DERIVATIVES OF CERVINOMYCIN ANTIBIOTICS AND PROCESS FOR THEIR PREPARATION

Abstract: Cervinomycin derivs. of formula (I) are new: where at least one of R1, R2 and R3 is acyl or alkyl and the rest are H; R4 is H or alkyl; B is a ring moiety of formula (II) or (III). Reaction of cervinomycin A2 (I, R1 = R4 = H, B is (II)) or cervinomycin A1 (I, R1 = R2 = R3 = R4 = H, B is (III)) with an alkylating or acylating agent.

Description: Technical Field The present invention relates to novel derivatives of cervinomycin based antibiotics and a process for producing the same. Background Technique Several compounds have been known as antibiotics having xanthone in their basic structure and they include: albofungin (= Kanchanomycin, BA-180265*, P-42-1**) as reported in Tetrahedron Letters , 18 , 1751 (1972), Antimicrob. Agent & Chemoth. , 767 (1962)*, and J. Antibiotics. , 26 , 65 (1973)**; lysolipin I as reported in Arch. Microbid. , 106 , 175 (1975) and Helv. Chim. Acta. , 60 , 178 (1977); antibiotic AM-5344-A₁ as disclosed in Japanese Patent Application (OPI) No. 9690/1983 (this antibiotic was later designated Cervinomycin A₁); and antibiotic AM-5344-A₂ as disclosed in Japanese Patent Application (OPI) No. 102897/1982 (this antibiotic was later designated Cervinomycin A₂). The present inventors investigated the solubility and toxicity of the cervinomycin compounds mentioned above and found that they were very poorly soluble in a variety of solvents, had strong toxicity and that they exhibited weak activity against Mycoplasmas and certain Gram-positive bacteria. Under these circumstances, it is necessary for the purposes of medical treatment of human and animal subjects to develop novel derivatives of cervinomycin that have enhanced solubility, improved antibacterial activity and reduced toxicity. Disclosure of the Invention Therefore, the present inventors analyzed the structures of Cervinomycin A₁ and A₂ and undertook synthesis of various derivatives thereof with a view to solving the aforementioned problems of these antibiotics. As a result, the present inventors found that novel cervinomycin derivatives of formula [1] noted below exhibit strong activity against Gram-positive and Gram-negative bacteria, as well as pathogenic microorganisms like mycoplasmas. The derivatives exhibit particularly high activity against mycoplasmas that are the causative organism of Mycoplasma pneumonia, and against a variety of anaerobic organisms. The present inventors also found that these derivatives had enhanced solubility in solvents and that yet their toxicity was markedly reduced as compared with cervinomycins and known related antibiotics having xanthone in their basic structure. The present invention has been accomplished on the basis of these findings. Accordingly, the present invention is to provide a compound represented by formula (1): wherein ring B represents at least one of R₁, R₂ and R₃ is an acetyl group and the others are a hydrogen atom; . The present invention also provides a process for producing such a compound. Compound [1] of the present invention can be produced by reacting Cervinomycin A₁ or A₂ with an acetylating agent in a solvent. According to this reaction, the hydroxyl group in Cervinomycin A₁ or A₂ having the chemical structure shown below is acetylated with an acetylating agent. wherein ring A represents is Cervinomycin A₁, and is Cervinomycin A₂. The acetylating agent may be acetic acid or reactive derivatives thereof. The term "reactive derivatives" means reactive derivatives of acetic acid that are generally used in esterifying a hydroxyl group, and examples of such derivatives include the acid halides such as acid chloride and acid bromide, as well as acid azide, acid anhydrid, mixed acid anhydrides, and active esters. Other acetylating agents known in the art may of course be used. If the acetic acid is used as such, condensing agents such as carbodiimides (e.g., N,N'-dicyclohexylcarbodiimide (DCC)) and N,N'-carbonylbisimidazole may be used in combination for achieving acetylation. The acetylating of the hydroxyl group in Cervinomycin A₁ or A₂ is carried out in a reaction solvent. A suitable reaction solvent is an organic solvent such as chloroform or pyridine. The reaction is generally performed in the presence of a catalyst selected from tertiary organic amines such as triethylamine, 4-dimethylaminopyridine, 4-piperidinopyridine and tribenzylamine. The reaction can be monitored by a suitable technique such as thin-layer chromatography on silica gel or high-performance liquid chromatography, whereby the operator may terminate the reaction after confirming that a maximum yield of the end compound (1) has been produced. As a result of the reaction, all of the hydroxyl groups in cervinomycin (2) are usually acetylated but if a cervinomycin having three hydroxyl groups is used as the starting material, compound (1) having only one or two hydroxyl groups acetylated may result depending upon the type of acetylating agent used, the presence or absence of a catalyst, the proportion of such agent or catalyst, or upon the duration of reaction time. The end compound (1) can be recovered from the reaction solution by first pouring it into water, then extracting compound (1) with a non-hydrophilic organic solvent such as ethyl acetate or chloroform. If desired, compound (1) may be further purified with a suitable isolation and purification technique such as column chromatography using a known adsorbent such as silica gel. Compound (1) of the present invention may be used as a pharmaceutical composition that contains it as an active ingredient and which can be administered by any method such as intravenous injection, intramuscular injection, peroral application, rectal application and aerosol application. This composition may be either solid or liquid and can be formulated by routine procedures in any of the dosage forms currently employed in pharmaceutical areas such as sugar-coated tablets, uncoated compressed tablets, gelatin capsules, granules, suppositories, injections, ointments and cream. In addition to the active ingredient, these pharmaceutical compositions may contain common excipients such as talc, gum arabic, lactose, starch, magnesium stearate, cocoa butter, animal and vegetable fats, paraffin derivatives and glycol, as well as a variety of humectants, dispersants, emulsifiers or preservatives. The dosage of the end compound (1) may be properly determined according to such factors as the severity of the disease to be treated, the age of patient, and the route of administration. Best Mode for Carrying Out the Invention Example 1 Preparation of triacetyl Cervinomycin A₁ Triacetyl Cervinomycin A₁: 500 mg of Cervinomycin A₁ was added to a mixture of 2 mℓ of acetic anhydride and 15 mℓ of pyridine. After addition of 1.2 mℓ of triethylamine, the mixture was stirred at room temperature for 4 days. The reaction mixture was poured into 300 mℓ of ice water, and the reaction product was extracted with 150 mℓ of ethyl acetate. The ethyl acetate layer was separated, washed three times with 100 mℓ portions of water, dried with anhydrous sodium sulfate, and concentrated under vacuum to obtain 630 mg of a brown powder. The powder was purified by silica gel (Kiesel gel G 60) column chromatography (eluting solvent: benzene/acetone (60:1 - 30:1)) to obtain a pale yellow powder of triacetyl Cervinomycin A₁ in an amount of 580 mg (yield: 96%). Melting point: 280 - 283°C Specific rotation: [α] 23 / D - 132° (c = 0.5 chloroform) UV spectrum: 259 (21,200), 299 (31,180), 355 (10,480), 371 (16,500), 414 (5,700) nm Molecular formula: C₃₅H₂₉NO₁₂ (from high-resolution mass spectrometry and elemental analysis) The powder was found to have the chemical structure shown above by analyses of IR, ¹H and ¹³C-NMR spectra. Example 2 Preparation of monoacetyl Cervinomycin A₂ Monoacetyl Cervinomycin A₂: A mixture of 1.6 mℓ of acetic anhydride and 12 mℓ of pyridine was added to 500 mg of Cervinomycin A₂. After addition of 1.0 mℓ of triethylamine, the mixture was stirred at room temperature for 3 days. The reaction mixture was poured into 250 mℓ of ice water and the reaction product was extracted with 150 mℓ of ethyl acetate. The ethyl acetate layer was separated, washed three times with 100 mℓ portions of water, dried with anhydrous sodium sulfate, and concentrated under vacuum to obtain 610 mg of a brown powder. The powder was purified by silica gel (Kiesel gel 60) column chromatography (eluting solvent: benzene/acetone (60:1 - 30:1)) to obtain a pale orange powder of monoacetyl Cervinomycin A₂ in an amount of 510 mg (yield: 94%). Melting point: 295 - 300°C Specific rotation: [α] 20 / D - 497° (c = 0.5 chloroform) UV spectrum: 248 (30,950), 274 (21,400), 307 (25,500), 374 (9,100) nm Molecular formula: C₃₁H₂₃NO₁₀ (from high-resolution mass spectrometry) The powder was found to have the chemical structure shown above by analyses of IR, ¹H and ¹³C-NMR spectra. Example 3 O -methyl Cervinomycin A₂ C -methyl Cervinomycin A₂ C , O -dimethyl Cervinomycin A₂ To 500 mg of Cervinomycin A₂, 150 mℓ of a mixture of chloroform and a small amount of methanol was added. After successive addition of 600 mg silver oxide and a large excess of methyl iodide, the mixture was stirred at 30°C for 3 days. The resulting reaction mixture was filtered to remove the silver oxide and the filtrate was concentrated by evaporation to dryness so as to obtain 570 mg of a brown powder. This powder was purified by silica gel (Kiesel gel 60) column chromatography (eluting solvent: chloroform/methanol/1% aqueous ammonia (60:1:1 - 40:1:1)). The purified product contained three methylated compounds, which were purified by silica gel thin layer chromatography (developing solvent: chloroform/methanol/1% aqueous ammonia (30.1:1)) to obtain 170 mg of O -methyl Cervinomycin A₂, 20 mg of C -methyl Cervinomycin A₂, and 120 mg of C , O -dimethyl Cervinomycin A₂, each in an orange powder form. O -methyl Cervinomycin A₂: Melting point: >300°C (with decomposition) Specific rotation: [α] 20 / D - 499° (c = 0.5, chloroform) Molecular formula: C₃₀H₂₃NO₉ (from high-resolution mass spectrometry) C -methyl Cervinomycin A₂: Melting point: >260°C (with decomposition) Specific rotation: [α] 20 / D - 460° (c = 0.3, chloroform) Example 3 Antibacterial activity Table 1 shows the minimum inhibitory concentrations (MIC) of the compounds shown in Table 1 on different microorganisms that were inoculated in an amount of 1 × 10⁶ cells/mℓ and cultivated on heart infusion agar (Difco) at 37°C for 20 hours, on GAM agar (Nissui) at 37°C for 20 hours, or on PPLO agar (Eiken) at 37°C for 7 days. Example 4 Acute toxicity Compounds (1) of the present invention were subjected to an acute toxicity test on ddY mice by oral administration. Example 5 Solubility Compounds (1) of the present invention had the following solubilities in different solvents. Industrial Applicability As the above data show, compounds (1) of the present invention are novel antibiotic derivatives. In comparison with the starting cervinomycin (2), the compounds show an extremely high activity against Gram-positive bacteria such as Staphylococcus, Bacillus, Micrococcus and Clostridium, Gram-negative bacteria such as Bacteroides , and against Mycoplasma, and they yet have low toxicity and are extremely soluble in various solvents. The antibacterial activity of compounds (1) is comparable to or greater than that of the known antibiotic clindamycin. Therefore, the compounds are useful as therapeutical drugs for infectious diseases that are caused by pathogenic organisms such as the above-mentioned Gram-positive bacteria, Gram-negative bacteria and Mycoplasma and which are exemplified by septicemia caused by infection with Staphylococcus , Clostridium and Bacteroides , and bronchopneumonia caused by infection with Staphylococcus and Mycoplasma .

Claims: A compound represented by formula: wherein ring B represents at least one of R₁, R₂ and R₃ represents an acetylgroup and the others represents a hydrogen atom; A process for producing a compound represented by formula wherein ring B represents at least one of R₁, R₂ and R₃ represents an acetylgroup and the others represent a hydrogen atom; comprising the step of reacting a compound represented by formula wherein ring A represents with an acetylating agent in a solvent.

Inventors: IWAI YUZURU - - KARABE -CHOME, NAKAGAWA AKIRA SUMIYOSHI JUTAK, OMURA SATOSHI - SETA -CHOME, IWAI, YUZURU 9-303, 2-1 KARABE 2-CHOME, NAKAGAWA, AKIRA SUMIYOSHI JUTAKU 4-206, OMURA, SATOSHI 12-7, SETA 5-CHOME
Applicants: KITASATO INST, THE KITASATO INSTITUTE
Application year: 1987
Main classification: C07D498
IPC codes: A61K31, C07D498, A61P31