Butenoic acid amides, their salts, pharmaceutical compositions containing them and process for preparing same

Abstract: Compounds of the general formula (I), wherein R is hydrogen, halogen, C₁₋₄alkyl, C₁₋₄alkoxy or C₁₋₄acylamino group; R₁ is hydrogen or a carboxyl-protective group; and R₂ represents a -(CH₂) n -CO₂R₅ group, wherein n is 1, 2, 3 or 4; and R₅ is hydrogen or a carboxyl-protective group; or R₂ is a -(CH₂) n -NH₂ group, wherein n is 1, 2, 3 or 4 in the E and/or Z configuration, as well as their salts, show a cytoprotective effect and promote the healing of stomach ulcers. Their toxicity is low.

Description: This invention relates to novel 4-oxo-4-(substituted phenyl)butenoic acid amides of E and/or Z configuration as well as their salts, having a cytoprotective action. Several compounds, related to these compounds in both their type of activity and chemical structure, have been described in British patent specification No. 2,096,999. One of the most effective compounds claimed in this cited patent specification was found to be 4-oxo-4-(3,4,5-trimethoxyphenyl)butenoic acid prepared by dehydrating the product obtained from the aldol condensation of 3,4,5-trimethoxyacetophenone with glyoxylic acid. The yield of the aldol reaction amounted to 28%, that of the dehydration to 71%, which means a total yield of only 20%. An object of the present invention is to provide therapeutically useful compounds which can be prepared in a good yield from simple, commercially available starting substances. Thus, the present invention provide 4-oxo-4-(substituted phenyl)butenoic acid amides of the general formula (I) wherein R is hydrogen, halogen, C₁₋₄alkyl, C₁₋₄alkoxy or C₁₋₄acylamino group; R₁ is hydrogen or a carboxyl-protective group; and R₂ represents a -(CH₂) n -CO₂R₅ group, wherein n is 1, 2, 3 or 4; and R₅ is hydrogen or a carboxyl-protective group; or R₂ represents a -(CH₂) n -NH₂ group, wherein n is 1, 2, 3 or 4, the compounds having the E and/or Z configuration, and individual optical isomers or mixtures thereof and salts thereof. The compounds of the general formula (I) contain a double bond and so they can exist either as the E or Z geometrical isomer. According to an other aspect of the invention, there is provided a process for the preparation of 4-oxo-4-(substituted phenyl)butenoic acid amides of the general formula (I), R is hydrogen, halogen, C₁₋₄alkyl, C₁₋₄alkoxy or C₁₋₄acylamino group; R₁ is hydrogen or a carboxyl-protective group; and R₂ represents a -(CH₂) n -CO₂R₅ group, wherein n is 1, 2, 3 or 4; and R₅ stands for hydrogen or a carboxyl-protective group; or R₂ represents a -(CH₂) n -NH₂ group, wherein n is 1, 2, 3 or 4, the compounds having the E and/or Z configuration, as well as salts thereof, which comprises reacting a compound of the general formula (II), wherein R₃ is a carboxyl-protective group; and R₄ is a -(CH₂) n -CO₂R₆ group, wherein n is 1, 2, 3 or 4 and R₆ is a carboxyl-protective group; or R₄ represents a -(CH₂) n -NHR₇ group, wherein n is 1, 2, 3 or 4 and R₇ is an amino-protective group, or an acid addition salt thereof with a substituted butenoic acid of the general formula (III), wherein R is as defined above, or with a carboxyl group-activated derivative thereof, followed, if desired, by removal of any protecting group present and/or, if desired, changing the geometrical configuration about the double bond in any compound of formula (I) obtained, and formation of a salt by reaction with a suitable acid or base. If desired, the compounds of the general formula (I) containing a free carboxyl group can be converted to pharmaceutically acceptable salts with inorganic or organic bases; or, when R₂ in the general formula (I) is a -(CH₂) n -NH₂ group, a pharmaceutical acid addition salt thereof may also be prepared. The compounds of the general formula (I) are effective cytoprotective substances. The starting materials are commercially available or can be prepared by methods described in the literature. Thus, the preparation of 4-oxo-4-phenyl-2(E)-butenoic acid was described in Org. Synth. Coll. Vol. 3 , page 109 (1955); 4-(4-methylphenyl)-4-oxo-2(E)-butenoic acid was prepared according to Pechman [(Berichte 15 , 888 (1982)] whereas 4-(4-methoxyphenyl)-4-oxo-2(E)-butenoic acid was synthetized according to Papa et al. [J. Am. Chem. Soc. 70 , 3356 (1948)]. The amino esters of the general formula (II) were prepared as described in: Houben-Weyl: "Methoden der organischen Chemie", Vol. 15/1 , pp. 316-340, G. Thieme Verlag, Stuttgart (1974). It has been found in the course of our investigations that the reaction of the starting substances could be carried out in a yield of 40 to 70% under the usual conditions. In a preferred process according to the present invention, a substituted butenoic acid of the general formula (II), or a carboxylic group-activated derivative thereof, which may have an E or Z configuration, is reacted with an amino acid derivative of the general formula (II) in an inert organic solvent. Activated derivatives of the compounds of general formula (III) are their esters, acid halides or acid anhydrides or a derivative formed with an activating (coupling) reagent, each of which can be prepared in a manner known per se . Carbodiimides such as N,N-dicyclohexylcarbodiimide may preferably be used as activating reagents. Other activating reagents have been described in a monograph by M. Bodánszky: "Principles of Peptide Synthesis", Springer Verlag, Berlin, Heidelberg, New York and Tokyo (1984). The compounds of the general formula (II) have to be reacted in a carboxyl group protected form. Straight or branched chain C₁₋₄alkyl groups, preferably a tert.-butyl, diphenylmethyl, trimehylbenzyl or phthalimidomethyl group [R. W. Roeske: "The Pepides", Vol. 3 ., page 101 (1981) as well as T. W. Green "Protective Groups in Organic Synthesis", Ed. John Wiley, New York, Chichester, Brisbane, Toronto and Singapore (1981)] may be used for protection. When it is desired to prepare compounds of the general formula (I), wherein R₂ is a -(CH₂) n -NH₂ group, then the amino group of the corresponding compound of general formula (II) has also to be protected. All protective groups used in the peptide chemistry, such as the tert.-butoxy-carbonyl group [R. Schwyzer and W. Rittel: Helv. Chim. Acta 44 , 159 (1961)] are useful for this purpose. These reactions are carried out in an inert organic solvent, preferably dichloromethane at a temperature between -10 °C and +20 °C. The compounds of formula (II) may be used in the form of their individual optical isomers or mixtures thereof. If desired, the E and Z isomers of the compounds of general formula (I) can be converted into each other: e.g. a compound of E configuration can be converted to the product of Z configuration by UV light, preferably in the presence of an inert organic solvent. The compounds of the general formula (I) containing hydrogen as R₁ or R₅, respectively are prepared by removing the group different from hydrogen (suitably the tert.-butyl group), which is bound to the carboxyl group in question, in a known way e.g. by using trifluoroacetic acid. In the course of our pharmacological study it has been found that, when used even in a low dose, the compounds of the general formula (I) show a cytoprotective action (with an oral ED₅₀ value of 2 to 5 mg/kg). This could be demonstrated by using the method of A. Robert [Gastroenterology 77 , 761-767 (1979)] as follows. Starved rats were given absolute ethanol containing concentrated hydrochloric acid which induced longitudinal bleedings in the glandular part of the stomach within a short time. This damaging effect is prevented by cytoprotective substances. The oral ED₅₀ values of some compounds according to the invention, which were examined in the acid-ethanol test, are shown in Table 1. The compounds investigated were as follows: A: diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(S)-aspartate; B: diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(RS)-aspartate; and C: diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(R)-aspartate. Oral ED₅₀ values of some compounds according to the invention in the acid-ethanol test Compound studied Cytoprotective effect ED₅₀ (mg/kg p.o.) A (S) 3.8 B (RS) 2.6 C (R) 1.7 The compound C is not toxic in an oral dose of 1200 mg/kg. An investigation of the healing effect if the compounds, on chronic stomach ulcers induced by acetic acid was carried out as follows. To examine the healing-promoting effect of the compounds, a widely used method, the so-called acetic acid-induced chronic stomach ulcer model, was employed [ Takagi et. al.: Jap. J. Pharmacol. 19 , 418-426 (1969)] The abdominal wall of anaesthetized rats was opened and 50 /µl of a 20% acetic acid solution were injected into the stomach wall, then the abdominal wall of the animal was sutured. The treatment by the test compounds was started on day 5 following the surgical operation and continued till the day 14 by using the daily doses shown in Table 2. Then, the animals were killed and their stomach examined. The extent of ulceration was assessed by measuring the diameter of the ulcer, wherefrom the side of the damaged area could be calculated. For characterizing the degree of the cicatrization, the change in the ulcer index measured in the treated animals was related to the values measured in the untreated (control) animals and expressed as percentage values. The results of these investigations are summarized in Table 2. Effect of some reference drugs on promoting healing of chronic stomach ulcers induced by acetic acid Drug Dose mg/kg/day p.o. Change in the ulcer index related to the control Cimetidine 50 x 10 17 % ↑ Pirenzepine 12 x 10 13 % ↑ Sucralfate 500 x 10 55 % ↑ Cetraxate 150 x 10 49 % ↑ MMSC 50 x 10 59 % ↑ Spizofuron 50 x 10 11 % ↑ ↑ : Promotion of healing ↓ : Aggravation of the ulcer It can be seen from Table 1 that the R isomer of diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-aspartate is the most effective in the cytoprotective test. It is clear from Table 2 that healing of the ulcers was significantly (by 57%) accelerated by even a 3 mg/kg oral dose of the R isomer whereas healing was not prompted by even a 40 mg/kg oral dose of the S isomer. It can be seen that highly significant differences exist between the healing-promoting effects of the R, the RS and the S isomers, and the R-isomers of the compounds of the invention are to be preferred. From Table 3 it appears that the healing-promoting effect of the R isomer highly exceeds that of the reference drugs which are at present being used in human therapy or are under clinical trials. The toxicity values of the substances according to the invention are very favourable since no toxic symptoms were observed even after the single oral administration of 1500 mg/kg. The invention is illustrated in detail by the following non-limiting Examples. Example 1 Preparation of diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(S)-aspartate 10.3 g (0.05 mol) of dicyclohexylcarbodiimide dissolved in 20 ml of anhydrous dichloromethane are dropped to the solution containing 8.8g (0.05 mol) of 4-oxo-4-phenyl-2(E)-butenoic acid in 80 ml of anhydrous dichloromethane at a temperature between -5 °C and -10 °C. After stirring for 15 minutes,11.3 g (0.05 mol) of diethyl (S)-aspartate hydrochloride dissolved in 30 ml of anhydrous dichloromethane, then 5.05 g (0.05 mol) of N-methylmorpholine dissolved in 20 ml of anhydrous dichloromethane are added dropwise to the reaction mixture. After stopping the cooling, the mixture is stirred for 4 hours, the solid precipitate is filtered and washed with dichloromethane. The filtrate is successively extracted twice with 50 ml of 5% sodium hydrogen carbonate solution each, 50 ml of water, twice with 50 ml of 1N hydrochloric acid each and finally with 50 ml of water, then dried over anhydrous magnesium sulfate and evaporated. After recrystallizing the residue from ether, a yield of 8.5 g (49%) of the title compound is obtained, m.p.: 91-93 °C. (After recrystallizing this substance from a 2.5:1 mixture of cyclohexane and benzene 5.8 g of product are obtained, m.p.: 91-92 °C, [ α ]²⁵ -20.7° (c=2, ethanol). Analysis: Calculated for C₁₈H₂₁NO₆: C 62.24; H 6.09; N 4.03%; found: C 62.22; H 6.34; N 3.98%. Example 2 Preparation of diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(R,S)-aspartate The process described in Example 1 is followed, except that 11.3 g (0.05 mol) of diethyl (R,S)-aspartate are used as starting substance to give 7.11 g (41%) of the title compound, m.p.: 84-86 °C. Analysis: Calculated for C₁₈H₂₁NO₆: C 62.24; H 6.09; N 4.03%; found: C 62.25; H 6.40; N 4.01%. Example 3 Preparation of diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl] -(S)-glutamate The process described in Example 1 is followed, except that 11,97 g (0.05 mol) of diethyl (S)-glutamate are used as starting substance to give 7.05 g (39%) of the title compound, m.p.: 83-85 °C, [ α ] 25 / D -30.95° (c=2, ethanol). Analysis: Calculated for C₁₉H₂₃NO₆: C 63.15; H 6.41; N 3.87%; found: C 63.04; H 6.43; N 3.76%. Example 4 Preparation of ethyl N α -[4-oxo-4-phenyl-2(E)-butenoyl]-(S)-lysinate hydrochloride Step a) Preparation of ethyl N α -[4-oxo-4-phenyl-2(E)-butenoyl]-Nε-(tert.-butoxycarbonyl)-(S)-lysinate A solution containing 10.3 g (0.05 mol) of dicyclohexylcarbodiimide in 20 ml of anhydrous dichloromethane is added dropwise to the solution of 8.8 g (0.05 mol) of 4-oxo-4-phenyl-2(E)-butenoic acid in 80 ml of anhydrous dichoromethane at -10 °C. After stirring for 15 minutes, 15 g (0.05 mol) of ethyl Nε-(tert.-butoxycarbonyl)-(S)-lysinate hydrochloride dissolved in 120 ml of anhydrous dichloromehane, then 5.05 g (0.05 mol) of N-methylmorpholine dissolved in 10 ml of anhydrous dichloromethane are added dropwise to the reaction mixture. After stirring for 4 hours, the precipitated dicyclohexylurea is filtered and washed with dichloromethane. The filtrate is successively extracted twice with 50 ml of 5% sodium hydrogen carbonate solution each, with water, twice with 50 ml of 1N hydrochloric acid each, finally with 50 ml of water and dried over anhydrous magnesium sulfate. After evaporating the solvent under reduced pressure, the oily residue is crystallized from a mixture of benzene and petroleum ether (b.p. 70 °C) to give 15.8 g (73%) of the title product of Step a), m.p.: 91-94 °C. Step b) Preparation of ethyl Nα-[4-oxo-4-phenyl-2(E)-butenoyl]-(S)-lysinate hydrochloride 12 g (0.027 mol) of the product obtained in Step a) are stirred for 2 hours with 80 ml of trifluoroacetic acid cooled to 0 °C, then the trifluoroacetic acid is evaporated under reduced pressure. The residue is dissolved in 30 ml of acetonitrile and crystallized by adding 100 ml of diethyl ether to give 9,98 g (83%) of the trifluoroacetate salt, m.p.: 119-121 °C. 4.46 g (0.01 mol) of this trifluoroacetate salt are dissolved in 40 ml of acetonitrile, 2.6 ml of a 6N dioxanic hydrogen chloride solution are added and crystallized by adding 100 ml of diethyl ether under cooling to give 2.95 g (80%) of the title compound of Step b), m.p.: 114-118 °C, [ α ] 25 / D -31.84° (c=2, ethanol). Analysis: Calculated for C₁₈H₂₄N₂O₄.HCl: C 58.62; H 6.83; N 7.59; Cl 9.61%; found: C 58.57; H 6.94; N 7.54; Cl 9.65% Example 5 Preparation of N-[4-oxo-4-phenyl-2(E)-butenoyl]-(S)-aspartic acid A suspension containing 6.71 g (0.02 mol) of di(tert.-butyl) (S)-aspartate oxalate in 25 ml of dichloromethane is extracted with a solution containing 4.14 g of potassium carbonate in 20 ml of water. The aqueous phase is extracted three times, each time with 20 ml of dichloromethane, dried over anhydrous magnesium sulfate and evaporated to a volume of 50 ml. After adding 3.52 g (0.02 mol) of 4-oxo-4-phenyl-2(E)-butenoic acid, the solution is cooled to 0 °C and 4.12 g (mol) of dicyclohexylcarbodiimide dissolved in 10 ml of abs. dichloromethane is added dropwise to the reaction mixture at the same temperature. After stirring the mixture at 0 °C for 1 hour and then at room temperature for 1 hour, the dicyclohexylurea is filtered and washed with dichloromethane. The filtrate is successively extracted twice with 20 ml of 0.1N hydrochloric acid, 50 ml of water, twice with 20 ml of 5% sodium hydrogen carbonate solution and finally with 50 ml of water. After drying over anhydrous magnesium sulfate and evaporating the solvent, the oily residue is dissolved in 40 ml of an 1:1 mixture of dichloromethane and trifluoroacetic acid and let to stand at room temperature for 20 hours. After evaporating the solvent, the residue is crystallized from diethyl ether to give 2.34 g (40 %) of the title compound, m.p.: 154-156 °C, [ α ] 20 / D -7.29° (c=2, methanol). Preparation of the magnesium salt of the title compound: 2 g (6.8 mmol) of N-[4-oxo-4-phenyl-2(E)-butenoyl]-(S)-aspartic acid are dissolved in the solution of 0.76 g (13.6 mmol) of potassium hydroxide in 5 ml of distilled water and 1.51 g (6.8 mmol) of solid magnesium perchlorate are added. The precipitated potassium perchlorate is filtered, the filtrate is evaporated and after adding acetonitrile, the product obtained is filtered to give 1.81 g (85%) of the magnesium salt of the title compound, m.p.: > 360 °C, [ α ] 20 / D +23.9° (c=2, methanol/water = 4:1). Example 6 Preparation of diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(R)-aspartate A solution containing 21.6 g (0.105 mol) of dicyclohexylcarbodiimide in 40 ml of anhydrous dichloromethane is added dropwise to the solution of 17.6 g (0.1 mol) of 4-oxo-4-phenyl-2(E)-butenoic acid in 100 ml of anhydrous dichloromethane at 0 °C. After stirring for 30 minutes, 22.55 g (0.1 mol) of diethyl (R)-aspartate hydrochloride dissolved in 100 ml of dichloromethane is added and then 10.1 g (0.1 mol) of N-methylmorpholine dissolved in 20 ml of anhydrous dichloromethane is added. The reaction mixture is stirred under cooling by ice-water for 3 hours and then at room temperature for 15 hours. The precipitated solid dicyclohexylurea (DCU) is filtered and the filtrate is successively extracted with 5 % sodium hydrogen carbonate solution, water, 1N hydrochloric acid and finally with water. After drying over anhydrous magnesium sulfate and filtering and drying agent, the solvent is evaporated and the residue is crystallized from a 10:1 mixture of cyclohexane and benzene. 24.7 g (71%) of the product are obtained. After recrystallization from diisopropyl ether, 19.4 g (56%) of the title compound are obtained, m.p.: 88-89 °C, [ α ] 25 / D +12.2 (c=2, ethanol).

Claims: Claims for the following Contracting States : AT, BE, FR, DE, GB, IT, NL, SE, CH, LI 4-Oxo-4-(substituted phenyl)butenoic acid amides of the general formula (I), wherein R is hydrogen, halogen, C₁₋₄alkyl, C₁₋₄alkoxy or C₁₋₄acylamino group; R₁ is hydrogen or a carboxyl-protective group; and R₂ represents a -(CH₂) n -CO₂R₅ group, wherein n is 1, 2, 3 or 4; and R₅ is hydrogen or a carboxyl-protective group; or R₂ represents a -(CH₂) n -NH₂ group, wherein n is 1, 2, 3 or 4, the compounds having the E and/or Z configuration, and individual optical isomers or mixtures thereof and salts thereof. Diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(R)-aspartate. A pharmaceutical composition which comprises as active ingredient a 4-oxo-4-(substituted phenyl)butenoic acid amide of the general formula (I), as defined in claim 1 or a salt thereof in admixture with a pharmaceutically acceptable carrier, diluent or excipient. A process for the preparation of 4-oxo-4-(substituted phenyl)butenoic acid amides of the general formula (I), wherein R is hydrogen, halogen, C₁₋₄alkyl, C₁₋₄alkoxy or C₁₋₄acylamino group; R₁ is hydrogen or a carboxyl-protective group; and R₂ represents a (CH₂)n-CO₂R₅ group, wherein n is 1, 2, 3 or 4; and R₅ stands for hydrogen or a carboxyl-protective group; or R₂ represents a -(CH₂)n-NH₂ group, wherein n is 1, 2, 3 or 4, the compounds having the E and/or Z configuration as well as salts thereof, which comprises reacting a compound of the general formula (II), wherein R₃ is a carboxyl-protective group; and R₄ is a -(CH₂) n -CO₂R₆ group, wherein n is 1, 2, 3 or 4 and R₆ is a carboxyl-protective group; or R₄ represents a -(CH₂) n -NHR₇ group, wherein n is 1, 2, 3 or 4 and R₇ is an amino-protective group, or an acid addition salt thereof with a substituted butenoic acid of the general formula (III), wherein R is as defined above, or with a carboxyl group-activated derivative thereof, followed, if desired, by removal of any protecting group present and/or, if desired, changing the geometrical configuration about the double bond in any compound of formula (I) obtained, and formation of a salt by reaction with a suitable acid or base. A process as claimed in claim 4, which comprises using a substituted butenoic acid of the general formula (III), wherein R is as defined in claim 4, in a form activated by a carbodiimide, preferably by dicyclohexylcarbodiimide. A process as claimed in claim 4, which comprises using a substituted butenoic acid of the general formula (III), wherein R is as defined in claim 4, in the form of its ester, acid halide or acid anhydride as activated derivatives. A process as claimed in any of the claims 4 to 6, which comprises changing the configuration about the double bond of the compounds of general formula (I) by using UV light. A process for the preparation of pharmaceutical composition, which comprises bringing into admixture a 4-oxo-4-(substituted phenyl)butenoic acid amide of the general formula (I) as defined in claim 4 or a pharmaceutically acceptable salt thereof, with a pharmaceutically acceptable carrier, diluent or excipient. Use of 4-oxo-(substituted phenyl)butenoic acid amides of the general formula (I) as defined in claim 4, the compound having the E and/or Z configuration, individual optical isomers, mixtures thereof and salts thereof in the preparation of a medicament for use as a cytoprotective agent, and in the promotion of tissue repair. Claims for the following Contracting States : ES, GR A process for the preparation of 4-oxo-4-(substituted phenyl)butenoic acid amides of the general formula (I), wherein R is hydrogen, halogen, C₁₋₄alkyl, C₁₋₄alkoxy or C₁₋₄acylamino group; R₁ is hydrogen or a carboxyl-protective group; and R₂ represents a -(CH₂) n -CO₂R₅ group, wherein n is 1, 2, 3 or 4; and R₅ is hydrogen or a carboxyl-protective group; or R₂ represents a -(CH₂) n -NH₂ group, wherein n is 1, 2, 3 or 4, the compounds having the E and/or Z configuration as well as individual optical isomers mixtures thereof and salts thereof, which comprises reacting a compound of the general formula (II), wherein R₃ is a carboxyl-protective group; and R₄ is a -(CH₂) n -CO₂R₆ group, wherein n is 1, 2, 3 or 4 and R₆ is a carboxyl-protective group; or R₄ represents a -(CH₂) n -NHR₇ group, wherein n is 1, 2, 3 or 4 and R₇ is an amino-protective group, or an acid addition salt thereof with a substituted butenoic acid of the general formula (III), wherein R is as definedabove, or with a carboxyl group-activated derivative thereof, followed, if desired, by removal of any protecting group present and/or, if desired, changing the geometrical configuration about the double bond in any compound of formula (I) obtained, and formation of a salt by reaction with a suitable acid or base. A process as claimed in claim 1, which comprises using a substituted butenoic acid of the general formula (III), wherein R is as defined in claim 1, in a form activated by a carbodiimide, preferably by dicyclohexylcarbodiimide. A process as claimed in claim 1, which comprises using a substituted butenoic acid of the general formula (III), wherein R is as defined in claim 1, in the form of its ester, acid halide or acid anhydride as activated derivatives. A process as claimed in any of the claims 1 to 3, which comprises changing the configuration about the double bond of the compounds of general formula (I) by using UV light. A process as claimed in any one of the preceding claims wherein the compound of formula (II) is used in the form of one of its optical isomers, or as mixtures thereof. A process as claimed in claim 1 for the preparation of diethyl N-[4-oxo-4-phenyl-2(E)-butenoyl]-(R)-aspartate. A process for the preparation of pharmaceutical composition, which comprises bringing into admixture a 4-oxo-4-(substituted phenyl)butenoic acid amide of the general formula (I) as defined in claim 1 or a pharmaceutically acceptable salt thereof, with a pharmaceutically acceptable carrier, diluent or excipient. Use of 4-oxo-(substituted phenyl)butenoic acid amides of the general formula (I) as defined in claim 1, the compound having the E and/or Z configuration, individual optical isomers, mixtures thereof and salts thereof in the preparation of a medicament for use as a cytoprotective agent, and in the promotion of tissue repair.

Inventors: DOBAY LASZLO, EZER ELEMER, FISCHER JANOS, FODOR TAMAS, MATUZ JUDIT, SAGHY KATALIN, STEFKO BELA, SZPORNY LASZLO, DOBAY, LASZLO, EZER, ELEMER, FISCHER, JANOS, FODOR, TAMAS, MATUZ, JUDIT, SAGHY, KATALIN, STEFKO, BELA, SZPORNY, LASZLO
Applicants: RICHTER GEDEON VEGYESZET, RICHTER GEDEON VEGYESZETI GYAR R.T.
Application year: 1988
Main classification: C07C233
IPC codes: C07C231, C07C233, A61P43, C07C235, A61P1, A61K31, C07C67