BISPHOSPHONIC ACID DERIVATIVES AND MEDICINES CONTAINING THE SAME

Abstract: Bisphosphonic acid derivatives of formula (I) and their salts have bone resportion-inhibiting and an anti-arthritis activity:

Description: The present invention provides new bisphosphonic acid derivatives (I′) and pharmaceutically acceptable salts thereof. in which R¹ is a C₁-C₁₀ alkyl group a C₃-C₁₀ cycloalkyl group, a phenylgroup, a C₂-C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅ alkyl) group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups, provided that when R¹ is a methyl, ethyl, isopropyl or tert-butyl group at least one of R², R³, R⁴, R⁵ and R⁶ is not a hydrogen atom. The present invention also provides a bone resorption-inhibitor and an anti-arthritis agent containing, as an active ingredient, a bisphosphonic acid derivative (I) or a pharmaceutically acceptable salt thereof. in which R¹ is a C₁-C₁₀ alkyl group, a C₃-C₁₀ cycloalkyl group, a phenyl group, a C₂-C₁₀ alkenyl group which may be substituted by a phenyl group or a phenyl substituted (C₁-C₅ alkyl) group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is- a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups. Hitherto, various compounds have been synthesized as bisphosphonic acid derivatives, and there may be mentioned JP-A-89293/80 (published unexamined Japanese Patent Application 89293/80) which discloses compounds having an aminomethylene-bisphosphonic acid residue bonded at the 3-position of a substituted isoxazolyl group, like the compounds of the present invention JP-A-89293/80 mentions bisphosphonic acid series compounds of formula: in which R 1a represents an alkyl group or a halogen atom; R 2a represents a hydrogen atom or an alkyl group; and Y represents an oxygen atom or an NH group. However, the substituted isoxazolylaminomethylene-bisphosphonic acid derivatives which are concretely illustrated in the examples of JP-A-89293/80 are those having a methyl, ethyl, isopropyl or tert-butyl group as the alkyl group for the substituent on the isoxazolyl group, and further, only free bisphosphonic acids are illustrated therein. Specifically, only the compounds (I″): in which R 1′ represents a methyl, ethyl, isopropyl or tert-butyl group, are concretely illustrated in JP-A-89293/80. Further, JP-A-89293/80 mentions that the substituted isoxazolylaminomethylene-bisphosphonic acid derivatives can be used as agricultural chemicals, especially as herbicides, but is quite silent on the usability of the said derivatives as medicines. EP-A-186405 disclose: pharmaceutical compounds, for treating abnormal calcium and phosphate metabolism, which are structurally different from those of formula (I′) in that R¹ is always H and the isoxazolyl ring oxygen is replaced by ―CH=H―. Ordinary bones are living tissues which participate in resorption and precipitation of calcium for maintaining a constant inorganic equilibrium in a living body. In growing bones, the inorganic precipitation exceeds the inorganic resorption, but the bone resorption often exceeds the bone precipitation (ossification) in special diseases, inducing hypercalcemia, Paget's disease, etc. Hitherto 1-hydroxyethylidene-1,1-bisphosphonic acid (etidronate), dichloromethylene-bisphosphonic acid (chlodronate), etc. have been used as remedial medicines for diseases caused by bone resorption, but these are inadequate in that their activity is not high and in that they have harmful side effects. Accordingly, satisfactory medicines for the diseases are unknown up to the present. Arthritides are inflammatory diseases of articulations, and the main diseases include rheumatic arthritis and analogous diseases with articular inflammation. Rheumatic arthritis is called rheumatoid arthritis, which is a cryptogenetic chronic polyarthritis where the main lesion resides in the inflammatory lesion in the synovial membrane in the intracapsular layer. Arthritides such as rheumatic arthritis, etc. are progressive diseases which cause articular disorders such as articular deformation, ankylosis, etc. Accordingly, if these progress with no effective remedial treatment, they often cause severe somatic disorders. Various medicines have heretofore been used for the medicinal treatment of these arthritides, including, for example, steroids such as cortisone and other adrenocortical hormones; non-steroid series anti-inflammatory agents such as aspirin, piroxicam, indometacin, etc.; gold agents such as gold thiomalate, etc.; anti-rheumatic agents such as chloroquine preparation, D-penicillamine, etc.; anti-gout agents such as colchicine; and immunosuppressants such as cyclophosphamide, azathioprine, methotrexate, levamisole, etc. However, these medicines have various problems, e.g. they have harmful side-effects which are serious or make prolonged use difficult, or their effect is insufficient, etc. Accordingly, the provision of chemical medicines which are less toxic and which have good preventive and remedial activity against arthritides is strongly desired. The compounds (I′) and salts thereof are new, and we have found, as a result of animal tests, that the compounds (I) and salts thereof unexpectedly have bone resorption-inhibitor activity (e.g. so as to inhibit hypercalcemia caused by bone resorption) as well as anti-arthritic activity. Specifically, the present invention provides the new bisphosphonic acid derivatives (I′) and salts thereof. Additionally, it provides a medicament (e.g. a bone resorption-inhibitor or an anti-arthritic containing, as an active ingredient, bisphosphonic acid derivative (I) or its salt. The compounds (I) and their salts include the bisphosphonic acid derivatives (I′) and (I″) and their salts. In the groups in the general formulae herein a C₁-C₅ alkyl group ("lower alkyl group") is a linear or branched carbon chain. Accordingly, the lower alkyl group includes methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl (amyl), isopentyl, and neopentyl groups etc. The C₁-C₁₀ alkyl group is a linear or branched carbon chain, which includes, in addition to the above-mentioned examples for the lower alkyl group, n-hexyl, n-heptyl, n-octyl, n-nonyl, n-decyl, isoheptyl, 2-ethylbutyl, 2-ethylpentyl, and 4-ethylheptyl groups etc. The C₃-C₁₀ cycloalkyl group includes, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclooctyl, and cyclodecyl groups etc. The C₂-C₁₀ alkenyl group is a linear or branched hydrocarbon group, e.g. a vinyl, alkyl, isopropenyl, 2-butenyl, 2-methyl-2-propenyl, 3-pentenyl, 4-methyl-3-pentenyl, 4-methyl-3-hexenyl, or 4-ethyl-3-hexenyl group, etc. These alkenyl groups may be substituted by a phenyl group. Typical phenylalkenyl groups include styryl, 3-phenyl-2-propenyl, and 4-phenyl-2-butenyl groups etc. The "phenyl substituted (C₁-C₅ alkyl) group which may be substituted by a C₁-C₅ alkoxy group ("lower alkoxy group") includes benzyl, phenethyl, phenylpropyl, and phenylbutyl groups etc. which are unsubstituted or substituted by a methoxy, ethoxy, propoxy, butoxy, pentyloxy, iso-propoxy, iso-butoxy, iso-pentyloxy or tert-pentyloxy group, etc. The said lower alkoxy group may be substituted in any position on the phenyl group. The C₂-C₆ alkanoyl group ("lower alkanoyl group") includes, for example, acetyl, propionyl, and butanoyl groups etc. R¹ is preferably a lower alkyl or a cycloalkyl group, more preferably a methyl, pentyl, or cyclopropyl group, etc. R¹ may be substituted in the 4- or 5-position on the isoxazole ring. The compounds of the present invention include tetraesters in which R³ to R⁶ are all lower alkyl groups as well as monoesters, diesters and triesters in which one to three of R³ to R⁶ is(are) lower alkyl group(s). The free phosphonic acids of the present invention can form the corresponding salts. Accordingly, the active ingredients of the present invention include pharmaceutically acceptable salts of the compounds (I); there may be mentioned salts with inorganic bases, e.g. with alkali metals such as sodium, potassium, etc; with alkaline earth metals such as calcium, magnesium, etc.; with organic bases such as methylamine, ethylamine, dimethylamine, diethylamine, trimethylamine, triethylamine, cyclohexylamine, ethanolamine, diethanolamine, etc.; salts with basic amino acids such as lysine, ornithine, etc; and ammonium salts. Compounds of the present invention can be manufactured in accordance with the following reaction formulae. In these formulae, R¹, R², R³, R⁴, R⁵ and R⁶ have the same significance as above, R 3′ , R 4′ , R 5′ and R 6′ are the same or different C₁-C₅ alkyl groups; R⁷, R⁸, R⁹ and R¹⁰ are the same or different, C₁-C₅ alkyl groups; and X is a halogen atom. First Method: In this method, 3-aminoisoxazole (II), a lower alkyl ortho-formate (III) and a dialkyl phosphite (IV) are blended in stoichiometric amounts or with (IV) in excess and reacted under heating. A reaction solvent is not specifically required. The reaction can be performed at 100 to 200°C, preferably at 150°C or so, for 10 to 60 minutes. For isolating and purifying the reaction product (V), for example, the reaction mixture is purified on a silicagel column with a mixed solvent of methanol-chloroform. In the reaction of the 3-aminoisoxazole (II) and the lower alkyl ortho-formate (III), an iminoether intermediate of formula: may be isolated; this can be further reacted with the dialkyl phosphite (IV) to give the compound (V). For isolation and purification of the reaction product, for example, the reaction mixture is directly purified by silica gel column chromatography; or it can be washed with water in the form of a chloroform solution, and after the solvent is distilled out, the resulting residue can be purified by silica gel column chromatography. For final products in which R² is lower alkanoyl, compound (V) (especially the ester forms) is acylated. For the acylation, compound (V) may be reacted with a stoichiometric or excess amount of acylating agent directly or in a solvent. As the acylating agent can be used acid anhydrides, acid halides, etc. As the reaction solvent can be used benzene, toluene, diglyme, etc. The reaction is desirably carried out under heating. The bisphosphonates can be converted into the corresponding bisphosphonic acids by hydrolysis, e.g. by heating under reflex in concentrated hydrochloric acid, or by treatment with with strong acid or trimethylsilyl halide in a water-free solvent; commercial anhydrous hydrobromic acid in acetic acid can be used directly or appropriately diluted, or trimethylsilyl iodide dissolved in a solvent such as carbon tetrachloride, dimethylformamide, chloroform, toluene, etc. can be used. Hydrolysis may be carried out with cooling or heating. For example, when the ester is hydrolyzed with a trimethylsilyl halide with cooling at -10°C or lower, a partially hydrolyzed product is obtained. When the bisphosphonic acid is to be converted into its salt, it can be treated with a base such as sodium hydroxide, potassium hydroxide, ammonia or organic amines, etc. in conventional manner. Second Method: In this method, a solution of e.g. 1 mol. of phosphorus trihalogenide (VI) and excess, preferably 2 to 10 mol., of trialkyl phosphite (VII) is first reacted, for example, at 40 to 100°C, preferably at 60 to 80°C, for 15 to 30 minutes, and then substituted isoxazole-3-formamide (VIII) is added to the resulting mixture and heated, for example at 40 to 100°C, preferably at 60 to 80°C, for several hours. The progress of the reaction can easily be confirmed by TLC (thin layer chromatography, with developer system of chloroform-methanol). After completion of the reaction, excess trialkyl phosphite can be removed by distillation. Isolation and purification of the product, and optional acylation, hydrolysis and salt formation can be performed as in the first method. Compounds (I) can also be produced by various other methods. For instance, as in JP-A- No. 89293/80, free bisphosphonic acid is first obtained and then esterified or subjected to salt formation. The isolation and purification of the final products can be carried out by conventional chemical treatments which include extraction, crystallization, recrystallization, various chromatography operations, etc. The compounds (I) and their salts provided by the present invention have bone resorption-inhibitory action and also inhibit hypercalcemia caused by bone resorption. Accordingly, the compounds (I) and their salts are useful as a remedy or preventative for hypercalcemia caused by bone resorption, Paget's disease, metastatic osteocarcinoma, osteopsathrosis, sthenic bone resorption following inflammatory arthritides such as chronic rheumatoid arthritis, etc. In addition, they have anti-inflammatory action and sedative and analgesic action. The anti-arthritic action of compounds of the present invention has been demonstrated and confirmed by their preventative and remedial activity against adjuvant-induced arthritis. Adjuvant-induced arthritis is widely utilized as a study model for arthritides in this technical field, as this results in polyarthritis chronica which is analogous to human rheumatic arthritis. Compounds provided by the present invention have been demonstrated and confirmed to have prophylactic and therapeutic actions against adjuvant arthritis. Although it is not clear by what function and mechanism the compounds have anti-arthritic action, it is presumed that they have direct inhibitory action against at least the inflammatory symptoms of arthritides, in view of the reported fact that conventional immunosuppressants and immunomodulators could not be recognized to be effective on already expressed arthritides, while glucocorticosteroids could have anti-arthritic action in both prophylactic and therapeutic experiments for arthritides. Accordingly, the compounds can be applied to all arthritides which cause inflammation. Diseases for which the compounds are efficacious include rheumatic arthritis and arthritides which cause inflammation, for example cryptogenetic polyarthritides such as juvenile rheumatoid arthritis (including Still's disease), tetanic myelitis, psoriatic arthritis, Reiter's syndrome, etc.; rheumatic fever (acute rheumatic arthritis); diseases causing arthritic symptoms, e.g. gouty and metabolic articular diseases such as gout, pseudogout, Wilson's diseases, etc.; diseases causing arthritic symptoms other than metabolic diseases, which are often accompanied by complicated arthritides, for example, pulmonary hypertrophic arthritis, sarcoidosis, ulcerative colitis, regional ileitis, Whipple's disease, liver diseases, hemophilic arthritis, hemoglobinopathy, hemochromatosis, accessory thyroid hyperergasia, hypthyroidism, etc.; diseases causing arthritic symptoms in collagen diseases (except rheumatic arthritis) such as systemic scleroderma (dermatosclerosis), systemic lupus erythematous, etc.; diseases causing arthritic symptoms in Behçet's syndrome; traumatic arthritides and analogous diseases; infectious arthritides such as suppurative arthritis, etc. Experimental tests and results are given hereunder to demonstrate the pharmacological effects of compounds provided by the present invention. (1) Inhibitory Effect on Hypercalcemia: Rats with hypercalcemia induced by administration of parathyroid hormone (hereinafter referred to as "PTH") were used, and the decrement of the serum calcium content due to administration of the compound was measured. Test Method: 30 µg/kg of human 1-34 PTH (manufactured by Peptide Laboratory) which was dissolved in a 0.1% BSA (bovine serum albumin)-containing physiological saline (content of the PTH is 6 µg/ml) was intravenously injected in an amount of 30 µg/kg (5 ml/kg as the solution)into 5-week male Wistar rats which had been fasting for 20 hours. Only 0.1% BSA-containing physiological saline was injected to the normal control group in the same manner. 45 minutes after the PTH injection, the rats were etherized and then subjected to celiotomy, whereby the blood was collected from the abdominal cava with a vacuum blood-collecting tube. The blood collected was immediately centrifuged at 3000 rpm, at 4°C for 10 minutes to isolate the serum. The ionized calcium (Ca⁺⁺) concentration in the serum was immediately measured with a Ca⁺⁺ meter (Sera 250, manufactured by Horiba Manufacturing Co.). The compounds of the present invention were dissolved using sodium hydroxide and hydrochloric acid, in physiological saline (pH 7.4), for subcutaneous administration, in such amounts that the dose amounted to 2 ml/kg; and for oral administration, in distilled water (pH 7.4) so that the dose amounted to 5 ml/kg. They were administered 24 or 72 hours before the PTH injection. Physiological saline and distilled water were administered to the normal control group and the control group, resectively in the same manner. Salmon calcitonin (SCT, manufactured by Armour Co.) was dissolved in physiological saline so that the dose amounted to 2 ml/kg, and then subcutaneously administered 30 minutes before the PTH injection. The results for each group were expressed in terms of mean ± S.E. (standard error) and comparison was made among the groups by testing by one-way analysis of variance. The significance level was taken at 5%. Results: The results obtained by subcutaneous and oral administration are shown in Table 1 and Table 2, respectively. (2) Effect for Inhibition of Bone Resorption: Left forelimbs of rats were immobilized by cutting the left brachial nerve. The inhibitory effect of compounds on the immobilization-induced bone atrophy was demonstrated as mentioned below. Test Method: Five-week Wistar rats were used for the experiment. The bone atrophy was induced as in A.D. Kenny's report (Calcif, Tissue Int., 37, 126-133, 1985). Specifically, the plexus brachialis of the left forelimb of the animal (rat) was cut under thiopental anesthesia so that the left forelimb was immobilized. No treatment was applied to the right forelimb (for control). Rats of a pseudooperation group were treated in the same manner except that the plexus brachialis was not cut. After two weeks, both the left and right humeri were collected. The soft connective tissue was removed from the humeri, the humeri were fully fixed, dehydrated, and defatted with ethanol, and the dry weight of the respective humeri was measured. Afterwards, these were fired at 800°C for 24 hours and the ash weight was measured. The compounds were prepared in the same manner as in Test Method (1) and were subcutaneously administered once a day for 14 days from the day of the operation, as in the Test Method (1). For the control group and the pseudooperation group, physiological saline alone was injected in the same manner. The results for each group were expressed in terms of mean ± S.E. (standard error) and comparison was made among the groups by testing one-way analysis of variance. The significance level was taken at 5%. (3) Antiarthritic Effect: The prophylactic and therapeutic action of compounds of the present invention against arthritis was demonstrated and confirmed by the following test methods. For investigation of the anti-arthritic effect, experimental models of adjuvant arthritis, which resembles rheumatoid arthritis in humans, were used in two test methods. In one method to test the therapeutic effect of the compounds, the compounds to be tested were administered to animals already having the adjuvant arthritis. In the other method to test the prophylactic effect of the compounds, these were administered after the adjuvant administration, and the expression and progress of the resulting arthritis, if any, was observed. Experiment (3)-1: Therapeutic Effect on Adjuvant Arthritis in Rats 0.1 ml of a suspension of dry dead cells of Mycobacterium butyricum suspended in liquid paraffin in a proportion of 6 mg/ml was subcutaneously injected into male Lewis rats (7-week) in the tail. After 17 or 18 days, the thickness of the both hind paws was measured. One group comprising six rats in which arthritis was noticeable was isolated. After the grouping day, the compound to be tested was subcutaneously or orally administered once a day for 14 days to the rats. Next day after the last administration, the thickness of the soles of both hind legs was again measured. Experiment (3)-II: Prophylactic Effect on Adjuvant Arthritis in Rats. 0.05 ml of a suspension of dry dead bacilli of Mycobacterium butyricum suspended in liquid paraffin in a proportion of 6 mg/ml was subcutaneously injected into male Lewis rats (6 to 7-week) in the left hind paw. After the adjuvant injection, the compound to be tested was administered to the rats once a day for 21 days. Next day after the last administration, the thickness of both hind paws of the rats was measured. For Experiments (3)-I and (3)-II, the compounds of the present invention were dissolved, using sodium hydroxide and hydrochloric acid in physiological saline (pH 7.4), for subcutaneous administration, in such amount that the dose amounted to 2 ml/kg, and for oral administration, in distilled water (pH 7.4) so that the dose amounted to 5 ml/kg; and indometacin (as comparative drug) was suspended in 0.5% methyl cellulose solution so that the dose amounted to 5 ml/kg. Each rat was weighed on the first day of administration of the compound to be tested and on the last day of administration of the compound, and the difference in weight (ΔB.W.) was obtained. The results for each group were expressed in terms of mean ± S.E. (standard error) and comparison was made among the groups by testing by one-way analysis of variance. The significance level was taken at 5%. The above-mentioned experimental results apparently demonstrate that the compound of the present invention had an inhibitory effect at least against to 0.01 mg/kg paw swelling by subcutaneous administration in the remedial experiment (Experiment (3)-I) and the ED₅₀ value of the said compound was 0.57 mg/kg. By oral administration, the said compound was also effective in an amount of 100 mg/kg. Further, in the prophylactic experiment (Experiment (3)-II), the compound was also confirmed to be effective by subcutaneous administration. Specifically, this had a significant inhibitory effect in the adjuvant-inoculated paw by subcutaneous administration of 0.1 mg/kg and in the opposite paw with no adjuvant by injection of 0.3 mg/kg. (4) Acute Toxicity Text: The compound of Manufacture Example 1 was subcutaneously injected into rats in an amount of 10 mg/kg/day daily for two weeks, and no rats died. The compounds of the present invention can be blended with any optional pharmaceutically acceptable carrier, vehicle, attenuant, etc. to be formed into powders,granules, tablets, capsules, pills, etc. for oral administration or into injections (for intro-articular injection, etc.), suppositories, inhalations, ointments, etc. for non-peroral administration. The amount of the dose of compounds of the present invention is, although varying in accordance with the administration route, patient's symptom, etc., generally from 10 mg/day/adult to 1 g/day/adult, preferably from 10 to 100 mg/day/adult (for oral administration) and from 0.1 to 100 mg/day/adult for non-oral administration. The following Examples illustrate the present invention in more detail but do not limit it in any way. Manufacture of a raw material compound to be used in Examples is shown in a Reference Example. Some of the raw material compounds were prepared in accordance with the methods described in "Industrial Chemistry" 66 (12), 1831 (1963) (S. Kishimoto) and JP-B-4887/62 (H. Kano et al.). Manufacture of known compounds included in the scope of formula (I) is shown in Manufacture Examples. Reference Example 1 : Raw Material Compound for Example 15 4.7 g of sodium metal was dissolved in 92 ml of ethanol. A mixture of 20 g of 2-heptanone and 26 g of diethyl oxalate was dropwise added thereto at 0°C. After the removing the ice bath, the reaction mixture was stirred at room temperature for 3 hours. After evaporation of the reaction mixture, 200 ml of water was added to the resulting syrup, and then an aqueous 10% hydrochloric acid was added with ice-cooling, the pH being adjusted to 1. After extraction with benzene (300 ml × 3), the organic layer was washed with 100 ml of water, dried with Glauber's salt (Na₂SO₄) and concentrated to give ethyl hexanoylpyruvate as a liquid (38 g). A mixture of 38 g of ethyl hexanoylpyruvate in 190 ml of ethanol in the presence of 14.4 g of hydroxylamine hydrochloride and 16 g of sodium hydrogencarbonate was heated under reflux for 3 hours. After the reaction mixture was cooled to room temperature, the insoluble materials were separated by filtration, and the remaining filtrate was concentrated. The resulting residue was dissolved in chloroform (500 ml), was washed with water, and concentrated to give 5-pentyl-3-carboethoxyisoxazole as a liquid (36 g). A mixture of 36 g of 5-pentyl-3-carboethoxyisoxazole in 105 ml of concentrated aqueous ammonia was vigorously stirred overnight. The reaction mixture was filtered, and the resulting solid was washed with water and dried to obtain 20 g of 5-pentyl-3-carbamidoisoxazole. Subsequently, 19 g of the thus obtained 3-carbamide was added to a solution of 77.5 ml of aqueous 10% sodium hypochlorite containing 8.3 g of sodium hydroxide. The mixture was stirred for 2 hours at room temperature. The reaction mixture was dropped into 60 ml of boiling water for 40 minutes, and then refluxed for a further 40 minutes. The reaction mixture was rapidly cooled to give crystals. The crystals thus formed were filtered and washed with water to obtain 10 g of 3-amino-5-pentylisoxazole. Example 1 : A mixture of 4.9 g of 3-amino-5-methylisoxazole, 8.8 g of ethyl ortho-formate and 13.8 g of diethyl phosphite was heated at 150°C with stirring for 40 minutes. The reaction mixture was cooled to room temperature and the product was purified on a silica-gel column (eluant: 2% methanol-chloroform) to give 4.8 g of tetraethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as an oil. The physico-chemical characteristics of this product are as follows: (i) Mass Spectrum (FAB Mass): 385 (M + 1) (ii) Nuclear Magnetic Resonance (NMR) Spectrum: (in CDCl₃) Example 2 : A mixture of 2.0 g of 3-amino-5-cyclopropyl-isoxazole, 3.0 g of ethyl ortho-formate and 9.1 g of diethyl phosphite was heated at 160°C with stirring for 2.5 hours. The reaction mixture was cooled to room temperature and the product was purified on a silica gel column (eluant: 2% methanol-chloroform) to give 2.5 g of tetraethyl [(5-cyclopropyl-3-isoxazolyl)-amino]methylene-bis(phosphonate) as an oil. The physico-chemical characteristics of this product are as follows: (i) Pale Yellow Oil (at room temperature) (ii) Mass Spectrum (FAB Mass): 411 (M + 1), 365, 273 (iii) NMR Spectrum (in CDCl₃): In the same manner as Example 2, the following compounds were prepared: Example 3 : Tetraethyl [(5-cyclohexyl-3-isoxazolyl)amino]methylene-bis(phosphonate) (i) Yellow Oil (ii) Mass Spectrum (FAB Mass): 453 (M + 1), 407, 315 (iii) NMR Spectrum (in CDCl₃): Starting Compound: 3-Amino-5-cyclohexylisoxazole Example 4 : Tetraethyl [(5-n-propyl-3-isoxazolyl)amino]methylene-bis(phosphonate) (i) Yellow Oil (ii) Mass Spectrum (FAB Mass): 413 (M + 1), 367, 275 (iii) NMR Spectrum (in CDCl₃): Starting Compound: 3-Amino-5-n-propylisoxazole Example 5 : Tetraethyl [(5-phenyl-3-isoxazolyl)amino]methylene-bis(phosphonate) (i) Yellow Oil (ii) Mass Spectrum (FAB Mass): 447 (M + 1), 401, 309 (iii) NMR Spectrum (in CDCl₃): Starting Compound: 3-Amino-5-phenylisoxazole Example 6 : A solution of 2.5 g of tetraethyl [(5-cyclopropyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 25 ml of concentrated hydrochloric acid was heated under reflux for 3.5 hours. The reaction mixture was concentrated. Methanol and acetone were added to the concentrate to give 1.7 g of [(5-cyclopropyl-3-isoxazolyl)-amino]methylene-bis(phosphonic acid) as a solid. (i) m.p.: 176-178°C (ii) Mass Spectrum (FAB Mass): 299 (M + 1), 217 (iii) NMR Spectrum (in D₂O): (iv) Elementary Analysis (as C₇H₁₂N₂O₇P₂ · 0.5H₂O): In the same manner as Example 6, the following compounds were prepared: Example 7: [(5-Cyclohexyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) (i) m.p.: 208-209°C (ii) Mass Spectrum (FAB Mass): 341 (M + 1), 259, 177 (iii) NMR Spectrum (in D₂O): (iv) Elementary Analysis (as C₁₀H₁₈N₂O₇P₂ · 0.3H₂O): Example 8 : [(5-n-Propyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) (i) m.p.: 152-153°C (ii) Mass Spectrum (FAB Mass): 301 (M + 1), 219 (iii) NMR Spectrum (in D₂O): (iv) Elementary Analysis (as C₇H₁₄N₂O₇P₂ · 0.4H₂O): Example 9 : [(5-Phenyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) (i) m.p.: 237-238°C (decomposition) (ii) Mass Spectrum (FAB Mass): 335 (M + 1), 253 (iii) NMR Spectrum (in D₂O with K₂CO₃): (iv) Elementary Analysis (as C₁₀H₁₂N₂O₇P₂): Example 10 : A mixture of 2.2 g of 3-amino-5-ethylisoxazole, 3.4 g of ethyl ortho-formate and 8.1 g of diethyl phosphite was heated at 150-155°C with stirring for 45 minutes. The reaction mixture was concentrated under reduced pressure, and the resulting residue was purified on a silica gel column (eluant: 0.5-2% methanol-chloroform) to give 5.7 g of [(5-ethyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as an oil. The physico-chemical characteristics of this product are as follows: (i) Mass Spectrum (FAB Mass): 399 (M + 1) (iii) NMR Spectrum (in CDCl₃): Example 11 : 6 g of tetraethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) was dissolved in 60 ml of acetic anhydride and heated under reflux overnight. The reaction solution was concentrated under reduced pressure, and the resulting syrup was formed into a chloroform solution. This was washed with water and dried, and then the solvent was removed by distillation. The residue was purified on a silica gel column (eluant: 0.5-2% ethanol-chloroform) to give 5.2 g of tetraethyl (N-acetyl(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as a pale yellow syrup. This had the following physico-chemical properties: (i) Mass Spectrum (FAB Mass): 427 (M + 1) (ii) NMR Spectrum (in CDCl₃): Example 12 : Iodotrimethylsilane (2.68 ml) was added to an ice-cooled solution of 2 g of tetraethyl [N-acetyl(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 20 ml of carbon tetrachloride. Then the temperature was allowed to rise to room temperature, and the mixture was stirred for 1 hour. The reaction mixture was concentrated, methanol was then added, and the mixture was again concentrated. The residue thus obtained was washed with ether, hexane and acetone to give a solid which was recrystallized from acetone-hexane to give 0.5 g of [N-acetyl(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as crystals. The physico-chemical characteristics of this product are as follows: (i) Elementary Analysis (as C₇H₁₂N₂O₈P₂): (ii) Mass Spectrum (FAB Mass): 315 (M + 1) (iii) NMR Spectrum (in D₂O): Example 13 : Iodotrimethylsilane (1.2 ml; 2 molar equivalents) was added dropwise to an ice-cooled solution of 1.6 g of tetraethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 16 ml of carbon tetrachloride. Then the temperature was allowed to rise to room temperature, and the mixture was stirred for 1 hour. The reaction mixture was concentrated, methanol was then added, and the mixture was again concentrated. The residue thus obtained was washed with hexane, and then dissolved in 0.1 N aqueous sodium hydroxide, the pH being adjusted to 7. The solution was applied to an HP-20 resin column for purification (eluant: water) to give 0.2 g of disodium diethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as a solid. The physico-chemical characteristics of this product are as follows: (i) Elementary Analysis (as C₉H₁₆N₂Na₂O₇P₂ · H₂O): (ii) Mass Spectrum (FAB Mass): 327 (M - 1) (iii) NMR Spectrum (in D₂O): Example 14 : Iodotrimethylsilane (1.8 ml; 3 molar equivalents) was added dropwise to an ice-cold solution of 1.6 g of tetraethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 16 ml of carbon tetrachloride. Then the temperature was allowed to rise to room temperature, and the mixture was stirred for 1 hour. The reaction mixture was concentrated, methanol was then added, and the mixture was again concentrated. The residue thus obtained was washed with hexane, and dissolved in water. The solution was applied to an HP-20 resin column for purification (eluant: water) to give 0.27 g of ethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as a solid. The physico-chemical characteristics of this product are as follows: (i) Elementary Analysis (as C₇H₁₄N₂O₇P₂ · 0.5H₂O): (ii) Mass Spectrum (FAB Mass): 301 (M + 1) (iii) NMR Spectrum (in D₂O): Example 15 : A mixture of 2 g of 3-amino-5-n-pentylisoxazole, 2.3 g of ethyl orthoformate and 7.2 g of diethyl phosphite was heated at 150°C with stirring for 60 minutes. The reaction mixture was concentrated under reduced pressure, and the resulting residue was purified on a silica gel column (eluant: 0 to 3% methanol-chloroform) to give 3.2 g of tetraethyl [(5-pentyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as a pale yellow oil. The physico-chemical characteristics of this product are as follows: (i) Mass Spectrum (FAB Mass): 441 (M + 1) (ii) NMR Spectrum (in CDCl₃): In the same manner as Example 15, the following compounds were prepared: Example 16 : Tetraethyl [(5-n-hexyl-3-isoxazolyl)amino]methylene-bis(phosphonate) Physico-chemical properties: (i) Mass Spectrum (EI Mass): 454 (M) (ii) NMR Spectrum (in CDCl₃): Starting Compound: 3-Amino-5-n-hexylisoxazole Example 17 : Tetraethyl [(5-n-heptyl-3-isoxazolyl)amino]methylene-bis(phosphonate) Physico-chemical properties: (i) Mass Spectrum (EI Mass): 468 (M) (ii) NMR Spectrum (in CDCl₃): Starting compound: 3-Amino-5-n-heptylisoxazole Example 18 : Tetraethyl [(5-n-octyl-3-isoxazolyl)amino]methylene-bis(phosphonate) Physico-chemical properties: (i) Mass Spectrum (FAB Mass): 483 (M + 1) (ii) NMR Spectrum (in CDCl₃): Starting compound: 3-Amino-5-n-octylisoxazole Example 19 : Tetraethyl [(5-isoamyl-3-isoxazolyl)amino]methylene-bis(phosphonate) Physico-chemical properties: (i) Mass Spectrum (FAB Mass): 441 (M + 1) (ii) NMR Spectrum Starting compound: 3-Amino-5-isoamylisoxazole Example 20 : Tetraethyl [(5-p-methoxyphenethyl-3-isoxazolyl)amino]methylene-bis(phosphonate) Physico-chemical properties: (i) Mass Spectrum (EI Mass): 504 (M) (ii) NMR Spectrum (in CDCl₃): Starting compound: 3-Amino-5-p-methoxyphenethylisoxazole Example 21 : A solution of 3 g of tetraethyl [(5-n-pentyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 30 ml of concentrated hydrochloric acid was heated under reflux for 3 hours. After the reaction mixture was concentrated, the solid obtained was washed with acetonitrile to give 1.8 g of [(5-n-pentyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as a solid. This had the following physico-chemical properties: (i) Elementary Analysis (as C₉H₁₈N₂O₇P₂): (ii) Mass Spectrum (TAB Mass): 327 (M - 1) (ii) NMR Spectrum (in D₂O with K₂CO₃): In the same manner as Example 21, the following compounds were prepared: Example 22 : [(5-n-hexyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) Physico-chemical properties: (i) m.p.: 238°C (decomposition) (ii) Elementary Analysis (as C₁₀H₂₀N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 343 (M + 1) Example 23 : [(5-n-heptyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) Physico-chemical properties: (i) m.p.: 205°C (decomposition) (ii) Elementary Analysis (as C₁₁H₂₂N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 357 (M + 1) Example 24 : [(5-n-octyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) Physico-chemical properties: (i) m.p.: 225°C (decomposition) (ii) Elementary Analysis (as C₁₂H₂₄N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 371 (M + 1) Example 25 : [(5-isoamyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) Physico-chemical properties: (i) m.p.: 196°C (decomposition) (ii) Elementary Analysis (as C₉H₁₈N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 329 (M + 1) Example 26 : [(5-p-methoxyphenethyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) Physico-chemical properties: (i) m.p.: 265°C (decomposition) (ii) Elementary Analysis (as C₁₃H₁₈N₂O₈P₂): (iii) Mass Spectrum (FAB Mass): 392 (M + 1) Example 27: A mixture of 3 g of 3-amino-5-n-butylisoxazole, 3.7 g of ethyl orthoformate and 12 g of diethyl phosphite was heated at 150°C with stirring for 60 minutes. The reaction mixture was concentrated under reduced pressure, and the resulting residue was purified on a silica gel column (eluant: 0 to 3% ethanol-chloroform) to give 5 g of tetraethyl [(5-n-butyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as a pale yellow oil. The physico-chemical characteristics of this product are as follows: (i) Mass Spectrum (FAB Mass): 247 (M + 1) (ii) NMR Spectrum (in CDCl₃): Example 28 : A solution of 4.8 g of tetraethyl [(5-n-butyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 50 ml of concentrated hydrochloric acid was heated under reflux for 3 hours. After the reaction mixture was concentrated, the solid obtained was washed with acetonitrile to give 2.5 g of [(5-n-butyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as a solid. This had the following physico-chemical properties: (i) m.p.: 200°C (decomposition) (ii) Elementary Analysis (as C₈H₁₆N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 315 (M + 1) Example 29 : A mixture of 3.3 g of 3-amino-5-(4-methyl-3-ene)pentylisoxazole, 4.4 g of ethyl orthoformate and 20 g of diethyl phosphite was heated at 150°C with stirring for 60 minutes. The reaction mixture was concentrated under reduced pressure, and the resulting residue was purified on a silica gel column (eluant: 0 to 3% methanol-chloroform) to give 3.6 g of tetraethyl [[5-(4-methyl-3-ene)pentyl-3-isoxazolyl]amino]methylene-bis(phosphonate) as a pale yellow oil. The physico-chemical characteristics of this product are as follows: (i) Mass Spectrum (EI Mass): 452 (M) (ii) NMR Spectrum (in CDCl₃): In the same manner as Example 29, the following compounds were produced: Example 30 : Tetraethyl [(5-styryl-3-isoxazolyl)amino]methylene-bis(phosphonate) Physico-chemical properties: (i) Mass Spectrum (EI Mass): 472 (M) (ii) NMR Spectrum: Starting compound: 3-Amino-5-styrylisoxazole Example 31: Iodotrimethylsilane (6.2 g) was added dropwise to an ice-cold solution of 3.5 g of tetraethyl [[(5-(4-methyl-3-ene)pentyl-3-isoxazolyl]amino]methylene-bis(phosphonate) in 35 ml of carbon tetrachloride. Then the temperature was allowed to rise to room temperature, and the mixture was stirred for 1 hour. The reaction mixture was concentrated, methanol was then added, and the mixture was again concentrated. The residue thus obtained was washed with acetone and acetonitrile to give 1.8 g of [[5-(4-methyl-3-ene)pentyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as a solid. The physico-chemical characteristics of this product are as follows: (i) m.p.: 213°C (decomposition) (ii) Elementary Analysis (as C₁₀H₁₈N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 341 (M + 1) In the same manner as Example 31, the following compounds were prepared: Example 32 : [(5-styryl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) Physico-chemical properties: (i) m.p.: 287°C (decomposition) (ii) Elementary Analysis (as C₁₂H₁₄N₂O₇P₂): (iii) Mass Spectrum (FAB Mass): 459 (M - 1) Example 33 : A mixture of 2.9 g of 3-amino-4-methylisoxazole, 5.7 g of ethyl orthoformate and 20.4 g of diethyl phosphite was heated at 160°C with stirring for 3 hours. The reaction mixture was concentrated under reduced pressure, and the resulting residue was purified on a silica gel column (eluant: chloroform-ethyl acetate) to give 3.2 g of tetraethyl [(4-methyl-3-isoxazolyl]amino]methylene-bis(phosphonate) as a solid. m.p.: 68-69°C Example 34 : To an ice-cold solution of 9.8 g of 3-amino-5-methylisoxazole in dichloromethane (98 ml) was added dropwise 40 ml of a mixture of formic acid/acetic anhydride (5: 3). Then the mixture was stirred overnight at room temperature. The reaction mixture was concentrated, and the solid obtained was washed with ether to give 10 g of 5-methylisoxazolyl-3-formamide. A mixture of 9.8 ml of trimethyl phosphite and 1.3 ml of phosphorus trichloride was heated at 65°C for 30 minutes. To this reaction mixture was added 1 g of 5-methylisoxazolyl-3-formamide, and the mixture was stirred at that temperature for 1 hour. The reaction mixture was concentrated and subjected to purification on a silica gel column (eluant: chloroform-methanol) to give 0.9 g of tetramethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) as crystals. The physico-chemical characteristics of this product are as follows: (i) Mass Spectrum (FAB Mass): 329 (M + 1) (ii) NMR Spectrum (in CDCl₃): In the same manner as Example 34, the compounds of Examples 2 to 5, 10, 15 to 20, 27, 29, 30 and 33 can also be prepared. Manufacture Example 1 : Iodotrimethylsilane (7.1 ml) was added dropwise to an ice-cold solution of 4.8 g of tetraethyl [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 90 ml of carbon tetrachloride. Then the temperature was allowed to rise to room temperature, and the mixture was stirred for 1 hour. The reaction mixture was concentrated, methanol was then added, and the mixture was again concentrated. The solid thus obtained was washed with hot acetone to give 2.9 g of [(5-methyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as a colorless solid. The physico-chemical characteristics of this product are as follows: (i) Elementary Analysis (as C₅H₁₀N₂O₇P₂): (ii) Mass Spectrum (FAB Mass): 273 (M + 1) (iii) NMR Spectrum (in D₂O): Manufacture Example 2 : A solution of 4.9 g of tetraethyl [(5-ethyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 46 ml of concentrated hydrochloric acid was heated under reflux for 3 hours. After the reaction mixture was concentrated, the solid obtained was washed with acetone to give 2.8 g of [(5-ethyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as a solid. This had the following physico-chemical properties: (i) Elementary Analysis (as C₆H₁₂N₂O₇P₂): (ii) Mass Spectrum (FAB Mass): 287 (M + 1) (iii) NMR Spectrum (in D₂O): Manufacture Example 3 : A solution of 3.2 g of tetraethyl [(4-methyl-3-isoxazolyl)amino]methylene-bis(phosphonate) in 32 ml of concentrated hydrochloric acid was heated under reflux for 4 hours. After the reaction mixture was concentrated, the solid obtained was washed with a mixture of methanol-acetonitrile-acetone to give 1.8 g of [(4-methyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) as a solid. (i) m.p.: 272-274°C (decomposition) (ii) Elementary Analysis (as C₅H₁₀N₂O₇P₂): Prescription Example : Examples for prescription of compounds of the present invention as drugs are mentioned below. (1) Tablet: 5 g of the compound of Manufacture Example 1, 119 g of lactose and 67 g of corn starch were uniformly blended, 40 ml of an aqueous 10% (w/w) hydroxypropyl cellulose solution was added thereto, and the resulting mixture was wet-granulated. The granules thus obtained were blended with 4 g of calcium carboxymethyl cellulose and 1 g of magnesium stearate, and the resulting mixture is formed into tablets having a weight of 200 mg/tablet. (2) Capsule: The above-mentioned ingredients were blended in amounts 1000 times those mentioned above, and were encapsulated in gelatin capsules, each containing 200 mg of the mixture.

Claims: Claims for the following Contracting States: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, and SE 1. A bisphosphonic acid derivative of formula (I′) or a salt thereof in which R¹ is a C₁-C₁₀ alkyl group, a C₃ to C₁₀ cycloalkyl group, a phenyl group, a C₂ to C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅) alkyl group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups, provided that when R¹ is a methyl, ethyl, isopropyl, or tert-butyl group at least one of R², R³, R⁴, R⁵ and R⁶ is not a hydrogen atom. 2. A compound according to claim 1 wherein R¹ is a methyl, pentyl or cyclopropyl group. 3. At least one compound according to claim 1 selected from [(5-n-pentyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) and salts thereof; tetraethyl[(5-n-pentyl-3-isoxazolyl)amino]methylene-bis(phosphonate); [(5-cyclopropyl-3-isoxazolyl)amino]methylene-bis(phosphonic acid) and salts thereof; and tetraethyl[(5-cyclopropyl-3-isoxazolyl)amino]methylene-bis(phosphonate). 4. A pharmaceutical composition containing at least one compound selected from those of formula (I) and pharmaceutically acceptable salts thereof in which R¹ is a C₁-C₁₀ alkyl group, a C₃ to C₁₀ cycloalkyl group, a phenyl group, a C₂ to C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅) alkyl group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups. 5. A composition according to claim 4 wherein R¹ is a C₁-C₅ alkyl group or a C₃-C₁₀ cycloalkyl group. 6. A composition according to claim 4 containing at least one compound selected from [(5-methyl-3-isoxazolyl)amino]methylene bis(phosphonic acid), pharmaceutically acceptable salts thereof, and the compounds of claim 2 or 3. 7. The use of at least one compound selected from those of formula (I) and pharmaceutically acceptable salts thereof (in which R¹ is a C₁-C₁₀ alkyl group, a C₃ to C₁₀ cycloalkyl group, a phenyl group, a C₂ to C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅) alkyl group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups) for the preparation of a medicament having anti-arthritic or bone resorption-inhibiting activity. Claims for the following Contracting States: ES and GR 1. A process for producing a bisphosphonic acid derivative of formula (I′) or a salt thereof, wherein R¹ is a C₁-C₁₀ alkyl group, a C₃ to C₁₀ cycloalkyl group, a phenyl group, a C₂ to C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅) alkyl group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is a hydrogen atom or a C₂-C₆ alkyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups, provided that when R¹ is a methyl, ethyl, isopropyl, or tert-butyl group at least one of R², R³, R⁴, R⁵ and R⁶ is not a hydrogen atom; which comprises reacting 3-aminoisoxazole compound (II) lower alkyl ortho-formate (III) (wherein R⁷, R⁸ and R⁹ are the same or different C₁-C₅ alkyl group(s)) and dialkyl phosphite (IV) (wherein R 3′ , R 4′ and R 6′ are the same or different C₁-C₅ alkyl group(s)), and optionally acylating and/or hydrolyzing the reaction product and/or converting to or from salt form. 2. A process according to claim 1 wherein compound (II) is 3-amino-5-n-pentyl isxazole; 3-amino-5-cyclopropyl isoxazole; or 3-amino-5-methyl isoxazole. 3. A process for producing a bisphosphonic acid derivative of formula (I′) or a salt thereof wherein R¹ is a C₁-C₁₀ alkyl group, a C₃ to C₁₀ cycloalkyl group, a phenyl group, a C₂ to C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅) alkyl group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group; R² is a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups, provided that when R¹ is a methyl, ethyl, isopropyl or tert-butyl group at least one of R², R³, R⁴, R⁵ and R⁶ is not a hydrogen atom; which comprises reacting substituted isoxazole-3-formamide (VIII) with the reaction product of phosphorus trihalogenide PX₃ (wherein X is halogen) and trialkyl phosphite (VII) (wherein R 3′ , R 4′ , R 5′ and R 6′ are the same or different C₁-C₅ alkyl group(s), and optionally acylating and/or hydrolyzing the reaction product and/or converting to or from salt form. 4. A process according to claim 1 or 3 wherein R¹ represents a methyl, pentyl, or cyclopropyl group. 5. A process according to claim 3 wherein compound (VIII) is 5-n-pentyl isoxazolyl-3-formamide; 5-cyclopropyl isoxazolyl-3-formamide; or 5-methyl isoxazolyl-3-formamide. 6. The use of at least one compound selected from those of formula (I) and pharmaceutically acceptable salts thereof (in which R¹ is a C₁-C₁₀ alkyl group, a C₃ to C₁₀ cycloalkyl group, a phenyl group, a C₂ to C₁₀ alkenyl group which may be substituted by a phenyl group, or a phenyl substituted (C₁-C₅) alkyl group in which the phenyl group may be substituted by a C₁-C₅ alkoxy group;: R² is a hydrogen atom or a C₂-C₆ alkanoyl group; and R³, R⁴, R⁵ and R⁶ are the same or different and selected from a hydrogen atom and C₁-C₅ alkyl groups) for the preparation of a medicament having anti-arthritic or bone resorption-inhibiting activity.

Inventors: ABE, TETSUSHI, ISOMURA, YASUO, KAWAMUKI, KOUSEI, KUDOU, MASAFUMI, NIIGATA, KUNIHIRO, SAKAMOTO, SHUICHI, TAKEUCHI, MAKOTO
Applicants: YAMANOUCHI PHARMACEUTICAL CO. LTD.
Application year: 1988
Main classification: C07F9
IPC codes: C07F9