O-methyl derivatives of azithromycin A, process and intermediates for their preparation, and use thereof in the preparation of pharmaceuticals

Abstract: The invention relates to O-methyl derivatives of azithromycin A of the formula (I) wherein Ia R¹ = R² = CO₂CH₂C₆H₅, R³ = CH₃, R⁴ = R⁵ = H Ib R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = CH₃, R⁵ = H Ic R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁵ = H, R⁴ = CH₃ Id R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = R⁵ = CH₃ Ie R¹ = R² = R⁴ = R⁵ = H, R³ = CH₃ If R¹ = R² = R⁵ = H, R³ = R⁴ = CH₃ Ig R¹ = R² = R³ = R⁵ = H, R⁴ = CH₃ Ih R¹ = R² = H, R³ = R⁴ = R⁵ = CH₃ Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ to their pharmaceutically acceptable addition salts with inorganic or organic acids, to a process for their preparation and to their use in the obtaining of antibacterial pharmaceutical preparations.

Description: This invention relates to novel, semisynthetic macrolide antibiotics of the azalide series, particularly to O-methyl derivatives of azithromycin A and to pharmaceutically acceptable addition salts thereof, to a process and intermediates for the preparation thereof, and to their use in the preparation of pharmaceuticals, which are particularly indicated as antimicrobial agents. Erythromycin A is a macrolide antibiotic, whose structure is characterized by a 14-membered aglicone ring, possessing a keto group in C-9 position (Bunch R.L. et al, US patent 2,653,899; 9/1953); it has been hitherto the leading macrolide antibiotic in the treatment of infections in humans. However, in acidic medium it is easily converted into anhydroerythromycin, which is an inactive C-6/C-12 metabolite of a spiroketal structure (Kurath P et al., Experientia 1971, 27 362). It has been known that the spiro-cyclisation of erythromycin A is successfully inhibited by means of a chemical transformation of C-9 ketone upon the obtaining of C-9 oximes (Djokic ′ S. et al., Tetrahedron Lett., 1967, 1945) or C-9(R) and C-9(S) amines (Egan R.S. et al, J.Org.Chem., 1974, 39, 2492), or by the elimination of the C-9 ketone upon the expansion of the aglycone ring (Kobrehel G. et al., US patent 4,328,334; 5/1982). Thus Beckmann rearrangement of erythromycin A oxime, followed by the reduction of the obtained imino ether (Djokic ′ S. et al, J.Chem.Soc.Perkin Trans 1, 1986, 1881) yielded the 11-aza-10-deoxo-10-dihydroerythomycin A (9-deoxo-9a-aza-9a-homoerythromycin A), which was the first 15-membered macrolide antibiotic of the azalide series. Upon methylation of the newly introduced secondary amino group in the aglycone ring with formaldehyde in the presence of formic acid via the modified Eschweiler-Clark procedure (Kobrehel G. and Djokic ′ S., BE patent 892,357; 7/1982), or upon preliminary protection of the amino groups by means of conversion into the corresponding N-oxides, followed by the alkylation and reduction of the obtained N-oxides (Bright G., US patent 4,474,768; 10/1984), there was obtained the N-methyl-11-aza-10-deoxo-10-dihydroerythromycin A (9-deoxo-9a-aza-9a-methyl-9a-homoerythromycin A) (IUPAC Nomenclature of Organic Chemistry, 1979, 68-70. 459, 500-503), which is being clinically tested under the non-proprietary name of azithromycin. In comparison with the parent antbiotic, azithromycin exhibits, in addition to an improved stability in acidic medium, also an improved in vitro activity against gram-negative microorganisms and a significantly higher conncentration in the tissues, and there is even being tested the possibility of a one-day dose (Ratshema J. et al, Antimicrob. Agents Chemother., 1987, 31, 1939). Further, it has been known that the C-6/C-12 spiro-cyclisation of erythromycin A is successfully inhibited by means of O-methylation of the hydroxy group in C-6 position of the aglycone ring (Watanabe Y. et al., US patent 4,331,803; 5/1982). The reaction of erythromycin A with benzyl chloroformate, followed by the methylation of the obtained 2'-0,3'-N-bis(benzyloxycarbonyl)-derivative, upon the elimination of the protective groups in positions 2'- and 3'- as well as the N-methylation of the 3'-methylamino group under reductive conditions, yields, in addition to 6-O-methyl-erythromycin A, also significant quantities of 11-O-methyl- and 6,11-di-O-methyl-erythromycin A (Morimoto S. et al, J. Antibiotics 1984, 37, 187). A higher selectivity is achieved by the preliminary oximation of the C-9 ketones and the O-methylation of the corresponding substituted or unsubstituted benzyloximino derivatives (Morimoto S. et al, US patent 4,680,368; 7/1987). 6-O-methyl-erythromycin A is being clinically tested under the non-proprietary name of clarithromycin. In comparison to erythromycin A, clarithromycin exhibits an improved in vitro activity against gram-positive microorganisms (Kirist H.A. et al, Antimicrobial Agents and Chemother., 1989, 1419). The Applicant's search has revealed no disclosure on O-methyl derivatives of azithromycin A in the State of the Art. Hence the first object of the present invention are new O-methyl derivatives of azithromycin A of the formula (I) wherein Ia R¹ = R² = CO₂CH₂C₆H₅, R³ = CH₃, R⁴ = R⁵ = H Ib R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = CH₃, R⁵ = H Ic R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁵ = H, R⁴ = CH₃ Id R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = R⁵ = CH₃ Ie R¹ = R² = R⁴ = R⁵ = H, R³ = CH₃ If R¹ = R² = R ⁵= H, R³ = R⁴ = CH₃ Ig R¹ = R² = R³ = R⁵ = H, R⁴ = CH₃ Ih R¹ = R² = H, R³ = R⁴ = R⁵ = CH₃ Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ and their pharmaceutically acceptable acid addition salts. A further object of the present invention is a process for the preparation of O-methyl derivatives of azithromycin A of the formula (I) and of their pharmaceutically acceptable acid addition salts, wherein azithromycin or its dihydrate (Djokic ′ S. et al, J. Chem. Research (S), 1988, 152-153; (M) 1988 1239-12621) of the formula (II) wherein IIa R¹ = H, R² = CH₃, is reacted with benzyl chloroformate in the presence of an excess of a suitable base, e.g. sodium hydrogen carbonate, in a reaction inert solvent, e.g. benzene, at a temperature of 25 °C to 60 °C, within 3 to 24 hours, depending on the reaction temperature, followed by O-methylation of the hydroxy groups in the C-6, C-11 and C-4'' positions of a new, as yet undisclosed intermediate 2'-0,3'-N-bis-(benzyloxycarbonyl)-N-demethyl-azithromycin A of the formula (II), wherein IIb R¹ = R² = CO₂CH₂C₆H₅, with a 1-18 molar excess of an appropriate methylation agent, e.g. methyl iodide, dimethyl sulfate, methyl methanesulfonate or methyl p -toluenesulfonate, in the presence of an appropriate base, e.g. sodium hydride, aqueous potassium hydroxide or sodium hydroxide, in an appropriate solvent, e.g. dimethyl sulfoxide or N,N-dimethyl-formamide, or their mixtures with a reaction inert solvent, e.g. tetrahydrofurane, acetonitrile, ethyl acetate, 1,2-dimethoxyethane, at a temperature of 0 °C to room temperature, within 3 to 30 hours, yielding a mixture of O-methyl-2'-0,3'-N-bis-(benzyloxycarbonyl)-N-demethyl-azithromycin A of the formula (I), wherein Ia R¹ = R² = CO₂CH₂C₆H₅, R³ = CH₃, R⁴ = R⁵ = H Ib R ¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = CH₃, R⁵ = H Ic R ¹ = R² = CO₂CH₂C₆H₅, R³ = R⁵ = H, R⁴ = CH₃ Id R ¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = R⁵ = CH₃ which is optionally subjected to A) separation on a silica gel column (Silica gel 60, Merck Co., 70-230 mesh) with the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:0.5), yielding chromatographically homogenous compounds (Ia) of an R f 0.660, (Ib) of an R f 0.811, (1c) of an R f 0.843 and (Id) of an R f 0.881, which are subsequently subjected to the elimination of the protecting benzyloxycarbonyl groups in positions 2'- and 3'- by means of hydrogenolysis in a solution of lower alcohols, e.g. methanol or ethanol, in the presence of a catalyst, e.g. palladium black or palladium-on-carbon, in a hydrogen atmosphere at a pressure of 1-20 bar, under stirring of the reaction mixture, within 2-10 hours, at room temperature, yielding, upon filtration of the catalyst and the isolation of the product by means of conventional pH-gradient extraction methods (pH 5.0 and pH 9.0) from water with an appropriate hydrophobic solvent, e.g. chloroform, dichloromethane, ethyl acetate etc., the O-methyl-N-demethyl-azithromycin A derivatives of formula (I), wherein Ie R¹ = R² = R⁴ = R⁵ = H, R³ = CH₃ If R¹ = R² = R ⁵= H, R³ = R⁴ = CH₃ Ig R¹ = R² = R³ = R⁵ = H, R⁴ = CH₃ Ih R¹ = R² = H, R³ = R⁴ = R⁵ = CH₃ which are then subjected to reductive N-methylation of the 3'-methylamino group with 1-3 equivalents of formaldehyde (37%) in the presence of an equal or double quantity of formic acid (98-100%) or another hydrogen source, in a reaction inert solvent chosen from halogenated hydrocarbons, e.g. chloroform, or lower alcohols, e.g. methanol or ethanol, lower ketones, e.g. acetone, at reflux temperature of the reaction mixture within 2 to 8 hours, yielding upon the isolation of the product by means of conventional pH-gradient extraction methods (pH 5.0 and pH 9.0) O-methyl-azithromycin A derivatives of formula (I), wherein Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ or B) elimination of the protecting benzyloxycarbonyl group in 2'- and 3'-positions by hydrogenolysis as described in A), yielding a mixture of 6-O-methyl- (Ie), 6,11-di-O-methyl- (If), 11-O-methyl- (Ig) and 6,11,4''-tri-O-methyl-N-demethyl-azithromycin A (Ih), which is subjected to reductive N-methylation with formaldehyde (37%) in the presence of formic acid (98-100%) or some other hydrogen source, as described in A), yielding a mixture of 6-O-methyl- (Ii), 6,11-di-O-methyl-(Ij), 11-O-methyl- (Ik) and 6,11,4''-tri-O-methyl-azithromycin A (Il), which is subjected to separation on a silica gel column with the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:0.5), yielding chromatographically homogenous (TLC, the same solvent system) O-methyl derivatives of azithromycin A (Ii) of an R f 0.346, (Ij) of an R f 0.393, (Ik) of an R f 0.428 and (Il) of an R f 0.456. Pharmaceutically acceptable addition salts of the compounds of formula (I) are obtainable by reacting O-methyl derivatives of azithromycin A (I) with at least an equimolar quantity of a corresponding organic or inorganic acid, chosen e.g. from hydrogen chloride, hydrogen iodide, sulphuric acid, phosphoric acid, acetic acid, propionic acid, trifluoroacetic acid, maleic acid, citric acid, ethyl succinic acid, succinic acid, methanesulfonic acid, benzenesulfonic acid, p -toluenesulfonic acid, lauryl- sulfonic acid and the like, in a reaction inert solvent. The addition salts are isolated by filtration if they are insoluble in the applied reaction inert solvent, or by precipitation by means of a non-solvent, most often by means of a lyophilization procedure. O-methyl derivatives of azithromycin A of the formulae (Ii)-(Il) and their pharmaceutically acceptable addition salts have a potent antimicrobial activity. The preliminary antibacterial in vitro activity of 6-O-methyl-azithromycin A (Ii) was determined on a series of gram-positive and gram-negative test bacteria and clinical isolates in comparison with erythromycin A. The assessment was performed by the "tube dilution" method. In the investigation there were used 24-hours cultures in "brain hearth bouillon " of standard strains and freshly isolated strains from a clinical sample. The results are expressed as Minimal Inhibitory Concentration or Bactericidal Concentration (MIC and MBC resp. in µg/mL) and represented in Tables 1 and 2, and they show that 6-O-methyl-azithromycin A has a somewhat improved activity on the investigated strains in comparison with erythromycin A. In Table 3 there are represented in vitro tests of 6-O-methyl- (Ii), 6,11-di-O-methyl- (Ij), 11-O-methyl- (Ik) and 6,11,4''-tri-O-methyl-azithromycin A (Il) in comparison with azithromycin. Minimal Inhibitory Concentrations (MIC; µg/mL) determined on a series of standard bacterial strains show that 6-O-methyl-azithromycin A (Ii) is twice as active on Bacillus subtilis NCTC 8241 and Sarcina lutea ATCC 9341, and four times as active on Micrococcus flavus ATCC 6538 P with respect to azithromycin. A significantly higher activity was also exhibited by 11-O-methyl-azithromycin A (Ik). Namely, the majority of the investigated bacterial strains was 2 to 4 times more sensitive in comparison with the parent antibiotic. It is a further object of the present invention to provide pharmaceuticals comprising an effective, yet physiologically acceptable dose of the novel compounds of the present invention. Compounds (Ii)-(Il) as well as their pharmaceutically acceptable salts may be used as therapeutical agents in the treatment of human or animal infectious diseases caused by gram-positive bacteria, mycoplasmas or patogenous bacteria, which are sensitive to compounds (Ii)-(Il). Thus, the compounds (Ii)-(Il) and their pharmaceutically acceptable addition salts may be administered orally or parenterally, e.g. in the form of s . c . or i . m . injections, tablets, capsules, powders and the like, formulated in accordance with the conventional pharmaceutical practice. Antibacterial in vitro activity of 6-O-methyl-azithromycin A (Ii) in comparison with erythromycin A Test Organism Erythromycin A 6-O-methyl-azithromycin A (Ii) MIC MBC MIC MBC Staphilococcus aureus ATCC 6538-P 0.2 0.8 0.2 0.4 Streptococcus faecalis ATCC-8043 0.2 0.8 0.2 0.4 Sarcina lutea ATCC-9341 0.2 0.4 0.1 0.2 Escherichia coli ATCC 10536 50 >50 1.6 3.2 Klebsiella pneumoniae NCTC-10499 >50 >50 12.5 50 Pseudomonas aeruginosa NCTC-10490 >50 >50 >50 >50 Substrate: Brain hearth bouillon Incubation: 24 hours, 37 °C MIC: Minimal Inhibitory Concentration (µg/mL) MBC: Minimal Bactericidal Concentration (µg/mL) Antibacterial in vitro activity of 6-O-methyl-azithromycin A (Ii) in comparison with erythromycin A against clinical isolates Test Organism Erythromycin A 6-O-methyl-azithromycin A (Ii) MIC MBC MIC MBC Staphylococcus aureus 10099 0.1 0.2 0.05 0.1 Staphylococcus saprophyticus 3947 0.4 0.8 0.4 0.8 Streptococcus faecalis 10390 0.8 3.1 0.8 3.1 Staphylococcus aureus 10097 0.1 0.4 0.05 0.4 Streptococcus pneumoniae 4050 0.1 0.4 0.025 0.1 Haemophylus influenzae 4028 0.05 0.2 0.05 0.2 Substrate: Brain hearth bouillon Incubation: 24 hours, 37 °C MIC: Minimal Inhibitory Concentration (µg/mL) MBC: Minimal Bactericidal Concentration (µg/mL) Antibacterial in vitro activity of the novel O-methyl-azithromycin A derivatives in comparison with azithromycin Test Strain MIC (µg/mL) (IIa) (Ii) (Ij) (Ik) (Il) Micrococcus flavus ATCC 6538P 1.56 0.39 1.56 0.2 3.125 Corynebacterium xerosis NCTC 9755 6.25 12.5 12.5 1.56 25.0 Staphylococcus aureus ATCC 10240 0.39 0.79 0.78 0.1 3.125 Bacillus subtilis NCTC 8241 0.39 0.2 0.78 0.1 3.125 Bacillus pumilus NCTC 8241 0.2 0.2 0.78 0.05 3.125 Bacillus cereus NCTC 10320 0.39 0.78 1.56 0.1 3.125 Sarcina lutea ATCC 9341 0.05 0.0125 0.05 0.0125 0.05 Staphylococcus epidermidis ATCC 12228 0.1 0.1 1.56 0.1 3.125 Staphylococcus faecalis ATCC 8043 0.05 0.05 0.78 0.05 0.78 Pseudomonas aeruginosa NCTC 10490 100.0 100.0 100.0 25.0 200.0 Escherichia coli ATCC 10536 0.78 3.125 6.25 0.78 6.25 Substrate: Brain hearth Bouillon Incubation: 24-48 hours, 37 °C Inocculum; 10⁻⁵-10⁻⁶ cfu/mL The invention is illustrated by the following Examples. EXAMPLE 1 2'-0,3'-N-Bis(benzyloxycarbonyl)-N-demethyl-azithromycin A (IIb) Method A Upon the addition of NaHCO₃ (48g) into a solution of azithromycin dihydrate (30g; 0.038 mole) in 140 mL of dry benzene the reaction mixture was heated under stirring to 55-60 °C, whereupon there were added drop by drop gradually within 1 hour 75 mL (89.63 g; 0.53 mole) of benzyl chloroformate. The reaction mixture was kept stirring at this temperature for 3 hours and left standing overnight at room temperature. The benzene suspension was extracted three times with 150 mL of 0.25 N HCl, the benzene solution was dried over CaCl₂, filtered, and evaporated at reduced pressure into a thick oil. The obtained residue was added drop-by-drop under thorough stirring into 500 mL of cooled petrolether, the reaction suspension was stirred under cooling for 4 hours, the precipitate was filtered, washed with petroleum ether and dried, yielding 27.5 g (71.6%) of the title product, which upon recrystallization from ether/petroleum ether yielded a product of a m.p. 148-154 °C. EI-MS m/s 1003 (M⁺) TLC, CH₂Cl₂/CH₃OH/NH₄OH (90:9:0.5) R f 0.704 IR (CHCl₃): 3510, 3350, 2960, 1740, 1690, 1605, 1450, 1380, 1330, 1290, 1255, 1160, 1115, 1050, 995 cm⁻¹. ¹H NMR (CDCl₃): 2.301 (3H, 9a-NCH₃), 2.844, 2.802 (3H, 3'-NCH₃), 3.397 (3H, 3''-OCH₃). ¹³C NMR (CDCl₃): 177.260 (C-1), 100.115 (C-1'), 95.149 (C-1''), 75.028 (C-6), 74.607 (C-12), 69.415 (C-9), 64.617 (C-10), 36.964 (9a-NCH₃) and 26.016 (C-8) ppm. Method B Upon the addition of NaHCO₃ (22 g) under stirring into a solution of benzyl chloroformate (30mL; 0.21 mole) in 50 mL of dry benzene, there were added gradually within 3 hours 15 g (0.019 mole) of azithromycin. At the moment of the addition of about 3/4 of the total amount of azithromycin, there was added a further quantity of 15 mL (0.106 mole) of benzyl chloroformate. The reaction mixture was kept under stirring for 24 hours at room temperature, filtered, whereupon the filtrate was extracted three times with 150 mL of 0.25 N HCl, dried over MgSO₄, and evaporated at reduced pressure. Upon the addition of petroleum ether the crude 2'-0,3'-N-bis-(benzyloxycarbonyl)-N-demethyl-azithromycin A was precipitated, the obtained precipitate was filtered and immediately suspended under stirring in 50 mL of cold ether. The reaction suspension was stirred at room temperature for 1 hour, the precipitate was filtered and dried, yielding 8.67 g (43.09%) of a homogeneous product (TLC) of identical physical- chemical characteristics as cited above in Method A). EXAMPLE 2 O-Methylation of 2'-0,3'-N-bis(benzyloxycarbonyl)-N-demethyl-azithromycin A (Ia), (Ib), (Ic) and (Id) Method A Upon the addition of methyl iodide (6 mL; 0.106 mole) into a solution of the product of Example 1 (6 g; 0.006 mole) in 64 mL of dimethyl sulfoxide and tetrahydrofurane (1:1), there were added methyl iodide (6.6 mL; 0.106 mole) and then, gradually within 4 hours at room temperature, 2.4 g (approx. 0.06 mole) of NaH (55-60%) in oil. The reaction suspension was stirred for further 5 hours, left standing overnight, poured into a saturated NaCl solution (100 mL), and extracted twice with 100 mL of ethyl acetate. The combined organic extracts were washed three times with 100 mL of saturated NaCl solution, dried over K₂CO₃, and evaporated, yielding 6.35 g of a crude product, which was subjected to hydrogenolysis in accordance with the process described in Example 9 or, optionally, to purification by means of chromatography on a silica gel column (Silica gel 60, Merck Co., 70-230 mesh), using the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90: 9:0.5). From 1.5 g of the crude product there were obtained, upon the concentration and evaporation of the fractions of R f 0.881 (TLC; identical solvent system), 0.12 g of the chromatographically pure 2'-0,3'-N-bis(benzyloxycarbonyl)-N-demethyl-6,11,4''-tri-O-methyl-azithromycin A (Id): ¹H NMR (CDCl₃): 2.246 (3H, 9a-NCH₃), 2.831, 2.798 (3H, 3'-NCH₃), 3.367 (3H, 3''-OCH₃), 3.305 (3H, 6-OMe), 3.465 (3H, 4''-OCH₃), and 3.485 (3H, 11-OCH₃) ppm. ¹³C NMR (CDCl₃): 176.975 (C-1), 69.920 (C-9), 35.967 (9a-NCH₃), 79.1 (C-6). 52.8 (6-OCH₃), 89.0 (C-11), 62.0 (11-OCH₃). 87.357 (C-4''), 61.131 (4''-OCH₃), 49.176 and 49.526 (3''-OCH₃) and 36.457 (3'-NCH₃) ppm. Upon the combination and evaporation of the fractions of R f 0.843, 0.32g of the chromatographycally pure 2'-0,3'-N-bis(benzyloxycarbonyl)-N-demethyl-11-O-methyl-azithromycin A (Ic) were obtained: EI-MS m/s 1016 (M⁺) ¹H NMR (CDCl₃): 2.239 (3H, 9a-NCH₃), 2.805, 2.847 (3H, 3'-NCH₃), 3.374 (3H, 3''-OCH₃), and 3.573 (3H, 11-OCH₃) ppm. Upon the evaporation of the fractions of R f 0.811, 0.316g of 2'-0,3'-N-bis(benzyloxycarbonyl)-N-demethyl-6,11-di-O-methyl-azithromycin A (Ib) were obtained: IR (CHCl₃): 3570, 3490, 1740, 1690, 1455, 1380, 1330, 1295, 1260, 1200, 1160, 1120, 1095, 1055, 1005, 990, 980 cm⁻¹. ¹H NMR (CDCl₃): 2.292 (3H, 9a-NCH₃), 2.838, 2.795 (3H, 3'-NCH₃), 3.380 (6H, 6-OCH₃ and 3''-OCH₃) and 3.488 (3H, 11-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.939 (C-1), 69.471 (C-9), 35.271 (9a-NCH₃), 88.994 (C-11), 52.892 (6-OCH₃), 61.09 (11-OCH₃), 36.851 (3'-NCH₃), and 49.549, 49.154 (3''-OCH₃) ppm. Upon concentration and evaporation to dryness of the fractions of R f 0.661, 0.384 g of 2'-0,3'-N-bis(benzyloxycarbonyl)-N-demethyl-6-O-methyl-azithromycin A (Ia) were obtained: EI-MS m/s 1016 (M⁺) IR (CHCl₃): 3570, 3500, 2960, 2920, 1740, 1690, 1450, 1380, 1325, 1290, 1255, 1200, 1160, 1120, 1050, 995 cm⁻¹. ¹H NMR (CDCl₃): 2.288 (3H, 9a-NCH₃), 2.805, 2.847 (3H, 3'-NCH₃), 3.380 (6H, 6-OCH₃ and 3''-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.764 (C-1), 69.850 (C-9), 34.851 (9a-NCH₃), 78.106 (C-6), 74.661 (C-11),, 73.873 (C-12), and 52.822 (6-OCH₃) ppm. Method B Into a solution of the product of Example 1 (6 g) in 60 mL of dimethyl sulfoxide and tetrahydrofurane (1:1), there was added, under stirring, gradually within 2 hours at a temperature of 0-5 °C methyl iodide (3 mL) and 2.1 g of NaH (55-60%). The reaction mixture was stirred for 1 hour at 0-5 °C, the suspension was poured on a saturated NaCl solution and extracted with ethyl acetate. The organic extracts were washed with a saturated NaCl solution, dried over K₂CO₃, and evaporated to dryness at reduced pressure. The obtained product (2 g) was subjected to purification by means of chromatography on a silica gel column, using the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:1.5) and yielding 0.89 g of 6-O-methyl derivative (Ia), 0.11 g of 6,11-di-O-methyl derivative (Ib) and 0.48 g of 11-O-methyl derivative (Ic). Method C Upon the addition of methyl iodide (6 mL) into a solution of the product of Example 1 (6 g) in 60 mL of N,N-dimethylformamide, there were added, under stirring, gradually within 2 hours at room temperature 2.4 g of NaH (55-60%). The reaction mixture was stirred for further 2 hours at said temperature and left overnight. Upon the isolation of the product in accordance with the procedure described in Method A), there were obtained 4.54 g of a mixture of 6,11-di-O-methyl derivative (Ib) and 6,11,4''-tri-O-methyl derivative (Id). This mixture was subjected to hydrogenolysis in methanol (60 mL) in the presence of a NaOAc/HOAc buffer (pH 5) and palladium-on-carbon (2g; 5%) as catalyst, according to the procedure described in Example 3. Upon the isolation of the product and evaporation of the solvent at pH 9.0 there was isolated a mixture (2.33g) of 6,11-di-O-methyl-N-demethyl-azithromycin A (If) of R f 0.220 and 6,11,4''-tri-O-methyl-N-demethyl-azithromycin A (Ih) of R f 0.263, which upon separation on a silica gel column in the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:1), yielded a chromatographically homogeneous product (If) and (Ih). EXAMPLE 3 6-O-Methyl-N-demethyl-azithromycin A (Ie) 2.0 g (0.002 mole) of 2'-0,3'-N-bis(benzyl-oxycarbonyl)-N-demethyl-6-O-methyl-azithromycin A (Ia) were dissolved in 30 mL of ethanol. 10 mL of water, which contained 0.185 mL of acetic acid and 0.3g of sodium acetate (pH 5) and 0.7g of palladium-on-carbon (10%) was charged into the solution. The reaction mixture was stirred under hydrogen pressure (10 bar) for 10 hours, the catalyst was filtered and evaporated to dryness. The residue was dissolved in CHCl₃ (30 mL) and upon the addition of water (30 mL) and adjustment of the pH of the reaction mixture with 1N HCl to 5.0, the layers were separated and the aqueous layer was extracted twice with CHCl₃ (each time with 15 mL). To the reaction mixture CHCl₃ (30 mL) was added, the pH was adjusted to 9.0 under stirring with 2N NaOH, the layers were separated, and the aqueous layer was again extracted twice with CHCl₃ (each time with 15 mL). The combined organic extracts (pH 9.0) were dried over K₂CO₃, filtered and evaporated to yield 1.03 g (70%) of the title product: EI-MS m/s 748 TLC, R f 0.182 IR (CHCl₃): 3670, 3500, 2960, 2920, 1725, 1460, 1375, 1345, 1320, 1280, 1260, 1165, 1120, 1085, 1045, 1010, 995, 900 cm⁻¹. ¹H NMR (CDCl₃): 2.278 (3H, 9a-NCH₃), 2.406 (3H, 3'-NCH₃), 3.312 (3H, 3''-OCH₃), 3.384 (3H, 6-OCH₃) ppm. EXAMPLE 4 6,11-Di-O-Methyl-N-demethyl-azithromycin A (If) In accordance with the procedure of Example 3, from 0.165 g (0.16 mole) of 2'-0,3'-N-bis- (benzyloxycarbonyl)-N-demethyl-6,11-di-O-methyl-azithromycin A (Ib) by means of hydrogenolysis with palladium-on-carbon (10%) in ethanol in the presence of the buffer sodium acetate/acetic acid (pH 5.0), there were obtained 0.093 g (76,2%) of the chromatographically homogeneous title product; m.p. 95-98 °C. EI-MS m/s 762 TLC, R f 0.331 ¹H NMR (CDCl₃): 2.265 (3H, 9a-CH₃), 2.422, (3H, 3'-NCH₃), 3.312 (3H, 3''-OCH₃), 3.374 (3H, 6-OCH₃) and 3.521 (3H, 11-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.7 (C-1), 65.9 (C-9), 36.8 (9a-NCH₃), 79.3 (C-6), 88.9 (C-11), 52.7 (6-OCH₃), 62.0 (11-OCH₃), 33.1 (3'-NCH₃), and 49.7 (3''-OCH₃) ppm. EXAMPLE 5 11-O-Methyl-N-demethyl-azithromycin A (Ig) In accordance with the procedure of Example 3, from 0.250 g (0.246 mmole) of 2'-0,3'-N-bis(benzyloxycarbonyl)-N-demethyl-11-O-methyl-azithromycin A (Ic) by means of hydrogenolysis with palladium-on-carbon (10%) in methanol in the presence of the buffer sodium acetate/acetic acid (pH 5.0), there were obtained 0.168 g (89,5%) of 11-0-methyl-N-demethyl-azithromycin A (Ig): TLC, R f 0.244 IR (CDCl₃): 3500, 2970, 2940, 1736, 1460, 1380, 1165 cm⁻¹. ¹H NMR (CDCl₃): 2.44 (3H, 9a-NCH₃), 2.458, (3H, 3'-NCH₃), 3.336 (3H, 3''-OCH₃) and 3.590 (3H, 11-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.6 (C-1), 70.7 (C-9), 35.8 (9a-NCH₃), 74.4 (C-6), 85.0 (C-11), 62.7 (11-OCH₃), 36.7 (3'-NCH₃), and 49.4 (3''-OCH₃) ppm. EXAMPLE 6 6-O-Methyl-azithromycin A (Ii) Method A Into a solution of 0.78 g (0.00104 mole) of 6-O-Methyl-N-demethyl-azithromycin A (Ie) in CHCl₃ (50 mL) there were added 0.085 mL (0.00113 mole) of formaldehyde (37%) and 0.078 mL (0.00203 mole) of formic acid (98-100%). The reaction mixture was stirred under reflux for 8 hours, cooled to room temperature, and poured onto 50 mL of water. Upon the adjustment of the pH of the reaction mixture with 1N HCl to 5.0, the layers were separated and the aqueous layer was extracted twice with CHCl₃ (each time with 20 mL). To the aqueous portion CHCl₃ (20 mL) was added, the pH was adjusted to 9.0 under stirring with 2N NaOH, the layers were separated, and the aqueous layer was again extracted twice with CHCl₃ (each time with 20 mL). The combined CHCl₃ extracts (pH 9.0) were dried over K₂CO₃ and evaporated to yield 0.495 g (62,74%) of the title product, which was optionally purified by chromatography on a silica gel column, using the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:0.5) and yielding chromatographically homogeneous (Ii), m.p. 103-109 °C. EI-MS m/s 762 TLC, R f O.346 IR (KBr): 3500, 2980, 2940, 1740, 1462, 1385, 1330, 1280, 1260, 1170, 1112, 1059, 1018, and 1055 cm⁻¹. ¹H NMR (CDCl₃): 2.300 (3H, 9a-NCH₃), 2.316 (6H, 3'-N(CH₃)₂), 3.333 (3H, 3''-OCH₃) and 3.384 (3H, 6-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.540 (C-1), 68.850 (C-9), 36.8 (9a-NCH₃), 79.2 (C-6), 52.822 (6-OCH₃), 61.627 (C-10), 40.350 (3'-N(CH₃)₂) and 49.457 (3''-OCH₃) ppm. Biological activity: 1 mg contains 754 µg of azithromycin. Method B Into a solution of 0.5 g (0.668 mmole) of 6-O-methyl-N-demethyl-azithromycin A in acetone (30 mL) there were added 0.128 mL (1.71 mmole) of formaldehyde (37%) and 0.118 mL (3.06 mmole) of formic acid (98-100%), and it was refluxed under stirring for 2 hours. The reaction mixture was cooled to room temperature and acetone was evaporated to yield a thick syrup and upon addition of 20 mL of water the product was isolated by means of gradient pH extraction by means of methylene chloride as described in Method A). Yield: 0.46 g (90.3%). EXAMPLE 7 6,11-Di-O-Methyl-azithromycin A (Ij) In accordance with the procedure of Example 6, from 0.49 g (6.43 mmole) of 6,11-di-O-methyl-N-demethylazithromycin A (If) by means of reductive N-methylation with formaldehyde (37%; 0.083 mL) in the presence of formic acid (98-100%), there were obtained 0.46 g (92,3%) of the title product: EI-MS m/s 776 (M⁺) TLC, R f O.391 ¹H NMR (CDCl₃): 2.295 (3H, 9a-NCH₃), 2.316 (6H, 3'-N(CH₃)₂), 3.321 (3H, 3''-OCH₃) 3.38 (3H, 6-OCH₃) and 3.524 (3H, 11-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.540 (C-1), 68.237 (C-9), 36.739 (9a-NCH₃), 88.112 (C-11), 52.653 (6-OCH₃) and 61.852 (11-OCH₃) ppm. EXAMPLE 8 11-O-Methyl-azithromycin A (Ik) In accordance with the procedure of Example 6, from 0.32 g (0.43 mmole) of 11-O-methyl-N-demethyl-azithromycin A (Ig) by means of reductive methylation with formaldehyde (37%) in the presence of formic acid (98-100%), there were obtained 0.238 g (72,44%) of the title 11-O-methyl derivative (Ik): EI-MS m/s 762 (M⁺) TLC, R f O.428 IR (KBr): 3510, 2975, 2940, 1738, 1460, 1350, 1165, 1054 cm⁻¹. ¹H NMR (CDCl₃): 2.246 (3H, 9a-NCH₃), 2.307 (6H, 3'-N(CH₃)₂), 3.352 (3H, 3''-OCH₃) and 3.591 (3H, 11-OCH₃) ppm. EXAMPLE 9 6-O-Methyl-azithromycin A (Ii), 6,11-di-O-methyl-azithromycin A (Ij), 11-O-methyl-azithro- mycin A (Ik) and 6,11,4''-tri-O-methyl-azithromycin A (Il) 1) Into a solution of 2.16 g of the crude product of Example 2 in 30 mL of ethanol, there were added 10 mL of water containing 0.185 mL of acetic acid and 0.3 g of sodium acetate and 0.7 g of palladium-on-carbon (10%), whereupon the reaction mixture was subjected to hydrolysis, as described in Example 3. At a pH of 9.0 there were obtained 0.98 g of a mixture of 6-O-methyl- (Ie), 6,11-di-O-methyl- (If), 11-O-methyl- (Ig), and 6,11,4''-tri-O-methyl-N- demethyl-azithromycin A (Ih). 2) Upon the dissolving of 0.98 g of the mixture obtained as described in (1), in CHCl₃ (50 mL), there were added 0.106 mL of formaldehyde (37%) and 0.096 mL of formic acid (98-100%) and it was subjected to N-methylation as described in Example 6. At a pH of 9.0 there were isolated 0.537 g of a mixture, which was subjected to chromatography on a silica gel column (Silica gel 60, Merck Co., 70-230 mesh), using the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:1.5), and yielding 0.238 g of a chromatographically homogeneous (Ii) of R f 0.346, 0.065 g of (Ij) of R f 0.391, 0.105 g (Ik) of R f 0.428 and 0.094 g (Il) of R f 0.456. EXAMPLE 10 6,11,4''-Tri-O-Methyl-N-demethyl-azithromycin A (Ih) In accordance with the procedure of Example 3, from 3.35 g (3.21 mmole) of 2'-0,3'-N-bis-(benzyloxycarbonyl)-N-demethyl-6,11,4''-tri-O-methyl-azithromycin A (Id) by means of hydrogenolysis with palladium-on-carbon (10%; 1g) in ethanol (50mL) in the presence of the buffer sodium acetate/acetic acid (pH 5.0), there were obtained 1.41 g (56,7%) of the title product, which was optionally subjected to chromatography on a silica gel column using the solvent system CH₂Cl₂/CH₃OH/NH₄OH (90:9:0.5), and yielding a TLC homogeneous product (Ih): EI-MS m/s 775 TLC, R f O.263 ¹H NMR (CDCl₃): 2.262 (3H, 9a-NCH₃), 2.393 (3H, 3'-NCH₃), 3.308 (6H, 3''-OCH₃ and 6-OCH₃), 3.475 (4''-OCH₃) and 3.521 (11-OCH₃) ppm. ¹³C NMR (CDCl₃): 175.0 (C-1), 64.8 (C-9), 79.8 (C-6), 50.6 (6-OCH₃) 86.1 (C-11), 59.1 (11-OCH₃), 87.7 (C-4'') and 60.9 (4''-OCH₃) ppm. EXAMPLE 11 6,11,4''Tri-O-Methyl-azithromycin A (Il) In accordance with the procedure of Example 6, from 1.2 g (1.55 mmole) of 6,11,4''-tri-O- methyl-N-demethyl-azithromycin A (Ih), 0.131 mL of formaldehyde (37%; 1.71 mmole) and 0.121 mL (3.15 mmole) of formic acid (98-100%), there were obtained 0.75g (64,4%) of the title product. EI-MS m/s 789 TLC, R f O.456 ¹H NMR (CDCl₃): 2.216 (3H, 9a-NCH₃), 2.311 (6H, 3'-N(CH₃)₂), 3.321 (3H, 3''-OCH₃), 3.302 (6-OCH₃), 3.482 (4''-OCH₃) and 3.521 (11-OCH₃) ppm. ¹³C NMR (CDCl₃): 177.859 (C-1), 68.6 (C-9), 36.8 (9a-NCH₃), 80.7 (C-6), 51.0 (6-OCH₃), 89.0 (C-11), 62.0 (11-OCH₃), 87.3 (C-4'') and 61.3 (4''-OCH₃) ppm.

Claims: O-methyl derivatives of azithromycin A of the formula (I) wherein Ia R¹ = R² = CO₂CH₂C₆H₅, R³ = CH₃, R⁴ = R⁵ = H Ib R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = CH₃, R⁵ = H Ic R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁵ = H, R⁴ = CH₃ Id R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = R⁵ = CH₃ Ie R¹ = R² = R⁴ = R⁵ = H, R³ = CH₃ If R¹ = R² = R ⁵= H, R³ = R⁴ = CH₃ Ig R¹ = R² = R³ = R⁵ = H, R⁴ = CH₃ Ih R¹ = R² = H, R³ = R⁴ = R⁵ = CH₃ Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ and their pharmaceutically acceptable addition salts with inorganic or organic acids. Substance as claimed in claim 1, characterized in that R¹ and R² are identical and stand for a benzyloxycarbonyl group, R³ is CH₃, whereas R⁴ and R⁵ stand for hydrogen. Substance as claimed in claim 1, characterized in that R¹ and R² are identical and stand for a benzyloxycarbonyl group, R³ and R⁴ are CH₃, whereas R⁵ stands for hydrogen. Substance as claimed in claim 1, characterized in that R¹ and R² are identical and stand for a benzyloxycarbonyl group, R³ and R⁵ are hydrogen, whereas R⁴ stands for CH₃. Substance as claimed in claim 1, characterized in that R¹ and R² are identical and stand for a benzyloxycarbonyl group, whereas R³, R⁴ and R⁵ are CH₃. Substance as claimed in claim 1, characterized in that R¹, R², R⁴ and R⁵ are identical and stand for hydrogen, whereas R³ stands for CH₃. Substance as claimed in claim 1, characterized in that R¹, R², and R⁵ are identical and stand for hydrogen, whereas R³ and R⁴ stand for CH₃. Substance as claimed in claim 1, characterized in that R¹, R², R³ and R⁵ are identical and stand for hydrogen, whereas R⁴ stands for CH₃. Substance as claimed in claim 1, characterized in that R¹ and R² are identical and stand for hydrogen, whereas R³, R⁴ and R⁵ stand for CH₃. Substance as claimed in claim 1, characterized in that R¹, R⁴, and R⁵ are identical and stand for hydrogen, whereas R² and R³ stand for CH₃. Substance as claimed in claim 1, characterized in that R¹ and R⁵ are identical and stand for hydrogen, whereas R², R³ and R⁴ stand for CH₃. Substance as claimed in claim 1, characterized in that R¹, R³, and R⁵ are identical and stand for hydrogen, whereas R² and R⁴ stand for CH₃. Substance as claimed in claim 1, characterized in that R¹ stands for hydrogen, whereas R², R³, R⁴ and R⁵ are identical and stand for CH₃. Substance of formula (II) wherein (IIb) R¹ = R² = CO₂CH₂C₆H₅. A process for the preparation of O-methyl derivatives of azithromycin A of the formula (I) wherein Ia R¹ = R² = CO₂CH₂C₆H₅, R³ = CH₃, R⁴ = R⁵ = H Ib R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = CH₃, R⁵ = H Ic R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁵ = H, R⁴ = CH₃ Id R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = R⁵ = CH₃ Ie R¹ = R² = R⁴ = R⁵ = H, R³ = CH₃ If R¹ = R² = R ⁵= H, R³ = R⁴ = CH₃ Ig R¹ = R² = R³ = R⁵ = H, R⁴ = CH₃ Ih R¹ = R² = H, R³ = R⁴ = R⁵ = CH₃ Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ and of their pharmaceutically acceptable acid addition salts, characterized in that azithromycin or its dihydrate of the formula (II) wherein IIa R¹ = H, R² = CH₃, is reacted with benzyl chloroformate in the presence of an excess of a suitable base, e.g. sodium hydrogen carbonate, in a reaction inert solvent, e.g. benzene, at a temperature of 25 °C to 60 °C, within 3 to 24 hours, followed by O-methylation of the hydroxy groups in the C-6, C-11 and C-4'' positions of the intermediate 2'-0,3'-N-bis-(benzyloxycarbonyl)-N-demethyl-erythromycin A of the formula (II), wherein IIb R¹ = R² = CO₂CH₂C₆H₅, with a 1-18 molar excess of an appropriate methylation agent, e.g. methyl iodide, dimethyl sulfate, methyl methane sulfonate or methyl p -toluenesulfonate, in the presence of an appropriate base, e.g. sodium hydride, aqueous potassium hydroxide or sodium hydroxide, in an appropriate solvent, e.g. dimethyl sulfoxide or N,N-dimethyl-formamide, or their mixtures with a reaction inert solvent, e.g. tetrahydrofurane, acetonitrile, ethyl acetate, 1,2-dimethoxyethane, at a temperature of 0 °C to room temperature, within 3 to 30 hours, yielding a mixture of O-methyl-2'-0,3'-N-bis-(benzyloxycarbonyl)-N-demethyl-azithromycin A of the formula (I), wherein Ia R¹ = R² = CO₂CH₂C₆H₅, R³ = CH₃, R⁴ = R⁵ = H Ib R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = CH₃, R⁵ = H Ic R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁵ = H, R⁴ = CH₃ Id R¹ = R² = CO₂CH₂C₆H₅, R³ = R⁴ = R⁵ = CH₃ which is optionally subjected to A) separation on a silica gel column yielding chromatographically homogenous compounds (Ia)-(1d), which are subsequently subjected to the elimination of the protecting groups in positions 2'- and 3'- by means of hydrogenolysis in a solution of lower alcohols, e.g. methanol or ethanol, in the presence of a catalyst, e.g. palladium black or palladium-on-carbon, in a hydrogen atmosphere at a pressure of 1-20 bar, under stirring of the reaction mixture, within 2-10 hours, at room temperature, yielding O-methyl-N-demethyl-azithromycin A derivatives of formula (I), wherein Ie R¹ = R² = R⁴ = R⁵ = H, R³ = CH₃ If R¹ = R² = R ⁵= H, R³ = R⁴ = CH₃ Ig R¹ = R² = R³ = R⁵ = H, R⁴ = CH₃ Ih R¹ = R² = H, R³ = R⁴ = R⁵ = CH₃ which are then subjected to reductive N-methylation of the 3'-methylamino group with 1-3 equivalents of formaldehyde (37%) and of an equal or double quantity of formic acid (98-100%) or another hydrogen source, in a reaction inert solvent chosen from halogenated hydrocarbons, e.g. chloroform, or lower alcohols, e.g. methanol or ethanol, lower ketones, e.g. acetone, at reflux temperature of the reaction mixture within 2 to 8 hours, yielding O- methylazithromycin A derivatives of formula (I), wherein Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ or B) to elimination of the protecting benzyloxycarbonyl group in 2'- and 3'-positions to hydrogenolysis, as described in A), yielding a mixture of O-methyl-azithromycin A derivatives of the formula (I), wherein Ii R¹ = R⁴ = R⁵ = H, R² = R³ = CH₃ Ij R¹ = R⁵ = H, R² = R³ = R⁴ = CH₃ Ik R¹ = R³ = R⁵ = H, R² = R⁴ = CH₃ Il R¹ = H, R² = R³ = R⁴ = R⁵ = CH₃ which is subjected to separation on a silica gel column yielding chromatographically homogenous O-methyl derivatives of azithromycin A (Ii) - (Il), which are optionally subjected to reaction with at least one equivalent of an inorganic or organic acid, yielding pharmaceutically acceptable addition salts. Use of O-methyl derivatives of azithromycin A of the formula (I), as claimed in claim 1, in the obtaining of antibacterial pharmaceutical preparations, comprising an antibacterially effective, yet physiologically acceptable amount of said compound of formula (I) and a pharmaceutically acceptable carrier.

Inventors: DJOKIC SLOBODAN, KOBREHEL GABRIJELA, LAZAREVSKI GORJANA, DJOKIC, SLOBODAN, KOBREHEL, GABRIJELA, LAZAREVSKI, GORJANA
Applicants: PLIVA PHARM & CHEM WORKS, PLIVA, FARMACEUTSKA, KEMIJSKA, PREHRAMBENA I KOZMETICKA INDUSTRIJA DIONICKO DRUSTVO
Application year: 1991
Main classification: C07H17
IPC codes: A61K31, C07D413, A61P31, C07H17