Method of detecting bitter or odor substances and apparatus therefor

Abstract: Disclosed are a method of detecting bitter or odor substances, which method comprises adsorbing the bitter or odor substances onto an immobilized bilayer film and directly, quantitatively, separately, and selectively detecting the adsorbed bitter or odor substances, and an apparatus therefor.

Description: This invention relates to a method of quantitatively detecting bitter or odor substances and an apparatus therefor. No method of directly detecting bitter or odor substances has been proposed so far, but the bitter or odor substances have been sensed by gustatory cells in humans. A method of detecting odor substances by gas chromatography has been known with so many peaks as to be difficult to analyze. Reactive sensors have been practically used for special malodorants among odor substances such as detection of alcohol by use of metal oxide and detection of propane gas by reaction with -SH group. However, no method of directly and quantitatively detecting a large number of odor substances including the above malodorants has been known yet. One thousand or more bitter and odor substances exist, and it is highly demanded to develop any method of selectively detecting them. In (japanese journal) "Science, 54 , No. 11, pages 669-678 (1984), Kenzo Kurihara", for example, a mechanism has been proposed, in which bitter substances are adsorbed onto the lipid bilayer moiety of the micro-villi membrane (hereinafter referred to as the taste receptor membrane) consisting of the lipid bilayer and various kinds of proteins in taste cells with the result that a cell potential of the taste cell is depolarised to release transfer substances and that the transfer substance acts on the taste nerve end to generate impulse for sensing bitter substances. On the other hand, a mechanism has been proposed, in which odor substances are adsorbed onto the lipid bilayer moiety of the olfactory receptor membrane composed of the lipid bilayer and various kinds of proteins in olfactory cells with the result that depolarization takes place and impulse is generated from the olfactory nerve to sense odor substances. Examples of methods previously used for detecting odorous substances, including methods which employ crystal oscillators are disclosed in Journal of Physics E Scientific Instruments, Vol. 19, No. 4, April 1986 pp 271-278, and DE 1673104 (ETILER T. N. and FAITH, Y. G.). In addition, Analytical Chemistry, Vol. 58, No. 6, May 1986, pp 1206-1209 discloses deposition of Langmuir Blodgett films of stearic acid and immobilised antigen on such crystals for detection of antibodies in solution. This invention seeks to provide a method of directly, quantitatively, selectively and easily detecting bitter or odor substances without effecting the conventional organoleptic test, and a simple and effective apparatus therefor. This invention also aims to provide a method of detecting bitter or odor substances and an apparatus therefor, which are capable of separately and selectively detecting respective components of a mixture of bitter or odor substances in real time with pattern recognition by use of a plurality of simple and effective appartatuses therefor. This invention further seeks to provide a method of detecting bitter or odor substances and an apparatus therefor, which are capable of quantitatively detecting bitter or odor substances with precision in the odor of 1 to 10 nanograms by a simple method and apparatus. That is, the present invention provides a method of detecting bitter or odor substances, which method comprises adsorbing the bitter or odor substances onto an immobilized bilayer film and detecting the adsorbed bitter or odor substances according to the method as set out in Claim 1. The present invention also provides an apparatus for detecting bitter or odor substances, as set out in Claim 2. Brief Description of the Drawings : Fig. 1 is a schematic view of an apparatus for measuring membrane potential and/or membrane resistance in a first preferred embodiment of the present invention, Fig. 2 is a graph showing the result of the measurement of the membrane potential, Fig. 3 is a graph showing the result of the measurement of the membrane resistance, Fig. 4 is a graph showing correlation between threshold concentration of adsorption for bitter substances and threshold value in humans, Fig. 5 is a graph showing correlation between threshold concentration of adsorption for odor substances and threshold value in frog. Fig. 6 is a schematic view of an apparatus for measuring a decreased amount in frequency of the crystal oscillator in a second preferred embodiment of the present invention, Fig. 7 is a graph showing changes with time of frequency changes and adsorption amounts for bitter substances, Fig. 8 is a graph showing changes with time of frequency changes and adsorption amounts for odor substances. Fig. 9 is a graph showing changes with time of frequency changes and adsorption amounts for gaseous odor substances, Fig. 10 is a graph showing correlation between partition coefficient and olfactory threshold value in humans. Fig. 11 is a graph showing correlation between partition coefficient and perfume intensity. Fig. 12 is a graph showing correlation of adsorbed amount and frequency change to temperature, Fig. 13 is a graph showing correlation of adsorbed amount and frequency change to concentration. Fig. 14 is a graph showing correlation between partition coefficient and potency, Fig. 15 is a graph showing correlation between diffusion coefficient and potency, Fig. 16 is a graph showing correlation between threshold value and potency. Fig. 17 and Fig. 18 are graphs showing correlation between partition coefficient and potency respectively, Fig. 19 is a graph showing correlation of adsorption amount and frequency change to thickness of bilayer films. Fig. 20 is a graph showing correlation between temperature and partition coefficient, Fig. 21 is a graph showing correlation of partition coefficient and diffusion coefficient to temperature. Fig. 22 is a graph showing correlation between partition coefficient and potency, Fig. 23 is a graph showing correlation of adsorption amount and frequency change to thickness of bilayer films. Fig. 24 and Fig. 25 are graphs showing correlation between temperature and partition coefficient respectively. Detailed Description : The bitter substances used in the present invention are not specifically limited so long as they are adsorbed onto the immobilized bilayer film of the present invention, and may include acids, salts, etc. The typical examples of the bitter substances include strychnine, quinine, nicotine, phenylthiourea, papaverine, caffeine, naringin, octaacetyl sucrose, and the like. The odor substances used in the present invention are not specifically limited so long as they are adsorbed onto the immobilized bilayer film of the present invention, and may include perfumes, anesthetics, malodorants, and the like. The typical examples of the odor substances include β-ionone, aliphatic alcohols such as octanol, camphor, amylacetate, vanilline, ethylbutylate, phenol, aldehydes, and the like. Typical examples of the perfumes include P-anisaldehyde, l-undecanol, anisalcohol, anisol, phenylethyl acetate, citral, methyl salicylate, benzyl acetate, tetrahydrogeraniol, terpineol, geranyl acetate, and the like. Examples of the general anesthetics as the compounds having narcosism in the aforementioned anesthetics are shown in Table 3. In Table 3, potency is a value representing intensity of the anesthetics and is shown as values obtained by use of a tadpole. No. Anesthetic Compounds Potency 1 methanol 1.00 2 ethanol 2.43 3 acetone 3.47 4 l-propanol 9.43 5 butanone 1.20 x 10 6 diethyl ether 2.99 x 10 7 l-butanol 4.43 x 10 8 paraldehyde 5.44 x 10 9 benzylalcohol 5.01 x 10² 10 chloroform 7.62 x 10² 11 l-hexanol 1.12 x 10³ 12 halothane 4.47 x 10³ 13 methoxyflurane 4.86 x 10³ 14 l-octanol 7.93 x 10³ 15 pentane 1.51 x 10⁴ 16 l-nonanol 4.03 x 10⁴ 17 hexane 6.75 x 10⁴ 18 l-decanol 1.00 x 10⁵ Examples of the malodorants include malodor-emitting substances selected from ketones, amines, imines, aldehydes such as acetaldehyde, organic acids and the like, sulfur compounds such as methyl mercaptan, hydrogen sulfide, methane sulfide, methyl disulfide and the like, styrene, mixtures thereof, malodor-emitting substances selected from various kinds of industrial wastes and mixtures thereof, foul breath-producing substances and mixtures thereof, and the like. The immobilised bilayer films used in the present invention comprise those obtainable by immobilising, by use of polymers, (i) synthetic lipids selected from ammonium salts, sulfonates, carboxylates in the form of trialkyl, dialkyl and/or monoalkyl as represented by the formula: a-C m -X-C n , a-C m -X-C n or a-C m -X-C n -b where a and b represent a hydrophilic group moiety selected from -N⁺(CH₃ )₃, -SO₃⁻, PO₄⁻, polyol, and polyether, C₁, C m and C n represent a hydrophobic group moiety selected from an alkyl group, fluoroalkyl group, and an alkylene group having a C₈ or higher carbon chain, and X represents a rigid segment selected from a diphenylazomethylene group, biphenyl group, naphthalene group, and anthracene group; and/or (ii)a natural lipids selected from phosphatidyl choline and phosphatidyl serine. Specific examples of the immobilised bilayer film used in the present invention include (i) films blending the synthetic lipid and/or natural lipid with high-molecular compounds such as polyvinyl alcohol, acetyl cellulose and the like, followed by casting; (ii) films prepared by impregnating pores of filters having a microporous structure such as miliporefilter, and the like, with a chloroform solution of the synthetic lipid and/or natural lipid, followed by drying; (iii) films obtained by dissolving a polyion complex powder prepared by mixing an aqueous dispersion of the synthetic lipid and/or natural lipid having a cationic, hydrophilic group with an aqueous solution of an anionic high-polymer such as polystyrene sulfonic acid, heparin, polyvinylsulfonic acid, polyacrylic acid, polyglutamic acid, and the like in chloroform, followed by casting; (iv) polyion complex type bilayer films composed of the lipid having an anionic hydrophilic group and cationic high polymer such as polyallylamine, polyethylene imine, quaternary polyaminostyrene and the like; and (v) Langmuir-Blodgett type multibilayer films composed of the synthetic lipid and/or natural lipid. The crystal oscillator or the piezoelectric crystal used in the present invention may include the crystal oscillator or the piezoelectric crystal conventionally used as a sensor for sulfur dioxide, ammonia gas, etc., and SAW device. A microbalance having a sensitivity of the order of nanogram may also be used in place of the crystal oscillator or the piezoelectric crystal. Thus, the present invention makes it possible to directly, quantitatively, selectively and easily detect bitter or odor substances without effecting the conventional organoleptic test. The present invention also seeks to make it possible to directly, quantitatively, selectively and easily detect odor substances both in the aqueous phase and gas phase in place of the conventional method using living bodies as samples and living things for detection of odor substances. The present invention also seeks to make it possible to quantitatively detect bitter or odor substances with precision in the order of 1 to 10 nanograms by a simple method and apparatus. The present invention also aims to make it possible to separately and selectively detect respective components of a mixture of bitter or odor substances in real time with pattern recognition by use of a plurality of simple and effective apparatuses therefor. The present invention will be described more in detail with reference to drawings and Examples. A first embodiment of the apparatus for detecting bitter or odor substances in the present invention is explained with reference to Fig. 1. In Fig. 1, figure 1 is an immobilized bilayer film, which is mounted at the end of polyethylene tube 2, for example, and is arranged between an inner aqueous phase 6 free of bitter or odor substances and an outer aqueous phase containing bitter or odor substances injected from bitter or odor substances injecting device 5, and both measuring probes of the galvanostat for means of measuring the membrane potential or membrane resistance are inserted into the outer aqueous phase 4 and the inner aqueous phase 6 respectively. In Fig. 1, figure 3 is a cell containing outer aqueous phase 4. The membrane potential is measured, for example, by use of a Ag/AgCl/KCl sat electrode with the inner aqueous phase 6 containing 5 mM NaCl and the outer aqueous phase 4 containing 0.5 mM NaCl. The membrane resistance is measured, for example, by LCR meter by use of a platinum electrode with the inner and outer aqueous phases both containing 0.1 M NaCl. A second embodiment of the apparatus for detecting bitter or odor substances in the present invention is explained with reference to Fig. 6. In Fig. 6, the immobilized bilayer film is cast on a silver-deposited electrode 2 of the crystal oscillator 1 [or on both sides of a silver-electrode-deposited piezoelectric crystal (9 MHZ; AT-cut)] and dipped into deionized water in cell 3, bitter or odor substances are injected thereinto as a solution thereof through an irritant-injecting device 4, frequency changes after the injection are measured by a frequency measuring means (not shown) constituting the crystal oscillator 1, and adsorbed amounts of bitter or odor substances onto the immobilized bilayer film are determined from proportionality thereof to the frequency changes thus obtained. Selectivity of bitter or odor substances to types of the bilayer constituting the immobilized bilayer film makes it possible to separately and selectively detect respective components of a mixture of bitter or odor substances in real time with pattern recognition by use of a plurality of detecting apparatuses comprising immobilized bilayer films having different bilayers respectively. Example 1 An example of the method of detecting bitter or odor substances in the present invention is more specifically explained with reference to Figs. 1-5. In Fig. 1, an immobilized bilayer film 1 obtained by casting a polyion complex between dialkyl ammonium salt ion (2C₁₈N⁺2C₁) and polystyrene sulfonic acid ion (PSS⁻) [or an immobilized 2C₁₈N⁺2C₁/PSS⁻ bilayer film 1 prepared as a polyion complex from dioctadecyldimethylammonium bromide 2C₁₈N⁺2C₁Br⁻ and sodium poly(styrenesulfonate) (PSS⁻Na⁺)] is cast from chloroform solution on both sides of a silver-electrode-deposited piezoelectric crystal 2 (9 MHZ; AT-cut). The cast film is mounted at the end of a polyethylene tube 2 and dipped into deionized water in 50 ml cell 3. Bitter or odor substances are injected into the outer aqueous phase 4 through irritant-injecting device 5 and the resulting membrane potential is measured by use of a Ag/AgCl/KCl sat electrode with the inner aqueous phase 6 containing 5 mM NaCl and the outer aqueous phase 4 containing 0.5 mM NaCl. The membrane resistance is measured by LCR meter by use of a platinum electrode with the inner and outer aqueous phases both containing 0.1 M NaCl. Papaverine as the bitter substance and octanol as the odor substance are added at varied concentrations respectively, and the resulting membrane potential changes and membrane resistance changes are shown in Fig. 2 and Fig. 3 respectively. Figs. 2 and 3 show that addition of the bitter substances such as papaverine results in little or no changes in membrane resistance in the measured concentration range of 10⁻⁶ - 10⁻³M, and that the membrane potential only changes at concentrations of 10⁻⁵M or higher. This shows that the membrane potential change due to adsorption of bitter substances is mainly caused by interfacial potential. On the other hand, addition of odor substances such as octanol results in simultaneous changes in membrane potential and membrane resistance in the concentration range of 10⁻⁴M or higher. This suggests that adsorption of odor substances such as octanol results in disturbing the structure of the bilayer film to reduce membrane resistance, and that membrane potential changes are produced by diffusion potential changes. As shown in Fig. 4, the threshold concentrations causing membrane potential changes for bitter substances by use of the immobilized bilayer film correspond very well to threshold values in humans. As shown in Fig. 5, the threshold concentrations causing membrane potential changes for odor substances correspond very well to membrane potential-producing threshold values of olfactory cells in frog. This makes it possible to detect bitter substances by adsorbing bitter substances onto the immobilized bilayer film, for example, followed by measuring the resulting membrane potential changes due to the adsorption, and to detect odor substances by adsorbing odor substances onto the immobilized bilayer film, followed by measuring changes in membrane potential and/or membrane resistance due to the adsorption. The immobilized bilayer film is prepared, for example, by a process in which dialkylammonium salt ion and polystyrenesulfonic acid ion are reacted at 70°C to form precipitates of polyion complex followed by reprecipitation and drying, and the resulting precipitates are dissolved in chloroform to be cast on a substrate. Example 2 As shown in Fig. 6, the same immobilized 2C₁₈N⁺2C₁/PSS⁻ bilayer film as in Example 1 is cast from chloroform solution on both sides of a silver-electrode deposited piezoelectric crystal 2 (9 MHZ; AT-cut) or on a silver-deposited electrode 2 of the crystal oscillator 1 to a thickness of 0.5 µm, followed by being dipped into deionized water in 50 ml cell 3. An ethanol solution of strychnine as the bitter substance or of β-ionone as the odor substance is injected through an irritant-injecting device 4 to measure frequency changes due to injection, and to determine adsorbed amounts onto the immobilized bilayer film from proportionality thereof to frequency changes respectively. Measurements are made at 45°C and a concentration of 58.3 µM for strychnine, and at 45°C and a concentration of 125 µM for β-ionone. Fig. 7 and Fig. 8 show frequency changes and adsorption amounts calculated therefrom with time respectively. As shown in Fig. 7 and Fig. 8, the crystal oscillator is removed from the cell and dipped into distilled water in a separate cell 200 seconds after starting the measurements with the result that desorption of the adsorbed strychnine or β-ionone is observed. This shows that adsorption of bitter substances such as strychnine and that of odor substances such as β-ionone are both reversible, and that the crystal oscillator may be used repeatedly because of easy adsorption thereonto or desorption therefrom. On the other hand, the same correlation as shown in Fig. 4 is observed between gustatory threshold values in humans and threshold concentrations (C th ), at which frequency change due to adsorption of bitter substances onto the immobilized bilayer film is observed. The same correlation as shown in Fig. 5 is observed between olfactory threshold values in frog and threshold concentrations (C th ), at which frequency change due to adsorption of odor substances onto the immobilized bilayer film is observed. The threshold concentrations (C th ) are specific to respective bitter substances or odor substances relative to changes in frequency of the crystal oscillator. Example 3 The experiments in Example 2 are repeated except that the immobilized bilayer film is in the state of liquid crystals with the result that adsorbed amounts are increased. Example 4 Adsorbed amounts of strychnine as the bitter substance, β-ionone as the odor substance, sugar as the sweet substance and of glycine as the delicious substance are measured respectively in the same manner as in Example 2, and the adsorbed amounts thus measured are shown in Table 1 in terms of partition coefficient. The partition coefficient means a ratio of the adsorbed amount of an irritant such as the bitter or odor substance or the like onto the immobilized bilayer film to an amount by weight of the irritant such as the bitter or odor substance or the like in the aqueous solution. The results in Table 1 show that adsorption onto the immobilized bilayer film of the present invention is specific and selective to the bitter and odor substances. Irritants Partition Coefficient (10⁻³) Strychnine 2.5 β-ionone 3.0 Sugar 0.01 Glycine 0.02 Example 5 Experiments are carried out in the same manner as in Example 2 by use of strychnine as the bitter substance in the cases where the immobilized bilayer film, hydrophobic polystyrene (PSt) film, hydrophilic polyvinyl alcohol (PVA) film and polymethyl-L-glutamate (PMLG) film as polyamino acid are coated on the silver-deposited electrode 2 of the crystal oscillator or on the silver-electrode-deposited piezoelectric crystal 2 respectively, and where none are coated to measure the adsorbed amounts, and the results are shown in Table 2 in terms of partition coefficient. The results in Table 2 show that strychnine as the bitter substance is specifically and selectively adsorbed onto the immobilized bilayer film of the present invention. Coating Partition Coefficient (10⁻³) None 0.03 Immobilized bilayer film 2.5 Polystyrene film 0.05 Polyvinyl alcohol film 0.03 Polymethyl-L-glutamate film 0.02 Example 6 The same immobilized bilayer film-coated crystal oscillator or crystal as in Example 2 is placed at point A in Fig. 9 in a cell saturated with β-ionone vapor as the odor substance in place of the cell containing deionized water as in Example 2, and the frequency changes are measured as in Example 2 to determine the adsorbed amounts. The results are shown in Fig. 9. As shown in Fig. 9, as soon as the coated crystal oscillator or crystal is placed in the cell, the frequency rapidly decreases and then becomes constant soon. At point B in Fig. 9, the coated crystal oscillator or crystal is removed from the cell into atmosphere with the result that the frequency reverts soon to the same as at point A in Fig. 9, at which the coated crystal oscillator or crystal is placed in the cell. The same experiments as above are repeated for n-octanol, isoamylacetate and ether as other odor substances with the same results as above. In the determination of adsorbed amounts from frequency changes thus obtained, the adsorbed amount is represented in terms of partition coefficient obtained by dividing the adsorbed amount by the total weight of the odor substance molecules actually existing in the gas phase in order to cancel differences in vapor pressure in the gas phase. Fig. 10 shows a good correlation between the partition coefficient thus obtained and olfactory threshold values in humans. Comparative Example 1 The same experiments as in Example 6 are repeated except that an uncoated crystal oscillator or crystal is used with the result that little or no frequency changes due to adsorption of odor substances are observed. Comparative Example 2 The same experiments as in Example 6 are repeated except that a crystal oscillator or crystal coated with polystyrene as the hydrophobic polymer with the result that very little frequency changes due to adsorption of odor substances are observed. Example 7 The same experiments as in Example 6 are repeated by use of benzyl acetate, phenethyl acetate, citral, anisol, p-anisaldehyde and l-undecanol selected from perfumes as odor substances. The results are shown in Fig. 11. Fig. 11 shows a good correlation between logarithm of partition coefficient (log P) and perfume intensity relative to the immobilized bilayer film. The perfume intensity means an intensity determined in such a manner that the perfumer sniffs at odors emitted from respective dialkylphthalate solutions of perfumes and empirically determines intensities of respective primary odors on the basis of citral as the perfume. Example 8 Experiments are repeated in the same manner as in Example 2 to show the temperature dependence of the frequency change and adsorption amount of strychnine as the bitter substance in the aqueous solution containing 19.3 p.p.m. of strychnine. The results are shown in Fig. 12. Fig. 12 shows that adsorption on the 2C₁₈N⁺2C₁/PSS⁻ bilayer-coated crystal or crystal oscillator is high and specifically increased near 40-45°C, close to the phase transition temperature (Tc = 45°C) of the lipid bilayer on the crystal or the crystal oscillator. Similar temperature dependence is observed on addition of various bitter substances other than strychnine and odor substances, and that these substances are specifically adsorbed at temperatures near T c , where solids coexist with fluid liquid crystals in the lipid bilayers. Comparative Example 3 The same experiments as in Example 8 are repeated except for using an uncoated crystal oscillator or piezoelectric crystal with the result that little or no frequency changes due to adsorption of strychnine are observed regardless of the temperature employed. The results are shown in Fig. 12. Similar temperature dependence is observed on addition of various bitter substances other than strychnine and odor substances. Comparative Example 4 The same experiments as in Example 8 are repeated except for using a hydrophobic polystyrene-coated crystal oscillator or piezoelectric crystal with the result that very little frequency changes due to adsorption of strychnine are observed regardless of the temperature employed. The results are shown in Fig. 12. Similar temperature dependence is observed on addition of various bitter substances other than strychnine and odor substances. Example 9 Experiments are repeated in the same manner as in Example 2 at 45°C in the state of liquid crystals and at 20°C in the crystalline state respectively in an aqueous solution of halothane as the anesthetics at a concentration of 9.48 x 10⁻⁴(M). The results are shown in Table 4. The results show that adsorbed amounts are increased when the coated film is in the state of liquid crystals. Example 10 Experiments of Example 9 are repeated except that cholesterylammonium poly(p-styrenesulfonate) is used as the immobilized bilayer film. The results are shown in Table 4. Example 11 Experiments of Example 9 are repeated except that cetyl trimethylammonium poly(p-styrenesulfonate) (CTAB⁺/PSS⁻) is used as the immobilized bilayer film. The results are shown in Table 4. Comparative Example 5 Experiments of Example 9 are repeated at 45°C except that a hydrophobic polystyrene film in place of the 2C₁₈N⁺2C₁/PSS⁻ film is used as the coated film. The results are shown in Table 4. Comparative Example 6 Experiments of Example 9 are repeated at 45°C except that a hydrophobic polyvinylchloride film in place of the 2C₁₈N⁺2C₁/PSS⁻ film is used as the coated film. The results are shown in Table 4. Comparative Example 7 Experiments of Example 9 are repeated at 45°C except that a hydrophilic polyvinyl alcohol film in place of the 2C₁₈N⁺2C₁/PSS⁻ film is used as the coated film. The results are shown in Table 4. Comparative Example 8 Experiments of Example 9 are repeated except that a film of poly(γ-methyl-L-glutamate) as polyamino acid in place of the 2C₁₈N⁺2C₁/PSS⁻ film is used. results are shown in Table 4. Example 12 Experiments of Example 2 are repeated by using 18 anesthetics as shown in Table 3 respectively at varied concentrations. The results are shown in Fig. 13. Respective figures in Fig. 13 represent the numbers of respective anesthetic compounds. Since frequency changes of 10 Hz or less are considered to be within errors in measurements, the concentration at the frequency change of 10 Hz is defined as a concentration threshold value. As shown in Fig. 13, as the concentration is increased, the adsorbed amounts linearly increase, and this means that the partition coefficient shows a constant value above the threshold value. Correlation between the logarithm of the partition coefficient thus obtained and the logarithm of potency of respective anesthetics shown in Table 3 is shown in Fig. 14. Similarly, correlation between the logarithm of diffusion coefficient D and the logarithm of potency in the tadpole is shown in Fig. 15. Correlation between the logarithm of the threshold value and the logarithm of potency in the tadpole is shown in Fig. 16. Fig. 14 shows a good correlation (coefficient of correlation γ = 0.988) between potency and partition coefficient. This shows that the anesthetic having increased partition coefficient has increased intensity as the anesthetic too, and it may be said that this strongly suggests production of narcosism by direct adsorption of the anesthetic onto the lipid film. Example 13 Adsorption experiments are carried out in the same manner as in Example 12 at 45°C in the state of liquid crystals and 20°C in the crystalline state. Correlation between the logarithm of partition coefficient P and the logarithm of potency in the tadpole is shown in Fig. 17. Fig. 17 shows a good correlation (coefficient of correlation γ = 0.961) between potency and partition coefficient at 20°C in the crystalline state, but shows that the above correlation is poor compared with that (coefficient of correlation γ = 0.988) at 45°C in the state of liquid crystals, and that the partition coefficients at 20°C are less, as a whole, than those at 45°C. It may be said that this suggests direct action of the anesthetic onto the lipid in the membrane of the living body in the state of liquid crystals. Comparative Example 9 Adsorption experiments of anesthetics in the same manner as in Example 12 by use of a hydrophobic polystyrene film in place of the 2C₁₈N⁺2C₁/PSS⁻ film. Correlation between partition coefficient P and potency in the tadpole is shown in Fig. 18 along with the results in Example 12. Coefficient of correlation for the polystyrene film is 0.901 and less than that (0.988) for the 2C₁₈N⁺2C₁/PSS⁻ film, resulting in poor correlation compared with that for the 2C₁₈N⁺2C₁/PSS⁻ film. Example 14 Adsorption experiments are carried out in the same manner as in Example 2 at 45°C with varied film thickness of the 2C₁₈N⁺2C₁/PSS⁻ cast film by use of halothane as the anesthetic at a concentration of 3.00 x 10⁻⁴(M). The results are shown in Fig. 19. Fig. 19 shows that the adsorbed amount of halothane is increased with increased film thickness, and that halothane penetrates into the film to be adsorbed. Example 15 Adsorption experiments are carried out in the same manner as in Example 2 at varied temperatures by use of halothane as the anesthetic at a concentration of 9.5 x 10⁻⁴(M) in the aqueous solution. The results are shown in Fig. 20 in the form of an Arrhenius plot. Fig. 20 shows that the partition coefficient of halothane onto the coated film has a maximum at the phase transition temperature (Tc) of the 2C₁₈N⁺2C₁/PSS⁻ cast film, i.e. in the state of liquid crystals. An Arrhenius plot of the diffusion coefficient D obtained is shown in Fig. 21. Fig. 21 shows that the diffusion coefficient D of the 2C₁₈N⁺2C₁/PSS⁻ film critically changes near a temperature at which the phase transition from crystals to liquid crystals starts to take place, and it is considered that the diffusion therein is increased in the state of liquid crystals. Comparative Example 10 The same adsorption experiments as in Example 15 are carried out except for using a polystyrene film in place of the 2C₁₈N⁺2C₁/PSS⁻ film. The results are shown in Fig. 20, resulting in showing quite a different adsorption behavior from the case where the 2C₁₈N⁺2C₁/PSS⁻ film is used. Examples 16-18; Comparative Examples 11-16: Adsorption experiments are carried out in the same manner as in Example 2 in gas phase by use of various cast films as shown in Table 5 and by using 2 µg of halothane according to the inject process under the conditions of a film weight of 20 µg and a temperature of 25°C. The results are shown in Table 5. Table 5 shows that the partition coefficients in the gas phase fairly well correspond to those in the aqueous phase as shown in Table 4. Coated films Partition Coefficients (P) Polyion complex film Example 16 2C₁₈N⁺2C₁/PSS⁻ 117 Example 17 Cholesterylammonium poly(p-styrenesulfonate) 12.7 Example 18 CTAB⁺/PSS⁻ 79.6 Hydrophobic polymer Comparative Example 11 Polystyrene [Pst] 16.9 Comparative Example 12 Polyvinylchloride [PVC] 10.2 Hydrophilic polymer Comparative Example 13 Polyvinyl alcohol [PVA] 0 Polyamino acid Comparative Example 14 Poly(γ-methyl-L-glutamate) [PMLG] 19.4 Membrane protein Comparative Example 15 Bacteriorhodopsin 0 Comparative Example 16 Luciferase 0 Example 19 Adsorption experiments are carried out in the gas phase at 25°C by use of the same 2C₁₈N⁺2C₁/PSS⁻ cast film as in Example 2 for 18 anesthetics shown in Table 6. The results are shown in Fig. 22. In Table 6, "potency" means a value determined by a concentration ratio of the aqueous solution of anesthetics in the aqueous system taking that of methanol as 1.00, whereas "potency (atm)" means a value obtained by converting "potency" to be used in the gaseous system by determining the partial pressure of the anesthetic in the gas phase from the concentration in the aqueous solution thereof followed by determining the partial pressure ratio taking that of methanol as 1.00. Fig. 22 shows a fair correlation between potency (atm) and partition coefficient P, although not so good as in the aqueous system of Example 12. The above results mean that the anesthetics must be taken into the living body in the aqueous phase in order to be used in real time in the clinical cases, whereas in the gas phase, the detection of the anesthetics provided in the gas phase is satisfactory, resulting in being used all the time, and, if necessary, the potency of the anesthetic may roughly but simply be determined from the partition coefficient in the gas phase system. The above results further mean that a concentration of an anesthetic compound having a known partition coefficient in the gas phase may be determined from the adsorbed amount by use of the bilayer film coated crystal oscillator, resulting in being useful when an accurate concentration of the anesthetic in the gas phase is required in biological experiments, and in applications to further developments of the anesthetics. No. Anesthetic compounds Potency Potency atm 1 methanol 1.00 1.00 2 ethanol 2.43 5.19 3 acetone 3.47 1.76 4 l-propanol 9.43 4.42 x 10 5 butanone 1.20 x 10 1.51 x 10 6 diethyl ether 2.99 x 10 6.43 7 l-butanol 4.43 x 10 9.41 x 10² 8 paraldehyde 5.44 x 10 8.20 x 10² 9 benzyl alcohol 5.01 x 10² 6.25 x 10⁵ 10 chloroform 7.62 x 10² 4.62 x 10² 11 l-hexanol 1.12 x 10³ 2.23 x 10⁵ 12 halothane 4.47 x 10³ 1.72 x 10³ 13 methoxyflurane 4.86 x 10³ 1.82 x 10⁴ 14 l-octanol 7.93 x 10³ 1.21 x 10⁷ 15 pentane 1.51 x 10⁴ 3.34 x 10³ 16 l-nonanol 4.03 x 10⁴ 2.51 x 10⁸ 17 hexane 6.75 x 10⁴ 5.21 x 10⁴ 18 l-decanol 1.00 x 10⁵ 1.11 x 10⁹ Example 20 Adsorption experiments are carried out in the gas phase at 25°C by use of the same 2C₁₈N⁺2C₁/PSS⁻ cast film as in Example 2 and of 2 µℓ of halothane as the anesthetic varying the film thickness. The results are shown in Fig. 23. Fig. 23 shows that as the film thickness is increased, the adsorption amount is linearly increased, and this shows that halothane penetrates into the cast film. Example 21 Adsorption experiments are carried out in the gas phase by use of the same 2C₁₈N⁺2C₁/PSS⁻ cast film as in Example 2 and of 2 µℓ of halothane as the anesthetic at varied temperatures. The results are shown in Fig. 24 along with the results in the aqueous system of Example 15 in the form of an Arrhenius plot. Fig. 24 shows that no phase transition effect is observed in the gas phase differently from that in the aqueous system. Example 22 The same adsorption experiments as in Example 21 are repeated except for using β-ionone as the odor substance in place of halothane as the anesthetic. The results are shown in Fig. 25 along with the results of halothane. Fig. 25 shows that a discontinuous Arrhenius plot is observed near the phase transition temperature Tc. Examples 23-27 Adsorption experiments are carried out by use of the same crystal oscillator or piezoelectric crystal as in Example 2 and of the bitter or odor substances shown in Table 7 for five different immobilized bilayer films shown in Table 7. The results are shown in Table 7 in terms of partition coefficient as in Example 4. The results in Table 7 shows that adsorbed amounts of respective components of a mixture of bitter and/or odor substances may be separately and selectively determined in real time by use of a plurality of detecting apparatuses having different types of immobilized bilayer films respectively.

Claims: A method of detecting bitter or odor substances, which method comprises adsorbing the bitter or odor substances onto an immobilized bilayer film, characterised in that the bilayer film is selected from films obtainable by immobilising, by use of polymers (i) a synthetic lipid selected from the group consisting of ammonium salts, sulfonates, carboxylates in the form of triaalkyl, dialkyl and/or monoalkyl as represented by the formula: a-C m -X-C n , a-C m -X-C n or a-C m -X-C n -b where a and b represent a hydrophilic group moiety selected from -N⁺(CH₃)₃, -SO₃⁻, PO₄⁻, polyol, and polyether, C₁, C m and C n represent a hydrophobic group moiety selected from an alkyl group, fluoroalkyl group, and alkylene group having a C₈ or higher carbon chain, and X represents a rigid segment selected from a diphenylazomethylene group, biphenyl group, naphthalene group, and anthracene group; and/or (ii) a natural lipid which is selected from phosphatidyl choline and phosphatidyl serine; and in that either (a) the resulting changes in membrane potential and/or membrane resistance of the bi-layer film due to adsorbtion of the bitter or odor substances are measured to detect the bitter or odor substances, or (b) in the case where the immobilized bilayer film is cast on an electrode of a crystal oscillator or a piezoelectric crystal, decreases in frequency of the crystal oscillator or the piezoelectric crystal due to the adsorption of the bitter or odor substances are measured to detect the bitter or odor substances. An apparatus for use in detecting bitter or odor substances, which apparatus comprises an immobilized bilayer film to adsorb the bitter or odor substances thereonto characterised in that the bilayer film is selected from the group consisting of films obtainable by immobilising, by use of polymers, (i) a synthetic lipid selected from ammonium salts, sulfonates, carboxylates in the form of triaalkyl, dialkyl and/or monoalkyl as represented by the formula: a-C m -X-C n , a-C m -X-C n or a-C m -X-C n -b where a and b represent a hydrophilic group moiety selected from -N⁺(CH₃)₃, -SO₃⁻, PO₄⁻, polyol, and polyether, C₁, C m and C n represent a hydrophobic group moiety selected from an alkyl group, fluoroalkyl group, and alkylene group having a C₈ or higher carbon chain, and X represents a rigid segment selected from a diphenylazomethylene group, biphenyl group, naphthalene group, and anthracene group; and/or (ii) a natural lipid which is selected from phosphatidyl choline and phosphatidyl serine; and in that the apparatus comprises either (a) a means of measuring the resulting changes in membrane potential and/or membrane resistance due to the adsorbtion of the bitter or odor substances, or (b) in the case where the film is cast on an electrode of a crystal oscillator or a piezoelectric crystal, the apparatus also comprises means for detecting decrease in frequency of the crystal oscillator or piezoelectric crystal due to adsorption of the bitter or odor substances. Apparatus according to claim 2 wherein the means of measuring the resulting changes due to the adsorption of the bitter or odor substances in membrane potential and/or membrane resistance comprises a means of positioning the immobilized bilayer film between an inner aqueous phase free of the bitter or odor substances and an outer aqueous phase containing the bitter or odor substances, and a galvanostat - having measuring probes inserted into the inner and outer aqueous phases respectively.

Inventors: OKAHATA YOSHIO, OKAHATA, YOSHIO
Applicants: SOGO PHARM, SOGO PHARMACEUTICAL COMPANY LIMITED
Application year: 1988
Main classification: G01N27
IPC codes: G01N27, H03H9, G01N5