Enzyme Stabilisation

Abstract: A method of stabilising enzymes or other proteins against denaturation or drying comprises mixing the protein with a cyclic polyol and a cationic polyelectolyte.

Description: This invention relates to stabilisation of enzymes in the dry state. Few enzymes are inherently stable in solution. Many have a tendency to become denatured when held in solution. Various workers have attempted to stabilise enzymes either by adding compounds such as sugars or glycerol to solutions of them or by freeze drying. These methods often cause a loss of activity. Alternative methods of stabilisation have involved drying of enzymes with stabilisers in a presence of a solid support such as cellulose fibre or polyacrylamide. US 4451569 disclosed stabilisation of glutathione peroxidase by freezing the enzyme with one of a number of sugars including arabinose, glucose, xylitol and sorbitol. Freeze drying is expensive to operate on a large scale and often results in denaturation. PCT/GB86/00396 discloses stabilisation of enzymes by use of the disaccharide trehalose. According to the first aspect of the present invention a method of protecting enzymes against loss of activity on drying comprises the steps of: mixing an aqueous solution of the enzyme with a soluble cationic polyelectrolyte containing group selected from: primary, secondary and tertiary amino and quaternary ammonium groups, and a cyclic polyol, and removing water from the solution. Stabilisation in accordance with this invention enhances the activity of freshly dried enzymes. The stability upon storage is also enhanced. Drying of enzymes is important for many applications, for example use in diagnostic or analytical aids such as test strips which may be stored for prolonged periods before use. Transportation of enzymes in solution is inconvenient and expensive. Although freeze drying may be employed, the present invention facilitates use of vacuum drying and air drying without denaturation. Vacuum drying and air drying are milder processes and are much cheaper to operate. The cyclic polyol may incorporate one or more alicyclic rings any may have at least one side chain. Compounds having 5 to 10 hydroxyl groups may be preferred. Non-reducing polyols are preferred. Di- and trisaccharides are particularly efficacious but other cyclic polyols, for example inositol may also be used. The polyol may be chosen to suit both the enzyme and also the polyelectrolyte in question. Lactitol, lactose, maltose and sucrose are especially preferred in conjunction with DEAE-dextran, lactitol having been found to be most suitable or many applications. Sorbitol is suitable for use with cholesterol oxidase, cholesterol esterase and other enzymes. Cellobiose may also be used. The amount of polyol may lie in the preferred range of 1 to 20%, more preferably 2 to 10% most preferably 5 to 10%. The cationic polyelectrolyte is preferably a polymer with cationic groups distributed along the molecular chain. The cationic groups, which are preferably quaternary ammonium derived functions, may be disposed in side groups pendent from the chain or may be incorporated in it. Natural or artificial polymers may be employed. Natural polymers such as polysaccharides are preferred since many artificial polymers contain residual traces of the inorganic polymerisation catalyst. Diethylaminoethyl dextran (DEAE-dextran) and chitosan are preferred although polyethyleneimine is also suitable. Polysaccharides with MW 5000 to 500 000, preferably 5000 to 20 000, more preferably 5000 to 10 000 may be employed. An amount of 0.1 to 10% is preferred, especially 0.5 to 2%. The pH at which enzymes are dried in accordance with this invention may be important to optimise retention of activity both upon drying and after subsequent storage. The optimum pH for a particular enzyme may be determined by simple experimentation. Alcohol oxidase has been formed to retain activity between pH 7 and 8, preferably at pH 7.8. Cholesterol oxidase, dependent on the source, dries best at pH 5 or 9. Uricase may be dried at pH 9. Cholesterol esterase dependent on source may be dried at pH 7 or 9. Drying is preferably performed in the presence of a wetting agent. Temperatures between 4° and 50°, especially 25° to 35° are preferred. According to a second aspect of the present invention there is provided a dried product containing an enzyme, a cyclic polyol and cationic polyelectrolyte containing groups selected from: primary, secondary and tertiary amino and quaternary ammonium groups, the enzyme being stabilised against loss of activity, provided that the enzyme is not rennin. The dried product may be a free running powder or may comprise part of a test strip or other analytical or diagnostic apparatus. The present invention is now described by means of example but not in any limitative sense. Examples 6 to 9 are comparative examples. Experimental Procedures Percentages used in the specification are by weight unless indicated otherwise. All the stabilisation systems utilise buffers to maintain stable pH conditions eg. Buffer solutions containing Na 2 HPO 4 .2H 2 O (10.855g) and NaH 2 PO 4 .2H 2 O (6.084g) were dissolved in 1.0 litre distilled water to give a solution of pH 7.0 at a concentration of 100 millimoles per litre. An alternative buffer is MOPS 3-N-morpholinopropane sulphonic acid) 52.25g/2.51 distilled water pH to 7.87 with 4.0M.NaOH. A wetting agent may be used dependent on whether or not the enzyme system is being stabilised in a polystyrene cuvette. A suitable wetting agent is protein hydrolysate from gelatine termed Byco A. These are made up to 1% w/v in phosphate buffer, 100 mmol.1 -1 , pH 7.0 as needed. Enzyme solutions were made up freshly before use. Stock solutions of enzymes in ammonium sulphate solution were dialysed exhaustively against buffer eg 100 mmol.l -1 phosphate buffer pH 7.0 to remove all salts. Stock enzyme concentration may be from 10 to 1000 units of activity per millilitre of solution. In terms of protein concentration this is between 0.5 to 200 mgcm -3 . Typically the final protein concentration was 1.0 mgcm -3 . Soluble polyelectrolytes, polyols , enzyme, buffer salts and wetting agent (if used) were mixed at constant temperature and dried in a vacuum oven over dessicant eg. silica gel, 0.1mm/Hg, 30°C for 4-10 hr. The oxidase enzymes studied may be assayed by colorimetric detection of the hydrogen peroxide produced by action of the enzyme. Peroxidase acts on the hydrogen peroxide produced in the presence of aromatic alcohols or amines and the heterocyclic compound 4-aminoantipyrine to give quinoneimine dyes. Other standard assays systems may be employed eg u.v, spectrometry. The following systems were employed: System 1 Phenol sulphonic acid 25 mmol.l -1 4-aminoantipyrine 0.4 mmol.l -1 Peroxidase 1000 unit/1 The resultant dye was measured at 500nm. System 2 3,5-dichloro 2-hydroxybenzene sulphonic acid 10 mmol.l -1 4-aminoantipyrine 0.4 mmol.l -1 Peroxidase 1000 units/1 The resultant dye was measured at 520 nm. Standard temperature eg. 25°C and incubation times eg. 5 minutes were used. Reagent blanks contained all components except substrate. Dry preparations in cuvettes were reconstituted with Systems 1. or 2. directly. Dry powdered preparations were reconstituted with phosphate buffer and suitable aliquots were added to System 1. or 2. For stability trials the storage temperature was 37°C, with samples being removed periodically to check for residual activity of the enzyme. This procedure was standard for all enzymes tested. Soluble Polyelectrolyte and Sugar Alcohol or Saccharide Soluble Polyelectrolyte Soluble polyelectrolyte was dissolved in distilled water a concentration up to 20% w/v, usually to 10% w/v. Sugar alcohol or saccharide was dissolved in distilled water up to a concentration of 40% w/v, usually to 20% w/v. These solutions were used within 4 weeks of preparation, being stored in the cold at 4°C. Example 1 Solution 1 DEAE-Dextran 10% 100ul Lactitol 20% 500ul Byco A 1% 100ul Solution 2 Alcohol oxidase 7 units (1.7mg protein 35ul Phosphate buffer 100mmol.l -1 pH 7.0 265ul Solution 1 was stirred continuously whilst slowly adding Solution 2 at 4°C. The mixture was stirred for 5 minutes to ensure complete mixing. 0.1ml volumes were dried in cuvettes as described, stored at 37°C and assayed for activity as described (Table 1). Example 2 Solution 1 Alcohol oxidase 2 411 units (= 422 mg protein) in phosphate buffer 300mmol.l -1 2.7cm 3 Solution 2 Lactitol 20% w/v 3.0cm 3 DEAE-Dextran 10% w/v 0.27cm 3 Solution 2 was added slowly to Solution 1 with stirring. The mixed solutions were pipetted into petri dishes and vacuum dried over silica gel at 30°C for 8 hours whereupon a thin glassy film of dried enzyme and stabilizer was produced. This was removed and ground to a fine powder using a glass pestle and mortar. For stability testing 10mg portions of enzyme powder were weighed into sterile polystyrene tubes and incubated at 37° in a sealed container over silica gel. Samples were removed periodically and reconstituted in distilled water. 60ul of reconstituted enzyme solution was added to each assay cuvette containing peroxidase and colour reagents as described (Table 2). Example 3 Solution 1 DEAE-Dextran 10% w/v 100ul Lactitol 20% w/v 500ul Byco A 1% w/v 100ul Solution 2 Choline oxidase 10 units (0.794 mg protein) in phosphate buffer pH 7.0 100cm 3 300ul Solution 2 was added with stirring to Solution 1 and thoroughly mixed at 4°C. 0.1cm 3 volumes were vacuum dried in cuvettes as described, stored at 37°C and assayed for activity as described (Table 3). Example 4 Glycerol 3 Phosphate Oxidase Solution 1 DEAE-Dextran 10% w/v 100ul Lactitol 20% w/v 500ul Byco A 1% w/v 100ul Solution 2 Glycerol 3 Phosphate Oxidase 10 units (0.526 mg protein) in phosphate buffer pH 7.0 100mmol.l -1 300ul Solution 2 was added with stirring to Solution 1 at 4°C and thoroughly mixed. 0.1cm 3 volumes were vacuum dried in cuvettes as described (Table 4). Example 5 Solution 1 DEAE-Dextran 10% 100ul Lactose 20% 500ul Byco A 1% 100ul Solution 2 Alcohol oxidase 5 units (1.0mg protein in 100mmol.l -1 Phosphate buffer pH 7.0 300ul Solution 1 was stirred continuously whilst slowly adding Solution 2 at 4°C. The mixture being stirred for 5 minutes to ensure complete mixing 0.1cm 3 volumes were dried in cuvettes as described (Table 5). Soluble Polysaccharides Soluble polysaccharides were dissolved in distilled water up to a concentration of 30% w/v usually to a concentration of 10% w/v. These solutions were used within 4 weeks of preparation and stored at 4°C. Example 6 Solution 1 Dextran (molecular wt. 10 000) 10% w/v 100ul Byco A 1% w/v 100ul Distilled water 500ul Solution 2 Alcohol oxidase 7 units (1.32 mg protein) in 100mmol.l -1 Phosphate buffer pH 7.0 300ul Solution 2 was added to Solution 1 with stirring at 4°C and stirring was continued for 5 minutes to ensure complete mixing. 0.1cm 3 volumes were vacuum dried in cuvettes, stored at 37°C and assayed for activity as described. When dextrans of differing molecular weights are used variations in stability were noted (Table 6). Example 7 Solution 1 Dextran molecular wt. 10 000 10% w/v solution 500ul Phosphate buffer 10mmol.l -1 pH 7.0 300ul Solution 2 Galactose oxidase 0.52 units (0.8 mg protein) in 10mmol.l -1 phosphate buffer pH 7.0 200ul Solution 2 was added to Solution 1 with stirring at 4°C and stirring was continued for 5 minutes to ensure complete mixing. 0.1 ml aliquots were vacuum dried, stored at 37°C and assayed for activity as described (Table 7). Cyclic Polyalcohol Cyclic polyalcohol was dissolved in distilled water to a concentration of 10% w/v. The solutions were stored at 4°C and used within 4 weeks of preparation. Example 8 Solution 1 Inositol 10% w/v 500ul Distilled water 200ul Solution 2 Alcohol oxidase 4.7 units 91.15 mg/protein) in Phosphate buffer 100mmol.l -1 200ul Solution 2 was added to Solution 1 with stirring t 4°C and stirring was continued for 5 minutes to ensure complete mixing. 0.1cm 3 aliquots were vacuum dried, stored at 37°C and assayed for activity as described (Table 8). Example 9 Solution 1 Inositol 10% w/v 500ul Phosphate buffer 100mmol.l -1 pH 7.0 300ul Solution 2 Galactose oxidase 0.52 units (0.8 mg protein) in 10mmol.l -1 phosphate buffer pH 7.0 200ul Solution 2 was added to Solution 1 with stirring at 4°C and stirring was continued for 5 minutes to ensure complete mixing. 0.1cm 3 aliquots were vacuum dried as described, stored at 37°C and assayed for activity as described (Table 9). Example 10 The following results show the stabilisation of alcohol oxidase (Hansenula polymorpha). Unstabilised enzyme retained 26% activity after 7 days incubation at 37°C. Addition of chitosan above gave retention of 48.9% activity after 9 days. The activity in relation to freshly dried enzyme was measured after incubation at 37°C. Example 11 The following results show stabilisation of alcohol oxidase (Pichia pastoris). Unstabilised enzyme retained 49.8% and 36.1% activities after 2 days and 13 days respectively at 37°C. Enhanced activity (ie greater than 1-%) upon drying may be attributable to selective degradation of inhibiting impurities. Stabiliser Period of incubation days Activity/% lactitol 5% 1 102.5 DEAE-Dextran 1 % 4 116.6 8 121.3 15 104.3 dextran 5% 1 83.2 4 97.0 8 101.7 15 87.6 inositol 5% 1 88.0 4 106.2 8 107.1 15 109.1 Example 12 The following results illustrate stabilisation of cholesterol oxidase (Nocardia erythropolis). Unstabilised enzyme retained 34.3% activity after 14 days at 37°C. Stabiliser Period of incubation days Activity/% lactitol 5% 3 96.2 DEAE-dextran 1% 5 105.6 14 115.7 inositol 5% 1 92.6 7 84.8 10 91.7 Example 13 The following results illustrate stabilisation of freeze dried uricase. Stabiliser Period of incubation days Activity/% lactitol 5% 1 109.9 DEAE-Dextran 1% 5 114.3 10 109.9 Example 14 The following results illustrate stabilisation of various enzymes with lactitol (15%) and DEAE-dextran (1%) during drying in comparison to the activity of undried enzymes. Enzyme Activity after drying/% Unstabilised Stabilised Alcohol oxidase (Pichia) 64.7 78.2 Choline oxidase 63.3 97.7 Lactate oxidase 77.1 90.0 Alcohol oxidase (Hensenula polymorpha) 68.2 119.6 Cholesterol oxidase (vacuum dried) 80.0 92.5 (inositol 5%) (freeze dried) 79.0 91.0 (inositol 5%) Preparation Incubation 37°C % Activity remaining relative to activity freshly dried enzyme Alcohol oxidase + 1 day 108 Lactitol 10% 7 days 120 DEAE-Dextran 1% 14 days 114 21 days 106 5 months 102 Unstabilised Enzyme retained 26% activity after 7 days Bulk preparation Incubation 37°C % Activity retained relative to freshly dried enzyme DEAE-Dextran 1% 4 days 138 Lactitol 10% Alcohol oxidase 12 days 121 7.25 units/10 mg solid Unstabilised Enzyme retained 34% activity after 4 days. Preparation Incubation 37°C % Activity remaining relative to freshly dried enzyme Choline oxidase 1 day 99 DEAE-Dextran 1% 5 days 84 Lactitol 10% 10 days 81 15 days 83 Unstabilised Enzyme retained 24% of activity after 1 day, decreasing to 11% after 5 days. Preparation Incubation 37°C % Activity remaining relative to freshly dried enzyme Glycerol 3 phosphate oxidase 1 days 104 5 days 120 DEAE-Dextran 1% 10 days 117 Lactitol 10% 15 days 113 Unstabilised enzyme retained 94% activity after 1 day but only retained 54% activity after 15 days. Preparation Incubation 37°C % Activity remaining relative to freshly dried enzyme Alcohol oxidase 1 day 137 DEAE-Dextran 1% wt/vol 6 days 103 Lactose 10% wt/vol 10 days 108 15 days 92 Unstabilised enzyme retained 23% of activity after 10 days at 37°C. Preparation % Activity remaining (relative to freshly dried enzyme) Alcohol oxidase + Dextran 1% wt/vol 1 day 37°C 6 days 37°C 18 days 37°C 11 months 37°C M Wt. T10 10 000 93 87 73 77 T40 40 000 82 75 61 64 T70 70 000 84 86 65 60 T500 500 000 83 86 62 49 T2000 2 000 000 45 43 22 14 Unstabilised enzyme retained 30% of activity after 6 days. Galactose oxidase Dextran concentrations 5% (M.W 10 000). Incubation 37°C % Activity remaining relative to activity of freshly dried enzyme. 1 day 92% 7 days 87% 10 days 82% Preparation Incubation 37°C % Activity remaining relative to freshly dried enzyme Alcohol oxidase + 1 Day 150% 5% Inositol 7 days 196% 14 days 166% 23 days 178% Unstabilised enzyme retained 26% activity after 7 days. Preparation Incubation 37°C % Activity remaining relative to freshly dried enzyme Galactose oxidase + 1 day 85% 5% Inositol 4 days 78% 10 days 74%

Claims: A method of protecting enzymes against loss of activity on drying comprising the steps of: mixing an aqueous solution of the enzyme with a soluble cationic polyelectrolyte containing groups selected from: primary, secondary and tertiary amino and quaternary ammonium groups and a cyclic polyol, and removing water from the solution. A method as claimed in Claim 1, wherein said polyelectrolyte comprises a quaternary ammonium functionalised polysaccharide. A method as claimed in claim 2, wherein said polyelectrolyte comprises diethylaminoethyl-dextran or chitosan. A method as claimed in Claim 1, wherein the polyelectrolyte comprises polyethyleneimine. A method as claimed in any preceding claim, wherein the polyol is a di or trisaccharide. A method as claimed in Claim 5 wherein the polyol is selected from the group comprising: lactitol, lactose, maltose, sucrose and cellobiose. A method as claimed in any preceding claim, wherein water is removed at a temperature between 4° and 50°C. A method as claimed in Claim 7, wherein the temperature is 25° to 35°C. A dried product containing an enzyme, a soluble cationic polyelectrolyte containing groups selected from: primary, secondary and tertiary amino and quaternary ammonium groups and a cyclic polyol, the enzyme being stabilised against loss of activity in accordance with the method of any preceding claim, provided that the enzyme is not rennin.

Inventors: GIBSON TIMOTHY DAVID, WOODWARD JOHN ROBERT, GIBSON, TIMOTHY DAVID, WOODWARD, JOHN ROBERT
Applicants: CRANFIELD BIOTECH LTD, CRANFIELD BIOTECHNOLOGY LTD
Application year: 1989
Main classification: C12N9
IPC codes: C12N9, C07K16, C07K1