Anticancer agent

Abstract: An anticancer agent containing a pharmacologically effective amount of compound of formula Y-(X) m -Z, wherein Y is hydrogen or a polypeptide corresponding or functionally equivalent to the polypeptide of the cell-binding domain of the fibronectin molecule, X is a spacer, m is 0 or 1, and Z if -OH or a polypeptide corresponding or functionally equivalent to the polypeptide of the herparin-binding domain of the fibronectin molecule, with at least one of Y and Z being said polypeptide.

Description: This invention relates to the use of a particular polypeptide for the manufacture of a medicament for the therapeutic application of cancer. The polypeptide corresponds to a particular functional domain in the fibronectin (hereinafter referred to as FN) molecule, and it has physiological activities. There are a variety of anticancer agents, including alkylating agents, anticancer antibiotics, antimetabolites and alkaloids. At present, there are several dozens of anticancer agents which can be used clinically. However, in general, these anticancer agents are toxic and have other strong side effects, and the clinical effects cannot be further improved because of these drawbacks. This invention utilizes substances present physiologically in the body to provide the use of a polypeptide of formula [IV] (below) for the manufacture of a medicament for the therapeutic application of cancer, with higher safety than has been obtained before. A known anticancer agent contains a pharmacologically effective amount of compound of the following formula [I]: Y-(X)m-Z [I] wherein Y is a hydrogen atom or a polypeptide corresponding to or functionally equivalent to the polypeptide of the cell-binding domain of the FN molecule, X is a spacer, m is 0 or 1, and Z is -OH or a polypeptide corresponding to or functionally equivalent to the polypeptide of the heparin-binding domain of the FN molecule, at least one of Y and Z being said polypeptide. The spacer is an insertion sequence which is used to adjust the distance between the polypeptide molecule or between Y and Z. Any amino acid, polypeptide, or derivative of the like can be used as the spacer provided that it has no effect on the activity of the polypeptide(s) The presence of this spacer does not affect the effect to be obtained by the use of the polypeptide and it is possible to remove the spacer readily by use of the methods of site-specific mutagenesis. During the research work by the inventors of this invention on the functional domains of FN, which is a physiological substance, it has been found that polypeptides with the structure of the Formula [I] have a strong anticancer activity. FN is a dimer made of polypeptides with an S-S bond in the vicinity of the C-terminal and with a molecular weight of about 500,000. Its sequence of amino acids has repeating structures named types I, II and III. It also has domains with a variety of functions such as binding activity with cells, collagen, heparin, fibrin, and so on. We have done research work on the novel use of the polypeptide of the functional domains of FN and as a result have found that the cell-binding polypeptide, the heparin-binding polypeptide, and the chimera polypeptide of the cell-binding polypeptide and the heparin-binding polypeptide all have strong anticancer activity. Examples of the polypeptides which correspond to or are functionally equivalent to the polypeptide of the cell-binding domain of the FN molecule, which polypeptides have cell-binding activity include those polypeptides with cell-binding activity disclosed in our Japanese Laid-Open Patent Applications Nos. 180900/89 (U.S. Patent No. 5049658), 206998/89 (U.S. Patent No. 5045631), 97397/90 (U.S. Patent No. 5102988) and 152990/90 (U.S. Patent No. 5136023). These polypeptides with cell-binding activity can be prepared efficiently by the methods of genetic engineering. Below, an example will be given in detail concerning the polypeptide with 274 amino acid residues. The polypeptide Pro¹²³⁹-Asp¹⁵¹² with 274 amino acid residues has the structure shown in the following Formula [II]: Its method of preparation is described in Japanese Laid-Open Patent Application No. 97397/90 (U.S. Patent No. 5102988) and will be concretely explained below. In this specification, the numbers used to label the amino acids are the numbers of the amino acid residues from the N-terminal of the amino acid sequence obtained by translation of the cDNA sequence of FN in the EMBL Data Bank. The polypeptide Pro¹²³⁹-Asp¹⁵¹² with 274 amino acid residues is the same as the polypeptide Pro¹²³⁹-Met¹⁵¹⁷ with 279 amino acid residues for which a patent has already been applied (Japanese Laid-Open Patent Application No. 206998/89: U.S. Patent No. 5045631), except that five amino acid residues on the C-terminal have been deleted. The preparation of the polypeptide Pro¹²³⁹-Asp¹⁵¹² with 274 amino acid residues can be done readily with the use of the plasmid pTFD707 which codes for the polypeptide Pro¹²³⁹-Met¹⁵¹⁷ with 279 amino acid residues. A plasmid coding for the polypeptide Pro¹²³⁹-Asp¹⁵¹² with 274 amino acid residues can be prepared by the replacement of the codon AAA for Lys¹⁵¹³, which is the fifth residue from the C-terminal of the polypeptide with 279 amino acid residues, with the stop codon TAA. For this purpose, the methods of site-specific mutagenesis can be used. Cells of Escherichia coli JM109/pTF7221 into which a plasmid that expresses the polypeptide Pro¹²³⁹-Asp¹⁵¹² with 274 amino acid residues has been introduced have been deposited at the Fermentation Research Institute of the Agency of Industrial Science and Technology, Japan, as FERM BP-1915. The purification of the polypeptide with cell-binding activity from the recombinant cells can be done, for example, as follows. The cell pellet is suspended in a buffer, the suspension is sonicated, and the soluble and insoluble fractions are separated. The insoluble fraction is solubilized in a buffer that contains 7 M urea. These two soluble fractions are pooled and put on a column of Sepharose 4B bound with antibody specific for the cell-binding domain of FN for purification by affinity chromatography. Elution is done with a buffer with a pH in the vicinity of 2.3. Immunoblotting is used to obtain the desired fraction, by which means the polypeptide with cell-binding activity is obtained. When necessary, FPLC and HPLC can be used for the purification. An example of the polypeptide which corresponds to or is functionally equivalent to the polypeptide of the heparin-binding domain of the FN molecule, which polypeptide has heparin-binding activity includes the polypeptide with heparin-binding activity disclosed in our Japanese Patent Application No. 131453/89 (U.S. Patent No. 5198423). This application discloses the polypeptide Pro¹²³⁹-Ser¹⁵¹⁵-Met-Ala¹⁶⁹⁰-Thr¹⁹⁶⁰, of formula [IV], below. The polypeptide with heparin-binding activity can be prepared efficiently by the methods of genetic engineering. Below, an example will be given in detail concerning the polypeptide with 271 amino acid residues. The polypeptide Ala¹⁶⁹⁰-Thr¹⁹⁶⁰ with 271 amino acid residues has the structure shown in the following Formula [III]: Its method of preparation is disclosed in our Japanese Patent Application No. 131453/89 (U.S. Patent No. 5198423) , and will be explained in detail below. The plasmid pLF2435 which carries the cDNA fragment coding for the heparin-binding domain of human FN is obtained by the reconstruction of the plasmids pLF2, pLF3, pLF4 and pLF5 described in Biochemistry, vol. 25, 4936-4941 (1986). The desired cDNA fragment is cleaved from this plasmid pLF2435 by the use of restriction enzymes, and DNA ligase is used to link synthetic DNA that contains an initiation codon at its 5' end and synthetic DNA that contains a termination codon at its 3' end. Then, by linkage with an appropriate expression vector, it is possible to construct a plasmid pHD101 that expresses the polypeptide Ala¹⁶⁹⁰-Thr¹⁹⁶⁰ that has the 271 amino acid residues of the heparin-binding domain. As the expression vector, any of the known expression vectors can be used. For example, it is possible to obtain satisfactory results with the use of pUC118N/pUC119N (FEBS Letters, vol. 223, 174-180, 1987) or its derivatives. By the introduction of this plasmid into cells of E . coli , the heparin-binding polypeptide is expressed, and its properties can be investigated. Cells of E . coli HB101/pHD101 into which a plasmid that expresses the polypeptide Ala¹⁶⁹⁰-Thr¹⁹⁶⁰ with 271 amino acid residues has been introduced have been deposited at the Fermentation Research Institute of the Agency of Industrial Science and Technology, Japan, as FERM BP-2264. The purification of the polypeptide with heparin-binding activity from the recombinant cells can be done, for example, as follows. Recombinant E . coli cells are cultured in a medium such as L-broth and the cells are collected and treated by sonication. The sonicated cell extract is centrifuged, and the supernatant is obtained. The supernatant is first dialyzed and then passed through a DEAE ion-exchange column. The eluent is then treated by CM ion-exchange chromatography and/or affinity chromatography on heparin-agarose, or the like. A chimera polypeptide made of the polypeptide with cell-binding activity and the polypeptide with heparin-binding activity is a polypeptide which contains the polypeptide corresponding to or equivalent functionally to the polypeptide of the cell-binding domain of the FN molecule bound directly or by means of a spacer to the polypeptide corresponding to or equivalent functionally to the polypeptide of the heparin-binding domain of the FN molecule. In making an example of such a polypeptide, the inventors of this invention have used the methods of genetic engineering to produce efficiently a polypeptide with 549 amino acid residues that is disclosed in the detailed description of our Japanese Patent Application No. 131453/89 (U.S. Patent No. 5198423). The polypeptide with 549 amino acid residues will be described in detail below. The polypeptide Pro¹²³⁹-Ser¹⁵¹⁵-Met-Ala¹⁶⁹⁰-Thr¹⁹⁶⁰ with 549 amino acid residues is of the following Formula [IV]: The present invention provides the use of the polypeptide of formula IV for the manufacture of a medicament for the therapeutic application of cancer, as claimed in claim 1. Its method of preparation is disclosed in our Japanese Patent Application No. 131453/89 (U.S. Patent No. 5198423) , and will be explained in detail below. The plasmid pTF7021 which codes for the polypeptide Pro¹²³⁹-Met¹⁵¹⁷ with 279 amino acid residues is disclosed in our Japanese Laid-Open Patent Application No. 206998/89 (U.S. Patent No. 5045631). . An Nco I site is introduced immediately before the termination codon of the coding region within the plasmid pTF7021, and this results in the construction of pTF7520, which codes for Pro¹²³⁹-Ser¹⁵¹⁵-Met. An Nco I- Hin cII fragment which contains the coding region for the heparin-binding polypeptide is isolated from pHD101 and inserted into Nco I- Hin cII site of pTF7520, which gives a recombinant plasmid that expresses a polypeptide with 549 amino acid residues in which the cell-binding domain and the heparin-binding domain of FN are linked. This plasmid has in its linkage site the DNA sequence that codes for the methionine residue that originated from the Nco I site and that acts as the linker. The presence of this linker does not affect the results obtained with the use of this invention, and it is possible to remove the linker readily by use of the methods of site-specific mutagenesis, when necessary. Cells of E . coli HB101/pCH101 into which a plasmid which expresses the polypeptide Pro¹²³⁹-Ser¹⁵¹⁵-Met-Ala¹⁶⁹⁰-Thr¹⁹⁶⁰ with 549 amino acid residues have been deposited at the Fermentation Research Institute of the Agency of Industrial Science and Technology, Japan, as FERM BP-2799. The culture of E. coli cells which carry this plasmid under appropriate conditions causes the accumulation within the cells of the desired polypeptide. The purification of the desired polypeptide can be done, for example, as follows. Recombinant E . coli cells are cultured in a medium such as L-broth, and the cells are collected and treated by sonication. The sonicated cell extract is centrifuged, and the supernatant is obtained. The supernatant is first dialyzed and then passed through a DEAE ion-exchange column. Steps of CM ion-exchange chromatography and/or heparin-agarose affinity chromatography, etc., are done. By these steps, it is possible to purify the desired polypeptide. When the functional polypeptide is used in the manufacture of pharmaceutical preparation, it can be prepared in the ordinary way with a suitable known carrier for use in pharmaceutical preparations, for administration by the oral route or other routes. As the other ingredients and as the carrier, any approved substances for pharmacologial use can be chosen, and the route of administration and the method of administration are chosen depending on their kind and on the composition. For example, for use as a liquid carrier, water, alcohols, soybean oil, olive oil, mineral oils, animal oils, vegetable oils, or synthetic oils can be used. As a solid carrier maltose, sucrose, and other sugars, amino acids, hydroxypropylcellulose and other cellulose derivatives, magnesium stearate and other salts of organic acids, etc., can be used. When the agent is to be injectable, the liquid for the making of solution can be physiological saline, suitable buffers, solutions of sugars such as glucose, inositol, mannitol, lactose, etc., and glycols such as ethylene glycol and polyethylene glycol. In addition, sugars such as inositol, mannitol, lactose, sucrose, etc. and amino acids such as phenylalanine can be used together with a vehicle in a lyophilized preparation, and at the time of administration, the lyophilized preparation call be dissolved in an appropriate solution for use in injection, such as, for example, sterilized water, physiological saline, glucose in solution, an electrolyte solution, a solution of amino acids, or other liquids for use in intravenous administraion. The amount of the polypeptide to be incorporated in the pharmaceutical preparation depends on the preparation in question, but in general, it is preferred that the amount be 0.1-100% by weight, and more preferably, that it be 1-98% by weight. For example, when a solution for injection is being made, it is usually preferred that the amount of this polypeptide be 0.1-30% by weight, and more preferably 1-10% by weight. When administration is to be by the oral route, as well as the solid carriers or liquid carriers mentioned above, it is possible for the preparation to be in a form of a tablet, capsule, powder, granules, liquid, dry syrup, etc. In general, when the preparation is to be in the form of a capsule, granules, or powder, the amount of the active ingredient should be 5-100% by weight, and preferably 25-98% by weight. The amount of a single dose depends on the age, weight, and signs and symptoms of the patient, and also on the purpose of treatment, etc., but in general, a therapeutic dose by a non-oral route is 1-100 mg/kg per day and by the oral route, 5-500 mg/kg per day. Acute toxicity: When C57BL/6 mice or CDF₁ mice were used in a test of the toxicity of the polypeptide with 274 amino acid residues, the polypeptide with 271 amino acid residues, or the polypeptide with 549 amino acid residues, no signs of toxicity were found when 100 mg/kg of a polypeptide was given by intravenous injection. This invention will be explained in more detail by reference to the following examples. However, this invention is not to be taken to be limited to these examples. In all of these examples, "parts" refers to "parts by weight". Reference Example 1. CDF₁ mice, in groups with 10 mice each, were injected intravenously with either 4 x 10⁴ cells of the lymphoma cell line L5178Y-ML25 or else with a mixture of 4 x 10⁴ cells of the lymphoma cell line of L5178-ML25 plus 250 µg of specified polypeptide of formula [II], [III] or [IV]. The prolongation of life was calculated for the groups that received one of the polypeptides as a percentage of the mean days of survival of the control group after the transplantation by intravenous injection of the lymphoma cells, as follows: Table 1 shows the number of days each mouse in each group survived, up to day 26 after transplantation of lymphoma cells, together with the survival ratio (number of mice surviving until day 26/number of mice in that group), the mean days of survival, and the percent prolongation of life. As shown above, the administration of the polypeptides had an anticancer effect. Reference Example 2. (1) Some 5 x 10⁵ cells of the highly metastatic cell line B16-BL6 melanoma cells were injected in a volume of 0.05 ml subcutaneously into the foot pad of the right back foot of C57BL/6 mice, which were divided into groups of five, and 21 days after the transplantation, the primary melanoma was removed surgically. Some mice were injected intravenously once daily with 0.1 ml of solution of one of the polypeptides of formulae [II] and [IV] dissolved at the concentration of 1 mg/ml in phosphate-buffered saline (PBS) on one of the following schedules: on days 7, 9, 11, 13, 15, 17, and 19 after the transplantation, which was before the surgery, or on days 22, 24, 26, 28, 30, 32, and 34 after the transplantation, which was after the surgery. The control mice were injected with PBS on one of these two schedules. The mice were killed and their lungs were removed surgically on day 35 after the transplantation. The number of metastatic nodules on the lung surfaces was counted under a dissection microscope. The results are shown in Tables 2 and 3. Results with administration before surgical removal of primary melanoma Compounds Mean metastases per lung ± SD Controls 53 ± 15 Polypeptide with 274 amino acid residues 22 ± 4 Polypeptide with 549 amino acid residues 34 ± 16 Results with administration after surgical removal of primary melanoma Compounds Mean metastases per lung ± SD Controls 106 ± 23 Polypeptide with 274 amino acid residues 43 ± 33 Polypeptide with 549 amino acid residues 32 ± 22 As shown above, the polypeptides inhibit the metastasis of melanoma to the lungs. (2) CDF₁ mice, in groups of 5 mice each, were injected intravenously with 4 x 10⁴ cells of the lymphoma cell line L5178Y-ML25. The cells were injected in solution together with the polypeptide with 274 amino acid residues, the polypeptide with 271 amino acid residues, or the polypeptide with 549 amino acid residues at different concentrations. The controls were injected with the lymphoma cells only. On day 14 after the transplantation of lymphoma cells, the mice were killed, and their livers and spleens were removed, weighed, and compared. Results are in Table 4. Results with administration Compounds Dose of compound µg/mouse Mean liver weight g ± SD Mean spleen weight g ± SD Controls 4.53 ± 0.50 0.24 ± 0.04 Polypeptide with 274 amino acid residues 100 3.78 ± 0.62 0.23 ± 0.03 250 4.14 ± 0.84 0.22 ± 0.03 500 3.84 ± 0.63 0.20 ± 0.02 Polypeptide with 271 amino acid residues 100 2.72 ± 0.80 0.17 ± 0.05 250 2.24 ± 0.75 0.14 ± 0.05 500 1.42 ± 0.35 0.11 ± 0.02 Polypeptide with 549 amino acid residues 100 0.90 ± 0.05 0.08 ± 0.01 250 0.90 ± 0.07 0.08 ± 0.01 500 0.90 ± 0.08 0.08 ± 0 As shown above, in the groups of mice treated with the polypeptides metastasis of lymphoma cells to the liver and the spleen was inhibited. Comparison Example 1 To 30 parts of the polypeptide with 274 amino acid residues, PBS was added for the total weight of 2000 parts, and the solution was sterilized by filtration through a Millipore GS filter. Then 2 g of the filtrate obtained was put into a 10 ml vial and lyophilized. This gave a vial containing 30 mg of lyophilized polypeptide for use in injection. Comparison Example 2 To 30 parts of the polypeptide with 271 amino acid residues, PBS was added for the total weight of 2000 parts, and the solution was sterilized by filtration through a Millipore GS filter. Then 2 g of the filtrate obtained was put into a 10 ml vial and lyophilized. This gave a vial containing 30 mg of lyophilized polypeptide for use in injection. Example 1 To 30 parts of the polypeptide with 549 amino acid residues, PBS was added for the total weight of 2000 parts, and the solution was sterilized by filtration through a Millipore GS filter. Then 2 g of the filtrate obtained was put into a 10 ml vial and lyophilized. This gave a vial containing 30 mg of lyophilized polypeptide for use in injection. Comparison Example 3 To 50 parts of the polypeptide with 274 amino acid residues, 600 parts of lactose, 330 parts of crystalline cellulose, and 20 parts of hydroxypropylcellulose were added and thoroughly mixed. The mixture was compressed in a roller-compactor, and pulverized so as to pass through meshes sized from 16-60 mesh, giving granules. Comparison Example 4 To 50 parts of the polypeptide with 271 amino acid residues, 600 parts of lactose, 330 parts of crystalline cellulose, and 20 parts of hydroxypropylcellulose were added and thoroughly mixed. The mixture was compressed in a roller-compactor, and pulverized so as to pass through meshes sized form 16-60 mesh, giving granules. Example 2 To 50 parts of the polypeptide with 549 amino acid residues, 600 parts of lactose, 330 parts of crylstalline cellulose, and 20 parts of hydroxypropylcellulose were added and thoroughly mixed. The mixture was compressed in a roller-compactor, and pulverized so as to pass through meshes sized from 16-60 mesh, giving granules. The polypeptides of formulae [II], [III] and [IV] are useful as anticancer agents and are safe because they are substances related to substances present physiologically in the body. The substances can be obtained in large amounts, as the result of genetic engineering, which enhances their usefulness.

Claims: Use of a polypeptide for the manufacture of a medicament for the therapeutic application of cancer, wherein said polypeptide has the sequence of the following Formula [IV]:

Inventors: AZUMA ICHIRO, KATO IKUNOSHIN, KIMIZUKA FUSAO, SAIKI IKUO, AZUMA, ICHIRO, KATO, IKUNOSHIN, KIMIZUKA, FUSAO, SAIKI, IKUO
Applicants: TAKARA SHUZO CO, TAKARA SHUZO CO. LTD.
Application year: 1990
Main classification: C07K13
IPC codes: C07K14, A61K38