AGENT FOR TREATMENT AND PROPHYLAXIS OF ISCHEMIC DISEASE OF HEART OR BRAIN

Abstract: The invention relates to an agent for treatment and prophylaxis of ischemic diseases of the heart or brain which contains as an active ingredient 2-alkynyladenosine represented by general formula (I), wherein n represents an integer of 2 to 15.

Description: FIELD OF THE INVENTION The present invention relates to the use of a 2-alkynyladenosine for the manufacture of a therepeutic agent for ischemic diseases of the heart or brain. BACKGROUND OF THE INVENTION Adenosine, which is a physiologically active substance in vivo , has a variety of physiological activities and has been known to play an important role in tissue cells or nerve functions, leading to homeostasis maintenance. However, adenosine given externally will be deaminated by adenosine deaminase so rapidly that it will immediately be inactivated. Owing to such reasons as mentioned above, various attempts have been made hitherto to synthesize adenosine derivatives for the purposes of ameliorating pharmacological activity, imparting adenosine deaminase resistance, alleviating side-effects, etc., and further attempts to develop them as pharmaceutical products have been actively made. Among them, various 2-substituted adenosine derivatives have been synthesized. For example, as typically represented by 2-methoxyphenyladenosine (Hirata, M., Kawazoe, K., Tanabe, M. and Kikuchi, K.: Japan J. Pharmacol., 27 , 689 (1977)) or 2-phenylaminoadenosine (Marumoto, R., Yoshioka, Y., Miyashita, O., Shima, S., Imai, K., Kawazoe, K. and Honjo, M.: Chem. Pharm. Bull., 23 , 759 (1975)), those exhibiting physiological activities such as adenosine deaminase inhibitory effect, coronary vasodilating effect, platelet aggregation inhibitory effect, antiviral effect, etc. have been obtained. Since adenosine has a potent vasodilating effect as well as a potent platelet aggregation inhibitory effect, there have been hitherto synthesized various adenosine derivatives including the above-mentioned derivatives for the purposes of remedying ischemic heart diseases, ischemic brain diseases, etc. However, the adenosine derivatives of the prior art still cannot be free from such side-effects as adenosine has, such as atrioventricular conduction inhibitory effect, reduction of renal blood flow amount, etc., and no excellent compounds have been found as yet. On the other hand, a 2-alkynyladenosine having a substituent introduced by carbon-carbon bond at the 2-position of adenosine is disclosed in Japanese laid-open patent publications [Japanese Laid-Open Patent Publications Nos.99395/1987 and 99330/1987/. Specifically the preparation of 2-alkynyladenosines having in the 2-position a substituent of formula C≡CR with R being CH₃(CH₂) n and n having the value 3 to 5, has been disclosed in "Nucleic Acids Research, Symposium Series" No.12 (1983), pages 5 to 8. Furthermore is is reported in "Nucleic Acids Research, Symposium Series" No.16 (1985), pages 97 to 100, that such 2-alkynyladenosines with n having values of 4,5 or 7 show potent hypertensive activity without exhibiting an allergic effect and without a heart rate dicrease.However, only the use as antihypertensive agents is described in these publications, and no other pharmacological effects, particularly ameliorating effects for ischemic diseases such as platelet aggregation inhibitory effect, coronary blood flow increasing effect, cerebral blood flow increasing effect, and brain protecting effect (e.g. anti-hypoxia effect and anti-anoxia effect), are referred to therein. Generally speaking, drugs to be used for therapy of ischemic heart diseases such as angina pectoris, heart failure, myocardial infarction, etc., as well as ischemic cerebral diseases such as cerebral circulatory disorders should desirably be compounds having platelet aggregation inhibitory effect and blood flow increasing effect or brain protecting effect at the lesion site. Therefore, in the therapy for ischemic diseases of the heart or brains it would be of great significance to provide an agent comprising an adenosine derivative having a potent platelet aggregation inhibitory effect, coronary blood flow increasing effect, cerebral blood flow increasing effect or brain protecting effect without entailing side-effects as mentioned above. SUMMARY OF THE INVENTION We have investigated 2-alkynyladenosines for their blood flow increasing effects on various blood vessels such as coronary artery, vertebral artery, etc., platelet aggregation inhibitory effect and brain protecting effect under hypoxia or anoxia. Further, from the standpoint of side-effects, we have conducted intensive studies on the appearance of atrioventricular block in the heart and the reduction of renal blood flow amount. As a result, we have found that the compounds represented by the following formula [I] have a potent platelet aggregation inhibitory effect, potent blood flow increasing effects on various blood vessels and a potent brain protecting effect in combination, and yet they do not practically cause the appearance of atrioventricular block or the reduction of renal blood flow amount which are side-effects associated with adenosine and adenosine derivatives known in the art and rather exhibit excellent characteristics for therapy for various ischemic diseases such as ischemic heart disease and ischemic brain disease, and we have eventually accomplished the present invention. More specifically, the present invention is characterized by the use of a 2-alkynyladenosine represented by the formula [I]: wherein n represents an integer of from 2 to 15, for the manufacture of a therapeutic agent for ischemic diseases of the heart or brain. DESCRIPTION OF THE PREFERRED EMBODIMENTS For the 2-alkynyladenosines represented by the formula [I], those with n in the range of from 2 to 13, more preferably from 3 to 7, should preferably be used as the active ingredients in view of the biological activities as described hereinafter. The 2-alkynyladenosines which are the active ingredients of the drugs or agent to be manufactured according to the present invention (hereinafter referred to as "the compounds used in the present invention") can be synthesized in a manner known per se by reacting a 2-halogenoadenosine represented by the formula [II]: wherein X represents iodine or bromine, with an alkyne represented by the formula [III]: CH≡C(CH₂) n CH₃ [III] wherein n represents an integer of from 2 to 15, in a solvent in the presence of bis(triphenylphosphine) palladium dichloride and cuprous iodide (see Japanese Laid-Open Patent Publications Nos. 99330/1987 and 99395/1987). The alkyne used in the reaction should be chosen to have the number of n corresponding to the desired compound to be used in the present invention. For the reaction solvent, a basic solvent can be employed, for example, a solvent mixture of triethylamine and N,N-dimethylformamide. In place of triethylamine, tertiary amines such as tributylamine, trioctylamine, N,N,N'-N'-tetramethyl-1,8-naphthalenediamine, dimethylaniline, diethylaniline, pyridine, etc. can be used, and in place of N,N-dimethylformamide, aprotic polar solvents such as N,N-dimethylacetamide, dimethyl sulfoxide, and acetonitrile can be used. The reaction terminates in several hours at room temperature to solvent reflux temperature. The compounds to be used in the present invention can be isolated by any conventional separation and purification method. For example, adsorption chromatography, ionexchange chromatography, extraction or recrystallization can be applied for the isolation. The drugs manufactured according to the present invention can be used for the purposes of therapy of ischemic heart diseases such as angina pectoris, heart failure and myocardial infarction; or ischemic cerebral diseases such as cerebral circulatory disorders due to cerebral infarction sequelae or cerebral hemorrhage sequelae, acute cerebral ischemia due to hypoxia or anoxia in the brain, etc. The respective compounds of formula [I] are used in their free forms or as acid addition salts together with pharmaceutically acceptable acids. Examples of such acid addition salts are inorganic acid salts such as hydrochloride, sulfate and hydrobromide, or organic acid salts such as oxalate, citrate and malate. For oral or parenteral administration the compound may be combined with conventional pharmaceutically acceptable carriers for therapy. The drugs suitable for oral administration can be solid form preparations such as powders, granules, capsules and tablets, or liquid form preparations such as syrups and elixirs. The drugs suitable for parenteral administration can be injections, suppositories, ointments, or inhalants. These preparations can be prepared by a conventional method by adding pharmaceutically acceptable carriers to the compounds to be used in the present invention. Further, according to the known technique, they can also be formed into sustained release preparations. In the preparation of the solid form preparation for oral administration, the compound to be used in the present invention is mixed with an excipient such as lactose, starch, crystalline cellulose, calcium lactate, calcium monohydrogenphosphate, magnesium aluminometasilicate or anhydrous silicic acid to give powders, or, if necessary, the powder is further mixed with a binding agent such as refined sugar, hydroxypropylcellulose or polyvinylpyrrolidone, or a disintegrating agent such as carboxymethylcellulose or carboxymethylcellulose calcium for wet or dry granulation to give granules. In the preparation of tablets, these powders and granules, if necessary, mixed with a lubricant such as magnesium stearate or talc may be punched into tablets. Alternatively, these granules or tablets can be coated with an enteric base such as hydroxypropylmethylcellulose phthalate or a methyl methacrylate copolymer to give enteric-coated preparations, or they can be coated with ethylcellulose, carnauba wax or a hydrogenated oil to give sustained release preparations. Further, in order to prepare capsules, the powders or granules can be charged into hard capsules, or the compound of the present invention is first dissolved in glycerol, polyethylene glycol, sesame oil or olive oil and then coated with a gelatin film to give soft capsules. In order to prepare the liquid preparation for oral administration, the compound to be used in the present invention and a sweetener such as refined sugar, sorbitol or glycerol may be dissolved in water to give a clear syrup, or the syrup may be further mixed with an essential oil or ethanol to give an elixir, or with gum arabic, tragacanth gum, polysorbate 80 or carboxymethylcellulose sodium to give an emulsion or a suspension. These liquid preparations may also contain flavoring agents, colorants, preservatives, etc., if desired. In order to prepare the injection, the compound to be used in the present invention may be dissolved in distilled water for injection, if necessary, together with a pH adjusting agent such as hydrochloric acid, sodium hydroxide, lactic acid, sodium lactate, sodium monohydrogenphosphate or sodium dihydrogenphosphate, and an isotonizing agent such as sodium chloride or glucose, aseptically filtered and charged into ampoules, or the solution may be mixed with mannitol, dextrin, cyclodextrin or gelatin and lyophilized under vacuum to give an injection which should be dissolved on use. Furthermore, the compound to be used in the present invention can be mixed with lecithin, polysorbate 80 or polyoxyethylene-hydrogenated castor oil, and the mixture is emulsified in water to give an emulsion for injection. In order to prepare the preparation for rectal administration, the compound to be used in the present invention may be melted by heating together with a suppository base such as a tri-, di- or mono-glyceride of cacao fatty acid or polyethylene glycol, poured into a mold and cooled, or the compound to be used in the present invention may be dissolved into polyethylene glycol or soybean oil and then coated with a gelatin film. In order to prepare the preparation for external application, the compound to be used in the present invention is added to white vaseline, beeswax, liquid paraffin or polyethylene glycol and the mixture is kneaded, if necessary, under heat to give an ointment, or it is kneaded with an adhesive such as rosin or an alkyl acrylate polymer, and then spread over a nonwoven fabric made of, for example, polyethylene to give a tape preparation. In order to prepare the inhalant, the compound to be used in the present invention is dissolved or dispersed in a propellant such as flon gas and charged into a pressure vessel to give an aerosol. The dose of the compound to be used in the present invention depends on the age, body weight and the conditions of disease but is generally about 0.1 mg to 100 mg per day per individual and administered desirably in one portion or several portions. Synthesis example of the compound to be used in the present invention 6.0 g of 6-chloro-2-iodo-9-(2,3,5-tri-O-acetyl-β-D-ribofuranosyl)purine was added to 60 ml of methanol/ammonia (saturated at 0°C) to cause reaction at a temperature of 60°C for 17 hours in a sealed tube. The reaction solution was cooled, then degassed, and concentrated under reduced pressure. Crystallization of the residue from water afforded 3.94 g of 2-iodoadenosine (90% yield). Melting point: 141 to 144°C. 393 mg (1 mmole) of the 2-iodoadenosine was dissolved in dimethylformamide (10 ml) - triethylamine (3 ml), and to the solution obtained were added 21 mg of bis(triphenylphosphine) palladium dichloride and 12 mg of cuprous iodide. To the resulting solution was added an alkyne (1.1 equivalent) in an argon stream, and the mixture was stirred under heat at 80°C. After the reaction solution was concentrated under reduced pressure, the residue was dissolved in methanol, and hydrogen sulfide was passed through the solution for one minute. The precipitate formed was filtered off, and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by column chromatography on silica gel and recrystallized from methanol or methanol-water to obtain 2-alkynyladenosine. The reaction time, yield, melting point and infrared absorption spectrum are shown in TABLE 1. Compound No. Species of Alkynyl Group Reaction Time (hr) Yield (%) Melting Point (°C) IR (KBr)cm⁻¹ νC≡C 1 -C≡C(CH₂)₂CH₃ 1 90 129-132 2230 2 -C≡C(CH₂)₃CH₃ 1 85 121-125 2230 3 -C≡C(CH₂)₄CH₃ 1 93 113-115 2230 4 -C≡C(CH₂)₅CH₃ 1 84 101-103 2230 5 -C≡C(CH₂)₆CH₃ 1 90 98-101 2230 6 -C≡C(CH₂)₇CH₃ 1 88 121-123 2230 7 -C≡C(CH₂)₈CH₃ - - - - 8 -C≡C(CH₂)₉CH₃ 1 97 128-130 2230 9 -C≡C(CH₂)₁₀CH₃ - - - - 10 -C≡C(CH₂)₁₁CH₃ 1 93 131-134 2230 11 -C≡C(CH₂)₁₂CH₃ - - - - 12 -C≡C(CH₂)₁₃CH₃ 3 98 134-136 2230 13 -C≡C(CH₂)₁₄CH₃ 24 80 138-139 2230 14 -C≡C(CH₂)₁₅CH₃ 24 72 131-134 2230 Pharmacological test examples Test Example 1: Platelet aggregation inhibitory effect Male Nippon white rabbits weighing 2.0 to 2.5 kg were used. Under anesthesia with pentobarbital sodium, blood was sampled from common carotid artery, and mixed with 1/7 volume of a citric acid dextrose solution which is an anticoagulant. Next, centrifugation was carried out at 140 x g for 15 minutes, and the supernatant (platelet-enriched plasma: PRP) was collected. The PRP was centrifuged at 1300 x g for 7 minutes. The platelet pellets obtained were washed with a HEPES buffer for platelet washing (pH 6.5) and centrifuged at 1300 x g for 7 minutes. The platelet pellets washed twice under the same conditions were suspended in a suspending solution (a buffer for platelet washing to which 1.0% human fibrinogen, 1.8 mM calcium chloride, 1.2 mM magnesium chloride were added) to prepare a platelet suspension to 5 to 8 x 10⁸ platelets/ml. For assaying platelet aggregation ability, an aggregometer was used, and 10 µℓ of a test drug was added to 380 µℓ of the platelet suspension. Three minutes later, 10 µℓ of a platelet aggregating substance (10 µM adenosine 5'-diphosphate at a final concentration) was added thereto to induce aggregation. The platelet aggregation inhibitory effect by the test drug is represented by the IC₅₀ value (concentration of the test drug which inhibits 50% of platelet aggregation). The results are shown in column (A) in TABLE 2. Test Example 2: Coronary artery blood flow increasing effect Adult mongrel dogs weighing 9 to 15 kg were anesthetized with pentobarbital sodium, and the chest of each was opened under artificial respiration. After pericardium excision, the left anterior descending coronary artery was peeled off from the surrounding tissues, a probe for blood flow measurement was set under heparin sodium treatment, and the coronary blood flow was measured by the use of an electromagnetic blood flow meter. The test drug was injected intraarterially into the coronary artery. The coronary artery blood flow increasing effect is represented by the ED₅₀ value (dosage when blood flow is increased by 50% by administration of the test drug when, after blocking the coronary blood flow for 15 seconds, the amount of the blood flow increased by the reactive hyperemia occurring when de-blocked is defined as 100%). The results are shown in column (B) in TABLE 2. Test Example 3: Vertebral artery blood flow increasing effect The chests of female and male adult mongrel dogs weighing 8 to 13 kg, under anesthesia by intravenous administration of pentobarbital sodium, were opened under artificial respiration along the median line to expose the left vertebral artery. Under heparin sodium treatment, a polyethylene cannula was inserted into the left vertebral artery. A probe for blood flow measurement was set at the other end of the cannula, and a tube for blood perfusion led from the left femoral artery was connected to the probe. The vertebral artery blood flow was measured by means of an electromagnetic blood flow meter. The test drug was injected intraarterially into the vertebral artery. The vertebral artery blood flow increasing effect is represented by the ED₅₀ value (dosage when blood flow is increased by 50% by administration of the test drug when the amount of the blood flow increased by vertebral administration of 100 µg/kg papaverine hydrochloride (exhibiting substantially the maximum reaction) is defined as 100%). The results are shown in column (C) in TABLE 2. (A) Platelet Aggregation Inhibitory Effect Compound No. Species of Alkynyl Group IC₅₀ (M) 2 -C≡C(CH₂)₃CH₃ 6.1 x 10⁻⁸ 4 -C≡C(CH₂)₅CH₃ 1.4 x 10⁻⁷ 5 -C≡C(CH₂)₆CH₃ 2.1 x 10⁻⁷ 6 -C≡C(CH₂)₇CH₃ 7.2 x 10⁻⁷ 12 -C≡C(CH₂)₁₃CH₃ 3.1 x 10⁻⁶ < Adenosine Control 3.2 x 10⁻⁶ (B) Coronary Artery Blood Flow Increasing Effect Compound No. Species of Alkynyl Group ED₅₀ (µg/kg) 2 -C≡C(CH₂)₃CH₃ 0.002 4 -C≡C(CH₂)₅CH₃ 0.008 5 -C≡C(CH₂)₆CH₃ 0.075 6 -C≡C(CH₂)₇CH₃ 0.15 12 -C≡C(CH₂)₁₃CH₃ 0.1 < Adenosine Control 0.1 (C) Vertebral Artery Blood Flow Increasing Effect Compound No. Species of Alkynyl Group ED₅₀ (µg/kg) 2 -C≡C(CH₂)₃CH₃ 0.14 4 -C≡C(CH₂)₅CH₃ 0.4 5 -C≡C(CH₂)₆CH₃ 1.2 6 -C≡C(CH₂)₇CH₃ 3 < 12 -C≡C(CH₂)₁₃CH₃ 3 < Adenosine Control 0.52 Test Example 4: Brain protecting effect under hypoxia or anoxia When the brain falls into hypoxia by cerebral circulatory disorder, its function will be rapidly and irreversibly lost. Accordingly, the pharmacological activities of the drugs were evaluated with the extension time relative to the survival time of the test animals under hypoxia or anoxia induced by changing gas phase components or by a drug. Experiment 1 (Normobaric hypoxia method) Evaluation of the pharmacological activity by the normobaric hypoxia method was conducted by following the method of Yasuda et al. (Arch. Int. Pharmacodyn, 233 , 136-144 (1978)) as described below. Animals used were 8 to 11 male ICR mice of 7 weeks age (Clea Japan) per group. The mice were placed in a desiccator made of glass, and hypoxia was induced by passing a gas mixture of 96% N₂ and 4% O₂ at a flow rate of 5 liters/min. The time after the passing of the gas into the desiccator to the respiration arrest of each mouse was measured and defined as the survival time. The test drug was administered intraperitoneally 30 minutes before the initiation of gas passing. The average survival times for the control group and the group to which the test drug was administered were calculated. The results are shown in column (A) in TABLE 3. The test drug was suspended in a physiological saline containing 0.5% CMC (carboxymethyl cellulose), and the dosage was 0.1 ml per 10 g of body weight. To the mice of the control group was administered 0.5% of the CMC physiological saline in the same manner. The statistical analysis was conducted according to the student's t-test. Results: Significant extension of survival time was recognized for 2-hexynyladenosine (Compound 2) by the administration of 1 mg/kg, and for 2-octynyladenosine (Compound 4) by the administration of 1 mg/kg and 3 mg/kg. Experiment 2 (Potassium cyanide (KCN)-induced anoxia method) Evaluation of the pharmacological activity by the KCN-induced anoxia method was conducted according to the Karasawa et al. procedure (J. Pharmacobio-Dyn. 5 , 295-300 (1982)). Animals used were 13 to 15 male ICR mice of 8 weeks age (Clea Japan) per group. The test drug was administered intraperitoneally at a dose of 0.1 ml per 10 g of body weight, and 30 minutes later, 2.5 mg/kg of KCN was administered rapidly into a tail vein. The survival time was measured. The test drug was prepared in the same manner as in Experiment 1. The average survival times for the control group and the group to which the test drug was administered were calculated. The results are shown in column (B) in TABLE 3. The mice which exhibited no respiration arrest over 180 seconds or longer after KCN administration were rated as survival examples, and the survival time was calculated as 180 seconds in calculating the average survival time. Results: By the administration of 1 mg/kg of 2-hexynyladenosine (Compound 2) and 30 mg/kg of 2-hexadecynyladenosine (Compound 12), significant extension effects were exhibited as compared with the survival times of the respective control groups. (A) Normobaric Hypoxia Method Compound No. Species of Alkynyl Group Dose (mg/kg) Number of Test Animal Average Survival Time (sec.) 2 -C≡C(CH₂)₃CH₃ 0.1 9 171.9 (±22.1) 0.3 9 169.2 (±23.5) 1.0 9 217.3 (±26.2) 4 -C≡C(CH₂)₅CH₃ 0.3 9 128.7 (±4.8) 1.0 9 222.9 (±25.9) 3.0 9 230.6 (±26.7) Control - 11 126.8 (±10.7) (B) Potassium Cyanide Induced Anoxia Method Compound No. Species of Alkynyl Group Dose (mg/kg) Number of Test Animal Average Survival Time (sec.) 2 -C≡C(CH₂)₃CH₃ 1.0 15 50.5 (±3.8) Control (1) - 15 39.8 (±2.4) 12 -C≡C(CH₂)₁₃CH₃ 30 15 76.7 (±11.7) Control (2) - 13 41.8 (±1.9) Test Example 5: Effect on renal blood flow Female and male adult mongrel dogs weighing 10 to 15 kg were used. After each dog was anesthetized by intravenous administration of 30 mg/kg of pentobarbital sodium, it was fixed on the back, and the left lower abdominal portion was cut open under artificial respiration to expose the left renal artery. Under heparin sodium treatment, a polyethylene cannula was inserted into the renal artery, and at the other end of the cannula was set a probe for blood flow measurement. Self-perfusion was effected by means of a tube for blood perfusion led from the left femoral artery and connected to the probe, and the renal artery blood flow was measured by means of an electromagnetic blood flow meter. The test drug was injected intraarterially into the renal artery. The renal blood flow reduction effect is represented by the ID₅₀ value (dosage when blood flow is reduced by 50% by the test drug administration). The results are shown in column (A) in TABLE 4. Test Example 6: Effect on atrioventricular conduction Each of male guinea pigs weighing 360 to 600 g, after being anesthetized by intraperitoneal administration of 1.4 g/kg of urethane, was fixed on the back, and the trachea was cut open for ensuring airway, a polyethylene tube being inserted thereinto. The blood pressure was measured through a carrier amplifier by connecting the cannula inserted into the left common carotid artery to a pressure transducer. The electrocardiogram was recorded by means of a bioelectric amplifier according to the second induction method, and the PQ interval was measured from the electrocardiogram. The test drug was administered through the left femoral vein, and the presence or absence of appearance of atrioventricular block is represented by the appearance ratio of the second degree of atrioventricular block (number of animals showing appearance/number of animals used) by the test drug administration. The results are shown in column (B) in TABLE 4. (A) Renal Blood Flow Reduction Effect Compound No. Species of Alkynyl Group ID₅₀ (µg/kg) 2 -C≡C(CH₂)₃CH₃ 0.15 4 -C≡C(CH₂)₅CH₃ 3 < 5 -C≡C(CH₂)₆CH₃ 3 < 6 -C≡C(CH₂)₇CH₃ 3 < 12 -C≡C(CH₂)₁₃CH₃ 3 < Adenosine Control 0.2 (B) Atrioventricular Block Appearance Ratio Compound No. Species of Alkynyl Group Number of Animals Showing Appearance/Number of Animals Used 2 -C≡C(CH₂)₃CH₃ 6/6 4 -C≡C(CH₂)₅CH₃ 1/6 5 -C≡C(CH₂)₆CH₃ 0/6 6 -C≡C(CH₂)₇CH₃ 1/6 12 -C≡C(CH₂)₁₃CH₃ 0/6 Adenosine Control 6/6 Test Example 7: Acute toxicity By the use of Slc:ICR mice (7 to 8 weeks old), the acute toxicity tests of Compound 4 (n = 5) and Compound 12 (n = 13) were conducted. For all of the compounds, no lethal case was recognized by oral administration of the pharmaceutically and physically acceptable maximum amount of 1000 mg/kg, and the LD₅₀ value was estimated to be 1000 mg/kg or higher in each case. Example of preparation formulation Example 1 Compound (6) 25 mg Potato starch 150 mg Soft silicic acid anhydride 50 mg Magnesium stearate 10 mg Lactose 765 mg Total: 1000 mg The above components were uniformly mixed and placed into hard capsules each in an amount of 200 mg. Example 2 Compound (4) 25 mg Potato starch 150 mg Crystalline cellulose 60 mg Soft silicic acid anhydride 50 mg Hydroxypropyl cellulose 30 mg Magnesium stearate 15 mg Lactose 670 mg Total: 1000 mg Compound (4), lactose, potato starch, crystalline cellulose and soft silicic acid anhydride were mixed, and 10% methanolic solution of hydroxypropyl cellulose was added. The mixture was granulated by kneading and then extruded through a screen with a diameter of 0.8 mm to prepare granules. After drying, magnesium stearate was added, and the mixture was compression molded into tablets each of 200 mg. Example 3 Compound (2) 25 mg Propylene glycol Total: 10 ml Compound (2) was dissolved in propylene glycol, and the solution was aseptically filtered and then placed into ampoules each in an amount of 0.2 ml. Example 4 Compound (5) 25 mg Polyethylene glycol 1500 3000 mg Polyethylene glycol 6000 1975 mg Total: 5000 mg The above components were mixed uniformly by heating and melting at 60°C. The mixture was cast into a plastic mold and then cooled to form a suppository of 1 g. As described above, the 2-alkynyladenosine, which is the active ingredient of the drug to be manufactured according to the present invention, has a remarkable platelet aggregation inhibitory effect, blood flow increasing effect of various blood vessels (coronary artery and vertebral artery) and brain protecting effect in combination. It further has weak side-effects such as appearance of atrioventricular block in myocardium and reduction effect of renal blood flow, possessed by adenosine or adenosine derivatives known in the art, thus overcoming the drawbacks of the prior art. To describe in more detail, the 2-alkynyladenosine, which is the active ingredient of the present drug, has an extremely high ratio of the primary effects such as platelet aggregation inhibitory effect, blood flow increasing effect of various blood vessels, and brain protecting effect to the side-effects such as atrioventricular block appearance ratio and renal blood flow reduction effect, that is, the 2-alkynyladenosine has an extremely broad margin of safety as compared to the prior compounds. For example, the margins of safety (coefficients) of the 2-alkynyladenosine calculated on the basis of the results in TABLE 2 and TABLE 4 by taking an example of coronary artery blood flow increasing effect as the primary effect and renal blood flow reduction effect as the side-effect are as shown below in TABLE 5. From TABLE 5, it can be understood that the 2-alkynyladenosine is a compound having a broader margin of safety as compared with adenosine. Compound No. Species of Alkynyl Group Safety Coefficient 2 -C≡C(CH₂)₃CH₃ 75 4 -C≡C(CH₂)₅CH₃ 375 < 5 -C≡C(CH₂)₆CH₃ 40 < 6 -C≡C(CH₂)₇CH₃ 20 < 12 -C≡C(CH₂)₁₃CH₃ 30 Adenosine Control 2 Although it can be seen from TABLE 2 and TABLE 4 that the 2-alkynyladenosine is equal to or higher than adenosine in the primary effects, the atrioventricular block appearance ratio which is one of the side-effects is suppressed at a low level. In this connection, Compound No. 2 has the same atrioventricular block appearance ratio as that of adenosine which is the control drug, but this has been brought about by administration of a dose as large as 300 µg/kg (intravenous administration), and in view of the strength of the primary effects of said compound, it may be considered that the atrioventricular block appearance can be suppressed when this compound is administered in an effective amount for activity appearance of the primary effects. INDUSTRIAL APPLICABILITY According to the present invention, it has become possible for the first time to manufacture a drug which is extremely excellent and significant for therapy of ischemic heart diseases such as angina pectoris, heart failure, myocardial infarction, etc. and ischemic cerebral diseases such as cerebral circulatory disorders due to cerebral infarction sequelae or cerebral hemorrhage sequelae.

Claims: The use of a 2-alkynyladenosine represented by the following formula [I]: wherein n represents an integer of from 2 to 15, for the manufacture of a therapeutic agent for ischemic diseases of the heart or brain. The use according to claim 1 of a 2-alkynyladenosine of formula [I] wherein n is within the range of from 2 to 13. The use according to claim 1 or 2 of a 2-alkynyladenosine of formula [I] wherein n is within the range of from 3 to 7.

Inventors: ABIRU TOICHI, KOGI KENTARO TOA EIYO LTD KENK, YAMAGUCHI TOYOFUMI, ABIRU, TOICHI, KOGI, KENTARO TOA EIYO LTD. KENKYUSHO, YAMAGUCHI, TOYOFUMI
Applicants: TOA EIYO LTD, YAMASA SHOYU KK, TOA EIYO LTD., YAMASA SHOYU KABUSHIKI KAISHA
Application year: 1989
Main classification: A61K31
IPC codes: C07H19