A PROCESS FOR SUPPLY OF BIOLOGICALLY ACTIVE MATERIALS TO BASE MATERIALS

Abstract: A process for supply of low concentrations of biological­ ly active materials to base materials such as a processed foodstuff or an animal food, in which the biologiocally active material is slurried in an inert carrier, where it is insoluble, to form a homogeneous suspension, after which the suspen­ sion is applied to the base material by means of a device converting the suspension to an easily applicable form, the suspension coating the surface of the base material in an even layer. The suspension is maintained homogeneous by stirring and at an even temperature before being applied to the base material at such a rate that an even and predeter­ mined concentration of the biologically active material is obtained per weight unit distributed on the surface of the base material.

Description: This invention relates to a process for supply of biologically active materials to base material, such as processed foodstuff and feed, and especially to such a process where low concentrations, preferably of the order of parts per million of biologically active materials are supplied to the base material. It is known that ever more foodstuffs and feed are subjected to processes, by the purpose of which is to improve the nutrient properties, taste, structure, handling ability etc. of the foodstuff and feed. Most of these processes utilize heat, pressure, grinding and other physical working often in combination with supply of steam or another form of heat. Examples of such processes are rolling, steam boiling, extrusion, pelletizing etc. For the purposes of this description the expression "base material" concerns foodstuffs and feed that have been subjected to the processes described above It is also known that certain sensitive nutrients such as vitamins, enzymes and recently also living bacteria which are intended to be supplied to a base material, do not stand certain steps in the processes described above but are destroyed or, in any case, lose their so-called biopotency completely or partly. One way of trying to avoid a decrease of the biopotency of the biologically active material or denaturation of the material is for instance described in Swedish patent application 7902104-4, where bacteria are encapsulated in for example fats or the like. The material thus encapsulated, for example admixed to animal food, stands for instance pelletizing better than materials not being encapsulated. One way of evading the problem of denaturation should be to supply biologically active material to a processed feed or foodstuff, i.e. to the base material. However, such a process involves considerable technical difficulties. For instance, one difficulty may be that the biologically active material, the amount of which is small relative to the base material, cannot be evenly distributed therein. Another problem is that the even admixture is not very likely to remain homogeneous, even if one should be successful in mixing a small amount of biological material homogeneously with a large amount of base material, but with all probability the material will be stratified in a subsequent handling. Still a problem in connection with an admixture of biologically active material in foodstuffs or animal food is the known phenomenon friction between the particles to be homogenized. A slow and careful homogenization usually results in a bad homogenity while an efficient homogenization destroys biologically active material completely or partly. One method of reducing the risks of a similar reduction of the bioactivity is using for example encapsulated material, as mentioned above. However, the homogenity problem cannot be solved by this method, particularly not as the density and the particle size of the materials to be mixed differ from each other. A way of solving the problem with a homogeneous and constant admixture-of material in for example seed is described in Swedish application 7904283-4, where a finely divided material to be supplied to seed or grain for instance is mixed with e.g. oil or fat to a paste which is sorbed by the seed. However, the method implies synergistic components of the mixture with respect of sorption of the seed; moreover, relatively large amounts of additives are required for a homogeneous admixture. As certain biologically active materials are active also in amounts of the order of parts per million, the method is not useable for supply of such materials. Another disadvantage of the method is that the carrier used together with finely divided material is supplied in small amounts which, therefore, cannot possibly protect biologically active materials from physical and chemical effects or contribute to a homogeneous admixture, especially as small amounts of active material are to be bonded to the seed. Certain biologically active materials are sensitive to moisture. Examples of this are certain freeze-dried bacteria concentrates. As one has recently started to use living bacteria for admixture in for example dry feed or dry foodstuff an increased demand is made on the stability of these active materials against for instance moisture. One way of increasing the resistance to moisture of the active material, e.g. the moisture resistance of freeze-dried bacteria, is for instance described in CH A 525 957, according to which a freeze-dried bacteria mass is mixed with oils or fats to a paste which will then have a certain protection. However, it is known that a homogeneous admixture of such a paste in for instance feed or foodstuff implies a relatively large amount of bacteria paste, probably 5-10% or more. Otherwise the homogenization must be so effective that the survival of the bacteria is risked as well as the structural properties of the material, which can be changed. It is apparent from the above-mentioned description of prior art that none of the methods described therein is suitable to utilize when it is desired to add small amounts of biologically active materials to base materials, i.e. processed feed or foodstuffs, in such a way that the biologically active material is distributed homogeneously, on one hand, and the active material, on the other hand, is protected at the same time against mechanical and other physical strains such as mixture, transport and storage. Accordingly this invention relates to a process for supply of low concentrations of biologically active materials to base material such as processed foodstuff and feed, and the process has been given the characteristic features apparent from claim 1. In accordance with a preferred embodiment of the invention the biologically active material is present in a solid and finely divided form and the carrier is a liquid, which has a polarity opposite to that of the solid biologically active material (i.e. a system having hydrophilic-hydrophobic properties). This means that if the biological material is hydrophilic, i.e. polar, the carrier must be hydrophobic, or nonpolar. The reason for this is that many materials such as biologically active materials, for instance enzymes, freezedried bacteria, etc. are easily denaturated in a hydrophilic environment, for example when these are admixed in water; this is especially the case when water solutions of such materials are dried e.g. at room temperature. Such a negative side-effect can be avoided by the above-mentioned opposite polar-nonpolar relation between the solid biological material and the liquid carrier. In such a case the carrier gives several other positive qualities in connection with its use. It is possible to admix homogeneously also very small amounts of biologically active material in a base material, on one hand, and, on the other hand, the carrier serves as a friction reducing agent in admixture and transport. Moreover, it is avoided that the properties of the active material are influenced by the carrier. Besides, the carrier serves as an adhesive material contributing to the active material being retained on the surface of the processed product. If the carrier is an oil it functions as a tacky binder. If, for instance, it consists of water or some other polar substance a small amount of for example sugar or the like is dissolved from the feed or foodstuft which, then, adheres to the added material on the surface of the particles. The very supply technique is known per se and is for instance utilized in coating of a processed material with e.g. an aromatic or dye which, however, must be quite dissolved in the liquid carrier according to usual technique. However, this demand is not only quite unnecessary according to the invention but even a clear disadvantage in biologically active materials, the properties of which are influenced negatively by the liquid carrier if the material should be dissolved therein. The invention will now be illustrated with the following on-limiting example. 10 g of a biological material, enzyme or freeze-dried bacteria, the properties of which are hydrophilic (polar), are mixed homogeneously with 1 000 ml of a hydrophobic liquid, for instance oil, to form a suspension. This suspension is added to 1 000 kg of feed or foodstuff pelletized, extruded or treated in another way, by means of a membrane pump, the suspension being pumped through an atomizing nozzle. The suspension which is finely divided in the nozzle to very small particles is led to the processed material e.g. in a chamber or a transport pipe which is provided with a device mixing and holding the material until this has been mixed homogeneously with the finely divided suspension. In accordance with the above example 10 ppm of a biologically active material can thus be supplied to the base material, i.e. the processed foodstuff or feed, with the following unexpected results; the biologically active material is mixed homogeneously with the base material and is evenly distributed on the latter's surface, negative friction effects in dosage and admixture are eliminated, the added biological material is retained on the surface of the base material through the action of the carrier. It is apparent from this example that so small amounts as of the order of parts per million of a biologically active material can be added efficiently and leniently to a processed animal food or foodstuff without the properties or the biologicl activity of the biologically active material beinginjuriously influenced. Thanks to these circumstances the invention will have a great economical and technical importance in modern production of animal food and/or foodstuffs.

Claims: 1. A process for supplying low concentrations, preferably in the order of parts per million, of biologically active materials to a base material, such as processed foodstuff or animal feedstuff, characterized in that the biologically active material present in a solid and finely divided form is slurried in an inert carrier in the form of a liquid having opposite polarity relative to the biologically active material to be suspended, in which the biologically active material is insoluble, to the formation of a homogeneous suspension, the carrier forming a protection for the admixed material against physical and chemical influence detrimental to the biologically active material, that the suspension is applied to the base material which is intended to be combined with the biologically active material admixed in the suspension, the suspension being provided in an easily applicable form, whereby the suspension is coating the surface of the base material in an even layer, that the suspension is maintained homogeneous by means of stirring and at even temperature, and that the suspension is supplied to the base material to be coated at such a rate that an even and predetermined concentration of the suspended biologically active material is obtained per weight unit and is distributed on the surface of the base material. 2. Process according to claim 1, characterized in that the biologically active material is selected from the group of enzymes, vitamins, hormones, living bacteria or parts thereof, or mixtures of these. 3. Process according to claim 1, characterized in that the carrier is one preferred by humans and animals.

Inventors: ALEXANDERSSON, LARS, KVANTA, ENDRE, PEDERSEN, ANDERS
Applicants: AB MEDIPHARM
Application year: 1987
Main classification: C12N1
IPC codes: A23K1, A23L1