Hexapeptide

Abstract: A hexapeptide represented by formula (II): A-B-Pro-C-D-E (II) where A is the L- or D- form of arginine or lysine whose N-terminal amino group is alkylated or acylated, B is the L- or D- form of arginine, lysine, or histidine, Pro is the L- or D- form of proline, C is the L- or D- form of tyrosine, tryptophan, or phenylalanine, D is the L- or D- form of valine, isoleucine, or leucine, and E is the L- or D- form of valine, isoleucine, or leucine, one of the hydrogen atoms of the amino group of which may be substituted with a C₁ to C₄ alkyl group, and the C-terminal carboxyl group of which may be substituted with -COOR*, -CH₂OR, or -CONHR, wherein R* is a C₁ to C₄ alkyl group, and R is a hydrogen atom or an C₁ to C₄ alkyl group. The hexapeptide and pharmaceutically acceptable salts thereof are useful as medical agents, for example as an anti-edematous agent, an anti-shock agent, an anti-thrombus agent, an anti-arteriosclerotic agent, an anti-allergic agent, a hypotensive agent, a wound healing agent, and an anti-inflammatory agent.

Description: The present invention relates to a novel hexapeptide and a pharmaceutically acceptable salt thereof. The hexapeptide has an increasing vascular permeability suppression action, an anti-edematous action, an anti-inflammatory action, a vascular endothelial disorder amelioration action, a hypotensive action, a protease inhibition action, an anti-desseminated intravascular coagulation syndrome (DIC) action, and a wound healing action. The hexapeptide can be useful as a medical product such as an anti-edematous agent, an anti-shock agent, an anti-thrombus agent, an anti-arteriosclerotic agent, an anti-allergic agent, a hypotensive agent, a wound healing agent, and an anti-inflammatory agent. In a conventional therapy for edematous, such as a cerebral edema, a therapeutical method is most widely applied which uses an osmotic diuretic agent to elevate the hydrostatic pressure of the blood and introduce water into the blood. In this method, a hypertonic solution of a diuretic agent such as glycerol or mannitol is quickly injected intravenously three or four times a day. Each injection time takes about 30 minutes to an hour. This method, however, requires complicated administration control. The doses of the osmotic diuretic agent must be changed according to the pathology and condition of the patients. Further, administration of the osmotic diuretic agent mentioned above must be carefully controlled, since the agent causes side effects such as disturbance of an electrolyte and dehydration. Antibiotics are mainly used in conventional therapy for septicemia. But, since the antibiotics cannot suppress the increasing vascular permeability, shocks often cannot be remedied satisfactorily in the therapy using the antibiotics. Adrenal cortical hormones are also used together with the above antibiotics for suppressing the increasing vascular permeability. However, a large dose of steroids such as adrenal cortical hormones causes side effects such as an immuno-suppression, an electrolytic abnormality, and a rebound phenomenon. As described above, a medicament has not yet been found which can suppress the increasing vascular permeability in the edematous and the septicemia, can cause few side effects, and which does not require the complicated administration for dosage. Accordingly, an object of the present invention is to provide a novel compound which has an increasing vascular permeability suppression action, and causes few side effects, when administered. Another object of the invention is to provide a pharmaceutical agent which does not require complicated administration control. EP-A-0333071 and Eur. J. Biochem. (1982), 124 , pgs 117-125 both describe the synthesis of various neurotensin analogues and the pharmacological uses of such compounds. The present invention provides a hexapeptide represented by formula (I): A-B-Pro-C-D-E where A is the L- or D- form of arginine or lysine, the N-terminal amino group of which is deaminated, B is the L- or D- form of arginine, lysine or histidine, Pro is the L- or D- form of proline, C is the L- or D- form of tyrosine, tryptophan, or phenylalanine, D is the L- or D- form of valine, isoleucine, or leucine, and E is the L- or D- form of valine, isoleucine, or leucine, one of the hydrogen atoms of the amino group of which may be replaced by a C 1 to C 4 alkyl group, and the C-terminal carboxyl group of which is replaced by -CH 2 OR, wherein R is a hydrogen atom or a C 1 to C 4 alkyl group; or a pharmaceutically acceptable salt of the hexapeptide. The present invention also provides a hexapeptide represented by formula (II): A-B-Pro-C-D-E where A is the L- or D- form of arginine or lysine, the N-terminal amino group of which is alkylated or acylated, B is the L- or D- form of arginine lysine or histidine, Pro is the L- or D- form of proline, C is the L- or D- form of tyrosine, tryptophan, or phenylalanine, D is the L- or D-form of valine, isoleucine, or leucine, and E is the L- or D- form of valine, isoleucine, or leucine, one of the hydrogen atoms of the amino group of which may be replaced by a C 1 to C 4 alkyl group, and the C-terminal carboxyl group of which is replaced by -CH 2 OR, wherein R is a hydrogen atom or a C 1 to C 4 alkyl group: or a pharmaceutically acceptable salt of the hexapeptide. The hexapeptide or its salt of the invention has, in addition to the vascular permeability acceleration suppression action noted above, an anti-edematous action, an anti-inflammatory action, a vascular endothelial disorder amelioration action, a hypotensive action, a protease inhibition action, anti-DIC action, and a wound healing action. Thus, the hexapeptide or its salt of the invention may be useful as an active ingredient for a pharmaceutical agent such as an anti-edematous agent, an anti-shock agent, an anti-thrombus agent, an anti-arteriosclerotic agent, an anti-allergic agent, a hypotensive agent, a wound healing agent, or an anti-inflammatory agent. The present invention is described in detail below. The hexapeptide represented by formulas (I) and (II), and pharmaceutically acceptable salts thereof are sometimes referred to below as the hexapeptide compound of the present invention. Amino acids used in this specification are also abbreviated according to a method employed in an IUPAC-IUB Committee of Biochemical Nomenclature (CBN) and are exemplified as follows: Arg: L-arginine His: L-histidine Ile: L-isoleucine Leu: L-leucine Lys: L-lysine Phe: L-phenylalanine Pro: L-proline Tyr: L-tyrosine Trp: L-tryptophan Val: L-valine D-Arg: D-arginine D-His: D-histidine D-Ile: D-isoleucine D-Leu: D-leucine D-Lys: D-lysine D-Phe: D-phenylalanine D-Pro: D-proline D-Tyr: C-tyrosine D-Trp: D-tryptophan D-Val: L-valine The six amino acids which constitute the hexapeptide of the present invention may either be of L-type or D-type. In the formulas (I) and (II), the first amino acid from the N terminus of the hexapeptide, represented by A, is an arginine or lysine whose N-terminal amino group is deaminated (Formula (I)), or alkylated or acylated (Formula (II)). As regards to the deamination of the N-terminal amino group of the amino acid A, when the deaminated arginine is prepared, for example, 5-aminovaleric acid is dissolved in a 2N-sodium hydroxide solution, then S-methylthiocarbamide is added to the solution, thereby obtaining the deaminated arginine. The alkyl group which is introduced to the N-terminal amino group of the amino acid A may be, e.g., methyl, ethyl, propyl and butyl. The alkylation of the N-terminal amino group may be carried out, for example, by reacting the amino acid A with a corresponding alkyl iodide such as methyl iodide or ethyl iodide in an organic solvent such as acetone. The acyl group which is introduced to the N-terminal amino group of the amino acid A may be, e.g., formyl, acetyl, propionyl, benzoyl or p-toluene sulfonyl. The acylation of the N-terminal amino group may be carried out, for example, by reacting the amino acid A with an acid anhydride such as acetic anhydride or an acid chloride such as acetyl chloride in an organic solvent such as methyl chloride or pyridine. In the formulas (I) and (II), the peptide bond between the fifth amino acid (represented by D) and the sixth amino acid (represented by E) from the N-terminus of the hexapeptide is represented by formula (III): where X represents a hydrogen atom or C 1 to C 4 alkyl group. In the formula (III), the alkyl group may be methyl, ethyl, n-propyl, t-propyl, n-butyl, i-butyl or t-butyl. In the formulas (I) and (II), the sixth amino acid from the N terminus of the hexapeptide, represented by E, is a valine, isoleucine or leucine. When the first amino acid A is deaminated, the C-terminal carboxyl group of the sixth amino acid E is substituted with, -CH 2 OR where R represents a hydrogen atom or a C 1 to C 4 alkyl group (Formula (I)). The alkyl group may be, e.g., methyl, ethyl, n-propyl, t-propyl, n-butyl, i-butyl and t-butyl. When the first amino acid A is alkylated or acylated, the C-terminal carboxyl group of the sixth amino acid E is substituted with -CH 2 OR as mentioned above (Formula (II)). An introduction of the substitution group to the C-terminal carboxyl group can be achieved, for example, by the alkylation as described above. The hexapeptides according to the present invention can be synthesized by a liquid- or solid-phase method which is a known method in peptide synthesis. A peptide synthesis using the solid-phase method will be described in detail below. In the solid-phase method, the six amino acids are sequentially condensed, using an organic solvent-insoluble resin, starting from the amino acid of the C-terminus of the hexapeptide and is then treated with an acid, thereby obtaining the hexapeptide in a free form. The organic solvent-insoluble resin is preferably chemically stable in an organic solvent and has good swell characteristic. The organic solvent-insoluble resin is exemplified by a resin obtained by introducing a side functional group such as a chloromethyl group or a hydroxymethyl group into a styrene-divinylbenzene copolymer. The functional group is activated prior to the synthesis of the hexapeptide. The carboxy group of the amino acid E is coupled to the activated functional group of the resin. In the solid-phase synthesis, only an α-amino group or both the α-amino group and a side-chain functional group of the amino acids which constitute the hexapeptide of the present invention is/are protected by a protective group, and the protected amino acids are used in the synthesis of the hexapeptide. Examples of the protective group of the α-amino group are a t-butyloxycarbonyl group (Boc), a 9-fluorenylmethyloxycarbonyl group (Fmoc), or their equivalent groups. Examples of a protective group for a phenolic hydroxyl group of tyrosine are a benzyl group (Bzl), a 2,6-dichlorobenzyl group (Cℓ 2 -Bzl) as a derivative thereof, an o-bromobenzyloxycarbonyl group (Br-Z), and their equivalent groups. Leucine and isoleucine can be generally used as protected by the Boc or Fmoc. These protected amino acids can be commercially available products. The synthesis of the hexapeptide according to the present invention will be described in more detail below. (1) Introduction of Constituent Amino Acid Prior to peptide synthesis, an organic solvent-insoluble resin (resin) as mentioned above must be activated. This activation can be performed as follows. The resin is placed in a peptide solid-phase method reaction vessel, and methylene chloride is added thereto. Then, a 10% triethylamine (TEA)/methylene chloride mixed solution is added thereto a total of one to three times. Every time the 10% TEA/methylene chloride is added, the resultant mixture is stirred for 5 to 10 minutes. The mixture is then filtered and the resin is washed. In this activated resin, TEA (amino group) is attached to the functional group of the resin. The reaction vessel as described above may have a charge port for charging a reagent including the protected amino acids, a solvent, and the like and a filter for filtering the solvent, and may allow a reaction of the resin and the reagent by shaking of the vessel or stirring of the contents. The reaction vessel is preferably made of glass or Teflon (Trade mark) and allows filtering by raising or reducing a pressure in the reaction vessel. About 2 to 20 mℓ of a solvent, such as methylene chloride, chloroform, dimethylformamide (DMF), or benzene, which can swell the resin are added to 1 g of the resin activated as described above to obtain a suspension. About 1 to 6 equivalent weight of a C-terminal amino acid, whose α-amino group is protected by the protective group with respect to one equivalent weight of the activated functional group of the resin, is added in the obtained suspension. The C-terminal amino acid corresponds to the sixth amino acid from the N-terminus of the hexapeptide of the present invention and is represented by E in the formulas (I) and (II). The resultant mixture is stirred or shaken for about 1 to 20 minutes. About 0.5 to 2 equivalent weight of a coupler which couples the C-terminal amino acid with the functional group of the resin is added to the suspension per equivalent weight of the C-terminal amino acid, and the suspension is stirred or shaken, thereby coupling the C-terminal carboxyl group of the C-terminal amino acid to the activated resin. Examples of the coupler are dicyclohexylcarbodiimide (DCC), water-soluble carbodiimide, carbonyldiimidazole, a Woodword's reagent "K", N-ethyl-2-hydroxybenzisoxazolium trifluoroborate, 1-ethoxycarbonyl-2-ethoxy-1-2-dihydroxyquinoline, a "Bop reagent", and diphenylphosphorylazido. The degree of progress of the coupling reaction can be monitored by a ninhydrin reagent or a fluorescamine test. When the reaction is not completed, coupling is repeated. Alternatively, the amino acid whose α-amino group is protected can also be combined to a resin in accordance with an active esterification method or a symmetry anhydride method, instead of the method using the coupler as described above. Upon completion of the reaction, the resin coupled with the C-terminal amino acid (C-terminal amino acid-resin) is washed once to several times by 2 to 50 mℓ of a washing solvent with respect to 1 g of the resin which is activated but not coupled with the amino acid, i.e., original activated resin. As the washing solvent, for example, at least one solvent of methylene chloride, chloroform, methanol, ethanol, DMF, benzene, and acetic acid can be used. The reaction product is then filtered. The nonreacted, activated functional groups or amino groups of the washed resin are then blocked prevent further reactions, using a terminating reagent and are then washed again. For example, about 0.5 to 5 equivalent weight of the terminating reagent with respect to 1 equivalent weight of the functional group of the resin is added and reacted with the resin for about 10 minutes to 18 hours. Examples of the terminating reagent are acetic anhydride/TEA/methylene chloride, acetic anhydride/TEA/chloroform, acetylimidazole/DMF, and fluorescamine/diisopropylethylamine/methylene chloride. Thereafter, the protective group of the α-amino group of the C-terminal amino acid-resin is eliminated for coupling with the next amino acid. For example, trifluoroacetic acid (TFA) is suitable as an elimination reagent for Boc. 100 v/v% TFA or TFA diluted to 10 v/v% or more with methylene chloride, chloroform, or their equivalents can be used, for example, as follows: About 2 to 50 mℓ of a TFA solution with respect to 1 g of the original activated resin are preferably added to the C-terminal amino acid-resin and reacted for about 5 to 60 minutes. After the reaction, the reaction mixture is filtered and the C-terminal amino acid-resin washed with the washing solvent. About 2 to 50 mℓ of a solution of about 5 to 30% TFA in methylene chloride, chloroform, or their equivalents are added with respect to 1 g of the original activated resin to the C-terminal amino acid resin, thereby neutralizing the residual TFA. Thereafter, the reaction product is washed with the washing solvent. Further, piperidine is suitable as an elimination reagent for Fmoc, and used upon dilution with methylene chloride, DMF, chloroform, or their equivalent to a concentration of 5 to 50%, for example, as follows: About 2 to 100 mℓ of the piperidine solution with respect to 1 g of original activated resin are added to the C-terminal amino acid-resin and reacted for about 5 to 60 minutes. After the reaction, the reaction solution is filtered and the C-terminal amino acid-resin washed with the washing solvent. Then, the fifth amino acid from the N-terminus of the hexapeptide of the present invention (represented by D in the formulas (I) and (II)) is coupled to the now free α-amino group of the C-terminal amino acid bonded to the resin, as follows. The solvent which can swell the resin mentioned above is added to the resultant C-terminal amino acid-resin to suspend it. About 1 to 6 equivalent weight of the protected fifth acid D with respect to 1-equivalent of the functional group on the resin is added to the suspension, and the coupler as described above is then added to the resultant mixture. The degree of progress of the coupling reaction can be monitored by a ninhydrin reagent or a fluorescamine test. After the reaction, the reaction product is washed with the washing solvent. When the reaction is not completed, the above coupling step is repeated or blocking of excessive α-amino groups of C-terminal amino acids from subsequent reactions is performed using the terminating reagent mentioned above. Thereafter, elimination of the protection group, washing, neutralization, washing, coupling, and washing are repeatedly performed in the subsequent steps following the same procedures as described above except that each protected amino acid corresponding to each constituent amino acid, is subsequently used, thereby successively condensing the forth amino acid (C) to the first amino acid A to the amino acid moiety on the resin. As a result, a peptide chain corresponding to the hexapeptide of the present invention can be obtained. (2) Elimination of Peptide Chain from Resin and Elimination of Protective Group The resin coupled with the peptide chain (i.e, peptide-resin) is treated with hydrogen fluoride (HF) to cut an amino bond between the peptide and the resin, and at the same time the protective group is eliminated to obtain a free peptide. A special vessel is required for the HF treatment, and is commercially available. More specifically, the dried peptide-resin is placed in a vessel, 0.5 to 5 mℓ of anisole are added thereto with respect to 1 g of peptide-resin to prevent a side reaction, and the mixture is stirred. 2 to 50 mℓ of a liquid HF are added to the mixture with respect to 1 g of the peptide-resin to treat the peptide-resin at -20 to 0°C for 0.5 to 2 hours. Herein, Dimethylsulfide or ethanedithiol is preferably added to the anisole. After the reaction, HF is removed at a reduced pressure, and residual HF, a protective group, anisole, and other additives are eliminated by a solvent such as ethyl acetate, diethyl ether, or benzene. The peptide is extracted using an aqueous acidic solution such as an aqueous acetic acid solution, and the resin from which the peptide is eliminated is removed by filtration. (3) Purification of Crude Peptide The extract obtained in the above step contains, in addition to the desired peptide, byproducts, such as defective peptides formed during the synthesis, and must be purified. More specifically, the extract containing the peptide and the byproducts is concentrated by ultrafiltration. Thereafter, ion exchange, freezing, and drying are performed, thereby obtaining a dried product. The dried product is purified by a fractional reverse-phase high-performance liquid chromatography. Finally, ion exchange and gel filtration of a fraction containing the desired peptide are performed to obtain a purified hexapeptide according to the present invention. Examples of a pharmaceutically acceptable, non-toxic salt of the hexapeptide according to the present invention are salts with an alkali metal such as sodium or potassium or with an alkaline earth metal such as calcium and magnesium, and acid addition salts with an inorganic acid such as hydrochloric acid, sulfuric acid, phosphoric acid or carbonic acid, or with an organic acid such as acetic acid, propionic acid, tartaric acid succicinic acid, malic acid, aspartic acid or glutamic acid. The pharmaceutically acceptable salt of the hexapeptide according to the present invention also includes a complex salt with a metal compound such as a zinc, a nickel or a cobalt compound, and with a polyamic acid such as poly-L-gultamic acid. Since the hexapeptide compound according to the present invention acts directly on vascular endothelial cells, it is effective and can strongly suppress an edema associated with the increasing vascular permeability. The hexapeptide compound can suppress edemata based on vascular endothelial disorders and various tissue disorders in addition to suppression of a cerebral edema. The hexapeptide compound according to the present invention also has an anti-endotoxin shock effect, protease inhibition action (an anti-thrombin action, an anti-plasmin action), a hyoptensive action, an anti-DIC action, an anti-allergic action, and a wound healing action. More particularly, the hexapeptide compounds according to the present invention can suppress increasing vascular permeability and do not exhibit any side effects in relation to steroids. Therefore, the hexapeptide compound is useful for diseases such as a cerebral edema, an edema of the lung, an edema of the trachea, a thrombus, an arteriosclerosis, a burn, and a hypertension, and allergic diseases such as a bronchial asthma and a pollinosis. More specifically, the hexapeptide compound according to the present invention is useful as an agent for reducing hemorrhage from a sharp trauma such as an injured tissue portion at the time of surgical operation, a lacerated wound of a brain or other tissues caused by a traffic accident and the like, and for relaxing and curing swelling, pain and inflammation caused by the traumas. The hexapeptide compound according to the present invention can also be useful for suppressing internal hemorrhage caused be a dull trauma, and edemata and inflammation which are accompanied with the internal hemorrhage. The hexapeptide compound according to the present invention also provides excellent effects in suppression and improvement of cerebral edemata by suppressing the leakage of blood components to the tissue matrix found in cerebral ischemetic diseases which include cerebral infractions (e.g., a cerebral thrombus and a cerebral embolism), intracranial hemorrhages (e.g., a cerebral hemorrhage and a subarachnoidal hemorrhage), a transient cerebral ischemic attack, and acute cerebral blood vessel disorders in a hypertensive encephalopathy. In addition, the hexapeptide compound according to the present invention provides effects in suppression and improvement of burns, chilblains, other skin inflammations and swelling, upper tracheal inflammation, asthma, nasal congestion, a pulmonary edema, and inflammable disorders caused by endogenous and exogenous factors, which directly damage vascular endothelia and mucous membranes, such as an environmental chemical substance, chemotherapeutics of cancer, an endotoxin, and an inflammation mediator. More specifically, the hexapeptide compound according to the present invention can reduce the hemorrhage, inflammation, swelling and pains of a sharp trauma, i.e., the tissue portion injured by a surgical operation and a traffic accident. The hexapeptide compound also suppresses internal hemorrhage caused by a dull trauma and edemate and inflammation which are accompanied with the internal hemorrhage. In addition, the hexapeptide compound according to the present invention is effective in treatments of cerebral ischemetic diseases which contain cerebral infarctions (e.g., a cerebral thrombus and a cerebral embolism), intracranial hemorrhages (e.g., a cerebral hemorrhage and a subarachnoidal hemorrhage), a transient cerebral ischemic attack, and acute cerebral blood vessel disorders in a hypertensive encephalopathy. In particular, since cerebral edema are produced during these acute cerebral blood vessel disorders, suppression of edemata greatly influences the recovery after curing the disease. The pharmaceutical agent according to the invention, containing a hexapeptide compound as an active ingredient, can be used as a medicine in therapy of the above-mentioned diseases. The pharmacetical agent of the invention is especially good for an anti-edematous agent, an anti-shock agent, an anti-thrombus agent, an anti-arteriosclerosis agent, an anti-allergic agent, a hypotensive agent, a wound healing agent, and an anti-inflammatory agent. The hexapeptide compound according to the invention and the pharmaceutical agents can be administered either orally or parenterally in an amount effective for therapy. For adults, the effective amount of the hexapeptide compound of the invention per day is between 0.1 and 150 nmol/kg. Within this range, the amount of the hexapeptide compound administered should be determined depending on a variety of factors such as the degree of the disorder, the weight of the patient, and age. The pharmacetical agent of the invention may contain a pharmaceutically acceptable diluent or excipient, in the form of a liquid, gel, or solid, other than the hexapeptide compounds. Further, if needed, the agent may contain an additive which can be generally used in a drug composition, such as a general antiseptic agent, anti-oxidation agent, or the like. The pharmaceutical agent of the invention can be used as an oral or parenteral administration drug. The oral administration drug takes the form of, for example, a regular tablet, capsule, powder, solution, or suspension, whereas the parenteral administration drug is in the form of, for example, a regular solution injection, suspension injection, suppository, or nasal mucosal spray. It should be noted that the agent is preferably administered through intravenous or hypodermic injection. Further, the form of the agent can be selected depending on the condition of the patient, age, and the degree of the disorder. The present invention will be described in more detail by way of its examples. However, the present invention is not limited by these illustrative examples. Examples 1, 13 and 15 of the following examples fall outside the scope of the present invention, but are included for comparison purposes. Firstly, examples of the synthesis of the hexapeptide according to the present invention will be described below. In the examples, the hexapeptide numbers correspond to the hexapeptides shown in Tables 1 and 2. In Table 1 and 2, desamino-Arg represents ω-guanidinopentanic acid. N-acetyl- and N-butyl- represent acetylated and butylated derivatives of a N-terminal amino acid of the hexapeptide, respectively. -OH represents that a carboxylic group of a C-terminal amino acid is free, and -NH 2 represents that the carboxylic group is converted into a carbamoyl. Leucinol is an alcohol form of leucine, i.e. a -CH 2 OH group replaces the carboxylic group of leucine. Peptide No. Formula SEQ ID No: 1 Desamino-Arg-Arg-Pro-Tyr-Ile-Leu-OH 1 Peptide No. Formula SEQ ID NO: 2 Desamino-Arg-Arg-Pro-Tyr-Ile-Leucinol 2 3 Desamino-Arg-D-Arg-Pro-Tyr-Ile-Leucinol 4 Desamino-Arg-D-Lys-Pro-Trp-Ile-Leucinol 5 Desamino-Arg-Arg-Pro-Trp-Ile-Leucinol 5 6 Desamino-Arg-Arg-Pro-D-Trp-Ile-Leucinol 7 Desamino-Arg-Arg-Pro-Tyr-Ile-(NCH 3 )Leucinol 7 8 Desamino-Arg-D-Lys-Pro-Trp-Ile-Leucinol 9 Desamino-Arg-D-Lys-Pro-Tyr-Ile-(NCH 3 )Leucinol 10 Desamino-Arg-Arg-Pro-Trp-Ile-(NCH 3 )Leucinol 10 11 Desamino-Arg-D-Lys-Pro-Trp-Ile-(NCH 3 )Leucinol 12 N-acetyl-Arg-Arg-Pro-Tyr-Ile-Leucinol 12 13 N-acetyl-Arg-Arg-Pro-Tyr-Ile-Leu-NH 2 13 14 N-buthyl-Arg-Arg-Pro-Tyr-Ile-Leucinol 14 15 N-buthyl-Arg-Arg-Pro-Tyr-Ile-Leu-NH 2 15 Example 1 (Synthesis of Peptide No. 1) Pretreatment; Activation of Resin 150 g of an organic solvent-insoluble resin (available from Peninsula Lab.; 1% divinylbenzene; 100 to 200 mesh) was placed in a peptide solid-phase synthesis reaction vessel (available from Peninsula Lab.), and the following solvents were added twice each for 5 minutes and stirred and the solvents filtered off, to obtain an activated resin. (i) Methylene chloride: 1 ℓ (ii) 10% TEA/methylene chloride: 420 mℓ As a washing operation, 1.5 ℓ of methylene chloride, 1.5 ℓ of methanol, and 1.5 ℓ of methylene chloride were added to the activated resin in the order named, and stirred for 2 minutes. The above operation was repeated twice, and the resultant solution was filtered off (this washing operation will be referred to as washing operation I hereinafter). Step 1; Coupling Amino acid to Resin The following materials were added in turn to the whole of the resin obtained in the pretreatment and was stirred, and the resultant mixture was filtered. (1) DMF: 1.5 ℓ (2) Boc-Leu·H 2 O: 38.2 g (3) 1M DCC/DMF-CH 2 Cℓ 2 : 120 mℓ (stirring for 20.0 hours) Herein, Boc- represents that the α-amino group of an amino acid is protected by a protective group Boc. Subsequently, the washing operation I was performed. The result of a ninhydrin test on the treated resin was negative. Step 2; Elimination of Protective group The following solvent was added to the whole of the resin obtained in step 1, and the mixture was stirred and filtered. 50% TFA/methylene chloride (stirring for 5 and 25 minutes, each for 1.8 ℓ) Subsequently, the washing operation I was performed. Steps 3 - 7; Extension of Peptide chain To the whole of the resin obtained in step 2, coupling of amino acids, elimination of protective groups, neutralization, and washing were performed following the same procedures as in steps 1 and 2 except that amino acids protected by protective groups (protected amino acids) shown in Table 3 below were sequentially coupled to the Leu on the resin using coupler under coupling conditions shown in Table 3. In Table 3, HOBt represents 1-hydroxybenzotriazole. Step Protected Amino Acid Coupling Condition Coupler Amount Used (g) Solvent Time (h) 3 Boc-Ile·1/2H 2 O 72.0 CH 2 Cℓ 2 16.0 DCC 4 Bod-Tyr(Br-Z) (1)237.2 CH2 C ℓ 2 17.0 DCC (2)100.0 DMF 44.0 DCC+HOBt 5 Boc-Pro (1) 64.5 DMF 13.5 DCC+HOBt (2) 25.0 CH 2 Cℓ 2 3.0 DCC 6 Boc-Arg(Tos) 43.0 CH 2 Cℓ 2 20.0 DCC 7 Desamino-Arg (Tos) 43.0 CH 2 Cℓ 2 20.0 DCC After coupling of Desamino-Arg and washing in step 7 were completed, a peptide-resin was removed from the reaction vessel and was dried. Deamination of Arg used in step 7 was performed as follows. First, 0.1 mol of 5-aminovaleric acid hydrochloride was dissolved in 50 mℓ of a 2N-sodium hydroxide solution. 0.1 mol of S-methylthiocarbamide was added thereto, and the resultant solution was stirred and reacted at room temperature for 2 days. The reaction mixture was concentrated and purified by column chromatography. The purified product was converted to a hydrochloride. The Desamino-Arg hydrochloride obtained as described above was used in step 7. Step 8; Elimination of Peptide from Resin and Elimination of Protective Group 4 g of the peptide-resin obtained in step 7 was placed in a HF reaction vessel, and 4 mℓ of anisole and 1 mℓ of methylsulfide were added thereto and stirred. Thereafter, the HF reaction vessel set in an HF reaction apparatus (available from Peptide Lab.) The HF reaction vessel was cooled in a dry ice-acetone bath, and about 35 mℓ of HF were charged thereto. The contents of the vessel was stirred and reacted while cooling in an ice water bath for 45 minutes. After completion of the reaction, HF was distilled off by a vacuum pump for 15 minutes. After the HF reaction vessel was removed from the ice water bath to a water bath, evacuation of HF was continued for 15 minutes. Ether was added to the solution, and the resultant solution was stirred well and filtered using a glass filter. The product remaining on the filter was washed with ether (a total of about 300 mℓ), and a peptide eliminated from the resin was extracted four times using 100 mℓ of a 0.1N aqueous acetic acid solution. Step 9; Purification The pH of the extract obtained in step 8 was adjusted to 5.0 by acetic anhydride, and filtered using an ultrafiltration membrane having a molecular weight of 1,000. The resultant solution was subjected to a column of a cation exchange resin (available from Whatman). Using an ammonium acetate buffer as a mobile phase, the component absorbed on the resin was eluted. The eluted component was freeze-dried. The dried product was purified by a fractional reverse-phase high-performance liquid chromatography. In this chromatography, use was made of an ODP-90 (available from ASAHI CHEMICAL INDUSTRY CO., LTD.) as the column and a phosphate buffer/acetonitrile mixed solvent as the gradient eluent. A main peak fraction obtained by the above chromatography was concentrated by the ultrafiltration membrane as mentioned above. The obtained concentrate was subjected to a column of cation exchange resin SP-Sephadex (available from Pharmacia Fine Chemicals, Inc.). Using an aqueous sodium chloride solution as a mobile phase, the component adsorbed on the resin was eluted. The eluted component was subjected again to a column of Sephadex G-25 (available from Pharmacia Fine Chemicals, Inc.). Thereafter, using an aqueous acetic acid solution as a mobile phase, the component adsorbed on the resin was eluted, and was freeze-dried. The resultant product exhibited a single spot by a thin layer chromatography using three developing solvents and electromigration (Whatman 3MM, pH 3.5; 1,500 V. 1hr.). The amino acid analysis of the purified product is summarized as follows. Values within the parentheses represent theoretical values. Example 1 (Peptide No. 1) Arg: 0.89 (1); Pro: 1.02 (1); Tyr: 0.98 (1) ; Ile: 0.97 (1); Leu: 1.01 (1); Desamino-Arg NH 3 : - (1) The result of the amino acid analysis shows that the purified product obtained by the above method was the peptide No. 1 having the amino acid composition shown in Table 1 above. Examples 2 - 11 (Synthesis of Peptides Nos. 2 - 11) Five amino acids corresponding to each of peptides Nos. 2 to 11, other than leucinol, were sequentially condensed by the same procedures as in Example 1. The synthesized peptide chains each consisting of the five amino acids were eliminated from the resins according to the same procedures as in Example 1. In order to protect an α-amino group of an N-terminal amino acid of each peptide chain by Boc, each peptide chain (0.02 mol) was dissolved in a solvent mixture of 30 mℓ of water and 30 mℓ of dioxane, and sodium bicarbonate (0.048 mol) and Boc-N 3 (0.024 mol) were added thereto. The resultant solution was stirred and reacted at 40 to 45°C for 24 hours. After the reaction, the reaction mixture was concentrated at a reduced pressure, 50 mℓ of water was added thereto, and the resultant mixture was washed with 50 mℓ of ethyl acetate. The water layer was collected, and 70 mℓ of 0.5M citric acid was added thereto with icing. Sodium chloride was added to the resultant solution to be saturated. The reaction product was extracted with 100 mℓ ethyl acetate three times and was then dried with sodium sulfate. The dried product was concentrated at a reduced pressure. The peptide chains (0.005 mol) were dissolved in 10 mℓ of THF, and 0.45 mℓ of N-methylformate and 0.35 mℓ of ethyl chlorocarbonate were added thereto while the solution was cooled to -20°C. After a lapse of 5 minutes, 5 mℓ of a DMF solution containing leucinol hydrochloride (0.005 mol) and N-methylformate (0.45 mℓ) were added to the above solution, and the resultant mixture was stirred and reacted at -70°C for 2 hours. As result, leucinol was condensed to the peptide chain. After the reaction, the precipitant was filtered off, and the filtrate was concentrated, dried, and solidified. Thereafter, 50 mℓ of ethyl acetate was added to the solid body to dissolve the solid body, and the mixture was sequentially washed with 50 mℓ of 5% sodium bicarbonate two times and 50 mℓ of distilled water three times. The washed ethyl acetate ester layer was concentrated and solidified, and the protective group was eliminated by the same procedure as in Example 1. The crude product thus obtained was purified by HPLC to obtain each desired hexapeptide. Results of amino acid analysis of the purified products are shown below. Values within the parentheses represent theoretical values. Example 2 (Peptide No. 2) Arg: 2.02 (2); Pro 0.97 (1); Tyr: 1.02 (1); Ile: 0.98 (1); Leucinol: - (1) Example 3 (Peptide No. 3) Arg: 1.98 (2); Pro: 1.04 (1); Tyr: 0.96 (1); Ile: 1.02 (1); Leucinol: - (1) Example 4 (Peptide No. 4) Arg: 0.95 (1); Lys: 0.97 (1); Pro: 0.99 (1); Tyr: 0.96 (1); Ile: 1.02 (1); Leucinol: - (1) Example 5 (Peptide No. 5) Arg: 2.05 (2); Pro: 0.98 (1); Trp: 1.02 (1); Ile: 0.97 (1); Leucinol: - (1) Example 6 (Peptide No. 6) Arg: 1.97 (2); Pro: 1.03 (1); Trp: 0.98 (1); Ile: 1.02 (1); Leucinol: - (1) Example 7 (Peptide No. 7) Arg: 2.04 (2); Pro: 0.98 (1); Tyr: 1.02 (1); Ile: 0.96 (1); Leucinol: - (1); NCH 3 : present Example 8 (Peptide No. 8) Arg: 1.04 (1); Lys: 1.03 (1); Pro: 0.97 (1); Trp: 0.98 (1); Ile: 0.97 (1); Leucinol: - (1) Example 9 (Peptide No. 9) Arg: 1.02 (1); Lys: 0.98 (1); Pro: 1.02 (1); Tyr: 1.01 (1); Ile: 1.05 (1); Leucinol: - (1); NCH 3 : present Example 10 (Peptide No. 10) Arg: 2.03 (2); Pro: 1.02 (1); Trp: 0.98 (1); Ile: 0.96 (1); Leucinol: - (1) Example 11 (Peptide No. 11) Arg: 1.02 (1); Lys: 0.97 (1); Pro: 0.96 (1); Trp: 1.01 (1); Ile: 0.97 (1); Leucinol: - (1); NCH 3 : present Herein, "NCH 3 " represents a peptide bond having a hydrogen atom substituted by a methyl group. These results of amino acid analysis confirmed that the purified products obtained by the above method were peptides Nos. 2 to 11 each having the amino acid composition shown in Table 2. Example 12 (Peptide No. 12) Five amino acids corresponding to peptide No. 12, other than leucinol, were sequentially condensed on the resin by the synthesis method following the same procedures as in Example 2. Thereafter, an acetyl group was introduced into an N-α-amino group of the peptide chains thus obtained, by a known method. The synthesized peptide chain was eliminated from the resin, and the protective group was eliminated from the peptide chain as in Example 1. The resin was washed with a washing solvent. The washing solution and the filtrate were concentrated and the concentrate obtained was freeze-dried. The α-amino group of the peptide chain contained in the dried product was protected with Boc. Leucinol was then condensed to the peptide chain, and the protective group was eliminated from the hexapeptide thus obtained. The crude product was purified with HPLC. A result of amino acid analysis of this purified product is shown below. Values within parentheses represent theoretical values. Arg: 1.99 (2); Pro: 1.02 (1); Tyr: 0.91 (1); Ile: 0.99 (1); Leucinol: - (1) Mass analysis of this hexapeptide was performed by a fast atom bombardment mass spectrometry (FAB). As a result, a molecular ions peak of m/z=846 was observed, thereby confirming that synthesis of the target hexapeptide was completed. Example 13 (Peptide No. 13) Using a benzohydrylamine (BHA) resin support or a paramethylbenzohydrylamine (MBHA) resin support, amino acids corresponding to peptide No. 13 were sequentially condensed in the resin following the same procedures as in Example 1. Upon completion of the synthesis, the N-terminal amino acid was acetylated, the peptide chain thus obtained was eliminated from the resins, and the protective group was eliminated from the peptide chain. A result of amino acid analysis of this purified product is shown below. Values within the parentheses represent theoretical values. Arg: 2.02 (2); Pro: 0.99 (1); Tyr: 1.03 (1); Ile: 0.97 (1); Leu: 1.06 (1); NH 3 ; 1.12 (1) The result confirmed that the resultant product obtained by the above method was peptide No. 13 having the amino acid composition shown in Table 2. Example 14 (Peptide No. 14) Five amino acids corresponding to the peptide No. 14, other than leucinol, were sequentially condensed by the synthesis method following the same procedures as in Example 12, and a butyl group was introduced into an N-α-amino group of the N-terminal amino acid by a known method using butyl chloride. Leucinol was condensed by the method following the same procedures as in Example 12, the protective group was eliminated from the peptide chain thus obtained, and the crude product containing the hexapeptide was purified with HPLC. A result of amino acid analysis of this purified product is shown below. Values within the parentheses represent theoretical values. Arg: 1.97 (2); Pro: 0.96 (1); Tyr: 1.04 (1); Ile: 0.98 (1); Leucinol: - (1) The result confirmed that the resultant product obtained by the above method was peptide No. 14 having the amino acid composition in Table 2. Example 15 (Peptide No. 15) Amino acids corresponding to the peptide No. 15 were sequentially condensed following the same procedures as in Example 13. Upon completion of the synthesis, a butyl group was introduced to an N-terminal amino acid by a known method. The obtained peptide chain was eliminated from the resin, and the protective group was eliminated from the peptide chain in accordance with the method following same procedures as in Examples 13. The crude product containing the hexapeptide was purified with HPLC. A result of amino acid analysis of this purified product is shown below. Values within the parentheses represent theoretical values. Arg: 2.03 (2); Pro: 0.98 (1); Tyr: 0.94 (1); Ile: 0.98 (1); Leu: 1.01 (1); NH 3 : 1.09 (1) Mass analysis of this peptide was performed by FAB, and molecular ions peak of m/z = 871.5 was observed, thereby confirming that synthesis of the desired hexapeptide was completed. Pharmacological Effect Test In order to confirm an increasing vascular permeability suppression action, a vascular endotherial disorder amelioration action, an anti-edematous action, an anti-inflammatory action, an anti-shock action, an anti-DIC action, a protease inhibition action, an anti-allergic action, a hypotensive action, and a wound healing action of the hexapeptide compound according to the present invention, the following experimental examples 1 to 8 were performed. Experimental Example 1: Vascular Permeability Acceleration Suppression Action After 6-week male Sprague-Dawley (SD) rats (each group consisted of 10 rats) were fed for a week, they were used in this experiment. The concentrations of samples of the hexapeptides (peptide Nos. 1 to 11) were dissolved by an acetic acid buffer solution added with a 1% bovine serum albumin (BSA). An aliqrot of 1 mℓ/kg of each of the peptides Nos. 1 to 11 was injected into the femoral vein of the right leg of each rat anesthetized with 50 mg/kg of Nembutal Sodium (available from Dainippon Pharmaceutical Co., Ltd.). After 10 minutes, the nontreated left leg of the rat was dipped in 58°C hot water for one minute to form an edema. Each rat was left at room temperature for 29 minutes, and the volumes of both legs of each rat were measured. As a control, the experiment following the same procedure as described above was performed except that 1 mℓ/kg of a sodium acetate buffered solution containing 1% BSA, instead of the hexapeptide of the present invention, was injected in rats. A rate (%) of increase in leg volume was calculated from the result of the above measurement by equation (1) below: Rate of Increase in Leg Volume (%) = (Heated Leg Volume) - (Non-heated Leg Volume) (Heated Leg Volume) × 100 The rates were compared with that of the control. The results are summarized as suppression rates (%) obtained by equation (2) shown in Table 4 As shown in Table 4, the peptides Nos. 1 to 11 were confirmed to have a vascular permeability acceleration suppression action and could suppress edema. An action of the hexapeptide compounds according to the present invention (peptide Nos. 2 to 11) for suppressing the increasing vascular permeability is a lowering of the permeability of vascular endothelial cells to close a gap between the vascular endothelial cells. In arteriosclerotic diseases as shown in a hypercholesterolemia model of a monkey proposed by R. Ross (pp. 103 - 108, 1990, Elsevier Science Publisher B.V., Netherlands), monocytes, T-lymphocytes, and denatured cholesterols are attached to the gaps between the vascular endothelial cells and intrude under the vascular endothelial cells through the gaps, thereby finally forming fatty spots as an initial change in morbid state in an arteriosclerosis. The vascular endothelial cells are thinned with an increase in fatty spots and are finally separated and open. Thereafter, most of foamy cells flow out, and platelets are attached to cause a thrombus. Smooth muscle cells are grown by a PDGF (Platelet-Derived Growth Factor) produced from the platelets, and the wall thickness of the artery is increased to result in an arteriosclerosis. When the gap between the vascular endothelial cells is reduced by a hexapeptide according to the present invention, invasion of a low-density lipoprotein (LDL) and monocytes below the vascular endothelial cells can be prevented. Hence, arteriosclerosis can be prevented. Experimental Example 2: Anti-Shock Action In this experiment, male ICR mice (each group consisted of 10 mice) each having a weight of 25 g to 35 g were used. Each of peptides Nos. 1, 12 and 13 was dissolved in a sodium acetate buffered solution (pH 6.0) with 1% BSA added thereto or in a physiological saline. An endotoxin to induce a shock was obtained by dissolving Lipopolysaccharide [E.coli; 0127B8 (Difco)] in a physiological saline. Each of peptides Nos. 1, 12 and 13 was intravenously injected at a dose of 5 mℓ/kg to each mouse, and the endotoxin with a concentration of 10 mg/kg was intravenously injected at a dose of 5 mℓ/kg after 10 minutes. The lethal rates (%) of these mice within 24 hours were measured. As a control, 5 mℓ/kg of the sodium acetate buffered solution with 1% BSA added thereto was injected instead of the hexapeptide of the present invention. The results are summarized in Table 5. Peptide No. Dose Lethal rate within 24 hours (%) Control ------- 80 1 32n mol/kg × 4 20 12 32n mol/kg × 4 10 13 32n mol/kg × 4 20 In this experiment, a septic shock is a major cause of death of the mouse. The septic shock is an acute circulatory disorder which exhibits a shock state such that a vascular endothelial disorder occurs by bacterial infection by means of an endotoxin serving as a bacterial cell wall component, and increasing vascular permeability to cause leakage of blood components outside the blood vessel. Thus, as shown in Table 5, it is apparent that peptide Nos. 1, 12 and 13 can suppress the increasing vascular permeability, and it was confirmed that they are useful as anti-shock drugs. Experimental Example 3: Anti-Edematous Action In this experiment, 8-week male Wistar rats were used, and peptides Nos. 1, 12 and 13 dissolved in a saline added with 1% BSA were used. Iron column whose diameter was 5 mm cooled by dry ice acetone was placed on the left epidural portion of each rat to cause a vascular cerebral edema. Freezing was performed to form an injured portion after an hour, and the brain of each rat was taken out. The brain was divided into right and left cerebral cortices, and weights of the left cerebral cortices were measured. The left cerebral cortices were dried at 105°C for 24 hours, and weights of the dried left cerebral cortices were measured to obtain water contents (%). In this operation, the peptides Nos. 1, 12 and 13 were intravenously injected at the concentration shown in Table 6 to each rat 10 minutes before and 20 minutes after the freezing. As a control, 1 mℓ/kg of the saline with 1% BSA added thereto, instead of the peptides, was injected into the rat according to the same procedures as described above. The results are summarized in Table 6. Peptide No. Dose Water Content (%) Control ------ 81.4 1 32n mol/kg 81.2 12 32n mol/kg 80.8 13 32n mol/kg 80.6 As shown in Table 6, peptide Nos. 1, 12 and 13 were confirmed to have the increasing vascular permeability suppression action and suppress the edema. Experimental Example 4: Hypotensive Action Hybrid grown-up dogs (each group consisted of six dogs) were used in this experiment. Peptide No. 1 was dissolved in a sodium acetate buffered solution with a 1% BSA added thereto. 25 mg/kg of sodium pentobarbital was intravenously injected to each dog. The blood pressure of the femoral artery and blood flow rate of the ascending aorta of each dog were measured while performing the artificial respiration. 2 nmol/kg of peptide No. 1 was intravenously injected. Thereafter, the blood pressure and the blood flow were measured again. As a result, for peptide No. 1, although the average blood pressure before the hexapeptide injection was 84 mmHg, the average blood pressure after the peptide injection was decreased to 48 mmHg. The blood flow rate was decreased from 0.95 ℓ/min to 0.75 ℓ/min by the injection. The hexapeptide compounds according to the present invention have a hypotensive action and can serve as useful hypotensive agents by reducing the resistance in peripheral blood vessels. Experimental Example 5: Anti-DIC Action Hybrid grown-up dogs (each group consisted of six dogs) were used in this experiment. 30 mg/kg of pentobarbital was intravenously injected to anesthetize each dog. Intravenous injection of 32 nmol/kg of each of peptides Nos. 1, 12 and 13 was performed. After 30 minutes, 3 mg/kg of an endotoxin derived from Escherichia Coli was intravenously injected while the artificial respiration was performed with air. Samples of blood were collected from each dog immediately before the injection of the peptides and 120 minutes after the injection of the endotoxin. As a control, a experiment was performed according to same procedure as described above except that the hexapeptide was not injected. The results are summarized in Table 7. The number of platelets is the number per 1 mm 3 (1 µℓ) of the blood in Table 7. Peptide No. Average Number of Platelets Immediately before Injection of Endotoxin Average Number of Platelets 120 Minutes after Venous Injection of Endotoxin Control 72 × 10 4 43 × 10 4 1 68 × 10 4 61 × 10 4 12 70 × 10 4 64 × 10 4 13 71 × 10 4 58 × 10 4 As is apparent from the above results, the hexapeptide according to the present invention was confirmed to suppress a blood coagulation acceleration state and a decrease in the number of platelets. Experimental Example 6: Protease Inhibition Action Each of peptide Nos. 1, 12 to 15 was mixed at various volumes with thrombin (final conc. 10 U/mℓ), and the resultant mixture was preincubated at 37°C for 5 minutes. A synthetic coloring base (Test Team R S-2238 available from Daiichi Kagaku Yakuhin) was added to the above preincubated mixture and was reacted at 37°C for 15 minutes. 2% citric acid was added to the above mixture to stop the reaction. Hydrolysis was performed using residual thrombin, and free p-nitroaniline removed from the synthetic coloring base was subjected to colorimetric determination at a wavelength of 405 nm, thereby obtaining the inhibition capability with reference to 0.1M tris buffer. On the base of the inhibition capability thus obtained, a concentration of the peptides when each peptide inhibited the activity of thrombin with 50%, i.e. IC 50 was calculated. The results are summarized in Table 8. Peptide No. IC 50 Value (µM) 1 2.5 12 2.3 13 2.2 14 2.3 15 2.2 As is apparent from the above results, the hexapeptide compounds according to the present invention were confirmed to have an anti-thrombin action as an inhibition action against a protease. Experimental Example 7: Anti-allergic Action In this experiment, 6-week, male Wistar rats (each group consisted of 10 rats) were used, and the backs of these rats were shaved. An anti-egg albumin rat serum was diluted with saline to forty-fold of its volume, thereby obtaining an anti-egg albumin rat serum solution. 1 mℓ of the anti-egg albumin rat serum solution was intradermally injected to both side parts against the median line in the shaved portion, thereby passively sensitizing the rats. After 48 hours, 1 mℓ of a 0.5% Evans blue solution containing 10 mg of a egg-albumin was intravenously injected to the each rat, thereby provocating a passive cutaneous anaphylaxis (PCA) reaction. After 30 minutes, the rats were decapitated, thereby being subjected to hemorrhage and death. The skins were peeled from the rats, and a long diameter and a short diameter of blue stained portions caused by the PCA reaction in each rat were measured, and area of the blue-stained portions were calculated on the base of the obtained data. In these operations, each of peptide Nos. 1, 31 and 32 was dissolved in a sodium acetate buffered solution containing 1% BSA and was intravenously injected to each rat 10 minutes before the provocation of the PCA reaction. As a control, the sodium acetate buffer solution containing 1% BSA instead of the peptide solution was injected according to same procedure as described above. The results are summarized in Table 9. Peptide No. Dose Area of blue-stained portion (mm 2 ) Control -------- 188. 3±9.71 1 32 nmolg/kg 80.2±3.76 12 32 nmolg/kg 141.5±7.96 13 32 nmolg/kg 112.8±4.83 As shown in Table 9, the hexapeptide compound according to the present invention was confirmed to have an anti-allergic action, and was useful for an anti-allergic agent. Also, hexapeptide compounds according to the present invention are useful as anti-inflammatory agents, since the inflammation originates in the disorders of the vascular endothelium and the mucous membranes caused by e.g., various parameters, which were eliminated by the increasing vascular permeability suppression. Experimental Example 8: Wound Healing Action The male SD rats (each group consisted of 10 rats) were anesthetized with ether and the back of each rat was shaved, and the anesthetized portion was formed by a length of 30 mm along the median line by using a degreased, sterilized razor blade. The wound was immediately sutured at three equidistant positions, and the stitches were taken out after three days. The rats were subjected to hemorrhage and death on the eighth day, and the skins were peeled from the rats. A skin stripe having a size of 1 cm × 4 cm was formed from a wounded portion of each rat. Both ends of this stripe were set in a wound curing measurement tensile test instrument (TK-251 available from Unicom), and the tensile force (traction tension: g/cm) required to cut the wound portion was measured. Each hexapeptide compound was dissolved in an ace tic acid buffer solution containing 1% BSA and was intravenously injected to each rat for 8 days (once a day) from the date of wound formation. As a control, 1 ml/kg of the sodium acetate buffered solution containing 1% BSA instead of the hexapeptide solution was injected according to the same procedure as described above. The results are summarised in Table 10. Peptide No. Dose (nmol/kg) Traction Tension (g/cm) Control -- 456.2±21.6 1 32 545.7±31.2 12 32 539.2±27.7 13 32 547.0±32.3 As is apparent from the above results, the hexapeptide compound of the invention was found to promote wound healing of the skins of the rats. Toxicity Test An acute toxicity test of peptide Nos. 1, 12 and 13 was performed by using ddY mice. No mice died following intravenous injection with 2,000 times (64 µmol/kg) the effective amount. No toxic expression was therefore observed. Various pharmaceutical agents containing peptide compounds as the active ingredient will be described below. These agents do not fall within the scope of the present invention, as the active ingredient is not as defined in accordance with the present invention. However, the following pharmaceutical agents illustrate how peptide compounds of the invention can be formulated as pharmaceutical agents. The peptides given in Examples 17 to 26 can be synthesized and purified by following the same procedures as described in Example 1, except that the starting amino acids are changed. Example 16 Peptide No. 1 obtained in Example 1, injection distilled water, sodium chloride, and gelatin were used to obtain an injection in accordance with a conventional injection manufacturing method. Example 17 Desamino-Arg-D-Arg-Pro-Tyr-Ile-Leu-OH, injection distilled water, sodium chloride, sodium acetate, benzyl alcohol, and gelatin were used to obtain an injection in accordance with a conventional injection manufacturing method. Example 18 Desamino-Arg-D-Lys-Pro-Tyr-Ile-Leu-OH, injection distilled water, sodium chloride, sodium acetate, gelatin, and phenol were used to obtain an injection in accordance with a conventional injection manufacturing method. Example 19 Desamino-Arg-Arg-Pro-Trp-Ile-Leu-OH, injection distilled water, sodium chloride, sodium acetate, methyl para-hydroxybenzoate, ethyl para-hydroxybenzoate, propyl para-hydroxybenzonate, and butyl para-hydroxybenzonate were used to obtain an injection in accordance with a conventional injection manufacturing method. Example 20 Desamino-Arg-Arg-Pro-D-Trp-Ile-Leu-OH, sodium chloride, sodium acetate, hydrochloric acid, methyl para-hydroxybenzoate, ethyl para-hydroxybenzoate, propyl para-hydroxybenzoate, and butyl para-hydroxybenzoate were used to obtain an injection in accordance with a conventional injection manufacturing method. Example 21 An aqueous solution containing Desamino-Arg-Arg-Pro-Tyr-Ile-(NCH 3 )Leu-OH and mannitol was freeze-dried. An aqueous solution containing gelatin and phenol was added to the dried product to obtain an injection. Example 22 An aqueous solution containing Desamino-Arg-D-Lys-Pro-Trp-Ile-Leu-OH, sodium acetate, and human albumin was freeze-dried. Injection distilled water was added to the dried product to obtain an injection. Example 23 Desamino-Arg-D-Lys-Pro-Tyr-Ile-(NCH 3 )Leu-OH and cacao butter or Whitepsole were used to obtain a suppository in accordance with a conventional suppository manufacturing method. Example 24 An aqueous solution containing Desamino-Arg-Arg-Pro-Trp-Ile-(NCH 3 )Leu-OH, glacial acetic acid, sodium acetate, and benzalkonium chloride was sprayed into a nasal cavity with an intranasal spray applicator to obtain a drug applied to nasal mucous membranes. Example 25 An aqueous solution containing Desamino-Arg-D-Lys-Pro-Trp-Ile-(NCH 3 )Leu-OH, glacial ace tic acid, sodium acetate, and bile acid salt was sprayed into a nasal cavity with an intranasal spray applicator to obtain a drug applied to nasal mucous membranes. Example 26 Desamino-Arg-Arg-Pro-Tyr-Ile-Leu-OEt and gavakisate methanesulfonate were used to form a capsule in accordance with a conventional capsule manufacturing method.

Claims: A hexapeptide of the formula I: A-B-Pro-C-D-E where A is the L- or D- form of arginine or lysine, the N-terminal amino group of which is deaminated, B is the L- or D- form of arginine, lysine or histidine, Pro is the L- or D- form of proline, C is the L- or D- form of tyrosine, tryptophan or phenylalanine, D is the L- or D- form of valine, isoleucine or leucine, and E is the L- or D- form of valine, isoleucine or leucine, one of the hydrogen atoms of the amino group of which may be replaced by a C 1 to C 4 alkyl group and the C- terminal carboxyl group of which is replaced by -CH 2 OR wherein R is a hydrogen atom or a C 1 to C 4 alkyl group; or a pharmaceutically acceptable salt of the hexapeptide. A hexapeptide of the formula (II) A-B-Pro-C-D-E where A is the L- or D- form of arginine or lysine, the N-terminal amino group of which is alkylated or acylated, B is the L- or D- form of arginine, lysine or histidine, Pro is the L- or D- form of proline, C is the L- or D- form of tyrosine, tryptophan or phenylalanine, D is the L- or D-form of valine, isoleucine or leucine, and E is the L- or D-form of valine, isoleucine or leucine, one of the hydrogen atoms of the amino group of which is replaced by a C 1 to C 4 alkyl group and the C- terminal carboxyl group of which is replaced by -CH 2 OR wherein R is a hydrogen atom or a C 1 to C 4 alkyl group; or a pharmaceutically acceptable salt of the hexapeptide. A pharmaceutical composition which comprises, as an active ingredient thereof a hexapeptide of formula (I) specified in claim 1 or formula (II) specified in claim 2, or a pharmaceutically acceptable salt thereof. A pharmaceutical composition according to claim 3, further comprising a pharmaceutically acceptable carrier. A pharmaceutical composition according to claim 3 or 4, which is an anti-edematous agent, an anti-shock agent, an anti-thrombus agent, an anti-arteriosclerotic agent, an anti-allergic agent, a hypotensive agent, a wound healing agent, or an anti-inflammatory agent. The use of a hexapeptide of formula (I) specified in claim 1 or a pharmaceutically acceptable salt of said hexapeptide, or a pharmaceutical preparation thereof, in the manufacture of a medicament to cause an anti-edematous, anti-shock, anti-thrombus, anti-arteriosclerotic, anti-allergic, wound healing or anti-inflammatory effect in a patient. The use of a hexapeptide of formula (II) specified in claim 2 or a pharmaceutically acceptable salt of said hexapeptide, or a pharmaceutical preparation thereof, in the manufacture of a medicament to cause an anti-edematous, anti-shock, anti-thrombus, anti-arteriosclerotic, anti-allergic, wound healing or anti-inflammatory effect in a patient.

Inventors: ABURADA MASAKI, AMAGAYA SAKAE, FUJIWARA TADAMI, NAGASAWA MICHIO, OYAMA TSUTOMU, ABURADA, MASAKI, AMAGAYA, SAKAE, FUJIWARA, TADAMI, NAGASAWA, MICHIO, OYAMA, TSUTOMU, Aburada, Masaki, c/o Tsumura &amp; Co., Amagaya, Sakae,c/o Tsumura &amp; Co.,, Fujiwara, Tadami, c/o Tsumura &amp; Co., Nagasawa, Michio, c/o Tsumura &amp; Co., Oyama, Tsutomu,c/o Tsumura &amp; Co.
Applicants: TSUMURA & CO, TSUMURA & CO.
Application year: 1992
Main classification: C07K7
IPC codes: A61P3, A61P43, A61P29, A61P9, A61K38, A61P37, C07K5, C07K7, A61P7