2,4,4-TRI- AND 2,2,4,4-TETRA SUBSTITUTED-1,3-DIOXOLANE COMPOUNDS

Abstract: 2,4,4-tri- and 2,2,4,4-tetra substituted-1,3-dioxolane compounds and pharmaceutically acceptable salts and solvates thereof which possess anti-fungal, anti-allergy, anti-inflammatory and/or immuno modulation activity. Methods for preparing and using the compounds also are described.

Description: -/- 2,4,4-TRI- AND 2,2,4,4-TETRA SUBSTITUTED- 1,3-DIOXO ANE COMPOUNDS This invention relates to 2,4,4-tri- and 2,2,4,4-tetra substituted-l,3-dioxolanes which exhibit antifungal, antiallergy, and/or immuno odulating activities, pharmaceutical compositions thereof, and , methods of their use in a host, including warm-blooded animals such as humans. This invention also relates to intermediates for preparation of the compounds. U.S. Patents Nos. 3,575,999, 3,936,470, 4,144,346 and 4,223,036 disclose 1,3-dioxolanes disubstituted at the 2-position by imidazol-1-ylmethyl or triazol-1-ylmethyl and phenyl or substituted phenyl and at the 4-position by hydrogen and substituted phenoxymethyl. Japanese Ko ai 83-128,383 (published June 30, 1983) discloses 1,3-dioxolanes substituted at the 4- position by triazol-l-ylmethyl and by chloro substituted phenyl. The triazole compounds are useful as germicides, plant growth controlling agents and herbicides in the field of agriculture and horticulture. None of the references disclose the compounds of the present invention. The present invention provides a compound represented by the formula I wherein Ar is thienyl, pyridyl, biphenyl, phenyl or phenyl substituted by one or more of halo, nitro, cyano, lower alkyl, lower alkoxy or perhalo lower alkyl; Y is CH or N; is -NR5-, -0- or -S(0) n ~; X is N0 2 , NRg γ or CORs; R^, R 2 , R3 and R4 are independently hydrogen or lower alkyl; R5 is hydrogen, lower alkyl or (C 2 -Cg)alkanoyl; Rs and R7 are independently hydrogen, lower alkyl, phenyl or phenyl substituted by one or more of halo, perhalo lower alkyl, (C 2 -Cg)alkanoyl, lower alkyl, lower alkoxy or 2-lower alk l-oxo-1,2,4-triazol-4- yl or Rg and R7 taken together with the nitrogen in NR5R7 form a substituted or unsubstituted five or six membered heterocyclyl ring containing one to four hetero atoms chosen from N, 0 and S(0) n / said heterocyclyl substituents being (C 2 -c 8 )alkanoyl, lower alkyl, phenyl or phenyl substituted by one or more of halo, perhaloloweralkyl, (C 2 -Cg)alkanoyl, lower alkyl, lower alkoxy, or 2-loweralkyl-3-oxo-l,2,4-triazol-4-yl; Rg is lower alkyl, lower alkoxy, - R-^R 2 , phenyl or phenyl substituted by one or more of halo, perhalo lower alkyl, lower alkoxy, nitro, cyano, (C -Cg)alkanoyl; p is 0, 1, 2, 3, 4 or 5; n is 0, 1 or 2; and the sterochemical isomers thereof in racemic or optically active form; or a pharmaceutically acceptable salt thereof. The present invention also is directed at an intermediate compound represented by formulae II or XX: (XX )+TRANS-(XX) wherein Ar Y, R^ and R are as previously defined for formula I, LG is a leaving group; and the stereochemical isomers thereof in racemic or optically active form. The compounds represented by formula II are intermediates for the preparation of the compounds represented by formula I. The compounds of formula I may be formulated into pharmaceutical compositions with a pharmaceutically acceptable carrier or diluent. The present invention also is directed at the use of a compound of formula I for the preparation of a pharmaceutical composition for hyperproliferative skin disease, anti-inflammatory, anti-fungal, arbti-allergy and/or immunomodulating applications. The present invention in addition is directed at a compound of formula I in combination with a pharmaceutically acceptable carrier. The present invention also comprises mixing a compound of formula, I with a pharmaceutically acceptable carrier. The. present invention also comprises a process for producing a compound of structural formula I in claim 1 characterized by: (a) reacting a compound of the formula with an anion <P in an aprotic solvent; (b) reacting a compound of the formula a compound of the formula (RO) where R is an alkyl; and (c) reacting a compound of the formula with a compound of the formula M-LG where M is The present invention also is directed at a method for treating and/or preventing fungal infections. hyperproliferative skin disease, inflammation, allergic reactions, autoimmune reactions and diseases and/or immunological diseases, comprising administering an effective amount of a compound of formula I. Preferred compounds of the present invention comprise: (■b)cis-l-acetyl-4-[4-[ [4-(4-chlorophenyl)-4- (1H-1,2 r 4-triazol-l-ylmethyl)-1,3-dioxolan-2-yllmethoxy] phenyl] iperazine; (±)trans-l-acetyl-4-[4-[ [4-(4-chlorophenyl)-4- (1H-1,2,4-triazol-l-ylmethyl)-1,3-dioxolan-2-yl]methoxy] phenyl] iperazine; (±)cis-4-[4-[4-[ -[ [ -(2,4-difluorophenyl)-4- (1H-1,2,4-triazol-l-ylmethyl)-1,3-dioxolan-2yl]methoxy] phenyl]1-piperazinyl]phenyl]-2,4-dihydro-2-(1- methylpropyl)-3H-l,-2,4-t iazole-3-one; (±)_cis_-l-[4-[[4-(2,4-difluorophenyl)-4-(1H- 1,2,4-triazol-l-ylmethyl)-1,3-dioxolan-2-yl]methoxy] phenyl]-4-(1-meth leth l) piperazine; methyl(±)cis-6-[ [ [4-(2,4-difluorophenyl)-4-(1H- 1,2,4-t iazol-l-ylmethyl)-1,3-dioxolan-2-yl]methyl] thio]hexanoate; (±)cis-6-[ [[4-(2,4-difluorophenyl)-4-(1H-1,2,4- t iazol-l-ylmethyl)-l,3-dioxolan-2-yl]methyl] thio] hexanoic acid; ( ±)cis-l-acetyl-4-[4-[ [4-(2,4-dichlorophenyl)- 4-(lH-imidazol-l-ylmethyl)-l,3-dioxolan-2-yl]methoxy] phenyl] iperazine; (±)cis-l-acetyl-4-[4-[ [4-(2,4-difluorophenyl)- 4-(1H-1,2,4-triazol-l-ylmethyl)-1,3-dioxolan-2-yl] methoxy]phenyl]piperazine; (±) trans-l-acetyl-4[4-[ [4-(2,4-dichlorophenyl)- 4-(lH-imidazol-1-ylmethyl)-1,3-dioxolan-2-yl]methoxy] phenyl] iperazine; and (±)cis-l-acetyl-4-[4-[ [4- (2,4-dichloroρhenyl)- 4-(lH-imidazol-1-ylmethyl)-2-methyl-l,3-dioxolan-2- yl]methoxy] henyl] iperazine. As used herein the term "halogen" means bromine, chlorine or fluorine, with chlorine and fluorine being preferred; fluorine is most preferred. The term "lower alkyl" refers to straight and branched chain hydrocarbon groups of 1 to 6 carbon atoms, such as methyl, ethyl, n-, and iso-propyl, n-, sec- and tert-butyl, n-, sec-, iso-, tert- and neo-pentyl, n-, sec-, iso- and tert-hexyl. The term "perhaloloweralkyl" refers to "lower alkyl" groups having only halogen substituents, e.g., -CCl-^-CF-^, -CP 2 -Cl3 as well as perhalo groups such as -CF 2 -CF 3 or -CF 3 ; trifluoromethyl is preferred. The term " (C 2 ~Cg)alkanoyl" refers to .straight and branched chain alkanoyl groups having 2 to 8 carbon atoms such as acetyl, propanoyl, butanoyl, 2- methylpropanoyl, 3-methylpropanoyl, pentanoyl, 2- methylbutanoyl, 3-methylbutanoyl, 4-methylbutanoyl, hexanoyl, 2-methylpentanoyl, 3-methylpentanoyl, 4- methylpentanoyl, 5-methylpentanoyl, heptanoyl, 3- methylheptanoyl, octanoyl, 2-ethylhexanoyl and the like. Acetyl is preferred. The term "lower alkoxy" means a lower alkyl moiety univalently bonded to divalent oxygen, -0- and includes methoxy, ethoxy, n- and iso-propoxy, n-, sec- and tert-butoxy. The term "2-lower alkyl-3-oxo-l,2,4-triazol-4- yl" means a moiety represented by the formula LOWER ALKYL wherein "lower alkyl" is as defined hereinabove. The term "heterocyclyl" refers to five and six- membered ring systems containing at least one carbon and one to four heteroatoms chosen from N, 0 and S, SO and S0 . Typical suitable heterocyclyls include morpholino, thioπtorpholino, 4-oxothiomerpholino, 4,4-dioxothio- morpholino, piperazino, pyrrolidino, piperidino, i idazolyl, 1,2,4-triazolyl, furanyl, thienyl, thiadiazolyls, especially l,2,3-thiadiazol-4-yl, and l,2,3-thiadiazol-5-yl, thiomorpholino, and pyridyls. The heterocyclyl may be attached via a carbon atom, e.g., N- methylpiperidin-4-yl, N-methylmorpholin-2-yl or via the nitrogen atom, e.g., piperidin-1-yl (commonly called piperidino) , morpholin-4-yl (commonly called morpholino) ,N-rmethylpiρerazin-4-yl (commonly called N- methylpiperazino) , lIT-1-imidazol-l-yl or 4H-1,2,4- triazol-4-yl. IH-l-imidazol-l-yl, 4H-1,2,4-triazol-4-yl and piperazino are the preferred heterocyclyls. Substituted heterocyclyls include lower alkyl heterocyclyls especially N-loweralkylheterocyclyls such as N-methylmorpholin-4-yl, N-ethylpiperazino, N-(l- methylethyl)piperazino, but also 2-methylpyrrolidino, 4- methylpipe idino, 5-methyl-lH-l,2,4-triazol-3-yl, 3- methyl-l-phenyl-lH-l,2,4-triazol-5-yl, and 2- methylpyridyl; (C 2 ~C3)alkanoyl heterocyclyls such as 2- acetylthiophenyl, 2-acetylpyrrolidino; haloheterocyclyls such as 2-halo-3-thienyl, 2,5-dihalo-3-thienyl, and 5- halo-2-thienyl; N-(C 2 -Cg)alkanoyl heterocyclyls such as N-acetylpiperazino and 4-acetylpiperidino; and ary substituted heterocyclyls include heterocyclyls substituted by phenyl or substituted phenyl as defined herein such as N-phenylpiperazino, N-(4- chlorophenyl)piperazino, 2-(4-trifluoromethyl- phenyl)piperazino, and N-(p-toluyl)piperazino, N-(4- methoxyphenyl)piperazino. Piperazino is the preferred substituted heterocyclyl. As used herein, the term "leaving group" (LG) means leaving groups readily removable under conventional conditions well known to those skilled in the organic synthetic arts so as to form the compound represented by formula I. Typical suitable leaving groups include halides especially bromide but also iodide,- trifluoro- methylsulfonyloxy, and 4-methylphenylsulfonylox . Compounds of the present invention represented by formula I can exist in two isomeric forms, cis and trans. With reference to formulas I and II "cis" means Ar and R- j _ are on the same side of the plane defined by the 1,3-dioxolane moiety. For example, l-[4-[ [4-(2,4- difluorophenyl)-4-(1H-1,2,4-triazol-l-ylmethyl)-1,3- dioxolan-2-yl]methoxy]phenyl]-4-(1-methylethyl)piperazine exists in the cis-2,4 and trans-2,4 forms as indicated by the formulas hereinbelow: In the formula labelled "cis-2,4" the hydrogen and 2,4-difluorophenyl groups are both positioned on the same side or face or plane (below) of the formula. In the "trans-2,4" formula, the groups are positioned on opposite faces of the formula. Both forms are considered within the scope of this invention as are individual optical isomers e.g., (+)-cis-2,4 and (-)-cis-2,4, each of which can be obtained by resolution of a racemic mixture [ (±)-cis-2,4] by conventional means well known to those skilled in the art. Compounds of formula II may also be resolvable, i.e. they exist as individual optical isomers or mixtures thereof. The compounds represented by formula I exhibit broad spectrum antifungal activity in conventional antifungal screening tests against human and animal pathogens, such as the following: Aspergillus, Candida, Epidemophyton, Geotrichum, Monosporiurn, Rhodotorula, Saccharomyces, Torulopsis and T ichophyton. The compounds of formula I exhibit topical and oral fungal activity in in vivo tests in animals that is comparable to that for ketoconazole, a commercial product. The preferred pharmaceutically acceptable salts are nontoxic acid addition salts formed by adding to the compounds of the present invention about a stoichiometric amount of a mineral acid, such as HCl, HBr, H SO * or H2PO , or of an organic acid, such as acetic, propionic, valeric, oleic, palmitic, steariσ, lauric, benzoic, lactic, para-toluene sulfonic, methane sulfonic, citric, maleiσ, fumaric, succinic and the like. The pharmaceutical compositions of the present invention may be adapted for oral, parenteral, topical or vaginal administration. They are formulated by combining the compound of formula I or an equivalent amount of a pharmaceutically acceptable salt thereof with any suitable, inert, pharmaceutically acceptable carrier or diluent. Examples of suitable compositions include solid or liquid compositions for oral administration such as tablets, capsules, pills, powders, cachets, granules, solutions, suppositories, " suspensions or emulsions. The powders and tablets preferably contain from 5 or 10 to about 70 percent of the active ingredient. Suitable solid carriers may be, for example magnesium carbonate, and magnesium stearate. Tablets, powders, cachets and capsules can be used as solid dosage forms suitable for oral administration. They may also be manufactured in the form of sterile solid compositions which can be dissolved in sterile water, physiological saline or some other sterile injectable medium immediately before use. Topical dosage forms may be prepared according to procedures well known in the art, and may contain a variety of ingredients, excipients and additives. The formulations for topical use include ointments, creams, lotions, powders, aerosols, pessaries and sprays. Of these, ointments, lotions and creams may contain water, oils, fats, waxes, polyesters, alcohols, or polyols, plus such other ingredients as fragrances, emulsifiers and preservatives. For preparing suppositories, a low melting wax such as a mixture of fatty acid glycerides or cocoa butter is first melted, and the active ingredient is dispersed homogeneously therein as by stirring. Liquid form preparations include solutions, suspensions and emulsions. As an example may be mentioned water or water-propylene glycol solutions for parenteral injection. Also included are solid form preparations which are intended to be converted, shortly before use, to liquid form"preparations for either oral or parenteral administration. Such liquid forms include solutions, suspensions and emulsions. Aerosol or non-aerosol sprays may be prepared usj.ng solutions or suspensions in appropriate solvents, e.g., difluorodichloromethane, for aerosols. Parenteral forms to be injected intravenously, intramuscularly, or subcutaneously are usually in the form of a sterile solution, and may contain salts or glucose to make the solution isotonic. A. Antifungal The topical dosage for humans for antifungal use in the form of a pharmaceutical formulation comprising a compound of formula I (usually in the concentration in the range from about 0.5% to about 20% preferably from about 1% to about 10% by weight) together with a non-toxic, pharmaceutically acceptable topical carrier, is applied several times daily to the affected skin until the condition has improved. In general, the oral dosage for humans for antifungal use ranges from about 1 mg per kilogram of body weight to about 50 mg per kilogram of body weight per day, in single or divided doses, with about 2 mg per kilogram of body weight to about 20 mg per kilogram of body weight per day being preferred. In general, the parenteral dosage for humans for antifungal use ranges from about 0.5 mg per kilogram of body weight per day to about 20 mg per kilogram of body weight per day, in single or divided doses, with about 10 mg per kilogram of body weight per day being preferred. B. Anti-allergy and Anti-inflammatory The compounds of this invention also are useful in treating or preventing an allergic reaction and/or inflammation in a host e.g. a warm blooded mammal such as man. The pharmaceutical compositions useful for treating or preventing allergic reactions and/or inflammation are analogous t to those described hereinabove in reference to the antifungal pharmaceutical composition. The compounds of formula I are effective non- adrenergic, non-anticholinergic, anti-anaphylactic agents. The compounds may be administered by any convenient mode of administration for treatment of allergic reactions employing an antiallergically, or antiinflammatory effective amount of a compound of formula I for such mode. In general, the oral dosage for humans for antiallergy and/or antiinflammatory use ranges from about 10 mg to about 500 mg per kilogram of body weight per day, in single or divided doses. Preferably the total dosages are administered in 2-4 divided doses per day. In general, the parenteral e.g. intravenous dosage for humans for antiallergy and/or antiinflammatory use ranges from about 0.1 mg per day to about 10 mg per kilogram of body weight per day, in single or divided doses. The compounds of formula I may be administered by inhalation (aerosol or nebulizer) . In general, the inhalatio.n dosage for humans for antiallergy use ranges from about 0.1 to 5 mg per puff, one to four puffs may be taken every 4 hours. C. Treatment of Hyperproliferative Skin Disease When administered for the treatment of hyperproliferative skin disease, e.g. psoriasis, a compound of formula I may be administered topically, orally, rectally or parenterally. When administered topically,, the amount of compound administered varies widely with the amount of skin being treated, as well as with the concentration of active ingredient applied to the affected area. When administered orally, the compounds of formula I are effective for the treatment of hyperproliferative skin disease at daily doses ranging from about 0.1 mg/kg of body weight to about 500 g/kg of body weight, preferably in 10 mg to 100 mg/kg of body weight, which may be administered in single or divided doses. When administered rectally, the compounds of formula I may be administered in doses ranging from about 0.1 mg to about 1000 mg. When administered parenterally, the compounds of formula I are effective for the treatment of hyperproliferative skin disease in daily doses ranging from about 0.1 mg/kg body weight to about 10 mg/kg body weight which may be administered in single or divided doses. Included within the invention are preparations for topical application to the skin whereby the compounds having structural formula I are effective in the treatment and control of skin diseases characterized by rapid rates of cell proliferation and/or abnormal cell proliferation, e.g., psoriasis. In a preferred method of treating hyperpro¬ liferative skin diseases, a pharmaceutical formulation comprising a compound of formula I, (usually in concentrations in the range of from about 0.01 percent to about 10 percent, preferably from about 1 percent to about 5 percent) together with a non-toxic, pharma¬ ceutically acceptable topical carrier, is applied several times daily to the affected skin until the condition has improved. Topical applications may then be continued at less frequent intervals (e.g. once a day) to control mitosis in order to prevent return of severe disease conditions. D. Immunomodulation Compounds represented by formula I also exhibit in vitro immunomodulating activity in a conventional screening test detailed hereinbelow. The compounds of formula I are useful as immunosuppressives as indicated by their inhibition of the proliferation of T cells and B cells and are therefore useful in treating autoimmune disease and reaction and other immunological diseases including bone marrow rejection, organ transplant rejection as well as skin graft rejection phenomena in a host, e.g. a warm blooded mammal such as man. The pharmaceutical compositions useful for treating autoimmune diseases and reactions in man are analogous to those described hereinabove in reference to the antifungal pharmaceutical composition for compounds of formula I. The compounds of formula I may be administered by any convenient mode of administration for treatment of autoimmune diseases, reactions and other immunological diseases by employing an immunomodulating effective amount of a compound of formula I for such mode. The topical dosage for humans for immuno¬ modulating use in the form of a pharmaceutaical formulation comprising a comopund of formula I (usually in the concentration in the range from about 0.1% to about 5% preferably from about 1% to about 3% by weight) together with a non-toxic, pharmaceutically acceptable topical carrier, is applied several times daily to the affected skin until the condition has improved. In general, the oral dosage for humans for immunomodulating use ranges from about 1 mg per kilogram of body weight to about 300 mg per kilogram of body weight per day, in single or divided doses, with about 50 mg per kilogram of body weight per day being preferred. In general, the parenteral dosage for humans for immunomodulating use ranges for about 25 mg per kilogram of body weight per day to about 300 mg per kilogram of body weight per day, in single or divided doses, with about 50 to about 100 mg per kilogram of body weight per day being preferred. It will be appreciated that the actual preferred dosages of the compounds of the present invention of formula I or pharmaceutically acceptable salts thereof will vary according to the particular composition formulated, the mode of application and the particular situs, host, the allergic reaction or disease being treated. Many factors that modify the action of the drug should be taken into account by the attending clinician, e.g., age, body weight, sex, diet, time of administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease or symptoms of the allergic and/or inflammatory reaction. The compounds are non-toxic when administered within the indicated dosage ranges. Administration can be carried out continuously or periodically within the maximum tolerable dose. Optimal application rates for a given set of conditions can be readily ascertained by the attending clinician using conventional dosage determination tests. As a result of the administration of a compound of formula I, a remission of the symptoms of the psoriatic patient, in most cases, can be expected. Thus, for example, one affected by psoriasis can expect a decrease in scaling, erythema, size of the plaques, pruritus and other symptoms associated with psoriasis. The dosage of medicament and the length of time required for successfully treating each individual psoriatic patient may vary, but those skilled in the art of medicine will be able to recognize these variations and adjust the course of therapy accordingly. The anti-allergy property of the compounds of formula I is evaluated by measuring the inhibition of the release of the mediator SRS-A (slow reacting substance of anaphylaxis) from sensitized guinea pig " lung fragments after antigen challenge. The test procedure utilized is described hereinbelow. Measurement of SRS-A Release From Sensitized Guinea Pig Lungs (a) Sensitization of Animals The release of SRS-A and histamine was studied in lungs from actively sensitized guinea pigs ' . Male Harley guinea pigs (250-300 g, obtained from Charles River or Dutchland Farms) were sensitized with 5 mg ovalbumin injected intraperitoneally and 5 mg subcutaneously in 1 ml saline on day one, and 5 mg ovalbumin injected intraperitoneally on day four. The sensitized animals were used 3-4 weeks later. (b) Release of SRS-A Sensitized guinea pigs were killed by a blow to the head and the lungs removed and cleaned of visable connective tissue, trachea and large blood vessels. The lungs from individual animals were sliced into fragments approximately 1 mm in thickness using a Mcllwain chopper and then washed with oxygenated Tyrode's buffer. Weighed aliquots (approximately 400 mg wet weight) of lung were transferred into vials containing 2 ml of fresh Tyrode's solution and incubated in the presence or absence of test compound 12 in at 37°C followed by challenge of the tissue with 20 μg ovalbumin/ml (final concentration). After an additional 15 min incubation, the vials were cooled to 4°C and 1.5 ml of clear supernatant media was removed and mixed with 6 ml of cold 100% ethanol. This mixture was thoroughly vortexed and kept at -15°C for 30 min to allow precipitation of.protein. The- samples were then centrifuged at 1000 x g for 15 min at 2°C -and the clear supernatant fluid removed into polyethylene tubes and taken to dryness at 50°C under a stream of gas. The samples were stored at -70°C until assayed for SRS-A by bioassay or radioimmune assay. The compounds of formula I inhibit the release of SRS-A from sensitized guinea pig lung fragments as measured using the test techniques described above. The compounds of formula I also inhibit 5- lipoxygenase and cyclooxygenase activity, which inhibitory activity has been associated with anti-allergy and anti-inflammatory activity. The compounds of formula I are thus useful for the treatment of allergies, allergic chronic obstructive lung diseases, inflammation, arthritis, bursitis, tendonitis, gout and other inflammatory conditions. The 5-lipoxyge ase inhibitory activity of the compounds of formula I may be demonstrated by the procedure described below: 5-Lipoxygenase and Cyclooxyqenase Assays with MC-9 Mast Cells The IL-3-dependent murine mast cell clone, MC- 9, was used to test the effects of compounds of formula I on cyclooxygenase and lipoxygenase activities. The MC-9 cell line was grown in suspension culture (0.4 to 1.2 x 10 6 cells/ml) in RPMI 1640 medium (Gibco) with 10% fetal bovine serum (Hyclone) and 2-5% conconavalin-A conditioned supernatant Musch et. al., Prostaglandins, pp 405-430,] (1985). Cells were harvested, washed twice by centrifugation, and resuspended in a Ca ++ -free HEPES buffer (25mM HEPES, 125mM NaCl, 2.5mM KC1, 0.7mM MgCl 2 , 0.5mM EGTA and 10 mM glucose at pH 7.4). MC-9 cells (0.39 ml at 7.5 x 10 6 cells/ml) were preincubated with DMSO vehicle with or without test compound (1 μl) for 4 min then incubated 5 -min with t C] arachidonic acid (Amersham, 59 Ci/mole) at a 9 μM final concentration and A23187 (Calbiochem) at a 1 μM final concentration added in 10 μl of water:ethanol (9:1). The reaction was stopped by addition of methanol (0.4 ml), and cellular debris was removed by centrifugation. Aliquots (250 μl) of the incubations were run on a Waters two pump HPLC system fitted with a Waters C18, 10 μ 8 x 100 mm μ-Bondapak radial compression column and C18 "Guard Pak". The column was initially eluted at 3 ml/min with water:methanol:acetic acid (67:33:0.08) containing ImM EDTA adjusted to pH 6.0 with ammonium hydroxide (Pump A). At 4 min, a linear gradient to reach 100% methanol (Pump B) at 9 min was established. Between 13 and 14 min, methanol was exchanged for the initial eluting solvent and by 19 min the column had been reequilibrated for the next sample. The effluent was analyzed by a continuous flow radioactivity monitor (model ROMONA-D) interfaced with a Hewlett Packard Lab Automation System for quantitation of radioactive products. -These included prostaglandin D which eluted at 4 min (PGD 2 ) and 5- hydroxyeicosatetraenoic acid (5-HETE) which eluted at 11 min (Musch, et al. (1985) Prostaqlandins 29, 405-430). The results with and without test compounds were used to calculate percent inhibition of PGD 2 and HETE production for compounds of formula I. These results are presented In Table 1. Table 1 Cone. % Inhibition of Compound (X1Q- 6 ml) PGP 5 HETE 15 34 100 \ Reference* 5 34 100 J 1 15 35 50 0 99 R* = COCH. 50 0 40 Reference* 50 -24 79 R ' = CH 2 ( H 3 ). 2 5 0 5 *NORDIHYDROGUAIARETIC ACID The compounds of formula I also are useful in the treatment of hyperproliferative skin disease, e.g., psoriasis, in mammals, e.g., humans, which may be demonstrated by their 5-lipoxygenase inhibitory activity as discussed above or by the Arachidonic Acid Mouse Ear Test as described below. Arachidonic Acid Mouse Ear Test, Materials and Methods Charles River, female, CD, (SD) BR mice, 6 weeks old, are caged 8 animals/group and allowed to acclimate 1-3 weeks prior to use. Arachidonic acid (AA) is dissolved in reagent grade acetone (2 mg/.Ol ml) and stored at -20°C for a maximum of 1 week prior to use. Inflammatory reactions are induced by applying 10 ml of " AA to both surfaces of one ear (4 gm total) . Test drugs are dissolved in either reagent grade acetone or aqueous ethanol (only if insoluble in acetone) at the same doses selected, by Opas et al. , Fed. Proc. 43, Abstract 2983, p. 1927 (1984) and Young et al. , J. Invest. Dermatol. 82, pp. 367-371 (1984) . These doses are employed to ensure maximum responses and to overcome any difference in topical absorption which could occur with any drug applied in an aqueous ethanol vehicle. The test drug is applied 30 minutes prior to challenge with AA. The severity of the inflammation is measured as a function of increased ear weight. A 6 mm punch biopsy is removed 1 hour after AA challenge and weighed to the nearest 0.1 mg. Mean ± standard error and all possible comparisons are made via Duncan's Multiple Range Statistic. The immunomodulating activity of compounds of formula I may be demonstrated by their inhibition of T cell and B cell proliferation in the following test. T- and B-Cell Mitogenic Responsiveness Spleen cells were obtained from six to eight week old C57 B1/6J male mice. One million viable spleen cells were cultured in triplicate in microtest II plates in the presence of 1 μg concanavalin A (Con A) 0.25 μg phytohemagglutinin (PHA) or 1.5 μg lipopolysaccharide (LPS) for 72 hours at 37°C. The total volume was 0.2 ml. One m crocuπe -Η-thymidine (specific activity, 2.0 Ci/mmole) was added for the last 16 hours of incubation. The cells were harvested and processed on a mash II harvester. A stock solution of 1x10 ώ of the drug was prepared in distilled water and then diluted with medium to the appropriate concentration. Drugs were added at the initiation of culture at concentrations of 0.1 to 100 μg. Immunomodulatory activity was determined by measuring percent inhibition of T cell proliferation for Con A and PHA and of B cell proliferation or LPS. Compounds exhibiting >60% inhibition were determined to have great immunomodulating activity. These results are presented in Table 2 for the compound having the following structure Table 2 Percent Inhibition Concentration T Cell Proliferation B Cell Proliferation (W ) Con A PHA LPS 0.1 0 0 4 1 100 99 63 100 100 100 100 The compounds of this invention also exhibit broad spectrum antifungal activity in conventional in vitro antifungal screening tests against human and animal pathogens such as the following medically important Candida species, Rhodatorula Rubra, Saccharomyces Cerevisiae, Torulopsis Glabrata, T. Menteagrophytes, Trichophyton Rubru , Microsporum Canis, Microsporum Gypseum, Epidermoph ton Floccosum, Monosporium Apiospermum and Glotrichum Candidum. Compounds of this invention also exhibited topical antifungal activity in in vivo tests. GENERAL SYNTHETIC PREPARATIONS The compounds of the present invention represented by formulas I and II may be synthesized utilizing the sequence of reaction illustrated in the following Methods. Method 1 In the formulas listed, in the Methods, Ar, Y, R ] _, LG and Q have exactly the same meaning as defined hereinabove. Compound III may be treated with dimethyloxosulfonium methylide [produced and used as described in "Reagents for Organic Synthesis" (Feiser and Feiser), Vol. 1, pages 314 to 316, J. Wiley and Sons, Inc. NY (1967)] to produce the oxirane of formula IV. See for example Example 1 herein. Compounds of formula III are readily prepared by treating the corresponding readily available phenylacyl halides with the appropriate azole anion in aprotic solvents such as dimethylformamide (hereinafter "DMF"). The oxirane of formula IV may be treated with aqueous mineral acid or alkali metal hydroxide to produce the 1,3-propanediol of formula V. The intermediates of formula II may be prepared by treating the 1,3-propanediols with the dialkylacetal or dialkylketal of formula VI in the presence of an acid in an organic solvent. Typical suitable acids include mineral acids such as HC1, HBr, H 2 S0^ and sulfonic acids such as p-toluene sulfonic acid. Solvents that may be used include aromatic hydrocarbons (ArH) such as benzene and toluene, halogenated hydrocarbons including carbon tetrachloride, chloroform and dichloroethane or mixed solvents such as aromatic hydrocarbons and lower alkanols (ROH) e.g. methanol, ethanol, propanol or butanol. Reflux temperatures and removal of alkanol, such as by use of a Dean-Stark trap facilitates the reaction. The aldehydes and ketones corresponding to formula VI may also be used in place of acetals and ketals VI in the reaction with V. The mixture of products cis-II and • trans-II may be separated by, for example, conventional chromatographic techniques well known to synthetic organic chemists or may be reacted with the anions represented by Q- anions, such as or -0-CH 2 -C≡C-CH -X, which may be prepared from the corresponding acids by reaction -with bases such as alkali metal hydride, e.g. NaH, or alkaline earth hydride, e.g. CaH or alkali metal amides, e.g. NaNH , in aprotic p. solvents such as DMF. The reaction of Q ~ with cis- II/trans-II may be carried out in aproptic solvents such as DMF or DMSO at temperatures of 20-100°C. The mixtures of cis-I and trans-I may be isolated and separated using standard separation techniques such as chromatography. The compounds of formula I have at least two asymmetric carbon atoms in their structures, namely those located at the 2- and 4-positions of the dioxolane ring, and consequently can exist in different sterochemically isomeric forms. The stereochemically isomeric forms of I and the pharmaceutically acceptable acid addition salts thereof are intended to be within the scope of this invention. The diastereomeric racemates of I, denoted as cis and trans forms respectively, according to the rules described in ' C.A., 76, Ind ^ ex Guide, Section IV, p. 85 (1972), may be obtained separately by conventional methods. Appropriate methods which may advantageously be employed therefore include, for example, selective crystallization and chromatography separation, e.g. column chromatography. Since the stereochemical configuration is already fixed in the intermediates (II) it is also possible to separate cis and trans forms at this or even an earlier stage, whereupon the corresponding forms of (I) may be derived therefrom in the previously indicated manner. The separation of cis and trans forms of such intermediates may be performed by conventional methods as described hereinabove for the separation of cis and trans forms of the compounds (I). It is evident that the cis and trans diastereomeric racemates may be further resolved into their optical isomers, cis(+), cis(-), trans(+), and trans (-) by the application of methodologies known to those skilled in the art. Methods 2 and 3 illustrate sequences of reaction useful for preparation of the 1,3-propane diols of formula V Method 2 θ i The readily available a-styrene compounds of formula VII may be treated with peracid (RCO3H) to produce the oxirane of formula VIII. Peracids such as metachloroperbenzoic acid or peracetic acid may be used in halogenated solvents such as chloroform or methylene chloride. The oxirane VIII may be treated with hindered bases such as alkali metal di(loweralkyl)amides to produce the a-aryl-a-methylene ethanols of formula IX. Hindered bases such as alkali metal diloweralkylamides may be readily formed in situ by reaction of strong bases such as n-butyl lithium with the corresponding di (loweralkyl) amine. Reaction of IX with peracids produces the 1-hydroxymethyl-l-aryl oxiranes of formula X. Treatment of the oxiranes of formula X with azole anions in aprotic solvents such as DMF provides 1,3-propanediols of formula V. Method 3 provides an alternative synthesis of 1,3-propanediols V starting from the methyl aryl ketones of formula XI which are readily available or may be prepared by methods well-known to the skilled synthetic organic chemist. Method 3 H (xv) The aryl methyl ketone XI may be treated with trimethylsilylcyanide and zinc iodide at 50°C to produce compounds of formula XII to prolonged acid-catalyzed alcoholoysis to produce the ethyl α-aryl-α-methylacetate of formula XIII. Reaction of XIII with peracid provides the oxirane XIV which when treated with azole anion gives the ethyl α-aryl-α-hydroxypropanoate of formula XV. Hydride reduction of XV with, for example, LIAIH4 in ether provides the 1,3-propane diols of formula V. The following Examples illustrate additional methods for the preparation of compounds of this invention. Method 4 CIS- (I) + TRANS-(I) As depicted in Method 4, the compounds of formula (I) may also be prepared by treatment of dialkylacetal of formula (VI) where R is an alkyl, first with the anions Q~ as defined above to provide compounds of the type (XVI). Acetal exchange reaction of 1,3- propanediols (V) with (XVI) in the presence of an acid in an organic solvent [as previously described for the reaction between (VI) and (V)] provides a mixture of cis- (I) and trans-(I) which may be separated by chromatography. Method 5 CIS- (XVIII) + TRANS- (XVIII) CIS-(XX )+TRANS- ( XX ) CIS-( IX)÷TRftMS- XIX ) CIS-(I) M-LG TRANS-(I) Method 5 shows an alternative approach in which the 1,3-propanediols (V) may be treated with a dialkylacetal of the formula (XVII) [as in Method 4] to provide a 1,3-dioxolane of the formula (XVIII) as a cis- and trans-isomer mixture. Removal of the benzyl protecting group (e.g. by hydrogenolysis) followed by treatment of the alcohols (XIX) with NaH in dimethylformamide yields the alkoxides (XX) . Reaction of (XX) with an electrophile such as M-LG leads to a mixture of cis-(I) and trans-(I) , where M PREPARATIVE EXAMPLE 1 A. PREPRATION OF 2-(4-CHLOROPHENYL)-3-(1H-1,2,4-TRIAZOL- l-YL)-l,2-PROPA EDIOL To a stirred solution of _p_-chlorophenacyl-lH- 1,2,4-triazole (10.3 g, 40 mmol) in 65 mL of toluene and 65 mL of 20% w/w NaOH at 60°C, add trimethylsulfoxonium iodide (8.84 g, 40 mmol) and cetrimide (0.39 g) . Continue to stir the so-formed mixture at 60°C for 1.5 h. Separate the toluene layer, add 20 mL of H 2 0 to the aqueous layer and extract with two 100 mL portions of ethyl acetate (EtOAc) . Combine both the toluene and EtOAc layers, dry over MgSO^, filter, and evaporate off the solvents. Add 100 mL of acetone, 6 mL of concen¬ trated H SO^, 20 mL of H 2 0, and reflux for 3 hr. Evaporate the acetone at 45°C under reduced pressure. Add 20 mL of sat. sodium bicarbonate and extract with two 100 mL portions of EtOAc. Wash the combined EtOAc layers with sat. brine, dry over MgS0 4 , and evaporate the EtOAc to obtain a syrup. Chromatograph the syrup on 300 g of silica gel using 5% MeOH/CH 2 ci 2 as the e uent under flash chromatography conditions to obtain 3.63 g of the title product. B. (±)CIS AND TRANS-l-[[2-(BROMOMETHYL)-4-(4- CHLOROPHENYL)-1,3-DIOXOLAN-4-YL]METHYL]-1H-1,2,4-TRIAZOLE To a suspension of 2-(4-chlorophenyl)-3-(1H- 1,2,4-triazol-l-yl)-1,2-propanediol of Step A (3 g, 0.012 mol) in 150 mL of dry toluene, add _p_-toluene sulfonic acid monohydrate (2.55 g, 0.013 mol), bromoacetaldehyde diethylacetal (2.25 L, 0.015 mol), and reflux using a Dean-Stark trap. After 5 hr, cool to room temperature. Add ΞtoAc (100 mL) and wash with 100 mL of sat. NaHC0 3 . Dry over MgSO^, filter, and evaporate off the solvents to obtain 5.2 g of crude product. Chromatograph the diastereomeric mixture on 200 g of silica gel using 50% EtOAc/hexane as the eluent to obtain 1.52 g of cis-isomer followed by 2.42g of the trans-isomer of the title compound. EXAMPLE 1 (±)CIS OR TRANS-l-ACETYL-4[4-[ [4-(4-CHL0RPHENY )-4- (1H- 1,2,4-TRIAZ0L-1-YLMΞTHYL)-1,3-DI0X0LAN-2-YL]METHOXY] PHE YL]PIPERAZINE To a suspension of NaH (50%) dispersion (0.38g, 8 mmol), in 30 mL of DMF, add 1- (4-hydroxyphenyl)-4- acetylpiperazine (1.76 g, 8 mmol). After stirring lhr at room temperature add, dropwise, the product of Preparative Example 1 (2.4 g, 6.7 mmol) in 10 mL of DMF. After stirring 18hr, add 50 mL of sat. brine and extract with two 100 mL portions of EtOAc. Dry the combined EtOAc layers over MgS0 4 , filter, and evaporate off the solvents at 60°C and reduced pressure. Chromatograph the resulting oil on 200 g of silica gel using 3% MeOH/CH 2 Cl 2 as the eluent, under flash chromatography conditions, to obtain 1.45g of the title compound. PREPARATIVE EXAMPLE 2 PREPARATION OF 2-(4-CHLOROPHENYL)-3-(lH-1,2,4-TRIAZOL-1- , YL)-l,2-PROPANEDIOL a) 2-(4-CHLOROPHENYL)-2-METHYLOXIRA E To a stirred and cooled solution (0-5°C) of 1- σhloro-4-methylethenyl) benzene (15.2g) in chloroform (350 mL) , add m-chloroperbenzoiσ acid (21.6g). Stir the reaction mixture for lhr by which time all starting material will have reacted to give virtually a single product. Remove the m-chlorobenzoic acid formed by washing the chloroform solution with aqueous NaHCO j followed by water (150 mL) . Dry the chloroform solution (Na 2 S0 4 ) and evaporate to dryness to provide a colorless oil. Distill the oil _in_ vacuo (1 mm) and collect fractions distilling between 65-72° to give the title compound (8.8g). b) PREPARATION OF 4-CHLORO-g-METHYLENE BENZENE ETHANOL To a cooled solution (5°C) of diethylamine (3.2g) in dry diethyl ether (100 mL) add (under 2 atmosphere) n-butyl lithium (29 L, 1.55M solution in n- hexane) . Maintain the temperature between 5 and 10°C during the addition of n-butyl lithium. Add the epoxide of Preparative Example 2a, (5g) via syringe as a solution in dry diethyl ether (25 mL). Allow the reaction mixture to warm to ambient temperature and then reflux for 3hr. After cooling to 0-5°C, add water (100 mL) carefully, separate the ether layer and wash with IN HCl, water, and aqueous NaHCθ3. Dry the ether layer (NaS0 4 ), and evaporate the ether in vacuo, to give colourless oil (3.1g). Chromatograph the oil on silica gel to provide the title compound. C) PREPARATION OF 2-(4-CHLOROPHENYL)OXIRANEMETHANOL To a solution of the compound prepared in Preparative Example 2(b), (1.05g) in chloroform (50 mL) add, with cooling, (ice bath) a solution of m- chloroperbenzoic acid (1.2g) in chloroform. Stir the chloroform solution for 4 hr. Wash the chloroform solution, successively, with aqueous 10% NaHSθ3 (to remove excess per acid) followed by aqueous aHC03 and finally with water. Dry (Na 2 S0 4 ) the chloroform solution. Remove the chloroform in vacuo to provide the title compound as a colorless oil (0.83g). Use the title compound without any further purification in the next reaction. d) PREPARATION OF 2-(4-CHLOROPHENYL)-3- (1H-1,2,4- TRIAZOL-1-YL)-1,2-PROPANEDIOL To a suspension of NaH (0.09g) in dry DMF (20 mL) , add 1,2,4-triazole (0.27g), and stir the so-formed mixture for 1 hr. Add a solution of the epoxy alcohol prepared in Preparative Example 2(c), (0.8g) in dry DMF (15 mL) . Heat the reaction mixture (bath temperature 70- 75°) for 2V 2 hours. Remove most of the DMF in vacuo and distribute the residue between water (60 mL) and chloroform (100 mL) . Separate the chloroform layer, wash with water, dry over Na- > S0 4 and evaporate the solution to provide the title compound as a crystalline solid (0.7g). This diol compound was identical in all respects to the product derived from acid hydrolysis of l-[[2-(4- chlorophenyl) -oxiranyl]methyl]-1H-1,2,4-triazole. PREPARATIVE EXAMPLE 3 PREPARATION OF 2-(2,4-DICHL0R0PHENYL-3-(1H-IMIDAZ0L-1- YL)-1,2-PR0PANEDI0L _ a) 2,4-DICHLOROACETOPHENONE CYANOHYDRIN-O- TRIMETHYLSILYLETHER Heat a mixture of 2,4-dichloroacetophenone (48.5g) trimethylsilylcyanide (25.2g) and zinc iodide (0.09g) at 50°C for 3 hours. Allow the reaction mixture to stand overnight at room temperature. Isolate the title compound as an oil and use it in the next reaction without further purification. b) ETHYL 2,4-DICHLORO-α-METHYLENE BENZENE ACETATE AND 2,4-DICHLORO-ce-METHYLENE BENZENEACETAMIDE Add the cyanohydrin prepared in Preparative Example 3(a), (75g) dropwise to a solution of con¬ centrated H S0 4 containing 4% SO3 (60 mL) and 250 mg of copper powder at 40°C. During the addition, maintain the reaction temperature between 80-85°C (water bath). After all cyanohydrin is added, raise the temperature to 100°C and heat the reaction mixture at this temperature for 30 min. Cool the reaction mixture to 90°C and carefully treat with water (26 mL) and ethanol (60 mL). Heat the mixture at 100°C for 15 hrs, cool and dilute with ice cold water (200 mL). Separate the organic layer, dilute with CH 2 C1 2 (100 mL) and wash with brine (100 mL). 'Extract the combined aqueous phase containing solids twice with EtOAc (400 mL), wash with saturated NaHCθ3, water (100 mL each) and dry over Na 2 S0 4 . Evaporate the EtOAc in vacuo to give an oil containing a crystalline solid. Dilute this residue with a small volume of CH 2 Cl 2 -separate crystals of α-(2,4-dichlorophenyl)-α- methyleneacetamide and collect by filtration (26g). Combine the filtrate with the organic layer obtained earlier and chromatograph the concentrate on silica gel (300g) to provide ethyl-α-(2, 4-dichlorophenyl)- - methyleneacetate. C) ETHYL 2-(2,4-DICHLOROPHENYL)OXIRANECARBOXYLATE Treat a stirred solution of the styrylester of Preparative Example 3(b), (20.6g) in chloroform (500 L) with m-chloroperbenzoic acid (16.5g) and heat the mixture at reflux overnight. Cool the reaction mixture, treat with 10% aqueous sodium bisulfite solution to remove excess peracid, wash with aqueous aHC03, water and dry over Na 2 S0 4 . Evaporate CHCI3 in vacuo to give an oil. Chromatograph the oil on silica gel to give the title compound (10.6g) and unchanged styryl ester starting material. d) ETHYL α-(2,4-DICHL0R0PHENYL)-α-HYDR0XY-lH_-IMIDAZ0LE- 1-PROPANOATE Treat a solution of the compound prepared in Preparative Example 3(c), (8g) in dry DMF (100 mL) with i idazole (2.1g) and heat the mixture (bath temp. 140- 150°C) for 4hr. Remove the DMF in vacuo. Take up the residue in water and extract with EtOAc. Dry EtOAc extract with Na S0 and evaporate to dryness to provide a crystalline residue. Recrystallize the residue from EtOAc-_n-he ane to give 4.2g of the title compound, m.p. 177-179°C. e) 2-(4-CHLOROPHENYL)-3-(1H_-IMIDAZ0L-1-YL)-1,2- PROPANEDIOL Add a solution of the hydroxy-ester of Preparative Example 3(d), (4.1g) in dry tetrahydrofuran (200 mL) dropwise, with stirring, to a suspension of LiAlH 4 (1.5g) in tetrahydrofuran (300 mL). Heat the reaction mixture at reflux for 18hr and cool (ice bath). Carefully add EtoAc to destroy excess LiAlH 4 and then add 10% Na-K-tartarate (50 mL) . Evaporate most of the tetrahydrofuran in vacuo and extract the remaining aqueous suspension with EtOAc. Wash the EtOAc extract with saturated NaCl solution, dry Na 2 S0 4 and evaporate to dryness to provide 4.2 g of a crystalline solid, Recrystallize the solid from EtOAc to yield the title compound, m.p. 146-148°C. PREPARATIVE EXAMPLE 4 A. 2-(2,4-DICHLOROPHENYL)-3-(lH-l-IMIDAZOL-l-YL)-1,2- PROPANEDIOL Heat a solution of l-[ [2-(2,4-dichlorophenyl) oxiranyl]methyl]-lH-imidazole (12g) [prepared in accordance with a procedure described in British patent application No. 2,078,719A, published 13 Jan. 1982] in 90% formic acid (250 mL) at reflux for 15hr. Evaporate the reaction almost to dryness _iιι vacuo, take up the residue in water (100 mL) and carefully basify with aqueous K 2 Cθ3. Add sufficient methanol (100 mL) to dissolve the contents, and heat the mixture (bath temp. 100°C) for lhr. Evaporate the solvents in " vacuo and isolate the product by extraction with CHCI3. Concentrate chloroform extract to provide colorless "crystals of the title compound (lOg) , m.p. 127-128°C. B. (±) CIS AND TRANS-l-[[2-(BROMOMETHYL)-4-(2,4- DICHLOROPHENYL)-1,3-DI0X0LAN-4-YL]METHYL]-1H-IMIDAZOLE To a well stirred suspension of 2-(2,4- dichlorophenyl)-3-(lH-imidazol-1-yl)-1,2-propanediol prepared in Step A above (4g) in toluene (200 mL) and n- butanol (5 mL) , add successively p-toluenesulfonic acid monohydrate (2.9g) and bromoacetaldehyde diethylacetal (2.7g). Heat the mixture gently at reflux and distill the ethanol (5hr) . Add additional toluene (100 L) and heat the mixture at reflux gently overnight. Cool the reaction mixture, dilute with EtOAc (100 mL) and wash with aqueous NaHC0 3 . Dry the organic phase with Na 2 S0 4 and evaporate to dryness _ n vacuo to provide a gummy product. Chromatograph the gummy product on silica gel (200g) using 20% n-hexane/EtO c as eluent to give the cis-isomer (less polar) 1.38g and the trans-isomer (more polar) 2.36g of the title compounds. EXAMPLE 2 (±)CIS-l-ACETYL-4-[4-[ [4- (2,4-DICHL0R0PHENYL)-4- (1H- IMIDAZOL-l-YLMETHYL)-1,3-DI0X0LAN-2-YL] ETHOXY]PHENYL] PIPERAZINE To a stirred suspension of NaH (60% dispersion in mineral oil, 0.196g) in dry DMF (10 mL) under argon atmosphere, add l-acetyl-4-(4-hydroxyphenyl)-piperazine (1.08g). Heat the mixture (bath temp. 50°C) for_lhr until H 2 evolution has ceased. To the so formed sodium phenoxide, add the cis isomer prepared in Preparative Example 1(b) (1.28g, dried by azeotropic distillation of a benzene solution) in dry DMF (2 mL) . Heat the mixture (bath temp. 60°) with stirring for 20 hr. Cool, add water (10 mL) and extract the reaction product with EtOAc (4 x 50 mL) . Wash the EtOAc extracts with water (10 mL) and dry with Na 2 S0 4 . Evaporate the organic extract in vacuo to give the crude product as a brown gum. Chromatograph the brown gum on silica gel (lOOg) using 1- 10% methanol/σhloroform as eluent to give the title compound as an amorphous solid (0.8968g). EXAMPLE 3 (±)TRANS-l-ACETYL-4-[4-[ [4-(2, -DICHLOROPHENYL)-4- (IE) IMIDAZOL-1-YLMETHYL)-1,3-DIOXLAN-2-YL] ETHOXY]PHENYL] P Follow the procedure of Example 2, but add 1.4g of (±) trans-l-[[2-(bromomethyl)-4-(2,4-dichlorophenyl)- l,3-dioxolan-4-yl]methyl]-lH_-imidazole prepared in Preparative Example 4 to 1.24g of l-acetyl-4-(4- hydroxyphenyl) piperazine. Chromatograph on silica gel, to give 0.6g-of the title compound as an amorphous solid. PREPARATIVE EXAMPLE 5 (±)CIS AND (±)-TRANS-l-[[2-(BROMOMΞTHYL)-4-(2,4- DICHLOROPHENYL)-2-METHYL-l,3-DIOXLAN-4-YL] ETHYL]-1H- IMIDAZOLE To a well- stirred suspension of 2-(2,4- dichlorophenyl)-3-(lH-imidazol-1-yl)-1,2-propanediol ( 3 . 4g) , in toluene ( 500 mL) and n-butanol (4 mL) , add successively with p-toluenesulfonic acid monohydrate (2.47g) and 2,2-dimethoxybromopropane (3.2g). Heat the mixture gently at reflux for 3βhr. Treat the reaction mixture in a manner described in Preparative Example 4 to give a mixture of the title compounds. Chromatograph on silica gel to provide the title compound, cis-isomer (less polar) m.p. 145-147 e C, and trans-isomer (more polar) m.p. 128-130°C. EXAMPLE 4 (±)C_IS_-l-ACETYL-4[4-[ [4- (2,4-DICHLOROPHENYL)-4-C1H- IMIDAZOL-l-YLMETHYL)-2-METHYL-l,3-DIOXOLAN-2- YL]METHOXY]PHENYL]PIPERAZINE To a stirred solution of l-acetyl-4-(4- hydroxyphenyl) -piperazine (0.448g) in DMF (5 mL) add NaH [(60%) dispersion; 0.085g] under an argon atmosphere. Heat the mixture (bath temp. 50°C) for lhr until H 2 evolution had ceased. Add successively {±)cis-l-[ [2- (bromomethyl)-4-(2,4-dichlorophenyl)-2-methyl-l,3- dioxolan-4-yl]methyl]-lH-imidazole (0.5g) prepared in Example 2 in one portion and a suspension of 18-crown-6- ether (0.326g) in acetonitrile and heat the reaction (bath temp. 80°C) for 5 days. Treat the reaction mixture in a manner described in Example 2 to give a crude solid. Chromatograph on silica gel to provide the title compound as an amorphous solid (0.36g). PREPARATIVE EXAMPLE 6 A. 2- (2,4-DIFLUOROPHENYL)-3-(1H-1,2,4-TRIAZOL-l-YL)-1,2- PROPANEDIOL Heat a solution of l-[ [2-(2,4-difluorophenyl) - oxiranyl]methyl]-lH-l,2,4-triazole (57g) (prepared as described in Example 2(c) of British patent application 2,099,818A, published 15 Dec. 1982) in formic acid (500 mL) at reflux overnight. Remove the solvent in vacuo at 85°C. Dilute the residue with ice water (1 liter) and basify carefully with 10% K 2 Cθ3 solution. Dilute with MeOH (500 mL) and heat on steam bath (1.5hr). Remove the MeOH in vacuo and extract the remaining aqueous solution with EtOAc (2x500 mL) . Wash the EtOAc layer with H 2 0 and dry it over MgS0 and evaporate it to dryness to give 49.7g of the title compound as a solid, m.p. 130-132°C. B. (±)CIS AND TRANS-1-[ [2-(BROMOMETHYL)-4-(2,4- DIFLUOROPHENYL)-l,3-DIOXOLAN-4-YL]METHYL]-lH-l,2,4- TRIAZOLE To a stirred suspension of 15g of the compound prepared in Preparative Example 6 Step A above in toluene (600 mL) , add successively with p-toluene sulfonic acid monohydrate (12-.6g) and bromoacetaldehyde diethyl acetal (14.7 cc) and heat the so-formed mixture at reflux overnight with azeotropic removal of water (Dean-Start trap) . After 16hr, cool and then wash the reaction mixture with sat. NaHCθ sol. (300 mL) and extract the aqueous phase with EtOAc (300 L) . Combine organic phases arid wash them with H 2 0 (200 mL) . Dry with Na 2 S0 4 and evaporate to dryness in vacuo. - Chromatograph the residue on silica gel to obtain 3.9g of the less polar cis isomer, m.p. 75-77°C and 7.2g of the more polar trans isomer, m.p. 81-84°C of the title compound. EXAMPLE 5 A. METHYL(±)CIS-6-[ [ [4- (2,4-DIFLUOROPHENYL)-4-(lH-1, 2,4- TRIAZOL-1-YLMETHYL)-1,3-DI0X0LAN-2- YL]METHYL]THIO]HEXANOATE Add sodium hydride 260 mg to a solution of methyl 5-mercapto-hexanoate 900 mg in dry DMF (50 mL) at room temperature. After V hour, add the less polar cis isomer (2.3g)-of Preparative Example 6 in DMF (15 mL) and heat at 80°C (bath temperature) for 2h. Add the mixture to cold water (600 cc) and extract with EtOAc (400 mL) . Wash the EtOAc layer with brine * (200 mL) , dry over NaS0 4 and evaporate to give an oil. Chromatograph the oil on silica gel to give 2.1g of the title compound as an oil. B. (±)CIS-6[ [ [4-(2,4-DIFLUOROPHENYL)-4-(lH-l,2,4- TRIAZOLE-l-YLMETHYL)l,3-DIOXOLAN-2-. YL]METHYL]THIO]HEXANOIC ACID .-C-O-H Heat a solution of the compound prepared in Step A above (100 mg) in Claisen alkali (5 mL) [Claisen, Ann., 418, page 96 (1919)] on a steam bath for V hour. Remove the methanol _iιτ vacuo, dilute the solution with water and acidify it carefully with cone. H 2 S0 . Filter the precipitate so-formed, wash with H 2 0 and dry in a vacuum oven at 50°C to provides a solid (75mg) . Recrystallize the solid from EtOAc:hexanes to give the title compound, m.p. 126-129 β C. EXAMPLE 6 (±)CIS-l,1'-[4-(2,4-DIFLUOROPHENYL)-1,3-DIOXOLAN-2,4- DIYLBIS(METHYLENE) ]BIS-[1H-1,2,4-TRIAZOLE] Add sodium hydride (120 mg, 60% oil disp.) to a solution of triazole (210 mg) in dry DMF (10 mL) at room temperature and stir V2 hour. Add a solution of the less polar isomer of Preparative Example 6 (900 rag) in DMF (10 mL) and heat the reaction for lhr at 80°C. Remove DMF in vacuo, take up the residue in water (50 mL) and extract with EtOAc (100 mL) . Wash the EtOAc extract with brine (100 mL) dry over Na 2 S0 and evaporate the solvent. Recrystallize the residue from EtOAc.hexanes to give 520 mg of the title compound as a solid, m.p. 168-169°C. EXAMPLE 7 (±)CIS.-4-[4-[4-[4-[ [4- (2,4-DIFLUOROPHENYL)-4-(lH-1,2,4- TRIAZOL-1-YLMETHYL)-1,3-DIOXOLAN-2-YL]METHOXY]PHENYL]-1- PIPERAZINYL]PHENYL]-2,4-DIHYDRO-2-(1-METHYLPROPYL)-3H.- l,2,4-TRIAZOL-3-ONE Add,sodium hydride (120 mg; oil disp.) to a solution of 2,4-dihydro-4-[4-[4-(4-hydroxyphenyl)-1- piperazinyl]-phenyl]-2-(1-methylpropyl)-31^-1,2,3-triazol- 3-one (l.Og) in dry DMF (20 mL) . Warm the mixture at 30- 35°C for V hour. Add a solution of (±)cis-l-[ [2- (bromomethyl)-4-(2,4-difluorophenyl)-1,3-dioxolan-4- yl]methyl]-1H-1,2,4-triazole prepared in Preparative Example 6 (1.2g) in dry DMF (10 mL) and heat (bath temp. 90°C) for 3h. Cool, pour into (200 mL) ice water, extract with EtOAc (2 x 200 mL) and wash with water (75 mL) . " Dry the EtOAc extract with Na 2 S0 4 and evaporate to dryness in vacuo. Chromatograph the residue so obtained on silica gel to provide (l.Og) product of the title compound, m.p. 158-161°C. EXAMPLE 8 (±)CIS-l-ACETYL-4-[4[[4-(2,4-DIFLϋOROPHENYL)-4-(1H-1,2,4- TRIAZOL-l-YLMETHYL)-1,3-DI0X0LAN-2-YL]METHOXYl PHENYL]PIPERAZINE To a stirred solution of l-acetyl-4-(4- hydroxyphenyl)-piperazine (2.56g) in dry DMF (30 mL) , add sodium hydride (660 mg 60% or disp.) . Maintain at room temperature during addition. After lhr, add (±)-cis-l- [ [2-(bromomethyl)-4-(2,4-dij:luorophenyl)-l,3-dioxolan-4- yl]methyl]-1H-1,2,4-t iazole prepared in Example 7 (3.'0g) dissolved in dry DMF (15 mL) and heat the solution (bath temp. 85°C) for 2hr. Remove most of the DMF in vacuo and take up the residue in water (400 mL) extract with EtOAc (2x200 cc) and wash with 10% 2 C0 3 , then water. Dry the EtOAc extract over Na 2 S0 4 and evaporate to give a residue. Chromatograph the residue on silica gel to provide the title compound (2.3g) m.p. 158-160°C. EXAMPLE 9 (±)CIS-1-14-[ [4- (2,4-DIFLϋOROPHENYL)4-(lH-1,2,4-TRIAZOL- 1-YLMETHYL)-1,3-DIOXOLAN-2-YL]METHOXY]PHENYL]-4-(1- METHYLETHYL)PIPERAZINE Add NaH (320 mg, 60% on disp.) to a dispersion of 1-(4-hydroxyphenyl)-4-(1-methylethyl)piperazine (1.7g) in dry DMF (20 mL) and stir for lhr. Add a solution of (±)cis-l-[ [2-(bromomethyl)-4-(2,4-difluorophenyl)-1,3- dioxolan-4-yl]methyl]-1H-1,2,4-triazole prepared in Preparative Example 6 (2g) in dry DMF (10 mL) at room temperature and heat the reaction for lhr at 100°C. Cool and remove the DMF in vacuo at 45°C. Dilute the residue with water (100 mL) and extract with EtOAc (2x150 mL) . Wash with water, dry over Na 2 S0 4 and evaporate the organic solvent, to give an oil. Chromatograph the oil on silica gel to give the title compound (610 mg) m.p. 125-127°C. ΞXAMPLE 10 (±)-CIS AND TRANS-DERIVATIVES OF [4-(2,4-DICHLOROPHENYL) l,3-DIOXOLAN-4-YL]METHYL]-lH-l,2,4-TRIAZOLES OF THE cxs traris a) Follow the procedure of Example 2 except substitute for l-(p-hydroxyphenyl)-ρiρerazine-N-acetate equivalent quantities of the compounds listed under HQ HQ HO - -<C ^K>— N> V- C-C 6 H 5 0 II HO-CH 2 -CΞ.C-CH 2 -C-CH 3 0 HO-CH 2 -C=.C-CH 2 -N N-C-CH- to produce the corresponding (±) cis compounds of above formula with the appropriate Q group. b) Follow the procedure of Example 3 except substitute for l-acetyl-4-(4-hydroxyphenyl)-piperazine equivalent quantities of the compounds of formula ' s HQ in Example 20 (a) to provide the corresponding (±) trans compounds with the appropriate Q group. EXAMPLE 11 PREPARATION OF (i) CIS_ AND TRANS-4- ( 2 , 4-D IFLUOROPHENYL ) - 1 , 3-D IOXOLAN-4-YL] METHYL] (1H-1 ,2 , 4-TRIAZOLES OF THE FORMULAS : trans a) Follow the procedure of Example 8 except substitute for l-acetyl-4-(4-hydroxyphenyl)piperazine equivalent quantities of the following compounds listed under HQ HQ -53- H 2 N—/ - -NN II (Y=CH;N) 2N - ^ O^ -0 ^ Hj - V-N N-C-CH(CH 3 ) 2 ■oo H,N •-O(' λ )-N; SO. OMe OMe ^ H2N - ^ y ■ C C H. OMe OMe H 2 N-CH 2 -C=C-CH 2 ^C-CH to provide the corresponding (±)-cis compounds of the above formula with the appropriate Q group * b) Follow the procedure of Example 8 but substitute for the -hcis triazole an equivalent quantity of the (±)trans triazole from Preparative Example 4 and for the 1-acetyl- 4-(4-hydroxyphenyl)piperazine equivalent quantities of the compounds of formula HQ listed in Example 11(a) to provide the corresponding (±) trans compounds of the above formula with the appropriate Q group. EXAMPLE 12 (±) -CIS AND (±) -TRANS DERIVATIVES OF 4 (4-CHLOROPHENYL) - l , 3-D IOXOLAN- 4-YL] METHYL-lH~TRIAZOLES .OF THE FORMULAS : trans CIS a) Follow the procedure of Example 1 except substitute for l-(4-hydroxylphenyl-N-acetate equivalent quantities of the following compounds listed under HQ HQ - -NN 1 I (Y=CH;N) HS . '' " ^- N S HS-/' '>-N SO. 0 HS-CH 2 -C H .C-CH 2 -N^ N-C-CH 3 to produce the corresponding ±(cis) compounds of the above formulas with the appropriate Q group. b) Follow the procedure of Example 1 except substitute for the (±)cis triazoles, e.g. an equivalent of the (±)- l-[ [2-(bromomethyl)-4-(4-chlorophenyl)-l,3-dioxolan-4- yl]methyl]-lH-l,2,4-triazole of Preparative Example 1 to obtain the corresponding (±) trans compounds of the above formula with the appropriate Q group. EXAMPLE 13 The following are typical pharmaceutical formulations containing as the active ingredient (designated "Drug") a compound of formula I. FORMULATION 1 Tablet 125.00 mg. tab. Drug 125.00 ' mg. Polyethylene glycol 6000 100.00 mg. Sodium lauryl sulfate 6.25 mg. Corn starch 30.00 mg. Lactose, anhydrous 87.25 mg. Magnesium stearate 1.50 mg. Procedure: Heat polyethylene glycol 6000 to 70-80°C. Mix the drug, sodium lauryl sulfate, corn starch, and lactose into the liquid and allow the mixture to cool. Pass the solidified mixture through a mill. Blend granules with magnesium stearate and compress into tablets. FORMULATION 2 Capsule 250 mg. tab. Drug 250.00 mg. Lactose, anhydrous 100.00 mg. Corn starch 50.00 mg. Microcrystalline cellulose 95.00 mg. Magnesium stearate 5.00 mg. Procedure: Mix the first four ingredients in a suitable mixer for 10-15 minutes. Add the magnesium stearate and mix for 1-3 minutes. Fill the mixture into suitable two- piece hard gelatin capsules using an encapsulating machine.

Claims: CLAIMS: 1. A compound having structural formula I: wherein Ar is thienyl, pyridyl, biphenyl, phenyl or phenyl substituted by one or more of halo, nitro, cyano, lower alkyl, lower alkoxy or perhalo(lower)alkyl; Y is CH or N; .- -W-CH 2 C ΞC-CH 2 X or -W-CH (R 3 ) - (CH 2 ) p -C0 2 R 4 W is -NR 5 -, -0- or -S (0 ) n - ; X is N0 , NRgR 7 or COR g ; R-^ r R , R3 and R are independently hydrogen or lower alkyl; R5 is hydrogen, lower alkyl or (C 2 -Cg)alkanoyl; Rg and R7 are independently hydrogen lower alkyl, phenyl or phenyl substituted by one or more of halo, perhalo(lower)alkyl, (C 2 -Cg)alkanoyl, lower alkyl, lower alkoxy, or 2-(lower)alkyl-3-oxo-l,2, 4- triazol-4-yl, or Rg and R7 taken together with the nitrogen atom in NR R 7 form an unsubstituted or substi¬ tuted five or six member heterocyclyl ring containing one to four hetero atoms chosen from N, 0 and S(0) n , said heterocyclyl substituents being (C -Cg)alkanoyl, lower alkyl, phenyl or phenyl substituted by one or more of halo, perhalo lower alkyl, (C 2 -Cg)alkanoyl, lower alkyl, lower alkoxy, or 2-loweralkyl-3-oxo-l,2,4-triazol-4-yl; Rg is a lower alkyl, lower alkoxy, -NR- j _R 2 , phenyl or phenyl substituted by one or more of halo, perhalo lower alkyl, lower alkoxy, nitro, cyano, (C 2 -Cg)alkanoyl; p is 0, 1, 2, 3, 4 or 5; n is 0, 1 or 2; and the stereochemical isomers thereof in racemic or optically active form; or a pharmaceutically acceptable salt thereof. as defined in claim 1 above wherein 3. A compound of either claim 1 or claim 2 above wherein wherein Z is hydrogen, (C 2 -Cg) alkanoyl, lower alkyl, lower alkoxy, perhalo lower alkyl, phenyl or phenyl substituted by one or more of cyano, nitro, halo, perhalo lower alkyl, lower alkyl, lower alkoxy, (C 2 ~Cg)alkanoyl or 2-lower alkyl-3-oxo-l,2,4-triazol-4-yl. 4. A compound of claim 1 above wherein Q is S (0) n -CH(R 3 )-(CH 2 ) p -C0 2 R 4 . 5. A compound of any of claims 1-3 above 1 which is: (±)cis-l-acetyl-4-[4-[ [4-(4-chlorophenyl) -4-(1H- 1,2,4-triazol-l-ylmethyl) -1,3-dioxolan-2-yl]methoxy] phenyl]piperazine; (±) trans-l-acetyl-4-[4-[ [4- (4-chlorophenyl)-4- (lH-l,2,4-triazol-l-ylmethyl)-l,3-dioxolan-2-yl]methoxy] phenyl]piperazine; (±)cis-4-[4-[4-[4-[ [4-(2,4-difluorophenyl)-4- (1H-1,2,4-triazol-l-ylmethyl)-1,3-dioxolan-2yl]methoxy] phenyl] l-piperazinyl]phenyl]-2,4-dihydro-2-(l- methylpropyl)-3H-l,2,4-triazole-3-one; (±)cis-1-[4-[ [4-(2,4-difluorophenyl)-4-(1H- 1,2,4-triazol-l-ylmethyl)-l,3-dioxolan-2-yl]methoxy] phenyl]-4-(1-methylethyl) piperazine; methyl(±)cis-6-[[[4-(2,4-difluorophenyl)-4-(1H- 1,2,4-triazol-l-ylmeth l)-1,3-dioxolan-2- 1] ethy1] thio]hexanoate; (±)cis-6-[ [ [4-(2,4-difluorophenyl)-4-(1H-1,2,4- triazol-1-ylmethyl)-1,3- ioxolan-2-yl]methyl] thio] hexanoic acid; (■b)cis-l-acetyl-4-[4-[ [4-(2,4-dichlorophenyl)- 4-(lH-imidazol-l-ylmethyl)-l,3-dioxolan-2-yl]methoxy] phenyl]piperazine; t (±)cis-l-acetyl-4-[4-[ [4-(2,4-difluorophenyl)- 4-(1H-1,2,4-triazol-l-ylmethyl)-1,3-dioxolan-2-yl] methoxy]phenyl]piperazine; (±)trans-l-acetyl-4[4-[[4-(2,4-dichlorophenyl)- 4-(IH-imidazol-l-ylmethyl)-1,3-dioxolan-2-yl] ethoxy] phenyl]piperazine; or (±)cis-l-acetyl-4-[4-[ [4-(2,4-dichloroρhenyl)- 4-(lH-imidazol-l-ylmethyl)-2-meth l-l,3-dioxolan-2- yl]methoxy]phenyl]piperazine. 6. A compound as defined in any one of claims 1-5 above in combination with a pharmaceutically acceptable carrier. 7. A method for making a pharmaceutical composition as defined in any of claims 1-6 above comprising mixing a compound of formula I with a pharmaceutically acceptable carrier. 8. A process for producing a compound having structural formula I in claim 1 characterized by: (a) reacting a compound of the formula with an anion Q 9 in an aprotic solvent where LG is a leaving group; (b) reacting a compound of the formula with a compound of the formula (R0) -Cf-CH-Q where R is an alkyl; and (c) reacting a compound of the formula with a compound of the formula M-LG where M is \\—X 9. The use of a compound as claimed in any of claims 1-9 above claim 1 for the preparation of a pharmaceutical composition for hypoproliferative skin disease, anti-inflammatory, anti-fungal, anti-allergy and/or immunomodulating application. 10. A method of treating and/or preventing fungal infections, hyperproliferative skin disease, inflammation, allergy and/or immune system disorders comprising administering an effective amount of a compound of formula I as defined in any of claims 1 to 9 above. 11. A compound represented by formula II or formula XX: (II) CIS -(XX )+TBA NS -" tXX) wherein Ar is thienyl, pyridyl, biphenyl, phenyl or phenyl substituted by one or more of halo, nitro, cyano, lower alkyl, lower alkoxy or perhalo lower alk l; Y is CH or N; LG is a leaving group; R^ and R 2 are hydrogen or lower alkyl; and the stereochemical isomers thereof in racemic or optically active form.

Inventors: COOPER ALAN B, GANGULY ASHIT K, GIRIJAVALLABHAN VIYOOR M, GUZIK HENRY, SAKSENA ANIL K, COOPER, ALAN, BRUCE, GANGULY, ASHIT, KUMAR, GIRIJAVALLABHAN, VIYYOOR, MOOPIL, GUZIK, HENRY, SAKSENA, ANIL, KUMAR
Applicants: SCHERING CORP, SCHERING CORPORATION
Application year: 1987
Main classification: C07D405
IPC codes: A61K31, A61P29, A61P31, A61P37, A61P43, C07D233, C07D249, C07D405, C07D521