Improved chemical process for preparing antibiotic L 17392 (deglucoteicoplanin) and its salts

Abstract: The present invention is directed to a chemical process for preparing antibiotic L 17392 (deglucoteicoplanin) and its salts with bases and acids by submitting a teicoplanin compound or a teicoplanin-like compound to controlled strong acid hydrolysis in the presence of an organic aprotic solvent, and a purification thereof.

Description: The present invention is directed to the obtainment of an antibiotic substance arbitrarily designated as antibiotic L 17392 or deglucoteicoplanin and its salts with bases and acids. The antibiotic substances possess antimicrobial activity mainly against gram-positive bacteria (e.g. Staphylococcus and Streptococcus strains). This antibiotic is obtained by chemical transformation of a teicoplanin compound or teicoplanin-like compound. Teicoplanin is the international non-proprietary name (INN) of the antibiotic substance formerly named teichomycin which is obtained by cultivating the strains Actinoplanes teichomyceticus nov. sp. ATCC 31121 in a culture medium containing assimilable sources of carbon, nitrogen and inorganic salts (see U.S. Patent No. 4,239,751). According to the procedure described in the above cited patent, an antibiotic complex (identified as teichomycin) containing factors A₁, A₂ and A₃ is recovered from the fermentation broth by extraction with a suitable water insoluble organic solvent and precipitation from the organic solvent according to common procedures. Factor A₂, which is the preponderant factor of the isolated antibiotic complex, is then separated from the other factors by means of column chromatography on Sephadex R . Factor A₁ and factor A₃ are present only in minor amounts. British Patent No. 2121401 discloses that antibiotic factor A₂, in turn, actually is a mixture of five closely related co-produced main components. From a fermentation and purification (for instance, through column chromatography) operations a teicoplanin product is currently obtained which essentially consists of factor A₂ accompanied by minor amounts of factor A₃. Recent studies shows that teicoplanin factor A₂ and its individual components may be represented by the following formula I wherein R is a N-[(C₉-C₁₂ aliphatic acyl]-D-glucosamine rest, R¹ is a N-acetyl-D-glucosamine rest, and R² is a D-mannose rest. All sugar moieties identified above are linked to the core/molecule through O-glycosidic bonds. A substance having the same structural formula is disclosed in EPA Publication No. 0090578 and is named antibiotic A 41030 factor B. This substance is obtained by means of a microbiological process which involves the fermentation of the strain Streptomyces virginiae NRRL 12525 or Streptomyces virginiae NRRL 15156 in a suitable medium, the isolation, purification and separation into its components of antibiotic A 41030, an antibiotic complex of at least seven factors, antibiotic A 41030 factor B, included. In the European Patents No. 119574 and 119575 have been described partial hydrolysis products of teicoplanin factor A₂ wherein one or two sugar moieties are split off. These products are respectively named antibiotic L 17054 and L 17046. The products are obtained by submitting teicoplanin factor A₂ to some specific acid hydrolysis conditions. For L 17054 the hydrolysis is preferably carried out by using 0.5 N hydrochloric acid at a temperature between 70°C and 90°C for 15 to 90 minutes. For L 17046, the hydrolysis is preferably carried out by using hydrochloric acid at a concentration from 1 N to 3 N at a temperature between 70°C and 90°C for 30 to 60 minutes. Antibiotic L 17054 may be represented by the formula I above, whereby R is replaced by hydrogen, R¹ is a N-acetyl-D-glucosamine rest and R² is a D-mannose rest. Antibiotic L 17046 may be represented by the formula I above wherein R and R² are both replaced by hydrogen and R¹ is a N-acetyl-D-glucosamine rest. European Patent Application Publication No. 301247 describes the de-mannosyl teicoplanin derivatives, i.e. compounds of the formula I above wherein R and R¹ are as above and R² is hydrogen. In this specification and claims, with the term "teicoplanin compound" it is indicated a substance selected from the teicoplanin complex obtained by fermentation of Actinoplanes teichomyceticus ATCC 31121 followed by purification operations according to US Patent No. 4,239,751, any further purified preparation thereof, teicoplanin factor A₂, teicoplanin factor A₃, each of the main components of teicoplanin factor A₂. The term "teicoplanin compounds" includes also teicoplanin RS-1 and RS-2 described in the paper given by M. Zanol et al, at the 17th International Symposium on Chromatography, Vienna, September 25-30, 1988 and teicoplanin RS-3 (compound A) and RS-4 (compound B) described in European Patent Application Publication No. 306645. These compounds may be represented through the formula I above wherein R¹ and R² are as above and the aliphatic acyl moiety of the radical R are respectively 10-methyl-undecanoyl, dodecanoyl, 6-methyl-octanoyl and nonanoyl. With the term "teicoplanin-like compound" it is hereby indicated any compound having the same basic structure formula I as above wherein R is hydrogen or a N-[(C₉-C₁₂)aliphatic acyl]-D-glucosamine rest, R¹ is hydrogen or a N-acetyl-D-glucosamine rest, R² is hydrogen or a D-mannose rest with the proviso that R, R¹ and R² cannot be simultaneously hydrogen, and a mixture of two or more of any of the above substances and/or compounds in any proportion. European Patent Application Publication No. 146053 describes a chemical process for preparing antibiotic L 17392 (deglucoteicoplanin i.e. the compound of formula I wherein R, R¹ and R² are simultaneously hydrogen atoms) by submitting a teicoplanin compound or a teicoplanin like compound to controlled strong acid hydrolysis characterized in that an organic protic solvent selected from aliphatic acids, alpha halogenated aliphatic acids, aliphatic and cycloaliphatic alkanols, and phenyl substituted alkanols, is used. According to the disclosure of European Patent Application Publication No. 146053 it is also necessary to employ a strong acid compatible with the solvent such as a strong mineral acid or a strong organic acid, and carry out the reaction at a temperature between about 20°C and about 100°C. In the "Journal of Antibiotics" Vol. 39, No. 10 pp. 1430-1442 October 1986 the preparation and the NMR characteristics of deglucoteicoplanin are described. In said reference different methods for preparing deglucoteicoplanin, by hydrolysis in an organic polar solvent of a teicoplanin and teicoplanine like compounds are reported. According to an object of this invention it has been found that antibiotic L 17392 (deglucoteicoplanin) and its salts with bases and acids may be obtained by submitting to controlled strong acidic hydrolysis conditions a teicoplanin compound or a teicoplanin-like compound in a polar aprotic organic solvent selected from N,N-dimethylformamide (DMF), hexamethyl phosphoramide (HMPA), 1,3-dimethyl 3,4,5,6-tetrahydro-2 (1H) pyrimidone (DMPU) and, dimethyl sulfoxide (DMSO) or mixture thereof. The "controlled strong acidic hydrolysis conditions" which are suitable for the process of this invention are those reaction conditions whereby it is provided sufficient acid strenght to provoke the removal of all sugar moieties of teicoplanin compounds and/or teicoplanin-like compounds without simultaneously provoking other undesired modifications or alteration of the chemical structure and chiral centers of the substrate. It is known that removal of all sugar moieties from a complex molecular structure such as that of glycopeptide antibiotics always presents considerable difficulties since mild acid hydrolysis conditions usually afford only partial removal of sugars moieties while strong acid hydrolysis conditions promote partial degradation of the substrate and/or changes in the stereochemical configuration of chiral centers. For instance, for avoparcin, a known glycopeptide antibiotic, the true aglycone was never isolated. The following scientific literature support the above considerations: G.A. Ellestad et al., J. of Antibiotics, 36, 1683 (1983); C.M. Harris et al., J. Am. Chem. Soc. 105 , 6915 (1983); W.J. McGahren et al., J. of Antibiotics, 36 , 1671 (1983). M.R. Bardone et al., (J. of Antibiotics, 31 , 170 (1978)) describes hydrolytic treatments of teichomycin factor A₂ both with aqueous 2 N H₂SO₄ and with aqueous 6 N HCl at 100°C. It has been found that with the process of the present invention the molar yield of deglucoteicoplanin is remarkably high (about 65%). The yields of deglucoteicoplanin of the processes of the prior-art depend on the particular combination solvent/reactant used. For example by using trifluoroacetic acid as solvent and HCl as acid medium it is possible to have yields comparable to those of the present invention, while by employing H₂SO₄ the yields of the prior-art processes are lower than 10%. Alternative methods disclosed in the prior-art which apparently give very high yields (about 90% of the crude product), have the drawbacks of being necessarily carried out in a heterogenous medium (suspension of the starting material in a liquid polar solvent, bubbling the mineral acid) and actually afford a crude product which has a very low grade of purity. For instance in Example 4 of European Patent Application Publication No. 146053 starting from a suspension of ten grams of teicoplanin complex in benzyl alcohol, using HCl as mineral acid at a temperature of 60°C, 9.2 g of crude deglucoteicoplanin are obtained. However after chromatographic purification only 1.5 g of substantially pure product are recovered. Furthermore, in view of a scaling up of the process it is important to carry out the reaction in a homogeneous medium in order to have a better reproducibility of the quality of the product, such as a better homogenization of the granulometry and less analytical problems during the reaction course. With the process of the invention it is possible to have good yields of deglucoteicoplanin having a good grade of purity and to carry out the reaction in a homogeneous medium. According to the object of this invention the "controlled strong acid hydrolysis conditions" outlined above are provided by properly selecting the solvent, the type of acid, its concentration, and the temperature of the reaction. In fact, it is hereby provided a process for transforming a teicoplanin compound or a teicoplanin-like compound into deglucoteicoplanin and its salts with bases and acids, characterized in that a substance selected from teicoplanin complex, any further purified preparation thereof, teicoplanin factor A₂, teicoplanin factor A₃, each of the main components of teicoplanin factor A₂, a compound of formula I above wherein R is hydrogen, or a N-[(C₉-C₁₂)aliphatic acyl]-D-glucosamine rest, R¹ is hydrogen or a N-acetyl-D-glucosamine rest, R² is hydrogen or a D-mannose rest with the proviso that R, R¹ and R² cannot be simultaneously hydrogen, and a mixture of two or more of any of the above substances in any proportion, is submitted to controlled strong acid hydrolysis conditions in a homogenous medium by employing an organic aprotic solvent selected from N,N-dimethylformamide (DMF), hexamethyl phosphoramide (HMPA), 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H) pyrimidone (DMPU) and dimethylsulfoxide (DMSO) a mixture thereof, and a strong acid compatible with the solvent selected from a strong mineral acid and a strong organic acid. The organic aprotic solvent has to be liquid at the reaction temperature and has to be capable of completely solubilizing the teicoplanin starting material. The organic aprotic solvent which is particularly preferred is dimethylsulfoxide. The strong acid which is needed to provide the controlled strong acid hydrolysis conditions of the process of the invention can be a strong mineral acid or a strong organic acid. Among the strong mineral acids hydrochloric acid, hydrobromic acid, concentrated sulfuric acid and concentrated phosphoric acid are preferred. Among the strong organic acids the alpha-halogenated lower aliphatic acids, the alkanesulfonic acids, the polyfluoroalkanesulfonic acids, the cycloalkanesulfonic acids and the arylsulfonic acids are preferred, with the following being the most preferred ones: trifluoroacetic acid, trichloroacetic acid, methanesulfonic acid, ethanesulfonic acid, trifluoromethanesulfonic acid, benzenesulfonic acid, cyclohexanesulfonic acid, camphorsulfonic acid, alpha and beta naphtalene sulfonic acid and p-toluenesulfonic acid. The concentrated acids are preferably employed in large excess (by weight) on the starting teicoplanin or teicoplanin-like compound. Sulfuric acid and phosphoric acid are usually employed at the highest concentration commercially available. In particular, 95% to 98% (w/w) sulfuric acid and 85% to 98% (w/w) orthophosphoric acid yield satisfactory results. However, the process can be carried out also by any strong mineral acid at lower concentration, such as for instance 30% to 80%. Among the organic acids, 98% (w/w) methanesulfonic acid and 98% (w/w) trifluoroacetic acid are preferably employed according to a preferred embodiment of this invention. The content of water in the reaction mixture must be sufficient to meet the stoichiometric requirement of the hydrolysis reaction. On the other side, it is desirable to keep the amount of water in the reaction mixture as low as possible to prevent unwanted side reactions such as degradation of the reaction product, or rearrangement and/or isomerization of the chiral centers of the substrate. For meeting the stoichiometric requirements of the reaction and, at the same time, keeping the rate and the process operability at an industrially valuable level, the hydrolysis process is usually carried out in the presence of an amount of water which is ranging from about 1% to about 20% by weight of the starting teicoplanin or teicoplanin-like compound. The sufficient water amount may be supplied together with the acid or it may be originally contained in the starting substrate. When essentially anhydrous starting material, solvent and acid are employed, the desired amount of water may be directly added to the solvent or to the reaction mixture. When a mineral acid is used, the total concentration of the acid in the reaction mixture depends on the particular acid used but usually ranges between 1% (w/v) and 10% (w/v). For example when H₂SO₄ is used the concentration may range between 2% and 7% (w/v), more preferably between 3% and 5%, while when HCl is employed it is preferred to use a lower concentration, for example comprised between 1% and 3% (w/v). As a general illustration of the controlled strong acid hydrolysis process, the teicoplanin or teicoplanin-like compound is stirred in a molar excess of the selected solvent at room temperature and then the proper acid is added. The reaction mixture is then reacted and maintained with stirring at the desired temperature for a period of time enough to achieve satisfactory yields of deglucoteicoplanin. The reaction time is usually determined by monitoring the reaction through analytical tests. For the purpose of giving a general indication without limiting the scope of this invention, the reaction time may generally range between 0.25 and 20 hours, depending on the starting material, the solvent(s), the strong acid, its concentration and the temperature of the reaction. The temperature of the reaction is properly selected for each reaction system, taking into accounts the following factors: the starting materials, the type of the organic aprotic solvent, the type and the concentration of the acid, and their mutual proportions. Lower reaction temperature usually requires a longer reaction time for obtaining satisfactory conversion yields. In general the controlled strong acid hydrolysis process is carried out at a temperature between about 50°C and about 120°C. A preferred temperature range is between 60°C and 100°C with the range between 75°C and 85°C being the most preferred one. At the end the reaction mixture is poured into demineralized water under stirring and the pH is adjusted to about 7.0 with a basic aqueous solution such as for example 10% aqueous NaOH. The reaction mixture is then filtered. The solid obtained by filtration is washed with a small amount of water and dried at 50°C under vacuum for two hours. In a preferred embodiment of the invention DMSO is used as solvent in the presence of 98% (w/w) H₂SO₄ at a temperature of about 85°C. The concentration of the sulfonic acid in the total volume of the reaction mixture is generally comprensive between 3% and 5%. Generally, after hydrolysis the solid crude product has a purity of about 30-35%. In a further aspect of the invention, the crude deglucoteicoplanin obtained as described above can be easily purified by simple acid-base precipitation from a DMSO/water mixture, thus avoiding any further purification step. In fact it is known that by using the hydrolysis methods of the prior-art it is necessary to purify the deglucoteicoplanin by column chromatography for example, by use of a reverse phase column chromatography employing silanized silica gel as adsorbent and hydrophilic mixtures as eluent. Obviously, any purification procedure known in the art can be used for purifying the crude deglucoteicoplanin obtained through the process of the invention, although a purification procedure which comprises a chromatographic method has the disadvantage of requiring elution times and amounts of solvent which are not adequated to the scaling up of the process. Therefore a further object of the invention is a process for purifying deglucoteicoplanin which comprises dissolving said deglucoteicoplanin in a suitable solvent mixture containing DMSO/H₂O at a pH value lower than about 4 or higher than about 10, reprecipitating the product adjusting the pH of the solution between 6 and 8, preferably at 7, and filtering the resulting pure solid. The pH value of the solution is brought to a value lower than 4 or higher than 10 in order to solubilize deglucoteicoplanin as an acid or base salt in a reasonable small volume of the solution thus lowering the loss of the final product when it is precipitated. For example addition of strong mineral acids such as HCl, HBr or H₂SO₄ can be preferably used in order to obtain the acid salt form. An alkali hydroxide such as NaOH is preferably used when is desired to dissolve deglucoteicoplanin in a base salt form. The DMSO/H₂O solvent mixture has to show a good dissolution power toward the deglucoteicoplanin starting material. Usually the DMSO concentration in the DMSO/water mixture ranges between 30% and 70% (v/v) preferably between 40% and 60%. With 10 to 20 weight proportions of mixture of this latter range it is generally possible to dissolve one weight proportion of crude deglucoteicoplanin at the above mentioned acid or basic pH value. In a preferred embodiment of this particular aspect of the invention a DMSO/water mixture containing 40-60% DMSO/water in the presence of 10% aqueous hydrochloric acid sufficient to bring the pH value at about 4 is used to dissolve the crude deglucoteicoplanin (30-35% HPLC titre). The deglucoteicoplanin obtained by precipitation from the solution through adjustement of the pH at a neutral value comprised between 6 and 8, preferably at 7 is a product in form of free base, which does not require any further purification step. In case that the crude deglucoteicoplanin has a purity grade lower than 30% it may be necessary to repeat the solution/precipitation step. A final double sludge of the solid, first with acetone and then with demineralized water can be also introduced into this procedure in order to eliminate the residual DMSO. The thus obtained product is substantially pure deglucoteicoplanin showing satisfactory physico-chemical and biological characteristics for use in the applications described below. Substantially pure deglucoteicoplanin has an HPLC titre greater than 95% (percent peak areas, at the 254 nm U.V. wavelenght), a water and solvent content from 10% to 15% by weight and an inorganic residue lower than 0.5% by weight. The physico-chemical characteristics of the deglucoteicoplanin as obtained with the process of the invention are reported in the European Patent Application Publication No. 146053 already cited. Deglucoteicoplanin obtained according to this process can be optionally transformed into the corresponding salts with bases and acids through commonly known procedures. Deglucoteicoplanin and its pharmaceutically acceptable salts with bases and acids can effectively be employed as the active ingredient of antimicrobial preparations used in human and veterinary medicine for the prevention and treatment of infectious diseases caused by pathogenic bacteria which are susceptible to said active ingredients. In such treatments, these compounds may be employed as such or also in the form of mixtures in any proportion. The data concerning in vitro and in vivo activity of deglucoteicoplanin, as well as the way of formulating it are disclosed in European Patent Application Publication No. 146053. However, deglucoteicoplanin can be used as the starting material for the preparation of valuable semisinthetic teicoplanin derivatives such as those described in European Patent Application Publication No. 218099 and International Patent Application Publication No. WO 88/06600. The following examples illustrate the manner in which the invention can be practiced, but, as such, should not be construed as limiting its overall scope. Example 1 - Preparation of deglucoteicoplanin from teicoplanin complex 4.5 g of Teicoplanin complex sodium salt (i.e.sodium salt of the antibiotic complex containing teichomycin factors A₁, A₂ and A₃ and obtained by fermentation of strain ATCC 31121 according to US Patent No. 4,239,751) were suspended in about 42 ml of DMSO at room temperature (20°C) and 1 ml of 95% H₂SO₄ (corresponding to 4.1% w/v in the whole reaction mixture). The mixture was stirred until the teicoplanin was dissolved and then heated at 85°C for 20 hours. The hydrolysis was monitored via HPLC every hour injecting a 30 microg. sample of the solution diluted 50 times. After cooling at room temperature the reaction mixture was poured out under stirring in 70 ml of demineralized water and the pH adjusted to 7.00 with 10% of NaOH. The obtained precipitate was collected by filtration, washed with a small amount of water on the filter and then dried at 50°C under vacuum to obtain 2.74 g (52% titre) with a molar yield of 61% crude deglucoteicoplanin. Examples 2 to 7 By substantially following the procedure of Example 1 but using the reaction conditions reported in Table I below deglucoteicoplanin was prepared: Example 8 - A) Preparation of antibiotic L 17046 (Pseudoaglycone) In a 3 l four neck round bottom flask equipped with a mechanical stirrer and a thermometer, 50 g of teicoplanin (HPLC assay 82%) were dissolved while stirring, at room temperature (22 - 24°C) in a mixture of 2.5 l of acetone and 300 ml of 30% aq. HCl. Stirring was continued for 48 hours, then the solution was cooled in an ice water bath at 3°C and slowly neutralized with 20% aq. NaOH, keeping the solution temperature under 10°C. Then the acetone was completely distilled under reduced pressure (bath temp. 40°C) and the residual water suspension, 2 l, filtered at pH = 7. The crude solid pseudoaglycone (33.2 g), was dissolved again in 1.2 l of distilled water at pH 9.5 (aq. NaOH), the resulting solution was clarified by filtration, and the pseudoaglycone precipitated again bringing the pH to 6.5 with 10% aq. HCl. After filtration the white wet solid was sludged with 200 ml of fresh acetone, filtered again, and dried for 20 hours under vacuum at room temperature. 23.5 g of the title compound was obtained (HPLC titre 70%) with a yield of 54%. The purification of the crude product was carried out according to known procedures (as described in European Patent Application Publication No. 146 053) yielding 12.8 g of 90% pure title compound. B) - Preparation of deglucoteicoplanin. By substantially following the procedure of Example 1 but using the pseudoaglycone above prepared (5 g) instead of teicoplanin complex and by employing 40 ml of DMSO as solvent, 95% H₂SO₄ (1 ml, 4.1 w/v) as mineral acid, maintaining the reaction temperature at 100°C (for 20 hours) 3,1 g (61% of molar yield) of the crude deglucoteicoplanin were prepared. Example 9 - Purification of the crude deglucoteicoplanin 1052 g of crude light brown solid deglucoteicoplanin prepared as in Example 1 were dissolved under stirring in a mixture of DMSO (7.5 l), demineralized water, (7.5 l) and 300 ml of 20% aqueous hydrochloric acid (300 ml). When the solution was complete further 4.5 l of water were added under stirring and the pH of the solution was brought to 7 using 350 ml of 20% aqueous NaOH. The suspension was then cooled to 5°C for 2 hours and the solid deglucoteicoplanin filtered and dried at room temperature in the air. 525 g of the product (HPLC assay 68%, DMSO content 17%) was obtained with yield of 65%. The mother liquors, 19 l, contained 14 g of deglucoteicoplanin (2.5%). A second portion of 1000 g of starting material was hydrolyzed and purified following the same procedure reported above. 522.5 g of deglucoteicoplanin, assay 67.5%, was obtained with a 65.3% yield. The two purified deglucoteicoplanin samples (525 plus 522.5 g) were mixed together and then suspended under vigorous stirring in 15 l of acetone. Stirring was continued for 60 minutes then the solid was recovered by filtration and dried. 960 g of product was obtained. (HPLC assay: TD 71%, H₂O 14,6%, solvents: DMSO 2.5%, acetone 9%). In order to eliminate the residual solvents, 920 g of the solid deglucoteicoplanin were suspended again in demineralized water (8.5 l) and stirred for further 4 hours. After filtering and drying under vacuum at 30°C, 805 g of 80% pure deglucoteicoplanin was obtained. The results are summarized in Table II below. EXPERIMENTAL PART HPLC analysis were performed with a Hewlett Packard mod. 1082 B apparatus equipped with a UV (254 nm) detector and a C. Erba RP 18,5 µm, 150 x 4 mm prepacked column. The mobile phase were: A) 0.025 M aqueous NaH₂PO₄/CH₃CN 95:5 (v/v) B) 0.025 M aqueous NaH₂PO₄/CH₃CN 30:70 (v/v) The chromatograms were obtained by a linear gradient elution from 8% of B in A to 75% of B in A in 45 min at a flow rate of 1.5 ml/min. The reactions were monitored by HPLC injecting at established times 30 microl. of the solution properly diluted with an acetonitrile/water mixture 2:8.

Claims: A process for transforming a teicoplanin compound or a teicoplanin-like compound into deglucoteicoplanin and its salts with bases and acids characterized in that a substance selected from teicoplanin complex, any further purified preparation thereof, teicoplanin factor A₂, teicoplanin factor A₃, each of the components of teicoplanin factor A₂, a teicoplanin-like compound of formula I wherein R is hydrogen, or a [(C₉-C₁₂)aliphatic acyl]-D-glucosamine rest; R¹ is hydrogen or a N-acetyl-D-glucosamine rest; R² is hydrogen or D-mannose rest; with the proviso that R, R¹ and R² cannot be simultaneously hydrogen, and a mixture of two or more of any of the above substances in any proportion is submitted to controlled strong acid hydrolysis in a homogeneous medium by employing an organic aprotic solvent selected from N,N-dimethylformamide (DMF), hexamethyl phosphoramide (HMPA), 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H) pyrimidone (DMPU), dimethylsulfoxide (DMSO) or mixture thereof; and a strong acid compatible with the solvent selected from a strong mineral acid and a strong organic acid. A process as in claim 1 wherein the organic aprotic solvent is dimethylsulfoxide. A process as in claims 1 or 2 wherein the mineral acid is selected from hydrochloric acid, hydrobromic acid, sulfuric acid and phosphoric acid. A process as in claims 1 to 3 wherein the mineral acid is sulfuric acid. A process as in claims 1 wherein the organic acid is selected from: methansulfonic acid, trifluoromethanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, cyclohexanesulfonic acid, camphorsulfonic acid, alpha- and beta-naphthalenesulfonic acids. A process as in claims 1 to 5 further characterized in that the reaction temperature is comprised between 60°C and 100°C. A process as in claims 1 to 5 further characterized in that reaction temperature is between 75°C and 85°C. A process as in any of the preceeding claims further characterized in that the amount of water (by weight) added to the aprotic solvent is from 1% to 20% of the solution. A process as in claims 1 to 8 further characterized in that the concentration of the acid in the whole reaction mixture ranges between 1% w/v and 10% w/v. A process as in claim 4 wherein the concentration of sulfuric acid ranges between 2% and 7% (w/v). A process according to any one of the claims 1 to 10, which further comprises dissolving the obtained deglucoteicoplanin in a solvent mixture containing DMSO/H₂O at a suitable pH, reprecipitating the product adjusting the pH of the solution at substantially neutral value and filtering the resulting pure solid. A process as in claim 11 wherein the concentration of DMSO in the DMSO/water mixture ranges between 30% and 70% (v:v). A process as in claim 11 wherein the concentration of DMSO in the DMSO/water mixture ranges between 40% and 60% (v:v). A process as in claims 11 to 13 further characterized in that the deglucoteicoplanin is dissolved in a solvent mixture containing DMSO/water at a pH brought to about 4 by addition of a strong mineral acid. A process as in claim 14 wherein the strong mineral acid is selected from hydrochloric acid, hydrobromic acid and sulfuric acid. A process as in claims 11 to 13 further characterized in that the deglucoteicoplanin is dissolved in a solvent mixture containing DMSO/water at pH brought to about 10 by addition of an alkali hydroxyde. A process as in claim 16 wherein the alkali hydroxyde is aqueous NaOH. A process as in claims 14 to 17 wherein the solid was precipitated at a pH comprised between 6 and 8. A process as in claims 14 to 17 wherein the solid is precipitated at pH 7. A process as in claims 11 to 19 which is further characterized in that a double sludge of the solid, first with acetone and then with demineralized water is carried out after the reprecipitation of the deglucoteicoplanin.

Inventors: GIANANTONIO ANACLETO, PANZONE GIAMBATTISTA, GIANANTONIO, ANACLETO, PANZONE, GIAMBATTISTA
Applicants: LEPETIT SPA, GRUPPO LEPETIT S.P.A.
Application year: 1989
Main classification: C07K9
IPC codes: C07K7, C07K14, C07K1, C07K9