(1R,5S,6S)-2-(substituted thio)-6-[(R)-1-hydroxy-ethyl]-1-methyl-carbapenem-3-carboxylic-acid derivatives

Abstract: Novel carbapenem compounds, (1R,5S,6S)-2-­ substituted thio-6-[(R)-1-hydroxyethyl]-1-methyl-­ carbapenem-3-carboxylic acid derivatives. These carbapenem compounds are represented by the following formula having a beta-coordinated methyl group introduced into the 1-position and a 4-pyrazol­ izinylthio group or a 6,7-dihydro-5H-pyrazolo[1,2-a]­ [1,2,4]triazolium-6-ylthio group introduced into the 2-position. In the formula, R¹ represents a radical of the formula and R² is a hydrogen atom or an anion charge. They have antibacterial activity, and are used for pre­ vention and treatment of bacterial infections as anti­ bacterial agents. A process for producing these carba­ penem compounds is also provided.

Description: BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a carbapenem antibiotic and, more particularly, to a 1β-methyl-carba­ penem derivative having a methyl group introduced at the 1-position of the carbapenem skeleton to an antibacterial composition containing the same as an active ingredient, and to a process of preparing the same. 2. Description of the Prior Art Heretofore, as various antibacterial sub­ stances, there have been proposed many carbapenem anti­ biotic substances having, as a basic skeleton, carba-2-­ penem-3-carboxylic acid represented by the following formula (A): For example, an initial generation of carba­ penem antibiotics is a naturally occurring carbapenem compound such as thienamycin represented by the formula (B): The thienamycin may be obtained from a fermen­ tation broth of Streptomyces cattleya and has a broad range of antibacterial spectra against Gram-positive and Gram-negative bacteria. It has been expected therefore to be developed as a highly useful compound, but its poor chemical stability has precluded its commercialization. With the foregoing background, many re­ searchers have attempted to develop a carbapenem compound having antibacterial activities as high as thienamycin and ensuring more chemical stability. As a result, there has been developed imipenem (INN) represented by the following formula (C): This compound is a practically available antibacterial agent and may be obtained by converting an amino group as a side chain at the 2-position to a formimidoyl group. The imipenem of the formula (C) exhibits anti­ bacterial activities higher than those of the thienamycin and ensures some degree of chemical stability; however, it presents the disadvantage that it is decomposed within a short period of time by kidney dehydropeptidase (DHP) in the living body. For this reason, it cannot be ad­ ministered singly, and must be used in combination with a DHP inhibitor in order to control its decomposition leading to inactivation. Its formulation for clinical administration is a combination with cilastatin (INN) that is a DHP inhibitor. An antibacterial agent preferred for practical clinical use, however, is one that alone can demonstrate antibacterial activity. Furthermore, the DHP inhibitor to be combined with the antibiotic could exert undesir­ able actions on tissues of the living body. For these reasons, the combined use should be avoided wherever possible. Thus there has been a growing demand for a carbapenem compound having sufficiently high degrees of both antibacterial activity and resistance to DHP. Recently, there were proposed some carbapenem compounds of the type that could achieve the above objectives. Such carbapenem compounds are 1-methyl-­ carbapenem compounds in which a methyl group is in­ troduced at the 1-position of the carbapenem skeleton. Most recently, another type of carbapenem compounds was proposed which has a heterocycloalkylthio group at the 2-position of the carbapenem skeleton. For example, European Patent Publication No. 170,173 (Japanese Laid-­ Open Patent Publication No. 83,183/1986) to Merck dis­ closes 1-methylcarbapenem compounds having at the 2-­ position an alkylated mono- or bi-cyclic quaternary heteroaryl alkylthio substituent, represented by the formula (D): It is reported that these compounds have superior antibacterial activities as well as a remarkably improved resistance to decomposition by DHP leading to inactiva­ tion so that they demonstrate highly useful effects. It should be noted here that the Japanese Patent Laid-Open Publication No. 83,183/1986 discloses merely a general concept of 1β-methyl-carbapenem com­ pounds and a very limited number of specific working examples. It also discloses in generic terms that they are superior in antibacterial activities; however, it does not specifically describe any antibacterial data whatsoever. Furthermore, the Japanese patent document merely enumerates more than about 470 compounds by their names only; but it contains as slightly less than 10 compounds that have been actually supported by working examples. No specific compounds according to the present invention are disclosed therein and the prior patent application quoted above does not hint anything about such compounds having superior pharmacological charac­ teristics as demonstrated and claimed by the present invention. Furthermore, U. S. Patent 4,644,061 assigned to Bristol Myers discloses carbapenem compounds with a quaternary heteroalkylthio substituent at the 2-position of the carbapenem skeleton, as represented by the formula (E): wherein R¹ is a hydroxyethyl group; R⁸ is a hydrogen atom; and R¹⁵ is a hydrogen atom or a methyl group. However, since all of these patent applications contain very broad generic disclosures and none of them specifically name or describe the combination of the present invention, they are non-anticipatory of the selective invention disclosed and claimed herein. SUMMARY OF THE INVENTION The present invention provides carbapenem compounds having high antibacterial activities, a strong action of inhibiting β-lactamase as well as improved resistance to kidney dehydropeptidase. More specifical­ ly, the present invention provides the carbapenem com­ pounds substituted by a methyl group at the 1-position in the β-configuration, in which particularly a 4-pyrazol­ idinyl thio group or a pyrazolotriazolinium-6-yl thio group is introduced at the 2-position. Specifically, the present invention provides (1R,5S,6S)-2-substituted thio-6-[(R)-1-hydroxyethyl]-­ 1-methyl-carbapenem-3-carboxylic acid derivatives re­ presented by formula (I): wherein R¹ represents a radical of the formula and R² is a hydrogen atom or an anion charge, or a pharmaceutically acceptable salt thereof. The present invention further provides an antibacterial composition containing the carbapenem compound represented by formula (I) or a pharmaceutically acceptable salt thereof, as an active ingredient. For the above purpose of the invention, preferred carbapenem compounds of formula (I) are (1R,5S,6S)-2-[4-pyrazolidinyl]thio-6-[(R)-1-hydroxy­ ethyl]-1-methyl-carbapenem-3-carboxylic acid as formula (I-1): or a pharmaceutically acceptable salt thereof, and (1R,5S,6S)-2-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]­ triazolium-6-yl)]thio-6-[(R)-1-hydroxyethyl]-1-­ methyl-carbapenem-3-carboxylate as formula (I-2): or a pharmaceutically acceptable salt thereof. Furthermore, the present invention provides a crystalline carbapenem compound of the formula (I-2) or a pharmaceutically acceptable salt thereof. DETAILED DESCRIPTION OF THE INVENTION The carbapenem compounds according to the present invention are novel compounds that are not specifically disclosed in the prior patent publications (for instance, Japanese Patent Laid-Open Publication No. 83,183/1986). In particular, they are remarkably charac­ terized in that the substituent at the 2-position of the carbapenem skeleton is a (5H-pyrazolo[1,2-a]triazolium-­ 6-yl)thio group and in that they have superior anti­ bacterial activities and resistance to DHP. In accordance with the present invention, the carbapenem compounds represented by formula (I) may be prepared basically by a process to be described below. Briefly stated, the carbapenem of formula (I) may be prepared by reacting a compound represented by formula (II): wherein R³ is a carboxyl protecting group, and R a is an acyl group, with a mercapto reagent represented by formula (III): wherein R b is an amino protecting group, to give a compound represented by formula (IV): wherein R³ and R b have the same meanings as above, and subjecting the compound of the formula (IV) to re­ moval of the protecting groups R³ and R b to give the carbapenem compound of the formula (I-1) and then, if desire, reacting the resulting compound of formula (I-1) with formimidic acid ester to give the carbapenem compound represented by formula (I-2) More specifically, the carbapenem compounds represented by formula (I) may be prepared in such a manner as will be described in detail below. The carbapenem compound represented by formula (II) to be employed as a starting compound in the process described above is known per se and may be prepared in such a manner as disclosed, for example, in U. S. Patent 4,312,871 (Japanese Patent Laid-Open Publication No. 123,985/1981) or, more preferably, in accordance with the spatially selective method as indicated in Reaction Scheme A below and proposed by the present inventors (as disclosed, for example, in Japanese Patent Application No. 315,444/1986). wherein R⁴ is a hydrogen atom or a lower alkyl group; Z is tertiary-butyldimethylsilyl group; and R³ and R a have the same meanings as above. In the specification of the present applica­ tion, the term "lower" qualifying a group of a compound means that the group or compound so qualified has from 1 to 7, preferably from 1 to 4, carbon atoms. The term "lower alkyl" referred to herein stands for a straight-chained or branched-chain hydro­ carbon group having preferably from 1 to 6 carbon atoms and may include, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert.-butyl, n-pentyl, isopentyl, n-hexyl, isohexyl or the like. The term "carboxyl protecting group" referred to herein stands for any group capable of protecting the carboxyl group of the compound involved without adversely affecting any other substituents and the reactions that follow and may include, for example, an ester residue such as a lower alkyl ester residue including, for ex­ ample, methyl ester, ethyl ester, n-propyl ester, iso­ propyl ester, n-, iso-, sec- or tert.-butyl ester, n-­ hexyl ester or the like; an aralkyl ester residue in­ cluding, for example, benzyl ester, p-nitrobenzyl ester, o-nitrobenzyl ester, p-methoxybenzyl ester or the like; and a lower aliphatic acyloxymethyl ester residue in­ cluding, for example, acetoxymethyl ester, propionyl­ oxymethyl ester, n- or iso-butyryloxymethyl ester, pivaloyloxymethyl ester or the like. The term "acyl group" referred to herein stands for, in a narrower sense, a moiety obtainable by removing the hydroxyl group from the carboxyl group of an organic carboxylic acid as well as, in a broader sense, any acyl group derived from an organic sulfonic acid or an organic phosphoric acid. Such an acyl group may include, for example, a lower alkanoyl group such as acetyl, propionyl, butyryl or the like, a (halo)lower alkyl sulfonyl group such as methanesyulfonyl, trifluoromethanesulfonyl or the like; a substituted or unsubstituted arylsulfonyl group such as benzenesulfonyl, p-nitrobenzenesulfonyl, p-bromo­ benzenesulfonyl, toluenesulfonyl, 2,4,6-triisopropyl­ benzenesulfonyl or the like; and diphenylphosphoryl. The term "amino protecting group" referred to herein stands for groups usually employed in peptide chemistry, for example, phthaloyl, benzyloxycarbonyl, tert.-butoxycarbonyl, p-nitrobenzyloxycarbonyl or the like. When R² in formula (I) represents an anion charge, -COOR² bonded to the 3-position of the carbapenem skeleton is -COO ⊖ , and -COO ⊖ forms an intramolecular salt with represented by R¹. Each of the steps of the Reaction Scheme A above for preparing the compounds represented by formula (II) in a highly spatial selectivity will be described below more in detail. The step (a) involves the reaction of the N-propionyl-1,3-thiazoline-2-thione derivative of formula (VI) with a tin(II) triflate in the presence of a base to give an enolate and then the reaction of the resulting enolate with the compound of formula (V) to give the azetidin-2-one derivative of formula (VII). The enolization reaction of the N-propionyl-­ 1,3-thiazoline-2-thione derivative of formula (VI) with the tin(II) triflate may be carried out usually in a solvent inert in the reaction, such as an ether, i.e., diethyl ether, tetrahydrofuran or the like; a hydro­ carbon, i.e., toluene, cyclohexane or the like; a halogenated hydrocarbon, i.e., dichloromethane, chloro­ form or the like. Preferably tetrahydrofuran can be used. The reaction temperature is not limited to a particular range of temperatures and may vary in a wide range with starting materials to be used or the like. Usually the reaction temperature may be in a range of relatively low temperatures as low as from approximately -100°C to about room temperature, preferably from ap­ proximately from -78°C to approximately 0°C. The quantity of the tin(II) triflate with respect to the compound of formula (VI) is not critical and may range usually from approximately 1 mole to ap­ proximately 2 moles, preferably from 1 to 1.5 moles, per mole of the compound of formula (VI). The enolization reaction above is carried out usually in the presence of the base including, for ex­ ample, a tertiary amine such as triethylamine, diiso­ propylethyl amine, 1,4-diazabicyclo[2.2.2]octane, N-­ methylmorpholine, N-ethylpiperidine, pyridine or the like. N-Ethylpiperidine is employed advantageously. The base may be used at a rate ranging generally from approximately 1.0 to approximately 3 molar equivalents, preferably from 1.0 to 2 molar equivalents, per mole of the compound of formula (VI). The enolization reaction as described above may be completed generally in approximately 5 minutes to approximately 4 hours, thus leading to the formation of the enolate. After completion of the enolization reaction, the resulting enolate may be used as it is for further reaction with the compound of formula (V). The resulting enolate is then subjected to the alkylation reaction with the compound of formula (V). The alkylation reaction may be conducted at temperatures in the range generally from approximately -100°C to about room temperature, preferably from approximately -78°C to approximately 10°C. The quantity of the compound of formula (V) is not critical and may vary conveniently in a range generally from approximately 0.5 mole to approxi­ mately 5 moles, preferably from 0.5 to 2 moles, per mole of the compound of formula (VI) used for the enolization. The alkylation reaction may be carried out under such conditions as described above generally for approximately 5 minutes to approximately 5 hours, pre­ ferably for 5 minutes to approximately 2 hours. The enolization and alkylation reactions may be carried out preferably in an inert atmosphere such as an atmosphere of nitrogen gas or argon gas. The reaction product obtained by the above reaction is then treated with water. For instance, after completion of the reaction, a phosphate buffer with ap­ proximately pH 7 is added, and a mixture is then stirred to be followed by filtration of undissolved materials. After filtration, the compound of the formula (VII) is separated and purified in conventional manner, such as by means of extraction, recrystallization and chromato­ graphy. The step (b) is a step by which the compound of formula (VIII) may be prepared by reacting the azetidin-­ 2-one derivative of formula (VII) obtained by the step (a) above with a magnesium malonate represented by the general formula: R³OOCCH₂CO₂)₂Mg in the presence of imidazole. The reaction is carried out preferably in an inert organic solvent such as an ether solvent, i.e., ether, tetrahydrofuran or dioxane; a hydrocarbon solvent, i.e., toluene, xylene or cyclohexane; a halogenated hydrocarbon solvent, i.e., dichloromethane or chloroform; and acetonitrile. Particularly acetonitrile may be employed conveniently. The reaction temperature is not limited strictly to a particular range and may vary in a wide range with starting materials to be used or the like. They may range generally from approximately 0°C to ap­ proximately 100°C, preferably around room temperature. The quantity of the magnesium malonate with respect to the compound of formula (VII) may be about an equimolar amount, and the reaction may be completed in approximately 50 hours, preferably in approximately 20 hours. The magnesium malonate to be used may include, for example, p-nitrobenzylmagnesium malonate, benzyl­ magnesium malonate, methylmagnesium malonate and so on. Among them, p-nitrobenzylmagnesium malonate is preferably used. The step (c) is a step to eliminate a hydroxyl protecting group Z from the compound of formula (VIII) obtained by the step (b) above. The tertiary-butyl-­ dimethylsilyl group as the hydroxyl protecting group Z may be eliminated by subjecting the compound of formula (VIII) to acidic hydrolysis in a solvent such as methanol, ethanol, tetrahydrofuran, dioxane or the like in the presence of an acid such as a mineral acid, i.e., hydro­ chloric acid, sulfuric acid or an organic acid, i.e., acetic acid at temperatures ranging from 0°C to 100°C for reaction periods ranging from 0.5 to 18 hours. The above step may yield the compound re­ presented by formula (IX) in a quantitative amount. The step (d) is a step by which the diazo compound of formula (X) may be prepared by treating the compound of formula (IX) obtainable by the above step (c) with an azide compound in the presence of a base in such an inert organic solvent as have been enumerated for the step (d) above. The azide compound to be used in the step (d) may include, for example, p-carboxylbenzenesulfonyl azide, toluenesulfonyl azide, methanesulfonyl azide, dodecylbenzenesulfonyl azide or the like. The base to be used therein may include, for example, triethylamine, pyridine, diethylamine or the like. The reaction may be carried out, for instance, by adding p-toluenesulfonyl azide to acetonitrile pre­ ferably in the presence of triethylamine at 0°C to 100°C, preferably at room temperature for 1 to 50 hours. This reaction produces the diazo compound represented by formula (X) in a high yield. The step (e) is a step by which the diazo compound of the formula (X) obtainable by the step (d) above may be cyclized to give the compound of formula (XI). This step may be carried out preferably in an inert solvent such as benzene, toluene, tetrahydrofuran, cyclohexane, ethyl acetate, dichloromethane or the like, preferably in toluene, at temperatures ranging from 25°C to 110°C for 1 to 5 hours in the presence of a metal catalyst such as a metal carboxylate compound including, for example, bis(acetylacetonate)Cu(II), CuSO₄, copper powder, Rh₂(OCOCH₃)₄, rhodium octanoate, Pb(OCOCH₃)₄ or the like. As an alternative procedure, the above cyclization step may be carried out by subjecting the compound of formula (X) to irradiation from a light source through a Pyrex filter (its wavelength being larger than 300 nm) in a solvent such as benzene, diethyl ether or the like at 0°C to 250°C for 0.5 to 2 hours. The step (f) produces the compound of formula (II) by reacting the compound of formula (XI) obtainable by the step (e) with a reactive derivative of an acid represented by the formula: R a OH. The reactive acid derivative may include, for example, an acid anhydride such as acetic acid anhydride, methanesulfonic acid anhydride, p-toluenesulfonic acid and anhydride, p-nitro­ benzenesulfonic acid anhydride, 2,4,6-triisopropyl­ benzenesulfonic acid anhydride, trifluoromethanesulfonic acid anhydride or the like or an acid halide such as an acid chloride, i.e., acetyl chloride, propionyl chloride, diphenylphosphoric chloride, toluenesulfonyl chloride, p-bromobenzenesulfonyl chloride or the like. Diphenylphosphoric chloride (R a = diphenylphosphoryl group) is particularly preferred. The reaction of the compound of formula (XI) with the reactive acid derivative may be carried out, for example, in a manner similar to a conventional acylation reaction in an inert solvent such as methylene chloride, acetonitrile, dimethylformamide or the like, conveniently in the presence of a base such as diisopropylethyl amine, triethylamine, 4-dimethylaminopyridine or the like at temperatures ranging from -20°C to 40°C for approximately 30 minutes to approximately 24 hours. The reaction consisting of a series of the steps as have been described above provides the compound represented by formula (II) with a highly spatial selec­ tivity and with such a spatial arrangement that the methyl group at the 1-position of the carbapenem skeleton is arranged in the R configuration, the substituent at the 5-position thereof is in the R configuration, and the substituent and the hydroxymethyl group each at the 6-position thereof are in the S and R configurations, respectively. The compound represented by formula (II) is then reacted with a mercapto reagent represented by formula (III) to give the compound represented by formula (IV). The reaction of the compound of formula (II) with the mercapto reagent of formula (III) may be carried out, for instance, by reacting the compound of formula (II) with the mercapto reagent of formula (III) in an excess amount ranging from about an equimolar amount to approximately 1.5 molar amount in an appropriate solvent such as tetrahydrofuran, dichloromethane, dioxane, di­ methylformamide, dimethylsulfoxide; acetonitrile, hexa­ methylene phosphoramide or the like, preferably in the presence of a base such as sodium hydrogen carbonate, potassium carbonate, triethylamine, diisopropylethyl amine or the like at a temperature range from approx­ imately -40°C to approximately 25°C for approximately 30 minutes to approximately 24 hours. The reaction described above provides the carbapenem compound represented by formula (IV) in which the carboxyl group at the 3-position thereof is protected by the carboxyl protecting group R³ and the substituent at 2-position thereof is protected by the amino protect­ ing group R b . The removal of the protecting groups R³ and R b may be made by a reaction known per se for re­ moving a protective group, such as solvolysis or hydro­ genolysis. In a typical reaction, the compound re­ presented by formula (IV) may be treated, for instance, in a mixture of solvents such as tetrahydrofuran-water, tetrahydrofuran-ethanol-water, dioxane-water, dioxane-­ ethanol-water, n-butanol-water or the like containing morpholino-propane sulfonic acid-sodium hydroxide buffer solution (pH 7), a phosphate buffer solution (pH 7), dipotassium phosphate, sodium bicarbonate or the like, using hydrogen under 1 to 4 atmospheric pressures, in the presence of a catalyst for hydrogenation such as platinum oxide, palladium-activated carbon or palladium hydroxide-­ activated carbon at temperatures ranging from approx­ imately 0°C to approximately 50°C for approximately 0.25 to approximately 4 hours. As a result, (1R,5S,6S)-2-[4-pyrazolidinyl]­ thio-6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-3-­ carboxylic acid represented by formula (I-1), one of the desired compounds of the invention, is produced. By reacting the resulting compound of formula (I-1) with a formimidic acid ester derivative such as ethyl formimidate hydrochloride, methyl formimidate hydrochloride or benzyl formimidate hydrochloride under weakly basis conditions (for example, in a reaction medium adjusted to a pH of about 8.5 with a phosphate buffer having a pH of 7.0 and a 1N aqueous solution of sodium hydroxide), (1R,5S,6S)-2-[(6,7-dihydro-5H-­ pyrazolo[1,2-a][1,2,4]triazolium-6-yl)]thio-6-[(R)-1-­ hydroxyethyl]-1-methyl-carbapenem-3-carboxylate of formula (I-2), another desired compound of the invention. One presumed course of reaction in the for­ mation of the compound of formula (I-2) by the reaction of the compound of formula (I-1) with the formimidic acid ester derivative is that by the reaction of the compound (I-1) with the formimidic acid ester derivative, (1R,5S,6S)-6-[(R)-1-hydroxyethyl]-1-methyl-2-[(1,2-di­ iminomethyl)-4-pyrazolidinyl]thiocarbapenem-3-carboxylic acid of the following formula is formed as an intermediate, and this intermediate undergoes cyclization reaction to form the compound of formula (I-2). In the above reaction, the mercapto reagent of formula (III) is a novel compound not described in the prior literature. It can be obtained, for example, in accordance with the following Reaction Scheme B. wherein R b has the same meaning as above, X¹ and X² are halogen atoms such as a chlorine atom; Ms is methanesulfonyl groups; and R c is a lower acyl group such as acetyl, propionyl, butyryl group. 4-Hydroxypyrazoline of formula (XII) prepared by the reaction between hydrazine hydrate and epihalo­ hydrin is treated with the acylation reagent of the formula R b X² to give the compound of formula (XIII). Then, the resulting compound of formula (XIII) is con­ verted to the compound of formula (XIV) by methane­ sulfonylation, and the compound of formula (XIV) is reacted with the compound of the formula R c SH such as thiolacetic acid to obtain the compound of formula (XV). Finally, the resulting compound of formula (XV) is con­ verted to the objective mercapto reagent of formula (III) by reacting with alkali metal alkoxide such as sodium methoxide, sodium ethoxide or the like. The compound of the present invention repre­ sented by formula (I-1) may be converted to a pharma­ ceutically acceptable salt thereof in a usually manner. Such a salt may be, for example, an alkali metal salt such as sodium, potassium salt thereof; an amino acid salt such as arginine, ornithine, lysine salt thereof; and an ammonium salt such as diethanolammonium, tri­ ethanolammonium salt thereof, but the sodium or potassium salt thereof may be more preferable. In actual application, the (1R,5S,6S)-2-­ [(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazolium-6-yl)]­ thio-6-[(R)]1]hydroxyethyl]-1-methyl-carbapenem-3-carb­ oxylate of formula (I-2) may be converted to a preferred crystalline compound. This crystallization may be carried out by treating the compound of formula (I-2) preferably in water or in an aqueous medium such as water-alcohol, and the resulting crystals may be in the form of an anhydrous compound or a hydrate. The desired compounds of formula (I) in accord­ ance with the present invention are novel compounds that are not disclosed specifically in the above-mentioned publication and that are extremely stable against de­ hydropeptidase (DHP) known as a kidney enzyme and have superior antibacterial activities. The remarkably high antibacterial activities and stability against the kidney DHP of the compounds of formula (I) according to the present invention have been determined by biological tests described below. I . Antibacterial Tests Test Procedures : The antibacterial activities were tested by an agar plate dilution method in accordance with the standard method of The Japanese Chemotherapy Society [Chemotherapy, Vol. 29, 76-79 (1981)]. A Mueller-Hinton (MH) agar liquid medium of a test microorganism was cultured overnight at 37°C and the resultant culture medium was diluted with a buffered saline gelatin (BSG) solution to contain approximately 10⁶ cells of the test microorganisms per milliliter, and then the diluted solution was inoculated with a micro­ planter at the rate of approximately 5 microliters on a MH agar medium containing a test compound. This medium was then incubated at 37°C for 18 hours. The minimum in­ hibitory concentration (MIC) is determined as a minimum concentration in which no test microorganism could grow. It is noted here that the test organisms used were all standard strains. Results : Table 1 shows the test results. The test compound used therein was the compound (15) obtained in Example 6. As control compounds were used ones clinically employed widely, viz., cefazolin (CEZ) as a cephalosporin compound, and imipenem as a carbapenem compound. The foregoing results clearly demonstrate that the carbapenem compounds according to the present inven­ tion have superior antibacterial activities. II . Antibacterial Activities against Clinically Isolated β-Lactamase (Cephalosporinase) Producing Strains Test Procedures : The antibacterial activities agaisnt clinically isolated β-lactamase producing strains were tested by the agar plate dilution method in accordance with the standard method of The Japanese Chemotherapy Society [Chemotherapy, Vol. 29, 76-79 (1981)]. A solution of a cephalosporinase producing strain stored by Episome Research Institute, prepared by incubating the strain in a sensitivity test broth (STB; product of Nissui K. K.) for 18 hours, was diluted with a fresh STB solution so that the solution contained 10⁶ cells per milliliter. The diluted solution was then inoculated as spots in a microplanter on a sensitivity disk agar-N (SDA; product of Nissui K. K.) containing a test compound. The disk agar was then incubated for 18 to 20 hours. The minimum inhibitory concentration was determined as a minimum concentration in which the test microorganism no longer grew after a 18 to 20-hour incubation. Results : Table 2 shows the test results. The test compound used therein was the compound (15) obtained in Example 6. As control compounds, there were used ceftazidime (CAZ) as a cephalosporin compound, and imipenem as a carbapenem compound, both being recognized as having remarkably high antibacterial activities against the test strains and being widely employed clinically. The above results show that the carbapenem compounds according to the present invention had the antibacterial activities against P. aeruginosa and P. cepacia belonging to Pseudomonadaceae as high as imipenem and particularly higher than CAZ having anti-Pseudomonas activities. It has been further found that the carbapenem compounds of the invention had activities against enteric bacteria excluding the genus Proteus as high as imipenem and superior to CAZ. III . Sensitivity Tests against Clinical Isolates 1. P. aeruginosa resistant strains (1) Strains of Test Organisms: Fifty-four strains of P. aeruginosa demonstrat­ ing resistance against the following agents in the con­ centrations indicated in the following parentheses were employed for sensitivity tests against clinical isolates. Ceftazidime (CAZ) (25 to 100 µg/ml) 21 strains Cefsulodine (CFS) (25 to >100 µg/ml) 23 strains Piperacilin (PIPC) (25 to >100 µg/ml) 15 strains Gentamycin (GM) (25 to >100 µg/ml) 21 strains Amikacin (AMK) (25 to >100 µg/ml) 26 strains Ofloxacin (OFLX) (25 to >100 µg/ml) 4 strains (2) Test Procedures: The test procedures were based on the agar plate dilution method in accordance with the standard method of The Japanese Chemotherapy Society. The minimum inhibitory concentration (MIC) was determined in sub­ stantially the same manner as the test procedures II above using the 54 strains of P. aeruginosa having an anti-Pseudomonas resistance. (3) Results: The compound (15) obtained in Example 6 was found to demonstrate antibacterial activities to inhibit the growth of approximately 98% of the test micro­ organisms in a concentration of 6.25 µg/ml and all the test microorganisms in a concentration of 12.5 µg/ml. The imipenem, on the other hand, was found to inhibit the growth of approximately 98% of the test microorganisms in a concentration of 6.25 µg/ml and all the test microorganism in a concentration of 12.5 µg/ml. 2. C. freundii resistant strains (1) Strains of the Test Organisms: Twenty-seven strains of C. freundii demonstrat­ ing a resistance against the following agent as same manner described above in Test 1. Cefixime (CFIX) (50 to >100 µg/ml) Cefotaxime (CTX) (50 to >100 µg/ml) (2) Test Procedures: The tests were carried out in the same manner as described above in Test 1. (3) Results: The compound (15) obtained in Example 6 was found to demonstrate antibacterial activities to inhibit the growth of approximately 98% of the test micro­ organisms in a concentration of 0.78 µg/ml and all the test microorganisms in a concentration of 1.56 µg/ml. The imipenem, on the other hand, was found to inhibit the growth of approximately 90% of the test microorganisms in a concentration of 0.78 µg/ml and all the test microorganisms in a concentration of 1.56 µg/ml. The above results clearly demonstrate that the compounds according to the present invention have superior antibacterial activities to imipenem. IV . Stability Test against Kidney Dehydropeptidase: 1. Materials : (1) Swine Kidney Dehydropeptidase-I (DHP-I): The swine kidney (8 kg) was homogenized and the enzyme protein was allowed to precipitate. After a con­ nective lipid was removed with acetone, the resultant material was made soluble by treatment with butanol and purified by the ammonium sulfate fraction method, thereby producing DHP-I enzyme from a 75% ammonium sulfate frac­ tion. The DHP-I enzyme was then adjusted to give an enzyme concentration of 25 mg/10 ml (phosphate buffer, pH 7.1), and divided into 1 ml portions. The portions were frozen and stored at -40°C or less until use. (2) Test Compound: Compound (15) obtained in Example 6 below was used as a test compound. The test compound was adjusted in situ to give a concentration of 117 µM with a 60 mM sodium phosphate buffer solution (pH =7.1). Glycyl dehydrophenylalanine (Gl-dh-Ph) and imipenem were employed as control compounds, and they were adjusted in situ each to give a concentration of 117 µM with the same sodium phosphate buffer solution. 2. Method : (1) Measurement for Hydrolysis Activity against DHP-I Enzyme Substrate by Late Assay: To 1.2 ml of 50 mM sodium phosphate buffer solution (substrate) containing 117 µM of each of Gl-dh-Ph and imipenem as the control compounds was added 0.2 ml of the DHP-I enzyme solution (25 mg/10 ml) prepared above in the final substrate concentration of 100 µM. The solution was then incubated at 37°C for 10 minutes. The initial velocity of hydrolysis of the substrate was measured from a decrease in absorbency at a particular λmax of each of the substrates. A blank test was conducted in substantially the same manner as above by adding 0.2 ml of the sodium phosphate buffer solution (pH 7.1) to 1.2 ml of the above substrate. (2) Measurement for Stability of Test Compounds against DHP-I by High Performance Liquid Chromatography Method (HPLC): The test compound according to the present invention and the control compounds were treated in substantially the same manner as (1) above. The in­ cubation, however, was conducted at 37°C for 4.5 hours or for 24 hours. The degree of hydrolysis of the compounds each after the test periods was measured by the HPLC method. 3. Results : The initial velocity of hydrolysis of each of the substrates against DHP-I by the late assay was found as follows: Gl-dh-Ph: 17.4 µM/minute Imipenem: 0.56 µM/minute Table 3 below shows measurement results on stability of the compound according to the present invention and imipenem against DHP-I. The stability test results against DHP-I clearly show that the carbapenem compound according to the present invention was approximately twenty-eight times as stable imipenem. V. Toxicity : Toxicological studies were carried out using a group of 10 male mice of CrjCD(SD) strain weighing from 20 to 23 grams. A solution containing the carbapenem compound (15) of the present invention obtained by Example 6 was administered subcutaneously to the mice and sub­ jected to observations for one week. The results have revealed that the group of mice to which the carbapenem compound (15) of the present invention had been administered in the amount of 500 mg/kg were alive without any abnormal observations. As described above, the carbapenem compounds according to the present invention demonstrate a wider scope of antibacterial spectra than do conventional cephalosporin compounds, and remarkable antibacterial activities comparable to imipenem as well as an over­ whelmingly higher resistance against DHP than imipenem. The carbapenem compounds according to the present invention further possess antibacterial activities against clinically isolated strains and present favorable effects on infection preventive tests on mice against various organisms. Therefore, the carbapenem compounds of formula (I) according to the present invention permit a single administration without combination with any other com­ pounds and without a risk of any side effect that might be caused in their combined use with a DHP inhibitor, unlike imipenem that was led for the first time to a practically useful antibacterial agent in combination with cilastatin acting as a DHP inhibitor. The carbapenem compounds are accordingly extremely useful as anti­ bacterial agents for therapy and prevention of infectious diseases from various pathogenic organisms. The carbapenem compound of formula (I) ac­ cording to the present invention may be administered as an antibacterial agent to the human being and other mammalian animals in the form of a pharmaceutically acceptable composition containing an antibacterially effective amount thereof. The administration dose may vary in a wide range with ages, patients, weights and conditions of patients, forms or routes of administra­ tion, physicians' diagnoses or the like and may be orally, parenterally or topically administered, to adult patients usually in a standard daily dose range from approximately 200 to approximately 3,000 mg once or in several installments per day. The pharmaceutically acceptable composition of the carbapenem compound of formula (I) according to the present invention may contain an inorganic or organic, solid or liquid carrier or diluent, which is conven­ tionally used for preparations of medicines, particularly antibiotic preparations, such as an excipient, e.g., starch, lactose, white sugar, crystalline cellulose, calcium hydrogen phosphate or the like; a binder, e.g., acacia, hydroxypropyl cellulose, alginic acid, gelatin, polyvinyl pyrrolidone or the like; a lubricant, e.g., stearic acid, magnesium stearate, calcium stearate, talc, hydrogenated plant oil or the like; a disintegrator, e.g., modified starch, calcium carboxymethyl cellulose, low substituted hydroxypropyl cellulose or the like; or a dissolution aid, e.g., a non-ionic surface active agent, an anionic surface active agent or the like, and may be prepared into forms suitable for oral, parenteral or topical administration. The formulations for oral ad­ ministration may include solid preparations such as tablets, coatings, capsules, troches, powders, fine powders, granules, dry syrups or the like or liquid preparations such as syrups or the like; the formulations for parenteral administration may include, for example, injectable solutions, drip-feed solutions, depositories or the like; and the formulations for topical administra­ tion may include, for example, ointments, tinctures, creams, gels or the like. These formulations may be formed by procedures known per se to those skilled in the art in the field of pharmaceutical formulations. The carbapenem compounds of formula (I) accord­ ing to the present invention are suitably administered in the form of parenteral formulations, particularly in the form of injectable solutions. The production of the carbapenem compounds of the formula (I) according to the present invention will be described more in detail by way of working examples. In the following description, the following symbols are used to have the particular meanings. ph : phenyl group PNB : p-nitrobenzyl group PNZ : p-nitrobenzyloxycarbonyl group Si : tertiary-butyldimethylsilyl group Ac : acetyl group Et : ethyl group Example 1: To 15 g of hydrazine monohydrate in a 50 ml flask was added dropwise 9.3 g of epichlorohydrin at 0°C over 1 hour. After addition, the reaction mixture was stirred for 2 hours at the above temperature. After removal of the excess hydrazine under reduced pressure, 300 ml of a saturated sodium bicarbonate solution and 200 ml of tetrahydrofuran were added to the residue. To this solution was added dropwise a solution of 43 g of p-nitro­ benzyloxycarbonyl chloride in 150 ml of tetrahydrofuran. The reaction mixture was stirred for 2 hours. After the reaction, 200 ml of ethyl acetate was added to the re­ action mixture. The organic layer was separated and the water layer was extracted with 100 ml of ethyl acetate. The organic layers were combined and washed with a saturated sodium chloride aqueous solution and dried over sodium sulfate. The solvent was removed and 500 ml of chloroform was added to the residue. The resulting solution was stored in a refrigerater to give a pre­ cipitate. After removal of the precipitate, the solvent was removed and the residue was purified by silica gel column chromatography (dichloromethane) to give 16.7 g of Compound (1). NMR (CDCl₃) δ : 8.15 (4H, d), 7.48 (4H, d) 5.4-5.0 (1H, m), 5.37 (4H, s), 4.4-3.2 (4H, m). Example 2: (a) To a solution of 16.6 g of Compound (1) ob­ tained by Example 1 and 5.6 g of triethylamine in 200 ml of dichloromethane was added dropwise a solution of 6.42 g of methanesulfonyl chloride in 20 ml of dichloromethane at 0°C, and the reaction mixture was stirred for 15 minutes at room temperature. After the reaction, the organic layer was washed with 200 ml of water, 200 ml of a saturated sodium bicarbonate solution and 200 ml of a saturated sodium chloride solution and then dried over sodium sulfate. The solvent was removed to give 16.6 g of a pale yellowish powder. (b) A solution of 9.6 g of the above powder, 3.13 g of potassium acetate and 250 ml of acetone was refluxed for 2 hours. After addition of 50 ml of water, the reaction solvent was removed and the resulting residue was extracted with ethyl acetate. The organic layer was washed with water, dried over sodium sulfate and removed. The resulting residue was purified using silica gel column chromatography by chloroform as an eluent to give 5.9 g of powder. (c) To a solution of 13.6 g of the above powder, 145 g of tetrahydrofuran and 145 ml of methanol was added 12.3 ml of 4% sodium methoxide-methanol solution at 0°C and the reaction mixture was stirred for 5 minutes. After the reaction, 1N-hydrogen chloride solution was added to the reaction mixture and the resulting acidic solution was extracted with ethyl acetate. After washing and drying, the solvent was removed to give 11.9 g (63%) of Compound (2) as pale brownish powder. NMR (CDCl₃) δ : 8.17 (4H, d), 7.48 (4H, d), 5.28 (4H, s), 4.5-3.2 (5H, m). Example 3: Tin triflate (3.712 g) was dissolved in 10 ml of anhydrous tetrahydrofuran under a nitrogen gas stream, and the resulting solution was cooled to 0°C. To this solution were added 1.3 ml of N-ethylpiperidine and a solution of 1.2 g of Compound (4) above in 7 ml of an­ hydrous tetrahydrofuran. The mixture was stirred for 2 hours at the above temperature. A solution of 1.42 g of Compound (3) in 2 ml of anhydrous tetrahydrofuran was added, and the resultant mixture was stirred for 1 hour. After the completion of the reaction, 100 ml of chloro­ form was added and the mixture was washed with a 10% cirtic acid aqueous solution. The organic layer sep­ arated was then dried over MgSO₄ and the solvent was removed. The residue was purified by silica gel column chromatography with a n-hexane:ethyl acetate (2-1:1) mixture to give 1.93 g (97%) of Compound (5) as a yellow solid. NMR (CDCl₃) δ : 0.07 (6H, s), 0.88 (9H, s), 1.21 (3H, d), 1.26 (3H, d), 3.30 (1H, dd), 3.38 (2H, t), 3.94 (1H, dd), 4.55 (2H, t), 6.24 (1H, bs). Tin triflate (57.0 g) was dissolved in 164 ml of anhydrous tetrahydrofuran under a nitrogen gas stream, and the resulting solution was cooled to 0°C. To this solution were added 19.9 ml of N-ethylpiperidine and a solution of 21.71 g of Compound (6) above in 123 ml of anhydrous tetrahydrofuran. The mixture was stirred for 1.5 hours at the above temperature. A solution of 1.42 g of Compound (3) in 123 ml of anhydrous tetrahydrofuran was added, and the resultant mixture was stirred for 1 hour. After the completion of the reaction, chloroform was added and the mixture was washed with a 10% citric acid aqueous solution and a sodium chloride aqueous solution. The organic solution separated was then dried over MgSO₄ and the solvent was removed. The residue was purified by silica gel column chromatography with n-hexane:ethyl acetate (2:1) to give 33.57 g (98%) of Compound (7) as a yellow solid, m.p. 85.5-86.5°C. NMR (CDCl₃) δ : 0.07 (6H, s), 0.90 (9H, s), 1.00 (3H, t), 1.23 (3H, d), 1.26 (3H, d), 2.90 (1H, dd), 3.50 (1H, dd), 6.10 (1H, bs). [α] 25 / D = +233.9° (c=0.77, CHCl₃). To a solution of 30.66 g of Compound (7) obtained in the step (B) above in 740 ml of anhydrous acetonitrile was added 12.13 g of imidazole, and the mixture was stirred under a nitrogen gas stream at room temperature for 5.5 hours. Then, 53.39 g of Mg(O₂CCH₂CO₂PNB)₂ was added, and the mixture was stirred overnight at 60°C. The resultant reaction mixture was concentrated under reduced pressure to 200 ml and 1 liter of ethyl acetate was added thereto. The organic layer separated was washed with a 1N-HCl aqueous solution, a 5% aqueous NaHCO₃ solution and an aqueous sodium chloride solution in this order. After drying over MgSO₄, the solvent was removed and the residue was purified by column chromatography with 800 g of silica gel to yield 34.47 g of Compound (8) as a colorless oil. NMR (CDCl₃) δ : 0.06 (6H, s), 0.87 (9H, s), 1.16 (3H, d), 1.20 (3H, d), 3.63 (2H, s), 5.27 (2H, s), 5.92 (1H, bs), 7.56, 8.24 (4H aromatic ring proton) Compound (8) obtained above was used in the following step (D) without further purification. To a solution of 37.47 g of Compound (8) obtained in the step (C) above in 392 ml of methanol was added 19.6 ml of concentrated HCl, and the mixture was stirred at room temperature for 1.5 hours. The reaction mixture was concentrated to approximately 100 ml, and 800 ml of ethyl acetate was added. After the mixture was washed with water and then wtih an aqueous sodium chloride slution, it was then dried over MgSO₄. The solvent was removed under reduced pressure to yield Compound (9) as a colorless oil. NMR (CDCl₃) δ : 1.25 (3H, d), 1.30 (3H, d), 2.90 (2H, m), 2.65 (2H, s), 3.83 (1H, m), 4.15 (1H, m), 5.27 (2H, s), 6.03 (1H, bs), 7.55, 8.27 (4H, aromatic ring proton) Compound (9) was then dissolved in 408 ml of anhydrous acetonitrile, and 36.31 g of dodecylbenzyl­ sulfonyl azide and 13.81 ml of triethylamine were added. After the mixture was stirred at room temperature for 20 minutes, the solvent was removed. The residue was purified by means of column chromatography with 800 g of silica gel using chloroform:acetone (2:1) to give 21.57 g [69.4% as total yields of Compounds (B), (C) and (D)] of Compound (10) as a colorless oil. IR (CHCl₃) cm⁻¹: 2150, 1750, 1720, 1650. NMR (CDCl₃) δ : 1.23 (3H, d), 1.30 (3H, d), 2.92 (1H, m), 3.50-4.30 (3H, m), 5.38 (2H, s), 6.40 (1H, bs), 7.57, 8.30 (4H, aromatic ring proton) [α] 21 / D = -41.6° (c=3.1, CH₂Cl₂). In 134 ml of ethyl acetate was dissolved 21.57 g of Compound (10) obtained in the step (D) above, and 0.065 g of rhodium octanoate was added. The solution was stirred at 80°C for 0.5 hour and the solvent was removed. The residue was dried to give Compound (11) as a solid. IR (CHCl₃) cm⁻¹: 2950, 2925, 1860, 1830. NMR (CDCl₃) δ : 1.22 (3H, d, J=8.0Hz), 1.37 (3H, d, J=6.0Hz), 2.40 (1H, bs), 2.83 (3H, q, J=8.0Hz), 3.28 (1H, d, d), 4.00-4.50 (2H, m), 4.75 (1H, s), 5.28 and 5.39 (2H, ABq, J=12Hz), 7.58, 8.24 (4H, aromatic ring proton) To a solution of 186 mg of Compound (11) obtained in the step (E) in 2 ml of anhydrous aceto­ nitrile were added 0.11 ml of diphenylphosphoric chloride and 0.09 ml of diisopropylethyl amine under cooling with ice, and the mixture was stirred for 0.5 hour at the same temperature. After the reaction mixture was concentrated, the residue was purified by silica gel column chromato­ graphy to yield 252 mg of Compound (12) as a white solid. NMR (CDCl₃) δ : 1.24 (3H, d), 1.34 (3H, d), 3.30 (1H, q), 3.52 (1H, m), 4.10-4.40 (2H, m), 5.20 and 5.35 (2H, q), 7.29 (1H, m), 7.58 and 8.18 (4H, d). Example 4: To a solution of 476 mg of Compound (12) ob­ tained in Example 3 in anhydrous acetonitrile was added a solution of 460 mg of Compound (2) obtained in Example 2 and 0.17 ml of diisopropylethyl amine, and the mixture was stirred for 40 minutes under a nitrogen atmosphere. Removal of the solvent left a residue that was in turn purified by means of silica gel column chromatography (chloroform:acetone = 3:1) to yield 667 mg (100%) of Compound (13). NMR (CDCl₃) δ : 1.24 (3H, d, J=6.0Hz), 1.35 (3H, d, J=6.0Hz), 3.2-4.9 (9H, m), 5.16 (1H, d, J=15.0Hz), 5.26 (2H, s), 5.47 (1H, d, J=15.0Hz), 7.3-7.7 (6H, m), 0.85-8.3 (6H, s). Example 5: To a solution of 667 mg of Compound (13) in 7 ml of tetrahydrofuran and 7 ml of water was added 120 mg of platinum oxide, and the catalytic hydrogenation was carried out at room temperature for 1 hour under a pres­ sure of 3.0 atmospheres. After removal of the catalyst, the solvent was removed to give 192 mg (74.0%) of Compound (14) after lyophilization. IR (KBr) cm⁻¹: 1750. NMR (D₂O-CD₃OD) δ: 1.23 (3H, d, J=6.0Hz), 1.40 (3H, d, J=7.0Hz), 3.3-4.4 (9H, m). Example 6: 192 mg of Compound (14) was dissolved in 15 ml of phosphate buffer solution (pH 7.0), and the pH of the solution was adjusted to 8.5 by 1N sodium hydroxide solution. To this solution was added 570 mg of ethyl formimidate hydrochloride, and the reaction mixture was stirred for 1 hour under ice-cooling. After the pH of the reaction mixture was adjusted to 7.0, the solvent was removed and the resulting residue was lyophilized. The resulting powder was purified using HP-40 column (water, 3% acetone-water) to give 76 mg (33.8%) of (1R,5S,6S)-­ 2-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]-triazolium-­ 6-yl)]thio-6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-­ 3-carboxylate [Compound (15)] after lyophilization. NMR (D₂O) δ: 1.32 (3H, d, J=6.0Hz), 1.40 (3H, d, J=6.0Hz), 3.3-4.4 (9H, m), 9.12 (2H, s). Example 7: Crystalline (1R,5S,6S)-2-[(6,7-dihydro-5H-­ pyrazolo[1,2-a][1,2,4]-triazolium-6-yl)[thio-6-[(R)-1-­ hydroxyethyl]-1-methyl-carbapenem-3-carboxylate [Cry­ stalline Compound (15)]: 45 mg of (1R,5S,6S)-2-[(6,7-dihydro-5H-­ pyrazolo[1,2-a][1,2,4]-triazolium-6-yl)]thio-6-[(R)-1-­ hydroxyethyl]-1-methyl-carbapenem-3-carboxylate was dissolved in 4 ml water. The resultant solution was filtered using a Membran® filter (0.22 µm). The filtrate was lyophilized to give amorphous (1R,5S,6S)-2-[(6,7-­ dihydro-5H-pyrazolo[1,2-a][1,2,4]-triazolium-6-yl)]­ thio-6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-3-­ carboxylate. This amorphous form of (1R,5S,6S)-2-[(6,7-­ dihydro-5H-pyrazolo[1,2-a][1,2,4]-triazolium-6-yl)]­ thio-6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-3-­ carboxylate was then again dissolved in 0.4 ml of water, and the resultant solution warmed to 40°C to completely dissolve the (1R,5S,6S)-2-[(6,7-dihydro-5H-pyrazolo-­ [1,2-a][1,2,4]-triazolium-6-yl)]thio-6-[(R)-1-hydroxy­ ethyl]-1-methyl-carbapenem-3-carboxylate. After storage under refrigeration conditions for a period of 3 hours, the sample was inspected and crystals were observed. The crystalline (1R,5S,6S)-2-[(6,7-dihydro-5H-pyrazolo­ [1,2-a][1,2,4]-triazolium-6-yl)]thio-6-[(R)-1-hydroxy­ ethyl]-1- methyl-carbapenem-3-carboxylate was washed with a small amount of 50% ethanolic solution in water, and the resultant crystals were dried at room temperature under a vacuum to yield 34 mg of crystalline (1R,5S,6S)-­ 2-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]-triazolium-6-­ yl)]thio-6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-3-­ carboxylate (75.6%). This crystalline product was in the form of colorless fine needle with a melting point of 215°C (decomp.), and showed characteristic peaks in planar spacings (d) 5.8, 5.5, 4.9, 4.8, 4.4, 4.3, 4.0, 3.5, 3.3, 3.2 and 3.0 in an X-ray diffraction pattern. The carbapenem compounds according to the present invention may be formulated in various pre­ paration forms. The above components were formulated into 100 ml of an injectable suspension. (2) Lyophilization: An appropriate amount of distilled water was added to 20 g of the sodium salt of Compound (15) to make a total volume of 100 ml. The above solution (2.5 ml) was filled in vials so as for each vial to contain 500 mg of the sodium salt of Compound (15) and lyophilized. The lyophilized vial was mixed in situ with approximately 3-4 ml of distilled water to make an injectable solution. (3) Powder: Compound (15) was filled in an amount of 250 ml in a vial and mixed in situ with about 3-4 ml of distilled water to make an injectable solution. The above components were mixed with each other and punched into tablets in conventional manner. Such tablets, as required, may be formulated into sugar coatings or film coatings in conventional manner. The component was mixed with each other and formulated into troches by punching in conventional manner. The component was mixed with each other and filled in conventional hard gelatin capsules. The above components were mixed with each other and formulated into dry syrups in conventional manner. Each of the components was mixed with each other and formulated in powders in conventional manner. The above components were mixed with each other and formulated into suppositories in conventional manner.

Claims: 1. A (1R,5S,6S)-2-substituted thio-6-[(R)-1-­ hydroxyethyl]-1-methyl-carbapenem-3-carboxylic acid derivative represented by the following formula wherein R¹ represents a radical of the formula and R² is a hydrogen atom or an anion charge, or a pharmacologically acceptable salt thereof. 2. The compound of claim 1 which is (1R,5S,6S)-­ 2-[4-pyrazolidinyl]-thio-6-[(R)-1-hydroxyethyl]-1-­ methyl-carbapenem-3-carboxylic acid of the following formula or a pharmacologically acceptable salt thereof. 3. The compound of claim 1 which is (1R,5S,6S)-2-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]-­ triazolium-6-yl)]thio-6-[(R)-1-hydroxyethyl]-1-methyl-­ carbapenem-3-carboxylate of the following formula or a pharmacologically acceptable salt. 4. The compound of claim 3 which is the compound of formula (I-2) in a crystalline form, or a pharma­ cologically acceptable salt. 5. The compound of any one of claims 1 to 4 as a pharmaceutically active substance. 6. (1R,5S,6S)-2-[(6,7-dihydro-5H-pyrazolo[1,2-a]­ [1,2,4]triazolium-6-yl)]thio-6-[(R)-1-hydroxyethyl]-1-­ methyl-carbapenem-3-carboxylate or a pharmacologically acceptable salt thereof as a pharmaceutically active substance. 7. The compound of any one of claims 1 to 4 as an antibacterial agent. 8. (1R,5S,6S)-2-[(6,7-dihydro-5H-pyrazolo[1,2-a]­ [1,2,4]triazolium-6-yl)]thio-5-[(R)-1-hydroxyethyl]-1-­ methyl-carbapenem-3-carboxylate or a pharmacologically acceptable salt thereof as an antibacterial agent. 9. A process for producing a (1R,5S,6S)-2-­ substituted thio-6-[(R)-1-hydroxyethyl]-1-methyl-­ carbapenem-3-carboxylic acid derivative represented by the following formula wherein R¹ represents a radical of the formula and R² is a hydrogen atom or an anion charge, or a pharmacologically acceptable salt thereof; which comprises reacting a compound represented by the follow­ ing formula wherein R³ is a carboxyl protecting group, and Ra is an acyl group, with a mercapto reagent represented by the following formula wherein Rb is an amino protecting group, to give a compound represented by the following formula wherein R³ and Rb have the same meanings as above, and subjecting the compound of the formula (IV) to re­ moval of the protecting groups R³ and Rb to give the carbapenem compound of the following formula and then, if desire, reacting the resulting compound of formula (I-1) with formimidic acid ester to give the carbapenem compound represented by formula (I-2) 10. A medicament containing a compound of formula (I) defined in claim 1 or a pharmacologically acceptable salt thereof. 11. A medicament comprising (1R,5S,6S)-2-[(6,7-­ dihydro-5H-pyrazolo[1,2-a][1,2,4]triazolium-6-yl)]thio-­ 6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-3-carboxylate or a pharmacologically acceptable salt thereof. 12. An antibacterial agent comprising a compound of formula (I) defined in claim 1 or a pharmacologically acceptable salt thereof. 13. An antibacterial agent comprising (1R,5S,6S)-­ 2-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazolium-6-­ yl)]thio-6-[(R)-1-hydroxyethyl]-1-methyl-carbapenem-3-­ carboxylate or a pharmacologically acceptable salt thereof. 14. The use of the compound according to any one of claims 1 to 4 for controlling or preventing a disease. 15. The use of the compound according to any one of claims 1 to 4 for controlling or preventing a bacterial infection. 16. The use of the compound according to any one of claims 1 to 4 for the manufacture of antibacterial medica­ ments. Claim for the Contracting States ES, GR A process for producing a (1R,5S,6S)-2-­ substituted thio-6-[(R)-1-hydroxyethyl]-1-methyl-­ carbapenem-3-carboxylic acid derivative represented by the following formula wherein R¹ represents a radical of the formula and R² is a hydrogen atom or an anion charge, or a pharmacologically acceptable salt thereof; which comprises reacting a compound represented by the follow­ ing formula wherein R³ is a carboxyl protecting group, and Ra is an acyl group, with a mercapto reagent represented by the following formula wherein Rb is an amino protecting group, to give a compound represented by the following formula wherein R³ and Rb have the same meanings as above, and subjecting the compound of the formula (IV) to re­ moval of the protecting groups R³ and Rb to give the carbapenem compound of the following formula and then, if desire, reacting the resulting compound of formula (I-1) with formimidic acid ester to give the carbapenem compound represented by formula (I-2)

Inventors: HIKIDA, MUNEO, KUMAGAI, TOSHI, MACHIDA, YOSHISUKE, MATSUNAGA, HIROSHI, NAGAO, YOSHIMITSU, NAGASE, YUNOSUKE
Applicants: LEDERLE (JAPAN) LTD.
Application year: 1988
Main classification: C07D487
IPC codes: A61K31, A61P31, C07D519, C07D477