VACCINE COMPOSITIONS

Abstract: Improved vaccine compositions comprising a long chain alkyl compound as an immunoadjuvant in combination with a homogeneous immunogenic polypeptide. The compositions of the invention are useful in activating the immune system to confer immunity to a host against the immunogen in a prophylactic manner.

Description: VACCINE COMPOSITIONS The present invention relates to vaccine compositions having improved immunogenicity. More particularly, the compositions of the invention comprise a polypeptide immunogen in combination with a long chain alkyl compound which functions as an immunoadjuvant. BACKGROUND OF THE INVENTION It is well known that vaccines are important in the prophylaxis of disease. Vaccines operate by exposing a host animal to foreign material designed to activate the immune system to confer upon the host immunity against the material without exposing the host to the risk of disease. At the present time, about 20 vaccines have been developed for commercial use. Most of those vaccines are made by detoxification of the disease-causing organism, or a portion of that organism, by one of several methods such as, for example, isolation of a specific non-toxic portion of the organism or by treatment of a toxic portion of the organism with chemicals, heat or genetic attenuation. The detoxification procedure often produces heterogenous products contaminated with toxic by-products of uncertain composition. As a result, the effectiveness of the impure detoxified vaccines as immunogenic materials may be impaired. In addition, unpleasant and sometimes permanently damaging side effects can result. A well known example is the use of intact B. pertussis bacteria as the basis for a vaccine for childhood whooping SUBSTITUTE SHEET cough. The vaccine currently used in the United States is essentially the same version introduced in the 1940' s. Side effects vary from minor reactions to claimed instances of paralysis, permanent brain damage and even death. The advent of recombinant and other sophisticated chemical technologies has facilitated the development of purer and hence safer vaccines. However, because of this improved purity, which frequently means that natural immunostimulants have been removed, these new vaccines are often weak, or poorly immunogenic, especially when compared to their predecessors. In order to offset that effect, immunoadjuvants are employed in association with the vaccines to elicit enhanced antibody formation. Presently, only aluminum and calcium salts are employed as adjuvants for commercial vaccines. However, aluminum and calcium salts are not potent immunoad uvants. Calcium salts have found only limited use and aluminum salts may provoke transient or chronic local granulomas at the site of injection. L.H. Collier, in Lancet at pages 1364-1367 (1970), states that the incidence and severity of reactions to tetanus toxoid vaccine depends upon the purity of the antigen as well as t.he presence of aluminum adjuvant. The preparation of aluminum adjuvants is not always reproducible. Moreover, aluminum can alone stimulate the production of IgE antibodies which are responsible for mediating immediate hypersenεtive reactions. This has been described by T. Matuhasi, et al, J. Infectious Disease, 146, 290 (1982). Attention has focused in recent years on the use of organic compounds as adjuvants. Only a few organic compounds function in a manner similar to commercially acceptable inorganic salts, i.e. as a slow release vehicle or antigen (vaccine) depot whereby antigen is released over a relatively long period of time at the site of injection. Examples of such organic compounds are organic surfactants and emulsifiers, such as Pluronics and Tetronics which are non-ionic block copolymers of polyoxyethylene and polyoxypropylene produced by BASF Corporation. Such a slow-release mechanism of adjuvanticity has long been accepted for human use since it reduces the possibility of overstimulating the immune system. Overstimulation of the immune system can lead to an autoimmune response such as would occur with the use of a potent immunostimulant, for example Freund' s adjuvant. Thus, the slow-release mechanism is the preferred mechanism. While the majority of organic adjuvants have been shown to be potent immunostimulants, such highly active adjuvants tend to be toxic and, therefore, unacceptable for human use. Examples of known organic adjuvants are Freund' s complete adjuvant and muramyl dipeptide which are potent immunostimulants, but both of those compounds are restricted to use in animal research because of toxicity considerations. Many of the organic adjuvants which mimic aluminum salts are more toxic than aluminum salts. For example, long chain alkyl amines described by D. Gall in Immunology, 11, 369-386, 1966 are reported to be toxic compounds which are generally disruptive to cell membrane structure. It is known that large high molecular weight heterogenous antigens of diverse composition will complex with insoluble organic molecules the latter of which function as adjuvants. This is a non-specific binding phenomenon in that, if the antigenic material is large and diverse enough, it will bind to any insoluble organic molecule, regardless of charge and polarity. The complexation between the organic adjuvant and the biomolecule occurs through a variety of weak, non-covalent forces, such as hydrophobic interactions and hydrogen bonding. This phenomenon is seen in U.S. patent 4,428,932 to Overell and U.S. patent 4,258,0294 to Moloney et al. Overell discloses that alkyl tyrosine functions as an adjuvant for allergy desensitization therapy when complexed with heterogenous multi-component high molecular weight allergens, such as rye, grass and pollen extract. Moloney et al teach the use of long chain alcohol esters of amino acids as adjuvants when complexed with heterogeneous multi-component high molecular weight tetanus toxoid and type I, II and III poliomyelitis virus. A need exists therefore to develop non-toxic vaccine-adjuvant compositions having improved immunogencity and in which both the immunogen and the adjuvant are non-toxic. SUMMARY OF THE INVENTION The present invention centers on improved vaccine compositions comprising a homogeneous immunogenic polypeptide and a non-toxic long chain alkyl immunoad uvant. Thus, according to one aspect of the present invention, there is provided a vaccine composition comprising a homogeneous immunogenic polypeptide and an adjuvant which is a non-toxic long chain alkyl compound present in an amount effective to amplify the immunogenicity of the polypeptide. According to another aspect of the invention, there is provided a method of eliciting an immune response comprising administering to a warm blooded animal, including humans, an effective amount of a vaccine composition of the invention. It has surprisingly been found, according to the present invention, that non-toxic positively charged long chain alkyl compounds, in particular esters of amino acids or peptides, can bind homogeneous polypeptides in a selective manner. This is a specific phenomenon that depends upon the type of amino acid present in the insoluble ester as well as the type of homogeneous polypeptide. This unexpected specificity is characteristic of the homogeneous polypeptides employed according to the invention, and is not exhibited by polysaccharides of similar molecular weight. It has also been found according to the present invention that when the standard aluminum-containing adjuvant is replaced by a non-toxic long chain alkyl compound as adjuvant, typically a long chain alkyl amino acid ester, one or more effects regarding the isotype of antibody produced may possibly occur. One effect concerns IgE antibody production. Specifically, when the long chain alkyl adjuvanted compositions of the invention are used, levels of IgE antibody are not substantially raised and remain essentially at levels observed when no adjuvant is used, as compared to the elevated IgE level observed when aluminum-containing adjuvants are used. IgE antibodies are responsible for mediating immediate hypersensitivity reactions. There have been reports of anaphylatic reactions and even death as a result of IgE production in response to immunization with aluminum adjuvanted vaccines. See Matuhasi, et al, discussed above. Therefore, the reduced levels of IgE formation observed with the long chain alkyl adjuvanted compositions of the invention is a beneficial therapeutic effect. Another effect is that the ratio of IgG2a antibody to IgGl is higher in that both antibody isotype levels increased when the long chain alkyl adjuvants are used in the compositions of the invention as opposed to the ratio observed when aluminum adjuvants are used. This ratio increase is also a beneficial effect since IgG2a antibody is important as being the most effective murine antibody in regard to activation of complement and antibody-dependent cellular cytotoxicity mechanisms and protection against tumors and parasites. The term "homogeneous" as used herein to define polypeptides employed in the vaccine compositions of the invention means an immunogenic polypeptide species consisting essentially of one or more epitopes of a pathogenic agent prepared by chemical synthesis or biological synthesis. In the event that more than one epitope is present, then the homogeneous immunogenic polypeptide would comprise the immunodominant epitope or epitopes. Chemical synthesis is generally employed when one epitope is present, whereas either chemical or biological synthesis may be used in the situation where more than one epitope is present. The homogeneous polypeptide may comprise other portions of the pathogenic agent which are non-toxic and do not adversely affect the immunogenicity of the homogeneous polypeptide. The non-toxic adjuvant is preferably a positively charged ester of an amino acid or peptide, in particular an ester of an alkyl alcohol containing 14 to 20 carbon atoms and an amino acid, dipeptide, or tripeptide. DETAILED DESCRIPTION OF THE INVENTION The homogeneous immunogenic polypeptide employed in the compositions of the invention is non-toxic and is capable of eliciting an immune response in the host. The immunogenic polypeptide is comprised substantially of one or more epitopes derived from pathogenic agents responsible for AIDS, hepatitis A, hepatitis B, hepatitis C, tetanus, polio, pertussis (Lymphocytosis Promoting Factor (LPF) toxin), herpes simplex, respiratory syncytial virus, measles, influenza virus, rabies, lassa fever, rotavirus, adenovirus, rhinovirus, foot and mouth disease, bovine and feline leukemia virus, rinderpest virus, dengue fever virus, tick-borne encephalitis, malaria and parainfluenza virus. In many instances, it is desirable to modify the naturally occurring amino acid sequence of the polypeptide iirammogen in order to make the homogeneous polypeptide more useful in vaccine compositions, while not changing its immunogenic properties. Examples of such changes include modifications of the amino acid side chains in order to mimic post-translational modifications or to alter the chemical properties of the immunogen. Modifications of the polypeptide in order to improve the lifetime of the immunogen in vivo are also possible. Moreover, addition of a cysteine residue at the amino or carboxyl terminus of the polypeptide in order to facilitate coupling of the polypeptide to a carrier protein is possible. These changes or modifications may be insertions, deletions and substitutions, either conservative or non-conservative. Such changes include combinations of the naturally occurring amino acids used for protein synthesis, such as gly, ala; val, ile, leu; asp, glu; asn, gin; ser, thr; lys, arg; phe, tyr; ala, ser; ala thr; ala, val; ala, pro; ala, glu; leu, gin; gly, phe; ile, ser; and ile, met. The immunogenic homogeneous polypeptide may be produced by any convenient method. Thus, the polypeptide may be produced by recombinant or synthetic techniques, or combinations thereof. Known recombinant techniques are described in Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1982), T. Maniatis et al. For example, a cell may be designed to overexpress a desired polypeptide through transfection with a gene which codes for that polypeptide. Suitable chemical synthesis methods include solid- or solution-phase peptide synthesis or enzymatic synthesis. Suitable procedures are described in Principles of Peptide Synthesis, Springer Verlag, 1984, M. Bodanszky; Solid Phase Peptide Synthesis, second edition, 1984, Pierce Chemical Company, John Morrow Stuart and Janis Dillaha Young. The polypeptide may be either truncated, elongated or otherwise modified by known techniques. Moreover, the polypeptide may be either linear or cyclic. Purification of the immunogenic polypeptide may be carried out using any commonly employed technique. Any chemical or biosynthetic method which destroys or removes other biomolecules and unwanted material, and leaves the desired immunogenic polypeptide unaffected may be used. Preferably, the immunogenic polypeptide is purified by chromatographic techniques. In particular, chromatographic techniques such as size exclusion, ion exchange, affinity or, most preferably, high performance liquid chromatography, or mixtures thereof, may be employed. The purity of the immunogenic polypeptide may be verified by gel electrophoresis. The immunogenic polypetides should be of sufficient size to be recognized by the host and identified as foreign. An immunogen usually has a molecular weight of at least 10,000 daltons. If the polypeptide is below the critical size the particular host will recognize, the polypeptide must be coupled to a carrier that will render the polypeptide-carrier conjugate immunogenic. Preferable carriers are proteins. Preferred carriers for animal use are bovine serum albumin and Keyhole Limpet Hemocyanin (KLH) . Carriers suitable for human use include tetanus toxoid, diphtheria toxoid, acellular •pertussis vaccine (LPF), cross-reacting materials (CRN's) which are antigenically similar to bacterial toxins but are non-toxic by means of mutation, preferably CRM, Q7 , obtained according to Pappenheimer, et al, An Immunological Study of the Diphtheria Toxin Molecule, Immunochemistry 9, 891-906 (1972), and other bacterial protein carriers, for example meningococcal outer membrane protein. Preferably, the carrier protein itself is an immunogen. The polypeptide may be coupled to the carrier by any convenient method known to the art. While use of a symmetric linker such as an alkyl dialdehyde (e.g. glutaraldehyde) or an alkyl diimidate (e.g. dimethyl suberimidate) is within the scope of the invention, it is preferred to use a heterobifunctional linker. The latter insures that linkage of the polypeptide to the carrier occurs in a defined and reproducible manner, by avoiding side-reactions such as cross-linkage of carrier molecules which would lead to impure heterogeneous preparations. Examples of preferred heterobifunctional linkers include N-succinimidyl 3-(2-pyridyldithio) propionate as described by J. Carlsson et al; BioChem, J. 173, 723-737 (1978), and sulfosuccinimidyl 4-(p-maleimidophenyl)butyrate, as described by J.D. Bangs, et al; J. Cell Biology 103, 255-263 (1986). Most preferred is sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-l-carboxylate, as described by S. Hashida, et al; J. Applied Biochem 6, 56-63 (1984). Examples of suitable homogeneous polypeptides are those consisting essentially of substantially the same amino acid sequence as a surface antigen of a hepatitis virus, such as the homogeneous polypeptide consisting essentially of substantially the same amino acid sequence as hepatitis B surface antigen. Another example is a homogeneous polypeptide consisting of one or more epitopes of a surface antigen of hepatitis. Further examples are the homogeneous polypeptide consisting essentially of substantially the same amino acid sequence as a surface antigen of the gD subunit of a herpes simplex virus, the homogeneous polypeptide consisting essentially of one or more epitopes of gpl20 of a human immunodeficiency virus, the homogeneous polypeptide consisting essentially of one or more epitopes of gp41 of a human immunodeficiency virus, or the homogeneous polypeptide consisting essentially of substantially the same amino acid sequence as Lymphocytosis Promoting Factor (LPF). The long chain alkyl adjuvant, as well as any compounds which arise from its metabolism in the host, should be non-toxic. It is well known that long chain fatty alcohols are naturally occurring non-toxic substances. As an example, octadecanol is found to be totally non-toxic in humans, as is indicated by an oral LD,_ n which is greater than 15 g/kg, as found in Gosselin's "Clinical Toxicology of Commercial Products", Fourth Edition, 1976. Octadecyl tyrosine has been found to be non-toxic in animals and the majority of naturally occurring amino acids are non-toxic. C.L. Penney, et al, Vaccine, 4, 99-10, 1986. It would therefore be expected that octadecyl tyrosine and esters of other alcohols and amino acids would not exhibit any toxicity in humans. The adjuvant should be capable of forming microparticles having a size of between about 150 μ m-lmM (mesh 18 - mesh 100, preferably about 250 μm (mesh 60) in an aqueous medium, thereby giving rise to a suspension of uniform consistency. Moreover, the adjuvant microparticles should permit adsorption of the immunogen, thereby allowing the slow release of immunogen into the host. In a preferred embodiment of the present invention, the adjuvant is a compound of formula: I D where C is a hydrogen atom, an amino acid residue, or peptide residue including up to ten amino acid residues (i.e. up to a decapeptide) ; D is a hydrogen atom, or a pharmaceutically acceptable acid such as hydrochloric, hydrobromic, phosphoric, sulfuric, tartaric, lactic or acetic acid; E is 4-hydroxybenzyl, benzyl, 4-hydroxyphenyl, phenyl, 4-aminobutyl, isopropyl, methyl, hydrogen, or other residue of a naturally occurring amino acid; A is (CH-) , oxygen or CH 2 ° and B is ^ CH 2^ or oxygen, where n is 0 to 4, but A ≠ B for (CH_) or oxygen; and R is an alkyl group containing 12 to 20 carbon atoms. Preferably, C may be either hydrogen, an amino acid, a dipeptide or a tripeptide. If C is an amino acid, the amino acid sequence of the adjuvant may be selected from for example tyrosyl glycine, glycyl glycine, glycyl tyrosine, or phenylalanyl glycine. If C is a dipeptide, the amino acid sequence of the adjuvant may be selected from for example tyrosyl glycyl glycine or tyrosyl alanyl glycine. If an amino acid residue is chiral, the D-enantiomer, the L-enantiomer, or mixtures thereof may be employed. It is particularly preferred for the adjuvant to comprise an alpha amino acid. It is particularly preferred for E to be selected from 4-hydroxybenzyl, benzyl, 4-hydroxyphenyl, phenyl and hydrogen. E is most preferably 4-hydroxybenzyl . When A is CH 2 ° and B is ^ CH 2^n' the compounds are N-aminoacylethanolamine-O-stearates. When A is CH ? 0 and B is oxygen, the compounds are carbonates. More preferably, the adjuvant is an amino acid ester hydrochloride where C is hydrogen, D is hydrochloric acid, A is (CH 9 ) , where n is 0-4, and B is oxygen. Most preferably, the adjuvant is octadecyl tyrosine hydrochloride where C is hydrogen, D is hydrochloric acid, E is 4-hydroxybenzyl, and R is octadecyl, A is (CH_) where n is zero and B is • * v 2'n oxygen. Generally, when C is not hydrogen, the backbone of the adjuvant comprises substantially peptide ■bonds, i.e., the carboxylate of one ' amino acid residue is linked directly to the amino of the adjacent residue, in a head-to-tail manner. Alternatively, the peptide bond may be a thioa ide. The adjuvant may be prepared by any convenient method. For example, the amino ester portion of the adjuvant may be synthesized by any one of a number of established methods, as has been described by M. Bodanszky et al; "Peptide Synthesis" Second Edition, Wiley, New York, 1976, and R.W. Roeske, Peptides (N.Y.) 3, 102 (1981). A particularly preferred method is the methanesulfonic acid catalyzed esterification procedure described by C. Penney et al, J. Organic Chemistry 50, 1457-1459 (1985). When the adjuvant is a di- or tripeptide, the peptide bonds may be formed by any of the procedures described in "Peptides Synthesis", mentioned above. Additionally, the peptide bonds may be formed following either solid or solution phase protocols. Many protocols and reagents exist which are useful in forming amide, thioamide, or thioester bonds. During the preparation of the adjuvant, it may be desirable to temporarily protect reactive functional groups. For example, amines may be protected by urethane-type groups, alcohols by t-butyl or benzyl groups, and acids by ester groups. Suitable protection/deprotection conditions and protocols are described in "Peptide Synthesis" mentioned above. The adjuvant may be purified by any of the techniques described previously. The preferred purification technique is silica gel chromatography, in particular the "flash" (rapid) chromatographic technique, as described by W. Clark Still et al, J. Organic Chemistry, 43, 2923-2925 (1978). However, other chromatographic methods, including HPLC, may be used for purification of the adjuvant. Crystallization may also be used to purify the adjuvant. In some cases, no purification is required as a product of analytical purity is obtained directly from the synthesis. The vaccine compositions of the invention are prepared by physically mixing the adjuvant with the homogeneous immunogenic polypeptide under appropriate sterile conditions in accordance with known techniques to produce the adjuvanted composition. As will be clear from the above discussion, the immunogenic polypeptide will be mixed with the adjuvant either on its own or coupled with a suitable carrier. The amounts of the adjuvant and homogeneous polypeptide immunogen, either alone or coupled to a carrier, needed to elicit an immune response in humans are interrelated, but are within the ranges generally employed in conventional vaccines. For example, the use of increasing amounts of adjuvant may suggest that decreasing amounts of immunogen can be used, and vice versa. The preferred amount of adjuvant is 0.01 to 5 mg/ml of the composition, for example 0.05 mg/ml to 3 mg/ml, preferably 0.5 to 1.0 mg/ml. The preferred amount of immunogen is between about 1 to 100 micrograms/ml, preferably about 5 to 40 micrograms/ml. The dosage will depend upon the host receiving the vaccine as well as factors such as the size, weight, and age of the host. The vaccine compositions of this invention may be formulated using techniques similar to those used for other pharmaceutical polypeptide compositions. Thus, the adjuvant and immunogen may be stored in lyophilized form and reconstituted in a physiologically acceptable vehicle to form a suspension prior to administration. Alternatively, the adjuvant and immunogen may be stored in the vehicle. Preferred vehicles are sterile solutions, in particular, sterile buffer solutions, such as phosphate buffered saline. Any method of combining the adjuvant and the immunogen in the vehicle that retains the immunoreactivity of the mixture is appropriate. The vehicle may contain preservatives or other known additives which are used to improve the shelf stability or the efficacy of the mixture. Suitable preservatives include, for example, thimerosal. The volume of a single dose of the vaccine of this invention may vary but will be generally within the ranges commonly employed in conventional vaccines. The volume of a single dose is preferably between about 0.1 ml and about 1.5 ml, more preferably between about 0.2 ml and about 0.5 ml at the concentrations of immunogen and adjuvant noted above. The vaccine compositions of the invention may be administered by any convenient means. Preferred methods of administration include subcutaneous, intramuscular, intradermal, or by way of nasal delivery. Alternatively, the mixture may be released from a biodiffusible implant. A single administration may be used. Alternatively, a series of administrations may be made over the course of • several days or weeks. EXAMPLES The following non-limiting examples illustrate the invention. Example 1 This example describes the preparation of homogeneous Hepatitis B surface antigen (HBsAg) . Recombinant HBsAg was isolated from human 3T3 cells which were transfected with the surface antigen of the Hepatitis B virus provided by the Mount Sinai School of Medicine (Sells, et al, Production of Hepatitis B virus particles in HepG2 cells transfected with cloned Hepatitis B virus DNA. Proc. Natl. Acad. Sci. USA, 84,1005 (1987)). Cells were grown in RPMI media (Gibco) supplemented with 10% FCS (Gibco), ImM sodium pyruvate and 0.5% genta ycin. At this point, two purification procedures may be employed, namely density gradient centrifugation or immunoaffinity chromatography. In density gradient centrifugation, the cell supernatant is spun at 2000 rpm for 10 minutes to remove any 3T3 whole cells, and then the antigen is purified by precipitation using using polyethylene glycol (MW 8000) at a concentration of 10% (w/v) . The resulting pellet is resuspended in TNE buffer (10 mM Tris. HC1 (pH 8.0), 100 mM NaCl 1 mM EDTA (pH 8.0)) and centrifugation is performed in a gradient of 20% to 50% (wt/vol) cesium chloride in TNE buffer in SW41 polyallo er tubes. The gradients are centrifuged at 35,000 rpm for 60 hours at 4°C. Fractions are collected from the bottom of the tube and the density is determined using a refractometer. The fractions of a density (1.351 - 1.358) are pooled and dialysed against PBS, concentrated and analyzed for homogeneous HBsAg concentration using an Abbot RIA kit using the positive control as equivalent to 20 ng/ml of antigen. The immunoaffinity chromatography method is generally carried out by spinning the cell supernatant at 2,000 rpm for 10 mins to remove any 3T3 whole cells. An anti-HBsAg affinity column, prepared by covalently linking anti-HBsAg monoclonal antibody to cyanogen bromide activated Sepharose CL4B, is washed with PBS. The 0D or . of eluant is checked, and should be < 0.010. ( v 0D28 o 0» nm = Optical Density (Absorbence) at 280 nm) . The supernatant is run through the column twice to ensure that the antigen is absorbed onto the column. The column is then washed 3 times to remove any non-specific binding of proteins to the column (check O.D.2,,8_.,0- nm < 0.01) ', followed by J elution with glycine/HCl (pH 2.8). Ten tubes are prepared with sufficient tris buffer (pH 11) in each tube to neutralize the 2ml fraction of glycine/HCl. The ten tubes are analysed by O.D- Q .. nm or by measuring HBsAg by RIA. Fractions containing the homogeneous polypeptide are combined and dialyzed against PBS. The column is washed 3 times with PBS. The column is stored in PBS/Na azide. Example 2 The following describes the general method for the complexation of a homogeneous polypeptide immunogen with a long chain alkyl amino acid ester immunoadj vant. The long chain alkyl amino acid ester immunoadjuvant (50mg) was placed in a 25 ml volumetric or small erlenmeyer flask with spin-bar, and stoppered with a cotton plug, or other loose fitting material. The salt form of the immunoadjuvant was sterilized by heating in an oven at an appropriate temperature, as dictated by the melting point, for 20 hours. To the cooled flask was added phosphate buffered saline (25ml), sterilized by filtration, >50 mM, pH = 6-7. The resulting suspension was gently stirred for 3-20 hours, the plug replaced with a glass stopper, and the whole stored at 4°C until required for complexation. In order to complex the immunoadjuvant with the homogeneous polypeptide, the suspension was brought to room temperature, and thorougly agitated. The required volume of suspension was transferred with a wide-bore pipet to an equal volume of polypeptide solution, so that the final concentration of immunoadjuvant was 1 mg/ml. However, volume adjustments could be made as required to give any other desired concentration of immunoadjuvant. The immunoadjuvant-polypeptide suspension was gently mixed overnight at 4°C. At the end of the complexation reaction, the suspension was allowed to settle, centrifuged, if necessary, and the absorbance at 280 nm of the supernatent measured to determine the amount of unbound polypeptide. Generally, 30%-90% bound polypeptide represents a good immunoadjuvant-polypeptide immunogen complex. Example 3 This example demonstrates the adjuvanticity of several octadecyl amino acid esters with a homogeneous polypeptide which is a candidate vaccine for hepatitis. BALB/c mice were immunized with 50 ng of hepatitis B surface antigen in the presence of adjuvant for the non-control mice and in the absence of adjuvant for the control mice. The hepatitis B surface antigen was obtained from 3T3 cells transfected with the gene for the surface antigen. 100 μg of octadecyl amino acid was complexed with the polypeptide as described in Example 2. This mixture, in a total volume of 0.1 ml, was injected on day 1 using an intramuscular route, and the mice were bled on days 7, 14, 21, 28, 35, 42 and 61. The mice were boosted with 35 ng of the polypeptide on day 21. The antibody concentration in the sera was determined by radioimmunoassay. The antibody concentration was estimated by comparison with a standard curve obtained using a monoclonal antibody. The results are presented in Table 1. TABLE 1 Antibody response to hepatitis B surface antigen in the presence of octadecyl amino acid immunoadjuvant DAY* Adjuvant 1 14 21 28 35 61 Saline 43 36 32 61 59 58 Alum 44 53 46 163 320 516 Octadecyl Tyrosine 68 91 58 355 833 752 Octadecyl /3-Alanine 31 45 44 120 337 373 Octadecyl Glycine 47 38 51 147 748 301 Octadecyl Proline 49 35 41 72 409 168 Octadecyl Leucine 48 42 37 160 590 496 Octadecyl Lysine 33 55 39 51 67 53 Octadecyl Forphenicinol 31 40 26 65 306 98 Octadecyl Forphenicine 100 112 116 908 592 355 *Anti-HBsAg antibody concentration, ng/ l The hepatitis B surface antigen amino acid sequence for amino acids 1 through 226 (HBsAg - adw (S gene product) is described in Bhatnagar P.K., et al, Proc. Natl. Aca. Science, USA vol. 79, 4400-4404 (1982), specifically incorporated by reference. The results in Table 1 show that the adjuvant effect observed between long chain alkyl amino acid esters and an immunogenic polypeptide, hepatitis B surface antigen, is a specific phenomenon. This can be seen by comparison of octadecyl tyrosine and SUBSTITUTE SHEET octadecyl lysine, and by comparison of octadecyl forphenicinol and octadecyl forphenicine. Example 4 This example demonstrates the adjuvanticity of octadecyl tyrosine as a function of dose with a polypeptide which is a candidate vaccine for hepatitis. The protocol was as described above, except that mice were immunized with 80 ng of hepatitis B surface antigen, boosted with 50 ng, and bled on days 7, 14, 28 and 35. The immunizations were carried out in the presence of adjuvant for the non-control mice and in the absence of adjuvant for the control mice. The results are presented in Table 2. TABLE 2 Antibody response to hepatitis B surface antigen in the presence of octadecyl tyrosine *CPM, pre-bleed value was 11.5 The results in Table 2 show that the adjuvant effect is dependent upon the concentration of long chain alkyl ester, octadecyl tyrosine, present in the composition. As the amount of adjuvant administered increases, a proportional increase in the antibody titer occurs until an optimum titer value is reached, beyond which the antibody titer does not increase. Example 5 This example demonstrates the ad uvanticity of octadecyl tyrosine as a function of dose, and in different animals, with a polypeptide which corresponds to a fusiogenic sequence from the envelope protein of the human immunodeficiency virus, HIV-1. 1) HIV Peptide Sequence The peptide sequence of the HIV was as follows. Product: BC-101 303- H-Val-Ala-Pro-Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg- Glu-Lys-Arg-Ala-Val-Gly-Ile-Gly-Ala-Leu-Phe-Leu-Gly-Phe- Leu-Gly-Ala-Ala-Gly-Ser- 535 M. . 3585.14 (Back-bone) 2) Experiments in mice BALB/c mice were immunized with 100 μg of chemically synthesized polypeptide, corresponding to the amino acid sequence of residues 503-535 of HIV-1, coupled to Keyhole Limpet Hemocyanin (KLH). Octadecyl tyrosine hydro-chloride, 10 μg-100 μg, was complexed with the polypeptide as described in Example 2. This mixture, in a total volume of 0.1 ml, was injected on day 1, and again on day 21. The mice were bled on days 7, 14, 21, 28 and 35. SUBSTITUTE SHEET The antibody concentration in the sera was determined by radioimmunoassay. The antibody concentration was estimated by comparison with a standard curve obtained using a polyclonal antibody. The results are presented in Table 3. TABLE 3 Antibody response to HIV-1 polypeptide, residues 503-535, in the presence of octadecyl tyrosine *Anti-peptide antibody concentration, μg/ml The results in Table 3 show that a long chain alkyl ester, octadecyl tyrosine, exhibits a concentration dependent adjuvant effect with another immunogenic polypeptide, namely a polypeptide derived from amino acids 503 to 535 of the HIV protein gp41. Again, an optimum antibody titer is reached, beyond which the titer does not increase. 3) Experiments in baboons Two baboons were immunized with 300 μg of chemically synthesized polypeptide, corresponding to the amino acid sequence of residues 503-535 of HIV-1, coupled to Keyhole Limpet Hemocyanin. 500 μg of octadecyl SUBSTITUTE SHEET tyrosine hydrochloride was complexed with the polypeptide, as described in Example 2. This mixture in a total volume of 1.0 ml was injected on day 1 and again on day 35. The animals were bled 3 days before the first immunization, and on days 14, 32 and 49. The same experiment was undertaken with another two baboons, but with polypeptide corresponding to the amino acid sequence 526-535 of HIV-1. The antibody concentration in the sera was indirectly measured by radioimmunoassay. Tiltration was undertaken by incubation of samples on plates coated with peptide-albumin conjugate, and reaction with iodine-125 labelled rabbit anti-monkey immunoglobulin. The results are presented in Table 4. TABLE 4 Antibody response to HIV-1 polypeptide in baboons in the presence of 500 μg octadecyl tyrosine hydrochloride ANIMAL 1 ANIMAL 2 DAY* DAY* Polypeptide __^ ±4. H ϋ 2 : _32 4_9 503-535 1020 2004 2185 5948 1530 2160 2121 4470 526-535 300 686 1685 3121 220 500 965 4373 *CPM, after subtraction of background on bovine serum albumin coated plates The results in Table 4 show that the adjuvant effect between a long chain alkyl ester, octadecyl tyrosine, and an immunogenic HIV polypeptide is observed in higher animals, for example baboons. SUBSTITUTE SHEE Example 6 This example demonstrates the change in isotype which occurs in going from an aluminum adjuvanted to an octadecyl tyrosine adjuvanted candidate vaccine for hepatitis. The protocol was as described in Example 5, Section 2 (mice) except that 50 μg of octadecyl tyrosine was used for both the primary and secondary (day 21) immunizations. The isotype of the antibody response was determined by radioimmunoassay. Use was made of tritium labelled anti-mouse isotype antibody. Titration was undertaken by incubation of samples on plates coated with immunoaffinity purified hepatitis B surface antigen. The results reported as radioactivity (cpm) of bound isotype antibody, are presented in Table 5. SUBSTITUTE SHEET Table 5 Isotype variation of the anti-hepatitis B surface antigen antibody response Immunization day of bleed IgM IgG3 IgGl IgG2b IgG2a IgE on day 1 and 21 in -1 587+81* 201+36 205+41 324+42 471+80 30+11 Saline 14 475+102 216+38 208+11 185+29 286+66 19+33 35 568+50 409+85 2541+127 1453+99 2856+448 59+33 42 437+84 154+20 1926+129 628+59 1486+173 34+8 Alum 14 230+8 123+10 417+107 280+34 333+41 29+1 35 693+48 1750+125 3342+352 3100+650 2808+73 706+106 42 272+149 138+24 2048+142 1782+166 2378+57 1825+154 OT 14 347+37 144+19 568+32 290+31 483+44 33+10 35 826+8 333+17 8030+155 4710+115 12331+336 55+33 42 545+87 165+24 6176+496 1109+77 2271+215 29+12 Isotype control* 11299+349 9799+52 3572+245 5951+281 3934+272 1565+221 27 / 1 The results shown in Table 5 show the change in isotype of the antibody response which occurs in going from aluminum to long chain alkyl ester, SUBSTITUTE SHEET 28 (octadecyl tyrosine - OT) adjuvanted immunogenic polypeptide, hepatitis B surface antigen. In particular, it is to be noted that the levels of IgE antibodies are elevated when aluminum is used, whereas there is no significant increase in IgE levels, as compared to that obtained with the control, when octadecyl tyrosine (OT) is used.

Claims: 29 CLAIMS 1. An vaccine composition comprising a homogeneous immunogenic polypeptide and an effective amount of at least one adjuvant of the formula: D wherein C is selected from the group consisting of hydrogen, an amino acid residue, and a peptide residue; D is selected from the group consisting of hydrogen and a pharmaceutically acceptable acid; E is selected from the group consisting of 4-hydroxybenzyl, benzyl, 4-hydroxyphenyl, phenyl, 4-aminobutyl, isopropy1, methyl, hydrogen and a residue of a naturally occurring amino acid; A is (CH„) , oxygen or CH«0 and B is (CH~) or oxygen, wherein n is 0 to 4, with the proviso that A and B are not the same for (CH 2)n and oxygen; and R is alkyl of 12 to 20 carbon atoms. 2. A composition according to claim 1, wherein said adjuvant comprises an amino acid having an L-configuration. 3. A composition according to claim 1, wherein ' said adjuvant comprises an amino acid having a D-configuration. 30 4. A composition according to claim 1, wherein said adjuvant comprises an amino acid mixture of D and L- configurations. 5. A composition according to claim 1, wherein E is selected from the group consisting of 4-hydroxybenzyl, benzyl, 4-hydroxyphenyl, phenyl, and hydrogen. 6. A composition according to claim 5, wherein E is 4-hydroxybenzyl. 7. A composition according to claim 1, wherein C is selected from the group consisting of hydrogen, an amino acid, and a peptide residue including up to ten amino acid residues. 8. A composition according to claim 7, wherein said peptide residue is selected from a dipeptide and a tripeptide. 9. A composition according to claim 7, wherein C is an amino acid and is selected from the group consisting of tyrosyl glycine, glycyl glycine, glycyl tyrosine, and phenylalanyl glycine. 10. A composition according to claim 8, wherein C is a dipeptide and is selected from the group consisting of tyrosyl glycyl glycine and tyrosyl alanyl glycine. 11. A composition according to claim 1, wherein the pharmaceutically acceptable acid is selected from the group consisting of hydrochloric, hydrobromic, phosphoric, sulfuric, tartaric, lactic, and acetic acid. 12. A composition according to claim 1, wherein said adjuvant comprises an α-amino acid. 13. A composition according to claim 1, wherein said homogeneous polypeptide is selected 31 from the group consisting of a linear and a cyclic polypeptide. 14. A composition according to claim 1, wherein said homogeneous polypeptide is coupled to a biological carrier. 15. A composition according to claim 14, wherein said carrier is selected from the group consisting of tetanus toxoid, diphtheria toxoid, 10 acellular pertussis vaccine (LPS), a cross-reacting material (CRM) and a bacterial protein carrier. 16. A composition according to claim 15, wherein said CRM is CRM.. q7 . 17. A composition according to claim 1, wherein -"- ■ said homogeneous polypeptide is comprised substantially of one or more epitopes derived from pathogenic agents responsible for a condition selected from AIDS, hepatitis A, hepatitis B, hepatitis C, tetanus, polio, pertussis on - u (Lymphocytosis Promoting Factor toxin), herpes simplex, respiratory syncytial virus, measles, influenza virus, rabies, lassa fever, rotavirus, adenόvirus, rhinovirus, foot and mouth disease, bovine and feline leukemia virus, rinderpest virus, t -" J dengue fever virus, tick-borne encephalitis, malaria and parainfluenza virus. 18. A composition according to claim 1, wherein said homogeneous polypeptide has substantially the same amino acid sequence as a surface antigen of a 30 hepatitis virus. 19. A composition according to claim 1, wherein said homogeneous polypeptide comprises one or more epitopes of a surface antigen of a hepatitis virus. 20. A composition according to claim 1, wherein 32 said homogeneous polypeptide comprises substantially the same amino acid sequence as hepatitis B surface antigen. 21. A composition according to claim 1, wherein said homogeneous polypeptide has substantially the same amino acid sequence as a surface antigen of the gD subunit of a herpes simplex virus. 22. A composition according to claim 1, wherein said homogeneous polypeptide comprises one or more epitopes of gpl20 of a human immunodeficiency virus. 23. A composition according to claim 1, wherein said homogeneous polypeptide comprises one or more epitopes of gp41 of a human immunodeficiency virus. 24. A composition according to claim 1, wherein said homogeneous polypeptide has substantially the same amino acid sequence as Lymphocytosis Promoting Factor. 25. A composition according to claim 1, wherein said adjuvant is an ester of an alkyl alcohol containing 14 to 20 carbon atoms and an amino acid, dipeptide or tripeptide. 26. A composition according to claim 25, wherein said adjuvant is octadecyl tyrosine. 27. A method of eliciting an immune response in a patient, said method comprising the step of administering to said patient a therapeutically effective amount of a vaccine composition of claim 1. 28. A method according to claim 27, wherein said composition is administered intramuscularly, intradermally, subcutaneously, or by way of nasal ' delivery. 29. A method according to claim 27, wherein 33 administration of said vaccine composition does not substantially raise IgE antibody levels and does increase the ratio of IgG2a to IgGl antibody.

Inventors: PENNY CHRISTOPHER L, PENNY, CHRISTOPHER, L.
Applicants: NORTH AMERICAN VACCINE INC, NORTH AMERICAN VACCINE, INC.
Application year: 1991
Main classification: A61K39
IPC codes: A61K39, C07K14