LIPID DERIVATIVES OF PHOSPHONOACIDS FOR LIPOSOMAL INCORPORATION AND METHOD OF USE

Abstract: Lipid-containing prodrugs are provided for treating viral infections due to herpes, influenza, hepatitis B, Epstein-Barr, and varicella-zoster viruses, as well as cytomegalovirus and derivatives of antiviral agents. The compounds comprise phosphonoacids having antiviral activity which are linked, either through the phosphate group or carboxyl group of the phosphonoacid, to one of a selected group of lipids. Phosphonoacetic acid and phosphonoformic acid are thus linked to phospholipids, glycerolipids, sphingolipids, glycolipids, or fatty acids. The compounds persist, after intracellular hydrolysis, as the antiviral phosphonoacids. The lipid prodrugs are effective in improving the efficacy of antiviral phosphonoacids by prolonging their antiviral activity following administration.

Description: LIPID DERIVATIVES OF PHOSPHONOACIDS FOR LIPOSOMAL INCORPORATION AND METHOD OF USE Background of the Invention The present invention relates generally to the treatment of viral infections using lipid derivatives of antiviral compounds. More particularly, the present invention relates to lipid derivatives of antiviral phosphonoacids and their use. The lipid derived compounds can be integrated into the structure of liposomes, thereby forming a more stable liposomal complex which can deliver greater amounts of these compounds to target cells with less toxicity. The publications and other reference materials referred to herein are hereby incorporated by reference, and are listed for convenience in the bibliography appended at the end of this specification. There has been a great deal of interest in recent years in developing agents to treat viral infections. In the past the most significant viral diseases were those caused by viruses of the herpes and influenza groups of viruses as well as those viruses causing hepatitis. In the past decade, infections with human immunodeficiency retrovirus (HIV) have become a major public health problem. Effective antiviral agents are those that interfere with the replication or transcription of viral genetic information while not inhibiting the normal functions of the host cell. Phosphonoacetic acid (PAA) and phosphonoformic acid (PFA or Foscarnet) , having the following structures: O O HOOCCH, OH HOOC PII OH (PAA) (PFA) have been shown to have good antiviral activity against herpes simplex viruses types 1 and 2 (1) , as well as against influenza viruses, hepatitis virus B, and retrovirus infections (2) . Acquired immunodeficiency syndrome (AIDS) is caused by the human immunodeficiency virus (HIV) . HIV is a retroviru which infects cells bearing the CD4 (T4) surface antigen, suc as CD4+ helper lymphocytes, CD4+ monocytes and macrophages an certain other CD4+ cell types. The HIV infection of CD4 lymphocytes results in cytolysis and cell death whic contributes to the immunodeficiency of AIDS; however, CD4 monocytes and macrophages may not be greatly harmed by th virus. Viral replication in these cells appears to be mor prolonged and less cytotoxic than in lymphocytes, and as result, monocytes and macrophages represent importan reservoirs of HIV infection. It has recently been discovere that macrophages may serve as reservoirs of HIV infection eve in certain AIDS patients who test negative for the presence o HIV antibodies. No effective cure is available for AIDS although nucleosides analogues, particularly th dideoxynucleosides have been shown to prolong life and t reduce the incidence of certain fatal infections associate with AIDS. Dideoxynucleoside analogues are the most potent agent currently known for treating AIDS, but these therapies are no entirely satisfactory. In a recent human clinical tria using AZT, serious toxicity was noted, evidenced by anemi (24%) and granulocytopenia (16%) (3,4). Certain monocyte derived macrophages, when infected with some strains of HIV have been found to be resistant to treatment wit dideoxycytidine, azidothymidine, and other dideoxynucleoside in vitro as shown by Richman, et al. (5) . The resistance ma be due in part to the low levels of dideoxynucleoside kinas which result in a reduced ability to phosphorylate AZT, ddC o ddA. Phosphonoformate (PFA) may provide an effectiv alternative therapy to nucleoside analogues. PFA inhibits broad range of DNA polymerases as well as the RNA polymeras of influenza virus. PFA also inhibits the revers transcriptase (RT) of HIV and other retroviruses a concentrations below 1 μM. Since the DNA polymerases are muc less sensitive to PFA than the reverse transcriptases, th possibility exists that this drug may have a good therapeuti ratio for use in HIV infection. The phosphonoacids PFA an PAA may also supplement therapy using antiviral nucleosides Lambert (6) has found that when PFA or PAA are coupled wit certain antiviral nucleosides, particularly 5-bromo-2' deoxyuridine (BUdR) , the antiviral activity of the couple nucleoside against herpes simplex viruses is greater than tha of the parent nucleoside. Efforts to increase the effectiveness of both antivira nucleosides analogs and the phosphonoacids include associatio of these agents with lipids. Attempts have been made to improve the therapeuti effectiveness of nucleoside analogues, such a arabinofuranosylcytosine (ara-C) and arabinofuranosyladenin (ara-A) as chemotherapeutic agents in the treatment of variou types of cancer, by chemically linking them to phospholipid in order to enhance their catabolic stability (7) . Thes phospholipid-derived agents showed a decreased toxicity an increased stability over the nucleoside analogues alone However, they also exhibited poor cellular uptake (6) and poo drug absorption (8) . Another approach to increase the effectiveness o antiviral agents comprises encapsulation within liposomes t facilitate their delivery to cells. Liposomes are lipi vesicles which can be formed according to the method of Ale Bangham. Bangham and coworkers discovered in 1965 that drie films of phosphatidylcholine spontaneously formed close bi olecular leaflet vesicles upon hydration (9) . One of th most effective applications of liposomes in medicine is as carrier to deliver therapeutic agents to target organs. Th agents are encapsulated during the process of liposom formation and released in vivo after liposomes are taken up b cells. Liposomes thereby provide a means of delivering highe concentrations of therapeutic agents to target organs Further, since liposomal delivery focuses therapy at the sit of liposome uptake, it reduces toxic side effects. Liposomal incorporation has been shown to provide a more effective way of delivering antiparasitic compounds which not only increases the potency of the dose but prolongs its efficacy and decreases its toxicity. For example, liposomal antimonial drugs are several hundred-fold more effective than the free drug in treating leishmaniasis as shown independently by Black and Watson (10) and Alving, et al. (11). Liposome- entrapped amphotericin B appears to be more effective than the free drug in treating immunosuppressed patients with systemic fungal disease (12) . Other uses for liposome encapsulation include restriction of doxorubicin toxicity (13) and diminution of aminoglycoside toxicity (14) . PFA has been found to inhibit HIV-1 replication in several in vitro systems at concentrations which are attainable in patients. However, the low degree to which PFA enters cells causes much higher levels to be required than that found to be effective in cell free systems with HIV RT (15) . Also, PFA has toxic side effects and is known to accumulate in bone because of its similarity to pyrophosphate. Phosphonoacetic acid (PAA) , which has antiviral activity similar to that of PFA, appears to have an affinity for bone that may preclude its use in humans (6) . Attempts have been made to increase the intracellular antiviral efficacy of the phosphonoacids by encapsulating them into liposomes. Szoka, F. and Chu, C. (16) found that liposomal delivery enhanced the cellular uptake and viral inhibitory activity of both PFA and PAA. Liposomal encapsulation also decreased the cytopathic effect of PFA; however, the cytopathic effect of liposomal PAA as compared to the free drug appeared to be increased. As previously mentioned, it is now thought that macrophages are an important reservoir of HIV infection (17, 18) . Macrophages are also a primary site of liposome uptake (19, 20). Accordingly, it would be desirable to utilize liposomes to enhance the effectiveness of antiviral phosphonoacid and antiviral phosphonoacid esters of antiviral nucleosides in treating AIDS and other viral infections. Clearly, it would be useful to have more effective ways of delivering large amounts of effective antiviral phosphonoformate compounds to macrophages infected with HIV or other viruses and to other cells having viral infections. Co-pending applications, U. S. Serial Noε. 216,412 and 319,485 (21, 22) disclose lipid derivatives of nucleoside analogues which are capable of being incorporated into the structure of liposomes so as to further improve therapy comprising liposomal delivery of these agents. In order to use phosphonoacid antivirals more effectively, it is desirable to synthesize lipid prodrugs of the agents. It is therefore an object of the invention to provide methods for producing phosphonoacid lipid derivatives which can be incorporated into stable liposomal form. The methods disclosed here apply not only to the use of lipid derivatives of phosphonoacids in the treatment of AIDS and other retroviral diseases, but also to their use in the treatment of diseases caused by other viruses, such as influenza, herpes simplex virus (HSV) , human herpes virus 6, cytomegalovirus (CMV) , hepatitis B virus, Epstein-Barr virus (EBV) , and varicella zoster virus (VZV) . BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 demonstrates the effect of a composition of the present invention on plaque formation in HIV-infected cells. SUMMARY OF THE INVENTION The invention provides a composition having antiviral properties, comprising an antiviral phosphonoacid having a lipid moiety linked thereto. In a preferred embodiment, the phosphonoacid is phosphonoformic acid, and in another embodiment, the phosphonoacid is phosphonoacetic acid. The phosphonoacid can be attached to the lipid group through either a phosphate ester or carboxyester linkage. The invention also includes phosphonoacids linked to lipids through diphosphate groups. According to one embodiment of the invention, the lipid moiety attached to the phosphonoacid is a diacyl- or dialkylglycerol or a l-acyl-2-O-alkyl glycerol, or a 1-0- alkyl-2-acylglycerol; in other embodiments, the lipid moiet is a monoacyl- or monoalkylglycerol or a fatty acid, phospholipid, or a more complex lipid moiety, such as, fo example, a sphingosine, a ceramide, cardiolipin, o bis(diacylglycero)phosphate. The lipid moiety may compris from 1 to 4 long chain aliphatic groups, each comprising fro 2 to 24 carbon atoms, which may be unsaturated, containin from 1 to 6 double bonds. The aliphatic groups of glycerol may be attached to the constituent glycerol unit by ester ether, or vinyl ether bonds. The aliphatic groups of lipid having more than one such group may be the same or differen in structure. Preferred phosphonoacid lipid derivatives are 1-O-alkyl sn-glycero-3-phosphonoacids. l-0-hexadecyl-s/2-glycero-3 phosphonoacids are particularly preferred. Other preferre embodiments are l-O-alkyl-s/j-glycero-3-oxycarbonyl phosphonoacids. According to another aspect of the invention there ar provided lipid-rich derivatives of phosphonoacids of th general formula (VI) and including bisdiacylglycerol phosphat phosphonoacids and diphosphatidylglycerol derivatives o phosphonoacids. The invention further comprises a liposome formed a least in part from any of the compositions of the invention Another aspect of the present invention is a method fo preparing a suspension of liposomes for use in treating vira infections in a mammal, comprising providing an lipophili antiviral agent selected from the group consisting of phosphonoacid linked to a lipid moiety; combining th lipophilic antiviral agent and a pharmacologically acceptabl aqueous solvent to form a mixture; and forming liposomes fro the lipophilic antiviral agent. The invention further comprises methods for treatin viral infection in a mammal, comprising administering a effective amount of any one of the compositions of th invention. In a preferred embodiment, the mammal is a human and the virus is the retrovirus HIV. In particularly preferred embodiments, a method of the invention is used in combination with the administration of AZT or other antiretroviral analogues. The methods may include avoiding or overcoming resistance to AZT or other antiviral analogues by administering the analogues in the form of one of the compositions of the invention. The lipophilic nature of these compounds provides advantages over the use of the phosphonoacids alone, by prolonging their persistence in vivo following administration. The lipid-phosphonoacids can also be incorporated into the lamellar structure of liposomes when combined with similar lipophilic molecules. In the form of liposomes, these antiviral molecules are preferentially taken up by macrophages and monocytes, cells that have been found to harbor the target human immunodeficiency virus (HIV) . Additional site specificity can be incorporated into the liposomes by the inclusion of ligands, such as monoclonal antibodies or other peptides or proteins that bind to viral proteins. These prodrugs have been found to prevent viral replication in HIV infections that have become resistant to therapy with conventional forms of the antiretroviral agents. DETAILED DESCRIPTION OF THE INVENTION The invention provides synthesized lipid derivatives of phosphonoacids which can be incorporated into the lipid bilayer of liposomes. These derivatives are converted into antiviral phosphonoacids by constituent metabolic processes, and they accordingly have antiviral effects in vivo and in vitro. A phosphonoacid has the general structure O II HO—P—O—(CH 2 ) n —C(O)—0- [I] 0 " In preferred embodiments of the invention, n is 0 to 1, and the phosphonoacid is phosphonoformic acid or phosphonoacetic acid. Lipid Derivatives of the Invention: Any lipid derivative of a phosphonoacid which possesses antiviral activity is within the scope of the invention. Compositions which will be most effective will have a lipid portion sufficient to be able to incorporate the material in a stable way into a liposomal bilayer, cell membrane, lipid bilayer, or other macromolecular array. Lipid groups attached to the phosphonoacids can be, for example, glycolipids, sphingolipids, phospholipids, glycerolipids, or fatty acids. The phosphonoacid can be conveniently linked to an available hydroxyl group of a lipid either through a carboxyester link or through a phosphoester link. Some preferred lipid derivatives of phosphonoacids are members of the following general classes: Antiviral diacylqlvcerol phosphonoacids One species of this class of antiviral lipid compounds has the structure: H j C-O-R, wherein n is 0 or 1; an aliphatic acid, either formic or acetic, is joined to P through a phosphonate bond to form the phosphonoacid; and R 1 and R 2 are aliphatic groups, defined below. Another species of this class of compounds is l, 2,- diacylglyceroloxycarbonyl phosphonoacids, having the structure: H 2 C 0 C-(CH 2 ) n —P—O [lib] O " wherein n = 0 or l, and R 1 and R 2 are C 2 . 24 aliphatic groups having 0 to 6 double bonds, and are linked to the C and C2 of a glycerol group through an ester linkage. 1-O-alkyl glycerol derivatives of phosphonoacids: One species of this class are those lipid derivatives havin the general structure: wherein n is 0 or 1; an aliphatic acid, either formic o acetic, is joined to P through a phosphonate bond; and R 1 i an aliphatic group defined below. Another species of this class of compounds has the structur [Illb wherein a phosphonoacid, either phosphonoformic, (n=0) , o phosphonoacetic (n=l) , is linked to the glycerol moiety b means of a carboxyester bond. Diacylglycerol phosphate phosphonoacids This class of antiviral phosphonoacid lipids has th general structure: wherein n is 0 or 1; an aliphatic acid group, either format or acetate, is joined to P through a phosphonate bond; and R and R 2 are aliphatic groups, defined below. Cera ide antiviral phosphonoacids: Antiviral phosphonoacids can also be generated in cell after liposomal delivery of ceramide derivatives thereo having the general structure shown below: 0 II CER—0 P—(CH 2 ) n —C(0) 0 " [V 0 " where n is 0 or 1 and CER is an N-acylsphingosine having th structure: CH=CH-(CH 2 ) 12 -CH 3 CHOH RC(0)NH-CH H 2 C- wherein R is an aliphatic group as defined below, or a equivalent lipid-substituted derivative of sphingosine. Thi class of compounds is useful in liposomal formulation in th therapy of AIDS and other viral diseases because it can b acted upon by sphingomyelinase or phosphodiesterases in cell giving rise to the antiviral phosphonoacid. In addition t the compound shown above, ceramide diphosphate phosphonoacid can also be synthesized, which may be degraded by cellula pyrophosphatases to give a phosphonoacid and ceramid phosphate. Lipjd-Rich Derivatives of Antiviral Phosphonoacids One approach to achieving even greater stability of lipi derivatives of phosphonoacids within liposomes is b increasing lipid-lipid interaction between the lipid phosphonoacid structure and the lipid bilayer. Accordingly in preferred embodiments, lipid derivatives of phosphonoacid having up to four lipophilic groups may be synthesized. Specific compositions are provided having the formula: < L ) m - (V) n - - T [VI] o wherein W is T is (CH 2 ) -C(0)-0-, wherein p = 0 or 1; V is phosphate; n = 0 to 2; L is a lipid moiety; m = 1 to 3; and wherein each L is linked directly to V in a phosphoester linkage and T is linked to W through a phosphonate bond. In another embodiment, compositions are provided having wherein W is V is phosphate; n = 0 or 1; T is (CH 2 ) -C(0)-0; p is 0 or 1; L is a lipid moiety; L, is (CH 2 -CHOH-CH 2 ). and wherein L and L 1 are each linked to V through a phosphoester bond and T is joined to W through a phosphonate bond. One class of these comprises diphosphatidylglycerol derivatives, having the general structure: 0 -P (CH 2 ) n C (0) 0 " 0 OH 0 H H H | H | H II H H H HC — C — C — 0 P — 0 C — C — C — 0 P O C — C — CH [ VI 11 ] | | H | H H H | H | | R., R 2 O " O " R 3 R 4 wherein n is 0 or 1, and R are two, three or four aliphatic groups which are independently R as defined below, said groups being in acyl ester, ether, or vinyl ether linkages. In this class, phosphonoacids are attached to one or both phosphates by a diphosphate bond. There may be one or two phosphonoacids attached to each molecule. Another class of phosphonoacid derivatives having increased lipid components comprises bis(diacylglycero)phosphatephosphonoacids, havingthe general structure: where n is 0 or 1, and R 1 - R 4 are as defined previously. This compound will be metabolized to a phosphonoacid in the cells by endogenous pyrophosphatases or other esterases. These two types of compounds may provide superior metabolic and physical properties. Lipid Structures Substituent R groups R 1 and R 2 , as well as R 3 _ A for the bis(diacylglycero) species) may be the same or different, and are C 2 to C 24 aliphatic groups, having from 0 to 6 sites of unsaturation, and preferably having the structure CH 3 -(CH 2 ) a -(CH=CH-CH 2 ) b ~(CH 2 ) C -Y wherein the sum of a and c is from 0 to 22; and b is 0 to 6; and wherein Y is C(0)0 " ' C-0 " ' C=C-0 " - C(0)S-, C-S-, C=C-S-; forming acyl ester, ether or vinyl ether bonds, respectively, between the aliphatic groups and the glycerol moiety. These aliphatic groups in acyl ester linkage therefore comprise naturally occurring saturated fatty acids, such as lauric, myristic, palmitic, stearic, arachidic and lignoceric, and the naturally occurring unsaturated fatty acids palmitoleic, oleic, linoleic, linolenic and arachidonic. Preferred embodiments comprise a onoester or diester, or a 1- ether, 2-acyl ester phosphatidyl derivative. In othe embodiments, the aliphatic groups can be branched chains o the same carbon number, and comprise primary or secondar alkanol or alkoxy groups, cyclopropane groups, and interna ether linkages. The glycero-phospho bonds may be racemic or sn-1 or sn-3 ester bonds; alternatively, the phosphate group may be joine at the 2-position of the glycerol moiety. There may be 1 o 2, (as well as 3, or 4 for the bis(diacylglycero) species) acyl ester groups, or alkyl ether or vinyl ether groups, a required. In any of the above embodiments, L is independentl selected from the group consisting of R; wherein R, R 1 and R 2 are independently C 2 to C 24 aliphati groups in ester, ether, or vinyl ether linkage. In any of th specific compositions described, R, R 1 and R 2 independentl have from 0 to 6 sites of unsaturation, and have th structure: CH 3 -(CH 2 ) a -(CH=CH-CH 2 ) fa -(CH 2 ) C ~Y wherein the sum of a and c is from 0 to 22; and b is 0 to 6; and wherein Y is C(0)0-, C-0-, C=C-0-, C(0)S, C-S, or C=C-S. Synthesis of Lipid Derivatives of Phosphonoacids This class of lipid derivatives of phosphonoacids wherei the lipid is joined through a phosphate ester link with th phosphate of the phosphonoacid, for example, the compounds o formula Ha, Ilia, IV, V, VI, VII, VIII, and IX, can b prepared as described in Examples 1, 2, and 5. Diacylglycerophosphonoformates of formula Ila, for example, can be synthesized from the reaction of a diacylglycerol an phosphonoformic acid using triisopropylbenzenesulfony chloride in pyridine as described for the preparation of 1,2- dimyristoyl-glycero-3-phosphophonoformate in Example 1, or l,2-dipalmitoyl-glycero-3-phosphophonoformate in Example 2. l-O-alkyl-s/ι-glycero-3-phosphonoacids, of the general formula Ilia, or 2-0-alkyl-5«-glycero-3-phosphonoacids, can be prepared according to Example 5, in a similar procedure. Lipid derivatives of phosphonoacids wherein the phosphonoacid is linked to the lipid group through a carboxyester bond with the carbonyl group of the phosphonoacid, of the formulas lib and Illb, can be prepared according to the method set forth in Examples 3 and 4. The unesterified glycerol hydroxyl groups having protective groups, such as benzyl. Other analogs, comprising l-acyl-2-0-alkylglycerols, l-o- alkyl-2-acylglycerols, dialkylglycerols, 1-acylglycerols, 2- acylglycerols, 2-O-alkylglycerols can be synthesized by the same methods, using similar protective groups as necessary. The diacylglycerol phosphate phosphonoacid class of compounds of formula IV may also be synthesized by preparing the morpholidate derivative of the phosphonoacid and coupling to phosphatidic acid in dry pyridine as described for cytidine diphosphate diglyceride by Agranoff and Suo i (23) . Alternatively, the morpholidate of phosphatidic acid may likewise be coupled to the phosphonoacid directly. Ceramide phosphonoacids of formula V may be prepared in a method similar to the method for preparing antiviral phosphonoacid diphosphate diglycerides, with appropriate changes to the starting materials. The bis(diacylglycero)phosphate phosphonoacid compounds of formula IX may be synthesized by joining a phosphonomorpholidate to the phosphoester residue of bis(diacylglycero)phosphate by the condensation methods presented in the Examples. Other suitable lipid derivatives of phosphonoacids may be synthesized using the same methods and using appropriate novel lipids as starting materials. It is desirable, for example, to synthesize phospholipid derivatives of antiviral and antiretroviral phosphonoacids which will give rise to potent antiviral agents upon alternate paths of metabolism by the target cells which take up the lipid formulation. Liposome Preparation After synthesis and purification, the lipid derivative of the phosphonoacid is incorporated into liposomes, or other suitable carrier. The incorporation can be carried out according to well known liposome preparation procedures, such as sonication and extrusion. Suitable conventional methods of liposome preparation include, but are not limited to, those disclosed by Bangham, et al. (9) Olson, et al. (24), Szoka and Papahadjapoulos (25), Mayhew, et al. (26), Kim, et al. (27), and Mayer, et al. (28). The liposomes may be made from the lipid derivatives of phosphonoacids alone or in combination with any of the conventional synthetic or natural phospholipid liposome materials including phospholipids from natural sources such as e< ?9 / plant or animal sources such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingomyelin, phosphatidylserine, or phosphatidylinositol. Synthetic phospholipids that may also be used, include, but are not limited to: dimyristoylphosphatidylcholine , dioleoylphosphatidyl-choline, dipalmitoylphosphatidylcholine and distearoylphosphatidycholine, and the corresponding syntheticphosphatidylethanolaminesandphosphatidylglycerols. Other additives such as cholesterol or other sterols, cholesterol he isuccinate, glycolipids, cerebrosides, fatty acids, gangliosides, sphingolipids, l,2-bis(oleoyloxy)-3- (tri ethyl ammonio)propane (DOTAP) , N-[l-(2,3-dioleoyl) propyl]-N,N,N-trimethylammonium chloride (DOTMA) , DL-2,3- distearoyloxypropyl(dimethyl)- -hydroxyethylammonium (29), or psychosine can also be added, as is conventionally known. The relative amounts of phospholipid and additives used in the liposomes may be varied if desired. The preferred ranges are from about 80 to 95 mole percent phospholipid (including the lipid phosphonoacid) and 5 to 20 mole percent psychosine or other additive. Cholesterol, cholesterol hemisuccinate, fatty acids or DOTAP may be used in amounts ranging from 0 to 50 mole percent. The amounts of antiviral phosphonoacids and nucleoside analogue incorporated into the lipid layer of liposomes can be varied with the concentration of their lipids ranging from about 0.01 to about 100 mole percent. Using conventional methods, approximately 20 to 30% of the phosphonoacid present in solution can be entrapped in liposomes; thus, approximately 70 to 80% of the active compound is wasted. In contrast, where the lipid phosphonoacid is incorporated into liposomes, virtually all of the antiviral compound is incorporated into the liposome, and essentially none of the active compound is wasted. The liposomes with the above formulations may be made still more specific for their intended targets with the incorporation of monoclonal antibodies or other ligands specific for a target. For example, monoclonal antibodies to the CD4 (T4) receptor may be incorporated into the liposome by linkage to phosphatidylethanolamine (PE) incorporated into the liposome by the method of Leserman, et al. (30). Therapy of Viral Diseases The lipid derivatives of the present invention or liposomes comprising these antiviral agents and containing 0.1 to 100% of a lipid derivative such as batyl alcohol phosphonoformic acid or phosphatidylphosphonoformic acid (phosphatidyl-PFA) or phosphatidylphosphonoacetate (phosphatidyl-PAA) or antiviral analogues of this class of compounds may be administered by parenteral routes to persons infected with HIV or cytomegalovirus (CMV) . These liposomes can be given to AIDS or CMV patients by parenteral administration, enhancing delivery of the compound to macrophages and monocytes, an important reservoir of viral infections. This will allow for the efficacious use of lower doses of the modified phosphonoacids, reducing toxicity of the compound. These antiviral agents may be used alone or in combination with other antiviral nucleosides of this general class as given conventionally. In addition, it is important to note that liponucleotides of AZT, ddC, ddA, ddl, d4T and ddT as disclosed in a copending application (22) may also be incorporated into the phosphatidyl-PFA liposomes singly or in combination to produce pPFA/dideoxynucleoside combination therapies. Alternatively, phosphate esters of the dideoxynucleosides may be encapsulated in the interior of the pPFA liposome as previously described (16) to produce a combination therapy for HIV infections. These combination therapies with AZT and phosphatidyl-PFA may be especially important in order to treat more effectively the AZT-resistant (or nucleoside-resistant) strains which have been seen to develop during single drug therapy (31) . The use of combination therapy as outlined may greatly reduce the tendency for drug resistant HIV mutant strains to appear and would therefore increase the likelihood of stopping the progression of HIV infection. The same argument would hold equally well in treating cytomegalovirns or herpes virus infections with regard to the likelihood of developing resistant strains. The liposome-incorporated lipid-phosphonoacid conjugate is administered to patients by any of the known procedures utilized for administering liposomes. The liposomes can be administered intravenously, intraperitoneally, intramuscularly, intravitreally or subcutaneously as a buffered aqueous solution. Any pharmaceutically acceptable aqueous buffer or other vehicle may be utilized so long as it does not destroy the liposome structure or the activity of the lipid phosphonoacid analogue. One suitable aqueous buffer is 150 mM NaCl containing 5 mM sodium phosphate with a pH of about 7.4 or other physiological buffered salt solutions. The dosage for a mammal, including a human, may vary depending upon the extent and severity of the infection and the activity of the administered compound. Dosage levels for the phosphonoacids are well established (2,6,16). Dosage levels of liposomal lipid analogs of phosphonoacids will be about the same as for the phosphonoacid itself, but in general, should be such that about 0.1 mg/kilogram t 1000 mg/kilogram is administered to the patient on a dail basis and more preferably from about 1 mg/kilogram to abou 100 mg/kilogram. The present invention utilizes the antivira phosphonoformate derivatives noted above incorporated i liposomes in order to direct these compounds to macrophages monocytes and any other cells which take up the liposoma composition. Ligands may also be incorporated to furthe focus the specificity of the liposomes. The derivative described have several unique and novel advantages over t liposomal water soluble phosphonoformates. First, they can b formulated in liposomes to much higher ratios of drug to lipi because they are incorporated into the wall of the liposo instead of being located in the aqueous core compartmen Secondly, the liposomes containing the lipophili phosphonoformate derivatives noted above do not leak duri storage, providing improved product stability. Furthermore these compositions may be lyophilized, stored dry at roo temperature, and reconstituted for use, providing improve shelf life. They also permit efficient incorporation o antiviral compounds into liposomal formulations withou significant waste of active compound. A further advantage i that the compositions used in vivo treatment cause a large percentage of the administered antiviral lipid-phosphonoaci conjugate to reach the intended target. At the same time th use of the compositions reduces the amount being taken up cells in general, thereby decreasing the toxic side effects the nucleosides. The toxic side effects of t phosphonoformates may be further reduced by targeting t liposomes in which they are contained to actual or potenti sites of infection by incorporating ligands into t liposomes. Lipid derivatives of antiviral agents have a prolong antiviral effect as compared to the lipid-free agent therefore they provide therapeutic advantages as medicamen even when not incorporated into liposomes. Non-1iposom lipid derivatives of antiviral phosphonoacids may be applie to the skin or mucosa or into the interior of the body, fo example orally, intratracheally or otherwise by the pulmonar route, enterally, rectally, nasally, vaginally, lingually, intravenously, intra-arterially, intramuscularly, intraperitoneally, intradermally, or subcutaneously. Th present pharmaceutical preparations can contain the activ agent alone, or can contain further pharmaceutically valuabl substances. They can further comprise a pharmaceuticall acceptable carrier. Pharmaceutical preparations containing lipid derivative of antiviral phosphonoacids are produced by conventiona dissolving and lyophilizing processes to contain fro approximately 0.1% to 100%, preferably from approximately 1 to 50% of the active ingredient. They can be prepared a ointments, salves, tablets, capsules, powders or sprays, together with effective excipientε, vehicles, diluents, fragrances or flavor to make palatable or pleasing to use. Formulations for oral ingestion are in the form o tablets, capsules, pills, ampoules of powdered active agent, or oily or aqueous suspensions or solutions. Tablets or othe non-liquid oral compositions may contain acceptabl excipients, known to the art for the manufacture o pharmaceutical compositions, comprising diluents, such a lactose or calcium carbonate; binding agents such as gelati or starch; and one or more agents selected from the grou consisting of sweetening agents, flavoring agents, coloring o preserving agents to provide a palatable preparation Moreover, such oral preparations may be coated by know techniques to further delay disintegration and absorption i the intestinal tract. Aqueous suspensions may contain the active ingredient i admixture with pharmacologically acceptable excipients comprising suspending agents, such as methyl cellulose; an wetting agents, such as lecithin or long-chain fatty alcohols The said aqueous suspensions may also contain preservatives coloring agents, flavoring agents and sweetening agents i accordance with industry standards. Preparations for topical and local application compris aerosol sprays, lotions, gels and ointments i pharmaceutically appropriate vehicles which may comprise lowe aliphatic alcohols, polyglycols such as glycerol, polyethylen glycol, esters of fatty acids, oils and fats, and silicones The preparations may further comprise antioxidants, such a ascorbic acid or tocopherol, and preservatives, such as p hydroxybenzoic acid esters. Parenteral preparations comprise particularly sterile o sterilized products. Injectable compositions may be provide containing the active compound and any of the well know injectable carriers. These may contain salts for regulatin the osmotic pressure. The therapeutically effective amount of the lipi derivatives is determined by reference to the recommende dosages of the active antiviral phosphonoacid, bearing in min that, in selecting the appropriate dosage in any specifi case, consideration must be given to the patient's weight general health, metabolism, age and other factors whic influence response to the drug. The parenteral dosage will b appropriately an order of magnitude lower than the oral dose A more complete understanding of the invention can b obtained by referring to the following illustrative examples which are not intended, however, to unduly limit th invention. EXAMPLE 1 Synthesis of l,2-dimyristoyl-glycero-3-phosphonoformate. To a three necked reaction flask was added 0.620 grams o phosphonoformate (PFA; Sigma Chemical Co., St. Louis, MO dissolved in 4 ml of dry pyridine (Aldrich, Milwaukee, WI) an 1.28 grams of triisopropylbenzenesulfonyl chloride (Aldrich) 40 ml of 1,2 dimyristoylglycerol (10 mg/ml; Avanti Pola Lipids, Pelham, Alabama) was evaporated and redissolved in 1 ml of freshly distilled dry chloroform and added dropwise t the reaction mixture over a period of 30 minutes. Th reaction mixture was stirred overnight at room temperature After 24 hours, the reaction was stopped by the addition of 5 ml of cold 0.1N HCL. The organic phase was separated, drie over P 2 0 5 and evaporated under vacuum. The product wa crystallized from chloroform and acetone at 20°C. Thin-laye chromatography of the crystallized product, developed in basic system of chloroform, methanol, ammonia and wate (70/30/1/1 by volume) gave three phosphorus-containin products. Further purification of the required compound wa obtained by column chromatography. The product, in 50 ml o chloroform, was loaded onto 40 gms of silica gel G, 70-20 mesh, in a glass column measuring 1 x 14 inches an sequentially eluted with 200 ml of chloroform, 200 ml o chloroform/methanol (1:5), 200 ml of chloroform/methano (1:10), and finally 300 ml of chloroform/methanol (1:1) . Pur l,2-dimyristoyl-3-phosphonoformate was recovered in th chloroform/methanol (1:1) fraction. The purity of the compound was checked using two solven systems, chloroform, methanol, ammonia, and water (70/30/1/1) and chloroform, methanol, ammonia, and water (65/35/4) . Th pure compound has an Rf value of 0.54 in the former basi system. EXAMPLE 2 Synthesis of l,2-dipalmitoyl-glycero-3-phosphonoformate. 1 gram of phosphonoformic acid (PFA) , trisodium sal (Fluka, Ronkonkoma, NY) was dissolved in 50 ml of distille water and passed through a 1.4 X 10 cm column of Dowex 50-X (H+) , 200-400 mesh. The PFA-H+ was eluted with water lyophilized overnight, and dried for 48 hours over P 2 0 5 in vacuum oven at room temperature. To a 50 ml round botto flask containing 15 ml of dry pyridine (Aldrich, Milwaukee WI) was added 120 mg 1,2-dipalmitoylglycerol (Sigma, St Louis, MO) and 230 mg of PFA-H+ and 527 mg o triisopropylbenzenesulfonyl chloride (TPS, Aldrich Milwaukee WI) . The vessel was sealed with a rubber serum stopper an flushed with argon. The reaction was stirred overnight unde argon and after 24 hours, 15 ml of chloroform/methanol (1:2 was added and the product was precipitated by the addition o 15 ml of acetone at -20°C. The precipitate was collected an recrystallized from chloroform/acetone to obtain 1,2 dipalmitoylglycero-sn-3-phosphonoformate in yield of abou 70%. The product was taken up in a small volume o chloroform/methanol (1:2) and a small aliquot was applied t silica gel G plates (Uniplate, Analtech) and developed wit either chloroform/methanol/conc. ammonia/water (70/30/1/1 b volume) or chloroform/methanol/water (65/35/6) . The produc was present as a phosphorus-positive spot with Rf values o 0.69 or 0.80, respectively. The purity of the compound wa estimated to be 0% by visual inspection after charring wit concentrated sulfuric acid. Further purification was achieved by subjecting a portio of the crude product to preparative thin layer chromatograph using 20 x 20 cm plates of silica gel G (silica thickness 0. mm, Analtech) developed with chloroform/methanol/conc ammonia/water (70/30/1) . The compound was located by sprayin reference guide spots on the outside edges of the plate wit phosphorus spray, taking care to cover the surface of th remainder with a clean glass plate. The spot representing th product was scraped and extracted wit chloroforom/methanol/water (1/2/0.8). The chloroform phas was separated by the addition of chloroform/water (1/1) s that the final ratio of solvents is 1/1/0.9 as described b Bligh and Dyer (32) . The chloroform phase was separated an the solvent was evaporated in vacuo and the product was drie by lyophilization from cyclohexane. The purified product gav single spots upon thin layer chromatography in the two system noted above. EXAMPLE 3 Synthesis of l-0-octadecyl,2-0-benzyl-sn-glycero-3- oxycarbonylphosphonate (C-batylPFA) . A solution of 1-0-octadecyl, 2-O-benzyl-sn-glycerol (OBG) (Bachem Bioscience, Philadelphia, Pennsylvania) (0.68g) in tetrahydrofuran was added over a period of 1.5h to a solution of phosgene (1.5eg) in triethyla ine (5ml) while maintaining the temperature at 0°C. After the addition was completed the reaction mixture was stirred at 0°C for 4h. At the end of which time trimethylphosphite (4equiv) was added at 0°C and the reaction mixture was gradually warmed to room temperature overnight. The intermediate bis(methoxy)phosphonate was demethylated and the crude product was purified by chromatography to afford the title compound in 40% yield. EXAMPLE 4 Synthesis of 1-θ-octadecyl , 2-0-benzyl-sn-glycero-3- oxycarbonylphosphoacetic acid (C-batylPAA) . A solution of OBG alcohol (0.68g) in tetrahydrofuran was added over a period of lh to a cooled (-50° to -55°C) solution of (dichlorophosphinyl) acetyl chloride (0.6g) and the mixture was stirred for lh at -30°C and gradually warmed to 0°C. The solvent was evaporated and the residue treated with methanol (5ml) and the mixture allowed to stir at room temperature for 4h, At the end of which time the solvent was removed under reduced pressure and the solid was filtered and the solid was washed with ice cold methanol to afford the desired product in 50% yield. EXAMPLE 5 Synthesis of Batyl-Phosphonoformate A quantity of 0.9 grams of racemic batyl alcohol (1-0- octadecyl-2, 3-glycerol, Sigma Chemical, St. Louis, M0), 2.6 grams of triisopropylbenzenesulfonyl chloride (TPS, Aldrich, Milwaukee, WI) and 0.16 grams of phosphonoformate, acid form, were reacted in 15 ml of dry pyridine at room temperature under nitrogen. The reaction was monitored at half hour intervals by thin layer chromatography as in Example 3 and was judged to be complete at about 24 hours. The reaction was stopped by the addition of 10 ml of chloroform/methanol/ water (1/2/0.8 by volume). The organic (lower) phase was separated by further addition of 2 ml of chloroform and 2 ml of water. The organic phase was removed and evaporated in vacuo and the product was obtained as a white powder. The crude product was dissolved in a small volume of chloroform/methanol (1/1 by volume) and subjected to preparative thin layer chromatography using 0.5 mm layer of silica gel G plates (Analtech, Newark, DE) developed with chloroform/methanol/concentrated ammonia/water (70/30/1/1) . Two PFA-containing spots were visualized, scraped and extracted with chloroform/methanol/water as described earlier in Example 3. The two compounds are referred to as batyl-PFA, top and bottom, respectively. EXAMPLE 6 Liposome Formation with Phosphatidyl-PFA To a sterile 2.0 ml sonication vessel were added (in chloroform solution) 7.5 μmoles of dioleoylphosphatidyl choline, 4.5 μmoles of cholesterol, and 3 μmoles of pPFA. The solvent was removed in vacuo, forming a thin film of the lipid mixture. The lipid film was hydrated with 0.3 ml of sterile 10 mM sodium acetate buffer (pH 5.0) containing isotonic dextrose. The mixture was vortexed intermittently for 10 minutes followed by sonication for 90 to 120 min using the cup horn of a Heat Systems Ultrasonics sonicator (Model 43IB) at output control setting #9, which treatment resulted in clarification of the sample. This sample was diluted with sterile RPMI tissue culture medium and used in HIV experiments at the contractions indicated. EXAMPLE 7 Demonstration of Anti-HIV Activity in HT4-6C Cells HT4-6C cells were grown in RPMI 1640 medium containing 100 U/ml penicillin G, 100 ug/ml streptomycin, 2 mM gluta ine and 10% fetal bovine serum (Hyclone Laboratories, Logan, UT) . Cells were infected with HIV-1 (LAV-1 strain, L. Montagnier, Paris, France) at a multiplicity of infection sufficient to give 100-300 plaques per well in the no drug controls. Virus was allowed to adsorb at 37°C for one hour. Liposomes containing the two batyl-PFAs were prepared as described in Example 3. The sonicated preparation was diluted with sterile RPMI buffer and added to the tissue culture wells at the indicated concentrations. After a 3-day incubation at 37°C, the cell monolayers were fixed with 10% formaldehyde and stained with 0.25% crystal violet to visualize plaques. The straining procedure showed individual dense foci of multinucleated giant cells which were counted and used to assess antiretroviral drug activity. The results of the experiment are shown in Figure 1. Both preparations of batyl-PFA (top spot and bottom spot) were active in this experiment. The amount of drug require to produce 50% inhibition (I.C 50 ) can be estimated from the figure as follows: PFA 200 uM; batyl-PFA (top) 110 uM and batyl-PFA (bottom) 180 uM. EXAMPLE 8 Inhibition of human cytomegalovirus-specific DNA by phosphonoformate and phosphonoacetate lipid prodrugs. Assay Method: MCR-5 (human lung fibroblast) cells (about 5 x 10 4 cells per well of a 24 well plate) are plated in DME media with 10% fetal bovine serum one to two days before dru addition. The drugs in dimethylsulfoxide (DMSO) were added t the medium in a final DMSO concentration of 1%. In some cases where the compound was not readily soluble in water or DMSO, liposomes were prepared by sonication containing 10 mole% drug/50 mole% dioleoylphosphatidylcholine/30 mole% cholesterol and 10 mole% dioleoylphosphatidylglycerol. Control liposome were also prepared without the added drug and incubated a matched lipid concentrations as controls. This media is the aspirated and changed to the drug dilutions made in 2% FB media and incubated for 24 hours. The drug-containing medi is transferred to sterile tubes and a 1:50 dilution (abou 20,000 PFUs) of the AD-169 human CMV virus pool is added t the wells at 0.2 ml per well and incubated at 37 C for 60 min. The inoculum is made in DME plus 2% FBS media. After th incubation, the inoculum is aspirated from the wells and th drug dilutions are added back. Cells are incubated for fiv to six days when they should show a 50-100% cytopathic effect. At the end of the 5 to 6 day incubation period, th quantification of the CMV DNA present is done by nucleic aci hybridization using a CMV antiviral susceptibility test ki from Diagnostic Hybrids (Athens, OH) . The media from eac well is aspirated completely and two drops of lysis solutio (DNA wicking agent) is added to each well. After about fiv seconds the Hybriwix filter pairs are placed in the wells i numerical order and the solution is absorb onto the filter for about 30 minutes or until the wells are dry. Usin forceps, the Hybriwix are removed and placed on a paper towel. Each pair of Hybriwix are cut vertically from the share bridge of the filter. The Hybriwix are transferred to vial containing I 125 labelled CMV Probe Hybridization Agent ( maximum of 24 Hybriwix per vial) along with three negativ control Hybriwix and two positive control Hybriwix per vial. The vials are placed in a 60°C gently shaking water bat for a minimum of two hours to overnight. After th hybridization, the solution is aspirated from the vials an collected into a radioactive waste container. 4ml o distilled water is added to each vial to rinse, capped, swirled and then aspirated. 6ml of Wash Reagent is added t the Wash Container and the Hybriwix are transferred to thi and gently swirled. 114 ml of distilled water that has bee pre-heated to 73 C is added to the Wash Container and this i placed in a 73° C water bath for 30 minutes. The was solution is then removed to a radioactive waste container an the Hybriwix are placed in numerical order on a paper towe and then are transferred to gamma counting tubes and counte for two minutes. Results: After subtraction of the blank value, result are expressed as the percentage of the no drug control. Th concentration dependence of the CMV-DNA production was plotte and the amount of the respective drugs required to reduce th control level by 50%(IC 50 ) was determined and the results ar shown in the following table. EXAMPLE 9 Effect of Phosphonoformate, Phosphonoacetate and Various Lipi Analogs on Production of hcmv-specific DNA by mrc-5 Human Lun Fibroblasts in vitro. COMPOUND ic, 50 PFA DMG-PFA BATYL, BENZYL-PFA PAA DMG-PAA DMP-PAA LIPOSOME CONTROL 125 * Liposome formulation. All other data obtained wit compounds dissolved directly in 1% DMSO in Dulbecco' modified-Eagle's medium. Abbreviations: PFA, phosphonoformic acid; PAA, phosphonoacetic acid; DMG-PFA, l,2-dimyristoyl-5n-glycero-3-phosphonoformic acid Batyl, benzyl-PFA, 1-0-octadecyl, 2-benzyl-sn-glycero-3 phosphonoformic acid; DMG-PAA, 1,2-dimyristoyl-sn-glycero-3-phosphonoacetic acid DMP-PAA, l,2-dimyristoyl-5«-glycero-3-phospho-phosphonoaceti acid. As demonstrated in the preceding table, all lipid analogs of PFA and PAA exhibited activity in reducing the production of human CMV-specific DNA without apparent toxicity as determined by visual inspection of the cell monolayers. Liposome controls reduced CMV-specific DNA production at ver high lipid doses but the activity of liposomal batyl, benzyl- PFA was 74 times more active than matched liposome controls without the lipid drug. It should be apparent from the foregoing that othe phosphonoacid compounds and lipid derivatives thereof can b substituted in Examples 3 and 5 to obtain similar results. Other antiviral agents, such as, for example, nucleosid analogue phosphates, may also be contained in the aqueou compartments of the liposome (7) . The molar percentage of th lipid antiviral agents, such as, for example, nucleosid analogue phosphates, may also be contained in the aqueou compartments of the liposome (7) . The molar percentage of th lipid antiviral agent may vary from 0.1 to 100% of the total lipid mixture. Furthermore, mixtures of antiviral nucleosid lipids may be used in constructing the liposomes for therap of viral diseases (6) . It should be further emphasized tha the present invention is not limited to the use of an particular antiviral phosphonoacid; rather, the beneficia results of the present invention flow from the synthesis o the lipid derivatives of these materials and the use o liposomes for formulations for the treatment of vira diseases. Thus, regardless of whether a specific antivira phosphonoacid is presently known, or whether it becomes know in the future, the methods of forming the presently contemplated lipid derivatives therefrom are based o established chemical techniques, as will be apparent to thos of skill in the art, and their incorporation into liposomes i broadly enabled by the preceding disclosure. It should b emphasized again that the present syntheses are broadl applicable to formation of compounds from essentially a phosphonoacids for use in the practice of the presen invention. Accordingly, the invention may be embodied in othe specific forms without departing from it spirit or essentia characteristics. The described embodiments are to b considered in all respects only as illustrative and no restrictive, and the scope of the invention is, therefore indicated by the appended claims rather than by the foregoin description. All modifications which come within the meanin and range of the lawful equivalency of the claims are to b embraced with their scope. REFERENCES 1. Helgestrand, E., et al. (1978) Science (Washington, D.C.) 201:819. 2. U.S. Patent No. 4,771,041 to Eriksson, et al. 3. Fischl, M.S., Richman, D.D., Grieco, M.H. , et al. (1987) New Eng. J. Med. , 317: 185-191. 4. Richman, D.D., Fischl, M.A. , Grieco, M.H., et al. (1987) New Eng. J. Med., 317:192-197. 5. Richman, D.D., Kornbluth, R.S. and Carson, D.A. (1987) J. Exp. Med., 166: 11444-1149. 6. Lambert, R. , et al. (1989) J. Med. Chem. 32:367- 374. 7. Matsushita, T. , Ryu, E.K., Hong, C.I. and MacCoss, M. (1981) Cancer Res., 41: 2707-2713. 8. Ho, D.W.H. and Neil, B.L. (1977) Cancer Res., 37:1640-1643. 9. Bangham, A.D., Standish, M.M. and Watkins, J.c. (1965) J. Mol. Biol. 23:238-252. 10. Black, CD.V., Watson, G.J. and Ward, R.J. (1977) Trans. Roy. Soc. Trop. Med. Hyg. , 71:550-552. 11. Alving, C.R. , Steck, E.a., Chapman, W.L. , Waits, V.B., Hendricks, L.D. Swartz, G.M. and Hanson, W.L. (1978) Proc. Natl. Acad. Sci. USA 75:2959-2963. 12. Lopez-Berestein, G. (1986) Ann. Int. Med., 103: 694-699. 13. Herman, E.H. , Rahman, A., Ferrans, V.J. Vick, J.A. and Shein, P.S. (1983) Cancer Res., 43: 5427-5432. 14. Ostro, M. (1987) Sci. Am. 256:103-111. 15. Oberg, B. , Pharmacol. Ther. 19:387-415 (1983). 16. Szoka, F. and Chu, C-J. , Antimicrobial Agents and Chemotherapy: 32(6) 858-864 (1988) . 17. Salahuddin, S.Z., Rose, R.M. , Groopman, J.E. , Markham, P.D. and Gallo, R.C. (1985) Blood, 68:281- 284. 18. Koenig, S., Gendelman, H.E., Orenstein, J.M., Dalcanto, M.C. Pezeshkpur, G.H. , Yungbluth, M. Janotta, F. , et al. (1986) Science, 233:1089-1093. 19. Post, G., Kirsch, R. and Koestler, T. (1984) in Liposome Technology, Vol. Ill, G. Gregoriadis, Ed., CRC Press, Boca Raton, p. 1-28. 20. Scherphof, G. (1986) in Lipids and Biomembranes, Past, Present and Fuure, Op den Kamp, J., Roelofsen, B. and Wirtz, K.W.A. , Eds., Elsevier North Holland, Amsterdam, p. 113-136. 21. Hostetler, K.Y., U.S. Patent Application for LIPID DERIVATIVES OF ANTIVIRAL NUCLEOSIDES FOR LIPOSOMAL INCORPORATION ...Serial No. 373,088 (1989). 22. Hostetler, K.Y. and D.D. Richman, U.S. Patent Application Serial No. 099,755for METHOD OF PREPARING ANTIVIRAL DRUGS FOR LIPOSOME ENCAPSULATION ...(1987). 23. Agranoff, B.W. and W.D. Suomi, Biochemical Preparations 10:46-51 (1963). 24. Olson, F. , Hunt, CA. Szoka, F.C, Vail, W.J. and Papahadjopoulos, D. (1979) Biochim, Biophys. Acta, 557: 9-23. 25. Szoka, F. , and Papahadjopoulos, D. (1978) Proc. Nat. Acad. Sci. 75:4194-4198. 26. Mayhew, E., Lazo, R. , Vail, W.J., King, J., Green, A.M. (1984) 775:169-175. 27. Kim, S., Turker M. , Chi, E. , et al. , Biochim. Biophys. Act, 728:339:348. 28. Mayer, L.D., Hope, M.J. and Cullis, P.R. (1986) Biochim. Biophys. Acta, 858:161-168. 29. Rosenthal, A.F. and R.P. Geyer (1960) J. Biol. Chem. 235(8) :2202-2206. 30. Leserman, L.D., Barbet, J. and Kourilsky, F. (1980) Nature 288:602-604. 31. Larder, B.A. , Darby, G. and Richman, D.D. (1989) Science 243:1731-1734. 32. Bligh, E.G. and W.J. Dyer Can. J. Biochem.. 37: 911 (1959) .

Claims: WHAT IS CLAIMED IS: 1. A compound having antiviral activity, comprising: a phosphonoacid linked to a lipid selected from the group consisting of phospholipids, glycerolipids, glycolipids, sphingolipids, or ceramides. 2. The compound of Claim 1, wherein said phosphonoacid is linked to an available hydroxyl group of said lipid through a phosphate ester bond. 3. The compound of Claim 1, wherein said phosphonoacid is linked to an available hydroxyl group of said lipid through a carboxyester bond. 4. A compound according to Claim 2, having the structure: wherein n = 0 or 1; and R 1 is a C 2 . 24 aliphatic group, having 0 to 6 double bonds, linked to a glycerol hydroxyl group through an ether or a vinyl ether linkage. 5. A compound according to Claim 2 having the structure H 2 C-0-R 1 wherein n = 0 or 1; and R 1 and R 2 are C 2 . 24 aliphatic groups, having 0 to 6 double bonds, linked to a glycerol hydroxyl group thorugh an acyl ester linkage. 6. A compound according to Claim 3, having the structure wherein n = 0 or 1, and R., is a C 2 . 24 aliphatic group having 0 to 6 double bonds, and is linked to a glycerol hydroxyl group through an ether or vinyl ether linkage. 7. A compound according to Claim 3 having the structure H^-O-R, wherein n = 0 or 1, and R 1 and R 2 are C 2.24 aliphatic groups having 0 to 6 double bonds, and R., and R 2 are each linked to a glycerol hydroxyl group through an acyl ester linkage. 8. The compound of Claim 1 wherein said lipid is selected from the group consisting of a monoacylglycerol, a monoalkylglycerol, a diacylglycerol, a dialkylglycerol, a 1-acyl, 2-O-alkyl-glycerol, and a 1-O-alkyl, 2-acyl-glycerol. 9. A compound according to Claim 4 that is a 1-0- alkyl-sn-glycero-3-phosphonoacid. 10. A compound according to Claim 9 that is a l-O-hexadecyl-.srt-glycero-3-phosphonoacid. 11. A compound according to Claim 9 that is a l-0-octadecyl-srt-glycero-3-phosphonoacid. 12. A compound according to Claim 5 that is a 1,2- diacyl-Λ/ι-glycero-3-phosphonoacid. 13. A compound according to Claim 12 that is a 1,2-dimyristoyl-sn-glycero-3-phosphonoacid. 14. A compound according to Claim 12 that is a 1,2-dipalmitoyl-srt-glycero-3-phosphonoacid. 15. A compound according to Claim 6 that is a 1-0- alkyl-.sn-glycero-3-oxycarbonylphosphonoacid. 16. A compound according to Claim 15 that is a l-O-hexadecyl-.m-glycero-3-oxycarbonylphosphonoacid.. 17. A compound according to Claim 15 that is a l-0-octadecyl-5«-glycero-3-oxycarbonylphosphonoacid. 18. A compound according to Claim 7 that is a 1,2- diacyl-srt-glycero-3-oxycarbonylphosphonoacid. 19. A compound according to Claim 18 that is a l,2-dimyristoyl-5/7-glycero-3-oxycarbonylphosphonoacid. 20. A compound according to Claim 18 that is a 1,2-dipalmitoyl-5«-glycero-3-oxycarbonylphosphonoacid. 21. The compound of Claim 1 wherein said lipid is a phospholipid. 22. A lipid-rich derivative of a phosphonoacid of the formula (L) m - (V) n - W - T wherein W is T is (CH 2 ) -C(0)-0-, wherein p = 0 or 1; V is phosphate; n = 0 to 2; L is a lipid moiety selected from the group consisting of phospholipids, sphingolipids, glycolipids, ceramides or glycerolipids; m = 1 to 3; and wherein each L is linked directly to a V group in a phosphoester linkage and T is linked to W through a phosphonate bond. 23. A liposome formed in part from any one of the compounds of Claims 1 to 7 and 22. 24. A method for treating a viral infection in a mammal, comprising administering an effective amount of a compound according to Claim 1 to said mammal. 25. The method of Claim 24, wherein said mammal is a human and said retrovirus is human immunodeficiency virus (HIV) . 26. The method of Claim 25, further comprising the step of administering an effective amount of 3'-azido-3'- deoxythymidine (AZT) to said mammal. 27. A method for treating HIV infection in a mammal, wherein said method includes avoiding or overcoming resistance to AZT or to other nucleoside analogues by administering said analogues in combination with the compounds of any one of Claims 1 to 7 and 22. 28. A method for preparing a suspension of liposomes for use in treating viral infections in a mammal, comprising the steps of: (a) providing an amphipathic antiviral agent comprising a phosphonoacetic acid or phosphonoformic acid linked to a lipid selected from the group consisting of phospholipids, glycerolipids, glycolipids, sphingolipids, ceramides or fatty acids; (b) combining said amphipathic antiviral agent and a pharmacologically acceptable aqueous solvent to form a mixture; and (c) forming liposomes from said lipophilic antiviral agent. AMENDED CLAIMS [received by the International Bureau on 31 August 1993 (31.08.93); original claims 23 and 27 amended; remaining claims unchanged (2 pages)] 1, 2-dimyristoyl-.v/7-glycero-3-phosphonoacid. 1 . A compound according to Claim 12 that is a 1 , 2-dipalmitoy l-s/ι-glycero-3 -phosphonoacid . 15. A compound according to Claim 6 that is a l-o- alkyl-.m-glycero-3-oxycarbonylphosphonoacid. 16. A compound according to Claim 15 that is a l-O-hexadecyl-.ϊrt-glycero-3-oxycarbonylphosphonoacid.. 17. A compound according to Claim 15 that is a l-O-octadecyl-^n-glycero-3-oxycarbonylphosphonoacid. 18. A compound according to Claim 7 that is a 1,2- diacyl-sn-glycero-3-oxycarbonylphosphonoacid. 19. A compound according to Claim 18 that is a 1 , 2-dimyristoyl-sn-glycero-3-oxycarbonylphosphonoacid . 20. A compound according to Claim 18 that is a l , 2-dipalmitoyl-.m-glycero-3-oxycarbonylphosphonoacid . 21. The compound of Claim 1 wherein said lipid is a phospholipid. 22. A lipid-rich derivative of a phosphonoacid of --the formula (L) m - (V) n - W - T 0 wherein W is 0 " T is (CH 2 ) -C(0)-0-, wherein p = 0 or 1; V is phosphate; n = 0 to 2; L is a lipid moiety selected from the group consisting of phospholipids, sphingolipids, glycolipids, ceramides or glycerolipids; = 1 to 3 ; and wherein each L is linked directly to a V group in a phosphoester linkage and T is linked to W through a phosphonate bond. 23. A liposome formed in part from any one of the compounds of Claims 1-7 or 22. 24. A method for treating a viral infection in a mammal, comprising administering an effective amount of a compound according to Claim 1 to said mammal. 25. The method of Claim 24, wherein said mammal is a human and said retrovirus is human immunodeficiency virus (HIV) . 26. The method of Claim 25, further comprising the step of administering an effective amount of 3'-azido-3'- deoxythymidine (AZT) to said mammal. 27. A method for treating HIV infection in a mammal, wherein said method includes avoiding or overcoming resistance to AZT or to other nucleoside analogues by administering said analogues in combination with the compounds of any one of Claims 1-7 or 22. 28. A method for preparing a suspension of liposomes for use in treating viral infections in a mammal, comprising the steps of: (a) providing an amphipathic antiviral agent comprising a phosphonoacetic acid or phosphonoformic acid linked to a lipid selected from the group consisting of phospholipids, glycerolipids, glycolipids, sphingolipids, ceramides or fatty acids; (b) combining said amphipathic antiviral agent and a pharmacologically acceptable aqueous solvent to form a mixture; and (c) forming liposomes from said lipophilic antiviral agent.

Inventors: HOSTETLER KARL Y, KUMAR RAJ, HOSTETLER, KARL, Y., KUMAR, RAJ
Applicants: VESTAR INC, VESTAR, INC.
Application year: 1992
Main classification: C07F9
IPC codes: A61K9, C07F9, C07H15, C07H19