PHARMACEUTICAL FORMULATION ENSURING THE TRANSDERMAL ABSORPTION OF THE ACTIVE INGREDIENT AND PROCESS FOR PREPARING SAME

Abstract: The invention relates to a multilayer pharmaceutical formulation containing layers with various active ingredient content, a water-impermeable layer applied thereon and an adhesive layer ensuring the fixation to the body surface of the pharmaceutical formulation, which comprises: a silicone rubber layer directly contacting with the skin surface and ensuring the zero order kinetics of the dissolution of the active ingredient; as well as a multilaminated silicone rubber matrix comprising one or more layer(s) with various active ingredient content.

Description: PHARMACEUTICAL FORMULATION ENSURING THE TRANSDERMAL ABSORPTION OF THE ACTIVE INGREDIENT AND PROCESS FOR PREPARING SAME This invention relates to a multilayer pharmaceutical formulation which, when put onto the skin, ensures the protracted (suitably lasting about 18 to 24 hours) absorption with so called zero order kinetics of the incorporated active ingredient. According to an other aspect ' bf the invention, there is provided a process for the preparation of these multilayer pharmaceutical formulations. There are known pharmaceutical formulations in the prior art which ensure the transdermal absorption of the incorporated active ingredient content. The polymeric matrix of a πitro- glycerin-coπtaining composition commercialized by Key Pharmaceuticals Inc. under the trade mark Nitro-Our contains 2 to 0% of glycerol, 2 to 15% of polyviπyl alcohol and 2 to 10% of a water-soluble polymer, e.g. PVP (see the United States patent specification No. 4,466,953). This polymeric matrix ensures the suitable dissolution kinetics of the active ingredient. The Transder compositions produced by Alza Co. are pharmaceutical formulations consisting of four layers which contain the active ingredient in an admixture with an A 4325-741 MR ointment based on a silicone; the dissolution of the active ingredient is regulated by a non-porous membrane prepared from ethylene/viπyl acetate. For ensuring the initial dose, the adhesive layer of this composition also contains 8% 5 of active ingredient. The compositions produced by Searle Co., e.g. Nitrodisc (R, are prepared in such a way that the active ingredient is suspended in the solution of a water-soluble polymer and the suspension obtained is mixed into a silicone polymer; there- 10 after, the cross-linking is carried out and finally, a specific membrane layer is also applied in order to regulate the dis¬ solution of the active ingredient (see the United States patent specification No. 3,946,106). The structure of the Nitroderm-TTS compositions of L5 Ciba-Geigy AG is practically identical to that of the Transderm compositions. Summing up, the structure of the formulations known up to the present are as follows. 1) Matrix system 1. diffusion-regulating polymeric 20 (Figure 1) matrix (gel, ointment); 2. solid active ingredient; 3. Impermeable polymeric layer. /_~Example: Nitro-Dur (Key Pharm._)7 2) Layered system 1. protective foil; 25 (Figure 2) 2. ointment (gel) containing active ingredient; 3. dissolution-regulating membrane; 4. adhesive layer containing the active ingredient. / " Example: Traπsderm-Nitro 3) Microsealed system 1. dissolution-regulating (Figure 3) polymeric membrane; 2. aqueous polymeric drops containing an- active ingredient suspension; 3. polymeric matrix (based on a silicone) ; 4.. impermeable polymeric layer. (R /_ Example: Nitrodisc ^ ( Sear le Y The pharmaceutical formulation according to the inveπ- tion is a bilayer or multilayer system with a structure illustrated in Figure 4, wherein A is a silicone rubber layer ensuring the dissolution with zero order kinetics of the active ingredient; B is a multilaminated silicone rubber matrix involving layers (n = 1 to 6) with various active ingredient content; C is an aluminum foil or other suitable, water-imperme¬ able foil layer; and D is an adhesive layer providing the fixation of the formulation to the body surface. The layers A and B of the pharmaceutical formulation according to the invention are prepared by a catalytic (protolytic) polycondensation or polyaddition process, using one- or two-component silicone rubber basic material. The lay 1 ers A and Bn mentioned above can be prepared in various thicknesses (0.1 to 3 mm): by the catalytic polycondensation of various alkylpolysiloxane-ct,^-diols (suitably met ' hyl- polysiloxaπe-o , CO-diols having a low molecular weight (M = 500 to 100,000) and/or higher molecular weight (M = 100,000 to 20,000,000); or by the catalytic addition of polysiloxaπe polymers containing various alkyl ^ c n H 2π + l^' aryl ^ c g H 5' C 6 HcCH 2 ), alkenyl (CH 2 =CH-) or -H groups and bearing as terminal " moiety a reactive group (OH, H, vinyl and the like) or a mono- functional group /_ " (CH 3 ) 3 SiO, (CH 3 ) 2 CH 2 -CHSiO- and the like/. The lay 1 ers A and Bn (wherein n is suitably J 1 to 5) are silicone rubber matrices one layered on the other with various active ingredient content, e.g. 0 to 20%, with the proviso that at least one layer contains active ingredient. Any catalyst providing cross-linking through condensation (suitably the T-5 product of Wacker Co., FRG) may be used as poly¬ condensation catalyst in an amount of 0.01 to 15%, suitably 5 to 8%. Various noble metal salts or noble metal complexes may e used as polyaddition catalysts in an amount of 1 to 100 ppm. This process can be carried out at a wide temperature range, from 15 to 140 °C, suitably between 20 °C and 40 °C, depend¬ ing on the properties of the incorporated active ingredient. This process is useful for both the batch or continuous production of the pharmaceutical formulation according to the invention. According to a preferred embodiment of the invention, a pharmaceutical formulation is prepared which is a multi- laminated system built up from silicone rubber layers of various layer thickness and with various active ingredient content. This system is provided -with a suitable water- -impermeable layer, conveniently with an aluminum foil layer, over the silicone rubber layers and with an adhesive layer ensuring the sticking to the skin surface. The first layer of 0.1 to 3 mm in thickness of the pharmaceutical formulation, which adheres to the skin surface- -and contains no active ingredient (signed by "A" in Figure 1), is closely built together with the subsequent layers of various active ingredient content and thus, the zero order dissolution kinetics of the- active ingredient is provided by the whole laminated system. In the compositions according to the invention quite different drugs, e.g. cardiovascular, spasmolytic, anorectogenic aπtidiabetic agents as well as drugs affecting the hormone system may be used as active ingredients. According to the invention, the multilaminated system is formed by polymerizing the individual layers one by one on the surface of each other and one after the other in time. Depending from the active ingredient of the system, the layer A can be produced by polycondensation in various layer thickness, e.g. 0.1 to 3 mm, at various temperatures, e.g. between 15 °C and 90 °C, suitably at 25 to 60 °C, of a mixture prepared from e.g. various polydimethylsiloxaπe- ol,60- -diols (hereinafter abbreviated: PDSD), suitably from the Silo- rol R-l, R-5 and R-30 products of Fiπomvegyszer Szovetkezet Co., Budapest, Hungary, or from the mixtures thereof, by using various (polycondensation involving) cross-linking catalysts (hereinafter abbreviated: CLC) in an amount of 0.1 to 15%, suitably 5 to 10%. A trifunctioπal methylsilaπe derivative containing Sl-N- and/or Si-0 bond, conveniently methyltricyclohexyl-amiπosilaπe, can be used as catalyst or main component; a tetraalkoxysilane, suitably tetraethoxy- silane, tetra ethoxysilane or the like are used as main- -compoπeπts; and a catalyst mixture containing a dialkyltiπ salt of a long-chain fatty- acid, suitably dibαtyltiπ laurate is employed as initiator. In the case of polysiloxane polymers containing alkyl ( c π H 2n + l' '' aryl ^ C 6 H 5' C £ H 5 CH 2^' alkeπvl (CH 2 =CH-) or -H groups and bearing a reactive group (OH, H, vinyl and the like) or a monofuπctioπal grouping /_ ~ (CH- j ),SiO, (CH,) 2 CH 2 ~CHSiO- or the like/ as a terminal moiety (herein¬ after abbreviated: PSP) the process is carried out in such a way that the layer thickness of the layer A, resulting from the polyaddition under the effect αf noble metal salts or noble metal complexes (hereinafter abbreviated: NM type catalysts) used as catalysts and being present in an amount of 1 to 100 ppm, will be 0.1 to 3 mm and the polyaddition process is performed at a temperature between 15 °C and 140 °C. The first layer B-, containing an active ingredient is applied onto the layer A thus prepared. The layer B, can suitably be prepared by polymerizing at various temperatures, at 15 to 140 °c, suitably at 15 to 50 °C, a homogenised mixture prepared from the active ingredient as such or from a dilution thereof with a solid or liquid diluent, e.g. lactose, glucose, Aerosil and the like, and from the PDSD or PSP basic material used in varying amounts, e.g. from 0.5 to 20% suitably from 0.5 to 4%, with a CLC or NM type catalyst. When the layer B-, is formed by polycondensation, the amount of a CLC type catalyst may preferably amount to 0.1 to 15%, suitably 5 to 10%, and the preferred temperature may be 15 to 90 °C, suitably 20 to 50 °C. When using the polyaddition process, the amount of a NM type catalyst may be 1 to 100 ppm and the process may be carried out at a temperature between 15 °C and' 140 C, suitably at 20 to 60 °C. Depending from the nature and ' properties of the active ingredient, the layers B are applied onto the polymerized layers B , by repeating the process described hereiπabove with a successively increasing active ingredient content between 15% and 25%,differing from the active ingredient content of the layer Bπ„-1-, . Onto the last layer B , PDSD or PSP basic material, suitably in a thickness of 0.1 to 1 mm and containing no active ingredient and 0.1 to 15%, suitably 5 to 8%, of a CLC type catalyst or 1 to 100 ppm, suitably 20 ppm, of a NM type catalyst, is applied, and after the attachment of the water- -impermeable layer, suitably aluminum foil, the polymerization is carried out at a temperature between 15 C and 140 C, suitably at 25 to 50 °C. Finally, the adhesive layer provid¬ ing the sticking to the skin of the formulation, is bound to the water-impermeable layer. The formulations according to the invention were investigated in _iπ_ vitro studies involving the determination of the active ingredient release during the time unit. (mg/h), the rate of dissolution of the active ingredient (mg/cm /h) as well as the percentage of dissolution of the total active ingredient content. The dissolution was followed in a Keshary-Chieπ type diffusion cell Λ~c.f. Drug Development Industrial Pharmacy 10 (6), pp. 883-913, 1984 . In these measurements the sample 2 surface was 3.14 cm ; the liquid was an isotonic sodium chloride solution with a volume of 10 ml and with a temperature of 37 C. The volume of the sample was 1 ml. 10 The active ingredient content of the samples was determined by measuring the UV extinction by using a VSU 2-P device; Carl Zeiss Jena, GDR. Nitroglycerin was measured at 207 nm in a layer thickness of 2 mm against an isotonic sodium chloride solution. Isosorbide dinitrate was determined at 220 nm --^ in a layer thickness of 2 mm against distilled water. It can be stated as a conclusion that, under the experimental conditions used, the formulations according to the invention show uniform release of the active ingredient within about 24 hours. 2° The results of the dissolution studies are illustrated in the graphs 1 and 2. The short-time stability of the formulations was also studied. Since nitroglycerin used as active ingredient is a volatile substance, the nitroglycerin content of the πitro- 25 glycerin-lactose trituration should continuously be controlled. The formulations were stored as packaged in aluminum foil at +5 C or +30 C and examined in regular intervals. It was stated that the formulations according to the invention could be stored without any change both at +5 °C and +30 °C as well. The experimental results obtained with the formulation of Example 1 containing nitroglycerin are illustrated in. Tables 1 and 2. Table 1 (Storage at +30 α C) Average dissolution day 1 day 7 day 21 day 60 mg/cm 2 /h 6.63.10 5 7.3.10 "5 6.61.10 "5 6.B2.10 5 Standard deviation of the data 2.29.10 "5 2.7.10 "5 1.47.10 "5 2.09.10 "5 Mean deviation 9.3 .10 "6 1.05.10 "5 6.01.10 "6 8.6 .10 "6 Table 2 ( Storage a t +5 O r C ) day 1 day 7 day 21 day 60 Average dissolution mg/cmVh 8.61.10 J 8.9.10 ° 9.19.10 J 8.8.10 D Standard deviation of the data 2.1 . 10 "5 2.9 .10 "3 2.5 .10 "5 2.08 Mean deviation 8.9 . 10 "6 8.5 .10 "6 1.03.10 "5 8.51 When compared to the formulations of the prior art, the formulation according to the invention shows the follow¬ ing advantages. - The building-up of the formulation makes possible the preparation of any transdermally absorbeable pharmaceutical composition as well as a uniform therapeutic blood level of the active ingredient for a longer period, suitably for 18 to 24 hours. - The silicone rubbers employed as basic materials are cheap and physiologically inert to the living organism and do not go into any interaction with the incorporated active ingredient. - The zero order kinetics of the dissolution of the active ingredient can be ensured by the suitable selection of the basic materials used, their layer thickness and the active ingredient content of the individual layers. - No porous or microporous membranes requiring a particular basic material or a specific construction are needed to regulate the dissolution of the active ingredient. - The adhesion of the individual layers to each other and thus, the ensuring of an appropriate structure can easily be satisfied since the layers are essentially identical from the chemical point of view. The invention is illustrated in detail by the follow- ing non-limiting Examples. Example 1 a) A mixture containing 60% of dimethylpolysiloxane-O , l - -diol with a viscosity of 50,000 mPa.s and 35% of the same material with a viscosity of 1,000 mPa.s is homogenized under vigorous stirring for 2 minutes, then 5% of Wacker T-5 catalyst are added to the polymeric mixture and the stirring is continued for additional 2 minutes. The homogeneous mixture thus obtained is stretched out in a layer thickness of 0.5 mm in a suitable equipment and cross-linked at 40 °C for 3 hours (layer A).- b) A mixture containing 50% of dimethylpolysiloxane- -JL , Co- -diol with a viscosity of 5,000 mPa.s and 25% of the same material with a viscosity of 1,000 mPa.s is homogenized under vigorous stirring for 2 minutes, then 20% of a lactose-tri- turation containing 10% of nitroglycerin are portioπwise added, the mixture is slowly stirred for 5 minutes and then, after adding 5% of Wacker T-5 catalyst, the homogenisation is continued for additional 5 minutes. The mass obtained is stretched out in a layer thickness of 0.5 mm onto the layer A in a suitable equipment and then cross-linked at 40 °C for 3 hours (layer B-,) c) By using the process described under b) and applying as components 35% of dimethylpolysiloxane- oL, £ -diol with a viscosity of 5,000 mPa.s, 20% of the same material with a viscosity of 1,000 mPa.s, 40% of a lactαse-trituratiσn- contaiπiπg 10% of nitroglycerin and 5% of Wacker T-5 catalyst - onto the layers A + B, in a thickness of 1 mm, the layer B 2 is cross-linked at 40 °C for 3 hours. d) • A homogeneous mixture prepared by the process described under b) from 30% of dimethylpolysiloxane- at, Cλ diol with a viscosity of 5,000 mPa.s, 15% of the same material with 1,000 mPa.s as well as from 50% of a lactose-trituration containing 10% of nitroglycerin and 5% of Wacker T-5 catalyst is applied in a thickness of 1 mm onto the surface of the laminated matrix A + B, + B 2 and then cross-linked at 40 °C for 3 hours, giving layer B-r. e) A layer consisting of 95% of dimethylpolysiloxane- <= } - O ,-diol with a viscosity of 1,000 mPa.s and 5% of Wacker T-5 catalyst is applied in a thickness of 0.1 mm onto the layer B- j of the laminated matrix consisting of the layers A + B, + B 2 + B, and then covered by a thin aluminum foil and cross-linked at 40 °C for 3 hours. f) An adhesive textile layer is applied onto the aluminum foil layer which reaches beyond the polymeric metrix and ensures the sticking to the skin of the formulation. Example 2 The layers are built up in the succession of operations described in Example 1, except that:- a) The thickness of the layer A is 1 mm and it contains 50% of dimethylpolysiloxane--Ct -diol with a viscosity of 50,000 mPa.s, 45% of the same with a. viscosity of 5,000 mPa.s as well as 5% of Wacker T-5 catalyst. The polymerization is carried out at 60 C for 90 minutes. b) The thickness of the layer B, is 1 mm and it contains 70% of a dimethylpolysiloxane- o , 60-diol with a viscosity of 50,000 mPa.s and 20% of the same material with a viscosity of 5,000 mPa.s, as well as 5% of Phenobarbital sodium and 5% of Wacker T-5 catalyst. The polymerization is carried out at 40 °C for 3 hours. c) The thickness of the layer B 2 is 1 mm and it contains 70% of dimethylpαlysiloxaπe-o ,CU-diol with a viscosity of 50,000 mPa.s, 17% of the same material with a viscosity of 5,000 mPa.s, 8% of Phenobarbital sodium as well as 5% of Wacker T-5 catalyst. The polymerization is carried out at 40 °C for 3 hours. d) The thickness of the layer B-, is 1 mm and it contains 70% of dimethylpolysiloxane- Q ,6u-diol with a viscosity of 50,000 mPa.s, 13% of the same material with a viscosity of 5,000 mPa.s, 12% of Phenobarbital sodium and 5% of Wacker T-5 catalyst. The polymerization is carried out at 40 C for 3 hours. The layers C and D are prepared as .described under e) and f) of Example 1. Example 3 The layers are built up in- the succession of operations described in Example 1, except that: a) The thickness of the layer A is 1 mm and it contains Wacker 3003/50 type two-component silicone rubber (able for polyaddition) as basic material, the components A and B of which are homogenized in a ratio of 50% : 50%. The poly- erization is carried out at 70 α C for 25 minutes. b) The thickness of the layer B-, is 1 mm and it contains 35% of dimethylpolysiloxane- oC, ->-diol with a viscosity of 50,000 mPa.s, 40% of the same material with a viscosity of 5,000 mPa.s, 20% of a lactose-trituration containing 40% of isosorbide dinitrate as active ingredient as well as 5% of Wacker T-5 catalyst. The polymerization is carried out at 40 °C for 3 hours. c) The thickness of the layer B 2 is 0.5 mm and it contains 25% dimethylpolysiloxane-cC, C^-diol with a viscosity of 50,000 mPa.s, 30% of the same material with a viscosity of 5,000 mPa.s, 40% of a lactose-trituration containing 40% of isosorbide dinitrate as active ingredient as well as 5% of Wacker T-5 catalyst. The polymerization is carried out at 40 °C for 3 hours. d) The thickness of the layer B-, is 0.5 mm and it contains 20% of dimethylpolysiloxane- c with a viscosity of 50,000 mPa.s, 30% of the same material with a viscosity of 5,000 mPa.s, 45% of a lactose-trituration containing 40% of isosorbide dinitrate as active ingredient as well as 5% of Wacker T-5 catalyst. The polymerization is carried out at 40 °C for 3 hours. The layers C and D are prepared as described under e) and f) of Example 1. Example 4 The layers are built up in the succession of operations described in Example 1, except that: a) The thickness of the layer A is 0.25 mm and it contains Wacker 3003/50 type two-component silicone rubber as basic material, the components A and B of which are homogenized in a ratio of 50% : 50%. The polymerization is carried out at 70 C for 30 minutes. b) The layer B, contains Wacker 3003/50 type two-component silicone rubber as basic material, the components A and B of which are homogenized in a ratio of 35 : 35% and then 30% o ~ f pulverized 0-acetylsalicylic acid are added. At the end o.f ttre homogenizatioπ process it is stretched out onto the ' layer A in a layer thickness of 1.5 mm. The polymerization is carried out at 50 °C for 1 hour. c) The thickness of the layer B 2 is 1.5 mm and it contains the components A and B of the Wacker 3003/50 type silicon rubber basic material in a ratio of 30% : 30% and 40% of pulverized 0-acetylsalicylic acid. The polymerization is carried out at 50 °C for 1 hours. The layers C and D are prepared as described under e ) and f) of Example 1. The catalyst (suitably a platinum complex compound) required to the polyaddition processes of Examples 3 and 4 is contained in one of the components of the silicone rubber manufactured industrially, by Wacker.

Claims: C l a i m s 1. Multilayer pharmaceutical formulation containing layers with various active ingredient content, a water- -impermeable layer applied thereon and an adhesive layer ensuring the fixation to the body surface of the pharmaceutical formulation, which compr i s es : a silicone rubber layer directly contacting with the skin surface and .ensuring the zero order kinetics of the dissolution of the active ingredient; as well as a multilaminated silicone rubber matrix comprising one or more layer(s) with various active ingredient content. 2. Process for the preparation of a pharmaceutical formulation useful for the traπsdermal administration with zero order kinetics of active ingredients belonging to various pharmacological groups, which compr i s es building up a system comprising 2 to n layers of a silicone rubber polymer, optionally containing with various additives, wherein the individual layers contain 0 tύ 25% of active ingredient. 3. A process as claimed in claim 2, which compr i s es coating the layer being farthest from the skin surface and containing active ingredient with a water- -impermeable layer, suitably with aluminum foil and covering it with an adhesive layer ensuring the fixation to the skin surface of the pharmaceutical formulation. 4. A process as claimed in claim 2, which comprises carrying out the formation of the individual layers by the polycondensation of alkylpolysiloxane- θt,( - -diols or their mixtures of various ratios, having various viscosity values, suitably a viscosity of 500 to 100,000 mPa.s, by adding an amount of 0.1 to 15% of a catalyst, suitably a Wacker T-5 catalyst, ensuring cross-linking through poly¬ condensation. - 5. A process as claimed in claim 2, which compr ises building up the individual layers by the catalytic addition of polysiloxane polymers or their mixtures containing various alkyl c n H 2n + l^ 5 ar> y^ (preferably C , Hc, C HcCH 2 ) , alkenyl (CH 2 =CH-) or -H groups and bearing as terminal moiety a reactive group (preferably OH group) or a monofunctional group " preferably (CH,) 2 CH 2 -CHSiO- group_7, wherein a noble metal salt or noble metal complex catalyst is present in an amount of 1 to 100 ppm. 6. A process as claimed in any of the claims 2 to 5, 5 which compri ses forming layers of 0.1 to 3 mm in thickness with an active ingredient content of 0 to 25%. 7. A process as claimed in any of the claims 2 to 6, which comprises intro " duciπg to the individual layers the active ingredient as such or in the form of a dilution 0 of the active ingredient with various solid or liquid diluents, suitably with lactose, glucose or Aerosil. 8. A process as claimed in any of the claims 2 to 7, which comprises carrying out the polyaddition or polycondensation process at a temperature between 15 °c and 5 140 °C, suitably between 20 °C and 50 °C.

Inventors: NAGY JOZSEF, SALAMON FERENC, WAGNER OEDOEN, NAGY, JOZSEF, SALAMON, FERENC, WAGNER, OEDOEN
Applicants: BIOGAL GYOGYSZERGYAR
Application year: 1988
Main classification: A61K9
IPC codes: A61F13, A61K9