MITOMYCIN DERIVATIVES

Abstract: Mitomycin derivatives of formula (I) are new. In (I) X1 and X2 are H or lower alkyl; Y and Z are H or methyl (but when Z is H then Y must be Me); R1 and R2 are carbamoyloxymethyl and H (one of each); n is 1-4; X is R3NH, R3O, R3S, R4CO, NaOSO2-, or a group of formula (II); R3 is an organic carboxylic acid residue, lower alkanoyl or halo-lower alkanoyl (e.g. an alpha aminoacid, orotic acid, isoorotic acid, nicotinic acid, isonicotinic acid, thiophenecarboxylic acid, furoic acid, vitamin A acid, or satd. or unsatd. mono or dibasic fatty acid residue+; R4 is an organic alcohol or amine group (such as beta-oestradiol, beta-oestradiol 3-benzoate, retinol, cortisone, corticosterone, ammonia, an alpha amino-acid, primary or secondary alkylamine, isoproterenol, epinephrine or norepinephrine); R5 is H, ribofuranosyl or 2-deoxyribofuranosyl. When X is (II), then CX1X2 is CH2.

Description: TECHNICAL FIELD : The present invention relates to mitomycin derivatives having anti-tumour activity and pharmaceutical compositions containing the same. BACKGROUND ART : Mitomycins have excellent anti-tumour activity, whilst they are liable to exhibit certain undesired side effects such as the decrease of leucocytes. Thus, attempts have been made to provide various mitomycin derivatives to increase anti-tumour activity and/or to decrease toxicity. The known mitomycin derivatives include mitomycin C derivatives and porfiromycin derivatives, which contain a substituted 7-amino group, disclosed, for example, in U.S. Patent 4268676: Japanese laid open Patent Applications 92288/81 and 188590/82: Journal of Medicinal Chemistry, 24 , 975-981 (1981): ibid., 26 , 16-20 (1983) and ibid., 26 , 1453- 1457 (1983)]. These prior art literatures disclose that mitomycin derivatives containing a substituted amino group at the 7th position exhibit anti-tumour activity in the living body. Among various mitomycin derivatives containing a substituted 7-amino group, those having a 7-amino group substituted with a sulphur atom are exemplified by the mitomycin derivatives containing at the 7th position 2-thiazolamino group, 2-thienylmethylamino group and (4-sulfonamidophenyl)methylamino group (disclosed in Japanese laid open Patent Application 92288/81), 2-mercaptoethylamino group, 2-ethylthioethylamino group, thiomorpholino group, thiazolidinyl group, 4-mercaptoanilino group, 2-(4-methylthiazolyl)amino group, 2-(5-methyl-1,3,4-thiadiazolyl)amino group and 4-(2,1,3-benzothiadiazolyl)amino group (disclosed in Japanese laid open Patent Application 188590/82). Examples of the known mitomycin derivatives containing at the 7th position a substituent of the formula:- RSS(CH₂)₂NH-, include those, of which R is an an alkyl or substituted alkyl such as 7-N-propyldithioethylmitomycin C, 7-N-methoxycarbonylmethyldithioethylmitomycin C and 7-N-[2-(2-hydroxyethyldithio)ethyl]mitomycin C (disclosed, for example, in EP 0116208A1 and Japanese laid open Patent Application 175493/84). Those, of which R contains an aromatic ring having a substituent, are exemplified by 7-N-[2-(4-acetamidophenyldithio)ethyl]-mitomycin C (disclosed in EP 0116208A1 and Japanese laid open Patent Application 175493/84) and 7-N-[2-(4-fluorophenyldithio)ethyl]mitomycin C (disclosed in EP 0163550A2 and Japanese laid open Patent Application 255789/75). Those, of which R contains a structural component of an amino acid having a thiol group or a peptide having the foregoing amino acid are exemplified by 7-N-[2-[(L-cystein-S-yl)thio]ethyl]mitomycin D and 7-N-[2-[(glycino-L-cystein-S-yl)thio]ethyl]mitomycin C, etc. (disclosed in EP 0163550A2 and Japanese laid open Patent Application 255789/85 etc.) Mitomycin derivatives containing at the 7th position a substituent of the formula:- -NHCH₂CH₂SSCH₂CH₂NH-, are exemplified by 7-N, 7'-N'-dithiodiethylenedimitomycin C, 7-N, 7'-N'-dithiodiethylenedimitomycin D, 7-N-[2-(2-aminoethyl)dithioethyl]mitomycin C, and 7-N-[2-(2-aminoethyl)-dithioethyl]mitomycin D (disclosed in EP 0116208A1, Japanese laid open Patent Applications 104386/84 and 175493/84) etc. Derivatives of mitomycin C containing R⁹-SS-alk₂-NH at the 7th position, are exemplified by the known compounds containing at the 7th position in which R is exemplified by CH₃-, and (disclosed in Japanese laid open Patent Application 205382/84). However, in the executional examples, only the derivatives of mitomycin C and 1a-acetylmitomycin C, both containing at the 7th position are disclosed, and other compounds and their physicochemical characteristics and anti-tumour effects are not disclosed An object of the present invention is to provide mitomycin derivatives containing a substituted amino group at the 7th position having higher anti-tumour activity lower side effects and/or higher solubility which is desirable in pharmaceutical preparation and anti-tumour compositions containing the same. DISCLOSURE OF THE INVENTION : The present invention provides mitomycin derivatives represented by the following formula (I):- wherein X' and X'' each independently represents hydrogen or lower alkyl; Y and Z each independently represents hydrogen or methyl; provided that when Z is hydrogen, Y is methyl; one of R₁ and R₂ represents carbamoyloxymethyl and the other represents hydrogen; n represents an integer of from 1 to 4; and X is R₃NH, R₃O or R₃S wherein R₃ is the residue of an α-amino acid from which OH of the carboxyl group has been removed, provided that when said α-amino acid contains a second carboxyl group, the second carboxyl group may be protected by C₁₋₄ alkyl or may form a salt with an alkali metal, ammonium or organic amine. Optionally, the amino group of said α-amino acid may be protected by C₁₋₄ alkanoyl or may form a salt with an inorganic or organic acid. In this specification, compounds represented by the formula (I) are designated as Compounds I. Compounds of other formulae are also designated similarly. With regard to the definition of Compounds I, C₁₋₄ alkyl are exemplified by, for example, methyl, ethyl, n-propyl and t-butyl. Examples of suitable α-amino acids include alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine, of which absolute configuration may be in L, D or DL form. Examples of suitable C₁₋₄ alkanoyl include for example, formyl, acetyl, propanoyl, n-butanoyl and t-butanoyl. Suitable alkali metals are exemplified by sodium and potassium. Suitable organic amines which may be used to form salts include, for example, monoethanolamine, N-methylglucamine and triethanolamine. Suitable inorganic acids which may be used to form salts include, for example, carbonic acid, sodium bisulfate, potassium dihydrogen phosphate and sodium dihydrogen phosphate. Suitable organic acids are exemplified by ascorbic acid, citric acid, succinic acid, acetic acid, tartaric acid and lactic acid. It has been found that, in the formula (I), wherein Z is hydrogen, it is possible by the following rearrangement (equilibrium) reaction to convert Compounds I into albomitomycin-type Compounds (I') which are also new compounds:- (wherein X, X', X'', R₁, R₂ , Y and n are as hereinbefore defined). The existence of albomitomycin-type and mitomycin-type mitomycin A and mitomycin C was previously known (Abstracts, the 27th Symposium on the Chemistry of Natural Products. pp. 672-679 1985, Hiroshima). In the case where the compound of Example I described hereinafter was equilibrated by allowing to stand in an aqueous solution (5 mg/ml) at a temperature of 25 °C for 24 hours, the molar ratio of the albomitomycin-type substance was 8,5 %. It has been found that the albomitomycin-type substances of the present invention are gradually converted into mitomycin-type substances, In the case of Compound I, for example, the compound had a half-time of 50 minutes and was equilibrated with mitomycin-type substance within a period of 5 hours by treating in an aqueous solution (5mg/ml) at a temperature of 26°C. Thus, it is believed, on the ground of the observation of mitomycin C, that the existence of albomitomycin-type substances may not harm the anti-tumour activity of the corresponding mitomycin-type substances. Moreover, it is possible to maintain a constant ratio between mitomycin-type substance and albomitomycin-type substance found in any compound of the present invention by selecting the reaction conditions appropriately. In summary, the existence of substances of two types in one compound of the present invention may not, in our view, give rise to deleterious effects with respect to clinical practice. The following Tables 1 and 2 indicate the names and structures of Compounds I according to the present invention. No. Compound 1 7-N-[2-[[2-(N⁵-L-glutamino)ethyl]dithio]ethyl]-mitomycin C 2 7-N-[2-[[2-(N⁵-L-glutamino)ethyl]dithio]ethyl]-mitomycin D 3 7-N-[2-[[2-(N⁵-D-glutamino)ethyl]dithio]ethyl]-mitomycin C 4 7-N-[2-[[3-(N⁵-L-glutamino)propyl]dithio]ethyl]-mitomycin C 5 7-N-[2-[[4-(N⁵-L-glutamino)butyl]dithio]ethyl]-mitomycin C 6 7-N-[2-[[2-(N⁴-L-asparagino)ethyl]dithio]ethyl]-mitomycin C 7 7-N-[2-[[2-(N²-L-asparagino)ethyl]dithio]ethyl]-mitomycin C 8 7-N-[2-[[2-(L-pyroglutamylamino)ethyl]dithio]-ethyl]mitomycin C 9 7-N-[2-[[2-(N²-L-glutamino)ethyl]dithio]ethyl]-mitomycin C 10 7-N-[2-[[2-(N⁴-L-asparagino)ethyl]dithio]ethyl]-mitomycin D 11 7-N-[2-[[2-(N⁵-D-glutamino)ethyl]dithio]ethyl]-mitomycin D 12 7-N-[2-[[2-(L-pyroglutamylamino) ethyl]dithio]-ethyl]mitomycin D 13 7-N-[2-[[2-(L-prolylamino)ethyl]dithio]ethyl]-mitomycin C Compounds I may be obtained by following three processes classified into Processes A, B and C. Process A Compound I may be prepared by the reaction of a compound of the formula (II):- (wherein R₁, R₂, Y and Z are as hereinbefore defined) with a compound of the formula (III):- X-(CX'X'') n SH (III) (wherein X, X', X'' and n are as hereinbefore defined) in an inert solvent. Compounds represented by the formula (II) are disclosed in EP 0116208A1 and Japanese laid open Patent Application 175493/84. Solvents which may be used for this reaction are exemplified by halogenated lower alkanes such as chloroform and dichloromethane: lower alkanols such as methanol, ethanol and isopropanol: tetrahydrofuran, ethylene glycol dimethylether, dioxane, acetonitrile. dimethylformamide, dimethyl sulfoxide and water, which may be used solely or in combination. The reaction temperature and the reaction time may vary, depending upon the types of Compounds III. However, usually it is possible to carry out the reaction at a temperature of from 0 to 30 °C for a period of several minutes to several hours. Compounds I may be purified, for example, by column chromatography, high performance liquid chromatography and TLC, for which various carriers may be used. The above-mentioned process is hereinafter referred to as Process A. Compounds III containing R₃NH as X, of which R₃ is the structural component of an α -amino acid, from which OH of the carboxyl group is excluded, may be obtained, for example, by Process (A-1) or (A-2) as follows:- Step (A-1) [wherein R₃' is the structural component of an α -amino acid, from which OH of the carboxyl group is excluded: the amino group is be protected by a group used conventionally for the protection in the synthesis of peptides: provided that when said α-amino acid has two carboxyl groups, one of the carboxyl groups is protected by a group conventionally used for the protection in the synthesis of peptides: optionally, the functional group of the side chain may also be protected: R₃'' is the structural component of an amino acid, from which OH of the carboxyl group is excluded (wherein all protective groups are removed): R₃''' is the structural component of an α -amino acid, from which OH of the carboxyl group is excluded: provided that said α -amino acid has an amino group and a carboxyl group, said amino group is protected by lower alkanoyl and/or said carboxyl group is protected by lower alkyl; X, X'' and n are as hereinbefore defined]. With regard to the definition of said R₃', suitable groups conventionally used for the protection of amino group are exemplified by benzyloxycarbonyl and t-butyloxycarbonyl, Suitable examples of the groups conventionally used for the protection of carboxyl group in the synthesis of peptides include benzyl and t-butyl groups. The above-mentioned process steps (A-1-1)-(A-1-5) are described in detail hereinafter. Step (A-1-1) Compounds III-1 and III-2 are condensed in conventional manner used for the synthesis of peptides such as, for example, by the active ester method and the acid chloride method. The former is exemplified in the following, with reference to N,N-dicyclohexylcarbodiimide (hereinafter referred to as DCC) additive method. To an inert solvent such as, for example, dichloromethane, chloroform, tetrahydrofuran, dioxane and dimethylformamide containing Compound III-1 may be added about an equimolar amount of a condensing agent such as, for example, DCC and an equimolar amount of N-hydroxysuccinimide or 1-hydroxybenzotriazole. The reaction may be carried out at a temperature of 0 °C to ambient for a period of from several hours to about 12 hours to obtain an active ester. Subsequently, to the reaction solution is added Compound III-2. The reaction may be effected at a temperature of 0 °C to ambient for a period of several tens of minutes to several hours to obtain Compound III-3. If desired, the active ester may be isolated, purified and used for the reaction with Compound III-2. The resultant Compound III-3 may be purified, for example, by recrystallization, chromatographic treatment using, for example, silica gel and alumina. N,N'-bis-[N-benzyloxycarbonyl-γ-(α-benzyl)-L-glutamyl]cystamine, which is one of the examples of Compound III-3 is disclosed by Laszlo Feuer et al in Japanese laid open Patent Application 4121/76 and DE 1518160. Step (A-1-2) The groups used for the protection of R₃' may be removed, for example, by the use of trifluoroacetic acid, trifluoromethanesulfonic acid or hydrogen fluoride in conventional manner. In the case of Compound III-3 where the amino group and the carboxyl group are respectively protected by benzyloxycarbonyl and benzyl, the protecting groups may may be removed by using 1 to 10 molar equivalents of trifluoromethanesulfonic acid in the presence of the same molar amount of anisole or thioanisole. The reaction may be effected at a temperature of from 0 °C to ambient for a period of several minutes to several hours in dichloromethane or trifluoromethane. In the case of Compound III-3 where the amino group and the carboxyl groups are respectively protected by t-butyloxycarbonyl and t-butyl, the protecting groups may be removed by the reaction using trifluoroacetic acid or trifluoroacetic acid-dichloromethane. The reaction may be effected in a similar manner to that described above. The resultant Compound III-4 may be purified, for example, by recrystallization, ion exchange method and various chromatographic treatments. Step (A-1-3) To obtain Compound III-5 from Compound III-4, the amino group of Compound III-4 may be protected by lower alkanoyl. Also, where carboxyl is present therein, the carboxyl may be protected as lower alkylester. The amino group may be protected by lower alkanoyl by the reaction of an equimolar amount of an alkanoyl halide or a corresponding acid anhydride in the presence of a slightly excessive amount of potassium hydroxide, sodium hydroxide or sodium bicarbonate in an aqueous solution of Compound III-4, which may be effected at a temperature of from 0°C to ambient for a period of from several tens of minutes to several hours. The carboxyl group may be converted into the corresponding lower alkyl ester, for example, by the thionyl chloride method, the azeotropic method or the ester exchange method. In the case of thionyl chloride method, a slightly excessive amount of thionyl chloride is added to said lower alkanol solution containing Compound III-4 to carry out the reaction at a temperature of from 0 °C to ambient for a period of from several hours to one day. The resultant Compound III-5 may be purified, for example, by recrystallization and ion-exchange method and chromatographic treatments including reversed phase high performance liquid chromatography using chemical-coupling type silica as carrier. Steps (A-1-4) and (A-1-5) Compounds III-4 and III-5 may be converted into Compound III, for example, by the reaction with an excessive amount of ethanethiol, 2-mercaptoethanol or dithiothreitol in a solvent such as, for example, water, methanol, ethanol, tetrahydrofuran, dioxane, acetonitrile and dimethylformamide at room temperature for a period of from several hours to several days. Compound III may be purified, for example, by recrystallization, ion-exchange method and chromatographic treatments such as, for example, reversed phase high performance liquid chromatography using chemical-coupling type porous silica as carrier. Step (A-2) [wherein R₃', R₃'', X' and X'' and n are as hereinbefore defined]. Step (A-2-1) Compounds III-1 and III-6 are subjected to a similar condensation to that described in Step (A-1-1) to obtain Compound III-7. Compounds III-6 are exemplified by 2-[(p-methoxybenzyl)thio]ethylamine. This compound and its preparation are disclosed in Armyanskii Khimicheskii Zhurnal, 1968, 21 (10), 858-863. Step (A-2-2) Compounds III-8 may be obtained, for example, by the use of trifluoromethanesulfonic acid/anisole, trifluoromethanesulfonic acid/thioanisole or hydrogen fluoride for removal of group used for the protection of the amino group of R₃', and/or the group used for the protection of the carboxyl group of R₃', if any carboxyl group is protected. The methoxybenzyl group linked with sulphur may also be treated similarly. Compounds III-8 may be prepared, for example, in the following manner. On the basis of Compound III-7, 1-10 molar equivalents of trifluoromethanesulfonic acid may be used. The reaction may be effected in the presence of the same molar amount of anisole or thioanisole in trifluoroacetic acid or trifluoroacetic acid/dichloromethane at a temperature of from 0°C to room temperature for a period of from several tens of minutes to several hours. It is also possible to remove the protecting groups by the reaction in a solution of hydrogen fluoride in the presence of 1/10 amount of anisole at a temperature of about 0°C for a period of 30-60 minutes. Compounds III-8 may be purified, for example, by recrystallization, ion-exchange method and chromatographic treatments including high performance liquid chromatography using chemical-coupling type porous silica as carrier. Process B is explained as follows:- Process B Compound 1 may be obtained by reacting a compound of the formula IV:- (wherein R₁, R₂, Y and Z are as hereinbefore defined) such as, for example, mitomycin A, mitomycin B [disclosed in Merck Index, 10th Edition, 6079 (1983)] or mitomycin J (disclosed in Japanese laid open Patent Application 45322/80) with a compound of the formula V:- X-(CX'X'') n -SS(CH₂)₂NH₂ (V) (wherein X, X', X'' and n are as hereinbefore defined) in an inert solvent. This process is hereinafter referred to as Process B. The solvents which may be used for the above-mentioned Process A may be used for this process. The reaction temperature and the reaction time may vary, depending upon the type and concentration of Compound V used. Usually it is sufficient to carry out the reaction at a temperature of from 0 to 80°C for a period of several tens of minutes to several hours. Compounds I may be purified, for example, by column chromatography, high performance liquid chromatography and TLC, for which various carriers may be used. Compounds V may be obtained by the following Step (B-1) or (B-2):- Step (B-1) [wherein R₃' is the structural component of an α-amino acid, from which OH of the carboxyl group is excluded: X', X'' and n are as hereinbefore defined]. Organic acids hereinbefore disclosed may be used for this procedure. Steps (B-1-1) and (B-1-2) may be carried out similarly to Steps (A-1-1) and Process A respectively. Compounds V-2 are exemplified by 2-pyridyldithioethylamine (dihydrochlorides) disclosed in Japanese laid open Patent Application 136261/80. Process C is described in detail as follows:- A compound of the formula (VI):- (wherein X', X'', R₁, R₂, Y, Z and n are as hereinbefore defined) is reacted with a compound of the formula (VII):- R₃OH or R₃SH (VII) (wherein R₃ is as hereinbefore defined) in the presence of triphenylphosphine and dialkyl azodicarboxylate in an inert solvent to obtain the desired Compound I. Compound (VI), of which X' and X'' each represents H and n is 2, is disclosed in Japanese laid open Patent Application 175493/84. Solvents which may be used for this reaction are exemplified by ether-type solvents such as diethylether and tetrahydrofuran: benzene, methylene chloride, hexamethyl-phosphorus triamide and other anhydrous solvents. Examples of dialkyl azodicarboxylates include diethyl azodicarboxylate and diisopropyl azodicarboxylate. For example, equimolar amounts of Compound VII. triphenylphosphine and dialkyl azodicarboxylate may be used on the basis of Compound VI, although it is possible, if desired, to use up to about 3 molar equivalents of each compound to improve the production yield. The reaction temperature and the reaction time may vary, depending upon the types of Compounds VI and VII. However, usually it is possible to carry out the reaction at a temperature of from -20 to 30°C for a period of from about several minutes to about one hour. Although the treatment after the reaction may vary, depending upon the type of Compound VII used, for example, it is possible to concentrate the reaction solution without any after-treatment. Alternatively, the reaction solution may be extracted with water-insoluble solvent such as, for example, chloroform, methylene chloride or ethyl acetate, and washed with water, sodium bicarbonate solution, followed by concentration. The concentrated material may be purified, for example, by column chromatography, TLC and recrystallization. Compounds I exhibit excellent anti-bacterial and anti-tumour activities and may be used as anti-bacterial and anti-tumour agents. In comparison with mitomycin C which is widely and clinically used as anti-tumour agent, Compounds I exhibit, in general, higher anti-tumour activity and lower bone marrow supression. That is, Compounds I have better C.I. (LD₅₀/ED₅₀) and wider tolerance of dosage, greater WBC₄₀₀₀ (minimum dosage required to reduce the number of peripheral leucocytes to 4000/mm³) and lower bone marrow supression in comparison with mitomycin C. Among water-soluble Compounds I, of which R₃ is the structural component of an amino acid, from which OH of the carboxyl group is excluded, those containing the structural component of glutamic acid or the structural component of aspartic acid exhibit better anti-tumour activity. Among such compounds, those, of which n is an integer of from 2 to 4, exhibit, in general, high water-solubility and are suitable for the preparation of injection agents. Especially good results may be obtained by Compound I, of which X is the structural component of glutamic acid substituted at the γ position: n is 2: X' is H; X'' is H; R₁ is CH₂OCONH₂; R₂=H; Y=CH₃ and Z is H. The C.I. value (LD₅₀/ED₅₀) and (WBC₄₀₀₀/ED₅₀) of this compound are very high and its water-solubility is good. In view of these characteristics, this compound is far superior to mitomycin C. The following experiments reveal the pharmacological characteristics of Compounds I of the present invention. EXPERIMENT 1 Certain Compounds I were selected. Table 3 indicates their anti-bacterial activities in terms of their minimum inhibitory concentration (µg/ml), determined by the agar dilution method effected at a pH of 7.0. In this table, the microorganisms used are abbreviated as follows:- SF: Streptococcus faecalis ATCC 10541 SA: Staphylococcus aureus ATCC 6583P EC: Escherichia coli ATCC 26 PV: Proteus vulgaris ATCC 6897 KP: Klebsiella pneumoniae ATCC 10031 No. SF SA EC PV KP 1 1.6 0.8 > 50 0.8 0.4 2 100 50 - 25 > 100 3 3.1 1.6 50 0.8 0.8 4 3.1 1.6 250 3.1 0.8 5 1.6 1.6 - 1.6 0.8 6 3.1 0.8 > 50 0.8 0.8 7 0.8 0.4 2.5 0.8 0.4 MM-C 0.08 0.04 10 0.02 0.02 Note: MM-C mitomycin C. EXPERIMENT 2 Certain compounds were selected from Compounds I. The following Table 4 indicates anti-tumour activity against Sarcoma 180 solid tumour (ED₅₀), acute toxicity (LD₅₀) and effects on the peripheral leucocytes (WBC₄₀₀₀) of each compound. No. LD₅₀ ip (mg/kg) ED₅₀ ip (mg/kg) (C.I.) (WBC₄₀₀₀/ED₅₀) 1 22.5 2.7 2.58 4.12 2 90.0 18 1.55 2.14 3 22.5 5.7 1.46 3.02 4 22.5 5.1 1.52 1.27 5 30.0 8.4 1.66 3.21 6 26.3 5.0 1.81 2.38 7 45.0 9.8 2.13 2.40 9 37.5 7.7 2.09 0.48 10 75.0 13.0 2.19 2.5 11 75.0 16.9 1.59 1.64 12 75.0 18.4 1.80 5.57 13 30.0 12.2 1.17 2.30 No. LD₅₀ ip (mg/kg) ED₅₀ ip (mg/kg) (C.I.) (WBC₄₀₀₀/ED₅₀) MM-C 1 8.4 2.6-3.9 1 1 MM-C 2 <12.0 5.4 1.16 0.95 Notes:- MM-C 1 (mitomycin C,) MM-C 2 (albomitomycin C) (C.I.) = (LD₅₀/ED₅₀)/[(LD₅₀/ED₅₀) of MM-C1]. (WBC₄₀₀₀/ED₅₀) = (WBC₄₀₀₀/ED₅₀)/[(WBC₄₀₀₀/ED₅₀) of MM-C1]. It was previously known that there are two types of mitomycin A and mitomycin C viz. mitomycin-type and albomitomycin-type substances [cf. Abstracts, the 27th Symposium on the Chemistry of Natural Products pp. 672-679, (1985), Hiroshima]. As disclosed in Example 1 hereinafter, it has been found that these two types are also present in various mitomycin derivatives of the present invention. The results shown in Table 4 were obtained by the following experiments:- (1) Effect against Sarcoma 180 solid tumour: 5 X 10⁶ cells of Sarcoma 180 solid tumour cells were abdominally implanted into male mice of ddy strain. 7 days after this, ascites cells were sampled. The cells were washed once with a sterilized physiological solution of sodium chloride and were used to prepare a cell suspension containing 5 X 10⁷ cells per ml. On each occasion, 0.1 ml of the cell supension was subcutaneously implanted into the right armpit of a male mouse (ddy strain: body weight 20 ± 2 g). 24 hours after the implantation of the tumour cells, the test compound which was dissolved in a physiological solution of sodium chloride with or without addition of Tween 80 was abdominally administered to each mouse of a group consisting of 5 animals at a dose of 0.1-0.2 ml, The anti-tumour activity was determined as follows:- 7 days after the implantation, the major axis (a) and the minor axis (b) of the tumour were measured to calculate a value of "a X b²/2" which corresponds to the volume of the tumour. The anti-tumour activity was expressed by T/C viz. the ratio of the volume of the tumours (T) of the group of animals administered with the test compound to the corresponding volume of tumours (C) of the untreated animals. (2) Determination of ED₅₀: The term ED₅₀ denotes the administered amount needed for reducing the volume of Sarcoma 180 solid tumours in the animals to 50 % on the basis of the corresponding volume of Sarcoma 180 solid tumours in control (untreated) animals. On graph paper, T/C was indicated by an arithmetic scale on the longitudinal axis and the administered amount of test compound was indicated by a logarithmic scale on the lateral axis. The relationship between the dose and T/C was shown by a straight line determined by the method of least squares, from which a dose corresponding to T/C of 0.5 was obtained. (3) Acute toxicity: Each animal of the test group consisting of 5 ddy mice was once administered (i.p.) with a test compound. After this, the animals were observed for 14 days to note the survival ratio. The LD₅₀ was determined by Behrens Körber's method. (4) Effect upon the number of the peripheral leucocytes: Sarcoma 180 solid tumour cells (5 X 10⁶) were subcutaneously implanted into the right armpit of each mouse (body weight 20 ± 2 g) of a group consisting of 5 male mice (ddy strain). 24 hours after implantation, a test compound was abdominally administered to each mouse. 4 days later, blood (each 0.02 ml) was collected from the suborbital plexus vein of each tumour-carrying mouse. The collected sample of blood was dispersed in 9.98 ml of Cell-kit Seven solution. One drop of saponin solution was added to the sample to dissolve erythrocytes and then a microcell counter was used to measure the number of leucocytes. On graph paper, the number of leucocytes was indicated on the y-axis by an arithmetic scale and the dose of the test compound was indicated on the x-axis by a logarithmic scale. The relationship between the number of peripheral leucocytes and the dosage of the test compound was plotted to obtain WBC₄₀₀₀ viz. the dosage corresponding to 4000 peripheral leucocytes per mm³ (about half of the number of leucocytes of normal mice). (5) Effect upon Leukemia P 388: Ascites cells were collected from a P388 Leukemia-carrying mouse (DBA/2) 7 days after the implantation. The number of the cells of P388 Leukemia in the sample was counted. A physiological solution of sodium chloride was sterilized and used to prepare a cell suspension containing 5 X 10⁶ tumour cells per ml. On each occasion. 0.2 ml of the suspension containing 1 X 10⁶ cells was implanted into the abdominal cavity of each mouse (CDF₁; body weight 20∼25g). 24 hours after implantation, the test compound was once given to each mouse of a test group consisting of 6 mice by abdominal injection. Survival days of all animals were observed for a period of 33 days. The activity of the test samples was determined by the ratio of the average survival days of the treated animals to the average survival days of untreated animals viz. ILS % (Increased Life Span). Table 5 indicates ILS* which is the ratio of ILS % of each of various test compounds to the corresponding ILS % obtained by using mitomycin C as control under the same conditions. No. ILS Dose (mg/kg) 1 >2.30 25 2 >1.34 50 The present invention provides an anti-tumour composition, comprising as active ingredient an effective amount of Compound I. The compound may be used, for example, for injection by dissolving in a physiological solution of sodium chloride, injection solutions of glucose, lactose or mannitol. If desired, the compound may be formulated into a freeze-dried injection agent or injection powder by mixing with sodium chloride according to the Japanese Pharmacopoeia. The composition may contain pharmaceutically acceptable salts, for example, Ringer's solution, additives well-known in the art [e.g.polyethylene glycol, HCO-60 (surfactant, commercial product of Nikko Chemicals K.K., Japan], ethanol and/or carriers (e.g. ribosome and cyclodextrin ). Although the dose may vary, depending upon the ages and symptoms of patients, it is possible to administer to humans, for example, once to three times per week at a dose of 0.06 to 6 mg/kg usually by intravenous injection. If desired, it is possible to administer the same amount into the artery, abdominal cavity or thorax cavity by injection. It is also possible to administer by oral or rectal route. In such cases, the compound may be added to suitable additives and formulated into tablets, powders, granules, syrups, suppositories and the like. The compounds have, in general, higher water-solublity or lipophilicity and lower toxicity than mitomycin C so that it is possible to provide excellent anti-tumour compositions. BEST MODE EMBODIMENTS FOR CARRYING OUT THE INVENTION : The following examples and references illustrate the present invention, in which the physico-chemical characteristics were determined by means of the following instruments:- IR : JASCO IR-810 (commercial product of Nihon Bunkou K.K., Japan) NMR : Bruker AM400, Varian EM 390 and JEOL FX 100 MS : Hitachi M-80B (commercial product of Hitachi Seisakusho, Japan) Optical rotation : Perkin-Elmer 141 Polarimeter EXAMPLE 1 503 mg of 7-N-[2-(2-pyridyl)dithioethyl]mitomycin C (disclosed in EP 0116208A1 and Japanese laid open Patent Application 175493/84) was dissolved in 15 ml of methanol. An aqueous solution (15 ml) containing 200 mg of γ-L-glutamylcysteamine prepared by the method described in Reference 2 hereinafter was dropwise added to the starting material with stirring at room temperature. Water (120 ml) was then added thereto. The resultant reaction solution was applied to a column packed with Diaion HP-20 (100 ml; commercial product of Mitsubishi Kasei Kogyo K.K., Japan). Elution was effected by the use of a solvent system of water/methanol (300 ml; 7:3 v/v). A further elution was effected using a solvent system of water/methanol (300 ml; 4:6 v/v) to give blue fractions. The blue fraction was concentrated under reduced pressure, followed by freeze-drying to obtain Compound 1 (263 mg) with a yield of 44 %. The resultant product was a mixture of mitomycin-type (A) and albomitomycin-type (B) substances at a molar ratio of 95:5. (mitomycin-type) (albomitomycin-type) Compound 1 exhibited the following physico-chemical characteristics:- SIMS: m/s 599(M+1)⁺, 600 (M+2)⁺, 601 (M+3)⁺ (Molecular formula C₂₄H₃₄N₆O₈S₂ ; M.W. 598.7) By the SIMS method, the ionization is made by collision of Xe⁺ with the sample mixed with glycerol. In such cases, the quinone moiety of mitomycin is reduced by the action of the hydrogen radical originating from glycerol so that the mass number of the compound of the present invention may increase by 2. Moreover, there is a possibility of adding hydrogen ion and hydrogen radical to the target, as sometimes found in the case of usual El mass spectrometry. Thus, the molecular formulae of the compounds of the present invention are highly credible on the ground that (M+2)⁺ and (M+3)⁺ ions were observed (a similar example is disclosed in Japanese laid open Patent Application as 33880/85). Elemental analysis: as C₂₄H₃₄ N₆O₈S₂·H₂O C(%) H(%) N(%) Calculated 46.74 5.88 13.63 Found 46.51 5.97 13.60 ¹H-NMR (400 MHz): (D₂O) δ 1.94* (s), 2.00 (3H, s), 2.15 (2H, m), 2.44 (2H, m), 2.86 (2H, t, J=6.3Hz). 3.00 (2H, t, J=6.3Hz), 3.02 (1H, br.), 3.05 (1H, br.), 3.30 (3H, s), 3.45*(s), 3.53 (2H, t, J=6.3Hz). 3.65 (1H, br.d), 3.65 (1H, dd, J=10.7, 4.5 Hz), 3.78 (2H, t. J=6.2Hz), 4.00 (2H , t, J= 6.3Hz), 4.19 (1H, br. d.J=13.7Hz), 4 .26 (1H, t. J=10.7Hz), 4.60 (1H, dd. J=10.7, 4.5Hz) [Note:- In this specification, ¹H-NMR marked with * denotes a signal originating from albomitomycintype substance] IR : (KBr)cm⁻¹ 3450, 3300, 3080, 2950, 1707, 1631, 1560, 1510, 1460, 1328, 1062 Solubility in water: >20 mg/ml A highly purified mitomycin-type Compound 1 was obtained in the following manner:- Compound 1 (45 mg: powders) was dissolved in 1.8 ml of distilled water. The solution was applied to preparative high performance liquid chromatography using a column packed with a carrier YMC D-ODS-7 (commercial product of Yamamura Kagaku K.K., Japan)]. Elution was effected by using a solvent system of water/acetonitrile (3:1 v/v) at a flow rate of 9.6 ml per minute. Blue fractions which were observed 11.7- 13.5 minutes after the beginning of elution were collected and combined. The combined fractions were freeze-dried to obtain grayish blue powders (33 mg). The resultant powders were subjected to high performance liquid chromatography using a column packed with YMC AM-312 (commercial product of Yamamura Kagaku K.K., Japan) and a solvent system of water/methanol (1:1 v/v). Elution was effected at a flow rate of 1 ml per minute. At a retention time of 6.0 minute, a single peak at 254 nm and a ratio of areal strength of >99 % were noted. The resultant mitomycin-type compound showed the following physico-chemical characteristics:- SIMS: m/z 599 (M+1)⁺, 600 (M+2)⁺, 601 (M+3)⁺ (Molecular formula C₂₄H₃₄N₆O₈S₂ ; M.W. 598.7) ¹H-NMR (400 MHz): (D₂O) δ 2.00 (3H, s), 2.15 (2H, m), 2.44 (2H, m), 2.86 (2H, t, J=6.3 Hz), 3.00 (2H, t, J=6.3Hz), 3.02 (1H, br.), 3.05 (1H, br.), 3.30 (3H, s), 3.53 (2H, t, J=6.3Hz), 3.65 (1H, br. d), 3.65 (1H, dd, J=10.7, 4.5HZ), 3.78 (2H, t, J=6.2 Hz), 4.00 (2H, t, J=6.3Hz), 4.19 (1H, br.d, J= 13.7Hz), 4.26 (1H, t, J=10.7Hz), 4.60 (1H, dd, J=10.7, 4.5Hz), IR : (KBr)cm⁻¹ 3450, 3300, 3080, 2950, 1707, 1631, 1560, 1510, 1460, 1328, 1062 A highly purified albomitomycin-type compound was obtained in the following manner:- 7.8∼8.4 minutes after the beginning of the above-mentioned preparative high performance liquid chromatography, colourless fractions were eluted. The collected fractions were immediately frozen using dry ice/acetone to give slightly blueish powders (1.5 mg). The resultant powders were determined in a similar manner to that described above to give a main peak at a retention time of 4.2 minutes. There was obtained a ratio of areal strength of 95 %. The physico-chemical characteristics of this compound were as follows:- SIMS: m/z 599 (M+1)⁺, ¹H-NMR (400 MHz): (D₂O) δ 1.92 (3H, s), 2.14 (2H, m), 2.43 (2H, m), 2.70 (1H, d), 2.84 (1H, d) 2.85 (2H, m), 2.95 (1H, d), 3.00 (2H, m) 3.02 (1H, d), 3.44 (3H, s,), 3.51 (2H, m.),3.53 (2H, t), 3.76 (1H, t), 3.82 (1H, m), 3.94 (1H, m), 4.31 (1H, dd), 4.41 (1H, dd) IR : (KBr)cm⁻¹ 3400, 3350, 3080. 2950 , 1705, 1630, 1570, 1510, 1450, 1400, 1335, 1240 EXAMPLE 2 A similar treatment to that described in Example 1 was carried out except the use of 7-N-[2-(2-pyridyl)-dithioethyl]mitomycin D (503 mg) disclosed in EP 0116208A1 and Japanese laid open Patent Application 175493/84) instead of 7-N-[2- (2-pyridyl)dithioethyl]mitomycin C to obtain 275 mg of Compound 2 with a yield of 46 %. The physico-chemical characteristics of the resultant compound were as follows:- SIMS: m/z 599 (M+1)⁺, 600 (M+2)⁺, 601 (M+3)⁺ (Molecular formula C₂₄H₃₄N₆O₈S₂ ; M.W. 598.7) As discussed in Example 1, these data make the molecular formula of this compound highly credible. Elemental analysis: as C₂₄H₃₄ N₆O₈S₂·H₂O C(%) H(%) N(%) Calculated 46.74 5.88 13.63 Found 46.59 5.82 13.39 ¹H-NMR (400 MHz): (D₂O) δ 1.98 (3H,s), 2.11 (2H,m), 2.30 (3H, s), 2.42 (2H, m), 2.60 (1H, d, J=4.9Hz), 2.70 (1H, dd. J=4.9, 2.0Hz), 2.84 (2H, t, J=6.2Hz), 3.00 (2H, t, J=6.3Hz), 3.51 (2H, t, J=6.3Hz), 3.65 (1H, dd, J= 13.7, 2.1Hz), 3.73 (1H, dd, J=9.3, 3.6Hz), 3.75 (1H, t, J=6.2Hz), 4.02 (2H, t, J=6.3Hz), 4,08 (1H, d, J=13.6Hz), 4.40 (1H, dd, J=10.9, 9.3Hz), 4.68 (1H, dd, J=10.9, 3.6Hz)) IR : (KBr)cm⁻¹ 3410, 3300, 3100, 2950, 1705, 1629, 1545, 1509, 1467, 1412, 1333 Solubility in water: >5 mg/ml EXAMPLES 3-13 Compounds 3-13 were respectively obtained in a similar manner to that described in Example 1. SIMS,IR and ¹H-NMR of each compound are shown in Table 6 hereinafter. Although all compounds are soluble in water, Compounds 3, 4, 5, 6, 7 and 9 exhibited a water-solubility of >20 mg/ml. Table 6-2 indicates the molar ratio of mitomycin-type substance (A) to albomitomycin-type substance (B), which were isolated from each of Compounds 3 to 9. Compound A B 3 94 6 4 87 13 5 96 4 6 96 4 7 92 8 8 95 5 9 97 3 13 > 99 <1 EXAMPLE 41 Synthesis of Compound 6 (another method): Compound C (600 mg) obtained by the method described in Reference 3 hereinafter was dissolved in dichloromethane (28 ml). To this solution was added anisole (2.8 ml). After addition of trifluoroacetic acid (28 ml) and trifluoromethane sulfonic acid (1.68 ml) at a temperature of 0°C. the mixture was stirred for 30 minutes, followed by further stirring at room temperature for 4 hours. To the reaction solution was added water (50 ml). Then the solution was extracted three times with ether (each 50 ml). The aqueous layer was concentrated under reduced pressure. To the concentrated solution was added a phosphate buffered-solution (M/10; pH 7.5; 30 ml), followed by addition of a small amount of an aqueous solution of saturated sodium bicarbonate to give a pH of 5.5. The reaction solution was applied to a column packed with Diaion SP-207 (100 ml; commercial product of Mitsubishi Kasei Kogyo K.K., Japan ). After washing the column with water (200 ml), elution was effected using a solvent system of water/methanol (300 ml; 5:95 ∼10:90 v/v). The resultant fractions were collected, combined and concentrated under reduced pressure. The concentrate was freeze-dried to give crude powders of N-( β -L-aspartyl)cysteamine (130 mg) with a yield of 60 %. The powders were not purified further but were used for the reaction with 7-N-[2-(2-pyridyl) dithioethyl]mitomyein C (340 mg). The reaction was effected in a similar manner to that described in Example 1 to obtain 168 mg of Compound 6 with a yield of 42 %. EXAMPLE 42 Injection agent: A compound prepared by the method of Example 1 viz. Compound 1 (20 g) and purified mannitol (40 g) were dissolved in a sterilized water for injection and made up to 20 litre in total. The solution was divided into fractions (each 5 ml). Each fraction was put into a brown vial and freeze-dried in conventional manner to obtain a freeze-dried injection agent (5 ml per vial). REFERENCE 1 Synthesis of Compound "a": 859 mg of N,N'-bis[N-carbobenzyloxy- γ - (α - benzyl-L-glutamyl]cystamine (disclosed in Japanese laid open Patent Application 4121/76) was dissolved in dichloromethane (25 ml). To this solution was added anisole (2.5 ml)while cooling with ice, followed by addition of trifluoromethanesulfonic acid (1.5ml). The mixture was stirred for 30 minutes while cooling with ice, followed by further stirring at room temperature for one hour. The reaction solution was concentrated under reduced pressure. Then decantation was effected twice by using ether (each 30 ml). The residue was dissolved in a buffer solution (50 ml) of M/15 Na₂HPO₄-M/15 K₂HPO₄ (1:1 v/v) and applied to a column packed with Diaion SP-207 (50 ml: commercial product of Mitsubishi Kasei Kogyo K.K., Japan). After washing the column with water (150 ml), a solvent system of water/methanl (100 ml; 4:1 v/v) was passed through the column. The resultant fractions were collected, combined, concentrated under reduced pressure, and freeze-dried to obtain N.N'-bis-( γ -L-glutamyl)cystamine viz. Compound "a" (402 mg) with a yield of 98 %. The physico-chemical characteristics of the resultant compound as trifluoroacetate were as follows: SIMS : m/z 411(M+1)⁺, (Molecular formula C₁₄H₂₆N₄O₆S₂ : M.W. 410.5) ¹H-NMR (400MHz) : (D₂O) δ 2.18(4H, m), 2.45(4H, m), 2.84 (4H, m), 3.51 (4H,m,), 3.67 (2H, m), IR : (KBr) cm⁻¹ 3450, 3100, 2940, 1680, 1638, 1200, 1136, Optical rotation: [α ] 27 / D +11.5° (c 0.47, H₂O) REFERENCE 2 Synthesis of Compound "b": Compound "a" (205 mg) described in Reference 1 was dissolved in water/ethanol (20 ml; 3:1 v/v). After adding ethanethiol (3 ml), the mixture was stirred at room temperature for 3 days. The solvent was removed by evaporation under reduced pressure. The reside was applied to a column packed with Diaion SP-207 (25 ml). Water (75 ml)was passed through the column. Then elution was effected by using a solvent system of water/methanol (50 ml; 9:1 v/v). The eluted fractions were collected, combined and concentrated under reduced pressure, followed by freeze-drying to obtain γ -L-glutamylcysteamine (Compound "b") (190 mg) with a yield of 92 %. This compound showed the following physico-chemical characteristics:- SIMS : m/z 207(M+1)⁺, (Molecular formula C₇H₁₄N₂O₃S; M.W. 206.3) ¹H-NMR (400MHz) : (D₂O) δ 2.18(4H, m), 2.46(4H, m), 2.62 (4H, m), 3.34 (4H,m,), 3.72 (2H, m), IR : (KBr) cm⁻¹ 3400, 3100, 2940, 1640, 1530, 1410, 1205, 1135, Optical rotation: [α ] 27 / D +11.2° (C 0.99, H₂O) REFERENCE 3 Synthesisof Compound "c": N-carbobenzoxy-α -benzyl-L-aspartic acid (1.617 g; commercially available from Kokusan Kagaku K.K., Japan, 2509181) was dissolved in dichloromethane (20 ml). To this solution were added N-hydroxy-succinimide (522 mg) and dicyclohexylcarbodiimide (933mg). The mixture was stirred at a temperature of 0 °C for 2 hours and further stirred at room temperature for 18 hours. Then, to the reaction solution were added 2-[(p-methoxybenzyl)thio]-ethylamine hydrochloride (1 g) and triethylamine (623 µ l). The mixture was stirred at room temperature for 2 hours. The resultant precipitates were separated by filtration. After addition of an aqueous solution of 10 % citric acid (30 ml), the reaction mixture was extracted with ethyl acetate (150 ml). The extracted solution was washed with 5 % sodium bicarbonate solution (30 ml) and a saturated solution of sodium chloride (30 ml) and was then dried by using sodium sulfate. After removing the solvent by evaporation under reduced pressure, the residue was subjected to silica gel chromatography using a column packed with silica gel (150 g; Wako Gel C200). Elution was effected by a solvent system of chloroform/methanol (99:1 v/v) to obtain N-[N-carbobenzoxy-β-(α-benzyl)-L- aspartyl]-S-(p-methoxybenzyl)cysteamine viz. Compound "c" (1.929 g) with a yield of 80 %. The physico-chemical characteristics of the product were as follows:- SIMS : m/z 537(M+1)⁺, (Molecular formula C₂₉H₃₂N₂O₆S; MW 536.6) ¹H-NMR (400MHz) : (CDCl₃) δ 2.50(2H, t, J=7Hz), 2.78 (2H, AB, J=16.4Hz). 3.33 (2H, q, J=7.0Hz), 3.64 (2H, s), 3.79 (3H, s,), 4.63 (1H, dt, J=8.5, 5.4Hz), 5.12 (2H, s), 5.19 (2H, s), 5.83 (1H, br), 6.07 (1H, br. d), 6.88 (2H, d. J=9.0Hz), 7.24 (2H, d, J=9.0Hz), 7.36 (10H, s) IR : (KBr) cm⁻¹ 3300, 2900, 1735, 1695, 1635, 1535, 1510, 1285, 1250, 1065, 745, 695 REFERENCE 4 The following compounds were obtained in a similar manner to that described in Reference 3 except the use of the following compounds instead of 2-[(p-methoxybenzyl)-thio]ethylamine:- homocystamine (Merck Index, 10th Edit., 2771, 1983) for the preparation of Compound "d"; bishomocystamine (disclosed in Japanese Patent Publication 54314/81) for the preparation of Compound "e" ; and cystamine for the preparation of Compounds "f" and "g". In the reactions, it was necessary, in general, to double the molar amount of the protected amino acid disclosed in Reference 3. Pyroglutamic acid was used for the preparation of Compound "f". The SIMS, IR and ¹H-NMR data of the resultant compounds are indicated in Table 13 hereinafter. Compound "d": N,N'-bis[N-carbobenzoxy-γ -(α -benzyl)-L-glutamyl]homocystamine Compound "e": N,N'-bis[N-carbobenzoxy-γ -(α -benzyl)- L-glutamyl]bishomocystamine Compound "f": N,N'-bis(L-pyroglutamyl)cystamine Compound "g": N,N'-bis(N-t-butyloxycarbonyl-L-prolyl)-cystamine By the similar methods to those described in References 1 and 2, the desired mercaptan III was obtained by using Compounds "d "and "e " respectively. In a similar manner to that described in Reference 2, the desired mercapatan III was obtained from Compound "f". Compound "g" was treated with trifluoroacetic acid and then treated in a similar manner to that described in Reference 2 to obtain the desired mercaptan III. REFERENCE 5 The following compounds were obtained in a similar manner to that described in Reference 3. Their SIMS, IR and ¹H-NMR data are shown in Table 14 hereinafter:- Compound "h": N-[N-t-butyloxycarbonyl-α -(β -benzyl)-L-aspartyl]-S-(p-methoxybenzyl)cysteamine Compound "i": N-[N-t-butyloxycarbonyl-α -(γ -benzyl)-L-glutamyl]-S-(p-methoxybenzyl)cysteamine Compound "j": N-[N-carbobenzoxy-α -(γ -benzyl)-L-glutamyl]-S-(p-methoxybenzyl)cysteamine Compound "k": N-[N-carbobenzoxy-γ -(α -benzyl)-D-glutamyl]-S-(p-methoxybenzyl)cysteamine The desired mercaptan was obtained in a similar manner to that described in Reference 1 by using these compounds. INDUSTRIAL APPLICABILITY OF THE INVENTION: Mitomycin derivatives according to the present invention may be used as anti-tumour agents with respect to their anti-tumour activities which are, in general, higher than the anti-tumour activity of mitomycin C as well as to their lower toxicity and they have high solubility in water or lipid. No SIMS (m/z) IR(KBr)cm⁻¹ ¹H-NMR(CDCℓ₃)δ (400MHz) h 503 (M+1)⁺ 3350, 2970, 1735, 1680, 1660, 1515, 1370, 1300, 1250, 1170, 1030, 1005, 735 1.46(9H,s), 2.48(2H,t,J=7Hz), 2.70(1H, dd,J=16.6Hz), 3.04(1H,dd,J=16.5Hz), 3.35 (2H,q,J=7Hz), 3.63(2H,s), 3.76(3H,s), 4.45(1H,m), 5.09(2H,s), 5.61(1H,br.d), 6.75(1H,br.), 6.79(2H,d,J=8Hz), 7.19(2H, d,J=8Hz), 7.31(5H,s) i 517 (M+1)⁺ 3360, 3330, 2930, 1720, 1680, 1650, 1505, 1285, 1245, 1195 805, 745, 670 1.41(9H,s), 2.00(2H,m), 2.50(4H,m), 3.35 (2H,q,J=7Hz), 3.62(2H,s), 3.74(3H,s), 4.09(1H,m), 5.10(2H,s), 5.20(1H,m), 6.42 (1H,br.s), 6.79(2H,d,J=8Hz), 7.17(2H,d, J=8Hz), 7.30(5H,s) j 551 (M+1)⁺ 3295, 2950, 1730, 1690, 1645, 1535, 1510, 1255, 1170, 1050, 1030, 815, 745, 700 2.07(2H,m), 2.50(4H,t,J=7Hz), 3.34(2H,q, J=7Hz), 3.62(2H,s), 3.75(3H,s), 4.24(1H, q,J=7Hz), 5.09(4H,s), 5.78(1H,d,J=7Hz), 6.75(1H,br.), 6.83(2H,d,J=9Hz), 7.22(2H, d,J=9Hz), 7.33(10H,s) k 551 (M+1)⁺ 3310, 2930, 1730, 1695, 1635, 1540, 1515, 1275, 1240, 1205, 1185, 1170, 1065, 1030, 830, 755, 735, 700 2.17(4H,br.), 2.52(2H,t,J=6Hz), 3.34(2H, q,J=6Hz), 3.44(3H,s), 3.64(2H,s), 4.43 (1H,m), 5.10(2H,s), 5.16(2H,s), 5.67(1H, br.s), 5.93(1H,br.s), 6.82(2H,d,J=9Hz), 7.20(2H,d,J=9Hz), 7.33(10H,s)

Claims: Mitomycin derivatives represented by the formula: wherein X' and X'' each independently represents hydrogen or C₁₋₄ alkyl; Y and Z each independently represents hydrogen or methyl; provided that when Z is hydrogen, Y is methyl; one of R₁ and R₂ represents carbamoyloxymethyl and the other represents hydrogen; n represents an integer of from 1 to 4; and X is R₃NH, R₃O or R₃S wherein R₃ is the residue of an α-amino acid from which OH of the carboxyl group has been removed, provided that when said α-amino acid contains a second carboxyl group, the second carboxyl group may be protected by C₁₋₄ alkyl or may form a salt with an alkali metal, ammonium or organic amine. Albomitomycin derivatives represented by the formula: wherein X, X', X'', R₁, R₂, Y and n are as defined in claim 1. Mitomycin derivatives as claimed in claim 1 or claim 2 wherein the amino group of said α-amino acid is protected by C₁₋₄ alkanoyl or forms a salt with an inorganic or organic acid. Mitomycin derivatives as claimed in any one of claims 1 to 3 wherein R₃ is glutamyl or aspartyl. Mitomycin derivatives as claimed in claim 4 wherein n is an integer of from 2 to 4. A compound as claimed in claim 1 being 7-N-[2-[[2-(N⁵-L-glutamino)ethyl]dithio]ethyl]-mitomycin C. A compound as claimed in claim 2 being Pharmaceutical compositions comprising a pharmacologically effective amount of a compound as claimed in any one of the preceding claims in association with at least one pharmaceutically acceptable carrier and/or adjuvant. A compound as claimed in any of claims 1 to 7 for use as an anti-tumour agent in a mammal. Use of a compound as claimed in any of claims 1 to 7 for the manufacture of a medicament for anti-tumour therapy in a mammal.

Inventors: ASHIZAWA TADASHI, KANDA YUTAKA, KASAI MASAJI - - MATSUGAOKA, KONO MOTOMICHI, MORIMOTO MAKOTO, SAITO YUTAKA, SATO AKIRA, ASHIZAWA, TADASHI, KANDA, YUTAKA, KASAI, MASAJI 3-12-15, MATSUGAOKA, KONO, MOTOMICHI, MORIMOTO, MAKOTO, SAITO, YUTAKA, SATO, AKIRA
Applicants: KYOWA HAKKO KOGYO KK, KYOWA HAKKO KOGYO KABUSHIKI KAISHA
Application year: 1987
Main classification: C07D487
IPC codes: C07D487, C07H19, C07J43