USE OF HONEY AS VACCINE

Abstract: Anti-honey food products are disclosed, such food products being obtained from animals hyperimmunized against at least one antigen found in honey. A vaccine producing said anti-honey food product is disclosed, said vaccine comprising at least one honey antigen. Further, a dietary supplement for humans and especially infants which protects against harmful allergens and microorganisms found in honey is disclosed, said supplement comprising the anti-honey eggs or milk or fractions thereof.

Description: TTHE OF THE INVE TTCN USE OF HCNEY AS VACCINE C- ** OSS-REEΕKE CES TO EEIAJED DOCUMENTS The present application is a continuation-in-part of U.S. 4,879,110, filed February 26, 1988 which is a contiπuation-in- part of Serial No. 001,848, filed. January 9, 1987, which is a division of U.S. Patent No. 4,636,384, which is a coπtiπuation-in-part of Application Serial No. 384,625, filed June 3, 1982, new abandoned. Ba.CK5_RO0ND OF THE INVENTION FIELD OF THE INVENTION This invention relates to the use of honey and/or bee as a cαπponent of a vaccine for iiTtmunizing cows and/or chickens against the allergens and it croorganiππis contained therein. The invention further relates to food products such as rαilk and/or eggs from animals hyperdαπmunized against honey and/or the bee, and to the use of such food products, as a source of anti-honey factors which protect a host which ingests such food products against the allergens and ιricroo__g__-isms found in honey. DESC- ** ___ * TTON OF THE BACKGROUND ART The body of the bee, more specifically the hind legs, is used to collect pollen that is used as a food by the bees in the hive. Many of the pollens are allergens. These are the same allergens (for example, ragweed) that cause e__vironment- related allergic reactions in humans or other animal species living in the area. As a result, honey made by the bee, and the body of the bee, carry a randctα sample of various pollens and other allergens or .microorganisms that are indigenous to a specific geographical area. Bees and honey from different geogr__phical areas carry different kinds of pollen because the vegetation varies. The allergic properties of honey have been described. (Miedzcbrodza, A. et al.. Przecrl. JDsk. 34:721-724 (1977); Bonneau, J.C. et al.. Allem. -Iiππiuriol. 17:526-527 (1985)). In susceptible individuals honey can induce bronchial asthma attacks, and allergic and antitxx -mediated hypersensitivity reactions, such attadks may be severe and be accompanied by tissue damage. Young infants are unusually susceptible to toxic πac-τ_organisms in honey. For a food product, honey provides an unusually cxDncentrated source of spores of Clostridium botulinum, the microorganism responsible for botulism. Clostridium botulinum has been estimated to contaminate 10-15% of all honey. (Brown, L. ., J. Pediatr. 94:337-338 (1979); Sp ka, J.S. et al.. Am. J. Pis. Child. 143:828-832 (1989)). " Infortunately, honey is often used as an alternative to cane sugar in infant formula preparations and infant botulism is now the most common form of botulism identified in this country. Honey is the only food source thus far implicated in the pathogenesis of infant botulism. Approximately 15% of the infant botulism cases identified in the united States are associated with ingestion of honey (Spika, J.S. et al.. Am. J. Pis. Child. 143:828-832 (1989); Hausciiild, A.H. . et al. J. Food Prot. 51:892-894 (1988)). Infant botulism may also arise due to ingestion or inhalation of the C. botulinum spores from eπv_bπ_a_mental exposure to dust or dirt. When ingested, toxin is formed within the gut of the infant after the germination and multiplication of the C. botulinum spores. Release of botulism toxin in adults and especially in infants and children can be fatal. Treatment of botulism in infants is merely supportive. Thus, it would be advantageous to have a method for the treatment or prevention of infant botulism. It has been known in the prior art to produce milk having a variety of therapeutic effects. Beck, for example, has disclosed a milk containing antribody to Støphylococcus mutans that has dental caries-inhibiting effects (Beck, U.S.. Patent 4,324,782; British Patent 1,505,513). The milk is obtained by hyperiiiimunization of a cow with St_reptocoσcus mutans antigen and collecting the therapeutic milk therefrom. Beck has also described a milk having anti-arthritic properties (U.S. Patent 4,732,757) , and has patented a method of treating inflammation using milk from hyperimraunized cows (Beck, U.S. Patent 4,284,623). Stolle has disclosed a method of using milk from a hyperimmunized ccw to treat diseases of the vascular and pulmonary systems (Stolle et al.. U.S. Patent 4,636,384). Heihbach, U.S. Patent 3,128,230, has disclosed milk containing alpha, beta and gamma globulins against anti-9-enic haptens. Sir (U.S. Patent 3,911,108), Peterson (U.S. Patent 3,376,198 and Canadian Patent 587,849), Holm (U.S. Application (publish¬ ed) Serial No. 628,987), Tannah et al. (British Patent 1,211,876), and Biokema S.A. (British Patent 1,442,283) have also described antiboά -∞nt-aiiiing milks. In U.S. Patent 4,636,384, and U.S. Application No. 001,848, filed January 9, 1987, there was disclosed a method of lowering blood lipid levels and treating lipid-associated vascular disorders, as well as treating inacrcphage-related pulmonary disorders, cαiprising feeding test animals and humans antibody-containing milk derived from σcws maintained in a hyperim une state by injections of polyvalent antigens derived frcarα mixtures of killed bacteria. In another expend¬ ing application (U.S. Application No. 069,139, filed July 2, 1987) , there was disclosed use of anti__ody-<x>ntaϊni_ng milk derived from hyper_i_mmunized ccws for the treatment of gastro¬ intestinal disorders. It is well known to those skilled in the art of immunol¬ ogy that serum globulin fractions consisting of various antibody types such as, for example, IgA, IgG, and Ig , can be used to counter corresponding antigens, thereby neut_ralizing the effects of the antigens. There are an almost infinite number of harmful antigens to which animals can be exposed, including carcinogenic, bacterial, viral, and regulatory factors of plant and animal origin, as well as toxins and poisons. Normally, upon exposure to a foreign antigen, e.g., pollen, the immune system of the host will produce antibodies that will iveutralize the effects of the antigen. Exposure to such foreign antigens can occur either naturally, or deliberately by administration of the antigen in vaccine form. The latter is generally referred to as active immunization of the host species exposed to the antigen. The antibodies produced in response to such vaccination are homologous to said given species of animal, and are epitopic to the antigen. It is known that various genera of the class Ayes, such as chickens (Gallus dσmesticus) , turkeys, and ducks, produce antibodies in their blood and in their eggs against factors which cause avian diseases, as well as against other antigens. For example, LeBacxj-Verheyden et al.. Immunoloqy 27:683 (1974), and Nestle, G.A. et al. ' . J. Mad. 130:1337 (1969), have quantitatively analyzed immι_rκ___)glc_-ulins of the e cken. Poison, A. et al.. In-nunolocfical O-mrniunications 9:495-514 (1980) immunized hens against several proteins and natural mixtures of proteins, and detected IgY antibodies in the yolks of the eggs. Fertel, R. et al.. Biochemical and Biophysical Research Commurαcations 102:1028-1033 (1981) immunized hens against prostaglaridins and detected antibodies in the egg yolk. Jensenius et al., Journal of Immunolo-τical ethods 46:363-368 (1981), provide a method of isolating egg yolk IgG for use in immunodiagnostics. Poison et al.. Tmrπunolocfical Qc-πmunications 9:475-493 (1980), describe antibodies isolated from the yolk of hens that were immunized with a variety of plant viruses. Poison, U.S. Patent 4,357,272, discloses the isolation of antibodies from the yolks of eggs derived from hyperij-ππunized hens. The hyperiαTirπunization was elicited by repetitive injections into the hens of antigens represented by plant viruses, human IgG, tetanus antitoxin, snake antivenins, and Serameba antigens. Poison, U.S. Patent 4,550,019, discloses the isolation from egg yolks of antibodies raised in the hen by hyperijrπrπunization with immunogens having a molecular or particle weight of at least 30,000. The antigens used to hyperimmunize the chickens were selected from among plant viruses, human ύximurioglobulins, tetanus toxin, and snake venoms. In Serial No. 577,804, there is disclosed a method of passive immunization of a mammal which comprises parenterally injecting a purified heterologσus antibody obtained from the eggs of a domesticated fowl, which species has been i_mmunized against an antigenic substance, and wherein the mammal has a history of consumption of eggs from such domesticated fowl. The invention disclosed in U.S. Serial No. 622,130 ejφands on the concepts disclosed in U.S. Serial No. 577,804, in that adπάiiistration of the egg antibody can be by any appropriate route, not only pa_renteral. The present invention is a further development over the inventions disclosed and claimed in the aforementioned applications, the entire disclosures of which are herein ■ incorporated by reference. SCMMftKY OF THE INVEWEION It is an object of the invention to produce food products, and specifically avian eggs and bovine milk which, when consumed by humans and other warm-blooded animals, will protect such animals against the damage caused by allergens, toxic microorganisms and other antigens found in honey. It is also an object of the invention to provide a method of treating or protecting human infants against the effects of ingested spores of C. botulinum. The invention provides materials and a process for producing such food products, and especially such avian eggs and bovine milk, such materials comprising honey and/or bee antigens in vaccine form, and such process ccmprising imraurazation and hyperiimπunization of avians and bσvines with said antigen vaccine. These and other objects of the invention, which will hereinafter become more readily apparent, have been obtained by providing honey and or bee as a vaccine for .ra urάzing bσvines and chickens. The methods of the invention craπprise administe_ring to an animal which is susceptible to allergens, antigens or toxins found in honey, egg products collected from avian genera maintained in a hype_ri_-_πune state against honey antigens and/or milk products from bovine genera maintained in hyperimmune state against honey antigens. Upon consumption by the animal being treated, the food products of the invention protect against tissue damage induced by the inhalation or σonsuitption of honey allergens, antigens or toxins. EEP TT-Rn πi ** -^CR__I : TTON OF THE JA& _?_KKb_t) IMBC»IME_N1S As used hereinafter, the terms "anti-honey allergen egg(s)" or "anti-honey allergen milk" refer to the avian eggs or bovine milk respectively, of the present invention, said eggs or milk produced by maintaining the avian or bovine animal in a hypeαrimmune state against the specific class of antigenic materials found in honey or on the outer surface of the bee's coat or legs. The term "honey antigen(s)", as used hereinafter, shall mean those antigens derived from bee honey and or the honeybee. Especially, the term "honey antigens" refers to pollens and ii croorganisms which can be extracted from honey or which are carried by the bee to the hive on its coat or legs. As used hereinafter the term "vaccine" refers to a suspension of honey and or honeybee or seme antigenic pollen-containing fraction thereof which, when administered to an avian or bovine, results in the production of antibodies against said antigen. (Pavis, B.D. et al.. Microbioloqy, 3rd ed., 1980, at p. 294) . The invention cαπprises a natural food product (anti- honey eggs and or anti-honey milk) which has beneficial .uπmunological and tissue protection properties towards animals which ingest it, as well as a method for producing and using the same. The anti-honey eggs and milk of the present invention, being natural products, can be used to prevent the allergic reactions and toxic responses associated with inhalation or ingestion of honey allergens, antigens or toxins without fear of side effects. In the process of this invention, the source animal includes any πύlk-producing animal of the genus Bos, preferably but not limited to the domesticated cow, and any egg-producing member of the class Aves. preferably, but not limited to, domesticated chickens (genus Gallus domesticus) . Accordingly, the genera represented by cxws and the like may be used as the source of the hypeαr-uπmune milk, and genera represented by turkeys, ducks, geese, and the like may be used as the source of the hyperimrπune eggs. The invention is based on the discovery that when such avians or bσvines are brought to a specific state of immunization by means of periodic booster ad_mini_≤trations of the specific class(es) of antigen contained in honey, or found on the body of the bee, or a mixture of such antigens, the animal will produce food products such as eggs or milk which, when consumed, have beneficial properties in the treatment of allergic, immunological and toxic effects of exposure to such antigens, whether such exposure occurs due to inhalation of the antigen from the environment, or to ingestion of the antigen in a food source. The food products of the invention which contain these beneficial properties include eggs and milk from animals hypearimmunized with such honey an_/or bee antigens and are herein called "anti-honey" eggs and "anti- honey milk." The beneficial ήπraunological and toxicological properties of the food products of the invention are called herein "anti- honey" properties and are not produced by all avians or bσvines that are simply immunized. That is to say, the induction of immune sensitivity alone is insufficient to cause the appearance of the aforementioned anti-honey properties in eggs and milk, as is shewn by the fact that normal fowl eggs or bovine milk do not contain these properties, even though fowl and bσvines have become sensitized against various antigens during normal imπamizatiαn against fcwl and bσvines diseases. Furthermore, the properties are not always present in eggs or milk produced by fcwl or bσvines maintained in the immune state by booster injection. It is only in a specific hyperimmune state that the eggs or milk produced have the desired effect. This special state is achieved only by administering periodic boosters with sufficiently high doses of specific honey antigens or mi_xtures of such antigens. The preferred dose range should be equal to or greater than 50% of the dosage necessary to cause primary sensitization of the avian or bovine. Having kncwledge of the -requirement fo developing and maintaining a hyperimmune state, it is within the skill of the art to vary the amount of honey antigen administered, depending upon the avian or bovine genera and strain employed, in order to maintain the animal in the hyperimmune state. In summary, the process ccπprises the following steps: 1. Selection of the honey and/or bee as a source of antigens. 2. Sensitization of avians or bσvines by primary immunization. 3. Adπ nistering boosters of honey and/or bee antigens of appropriate dosage to induce and maintain a hyperimmune state. 4. Collecting eggs or milk from the animal during the hyperimmune state. 5. Testing anti-honey properties of eggs or milk collected from said hyperimmune avian or bovine. Step 1—Selection of the honey and or bee as a source of antigens. The avian or bovine is immunized and hyperimmunized with a specific anti-honey vaccine which contains the honey and/or bee antigens. In a preferred eiibodiment whole honey is used in an iinfractionated form. The high viscosity of the honey, due to the high cx_ncentratica of sugar, may aid the immunization process by acting as an adjuvant for the antigens contained therein. In another enibodiinent bees, or the coats or legs thereof, or extracts containing wεushings £rcm the coats or legs of bees, are used in the vaccine. The avian responds to administration of this vaccine by producing antibodies in the eggs, against the iramunogens in the honey or from the bee used for the immunization; the bovine responds to administration of this vaccine by producing antibodies in the milk against the immunogens in the honey or from the bee used for the immuiizaticin. .Drimunological factors such as specific egg antibodies or specific milk antibodies, produced in response to the immunization, result in the anti-honey factors which provide the beneficial properties to the food products of the invention. Step 2—Honey or bee antigens can be administered by any method which causes sensitization. The preferred method of immunization is by _i_ntramuscular injection. The preferred method of administration of the antigens to chickens is in the breast muscle. The dosage is preferably 1-5 mg of the honey or bee vaccine. Repeated immunizations are given at intervals, preferably two-week, over a suitable period of time, preferably six months. It can be determined whether or not the animal has become sensitive to the antigen. There are a number of methods known to those of skill in the art of immunology to test for sensitivity. Methods in .Dπmunoloσv and Imπurκ3chemistry. Williams, CA., Chase, W.N., Academic Press, N.Y., London (Vols. 1-5) (1977) . The appearance of antibodies in the egg or milk after i__mmunization with the vaccine is indicative of sensitivity. The minimum dose of antigen necessary to induce hypersensitivity depends on the type of antigen used. Step 3 involves the induction and maintenance of the hyperimmune state. This state is inducted by repeated booster administration of an appropriate dosage at fixed-time intervals, preferably two-week intervals over a six-month period of time where honey or bee antigens are employed. Moreover, the booster adπ nistration must not induce a state of immune tolerance. This will cause the animal to pass from a hyperimmune state to a state of immune tolerance to the antigen, in which case the animal will cease to produce foor products such as milk or eggs with the beneficial properties. It might, also be possible, for example, to use a ccmbination of different ___romur_tization prcxsdiires, i.e., intramuscular injection for primary immunization and intra¬ venous injection for booster injections, etc. Many different combinations of immunization might be employed by those skilled in the arts to: (1) sensitize and (2) induce the hyperimmune state. Step 4 involves collection and processing of the eggs or milk. If the eggs or milk are to be processed into dried egg or dried milk pcwders, freeze-drying (lyophilization) is the preferred method. Whole eggs or milk can also be used, as well as eggs that have been separated into egg yolks and egg white and skim milk. The beneficial factors, including the beneficial antibodies are present in the egg yolk. Step 5 is to test the ability of the anti-honey food products to prevent tissue damage caused by antigen-induced allergic responses. Such properties can be deaionstrated by stuctying the effect of feed g the milk or eggs of the invention to animal models exposed to antigens viiich induce such tissue damage. The c πmon white laboratory rat is the preferred model. laboratory rats keep under controlled laboratory conditions and fed normal rat food can easily be exposed to antigens which promote the type of tissue damage prevented by ingestion of the food products of the invention. The preferred method of exposure to induce such tissue damage is to administer the antigen in an aerosol form. For example, one model system which may be used is to expose the rat to smoke which contains such antigens. Inhaled smoke which contains damaging antigens results in damage to the lung tissue which is a direct result of the allergic response to the antigens in the smoke. Such tissue damage to the lungs can be revealed by histological examination of the lungs. The protective properties of the food products of the invention is tested by feeding the food product, for example, the anti-honey milk arx_/or anti-honey egg, to the rats that are exposed to antigen-containing smoke in this manner. The inventor has discovered that smoke produced by the ccaiibustion of tobacco products, i.e. ' cigarette smoke, contains antigens which induce an allergic reaction in rat lung which is prevented by the food products of the invention vfaen such food products contain the above-described anti-honey protective factors. The food products of the invention can be provided in any amount which, in warm blooded animals, effects (brings about or induces) the reversal of tissue damage or toxicological interactions caused by honey antigens and _mica-corganisms. Such effect may also consist of a prevention of the occurrence of such damage in the first place, or of maintaining such a preventive effect. An animal being administered the food products of the invention in a manner which provides efficaciσus amounts of the anti-honey protective factors so as to effect a reversal of such tissue damage, or prevent an occurrence of such tissue damage or maintain a preventive effect against the ocx_urrenαe of such tissue damage, is said to be being "treated" according to the methods of the invention. The same amounts can be utilized in subjects vAien the food products of the invention are ingested so as to provide a preventive effect against subsequent exposure to antigens contained in honey or carried on the bee. The food products of the invention, such as whole eggs or whole milk or egg yolks or skim milk, can be incorporated into any food product, as long as the food product is not treated at a teπperature which is too elevated and which would thereby inactivate the beneficial anti-honey properties of the product. Further, it has been found that the yolk fractions of eggs contain the agent or agents responsible for the beneficial properties observed and referred to above. Those of ordinary skill in the art, }rιcwing that the yolk fraction contains the factors of inportanσe, would clearly recognize that further separation can be made to obtain more potent fractions. The preferred dose of the anti-honey milk is 50 grams of milk powder reconstituted in 8 oz. of water taken once daily. The effective dose may very among individuals from approximately 25 to 100 grams. One quart of fresh whole anti-honey milk contain approximately 90 grams of solids. The milk may be converted into a powder by any technique ccmmonly employed in the art, provided that the process does not involve heating the milk to such a teπperature that would destroy the beneficial anti-honey properties. The preferred dose of the anti-honey egg is the antibody equivalent of one whole egg yolk taken once daily. The effective dose may vary among individuals from 1/4 of an egg to 3 eggs. The antibody content of one whole egg is approximately 15 milligrams. The food products of the invention, aπti-hαney avian egg and anti-honey bovine milk may be ii-corporated into infant formula as infant food, so as to provide to infants who ingest this formula, and especially to infants less than two months old, the beneficial anti-honey properties and especially those anti-honey properties which confer protection against the ingested spores of Clostridium botulinum. An advantage of such administration is that the protective factors are administered to the infant or child in a food product such as milk which is usually tolerated by the infant or child. It is also an advantage that by ingesting the protective factors, such protective factors are administered directly into the intestinal tract of the infant or child. Having now generally described this invention, the same will be further described by reference to certain specific examples which are provided herein for purposes of illustra¬ tion only and are not intended to be limiting unless otherwise specified. EXftMPIE 1 IMMUNTZATTON AGAINST HONEY ANTIGENS Bee honey contains a diverse group of antigens, the exact composition of which is unknown. The antigen in honey is capable of inducir_g a hype_rimmune state when injected in cxws. Ccws were immunized once a week, intramuscularly, for three weeks, then at two week intervals for an additional two to three months. A single dose consists of 2.5 ml sterile distilled water and 2.5 ml raw honey. Milk was collected for the two weeks following the final injection. In another example a dose of 1 ml of honey diluted 1:5 in water was injected into the chiciken breast muscle, one a week for five consecutive weeks, then once every two weeks for six months. Eggs collected between the third and sixth months and tested for antibody were able to induce and maintain the hyperimmune state in the animal. Bσvines and avians may also be immunized with homogenized bee, or with washings of the coats of bees, in a similar manner. EXftMPIE 2 ANTT-HONEY OR ANTI-BEE MHK PROTECTS DUNG TISSUE FROM DAMAGE INDUCED BY ANTIGENS IN CIGARETTE SMOKE Forty-one adult f male Charles Rivers rats were used for this study. All rats received Wayne Rodent laboratory Chew ad libitum. Three times daily they were exposed to cigarette smoke (30 miriutes/exposure) . The experiment lasted three months. Rats were divided into five groups. Group I (7 rats) received water to drink. Group II (7 rats) received regular skim milk which had been pasteurized under conditions of low t__mperature (U.S. 4,879,110), that is, under conditions which did not raise the pasteurization te_rrpe_cature so high as to inactivate the beneficial components of the milk of the invention. Such pasteurization is herein called special pasteurization. Group III (9 rats) drank conventionally pasteurized anti-honey skim milk. Conventionally pasteurized milk is heated at a teitperature which de_-troys the beneficial cσnponents of the milk of the invention. Group IV (9 rats) received specially pasteurized anti-honey skim milk. Group V (9 rats) received specially pasteurized antirbee skim milk. Water for rats in Group I, and the abσve-described milk products for rats in Groups H through V, was available ad libitum. Liquids were changed twice daily. The animals drank from sterilized bottles fitted with stainless steel drinking tubes. After three months, all rats were sacrificed. Each rat was injec ed into the peritoneal cavity with an overdose of neαributal. Thoracic.and cervical viscera were exposed, and a blood sample was taken from the heart. A hemostat was clamped σver the trachea at the inferior border of the larynx. A 23 gauge hypodermic needle was inserted into the trachea and a fixative of 1% glutaraldehyde and 1% paraformaldehyde (pH 7.3) was infused into the tracheal lumen. This method of fixation was used in an attempt .to preserve the trachea and lungs as close to the "living state" as possible. After the pulmonary tissue had hardened, it was processed further by standard, techniques for viewing in the scanning electron microscope. Particular attention was given to the tracheal epithelium and to the ijitrapul onary bronchi and other air tubes as far as the respiratory bronchioles. With the scanning electron microscope, specimens could be viewed at magnifications ranging fro 10X to 50,000X. It was readily apparent that the cigarette smoke caused severe metaplasia to the lining epithelium in the tracheas of rats in Group I (water, Group II (specially pasteurized regular skim milk) , and Group III (conventionally pasteurized anti-honey skim milk) . As much as 100% of the tracheal epithelium in these rats was damaged. In contrast, the tracheal epithelium of rats in Group IV (specially pasteurized anti-bee milk) manifested a very high degree of protection. Even under the severe conditions of smoke inhalation used in these studies, there was little damage to the tracheal epithelium in most rats of Groups IV and V, and no damage at all to the tracheal epithelium in several of the rats. It was concli-ded that the tracheal epithelium in rats of Groups IV and V was protected by sane special σcπponent in the milk that the rats drank. This substance was not present in regular milk that was specially pasteurized (rats in Group II), or in conventionally pasteurized anti-honey milk (rats in Group III) , and it was not present in the water or solid diet consumed by the rats (rats in Group I) . The cigarette smoke also affected the epithelium of the bronchi and intrapulmonary air passages through the respiratory bronchioles in much the same manner as it did the tracheal epithelium. Rats in Group IV and V received protection, and rats in Groups I, II, and III did not receive protection. Now having fully described this invention, it will be understood by those with skill in the art that the scope may be performed within a wide and equivalent range of conditions, parameters, and the like, without affecting the spirit or sccpe of the invention or any eπbodiment thereof.

Claims: WHAT IS NEW AND INTENDED TO BE COVERED BY UtflTEKS PATENT OF THE UNITED STATES IS: 1. Anti-honey avian egg. 2. Anti-honey bovine milk. 3. A food product cαrprising said egg of claim 1 or said milk of claim 2. 4. A vaccine for the preparation of an anti-honey food product comprising at least one honey antigen. 5. A method for producing an anti-honey egg comprising: immunizing a female avian with honey; ma_mtaining said female avian in a hyperimmune state by adπlnistering to said female avian booster injections of said honey; and collecting eggs from said avian in a hyperiimπune state. 6. The method of claim 4 wherein said female avian is a female chicken. 7. A method for producing anti-honey milk comprising: imπunizing a cow with honey; irainta ing said cow in a hyper_j_π_πune state by administering to said cow booster injections of said honey; and collecting milk from said cow in a hyperimmune state. 8. A method of preventing tissue injury which occurs in response to allergic or toxic reactions induced by honey allergens in a warm-blooded animal which comprises administering to said animal an anti-honey food product, in an amount and for a time sufficient to produce an anti-allergic or anti-toxic effect. 9. The method of claim 8, wherein said food product is selected from the group consisting of anti-honey avian egg, anti-honey avian egg yolk, anti-honey bovine milk and anti- honey bovine skim milk. 10. The method of claim 8, wherein said food product is in powdered form. 11. The method of claim 8, wherein said food product is in liquid form. 12. The method of claim 8, wherein said warm-blooded animal comprises humans. 13. The method of claim 12, wherein said human is an infant less than two months old. 14. The method of claim 13, wherein said anti-allergic or anti-toxic reaction confers protection against Clostridium botulinum.

Inventors: STOLLE RALPH J, STOLLE, RALPH, J.
Applicants: STOLLE RES & DEV, STOLLE RESEARCH & DEVELOPMENT CORPORATION
Application year: 1990
Main classification: C07K15
IPC codes: A23C9, A23K1, A23L1, A61K35, A61K39, A61P37, C07K16