Composition containing dioxabicyclo[3.3.0]octane derivative

Abstract: A food, drink, pharmaceutical, cosmetic composition of food additive comprising a dioxabicyclo[3.3.0]octane derivative represented by the following general formula (I): wherein R¹, R², R³, R⁴, R⁵ and R⁶ independently represent a hydrogen atom or an alkyl group having 1 to 3 carbon atoms, or R¹ and R² and/or R⁴ and R⁵ together form a methylene group or an ethylene group, and n, m and ℓ are 0 or 1; and antioxidant.

Description: The present invention relates to a food or pharmaceutical composition containing a dioxabicyclo[3.3.0]octane derivative and an antioxidant. US-A-4427694 discloses that sesamin is effective for alleviating the symptoms of alcohol intoxication and/or alcohol or tobacco withdrawal, and JP-A-2/138120 discloses that sesaminol and episesaminol are effective for therapeutic or prophylactic treatment of allergic diseases such as bronchial asthma. Nevertheless, compounds which are safe and enhance the activities of dioxabicyclo[3.3.0]octane derivatives are not known, end therefore, there is a desire for a development of novel compositions providing enhanced actions of dioxabicyclo[3.3.0]octane derivatives. Note, the present inventors have proposed various uses of dioxabicyclo[3.3.0]octane derivatives, including inhibition of Δ 5 -desaturase (JP-A-3/27319 and EP-A-387000), improvement of liver functions (Japanese Patent Application No. 2-002345), a lowering of cholesterol levels (Japanese Patent Application No. 2-002637), an inhibition of oncogenesis (Japanese Patent Application No. 2-002818), and a prevention of sickness due to alcohol (Japanese Patent Application No. 3-104016). It is known that sesaminol, and 3 possible stereoisomers thereof, i.e., 2-episesaminol, 6-episesaminol and disesaminol are antioxidant lignan-type compounds (Agr. Biol. Chem. 51(5), 1285-1289, 1987). Sesaminol also is known as a natural antioxidant substance (Agr. Biol. Chem 49(11) 3351-3352 (1985)) US-A-4836987 discloses compounds having lignan backbone and oxygen-containing sidechain on oxygen-containing ring, such as l-sesamin, sesamolin etc. exhibit acceleration of blood clotting action and hemostatic action. JP-A-60/224629 discloses an agent for inhibiting in-vivo formation of peroxidated lipid comprising sesame extract having antioxidant activity. Use of sesamin as insecticide synergest is known. Moreover, as a use of sesamolin, synergestics of pyrethrum insecticides are known (The MERCK INDEX 11th Edition 1989, 1343). The present invention provides pharmaceutical compositions, food and drink, as well as food additives, comprising dioxabicyclo[3.3.0]octane derivatives and a compound which enhances the actions of the dioxabicyclo[3.3.0]octane derivatives. In one aspect the present invention provides a pharmaceutical composition comprising a blend of (a) one or more dioxabicyclo[3.3.0]octane compounds represented by the following general formula (I): in which R 1 , R 2 , R 3 , R 4 , R 5 and R 6 are independently selected from H and C 1-3 alkyl, provided that R 1 and R 2 and/or R 4 and R 5 together may form a methylene group or an ethylene group, and n, m and l are 0 or 1; and (b) an antioxidant which is not a compound of formula (I); the weight ratio (a):(b) in the blend being in the range 1:0.1 to 1:20. Another aspect is a method of making such a composition, comprising blending (a) and (b). A further aspect is a food or drink comprising an artificial blend of components (a) and (b) as set out above. A further aspect is a method of modifying a food or drink containing one or more compounds of formula (I) by adding antioxidant to the food or drink to provide a weight ratio as set out above, and to enhance the in vivo activity of the compound of formula (I). A further aspect is the use of (a) and (b) by blending them in the preparation of a pharmaceutical composition, food, drink, or food or drink additive, to enhance physiological activity of (a). As the dioxabicyclo[3.3.0]octane derivative, in the present invention, sesamin, sesaminol, episesamin, episesaminol, sesamolin, 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane, 2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane or 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyclo[3.3.0]octane can be used. These derivatives can be used alone or in the form of a mixture of two or more thereof. The compound, or extract composed mainly of the compound, may be obtained according to the following procedures. First, an extract composed mainly of the compound of the present invention can be obtained from sesame oil, by a method comprising extracting sesame oil with an organic solvent substantially immiscible with sesame oil and capable of extracting and dissolving the compound of the present invention, and concentrating the extract. As the organic solvent, there can be mentioned, for example, acetone, methylethyl ketone, diethyl ketone, methanol and ethanol. For example, an extract composed mainly of the compound of the present invention can be obtained by mixing sesame oil homogeneously with an organic solvent as mentioned above, allowing the mixture to stand at a low temperature, carrying out a phase separation according to a customary process, and removing the solvent from the solvent fraction by evaporation. More specifically, sesame oil is dissolved in 2 to 10 volumes, preferably 6 to 8 volumes of acetone, and the solution is allowed to stand at -80°C overnight. As a result, the oil component is precipitated and the organic solvent is removed from the obtained filtrate by distillation, whereby an extract composed mainly of the compound of the present invention is obtained. Alternatively, sesame oil is mixed with hot methanol or hot ethanol, the mixture is allowed to stand at room temperature, and the solvent is removed from the solvent fraction to obtain an extract composed mainly of the compound of the present invention. More specifically, sesame oil is mixed with hot methanol (higher than 50°C) or hot ethanol (higher than 50°C) in a volume of 2 to 10 times, preferably 5 to 7 times the volume of the sesame oil, to effect a violent extraction. The phase separation is effected by a phase separation when standing at room temperature or a centrifugal separation according to customary procedures, and the solvent is removed from the solvent fraction by distillation to obtain an extract composed mainly of the compound of the present invention. Furthermore, the supercritical gas extraction can be utilized. The compound of the present invention can be obtained from an extract as mentioned above by treating the extract by a customary method such as column chromatography, high performance liquid chromatography, recrystallization, distillation, or liquid-liquid countercurrent distribution chromatography. More specifically, by using a reversed phase column (5C 18 ) and methanol/water (60/40) as the eluent, the extract is subjected to high performance liquid chromatography, the solvent is removed by distillation, and the obtained crystal is recrystallized from ethanol to obtain the compound used in the present invention, such as sesamin, episesamin, sesaminol or episesaminol. The sesame oil used in the present invention can be either a purified product or a crude product. Furthermore, sesame seeds or sesame lees (defatted sesame seeds having a residual oil content of 8 to 10%) can be used. In this case, sesame seeds or sesame lees are pulverized if necessary, and then subjected to the extraction according to customary procedures using an any solvent, for example , a solvent as mentioned above with respect to the extraction from sesame oil. The extraction residue is separated, and the solvent is removed from the extract by evaporation or the like to obtain an extraction product. The compound used in the present invention, for example, sesamin, sesaminol, episesamin, episesaminol, sesamolin, 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane, 2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane or 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyclo[3.3.0]octane, can be obtained from a sesame seed extract, a sesame lee extract or a crude sesame oil extract according to the same procedures as described above. Moreover, the compound used in the present invention can be obtained from a by-product formed in the sesame oil-preparing process. Note, sesamin obtained from Piper longum L exhibits the same effects as those provided by sesame seeds, sesame lee and sesame oil. Moreover, optically active isomers of the above-compounds also may be used in the present invention. The process for the purification of the compound used in the present invention and the process for obtaining the extract are not limited to those mentioned above, and the compound used in the present invention and the extract composed mainly of the compound of the present invention are not limited to those obtained from sesame oil, sesame lees and sesame seeds, but as is apparent to persons with ordinary skill in the art, all e.g. natural substances containing the compound used in the present invention can be used. For example, there can be mentioned Acanthopanax ghacilistylus , Asari Herba Cum Redice, Ginkgo - biloba and Piper longum L. The following processes can be adopted for the synthesis of the compound of the present invention. For example, sesamin and episesamin can be synthesized according to the process of Beroza et al. [J. Am. Chem. Soc., 78 , 1242 (1956)). Pinoresinol [in the general formula (I), R 1 and R 4 represent H, R 2 and R 5 represent CH 3 , and n, m and ℓ are zero] can be synthesized according to the process of Freundenberg et al. [Chem. Bar., 86 , 1157 (1953)). Furthermore, syringaresinol [in the general formula (I), R 1 and R 4 represent H, R 2 , R 3 , R 5 and R 6 represents CH 3 , n is zero, and each of m and ℓ is 1] can be synthesized according to the process of Freundenberg et al [Chem. Ber., 88 , 16 (1955)]. The compound used in the present invention also can be used in the form of a glycoside. Furthermore, compounds used in the present invention can be used alone or in combination. As antioxidants in the present invention, there are included natural antioxidants such as tocopherols, flavone derivatives, phyllodulcins, kojic acid, gallic acid derivatives, catechins, fukinolic acid, gossypol, pyrazine derivatives, sesamol, guaiacol, guaiac resin, p-cumaric acid, nordihydroguaiaretic acid, sterols, terpenes, purine or pyrimidine bases, carotenoides and the like, as well an synthetic antioxidants such as butylhydroxyanisol (BHA), butylhydroxytoluene (BHT), mono-tert.-butyl hydroquinone (TBHQ), 4-hydroxymethyl-2,6-di-tert.-butylphenol (HMBP). The tocopherols include α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, ε-tocopherol, ξ-tocopherol, η-tocopherol, and tocopherol esters such as tocopherol acetates, and the carotenoids includes β-carotene, canxanthine, astaxanthine. As actions of dioxabicyclo[3.3.0]octane derivatives, there are known Δ5-desaturase inhibition, cholesterol uptake inhibition, cholesterol synthesis inhibition, cholesterol metabolism control, prevention or treatment of fatty liver, liver disorders caused by alcohol, treatment of liver disorders caused by chemicals, acceleration of alcohol methaholism, decrease of serum cholesterol or neutral fat, depression of parasympathetic nervous functions when eating or drinking, depression of an increase of temperature of face surface by drinking alcohol and acceleration of returning to normal temperature, and inhibition of breast cancer. As actions of sesamin, there are known acceleration of blood clotting and hemostatic action, enhancement of insecticidal activity, psychotropically effective action and the like. Moreover, it is known that sesamolin exhibits acceleration of blood clotting and hemostatic action. It is known that sesaminol and stereoisomers thereof, sesamobinol (2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyelo[3.3.0]octane), 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,4-dioxabicyclo[3.3.0]octane, pinoresinol (2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane have antioxidant activity. As described above, although dioxabicyclo[3.3.0]octane derivatives have various actions, the trend of actions is as follows: control of in-vivo fatty acid metabolism, increase of dihomo-γ-linolenic acid and eicosanoid thereof, repression of inflammation and thrombus, lowering of blood pressure, improvement of liver function, inhibition of oncogenesis of colon cancer and breast cancer, prevention of sick from drink, alleviation of alcoholism, acceleration of blood clotting, prevention and treatment of cardio-vascular diseases caused by high blood cholesterol, and prevention of accumulation of cholesterol in various parts of the circular system; and in case of those having antioxidant action, prevention of oxidation of oil or fat per se., foods containing oil or fat and cosmetics, inhibition of active oxygen radical formed by peroxidation of cytoplasmic lipid and cellular components or by metabolism to prevent cell injury. According to the present invention, the above-mentioned actions are enhanced by combination with antioxidant such as tocopherol, and a smaller amount of the dioxabicyclo[3.3.0]octane derivative provides significant activities. According to the present invention, although a ratio of dioxabicyclo[3.3.0]octane and antioxidant is not critical, particularly for an enhancing of the dioxabicyclo[3.3.0]octane derivatives actions, especially the cholesterol-lowering action, the ratio of dioxabicyclo[3.3.0]octane/antioxidant is preferably between 0.001 and 1000, more preferably 0.01 to 20, more preferably 0.1 to 20, most preferably 0.2 to 10. The present compositions are provided as pharmaceutical compositions, cosmetic, food or drinks, or food additives. The pharmaceutical compositions may be administered exterally, or parenterally, for example, intramuscularly, subcutaneously, or intravenously. The dosage varies depending on the purpose of the administration and the conditions of subjects receiving the composition, but to lower the cholesterol level, a dose for an oral administration is generally 1 to 100 mg/day, and a dose for a parenteral administration is 0.1 to 20 mg/day, for an adult human. To prepare an injectable composition, a medical solubilizing agent such as nonionic surfactant can be used. More particularly, the present pharmaceutical composition can be prepared, for example, by dissolving a compound of the present invention in 80 volume of POE(60)-hardened castor oil, or nonionic surfactant such as POE sorbitan mono oleate with heating, and diluting the resulting mixture with a physiological saline. Optionally, an isotonic agent, stabilizer, a preservative, an analgesic and the like may be added. Moreover, if necessary, emulsions capsules, powders, granules, tablets and the like may be prepared. The present invention further provides food additives comprising a dioxabicyclo[3.3.0]octane derivatives and antioxidant. The addition of the additive is useful because most foods do not contain the same or contain only a very little amount thereof. The present invention still further relates to food or drink to which dioxabicyclo[3.3.0]octane derivatives and/or an antioxidant is added. Since most food and drink does not contain or contains in only a very small amount, both of the above ingredients, then the present food or drink can be produced by adding both ingredients to such a food or drink. Nevertheless, some food already contains a sufficient amount of dioxabicyclo[3.3.0]octane derivative, such as sesamin, and thus it is sufficient to add only an antioxidant for the production of the present food and drink. Since dioxabicyclo[3.3.0]octane derivatives, which are active ingredients of the present compositions, are compounds or analogues thereof found in conventional food, they are advantageous from a safety point of view: Moreover, if antioxidants, able to enhance the actions of dioxabicyclo[3.3.0]octane derivatives are selected from those conventionally used as antioxidants, they are harmless and have no affect on the taste of the food or drink. The food of the present invention, and food to which the present food additives are added is not limited, but considering the cholesterol level-lowering action, food containing fat or oil may be considered. For example, there may be mentioned natural food containing fat or oil, such as meat, fish, nuts and the like; food to which fat or oil is added during the cooking thereof, such as Chinese food, Chinese noodle, soup and the like; food cooked using fat or oil as a heating medium, such as Japanese Tempura, fried food, Chinese fried rice, doughnuts, sugar-coated fries and the like; fatty food such as butter, margarine, mayonnaise, salad dressing, chocolate, instant noodles, caramel, biscuits, ice cream and the like; and food sprayed or coated with a fat or oil at finish, etc. Since the addition of the present composition to oil or fat is easy, the present composition is preferably added to fat or oil. Nevertheless, the food to which the present composition is added is not limited, and the present composition may be added any food to thereby enhance the dioxabicyclo[3.3.0]octane derivative action of such food. For food containing the present composition, although the amounts of dioxabicyclo[3.3.0]octane derivative and antioxidant are not critical, the dioxabicyclo[3.3.0]octane derivatives are preferably used alone or as a mixture in an amount of at least 0.0001% by weight, more preferably at least 0.001% by weight in total, on the basis of the weight of the food; and antioxidants are preferably added alone or as a mixture in an amount of at least 0.00001% by weight, more preferably at least 0.0001% by weight in total, on the basis of the weight of the food. Where an extract containing dioxabicyclo[3.3.0]octane derivatives is used, the extract is preferably used in an amount of at least 0.0004% by weight, more preferably at least 0.004% by weight, on the basis of the weight of the food. Since dioxabicyclo[3.3.0]octane derivatives, an active ingredient of the present composition, are compounds present in conventional food or analogues thereof, then the food containing dioxabicyclo[3.3.0]octane derivatives potentially should have the activities of dioxabicyclo[3.3.0]octane derivatives. In actuality, however, the food does not sufficiently exhibit the actions of dioxabicyclo[3.3.0]octane derivatives, because the content thereof is too low. Accordingly, in one embodiment of the present invention, an antioxidant of the present invention is added to a food containing dioxabicyclo[3.3.0]octane derivative in an amount of 0.001 to 1000 parts by weight, preferably 0.01 to 20 parts by weight, more preferably 0.1 to 20 parts by weight, most preferably 0.2 to 10 parts by weight, relative to one part by weight of dioxabicyclo[3.3.0]octane derivative contained therein, to enhance the actions of dioxabicyclo[3.3.0]octane derivatives, especially the cholesterol level lowering action. Where food or drink containing dioxabicyclo[3.3.0]octane derivative also contains an antioxidant, which enhances the actions of the dioxabicyclo[3.3.0]octane derivative, the same or a different antioxidant can be further added to the food or drink in a total amount of up to 1000 parts by weight, preferably up to 100 parts by weight, more preferably up to 20 parts by weight, most preferably up to 10 parts by weight, relative to one part by weight of the dioxabicyclo[3.3.0]octane derivative, to obtain food or drink having further enhanced actions, such as a cholesterol level lowering action, of the dioxabicyclo[3.3.0]octane derivative. Where tocopherol is used as an antioxidant, for example, where food or drink containing the dioxabicyclo[3.3.0]octane derivative does not contain an antioxidant, the tocopherol may be added to the food or drink in an amount of 0.01 to 100 part by weight, preferably 0.1 to 20 parts by weight, most preferably 0.2 to 10 parts by weight, relative to one part by weight dioxabicyclo[3.3.0]octane derivative, to enhance the actions, such as the cholesterol level lowering action, of the dioxabicyclo[3.3.0]octane derivative. As food containing dioxabicyclo[3.3.0]octane derivates, there can be mentioned sesame, washed sesame, peeled sesame, roasted sesame, roasted and peeled sesame, sesame paste, milled sesame, kneaded sesame, sesame curd, sesame salad oil, roasted sesame oil, sesame powder and the like. Note, food to which an antioxidant is added is not limited to sesame-based food, and the present antioxidants may be added to any food containing dioxabicyclo[3.3.0]octane derivatives to thereby enhance the actions of the dioxabicyclo[3.3.0]octane derivative. According to the present invention a combination of a dioxabicyclo[3.3.1]octane derivative and antioxidant provide a synergistic action, such as synergistic lowering of cholesterol level. Examples Next, the present invention is further described in the following Examples. Example 1. First, 36 male SD rats, 5 weeks old, were prefed with a standard feed (solid CE-2, Nippon Clea) for one week, and then were divided into 6 groups each consisting of 6 animals. The animals were fed for two weeks with a cholesterol-enriched feed comprising 20% casein, 10% powdered beef tallow, 56.75% granule sugar, 4% cellulose, 1% cholesterol, 0.25% cholic acid, 1% vitamin mixture (AIN-TM) and 7% mineral mixture (TIN-TM), or experimental feed supplemented with tocopherol acetate and/or sesamin, in different amounts. The experimental groups were set as follows: 1. Cholesterol feed 2. Cholesterol feed + 1.0% tocopherol acetate 3. Cholesterol feed + 0.05% sesamin 4. Cholesterol feed + 0.05% sesamin + 1.0% tocopherol acetate 5. Cholesterol feed + 0.2% sesamin 6. Cholesterol feed + 0.2% sesamin + 1.0% tocopherol acetate The above-mentioned tocopherol was DL-α-tocopherol acetate (Nacalai Tesque), and the above-mentioned "sesamin" means a mixture of sesamin and episesamin (sesamin 55.2%, episesamin 44.4%, purity 99.6%) prepared according to a procedure described in JP-A-3/27319. (EP-A-387000). After being fed for 2 weeks, the animals were starved, and blood samples were taken. The total cholesterol, HDL-cholesterol, triglycenide phospholipid, GOT and GPT levels in the sera were measured using a biochemical automatic analyzer (Hitachi Model 7050). The LDL-cholesterol value was obtained by calculation. The results are shown in Table 1. An increase of total serum cholesterol value provided by the cholesterol feed was inhibited in a dose-dependent manner, and the inhibition was remarkably enhanced by the addition of tocopherol. In the animal group to which tocopherol alone was provided, the total serum cholesterol level was not significantly changed, revealing that tocopherol enhances the action of sesamin in lowering the cholesterol level. Example 2. First, 27 SD rats, 5 seeks old, were prefed with a standard feed (solid CE-2, Nippon Clea) for one week, and then were divided into 4 groups consisting of 6 or 9 animals. According to a similar procedure as described in Example 1, the animals were fed with a cholesterol feed, or with a cholesterol feed supplemented with tocopherol acetate and/or sesamin, for 2 weeks. The experimental groups were as follows: 1. Cholesterol feed 2. Cholesterol feed + 0.2% sesamin 3. Cholesterol feed + 0.2% sesamin + 0.2% tocopherol acetate 4. Cholesterol feed + 0.2% sesamin + 1.0% tocopherol acetate The above-mentioned tocopherol and sesamin were the same as described in Example 1. After being fed for 2 weeks, the animals were starved for 17 hours, and then blood samples were taken. The total cholesterol, HDL-cholesterol, triglyceride, phospholipid, GOT, and GPT levels in the serum were measured using an automatic biochemical analyzer. The LDL-cholesterol value was calculated. The results are shown in Table 2. It was found that tocopherol enhanced the actions of sesamin in a dose-dependent manner, thus revealing the usefulness of the present composition. Example 3. Sesaminol (Compound A) prepared from purified sesame oil according to Japanese Unexamined Patent Publication No. 1-243992, 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (Compound C) prepared by extracting sesame seeds with acetone, 2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (Compound D), or 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyclo[3.3.0]octane (Compound E) were used in the following experiments. First, 42 SD rats, 5 weeks old, were prefed with a standard feed (solid E-2, Nippon Clea), and were then divided into 7 groups each consisting of 6 animals. Next, the animals were fed with the cholesterol feed as described in Example 1, or an experimental feed supplemented with tocopherol acetate and/or Compound A, B, C, D or E, for 2 weeks. The experimental groups were as follows: 1. Cholesterol feed 2. Cholesterol feed + 0.2% sesamin 3. Cholesterol feed + 0.2% Compound A + 1.0% tocopherol acetate 4. Cholesterol feed + 0.2% Compound B + 1.0% tocopherol acetate 5. Cholesterol feed + 0.2% Compound C + 1.0% tocopherol acetate 6. Cholesterol feed + 0.2% Compound D + 1.0% tocopherol acetate 7. Cholesterol feed + 0.2% Compound E + 1.0% tocopherol acetate The above-mentioned tocopherol acetate was the same as used in Example 1. After being fed two weeks, the animals were starved for 17 hours, and then blood samples were taken. The total cholesterol level was measured using an automatic biochemical analyzer, and as a result, it was confirmed that the up-take of tocopherol acetate significantly enhances the action of each compound in lowering the cholesterol level. Although the total cholesterol value in the serum of the first group was increased to 419±153 mg/dl by the cholesterol feed, the administration of sesamin to the second group reduced the cholesterol value to 361±128 mg/dl. For the groups 3, 4, 5, 6 and 7, wherein Compounds A, B, C, D and E with tocophenol acetate were additionally administered, the total cholesterol values were 198±33, 204±42, 186±37, 211±61 and 218±57 mg/dl respectively, revealing that the Compound A, B, C, D or E used in combination with tocophenol further inhibited the increase of the total cholesterol level value. Example 4. First, 100 g of butter fat prepared by eliminating butter milk during the production of butter by the stirring operation (churning) was mixed with 1.2 g of a mixture of sesamin and episesamin used in Example 1 and 1.2 g of tocopherol acetate, and the kneading operation (working) was carried out to obtain a homogeneous cholesterol level lowering butter containing the present composition. Formulation Example 1. First, 0.25 g of sesamin and 0.25 g of tocopherol acetate were mixed with 20.5 g of silicic anhydride, and 79 g of corn starch was added thereto, followed by a further mixing. Then to the mixture was added 100 ml of 10% hydroxypropylcellulose ethanol solution, the mixture was kneaded and extruded, and the resulting granules were dried. Formulation Example 2. First, 3.5 g of sesamin and 0.5 g of tocopherol acetate were mixed with 20 g of silicic anhydride, and to the mixture were then added 10 g of microcrystalline cellulose, 3.0 g of magnesium stearated, and 60 g of lactose. The mixture was pressed to form tablet having a diameter of 7 mm and a weight of 100 mg by using a single-shot tableting machine. Formulation Example 3. First, 1.25 g of sesamin and 1.25 g tocopherol acetate were dissolved in 200 g of a nonionic surface active agent TO-1014 (Nikko Chemicals) while heating at 122°C, and 4.7975 ℓ of sterilized physiological saline was added thereto. The mixture was then thoroughly stirred, aseptically filled in vials, which were then closed, to obtain an injectable formulation.

Claims: Claims for the following Contracting States : AT, BE, CH, LI, DE, DK, FR, GB, IT, NL, PT, SE A pharmaceutical composition comprising a blend of (a) one or more dioxabicyclo[3.3.0]octane compounds represented by the following general formula (I): in which R 1 , R 2 , R 3 , R 4 , R 5 and R 6 are independently selected from H and C 1-3 alkyl, provided that R 1 and R 2 and/or R 4 and R 5 together may form a methylene group or an ethylene group, and n, m and l are 0 or 1; and (b) an antioxidant which is not a compound of formula (I); the weight ratio (a):(b) in the blend being in the range 1:0.1 to 1:20. A pharmaceutical composition according to claim 1, wherein the dioxabicyclo[3.3.0]octane compound is selected from sesamin, sesaminol, episesamin, episesaminol, sesamolin, 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane, 2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane and 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyclo[3.3.0]octane. A pharmaceutical composition according to claim 1 in which the compound of formula (I) is sesamin, episesamin or sesamolin. A pharmaceutical composition according to any one of the preceding claims in which the antioxidant is selected from tocopherols, flavone derivatives, phyllodulcins, kojic acid, gallic acid derivatives, catechins, fukinolic acid, gossypol, pyrazine derivatives, sesamol, guaiacol, guaiac resin, p-coumaric acid, nordihydroguaiaretic acid, sterols, terpenes, purine and pyrimidine bases, carotenoides, butylhydroxyanisol (BHA), butylhydroxytoluene (BHT), mono-tert.-butyl hydroquinone (TBHQ), and 4-hydroxymethyl-2,6-di-tert-butylphenol (HMBP). A pharmaceutical composition according to claim 4 in which the antioxidant is a tocopherol. A pharmaceutical composition according to any one of the preceding claims in which the weight ratio (a):(b) is in the range 1:0.2 to 1:10 A food additive comprising an artificial blend of components (a) and (b) as defined in any one of claims 1 to 6. A food or drink comprising an artificial blend of components (a) and (b) as defined in any one of claims 1 to 6. A food or drink according to claim 8 containing native compound of formula (I), to which antioxidant (b) has been added to give proportions (a):(b) in the stated range. A method of modifying a food or drink containing one or more dioxabicyclo[3.3.0]octane compounds as defined in claim 1, to enhance the in vivo activity of said compound(s) in the consumer of the food or drink, comprising adding antioxidant to the food or drink to give a weight ratio of said compound(s) to antioxidant in said food or drink in the range from 1:0.1 to 1:20, the antioxidant not being of formula (I). A method according to claim 10 in which said weight ratio is from 1:0.2 to 1:10. A method according to claim 10 or 11 in which said compound(s) is/are as defined in claim 2 or claim 3. A method according to claim 10, 11 or 12 in which said antioxidant is as defined in claim 4 or claim 5. Use of (a) one or more dioxabicyclo[3.3.0]octane compounds represented by the following general formula (I): in which R 1 , R 2 , R 3 , R 4 , R 5 and R 6 are independently selected from H and C 1-3 alkyl, provided that R 1 and R 2 and/or R 4 and R 5 together may form a methylene group or an ethylene group, and n, m and l are 0 or 1; and (b) an antioxidant which is not a compound of formula (I); by blending the antioxidant with the compound(s) in the preparation of a pharmaceutical composition, food, drink, or food or drink additive, to enhance physiological activity of the compound(s). Use according to claim 14 in which the weight ratio (a):(b) of the compound(s) to the antioxidant is in the range from 1:0.001 to 1:1000. Use according to claim 15 in which the weight ratio (a):(b) is in the range from 1:0.01 to 1:20. Use according to any one of claims 14 to 16 in which the physiological activity is to inhibit uptake of cholesterol, inhibit synthesis of cholesterol or control metabolism of cholesterol. Use according to any one of claims 14 to 16 in which the physiological activity is to treat the effects of alcohol intake. Use according to any one of claims 14 to 18 in which the compound(s) is/are as defined in claim 2 or claim 3. Use according to any one of claims 14 to 19 in which the antioxidant is as defined in claim 4 or claim 5. Claims for the following Contracting State : ES A method of making a pharmaceutical composition, comprising blending (a) one or more dioxabicyclo[3.3.0]octane compounds represented by the following general formula (I): in which R 1 , R 2 , R 3 , R 4 , R 5 and R 6 are independently selected from H and C 1-3 alkyl, provided that R 1 and R 2 and/or R 4 and R 5 together may form a methylene group or an ethylene group, and n, m and l are 0 or 1; and (b) an antioxidant which is not a compound of formula (I); the weight ratio (a):(b) in the blend being in the range 1:0.1 to 1:20. A method according to claim 1, wherein the dioxabicyclo[3.3.0]octane compound is selected from sesamin, sesaminol, episesamin, episesaminol, sesamolin, 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane, 2,6-bis-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane and 2-(3,4-methylenedioxyphenyl)-6-(3-methoxy-4-hydroxyphenoxy)-3,7-dioxabicyclo[3.3.0]octane. A method according to claim 1 in which the compound of formula (I) is sesamin, episesamin or sesamolin. A method according to any one of the preceding claims in which the antioxidant is selected from tocopherols, flavone derivatives, phyllodulcins, kojic acid, gallic acid derivatives, catechins, fukinolic acid, gossypol, pyrazine derivatives, sesamol, guaiacol, guaiac resin, p-coumaric acid, nordihydroguaiaretic acid, sterols, terpenes, purine and pyrimidine bases, carotenoides, butylhydroxyanisol (BHA), butylhydroxytoluene (BHT), mono-tert.-butyl hydroquinone (TBHQ), and 4-hydroxymethyl-2,6-di-tert-butylphenol (HMBP). A method according to claim 4 in which the antioxidant is a tocopherol. A method according to any one of the preceding claims in which the weight ratio (a):(b) is in the range 1:0.2 to 1:10 A food or drink comprising an artificial blend of components (a) and (b) as defined in any one of claims 1 to 6. A food or drink according to claim 7 containing native compound of formula (I), to which antioxidant (b) has been added to give proportions (a):(b) in the stated range. A method of modifying a food or drink containing one or more dioxabicyclo[3.3.0]octane compounds as defined in claim 1, to enhance the in vivo activity of said compound(s) in the consumer of the food or drink, comprising adding antioxidant to the food or drink to give a weight ratio of said compound(s) to antioxidant in said food or drink in the range from 1:0.1 to 1:20, the antioxidant not being of formula (I). A method according to claim 9 in which said weight ratio is from 1:0.2 to 1:10. A method according to claim 9 or 10 in which said compound(s) is/are as defined in claim 2 or claim 3. A method according to claim 9, 10 or 11 in which said antioxidant is as defined in claim 4 or claim 5.

Inventors: AKIMOTO KENGO, KITAGAWA YOSHINORI, NAKABAYASHI AYAKO, SUGANO MICHIHIRO, AKIMOTO, KENGO, KITAGAWA, YOSHINORI, NAKABAYASHI, AYAKO, SUGANO, MICHIHIRO
Applicants: SUNTORY LTD, SUNTORY LIMITED
Application year: 1992
Main classification: A61K31
IPC codes: A61K31, A61K47, A61K36, A23L1, A61P43, A23L3, A61P3