SUBSTITUTED 6H-PYRIDO (4,3-b) CARBAZOLES

Abstract: New and useful compounds which possess outstanding antitumor activity in mice and cell cultures and are active against schistosomiasis these compounds having structural formula (I), wherein R is selected from H, lower alkoxy, OH and phenoxy; R' is selected from H and CH3; R'' is selected from CH3 and CR1HOOCNHR2; R''' is selected from H and CR1HOOCNHR2; R', R'', and R''' are different from each other; when R' is CH3, R is other than H; R1 is selected from H and lower alkyl; and R2 is selected from lower alkyl, aminoalkyl and phenyl; and their pharmaceutically acceptable salts.

Description: STJBSTITUTED 6H-PYRID0 [4,3-b] CARBAZOLES Statement of Government Interest This invention resulted from research sponsored by the National Cancer Institute under grant no. R01—CA19674. The government has an interest in this application. Field and Background of the Invention The present invention relates in general to ellipticine and olivacine-like chemicals which have been shown to have schistosomicidal and antitumor effects, and, in particular, to certain substituted 6H-pyrido [4,3-b] carbazole N-alkyl or phenyl carbamates . A particularly pertinent reference in connection with this invention is Archer, S.; Ross, B. S.; Pica-Mattoccia, L. ; and Cioli, D. "Synthesis and Biological Properties of Some 6H—Pyrido [4,3-b] carbazoles," J. Med. Chem. 1987, 1204-1210. A great deal of interest has been shown in the alkaloids ellipticine (1; 5,ll-dimethyl-6-H- pyrido [4,3-b] carbazole) and its regioisomer, olivacine (2; l,5-dimethyl-6H-pyrido [4,3-b] carbazole), because of their antitumor properties in animals and humans. Ellipticine has been shown to react with DNA by an intercalation process which may account for its cytotoxicity. It has also been found that 1 markedly inhibited DNA polymerase but not RNA polymerase. At concentrations of 0.2 and 1.0 micrograms/mL, the drug inhibited DNA and RNA synthesis as measured by the incorporation of [ 3 H]thymidine and [ 3 H]uridine. At these concentrations there was little effect on protein synthesis. It was concluded that inhibition of nucleic acid synthesis was an important contribution to the cytotoxic effect of ellipticine. See: Suffness, M. ; Cardell, G. A. The Alkaloids : Brossi, A., Ed.; Academic: New York, 1985; Vol. XXV; LePecq, J-B.; Dat Xoung, N. ; Gosse, C; Paoletti, C. Proc. Natl. Acad. Sci. U.S.A. 1974, 71, 5078. Maftouh, M. ; Besselievre, Monserrat, B.; Lesca, P.; Meunier, B.; Husson, H.P.; Paoletti, C. J. Med. Chem. 1985, 28, 708; and Li, L. M. ; Cowie, C. H. Biochem. Biophys. Acta 1974, 353, 3751. One author (Sethi, V. S. Biochem. Pharmacol. 1981, 30, 2026) was able to show that 1 did inhibit RNA polymerase but at concentrations far higher than those of other antitumor agents such as dactinomycin, adriamycin, and daunomycin. . R4 OH Compound 2. was found to produce DNA double and single strand breaks in L1210 cells exposed to the drug, and that this compound was a more active antitumor agent than 1. Compounds 2 and 4 were also found to be active antitumor agents in vivo. Compound ϋ was found to be cytotoxic. Compound 1 was found to be inactive in vivo against murine L1210 leukemia. Compound _L was also found to be inactive. In other studies on the mode of antischistosomal and antitumor action of lucanthone (ϋ) and hycanthone C£) and its cogeners, evidence was found that the methyl group of lucanthone (8.) is metabolized in the mammalian host to hycanthone (2.), which then may be enzy ically esterified to either 2SL or H_. These maybe dissociate to nonenzymically to the carbonium ion 12, which alkylates DNA to form the adduct 11- The carbamate ester 14 acted as a surrogate for _. or H. The enhanced antitumor action of _L5_, in which the 7-OH is regiochemically analogous to the 9—OH in ji, was attributed to stronger intercalation into DNA as compared with hycanthone. See: Archer, S.; Yarinsky, A. Prog. Drug. Res. 1972, 16, 12; Cioli, D. ; Pica-Mattoccia, L. ; Rosenbueg, S.; Archer, S. Life Sci. 1985, 37, 161; Archer S.; Zayed, A. H. ; Rej , R. ; Rugino, T. A. J. Med. Chem. 1983, 26, 1240; and U.S. Patent 4,539, 412 to Archer. In view of the foregoing results, the role of the methyl groups in the ellipticine series is believed to be very important. One hypothesis to rationalize the lack of activity of compound is that the lack of a C-5 methyl group does not permit the metabolic conversion to a hydroxymethyl group, which on enzymic esterification would be converted to an alkylating agent similar to compounds ___£) and 11. Summary of the Invention To examine , the hypothesis that metabolic conversion of the 5— ethyl group would lead to covalent binding to DNA, 5—(hydroxymethyl)—11— methyl—6Ξ—pyrido [4,3—b] carbazole (25) and its N-methylcarbamate (27) were synthesized and the effect of these drugs on DNA synthesis in HeLa cells and their antitumor activity in murine P388 lymphocytic leukemia were studied. Experimental results demonstrate that in fact compound 27. does have significant antitumor activity which, in all cases, is better than the activity of ellipticine Q) or 25. The compounds of the present invention are active in several antitumor systems, especially a lung tumor line which is refractory to most antitumor drugs. Compound 2Z in all cases is far more active than ellipticine, to which the inventive compound is related, to adriamycin, a clinically proven antitumor drug, and to compound 2_5_. Furthermore, compound 27. was found to be schistosomicidal in both hycanthone—sensitive and hycanthone-resistant worms whereas compounds 1 and 25. were not. The present invention provides new and useful compounds which possess outstanding antitumor activity in mice and cell cultures and are active against schistosomiasis, these compounds having the structural formula: R' t t ! wherein R is selected from H, lower alkoxy, OH and phenoxy; _.• is selected from-fi and CH. * ; R' • is selected from CH 3 and CR HQOCNHR,» R' • • is selected from H and CR 1 H00CNER 2 ; R 1 , R' 1 , and R' ' ' are different from each other; when R* is CH-, R is other than H; * is selected from H and lower alkyl; and R 2 is selected from lower alkyl, arainoalkyl and phenyl; and their pharmaceutically acceptable salts. As used herein, "lower alkoxy" and "lower alkyl" signify radicals having from 1 to 5 carbon atoms. P_sCris ion of the Preferred Embodiments One preferred embodiment of the present invention comprises derivatives of ellipticine having the structural formula II: CH, I 3 αnet d i d d their pharmaceutically acceptable salts. Another preferred embodiment of the present invention comprises the olivacine derivatives having the structural formula III: CR^OOCNΞRj wherein R is selected from H, lower alkoxy, OH and phenoxy, and R, and - are as defined above, and their pharmaceutically acceptable salts. Other preferred embodiments include a composition for treating schistosomiasis comprising an effecting amount of a compound of the present invention in a pharmaceutically effecting vehicle as well as a composition for treating tumors comprising an effective amount of a compound of the present invention for treating a tumor in a pharmaceutically acceptable vehicle. Yet other embodiments of the invention comprise a method of treating schistosomiasis in a mammal which comprises administering to said mammal a composition of the present invention for treating schistosomiasis, as well as a method for treating a tumor in a mammal which comprises administering to said mammal a composition of the invention for treating tumors. Preferred salts of the present invention are quaternary ammonium salts. In particular preferred embodiments these salts are selected from the alkyl quaternary and dialkylaminoalkyl quaternary halides and acetates. Example 1 Compound 21- as shown in Scheme I, has been found particularly active as an antitumor agent. The preparation of 5—(hydroxymethyl)—11— methyl—6H— yrido[4,3-b]carbazole (25. a precursor of 27) was accomplished by using a modification of the Weller synthesis of ellipticine (Weller, D. D.; Ford, D. W. Tetrahedron Lett. 1984, 23., 2104) as shown in Scheme I. 25 Z : R . CH 8 OH ___: R.CHO •Cϊ: R.CH2 OCNHCH3 30 35 Acid—catalyzed condensation of methyl indole- 2—acetate, with 3—acetylpyridine, furnished the vinylindole _____• This was converted to the quaternary ammonium salts 17—19 with the appropriate halides , Cyclization in methanol containing sodium methoxide, followed by treatment with a quaternary salt of ethyl nicotinate resulted in ring closure and aromatization to afford 20-22. Heating either 20. or 21 with a number of nucleophiles (e.g., thiophenoxide ion) did not furnish the required ester 21 in usable yield. Catalytic hydrogenation of 21 gave 23. in very low yields; the major product contained a reduced pyridine ring with the benzyl group still attached to the nitrogen. Treatment of 22 with nitrosodimethyl— aniline, as described by Krohnke ( rohnke, F. Chem. Ber. 1938, 71, 2583) to give the nitrone 24 and the desired ester 21 in 47% yield, accompanied by a red by—product, which was difficult to remove chromato- graphically. Reduction of the crude ester with LAH gave the required alcohol 25. in. greater than 50% yield for the two steps. Oxidation of 21 with __n0 2 gave the aldehyde ___, a natural product whose synthesis was reported recently by Gribble (Saulnier, M. ; Gribble, G. Tetrahedron Lett. 1983, 24, 3831). Direct comparison of the infrared spectrum of 26 . with the natural alkaloid showed that the spectra were identical. Treatment of 25. with methyl isocyanate afforded the carbamate 27. Example 2 Ellipticine (1), 5-(hydroxymethyl)-ll-methyl- 6H-pyrido [4,3-b]carbazole (25.), and its corresponding N—methylcarbamate (22) were tested for antitumor activity at the Lederle Laboratories. The results are summarized in Table I. -Si- Table I. Activity against P388 Lymphocytic Leukemia of Ellipticine, δ-.Hydroxymethyl. -ll-methyl-β/f- pyrido[ 4,3-_>]carbazole, and Its Carbamate in Mice" compound dose MST 6 % ILS « placebo 10 control ellipticine (1) 40 16.0 60 20 13.0 30 10 13.0 30 5 12.0 20 25 80. 18 80 40. 16.5 65 20. 16.5 65 10 14.5 45 5 13 30 2.5 12.5 25 27 80. 6.0 toxic 40. 10.0 0 20. 15.0 50 10 21.0 110 5 16.0 60 2.5 18.0 80 "The compounds were administered ip on days 1, 5, and 9 in a 9-day protocol at Lederle Laboratories. BDFR-1 mice were inocu¬ lated ip with 10 6 P388 cells at day 0. MST = median survival time. % ILS = percent increase in life span relative to controls. Table II. Effect of Ellipticine, 5-(Hydroxymethyl)-ll-methyl-6i_ ' -pyrido[4,3-6]carbazole, and Its Carbamate on [ H]Thymidine Incorporation by HeLa Cells" % incorporation of [ 3 H]thymidine compared to control concn, in presence 3 h after compound μg/mL of drug washing ellipticine 5 65 78 10 56 47 25 8 25 ____ 5 67 88 25 22 52 22 5 59 18 25 27 3 "The drugs 1, 25, and 27 were added to growing cultures of HeLa cells and 1 h later [ 3 H]thymidine was added. After another 1 h the amount of the labeled base incorporated by the cells was determined. In another experiment HeLa cells were exposed to the drugs for 1 h and then washed thoroughly. Three hours later [ a H]thymidine was added and the above procedure was repeated. SUBSTITUTE SHEET Ellipticine and the carbinol 25. were about equipotent, but the carbamate 21 was more active than either. The latter exhibited significant antitumor activity at a dose of 2.5 mg/kg and was toxic at the 80 mg/kg dose level. The effect of these drugs on -> [ H]thymidine incorporation in HeLa cells is summarized in Table II. Ellipticine and the carbinol 25. blocked [ H]thymidine incorporation, but the inhibition was partially reversible 3 hours after washing. The carbamate 21 blocked L H]thymidine incorporation also, but in this instance the blockade was irreversible. Compound 21 blocked uridine and thymidine uptake in schistosomes and was more active than either ellipticine 1 and compound 25.- This result is similar to that obtained with hycanthone (2.) and its carbamate _L4 in schistosomes and HeLa cells. Hycanthone blocked incorporation of [ H]uridine, but, after washing, incorporation resumed, whereas the carbamate 14 was effective in 3 preventing [ H]uridine incorporation in washed and unwashed cells. On the basis of this evidence, and alternate mechanism is proposed (Scheme II) to account for the antitumor activity of ellipticine and some of its active congeners . Ellipticine is metabolically cpnverted to 9—hydroxyellipticine (3), a known metabolite of 1 , (Rheinhold, V.; Bittman, L. ; Bruni, R. ; Thurn, K. ; Silveria, D. Proc. Am. Assoc. Cancer Res. 1975, 16 135 and Lesca, P.; Lecointe, P.; Paoletti, C; Mansuy, D. C. R. Acad. Sci .. Ser. D 1976, 282, 1457). This species, in turn, is enzymically converted first to the carbinol 28., which then is transformed enzymically to 22.. This compound, which now possesses a good leaving group, alkylates a nucleophilic macromolecule such as DNA or possibly topoisomerase II to give 212. The carbamate 21 acts as a surrogate for 3J) just as hycanthone N-methylcarbamate (14) does for the corresponding phosphate _____ or sulfate 11. Scheme II __f : H: __£ Such a mechanism can account for the greater antitumor potency and toxicity of 21 if it is assumed that the observed irreversible binding in HeLa cells is due to alkylation of a macromolecule. One author (Ros, W. E. Biochem. Pharmacol. 1985, 34, 4191) has pointed out that ellipticine causes a higher frequency of DNA strand breaks than adriamycin, yet it is far less cytotoxic. He suggests that ellipticine induced breaks are rapidly repaired when the drug is removed from the surrounding medium whereas the adriamycin-induced breaks are retained much longer. If the carbamate 21 forms covalent bonds with macromolecules as in 20, it should be retained much longer than ellipticine or the carbinol 2__L, consequently, repair should occur more slowly, if at all and thus is more active than adriamycin. • yam le Additional tests comparing the activity of adriamycin, ellipticine, compounds 25. and 22 have been conducted, and demonstrate the superiority of compound 22- The results of these experiments are shown in Table III. Tests have also been conducted which confirm the activity of compound 22 against schistosomiasis (a type of worm) . Synthetic index as % of control 3H—uridine ^C—uridine H—thymidine Sens. Res . Sens . Res . Sens . Res. Ellipticine 100 100 100 77 25. 100 70 92 100 44 40 27 18 18 56 47 25 27 In vitro survival on the 30th day of culture: Sensitive Resistant Ellipticine all alive all alive 25 all alive all alive 22 4 dead/6 3 dead/6 S. Mansoni adult male worms were exposed for 1 hour in vitro to 50 micrograms/ml of the drug (some drug re— recipitated in the medium), washed and incubated overnight in drug—free medium. At the end of overnight incubation, the radioactive precursor was added for 1 hour and the trichloroacetic acid-soluble and trichloroacetic acid-precipitable radioactivity associated with schistosomes was determined. Synthetic index was calculated according to Pica-Mattoccia &. Cioli (Mol. Biochem. Parasitol. 8.:99, 1983). Values represent the synthetic index expressed as a 7. of the control untreated schistosomes . Table III Percent Survival of Various Lung Cancer Cell Lines After One Hour Exposure to Drugs Followed by Washers Cell Line* Cone. Compound (μmol > ) H-69 N-417 H-460 H Adriamycin 0.22 95.7 86.5 51.0 6 2.2 50.5 80.3 27.0 4 22 14.5 39.0 0.8 Ellipticine (1) 0.0033 100 94.6 73.1 5 0.033 43 1.9 0.8 1 0.33 9.7 0 0.2 Compound 25 0.0033 92.5 109.3 94.5 9 0.033 39.6 40.7 18.9 5 0.33 16.5 9.1 0.6 1 Compound 27 0.0033 52.9 25.1 10.1 2 0.033 31.6 4.2 0.6 0.33 16.2 3.5 0 Sensitivi .ty of Cell Lines to Drugs Cell Line *Description R H-69 Small cell (Generally refractory) N-417 Variant small cell (Generally sensitive) H-460 Non—small cell (Generally responsive) H-358 Non-small cell (Refractory), Example 4 Synthesis of 5-hydroxymethyl-ll-methyl—6H—pyrido [4,3-b] carbazole N-methylcarbamate (27) l-r2-(Carbometho__ymethyl')-3-indolyl1-l- (3-ρyridyl ' ) ethene (16): A solution of 6.13 g (0.032 m) of methyl indole-2 acetate, 7.16 g (7.0 mL, 0.059m) of dry MeOH was refluxed for 2 hours in an atmosphere of N 2 - The clear red solution was poured onto 600 g of ice. It was made alkaline with NH,0H and extracted with 2 X 500 mL portions of ether. The ether solution was washed with H-,0, dried, and concentrated to dryness. The residue was triturated with hexane-ether (1:1), and the crystals that formed were collected and dried; wt 8.0 g; mp 154-157°C (lit. mp 160-161 β C). The material was suitable for the next step. An additional 110 mg separated from the ether-hexane filtrate. Total yield 8.17 g. 2—(p—Nitrobenzyl)—5—carbomethoxy-11-methyl- 6H-pyrido [4,3—b] carbazolium Bromide (22): Three grams (0.01 mol) of the ester 16. and 12.0 g (0.05 mol) of p—nitrobenzyl bromide in 140 mL of reagent grade acetone was stirred for 24 h. The crystals of 19 were collected, washed with ether, and dried; wt 4.72 g (92%). To a solution of 150 mg of metallic sodium in 40 mL of dry MeOH there were added 2.67 g(5.25 mmol) of crude 2_ and 4,52 g (18.4 mmol) of ethyl nicotinate methobromide. The solution was stirred for 22 hours at room temperature in a nitrogen atmosphere. The crystallize solid that separated was filtered, washed with methanol, and dried; wt 2.31 g (80%) of the desired p—nitrobenzyl quaternary salt 22- The analytical sample was obtained by crystallization from CH 2 C1 2 — eOH (1:1): mp 284-289 β C dec; NMR (Me 2 S0-d 6 ) δ 12.13 (s, 1 H), 10.40 (s, 1 H), 9.31 ' (d, 1 H), 8.69 (d, 1 H), 8.49 (d, 1 H), 8.33 (d, 2 H), 7.86 ( , 3 H), 7.7-7.65 (m, 1 H), 7.50-7.47 (m, 1 H), 6.20 (s, 2 H), 4.13 (S, 3 H), 3,41 (s, 3 H); IR (KBr) 3240, 3045, 2945, 1717, 1590, 1420 cm ~ 1 . 5-Carbomethoxy-ll-methyl-6H-pyrido T .3-bl carbazole (23): To a solution of 200 mg (13 mmol) of metallic sodium in 600 mL of dry MeOH there were added 2.70 g (5.3 mmol) of the quaternary salt 22, 1.06 g (6.3 mmol) of p—nitrosodi ethylaniline, and 300 mL of dry CHCl . The suspension was stirred overnight at room temperature in a nitrogen atmosphere and then evaporated to dryness. The residue was suspended in a solution of 30 mL of CHCl and 5 mL of MeOH and flash chromatographed on a column of 40 g of silica gel with ether-triethyl- amine (20:1) as the eluant. The purest fraction was set aside and the less pure material was rechromatographed. The aside and the less pure material was rechromatographed. The process was repeated, and the purest fractions which showed essentially only one spot on TLC were combined; wt 715 mg (47%). After crystallization from Et0Ac-CH 2 Cl 2 (5:1), the yellow needles melted at 203-204°C dec: NMR (Me 2 S0-d 6 ) 11.56 (s, 1 H) , 9.73 (s, 1 H), 8.87 (d, 1 H) , 8.53 (d, 1 H), 8.38 (d, 1 H), 7.80 (d, 1 H), 7.60-7.52 (m, 1 H) , 7.30-7.29 ( m , 1 H), 4.10 (s, 3 H), 3.33 (s, 3 H) ; IR (KBr) 3300, 2950, 1675, 1600, 1465 cm -1 . 5—(HydroxymethyD—ll-methyl-6H—pyrido r 4.3-b1 carbazole (25~): A solution of 170 mg (0.74 mmol) of metallic sodium in 150 mL of MeOH, 1.78 g (3.5 mmol) of the quaternary salt 22, 581 mg (3.5 mmol) of p—nitrosodimethylaniline, and 75 mL of dry CHCl was stirred for 5 hours at room temperature in an atmosphere of N 2 . The suspension was evaporated to dryness and the dried residue was dissolved in 100 mL of dry THF. To the resulting solution there was added 300 mg of LAH. After the mixture was stirred for 1 hour at room temperature, 600 mg of LAH was added. After 20 min the reaction was judged to be complete (TLC). The mixture was worked up in the usual way, and the solid that was collected was washed thoroughly with five portions of hot CH 2 Cl 2 -Me0H (1:1). The aqueous filtrate was extracted with 3 X 200 mL portions of Ch 2 Cl 2> The combined organic layers were concentrated to dryness, and the residue was suspended in 30 mL of CH 2 C1 2 and 5 Ml of MeOH. The suspension was flash chromatographed on silica gel, first with EtOAc as the eluant and then with EtOAc-MeOH (5:1). The purest fractions were combined, concentrated to a small volume, and cooled, whereupon the desired carbinol crystallized to give 518 mg (56% for the two steps) of 21, which melted at 257-258 β C dec after one crystallization from EtOAc-MeOH (5:1): NMR (Me 2 S0-d 6 ) 6 11.46 (s, 1 H), 9.71 (s, 1 H), 8.43 (d, 1 H), 8.39 (d 1 H), 8.08 (d, 1 H), 7.62-7.48 (m, 2 H), 7.30-7.22 (m, 1 H), 5.25 (s, 3 H) , 3:32 (s, 3 Ξ). 5-Formyl-11-meth l-6H-p rido T4.3-b1 carbazole (17-0xoellipticine) (26): A suspension of 40 mg of the carbinol 21 and 200 mg of MnO-, in 35 mL of CHC1-, was heated under reflux for 3.5 h. The hot suspension was filtered and the collected solid was washed with CHClg. The combined filtrates were evaporated to dryness to leave a residue, which was chromatographed on silica gel. Elution with EtOAc furnished 23 mg of the desired aldehyde 22, which melted at 274-276°C (lit mp 275-276°C) after crystallization from CΞClg—hexane. The IR spectrum was identical in all respects with that of an authentic sample. Further elution of the column with EtOAc-MeOH (19:1) gave 11 mg of recovered starting material. 5-(Hydroxymethyl -ll-methyl-6H-pyrido T4.3—bl carbazole N-Methylcarbamate (27.: To a solution of 400 mg (1.53 mmol of the carbinol 25 in 25 mL of dry pyridine and 20 mL of reagent grade acetone there was added 900 uL of MeNCO. The solution was magnetically stirred at room temperature in a stoppered flask until all the starting alcohol had disappeared as judged by TLC (ca. 3 days). The solvents were removed in vacuo, and the residue was crystallized from EtOAc-CH 2 Cl 2 -MeOH to give 152 mg of the desired carbamate, mp 213-214.5 β C. The filtrate was concentrated to dryness and the remaining solid was flash chromatographed to give an additional 145 mg of material of similar purity: wt 307 mg (61%); NMR (Me 2 S0- 6 ) 11.62 (s, 1 H) , 9.71 (ε, 1 H), 8.46 (d, 1 H), 8.39 (d, 1 H) , 7.97 (d, 1 H), 7.61-7.54 (M, 2 H), 7.32-7.29 (m, 1 H) , 7.0* (d, 1 H), 5.77 (s, 2 H), 3.34 (s, 3 H) , 2.60 (s, 3 H) . In a similar fashion treatment of 22 with ethyl isocyanate, n—propyl isocyanate, isopropyl isocyanate, butyl isocyanate, t-butyl isocyanate, amyl isocyanate and phenyl isocyanate gave 28 HR where R=H and R, = ethyl, propyl, isopropyl, n-butyl, t-butyl, n-amyl and phenyl, respectively. Example 5 Preparation of 5-hydroxyethyl-ll-methyl-6H- pyrido [4,3—b] carbazole N-methyl carbamate (31). A solution of the aldehyde 21 in dry ethyl ether was stirred and cooled in an ice—bath as a solution of methylmagnesium iodide in dry ethyl ether was added slowly. After all the Gregnard reagent was added, the mixture was stirred at room temperature and then poured carefully onto ice— ater. The ether layer was separated, washed with NaHC0~ solution and evaporated to dryness. The residue was chromatographed on a silica gel column and the desired 5—hydroxyethyl-11-methyl 6—H-pyrido [4,3-b] carbazole was collected and dissolved in a solution of dry py idine and dry acetone. Excess methyl isocyanate was added and the reaction mixture was stirred until the alcohol was completely consumed as judged by thin—layer chromatography. The solvents were removed in vacuo and the described compound was purified by crystallization from ethyl acetate- methylene chloride. Examples 6 and 7 9—Methoxy—5— ydroxymethyl—11—methyl—6H—pyrido [4,3—b] carbazole N—methyl carbamate 22 and the corresponding 9—hydroxy compound 4__. were prepared as shown in Scheme III. Methyl 5-methoxy indole-2-carboxylate (32): six grams of 5-methoxy indole—2-acetonitrile was dissolved in 60 mL of methanol and the solution was cooled in an ice—bath while dry HC1 gas was bubbled into the solution. After 30 minutes the mixture was heated to 40-45 β C while the HC1 was still being added for an additional 3 hours. The flask was stoppered and allowed to stand at room temperature overnight. The next day most of the methanol was removed in vacuo and the residue was dissolved in ether. The ether solution was thoroughly washed with water, NaHCO solution and again with water. The dried ether extract was taken to dryness and the residue was chromatographed on silica gel using ethylacetate/hexane as the developing solvent. There was obtained 6.0 g of the desired methyl ester, m.p. 94—95°C after crystallization from benzenehexane. Scheme 171 1—r2-Carbomethoxymethyl—5-methoxy—3—indolyll— 1—(3-ρyridyl ethene (33): To a solution of the ester 32 (5.5 g) and 9.5 mL of 3—acetylpy idine there was added carefully 12 mL of cone, sulfuric acid and the resulting solution was refluxed gently for 2 hours in an atmosphere of nitrogen and allowed to stand at room temperature for an additional 5 hours . The mixture was poured onto water and then made basic with NH,0H. The suspension was extracted with ether and the ether extract was washed with water, dried and evaporated to dryness to leave a brown oil which was triturated with a solution of ether—hexane, whereupon the oil crystallized. The white solid was filtered, washed with hexane and dried m.p. 154-156°C (wt. 1.5 g). An analytical sample was obtained by chromatography on silica gel (30% ethyl acetate/methylene chloride, followed by crystallization from aqueous methanol. m.p. 164-165 β C. 9— ethoxy—5-carbomethoxy-ll-methyl-6H-pyri o T4.3—bl carbazole (35): Seven grams of the pyridyl ethene _1 was dissolved in 200 mL of dry acetone and 14.0 g of p. nitrobenzyl bromide was added. The solution was stirred under nitrogen for 48 hours. Ether was added to the suspension which was filtered and washed with dry ether to give 11.64 g of the crude salt 31. A solution of 11 g of the above quaternary salt was dissolved in 150 mL of dry methanol and 0.7 g of sodium metal (cut into small pieces to facilitate dissolution) was added. When all the sodium had dissolved, ethyl nicotinate methiodide (prepared from 20 g of ethyl nicotinate and 20 L of methyl iodide in dry acetone) was added and the mixture was stirred at room temperature under nitrogen for 24 hours. At the end of that time the crystals that separated were filtered, washed with fresh methanol and dried to form wt. 9.98 g of the cyclized ether 34. A suspension of 3.0 g of the quaternary salt 34 in 500 mL of dry methanol was stirred while 400 m of small pieces of sodium metal was added. This was followed by a solution of 1.25 g of p-nitrosodimethyl- aniline in 300 mL of dry chloroform. The reaction mixture was stirred under nitrogen for 24 hours. The solvents were removed under reduced pressure at 30-35°C and the residue was partitioned between 200 mL of methylene chloride and water, the organic layer was washed with water, dried and evaporated to dryness. The ester was obtained after careful chromatography to remove red by-products . the analytical sample was obtained by crystallization from ethyl acetate. The yellow crystals of 25. melted at 178-179°C. 9—Methoxy—5—hydroxymethyl—11—methyl—6H—pyrido T4.3-b1 carbazole (36): To a solution of 600 mg of the ester H in freshly distilled dry tetrahydrofuran there was added portion wise, 200 mg of lithium aluminum hydride. The reaction mixture was heated under reflux for 2 hours and stirring was continued at room temperature for an additional 2 hours. And additional quantity of 100 mg of lithium aluminum hydride was added. An hour and 30 minutes of stirring at room temperature no more starting ester was detectable by thin layer chromatography. Ethyl acetate was added to destroy the excess lithium aluminum hydride. Water (5 mL) was added followed by 5 mL of 2N sodium hydroxide. After stirring at room temperature for 30 minutes the mixture was filtered and the solid was washed with tetrahydrofuran followed by methanol-chloroform (1:9). The filtrates were combined and dried over sodium sulfate and taken to dryness. After chromatography of the residue using ethyl acetate followed by methanol/ethyl acetate (1:9) there was obtained 297 mg of the desired alcohol 2__ which after crystallization from ethyl acetate melted at 208-209 β C. 9—Methoxy—5—hydroxymethy1—11—methy1—6H-pyrido [4.3-bl carbazole-N-methyl carbamate (37): The above alcohol 3__i was dissolved in dry pyridine and dry acetone and treated with methyl isocyanate as in the case of the carbinol 25.- The mixture was stirred until no further alcohol 2ϋ could be detected on thin layer chromatography. The solvents were evaporated and the residual solid was crystallized from ethyl acetate-methanol to give the desired carbamate 37. 9-Methoxy-ll-methyl-6H-pyrido T4.3-b1 carbazole—5—carboxaldehyde (38): A solution of 250 mg of the hydroxymethyl compound _____ was refluxed in 150 mL of acetone until all was in solution. One gram of manganese dioxide was added. The mixture was refluxed for about 4 hours and was then filtered while still hot. The insoluble material was washed with acetone. The combined acetone filtrates were evaporated to dryness to leave a crystalline residue which after recrystallization from methanol melted at 252-254 β C. 9-H droxy-l1-methyl-6H-pyrido r4.3-b1 carbazole-5-carboxaldehvde (39): a solution of 1.6 g (0.6 mL) of boron bromide in 5 mL of dry methylene chloride was added dropwise to a stirred suspension of 200 mg of the methoxyaldehyde 3_8 in 50 mL of dry CH 2 C1 2 at —78°C. The stirred suspension was allowed to warm to room temperature and stirred for an additional 6 hours. The mixture was cooled in an ice bath and about 5 mL of ice-water was added after 30 minutes the methylene chloride layer was separated under nitrogen. The aqueous brown suspension was cooled in an ice-bath and made alkaline with 15 mL of 10% sodium hydroxide under nitrogen. The dark red solution was taken to pH 7—8 with the aid of acetic acid, sodium bicarbonate solution. The brown precipitate was collected on a filter, washed with water and dried. The dry solid was triturated with methylene chloride-hexane (1:1) and filtered to give the desired crude phenolic aldehyde. Wt. 170 mg, m.p. 346-349 β C. 9-t-Butyldiphenylsilyloxy-ll-methyl-6H- pyrido r 4.3-b1 carbazole-5-carboxaldehyde (40): A solution of 106 mg of the phenolic aldehyde 29 and 680 mg of imidazole in 10 mL of dry dimethyl formamide was stirred at 80 β C in a nitrogen atmosphere while 212 microliters of t—butyl diphenylchlorosilane was added. The mixture was stirred at 80 β C for 40 hours, before being cooled and treated with 100 mL of ether. The organic layer was washed with water and the aqueous extract was back extracted with ether. The combined ether extracts were washed with water and then evaporated to dryness to leave a solid which was triturated with hexane to leave 150 mg of the desired product. -Evaporation of the hexane filtrate left a solid which after thin layer chromatography gave an additional 28 mg of the desired aldehyde. Total yield 178 mg. After crystallization from ethyl acetate there was obtained 103 mg of pure __jp_ m.p. 241-243°C. 9—t-Butyldiphenylsilyloxy—5-hydroxymethyl-ll- methyl-6H-pyrido r 4.3-b1 carbazole (41): A solution of 115 mg of the aldehyde 3_8 in 50 mL of ethanol was stirred at room temperature while 50 mg of sodium borohydride was added in one portion. After one hour at room temperature the reaction mixture was poured into water and 5 mL of 5% ammonium chloride solution, stirred for 10 minutes and extracted with methylene chloride. The organic extract was washed with water and taken to dryness to give 116 mg of crude carbinol. Recrystallization from ethanol gave 42 mg of pure 41 m.p. 208-210 β C. An additional 34 mg was obtained from the ethanol mother liquor. 9—H droxy—5—h droxymethy1—11—6H—p rido T4.3-b1 carbazole-N-methyl carbamate (43): A solution of 18 mg of the carbinol 41 in 20 L of dry methylene chloride was stirred while 48 mg of methyl isocyanate and 7 mg of 4—dimethylamino pyridine were added. The reaction mixture was left overnight. The reaction mixture was evaporated to dryness in vacuo and the residue was chromatographed on a preparative thin layer silica gel plate using ethyl acetate-methanol (19/1) as the developing solvent. There was obtained 8 mg of the desired N-methylcarbamate 42 identified by infra-red NMR spectrum. Example 8 Further tests were performed comparing the activity of adriamycin, ellipticine and several 6H— pyrido [4,3-b] carbazoles against various lung cancer cell lines. The results of these experiments are shown in Table IV. TABLE IV PERCENT SURVIVAL OF VARIOUS LUNG CANCER CELL LINES AFTER ONE HOUR EXPOSURE TO DRUGS FOLLOWED BY WASHING Compound Dose, μg/mL fuM/mL) H-69 N-417 H-460 H-35 .6 .4 ELLIPTICINE .2 .7 .0 Compound ___ 5.0 (0.016) 21.5 15.2 9.6 1 1 .7 0.5 (0.0016) 35.9 25.6 19.0 21 .9 Compound 27 0.05 (0.00016) 76.6 57.7 36.9 47.9 143 (0.51) 77.9 19.8 20 24.1 14.3 (0.051) 3 1 .1 15.7 32.8 30.9 1.4 (0.0051) 85.0 67.5 97.4 76.2 12.0 (0.02) 35.2 0.8 0.8 0.9 Adriamvcin 1.2 (0.002) 47.7 29.92 27.0 41 .5 .12 (0.0002) 35.2 45.2 5 1 .0 66.5 SUBSTITUTE SHEET Compound Dose, μg/mL fuM/mU H-69 N-417 H-460 fT-I 8.0 (0.017) 27.9 15.6 21.1 0. 0.8 (0.0017) 35.7 16.2 35.6 50. 0.08 (0.00017) 86.2 72.3 93.9 80. 8.0 (0.018) 23.2 14.4 19.4 32 0.8 (0.0018) 46.6 37.1 42.9 56 0.08 (0.00018) 98.3 95.9 96.0 92 5.0 (0.016) 21.5 15.2 9.6 11 0.5 (0.0016) 35.9 25.6 19.0 21 HCH 3 Compound 21_ - 0.05 (0.00016) 76.6 57.7 36.9 47 5.0 (0.01) 29.7 21.6 21.7 22 0.5 (.001) 49.2 34.4 42.6 38 0.05 (.0001) 86.6 69.6 91.1 9 5.40 (0.015) 3.1 2.7 0.3 0.54 (.0015) 29.8 4.2 1.8 HCH 3 0.05 (.00015) 33.6 23.9 12.2 2 Compound 37 SUBSTITUTE SHEET Compound Dose. μg/mL fuM/mLl H-69 N-417 H---60 H-3 5.40 (.0151 3.1 2.7 0.3 0. .54 (.0015) 29.8 4.2 1.8 0. HCH 3 .05 (.00015) 33.6 23.9 12.2 22. Compound 37 3.7 (.011) 9.9 4.2 0.1 1. .37 (.0011) 20.7 11.6 3.7 8. .037 (0.00011) 37.6 27.1 18.6 29. 5.0 (0.016) 21.5 1 155..22 99..66 1111.. 0.5 (0.0016) . 35.9 2 255..66 1199..00 2211.. HCH 3 Compound 27 0.05 (0.00016) 76.6 5 577..77 3366..99 4477.. 12.0 (0.02) ADRIAMYCIN 36.3 1.2 0.6 1. 1.2 (0.002) 51.8 31.6 27.3 38. 0.12 (0.0002) 62.2 49.1 51.0 62. SUBSTITUTE IEET Example 9 5—Hydroxymethyl—9-methoxy—11-methyl—6H—pyrido [4,3—b] carbazole N-methylcarbonate (37) was prepared by an alternate route as shown in Scheme IV. N " .N-Dimethyl-5-Methoxyindole-2-carboxamide (45) : Twenty mL of S0C1 2 was added to a stirred suspension of 20g of 5-methoxyindole—2-carboxylic acid in 200 mL of dry benzene and the mixture was heated at 50-60°C for 3 h and then allowed to stir at room temperature for 6 h before the solution was evaporated to dryness in vacuo. Dry benzene (100 mL) was added and the suspension was taken to dryness again to remove the last of the S0C1 2 - The residue was dissolved in about 300 mL of dry ether. Gaseous dimethylamine was passed into 60 mL of dry benzene until the weight was increased by 15.Og. The ethereal solution of the acid chloride was added dropwise to the stirred solution of dimethylamine over a period of twenty minutes. The suspension was stirred for 2 h and then treated with water. The layers were separated and evaporation of the ether left a solid which was dried at 70 β C to give 22g of the desired amide, mp 204-206°C (lit. value 207°, J. Med. Chem. (1980) 22, 1330). 2—(N.N- imethylaminomethyl)—5-methoxyindole (46) and Methiodide (47). A suspension of 6.0 g of LiAlH, in dry tetrahydrofuran (200mL) was cooled in an ice—water bath as 16.0 g of the above amide was added portion-wise over a 30 min. period. The mixture was stirred overnight at room temperature before being treated with 10 mL of ethyl acetate. Water (20 mL) was added and after stirring for 20 min the suspension was filtered and the solid washed with 2 X 100 mL portions of ethyl acetate. The combined filtrates were taken to dryness and the residue was dissolved in ether, washed with 20mL of 2N NaOH, H 2 0 and dried over Na 2 S0, . The ether was distilled off to leave 14.4 g of an oil which was dissolved in 100 mL of dry ethylacetate. To this solution there was added dropwise with stirring 5.5 mL of methyl iodide in 40 mL of ethyl acetate. After stirring overnight the mixture was treated with 100 mL of dry ether and filtered to leave 22.5 g of the methiodide, mp. 182-184 β C (lit. value, mp. 184°, J. Med. Chem. (1980) 22, 1330). 5-Methoxy-indole-2-acetonitrile (48) . To a solution of 9.0 g of KCN in 250 mL of methanol there was added with stirring in an atmosphere of nitrogen a solution of 15.0 g of the quaternary salt in 250 mL of methanol. After refluxing for 20 h the solution was concentrated to about 50 mL. Then 300 mL of ether was added and the dark solution was stirred with charcoal, filtered and the filtrate was evaporated to dryness to leave a residue which was chromatographed on silica gel using hexane-ethylacetate (40:60) as the eluant. There was obtained 5.48 g of the nitrile, mp. 85-86°C. Methyl—5—methoxyindole—2—acetate (49) . A stirred solution of 6.0 g of the above nitrile in 60 mL of methanol was cooled in an ice-bath and dry HC1 gas was passed into the solution slowly. The solution was allowed to warm and then was stirred at 40-45 β C for 3 h. The HC1 gas was turned off and the mixture allowed to stand overnight. The methanol was distilled almost to dryness and the residue was dissolved in ether and washed water. The ether layer was dried and taken to dryness to leave a residue which was chromatographed on silica gel using ethyl acetate/hexane (60:40) as the eluant to give 6.05 g of the desired ester, mp. 94-95°C, after crystallization from benzene/hexane. Scheme IV Alternative Synthesis of Compound 37 44 45 46 47 48 49 '" 34 35 36 37 *-_OC^.(CH3)?NH;b: UAI-U : c:CH_I. _ KCN itCHsOH .Hα:1:3-_cerj yr_ ( .ne ,H_SO_ ; g : p - NO_C_H_ CH 2 Br ; h: NaOCH.. Elhyi n otinae ret o&romcJe ; i : (n-tutyl )-?. J: UAIH* : k:CH 3 NCO,DMAP Anal, Calcd. for C 12 H 13 N0 3 (219.2); C, 65.74; H, 5.98; N, 6.39. Found: C, 65.81; H, 6.02; N, 6.39. Methyl-5-methoxy-3- ( l-r3—pyridyll-vinyl)indole-2- acetate (33). A mixture of 5.6 g of the above ester, 9.5 L of 3—acetylpyridine in 200 mL of methanol was treated with 12.0 mL of concentrated H 2 S0, and the solution was refluxed for 7 h. The whole was poured into H 2 0 and make alkaline with NH,0H. The suspension was extracted with ether. The organic layer was washed with H-,0, dried and evaporated to leave an oil, which after trituration with ether-hexane, (3:7), solidified. The solid was filtered, washed with hexane and dried to leave 7.5 g of the vinyl indole, mp. 154-156°. After crystallization from 70% aqueous methanol the pure product melted at 164—165 β C. Anal. Calcd. for C 19 H 18 N 2 0 3 (322.4); C, 70.79; H, 5.63; N, 8.69. Found: C, 70.67; H, 5.69; N, 8.65. Adduct of Methyl-5-methoxy-3- ( l-r3-pyridyl ~ l-vinyl)- indole—2-acetate and p—Nitrobenzyl Bromide (50). A mixture of 7.0 g of the vinyl indole and 14.0 g of p-Nitrobenzyl bromide in 200 mL of acetone was stirred under nitrogen for 2 days, then filtered. Dry ether (100 mL) was added and the yellow precipitate was collected and dried. Wt 11.64 g, mp ca 185°C dec. 5-Carbornethoxy-9-methoxy-l1-methyl-6H-pyridof .3-b1 carbazole—2-p-Nitrobenzyl bromide (34). A solution of 1.47 g (2.73 mmol) of the vinyl indole methobromide and 2.72 g (11.1 mmol) ethyl nicotinate methobromide was stirred under argon as 42 mL of 0.3M (12.6 mmol) sodium methoxide was added in one portion. The mixture was stirred at room temperature for 22 h and then filtered. The solid was washed with 4 X 10 L portions of methanol 2 X 10 mL portions of H 2 0 and again with 4 X 10 mL portions of methanol and dried, wt. 1.37 g. The combined filtrate and washings were diluted with ether and allowed to stand whereby a second crop separated, wt. 0.067 g. The total yield of material pure enough to be used in the next step was 1.43 g (97%), mp. 260-280°C dec. 5-Carbomethoxy—9-methoxy—ll-methyl-6H-pyridoT4.3-bl carbazole (35). A. To a suspension of 3.0 g of the quaternary salt 4 in 500 mL of methanol in which there was dissolved 1.26 g of p—nitrosodimethylaniline there was added 400 mg of sodium in small pieces. After all the sodium had dissolved 300 mL of dry chloroform was added and the mixture was stirred under nitrogen for 24 h. The solvents were removed under reduced pressure and the residue was partitioned between methylene chloride and water. The organic layer was evaporated to dryness and the residue was flash chromatographed on a silica gel column. The nitrone and other by—products were removed with ethyl acetate- chloroform (20:80) and the desired ester was eluted with ethyl acetate. Careful rechromatography gave 1.35 g of the ester, which melted at 163-165"C. After crystallization from ethyl acetate it melted at 178-179 β C. Anal. Calcd. for c χ9 H i N 2 0 3 (320.3): C, 71.23; H, 5.04; N, 8.75. Found: C, 71.11; H, 5.12; N, 8.68. B. A mixture of 3.69 g of the above ester, 4.22 g (20.9 mmol) of tributylphosphine and 52 mL of dimethylformamide was refluxed for 15 min. in n inert atmosphere and then cooled. The solvents and excess tributylphosphine were removed at room temperature (0.1 mm) and the residue was partitioned between CH 2 C1 2 and H 2 0. The CH 2 C1 2 layer was evaporated to leave a black residue which was boiled first with benzenehexane (1:1). The solvent was decanted and the residue was extracted with boiling benzene, the extracts were evaporated and the residue finally was extracted with hexane. The hexane extracts which contained the remainder of the tributylphosphine and tributylphosphine oxide were discarded. The residue was chromatographed in a silica gel column using CH 2 C1 2 as the eluant. The major fraction which appeared as a yellow band weighed 1.29 g (58%), mp. 165°C. The IR spectra of the two samples were identical. 5-Hydroxymethyl—9-methoxy—ll-methyl-6H-pyrido .4.3-blcarbazole (36). A solution of 600 mg of the above ester in 100 mL of dry tetrahydrofuran was stirred under N 2 as 200 mg of LiAlH, was added portion—wise with stirring. The mixture was stirred for 30 minutes and then cooled in an ice-bath while 3 mL of ethyl acetate was added dropwise followed by 5 mL of H 2 0 and then 5 mL of 2N NaOH. The mixture was stirred for 30 min. , filtered and the solid washed 2 times with 30 mL of tetrahydrofuran and then with methanol-chloroform (1:9). The combined filtrates were evaporated and the residue was chromatographed on a column of silica gel using a gradient of methanol-ethyl acetate (5-10% methanol) to give 297 mg (54%) of the desired alcohol. An analytical sample was recrystallized from ethyl acetate, mp. 208-209 β C (dec). Anal. Calcd. for c χ8 H 10 N 2°2 (292.3): C, 73.95; H, 5.52; N, 9.59. Found: C, 73.83; H, 5.57; N, 9.56. 5—Hydroxymethyl—9—methoxy-11—methyl—6H—pyrido F4.3-b " Icarbazole N-methylcarbonate (37). A solution of 29.8 mg of the above alcohol and 14.4 mg of 4—dimethylaminopyridine in 60 mL of dry methylene chloride was stirred at room temperature for 1 h before 135 μL of re-distilled methyl isocyanate was added. The mixture was stirred for 18 h, filtered and the filtrate was concentrated to about 10 mL and the solid which separated was collected, washed with cold methylene chloride and dried. Wt.=20.0 mg (56%, mp. 196-197°C (dec.)). Anal. Calcd. for C 20 H 19 N 3°3 H 2 0 (367.39): C, 65.38; H, 5.76; N, 11.44. Found: C, 65.52; H, 5.48; N, 11.34. Example 10 Preparation of 1—Hydroxymethyl-9-methoxy—5-methyl- 6Ξ-pyrido [4,3-b] carbazole N-methylcarbamate (53) H CH, 52 a:LiAlH 4 ; b: CH 3 NC0,DMAP Scheme V l-Hydroxymethyl-9-methoxy-5-methyl—6Ξ-pyridor4.3-bl carbazole (52). To a cooled suspension of 180 mg (4.74 mM) of LiAlH, in 120 mL of dry THF there was added 255 mg (0.88 mM) of 1-formyl-9-methoxy-5—methyl-6H-pyrido [4,3,-b]carbazole (51) followed by 25 mL of THF. The reaction mixture was allowed to warm to room temperature and stirring was continued for a total of 2 h. The mixture was treated with 85 mL of H 2 0, 85 mL of 10% NaOH followed by another 250 mL of H 2 0. The THF was removed in vacuo and the solid was collected and extracted continuously in a Soxhlet apparatus with 5% MeOH in benzene for 10 h. The extract was evaporated and the residue was chromatographed on silica gel using ethyl acetate-hexane as the eluant to furnish 150 mg (58%) of the alcohol 52, mp. >300 β C. IR (KBr): 3400-3080, 2920, 1620, 1480, 1395, 1283, 1200, 1030, 810, 765 cm -1 . NMR (DMS0-d & ) δ 11.18 (1H, s, NH); 8.97 (1H, s, __ n >; 8.32 (1H, d, J=6.2. H 3 ); 7.92-7.86 (2H, m, Ξ 4 H 1Q ); 7.44 (1H, d, J=8,6, H 7 ), 7.13 (1H, dd, J=2.5, 1.7, H g ); 5.35 (1H, t, OH), 5.20 (1H, d, J=5.2, CH 2 0H); 3.88 (3H, s, 0CH 3 ), 2.80 (3H, s CH 3 ); 3.31 (s, H 2 0). MS m/e 293 (M+l). 1—Hydroxymethyl—9—methoxy—5—methyl—6H— yridoT .3—bl carbazole-N-methylcarbamate (53). To a suspension of 30 mg (0.10 mM) of the alcohol _. and 15 mg (0.12 mM) of 4-dimethylaminopyridine in 60 mL of dry CΞ 2 C1 2 there was added 1.5 mL (0.025 M) of freshly distilled methyl isocyanate. The mixture was stirred overnight at room temperature and the solvent removed in vacuo. The same solid residue was chromatographed on silica gel using ethyl acetate-hexane as the eluant. There was obtained 14 mg (39%) of the desired product 52, mp. 220-224 β C; IR (KBr) 3295, 2950, 1680, 1475, 1260, 1200, 1090, 1030, 790 cm -1 ; NMR (CDC1 3 ) δ 8.40-8.36 (3H, m); 7.69-7.67 (1H, m) ; 7.53 (1H, m) ; 7.39-7.27 (2H, m); 5.87 (2H, s, CH 2 ); 3.97 (3H, s, 0CΞ 3 ); 2.89 (3H, d, J=3.6, NH-CH^) ; 2.58 (3H, s, CH 3 ). Example 11 Preparation of 5—hydroxymethyl—ll-methyl-6H-pyrido [4,3—b] carbazole N—methylcarbamate, diethylamino— ethyl quaternary chloride A mixture of 5—hydroxymethyl-ll-methyl-6H— pyrido[4,3—b]carbazole N—methylcarbamate (15.1 mg, 0.05 mmol) in 1.75 mL of diethanolamine HC1 stock solution in EtOH (0.05 xs) and Hunigs base (35 μl, 0.20 mmol) was stirred at room temperature. When gentle heating did not result in complete solution, 2 mL additional EtOH were added and gently heated with a heat gun. Most of the material went into solution. Reverse phase thin layer chromatography showed mostly product and just a trace of starting material. The product was then concentrated to dryness in vacuo and placed under high vacuum overnight. Reverse phase, high pressure liquid chromatograph gave a bright yellow solid. Example 12 Preparation of 5-hydroxymethyl—ll-methyl-6H—pyrido [4,3—b] carbazole N-methylcarbamate, methyl quaternary iodide A mixture of 5—hydroxymethyl—11—methyl—6H— pyrido[4,3—b]carbazole N—methylcarbamate (10.0 mg, 0.03 mmol) was dissolved in 1 mL dimethylformamide and 1 mL methanol. Methyl iodide (200 mL) was added, and stirring at room temperature was continued over the weekend. Then the methanol was blown off with a stream of nitrogen and the dimethylformamide removed under high vacuum to yield a yellow solid.

Claims: /11480 -37- Cla.as; 1. A compound selected from the 6H-pyrido [4,3-b] carbazoles having the structural formula: wherein R is selected from E, lower alkoxy, OH and phenoxy; R 1 is selected from H and CH 3 ; i 8 selec e from CH 3 and CR 1 H00CNHR 2 ; R' 11 is selected from H and CR^OOCNH g; R' , R' ' , and R' ' ' are different from each other; when R' is CH 3 , R is other than H; R 1 is selected from Ξ and lower alkyl; and R 2 is selected from lower alkyl, aminoalkyl and phenyl; and their pharmaceutically acceptable salts. 2. A compound of claim 1 which is a quaternary ammonium salt, 3. A compound of claim 1 wherein R is CH 3 0, R' is CH 3 , R" is CR j HOOCNHR-,, R' " is H, χ is H and 2 is CH 3 . 4. A compound of claim 1 wherein R is CH 3 0, R' is H, R" is CH 3 , R" * is CR j HOOCNHR-,, 1 is H and ∑ is CH 3 , 5. A composition for treating schistosomiasiβ which comprises a clinically effective amount of a compound of any one of claims 1-4 and a pharmaceutically acceptable carrier. 6. A composition for treating a tumor : which comprises a clinically effective amount of a compound of any one of claims 1-4 and a pharmaceutically acceptable carrier. 7. A method for treating schistosomiasis in a mammal which comprises administering to said mammal a composition of claim 5 in an amount and regimen effective to treat said schistosomiasis. 8. A method of treating a tumor in a mammal which comprises administering to said mammal a composition of claim 6 in an amount and regimen effective to treat said tumor.

Inventors: ARCHER SYDNEY, ARCHER, SYDNEY
Applicants: RENSSELAER POLYTECH INST, RENSSELAER POLYTECHNIC INSTITUTE
Application year: 1989
Main classification: C07D471
IPC codes: A61K31, A61P33, A61P35, C07D471