METHOD OF TREATING VIRAL INFECTIONS IN HUMANS AND COMPOSITIONS THEREFOR

Abstract: A method of treating viral diseases in a human subject. The method involves applying effective amounts of the compound (I), wherein X is an azido group, a methoxy radical or a fluorine atom and B is thymine, uracil, guanine, cytosine, purine or hypoxanthine if X is methoxy or fluorine, and B is guanine, purine or hypoxanthine if X is azido or a pharmaceutically acceptable salt thereof. Also disclosed are compositions and compounds useful in the method.

Description: - ?- METH O D OF TREATING VIRAL INFECTIONS IN H UMANS AND COMPOSITIONS THEREFOR FIELD OF THE INVENTION This invention relates to a method of treating humans with diseases brought about by viruses which are characterized by RNA-dependent DNA polymerase. Of special interest are methods of treating retrovirus infections, especially those caused by Human Immunodeficiency Virus (HIV) , which is the cause of Acquired Immune Deficiency Syndrome (AIDS) . BACKGROUND AND PRIOR ART Patients exhibiting acquired immune deficiency syndrome (AIDS) were first observed in 1981. While occurrences were at first isolated and appeared to be confined to the male homosexual population, this observation very quickly proved to be wrong as AIDS was found to be widespread across all population groups. AIDS infection has been characterized as an epidemic in some parts of the world, and it is expected that other parts of the world will soon characterize the infection as such. In U.S. Patent No. 4,520,113, filed April 23, 1984, Gallo et al. stated that the evidence suggested that "the disease spreads by the transmission of an infectious agent. The primary targets of affliction in the human body are specific subpopulations of T-cells. The severe immune deficiency of these patients results from an unusually low proposition of helper T-cells (T4) in their lymphocyte population, thus reducing the availability of many T4 helper functions, among which is the production of antibodies by B-cells". Gallo, et. al., Science 220:865-867 (May 20, 1983) proposed that the causative agent of AIDS is a human T-cell leukemia virus (HTLV) . These viruses are characterized by pronounced tropism for T4 cells, reverse transcriptase with high molecular weight (100 kd) , and with preference for Mg~ for optimal enzymatic activity. Schtlpbach, et al. , Science 224:503-505 (May 4, 1984) . The work ot Barre-Sinussi, et al., Science 220:868-871 (May 20, 1983) , agreed with the Gallo hypothesis, and differed only in its calling the virus lymphadenopathy AIDS associated virus (LAV) . Currently, most scientists in the field refer to the virus as human immunodeficiency virus (HIV) , and this term will be used hereafter. Investigations began almost immediately for suitable methods and materials to treat HIV infections, which are always fatal. It must be pointed out that investigations proceeded on several levels. The first of these is on the "prevention" level, i.e., attempts to prevent infection from occurring. This approach involves modifying behavior patterns, and is not discussed further herein. The second approach has been investigations along the lines of developing an immune response to the virus, by way of a vaccine. Research has been slow in this area, for several reasons. HIV infections occur only in humans, and are always fatal. Vaccine research generally involves introducing an etiolated or dead strain of the virus to the subject in order to develop an immune response. Animal models do not work, and the risks involved in human trials have been too great to advance work in this field very far. Yet a third approach has been to treat the "follow-up" infections, such as Pseudomonas carinii pneumonia (PCP) , v/hich develop after HIV devastates the immune system. Sulfamethoxazole, sold as "Bactrim" has been one effective drug. It is extremely toxic, however, and many AIDs patience have allergies to it. These factors result in limited usefulness for the drug. Where sulfamethoxazole cannot be used, pentamidine isethionate, known as an anti-protozoan, is used. This drug has proven to be somewhat more effective when administered to AIDs patients in intravenous or aerosol form, but it too is extremely toxic and has various known and suspected side effects. Long term therapy with either of these substances is not possible. The fourth and final approach to treating HIV is via drugs which interfere with the virus' ability to replicate. This area has received much attention because, ultimately, it is the only approach which will destroy or limit the infection at its source. The current drug of choice in this area is azido- thymidine, also referred to as AZT. AZT, in severely limited trials, has been shown to interfere with viral replication, which results in some remission of the infection. There have, however, been problems with AZT as well. It is toxic, and is suspected of suppressing bone marrow function. See, e.g. Kolata, Science 225:1463 (March 20, 1987) . The regime which is used for treatment with AZT requires very stringent conditions which must be followed by the patient for the drug to be effective. In view of this, as well as AZT's toxicity, research did not and has not stopped following the positive results obtained with the drug. It is therefore an object to the invention to provide a method of treating humans infected with viruses characterized by RNA-dependent polymerases, especially those humans infected with human immuno-deficiency virus (HIV) . It is a further object of the invention to provide a composition useful in treating individuals infected with human immunodeficiency viruses. How these as well as other objects of the invention are achieved will be made explicit by the disclosure which follows. SUMMARY OF THE INVENTION A method of treating humans infected with viruses characterized by RNA dependent polymerases, comprising administering to an infected individual an effective amount of at least one compound of the formula wherein X is an azido group, a methoxy radical or a fluorine atom. B is thymine, uracil, guanine, cytosine, hypoxanthine, or purine if X is a methoxy radical or a fluorine atom and B is a guanine, purine or hypoxanthine radical if X is azido, as well as pharmaceutically acceptable salts thereof. Compositions are also prepared which comprise at least one compound as described supra, taken with conventional pharmaceutical carriers and diluents. BRIEF DESCRIPTION OF THE FIGURES Figure 1 shows graphically the pathogencity of HIV on MT 4 cells. Figure 2 shows results of in vitro viability studies when 3'-azido-2' ,3'-dideoxyguanosine was added to HIV infected cells. Figure 3 shows results of in vitro viability studies when 3'-fluoro-3'-deox thymidine was added to HIV infected cells. Figure 4 shows results of in vitro viability studies when 3'-fluoro-2' ,-3'-dideoxyguanosine was added to HIV infected cells. Figure 5 shows results of in vitro viability studies when 3'-azido-2' ,-3'-dideoxyinosine was added to HIV infected cells. DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS The active materials described supra will be seen to be 9-(beta-D-deoxyribofuranosyl) nucleosides of the thymidine uridine guanosine cytidine purinnucleoside or inosine series. Of particular interest are the specific compounds 3 ' -azido-2 ' , 3 '-dideoxy guanosine, 3 ' -azido-2 ' , 2 ' -dideoxy- inosine, 3 '-fluoro, 2 ' ,3 '-dideoxy guanosine and 3 ' -fluoro-3 '- deoxythymidine. The first compound is prepared according to the process described in Imazawa, et. al., J. Org. Chem. 4_3: 3044-3048 (1978) , the disclosure of which is incorporated by reference herein. It is surprising that the compounds used according to the invention are active against HIV because, from Clerco, et. al., Bioche . Pharm. 29: 1849-1851 (1980) , one is taught that 3 '-azido-2 * ,-3 ' -dideoxyguanosine shows no measurable antiviral activity against vaccinia virus, herpes simplex virus 1, or vesicular stomatitis virus. The preparation of 3 '-azido-2' ,-3 '-dideoxyguanosine takes place, in principle, by transglycosylation of the corresponding 5'-0-acetyl- or 5 '-O-trimethylsilyl-3 '-azido- 2 3 ' -deoxythymidme with silylated N -palmitoylguanine in the presence of trimethylsilyl-trifluoromethanesulphonate as catalyst. The corresponding hypoxanthine compound is obtained in an analogous manner by the use of the corresponding palmitoyl-hypoxanthine. Alternatively these guanine and hypoxanthine compounds may be obtained by the reaction of l-O-methyl-5-0- (4-methylbenzoyl) -3-azido-2 , 3-dideoxy-D- ribofuranose, obtained according to the procedure of N.D. Dyatkina and A.V. Azhayev described in Synthesis, 1984, p. 961, with silylated guanine or hypoxanthine with the help of a catalyst, for example trimethylsilyl-trifluoromethane- sulphonate, according to the procedure of H. Vorbruggen, K. Krokiewics and B. Benna, described in Chem. Ber. 114, p. 1234/1981. The corresponding guanine and hypoxanthine compounds with a fluorine atom or a methoxy residue in 3-position of the 2-deoxy-ribofuranosyl structure can be obtained from 3'-fluoro-3'-deoxy- or 3'-0-meth l-3-deoxy- thymidine by means of the same transglycosylation reaction. 2,3'-anhydro-1- ( -thymine (briefly called 0 2, 03'-eyelothymidine) , prepared according to G. Kowollik, R. GSrtner, P. Langen, Tetrahedron Letters, 1969, p. 3863, thereby again served as starting material. By reaction with sodium methylate the 3'-0-methyl-3'-deoxy- thymidine is prepared. 3'-fluoro-3'-deoxythymidine and its preparation is known from G. Etzold, R. Hintsche, G. Kowollik and P. Langen, Tetrahedron 21_ (1971) pp. 2463-2472. The preparation is achieved in principle by the reaction of cyclothy idine in diethylene glycol with potassium hydrogen difluoride. The reaction products are separated on a kieselgel column with a mixture of CHC1- and methanol. A second purification step was done by chromatography on a f LiChroprep RP-18 column with a mixture of 50 mM triethyl- ammoniumcarbonate/5 acetonitrile. 3 '-fluoro-3 ' -deoxythymidine can also be prepared by reacting the cyclothymidine, prepared by the method of G. Kowollik, G. Etzold, M. von Janta, Lipinski, K. Gartner, B. Langen in J. Prakt. Che . (1973) 315, p. 895, with hydrofluoric acid and aluminum trifluoride. Alternatively the cyclothymidine can be reacted with potassium fluoride in the presence the crown ether 18-crown-6 in dimethyl formamide or dioxane. Additionally the hypoxanthine compositions may be obtained from the corresponding adenine compositions, which are in part known from the literature cited above, by deamination with nitric acid or nitrosyl chloride. The synthesis of 3 '-fluoro-2 ' , 3 '-dideoxyuridine is carried out in analogy to the preparation of 3 '-fluoro-3 '- deoxythymidine (G. Etzold, R. Hintsche, G. Kowollik and P. Langen, Tetrahedron 2_7 (1971) pp. 2463-2472) . Thus the 2, 3-anhydro-l- (2 *-deoxy- p -D-xylofuranosyl-) -uracil (briefly called 0 2, 03 '-cyclo-deoxy-uridine) is synthesized in analogy to 0 2, 03 '-cycloethymidine (G. Kowollik, K. Gartner and P. Lange, Tetrahedreon Letters (1969) p. 3863) starting with 2 '-deoxyuridine. Cyclodeoxy-uridine is then reacted with potassium hydrogen difluoride in ethylene glycol to s70 yield the 3 ' -fluoro-2 ' ,3 '-deoxy-uridine. This composition served as starting material for the synthesis of 3 ' -fluoro-2' ,3 '-deoxycytidine by an amination reaction according to B.F.L. Li, C.B. Reese and P.F. Swann (Biochem. 26 (1987) pp. 1986-1093) or W.L. Sung (J. Org. Che . £7 (1982) pp. 3623-3628) by reaction with diphenyl phosphor- chloridate or triisopropylbenzenesulphonylchloride or in another suitable activating reagent in the presence of nitrotriazole, triazole, or another convenient nucleophile, followed by reaction with ammonia. The effectiveness of the active compounds used according to the present invention was ascertained in a biological system in which the HIV isolated from patients with AIDS and ARC was cultured with susceptable human T-cells. The in vitro pathogeneit of the so cultured HIV was determined with the use of MT 4.-cells, a T4.-cell line fo: which HIV is cytopathic. The MT -cell line is described in Science, 229 (1985) pp. 563-566. The cytopathological effect was thereby monitored by measurement of the incorporation of thymidine into the cells. If the active materials according to the present invention, especially 3 '-azido-2' ,3 ' -dideoxyguanosine was added to the MT.-cells inocculated with HIV, then, even with nano olar concentra¬ tions of the active materials it was possible to protect ? against the cytopathological effects brought about by the virus. The toxicity determination also took place in the same system. In this case, MT.-cells were exposed to the nucleoside analogue contained in the pharmaceutical composition according to the present invention as active materials in the absence of HIV and again the toxicity was determined on the basis of the incorporation of thymidine. From the so determined concentration for the achievement of an antiviral effect in comparison with the amount of active material necessary for a toxic effect, there was determined an in vitro therapeutic index. For the most preferred 3 '-azido-2',3 '-dideoxyguanosine compound, this therapeutic index, in the case of the human cell line used, was equal or greater than 10 , for 3 3 '-fluoro-3 '-deoxythymidine greater than 10 , for 3'-fluoro- 2 ' ,3 '-dideoxy-guanosine approximately 400. The experiments were carried out in such a manner, that samples of the supernatant of the cell line producing the virus were brought in stepwise dilution in microtiter plates which contained the MT.-cell line. At the same moment radioactive labelled thymidine was added to the microtiter plates and the incorporation of this substance, which runs parallel to the living activity of the cells, was measured. Under the ^ . influence of toxic concentrations of the active material according to the present invention or cytopathic amounts of virus-containing liquid, the incorporation of radioactively labelled thymidine decreased correspondingly. In Fig. 1 there is graphically illustrated the influence of different dilutions of the HIV virus-containing supernatant of the virus producing cell line (differing virus concentration) on the thymidine incorporation into MT,-cells. The incorporation curve shows that up to a dilution of 1:30 the pathogeneity of HIV viruses leads to a very low thymidine uptake increase correspondingly and does achieve the highest value at a dilution of the virus-containing supernatant of 1:10000. Thus, Fig. 1 shows, in the case of a dilution of the cultured supernatant of 1:40, an almost maximum pathogeneity of the HIV on the MT -cells is still present. The investigations of the effectiveness of the active materials to the pharmaceutical compositions according to the present invention took place in the case of this virus dilution in that a premixed solution of virus dilution and of the active material to be investigated was added to the cell line sample and the thymidine incorporation again determined. The results of these experiments with the preferred compound 3 ' -azido-2 ', 3 ' -dideoxyguanosine are shown in Fig. 2 of the accompanying drawing. Fig. 2 shows graphically the relationship between the amount of active material added in /Ug . /ml . and the rate of thymidine incorporation in cpm (counts per minute) . The curve indicated with -HIV was obtained in the absence of virus and the +HIV curve in the presence of virus. It shows that a concentraton of 0.3 /g./ml. of active material protects the cells with half maximum action against the pathogenic effect of a 1:40 dilution of the HIV parent liquid. Fig. 3 depicts the connection between the amount of 3 ' -fluoro-3 '-deoxythymidine added in //g./ml. and the thymidine incorporation in cpm (counts per minute) in analogy as explained for Fig. 2. It shows that at a concentration of less than 0,002 ^ /g./ml. the active compound protects the cells already with half maximal action against the pathogenic effect of a 1:40 dilution of the HIV parent liquid. In analogy to this Fig. 4 shows the in vitro antiviral activity of 3 '-fluoro-2 ',3 ' -dideoxyguanosine. The realiza¬ tion and interpretation was done according to the explanation for Fig. 2. This active material at a concen¬ tration of less than 1.5_ /g./ml. protects the cells already ^y with half maximal action against the pathogenic effect of the 1:40 dilution of HIV patent liquid. In Figure 5, experiments are presented in which 3'-azido-2' ,3'-dideoxyinosine was the drug administered. Toxicity of the inosine compound was higher, but again the rate of thymidine incorporation increased in the HIV infected cell line, and nearly equalled that of non-infected T4. cell lines. EXPERIMENT II In the following experiment, the suppression of HIV production in phytohemagglutinin (PHA) stimulated peripheral blood lymphocytes were studied. Samples of peripheral blood lymphocytes were stimulated with PHA, and this was followed by addition of HIV virus directly to the culture flasks in which the cells were grown. In those sample runs in which drug was added, this followed immediately after the introduction of the virus to the culture flask. The amount of HIV present following administration of the drug was determined by radioimmunoassay for the component of HIV known as "p24". The radioimmunoassay used has a sensitivity of 20-0.625 j cj/τ l tested. ^5- It was essential when running this test to determine the viability of the PBLS as well. This was done via incorporation of radioactive thymidine, as set forth in Example I, supra. Different concentrations of each drug were used: for azido-thymidine (AZT) , these were 1, 0.1, and 0.01 uM, while for 3 '-azido-2 ' ,3 '-dideoxy guanosine (ADG), the doses were 3, 0.3, 0.003, and 0.0003 ug. Positive controls ("+") , received HIV, but no drug, while negative controls ("-") , received drug, but no virus. Assays were run at 4, 7, 11, and 14 days of culture. At each point, the culture medium was replaced by fresh medium. These results are set out in Table 1: 76 Table 1 Pt/Sample Day Date Counts 1 Cc >unts 2 Av. Net %B/Bo ng p2 Total Counts 5639 5478 5423.5 Blank 151 138 144.5 0.0 "0"Standard 3187 2932 3059.5 2915.0 100.0% 0.625ng 2695 2724 2709.5 2565.0 88.0% 1.25ng 2492 2497 2494.5 2350.0 80.6% 2.5 g 1981 2105 2043 1898.5 65.1% 5. Ong 1494 1472 1483 1338.5 45.9% lO.Oπg 815 899 857 712.5 24.4% 20. Ong 546 498 522 377.5 13.0% AZT 1 4 1700 1684 1692 1547.5 53.1% 3. AZT 0 1 4 1600 1708 1689 1544.5 53.0% 3. 0.01 4 1759 1692 1725.5 1581.0 54.2% 3. 4 307 314 310.5 166.0 5.7% + 4 3209 3165 3187 3042.5 104.4% 4 2709 2771 2740 2595.5 89.0% ADG 3 4 1587 1730 1658.5 1514.0 51.9% 3. ADG 0, 3 4 1215 1208 1211.5 1067.0 36.6% 6. ADG 0, 03 4 357 360 358.5 214.0 7.3% ADG 0, 003 4 396 376 386 241.5 8.3% AZT 1 7 2310 1957 2133.5 1989.0 68.2% 2. AZT 0. 1 7 2304 2369 2336.5 2192.0 75.2% 1. AZT o.01 •7/ 1959 1960 1959.5 1815.0 62.3% 2. + 7 224 ' 232 228 83.5 2.9% + 7 293 239 266 121.5 4.2% 7 2649 2839 2744 2599.5 89.2% ADG 3 7 2255 2255 2110.5 72.4% 1 ADG 0 7 1047 843 945 800.5 27.5% ADG 0, 03 7 316 262 289 144.5 5.0% ADG 0 003 7 294 283 288.5 144.0 4.9% AZT 1 11 2458 2483 2470.5 2326.0 79.8% 1. AZT 0, 1 11 2531 2340 2435.5 2291.0 78.6% 1. AZT 0, 01 11 501 485 493 348.5 12.0% + 11 249 236 242.5 98.0 3.4% + 11 242 260 251 106.5 3.7% 11 2770 2817 2793.5 2649.0 90.9% ADG 11 2653 2592 2622.5 2478.0 85.0% 0. ADG 3 11 277 287 282 137.5 4.7% ADG 03 11 225 242 233.5 89.0 3.1% ADG 003 11 201 225 213 68.5 2.3% AZT 14 2652 2558 2605 2460.5 84.4% 0. AZT 1 14 2151 2048 2099.5 1955.0 67.1% 2. AZT 01 14 351 299 325 180.5 6.2% + 14 277 309 293 148.5 5.1% + 14 227 245 236 91.5 3.1% 14 2393 2665 2529 2384.5 81.8% 1 ADG 3 14 2725 2721 2723 2578.5 88.5% 0 ADG 0, 3 14 316 297 306.5 162.0 5.6% ADG 0, 03 14 292 282 287 142.5 4.9% ADG 0, 003 14 288 260 274 129.5 4.4% 4 ? It is interesting to note that ADG, at a concentration of 3 ug, was slightly more effective than AZT. Both AZT and ADG began to lose efficacy at their lowest concentrations at about the same time. The drugs do not show toxicity against the PBL either, as the "counts" resulting from thymidine indicate that cell viability remained good. Table 2 presents this data in term of a RIA adjusted for 10 6 cells/ml. Table 2 Pt/Sa ple Day NET Cnts CPM/ l CELL+06 CPM/1+06 CEL AZT 1UM 7 380 0 2 ,9E+03 3.72 7, .66E+02 AZT 0.1UM 7 47 0 3, 5E+02 4.08 8, .64E+01 AZT 0.01UM 7 784 0 5, 9E+03 3.3 1, .78E+03 + CONTROL 7 41 ,186 0 3, 1E+05 2.92 1, .06E+05 + CONTROL 7 62,298 0 4, 7E+05 3.68 1, .27E+05 - CONTROL 7 21, 0 1, 6E+02 4 3, .94E+01 ADG 3UG 7 325, 0 2.4E+03 3.7 6, .59E+02 ADG 0.3UG 7 1,704, 0 1.3E+04 3.92 3. .26E+03 ADG 0.03UG 7 36,160, 0 2.7E+05 2.68 1. ,01E+05 ADG 0.003UG 7 43 ,236. 0 3.2E+05 3.78 8. ,58E+04 AZT 1UM 11 (1,.084. 0) 8.1E+03 5.02 -1. , 62E+03 AZT 0.1UM 11 163. 0 1.2E+03 4.9 2. •49E+02 AZT 0.01UM 11 3,681. 0 2.8E+04 3 ,84 7. .19E+03 + CONTROL 11 44,567, 0 3.3E+05 4 ,22 7. .92E+04 + CONTROL 11 48,778. 0 3.7E+05 4 44 8. .24E+04 - CONTROL 11 (1,225.0) - 9.2E+03 5 ,72 -1. .61E+03 ADG 3UG 11 (1,280 0) 9.6E+03 5 ,84 -1. ,64Ξ+03 ADG 0.3UG 11 24,287 0 1.8E+05 4 ,84 3. .76E+04 ADG 0.03UG 11 52,529 0 3.9E+05 4 48 8. .79E+04 ADG 0.003UG 11 38,738 0 2.9E+05 1.7 6. .18E+04 AZT 1UM 14 76 0 5.7E+02 3 68 1. •55E+02 AZT 0.1UM 14 519 0 3.9E+03 3, 98 9. ,78E÷02 AZT 0.01UM 14 18 411 0 1.4E+05 5, 74 2. .41E+04 + CONTROL 14 15 ,864 0 1.2E+05 5, 02 2. .37E+04 + CONTROL 14 27,525.0 2.1E+05 4. 72 4. .37E+04 - CONTROL 14 (405, 0) 3.0E+03 3. 98 -7. .63E+02 ADG 3UG 14 (12, 0) 9. OE+01 3.5 -2. .57E+01 ADG 0.3UG 14 19 ,307, 0 1.4E+05 3.08 4. .70E+04 ADG 0.03UG 14 30,818.0 2.3E+05 3.66 6. •32E+04 ADG 0.003UG 14 31,978.0 2.5E+05 4.36 5. ,67E+04 These results show that ADG is effective in doses of from 0.3 to 3 ug/ml. One would expect similar results for 3 ' -azido-2'-3 ' -dideoxyinosine. EXAMPLE III . The Experiment as described in Example II was repeated for 3 ' -fluoro-2 '-3 '-dideoxyguanosine. 3 '-fluoro-2' -3 '- dideoxyguanosine was synthesized in analogy to the synthesis of 3 ' -azido-2' ,3 ' -dideoxyguanosine (Imazawa and Eckstein, J. Org. Chem. 43: 3044) (1978) by transgiycosidation of 3 ' -fluoro-3 '-deoxythymidine (synthesized according to G. Etzold et al. , Tetrahedron 27:2463) (1971) with palmitoyl guanine in acetonitrile under reflux for 60 minutes with bis (trimethylsilyl) -acetamide and trimethylsilyl trifluoromethanesulfonate. Chromatography on Kieselgei yields a product which on TLC (CHCl_/MeOH, 9:1) has a slightly larger R_ (0.43) than thymine (0.38) . It is reacted at 50°C overnight in MeOH saturated with NH.. After evaporation to dryness the residue is dissolved in H_0/MeOH and chromatographed on a LiChroprep RP-18 column with 50 mM triethy1ammonium bicarbonate (pH 7.5) containing 6% acetonitrile. After evaporation of the product-containing fractions and reevaporation with MeOH a crystalline residue of 9-(3-fluoro-2,3-dideoxy- -D-ribofuranosyl) guanine is obtained; mP>230°, X H-NMR (DMSO) , j , 6.15 pp (dd, 1H, J 3 R p = 53 Hz, H=3') , 4.14 (dm, 1H, J 4 H/3 . F = 32 Hz, H-4 ' ) HPLC on ODS-Hypersil with a linear gradient of 100 mM triethylammonium bicarbonate (pH 7.5) and acetonitrile (0 - 15% in 15 minutes) , retention times: 8.8 min for the -anomer, 6.8 min for the Oό-anomer. Yield Approx. 15%. The pharmaceutical compositions according to the presen invention can contain the active material in free form or in the form of physiologically acceptable salts, namely in the form of a compound or a mixture of compounds of the above given general formula. In addition, the pharmaceutical composition can contain pharmaceutical additives and diluent usual for the intended form of administration. Administration can be oral, parenteral, intrathecal, or intravenous, with oral administration being preferred. Preferred dosages range from about 1 to about 20g per day per 70 kilograms of body weight when administered to a human subject. Optimum dosage and mode of administration will vary from subject to subject. While there have been described what are at present considered to be the preferred embodiments of this invention, it will be obvious to one skilled in the art that various changes and modifications may be made therein without departing from the invention, and it is, therefore, aimed to cover all such changes and modifications as fall within the true spirit and scope of the invention.

Claims: 2^ WE CLAIM : Method for treating an infection of a virus characterized by RNA dependent polymerase in a human comprising administering to a human with an infection of a virus characterized by RNA dependent polymerase activity an anti-viral effective amount of a compound of formula wherein X is an azido group, a methoxy radical or a fluorine atom and B is thymine, uracil, guanine, cytosine, purine or hypoxanthine if X is methoxy or fluorine, and B is guanine, purine or hypoxanthine if X is azido, or a pharmaceutically acceptable salt thereof, 2. Method of claim 1, wherein X is fluorine or methoxy and B is thymine, guanine, cytosine, purine or hypoxanthine. 1 7 3. Method of claim 1, wherein said compound is designated 3 ' -azido-2 ' ,3' -dideoxyguanosine. 4. Method of claim 1, wherein said compound is designated 3 ' -fluoro-3 ' -deoxythymidine. 5. Method of claim 1, wherein said compound is designated 3 ' -fluoro-2 ' , 3 '-dideoxyguanosine. 6. Method of claim 1, wherein said compound is designated 3'-azido-2 ' , 3 '-dideoxyinosine. 7. Method of claim 1, wherein said virus is human immuno¬ deficiency virus. 8. Method of claim 1, wherein X is azido. 9. Method of claim 1, wherein X is methoxy. 10. Method of claim 1, wherein X is fluorine. 11. Method of claim 1 , wherein B is thymine. 12. Method of claim 1, wherein B is uracil. I L 13. Method of claim 1, wherein B is guanine. 14. Method of claim 1, wherein B is cytosine. 15. Method of claim 1, wherein B is purine. 16. Method of claim 1, wherein 3 is hypoxanthine. 17. Method of claim 1, wherein said compound is administere orally, parenterally, intrathecall , or intravenously. 18. Method of claim 1, wherein said compound is administere orally. 19. Method of claim 1, wherein said compound is administere in an amount from about Ig to about lOg/day per 70 kg o body weight. 20. Composition for the treatment of an infection characterized by an RNA-dependent DNA polymerase comprising an antiviral effective amount of a compound of the formula: ZJ wherein X is an azido group, a methoxy radical, or a fluorine atom and B is a thymine, uracil, guanine, cytosine, purine or hypoxanthine radical if X is methoxy or fluorine, and B is guanine, purine or hypoxanthine radical, if X is azido group or a pharmaceutical acceptable salt thereof, and a pharmaceutically accept¬ able carrier. 21. Composition of claim 20, wherein X is fluorine atom or methoxy radical and B is thymine, uracil, guanine, cytosine, purine or hypoxanthine. 22. Composition according to claim 20, wherein said compound is 3 '-azido-2 ' ,3 '-dideoxyguanosine. 23. Composition according to claim 20, wherein said compound is 3 ' -fluoro-3 '-deoxythymidine. 24. Composition of claim 20, wherein said compound is 3 ' -fluoro-2 ' ,3 '-dideoxyguanosine. 25. Composition of claim 20, wherein said compound is 3 ' -azido-2 ' , 3 '-dideoxyinosine. 26. Composition according to claim 20, wherein said compound is present in an amount from about 1 to about 20g per daily dosage. 27. 3 '-fluoro-2' ,3'-dideoxyguanosine. 28. 3'-azido-2' ,3'-dideoxyinosine.

Inventors: ECKSTEIN FRITZ, HARTMANN HEINZ, HUNSMANN GERHARD, ECKSTEIN, FRITZ, HARTMANN, HEINZ, HUNSMANN, GERHARD
Applicants: DEUTSCHES PRIMATENZENTRUM GMBH, MAX PLANCK GESELLSCHAFT, MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSC
Application year: 1987
Main classification: A61K31
IPC codes: A61K31, A61P31, C07H19