Antagonists of leukotriene D4

Abstract: This invention encompasses compounds of Formula I and the pharmaceutically acceptable salts and geometrical and optical isomers thereof wherein: Alk is alkylene or hydroxyalkylene containing 1-6 carbon atoms; Ar is 5,6,7,8-tetrahydro-1-naphthalenyl or phenyl optionally substituted with one or more substituents selected from lower alkyl containing 1 to 6 carbon atoms, hydroxy, lower alkoxy containing 1 to 6 carbon atoms, or lower alkanoyl containing 1 to 6 carbon atoms; R is hydrogen and lower alkyl containing 1 to 6 carbon atoms; X, Y, and Z are each independently O or S with S optionally oxidized to S=O; m is an integer from 0 to 3; n is an integer from 0 to 5. The compounds are useful as anti-allergy agents and anti-inflammatory agents.

Description: This invention is in the field of pharmaceutical agents which act as leukotriene D₄ (LTD₄) antagonists and includes embodiments which act as leukotriene B₄ (LTB₄) antagonists. The prior art generally describes LTD₄ antagonists as anti-allergy compounds and LTB₄ antagonists as anti-inflammatory agents. Leukotriene D₄ and C₄ (LTD₄/LTC₄) and leukotriene B₄ (LTB₄) are products of the arachidonic acid metabolic pathway. LTD₄ and LTC₄ are associated with smooth muscle contraction and contract guinea pig ileum, human and guinea pig bronchi and human pulmonary artery and vein. LTB₄ is associated with neutrophil stimulation and is characterized by chemotaxis, aggregation and degranulation. LTB₄ is believed to be an important mediator of inflammation. High levels of LTB₄ are detected in rheumatoid arthritis, gout, psoriasis, and inflammatory bowel disease. Thus antagonists of LTB₄ are useful in the therapy of such diseases. Dioxolane-2-carboxylic acids of the formula where R₁ and R₂ are the same or different H, halogen, alkyl, or alkoxy; R₃ is H, alkyl, aryl or alkyl optionally substituted by halogen, lower alkyl, or lower alkoxy; X is -CH₂-, -OCH₂- where the O is joined to the phenyl; n is 0-3, are taught as sedatives or choleretic agents in French Patent 1,445,013. This invention encompasses novel compounds of the hereinafter described Formula I, pharmaceutical formulations containing such compounds, and the use of these formulations as anti-allergy agents and anti-inflammatory agents. This invention encompasses compounds of Formula I and the pharmaceutically acceptable salts and geometrical and optical isomers thereof wherein: Alk is alkylene or hydroxyalkylene containing 1-6 carbon atoms; Ar is 5,6,7,8-tetrahydro-1-naphthalenyl, or phenyl optionally substituted with one or more substituents selected from alkyl containing 1 to 6 carbon atoms, hydroxy, alkoxy containing 1 to 6 carbon atoms, and alkanoyl containing 1 to 6 carbon atoms; R is hydrogen or alkyl containing 2 to 6 carbon atoms; X, Y, and Z are each independently O or S with S optionally oxidized to S=O; m is an integer from 0 to 3; n is an integer from 0 to 5. A preferred embodiment of the present invention is a compound of the formula and the pharmaceutically acceptable salts and geometrical and optical isomers thereof wherein: Alk is alkylene or hydroxyalkylene containing 1-6 carbon atoms; R is hydrogen or alkyl containing 1 to 6 carbon atoms; X, Y and Z are each independently O or S with S optionally oxidized to S=O; m is an integer from 0 to 3; n is an integer from 0 to 5. The term "alkyl" means straight or branched chain alkyl having 1 to 6 carbon atoms such as methyl, ethyl, propyl, butyl, pentyl, hexyl and the branched chain isomers thereof. The term "alkoxy" means straight or branched chain alkoxy having 1 to 6 carbon atoms such as methoxy, ethoxy, propoxy, butoxy, pentoxy, hexoxy and the branched chain isomers thereof. The term "alkanoyl" means straight or branched chain alkanoyl having 2 to 6 carbon atoms such as acetyl, propanoyl, butanoyl, pentanoyl, hexanoyl and the branched chain isomers thereof. The term "alkylene" means straight or branched chain alkylenes having 1 to 6 carbon atoms such as methylene, ethylene, trimethylene, methylethylene and tetramethylene. The term "pharmaceutically acceptable salts" means non-toxic salts of the acids of the compounds of this invention where the cation is sodium, potassium, lithium, calcium, magnesium, zinc, ferrous, aluminum, ammonium, tetraalkylammonium, and the like. Compounds of this invention are generally prepared according to the following schemes: Scheme A The compounds of Formula I wherein X is S, Y is O and Z is S and remaining definitions are as defined before are prepared according to Scheme A. An appropriate ketoester which is prepared according to the procedure described in Bartlett and Woods, J. Am. Chem. Soc. , 62 . 2933 (1940) is reacted with 2,3-dimercapto-1-propanol in the presence of an organic solvent such as benzene and a mineral acid such as hydrochloric acid. The reaction results in the products which are represented by Formula 2 and Formula 3. The products which are represented by Formula 2 are further treated with 3-(2-n-propyl-3-hydroxy-4-acetyl phenoxy)-1-bromo propane which is described in U.S. Patent 4,565,882 in the presence of an organic solvent such as methyl ethyl ketone and potassium carbonate to give the compound of Formula 4=the compounds wherein the R of Formula I is alkyl containing 1 to 6 carbon atoms=with the proviso that these compounds could be further treated with lithium hydroxide to give compounds of Formula I wherein R is hydrogen. It is understood that the compounds which are represented by Formula 4 and Formula 5 are racemic mixtures. Scheme B The compounds of Formula I wherein X, Y, Z are all S and the remaining definitions are as defined before are prepared according to Scheme B. The compounds which are represented by Formula 3 are treated with thionyl chloride in an organic solvent such as benzene to give the compounds of Formula 6. These compounds are further treated with a mixture of potassium thioacetate in N,N,-dimethylformamide to give the compounds of Formula 7. Conversion of the thioacetate compounds of Formula 7 to the mercaptan compounds of Formula 8 is accomplished by treating the thioacetate compounds with the appropriate sodium alkoxide. Treatment of the mercaptan compounds of Formula 8 with 3-(2-n-propyl-3-hydroxy-4-acetyl phenoxy)-1-bromo propane and potassium carbonate results in the compounds of Formula 9. Treatment of compounds of Formula 9 with lithium hydroxide gives the compounds of Formula 10. It is understood that the compounds represented by Formula 9 and Formula 10 are mixtures of racemates. Scheme C The compounds of Formula I wherein X is O, Y is O and Z is S and the remaining definitions are as defined before are prepared according to Scheme C. A ketoester which is prepared according to the procedure described in Bartlett and Woods, J. Am. Chem. Soc. , 62 , 2933 (1940) is reacted with glycerol in the presence of an organic solvent such as benzene and p-toluenesulfonic acid to give the compound represented by Formula 11. The compounds represented by Formula 11 are treated with p-toluenesulfonyl chloride in the presence of pyridine to give the compounds represented by Formula 12. The compounds of Formula 12 are reacted with potassium thioacetate in the presence of acetone to give the compounds of Formula 13. Compounds of Formula 13 are treated with the appropriate sodium alkoxide to give the compounds of Formula 14. Compounds of Formula 14 are reacted With 3-(2-n-propyl-3-hydroxy-4-acetylphenoxy)-1-bromopropane which has been prepared according to the procedure described in U. S. Patent 4,565,882 in the presence methyl ethyl ketone to give the compounds of Formula 15. When the compounds of Formula I wherein R is hydrogen are desired, compounds of Formula 15 can be treated with lithium hydroxide to give the compounds represented by Formula 16. It is understood that the compounds which are represented by Formulas 15 and 16 are racemic mixtures. Scheme D The compounds of Formula I wherein X is O, Y is S and Z is S and the remaining definitions are as defined before are prepared according to Scheme D. A ketoester which is prepared according to the procedure described in Bartlett and Woods, J. Am. Chem. Soc. , 62 , 2933 (1940) is reacted with 3-mercapto-1,2 propanediol in the presence of a mineral acid or Lewis acid such as BF₃ etherate in methylene chloride to give the compound represented by Formula 17. The compounds represented by Formula 17 are treated with p-toluenesulfonyl chloride in the presence of pyridine to give the compounds represented by Formula 18. The compounds of Formula 18 are reacted with potassium thioacetate in the presence of acetone to give the compounds of Formula 19. Compounds of Formula 19 are treated with the appropriate sodium alkoxide to give the compounds of Formula 20. Compounds of Formula 20 are reacted with 3-(2-n-propyl-3-hydroxy-4-acetyxlphenoxy)-1-bromopropane which is prepared according to the procedure described in U. S. Patent 4,565,882 in the presence of methyl ethyl ketone to give the compounds of Formula 21. When the compounds of Formula I wherein R is hydrogen are desired compounds of Formula 21 can be treated with lithium hydroxide to give the compounds represented by Formula 22. It is understood that the compounds which are represented by Formulae 21 and 22 are racemic mixtures. In all schemes 3-(2-n-propyl-3-hydroxy-4-acetylphenoxy)-1-bromopropane was exemplified in that phase of the reaction sequence in which the side chain (left hand side portion of the molecule represented by Formula I) introduction was taught. It is understood that replacement of the bromide compound with a 5,6,7,8-tetrahydro-1-naphthalenyloxy or a phenoxy-alkyl bromide compound and following the reaction sequences which are described in Schemes A, B, C and D will result in the compounds of Formula I wherein Ar is 5,6,7,8-tetrahydro-1-naphthalenyl or phenyl and the remaining definitions are as described. Optical isomers are resolved into enantiomers by conventional techniques. The LTD₄ antagonist utility compounds are illustrated by activity in one or more of the following tests. LTD₄ Antagonism in Guinea Pig Ileum Fresh segments of guinea pig ileum were suspended in 2ml. tissue baths containing oxygenated modified Tyrodes solution. After an equilibration period, an agonist dose-response curve was generated by exposing each tissue to 4 different LTD₄ doses and measuring the ensuing contractile heights. The ileum segments were washed and rested between exposures to agonist. Following this, the tissues were incubated with a single concentration of test compound and the agonist dose-response procedure was repeated. The dose ratio is a measure of the antagonist's ability to shift the agonist dose-response curve to the right. It is derived as the concentration of agonist necessary to reach a given response level in the presence (A′) versus the absence (A) of antagonist. For example, if the test concentration of compound had no effect on the agonist-induced response (A′=A) the dose-ratio would approximate 1. Dose-ratios increase if the compound inhibits the agonist-induced response. One dose-ratio value is determined for each strip of ileum used to test antagonist. If the dose-ratios increase as a function of increasing antagonist concentration, these data may be evaluated by Schild analysis to determine whether the inhibition is competitive and if so, what the pA₂ value is for that compound. Schild analysis examines the linearity of the function described by the dose-ratios written as log [(A′/A)-1] versus antagonist concentration. If the linearity is confined and the slope approximates 1, inhibition is considered to be competitive. The pA₂ is the negative log of the antagonist concentration required to produce a dose-ratio of 2. This value is considered to be a measure of the affinity of the competitive antagonist. In Vivo Assay The compounds are tested in vivo as follows. Adult male fasted Hartly guinea pigs weighing 300-350g are pretreated with pyrilamine and indomethacin to block the bronchoconstricture effects of endogenous histamine and the synthesis of thromboxane A₂ respectively. Compounds of the invention are administered IV or IG at appropriate times prior to the IV administration of 2000 units of LTD₄. Intratracheal pressure is monitored prior to and subsequent to LTD₄ in animals anesthetized with pentobarbital and attached to a rodent respirator. Compounds which antagonize the direct component of LTD₄ action on respiratory smooth muscle inhibit intratracheal insufflation pressure increases (P< or = 0.05) caused by LTD₄. FPL 55712 is used as a control. Leukotriene Receptor Binding Assay The in vitro LTD₄ receptor binding assay is carried out as follows: Specific binding of titrated LTD₄ to a fixed number of guinea pig lung receptors, isolated from guinea pig lung membrane, is measured in the presence and absence of test compound. The initial screening dose (1 x 10⁻⁵ M is considered active if the specific binding of the LTD₄ is reduced by 45% or more. Active compounds are tested in a dose-response manner to determine IC₅₀ values. LTB₄ activity of compounds of this invention is indicated by the following tests. Preparation of Human Neutrophils Neutrophils were purified from venous blood of normal human donors using standard techniques of dextran sedimentation, centrifugation on Histopaque sterile solution (Sigma) and hypotonic lysis of erythrocytes (Boyum, A., Isolation of Leukocytes From Human Blood: Further Observations . Scand. J. Lab. Clin. Invest. 21 (Suppl. 97): 31, 1968). The purity of isolated neutrophils was ≧ 95%. LTB₄ Receptor Binding Assay Neutrophils (4 - 6x10⁶) in Iml Hanks' balanced salt solution containing 10 mM HEPES buffer (HBSS), pH 7.4 and 30 mM nordihydroguaiaretic acid were incubated with 0.6 nano M (³H) LTB₄ in the presence or absence of test compounds. The incubation was carried out at 0°C for 45 minutes and terminated by adding 5ml of ice-cold HBSS followed by rapid filtration of incubation mixture under vacuum through GF/C glass fiber filters.The filters were further washed with 10ml HBSS and radioactivity was determined. Specific binding was defined as the difference between total binding and nonspecific binding which was not displaced by 10⁻⁷ M unlabeled LTB₄. All data refer to specific binding. Human Neutrophil Degranulation Assay Neutrophil degranulation was determined by measuring the release of myeloperoxidase activity into the incubation medium. Neutrophils (3 x 10⁶) in 1ml HBSS solution were preincubated with cytochalasin B(5µg) at 37°C for 5 minutes, followed by preincubation with test compounds for 7 minutes. Neutrophils were then incubated for 2 to 20 minutes with either LTB₄ (5 x 10⁻⁸ M) or the chemotactic peptide f-met-leu-phe (5 x 10⁻⁶ M) to induce degranulation. Following incubation, samples were centrifuged and myeloperoxidase was extracted from the cell pellets by sonication in phosphate buffer containing 0.4% Triton X-100. Triton X-100 was also added to the supernatants to a concentration of 0.4%. The supernatants and the pellet - extracts were then assayed spectrophotometrically for myeloperoxidase activity by determining the rate of decomposition of H₂O₂ with o-dianisidine as hydrogen donor as described by Renlund, et al. (Renlund, D.G., Macfarlane, J.L., Christensen, R.D., Lynch, R.E., and Rothstein, G., A Quantitative And Sensitive Method for Measurement of Myeloperoxidase , Clinical Research 28:75A, 1980). Myeloperoxidase activity released into the supernatant was expressed as the percent of the average total activity (pellet plus supernatant). Guinea Pig LTB₄-Induced Dermal Chemotaxis Test compound was administered intravenously or intragastrically at various times prior to the injection of leukotriene B₄ (LTB₄). LTB₄ was diluted in phosphate buffered saline (PBS) and 35ng in 0.2ml were injected intradermally into the shaven backs of anesthetized guinea pigs. PBS was injected as control. Four hours later, animals were sacrificed, skins removed and stored frozen (-70°C). Injection sites were removed with a skin punch and mechanically homogenized (Polytron, Brinkmann Instruments). Myeloperoxidase (MPO), a marker enzyme for neutrophils, was extracted by 0.5% hexadecyltrimethylammonium bromide in 50 mM potassium phosphate buffer (pH 6.0) using sonication and freeze-thaw procedures. After centrifugation (40,000 x g, 30 minutes), enzyme activities in the supernatants were assayed spectrophotometrically (A₄₆₀) by measuring the decomposition of hydrogen peroxide with ortho-dianisidine after 15 minutes. MPO activity was found to be proportional to the number of neutrophils. In guinea pigs the level of MPO activity increased with the amount of LTB₄ injected. Modified Boyden Chamber Chemotaxis Human neutrophils were isolated from citrated peripheral blood sedimented in 5% dextran, followed by centrifugation on Histopaque sterile solution (Sigma) and hypotonic lysis of erythrocytes. A final cell suspension of 3-4 x 10⁶ neutrophils/ml of HEPES-buffered Hanks' balanced salt solution (HBSS, pH 7.3) was added to the upper well (0.8ml) of a modified Boyden chamber (blind well). The lower well (0.2ml), separated by a polycarbonate membrane (Nuleopore Corp.), contained HBSS or 3 x 10⁻⁸M LTB₄ in the presence or absence of test compound. Following a 90 minute incubation at 37°C in 5% CO₂-95% air, cells from the lower well were lysed and nuclei counted in a Model S-Plus-IV Coulter counter. Percent inhibition was calculated from cell counts corrected for random migration by subtracting the mean of the HBSS control. Table I illustrates the LTD₄ and LTB₄ activity of a representative compound of the present invention. The compounds can be administered in such oral dosage forms as tablets, capsules, softgels, pills, powders, granules, elixirs, or syrups. The compounds can also be administered intravascularly, intraperitoneally, subcutaneously, or intramuscularly, using forms known to the pharmaceutical art. In general, the preferred form of administration is oral. For the orally administered pharmaceutical compositions of the present invention the foregoing active ingredients will typically be administered in admixture with suitable pharmaceutical diluents, excipients, or carriers (collectively referred to herein as "carrier" materials) suitably selected with respect to the intended form of administration, that is, oral tablets, capsules, softgels, elixirs, syrups, drops, and the like, and consistent with conventional pharmaceutical practices. For example, for oral administration in the form of tablets or capsules, the active drug components can be combined with any oral non-toxic pharmaceutically acceptable inert carrier such as lactose, starch, sucrose, cellulose, magnesium stearate, dicalcium phosphate, calcium sulfate mannitol, and the like, or various combinations thereof. For oral administration in liquid forms, such as in softgels, elixirs, syrups, drops and the like, the active drug components can be combined with any oral non-toxic pharmaceutically acceptable inert carrier such as water, saline, ethanol, polyethylene glycol, propylene glycol, corn oil, cottonseed oil, peanut oil, sesame oil, benzyl alcohol, various buffers, and the like, or various combinations thereof. Moreover, when desired or necessary suitable binders, lubricants, disintegrating agents, and coloring agents can also be incorporated in the mixture. Suitable binders include starch, gelatin, natural sugars, corn sweeteners, natural and synthetic gums such as acacia, sodium alginate, carboxymethylcellulose, polyethylene glycol, and waxes, or combinations thereof. Lubricants for use in these dosage forms include boric acid, sodium benzoate, sodium acetate, sodium chloride, and the like, or combinations thereof. Disintegrators include, without limitation, starch, methyl-cellulose, agar, bentonite, guar gum, and the like, or combinations thereof. Sweetening and flavoring agents and preservatives can also be included where appropriate. For intravascular, intraperitoneal, subcutaneous, or intramuscular administration, active drug components can be combined with a suitable carrier such as water, saline, aqueous dextrose, and the like. Regardless of the route of administration selected, the compounds of the present invention are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those skilled in the art. The compounds can also be formulated using pharmacologically acceptable base addition salts. Moreover, the compounds or their salts can be used in a suitable hydrated form. Topical formulations of salves and ointments are useful in treating conditions such as psoriasis. Regardless of the route of administration selected, a non-toxic but therapeutically effective quantity of one or more compounds of this invention is employed in any treatment. The dosage regimen for preventing or treating inflammatory conditions with the compounds of this invention is selected in accordance with a variety of factors, including the type, age, weight, sex, and medical condition of the patient, the severity of the inflammatory condition, the route of administration, and the particular compound employed in the treatment. A physician or veterinarian of ordinary skill can readily determine and prescribe the effective amount of the drug required to prevent or arrest the progress of the condition. In so proceeding, the physician or veterinarian could employ relatively low doses at first and subsequently increase the dose until a maximum response is obtained. Daily dosages of the compounds of the invention are ordinarily in the range of about 1 to 50 mg/kg are generally suitable. The accompanying examples illustrate the methods used to prepare the compounds of this invention. Preparation of Starting Material Example A Preparation of ethyl 3-(mercaptomethyl)-1-oxa-4-thiaspiro [4.4]nonane-7-acetate To a stirred solution of ketoester (9.23 g, 0.054 moles) and 2,3-dimercapto-1-propanol (7 g, 0.054 moles) in benzene (75 ml) was added concentrated hydrochloric acid (10 drops). After 3 days at room temperature under a nitrogen atmosphere, the reaction mixture was poured into 5% aqueous K₂CO₃ (100 ml) and extracted with ethyl ether. The organic layer was washed with saturated sodium chloride solution and dried over sodium sulfate. The drying agent was filtered and the filtrate concentrated on the rotary evaporator to give a crude oil (14 g). Chromatography of the crude product on silica gel using 15% ethyl acetate/hexane as the eluent gave 2.1 g of the titled compound as a mixture of four racemates in a ratio of 1.0:1.25:1.25:1.3 as determined by ¹³C nmr. Anal. Calcd. for C₁₂H₂₀S₂O₃: C, 52.16; H, 7.29; S, 23.16. Found: C, 52.31; H, 7.45; S, 23.43. Example B Preparation of ethyl 2-(hydroxymetnyl)-1,4-dithiaspiro [4.4]nonane-7-acetate Continued chromatography using the same solvent system as described in Example A of ethyl 3-(mercaptomethyl)-1-oxa-4-thiaspiro[4.4]nonane-7-acetate gave 6.5 g of the titled compound as a mixture of racemates. Example C Preparation of ethyl 3-(mercaptomethyl)-1-oxa-4-thiaspiro [4.5]decane-7-acetate The titled compound was prepared from the appropriate ketoester (7.3 g, 0.04 mol) and 2,3-dimercapto-1-propanol (5.0 g, 0.04 mol) using the procedure described in Example A. Chromatography of the crude product on silica gel using 30% ethyl acetate/hexane as the eluent gave the titled compound as a mixture of four racemates. Example D Preparation of ethyl 2-(hydroxymethyl)-1,4-dithiaspiro [4.5]decane-7-acetate The titled compound was prepared using the crude product of Example C and the procedure of Example B. Anal. Calcd. for C₁₃H₂₂S₂O₃: C, 53.78; H, 7.63; S, 22.05. Found: C, 53.50; H, 7.86; S, 22.40. Example E Preparation of ethyl 2-(chloromethyl)-1,4-dithiaspiro[4.4] nonane-7-acetate A solution of product of Example B (4.4 g, 0.016 moles) and thionyl chloride (2.3 g, 0.019 moles) in benzene (30 ml) was refluxed under nitrogen for 1 hr. The reaction was cooled and washed with 4% sodium bicarbonate solution and saturated brine and dried (Na₂SO₄). The drying agent was filtered and the filtrate stripped on the rotary evaporator to give an oily residue. The crude product was chromatographed on silica gel using 5% ethyl acetate/hexane to give 4 g of the titled compounds. Anal. Calcd. for C₁₂H₁₉ClS₂O₂: C, 48.90; H, 6.49; S, 21.71. Found: C, 48.65; H, 6.67; S, 21.45. Example F Preparation of ethyl 2-[(acetylthio)methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate A mixture of potassium thioacetate (5.4 g, 0.047 mole) and a solution of the product of Example E (4.0 g, 0.013 mole) in DMF (40 ml) was stirred under nitrogen for 2 days at room temperature. The reaction mixture was diluted with water (200 ml) and extracted twice with ethyl acetate. The organic phase was washed with brine and dried (Na₂SO₄). The drying agent was filtered and the filtrate stripped on the rotary evaporator to give 4.5 g of the titled compound. This material was a single spot on TLC. Example G Preparation of ethyl 2 (mercaptomethyl)-1,4-dithiaspiro-[4.4]nonane-7-acetate A solution of the product of Example F and sodium ethoxide (prepared from 350 mg of sodium metal) in absolute ethanol (40 ml) was stirred for 1 hr under nitrogen. The reaction mixture was poured into 200 ml of 0.5N KHSO₄ and extracted with ethyl acetate. After drying the organic phase over sodium sulfate, the solvent was removed on the rotary evaporator and the residue was chromatographed on silica gel using 5% ethyl acetate/hexane to give 2.1 g of the titled compound. Anal. calcd for C₁₂H₂₀S₃O₂: C, 49.31; H, 6.89; S, 32.84. Found: C, 49.45; H, 6.95; S, 32.67. Example H Preparation of ethyl 2-(chloromethyl)-1,4-dithiaspiro[4.5]decane-7-acetate The titled compound was prepared according to the procedure described in Example E using 6.2 g (0.021 mole) of the product of Example D and 2.75 g (0.025 mol) of thionyl chloride in benzene (60 ml). The crude product was chromatographed on silica gel using 5% ethyl acetate/hexane to give two sets of two racemates. The less polar pair of racemates weighed 0.62 g. The more polar pair of racemates weighed 1.3 g. Example I Preparation of ethyl 2-[(acetylthio)methyl]-1,4-dithiaspiro-[4.5]decane-7-acetate The titled compound was synthesized according to the procedure used for the synthesis of the thioacetate of Example F using the chloride of Example H derived from the less polar pair of racemates (0.62 g, 0.002 mole) and potassium thioacetate (917 mg) in DMF (15 ml). The crude product was chromatographed on silica gel using 10% ethyl acetate/hexane to give 0.57 g of the titled product (Product A.) In a similar manner, chloride of Example H derived from the more polar pair of racemates (1.3 g, 0.0042 mol) was converted to desired thioacetate (0.90 g, Product B). Example J Preparation of ethyl 2-(mercaptomethyl)-1,4-dithiaspiro-[4.5]decane-7-acetate The titled compound was prepared according to the procedure described in Example G using 0.55 g (0.0016 mol) of product A of Example I derived from the less polar pair of racemates of Example H. This product was treated with sodium ethoxide (prepared from 40 mg Na metal in 5 ml of ethanol) at room temperature for 1 hr under N₂. After chromatography of the crude product on silica gel, 400 mg of the titled product was obtained. In a similar manner product B (0.90 g) of Example I (derived from the more polar pair of racemates of Example H) was converted to the titled product (0.70 g). Example K Preparation of ethyl 2-(hydroxymethyl)-1,4-dioxaspiro[4.4]-nonane-7-acetate The ketoester (17.02g, 0.1 mole), glycerol (9.21g, 0.1 mol) and p-toluenesulfonic acid (100 mg) are combined in benzene (150 ml) and refluxed with stirring for 10 hr using a water separator to remove water formed during the ketalization. The reaction mixture is cooled, washed with 5% NaOH solution and water and then dried over sodium sulfate. The drying agent is filtered and the filtrate stripped on a rotary evaporator to give the titled product. The product is purified by chromatography on silica gel using mixtures of ethyl acetate and hexane as eluents. Example L Preparation of ethyl 2-[[[(4-methylphenyl)sulfonyl]oxy] methyl]-1,4-dioxaspiro[4.4]nonane- 7-acetate To a cold (0°) stirred solution of the alcohol of Example K (2.44g, 10 mmol) in pyridine (30 ml) is added p-toluenesulfonyl chloride (1.91g, 10 mmol) in portions over 15 minutes. When addition is completed, the reaction mixture is stirred at room temperature for 2 hrs. The reaction mixture is diluted with ether and washed successively with water, 0.5 N aqueous sodium bisulfate solution, 5% aqueous sodium bicarbonate solution and water. The organic layer is dried over sodium sulfate, filtered, and the filtrate is concentrated on the rotary evaporator to give the titled compound. Example M Preparation of ethyl 2-[(acetylthio)methyl]-1,4-dioxaspiro [4.4]nonane-7-acetate A stirred slurry of potassium thioacetate (1.14 g, 10 mmol) in a solution of the tosylate of Example L (4.14g, 10 mmol) in acetone (30 ml) is refluxed under nitrogen for 5 hrs. The reaction mixture is filtered and the filtrate is concentrated on a rotary evaporator. The residue is dissolved in ethyl acetate, washed with water and dried over sodium sulfate. The drying agent is filtered, the solvent is removed using the rotary evaporated and the residue is chromatographed on silica gel using mixtures of ethyl acetate and hexane as eluents to give the titled compound. Example N Preparation of ethyl 2-(mercaptomethyl)-1,4-dioxaspiro[4.4] nonane-7-acetate Sodium ethoxide is prepared in ethanol (27 ml) using sodium metal (230 mg). Thioacetate of Example M (3.02g, 10 mmol) is added and the reaction mixture is stirred at room temperature under a nitrogen atmosphere for 2 hours. The reaction mixture is poured into 0.5N KHSO₄, extracted with ethyl acetate and dried over Na₂SO₄. The drying agent is filtered, the filtrate concentrated in vacuo and the residue chromatographed on silica gel using mixtures of ethyl acetate and hexane as eluents to give the titled compound. Example O Preparation of ethyl 2-(hydroxymethyl)-1-oxa-4-thiaspiro [4.4]nonane-7-acetate 17.02g (0.1 mol) of the appropriate ketoester and 10.8g (0.1 mol) of 3-mercapto-1,2-propanediol are dissolved in methylene chloride (600 ml). Distilled boron trifluoride etherate (2.5 ml) is added and the reaction is stirred for three days at room temperature under a nitrogen atmosphere. The reaction is quenched by adding 5% aqueous potassium bicarbonate solution (200 ml). The layers are separated and the organic phase is washed with water and dried over magnesium sulfate. The drying agent is filtered and the filtrate is concentrated on a rotary evaporator. The residual oil is chromatographed on silica gel using mixtures of ethyl acetate and toluene as eluents to give the above titled compound. Example P Preparation of ethyl 2-[[[(4-methylphenyl)sulfonyl]oxy] methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate To a cold stirred solution of the product of Example O (2.60g, 10mmol) in pyridine (30 ml) is added p-toluenesulfonyl chloride (1.91g, 10mmol) in portions over 15 minutes. When the addition is completed the reaction is stirred at room temperature for 2 hrs. The reaction mixture is diluted with ether and washed with water, 0.5 N aqueous sodium bisulfate solution, 5% aqueous sodium bicarbonate solution and water. The organic layer is dried over sodium sulfate, filtered, and the filtrate is concentrated on a rotary evaporator to give the titled compound. Example Q Preparation of ethyl 2-[(acetylthio)methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate A stirred slurry of potassium thioacetate (1.14g 10mmol) in a solution of the tosylate of Example P (4.15g, 10mmol) in acetone (30ml) is refluxed under nitrogen for 5 hrs. The reaction mixture is filtered and the filtrate is concentrated on a rotary evaporator. The residue is dissolved in ethyl acetate, washed with water and dried over sodium sulfate. The drying agent is filtered, the solvent is removed using the rotary evaporator and the residue is chromatographed on silica gel using mixtures of ethyl acetate and hexane as eluents to give the titled compound. Example R Preparation of ethyl 2-(metcaptomethyl)-1-oxa-4-thiaspiro [4.4]nonane-7-acetate A solution of sodium ethoxide (prepared from 230 mg of sodium metal) in ethanol (20 ml) and the thioacetate of Example Q (3.18g, 10mmol) is stirred for one hour at room temperature under nitrogen. Ethanol is removed in vacuo on the rotary evaporator and the residue is dissolved in ethyl acetate. This organic phase is washed with 0.5 N sodium bisulfate solution and dried over sodium sulfate. The drying agent is filtered and the filtrate concentrated on a rotary evaporator to give the titled compound. Description of the Preferred Embodiments Example 1 Preparation of ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate A solution of the titled compound of Example A (2.0 g), 7.0 mmol) and 3-(2-n-propyl-3-hydroxy-4-acetylphenoxy)-1-bromopropane (2.3 g), 7.2 mmol), prepared by the method described in U.S. Patent 4,565,882, Example 14, incorporated herein by reference, in methyl ethyl ketone containing suspended potassium carbonate (2.5 g) was refluxed with stirring under N₂ for 15 hrs. The reaction was cooled and filtered and the filtrate concentrated on a rotary evaporator. The residue was chromatographed on silica gel using 20% ethyl acetate/hexane as the eluent to give 3.5 g (95%) of the titled compound. Anal. Calcd. for C₂₆H₃₈O₆S₂: C, 61.16; H, 7.50; S, 12.53. Found: C, 61.09; H, 7.43; S, 12.42. Example 2 Preparation of 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl] thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid A solution of the titled compound of Example 1 ethyl 3-[[[3(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio] methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate (3.0 g, 5.9 mmol) and 2M lithium hydroxide solution (7.5 ml), 2.5 e.q.) in ethanol (35 ml) was stirred overnight at room temperature under N₂. The solvent was removed in vacuo and the residue diluted with water. After acidification to pH 2.5 with 0.5N KHSO₄, the mixture was extracted with ethyl acetate and dried over magnesium sulfate. The drying agent was filtered and the filtrate concentrated in vacuo to give an oil. Further drying of the oil at 60° (0.5 torr) for 4 hr gave 2.58 g (91%) of the titled compound as an oil. Anal. Calcd. for C₂₄H₃₄S₂O₆: C, 59.73; H, 7.10; S, 13.26. Found: C, 59.70; H, 7.30; S, 13.02. Resolution of the titled compound may be accomplished by conventional methods. Example 3 Preparation of ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1-oxa-4-thiaspiro[4.5]decane-7-acetate The titled compound is prepared using the product of Example C and the procedure of Example 1. Chromatography of the crude product on silica gel using mixtures of ethyl acetate/hexane as eluents gives the titled compound. Example 4 Preparation of 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1-oxa-4-thiaspiro[4.5]decane-7-acetic acid Using aqueous 2M LiOH (9 ml) in EtOH 3.2 g (0.0061 mmol) of the product of Example 3 and following the procedure described in Example 2, the titled compound is obtained as an oil. Example 5 Preparation of ethyl 3-[[[2-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate A mixture of K₂CO₃ (2.5 g) and a solution of product of Example G (2.1 g, 7.2 mmol) and bromide (2.25 g, 7.2 mmol) in methyl ethyl ketone was refluxed under N₂ with stirring for 24 hrs. The reaction was filtered, concentrated on a rotary evaporator and the residue chromatographed on silica gel using 30% ethyl acetate/hexane to give 3.5 g (92%) of the titled compound as an oil. Anal. Calcd. for C₂₆H₃₈O₅S₃: C, 59.30; H, 7.27; S, 18.03. Found: C, 59.07; H, 7.50; S, 17.60. Example 6 Preparation of 3-[[[2-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl] thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid The product of Example 5 (3.3 g, 0.0061 mol) was converted to the titled compound using aqueous 2M LiOH (8 ml, 2.5 eq) in ethanol (40 ml) according to the procedure used for the synthesis described in Example 2. After drying the product at 60° (0.5 torr), there was obtained 3.0 g (97% yield) of the titled compound. Anal. Calcd. for C₂₄H₃₄O₅S₃: C, 57.82; H, 6.87; S, 19.28. Found: C, 57.50; H, 6.88; S, 19.51. Example 7 Preparation of ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1,4-dithiaspiro[4.5]decane-7-acetate A mixture of K₂CO₃ (450 mg) and a solution of the product of Example J (derived from product A of Example I) (400 mg, 0.0013 mol) and the bromide compound (411 mg, 0.0013 mol) in methyl ethyl ketone (10 ml) was refluxed with stirring under N₂ for 24 hrs. The reaction was worked up following the procedure described in Example 5. The crude product was purified by chromatography on silica gel using mixtures of ethyl acetate and hexane as eluents to give 650 mg of titled compound (mixture of racemates). The product of Example J (derived from product B of Example I) (0.70 g) was converted to the titled compound (1.23 g) in 94% yield (mixture of racemates). Example 8 Preparation of 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-5β-1,4-dithiaspiro[4,5]decan-7- acetic acid Using aqueous 2M LiOH (2 ml) in EtOH (15 ml) 625 mg (0.0011 mol) of the racemates of Example 7 (ultimately derived from product A of Example I) was converted to the titled product using the same procedure described in Example 1. After drying at 65°C (0.5 torr) for 4 hrs. there was obtained 532 mg of the titled product (ultimately derived from product A of Example I). Anal. Calcd. for C₂₅H₃₆O₅S₃: C, 58.58; H, 7.08; S, 18.73. Found: C, 58.36; H, 7.12; S, 18.63. Using aqueous 2M LiOH (4 ml) in EtOH (30 ml) 1.2 g (0.0022 mol) of the racemates of Example 7 (ultimately derived from product B of Example I) was converted to the titled product using the same procedure described in Example 1. After drying at 65°C (0.5 torr) for 4 hrs. there was obtained 936 mg of the titled product (ultimately derived from product B of Example I). Anal. Calcd. for C₂₅H₃₆O₅S₃: C, 58.58; H, 7.08; S, 18.73. Found: C, 58.54; H, 7.17; S, 18.71. Example 9 Preparation of 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]sulfinyl]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid To a cold (0°C), stirred solution of the sulfide from Example 2 (250 mg, 0.52 mmol) in methylene chloride (2 ml) is added 85% m-chloroperbenzoic acid (106 mg, 0.52 mmol). After stirring 2 hrs. at 0°C, the solvent is evaporated using a nitrogen stream and the residue is chromatographed on silica gel using ethyl acetate containing 0.5% acetic acid as eluent to give the titled compound. Example 10 Preparation of ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-2-hydroxypropyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate 3-(2-n-Propyl-3-hydroxy-4-acetylphenoxy)-1,2-epoxypropane (1.5 g, 0.006 mol) (described in U.S. Patent 4,565,882) is dissolved in 20 ml of methyl ethyl ketone. To this solution is added the mercaptan of Example G (1.75 g, 0.006 mol) and anhydrous potassium carbonate (2.5 g) and the reaction mixture is refluxed with stirring under a nitrogen atmosphere for 24 hrs. The reaction is cooled to room temperature, filtered and the filtrate is concentrated on a rotary evaporator. The residue is chromatographed on silica gel using mixtures of ethyl acetate and hexane as eluents to give the titled compound. Example 11 Preparation of ethyl 3-[[[3-(4-acetyl-3-methoxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4] nonane-7-acetate 3-(2-n-Propyl-3-methoxy-4-acetylphenoxy)-1-bromopropane (1.24 g, 0.037 mol) is dissolved in 15 ml of methyl ethyl ketone containing the mercaptan of Example G (1.08 g, 0.0037 mol). To this solution is added anhydrous potassium carbonate (1.0 g, 7.24 mmol) and the mixture is refluxed for 14 hrs. under an argon atmosphere. The reaction is cooled and filtered and the filtrate is evaporated. The residue is chromatographed on silica gel using mixtures of ethyl acetate and toluene as eluents to give the titled compound. Example 12 Preparation of 3-[[[3-(4-acetyl-3-methoxy-2-propylphenoxy) propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid The product of the above example is saponified according to the procedure described in Example 2 using 0.75 g (0.0016 mol) of the ester of Example 11 and 2M LiOH solution (2.0 ml, 2.5 eq) in ethanol (10 ml). After stirring overnight at room temperature, the solvent is removed in vacuo and the residue diluted with water. After acidification to pH 2.5 with 0.5N KHSO₄, the mixture is extracted with ethyl acetate and dried over magnesium sulfate. The drying agent is filtered and the filtrate is concentrated in vacuo. Further drying at 60° (0.5 torr) gives the titled compound. Example 13 Preparation of ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1,4-dioxaspiro[4.4]nonane-7-acetate A mixture of K₂CO₃ (3.45 g) and a solution of the titled compound of Example N (2.60g, 10 mmol) and 3-(2-n-propyl-3-hydroxy-4-acetylphenoxy)-1-bromopropane (3.15g, 10 mmol) in methyl ethyl ketone (50 ml) is refluxed with stirring under N₂ for 12 hours. The reaction is cooled and filtered. The filtrate is concentrated in vacuo and the oily residue chromatographed on silica gel using mixtures of ethyl acetate and hexane as eluents to give the titled compound. Example 14 Preparation of 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1,4-dioxaspiro[4.4]nonane-7-acetic acid The ester of Example 13 (4.87g, 10mmol) is stirred overnight at room temperature under N₂ with aqueous LiOH solution (2M, 13ml) in methanol (60 ml). The reaction is concentrated on a rotary evaporator and the residue diluted with water. The resulting solution is washed once with ethyl ether and then acidified to pH 2.5 with 0.5N KHSO₄ solution. The aqueous mixture is extracted with ethyl acetate and the organic layer dried over MgSO₄. The drying agent is filtered and the filtrate is concentrated on a rotary evaporator to give an oily residue which is purified by chromatography on silica gel using mixtures of ethyl acetate and hexane containing 0.5% acetic acid as eluents. The purified product is evaporated with methanol and benzene to remove acetic acid. The resulting product is dried on the oil pump (0.1 mm Hg) at 60° to give the titled compound. Example 15 Preparation of ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4] nonane-7-acetate A mixture of K₂CO₃ (3.45g) and a solution of the mercaptan (2.76g, 10mmol) of Example R and 3-(2-n propyl-3-hydroxy-4-acetyl phenoxy)-1-bromo propane (3.15g, 10mmol) in methyl ethyl ketone (50 ml) is refluxed with stirring under N₂ for 12 hrs. The reaction is cooled and filtered. The filtrate is concentrated in vacuo and the oily residue chromatographed on silica gel using mixtures of ethyl acetate and hexane as eluents to give the titled product. Example 16 Preparation of 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy) propyl]thio]methyl]-1-oxa- 4-thiaspiro[4.4]nonane-7-acetic acid The ester of Example 15 (4.87g, 10mmol) is stirred overnight at room temperature under N₂ with aqueous LiOH solution (2M, 13ml) in methanol (60 ml). The reaction is concentrated on a rotary evaporator and the residue diluted with water. The resulting solution is washed once with ethyl ether and then acidified to PH 2.5 with 0.5N KHSO₄ solution. The aqueous mixture is extracted with ethyl acetate and the organic layer dried over MgSO₄. The drying agent is filtered and the filtrate is concentrated on a rotary evaporator to give an oily residue which is purified by chromatography on silica gel using mixtures of ethyl acetate and hexane containing 0.5% acetic acid as eluents. The purified product is evaporated with methanol and benzene to remove acetic acid. The resulting product is dried on the oil pump (0.1 mm Hg) at 60° to give the titled compound. Example 17 Preparation of 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-2-hydroxypropyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid The product of Example 10 is saponified according to the procedure of Example 2 using 5.3g (10 mmol) of the above ester and 2M LiOH solution (12.5 ml, 2.5 eq.) in ethanol (50 ml). After stirring overnight at room temperature, the solvent is removed in vacuo and the residue diluted with water. After acidification to pH 2.5 with 0.5N KHSO₄, the mixture is extracted with ethyl acetate and dried over magnesium sulfate. The drying agent is filtered and the filtrate is concentrated in vacuo. Further drying of the resultant residue at 60° (0.5 torr) gives the titled compound.

Claims: Claims for the following Contracting States : AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE A compound of the formula and the pharmaceutically acceptable salts or geometrical and optical isomers thereof wherein: Alk is alkylene or hydroxyalkylene containing 1-6 carbon atoms; Ar is 5,6,7,8-tetrahydro-1-naphthalenyl or phenyl optionally substituted with one or more substituents selected from alkyl containing 1 to 6 carbon atoms, hydroxy, alkoxy containing 1 to 6 carbon atoms, and alkanoyl containing 2 to 6 carbon atoms; R is hydrogen or alkyl containing 1 to 6 carbon atoms X, Y, and Z are each independently O or S with S optionally oxidized to S=O; m is an integer from 0 to 3; n is an integer from 0 to 5. A compound according to Claim 1, wherein Alk is alkylene containing 1-6 carbon atoms; Ar is phenyl substituted with alkyl containing 1 to 6 carbon atoms, hydroxy and alkanoyl containing 2 to 6 carbon atoms; R is hydrogen or lower alkyl containing 1 to 6 carbon atoms; m is the integer 1 or 2; and n is the integer 1 or 2. A compound according to Claim 2, of the formula and the pharmaceutically acceptable salts and geometrical and optical isomers thereof. A compound according to Claim 3, which is ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]-thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate and isomers thereof. A compound according to Claim 3 which is 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]-methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof. A compound according to Claim 3, which is 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]-methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid and isomers thereof. A compound according to Claim 3, which is ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]-thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof. A compound according to Claim 3 which is 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]-thio] methyl]-1,4-dithiaspiro[4,5]decane-7-acetic acid and isomers thereof. A pharmaceutical composition comprising at least one compound according to Claim 1, together with one or more non-toxic pharmaceutically acceptable carriers. A pharmaceutical composition according to Claim 9 wherein said compound is selected from the group consisting of: Ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate and isomers thereof; 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof; Ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof; 3-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid and isomers thereof; and 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)propyl]-thio]methyl]-1,4-dithiaspiro[4,5]decane-7-acetic acid and isomers thereof. Use of at least one compound of Claim 1 for preparing a medicament for treating allergy. Use according to Claim 11, wherein said compound is selected from the group consiting of: Ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate and isomers thereof; 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4 dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof; Ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof; 3-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid and isomers thereof; and 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl] thio]methyl]-1,4-dithiaspiro[4,5]decane-7-acetic acid and isomers thereof. Use of a compound of Claim 1 for preparing a medicament for treating inflammatory diseases. Claims for the following Contracting State : ES A process for preparing a compound of the formula and the pharmaceutically acceptable salts or geometrical and optical isomers thereof wherein: Alk is alkylene or hydroxyalkylene containing 1-6 carbon atoms; Ar is 5,6,7,8-tetrahydro-1-naphthalenyl or phenyl optionally substituted with one or more substituents selected from alkyl containing 1 to 6 carbon atoms, hydroxy, alkoxy containing 1 to 6 carbon atoms, and alkanoyl containing 2 to 6 carbon atoms; R is hydrogen or alkyl containing 1 to 6 carbon atoms X, Y, and Z are each independently O or S with S optionally oxidized to S=O; m is an integer from 0 to 3; n is an integer from 0 to 5, characterized in that A. a ketoester of the general formula wherein R is alkyl of from 1 to 6 carbon atoms and m and n are as defined above is reacted with 2,3-dimercapto-1-propanol in the presence of a solvent leading to the compound of the formula wherein R, n and m are as defined above, said compound then being reacted with an aryloxy-alkyl bromide Ar-O-Alk-Br with Ar and Alk being as defined hereinabove thus yielding the desired compounds with m and n as defined and with X = S, Z = S and Y = O and R being alkyl of from 1 to 6 cabon atoms, which optionally may be saponified leading to the compounds wherein R is H, or B. reacting the compound of the formula with R = alkyl of 1 to 6 carbon atoms with thionyl chloride and subsequently converting the product into the corresponding mercaptan of the formula with all the substituents as defined hereinabove, said mercaptan then being treated with an appropriate aryloxy-alkyl bromide Ar-O-Alk-Br with Ar and Alk as defined hereinabove thus yielding the desired final compounds wherein X, Y and Z are all S and R being alkyl of from 1 to 6 carbon atoms and m and n as defined before, said compounds optionally being saponified to give the acids wherein R is H, or C. reacting a ketoester of the formula wherein R is alkyl of from 1 to 6 carbon atoms and m and n as defined hereinabove with glycerol in the presence of a solvent to give the compound of the formula with m, n and R as defined above which is converted into its mercaptan of the formula with all the substituents as defined above, said mercaptan subsequently being reacted with an appropriate aryloxy-alkyl bromide Ar-O-Alk-Br with Ar and Alk as defined hereinabove thus yielding the desired final compounds wherein X and Y are O and Z is S and m and n as defined above and R being alkyl of from 1 to 6 carbon atoms, said compounds optionally being saponified to give the corresponding acids wherein R is H, or D. reacting a ketoester of the formula wherein R is alkyl of from 1 to 6 carbon atoms and m and n are as defined hereinabove with 3-mercapto-1,2-propanediol to give the compound of the formula with all the substituents as defined before, said ester then being transformed into the corresponding mercaptan of the formula with m, n and R as defined before, which compound then being reacted with an appropriate aryloxy-alkyl bromide Ar-O-Alk-Br with Ar and Alk as defined before thus yielding the desired final compounds wherein X is O and Y and Z are S and R being alkyl of from 1 to 6 carbon atoms and m and n are as defined before, said esters optionally being saponified to give the corresponding acids wherein R is H, E. converting the desired final compounds, if desired, in a known manner into their pharmaceutically acceptable salts. A process according to Claim 1, wherein Alk of the compound prepared is alkylene containing 1 to 6 carbon atoms; Ar is phenyl substituted with alkyl containing 1 to 6 carbon atoms, hydroxy and lower alkanoyl containing 2 to 6 carbon atoms; R is hydrogen or alkyl containing 1 to 6 carbon atoms; m is the integer 1 or 2; and n is the integer 1 or 2. A process according to Claim 2, wherein the compound prepared is of the formula and the pharmaceutically acceptable salts and geometrical and optical isomers thereof. A process according to Claim 3, wherein the compound prepared is ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]-thio]methyl]-1,4-dithiaspiro[4.4]-nonane-7-acetate and isomers thereof. A process according to Claim 3, wherein the compound prepared is 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof. A process according to Claim 3, wherein the compound prepared is 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]-nonane-7-acetic acid and isomers thereof. A process according to Claim 3, wherein the compound prepared is ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof. A process according to Claim 3, wherein the compound prepared is 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4.5]decane-7-acetic acid and isomers thereof. Use of at least one compound of Claim 1 for preparing a medicament for treating allergy. Use according to Claim 9, wherein said compound is selected from the group consisting of: Ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate and isomers thereof; 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof; Ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof; 3-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid and isomers thereof; and 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl] thio]methyl]-1,4-dithiaspiro[4,5]decane-7-acetic acid and isomers thereof. Use of a compound of Claim 1 for preparing a medicament for treating inflammatory diseases. Claims for the following Contracting State : GR A compound of the formula and the pharmaceutically acceptable salts or geometrical and optical isomers thereof wherein: Alk is alkylene or hydroxyalkylene containing 1-6 carbon atoms; Ar is 5,6,7,8-tetrahydro-1-naphthalenyl or phenyl optionally substituted with one or more substituents selected from alkyl containing 1 to 6 carbon atoms, hydroxy, alkoxy containing 1 to 6 carbon atoms, and alkanoyl containing 2 to 6 carbon atoms; R is hydrogen or alkyl containing 1 to 6 carbon atoms X, Y, and Z are each independently O or S with S optionally oxidized to S=O; m is an integer from 0 to 3; n is an integer from 0 to 5. A compound according to Claim 1, wherein Alk is alkylene containing 1-6 carbon atoms; Ar is phenyl substituted with alkyl containing 1 to 6 carbon atoms, hydroxy and alkanoyl containing 2 to 6 carbon atoms; R is hydrogen or alkyl containing 1 to 6 carbon atoms; m is the integer 1 or 2; and n is the integer 1 or 2. A compound according to Claim 2, of the formula and the pharmaceutically acceptable salts and geometrical and optical isomers thereof. A compound according to Claim 3, which is ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetate and isomers thereof. A compound according to Claim 3 which is 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof. A compound according to Claim 3, which is 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid and isomers thereof. A compound according to Claim 3, which is ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof. A compound according to Claim 3 which is 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]-thio] methyl]-1,4-dithiaspiro[4,5]decane-7-acetic acid and isomers thereof. Use of at least one compound of Claim 1 for preparing a medicament for treating allergy. Use according to Claim 9, wherein said compound is selected from the group consisting of: Ethyl 2-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1,4 dithiaspiro[4.4]nonane-7-acetate and isomers thereof; 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1,4-dithiaspiro[4.4]nonane-7-acetic acid and isomers thereof; Ethyl 3-[[[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetate and isomers thereof; 3-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)propyl]thio]methyl]-1-oxa-4-thiaspiro[4.4]nonane-7-acetic acid and isomers thereof; and 2-[[[3-(4-Acetyl-3-hydroxy-2-propylphenoxy)-propyl]thio]methyl]-1,4-dithiaspiro[4,5]decane-7-acetic acid and isomers thereof. Use of a compound of Claim 1 for preparing a medicament for treating inflammatory diseases.

Inventors: STEALEY MICHAEL ALLAN, WEIER RICHARD MATHIAS, STEALEY, MICHAEL ALLAN, WEIER, RICHARD MATHIAS
Applicants: SEARLE & CO, G.D. SEARLE & CO.
Application year: 1989
Main classification: C07D327
IPC codes: A61K31, C07D327, A61P37, C07D339, A61P43, C07D317, A61P29