ARYL MACROCYCLIC COMPOSITIONS FOR TREATING VIRAL INFECTIONS

Abstract: A composition for use in inhibiting cell infection by an enveloped virus, by administering to an infection site, a therapeutically effective amount of a macrocyclic compound composed of aryl ring subunits connected one to another by ring-attached bridge linkages, and containing sulfonic acid-derived substituents carried on non-bridge atoms of the subunits. In one preferred embodiment, the composition also includes an anti-viral nucleoside analog compound.

Description: ARYL MACROCYCLIC COMPOSITIONS FOR TREATING VIRAL INFECTIONS 1. Field of the Invention The present invention relates to a method for inhibi¬ ting cell infection by enveloped viruses. 2. References Allen, R.M. , Clin Neuropharmacol, 6:S64 (1983). Barre-Si oussi, F. , et al., Science 220:868-871 (1983). Blackburn, G.M., et al., Chemica Scripta, 26:21 (1986) . Chanock, R.M. , et al., Am.J.Hyg. 66:29-300 (1957). Collins, P., J Antimicrob Chemoth, 5:431 (1979). Dick, E.C., Proc. Soc. Exp.Biol.Med. 127:1079-1081 (1968) . Erlich, K.S., et al., N.Eng.J.Med.320:293-296 (1989). Elion, G.B., et al., Proc.Natl.Acad.Sci USA 74:5617- 5620 (1977) . Galbraith, A. . , Lancet, 1026 (1969). Gibrack, CD., et al. , J.Inf.Dis. 146:673-682 (1982). Gottlieb, M.S., et al. , N.Eng.J.Med. 305:425-3 (1981). Hansch, C. in Drug Design (E.J. Ariens, ed.). Vol. II, p. 271, Academic Press, (1971). Hansch, C, Leo, A., Structure-Activity Correlation, Wiley, (1979) . Hilleman, M.R., Proc.Soc.Exp.Biol.Med. 85:183-18 (1954) . Huttunen, p., et al, Pharmacol Biochem & Beha 24:1733-38 (1986). Jaffe, Chem. Rev. , 53, 191 (1953). Kern, E.R., A er.J.Med. 73:100-108 (1982). Klatzmann, D., et al.. Science 225:59-63 (1984). Lifson, J.D., et al.. Science 241:712-716 (1988). March, J., Advanced Organic Chemistry 3 ri ed. , Chapter 9, Wiley (1985) . Martin, J.C., ed. Nucleoside Analogues as Antiviral Agents, ACS, Washington D.C. (1989) . Mertz, G.J., et al., JAMA 260:201-206 (1988). Mitsuya, M., et al. , Proc.Natl.Acad.Sci.: 82:7096- 7100, USA (1985) . Po, B-L, et al.. Tetrahedron Letters, 30(8):1005 (1989) . Po, B-L, et al.. Tetrahedron, 46(10) :3651 (1990). Po, B-L, et al.. Tetrahedron, 46(12) :4379 (1990). Popovic, M. , et al. Science 224:497-500 (1984). Roizman, B., et al. Inter. Virol. 1(6:201-217 (1981). Roizman, B., et al, J. Virol. 15:75-79 (1961). Rowe, W.P., et al. , Proc.Soc.Exp.Biol.Med. 84:570-573 (953). Sidwell, R.W., App Microbiol, 22:797 (1971). Smith, K.O., et al., Antimicrob Agts Chemother 22:55 (1982) . Smith, R.A., et al., "Ribavirin: A broad spectrum antiviral agent : In : Stapleton, T., Editor, Studies With a Broad Spectrum Antiviral Agent. International Congress and Symposium Service (London) , Royal Society of Medicine, 3-23 (1986) . Spear, P.G. [Roizman, B., Editor], The Herpes Simple Viruses, Vol. 3, Plenum Press, New York, pp. 315-35 (1989). Stannard, L.M., et al., J. Gen. Virol., 68:715-72 (1987). 3. Background of the Invention The challenge in developing an effective therapy an prophylaxis for viral disease is to achieve inhibition o viral processes without producing extreme side effects an preferably without inducing viral resistance. Since viral replication requires use of the cellular apparatus of the host, treating virus infection by inhibiting viral replica¬ tion can be lethal to the infected host cells as well. Ideally, the virus should be destroyed or inactivated in the host prior to its invasion of host cells. This is normally accomplished, with varying degrees of success, by the host's immune system, but this mechanism requires an earlier immune response, either by a prior infection or by vaccination. Further, many viruses, such as Herpes Simplex viruses (HSV) are able to effectively elude a host's immune systems, and at least one virus, the human immunodeficiency virus (HIV) is known to cripple the host's immune system (Gottlieb) . Currently, the most widely used anti-viral agents are nucleoside analogs. This class of drugs acts by disrupting viral replication, either by inhibiting enzymes required for nucleic acid processing, or by producing defective viral genomes, such as by premature termination of replica- tion. As an example, acyclovir, a purine analog used in treating a variety of viral diseases, including herpes simplex virus-1 (HSV-1) and herpes simplex virus-2 (HSV-2) inhibits viral replication at several key points, including inhibition of viral thymidine kinase and DNA polymerase, and DNA strand elongation (Elion) . Ribavirin, another purine analog, is the drug of choice in treating respirat ry syncytial viruses (RSV) infection. This compou appears to act by reducing cellular GTP levels, blocki the action of several GTP-dependent viral process (Smith) . To date, the most common drug treatment of H infection is with zidovudine (Azidothy idine; AZT) , thy idine analog which is particularly effective agains human retroviruses. AZT acts with high affinity to bloc viral RNA-dependent DNA polymerase (reverse transcriptase) but does also block human DNA- polymerase and causes chai termination (Mitsuya) . Other nucleic acid analogs include ganciclovir vidarabine, idoxuridine, trifluridine and foscarnet (a inorganic phosphate analog) . As indicated above, all o these drugs, by blocking viral replication, also have th capacity to disrupt and normal host replication and/or DN transcription processes as well (see, e.g., Martin). Understanding of the mechanisms of infection an replication of viruses has lead to alternate drug thera pies, including attempts to block viral entry into cells, alter protein synthesis at the host ribosomes, complexatio of viral DNA/RNA, and immunomodulation. Interferons ar glycoproteins which have complex actions including enhance ment of certain immune responses as well as direct antivi ral action. They are more competent in preventing infec tion, rather than treating established viral infection, an their use leads to undesirable problems including acute, serious discomfort, bone marrow suppression, viral resis tance,and development of host immune response to th interferon. Treatment with "anti-sense M polymers of nucleic acid is a method in which the particular viral genome is th select target. The treatment provides a highly discrimi nating approach which would be expected to have minimal side-effects; its use as a therapeutic is hampered by problems of targeting, introduction into cells, and th quantity of material that would be required to block eac strand produced. Agents which bind to and interfere wit host ribosomal protein synthesis will block viral replica tion. These include the toxin ricin, various plan proteins such as pokeweed anti-viral protein, alpha sarcin and other low molecular weight compounds. The weaknes with the use of these materials is their lack of selectivi ty. In the treatment of HIV, additional therapy has bee developed by specifically targeting the unique retrovira enzyme, reverse transcriptase. Non-retroviral systems d not produce or use this enzyme, but the virus canno replicate without it. In some instances, understanding of structural aspect of the mechanisms of replication of viruses has provide additional drug therapies. Certain viruses, includin orthomyxoviruses and paramyxovirus, herpes viruses, togaviruses and retroviruses, contain a viral envelop which surrounds the viral capsid and nucleic acid. Durin cell infection by an enveloped virus, the plasma membran of the host cell is altered to include some viral-code proteins and, as the viral nucleoprotein core exits th host cell in which it was assembled, it becomes envelope with the modified membrane, thus forming the viral envelop. Because this structure is unique to host cells when the are virally infectious and distinct from normal cells, i can serve as an additional target for therapeutic assault. 4. Summary of the Invention The present invention includes a composition for use in inhibiting cell infection by enveloped viruses. The composition includes, in a suitable carrier, a macrocyclic compound composed of aryl ring subunits connected one to another by ring-attached bridge linkages which form a continuous chain of connected backbone atoms. The subunits have sulfonic acid-derived substituents on non-backbon atoms of the aryl subunit rings. The composition i formulated for oral, topical, or parenteral administratio of the compound. The ring subunits in the macrocyclic compound prefera bly include naphthalene subunits with sulfonic-acid derive substituents at the 3 and 6 ring positions, phenyl subunit with sulfonic acid-derived substituents at the 4 rin position, where the bridge linkages in the macrocycle ar between the 2 ring-carbon position of one naphthalene o phenyl group, and the 7 ring-carbon group of an adjacen naphthalene group or 5 ring-carbon position of an adjacen phenyl group. The compound preferably includes 4-8 suc subunits. The sulfonic acid-derived substituent i preferably sulfonic acid, a sulfonate salt, sulfinic acid, a sulfinate salt, a sulfone, or a sulfonamide. In one general embodiment, the macrocyclic compoun includes at least 4 naphthalene subunits, each havin sulfonic acid-derived substituents at 3 and 6 ring-carbon positions, polar groups at 1 and 8 ring positions, and bridge linkages between the 2 ring-carbon position of one subunit and the 7 ring-carbon position of an adjacent subunit. One preferred compound of this type has the general structure: where R j is sulfonic acid, sulfonate salt, sulfinic acid, a sulfinate salt, an alkyl sulfone, or a sulfonamide, such as SO j NHR, where NHR is NH j , NHOH, or an amino acid, R 1 is OH, =0, an alkyl or aryl ether, ester, or acid, or a mixture thereof, and n - 4, 6, or 8, and R 4 is >CHR M or ≥CR", where R" is H or carboxylic acid group. In another general embodiment, the macrocyclic compound includes at least 4 phenyl subunits with para- position sulfonic acid derived substitutents, bridge linkages between the 2 ring-carbon position of one subunit and the 6 ring-carbon position of an adjacent subunit. One preferred compound of this type has the general structure: where R j , R j and R 4 are as above. For use in treating infection by an enveloped virus, the compound may be administered topically, e.g., in the treatment of herpes simplex infection. For treatment of systemic enveloped virus infection, the compound may be administered orally or parenterally. Such treatment method may further include administering to the subject a dose of protamine sulfate sufficient to reduce anti-coagulant effects of the compound. In another embodiment, the composition containing the macrocyclic compound also includes an antiviral nucleoside analog compound. The macrocyclic and nucleoside analog compounds may be formulated in an ointment vehicle, for topical administration, e.g., in treating lesions due to HSV-I or HSV-II. Alternatively, the compounds may formulated in liquid or tablet form for oral administra tion, for treatment of systemic viral infection, or i solution form for systemic administration. Also A__ forming part of the invention are novel compounds compose of aryl ring subunits which are connected by ring-attache bridge linkages which form a continuous chain of connecte atoms making up the backbone of the macrocycle, and whic contain, on non-backbone atoms of the aryl subunits sulfonic-acid derived substituents selected from the grou consisting of an alkyl sulfone, and a sulfonamide of th form SO j NHR, where NHR is NH j , NHOH or an amino acid. TIBE and other objects and features of the invention will becom more fully apparent when the following detailed descriptio of the invention is read in conjunction with the accompany ing drawings. Brief Description of the Drawings Figure 1 shows the general structure of a macrocycli compound composed of naphthalene subunits, for use in th present invention; Figures 2A and 2B show non-oxidized (2A) and partiall oxidized (2B) forms of the Figure-1 structure, where n= and the subunit is chromotropic acid; Figures 3A and 3B illustrate two general methods o synthesis of a macrocyclic compound like the one shown i Figure 2A; Figures 4A and 4B show an unoxidized (4A) and partial¬ ly oxidized (4B) macrocycle with mixed phenyl and sulfonat- ed naphthalene subunits; Figure 5 illustrates reaction methods for converting the sulfonic acid substituents of macrocyclic chromotropic acid to glycyl sulfonamide and sulfonamide groups; Figure 6 illustrates a reaction method for convertin the sulfonic acid residues of macrocyclic chromotropic aci to sulfinic acid or its methyl (aryl) ester; Figure 7 shows the general structure of a macrocycli compound composed of phenyl groups with para-positio sulfonic acid-derived substitutents, for use in the presen invention; Figure 8 shows a non-oxidized form of the Figure-7 structure, where n=4 and the subunit is parasulfonic acid; Figures 9A and 9B illustrate general methods o synthesis of non-oxidized and partially oxidized forms of the Figure 8 compound; Figure 10 shows a reaction scheme for replacing th ring hydroxyl groups in the Figure-8 compound with acetyl groups; Figure 11 shows a reaction for converting sulfonic acid substituents to a glycyl sulfonamide group in a phenyl-subunit macrocyclic compound; Figure 12 shows a reaction scheme for producing a macrocyclic compound like that shown in Figure 8 but with carboxylic acid-containing bridge linkages; Figure 13 shows a reaction scheme for replacing hydroxyl groups in the Figure-8 compound with carboxylic acid groups. Figures 14A and 14B are plots of HSV viral yields, as a function of drug dose, for the macrocyclic compounds KY-1 (14A) and KY-42 (14B) ; Figure 15 shows the inhibition of -^-labeled HSV-1 binding to cells by the compound KY-1; Figure 16 is a plot of the inhibition in plaque formation of HSV-1 virus when the virus is exposed to the compound KY-1 before (open squares) , (ii) after (closed squares) , and during (closed circles) incubation with Vero cells; Figure 17A shows SDS-PAGE autoradiograms of HSV proteins in the presence (lane A) and absence (lane B) mercaptoethanol, and of HSV-2 proteins in the presen (lane C) and absence (lane D) of mercaptoethanol, all wit bound radiolabeled KY-1, and stained marker proteins (lan E); Figure 17B shows SDS-PAGE autoradiograms of radiola beled KY-1 compound bound to HSV-1 glycoproteins gD (lane A and B) , gB (lanes C and D) , and gC (lanes E and F) ; Figures 18 (A-D) show plots of effects of topical Y- on epithelial damage (18A) , conjunctivitis (18B) , iriti (18C) , and stromal disease (18C) subsequent to ocula application of HSV-1 in rabbits; and Figures 19A and 19B show the drop in HSV-1 (19A) an HSV-2 (19B) viral yields when infected cells are exposed t increasing concentrations of Y-l alone (solid circles) increasing concentrations of acyclovir alone (open cir cles) , increasing concentrations of acyclovir plus 25 μg/m Y-l (solid rectangles) , and increasing concentrations o acyclovir plus 50 μg/ml Y-l (solid ovals) . Detailed Description of the Invention I. Definitions The terms defined in this section have the followin meanings unless otherwise indicated. An "enveloped virus" means a virus containing proteinaceous viral envelop which surrounds the viral capsid. Such enveloped viruses include orthomyxoviruses and paramyxovirus, herpes viruses, togaviruses and retro- viruses. During cell infection by an enveloped virus, the plasma membrane of the host cell is altered to include some viral-coded proteins and, as the viral nucleoprotein core exits the host cell in which it was assembled, it becomes enveloped with the modified membrane, thus forming the viral envelop. A "aryl ring" subunit is single ring or fused rin structure containing at least one aromatic ring, i.e., a 5- or 6-membered ring with the 6 pi electrons necessary for aromaticity. Examples include benzene, naphthalene, mixed aromatic and non-aromatic fused ring structures, such as tetralin, and heterocyclic structures, including fused-ring structures, such as quinoline, isoquinoline, and indole. A "macrocyclic compound composed of aryl ring sub- units" is a cyclic compound formed by linking ring atoms in aryl ring subunits to form a cyclic chain. A "ring-attached bridge linkage" is a linkage between a ring atom of one aryl subunit to a ring atom of an adjacent aryl subunit in a macrocyclic compound; The ring-attached bridge linkages and the (shorter path of) ring atom joining bridge linkages in the subunits collectively form a "continuous chain of connected backbone atoms". In the compound illustrated in Figure 1, the chain is formed by the bridge linkages (R 4 ) to positions 2 and 7 of the naphthalene rings and the 5 ring atoms in naphtha- lene between positions 2 and 7. In the compound illustrat¬ ed in Figure 7, the chain is formed by the bridge linkages (R 4 ) to positions 2 and 6 of the benzene rings and the 3 ring atoms in benzene between positions 2 and 6. Similarly, the "non-chain ring atoms" in the macro- cycle are the ring atoms which are outside the bridge linkages. In the compound illustrated in Figure 1, the non-chain atoms include the 5 naphthalene ring atoms from ring positions 3-6; in the Figure-7 compound, the 3 ring atoms from positions 3-5. A "sulfonic acid-derived substituent" includes sulfonic acid, a sulfonic acid salt, sulfinic acid, sulfinate salts, alkyl and aryl sulfones, sulfonamides of the form SO j NHR, where R is H or a substituent having an OH, ether, ester, ketone, or acid moiety, and SH. II. Preparing Arvl-Subunit Macrocyclic Compounds This section describes the synthesis of two genera types of aryl macrocyclic compounds which are useful in th anti-viral treatment method of the invention. The firs type is composed of naphthalene subunits with sulfoni acid-derived substituents, described in subsection A. Th second general type is composed of phenyl subunits havin para-position sulfonic acid-derived substituents, describe in subsection B. From the synthetic routes given in th two sections, it will be apparent how macrocycles compose of mixed subunits, e.g., both naphthalene and pheny subunits can be prepared. The synthetic methods are als generally applicable to macrocycles composed of heterocy clic subunits with sulfonic acid-derived substituents. A. Macrocyclic Compounds with Substituted Naphthalene Subunits Figure 1 shows the general structural formula of a macrocyclic compound composed of substituted naphthalene subunits, for use in the present invention. One exemplary compound of this type is shown in non-oxidized (I) and partially oxidized (II) form in Figures 2A and 2B, respec¬ tively. The compound is a tetra er of chromotropic acid (1,8-dihydroxy, 3,6-disulfonic acid naphthalene) subunits linked by methyl or methylene (>α^ or ≥CH) bridges ( ^ . As seen, the methylene bridges and the "interior" ring atoms (ring positions 1, 2, 7, and 8) form a continuous chain having attached R x = OH or =0 groups attached at the 1 and 8 positions. The non-chain atoms (ring positions 3-6 on each substituent) have R 2 = sulfonic acid substituents on the 3 and 6 ring atoms. The nature of the partially oxidized structure was deduced from H 1 and C 13 NMR studies, and from mass spectroscopy evidence. For purposes of the following discussion, and for illustrating synthetic routes, usually only the non- oxidized subunit form of the compound is given. It will b understood that the compound may be partially oxidized after exposure to air under heat and acidic conditions i.e., contain one or more R x ketone (-0) groups, and double bond between the ring and the associated bridg methylene group, as indicated in Figure 2B. It will als be understood that the same reaction mechanisms will appl generally to the partially oxidized form of the compound i.e., the structure shown in Figure 2B, or similar struc tures containing additional R 1 =0 groups, except that R modification reactions will typically selectively modify a R j -OH group, and leave the corresponding R j =0 grou intact. As will be seen below, the compound preferabl includes the chromotropic acid derivatives in which j i a polar substituent, such as OH, =0, CO^I or an ether, thioether, ester, or thioester linked alkyl or aryl group, and combinations of these group, e.g., where only the O groups in the partially oxidized structure are substitute by one of the above groups. R j , as noted, is a sulfonic acid-derived substituen which may be sulfonic acid, as shown in Figure 2, sulfonate salt, sulfinic acid (-SO j H) , and sulfinate salts, sulfones, and sulfonamides. S ] is H or an uncharged or negatively charged substitu ent, but subject to the activity constraints discusse below. Also as will be seen below, the * bridge linking the chromotropic acid derivative subunits is preferably of th form >CHR or ≥CR (indicating unsaturated bridges in the partially oxidized form) , where R is H or a small carbon- containing group, such as lower alkyl, alkenyl, ketone, or carboxylic acid group, or aryl group. The bridge may also be of the form where R' is similarly H or a small carbon containing group, such as a lower alkyl group. Alternatively, the bridges in the macrocycle may b ring structures, including aryl ring structures, such as i the dimeric macrocycle shown in Figure 4, or analogou structures formed by bridging through heterocyclic rings such as pyrole or furan rings. The number of subunits may vary from 4 (e.g., Figure 4 structure) to 8, with macrocycles containing 4, 6, and subunits being preferred. In the reaction schemes de scribed below, the macrocycle formed may include mixture of compounds with different subunit numbers (n) values, e.g., a dominant n=4 structure (4 subunits) with additional structures containing 6 and 8 subunits. Representative macrocyclic compounds which have been synthesized and tested for anti-viral activity are iden- tified by their R t , R^ 3 , and * substituents in Table 1 below. The KY and Y number in the lefthand column in the table re ers to the analog designation of the corresponding compound. For example, the compound in which R x is OH, j is S0 2 H 2 , R 3 is H, and , is -CH j - is designated KY-3. Although not shown in the table, the compounds may exist in a partially oxidized state in which one of more R t groups are -0, and adjacent bridges contain a double-bond carbon linkage to the ring. Table 1 KY R3 KY-175 KY-176 KY-193 KY-194 KY-270 KY-272 KY-276 KY-277 KY-280 KY-281 KY-284 KY-285 KY-288 KY-289 KY-290 KY-291 KY-293 KY-294 KY-307 KY-346 KY-352 KY-357 KY-359 KY-395 KY-397 KY-398 KY-399 Y-20 Y-34 Y-66 KYY-19 Figiαres 3A and 3B illustrate two preferred synthetic methods for preparing macrocyclic chromotropic acid com¬ pounds. The method illustrated in Figure 3A involves cyclization of a chromotropic acid derivative (including chromotropic acid itself) with an aldehyde (RCHO) to for a macrocyclic compound, such as the tetramer shown Figur 2, in which the chromotropic acid subunits are linked by R substituted methylene groups, i.e., in which is >CH (including ≥CR) . This synthetic scheme provides a conve nient method for constructing macrocyclic compounds havin a variety of different bridge-methylene R groups, b carrying out the cyclization reaction in the presence of e aldehyde of the form RCHO. For example, to construct a macrocyclic compound with a -CH j - bridge, such as the KY-1 compound (IV) , chromotropi acid (III) is reacted with formaldehyde. Typical reactio conditions are given in Example 1A for the synthesis of KY 1. Similarly, KY-42 is prepared by cyclization wit glyoxylic acid (Example IC) ; KY-48, in the presence o glyceraldehyde; KY-85, in the presence of benzaldehyde; KY 97, in the presence of acrolein; and KY-110, in th presence of pyruvic aldehyde. It will be appreciated that a variety of other RCHO aldehydes having small alkyl, alkenyl, acid and other hydrocarbon R groups would be suitable. Further, the R bridge group may be further modified after the cyclization reaction. For example, KY- 193 may be prepared by bromination of the KY-97 compound. In the method illustrated in Figure 3B, cyclization of the chromotropic acid derivatives (III) is carried out by reaction with hexamethylenetetramine, to form a 3-atom chain bridge of the type -CH 2 N(CH 3 )CH 2 - (V). The cycliza¬ tion reaction for the synthesis of KY-346 is given in ~~ Example 1J. The -CRfl (CH^) CΑ - bridge may be modified, after the cyclization reaction, to form a variety of N- substituted bridges of the -CB^NfR'JCI^-, where R' is one of a variety of small carbon-containing groups, according to known synthetic methods. Some of the bridges in the partially oxidized structure will have the form =CHN(R')CH j - As noted above, the Figure-4A compound (VI) is represen tative of macrocyclic naphthalene having a cyclic bridge, in this case a phenyl bridge. The compound is formed b reacting chromotropic acid, in the presence of hydrochlori acid with 1,2-benzenedimethanol in acetic acid, as detaile in Example 3. Similar methods can be employed to linked chromotropic acid subunits by other cyclic bridges, such as furan, pyrole, and the like. Figures 4A and 4B show the non-oxidized (VI) and partially oxidized (VIII) forms of the compound) . For synthesis of macrocyclic compounds with selected lf j , and R j substituents, two general approaches are avail¬ able. In one approach, the chromotropic acid derivative is modified after cyclization so that the cyclized product will either contain the selected R t , R j , and R 3 substituent, or contain a substituent which can be readily modified to the selected substituent. This approach is illustrated by the synthesis of KY-3, which has an SO y NH- j substituent, as detailed in Example IB. Here cyclized chromotropic acid (VIII) is reacted first with chlorosulfonic acid, to form the corresponding R 2 = S0 2 C1 derivative (IX, Figure 5) . The macrocyclic compound is then reacted with ammonia water to form the desired j = S0 2 NH 2 derivative (X, Figure 5) . A similar strategy was employed for the synthesis of KY- 357 (R j - SO^CI^CO j H) by final subunit reaction with glycine (XI, Figure 5) , at basic pH. Figure 6 illustrates the conversion of sulfonyl groups of cyclized chromotropic acid to sulfinyl (XII) and alkyl sulfone or methyl sulfinyl ester (XIV) . The first stage of the reaction involves the formation of the corresponding sulfonyl chloride derivative (IX), as outlined above. This compound is then treated with sodium sulfite, to form the corresponding sulfinyl salt (XII) . Reaction with dimethyl sulfate in the presence of sodium bicarbonate produces the corresponding methyl sulfone (XIV) . Similarly, macrocyclic compounds with a variety of R substituents may be prepared by modification of chromotro pic acid after cyclization. In synthesizing KY-151, fo example, (R t = OCH j ) cyclized chromotropic acid is reacte with dimethylsulfate under basic conditions, as detailed i Example IF, to form the dimethylether of cyclized chromo tropic acid. Similarly, in preparing KY-307 (R j OfCH^ j CO j H) , cyclized chromotropic acid is first converte to the diether of hexanoic acid by initial reaction o cyclized chromotropic acid with 6-bromohexanoic acid unde basic reaction conditions. As further examples, in preparing compounds such as KY- 272 and KY-294, in which R t has the form OCOR, the macrocy¬ clic compound formed by cyclization of chromotropic acid is reacted with an acid chloride of the form RCOC1, under basic conditions, as detailed in Example 1J for the synthesis of KY-285. In a second general approach, the selected substituent is formed on the subunit naphthalene rings by derivatization of the naphthalene subunit, with subsequent subunit cyclization to form the desired macrocycle. For the synthesis of KY-175 ( j = SO 3 CH 3 ) , chromotropic acid is reacted with sulfonylchloride, as above, to produce the corresponding R 2 = S0 2 C1 substituents. Further reaction with NaOCH j leads to the desired j substituent. Reaction details are given in Example 1H. It will be appreciated that the synthetic method for forming selected-substituent macrocyclic compounds may include both prior derivatization of chromotropic acid and subsequent derivatization of the subunits after cyclizati¬ on. For example, in forming KY-397 (R t = OCH j , R 2 = SO j NH j ) , chromotropic acid subunits are first reacted at the R 1 positions, to form the dimethyl ether derivative as described above. After cyclization with formaldehyde, the compound is further derivatized at the j position, also as described above, to convert the S0 3 group to the desire SO j NH j substituent. The KY compounds described above can be converted readil to a variety of sulfonic acid or sulfonate salts, b reaction in acid or in the presence of a suitable salt, according to well known methods. Thus, for example, several of the KY compounds shown in Table 1 are ammonium salts formed by cation exchange of protons in the presence of an ammonium salt, such as ammonium chloride. In addition, exposure of the macrocyclic compound to a variety of metal cations, such as the cations of Ca, Ba, Pt, Cu, Bi, Ge, Zn, La, Nd, Ni, Hf, or Pb, may produce both a metal salt and a metal chelate of the macrocyclic compound in which the metal is chelated at interior polar pocket in the compound. The physical properties of several macrocyclic compounds prepared in accordance with the invention have been studied by absorption and mass spectrometry and by nuclear reso¬ nance spectroscopy (NMR) , as detailed in Examples 1A, IB, IC, and IJ. These compounds include tetrameric macrocyclic compounds, such as indicated in Figure 2, or mixtures with predominantly tetrameric forms. B. Macrocyclic Compounds with Substituted Phenol Subunits Figure 7 shows the general structural formula of a macrocyclic compound composed of substituted phenol subunits, for use in the present invention. One exemplary compound of this type is shown in Figure 8, which is a tetramer of phenol parasulfonic acid subunits linked by methylene bridges (XV) . As seen, the methylene bridges and the "interior" ring atoms (ring positions 2, 1, and 6) form a continuous chain having R } = OH groups attached at the 1 ring positions. The non-chain atoms (ring positions 3-5 on each substituent) have R j = sulfonic acid substituents on the 4 ring atoms. Figure 9A illustrates a general method for formin macrocyclic compounds of this type. The macrocycli precursor shown at the left (XVI) is a class of compounds known generally as tert-butyl calix(n)arenes, where n is the number of phenolic subunits (with para-position t-butyl substituents) in the macrocycle, and the bridge connections are methylene groups, t-butyl calixarenes with 4, 6, and 8, subunits are commercially available. In the sulfonation reaction shown in Figure 9A, a t-butyl calixarene with a selected subunit number is treated with concentrated sulfuric acid, typically for about 4 to 5 hours at 75-85 β C to effect substantially complete displace¬ ment of the 4-position t-butyl group by a sulfonic acid group. Details of the sulfonation reaction are given in Example 2A. The method has been used to produce the n=4 macrocycle compound shown in Figure 8, and related macrocy¬ cles with 6 and 8 phenol subunits. A similar method is used for preparing a sulfonated calixarene with partially oxidized 1-position OH groups, as shown at 9B. Here the t-butyl calixarene starting material is treated with concentrated sulfuric acid at a temperature above 100°C, preferably between 150-170 β C. The reaction is effective to sulfonate the subunit rings and to partially oxidize the interior OH groups. As indicated in Figure 9B, partial oxidation can lead to a conjugated macrocyclic structure (XVIII) in which bridge contributes delocalized electrons. This conjugated structure is colored, and the development of a colored product can be used to monitor the course of the oxidation reaction. Details of the reaction are given in Example 2B. It will be appreciated that the desired macrocycle can also be formed directly by reacting parasulfonic acid phenol (or precursors thereof) under suitable bridging conditions, such as described above for producing naphtha- lene-subunit macrocycles. This is illustrated by the reaction shown in Figure 12, for production of a macrocycl having carboxylic acid-containing bridge groups. In thi method, phenol parasulfonic acid is reacted with glyoxyli acid, under conditions similar to those described i Example IC, to form the cyclized structure shown (XXII) The macrocyclic compounds formed as above can be modi fied, according to general procedures outlined in Sectio IIA above, to achieve selected R } groups, modified sulfony groups, and/or addition of R- groups. The range of R x an j substitutents is substantially the same as that discusse above. Figures 10, 11, and 13 illustrate various reactio methods for modifying the j group of an already forme macrocycle. In Figure 10, the sulfonated structure show in Figure 8 is treated with acetic anhydride, to form an o acetyl R x group. Details of the reaction are given i Example 2C. Since this structure would be expected t undergo hydrolysis in the presence of serum esterases differences in the activity of the ester compound and th free OH compound would be expected to occur after intrave nous (IV) administration. Example 2G describes a simila reaction scheme for forming a toluene sulfonic acid este at the R j position. Figure 11 illustrates a general method for formin sulfona ides, such as glycylsulfonamide (XXI) of the Figur 8 compound. Analogous to the reactions described wit respect to Figure 5, the sulfonated phenyl macrocycli compound (XVII) is treated with chlorosulfonic acid, t form the corresponding sulfonyl chloride analog (XX) Further reaction with a selected amine, in this cas glycine, gives the desired sulfonamide. Reaction detail are given in Example 2D for the synthesis of the 3 - S0 2 NI compound and in Example 2E, for the synthesis of the glycy sulfonamide compound. Figure 13 depicts a general non-exclusive syntheti method for a net substitution of R x - OH by R, - carbo moieties. In Example 2H, the reactions detail a proces from which a substrate (R t = OH, j = tert-butyl, R 4 = CH^, n » 4) affords an intermediate (R x = CN, g = tert-butyl, = 0* 2 , n = 4) . Further modification then provides th product (R j = CXλ j H, R j = S0 3 H, R 4 = CI^, n = 4) . It will be appreciated that substituent modifications a the R 1 site can be selectively carried out at OH sites i the partially oxidized macrocycle, such as the structur shown at Figure 9B. That is, reactions which are specifi for ring OH groups will leave the =0 group intact, thus providing a mixed R χ group containing =0 groups. The R j is generally H, but may be an uncharged or negatively charged substituent, similar to the R 3 group described in Section IIA above. The R 4 bridge linking the chromotropic acid derivative subunits is preferably of the form >CHR or > CR, where R is H or a small carbon-containing group, such as lower alkyl, alkenyl, ketone, or carboxylic acid group, or aryl group, as noted above, or of the form -CH j NR'CH^-, where R' is similarly H or a small carbon containing group, such as a lower alkyl group. Alternatively, the bridges in the macrocycle may be ring structures, including aryl ring structures, analogous to the dimeric macrocycle shown in Figure 4. Also as above, the number of subunits may vary from 4 (e.g., Figure-4 structure) to 8, with macrocycles contain¬ ing 4, 6 and 8 subunits being preferred. In the reaction schemes described below, the macrocycle formed may include mixtures of compounds with different subunit numbers (n) values, e.g., a dominant n=4 structure (4 subunits) plus additional structures containing 5-8 subunits. Representative macrocyclic compounds which have been synthesized and tested for anti-viral activity are iden¬ tified by their R ,, and R 4 substituents in Table 2 below. The KY and Y number in the lefthand column in the table refers to the analog designation of the correspondin compound, as in Table 1. Compounds which are partiall oxidized at the R t position, and have which may have bot saturated and unsaturated bridge methylene carbon groups are indicated as in Table 1. The compounds shown in Table 2, and R-group combinations thereof, described above can be converted readily to a variety of sulfonic acid or sulfonate salts, by reaction in acid or in the presence of a suitable salt, according to well known methods, as described above. III. Inhibition of Virus Infectivitv This section examines the ability of compositions containing a macrocyclic compound of the invention to inhibit cell infection by a variety of enveloped viruses. The enveloped viruses which were examined are the herpes viruses. Herpes simplex virus-1 (HSV-1) and herpes simplex virus-2 (HSV-2) , which are double-stranded DNA viruses (Roizman) ; human immunodeficiency virus (HIV) , an RNA retrovirus (Popovic; Barre-Simoussi) ; and influenza A and B and respiratory syncytial viruses (RSV) , all RNA viruses (Chanock) . For comparative purposes, selectednon-envelopedviruses, including adenovirus, a double-stranded DNA virus (Rowe; Hilleman) , and rhinovirus, a single-strand RNA virus (Dick) , were examined. Typically, inhibition of virus infectivity was measured by the extent of inhibition of cytopathic effects detectable in infected cultured cells ' . Inhibition of HSV-1 and HSV-2 infectivity in cultured cells was also shown by inhibition of virus binding to infectable cells, and inhibition of viral plaque formation in infected cells, as described below. In addition, a large number of representative aryl macrocyclic compounds (including those shown in Tables 1 and 2) were examined for toxicity in cell culture, using a panel of human cell lines, as detailed in Example 4. Briefly, the selected KY- or Y- compound was added to cell cultures at a final concentration of 5, 10, 25, 50, or 100 / vg/ml. Three days later the cells were washed to remove drug, and stained with a vital stain, to determine the percentage of dead cells in each culture. The IC^ drug concentration, i.e., value concentration of drug which produced 50% cell death, was 50,vg/ml for KY-143, KY-151, and KY-163, and 100/vg/ml or greater for all of the other KY com¬ pounds tested. For KY-1, which has a molecular weight of 1404 daltons, a drug concentration of 100 μg/ml is equiva- lent to about 66 μK. A. inhibition of HSV Infectivitv: Naohthalene-Subunit Compounds Several compositions containing one of the compounds in from Tables 1 and 2 were tested for inhibition of cytopath¬ ic effects in cultured, HSV-infected cells. In the method reported in Example 5, Vero cells were infected with HSV-1 or HSV-2 and allowed to grow in culture until cytopathic effects were clearly visible. In the absence of infection. the cells form an even monolayer of fibroblast-like cells With HSV infection, a cytopathic effect characterized b round cells in suspension is clearly evident after 2 hours, followed by clumping and lysis of infected cell after 24-72 hours. In the drug inhibition study reported in Example 5, cell were exposed to HSV-1 or HSV-2 virus and, at the same time to a selected aryl macrocyclic compound, at a final dru concentration of 10 ,vg/ml. Twenty-four hours later th cells were examined for cytopathic effect. If a clea cytopathic effect was not observed with lOμg/ml of the drug the study was repeated at a drug concentration of 20 / /g/m for some compounds. Table 3 below lists 50 naphthalene-subunit macrocycle which were tested in this assay. A "+" symbol in th second column indicates that the compound was effective i inhibiting cytopathic effects at 10 or 20 μg/ml. A "- symbol indicates that CPE was observed at 10 or 20 μg/ml 1 N, no inhibition of CPE observed at highe concentration tested, or insufficient inhibition observ to predict IC^. The compounds used in Table 3 were further tested f activity against HSV infection in a plaque reduction assa as detailed in Example 6. Here Vero cells, after overnig incubation, were exposed to serial dilutions of KY co pound, from 0.625-10 μg/ml, and HSV-1 or HSV-2 virus f two hours. After washing to remove drug and extracellul virus, the cells were further incubated for 2 days, th stained and counted for plaque formation. Percent inhib tion was determined by dividing plaques produced by tot number of plaques in infected, untreated controls. Fr the concentration effect curve of plaque inhibiti (expressed as percent of control) , the concentration compound required to produce 50% plaque reduction, IC^, w determined. The IC^ values for infection by HSV-1 and HS 2 infection is given in the right-hand columns in Table With reference to the compound structures given in Tab 1, the following R-group features can be identified contributing to low activity (no protection of cells fr CPE effect seen at 10-20 μg/ml: in KY-48, KY-49 and K 110, a bulky side chain in the methylene bridge; in KY-143 an OH R j group; in KY-147 and KY-148, a sulfonamide with non-polar alkyl group at the R j position; in KY-158 and KY 175, a sulfone or sulfonyl with a non-polar alkyl group a the j position; and in KY-395, a trimethyla ine bridge i combination with a methyl ether substituent at the R position. The "GC" symbol for KY-193 means that some gian cells were formed, indicating partial inhibitory activity. Despite the lower activity of alkyl sulfone compounds, these compounds have the in vivo for conversion to th corresponding sulfonic acid compound by esterase action. Looking now at the compounds which give complete CPE a 10-20 μg/ml, the following R-group structures can be identified as preferred radicals: The j position contains OH, including combinations of OH and =0 groups; alkyl and aryl esters, including combination of such esters and =0; and alkyl ethers, including combina¬ tions of such ethers and =0. The optimal radicals at the R j position are sulfonic acid or sulfonic acid salts, sulfinic acid and salts thereof, and sulfonamides with polar amine groups, such as NH j , NHOH, N-glycosides (KY-352) , and amino acids. The preferred radicals at the R g position are H or Br. The optimal bridge linkage groups are substituted and unsubstituted methylenes, where the R group is not a bulky alkyl group, and preferably a carboxylic acid group. As a further guide to R-group selection, compounds having an ED JO value of < lμg/ml for at least one of the two HSV tests have one of the following R-group characteristics: Compounds whose R α groups are lower-alky1 ethers or esters, or contain a terminal carboxylic acid group are typically most active, especially in combination with =0 groups at other R j sites in the compound. The R j groups are sulfonic acid or sulfonic acid salts or sulfonamides with a terminal carboxylic acid. This feature indicates that an j position acid group favors hig activity. The R 4 bridge is methylene or a methylene carrying carboxylic acid (acetyl) group. The ability of selected naphthalene-subunit compounds t inhibit HSV-1 and HSV-2 viral yields at selected dru concentrations up to 10 μg/ml was assessed in the vira inhibition assay described in Example 7. Briefly, culture Hela cells were exposed to serially diluted KY compound an virus, allowed to grow for 24 hours, then freeze/thawed times to release virus particles. Vero cells were infecte serial dilutions of the viral lysates were assayed fo plaque counts as described in Example 6. The drop in vira yield, as a function of drug concentration, is plotted i Figures 14A and 14B for compounds KY-1 and KY-42 respec tively. The dose dependent drop in viral yield was betwee about 3-5 orders of magnitude, depending on drug and virus. The degree of inhibition of viral yield was generall greater for HSV-1 than for HSV-2. Similar results wer observed with several other KY compounds. B. Inhibition of HSV Activity; Phenyl Subunit Compounds A similar study of anti-HSV activities was carried ou with several of the phenyl macrocyclic compounds listed i Table 2 above, with the results shown in Table 4. As see from column 2 of the table, all of the compounds which wer tested gave inhibition of CPE at 10-20 μg/ml. Inhibitio activity of the tested compounds against HSV-I and HSV-2 i the plaque reduction assay is shown in the two righthan columns in the table (given as IC^ in μg/ml) . The highest activities observed for the phenyl-subuni compounds are comparable to the highest activities see with the naphthyl compounds, e.g., from about 1-3 μg/ml IC^ values. The most active compounds, Y-226 (n=8) , Y-48 (n=6) , and Y-225 (n=4) all have partially oxidized R t OH/=0 groups, and each partially oxidized compound is substantially more active than its corresponding non-oxidized analog. The partially oxidized n=3 compound, KY-268, is somewhat less active than its n-4, 6, and 8 analogs. Among the non- oxidized compounds, the n-8 compound, Y-l, is somewhat more active than the corresponding n=4 and n=6 compounds. Addition of acetyl groups at the R t position produces little change in the activities of partially oxidized compounds, also consistent with the results observed with the naphthalene-subunit compounds, addition of alkyl esters at the t positions gave activities comparable to the partially oxidized analog. As a general guide to optimizing compound activity in the phenyl-subunit compounds, the same rules discussed above generally apply. Thus, for example, highest activity is expected when the R 3 group is a sulfonic acid, or a sulfone or sulfonamide with a polar of acid R group. Similarly, highest activity is expected when the t group is an ether. ester, acid group, preferably in combination with =0 R groups. C: Comparison of Anti-HSV Compounds The inhibitory effect of KY-1 against drug-resistan strains of HSV-1 and HSV-2 was compared with several anti viral agents which have been used in treating HSV infec tion. These compounds tested were the nucleoside analog acyclovir (ACV) , ganciclovir (DHPG) , phosphonoformat (PFA) , and phosphomethoxyethyladenine (PMEA) . Inhibitio of viral yield was determined, as above, by infecting Hel cells in the presence of wild type or drug-resistan strains of HSV-1 or HSV-2, and serial dilutions of selected anti-viral compound, and infecting Vero cells wit serial dilutions of the Hela cell lysate, as above. Details of the inhibition study are given in Example 8. The ID,,, concentration (which effects 90% inhibition o viral yield) is given in Table 5. The KOS (HSV-1) and 33 (HSV-2) are wild type viruses; the KOS(PMEA) ' and KOS(PFA) ' are drug-resistant HSV-1 strains having a DNA polymeras mutation. The 333(DHPG) strain is a drug-resistant HSV- strain having a thymidine kinase mutation. With th exception of DHPG as an inhibitor of drug-resistant strain of HSV-1, and PMEA as an inhibitor of drug-resistan strains of HSV-2, all of the nucleoside analogs were a least about 20 times less active against drug-resistan strains than wildtype strains of either HSV-1 or HSV-2, a measured by drug concentration required to inhibit yield. By contrast, the aryl macrocyclic compound showed substan tially the same specific activity against drug-resistan strains of HSV-1 and HSV-2 as against wildtype strains. T ble 5 Drug Tested (I0 n ) ≠ The data demonstrate that aryl macrocyclic compounds ar effective against drug-resistant HSV strains at dru concentrations comparable to those which are effective against wild type virus strains. By contrast, and with the exception of DHPG as an inhibitor of HSV-1 strains, both drug-resistant strains showed a significant resistance to ACV, DHPG, PFA, and PMEA, as evidenced by the severalfold greater ID^ drug concentrations required for virus inhibi- tion. D. Inhibition of RSV and In luenza A Virus Infectivitv Representative macrocyclic compounds from Table 1 were tested for inhibition of cytopathic effects in cultured MDCK or HEp2 cells after infection by Influenza A virus (A/Taiwan strain) or RSV virus. In the method of inhibit¬ ing virus infectivity by influenza A, MDCK cells were infected with the virus, and the cells were allowed to grow in culture until cytopathic effects were clearly visible. In the absence of infection, the cells form an even monolayer of fibroblast-like cells. With virus infection, a cytopathic effect characterized by cell clumping is observed. For each compound tested, drug concentrations of 0.1, 1, 10, 25, and 100 μg/ml were added to cultured cells at the time of virus infection, as detailed in Examples 9 and 11. Twenty-four hours later the cells were examined for percent clumping, based on the percent of clumped cells of total cell particles in a given view field. The inhibi¬ tion of clumping was plotted as a function of drug con- centration, to determine the dose effective to produce 50% reduction in the percent clumped cells, measured wit respect to control (no drug treatment) . The measured ED values are given in Table 6 below. A similar method was employed to determine the ED^ of RS inhibition of cytopathic effect (cell clumping) in HEp cells, with the results shown in Table 6. Details ar given in Example 9. In general, RSV was significantly more sensitive t compound inhibition than the Influenza A/Taiwan virus. Highest-IAV activity was seen with a sulfonamide with pola amine (SO j H j ) at the R j position, and with selected methy lene bridge groups. Relatively high anti-RSV activity was seen with all compounds except KY-47. E. Inhibition of HIV Infectivitv: Naohthalene-Subunit Compounds Representative macrocyclic compounds from Table 1 were tested for inhibition of cytopathic effects in cells infected with one of two HTLV-III strains, HTLV-III B and RF II strains, as described in Example 12. Briefly, cell chronically infected with HTLV-III B or RF-II HIV strain were incubated in the presence of serial dilutions of th selected KY compound, then further cocultured with indi cator cells. The extent of syncytia formation was score under phase microscopy. The concentration effective t produce complete inhibition of syncytia formation, ED 100 , i shown in Table 7 for the two HIV strains. The "N" mean that the compound was not tested for that virus. 10 15 20 25 30 35 40 As seen from these results there is a general cor¬ relation between anti-viral activity against the two strains; that is, compounds which are most active against the HTLV-III jj strains are also most active against the RF-11 strain. With reference to the compound structures given in Table 1, the following R-group features can be identified as contributing to sub-optimal activity (ΕD X values > 63 μg/ml for both strains): in KY-48, a bulky side chain in the methylene bridge; in KY-110, a methyl ketone group in the bridge; in KY-143, an OH g group; in KY-147 and KY-148, a sulfonamide with a non-polar alkyl group at the 2 position; in KY-158 and KY-175, a sulfone or sulfonyl with a non-polar alkyl group at the R j position; and in KY-272, a methyl ester at the R t position combined with an acetyl- group bridge. These features are substantially the same as those which gave reduced activity against HSV viral infectivity, i.e., showed no inhibitory effect on CPE 10-20 μg/ml. Similarly, those factors which promote high activi against HSV activity are in general the same as those whi give highest activity against HIV infectivity. The factors include: the groups at the R x position are O including combinations of OH and =0 groups; alkyl and ar esters, including combination of such esters and =0; a alkyl ethers, including combinations of such ethers and = The preferred radicals at the j position are sulfon acid or sulfonic acid salts, sulfinic acid and sal thereof, and sulfonamides with polar amine groups, such NH j , NHOH, N-glycosides (KY-352) , and amino acids, wi sulfonic acid. In particular, high activity was seen wi sulfonic acid, sulfonate salts, and sulfonamides having terminal carboxylic acid group. The optimal radicals at the R 3 position is H, with bo OH and Br giving reduced activity. The bridge groups are preferably substituted a unsubstituted methylenes, where the R group is not a bul alkyl group. As a further guide to R-group selection, compoun having an ED^, value of < lμg/ml for at least one of the t HSV tests have one of the following R-group characteris tics: Compounds whose R, groups are lower-alkyl ethers o esters, or contain a terminal carboxylic acid group ar typically most active, especially in combination with = groups at other R t sites in the compound. The 3 groups are sulfonic acid or sulfonic acid salt or sulfonamides with a terminal carboxylic acid. Thi feature indicates that an 3 position acid group favors hig activity. The R 4 bridge is methylene or a methylene carrying carboxylic acid group. These preferred R-groups are intended to provide guidance in the selection of R groups at the ^R* posi¬ tions, for optimizing compound efficiency. F. Inhibition of HIV Infectivitv: Phenyl-Subunit Compounds Representative macrocyclic compounds from Table 2 were tested for inhibition of cytopathic effects in cells infected with one of two HTLV-III strains, HTLV-III B and RF- II strains, as described in Example 12, and in the subsec¬ tion above. The IC j ,, values measured for the HXB and RS-11 strains of HIV are given in units of μg/ml in Table 8 below. Interestingly, the Y-l compound and KY-226 (the corresponding partially oxidized analog) have comparable activities against the HXB strain, in contrast to the significantly higher activity of KY-226 seen against HSV viruses. All of the other compounds tested have partially oxidized R j -0 groups, and all compounds give comparable activity. IV. Specificity Toward Enveloped Viruses This section examines the specificity of the vira inhibition method to enveloped viruses. The studi reported in subsection A show that the macrocyclic co pounds used in the method act, at least in part, by bindi selectively to viral envelop proteins, and that thi binding blocks virus attachment to infectable cells thereby inhibiting virus infectivity. These studies ar detailed in parent U.S. patent application Serial No 647,720, filed January 29, 1991. Subsection B examines th inhibitory effect of the macrocyclic on non-envelope viruses. A. Mechanism of Viral-Infection Inhibition In one study, the ability of a macrocyclic compound t block HSV binding to infectable cells was examined a described in Example 14. Briefly, Vero cells were expose to radiolabeled HSV-1 or HSV-2 in the absence of KY com pound or in the presence of 10 μg/ml KY-1, and binding o the virus at times up to 4 hours after exposure to th virus was measured. Figure 15 shows a plot of viru (radiolabel) binding to cells over the four-hour incubatio period. In the absence of drug, the amount of bound viru increased steadily over two hours, and slightly from 2- hours. By contrast, virus binding to cells peaked at abou 1/2 hour in the presence of drug, presumably reflecting th time during which the binding events effective to bloc virus binding to the cells are equilibrating. In a second study, the effect of compound whe administered prior to, during, or after cell infection b HSV-1 was examined as described in Example 15. In thes studies, cells were exposed to one of a series of increas ing KY-2 concentrations, and the extent of infection wa measured by number of plaques observed 24 hours afte infection. The reduction in plaque formation, expressed as a percent of control, is shown in Figure 16 for cells treated with drug prior to (solid rectangles) , during (closed circles) , and after (open rectangles) . Virus inhibition was seen most significantly when the cells were treated with drug during exposure to virus, indicating that virus inhibition occurs at the period of virus binding to and entry into infectable cells. In a third study, purified HSV-1 virus suspensions were incubated with KY-1 or the sodium salt thereof, or a control solution for 1 hour, then serially diluted to drug concentrations between 10 1 to 10"* μg/ml as described in Example 16. Addition of the serially diluted virus suspensions gave the plaque counts, measured in duplicate, shown in Table 9. The "X" symbol in the table indicates plaques too numerous to count. The results of the study demonstrate that inhibition of HSV infection by KY com¬ pounds is due, at least in part, to binding of drug to HSV particles. Further, complete virus inhibition was seen at drug final drug concentration of 10" 2 to 10"* μg/ml (which are much lower than those needed to inhibit HSV in Vero cell culture) . It can be concluded that the drug-bind¬ ing/inactivation of the virus is effectively irreversible, i.e., not reversed by high dilution effects. In a fourth study, the binding of radiolabeled KY- compound to HSV-1 and HSV-2 viral proteins was examined After compound binding, virus proteins were fractionated b sodium dodecyl sulfate polyacrylamide gel electrophoresi (SDS-PAGE) , and the gel patterns developed by autoradio graphy. In Figure 17A, lanes A and B in the figure ar autoradiographs of HSV-1 proteins in the presence (lane B and absence (lane B) of mercaptoethanol, and lanes C and D analogous patterns for HSV-2 proteins. The lane at th right contains the molecular weight markers, as indicated The major bands of drug binding in HSV-1 have molecula weights, as determined from SDS-PAGE, of 45, 66, and abou 130 kilodaltons. The major bands of drug binding in HSV- have similar molecular weights. The major bands which sho KY binding in Figure 17B correspond in molecular weight, t HSV glycoproteins gD, gB, and gC. B. Effect on Non-Enveloped Viruses The ability of KY compounds to inhibit cell infectio by a rhinovirus and adenoviruses 5 and 7 which are non enveloped viruses, was similarly studied. Vero cells (10 s ) were infected with a rhinovirus in the presence of KY-1, a concentrations ranging between 1-100 μg. Twenty-four hour after virus infection, the cells were examined for cyto pathic effect, evidencing viral infection. No reduction i cell clumping was observed at any of the KY drug concentra tions tested. Vero cells were infected with adenovirus in the pre sence of KY-1, also at concentrations ranging between 1-10 μg, and twenty-four hours after virus infection, the cell were examined for cytopathic effect. No reduction in cel clumping was observed at any of the KY-1 drug concentra tions. In summary, a broad range of macrocyclic compound are effective inhibitors of cell infection by each of th several enveloped viruses which were studied. Bindin studies carried out in particular with respect to HS viruses indicate that the anti-viral activity of the com pounds is dependent on binding to virus envelope compo nents, which in turn inhibits virus attachment to infec table cells. The apparent inability of the compounds t inhibit infection of non-enveloped viruses is consistent with this mechanism. V. Viral Inhibition bv a Composition Containing a Macro- cyclic Compound and a Nucleoside Analog Compound The invention also includes a composition containing a macrocyclic compound of the type described above in combination with a nucleoside analog anti-viral compound. The nucleoside analog compound is one effective to inhibit viral replication at the level of viral replication or transcription. Among the nucleoside analog compounds which are useful in combination with a macrocyclic compound, in accordance with the invention are: (1) Pyrophosphate analogs, such as phosphoformic acid (PFA) , phosphonoacetic acid (PAA) , methanediphosphonic acid (MDP) , carbonyldiphosphonic acid (COMDP) , phosphonoglyoxa- lie acid (COPAA) , and various halogen- and/or methyl- substituted derivatives thereof, which are inhibitors of viral nucleic acid poly erases. In particular, these compounds are known to inhibit herpes virus (Blackburn, Sidwell) and Influenza (Sidwell) infections, and reverse transcriptase activity in retroviruses, such as human HIV. (2) Base-modified analogs, such as IUDR, trifluorothy- midine, AraA, and azidothymidine (AZT) , dideoxyinosine (DDI) , D4T, dideoxycytidine (DDC) , and ribavirin. Tri- fluorothymidine, IUDR, and AraA are active mainly against herpes viruses (Nicolson, 1984a, 1984b) . Ribavirin is active against several RNA and DNA viruses (Sidwell) , an AZT is active against HIV (Fischl) , as are other dideoxy nucleoside analogs, such as DDL (3) Sugar-modified analogs, such as N-acyl derivative of 5'-ioduridine, sulphonamide derivatives of 5'-amino-5' deoxythymidine, 2'-deoxy-5-ethyluridine, and N-acyl deriva tives, 5'-sulfate and 5'-sulfamate nucleoside analogs, suc as nucleocidin, adenosine 5' sulfamate, and ribavarin which may act primarily at the level of protein synthesi inhibition (Martin) . (4) Phosphate analogs, including acyclonucleosid phosphonates, such as acyclovir and gangiclovir, and thei isosteric phosphonate analogs. These compounds can act a virus-selective substrates for viral thymidine kinases, i the synthesis of nucleoside triphosphate analogs intracel lularly (Galbraith) . Subsequently, the nucleoside triphos phate analogs can act as selective substrates for viral DN polymerase, acting as a chain terminator since the analo does not have the bifunctionality necessary for chai extension (Allen) . These compounds have demonstrated anti viral activity against herpes viruses (Collins) , includin HSV-1, HSV-2, varicella zoster (VZV) , and cytomegaloviru (CMV) (Smith) . Also included in this class are phosphonomethyl ether of nucleosides, and their acyclic analogs, such as N-(3 hydroxy-2-phosphonylmethoxypropylcytosine) (HPMPC) and N (2-phosphonylmethoxyethyladenosine) (PMEA) derivatives o heterocyclic bases. These compounds act specificall against herpes viruses, adenoviruses, cytomegaloviru (DeClercq) , poxviruses, vaccinia viruses, and retroviruses. The ability of the two-compound composition to inhibi viral infection in enveloped virus is demonstrated in th study reported in Example 18, which examines the vira yields after infection of Vero cells with serial dilutions of HSV-1 or HSV-2 particles, as described above. Figure 19A shows the drop in HSV-1 viral yields when infected cells are exposed to increasing concentrations of the macrocyclic compound KY-1 alone (solid circles) , increasing concentrations of acyclovir alone (open cir¬ cles) , increasing concentrations of acyclovir plus 25 μg/ml KY-1 (solid rectangles) , and increasing concentrations of acyclovir plus 50 μg/ml KY-1 (solid ovals) . With either drug alone, a maximum decrease in viral yield was slightly less than three logs (orders of magnitude) . The effect of combined compounds was tested at two KY- 1 concentrations. At the lower KY-1 concentration of 25 μg/ml, the two compounds together gave over seven logs inhibition in viral yield, i.e., more than tenfold greater than the sum of the inhibition produced by the two drugs alone. At the higher KY-1 concentration, the combined inhibitory effect of the two compounds was several orders of magnitude greater than the sum of effect of macrocyclic compound and acyclovir alone. Similar results were observed for inhibition of HSV-2 viral yields with combined compound treatment, as seen in Figure 19B. The two compounds are formulated in tablet, ointment, or injectable form at a preferred weight ratio of between about 10:1 to 1:1 macrocyclic compound and nucleoside analog, respectively. The viral-yield plots in Figures 19A and 19B a significantly higher level of inhibition was observed when the co-administered compounds were at a ratio "~ ό ~~ about 5:1 macrocyclic compound to nucleoside. The macrocyclic compound in the composition is preferably selected for optimal activity against the target virus, e.g., a herpes virus, respiratory syncytial virus, or retrovirus, as detailed above. Similarly, the preferred nucleoside analog compound is selected for activity against the target virus (Martin) . One advantage of the combined-drug composition is that substantially lower doses of both types of compounds are required for achieving a selected viral inhibition level, reducing drug side effects in a composition that also is characterized by greater anti-viral activity. Another advantage of the combined compound composition is in over¬ coming the appearance of drug resistance to either compound alone. VI. Use of the Composition in Treatment of Viral Infection The composition of the invention includes a macrocyc¬ lic compound, as described above in Section II, contained in a pharmaceutical carrier which is suitable for oral, topical or parenteral administration of the compound. The composition may contain the macrocyclic compound alone, or in combination with an anti-viral nucleoside analog. The dosage form of the composition is one which is pharmaceutically effective, i.e., effective to inhibit viral infection of host cells. As seen above, compound doses in the range 1-50 μg/ml are generally effective in inhibiting viral infection of cells. Thus, for many applications, an effective dose is preferably one which produces a concentration of compound in this range at the site of infection. For topical administration, a composi- tion containing between 1-5% or more aryl macrocyclic com¬ pounds is suitable. In a composition containing both macrocyclic and nucleoside analog compounds, the composition dose may be substantially lower in one or both compounds, as discussed in the section above. One of the considerations in the administering the composition, particularly when the drug is administered parenterally or orally, is systemic side effects. Studies conducted in support of the present invention indicate that the macrocyclic compound, at least in the polysulfated form, can show anti-coagulant activity after oral and intravenous administration. One conclusion from these studies is that there is no bleeding complication at a concentration less than 50 μg/ml in the plasma. Another conclusion is that the anticoagulant effect of macrocyclic compounds in the bloodstream can be effectively blocked by administering a polycationic compound, such as protamine sulfate, by intravenous administration. The protamine administration is timed to correspond to highest blood levels of the aryl macrocyclic compounds. In a typical method, a dose of protamine equivalent to about 1 mg per 100 heparin anticoagulant units is administered intrave¬ nously simultaneously with . IV administration of the macrocyclic compound, or 1-2 hours after oral administra¬ tion of the macrocyclic drug. It is generally recommended that protamine be infused slowly (i.e., not more than a total of 50 mg/10 minutes) . Therefore in the case of simultaneous administration of macrocyclic compound, the rate of co-infusion of the two compounds would be adjusted such that the protamine sulfate was not introduced to the subject at a rate exceeding 50 mg/10 minutes. The composition of the invention can -therefore include protamine in an amount effective to reduce the anti-coagulant effect of the macrocyclic compound, when the compound is administered for uptake into the bloodstream. Where the composition also contains a nucleoside analog drug, and lower amounts of macrocyclic drug, the protamine may be reduced or eliminated, due to the lower amounts of macrocyclic compound. A. Iniectable Composition Studies on the pharmacokinetics and efficacy of intravenously administered composition has been studied. Briefly, it was shown that a macrocyclic compound of the type used in the method, when administered intravenously, (a) is cleared relatively slowly from the bloodstream (t 1/2 = approx. 5-8 hours) , (b) is present predominantly in free form, and (c) retains activity in the bloodstream for inhibiting viral (e.g., HSV) infection. The injectable composition contains the macrocyclic compound in a suitable IV solution, such as sterile physiological salt solution. The solution may additionally contain nucleoside analog compound and/or protamine. B. Topical Composition: Treatment of Genital Heroes fresjons For inhibiting viral infection of skin and mucosal membrane, the composition is preferably formulated in an ointment form. The use of a topical composition for treatment of genital herpes lesions is illustrated in the following study, which is detailed in Example 13. Briefly, female guinea pigs were infected intravaginally with HSV-2, then treated topically three times daily beginning 6 hours or 48 hours after inoculation with HSV-2, as described in Example 13. Althnimal groups included control animals (no treatment following virus inoculation) , placebo (vehicle treatment) , KY-1 in vehicle, or acyclovir. Swabs of vagi¬ nal secretion were obtained and assayed for viral activity by a standard CPE assay. The severity of genital lesions was scored on a 0-5+ scale through the period of primary infection (21 days) . Three to four days after HSV-2 inoculation, vesicular lesions appeared on the external genital skin. Lesions progressed to an ulcerative stage by days 7-8 and gradually healed by days 15-21. The effect of topical treatment with the KY-1 preparations on lesion development and severity is shown in Table 10. The group treated with placebo at +6h had a significantly increased lesion score-day AUC (P <0.05) ; however, mean peak lesion scores were not different when compared to the untreated control group. Lesion development as determined by both AUC values and mean peak lesion scores was significantly reduced by treatment with 5% KY-1 when given at 6h after infection compared to the placebo (P <0.001). Treatment with 1% KY-1 significantly reduced the AUC at +6h (P <0.01) but not mean peak lesion scores. No sign of any skin irritation from any of the formu¬ lations was observed. Throughout the treatment period, the genital skin remained normal in appearance; no redness or swelling was observed. The guinea pigs also remained normal and healthy in appearance throughout the entire study. In another study using the guinea pig genital model described above, animals were infected with HSV-2, then treated with KY-1 or Y-l topically at concentrations of 2% or 5% drug. Treatment of animals was initiated 6 or 24 hours post infection, as described in Example 13. Animals were treated and scored daily for severity of infection for 19 days. The effects of topical treatment with KY-1 and Y- 1 on infection are tabulated in Table 11 and compared to treatment with 5% acyclovir (ACV) . Treatment with placebo (vehicle only) resulted i significantly worse infection scores than no treatment i this study. Drug treatment with 2% or 6% Y-l, administere 6 hours post infection, resulted in reduced numbers o animals exhibiting lesions, decreased mean lesion score and decreased peak lesion score, in comparison to placeb treatment. Likewise, treatment with a 6% formulation o either KY-1 or Y-l or a 2% formulation of KY-1, adminis tered 24 hours post-infection, resulted in reduced number of lesion bearing animals and reduced severity of lesions. C. Topical Composition: Treatment of Eve Infections In another embodiment, the topical composition includes a macrocyclic compound in a ointment or solution form suitable for administering the compound to the eye, e.g., to the corneal surfaces of the eye. The composition may also include a nucleoside compound effective against the target viral infection. In one treatment method, described in Example 18, graded topical doses of compound Y-l were administered to the corneal regions of rabbits previously infected with HSV-1. Clinical slit lamp biomicroscopy was used to assess disease severity as measured by average epithelial disease involvement (Figure 18A) , conjunctivitis rating (Figure 18B) , iritis rating (Figure 18C) , and stromal disease (Figure 18D) . By day 7 post infection, the severity of the epitheli- al disease had peaked, as observed in placebo treated animals (Figure 18A) . Conjunctivitis, iritis and stromal disease parameters also progressed throughout the study (Figures 18B-D) . In all four assessments of ocular infection, drug treatment resulted in less severe infection as compared to placebo treatment. All concentrations of Y-l were effec¬ tive in reducing the development of HSV-1 induced ocular disease. Therapy with all concentrations of Y-l were statistically different from each other. A topical concentration of 12.5 μg/50 μl was the most effective ocular therapy. The epithelial disease scores decreased through day 6 post infection, and rebounded slightly on day 7 post infection. Compared to the other two Y-l therapies, this concentration was effective in reducing the development of HSV-1 disease in the eye and was associated with only mild conjunctival, iris, and stromal disease development parameters. A higher concen¬ tration (18.75 μg/50 μl) was also effective in reducing the development of corneal epithelial HSV-1-induced disease. However, this concentration of Y-l appeared to be somewhat toxic to the corneal epithelial surface and to the conjunc¬ tiva, iris and stroma. This toxicity was evidenced as an increase in all disease parameters on days 6 and 7 post infection. Viral titers were recovered from tear film (19A) a days 0, 3, 5, and 7 post-inoculation and from epithelial scrapings (19B) performed on day 7 post-infection (sacri¬ fice) . Viral titers were determined by plaque reduction and multiple regression analysis, as described in Example 10. In the tear film study, a marked reduction of viral titer was observed in all animals given topical doses of Y- 1, and this reduction appeared dose-dependent, although no difference was seen at the highest doses (12.5 and 18.75 μg/50 μl) . A dose-dependent reduction in viral titer was observed in the scrapings taken on day 7. Based upon these studies, dose efficacy/range was generated. The optimal concentration of compound appeared to be 12.5 μg/50 μl in this study. D. Oral Composition Studies conducted in support of the present invention have shown that a macrocyclic drug of the type used in the invention is available in the plasma for a period from about 0.5 hrs. after oral administration (e.g., by gavage), with a peak at about 2-4 hours. The period of effective drug concentration in the bloodstream is roughly between 4 and 18 hours after IV administration. The relatively short distribution volume halflife of the drug, reflecting distribution to extracorporeal body compartments when the compound is administered intravenously, is generally advantageous in the case where drug is one which shows anti-coagulant side effects, since the concentration of compound in the bloodstream can be more closely titrated. VI. Macrocyclic Sulfone or Sulfonamide Compounds In another aspect, the invention includes a novel macrocyclic compound of the type described above, having naphthalene-ring or phenyl-ring backbone structure in which the 2 substitution position on the naphthalene or phenyl ring is an alkyl sulfone or a sulfonamide of the for S02NHR, where NHR is NIL, or an amino acid. The alkyl sulfone may act as a prodrug, in that th ester sulfonyl ester linkage may be cleaved by esterase enzymes in the body, to release an active sulfonate compound. In addition, in vitro studies in anti-viral activity, reported above, show that the methyl sulfone compound itself is active against HIV viral infection. The amino aσyl sulfonamide compounds, e.g., KY-376, have been shown to have relatively high anti-viral activity in vitro. In addition, these compounds can be readily modified, by selection of a suitable amino acid, to produce desired solubility and charge properties. For example, the sulfonamide of aspartic acid will carry two charged carboxyl groups, whereas the sulfonamide of a basic amino acid, such as lysine, will carry both a positive and negative charge. The amino acid may be further derivatized at its C-terminus, to produce, for example, a neutral C- terminal ester group. The following examples illustrate methods of preparing tetrameric macrocyclic compounds, in accordance with the invention, and the use in inhibiting cell infection by enveloped viruses. The examples are intended to illustrate but not limit the scope of the invention. Materials All chemical reagents were obtained from Aldrich Chemical Co., or from other commercial sources. Example 1 Preparation of Naphthalene Macrocyclic Compounds A. KY-1 (R^OH, R 2 =S0 3 Na, R^H, R 4 = >CH 2 ) To a 41 mM aqueous solution (50 ml) of disodium chromotropic acid, 15 ml of 37% formaldehyde was added, giving a final molar ratio of 5:1 formaldehyde:chromotropic acid. The mixture was reacted with stirring in a stoppered flask at room temperature for 1 week. The resulting dark red solution (70 ml) was filtered under vacuum, and the filtrate, after being concentrated was precipitated by add¬ ing 200 ml of acetonitrile. The precipitated product was collected by filtration and taken to dryness under vacuum. The yield of KY-1 was 95%. The compound was characterized as follows: Melting point (M.P.)> 300 β C; HPLC in C^CN/MeOH/H j O/TFA: 14'48" single broad peak; (IR/KBr) = 3425 (OH) , 1638 (Ar) , 1181, 1044 (S0 3 ) cm" 1: UV (H j .0) : 238.0, 358.5 run Mol Weight: 1505 (M+l) by mass spectroscopy; H 1 NMR(CD 3 0D) , chemical shifts on the γ scale: 5.20 (C^, 8.01 (ArH) ppm; C u NMR (D 2 0) , chemical shifts on the r scale: 27.19, 120.18, 121.69, 122-06, 122-67, 133-30, 142.97, 154.42 and 181 ppm. Analysis: (C 22 H 10 O 1(5 S 4 Na 4 ) 2 x 6 H20 or (C 22 H n 0 16 S 4 Na 4 ) 2 x 5 H j O Found: C 33.17, H 2.54, Na 11.93 Calculated: C 32.75, H 2.23, Na 11.41; C 33.16, H 2.13, Na 11.56. B. KY-3 (R 1 =OH, R 2 "=S0 2 NH 2 , R j -H, ^ -CH j -) KY-1 (2mM) was treated with 5 ml chlorosulfonic acid and the mixture was stirred at 50°C for one-half hour. The resultant mixture was added to 20 g of crushed ice to precipitate the product, which was collected by filtration and then washed with ether. The crude product was dissolved in 100 ml of 25% ammonium water solution and allowed to react for 2 hours at room temperature. The mixture was concentrated in vacuo and the remaining oil was dissolved in a small amount of water and filtered. The product was precipitated by adding acetonitrile to the filtrate and collected by filtratio and washing with acetonitrile. The compound was character ized as follows: Melting point (M.P.)> 300 β C; Mass spec: 1452 (M-7 H 2 ) ;. HPLC in CH j CN/MeOH/H j O/TFA: 11'46" single peak; (IR/KBr) = 3430 (OH), 3187, 1686 (NH,) , 1637 (Ar) , 1211, 1110, 1044 (S0 3 ) cm" 1; UV (tt j O) : 246 nm; H 1 NMR(D 2 0), chemical shifts on the γ scale: 5.15 (CH j ) , 7.5- 8.2 (ArH) ppm; Analysis: (C 44 H 40 O 26 S 10 N 12 Na 4 )-lβl^O Found: C 28.62, H 3.93, N 8.82, S 17.17, Na 5.44; Calculated: C 28.51, H 3.89, N 9.07, S 17.28, Na 4.97; C. KY-42 (R^OH, 2 =S0 3 Na, R^H, R*= >CHCOOH) Chromotropic acid, disodium (lOmM) in 50 ml water was mixed with glyoxylic acid (10.0 mM, in 5 ml water) and 10 ml of 37% hydrogen chloride at room temperature. The mixture was boiled for 8 hours and the color of the solution turned to dark red. The resultant solution was added to 50 ml of water and filtered. The filtrate was concentrated and ethanol was added to precipitate the product of KY-42. The yield was 87%. The compound was characterized as follows: Melting point (M.P.)> 300°C; Mass spec: 1623 K-2 ) . HPLC in C^CN/MeOH/tt j O/TFA: 10'36" single peak; (IR/KBr) = 3452 (OH), 1801, 1719 (Co), 1638 (Ar) , 1206, 1050 (S0 3 ) cm "1: UV (H j O) : 238.0, 351.5, 520 nm; H 1 NMR(D 2 0) , chemical shifts on the rscale: 7.10 (CHCO^I) 8.00 (ArH) ppm; C 13 NMR (D 2 0) , chemical shifts on the r scale: 116.04 , 118.90, 120.94 , 121.27 , 122.30, 124.30 , 124.68 , 126. 60 , 128.37 , 136.48 , 136.71, 140.50 , 143.93 , 144.26 , 145.75 , 152.01, 154.33 , 156.01, 156.67 ; Analysis: (C 4g H 4 O 40 S g Na $ ) 4 -4H 2 O Found: C 32.74 , H 2.50; Calculated: C 32.58 , H 2.71 ; D. KY-123 (R^OH, R^SO^a, R^H, $,- ^H j ) KY-1 (2mM) was treated with 5 ml chlorosulfonic acid and the mixture was stirred at 50 β C for one-half hour. The resultant mixture was added to 50 g of crushed ice to precipitate the product which was collected by filtration and then washed with ether. The crude sulfonyl chloride product was treated with sodium sulfite (20 mM) in 4 ml water. The reaction mixture was kept slightly alkaline by addition at intervals of small portions of 50% NaOH for 2 days. After solvent removal, ethanol was added to preci¬ pitate the product, which was acidified by addition of 50% H j S0 4 , followed by addition of ethanol to precipitate sodium sulfate. The ethanol phase was mixed with ether (1:2, v/v) to precipitate the desired product. Product yield was 39%. E. KY-147 (R^OH, R 2 =S0 2 NHCH 3 , R^H, R^ X∑H j ) N-methyl chromotropic acid chloride was formed by reacting chromotropic acid (disodium salt) with sulpho- nylchloride in the presence of DMF. The reaction was carried out with stirring at 80 β C for 4 hours. After removal of solvent and excess of thionylchloride in vacuo, ether was added to precipitate the chromotropic acid chloride which was subsequently collected by filtration and washed with ether. The crude product was added to 20 ml of methylamine and stirred for 2 hours. After removal of all solvent from the resultant substance, the residue was dissolved in a 200 ml of cold ethanol and filtered. The filtrate was added with acetonitrile to precipitate the product chromotropic acid methyl sulfonamide. Yield 56%. The chromotropic acid methyl sulfonamide (2mM) in 3 ml water was reacted with 37% formaldehyde (1ml) at room temperature for one week. Acetonitrile was added to precipitate the product which was collected by filtration and washed by acetonitrile. Yield was 85%. F. KY-151 ( ^OCH j , R j =S0 3 Na, ^H, R<= >CH 2 ) KY-1 (50mM) was dissolved in 80 ml of NaOH water solution (0.2M NaOH) and heated to 50°C, dimethylsulfate (0.2M) was added slowly for 1 hour. The mixture was continuously stirred for another 2 hours and left at room temperature for 2 days. Saturated NaCl solution (100 ml) was added to the resultant substance and filtered. The precipitate was washed with ethanol, acetonitrile and ether sequentially. The dry substance was dissolved in 100 ml of methanol and filtered. The filtrate was concentrated and ether was added to precipitate the dimethyl ether of chromotropic acid, disodium. G. KY-158 (R^OH, R 2 =S0 2 CH 3 , R^H, R^ >CH 2 ) KY-1 from Example 1A was first treated with thionyl chloride to produce chromotropic acid sulfonyl chloride. This compound was reduced by excess sodium sulfite in the presence of sodium bicarbonate to produce the corresponding sodium sulfonate salt of cyclized chromotropic acid (R j = SO j Na) . The sulfonate salt was treated with dimethyl sulfate in the presence of NaHC0 3 , and worked up as de¬ scribed in Example 1A. Product yield was about 21%. H. KY-175 (R^OH, R^SO j CH j , j H, R 4 = >CH-.) Chromotropic acid was first treated with thionyl chlo- ride to produce chromotropic acid sulfonyl chloride. This compound was then treated with sodium methoxide in methanol in the presence of sodium salt. The product was worked up as described in Example 1A to form the macrocyclic com¬ pound. Product yield was about 29%. I. KY-285 (R^OCOCI^, ^SO^a, 3 =H, R 4 = >CH 2 ) KY-1 from Example 1A (0.66 mmole) was dissolved in 3 ml water containing 0.1 g NaOH. To this was added 1 g acetyl chloride (13 mmole) and the reaction was allowed to proceed at room temperature overnight with stirring. After solvent removal, 25 ml ethanol was added to precipitate the product. The crude product was dissolved in methanol and filtered. The filtrate was allowed to precipitate, giving a 87% yield. J. KY-346 (R^OH, 2 =S0 3 Na, R^H, R^ -CI^-NfCH^CH j ) Chromotropic acid disodium salt, was dissolved in 80 ml of water at a concentration of 50 mM with stirring at 50 β C until the solution turned to clear, hexamethylene- tetramine (50 mM) was then added to above solution with continuous stirring at the same temperature for additional two hours. At this time, the color of this mixture converted to dark blue. The mixture was allowed to stir at room temperature for 2 days. The resultant dark blue solution was filtered and the filtrate was concentrated, evaporated by flask, which was subsequently treated with 200 ml methanol to precipitate the product KY-346. The yield of KY-346 was 85%. The compound was characterized as follows: M.P.>300 β C; HPLC in CH j CN/MeOH/H j O/TFA: 13 ' 07" single peak; (IR/KBr) - 3425 (OH) , 1626 (Ar) , 1197 , 1052 (S0 3 ) cm" 1: UV (^0) : 232. 0 , 377.5 nm Analysis: (C 13 H 11 O g NS 2 Na 2 ) 4 x 12 1^0 Found: C 33 .17 , H 3 .13 , N 2.75 Calculated: C 33.98, H 3.59, N 2.96. Molecular weight: 1668 by gel filtration. Example 2 Preparation of Phenyl Macrocyclic Compounds A. Y-49 (R^OH, 2 =S0 3 H, R 4 =-CH-.-, n=4) 4-tert-butylcalix(4)arene (10 g) was treated with 200 ml of concentrated H j SO., at room temperature for 0.5 hou and then at 75-85°C oil bath for another 4 hours. The reaction was completed when no water-insoluble material was detected. The resultant oil was dropped into 500 g of crushed ice and the solution was filtered by reduced pressure. After the water removed away from the filtrate, acetonitrile (500 ml) was added to the residual and allowed to stand for 4 hours to precipitate the crude product which was then collected by filtration and washed with acetoni¬ trile, ethyl acetate and ether. Yield 8 g (73%) . The pure product was furnished by recrystallization of the crude compound with ethanol-ether or methanol-acetonitrile system. The single crystal compound was also found in the recrystallization process. Similar methods were used in the synthesis of Y-77 (R^OH, R 3 =S0 3 H, R 4 = -CH j -, n=6) and Y-l (R^OH, ^SO^, R 4 =- CH 2 -, n=8) . B. KY-225 (R t = -OH, =0) , ^SO j H, R 4 = X^, > CH, n=4) 4-tert-Butylcalix(4)arene (1 g) was treated with 10 ml of 95-98% sulfuric acid at room temperature for 0.5 hours then at 160 β C for 5 minutes. After the resultant mixture was cool, the mixture was poured slowly into 100 ml of crushed ice and filtrated. The solution was evaporated and the residual was added with 300 ml acetonitrile to produce great amount of precipitate which was collected by filtra- tion and washed with acetonitrile. The crude product was dissolved in 20 ml methanol and the product was precipitat ed by addition of diethyl ether. Yield was 84%. Similar methods were used in the synthesis of Y-48 ( j -OH or =0, 3 =S0 j H, R 4 = -CH 2 -, n=6) and Y-226 (R^ -OH or =0 2 =S0 3 H, R 4 = -CH j -, n=8) . C. O-Acetylate of Y-l (R^ -OCOC^, ^SO j H, R 4 = >CH 2 , n=8 Y-l (0.75 g) was stirred in dry acetic anhydride (3 ml) overnight. The reaction was continued until th material was dissolved in the solvent. After cooling t room temperature, the suspension was filtered. The soli was washed twice with acetonitrile and dried in vacuo. Th material was washed and recrystallized. U CNMR (D20, δ) l 173.9, 151.6, 144.1, 135.6, 130.1 34.2, and 22.4. D. Y-78 (R x = -OH, ^SO J NH J , R 4 = >CH 2 , n=8) Under nitrogen, Y-l (1 g) is heated at 60-70°C wit chlorosulfonic acid (20 ml) for 1 hour. After cooling t room temperature, the oily material is poured into ic water, and the precipitate is filtered. After washing th precipitate with cold water, the material is added to 50 m of solution containing 5.7 g glycine and 2.1 g NaOH, an stirred for 2 hours at room temperature. The crude produc was dissolved in 100 ml of 25% ammonium water solution an allowed to react for 2 hours at room temperature. Th mixture is concentrated in vacuo and the remaining oil i dissolved in a small amount of water and filtered. Th product is precipitated by adding acetonitrile to th filtrate and collected by filtration and washing wit acetonitrile. E. Glycyl sulfonamide of Y-l (R j = -OH, R J ^SOJNHCHJCO^, R 4 >CH 2 , n=8) Under nitrogen, Y-l (1 g) is heated at 60-70 β C with chlorosulfonic acid (20 ml) for 1 hour. After cooling to room temperature, the oily material is poured into ice water, and the precipitate is filtered. After washing the precipitate with cold water, the material is added to 50 ml of solution containing 5.7 g glycine and 2.1 g NaOH, and stirred for 2 hours at room temperature. After removal of all solvent from the resultant substance, the residue is dissolved in a 200 ml of cold methanol and filtered. The filtrate is added with acetonitrile to precipitate the product. F. Acetyl-Bridged Y-49 (R x = -OH, R^SO^, 4 = -CHCO j H-, n=4) 4.3 g of p-hydroxybenzenesulfonic acid was treated with g gram of glyoxylic acid in 30 ml 18% concentrated HCl for 2 hours at 100°C. After the reaction product was dried under reduced pressure, 50 ml of methanol was added and insoluble impurities were removed by filtration. The product was precipitated from the filtrate by addition of ether then collected by filtration and dried in vacuo. G. Toluene Sulfonyl Ester of Y-49 (R x = -S0 3 C 6 H 4 CH 3 , R^SO^, R 4 = >CHCθ 2 H, n=4) Under nitrogen is added toluenesulfonyl chloride (1.9 g) to a suspension of dry sodium carbonate (1.06 g) , dry dimethylformamide (10 ml) and Y-49 (0.75 g) . After an overnight reflux, the resulting mixture is cooled to room temperature and filtered. The filtrate is diluted with ether to precipitate out the crude product. Recrystalliza- tion from acetonitrile/ether solvent provided the product. H. Carboxylic Acid Derivative of Y-49 ( ^ -CO j H, R 2 =S0 3 H, R 4 = >CHC0 2 H, n=4) . Under nitrogen, trifluoromethanesulfonic anhydride (1.0 ml) is added to ice cold dry dichloromethane solution (10 ml) of 2,6, di-tert-butyl-4-methylpyridine (1.25 g) and 4-tert-butylcalix[4]arene (0.65 g) . After overnight stirring at room temperature, the mixture is diluted with pentane (10 ml) and filtered. The filtrate is extracted with ice cold IN aqueous NaOH solution, ice cold IN aqueous HCl solution, then saturated aqueous NaCl solution, dried over anhydrous sodium sulfate, filtered through a pad of silica gel and concentrated in vacuo. The residue is dissolved in a mixture of dry diisapropylethylamine (10 ml), trimethylsilyl cyanide (0.5 ml) and palladium tetra- kis-triphenylphosphine (20 mg) . After an overnight reflux under nitrogen and then cooling to room temperature, ether (50 ml) was added and the resulting suspension was fil¬ tered. After concentration of the filtrate in vacuo and silica gel chromatography (hexane/ethyl acetate eluent) , the cyano intermediate is heated at 80°C with concentrated sulfuric acid (10 ml) for 3 hours, diluted with water (10 ml) and refluxed overnight. After cooling to room tempera¬ ture, the resulting mix is added to charcoal (0.5 g) and ice (50 g) . After filtration, the resulting filtrate is concentrated in vacuo to ca 15 ml in volume and the resulting solid was filtered. The solid is dissolved in a minimal amount of methanol and precipitated out by adding ether. Final purification by reverse phase C18 chromatog- raphy (methanol/water eluent) provide the product. I. Methyl Ether of Y-l (R. =0Me, R j -SO j Na, Ε^ >CH 2 , n - 8) . A mixture of Y-l (447 mg) , NaOH (6 N in water, 1.53 ml) , and dimethylsulfate (9 ml) was heated at 60°C for 20 hours. The resulting mix was added dropwise into stirring absolute ethanol (100 ml) . The resulting suspension was centrifuged (9000 rpm, 20 min) and then the supernatant was removed. Twice, the resulting solid was dissolved in water (6 ml) , and the resulting solution was treated as above with ethanol, centrifuged, and supernatant removed. The remaining solid was lyophilized to yield the product (42 mg). K CNMR (D20, δ) : 161.2, 140.9, 137.6, 129.5, 63.6, an 33.5. 5 Example 3 Preparation of Arvl-Bridoed Macrocyclic Compound Chromotropic acid, disodium (10 g) in 55 ml of wate was -treated with 22 ml of 30 ml 37% HCl. To this solution, 0 1,2-benzenedimethanol (5 g) in 55 ml of acetic acid was added and this reaction was carried at reflex for 6 hours. After filtration of the resultant mixture, acetonitrile (500 ml) was added to precipitate the crude product and collected it by filtration. The crude compound was further purified by column chromatographic purification on LH-20 resin and elution with ethanol. Example 4 Cvtotoxicitv in Proliferating Cells A panel of human cell lines was used to check the toxicity of the drugs, including: KB (nasopharyngeal carcinoma) , HeLaS 3 (cervical epithelial carcinoma) , PLC (hepatocarcinoma) , HepG 2 (human hepatocarcinoma) HepG 2 T 14 (hepatocarcinoma transfected with HBV) , WI38 (normal human lung fibroblast) , BT549 (breast cancer) , SW480 (breast cancer) , and A549 (lung cancer) . 5 x 10 4 cells were plated in each well of a 24 well multi-dish in 1 ml of RPMI-1640 containing 5% FCS and P/S. ~ '"On the second day after plating, one of the fifty test com- pounds given in Table 3 was added to the cells, at concen¬ trations between 1-100 μg/ml. Three days later, the medium was removed and the cells were stained with Commassie Blue in 40% methanol and 7% acetic acid. The results are dis¬ cussed in Section II above. Example 5 lnhifri ς>n pf Hgv Activity cvtφpathig Effect Vero cells were maintained in RPMI-1640 medium supple mented with 5% fetal calf serum, 100 Units of penicilli per ml and 100 μg of streptomycin per ml at 37°C in humidified incubator containing 7% C0 2 . The HSV strain HSV-1 (Kos-1) and HSV-2 (333) were used. 1 X 10 5 Vero cells were plated in each well of a 96 well microtitre plate in 0.2 ml RPMI-1640 medium containin 5% FCS and 0.1% methyl cellulose (15 cps) . After overnigh incubation, and cell doubling, the medium was aspirated and replaced with 100 μl of the same medium containing 2% FCS, and 50μl control or drug solution to a final drug concentra¬ tion of lOμg/ml and 50 μl virus, containing about 3 PFU/ce- 11, i.e., 6 x 10 s PFU/well, of HSV-1 or HSV-2. The cells were cultured for 24 hours at 37°C, at which time cytopathic effects are clearly visible. In the absence of viral infection, the cells form an even monolay- er of fibroblast cells. With viral infection, the cells form a suspension of round cells, followed by cell clump¬ ing, whose appearance is easily distinguishable from normal fibroblast cells. If no detectable cytopathic effect was produced, the test was repeated with 10 μg/ml. A parallel set of cells without virus inoculation were done as a control for cytotoxicity to Vero cells. Table 1 above shows the structures of the compounds which were tested, and Table 3, column 2, the compounds which protected the cells from cytopathic effect (+) . Example 6 Inhibition of HSV Activity: Plaαue Reduction Vero cells were maintained in RPMI-1640 medium sup¬ plemented with 5% fetal calf serum, as in Example 5. 4 X 10 3 Vero cells were plated in a 24-well plate, in 1 ml RPMI- 1640 medium containing 5% FCS and 0.1% methyl cellulose (15 cps) . After overnight incubation, and cell doubling, the medium was aspirated and replaced with 100 μl of the same medium containing 2% FCS, which contained 50μl control or drug solution to a final drug concentration of 0.25, 2.5, 5, 10, or 20μg/ml and 50 μl virus, containing about lxl0 3 PFU/ml, i.e., 50 PFU/well, of HSV-1 or HSV-2, as in Example 5. After 2 hrs. at 37° absorption the virus and the drugs were removed and the cells were washed with PBS and 0.5 ml of 1% methylcellulose (4K cps) in RPMI-1640 + 2% FCS + penicillin/streptomycin (P/S) was added. Two days later, the media were removed. The cells were stained with 0.8% crystal violet in 50% ethanol. The plaques formed were counted and the percentage of inhibition was calculated by dividing by the plaques formed in control. ED^ values, indicating the concentration of drug needed to produce 50% inhibition of viral plaques, were calculated assuming a linear dose response for viral plaque inhibition. The calculated IC^ values are given in Tables 3 and 4 above. Example 7 nhibition of HSV Activity: Viral Yield Inhibition 1 X 10 6 HeLa S 3 were plated in 25 T flasks in 5 ml RPMI-1640 + 5% FCS + P/S. 24 hours later, the medium was aspirated and replaced with 6 X 10 6 PFU HSV-1 or HSV-2, and serial dilutions of selected KY compounds, at 10, 5, 2.5, 1.25, and 0.625 μg/ml drug. After growth at 37 β C for 24 hours in 2 ml of RPMI-1640 containing 2% FCS and P/S, the cells were frozen at -70 β C until the time for titration. The cells were freeze/thawed 3 times to release virus from the cells, and serially diluted 10 fold. IX 10 s Vero cells were plated in each well of 24 well multi-dish in 1 ml RPMI-1640 + 5% FCS + P/S + 0.1% methylcellulose (15 cps) . On the second day, after removal of the medium, the 10 fold serially diluted virus in 100 μl was added in duplicate. After 2 hours incubation at 37°C, the virus was removed and 0.5 ml methycellulose (4K cps) in RPMI-1640 and 2% FCS + P/S was added. Two days later, the medium was removed. The cells were stained in 0.8% crystal violet in 50% ethanol. The plaques formed were counted and the titer was calculated from the fold of dilutions. The reduction in virus yield, as a function of KY com- pound concentration, is seen in Figures 14A and 14B for KY- 1 and KY-2. Example 8 Activity Against Drug-Resistant Strains of HSV-1 and HSV-2 The following strains of HSV-1 and HSV-2 virus were used: KOS, a wild type HSV-1 virus; KOS (PMEA) and KOS (PFA) , both drug-resistant HSV-1 viruses having a DNA polymerase mutation; 333, a wild type HSV-2 HSV-2 virus, and 333 (DHPG) , a drug-resistant HSV-2 virus having a thymidine kinase mutation. Inhibition of viral yield was by KY-1, acyclovir (ACV) , DHPG, PFA, and PMEA was examined in each of the five HSV strains substantially as described in Example 7. Briefly, Hela S 3 were plated in 25 T flasks in culture, and 24 hours later, the medium was aspirated and replaced with 6 X 10 6 PFU of the selected HSV strain, and serial dilutions of KY-1, ACV, DHGP, PFA, and PMEA. After growth at 37 β C for 24 hours in 2 ml of RPMI-1640 containing 2% FCS and penicillin and streptomycin (P/S) , the cells were frozen at -70°C until the time for titration. The cells were freeze/thawed 3 times to release virus from the cells, serially diluted 10 fold, and the serial dilutions were added to Vero cells in culture. After 2 hours incubation at 37°C the virus was removed and 0.5 ml methycellulose (4 cps) in RPMI-1640 and 2% FCS + P/S was added. Two day later, the medium was removed. The cells were stained i 0.8% crystal violet in 50% ethanol. The plaques forme were counted and the titer was calculated from the fold o dilutions. From the drug dose response, the concentratio of each drug required to effect a 90% inhibition of virus yield, the IC*, concentration was determined. These values are shown in Table 5 above. Example 9 Inhibition of RSV Activity Assays to assess the antiviral activity of KY- and Y- compounds in tissue culture were performed in 96-well flat- bottom tissue culture plates (Falcon 307) , using conditions similar to those used in the cytotoxicity assays described above. In these assays, compound was tested in quadrupli¬ cate by serially diluting the compound in 2% FCS-MEM using serial two-fold dilutions (0.05 ml/well) . A 0.05 ml volume of the appropriate virus containing approximately 100 median tissue culture infectious doses (TCID^) was then added to all wells except those set aside as antiviral and tissue control wells. Next, approximately 3 X 10 3 HEp2 cells (0.1 ml) were added to each well. Control wells containing antiviral and no virus (antiviral control) , containing virus but no antiviral (virus control) , or containing medium without virus or antiviral (tissue control) , were included in each test. The challenge virus was then back titrated. All assay plates were incubated at 35°C for 5 to 7 days in a 5% C0 2 incubator. When virus control wells exhibited 70% to 100% CPE including syncytia, all wells were observed. The median efficacious concentra¬ tion (IC j o) was calculated after determining the final concentration of antiviral in the last wells in each set of quadruplicate rows exhibiting <50% CPE compared to the CP in virus control wells. The ED ∞ values calculated for eac of the compounds tested are shown in Table 5. Example 1Q Activity against HSV virus: Topical activity against in vivo ocular cultures of HSV-1 New Zealand white rabbits were acclimated for a minimum of two days prior to inoculation to allow the animals to accommodate to conditions in the vivarium facility. After the accommodation period, animals received a slit lamp ocular examination to exclude any animals with preexisting anterior segment ocular defects. Animals were bilaterally inoculated topically with an 80 μl drop of Minimal Essential Medium (MEM; Gibco) containing 10 5 pfu/ml McKrae strain HSV-1; eyes were massaged for 30 seconds. Animals were replaced individually in cages. On day 4 post inoculation (PI) , animals were evaluated by slit lamp microscopy. Corneal epithelial, iris, and conjunctival disease were graded on an increasing scale of severity from 0+ to 4+. After evaluation, animals were divided into 4 groups of 5 animals with matched corneal, stromal and conjunctival involvement. Topical therapy was initiated immediately after animal grouping. Therapy groups included: Group ≠li 5 rabbits, Y-l topical ther- apy (6.25 μg/50 μl) 5x/day for 4 days; Group #2: 5 rabbits, Y-l topical ther¬ apy (12.5 μg/50 μl) 5x/day for 4 days; Group ≠3 : 5 rabbits, Y-l topical ther¬ apy (18.75 μg/50 μl) 5x/day for 4 days; Group #4: 5 rabbits, placebo therapy (sterile water) 5x/day for 5 days. The concentration of Y-l for the ascending dose tolerance study were based upon the ED90 concentrations determined in the virus yield or CPE assays. Group 1 received topical eyedrop therapy containing 6.25 μg/50 μl [one-half of the ED90 concentration]; Group 2 received eyedrop therapy containing 12.5 μg/50 μl [the ED90 concen¬ tration]; Group 3 received eyedrop therapy containing 18.75 μg/50 μl [1.5 times the ED90 concentration]. All Y-l doses were formulated to contain these concentrations in a volume of 50 μl (a standard eye drop) . Topical therapy with 0-19 μg Y-l in 50 μl was initiat¬ ed on day 4 post-inoculation (PI) and continued to day 7 PI. All animals received daily ocular slit lamp evalua¬ tions from day 3 through day 7 PI. The ocular HSV-1 induced disease severity was recorded daily. Eyes of all animals were additionally sampled for the presence of infectious HSV-1 on days 0 (pre-inoculation) , 3, 5, and 7 PI. Briefly, tear film was obtained by swabbing the lower and upper conjunctival sacs and retain¬ ing the swab in the nasal fornix for 10 seconds. The swabs were eluted individually in Hank's Buffered Saline (HBSS, Gibco Laboratories) . Fifty microliter aliquots of the virus-HBSS eluate was adsorbed onto confluent HFF cell monolayers for 5 minutes. Monolayers were hydrated with Minimal Essential Medium (MEM; Gibco Laboratories) , incubated at 37 β C and observed daily for two weeks to detect cytopathology consistent with HSV infection (HSV CPE) . Cultures not exhibiting HSV CPE were blind-passaged to confirm negativity. On day 7 PI (sacrifice) , the corneal epithelium was scraped from the eyes and HSV was recovered on HFF cell monolayers. Corneal epithelial co-cultures were evaluate daily by inverted light microscopy. Cultures not exhibit ing HSV CPE were blind passaged to confirm negativity. Clinical efficacy of the three Y-l concentrations use in single-agent therapies were compared to placebo therapy. Virus recovery during and after topical therapy with th γ-1 formulations were compared to each other and to placebo therapy, as illustrated in Figures 18(A-G) and 19(A,B) . Example 11 Inhibition of Influenza A Activity The anti-influenza A activity of KY compounds was evaluated as described in Example 9, except that MDCK cells (kidney cell line) was used for infection in vitro by influenza virus (strain A/Taiwan) . Example 12 Inhibition of HIV-Induced Cell Fusion Human CD 4 + indicator cells (VB) and chronically infected H j cells were maintained in RPMI-1640 medium supplemented with 5% fetal calf serum, 100 Units of penicillin per ml and 100 μg of streptomycin per ml at 37°C in a humidified incubator containing 7% C0 2 . The HIV strains that were used were HTLV-III B and RF-II strains obtained from the National Institutes of Health (Bethesda, MD) . For the fusion assay, serial dilutions between 1:2 and i:2 8 of a selected KY compound, 1 mg/ml in PBS were made in a 96 well round bottom plate. The diluted KY compound was transferred to a 96 well flat-bottom plate. To each well was added 25 μg chronically infected H, cells (at 2 x 10 6 , cells/ml) , or cells chronically infected with RF-II strain HIV, followed by incubation at 37°C for 45 minutes. To each well was then added 25 μl VB cells (about 5 x 10 4 cells) , and the cells and virus isolates were cocultured for 18 hours in a humid 5% C0 2 atmosphere. The extent of syncytia formation was scored under phase microscopy, and the concentration which completely inhibited syncytia formation (ED 100 ) was recorded. The results are given in Table 6. Example 13 Effect of KY Topical Administration on Genital HSV Infec¬ tion A. Virus and Viral Inoculation The MS strain of HSV-2 was utilized for the experi¬ mental animal infection. Female Hartley strain guinea pigs (Charles River Breeding Laboratories, Kingston, NY) weighing 250-300 g were inoculated intravaginally with 2.0 x 10 5 plaque-forming units of HSV-2 one hour after being swabbed for removal of vaginal secretions. B. Treatment of Guinea Pigs In the first study, groups of 10 guinea pigs were treated topically (0.1 ml intravaginally + 0.1 ml on external genital skin) three times daily (approximately every eight hours) for seven days beginning 6h or 48h after inoculation with HSV-2. Groups of three uninfected animals were treated in a similar manner to assess any skin irritation. In a second study, groups of 8-10 animals were treated three times daily with topical formulations of 2% or 6% KY- 1 or Y-l, with treatment beginning either 6 or 24 hours following viral inoculation, as indicated in Table 8B. Formulations of KY-1 or Y-l were prepared by dissolving the compound in a 1.5% methyl cellulose solution such that final concentration of compound was 2% or 5% (wt/wt) . Control animals were given either no treatment (N=10 animals) or treatment with placebo (1.5% methylcellulose solution) . C. Sample Collection and Virus Assays To determine the effect of treatment on vaginal viral replication, swabs of vaginal secretions were obtained on days 1, 3, 5, 7 and 10 after HSV inoculation, placed in a tube containing 2.0 ml of media, vortexed and frozen at - 70°C until titrated for HSV-2. When all samples were collected, they were thawed, diluted serially and HSV-2 titers determined using rabbit kidney cells in a microtiter CPE assay. D. Evaluation of Efficacy To determine the effect of therapy on the development and spread of external genital lesions, lesion severity was scored on a 0-5+ scale through the primary infection period (19-21 days) . Lesion score-day areas and virus titer-day areas under the curve, and peak lesion scores and peak virus titers between untreated and placebo-treated or placebo-treated and drug-treated animals were compared using the Mann-Whitney U range sum test. A p-value of 0.05 or less was considered significant. The results are discussed with reference to Tables 10 and 11 in Section IV above. Animals were scored daily for 19 days following inoculation for presence of lesions and severity of lesions (on a 0-5+ point scale) . Lesion scores were tabulated as area under the curve of daily lesion score vs. time (days) -and peak lesion score observed. Data are presented in Table 11. A known antiviral agent, acyclovir (ACV) was administered in a 5% formulation to 8 animals as a positive control in the study. Example 14 Inhibition of HSV-1 Binding to Vero Cells Vero cells were maintained in RPMI-1640 medium, a described in Example 5. After overnight incubation, an cell doubling, the medium was aspirated and replaced wit 100 μl of medium containing 2% FCS composed of 50μl contro or drug solution to a final drug concentration of lOμg/m and 50 μl virus, containing about 3 PFU/cell, i.e., 6 x 10 PFU/well, of H 3 -labeled HSV-1. At time intervals of 5, 30, 60, 120, and 240 minutes, cells were removed from th suspension, washed two times with PBS, and assayed fo bound virus (cpm 3 H) . The results are given in Figure 15, where the control virus binding is indicated by soli circles, and the drug-inhibited binding, by open rectan- gles. Example 15 Effect of Dru /Virus Exposure on HSV Inhibition Vero cells were maintained in RPMI-1640 medium, as above. After overnight incubation, and cell doubling, the medium was aspirated and replaced with 100 μl of medium containing 2% FCS. In one group of wells, serial dilutions of KY-1 compound, between 0.625 and 10 μg/ml drug were added in 50 μl, together with 50μl of HSV-1 virus suspension, 5 X 10 6 PFU per well. The cells were incubated for 2 hours at 37°C, then washed with PBS and assayed for number of virus plaques, as in Example 6. In a second group of cells, serial dilutions of the drug were added to the cells, prior to the addition of the HSV-1 virus, and the cells were incubated for 2 hours at 37°C in the presence of the virus. After washing the cells to remove free drug, virus suspension was added, 5 X 10 ® PFU per well. The cells were incubated for 2 hours at 37°C, then washed with PBS and assayed for number of viru plaques, as in Example 6. In a third group of cells, 100 μl virus suspension wa added to the cells, 5 X 10 6 PFU per well, and the cells wer incubated for 2 hours at 37°C, then washed with PBS t remove unbound virus. Serial dilutions of KY-1 compound, between 0.625 and 10 μg/ml drug were added to the cells i 100 μl. The cells were incubated for 2 hours at 37°C in the presence of the drug, then washed with PBS and assayed for number of virus plaques, as above. The numbers of plaques observed in each of the above treatment methods, expressed as percent of untreated control, are plotted in Figure 16. The solid circles indicate co-exposure of the cells to drug and virus; the solid squares, preincubation of the cells with drug before addition of virus; and the open squares, preincubation of the cells with virus before addition of drug. Example 16 Inactivation of HSV-1 by KY compounds Purified HSV-1 was suspended in RPMI-1640 medium (Gibco Laboratories) containing 2% FCS, penicillin and streptomycin. To aliquots of the suspensions were added control, KY-1, or KY-217 solution, to a final drug concen- tration of 10 μg/ml, and a final virus particle concentra¬ tion of 6 x 10 6 or 6 x 10 s PFU/ml. The suspensions were incubated for 1 hour at 37°C, then diluted serially at 10 fold dilutions to final drug concentrations of 10, 10°, 10' *, 10" 2 , 10" 3 , and 10"* μg/ml drug concentrations. The serially diluted particles were then added to Vero cells for two hours, as in Example 6, and the cells examined for plaques 48 hours later. The number of plaques counted on each of two plates, for each virus and drug concentration, are given in Table 9. Example 17 Binding of KY Compounds to HSV Proteins A. Binding of KY compound to HSV Proteins HSV-1 and HSV-2 viral suspensions from above, each a a concentration of about 5 x 10 7 CFU/ l, were incubated fo 2 hours at 37°C with 5 x 10 5 cpm M C-labeled KY-1 (50μg/ml) . Each viral suspension was divided into two aliquots an solubilized with 0.5% sodium dodecyl sulfate (SDS) , with o without 1% mercaptoethanol. The four solubilized samples were fractionated on 8.5% polyacrylamide gel, and the gels developed by autoradiography, according to standard procedures. The autoradiographs of the four samples are seen in Figure 17A, where the lanes are HSV-1, with (lane A) and without (lane B) mercaptoethanol, and HSV-2, with (lane D) and without (lane E) mercaptoethanol, with the marker proteins in lane C. B. Identification of Binding Proteins HSV-1 and HSV-2 virus suspensions were solubilized with SDS and fractionated on SDS-PAGE as above. Each sample was run in triplicate, corresponding to groups D, B, and C in Figure 17B. The two gels in each group were analyzed by Western blotting as follows: The gels in groups D, B, and C were first reacted with mouse monoclonal antibody specific against HSV glycoprotein gD, gB, and dC, respectively. The antibodies were obtained from Dr. S. Chatterjee from the University of Alabama. The gels were then incubated with alkaline phosphatase-labeled goat anti- mouse antibody, to label the glycoprotein in each group. The glycoprotein with bound antibody was identified by reaction with Rfl 2 in the presence of nitroblue tetrazolium and bromochloroindolephosphate, according to standard methods. The results are shown in Figure 17B. Example 18 Inhibition of HSV Bv Combined Drug Exposure A. Inhibition of HSV-1 HSV-1 particles were obtained from infected cells, as described in Example 7. IX 10 5 MRC cells were plated in each well of 24 well multi-dish in 1 ml RPMI-1640 + 5% FCS + P/S + 0.1% methylcellulose (15 cps). On the second day, after removal of the medium, 200 μl of HSV-1 at 3 pfu/cell was added for two hours at 37°C. After innoculation, the virus was removed and washed with PBS and then 200 μl of (i) a selected concentration of KY-1 alone (up to 50 μg/ml) , (ii) a selected concentration of acyclovir alone (up to 50 μg/ml) ; (iii) a selected concentration of acyclovir (up to 50 μg/ml) plus 25 μg/ml GL-288; or (iv) a selected concentration of acyclovir (up to 50 μg/ml) plus 50 μg/ml GL-288, were added to the MRC-5 cells in culture. After 24 hours incubation at 37 β C, the plates were frozen at -70°C and the titer of viral yield was assayed by plaque formation in Vero cells. The titration was done by 10-fold serial dilution of each sample, innoculated onto Vero cells for 2 hours at 37°C. and then washed with PBS overlayered with 1% methylcellulose plus 2% fetal calf serum. Two days later, the medium was removed. The cells were stained in 0.8% crystal violet in 50% ethanol. The plaques formed were counted and the titer was calculated from the fold of dilutions. The reduction in virus yield, as a function of com- pound concentration, is seen in Figure 19A. B. Inhibition of HSV-l HSV-2 particles were obtained from infected cells, as described in Example 7. Vero cells were infected with serial dilutions of the virus particles plus KY-1 alone. acyclovir alone, or acyclovir plus GL-228 as described i Section A. Inhibition of viral yields was examined as i Section A above. The reduction in virus yield, as a function of com pound concentration, is seen in Figure 19B. Although the invention has been described wit reference to preferred compounds and method of viru inhibition employing the compounds, it will be appreciate that various modification and changes may be made withou departing from the invention.

Claims: IT IS CLAIMED: 1. For use in inhibiting cell infection by an enveloped virus, a pharmaceutical composition comprising a macrocyclic compound composed of aryl ring subunits which are connected by ring-attached bridge linkages which form a continuous chain of connected atoms making up the backbone of the macrocycle, and which contain sulfonic-acid derived substituents on non-backbone atoms of the aryl subunits, and a pharmaceutically acceptable carrier for delivery of the compound by oral, topical, or parenteral routes. 2. The composition of claim 1, wherein the polar sulfonic acid-derived substituents are selected from the group consisting of sulfonic acid, sulfonate salt, sulfinic acid, sulfinate salt, a sulfone, and a sulfonamide. 3. The composition of claim 1, wherein the aryl ring subunits are selected from the group consisting of (a) naphthalene subunits with 1- and 8-position polar groups, and 3- and 6-position sulfonic acid-derived substituents, (b) phenyl subunits with 1-position polar groups, and 4- position sulfonic acid derived groups, and (c) a mixture of (a) and (b) , where the bridge linkages are between the 2 ring-carbon position of one naphthalene or phenyl group, and the 7 ring-carbon group of an adjacent naphthalene group or 5 ring-carbon position of an adjacent phenyl group. 4. The composition of claim 3, wherein the compoun has the form: where 1^ is sulfonic acid, sulfonate salt, sulfinic acid, sulfinate salt, a sulfone, or a sulfonamide, R x is OH, -0, an alkyl or aryl ether, ester, or acid, or a mixtur -thereof, R 4 is >CHR", or >CR", where R is H or carboxyli acid group and n = 4, 6, or 8. 5. The composition of claim 4, wherein R j is an alkyl sulfone or SO j NHR, where NHR is NH j , NHOH or an amino acid. 6. The composition of claim 3, which has the form. where R j is sulfonic acid, sulfonate salt, sulfinic acid, sulfinate salt, a sulfone, or a sulfonamide, R 1 is OH, =0, an alkyl or aryl ether, ester, or acid, or a mixture -thereof, is >CHR", or ≥CR , where R is H or carboxylic acid group and n = 4, 6, or 8. 7. The composition of claim 6, wherein j is an alkyl sulfone or SO^IHR, where NHR is NH j , NHOH or an amino acid. 8. The composition of claim 1, wherein some of the R t groups are =0. 9. The composition of claim 9, for use in oral or parenteral administration, which further includes a protamine sulfate in an amount effective to inhibit anti¬ coagulant effects of the macrocyclic compound. 10. The composition of claim 1, wherein the composi¬ tion further includes an antiviral nucleoside analog compound. 11. The composition of claim 10, for use in treating herpes simplex virus, wherein the nucleoside analog is acyclovir, and the carrier is selected from one of the group: (a) an ointment for topical administration; (b) an ointment or solution for ophthalmic administra¬ tion; (c) an injectable solvent for intravenous administra¬ tion; and (d) a solid carrier for oral administration. 12. The composition of claim 10, for use in human immunodeficiency virus, wherein the nucleoside analog is azidothymidine or dideoxyinosine, and the carrier selected from one of the group: (a) an injectable solvent for intravenous adminis tion; and (b) a solid carrier for oral administration. 13. The composition of claim 10, for use in trea respiratory syncytial virus, wherein the nucleoside an is ribavarin, and the carrier is selected from one of group: (a) a powder or spray for inhalation administrati (b) an injectable solvent for intravenous administ tion; and (c) a solid carrier for oral administration. 14. The composition of claim 10, for use in treat hepatitis B virus, wherein the nucleoside analog is riba rin, and the carrier is selected from one of the group (a) an injectable solvent for intravenous administ tion; and (b) a solid carrier for oral administration. 15. The composition of claim 1, which furt includes protamine sulfate, at a concentration suffici to inhibit anti-coagulant effects associated with presence of macrocyclic compound in the bloodstream. 16. A macrocyclic compound composed of aryl r subunits which are connected by ring-attached bridge li ages which form a continuous chain of connected at making up the backbone of the macrocycle, and which c tain, on non-backbone atoms of the aryl subunits, sulf ic-acid derived substituents selected from the consist of an alkyl sulfone, and a sulfonamide of the form SO j N where NHR is N^, NHOH or an amino acid. 17. The compound of claim 16, wherein the aryl ri subunits are selected from the group consisting of ( naphthalene subunits with 1- and 8-position polar group and 3- and 6-position sulfonic acid-derived substituent (b) phenyl subunits with 1-position polar groups, and 4 position sulfonic acid derived groups, and (c) a mixture o (a) and (b) , where the bridge linkages are between the ring-carbon position of one naphthalene or phenyl group and the 7 ring-carbon group of an adjacent naphthalen group or 5 ring-carbon position of an adjacent pheny group.

Inventors: HWANG KOU M, LIU SU-YING, QI YOU MAO, HWANG, KOU, M., LIU, SU-YING, QI, YOU, MAO
Applicants: GENELABS INC, GENELABS INCORPORATED
Application year: 1992
Main classification: C07C309
IPC codes: A61K31, A61P31, C07C59, C07C65, C07C309, C07C311, C07C313, C07C317, C07C323, C07D257, C07F9