LIPOSOMAL COMPOSITIONS

Abstract: Liposomal Cromolyn formulations comprising sodium cromoglycate and a lipid or lipid mixture are disclosed. The lipids employed in the cromolyn liposomal formulations of this invention include cholesterol neutralized cholesterol hydrogen succinate (cholesterol hemisuccinate or ''CHS''); saturated phospholipids such as dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), hydrogenated soy phosphatidylcholine (HSPC); and cholesterol (Chol), or mixtures of same.

Description: LIPOSOMAL COMPOSITIONS CROSS REFERENCE TO COPENDING U.S. PATENT APPLICATIONS This application is a continuation-in-part of copending U.S. Patent Application Serial Number 429,278, filed October 30, 1989. Background of the Invention The present invention is directed to liposomal cromolyn formulations comprising cromolyn and lipids. This invention specifically discloses formulations wherein the lipid portion of the liposome comprises lipids such as neutralized cholesterol hydrogen succinate known as cholesterol hemisuccinate (CHS), saturated phospholipids such as hydrogenated soy phosphatidylcholine (HSPC), dipalmitoylphosphatidylcholine (DPPC) or lipid mixtures such as a HSPC-cholesterol, DPPC-cholesterol or dimyristoylphosphatidylcholine (DMPC)-cholesterol. The term cromolyn as used herein includes all structural analogs and functional derivatives of cromolyn as disclosed in U.S. Patent No. 3,419,578 which is hereby incorporated by reference. Functional derivatives of cromolyn include its salts, esters and amides, in particular the disodium salt of cromolyn e.g. disodium cromoglycate or cromolyn sodium. Cromolyn, whose chemical names include 5,5' -[(2-Hydroxy-l,3- propanediyl)bis-(oxy)]bis[4-oxo-4H-l-benzopyran-2-carboxylic acid] ; 5,5' -[(2-hydroxytrimethylene)dioxy]bis(4-oxo-4H-l-benzopyran-2-carboxylic acid); 5,5' -(2-hydroxytrimethylenedioxy)bis(4-oxochromene-2-carboxylic acid); 1,3-bis(2-carboxychromon-5-yloxy)-2-hydroxypropane; l,3-di(2-carboxy-oxochromen-5-yloxy)propan-2-ol; and cromoglycic acid, has the following chemical structure: Its method of preparation is described in U.S. Patent No. 3,419,578 previously incorporated by reference. Cromolyn is known to be useful for the treatment of allergic conditions, e.g. asthma, hay fever and conjunctivitis of allergic origin, e.g. vernal kerato conjunctivitis and allergic rhinitis. Other disclosed uses of disodium cromoglycate include the treatment of viral infections, the treatment of gastro-intestinal diseases e.g. ulcerative colitis and food allergies and the treatment of skin conditions, e.g. chronic dermatoses such as eczemas, psoriasis, dermatitis and chronic skin ulcers. Liposomes are completely closed lipid bilayer membranes containing an entrapped aqueous volume. Liposomes may be unilamellar vesicles (possessing a single bilayer membrane) or multilamellar vesicles (onion-like structures characterized by multiple membrane bilayers, each separated from the next by an aqueous layer). The bilayer is composed of two lipid monolayers having a hydrophobic "tail" region and a hydrophilic "head" region. The structure of the membrane bilayer is such that the hydrophobic (nonpolar) "tails" of the lipid monolayers orient toward the center of the bilayer while the hydrophilic "head" orient towards the aqueous phase. The original liposome preparation of Bangham, et al. (J. Mol. Biol.. 1965, 12:238-252) involves suspending phospholipids in an organic solvent which is then evaporated to dryness leaving a phospholipid film on the reaction vessel. Next, an appropriate amount of aqueous phase is added, the mixture is allowed ,to "swell," and the resulting liposomes which consist of multilamellar vesicles (MLVs) are dispersed by mechanical means. This technique provides the basis for the development of the small sonicated unilamellar vesicles described by Papahadjopoulos et al. (Biochim. Biophvs. Acta.. 1968, 135:624-638), and large unilamellar vesicles. Unilamellar vesicles may be produced using an extrusion apparatus by a method described in Cullis et al., PCT Application No. 087/00238, published January 16, 1986, entitled "Extrusion Technique for Producing Unilamellar Vesicles" incorporated herein by reference. Vesicles made by this technique, called LUVETS, are extruded under pressure through a membrane filter. Vesicles may also be made by an extrusion technique through a 200 run filter. Such vesicles are known as VET„ 0( .S. Another class of liposomes that may be used in the present invention are those characterized as having substantially equal lamellar solute distribution. This class of liposomes is denominated as stable plurilamellar vesicles (SPLV) as defined in U.S. Patent No. 4,522,803 to Lenk, et al., monophasic vesicles as described in U.S. Patent No. 4,588,579 to Fountain, et al. and frozen and thawed multilamellar vesicles (FATMLV) wherein the vesicles are exposed to at least one freeze and thaw cycle; this procedure is described in Bally et al., PCT Publication No. 087/00043, January 15, 1987, entitled "Multilamellar Liposomes Having Improved Trapping Efficiencies". Cromolyn (e.g., sodium cromoglycate) liposomal formulations are known in the art. European Patent Publication No. 0 084 898 published August 3, 1983, discloses pharmaceutical compositions comprising liposomes and sodium cromoglycate for treating asthma, hay fever and vernal kerato conjunctivitis. However, liposomal cromolyn compositions having the formulations of the instant invention such as neutral pH CHS lipids or DMPC-Chol or HSPC-Chol lipid mixtures are new. Summary of the Invention The present invention is directed to liposomal compositions of cromolyn and lipid. The lipid component of the inventive compositions may be cholesterol hydrogen succinate (CHS); saturated phospholipids such as for example hydrogenated soy phosphatidylcholine (HSPC), dipalmitoyl phosphatidylcholine (DPPC), hydrogenated soy phosphatidylcholine (HSPC); dimyristoylphosphatidylcholine (DMPC), or a hydrogenated soy phosphatidylcholine and cholesterol mixture (HSPC/Chol); or a dimyristoylphosphatidylcholine and cholesterol mixture (DMPC/Chol) or a dipalmitoyl phosphatidylcholine and cholesterol mixture (DPPC/Chol). The inventive liposomal compositions may be incorporated into a pharmaceutical composition by combining or admixing them with a pharmaceutically carrier or diluent. ThB liposomal cromolyn compositions of the present invention are especially useful for treating allergic conjunctivitis or allergic rhinitis. A preferred CHS cromolyn liposome formulation additionally contains disodium EDTA and phosphate buffer to a pH around about pH 7.0. It may also include benzalkonium chloride and phenylethyl alcohol. A preferred cromolyn liposome composition containing a saturated phospholipid and cholesterol also contains phosphate buffer to a pH around about neutrality, e.g., pH 7.0. Such composition may also contain disodium EDTA. The phosphate buffer is preferably sodium phosphate monobasic and sodium phosphate dibasic. The ratio of cromolyn to lipid in such compositions ranges from about 0.005 to about 20.0, more preferably from about 0.071 to about 0.667, and most preferably is about 0.2. The saturated lipid of the invention is dimyristoylphosphatidyl¬ choline (DMPC), dipalmitoylphosphatidylcholine (DPPC), or hydrogenated soy phosphatidylcholine (HSPC). The liposome composition may contain a preservative, which can be phenylmercurie acetate, benzalkonium chloride, or chlorbutanol. It may also be phenylmercuric acetate. The preservatives may be employed alone or in combination, and the preparations may also contain methylparaben and propylparaben. When the saturated lipid is dimyristoylphosphatidylcholine (DMPC), the formulation may also contain phenylmercuric acetate, and benzalkonium chloride or phenylethyl alcohol. Alternatively, the formulation of DMPC/cholesterol may contain benzalkonium chloride and thimersol. Pharmaceutical composition comprising the liposome composition of any of the above formulations are also contamplated, wherein the composition comprises a pharmaceutically acceptable carrier or diluent. Brief Description of the Drawing Figure 1 is a schematic for the preparation of cromolyn liposomes by aseptic process. Figure 2 is a graphic presentation of the tissue concentration (mg Cromolyn/g or ml tissue) of the Cromolyn Free Drug over time (also found in Table 1). Detailed Description of the Invention The lipids which can be used in the liposome formulations of the present invention are the unsaturated and saturated phospholipids such as phosphatidylcholine (PC), hydrogenated soy phosphatidylcholine (HSPC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidic acid (PA), phosphatidylinositol (PI), sphingomyelin (SPM), and the like, alone or in combination. Preferred phospholipids include dimyristoylphosphatidylcholine (DMPC), dipalmitoyl phosphatidylcholine (DPPC), and hydrogenated soy phosphatidylcholine (HSPC). These lipids can be used alone or in combination with cholesterol. The phospholipids can be synthetic or derived from natural sources such as egg or soy. The liposomes can also contain other steroid components such as derivatives of cholesterol, coprostanol, cholestanol, or cholestane, and combinations of PC and cholesterol. They may also or alternatively contain organic acid derivatives of sterols such as cholesterol hemisuccinate (CHS) especially neutral-pH cholesterol hemisuccinate, and the like. CHS containing liposomes and their salt forms may generally be prepared by any method known in the art for preparing liposomes containing these sterols. In particular, see the procedures of Janoff, et al., U.S. Pat. No. 4,721,612 issued January 26, 1988, entitled "Steroidal Liposomes", incorporated herein by reference. Derivatized sterols of the invention have a general structure wherein a core molecule (A) comprises a sterol, a steroid, a bile acid or an intermediate of sterol metabolism; a chemical bridge (B) such as an ester, ether, sulfide (thioether), alkyl (methylene, ethylene, propylene, butylene, etc.), amide, amine, phosphonate, thioester, sulfonate and disulfide between the core molecule (A) and an anchor molecule (C); C is selected from among linear branched or cyclic structures terminating in a charged or hydrophilic group (D); (D) the proximal anchor, is a hydrophilic or charged group, preferably a charged group, selected from among a carboxylate, carbonate, phosphate, sulfonate, sulfate, borate, protonated amine, quarternary amine, imidazole, sulfonium, pyrrolidone, pyrrole and pyridinium; and a counter ion (E) to said group (D) when (D) is charged, selected from among an acetate, sulfate, sulfonate, phosphate, arsenate, borate, nitrate, chlorate, a metal ion, charged amine, including a quarternary amine, imidazole, sulfonium and diazonium salt, among others, including larger molecules, for example, amino acids, EDTA, TRIS, choline, peptides, nucleosides, nucleotides and oligo and polynucleotides, among others. The liposomes can comprise any of the abovenamed lipids alone or in combination. A preferable lipid combination for the liposome compositions of the invention is DMPC/cholesterol or HSPC/cholesterol, in a 16:1 to about a 1:1 weight ratio, more preferably about a 10.0 : 2.4 %w/v ratio. The cromolyn to lipid weight ratios of the liposomal compositions of the present invention range about .005 (0.1 %w/v drug, 20.0 %w/v lipid) to about 20.0 (10 %w/v drug, 0.5 %w/v lipid), more preferably from about .071 (1.0 %w/v drug, 14.0 %w/v lipid) to about .667 (4.0 %w/v drug, 6.0 %w/v lipid), most preferably about .2 (2.0 %w/v drug, 10.0 %w/v lipid). The liposome-cromolyn formulations can additionally contain one or more antimicrobial preservative solutions, such as sorbic acid, p-hydroxybenzoic acid methyl, ethyl, butyl or propyl esters, benzyl alcohol, chlorbutanol, benzalkonium chloride (BAK), phenethyl alcohol, and the mercurial preservatives such as phenylmercuric acetate. Other preservatives include boric acid, benzethonium chloride, butylparaben, cetylpyridinium chloride, chlorhexidine digluconate, methylparaben, propylparaben and thimersol. The preservative is preferably present in the final preparation at about 0.01-0.1% (w/v), most preferably 0.05%. The amount of preservative needed can be determined by its performance in the USP Antimicrobial Effectiveness test (AME), The United States Pharmacopeia The National Formulary, The United States Pharmacopeial Convention, Inc., Rockville, MD, which is incorporated herein by reference, and by its structural and chemical compatibility with other components in the preparation. The preservative solution may also comprise enhancer substances such as ethylenediamine tetraacetic acid (EDTA) at about 0.01-0.1% (w/v), preferably 0.05% (w/v). Preferably in the present invention, sorbic acid: EDTA (1 : 1) is used. The liposome-cromolyn formulations of the invention can additionally contain an antioxidant, such as for example butylated hydroxytoluene (BHT). Any aqueous solutions such as for example aqueous buffers can be used in the practice of the instant invention. Especially preferable are those pharmaceutically acceptable solutions such as physiological saline, citrate, carbonate, phosphate or tris(hydroxymethyl)aminomethane (Tris). The concentrations of these solutions will depend upon other components of the formulation and their concentrations, which solutions will result in a tonicity as close to physiological as possible; such being within the knowledge of one having ordinary skill in the formulations art. The solutions preferably employed in the present invention are phosphate or citrate. Where buffer capacity near about pH 7.0 is required, phosphate buffer was employed. The preferred phosphate buffers are sodium phosphate, monobasic and sodium phosphate, dibasic. Most preferably these buffers are employed together in amounts which will adjust the final pH of the liposomal cromolyn compositions to about pH 7.0. Citrate was selected for buffering at pH 5.5. These two pH values fall into the preferred range for cromolyn. Sodium chloride can be used to adjust tonicity. Compounds that are hydrophilic polymer viscosity enhancers such as polyvinyl alcohol, polyvinyl pyrolidone or hydroxypropyl methyl cellulose, which also enhances the retention of liposomes on the tissues can be used. These compounds are used in about a 0.05-1.5% (w/v) concentration of final preparation. The cromolyn liposomes of the invention can be size reduced or homogenized by a variety of methods including for example the Continuous Size Reduction process disclosed in commonly assigned copending U.S. Patent Application SN 036,980, filed April 16, 1987, and incorporated herein by reference. In this technique, a liposome solution is passed under pressure a multiple of times (preferably about 10 times) through a stainless steel filter having a nominal pore size of about 500 nm. Alternatively, the liposomes can be extruded by passage through a uniform-pore size filter according to the LUVET method, described hereinabove, disclosed in Cullis et al., PCT Publication No. 87/00238, January 16, 1986. Alternatively, the liposomes of the invention can be homogenized using an apparatus such as the Mantin-Gaulin homogenizer or the Silverson homogenizer. 5 The liposomes of the present invention may be dehydrated thereby enabling storage for extended periods of time until use. Standard freeze-drying equipment or equivalent apparatus may be used to lyophilize the liposomes. Liposomes may also be dehydrated simply 10 ky placing them under reduced pressure and allowing the suspending solution to evaporate. Alternatively, the liposomes and their surrounding medium may be frozen in liquid nitrogen prior to dehydration. Such dehydration may be performed in the presence of one or more protectants such as protective sugars, according to the j ς process of Janoff et al., U.S. Patent No. 4,880,635, issued November 14, 1989, and PCT publication No. W086/01103, published February 27, 1986, both of which are incorporated herein by reference. During preparation of the liposomes, organic solvents may be 20 used to solubilize the lipids. Suitable organic solvents are those with a variety of polarities and dielectric properties, which solubilize the lipids, and include but are not limited to halogenated, aliphatic, cycloaliphatic, or aromatic-aliphatic hydrocarbons, such as benzene, chloroform, methylene chloride, or 25 alcohols, such as methanol, ethanol, and solvent mixtures such as benzene:methanol (70:30 v/v). As a result, solutions (mixtures in which the lipids and other components are uniformly distributed throughout) containing the lipids are formed. Solvents are generally chosen on the basis of their biocompatability, low 3 Q toxicity, and solubilization abilities. For the purposes of the instant invention, the organic solvent methylene chloride is particularly preferred. Examples 2-4 hereinbelow disclose methods for forming the 35 liposomal cromolyn formulations of the invention. For an aseptic process, a schematic diagram of one method of preparation of the liposomal cromolyn of the invention is presented in Figure 1. In one method of making the liposome-cromolyn compositions of the present invention, lipids are dissolved in organic solvent while cromolyn (sodium cromoglycate) is dissolved in aqueous solution such as for example water. The organic and aqueous phases are transferred to a round bottom flask and the solvent removed by vacuum evaporation. The resulting preparation can then be homogenized using any device suitable for such a process, e.g., a Silverson homogenizer. If viscosity enhancers, antioxidants or antimicrobial agents are employed, they can be admixed with the aqueous solution of cromolyn (sodium cromoglycate) prior to its admixture with the organic phase. Water insoluble components (e.g., such as the antioxidant) can be admixed with the lipid phase in organic solvent). As examples, formulations of the lipid-cromolyn compositions can comprise for example HSPC : cholesterol in about 10:2.4 to about a 20:4.8 %w/v. DMPC : cholesterol can be used in about a 10:2.4 %w/v ratio. DPPC : cholesterol can be used in about a 10:2.4 %w/v concentration, and EPC can be used either alone or in a 10:30 to 20:60 %w/v concentration with cholesterol. CHS can be used alone in the formation of cromolyn - CHS liposomes of the invention. As a further example of the methods of the invention, where DMPC-cholesterol is employed as the lipid phase, the lipid used can comprise DMPC and cholesterol in the proportions recited above, admixed in organic solvent. The aqueous phase can comprise 2.0 %w/v sodium cromoglycate. The composition can also contain preservatives such as for example, benzalkonium chloride 0.01 % w/v, and chlorobutanol 0.5 %w/v, and enhancers such as disodium edetate (EDTA) 0.013 %w/v. Yet another formulation for liposomal cromolyn is HSPC-cholesterol. HSPC can be present at 10 %w/v, cholesterol at 2.4 %w/v, and the drug present at 2.0 %w/v cromolyn. Preservatives may or may not be present in the compositions. Anti-oxidants can also be present, for example, butylated hydroxytoluene at 0.001 %w/v. Still another liposomal cromolyn formulation of the invention is CHS which can be present at 10.0 %w/v, with cromolyn at 2.0 %w/v. Preservatives can also be present in these compositions. 5 Anti-oxidants such as butylated hydroxytoluene can also be employed for example at 0.001 %w/v. Examples of formulations of the invention are shown in appended Tables 3 through 9 and 11 through 20. These liposome formulations J ^ Q are made by the general methods as recited hereinabove and more specifically by the methods disclosed in Examples 2, 3 and 4 or Figure 1 (for an aseptic batch process) of the invention as recited hereinbelow. Formulations of the invention were tested for anitmicrobial effectiveness by the USP National Formulary methods. 15 Tables 11, 12 and 20 also show formulations of the invention that did not pass the USP Antimicrobial Effectiveness Testing (referenced hereinabove), and thus are not preferable for use in the present invention. 20 A therapeutically effective amount of the compositions herein will be understood to mean a sufficient amount to achieve a physical or physiological response over a period of time. The therapeutically effective amount of a given pharmacological agent will vary with the purpose of the administration, the 25 particularities of the recipient and other factors, especially those related to sustained release, well known in the art. The liposomes of the present invention can be used therapeutically in mammals, including man, in the treatment of 30 conditions which can be ameliorated by cromolyn in its bioactive form. These conditions include but are not limited to allergic conjunctivitis and allergic rhinitis. In such a use as a pharmaceutical composition, the liposomes of the invention can be admixed with a pharmaceutically acceptable carrier or diluent. 35 The mode of administration of the preparation may determine the sites and cells in the organism to which the compound will be delivered. Liposomes can be administered alone but will generally be administered in admixture with a pharmaceutical carrier selected with regard to the intended route of administration and standard pharmaceutical practice. The preparations may be injected parenterally, for example, intra-arterially, subcutaneously, intramuscularly or intravenously. The preparations may also be administered via oral, subcutaneous, or intramuscular routes, or by inhalation. For parenteral administration, they can be used, for example, in the form of a sterile aqueous solution which may contain other solutes, such as for example, salts or glucose or any other solutes which are biocompatible. Other uses, depending upon the particular properties of the preparation, may be envisioned by those skilled in the art. A preferred mode of administration is via eye drops in a form such that the eye drops can be administered using a standard ophthalamic dropper or dropper bottle. Another preferred mode of administration is via nasal spray or a nebuliser. For administration to humans in the palliative or therapeutic treatment of disease states, the prescribing physician will ultimately determine the appropriate dosage for a given human subject, and this can be expected to vary according to the age, weight, and response of the individual as well as the nature and severity of the patient's disease. The dosage of the drug in liposomal form will generally be about that necessary for the therapeutic treatment of the disease state or condition. In some cases, however, it may be necessary to administer doses outside these limits. The following examples are given for purposes of illustration only and not by way of limitation on the scope of the invention. EXAMPLE 1 A. Preparation of test article. The sodium cromoglycate free drug test article used in the ocular biodistribution studies (Examples 1, 2 and 3) was prepared according to the following formula: Sodium cromoglycate 2.0 %w/v EDTA-disodium 0.01 %w/v Benzalkonium Chloride 0.01 %w/v Phenylethyl Alcohol 0.4 %w/v Sterile Water qs pH 5.4 14 B. The [propane-2- C]cromolyn sodium used in the biodistribution studies was prepared as follows: 14 l,3-dichloro[2- C]propan-2-ol was reacted with 2,6-dihydroxyacetophenone to give 1,3-bis (2-acetyl-3-hydroxy 14 phenoxy)-2-hydroxy[2- C]propane. This was treated with base and diethyl oxalate followed by acid catalysed cyclisation to give 14 diethyl [propane-2- C]cromolyn. Hydrolysis with sodium hydroxide gave the final product which was purified by crystallisation from water acetone. Specification and Handling of Experimental Animals and Tissues. l. Description of Experimental Animals: Female, New Zealand white rabbits (Hare Marland) weighing 2-3 kg were used in these studies. Upon receipt each animal was assigned an identification number and examined for gross signs of general health. The animals were individually housed in standard rabbit cages and given access to food and water ad libitum. Animals were received approximately one week before initiation of the study. Prior to the study the animals were randomly assigned to treatment groups using either a random number table or! generator. Immediately before treatment- the ocular tissues were examined for signs of irritation. If irritation was observed, either another animal was substituted or the condition noted. Food was removed at the time of treatment Water was available ad libitum throughout. 2. Topical Ocular Instillation of Test Article: Animals were treated unilaterally by topical ocular instillation using a positive displacement pipet. The eyelids were gently retracted and the dose applied onto the cornea taking care to avoid touching the tissue 10 with the pipet tip. The animal was positioned such that the dose flowed into the lower cul-de-sac. After treatment the animals were returned to their cages. 3. Collection of Plasma Sample: Immediately prior to 15 euthanasia, animals were placed in a standard rabbit restrainer. A small amount of xylene was applied to an eartip to enhance blood flow. The lateral ear vein was lanced and approximately 500 ul of blood collected in a Microtainer Brand Tube with EDTA (Becton Dickinson). 20 The tubes were mixed by inversion and centrifuged in a microfuge for 10 minutes. A 200 ul aliquot of plasma was transferred to a scintillation vial for further processing of the sample. 25 4. Euthanasia: Animals were euthanized by intravenous injection of a lethal dose of T61 Euthanasia Fluid (Hoechst-Roussel) into a lateral ear vein. Death was verified by absence of respiration, heart beat, and ocular reflexes. 30 5. Dissection of Ocular Tissues. a. Removal of Aqueous Humor: Aqueous humor was removed ,c using a 1 ml disposable, plastic syringe equipped with a 5/8"-27g needle. The needle was inserted into the anterior chamber at the limbus. The fluid was gently withdrawn and transferred to a preweighed scintillation vial. b. Removal of Globe, Conjunctiva, and Nictitating Membrane: The eyelids were retracted using 6" curbed hemostats. Incisions were made at the corners of the eye to facilitate dissection of the conjunctiva. The palpebral conjunctiva and nictitating membrane were dissected free of the eyelids, muscle, and connective tissue using Wilmer-Converse conjunctiva scissors. The 10 conjunctiva and nictitating membrane were grasped with straight Kirby tissue forceps to form a sack around the globe. The sack and its contents were removed from the socket as a unit using heavy curved nucleation scissors. Excess blood was removed from the outside of 15 the sac by gentle blotting with gauze pads. c. Dissection of Conjunctiva, and Nictitating Membrane: The nictitating membrane was grasped with straight Kirby tissue forceps. The conjunctiva was cut 20 with heavy curved eye scissors along one edge of the nictitating membrane. While applying tension at the nictitating membrane, the conjunctiva was dissected away from the globe using heavy curved eye scissors. The nictitating membrane was separated from the 25 conjunctiva by continuing to cut along the remaining edges. The conjunctiva was inspected and any adherent nonconjunctival tissue removed. The samples were then transferred to preweighed scintillation vials. 30 d. Removal of Cornea and Iris-Ciliary Body: Using clean instruments, the cornea was removed by cutting along the margin using heavy curved eye scissors and light curved iris forceps. The iris-ciliary body was - c grasped with clean light curved iris forceps and removed from the globe. Adherent lens and vitreous humor were removed. The tissues were transferred to preweighed scintillation vials. Determination of Radioactivity; 1. Sample Preparation: Samples were prepared and the radioactivity determined by liquid scintillation counting in 20 ml scintillation vials of low potassium borosilcate glass capped with urea-formaldehyde caps with Poly-Seal liners (liners compatible with perchloric acid and hydrogen peroxide). Eighteen ml of Ready Safe Scintillation Cocktail for Aqueous Samples 10 (Beckman) (cocktail having both salt and water compatibility which will result in a clear single phase system), were used per sample. The radioactivity in the aqueous humor samples was 15 determined without oxidation. All other tissue types were chemically oxidized prior to scintillation counting. Immediately before use, perchloric acid (70%) and hydrogen peroxide (50%) (v/v) 20 were mixed in the ratio of 1:2 by volume. A 0.3 ml aliquot of the mixture was added to a vial which was then tightly capped and incubated at 70°C for 3-4 hr. Eighteen ml of scintillation cocktail were added when cool. 25 To determine the loss of radioactivity on oxidation, control tissues were spiked with aliquots of 14 C-sodium cromoglycate of known activity and .-_ processed as indicated above. 2. Scintillation Counting: Radioactivity was determined using a Beckman LS5801 Liquid Scintillation Counter equipped with a single label DMP program. 35 Quench correction was performed by external standardization. In order to utilize counting time more efficiently, low sample rejection was used to eliminate samples with insignificant radioactivity levels. The rejection criterion was a count rate less than twice that of background samples. The remaining samples were either counted to 2 sigma error or for 360 min. 3. Calculations and Statistics: Tissue Concentrations: Tissue concentrations were expressed as %dose/g or ml tissue and as ng Cromolyn/g or ml tissue. In the latter case, it was assumed that all radioactivity was associated with the parent compound. The methods of calculation and shown in Table 2. The calculated weight means and standard deviations are shown in Table 1. E. Results: Thirty (30) rabbits were divided into three groups of ten (10) rabbits each. Groups were sacrificed at 2, 4, or 8 hours after instillation of the test article. Tissue concentrations were expressed as %dose/g or ml tissue and as ng Cromolyn/g or ml tissue. In the latter case, it was assumed that all radioactivity was associated with the parent compounds. Because Cromolyn has been reported to be nonmetabolizable and the radiolabel was of high purity, the assumption is reasonable. A Summary of the tissue concentrations is found in Table 1 and presented graphically in Figure 2. Examination of Table 1 indicates that no detectable (ND) Cromolyn levels were found in the iris-ciliary body of the treated eye, the plasma, or the aqueous humor of the untreated eye at any time point. Significant concentrations were detected in all other tissues sampled at 2, 4 and 8 hours post-instillation. Although the number of time points examined were minimal, it appears that the sodium cromoglycate concentrations in the conjunctiva and nictitating membrane declined at a relatively slow rate between 4 and 8 hours (see Figure 2). EXAMPLE 2 Liposomal-sodium cromoglycate employing CHS liposomes was made according to the following method: The aqueous phase was prepared by dissolving 02.0 g sodium cromoglycate, .05 g sorbic acid, 0.013 g disodium EDTA and 0.71 g dibasic sodium phosphate in qs ad 100 mL of sterile water for injection. The organic phase was prepared by the suspension of 10.0 g of CHS and 0.001 g BHT in 120 mL of methylene chloride in a round bottom flask. The round bottom flask was transferred to a 40°C water bath, and the aqueous phase added thereto. While agitating vigorously, 12.3 mEq of a 10 N aqueous solution of sodium hydroxide was added , and the solvent then removed by vacuum evaporation. The volume was adjusted to 100.0 mL with sterile water for injection. EXAMPLE 3 Liposomal sodium cromoglycate liposomes were made according to the methods of Example 2, wherein the lipids used were 10.0 g DMPC and 2.4 g of cholesterol. A batch size of 100 mL was prepared employing 1.34 g of sodium phosphate dibasic, heptahydrate. The lipid phase was dissolved into 100 mL of methylene chloride, and a total of 124 mL of sterile water for injection employed. EXAMPLE 4 Liposomal sodium cromoglycate liposomes were made according to the methods of Example 2, wherein the lipids used were 10.0 g HSPC and 2.4 g cholesterol. A batch size of 100 mL was prepared employing 1.34 g of sodium phosphate, dibasic, heptahydrate and sterile water for injection. The lipid phase was dissolved into 100 mL of methylene chloride, and a final volume of 100 mL in sterile water for injection was obtained. EXAMPLE 5 A large scale batch of liposomal HSPC-cholesterol sodium cromoglycate with preservatives was made as follows: Sodium cromoglycate aqueous solution was prepared by transferring 1800 mL sterile water for injection into a 2000 mL beaker and stirring to dissolve, followed by addition of 0.3 g of disodium edetate, dihydrate. Sodium cromoglycate (40 g) was slowly added to the beaker, followed by the addition of 11 g of sodium phosphate, monobasic, monohydrate. The preservative chlorbutanol was added to the mixture (12.0 g), and the suspension stirred until all solids were dissolved, at which time 1.18 mL of a 17% w/v stock solution of benzalkonium chloride was added. The resulting aqueous solution was transferred quantitatively to a 2000 mL capacity graduated cylinder, and the volume adjusted to 2000 mL with sterile water for injection. The lipid solution was prepared by transferring 0.02 g of butylated hydroxytoluene to a three-neck round bottom flask, adding 200 g of HSPC and 48 g of cholesterol. Methylene chloride was added (4195 g) and the flask heated while stirring until all solids had dissolved. The 2000 mL of aqueous solution of sodium cromoglycate was then transferred to the flask and the flask placed in a water bath set at 45°C, and the contents stirred at 96 rpm. The flask was next placed tinder vacuum of 200 mm Hg, removing the organic solvent and thus forming liposomes. EXAMPLE 6 OCULAR IRRITATION STUDIES The degree of ocular irritation of liposomal cromolyn formulations was compared to the ocular irritation of a control saline solution. The test articles employed are described in the sub examples A, B and C which follow. A. Test System - Female, New Zealand white rabbits supplied by Hare-Marland, which had been acclimated for a minimum of one week were used. Animals were housed one per cage and given access to water and food ad libitum throughout the study. Only animals judged to be healthy on the basis of general observation and fluorescein examination of the cornea were used in the study. Animals found suitable were randomly assigned to treatment groups. Approximately 0.05 mL of the test article was instilled into the cornea of the right eye of each of the four test rabbits six times per day at hourly intervals for four days. The left eye was untreated to serve as a control. The degree of ocular irritation was scored prior to each dose and at the end of the dosing period on days 1 through 4 and once daily on days 5 through 8. Fluorescein examination was performed after the final dose on day 4. Each animal was graded for ocular lesions of the cornea, iris, conjunctiva, taking into consideration redness, chemosis and discharge, on the Draize Scale (See Table 10), at the times specified hereinabove. A The test article of 0.9% sodium chloride for injection was instilled in the right eye of the 4 test animals as described hereinabove. This test articles was very well tolerated and no treatment related effects were noted. B The test article of the DMPC/cholesterol sodium cromoglycate liposomes containing no preservative, demonstrated slight treatment related effects, limited to slight conjunctival redness (Draize grade 1) which was noted in the treated eye of all animals at some point during the dosing period. In all cases this conjunctival redness cleared in less than 24 hours. The test article of the DMPC/cholesterol sodium cromoglycate liposomes containing preservatives BAK and PMA, demonstrated slight treatment related effects, limited to slight conjunctival redness (Draize grade 1) which was noted in the treated eye of three of the four test animals at some point during the dosing period. In all cases this conjunctival redness cleared in less then 24 hours. Other formulations of liposomal sodium cromoglycate are tabulated at Table 9. r ro TABLE2 CALCULATION OF TISSUE CONCENTRATIONS. VARIABLE AND CONSTANTS A = dpm IN TISSUE SAMPLE B = AVERAGE dpm IN SPIKED TISSUE SAMPLES C = AVERAGE dpm IN SPIKE D = AVERAGE dpm IN DOSE E = MASS(g) OR VOLUME(ml) tissue • 1mg = 1 x 10 6 ng=DOSE OF CROMOLYN CALCULATIONS FRACTION RADIOACTIVITY RECOVERED = F = B/C CORRECTED RADIOACTIVITY (dpm) = G = A/F % DOSE/g OR ml TISSUE = H = 100G/DE ng CROMOLYN/g OR ml TISSUE * = (1 x 10»)H/100 * ASSUMING ALL RADIOACTIVITY IS IN PARENT COMPOUND SUBSTITUTE SHEET TABLE3 COMPOSITION OF CHS-BASED CROMOLYN LIPOSOME TESTED IN BIODISTRIBUTION SCREEN. COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SORBIC ACID 0.05 DISODIUM EDTA 0.013 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM HYDROXIDE 12.3mEq/100ml(app) CHOLESTERYL ACID SUCCINATE 10.0 BUTYLATED HYDROXYTOLUENE 0.001 2.4 STERILE WATER FOR INJECTION, USP qs pH = 6.5-7.0 OSMOLALITY - 240 mmol/kg SUBSTITUTE SHEET TABLE4 FORMULA OF CHS-BASED CROMOLYN LIPOSOMES WITH SORBIC ACID AND METHYLPARABEN TESTED IN BIODISTRIBUTION SCREEN. COMPOSITION COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SORBIC ACID 0.2 METHYLPARABEN 0.1 DISODIUM EDTA 0.013 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM HYDROXIDE 12.3mEq/100ml(app) CHOLESTERYL ACID SUCCINATE 10.0 BUTYLATED HYDROXYTOLUENE 0.001 STERILE WATER FOR INJECTION, USP qs pH = 6.5-7.0 OSMOLAUTY » 240 mmol/kg SUBSTITUT E SHEET TABLE5 FORMULA OF DMPC/CHOL-BASED CROMOLYN LIPOSOMES WITH BENZALKONIUM CHLORIDE AND PHENETHYL ALCOHOL TESTED IN BIODISTRIBUTION SCREEN. COMPOSITION COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 BENZALKONIUM CHLORIDE 0.01 PHENETHYL ALCOHOL 0.4 DISODIUM EDTA 0.013 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.30 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10.0 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs pH = 6.5-7.0 OSMOLAUTY " 260 mmol/kg SUBSTITUTE SHEET TABLE 6 COMPOSITION OF HSPC/CHOL-BASED CROMOLYN LIPOSOME TESTED IN BIODISTRIBUTION SCREEN COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SORBIC ACID 0.05 DISODIUM EDTA 0.013 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.30 HYDROGENATED SOY PHOSPHATIDYLCHOLINE 10.0 CHOLESTEROL 2.4 BUTYLATED HYDROXYTOLUENE 0.001 STERILE WATER FOR INJECTION, USP qs pH - 6.5-7.0 OSMOLALITY = 240 mmol/kg SUBSTITUTE SHEET TABLE 7 DESCRIPTION CROMOLYN/DMPC/CHOLΛJNPRESERVED COMPONENT CONCENTRATION % (w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.5 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs ad SUBSTITUTE SHEET TABLE8 FORMULA FOR THE DMPC/CHOL-BASED CROMOLYN LIPOSOME WITH BENZALKONIUM CHLORIDE AND PHENYLMERCURIC ACETATE COMPONENT CONCENTRATION % (w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.42 DISODIUM EDETATE 0.013 BENZALKONIUM CHLORIDE 0.01 PHENYLMERCURIC ACETATE 0.004 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs ad SUBSTITUTE ShΞiT !_____> 1. DMPC/cholesterol without preservatives or EDTA. 2. DMPC/cholesterol with 0.01% benzalkonium chloride and 0.5% chlorbutanol. 3. DMPC/cholesterol with 0.01% benzalkonium chloride, 0.1% methylparaben, 0.1% propylparaben, and 0.5% chlorbutanol. 4. HSPC/cholesterol without preservatives or EDTA. 5. HSPC/cholesterol with 0.01% benzalkonium chloride and 0.5% chlorbutanol. 6. HSPC/cholesterol with 0.01% benzalkonium chloride, 0.1% methylparaben, 0.1% propylparaben, and 0.5% chlorbutanol. 7. DMPC/cholesterol with .004% phenylmercuric acetate, and 0.01%w/v benzalkonium chloride. SUBSTITUTESHEET TABLE 10 GRADING OF OCULAR LESIONS CORNEA OPACITY: DEGREE OF DENSITY (AREA MOST DENSE TAKEN FOR READING) NO ULCERATION OR OPACITY 0 SCATTERED OR DIFFUSE AREAS OF OPACITY (OTHER THAN SLIGHT DULLING OF NORMAL LUSTER), DETAILS OF IRIS CLEARLY VISIBLE 1 EASILY DISCERNIBLE TRANSLUCENT AREA, DETAILS OF IRIS SLIGHTLY OBSCURED 2 NACREOUS AREA, NO DETAILS OF IRIS VISIBLE, SIZE OF PUPIL BARELY DISCERNIBLE 3 OPAQUE CORNEA, IRIS NOT DISCERNIBLE THROUGH OPACITY 4 IRIS NORMAL 0 MARKEDLY DEEPENED RUGAE, CONGESTION, SWELLING, MODERATE CIRCUMCORNEAL HYPEREMIA OR INJECTION, ANY OF THESE OR COMBINATION THEREOF, IRIS STILL REACTING TO LIGHT (SLUGGISH REACTION IS POSITIVE) 1 NO REACTION TO LIGHT, HEMORRHAGE, GROSS DESTRUCTION (ANY OR ALL OF THESE) 2 CONJUNCTIVA REDNESS: (REFERS TO PALPEBRAL AND BULBAR CONJUNCTIVAE) BLOOD VESSELS NORMAL 0 SOME BLOOD VESSELS DEFINITELY HYPEREMIC (INJECTED) 1 DIFFUSE CRIMSON COLOR, INDIVIDUAL VESSELS NOT EASILY DISCERNIBLE 2 DIFFUSE, BEEFY RED 3 CHEMOSIS: LIDS AND/OR NICTITATING MEMBRANE NO SWELLING 0 ANY SWELLING ABOVE NORMAL (INCLUDES NICTITATING MEMBRANE) 1 OBVIOUS SWELLING WITH PARTIAL EVERSION OF LIDS 2 SWELLING WITH LIDS ABOUT HALF CLOSED 3 SWELLING WITH LIDS MORE THAN HALF-CLOSED 4 SUBSTITUTE SHEET TABLE 10 (CONT) DISCHARGE NO DISCHARGE 0 ANY AMOUNT DIFFERENT FROM NORMAL (DOES NOT INCLUDE SMALL AMOUNTS OBSERVED IN INNER CANTHUS OF NORMAL ANIMALS) 1 DISCHARGE WITH MOISTENING OF THE LIDS AND HAIRS JUST ADJACENT TO LIDS 2 DISCHARGE WITH MOISTENING OF THE LIDS AND HAIRS, AND CONSDERABLE AREA AROUND THE EYE 3 TEST SUBSTANCE CLASSIFICATION CATEGORY I CORROSIVE (IRREVERSIBLE DESTRUCTION OF OCULAR TISSUE) OR IRRITATION PERSISTING MORE THAN 4 DAYS CATEGORY II CORNEAL INVOLVEMENT AND/OR IRRITATION CLEARING IN 3-4 DAYS CATEGORY m CORNEAL INVOLVEMENT AND/OR IRRITATION CLEARING IN 2 DAYS OR LESS CATEGORY IV MINIMAL EFFECTS CLEARING IN LESS THAN 24 HOURS SUBSTITUTE SHEET TABLE 11 SUMMARY OF ANTIMICROBIAL EFFECTIVENESS TEST DATA FOR DMPC/CHOL-BASED CROMOLYN LIPOSOME SAMPLES (AL SAMPLES CONTAINED 0.013% EDTA) SUBSTITUTE SHEET TABLE 12 COMPOSITION OF DMPC/CHOL-BASED CROMOLYN LIPOSOMES: AME TESTING RESULTS CO c CD 3 H C H m co I rπ m TABLE 13 FORMULA FOR THE DMPC/CHOL-BASED CROMOLYN LIPOSOME WITH BENZALKONIUM CHLORIDE AND PHENYLMERCURIC ACETATE COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.42 DISODIUM EDETATE 0.013 BENZALKONIUM CHLORIDE 0.01 PHENYLMERCURIC ACETATE 0.004 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs ad SUBSTITUTE SHEET TABLE 14 FORMULA FOR THE DMPC/CHOL-BASED CROMOLYN LIPOSOME WITHOUT PRESERVATIVES DESCRIPTION CROMOLYN/DMPC/CHOLΛINPRESERVED COMPONENT CONCENTRATION %( /v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.5 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs ad SUBSTITUTE SHEET TABLE 15 FORMULA FOR THE DMPC/CHOL-BASED CROMOLYN LIPOSOME WITH BENZALKONIUM CHLORIDE AND CHLOROBUTANOL DESCRIPTION CROMOLYN/DMPC/CHOLVBAK/CLORBU COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.48 DISODIUM EDETATE 0.013 BENZALKONIUM CHLORIDE 0.01 CHLOROBUTANOL* 0.5 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs ad *A 25% MANUFACTURING EXCESS WAS USED TO COMPENSATE FOR AN ESTIMATED 25% LOSS DUE TO HIGH VOLATILITY SUBSTITUTE SH T TABLE 16 FORMUU FOR THE DMPC/CHOL-BASED CROMOLYN LIPOSOME WITH BEZALKONIUM CHLORIDE, CHLOROBUTANOL, METHYLPARABEN AND PROPYLPARABEN DESCRIPTION Cromolyn/DMPC/Chol/BAK/MPbn/Ppbn/Clorbu COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.46 DISODIUM EDETATE 0.013 BENZALKONIUM CHLORIDE 0.01 METHYLPARABEN 0.1 PROPYLPARABEN 0.1 CHLOROBUTANOL* 0.5 DIMYRISTOYLPHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 STERILE WATER FOR INJECTION, USP qs ad *A 25% MANUFACTURING EXCESS WAS USED TO COMPENSATE FOR AN ESTIMATED 25% LOSS DUE TO HIGH VOLATILITY SUBSTITUTE SHEET TABLE 17 FORMULA FOR THE HSPC/CHOL-BASED CROMOLYN LIPOSOME WITHOUT PRESERVATIVES DESCRIPTION CROMOLYN/HSPC/CHOL/UNPRESERVED COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.37 HYDROGENATED SOY PHOSPHATIDYLCHOLINE 10 CHOLESTEROL 2.4 BUTYLATED HYDROXYTOLUENE .001 STERILE WATER FOR INJECTION, USP qs ad SUBSTITUTE SHEET TABLE 18 FORMULA FOR THE HSPC/CHOL-BASED CROMOLYN LIPOSOME WITH BENZALKONIUM CHLORIDE AND CHLOROBUTANOL DESCRIPTION Cromolyn/HSPC/Chol/BAK/Clorbu COMPONENT CONCENTRATION %(w/v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.49 . DISODIUM EDETATE 0.013 BENZALKONIUM CHLORIDE 0.01 CHLOROBUTANOL * 0.5 HYDROGENATED SOY PHOSPHATIDYLCHOLINE 8 CHOLESTEROL 2.4 BUTYLATED HYDROXYTOLUENE .0008 STERILE WATER FOR INJECTION, USP qs ad *A 25% MANUFACTURING EXCESS WAS USED TO COMPENSATE FOR AN ESTIMATED 25% LOSS DUE TO HIGH VOLATILITY SUBSTITUTE SHEET TABLE 19 FORMULA FOR THE HSPC/CHOL-BASED CROMOLYN LIPOSOME WITH BENZALKONIUM CHLORIDE, CHLOROBUTANOL, METHYLPARABEN, AND PROPYLPARABEN DESCRIPTION Cromolyn/HSPC/Chol/BAK/MPbn/PPbn/Clorbu COMPONENT CONCENTRATION %(w v) DISODIUM CROMOGLYCATE 2.0 SODIUM PHOSPHATE, DIBASIC 0.71 SODIUM PHOSPHATE, MONOBASIC 0.46 DISODIUM EDETATE 0.013 BENZALKONIUM CHLORIDE 0.01 METHYLPARABEN 0.1 PROPYLPARABEN 0.1 CHLOROBUTANOL * 0.5 HYDROGENATED SOY PHOSPHATIDYLCHOLINE 8 CHOLESTEROL 1.92 BUTYLATED HYDROXYTOLUENE 0.0008 STERILE WATER FOR INJECTION, USP qs ad *A 25% MANUFACTURING EXCESS WAS USED TO COMPENSATE FOR AN ESTIMATED 25% LOSS DUE TO HIGH VOLATILITY SUBSTITUTE SHEET TABLE20 COMPOSITION OF CHS-BASED CROMOLYN LIPOSOMES SUBMITTED FOR ANTIMICROBIAL EFFECTIVENESS TESTING. CO c 03 CO H - . C H rπ fe CO _E rπ rπ H

Claims: CLAIMS 1. A liposome composition comprising cromolyn and lipid wherein the lipid is cholesterol hydrogen succinate. 2. The liposome composition of claim 1 additionally containing disodium EDTA and phosphate buffer to a pH around about pH 7.0. 3. The liposome composition of claim 2 additionally containing benzalkonium chloride and phenylethyl alcohol. 4. A liposome composition comprising a saturated phospholipid and cholesterol, cromolyn and phosphate buffer to a pH around about pH 7.0. 5. The liposome composition of claim 4 additionally comprising disodium EDTA. 6. The liposome composition of claim 4 wherein the phosphate buffer comprises sodium phosphate monobasic and sodium phosphate dibasic. 7. The liposome composition of claim 5 wherein the phosphate buffer comprises sodium phosphate monobasic and sodium phosphate dibasic. 8. The liposome composition of claim 4 wherein the ratio of cromolyn to lipid ranges from about 0.005 to about 20.0. 9. The liposome composition of claim 8 wherein the ratio of cromolyn to lipid ranges from about 0.071 to about 0.667. 10. The liposome composition of claim 9 wherein the ratio of cromolyn to lipid is about 0.2. 11. The liposome composition of claim 7 wherein the saturated lipid is dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), or hydrogenated soy phosphatidylcholine (HSPC). 12. The liposome composition of claim 6 wherein the saturated lipid is dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), or hydrogenated soy phosphatidylcholine (HSPC). 10 13. The liposome composition of claim 11 additionally comprising a preservative. 14. The liposome composition of claim 13 wherein the preservative is 5 phenylmercuric acetate. 15. The liposome composition of claim 13 wherein the preservative is benzalkonium chloride and chlorbutanol. 0 16. The liposome composition of claim 11 wherein the saturated lipid is dimyristoylphosphatidylcholine additionally comprising phenylmercuric acetate and benzalkonium chloride. 17. The liposome composition of claim 15 wherein the saturated lipid 5 is dimyristoylphosphatidylcholine or hydrogenated soy phosphatidylcholine, additionally comprising methylparaben and propylparaben. 18. The liposome composition of claim 5 wherein the saturated lipid 0 is dimyristoylphosphatidylcholine (DMPC), additionally comprising phenylmercuric acetate, and benzalkonium chloride or phenylethyl alcohol. g 19. The liposome composition of claim 5 wherein the saturated phospholipid is dimyristoylphosphatidylcholine (DMPC), additionally comprising benzalkonium chloride and thimersol. 20. A pharmaceutical composition comprising the liposome composition of claim 4 and a pharmaceutically acceptable carrier or diluent. 21. The liposome composition of claim 12 wherein the saturated lipid is hydrogenated soy phosphatidylcholine. 22. The liposome composition of claim 15 wherein the saturated lipid is hydrogenated soy phosphatidylcholine.

Inventors: BOLCSAK LOIS E, BOLCSAK, LOIS, E.
Applicants: LIPOSOME CO INC, THE LIPOSOME COMPANY, INC.
Application year: 1990
Main classification: A61K37
IPC codes: A61K9, A61K31, A61K47