Metal complexes of acid adducts to dioxime ligands useful in labelling proteins and other amine-containing compounds

Abstract: Boronic acid adducts of metal dioxime complexes are useful as reagents for labeling proteins and other amine-containing compounds.

Description: The invention concerns metal complexes of boronic acid adducts to dioxime ligands, useful as protein and amine labeling agents. Documents EP-A-199 260 and 311 891 disclose compounds of formula XM[R 1 C(NOH)-CR 2 (NOH)] 3 B-R 3 in which X is an anion; M is a labeling metal, namely Tc or Re; R 1 and R 2 are H, halogens, aliphatic, aromatic, or heterocyclic substituents; B is boron; and R 3 can be HO-, alkyl-, alkenyl-, cycloalkyl-, cycloalkenyl-, alkoxy-, HOOC-alkyl-, HOOC-alkenyl-, HO-alkyl-, HO-alkenyl-, alkoxyalkyl-, alkoxyalkenyl-, haloalkyl-, haloalkenyl-, aryl-, aryl-alkyl-, R 4 R 5 N-alkyl-, with R 4 and R 5 being H or alkyl, or forming together 5- or 6-membered heterocycles. Document EP-A-441 491 cited under Art 54(3-4) EPC discloses compounds of formula XM[R 1 C(NOH)-CR 2 (NOH)] 3 B-(A 1 ) p -R 3 in which X, M, R 1 and R 2 are defined as in the foregoing documents, while R 3 is or contains a biochemically active group (page 3, line 27) selected from hypoxia mediated nitro-heterocycles; amphetamines; steroids, e.g. estrogen or estradiol; sugars; fatty acids; barbiturates; sulfonamides; monoamine oxidase substrates and inhibitors; antihypertensives; substrates for muscarine receptors; and substrates for dopamine receptors. Biochemically active groups are further defined in page 4, line 24 of the citation as "molecules having affinity for a specific receptor". A 1 is a bond or a spacer group (p being an integer from 0 to about 100) that can form a linear or branched chain, and the A 1 's, are selected from -CHR 4 -, -CR 4 R 5 -, -CH=CH-, -CH=CR 4 -, -CR 4 =CR 5 -, -C≡C-, cycloalkylene, cycloalkenylene, arylene, heterocyclo, -O-, -S-, -CO-, -CS-, -NH-, -CH=N-, -CR 4 =N-, -NR 4 -, alkylene, oxaalkylene, hydroxyalkylene, hydroxyalkoxyalkylene, alkenylene, arylalkylene, alkylamido, arylalkylamido, alkylaminoalkylene, -(CH 2 ) 1-3 -, -CH 2 -CH=CH-CH 2 -, -(CH 2 ) 1-2 -CO-NH-(CH 2 ) 1-3 -, -Ph-(CH 2 ) 1-2 -, -CH 2 ) 2 O-, -CH 2 CH(OH)CH 2 OCH 2 -, -CH 2 -CO-NH-CH 2 -Ph-, -(A 3 -O-A 3 ')- and -(A 3 -NH-A 3 ')- in which A 3 and A 3 , are the same or different alkyl and R 4 and R 5 are independently selected from alkyl, alkenyl alkoxy, aryl, 5- or 6-membered N and O-containaing heterocycles, halogen, -OH, or alkyl-OH. The metal complexes of boronic acid adducts to dioxime ligands of the present invention have formula II XM[R 1 C(NOH)-CR 2 (NOH)] 3 B-(A 1 ) p -E or a pharmaceutically acceptable salt thereof, wherein: M is an isotope of technetium or rhenium; X is an anion, and R 1 and R 2 are each independently H, halogen, alkyl, aryl, or a 5- or 6-membered N-, S-, or O-containing heterocycle, or together R 1 and R 2 are -(CR 8 R 9 ) n - where n = 3, 4, 5, or 6 and R 8 and R 9 are each independently H or alkyl; B is boron; (A 1 ) is a chemical moiety which distances or otherwise isolates the moiety represented by "E" from the rest of the complex II, p being zero or an integer up to 100, preferably 1 to 20; and E is a group of formula wherein R 3 is a function reactive toward amines and proteins selected from -NCS, -NCO, -O-Tosyl, -O-Mesityl or derivatized acyl halides, activated esters, and N-hydroxysuccinimide; and R 4 is H or a water solubilizing function selected from -OH, -COOH, -SO 3 H, alkyl sulfonates, -SO 2 NH 2 , -Alk-COOH, -Alk-OH. The isothiocyanate group -N=C=S is preferred. R 4 may be ortho, meta or para to R 3 . It is preferred that R 4 is in a meta position in relationship to both R 3 and the spacer group (A 1 ) p . (A 1 ) in the boron derivatives of formula II can be any chemical moiety which can serve to distance or otherwise isolate the moiety represented by "E" from the rest of the complex of formula II. For example, wherein p is an integer greater than zero, A 1 , or the various A 1 units that form a straight or branched chain are independently selected from alkylene, alkenylene, cycloalkylene, cycloalkenylene, oxaalkylene, hydroxyalkylene, carboxyalkylene, alkylene-amido, alkylenesulfonato, arylene, arylalkylene, heterocyclo, -CH 2 -, -CHR 6 -, -CR 6 R 10 -, -CH=CH-, -CH=CR 11 -, -CR 6 =CR 12 -, -C≡C-; -O-, -S-, -CO-, -HC=N-, -CR 6 =N-, or -NR 6 -, in which R 6 , R 10 , R 11 and R 12 are independently selected from alkyl, alkenyl, alkoxy, aryl, 5- or 6-membered N-containing heterocyclic rests, halogen, -OH, or hydroxyalkyl. In the complexes of the present invention, the preferred moieties for -(A 1 ) p - are alkylene, arylene, oxaalkylene, hydroxyalkylene, hydroxyalkyleneoxy, carboxyalkylene, arylalkylene, arylalkylamido, alkylamido and alkylsulfonato. The most preferred boron radicals are the compounds of formula wherein p is zero or one, and R 4 is hydrogen or a water solubilizing functionality such as -COOH, carboxylalkyl, hydroxyalkyl, -SO 3 H, alkyl sulfonate, sulfonamide or hydroxyl. The term anion refers to any chemical entity which bears a negative charge. Exemplary groups are Cl - , Br - , F - , I - , HO - , RS - , S=C=N - and N≡C-S - . 30 The preferred anionic moieties are Cl - and HO - . Listed below are definitions of the terms used to describe the complexes of this invention. These definitions apply to the terms as they are used throughout the specification (unless they are otherwise limited in specific instances) either individually or as part of a larger group. The term "alkyl" refer to both straight and branched chain groups. Groups having 1 to 10 carbon atoms are preferred. The term "aryl" refers to phenyl and substituted phenyl. Preferred are phenyl and phenyl substituted with 1, 2 or 3 alkyl, haloalkyl, dialkylaminoalkyl, alkoxy, alkoxyalkyl, halogen, hydroxy, or formyl groups. The expression "5 or 6-membered nitrogen containing heterocycle" refers to all 5- and 6-membered rings containing at least one nitrogen atom. Exemplary aliphatic groups are dehydro derivatives of a compound having the formula wherein m is 0 or 1 and A is oxygen, sulfur, N-R 13 or CH-R 14 , wherein R 13 is alkyl, aryl, or arylalkyl and R 14 is hydrogen, alkyl, aryl or arylalkyl. Also included within the expression "5- or 6-membered nitrogen containing heterocycle" are aromatic groups. Exemplary heteroaromatic groups are pyrrolyl, imidazolyl, oxazolyl, pyrazolyl, pyridinyl, and pyrimidinyl groups. The above groups can be linked via a hetero atom or a carbon 5 atom. The expression "5- or 6-membered nitrogen or oxygen containing heterocycle" refers to all 5- and 6-membered rings containing at least one nitrogen, sulfur or oxygen atom. Exemplary groups are those described above under the definition of the expression "5 or 6-membered nitrogen containing heterocycle". Additional exemplary groups are 1,4-dioxanyl, furanyl and thiophenyl. It should be understood that the various moieties in (A1)p, or R 1 and R 2 of the vicinal dioxime should not contain functional groups that will react with the R 3 group on the boron derivatives of formula IV. Preparation of the technetium complexes of this invention can best be accomplished using technetium-99m in the form of the pertechnetate ion. The pertechnetate ion can be obtained from commercially available technetium-99m parentdaughter generators; such technetium is in the +7 oxidation state. The generation of the pertechnetate ion using this type of generator is well known in the art, and is described in more detail in documents US-A-3,369,121 and 3,920,995. These generators are usually eluted with saline solution and the pertechnetate ion is obtained as the sodium salt. Preparation of the complexes of this invention wherein M is an isotope of rhenium can best be accomplished using a source of rhenium in the +3, +4, +5 or +7 oxidation state. Examples of compounds in which rhenium is available in the + 3 oxidation state are ReCl 3 (CH 3 CN)(PPh 3 ) 2 and [Re 2 Cl 8 ] (NBu 4 ) 2 wherein Ph = phenyl and Bu = butyl. Re (IV) is available as K 2 ReCl 6 and Re (VII) is available as NH 4 ReO 4 or KReO 4 . Re (V) is available as [ReOCl 4 ](NBu 4 ) and [ReOCl 4 ](AsPh 4 ) and as ReOCl 3 (PPh 3 ) 2 and as ReO 2 (pyridine) 4 + . Other Re (III) , Re (IV) , Re (V) , Re (VII) reagents known to those skilled in the art can also be used. To prepare the complexes of this invention requires a source of the metal ion as described above which is combined with a source of anion, a boronic acid derivative having the formula VII (OH) 2 B(A 1 ) p -E and a dioxime of formula HO-N=CR 1 -CR 2 =N-OH or a pharmaceutically acceptable salt thereof. Alternatively, the complexes of this invention can be prepared by the reaction of a boronic acid derivative of formula VII with a compound of the formula XM[R 1 C(NOH)-CR 2 (NOH)]3. U.S. 4,714,605 discloses a compound MX(dioxime) 3 wherein M is technetium. Complexes of the formula MX(dioxime) 3 wherein M is an isotope of rhenium can be prepared by combining a source of Re (III) such as ReCl 3 (CH 3 CN)(PPh 3 ) 2 or [Re 2 Cl 8 ](NBu 4 ) 2 with a dioxime of formula HO-N=CR 1 -CR 2 =N-OH and a source of anion X. The formation of complexes ReX(dioxime) 3 proceeds when the mixture of Re (III) starting material, source of anion, and dioxime ligand are heated to 25 - 100°C for about 2 to 30 minutes at a pH of about one to about 4. If the complexes of formula II are prepared from pertechnetate or perrhenate, the source of the anion moiety can be water or any compound or salt that dissociates to release an appropriate anion. Exemplary compounds include NaCl, NaBr and thiol containing compounds. The formation of the complexes of formula II proceeds when the mixture of metal ion, source of anion, boronic acid derivative and dioxime is heated at about 25°C to about 100°C for about two minutes to about 30 minutes. The preferred temperature is about 50°C to about 100°C and the preferred time of heating is from about two minutes to about 15 minutes. The optimal pH for the above reaction is from about 1 to about 4. If the source of technetium or rhenium is pertechnetate or perrhenate, or other starting material in an oxidation state higher than +3, the reaction mixture should also contain a reducing agent. Stannous ions are the preferred reducing agent, and can be introduced in the form of a stannous salt such as a stannous halide (e.g. stannous chloride or stannous fluoride). The reducing agent should be present in a concentration of about 1.5 micromolar to 6.6 millimolar. Various complexing agents (also known in the art as chelating agents) can be included as part of the complexing reaction. The complexing agent should, of course, be pharmaceutically acceptable. Exemplary complexing agents are diethylenetriaminepentaacetic acid (DTPA), ethylene glycol-bis(βaminoethylether)-N,N,N',N'-tetraacetic acid (EGTA), ethylenediamine-tetraacetic acid (EDTA), citric acid, tartaric acid, malonic acid, etc. The complexing reaction mixture can also include an accelerator (catalyst) which serves to improve the radiochemical purity (i.e., the % of the radioactivity that is in the desired chemical form) of the product. Exemplary accelerators are the α-hydroxycarboxylic acids such as citric acid, tartaric acid, and malonic acid. A combination of DTPA and citric acid has been found to be preferred. As it is necessary to prepare the complexes of this invention at, or near, the site where they are to be used, a kit having all of the components, other than the source of the technetium or rhenium, to prepare the boronic adducts of the metal dioxime complexes of formula I is an integral part of this invention. Such a kit contains a source of anion, a boronic acid derivative of formula VIII (or compounds which can react in situ to form such a derivative), or a pharmaceutically acceptable salt thereof, a dioxime of formula HO-N=CR 1 -CR 2 =N-OH, or a pharmaceutically acceptable salt thereof, and a reducing agent. It may optionally contain complexing agent(s), accelerator(s), solubilization agents, and/or other components necessary to provide high radiochemical purity. A multi-vial kit may also be used to prepare the complexes of this invention. For example, one vial of the kit could contain a source of anion, a dioxime ligand of formula HO-N=CR 1 -CR 2 =N-OH or pharmacologically acceptable salt thereof, a reducing agent, and optional complexing agents. A source of technetium or rhenium is added to this kit, which is then heated to form the intermediate MX(dioxime) 3 of formula XM[R 1 C(NOH)-CR 2 (NOH)] 3 (M = Tc,Re). The intermediate product from this kit is then added to a second vial that contains a boronic acid derivative of formula VIII (or a compound which can react in situ to form such a derivative). The kits of this invention can be formulated in aqueous solution. To optimize the stability of the kit, and to optimize the radiochemical purity of the labeled product, the pH of the kit should be adjusted to fall within the range of about 1.0 to 4.0 using a pharmaceutically acceptable acid or base (e.g., hydrochloric acid or sodium hydroxide). Preferably, the pH of the kit will be about 2.0. It is also preferred that the kit be in lyophilized form. While "wet" kits (i.e. kits which provide all components in solution) can be used, they are not as efficacious as the corresponding lyophilized kit. The compounds of formula VIII wherein R 3 is NCS are novel and form an integral part of this invention. These boronic acids of formula VIII or pharmaceutically acceptable salts thereof are bifunctional reagents that will react with the above kit constituents to form the complexes of formula II. The reactive group R 3 will react with proteins or with other amine-containing compounds either before or after reaction with the metal M. Working with the 99 Technetium isotope, the structure of one of the complexes of this invention has been investigated and is believed to be Coupling of the metal complexes of formula II, to proteins such as monoclonal and polyclonal antibodies, polypeptides such as albumin, insulin, hemoglobin and fibrinogen, hormones such as angiotensin, adrenalin, and amine containing steroid, derivatives, amino acids such as glycine and cysteine, drugs such as penicillamine and alkaloids, or other amine-containing compounds such as enzymes and whole cells occurs via reaction of the group R 3 on the boron derivative with primary or secondary amines on the protein or other aminecontaining compounds. If R 3 is isothiocyanato (-N=C=S), the resultant thiourea bond is chemically very stable. Thus, a stable covalent link is formed that attaches the metal dioxime complexes of formula II to the protein or other amine-containing compound. An example of this reaction is shown below. wherein DMG = dimethylglyoxime. It is also possible to form complexes where the group E on the boron moiety has not initially a reactive functionality. This complex could then be converted, after the formation of the complex core, to another more reactive compound II that contains a group E that is reactive. For example, compounds that contain a boron moiety such as B-Ph-CH 2 OH can be converterted after formation into active compounds, e.g. B-Ph-CH 2 OTs (Ts = tosyl). This activated compound could then be used for reaction with proteins. Radiolabelled antibodies and antibody fragments are envisioned by this invention and are useful either as diagnostics or in radiotherapy. Antibody fragments include Fab, F(ab') 2 , and Fab'. They are described by S.W. Burchiel et al. "Immunopharmokinetics of Radiolabeled Antibodies and their Fragments", Chapter 13 in "Tumor Imaging, The Radiochemical Detection of Cancer", editors S.W. Burchiel and B.A. Rhodes, Masson Publishing Inc., (1982). Uses of these radiolabeled antibodies and antibody fragments include the detection of clots and the diagnosis and treatment of cancer. Substrates such as drugs, proteins or hormones that are radiolabeled using the method described herein may be used to provide diagnostic anatomic images of organ systems, images of organ function, probes to biochemical processes and the diagnosis and treatment of cancer. As described in further detail in the example section, it has been demonstrated that certain antibodies and antibody fragments labelled in the above-described manner retain their biological activity and hence are capable of localizing preferentially in tumors that express antigens that are recognized by a specific antibody. Because of this preferential localization of radiolabeled materials in tumors, antibodies and antibody fragments labelled in the manner described in this invention are useful in the diagnosis or therapy of cancer. The antibody-boronic acid-metal dioxime complex conjugate is relatively non-reactive with tissue not associated with the tumor or cancer. Either the complexes of this invention or the proteins or antibodies labeled with the complexes of this invention can be administered to a host by bolus intravenous injection or intraperitoneally. The size of the host, and the imaging system used, will determine the quantity of radioactivity needed to produce diagnostic images. For a human host, the quantity of radioactivity injected will normally range from about 5 to 20 millicuries of technetium-99m. The above mode of administration is also applicable when the complexes of this invention, by themselves, or when coupled to antibodies or proteins are used in radiotherapy. The quantity of radioactivity in radiotherapy for a human host will range from about 10 to 30 millicuries. The following examples are specific embodiments of this invention. Example 1 m-Isothiocyanatophenylboronic Acid To a cooled (0°C) solution of 3-aminophenyl-boronic acid (2.24 g, 0.013 mol) in acetone (5 ml) was added dropwise a solution of thiophosgene (1 ml, 0.013 mol) in chloroform (5 ml) over a period of 0.5 hour. The reaction mixture was stirred at 0°C for 1 hour and at room temperature for 12 hours. The solvent was evaporated on a rotary evaporator and the residue was triturated with ether and filtered to remove the triethylamine hydrochloride. The ether solution was evaporated to a brown oil. This was chromatographed on silica gel (30 g) using acetone-chloroform (7:3) as eluent. Fractions containing the product were collected and evaporated to give a light brown solid. This solid was crystallized from ethyl acetate/hexane. M.P. 166-67°C. Anal. Calcd. for C 7 H 6 NSBO 2 .0.25H 2 O: C, 46.00; H,3.59; N, 7.67; S, 17.54. Found: C, 46.17; H, 3.48; N, 7.59; S,17.71. Example 2 3-Isothiocyanato-5-carboxyphenylboronic Acid Example 2A m-Tolylboronic Acid A solution of m-bromotoluene (25 g. 0.146 mol) in ether (200 ml) was added dropwise to magnesium (4.0 g, 0.17 mol) in ether (50 ml) at room temperature over a period of 3 hours under a nitrogen atmosphere. After the addition, the reaction mixture was stirred for 12 hours at room temperature. The Grignard reagent was added slowly to a solution of trimethyl borate (15.6 g, 17 ml, 0.15 mol) in ether (500 ml) over a period of 4 hours at -78°C and stirred for 12 hours at room temperature. The reaction mixture was hydrolyzed with 30% sulfuric acid (50 ml). The ether layer was separated, washed with water (3 x 250 ml) and dried over Na 2 SO 4 . The solvent was removed on a rotary evaporator and the solid obtained was recrystallized from water. M.P. 160-161°C. Example 2B m-Carboxyphenylboronic Acid To a solution of m-Tolylboronic acid (8.0 g, 0.059 mol) in 10% sodium hydroxide (250 ml) was added potassium permanganate (20 g) in small portions. The reaction mixture was stirred at room temperature for 48 hours. The precipitated manganese dioxide was filtered and the filtrate was acidified with 10% sulfuric acid and cooled. The white precipitate that formed was filtered and recrystallised from water. M.P. 250-52°C. Example 2C 3-Nitro-5-carboxyphenylboronic Acid To a stirred slurry of m-carboxyphenylboronic acid (5 g, 0.03 mol) in concentrated sulfuric acid (15 ml) was added fuming nitric acid (15 ml, d = 1.54). The reaction mixture was stirred at room temperature for 45 minutes and poured on ice (150 g). The nitro acid formed was filtered, washed with water and dried. M.P. 235-37°C. Example 2D 3-Amino-5-carboxyphenylboronic Acid A solution of 3-nitro-5-carboxyphenylboronic acid (3.3 g, 0.016 mol) in absolute ethanol (25 ml) was hydrogenated in the presence of Raney Nickel (1 g) at 50 lbs per square inch for 4 hours in a Parr shaker. The catalyst was removed by filtration and the solvent was removed on a rotary evaporator. The solid obtained was recrystallized from water. M.P. 210-12°C. Example 2E 3-Isothiocyanato-5-carboxyphenylboronic Acid To a solution of 3-amino-5-carboxyphenylboronic acid (150 mg, 0.08 mmol) in 3N HCl (3 ml) was added thiophosgene (92 mg, 0.08 mmol) in 3N HCl (3 ml) and the reaction mixture was stirred at room temperature. A white solid was formed after 20 minutes. This was recrystallized from hexane/ethyl acetate. M.P. >350°C. Anal. Calcd. for C 8 H 6 NO 4 BS.3H 2 O. C, 42.06; H, 2.91; N, 6.13. Found: C, 42.51; H, 2.87; N, 5.68. Example 3 99m Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanatophenyl) boron To a freeze-dried mixture of 2.0 mg of dimethyl glyoxime, 100 mg of sodium chloride, 18 mg of citric acid, 1 mg of diethylenetriaminepenta-acetic acid (DTPA), 50 mg of gammacyclodextrin, and 50µg of stannous chloride in a 5 ml siliconized serum vial was added 3.0 mg of 3-isothiocyanatophenylboronic acid in 50 µl of ethanol. Sodium pertechnetate in physiological saline (1.0 ml) was added to the vial, which was then heated at 100°C for 5 minutes. The yield of Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanatophenyl) boron, as determined by high pressure liquid chromatography (HPLC), was 35-40%. Samples of this complex coelute from Lichrosorb HPLC columns with authentic samples of 99 Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanatophenyl) boron that was prepared as described below. Example 3a 99 Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanatophenyl) boron A solution containing 99 Tc(dimethyl glyoxime) 3 (µ-OH)(SnCl 3 )·3H 2 O (97 mg, 0.138 mmol), 3-isothiocyanatophenylboronic acid (32 mg, 0.18 mmol), ethanol (10 ml), and 1M HCl (2 ml) was boiled gently in a small beaker for 1 hour in air. The resulting red-orange precipitate (60% yield) was recrystallized from a warm CH 2 Cl 2 /EtOH mixture (1:5). X-Ray structure quality crystals were formed within one day. Anal. (Calcd.) for C 19 H 24 N 7 O 6 BClSTc: C, 36.36 (36.59); H, 3.80 (3.89); N, 15.88 (15.72). Molecular ion MH + observed by mass spectroscopy at m/z=624. Retention time = 8.4 minutes on Licrosorb HPLC column (75/25 ACN/0.1M NH 4 OAc pH 4.6, 1.5 mL/min.) An X-Ray crystal structure determination on this complex confirmed the structure. Example 4 99m Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron Following the procedure for Example 4, but substituting 3.0 mg of 3-isothiocyanato-5-carboxyphenyl boronic acid for the 3-isothiocyanatophenylboronic acid, yielded 35 - 40% of the title complex. Samples of this complex coelute from a Nucleosil HPLC column with authentic samples of 99 Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanato-5-carboxyphenyl) boron prepared as described in 5a below. Example 4a 99 Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron To the complex Tc(chlorine)(dimethyl glyoxime) 3 [TcCl(DMG 3 )] (23.8 mg, 0.049 mmol) in 3 ml of CH 3 CN was added 3 drops of 1M HCl and 11.5 mg (0.051 mmol) of 3-isothiocyanato-5-carboxyphenyl boronic acid. The reaction mixture was heated at 50°C with stirring for 15 minutes. It was then treated with an equal volume of 1M HCl and cooled to room temperature. The resulting red-orange precipitate was recrystallized from CH 3 CN/1M HCl to yield analytically pure needles (75.5% yield), isolated as the 1.5 H 2 O hydrate. Anal. (Calcd.) for C 20 H 24 N 7 BClO 8 STc·1.5H 2 O: C, 34.76 (34.57); H, 3.90 (3.92); N, 13.90 (14.11). Retention time = 2.85 min., Nucleosil HPLC column (65/35 ACN/0.1M citric acid, 1.5 ml/min.). Example 5 99m Tc(chlorine)(1,2-cyclohexanedione dioxime) 3 (3-isothiocyanatophenyl)boron To a freeze-dried mixture of 1.5 mg of 1,2-cyclohexanedionedioxime, 100 mg of sodium chloride, 9 mg of citric acid, 2 mg of diethylenetriamine-penta-acetic acid (DTPA), 50 mg of gamma-cyclodextrin, and 50 µg of stannous chloride in a 5 ml siliconized serum vial was added 2.4 mg of 3-isothiocyanatophenylboronic acid in 50 µl of ethanol. Sodium pertechnetate in physiological saline (1.0 ml) was added to the vial, which was then heated at 100°C for 15 minutes. The yield of Tc(chlorine) (1,2 cyclohexanedione dioxime) 3 (3-isothiocyanatophenyl) boron, as determined by high pressure liquid chromatography (HPLC), was 33%. Samples of this complex coelute from Licrosorb HPLC columns with authentic samples of a 99 Tc standard, prepared as described in 6a below. Example 5a 99 Tc(chlorine)(1,2-cyclohexanedione dioxime) 3 (3-isothiocyanatophenyl)boron A solution containing 99 Tc(1,2-cyclohexanedione dioxime) 3 (µ-OH)SnCl 3 ·3H 2 O (77 mg, 0.095 mmol), 3-isothiocyanatophenylboronic acid (22 mg, 0.12 mmol), ethanol (10 ml), and 1M HCl (2 ml) was boiled in a small beaker for 1 hour in air. During this time, the volume of the solution reduced to 2 ml, and orange crystals of product precipitated (66% yield). The product was purified on a silica gel column; elution with 5% ACN/95% CH 2 Cl 2 yielded analytically pure product upon evaporation of solvent. Anal. (Calcd.) for C 25 H 30 N 7 BClO 6 STc: C, 42.65 (42.78); H, 4.44 (4.31); N, 14.26 (13.87). Samples of this complex eluted from Licrosorb HPLC columns at a retention time of 8 minutes (90/10 ACN/0.1M NH 4 OAc pH 4.6, 1.5 ml/min.). Example 6 99m Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron Following the procedure of Example 6, but substituting 3.0 mg of 3-isothiocyanato-5-carboxyphenylboronic acid for the 3-isothiocyanatophenylboronic acid, yielded 35% of the title complex. Samples of this complex eluted from Nucleosil HPLC columns at a retention time identical to that of a 99 Tc standard of the title complex, prepared as described in Example 12a. Example 7 99m Tc(chlorine)(dimethyl glyoxime) 3 Following the procedure of example 4, but omitting the boronic acid entirely, and heating the kit for 2.5 minutes at 100°C, the complex 99m TC(chlorine) (dimethyl glyoxime) 3 was prepared in 76% yield. Example 8 99m Tc(chlorine) (dimethyl glyoxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron To 99m Tc(chlorine) (dimethyl glyoxime) 3 prepared as described in Example 8, was added 3.0 mg of 3-isothiocyanato-5-carboxyphenylboronic acid in 50 µl of ethanol. The mixture was heated at 100°C for 5 minutes yielding the title compound in 62% yield. Samples of this complex coeluted from Nucleosil HPLC columns at a retention time identical to that of an authentic 99 Tc standard of the complex, prepared as described in Example 5a. Example 9 99m Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 Following the procedure of Example 6, but omitting the boronic acid entirely, the yield of 99m Tc(chlorine)(1,2-cyclohexanedionedioxime) 3 was 92%. Example 10 99m Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 (3-isothiocyanatophenyl)boron To 99m Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 prepared as described in Example 10, was added 2.4 mg of 3-isothiocyanatophenylboronic acid in 50 µl of ethanol. The mixture was heated for 15 minutes at 100°C. The yield of title complex, prepared by this route, was 48.5%. Samples of this complex coeluted from Licrosorb HPLC columns at a retention time identical to that of an authentic 99 Tc standard that was prepared as described in Example 6a. Example 11 99m Tc(chlorine)(1,2-cyclohexanedione dioxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron A sample of 99m Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 was prepared as described in Example 10. The complex was isolated, and kit components were removed, by purification on Hamilton PRP-1 resin, as follows. The kit contents were drawn up into a needle-hub containing the resin and then rinsed with 1 ml of saline, 1 ml of 25/75 EtOH/saline, 1 ml of H 2 O, and 1 ml of EtOH. An 0.5 ml aliquot of the ethanol fraction, which contained purified 99m Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 was added to a vial that contained 1.5 mg of 3-isothiocyanato-5-carboxyphenyl)boron and 50 µl of 1N HCl. This kit was heated for 5 minutes at 100°C. This procedure gave the title complex in 81.7% yield. Samples of this complex co-eluted from HPLC columns at the retention time of an authentic 99 Tc standard of the complex that was prepared as described in 12a below. Example 11a 99 Tc(chlorine)(1,2-cyclohexanedione dioxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron To 4.0 mg of 99 Tc(chlorine) (1,2-cyclohexanedione dioxime) 3 , dissolved in 3 ml of ACN was added 1.5 mg of 3-isothiocyanato-5-carboxyphenyl boronic acid and 3 drops of 3M HCl. The solution was heated gently for 20 minutes. The complex 99 Tc(chlorine)(1,2-cyclohexanedione) 3 (3-isothiocyanato-5-carboxyphenyl)boron was formed in quantitative yield. Samples of this complex gave a strong molecular ion peak MH + by fast atom bombardment mass spectroscopy and had a retention time of 8.0 minutes on Nucleosil HPLC columns (ACN/0.1M citric acid 60/40, 1.5 ml/min.). Example 12 Labeling of glycine with 99m Tc(chlorine)(dimethyl glyoxime) 3 (3-isothiocyanatophenyl)boron (Tc-DMG-BPITC) A solution of 99m Tc(chlorine) (dimethyl glyoxime) 3 (3-isothiocyanatophenyl)boron (Tc-DMG-BPITC) prepared as described in Example 4, was purified by adsorption onto Reverse Phase resin (as in Example 12). After washing the resin with 50% ethanol/50% normal saline to remove impurities and excess boronic acid, the desired complex was eluted from the resin with ethanol. A 200 µl aliquot of this ethanol solution was mixed with 200 µl of glycine (20 mg/mL in 0.1 M sodium phosphate buffer pH 9.0). The mixture was incubated at 37°C for 1 hour. Analysis of the reaction by HPLC showed that all starting material had disappeared at this time. The yield of Tc-labeled glycine (Tc-DMG-BPITC-Glycine) was 88%, as determined by HPLC. Example 13 Labeling of polylysine with 99m Tc-DMG-BPITC A sample of Tc(chlorine) (dimethyl glyoxime) 3 (3-isothiocyanatophenyl)boron(Tc-DMG-BPITC), prepared as described in Example 4, and purified as described in Example 14, was evaporated to dryness with a nitrogen stream. The complex was re-dissolved in 50 µl of DMSO, and 10 µl of this DMSO solution was added to 100 µl of pH 9.0 0.1M sodium phosphate buffer that contained 0.5 mg of polylysine (average m.w. = 102,000). After incubation at 37°C for 2 hours, the yield of Tc-labelled polylysine (Tc-DMG-BPITC-polylysine) was 36%, as estimated by HPLC on a Pinkerton ISRP column. Example 14 Labeling of mouse IgG with 99m Tc-DMG-BPITC A sample of Tc(chlorine) (dimethyl glyoxime) 3 -(3-isothiocyanatophenyl)boron (Tc-DMG-BPITC), prepared as described in Example 4, and purified as described in Example 14, was evaporated to dryness with a nitrogen stream. The complex was re-dissolved in 50 µl of DMSO, and 10 µl of this DMSO solution was added to 100 µl of pH 9.5 0.1M sodium phosphate buffer that contained 0.5 mg of mouse IgG. After incubation at 37°C for 2.5 hours, the yield of Tc-labeled mouse IgG was 34%, as estimated by HPLC on a Pinkerton ISRP column. Example 15 Labeling of the Monoclonal Antibody B72.3 With 99m Tc-DMG-BPITC A sample of Tc(chlorine) (dimethyl glyoxime) 3 (3-isothiocyanatophenyl)boron (Tc-DMG-BPITC), prepared as described in Example 4, and purified as described in Example 14, was evaporated to dryness with a nitrogen stream. The complex was re-dissolved in 50 µl of DMSO, and 10 µl of this DMSO solution was added to 100 µl of pH 9.5 0.1M sodium phosphate buffer that contained 1.0 mg of antibody B72.3. This antibody is described by D. Colcher et al., Nucl. Med. Biol., Vol. 14 (3) p. 251 - 262 (1987). After incubation at 37°C for 2.0 hours, the yield of Tc-labeled B72.3 was 10%, as estimated by HPLC on an ISRP-TSK column. Example 16 Labeling of the Monoclonal Antibody B72.3 with 99m Tc-DMG-NCS-COOH . A sample of Tc(chlorine) (dimethyl glyoxime) 3 (3-carboxy-5-isothiocyanatophenyl)boron (Tc-DMG-NCS-COOH), prepared as described in Example 5, and purified as described in Example 14, was evaporated to dryness with a nitrogen stream. The complex was re-dissolved in 100 µl of pH 9.5 0.1M sodium phosphate buffer that contained 1.0 mg of B72.3. After incubation at 37°C for 2.0 hours, the yield of Tc-labeled B72.3 was 34%, as estimated by HPLC on an ISRP-TSK column. Example 17 Affinity Chromatography of the Monoclonal Antibody B72.3 Labelled with 99m Tc-DMG-NCS-COOH A sample of 99m Technetium labelled B72.3, prepared as described in Example 18, was purified from excess labelling reagent by passage through an ISRP and TSK column, linked in series. An aliquot of the purified antibody (about 50,000 cpm/128 ng of protein) was loaded onto a TAG-72 affinity column and incubated at room temperature. The column was washed with PBS buffer to elute non-specifically bound protein. The active labelled antibody was then eluted from the column with 6M guanidine. The immunoreactivity of the antibody, expressed as a percentage, is defined as [ (activity in cpm eluted with guanidine/total activity in cpm eluted from the column)x100 ]. An immunoreactivity of 72% was seen. For comparison, a sample of B72.3 labeled by standard methods with 125 Iodine was 55-60% immunoreactive, as assayed using a TAG-72 affinity column. Example 18 Biodistribution of 99m Tc-labeled B72.3 in Tumor-bearing mice Tumor-bearing (GW39 tumor line) nude mice were injected with 10-20 uCi of Tc-labelled B72.3 prepared as described in Example 18 and purified by HPLC as described in Example 19. The animals were sacrificed at 2 or 24 hours (n=5 animals for each time point), and organs of interest dissected and counted for radioactivity. For comparison, 131 Iodine labelled B72.3 was injected into a control set of tumor-bearing mice. Data from this experiment (enclosed as Table 1), indicated specific localization of both the Iodine and Tc-labelled B72.3 in the tumors. Example 19 Labeling of the Monoclonal Antibody NP-4 With 99m Tc-DMG-NCS-COOH A sample of Tc(chlorine)(dimethyl glyoxime) 3 (3-carboxy-5-isothiocyanatophenyl)boron (Tc-DMG-NCS-COOH), prepared as described in Example 5, and purified as described in Example 14, was evaporated to dryness with a nitrogen stream. The complex was re-dissolved in 100 µl of pH 9.5 0.1M sodium phosphate buffer that contained 1.0 mg of NP-4 (CMMI, Newark, New Jersey). After incubation at 37°C for 2.0 hours, the yield of Tc-labeled NP-4 was 40%, as estimated by HPLC on an ISRP-TSK column. Example 20 Affinity Chromatography of the Monoclonal Antibody NP-4 Labelled With 99m Tc-DMG-NCS-COOH A sample of 99m Technetium labelled NP-4, prepared as described in Example 21, was purified from excess labelling reagent by passage through an ISRP-TSK HPLC column. An aliquot of the purified antibody (about 50,000 cpm/128 ng of protein) was loaded onto an anti-CEA affinity column and incubated at room temperature. The column was washed with PBS buffer to elute non-specifically bound protein. The active labelled antibody was then eluted from the column with 6M quanidine. The immunoreactivity of the antibody, expressed as a percentage, is defined as [ (activity in cpm eluted with guanidine/ total activity in cpm eluted from the column)x100 ]. An immunoreactivity of 95.8% was seen. Example 21 Biodistribution of 99m Tc-labeled NP-4 in Tumor-Bearing Mice Tumor-bearing (GW39 tumor line) nude mice were injected with 50µCi of Tc-labelled NP-4 prepared as described in Example 21 and purified by HPLC as described in Example 22. The animals were sacrificed at 2 or 24 hours (n=6 animals for each time point), and organs of interest dissected and counted for radioactivity. Data from this experiment (enclosed as Table 2), indicated specific localization of the 99m-Tc-labelled NP-4 in the tumors. At 24 hours, an average of 16.56% of the injected dose was seen per gram of tumor. Example 22 Labeling of the Monoclonal Antibody F(ab') 2 Fragment of NP-4 with 99m Tc-DMG-NCS-COOH A sample of Tc(chlorine)(dimethyl glyoxime) 3 (3-carboxy-5-isothiocyanatophenyl)boron (Tc-DMG-NCS-COOH), prepared as described in Example 5, and purified as described in Example 14, was evaporated to dryness with a nitrogen stream. The complex was re-dissolved in 100 µl of pH 9.5, 0.1M sodium phosphate buffer that contained 1.0 mg of the F(ab') 2 fragment of NP-4 (CMMI, Newark, New Jersey). After incubation at 37°C for 2.0 hours, the yield of Tc-labeled NP-4-F(ab') 2 was 15.2%, as estimated by HPLC on an ISRP-TSK column. Example 23 Affinity Chromatography of the Monoclonal Antibody F(ab') 2 fragment of NP-4 Labelled with 99m Tc-DMG-NCS-COOH A sample of 99m Technetium labelled NP-4-F(ab') 2 , prepared as described in Example 24, was purified from excess labelling reagent by passage through an ISRP-TSK HPLC column. An aliquot of the purified antibody (about 30,000 cpm/100 ng of protein) was loaded onto an anti-CEA affinity column and incubated at room temperature. The column was then washed with PBS buffer to elute non-specifically bound protein. The active labelled antibody fragment was then eluted from the column with 6M guanidine. The immunoreactivity of the antibody, expressed as a percentage, is defined as [ (activity in cpm eluted with guanidine/total activity in cpm eluted from the column)x100 ]. An average immunoreactivity of 94% was seen. Example 24 Biodistribution of 99m Tc-labelled NP-4 F(ab') 2 Fragment in Tumor-Bearing Mice Tumor-bearing (GW39 tumor line) nude mice were injected with 20-30 µCi of Tc-labelled F(ab') 2 fragment of NP-4 prepared as described in Example 24 and purified by HPLC as described in Example 25. The animals were sacrificed at 2 or 24 hours (n=6 animals for each time point), and organs of interest dissected and counted for radioactivity. Data from this experiment (enclosed as Table 3), indicated specific localization of the 99m-Tc-labelled NP-4 F(ab') 2 fragment in the tumors. At 24 hours, an average of 10.5% of the injected dose was seen per gram of tumor. Example 25 Labeling of the Monoclonal Antibody B72.3 With Tc(chlorine)(1,2-cyclohexanedione dioxime) 3 (3-isothiocyanato-5-carboxyphenyl)boron A sample of 99m Tc(chlorine)(1,2-cyclohexanedione dioxime) 3 (3-isothiocyanato-5-carboxyphenyl) boron was prepared as described in Example 12, diluted to 2 ml with normal saline, purified as described in Example 14, and evaporated to dryness with a nitrogen stream. The complex was redissolved in 100 µl of pH 9.5 0.1M sodium phosphate buffer that contained 1.0 mg of the monoclonal antibody B72.3. After incubation at 37°C for 2 hours, the yield of 99m Tc-labeled B72.3 was 7.4%, as estimated by HPLC on an ISRP-TSK column. Comparison of the biodistribution of 99m Tc and 131 I labeled B72.3 in Tumor (GW39) bearing nude mice. a. 99m Tc-B72.3 (from example 16) Tissue % injected dose/gram tissue 2 hr p.i. 24 hr p.i. GW39 2.48 ± 0.749 10.47 ± 3.788 Liver 9.05 ± 0.719 6.13 ± 1.227 Spleen 7.70 ± 1.079 3.82 ± 1.098 L. Kidney 10.49 ± 3.701 5.17 ± 0.866 Lungs 14.47 ± 4.684 8.30 ± 1.984 Blood 30.66 ± 3.362 15.84 ± 2.255 Urine 7.88 ± 0.900 6.50 ± 3.640 Stomach 2.51 ± 1.950 1.60 ± 0.343 Small Int. 4.47 ± 0.522 3.31 ± 0.298 Muscle 1.37 ± 0.243 1.46 ± 0.320 Bone 2.91 ± 0.586 1.91 ± 0.414 b. 131 I-B72.3 GW39 3.10 ± 1.411 18.20 ± 8.362 Liver 7.80 ± 1.166 5.50 ± 1.129 Spleen 8.57 ± 3.169 5.11 ± 1.508 L. Kidney 11.19 ± 6.655 6.26 ± 1.027 Lungs 12.48 ± 1.577 8.46 ± 1.209 Blood 25.96 ± 7.317 18.98 ± 2.708 Urine 8.45 ± 4.887 4.28 ± 1.701 Stomach 2.46 ± 0.717 4.47 ± 1.123 Small Int. 2.04 ± 0.426 1.45 ± 0.176 Muscle 1.33 ± 0.435 2.16 ± 0.315 Bone 3.43 ± 1.212 2.63 ± 0.475 The biodistribution of 99m Tc-NP-4 whole antibody in Tumor (GW39) bearing nude mice. Tissue % injected dose/gram tissue 2 hr p.i. 24 hr p.i. GW39 1.75 ± 0.783 16.56 ± 5.822 Liver 5.79 ± 1.234 4.29 ± 0.365 Spleen 4.74 ± 0.935 3.86 ± 1.104 L. Kidney 7.14 ± 1.463 3.51 ± 0.709 Lungs 10.45 ± 1.849 5.43 ± 1.795 Blood 20.97 ± 3.594 11.46 ± 1.286 Urine 5.36 ± 0.712 7.05 ± 2.641 Stomach 1.35 ± 0.338 1.51 ± 0.458 Small Int. 1.62 ± 0.392 2.06 ± 0.409 Muscle 0.92 ± 0.240 1.37 ± 0.239 Bone 2.75 ± 0.740 1.92 ± 0.490 The biodistribution of 99m Tc-NP-4 F(ab') 2 in Tumor (GW39) bearing nude mice. Tissue % injected dose/gram tissue 2 hr p.i. 24 hr p.i. GW39 3.99 ± 0.578 10.47 ± 2.595 Liver 8.01 ± 2.383 4.16 ± 1.530 Spleen 6.22 ± 2.466 3.26 ± 2.398 L. Kidney 17.66 ± 5.709 17.95 ±10.516 Lungs 10.87 ± 3.409 3.78 ± 1.785 Blood 29.37 ± 7.068 6.24 ± 2.122 Urine 28.24 ±12.850 11.73 ± 9.797 Stomach 2.79 ± 0.545 0.71 ± 0.328 Large Int. 3.72 ± 0.543 3.15 ± 1.208 Muscle 1.30 ± 0.198 1.13 ± 0.508 Bone 4.32 ± 2.320 1.73 ± 0.829

Claims: Claims for the following Contracting States : AT, BE, CH, DE, DK, FR, GB, IT, LI, LU, NL, SE Metal complexes of boronic acid adducts to dioxime ligands, useful as protein and amine labeling agents, having formula XM[R 1 C(NOH)-CR 2 (NOH)] 3 B-(A 1 ) p -E or a pharmaceutically acceptable salt thereof, wherein: M is an isotope of technetium or rhenium; X is an anion, and R 1 and R 2 are each independently H, halogen, alkyl, aryl, or a 5- or 6-membered N-, S-, or O-containing heterocycle, or together R 1 and R 2 are -(CR 8 R 9 )n- where n = 3, 4, 5, or 6 and R 8 and R 9 are each independently H or alkyl; B is boron; (A1) is a chemical moiety which distances or otherwise isolates the moiety represented by "E" from the remainder of the complex II, p being zero or an integer up to 100, preferably 1 to 20; and E is a group of formula wherein R 3 is a function reactive toward amines and proteins selected from -NCS, -NCO, -O-Tosyl, -O-Mesityl or derivatized acyl halides, activated esters, and N-hydroxysuccinimide; and R 4 is H or a water solubilizing function selected from -OH, -COOH, -SO 3 H, alkyl sulfonates, -SO 2 NH 2 , -Alk-COOH, -Alk-OH. The complex of claim 1, in which A 1 is selected from alkylene, alkenylene, cycloalkylene, cycloalkenylene, oxaalkylene, hydroxyalkylene, carboxyalkylene, alkyleneamido, alkylenesulfonato, arylene, arylalkylene, heterocyclo, -CH 2 -, -CHR 6 -, -CR 6 R 10 -, -CH=CH-, -CH=CR 11 -, -CR 6 =CR 12 -, -C≡C; -O-, -S-, -CO-, -HC=N-, -CR 6 =N-, or -NR 6 -, in which R 6 , R 10 , R 11 and R 12 are independently selected from alkyl, alkenyl, alkoxy, aryl, 5- or 6-membered N-containing heterocyclic rest, halogen, -OH, or hydroxyalkyl. The complex of claim 1, wherein the boron carrying moiety is of formula VI in which p = 0 or 1. The complex of claims 1-3, wherein X is Cl - or OH - and the dioxime portion is dimethyl-glyoxime or a dioxime of ethane dione, 1,2-cyclopentane dione or 1,2-cyclohexane dione. A complex of claim 1, namely Tc- or Re-(chlorine)(1,2-cyclohexanedione) 3 (3-carboxy-5-isothiocyanatophenylboron); Tc- or Re-(chlorine)(1,2-cyclohexanedione) 3 (3-isothiocyanatophenylboron); Tc- or Re- (chlorine)(dimethylglyoxime) 3 (3-carboxy-5-isothiocyanatophenylboron); Tc- or Re-(chlorine)(dimethylglyoxime) 3 (3-isothiocyanatophenylboron); Tc- or Re-(chlorine)(dimethylglyoxime) 3 (4-isothiocyanatophenylboron). A method for the preparation of complexes according to claims 1-5, comprising: a) combining a Tc or Re source, a source of an anion, a boronic acid of formula VII (HO) 2 B(A 1 ) p -E, in which A 1 , p and E are defined as in claim 1, and a dioxime of formula HO-N=CR 1 -CR 2 =N-OH in which R1 and R2 are defined as in claim 1, or a pharmaceutically acceptable salt thereof; or b) reacting the foregoing boronic acid VII with a dioxime compound XM[R 1 C(NOH)-CR 2 (NOH)] 3 in which X, R1, R2 and M are as defined in claim 1. A boronic acid compound of formula VIII in which A 1 , p, R 3 and R 4 are defined as in the previous claims The boronic acid compound of claim 7, namely 3-isothiocyanatophenylboronic acid, 4-isothiocyanatophenylboronic acid or 3-carboxy-5-isothiocyanatophenylboronic acid A kit for labeling with 99m Tc or isotopic Re comprising, (i) a source of anion, e.g. Cl - ; (ii) a boronic acid compound of formula VIII in which A 1 is selected from alkylene, alkenylene, cycloalkylene, cycloalkenylene, oxaalkylene, hydroxyalkylene, carboxyalkylene, alkyleneamido, alkylenesulfonato, arylene, arylalkylene, heterocyclo, -CH 2 -, -CHR 6 -, -CR 6 R 10 -, -CH=CH-, -CH=CR 11 -, -CR 6 =CR 12 -, -C≡C-; -O-, -S-, -CO-, -HC=N-, -CR 6 =N-, or -NR 6 -, in which R 6 , R 10 , R 11 and R 12 are independently selected from alkyl, alkenyl, alkoxy, aryl, 5- or 6-membered N-containing heterocyclic rest, halogen, -OH, or hydroxyalkyl; p is 0 to 100, R 3 is a functional group selected from -NCS, -NCO, -O-Tosyl, -O-Mesityl or derivatized acyl halides, activated esters, or N-hydroxysuccinimide; and R 4 is H or a water solubilizing function selected from -OH, -COOH, -SO 3 H, alkyl-sulfonates, -SO 2 NH 2 , -Alk-COOH, -Alk-OH; (iii) a vic dioxime HO-N=CR 1 -CR 2 =N-OH or a pharmaceutically acceptable salt thereof in which R 1 and R 2 are each independently H, halogen, alkyl, aryl, or a 5- or 6-membered N-, S-, or O-containing heterocycle, or together R 1 and R 2 are -(CR 8 R 9 ) n - where n = 3, 4, 5, or 6 and R 8 and R 9 are each independently H or alkyl; and (iv) optionally, a reducing agent. A kit according to claim 7, in which the dioxime is 1,2-cyclohexanedione-dioxime or 2,3-butanedione-dioxime; and the boronic acid is 3-carboxy-5-isothiocyanatophenylboronic acid or 3-isothiocyanatophenylboronic acid. A conjugate formed by the reaction of a metal/dioxime/boron complex of claims 1-5 and amine-containing compounds, wherein said amine-containing compounds are selected from proteins, e.g. fibrin, fibrinogen and low density lipoprotein, polypeptides, hormones, amine-containing steroids, aminoacids, or amine-containing drugs and compounds. A conjugate of claim 11, in which the polypeptides are antibodies e.g. monoclonal, or antibody fragments, e.g. Fab. An antibody-metal/dioxime/boron complex conjugate of claim 12, which is immunoreactive and immunospecific toward a cancer target site. A composition for the diagnosis or the pharmaceutical treatment of cancer comprising a metal/dioxime/boron complex according to any of claims 1-5. Claims for the following Contracting States : ES, GR A process for the preparation of metal complexes of boronic acid adducts to dioxime ligands having formula XM[R 1 C(NOH)-CR 2 (NOH)] 3 B-(A 1 ) p -E or a pharmaceutically acceptable salt thereof, wherein: M is an isotope of technetium or rhenium; X is an anion, and R 1 and R 2 are each independently H, halogen, alkyl, aryl, or a 5- or 6-membered N-, S-, or O-containing heterocycle, or together R 1 and R 2 are -(CR 8 R 9 )n- where n = 3, 4, 5, or 6 and R 8 and R 9 are each independently H or alkyl; B is boron; (A1) is a chemical moiety which distances or otherwise isolates the moiety represented by "E" from the remainder of the complex II, p being zero or an integer up to 100, preferably 1 to 20; and E is a group of formula wherein R 3 is a function reactive toward amines and proteins selected from -NCS, -NCO, -O-Tosyl, -O-Mesityl or derivatized acyl halides, activated esters, and N-hydroxysuccinimide; and R 4 is H or a water solubilizing function selected from -OH, -COOH, -SO 3 H, alkyl sulfonates, -SO 2 NH 2 , -Alk-COOH, -Alk-OH, which method comprises: a) combining a Tc or Re source, a source of an anion, a boronic acid of formula VII (HO) 2 B(A 1 ) p -E, in which A 1 , p and E are defined above, and a dioxime of formula HO-N=CR 1 -CR 2 =N-OH in which R 1 and R 2 are defined above, or a pharmaceutically acceptable salt thereof; or b) reacting the foregoing boronic acid VII with a dioxime compound XM[R 1 C(NOH)-CR 2 (NOH)] 3 in which X, R1, R2 and M are as defined hereinbefore. The process of claim 1 in which A 1 is selected from alkylene, alkenylene, cycloalkylene, cycloalkenylene, oxaalkylene, hydroxyalkylene, carboxyalkylene, alkyleneamido, alkylenesulfonato, arylene, arylalkylene, heterocyclo, -CH 2 -, -CHR 6 -, -CR 6 R 10 -, -CH=CH-, -CH=CR 11 -, -CR 6 =CR 12 -, -C≡C-; -O-, -S-, -CO-, -HC=N-, -CR 6 =N-, or -NR 6 -, in which R 6 , R 10 , R 11 and R 12 are independently selected from alkyl, alkenyl, alkoxy, aryl, 5- or 6-membered N-containing heterocyclic rest, halogen, -OH, or hydroxyalkyl. The process of claim 1 in which the boron carrying moiety is of formula VI in which p = 0 or 1. The process of claims 1-3, wherein X is Cl - or OH - and the dioxime portion is dimethyl-glyoxime or a dioxime of ethane dione, 1,2-cyclopentane dione or 1,2-cyclohexane dione. The process of claims 1 for preparing Tc- or Re-(chlorine)(1,2-cyclohexanedione) 3 (3-carboxy-5-isothiocyanatophenylboron); Tc- or Re-(chlorine)(1,2-cyclohexanedione) 3 (3-isothiocyanatophenylboron); Tc- or Re-(chlorine)(dimethylglyoxime) 3 (3-carboxy-5-isothiocyanatophenylboron); Tc- or Re-(chlorine)(dimethylglyoxime) 3 (3-isothiocyanatophenylboron); Tc- or Re-(chlorine)(dimethylglyoxime) 3 (4-isothiocyanatophenylboron). A kit for labeling with 99m Tc or isotopic Re comprising, (i) a source of anion, e.g. Cl - ; (ii) a boronic acid compound of formula VIII in which A 1 is selected from alkylene, alkenylene, cycloalkylene, cycloalkenylene, oxaalkylene, hydroxyalkylene, carboxyalkylene, alkyleneamido, alkylenesulfonato, arylene, arylalkylene, heterocyclo, -CH 2 -, -CHR 6 -, -CR 6 R 10 -, -CH=CH-, -CH=CR 11 -, -CR 6 =CR 12 -, -C≡C-; -O-, -S-, -CO-, -HC=N-, -CR 6 =N-, or -NR 6 -, in which R 6 , R 10 , R 11 and R 12 are independently selected from alkyl, alkenyl, alkoxy, aryl, 5- or 6-membered N-containing heterocyclic rest, halogen, -OH, or hydroxyalkyl; p is 0 to 100, R 3 is a functional group selected from -NCS, -NCO, -O-Tosyl, -O-Mesityl or derivatized acyl halides, activated esters, or N-hydroxysuccinimide; and R 4 is H or a water solubilizing function selected from -OH, -COOH, -SO 3 H, alkyl-sulfonates, -SO 2 NH 2 , -Alk-COOH, -Alk-OH; (iii) a vic dioxime HO-N=CR 1 -CR 2 =N-OH or a salt thereof in which R 1 and R 2 are each independently H, halogen, alkyl, aryl, or a 5- or 6-membered N-, S-, or O-containing heterocycle, or together R 1 and R 2 are -(CR 8 R 9 ) n - where n = 3, 4, 5, or 6 and R 8 and R 9 are each independently H or alkyl; and (iv) optionally, a reducing agent. A kit according to claim 6, in which the dioxime is dimethyl-glyoxime or a dioxime of ethane dione, 1,2-cyclohexanedione-dioxime or 2,3-butanedione-dioxime; and the boronic acid is 3-carboxy-5-isothiocyanatophenylboronic acid or 3-isothiocyanatophenylboronic acid. A method of making a conjugate by the reaction of a metal/dioxime/boron complex from the process of claims 1-5 and an amine-containing compound, wherein said amine-containing compound is selected from proteins, e.g. fibrin, fibrinogen and low density lipoprotein, polypeptides, hormones, amine-containing steroids, aminoacids, or amine-containing drugs and compounds. The method of claim 8, in which the polypeptides are antibodies e.g. monoclonal, or antibody fragments, e.g. Fab. The method of claim 9, whereby there is obtained an antibody-metal/dioxime/boron complex conjugate immunoreactive and immunospecific toward a cancer target site.

Inventors: LINDER KAREN E, NUNN ADRIAN D, RAMALINGAM KONDAREDDIAR, LINDER, KAREN E., NUNN, ADRIAN D., RAMALINGAM, KONDAREDDIAR
Applicants: BRACCO INT BV, BRACCO INTERNATIONAL B.V.
Application year: 1991
Main classification: C07F13
IPC codes: G01N33, A61K51, C07F13, A61K47