QUINOLINE AMIDES

Abstract: Compounds of the formula: (wherein Ra represents an optionally substituted alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkylalkyl, aryl, aralkyl or heteroaryl group; Rb and Rc, which may be the same or different, each represents hydrogen or an optionally substituted alkyl, cyclo­ alkyl, cycloalkylalkyl, alkenyl, alkynyl, phenyl or benzyl group, or Rb and Rc together with the nitrogen atom therebetween represent a saturated nitrogen containing heterocyclic ring; and Rd represents a hydrogen atom or an alkyl or alkanoyl group) and the salts thereof are useful as agents for binding to benzodiazepine receptors, and especially possess anxiolytic activity.

Description: Summary of the Invention The compounds of the invention are 4-amino-quinoline-3-carboxamides which are useful as agents for binding to benzodiazepine receptors. Detailed Description of the Invention The compounds of the invention are 4-amino-quinoline-3-carboxamides of formula I: (Formula set out on pages following Examples) wherein Ra is selected from a group consisting of (2-6C)alkyl, (3-7C)cycloalkyl, (4-7C)cycloalkylalkyl, (3-6C)alkenyl, (3-6C)alkynyl, (6-10C)aryl, benzyl, (3-6C)haloalkyl having at least one halo group wherein the halo group(s) B selected from chloro and fluoro, (3-6C)hydroxyalkyl, (4-7C)hydroxycycloalkylalkyl, and 3-methyl-2-thienyl; Rb and Rc are independently selected from a group consisting of hydrogen, (2-6C)alkyl, (3-7C)cycloalkyl, (4-7C)cycloalkylalkyl, (3-6C)alkenyl, (3-6C)alkynyl, (2-6C)alkoxyalkyl, (2-6C)haloalkyl having at least one halo group (wherein the halo group(s) is selected from chloro and fluoro), and(3-6C)haloalkenyl having at least one halo group(wherein the halo group is selected from chloro and fluoro) and where the halo group(s) is on a carbon having a double bond; Rd is selected from a group consisting of hydrogen, (1-6C)alkyl and (1-6C)alkanoyl; and the pharmaceutically acceptable acid addition salts thereof. Particular values for Rd are hydrogen, (2-4C) alkanoyl such as acetyl, propionyl and butyryl. More particular values for the above defined groups are as follows: Ra : ethyl, propyl, butyl, pentyl, 3-pentynyl and 2-methylpropyl; Rb : propyl, 2-propenyl, cyclopropylmethyl, 2,2,2-trifluoroethyl, 2-chloro-2-propenyl, and 3,3-dichloro-2-propenyl; Rc : hydrogen; and Rd : hydrogen. A particular pharmaceutically acceptable acid addition salt is one formed with hydrochloric, hydrobromic, sulfuric, nitric, phosphoric or an alkane sulfonic acid such as methanesulfonic acid. Preferred compounds of the invention in order of preference are a) 4-amino-8-butylquinoline-3-[N-(2-propenyl)]carboxamide (Example 7); and b) 4-amino-8-butylquinoline-3-[N-propyl]carboxamide (Example 6). It will be appreciated that certain of the compounds of this invention may contain an asymmetrically substituted carbon atom and may exist in, and be isolated in, optically-active and racemic forms. In addition, it will be appreciated that certain compounds of this invention may contain a double bond and may exist in, and be isolated in, separate stereoisomeric forms (′E′ and ′Z′) about that group. Some compounds may exist in more than one tautomeric form. Some compounds may exhibit polymorphism. It is to be understood that the present invention encompasses any racemic, optically-active, tautomeric, polymorphic or stereoisomeric form, or mixtures thereof, which form possesses the utilities described herein, it being well known in the art how to prepare optically-active forms (for example, by resolution of the racemic form or by synthesis from optically-active starting materials) and individual ′E′ and ′Z′ stereoisomers (for example, by chromatographic separation of a mixture thereof) and how to determine the properties described herein by the standard tests described hereinafter. In this specification Ra, Rb, Rc, Rd, et cetera stand for generic radicals and have no other significance. It is to be understood that the generic term "(1-6C)alkyl" includes both straight and branched chain alkyl radicals but references to individual alkyl radicals such as "propyl" embrace only the straight chain ("normal") radical, branched chain isomers such as "isopropyl" being referred to specifically. A similar convention applies to other generic groups, for example, "alkylene" and "alkenylene" et cetera . Halogeno is fluoro, chloro, bromo or iodo. Compounds of formula I may be prepared using methods known for the preparation of chemically similar compounds. Thus the following processes are provided as further features of the invention where Ra, Rb, Rc and Rd have the definitions stated above unless indicated otherwise: (a) reaction of a compound of formula II: (Formula set out on pages following Examples) II where A is a carboxylic acid (-COOH) or activated derivative thereof, for example ester, acid chloride (preferred), acid anhydride or acyl imidazolide, with an amine of formula RbRcNH; (b) reaction of a compound of formula IIa: (Formula set out on pages following Examples) IIa where A¹ is an ester having 1-6 carbons (for example, -CO₂CH₂CH₃ or -CO₂CH₃), with an aluminum reagent of the formula Re₂AlNRbRc or ReAl(Cl)NRbRc wherein Re is methyl, ethyl, propyl, or isobutyl; (c) reaction of a compound of formula III: (Formula set out on pages following Examples) III where X is iodo or bromo, with an organometallic reagent of formula RaMX or Ra₂M, wherein M is magnesium or zinc, in the presence of a palladium catalyst (for example, dichloro[1,1′-bis(diphenylphosphino)ferrocene]palladium (II)). (d) for a compound of formula I in which Rd is alkanoyl, acylation of a corresponding compound of formula I in which Rd is hydrogen. Both processes (a) and (b) are preferred methods. Process (b) is based in part on the work of S. M. Weinreb, et al., Synthetic Communications , (1982) 12 : 989-993, which describes direct conversion of esters to carboxanides employing aluminum amide reagents. The starting material for processes (a) and (b) may be prepared from a compound of formula IV: (Formula set out on pages following Examples) IV where A¹ has the value as defined above, by first converting the hydroxyl group to a suitable leaving group, examples being chloro, bromo and ethoxy, followed by displacement with an amine of formula RdNH₂ to give a compound of formula IIa. Either direct conversion to compounds of formula I by process (b) or conversion to the acid by hydrolysis of the ester to then use process (a) can be accomplished. The starting material of formula IV where A¹ is -CO₂CH₂CH₃ or -CO₂CH₃ may be formed according to the procedure of R. G. Gould et al., J. Amer. Chem. Soc .,(1939) 61 : 2890-2895 which describes the synthesis of such components from anilines. For the processes of this invention the use of ortho-substituted anilines give the desired compounds. All of the anilines used in the examples were commercially available except for 2-pentylaniline and 2-butylaniline. 2-Pentylaniline and 2-butylaniline may be made by methods well documented in the literature, for example see P. G. Gassmann et al., J. Amer. Chem. Soc. , (1974) 96 : 5487-95; and R. Sikkar et al. Acta Chemica Scandinavica , (1980) Vol. B34 , pp. 551 557 respectively. For process (c) a compound of formula IIb: where A¹ has a value as defined above and X is iodo or bromo, may be converted to the corresponding carboxamide of formula III according to the procedure of process (b) using the aluminium amide reagents. Subsequent organometallic cross-coupling then may be achieved by process (c) paralleling the chemistry of T. Hayashi et al, J. Amer. Chem. Soc. , (1984) 106 : 158-163. Compounds of formula IIb may be made from corresponding orthhatoanilines using the procedures described for formula IIa. Pharmaceutically acceptabte acid addition salts may be formed by reacting a compound of formula I with an appropriate acid, for example, by dissolving a compound of formula I, adding a selected acid to the solution and recovering the salt. Compounds capable of binding to benzodiazepine receptors are known to possess a spectrum of activities which range from anxiolytic activity to the activity of reversing the action of benzodiazepines in the central nervous system. In generat the compounds of the present invention are believed to possess anxiolytic activity. It will be appreciated however that compounds may vary in their activity depending on chemical structure and thus compounds of the present invention may possess a varying ratio of such above-mentioned activities. This may be demonstrated in the Shock-Induced Suppression of Drinking (Rats) Test (SSD) described in Pharmacology Biochemistry and Behaviour , (1980) 12 : 819-821. This test may be carried out as follows : Male rats in the weight range of 200 to 220 g deprived of water for 48 hours and deprived of food for 24 hours before testing. Normally, the rats are orally intubated and receive a volume of 5 ml/kg containing the appropriate concentration of test compound (based on mg/kg body weight). The vehicle control group of rats is also intubated by mouth. A positive control group of rats is also orally administered a control dose of 18 mg/kg of chlordiazepoxide. Random selection of the rats is utilized in dosing. The rats are returned to the cage for one hour. Sixty minutes after drug administration, the rat is quietly removed from its cage and the hind feet wiped with Signa electrode gel made by Parker Laboratories of Orange, New Jersey. When intraperitoneal (i.p) administration is used, the protocol is identical except that the drugs are administered in varying concentrations in a volume of 5 ml/kg 30 minutes prior to testing. Concentrations ranged from 0.4 to 50 mg/kg. The rat is placed on the floor in the chamber facing the licking tube. The animal is allowed 5 minutes to make 20 licking responses and receive the first shock (0.5 mA). If this response does not occur, the animal is removed and eliminated from the study. If 20 licking responses are made, the animal is permitted an additionnal 3 minutes during which time each 20 th lick is paired with a 0.5 mA shock. This period is automatically started, counted and terminated. The number of licks and shocks are recorded. The activity of the compound tested is evaluated by comparing the mean shocks of the group dosed with the test compound to both the mean shocks of the vehicule and positive control groups via a Students′ t-test. In general, an increase in the number of shocks received compared to the control is indicative of the anti-conflict or anti-anxiety activity of the compound. Representative compounds of the present invention typically show results in the SSD test indicative of the presence of anti-anxiety activity. The compounds of the present invention may also be used as biochemical tools. In general, no sign of overt toxicity has been observed with compounds of the invention at dosages at least several mutiples of their minimum effective doses in the SSD Test. According to a further feature of the invention there is provided a pharmaceutical composition which comprises a quinoline carboxamide derivative of the invention in association with a pharmacetuically acceptable diluent or carrier. The pharmaceutical composition may, for example, be in a form suitable for oral, rectal or parenteral administration, for which purposes it may be formulated by means known to the art into the form of, for example, tablets, capsules, aqueous or oily solutions or suspensions, emulsions, dispersible powders, suppositories or sterile injectable aqueous or oily solutions or suspensions. A preferred pharmaceutical composition of the invention is one suitable for oral administration in unit dosage form, for example a tablet or capsule which contains between 0.1 mg and 500 mg of the quinoline carboxamide derivative, or one suitable for intravenous, intramuscular or subcutaneous injection; for example, a sterile injectable containing between 0.1% and 10% w/w of the quinoline carboxamide derivative. The pharmaceutical composition of the invention will normally be administered to mammals such as man for relief of anxiety and tension in the same manner as that employed for chlordiazepoxide, due allowance being made in terms of dose levels for the potency and duration of actions of the quinoline carboxamide derivative of the invention relative to chlordiazepoxide. Thus each individual, will receive an oral dose of between 0.5 mg and 500 mg, and preferably between 0.5 mg and 20 mg, of quinoline carboxamide derivative, or an intravenous, subcutaneous or intramuscular dose of between 0.5 mg and 100 mg, and preferably between 0.5 mg and 20 mg, of the quinoline carboxamide derivative, the composition being administered one to four times per day. The rectal dose will be approximately the same as the oral dose. The invention is illustrated but not limited by the following examples in which temperatures are in degrees Celsius, ambient temperature refers to 23±3. Chemical symbols have their usual meanings unless otherwise specified and the following abbreviations are used: ml (milliliter), g (gram), mg (milligram), m.p. (melting point), tlc (thin layer chromatography), R f (relative mobility in tlc), < (less than), > (greater than), atmospheric pressure (1.013x10⁵ Pascals/atm), hr. (hour), min. (minutes). Ra, Rb, Rc, Rd, and Re have the meanings defined above and have no other chemical significance unless otherwise noted. Unless otherwise stated, solvent ratios are by a volume/volume (v/v) basis. Example 1 a. 4-Amino-8-ethylquinoline-3-(N-propyl)carboxamide (Formula I, Ra=ethyl, Rb=propyl, Rc=Rd=hydrogen) A mixture of the quinoline carboxylic acid (1.1 g) described in Example 1f and thionyl chloride (0.73 ml) was heated to reflux in methylene chloride (15 ml). After heating 96 hr, the mixture was cooled in an ice bath and propylamine (4 ml) added quickly. After stirring for a few minutes the volatiles were removed in vacuo and the residue partitioned between ethyl acetate and water. The layers were separated and the aqueous layer was extracted with two more portions of ethyl acetate. The combined organic layer was dried over MgSO₄ and concentrated to a solid residue (600 mg). Recrystallization from methylene chloride/hexane afforded the title compound as fine white needles with a m.p. of 166-167.5. Analysis calculated for C₁₅H₁₉N₃O: C, 70.01; H, 7.44; N, 16.33 Found: C, 69.67; H, 7.44; N, 16.26 b. Diethyl (2-ethylphenyl)aminomethylenemalonate A mixture of 2-ethylaniline (11.7 g) and diethyl ethoxymethylene malonate (25.0 g) were warmed together at 80°C for one hour. After cooling, the mixture was poured with stirring into a mixture of 150 ml of ether and 75 ml of hexane. After cooling the solution in an ice bath colorless needles precipitated and were collected by filtration. There was obtained 26,7 g (95%) of the title product; tlc, R f =0.73, silica gel, ethyl acetate:hexane (1:1). c. Ethyl 8-ethyl-4-hydroxyquinoline-3-carboxylate (Formula IV, Ra=ethyl, A¹=-CO₂CH₂CH₃) A solution of the product described in Example 1b (26.7 g) in diphenyl ether (about 50 ml) was heated with stirring to 250° for 1.5 hr. After cooling to ambient temperature, ether (50 ml) was added and the precipitate filtered. The supernatant was concentrated to remove ether then reheated to 250°C for an additional 1.5 hr. After cooling to ambient temperature and adding ether, an additional crop of material was obtained. There was obtained a total of 20.0 g (85%) of the crude product with a m.p. of 220-230°C. d. Ethyl 4-chloro-8-ethylquinoline-3-carboxylate The hydroxyquinoline (8.0 g) described in Example ic was combined with phosphoryl chloride (15 ml) followed by warming to 75°C for 30 min. After cooling the residue was taken up in ethyl acetate (100 ml) and water (50 ml) was carefully added. Saturated aqueous sodium bicarbonate (45 ml) was added and the layers separated. The ethyl acetate layer was dried over MgSO₄ and concentrated to leave a viscous oil (8.13 g, 94%); tlc, R f =0.72, silica gel, ethyl acetate : hexane (1:1). e. Ethyl 4-amino-8-ethylquinoline-3-carboxylate (Formula IIa, Ra=ethyl, Rd=H, A¹=-CO₂CH₂CH₃) The quinoline ester (8.13 g) from Example 1d was placed in a stainless steel pressure vessel and ethanol (50 ml), which had been cooled in an ice bath and saturated with ammonia, was added. The pressure vessel was sealed and heated to 100°C for 9 hr. After cooling and filtering the mixture 6.4 g (85%) of the aminoquinoline was obtained as yellow needles with a m.p. of 188-191°C; tlc, R f =0.54, silica gel, ethyl acetate:hexane (1:1). f. 4-Amino-8-ethyl-3-quinoline carboxylic acid (Formula II, Ra=ethyl, Rd=H, A=-COOH) A mixture of the aminoquinoline (24 g) described in Example 1e and methanol (100 ml) was treated with 10N KOH (40 ml, aqueous). The resulting mixture was stirred for 12 hr. at ambient temperature. The mixture was cooled and partitioned between water (100 ml) and ether (50 ml). The ether layer was separated and the aqueous phase acidified with glacial acetic acid. The precipitate was filtered off and dried overnight under high vacuum to afford the acid (14.0 g, 76%) as a white powder with a m.p: >250°C. Example 2 4-Amino-8-propylquinoline-3-(N-propyl)carboxamide (Formula I, Ra=propyl, Rb=propyl, Rc=Rd=hydrogen) The processes described in Examples 1b-1f were repeated using 2-propylaniline instead of 2-ethylaniline in Example 1b to afford the quinoline carboxylic acid of Formula II (Ra=propyl, Rd=hydrogen, A=-COOH). Then a procedure similar to that described for Example 1a was followed except the quinoline carboxylic acid (1.1 g) was reacted with thionyl chloride (1.2 ml) in methylene chloride (100 ml). After heating for 96 hr. and quenching with propylamine (10 ml) at 0°C, the crude product was isolated as described in Example 1a. Recrystallization from chloroform/hexane gave the title compound as a white solid (0.83 g, 64%) with a m.p. of 118-119°; tlc, R f =0.32, silica gel, ethyl acetate:hexane (2:3). Analysis calculated for C₁₆H₂₁N₃O: C, 70.82; H, 7.80; N, 15.49 Found: C, 70.93; H, 8.07; N, 15.39 Example 3 4-Amino-8-propylquinoline-3-[N-(2-propenyl)]carboxamide (Formula I, Ra=propyl, Rb=2-propenyl, Rc=Rd=hydrogen) The procedure described in Example 2 down to the formation of the quinoline carboxylic acid (1.8 g) was repeated using the methods described in Examples 1b-1f. The quinoline carboxylic acid (1.8 g) was reacted as described in Example 2 (1.8 g) with thionyl chloride (1.2 ml) in methylene chloride (100 ml). After heating for 96 hr. the mixture was cooled to 0° and quenched with 2-propenylamine (10 ml). The crude product was isolated as described in Example 1a. The residue was recrystallized from chloroform/hexane to give a white solid (0.68 g, 32%) with a m.p. of 125-126°; tlc, R f =0.38, silica gel, ethyl acetate:hexane (2:3). Analysis calculated for C₁₆H₁₉N₃O: C, 71.35; H, 7.11; N, 15.60 Found: C, 71.00; H, 7.30; N, 15.46 Example 4 4-Amino-8-pentylquinoline-3-(N-propyl)carboxamide (Formula I, Ra=pentyl, Rb=propyl, Rc=Rd=hydrogen) The processes described in Examples 1b-1f were repeated using 2-pentylaniline (3.38 g) in Example 1b instead of 2-ethylaniline. A quinoline carboxylic acid of the formula II (Ra=pentyl, Rd=H, A=COOH) was obtained. To a mixture of this quinoline carboxylic acid (0.50 g) and chloroform (10 ml) was added thionyl chloride (0.42 ml). After stirring for 2.5 hr. at ambient temperature the mixture was cooled to 0° and propylamine (1.90 ml) was added all at once. The clear, yellow solution was stirred for 15 min and then poured, with stirring, into water. Additional chloroform was added and the layers were separated. The chloroform layer was washed with water, then dried over MgSO₄. After concentrating the material there was obtained 0.48 g of a yellow solid. The crude solid was dissolved in methylene chloride and chromatographed over silica gel using methanol:methylene chloride (1:24) as the eluent. The resulting solid was recrystallized from petroleum ether to give the title compound (0.34 g, 59%) as a white solid, with a m.p. of 138.5-140.5°; tlc, R f =0.70, silica gel, methanol:methylene chloride (2:23). Analysis calculated for C₁₈H₂₅N₃O: C, 72.20; H, 8.42; N, 14.03 Found: C, 71.99; H, 8.31; N, 13.99 Example 5 4-Amino-8-pentylquinoline-3-[N-(2-propenyl)]carboxamide (Formula I, Ra=pentyl, Rb=2-propenyl, Rc=Rd=hydrogen) The procedures of Examples 1b-1f were followed to obtain the quinoline carboxylic acid except that 2-pentylaniline (3.38 g) was used instead of 2-ethylaniline and was reacted with 4.48 g of diethylethoxymethylenemalonate to obtain a compound of formula II (3.52 g, 66%) (Ra=pentyl, Rd=H, A=-COOH). Then, using this quinoline carboxylic acid (0.95 g), a procedure similar to that described in Example 4 was followed except that the quinoline carboxylic acid was reacted with thionyl chloride (0.40 ml) in chloroform (9.5 ml), followed by reaction with 2-propenylamine (1.48 ml). After work-up of the reaction mixture as described in Example 4 the crude product was chromatographed over silica gel using methanol/methylene chloride (1:24) as the eluent. There was obtained a yellow solid (0.44 g, 40%). Recrystallization from petroleum ether gave a white solid, m.p. 127.5-128.5°; tlc, R f =0.32, silica gel, methanol:methylene chloride (1:24). Analysis calculated for C₁₈H₂₃N₃O: C, 72.69; H, 7.79; N, 14.13 Found: C, 72.69; H, 7.86; N, 14.08 Example 6 4-Amino-8-butylquinoline-3-(N-propyl)carboxamide (Formula I, Ra=butyl, Rb=propyl, Rc=Rd=hydrogen) The processes described in Examples 1b-1e were repeated using 2-butylaniline (2.26 g) in Example 1b instead of 2-ethylaniline. A quinoline ester of formula IIa (Ra=butyl, Rd=H, A¹=-COOCH₂CH₃) was obtained. Then to a 0°C suspension of propylamine hydrochloride (0.96 g) in toluene was added dropwise a 2.3 M solution of trimethylaluminum (4.35 ml) in hexane. Following the addition the mixture was warmed to ambient temperature and stirred for 1 hr during which time the suspended amine hydrochloride was consumed. The resulting solution was cooled to 0° and the quinoline ester (1.09 g) was added quickly under a blanket of nitrogen. The mixture was heated to 100° and stirred for 2 hr. After cooling, the mixture was carefully quenched with aqueous hydrochloric acid and the mixture extracted with both ethyl acetate and CH₂Cl₂. The combined extracts were dried (MgSO₄) and concentrated to leave a crude solid. Purification by column chromatography over silica gel eluting with ethyl acetate/hexane (2:3) afforded a white solid. Recrystallization from CH₂Cl₂2/hexane gave 0.90 g (79%) of the title compound, m.p. 128-129°. Analysis calculated for C₁₇H₂₃N₃O: C, 71.55; H, 8.12; N, 14.72 Found: C, 71.23; H, 7.89; N, 14.69 Example 7 4-Amino-8-butylquinoline-3-[N-(2-propenyl)]carboxamide (Formula I, Ra=butyl, Rb=2-propenyl, Rc=Rd=hydrogen) A procedure similar to that described in Example 6 was followed except that 1.1 g of the quinoline ester was used, 4:3 ml of trimethylaluminum was used, and 2-propenylamine hydrochloride (0.98 g) was used in place of propylamine hydrochloride. After work-up the crude product was purified by chromatographing it over silica gel using ethyl acetate:hexane (3:7) as the eluent. The purified solid was recrystallized from methylene chloride/hexane to give fine, white crystals (0.63 g, 75%) with a m.p. of 126-127°; tlc, R f =0.35, silica gel, ethyl acetate:hexane (2:3). Analysis calculated for C₁₇H₂₁N₃O: C, 72.05; H, 7.47; N, 14.83 Found : C, 71.93; H, 7.50; N, 14.82 Examples 8-11 The process of Example 6 was repeated using the quinoline ester described therein (formula IIa, Ra=butyl, Rd=H, A¹=COOCH₂CH₃) and the appropriate amine hydrochloride of formula RbNH₃Cl with Rb having the value listed in Table I. Thus quinoline amides of formula I were obtained where Ra=butyl, Rc=Rd=H and Rb has the value listed in Table I for the particular example. These compounds and their physical data are listed in Table I. Example 12 a. 4-Amino-8-(3-pentynyl)quinoline-3-(N-propyl)carboxamide (Formula I, Ra=3-pentynyl, Rb=propyl, Rc=Rd=hydrogen) A procedure similar to that described in Example 6 was followed except the quinoline ester product from Example 12b (0.50 g) was reacted with the reagent prepared by reaction of propylamine hydrochloride (0.15 g) with 0.68 ml of a 2.35 M solution of trimethylaluminum in hexane. After recovery of the crude product as described in Example 6, a column was run over silica gel eluting with ethyl acetate:hexane (1:3) to give a white solid. Recrystallization from tert -butylmethyl ether/hexane gave the title compound (0.39 g, 75%) as a flocculent white solid, with a m.p. of 144-145°; tlc, R f =0.25: silica gel, ethyl acetate: hexane (1:3). Analysis calculated for C₁₈H₂₁N₃O: C, 73.19; H, 7.17; N, 14.22 Found: C, 73.77; H, 7.22; N, 14.26 b. Ethyl 4-Amino-8-(3-pentynyl)quinoline-3-carboxylate (Formula IIa, Ra=3-pentynyl, Rd=H, A¹= -COOCH₂CH₃) The processes described in Examples 1b-1e were repeated using 2-iodoaniline (3.54 g) instead of the 2-ethylaniline used in Example 1b; the 2-iodoaniline was reacted with 3.56 g of diethylethoxymethylenemalonate to prepare a quinoline ester of the formula IIb (2.35.g, 42%) where X=iodo, A¹=COOCH₂CH₃ and Rd=hydrogen. The Grignard reagent was prepared by slow addition of 1-bromo-3-pentyne (13 g) to a 0° suspension of magnesium turnings (2.42 g) in dry tetrahydrofuran (60 ml) under an argon atmosphere. After stirring for 2 hr. at 0°, the mixture was warmed to ambient temperature and stirred for 15 min. The unreacted magnesium was allowed to settle and the supernatant transferred via cannula to a second flask containing anhydrous zinc bromide dissolved in tetrahydrofuran (25 ml). A mechanical stirrer was used to efficiently mix the resulting thick white precipitate. Following the transfer, the mixture was stirred 15 min. at ambient temperature followed by the addition of catalytic dichloro[1,1′-bis(diphenylphosphino)ferrocene]palladian (II) (0.30 g). After stirring a few minutes, the quinoline ester obtained by the method of Example 1e (3.46 g) was added all at once under a back-flush of argon. The mixture was stirred at ambient temperature for 18 hr. followed by cooling in an ice bath and quenching slowly with saturated aqueous NH₄Cl. The mixture was partitioned between ethyl acetate and excess water and the layers separated. The organic layer was washed with brine, dried over Na₂SO₄ and concentrated. Purification by column chromatography over silica gel eluting with ethyl acetate:hexane (1:4) afforded the desired product as a yellow solid (1.42 g, 50%); tlc, R f =0.32: silica gel, ethyl acetate:hexane (1:3). Example 13 a. 4-Amino-8-(2-methylpropyl)quinoline-3-(N-propyl)carboxamide (Formula I, Ra=2-methylpropyl, Rb=propyl, Rc=Rd=hydrogen) A procedure similar to that described in Example 12 for the formation of the quinoline ester was followed using the quinoline described in Example 13b (0.5 g), commercially available 2-methylpropylmagnesium bromide (7.3 ml of a 2.0 m solution in ether) and 3.19 g of anhydrous zinc bromide. After completion of the reaction the crude product was isolated as described previously in Example 12a. Purification by column chromatography over silica gel using ethyl acetate:hexane as the eluent gave a white solid. Recrystallization from tert -butylmethyl ether/hexane gave a tan solid (0.17 g, 43%) with a m.p. of 138-140°; tlc, R f =0.36: silica gel, ethyl acetate: hexane (2:3). b. 4-Amino-8-iodoquinoline-3-(N-propyl)carboxamide (Formula III, X=iodo, Rb=propyl, Rc=Rd=hydrogen A procedure similar to that described in Example 6 for the formation of the quinoline ester was followed using the quinoline described in Example 12b (4.0 g), propylamine hydrochloride (2.3 g) and trimethylaluminum (10 ml of a 2.35 M solution in hexane). After isolation of the crude product as described in Example 6, the material was purified by column chromatography over silica gel using ethyl acetate:hexane (1:1) as the eluent. There was obtained 3.85 g (93%) of a cakey solid, m.p. 214-215°; R f =0.27: silica gel, ethyl acetate:hexane (2:3). Analysis calculated for C₁₇H₂₃N₃O: C, 71.55; H, 8.12; N, 14.72 Found: C, 71.22; H, 8.35; N, 14.46 Example 14 The following illustrates representative pharmaceutical dosage forms which may be used for the therapeutic or prophylactic administration of a compound of formula I or of a pharmaceutically acceptable salt thereof (hereinafter referred to as ′Compound A′): The lactose, sodid starch glycollate and polyvinylpyrrolidone are mixed in a planetary mixer and water added until a suitable mass for granulation is obtained. The mass obtained is granulated through a suitable size mesh and dried to obtain the optimum moisture content. The magnesium stearate is then added and the dry granulate is then passed through a further screen before final blending and compression to yield tablets each weighing 100 mg. The tablets are formulated as described in part a. to yield tablets each weighing 400 mg. The tablets are formulated as described in part a. to yield tablets each weighing 200 mg.

Claims: Claims for the following Contracting States : BE,CH,DE GB,IT,LI,NL,FR 1. A compound of formula I wherein Ra is selected from a group consisting of (2-6C)alkyl, (3-7C)cycloalkyl, (4-7C)cycloalkylalkyl, (3-6C)alkenyl, (3-6C) alkynyl, (6-10C)aryl, benzyl, (3-6C)haloalky having at least one halo group wherein the halo group(s) is selected from chloro and fluoro, (3-6C)hydroxyalkyl, (4-7C)hydroxycycloalkylalkyl, and 3-methyl-2-thienyl; Rb and Rc are independently selected from a group consisting of hydrogen, (2-6C)alkyl, (3-7C)cycloalkyl, (4-7C)cycloalkylalkyl, (3-6C)alkenyl, (3-6C)alkynyl, (2-6C)alkoxyalkyl, (2-6C)haloalkyl having at least one halo group (wherein the halo group(s) is selected from chloro and fluoro), and(3-6C)haloalkenyl having at least one halo group (wherein the halo group is selected from chloro and fluoro) and where the halo group(s) is on a carbon having a double bond; Rd is selected from a group consisting of hydrogen, (1-6C)alkyl and (1-6C)alkanoyl; and the pharmaceutically acceptable acid addition salts thereof. 2. A compound as claimed in claim 1 wherein Rd is selected from a group consisting of hydrogen and (2-4C)alkanoyl. 3. A compound as claimed in Claim 1 or 2 wherein Ra is selected from a group consisting of ethyl, propyl, butyl, pentyl, 3 -pentynyl and 2 -methyl-propyl; Rb is selected from a group consisting of propyl, 2-propenyl,cyclopropylmethyl, 2,2,2-tri-fluoroethyl, 2-chloro-2-propenyl, and 3,3-dichloro-2-propenyl; Rc is hydrogen; and Rd is hydrogen. 4. A compound as claimed in any one of Claims 1-3 selected from 4-amino-8-butylquinoline-3-[N-(2-propenyl)]carboxamide and 4-amino-8-butylquinoline-3-[N-propyl]carboxamide and the pharmaceutically acceptable salts thereof. 5. A salt as claimed in Claim 1 wherein said salt is made with an acid forming a physiologically acceptable anion. 6. A process for the manufacture of a compound of formula I or a pharmaceutically acceptable salt thereof as claimed in any one of Claims 1-5 which is characterised by: (a) reaction of a compound of formula II where A is a carboxylic acid (-COOH), or an activated derivative thereof, with an amine of formula RbRcNH; (b) reaction of a compound of formula IIa where A¹ is an ester having 1-6 carbons, with an aluminium reagent of the formula Re₂AlNRbRc or ReAl(Cl)NRbRc wherein Re is methyl, ethyl, propyl, or isobutyl; (c) reaction of a composed of formula III where X is iodo or bromo, with an organometallic reagent of formula RaMX or Ra₂M, wherein M is magnesium or zinc, in the presence of a palladium catalyst; and (d) for a compound of formula I in which Rd is alkanoyl, acylation of a corresponding compound of formula I in which Rd is hydrogen; wherein Ra, Rb Rc and Rd have the definitions stated in anyone of Claims 1-5 unless indicated otherwise; and whereafter, when a pharmaceutically acceptable salt is required, reacting a basic form of a compound of formula I with an acid forming a physiologically acceptable anion. 7. A compound of formula II or formula IIa wherein Ra and Rd have any of the meanings defined in Claims 1-5 and A and A¹ have any of the meanings defined in Claim 5. 8. A pharmaceutical composition comprising a compound of any one of Claims 1-3, or a pharmaceutically acceptable salt thereof in association with a non-toxic pharmaceutically acceptable diluent or carrier. 9. A compound of formula I, or a pharmaceutically acceptable acid addition salt thereof, as claimed in any one of claims 1 to 5, for use as an anxiolytic agent. 10. The use of a compound of formula I, or a pharmaceutically acceptable acid addition salt thereof, as claimed in any one of claims 1 to 5 for the manufacture of a medicament for treating anxiety in man. Claims for the following Contracting States : Es 1. A process for producing a compound of formula I wherein Ra is selected from a group consisting of (2-6C) alkyl,(3-7C)cycloalkyl, (4-7C)cycloalkylalkyl, (3-6C)alkenyl, (3-6C) alkynyl, (6-10C)aryl, benzyl, (3-6C)haloalkyl having at least one halo group wherein the halo group(s) is selected from chloro and fluoro, (3-6C)hydroxyalkyl, (4-7C)hydroxycycloalkylalkyl, and 3-methyl-2-thienyl; Rb and Rc are independently selected from a group consisting of hydrogen, (2-6C)alkyl, (3-7C)-cycloalkyl, (4-7C)cycloalkylalkyl, (3-6C)alkenyl, (3-6C)alkynyl, (2-6C)alkoxyalkyl, (2-6C)haloalkyl having at least one halo group (wherein the halo group(s) is selected from chloro and fluoro), and (3-6C)haloalkenyl having at least one halo group (wherein the halo group is selected from chloro and fluoro) and where the halo group(s) is on a carbon having a double bond; Rd is selected from a group consisting of hydrogen, (1-6C)alkyl and (1-6C)alkanoyl; and the pharmaceutically acceptable acid addition salts thereof, which method is selected from (a) reaction of a compound of formula II where A is a carboxylic acid(-COOH), or an activated derivative thereof, with an amine of formula RbRcNH; (b) reaction of a compound of formula IIa where A¹ is an ester having 1-6 carbons, with an aluminium reagent of the formula Re₂AlNRbRc or ReAl(Cl)NRbRc wherein Re is methyl, ethyl, propyl, or isobutyl; (c) reaction of a compound of formula III where X is iodo or bromo, with an organometallic reagent of formula RaMX or Ra₂M, wherein M is magnesium or zinc, in the presence of a palladium catalyst; and (d) for a compound of formula I in which Rd is alkanoyl, acylation of a corresponding compound of formula I in which Rd is hydrogen; wherein Ra, Rb Rc and Rd have the definitions stated above; and whereafter, when a pharmaceutically acceptable salt is required, reacting a basic form of a compound of formula I with an acid forming a physiologically acceptable anion. 2. A process as claimed in Claim 1 wherein Ra is selected from a group consisting of ethyl, propyl, butyl, pentyl, 3-pentynyl and 2-methyl-propyl; Rb is selected from a group consisting of propyl, 2-propenyl, cyclopropylmethyl, 2,2,2-tri-fluoroethyl, 2 chloro-2propenyl, and 3,3-dichloro-2-propenyl; Rc is hydrogen; and Rd is hydrogen. 3. A process as claimed in Claim 1 wherein the compound of formula I is selected from 4-amino-8-butylquinoline-3-[N-(2-propenyl)]carboxamide and 4-amino-8-butylquinoline-3-[N-propyl]carboxamide and the pharmaceutically acceptable salts thereof.

Inventors: WARAWA, EDWARD JOHN
Applicants: ICI AMERICAS INC.
Application year: 1987
Main classification: C07D215
IPC codes: C07D409, A61P25, C07D215, A61K31, C07B61, B01J23