COMPLEX OF PRODUCTS FOR THE RAPID AND NOT-INVASIVE TREATMENT OF BILIARY CALCULOSIS

Abstract: The present invention relates to a complex of products useful for the not-invasive and rapid treatment of the biliary calculosis of cholesterolic nature. It is based upon the coupling of a special apparatus generating medium-energy or medium-low-energy ultrasounds with pharmaceutical compositions based on biliary acids, capable of de-saturating bile super-saturated with cholesterol

Description: Biliary calculi (cholelithiasis) is a widely diffused pathologic condition which arises from a metabolic error at liver level, an error which essentially results in the production of lithogenous bile, i.e., a bile supersaturated in cholesterol; from this situation, the process originates, which leads, also with the cooperation of other factors, to the precipitation of cholesterol crystals and to the formation of calculi. The true epidemiological dimension of cholelithiasis has long been underestimated, however recent data indicates that at least 15% of the adult population is affected by such a disease, even if in most cases it is disregarded, in as much as it can develop in a completely asymptomatic way. The management of biliary calculi was, until approximately ten years ago, an exclusively surgical competence; the advent in therapy of the biliary acids capable of de-saturating the lithogenous bile opened new therapeutical possibilities, in that they made possible the dissolution in a medical way, even if by very long-lasting treatments (of from 6 months up to 2 years), of the already formed biliary calculi. The main impediment to the medical treatment by such biliary acids has anyway been the excessively long duration of the treatment, even when it has been suitably used in the indicated cases; said long duration causes frequently neglect by the patient of the prescription and also, in the long term, the possible arising, over time, of situations hindering the same dissolution of the calculus, such as, e.g., the deposition of calcareous concretions on calculus surface, such as to delay or prevent the dissolving thereof. More recent attempts to obtain an ultra-rapid dissolving of the calculi (in a few minutes or hours) were carried out by adopting means of invasive type: the local perfusion, by probes or catheters, of chemical solvents of ether type, the application of shock waves, and so forth. These are approaches which are still now at an experimental level, and which shall anyway remain limited to highly specialized Centres. For the solution of the problem, the present invention proposes a set of products for the rapid, non-invasive treatment of the biliary calculi, characterized in that it comprises a pharmaceutical composition containing, as its active principle, a litholytic biliary acid active in desaturating bile supersaturated in cholesterol, and a device generating medium-power or medium-low-power ultrasounds. These are a set of products which propose a treatment for the dissolution of the biliary calculi, having essential characteristics of non-invasiveness and of rapid achieving of the dissolution, by "rapidity" the reduction down to a few months (from 1 to 3 months) of the many months normally required for the medical treatment with the above mentioned litholytic biliary acids being understood. The set of products of the present invention is therefore constituted by: pharmaceutical compositions with biliary acids of the type definable as litholytic biliary acids active in desaturating, by different mechanisms, bile supersaturated in cholesterol; examples thereof are represented by chenodeoxycholic acid and ursodeoxycholic acid, but without limitations in this regard, in that more recently other biliary acids have shown to have analogous properties, and in a near future they will probably find similar clinic applications; special apparatuses, capable of producing low-energy and/or medium-energy ultrasounds, and of focusing their energy on the seats containing the calculary concretions (calculi in cholecyst) without altering, not even to a minimum extent, the surrounding tissues. It is known that low-energy or very low-energy ultrasound apparatuses have been long used for diagnostic purposes, and constitute a very valuable aid in various specialistic fields (cardiology, obstetrics, gastroenterology, and so forth), as well as very high-energy apparatuses are used for "aimed" applications, capable of "hitting" very well circumscribed seats, to destroy particular tissues or formations. The present invention relates, on the contrary, to the use of medium-low-energy apparatuses, i.e., devices with powers comprised within the range of from 2-3 milliwatts/cm² of application surface area, applied by pulsed emissions each thousandth of a second, up to powers of the order of 2-4 watts/cm² of application surface area, applied by emissions pulsed up to one each thousandth of a second. Such apparatuses are purposely prearranged for modifying and accelerating the rate of dissolution of the calculus in the complex system represented by the solid crystal of cholesterol inside the phospholipids-biliary acids-cholesterol medium, wherein the concentration of cholesterol has been decreased to levels of absolute or relative undersaturation, thanks to the effect of the continuous oral administration of one of the above mentioned biliary acids; the special apparatus of the invention can be optionally, but not necessarily, equipped with a suitable and precise aiming system, which is capable of identifying the application focus, and of concentrating the application beam as efficaciously as possible. Within the range of energy power envisaged by the present invention, at least two types of devices can be defined, one of which operates at a lower energy level for longer applications, and/or repeated applications at an out-patient and private level, and the other, operating at a higher energy level, and with a possible incorporated aiming system, is destined to shorter applications, and/or applications more spaced in time, to be carried out at specialistic Centres. For the purpose of better understanding the characteristics and the advantages of the invention, hereunder some examples of practical embodiment are supplied. Example 1 A patient with three radiotransparent calculi of medium size, operatable cholecystitis, recognized by echography and subsequent cholecystography. A treatment with chenodeoxycholic acid at the dosage rate of 15 mg/kg daily is established; in as much as the treatment is carried out on a subject of 80 kg, 5 capsules of 250 mg are administered daily. After 1 week of treatment with CDCA, a first 1-minute-long application of medium-energy ultrasound apparatus is carried out at a specialized Centre. The treatment with CDCA is continued, and regular weekly applications, always 1 minute long, are carried out with the ultrasound device. After 2 weeks of treatment with CDCA, the daily dosis is slightly reduced (4 capsules daily) due to the occurrence of a minor diarrhoea, but, after a further week, it is possible to return back to the initial dosage rate without any particular problems. The echographic checks are carried out with a regular two-weekly frequency, to monitor the beginning of a possible dissolution of the calculi. After two months, an evident partial dissolution of the calculi, and after four months a complete dissolution thereof, is observed. Example 2 A patient with five radiotransparent calculi of medium and small sizes, operatable cholecystitis, diagnosed by echography and subsequent cholecystography. A treatment with ursodeoxycholic acid at the dosage rate of 8 mg/kg daily is established; in as much as the treatment is carried out on a subject of 75 kg, 2 capsules of 300 mg are administered daily. After 2 weeks of treatment with UDCA, a daily application, of a duration of approximately 3 minutes each, Of the medium-low-energy ultrasound apparatus is prescribed. The treatment with UDCA is continued, and regular daily applications, always approximately 3 minutes long, are carried with the medium-low-energy ultrasound apparatus. The echographic checks are carried out with a regular two-weekly frequency, to monitor the proceeding of the calculus dissolution process. After 1 month, an evident partial dissolution of the calculi, and after two months the complete dissolution thereof, is observed. In general, it can be observed that a particularly relevant aspect of the present invention is represented by the fact that such apparatuses, within the provided energy range, are not capable of causing, per se , the disintegration of the biliary calculi, and, furthermore, that the litholytic biliary acids which can be used for dissolution purposes, when used as such, cause the dissolution of the biliary calculi susceptible of medical treatment in percentages of the order of from 30 to 50% of cases, and anyway require at least 6-12 months of administration; the combination of litholytic biliary acid administration with the periodical applications of the ultra-sound apparatus according to the invention causes, on the contrary, the dissolution of biliary calculi in slightly larger percentages of cases, but, above all, in considerably shorter times (of from 1 to 3 months); therefore, a surprising "synergistic action" between the components of the set of the present invention is evident.

Claims: Set of products for the rapid and non-invasive treatment of biliary calculi of cholesterolic nature, characterized in that it comprises a pharmaceutical composition containing, as its active principle, a litholytic biliary acid active in de-saturating the bile super-saturated in cholesterol, and a low-power or medium-power ultrasound generating apparatus. Set of products according to claim 1, characterized in that said ultrasounds have a power comprised within the range of from 2 milliwatts/cm² of application surface area, to 4 watts/cm² of application surface area, said power being applied by emissions pulsated every thousandth of second. Set according to claim 1, characterized in that said apparatus is equipped with an aiming device to define the focus of application of said ultrasounds. Therapeutical set for the rapid and non-invasive treatment of the biliary calculi of cholesterolic nature, characterized in that it comprises a litholytic biliary acid active in de-saturating the bile super-saturated in cholesterol, and a source of low-energy or medium-energy ultrasounds. Set according to the preceding claims, characterized in that said litholytic biliary acid and said ultrasounds must be periodically applied, at successive times, after each other. Set according to the preceding claims, characterized in that said litholytic biliary acid and said ultrasounds must be periodically applied according to a prearranged sequence. Set according to the preceding claims, characterized in that said litholytic biliary acid and said ultrasounds must be periodically applied, simultaneously to each other. Use of a litholytic biliary acid active in de-saturating the bile super-saturated in cholesterol for the preparation of a pharmaceutical composition to be used together with medium-energy or low-energy ultrasounds, for the rapid and non-invasive treatment of biliary calculi of cholesterolic nature.

Inventors: FRIGERIO, GIULIANO, RODA, ALDO, RODA, ENRICO
Applicants: GIULIANI S.P.A.
Application year: 1987
Main classification: A61K31
IPC codes: A61K31, A61B17, A61K41