STEROID CREAM FORMULATION

Abstract: A steroid cream formulation which has enhanced physical and chemical stability is formed of (11β,17α)-17-(ethylthio)-9α-fluoro-11β-hydroxy-­ 17-(methylthio)androsta-1,4-dien-3-one (tipredane), and a vehicle containing as major ingredients glyceryl monostearate having an acid value of greater than 4, propylene glycol and water together with a sodium citrate or potassium citrate buffer, a non-acidic long chain fatty acid wax to impart proper consistency, and sodium metabisulfite as an antioxidant, together with emulsifiers, skin conditioners, emollients, lubricants and other conventional cream formulation ingredients.

Description: The present invention relates to a steroid cream formulation which has enhanced physical and chemical stability and contains (11β,17α)-17-(ethylthio)-9α-fluoro-11β-hydroxy-17-(methylthio)-androsta-1,4-dien-3-one (tipredane) as the active ingredient. U. S. Patent No. 4,361,559 to Varma discloses antiinflammatory 17,17-bis(substituted thio)androstenes of the formula wherein R₁ and R₂ are the same or different and each is alkyl, cycloalkyl or aryl; R₃ is hydrogen, hydroxy, alkoxy, aryloxy, alkylthio, arylthio, or halogen; R₄ is hydrogen, methyl, hydroxy, or halogen; R₅ is hydrogen or halogen; and R₆ is carbonyl or β-hydroxymethylene. A broken line in the 1,2-, 6,7-and 15,16-positions of a structural formula indicates the optional presence of ethylenic unsaturation. Included among the compounds covered in the Varma patent is tipredane which has been found to be a highly effective topical antiinflammatory agent. Tipredane is practically insoluble in water (less than 0.0002 mg/ml at 25°C); 1:1 hydroalcoholic mixtures of tipredane are unstable under acidic conditions. Further, tipredane itself is susceptible to oxidation. It is known to use monoglycerides such as glyceryl monostearate as a thickener in steroid cream formulations. For example, U. S. Patent No. 3,892,857 to DiFazio et al discloses a cream formulation containing propylene glycol, water and a steroid, namely, 21-chloro-9α-fluoro-Δ⁴-pregnene-11β,16α,17α-triol-3,20-dione 16,17-acetonide (halcinonide) and as an oleaginous thickener monoglycerides such as glyceryl monostearate, glyceryl monooleate, glyceryl monopalmitate and glyceryl ricinoleate. Where it has been attempted to employ generally commercially available glyceryl monostearate in an oil-in-water tipredane cream formulation, it has been found that syneresis of the aqueous phase occurs. Another problem associated with formulating of oil-in-water tipredane creams is obtaining proper consistency without sacrificing chemical and physical stability of the tipredane. In accordance with the present invention, a topical oil-in-water steroid cream formulation is provided which contains the steroid tipredane as its active ingredient and has excellent physical and chemical stability and does not undergo syneresis although it contains glyceryl monostearate. The oil-in-water cream formulation according to the present invention contains in addition to tipredane, a carrier vehicle which is formed of one or more solubilizers for the tipredane, water, one or more emulsifiers including glyceryl monostearate (which has an acid value of greater than 4), one or more buffers to impart a neutral or slightly alkaline pH, and preferably within the range of 5 to 9, optionally one or more emollients, optionally one or more metal chelating agents, optionally one or more skin conditioners, optionally one or more preservatives, and optionally one or more silicone lubricants or defoaming agents. In addition, in order to obtain acceptable consistency and chemical stability, the cream formulation of the invention will include a non-acidic long chain fatty acid ester wax to impart desired consistency to the cream so that the cream remains in solid form at temperatures as high as 40°C, such as Syncrowax ERLC (trademark of Croda) which is ethylene glycol ester of a long chain (C₁₈-C₃₆) fatty acid wax. It has been found that such non-acidic wax will impart the desired consistency to the cream but will not interfere with the stability of the tipredane. The tipredane steroid will be employed in the form of a micronized powder having an average particle size of less than about 75 µm and will be present in an amount within the range of from 0.005 to 0.5% by weight and preferably from 0.05 to 0.3% by weight based on the total weight of the tipredane cream formulation to supply from 0.5 to 3 mg/g activity. As indicated, the carrier vehicle will contain one or more solubilizers for the tipredane such as propylene glycol (which also serves as a preservative, lubricant, humectant and emollient), polysorbate® 60, benzyl alcohol or mixtures thereof. The propylene glycol and/or other solubilizers will be present in an amount within the range of from 5 to 30% by weight and preferably from 10 to 20% by weight of the cream formulation. Water which serves as a vehicle for the cream formulation and a solubilizer for salts present in the formulation, will be present in an amount within the range of from 30 to 70% and preferably from 40 to 65% by weight of the total cream formulation. An important feature of the cream formulation of the invention is its excellent chemical stability and physical stability. Syneresis will not occur if glyceryl monostearate having acid value of greater than 4 is used. However, if low acid value glyceryl monostearate is used, the cream will show syneresis initially, but if the bulk cream is stored for a period of 1 to 3 weeks, it firms up in consistency and syneresis no longer occurs. Further, low acid value glyceryl monostearate may be used, for example, acid value of 0.5 to 4, in which case stearic acid or other acid is employed in conjunction therewith to bring its acid value to greater than 4. A buffer is included to impart a pH to the cream of within the range of from 5 to 9 and preferably from 6.5 to 8.5 as so to ensure chemical stability of the tipredane. Examples of buffers suitable for use herein include sodium citrate, potassium citrate in the form of the monohydrate, or an alkali metal hydroxide, such as sodium hydroxide or potassium hydroxide or magnesium hydroxide or aluminum hydroxide. It is preferred to use a mixture of buffers such as sodium citrate and aluminum hydroxide (2:1 to 0.5:1). The cream formulation will include a thickener which is a non-acidic long chain fatty wax (as described above) in an amount within the range of from 0.5 to 5 weight % and preferably from 1 to 3 weight % based on the weight of total tipredane cream formulation. Examples of non-acid long chain fatty waxes suitable for use herein include, but are not limited to Syncrowax® ERLC (described above), white wax, carnauba wax, paraffin wax or ceresin wax. The emulsifier will be present in an amount within the range of from 2 to 8 weight % and preferably from 3 to 7 weight % based on the weight of the total tipredane cream formulation. Examples of emulsifiers suitable for use herein include but are not limited to polyethylene oxide 60 sorbitan monostearate (polysorbate® 60), polysorbate® 20, polysorbate® 65, polysorbate® 80, or Promulgen® D (cetearyl alcohol and ceteareth-20® (3:1)). The cream formulation of the invention will also include an emulsion stabilizer such as glyceryl monostearate, cetyl alcohol or stearyl alcohol in an amount within the range of from 5 to 25% by weight, and preferably from 7 to 22% by weight. It is preferred to employ a mixture of such emulsion stabilizers such as, glyceryl monostearate in an amount of within the range of from 5 to 15% by weight and preferably from about 7 to about 13% by weight, and cetyl alcohol in an amount within the range of from 2 to 8% by weight and preferably from 3 to 7% by weight. All of the above % are based on the total weight of the tipredane cream formulation. The emollients may be present in an amount within the range of from 1 to 6% and preferably from 2 to 4% by weight based on the total tipredane cream formulation. Examples of emollients suitable for use herein include but are not limited to mineral oil, lanolin alcohol, a mixture of mineral oil and lanolin alcohol (9:1) as sold under the trademark Amerchol L-101 (Amerchol Corp, a unit of CPC International). Examples of other emollients suitable for use herein include, but are not limited to cetyl alcohol, isopropyl isostearate, isopropyl myristate, isopropyl palmitate or octyl dodecyl stearate. The cream formulation may optionally include one or more skin conditioners of humectants in an amount of within the range of from 0.5 to 4% and preferably from 1 to 3% by weight based on the total tipredane cream formulation, such as an alkoxylated methyl glucose derivative, for example, polypropylene glycol-20 methyl glucose ether (Glucam P-20, trademark of Amerchol Corp, CPC Int.), polypropylene glycol-10 methyl glucose ether (Glucam P-10, trademark of Amerchol Corp, CPC Int.) and polyethylene glycol ether of methyl glycose (Glucam E-10 or E-20). Other skin conditioners that may be employed include allantoin, d- or dl-panthenol, and sodium 2-pyrrolidone carboxylic acid. In addition, the cream formulation will include a lubricant or defoamer in an amount within the range of from 0.1 to 3% by weight and preferably from 0.5 to 2% by weight based on the total tipredane cream formulation. Examples of such lubricants suitable for use herein include, but are not limited to silicones such as dimethicone (Silicone DC 200 Fluid® ((3,50.cm²/s) 350 CSt)), polyphenylmethylsiloxane or polydimethylsiloxane. The cream formulation of the invention will include an antioxidant, such as sodium metabisulfite, butylated hydroxytoluene, butylated hydroxyanisole or sodium ascorbate in an amount within the range of from 0.01 to 1% by weight and preferably from 0.02 to 0.5% by weight of the tipredane cream formulation. As the optional chelating agent disodium or dipotassium ethylenediamine tetraacetate dihydrate is preferred. Other examples of metal chelating agents which may be employed include citric acid, fumaric acid or malic acid. The metal chelating agent will be employed in an amount within the range of from 0.001 to 0.5% by weight and preferably from 0.002 to 0.1% by weight of the tipredane cream formulation. The following represents preferred oil-in-water cream formulations in accordance with the present invention. Ingredient Range Percent w/w Tipredane (in form of micronized powder) (<75µm) 0.05 to 0.3 Glyceryl monostearate (emulsion stabilizer) 7 to 13 Propylene glycol (solubilizer-preservative) 10 to 20 Sodium citrate (buffer) 0.3 to 0.7 Sodium metabisulfite (antioxidant) 0.02 to 0.5 Disodium EDTA dihydrate (chelating agent) 0.002 to 0.1 Aluminum hydroxide conc. wet gel (buffer) 0.10 to 0.60 Syncrowax® ERLC (non-acidic long chain fatty acid wax-thickener) 1 to 3 Cetyl alcohol (emulsion stabilizer) 3 to 7 Mineral oil-lanolin alcohol combination (9:1) (emollient) 2 to 4 Methyl gluceth®-20 (skin conditioner) 1 to 3 Polysorbate® 60 (emulsifer) 3 to 7 Silicone fluid (lubricant or defoamer) 0.5 to 2 Water 40 to 65 The tipredane cream formulation of the invention may be prepared as described in the working Example by simply forming an aqueous phase (containing methyl gluceth-20®, polysorbate-10®, propylene glycol, sodium citrate, EDTA and sodium metabisulfite, aluminum hydroxide and silicone fluid) and an oil phase (containing glyceryl monostearate cetyl alcohol, mineral oil-lanolin alcohol, Syncrowax®) and mixing the two phases until a homogeneous oil-in-water emollient cream is obtained. The following Example represents a preferred embodiment of the present invention. Unless otherwise indicated, all temperatures are expressed in degrees Centigrade. Example A 0.1% w/w tipredane oil-in-water formulation having the following composition was prepared as described below. Tipredane Cream 0.1 W/W Ingredient Amount (mg) Tipredane micronized powder (20 µm) (to supply 1.0 mg/g activity) 1 Glyceryl monostearate (emulsion stabilizer) 110 Cetyl alcohol NF (emulsion stabilizer) 40 Syncrowax® ERLC (Croda) (wax for proper consistency) 15 Amerchol® L-101 (mineral oil and lanolin alcohol [9:1])(emollient) 30 Glucam® E-20 (methyl gluceth®-20)(skin conditioner) 20 Tween 60 (polysorbate® 60)(emulsifier) 40 Propylene glycol USP (solubilizer-preservative) 150 Silicone® DC200 fluid (3,50 cm²/s) (350CS) (lubricant-defoamer) 10 Sodium citrate USP (buffer) 5 Disodium edetate dihydrate USP (chelating agent) 0.05 Sodium metabisulfite (antioxidant) 0.2 Aluminum hydroxide conc. wet gel (buffer) 4 Purified water USP (or equivalent) ca. 575 sufficient quantity to make 1 g A mixture of glyceryl monostearate and cetyl alcohol was heated to 60 to 65° to melt same. Syncrowax® ERLC was added with agitation and the mixture was heated to 80 to 85°C. The mineral oil-lanolin alcohol (Amerchol® L-101) was added with agitation and the so-formed oil phase was maintained at 80 to 85°C until it was ready to add same to the aqueous phase. The water phase was prepared by mixing methyl gluceth®-20, polysorbate® 60, propylene glycol (80% of total propylene glycol), sodium citrate, disodium edetate dihydrate and sodium metabisulfite with agitated purified water and heating to 80 to 85°C. Aluminum hydroxide concentrated wet gel was added to a portion of purified water; the resulting slurry was passed through a colloid mill to form a smooth dispersion which was added to the above aqueous solution at 80 to 85°C. The oil phase was heated at 90-95°C and added to the aqueous phase at 90-95°C with homogenization to form an oil in water emulsion. The batch was cooled to 70-75°C with continuous homogenization. Silicone fluid was added and the batch was cooled. The weight of the batch was adjusted with hot purified water (54-56°C) while maintaining continuous homogenization to prevent phase separation. The batch was cooled to about 50°C to 52°C or until onset of congealing. Homogenization was stopped and the batch was cooled to 25-30°C using planetary type mixing. A slow rate of cooling (not greater than 1°C per 15 minutes) was used during congealing phase from 49°C to 44°C. The batch was allowed to cool below 44°C by standing overnight without mixing until cooled to 25-30°C. Tipredane was dispersed in the remainder of the propylene glycol using a colloid mill homogenizer and added to the batch at 25-30°C. The final batch weight was adjusted with purified water. The mix was mixed an additional 30 minutes until homogeneous. The above tipredane cream formulation was found to have excellent chemical and physical stability and did not undergo syneresis even after prolonged periods of storage at temperatures of 5°C, 25°C and 40°C.

Claims: Claims for the following Contracting States : AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE A tipredane cream formulation having enhanced chemical and physical stability, having the formula %w/w tipredane micronized powder 0.10% propylene glycol 15% sodium citrate 0.5% disodium EDTA dihydrate 0.005% sodium metabisulfite 0.02% glyceryl monostearate having an acid value greater than 4 11% cetyl alcohol 4% ethylene glycol ester of a long chain (C₁₈-C₃₆) fatty acid wax 1.5% mineral oil-lanolin alcohol (9:1) 3% methyl gluceth®-20 2% polysorbate® 60 4% silicone fluid 1% aluminum hydroxide conc. wet gel 0.4% purified water 57.5% . The use of the following components at the following percentages by weight: %w/w tipredane micronized powder 0.10% propylene glycol 15% sodium citrate 0.5% disodium EDTA dihydrate 0.005% sodium metabisulfite 0.02% glyceryl monostearate having an acid value greater then 4 11% cetyl alcohol 4% ethylene glycol ester of long chain (C₁₈-C₃₆) fatty acid wax 1.5% mineral oil-lanolin alcohol (9:1) 3% methyl gluceth®-20 2% polysorbate® 60 4% silicone fluid 1% aluminum hydroxide conc. wet gel 0.4% purified water 57.5% for preparing a tipredane cream formulation for topical treatment of dermatitis. Claim for the following Contracting States : ES, GR The use of the following components at the following percentages by weight: %w/w tipredane micronized powder 0.10% propylene glycol 15% sodium citrate 0.5% disodium EDTA dihydrate 0.005% sodium metabisulfite 0.02% glyceryl monostearate having an acid value greater then 4 11% cetyl alcohol 4% ethylene glycol ester of a long chain (C₁₈-C₃₆) fatty acid wax 1.5% mineral oil-lanolin alcohol (9:1) 3% methyl gluceth-20® 2% polysorbate® 60 4% silicone fluid 1% aluminum hydroxide conc. wet gel 0.4% purified water 57.5% for preparing a tipredane cream formulation for topical treatment of dermatitis.

Inventors: O'LAUGHLIN, RICHARD L., PANAGGIO, ANDREA, VARIA, SAILESH AMILAL
Applicants: E.R. SQUIBB & SONS, INC.
Application year: 1988
Main classification: A61K31
IPC codes: A61K47, A61P29, A61K9, A61K31