ANTIBACTERIAL PENEM COMPOUNDS

Abstract: Antibiotic penem coumpounds are represented by formula (I), wherein R represents a physiologically-hydrolyzable, ester-forming group. The physiologically hydrolyzable, ester-forming group useful in the penem compound of the present invention means a group which can be removed easily by in vivo hydrolysis, such as an acetyloxymethyl, 1-(acetyloxy)ethyl, pivaloyloxymethyl, 1-(ethoxycarbonyloxy)ethyl, 1-(isopropyloxycarbonyloxy)-ethyl, 1-(cyclohexyloxycarbonyloxy)ethyl or 3-phthalidyl group. Antibiotic compositions for oral administration are also described.

Description: DESCRIPTION ANTIBACTERIAL PENEM COMPOUNDS. Technical Field The present invention relates to penem compounds, and more specifically to penem compounds which are ex¬ pected to find clinical utility as promising antibiotics. Background Art The present inventors previously found that a group of penem compounds represented by the following formula: wherein R- ^ is a hydrogen atom or allyl group, -A- represents an oxygen or methylene group, and -B- represents a methylene, ethylene or carbonyl group, and their salts have excellent antibacterial activities on both gram-positive and gram-negative, aerobic or anaerobic bacteria and have already filed a patent ap- plication thereon (Japanese Patent Laid-Open No. 207387/1986) . High safety of these compounds has been confirmed by a safety test in which laboratory animals were used. Their development as medical drugs is now expected. In the meantime, it has been found by the study on the correlation between structure and antibacterial activities of these compounds [J. Antibiotics, 41, 1685 (1988)] that, among 2-substituents of penem, (R)-2- tetrahydrofuryl group provides the highest antibac¬ terial activities while (S)-2-tetrahydrofuryl group, a diastereomer at its 2-side chain group, and (R) or (s)- 3-tetrahydrofuryl group, a position isomer, provide weaker activities particularly against gram-negative bacteria. From these reasons, compounds represented by the following formula (II) : ( π ) wherein R 2 represents a hydrogen atom or a group capable of forming a pharmaceutically-acceptable salt, have drawn interests as antibiotics. These compounds are also interested in that they do not require any special chemical modification for oral absorption and they themselves can be developed as both injections and oral drugs. Namely, the bioavailability of the above- described compounds per se in laboratory animal (rats) has been found by no means inferior to commercial drugs which are used clinically. However, from the viewpoint of safety and econo- my, further enhancement of their bioavailability upon oral administration is apparently more advantageous. As far as the above compounds are concerned, there is still a room for further improvement in this regard. Regarding improvements in the absorption upon oral administration, extensive studies have been con¬ ducted on penicillin and cephalosporin antibiotics so that many of these antibiotics are used as curative medicines. There are, however, only a few study reports of this type on penem and carbapenem anti- biotics [J. Antibiotics, 36, 983, (1983); Japanese Patent Laid-Open No. 6728/1990] . It has therefore been interested in determining whether or not the approaches used for penicillin and cephalosporin antibiotics are equally applicable to penem compounds. Disclosure of Invention The present inventors have carried out an ex¬ tensive investigation on the compounds (II) with a view toward making an improvement in their bioavailability. As a result, it has been found that protection of their carboxyl group with an ester-forming group which can be hydrolyzed easily in vivo can significantly improve their bioavailability, leading to the completion of the present invention. Namely, the present invention provides a penem compound represented by the following formula (I) : ( I • wherein R represents a physiologically-hydrolyzable, ester-forming group. The physiologically hydrolyzable, ester-forming group useful in the penem compound of the present in¬ vention means a group which can be removed easily by in vivo hydrolysis. Its examples include an acetyloxy¬ methyl group, l-(acetyloxy)ethyl group, pivaloyloxy- methyl group, l-(ethoxycarbonyloxy)ethyl group, 1- (isopropyloxycarbonyloxy)ethyl group, l-(cyclohexyloxy- carbonyloxy)ethyl group and 3-phthalidyl group. When these groups contain an asymmetric carbon atom, it is preferable that they are optically active. Best Mode for Carrying Out the Invention The penem compound (I) of the present invention can be synthesized, for example, by reacting a halogenated alkyl compound (IV) to a penem compound (II 1 ) in accordance with the following formula: ( II ' ) wherein X represents a halogen atom, R 3 represents a hydrogen or alkali metal atom or an amino residuum, and R has the same meaning as defined above. When R 3 in the compound (II*) is an alkali metal atom or an amino residuum in the above reaction, the target product can be obtained by stirring the compound (II 1 ) together with the halogenated alkyl compound (IV) in an organic solvent. When R 3 in the compound (II 1 ) is a hydrogen atom on the other hand, it is firstly reacted with an alkali metal hydroxide, an alkali metal salt or an amine com¬ pound in an organic solvent to form a salt, and the mixture is then reacted with the halogenated alkyl com¬ pound (IV) , whereby the target compound can be synthe¬ sized. The halogenated alkyl compound represented by the formula (IV) can efficiently esterify the carboxyl group of the compound (II 1 ) with the group R to produce the target compound of the formula (I) . Examples of the compound (IV) include those employed for the prepa- ration of prodrugs of the penicillin or cephalosporin type. Illustrated more specifically, they may be com¬ pounds of the formula (IV) in which R is a physio- logically-hydrolyzable, ester-forming group such as an acetyloxymethyl group, l-(acetyloxy)ethyl group, pivaloyloxymethyl group, l-(ethoxycarbonyloxy)ethyl group, l-(isopropyloxycarbonyloxy)ethyl group, 1- (cyclohexyloxycarbonyloxy)ethyl group or 3-phthalidyl group and X is a chlorine, bromine or iodine atom. No particular limitation is imposed on the alkali metal insofar as it forms a salt with the compound (II'). Examples of the alkali metal include lithium, sodium and potassium. Examples of their hydroxides and salts include sodium hydroxide, potassium hydroxide, sodium bicarbonate, sodium carbonate, potassium bicar¬ bonate and potassium carbonate. Exemplary amine com¬ pounds include ammonia, triethylamine, and diisopropyl- ethylamine. No particular limitation is imposed on the reac- tion solvent. Examples of the reaction solvent include aromatic hydrocarbons such as toluene and xylene, aliphatic hydrocarbons such as pentane and hexane, halogenated alkyls such as methylene chloride and c. ^roform, halogenated aryls such as chlorobenzenes, ketones such as acetone and methyl ethyl ketone, nitriles such as acetonitrile and propionitrile, amides such as dimethyIformamide, sulfoxides such as dimethyl sulfoxide, ethers such as diethyl ether and tetra- hydrofuran, and alcohols such as isopropanol and t- butanol. They can be used either singly or in combina¬ tion. The reaction may be carried out at room tempera¬ ture or, in some instances, under heating below 80"C. The reaction time is generally 1-48 hours although it varies depending on the halogenated alkyl compound to be used. The penem compound (I) obtained as a reaction product as described above may be used as is but, in general, is purified, as needed, by a method such as column chromatography or recrystallization for use as a medicine. For oral, parenteral or external administration, the compounds according to the present invention can be formulated as antibiotics in a manner known per se in the art. Although the dosage of each penem derivative of the present invention varies depending on many factors, the typical daily dosage ranges from 50 mg to 3 g for standard adults with the administration of 100 mg to 2 g in divided portions being preferred. In general, the above dosage will be administered in the form of a dosage unit which contains an appropriate amount of the active ingredient and a suitable, physiologically- acceptable carrier or extender. For oral administration, tablets or capsules can be used. They may contain - together with the active ingredient - an extender, e.g., lactose, glucose, sucrose, mannitol, sorbitol or cellulose, and a lubricant, e.g., talc, stearic acid or a stearate salt. Tablets may additionally contain a binder, for example, hydroxypropyl cellulose or starch. The compounds according to the present invention can be used not only for men but also for animals. The present invention will hereinafter be de- scribed more specifically by the following examples. It should however be borne in mind that the present in¬ vention is not limited at all by these examples. Example 1 Acetyloxymethyl (5R,6S)-6-[(R)-1-hydroxyethyl]- 2-[ (R)-2-tetrahydrofuryl) ]penem-3-carboxylate (Compound 2) : To the mixture of sodium (5R,6S)-6-[ (R)-l- hydroxyethyl]-2-[ (R)-2-tetrahydrofuryl)penem-3- carboxylate 2.5 hydrate (Compound 1, 3.52 g) and N,N- dimethylformamide (40 ml) , the mixture of acetyloxy¬ methyl bromide (1.84 g) and N,N-dimethylformamide (7 ml) was added dropwise under ice-cooling and stir¬ ring, followed by stirring at room temperature for fur¬ ther 2 hours. The reaction mixture was then added with ethyl acetate (200 ml) and washed twice with water (200 ml) . The ethyl acetate layer was dried and con¬ centrated. The residue was separated and purified by passing it through a silica gel column, whereby 2.24 g of the title compound were obtained. Example 2 Phthalidyl (5R,6S)-6-[ (R)-1-hydroxyethyl]- 2-[(R)-2-tetrahydrofuryl)penem-3-carboxylate (Compound 8, Compound 9) The mixture of sodium (5R,6S)-6-[ (R)-l-hydroxy- ethylJ^-C (R)-2-tetrahydrofuryl)penem-3-carboxylate 2.5 hydrate (Compound 1, 3 g) , bromophthalide (3 g) and dimethyl sulfoxide (30 ml) was stirred at room tempera¬ ture for 10 minutes. The reaction mixture was added with water, followed by extraction with ethyl acetate. The ethyl acetate layer was washed with water and the resulting organic layer was dried. Then, the solvent was diluted off. The residue thus obtained was sepa¬ rated and purified by passing it through a silica gel column, whereby the title compound was obtained. At that time, diastereomers relative to the asymmetric carbon atom of the phthalidyl group were separated. The less polar compound was designated as "Compound 8", while the more polar compound was designated as "Com- pound 9". The yields of those compounds were 1 g and 1.3 g, respectively. Example 3 1-(Cyclohexyloxycarbonyloxy)ethyl (5R,6S)-6-[ (R)- 1-hydroxyethyl]-2-[ (R)-2-tetrahydrofuryl)penem-3- carboxylate (Compound 7) : Sodium (5R,6S)-6-[ (R)-l-hydroxyethyl]-2-[ (R)-2- tetrahydrofuryl)penem-3-carboxylate 2.5 hydrate (Com¬ pound 1, 3.52 g) was added to the mixture of 1-iodo- ethyl cyclohexylcarbonate (3.3 g) and N,N-dimethyl- formamide (60 ml) . The resultant mixture was heated at room temperature for one hour, followed by the addition of ethyl acetate (300 ml) . The reaction mixture thus obtained was washed with water. The ethyl acetate layer was dried and then concentrated. The residue was separated and purified by passing it through a silica gel column, whereby 1.38 g of the title compound was obtained. Examples 4-7 In each example, sodium (5R,6S)-6-[ (R)-1-hydroxy- ethy1]-2-[ (R)-2-tetrahydrofuryl]penem-3-carboxylate 2.5 hydrate (Compound 1) and the corresponding halogenated alkyl compound, which is shown in Table 1, were pro¬ cessed in a similar manner to Example 3, whereby the penem compound of the present invention was obtained. Incidentally, physical properties of the compounds ob¬ tained in each example are shown in Table 2. Table 1 Table 2 (1) Table 2 (2) The bioavailability of certain compounds (I) of the present invention was tested relying upon their recovery rates in urine. Each test compound (30 μmole/kg) was orally ad¬ ministered to SD strain rats (three male rats per group) . Urine was collected over 6 hours from the ad¬ ministration, and the recovery rate of the correspond¬ ing compound present in the urine was determined by bioassay. The results are shown below. Table 3 As is apparent from the results, the compounds (I) of the present invention showed the higher urinary recovery rates compared with the penem compound (II) . This indicates that the bioavailability has been sig- nificantly improved by the esterification of the car- boxyl group of the penem compound (II) with the ester- forming groups in accordance with the present inven¬ tion. Like Compound 7, there are however some compounds which show almost no change in the recovery rate. Even among other compounds, there are differences in the rate of improvement, ranging from three times to six times. As a result, it has been found that all the ester side chains effective in drugs such as penicillin and cephalosporin are not effective but improvement in bioavailability may be brought about depending upon the combination of the penem compound, that is the mother compound, and an ester-side-chain-forming compound. Preparation Examples In each of the following preparation examples, the active ingredient may be, for example, Compound 6 or an equivalent amount of any one of the other com¬ pounds of the present invention. Preparation 1 Capsules Ingredient No. Ingredient mg/capsule 1 Invention compound 150 2 Lactose 20 3 Magnesium stearate 4 (Total) 174 mg (Production procedures) Ingredients 1 and 2 were combined together in a suitable mixer, to which Ingredient 3 was added, followed by further mixing. The resultant mixture was filled using a capsule filling machine. Preparation 2 Tablets Ingredient No. Ingredient mg/tablet 1 Invention compound 150 2 Crystalline cellulose 50 3 Calcium carboxymethylcellulose 10 4 Magnesium stearate 4 (Total) 214 mg (Production procedures) Ingredients 1-3 were combined together in a suitable mixer, to which Ingredient 4 was added, followed by mixing for additional several minutes. The resultant mixture was compressed into a predetermined size and weight by a tablet machine. Industrial Applicability As has been described above, the compounds of the present invention exhibit excellent bioavailability so that they can be advantageously used as oral anti¬ biotics.

Claims: ( I ) wherein R represents a physiologically-hydrolyzable, ester-forming group. 2. The compound according to claim 1, wherein R represents an acetyloxymethyl group. 3. The compound according to claim 1, wherein R represents a l-(acetyloxy)ethyl group. 4. The compound according to claim 1, wherein R represents a pivaloyloxymethyl group. 5. The compound according to claim 1, wherein R represents a l-(ethoxycarbonyloxy)ethyl group. 6. The compound according to claim 1, wherein R represents a l-(isopropyloxycarbonyloxy)ethyl group. 7. The compound according to claim 1, wherein R represents a l-(cyclohexyloxycarbonyloxy)ethyl group. 8. The compound according to claim 1, wherein R represents a 3-phthalidyl group. 9. The compound according to any one of claims 1, 3, 5, 6, 7 and 8, wherein R is an optically active group. 10. An antibiotic composition for oral adminis¬ tration, comprising an effective amount of a compound of the following formula (I) : ( I ) wherein R represents a physiologically-hydrolyzable, ester-forming group, and a pharmaceutically-acceptable carrier.

Inventors: ISHIGURO MASAJI, IWATA HIROMITSU, NATAKSUKA TAKASHI, TANAKA RIE, ISHIGURO, MASAJI, IWATA, HIROMITSU, NATAKSUKA, TAKASHI, TANAKA, RIE
Applicants: SUNTORY LTD, SUNTORY LIMITED
Application year: 1991
Main classification: A61K31
IPC codes: A61K31, A61P31, C07D499