Novel N-acyldipeptides, and pharmaceutical compositions containing the same

Abstract: Novel N-acyldipeptides of formula I wherein R represents a rest of formula and R₄, Y, m, n and Z have the meaning as defined in the description, R₁ represents hydrogen, a 1-10C alkyl, an optionally substituted methyl or benzyl, R₂ represents a-CO-A group, wherein A has the meanings as defined in the description, R₃ represents a -(CH₂) p -CO-W group, wherein p and W have the meaning as defined in the description, are obtained by reacting a) a carboxylic acid II R-COOH II with a dipeptide III b) an acid chloride IV R - CO - Cl IV with a dipeptide III c) a carboxylic acid II R-COOH II with an amino acid V to a compound VI which is reacted with an amino acid VII d) an acid chloride IV R - CO - Cl IV with an amino acid V to a compound VI which is reacted with an amino acid VII wherein the substituents , R, R₁, R₂ and R₃ have the meaning as defined in the description. Compounds of formula I possess immunomodulatory and antitumoral activities.

Description: The invention belongs to the field of pharmaceutical industry and concerns novel N-acyldipeptides and pharmaceutical compositions containing the same. The novel N-acyldipeptides possess immunomodulatory and antitumoral activities. There exists a continuing need for novel medicaments having strong immunomodulatory and antitumoral activities, a selective effect upon the immunological system and as few side effects as possible. Recently, peptides having biological activity have been acquiring increasing significance in this field. Muramyl peptides [A. Adam and E. Lederer in Med. Res. Rev. (1984), 4, 111; G. Baschang in Tetrahedron (1989), 22, 6331] are components of microbial cell walls having therapeutically interesting effects upon the immunological system. The synthesis thereof, however, is a very exacting, multi-stage and expensive one [P. Lefrancier and E. Lederer in Fortschr. Chemie Org. Naturstoffe (1981), 40, 1]. N-acetylmuramyl-L-alanyl-D-isoglutamine (muramyl dipeptide, MDP) is the smallest essential structural element of bacterial cell wall having immunomodulatory activity. However, MDP also has several side effects, e.g. a pronounced pyrogenous and somnogenous activity, and its can also cause acute arthritis and anaphylactic reaction. Japanese authors [Y. Kitaura, H. Takeno, S. Okada, S. Yanischi, K. Hemmi, J. Mori, H. Senah, J. Mine, M.Hashimoto in J. Med. Chem. (1982), 25, 335] have isolated and synthetized various tetrapeptides [lactyl-L-alanyl-D-isoglutamyl-D,L-diaminopimelyl glycine and N-lauroyl-L-alanyl-D-isoglutamyl-L,L-diaminopimelyl glycine] having immunomodulatory properties similar to those of MDP. Austrian researchers [H. Hönig and R. Zenk in Chem. Phys. Lipids (1990), 53, 347] have described the synthesis of N-(11-metacroylamino-undecanoyl)-L-alanyl-D-isoglutamine and 2-(2-(11-metacroylamino-undecanoyl)aminoethoxy)propanoyl-L-alanyl-D-isoglutamine as a monomer for the polymeric vesicles having the partial MDP structure. Further, there has been published the synthesis of acyclic MDP analogues [J. Danklmaier and H. Hönig in Liebigs Ann. Chem. (1990), 145], where the authors focused on the synthesis of N-(2-(2-(acylamino)-1-methyl-ethoxy)propanoyl)-L-alanyl-D-isoglutamine compounds having immunomodulatory activity. GB-A-2 053 232 shows similar compounds which, however, essentially differ in the structure from the compounds according to the present invention, and Proc. Europ. Pept. Symp. 21th (1990) shows compounds wherein the structure is tripeptide Gly-L-Ala-D-iGln, and glycine is substituted by a 1- or 2-adamantyl residue, and no carbonyl group is present at the adamantane moiety. BBRC 72 (1976), pages 339-349, describes a specific N-acetyl-muramyl derivate, wherein β-elimination leads to a corresponding lactyl-dipeptide derivative. The solution to the above outlined problem underlying the present invention is achieved by providing novel N-acyldipeptides which are N-(7-oxooctanoyl)-L-alanyl-D-isoglutamine, N-(7-oxododecanoyl)-L-alanyl-D-isoglutamine, N-(5-phthalimidopentanoyl)-L-alanyl-D-isoglutamine, N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-glutamic acid and (N-(2-(2-(1-adamantanecarboxamido)-ethoxy)-acetyl)-L-alanyl-D-isoglutamine. Further, there are provided pharmaceutical compositions containing as the active ingredient a therapeutically effective amount of the aforementioned N-acyldipeptides together with common pharmaceutically acceptable carriers and excipients. The starting compounds for the N-Acyldipeptides according to the invention are prepared, unless stated otherwise, according to the methods described in the literature as described by S. Hünig, E. Lücke and E. Benzing in Chem. Ber (1958), 91, 129; F. P. Prout and J. Cason in J. Org. Chem. (1949), 14, 132; G. Nefkens, G. Tesser and R. Nivard in Recueil (1960), 79, 249; C. R. McArthur, P. M. Worster, J. L. Jiang and C. C. Lenznoff in Can. J. Chem. (1982), 60, 1836; L-alanyl-D-isoglutamine γ-benzyl ester hydrochloride is prepared as described by P. Lefrancier and E. Bricas in Bull. Soc. Chim. Biol. (1967), 49, 1257. BIOLOGICAL TESTS The following tests were performed: 1. Immunological tests 1.1 Determination of the number of lymphocytes B and lymphocytes T 1.2 Determination of the number of macrophages 1.3 Blast transformation of lymphocytes by mithogens 1.4 Activation of peritoneal macrophages 1.5 Determination of haemolytic plaques for the assessment of the maturation of lymphocytes B 2. Antitumour activity 3. Pyrogenous activity 4. Toxicity 1. Immunological tests Immunological studies were performed on female HAN-NmRI strain mice of 3-4 weeks, weighing from 18-21 g. In tests 1.1 to 1.5, the animals were first administered the Brewer's thioglycolate medium by i.p. injection, which was followed after 6 hours by the injection of 0.5 ml of a solution containing 25 µ g, 2.5 µ g and 0.25 µ g respectively of N-acetyl-muramyl-L-alanyl-D-isoglutamine (muramyl dipeptide, MDP) or of N-(7-oxooctanoyl)-L-alanyl-D-isoglutamine (compound 1, Example 19). Equal doses were administered to the animals on days 2 and 3. On day 4 the animals were sacrificed and the spleen and the peritoneal macrophages were isolated. 1.1 Determination of the number of lymphocytes B and lymphocytes T To the isolated splenic lymphocytes there were added fluorescein isothiocyanate labelled antimouse antibodies (for the determination of lymphocytes B) and anti-Thy 1 monoclonal antibodies (for the determination of lymphocytes T) respectively. After incubation and rinsing with RPMI 1640 medium (Gibco, Great Britain), the lymphocytes were counted by fluorescence microscopy. Neither the number of splenic lymphocites B nor of lymphocytes T was altered to a statistically significant extent either by muramyl dipeptide or by compound 1. 1.2 Determination of the number of macrophages The number of macrophages in the peritoneal cavity washings was determined three days after the stimulation with Brewer's thioglycolate medium. The cells were suspended in ice-cold RPMI 1640 medium, to which trypan blue was added, and counted in Neubauer's chamber. The number of peritoneal macrophages was not altered to a statistically significant extent either by muramyl dipeptide or by compound 1. 1.3 Blast transformation of lymphocytes by mithogens Isolated splenic lymphocytes were prepared in a concentration of 1 x 10 6 /ml in the RPMI 1640 medium, to which there were added 10% of foetal calf serum (FCS) (Sera-Lab, Great Britain), 1 ml of 200 mM L-glutamine solution and 1 ml of an antibiotic solution (100 U/ml of penicillin and 100 µ g/ml of streptomycin) for 100 ml of complete medium. To each flat bottom well of a microtitration plate (T grade, Nunc, Denmark) 100 µl of the cell suspension were distributed. The cells were stimulated in vitro by adding concanavalin A (con A) (Pharmacia, Sweden) at respective concentrations of 16, 8, and 4 µ g/ml. Control lymphocyte cultures were grown in basal medium only. The cells were then incubated at 37 °C, 5% CO 2 and 95% humidity for 2 days, followed by the addition of tritium labelled thymidine. After 16 hours the samples were prepared for the measurement in a β-counter. The results were expressed as incorporation indexes with respect to the control group of the animals. Studies of concanavalin A influence on the blast transformation ability showed that muramyl dipeptide did not affect the blastic activity of lymphocytes T, whereas the compound 1 in the dose of 25 µ g increased this activity statistically significantly for about 70% (p < 0.05). In vivo immunomodulatory effect of muramyl dipeptide (MDP) and of compound 1 on the blast transformation ability of lymphocytes, stimulated in vitro with concanavalin A Substance Daily dose ( µ g) Amount of active thymidine incoporated in lymphocyte DNA (cpm) x ± s.e.m (n) p MDP 25 7460 ± 1980 (5) >0.05 2.5 12440 ± 3540 (5) >0.05 0.25 19840 ± 9400 (5) >0.05 Compound 1 25 16380 ± 2950 (5) <0.05 2.5 19080 ± 5470 (5) >0.05 0.25 16860 ± 5210 (5) >0.05 Control 10130 ± 476 (5) --- When compared with MDP, compound 1 showed a statistically greater (p < 0.05) immunomodulatory effect in lymphocytes T at the dose of 25 µ g. 1.4 Activation of peritoneal macrophages The peritoneal cavity of the animals was rinsed with 4 ml of ice-cold RPMI 1640 medium. The cytolysis of erythrocytes was effected with 0.2% NaCl. The remaining cells were washed twice in a cooled centrifuge at 4 °C and 1500 rpm for 5 minutes with ice-cold RPMI 1640 medium. The cells were re-suspended and the concentration was adjusted to 1.5 x 10 6 /ml. The cell suspension was distributed among the flat bottom wells of a microtitration plate (T grade, Nunc, Denmark). After incubating for 2 hours at 37 °C, 5% CO 2 and 95% humidity, the wells were rinsed with warm RPMI 1640 medium and the adhering macrophages were used for testing. The macrophages were covered with 100 µl of a 160 µM ferricytochrome C solution in HBSS (Hank's Balanced Salt Solution) without phenol red or with 100 µl of ferricytochrome C in a 200 nM phorbol miristate acetate (PMA, Sigma, St. Louis, USA) solution or with 100 µl of ferricytochrome C, PMA and 340 units/ml of superoxide dismutase (SOD, Sigma, St. Louis, USA), which specifically inhibits the reduction of cytochrome C with the superoxide ion (blank) respectively. After incubating for 90 minutes at 37 °C, 5% CO 2 and 95% humidity, the absorbance was measured at the wavelength of 570 nm. The results are shown in Table 2 as the difference in the absorbance of the test sample and of the blank, calculated for 1 mg of cell protein. In the studies of macrophage activation it was shown that muramyl dipeptide increased the activity at all three doses by about 120% (p < 0.01), whereas the compound 1 at the dose of 0.25 µg did not increase the activity, whereas at the dose of 2.5 and 25 µ g the activity was increased by 40% (p < 0.05) and 90% (p < 0.001) respetively. For the compound 1 the effects were dose-dependent. As shown in Table 3, after additional macrophage activation with PMA, the activity was increased in both cases at all three doses. In the case of muramyl dipeptide the activity was most increased at the lowest dose, i.e. by about 160% (p < 0.01), whereas at both other doses the activity was only increased by about 115% (p < 0.001). With compound 1 the activity increased with increasing doses. At the dose of 0.25 µg the effect was increased by about 50% (p > 0.05), at the dose of 2.5 µg by about 60% (p < 0.05) and at the dose of 25 µ g by about 120% (p < 0.001) respectively. In either test at the doses of 2.5 and 25 µ g there was no statistically significant difference (p > 0.05) between the respective macrophage activation achieved by muramyl dipeptide and by compound 1. In vivo immunomodulatory effect of muramyl dipeptide (MDP) and of compound 1 upon macrophage activation without additional in vitro stimulation with phorbol miristate acetate (PMA) Substance Daily dose ( µ g)* Difference in absorbancies of the test sample and of the blank per mg of cell protein x ± s.e.m (n) p MDP 25 578 ± 81 (5) <0.01 2.5 578 ± 42 (5) <0.001 0.25 614 ± 35 (5) <0.001 Compound 1 25 518 ± 20 (5) <0.05 2.5 387 ± 56 (5) <0.05 0.25 273 ± 40 (5) >0.05 Control 275 ± 26 (5) --- In vivo immunomodulatory effect of muramyl dipeptide (MDP) and of compound 1 upon macrophage activation with additional in vitro stimulation with phorbol miristate acetate (PMA) Substance Daily dose (µg) Difference in absorbancies of the test sample and of the blank per mg of cell protein x ± s.e.m (n) p MDP 25 2138 ± 165 (5) <0.001 2.5 2207 ± 213 (5) <0.001 0.25 2657 ± 595 (5) <0.05 Compound 1 25 2200 ± 177 (5) <0.001 2.5 1630 ± 297 (5) <0.05 0.25 1480 ± 336 (5) >0.05 Control 1004 ± 38 (5) --- 1.5 Determination of haemolytic plaques for the assessment of the maturation of lymphocytes B A 1% suspension of sheep erythrocytes (Institute of Microbiology, Faculty of Medicine, Ljubljana, Yugoslavia) in physiological saline was used for the immunisation. First, individual mice were administered 0.2 ml of this suspension by i.p. injection, followed next day by 0.1 ml (1 µ g/mouse) of the test substance. The immunisation was completed on the fifth day after the administration of sheep erythrocytes and the mice were sacrificed. Their spleens were removed and homogenized in Parker 199 Medium with added aminoacids, streptomycin and sodium carbonate (Torlak, Beograd, Yugoslavia). The lymphocytes were separated on the Ficol Separating Medium (Pharmacia, Uppsala, Sweden). After repeated rinsing with Parker 199 Medium, the cells were re-suspended in RPMI 1640 nutrient medium with added 10% of foetal calf serum and streptomycin. To 50 µl of the cell suspension, 450 µl of trypan blue were added and the cells were counted in Neubauer's chamber. The number of lymphocytes per ml of the cell suspension was calculated. To 100 µ l of the diluted cell suspension there were added 200 µl of RPMI 1640 nutrient medium, 50 µl of a 10% suspension of sheep erythrocytes and 50 µl of guinea pig complement (Institute of Microbiology, Faculty of Medicine, Ljubljana, Yugoslavia). The reaction mixture (RM) was put into the prepared chambers on the slide, the chambers were sealed with white wax and incubated at 37 °C for 60 minutes. After the completion of the incubation, the plaques were counted under microscope. The number of plaques per 1 x 10 6 cells was calculated according to the following equations: Number of plaques = 1 x 10 6 cells x number of plaques per chamber A cells per chamber A cells per chamber = amount of RM per chamber (µl) x number of cells in RM amount of RM (µl) At a dose of 1 µg/mouse, the compound 1 caused a significant increase (p < 0.05) of the number of plaques from 86 ± 12.7 (n = 8) to 170 ± 39.9 (n = 8) and to 245 ± 75.5 (n = 7) respectively. The values were reported as x ± s.e.m (n = number of samples). 2. Antitumour activity The studies were performed on A/J strain mice (Institute Rudjer Bo kovi , Zagreb, Yugoslavia) of 8 to 10 weeks. All animals used in the same experiment were of the same sex and age. Fibrosarcoma SA-1 was used as the experimental tumour model. Tumorous cells were obtained from the ascitic form of the SA-1 tumour of the syngeneic A/J mouse. They were implanted into animals dorsilaterally by s.c. injection of 5 x 10 5 live tumour cells. The testing was started after the tumours had grown to a volume of 35 mm 3 . The test substance was injected i.p. on five consecutive days in a dose of 2.5 or 25 µ g. The growth of the tumours was monitored by measuring the diameters and the thickness of the tumours. The tumour volumes were calculated according to the formula 0.523 x a x b x c (a, b and c being the tumour diameters). The delay in the growth of each individual tumour was obtained by deducting the relevant time of growth of the tumour in the control group from the time needed for the growth of the tumour to reach the volume of 150 mm 3 . In comparison to the control, the antitumour activity of the compound 1 was moderate and statistically significant at the dose of 2.5 µg (p < 0.002) and at the dose of 25 µ g (p < 0.05). However, there was no statistically significant difference in activity between the higher and the lower dose. Time needed for the tumour growth to the volume of 150 mm 3 and the delay in tumour growth Substance n Number of days (± SD) Growth delay (± SD) p (compared with control) Control 22 5.5 ± 0.8 Compound 1 2.5 µg 10 7.3 ± 1.5 1.8 ± 0.5 < 0.01 25.0 µg 11 7.0 ± 1.7 1.5 ± 0.5 < 0.05 n = number of experiments 3. Pyrogenous activity The pyrogenous activity was determined according to the method of USP XXII. In contrast to MDP, compound 1 did not show any pyrogenous activity. 4. Toxicity The average lethal dose (LD 50 ) at i.v. application of the compound 1 in male mice was > 250 mg/kg. All above-described immunological effects of the compound 1 appeared already at a dose which was at least 2000 times lower than LD 50 . Evaluation of compound 1 Compound 1 is an immunomodulator, which enhances the maturation of lymphocytes B to plasma cells and increases the activity of lymphocytes T as well as that of macrophages but does not alter the number of these immunologically competent cells. In the tested range the effect is dose-dependent. As an immunomodulator it also shows an antitumour activity. In contrast to muramyl dipeptide, which similarly acts upon different immunologically competent cells, compound 1 is neither pyrogenous nor very toxic. Pharmaceutical preparations The pharmaceutical preparations of the invention can be in the form of coated pills, tablets, capsules, ampoules or aerosols to be used on mucous membranes. Preparations suitable for parenteral application can also comprise liposomes. The pharmaceutical preparations of the invention comprise the pharmacologically active compound alone or together with a pharmaceutically acceptable carrier chosen with respect to the mode of application. Pharmaceutical preparations are manufactured according to methods known per se . The dose, the frequency and the mode of application will depend on various factors such as the intended use (e.g. for the treatment of the primary or secondary immunodeficiency or of various types of infections or for increasing the antitumour activity). A suitable dose for an adult wil be from 0.1 to 250 mg/day. The exact dose, the frequency and the mode of application will be chosen with respect to the activity and pharmacokinetic properties of the particular compound and to other factors that can affect the effect of the drug, such as the type and severity of the condition, the patient's age, weight, sex and response to the medication. Injections containing 1 ml each Compound 1 0.1 mg Sodium chloride 9.0mg Sodium hydroxyde q.s. Water for injections 1 ml The invention is illustrated by the following Examples. Starting compounds EXAMPLE 1 N-(2-(2-hydroxyethoxy)-ethyl)-4-chloro-benzamide To a solution of sodium hydroxyde (2.2 g, 0.055 moles) in distilled water (100 ml), aminoethoxyethanol (5.25 g, 0.05 moles) was added and the solution was cooled to 0 °C on an ice bath. Under stirring on the ice bath, p-chloro-benzoylchloride (8.75 g, 0.5 moles) was added thereto drop by drop. The stirring was continued for 3 hours at room temperature and the separated precipitate was filtered off by suction. The mother liquid was extracted with chloroform (3 x 100 ml). The organic phase was separated, dried with sodium sulfate, filtered and evaporated on a rotary evaporator. The precipitated N-O-di-((4-chloro)-benzoyl)-amino-ethoxyethanol was dissolved in dioxan (50 ml) and sodium hydroxyde (22 ml of a 1 N solution) was added thereto. The solution was stirred for 2 hours at 40 °C, water (50 ml) was added thereto and it was extracted with ethyl acetate (5 x 50 ml). The organic phase was separated, dried with sodium sulfate, filtered and evaporated on a rotary evaporator. The obtained product was crystallized from diethyl ether. Yield: 9.4 g (77.1%) Melting point: 83-84 °C 1 H-NMR (CDCl 3 ): δ = 2.57 (s, 1H, - OH ), 3.48-3,90 (m, 8H, -NH CH 2 CH 2 OCH 2 CH 2 OH), 6.93 (s (broad), 1H, - NH -), 7.38 (d, 2H, aromatic, H 2 , H 6 ), 7.79 (d, 2H, aromatic, H 3 , H 5 .). J = 9 Hz IR (KBr, cm -1 ): 3350, 2980, 2900, 1645, 1600, 1550, 1125, 900, 835, 760 Analysis for C 11 H 14 ClNO 3 : %C %H %N calc. 54.22 5.79 5.75 found 54.33 5.98 5.59 EXAMPLE 2 N-(2-(2-hydroxyethoxy)-ethyl)-benzamide To a solution of sodium hydroxyde (2.2 g, 0.055 moles) in distilled water (100 ml), aminoethoxyethanol (5.25 g, 0.05 moles) was added and then, under stirring, benzoyl chloride (7.03 g,0.05 moles) was added drop by drop at such a rate that the temperature did not exceed 35 °C. The stirring was continued for 2 hours at room temperature and the reaction mixture was extracted with chloroform (5 x 100 ml). The organic phase was separated, dried with sodium sulfate, filtered and evaporated on a rotary evaporator. The product was purified by column chromatography (mobile phase chloroform/methanol 9:1). The product was a viscous liquid. Yield: 7.8 g (74.6%) 1 H-NMR (CDCl 3 ): δ = 3.58-3.64 (m, 8H, NH CH 2 CH 2 OCH 2 CH 2 OH), 7.33-7.84 (m, 5H, phenyl). IR (NaCl film, cm -1 ): 3340, 2950, 2890, 1650, 1550, 1500, 1320, 1130, 1080, 720 EXAMPLE 3 N-(2-(2-hydroxyethoxy)-ethyl)-phthalimide Carbethoxyphthalimide (5.20 g, 0.024 moles) was dissolved in anhydrous tetrahydrofuran (30 ml), the solution was cooled on an ice bath to 0-5 °C and aminoethoxyethanol (2.37 g, 0.023 moles) in tetrahydrofuran (20 ml) was added drop by drop under stirring. The stirring was continued for 15 minutes on the ice bath and subsequently for 24 hours at room temperature. The solvent was evaporated on a rotary evaporator and the compound was purified by gel chromatography on Sephadex (mobile phase chloroform/methanol 1:1) and crystallization from ethyl acetate. Yield: 4.32 g (79.8%) Melting point: 63-64 °C 1 H-NMR (CDCl 3 ): δ = 3.40-4.00 (m, 8H, -N CH 2 CH 2 OCH 2 CH 2 OH), 7.40-7.80 (m, 4H, phthaloyl). Molecular weight: calculated: 235.24 found: 235 IR (KBr, cm -1 ): 3560, 2900, 1720, 1480, 1440, 1405, 1370, 1200, 1120, 1090, 1030, 930, 890, 805, 730 Elemental analysis for C 12 H 13 NO 4 : %C %H %N calc. 61.27 5.57 5.95 found 61.39 5.76 6.01 EXAMPLE 4 2-(2-benzamidoethoxy)-acetic acid N-(2-(2-hydroxyethoxy)-ethyl)-benzamide (3.14 g, 0.015 moles) was dissolved in acetone (80 ml) and the solution was cooled to 0 °C. A solution of chromium(VI) oxide (3.75 g, 0.0375 moles) in sulfuric acid (35%, 78.5 ml) was added drop by drop under stirring at the temperature of 0-5 °C. The stirring was continued at room temperature for 2.5 hours, whereafter the reaction mixture was poured into water (350 ml) and extracted with ethyl acetate (4 x 100 ml). The organic phase was separated, dried with sodium sulphate, filtered and evaporated on a rotary evaporator. The product was purified by crystallisation from acetone. Yield: 2.5 g (74.7%) Melting point: 128-130°C 1 H-NMR (DMSO-d 6 ): δ = 3.18 (s, 2H, -O CH 2 COOH), 3.70-3.79 (m, 4H, - NHCH 2 CH 2 O-), 7.44-7.99 (m, 6H, phenyl, - NH -), 8.01 (s, 1H, - COOH ). IR (KBr, cm -1 ): 3320, 2885, 1720, 1625, 1550, 1220, 1150, 875, 715 Elemental analysis for C 11 H 13 NO 4 : %C %H %N calc. 59.14 5.87 6.27 found 59.48 6.24 6.28 EXAMPLE 5 2-(2-(4-chlorobenzamide)-ethoxy)-acetic acid The title compound was prepared from N-(2-(2-hydroxyethoxy)-ethyl)-4-chlorobenzamide in a manner analogous to the one described in Example 4. Yield: 1.5 g (71.0%) Melting point: 127-130°C 1 H-NMR (DMSO-d 6 ): δ = 3,13-3,73 (m, 4H, -NH CH 2 CH 2 O-), 4,06 (s, 2H, -O CH 2 COOH), 7,55 (d, 2H, aromatic, H 2 , H 6 .), 7,90 (d, 2H, aromatic, H 3 , H 5 ,). J = 9 Hz IR (KBr, cm -1 ): 3350, 2910, 1730, 1630, 1570, 1200, 1145, 1015, 850, 755 Elemental analysis for C 11 H 12 ClNO 4 : %C %H %N calc. 51.27 4.69 5.44 found 51.37 4.78 5.38 EXAMPLE 6 2-(2-phthalimidoethoxy)-acetic acid The title compound was prepared from N-(2-(2-hydroxyethoxy)-ethyl)-phthalimide in a manner analogous to the one described in Example 4. Yield: 1.0 g (80.2%) Melting point: 127-129 °C 1 H-NMR (DMSO-d 6 ): δ = 3.69-3.77 (m, 4H, -N CH 2 CH 2 O-), 3.99 (s, 2H, -O CH 2 COOH), 7.85 (s, 4H, phthaloyl). Molecular weight: calculated: 249.23 found: 249 IR (KBr, cm -1 ): 3220, 1765, 1695, 1400, 1190, 1130, 1045, 1000, 730 Elemental analysis for C 12 H 11 NO 5 : %C %H %N calc. 57.83 4.45 5.62 found 57.80 4.51 5.66 EXAMPLE 7 N-(2-(2-hydroxyethoxy)ethyl)-1-adamantanecarboxamide The title compound was prepared from aminoethoxyethanol in a manner analogous to the one described in Example 1. Yield: 82% Melting point: 72-73 °C 1 H-NMR(CDCl 3 ): δ = 1.71-2.1 (3m, 15H, adamantyl), 2.58 (broad s, 1H, OH), 3.42-3.50 (m, 2H, -NH CH 2 -), 3.54-3.62 (m, 4H, 2CH 2 ), 3.74 (t, 2H, CH 2 , J=4.6 Hz), 6.15 (s, 1H, NH). IR(KBr, cm -1 ): 3346, 2903, 1633, 1531, 1452, 1285, 1133, 1070, 889. Elemental analysis for C 15 H 25 NO 3 % C % H % N calc. 67.38 9.42 5.24 found 67.17 9.55 5.07 EXAMPLE 8 N-(2-(2-hydroxyethoxy)-ethyl)-p-toluenesulphonamide The title compound was prepared from aminoethoxyethanol in a manner analogous to the one described in Example 1. Yield: 50 % Melting point: 35-38 °C 1 H-NMR(CDCl 3 ): δ = 2.42 (S, 3H, CH 3 ), 3.12 (t, 2H, J=5.3 Hz, -NH- CH 2 -), 3.48-3.51 (m, 4H, 2CH 2 ), 3.66-3.69 (m, 2H, CH 2 ), 5.97 (s, 1H, NH), 7.2-7.4 (m, 5H, phenyl). IR(KBr, cm -1 ): 3400, 2910, 2800, 1325, 1160, 1100, 1070. EXAMPLE 9 2-(2-(1-adamantanecarboxamido)-ethoxy)-acetic acid The title compound was prepared from N-(2-(2-hydroxyethoxy)ethyl)-1-adamantanecarboxamide in a manner analogous to the one described in Example 4. Yield: 86% Melting point: 120-124 °C 1 H-NMR(CDCl 3 ): δ = 1.62-2.14 (m, 15H, adamantyl), 3.4-3.53 (m, 2H, -NH- CH 2 -), 3.65 (t, 2H, J=4.7 Hz, -CH 2 CH 2 -O), 4.14 (s, 2H, CH 2 ), 6.36 and 6.71 (2t, 1H, -CO- NH -CH 2 -), 7,93 (broad s, 1H, -COOH). IR(KBr, cm -1 ): 3374, 2906, 2535, 1728, 1607, 1545, 1453, 1219, 1142, 969, 881, 667. Elemental analysis for C 15 H 21 NO 4 % C % H % N calc. 64.50 7.58 5.01 found 64.12 8.01 5.40 EXAMPLE 10 2-(2-(p-toluenesulphonamido)-ethoxy-acetic acid The title compound was prepared as an oil from N-(2-(2-hydroxyethoxy)-ethyl)-p-toluene- sulphonamide in a manner analogous to the one described in Example 4. Yield: 63% 1 H-NMR (CDCl 3 ): δ = 2.37 (s, 3H, CH 3 ), 2.88 (td, 2H, -NH- CH 2 -), 3.42 (t, 2H, J=6 Hz, - CH 2 -O-), 3.92 (s, 2H, - CH 2 COOH), 7.30-7.41 and 7.5-7.7 (m, 4H, phenyl), 12.57 (s, 1H, COOH). IR(KBr, cm -1 ): 3360, 3260, 2910, 2880, 1730, 1320, 1160, 1100. Compounds according to the invention EXAMPLE 11 N-(7-oxododecanoyl)-L-alanine methyl ester 7-oxododecanoic acid (1000 mg, 4.67 mmoles) and L-alanine methyl ester hydrochloride (656 mg, 4.7 mmoles) were dissolved in N,N-dimethylformamide (5 ml). The solution was cooled to 0 °C and diphenylphosphoryl azide (1300 mg, 4.72 mmoles) and triethylamine (950 mg, 0.95 mmoles) were added thereto. The reaction mixture was stirred for 1 hour on an ice bath and for 24 hours at room temperature. The resulting suspension was diluted with ethyl acetate (30 ml) and washed successively with citric acid (0.52 M; 3 x 5 ml), water (3 x 5 ml), saturated NaCI solution (3 x 5 ml), saturated NaHCO 3 solution (3 x 5 ml), water (3 x 5 ml) and saturated NaCI solution (3 x 5 ml). The organic phase was dried with MgSO 4 and filtered, the filtrate was evaporated and the compound was precipitated from n-hexane/diethyl ether. Yield: 64% Melting point: 66-70 °C Melting point determined by the DSC method: T max = 69.9 °C Molecular weight: calculated: 299.41 found: 299 Elemental analysis for C 16 H 29 NO 4 .1H 2 O %C %H %N calc. 60.54 9.84 4.41 found 60.15 9.42 4.36 IR (KBr, cm -1 ): 3340, 2980-2960, 2860, 1760, 1715, 1670-1650, 1350, 1460, 1390, 1290, 1180 EXAMPLE 12 N-(7-oxotetradecanoyl)-L-alanine methyl ester The title compound was prepared from 7-oxotetradecanoic acid in a manner analogous to the one described in Example 11. Yield: 83% Melting point: 61-64 °C Molecular weight: calculated: 327.47 found: 327 Elemental analysis for C 18 H 33 O 4 %C %H %N calc. 66.02 10.15 4.27 found 65.88 10.36 4.21 IR (KBr, cm -1 ): 3320, 2920-2910, 2850, 1730, 1690, 1630, 1530, 1440, 1370, 1220, 1170 EXAMPLE 13 N-(7-oxodecanoyl)-L-alanine methyl ester The title compound was prepared from 7-oxodecanoic acid in a manner analogous to the one described in Example 11. Yield: 63% Melting point: 51-54 °C Molecular weight: calculated: 271.36 found: 271 Elemental analysis for C 14 H 25 NO 4 %C %H %N calc. 61.99 9.22 5.16 found 61.68 9.63 5.46 IR (KBr, cm -1 ): 3300, 2920-2900, 2840, 1730, 1690, 1630-1320, 1530, 1440, 1370, 1220, 1150 EXAMPLE 14 N-(7-oxododecanoyl)-L-alanine N-(7-oxododecanoyl)-L-alanine methyl ester (700 mg, 2.34 mmoles) was dissolved in dioxane (10 ml) and a solution of NaOH (1 M; 4 ml) was slowly added thereto under stirring. After 20 minutes the reaction mixture was evaporated, the residue was diluted with water (25 ml) and cooled on an ice bath to 0 °C. The cooled solution was acidified with 1 M HCl to pH 2. The precipitate that separated was filtered off by suction (thus there were obtained 305 g of the product) and the filtrate was extracted with ethyl acetate (3 x 10 ml). A total of 630 mg of the product was obtained, with was crystallized from diethyl ether/methanol. Yield: 94% Melting point: 79-80 °C Molecular weight: calculated: 285.11 found: 285 Elemental analysis for C 15 H 27 NO 4 %C %H %N calc. 63.13 9.53 4.90 found 63.33 9.84 4.90 IR (KBr, cm -1 ): 3380-3360, 2980-2940, 2880, 1730-1700, 1650, 1550-1520, 1420, 1390, 1250 EXAMPLE 15 N-(7-oxotetradecanoyl)-L-alanine The title compound was prepared from N-(7-oxotetradecanoyl)-L-alanine methyl ester in a manner analogous to the one described in Example 14. Yield: 92% Melting point: 84-86 °C Molecular weight: calculated: 313.44 found: 313 Elemental analysis for C 17 H 31 NO 4 %C %H %N calc. 65.14 9.97 4.47 found 65.39 10.09 4.22 IR (KBr, cm -1 ): 3380-3360, 2980-2940, 2880, 1730-1700, 1670-1660, 1540-1520, 1470, 1420, 1390. EXAMPLE 16 N-(7-oxododecanoyl)-L-alanyl-D-isoglutamine benzyl ester N-(7-oxododecanoyl)-L-alanine (590 mg, 2.07 mmoles) and D-isoglutamine benzyl ester hydrochloride (565 mg, 2.07 mmoles) were dissolved in N,N-dimethylformamide (7 ml). The solution was cooled to 0 °C and diphenylphosphoryl azide (578 mg, 2.1 mmoles) and triethylamine (424 mg, 4.2 mmoles) were added thereto. The reaction mixture was stirred for 1 hour on an ice bath and for another 48 hours at room temperature. To the resulting suspension there were added benzene (5 ml) and ethyl acetate (25 ml) and it was washed successively with citric acid (0.52 M; 3 x 5 ml), water (3 x 5 ml), saturated NaCI solution (3 x 5 ml), saturated NaHCO 3 solution (3 x 5 ml), water (3 x 5 ml) and saturated NaCl solution (3 x 5 ml). The organic phase was dried with MgSO 4 and filtered, the filtrate was evaporated and the compound was precipitated from n-hexane/diethyl ether. Yield: 86% Melting point: 159-161 °C Melting point determined by the DSC-method: 161 °C Molecular weight: calculated: 503.64 found: 446(M-C 4 H 9 ) Elemental analysis for C 27 H 41 N 3 O 6 %C %H %N calc. 64.39 8.20 8.34 found 64.28 8.42 8.30 IR (KBr, cm -1 ): 3460-3440, 3300, 3220, 3080, 2980-2940, 2880, 1740, 1680, 1650-1610, 1560-1540, 1450, 1380, 1260, 1190. EXAMPLE 17 N-(7-oxotetradecanoyl)-L-alanyl-D-isoglutamine benzyl ester The title compound was prepared from N-(7-oxotetradecanoyl)-L-alanine in a manner analogous to the one described in Example 16. Yield: 88% Melting point: 137-141 °C Molecular weight: calculated: 531.69 found: 531 Elemental analysis for C 29 H 45 N 3 O 6 %C %H %N calc. 65.51 8.53 7.90 found 65.10 8.05 7.30 IR (KBr, cm -1 ): 3420, 3320-3280, 3200, 3080, 2960-2940, 2860, 1720, 1680, 1650-1620, 1560-1530, 1450, 1390, 1290, 1190. EXAMPLE 18 N-(7-oxooctanoyl)-L-alanyl-D-isoglutamine benzyl ester The title compound was prepared from N-(7-oxooctanoyl)-L-alanine in a manner analogous to the one described in Example 16. Yield: 55% Melting point: 162 °C Melting point determined by the DSC-method: T max = 162.2°C Molecular weight: calculated: 447.53 found: 339 (M-Bz) Elemental analysis for C 23 H 33 N 3 O 6 %C %H %N calc. 61.73 7.43 9.39 found 62.06 7.48 9.00 IR (KBr, cm -1 ): 3420, 3320, 3280, 3080, 2940, 2880, 1750, 1715, 1680, 1660, 1640-1610, 1550, 1460, 1430, 1390, 1270. EXAMPLE 19 N-(7-oxooctanoyl)-L-alanyl-D-isoglutamine (compound No. 1) N-(7-oxooctanoyl)-L-alanyl-D-isoglutamine benzyl ester (310 mg, 0.69 mmoles) was dissolved in methanol (20 ml) and Pd/C (10%; 130 mg) was added thereto. Hydrogen was passed in for 30 minutes, it was filtered and the filtrate was evaporated. The compound was crystallized from diethyl ether/ethanol. Yield: 81 % Melting point: 169-171°C Melting point determined by the DSC-method: T max = 172.4°C Molecular weight: calculated: 357.4 found: 357 Elemental analysis for C 16 H 27 N 3 O 6 %C %H %N calc. 53.77 7.61 11.75 found 53.42 7.75 11.42 IR (KBr, cm -1 ): 3420, 3320, 3280, 2960, 2880, 1680, 1660, 1650-1630, 1430. 1 H-NMR (300 MHz, DMSO-d 6 ): δ = 1.14-1.26 (m, 2H, CH 2 CH 2 CH 2 ), 1.15-1.21 (d, 3H, J=7.0 Hz, CH CH 3 ), 1.38-1.56 (m, 4H, J=7.38 Hz, CH 2 CH 2 CH 2 CH 2 CH 2 ), 1.62-1.76 and 1.92-2.02 (m, 1H each, CH 2 -β-iGln), 2.06 (s, 3H, CH 3 CO), 2.04-2.12 (t, 2H, J=7.33 Hz, CH 3 CO CH 2 CH 2 ), 2.14-2.24, (t, 2H, J=7.63 Hz, CH 2 COOH), 2.36-2.44 (t, 2H, J=7.33 Hz, CH 2 CONH), 4.09-4.23 (m, 2H, CH-iGln, CH-Ala), 7.09-7.2 and 7.45-7.75 (2s, 1H each, CONH 2 ), 8.05-8.15 (2d, 1H each, NH). EXAMPLE 20 N-(7-oxododecanoyl)-L-alanyl-D-isoglutamine N-(7-oxododecanoyl)-L-alanyl-D-isoglutamine benzyl ester (780 mg, 1.55 mmoles) was suspended in absolute methanol (20 ml) and Pd/C (10 %; 100 mg) was added thereto and hydrogen was passed in at normal pressure for 1 hour. The reaction mixture was filtered by suction, the catalyst was removed and the filtrate was evaporated. The solid product was precipitated from methanol/anhydrous diethyl ether. Yield: 76.5% Melting point: 148-151 °C Molecular weight: calculated: 413.14 found: 414 (M+1) Elemental analysis for C 20 H 35 N 3 O 6 %C %H %N calc. 58.09 8.53 10.16 found 57.87 8.23 9.46 IR (KBr, cm -1 ): 3420, 3300, 3220, 2980-2940, 2880, 1740-1700, 1680, 1650-1630, 1610, 1560-1530, 1460, 1430, 1390, 1250. EXAMPLE 21 N-(5-phthalimidopentanoyl)-L-alanyl-D-isoglutamine benzyl ester L-alanyl-D-isoglutamine benzyl ester hydrochloride (548 mg, 1.6 mmoles) and 5-phthalimidopentanoic acid (400 mg, 1.6 mmoles) were dissolved in N,N-dimethylformamide (8 ml). The solution was cooled to 0 °C and diphenylphosphoryl azide (495 mg, 1.8 mmoles) and triethylamine (323 mg, 3.2 mmoles) were added thereto. The reaction mixture was stirred for 1 hour at 0 °C and for 24 hours at room temperature. The reaction mixture was diluted by ethyl acetate (40 ml) and the organic phase was washed successively with citric acid (0.52 M; 3 x 10 ml), water (3 x 10 ml), saturated NaCl solution (3 x 10 ml), saturated NaHCO 3 solution (3 x 10 ml), water (3 x 10 ml) and saturated NaCl solution (3 x 10 ml). The organic phase was dried with MgSO 4 and filtered, the filtrate was evaporated and the product was precipitated from n-hexane/diethyl ether. The compound was filtered off and crystallized from acetone. Yield: 65% Melting point: 172-174°C Melting point determined by the DSC-method: T max = 174.7°C Molecular weight: calculated: 536.59; found: 446 (M-Bz) Elemental analysis for C 28 H 32 N 4 O 7 %C %H %N calc. 62.67 6.01 10.44 found 62.74 6.28 10.48 IR (KBr, cm -1 ): 3420, 3300, 3080, 2980-2940, 2880, 1750, 1730-1700, 1680, 1650-1630, 1410, 1190. EXAMPLE 22 N-(5-phthalimidopentanoyl)-L-alanyl-D-glutamic acid dibenzyl ester L-alanyl-D-glutamic acid dibenzyl ester hydrochloride (685 mg, 2 mmoles) and 5-phthalimidopentanoic acid (494 mg, 2 mmoles) were dissolved in N,N-dimethylformamide (8 ml). The solution was cooled to 0 °C and diphenylphosphoryl azide (604 mg, 2.2 mmoles) and triethylamine (404 mg, 4 mmoles) were added thereto. The reaction mixture was stirred for 1 hour at 0 °C and for 48 hours at room temperature. The reaction mixture was diluted by ethyl acetate (50 ml) and successively washed with citric acid (0.52 M; 3 x 10 ml), water (3 x 10 ml), saturated NaCl solution (3 x 10 ml), saturated NaHCO 3 solution (3 x 10 ml), water (3 x 10 ml) and saturated NaCl solution (3 x 10 ml). The organic phase was dried with MgSO 4 and filtered, the filtrate was evaporated and the product was precipitated from diethyl ether. The analytically pure product was obtained after filtration by suction. Yield: 70% Melting point: 114-117 °C Molecular weight: calculated: 627.7 found: 627 Elemental analysis for C 35 H 37 N 3 O 8 %C %H %N calc. 66.97 5.94 6.69 found 66.62 6.13 6.31 IR (KBr, cm -1 ): 3320, 3080, 2980-2940, 1740-1710, 1660-1640, 1560-1540, 1450, 1420, 1350, 1280-1260, 1180-1160. EXAMPLE 23 N-(4-phthalimidobutanoyl)-L-alanyl-D glutamic acid dibenzyl ester The title compound was prepared from 4-phthalimidobutanoic acid in a manner analogous to the one described in Example 22. Yield: 65% Melting point: 88-90 °C Molecular weight: calculated: 613.67 found: 613 Elemental analysis for C 34 H 35 N 3 O 8 %C %H %N calc. 66.54 5.75 6.84 found 66.83 6.04 6.44 IR (KBr, cm -1 ): 3320, 3080, 2980, 2960, 1750-1720, 1650-1630, 1460, 1390, 1280. EXAMPLE 24 N-(5-phthalimidopentanoyl)-L-alanyl-D-glutamic acid To N-(5-phthalimidopentanoyl)-L-alanyl-D-glutamic acid dibenzyl ester (310 mg, 0.49 mmoles) in methanol (30 ml), Pd/C (10%; 140 mg) was added and it was hydrogenated at atmospheric pressure for 3 hours. The reaction mixture was filtered, the filtrate was evaporated and the product was crystallized from diethyl ether/methanol. Yield: 75% Melting point: 101-105 °C Molecular weight: calculated: 447.45 found: 448 Elemental analysis for C 21 H 25 N 3 O 8 %C %H %N calc. 56.37 5.63 9.38 found 56.17 5.85 9.48 IR (KBr, cm -1 ): 3320-3280, 3080, 2960-2940, 1730-1710, 1650, 1560-1530, 1410, 1340 1 H-NMR (300 MHz, DMSO-d 6 ): δ = 1.2 (d, 3H, CH CH 3 ), 1.3-1.65 (m, 1H each, CH 2 -β-Glu), 2.05-2.19 (t, 2H, CH 2 -γ-Glu), 2.2-2.39 (t, 2H, J=5.61 Hz, CH 2 CONH), 3.4-3.55 (t, 2H, J=5.43 Hz, CH 2 NH), 4.1-4.2 (m, 1H, CH-Glu), 4.2-4.35 (m, 1H, CH-Ala), 7.7-7.9 (s, 4H, phthaloyl), 8.12 (s, 2H, NH). EXAMPLE 25 N-(2-(2-(4-chlorobenzamido)-ethoxy)-acetyl)-L-alanyl-D-isoglutamine benzyl ester The title compound was prepared from 2-(2-(4-chlorobenzamido)-ethoxy)-acetic acid in a manner analogous to the one described in Example 21. Yield: 67% Melting point: 141-143 °C 1 H-NMR(DMSO-d 6 ): δ = 1.21 (d, 3H, J=7.0 Hz, CH CH 3 ), 1.69-1.86 and 1.93-2.10 (2m, 1H each, CH 2 -β-Gln), 2.36 (t, 2H, J=7.8 Hz, CH 2 -γ-Gln), 3.46 (t, 2H, J=5.1 Hz, N CH 2 CH 2 O), 3.61 (t, 2H, NCH 2 CH 2 O), 3.93 (s, 2H, -O CH 2 CO), 4.11-4.25 (m, 1H, CH-Gln), 4.33 (t, 1H, J=7.03 Hz, CH-Ala), 5.08 (s, 2H, CH 2 Ph), 7.14 and 7.32 (2s, 1H each, CONH 2 ), 7.35 (s, 5H, phenyl), 7.53 (d, 2H, J=8.6 Hz, H 2 , in H 6 .), 7.85 (d, 1H, J=8.1 Hz, NH), 7.88 (d, 2H, J=8.6 Hz, H 3 . in H 5 .), 8.21 (d, 1H, J=8.1 Hz, NH), 8.70 (t, 1H, J=5.4 Hz, CO NH CH 2 ). IR(KBr, cm -1 ): 3380, 3290, 2940, 1725, 1660, 1540, 1325, 1175, 1090, 1015, 760, 700. Elemental analysis for C 26 H 31 N 4 O 7 % C % H % N calc. 57.09 5.71 10.24 found 57.07 5.99 10.15 EXAMPLE 26 N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-isoglutamine benzyl ester The title compound was prepared from 2-(2-phthalimidoethoxy)-acetic acid in a manner analogous to the one described in Example 21. Yield: 88% Melting point: 205-206 °C 1 H-NMR(DMSO-d 6 ): δ = 1.19 (d, 3H, J=6.8 Hz, CH CH 3 ), 1.68-1.85 and 1.93-2.10 (2m, 1H each, CH 2 -β-Gln), 2.35 (t, 2H, J=7.7 Hz, CH 2 -γ-Gln), 3.68 (t, 2H, J=5.0 Hz, N CH 2 CH 2 O), 3.82 (t, 2H, NCH 2 CH 2 O), 3.91 (s, 2H, -O CH 2 CO), 4.10-4.21 (m, 1H, CH-Gln), 4.27 (t, 1H, J=6.10 Hz, CH-Ala), 5.08 (s, 2H, COO CH 2 Ph), 7.11 and 7.33 (2s, 1H each, CONH 2 ), 7.36 (s, 5H, phenyl), 7.81-7.86 (m, 4H, phthaloyl), 7.69 and 8.16 (2d, 1H each, NH, J=7.6 Hz). IR(KBr, cm -1 ): 3340, 3380, 3275, 3050, 2945, 1765, 1710, 1660, 1515, 1390, 1270, 1195, 1140, 1030, 1020, 760, 725. Elemental analysis for C 27 H 30 N 4 O 8 % C % H % N calc. 60.22 5.61 10.40 found 60.09 5.78 10.40 EXAMPLE 27 N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-glutamic acid dibenzyl ester The title compound was prepared from 2-(2-phthalimidoethoxy)-acetic acid in a manner analogous to the one described in Example 22. Yield: 66% Melting point: 108-109 °C 1 H-NMR(CDCl 3 ): δ = 1.40 (d, 3H, J=7.1 Hz, CH CH 3 ), 1.68-1.85 and 1.92-2.07 (2m, 1H each, CH 2 -β-Glu), 2.39 (t, 2H, J=8.1 Hz, CH 2 -γ-Glu), 3.64-3.77 (m, 2H, J=5.1 Hz, N CH 2 CH 2 O), 3.86-3.97 (m, 4H, NCH 2 CH 2 O and O CH 2 CO), 4.4-4.52 (m, 1H, CH -Glu), 4.53-4.63 (m, 1H, CH-Ala), 5.08 and 5.1 (2s, 2H each, COO CH 2 Ph), 7.07 and 7.11 (2s, 1H each, NH), 7.27-7.40 (m, 10H, phenyl), 7.66-7.89 (m, 4H, phthaloyl). IR(KBr, cm -1 ): 3302, 1774, 1745, 1719, 1699, 1649, 1521, 1406, 1270, 1207, 1187, 1115, 1022, 870. Elemental analysis for C 34 H 35 N 3 O 9 % C % H % N calc. 64.86 5.60 6.67 found 64.72 5.71 6.76 EXAMPLE 28 N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-isoglutamine N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-isoglutamine benzyl ester (0.1 g, 1.9 mmoles) was dissolved in glacial acetic acid (10 ml), Pd/C (10%; 0.015 g) was added thereto and hydrogen was passed in for 6 hours. The reaction mixture was filtered and the filtrate was evaporated. Yield: 85% Melting point: 229-232 °C 1 H-NMR(DMSO-d 6 ): δ = 1.20 (d, 3H, J=6.8 Hz, CH CH 3 ), 1.79-1.89 and 1.94-2.02 (2m, 1H each, CH 2 -β-Gln), 2.19 (t, 2H, J=7.8 Hz, CH 2 -γ-Gln), 3.69 (t, 2H, J=5.3 Hz, N CH 2 CH 2 O), 3.81 (t, 2H, NCH 2 CH 2 O), 3.91 (s, -O CH 2 CO), 4.03-4.09 (m, 1H, CH-Gln), 4.28 (t, 1H, J=7.02 Hz, CH-Ala), 7.09 and 7,29 (2s, 1H each, CO NH 2 ), 7.68 (d, 1H J=7.0 Hz, NH), 7.89-7.99 (m, 4H, phthaloyl), 8.14 (d, 1H, NH, J=81 Hz, NH). IR(KBr, cm -1 ): 3440, 3360, 3274, 2910, 1755, 1665, 1635, 1540, 1390, 1130. Elemental analysis for C 20 H 24 N 4 O 8 % C % H % N calc. 53.57 5.39 12.49 found 53.36 5.65 12.12 EXAMPLE 29 N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-glutamic acid The title compound was prepared from N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D glutamic acid dibenzyl ester in a manner analogous to the one described in Example 28. Yield: 55% Melting point: 192-195 °C 1 H-NMR(DMSO-d 6 ): δ = 1.20 (d, 3H, J=6.9 Hz, CH CH 3 ), 1.68-1.84 and 1.90-2.06 (2m, 1H each, CH 2 -β-Glu), 2.41 (t, 2H, J=7.3 Hz, CH 2 -γ-Glu), 3.69 (t, 2H, J=5.2 Hz, N CH 2 CH 2 O), 3.81 (t, 2H, NCH 2 CH 2 O), 3.91 (s, 2H, -O CH 2 CO), 4.14-4.24 (m, 1H, CH-Gln), 4.28-4.41 (m, 1H, CH-Ala), 7.57 (d, 1H, NH), 7.80-7.93 (m, 4H, phthaloyl), 8.24 (d, 1H, NH), 12.4 (broad s, 2H, COOH). IR(KBr, cm -1 ): 3372, 3311, 2952, 1768, 1747, 1708, 1660, 1636, 1559, 1393, 1205, 1135, 1021, 719. Elemental analysis for C 20 H 23 N 3 O 9 % C % H % N calc. 53.45 5.16 9.35 found 53.40 5.22 9.39 EXAMPLE 30 N-(2-(2-(1-adamantanecarboxamido)-ethoxy)-acetyl)-L-alanyl-D-isoglutamine benzyl ester The title compound was prepared from 2-(2-(1-adamantanecarboxamido)-ethoxy)-acetic acid in a manner analogous to the one described in Example 21. The compound was purified by column chromatography on silica gel with acetone as the mobile phase. Yield: 35% Melting point: 57-64 °C 1 H-NMR(CDCl 3 ): δ = 139 (d, 3H, J=7.0 Hz, CH CH 3 ), 1.65-2.02 (m, 15H, adamantyl), 1.93-2.02 and 2.17-2.26 (2m, 1H each, CH 2 -β-Gln), 2.48 (t, 2H, J=7.3 Hz, CH 2 -γ-Gln), 3.45 (t, 2H, J=5.1 Hz, N CH 2 CH 2 O), 3.57 (t, 2H, NCH 2 CH 2 O), 3.96 (s, 2H, -O CH 2 COOH), 4.39-4.50 (m, 1H, CH-Gln), 4.30-4.58 (m, 1H, CH-Ala), 5.11 (s, 2H, COO CH 2 Ph), 6.23 and 6.32 (2s, 1H each, CONH 2 ), 6.98 (broad s, 1H, NH), 7.25-7.34 (m, 5H, phenyl), 7.73 (d, 1H, J=8.1 Hz, NH). IR(KBr, cm -1 ): 3405, 2906, 1734, 1654, 1534, 1452, 1385, 1261, 1167, 1116, 978, 910, 752, 698. EXAMPLE 31 N-(2-(2-(1-adamantanecarboxamido)-ethoxy)-acetyl)-L-alanyl-D-isoglutamine The title compound was prepared from N-(2-(2-(1-adamantanecarboxamido)-ethoxy)-acetyl)-L-alanyl-D-isoglutamine benzyl ester in a manner analogous to the one described in Example 20. Yield: 97% Melting point: 84-86 °C 1 H-NMR(DMSO-d 6 ): δ = 1.25 (d, 3H, J=6.9 Hz, CH CH 3 ), 1.58-2.06 (m, 17H, adamantyl and CH 2 -β-Gln); 2.20 (t, 2H, J=7.6 Hz, CH 2 -γ-Gln), 3.24-3.45 (t, 2H, J=5.2 Hz, N CH 2 CH 2 O), 3.46 (t, 2H, NCH 2 CH 2 O), 3.88 (s, 2H, -O CH 2 COOH), 4.10-4.26 (m, 1H, CH-Gln), 4.28-4.40 (m, 1H, CH-Ala), 7.10 (broad s, 1H, NH ), 7.47 (t, 1H, J=5.4 Hz, NH), 7.78 (d, 1H, J=7.1 Hz, NH), 8.16 (d, 1H, J=8.4 Hz, NH). IR(KBr, cm -1 ): 3372, 2906, 1707, 1654, 1533, 1450, 1287, 1255, 1173, 1137, 977, 673, 583.

Claims: N-acyldipeptide which is N-(7-oxooctanoyl)-L-alanyl-D-isoglutamine, N-(7-oxododecanoyl)-L-alanyl-D-isoglutamine, N-(5-phthalimidopentanoyl)-L-alanyl-D-isoglutamine, N-(2-(2-phthalimidoethoxy)-acetyl)-L-alanyl-D-glutamic acid or (N-(2-(2-(1-adamantanecarboxamido)-ethoxy)-acetyl)-L-alanyl-D-isoglutamine. Pharmaceutical compositions containing as the active ingredient a therapeutically effective amount of N-acyldipeptide of claim 1 together with common pharmaceutically acceptable carriers and excipients.

Inventors: IHAN ALOJZ DR, KIKELJ DANIJEL DR, KLAMFER LIDIJA MAG, KOPITAR ZDRAVKO PROF DR, KOTNIK VLADO DR, KRBAVCIC ALES PROF DR, MARC GASPER, PECAR SLAVKO PROF DR, POVSIC LUCKA, SIMCIC SASA, SOLLNER MARIJA, STALC ANTON DR, URLEB UROS DR, WRABER-HERZOG BRANKA, IHAN, ALOJZ, DR., KIKELJ, DANIJEL, DR., KLAMFER, LIDIJA, MAG., KOPITAR, ZDRAVKO, PROF. DR., KOTNIK, VLADO, DR., KRBAVCIC, ALES, PROF. DR., MARC, GASPER, PECAR, SLAVKO, PROF. DR., POVSIC, LUCKA, SIMCIC, SASA, SOLLNER, MARIJA, STALC, ANTON, DR., URLEB, UROS, DR., WRABER-HERZOG, BRANKA
Applicants: LEK TOVARNA FARMACEVTSKIH, UNIV LJUBLJANI, LEK, TOVARNA FARMACEVTSKIH IN KEMICNIH, UNIVERZA V LJUBLJANI, FAKULTETA ZA FARMACIJO
Application year: 1991
Main classification: C07K5
IPC codes: A61P37, C07K1, C07K5, A61K38, A61P17