IMMUNOGENIC GLYCOPROTEINS OF HUMAN CYTOMEGALOVIRUS

Abstract: The invention has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention.

Description: IMMUNOGENIC GLYCOPROTEINS OF HUMAN CYTOMEGALOVIRUS Background of the Inventio-π The number of individual glycαproteins reported to be present in extracellular human cytomega- lovirus (HCMV) has varied. Furthermore, little detailed information has been reported on the inter¬ actions and composition of HCMV glycoproteins. HCMV glycoproteins appear to be involved in human immune recognition of the virus. This has been demonstrated by the reactivity of HCMV glycoproteins with human con- valascent sera. (G. H. Farrar et al., 3. Gen. Virol. , 65, 1991 (19843; B. Nowak et al., Virology, 152, 325 (1984)). Biochemical approaches have been used to try to determine the number and composition HCMV glycopro¬ teins. Farrar et al., cited above, were able to detect five polypeptides in the membranes of HCMV strain AD1 9 which were labeled by carbohydrate-specific mechods. These glycoproteins had molecular weights ranging from 57,000 to 250,000. One method they employed labeled galactose (Gal) and/or N-acetylgalactosamine (GalNAc) residues. With this method, two glycoproteins with molecular weights of 67,000 and 130,000 showed relati- vely high incorporation of label suggesting that these glycoproteins have a distinct phenotype marked by rela¬ tively high concentrations of Gal and/or GalNAc ana possibly high amounts of 0-linked oligosacchariαes. Immunological approaches have also been used to try and identify the number of glycαproteins in HCMV. Several laboratories have generated monoclonal antibodies which recognize one or more disulfide-linked glycoprotein complexes which contain three individual glycoproteins. Several laboratories have reported molecular weights of 130,000, 92-95,000, and 52-55,000 for these glycoproteins (G. Farrar et al., 3. Gen. Virol., 67, 1469 (1986); B. E. Kari et al., 3. Virol., 60, 345 (1986); . M. Law et al., 3. Med. Virol., 17, 255 (1985); L. Rasmussen et al., 3. Virol., 55, 275 (1985)). Kari et al. have disclosed two monoclonal antibodies (41C2 and 9B7) which recognize these glyco¬ proteins. Hereinafter, the parent glycoprotein complex will be referred to as gCI. However, a continuing need exists to identify and characterize HCMV glycoprotein complexes and their constituent glycoproteins ("glyco- peptides" ), particularly those which can elicit a pro¬ tective immune response. Brief Description of the Invention The present invention is directed to composi¬ tions of matter which are substantially-pure glycopro¬ tein complexes or glycoproteins which can be derived from HCMV. These compounds are found only on the membrane envelope of the intact HCMV virion, where the glcyoproteins are associated via disulfide linkages into the glycoprotein complexes. The glycoprotein complexes and their component glycoproteins are physi¬ cally accessible to the immune system and to anti¬ bodies, and are capable of stimulating both humoral and cellular immunity in humans and in other mammals. Therefore, one embodiment of the present invention is directed to substantially-pure, im uno- genic glycoprotein complexes, which are on the membrane envelope of HCMV, wherein said complexes comprise an about 50-52 kD glycαorαtein wnich reacts with the monoclonal antibody produced by hybridoma IVI-10118. Two of these complexes have a molecular weight of about 200 kD or about 93 kD. Hereinafter, these complexes will be referred to as gCII-200 and gCII-93, respec- tively. A further embodiment of the present invention is a substantially-pure, irnmunogenic glycoprotein having a molecular weight of about 50-52 kD, wherein sai'i glycoprotein is on the membrane envelope of HCMV and is associated with other envelope glycoproteins by means of disulfide bonds, and wherein said glycoprotein reacts with the monoclonal antibody IVI-10118. Herεin- aftsr, this complex will be referred to gp52(II). Due to the irnmunogenic properties of these compositions, the present invention also provides a vaccine compris¬ ing an amount of this glycoprotein which is effective to produce an immune response against HCMV in a mammal, when administered thereto, in combination with a pharmaceutically-effective vehicle such as a liquid carrier. For a description of a vaccine employing "glycoσrotein A" of HCMV, see L. Pεreira (U.S. Patent No. 4,689,225), the disclosure of which is incorporated by reference herein. Apart from their use in vaccines, the present compositions are useful in the production of monoclonal antibodies, which in turn can be used to diagnose HCMV or to treat HCMV infections. The present compositions are also useful to produce clonai populations of antigen-specific T-helper lymphocytes, which in turn can be used for HCMV therapy. To prepare the compositions of the present invention, several disulfide-linked glycoprotein com¬ plexes were extracteα from human cytomegalovirus with a παniαnic detergent and seoarated by anion exchange high performance liquid chromatography (HPLC). One co oiex had a molecular weight of 93,000 and was designated as gCII-93. Another related complex had a molecular weight greater than 200,000 and was desig¬ nated as gCII-200. Both complexes were immunoprεcipi- tated with a monoclonal antibody (9E10). A second group of complexes (classified as gCI) immunαprecipi- tated with another monoclonal antibody (41C2). The isolated complexes were reduced, alky- lated, and the individual glycoproteins were separated by gel-filtration HPLC. Glycoproteins with molecular weights of 50-52,000 from gCII-93 and gCII-200 appeared to be the same glycoprotein since they could be immunα- precipitated by 9E10 and had identical peptide maps. The amino sugar content of these glycoproteins was com¬ pared to that of higher molecular weight glycoproteins obtained from gCII-200 and to a glycoprotein with a molecular weight of 93,000 (gp93(I)) from gCI. Glycoproteins with molecular weights of 50-52,000 derived from both gCII-93 and gCII-200 con¬ tained similar amounts of galactαsamine (GalN), glyco- samine (GlcN) and sialic acid. Also, they both con¬ tained 2-3 times more GalN than any other glycoprotein from gCII-200 and 10 times more GalN than was detected in gp93(I). All glycoproteins from gCII-93 or gCII-200 also contained more sialic acid when compared to gp93(I). GalN in these glycoproteins was present in 0- linked oligosaccharides. This was demonstrated by release of low molecular weight oligosaccharides from the high molecular weight glycopeptides by mild base hydrolysis and the conversion of GalN to galactosamin- itol. Thus, gp52(II) appears to have a unique pheno- type marked by a high amount of 0-linked oligosaccha¬ rides. A summary of the various abbreviations employed to refer to these two types of glycoprotein complex, their constituent glycαproteins and the mono¬ clonal antibodies used to immunoprεcipitatε them appears on Table A, below. Table A - HCMV Envelope Glycoprotein Complexes and Glycoproteins Glycoprotein Recognized by Glycoprotein Complex Compoπent(s) Monoclonal (m.w. in kD) (m.w. in kD) Antibodies 41C2 (IVI- gA or gCI GLP-A 1 10119), ~ 9B7 (250-300), (190), (55), (93), (130) (IVI-10117) 5 (160) gB or gCII GLP-B 1 9E10 (93), 3 (200) (50-52) 2 (IVI-10118) S Designation in commonly-assigned U.S. appliction Serial No. 933,789, the entire disclosure of wnich is incorporated by reference herein; the 93 kD gly¬ coprotein is designated gp93(I) herein. gp52(II) herein. gCII-93 herein. gCII-200 herein. In Vitro International. (Linthicum, MD, USA) access code. Detailed Description of the Invention A. Materials and Methods 1. Monoclonal antibodies Production and charac¬ terization of monoclonal antibodies used in these stu¬ dies have been previously described by B. Ξ. Kari et al., 3. Virol., 60, 345 (1986), the disclosure of wnich is incorporated by reference herein. Clonality of monoclonal antibodies 41C2 and 9E10 was established by twice subcloning each antibody. Furthermore, each antibody had an individual isotype (41C2 was a IgGl and 9E10 was a IgG3) and each appears as an individual antibody by isoelectric focusing. ■ o - 2. Purification of HCMV glycoproteins Labeling of Towne strain HCMV with [ 3 H]glucosamine or with [^CJ glucαsamiπe and [ 3 HJ arginine, collection of extra¬ cellular virus, nonionic detergent extraction of gly- ccprotein complexes, and anion-exchange HPLC were done as previously described by B. E. Kari et al., cited above. Complexes designated gCII-93 and gCII-200 were immunoprecipitated with monoclonal antibody 9E10 and complex gC-I was immunoprecipitated with monoclonal antibody 41C2 using protein A sepharose. The immunoprecipitated complexes were solubil- ized by heating them at 60°C for 5 min. in Tris buffer (pH 8) containing 4% SOS. After cooling, urea was added to a final concentration of 8 M and complexes reduced with 10 mM dithiothreitol (DTT) at room tem¬ perature for 4 hrs before adding iodαacetamide. Alky- lation was allowed to proceed for 2 hrs at room temper¬ ature. Samples were dialyzed against 0.1% SDS and reduced in volume to 0.5 ml. Individual glycoproteins were separated by gel-filtration HPLC using coupled columns of TSK 3000 and 4000 (Tαyo Soda, Japan). HPLC was done at a flow rate of 0.3 ml/ in. using a buffer of 10 mM Tris (pH 7.8) containing 0.1% SDS. Eluate was monitored for radioactivity. 3. SDS-PAGE and fluoroαraohv SDS-PAGE was done with 5-15% polyacrylamide slab gel gradients following the method of U. K. Laemmli, Nature, 227, 680 (1970). Tritium in gels was detected by fluorαgraphy using Enlighting (New England Nuclear Corp., Boston, MA). 4. Pronase digestion Pronasε (Caibiochem) diges¬ tion was done at 60°C for 48 hrs as previously des¬ cribed by F. DallOliα et al., J. Virol., 56 , 127 (1985), with the exception that 0.1% SDS was used and enzyme was added twice to a final concentration of 0.3%. Aft3r pronase digestion detergent was removed using Extracti-Gel D (Pierce Chem. Co., Rockford, IL). 5. G-50 column chromatograohy Chromatαgraphy of pronase glycopeptides was done using a Sephadex G-50 column (1X50 cm) equilibrated with 0.1 N acetic acid. The void volume (Vo) was determined with blue dextran and the total included volume (Vt) with [ 3 H] gluco- sa ine. The elution volume of N-linked complex and high mannose glycopeptides was accomplished as pre- viously described by S. Olofsson et al., 3. Gen. Virol., 64, 6735 (1983). 6. Identification of sialic acid Glycopeptides obtained from G-50 chromatography were digested with 0.1 U of neura iπidase (Sigma Chem. Co., St. Louis, M0) in 0.1 M acetate buffer (pH 5) containing 10 M CaCl2 at 37°C for 24 hrs. Samples were applied to a Dowex 1 column in the formate form. The column was washed with 3 column volumes of water to remove unbound label and then with 2 N formate to release siaiic acid. Label released with 2 N formate was further characterized by paper chromatography using n-butanol:water:acetic acid (120:50:30, v/v/v) as the solvent system (solvent A). Radioactive sialic acid standard and label released by 2 N formate was detected by cutting lanes into 1 cm strips and detecting radioactivity by liquid scintilla¬ tion. 7. Strong acid hydrolysis for determination of hexosamines Prior to hydrolysis samples were desalted on P6-DG (Bio Rad). For smaller glycopeptides eluting near the Vt of the G-50 column desalting was done with coupled columns of Dowex 50 (H ) and Dowex 1 (0H ~ ) as previously reported by R. ' G. Spiro, Methods Enzv ol. , 28, 3 (1972). Desalted glycopeptides were hydrolyzεd in 4 N HC1 for 4 hrs at 100°C in a final volume of 1 ml and dried under vacuum. N-acetylgalactosaminitol (GalNAc-αl) was preparεd following the method of W.R.C. Crimmin, 3. Chem. Soc, 2838 (19757). GaiNAc-αl and all other standards were hydrolyzed so that de- acetylated forms were subjected to TLC. 8. Mild alkaline borohydride treatment Alkaline bαrαhydride treatment of pronase glycopeptides was done by the method of R. G. Spiro, cited above. 9. TLC Thin layer chromatography (TLC) was carried out according to the methods of DallOlio et al., cited above, using silica gel 60 on plastic-backed plates from Merck. The solvent system was ethaπol: pyridine:l-butaπol:acetic acid:water (100:10:10:3:30, v/v/v/v/v) to which 1% (w/v) potassium tetrabαrate was added (solvent B). Radioactive hexosamines were detected by fluαrography using Enhance spray (New England Nuclear Corp., Boston, MA) and quaπtitatεd by αensitometric scans of fluorograms. Non-radioactive standards were run in lanes aαjacent to the unknowns. After development of TLC plates, lanes containing stan- dards were cut from the piates and hexosamines dεtected with ninhydrin spray. 10. Peotide mapping HCMV glycoproteins double labeled with L 3 HJ arginine and L 1< CJ glucosamine were digested with insoluble trypsin (Sigma Chem. Co., St. Louis, MO) in Tris buffer (pH 7.8) containing 0.1% SDS for 24 hrs at room temperature with constant mixing. The peptides obtained were separateα by gel-filtration HPLC as described above. 11. DEAE ion-exchange and affinity chromatography DEAE chromatography was done as previously described by F. Serafini-Cεssi et al., Biochem. J., 215, 483 (1983) using a DEAE-sepharose (fast flow, Pharmacia) column (1X10 cm). The elution position of a monosialyiated oligosaccharide was determined using sialyllactose (Sigma Chεm. Co., St. Louis, MO) which was detected by the phenol sulfuric acid assay. Wheat germ agglutitin (WGA) and peanut lectin affinity media were obtained from United States Biochemical Co. Chromatography was done with 1 ml columns. Samples were allowed to adsorb onto the columns for 10 min. before washing with 15 bed volumes of appropriate buffer. Bound material was eluted with the appropriate monosaccharide. B. Results 1. Purification of HCMV complexes and glycopro¬ teins Glycoprotein complexes were extracted from puri¬ fied virus labeled with [ 3 HJGlcN using conditions which are known to solubilize the membrane, but not the nucleocapsid LG. H. Farrar et al., J. Gen. Virol., 65, 1991 (1984)]. As depicted in Fig. 1, fractionation of these extracts by ion-exchange HPLC using a linear gradient of NaCl, generated 4 peaks when glycoproteins were labeled with [ 3 HJGlcN. One peak was not retained by the column. The material in this peak could not be fixed in polyacrylamide gels under conditions which fix proteins, could not be labeled with [ 3S s] methioπine, and was soluble in chloroform methanol (2:1). Based on these observations, it was concluded that this peak contained glycolipid. The three retained peaks depicted in Fig. 1 contained several disulfide-linked glycoprotein com¬ plexes. Peaks 2UR and 4UR contained complexes gCII-93 and gCII-200, respectively, which were recognized by monoclonal antibody 9E10. After reduction of disulfide bonds, gCII-93 generated glycoproteins with molecular weights of 50-52,000, while gCII-200 generated glyco¬ proteins ranging in molecular weight from 50-52,000 to greater than 200,000. Peak 3UR contained gCI com¬ plexes, which were recognized by monoclonal antibody 4IC2. After reduction of disulfide bonds, gCI generated glycoproteins with molecular weights of 130,000, 93,000 and 50,000. Peak 2UR also contained a 93,000 molecular weight glycoprotein which was not disulfide-linkεd to other glycoproteins and was not immunoprecipitated by the monoclonal antibodies. This glycoprotein was not examined further. Glycoprotein complexes gCII-93 and gCII-200 were immunoprecipitated with 9E10 and gCI was im uno- precipitated with 41C2. Immunoprecipitated complexes were solubilized in SDS, reduced with DTT, alkylated with iodoactεa ide, and the individual glycoprctεins were separated by gel-filtration HPLC. By gel-filtra¬ tion, we were able to obtain a 93,000 molecular weight glycoprotein Cdesignated gp93 (I)) from gCI (Fig. 2A). Material from gCII-93 was resolved into two peaks (Fig. 2B). A minor peak contained a 90,000 mole¬ cular weight glycoprotein, while a second major peak had a glycoprotein with a molecular weight of 50-52,000 (designated gp 52 (11-93)). Due to the small amount of the 90,000 molecular weight glycoprotein, only gp52 (11-93) was collected for further analysis. After reduction of disulfide bonds, gCII-200 resolved into at least 3 peaks (Fig. 2C). The first peak contained material with a molecular weight greater than 200,000 (designated gp200 (11-200)); the second peak contained a smear of material ranging in molecular weight from 90,000 to 200,000 (designated gp90 (11-200)) (Fig. 2C). The third peak was the most abun- dant when glycoproteins were labelεd with L 3 HjGlcN. This peak containing glycoproteins with molecular weights of 50-52,000 (designated gp52 (11-200)) (Fig. 2C). 2. Immunoprscioitation and oeotide mapping of qp52 ClI-95) and gp52 (11-200) The glycoproteins in glyco¬ protein complexes gCII-93 and gCII-200 are immunopreci¬ pitated by 9E10 after reduction of disulfide bonds and appear to be immunologically-related. The relationship between these glycoproteins, gp52 (11-93) and gp52 (11-200), was examined by re-immunoprecipitating each as a purified glycoprotein with 9E10 and by peptide mapping. Both purified glycoproteins could be immuno¬ precipitated by 9E10, further establishing their im u- nological relationship. For peptide mapping, glycopro¬ teins were double labeled with [ 3 H3argiπine and [ 1 c]- GlcN. Glycoprotein 93 (I) was examined for comparison. The purified glycoproteins were digested with trypsin and fragments separated by gel-filtration HPLC. By this method, three major pεptides werε obtained from gp93 (I). All three contained carbohydrate, suggesting that gp93 (I) contains at least three glycosylation sites. With gp52 (11-93) and gp52 (11-200), two major fragments were obtained. One eluted with a molecular weight of approximately 30,000 and contained most of the L^CJGlcN. A second fragment had a molecular weight of approximately 10,000 ana contained little or no [ 11 *C]G1CN. Smaller, less abundant peaks were also detected. With both glycoproteins, the most abundant of these eluted with arginine. 3. Analysis of HCMV glycoproteins for amino sugar content The purified glycoproteins from Towne strain HCMV were analyzed to determine their amino sugar con¬ tent. These studies were done with [ 3 K3G1CN labeled glycoproteins. When [ 3 HjGlcN is used, protein-bound radioactivity can be found in GlcN, GalN and sialic acid, allowing detection and quantitation of these amino sugars. Labeled glycoproteins were digested with pronase and glycopeptides were separated by G-50 chro¬ matography. The separated glycopeptides were subjected to neuraminidase digestion prior to strong acid hydro- lysis to quantitatε the amount of sialic acid present. Radioactivity released by neuraminidase was bound to a Dowεx 1 column in the formate form and was rεiεasεd by 2 N formate. In addition, labεl eluting with 2 N for¬ mate co-migrated with authentic sialic acid with paper chromatography using solvent system A. The amount of radioactivity released by neuraminidase was used to quaπtitate the amount of sialic acid in the various glycoproteins. To quantitatε the amount of GlcN and GalN, desialylated glycopeptides were subjected to strong acid hydrolysis and hexosamines separated by TLC. Separation of pronase glycopeptides from gp93 (I) by G-50 chromatography generated a minor peak which eluted near the Vo. This peak contained GalN and GlcN. However, with gp93 (I), most of the glycopeptides werε found in the partially included volume, eluting with N- linkεd glycopeptide standards. Ninety eight percent of the radioactivity in this peak was incorporated as GlcN. Quantitation of radioactivity in all amino sugars in gp93 (I) showed that 86% of the radioactivity was in the form of GlcN (Table B). Table B Distribution of Radioactivity in Sialic Acid, GlcN and GalN Glycoprotein Sialic Acid (a) GlcN (b) GalN (%) gp93 (I) 9 gp200 (11-200) 13 gp90 (11-200) 14 gp52 (11-200) 15 gp52 (11-93) 14 (a) Each G-50 peak was treated with neuraminidase and the reaction mixture applied to a Dowex 1 column in the formate form. The radioactivity which bound to the column was eluted with 2 N formate. This amount of radioactivity was divided by the total amount of radioactivity applied to obtain the percentage of radioactivity in the form of sialic acid. (b) The material from each G-50 peak which did not bind to the formate column was subjected to acid hydrolysis to obtain individual hexosamines. Hexosamines were separated by TLC and detected by fluσrography. The relative amount of radioactiv¬ ity in the form of GlcN and GalN was determined by scanning the fluorograms. These values were multiplied by the fractional amount of neutral radioactivity in each G-50 peak. The data summarized on Table B suggests the gp93 (I) contains largely N-linked oligosaccharides. After pronase digest, glycopeptides from gp52 (11-93) and gp52 (11-200) were resolved into three peaks by G-50 chromatography. The first peak eluted at the Vo. Material in this peak was collected and redi- gested with pronase for 24 hrs with no further degrada¬ tion. The glycopeptides eluting at the Vo may have contained closely spaced oligosaccharides on the pep¬ tide backbone which can prevent the action of pronase. While G-50 peak I from both gp52 (11-93) and gp52 (11-200) contained 72-75% GalN, the peak itself was more abundant in gp52 (11-200). The second peak (G-50 peak 2) eluted with N-linked standards and contained 82-91% GlcN, indicating the presεnce of N-linked oligo¬ saccharides in these glycoproteins. The third peak (G-50 peak 3) eluted near the Vt and contained similar amounts of GlcN and GalN. Both gp90 (11-200) and gp200 (11-200) were examined in the same manner as gp52 (11-200). The results from these expεriments were used to quantitate the relative amount of radioactivity incorporated in the different amino sugars (Table B). The relative amount of radioactivity found in the form of sialic acid in glycoproteins from gCII-93 and gCII-200 was very similar, but significantly greater than that detected in gp93 (I) (Table B). Moreover, all glyco¬ proteins from gCII-93 and gCII-200 contained more GalN than gp93 (I). However, gp52 from both gCII complexes contained two to three times more GalN than αthεr gly- coprotεins from the same complexes. 4. Analysis of glycopeptides obtained after mild alkaline borohvdride treatment Since glycoproteins from gCII-93 and gCII-200 contained high amounts of GalN, it was of interest to determine if the GalN was present in 0-linked oligosaccharides. To determine this, the glycopeptides were subjected to the beta- εiiminatiαn reaction which cleaves oligosaccharides which are O-glycosidically-linked to serine or threαnine. N-acetylgalactosamiπe, which is usually present at the reducing end of the oligosaccharide, is converted to GalNAc-ol. Oligosaccharides obtained after beta-elimination were subjected to strong acid hydrolysis and hexosamines examined to demonstrate the conversion of GalN to GalN-αl. When glycopeptides in the G-50 peak 1 of gC-II glycαproteins were re-chromatographed on G-50 after beta-elimination, there was a shift from the Vo to near the Vt. The molecular weight of this material was approximately 1500. This result was obtained with all glycoproteins from gCII-93 or gCII-200. There was also material which eluted earlier as a small peak or shoulder. Material eluting early contained GlcN, indi- eating it contained N-linkεd oligosaccharides, while material eluting near the Vt co-migrated with GalN-ol. A small amount of radioactivity in the Vt peak migrated in front of the GlcN standard. This fast migrating material was most likely degradation products obtained by strong acid hydrolysis of GalN-αl. The elutiαπ position of G-50 peak 2 was not affected by the beta- elimiπatiαn reaction carried out on any glycoprotein from gCI, gCII-93 and gCII-200. This peak contained GlcN, demonstrating that the oligosaccharides were N- linked. The elution position of G-50 peak 3 from any glycoprptein from gCII-93 or gCII-200 was not affected by beta-elimination, but GalN in this peak was con¬ verted to GalN-ol. This suggested that the GalN in this peak was O-glysosidically linked. 5. Degree of sialylation and terminal sugar resi- dues of 0-linked oligosaccharides The oligosaccharides from gp52 (11-93) and gp52 (11-200) were examined to measure their degree of sialylation. These studies were confined to glycopeptides from G-50 peak 1 since it contained most of the 0-linked oligosaccharides. For these studies, gCII-93 and gCII-200 were immunαprε- cipitatεd dirεctly from dεtergent εxtracts without prior separation by ion-exchange chromatography. By this method, gp52 (11-93) and gp52 (11-200) were iso- latεd as one glycoprotein designated gp52 (II). By DEAE chromatography, glycopeptides in G-50 peak 1 were resolved into three peaks. Of the applied radioactivity, 17% was not rεtainεd by the column. As determined by TLC, this peak contained only GalN, suggesting that some 0-linked oligosaccharides were neutral and did not contain sialic acid. Peak 2 eluted at a salt concentration needed to elute a monαsialy- latεd αligosaccharide and contained 96% GalN. This suggested the presence of 0-linked oligosaccharides containing a single sialic acid residue. Most of tne GlcN in G-50 peak 1 was detected in peaks 3 and 4, but these peaks still contained 85% GalN, suggesting that some 0-linked oligosaccharides contained more than one sialic acid residue. The uneven distribution of hexo- samines in the DEAE peaks suggested that glycopeptides containing N- and 0-linked oligosaccharides were bind¬ ing independently or that there were differεnt glyco¬ peptides, some with only 0-linked oligosaccharides and some with N- and 0-linkεd oligosaccharides. To determine the nature of the non-reducing terminal monosaccharides in glycopeptides from G-50 peak 1, a series of lectin affinity columns were usεd. Most of the glycopeptides from G-50 peak 1 were retained by the DEAE column, indicating that they con- tained sialic acid. Therefore, glycopeptides were applied to a WGA column which has affinity for non- reducing terminal sialic acid and GlcNAc residues [K. Ya amotα et al., Biochemistry, 20, 5894 (1981)] . When this was done, 85% of the radioactivity bound to the column. Taken together, DEAE and WGA affinity chroma¬ tography suggest that these glycopeptides contain non- reducing terminal sialic acid residues. The portion which did not bind WGA would con¬ tain the neutral glycopeptides detected by DEAE chroma¬ tography. These glycopeptides were applied to a peanut lectin column which has strong affinity for the struc- ture Gal-GalNAc [i. 3. Goldstein et al., Advances in Carbohydrate Chemistry and Biochemistry, Academic Press, NY, 35, 127-340 (1978)]. Of the radioactivity applied, 58% bound and was eluted with 0.05 M Gal, indicating that 0-linked oligosaccharides werε present which had Gal as a terminal residue. Furthermore, since sialic acid is often the terminal sugar linked to the penultimate residue Gal of both N- and 0-liπked oligosaccharides, it was of interest to treat that por¬ tion which originally bound to WGA with neuriminidase to remove sialic acid. Prior to neuraminidase treat¬ ment, less than 10% of the radioactivity which bound WGA had affinity for peanut lectin. After neuramini¬ dase treatment, 81% of the radioactivity bound to the column and was eluted with Gal. This result is coπ- sistent with the interpretation that oligosaccharides in this fraction have a terminal sialic acid residue and a penultimate Gal residue. C. Discussion While there have been a number of reports characterizing gC-I and its glycoproteins, there have been no reports other than Kari et al. , cited above, describing gC-II and its glycoproteins. Based upon the studies reported hereinabove, the 50-52,000 molecular weight glycoproteins in gCII-93 and gCII-200 appear to be the same glycoprotein. This conclusion is based on several observations. First, they have the same molecular weight in SDS-PAGE and are immunoprecipitated by the same ono- clαnal antibody (9E10). They also had identical pep¬ tide maps and the amino sugar content of proπasε glyco¬ peptides was similar. The major difference between gp52 (11-93) and gp52 (11-200) appears to be their association in different disulfide-linked complexes. Glycoproteins present in multimeric forms in viral membranes is not unprecεdεnted. For example, glycopro- tein C has been detected in multimeric forms in HSV-2. Of the two glycoprotein complexes which we detεcted, gCII-200 appears to be more abundant based on ion- exchange HPLC data. Therefore, glycoproteins gp52 (11-93) and gp52 (11-200) were collectively designated gp52 (II). All of the HCMV Towne strain glycoproteins which we isolated contained N-linked oligosaccharides. These were identified as N-linked by sevεral critεria. First, they co-eluted with N-linked standards on gel- filtration and contained high amounts of GlcN. More¬ over, GlcN was still detected after mild alkaline treatmεnt and thεir elutiαn position did not change. The N-linked oligosaccharides were not examined further. Very few viral glycoproteins have beεπ charac- tεrized which contain high amounts of 0-linked oligo¬ saccharides. Of the HCMV glycoproteins characterized, 0-linked oligosaccharides were most abundant in gp52 (II). It appears that gp52 (II) contains a cluster of hydroxyamino acids to which some of the 0-linked oligo¬ saccharides are bound. This is basεd on the observa¬ tion that pronase resistant high molecular weight glycopeptides containing high amounts of GalN were always obtained. The nearly co plεte conversion of the GalN in this fragment to GalN-ol indicated that most of the GalN was at the reducing end of the oligosaccharide attached to either serine or theronine. Thus, this fragment would have to contain a number of hydroxamino acids. The 0-linkεd oligosaccharides of gp52 (II) appear to be terminated by at least Gal and sialic acid. Neutral 0-linkεd oligosaccharides were detected by DEAE chromatography. Among the neutral oligo¬ saccharides were those apparently terminated by Gal as determined by their affinity for peanut lectin. Hαw- ever, of the glycopeptides tested, most bound DEAE and required pretrεatment with nεuramiπidase to bind peanut lectin. These results suggested that some 0-liπked oligosaccharides were terminated with Gal, but in others, sialic acid was the terminal residue and Gal the penultimate. It is also possible, based on DEAE data, that some of the 0-linked oligosaccharides con¬ tained more than 1 sialic acid residue. Our results also show that gp52 (II) contains a high content of Gal and GalNAc. Farrar and Oram, 3 __ Gen. Virol., 65 , 1991 (1984), also found glycoproteins in HCMV strain AD169 which had high amounts of Gal and/or GalNAc. However, the lowest molecular weight they reported for a glycoprotein with these character¬ istics was 67,000. We found the molecular weights of the 0-linked oligosaccharides to be approximately 1500 after beta- elimination. However, the presence of sialic acid in an oligαsaccharide can increase its apparent molecular weight in gel-filtration. Thus, the molecular weights of these oligosaccharides may actually be less. Glyco¬ proteins which normally contain 0-linked oligo¬ saccharides in this molecular weight range include the proteoglycans, immunoglobulin light chains and MN blood group glycoprotein. These oligosaccharides contain GalNAc, Gal, and 1 to 2 sialic acid residues. Sialic acid in most of these oligosaccharides is the non- reducing terminal monosaccharide while Gal is the penultimate. Thus, it seems that the enzymes involved in the synthesis of these oligosaccharides must be active in cells infected by HCMV. The Invention has been described with refer¬ ence to various specific and prεfεrrεd εmbodiments and techniques. However, it should be understood that many variations and modifications may be made while rεmain- ing within the spirit and scope of the invention.

Claims: WHAT IS CLAIMED IS: 1. A substantially-pure, irnmunogenic glycoprotein complex which is derivable from the membrane envelop of human cytomegalovirus, has a molecular weight of about 200kD or about 93kD, contains at least one disulfide bond, is immunoreactable with the monoclonal antibody produced by hybridoma IVI-10118, and has a glycoprotein subunit of molecular weight of about 50 to 52k which is produced by disulfide reduction. 2. A complex according to claim 1 wherein the subunit glycoprotein contains at least about two glycosylation sites and at least the amino-sugars galactosamine, glycosamine and sialic acid. 3. A complex according to claim 2 wherein the maj-ority of amino-sugar present comprises a combination of galactosamine. and glycosamine. 4. A complex according to claim 2 wherein 0-linked oligosaccharides are present. 5. A complex according to claim 4 wherein the 0- linked oligosaccharides comprise galactosamine. 6. A substantially pure irnmunogenic glycoprotein which has a molecular weight of about 50 to 52 kD, has at least one sulfhydryl group,, is immunoreactable with the monoclonal antibody produced by the hybridoma IVI-10118, is derivable from the membrane envelop of human cytomegalovirus, and when within said envelop, the glycoprotein is associated with other envelop components bv means of disulfide bonds. 7. A glycoprotein according to claim 6, containing at least about two glycosylation sites and at least the amino—sugars galactosamine, glucosamine and sialic acid. 8. A glycoprotein according to claim 7 wherein the majority of amino-sugar present comprises a combination of galactosamine and glycosamine. 9. A glycoprotein according to claim 7 wherein O- linked oligosaccharides are present. 10. A glycoprotein according to claim 9 wherein the O-linked oligosaccharides comprise galactosamine. 11. A vaccine comprising an amount of the glycoprotein of any of claims 6 through 10, which effectively produces an immune response against human cytomegalovirus in a mammal, in combination with a pharmaceutically acceptable carrier.

Inventors: GEHRZ RICHARD C, KARI BRUCE E, GEHRZ, RICHARD, C., KARI, BRUCE, E.
Applicants: CHILDRENS HOSPITAL INC, THE CHILDREN'S HOSPITAL, INCORPORATED
Application year: 1989
Main classification: C07K15
IPC codes: A61K39, C07K1, C07K14, C07K16, C12P21, C12R1, G01N33