Use of uridine in the pharmacological treatment of the peripheral complications of diabetes

Abstract: Uridine is used to treat the peripheral complications of diabetes, such as neuropathy, retinopathy and vasculopathy, thanks to its characteristics of promotor of glycogen endocellular biosynthesis. 40 diabetic patients were treated for six months with uridine or with placebo in a double-blind clinical test.

Description: The present invention refers to a new therapeutic use of uridine in the field of peripheral complications in diabetes mellitus. Uridine is a known endogenous compound which has been studied in the past as a pharmacological agent in many experimental models, even those in no way related to each other. It has, in fact, been shown that cytidine and uridine are capable of prolonging the functional survival of an isolated cat brain. Other researchers have brought to light important anticonvulsive properties of uridine. More recently, uridine has been proposed as a substance promoting sleep, as a substitute for a renal natriuretic system, or as a dopaminergic modulator of the central nervous system. It is furthermore universally known from classical biochemistry texts that uridine is the most important carrier of glucose within the cell, and that glycogen can only be formed upon intervention of uridine. It has also been shown that cytidine and uridine are capable of consenting the normal use of glucose in cirrhotic patients treated with insulin. It has furthermore been shown that uridine increases the formation of glycogen in the muscles and that uridine can antagonize certain effects due to insulin hypoglycemia. The peripheral complications in diabetes mellitus include a number of disabling situations, such as neuropathy, retinopathy, vasculopathy, etc. due to the presence in the blood of high quantities of glucose, which can spread passively in all types of cell not provided with specific "carriers". If the endocellular glucose exceeds the energy requirement, and is not stored in the form of polysaccharides, it can damage the cell, both because it changes into fructose and sorbitol (sugars which do not easily spread outwards, and which for this reason cause the cell to swell and lose functional activity), and because it can react with proteins and nucleic acids, bringing about a form of premature "cell aging". To relieve the peripheral symptomatology of diabetes mellitus, certain drugs have recently been proposed in the field of therapy. These drugs, by inhibiting the enzyme "aldosoreductase", prevent the glucose from transforming itself into sorbitol, thus limiting the damage caused by cellular oedema (see for example Annual Reports in Medicinal Chemistry 19, 169-177, 1984). At least in short-term clinical tests, these compounds have shown themselves to be of use to antagonize diabetic neuropathies (see for example: Lancet II, 758-762, 1983; New England J. Medicine 316, 599-606, 1987). However, these synthetic derivatives are not without side-effects which could compromise their long-term use, as in theory the diabetic patient would have to be treated all his life. It is therefore necessary to find physiological compounds that, as well as being active, are also free from serious undesirable effects. It has now been surprisingly found, and forms the object of the present invention, that uridine possesses these properties and can be used to decrease the peripheral symptomatology of diabetes, without causing side-effects even in the case of long-term treatment. Uridine can thus be administered to patients suffering from diabetes mellitus for the pharmacological treatment of peripheral complications such as neuropathy, retinopathy or vasculopathy. It is thought that the uridine, which is able to enter with ease into the cells, can store the glucose present therein under the form of glycogen. In order to evaluate at an experimental level the intervention of uridine upon the peripheral symptoms of diabetes, the following experimental tests have been performed. Experimental pattern Fourty diabetic patients (25 male and 15 female) were selected, having an average age of 48,5 ± 3,4 years, with a medical history of at least 5 years of diabetes, showing a reduction of the speed of motorial conductivity (VCM), and of the speed of sensorial conductivity (VCS) in at least one peripheral nerve, persistant pain in the lower limbs, reduction of the threshold of vibration perception. After having undergone a "wash-out" period of two weeks, to suspend all pharmacological treatment that might interfere with the evaluation of the parameters to be examined, the patients were divided randomly into two groups: the first group received 300 mg of uridine three times per day; the second group received similar capsules containing placebo. Neither the patients nor the doctors knew who was being treated with placebo and who with uridine (double-blind test). Treatment continued for 180 consecutive days. Clinical and neurophysiological evaluation took place at the following times: prebasal, basal (after two weeks "wash-out"), at 60 days, 120 days and 180 days, and after 90 days from the end of treatment as a follow-up. All patients were evaluated after a general and neurological check-up, ECG (electrocardiogram), haematological, urine and glycosilate haemoglobin (HnAlc) tests. The statistical calculation was carried out using the Student test and with the two-way Anova test. Results None of the patients had to suspend treatment due to side-effects, and this gives an indication of the optimum tolleration of uridine, a fact which can also be seen from the absence of significant differences between the two groups as far as the haematological, ECG, urinary and glycosilate haemoglobin tests are concerned. The statistical test showed differences both in the VCM and in the VCS. These differences became significant at the 120th day and remained so both at the 180th and during the follow-up period. Average VCM ± SD (m/sec) of the SPE in diabetics treated with uridine and with placebo. URIDINE PLACEBO Student Anova Pre-basal 38.1+1.8 38.4+2.3 N.S. N.S. Basal 37.4+2.3 38.0+2.7 N.S. N.S. Day 60 37.7+2.2 38.1+2.4 N.S. N.S. Day 120 40.9+2.4 38.2+2.4 p<0.05 p<0.01 Day 180 43.5+1.9 38.6+2.4 p<0.01 p<0.001 Follow-up 43.0+1.4 38.4+2.5 p<0.05 p<0.001 SPE = outer sciatic popliteal nerve SD = standard deviation Average VCM ± SD (m/sec) of the SPI in diabetics treated with uridine and with placebo. URIDINE PLACEBO Student Anova Pre-basal 34.9+2.1 35.3+2.4 N.S. N.S. Basal 34.8+1.6 34.9+1.8 N.S. N.S. Day 60 35.7+1.8 35.5+1.9 N.S. N.S. Day 120 39.5+2.1 35.4+2.7 p<0.005 p<0.005 Day 180 42.4+1.6 35.8+1.7 p<0.0005 p<0.001 Follow-up 41.3+1.1 35.3+2.1 p<0.001 p<0.001 SPI = inner sciatic popliteal nerve Average amplitude ± SD (microV) of the motorial response of the SPI in diabetics treated with uridine and with placebo. URIDINE PLACEBO Student Anova Pre-basal 6.3+3.2 6.2+2.7 N.S. N.S. Basal 6.1+2.6 6.1+2.4 N.S. N.S. Day 60 6.4+2.6 6.3+2.5 N.S. N.S. Day 120 7.4+2.8 6.4+2.2 N.S. p<0.01 Day 180 8.7+3.0 6.2+2.4 p<0.05 p<0.01 Follow-up 8.5+3.1 6.1+2.2 p<0.05 p<0.01 Average VCM ± SD (M/sec) of the sural nerve in diabetics treated with uridine and with placebo. URIDINE PLACEBO Student Anova Pre-basal 32.6+3.0 32.7+3.2 N.S. N.S. Basal 32.8+2.0 33.0+2.5 N.S. N.S. Day 60 34.0+2.3 32.9+2.0 p<0.05 p<0.01 Day 120 37.2+2.2 33.4+2.6 p<0.005 p<0.001 Day 180 41.1+2.2 33.0+2.3 p<0.001 p<0.001 Follow-up 40.1+1.7 33.2+2.2 p<0.005 p<0.001 Average amplitude ± SD (microV) of the SAP of the sural nerve in diabetics treated with uridine and with placebo. URIDINE PLACEBO Student Anova Pre-basal 4.5+1.9 4.7+2.3 N.S. N.S. Basal 4.4+1.8 4.8+2.4 N.S. N.S. Day 60 4.9+2.0 4.6+2.1 N.S. N.S. Day 120 5.9+2.0 4.7+1.9 p<0.05 p<0.05 Day 180 7.0+2.4 4.7+2.2 p<0.001 p<0.01 Follow-up 6.7+1.7 4.9+2.2 p<0.005 p<0.01 SAP = potential of sensorial action Conclusions The results reported above show that uridine is capable of reducing the entity of complications in diabetes mellitus in a group of patients treated for 6 months with the drug. The study was performed using a double-blind test and the results are derived from objective measures. It can thus be concluded that uridine, probably by means of the biosynthesis of glycogen within the cells, limits the damage caused by high levels of glucose, and can thus be used in the treatment of peripheral disturbances in diabetes, such as retinopathy, vasculopathy, etc. The daily dose can vary between 500 and 2000 mg per day of uridine taken orally and the dose can be administered using the normal pharmaceutical forms.

Claims: Use of uridine with exclusion of a combination thereof with cytidine for the manufacture of a medicament for the pharmacological treatment of complications produced by diabetes mellitus in the peripheral nervous system or in the peripheral vascular system. Use of uridine as claimed in claim 1, in which said complications of the peripheral nervous system is muscular neuropathy or retinopathy. Use of uridine as claimed in claim 1 for manufacture of a medicament in orally administrable form for the pharmacological treatment of complications produced by diabetes mellitus in the peripheral nervous system or in the peripheral vascular system.

Inventors: DE LUCA GIOVANNA, DI STAZIO GIOVANNI, MATERAZZI MARIO, POLITI VINCENZO, DE LUCA, GIOVANNA, DI STAZIO, GIOVANNI, MATERAZZI, MARIO, POLITI, VINCENZO
Applicants: POLIFARMA SPA, POLIFARMA S.P.A.
Application year: 1991
Main classification: A61K31
IPC codes: A61K31, A61P3, A61P21, A61P27, A61P9, C07H19