Device and method for assay of liquid sample

Abstract: A device for colorimetric assay of at least one component in a liquid sample, which device comprises a sup­ port having a through hole with an area of 3 to 80 mm², a transparent porous film which is provided on the support to cover the hole, a reagent layer provided on a surface of the porous film which is not contacted to the support, and a sample-holding layer which covers the reagent layer and a part of a surface of the support, with which an amount or concentration of said at least one component in the liquid sample is easily and accurately measured.

Description: The present invention relates to a device and a method for quick and easy assay of a liquid sample, particularly body liquids such as whole blood, blood serum, blood plasma, urea and a cerebrospinal liquid. For the assay of a particular component in the liquid sample, an enzymatic analysis is widely used since it can be carried out under mild conditions. In clinical tests, the body liquid is analyzed with a reagent solution containing an oxidase which oxidizes a particular component, a pigment precursor and optionally a peroxidase. In such enzymatic analysis, hydrogen peroxide, which is generated through oxidation of the particular component with the oxidase, directly oxidizes the oxidizable pigment precursor or proceeds oxidation coupling with the pigment precursor in the presence of the peroxidase to generate a pigment. Then, the generated pigment is colorimetrically measured by, for example, absorptiometry, fluorophotometry and emission spectroscopy, and in turn, the amount or concentration of particular component is indirectly measured. Examples of the oxidases useful in the clinical tests are glucose oxidase, uricase, cholesterol oxidase, glycerol-triphosphate oxidase, cholin oxidase, acyl-CoA oxidase, sarcosine oxidase, various amino acid oxidases, bilirubin oxidase, lactate oxidases, lactose oxidase, pyruvate oxidase, galactose oxidase, glycerol oxidase and the like. As the pigment precursor, oxidizable pigment precursors such as a so-called Trinder's reagent (cf. Ann. Clin. Biochem., 6 , 24 (1960)), o-anisidine, benzidine, o-tolidine and tetramethylbenzidine are well known. One of advantages of the enzymatic analysis resides in that, if the component to be analyzed is changed, the same color developing system can be used by changing the kind of oxidase. Therefore, the application of the enzymatic analysis to various analytical items is investigated. Recently, to quickly and conveniently carry out the assay, a reagent system fixed on a solid support is advantageously used in place of the conventional reagent solution. For example, U.S. Patent No. 3,630,957 and Japanese Patent Publication No. 33800/1974 discloses a water-resistant test film which comprises a plastic film and a polymer layer in which an assay system comprising the above oxidase and hydrogen peroxide is dispersed. In the assay with such water-resistant test film, since the color of the generated pigment is measured from the side on which the sample such as the whole blood or the blood serum is applied, the sample should be wiped out with a piece of absorbent cotton and the like after contacting the sample to the reagent layer for a certain period of time. Further, since the color is developed in the presence of a sufficient amount of oxygen after wiping out of the sample, the test film should be kept standing in the air for a certain period of time. U.S. Patent No. 3,992,158 and Japanese Patent Publication No. 21677/1978 discloses a multi-layer test film comprising a liquid-impermeable transparent support, a reagent layer and a spreading layer. When the liquid sample is applied at a point of the spreading layer, it spreads over the spreading layer and then migrates into the reagent layer. The color of the pigment which is generated through the reaction between the specific component and the reagent is measured through the transparent support. Therefore, it is not necessary to remove the applied sample. However, since the reagent layer is present between the support and the spreading layer, it is difficult for the air to reach the reagent layer through the spreading layer. Particularly when the reagent layer contains the oxidase, the reaction does not proceed sufficiently due to shortage of oxygen. To overcome such drawback, EP-A-137 521 and Japanese Patent Kokai Publication No. 82859/1985 discloses a multi-layer integral element for chemical analysis, which comprises an oxygen-permeable protein-impermeable light shielding layer between the reagent layer and the spreading layer to improve the contact between the reagent layer and the air. However, since the reagent layer never contacts the air on the support side, the oxygen is not sufficiently supplied to the reagent layer. German Patent No. 3 510 992 and Japanese Patent Kokai Publication No. 205364/1985 discloses an assay device having a porous hydrophobic oxygen-supplying layer between the reagent layer and the support. Then, such device can significantly increase the amount of the oxygen supplied to the reagent layer containing the oxidase. But, since the oxygen-supplying layer has a certain thickness to retain a sufficient amount of oxygen therein, the device has no light-transparency on the support side, so that the color of the generated pigment should be measured from the sample-supplied side of the device. One object of the present invention is to provide an assay device which can overcome the above drawbacks of the conventional assay devices. Another object of the present invention is to provide an assay device which can quickly and easily analyze a particular component in a liquid sample with good accuracy. A further object of the present invention is to provide a process for analyzing a particular component in a liquid sample with using the assay device of the present invention. According to one aspect of the present invention, there is provided a device for colorimetric assay of at least one component in a liquid sample, which device comprises a support having therethrough a hole with an area of 3 to 80 mm², a transparent porous film which is provided on the support to cover the hole, a reagent layer provided on a surface of the porous film which is not contacted to the support, and a sample-holding layer which covers at least a part of the reagent layer and a part of a surface of the support. According to another aspect of the present invention, there is provided a process for analyzing at least one component in a liquid sample with using the assay device of the present invention, which process comprises steps of applying an amount of the liquid sample on the sample-holding layer to have the sample penetrated therein, and colorimetrically measuring a color developed in the reagent layer through the hole of the support from the support side of the device, for example, with a reflectometer. Fig. 1 is a cross section of a first embodiment of the assay device of the present invention, Fig. 2 is a cross section of a second embodiment of the assay device of the present invention, and Fig. 3 is a cross section of a third embodiment of the assay device of the present invention. The assay device of the present invention may be prepared as follows: A solution or dispersion of the reagent is applied on the transparent porous film and dried to form the reagent layer on the porous film. The composite of the porous film and the reagent layer is placed on the support with facing the porous film to the support to cover the hole in the support and fixed. Then, the sample-holding layer is provided to cover the reagent layer and fixed to the support. The porous film and the sample-holding layer may be fixed to the support, for example, by thermocompression bonding by using a thermoplastic resin, or with an adhesive double coating tape. Alternatively, only the porous film is firstly fixed to the support and then the reagent layer is formed on the porous film. The hole may be of any shape, for example, a round shape, a rectangular shape and the like. The support may have an additional hole at a position which is covered only with the sample-holding layer. Through the additional hole, the application of the sample on the sample-holding layer is detected from the support side of the device. This embodiment is advantageous in an automatic measurement since a period of time from the sample application to the measurement of the developed color can be kept constant. Over the sample-holding layer, another support having therethrough a hole may be provide, and the sample can be applied through such hole. The porous film to be used in the present invention preferably has a porosity of 2 to 70 %, a pore size of 0.01 to 20 µm and a thickness of 5 to 30 µm. Since such the porous film is air permeable, an amount of oxygen necessary for the oxidation reaction can be supplied to the reagent layer from the air. Although the porous film having a larger porosity and/or a larger thickness has air permeability, it has less transparency. Then, with such less transparent porous film, it is difficult or impossible to measure the color developed in the reagent layer through the porous film. Therefore, the transparent porous film to be used in the present invention preferably has a transparency of at least 30 %, more preferably at least 60 % for the visible light and near UV light, for example, light with a wavelength in the range from about 300 to about 900 nm. The porous film suitable for the present invention is commercially available under the trade name of Newclepore® (by Newclepore Ltd.) and Juraguard® (by Polyplastic Ltd.). Of course, other transparent oxygen-permeable films can be used in the present invention. The reagent layer contains the oxidase specific to the particular component to be analyzed, peroxidase and the pigment-precursor. Examples of the peroxidase and the pigment-precursor are already described above. The reagent layer may further contain light-reflecting water-insoluble particles, which prevents interference with colored components during the analysis of a colored sample such as blood and enables an accurate measurement from the porous film side. Preferably, the light reflecting water-insoluble particles have such a function that they prevent penetration of blood corpuscles into the reagent layer in the analysis of a blood sample. Preferred examples of the light reflecting water-insoluble particles are white pigments such as titanium oxide, zinc oxide, barium sulfate and magnesium oxide. Other particles which reflect light and are insoluble in the liquid sample can also be used. Examples of such particles are fluorocarbons, polystyrene latex particles, calcium carbonate, talc, alumina powder, dextran, acrylic polymer particles and the like. To improve an application property of the reagent liquid, it may contain a hydrophilic polymer. Specific examples of the hydrophilic polymer are hydroxypropylcellulose, methylcellulose, sodium alginate, polyvinyl alcohol, gelatin, modified gelatin, ager and the like. Penetrability of the reagent liquid is improved by the addition of an emulsion type adhesive or latex particles. The addition of at least one of nonionic surfactants, plasticizers and stabilizers to the reagent liquid improves the application property and the reactivity of the reagent liquid. The reagent layer may consists of two sub-layers one of which contains the enzyme and the color-developing agent and the other of which contains the light reflecting water-insoluble particles. The sample-holding layer spreads the applied sample over its entire surface and prevents the sample from being spilt out due to vibration and the like. The sample-holding layer is preferably made of a fibrous porous material such a filter paper, a fabric, a non-woven fabric and a mesh. The sample-holding layer may contain an anti-coagulating agent, ascorbate oxidase which prevents interference due to oxidation with possibly present ascorbic acid, or cholesterol esterase which converts an ester type cholesterol to a free cholesterol in the measurement of total cholesterols. The present invention will be illustrated by making reference to the accompanying drawings. Fig. 1 is a cross section of a first embodiment of the assay device of the present invention, which comprises a support 1 having therethrough a hole, a porous film 2 formed on the support to cover the hole, a reagent layer 3 fixed on the porous film and a sample-holding layer 4 fixed on the support to cover the reagent layer 3. The porous film 2 and the sample-holding layer 3 are fixed to the support with a thermoplastic resin or an adhesive double coating tape (not shown). Fig. 2 is a cross section of a second embodiment of the assay device of the present invention, which is substantially the same as the device of Fig. 1 except that another hole is provided in the support 1 at the position which is covered only with the sample-holding layer 4. For example, in the automatic measurement, the sample application is detected at the position indicated by the arrow A and after a predetermined period of time, the reflectance on the reagent layer 2 is measured at the position indicated by the arrow B. Fig. 3 is a cross section of a third embodiment of the assay device of the present invention, which comprises the porous film 2, the reagent layer 3 formed on the porous film 2 and the sample holding layer 4, all of which are fixed in a mount 5. The mount 5 has therethrough a hole on each of the porous film side and the sample-holding layer side. In Figs. 1, 2 and 3, the size in the thickness direction is enlarged for easy understanding. The present invention will be explained in detail by the following Examples. Example 1 Determination of glucose in blood On a transparent porous film (Newclepore®) having a thickness of 10 µm, a reagent liquid having the following composition was applied to a dry thickness of 100 µm and dried at 40°C for one hour followed by slitting to a width of 7 mm. Composition of the reagent liquid Glucose oxidase 10 ku Peroxidase 20 ku 4-Aminoantipyrine 150 mg N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline 200 mg 0.15 M phosphate buffer (ph 7.0) 2 ml 4 % Hydroxypropylcellulose solution 3 g 50 wt % Solution of titanium oxide 1 g On a support film having a series of holes therethrough each having a diameter of 4 mm and covered with a thermoplastic resin, the porous film sticks were placed with the reagent layer upside covering one of a pair of holes with each stick and thermocompression bonded. Over each of the other hole of a pair of holes and the respective reagent layers, strips of filter paper each having a width of 10 mm were placed and thermocompression bonded to the support. Then, along the lines between the adjacent two pairs of holes, the laminated film was cut to form assay devices. For comparison, an assay device was prepared by using a transparent non-porous film in place of the transparent porous film. On the sample-holding layer of each assay device, 20 µl of whole blood containing glucose in various concentrations was dropped. After one minutes, the reflectance at the wavelength of 630 nm on the reagent layer was measured from the porous film side through the hole with an integrating sphere type reflectometer. From the reflectance, the K/S value was calculated according to the simplified Kubelka-Munk equation: K/S = (1 - R)²/2 x R in which K is a constant, S is a scattering coefficient, and R is reflectance/100. The K/S value is linearly proportional to the molar concentration of glucose in the blood (cf. Gustav-Kortum, "Reflectance Spectroscopy", 106-111, Springer-Verlag, 1969). The results are shown in Table 1. Glucose concentration (mg/dl) Present invention Comparative Reflectance (%) K/S value Reflectance (%) K/S value 0 94.5 0.002 96.3 0.001 75 43.6 0.365 70.8 0.060 158 29.7 0.832 63.5 0.105 290 17.6 1.929 60.3 0.131 386 12.8 2.970 59.8 0.135 533 9.0 4.601 59.4 0.139 With the assay device of the present invention, the K/S value increased proportionally to the glucose concentration, while with the comparative assay device, the K/S value did not increase proportionally to the glucose concentration above the concentration of about 150 mg/dl. Example 2 On a transparent porous film (Juraguard®) having a thickness of 25 µm, a reagent liquid having the following composition was applied to a dry thickness of 200 µm and dried at 40°C for one hour. Then, the assay device was prepared in the same manner as in Example 1. Composition of the reagent liquid Lactate oxidase 990 u Peroxidase 23 ku 4-Aminoantipyrine 50 mg N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine 200 mg 0.3 M phosphate buffer (ph 7.0) 2 ml 4 % Hydroxypropylcellulose solution 3.7 g 50 wt % Solution of titanium oxide 1.2 g Nonionic surfactant (10 % Triton® X-100) 0.5 ml For comparison, an assay device was prepared by using a transparent non-porous film in place of the transparent porous film. On the sample-holding layer of each assay device, 20 µl of whole blood containing lactic acid in various concentrations was dropped. After one minutes, the reflectance at the wavelength of 560 nm on the reagent layer was measured from the porous film side through the hole with an integrating sphere type reflectometer, and the K/S value was calculated as in Example 1. With the blood plasma obtained from the whole blood by centrifugation, the same measurement was done. The results are shown in Table 2. Concentration of lactic acid Present invention Comparison Whole blood Plasma Whole blood Reflectance K/S value Reflectance K/S value Reflectance K/S value 9 66.8 0.083 63.8 0.103 72.8 0.051 20 50.6 0.241 48.7 0.270 58.6 0.146 43 32.8 0.688 32.0 0.723 48.3 0.277 75 20.3 1.565 18.8 1.754 41.0 0.425 97 15.0 2.408 13.9 2.667 39.3 0.469 108 13.2 2.854 12.3 3.127 38.7 0.485 With the assay device of the present invention, the K/S value increased proportionally to the lactic acid concentration both for the whole blood and the plasma, while with the comparative assay device, the K/S value did not increase proportionally to the lactic acid concentration above the concentration of about 75 mg/dl.

Claims: A device for colorimetric assay of at least one component in a liquid sample, which device comprises a support having therethrough a hole with an area of 3 to 80 mm², a transparent porous film which is provided on the support to cover the hole, a reagent layer provided on a surface of the porous film which is not contacted to the support, and a sample-holding layer which covers at least a part of the reagent layer and a part of a surface of the support. The device according to claim 1, wherein the reagent layer contains an oxidase which oxidize said at least one component, peroxidase and a pigment-precursor. The device according to claim 1, wherein the porous film has a porosity of 2 to 70 %. The device according to claim 1, wherein the porous film has a pore size of 0.01 to 20 µm. The device according to claim 1, wherein the porous film has a thickness of 5 to 30 µm. The device according to claim 1, wherein the reagent layer further contains light reflecting insoluble particles. The device according to claim 1, wherein the support has therethrough an additional hole at a position which is covered only with the sample-holding layer. The device according to claim 1, which further comprises a support having therethrough a hole over the sample-holding layer. A process for analyzing at least one component in a liquid sample using the assay device of claim 1, which process comprises steps of applying an amount of the liquid sample on the sample-holding layer to have the sample penetrated therein, and colorimetrically measuring a color developed in the reagent layer through the hole of the support from the support side of the device.

Inventors: SAKAMOTO HISASHI, TANIGUCHI HIROSHI, YAMADA SHIGEKI, SAKAMOTO, HISASHI, TANIGUCHI, HIROSHI, YAMADA, SHIGEKI
Applicants: KYOTO DAIICHI KAGAKU KK, KYOTO DAIICHI KAGAKU CO., LTD.
Application year: 1989
Main classification: G01N33
IPC codes: G01N33, G01N21, G01N31