FLY ATTRACTANT

Abstract: [Constitution]: Fly attractant containing as the active ingredient an extract prepared by extracting bamboo with a solvent or squeezing bamboo. [Effect]: This attractant has an excellent effect of attracting flies and is used for controlling them.

Description: This invention relates to a fly attractant containing as an active ingredient a composition which is extracted from a bamboo with an extractive solvent or an extract which is squeezed from a bamboo. Extracts from bamboo are known to have useful properties: Jp 2-180804 describes the use of such an extract as an anti-bacterial agent. Flies breed in large flocks in poultry houses, pig houses, cow houses and refuse disposal stations, and they not only affect the working efficiency at the spots of breeding but also are becoming a public nuisance in the residential area in the neighborhood. Pyrethroids, organophosphorus and carbamates are widely used for the control of the adult and larval forms of these flies, and insect growth regulators are widely used for larval control. However, the efficacy of such methods has recently been notably reduced due to the development of resistance by the flies to these agents and to the development of large-scale facilities. Particularly, control of Fannia Spp . in deep-bed poultry houses is extremely difficult. The reasons are that the larvae of Fannia are not very sensitive to various chemicals and that it is not only difficult to spray the breeding spots, which are located just under the cages, but also the chemical solutions do not fully penetrate into the manure. Consequently, adult control is widely employed for the Fannia . However, adult control is not fully effective, as it sometimes only causes the flies to leave the poultry houses. Meanwhile, a Paint On Baits method (attractant-incorporated insecticide for use in the residual spray method) has been proposed as a spray insecticide for use on ceilings where the adults of Fannia rest during the night, which comprises a syrup or sugar having an attracting action. This method is utilized to increase the efficiency (e.g. "Chikusan no Kenkyu (Study on Livestock Industry)", 42 , 745-748, 846-850 (1988); "Seikatsu to Kankyo (Living and Environment)", 36 (No. 8), 106-113 (1991)). The attractants disclosed in these articles failed to yield satisfactory results, as they exhibit only a small attraction of the flies to the sprayed spots, although they may show an increased effectiveness when the flies happen to rest on the sprayed surface. There are also various kinds of fly catching techniques known using, for example, a fly attracting trap, a fly ribbon and flypaper. However, few of the attractants used in these methods, such as syrups and sweeteners, can yield satisfactory results. The present inventors studied intensively for years to accomplish this invention and found that a composition extracted from a bamboo with an extractive solvent or an extract squeezed from a bamboo can be an excellent attractant for flies and that it can be an excellent novel attractant when used for fly control and can be used in the attractant-incorporated insecticide, via the residual spray method, or in a fly attracting trap and the like. The present invention provides the use of a composition extracted from a bamboo using an extractive solvent or an extract squeezed from a bamboo as a fly attractant. The bamboo employable according to the present invention can be those belonging to Gramineae or Bambusoides, including, for example, Phyllostachys heterocycla , Phyllostachys nigra , Phyllostachys bambusoides and ground bamboo (particularly a shoot thereof), preferably a shoot of Phyllostachys heterocycla and Phyllostachys bambusoides . The solvent employable for the extraction from a bamboo includes hydrocarbons such as hexane, benzene, toluene and xylene; ethers such as diethyl ether, tetrahydrofuran, dioxane and 1,2-dimethoxyethane; esters such as methyl acetate and ethyl acetate; alcohols such as methanol, ethanol, propanol and butanol; halogenated hydrocarbons such as methylene chloride, chloroform and carbon tetrachloride; amides such as dimethylformamide and dimethylacetamide; nitriles such as acetonitrile; sulfoxides such as dimethyl sulfoxide; and water or mixtures of such organic solvents with water, preferably ethers, aqueous ethers, alcohols, aqueous alcohols and water, more preferably alcohols and water, and particularly preferably methanol and ethanol. The species of fly to be attracted is not particularly limited so long as it belongs to Muscidae or Muscinae (Diptera) and includes, for example, Musca domestica , Fannia canicularis and Muscina stabulans . The composition extracted from a bamboo of the present invention can readily be prepared in the following manner. For example, a bamboo (preferably a bamboo shoot whose sheath is removed) is finely chopped, an extractive solvent is added thereto to carry out extraction at 0 to 200°C (preferably at room temperature to 150°C) for 30 minutes to 48 hours (preferably 1 to 24 hours) and then the extract solution is concentrated, or the extractive solvent is evaporated from the extract solution, to obtain the composition. Otherwise, the bamboo extract can be obtained by finely chopping or compressing a bamboo (preferably a bamboo shoot whose sheath is removed) to squeeze out a juice therefrom. The composition extracted from a bamboo with an extractive solvent or extract squeezed from a bamboo shows an excellent fly attracting activity and can be used for control of flies as an attractant-incorporated insecticide in the residual spray method or as a fly attracting trap and the like. The activity of a composition extracted from a bamboo shoot with an extractive solvent as a fly attractant was tested, as described below. (Test Example 1) Fly attracting activity (1) Preparation of attractant composition Attractant composition was prepared by mixing 10 ml (active ingredient: 100mg) of a 1 % solution of Azamethiophos (insecticide), 10 g of a sugar and 10 ml of each extract solution obtained in Preparation Examples 1 to 3. (2) Effectiveness of attracting flies 1 ml of the attractant composition was dropped on to filter papers No. 2 having a diameter of 9 cm, and the thus treated filter papers were placed on dishes having a diameter of 9 cm and dried. These dishes were arranged on tables in a poultry farm at 30 cm intervals. After 60 minutes, the number of flies attracted to the dishes were recorded. The results are shown in Table 1. No. Bamboo Shoot Extract Number of flies attracted 1 Extract with Ethanol 45 2 Extract with Methanol 29 3 Extract with Water 16 4 None (Mixture of an insecticide and sugar) 3 As demonstrated by Test Example 1, the bamboo shoot extracts showed excellent activity in attracting various kinds of flies. As described above, a composition extracted from a bamboo using an extractive solvent or an extract squeezed from a bamboo can show excellent activity in attracting flies and can be ulitized for fly control as an attractant-incorporated insecticide in the residual spray method or as a fly attracting trap and the like. The composition or extract of the invention is preferably used in an appropriate dosage form for insecticidal preparations or incorporated into a system by methods well known in the art. For example, when the composition or extract from a bamboo is employed in an attractant-incorporated insecticide in the residual spray method, a wettable power or an emulsifiable concentrate of organophosphate insecticides such as propentaphos, azamethiophos, chloropyriphosmethyl, diazino, trichlorfone and phenitrothion; pyrethroids such as allethrin, permethrin, chrysron and ethophenprox; and carbamate insecticides such as carbaryl, propuxur and 2-s-butylphenyl N-methylcarbamate (BPMC) is diluted arbitrarily with water, and then the composition or extract of the present invention is added thereto to provide a spray. The composition of the invention (extract solution) is used in an amount of 0.01 to 10 w/w % (preferably 0.05 to 1 w/w %) based on the diluted insecticide solution (containing about 0.2 % by weight of active insecticidal ingredient). Meanwhile, when the composition or extract from a bamboo is used in a fly attracting trap, it is preferably prepared in an appropriate dosage form using adjuvants. The adjuvants include, for example, alcohols such as methanol, ethanol, propanol, butanol and octanol, fuel oils, machine oils, paper, sugar, milk, non-fat dry milk, rice bran, wheat bran, corn power, flour and livestock feed. The composition or extract of the invention is dissolved in such an adjuvant, or such an adjuvant is impregnated with the composition or extract of the invention to provide a suitable dosage form, which is then set in a trap having an appropriate design. The composition of the present invention (extract solution) is used in an amount of 0.05 to 10 (preferably 0.1 to 2) based on the dilute insecticide solution (containing about 0.1 % by weight of the active insecticidal ingredient) in terms of weight ratio. Meanwhile, heat, wind, etc. can be utilized for diffusing the attractant ingredient, whereas light or sex pheromone can additionally be used for enhancing the effectiveness of attracting flies. Further, when the composition or extract of the invention is used in fly ribbons or fly papers, it is admixed with a sticky substance such as a mixture of gum arabic, vinyl chloride emulsion and castor oil, and then, for example, papers boards, synthethic resin plates, plywoods or fiber ropes are immersed in the resulting mixture, or the resulting mixture is sprayed onto such a base to provide fly ribbons and the like. The attractant composition can also be sprayed onto commerically available fly ribbons. (Brief Description of the Drawings) Fig. 1 shows a UV spectrum of the methanol extract solution; Fig. 2 shows a high-performance liquid chromatogram of the methanol extract solution; Fig. 3 shows a UV spectrum of the ethanol extract solution; Fig. 4 shows a high-performance liquid chromatogram of the ethanol extract solution; Fig. 5 shows a UV spectrum of the aqueous extract solution; Fig. 6 shows a high-performance liquid chromatogram of the aqueous extract solution; Fig. 7 shows silica gel thin layer chromatograms of the ethanol extract solution, methanol extract solution and aqueous extract solution staining with ninhydrin; and Fig. 8 shows silica gel thin layer chromatograms of the ethanol extract solution, methanol extract solution and aqueous extract solution staining with p-anisaldehyde. Next, the present invention will be described more specifically be way of preparation Examples and Formulation Examples; (Preparation Example 1) Preparation of methanol extract solution 250 g of shoots of Phyllostachys bambusoides were finely chopped, 500 ml of methanol was added thereto and then the mixture was refluxed with heating for 2 hours. After cooling of the mixture, the insolubles were filtered off and the filtrate was concentrated to 200 ml under reduced pressure. The concentrate was washed with 200 ml of hexane and further concentrated to 100 ml under reduced pressure to obtain a methanol extract solution. A UV spectrum of a sample prepared by dissolving 1 mg of the thus obtained extract solution in 10 ml of a 50 % aqueous ethanol is shown in Fig. 1. Meanwhile, a high-performance liquid chromatogram (column: Inertsil ODS-2, manufactured by G L Science; eluent: water/acetonitrile = 20/80; flow rate: 1 ml/min; detector: 200 nm UV detector) of this extract solution is shown in Fig. 2. The numerals in Fig. 2 are the retention time (minute). (Preparation Example 2) Preparation of ethanol extract solution 250 g of shoots of Phyllostachys bambusoides were finely chopped, 500 ml of ethanol was added thereto and then the mixture was allowed to stand at room temperature overnight. After the insolubles had been filtered off, the filtrate was concentrated to 100 ml under reduced pressure to obtain an ethanol extract solution. A UV spectrum of a sample prepared by dissolving 1 mg of the thus obtained extract solution in 10 ml of a 50 % aqueous ethanol is shown in Fig. 3. A high-performance liquid chromatogram (under the same measurement conditions as in the case of methanol extract solution) of this extract solution is shown in Fig. 4. The numerals in Fig. 4 are the retention time (minutes). (Preparation Example 3) Preparation of aqueous extract solution 250 g of shoots of Phyllostachys bambusoides were finely chopped, 500 ml of a distilled water were added thereto and then the mixture was heated until about half of the water has been distilled off (about 2 hours). After cooling of the residue, the insolubles therein were filtered off to obtain an aqueous extract solution. A UV spectrum of a sample prepared by dissolving 1 mg of the thus obtained extract solution in 10 ml of 50 % aqueous ethanol is shown in Fig. 5. A high-performance liquid chromatogram (under the same measurement conditions as in the case of methanol extract solution) of this extract solution is shown in Fig. 6. The numerals in Fig. 6 are the retention time (minutes). Further, silica gel thin-layer chromatograms (developing solvent: butanol/acetic acid/water = 5/1/1) of the extract solutions obtained in Preparation Examples 1 to 3 are shown in Fig. 7 (staining with ninhydrin) and in Fig. 8 (staining with p-anisaldehyde), respectively. In Fig. 7, the numerals 1, 4 and 7 are chromatograms of ethanol extract solutions having different concentrations, 2, 5 and 8 are those of methanol extract solutions having different concentrations and 3, 6 and 9 are those of aqueous extract solutions having different concentrations; while in Fig. 8, the numerals 1 and 4 are chromatograms of ethanol extract solutions having different concentrations, 2 and 5 are those of methanol extract solutions having different concentrations and 3 and 6 are those of aqueous extract solutions having different concentrations. The dotted spots in Figs. 7 and 8 are those having UV absorption band (254 nm). (Formulation Example 1) Spray 100 g of a wettable powder containing 50 % of Propentaphos manufactured by Sankyo Co., Ltd. were diluted 300-fold with water, and 75 g of the methanol extract solution obtained in Preparation Example 1 were added thereto and dispersed homogeneously therein to provide a spray containing 0.25 % by weight of the attractant extract solution. (Formulation Example 2) Fly ribbon 100 g of a sticky substance which is a mixture of gum arabic, a vinyl chloride emulsion and caster oil (viscosity controller) were mixed with 10 g of the methanol extract solution obtained in Preparation Example 1, and the resulting mixture was sprayed onto each surface of a paper board (7 cm x 70 cm) in an amount of 2 g in total to provide a fly ribbon. (Formulation Example 3) Dry bait for fly attracting trap 50 g of the methanol extract solution obtained in Preparation Example 1 were added to 100 g of a 10 % aqueous solution of a insecticide Azamethiophos diluted 100-fold with water and then 500 g of brown sugar and 500 g of rice bran were added thereto to provide a dry bait for a fly attracting trap. (Formulation Example 4) Dry bait for fly attracting trap 50 g of the ethanol extract solution obtained in Preparation Example 2 were added to 100 g of a 10 % aqueous solution of an insecticide Azamethiophos diluted 100-fold with water and then 500 g of brown sugar and 500 g of rice bran were added thereto to provide a dry bait for a fly attracting trap.

Claims: The use of a composition extracted from a bamboo using an extractive solvent, or an extract squeezed from a bamboo as a fly attractant. The use of Claim 1, wherein the fly attractant is a composition extracted from a bamboo using an extractive solvent. The use of Claim 2, wherein said bamboo is a bamboo shoot. The use of Claim 2, wherein said solvent is water or an alcohol. The use of Claim 2, wherein said solvent is methanol or ethanol. The use of Claim 2, wherein said bamboo is a bamboo shoot and said solvent is water or an alcohol. The use of Claim 2, wherein said bamboo is a bamboo shoot and said solvent is methanol or ethanol. The use of Claim 1, wherein the attractant is formulated as an attractant-incorporated insecticide for use in the residual spray method. The use of Claim 8, wherein the attractant is used in an amount of 0.01 to 10 w/w % based on said insecticide containing 0.2 % by weight of an active insecticidal ingredient. The use of Claim 1, wherein the attractant is formulated for use as a fly attracting trap. The use of Claim 10, wherein the attractant is used in an amount of 0.05 to 10 based on said insecticide containing 0.1 % by weight of an active insecticidal ingredient in terms of weight ratio.

Inventors: SAITO AKIO SANKYO COMPANY LIMI, TABARU YUICHIRO SANKYO COMPANY, SAITO, AKIO SANKYO COMPANY, LIMITED, TABARU, YUICHIRO SANKYO COMPANY, LIMITED
Applicants: SANKYO CO, SANKYO COMPANY LIMITED
Application year: 1991
Main classification: A01N65
IPC codes: A01P19, A01M1, A01N65, A01N25