IMIDAZOLYL-ALKENOIC ACIDS

Abstract: Angiotensin II receptor antagonists having formula (I) which are useful in regulating hypertension and in the treatment of congestive heart failure, renal failure, and glaucoma, pharmaceutical compositions including these antagonists, and methods of using these compounds to produce angiotensin II receptor antagonism in mammals.

Description: TMTDAZOLYL-ALKENOIC ACIDS The present invention relates to new imidazolyl- al enoic acids which are angiotensin II receptor antagonists and are useful in regulating hypertension induced or exacerbated by angiotensin II, and in the treatment of congestive heart failure, renal failure, and glaucoma. This invention also relates to pharmaceutical compositions containing these compounds and methods for using these compounds as antagonists of angiotensin II, as antihypertensive agents and as agents for treating congestive heart failure, renal failure, and glaucoma. BACKGROUND OF THE INVEN TON The class of peptide pressor hormone known as angiotensin is responsible for a vasopressor action that is implicated in the etiology of hypertension in man. Inappropriate activity of the renin-angiotensin systems appears to be a key element in essential hypertension, congestive heart failure and in some forms of renal disease. In addition to a direct action on arteries and arterioles, angiotensin II (All), being one of the most potent endogenous vasoconstrictors known, exerts stimulation on the release of aldosterone from the adrenal cortex. Therefore, the renin-angiotensin system, by virtue of its participation in the control of renal sodium handling, plays an important role in cardiovascular hemeostasis. Interruption of the renin-angiotensin system with converting enzyme inhibitors, such as captopril, has proved to be clinically useful in the treatment of hypertension and congestive heart failure (Abrams, .B., et al., (1984), Federation Proc. f 4 , 1314). The most direct approach towards inhibition of the renin- angiotensin system would block the action of All at the receptor. Compelling evidence suggests that All also contributes to renal vasoconstriction and sodium retention that is characteristic of a number of disorders such as heart failure, cirrhosis and complications of pregnancy (Hollenberg, N.K., (1984), J. Cardiovas. Pharmacol. f £., S176) . In addition, recent animal studies suggest that inhibition of the renin-angiotensin system may be beneficial in halting or slowing the progression of chronic renal failure (Anderson, S., et al., (1985), J. Clin. Invest.- _L£, 612) . Also, a recent patent application (South African Patent Application No. 87/01,653) claims that All antagonists are useful as agents for reducing and controlling elevated intraocular pressure, especially glaucoma, in mammals. The compounds of this invention inhibit, block and antagonize the action of the hormone All, and are therefore useful in regulating and moderating angiotensin induced hypertension, congestive heart failure, renal failure and other disorders attributed to the actions of All. When compounds of this invention are administered to mammals, the elevated blood pressure due to All is reduced and other manifestations based on All intercession are minimized and controlled. Compounds of this invention are also expected to exhibit diuretic activity. Recognition of the importance of blocking and inhibiting the actions of All has stimulated other efforts to synthesize antagonists of All. The following references have disclosed imidazole derivatives which are described as having All blocking activity and useful as hypotensive agents. Furukawa et al., U.S. Patent 4,340,598 discloses imidazol-5-yl-acetic acids and imidazol-5-yl-propanoic acids. Specifically, the discloser includes l-benzyl-2- n-butyl-5-chloroimidazole-4-acetic acid and l-benzyl-2- phenyl-5-chloroimidazole-4-propanoic acid. Furukawa, et al., U.S. Patent 4,355,040 discloses substituted imidazole-5-acetic acid derivatives. A compound specifically disclosed is 1-(2-chlorobenzyl)-2- n-but l-4-chloroimidazole-5-acetic acid. Carini et al. in EP 253,310 disclose certain imidazolylpropenoic acids. Two intermediates described in this patent are ethyl 3-[1-(4-nitrobenzyl)-2-butyl-4- chloroimidazol-5-yl]propenoate and ethyl 3-[2-butyl-4- chloro-1-(4-aminobenzyl)imidazol-5-yl]propenoate. Also, Wareing, in PCT/EP 86/00297, discloses as intermediates certain imidazolylpropenoate compounds. On page 62, Formula (CX) is ethyl 3-[1(-4-fluorophenyl)-4- isopropyl-2-phenyl-lH-imidazol-5-yl]-2-propenoate. DESCRIPTION OF THE INVENTION The compounds of the present invention that are blockers of angiotensin II receptors are represented by the following Formula (I) : in which: 1 R is ada antyl, phenyl, biphenyl, or naphthyl, with each aryl group being unsubstituted or substituted by one to three substituents selected from Cl, Br, F, I, 7 C.-C^alkyl, nitro, A-CO-R , tetrazol-5-yl, C--C β alkoxy, hydroxy, S^-Cgalkyl, S0 2 NHR 7 , NHS0 2 R 7 , S0 3 H, CONR 7 R 7 , CN, S0 2 C 1 -C 8 alkyl, NHS0 2 R 7 , PO(OR 7 ) 2 , NR 7 R 7 , NR 7 COH, NR 7 COC 1 -C 6 alkyl, NR 7 CON(R 7 ) 2 , NR 7 COW, W, S0 2 W; m is 0 or 2-4; 2 R is C 2 -C 10 alkyl, C 3 -C 10 alkenyl, C 3 -C 10 alkynyl, C- j -C 8 Cycloalkyl, or (CH Q _ 8 phenyl unsubstituted or substituted by one to three substituents selected from C 1 -C β alkyl, nitro, Cl, Br, F, I, hydroxy, C.-C 8 alkoxy, NR 7 R , C0 2 R 7 , CN, CONR 7 R 7 , W, tetrazol-5-yl, NR 7 COC 1 -C 8 alkyl, NR 7 COW, SC^Cgalk l, S0 2 , or S0 2 C 1 -Cgalkyl; 7 X is a single bond, S, NR , or 0; 3 R is hydrogen, Cl, Br, F, I, CHO, hydroxymethyl, COOR 7 , CONR 7 R 7 , NO,, W, CN, NR 7 R 7 , or phenyl; R 4 and R5 are independently hydrogen, C,-C^alkyl, or phenyl-Y-, naphthyl-Y-, or biphenyl-Y-, wherein the aryl groups are unsubstituted or substituted by one to three substituents selected from Cl, Br, F, I, C -Cgalkoxy, hydroxy, CO2 7 , CN, NO2, tetrazol-5-yl, SO3H, CF3, CONR 7 R 7 , S0 2 NHR 7 , Cx-Cgalkyl, or NR 7 , R 7 ' * or by methylenedioxy, phenoxy or phenyl, thienyl-Y-, furyl-Y-, pyrazolyl-Y-, imidazolyl-Y-, pyrrolyl-Y-, triazolyl-Y-, oxazolyl-Y-, isoxazolyl-Y-, thiazolyl-Y-, pyridyl-Y-, or the proviso that R 4 and R**- * are not both selected from hydrogen; Y is a single bond, 0, S, or C,-C 6 alkyl which is straight or branched or optionally substituted by phenyl or benzyl, wherein each of the aryl groups is N0 2 , CF,, C 1 ~ Z is a single bond, vinyl, -CH 2 -0-CH ? -, methylene optionally substituted by C..-C h alky1, one or two benzyl groups, thienylmethyl, or fur lmethyl, or -C(0)NHCHR -, 9 wherein R is H, C,-C 8 alkyl, phenyl, benzyl, thienylmethyl, or furylmethyl; W is CnF2~n+ ,,l , CnF2n+.l. , wherein n is 1-3; A is -(CH 2 ) m -, -CH=CH-, -0(CH 2 ) n -, or -S(CH 2 ) n ~; each R independently is hydrogen, C.,-Cgalkyl, or (CH2)mp p heny J l, wherein m is 0-4; and R is hydrogen, C.,-Cgalkyl, or 2-di (C.,-Cgalkyl)- amino-2-oxoethyl; or a pharmaceutically acceptable salt thereof. Preferably, one of R 4 and R5 i.s hydrogen or C.,- Cgalkyl. Preferred compounds of this invention are represented by Formula (I) when: 1 R is phenyl unsubstituted or substituted by one to three substituents selected from chloro, fluoro, trifluoromethyl, nitro, methyl, methoxy, hydroxy, sulfamyl, carboxy, carboC.-Cgalkoxy, carbamoyl, cyano, or tetrazol-5-yl; m is 0 or 2; X is a single bond or S; R 2 i.s C 2 -C 8 alkyl; R is hydrogen, chloro, fluoro, or trifluoromethyl; 4 , R is hydrogen or C 1 -C 8 alkyl; R is thienylmethyl, furylmethyl, imidazolylmethyl, or pyridylmethyl, each of which is optionally substituted by methyl or methoxy; or benzyl unsubstituted or substituted by one to three substituents selected from Cl, Br, F, NO2, OCH3, OH, CF3, NR 7 R 7 , or methylenedioxy; R is COOH, COOC._ 2 alkyl, or CONH 2 ; and each R 7 independently is H or CH3; or a pharmaceutically acceptable salt thereof. The E isomers (trans stereochemistry of the carboxy and imidazole groups) are generally more active and thus, are preferred over the Z isomers (cis) . As used herein, the terms alkyl, alkenyl, alkoxy and alkynyl mean carbon chains which are branched or unbranched with the length of the chain determined by the descriptor preceding the term. Particular compounds of the invention include, but are not limited to, the following: (E)-3-[2-n-butyl-l-{4-carboxyphenyl}-lH-imidazo-5- yl]-2-(2-thienyl)methyl-2-propenoic acid and (E)-3-[2-n-butyl-l-{ (4-carboxyphenyl)ethyl}-lH- imidazo-5-yl]-2-(2-thienyl)methyl-2-propenoic acid; or a pharmaceutically acceptable salt thereof. The invention also relates to pharmaceutical compositions comprising a pharmaceutical carrier and an effective amount of a compound of Formula (I) . Also included in the present invention are methods for antagonizing angiotensin II receptors which comprises administering to a subject in need thereof an effective amount of a compound of Formula (I) . Methods of producing antihypertensive activity and methods of treating congestive heart failure, glaucoma, and renal failure by administering these compounds are also included in this invention. The compounds of this invention are prepared by procedures described herein and illustrated by the examples. Reagents, protecting groups and functionality on the imidazole and other fragments of the molecule must be consistent with the proposed chemical transformations. Steps in the synthesis must be compatible with the functional groups and the protecting groups on the imidazole and other parts of the molecule. 2 The starting materials, 2-R X-imidazole, are known to the art (J. Org. Chem. 45:4038, 1980) or are synthesized by known procedures. For example, imidazole is converted to 2-n-butylimidazole by reacting imidazole with triethylorthoformate and p-toluenesulfonic acid to give 1-diethoxyorthoamide imidazole and then treating with n-butyl lithium to give the 2-lithium derivative of the orthoamide and alkylating with n-butyl iodide in a suitable solvent, such as tetrahydrof ran (THF) . The following procedure is useful for the preparation of compounds of Formula (I) particularly where R 1 is 2-chlorophenyl or 4-carboxyphenyl, R2 is n- butyl or n-propyl, X is a single bond or S, R3 is hydrogen, chloro, or CF,, R 4 is hydrogen, R5 is as 6 o o described in Formula (I) , R is COOR and R is hydrogen, methyl, or ethyl. The 1-R 1 (CH 2 ) -group is incorporated onto the 2-R2X- imidazole by known procedures, for example, by reaction 1 with an R -CH ? halide, mesylate or acetate, such as 2- chlorobenzyl bromide, in a suitable solvent, such as dimethylformamide (DMF) , in the presence of a suitable acid acceptor, such as sodium alkylate, potassium or sodium carbonate, or a metal hydride, preferably sodium hydride at a reaction temperature of 25°C to 100°C, preferably 50°C. The resulting 1-R 1 (CH 2 ) -2-R2X imidazole is hydroxymethylated in the 5-position, for example, by reacting with formaldehyde in the presence of sodium acetate in acetic acid to provide the 1-R 1CH 2 ~2-R2X-5-hydroxymethyl- imidazole intermediates. 1 2 Alternatively, the 1-R (CH 2 ) m -2-R -5-hydroxymethyl- imidazole intermediates are prepared by reacting an imido 2 ether, R -C(=NH)-O-alkyl, such as valeramidme methyl ether, with dihydroxyacetone in liquid ammonia under 2 pressure to give 2-R -5-hydroxymethylimιdazole. This intermediate is reacted with acetic anhydride to give 1- 2 acetyl-5-acetoxymethyl-2-R -imidazole. The diacetate intermediate is N-alkylated, for example, using 2- chlorobenzyl triflate and the resulting 1-R 1 (CH 2 ) -2-R2- 5-acetoxy-methylimidazole is treated with aqueous base, such as 10% sodium hydroxide solution, to give the 1- R 1 (CH Δt)IR-2-R2-5-hydroxymethyηl-ι.mι.dazole intermediate. Alternatively, the 2-R S-imidazole compounds are prepared by the following procedure. Benzylamines, substituted by one to three substituents selected from halo, C- galkyl, CN, N0 2 , CF 3 , C0 2 C., galkyl, SC 1 _ g alkyl, or S0 2 C 1 _ g alkyl, are alkylated with a C 1 _ g alkyl chloroacetate, for example methyl chloroacetate, in the presence of a base, such as triethylamine, in a suitable solvent, such as dimethylformamide. The — o — resulting alkylaminoalkyl ester compounds are N- formulated with formic acid in the presence, of a suitable solvent, such as xylenes, followed by C-formulation of the carbon alpha to both the amino and the ester groups. Reaction of this intermediate with acidic thiocyanate, preferably potassium thiocyante, in an inert organic solvent, such as a C, «alkyl alcohol, produces 1-RCH 2 ~2- mercapto-5-alkanoate ester imidazole compounds. The free thio group of the ester imidazole is reacted with a halo- R compound, wherein R is C 2 _ 1Q alkyl, C 3 _ 10 alkenyl, C 3 -C 1Q alkynyl, C 3 -CgCycloalkyl or an optionally substituted (CH 2 ) 0 _ g phenyl, preferably propyl bromide, in the presence of a suitable base, such as sodium carbonate, in an appropriate solvent, such as ethyl acetate. The ester is reduced to the hydroxymethyl- imidazole intermediate by reduction with a suitable reagent, preferably diisobutyl aluminum hydride, in an appropriate solvent, such as tetrahydrofuran, at a temperature of -78°C to 25°C, preferably at less than -10°C. The hydroxymethyl group of the hereinbefore prepared intermediate is oxidized to an aldehyde by treatment with a suitable reagent, such as anhydrous chromic acid-silica gel in tetrahydrofuran or, preferably, with activated manganese dioxide, in a suitable solvent, such as benzene or toluene, or preferably methylene chloride, at a temperature of 25°C to 140°C, preferably at 25°C. The 1- R 1(CH ) -2-R2X-ιmιdazol-5-carboxaldehydes are reacted with an appropriate phosphonate, such as those listed in Table I (Examples 2-5) . The phosphonates are prepared, for example, from trialkyl phosphonoacetates by alkylation with an appropriate halide, mesylate or acetate in the presence of a suitable base, such as sodium hydride, in a suitable solvent, preferably glyme at a reaction temperature of 25°C to 110°C, preferably at 55°C, to provide, for example, the phosphonates listed in Table I. The reaction of the imidazol-5-carboxaldehydes with the phosphonates is performed in the presence of a suitable base, such as a metal alkoxide, lithium hydride or preferably sodium hydride, in a suitable solvent, such as ethanol, methanol, ether, dioxane, tetrahydrofuran, or preferably glyme, at a reaction temperature of 10°C to 50°C, preferably at 25°C, to provide a variable mixture of trans and cis, e.g., (E) and (Z) , 1-R 1 (CH,) -2-R 2 X-5- 5 CH=C(R )- (COOalkyl)-imidazoles. These isomers are readily separated by chromatography over silica gel in suitable solvent systems, preferably hexane in ethyl acetate mixtures. The esters are hydrolyzed to the acids, l-R 1 -(CH 2 )- n -2-R 2 X-5-CH=C(R 5 )C00H-imidazoles, using bases, such as potassium hydroxide, lithium hydroxide or sodium hydroxide, in a suitable solvent system, such as, for example, aqueous alcohols or diglyme. The trans and cis structures of the acids are readily determined by NMR by the NOE protocol, as well as by the biological activities since, generally, the trans (E) isomeric acids are the more potent isomers. Alternatively, the 1-R 1 (CH 2 ) m -2-R 2 X-imidazol-5- carboxaldehydes are prepared by the following procedure. Starting 2-R 2X-ιmιdazol-5-carboxaldehydes are reacted with an N-alkylating protecting reagent, such as chloromethyl pivalate (POM-C1) , in the presence of a base, such as potassium carbonate, in a suitable solvent, such as dimethylformamide, at a temperature of 20°C to 50°C, preferably at 25°C, to give N-alkylation (e.g., POM-derivation) on the least hindered nitrogen atom of ι the imidazole nucleus. The 1-R (CH 2)m-group is incorporated onto the imidazole by N-alkylation of the above prepared aldehyde with a halomethylbenzene compounds, such as methyl 4-bromomethyl-3-chlorobenzoate, at a temperature of 80°C to 125°C, preferably at 100°C. The protecting group on the 3-nitrogen of the imidazole ring is removed by base hydrolysis, for example using a biphasic mixture of ethyl acetate and aqueous sodium carbonate, to give 1-R 1CH 2 ~2-R2X-imιdazole-5- carboxaldehyde compounds. The Formula (I) compounds can be prepared from these 5-carboxaldehyde compounds by the methods described above. g 8 1 Compounds of Formula (I), wherein R is COOR , R , R ? , R3, R4 and R5 are as described in Formula (I) , and R8 is H, methyl or ethyl, are also prepared by the following procedure. 2 The 2-R X-imidazole starting materials are reacted with trimethylsilylethoxymethyl(SEM) chloride to give 1- 2 (trimethylsilyl)ethoxymethyl-2-R X-imidazole. The reaction is carried out, for example, in the presence of sodium hydride in a solvent such as dimethylformamide. The 5-tributyltin derivatives are prepared by lithiation with, for example, butyllithium in a suitable solvent, preferably diethyl ether, followed by treatment of the lithio imidazole derivative with a tributyltin halide, preferably tri-N-butyltin chloride, at -10°C to 35°C, preferably at 25°C. The l-SEM-2-R 2 X-5- tributyltinimidazole is coupled with an α,β-unsaturated acid ester having a leaving group on the β-position, such as a halide or trifluoromethanesulfonyloxy group, for example, BrCR 4=C(R5) ( ' COOalkyl) , in the presence of a phosphine ligand, such as bis(diphenyl-phosphino)propane, or triphenylphosphine and a palladium (II) compound, or preferably tetrakis (triphenylphosphine)-palladium(0) , with or without a base, such as tributylamine, at a temperature of 50°C to 150°C, preferably at 120°C. Both the (E) and (Z) olefinic isomers are prepared by this procedure, and the isomeric esters are readily separated by chromatography over silica gel. The 1-SEM group from the (E) and (Z) isomers is hydrolyzed with acid, for example, aqueous hydrochloric, in a suitable alcoholic solvent, such as methanol or ethanol, and the 1- unsubstituted imidazole derivatives are converted to the 1-t-butoxycarbonyl (t-BOC) imidazoles with di-t-butyl dicarbonate (Hoppe-Seyler's Z. Physiol. Chem., (1976), 357 f 1651) . The t-BOC esters are alkylated and hydrolyzed with, for example, 2-chlorobenzyl-O-triflate in the presence of a suitable base, preferably diisopropylethylamine, in a suitable solvent, preferably ethylene chloride, to afford the l-(2- chlorophenyl)methyl-imidazole derivatives (esters) . The (E) and (Z) isomers are hydrolyzed to the (E) and (Z) acids by the method described above. Compounds of Formula (I) are also prepared by the following procedure. The 1-R 1 (CH -2-R2X-imidazole-5- carboxaldehydes, prepared as described above, are reacted with a substituted half-acid, half-ester derivative of a malonate, such as ethyl 2-carboxy-3-(2- thienyDpropionate, in the presence of a base, such as piperidine, in a suitable solvent, such as toluene, at a temperature of 80°C to 110°C, preferably at 100°C. The resulting 1-R 1 (CH 2 ) m -2-R 2 X-5-CH=C(R 5 )COOalkylimidazoles are hydrolyzed to the corresponding Formula (I) acid compounds by alkaline hydrolysis as described above. 1 Compounds of Formula (I) in which R is 2- 2 chlorophenyl or 4-carboxyphenyl, R is n-butyl or n- propyl, X is- a single bond or S, R 3 is H, Cl, or CF-,, R4 c __ R is methyl, R is as described in Formula (I) , R is COOR and other parameters are as described above are prepared as follows. The 1-R 1 (CH 2 ) m -2-R 2 X-imidazol-5- carboxaldehydes, prepared as described above, are converted to the corresponding alcohols with an organo- metallic derivative or Grignard reagent, preferably methyl lithium, in a suitable solvent, such as tetrahydrofuran. The alcohol is oxidized, for example, using manganese dioxide to give the ketone. The olefinic esters are prepared from the ketone by reaction with appropriate phosphonates to give the (E) and/or (Z) isomers which are readily separated. The acids are prepared from the esters by alkaline hydrolysis as described above. Compounds of Formula (I) in which R 3 is H, Cl, 1 CH 2 OH, or CF 3 are prepared as follows. The 1-R -(CH 2 ) - 2-R X-imidazol-5-carboxaldehydes are treated with the lithium derivative of a substituted ethyl or methyl ester. These lithio derivatives are prepared from the reaction of lithium diisopropylamide in a suitable solvent, preferably tetrahydrofuran, with an acid ester, such as R00C-CH 2 -Y-(2-thienyl) , to generate the a-lithio derivatives at -78°C to -10°C, preferably at -78°C, which are then treated with the imidazol-carboxaldehyde. The intermediate β-hydroxy group of the imidazole ester is converted to a mesylate or an acetate and the mesylate, or preferably the acetate, is heated in a suitable solvent, such as toluene, with one to two equivalents of l,8-diazo-bic clo[5.4.0]undec-7-ene, at 50 to 110°C, preferably at 80°C, to afford ester compounds of Formula (I) such as 3-(imidazol-5-yl)-2-(2-thienyl)methyl-2- propenoic acid esters. The (E) isomer is the predominate olefinic isomer. The acids are prepared from the esters by the method described above. 1 Compounds of Formula (I) , wherein R is 2- chlorophenyl or 4-carboxyphenyl, R 2 is n-butyl or n- 3 propyl, X is a single bond or S, R is H, Cl, CF,,, or CH 0H, R 4 is H, R5 is heterocycli.c or a substituted heterocyclic group as described in Formula (I) and R 6 is COOH, may be prepared " by heating 1-R 1 -(CH 2 ) m -2-R 2 X- imidazol-5-carboxaldehydes at 50°C to 180°C, preferably at 140°C, with an appropriate substituted heterocyclic acetic acid and with acetic anhydride and potassium carbonate to provide unsaturated acids of Formula (I) , such as 3-[2-n-butyl-l-(2-chlorophenyl)methyl-lH- 5 imidazol-5-yl]-2-R -2-propenoιc acid. The trans olefinic acid is the principal product. 6 8 Compounds of Formula (I) in which R is Z-COOR where Z is an optionally substituted methylene group are prepared by reducing the trans or (E) isomers of 3- (imidazol-5-yl)-2-propenoic acid esters (prepared as described above) with an appropriate hydride reagent, preferably diisobutylaluminum hydride, in a suitable solvent, such as tetrahydrofuran, to provide the unsaturated alcohol compounds. These compounds are reacted with ethyl chlor.oformate, for example, with a base, preferably triethylamine, in a suitable solvent, such as tetrahydrofuran, to give 5-EtOOCOCH 2 CR 5=CR4- imidazoles which are reacted with carbon monoxide in the presence of a phosphine ligand, preferably triphenyl¬ phosphine with palladium (II) acetate, in a suitable solvent, preferably tetrahydrofuran, at a temperature of 25°C to 100°C, preferably at 40°C, to give the 5- EtOOCCH 2 CR 5=CR4-imidazoles. The corresponding acids are prepared from these ethyl esters by base hydrolysis as described above. p Compounds of Formula (I) in which Z is -CH 2 C00R having additional substitution on the carbon a to the the ester with a lithium dialkylamide, preferably lithium diisopropylamide, and then treating with an alkylating agent, such as methyl halide, benzyl bromide, or heterocyclic methyl halide, to provide the mono-alkylated product compounds or the dialkylated product compounds. The acid compounds are prepared from the esters by base hydrolysis. 6 8 Compounds of Formula (I) in which R is Z-COOR where Z is -CH 2 -0-CH 2 - are prepared from unsaturated alcohol compounds, which had been obtained by the reduction of the Formula (I) propenoic acid esters. The alcohol is reacted with an appropriate hydride reagent, such as sodium hydride, in a suitable solvent, such as glyme, followed by reaction with an alkylating reagent, such as methyl bromoacetate, to give the 5-Me00CCH 9 -0- The corresponding acids are prepared from these esters by base hydrolysis as described above. c o Compounds of Formula (I) in which R is Z-COOR 9 where Z is -C(0)NHCHR - are prepared from the Formula (I) propenoic acid compounds. These acids are reacted with an appropriately substituted amino acid, such as glycine methyl ester hydrochloride or phenylalanine methyl ester hydrochloride, in the presence of an amide-forming reagent, such as N-hydroxysuccinimide and dicyclohexylcarbodiimide, in the presence of a base, for example triethylamine, in a suitable solvent, such as tetrahydrofuran, at a temperature of 20°C to 50°C, preferably at 35°C. The 5-C 1 _ 4 alkyl-OOCCHR 9 NHC(0)- CH 2 CR =CR -imidazoles are converted to their corresponding acids by base hydrolysis as described above. Compounds of Formula (I) in which the R substituent is substituted by hydroxy are formed from Formula (I) compounds in which the R group is substituted by C j -C.alkoxy using an ether-cleaving reagent, such as boron tribromide or hydrobromic acid. Compounds of Formula (I) in which the R 1 substituent is substituted by carboxy are formed from Formula (I) compounds in which the R group is substituted by CO C,-C.alkyl using basic hydrolysis, such as aqueous sodium or potassium hydroxide in methanol or ethanol, or using acidic hydrolysis, such as aqueous hydrochloric acid. Compounds of Formula (I) in which the R substituent is substituted by a tetrazol-5-yl group are prepared from the correponding carboxy compounds. For example, Formula (I) acid compounds are reacted with a halogenating agent, such as thionyl chloride, in a suitable solvent, for example benzene, to give the corresponding acid halide compounds. The acid halides are then converted to primary amide compounds in a reaction with concentrated ammonia. Subsequent dehydration of the amides with oxalyl chloride/dimethylformamide in acetonitrile/dimethylformamide yields the nitrile compounds, which are the immediate precursors to the Formula (I) tetrazole compounds. Tetrazole formation is accomplished by reacting the nitriles with azide, preferably aluminum azide prepared in situ by the reaction of sodium azide with aluminum chloride, in a suitable solvent, for example tetrahydro ran. The . Formula (I) compounds in which R is -Z-C0 2 H are prepared from these Formula (I) tetrazole ester compounds by basic hydrolysis as described above. Pharmaceutically acceptable acid addition salts of compounds of Formula (I) are formed with appropriate organic or inorganic acids by methods known in the art. For example, the base is reacted with a suitable inorganic or organic acid in an aqueous miscible solvent such as ethanol with isolation of the salt by removing the solvent or in an aqueous immiscible solvent when the acid is soluble therein, such as ethyl ether or chloroform, with the desired salt separating directly or isolated by removing the solvent. Representative examples of suitable acids are maleic, fumaric, benzoic, ascorbic, pamoic, succinic, bismethylenesalicylic, methanesulfonic, ethanedisulfonic, acetic, propionic, tartaric, salicylic, citric, gluconic, aspartic, stearic, palmitic, itaconic, glycolic, p-aminobenzoic, glutamic, benzenesulfonic, hydrochloric, hydrobromic, sulfuric, cyclohexylsulfamic, phosphoric and nitric acids. Pharmaceutically acceptable base addition salts of p compounds of Formula (I) in which R is H are prepared by known methods from organic and inorganic bases, including nontoxic alkali metal and alkaline earth bases, for example, calcium, lithium, sodium, and potassium hydroxide; ammonium hydroxide, and nontoxic organic bases, such as triethylamine, butylamine, piperazine, meglumine, choline, diethanolamine, and tromethamine. Angiotensin II antagonist activity of the compounds of Formula (I) is assessed by in vitro and in vivo methods. in vitro antagonist activity is determined by the ability of the compounds to compete with 125I- angiotensin II for binding to vascular angiotensin II receptors and by their ability to antagonize the contractile response to angiotensin II in the isolated rabbit aorta. In. vivo activity is evaluated by the efficacy of the compounds to inhibit the pressor response to exogenous angiotensin II in conscious rats and to lower blood pressure in a rat model of renin dependent hypertension. Binding The radioligand binding assay is a modification of a method previously described in detail (Gunther et al., Circ. Res. _47_:278, 1980) . A particular fraction from rat mesenteric arteries is incubated in Tris buffer with 80 pM of 125I-angι.otensm. II wi.th or wi.thout angi,otensin II antagonists for 1 hour at 25°C. The incubation is terminated by rapid filtration and receptor bound 125I- angiotensin II trapped on the filter is quantitated with a gamma counter. The potency of angiotensin II antagonists is expressed as the IC ι - n which is the concentration of antagonist needed to displace 50% of the total specifically bound angiotensin II. The IC50 of (E)-3-[2-n-but l-l-{4-carboxyphenyl}-lH-imidazo-5-yl]-2- (2-thienyl) methyl-2-propenoic acid is about 250 .0 nM. Aorta. The ability of the compounds to antagonize angiotensin II induced vasoconstriction is examined in the rabbit aorta. Ring segments are cut from the rabbit thoracic aorta and suspended in organ baths containing physiological salt solution. The ring segments are mounted over metal supports and attached to force displacement transducers which are connected to a recorder. Cumulative concentration response curves to angiotensin II are performed in the absence of antagonist or following a 30-minute incubation with antagonist. Antagonist disassociation constants (K-J are calculated by the dose ratio method using the mean effective concentrations. The K B of (E)-3-[2-n-butyl-l-{4- carboxyphenyl}-lH-imidazo-5-yl]-2-(2-thienyl)methyl-2- propenoic acid is about 25 nM. Inhibition of pressor response to angiotensin IT in conscious rats Rats are prepared with indwelling femoral arterial and venous catheters and a stomach tube (Gellai et al., Kidney In . 15:419, 1979) . Two to three days following surgery the rats are placed in a restrainer and blood pressure is continuously monitored from the arterial catheter with a pressure transducer and recorded on a polygraph. The change in mean arterial pressure in response to intravenous injections of 250 mg/kg angiotensin II is compared at various time points prior to and following the administration of the compounds intravenously or orally at doses of 0.1 to 300 mg/kg. The dose of compound needed to produce 50% inhibition of the control response to angiotensin II (IC-- 0 ) is used to estimate the potency of the compounds. Ant ■ ..hypertensive activity The antihypertensive activity of the compounds is measured by their ability to reduce mean arterial pressure in conscious rats made renin-dependent hypertensive by ligation of the left renal artery (Cangiano et al., J. Pharmacol. Exp. Ther. 211-1:310, 1979) . Renal artery ligated rats are prepared with indwelling catheters as described above. Seven to eight days following renal artery ligation, the time at which plasma renin levels are highest, the conscious rats are placed in restrainers and mean arterial pressure is continuously recorded prior to and following the administration of the compounds intravenously or orally. The dose of compound needed to reduce mean arterial pressure by 30 mm Hg (1C 3Q ) is used as an estimate of potency. The intraocular pressure lowering effects employed in this invention may be measured by the procedure described by Watkins, et al., J. Ocular Pharmacol. f 1 (2) :161-168 (1985) . The compounds of Formula (I) are incorporated into convenient dosage forms, such as injectable preparations, or for orally active compounds, capsules or tablets. •Solid or liquid pharmaceutical carriers are employed. Solid carriers include starch, lactose, calcium sulfate dihydrate, terra alba, sucrose, talc, gelatin, agar. pectin, acacia, magnesium stearate, and stearic acid. Liquid carriers include syrup, peanut oil, olive oil, saline, and water. Similarly, the carrier or diluent may include any prolonged release material, such as glyceryl monostearate or glyceryl distearate, alone or with a wax. The amount of solid carrier varies widely but, preferably, will be from about 25 mg to about 1 g per dosage unit. When a liquid carrier is used, the preparation will be in the form of a syrup, elixir, emulsion / soft gelatin capsule, sterile injectable liquid, such as an ampoule, or an aqueous or nonaqueous liquid suspension. For topical ophthalmolgic administration, the pharmaceutical compositions adapted include solutions, suspensions, ointments, and solid inserts. Typical pharmaceutically acceptable carriers are, for example, water, mixtures of water and water-miscible solvents such as lower alkanols or vegetable oils, and water soluble ophthalmologically acceptable non-toxic polymers, for example, cellulose derivatives such as methyl cellulose. The pharmaceutical preparation may also contain non-toxic auxiliary substances such as emulsifying, preserving, wetting, and bodying agents, as for example, polyethylene glycols; antibacterial components, such as quarternary ammonium compounds; buffering ingredients, such as alkali metal chloride; antioxidants, such as sodium metabisulfite; and other conventional ingredients, such as sorbitan monolaurate. Additionally, suitable ophthalmic vehicles may be used as carrier media for the present purpose including conventional phosphate buffer vehicle systems. The pharmaceutical preparation may also be in the form of a solid insert. For example, one may use a solid water soluble polymer as the carrier for the medicament. Solid water insoluble inserts, such as those prepared from ethylene vinyl acetate copolymer, may also be utilized. The pharmaceutical preparations are made following conventional techniques of a pharmaceutical chemist involving mixing, granulating, and compressing, when necessary, for tablet forms, or mixing, filling and dissolving the ingredients, as appropriate, to give the desired oral, parenteral, or topical products. Doses of the compounds of Formula (I) in a pharmaceutical dosage unit as described above will be an efficacious, nontoxic quantity selected from the range of .01 - 200 mg/kg of active compound, preferably 1 - 100 mg/kg. The selected dose is administered to a human patient in need of angiotensin II receptor antagonism from 1-6 times daily, orally, rectally, topically, by injection, or continuously by infusion. Oral dosage units for human administration preferably contain from 1 to 500 mg of active compound. Preferably, lower dosages are used for parenteral administration. Oral administration, at higher dosages, however, also can be used when safe and convenient for the patient. Topical formulations contain the active compound in an amount selected from 0.0001 to 0.1 (w/v%) , preferably from 0.0001 to 0.01. As a topical dosage unit form, an amount of active compound from between 50 ng to 0.05 mg, preferably 50 ng to 5 mg, is applied to the human eye. The method of this invention of antagonizing angiotensin II receptors in mammals, including humans, comprises administering to a subject in need of such antagonism an effective amount of a compound of Formula (I) . The method of this invention of producing antihypertensive activity and the method of treating congestive heart failure, glaucoma, and renal failure comprise administering a compound of Formula (I) to a subject in need thereof an effective amount to produce said activity. Contemplated equivalents of Formula (I) compounds are compounds otherwise corresponding thereto wherein substituents have been added to any of the unsubstituted positions of the Formula (I) compounds provided such compounds have the pharmaceutical utility of Formula (I) compounds. The following examples illustrate preparation of compounds and pharmaceutical compositions of this invention. The examples are not intended to limit the scope of this invention as defined hereinabove and as claimed below. Example 1 fF,.-3-r2-n-Bπ yl-l-(4-carboxvphenvl -lH-imiriazo-5-yl1-2- f?- hienγl.methvl-2-propenoic Acid Imidazole was converted to the 1-diethoxyorthoamide derivative by the method of Curtis and Brown, J. Qrσ. Chem. r (1980), 4ϋ, 20. Imidazole (12.8 g, 0.19 mol) and 118.4 g (0.8 mol) of triethylorthoformate were reacted in the presence of 1 g of p-toluenesulfonic acid to give 20.6 (61%), bp 65-70°C (0.1 mm) of 1-diethoxyorthoamide imidazole. This product (24.0 g, 0.14 mol) was dissolved in dry tetrahydrofuran (250 mL) , cooled to -40°C and n- butyl lithium (0.14 mol, 56.4 mL of 2.5 M in hexane) was added at -40°C to -35°C. After 15 minutes n-butyl iodide (31.1 g, 0.169 mol) was added at -40°C, and the reaction was stirred overnight at ambient temperature. The reaction was partitioned between ether and 0.3 N hydrochloric acid, and the organic layer was repeatedly extracted with dilute hydrochloric acid. The combined aqueous extracts were neutralized with sodium bicarbonate solution, extracted with methylene chloride, dried over magnesium sulfate and concentrated. A flash distillation on a Kugelrohr apparatus provided 14.8 g (85%) of 2-n- butylimidazole. A mixture of 2-n-butylimidazole (12.42 g, 0.10 mol), ethyl 4-fluorobenzoate (19.0 g, 0.113 mol) and potassium carbonate (27.64 g, 0.20 mol) in 100 mL of dimethylsulfoxide was stirred at 135°C under argon for 16 hours. The mixture was cooled, diluted with diethyl ether and filtered. The filter cake was washed thoroughly with diethyl ether. The combined organic extracts were washed with water and brine and then dried with sodium sulfate. The solvent was removed in vacuo to give 16.10 g of crude product. The product was purified by flash chromatography on silica gel eluting with hexane/ethyl acetate (80/20 to 60/40) to give 7.82 g (29%) of 2-n-butyl-l- (4-carboethoxyphenyl)-lH-imidazole. A mixture of 2-n-butyl-l-(4-carboethoxyphenyl)-1H- imidazole (3.39 g, 0.0124 mol), 37% formaldehyde (11.5 mL, 0.153 mol), sodium acetate (1.82 g, 0.0222 mol) and acetic acid (1.36 mL) was heated to reflux for 40 hours under argon. The reaction was concentrated in vacuo, and the residue was stirred with 500 L of 20% sodium hydroxide solution for 4 hours, diluted with water and extracted with methylene chloride. The extract was washed, dried, and concentrated. The crude product was flash chromatographed over silica gel with a gradient of ethyl acetate to 10% of methanol in ethyl acetate to give 0.81 g of 2-n-butyl-l-(4-carboethoxyphenyl)-5- hydroxy ethyl-lH-imidazole. To a solution of 2-n-butyl-l-(4-carboethoxyphenyl)- 5-hydroxymethyl-lH-imidazole (0.78 g, 0.0026 mol) in methylene chloride (40 mL) was added activated manganese dioxide (2.4 g) . The suspension was stirred at room " temperature for 18 hours.* The solids were filtered and the filtrate concentrated 0.70 g (91%) of 2-n-butyl-l-(4- carboethoxyphenyl)-lH-imidazol-5-carboxaldehyde, as an oil. Ethyl 2-carboxy-3- (2-thienyl)propionate (14 g, 0.061 mol) was prepared by stirring a solution of diethyl 2- thienylmalonate (16.8 g, 0.0655 mol) and potassium hydroxide (4.41 g, 0.0786 mol) in 200 ml of ethanol under argon at room temperature for 12 days and then purifying by removing the solvent under vacuum, dissolving the reside in water, washing the aqueous layer with aqueous hydrochloric acid and with diethyl ether. A mixture of the half-acid, half-ester, prepared above, (4.64 g, 0.0203 mol), 2-n-butyl- (4- carboxyethoxyphenyl)imidazol-5-aldehyde (0.66 g, 0.0022 mol), piperidine (0.56 g, 65 mL, 0.0066 mol), and 3.07 mL of pyridine in 75 mL of benzene was refluxed using a Dean-Stark trap to remove the water. The mixture was refluxed for 2.5 hours. The mixture was concentrated in vacuo, removing traces of pyridine by azeotroping with toluene. The crude product was flash chromatographed on silica gel eluting with hexane/ethyl acetate and then this product was rechromotographed eluting with chloroform/acetone (97.5/2.5) to give 0.79 g of ethyl (E) -3-[2-n-butyl-l-(4-carboxyethoxyphenyl)-lH-imidazol-5- yl]-2-(2-thienyl)methyl-2-propenoate. A mixture of ethyl (E)-3-[2-n-butyl-l- (4-carbo¬ ethoxyphenyl)-lH-imidazol-5-yl]-2-(2-thienyl)methy1-2- propenoate and sodium hydroxide (0.40 g, 0.01 mol) in 2:1 ethanol/water (15 mL) was stirred at room temperature for 18 hours. The mixture was filtered, diluted with 15 mL of water, chilled in an ice bath and then the pH was adjusted to 4.0 with 10% aqueous hydrochloric acid solution. The resulting solid was collected, washed with water and dried to give 0.633 g (95%) of the title compound; mp 260-256°C(d) . Example 2 ■E.-3-r2-n-Butyl-l-f (4-carboxvphenγl. ethyl . -lH-imidazo-5- yll-2- (2-thienyl)methyl-2-propenoic Acid The title compound was prepared following the procedure of Example 1; mp 256-259°C(d) . Example 3 An oral dosage form for administering orally active Formula (I) compounds is produced by screening, mixing and filling into hard gelatin capsules the ingredients in proportions, for example, as shown below. Ingredients Amounts (E)-3-[2-n-butyl-l-{ (4-carboxyphenyl)- ethyl}-lH-imidazol-5-yl]-2-(2- thienyl)methyl-2-propenoic acid 100 mg magnesium stearate 10 mg lactose 100 mg Example 4 The sucrose calcium sulfate dihydrate and orally active Formula (I) compounds are mixed and granulated with a 10% gelatin solution. The wet granules are screened, dried, mixed with the starch, talc and stearic acid, screened and compressed into a tablet. Ingredients Amounts (E)-3-[2-n-butyl-l-{4-carboxyphenyl}- lH-imidazol-5-yl]-2-(2-thienyl)methyl- 2-propenoic acid calcium sulfate dihydrat sucrose starch talc stearic acid (E)-3-[2-n-butyl-l-{ (4-carboxyphenyl)ethyl}-1H- imidazol-5-yl]-2- (2-thienyl)methyl-2-propenoic acid, 50 mg, is dispersed in 25 ml of normal saline to prepare an injectable preparation. Example 6 A topical opthamological solution for administering Formula (I) compounds is produced by mixing under sterile conditions the ingredients in proportions, for example, as shown below. Ingredients (E)-3-[2-n-butyl-l-{4-carboxyphenyl}- lH-imidazol-5-yl]-2-(2-thienyl)methyl- 2-propenoic acid dibasic sodium phosphate monobasic sodium phosphate chlorόbutanol hydroxypropanol methylcellulose sterile water 1.0 N sodium hydroxide It is to be understood that the invention is not limited to the embodiments illustrated hereabove and the right to the illustrated embodiments and all modifications coming within the scope of the following claims is reserved.

Claims: What is claimed is : 1. A compound of the formula: (I) in which: R is adamantyl, phenyl, biphenyl, or naphthyl, with each aryl group being unsubstituted or substituted by one to three substituents selected from Cl, Br, F, I, 7 C 1 -C 8 alkyl, nitro, A-C0 2 R , tetrazol-5-yl, C.-Cgalkoxy, hydroxy, S0 2 NHR 7 , NHS0 2 R 7 , SO,H, CONR 7 R 7 , CN, S0 2 C 1 NHS0 2 R 7 , PO(OR 7 ) 2 , NR 7 R , NR 7 COH, NR 7 COC 1 -Cgalkyl, NR 7 CON(R 7 ) 2 , NR 7 COW, W, S0 2 W; m is 0 or 2-4; 2 R is C 2 -C 10 alkyl, C 3 -C 1Q alkenyl, C 3 ~C, Q alkynyl, C 3 ~CgCycloalkyl, or (CH 2 ) 0 _gPhenyl unsubstituted or substituted by one to three substituents selected from C 1 ~C g alkyl, nitro, Cl, Br, F, I, hydroxy, C,-C 8 alkoxy, NR 7 R , C0 2 R 7 , CN, CONR 7 R 7 , W, tetrazol-5-yl, NR 7 COC 1 -C 8 alkyl, NR 7 COW, SC 1 -C 8 alkyl, S0 2 , or S0 2 C 1 -C 8 alkyl; X is a single bond, S, NR , or 0; 3 R is hydrogen, Cl, Br, F, I, CHO, hydroxymethyl, COOR 7 , CONR 7 R 7 , N0 2 , W, CN, NR 7 R 7 , or phenyl; R 4 and R5 are i.ndependently hydrogen, C^-C 8 alkyl, or phenyl-Y-, naphthyl-Y-, or biphenyl-Y-, wherein the aryl groups are unsubstituted or substituted by one to three substituents selected from Cl, Br, F, I, Cι~Cgalkoxy, hydroxy, CO2R 7 , CN, NO2, tetrazol-5-yl, SO3H, CF3, C0NR 7 R 7 , SO2NHR 7 , Ci-Cgalkyl, or NR 7 , R 7 ' ' or by methylenedioxy, phenoxy or phenyl, thienyl-Y-, furyl-Y-, pyrazolyl-Y-, imidazolyl-Y-, pyrrolyl-Y-, triazolyl-Y-, oxazolyl-Y-, isoxazolyl-Y-, thiazolyl-Y-, pyridyl-Y-, or the proviso that R 4 and R * * ** are not both selected from hydrogen; Y is .a single bond, 0, S, or C.,-C 8 alkyl which is straight or branched or optionally substituted by phenyl or benzyl, wherein each of the aryl groups is unsubstituted or substituted by halo, N0 2 , CF 3 , C,- Z is a single bond, vinyl, -CH p -O-CH--, methylene optionally substituted by C 1 ~C g alkyl, one or two benzyl groups, thienylmethyl, or furylmethyl, or -C(0)NHCHR -, 9 wherein R is H, C,-C 8 alkyl, phenyl, benzyl, thienylmethyl, or fur lmethyl; W is CnF2-n+..l, , CnF2_n+.-l, wherein n is 1-3; A is -(CH 2 ) m -, -CF=CH-, -0(CH 2 ) n -, or -S(CH 2 ) n ~; each R independeπ.-ly is hydrogen, C.-C 8 alkyl, or (CH 2 )m.p Q henyl, wherein m is 0-4; and R is hydrogen, C.-Cgalkyl, or 2-di amino-2-oxoethyl; or a pharmaceutically acceptable salt thereof. 2. A compound of claim 1 in which one of R 4 and R5 is hydrogen or C,-C.alkyl. 3. A compound of claim 2 in which R is phenyl unsubstituted or substituted by one to three substituents selected from chloro, fluoro, trifluoromethyl, nitro, methyl, methoxy, hydroxy, sulfamyl, cyano, carboxy, carboC.-C 8 alkoxy, carbamoyl, or tetrazol-5-yl, and m is 0 or 2 . 4. A compound of claim 3 in which X is a single 2 bond or S and R is C 2 -C 8 alkyl. 5. A compound of claim 4 in which R 3 is hydrogen, chloro, fluoro, or trifluoromethyl and R is hydrogen or C.-C.alkyl. g 6. A compound of claim 5 in which R is COOH, C00C 1 _ 2 alkyl or CONH 2 . 7. A compound of claim 6 in which R is thienylmethyl, furylmethyl, imidazolylmethyl, or pyridylmethyl, each of which is optionally substituted by methyl or methoxy C3-Cgalkyl; or benzyl unsubstituted or substituted by one to three substituents selected from Cl, Br, F, N0 2 , 0CH 3 , OH, CF3, NR 7 R 7 , CH3, or C0 2 R 7 , or methylenedioxy. 8. A compound of claim 7 which is the E isomer, wherein the R^ group and the imidazole are trans to each other. 9. The compound of claim 8 which is (E)-3-[2-n- butyl-l-{4-carboxyphenyl}-lH-imidazo-5-yl]-2-(2- thienyl)methyl-2-propenoic acid or a pharmaceutically acceptable salt thereof. 10. The compound of claim 8 which is (E)-3-[2-n- butyl-l-{ (4-carboxyphenyl)ethyl}-lH-imidazo-5-yl]-2-(2- thienyl)methyl-2-propenoic acid, or a pharmaceutically acceptable salt thereof. 11. A pharmaceutical composition comprising a pharmaceutical carrier and a compound of claim 1. 12. A pharmaceutical composition of claim 11 in which the compound is (E)-3-[2-n-butyl-l-{4- carboxyphen 1}-lH-imidazo-5-yl]-2-(2-thienyl)methy1-2- propenoic acid. 13. A pharmaceutical composition of claim 11 in which the compound is (E)-3-[2-n-butyl-l-{ (4-carboxy¬ phenyl)ethyl}-lH-imidazo-5-yl]-2-(2-thienyl)methyl-2- propenoic acid. 1 . A method of antagonizing angiotensin II which comprises administering to a subject in need thereof an effective amount of a compound of claim 1. 15. A method of treating hypertension which comprises administering to a subject in need thereof an effective amount of a compound of claim 1. 16. A method of treating congestive heart failure which comprises administering to a subject in need thereof an effective amount of a compound of claim 1. 17. A method of treating renal failure which comprises administering to a subject in need thereof an effective amount of a compound of claim 1. 18. A method of treating glaucoma which comprises administering to a subject in need thereof an effective amount of a compound of claim 1.

Inventors: FINKELSTEIN JOSEPH ALAN, KEENAN RICHARD MCCULLOCH, WEINSTOCK JOSEPH, FINKELSTEIN, JOSEPH, ALAN, KEENAN, RICHARD, MCCULLOCH, WEINSTOCK, JOSEPH
Applicants: SMITHKLINE BEECHAM CORP, SMITHKLINE BEECHAM CORPORATION
Application year: 1991
Main classification: A61K31
IPC codes: A61K31, A61P9, A61P13, A61P15, A61P27, A61P43, C07D233, C07D401, C07D403, C07D405, C07D409, C07D413, C07D417, C07F9