Methoxymethyl compounds

Abstract: A substantially (≧95%) optically pure (5R,6S,1′R) penem of formula and the pharmaceutically acceptable salts and the ester prodrugs thereof, are endowed with antibacterial activity.

Description: The present invention relates to penem ester prodrugs which, in vivo , release the (5R,6S,1'R)-configured 6-(1-hydroxyethyl)-2-methoxymethyl-penem-3-carboxylic acid represented by the formula: The compound of formula (I) is an antibacterial agent. The penem ester prodrugs are therefore useful in the treatment of infections in humans and mammalian species. EP-A-0 199 446 discloses the (1'R,5R,6S) and (1'S,5S,6R) racemic mixture of the allyl ester of the acid of formula (I) but does not refer to optically pure isomers. The present invention now provides a substantially (≧95%) optically pure (5R,6S,1'R) penem ester prodrug of the formula: wherein R is a) acyloxymethyl or 1-(acyloxy)ethyl; b) benzoyloxymethyl or 1-(benzoyloxy)ethyl, either unsubstituted or substituted on the ring by a free, methylated or acetylated hydroxy or amino group; c) alkoxycarbonloxymethyl or 1-(alkoxycarbonyloxy)ethyl; d) 3-phthalidyl; e) 2-oxo-1,3-dioxolan-4-yl, optionally substituted by a C₁-C₄ alkyl group in the 5 position; f) (2-oxo-1,3-dixolen-4-yl)methyl, optionally substituted by a phenyl or C₁-C₄ alkyl group at the 5 position; g) a group CH₂CO₂R', wherein R' is C₁-C₄ straight or branched alkyl, or benzyl; or h) 2-oxotetrahydrofuran-5-yl, optionally substituted by a C₁-C₄ alkyl group at the 4 position. The (5R,6S,1'R)-configured product constitutes at least 95% of any mixture of possible stereoisomers thereof. The term "ester prodrugs" means that the esters of the formula (II) can be cleaved under physiological conditions, releasing the parent penem carboxylic acid of formula (I) in vivo . In particular, the term is used with respect to esters which can be absorbed from the gastro-intestinal tract after oral administration, and then are hydrolyzed in the bloodstream by aspecific serum esterases. In the definition of R under a) in formula (II) above the term "acyl" is intended to include straight or branched C₂-C₁₀ alkanoyl or C₄-C₈ cycloalkanoyl groups. Particularly preferred ester prodrugs of formula (II) are those herein tabulated: The compounds of the present invention can be manufactured by the following process comprising: i) cyclising a compound of formula (III) wherein P' is either hydrogen or P'' where P'' is a hydroxy protecting group, and R² is either R as defined for formula (II) above or is a carboxy protecting group; ii) cyclising a compound of formula (IV) wherein P' and R² are as defined above, and Y represents either oxygen or sulfur; iii) if necessary removing the optional protecting groups P'' and R² from the resultant compound, and converting the resulting compound of formula (I) as defined above or salt thereof into a pharmaceutically acceptable ester prodrug of formula (II) as defined above by treatment with a compound of formula (V): R-X (V) wherein R is as defined above and X is either a chloro, bromo or iodo atom or a mesyloxy, trifluoromethanesulfonyloxy or tosyloxy group. Preferred protecting groups P'' for the hydroxyl function are trimethylsilyl, tert-butyldimethylsilyl, tetrahydropyranyl, allyloxycarbonyl or p-nitrobenzyloxycarbonyl. When R², being different from R, is a carboxy protecting group, it is preferably allyl, p-nitrobenzyl or p-methoxybenzyl. The conditions for the removal of said protecting groups are known per se . The cyclization of a compound of formula (III) is carried out by plain heating in an inert organic solvent,preferably benzene , toluene or dioxane, at reflux or near-to-reflux temperatures. The cyclization of a compound of formula (IV) is carried out by treatment with trimethylphosphite or triethylphosphite in an inert organic solvent, such as chloroform, benzene, toluene, xylene or dioxane. The conditions for said cyclization, which depends on whether Y is oxygen or sulfur, are known- per-se and detailed in C. Battistini et al., Tetrahedron Lett. 25, 2595 (1984) and A. Yoshida et al., Chem. Pharm. Bull. 31 , 768 (1983), and references therein. The reaction of a compound of formula (I), or a salt thereof,with a compound of formula (V) is carried out in an inert organic solvent, preferably dimethylformamide, tetrahydrofuran, N-methylpyrrolidone, or dimethylsulfoxide, at temperatures ranging from -10°C to +40°, preferably from 0°C to +25°C, optionally in the presence of a base, such as sodium hydrogen carbonate, potassium carbonate, triethylamine or pyridine. The intermediates of formulae (III), (IV) may be obtained by the following azetidinone precursors: wherein P' and R² are as defined above, and L is a leaving group, preferably acetoxy, benzyloxy, phenylsulfonyl or chloro, by known-per-se methodologies. They are summarized in the following scheme: The compounds of formula (V),(VI),(VII),(VIII) are known or can be obtained from known compounds by known-per-se methodologies. The parent compound of formula (I) has high antibacterial activity against gram-positive and gram-negative bacteria, combined with good pharmacokinetic properties in vivo in humans and mammals. Owing to these properties, and to its quite negligible toxicity, an excellent therapeutic index can be attained in the treatment of infectious diseases caused by said micro-organisms. We have found that the optical purity of 6-(1'-hydroxyethyl)-2-methoxymethylpenem-3-carboxylic acid derivatives, in terms of the proportion of the (5R,6S, 1'R)-configured isomer present in an isomeric mixture, plays an essential role in the potency, width of spectrum and chemoenzymatic stability of the product. Thus the sodium salt of the parent compound of formula (I), when compared with the complex mixture of racemic streoisomers disclosed in US-A-4,272,437, prepared as therein described (Example 36), shows an in vitro activity increased by a factor from 8 to 30, depending on the strain tested (Table 2) . Moreover, compared with the prior-described mixture, the pure (5R,6S,1'R)-configured compound of formula (I) showed superior stability towards bacterial β-lactamases and renal dehydropeptidases, and a considerably improved chemical stability over the whole pH-range. These findings were confirmed by in vivo experiments (Table 3); indeed, while the sodium salt of the compound of formula (I) showed excellent efficacy in the control and treatment of experimental infections caused by gram-positive and gram-negative bacteria in the mouse, under the same experimental conditions the prior-art mixture showed no therapeutically useful levels of antimicrobial activity. When the two products were analyzed and compared, it was found that the prior art mixture contained the (5R,6S,1'R)-configured epimer in such a minute amount to be hardly measurable by NMR integration. This finding is consistent with the results published for the epimeric mixture of a similar penem obtained by the same authors through the same route: Y.Ueda, A. Martel, J.-P. Daris, B. BeIleau and M. Menard, Can. J. Chem. 60 ,904,1982. Thus, the major component of the prior art product was found to be the 5,6-cis-1'R racemate, i.e. an equimolecular amount of the 5R,6R,1'R and 5S,6S,1'S enantiomers. The compounds of formula (II), being the ester prodrugs of the active compound of formula (I), offer the advantage of a very favourable bioavailability following oral administration. Their superior absorption from the gastro-intestinal tract, coupled with the good pharmacokinetic parameters of the compound of formula (I) that is relased in vivo , results in higher and more prolonged plasma levels when compared with other penem ester prodrugs, for example with the most advanced compound of this type, FCE 22891 (G. Franceschi et al., J. Antibiotics 36 , 938, 1983). This is apparent from Table 4, where the compounds n°1 and n°20 of the present invention are compared with the corresponding ester prodrugs of FCE 22101, namely FCE 22891 and FCE 23761. The present invention includes pharmaceutical preparations for human or veterinary use containing the ester prodrugs of formula (II). For oral administration, there are used tables or gelatine capsules that contain a prodrug ester, preferably selected from those encompassed by the formula (II), still preferably selected from those listed in Table 1, together with diluents, for example lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and/or glycine, and lubricants, for example silica, talc, stearic acid or salts thereof, such as magnesium or calcium stearate, and/or polyethylene glycol; tablets also contain binders, for example magnesium aluminium silicate, starches, such as corn, wheat, rice or arrowroot starch, gelatine, tragacanth, methylcellulose, sodium carboxymethylcellulose and/or polyvinylpyrrolidone, and, if desired, disintegrators, for example starches, agar, alginic acid or a salt thereof, such as sodium alginate, and/or effervescent mixtures or adsorbents, colourings, flavourings or sweeteners. For parenteral administration there are used infusion solutions, preferably isotonic aqueous solutions or suspensions being it possible to prepare these before use, for example from lyophilised preparations, that contain the compound of formula (I),or a pharmaceutically acceptable salt thereof, preferably selected from the group consisting of sodium, potassium or arginine salt, which may be present on its own or together with a carrier, for example mannitol. Such preparations may be sterilised and/or contain adjuncts, for example preservatives, stabilisers, wetting agents and/or emulsifiers, solubilisers, salts for regulating the osmotic pressure and/or buffers. The present pharmaceutical preparations, which, if desired, may contain other pharmacologically valuable substances, are manufactured in a manner known per se, for example by means of conventional mixing, dissolving or lyophilising processes, and contain from approximately 0.1% to 100%, especially from approximately 1% to approximately 50% or, in the case of lyphilisates, up to 100%, active ingredient. Depending upon the type of infection and the condition of the infected organism, the daily dose used for the treatment of a warm-blooded animal (human or animal) weighing approximately 70 kg is from 125 mg to approximately 5 g. Reference Example Allyl (5R,6S)-6-[1(R) tert.Butyldimethylsilyloxy-ethyl]-2-MethoxymethylPenem-3-Carboxylate (3S,4R)-1-[1-(Allyloxycarbonyl)-1-(Triphenylphosphoranyliden)methyl]-3-[1(R) tert.butyldimethylsilyloxyethyl]-4-(argentothio)-azetidin-2-one (727 mg) was dissolved in dry CH₃CN (20 ml) and treated with methoxyacetyl chloride (110 ul). After stirring for 10 minutes at r.t, the reaction mixture was diluted with ethyl acetate, filtered through celite, washed with 5% aqueous sodium hydrogen carbonate then twice with brine. "r.t." means room temperature. After evaporating the solvent in vacuo, the residue was taken up in toluene (150 ml) and heated at reflux for 90 minutes. The solution was concentrated under reduced pressure then chromatographed (23)-400 Mesh Silica Gel; cyclohexane-ethyl acetate 80/20 as eluant) affording the title product as a yellowish oil (360 mg; 87%) IR (CHCl₃)ν 1785, 1700 cm⁻¹ NMR (90 MHz, CDCl₃)δ : 0.09 (6H, s) 0.89 (9H, s) 1.26 (3H, d, J=6.5 Hz) 3.39 (3H, s) 3.67 (1H, dd, J= < 2 and 7 Hz) 4.23 (1H, m) 4.5-4.8 (4H, m) 5.0-5.5 (2H, m) 5.56 (1H, d, J < 2Hz) 5.55-6.05 (1H, m) Reference Example Allyl (5R,6S)-6-[1(R)Hydroxyethyl]-2-MethoxymethylPenem-3-Carboxylate A solution of allyl (5R,6S)-6-[1(R) tert.butyldimethylsilyloxyethyl]-2-methoxymethylpenem-3-carboxylate (360 mg) in dry tetrahydrofuran (6 ml) was sequentially treated with acetic acid (0.6 ml) and tetrabutylammonium fluoride trihydrate (930 mg) under stirring. The solution was let stand overnight at r.t., then concentrated to a small volume and chromatographed on silica gel (cyclohexane/ethyl acetate 1/1 as eluant) to give the title product as white powder (250 mg) UV (CHCl₃) λ max 326 nm. Reference Example Allyl (5R,6S)-6-[1(R)Hydroxyethyl]-2-MethoxymethylPenem-3-Carboxylate A solution of (3S,4R)-1-[1-(allyloxycarbonyl)-1-(triphenylphosphoranyliden) methyl]-3-[1(R)hydroxyethyl]-4-(argentothio)-azetidin-2-one (6.1 g) in dry acetonitrile (250 ml) at -10°C was treated with methoxyacetylchloride (1.2 ml), then stirring was continued for 15 minutes at 0°C. Ethyl acetate was added and the resulting mixture was filtered over celite. The organic phase was washed with aqueous sodium hydrogen carbonate, dried over sodium sulphate and concentrated. Flash chromatography of the residue (Silica gel 230-400 Mesh; hexaneethyl acetate mixtures as eluants) gave (3S,4R)-1-[1-(allyloxycarbonyl)-1-(triphenylphosphoranyliden)methyl]-3-[1(R)hydroxyethyl]-4-(methoxyacetylthio)-azetidin--2-one as yellowish foam (4.2 g). The above prepared product was dissolved in toluene (250 ml) and refluxed 2 h. The residue, after cooling and removal of the solvent was purified through silica gel chromatography (cyclohexane-ethyl acetate mixtures as eluants) affording the title product as white powder (2.5 g). IR (KBr)ν 1775, 1705 cm⁻¹ UV (EtOH) λ max 326 nm Reference Example (5R,6S)-6-[1(R)-Hydroxyethyl]-2-Methoxymethyl-Penem-3-Carboxylic Acid Sodium Salt Allyl (5R,6S)-6-[1(R)hydroxyethyl]-2-methoxymethylpenem-3-carboxylate (2.5 g), dissolved in dry tetrahydrofuran (60 ml), was sequentially treated with sodium ethyl hexanoate (1.05 g),triphenylphosphine (300 mg) and tetrakis (triphenylphosphine) palladium (O) (100 mg). Stirring was continued for 30', after which time TLC monitoring showed no more starting material was left. Diethyl ether (40 ml) was added and the precipitate was isolated by centrifugation. The crude material was dissolved in the minimum amount of water and purified by reverse-phase chromatography ( LiChroprep® RP C-18 Merck; water then water-acetone as eluants) the product containing fractions were collected and freeze-dried to afford the title product as white powder (1.8 g). IR (KBr)ν 1755, 1600, 1575 cm⁻¹ UV (H₂O) λ max 258 nm (ε=4044); λ max 306 nm (ε=6076) NMR (200 MHz, D₂O)δ : 1.30 (3H,d, J=6.3 Hz) 3.38 (3H,s) 3.91 (1H,dd, J=1.6 and 6.0 Hz) 4.25 (1H,m) 4.48 and 4.79 (2H, two d, J=14.0 Hz) 5.66 (1H,d, J=1.6 Hz) Example 1 Acetoxymethyl (5R,6S)-6-[1(R)-Hydroxyethyl]-2-Methoxymethyl-Penem-3-Carboxylate (5R,6S)-6-[1(R)hydroxyethyl]-2-methoxymethylpenem-3-carboxylic acid sodium salt (258 mg) in dry DMF (3 ml) was treated with acetoxymethylbromide (145 mg) at 0°C, then stirred 2 h at r.t. After partitioning between AcOEt and 2% aqueous NaHCO₃, the organic phase was washed twice with brine then dried and concentrated in vacuo. Addition of diisopropyl ether to the crude product gave a white precipitate which was filtered and dried (220 mg). IR (KBr)ν 3590, 1780, 1765, 1715, 1580 cm⁻¹ UV (CHCl₃) λ max 327 nm NMR (CDCl₃, 90 MHz)δ : 1.33 (3H,d, J=6.5 Hz) 2.12 (3H,s) 2.3 (1H, bs, exch D₂O) 3.39 (3H,s) 3.71 (1H,dd, J= < 2 and 6.5 Hz) 4.17 (1H,m) 4.47 and 4.73 (2H, two d,J=16 Hz) 5.58 (1H,d J < 2 Hz) 5.82 (2H, center of ABq) Example 2 (5-Methyl-2-Oxo-1,3-Dioxolen-4-yl)Methyl (5R,6S)-6-[1(R)-Hydroxyethyl]-2-Methoxymethyl-Penem-3-Carboxylate A solution of (5R,6S)-6-[1(R)-hydroxyethyl]-2-methoxymethylpenem-3-carboxylic acid sodium salt (258 mg) in dry DMF (3 ml) was treated with (5-methyl-2-oxo--1,3-dioxolen-4-yl)methylbromide (180 mg) and stirred at r.t. for 2 h. The reaction mixture was poured into ethyl acetate/water, the organic phase was washed twice with water then dried and concentrated in vacuo. Treatment of the residue with diisopropyl ether gave white crystals (240 mg) UV (CHCl₃) λ max 326 nm IR (IR)ν 3450, 1820, 1780, 1725, 1710 cm⁻¹ NMR (CDCl₃, 90 MHz)δ : 1.32 (3H,d, 6.5 Hz) 2.17 (3H,S) 2.37 (1H, bs, exch. D₂O) 3.38 (3H,S) 3.69 (1H,dd, < 2 and 6.5 Hz) 4.20 (1H,m) 4.43 and 4.70 (2H, two d, J=16 Hz) 4.93 (2H,S) 5.60 (1H,d, J < 2 Hz) Example 3 (5-Methyl-2-Oxo-1,3-Dioxolen-4-yl)Methyl (5R,6S)-6-[1(R)-Hydroxyethyl]-2-Methoxymethyl-Penem-3-Carboxylate Step A To a stirred solution of (3S,4R)-3-[1(R)trimethylsilyloxyethyl]-4-methoxyacetylthio azetidin-2-one (3.3 g) in dry toluene (35 ml) at 10°C, triethylamine (1.8 ml) was added, followed by dropwise addition of (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyloxalylchloride (2.7 g) in toluene (10 ml). The resulting solution was stirred 15' at r.t., then washed with water, 5% aqueous sodium hydrogen carbonate and water again. After drying over Na₂SO₄ the organic solution was concentrated to a volume of 20 ml. Triethylphosphite (4 ml) was added and the solution was refluxed for 5 h. The mixture was cooled to r.t. then washed three times with water then dried over sodium sulphate. Removal of the solvent and chromatography of the residue over silica gel (n.hexane-ethyl acetate as eluants) gave (5-methyl-2-oxo-1,3--dioxolen-4-yl)methyl (5R,6S)-6-[1(R)-trimethylsilyloxyethyl]-2-methoxymethyl-penem-3-carboxylate as a colourless oil (2.9 g). Step B The above said product was dissolved in 95% ethanol (160 ml) and acetic acid (2 ml) was added. After stirring 1 h at r.t. the mixture was concentrated under vacuum to dryness. Addition of diisopropyl ether (50 ml) gave white cristals, which were filtered off and dried (2.0 g). UV (CHCl₃) λ max 326 nm IR (KBr)ν 3450, 1820, 1780, 1725, 1710, 1580 cm⁻¹

Claims: Claims for the following Contracting States : BE, DE, NL, SE A substantially (≧95%) optically pure (5R, 6S, 1'R) penem ester prodrug of the formula: wherein R is a) acyloxymethyl or 1-(acyloxy)ethyl; b) benzoyloxymethyl or 1-(benzoyloxy)ethyl, either unsubstituted or ring-substituted by a free, methylated or acetylated hydroxy or amino group; c) alkoxycarbonyloxymethyl or 1-(alkoxycarbonyloxy)ethyl; d) 3-phthalidyl; e) 2-oxo-1,3-dioxolan-4-yl, optionally substituted by a C₁-C₄ alkyl group in the 5 position; f) (2-oxo-1,3-dioxolen-4-yl)methyl, optionally substituted by a phenyl or C₁-C₄ alkyl group at the 5 position; g) a group CH₂CO₂R', wherein R' is C₁-C₄ straight or branched alkyl, or benzyl; or h) 2-oxotetrahydrofuran-5-yl, optionally substituted by a C₁-C₄ alkyl group at the 4 position. A compound according to claim 1 wherein R is pivaloyloxymethyl, propionyloxymethyl, cyclohexylacetoxymethyl, cyclohexanecarboxymethyl, dipropylacetoxymethyl, 1-(acetoxy)ethyl, 1-(cyclohexylacetoxy)ethyl, 1-(1-acetylsalicyloxy)ethyl, methoxycarbonyloxymethyl, ethoxycarbonyloxymethyl, isopropoxycarbonyloxymethyl, cyclohexylmethoxy-carbonyloxymethyl, 1-(methoxycarbonyloxy)-ethyl, 1-(ethoxycarbonyloxy)ethyl, 1-(bornyloxycarbonyloxy)ethyl, 3-phthalidyl, 2-oxo-1,3-dioxolan-4-yl, (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyl, ethoxycarbonylmethyl, tert-butoxycarbonylmethyl, (5-phenyl-2-oxo-1,3-dioxolen-4-yl)methyl,(2-oxo-1,3-dioxolen-4-yl-methyl or 2-oxotetrahydrofuran-5-yl. A compound according to claim 1, which is acetoxymethyl 6-(1-hydroxyethyl)-2-methoxymethyl-penem-3-carboxylate or (2-oxo-1,3-dioxolen-4-yl)methyl 6-(1-hydroxyethyl)-2-methoxymethylpenem-3-carboxylate. A process for the preparation of a substantially (≧95%) optically pure (5R, 6S, 1'R)-penem ester prodrug of formula (II) as defined in claim 1, which process comprises i) cyclising a compound of formula (III) wherein P' is either hydrogen or P'' where P'' is a hydroxy protecting group and R² is either R as defined in claim 1 or a carboxy protecting group; or ii) cyclising a compound of formula (IV) wherein P' and R² are as defined above, and Y is either oxygen or sulfur; and iii) if necessary, removing the optional protecting groups P'' and R₂ from the resultant compound, and converting the resulting compound of formula (I) or salt thereof into an ester prodrug of formula (II) by treatment with a compound of formula (V): R-X (V) wherein R is as defined in claim 1 and X is either a chloro, bromo or iodo atom or a mesyloxy, tosyloxy or trifluoro-methanesulphonyloxy group. A pharmaceutical composition comprising a compound according to claim 1 as active ingredient and a pharmaceutically acceptable carrier or diluent. A compound according to claim 1 for use as an antibacterial agent. Claims for the following Contracting State : GR A process for the preparation Of a substantially (≧95%) optically pure (5R, 6S, 1'R) penem ester prodrug of the formula: wherein R is a) acyloxymethyl or 1-(acyloxy)ethyl; b) benzoyloxymethyl or 1-(benzoyloxy)ethyl, either unsubstituted or ring-substituted by a free, methylated or acetylated hydroxy or amino group; c) alkoxycarbonyloxymethyl or 1-(alkoxycarbonyloxy)ethyl; d) 3-phthalidyl; e) 2-oxo-1,3-dioxolan-4-yl, optionally substituted by a C₁-C₄ alkyl group in the 5 position; f) (2-oxo-1,3-dioxolen-4-yl)methyl, optionally substituted by a phenyl or C₁-C₄ alkyl group at the 5 position; g) a group CH₂CO₂R', wherein R' is C₁-C₄ straight or branched alkyl, or benzyl; or h) 2-oxotetrahydrofuran-5-yl, optionally substituted by a C₁-C₄ alkyl group at the 4 position, which process comprises i) cyclising a compound of formula (III) wherein P' is either hydrogen or P'' where P'' is a hydroxy protecting group and R² is either R as defined above or a carboxy protecting group; or ii) cyclising a compound of formula (IV) wherein P' and R² are as defined above, and Y is either oxygen or sulfur; and iii) if necessary, removing the optional protecting groups P'' and R² from the resultant compound, and converting the resulting compound of formula (I) or salt thereof into an ester prodrug of formula (II) by treatment with a compound of formula (V): R-X (V) wherein R is as defined above and X is either a chloro, bromo or iodo atom or a mesyloxy, tosyloxy or trifluoro-methanesulphonyloxy group. A process according to claim 1 wherein R is pivaloyloxymethyl, propiony-loxymethyl, cyclohexylacetoxymethyl, cyclohexanecarboxy-methyl, dipropylacetoxymethyl, 1-(acetoxy)ethyl, 1-(cyclohexylacetoxy)ethyl, 1-(1-acetylsalicyloxy)ethyl, methoxycarbonyloxymethyl, ethoxycarbonyloxymethyl, isopropoxycarbonyloxymethyl, cyclohexylmethoxy-carbonyloxymethyl, 1-(methoxycarbonyloxy)ethyl, 1-(ethoxy-carbonyloxy)ethyl, 1-(bornyloxycarbonyloxy)ethyl, 3-phthalidyl, 2-oxo-1,3-dioxolan-4-yl, (5-methyl-2-oxo-1,3-dioxolen-4-yl)methyl, ethoxycarbonylmethyl, tert-butoxycarbonylmethyl, (5-phenyl-2-oxo-1,3-dioxolen-4-yl)methyl, (2-oxo-1,3-dioxolen-4-yl-methyl or 2-oxotetrahydrofuran-5-yl. A process according to any one of the preceding claims wherein the resulting compound of formula (II) is formulated into a pharmaceutical composition.

Inventors: ALPEGIANI MARCO, DELLA BRUNA COSTANTINO, FRANCESCHI GIOVANNI, PERRONE ETTORE, ZARINI FRANCO, ALPEGIANI, MARCO, DELLA BRUNA, COSTANTINO, FRANCESCHI, GIOVANNI, PERRONE, ETTORE, ZARINI, FRANCO
Applicants: ERBA CARLO SPA, FARMITALIA CARLO ERBA S.R.L.
Application year: 1988
Main classification: C07D499
IPC codes: A61P31, C07D499, A61K31