Pantethine as inhibitory agent of hepatic fibrosis

Abstract: The invention relates to the use of pantethine as inhibitory agent of hepatic fibrosis.

Description: This invention relates to the use of pantethine as an active ingredient for preparing a pharmaceutical composition for treating hepatic fibrosis. Hepatic fibrosis means an abnormal increase in fibrous connective tissue following hepatic diseases such as alcoholic hepatitis. Hepatic fibrosis induces excessive deposition of connective tissue such as collagen and, at the same time, causes dysfunction of the liver. The progressing of hepatic fibrosis finally causes cirrhosis. Intensive studies have conventionally been made on agents for preventing or inhibiting hepatic fibrosis but have not yet found an effective agent. Pantethine (D-bis-(N-pantothenyl-β-aminoethyl)-disulfide) has been reported to be effective on hepatic diseases such as medicinal fatty liver in JP-B-No. 60-19891 (the term "JP-B" as used herein means an "examined published Japanese patent application), JP-A-55-76816 and Med. Pharm. 1979, 13(1), 34-38 and viral hepatitis in JP-A-No. 58-35118 (the term "JP-A" as used herein means an "unexamined published Japanese patent application"). In Clin. Ther. 1984, 110/3, 251-257, a combination of calcium phosphoryl choline chloride and pantethine was used in liver patients. The combination showed a favorable effect on fatty liver. These effects of pantethine, however, have no connection with the inhibition of hepatic fibrosis. As a result of extensive researches, the inventors have found that pantethine inhibited hepatic fibrosis. This invention therefore relates to the use of pantethine as an active ingredient for preparing a pharmaceutical composition for treating hepatic fibrosis. Pantethine is a compound of high safety, and the acute toxicity (LD₅₀) thereof in mice is over 10 g/kg-body weight (p.o.). Dosage forms of the inhibitory agent of hepatic fibrosis according to the present invention include tablets, powders, granules, capsules, injectable solutions. These dosage forms can be prepared using conventional pharmaceutical techniques by combining pantethine, the main ingredient, with vehicles (e.g., starch, cellulose), disintegrators, stabilizers. The agent of the invention is usually administered orally or parenterally at a dose level of from 200 to 2,000 mg/day for an adult (about 60 kg body weight) in oral administration. The main component of fibrous connective tissue playing an important role in hepatic fibrosis is collagen, and it is known that collagen synthesis is accelerated during the progression of hepatic fibrosis. It is also known that prolyl hydroxylase acts as a rate limiting enzyme in collagen synthesis and the increase in the activity of the enzyme in the liver is closely correlated with the appearance and progression of hepatic fibrosis. As it is demonstrated in the example hereinafter given, the agent of the present invention significantly inhibited an increase in prolyl hydroxylase activity in the liver of a hepatic fibrosis model induced by cholesterol-feeding. The agent of the present invention is therefore excellent as an inhibitory agent of hepatic fibrosis. The present invention is now illustrated in greater detail by the following example. EXAMPLE Test Animals : Male Japanese white rabbits weighing 2.5 kg were used. A cholesterol group consisting of 8 rabbits were fed on RC-4 diet (sold by Oriental Kobo Co., Ltd.) containing 0.5% cholesterol for 10 weeks. A pantethine group consisting of 9 rabbits were fed on RC-4 diet containing 0.5% cholesterol and 1% pantethine for 10 weeks. A control group consisting of 3 rabbits were fed on RC-4 diet for the same period. Prolyl Hydroxylase Assay : The rabbits were sacrificed by exsangination from abdominal aorta under anesthesia with pentobarbital and the liver and kidney were removed. A portion of the organ was homogenized with 9 times the volume of a 0.01M Trishydrochloric acid buffer solution (pH 7.4) containing 10⁻⁵M EDTA and 10⁻⁴M DTT in Polytron homogenizer. The prolyl hydroxylase activity was expressed as radioactivity of ³H₂O released per milligram of protein when ³H-proline labeled unhydroxylated collagen was incubated as a substrate for 30 minutes. That is, 800 µℓ of a substrate mixture having the composition shown in Table 1 below was added to 200 µℓ of the homogenate. Incubation was carried out at 30°C for 30 minutes. 100 µℓ of a 55% trichloroacetic acid aqueous solution was added thereto to stop the reaction. The ³H₂O of the reaction mixture was separated by vacuum distillation of the whole reaction mixture. An aliquot (800 µℓ) of the distillate was mixed with 10 mℓ of Aquasol and counted for the radioactivity in a liquid scintillation counter. The protein amount was measured by BIO RAD Protein Assay Kit using bovine serum albumin (BSA) as a standard. Composition of Substrate Mixture 0.5M Tris-HCℓ buffer solution 3.1 mℓ Ascorbic acid (220 mg/250 mℓ) 3.1 mℓ FeNH₄SO₄ (198 mg/250 mℓ) 3.1 mℓ 1% BSA 3.1 mℓ Catalase (1 g/50 mℓ) 3.1 mℓ α-Ketoglutaric acid (36.5 mg/250 mℓ) 3.1 mℓ ³H-Proline-labeled collagen solution 3.1 mℓ Distilled water 2.48 mℓ The prolyl hydroxylase acivity of the liver and the kidney thus determined are shown in Table 2. Prolyl Hydroxylase Activity in Liver and Kidney Group Prolyl Hydroxylase Activity (cpm/mg-protein) Liver Kidney Normal group 522.8 ± 45.8 Cholesterol group 1349.6 ± 145.0 600.9 ± 79.5 Pantethine group 729.3 ± 45.6 672.3 ± 65.8 As is apparent from Table 2, the prolyl hydroxylase activity in the liver of the cholesterol group significantly increased to about 2.6 times the level of the normal group. This stimulation of the activity of prolyl hydroxylase indicates that cholesterol-feeding induced an increase in collagen synthesis, and hepatic fibrosis was observed in the cholesterol group. To the contrary, the prolyl hydroxylase activity in the liver of the pantethine group was decreased nearly to the level of the normal group by administering pantethine. Pantethine was thus proved inhibitory on hepatic fibrosis. On the other hand, pantethine exerted no effect on prolyl hydroxylase in the kidney, suggesting that the inhibitory activity of pantethine on fibrosis is specific to hepatic fibrosis.

Claims: The use of panthetine as an active ingredient for preparing a pharmaceutical composition for treating hepatic fibrosis.

Inventors: TOMIKAWA MUNEHIRO, WAKASUGI JUNICHIRO, TOMIKAWA, MUNEHIRO, WAKASUGI, JUNICHIRO
Applicants: DAIICHI SEIYAKU CO, DAIICHI PHARMACEUTICAL CO., LTD.
Application year: 1989
Main classification: A61K31
IPC codes: A61K31