1,2,4-BENZOTRIAZINE OXIDES AS RADIOSENSITIZERS AND SELECTIVE CYTOTOXIC AGENTS

Abstract: A method of using 1,2,4-benzotriazine oxides, some of which are novel compounds, as radiosensitizers and selective cytotoxic agents is disclosed. These compounds are shown to specifically radiosensitize hypoxic tumor cells. Some are additionally disclosed to be useful as specific cytotoxic agents for these cells. They also show an unexpected ability to radiosensitize aerobic cells following or preceding a hypoxic incubation of the cells with the drug. This provides a basis for selective radiosensitization of tumors compared to normal cells. A novel method for preparing the 1,2,4-benzotriazine oxides is also disclosed.

Description: 1,2,4-BENZOTRIAZINE OXIDES AS RADIOSENSITIZERS AND SELECTIVE CYTOTOXIC AGENTS Technical Field The invention relates to cytotoxic agents and radiotherapy effective against hypoxic cells. More specifically, the invention relates to certain novel 1,2,4-benzotriazine oxides, to methods of selectively killing tumor cells and/or sensitizing tumor cells to radiation using selected 1,2,4-benzotriazine oxides, and to novel synthetic methods. Backσround Art Hypoxic cell radiosensitizers are compounds that selectively increase the sensitivity of hypoxic cells to destructive radiation. Cytotoxins which have enhanced activity under hypoxic conditions also provide a means for selective destruction of cells under low oxygen pressure. This specificity for hypoxic cells is important because it is tumors that are typically characterized by such cells. Virtually all tumors which are present as solid masses contain these cells, while normal cells generally have an adequate supply of oxygen. Accordingly, anti-tumor agents can be made selective for tumors by virtue of high activity under hypoxic conditions, and radiation can be employed more effectively in the presence of these sensitizers. Of course, the use of radiation treatment to destroy tumor cells is only practical if damage to the surrounding normal tissue can be minimized or avoided. The effects of radiation are enhanced by the presence of oxygen, and it is established that as the dose of radiation is increased, the effectiveness of the radiation in destroying target cells is enhanced most dramatically when oxygen is present. Therefore, selectivity for tumor cells toward radiation is difficult to achieve — normal cells, in view of their oxygep supply, are generally more susceptible to radiation than the target tumor cells. It is therefore desirable to provide a means of sensitizing tumor cells, but not the surrounding tissue, to radiation treatment. One solution would be to increase the supply of oxygen to these tumor cells. This, however, has proved difficult to do. Various heterocyclic compounds and in particular those with oxidized nitrogen moieties, have been used to radiosensitize hypoxic tumor cells. Indeed it has been postulated that the oxidized nitrogen functionality is responsible for this activity. Nitroimidazoles, particularly misonidazole (MIS) and metronidazole have been studied extensively, and MIS i commonly used as a standard in in vitro and .in vivo tests for radiosensitizing activity. (See, e.g., Asquith, et al. Radiation Res (1974) 6O.108-118 Hall, et al, Brit J Cancer (1978) 3J 567-569; Brown, et al 1 . Radiation Res (1980) .82:171-190; and U.S. patent 4,371,540. The radiosensitizing activities of certain 1-substituted 3(5)-nitro-s-triazoles and of various quinoxaline-l,4-dioxide derivatives have also been disclosed. In addition, US Serial Nos. 730,761, filed 3 May 1985, and 788,762, filed 18 October 1985 assigned t the same assignee and incorporated by reference disclos a group of radiosensitizers that do not contain oxidize nitrogen — the substituted benzamides and nicotinamides and their thio analogs. These compounds, nevertheless, are radiosensitizers. It is important to distinguish the ability, to sensitize hypoxic cells selectively, for instance, by enhancing their oxygen supply, from another mechanism commonly encountered for "sensitizing" cells: inhibition of the enzyme poly(ADP-ribose)polymerase, which is believed to be essential in the repair of irradiated cells after radiation. This repair mechanism is operative in both hypoxic tumor cells and in normal cells. Hence, administration of "radiosensitizers" which operate according to this latter mechanism does not accomplish the desired purpose of selectively sensitizing the target tumor cells. A group of compounds which has not previously been suggested for use in either selectively killing hypoxic cells or in radiosensitizing such cells is 3-amino-l,2,4-benzotriazine 1,4-di-N-oxide and related compounds. Related US patents 3,980,779; 3,868,371; and 4,001,410 disclose the preparation of a group of these compounds and their use as anti-microbial agents, particularly by addition of these materials to livestock fodder. U.S. Patent Nos. 3,991,189 and 3,957,799 disclose derivatives of these compounds bearing substituents on the nitrogen of the 3-amino group. These compounds also have anti-microbial activity. The present invention provides additional compounds which specifically radiosensitize hypoxic cells and which, furthermore, are directly cytotoxic to hypoxic cells both jln vitro and .in vivo. Therefore, administration of these compounds prior to or following radiation treatment of tumors selectively kills the hypoxic (tumor) cells which survive the radiation dose. Both the ability of these compounds to radiosensitize hypoxic cells and especially their ability to selectively kill hypoxic cells directly are unexpected properties of these compounds. The invention also provides novel 1,2,4-benzotriazine oxides useful as radiosensitizers and/or selective cytotoxic agents; methods of synthesizing the compounds; and methods of administering the compounds to achieve radiosensitization and/or selective cell killing. Disclosure of the Invention The invention provides a valuable addition to the group of compounds currently available as selective radiosensitizers and selective cytotoxic agents for hypoxic tumor cells. Some of the compounds now newly shown to be useful in this regard are known compounds. Others are themselves novel. Accordingly, one aspect of the invention is a method of radiosensitizing hypoxic tumor cells by administering to these cells a compound of the formula: 0 wherein X is H; hydrocarbyl (1-4C); hydrocarbyl (1-4C) substituted with OH, NH2, NHR or NRR; halogen; OH; alkoxy (1-4C); NH2; NHR or NRR; wherein the various R groups are independently selected from lower alkyl (1-4C) and lower acyl (1-4C) and the R's may themselves be substituted with OH, NH2, alkyl (1-4C) secondary and dialkyl (1-4C) tertiary amino groups, alkoxy (1-4C) or halogen. In the case of NRR, the two R's 'can be linked together directly or through a bridge oxygen into a morpholino ring, pyrrolidino ring or piperidino ring; n is 0 or 1; and Y~ and Y are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y 1 and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R* , NH(CO)R' , 0(SO)Rl' . or 0(POR* )R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents, or pharmacologically acceptable salts of said compound. In another aspect, the invention provides an improved method of fractionated radiotherapy which involves treating the cells requiring radiotherapy with a 1,2,4-benzotriazine oxide of Formula (I), as just defined, before or after subjecting the treated cells to a plurality of distinct radiation doses over an extended period of time, each of the radiation doses being less than about 5 Gy. The compounds useful in conjunction with the presently disclosed radiosensitizing methods, therefore, are the mono- or dioxides of optionally substituted 1,2,4-benzotriazine which may contain a hydrocarbyl (1-4C), hydroxyl, alkoxy or amino group, either substituted or unsubstituted, in the 3-position, and their pharmacologically acceptable salts as set forth i Formula I The invention also provides a method for selectively killing hypoxic tumor cells using certain o these 1,2,4-benzotriazine oxides. The compounds which are useful as selective cytotoxic agents are a subset o the above-defined compounds useful as radiosensitizers. That is, while all of the compounds defined by Formula (I) are generally effective as radiosensitizers, only those compounds unsubstituted at the 3-position or having a 3-amino or 3-hydrocarbyl (1-4C) substituent (i.e., X=H, hydrocarbyl (1-4C), H2, NHR or NRR with each R as defined above) and which are di-N-oxides (n l) are effective cytotoxic agents. In this aspect, the invention provides a method of selectively killing hypoxic tumor cells by administering one or more of these compounds (or its salts) to the hypoxic tumor cells. Certain of the compounds encompassed by Formula (I) are already known in the art as being useful for other purposes; other compounds are novel. The novel compounds encompassed by the present invention an which may be prepared by methods disclosed herein include compounds represented by Formula (I), in which the 'substituents fall into the following three classes: I. X is OH, alkoxy (1-4C), NHR or NRR where each R is independently an alkyl of 1-4 carbon atoms, or acyl of 1-4 carbon atoms, or where the two R groups are alkyls linked together to form a pyrrolidino or piperidino ring or linked through an oxygen to form a morpholino ring, and the R groups may be further substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; n is 1; and Y 1 and Y are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetyla inoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y x and Y are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(C0)R ? , NH(C0)R*, 0(S0)R' , or 0(POR" )R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents. Pharmacologically acceptable salts of these compounds are ^.lso included in this class of compounds. II. X is NH2; n is 1; and Y 1 and Y 2 are chosen such that one but not both may be hydrogen and one or both may independently be either nitro, saturated or unsaturated hydrocarbyl of 7-14C, or unsaturated hydrocarbyl of 2-6C, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) and alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y^ and Y are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R* , NH(CO)R' , 0(S0)R' , or 0(POR' )R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents. Pharmacologically acceptable salts of these compounds are also included in this class of compounds. III. X is hydrogen or hydrocarbyl (2-4C) optionally substituted with OH, NH2, alkoxy (1-4C) or halogen substituents; ^ n is 1; and Y 1 and Y 2 are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) 1 o linkage; or wherein Y and Y* are independently either morpholino, pyrrolidino, piperidino, NH2, NHR 1 , NR'R 1 0(CO)R' , NH(C0)R' , 0(SO)R' , or 0(POR' )R' in which R* is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents. Pharmacologically acceptable salts of these compounds are also included in this class of compounds. The invention also provides a straightforward, one-step synthesis for preparing 1,2,4-benzotriazine oxides unsubstituted at the 3-position (i.e., the compounds of Formula (I) wherein X=H) by treating the corresponding 3-amino-l,2,4-benzotriazine oxide with a lower alkyl nitrite under reductive deaminating conditions. Brie,f Description of the Drawings Figures 1A, IB and IC show the selective cytotoxicity of 3-amino-l,2,4-benzotriazine 1,4-dioxide for hypoxic cells derived from hamster, mouse and human tissues. Figure 2 shows the in vivo efficacy of 3-amino-l,2,4-benzotriazine 1,4-dioxide in enhancing the killing of tumor cells when combined with radiation. Figure 3 shows the killing of tumor cells in vivo by 3-amino-l,2,4-benzotriazine 1,4-dioxide when the tumor has been made hypoxic by the intraperitoneal administration of the antihypertensive drug hydralazine. Figure 4 is a graph illustrating aerobic radiosensitization of CHO cells by hypoxic pretreat ent or post-treatment with 3-amino-l,2,4-benzotriazine 1,4-dioxide, as described in Example 22. Figure 5 illustrates tumor cell survival of SCCVII tumor irradiated with 8x2.5 Gy in four days, also as described in Example 22. Figure 6 graphically illustrates the growth delay of SCCVII tumors irradiated with 8x2.5 Gy in four days, also as described in Example 22. Figure 7 shows in graph form the average skin reaction of normal mouse skin irradiated with 8 fractions (every 12 hours) of between 3 and 6 Gy per fraction. Modes of Carrying Out the Invention A. The Compounds Useful in the Invention The compounds useful as radiosensitizers and selective cytotoxic agents as described herein are derivatives of 1,2,4-benzotriazine oxide represented by Formula (I). These compounds, as shown in the Formula (I), contain a group X in their 3 position. X will vary specifically as set forth above, depending upon the activity desired. Subject to the above-recited selection criteria, X is chosen in general from hydrogen; unsubstituted hydrocarbyls (1-4C) such as methyl, ethyl, s-butyl and the like; hydroxy; alkoxy (1-4C) such as methoxy, ethoxy, propoxy, t-butoxy and the like; primary amino (NH2); secondary amino (NHR) where R is an alkyl or acyl of 1 to 4 carbons, such as methylamino, ethylamino and the like; tertiary amino (NRR) where each of the R groups is an alkyl or acyl of 1 to 4 carbons, for example diethylamino and the like, or the two R's join to form a morpholino, pyrrolidino or piperidino ring. In the case of the various alkyl and acyl R groups, they can be further substituted with OH, NH2, lower alkyl (1-4C) secondary amino and dialkyl (1-4C) tertiary amino, morpholino pyrrolidino, piperidino, alkoxy (1-4C) or halogen (fluoro, chloro, bromo or iodo) substituents. In the case of the hydrocarbyl X groups, they can be further substituted with OH, NH2, alkyl secondary amino, dialkyl tertiary amino, alkoxy (1-4C) or halogen (fluoro, chloro, bromo or iodo) substituents. The compounds of Formula (I) additionally contain groups Y 1 and Y . These groups are selected specifically according to the criteria set forth above, depending upon the utility desired. Subject to these criteria, Y 1 and Y 2 may be selected from hydrogen; nitro; halogen (e.g. fluoro, chloro, bromo or iodo); or hydrocarbyl (1-14C). When hydrbcarbyl, Y 1 or Y 2 may be saturated or unsaturated, cyclic or acyclic, and may optionally be interrupted by a single ether linkage. Thus, the unsubstituted hydrocarbyl forms of Y 1 or Y 2 can be, for example, methyl, ethyl, n-propyl, s-butyl, n-hexyl, 2-methyl-n- pentyl, 2-ethoxyethyl, 3-(n-propoxy)-n-propyl, -methoxybutyl, cyclohexyl, tetrahydrofurfuryl, furfuryl, cyclohexenyl, 3-(n-decyloxy)-n-propyl, 4-methyloctyl, 4,7-dimethyloctyl, or the like. The hydrocarbyl Y and Y 2 groups may optionally be substituted with 1 or 2 substituents selected from halogen such as fluoro, chloro, bromo or iodo; hydroxy; epoxy; alkoxy (1-4C) such as, for example, methoxy, n-propoxy and t-butoxy; alkyl thio; (1-4C) primary amino (NH2); morpholino; pyrrolidino; piperidino; secondary amino (NHR* ) where R' is a 1-4C alkyl, such as methylamino, propylamino and the like; tertiary amino (NR'R'); acyloxy and acylamido groups represented by R'COO- and R'CONH-, respectively, and their thiol analogs represented by R'CSO- and R'CSNH- respectively; carboxy (-C(O)OH); alkoxycarbonyl (-C(O)OR' ); carbamyl (-C(0)NH2); alkylcarbamyl (1-4C) (-C(O)NHR'); alkylsulfonyl (1-4C) (R'Sθ2-); and alkyl phosphonyl (1-4C) ( R'P(OR')O-). In addition Y~ and Y 2 can each independently be -NH2, -NHR', -NR'R', -OCOR' , -NH(CO)R' , -0(SO)R' or -0(POR' )R* in which the various R' groups are lower alkyls (1-4C) which themselves may be substituted with OH, NH2» alkyl secondary and tertiary amino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents. A particularly promising class of compounds for use both as radiosensitizers and selective cytotoxic agertts include those represented by the following structural Formula (II): In Formula (II), one of Y and Y 2 is H, the other an electron-withdrawing group (e.g., nitro, carboxy, alkoxycarbonyl, alkylsulfonyl), Ri and R2 are independently selected from the group consisting of hydrogen and lower alkyl, or the Ri and R2 groups may be linked to form a piperidino or pyrrolidino ring, and m is an integer from 0-4 inclusive, preferably 1 or 2. Where X is OH, of course, the compounds may also be prepared and used as the pharmaceutically acceptable salts formed from inorganic bases, such as sodium, potassium, or calcium hydroxide, or from organic bases, such as caffeine, ethylamine, and lysine. When X is NH2, NHR, or NRR, e.g., NH-CH2- (CH2)m~CH2 RιR2 as in Formula ( II), pharmaceutically acceptable acid addition salts may be used. These salts are those with inorganic acids such as hydrochloric, hydrobromic or phosphoric acids or organic acids such as acetic acid, pyruvic acid, succinic acid, mandelic acid, p-toluene sulfonic acid, and so forth. (Amino substituents on the hydrocarbyl side chain can also, of course, be converted to salts.) The 1,2,4-benzotriazines may be usedin the practice of this invention as the mono- or dioxides; i.e. either the 1-nitrogen of the triazino ring may be oxidized, or both the 1-and 4-nitrogens may be oxidized. Specific particularly preferred compounds which are useful in the radiosensitization and cytotoxic procedures of the invention include 3-hydroxy-l,2,4^5enzotriazine 1-oxide; 3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 3-amino-l,2,4-benzotriazine 1-oxide; 3-amino-l,2,4-benzotriazine 1,4-di-oxide; 6(7)-methoxy-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-methoxy-3-hydroxy-l,2, -benzotriazine 1, -dio ide; 6(7)-methoxy-3-amino-l,2, -benzotriazine 1-oxide; 6(7)-methoxy-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-ethoxy-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-ethoxy-3-hydroxy-l,2,4-benzotriazine 1, -dioxide; 6(7)-ethoxy-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-ethoxy-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[4-acetamido-n-butanoxy]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-[4-acetamido-n-butanoxy]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[4-acetamido-n-butanoxy]-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[4-acetamido-n-butanoxy]-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[l-(2,3-dihydroxy)propoxy]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-[1-(2,3-dihydroxy)propox ]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[1-(2,3-dihydroxy)propox ]-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[1-(2,3-dihydroxy)propoxy]-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[(2-furyl)methylamino]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-[(2-furyDmethylamino]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[(2-furyDmethylamino]-3-amino-1,2,4- benzotriazine 1-oxide; 6(7)-[(2-furyl)methylamino]-3-amino-l,2,4-benzotriazine 1,4-dioxide; (7)-(2-methoxyethylamino)-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-(2-methoxyethylamino)-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-(2-methoxyethylamino)-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-(2-methoxyethylamino)-3-amino-l,2, -benzotriazine 1,4-dioxide; 6(7)-carbethoxymethoxy-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-carbethoxvmethoxy-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-carbethoxymethoxy-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-carbethoxymethoxy-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[(2-methoxyethyl)carbamylmethoxy]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-[(2-methoxyethyl)carbamylmethoxy]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[(2-methoxyethyl)carbamylmethox ]-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[(2-methoxyethyl)carbamylmethox 3-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[(2-hydroxyethyl)carbamylmethox ]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7 ) - • [(2-hydroxyethyl)carbamylmethoxy]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[(2-hydroxyethy1)carbamylmethox ]-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[(2-hydroxyethyl)carbamylmethox ]-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[l-(2-hydroxy-3-morpholino)propoxy]-3-hydroxy- 1,2,4-benzotriazine 1-oxide; 6 ( 7 ) - [l- (2-hydroxy-3-morpholino)propoxy]-3-hydroxy- 1,2,4-benzotriazine 1,4-dioxide; 6(7)-[l-(2-hydroxy-3-morpholino)propoxy]-3-amino-l,2,4 benzotriazine 1-oxide; 6(7)-[l- ( 2-hydroxy-3-morpholino)propoxy]-3-amino-l,2,4 benzotriazine 1,4-dioxide; 6(7)-[3-amino-n-propoxy]-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-[3-amino-n-propoxy]-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[3-amino-n-propox ]-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-[3-amino-n-propoxy]-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[2,3-epoxypropoxy]-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-[2,3-epoxypropoxy]-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[2,3-epoxypropoxy]-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-[2,3-epoxypropoxy]-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[3-methoxy-2-hydroxy-n-propoxy]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)»-[3-methoxy-2-hydroxy-n-propoxy]-3-hydroxy-l,2,4- _ benzotriazine 1,4-dioxide; 6(7)-[3-methoxy-2-hydroxy-n-propoxy]-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[3-methoxy-2-hydroxy-n-propoxy3-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[4-ethoxy-3-hydroxy-n-butoxy]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-[4-ethoxy-3-hydroxy-n-butoxy3-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[4-ethoxy-3-hydroxy-n-butoxy]-3-amino-l,2,4- benzotriazine 1-o ide; 6(7)-[4-ethoxy-3-hydroxy-n-butoxy]-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[3,4-dihydroxy-n-butox ]-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-[3, -dihydroxy-n-butoxy]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[3,4-dihydroxy-n-butoxy]-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[3,4-dihydroxy-n-butoxy3-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-methyl-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-methyl-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-methyl-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-methyl-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-ethyl-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-ethyl-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-ethyl-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-ethyl~3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-chloroacetamido-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-chloroacetamido-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)^chloroacetamido-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-chloroacetamido-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[(2-hydroxyethyloxy)acetamido3-3-hydroxy-l,2,4- benzotriazine 1-oxide; 6(7)-C(2-hydroxyethyloxy)acetamido]-3-hydroxy-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[(2-hydroxyethyloxy)acetamido3-3-amino-l,2,4- benzotriazine 1-oxide; 6(7)-[(2-hydroxyethyloxy)acetamido3-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6,7-dimethoxy-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6,7-dimethoxy-3-hydroxy-l,2,4-benzotriazine 1,4-dioxid 6,7-dimethoxy-3-amino-l,2,4-benzotriazine 1-oxide; 6,7-dimethoxy-3-amino-l, ,4-benzotriazine 1,4-dioxide; 6,7-diethoxy-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6,7-diethoxy-3-hydroxy-l,2,4-benzotriazine 1, -dioxide 6,7-diethoxy-3-amino-l,2,4-benzotriazine 1-oxide; 6,7-diethoxy-3-amino-l, ,4-benzotriazine 1 ,4-dioxide; 6(7)-propionyl-3-hydroxy-l ,2,4-benzotriazine 1-oxide; 6(7)-propionyl-3-hydroxy-l , 2,4-benzotriazine 1,4-dioxide; 6(7)-propionyl-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-propionyl-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-(2-acetoxyethoxy)-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-(2-acetoxyethox )-3-hydroxy-l,2, -benzotriazine 1,4-dioxide; 6(7)-(2-acetoxyethoxy)-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-(2-acetoxyethoxy)-3-amino-l,2,4-benzotriazine « 1,4-dioxide; 6(7)-n-hexyloxy-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-n-hexyloxy-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-n-hexyloxy-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-n-hexyloxy-3-amino-l,2,4-benzotriazine 1,4-dioxide 6(7)-ethylamino-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7)-ethylamino-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; (7 -ethylamino-3-amino-l,2,4-benzotriazine 1-oxide; (7 -ethylamino-3-amino-l,2,4-benzotriazine 1,4-dioxide; (7 -(2-methoxyethoxy)-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7 -(2-methoxyethoxy)-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7 -(2-methoxyethox )-3-amino-l,2,4-benzotriazine 1-oxide; 6(7 -(2-methoxyethoxy)-3-amino-l,2 , -benzotriazine 1,4-dioxide; 6(7 -(aminoacetamido)-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7 -(aminoacetamido)-3-hydroxy-l,2,4-benzotriazine ' 1,4-dioxide; 6(7 -(aminoacetamido)-3-amino-l,2,4-benzotriazine 1-oxide; 6(7 -(aminoacetamido)-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7 -(carbamylmethoxy)-3-hydroxy-l,2,4-benzotriazine 1-o ide; 6(7 -(carbamylmethoxy)-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7 -(carbamylmethoxy)-3-amino-l,2,4-benzotriazine 1-oxide; 6(7 -(carbamylmethoxy)-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7 -(carboxymethoxy)-3-hydroxy-l,2,4-benzotriazine 1-oxide; 6(7 -(carboxymethoxy)-3-hydroxy-l,2,4-benzotriazine 1,4-dioxide; 6(7 -(carboxymethox )-3-amino-l,2,4-benzotriazine 1-oxide; 6(7)-(carboxymethoxy)-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[1,2-dihydroxyethyl]-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[1-(3-ethylamino-2-hydroxypropoxy) ]-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[2-ethylamino-l-hydroxyethyl]-3-amino-l,2,4- benzotriazine 1,4-dioxide; 6(7)-[2-hydroxyethyl3-3-amino-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[l-hydroxyethyl]-3-amino-l,2,4-benzotriazine 1,4-dioxide; 3-(2-hydroxyethylamino)-1,2,4-benzotriazine 1-oxide; 3-(2-hydroxyethylamino)-1,2,4-benzotriazine 1,4-dioxide; 6(7)-chloro-3-(2-hydroxyethylamino)-1,2, -benzotriazine 1-oxide; 6(7)-chloro-3-(2-hydroxyethylamino)-1,2,4-benzotriazine 1,4-dioxide; 3-(1-hydroxyethylamino)-1,2,4-benzotriazine 1-oxide; 3-(l-hydroxyethylamino)-l,2,4-benzotriazine 1,4-dioxide; 1,2,4-benzotriazine 1-oxide; 1,2,4-benzotriazine 1,4-dioxide; 3-meithyl-l,2,4-benzotriazine 1,4-dioxide; 3-et^ιyl-l,2,4-benzotriazine 1,4-dioxide; 3-propyl-l,2, -benzotriazine 1,4-dioxide; 6(7)-amino-1,2,4-benzotriazine 1,4-dioxide; 6(7)-amino-3-methyl-l,2,4-benzotriazine 1,4-dioxide; 6(7)-amino-3-ethyl-l,2,4-benzotriazine 1,4-dioxide; 6(7)-methoxy-l,2,4-benzotriazine 1,4-dioxide; 6(7)-methoxy-3-methyl-l,2,4-benzotriazine 1,4-dioxide; 6(7)-[1-(2,3-dihydroxypropox 3-1,2,4-benzotriazine 1,4-dioxide; 6(7)-[1,2-dihydroxyethyl3-1,2,4-benzotriazine 1,4-dioxide; 6(7)-[1-(3-ethylamino-2-hydroxypropoxy) 3-1 ,2,4- benzotriazine ,4-dioxide; 6(7)-[2-ethylamino-l-hydroxyethyl]-l, ,4-benzotriazine 1-4 dioxide; 6(7)-chloro-1,2,4-benzotriazine 1,4-dioxide; 6(7)-[2-hydroxyethyl3-1,2,4-benzotriazine 1 ,4-dioxide; 6(7)-[1-hydroxyethyl3-1,2,4-benzotriazine 1,4-dioxide; 6(7)-nitro-3-amino-l,2,4-benzotriazine 1,4-dioxide; 3-(3-N,N-diethylaminopropylamino)-1,2,4-benzotriazine 1,4-dioxide; 6(7)-nitro-3-(2-N,N-diethylarainoethylamino)-1,2,4- benzotriazine 1,4-dioxide and their pharmaceutically acceptable salts and the thioamide analogs of the foregoing list of compounds. It should be noted that the Y 1 or Y 2 substituents set forth in most of the above compounds as present in either the 6 or 7 positions (designated "6(7)") or in both the 6 and 7 positions (designated "6,7") may also be present at the 5 and/or 8 ring positions. B. Preparation of the Compounds of the Invention General methods for preparing some 3-amino derivatives are found in the above-referenced patents to Ley et al., for example US 3,980,779. The compounds are prepared from benzofuroxan of the formula: by reaction with a salt of cyanamide, followed by acidification of the reaction mixture. The benzofuroxan starting material is not symmetric with respect to its own 5 and 6 positions (which are the 6 and 7 positions of the resulting 3-amino benzotriazine oxide). Therefore, a mixture of the 6- and 7-substituted materials may result. If desired, this mixture can be separated using conventional means into individual components having a substituent in either the 6 or 7 position. The dioxide may also be prepared from the parent monoxide or 1,2,4-benzotriazine by peracid oxidation (see Robbins et al, J Chem Soc 3186 (1957) and Mason* et al, J Chem Soc B 911 (1970)). In addition, the monoxide may be prepared by (1) cyclization of a l-nitro-2-aminobenzene compound using H2NCN*2HC1; (2) oxidation of the parent compound given b the structure , or by controlled reduction of the corresponding dioxid (see Mason, supra, and Wolf et al, J Am Chem Soc 76:35 (1954)). The 1,2,4-benzotriazines may be prepared b cyclization of formazan precursors using BF3/ACOH (see Scheme I and Atallah and Nazer, Tetrahedron 38:1793 (1982)). 3-araino-l,2,4-benzotriazines may be prepared either by cyclization of a parent compound (see Scheme II and Arndt, Chem. Ber. 3522 (1913)) or by reduction the monoxide or dioxide as above. The 3-hydroxy-l,2,4-benzotriazine oxides may be prepared using peroxide and sodium tungstate (Schem III), a novel synthetic procedure for making the 3-hydroxy-l,4-dioxide compound, or concentrated sulfur acid and sodium nitrate (Scheme IV). Scheme r 15 Scheme 1 1 >SO°C o Scheme I r r Scheme rv The invention also encompasses a novel method of preparing 1,2,4-benzotriazine oxides unsubstituted at the 3 position (sometimes referred to herein as the "3-desamino" compounds). The novel synthesis involves reductive deamination of the corresponding 3-amino structure. In contrast to prior methods of synthesizing 3-desamino-l,2,4-benzotriazine oxides, the present method enables a simple, straightforward one-step method which gives the desired product in a high yield. The method involves treating a 1,2,4-benzotriazine oxide of Formula (I), wherein X is NH2, with a lower alkyl nitrite under reductive deaminating conditions. By "reductive deaminating conditions" is meant reaction conditions which will give rise to at least about 10%, preferably at least about 50%, of the desired 3-unsubstituted reaction product. A preferred lower alkyl nitrite for use in said method is t-butyl nitrite. Exemplary reductive deaminating conditions involve reaction in a compatible solvent, e.g., dimethyl formamide, at a temperature of at least about 60°C, typically at a temperature in the range of 60-65°C. This reaction is illustrated generally at Scheme V, and is exemplified in Examples 12-15 herein. C. Formulation and Administration As demonstrated below, the oxidized benzotriazines of the invention may be used to radiosensitize or selectively kill hypoxic tumor cells in warm-blooded animal hosts. A way in which they may be used is in conjunction with agents known to selectively create hypoxia in tumors. Such methods include the use of antihypertensive drugs such as hydralazine, or agents which affect the amount of oxygen carried by the blood. While these compounds will typically be used in cancer therapy of human patients, they may be used to kill hypoxic tumor cells in other warm blooded animal species such as other primates, fafrm animals such as cattle, and sports animals and pets such as horses, dogs, and cats. Hypoxia is believed to be associated with all types of solid malignant neoplasms. The compounds of the invention may, therefore, be used to radiosensitize or to kill neoplastic epithelial cells, endothelial cells, connective tissue cells, bone cells, muscle cells, nerve cells, and brain cells. Examples of carcinomas and sarcomas include carcinomas such as epithelial cell, acidic cell, alveolar cell, basal cell, basal squamous cell, cervical, renal, liver, Hurthle, Lucke, mucinous and Walker, and sarcomas such as Abernathy's, alveolar soft part, angiolithic, botyroid, encephaloid, endometria stroma, Ξwing's fascicular, giant cell, lymphatic, Jensen's, juxtacortical osteogenic, Kaposi's, medullary, and synovial. Specific examples of tumors that have been sensitized with other radiosensitizers are reported in Adams, G.E., Cancer: A Comprehensive Treatise (F. Becker, Ed) vol 6, pp 181-223, Plenum, New York, 1977. The compounds may be administered to patients orally or parenterally (intravenously, subcutaneously, intramuscularly, intraspinally, intraperitoneally, and. the like). When administered parenterally the compounds will normally be formulated in a unit dosage injectable form (solution, suspension, emulsion) with a pharmaceutically acceptable vehicle. Such vehicles are typically nontoxic and nontherapeutic. Examples of such vehicles are water, aqueous vehicles such as saline, Ringer's solution, dextrose solution, and Hank's solution and nonaqueous vehicles such as fixed oils (e.g., corn, cottonseed, peanut, and sesame), ethyl oleate, and isopropyl myristate. Sterile saline is a preferred vehicle and the compounds are sufficiently water soluble to provide a solution for all foreseeable needs. The vehicle may contain minor amounts of additives such as substances that enhance solubility, isotonicity, and chemical stability, e.g., antioxidants, buffers, and preservatives. When administered orally (or rectally) the compounds will usually be formulated into a unit dosage form such as a tablet, capsule, suppository or cachet. Such formulations typically include a solid, semisolid or liquid carrier or diluent. Exemplary diluents and vehicles are lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, mineral oil, cocoa butter, oil of theobroma, alginates, tragacanth, gelatin, methylcellulose, polyoxyethylene sorbitan monolaurate, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, and magnesium stearate. The amount of compound administered to the subject is sufficient to radiosensitize or to produce cytotoxicity in the malignant neoplasm to be treated but below that which may elicit toxic effects to normal tissue. This amount will depend upon the type of tumor,, the species of the subject being treated, the indicated dosage intended and the weight or body surface of the subject. The radiation may be administered to humans in a variety of different fractionation regimes, i.e., the total radiation dose is given in portions over a period of several days to several weeks. These are most likely to vary from daily (i.e., five times per week) doses for up to six weeks, to once weekly doses for four to six weeks. An individual dose of the benzotriazine will be given before or after each radiation treatment and is likely to be in the range of 0.01 to 20 mmol/kg and usually in the range of 0.1 to 2 mmol/kg. In these treatment regimens, each radiation dose is typically 1-5 Gy, preferably, less than 2.5 Gy and more preferably 2-2.5Gy. Typically, one dose of radiation is administered per day although 2 or more might be used if tolerated by the patient It has now been found that the compounds disclosed herein as radiosensitizers, particularly 3-amino-l,2,4-benzotriazine 1,4-dioxide, both sensi ize tumors to radiation without increasing the sensitivity of normal skin, and work in highly fractionated radiation regimens. As demonstrated in Example 22 herein, pre- or post-irradiation treatment of cells with, for example, 3-amino-l,2,4-benzotriazine 1,4-dioxide, under hypoxic conditions, radiosensitizes cells even when the drug is not present during the radiation exposure and the cells are aerobic. For use as selective cytotoxic agents, the compounds of the invention can be administered alone, with radiation or other cancer cytotoxic agents, with vasoactive drugs (e.g., hydralazine), or under other conditions which reduce the amount of available oxygen carried by the blood such as anemia or drugs which increase the binding of oxygen to hemoglobin, all of which can enhance selectively the degree of hypoxia in the tumor. Examples The following examples further illustrate the compounds of the invention and methods for synthesizing and using them, and are not intended to limit the invention in any manner. Experimental: All reactions were carried out in flame-dried glassware and under a blanket of Argon, t-butyl nitrite (90%) was purchased from the Aldrich Chemical Company. Dimethylformamide was distilled from calcium hydride. 7-Nitro-3-amino-l,2,4-benzotriazine- 3-amine 1-oxide was purchased from Parish Chemical Company, trifluoroacetic anhydride, N,N-diethylethylene- diamine, N,N-diethylpropylenediamine and sodium . tungstate dihydrate were purchased from Aldrich Chemical Company and 70% hydrogen peroxide was a gift from Interox America. All reactants were used without further purification. Flash chromatography was carried out on E. Merck 230-400 mesh silica gel under a positive pressure of argon. NMR spectra were obtained on a Varian XL-400 or Jeol FX90Q spectrometer and in d6~acetone, d4~methanol, or dg-dimethyl sulfoxide, as indicated, and are reported relative to the central peak in the appropriate multiplet (2.04, 3.30, and 2.49 ppm, respectively), UV spectra were obtained on a Perkin-Elmer 552 spectrophotometer in 95% ethanol, mass spectra were obtained on an LKB 9000 mass spectrometer, and elemental analyses were carried out by Desert Analytics, Tucson, AZ. Example 1: Preparation of 3-Hvdroxy-1.2,4-Benzotriazine 1,4-Dioxide A stirred mixture of 1.50g (9.25 mmole) of 3-amino-l,2,4-benzotriazine 1-oxide ( I ) , 100.0 ml acetic acid, and 30.0 ml of 30% hydrogen peroxide was treated with 3.05 g (9.25 mmole) of Na2Wθ 2H20. The mixture was stirred in an oil bath at 60 β C for 4 days. The yellowish orange mixture was cooled to about 30° and filtered to remove a light yellow non-UV absorbing solid. The orange solution of hydrogen peroxide in acetic acid was evaporated to semi-dryness carefully with several additions of water and acetic acid to remove most of the peroxide. The concentrated solution was allowed to stand at room temperature to afford four crops of an orange solid, 0.87g (42% yield of the sodium salt of 2). UV max (20% CH3OH/H2O): 262.2 (ε 39,460); 477 (e 7,030). IR (neat): 3530μ, 3150μ, 2650μ, 2180μ and 1635μ. Anal, (calculated for the sodium salt): C7H4N3θ3Na 1.25H20, 223.64: C,37.6; H,2.93; N, 18.79. Fcund: C, 37.8; H,2.75; N,18.65. Example 2: Preparation of 3-Amino-7-Trifluoromethyl- 1,2.4-Benzotriazine 1-Oxide: A mixture of 4-chloro-3-nitrobenzotrifluoride (Aldrich, 2.70 g, 12.9 mmole) and cyanamide dihydrochloride (2.75 g, 24 mmole) (previously prepared by treating an ether solution of cyanamide with HCl gas and collecting the precipitated solid) was heated at 140°C for 1 h. The residue was treated with 2N NaOH (4 ml), heated for a further 5 min, and then allowed to cool. The precipitate was collected, washed with H2O, dried, and triturated with acetone-toluene to yield 1.3 g (45%) of 2 as a light yellow solid M.P. 301-302°, TLC: Rf 0.60 (9:1 methylene chloride: methanol on silica gel plates). Mass. Spec: M + =230 (q - 100). Example 3: Preparation of 3-Amino-7-Decyl-l,2,4- Benzotriazine 1-Oxide Preparation of 4-(l-decyl)-2-nitroaniline: Acetic anhydride (400 ml) was added over a 30-minute period to a stirred solution of 4-decylaniline (Aldrich, 80g, 0.34 mole) in hexanes (2.41). After stirring for lh, the mixture was cooled and treated over 30 min. at 5-10°C with 70% nitric acid (34 ml). Stirring was continued at 5-10°C for lh and at 25°C for 16h. The mixture was diluted with H2O (11), stirred for 5h, poured into an open dish and allowed to stand for 16h. After further dilution with H2O (1.51), the solid was collected and recrystallized from an 85% ethanol solution (in water) to give 92g (84%) of the intermediate as an orange solid, m.p. 64°C. A solution (100 ml) of 85% KOH (19g, 0.288 mole) in H2O was combined with a suspension of 4-(l-decyl)-2-nitroaniline (89g, 0.28 mole), prepared above, in methanol (900 ml). The mixture was stirred for 6h, neutralized to pH 7-8 with concentrated HCl, a evaporated in vacuo to near dryness. After dilution with H2O (400 ml), the solid was collected and air-dri to give 77g (100%) of the intermediate as an orange solid, m.p. 59°C. l.Og (8.7 mmole) of cyanamide dihydrochlorid (previously prepared for use by treating an ether t solution of cyanamide with HCl gas and collecting thef precipitated solid) was added portionwise over 10 min a preheated melt (190°C) of 4-(l-decyl)-2-nitroaniline prepared in the preceding step (500 mg, 1.8 mmole). T reaction mixture was heated at 190°C for 5 min, cooled to 25°C, treated with 6N KOH (10 ml), and heated at 90-95°C for lh. After cooling to 25°C, the solid was collected, washed with H2O and ethanol and air-dried t give 0.25g (46%) of compound 4 as a light yellow solid, m.p. r77 β C (dec). Mass. spec. M + =285 (q=100), 302 (q=13). Example 4: Preparation of 3-Amino-7-Carbamyl-l.2,4- Benzotriazine 1-Qxide Preparation of 4-chloro-3-nitrobenzamide: 20.2g (0.1 mole) of 4-chloro-3-nitrobenzoic acid (Aldrich) and thionyl chloride (20 ml) were combined, allowed to stand for 16h, and refluxed for 4h to give clear red solution. The solution was evaporated in vacuo and azeotroped with benzene. The residue was dissolved in acetonitrile (20 ml) and added over 30 mi to cold (-10°C) concentrated ammonium hydroxide (100 ml). After 3h at -10°C and 16h at 25°C the mixture wa poured into an open dish and allowed to evaporate to dryness. The residue was slurried in H2O and the soli was collected and air-dried to give 19.8g (98%) of th intermediate as a light yellow solid, m.p. 153°C. A solution of Na (3.45g, 0.15 mole ) in ethanol (75 ml) was added to a solution of guanidine hydrochloride (15.8g, 0.165 mole) in ethanol (75 ml). After lh the mixture was filtered and the filtrate was combined with a suspension of 4-chloro-3-nitrobenzamide (lOg, 0.05 mole) prepared above, in ethanol (50 ml). Th mixture was stirred and refluxed for 16h, cooled to 0-5°C, and acidified with concentrated HCl (8 ml). The collected solid was combined with K2CO3 (28g, 0.2 mole) and H2O (40 ml) and the mixture was stirred and heated at 100 β C for 8h. After cooling to 25°C, the solid was collected, washed with H2O, and air-dried. The solid was suspended in boiling ethyl acetate, collected and washed with hot ethyl acetate. The solid was repeatedl suspended in boiling dioxane and collected (6x100ml). The combined filtrate was evaporated in vacuo to a solid. The solid was suspended in 95% ethanol, collected and air-dried to give 0.44g (4.3%) of compoun 5 as a light yellow solid, m.p. 300°C. TLC: Rf=0.23 (methylene chloride: acetone of 2:1, silica gel plates) Mass. Spec: M + 205 (q= 100). Example 5: Preparation of 7-Acetyl-3-Amino-l ,2,4- Benzotriazine 1-Oxide Oxime A combined mixture of 7-acetyl-3-amino- 1,2,4-benzotriazine 1-oxide (prepared in Example 5; 50 mg, 0.25 mmole), hydroxylamine hydrochloride (200 m 2.88 mmole), pyridine (1 ml) , and ethanol (1 ml) was heated at 90-95 β C for lh and then cooled to 25°C. The mixture was diluted with 95% ethanol (5 ml) and the solid was collected and air-dried to give 30 mg (56%) compound 6 as a light yellow solid, m.p. 278°C (dec). TLC: Rf*0.60 (9:1 methylene chloride: methanol). Mas Spec: M + «219 (q=100). Example 6: Preparation of 3-Amino-6(7)-Decyl-l.2.4- Benzotriazine 1,4-Dioxide 5-(1-decyl)-benzofuroxan: A combined mixture of 4-(l-decyl)-2-nitroaniline (77g, 0.28 mole), 5.25% NaOCl in H2O (476g, 0.34 mole), 85% KOH (20.3g, 0.31 mole), nBu4NHS04 (4.7g, 0.014 mole), and CH2CI2 (2.28 1) was stirred rapidly for 6h and diluted with H2O (500 ml) and CH2C 2 (1 1). The separated organic phase was washed successively with IN HCl (1 1) and brine (2 x 1 1)), dried (Na2≤θ4), and concentrated in vacuo to yield a red oil, 70 g (92%) . A solution of 5-(1-decyl)-benzofuroxan as prepared above (10 g, 0.036 mole) and benzyltriethyl ammonium chloride (0.36 g, 0.0016 mole) in DMSO (180 ml) was treated gradually over several hours with cyanamide (13.0 g, 0.31 mole) and K2CO3 (36.8 g, 0.27 mole). The mixt re was stirred for 48h and filtered. The filtrate was diluted with H2O (6 1) and glacial acetic acid (40 ml) and extracted-w-ith CH2CI2 (4 x 500 ml). The combined organic solution was washed successively with 5% NaHCθ3 solution (1 x 500 ml) and brine (2 x 500 ml), dried (Na2≤θ4), and evaporated in vacuo to dryness. The crude product was purified by chromatography on silica gel using CH2CI2: methanol (98:2) to give 1.8g (16%) of compound 2 as a red solid, m.p. 155°C (dec). Mass. Spec: M + -318 (q«4), 285 (q=100). Example 7: Preparation of 1,2,4-Benzotriazine 1.4-Dioxide 8 A mixture of 1.80 g (13.73 mmole) of 8., 90% H2O2 (9 ml), trifluoroacetic anhydride (13.5 ml) and Na2 θ .2H2θ (12.50g, 38 mmole) in CHCI3 (170 ml) was stirred at room temperature for 5 days. The reaction mixture was diluted with H2O (100 ml) and extracted with CHCI3 (100 ml). The organic layer was washed with H2O (50 ml), dried (Na2S04), and the solvent removed in vacuo. The residue was chromatographed on silica gel using EtOAc-CH2Cl2 (1:1) to give 0.30 g ( 13.4%) of compound 9 as a yellow solid, m.p. 204-205 β C. Anal. Calc'dV for C7H5N3O2 (163.13): C, 51.5; H, 3.09; N, 25.76.*. Found: C, 51.6; H, 3.36; N, 26.01. Mass Spec. M + »163 (q»100), 147 (q«50). TLC: Rf=0.27 (EtOAc-CH2Cl2, 1:1, silica gel plates). IR (nujol): 1600μ, 1460μ, 1300μ, 1230μ. UV max (H2O) : 227 (ε 22,900) 252 (ε 12,950); 392 (ε 4,080). Example 8: Preparation of 7-Chloro-3-Hvdroxy-l,2,4- Benzotriazine 1,4-Dioxide 11 12 A mixture of 1.50 g (7.63 mmole) of .10. in 100 ml acetic acid was treated with 2.52 g (7.63 mmole) of Na2Wθ » 2H2θ and 30 ml of 30% H2O2. The mixture was stirred and heated for 6 days at 50 β C, then slowly evaporated to dryness to remove H2O2. The residue was boiled in 250 ml H2O and filtered to remove about 25 mg of starting material 12. The aqueous solutions were then extracted with 2 x 250 ml portions of ethyl acetate. A deep red crystalline material that was characterized as 12 , by TLC and Mass. Spec, analysis formed in the partitioning mixture above and was collected by filtration to afford 60.0 mg of a yellowish orange solid (3.7% yield), characterized as follows as 12. which showed good solubility in a mixture of hot isopropyl alcohol and water. Mass. Spec: M + =212 (σ=100) (compound 10): TLC: Rf= 0.34 (acetone, silica gel plates) . The ethyl acetate solutions above, separated from the H2O layer after the filtration to remove L2, were evaporated to dryness. The residue was then treated with isopropyl alcohol at room temperature to afford a dull orange solid, 0.41g (25% yield) of LI. Mass. Spec: M + =213 (q=70); TLC: Rf=0.22 (acetone, silica gel plates). Compound IJ, was characterized as the ammonium salt, C7H4ClN3θ3*NH3, m.w. 230.61, as follows. The free acid ϋ was dissolved in concentrated NH4OH and then chilled in ice and filtered to remove a trace of insoluble 1J2. The red filtrate and washings were evaporated to dryness, leaving a reddish-orange solid. The solid was treated with 50 ml of boiling 1,2-dimethoxyethane, collected on a filter and washed with an additional 25 ml of hot 1,2-dimethyl ether. The solid was dried over P2O5 at 56°C/1.0 mm, leaving 0.244 • g (87% yield) of 13 o r 13 Anal. Calc'd. for C7H4CIN3O3 NH3 (230.61): C, 36.5; H, 3.06; N, 24.30. Found: C, 36.5; H, 3.07; N, 23.94. U ax (H2O): 219 (ε 12,580); 265.4 (ε 40,000); 4830486 (ε 6^640). Example 9: Preparation of 7-Nitro-3-Amino-1.2,4- Benzotriazine 1.4-Dioxide IS \(o 7-Nitro-3-trifluoroacetamido-1,2,4- benzotriazine 1-oxide (1J5) : A solution of 7-nitro-3- amino-1,2,4-benzotriazine 1-oxide ( 1Λ ) (4.00g, 19.3 mmol; Parish Chemical Co.), CHCI3 (125 ml) and trifluoroacetic anhydride (12.0 ml, 85.0 mmol) was stirred at room temperature for 44 hr. The resultant light yellow solid was filtered, washed with CHCI3 (50 ml) nd dried to give 5.35g (91% yield) of the product as a yellow solid. Anal. Calc'd. for C9H4F3N5O4: C, 35.7; H, 1.33; N, 23.10. Found: C, 35.7; H, 1.23; N, 23.06. 7-Nitro-3-amino-l,2,4-benzotriazine 1,4-oxide (! ) : To a stirred solution of 7-nitro-3- trifluoroacetamido-l,2,4-benzotriazine 1-oxide prepared above (15.) (2.50g, 8.25 mmol) in CHCI3 (200 ml) was added Na2Wθ .2H2θ (90 mg, 0.273 mmol) followed by 70% H2O2 (10 ml). After 15 min the solution was treated • with trifluoroacetic anhydride (8.0 ml, 56.7 mmol) and stirring was continued at room temperature for 64 hr. The reaction mixture was chromatographed (EtOAc, 20% MeOH/acetone, and finally 20% DMF/acetone) then recrystallized in acetone to give 1.20g (65% yield) of the product (ljS) as an orange solid, mp 286-288°C (dec). UV: λ 259, 300, 345, 387, 472. Anal. Calc'd. for C7H5N5O4: C, 37.70; H, 2.26; N, 31.39. Found: C, 37.70; H, 2.13? N, 30.94. Example 10: Preparation of 3-(3-N,N-Diethylaminopropylamino)-1.2,4-Benzotriazine 1,4-Dioxide l~ 3-(3-N,N-diethylaminopropylamino)-1,2,4- benzotriazine 1-oxide (18): A solution of 3-chloro- 1,2,4-benzotriazine 1-oxide (1/7) (3.0g, 16.5 mmol) (produced by the method of Sasse et al., U.S. Patent 4,289,771) in CH2CI2 (100 ml) was treated with N,N-diethylpropylenediamine (9.5 ml, 88.3 mmol). After 20 hr at room temperature the mixture was diluted with 1,2-dichloroethane (50 ml) and washed successively with Na2Cθ3 and H2O. The yellow solution was dried (Na2S0 ), filtered and evaporated .in vacuo to give 3.93g (87% yield) of the product as a yellow solid. Recrystallization (ether/petroleum ether) yielded pure material, mp 47-48°C. Anal Calc'd. for C14H21N5O (18): C, 61.10; H, 7.69; N, 25.44. Found: C, 61.30; H, 7.8e; N, 25.61. 3-(3-N,N-diethylaminopropylamino)-1,2,4- benzotriazine 1,4-dioxide (18a): To a stirred solution of 3-(3-N,N-diethylaminopropylamino)-l,2,4-benzotriazine 1-oxide JL8 prepared as above (1.60g, 6.10 mmol) in CHCI3 (50 ml) was added trifluoroacetic anhydride (22.0 ml). After 15 min the mixture was cooled to -10°C, 70% H2O2 (10 ml) added and then stirred at room temperature for 20 days. The reaction mixture was dried (Na2S0 ), filtered and evaporated to dryness. The residue was dissolved in saturated NaHCθ3 solution (50 ml) and extracted with CH2CI2 (3x150 ml). The organic layer was dried ( a2S04), filtered and evaporated to give the product 18a, 0.51g (29% yield) as a red solid, mp 92-9 °C. NMR: δ (400 MHz, CDCI3) 1.11 (6H, t, J=7.1 Hz, CH3), 1.84-1.90 (2H, m, H-2'), 2.48-2.64 (4H, m, NCH2CH3 and H-3'), 3.68 (2H, br t, J*5.5 Hz, H-l' ) , 7.46 (IH, ddd, J-7.1, 7.0 and 1.2 Hz, H-6), 7.84, ddd, J-7.0, 6.9 and 1.2 Hz, H-7), 8.31 (2H, m, H-5 and 8), 8.80 (IH, br s, NH). UV: λ 220, 270, 476. Anal. Calc'd. for C1 H21N5O2. (1/3 H2O): C, 56.50; H, 7.34; N, 23.55. Found: C, 56.90; H, 7.15; N, 23.40. Example 11: Preparation of 7-Nitro-3-(2-N.N- Diethylaminoethylamino)-1.2,4-Benzotriazine 1,4 Dioxide I 4 . -10 At 7-nitro-3-(2-N,N-diethylaminoethylamino)- 1,2,4-benzotriazine 1-oxide hydrochloride (.20): A solution of 7-nitro-3-chloro-l,2,4-benzotriazine 1-oxid (19) (1.60g, 7.06 mmol) (prepared as generally shown in Sasse et al., U.S. Patent 4,289,771, with (a) NaN02 and H2SO4 at 40°C, followed by (b) chlorination with POCI3 at 106°C) in CH2CI2 (50 ml) was treated with N,N-diethylethylenediamine (6.0 ml, 42.7 mmol). After 16 hr at room temperature the mixture was evaporated to dryness under high vacuum at 60 β C. The yellow solid was stirred in 20% iPrOH/ether (150 ml) for 5 hr, filtered, washed with iPrOH then petroleum ether and dried (80°C/1.0 mmHg) to give 1.80g (74% yield) of the product 20 as yellow needle crystals. NMR δ (90 MHz, dg-DMSOΛU-MeOH) 1.25 (6H, t, J=6.0 Hz, CH3), 3.25 (6Hj, m, NCH2), 3.82 (2H, m, H-l'), 7.74 (IH, d, J=7.0 Hz, H-5), 8.52 (IH, dd, J=7.0 and 2.0 Hz, H-6), 8.91 (IH, d, J=2.0 Hz, H-8). 7-nitro-3-(2-N,N-diethylaminoethylamino)- 1,2,4-benzotriazine 1,4-dioxide hydrochloride (2JL.) . To a stirred solution of 7-nitro-3-(2-N,N-diethylamino- ethylamino)-l,2,4-benzotriazine 1-oxide hydrochloride (20; prepared as described above) (0.50g, 1.46 mmol) in CHCI3 (50 ml) at 0°C was added trifluoroacetic anhydride (9.0 ml). After 30 min 70% H2O2 (4.0 ml) was added and the mixture stirred at room temperature for 3 days, then dried (Na2Sθ4), filtered, and evaporated in vacuo to dryness to give the trifluoroacetate salt 0.67g (45% yield). This product was dissolved in saturated NaHC03 solution (30 ml) and extracted with CH2CI2 (3x30 ml). The dichloromethane was washed with H2O, dried (Na2Sθ ), filtered, saturated with gaseous HCl and evaporated to dryness to give 0.35g (63% yield, 28% overall) of the product as a red solid, m.p. 194-195°C. UV: λ 260, 306, 388, 479. Anal. Calc'd. for C13H18N6O4.HCI: C, 43.50; H, 5.34; N, 23.43. Found: C, 43.20; H, 5.37; N, 23.11, The following Examples 12-15 are directed to reductive deamination reactions for preparing compounds of Formula (I) which are unsubstituted at the 3-position, i.e., wherein the substituent "X" is hydrogen. Example 12: Preparation of 1,2.4-Benzotriazine 1.4- Dioxide by Reductive Deamination of 3-Amino-l,2,4- Benzotriazine 1,4-Dioxide 22 To a rapidly stirred solution of t-butyl nitrite (867 mg, 1.0 ml, 8.41 mmol) in DMF (20 ml) at 60-65 β C was added 3-amino-l,2,4-benzotriazine 1,4-dioxide ("SR 4233") (500 mg, 2.81 mmol) (prepared by the method of Seng et al., Angew. Chem. Internat. Edit. 11:11 (1972)) in small portions over 5 min. Following the addition, and subsidence of the concomitant effervescence (approx. 5 min), the solution was cooled and reduced under high vacuum to a dark waxy solid. Flash .chromatography (30% EtOAc/CH2 c l2) gave a yellow solid, mp 188-189.5°C (dec), which was recrystallized from methanol to give 195 mg (43% yield) of the product 9 as bright yellow platelets, mp 192-194°C (dec). NMR: • δ (400 MHz, d6-acetone) 8.04 (1 H, ddd, J»8.5, 7, 1.5 Hz), 8.15 (1 H, ddd, J=8.5, 7, 1.5 Hz), 8.42 (1 H, dd, J=8.5, 1.5 Hz), 8.43 (1 H, dd, J=8.5, 1.5 Hz) 9.05 (1 H, s, H-3). UV: λ 405, 300, 225. MS, m/z (relative intensity) 164(9), 163(100, M + ), 147(13), 136(19), 90(7), 78(27), 76(26), 75(8), 64(9), 63(10), 52(12), 51(48), 50(28), 38(8), 37(5), 30(18), 28(6), 27(7). Anal. Calc'd. for C7H5N3O2: C, 51.54; H, 3.09; N, 25.76. Found: C, 51.42; H, 3.02; N, 25.66. Example 13: Preparation of 7-Allyloχy-l,2.4- Benzotriazine 1.4-Dioxide Via Reductive Deamination 23 2. 7-Allyloxy-l,2,4-benzotriazine 1,4-dioxide 2Λ \ To * stirred solution of t-butyl nitrite (271 mg, 0.312 ml, 2.63 mmol) in DMF (15 ml) at 60-65°C was added 7-allyloxy-3-amino-l,2,4-benzotriazine- 1,4-dioxide 2. (205 mg, 0.875 mmol) in small portions over 5 min. After 30 min additional t-butyl nitrite (271 mg, 0.312 ml, 2.63 mmol) was added, and shortly thereafter the deep red solution effervesced and lightened appreciably in color over a period of a few minutes. After an additional 30 min the resultant orange solution was reduced under vacuum to a brown solid which was sequentially flash chromatographed (10% EtOAc/CH2Cl2. and crystallized (CH2Cl2/petroleum ether) to give 72 mg (38% yield) of the product .24 as light orange crystals, mp 147-148°C. NMR: δ (400 MHz, dβ-acetone) 4.89 (2 H, ddd, H-l', Jι ι f 2'"5.5 f Jl' ,3'cis'^l' ,3'trans'l* 5 Hz ^ 5.36 (1 H, ddd, H-3', J3' ,2'cis»10.5, J3»,3'»3, J3» r l«-1.5 Hz), 5.52 (1 H, ddd, H-3 « , J 3 « , 2 'trans e 17.5, J3',3' s 3, J3»ι»»1.5 Hz), 6.14 (1 H, ddt, H-2' , J2' ,3'cis s 10 ' 5, J2'_i'-5.5 Hz), 7.70 (1 H, d, H-8, J 8 ,6 β 2.5 Hz), 7.74 (1 H, dd, H-6, J 6 ,5=9.5, J6,8=2.5 Hz), 8.33 (1 H, d, H-5, J5,6 a 9« 5 Hz >, 8.93 (1 H, s, H-3). UV: λ 425, 410, 365, 355, 320, 245, 200. MS m/z/ (relative intensity) 220(4), 219(34,M + ), 103(4), 77(4), 75(4), 63(13), 62(4), 42(3), 41(100), 39(16). Anal. Calc'd. for C10H9N3O3: C, 54.79; H, 4.14; N, 19.17. Found: C, 54.73; H, 4.16; N. 19.15. Example 14: Preparation of 7-(3-N-Ethylacetamido-2- acetoxypropoxy)-1.2.4-Benzotriazine 1,4-Dioxide Via Reductive Amination 25 26 To a stirred solution of t-butyl nitrite (185 mg, 1.79 mmol) in DMF (5 ml) at 60 β C was added via syringe a solution of 7-(3-N-ethylacetamido-2-acetoxypropoxy)-3-amino- 1,2,4-benzotriazine 1,4-dioxide (.25.) (125 mg, 0.329 mmol) in DMF (5 ml) over a period of 1 min. After 5 min additional t-butyl nitrite (217 mg, 2.10 mmol) was added and an immediate reaction occurred, as evidenced by the evolution of a gas and a change in color of the solution from red to light orange. After an additional 10 min the solution was stripped to a yellow/brown solid and eluted through silica gel with 5% MeOH/CH2Cl2 to give 119 mg of a yellow oil. Recrystallization from CH2Cl2/ligroin gave 90 mg yellow solid (75% yield), mp 179-180.5°C. NMR: δ (400 MHz, d4-methanol, mixture of rotamers, ratio approx. 2:1) 1.12, 1.22 (t's, 1:2, 3 H total, J=7 Hz), 2.06, 2.07 (s's, 2:1, 3 H total), 2.11, 2.17 (s's, 2:1, 3 H total), 3.41-3.92 (m, 4 H) , 4.34-4.48 (m, 2 H) , 5.48-5.58 (m, 1 H) , 7.76-7.86 (m, 2 H), 8.36-8.42 (m, 1 H) , 9.04, 9.06 (s's, 2:1, 1 H total). UV: λ 420, 405, 365, 350, 315, 240, 200. MS: m/z (relative intensity) 365(0.5), 364(1.4, M + ), 349(0.5), 348(1.1), 347(0.5), 332(1.2), 331(3.6), 187(,7), 186(66), 102(6), 100(21), 84(30), 63(6), 58(100), 56(8), 43(65), 42(9), 41(5), 30(14), 29(5), -SO- Example 15: Preparation of 7-Nitro-l,2,4-Benzotriazine 1.4-Dioxide via Reductive Deamination 14 27 To a stirred solution of t-butyl nitrite (88 mg, 0.85 mmol) in DMF (5 ml) at 60 β C was added 7-nitro-3-amino-l,2,4-benzotriazine 1,4-dioxide ( l±) (38 mg, 0.17 mmol). After 30 min the addition of further t-butyl nitrite (175 mg, 1.70 mmol) to the dark red slurry was immediately followed by a change in coloration and effervescence. After an additional 10 min the orange solution was reduced to a red solid in vacuo and chromatographed with 1% ACOH/CH2CI2 to give 3 mg of the product 27 as a yellow solid (10% yield). NMR δ (90 MHz, d6-dimethyl sulfoxide) 7.68 (d, IH, J = 9.2 Hz)/ 7.92 (dd, IH, J » 9.2, 2.2 Hz), 8.10 (d, IH, J = 2.2 fcz), 8.65 (s, IH)). UV: λ 420, 310, 240, 205. MS:m/z (relative intensity) 209 (9), 208 (100, M + ), 192 (54), 181 (14), 162 (16), 105 (9), 77 (28), 75 (52), 74 (27), 63 (21), 62 (16), 30 (77), 18 (26). Example 16: In Vivo Assay for Activity in Combination with Radiation Compounds of the invention were tested in viv for activity by the assay of Brown, J.M., Radiation Res (1975) 6_4:633-47, incorporated herein by reference. For this assay, SCCVII carcinomas in female C3H mice weighing 20-25 g were used. These mice were bred under specific pathogen-free conditions and were 3-4 months old at the beginning of each experiment. The SCCVII tumor was grown intradermally in the flank from an inoculation of 2 x 10 5 tumor cells taken from the 2nd-8th .in vitro passage of the tumor cells after removal from the previous .in vivo tumor. Two tumors pe mouse were implanted, and were used as subject tumors when they reached a volume of approximately 100 ml. At this point the tumors contained approximately 20% hypoxic cells. The test compound was tested at a fixed injected dose of either 5 mmol/kg or 2/3 of the LD50 (whichever was lower). Suitable controls of test compound-injected but nonirradiated and saline-injected and irradiated mice were also included. A fixed radiation dose of 20 Gy was applied at variable intervals of 2 hr after to 3 hr before injection of the drug * . By using these intervals, the results give an indication of both the optimum irradiation time and the extent of extra cell killing compared to radiation alone. The results of such time-course experiments using 3-amino-l,2, -benzotriazine 1,4-dioxide are shown in Figure 2. They show enhanced cell killing compared to radiation only, more than would have been expected o the basis of additivity of the two individual cytotoxicities. The similar increased cytotoxicity whe the drug is given before or after radiation indicates selective toxicity to the hypoxic cells rather than a - radiosensitizing effect of the benzotriazine dioxide. Irradiation of the SCCVII tumors was done by irradiating nonanaesthetized tumor-bearing mice in a Plexiglas box. Irradiation conditions were 250 kVp X-rays, 15 mA, FSC 33 cm, added filtration of 0.35 mm Cu, half value layer 1.3 mm Cu, and a dose rate of 317 rad/min. The amount of cell killing was judged by survival rate of dissected and cultured tumor cells as follows. The tumor-bearing mice were killed 24 hr after irradiation, and tumors were dissected from the skin, cut into several pieces, and made into a fine brei by high-speed chopping with a razor blade attached to a jigsaw. The brei was added to 30 ml of Hank's buffered salt solution (HBSS) containing 0.02% DNase, 0.05% promase, and 0.02% collagenase. The suspension was stirred for 30 rain at 37 β C, filtered, and centrifuged at 1,600 rmp for 10 min at 4°C. The cell pellet was resuspended in complete Waymouth's medium plus 15% fetal calf serum (FCS) and an aliquot mixed with trypan blue and counted with the use of a hemacytometer. Suitable dilutions of this serum plated into 60- or 100-mm polystyrene petri dishes (Lux Scientific Corp) in 5 or 15 ml of medium. After incubation for 13 days, the colonies were fixed and stained, and those containing 50 cells or more were counted. The dilution yielding an average count of—25 100 colonies in a 60 mm dish was used in calculation of results. Example 17: Cytotoxicity Tests Cytotoxicity tests were carried out using 3-amino-l,2,4-benzotriazine 1,4-dioxide and a variety of aerobic and hypoxic cells in culture (human, mouse, and hamster). The cells in spinner flasks were gassed for one hour at 37°C with either air or nitrogen containing 5% CO2 prior to adding the specified amounts of the drug. Figures 1A, IB and IC show the results for cell survival of mouse, hamster and human cells at various concentrations of 3-amino-l,2,4-benzotriazine 1,4- dioxide. It was found that only 1 to 2% of the drug concentration under aerobic conditions was required to get equal cell killing under hypoxia. This ratio of selective hypoxic toxicity (50-100) is higher than that for any compound so far reported in the literature. Example 18: Determination of LDgn LD50 is determined in BALB/c female mice (weighing 20-25 g) following intraperitoneal (ip) injection, unless the compound tested has low lipophilicity and is very soluble, wherein intravenous (iv) administration is used. LD50 values at 1, 2, 5, and 60 days are determined by administering graded doses of the drug dissolved in physiological saline immediately prior to injection. *t . . . . . Example 19: Radiosensitivity n Vitro The results of assays to determine the concentration of drug necessary to produce a sensitizer enhancement ratio of 1.6 of hypoxic cells in culture are as follows: Compound Ci, - (mM) 7-chloro-3-amino-l,2,4-benzotriazine 3.3 1-oxide 6(7)-methoxy-3-amino-l,2,4-benzotriazine 1,4-dioxide "1.0 3-hydroxy-l,2,4-benzotriazine 1,4-dioxide "2.0 Modifications of the above described modes for carrying out the invention that are apparent to those of skill in the chemical, pharmaceutical, medical, and related arts are intended to be within the scope of the following claims. Example 20: Enhanced Tumor Cell Toxicitv Using Hydralazine Hydralazine is an antihypertensive drug which acts by relaxing the smooth muscle around blood vessels. This has the effect of preferentially shunting blocd flow into normal tissues and away from tumors, which process produces immediate hypoxia in the tumors. If 3-amino-l,2,4-benzotriazine 1,4-dioxide is given in conjunction with this agent, there is a massive increase in tjumor cell killing. In this experiment, neither hydralazine nor the aforementioned benzotriazine compound produced any significant cell killing in the SCCVII tumor, whereas the combination of the two reduced survival by a factor of 10^ (i.e., only 1 cell in every 1000 was left viable). The experimental procedures are the same as described in Example 9, and the results are shown in Figure 3. Example 21: Phvsicochemical and Biological Properties of Some 1,2,4-Benzotriazine 1,4-Dioxides The following table sets forth various properties of compounds 2_2, 1± > 18a, and l as determined by the inventors herein: RHT C HCR^ LD50* a.) Log of the octanol-water partition coefficient as measured by the method of Fujita et al., J. Amer. Chem. Soc. 86_:5175 (1964), using pH 7.4 buffer. b. ) Polarographic half-wave reduction potentials measured in Britton & Robinson pH 7.4 buffer using a dropping mercury electrode. c) Relative Hypoxic Cytoxicity: Ratio of equitoxic concentrations of 22.;analog for HA-1 cells attached, under hypoxic conditions. Exponentially growing cells were placed in suspension culture and gassed for 90 min in nitrogen or air prior to addition of drugs. Samples were removed periodically for a survival determination, and the ratios determined from o a comparison of the resulting survival curves. d. ) Hypoxic Cytoxicity Ratio: Ratio of equitoxic concentrations of each analog for HA-1 cells attained under hypoxic:aerobic conditions. Treatment conditions as above. e.) Balb/c female mice 3-5 months of age were used in the LD50 experiments. LD50 was evaluated as described in Example 18. As may be readily deduced from the table, novel compounds l , lj3a,and 21. exhibit significantly enhanced cytotoxicity against hypoxic HA-1 tumor cells in vitro compared to 3-amino-l,2,4-benzotriazine 1,4-dioxide (12.), while retaining the high differential cytotoxicity against hypoxic cells compared to aerobic cells. These results suggest that these drugs will be more tumor-specific and therefore more effective as antitumor agents .irt vivo. Example 22: Fractionated Radiotherapy Using 3-Amino-l,2,4-Benzotriazine 1.4-Dioxide The following experimental work establishes that pre- or post-irradiation treatment of cells .in vitro with compound 2J2 under hypoxic conditions radiosensitizes the cells even when drug is not present during radiation exposure and the cells are aerobic. a.) Figure 4 shows the results of experiments in whith the survival of Chinese hamster ovary (CHO) cells after graded doses of x-rays was determined either with a hypoxic compound 2_2 exposure given before or after irradiation. The preirradiation treatments consisted of 20 μM drug for exposure times of 1.0 (D in Figure 4), 1.5 (<Δ ) , 2.0 (<>) and 2.5 (£7) h duration prior to cells being reaerated and irradiated. These drug treatments alone reduced cell survival to approximately 32, 19, 8 and 2% respectively. The postirradiation drug treatment consisted of 20 μM for 1.5 h (+), which alone reduced cell survival to 23%. Compared to the survival curve for cells exposed to pre- or post-irradiation hypoxia only (■ ), treatment with compound 22 sensitized cells to aerobic irradiation. The sensitization was predominantly a change in the slope of the radiation survival curve. D σ decreased from 1.34 Gy (95% confidence limits: 1.09-1.76 Gy) to 0.80 Gy (95% confidence limits: 0.73-0.88 Gy) , based on least-squares regression analysis of pooled data for the exponential portions of the survival curves for control and treated cells. However, sensitization at low radiation doses was also readily apparent. Survival ratios for "no drug:drug- treated" CHO cells irradiated with doses of 1-3 Gy averaged a factor of 3.6. The amount of the radiosensitization produced did not vary with the severity of the drug treatment. b. ) The data of section (a.) on radiosensitization of aerobic cells at low radiation doses by hypoxic activation led us to test the feasibility of obtaining preferential radiosensitization of tumors .in vivo. Our protocol was to use eight doses of 2.5 Gy/dose in four days (irradiating 2 x/day) . Because of the jj vitro data showing that radiosensitization could only be achieved by hypoxia activation, either before or after irradiation, and because we did not want to make the tumors hypoxic before irradiation (as this would make them resistant), we made the tumors hypoxic after irradiation using the vasoactive drug hydralazine (HDZ) at the same time as injecting compound .22. We used tw different types of controls. First, compound .22 , alone before each dose of radiation, and second, the potent hypoxic cell sensitizer SR 2508 (a 2-nitroimidazole, DuPont, undergoing Phase III clinical trials in Europe) before each dose. The effectiveness, or lack of effectiveness, or SR 2508 indicates whether the radiation response of the tumor is being governed by hypoxic, or aerobic, cells respectively. We assayed the efficacies of the treatments using clonogenic cell survival and also regrowth delay. Figures 5 and 6 sho the results. As Figure 5 illustrates, there was no effect of SR 2508 (1000 mg/kg) given before each radiation dose, but compound .22 (0.08 mmole/kg) given alone or with hydralazine (HDZ) produced a large enhancement of the radiation response. Part of this is attributable to an additive response (crosses), but the additional cell killing is the result of radiosensitization of aerobic cells. In Figure 6, it may be seen that SR 2508 (1000 mg/kg) before each radiation dose had no radiosensitizing effect, but compound 2J2 (0.08 mmole/kg) alone before irradiation or with HDZ after irradiation produced a large increase in effect compared to radiation or drug alone. The major and unexpected result of these experiments is the radiosensitization of the tumors by compound 22 given before each radiation dose without the addition of hydralazine. This cannot be accounted for by a radiosensitization of hypoxic cells, since SR 2508 (a hypoxic radiosensitizer) is ineffective. Thus, it is a radiosensitization of aerobic tumor cells. This would not be useful if it radiosensitized aerobic normal cells. We tested this by performing the same eight fraction protocol on the response of normal mouse skin using a skin reaction scoring scale previously used by us. Figure 7 shows the result. There is no radiosensitization of normal skin. In conclusion, the data show that the aerobic cells of tumors can be radiosensitized in multifractfon regimes similar to those used in radiotherapy. The radiosensitization is tumor specific (i.e., does not occur in normal cells), and appears to be the result of activation by hypoxic areas in the tumors.

Claims: Claims 1. A method of selectively killing hypoxic tumor cells comprising administering to said cells a pharmaceutical composition comprising a compound of the formula 0 wherein X is NH2, NHR or NRR where each R is independently an alkyl of 1-4 carbon atoms or acyl of 1-4 carbon atoms, or wherein in the case of NRR the two R groups may be linked together to form a morpholino, pyrrolidino or piperidino ring, and wherein R may be further substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substituents; n is 1; and Y 1 and Y 2 are independently either H; nitro; halo§en; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y 1 and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R' , NH(CO)R' , 0(S0)R' , or 0(POR' )R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl-(l-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents, or a pharmacologically acceptable salt of said compound. 2. The method of claim 1, wherein X is NH2. 3. The method of claim 2, wherein Y 1 and Y 2 are both H. 4. The method of claim 2, wherein Y 1 is H and Y 2 is nitro. 5. The method of claim 1, wherein X is -NH-nCH2- ( CH2-m~CH2-NRιR2 wherein m is an integer in the range of 0-4 inclusive, and Ri and R2 are independently selected from hydrogen or lower alkyls or together form a piperidino or pyrrolidino ring. 6. The method of claim 5, wherein n is 1 or 2 and Y 1 and Y 2 are independently selected from the group consisting of H and nitro. 7. A method of selectively killing hypoxic tumor cells comprising administering to said cells a pharmaceutical composition comprising a compound of the formula wherein X is H or hydrocarbyl (1-4C), and, if hydrocarbyl, may be substituted with OH, NH2, alkoxy (1-4C), or halogen substituents; n is 1; and Y 1 and Y 2 are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or pipeζidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y~ and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(C0)R' , NH(CO)R' , 0(SO)R' , or 0(POR')R' in which R* is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl-(l-4C) secondary amino f dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; or a pharmacologically acceptable salt of said compound. 8. The method of claim 7, wherein X is H. 9. The method of claim 7, wherein X is hydrocarbyl (1-4C). 10. The method of claim 7, wherein Y~ and Y 2 are both H. 11. The method of claim 8, wherein Y 1 and Y 2 are both H. 12. The method of claim 9, wherein Y~ and Y 2 are both H. 13. A method of radiosensitizing hypoxic tumor cells comprising administering to said cells a pharmaceutical composition comprising a compound of the formfila: wherein X is halogen; OH; alkoxy (1-4C); NH2; NHR or NRR, wherein the R groups are independently selected from alkyl (1-4C) and acyl (1-4C) and the R's may themselves be substituted with OH, NH2, lower alky 1 ! (1-4C) secondary and dialkyl (1-4C) tertiary amino groups, alkoxy (1-4C) or halogen, and in the case of NRR, the two R's can be linked together directly or through a bridge oxygen into a morpholino ring, pyrrolidino ring or piperidino ring; wherein n is 0 or 1; and Y~ and Y are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y 1 and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R' , NH(CO)R* , 0(S0)R' , or 0(P0R')R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl-(l-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; or a pharmacologically acceptable salt of said compound. 14. The method of claim 13, wherein X is OH or OR. 15. The method of claim 13, wherein X is NH2, NHR or NRR. 16. The method of claim 15, wherein X is NH2. 17. The method of claim 14, wherein Y 1 and Y 2 are H. 18. The method of claim 15, wherein Y ~ and Y 2 are H. 19. The method of claim 16, wherein Y 1 is H, Y ~ is nitro, and n is 1. 20. The method of claim 13, wherein X is -NH-CH2-(CH2)m~CH2-NRιR2 wherein m is an integer in the range of 0-4 inclusive, and Ri and R2 are independently selected from hydrogen or lower alkyls or together form a piperidino or pyrrolidino ring. 21. The method of claim 20, wherein m is 1 or 2 and Y 1 and Y 2 are independently selected from the group consisting of H and nitro. 22. A method of radiosensitizing hypoxic tumor cells, comprising administering to said cells a pharmaceutical composition comprising a compound of the formula: 0 wherein X is H; hydrocarbyl (l-4C);or hydrocarbyl (1-4C) substituted with OH, NH2; NHR or NRR, wherein the R groups are independently selected from alkyl (1-4C) and acyl (1-4C), optionally substituted with OH, NH2, alkyl (1-4C) secondary and dialkyl (1-4C) tertiary amino groups, alkoxy (1-4C) or halogen, and in the case of NRR, the two R's can be linked together directly or through a bridge oxygen into a morpholino ring, pyrrolidino ring or piperidino ring; wherein n is 0 or 1; and Y~ and Y 2 are independently either H; nitro, halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y 1 and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R' , NH(CO)R* , 0(SO)R' , or 0(P0R' )R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl-(l-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; or a pharmacologically acceptable salt of said compound. 23. The method of claim 22, wherein X is H. 24. The method of claim 22, wherein X is hydrocarbyl (1-4C). 25. The method of claim 22, wherein Y 1 and Y 2 are both H. c- 26. The method of claim 23, wherein Y~ and Y 2 are both H. 27. The method of claim 24, wherein Y 1 and Y 2 are both H. 28. A compound having the structural formula: wherein X is OH, alkoxy (1-4C), NHR or NRR where each R is independently an alkyl of 1-4 carbon atoms, or acyl of 1-4 carbon atoms, or where the two R groups are alkyls linked together to form a pyrrolidino or piperidino ring or linked through an oxygen to form a morpholino ring, and the R groups may be further substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; n is 1; and Y and Y are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (l-4θ, primary amino (NH2), lower alkyl (1-4C) secatndary amino, dialkyl (1-4C) tertiary amino, dialkyl tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y~ and Y are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R' , NH(CO)R' , 0(S0)R' , or 0(POR' )R' in which R' is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; or a pharmacologically acceptable salt thereof. 29. A compound according to claim 28, wherein X is OH or alkoxy. 30. A compound according to claim 28, wherein X is NRR. 31. A compound according to claim 28, wherein Y 1 and Y 2 are both H. 32. A compound according to claim 29, wherein Y 1 and Y 2 are both H. 33. A compound according to claim 30, wherein γl aάd Y 2 are both H. 34. A compound according to claim 28, wherein X is -NH-CH2- ( CH2 ) m~ CH 2~ NR l R 2 wherein m is an integer in the range of 0-4 inclusive, and Ri and R2 are independently selected from hydrogen or lower alkyls or together form a piperidino or pyrrolidino ring. 35. A compound according to claim 34, wherein m is 1 or 2 and Y 1 and Y 2 are independently selected from the group consisting of H and nitro. 36. A compound having the structural formula: X is NH2; n is 1; and Y 1 and Y are chosen such that one but not both may be hydrogen and one or both may independently be either nitro, saturated or unsaturated hydrocarbyl of 7-14C, or unsaturated hydrocarbyl of 2-6C, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl tertiary amino where th two alkyls are linked together to produce a morpholino pyrrδlidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, " cflkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C) , alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C) , wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y^ and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(C0)R*, NH(C0)R', 0(S0)R*, or 0(P0R' )R* in which R' is a hydrocarbyl (1-4C) which may be substituted with one or more OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; or a pharmacologically acceptable salt thereof. 37. A compound according to claim 36, wherein Y is H and Y is saturated or unsaturated hydrocarbyl of 7-14C. 38. A compound according to claim 36, wherein Y 1 is H and Y is unsaturated hydrocarbyl of 2-6C. 39. A compound according to claim 36, wherein Y 1 is H and Y 2 is nitro. 40. A compound having the structural formula: X is hydrogen or hydrocarbyl (2-4C) optionally substituted with OH, NH2, alkoxy (1-4C) or halogen substituents; n is 1; and Y and are independently either H; nitro, halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), whereiln the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y 1 and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R' , NH(C0)R' , 0(SO)R', or 0(POR')R' in which R' is a hydrocarbyl (1-4C) which may be substituted with one or more OH, NH2, alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; or a pharmacologically acceptable salt thereof. 41. A compound according to claim 40, wherein X is H. 42. A compound according to claim 40, wherein s hydrocarbyl (2-4C). 43. A compound according to claim 40, wherein Y 1 and Y 2 are both H. 44. A compound according to claim 41, wherein Y 1 and Y 2 are both H. 45. A compound according to claim 42, wherein Y 1 and Y 2 are both H. 46. A method of synthesizing a 1,2,4- benzotriazine oxide having the structure 0 wherein n is 1 and Y and Y are independently either H; nitro; halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetylaminoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkylphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y x and Y^ are independently either morpholino, pyrrolidino, piperidino, NH2, NHR', NR'R' 0(CO)R' , NH(CO)R' , 0(SO)R', or 0(POR')R* in which R' is a hydrocarbyl (1-4C) which may be substituted with one or more OH, NH2, alkyl-(l-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents, or a pharmacologically acceptable salt of said compound, said method comprising: treating a 3-amino-l,2,4-benzotriazine oxide having the structure with a lower alkyl nitrite under reductive deaminating conditions. 47. The method of claim 46, wherein said lower alkyl nitrite is t-butyl nitrite. 48. The method of claim 46, wherein said reductive deaminating conditions comprise reaction in a compatible solvent at a temperature of at least about 60°C. 49. A method of radiosensitizing tumor cells in a warm-blooded mammal, comprising: (a) administering to said mammal a pharmaceutical composition comprising a 1,2,4-benzotriazine oxide having the structure wherein X is H; hydrocarbyl (1-4C); hydrocarbyl (1-4C) substituted with OH, NH2, NHR or NRR; halogen; OH; alkoxy (1-4C); NH2; NHR or NRR, wherein the R groups are independently selected from alkyl (1-4C) and acyl (1-4C), optionally substituted with OH, NH2, alkyl (1-4C) secondary and dialkyl (1-4C) tertiary amino groups, alkoxy (1-4C) or halogen, and in the case of NRR, the two R's can be linked together directly or through a bridge oxygen into a morpholino ring, pyrrolidino ring or piperidino ring; n is 0 or 1; and Y A and Y 4 are independently either H; nitro, halogen; hydrocarbyl (1-14C) including cyclic and unsaturated hydrocarbyl, optionally substituted with 1 or 2 substituents selected from the group consisting of halogen, hydroxy, epoxy, alkoxy (1-4C), alkylthio (1-4C), primary amino (NH2), lower alkyl (1-4C) secondary amino, dialkyl (1-4C) tertiary amino, dialkyl (1-4C) tertiary amino where the two alkyls are linked together to produce_a morpholino, pyrrolidino or piperidino, acyloxy (1-4C), acylamido (1-4C) and thio analogs thereof, acetyla inoalkyl (1-4C), carboxy, alkoxycarbonyl (1-4C), carbamyl, alkylcarbamyl (1-4C), alkylsulfonyl (1-4C) or alkj Iphosphonyl (1-4C), wherein the hydrocarbyl can optionally be interrupted by a single ether (-0-) linkage; or wherein Y 1 and Y 2 are independently either morpholino, pyrrolidino, piperidino, NH2, NHR*, NR'R' 0(C0)R* , NH(C0)R* , 0(S0)R' , or 0(P0R' )R' in which R* is a hydrocarbyl (1-4C) which may be substituted with OH, NH2, alkyl-(l-4C) secondary amino, dialkyl (1-4C) tertiary amino, morpholino, pyrrolidino, piperidino, alkoxy (1-4C), or halogen substitutents; and (b) subjecting said tumor cells to distinct radiation doses; and (c) repeating steps (a) and (b) such that tha mammal receives a plurality of doses of drug and radiation over an extended period of time, wherein each of said radiation doses is less than about 5 Gy. 50. The method of claim 49, wherein step (a) is carried out prior to step (b) . 51. The method of claim 49, wherein step (a) is carried out after step (b) . 52. The method of claim 49, wherein each of said radiation doses is less than about 2.5 Gy, and said extended period of time is at least about 3 days. «t 53. The method of claim 49, wherein said 1,2,4-benzotriazine oxide is 3-amino-l,2,4- benzotriazine-1,4-dioxide.

Inventors: BROWN J MARTIN, GRANGE EDWARD W, LEE WILLIAM W, MARTINEZ ABELARDO P, POLLART DANIEL J, TRACY MICHAEL, BROWN, J., MARTIN, GRANGE, EDWARD, W., LEE, WILLIAM, W., MARTINEZ, ABELARDO, P., POLLART, DANIEL, J., TRACY, MICHAEL
Applicants: STANFORD RES INST INT, SRI INTERNATIONAL
Application year: 1989
Main classification: A61K31
IPC codes: A61K31, A61K41, A61P35, C07D253