PHOSPHOLIPASE A2 INHIBITOR

Abstract: A novel thielocin derivative having a phospholipase A₂ inhibitory activity, represented by general formula (I), or a salt thereof, wherein R¹ to R⁸ represent each independently hydrogen, lower alkyl, lower alkoxy, hydroxy or halogen; E₁ and E₂ represent each independently hydrogen or an ester residue; m and n represent each independently an integer of 0 to 4; and -Y- represents a divalent group selected among those represented by the following formulae -CH₂CH₂-, -CH = CH-, (II), (III), (IV), (V), (VI), (VII).

Description: The present invention relates to a novel phospholipase A 2 inhibitor. In particular, the present invention relates to novel compounds exhibiting an inhibiting effect on phospholipase A 2 , which compounds are analogs of physiologically active substances, thielocins which are produced by microorganisms such as Thielavia terricola RF-143 belonging to the Thielavia genus. Phospholipase A 2 is an enzyme which exists in cells and secretory liquids, in particular, the venom of snakes, the pancreas of mammals, blood platelets of various animals, the arthritis exudate of higher animals, and elsewhere. The enzyme specifically hydrolyzes phospholipids. For example, the enzyme specifically hydrolyzes C-2 fatty acid esters of 1,2-diacylglycerol phospholipids to form lysoglycerophospholipids and fatty acids. Phospholipid A 2 exhibits toxicity on nerves, muscles and the heart, and anticoagulant action in association with the above enzymatic action, and it is generally said that the enzyme may induce, for example, convulsing, hypotonia, haemolysis, and edema. Furthermore, the enzyme can also be responsible for other clinical symptoms including inflammation. It should be noted that phospholipase A 2 is recognized to be one of the phlogogenic substances in humans. If the enzymatic activity of phospholipase A 2 , which is a phlogogenic substance, can be inhibited, various diseases caused by or associated with the enzymatic activity can probably be treated. Based on such an assumption, substances such as mepacrine and p -bromophenacyl bromide have already been developed, and the applicants have also claimed and described novel phospholipase A 2 inhibitors in Japanese Patent Publication (kokai) No. 286088/1990 and Japanese Patent Application No. 234955/1990. However, it is desirable to develop additional phospholipase A 2 inhibitors, because types of the phospholipase A 2 molecules are varied and the activity of one molecule is not the same as another due to the difference of the structure of the molecules. The applicants have filed the Japanese patent applications which claim and describe for example, thielocin A 1 α, A 1 β, which is produced by Thielavia terricola RF-143 (Japanese Patent Application No. 109939/1989, and others). Now the applicants have chemically synthesized various new thielocin derivatives which are useful as medicines. Specifically, the present invention relates to thielocin derivatives of the formula: wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 . R 7 , and R 8 are independently hydrogen, lower alkyl, lower a;koxy, hydroxy, or halogen; E 1 and E 2 are independently hydrogen, or an ester residue; m and n are independently an integer of 0 to 4; -Y- is a bivalent group which is selected from the following radicals: -CH 2 CH 2 - , -CH=CH- , provided that m + n is not zero, when Y is -CH 2 CH 2 -, -CH=CH-, wherein X is a single bond, CH 2 , O, S, SO, or SO 2 ; R 9 and R 10 are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, or R 9 and R 10 may be combined together to form methylene, ether, sulfide, sulfinyl, or sulfone; R 9' and R 10' are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, or R 9' and R 10' may be combined together to form methylene, ether, sulfide, sulfinyl, or sulfone; R 11 is hydrogen or lower alkyl; R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , R 26 , R 27 , R 28 , R 29 , R 30 , and R 31 are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, provided that one of R 9 , R 15 , R 16 , R 17 and R 18 , one of R 10 , R 12 , R 13 and R 14 , one of R 9' , R 23 , R 24 , R 25 and R 26 , one of R 10' , R 19 , R 20 , R 21 and R 22 , and one of R 27 , R 28 , R 29 , R 30 and R 31 are a single bond; R 32 , R 33 , R 34 , R 35 , and R 36 are independently hydrogen, lower alkyl, lower alkoxy, hydroxy, halogen, or Z, and R 37 is hydrogen or an amino-protecting group, wherein Z is a single bond or a bivalent group of the formula: R 38 , R 39 , R 40 , R 41 , and R 42 are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, provided that one of R 32 , R 33 , R 34 , R 35 and R 36 is always Z, and when Z is not a single bond, one of R 38 , R 39 , R 40 , R 41 and R 42 is a single bond, or when two or more of R 32 , R 33 , R 34 , R 35 and R 36 are Z, only one of Zs and R 38 , R 39 , R 40 , R 41 and R 42 is a single bond; or the salts thereof. Several terms used in the present specification are defined below: The term "lower alkyl" refers to C 1 -C 6 alkyl such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl and hexyl. The term "lower alkoxy" refers to C 1 -C 6 alkoxy such as methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, tert-butoxy, pentoxy and hexyloxy. The term "halogen" refers to chlorine, bromine, iodine, or fluorine. The term "ester residues" refers to an alkyl having 1 to 8 carbon atoms (e.g. methyl, methoxymethyl, ethyl, ethoxymethyl, iodoethyl, propyl, isopropyl, butyl, isobutyl, ethoxyethyl, methylthioethyl, methanesulfonylethyl, trichloroethyl and t-butyl), an alkenyl having 3 to 8 carbon atoms (e.g. propenyl, allyl, prenyl, hexenyl, phenylpropenyl and dimethylhexenyl), an aralkyl having 7 to 19 carbon atoms (e.g. benzyl, methylbenzyl, dimethylbenzyl, methoxybenzyl, ethoxybenzyl, nitrobenzyl, aminobenzyl, diphenylmethyl, phenylethyl, trityl, di-t-butylhydroxybenzyl, phthalidyl and phenacyl), an aryl having 6 to 12 carbon atoms (e.g. phenyl, toluyl, methoxyphenyl, diisopropylphenyl, xylyl, trichlorophenyl, pentachlorophenyl and indanyl), an ester formed with a N-hydroxyamino compound having 1 to 12 carbon atoms (e.g. esters formed with acetone oxime, acetophenone oxime, acetaldoxime, N-hydroxy succinimide and N-hydroxy phthalimide), a hydrocarbonated silyl having 3 to 12 carbon atoms (e.g. trimethyl silyl, dimethyl methoxy silyl and t-butyl dimethyl silyl), a hydrocarbonated stannyl having 3 to 12 carbon atoms (e.g. trimethyl stannyl), a mono-oxygenated alkyl having 2 - 15 carbon atoms e.g. a straight, branched, cyclic or partially cyclic alkanoyloxyalkyl such as acetoxymethyl, acetoxyethyl, propionyloxymethyl, pivaloyloxymethyl, pivaloyloxyethyl, cyclohexanacetoxyethyl, and cyclohexanecarbonyloxycyclohexylmethyl), an alkoxycarbonyloxyalkyl having 3 to 15 carbon atoms (e.g. ethoxycarbonyloxyethyl, isopropoxycarbonyloxyethyl, isopropoxycarbonyloxypropyl, t-butoxycarbonyloxyethyl, isopentyloxycarbonyloxypropyl, cyclohexyloxycarbonyloxyethyl, cyclohexylmethoxycarbonyloxyethyl, and bornyloxycarbonyloxyisopropyl), an alkoxyalkyl having 2 to 8 carbon atoms (e.g. methoxymethyl, and methoxyethyl), 2-oxacycloalkyl having 4 to 8 carbon atoms (e.g. tetrahydropyranyl and tetrahydrofuranyl esters), a substituted aralkyl having 8 to 12 carbon atoms (e.g. phenacyl and phthalidyl), an aryl having 6 to 12 carbon atoms (e.g. phenyl, xylyl, and indanyl), an alkenyl having 2 to 12 carbon atoms (e.g. allyl, and (2-oxo-1,3-disloyal) methyl). The protected group moiety may have any further substations. An "amino-protecting group" includes those which are usually used in the art, and the preferred amino-protecting groups include acyl derivatives such as benzoyl, acetyl, formyl and trifluoroacetyl, urethane type derivatives such as benzyloxycarbonyl, t-butoxycarbonyl, isopropoxycarbonyl and methoxycarbonyl, or alkyl derivatives such as allyl, benzyl, trityl, and tetrahydropyranyl. The compounds of the present invention can form salts with a metal such as an alkali metal (e.g. sodium or potassium), or an alkaline earth metal (e.g. calcium), which may form a salt with a carboxylic acid. The compounds of the present invention can be prepared by coupling a dicarboxylic acid derivative of the formula: R O C - Y - C O R' wherein R and R' are independently hydroxy, halogen, or an ester residue, with an alcohol of the formula: wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , E 1 and E 2 , m, and n are as defined above, and if necessary, conducting a deesterification. The present compounds can be formulated into oral or external preparations in association with various carriers. The dose of the compounds will differ depending on the intended treatment effect, the administration route, and age and body weight of particular patients, and therefore it is difficult to define the dose in general. On the whole, a daily dose may be about 0.1 mg to about 500 mg, preferably 0.5 mg to about 100 mg in the case of oral administration. On administration, the above dose may be divided into one to five portions. Typical examples of the compounds of the present invention, which are shown in the above formula are illustrated below: According to a further aspect of the present invention, there is provided another class of the following compounds which also exhibit phospholipase A 2 inhibitory effect: All of the compounds of the present invention can be prepared by various methods which are known in the art. The following Examples and Preparations are provided to further illustrate the process for preparing the compounds of the invention. [Step 1] ( 1 → 2 ) A mixture containing the compound 1 (5.0 g, 25.5 mM), which is known in the literature, PhCH 2 Br (3.2 ml, 25.5 mM x 1.06), K 2 CO 3 (8.8 g, 25.5 mM x 2.5), and 200 ml of acetone was stirred at room temperature for 23 hours, and then the resultant solid was filtered off. The filtrate was distributed between ethyl acetate and 2 N hydrochloric acid, and the organic phase was washed with water, dried over Na 2 SO 4 , and then concentrated in vacuo to yield the crude compound 2 . The compound was recrystallized from ether - n-hexane to provide 4.8 g of the benzylated compound 2 (66%) as colorless needle crystals. M.p: 77-78 °C. TLC (Rf: 0.6, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.15 (s,3H), 2.51 (s,3H), 3.92 (s,3H), 5.12 (s,2H), 6.34 (s,1H), 7.28-7.50 (m,5H), 11.84 (s,1H) IR (Nujol): 1648, 1577, 803 cm -1 Elementary Analysis (for C 17 H 18 O 4 ) Theory: C,71.31; H,6.34 (%) Found : C,70.99; H,6.32 (%) [Step 2]( 2 → 3 ) A mixture containing the compound 2 (4.8 g, 16.8 mM), Me 2 SO 4 (4.74 ml, 16.8 mM x 3), K 2 CO 3 (11.58 g, 16.8 mM x 5), and 200 ml of acetone were heated under reflux for two hours, and, after cooling, the resultant solid was filtered off. The filtrate was distributed between ethyl acetate and 2 N hydrochloric acid, and the organic phase was washed with water, dried over Na 2 SO 4 , and then concentrated in vacuo to yield the crude product 3 . The product was recrystallized from ether - n-hexane to provide 5.04 g of the compound 3 (100%) as colorless needle crystals. M.p: 56-57°C. TLC (Rf: 0.5, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.17 (s,3H), 2.29 (s,3H), 3.77 (s,3H), 3.90 (s,3H), 5.07 (s,2H), 6.54 (s,1H), 7.24-7.50 (m,5H) IR (Nujol); 1725, 1605, 1580, 760, 703 cm -1 Elementary Analysis (for C 18 H 20 O 4 ) Theory: C,71.98; H,6.71 (%) Found : C,71.97; H,6.90 (%) [Step 3] ( 3 → 4 ) Compound 3 (5.04 g, 16.8 mM) was dissolved in 68.2 ml of DMSO, and KOH (5.64 g, 16.8 mM x 6) which had been dissolved in 13.6 ml of water at 0°C was added to the solution, and then the mixture was stirred overnight at 90 o C. After cooling, the mixture was poured into 2 N hydrochloric acid with ice, and the resultant mixture was extracted with ether. The organic phase was washed with water, and then dried, and concentrated in vacuo to yield the crude product 4 . The product was recrystallized from ether - n-hexane to provide 4.44 g of the compound 4 (93%) as colorless flaky crystals. M.p: 124-125°C. TLC (Rf: 0.3, Developer: chloroform - methanol (9:1)). 1 HNMR (CDCl 3 ): δ 2.20 (s,3H), 2.56 (s,3H), 3.85 (s,3H), 5.12 (s,2H), 6.65 (s,1H), 7.25-7.50 (m,5H) IR (Nujol): 2200-3360, 1685, 1600, 1567, 1170, 1115 cm -1 Elementary Analysis (for C 17 H 18 O 4 ) Theory: C,71.31; H,6.34 (%) Found : C,71.30; H,6.44 (%) [Step 4] ( 4 → 5 ) A mixture containing the compound 4 (4.34 g, 15.2 mM), Pd-C (800 mg), 25 ml of ethyl acetate, and 6 ml of methanol was subjected to hydrogenation by passing hydrogen gas (340 ml, 15.2 mM) through the mixture. After the catalyst was filtered off, the solution was concentrated in vacuo to yield 2.97 g of the colorless crystals 5 (100%). M.p: 153-155°C. TLC (Rf: 0.25, Developer: chloroform methanol (9:1)). 1 HNMR (CD 3 0D): δ 2.08 (s,3H), 2.24 (s,3H), 3.74 (s,3H), 6.44 (s,1H) IR (Nujol): 2200-3440, 3200, 1715, 1610, 1588, 1263, 1155 cm -1 Elementary Analysis (for C 10 H 12 O 4 ) Theory: C,61.22; H,6.17 (%) Found : C,61.02: H,6.22 (%) [Step 5] ( 5 → 6 ) Compound 5 (2.97 g, 15.2 mM) was dissolved in 30 ml of ethyl acetate, and diphenyldiazomethane (5.89 g, 2 mM x 2) was added thereto at room temperature, and the mixture was left to stand overnight. An excess of diphenyldiazomethane was decomposed by adding 2 N hydrochloric acid thereto, and the resultant mixture was extracted with ethyl acetate. The organic phase was washed with water, dried, and then concentrated in vacuo . The residue was subjected to column chromatography (80 g of SiO 2 , eluent: n-hexane - ethyl acetate (19:1) to (1:1)) to yield the benzhydryl ester 6 . The product was recrystallized from ether - n-hexane to yield 3.76 g of 6 as colorless crystals (69%). M.p: 96-97°C. TLC (Rf: 0.25, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.12 (s,6H), 3.54 (s,3H), 4.97 (s,1H), 6.39 (s,1H), 7.15 (s,1H), 7.21-7.50 (m,10H) IR (Nujol): 3410, 1686, 1610, 1590, 1160, 1095 cm -1 Elementary Analysis (for C 23 H 22 O 4 ) Theory: C,76.22; H,6.12 (%) Found : C,76.31; H,6.12 (%) [Step 1] ( 7 → 8 ) Compound 7 (12.7 g, 40.4 mM), which is known in the literature, was hydrolyzed in a procedure similar to that of Step 3 in Preparation 1, to yield 11.7 g of the compound 8 as colorless crystals (97%). M.p. 122-130°C 1 HNMR (CDCl 3 ): δ 2.22 (s,3H), 2.23 (s,3H), 2.33 (s,3H), 3.82 (s,3H), 4.78 (s,2H), 7.28-7.55 (m,5H) IR (KBr): 3650-2250, 3430, 2950, 1687, 1222, 1105, 737, 693 cm -1 Elementary Analysis (for C 18 H 20 O 4 ) Theory: C,71.98; H,6.71 (%) Found : C,72.17; H,6.76 (%) [Step 2] ( 8 → 9 ) Compound 8 (5.0 g, 16.6 mM) was subjected to hydrogenation by a similar procedure to that of Step 4 in Preparation 1, to yield 3.8 g of the compound 9 as colorless crystals (97%). M.p.130-132°C. TLC (Rf: 0.4, Developer: chloroform - methanol (9:1)). 1 HNMR (CDCl 3 ): δ 2.16 (s,3H), 2.19 (s,3H), 2.35 (s,3H), 3.81 (s,3H) IR (KBr): 3660-2100, 3320, 1714, 1562, 1380, 1205 cm -1 Elementary Analysis (for C 11 H 14 O 4 ) Theory: C,62.85; H,6.71 (%) Found : C,62.58; H,6.65 (%) [Step 3] ( 9 → 10 ) Compound 9 (3.36 g, 16.0 mM) was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, to yield 5.76 g of the ester 10 (96%) as colorless pillar crystals. M.p.125-127 °C. TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.06 (s,3H), 2.11 (s,3H), 2.15 (s,3H), 3.52 (s,3H), 4.82 (s,1H), 7.17 (s,1H), 7.22-7.50 (m,10H) IR (Nujol): 3360, 1695, 1585, 1290, 1205 cm -1 Elementary Analysis (for C 24 H 24 O 4 ) Theory: C,76.57; H,6.43 (%) Found : C,76.49; H,6.55 (%) [Step 4] ( 8 + 10 → 11 ) The compound 8 (4.19 g, 13.28 mM x 1.05) was dissolved in 20 ml of CH 2 Cl 2 , and oxalyl chloride (4.25 ml, 13.28 mM x 1.05 x 3.5) was added thereto at room temperature, and the resultant mixture was stirred at room temperature for 30 minutes, and then the mixture was gently warmed under reflux for 30 minutes. After concentration in vacuo , the residue was dissolved in THF, and the solution was again concentrated in vacuo . Alternatively, compound 10 (5.00 g, 13.28 mM) was dissolved in 20 ml of THF, and n-BuLi (1.6 M solution in hexane, 8.30 ml, 13.28 mM) was added gradually to the solution at -78 °C, and then the solution was stirred at the same temperature for 30 minutes. To the reaction mixture was added a solution of the acid chloride 8 obtained above in THF (30 ml) at -78 °C, and the mixture was stirred at the same temperature for 10 minutes. The mixture was allowed to warm slowly to room temperature, and left to stand overnight. The reaction mixture was distributed between ethyl acetate and 1 N hydrochloric acid, and the organic phase was washed with water, and then dried. After the mixture was concentrated in vacuo , the residue was subjected to column chromatography (200 g of SiO 2 , eluent: toluene - ethyl acetate (0%-10%)) to yield 7.32 g of 11 as colorless crystals (84%). M.p: 183-185 °C. TLC (Rf: 0.7, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 2.09 (s,3H), 2.22 (s,3H), 2.25 (s,3H), 2.26 (s,3H), 2.28 (s,3H), 2.37 (s,3H), 3.58 (s,3H), 3.81 (s,3H), 4.80 (s,2H), 7.20 (s,1H), 7.28-7.55 (m,15H) IR (Nujol): 1760, 1723, 1573, 1455, 1156, 740, 697 cm -1 Elementary Analysis (for C 42 H 42 O 7 ) Theory: C,76.57; H,6.43 (%) Found : C,76.83; H,6.55 (%) [Step 5] ( 11 → 12 ) Compound 11 (7.32 g, 11.1 mM) was subjected to hydrogenation by a procedure similar to that of Step 4 in Preparation 1, and the resultant crude compound 12 was recrystallized from toluene - n-hexane to yield 4.31 g of 12 as colorless crystals (96%). M.p. 207-209 °C. TLC (Rf: 0.2, Developer: chloroform - methanol (9:1)). 1 HNMR (DMSO): δ 2.10-2.30 (m,18H), 3.69 (s,3H), 3.71 (s,3H), 8.70-8.93 (brs,1H) IR (KBr): 3650-2260, 3500, 1750, 1576, 1460, 1410, 1170 cm -1 Elementary Analysis (for C 22 H 26 O 7 ) Theory: C,65.66; H,6.51 (%) Found : C,65.50; H,6.58 (%) [Step 6] ( 12 → 13 ) Compound 12 (4.31 g, 10.7 mM) was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, and the resultant compound was recrystallized from ether - n-hexane to yield 5.31 g of 13 (87%) as colorless pillar crystals. M.p. 195-197 °C. TLC (Rf: 0.4, Developer: toluene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 2.08 (s,3H), 2.19-2.24 (m,12H), 2.37 (s,3H), 3.56 (s,3H), 3.80 (s,3H), 4.95 (s,1H), 7.19 (s,1H), 7.28-7.52 (m,10H) IR (Nujol): 3390, 1737, 1706, 1285, 1156, 759, 700 cm -1 Elementary Analysis (for C 35 H 36 O 7 ) Theory: C,73.92; H,6.38 (%) Found : C,73.75; H,6.41 (%) 14 → 16 5-Bromo-2,4-dihydroxy benzoic acid, mono-hydrate 14 (10 g, 39.8 mM) was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, to yield 15 , and the latter compound was dimethoxylated by the procedure of Step 2 in Preparation 1 to yield 16 as crude cystals. The resultant compound was recrystallized from ether - n-hexane to yield 13.6 g of 16 (80%) as colorless pillar crystals. M.p. 124-125 °C. TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 3.94 (s,3H), 3.95 (s,3H), 6.48 (s,1H), 7.07 (s,1H), 7.21-7.48 (m,10H), 8.16 (s,1H) IR (Nujol): 1708, 1600, 1248, 1214, 1029 cm -1 Elementary Analysis (for C 22 H 19 O 4 Br) Theory: C,61.84; H,4.48; Br,18.70 (%) Found : C,61.85; H,4.61; Br,18.43 (%) [Step 1] ( 1 → 17 ) Compound 1 (5.0 g, 25.5 mM), which is known in the literature , was suspended in 50 ml of 1,2-dichloroethane, and 10 ml of a solution of bromine (945 µl, 25.5 mM x 1.2) in 1,2-dichloroethane was added to the suspension, and the mixture was stirred overnight. The mixture was diluted with chloroform, and then the solution was washed with water, 5% aqueous sodium sulfite, and water successively in this order, and then dried. After concentration in vacuo the residue was subjected to column chromatography (90 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1) to (2:1)) to yield 17 . The resultant compound was recrystallized from ether - n-hexane to yield 5.64 g of 17 (80%) of colorless pillar crystals. M.p. 87-88 °C. TLC (Rf: 0.75, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.18 (s,3H), 2.64 (s,3H), 3.95 (s,3H), 6.15 (s,1H), 11.63 (s,1H) IR (Nujol): 3440, 3420, 1655, 1607, 1265 cm -1 Elementary Analysis (for C 10 H 11 O 4 Br) Theory: C,43.66; H,4.03; Br,29.05 (%) Found : C,43.63; H,4.08; Br,29.13 (%) [Step 2] ( 17 → 19 ) Compound 17 (5.72 g, 20.8 mM) was dimethoxylated by the same procedure as Step 2 in Preparation 1 to yield the crude compound 18 , which was directly hydrolyzed by the same procedure of Step 3 in Preparation 1 to yield the crude compound 19 . The compound was recrystallized from petroleum ether to yield 4.69 g of the compound 19 (78%) as colorless needle crystals. M.p. 115-116 °C. TLC (Rf: 0.7, Developer: 1% acetic acid - ethyl acetate). 1 HNMR (CDCl 3 ): δ 2.28 (s,3H), 2.47 (s,3H), 3.82 (s,3H), 3.83 (s,3H) IR (Nujol): 3380-2000, 1690, 1582, 1556, 1303, 1105, 680 cm -1 Elementary Analysis (for C 11 H 13 O 4 Br) Theory: C,45.70; H,4.53; Br,27.64 (%) Found : C,45.81; H,4.62; Br,27.37 (%) [Step 3] ( 19 → 20 ) Compound 19 (1.30 g, 4.50 mM) was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, and the resultant compound was subjected to column chromatography (60 g of SiO 2 , eluent: n-hexane - ethyl acetate (10:0) to (9:1)) to yield 20 as crystals, which were recrystallized from ethyl acetate - hexane to yield 1.87 g of 20 (91%) of colorless pillar crystals. M.p. 136-137 °C. TLC (Rf: 0.6, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.19 (s,3H), 2.24 (s,3H), 3.54 (s,3H), 3.78 (s,3H), 7.12 (s,1H), 7.24-7.46 (m,10H) IR (Nujol): 1725, 1266, 1160, 743, 699 cm -1 2-Hydroxy-3-methyl benzoic acid 21 (20 g, 131 mM) was dissolved in an aqueous sodium hydroxide (32 g of NaOH, 335 ml of water), and 260 ml of a solution of K 2 S 2 O 8 (40 g, 131 mM x 1.31) in water was added thereto at 0 °C. The reaction mixture was stirred at room temperature for four hours, and then left to stand for four days. The addition of dilute sulfuric acid (44 ml of sulfuric acid, 300 ml of water) resulted in the appearance of a brown precipitate. The precipitate was filtered off, and then the filtrate was heated under reflux for 9 hours. After cooling, the solution was partitioned by adding ethyl acetate and NaCl, and the organic phase was passed through a small amount of silica gel (40 g), and the resultant elution was concentrated to yield 22 as a brown crystalline powder. The powder was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, and the resultant compound was subjected to column chromatography (150 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1)) to yield 15.2 mg of 23 (34.8%) as a pale yellow gummy material. 1 HNMR (CDCl 3 ): δ 2.22 (s,3H), 4.55 (brs,1H), 6.90 (d,J=3.2Hz,1H), 7.09 (s,1H), 7.26-7.50 (m,11H), 10.50 (s,1H) Compound 23 (7.6 g, 22.7 mM) was benzylated by a procedure similar to that of Step 1 in Preparation 1, to yield the crude compound 24 . The compound was subjected to column chromatography (150 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1)) to yield 8.1 g of 24 as a pale yellow oil (84.4%). Subsequently, the resultant compound was methoxylated by the procedure of Step 2 in Preparation 1 to yield the crude compound 25 . The compound was subjected to column chromatography (150 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1) to (4:1)) to yield 7.77 g of 25 as a pale yellow oil (92%). TLC (Rf: 0.4, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.29 (s,3H), 3.66 (s,3H), 5.03 (s,2H), 6.99 (d,J=3.0Hz,1H), 7.12 (s,1H), 7.20-7.50 (m,16H) [Step 1] ( 25 → 26 ) Compound 25 (7.53 g, 17.2 mM) was subject to hydrogenation by a procedure similar to that of Step 4 in Preparation 1, to yield 26 as crude crystals. The compound was recrystallized from ether - petroleum ether to yield 2.83 g of 26 (90%) of colorless prism crystals. M.p. 142-144 °C. TLC (Rf: 0.5, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 2.17 (s,3H), 3.64 (s,3H), 6.77 (d,J=3.0Hz,1H), 6.87 (d,J=3.0Hz,1H), 9.34 (brs,1H) IR (Nujol): 3320, 1679, 1606 cm -1 Elementary Analysis (for C 9 H 10 O 4 ) Theory: C,59.34; H,5.53 (%) Found : C,59.32; H,5.56 (%) [Step 2] ( 26 → 27 ) Compound 26 (2.62 g, 14.4 mM) was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, to yield the crude compound 27 . The compound was purified by column chromatography (150 g of SiO 2 , eluent: n-hexane - ethyl acetate (19:1) to (2:1)), and the resultant compound was crystallized from ether - petroleum ether to yield 3.45 g of 27 (69%) as colorless prism crystals. M.p. 101-103 °C. TLC (Rf: 0.4, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 2.27 (s,3H), 3.66 (s,3H), 4.92 (s,1H), 6.85 (d,J=3Hz,1H), 7.12 (s,1H), 7.18 (d,J=3Hz,1H), 7.21-7.50 (m,10H) IR (Nujol): 3430, 1689 cm -1 Elementary Analysis (for C 22 H 20 O 4 ) Theory: C,75.84; H,5.79 (%) Found : C,75.89; H,5.81 (%) Synthesis of 78 To compound 1 (1.00 g, 51.0 mM), which is known in the literature , was added ice-cooled conc. sulfuric acid (30 ml), and the mixture was left to stand overnight. The mixture was poured onto ice, and extracted with ether. The ether phase was extracted with aqueous saturated sodium bicarbonate, and the water phase was acidified with 2 N hydrochloric acid and extracted again with ether. The extract was washed with water, dried, and then concentrated in vacuo , to yield 7.63 g of 78 as colorless crystals (82%). M.p. 190-192 °C. TLC (Rf: 0.2, Developer: chloroform - methanol (9:1)). 1 HNMR (DMSO): δ 1.93 (s,3H), 2.40 (s,3H), 3.36 (brs,1H), 6.26 (s,1H), 10.05 (s,1H), 12.90 (brs,1H) IR (KBr): 3660-2080, 3410, 1639, 1458, 1262, 1175, 1091 cm -1 Elementary Analysis (for C 9 H 10 O 4 ) Theory: C,59.34; H,5.53 (%) Found : C,59.25; H,5.56 (%) Synthesis of 64 Compound 63 (1.00 g, 1.76 mM) was benzhydrylated by a procedure similar to that of Step 5 in Preparation 1, to yield the crude compound 64 . The compound was crystallized from ethyl acetate - n-hexane to yield 765 mg of 64 as colorless crystals (59%). M.p. 198-200 °C. TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.02-2.28 (m,18H), 2.40 (s,3H), 2.65 (s,3H), 3.57 (s,3H), 3.83 (s,3H), 5.44 (s,1H), 6.32 (s,1H), 7.20 (s,1H), 7.28-7.50 (m,10H), 11.86 (s,1H) Example 1 4-Carboxyphenyl (5'-carboxy-2',4'-dimethoxyphenyl) ether [Step 1] ( 28 → 29 ) 4-Hydroxy benzoic acid 28 (2.76 g, 20 mM) was benzhydrylated in a procedure similar to that of Step 5 in Preparation 1, to yield the crude compound 29 . The compound was recrystallized from methylene chloride - toluene to yield 4.47 g of 29 (74%). M.p. 135-137 °C. TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 5.63 (s,1H), 6.80-6.90 (m,A 2 B 2 A-Part,2H), 7.08 (s,1H), 7.21-7.48 (m,10H), 7.98-8.12 (m,A 2 B 2 B-Part,2H) [Step 2] ( 29 + 16 → 30 ) Compound 29 (1.61 g, 5.29 mM) was dissolved in 15 ml of DMF, and to the solution was added NaH (212 mM, 5.29 mM) in a stream of argon at 0 °C. After the mixture was stirred at room temperature for 30 minutes, a complex of copper bromide dimethyl sulfide (3.26 g, 5.29 mM x 3) was added thereto, and the mixture was stirred for a while. Compound 16 (2.26 g, 5.29 mM) was added thereto, and then the mixture was stirred at 160 °C for about 20 hours. After cooling, the resultant mixture was distributed between ethyl acetate and 2 N hydrochloric acid, and the organic phase was washed with hydrochloric acid three times, water, and then dried, concentrated in vacuo , and the residue was subjected to column chromatography (70 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1) to (1:1)), to yield 180 mg of 30 as an oil (5%). TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 3.84 (s,3H), 3.97 (s,3H), 6.59 (s,1H), 6.85-6.94 (m,A 2 B 2 A-part,2H), 7.06 (s,1H), 7.09 (s,1H), 7.18-7.48 (m,20H), 7.77 (s,1H), 8.03-8.12 (m,A 2 B 2 B-part,2H) [Step 3] ( 30 → 31 ) A mixture containing 30 (180 mg, 277 mM), trifluoroacetic acid (1 ml), and anisole (0.5 ml) was stirred at room temperature for 30 minutes, and after concentration in vacuo , the residue was crystallized by adding ether thereto, to yield 62 mg of 31 (70%). M.p. 237-239 °C TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 3.86 (s,3H), 3.99 (s,3H), 6.80-6.90 (m,A 2 B 2 A-part,2H), 6.85 (s,1H), 7.64 (s,1H), 7.90-8.00 (m,A 2 B 2 B-part,2H) IR (KBr): 3680-2320, 3300, 1725, 1675, 1618, 1230, 1023 cm -1 Elementary Analysis (for C 16 H 14 O 7 ) Theory: C,60.38; H,4.43 (%) Found : C,60.12; H,4.70 (%) Example 2 4-Carboxy-3-methoxy-2,5-dimethylphenyl (5'-carboxy-2',4'-dimethoxyphenyl) ether [Step 1] ( 6 + 16 → 32 ) Compound 6 (2.0 g, 5.52 mM), and 16 (2.36 g, 5.52 mM) were treated in a procedure similar to that of Step 2 in Example 1, to yield the crude compound 32 . The compound was subjected to column chromatography (90 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1) to (1:1)), to yield 670 mg of 32 as an oil (17%). TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.04 (s,3H), 2.24 (s,3H), 3.58 (s,3H), 3.86 (s,3H), 3.96 (s,3H), 6.16 (s,1H), 6.58 (s,1H), 7.05 (s,1H), 7.16 (s,1H), 7.18-7.58 (m,20H), 7.63 (s,1H) [Step 2] ( 32 → 33 ) Compound 32 (670 mg, 945 µM) was treated in a similar procedure to that of Step 3 in Expample 1, to yield 288 mg of 33 as colorless crystals (81%). M.p. 199-200 °C. TLC (Rf: 0.5, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 2.10 (s,3H), 2.14 (s,3H), 3.73 (s,3H), 3.87 (s,3H), 3.89 (s,3H), 6.17 (s,1H), 6.86 (s,1H), 7.32 (s,1H) IR (KBr): 3680-2730, 3430, 3260, 1731, 1688, 1611, 1512, 1440, 1423 cm -1 Elementary Analysis (for C 19 H 20 O 8 ·0.75H 2 O) Theory: C,58.53; H,5.56 Found : C,58.51; H,5.25 Example 3 3-Carboxy-4-methoxy-5-methylphenyl-(5,-carboxy-2',4'-dimethoxyphenyl) ether [Step 1] ( 27 + 16 → 34 ) Compound 27 (1.0 g, 2.87 mM), and 16 (1.23 g, 2.87 mM) were treated in a procedure similar to that of Step 2 in Expample 1, to yield the crude compound 34 . The compound was subjected to column chromatography (80 g of SiO 2 , eluent: n-hexane - ethyl acetate (4:1) to (2:1)), to yield 400 mg of 34 as an oil (20%). TLC (Rf: 0.1, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.26 (s,3H), 3.67 (s,3H), 3.86 (s,3H), 3.96 (s,3H), 6.58 (s,1H), 6.87 (d,J=4Hz,1H), 7.05 (s,1H), 7.07 (s,1H), 7.10-7.54 (m,11H), 7.70 (s,1H) [Step 2] ( 34 → 35 ) Compound 34 (320 mg, 461 µM) was treated in a procedure similar to that of Step 3 in Expample 1, to yield 112 mg of 35 as pale yellow crystals (67%). TLC (Rf: 0.3, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 2.22 (s,3H), 3.69 (s,3H), 3.86 (s,3H), 3.90 (s,3H), 6.82 (d,J=3.0Hz,1H), 6.87 (s,1H), 6.95 (d,J=3.0Hz,1H), 7.39 (s,1H) IR (KBr): 3680-2320, 3440, 2960, 1695, 1616, 1215, 1120 cm -1 Elementary Analysis (for C 18 H 18 O 8 ·0.3H 2 O) Theory: C,58.79; H,5.10 (%) Found : C,58.86; H,5.09 (%) Example 4 Bis(3-carboxy-4,6-dimethoxy-2,5-dimethylphenyl) methane [Step 1] Synthesis of acetate 39 The aldehyde 36 (3.70 g, 16.5 mM), which is described in the literature , was methoxylated by a similar procedure to that of Step 2 in Preparation 1, to yield 6.8 g of 37 as a red oil. The compound was dissolved in 30 ml of isopropyl alcohol, and to the solution was added NaBH 4 (0.94 g, 16.5 mM x 1.5), and the reaction mixture was stirred, and then it was distributed between ethyl acetate and water. The organic phase was washed with 2 N hydrochloric acid, followed by water, dried, and then concentrated in vacuo . The resultant residue was subjected to column chromatography (70 g of SiO 2 , eluent: n-hexane - ethyl acetate (4:1) to (1:1)), to yield 3.18 g of the alcohol 38 (76% based on 1 ) as a colorless oil. TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (4:1)). Thereafter, the oil was dissolved in 20 ml of CH 2 Cl 2 , and to the solution was added 6 ml of acetic anhydride, 10 ml of pyridine, and 50 mg of dimethylaminopyridine, and then the mixture was stirred at room temperature for two hours. The reaction mixture was distributed between ethyl acetate and 2 N hydrochloric acid, and the organic phase was washed with water, and dried, then concentrated in vacuo to yield the crude compound 39 . The compound was recrystallized from ether - n-hexane to yield 3.35 g of the acetate 39 (91%) as colorless pillar-crystals. M.p. 69-70 °C. TLC (Rf: 0.4, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.07 (s,3H), 2.22 (s,3H), 2.24 (s,3H), 3.74 (s,3H), 3.78 (s,3H), 3.93 (s,3H), 5.18 (s,2H) [Step 2] Synthesis of 40 Compound 1 (1.45 g, 6.74 mM x 1.1), which is known in the literature and the acetate 39 (2.00 g, 6.74 mM) were dissolved in 25 ml of toluene, and to the solution was added BF 3 ·OEt 2 (0.83 ml, 6.74 mM), and the mixture was stirred at room temperature for 15 minutes. To the reaction mixture was added ethyl acetate, then the mixture was washed with water, dried, and then concentrated in vacuo . The residue was subjected to column chromatography (SiO 2 ; Merck, Lobar B, eluent: n-hexane - ethyl acetate), to yield 2.91 g of 40 (100%) as colorless prism crystals. M.p. 169-170 °C. TLC (Rf: 0.3, Developer: n-hexane - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 2.07 (s,6H), 2.25 (s,3H), 2.55 (s,3H), 3.75 (s,6H), 3.88 (s,3H), 3.92 (s,3H), 3.95 (s,2H), 7.20 (s,1H), 11.38 (s,1H). IR (Nujol): 3285, 1743, 1644 cm -1 Elementary Analysis (for C 23 H 28 O 8 ) Theory: C,63.87; H,6.54 (%) Found : C,63.65; H,6.57 (%) [Step 3] Synthesis of 41 Compound 40 (2.91 g, 7.4 mM) was methoxylated in a procedure similar to that of Step 2 in Preparation 1, and the crude residue was subjected to column chromatography (60 g of SiO 2 , eluent: toluene - ethyl acetate (9:1) to (4:1)), to yield 2.97 g of 41 (96%) as colorless pillar crystals. M.p. 271-220 °C. TLC (Rf: 0.5, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 2.03 (s,6H), 2.20 (s,6H), 3.48 (s,6H), 3.74 (s,6H), 3.88 (s,6H), 4.03 (s,2H) IR (Nujol): 1730, 1588, 1572, 1104 cm -1 Elementary Analysis (for C 25 H 32 O 8 ) Theory: C,65.19; H,7.02 (%) Found : C,65.14; H,7.04 (%) [Step 4] ( 41 → 42 ) Compound 41 (600 mg, 130 mM) was hydrolyzed in a procedure similar to that of Step 3 in Preparation 1, to yield 559 mg of 42 as colorless crystals (99%). M.p. 265-267 °C. TLC (Rf: 0.7, Developer: ethyl acetate - acetic acid - water (18:1:1)). 1 HNMR (DMSO): δ 1.96 (s,6H), 2.12 (s,6H), 3.48 (s,6H), 3.66 (s,6H), 3.98 (s,2H) IR (KBr): 3670-2400, 3430, 2940, 1710, 1217, 1105 cm -1 Elementary Analysis (for C 23 H 28 O 8 ·0.4H 2 O) Theory: C,62.83; H,6.60 (%) Found : C,62.88; H,6.63 (%) Example 5 Bis[5-[4'-(4''-carboxy-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl)-3'-methoxy-2',5',6',-trimethylphenoxycarbonyl]-2,4-dimethoxy-3,6-dimethylphenyl] methane [Step 1] Synthesis of 43 Compound 42 (100 mg, 231 µM) and 13 (263 mg, 231 µM x 2) were esterified in a procedure similar to that of Step 4 in Preparation 2, to yield the crude compound 43 . The compound was subjected to column chromatography (15 g of SiO 2 , eluent: n-hexane - ethyl acetate (4:1) to (2:1)), to yield 219 mg of 43 as colorless crystals (62%). M.p. 245-247 °C. TLC (Rf: 0.7, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 2.08 (s,6H), 2.20 (s,6H), 2.25 (s,12H), 2.30 (s,18H), 2.39 (s,6H), 3.57 (s,6H), 3.60 (s,6H), 3.83 (s,12H), 4.17 (s,2H), 7.19 (s,2H), 7.22-7.50 (m,20H) IR (Nujol): 1745, 1150, 1097, 1074 cm -1 Elementary Analysis (for C 93 H 96 O 20 ) Theory: C,72.83; H,6.31 (%) Found : C,72.74; H,6.33 (%) [Step 2] Synthesis of 44 Compound 43 (186 mg, 121 µM) was deprotected in a procedure similar to that of Step 3 in Example 1, to yield 140 mg of 44 as a colorless powder (96%). TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 2.12-2.40 (m,48H), 3.54 (s,6H), 3.73 (s,6H), 3.77 (s,12H), 4.14 (s,2H) IR (KBr): 3680-2400, 3450, 2940, 1743, 1697, 1140 cm -1 Elementary Analysis (for C 67 H 76 O 20 ) Theory: C,66.99; H,6.38 (%) Found : C,66.73; H,6.31 (%) Example 6-11 The following compounds were synthesized as shown above. Example Compound Starting Material Ratio (COOH:OH) yield COOH OH 6 46 42 10 1:1 17% 7 48 42 13 1:1 20% 8 50 42 10 1:2 70% 9 52 isophthalic acid 13 1:2 60% 10 54 chelidonic acid 13 1:2 27% 11 56 35 13 1:2 42% Physicochemical data Compound 46 ; Colorless crystals. M.p. 141-143 °C. TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (CD 3 OD): δ 1.99 (s,3H), 2.10 (s,3H), 2.16-2.28 (m,15H), 3.54 (s,3H), 3.62 (s,3H), 3.77 (s,3H), 3.78 (s,3H), 3.80 (s,3H), 4.16 (s,2H) IR (KBr): 3420, 2930, 1738, 1569, 1150, 1095 cm -1 Compound 48 ; Colorless powder. TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (CD 3 OD): δ 2.00 (s,3H), 2.11 (s,3H), 2.18-2.34 (m,21H), 2.41 (s,3H), 3.57 (s,3H), 3.63 (s,3H), 3.77 (s,3H), 3.82 (s,3H), 3.83 (s,3H), 4.18 (s,2H) IR (KBr): 3440, 2950, 1745, 1703, 1570, 1460, 1145, 1095, 1075 cm -1 Compound 50 ; Colorless crystals. M.p. 267-269 °C. TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 2.04-2.28 (m,30H), 3.52 (s,6H), 3.70 (s,6H), 3.74 (s,6H), 4.11 (s,2H) IR (KBr): 3440, 2940, 1740, 1700, 1460, 1155 cm -1 Elementary Analysis (for C 45 H 52 O 14 ) Theory: C,66.16; H,6.42 (%) Found : C,65.97; H,6.48 (%) Compound 52 ; Colorless crystals. M.p. 267-269 °C. TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 2.08-2.26 (m,30H), 2.37 (s,6H), 3.73 (s,6H), 3.79 (s,6H), 7.98 (t,J=9Hz,1H), 8.66 (dd,J=9Hz,J=1Hz,2H), 8.93 (m,1H) IR (KBr): 3430, 2940, 1745, 1700, 1222, 1160 cm -1 Elementary Analysis (for C 52 H 54 O 16 ·H 2 O) Theory: C,65.54; H,5.92 (%) Found : C,65.44; H,5.84 (%) Compound 54 ; Colorless plate crystals. M.p. 294-296 °C. TLC (Rf: 0.3, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (CDCl 3 ): δ 2.16 (s,6H), 2.21 (s,6H), 2.26 (s,6H), 2.29 (s,6H), 2.36 (s,6H), 2.42 (s,6H), 3.84 (s,6H), 3.87 (s,6H), 7.52 (s,2H) Elementary Analysis (for C 51 H 52 O 18 ) Theory: C,64.27; H,5.51 (%) Found : C,63.88; H,5.57 (%) Compound 56 ; Colorless powder (hygroscopic). TLC (Rf: 0.3, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 1.90 (s,3H), 1.98-2.90 (m,27H), 2.28-2.37 (m,9H), 3.68-3.81 (m,15H), 3.96 (s,3H), 4.00 (s,3H), 7.03 (s,1H), 7.18 (d,J=2.8Hz,1H), 7.28 (d,J=2.8Hz,1H), 7.80 (s,1H) IR (KBr): 3430, 2930, 1745, 1575, 1462, 1150 cm -1 Elementary Analysis (for C 62 H 66 O 20 ·3.5H 2 O) Theory: C,62.36; H,6.16 (%) Found : C,62.31; H,5.92 (%) Example 12 n-Butyl 3-[3'-(4''-carboxy-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl)-4'-methoxyphenyl]-thio-6-methoxybenzoate Synthesis of 59 Compound 57 (50 mg, 150 µM) and 10 (56 mg, 150 µM) were treated in a procedure similar to that of Step 4 in Preparation 2, to yield the half-ester. The compound was reacted with n-butyl lithium (1.6 N solution in hexane, 93 µl, 150 µM), and the reaction mixture was worked up in a conventional manner to yield the crude compound 58 . The compound was subjected to column chromatography (3 g of SiO 2 , eluent: toluene - ethyl acetate (1:0) to (4:1)), to yield 58 as a colorless oil. The oil was deprotected in a procedure similar to that of Step 3 in Example 1 to yield 14 mg of 59 (16%) as a colorless oil. TLC (Rf: 0.5, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (CDCl 3 ): δ 0.95 (t,J=7.2Hz,3H), 1.30-1.53 (m,2H), 1.59-1.80 (m,2H), 2.08 (s,3H), 2.09 (s,3H), 2.30 (s,3H), 3.80 (s,3H), 3.89 (s,3H), 3.92 (s,3H), 4.27 (t,J=7Hz,2H), 6.90-7.05 (m,2H), 7.40-7.54 (m,2H), 7.82 (d,J=1.2Hz,1H), 8.01 (J=1.2Hz,1H) Example 13 Bis[5-[4'-(4''-pivaloyloxymethyloxycarbonyl-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl)-3'-methoxy-2',5',6'-trimethylphenoxycarbonyl]-2,4-dimethoxy-3,6-dimethylphenyl] methane Synthesis of 60 Compound 44 (100 mg, 83.2 µM) was dissolved in acetone (1 ml), and to the solution was added pivaloyloxymethyl iodide (tBuCOOCH 2 I: 48 mg, 83.2 µM x 2.4) dissolved in 3 ml of acetone and then anhydrous K 2 CO 3 (40 mg, 83.2 µM x 3.5) was added thereto, and the resultant mixture was stirred at room temperature for two and a half days. The reaction mixture was worked up in a conventional manner, and then it was subjected to column chromatography (10 g of SiO 2 , eluent: toluene - ethyl acetate (19:1) to (9:1)), and the residue was crystallized from ether - n-hexane, to yield 103 mg of 60 as colorless crystals (87%). M.p. 211-213 °C. TLC (Rf: 0.3, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 1.28 (s,18H), 2.20-2.37 (m,42H), 2.41 (s,6H), 3.62 (s,6H), 3.74-3.88 (m,18H), 4.27 (s,2H), 6.01 (s,4H) IR (Nujol): 1751, 1570, 1150, 983 cm -1 Elementary Analysis (for C 79 H 96 O 24 ·H 2 O) Theory: C,65.55; H,6.82 (%) Found : C,65.58; H,6.63 (%) Example 14 Bis[5-[4'-(4''-carboxy-3''-hydroxy-2'',5'',6''-trimethylphenoxycarbonyl)-3'-hydroxy-2',5',6'-trimethylphenoxycarbonyl]-2,4-dihydroxy-3,6-dimethylphenyl] methane [Step 1] 43 → 61 A mixture containing the compound 43 (500 mg, 326 µM), BBr 3 (371 µl, 326 µM x 12), and CH 2 Cl 2 (15 ml) was stirred at room temperature for 6 hours, and water was added thereto, and then the resultant mixture was distributed between ethyl acetate and brine. The organic phase was washed with brine three times, dried, and concentrated in vacuo . The residue was dissolved in ethyl acetate (6 ml), and the solution was subjected to benzhydrylation in a procedure similar to that of Step 5 in Preparation 1, to yield the crude compound 61 . The compound was subjected to column chromatography (Lober (Merck: B), eluent: toluene - ethyl acetate (1:0) to (0:1)), and the residure was powdered in ethyl - n-hexane to yield 162 mg of 61 as a colorless powder (35%). TLC (Rf: 0.5, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 2.00-2.20 (m,30H), 2.55 (s,6H), 2.67 (s,6H), 2.74 (s,6H), 4.17 (s,2H), 6.31 (s,2H), 7.20 (s,2H), 7.28-7.48 (m,20H), 11.04 (s,2H), 11.33 (s,2H), 11.56 (s,2H) [Step 2] 61 → 62 Compound 61 (61 mg, 106 µM) was subjected to hydrogenation in a procedure similar to that of Step 4 in Preparation 1, to yield 62 . The compound was crystallized from ether to yield 49 mg of 62 as colorless crystals (43%). M.p. (dec.); 162-220 °C. TLC (Rf: 0.2, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (DMSO): δ 1.96-2.40 (m,48H), 4.09 (s,2H), 9.43 (s,2H), 9.90 (s,2H) IR(Nujol): 3700-2560, 1670, 1455, 1155 cm -1 . Example 15-17 [Step 1] Synthesis of the compound 67 Diazomethane gas was generated by stirring a solution of N-methyl nitrosourea (10 g, 20.2 mM x 4.8) in ether and an aqueous solution of potassium hydroxide, and the gas was trapped in ice-cooled ether, to make a solution of diazomethane in ether. Then, the ether solution was added to a methanol solution of N-t-Boc-O-benzyl-L-tyrosine 65 (7.5 g, 20.2 mM), until the solution developed a yellow color. Acetic acid was added thereto until the solution became colorless in order to decompose excess diazomethane. The material was concentrated in vacuo to yield 66 . The compound was dissolved in methanol, and it was subjected to hydrogenation in a procedure similar to that of Step 4 in Preparation 1, to yield the crude compound 67 . The compound was recrystallized from ether - n-hexane to yield 5.88 g of 67 as colorless crystals (99%). M.p. 100-101 °C. TLC (Rf: 0.3, Developer: benzene - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 1.42 (s,9H), 2.94-3.06 (m,2H), 3.72 (s,3H), 4.26-4.61 (m,1H), 4.92-5.06 (m,1H), 5.42 (s,1H), 6.74 (d,J=8.2Hz,2H), 6.98 (d,J=8.2Hz,2H) IR (Nujol): 3399, 1716, 1690, 1519 cm -1 [α] 25.0 D = +49.1 ± 0.9° (CHCl 3 , C=1.008%) Elementary Analysis (for C 15 H 21 NO 5 ·0.4H 2 O) Theory: C,59.55; H,7.26; N,4.63 (%) Found : C,59.55; H,6.88; N,4.71 (%) [Step 2] 20 + 67 → 68 Compound 20 (2.56 g, 5.61 mM) and 67 (1.66 g, 5.61 mM) were treated in a procedure similar to that of Step 2 in Example 1, to yield the crude compound 68 . The compound was subjected to column chromatography (75 g of SiO 2 , eluent: n-hexane - ethyl acetate (9:1) to (4:1)), to yield 226 mg of 68 (6%) as a colorless foam. TLC (Rf: 0.2, Developer: n-hexane - ethyl acetate (2:1)). 1 HNMR (CDCl 3 ): δ 1.41 (s,9H), 1.95 (s,3H), 2.18 (s,3H), 2.94-3.04 (m,2H), 3.58 (s,3H), 3.70 (s,3H), 3.71 (s,3H), 4.24-4.62 (m,1H), 4.89-5.01 (m,1H), 6.72 (d,J=8.4Hz,2H), 7.00 (d,J=8.4Hz,2H), 7.17 (s,1H), 7.26-7.48 (m,10H) IR (Nujol): 3370, 1720, 1169 cm -1 [α] 24.0 D =+21.4 ± 0.6° (CHCl 3 , C=1.002%) Elementary Analysis (for C 39 H 43 NO 9 ) Theory: C,69.94; H,6.47; N,2.09 (%) Found : C,69.85; H,6.44; N,2.17 (%) [Step 3] 68 → 69 Compound 68 (198 mg, 296 µM) was subjected to hydrogenation in a procedure similar to that of Step 4 in Preparation 1, to yield 149 mg of 69 (100%) as colorless foam. TLC (Rf: 0.1, Developer: benzene - ethyl acetate (4:1)). 1 HNMR (CDCl 3 ): δ 1.41 (s,9H), 2.22 (s,3H), 2.23 (s,3H), 2.94-3.09 (m,2H), 3.71 (s,3H), 3.76 (s,3H), 3.86 (s,3H), 4.48-4.64 (m,1H), 4.94-5.06 (m,1H), 6.72 (d,J=8.6Hz,2H), 7.02 (d,J=8.6Hz,2H) [Step 4] 64 + 69 → 70 + 71 Compound 64 (434 mg, 296 µM x 2) and 69 (149 mg, 296 µM) were treated in a procedure similar to that of Step 4 in Preparation 2, to yield a mixture comprising 70 and 71 . The mixture was subjected to column chromatography (15 g of SiO 2 , eluent: toluene - ethyl acetate (2%) to (28%)), to yield 70 (145 mg, 40%) and 71 (118 mg, 33%). Compound 70 : Colorless glassy material. TLC (Rf: 0.5, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 1.42 (s,9H), 2.05-2.45 (m,27H), 2.76 (s,3H), 3.57 (s,3H), 3.72 (s,3H), 3.78 (s,3H), 3.84 (s,3H), 3.91 (s,3H), 4.52-4.64 (m,1H), 4.93-5.05 (m,1H), 6.70 (s,1H), 6.78 (d,J=8.6Hz,2H), 7.05 (d,J=8.6Hz,2H), 7.20 (s,1H), 7.25-7.50 (m,10H), 11.81 (s,1H) Compound 71 : Colorless glassy material. TLC (Rf: 0.1, Developer: benzene - ethyl acetate (9:1)). 1 HNMR (CDCl 3 ): δ 1.90-2.38 (m,27H), 2.50 (s,3H), 2.88-3.01 (m,2H), 3.57 (s,3H), 3.65 (s,3H), 3.73 (s,3H), 3.74 (s,3H), 3.86 (s,3H), 4.46-4.58 (m,1H), 4.90-5.02 (m,1H), 5.71 (s,1H), 6.61 (d,J=8.4Hz,2H), 6.70 (s,1H), 6.98 (d,J=8.4Hz,2H), 7.20 (s,1H), 7.28-7.50 (m,10H) Example 18 [5-[4'-[4''-(4'''-Carboxy-3'''-methoxy-2''',5''',6'''-trimethylphenoxycarbony)-3''-methoxy-2'',5'',6''-trimethylphenoxycarbony]-3'-hydroxy-2',5'-dimethylphenoxycarbony]-2,4-dimethoxy-3,6-dimethylphenyl] [4''''-(2'''''-amino-2'''''-carboxyethyl)phenyl] ether trifluoroacetate [Step 1] 70 → 73 Compound 70 (128 mg, 105 µM) was hydrolyzed in a procedure similar to that of Step 3 in Preparation 1, to yield 122 mg of 72 (96%) as a colorless oil. TLC (Rf: 0.4, Developer: ethyl acetate - acetic acid (1%)). The compound was subjected to hydrogenation in a procedure similar to that of Step 4 in Preparation 1, to yield 105 mg of 73 (100%) as a colorless powder. TLC (Rf: 0.3, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (CDCl 3 ): δ 2.10-2.46 (m,27H), 2.76 (s,3H), 3.03-3.08 (m,2H), 3.74-3.92 (m,12H), 4.52-4.68 (m,1H), 4.92-5.02 (m,1H), 6.70 (s,1H), 6.80 (d,J=8.6Hz,2H), 7.13 (d,J=8.6Hz,2H), 11.80 (s,1H) [Step 2] 73 → 74 A mixture containing 73 (109 mg, 105 µM), trifluoroacetic acid (1.2 ml), and methylene chloride (2 ml) was stirred at room temperature for two hours, and the resultant mixture was concentrated directly in vacuo . The residue was dissolved in ethyl acetate, and the solution was again concentrated in vacuo . The residue was powdered in ethyl acetate - ether to yield 64 mg of 74 (58%) as a colorless powder. 1 HNMR (DMSO): δ 2.10-2.58 (m,30H), 2.72-2.92 (m,2H), 3.73 (m,6H), 3.78 (s,3H), 3.84 (s,3H), 6.74 (d,J=8.6Hz,2H), 6.80 (s,1H), 7.22 (d,J=8.6Hz,2H) IR (Nujol): 3680-2280, 1750, 1700, 1665, 1610, 1160, 1140, 1080 cm -1 [α] 26.0 D =-12.1 ± 0.8 ° (DMSO, C=0.644%) Elementary Analysis (for C 51 H 55 NO 16 ·0.2CF 3 COOH) Theory: C,64.26; H,5.79; N,1.49; F,1.19 (%) Found : C,64.18; H,5.89; N,1.49; F,0.95 (%) Example 19 [5-[6'-[4''-(4'''-Carboxy-3'''-methoxy-2''',5''',6'''-trimethylphenoxycarbony)-3''-methoxy-2'',5'',6''-trimethylphenoxycarbony]-3'-hydroxy-2',5'-dimethylphenoxycarbony]-2,4-dimethoxy-3,6-dimethylphenyl] [4''''-(2'''''-amino-2'''''-carboxyethyl)phenyl] ether trifluoroacetate [Step 1] 71 → 75 Compound 71 (110 mg, 90.3 µM) was hydrolyzed in a procedure similar to that of Step 3 in Preparation 1, to yield 100 mg of 75 (92%) as a colorless oil. TLC (Rf: 0.5, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (CDCl 3 ): δ 1.90-2.50 (m,30H), 2.78-3.02 (m,2H), 3.56 (s,3H), 3.73 (s,3H), 3.74 (s,3H), 3.87 (s,3H), 4.40-4.54 (m,1H), 4.90-5.00 (m,1H), 6.56 (d,J=8.6Hz,2H), 6.68 (s,1H), 7.00 (d,J=8.6Hz,2H), 7.21 (s,1H), 7.28-7.50 (m,10H) [Step 2] 75 → 76 Compound 75 (100 mg, 83.0 µM) was subjected to hydrogenation in a procedure similar to that of Step 4 in Preparation 1, to yield 70 mg of 76 (81%) as a colorless powder. TLC (Rf: 0.3, Developer: ethyl acetate - acetic acid (1%)). 1 HNMR (d 6 -acetone): δ 1.33 (s,9H), 1.92-2.05 (m,9H), 2.18-2.40 (m,18H), 2.49 (s,3H), 2.97-3.17 (m,2H), 3.75 (s,3H), 3.78 (s,3H), 3.81 (s,3H), 3.93 (s,3H), 4.24-4.46 (m,1H), 5.91-6.02 (m,1H), 6.63 (d,J=8.6Hz,2H), 6.88 (s,1H), 7.18 (d,J=8.6Hz,2H), 7.25 (s,1H) IR (Nujol): 3680-2260, 3340, 1730, 1158 cm -1 [Step 3] 76 → 77 Compound 76 (70 mg, 67.4 µM) was deprotected in a procedure similar to that of Step 2 in Example 18, to yield 52 mg of 77 (73%) as a colorless and hygroscopic powder. (Rf: 0.5, Developer: ethyl acetate - acetic acid - water (8:1:1)). IR (Nujol): 3690-2400, 3400, 1735, 1276, 1158 cm -1 [α] 26.0 D =-9.3 ± 1.0° (DMSO, C=0.515%) Elementary Analysis (for (C 51 H 55 NO 16 ·0.25CF 3 COOH·3.5H 2 O) Theory: C,60.77; H,5.99; N,1.34; F,1.36 (%) Found : C,60.85; H,5.80; N,1.55; F,1.42 (%) Example 20 Bis[3-[4'-[(4''-carboxy-3''-methoxy-2'',5'',6''-trimethyl)phenoxycarbony]-3'-methoxy-2',5',6'-trimethyl]-phenoxycarbony-4-methoxyphenyl] sulfide [Step 1] 5,5'-Thio-disalicylic acid (6.62g, 0.022 mol) was dissolved in 300 ml of acetone, and 11.0 g of MeSO 4 (0.087 mol) and 15.2 g of K 2 CO 3 (0.11 mol) were added thereto, and then the mixture was heated under reflux with stirring for seven hours. After cooling, 400 ml of ice-water was added to the mixture. Then, the resultant mixture was extracted with chloroform (200 ml x 2), and the organic phase was dried (Na 2 SO 4 ), and then evaporated to dryness in vacuo , to yield 8.22 g of the residue as an oil. The oil (8.22 g), which was the crude dimethyl ester, was dissolved in 300 ml of methanol, and 50 ml of 1 M KOH solution in water was added thereto, and then the mixture was heated under reflux for three hours. The mixture was cooled to room temperature, and then the mixture was neutralized by adding 3.3 g of acetic acid thereto. The resultant mixuture was evaporated to dryness in vacuo , and the residue was distributed between chloroform and water. The organic phase was washed with water, dried (Na 2 SO 4 ), and then concentrated in vacuo to yield 7.7 g of the solid. The solid was washed with a small amount of ether to yield 5.78 g of 102a (76%). M.p. 158-159 °C (chlorform - n-hexane). Elementary Analysis (for C 16 H 14 O 6 S) Theory (%): C;57.34, H;4.15, S;9.39 Found (%) : C;57.47, H;4.22, S;9.59 [Step 2] The compound 102a (58.7 mg, 0.18 mmol) obtained above was dissolved in 2 ml of anhydrous methylene chloride, and to the solution was added 0.16 ml of oxalyl chloride (1.8 mmol) dropwise at room temperature, followed addition of two drops of a 10% solution of dimethylformamide in methylene chloride. The mixture was stirred at room temperature for one hour, and then at 40-50°C (temperature of an oil bath) for an additional hour. The reaction mixture was evaporated to dryness in vacuo , and the residue was dissolved in 4 ml of anhydrous tetrahydrofuran, and the solution was evaporated to dryness in vacuo . The remaining excess of the reagents was removed by repeating the procedure twice, and then the residue was dissolved in 3 ml of tetrahydrofuran to yield a solution of 103a in tetrahydrofuran. Compound 105 (200 mg, 0.35 mmol) was dissolved in 5 ml of tetrahydrofuran, and 0.23 ml of a 1.55 M solution of n-butyl lithium in n-hexane was added slowly thereto dropwise at - 78 °C, and then the mixture was stirred at - 78 °C for one hour. Then, to the solution was added slowly the solution of 103a in tetrahydrofuran obtained above dropwise at - 78°C, and the mixture was stirred at - 78 °C for 30 minutes. The reaction mixture was allowed to warm to room temperature with stirring over 1.5 hours. The mixture was evaporated to dryness in vacuo , and the residue was distributed between 1 N hydrochloric acid and ethyl acetate, and the organic phase was washed with an aqueous saturated sodium bicarbonate solution and a saturated brine, dried (Na 2 SO 4 ), and then concentrated in vacuo to yield 0.28 g of a colorless oil. The oil was purified by preparative thin layer chromatography (KGF (Merck), Developer: ethyl acetate : n-hexane = 1:2) to yield 138 mg of the benzhydryl ester of 104a (54.7%). NMR (CDCl 3 ): 2.08, 2.13, 2.17, 2.20, 2.25, 2.38 (CH 3 , each s), 3.57, 3.81, 3.96 (OCH 3 , each s), 7.02-8.09 (aromatic H, multiplet) The dibenzhydrol ester of 104a (138 mg, 0.1 mmol) was dissolved in 5 ml of methylene chloride, and the solution was cooled to 0 °C, and a solution of anisole (46 mg, 0.43 mmol) and trifluoroacetic acid (110 mg) in 1 ml of methylene chloride was added to the solution. After the resultant solution was stirred at 0 °C for five hours, the reaction mixture was allowed to warm to room temperature, and evaporated to dryness to yield 181 mg of a colored sticky oil. The oil was purified by preparative thin layer chromatography (KGF (Merck), Developer: chloroform - methanol = 3:1) to yield 68 mg of 104a (64%). M.p. 278-280 °C (dec.) (recrystallized from 80% aqueous ethanol). Elementary Analysis (for C 60 H 62 O 18 S· 1 / 2 H 2 O) Theory (%): C;64.96, H;5.76, S;2.79 Found (%) : C;64.80, H;5.71, S;2.88 NMR (CDCl 3 ): 2.14, 2.18, 2.25, 2.29, 2.36, 2.40 (CH 3 , each s), 3.83, 3.86, 3.97 (OCH 3 , each s), 8.095 (aromatic H,d,J=2Hz), 7.60 (aromatic H,dd,J 1 =2,J 2 =8.8Hz), 7.05(aromatic H,d,J=8.8Hz) Example 21 Bis[3-[4'-[(4''-carboxy-3''-methoxy-2'',5'',6''-trimethyl)phenoxycarbony]-3'-methoxy-2',5',6'-trimethyl]-phenoxycarbony-4-methoxyphenyl] sulfone The dibenzhydryl ester of 104a (200 mg, 0.14 mmol) was dissolved in 5 ml of chloroform, and to the solution was added 120 mg (0.56 mmol) of m-chloro-benzoic acid (80%) under cooling with ice-water, and the mixture was stirred for 4.5 hours. The reaction mixture was washed with an aqueous 5% sodium thiosulfate solution, an aqueous saturated sodium bicarbonate, and water, successively, then dried, and the solvent was evaporated in vacuo to yield 260 mg of the crude sulfone dibenzhydryl ester. The dibenzhydryl ester of 104b (260 mg, 0.18 mmol), and 90 mg of anisole (0.83 mmol) were dissolved in 5 ml of methylene chloride, and the solution was cooled to 0 °C, and then a solution of trifluoroacetic acid (210 mg, 1.8 mmol) in 1 ml of methylene chloride was added thereto with stirring. After the solution has been stirred at 0°C for 3 hours, the reaction mixture was distributed between 1 N-HCl and ethyl acetate. The organic phase was washed with water, dried (Na 2 SO 4 ), and then evaporated to dryness in vacuo , to yield 248 mg of the crude product. The product was recrystallized from ethanol (99%) to provide 123 mg of 104b . Yield: 80.9%. M.p. 294-295°C (dec.) Elementary Analysis (for C 60 H 62 O 20 S· 1 / 2 H 2 O) Theory(%): C;63.04, H;5.49, S;2.81 Found (%): C;62.94, H;5.55, S;2.80 NMR(CDCl 3 ): 2.12, 2.16, 2.23, 2.27, 2.34, 2.38(CH 3 , each s), 3.80, 3.85, 4.04(OCH 3 , each s), 8.65(aromatic H, d, J=2.6Hz), 8.20(aromatic H, dd, J=2.2Hz, J=9.0Hz), 7.20(aromatic H, d, J=9.0Hz) Example 22 Bis[3-[4'-[(4''-carboxy-3''-methoxy-2'',5'',6''-trimethyl)phenoxycarbony]-3'-methoxy-2',5',6'-trimethyl]-phenoxycarbony-4-methoxyphenyl] sulfoxide The dibenzhydryl ester of 104a (200mg, 0.14 mmol) was dissolved in 5 ml of methylene chloride, and to the solution was added 30 mg (0.14 mmol) of m-chloro-benzoic acid (80%) under cooling with ice-water, and the mixture was stirred at 0 °C for 4.5 hours. The reaction mixture was washed with 5% aqueous sodium thiosulfate, an aqueous saturated sodium bicarbonate, and water, successively, and dried (Na 2 SO 4 ), and then the solvent was evaporated in vacuo to yield 245 mg of the crude product. The product was purified by column chromatography (40 g of SiO 2 , eluent: ethyl acetate - n-hexane (2:1)) to yield 151 mg of 104c -dibenzhydryl ester. The dibenzhydryl ester of 104c (146 mg, 0.1 mmol), and 50 mg of anisole were dissolved in 5 ml of methylene chloride, and then 110 mg of trifluoroacetic acid in 1 ml of methylene chloride were added thereto with stirring whilst cooling with ice-water. After the solution had been stirred at 0°C for five hours, the reaction mixture was evaporated to dryness in vacuo , and the residue was distributed between 1 N HCl and ethyl acetate. The organic phase was washed with water, dried (Na 2 SO 4 ), and the solvent was evaporated in vacuo , to yield 126 mg of the crude product. The product was recrystallized from ether - n-hexane to provide 118 mg of the crude compound 104c . Then, the compound was further recrystallized from ethanol (99%) to provide 95 mg of 104c . M.p. 193-5°C Elementary Analysis (for C 60 H 62 O 19 S·H 2 O) Theory: C;63.33, H;5.72, S;2.69 Found : C;63.37, H;5.67, S;2.82 NMR(CDCl 3 ): 2.11, 2.16, 2.23, 2.27, 2.34, 2.38(CH 3 , each s), 3.81, 3.85, 4.01(OCH 3 , each s), 8.35(aromatic H, d, J=2.2HZ), 7.92(aromatic H, dd, J=2.2, J'=9.0Hz), 7.21(aromatic H, d, J=9.0Hz) Example 23 Bis[3-[4'-[(4''-pivaroyloxymethyloxycarbonyl-3''-methoxy-2'',5'',6''-trimethyl)phenoxycarbonyl]-3'-methoxy-2',5',6'-trimethyl]- phenoxycarbonyl-4-methoxyphenyl] sulfide A mixture containing 500 mg (0.45 mmol) of 104a , 265 mg (1.09 mmol) of pivaroyloxymethyl iodide, 216 mg (1.56 mmol) of potassium carbonate and 25 ml of acetone was stirred at room temperature for 16 hours. To the mixture was added 200 ml of ice-water, and the resultant mixture was extracted with ethyl acetate. The organic phase was washed with water, dried (Na 2 SO 4 ), and the solvent was evaporated in vacuo , to yield 0.65 g of the crude product. The product was recrystallized from ethyl acetate - n-hexane to provide 0.516 g of 104d . M.p. 120°C (dec.) Elementary Analysis (for C 72 H 82 O 22 S· 1 / 2 hexane) Theory(%): C;65.69, H;6.73, S;2.28 Found (%): C;65.53, H;6.53, S;2.33 Example 24 3-Carboxy-4-methoxyphenyl{3'-[4''-(4'''-carboxy-3'''-methoxy-2''',5''',6'''-trimethylphenoxycarbony)-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl]-4'-methoxyphenyl]} sulfide [Step 1] Compound 102a (1.31 g, 3.9 mmol) was dissolved in 50 ml of methylene chloride, and to the solution was added 0.8 g (4.1 mmol) of diphenyldiazomethane whilst cooling with ice-water, and the mixture was stirred for 1.8 hours. The solvent was evaporated in vacuo , and the residue was applied to column chromatography (SiO 2 , 150g), and it was eluted with benzene - ethyl acetate (5:1) and then chloroform - methanol (9:1), to yield 1.06 g of 106 (54%). [Step 2] Compound 106 (300 mg, 0.6 mmol) and 761 mg of oxalyl chloride (0.52 mmol) were reacted by a procedure similar to that of Step 1 in Example 20, to provide 9 ml of the solution of 107 in anhydrous tetrahydrofuran. Then, 341 mg (0.6 mmol) of 105 in 15 ml of the anhydrous tetrahydrofuran, and n-butyl lithium in hexane (0.37 ml, 1.55 M) were subjected to a condensation reaction according to Step 2 in Example 20, to yield 688 mg of the crude product. The product was purified by silica gel chromatography (40 g of SiO 2 , eluent: ethyl acetate - n-hexane (1:2)), to yield 213 mg (0.2 mmol) of the dibenzhydryl ester. The dibenzhydryl ester obtained above was dissolved in 10 ml of methylene chloride, and deprotection was conducted using 231 mg (2 mmol) of trifluoroacetic acid and 100 mg (0.92 mmol) of anisole according to the procedure of Step 2 in Example 20, to yield 123 mg of 108 . M.p. 192-194 °C (recrystallized from aqueous ethanol). Elementary Analysis (for C 38 H 38 O 12 S) Theory(%): C;63.40, H;5.30, S;4.36 Found (%): C;63.50, H;5.33, S;4.46 NMR (CDCl 3 ) : 2.14, 2.19, 2.25, 2.29, 2.35, 2.39 (CH 3 , each s), 3.83, 3.85, 3.97, 4.08 (OCH 3 , each s), 8.08 (aromatic H,d,J=2.4Hz), 8.18 (aromatic H,d,J=2.4Hz), 7.60 (aromatic H,dd,J=2.4Hz,J'=8.8Hz), 7.55 (aromatic H,dd,J=2.4Hz,J'=8.8Hz), 7.03 (aromatic H,d,J=8.8Hz), 7.05 (aromatic H,d,J=8.8Hz) Example 25 Bis[3-(4'-carboxy-3'-methoxy-2',5',6'-trimethylphenoxycarbonyl)-4-methoxyphenyl] sulfide ( 110b ) Compound 102a (150 mg, 0.45 mmol) and 0.4 ml of oxalyl chloride (4.6 mmol) were reacted in a procedure similar to that of Step 1 in Example 20, to provide 3 ml of the solution of 103a in anhydrous tetrahydrofuran. Then, 338 mg (0.9 mmol) of 109 which had been dissolved in 50 ml of the anhydrous tetrahydrofuran, and 0.56 ml of n-butyl lithium in hexane (1.55 M, 0.9 mmol) were subjected to a condensation reaction according to Step 2 in Example 20, to yield 504 mg of the composition, which was purified by silica gel chromatography (35 g of SiO 2 , eluent: ethyl acetate - n-hexane (2:3)), to yield 0.464 g of the dibenzhydryl ester of 110b . The dibenzhydryl ester (0.464 g) obtained above was dissolved in 10 ml of methylene chloride, and deprotection was conducted using 215 mg of anisole (2 mmol) and 503 mg of trifluoroacetic acid (4.4 mmol) according to the procedure of Step 2 in Example 20, to yield 227 mg of 110b . M.p. 237-239 °C (recrystallized from benzene). Elementary Analysis (for C 38 H 38 O 12 S· 1 / 2 benzene) Theory(%): C;65.06, H;5.51, S;4.11 Found (%): C;64.98, H;5.45, S;4.23 NMR(CDCl 3 ): 2.08, 2.09, 2.32 (CH 3 , each s), 3.81, 3.95 (OCH 3 , each s), 8.09 (aromatic H,d,J=2.4Hz), 7.60 (aromatic H,dd,J=2.4Hz,J'=8.8Hz), 7.03 (aromatic H,d,J=8.8Hz) Example 26 Bis[3-[4'-carboxy-3'-methoxy-2',5',6'-trimethylphenoxycarbonyl)-4-methoxyphenyl] ether ( 110a ) Compound 102b (150 mg, 0.47 mmol) and 0.41 ml of oxalyl chloride (47 mmol) were reacted in a procedure similar to that of Step 1 in Example 20, to provide 5 ml of the solution of 103b in anhydrous tetrahydrofuran. Then, 355 mg (0.94 mmol) of 109 which had been dissolved in 15 ml of the anhydrous tetrahydrofuran, and 0.59 ml of n-butyl lithium in hexane (1.55 M, 0.94 mmol) were subjected to a condensation reaction according to Step 2 in Example 20, to yield 539 mg of the composition, which was purified by silica gel chromatography (35 g of SiO 2 , eluent: ethyl acetate - n-hexane (2:3)), to yield 490 mg of the dibenzhydryl ester of 110a . The dibenzhydryl ester (490 mg, 0.47 mmol) obtained above was dissolved in 5 ml of methylene chloride, and deprotection was conducted using 228 mg (2.1 mmol) of anisole and 534 mg (4.7 mmol) of trifluoroacetic acid according to the procedure of Step 2 in Example 20, to yield 214 mg of 110a . M.p. 194-5 °C (recrystallized from aqueous ethanol). Elementary Analysis (for C 38 H 38 O 13 ·H 2 O) Theory(%): C;63.28, H;5.38 Found (%): C;63.33, H;5.59 NMR (CDCl 3 ): 2.11, 2.12, 2.33 (CH 3 , each s), 3.82, 3.95 (OCH 3 , each s), 7.73 (aromatic H,d,J=3Hz), 7.27 (aromatic H,dd,J=3Hz,J'=9.2Hz), 7.06 (aromatic H,d,J=9.2Hz) Example 27 Bis[3-[4'-[(4''-carboxy-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl)-3'-methoxy-2',5',6'-trimethylphenoxycarbony]-4-methoxyphenyl] ether ( 120 ) [1] Preparation of the intermediate 118a Compound 115 (200 mg, 1.1 mmol) was dissolved in 5 ml of DMF, and 46 mg of NaH (60 % in oil) (1.15 mmol) was added thereto, and the mixture was stirred for 1.5 hours. After 690 mg (3.36 mmol) of copper bromide (I) - dimethyl sulfide complex was added to the mixture, 270 mg of the bromide 117 (1.1 mg) which had been dissolved in 0.5 ml of DMF was added thereto, and the resultant mixutre was refluxed with stirring for 22 hours. After cooling, the reaction mixture was distributed between 1 N HCl and ethyl acetate, and the organic phase was washed with N-HCl, water, and saturated brine, successively, and then dried (Na 2 SO 4 ). The solvent was evaporated to dryness, and the residue (383 mg) was applied to silica gel chromatography (40 g of SiO 2 , eluent: ethyl acetate : hexane (2:3)), to yield 163 mg of 118a . M.p. 94-95 °C (ethyl acetate - hexane (2:3)) Elementary Analysis (for C 18 H 18 O 7 ) Theory(%): C;62.26, H;5.25 Found (%): C;62.42, H;5.24 Alternatively, the compound 118a may be obtained by the following procedure. A mixture containing 200 mg (1.1 mmol) of 115 , 272 mg of 117 (1.1 mmol), 167 mg of K 2 CO 3 (1.2 mmol), and 4 ml of pyridine was heated to 130 °C (oil bath) with stirring for 15 minutes, and then 87 mg of cupric oxide (II) (1.1 mmol) were added thereto, and the mixture was heating to 140 °C (oil bath) with stirring for 23 hours. After coolong, the reaction mixture was diluted with 50 ml of ether, and the resultant precipitate was filtered off, and then the filtrate was washed with the dilute hydrochloric acid, water, and saturated brine, successively, and then dried (Na 2 SO 4 ). The solvent was evaporated to dryness, and the residue (293 mg) was subjected to silica gel chromatography, to yield 161 mg of 118a . Yield: 42.3%. [2] Preparation of the intermediate 118b Compound 118a (684 mg, 2 mmol) was dissolved in 10 ml of methanol, and 5 ml of 1 M potassium hydroxide aqueous solution was added thereto, and then the mixture was heated under reflux for 5 hours. The reaction mixture was evaporated to dryness in vacuo , and the residue was distributed between 1 N HCl and ethyl acetate. After the organic phase had been washed with water and dried (Na 2 SO 4 ), the solvent was evaporated to dryness, and then the residue (560 mg) was recrystallized from methanol, to yield 268 mg of 118b . M.p. 178-180 °C Elementary Analysis (for C 16 H 14 O 7 ) Theory(%): C;60.32, H;4.47 Found (%): C;60.38, H;4.43 [3] Preparation of the title compound Compound 118b (100 mg, 0.31 mmol) and 0.3 ml of oxalyl chloride (3.4 mmol) were reacted in a procedure similar to that of Step 1 in Example 20, to provide 5 ml of the solution of 119 in anhydrous tetrahydrofuran. Then, 357 mg (0.63 mmol) of 105 which had been dissolved in 10 ml of the anhydrous tetrahydrofuran, and 0.39 ml of n-butyl lithium in hexane (1.59 M, 0.62 mmol) were subjected to condensation reaction according to Step 2 in Example 20, to yield 490 mg of the crude product. The compound was purified by silica gel chromatography (45 g of SiO 2 , eluent: ethyl acetate - n-hexane (2:3)), to yield 443 mg of the dibenzhydryl ester of 120 . The dibenzhydryl ester of 120 (440 mg, 0.31 mmol) obtained above was dissolved in 5 ml of methylene chloride, and deprotection was conducted using 150 mg (1.4 mmol) of anisole and 370 mg (3.2 mmol) of trifluoroacetic acid according to the procedure of Step 2 in Example 20, to yield 285 mg of 120 . M.p. 258-260 °C (recrystallized from aqueous ethanol). Elementary Analysis (for C 60 H 62 O 19 · 1 / 2 EtOH) Theory(%): C;65.80, H;5.75 Found (%): C;66.00, H;5.90 NMR (CDCl 3 ): 2.16, 2.20, 2.26, 2.29, 2.36, 2.38, 2.40 (CH 3 , each s), 3.83, 3.86, 3.97 (OCH 3 , each s), 7.74(aromatic H,d,J=3.2Hz), 7.29(aromatic H,dd,J=3.2HZ,J'=9.2Hz), 7.08 (aromatic H,d,J=9.2Hz) Example 28 Trans-1,2-bis[4'-(4''-carboxy-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl)-3 ' -methoxy-2',5',6'-trimethylphenoxycarbony] ethene ( 121a ) Compound 105 (2.0 g, 3.5 mmol) was dissolved in 80 ml of anhydrous tetrahydrofuran, and 2.2 ml of n-butyl lithium in hexane (1.59 M) was added dropwise slowly at - 78 °C. The mixture was stirred at -78 °C for 50 minutes, and 270 mg of fumaryl chloride (0.19 ml, 1.77 mmol) which had been dissolved in 3 ml of tetrahydrofuran was added dropwise thereto. After the mixture was stirred at - 78 °C for additional 4.5 hours, the solvent was evaporated in vacuo , and then the residue was distributed between 1 N HCl and ethyl acetate. The organic phase was washed with water, dried, and then the solvent was evaporated in vacuo , to yield 2.267 g of the crude dibenzhydryl ester. The crude product (2.0 g) obtained above was dissolved in 50 ml of methylene chloride, and 0.82 g (7.6 mmol) of anisole and 1.87 g (16.4 mmol) of trifluoroacetic acid were added thereto at 0 °C, and the mixture was stirred at 0 °C for 3.5 hours. The solvent was evaporated in vacuo , and the residue was washed with ether to provide 1.24 g of 121a as a colorless solid. The compound was recrystallized from N,N-dimethylformamide. M.p. above 300 °C. Elementary Analysis (for C 48 H 52 O 16 · 1 / 2 H 2 O· 1 / 2 DMF) Theory(%): C;64.01, H;5.91 Found (%): C;64.01, H;6.09 NMR (d 6 -DMSO): 2.45, 2.48, 2.52, 2.53, 2.53, 2.70 (CH 3 , each s), 4.07, 4.11 (OCH 3 , each s), 7.78 (aromatic H,s) Example 29 1,2-Bis[4'-[4''-carboxy-3''-methoxy-2'',5'',6''-trimethylphenoxycarbonyl)-3'-methoxy-2',5',6'-trimethylphenoxycarbony] ethane ( 122 ) The dibenzhydryl ester 121b (100 mg) which had been obtained in Example 28 was dissolved in a mixture consisting of 5 ml of ethyl acetate and 0.5 ml of acetic acid, and the resultant mixture was reduced at atmospheric pressure using 20 mg of 10% Pd-carbon as catalyst. The catalyst was filtered off, and the precipitated crystals were dissolved in chloroform - methanol, and the solution was combined with the above filtrate. The mixture was evaporated in vacuo , and the residue was distributed between ethyl acetate and 1 N HCl, and the organic phase was washed with water, dried (Na 2 SO 4 ), and then evaporated to dryness in vacuo to yield 92 mg of a colorless solid. The solid was recrystallized from chloroform - n-hexane to yield 42 mg of 122 . M.p. > 270 °C (dec.). Elementary Analysis (for C 48 H 54 O 6 ) Theory(%): C;65.00, H;6.14 Found (%): C;65.17, H;6.11 NMR (d 6 -DMSO): 2.04, 2.07, 2.17, 2.18, 2.19, 2.32 (CH 3 , each s), 3.18 (CH 2 ,s), 3.72, 3.73 (OCH 3 ,s,6H each) Example 30 [Step 1] Thielavin B (5.0 g, 8.82 mmol) was dissolved in 100 ml of trifluoroacetic acid, and the solution was cooled to 0 °C. To the solution was added 1.5 g of hexamethylenetetramine (0.01 mmol), and the mixture was stirred at 0 °C for one hour, and then it was heated at 50 °C (bath temperature) with stirring for 4.5 hours. The reaction mixture was evaporated to dryness in vacuo , and to the residue were added 300 ml of water, and then the mixture was heated at 60 °C (bath temperature) with stirring for 6 hours. After cooling, the precipitated solid was filtered off, washed thoroughly with water, and then dissolved in about 200 ml of ethyl acetate. The solution was dried over Na 2 SO 4 , and the solvent was evaporated in vacuo to yield 5.34 g of a foam. The foam was dissolved in 100 ml of chloroform, and 1.94 g of diphenyldiazomethane was added thereto at 0 °C, and the mixture was stirred for two hours. The solvent was evaporated in vacuo , and the residue (5.84 g) was subjected to silica gel chromatography (350 g of SiO 2 , eluent: ethyl acetate - n-hexane (1:4)), to yield 2.32 g of 123 . [Step 2] Compound 123 (2.03 g, 2.67 mmol) obtained above was dissolved in 50 ml of methanol, and 0.5 g (0.013 mol) of solid NaBH 4 was added thereto in small portions at room temperature, and after the addition was completed, the resultant mixture was stirred for an additional 1.5 hours. The reaction mixture was concentrated in vacuo , and the residue was distributed between 1 N HCl and ethyl acetate. The organic phase was washed with saturated brine, dried (Na 2 SO 4 ), and then evaporated in vacuo to yield 2.22 g of the crude product. The product was recrystallized from a mixture consisting of ethyl acetate and n-hexane (1:3) to yield 1.75 g of 124 . NMR (CDCl 3 ): 2.09, 2.12, 2.17, 2.21, 2.25, 2.40, 2.65 (CH 3 , each s), 3.57, 3.83 (OCH 3 , each s), 5.03 (CH 2 OH,br.S), 8.50 (s,OH), 11.65 (s,OH) [step 3] Compound 124 (1.5 g, 2 mmol), and the benzhydryl ester of thielavin D (1.4 g, 2 mmol) were dissolved in 150 ml of toluene, and 0.24 ml (2 mmol) of boron trifluoride diethyl ether complex was added thereto, and the mixture was stirred at room temperature for 30 minutes. The reaction mixture was diluted with 150 ml of ethyl acetate, washed with water, and then dried (Na 2 SO 4 ). The mixture was evaporated in vacuo , and the residue (3.61 g) was dissolved in 40 ml of methylene chloride, and to the solution were added 0.96 g of anisole and 2.2 g of trifluoroacetic acid whilst cooling with ice-water, and the mixture was stirred for one hour. The resultant mixture was left to stand overnight, and then the solvent was evaporated in vacuo . To the residue was added dilute hydrochloric acid, and the precipitated oil was collected by decantation and dissolved in ethyl acetate. The ethyl acetate phase was washed with water, dried (Na 2 SO 4 ), and evaporated in vacuo to yield 3.79 g of the crude product. The product was purified by silica gel chromatography (eluent: chloroform - methanol - acetic acid = 500:50:25), to yield 1.234 g of the thielocin B3. Thielocin B3 : recrystallized from an aqueous ethanol, m.p. 205-207 °C (dec.) IR (KBr): 3400, 1740, 1710, 1648, 1610, 1280, 1145 cm -1 NMR (CDCl 3 ): 2.13, 2.18, 2.26, 2.29, 2.35, 2.41, 2.65, 2.89 (CH 3 , each s), 3.85, 3.86 (OCH 3 , each s), 6.37(aromatic H,s.), 11.52, 12.52 (O H , each s, disappeared by adding D 2 O) Elementary Analysis (for C 62 H 66 O 20 ·2H 2 O) Theory(%): C;64.03, H;6.22 Found (%): C;63.80, H;6.05 Example 31 To a solution of thielocin A1 (6.5 mg) in 0.3 ml of chloroform were added trimethyl ortho-formate (1.8 ml) and p-toluene sulfonic acid (60 mg), and the mixture was left to stand at room temperature for five days. The reaction mixture was distributed between ethyl acetate and water, and the organic phase was dried (Na 2 SO 4 ), and evaporated in vacuo . The residue was purified by thin layer chromatography (Developer: CHCl 3 : MeOH : water 62:25:4, Rf: 0.3) to yield 5.3 mg of 125 . M.p. 198-200 °C. HRMS MNa + bbsd m/z 1061. 3763 (Theory: 1061.3779 for C 56 H 62 O 19 ) The compounds of the present invention were tested for their Phospholipase A 2 inhibitory activity by the following procedure. 1-Palmitoyl-2-[1- 14 C]-linoleoyl L-3-Phosphatidylethanolamine (Amersham, Inc., 59 mCi/mmol) were diluted with L-α-phosphatidylethanolamine (Sigma, Co., from egg albumin) [2,000 dpm/nmol], and the dilution was sonicated. The resultant dilution was used as a substrate. The PLA 2 (phospholipase A 2 ) which was used in the test was from rat platelets. The PLA 2 and the substrate preparation were added to a solution of CaCl 2 (3 mM) in Tris-buffer (0.1 M, pH 7.4), and the mixture was allowed to react at 37 °C for 20 minutes. Then, the reaction was terminated by adding 1.25 ml of Dole's reagent to the reaction mixture and stirring immediately the resultant mixture. To the mixture was added 0.5 ml of distilled water and 0.8 ml of n-heptane, and the mixture was stirred, centrifuged, and the supernatant was taken into another tube. To this supernatant were added an additional 0.8 ml of n-heptane and silica gel, and the mixture was stirred, centrifuged, and the supernatant was taken into vials. Toluene cocktail was added to the vials. The amount of free fatty acid released by PLA 2 was determined using a liquid scintillation counter. Inhibitory activity (%) was estimated by the formula: [(DPM value at the addition of the inhibitor - DPM value without PLA 2 ) / (DPM value with only PLA 2 - DPM value without PLA 2 )] × 100. The results are shown in the following Table 1. PLA 2 inhibitory activity [IC50(µM)] Compound No. Rat platelets 8 > 330 9 > 480 52 0.16 50 4.6 44 0.13 48 3.0 54 0.88 59 1.45 56 0.035 62 0.95 74 0.070 77 0.34 104a 0.049 108 2.1 104b 0.050 121 0.33 120 0.042 110b 2.5 104c 0.12 110a 3.4 122 0.04

Claims: Thielocin derivatives of the formula: wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 are independently hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen; E 1 and E 2 are independently hydrogen, or an ester residue; m and n are independently an integer of 0 to 4; -Y- is a bivalent group which is selected from the group consisting of the following radicals: -CH 2 CH 2 - , -CH=CH- , provided that m + n is not zero, when Y is -CH 2 CH 2 -, -CH=CH-, wherein X is a single bond, CH 2 , O, S, SO, or SO 2 ; R 9 and R 10 are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, or R 9 and R 10 may be combined together to form methylene, ether, sulfide, sulfinyl, or sulfone; R 9' and R 10' are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, or R 9' and R 10' may be combined together to form methylene, ether, sulfide, sulfinyl, or sulfone; R 11 is hydrogen or lower alkyl; R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , R 26 , R 27 , R 28 , R 29 , R 30 , and R 31 are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, provided that one of R 9 , R 15 , R 16 , R 17 and R 18 , one of R 10 , R 12 , R 13 and R 14 , one of R 9' , R 23 , R 24 , R 25 and R 26 , one of R 10' , R 19 , R 20 , R 21 and R 22 , and one of R 27 , R 28 , R 29 , R 30 and R 31 are a single bond; R 32 , R 33 , R 34 , R 35 , and R 36 are independently hydrogen, lower alkyl, lower alkoxy, hydroxy, halogen, or Z, and R 37 is hydrogen or an amino-protecting group, wherein Z is a single bond or a bivalent group of the formula: R 38 , R 39 , R 40 , R 41 , and R 42 are independently a single bond, hydrogen, lower alkyl, lower alkoxy, hydroxy, or halogen, provided that one of R 32 , R 33 , R 34 , R 35 and R 36 is always Z, and when Z is not a single bond, one of R 38 , R 39 , R 40 , R 41 and R 42 is a single bond, or when two or more of R 32 , R 33 , R 34 , R 35 and R 36 are Z, only one of Zs and R 38 , R 39 , R 40 , R 41 and R 42 is a single bond; or the salts thereof. A pharmaceutical preparation for oral or external use which preparation comprises a thielocin derivative as claimed in claim 1 in association with a pharmaceutical carrier. A pharmaceutical preparation as claimed in claim 2 comprising daily dosage of 0.1 mg to 500 mg of a thielocin derivative according to claim 1. A pharmaceutical preparation as claimed in claim 3 suitable for oral administration and comprising a daily dosage of from 0.5 mg to 100 mg of a thielocin derivative according to claim 1. A pharmaceutical preparation as claimed in claim 3 or claim 4 in unit dosage form comprising from one to five portions making up said daily dosage. Thielocin derivatives as claimed in claim 1, for use as phospholipase A 2 inhibitors.

Inventors: FUJII YASUHIKO, MATSUTANI SHIGERU, OHTANI MITSUAKI, SHIRAHASE KAZUHIRO, TANAKA KAZUSHIGE, YOSHIDA TADASHI, FUJII, YASUHIKO, MATSUTANI, SHIGERU, OHTANI, MITSUAKI, SHIRAHASE, KAZUHIRO, TANAKA, KAZUSHIGE, YOSHIDA, TADASHI, TANAKA, KAZUSHIGE SHIONOGI SEIYAKU TERAE-RYOU
Applicants: SHIONOGI & CO, SHIONOGI & CO., LTD.
Application year: 1992
Main classification: C07C65
IPC codes: C07D309, C07C317, C07C229, C07C69, C07D493, C07C323, C07C271