Anti-microbial composition

Abstract: Medical instruments, which may include brass, copper, aluminum, stainless steel, carbon steel, or plastic parts are sterilized or disinfected in an anti-microbial solution. The anti-microbial effect of a strong oxidizing agent, such as peracetic acid, is improved with a wetting agent. The solution further includes a triazole or other component for inhibiting the corrosion of copper and brass. Phosphates or other buffering agents adjust the oxidizing agent generally to a neutral pH for preventing the corrosion of steel. Molybdates or analogous compounds also buffer the pH and inhibit corrosion of aluminum by the oxidizing agent. The corrosion inhibiting is enhanced by the wetting agent. Optionally, a sequestering agent is provided for inhibiting hard water precipitation.

Description: The present invention relates to antimicrobial compositions and methods of using them. It finds particular application in conjunction with automated sterilizing or disinfecting of medical instruments and will be described with particular reference thereto. However, it is to be appreciated that the present invention will find utility in sterilizing and disinfecting a wide range of objects, either automatically or manually. Heretofore, most medical instruments have been sterilized in a steam autoclave. In hospitals and large facilities, medical instruments and equipment were transported to a central sterilizing facility where they were sterilized under the supervision of sterilizing room technicians. In a steam autoclave, the equipment was subject to superheated steam at high pressures, depressurized, and cooled. One of the drawbacks of the steam autoclave is that many medical instruments cannot withstand the high temperatures and pressures. Another drawback resides in the one to two hour cycle time. Instruments and equipment which could not withstand the pressure or temperature of the autoclave were commonly sterilized with ethylene oxide gas. The equipment was sealed in a sterilizing chamber which was pressurized with the ethylene oxide gas. After an appropriate sterilizing cycle, the equipment was degassed for twelve to sixteen hours in a vacuum or about 72 hours in ambient atmospheric conditions to remove the highly toxic ethylene oxide. One of the drawbacks to ethylene oxide sterilization resided in the long cycle times. Another drawback resided in the need for training technicians to handle the highly toxic ethylene oxide gas systems. Yet another drawback was that some medical equipment could not be sterilized with ethylene oxide gas. Liquid sterilization systems were utilized for equipment which could not withstand either the autoclave or the ethylene oxide. The equipment was immersed in a vat or tank that had been filled with a sterilizing solution, such as stabilized hydrogen peroxide or glutaraldehyde. Because such liquid sterilizations were normally performed manually, the skill and care of the technician were controlling factors in whether sterilization or disinfection were, in fact, attained. In many instances, the technician was required to mix the components of the anti-microbial composition. Even when mixed properly, relatively long immersion times on the order of six to ten hours were commonly required to assure sterilization. Moreover, many liquid sterilization systems were highly corrosive to metal parts, particularly brass, copper, and aluminum. With long immersion times, even carbon steel and stainless steel could be pitted and sharp cutting edges dulled. Chemical Abstracts, vol. 93, no. 6, 11th August 1980, page 353, abstract no. 52264d discloses a means of protecting from corrosion iron, bronze, and brass in peracetic acid solutions by the use of Na 2 H 2 P 2 O 7 or a 1:1 mixture of that compound with NaH 2 PO 4 H 2 O. Chemical Abstracts, vol. 83, no. 8, 25 August 1975, page 385, abstract no. 64532w discloses a means of reducing the corrosive effect of peracetic acid solutions on copper, brass and bronze by the addition of NaH 2 PO 4 . Derwent Japanese Patents Gazette, section Ch, week Y46, 3rd January 1978 describes a means of preventing corrosion by ozone solution in metal water pipes, using sodium hexametaphosphate and potassium bichromate. US-A-4 518 585 (D.F. Greene et al) discloses aqueous acidic disinfecting and sterilizing compositions containing hydrogen peroxide, a surfactant and a triazole corrosion inhibitor. In accordance with the present invention, a new and improved anti-microbial composition is provided which overcomes the above-mentioned problems. In a first aspect the invention provides an anti-microbial composition characterised in that it comprises: (a) a strong oxidant selected from peracetic acid and lithium hypochlorite; (b) a copper and brass corrosion inhibitor selected from five-membered ring compounds and benzoates; (c) a buffering agent which buffers the composition to a pH in the range from pH 6.4 to pH 7.9; (d) an anti-corrosive agent which inhibits corrosion of carbon steel, stainless steel and aluminum; (e) a wetting agent. In a second aspect the present invention provides an anti-microbial composition, characterised in that it comprises: (i) a strong oxidant selected from peracetic acid and lithium hypochlorite; (ii) an azole as an inhibitor of copper and brass corrosion; and (iii)a phosphate as a buffering agent which buffers the composition to a pH in the range from pH 6.4 to pH 7.9, and as an anti-corrosive agent. A third aspect of the present invention provides a method of sterilising or disinfecting, characterised in that a surface to be sterilised or disinfected is brought into contact with an anti-microbial composition according to said first or second aspect of the invention. Where the strong oxidant is lithium hypochlorite the composition is conveniently buffered to a pH in the range 6.95 to 7.9, for example in the range 6.95 to 7.0. Where the strong oxidant is peracetic acid the composition is conveniently buffered to a pH of 6.4. The anti-microbial composition, whether according to the first or second aspect of the invention, is conveniently in the form of an aqueous solution. In accordance with another aspect of the present invention, an anti-microbial solution is provided which comprises peracetic acid or lithium hypochlorite, a copper and brass corrosion inhibitor, an aluminum corrosion inhibitor, a steel corrosion inhibitor and buffering agent, and a wetting agent. If tap water is used as a dilutant it may be convenient - though not essential - to use a sequestering agent. Where the strong oxidant is peracetic acid it is preferably in an amount in the range 0.005 - 1.0%, for example 0.2%. Where the strong oxidant is lithium hypochlorite it is conveniently used in an amount of 0.05 to 0.13%. In accordance with another more limited aspect of the invention, the copper and brass corrosion inhibitor can comprise a triazole or other azole. Where it is a triazole it can be particularly benzotriazole or tolyltriazole. In accordance with another more limited aspect of the invention, the aluminum and steel corrosion inhibitor and the buffering agent are selected from the class consisting of chromates, dichromates, borates, phosphates, molybdates, vanadates, and tungstates. More specifically to a preferred embodiment, phosphates are preferred for inhibiting steel corrosion and buffering the solution to a substantial pH. Molybdates are preferred for inhibiting aluminum corrosion. As explained below, the use of a wetting agent is advantageous. However, excellent results can be obtained using compositions of the invention without a wetting agent. This is clearly illustrated by Example 1 below where excellent results were obtained on a range of metals using a peracetic acid composition containing no wetting agent. In accordance with a more limited aspect of the invention, a method of sterilizing medical instruments is provided. Powdered water soluble reagents including a copper and brass corrosion inhibitor, an anti-corrosive buffering agent, a sequestering agent, and a wetting agent are dissolved in water and circulated through the plumbing of an automated sterilizing apparatus and over the medical instruments to be sterilized. A concentrated, peracetic acid or lithium hypochlorite agent is diluted into the water with dissolved powder reagents and circulated through the plumbing of the automated apparatus and over the medical instruments. After the diluted antimicrobial has remained in contact with the medical instrument for a selected duration, the anti-microbial solution is drained and the medical instrument is rinsed with sterilized water. In accordance with a more limited aspect of the sterilizing method, the reagents are selected from the classes discussed above. One advantage of the present invention is that it provides an antimicrobial agent which quickly sterilizes or disinfects medical equipment or the like. Another advantage of the present invention is that it works on substantially all materials with minimal corrosion. Yet another advantage of the present invention is that it facilitates automated sterilizing and minimizes operator error. Still further advantages of the present invention will become apparent upon reading and understanding the following detailed description. The invention may utilize various components and arrangements of components or in various steps and arrangements of steps. The drawings are only for purposes of illustrating a preferred embodiment and are not to be construed as limiting the invention. FIGURE 1 is a perspective view of a sterilizing apparatus in accordance with the present invention; and, FIGURE 2 is a tubing diagram of the sterilizer of FIGURE 1. With reference to FIGURES 1 and 2, a dilutant or water source 10 supplies water or other fluid reagents. In the preferred sterilizer embodiment, the water source includes a length of tubing 12 connected with a water spigot or other building plumbing and a control valve 14 for selectively preventing and permitting the flow of water to a sterilizing means 16 . In the preferred embodiment, the sterilizing means is a filter which removes particles which are as large or larger than bacteria. Optionally, an inline water treatment means may be provided for modifying the chemical composition of the water. For example, a water softening cartridge may be provided for reducing or eliminating calcium and magnesium salts from the water. Alternately, various water treatments may be added to the water, such as a wetting agent, a sequestering agent, or others of the reagents to be discussed herein below. A tubing system 18 connects the filter or sterilizing means with a container or module 20 for receiving an item to be sterilized. In the preferred embodiment, the container is defined by a removable tray 22 configured in accordance with the item, e.g. an endoscope. A lid 24 is sealed to the tray in a lowered position by a resilient gasket 26 to complete the container. Optionally, a transparent window 28 is defined in the lid. The tubing system includes a spray nozzle 30 and a container distribution manifold 32 for distributing water and other fluids around the item to be sterilized. A reagent receiving well 34 collects the fluid for return. Vent lines 36 enable air to be vented from the container such that it is completely filled with the water or other sterilant solution. Any excess fluid is discharged through check valve 38 into a drain line 40 . A reagent introduction system 42 introduces corrosion inhibitors and peracetic acid or lithium hypochlorite anti-microbial agent into the water. Preferably, the corrosion inhibitors are introduced first and circulated over the item to be sterilized or disinfected before the anti-microbial agent. This provides corrosion protection before the corrosive anti-microbial agent contacts the item. Alternately, the corrosion inhibitors and anti-microbial agent may be introduced concurrently such that both reach the item contemporaneously. An aspirator 44 which draws a oxidizing sterilant concentrate or other liquids from an ampule 46 . In a preferred embodiment, the oxidant is concentrated peracetic acid which has a stable shelf life of several months. More specific to the preferred embodiment, the operator opens a container having a premeasured dose of powdered reagents and the liquid filled ampule. The powdered reagents are emptied into the well 34 and the ampules positioned to be pierced and drained by the aspirator. As described in greater detail below, the powdered reagents include a buffer, e.g. a phosphate, for bringing the pH to a neutral level and to inhibit steel corrosion. An aluminum corrosion inhibitor, such as a molybdate inhibits aluminum and steel corrosion. A copper and brass corrosion inhibitor, preferably a triazole, protects copper and brass components. Moreover, there is a synergistic interaction in which the phosphates, molybdates, triazoles, and wetting agent interact to protect steel, aluminum, copper, and brass better than if any one were eliminated. If tap water, rather than deionized water is used as the dilutant, a sequestering agent, e.g. sodium hexametaphosphate, is included to prevent the precipitation of its calcium and magnesium salts. After placement of the item the operator closes the lid and the system is filled with water. A pump 50 selectively draws water from the container 20 through well 34 and aspirator 44 . Recirculating the water dissolves the powdered reagents and aspirates the sterilant from the ampule and circulates the sterilant and reagents through the tubing system 18 . Preferably, the vent line 36 is very short and of a substantial diameter such that the solution is circulated over exposed surfaces of the drain check valve 38 and a vent check valve 52 . A heating coil adjusts the temperature of the solution. Recirculation continues until the interior of the container and all exposed surfaces of the tubing system and valves are sterilized. Alternately, once fully dissolved, the sterilant may remain quiescent for a selected duration. After the preselected sterilization or disinfecting period, the sterilant solution is drained through a drain valve 56 and sterile air is drawn into the system through an air sterilizing means - preferably a filter that removes any particles the size of a bacteria or larger. The filled valve 14 is opened and the drain valve 56 is closed such that the sterile filter 16 provides a source of sterile rinse. Note that the sterile rinse passes only along sterilized surfaces of the tubing system and valves in order to assure sterility. Every tubing and valve surface from the filter to the drain was exposed to the circulating sterilant solution for a sufficient duration to assure its sterility. The pump 50 circulates the sterile rinse through the system for a selected duration sufficient to rinse residue that the buffered strong oxidant tends to deposit. At the end of the rinse cycle, the rinse solution is drained by opening drain valve 56 . When a return valve 60 is closed, the pump 50 functions to pump liquid from the system out the drain 40 . Additional drain lines (not shown) and aspirators or pumps (not shown) may be provided for removing liquids from every region of the system. The exact location of such additional drains will be dependent on the bends and contours of the plumbing system. In this preferred embodiment, the ampule contains 35% peracetic acid and appropriate stabilizers, as are known in the art to provide an acceptable shelf life. The volume of the ampule relative to the volume of the tubing system 18 is selected such that the peracetic acid is diluted to about 0.2% by weight per volume solution. However, final solutions of .005 - 1% may prove acceptable. The anti-microbial agent and its concentration, and the contact time with the item will vary depending on whether disinfection or sterilization is sought. In many applications, only disinfection is sought. The contact time or the strength composition of the anti-microbial agent may be adjusted accordingly. The copper and brass corrosion inhibitors are preferably benzotriazoles and tolyltriazoles, which are preferred due to their stability in the presence of strong oxidizing compounds. Mercaptobenzathiozol might also be utilized but is more apt to be oxidized or destabilized by strong oxidizers. Azoles, benzoates, and other five membered ring compounds may also prove acceptable as copper and brass corrosion inhibitors. The anti-corrosive buffering compounds are preferably a mixture of phosphate in sufficient volume to produce a final concentration of 1.25% weight per volume and molybdates in an appropriate amount to produce a final solution of .011% weight per volume. Phosphates may also be effective in the range of 0.2% to 12.5% and the molybdates may be effective from 0.1 to 10%. Optionally, borates, chromates, dichromates, tungstates, vanadates and combinations thereof, may be substituted, in appropriate concentrations to inhibit steel corrosion, i.e. buffer to a generally neutral pH, and aluminum corrosion. In hard water, the phosphates tend to cause calcium and magnesium salts to precipitate and coat the instruments being sterilized and parts of the sterilizing system. A sequestering agent appropriate to prevent precipitation, such as sodium hexametaphosphate, may be provided. Of course, if deionized or soft water is utilized the sequestering agent may be eliminated. However, to insure universal applicability with any water that might be utilized, the presence of a sequestering agent is preferred. A wetting agent present to about 0.001 percent (W/V) improves the wetting of the surface of the instrument by the anti-microbial agent. The wetting agent has also been found to increase penetration by the anti-microbial agent improving anti-microbial efficacy while reducing corrosion. The following are examples that illustrate the corrosion inhibiting effectiveness of a peracetic acid anti-microbial composition of the invention (Example 1) compared with peracetic acid alone (Example 2), and of several lithium hypochlorite anti-microbial compositions (Examples 3 to 7) of the invention compared with lithium hypochlorite alone (Examples 8 and 9). Particular attention was paid to corrosion of the metals: Metals A Brass B Stainless Steel C Aluminum D Carbon Steel E Copper In the following Examples, the degree of corrosion has been assessed on the following scale: 1. No corrosion; 2. Slight corrosion; 3. Moderate corrosion; 4. High corrosion; and 5. Heavy corrosion. Example 1 Formula 2000 ppm Peracetic Acid 0.125% Monosodium Phosphate 1.12% Disodium Phosphate 0.08% 1-H-Benzotriazole 0.025% Sodium Hexametaphosphate 0.05% Sodium Molybdate pH 6.4 Exposure Time Metal A B C D E 30 minutes 1 1 1 1 1 16 hours 1 1 1 1 1 24 hours 1 1 1 1 Example 2 Formula 2000 ppm Peracetic Acid pH 2.0 Exposure Time A B C D E 1 Hour 5 3 5 5 Example 3 1000 ppm Lithium Hypochlorite 0.125% Monosodium Phosphate 0.8% 1-H-Benzotriazole 0.025% Sodium Hexametaphosphate pH 7.9 Exposure Time A B C D E 30 minutes 1 1 1 1 90 minutes 1 1 1 1 65 hours 2 1 2 1 Example 4 1300 ppm Lithium Hypochlorite 0.6% Disodium Phosphate 0.52% Monosodium Phosphate 0.09% Sodium Molybdate 0.07% 1-H-Benzotriazole 0.045% Sodium Hexametaphosphate 0.009% C0-720* pH 7.0 Exposure Time A B C D E 30 minutes 1 1 1 1 1 hour 1 1 1 1 Example 5 500 ppm Lithium Hypochlorite 1.25% Monosodium Phosphate 0.5% Sodium Molybdate 0.03% Sodium Hexametaphosphate 0.05% CO-720* 0.08% 1-H-Benzotriazole pH 6.95 Exposure Time A B C D E 30 minutes 1 1 1 1 Example 6 500 ppm Lithium Hypochlorite 1.25% Monosodium Phosphate 1.25% Disodium Phosphate 0.5% Sodium Molybdate 0.05% Sodium Hexametaphosphate 0.08% 1-H Benzotriazole 0.3% 2A1** pH 6.95 Exposure Time A B C D E 30 minutes 1 1 1 1 Example 7 1000 ppm Lithium Hypochlorite 1.25% Monosodium Phosphate 1.25% Disodium Phosphate 1.0% Sodium Molybdate 0.08% 1-H-Benzotriazole pH 7.0 Exposure Time A B C D E 30 minutes 1 1 1 1 24 hours 1 1 1 1 Example 8 1000 ppm Lithium Hypochlorite pH 11.2 Exposure Time A B C D E 1 hour 3 3 5 5 Example 9 500 ppm Lithium Hypochlorite pH 11.0 Exposure Time A B C D E 1 hour 3 3 5 5 Wetting Agents * "CO-720": stands for a wetting agent sold by Rhone-Poulenc under the trade mark "Igepal CO-720". Igepal CO-720 = Poly(oxy-1,2-ethanediyl),alpha-(nonyphenyl)-omega-hydroxy-(C 2 H 4 O) n C 15 H 24 O n = 12 ** A1 = Dodecyl (Sulphenoxy)benzene sulfonic acid, disodium salt and oxybis (dodecyl benzene sulfonic acid), disodium salt

Claims: Claims for the following Contracting States : AT, BE, CH, DE, FR, GB, GR, IT, IL, LU, NL, SE An anti-microbial composition characterised in that it comprises: (a) a strong oxidant selected from peracetic acid and lithium hypochlorite; (b) a copper and brass corrosion inhibitor selected from five-membered ring compounds and benzoates; (c) a buffering agent which buffers the composition to a pH in the range from pH 6.4 to pH 7.9; (d) an anti-corrosive agent which inhibits corrosion of carbon steel, stainless steel and aluminum; (e) a wetting agent. An anti- microbial composition according to Claim 1, wherein said copper and brass corrosion inhibitor comprises a triazole or other azole. An anti-microbial composition according to Claim 2, wherein said copper and brass corrosion inhibitor is selected from benzotriazoles and tolyltriazoles. An anti-microbial composition according to any of the preceding claims, wherein the buffering agent is selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates. An anti-microbial composition according to Claim 4, wherein the buffering agent is a phosphate. An anti-microbial composition according to any of the preceding claims, wherein said anti-corrosive agent is selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates. An anti-microbial composition according to Claim 6, wherein said anti-corrosive agent is a phosphate. An anti-microbial composition according to Claim 5, wherein said phosphate constitutes said anti-corrosive agent and the buffering agent. An anti-microbial composition according to any of the preceding claims, wherein said anti-corrosive agent includes a phosphate and at least one compound selected from molybdates, chromates, tungstates, borates and vanadates. An anti-microbial composition according to any of the preceding claims, further including a sequestering agent to prevent any phosphate present in the composition from causing precipitation in hard water. An anti-microbial composition according to any of the preceding claims, wherein said copper and brass corrosion inhibitor includes a triazole, and said anti-corrosive agent includes at least two compounds selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates. An anti-microbial composition characterised in that it comprises: (i) a strong oxidant selected from peracetic acid and lithium hypochlorite; (ii) an azole as an inhibitor of copper and brass corrosion; and (iii)a phosphate as a buffering agent which buffers the composition to a pH in the range from pH 6.4 to pH 7.9, and as an anti-corrosive agent. An antimicrobial composition according to Claim 1 or Claim 12, wherein the strong oxidant is peracetic acid and the composition is buffered to a pH of 6.4. An anti-microbial composition according to Claim 1 or Claim 12, wherein the strong oxidant is lithium hypochlorite and the composition is buffered to a pH in the range 6.95 to 7.9. An anti-microbial composition according to Claim 14, wherein the strong oxidant is lithium hypochlorite and the composition is buffered to a pH in the range 6.95 to 7.0. An anti-microbial composition, according to Claim 12, which includes a wetting agent. An anti-microbial composition according to any of Claims 12 to 16, wherein the azole is a benzo- or tolyl-triazole. An anti-microbial composition according to Claim 17, wherein the azole is 1-H-benzotriazole. An anti-microbial composition according to Claim 12, which also contains a sequestering agent to prevent phosphate present in the composition from causing precipitation in hard water. An anti-microbial composition according to Claim 1, the composition comprising: 0.005-1% peracetic acid; 0.001-1% triazole; 0.2 - 12.5% phosphate; 0.01 to 10% of a compound selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates; 0 - 0.01% of a sequestering agent; and a wetting agent. An anti-microbial composition according to any of the preceding claims, which is in the form of an aqueous solution. A method of sterilising or disinfecting, characterised in that a surface to be sterilised or disinfected is brought into contact with an anti-microbial composition as claimed in any of Claims 1 to 21. Claims for the following Contracting State : ES A process for the production of an anti-microbial composition, characterised in that it comprises mixing together: (a) a strong oxidant selected from peracetic acid and lithium hypochlorite; (b) a copper and brass corrosion inhibitor selected from five-membered ring compounds and benzoates; (c) a buffering agent which buffers the composition to a pH in the range from pH 6.4 to pH 7.9; (d) an anti-corrosive agent which inhibits corrosion of carbon steel, stainless steel and aluminum; (e) a wetting agent. A process according to Claim 1, wherein said copper and brass corrosion inhibitor comprises a triazole or other azole. A process according to Claim 2, wherein said copper and brass corrosion inhibitor is selected from benzotriazoles and tolyltriazoles. A process according to any of the preceding claims, wherein the buffering agent is selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates. A process according to Claim 4 wherein the buffering agent is a phosphate. A process according to any of the preceding claims, wherein said anti-corrosive agent is selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates. A process according to Claim 6, wherein said anti-corrosive agent is a phosphate. A process according to Claim 5, wherein said phosphate constitutes said anti-corrosive agent and the buffering agent. A process according to any of the preceding claims, wherein said anti-corrosive agent includes a phosphate and at least one compound selected from molybdates, chromates, tungstates, borates and vanadates. A process according to any of the preceding claims, further including a sequestering agent to prevent any phosphate present in the composition from causing precipitation in hard water. A process according to any of the preceding claims, wherein said copper and brass corrosion inhibitor includes a triazole, and said anti-corrosive agent includes at least two compounds selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates. A process for the production of an anti-microbial composition, characterised in that it comprises mixing together: (i) a strong oxidant selected from peracetic acid and lithium hypochlorite; (ii) an azole as an inhibitor of copper and brass corrosion; and (iii)a phosphate as a buffering agent which buffers the composition to a pH in the range from pH 6.4 to pH 7.9, and as an anti-corrosive agent. A process according to Claim 1 or Claim 12, wherein the strong oxidant is peracetic acid and the composition is buffered to a pH of 6.4. A process according to Claim 1 or Claim 12, wherein the strong oxidant is lithium hypochlorite and the composition is buffered to a pH in the range 6.95 to 7.9. A process according to Claim 14, wherein the strong oxidant is lithium hypochlorite and the composition is buffered to a pH in the range 6.95 to 7.0. A process according to Claim 12, which includes a wetting agent. A process according to any of Claim 12 to 16, wherein the azole is a benzo- or tolyl-triazole. A process according to Claim 17, wherein the azole is 1-H-benzotriazole. A process according to Claim 12, which also contains a sequestering agent to prevent phosphate present in the composition from causing precipitation in hard water. A process according to Claim 1, the composition comprising: 0.005-10% peracetic acid; 0.001-1% triazole; 0.2 - 12.5% phosphate; 0.01 to 10% of a compound selected from chromates, dichromates, borates, phosphates, molybdates, vanadates and tungstates; 0 - 0.01% of a sequestering agent; and a wetting agent. A process according to any of the preceding claims, in which said composition is in the form of an aqueous solution. A method of sterilising or disinfecting, characterised in that a surface to be sterilised or disinfected is brought into contact with an anti-microbial composition which has been produced by the process as claimed in any of Claims 1 to 21.

Inventors: BADERTSCHER DUNCAN C, KRALOVIC RAYMOND C, BADERTSCHER, DUNCAN C., KRALOVIC, RAYMOND C.
Applicants: STERIS CORP, STERIS CORPORATION
Application year: 1989
Main classification: A01N59
IPC codes: A01N37, A01N59, A01N25, A61L2, A01N31