Novel prostaglandin analogue

Abstract: The present invention provides a novel prostaglandin analogue of the following formula: and a method for its preparation.

Description: The present invention relates to a specific prostaglandin analogue. EP-A-0 073 397 generally discloses prostaglandin C-9 enol acylates. However, this reference does not disclose the specific prostaglandin analogue of the present invention. EP-A-0 150 732 discloses prostaglandin emulsion compositions. This reference does not disclose a prostaglandin analogue having a propylcarbonyloxy group attached in 9-position of a PGE, Structure. For prostaglandins (hereinafter referred to as PG), six structures i.e. PGE 1 , PGE 2 , PGE 3 , PGF 1α , PGF 2α and PGF 3α , were determined in 1960. Since then, PG analogues have been discovered one after another, and their physiological activities have been gradually known. For example, methyl 9-acetoxy-11α,15S-dihydroxyprosta-8,13E-diene-1-oate of the formula (A), methyl 9,11α,15S-triacetoxyprosta-8,13E-diene-1-oate of the formula (B), methyl 9,15R-diacetoxy-11α-hydroxyprosta-8,13E-diene-1-oate of the formula (C) and 9,15S-diacetoxy-11α-hydroxyprosta-8,13-E-diene-1-oate of the formula (D) may be mentioned (U.S. Patent 4,363,817). On the other hand, another literature predicts an important role which PGs will play as drugs in future and proposes that since PGs are typical local hormones which are produced locally as required and which act locally, it is necessary for such PG-related drugs to develop a drug delivery system taking into consideration the chemical properties and characteristics as autacoid. If a conventional general systemic administration method is employed, the effects are weak, and systemic side effects appear rather strongly. Therefore, it has been proposed to use lipid microspheres (hereinafter referred to as LM) as a carrier in the drug delivery system for PGs. However, such LM is believed to be emulsified fine particles of lipid containing PG. Namely, it has been reported that the stability in vivo is increased by formulating PGE 1 into an emulsion of lipid containing PGE 1 as a target treating drug having PGE 1 encapsulated in LM having a diameter of 0.2 µm, and the formulated drug shows a vasodilator activity and platelet aggregation inhibiting activity stronger than PGE 1 alone (Sim, A.K., et al, Arznein-Forsch/Drug Res., 1206-1209, 1986). Further, it has been reported that when the PGE 1 -containing lipid emulsion is administered to a vital body, a substantial amount of PGE 1 is freed from LM, and a study has been made to control the amount to be freed (Rie Igarashi et al, Ensho, 8 , (3), 243-246 (1988). In the report, it is reported that with respect to the methyl ester, ethyl ester, butyl ester, pivalic acid ester and octyl ester of PGE 1 , (1) the platelet aggregation inhibiting effect of each ester of PGE 1 was measured to study whether or not the activities would be obtained in vivo by the cleavage of the ester bond by an esterase even when the ester was per se inactive, and (2) the stability of each ester of PGE 1 as a LM drug was measured to study liberation of PGE 1 ester from LM by incubating it in a BSA-saline, to see the stability of the LM drug in blood (Tables 1(a) and 1(b) and Table 2). Liberation of PGE 1 and its esters from the respective LM drugs when incubated in a 1.6% BSA-saline solution % of PGE 1 (or ester) liberated from LM 1 min incubation (5%) 10 min incubation (%) PGE 1 96.2 ± 2.1 90.7 ± 2.4 PGE 1 methyl ester 82.5 ± 3.8 80.4 ± 2.9 PGE 1 ethyl ester 82.0 ± 8.8 125.4 ± 11.7 PGE 1 butyl ester 60.5 ± 4.0 65.3 ± 5.6 PGE 1 pivalyl ester 51.6 ± 2.8 83.1 ± 5.1 PGE 1 octyl ester nd nd In order to improve the gradual releasability of PGE 1 esters, it is necessary to finely disperse PGE 1 ester-containing LM in water for the production of LM drugs. For this purpose, PGE 1 esters, lipids and other materials are required to be homogenized in water at a high temperature of from about 80 to 90°C, as will be described hereinafter. Under such a high temperature condition, conventional PGE 1 is likely to be rapidly decomposed. Further, conventional PGE 1 is poor in the storage stability and is likely to undergo decomposition also in the course of distribution of the commercial products. It is a first object of the present invention to develop PGE 1 analogues excellent in the stability even when formulated under a high temperature condition. It is a second object of the present invention to provide a process for preparing a PGE 1 analogue excellent in the stability even when formulated under high temperature condition. Namely, the present invention provides a prostaglandin analogue of the following formula: namely butyl 9-butyroxy-11α,15S-dihydroxyprosta-8,13E-diene-1-oate. The present invention also provides a process for preparing the prostaglandin analogue of claim 1, characterized in that (1E,3S)-1-iodo-3-(t-butyldimethylsiloxy)-1-octene is reacted with an alkyl lithium to obtain the corresponding 1-lithio octene which is then reacted with a tri-alkylphosphine-copper(I) iodide complex to obtain the corresponding organolithiocuprate, which is then reacted with 4R-t-butyldimethylsiloxy-2-(6-carbobutoxyhexyl)-2-cyclopentene-1-one and then the reaction mixture is quenched with butyric anhydride. Now, the present invention will be described in detail. The PG analogue of the present invention can be produced by a known process. For example, it may be produced by a process which comprises reacting a substituted 1-iodoalkene with an alkyl lithium to obtain a substituted 1-lithioalkene, then reacting it with a trialkylphosphine-copper (I) iodide complex to obtain an organolithiocuprate, and then reacting this organolithiocuprate with a substituted 2-cyclopenten-1-one by 1,4-conjugation addition, and then quenching the reaction mixture with a carboxylic anhydride, carboxylic mixed anhydrides or a carboxylic acid halide. The details of this process are disclosed in e.g. U.S. Patent 4,363, 817 or in literatures by Sih, et al. J. Am. Chem. Soc., 97, 857,865 (1975), J. Am. Chem. Soc., 110 , 3588 (1988). Now, the present invention will be described in further detail with reference to the Example and Reference Example. Reference EXAMPLE Preparation of butyl 9-actoxy-11α,15S-dihyroxy-17S,20-dimethylprosta-8-13E-diene-1-oate A solution of (1E,3S,5S)-1-iodo-3-(t-butyl dimethylsiloxy)-5-methyl-1-nonene (5.38 g, 13.56 mmol) in ethyl ether (100 mℓ) was cooled to -78°C, and t-butyl lithium (f = 1.5 hexane solution 18.1 mℓ, 27.1 mmol) was dropwise added thereto. The mixture was stirred at the same temperature for 2 hours, and then a solution of a tributylphosphine-copper(I) iodide complex (4.63 g, 12.31 mmol) and tributylphosphine (2.92 mℓ, 12.16 mmol) in ethyl ether (40 mℓ) was dropwise added thereto. The mixture was stirred at -78°C for 50 minutes, and then a solution of 4R-t-butyldimethylsiloxy-2-(6-carbobutoxyhexyl)-2-cyclopenten-1-one (4.75 mℓ, 11.3 mmol) in ethyl ether (160 mℓ) was dropwise added thereto. The mixture was stirred at -78°C for 20 minutes and further at from -23 to -18°C for 35 minutes, and then acetic anhydride (3.0 mℓ, 30 mmol) was dropwise added thereto at 0°C, followed by stirring at from 0°C to room temperature for 15 hours. Then, a saturated ammonium sulfate aqueous solution (200 mℓ) was added thereto. After separating from the organic layer, the aqueous layer was extracted twice with ethyl ether (100 mℓ), and the extract was combined to the organic layer. The combined organic layer was washed with a saturated sodium chloride aqueous solution (120 mℓ). The organic layer was dried over anhydrous magnesium and then filtered. The solvent was distilled off under reduced pressure. The residue was purified by silica gel chromatography (hexane/ethyl acetate = 20/1 to 4/1) at 0°C to obtain an adduct. The adduct thus obtained (5.5 g, 8.25 mmol) was dissolved in acetonitrile (100 mℓ), and a 40% hydrofluoric acid aqueous solution (10 mℓ) was added thereto at 0°C. The mixture was stirred at the same temperature for 30 minutes. The reaction solution was poured into a mixture of a 20% potassium carbonate aqueous solution (150 mℓ) and methylene chloride (150 mℓ). The mixture was dried over anhydrous magnesium sulfate and then filtered, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel chromatography (methylene chloride/acetone = 2/1) at 0°C to obtain the above identified compound (3.27 g, yield: 83%). 1 H-NMR(CDCℓ 3 ): δ 0.8-1.0(9H,m), 1.2-2.9 (30H,m+s(δ2,15,3H)), 3.1(1H,m), 4.05(2H,t,J=7Hz), 4.1-4.2(2H,m), 5.48(1H,dd,J=7Hz), 5.6(1H,dd,J=7Hz) EXAMPLE Preparation of butyl 9-butyroxy-11α,15S-dihydroxyprosta-8,13E-diene-1-oate The above identified compound was obtained in a yield of 73.6% in the same manner as in the Reference Example except that (1E,3S)-1-iodo-3-(t-butyldimethylsiloxy)-1-octene was used instead of (1E,3S,5S)-1-iodo-3-(t-butyldimethylsiloxy)-5-methyl-1-nonene as the ω chain, and butyric anhydride was used instead of acetic anhydride. 1 H-NMR(CDCℓ 3 ): δ 0.86-1.0(9H,m), 1.2-2.42(29H,m), 2.8-2.95(1H,m), 3.0-3.1(1H,m), 4.0-4.2(4H,m), 5.4-5.7(2H,m) The stability during the preparation and the remaining rate in the storage stability was measured by a separation quantitative analysis by means of high performance liquid chromatography, and the ratio of the measured amount relative to the amount at the initiation of the test was taken as the remaining rate. As compared with the emulsion of PGE 1 , the emulsion of the PG analogue of the present invention was superior in the stability during the preparation and in the stability during the storage. In addition to such effects, the emulsions of the PG analogue of the present invention are expected to be effective in the gradual releasing properties, focus selectivity, quick-acting properties and reduction of side effects.

Claims: A prostaglandin analogue of the following formula: namely butyl 9-butyryloxy-11α,15S-dihydroxyprosta-8,13E-diene-1-oate. A process for preparing the prostaglandin analogue of claim 1, characterized in that (1E,3S)-1-iodo-3-(t-butyldimethylsiloxy)-1-octene is reacted with an alkyl lithium to obtain the corresponding 1-lithio octene which is then reacted with a tri-alkylphosphine-copper(I) iodide complex to obtain the corresponding organolithiocuprate, whcih is then reacted with 4R-t-butyldimethylsiloxy-2-(6-carbobutoxyhexyl)-2-cyclopentene-1-one and then the reaction mixture is quenched with butyric anhydride.

Inventors: INOMATA TOSHIHIDE, MIZUSHIMA YUTAKA, YASUDA ARATA, INOMATA, TOSHIHIDE, MIZUSHIMA, YUTAKA, YASUDA, ARATA, INOMATA, TOSHIHIDE, C/O SEIKAGAKU KOGYO CO., LTD.
Applicants: ASAHI GLASS CO LTD, MIZUSHIMA YUTAKA, SEIKAGAKU KOGYO CO LTD, YOSHITOMI PHARMACEUTICAL, ASAHI GLASS COMPANY LTD., MIZUSHIMA, YUTAKA, SEIKAGAKU CORPORATION, YOSHITOMI PHARMACEUTICAL INDUSTRIES, LTD.
Application year: 1990
Main classification: C07C405
IPC codes: C07C69, C07C405, A61P43, A61K31, A61K9