ANTI-TUMOR PLATINUM COMPLEXES

Abstract: Compounds of general formula (1): (am)2Pt O-CO-C6H4-NH2(p), (Cl)n (HCl)n1 (ROH)m wherein am is a monovalent amine ligand, selected from NH3, linear or branched C1-5 alkylamine, cyclopentylamine, cyclopropylamine or cyclohexylamine; or the two am groups form a linear or cyclic C2-8-alkyl-1,2 or 1,3-diamine; R is hydrogen or C1-3 alkyl; X is hydrogen, methyl or ethyl; n1, n and m are independently zero or the integer 1.

Description: ANTI-TϋMOR PLATINUM COMPLEXES The present invention refers to platinum(II) complexes of formula (1) (a m ) 2 Pt 0-CO-C 6 H 4 -NH 2 (p), (Cl) n (HCl)^ (ROH) m (1) wherein: a_m is a monovalent amine ligand,' selected from NH3_., linear or branched C, - alkylamine, cyclopentylamine, cyclopropylamine or cyclohexylamine; or the two a groups form a linear or cyclic C- g-alkyl-1,2 or 1,3- diamine; R is hydrogen or C, , alkyl; X is hydrogen, methyl or ethyl; n, , n and m are independently zero or the integer 1. A linear or branched C, - alkylamine is preferably isopropylamine, propylamine or n-pentylamine; the linear or branched C, , dialkyla ine is preferably diisopropylamine. Examples of linear C 2 „-alkyl-l,2- or 1,3-diamine include 1,2-ethylenediamine, (R, S, racemic or meso)- butane-l,2-diamine, 1,3-propylenediamine; examples of cyclic diamine comprise R, S, racemic or meso cyclopen- tane-1,2-diamine; R, S, (racemic or meso) cyclohexane- 1,2-diamine; 1,1-bis-aminomethyl-cyclopentane; l^l-bis- aminomethylcyclohexane. When m is 1, the compounds (1) are solvates with water or alcohols. When n is 1, the compounds (1) are addition salts. Particularly preferred compounds are: cis-diamino-chloro-(4-amino)benzoate-platinum(II) , cis-diamino-chloro-(4-amino)benzoate-platinum(II) hydrochloride, (1A) aquo-cis-diamino-chloro-(4-amino)benzoate-platinum(II) , (IB) cis-diamino-chloro-(4-amino)benzoate-platinum(II) mono methanol solvate, (1C). The compounds of formula (1) are prepared by a process comprising: a) the reaction of a dichloro platinum(II) complexes of formula (2) wherein am is a defined above, with at least one molar equivalent of p-aminobenzoic acid in the form of free acid or of carboxylate salt with a mono or bivalent metal, preferably alkali, alkaline-earth metal or silver, in a suitable hydroxylated solvent; b) the subsequent purification of a compound of formula (1)_ Preferred solvents are water, C, , alcohols or mixtures thereof. Water solutions or suspensions of reagents are particularly preferred. It is particularly preferred the use of lithium p- aminobenzoate which is more easily handled and J ^ ess toxic than the silver or barium salts. The reaction may be carried out by mixing water solutions of the reagents or adding a dichloro- platinum(II) of formula (2) as finely subdivided solid to a water solution of p-aminobenzoic acid in form of free acids or its salts. The mixture is stirred for a period ranging from some hours to some days, at temperature from the room temperature to the reflux temperature. Preferred temperatures are comprised from 50 to 80°C for a period from 1 and 24 hours. At least one molar equivalent of p-aminobenzoic acid or of its salt is used per mole of platinum complex (2); the use of an excess amount increases the reaction rate. The purification of compounds (1) may be carried out by precipitation as hydrochloride salts by dilution of an aqueous solution with concentrated hydrochloric acid; or by silica gel column chromatography or by repeated crystallizations from an alcoholic solvents, e.g. methanol. The dichloro platinum complexes (2) are commercially available or they are known or they may be prepared by known methods. The compounds (1), particularly in the salified form, are characterized by a good or very good water solubility (e.g.: 1A 7 mg/ml; 1C>3 mg/ml), and the solutions in water or in HCl 0.01 N have a remarkable stability (12-24 hours at least at room temperature). The compounds (1) have a low toxicity; for example the LD 5 for IB and 1C is higher than 240 mg/kg vas surprisingly higher than 1200 mg/kg for 1A. The low toxicity is combined with a low or irrelevant nephrotoxicity as shown by the BUN value of 36±8 mg/100 ml, after a cumulative dose of 1200 mg/kg of compound 1A, and measured at the 8 day after the end of the treatment. The evaluation of the cytotoxic activities of the compounds (1) was carried out "in vitro" measuring, according to known methods, the inhibition of the colony formation, the cell viability (Trypan blu) and the incorporation of tritiated thymidine in tumoral and normal cells. For instance, compound 1A inhibits the "in vitro" growth of leukemia cells P388, K562 and Jurkat (ID 5Q measured by incorporation of tritiated thymidine at 48 hours of 11.4, 26.3 and 21.3 μM, respectively) and has significant cytotoxic activity on cells of solid tumors, such as ovary (SW626), breast (MCF-7 and MDA) , lung (A549), melanoma (SKMEL-26), microcytoma (GCLI) as well as on non tumoral stabilized EBV + cell lines, both B95-8 (marmoset) and from patients affected by acute or chronic mononucleosis. The "in vivo" cytotoxic activity of the compounds of the invention was determined by measuring survival 5 of BDF1 mice inoculated i.p. with 10 cells of P388, in comparison with untreated controls. The compounds (1) were administered at increasing doses, according to different therapeutic schemes. The efficacy of the compounds is dose-dependent and is strictly related to the cumulative administered dose. The compounds (1) are therefore particularly advantageous and useful for the treatment of tumors sensitive to platinum complexes both because of their high water solubility and of their intrinsic low general toxicity and nephrotoxicity. The effective dosage of the compounds of the invention may be determined by a skilled physician with known methods. The correlation between the dosages used in animals of various species and sizes and those used 2 in human (according to mg/m of body surface) is disclosed by Freirech, E.J. et al. , in Cancer Chemother. Rep., 5_0, 4, 219-244; 1966. Usually, doses from 1 to 1200 mg/kg of the complexes will be administered, with a dosage regimen changing according to various factors. The treatment regimen may be suitably changed according to the kind of tumor and to the patients conditions. The compound of the invention are preferably administered as sterile aqueous solutions, preferably by intravenous or intraarterial route although other administration routes may be convenient in particular cases. The pharmaceutical forms which may be used for the parenteral administration comprise sterile aqueous solutions or sterile powders for the extemporaneous preparation of the solutions as well as oily preparations by intramuscular or intraperitoneal administration. Other useful pharmaceutical forms may be syrup or similar liquid forms, as well as solid forms such as tablets, capsules or the like. The preparation of the compounds of the invention is shown in the following examples. EXAMPLE 1 0.66 mol of p-aminobenzoic acid (PABA) finely divided are added under stirring to a solution of cis- diamino-dichloro-platinum(II) (0.33 mol) in water (25 ml). The mixture is heated to 80 β C and, kept at this temperature for 20 hours under stirring. After filtration and cooling to room temperature, the mixture is added with 10 ml of concentrated hydrochloric acid. By subsequent cooling for 24 hours at 0-4 β C, a crystalline precipitate of cis-diamino-chloro-(4- amino)benzoate-platinu (II) hydrochloride 1A is separated, which is filtered. The crystalline product is washed with ethyl ether and dried at room temperature under vacuum. If desired, the product may be re-crystallized by treatment of a solution of 0.09 g of cis-diamino- chloro-(4-amino)benzoate-platinum(II) hydrochloride in water (15 ml) with cone. HC1 (6 ml) which, after cooling at 5-10 β C, yields an analitically pure sample, decomposing at 215-220°C. [Pt(NH 3 ) 2 (NH 2 'CgH 4 -COOH)Cl]Cl IR (KBr, 4000-200 cm "1 ): 3300-3000 (m) , 2600 (d) , 2460 (d) , 1705 (f), 1610 (m) , 1510 (d), 1235 (m) , 340 (d) . 1 H-N R (200 MHz, internal standard TMS, solvent DMSO) : (Ppra) 3.35. (q.s., 2H) ; 4.11 (q.s., 3H) ; 4.72 (q.s., 3H) ; 7.40 (d. , J = 8.5 Hz, 2 H) ; 7.87 (d. , J = 8.5 Hz, 2 H) ; 7.60-8.10 (s.a. , 1 H, exchanges with D 2 0) UV (H 2 0) max (nm) log E 205 4.09 238 4.06. EXAMPLE 2 An aqueous solution of cis-diamine-chloro-(4- amino)benzoate-platinum(II) hydrochloride is adsorbed on silica gel. By methanol elution and partial evaporation, a crystalline precipitate is separated of aquo-cis-diamino-chloro-(4-amino)benzoate-platinum(II) , lb, which is filtered and dried at room temperature under vacuum. EXAMPLE 3 Lithium hydroxide (2 mM, 0.048 g) is added to a suspension of PABA (2 mM, 0.274 g) in water (50 ml); the mixture is kept under stirring at room temperature until a clear and colourless solution is obtained which is added, in a single batch, with cis-diamino- dichloroplatinum(II) (1 mM, 0.3 g). The suspension is stirred at 50 β C until complete dissolution of the dichloro-platinum complex (about 1 hour); the heating is continued for a further hour. After hot filtration, the solvent is evaporated under vacuum to dryness, avoiding that the solution inner temperature exceeds 40 β C. The residue is purified by repeated suspension in methanol and subsequent methanol filtration so as to obtain pure cis-diamino-chloro-(4-amino)benzo_ute- platinum(II) mono methanol solvate, 1C.

Claims: CLAIMS 1. Compounds of general formula (1): (a m ) 2 P 0-CO-C 6 H 4 -NH 2 (p), (Cl) n (HCl)^ (R0H) m (χ) wherein: am is a monovalent amine ligand, selected from NH3, linear or branched C, g alkylamine, cyclopentylamine, cyclopropylamine or cyclohexylamine; or the two a groups form a linear or cyclic C« „-alkyl-l,2 or 1,3- diamine; R is hydrogen or C, , alkyl; X is hydrogen, methyl or ethyl; n,, n and m are independently zero or the integer 1. 2. Compounds according to claim 1 wherein a is selected from 1,2-ethylenediamine, 1,3-propylenedia- ine, (R,S)-cyclopentane-l,2-diamine, (R,S)-cyclo- hexane-1,2-diamine, 1,1-bis-aminomethyl-cyclopentane, 1,1-bis-aminomethylcyclohexane. 3. Compounds according to claim 1 or 2 wherein R is selected from hydrogen and methyl. 4. A compound according to claim 1 selected from cis-diamino-chloro-(4-amino)benzoate-platinum(II) , cis-diamino-chloro-(4-amino)benzoate-platinum(II) hydrochloride, (1A) aquo-cis-diamino-chloro-(4-amino)benzoate-platinum(II) , (IB) cis-diamino-chloro-(4-amino)benzoate-platinum(II) mono methanol solvate, (1C) . 5. A process for the preparation of compounds of claims 1-4, characterized in that a dichloro- platinum(II) complex of formula (2) wherein a_m is as defined above, is reacted with p- aminobenzoic acid or with its salts, in an hydroxylated solvent. 6. Pharmaceutical compositions containing, as an active principle, a compound of claims 1-4 in admixture with suitable excipients.

Inventors: ESPOSITO MAURO, ESPOSITO, MAURO
Applicants: IST NAZ RIC SUL CANCRO, ISTITUTO NAZIONALE PER LA RICERCA SUL CANCRO
Application year: 1992
Main classification: A61K31
IPC codes: C07F15