QUINOLINECARBOXYLIC ACID DERIVATIVES

Abstract: Quinolinecarboxylic acid derivatives represented by the following general formula (I) and physiologically-acceptable salts thereof. wherein R¹ is hydrogen, alkyl, or substituted or unsubstituted phenyl; R² is hydrogen, alkyl, aikoxy, hydroxy, halogen, nitro or substituted or unsubstituted amino; R³ is hydrogen or optionally-substituted alkyl; R⁴ and R⁵ are same or different and are alkyl or hydroxyalkyl or R⁴ and R⁵ may be combined and may form a cyclic amino together with the adjacent nit­ rogen atom and said cyclic amino may also be substituted and may further contain nitrogen, oxygen or sulfur as hetero-­ atoms therein; and X is halogen. have been found to be very effective antibacterial therapeutic agents for systemic and local infectious diseases caused by P. aeruginosa and Gram­ positive and negative bacteria.

Description: The present invention relates to novel quinolinecarboxylic acid derivatives exhibiting antibacterial action and useful as therapeutic agents for various infectious diseases. More particularly, it relates to quinolinecarboxylic acid derivatives represented by the following general formula (I) and physiologically-acceptable salts thereof. wherein R¹ is hydrogen, alkyl having 1 to 6 carbon atoms or phenyl unsubstituted or substituted with alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, trifluoromethyl or nitro; R² is hydrogen, alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, nitro or amino unsubstituted or substituted with acyl having 2 to 3 carbon atoms; R³ is hydrogen or alkyl having 1 to 6 carbon atoms unsubstituted or substituted with hydroxy, acyloxy having 2 to 6 carbon atoms or alkoxy having 1 to 4 carbon atoms; R⁴ and R⁵ are same or different and are alkyl having 1 to 4 carbon atoms or hydroxyalkyl having 1 to 4 carbon atoms or R⁴ and R⁵ may be combined and may form a 4- to 8-membered cyclic amino together with the adjacent nitrogen atom, selected from the group consisting of azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino and pyrazolino, and said cyclic amino may also be substituted with 1 to 3 substituents selected from the group consisting of alkyl having 1 to 6 carbon atoms, alkenyl having 2 to 6 carbon atoms, alkynyl having 2 to 6 carbon atoms, aryl having 6 to 12 carbon atoms, aralkyl having 7 to 12 carbon atoms, hydroxyalkyl having 1 to 4 carbon atoms, alkoxyalkyl having 2 to 6 carbon atoms (said alkoxyalkyl optionally substituted by hydroxyl), aminoalkyl having 1 to 4 carbon atoms, cyanoalkyl having 2 to 4 carbon atoms, carboethoxymethyl, acylalkyl having 3 to 8 carbon atoms, acyl having 1 to 6 carbon atoms, hydroxyl, oxo, amino, alkylamino having 1 to 4 carbon atoms or dialkylamino having 1 to 4 carbon atoms and X is halogen. Furthermore, the present invention relates to a method of manufacturing quinolinecarboxylic acid derivatives represented by the general formula (I) and physiologically salts thereof and a pharmaceutical composition useful for treating bacterial or fungal infections in humans and animals comprising a therapeutically effective amount of a compound having the general formula (I). As to synthetic antibacterials as remedies for infectious diseases caused by Gram negative bacteria, nalidixic acid, piromidic acid, pipemidic acid, enoxacin (AT-2266), ofloxacin (DL-8280) etc have been widely used but they are not satisfactory as remedies for infectious diseases caused by Gram positive bacteria and those by Pseudomonas aeruginosa (chronic) which have been increasing in recent days and are hard to be cured. In solving such a problem, various compounds have been synthesized and many patent applications have been filed. The present inventors have synthesized various compounds too, found quinolinecarboxylic acid having excellent antibacterial action, and filed patent applications already (cf. Japanese Laid Open Applications 57/136,588 and 58/103,393). In those applications, thiazoloquinolinecarboxylic acids are mainly disclosed and, as a compound similar to the present invention compounds, 7-chloro-6-fluoro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid is disclosed as a starting material. Also there is a disclosure in U. S. Patent No.4,550,104 as to thiazoloquinolinecarboxylic acid derivatives. (Problems to be solved by the Invention) There is a certain limit as to the effect in those antibacterials and, as to the safety, there is no satisfactory result at all times. In overcoming those difficulties, the present inventors continued the studies, found a series of compounds which exhibit far better pharmacological effect than the prior art ones with less toxicity, and accomplished the present invention. Accordingly, an object of the present invention is to develop novel pharmaceuticals which are markedly better than the conventional synthetic antibacterials as already mentioned. Compounds of the present invention are novel substances which have not been described in the prior art literatures yet. The characteristic features in chemical structure are in the following two points: (1) A ring formed between the nitrogen and sulfur atom of the 2-mercaptoquinoline skeleton is a thiazetidine ring; and (2) 6-position of the quinoline skeleton and 7-position of it are substituted with halogen and with amine, respectively. Preferred alkyl represented by R¹ in the general formula (I) is straight or branched lower alkyl having 1 to 6 carbon atoms and, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, n-hexyl, isohexyl, etc. Examples of substituents of phenyl represented by R¹ are alkyl, alkoxy, hydroxy, halogen, trifluoromethyl, nitro, etc. Examples of the alkyls as such substituents are those exemplified as hereinabove. As to alkoxy, straight or branched lower alkoxy with 1 to 4 carbon atoms is preferred and is, for example, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, tert-butoxy, and the like. As to halogen, chlorine, bromine, iodine and fluorine may be listed and, among them, fluorine is especially preferred. Examples of the alkyl, alkoxy and halogen represented by R² are those exemplified as subtituents for phenyl hereinabove. Examples of the substituted amino are acylamino (e.g. acetylamino and propionylamino). Examples of alkyl represented by R³, those exemplified by R¹ may be listed. Such an alkyl may be substituted with hydroxy, alkoxy, acyloxy, etc. As to a substituent for the alkyl, the above-exemplified ones may be used. Examples of acyloxy are acetyloxy, propionyloxy, butyryloxy, pivaroyloxy, and other ones with 2 to 6 carbon atoms. As to halogen represented by X, fluorine or chlorine may be listed. Alkyl represented by R⁴ and R⁵ is preferably straight or branched lower alkyl having 1 to 4 carbon atoms, e.g. methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, etc. Preferred hydroxylalkyl represented by R⁴ and R⁵ is that with 1 to 4 carbon atoms and is, e.g., 2-hydroxyethyl, 3-hydroxypropyl, 2-hydroxypropyl, etc. Examples of cyclic amino represented by it is, for example, 4 to 8 membered one and it may further contain nitrogen, oxygen or sulfur atoms therein. Examples of such cyclic amino is azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino, pyrazolino, etc. Such a cyclic amino may also have one to three substituent(s) which may be same or different. Examples of such substituents are alkyl, alkenyl, alkynyl, aryl, aralkyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, cyanoalkyl, carboalkoxyalkyl, acylalkyl, acyl, hydroxy, oxo, amino, alkylamino, dialkylamino etc. The alkyl of such a substituent may include those exemplified for R¹ hereinabove. As to the alkenyl, straight or branched lower alkenyl having 2 to 6 carbon atoms are preferred and is, for example, vinyl, allyl, isopropenyl, 2-methallyl, 2-butenyl, 3-butenyl, etc. Examples of alkynyl are straight or branched lower alkynyl with 2 to 6 carbon atoms and are, for example, ethynyl, 1-propynyl, and 2-propynyl. Examples of the aryl are phenyl, alpha-naphthyl, beta-naphthyl, biphenyl, etc., and among them, phenyl is especially preferred. Examples of aralkyl are preferably those with 7 to 12 carbon atoms and are, for example, benzyl, phenethyl, phenylpropyl, naphthylmethyl, and the like. As to hydroxyalkyl, that with 1 to 4 carbons is preferred and is, for example, hydroxymethyl, 2-hydroxyethyl, 3-hydroxypropyl, 2-hydroxypropyl, etc. As to alkoxyalkyl, that with 2 to 6 carbon atoms is preferred and is, for example, methoxyethyl, methoxypropyl, ethoxymethyl, and ethoxyethyl. Such an alkoxyalkyl may be further substituted with hydroxyl. As to aminoalkyl, that with 1 to 4 carbons is preferred and is, for example, aminomethyl, 2-aminoethyl, 3-aminopropyl, 4-aminobutyl, etc. As to cyanoalkyl, that with 2 to 4 carbons is preferred and is, for example, cyanomethyl, 2-cyanoethyl, and 3-cyanopropyl. As to acylalkyl, that with 3 to 8 carbons is preferred and is, for example, benzoyl or the acyl (as exemplified in the acyloxy for R³) to which alkylene of 1 to 4 carbons is combined. Such an acylalkyl may be further substituted with carboxy, carbomethoxy, carboethoxy, etc. As to acyl, that with 1 to 6 carbons is preferred and is, for example, formyl, acetyl, propionyl, n-butyryl, isobutyryl, valeryl, isovaleryl, pivaloyl, n-hexanoyl, trifluoroacetyl, etc. As to alkylamino, that with 1 to 4 carbon atoms is preferred and is, for example, methylamino, ethylamino, n-propylamino, isopropylamino, n-butylamino, etc. Examples of dialkylamino are dimethylamino, diethylamino, di-(n-propyl)-amino, etc. When those substituents have benzene rings, the rings may be further substituted with alkoxy or amino which is exemplified as hereinabove. Examples of the salts of the compound (I) of the present invention are salts with mineral acids such as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, etc., salts with organic acids such as formic acid, acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, camphorsulfonic acid, etc., and salts with alkali metal or alkali earth metal such as sodium, potassium, calcium, etc. As to representative examples of the present invention, the following compounds, for example, may be listed in addition to the compounds given in the Examples. Ethyl 6-fluoro-1-(4-fluorophenyl)-7-(4-methyl-1-1piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 6-fluoro-1-(4-fluorophenyl)-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 6-fluoro-1-(4-fluorophenyl)-7-(3-methyl-1-piperazinyl)-4-oxo- 4H -[1,3]thiaeto[3,2-a]quinoline-3-carboxylate; ethyl 6-fluoro-1-(4-fluoro-phenyl)-7-(3,4-dimethyl-1-piperzinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 1-(4-chlorophenyl) -6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 1-(4-chlorophenyl)-6-fluoro-4-oxo-7-(1-piperazinyl)- 4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 1-(4-chlorophenyl)-6-fluoro-7-(3-methyl-1-piperazinyl)-4-oxok-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 1-(4-chlorophenyl)-6-fluoro-7-(3,4-dimethyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboylate; and 6-fluoro-8-methoxy-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid (m.p. 193°C (decompn.); ethyl ester, m.p. 190 o C (decompn.)). The present invention compound may, for example, be manufactured by the following methods: Method A In the formulae, R¹, R², R⁴, R⁵ and X are the same as those already defined; Y and Z are same or different halogens; and R³ is alkyl. Method B In the formulae, R¹, R², R³, R⁴, R⁵, X, Y and Z are the same as those in the Method A. Method C In the formulae, X, R¹, R², R³, R⁴, and R⁵ are the same as those in the Method A. Method D In the formulae, X, R¹, R², R⁴ and R⁵ are the same as those hereinabove. It will be apparent from the above descriptions that the present invention compounds are manufactured, when roughly classified, by two routes. One is to manufacture thiazetidine ring using quinolinecarboxylic acid substituted with amino group at 7-position as a starting material (Methods A and B) and another is to produce thiazetidine ring followed by introducing amino group at 7-position (Methods C and D). As hereunder, each method will be illustrated in some more detail. Method A: (II) and dihalide (e.g. methylene iodide, ethylidene bromide, benzylidene bromide, etc.) are made to react usually at 0 to 120°C in the presence of an acid removing agent (e.g. sodium carbonate, potassium carbonate, triethylamine, etc.) in a solvent which is inactive to the reaction whereupon a cyclization is resulted giving (Ia). As to the solvent, nonprotonic solvent such as N,N-dinethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, sulphorane, etc. is preferred. The amounts of dihalide and acid removing agent are preferably not less than equimole and, more preferably 1.1 to 2.5 moles, to 1 mole of (II). In order to accelerate the reaction, the reaction may be conducted by addition of catalytic amount (0.01 to 0.1 molar equivalent) of sodium iodide or potassium iodide. Method B: (II) and the halide (ZCH₂R¹) are generally made to react at 0 to 80°C using the same solvent and acid removing agent as in the Method A to manufacture (IV). Then the (IV) is halogenated with a halogenating agent (e.g. N-bromosuccinimide, N-chlorosuccinimide, etc.) in an inert solvent (e.g. chloroform, dichloromethane, carbon tetrachloride and other halogenated hydrocarbon type solvent) to give (V). Then (V) is cyclized generally at 0 to 80°C by the use of the same solvent and acid removing agent as in the Method A to afford (Ia). Method C: (VI) is reacted with an amine to give (Ia). In this reaction, amine is made to react in a solvent which is inactive to the reaction (e.g. N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, sulforane, acetonitrile, and other nonprotonic solvent) usually at 0 to 80°C (e.g. 40 to 60°C). The amount of the amine is 1.5 to 2.5 moles to one mole of (VI). Method D: (VI) is hydrolyzed using an acid (e.g. concentrated sulfuric acid, fuming sulfuric acid or a mixture thereof) to give (VII). This reaction is conducted using an excess (e.g. 1 to 30 times excess by weight and, more preferably, 5 to 10 times excess by weight) of acid as a solvent usually at 0 to 60°C. This hydrolysis reaction may also be conducted in 20 to 40 times excess by weight (preferably 5 to 10 times excess by weight) of 1-5% potassium hydroxide or sodium hydroxide in aqueous alcohol (methanol, ethanol, propanol, butanol etc.) generally at room temperature to 60° C. Then (VII) is made to react with amine in the same solvent as used in Method C to give (Ib). The reaction is usually conducted at 0 to 60°C and, more preferably, 0°C to room temperature. There are some other uethods and one of them is that, starting from a compound of general formula (VIII), the product can be manufactured by the following route. In the formulae, definitions for R¹, R², R³, R⁴, R⁵ and X are the same as those in the Method A. Thus, (VIII) and a dihalide are made to react in the presence of an acid removing agent (e.g. potassium carbonate) in an inert solvent (e.g. N,N-dimethylformamide). Then (IX) is subjected to a ring closure to manufacture (Ia). This ring closure reaction can be done by a known method per se such as, for example, a method by heating and a method using acidic substance such as phosphorus oxychloride, phosphorus pentachloride, phosphorus trichloride, thionyl chloride, fuming sulfuric acid, concentrated sulfuric acid, polyphosphoric acid, polyphosphate, etc. When an acidic substance is applied, its amount is 1 mole to large excess (more preferably 20 to 30 moles) of acidic substance to 1 mole of (IX) and the reaction is usually conducted at 0 to 100°C (more preferably at 0 to 60°C). It is also possible that a thiazetidine ring is formed starting from 3,4-difluoro substance and then condensed with an amine by the same way as in the Method C to give (Ia). When R² is hydrogen, (X) is produced as a side product and, after removing it from the reaction mixture, the residue is made to react with an amine. Or the separation is carried out after the condensation with an amine. When diamine such as piperazine is used as a reactant in the above manufacturing methods, one of the amine groups is protected, if necessary, by a known method. The amine is made to react with (VI) and then the protective groups are detached to afford the desired compound (N-nonsubstituted compound). It is also possible that substituent(s) is (are) introduced to the nitrogen atom to the N-nonsubstituted compound by a known method per se to manufacture N-substituted diamino compound. 8-Substituted derivative can also be manufactured by introduction of desired substituent(s) to the compound obtained hereinabove (where R² is hydrogen) by a known method per se. When the compound manufactured by the above methods is ester (i.e. R³ is alkyl), it is hydrolyzed, if desired, to give a carboxylic acid (i.e. R³ is hydrogen). This hydrolysing reaction is conducted by the use of a large excess of acid (e.g. sulfuric acid, fuming sulfuric acid, hydrochloric acid, hydrobromic acid, hydrobromic acid/acetic acid, chlorosulfonic acid, polyphosphoric acid, etc.) and, more preferably 10 to 20 times excess of acid as a solvent, at room temperature to 110°C. Alternatively, the hydrolysis may also be conducted by stirring, at room temperature to 60°C, in a 1 to 5% aqueous alcohol (e.g., methanol, ethanol, propanol and butanol; among them, tert-butanol is preferred) of 20 to 30 times excess (preferably 5 to 10 times excess) of potassium hydroxide or sodium hydroxide. Another manner will be that the ester is heated with stirring at 60-150°C, preferably at 100 to 110°C, in 10 to 100 times excess of alcohol corresponding to desired ester in the presence of catalytic amount of concentrated sulfuric acid whereupon desired ester can be afforded. In the case of carboxylic acid (R³ is hydrogen), it is, if desired, esterified to give an ester (i.e. R³ is alkyl). In this esterification, an esterification method known per se such as, for example, the use of thionyl chloride and alcohol; alcohol and condensing agent (e.g. dicyclohexylcarbodiimide); or alkyl halide and alcoholate. In the case of carboxylic acid, it may be used in a form of pharmacologically-acceptable salt (e.g. sodium or potassium salts) by a known method per se. Some of the starting compounds (II) and (VIII) are novel and such novel compounds are manufactured by a known method (e.g. a method disclosed in Japanese Laid Open Application 57/136588) or by the same method as the Reference Examples given later. Novel starting compounds (VI) are given later in the Reference Examples and they are manufactured by the same or similar manner as in the above Method A or Method B. The amine is a known substance or can be manufactured by the similar manner in the known methods. The desired compound (I) prepared as such can be separated and purified by various means known per se such as, for example, concentration, conversion of liquid properties, transfer to another solvent, extraction with a solvent, crystallization, recrystallization, fractional distillation, chromatography, and the like. When the compounds of the present invention are administered as drugs, they are given to animal including human being as they are or as a pharmaceutical composition containing 0.1 to 99.5%, more preferably 0.5 to 90%, of the present invention compound in nontoxic and inert pharmaceutically-acceptable carrier. As to the carrier, one or more solid, semisolid or liquid diluent, filler, or other auxiliary agents for pharmaceutical preparations can be used. It is desired that the pharmaceutical compositions are given in a unit dose form. The pharmaceutical composition of the present invention can be administered from mouth, tissue, local place (via skin), or rectum. It is needless to say that the administration is done by a form suitable to each administration method. Oral administration is especially preferred. It is desired that the dose as a remedy for infectious diseases is adjusted taking the state of the patients (e.g. age, body weight, etc.), administration route, type and degree of diseases, and the like into consideration. Usually, the dose is 50 to 1000 mg/day/human being (more preferably, 100 to 330 mg/day/human being) calculated as an amount of the effective ingredient of the present invention. In some cases, less dose will do while, in some other case, more dose may be required. It is desired that the dose is divided into two or three times a day. (Example) As hereunder, several reference examples for the manufacture of the present invention, example, and test examples of the present invention compounds are given for further illustration of the present invention. Reference Example 1. Ethyl 6,7-difluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. (1) 3,4-Difluoroaniline (600 g) and 1410 g of triethylamine were mixed and, with ice cooling and stirring, 389 g of carbon disulfide was dropped in during 2.5 hours. The mixture was stirred at the same temperature for 2 hours more whereupon crystals gradually appeared. The mixture was gradually warmed up to the room temperature, stirred for 2 hours, and kept in a refrigerator for two nights. Chloroform (2 liters) was added to the reaction mixture in which crystals were solidified, the mixture was stirred to make it suspended, and ethyl chloroformate was dropped in at 10°C (inner temperature) during 2 hours. The mixture was stirred for 3.5 hours more at room temperature. After the reaction, the reaction solution was poured over into ice water, the mixture was made weakly acidic with concentrated hydrochloric acid, and the chloroforn layer was collected. This was washed with water, dried and concentrated and the residue was purified by a column chromatography (n-hexane/silica gel) to give 558.6 g of 3,4-difluorophenyl isothiocyanate in colorless oil. (2) Ethyl malonate (200.3 g) was dropped into a stirring mixture of 2850 ml of dioxane and finely-powdered potassiun hydroxide (70.2 g) during 3 hours. After the dropping was completed, 186.2 g of oil obtained in (1) was dropped in at room temperature with stirring and then the mixture was stirred for 18 hours. Methoxymethyl chloride (100.2 g) was gradually dropped in with ice cooling and stirring, then stirred at room temperature for 3 hours, poured over into ice water, extracted with ethyl acetate, and the extract was washed with water and dried. The resulting residue was purified by a silica gel column chromatography (n-hexane/ethyl acetate (2:1) was used as an eluting solution) to give 383.8 g of oily diethyl 1-(3,4-difluorophenylamino)-1-(methoxymethylthio)-methylenemalonate. (3) The oily substance (85.5 g) obtained in the above (2) was dissolved in 250 g of diphenyl ether and the solution was heated with stirring at 240°C for 5 to 10 minutes. This was cooled to 80°C, poured over into 1 liter of n-hexane, and allowed to stand overnight in a cool place. Crystals separated out therefrom were collected by filtration and washed with n-hexane, to give 195 g of ethyl 6,7-difluoro-4-hydroxy-2-methoxymethylthioquinoline-3-carboxylate, pale yellow crystals, m.p. 126-129°C. (4) Concentrated hydrochloric acid (600 ml) was dropped into a suspension of 195g of the crystals obtained in (3) in 1 liter of ethanol at room temperature with stirring. After the dropping was completed, the mixture was stirred for 2 hours, ice water was added thereto, the crystals separated out therefrom were collected by filtration, washed with water and air-dried to give 166.6 g of ethyl 6,7-difluoro-4-hydroxy-2-mercaptoquinoline-3-carboxylate, yellow crystals, m.p. 201-203°C (decomposition). (5) Ethylidene bromide (110.0 g), 77.4 g of potassium carbonate, 4.6 g of potassium iodide and 540 ml of N,N-dimethylformamide were placed in a flask and heated with stirring at 105-110°C. A solution of 80 g of crystals obtained in (4) in 1400 ml of N,N-dimethylformamide was dropped into the above solution. After the dropping was completed, the mixture was stirred at the same temperature for 2.5 hours. After the reaction was completed, the mixture was concentrated in vacuo. The concentrated solution was poured over into ice water and crystals separated out were collected with filtration followed by washing with water and drying with air to give 61.4 g of ethyl 6,7-difluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate as crude crystals. This was recrystallised from a 10:1 mixture of chloroform and methanol to give 41.2 g of colorless crystals. Melting point: 200-202°C. Elementary analysis calculated for C₁₄H₁₁F₂NO₃S. Calcd (%): C 54.02, H 3.56, N 4.50 Found (%): C 54.54, H 3.42, N 4.29 Reference Example 2. (1) 2-Fluoro-6-methoxyaniline (7.80 g) and 17.23 g of di-(2-bromoethyl)amine hydrobromide were dissolved in 5 ml of water and, by heating at about 110°C on a bath, 10 ml of 30% potassium hydroxide solution was added thereto (each about 3.5 ml once an hour). Heating was further continued (7 hours in total). After cooled, the reaction solution was made alkaline with aqueous solution of sodium hydroxide to salt out and extracted with chloroform twice. The extract was washed with saturated sodium chloride solution, dried over sodium sulfate, the solvent was evaporated therefrom, and the residue was purified by a column chromatography to give 8.95 g of oily 1-(2-fluoro-6-methoxyphenyl)piperazine in 34.94 g yield or 94%. (2) The compound (34.89 g) obtained by the same manner as in the above (1) was dissolved in 50 g of formic acid and 135 g of 37% formaline and the solution was heated to reflux for 75 minutes at the bath temperature of 110°C. The reaction solution was concentrated in vacuo and dissolved in diluted hydrochloric acid. Insoluble matters were removed, the residue was washed with ethyl acetate, made alkaline with aqueous solution of sodium hydroxide, salted out, and extracted with chloroform. The extract was dried over sodium sulfate and the solvent was evaporated therefrom. The residue was dissolved in ether, hydrochloric acid/ethanol was added thereto, and the hydrochloride was separated out therefrom. The crystals were washed with ether, dried, dissolved in water, sodium hydroxide solution was added, and extracted with ether. The extract was washed with saturated sodium chloride solution, dried over sodium sulfate, and the solvent was evaporated therefrom to give 26.18 g of oily 1-(2-fluoro-6-methoxyphenyl)-4-methyl-piperazine, yield 70%. (3) The compound (27.56 g) obtained by the same manner as in the above (2) and 42.14 g of silver sulfate were dissolved in 600 ml of concentrated sulfuric acid, a solution of 21.60g of bromine in 1200 ml of concentrated sulfuric acid was dropped therein during aoout 30 minutes with ice-cooling, and the stirring was continued for another 90 minutes. The reaction solution was poured over into ice, the mixture was made alkaline with sodium hydroxide with ice cooling, and extracted with chloroform twice. The extract was washed with saturated sodium chloride solution and dried over sodium sulfate. The solvent was evaporated therefrom and purified by a column chromatography to give 14.12 g of 1-(3-bromo-2-fluoro-6-methoxyphenyl)-4-methylpiperazine. Yield 38%. (4) The compound (13.42 g) obtained by the same manner as in the above (3) was dissolved in 500 ml of concentrated sulfuric acid and a solution of 4.70 g of potassium nitrate in 70 ml of concentrated sulfuric acid was dropped in during 20 minutes with ice-cooling (temperature being 4 to 6°C). This was stirred for 30 minutes more, then poured over into ice water, the mixture was made weakly alkaline with sodium bicarbonate, and extracted with ethyl acetate. The extract was washed with saturated sodium chloride solution and dried over sodium sulfate. The solvent was evaported therefrom and purified with a column chromatography to give 3.29g of 1-(3-bromo-2-fluoro-5-nitro-6-methoxyphenyl)-4-methylpiperazine. Yield 21%. (5) The compound (2.778 g) obtained by the same manner as in the above (4) was dissolved in 60 ml of concentrated hydrochloric acid and a solution of 7.38 g of stannous chloride dihydrate in 80 ml of concentrated hydrochloric acid was dropped in with ice-cooling. After completion of the dropping, the mixture was stirred for 30 minutes more, poured over into ice, neutralized with diluted sodium hydroxide, and extracted with chloroform. The extract was washed with saturated sodium bicarbonate solution, dried over sodium sulfate, and the solvent was evaporated therefrom in vacuo. As a residue was obtained 2.432 g of 1-(5-amino-3-bromo-2-fluoro-6-methoxyphenyl)-4-methylpiperazine. Yield 96%. (6) The compound (2.33 g) obtained by the same manner as in the above (5) was dissolved in 150 ml of ethanol, then 0.30 g of sodium hydroxide and 200mg of 5% palladium-carbon were added, and the catalytic reduction was conducted at room temperature and ordinary pressure. The reaction solution was filtered and the filtrate was concentrated in vacuo. This was extracted with chloroform, the extract was washed with saturated sodium bicarbonate solution, dried over sodium sulfate and the solvent was evaporated therefrom to give 1.68 g of 1-(5-amino-2-fluoro-6-methoxyphenyl)-4-methylpiperazine. Example 1. (1) 6-Fuoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. To 50.0 g of cooled and stirred fuming sulfuric acid was little by little and gradually added 4.73 g of crystals of ethyl 6,7-difluoro-1-methyl-4-oxo-4H-[1,3]-thiaceto[3,2-a]quinoline-3-carboxylate manufactured by the same manner as in (5) of the Reference Example 1 and dissolved in. The resulting solution was stirred at 60 o C for 30 minutes. The reaction solution was poured over into ice, the milky crystals separated out were centrifuged, washed with water, and air-dried to give 4.02 g of powdery crystals of 6,7-difluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 269-272°C (decompn). (2) A mixture of 14.1 g of crystals obtained in the above (1) and 300 ml of N,N-dimethylformamide was cooled in ice water and, with stirring, 10.6 g of N-methylpiperazine was gradually dropped in. After that, the mixture was stirred for 22 hours at room temperature. After the reaction, N,N-dimethylformamide was evaporated in vacuo. To the residue was added acetone and the crystals separated out were collected by filtration followed by washing with acetone, chloroform and ether to give 12.63 g of crystals of desired compound, m.p. 262°C (decompn.). Elementary analysis calculated for C₁₃H₁₈FN₃O₃S: Calcd (%): C 56.18, H 4.99, N 11.56 Found (%): C 56.17, H 5.18, N 11.48 Hydochloride melts at 288-290°C (decompn); sulfate melts at 252°C; methanesulfonate melts at 251-253°C (decompn); toluenesulfonate melts at 265-268°C (decompn); 2-naphthalenesulfonate melts at 207-210°C (decompn); 1-naphthalenesulfonate melts at 223-225°C (decompn); ethanesulfonate melts at 273-275°C (decompn); d-10-camphorsulfonate melts at 257-259°C (decompn); benzensulfonate melts at 246- 248°C (decompn); maleate melts at 238-240°C (decompn,); and trlphosphate melts at 201-203°C (decompn,). Methanolic solution of sodium methylate was prepared from 0.126 g of sodium and 30 ml of anhydrous methanol and 2.0 g of the carboxylic acid as obtained above was added thereto followed by stirring for about 2 hours. The crystals separated out were collected by filtration after evaporation of methanol. The crystals were washed with methanol, acetone and ether and dried to give 2.1 g of sodium salt, m.p. 306-309°C (decompn.) Example 2. Ethyl 7-(4-acetyl-1-piperazinyl)-6-chloro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. Into 50 ml of N,N-dimethylformamide were placed 2.35 g of methylene iodide and 2.5 g of potassium carbonate and a solution of 3.0 g of ethyl 7-(4-acetyl-1-piperazinyl)-6-chloro-4-hydroxy-2-mercaptoquinoline-3-carboxylate in 30 ml of N,N-dimethylformamide was gradually dropped in with stirring. After that, the mixture was stirred at room temperature for 1 hour, concentrated in vacuo, water was added to the residue, and insoluble crystals were collected by filtration. They were washed with water, dried, and recrystallized from a mixed solvent of ethanol and chloroform to give 2.49 g of colorless powdery crystals,m.p. 266-268°C (decompn). Elementary analysis calculated for C₁₉H₂₀ClN₃O₄S. 1/2 H₂O: Calcd (%): C 52.96. H 4.91, N 9.75 Found (%): C 53.16, H 4.69, N 9.69 Example 3. 6-Chloro-1-methyl-7-(1-piperazinyl)-4-oxo-4H-[1,3]-thiaceto[3,2-a]quinoline-3-carboxylic acid. A mixture of 610 mg of ethyl 7-(4-acetyl-1-piperazinyl)-6-chloro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate, 12 ml of 5% hydrochloric acid and 3 ml of ethanol was heated with stirring on an oil bath of 100-110°C for 2 hours. After cooling to about 50°C, this was neutralized with 5% ammonia water. Crystals separated out were collected by filtration, washed with water, dried and recrystallized from N,N-dimethylformamide to give pale yellow crystals, m.p. not lower than 300°C (decompn). Elementary analysis calculated for C₁₆H₁₆ClN₃O₃S.1.5 H₂O: Calcd (%): C 48.92, H 4.87, N 10.70 Found (%): C 49.27, H 4.46, N 10.66 NMR δ (DMSO-d₆): 2.10 (d, 3H), 2.90 (m,4H), 3.10 (m,4H), 6.40 (q, 1H), 7.01 (s,1H), 8.10 (s, 1H), 8.00-9.00 (br, 1H). Example 4. Ethyl 6-fluro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. Ethyl 6,7-difluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate (5.0 g) was suspended in 150 ml of N,N-dimethylformamide and the suspension was stirred with 4.6 g of piperazine at room temperature for 48 hours. N,N-Dimethylformamide was evaporated in vacuo and to the residue was added ice water to collect the crystals by filtration. Purification by a column chromatography (silica gel/chloroform-methanol (1:1)) gave 3.0 g of desired compound, m.p. 224°C (decompn,). Elementary analysis calculated for C₁₈H₂₀FN₃O₃S.3/4 H₂O. Calcd (%): C 55.30, H 5.54, N 10.74 Found (%): C 55.29, H 5.52, N 10.37. Example 5. Ethyl 7-(4-acetonyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. Ethyl 6-fluoro-1-methyl-7-(1-piperazinyl)-4-oxo-4H-[1,3]-thiaceto[3,2-a]quinoline-3-carboxylate (3.8 g) was suspended in 50 ml of N,N-dimethylformamide, 1.66 g of potassium carbonate was added thereto, 1.65 g of bromoacetone was dropped in with ice cooling and stirring, and the mixture was stirred at room temperature for 20 hours. The reaction solution was poured over into ice water and the crystals separated out were collected by filtration, washed with water, dried and recrystallized from ethanol to give 3.7 g of desired compound in colorless powdery crystals, m.p. 196-200°C (decompn.). Elementary analysis calculated for C₂₁H₂₄FN₃O₄S: Calcd (%): C 58.18, H 5.58, N 9.69 Found(%): C 57.93, H 5.39, N 9.46 Example 6. 7-(4-Acetyl-1-piperazinyl)-6-fluro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. (1) Sodium salt of ethyl 3-(4-acetyl-1-piperazinyl)-4-fluorophenylaminomercaptomethylenemalonate. In 5 ml of dry tetrahydrofuran was suspended 0.173 g of sodium hydride, 0.57 g of diethyl malonate was dropped in with cooling and stirring, and the mixture was stirred for 30 minutes at room temperature. To this was added a solution of 1.0 g of 3-(4-acetyl-1-piperazinyl)-4-fluorophenyl isothiocyanate in 5.0 ml of dry tetrahydrofuran and the mixture was stirred at room temperature. After the reaction, the reaction solution was concentrated in vacuo and washed with ether to give 1.93 g of hygroscopic powder. (2) Ethyl 3-[3-(4-acetyl-1-piperazinyl)-4-fluorophenyl]-[1,3]-thiazetidin-2-ylidenemalonate. The powder (1.66 g) obtained in (1) was dissolved in 10 ml of dry tetrahydrofuran. The solution was dropped into a solution of 1.16 g of methylene iodide and 0.60 g of potassium carbonate in 20 ml of N,N-dimethylformamide with stirring. The mixture was stirred at about 60°C for 2 hours. The reaction solutions was diluted with ice water and extracted with ethyl acetate. The extract was washed with water, dried and concentrated followed by crystallizing from ether to give 810 mg of colorless crystals, m.p. 136-137°C. (3) 7-(4-Acetyl-1-poperazinyl)-6-fluro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid and ethyl 7-(4-acetyl-1-pierazinyl)-6-fluoro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. The crystals (800 mg) obtained in the above (2) were mixed with 30 g of polyphosphoric acid (PPA) and heated at 120 o C for 1 hour with stirring. The reaction solution was poured over into ice water and the crystals separated out were collected by filtration and washed with water and air-dried. Purification by a column chromatography (silica gel, chloroform-methanol (40:1 → 4:1)) gave 130 mg of the carboxylic,acid (m.p. 247-249°C, decompn.) and 180 mg of ethyl ester (m.p. 278-280°C, decompn.). (4) 7-(4-Acetyl-1-piperazinyl)-6-fluoro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Crystals (670 mg) obtained in the above (2) were dissolved in 10g of fuming sulfuric acid with stirring and cooling and the solution was heated with stirring at 100° for 5 minutes. After cooling, the reaction product was poured over into ice, crystals separated out were collected by filtration, washed with water and dried to give 520 mg of colorless powdery crystals, m.p. 247-249°C (decompn.). Elementary analysis calculated for C₁₇H₁₆FN₃O₄S.1/2 H₂O: Calcd (%): C 52.84, H 4.43, N 10.87 Found (%): C 53.04, H 4.53, N 10.79 Example 7. 6-Fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Three grams of ethyl 6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate was suspended in 30 ml of mixed solution of 5% potassium hydroxide/tert-butanol-water (75:25) and the suspension was heated at 50°C for 4 hours with stirring. To this was added 30 to 40 ml of water to prepare homogeneous solution. The solution was, neutralized with acetic acid and extracted with chloroform to which small amount of methanol was added. The extract was dried, concentrated, and the resulting residue was recrystallized from ethanol to give desired product. Yield: 2.23 g. M.p. 216-217°C (decomp.). The following compounds were prepared by similar manner as given in Examples 1 to 7. 6-Fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. not lower than 300°C (decompn,). 3400, 2800, 1700, 1625, 1600, 1485, 1375, 1300, 1250, 1230, 1110, 1010, 890, 805. 1-Ethyl-6-fluro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 233-234°C (decompn.). 6-Fluoro-7-(1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Hydrochloride. M.p. not lower than 300°C (decompn.). 3500, 2700, 1690, 1620, 1500, 1390, 1270, 1115, 800. 6-Fluoro-7-morpholino-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 244-248°C (decompn.). Diphosphate m.p. 182-190°C (decompn.). 6-Fluoro-7-thiomorpholino-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 220-242°C (decompn.). 7-(4-Allyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 216-218°C (decompn.). 7-(4-Ethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Hydrofluoride, m.p. 211-214°C (decompn.). 6-Fluoro-7-(4-isopropyl-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 227-228°C (decompn.). 6-Fluoro-7-(4-(2-hyroxyethyl)-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 230-232°C (decompn.). 7-(3-Aminomethyl-1-pyrrolidinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 213°C (decompn.). 6-Fluoro-7-(3-hydroxy-1-pyrrolidinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 253°C (decompn.). Sulfate, m.p. 206-208°C (decompn.). 6-Fluoro-7-(imidazol-1-yl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p.248-251°C (decompn.). 6-Fluoro-7-(3-hydroxmethyl-1-pyrrolidinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-]quinoline-3-carboxylic acid. M.p. 268-270°C (decompn.). 7-(4-Acetyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 263-265°C (decompn.). 6-Fluoro-1-methyl-7-morpholino-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 250-252°C (decompn.). Sulfate, m.p. 192-198°C (decompn.). 6-Fluoro-1-methtyl-7-thiomorpholino-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 258-260°C (decompn.). Sulfate, m.p. 229-231°C (decompn.). 6-Fluoro-7-(4-methyl-1-homopiperazinyl)-1-methyl-4-oxo-4H-[1,3]-thiazeto[3,2-a]quinoline-3-carboxylic acid. M.p. 158-164°C (decompn.). 6-Fluoro-7-(3-hydroxypiperidino)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 255-257°C (decompn.). 7-(4-(4-aminobenzyl)-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p.209-211°C (decompn.). 6-Fluoro-7-(2-methylimidazol-1-yl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 282-284°C (decompn.). 6-Fluoro-7-(4-hydroxypiperidino)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 266-268°C (decompn.). 6-Fluoro-7-piperidino-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 246-248°C (decompn.). 6-Fluoro-1-methyl-7-(1-pyrrolindinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 275-277°C (decompn.). 6-Fluoro-7-(4-(4-methoxyphenyl)-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 258-260°C (decompn.). 6-Fluoro-7-(4-phenyl-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 267-269°C (decompn.). 6-Fluoro-7-(3-hydroxy -4-hydroxymethyl-1-pyrrolindyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 245-246°C (decompn.). 6-Fluoro-7-(3,4-di(2-hydroxyethyl)-1-pyrrolidinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Mp. 220-221°C (decompn.). 7-(N,N-Dihydroxyethylsmino)-6-fluro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 227-230°C (decompn). 6-Fluoro-1-methyl-7-(3-amino-1-pyrrolidinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 222-230°C (decompn.). Ethanesulfonate, m.p. 250-251°C (decampn.). d-10-Camphorsulfonate, m.p. 238-240°C (decompn.). 6-Fluoro-1-methyl-7-(4-methyl-1-piperidino)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 249-251°C (decompn.). 7-(3-Dimethylamino-1-pyrrolidinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Hydrochloride, m.p. 275-276°C (decompn.). 6-Fluoro-1-methyl-7-(3-oxo-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 254-256°C (decompn.). Sulfate, m.p.198-200°C (decompn.). 6-Fluoro-1-methyl-7-(3-methylamino-1-pyrrolidinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Hydrochloride, m.p. 213-216°C (decompn.). 6-Fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-8-nitro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Nitrate, m.p. not lower than 300°C (decompn.). 3400, 1700, 1620, 1595, 1530, 1480, 1450, 1380, 1270, 1125, 1070, 970, 800. 6-Fluoro-1-methyl-7-(4-methyl-3-oxo-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. Mp. 254-255°C (decompn.). 8-Amino-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 240°C (decompn.). Ethyl 8-chloro-6-fluro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 196-197°C. Ethyl 6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-8-nitro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 185-188°C (decompn.). Ethyl 6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 223-225°C. Ethyl 8-bromo-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 191-192°C (decompn.). 8-Chloro-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p.189-190°C (decompn.). 8-Bromo-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 198-200°C (decompn.). Ethyl 8-amino-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 200-205°C (decompn.). Ethyl 6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 260-265°C (decompn.). Ethyl 8-acetamido-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 192-195°C (decompn.). Ethyl 8-diacetylamino-6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 212-214°C (decompn.). 6-Fluoro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 215-218°C (decompn.). 1/2 Sulfate, m.p. 285°C (decompn.); hydrochloride, m.p. 295-300°C (decompn.); methanesulfonate, m.p. 236-239°C (decompn.); p-toluenesulfonate, m.p. 196-200°C (decompn.); maleate, m.p. 225-227°C (decompn.). Ethyl 7-dimethylamino-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 194°C Ethyl 6-fluoro-1-methyl-7-(3-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 113-135°C. Ethyl 6-fluoro-7-(4-isopropyl-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 230-231°C. Ethyl 1-ethyl-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 211-212°C. Ethyl 6-fluoro-7-(3-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. not lower than 300°C. (decompn.). 3400, 2950, 1705, 1625, 1595, 1500, 1370, 1320, 1250, 1155, 1050, 895, 850, 795. Ethyl 7-(3,4-dimethyl-1-piperazinyl)-6-fluoro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 262°C (decompn.). Ethyl 6-fluoro-1-methyl-7-morpholino-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 257-258°C (decompn.). Ethyl 7-(3,4-dimethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 204-206°C. Ethyl 6-fluoro-7-(3-hydroxymethyl-1-pyrrolidinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 230-233°C (decompn.). Ethyl 6-fluoro-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. not lower than 300°C (decompn.). 3400, 3260, 1705, 1625, 1595, 1540, 1500, 1370, 1325, 1250, 1160, 1090, 1040, 795. Ethyl 6-fluoro-1-methyl-7-(4-methyl-3-oxo-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 195-198°C. Ethyl 6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-1-phenyl-4H-[1,3]-thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 208-209°C. Ethyl 6-fluoro-4-oxo-1-phenyl-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 189-191°C (decompn.). Ethyl 6-fluoro-7-(3-methyl-1-piperazinyl)-4-oxo-4H-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 153°C. Ethyl 7-(3,4-dimethyl-1-piperazinyl)-6-fluoro-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 165°C Isopropyl 6-fluoro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 200°C (decompn.). 7-(3,4-Dimethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto-[3,2-a]quinoline-3-carboxylic acid. M.p. 198-201°C (decompn.). Formate, m.p. 220 o C (decompn.). Methyl 6-fluoro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 208°C (decompn.). 6-Chloro-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 235 -240°C (decompn.). 6-Chloro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 220-230°C (decompn.). 6-Chloro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 257-259°C (decompn.). Hydrochloride, m.p. 250-255°C (decompn.). Sulfate, m.p. 225-230°C (decompn.). Ethyl 7-(4-acetonyl-3-methyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 190-192°C. Methyl 7-(3,4-dimethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 199-204°C (decompn.). Isopropyl 7-(3,4-dimethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 194-197°C (decompn.). Ethyl, 7-(4-acetonyl-1-piperazinyl)-6-fluoro-1-phenyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 190-191°C (decompn.). 7-(4-Acetonyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 208-209°C (decompn.). Hydrochloride, m.p. 255-257 o C (decompn.); methanesulfonate, m.p. 215-218 o C (decompn.); maleate, m.p. 156-158 o C (decompn.); p-toluenesulfonate, m.p. 245-248 o C (decompn.); sodium salt, m.p.>300 o C (decompn.). Ethyl 7-(4-acetonyl-1-piperazinyl)-6-fluoro-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 248-250°C (decompn.). 6-Fluoro-1-methyl-7-(3-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 170-172°C (decompn.). Ethyl 6-fluoro-methoxy-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 213°C (decompn.). Glycerol 6-fluoro-1-methyl-7-(1-piperazinyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 155-160°C. n-Butyl 6-fluoro-1-methyl-7-(1-piperazinyl)-4-oxo-4H-[1,3]-thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 212-213°C (decompn.). Ethyl 6-fluoro-1-methyl-7-(4-(3-methyl-2-oxobutyl)-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 183°C. Ethyl 1-(4-chlorophenyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 142-143°C. 3-Hydroxypropyl 6-fluoro-1-methyl-7-(1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 208-209°C (decompn.). Methoxyethyl 6-fluoro-1-methyl-7-(1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 198-201°C(decompn.). Hydroxyethyl 6-fluoro-1-methyl-7-(1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 164-168° (decompn.). Ethyl 6-fluoro-1-methyl-4-oxo-7-(4-(2-oxo-2-phenylethyl)-1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 203-205°C (decompn.). Ethyl 7-(4-(3,3-dimethyl-3-oxobutyl)-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 180-182°C (decompn.). Ethyl 6-fluro-1-methyl-4-oxo-4H-7-(4-(3-oxobutyl)-1-piperazinyl)-4H-[1,3]thiaceot[3,2-a]quinoline-3-carboxylate. M.p. 169-171°C (decompn.). Ethyl 6-fluoro-1-methyl-4-oxo-7-(4-trifluoroacetyl-1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 241-242°C (decompn.). Hydroxyethyl 7-(4-ethyl-1-piperazinyl-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 159-162°C (decompn.). Ethyl 7-(4-ethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 236-238°C (decompn.). Ethyl 6-fluoro-7-(4-(2-methoxyethyl)-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 188-189°C. Ethyl 7-(4-carboethoxymethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 205-207°C (decompn.). Ethyl 6-fluoro-7-(4-hydroxyethyl-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 215-216°C (decompn.). Ethyl 7-(4-cyanomethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 252-253°C (decompn.). Ethyl 6-fluoro-1-methyl-7-(4-(2-propynyl)-1-piperazinyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 209-210°C (decompn.). Ethyl 6-fluoro-7-morpholino-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a] quinoline-3-carboxylate. M.p. 211°C Ethyl 6-fluoro-7-(4-(2-hydroxyethoxyethyl)-1-piperazinyl)-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 196-198°C. 6-Fluoro-1-(4-fluorophenyl)-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 298-304°C (decompn.). Ethyl ester, m.p. 192°C (decompn.). 1-(2,4-Difluorophenyl)-6-fluoro-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. >300°C (decompn.). Ethyl ester, m.p. 180°C (decompn.). 1-(2,5-Difluorophenyl)-6-fluoro-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 280-284°C (decompn.). 6-Fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 216-217°C (decompn.). Hydrochloride, m.p. 270°C (decompn.); methanesulfonate, m.p. 205- 210°C (decompn.); p-toluenesulfonate, m.p. 160-166°C; maleate, m.p.108-182°C (decompn.); sodium salt, m.p. 290-300°C (decompn.). 6-Fluoro-4-oxo-1-phenyl-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxyilc acid. M.p. 210-230°C (decompn.); hydrochloride, m.p. 220°C (decompn.); p-toluenesulfonate, m.p. 200-205°C (decompn.); maleate, m.p. 210°C (decompn.). Pivaloyloxymethyl 6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 169-172°C. Pivaloyloxymethyl 7-(4-acetonyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 132-135°C. 6-Fluoro-8-methoxy-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxyic acid. M.p. 220°C (decompn.). 6-Fluoro-8-methoxy-7-(4-methyl-1-piperazinyl)-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 176-177°C. Ethyl ester, m.p. 96-98°C (decompn.). Ethyl 6-fluoro-8-methoxy-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 219°C (decompn.). 1-(2,4-Difluorophenyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 209-211°C (decompn.). Ethyl ester, m.p. 212-214°C. 1-(2,5-Difluorophenyl)-6-fluoro-7-(4-fluoro-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 213-215°C (decompn.). Ethyl ester, m.p. 218-220°C. 1-(2,6-Difluorophenyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 229-232°C (decompn.). Ethyl ester, m.p. 144-147°C (decompn.). 1-(3,4-Difluorophenyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 277-280°C (decompn.). Ethyl ester, m.p. 170-173°C. 6-Fluoro-1-(4-fluorophenyl)-7-(-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 208-215°C (decompn.). Ethyl ester, m.p. 121-122°C. 6-Fluoro-1-(3-fluorophenyl)-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 222-225°C (decompn.). Ethyl ester, m.p. 180-183°C. 6-Fluoro-1-(2-fluorophenyl)-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxyic acid. M.p. 203-205°C (decompn.). Ethyl ester, m.p. 198-201°C. 6-fluoro-1-(4-methylphenyl)-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 191-193°C (decompn.). Ethyl ester, m.p. 156-160°C. 6-Fluoro-1-(2-methylphenyl)-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 185-187°C (decompn.). Ethyl ester, m.p. 162-165°C. Ethyl 6-fluoro-7-(4-methyl-1-piperazinyl)-1-(4-nitrophenyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate. M.p. 228-231°C (decompn.). 6-Fluoro-7-(4-methyl-1-piperazinyl)-1-(4-trifluorophenyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 239-242°C (decompn.). Ethyl ester, m.p. 200-203°C. 6-Fluoro-7-(4-methyl-1-piperazinyl)-1-(3-trifluorophenyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. M.p. 223-226°C (decompn.). Ethyl ester, m.p. 206-209°C. Test Example. As hereinafter, the result of antibacterial experiments of the representative examples of the present invention compounds on their utility is given. 1. Measurement of minimum inhibitory concentration (MIC). Test method: The MIC was measured by an agar plate dilution method in accordance with a standard method of Japan Chemotherapeutic Society (cf. Nippon Kagaku Ryoho Gakkaishi, 29(1), 76-79, 1981). Thus, the organisms incubated at 37°C for 18 hours using sensitivity test broth (Nissei) was diluted to 10⁶ CFU/ml using the same broth. This was inoculated, using a microplanter, to a drug-containing sensitivity test agar (Nissei), cultured at 37 o C for 18 hours, and the MIC was measured. As a control, enoxacin and ofloxacin were used in Tests 1 and 2, respectively. The result is given in Table 1. The present invention compounds exhibited very strong antibacterial activity against Gram-positive and negative bacteria including Pseudomonas aeruginosa. The compounds of the present invention in the above table are as given below. (1): 6-Fluoro-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (2): 6-Fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (3): 6-Fluoro-7-morpholino-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (4): 7-(4-Allyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (5): 6-Fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid sulfate; (6): 6-Fluoro-1-methyl-7-thiamorpholino-4-oxo-4H-[1,3]-thiaceto[3,2-a]quinoline-3-carboxylic acid sulfate; (7): 6-Chloro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (8): 6-Fluoro-1-methyl-7-(3-amino-1-pyrrolidinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid ethanesulfonate; (9): 8-Amino-6-fluro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (10): 7-(4-(4-Aminobenzyl)-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (11): 6-Fluoro-4-oxo-1-phenyl-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (12): 6-Fluoro-1-(4-flurophenyl)-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (13): 6-Fluro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; and (14): 6-Fluoro-8-methoxy-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. The numbers of the inoculated bacteria were 10⁶ in all cases and the unit of the MIC was micrograms/ml. 2. Therapeutic Effect on Infection in Mice. Test Method: Escherichia coli KC-14 and Psudomonas aeruginosa E-2 were suspended in 4% mucin and 0.25 ml of it was inoculated into peritoneal cavity of ddY-strain male mice (body weight about 20 g; 4 weeks age; 10 mice per group). The numbers of the inoculated bacteria were 5.1 x 10⁴ CFU/mouse and 7.5 x 10⁴ CFU/mouse for E. coli and P. aeruginosa, respectively. The drug was administered orally once 2 hours after the infection of the bacteria and, out of the survival rate after one week, the ED₅₀ values were calculated by a Behrens-Karber method. As controls, ofloxacin and enoxacin were used. The result is given in Table 2. Compound ED₅₀ (mg/kg) (1) (2) Ofloxacin 0.8 16.0 Enoxacin 1.8 13.1 (1) 0.5 4.2 (2) 0.5 4.0 (3) 0.6 5.0 (4) 0.5 4.4 (5) 0.4 4.5 (6) 0.6 5.0 (7) 0.5 4.0 Bacteria used for calculating ED₅₀ were E. coli KC-14 for (1) and P. aeruginosa E-2 for (2). The compounds of the present invention used were as follows. (1): 6-Fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; (2): Ethyl 6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; (3): Ethyl 7- (3,4-dimethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; (4): Ethyl 6-fluoro-7-(4-methyl-1-piperazinyl-4-oxo-1-phenyl-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; (5): Ethyl 7-(4-acetonyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; (6): 7-(4-Acetonyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; and (7): 6-Fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid sulfate. Compounds of the present invention showed strong therepeutic effect to infectious diseases to mice. They especially showed stronger activity to P. aeruginosa than enoxacin which has been said to exhibit strong action thereto. 3. Acute Toxicity: The result of oral administration to ddY-strain male mice (7 weeks age; body weight ca. 35 g; 3 mice per group) for 2 weeks was given. All of the present invention compounds tested showed low toxicity. For instance, LD₅₀ values of the following compounds were not lower than 3 g/kg at all times. Thus, 6-fluoro-1-methyl-4-oxo-7-(1-piperazinyl)-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid; ethyl 7-(3,4-dimethyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; ethyl 7-(4-acetonyl-1-piperazinyl)-6-fluoro-1-methyl-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylate; and 6-fluoro-1-methyl-7-(4-methyl-1-piperazinyl)-4-oxo-4H-[1,3]thiaceto[3,2-a]quinoline-3-carboxylic acid. (Effect) It is quite apparent from the afore-mentioned facts that the compounds of the present invention are effective in much less doses than those of the conventional antibacterials to not only P. aeruginosa but also both Gram-positive and negative bacteria and exhibit antibacterial spectra of wide range. Many of the conventional pharmaceuticals are in free carboxylic acid type and, in general, their bioavailability is not satisfactory. Accordingly, it is often observed that they do not show so much therapeutic effect to infectious diseases as expected from their antibacterial activity in vitro. Both carboxylic acid type and ester type of the compounds of the present invention showed therapeutic effect to infectious diseases in the test in vivo. Compounds of the ester type exhibited especially good absorbability after oral administration, maintained high blood levels for long time, and showed sufficient therepeutic effect. In addition, toxicity of the compounds of the present invention are quite low. Accordingly, the compounds of the present invention can be safely used to mammalian animals including human being as a therapeutic agent for both systemic diseases and local diseases such as, for example, infectious diseases in urinary tract and bilary tract.

Claims: Claims for the following Contracting States : DE, SE Quinolinecarboxylic acid derivatives represented by the following general formula (I) and physiologically acceptable salts thereof wherein R¹ is hydrogen, alkyl having 1 to 6 carbon atoms or phenyl unsubstituted or substituted with alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, trifluoromethyl or nitro; R² is hydrogen, alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, nitro or amino unsubstituted or substituted with acyl having 2 to 3 carbon atoms; R³ is hydrogen or alkyl having 1 to 6 carbon atoms unsubstituted or substituted with hydroxy, acyloxy having 2 to 6 carbon atoms or alkoxy having 1 to 4 carbon atoms; R⁴ and R⁵ are same or different and are alkyl having 1 to 4 carbon atoms or hydroxyalkyl having 1 to 4 carbon atoms or R⁴ and R⁵ may be combined and may form a 4- to 8-membered cyclic amino together with the adjacent nitrogen atom, selected from the group consisting of azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino und pyrazolino, and said cyclic amino may also be substituted with 1 to 3 substituents selected from the group consisting of alkyl having 1 to 6 carbon atoms, alkenyl having 2 to 6 carbon atoms, alkynyl having 2 to 6 carbon atoms, aryl having 6 to 12 carbon atoms, aralkyl having 7 to 12 carbon atoms, hydroxyalkyl having 1 to 4 carbon atoms, alkoxyalkyl having 2 to 6 carbon atoms (said alkoxyalkyl optionally substituted by hydroxyl), aminoalkyl having 1 to 4 carbon atoms, cyanoalkyl having 2 to 4 carbon atoms, carboethoxymethyl, acylalkyl having 3 to 8 carbon atoms, acyl having 1 to 6 carbon atoms, hydroxyl, oxo, amino, alkylamino having 1 to 4 carbon atoms or dialkylamino having 1 to 4 carbon atoms and X is halogen. A method of manufacturing quinolinecarboxylic acid derivatives represented by the general formula (I) and physiologically-acceptable salts thereof wherein R¹ R², R³, R⁴, R⁵ and X defined as in claim 1 characterized in that a compound of the general formula (III) (wherein R⁶ is alkyl; A is fluorine or and meanings of other symbols are the same as those already defined) is made to react with a dihalogeno compound of the formula (wherein Y and Z are the same or different and are halogen and R¹ is the same as that already defined) and, in case A is fluorine, an amine of the formula (meanings of R⁴ and R⁵ are the same as those already defined) is then made to react therewith and, at the desired stage in the above course, hydrolysed if necessary. A method of manufacturing a compound of the general formula (IXa) (wherein definitions of the symbols are the same as those defined in Claim 2), characterized in that, a compound of the formula (VIIIa) is made to react with a dihalogeno compound of the formula (wherein definitions of the symbols are the same as those defined in Claim 2). A method of manufacturing quinolinecarboxylic acid derivatives of the general formula (I) and physiologically-acceptable salt thereof (wherein definitions of the symbols are the same as those defined in Claim 2), characterized in that, a compound of the formula (IXa) (wherein definitions of the symbols are the same as those defined in Claim 2) is subjected to a ring closure and, in case A is fluorine, it is then condensed with an amine of the formula (wherein definitions of the symbols are the same as those defined in Claim 2) and, at the desired stage during the above course, hydrolysed if necessary. A pharmaceutical composition useful for treating bacterial or fungal infections in humans and animals which comprises a therapeutically effective amount of a compound according to Claim 1. Claims for the following Contracting State : AT Quinolinecarboxylic acid derivatives represented by the following general formula (I) and physiologically acceptable salts thereof wherein R¹ is hydrogen, alkyl having 1 to 6 carbon atoms or phenyl unsubstituted or substituted with alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, trifluoromethyl or nitro; R² is hydrogen, alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, nitro or amino unsubstituted or substituted with acyl having 2 to 3 carbon atoms; R³ is hydrogen or alkyl having 1 to 6 carbon atoms unsubstituted or substituted with hydroxy, acyloxy having 2 to 6 carbon atoms or alkoxy having 1 to 4 carbon atoms; R⁴ and R⁵ are same or different and are alkyl having 1 to 4 carbon atoms or hydroxyalkyl having 1 to 4 carbon atoms or R⁴ and R⁵ may be combined and may form a 4- to 8-membered cyclic amino together with the adjacent nitrogen atom, selected from the group consisting of azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino und pyrazolino, and said cyclic amino may also be substituted with 1 to 3 substituents selected from the group consisting of alkyl having 1 to 6 carbon atoms, alkenyl having 2 to 6 carbon atoms, alkynyl having 2 to b carbon atoms, aryl having 6 to 12 carbon atoms, aralkyl having 7 to 12 carbon atoms, hydroxyalkyl having 1 to 4 carbon atoms, alkoxyalkyl having 2 to 6 carbon atoms, aminoalkyl having 1 to 4 carbon atoms, cyanoalkyl having 2 to 4 carbon atoms, carboethoxymethyl, acylalkyl having 3 to 8 carbon atoms, acyl having 1 to 6 carbon atoms, hydroxyl, oxo, amino, alkylamino having 1 to 4 carbon atoms or dialkylamino having 1 to 4 carbon atoms; and X is halogen. Quinolinecarboxylic acid derivatlves represented by the following general formula (I) and physiologically acceptable salts thereof wherein R¹ is hydrogen, alkyl having 1 to 6 carbon atoms or phenyl unsubstituted or substituted with alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, trifluoromethyl or nitro; R² is hydrogen, alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, nitro or amino unsubstituted or substituted with acyl having 2 to 3 carbon atoms; R³ is hydrogen or alkyl having 1 to 6 carbon atoms unsubstituted or substituted with hydroxy, acyloxy having 2 to 6 carbon atoms or alkoxy having 1 to 4 carbon atoms; R⁴ and R⁵ are same or different and are alkyl having 1 to 4 carbon atoms or hydroxyalkyl having 1 to 4 carbon atoms or R⁴ and R⁵ may be combined and may form a 4- to 8-membered cyclic amino together with the adjacent nitrogen atom, selected from the group consisting of azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino und pyrazolino, and said cyclic amino may also be substituted with 1 to 3 substituents selected from the group consisting of alkyl having 1 to 6 carbon atoms, alkenyl having 2 to 6 carbon atoms, alkynyl having 2 to 6 carbon atoms, aryl having 6 to 12 carbon atoms, aralkyi having 7 to 12 carbon atoms, hydroxyalkyl having 1 to 4 carbon atoms, hydroxyl-substituted alkoxyalkyl having 2 to 6 carbon atoms, aminoalkyl having 1 to 4 carbon atoms, cyanoalkyl having 2 to 4 carbon atoms, carboethoxymethyl, acylalkyl having 3 to 8 carbon atoms, acyl having 1 to 6 carbon atoms, hydroxyl, oxo, amino, alkylamino having 1 to 4 carbon atoms or dialkylamino having 1 to 4 carbon atoms; and X is halogen. A method of manufacturing quinolinecarboxylic acid derivatives represented by the general formula (I) and physiologically-acceptable salts thereof wherein R¹ is hydrogen, alkyl having 1 to 6 carbon atoms or phenyl unsubstituted or substituted with alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, trifluoromethyl or nitro; R² is hydrogen, alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, nitro or amino unsubstituted or substituted with acyl having 2 to 3 carbon atoms; R³ is hydrogen or alkyl having 1 to 6 carbon atoms unsubstituted or substituted with hydroxy, acyloxy having 2 to 6 carbon atoms or alkoxy having 1 to 4 carbon atoms; R⁴ and R⁵ are same or different and are alkyl having 1 to 4 carbon atoms or hydroxyalkyl having 1 to 4 carbon atoms or R⁴ and R⁵ may be combined and may form a 4- to 8-membered cyclic amino together with the adjacent nitrogen atom, selected from the group consisting of azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino und pyrazolino, and said cyclic amino may also be substituted with 1 to 3 substituents selected from the group consisting of alkyl having 1 to 6 carbon atoms, alkenyl having 2 to 6 carbon atoms, alkynyl having 2 to 6 carbon atoms, aryl having 6 to 12 carbon atoms, aralkyl having 7 to 12 carbon atoms, hydroxyalkyl having 1 to 4 carbon atoms, alkoxyalkyl having 2 to 6 carbon atoms, aminoa]kyl having 1 to 4 carbon atoms, cyanoalkyl having 2 to 4 carbon atoms, carboethoxymethyl, acylalkyl having 3 to 8 carbon atoms, acyl having 1 to 6 carbon atoms, hydroxyl, oxo, amino, alkylamino having 1 to 4 carbon atoms or dialkylamino having 1 to 4 carbon atoms; and X is halogen, characterized in that a compound of the general formula (III) (wherein R⁶ is alkyl; A is fluorine or and meanings of other symbols are the same as those already defined) is made to react with a dihalogeno compound of the formula (wherein Y and Z are the same or different and are halogen and R¹ is the same as that already defined) and, in case A is fluorine, an amine of the formula (meanings of R⁴ and R⁵ are the same as those already defined) is then made to react therewith and, at the desired stage in the above course, hydrolysed if necessary. A method of manufacturing quinolinecarboxylic acid derivatives represented by the general formula (I) and physiologically-acceptable salts thereof wherein R¹ is hydrogen, alkyl having 1 to 6 carbon atoms or phenyl unsubstituted or substituted with alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, trifluoromethyl or nitro; R² is hydrogen, alkyl having 1 to 6 carbon atoms, alkoxy having 1 to 4 carbon atoms, hydroxy, halogen, nitro or amino unsubstituted or substituted with acyl having 2 to 3 carbon atoms; R³ is hydrogen or alkyl having 1 to 6 carbon atoms unsubstituted or substituted with hydroxy, acyloxy having 2 to 6 carbon atoms or alkoxy having 1 to 4 carbon atoms; R⁴ and R⁵ are same or different and are alkyl having 1 to 4 carbon atoms or hydroxyalkyl having 1 to 4 carbon atoms or R⁴ and R⁵ may be combined and may form a 4- to 8-membered cyclic amino together with the adjacent nitrogen atom, selected from the group consisting of azetidino, pyrrolidino, piperidino, azepino, azocino, piperazino, homopiperazino, pyrrolino, morpholino, thiomorpholino, imidazolino, imidazolidino, imidazolinino, pyrazolidino and pyrazolino, and said cyclic amino may also be substituted with 1 to 3 substituents selected from the group consisting of alkyl having 1 to 6 carbon atoms, alkenyl having 2 to 6 carbon atoms, alkynyl having 2 to 6 carbon atoms, aryl having 6 to 12 carbon atoms, aralkyl having 7 to 12 carbon atoms, hydroxyalkyl having 1 to 4 carbon atoms, hydroxyl-substituted alkoxyalkyl having 2 to 6 carbon atoms, aminoalkyl having 1 to 4 carbon atoms, cyanoalkyl having 2 to 4 carbon atoms, carboethoxymethyl, acylalkyl having 3 to 8 carbon atoms, acyl having 1 to 6 carbon atoms, hydroxyl, oxo, amino, alkylamino having 1 to 4 carbon atoms or dialkylamino having 1 to 4 carbon atoms; and X is halogen, characterized in that a compound of the general formula (III) (wherein R⁶ is alkyl; A is fluorine or and meanings of other symbols are the same as those already defined) is made to react with a dihalogeno compound of the formula (wherein Y and Z are the same or different and are halogen and R¹ is the same as that already defined) and, in case A is fluorine, an amine of the formula (meanings of R⁴ and R⁵ are the same as those already defined) is then made to react therewith and, at the desired stage in the above course, hydrolysed if necessary. A method of manufacturing a compound of the general formula (IXa) (wherein definitions of the symbols are the same as those defined in Claim 3 and 4), characterized in that, a compound of the formula (VIIIa) is made to react with a dihalogeno compound of the formula (wherein definitions of the symbols are the same as those defined in Claim 3 and 4). A method of manufacturing quinolinecarboxylic acid derivatives of the general formula (I) and physiologically-acceptable salt thereof (wherein definitions of the symbols are the same as those defined in Claim 3 and 4), characterized in that, a compound of the formula (IXa) (wherein definitions of the symbols are the same as those defined in Claims 3 and 4) subjected to a ring closure and, in case A is fluorine, it is then condensed with an amine of the formula (wherein definitions of the symbols are the same as those defined in Claim 3 and 4) and, at the desired stage during the above course, hydrolysed if necessary. A pharmaceutical composition useful for treating bacterial or fungal infections in humans and animals which comprises a therapeutically effective amount of a compound according to Claim 1 or 2.

Inventors: KAZUNO, KENJI, KISE, MASAHIRO, KITANO, MASAHIKO, OZAKI, MASAKUNI, SEGAWA, JUN ROOM 405, SHIRAHASE, ICHIRO, TOMII, YOSHIFUMI
Applicants: NIPPON SHINYAKU COMPANY, LIMITED
Application year: 1987
Main classification: C07D513
IPC codes: A61P31, C07D513, A61K31