NOVEL THROMBOXANE A2 ANTAGONISTS

Abstract: This invention relates to novel optically active thromboxane A₂ inhibiting 7-[3- [1-[[(phenylamino)thioxomethyl]hydrazono]ethyl]-­ bicyclo[2,2,1] heptenoic acids and to methods for their manufacture.

Description: The present invention relates to novel compounds which are pharmacologically useful as thromboxane A₂ antagonist drugs, to pharmaceutical compositions containing the compounds and to methods for preparing them. More specifically, the compounds to which the present invention relates are orally active thromboxane A₂ antagonist agents which promote their effects through their ability to inhibit thromboxane A₂'s powerful induction of platelet aggregation and the platelet release reaction. It is thought that the thromboxane A₂ antagonists exhibit their activity by being able to occupy thromboxane receptor sites. In addition to its platelet aggregation action, thromboxane A₂ has been implicated in other potentially noxious actions on various body systems, including bronchoconstruction and pulmonary and systemic vasoconstriction. Thus thromboxane A₂ may be involved in the normal sealing of blood vessels following injury, but in addition may contribute to pathological intravascular clotting or thrombosis. Moreover, the constrictor actions of thromboxane A₂ on bronchiolar, pulmonary vascular and systemic vascular smooth muscle may be important in the development of several anaphylactic conditions, including bronchial asthma. There is also some evidence to implicate thromboxane A₂ in the pathogenesis of inflammation. Thromboxane A₂ antagonists of the present invention belong to a group of bicycloheptenoic acids as partially exemplified in European Patent 0043292, United States Patent No. 4,596,823 and Japanese Patent Application No. 81/502230 to Jones et al which specifically describe the preparation of the optically inactive racemate 7-[3α-[1-[[(phenylamino)thioxomethyl]hydrazono]ethyl]-1R,1S,1α,4α-bicyclo [2.2.1]hept-2β-yl]-5Z-heptenoic acid. These documents contain a general discussion of the various modes of representing the different isomers of such disubstituted bicyclic compounds. Accordingly the present invention comprises a compound being 7-[3α-[1-[[(phenylamino) thioxomethyl]hydrazono]ethyl]-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid and having the structure and a compound being 7-[3α-[1-[[(phenylamino) thioxomethyl]hydrazono]ethyl]-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid and having the structure and pharmaceutically acceptable salts thereof. The present invention further includes intermediate compounds being 7-[3α-acetyl-1R,1α,4α-bicyclo[2.2.1]-hept-2β-yl]-5Z-heptenoic acid and having the formula and 7-[3α-acetyl-1R,1α,4α-bicyclo[2.2.1]-hept-2β-yl]-5E-heptenoic acid and having the formula To the extent that the biologically active and intermediate compounds described herein may also be prepared as salts, such forms are included in the present invention. Typical of "pharmaceutically acceptable salts" are those non-toxic salts such as salts in which the cationic portion is taken from the alkali metals, especially sodium and potassium, from the alkaline earth metals, especially calcium, and the ammonium cation. Various processes for the preparation of the compounds described above and analogues thereof will now be described, these processes also being included by the present invention. A particular process of the present invention for making the compounds of the invention, namely or pharmaceutically acceptable salts thereof, comprises the step of resolving a compound of the formula into the respective enantiomers of the formulae by reacting the compound of formula (VIII) with a diol whose hydroxyl functions are attached to adjacent carbon atoms. As indicated, the diol is one whose hydroxyl functions are attached to adjacent carbon atoms, but especially is one in which the adjacent chiral carbon atoms have symmetry elements that define C₂ symmetry as defined hereinafter, and most preferably the diol is (2R,3R)-2,3-butanediol or (2S,3S)-2,3-butanediol. "C₂ symmetry" means that a molecule possesses a simple twofold axis as illustrated: See W. J. LeNoble, Highlights of Organic Chemistry, pages 161/162, Marcel Dekker (1974). Preferably this particular process of the invention comprises (a) reacting a compound of the formula with(2R,3R)-2,3-butanediol or (2S,3S)-2,3-butanediol; (b) hydrolyzing the product of (a); (c) oxidizing the product of (b) by peracid oxidation; (d) reducing the product of (c); and (e) reacting the product of (d) with (4-carboxybutyl)triphenylphosphonium bromide followed by oxidation to produce a ketone which is isomerized with base to the stable isomer; (f) reacting the ketone product of (e) with 4-phenyl-3-thiosemicarbazide; and where appropriate (g) forming pharmaceutically acceptable salts of the product of (f). Another particular process of the present invention for making compounds of the formula which can be either the cis or the trans double bond geometric isomer, or a pharmaceutically acceptable salt thereof comprises the steps of reacting a compound of the formula in the presence of a base to form an anion of the formula which is then alkylated to form a compound of the formula wherein R¹ is methyl. More specifically, this method comprises the steps of (a) reacting a compound of the formula with a triflating agent in the presence of a base to form a compound of the formula which is then alkylated to form a compound of the formula wherein R¹ is methyl; (b) hydroborating and oxidizing the product of (a); (c) oxidizing the product of (b) by peracid oxidation; (d) reducing the product of (c); (e) reacting the product of (d) with (4-carboxybutyl)triphenylphosphonium bromide followed by oxidation; and (f) reacting the product of (e) with 4-phenyl-3-thiosemicarbazide; and where appropriate (g) forming a pharmaceutically acceptable salt of the product of (f). A particularly important group of intermediate compounds of use in preparing the compounds of the present invention include: 7-[3α-Acetyl-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid: and 7-[3α-Acetyl-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid: Other intermediate compounds of use in preparing the compounds of the present invention are those of the formula (III): wherein is a heterocyclic ring of a structure selected from the group consisting of wherein R¹ is methyl. Especially preferred compounds of formula (III) are as follows: 4aα,8aα-Octahydro-1R,1α-methyl-5α,8α-methano-1H-2-benzopyran-3-ol: 4aα,8aα-Octahydro-1R,1α-methyl-5α,8α-methano-3H-2-benzopyran-3-one: 4aβ,8aβ-Octahydro-1R,1α-methyl-5β,8β-methano-1H-2-benzopyran-3-ol: and 4aβ,8aβ-Octahydro-1R,1α-methyl-5β,8β-methano-3H-2-benzopyran-3-one: Other intermediate compounds of the present invention are those of the formula (IV): wherein R¹, R² or R³ are independently hydrogen, methyl or a keto group, with the proviso that only one keto group may be present on the 'b' ring. Especially preferred compounds of the formula (IV) are as follows: 3aR,3aα,7aα-Octahydro-4α,7α-methano-1H-inden-1-one: 3aα,7aα-Octahydro-1R,1α-methyl-4α,7α-methano-2H-inden-2-one: 3aβ,7aβ-Octahydro-1R,1α-methyl-4β,7β-methano-2H-inden-2-one: and 3aα,7aα-Octahydro-1S,1α-methyl-4α,7α-methano-2H-inden-2-one: Other intermediate compounds of use in preparing the compounds of the present invention are those of the formula (V): wherein R¹ and R² are independently hydrogen, methyl or trifluoromethylsulfonyloxy; and which can be saturated or unsaturated in the 'b' ring, with the proviso that there can be no more than one degree of unsaturation in the 'b' ring. Especially preferred compounds of the formula (V) are as follows: 3aS,3aα,4,5,6,7,7a-Hexahydro-4α,7α-methano-1H-indene: 3aS,3aα,4,5,6,7,7aα-Hexahydro-3-methyl-4α,7α-methano-1H-indene: 3aS,3aα,4,5,6,7,7aα-Hexahydro-4α,7α-methano-1H-inden-3-yl trifluoromethanesulfonate: 3aβ,7aβ-Octahydro-1R,1α-methyl-2β-hydroxy-4β,7β-methano-2H-indene: Other intermediate compounds of use in preparing the compounds of the present invention are those of the formula (VI): wherein R¹, R², R³ and R⁴ are independently hydrogen or methyl. Especially preferred compounds found of the formula (VI) are as follows: 3'aα,7'aα-Octahydro-1'R,1'α,4S*,5S*-trimethyl-spiro[1,3-dioxolane-2,2'-[4'α,7'α]methano-[2H]indene]: 3'aα,7'aα-Octahydro-1'S,1'α,4R*,5R*-trimethyl-spiro[1,3-dioxolane-2,2'-[4'α,7'α]methano-[2H]indene]: The present invention also includes novel pharmaceutical compositions comprising one or more of the active compounds of the invention in combination with suitable pharmaceutical diluents or carriers. In the pharmaceutical compositions of the present invention, the foregoing compounds described in detail above will form the active ingredients and will typically be administered in admixture with suitable pharmaceutical diluents or carriers, including excipients, suitably selected with respect to the intended form of administration, for example oral tablets, capsules, elixirs, syrups and the like, and consistent with conventional and pharmaceutical practices. For instance, for oral administration in the form of tablets or capsules, the active drug components may be combined with an oral non-toxic pharmaceutically acceptable inert carrier such as lactose, starch, sucrose, glucose, methyl cellulose, magnesium stearate, dicalciumphosphate, calcium sulphate, mannitol, sorbitol and the like; for oral administration in liquid form, the active drug components may be combined with any oral non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents and coloring agents can also be incorporated in the mixture. Suitable binders include starch, gelatin, natural sugars such as glucose or β-lactose, corn sweeteners, natural and synthetic gum such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol and waxes. Lubricants for use in these dosage forms include boric acid, sodium benzoate, sodium acetate, sodium chloride and the like. Disintegrators include, without limitation, starch, methylcellulose, agar, bentonite, xanthan gum and the like. In addition, the compounds can be used in in vitro diagnosis (e.g. in assays for thromboxane A₂ and the like). "Pharmacologically effective amount" means a dosage or dosage regimen utilizing the compounds of the present invention that has been selected in accordance with a variety of factors including the type, species, age, weight, sex and medical condition of the patient; with the severity of the condition to be ameliorated; the route of administration; the renal and hepatic function of the patient; and the particular compound employed or mixtures thereof. An ordinarily skilled veterinarian or physician can readily determine and prescribe the effective amount of the drug required to prevent, treat or arrest the progress of the condition. As indicated the compounds of the present invention can be administered in such oral dosage forms as tablets, capsules, pills, powders, granules, elixirs, tinctures, suspensions, syrups, emulsions, shake solutions, colloids or suspensions. Likewise, they may also be administered in intravenous, intraperitoneal, subcutaneous or intramuscular form, all using forms known to those of ordinary skill in the pharmaceutical arts. In general, the preferred form of administration is oral. Dosages of the compound of the present invention, when used for the indicated effects, will range between about 0.01 mg/kg to 100 mg/kg and preferably 0.1 mg/kg to 10 mg/kg, Advantageously, the compounds of the present invention may be administered in a single daily dose or the total daily dosage may be administered in equal divided dosages of two, three or four times daily. As has been indicated previously, the thromboxane A₂ antagonist compounds of the present invention are optically active, i.e. the compound is present in a greater than 1:1 proportion relative to its enantiomer. More preferably the compound is substantially free from its enantiomer, for example one enantiomer being present as at least 80% by weight and especially at least 90% by weight of the total of both enantiomers. It will be appreciated that the optically active compounds are selected on the basis of an advantage of any form for pharmaceutical use, such as greater thromboxane A₂ antagonism, lower undesirable side effects or greater suitability in formulation or administration due to their particular physical properties, for example by virtue of greater solubility. The properties of the optically active compounds of the present invention are illustrated hereinafter in the Examples. The compounds of the invention are readily prepared according to one of the following reaction schemes or modifications thereof using readily available starting materials, reagents and conventional procedures for the individual steps of the schemes. Although the methods of the present invention as defined herein are novel they do utilise individual steps which are known in other contexts and the reaction conditions used may therefore conveniently be those known to be suitable for the reaction in question. In these reactions it is, however, also possible to make use of variants which are in themselves known but are not mentioned herein in detail. The following examples further illustrate details of the various methods for the preparation of the compounds of the invention. All temperatures are degrees Celsius unless otherwise noted. Those skilled in the art will readily understand that known variations of the conditions of the following preparative procedures can also be used to prepare these compounds. EXAMPLES SYNTHESIS OF SC-46984 Example 1 (±)3aα,7aα-Octahydro-1α-methyl-4α,7α-methano-2H-inden-2-one, (b). To a solution of 6.9 mL (5.93 g, 41.9 mmole) of N-isopropylcyclohexyl-amine in 20 ml of dry THF cooled in a -78° bath was added 25 ml of 1.58 m n -butyllithium in hexane. After 15 min a solution of 5.62 g (37.5 mmole) of (a) in 20 ml of THF was added over 20 minutes. This mixture was stirred 15 minutes and 5 ml (11.4 g, 80 mmole) of methyl iodide was added. After 30 minutes more, the mixture was allowed to warm to room temperature and 50 ml water was added. The mixture was extracted twice with ether, washed with water and brine and then dried over sodium sulfate. The solvents were evaporated and the residue was chromatographed (Flash, hexane-EtOAc 99:1) to provide first 596 mg (9%) of 3aα,7aα-octahydro-1α,3α-dimethyl-4α,7α-methano-2H-inden-2-one, (c), followed by 3.81 g (62%) of (b), and then a small amount of crude (a). In some runs the early part of the (b) fraction contained (±)3aα,7aα-octahydro-1,1-dimethyl-4α,7α-methano-2H-inden-2-one, (d). In a run which was allowed to stand at room temperature overnight before workup, a small amount of the isomeric (±)3aβ-octahydro-1α-methyl-4β,7β-methano-2H-inden-2-one, (e), followed the main product closely. This isomer also forms under acidic equilibration. A solution of 2.26 g (13.8 mmole) of (b), 1.07 g (11.9 mmole) of (2 R ,3 R )-2,3-butanediol and 10 mg of camphorsulfonic acid in 40 ml of benzene was refluxed under a Dean-Starke trap for 12 hours and then distilled to a volume of ca. 10 ml. After the addition of a few drops of Et₃N and concentration in vacuo , the residue was chromatographed (Flash, hexane - hexane-EtOAc 49:1) to provide 1.95 g of a product fraction followed by 430 mg of (b) enriched in the l-enantiomer and then by 66 mg of (e) enriched in the d-enantiomer. The product fraction was repeatedly chromatographed (LPLC Woelm, hexane-Et₂O 49:1) cutting small fractions arbitrarily and combining those with >90% diastereomeric purity. The major isomer was 3'aα,7'aα-octahydro-1's,1'α,4S*-5S*-trimethyl-spiro[1,3-dioxolane-2,2'-[4'α,7'α]methano[2H]indene], (p), which was also the faster moving eventually provided 851 mg (92% (p) and 8% 3'aα,7'aα-octahydro-1'R,1'α,4R*,5R*-trimethyl-spiro[1,3-dioxolane-2,2'-[4'α,7'α]methano[2H]indene], (q), based on ¹³C NMR). The 430 mg of recovered (b), 300 mg of (2S,3S)-2,3-butanediol and 5 mg of camphorsulfonic acid in 35 ml of benzene was refluxed under a Dean-Starke trap for 16 hours. After cooling, adding Et₃N and evaporating in vacuo , Flash Chromatography as above provided 636 mg of product fraction followed by a mixture of 48 mg of (b) and (e) isomers. LPLC of the product fractions as above provided 271 mg of the faster moving fractions consisting of 95% 3'aα,7'aα-octahydro-1'R,1'α,4S*,5S*-trimethyl-spiro[1,3-dioxolane-2,2'-[4'α,7'α]methano-[2H]indene], (r), and 5% 3'aα,7'aα-octahydro-1'S,1'α,4R*,5R*-trimethyl-spiro[1,3-dioxolane-2,2'-[4'α,7'α]-methano-[2H]indene], (p). The NMR analysis was based on the relative peak heights for a methylene carbon at ca. 35.4 ppm in isomers (p) and (r) vs. 34.7 ppm in isomers (q) and (s), and a methyne carbon at ca. 39.2 ppm in isomers (p) and (r) vs. 39.7 ppm in isomers (q) and (s). Example 2 3aα,7aα-Octahydro-1R,1α-methyl-4α,7α-methano-2H-inden-2-one, (-b). The 271 mg (95% r) was dissolved in 15 ml of MeOH and a solution of 400 mg of oxalic acid dihydrate in 5 ml of water was added. After stirring at room temperature for 18 hours, the mixture was diluted with water and extracted 3X hexane, washing with 5% NaHCO₃, water and brine. After drying over sodium sulfate and evaporation the residue was chromatographed (Flash, hexane-EtOAc 19:1) to provide 154 mg (82%, 90% ee) of (-b), [α] D -21.2° (0.998% hexane). Example 3 4aα,8aα-Octahydro-1R,1α-methyl-5α,8α-methano-3H-2-benzopyran-3-one, (+f). To a solution of 132 mg (0.8 mmole) of (-b) in 4 ml of dry methylene chloride was added 210 mg (178 mg, 1.03 mmole) of 85% m -chloroperoxybenzoic acid. After two days the solids were removed by filtration and rinsed with hexane. The filtrate was washed with sodium bicarbonate, water and brine. After evaporation of solvents chromatography (Flash, hexane-EtOAc 98:2) provided 132 mg (91%) of (+f). Crystallization from a small amount of hexane provided solid melting at 43-44°, [α] D +105.1° (0.969% CH₂Cl₂). Example 4 4aα,8aα-Octahydro-1R,1α-methyl-5α,8α-methano-1H-2-benzopyran-3-ol, (+g). A solution of 95 mg (0.52 mmole) of (+f) in 5 ml of toluene was chilled in a -78° bath and 2 ml of 1 M DIBAL in toluene was added and stirred at -78° for 2 hours. The mixture was quenched cautiously with 1 ml of MeOH. After warming to room temperature, 20 ml more MeOH was added. The solid was removed by filtration, rinsing thoroughly with MeOH. The filtrate was evaporated, and the residue was chromatographed (Flash, hexane-EtOAc 3:2) to provide 74 mg (77%) of (+g), [α] D +34.8° (0.705% hexane). Example 5 7-(3α-Acetyl-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl)-5Z-heptenoic acid, (-n). To a suspension of 1.0 g (2.31 mmole) freshly crushed and dried (60°, high vac) (4-carboxybutyl)triphenylphosphonium bromide in 5 ml dry THF was added 4.6 ml of 1 M sodium (bistrimethylsilyl)-amide in THF. The mixture was stirred at room temperature for 1 hour under nitrogen, cooled in an ice bath and then a solution of 60 mg (0.33 mmole) of (+g) in 5 ml of THF was added. The ice bath was removed and the mixture was stirred for 2 hours. After the addition of 25 ml of 5% NaHCO₃ the mixture was extracted with ether. The aqueous layer was acidified with 10% HCl and extracted twice with ether washing with water and brine. After being dried over sodium sulfate the solvents were evaporated and the residue was chromatographed on a short acidic silica column (hexane-EtOAc, 4:1) to provide 70 mg of a crude product which was dissolved in 10 ml of acetone, chilled in an ice bath and treated with a slight excess of Jones reagent. The mixture was diluted in 20 ml of water and extracted with ether. After washing with water and brine followed by drying over sodium sulfate the solvents were evaporated and the residue was dissolved in 1 ml of 1 N NaOH and stirred at room temperature for 1 hour. After acidification with 10% HCl the product was extracted with ether which was washed with water and brine and dried over sodium sulfate. After evaporating solvents chromatography on a short acidic silica column (hexane-EtOAc 4:1) provided 47 mg (53%) of a mixture containing ca. 90% (-n) and 10% (p). Example 6 7-[3α-[1-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-1R, 1α,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid, SC-46984. A solution of 47 mg (0.18 mmole) of the mixture of (-n) and (p) and 60 mg (0.36 mmole) of 4-phenyl-3-thiosemicarbazide in 0.5 ml of pyridine was stirred at room temperature for 28 hours. A solution of the mixture in 20 ml of methylene chloride was washed twice 20 ml 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on an acidic silica column (hexane-EtOAc, 4:1) to provide 72 mg, (97%) of (-r) (90% ee) containing about 10% of its 5E isomer, (-s). CHIRAL TEMPLATE BASED SYNTHESIS Example 7 3aR,3aα,7aα-Octahydro-4α,7α-methano-1H-inden-1-one, (+v). A solution of 5.65 g of 3aS,3aα,4,7,7aα-tetrahydro-4α,7α-methano-1H-inden-1-one², (+u), in 50 ml THF was hydrogenated over a small amount of W-2 Raney Nickel at atmospheric pressure and 25°C over 2 hours. After removing the catalyst and distillation of the solvent Flash chromatography (hexane-ether 4:1) provided 5.21 g (90%) of (+v), a waxy solid, [α] D +260.5° (0.925% hexane) followed by a small amount of an over reduction product. Example 8 3aS,3aα,4,5,6,7,7aα-Hexahydro-4α,7α-methano-1H-inden-3-yl, trifluoromethanesulfonate, (-w) by the method of McMurray and Scott (References 3 and 4). To a solution of 6.2 ml (5.32 g, 37.7 mmoles) of N-isopropylcyclohexylamine in 30 ml of THF at -78°C was added 26.45 ml (37.8 mmoles) of 1.43 M N-butyllithium in hexane. After stirring for 45 minutes, a solution of 5.11 g (34.1 mmoles) of (+v) in 25 ml THF was added. After 2 hours at -78°C a solution of 13.51 g (37.8 mmoles) of N-phenyltrifluoromethanesulfonimide in 40 ml THF was added and the mixture was stirred at 0° for 16 hours. After the addition of 50 ml water, the product was extracted with hexane washing with water and brine. After evaporating solvents the residue was chromatographed (Flash, hexane) to provide 5.71 g of crude (-w), [α] D -14.6° (1.012% hexane). Example 9 3aS,3aα,4,5,6,7,7aα-Hexahydro-3-methyl-4α,7α-methano-1H-indene, (y) by the method of McMurray and Scott (References 3 and 4). To a slurry of 7.26 g (38.1 mmoles) of copper iodide in 25 ml ether at -78° was added 64.6 ml (76.2 mmoles) of 1.18 M methyllithium-lithium bromide complex in ether. After warming to -30° a solution of 9.0 g (31.8 mmoles) (-w) in 5 ml of ether was added. The mixture was maintained at -20° for 20 hours and then 5 ml of methyl iodide was added. After being warmed to 20°, 100 ml saturated NH₄Cl and a small amount of NH₄OH were added. After being stirred at room temperatures for 20 hours, the mixture was extracted with hexane, washed with water and brine, and dried over sodium sulfate. After concentrating the solution, chromatography (Flash, hexane) provided 4.17 g of a mixture of (y) and 3aS,3aα,4,5,6,7,7aα-hexahydro-4α,7α-methano-1H-indene, (x), (4:1). Example 10 3aβ,7aβ-Octahydro-1R,1α-methyl-2β-hydroxy-4β,7β-methano-2H-indene, (z). To a solution of 4.17 g of the crude mixture of (y) and (x) in 30 ml THF at 0° was added 20 ml of 2M borane-methyl sulfide complex in THF. The mixture was allowed to warm to room temperature and stirred for 3 hours. After again cooling to 0°, 10 ml water and 40 ml 1N NaOH were added followed by 20 ml 30% hydrogen peroxide. The mixture was allowed to warm to room temperature and stirred for 45 minutes more. After the addition of 50 ml 20% sodium carbonate solution the products were extracted with ether, washing with water and brine. After drying over sodium sulfate, solvents were evaporated and the residue amounted to 5.16 g of crude (z). Example 11 3aβ,7aβ-Octahydro-1R,1α-methyl-4β,7β-methano-2H-inden-2-one, (+aa). The 5.16 g of crude (z) (above) was dissolved in 50 ml acetone and chilled in an ice bath. Jones reagent was added until a slight excess persisted (ca. 10 ml). After dilution with 200 ml water, the mixture was extracted with ether, washing water, 5% sodium bicarbonate and brine. After removing solvents chromatography (Flash, hexane-ether 98:1) provided 2.35 g of (+aa), [α] D +20.2° (1.107% hexane), as the first material eluted. This was followed by a mixture of (v) and (a). Preparation of a ketal with (2 R ,3 R )-2,3-butanediol as above provided a sample which contained only ketal (q) by ¹³C NMR analysis. Example 12 4aβ,8aβ-Octahydro-1R,1α-methyl-5β,8β-methano-3H-2-benzopyran-3-one, (bb). A solution of 1.53 g (9.4 mmoles) of (+aa) and 2.47 g (12 mmoles) 85% m -chloroperoxybenzoic acid in 20 ml methylene chloride was stirred at room temperature for 5 days. The solid was removed by filtration, rinsing well with hexane. The filtrate was evaporated and the residue chromatographed (Flash, hexane-EtOAc 9:1 - 4:1) to provide 0.38 g (25%) of recovered (+e) followed by 0.74 g (44%) of (bb). Example 13 4aβ,8aβ-Octahydro-1R,1α-methyl-5β,8β-methano-1H-2-benzopyran-3-ol, (cc). To a solution of 740 mg (4.11 mmoles) of (bb) in 5 ml toluene at -78° was added 10.3 ml 1M diisobutylaluminum hydride in toluene. After 4 hours at -78°, 30 ml MeOH was added and the mixture was allowed to warm to room temperature. After 2 hours the solids were removed by filtration rinsing thoroughly with MeOH. The filtrate was evaporated and the residue chromatographed (Flash, hexane-EtOAc 7:3) to provide 620 mg (83%) of (cc). Example 14 7 - [3β-(1S*-Hydroxyethyl)-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid, (dd) and 7-[3β-(1S*-hydroxyethyl)-1R,1α,4α-bicyclo-[2.2.1]hept-2β-yl]-5E-heptenoic acid, (ee). To a suspension of 3.02 g (6.8 mmoles) (4-carboxybutyl)triphenylphosphonium bromide in 20 ml THF was added 13.6 ml 1M sodium bis(trimethylsilyl) amide in THF. After 18 hours stirring at room temperature, the mixture was cooled in an ice bath and a solution of 0.62 g (3.4 mmoles) of (cc) in 20 ml THF was added. After 4 hours the mixture was allowed to warm to room temperature for 20 hours more and then 20 ml water was added. The aqueous layer was separated and acidified with 10% HCl. The products were extracted with ether, washing with water and brine. After drying over sodium sulfate and evaporation, repeated low pressure liquid chromatography (Woelm pH controlled silica, hexane-EtOAc 3:2) provided first 70 mg (8%) of (ee) followed by 400 mg (44%) of (dd). Example 15 7-(3α-Acetyl)-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl)-5Z-heptenoic acid, (-n). A solution of 400 mg (1.5 mmoles) of (dd) in 12 ml acetone was chilled in an ice bath and about 1 ml of Jones reagent was added until a slight excess persisted. After dilution with 20 ml water extraction with ether, washing with water and brine and drying over sodium sulfate, evaporation of solvents left a residue of 380 mg. This material was dissolved in 15 ml 1N NaOH. After 1 hour at room temperature the solution was acidified with 10% HCl and extracted with ether washing with water and brine. Chromatography on acidic silica (hexane-EtOAC 4:1) provided 160 mg (40%) of (-n). Example 16 7-(3α-Acetyl)-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl)-5E-heptenoic acid, (gg). A solution of 70 mg (0.26 mmole) of (ee) in 3 ml acetone was chilled in an ice bath and titrated with Jones reagent (ca. 0.08 ml). After dilution with water and ether extraction, washing with water and brine, drying over sodium sulfate and evaporation left a residue of 80 mg. This material was dissolved in 1.5 ml 1N NaOH. After 1 hour at room temperature the solution was acidified with 5% HCl and the product extracted with ether washing with water and brine. After drying over sodium sulfate and evaporation of solvents, chromatography on acidic silica gave 40 mg (58%) of (gg). Example 17 7-[3α-[1-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-1R, 1α,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid, SC-46984. A solution of 140 mg (0.53 mmole) of (-n) and 106 mg (0.64 mmole) of 4-phenyl-3-thiosemicarbazide in 0.6 ml of pyridine was stirred at room temperature for 20 hours. A solution of the mixture in 20 ml of methylene chloride was washed twice 20 ml 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on acidic silica column (hexane-EtOAc 4:1) to provide 160 mg, (73%) of SC-46984. Example 18 7-[3α-[1-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-1R, 1α,4α-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid, SC-47721. A solution of 40 mg (0.15 mmole) of (gg) and 30 mg (0.18 mmole) of 4-phenyl-3-thiosemicarbazide in 0.4 ml of pyridine was stirred at room temperature for 20 hours. A solution of the mixture in 20 ml of methylene chloride was washed twice 20 ml 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on acidic silica column (hexane-EtOAc 4:1) to provide 40 mg, (64%) of SC-47721. References 1. Brown, H. C.; Rothberg, I.; Vander Jagt, D. L., J. Org. Chem . 1972, 37 , 4098-4100. 2. Klunder, A. J. H.; Huizinga, W. B.; Hulshof, A. J. M.; Zwanenburg, B., Tetrahedron Letters 1986, 27 , 2543-2546. 3. McMurray, J. E.; Scott, W. J., Tetrahedron Letters 1983, 24 , 979-982. 4. McMurray, J. E.; Scott, W. J., Tetrahedron Letters 1980, 21 , 4313-4316. Notes for the Pharmacological Data Compound SC 44161 is (±)7-[3α-[1-[[(phenylamino)thioxomethyl]hydrazono]ethyl]-1α,4α-bicyclo [2.2.1] hept-2β-yl]-5Z-heptenoic acid. Compound SC 46985 is 7-[3α-[1-[[(phenylamino)thioxomethyl]hydrazono]ethyl]-1S,1α,4α-bicyclo [2.2.1] hept-2β-yl]-5Z-heptenoic acid. Compound SC 46986 is (±)7-[3α-[1-[[(phenylamino)thioxomethyl]hydrazono]ethyl]-1α,4α-bicyclo [2.2.1] hept-2β-yl]-5E-heptenoic acid. Pharmacologic Activity Methods: I. In vitro a. Inhibition of U-46619-induced platelet aggregation (rat) Platelet rich plasma (PRP) was prepared from heparanized whole blood (5 units/ml) drawn from the abdominal aorta of ether anaesthetized rats. PRP was prepared by centrifugation (Sorvall RC2B, DuPont, Wilmington, Del.) of the whole blood at 150 x g for 10 minutes. Platelet poor plasma (PPP) was prepared by centrifugation at 1200 x g for 20 minutes. Aliquots of PRP and PPP were used to standardize the aggregometer (Payton, Buffalo, N.Y., model 300B) with the recorder (Riken Denshi 2 channel recorder, Payton, model SP-H5P) for measurement of minimum and maximum light transmittance, respectively. Platelet aggregation was measured as an increase in light transmittance. PRP (390 µl aliquot + 5 µl compound vehicle) was preincubated for 1 minute at 37°C, with stirring at 900 rpm in the aggregometer. The vehicle control response was obtained by adding U-46619 (Cayman Chemical, Ann Arbor, MI, 1-2 units/cuvette/10 µl) to the aliquot of PRP. Aggregation was monitored until maximum aggregation was achieved (approximately 3 minutes following the addition of U-46619). Amount of aggregation was measured by grids on the strip chart. Compounds were dissolved in either ethanol (EtOH) or Na₂CO₃ (0.1 M) and preincubated at the desired concentration in PRP for 1 minute at 37°C with stirring. At the end of the preincubation, U-46619 was added and aggregation measured. Inhibition of aggregation by compound was calculated as percent inhibition of the control aggregation response to U-46619. Dose response curves were generated and IC₅₀s calculated by linear regression. b. Analysis of partial agonism with SC-46985 (human washed platelets) Sixty ml of blood were drawn into 1/10 volume of CCD (100 mM Na Citrate and 136 mM glucose, pH 6.5 with HCl) and PRP prepared as described above. PRP was placed on ice for 15 minutes. One half of the volume of CCD was added and recentrifuged. The platelet pellet was gently resuspended in 1/2 the original volume of PRP in a 0°C modified Tangen-Hepes-BSA buffer (145 mM NaCl, 5 mM KCl, 0.05 mM CaCl₂, 0.1 mM MgCl₂, 11 mM glucose, 15 mM Hepes, 1 mg/ml bovine serum albumin, pH adjusted to 7.4 with NaOH). The resuspended platelets were incubated undisturbed at 37°C for 30 minutes. After incubation a platelet count was determined and adjusted to 3 x 10⁸ cells/ml with the Tangen-Hepes-BSA buffer. 425 µl of the washed human platelet suspension was incubated in the aggregometer in the presence of 16 mM CaCl₂ (50 µl) and SC-46985 for 5 and 10 minutes. Baseline aggregation was observed to determine any compound related shape change. (In a study similar to (a) above, aliquots of human washed platelets were incubated with various concentrations of SC-46985 and U-46619 to determine dose-related inhibition of aggregation). c. Inhibition of collagen induced platelet aggregation in dogs by SC-44161 and SC-46984 Blood was collected into citrated vacutainers from the jugular veins of conscious dogs and centrifuged at 200 x g for 7 minutes. PRP was separated and an aliquot (450 µl) incubated 1 minute in the aggregometer (as described above). Collagen (50 µl, Helena Reagent, bovine tendon) was added and aggregation monitored for 5 minutes. Percent inhibition and IC₅₀s were determined as previously described. II. Ex vivo - (rat) Inhibition of U-46619 induced platelet aggregation a. Intravenous Rats were anaesthetized with ether and the jugular vein exposed. Compound dissolved in 0.1 M Na Carbonate was injected as a bolus into the jugular vein. Blood (5 ml, heparanized) was sampled 30 seconds later from the abdominal aorta. PRP was prepared by quick centrifugation (Eppendorf, model 5415 at 12,000 rpm for 5 seconds). PPP was prepared by centrifugation for 2 minutes. PRP was diluted 1:1 with PPP from similarly treated animals. Aggregation was monitored for 5 minutes and inhibition was determined as previously described. b. Intragastric Compounds dissolved in Na Carbonate were administered to conscious rats with an I.G. tube. Fifteen minutes post administration (time previously determined to show maximum effect), rats were anaesthetized with ether and blood was sampled from the abdominal aorta. PRP was prepared as described in IIa. Results: I. In vitro a. Inhibition of U-46619 induced platelet aggregation (rat) Results of in vitro studies in rat PRP with the compounds in two vehicles, EtOH and Na Carbonate, are reported in Table I. SC-44161 (racemic) was 5-10 fold more potent when in aqueous solution than when dissolved in ethanol. SC-46984, the cis isomer of the active enantiomer, was 10-50 times more potent in aqueous solution than in ethanol, and approximately 100 times more potent than SC-44161. The trans form of the active enantiomer, SC-47721, was less potent than SC-46984 but still 10-50 times more potent than SC-44161. b. Analysis of partial agonism with SC-46985 (human) SC-46985 was incubated with washed human platelets in an attempt to detect potential partial agonist activity. After 5 and 10 minutes incubation, there was no evidence of shape change or aggregation, indicating a lack of partial agonist activity. (When aliquots of platelets were incubated with SC-46985 and stimulated with U-46619, an IC₅₀ of 5.6 x 10⁻⁶ M was determined.) c. Inhibition of collagen induced platelet aggregation by SC-44161 and SC-46984 The IC₅₀s for inhibition of collagen induced aggregation by SC-44161 and SC-46984 in dog PRP were 1.14 x 10⁻⁵ M and 2.09 x 10⁻⁶ M, respectively. II. Ex vivo - Inhibition of U-46619 induced aggregation a. Intravenous Figure 1 shows the data obtained when rats were intravenously injected with SC-44161, SC-46984 and SC-46985. In this model, SC-44161 had an ED₅₀ of 31 µg/kg. The two enantiomers of SC-44161, (SC-46984 and SC-46985) were tested in the same model for comparison. SC-46984 has an ED₅₀ of 8.7 µg/kg (approximately 3.5 times more potent than SC-44161) while SC-46985 had an ED₅₀ of 45 µg/kg (approximately 1.5 times less potent than SC-44161). b. Intragastric The potency of SC-44161 was determined when compound was administered I.G. and compared with SC-46984 and SC-47721 (Figure 2). SC-44161 had an I.G. ED₅₀ of 1.21 mg/kg. In the same model SC-46984 had an I.G. ED₅₀ of 0.44 mg/kg (approximately 2.8 times more potent than SC-44161). For comparison SC-47721 was tested at 1 mg/kg and gave 37.5% inhibition at that dose. c. I.G./I.V. ratio From the I.V. and the I.G. studies, an I.V./I.G. ratio was calculated. For SC-44161 the I.V./I.G. ratio was 39 and for SC-46984 the ratio was 51. This difference in I.V./I.G. ratios is probably not large enough to be important. INHIBITION OF U-46619 INDUCED AGGREGATION IN VITRO IN RAT PLATELET RICH PLASMA COMPOUND IC₅₀ (M) EtOH Na₂CO₃ SC-44161 2.6 x 10⁻⁷ 7.0 x 10⁻⁸ SC-46984 5.0 x 10⁻⁸ 7.0 x 10⁻¹⁰ SC-46985 9.7 x 10⁻⁷ ∼2.5 x 10⁻⁶ SC-46986 6.9 x 10⁻⁷ N.D. SC-47721 N.D. ∼7.5 x 10⁻⁹ N.D. = not done

Claims: A compound being 7-[3α-[1-[[(phenylamino) thioxomethyl]hydrazono]ethyl]-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid and having the structure and pharmaceutically acceptable salts thereof. A compound being 7-[3α-[1-[[(phenylamino) thioxomethyl]hydrazono]ethyl]-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid and having the structure and pharmaceutically acceptable salts thereof. A compound being 7-[3α-acetyl-1R,1α,4α-bicyclo[2.2.1]-hept-2β-yl]-5Z-heptenoic acid and having the formula A compound being 7-[3α-acetyl-1R,1α,4α-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid and having the formula A method of making a compound of the formula (I) or a pharmaceutically acceptable salt thereof, the compound or salt being the cis(Z) isomer of Claim 1 or the trans(E) isomer of Claim 2, which comprises the steps of (a) resolving a compound of the formula into the respective diasteneomers of the formulae by reacting the compound with a diol whose hydroxyl functions are attached to adjacent carbon atoms, (b) hydrolyzing the optically active product of (a); (c) oxidizing the product of (b) by peracid oxidation; (d) reducing the product of (c); (e) reacting the product of (d) with (4-carboxybutyl)triphenyl-phosphonium bromide followed by oxidation to produce a ketone which is isomerized with base to the stable isomer; and (f) reacting the ketone product of (e) with 4-phenyl-3-thiosemicarbazide; and where appropriate (g) forming a pharmaceutically acceptable salt of the product of (f). A method according to Claim 5, wherein the hydroxyl functions of the diol are attached to adjacent chiral atoms having symmetry elements defining C₂ symmetry. A method according to Claim 6, wherein the diol is (2R,3R)-2,3-butanediol or (2S,3S)-2,3-butanediol. A method of making a compound of the formula (I) the compound or salt being the cis(Z) isomer of Claim 1 or the trans(E) isomer of Claim 2, or a pharmaceutically acceptable salt thereof, which comprises the steps of (a) reacting a compound of the formula with a triflating agent in the presence of a base to form a compound of the formula which is then methylated to form a compound of the formula (b) hydroborating and oxidizing the product of (a); (c) oxidizing the product of (b) by peracid oxidation; (d) reducing the product of (c); (e) reacting the product of (d) with (4-carboxybutyl)triphenylphosphonium bromide followed by oxidation; and (f) reacting the product of (e) with 4-phenyl-3-thiosemicarbazide; and where appropriate (g) forming a pharmaceutically acceptable salt of the product of (f). A method according to any of Claims 5 to 8, wherein the carbon-carbon double bond in the compound of formula (I) has the cis(Z) configuration. A pharmaceutical composition comprising a compound according to Claim 1 or 2 or a pharmaceutically acceptable salt thereof together with a pharmaceutically acceptable diluent or carrier. A compound according to Claim 1 or 2 or a pharmaceutically acceptable salt thereof for use in therapy or diagnosis.

Inventors: GARLAND, ROBERT BRUCE, MIYANO, MASATERU
Applicants: BRITISH TECHNOLOGY GROUP LIMITED
Application year: 1989
Main classification: C07C405
IPC codes: A61K31, C07C405, C07C1, A61P7, C07C13, C07C337, C07D311, C07C49, C07C59