. A similar grid 32 of grooves 33 is formed in the bottom surface of the upper plate 11 for receiving the bead grid 30. The height of the beads 31 is made substantially greater the the depth of the grooves 33 to ensure that the beads 31 are thoroughly melted to fill the grooves 33. For example, in a preferred embodiment the vertical dimensions of the beads 31 and the grooves 33 are 0,66 mm (0.026 inch) and 0,18 mm (0.007 inch), respectively. With these dimensions, the application of ultrasonic energy thoroughly melts the beads 31. The preferred ultrasonic bonding process may be effected by applying the energy to the lower plate 12 through a transducer or "horn" having a flat surface of about the same dimensions as the plate 12, with multiple recesses located in the same regions as the well holes in the upper plate 11. The recesses help to concentrate the ultrasonic energy in the regions between the wells. An alternative to the ultrasonic bonding process described above is to mold the opaque upper plate 11 with the depending beads 20, and then mold the transparent lower plate 12 directly onto the bottom of the upper plate 11. The opaque beads 20 are thus embedded in the lower plate 12 to form the desired non-transmissive bands 23 between adjacent wells. It is also possible to use solvent or adhesives to fuse the two plates together. While the invention has been described with particular reference to certain specific embodiments, it will be understood that various modifications may be made without departing from the spirit or scope of the invention. For example, the grooves in the top surface of the lower plate may be filled with opaque material from a source other than the beads on the lower surface of the upper plate. For example, the grooves could be filled with particulate or liquid opaque material. Also, in cases where a rigid bottom plate is not required, transparent bottom walls may be formed by applying a sheet of transparent film, such as polyester film, to the lower surface of the top plate 11 from which the beads 21 have been omitted. Such a film may be attached to the top plate 11 by means of adhesive, for example. Alternatively, the upper surface of the transparent film may be coated with another polymer such as polyethylene that will melt into the top plate.

Claims: A microplate (10) forming a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission, side walls (13) of said sample wells being opaque so that light cannot be transmitted between adjacent wells through said side walls, bottom walls (14) of the sample wells being transparent to allow the transmission of light therethrough and bands (23) of opaque material disposed between the bottom walls (14) of adjacent pairs of sample wells characterized in that the side walls (13) of the sample wells are formed from a unitary upper plate (11), and the bottom walls (14) of the sample wells are formed from a unitary lower plate (12). Microplate according to claim 1, characterized in that said bands (23) of opaque material surround each well . Microplate according to claim 1 or 2, characterized in that said bands (23) of opaque material are formed within said lower plate (12). Microplate according to one of the previous claims, characterized in that said bands (23) of opaque material are formed by melting together the opaque material of said upper plate (11) and the transparent material of said lower plate (12) in preselected regions. Microplate according to one of the previous claims, characterized in that said upper plate (11) includes depending beads (20) on the lower surface thereof, each bead surrounding one of said wells, and said lower plate (12) includes grooves (21) in the upper surface thereof for receiving said beads (20). Microplate, according to claim 5, characterized in that said beads (20) on said upper plate (11) are fused to said lower plate (12) in said grooves (21). Microplate according to claim 6, characterized in that said beads (20) are fused to said lower plate (12) by ultrasonic bonding. Microplate according to one of the previous claims, characterized in that said upper plate (11) is formed of a polymer which includes from about 2 to about 20 weight percent white pigment for opacity and reflectivity. Microplate according to claim 8, characterized in that said pigment is selected from the group consisting of titanium dioxide, zinc oxide, zinc sulfide, thithopane and black pigment containing 0.5% to 15% of carbon black. Microplate according to one of the previous claims, characterized in that said upper plate (11) is formed of a polymer which includes from about 0.5 to about 15 weight percent carbon black. A method of assaying samples, comprising the steps of providing a microplate (10) according to anyone of claims 1 to 10 placing the samples in the sample wells, said transparent bottom walls (14) permitting said samples to be viewed therethrough, conditioning the samples in said sample wells to emit light, and measuring the light emitted from the samples in said sample wells.

Inventors: EFFERTZ BERNARD S, KOLB ALFRED J, MANNS ROY L, EFFERTZ, BERNARD S., KOLB, ALFRED J., MANNS, ROY L.
Applicants: PACKARD INSTRUMENT CO INC, PACKARD INSTRUMENT COMPANY, INC.
Application year: 1992
Main classification: B01L3
IPC codes: G01N21, B01L3