PROCESS FOR TREATMENT OF LIGNOCELLULOSIC PULP

Abstract: The process comprises treatment of the pulp with an alkaline xylanase at pH values above 7.5 with subsequent treatment of the thus treated pulp with chlorine with a multiple for active chlorine of 0.20 or less in the first chlorination stage. In this manner the necessary time for the enzymatic pretreatment can be reduced, and furthermore the need for the amount of active chlorine in the first chlorination stage is reduced.

Description: PROCESS FOR TREATMENT OF LIGNOCELLULOSIC PULP A common stage in the treatment of wood chips for paper production is chemical pulping, e.g. the so-called Kraft process which is an alkaline sulphate cooking of the wood chips. The native wood chips contain around 30% lignin, and at the end of the chemical cooking process less than 5% of the lignin compounds is still left in the pulp. Due to the strong brown color of the remaining lignin and its tendency to darken in UV light or by oxidation this has to be removed in order to obtain a white pulp without the tendency to color reversion. The brown color can be removed by a multistage bleaching using e.g. chlorine and/or chlorine dioxide. However, due to the ever increasing environmental concern, the dosage of chlorine and/or chlorine dioxide has to be kept at a minimum, and for that reason the enzymatic treatment of the Kraft cooked pulp has been introduced, vide e.g. The third International Conference on Biotechnology in the Pulp and Paper Industry, Stockholm, 16-19.6, 1986, page 67-69. Hitherto the enzymatic treatment has been carried out at acid pH with hemicellulases with a pH optimum in the acid pH-region, vide 4th International Symposium Wood and Pulping Chemistry, Paris, 22-30 April, 1987, Vol. 1, page " 151-154. The fact that the enzymatic treatment hitherto has to be carried out at an acid pH is a disadvantage, because the alkaline Kraft pulp has to be acidified, which requires big amounts of chemicals, and also, it disturbs the salt balance, which can be a problem in the recovery system of the pulp mill. For certain chemical pulping processes, e.g. Kraft pulping, it would be highly advantageous if the enzymatic treatment could be carried out at alkaline pH, thus avoiding the need for neutralization of the pulp, and making the enzyme process directly adaptable to the existing process hardware. Surprisingly, according to the invention it has been found that alkaline xylanases exhibiting an excellent bleachability improving effect actually exist. Furthermore, it has been found that the time period necessary for enzymatic pretreatment can be shortened considerably with such alkaline xylanases, and also, use of the alkaline xylanases is accompanied by a reduced need for active chlorine in the bleaching stages compared to the acid treatments described in the literature and/or the brightness is improved. Thus, the process for treatment of lignocellulosic chemical pulp is characterized by the fact that the lignocellulosic pulp is treated with an alkaline xylanase at a pH above 7.5, whereafter the thus treated lignocellulosic pulp is treated with chlorine at an active chlorine multiple of 0.20 or less in the first chlorination stage. In this specification with claims an alkaline xylanase is a hemicellulase which exhibits a high xylanase activity at high pH values, as specified in more detail in the following. In this specification with claims an alkaline xylanase is defined as a hemicellulase which at a pH of 9.0 retains at least 10% of its maximum xylanase activity (activity at optimum pH) . The xylanase activity at a given pH is measured as the Kappa no. reduction obtained in the experimental procedure described below. Experimental conditions: The substrate is a well washed Birch Kraft pulp having a Kappa no. of 15 to 25 and a viscosity of 1200-1500 dm 3 /kg (viscosity measured in cupri ethylene diamine solution according to the SCAN standard SCAN-CM 15:88) . Buffer solutions: In the pH range from 5.8 to 8.0 a 0.05 M potassium dihydrogen ' ■ phosphate buffer is used, and in the pH range from 8.0 to 10.8 a 0.0125 M borate buffer is used. Procedure: A sample of pulp is disintegrated according to the Scan standard (SCAN-C18:65) . A buffer solution having the desired pH is used in the pulper instead of water. After pulping the pulp is drained on a wire screen to a concentration of approximately 25%. The pulp is then diluted to 10% dry substance using a buffer solution of the desired pH, which contains the xylanase in solution. 500 EXU- units of xylanase is added per kg of dry pulp. EXU is an abbreviation for endoxylanase activity units, to be defined later. The pulp and xylanase is mixed thoroughly and placed on a water bath at 50°C for 3 hours. After the 3 hours of enzyme treatment the pulp is washed with water on a wire screen and the Kappa no. of the well-washed pulp is determined. The above described procedure is repeated as a control experiment, i.e. without addition of xylanase. At a given pH the reduction in Kappa no. is calculated as (Kappa no. of control minus Kappa no. of xylanase treated pulp) . If less xylanase than 10 EXU/kg of dry pulp is used, no significant improvement of the bleacheability of the pulp can be detected. The alkaline hemicellulase preparations usually contain cellulase activity as a side activity. In order to avoid decomposition of the cellulose to any undue extent, the cellulase activity should be small in comparison to the xylanase activity. Thus, an enzyme dosage giving rise to a cellulase activity of more than 1000 EGU units/kg of dry pulp (the EGU unit for cellulase activity to be defined later) should preferably not be used. The EXU activity unit for xylanase activity is defined in AF-293.9/1, which can be obtained on request from Novo Nordisk a/s, Novo Alle, DK- 2880 Bagsvaerd, Denma'rk. The EGU activity unit for cellulase activity is defined in AF-275/1, which can be obtained on request from Novo Nordisk a/s, Novo Alle, DK-2880 Bagsvaerd, Denmark. As indicated, the treatment with the alkaline xylanase precedes the chlorine treatment; however, the chlorine treatment does not necessarily follow directly after the treatment with the alkaline xylanase. The chlorine multiple is the factor which multiplied by the Kappa no. of the pulp gives the dosage of active chlorine in the first bleaching stage as % (w/w) on dry pulp. The Kappa no. is a measure of the lignin content and is defined in SCAN-C 1:77 (Scandinavian Pulp, Paper and Board Testing Committee) . Due to the fact that the enzyme preparations used according to the invention are hemicellulase complexes, these preparations usually also contain other polyose degrading enzymes as side activities to the xylanase activity, e.g. galactomannases and arabinosidases. The xylanase activity is the key activity, but other hemicellulase activities are also desirable for obtaining the bleacheability improving effect. Thus, the process for treatment of lignocellulosic Kraft pulp is a new combination of two items: 1) the use of alkaline xylanases, and 2) the use of a low multiple, lower than the commonly used multiple in traditional bleaching processes in the range of 0.20-0.25. Summing up, the process for bleaching of enzymatically treated lignocellulosic pulp according to the invention is accompanied by the following advantages: 1) there is no need for neutralization of the alkaline pulps, e.g. Kraft brown stock. 2) The time for enzyme treatment can be shortened considerably. 3) The low multiple means that the chlorine addition is reduced, which in itself is accompanied by a number of « advantages (economic, easier treatment of waste water, smaller amounts of toxic substances) . 4) Higher brightness. In a preferred embodiment of the process according to the invention the xylanase treatment is performed at temperatures between 40 and 100°C, preferably between 40 and 80°C, more preferably between 50 and 70°C. The temperature interval of 40-100°C is the normal pulp temperature at this stage, and it has been found that the activity and stability of the alkaline xylanases used in the invention is satisfactory in this interval. In a preferred embodiment of the process according to the invention the xylanase treatment is performed over a period of 15 minutes to 24 hours, preferably between 30 minutes and 5 hours, most preferably between 30 minutes and 3 hours. With reasonable xylanase activities compatible with a sound commercial economy it has been found that the xylanase has performed its hydrolysing activity to the desired degree of hydrolysis in this short period of time, whereas 12-24 hours is the usual prior art time period for the xylanase treatment, vide Viikari, L. et al. , Third International Conference on Biotechnology in the Pulp and Paper Industry, Stockholm 16-19.6.1986. In a preferred embodiment of the process according to the invention the xylanase treatment takes place at a consistency of 5-35%, preferably 8-25%, most preferably 8- 15%. The consistency is the dry matter content of the pulp. A pulp with a consistency above 35% is difficult to mix effectively with the enzyme preparation, and a pulp with a consistency below 5% carries too much water, which is a disadvantage from an economic point of view. In a preferred embodiment of the process according to the invention the xylanase is producible by means of the microorganisms Malbranchea cinnamomea , Humicola insolens. Bacillus pumilus. Bacillus stearothermophilus . Thermonmonospora fusca or Streptomyces olivochromocrenes. These microorganisms are able to produce alkaline xylanases, which can be used in 'the process according to the invention. Other microorganisms which are able to produce alkaline xylanases, can be located by means of the routine test described earlier in this specification. In a preferred embodiment of the process according to the invention an (EOP) treatment is introduced between the xylanase treatment and the chlorine treatment. An (EOP) stage is an (EO) extraction stage reinforced with hydrogen peroxide, as described by e.g. Helming, 0. et al. , Tappi Journal, Vol 72, No. 7 (1989), p. 55-61. By means of this supplementary process the Kappa no. of the enzyme treated pulp is lowered considerably more than what is normally the case after an (EOP) stage. In a preferred embodiment of the process according to the invention the chlorine multiple is between 0.10 and 0.20. Even a small reduction in chlorine multiple is a significant improvement, as a small reduction in the amount of chlorine used significantly cuts back on the formation of chlorinated organic compounds, e.g. chlorinated dioxins, vide Kringstad, K. Sv. Papperstidning, Vol. 92, no. 6, 1989, p. 42-44. In order to illustrate the process according to the invention reference will be made to the following examples. The bleaching stages are abbreviated as the C, D, and E processes, the C process being the chlorine bleaching stage, the D process being the chlorine dioxide bleaching stage, and the E process being the alkaline extraction stage, vide The Bleaching of Pulp, Rudra P. Singh. Tappi Press, 1979. The ISO-brightness is defined in International Standard, ISO, 2470/1977(E) , Paper and Board - Measurement of diffused blue reflectance factor (ISO Brightness) . EXAMPLE 1 Production of Malbranchea cinnamomea xylanase Malbranchea cinnamomea fsvn. M. sulfurea, M. pulchella var. sulfurea) culture UAMH 2485 (UAMH indicates University of Alberta Mold Herbarium culture collection, Arberta, Canada) was maintained on YPG agar slants at 45°C. Composition of YPG-agar: Glucose 15 g/1 Yeast extract (from Difco) 4 g/1 K 2 HP0 4 1 g/1 MgS0 4 , 7H 2 0 0.5 g/1 Agar 20 g/1 Autoclaved at 121°C for 20 minutes 120 shake flasks with 150 ml XYH-4 medium each inoculated from YPG-agar slants and cultivated (at 250 rp . with appr. 2 cm amplitude) at 45°C for 6 days. Composition of XYH-4: Yeast Extract (from Difco) 5 g/1 KH 2 P0 4 5 g/1 Xylose 10 g/1 Pluronic 61 0.1 ml/1 pH is adjusted to 6 (by NaOH/ H 2 S0 4 ) Autoclaved at 121°C for 40 minutes The mycelium was removed from the broth by filtration through a 100 μ nylon filter and a 10 μm nylon filter. The filtrate (a total of 9,2 1) was concentrated by ultrafiltration (and washed 3 times with one volume water) to 500 ml by a Pellicon ultrafiltration apparatus from Millipore with a 10,000 MW cut-off filter sheet). The resulting con¬ centrate was freeze dried, whereby 6,25 g of powder was generated. EXAMPLE 2 Production of Bacillus pumilus xylanase Bacillus pumilus culture DSM 6124 (deposited on July 23, 1990 at Deutsche Sammlung von Mikroorganismen under 5 the conditions of the Budapest Treaty) was maintained on A3- medium, at 37°C. A3-medium: g/i Peptone, Difco 6.0 10 Yeast Extract 3.0 Pepticase 4.0 Beef Extract 1.5 Glucose 1.o Agar, Merck 20.0 15 H 2 0 1000 ml pH adjustment to 7,3 before autoclaving. Autoclaving 25 min./12l°C. 120 shake flasks with 150 ml XYL-8 medium each inoculated from A3-agar slants were cultivated for 4 days, 2037°C, 250 rpm with appr. 2 cm amplitude. XYL-8 medium: g/i Bacto-peptone Difco 10.0 Yeast extract 10.0 25 K 2 HP0 4 15.0 MgS0 4 .7H 2 0 0.5 KC1 0.1 FeS0 4 .7H 2 0 0.01 Beech xylan Lenzing 6.0 30 pH adjustment to 7.0 before autoclaving. Autoclaving 25 min./12l°C. The broth was centrifuged for half an hour at 4.000 rp (SORVALL RC-3B centrifuge with a 6000 A rotor) The supernatant, 7.3 1, was filtered through a 10 μm nylonfilter, and concentrated by ultrafiltration by means of a Pellicon equipment from Millipore with a 10.000 MW cut-off membrane and washed 2 times with one volume of water. This resulted in 540 ml of concentrate. The concentrate was then lyophilized, whereby 8.8 g of powder was generated. EXAMPLE 3 Production of Bacillus stearothermophilus xylanase. Use was made of Bacillus stearothermophilus culture NR. B-18659 (Agricultural Research, Culture Collection, Peoria, Illinois, USA) . Fed batch fermentation with the above Bacillus stearothermophilus culture was performed by means of a batch medium containing xylan along with a 50% (w/w%) xylose feed. The pH was controlled not to drop below pH 6.5 by means of 4N NH 4 OH. The following ingredients were used: The feed was started at 22 hours at a constant rate of 3.3 ml/l/hr. The feed was stopped at 90 hours and the fermentation was stopped at 95 hours. The enzyme preparation is a liquid preparatδpm obtained after ultrafiltration with 10,000 molecular weight cut-off (MWCO) filter- and stabilization with 20% sorbitol. EXAMPLE 4 Production of Humicola insolens xylanase. Humicola insolens culture ATCC 22082 (ATCC indicates American Type Culture Collection, Rockville, MD, USA) was maintained on YPG agar slants at 37°C. Composition of YPG-agar: Glucose 15 g/1 Yeast extract (from Difco) 4 g/1 K 2 HP0 4 1 g/1 MgS0 4 , 7H 2 0 0.5 g/1 Agar 20 g/1 Autoclaved at 121°C for 20 minutes 100 shake flasks with 150 ml XYH-9 medium each inoculated from YPG-agar slants and cultivated (at 250 rpm. with appr. 2 cm amplitude) at 37°C for 5 days. Composition of XYH-9: Wheat bran 50 g/1 Corn steep liqueur 30 g/1 NaH 2 P0 4 5 g/1 pH is adjusted to 6 (by NaOH/H 2 S0 4 ) Autoclaved at 121°C for 40 minutes The broth was centrifuged for 35 minutes at 4000 rpm by means of a Sorval RC-3B centrifuge with a 6000 A rotor. The supernatant (a total of 5,4 1) was filtered through a nylon filter (10 μm) and concentrated by ultrafiltration (and washed 3 times with one volume water) to 760 ml by a Pellicon ultrafiltration apparatus from Millipore with a 10,000 MW cut-off filter sheet). The resulting concentrate was freeze dried, whereby 19,1 g of powder was generated. ' EXAMPLE 5 Production of Thermonmonospora fusca xylanase Thermonmonospora fusca ATCC 27730 (American Type culture Collection) was maintained on A3-medium at 37°C. A3 medium: g/i Peptone, Difco 6.0 Yeast Extract 3.0 Pepticase 4.0 Beef Extract 1.5 Glucose 1.0 Agar, Merck 20.0 H 2 0 1000 ml pH adjustment to 7.3 before autoclaving. Autoclaving 25 min./121°C. Thermonmonospora fusca was grown on A3-slants for 5 days, 37°C. The culture was resuspended in sterile water and inoculated in shake flasks with medium XYL-lOa. Shake flasks of 100 ml were grown for 2 days, 45"C, 250 rpm, amplitude appr. 2 cm. XYL-lOa medium: g/i Soya-grit 20. 0 Na-caseinat 10. 0 Na 2 HP0 4 9. 0 Beech xylan Lenzing 3.0 Pluronic 61 L 0.1 ml H 2 0 1000 ml pH adjustment to 7.5 before autoclaving. Autoclaving 30 minutes/121°C. Hereafter a 3 1 Applicon fermentor, filled to 2.5 1 with XYL-lOa substrate was incubated with 5% of the fermentation volume. The fermenter was run for 4 days, 45°C, 700 rpm, 2 NL/min. The culture broth was centrifuged for 30 min. at 4000 rpm Sorwall RC-3B with a 6000 A rotor, and hereafter filtered through a 10 μm filter. The resulting volume of 2470 ml was then ultrafiltered through a 10.000 MW cut-off membrane using Pellicon ultrafiltration equipment from Millipore. Result 275 g of liquid. EXAMPLE 6 Production of Streptomvces olivochromo enes xylanase Streptomvces olivochromoqenes DSM 6126 (deposited on July 23, 1990 at Deutsche Sammlung von Mikroorganismen under the conditions of the Budapest Treaty) was maintained on A3-medium at 30°C. A3 medium: g/1 Peptone, Difco 6.0 Yeast Extract 3.0 Pepticase 4.0 Beef Extract 1.5 Glucose 1.0 Agar, Merck 20.0 H 2 0 1000 ml pH adjustment to 7.3 before autoclaving. Autoclaving 25 min./121°C. Streptomvces olivochromoαenes was grown on A3- slants for 5 days, 30°C. The culture was resuspended in sterile water and inoculated in shakeflasks with medium XYL- 10. Shake flasks of 100 ml were grown for 2 days, 30°C, 250 rpm, appr. amplitude 2 cm. XYL-10 medium: g/i Soya-grit 20.0 Na-caseinat 10.0 Na 2 HP0 4 9.0 Beech xylan Lenzing 3.0 Pluronic 61 L 0.1 ml H 2 0 1000 ml pH adjustment to 7.0 before autoclaving. Autoclaving 30 min./121°C. Hereafter a 10 1 Chemap A fermenter, filled to 7 1 with XYL-10 substrate was incubated with 5% of the fermentation volume. The fermenter was run for 5 days, 30°C, 600 rpm, 1 NL/min. The culture broth is centrifuged for 30 min at 4000 rpm Sorwall RC-3B with a 6000 A rotor, and hereafter filtered through a 10 μm filter. The resulting volume of 7000 ml was then ultrafiltered through a 10,000 MW cut-off membrane using Pellicon ultrafiltration equipment from Millipore. Result: 398 g of liquid. EXAMPLE 7 Oxygen delignified hardwood is treated with a hemicellulase preparation from Malbranchea cinnamomea (produced according to the procedure in Example 1) at pH=8.0, 50°C and a consistency of 10% for 3 hours. An enzyme dosage of 30.0 kEXU/kg is used. After the enzyme treatment the pulp is washed and the kappa numbers determined. A control sample of pulp is treated like the enzyme treated sample but without addition of enzyme. After the enzyme treatment the pulps are bleached in a C/D-E bleaching sequence using varying amounts of active chlorine in the C/D-*stage. The bleaching conditions are as follows: C/D-stacfe Time: t = 45 min Temperature: T = 40°C Consistency: DS = 10% Substitution: 30% with C10 2 (as active chlorine) E-stacre Time: t = 1 hr Temperature: T = 60°C Consistency: DS = 10% NaOH dosage: 2.0% (w/w) on dry pulp After the C/D-E stages the kappa number of the pulps are deter-mined. In Table 1 below the kappa numbers after the enzyme treatment are listed. In Table 2 the kappa numbers after the C/D-E stages are listed together with the amounts of active chlorine used for the bleaching together with the active chlorine multiples. Table 1 Kappa number after enzyme treatment Control 11.0 Enzyme 9.2 Table 2 It is seen that the multiple for active chlorine can be reduced below the claimed value of 0.2 after the enzyme treatment and at the same time still giving a lower kappa number after C/D-E than for a control bleached with active chlorine in an amount corresponding to a multiple of 0.22. EXAMPLE 8 An unbleached hardwood brown stock is treated with a hemicellulase preparation from Bacillus pumilus (produced according to the procedure in Example 2) at the following conditions: Time: t = 3 hours Temperature: T = 50°C pH: pH = 8.0 Consistency: DS = 10% Dosage: 715 EXU/kg dry pulp After the enzyme treatment the pulp is washed with water and bleached in a three stage bleaching sequence, C/D- E-D. A control is treated in the same way but without addition of enzyme. Table 3 below shows the kappa numbers of the pulps after the enzyme treatment. Table 3 The C/D-E-D bleaching stages are performed under the following conditions: C/D-sta e Time: t = 20 min Temperature: T = 50°C Consistency: DS = 5% Substitution: 50% with C10 2 (as active chlorine) E-staqe Time: t = 1 hr Temperature: T = 60°C Consistency: DS = 10% NaOH dosage: 2.0% (w/w) on dry pulp D-staqe Time: t = 3 hr Temperature: T = 70°C Consistency DS = 10% Both pulps are bleached to a kappa number of 3.5 after the C/D-E stages. For the control 2.8% (w/w) active chlorine is needed in the C/D-stage to reach this kappa number. For the enzyme treated pulp only 2.38% (w/w) active chlorine is needed to reach a kappa number of 3.5. This corresponds to a reduction in active chlorine of 14.5% for the enzyme treated pulp compared to the control in order to reach the desired kappa number. In the final D-stage both pulps having a kappa number of 3.5 after C/D-E are bleached to their respective brightness ceilings. For the enzyme treated pulp a chlorine dioxide dosage of 0.99% (w/w) is needed to bring the pulp to a brightness of 87.2% (ISO) brightness. For the control a dosage of 1.2% (w/w) chlorine dioxide is needed to reach a final brightness of 85% (ISO) . This shows that the enzyme treatment makes it possible to reduce the dosage of chlorine dioxide in the final* D-stage by 17.5% and at the same time elevate the brightness ceiling of the pulp by a 2.2% ISO brightness. The enzyme treatment of the pulp neither causes strength nor yield loss. EXAMPLE 9 A crude xylanase preparation from Bacillus stearothermophilus (produced according to the procedure in Example 3) was used to pretreat a softwood kraft pulp before a C-E bleaching. The pulp was treated under the following conditions: Pulp: Loblolly Pine kraft brown stock (initial kappa number 24) pH: 8.5 Time: 1 hour Temperature: 60°C Pulp consistency: 3.5% Dose: 1000 EXU/kg dry pulp Pulp and enzyme were mixed by hand in plastic bags and kept at constant temperature in a water bath. A control sample was submitted to the same treatment as described above, but without addition of enzyme. After the enzyme treatment the pulps were bleached under the following conditions: C-staqe Time: t = 45 min Temperature: T = 40°C Consistency: DS = 3.5% E- stage Time : t = l hr Temperature: T = 70°C Consistency: DS = 10% NaOH dosage: 0.5 times the chlorine charge In Table 4 the kappa numbers after the C-E stages are listed both for the enzyme treated pulps and for the controls at four different dosages of chlorine in the first stage. The experiment show that the enzyme treatment at an alkaline pH {8.5} cause a significant drop in kappa number after the C-E bleaching compared to the control. Table 4 It is seen that a kappa number of 7.12 (the kappa- number of the control at a multiple of 0.22) can be obtained after an enzyme treatment by means of a multiple of approx. 0.158 (found by linear interpolation between the experimental values) . This is a reduction in dosage of chlorine of approx. 30% after the enzyme treatment compared to the control. EXAMPLE 10 An unbleached birch kraft pulp was treated with an xylanase rich hemicellulase preparation from Humicola insolens (produced according to the procedure in Example 4) . After the enzyme stage the pulps were bleached in a three stage bleaching sequence, C/D-E-D, to a final brightness of 88% ISO. The pulps were treated under the following conditions: Enzyme Time: t = 3 hours Temperature: T = 50°C Consistency: DS = 10% pH: pH = 9.0 Dosage: 1900 EXU/kg dry pulp C/D-staqe Time: t = 45 min Temperature: T = 40°C Consistency: DS = 5% Substitution: 30% E-staqe Time: t = 1 hr Temperature: T = 60°C Consistency: DS = 10% NaOH dosage: 2% (w/w) on dry pulp D-stage Time: t = 3 hr Temperature: T = 70°C Consistency: DS = 10% Both the enzyme treated pulp and the control were bleached to a kappa number of 3.0 after the C/D-E stages. To obtain this kappa number the active chlorine dosage for the enzyme treated pulp can be reduced by 19% down to 2.24% (w/w) compared to the control. The multiple for active chlorine for the enzyme treated pulp is 0.16. In the final D-stage the control sample reaches the brightness ceiling a 88% (ISO) brightness by means of a dosage of 1.33% w/w C10 2 on dry pulp. The enzyme treated pulp only requires 0.86% (w/w) C10 2 on dry pulp to reach this brightness. By addition of the reduced amounts of bleaching agents in the two bleaching stages (as active chlorine) it is seen that the treatment of the pulp by means of a hemicellulase preparation from H. insolens makes it possible to reduce the required amount of active chlorine after an enzyme treatment by 28% compared to a control when bleaching the pulp to a final brightness of 88% (ISO) . EXAMPLE 11 The enzyme preparation from H. insolens that was used for the experiments described in the Example 10 has also been tested on oxygen delignified softwood. The softwood was treated with the enzyme and afterwards bleached in a C/D-E sequence. The conditions for the enzyme treatment as well as the bleaching were the same as those listed in the above example 10, except for the substitution with chlorine dioxide. The substitution was raised to 50% in this experiment. After the enzyme treatment the entire amount of the pulp was bleached with active chlorine in an amount corresponding to a multiple of 0.2 in the C/D-stage corresponding to a dosage of 3.36% (w/w) of active chlorine. In Table 5 below the kappa numbers after the C/D-E bleaching are listed for four enzyme treatments with varying dosages of enzyme. Table 5 It is seen that the enzyme treatment at all four dosages tested reduces the kappa number of the pulp after the C/D-E bleaching rather significantly compared to the control. 5 When comparing these results with the results presented in Example 7 it is also observed that the hemicellulase preparation shows approximately the same bleach boosting effect on both softwood and hardwood. EXAMPLE 12 0 An unbleached hardwood brown stock pulp was treated with a xylanase containing enzyme preparation from Thermomonospora fusca (produced according to the procedure in- Exa ple 5) and afterwards bleached in a C/D-E bleaching sequence. The conditions for enzyme treatment and bleaching 5 were as follows: Enzyme Time: t = 3 hours Temperature: T = 50°C Consistency: DS = 10% 0 pH: pH = 8.0 and 9.0 Dosage: 200, 400 and 800 EXU/kg of dry pulp C/D-stage Time: t = 45 min Temperature: T = 40°C Consistency: DS = 5% Substitution: 50% E-staqe Time: t = 1 hr Temperature: T = 60°C Consistency: DS = 10% NaOH dosage: %(w/w) NaOH = 0.5 (%(w/w)Cl 2 + %(w/w)C10 2 ) + 0.3 In the C/D-stage the pulps were bleached using an active chlorine multiple of 0.18. After the E stage the kappa numbers of the pulps were determined. The results are listed in Table 6 below for three enzyme dosages at two pH levels for the enzyme treatment. In Table 6 the reduction in kappa number after an enzyme treatment compared to a control treatment is also listed. Table 6 For this enzyme complex good bleach boosting effects are observed both at pH 8 and 9. The results indicate that the effects obtained at a pH of 9 is better than the effects obtained at a pH of 8. EXAMPLE 13 An unbleached hardwood pulp was treated with a xylanase containing enzyme preparation from Streptomyces olivochromoqenes (produced according to the procedure in 5 Example 6) and afterwards bleached in a C/D-E bleaching sequence. The conditions used for both the enzyme treatment and the bleaching are the same as those listed in Example 12. After the C/D-E bleaching the kappa number of the pulps were determined. The results are listed in Table 7 0 below. The percentage reduction of the kappa number after an enzyme treatment compared to the control is also listed. Table 7 15 20 It is observed that good bleach boosting effects are obtained both when the pulp is treated with enzyme at pH 8 and 9. 25 EXAMPLE 14 Hardwood kraft pulp was treated with the same enzyme preparation from Streptomyces as used in Example 13 under the same conditions as listed in Example 12. The pulp was treated with enzyme, 160 EXU/kg of dry pulp, at pH 8 and 309. After the enzyme treatment the samples were bleached to the same kappa number after the C/D-E stages. When bleaching to a kappa number of 3.8 the dosage of active chlorine in the C/D stage can be reduced by 23 and 16 percent compared to a control when enzyme treated at pH 8 and 9, respectively. Afterwards the pulps were bleached to a final brightness of 89% ISO brightness in a D-stage. The strength properties of the fully bleached pulps were tested. The strength of the pulps as well as the yield has not been reduced by the enzyme treatment. In rn-rton*. Application No: PCT/ MICROORGANISMS Optional St * — I In connection with th* mJcroorgenlem referred t* en peso P 8_ . . of th* deβcrtpβen < A. lOtNTIFICATtON OF OIPOWT I Further depoalt* *ra IdentMed en in additional eheet Q> Nun* β( dapoaltary Inβtrtuββfl « DEUTSCHE SAMMLUNG VON MIKROORGANISMEN UND ZELL- KULTUREN GmbH AddreM o< d*potKary Inatttution (Including poatal cod* and country) « Mascheroder Weg lb, D-3300 Braunschweig, Federal Re¬ public of Germany Data ol depoalt » Acceeaion Number • 23 July 1990 DSM 6124 a. ADDITIONAL INDICATIONS * (lea** Wank If not applicable). Thli Information I* continued on a aeøarat* attached iheet ~~~ In respect of those designations in which a European patent is sought, a sample of the deposited micro¬ organism will be made available only by the issue of such a sample to an expert nominated by the person requesting the sample (Rule 28 (4) EPC) until the publication of the mention of the grant of the Euro¬ pean patent or until the date on which the appli¬ cation has been refused or is deemed to be withdrawn. }R WHICH INDICATIONS All MAOI • (H th* indication* are net lor al d**_«naιed State*) D. SIPARATI FURNISHIN* Of INDICATIONS • (lea»e blank H net applicable) Th* indication* Hated below will be aubmltted tβ the International lureau later » (Specify the oβneral nature βl th* Indication* *.f _, " Acceaaion Number oi Depoalt " * ) I. [jξj Thli aheet »ιι received with the international application when filed no be checked by th* raceUint Offkβ) Q Th* data βl receipt (from th* applicant) by the International lureau •• (Author-lie* * Officer) Farm FCT/ΛO/1J (January Ittl) International Application No: PCT/ MICROORGANISMS Optional Sheet in connection with the mkroorganlam referred to on pace _L_= _1Q- . of th* deecnpβen ■ A. IDKNTIFICATION OF DIlOβlT • Further depoalt* are Identified on an additional eheet Q» Name of depositary ina β tu β oa « DEUTSCHE SAMMLUNG VON MIKROORGANISMEN UND ZELL- KULTUREN GmbH Addreea of depoaltary Inatttution (including po*tal cod* and country) « Mascheroder Weg lb, D-3300 Braunschweig, Federal Re¬ public of Germany Date ol depoalt • Acceeaion Number • 23 July 1990 DSM 6126 S. ADDITIONAL INDICATIONS ' (Uate blank If not applicable). 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Claims: CLAIMS 1. Process for treatment of lignocellulosic chemical pulp, wherein the lignocellulosic pulp is treated with an alkaline xylanase at a pH above 7.5, whereafter the thus treated cellulosic pulp is treated with chlorine at an active chlorine multiple of 0.20 or less in the first chlorination stage. 2. Process according to Claim 1, wherein the xylanase treatment is performed at temperatures between 40 and 100°C, preferably between 40 and 80°C, more preferably between 50 and 70°C. 3. Process according to Claim 1 or 2, wherein the xylanase treatment is performed over a period of 15 minutes to 24 hours, preferably between 30 minutes and 5 hours, most preferably between 30 minutes and 3 hours. 4. Process according to Claims 1 - 3, wherein the xylanase treatment takes place at a consistency of 5-35%, preferably 8-25%, most preferably 8-15%. 5. Process according to Claims 1 - 4, wherein the xylanase is producible by means of the microorganisms Malbranchea cinnamomea, Humicola insolens, Bacillus pumilus. Bacillus stearothermophilus. Thermonmonospora fusca or Streptomyces olivochromoqenes. 6. Process according to Claims 1 - 5, wherein an (EOP) treatment is introduced between the xylanase treatment and the chlorine treatment. 7. Process according to Claims 1 - 6, wherein the chlorine multiple is between 0.10 and 0.20.

Inventors: ANKER LISBETH, AUSTIN HELEN F, PEDERSEN LARS SAABY, SKJOLD-JOERGENSEN STEEN, ANKER, LISBETH, AUSTIN, HELEN, F., PEDERSEN, LARS, SAABY, SKJOLD-JOERGENSEN, STEEN
Applicants: NOVONORDISK AS, NOVO NORDISK A/S
Application year: 1990
Main classification: D21C9
IPC codes: C12P19, C12S3, D21C5, D21C9