Non-invasive method for obtaining a material through the skin

Abstract: An in vitro method of diagnosis wherein a physiologic sample is assayed, the sample having been obtained at the skin surface by passage through the stratum corneum which has decreased barrier function as a result of exposure to ultrasound at a frequency of above about 10 MHz.

Description: This invention relates to the sampling of tissue beneath the skin or other body surface by application of high frequency (>10 MHz) ultrasound thereto. Skin is a structurally complex, relatively impermeable membrane. Molecules moving from the environment into and through intact skin must first penetrate the stratum corneum and any material on its surface. They must then penetrate the viable epidermis, the papillary dermis, and the capillary walls into the blood stream or lymph channels. To be so absorbed, molecules must overcome a different resistance to penetration in each type of tissue. Transport across the skin membrane is thus a complex phenomenon. However, it is the stratum corneum, a layer approximately 5-15 micrometers thick over most of the body, which presents the primary barrier to absorption of topical compositions or transdermally administered drugs. It is believed to be the high degree of kertainization within its cells as well as their dense packing and cementation by ordered, semicrystalline lipids which create in many cases a substantially impermeable barrier to drug penetration. Applicability of transdermal drug delivery is thus presently limited, because the skin is such an excellent barrier to the ingress of topically applied materials. For example, many of the new peptides and proteins now produced as a result of the biotechnology revolution cannot be delivered across the skin in sufficient quantities due to their naturally low rates of skin permeability. Various methods have been used to increase skin permeability, and in particular to increase the permeability of the stratum corneum. The primary focus has been on the use of chemical enhancers, i.e., wherein drug is coadministered with a penetration enhancing agent (or "permeation enhancer"). While such compounds are effective in increasing the rate at which drug is delivered through the skin, there are drawbacks with many permeation enhancers which limit their use. For example, many permeation enhancers are associated with deleterious effects on the skin (e.g. irritation). Iontophoresis has also been used to increase the permeability of skin to drugs, and involves (1) the application of an external electric field, and (2) topical delivery of an ionized form of drug (or of a neutral drug carried with the water flux associated with ion transport, i.e., via "electroosmosis"). While permeation enhancement via iontophoresis has, as with chemical enhancers, been effective, there are problems with the degree of irreversible skin damage induced by the transmembrane passage of current. The presently disclosed and claimed method involves the use of ultrasound to decrease the barrier function of the stratum corneum. "Ultrasound" is defined as mechanical pressure waves with frequencies above 20,000 Hz (see, e.g., H. Lutz et al., Manual of Ultrasound: 1. Basic Physical and Technical Principles (Berlin: Springer-Verlag, 1984)). As discussed by P. Tyle et al. in Pharmaceutical Research 6 (5): 355-361 (1989), drug penetration achieved via "sonophoresis" (the movement of drugs through skin under the influence of an ultrasonic perturbation; see D.M. Skauen and G.M. Zentner, Int. J. Pharmaceutics 20 : 235-245 (1984)), is believed to result from thermal, mechanical and chemical alteration of biological tissues by the applied ultrasonic waves. Unlike iontophoresis, the risk of skin damage appears to be low. Applications of ultrasound to drug delivery have been discussed in the literature. See, for example: P. Tyle et al., supra (which provides an overview of sonophoresis); S. Miyazaki et al., J. Pharm. Pharmacol. 40 : 716-717 (1988) (controlled release of insulin from a polymer implant using ultrasound); J. Kost et al., Proceed. Intern. Symp. Control. Rel. Bioact. Mater. 16 (141): 294-295 (1989) (overview of the effect of ultrasound on the permeability of human skin and synthetic membranes); H. Benson et al., Physical Therapy 69 (2): 113-118 (1989) (effect of ultrasound on the percutaneous absorption of benzydamine); E. Novak, Arch. Phys. Medicine & Rehab 45 : 231-232 (1964) (enhanced penetration of lidocaine through intact skin using ultrasound); J.E. Griffin et al., Amer. J. Phys. Medicine 44 (1): 20-25 (1965) (ultrasonic penetration of cortisol into pig tissue); J.E. Griffin et al., J. Amer. Phys. Therapy Assoc. 46 : 18-26 (1966) (overview of the use of ultrasonic energy in drug therapy); J.E. Griffin et al., Phys. Therapy 47 (7): 594-601 (1967) (ultrasonic penetration of hydrocortisone); J.E. Griffin et al., Phys. Therapy 48 (12): 1336-1344 (1968) (ultrasonic penetration of cortisol into pig tissue); J.E. Griffin et al., Amer. J. Phys. Medicine 51 (2): 62-72 (1972) (same); J.C. McElnay, Int. J. Pharmaceutics 40 : 105-110 (1987) (the effect of ultrasound on the percutaneous absorption of fluocinolone acetonide); and C. Escoffier et al., Bioeng. Skin 2: 87-94 (1986) (in vitro study of the velocity of ultrasound in skin). In addition to the aforementioned art, U.S. Patent Nos. 4,767,402 and 4,780,212 to Kost et al. relate specifically to the use of specific frequencies of ultrasound to enhance the rate of permeation of a drug through human skin or through a synthetic membrane. While the application of ultrasound in conjunction with drug delivery is thus known, results have for the most part been disappointing, i.e. enhancement of skin permeability has been relatively low. The present invention provides a novel method for enhancing the rate of permeation of a given material through a selected intact area of an individual's body surface. The method comprises contacting the selected intact area with the material and applying ultrasound to the contacted area. The ultrasound has a frequency of above about 10 MHz, and is continued at an intensity and for a period of time sufficient to enhance the rate of permeation of the material into and through the body surface. The ultrasound can also be used to pretreat the selected area of the body surface in preparation for diagnostic purposes, i.e., to enable non-invasive sampling of physiologic material beneath the skin or body surface. In addition to enhancing the rate of permeation of a material, the present invention involves increasing the permeability of a biological membrane such as the stratum corneum by applying ultrasound having a frequency of above about 10 MHz to the membrane at an intensity and for a period of time sufficient to give rise to increased permeability of the membrane. It is accordingly a primary object of the invention to address the aforementioned deficiencies of the prior art by providing a method of enhancing the permeability of biological membranes. It is another object of the invention to provide such a method which is effective with or without chemical permeation enhancers. It is a further object of the invention to enable sampling of tissue beneath the skin or other body surface by application of high frequency (>10 MHz) ultrasound thereto. A further feature of the invention is that it involves ultrasound of a frequency greater than about 10 MHz. Additional objects, advantages and novel features of the invention will be set forth in part in the description which follows, and in part will become apparent to those skilled in the art upon examination of the following, or may be learned by practice of the invention. Figures 1A, 1B and 1C are theoretical plots of energy dissipation within the skin barrier versus frequency of applied ultrasound. Figures 2, 3 and 4 are graphic representations of the amount of salicylic acid recovered from the stratum corneum after ultrasound treatment at different frequencies. Figures 5 and 6 represent the results of experiments similar to those summarized in Figures 2, 3 and 4, but with a shorter treatment time. Figures 7, 8, 9 and 10 are plots of enhancement versus "tape-strip number," as described in the Example. Figure 11 illustrates the effect of ultrasound on the systemic availability of salicylic acid following topical application. Before the present method of enhancing the rate of permeation of a material through a biological membrane and enhancing the permeability of membranes using ultrasound are disclosed and described, it is to be understood that this invention is not limited to the particular process steps and materials disclosed herein as such process steps and materials may, of course, vary. It is also to be understood that the terminology used herein is used for purpose of describing particular embodiments only and is not intended to be limiting since the scope of the present invention will be limited only by the appended claims. It must be noted that as used in this specification and the appended claims, the singular forms "a", "an" and "the" include plural reference unless the context clearly dictates otherwise. Thus, for example, reference to "a drug" includes mixtures of drugs and their pharmaceutically acceptable salts, reference to "an ultrasound device" includes one or more ultrasound devices of the type necessary for carrying out the present invention, and reference to "the method of administration" includes one or more different methods of administration known to those skilled in the art or which will become known to those skilled in the art upon reading this disclosure. In one aspect of the invention, a method is provided for enhancing the permeation of a given material through a biological membrane on an individual's body surface, which method comprises: (a) contacting the membrane with the chosen material in a pharmacologically acceptable carrier medium; and (b) applying ultrasound of an intensity and for a time effective to produce delivery of the material through the membrane. The ultrasound is of a frequency and intensity effective to increase the permeability of the selected area over that which would be obtained without ultrasound. The ultrasound has a frequency of more than 10 MHz, and may be applied either continuously or pulsed, preferably continuously. In an alternative embodiment, agents useful for diagnostic purposes may also be delivered through the body surface using the present method. The invention is useful as a non-invasive diagnostic technique, i.e., in enabling the sampling of physiologic material from beneath the skin or other body surface and into a collection (and/or evaluation) chamber. The present invention will employ, unless otherwise indicated, conventional pharmaceutical methodology and more specifically conventional methodology used in connection with transdermal delivery of pharmaceutically active compounds and enhancers. In describing the present invention, the following terminology will be used in accordance with the definitions set out below. A "biological membrane" is intended to mean a membrane material present within a living organism which separates one area of the organism from another and, more specifically, which separates the organism from its outer environment. Skin and mucous membranes are thus included. "Penetration enhancement" or "permeation enhancement" as used herein relates to an increase in the permeability of skin to a material such as a pharmacologically active agent, i.e., so as to increase the rate at which the material permeates into and through the skin. The present invention involves enhancement of permeation through the use of ultrasound, and, in particular, through the use of ultrasound having a frequency of greater than 10 MHz. While not wishing to be bound by theory, applicants propose that the use of higher frequency ultrasound as disclosed herein specifically enhances the permeation through the outer layer of skin, i.e., the stratum corneum, by causing momentary and reversible perturbations within (and thus short-term, reversible reduction in the barrier function of) the layer of the stratum corneum. As noted above, "ultrasound" is ultrasonic radiation of a frequency about 20,000 Hz. As may be deduced from the literature cited above, ultrasound used for most medical purposes typically employs frequencies ranging from 1.6 to about 10 MHz. The present invention, by contrast, employs ultrasound frequencies of greater than about 10 MHz, preferably in the range of about 15 to 50 MHz, most preferably in the range of about 15 to 25 MHz. It should be emphasized that these ranges are intended to be merely illustrative of the preferred embodiment; in some cases higher or lower frequencies may be used. The ultrasound may be pulsed or continuous, but is preferably continuous when lower frequencies are used. At very high frequencies, pulsed application will generally be preferred so as to enable dissipation of generated heat. The preferred intensity of the applied ultrasound is less than about 5.0 W/cm 2 , more preferably is in the range of about 0.01 to 5.0 W/cm 2 , and most preferably is in the range of 0.05 to 3.0 W/cm 2 . Virtually any type of device may be used to administer the ultrasound, providing that the device is capable of producing the higher frequency ultrasonic waves required by the present method. As ultrasound does not transmit well in air, a liquid medium is generally needed to efficiently and rapidly transmit ultrasound between the ultrasound applicator and the skin. As explained by P. Tyle et al., cited above, the selected drug medium should contain a "coupling" or "contacting" agent typically used in conjunction with ultrasound. The coupling agent should have an absorption coefficient similar to that of water, and furthermore be nonstaining, nonirritating to the skin, and slow drying. It is clearly preferred that the coupling agent retain a paste or gel consistency during the time period of ultrasound administration so that contact is maintained between the ultrasound source and the skin. Examples of preferred coupling agents are mixtures of mineral oil and glycerine and propylene glycol, oil/water emulsions, and a water-based gel. A solid-state, non-crystalline polymeric film having the above-mentioned characteristics may also be used. With respect to skin location, virtually any area of the body surface may be selected so long as it is intact, however, the thickness and permeability of skin at the site of exposure will affect the treatment conditions, i.e., intensity, frequency, contact time, exposure time, and the like. The area of skin through which the drug medium and the ultrasound will be administered can again vary greatly, but will typically be on the order of 1 to 100 cm 2 , more typically on the order of 5 to 100 cm 2 , most typically on the order of 10 to 50 cm 2 . Example [This example is provided for general guidance in working the present invention but relates to drug delivery by increasing the permeability of the skin.] Based on the present inventors' theoretical analysis of the propagation of ultrasound energy in tissue and the barrier properties of skin, it was concluded that higher ultrasound frequencies might be more effective in enhancing the flux of drug molecules across the skin. This conclusion, based on the assumption that enhancement is proportional to the amount of energy dissipation within the barrier, is supported by Figure 1, which suggests that, within the first millimeter of skin, ultrasound energy dissipation increases exponentially with frequency. To test the hypothesis that higher frequencies (>10 MHz) would yield greater enhancement, in vivo experiments were performed on hairless guinea pigs. The setup for carrying out the experiment consisted of a function generator, transducers tuned at different frequencies and a power-meter. 14 C-labeled salicylic acid was used as the "model" marker drug molecule. A saturated solution of unlabeled salicylic acid in water was prepared. Carbopol™ (B.F. Goodrich), a polymer, was added to this solution to make a gel containing salicylic acid at a concentration of 0.57% w/w. This gel was then spiked with a known amount of radiolabeled salicylic acid (approx. 2.27 µCi/mg gel). Approximately 30 mg gel per square centimeter of transducer cross-sectional area was then applied to the skin surface of the guinea pigs' flanks. This gel served as both the drug reservoir and the coupling medium between the transducer and skin surface. Frequencies of 1, 7.5 and 16 MHz were tested using an intensity of 0.25 W/cm 2 and treatment periods (time of exposure of the skin to both drug and ultrasound) of 10 and 20 minutes. At the treatment site, the transducer delivered ultrasound at the appropriate frequency for the designated period. At the control site, in the contralateral flank, the transducer was positioned on the skin, but not activated. Thus, each animal served as its own control. Enhancement was quantified in two ways: (1) by tape-stripping the outer skin layer at the treatment and control sites immediately after the experimental period (radioactivity in the tape strips was then determined by liquid scintillation counting); and (2) by measuring the cumulative amount of 14 C excreted in the animals' urine up to 14 hours after initiation of the experiment. (1) Tape-strip procedure: It has been established that the uppermost layer of skin, the stratum corneum (SC), offers the most resistance to drug penetration. Hence, it was decided to compare the amount of radioactivity present in the SC after ultrasound treatment with that after the control experiment (passive diffusion, no ultrasound). Figures 2, 3 and 4 present a comparison of the total amount of salicylic acid that had penetrated into the SC with 1, 7.5 and 16 MHz exposure for 20 minutes and without exposure to ultrasound. As can be seen, use of 16 MHz resulted in a significantly elevated drug level in the SC as compared to the control. Figures 5 and 6 are comparable to Figures 3 and 4, but here the treatment time was 10 minutes, rather than 20 minutes. Each tape strip removed a certain amount of the SC. Hence, with increasing tape-strip number, tissue further away from the surface was examined. Therefore, a plot of the amount of drug in each strip against strip number reflects the concentration gradient of drug in the SC. Such plots are shown in Figures 7, 8, 9 and 10. The ordinate is the ratio of the amount of radioactivity in the tape-strip after treatment to the amount in the strip after control. (2) Urinary excretion: To confirm that the amount of drug recovered from the SC reflected the amount of drug penetrated, radioactivity excreted in the urine was monitored. Figure 11 graphically illustrates a comparison of the total amount of radioactivity excreted in urine 14 hours after a 20 minute treatment using 16 MHz and the corresponding control. At least five times more drug entered the systemic circulation with ultrasound than without. While the present invention has been described with reference to specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit of the scope of the invention. In addition, many modifications may be made to adapt a particular ultrasound device, drug, process, process step or steps, to the objective scope of the present invention. All such modifications are intended to be within the scope of the claims appended hereto.

Claims: A non-invasive method for obtaining a physiologic sample for assay, wherein the sample is obtained at the skin surface by passage through the stratum corneum which has decreased barrier function as a result of exposure to ultrasound at a frequency of above about 10 MHz. A method according to claim 1 wherein the exposure to ultrasound has occurred for between about from 30 seconds to 60 minutes. A method according to claim 2 wherein the exposure is from 5 to 45 minutes. A method according to any one of claims 1 to 3 wherein the method additionally comprises the use of a coupling compound effective to enhance the transfer of ultrasound to a biological membrane from an ultrasound source. A method according to claim 4 wherein the coupling compound is a polymer or gel. A method according to claim 5 wherein the coupling compound is a mineral oil, glycerine, water or propylene glycol. A method according to any one of the preceding claims wherein the exposure to ultrasound is at a frequency of from about 15 MHz to 50 MHz. A method according to any one of the preceding claims wherein the intensity of the ultrasound is less than about 5.0 W/cm 2 . A method according to claim 8 wherein the intensity of the ultrasound is from about 0.01 to 5.0 W/cm 2 .

Inventors: BOMMANNAN DURAIRAJ, FLYNN GORDON L, GUY RICHARD HENRY, OKUYAMA HIROSHIA, STAUFFER PAUL, BOMMANNAN, DURAIRAJ, FLYNN, GORDON L., GUY, RICHARD HENRY, OKUYAMA, HIROSHIA, STAUFFER, PAUL
Applicants: CYGNUS THERAPEUTIC SYSTEMS, UNIV CALIFORNIA, CYGNUS, INC., THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Application year: 1991
Main classification: A61M37
IPC codes: A61B10, A61B17, A61N7, A61K9, A61M37