2-(AMINOALKYLTHIO)METHYL-1,4-DIHYDROPYRIDINE, A METHOD FOR THE PREPARATION THEREOF AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM

Abstract: Compounds of formula (I), wherein P and P1 groups represent hydrogen, alkyl, heteroalkyl, aryl or aralkyl groups, optionally substituted or forming a ring, n is an integer between 0 and 2, m is an integer from 1 to 3, R1 and R3, that may be the same or different, represent alcoxycarbonyl groups, CN, NO2 or acyl groups, whereas R2 is an aryl group or a heteroaryl group that may be optionally substituted, are useful as active principles of pharmaceutical compositions.

Description: "2-(AMINOALKYLTHIO)METHYL-l.4-DIHYDROPYRIDINE. A METHOD FOR THE PREPARATION THEREOF AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM" Object of the present invention are 2—(formylaminoalkylthio)-methyl-1,4-dihydropyridine, a method for the preparation thereof and pharmaceutical compositions containing them. The compounds object of the present invention have the following general formula I wherein: - R.. represents: - . a COCH COC H CN or NO group; - . a COORa group, wherein Ra represent hydrogen, C.-C- i 6 straight or branched alkyl, optionall bearing one or more C -C alkoxy groups and/or secondary amino groups of formula -NR 4 wherein R and R that may be the same or different, represent C J...-Cb- alkyl, phenyl, benzyl or taken, together with the nitrogen atom, form a five or six merbered ring optionally containing other atoms; - R is a phenyl ring unsubstituted or substituted with one or more C^-C alkyl, halogen, nitro, cyano, C -C alkoxycarbonyl, C 1 -C 8 alkylthio, C -C alkylsulfynyl; pentafluorophenyl; c - or β-naphtyl; a five- or six-membered heterocyclic ring; ^-benzo[2.3-b]-1.4- -diσxran-OC-yl; C\-benzofuroxanyl; - R represents a COORa group wherein Ra is as above defined; - P is selected from the group consisting of hydrogen, -(CR,,) -W and C.-C 0 linear or branched alkyl; 2 p - o - N ^ is the residue of a primary or secondary amino group wherein P.. is selected from the group consisting of hydrogen, C.-C lower linear or branched alkyl group -L 6 and -(CH 2 )p-W; - P, taken together with P. and the nitrogen atom to which P is linked, may form a pyrrolidine or a piperidine ring; - W is selected from the group consisting of hydroxymethyl, formyloxymethyl, CO R wherein R is hydrogen or C.-C. lower alkyl, CN^ saturated or unsaturated heterocyclic ring, C -C cycloalkyl ring, phenyl ring optionally substituted by one or more halogens or C. -C -alkoxy groups; - m is an integer from 1 to 3; - n is zero or an integer from 1 to 2; - p is zero or an integer from 1 to 3. Also the pharmaceutically acceptable salts as well as the optical antipodes, i.e. the enantiomers, the possible geometric isomers, diastereoisomers and mixtures thereof are included in the scope of the present invention. The alkyl, alkenyl, alkoxy and alkanoyloxy groups are branched or straight chain groups A halo-C.-C alkyl group is preferably trihalo -L D -C.-C- alkyl, in particular trifluoromethyl. 1 6 A halo -C -C alkoxy group is preferably -OCHF_. A C -C C alkyl group is preferably methyl, ethyl, 1 o isopropyl or t-butyl. An aryl group is preferably phenyl. A C,-C_ alkenyl group is preferably allyl. A C_-C_ alkynyl group is preferably propargyl. A C -C cycloaliphatic group is preferably cyclopentyl, cyclohexyl or cycloheptyl. A monoalkyl amino group is preferably a methyl-, ethyl-, isopropyl- or benzyl-amino group. A C..-C- alkoxy is preferably methoxy or isopropoxy. A C,-C_ alkylthio is preferably methylthio or isopropylthio. A c - ι -C- alkoxycarbonyl is preferably methoxy-, ethoxy- or ter-butoxy-carbonyl group. When R is a five- or six- membered heterocyclic ring, it is preferably pyridyl, furanyl or thienyl. The non toxic salts, that are pharmaceutically acceptable, include the hydrochlorides, hydrobromides , hydroiodides, (lower)alkylsulfates, (lower)alkyl and aryl sulfonates, phosphates, sulfates, maleates, fumarates, succinates, tartrates, citrates, and others commonly used in the art. The salts obtained through the variation of the acid used in some cases have special advantage due to increased stability, increased solubility, decreased solubility, ease of crystallization, lack of objectionable taste, etc., but these are all subsidiary to the main physiological action of the free base, which is independent on the character of the acid used in the preparation of the salt. Specific examples of preferred compounds of the invention are: a 4-(3-nitrophenyl) , (3-chlorophenyl) ,- (3-cyanophenyl) , (3-methoxyphenyl) , (4-fluorophenyl) , (3-methylthio- phenyl) , (Q( -benzo[2,3-b] - 1, 4-dioxan-c( -yl) , (2-fluoro-5-methylthiophenyl)- 2-(2-formylaminoethyl- thio)methyl-3-ethoxycarbonyl-5-methoxycarbonyl-6-me- thyl- 1,4-dihydropyridine. a diastereoisomer of 2-(2-formylamino-2-phenylethylthio) methyl-3-ethoxycarbonyl-5-methoxy carbonyl-4-(m-nitro- phenyl)-6-methyl-l,4-dihydropyridine. a diastereoisomer of 2-(N-formyl-pyrrolidin-2-yl methyl- thio)methyl-3-ethoxycarbonyl-5-methoxycarbonyl-4- -(m-nitrophenyl)-6-methyl-l.4-dihydropyridine. - 2-(2-formylaminoethylthio) methyl-3-ethoxycarbonyl-5- -(2-N,N-dimethylamino)ethoxycarbonyl-4-(m-chlorophe- nyl)-6-methyl-l,4-dihydropyridine. - 2-(2-formylaminoethylthio)methyl-3-methoxyethoxycarbo- nyl-5-methoxycarbonyl-4-(3-methylthiophenyl)-6-methyl- -1,4-dihydropyridine. - 2-[2-N—(2-cyanoethyl)-N~formylaminoethylthio]methyl- -3-ethoxycarbonyl-5-methoxycarbonyl-4-(m-nitrophenyl)- -6-methyl-l,4-dihydropyridine. - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5- -(2-N-methyl-N-benzylamino)ethoxycarbonyl-4-(m-nitro¬ phenyl)-6-methyl-l, 4-dihydropyridine. The compounds of the invention are obtained by a process comprising a) forming a thioether bond by reaction of a compound of general formula with a compound of formula III Y -(CH_) -CH-N 1 2 m \ wherein: CHO (III) R R R m, P, N-P are as above defined; - either Y or Y* represents a thiol or a masked thiol group such as thio C--C. _ alkanoyl ester or a thiouronium salt -S-(C=NR_)NR_R 0 y„ the other being a known leaving group such as chlorine, bromine, iodine, trifluoromethane sulphonate, an alkyl- or an arylsulphonate; - R c ι R_, R 0 , which are the same or different, are hydrogen or a C.-C. alkyl group and y_ (-) i.s a pharmaceutically acceptable anion; b) σyclizing a compound of formula IV with an alkylidene compoun o formula V < > wherein R. , R R P, NP m, are as above defined. c) cyclizing a compound of formula VI R -,—1 N CH — (CH ±-) — S —H Δ C' NH Δ„ (VI) CHO with an alkylidene compound of formula VII wherein . is preferably CO Ra, CN, COCH COC H . d) formylating a compound of formula VIII wherein R.. , R_ , R P, P. and n are as above defined. The above reactions a) - d) give compounds of formula I which may be optionally subjected to further processes such as oxydation, salification, separation of isomers etc. The thioeterification reaction between a compound of formula II and a compound of formula III may be carried out, as known in the art, using almost equimolar amounts of reagents in an inert solvent at a temperature ranging form -20° to +60°C, in the presence of a base. Suitable solvents may be chosen in the group of alcohols, amides, linear or cyclic ethers, ketones esters, halogenated hydrocarbon, whereas the base may be an alkali or earth-alkali metal hydroxide, carbonates, bicarbonates, alcoholates, hydrides , amides or organic bases such us triethylamine, pyridine etc. The selective oxidation of the thioethereal bond of the compounds of formula I where n=0 to give the compounds of formula I wherein n is 1 or 2 is carried out in an inert solvent such as ethyl acetate, ethyl formiate, dichloromethane, 1,2-dichloroethane, chloroform or mixtures thereof, by reaction with one or more molar equivalents of a peracid such as perbenzoic, m-chloro,-perbenzoic, periodic, monoperftalic, peracetic, performic or peroxytrifluoroacetic acid, working in a temperature ranging from -30°C to room temperature. Preferably, the reaction is carried out at above 0°-C. Compounds of formula I wherein n=l are obtained using 1 mol. equiv. of peracids, while using 2 or mor mol. equiv. of peracids, compounds with n=2 are obtained. The cyclization- of an enaminoderivative of formula IV and VI with an ethylenederivative of formula V and VII respectively is the well-known Hantzsch reaction, described by F. Brody and P.R. Ruby in "Pyridines and its derivatives", part I, pages 355-533, A. Weissbenger - Interscience, New York 1960. The formylation reaction of a compound of general formula VIII is also carried out using well-known technique, for instance by treatment in an inert solvent such as dimethyl formamide or dimethyl acetamide, with a 5-10 molar excess of formic acid and heating the mixture for 1 8 h at a temperature ranging from 70 to 120-C. Alternatively, an amino compound of formula VIII is reacted in an inert dry solvent such as tetrahydrofuran, benzene, ethylacetate, dimethylformamide or their mixtures with at least a 0.1 molar excess of N-formyl-imidazole; preferred reaction conditions are from the room temperature to 5°C for a time from 10 minutes to 1-2 hours. Alternatively, a salt of a compound of formula VIII (hydrobromide, nitrate, sulphate or emisulphate) is reacted with an excess of formamide at a temperature ranging from 60 to 90°C for a time from few minutes to 3 hours. The optical resolution processes are preferably carried out on compounds of formula I wherein n is 0 or 2, whereas chiral compounds wherein n is 1 are preferably obtained by oxydation of the antipodes wherein n is zero. The optional resolution process is carried out on diastereoisomeric derivatives of compounds I. For examples, diastereoisomerio salts of compounds I with optically pure acids or bases may be preparared by known salification methods and optionally subjected to optical resolution. When Ra contains an amino group, optically pure acids are used while when Ra is hydrogen, optically active bases are used, to give, after the resolution process, optically pure acids of formula I, which may be optionally esterified by known methods. On the other hand, racemic acids of formula I may be esterified with optically pure alcohols and the obtained diastereoisomeric alcohols may optionally be subjected to resolution by crystallization or chromatographic methods. The obtained optically pure esters may be transformed by known methods into acids or esters I. The compounds of formula II wherein Y is a halogen, have been described in the Application n. 21875 A/85 of August 6, 1985 and the compounds of general formula IV wherein y is a thiol and/or a masked thiol function are described in PCT/EP86/00445. The compounds of formula III, if not already known may be easily prepared from commercialy available compounds, using well-known, safe and unexpensive methods . A general source of compounds III are aminoalcohols IX wherein m, P, Pi are as above defined; said alcohols are commercially available or are prepared by reduction of the corresponding amino-carboxy esters or lactames . Compounds of general formula IX are easily converted into compounds III by known reactions such as formylation and transformation of the alcoholic group into a thiol. Compounds of general formula V are prepared from compounds of general formula X P---P 0 (X) I 1 ! II OHC-N —CH-(CH ) -S-(CH 0 ) -C-CH o -C0_Ra 2 m 2 m 2 2 (P, P X, m and Ra as above defined) by Knoevenagel condensation with aldehyde of formula XI R CHO (XI) (R as above defined) while compounds of formula VII are prepared from compounds of formula X by reaction with ammonia or ammonium salt. Compounds of formula X are prepared, for example, via thioetherification reaction of compounds of formula 87 - 10 - III with- 4-chloro-3-oxo-butanoic acid Compounds of general formula IV and XI are known or easily available. Some formylamino alkyl thiomethyl derivatives of the invention, such as 2-formylamino ethyl thio-4-(m-nitrophenyl)-3-carboethoxy-5-carbomethoxy-6-met hyl-I,4—dihydropyridine show peculiar properties such as low oral acute toxicity and high tolerability in some susceptible experimental animals, e.g. dogs, combined with pronounced and long lasting antihypertensive activity at very low doses (for ex. 0.2 mg/kg/os) when tested orally in conscious SH rats once daily. The antihypertensive effect is dose related in the investigated dose range, for example from 0.05 to 0.8 mg/kg. The maximum hypotensive effect, proportional to the administered dose, takes place 5-7 hours after the administration and the blood pressure is maintained at the decreased level for further 4-5 hours, at least. The gradual onset of the antihypertensive effect does not seem to be coupled with reflex tachicardia, which is often observed after treatment with other antihypertensive agents such as, for example, hydralazine and many dihydropyridines, in the same experimental models. On the contrary, no substantial modifications of the mean B.P. and heart rate are observed after one day oral administration of the same compounds to normotensive conscious rats at the same dose range. After a two weeks treatment consisting of one daily oral administration using a dose range from 0.1 to 0.8 mg/kg, a gradual decrease of mean B.P, also dose-related is observed in the treated conscious SH-rats. Dosages such as 0.2-0.4 mg/Kg are sufficient to stabilize gradually the mean B.P. to lower level for a 24 hrs period. The drop-out of the pharmacological treatment is not combined with an acute hypertensive rebound effect but in the following 2-3 days the mean blood pressure gradually rises to the initial values. A similar pharmacological profile is shared by many 2-amino alkyl thio-methyl-1,4-dihydropyridines of formula III. In general they turn out to be effective antihypertensive agents when orally tested in consciuous SH rats and well-tolerated in subchronic toxicological studies carried out in male and female normotensive rats, independently from the fact that many of these substances show low LD in mice by the oral or intraperitoneal route. Representative results of acute toxicity studies are reported in table 1; the same substances tested orally in conscious SH rats (200-250 g/body weight) show at 1.6 mg/kg a maximum decrease of the mean blood pressure of about 30-80 mmg, with the exclusion of compounds 1, 4, 21, 20, 31 (- BP ranging from 10 to 25 mmHg); 2, 5, 6, 7, 10, 11, 12 (- BP higher than 85 mmHg) , whereas the compound 16 d is practically inactive at 3.2 mg/kg. However, male and female beagle dogs orally treated with 1.5 mg/kg/day (bolus administration) of the compounds 11 and 15 died after three days treatment. CO Me CO Et H 1 CH 2 NH 2 33 31 CO Me CO Et 2-CF 3 1 CH 2 H 2 2 2 CO Et CO Et 2-CF 3 1 CH 2 NH 2 9 5 CO Me CO Et 2-N0 2 1 CH 2 NH 2 3 2 CO Et CO Et 2-C1 1 CH 2 H 2 12 9 CO Me CO Et 2-C1 1 CH 2 NH 2 5 4 CO Me C0 2 Et 3-C1 1 CH 2 NH 2 40 23 CO Me C0 2 Et 2-SCH. 1 CH 2 NH 2 8 5 CO Me C0 2 Et 3-CF 3 1 CH 2 NH 2 40 23 C0 2 Et C0 2 Et 3-N0 2 1 CH 2 NH 2 21 20 CO Et CO Me 3-N0 2 1 CH,NH 2 52 22 C0 2 Et CO Me 3-N0 2 1 CH 2 N ( CH 3 ) 2 38 25 C0 2 Et CO Me 3-N0 2 1 CH 2 NH 2 21 8 C0 2 E CO Me 3-N0 2 1 CH_NH-C .H Q 23 12 2 4 9 C0 2 Et C0 2 Et 3-N0 2 1 CH_NH-C . H Q 40 39 2 4 9 d 1000 1000 C0 2 Et CO Me 3-NO 2 CH NH 22 16 CO Et CO Me 3 -NO 3 CH NH 11 4 p p LD50 in mice mg/ ! - 1 R ι R X m CH-NH OS i.p. 3 9 C0 2 Et CO Et 3-N0 2 1 CH 2 NH-(CH 2 ) 2 CN 95 54 0 C0 2 Et CO Me 3-N0 2 1 CH 2 H-(CH 2 ) CN 63 35 CO Et N0 2 3-N0 2 1 CH 2 NH 2 451 108 CO Et CN 3-N0 2 1 CH 2 NH 2 611 82 CO Et 3-N0 2 1 H 2 NH 2 91 32 C0 2 CH(CH 3 ) 2 C0 2 Et 3-N0 2 1 CH 2 H 2 53 25 C0 2 Me CO Me 3-N0 2 1 CH 2 NH 2 53 25 C0 2 Me CO Et 3-OCH 1 CH 2 NH 2 82 59 C0 2 Me C0 2 Et 4-F 1 CH 2 H 2 46 28 CO Me CO Et 3-CN 1 CH 2 NH 2 34 43 CO Me C0 2 Et 3-N0 2 1 CH(NH_) 77 46 1 2 C 6 H 5 C0 2 Me CO Et 3-N0 2 1 CH(NH_) 42 50 1 2 CH 3 C0 2 Me CO Et 3-N0 2 1 CH-NH 371 87 1 2 CH OH CO Et CO Et 3-N0 2 1 763 354 Acylation of the amino group of a compound of formula VIII, (for ex. the 2-acetyl amino or 2-benzoylamino derivatives of compounds 11, 15) reduces acute toxicities in mice but in general the long lasting antihypertensive action in SH rats is lost and the compounds require almost two administrations dayly in order to stabilize the mean blood pressure values to lower levels for a 24 hours period. Surprisingly, the 2-formyl amino-alkyl-thio methyl-1,4-di- hydropyridines of the present invention do not only show long lasting antihypertensive effect and lower acute toxicities (for ex. 2-formyl-amino-ethyl-thio methyl-4-(m-nitrophenyl)-3-carboethoxy-5-carboethoxy-4-m-ni- trαphenyl-6-methyl-l r 4-dihydropyridine shows >D_ in mice of ZOO and 150 mg/kg orally and i.p. respectively) but are well-tolerated in dogs. Male and female beagle dogs orally treated with the latter substance of the invention at dosages as high as 5-12.5 mg/kg/day (bolus administration) do not die after two weeks treatment. After N-formylation of the side chain amino function of the compound of formula VIII of table I, a 5 to 12 fold acute toxicity reduction in mice is observed. For example, N-formylation of compound 8, increases I>D in mice (oral) from 8 mg/kg to 90 mg/kg. Moreover, the ability of the compounds of the invention to inhibit "in vitro" the contractile activity induced by increasing concentration of calcium ions in K -depolarized rat aorta strips was investigated according to the technique of T. Godfraind et al. (Arch. Int. Pharmacodyn. 172, 235, 1968). Representative results of these studies with compound 11 and its N-acetyl and N—formyl derivative are reported in Table 2. TABLE II 3-ethoxycarbonyl-5-metho- Inhibition of contractile xycarbonyl-4-m-nitro- responses in K depolarized phenyl-1/ 4-dihydropyridines rat aorta strips by increasing Ca concentration. IC (drug concentration ) after different incubation times 2 - 5 min 2-3hrs rao 2-amino-ethyl- -thio 4.15.10 7 9.10.- 10 methyl (as fumarate) (+) antipode S. IC "5 ' l.i.io "7 (-) antipode 1.7.10 "7 2.3.10 -9 rac 2-acetylamino ethyl- •thio- • 1.1.10 "8 8.5.10~ 9 methyl (+) antipode 2.10 "6 1.67.10 "7 -9 (-) antipode 4.1.10 7.49.10 "10 rac 2.-formylaminoethylthio 1.62.10 "8 8.06.10 "10 methyl (+) antipode ιo _5 .ιo- 6 5.3.10 "7 (-) antipode 1.07.10 "8 7.10- 10 nifedipine 2.7.10 "8 2.7.10 "8 The reported results further confirm that better inhibiting activities (ID C- ranging from 10~ to 10~ ) may be observed after longer incubation times with th'e investigated tissue preparations than after shorter ones (ID -5 -9 50 ranging from 10 to 10 ) whereas the behaviour of the standard compound nifedipine is not related to the incubation time. The shorter lasting antihypertensive effect of the N-acetyl compound and the long lasting effects of the compounds 11 and of its N-formyl derivative does not seem to be correlated with "in vitro" experimental results related to the supposed Ca-antagonist potency and capability of relaxing smooth muscles, phenomenon which cannot be easily explained on the basis of the present knowledge. Independently from this aspect, the particular antihypertensive effect, its gradual onset, the long lasting action combined with lower acute toxicity and with increased tolerability in dogs provide evidence that the compounds of the invention are useful in human and veterinary therapy for the treatment of hypertensive situations of different origin, and for the treatment and prevention of cardiovascular and coronary diseases. In order to attain the desired effects in human and veterinary therapy, the compounds of the invention may be administered parenterally, for example by intravenous, hypodermic or intramuscular injection, by infusion, rectally or orally. The compounds may also be administered in pure form or in the form of a pharmaceutical composition. The formulation of suitable pharmaceutical compositions may be prepared according to techniques well-known in the art, such as the ones described in "Remington's Pharmaceutical Sciences Handbook", Hack Publishing Co., U.S.A. When the compounds of the invention are used as antihypertensive drugs,the dosage will vary according to the seriousness of the .hypertension and to the administration route. The amount of the active principle administered may vary, anyhow, between lmcg/kg/die and 1 mg/kg/die, preferably between 5 meg/die and 0.1 mg/kg/die, by the oral route and from 0.1 mcg/kg/die to 0.5 mg/kg/die, preferably from 0.5 mcg/kg/die to 0.2 mg/kg/die, by the parenteral route. A dosage for oral administration may contain, for example, between 50 meg to 10 mg of active principle. The compounds of the invention may be administered even once a day, however more spaced and/or repeated administrations may be, at least in some cases, more suitable and may vary according to the conditions of the patient and to the administration route or to the dosage. By oral administration the compound may be formulated in solid or liquid preparations, such as capsules, pills, tablets, powders, solutions, suspensions or emulsions. By parenteral administration the compound may be administered in injectable formulations, dissolved or suspended in physiologically acceptable diluents, with a vehicle that may be a sterile liquid such as water or an oil, with or without the addition of other excipients. The compounds may also be administered per rectal route, in the form of suppositories, mixed with the conventional vehicles. The preferred administration route of the compounds of the invention is the oral route. The invention is illustrated by the following non limitative examples, wherein the abbreviations EtOH, DME, THF, Et 0, AcOEt, AcOH refer to ethanol, dimethoxyethane, methanol, tetrahydrof ran, ethyl ether, ethyl acetate, acetic acid respectively. Preparation 1 A stirred solution of cysteamine hydrochloride (g 20) in formamide (ml 20) in heated at 75-80°C for 2 hours. After cooling at room temperature, the NH.C1 precipitate is filtered off and washed with a little formamide. The solution of N-formyl cysteamine (about 18 g) in formamide is then diluted with EtOH (160 ml), cooled at 0°C, and in nitrogen atmosphere is treated under stirring with 20% aqueous NaOH (170 ml) and with a solution of ethyl 4-chloro-3-oxo-butanoate (28,4 g) in EtOH (20 ml). After 30 minutes the mixture is poured into water (2000 ml) and extracted with AcOEt (3x200 ml). The combined extracts are 'washed with a saturated solution of NaH PO (3x50 ml) H O (3x100 ml), dried on Na SO and evaporated to dryness in vacuum to give 40.5 g of ethyl 4-(2-formylaminoethylthio) 3-oxo-butanoate as an oil; 1H-NMR (CDCI3) , ^(TMS) :1.10-1.30 (3H, t) ; 2.20-2.60 (4 H, m) ; 3.40-4.10 (6H, m) ; 6.60 (1H, m) ; 8.10 (1H, S) . Using, in the same procedure, the methyl and the methoxyethyl 4-chloro-3-oxobutanoates, the corresponding methyl and methoxyethyl 4-(2-formylaminoethylthio)-3-oxo-bu- tanoates are prepared. Preparation 2 Acetic acid is added to a solution of ethyl 4-(2-formylamino-ethylthio)-3-oxo-butanoate (12.5 g) in MeOH (ml 120), previously saturated with ammonia at 0°C and cooled at 0°C, up to pH 4-45. The mixture is refluxed for 2 hours and the excess solvent is evaporated in vacuum, to give a syrup from which a solid material is separated b treatment with AcOEt. After filtration, the organic phase i washed with water (8x10 ml), and with a saturated aqueou solution of NaHCO (3x10ml), dried (Na SO.) and concentrate in vacuum to give 11,5 g of ethyl 3-amino-4-(2-formylamin ethylthio)crotonate as a yellow oil. 1H-NMR: (CDC1 ) , & ,TMS) :1.10-1.20(3H,t) ; 2.20-2.60 (4H, ) ; 3.80-4.10 (4H, m) ; 5.20-5.40 (2H, m) ; 5.70 (1H, m); 6.60 (1H, m) ; 8.10 (1H, s). Methyl and methoxiethyl 3-amino-4-(2-formylaminoethylthio) crotonate are likewise prepared. Preparation 3 A solution of 3-chlorobenzaldeyde (10 g) , ethyl 4-(2-formylaminoethylthio)-3-oxo-butanoate (16,7 g) , AcOH (2 ml) and piperidine (0,6 ml) in benzene (120 ml) is refluxed in a Dean-Stark apparatus for 6 hours . After cooling at room temperature, the mixture is washed with H O (3x20 ml), with a saturated solution of NaHCO (3x10 ml), with a solution of H S0 4 (2N, 3x10 ml) and again with H O (3x30 ml). The organic phase is dried (Na SO.) and concentrated in vacuum to give 16 g of ethyl 2-Z, E-(3-chlorophenylmethylene)-4-(2- -formylaminoethylthio)-3-oxo-butanoate, as an oil. 1H-NMR(CDC1 ), S (TMS), 1.1-1,2. (3H, t); 2.2-2.4 (2H,t); 2.7-3.0 (2H, m) ; 8.9-4.2 (4H, m) ; 6.8-7.9 (7H, m) . EXAMPLE 1 A solution of ethyl 3-amino-4-(2-formylamino ethylthio) crotonate (5.3 g) and 3-Z, E-(m-nitrophenylmethy- lene) - 2, 4-pentanedione (4.9 g; from Knoevenagel condensation of 3-nitrobenzaldeyde with 2 , 4-pentanedione) in EtOH (100 ml) is refluxed for 4 hours, it is cooled at 0°C and acidified (pH 1:2) with few drops of EtOH saturated with gaseous- HCl. After 15 minutes the solvent is evaporated at reduced pressure, the residue dissolved in AcOEt (80 ml), washed with a saturated solution of NaHCO (3x15 ml) , with water (3x30 ml), dried (Na„SO„) and concentrated. The ' 2 4 residue is purified by chromatography on SiO (300 g, eluent AcOEt/MeOH 80/20) to give 4.9 g of 2-(2-formylaminoethyl- tnio-)" methyl-3-ethoxycarbonyl-4-(m-nitrophenyl) 5-acetyl-6- -methyl-l,4-dihydropyridine, m.p. 140-142°C (EtOH). EXAMPLE 2 A solution of ethyl 3-amino-4-(2-formylaminoethylthio) crotonate (1 g) and methyl 2-Z,E- (2-nitro-5-methylthiophe- nylmethylene)-3-oxobutanoate (0,95 g,rα.p. 69-7, obtained by Knoevena'gel condensation of methyl acetoacetate and 2-nitro-5-methylthiobenzaldehyde) , in EtOH (ml 10) is * refluxed for 24 hours, then it is evaporated to dryness. The residue is dissolved in Et_0 (ml 30), washed with HCl (2N, 3x5 ml) , H O (3x10 ml) , dried and concentrated in vacuum. After column chromatography on SiO 30 g, eluent Et 0/AcOEt 60/40) 1.1 g of 2-(2-formylamino ethylthio)methyl-3-ethoxy- carbonyl-5-methoxyσarbonyl-4-(2-nitro-5-methylthiophenyl)-6- methyl-l,4-dihydropyridine, m.p. 148-150° (Et 0) are obtained. Using the procedure of example 1 or 2 the following compounds are prepared: -2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycarbonyl-4-(2 , 3-dichlorophenyl)-6-methyl-l,4-dihydro- pyridine, m.p. 122-124°C. -2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-cya- no-4-phenyl-6-methyl-l,4-dihydropyridine -2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-cya- no-4-(3-methoxyphenyl)-6-methyl-l,4-dihydropyridine -2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-ben- zoyl-4-phenyl-6-methyl-l, 4-dihydropyridine -2-(2-formylaminoethylthio)methyl-3-methoxycarbonyl-5-metho- xycarbonyl-4-(2-fluoro-3-methythio-phenyl)-6-methyl-l,4-di- hydropyridine -2-(2-formylaminoethylthio)methyl-3-methoxycarbonyl-5-metho- xycarbonyl-4-(2-methylthiophenyl)-6-methyl-l,4-dihydropyri- dine. Example 3 A solution of ethyl 3-amino-4-(2-formyl-amino- ethylthio)crotonate (7 g) and methyl 2-E, Z-(3-nitro-phe- nylmethylen)-3-oxobutanoate (6 g) in ETOH (70 ml) is re¬ fluxed for 18 hours, then it is concentrated in vacuum. The residue is dissolved in AcOEt (100 ml), washed with HCl (2N, 3 x 30 ml), H O (3 x 50 ml), dried on Na SO , concentrated in vacuum and purified by column chromatogra¬ phy on SiO (300 g, eluent AcOEt-hexane 90/10), obtaining 8 g of 2-(2-formylaminoethylthio) -methyl-3-ethoxycarbo- nyl-5-methoxycarbonyl-4-(m-nitrophenyl) -6-methyl-l.4-dihy- dropyridine, m . 109-11°C. Example 4 A solution of ethyl 2-Z,E-(3-chlorophenylmethyle- ne)-4-(2-formylaminoethylthio) -3-oxo-butanoate (4 g) and methyl 3-aminocrotonate (1.2 g) in EtOH (40 ml) is reflu¬ xed for 3 hours, the mixture cooled at room temperature acidified to pH 1:2 with a few drops of EtOH saturated with gaseous Hcl and, after 20 minutes, evaporated to dryness; a solution of the residue in AcOEt (60 ml) is washed with asaturated solution of NaHCO (3 x 10 ml), H O (3 x 20 ml), dried (Na SO ) and concentrated in vacuum. After chromatography on silica gel (150 g; eluent: AcOEt) 4.3 g of 2-(2-formylaminoethylthio)methyl-3-ethoxycarbo- nyl-4-(3-chlorophenyl)-5-methoxycarbonyl-6-methyl-l.4-dhy- dropyridina are obtained as a foam. X H-NMR (CDC1 ) : < f (TMS) = 1.10-1.25 (3H, t) ; 2.20-2.80 (5H, m) ? 3.20-3.70 (5H, m) ; 3.80-4.20 (4H, ) ; 5.00 (1H, s); 6.50 (1H, m) 7.00-7.20 (5H, m) ; 8.10 (1H, s). Using the above described procedure, the following compounds are prepared - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-cyano -4-(m-chlorophenyl)-6-methyl-l.4-dihydropyridine - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-(2-N, N-dimethylamino)ethoxycarbonyl-4-(m-chlorophenyl)-6-methyl- 1.4-dihydropyridine - 2-(2-formylaminoethyltio)methyl-3-ethoxycarbonyl-5-isopro poxycarbonyl-4(m-chlorophenyl)-6-methyl-l.4-dihydropyridine - 2-(2-formylamonoethylthio)methyl-3-ethoxycarbonyl-5-t-bu- toxycarbonyl-4(m-chlorophenyl)-6-methyl-l.4-dihydropyridine - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-metho xycarbonyl-4-(m-nitrophenyl)-6-methyl-l.14-dihydropyridine, p. 109-11°C. Example 5 In nitrogen atmosphere , a stirred mixture of 2-mer- captomethyl-3-ethoxycarbonyl-5-methoxycarbonyl-4 (m-nitrophe nyl ) -6-methyl-l . 4-dihydropyridine ( 5 g ) , -, o - > ( 2 •?) and ( S ) -2- forrαylamino -2-phenyl-ethanolmethanesulfonate ( 3 g from S ( + ) -N-f ormylphenylglycin) in DM A ? ( 50 ml ) is heated for 12 hours at 50°C. The cooled mixture is poured in ice-water (500 ml) and extracted with AcOEt (30 ml x 3). After usual work-up, the combined organic extracted are evaporated to dryness, to give a diasteroisomer mixture of 4(S)2'(S) and 4(R)2 • (S)-2-(2'-formylamino-2'-phenylethyl- thio),methyl-3-ethoxycarbonyl-5-methoxycarbonyl-4-(m- nitrophenyl)-6-methyl-l.4-dihydropyridine, which are separated by HPL-chromatography on SiO (eluent AcOEt/hexane) . The more polar diasteroisomer (1.7 g) is a foam, the less polar is obtained as crystalline compound ( 2.2 g, m.p. 66-70°C, C 27 H 29 N 3 0 7 S • 2 3 Et 2 0) ' Using the above described procedure the following compounds are prepared: - 2-( 2-formylamino-3-phenylpropylthio)methyl-3-ethoxycarbo nyl-5-methoxycarbonyl-4-(m-nitrophenyl)-6-methyl-l.4-dihy- dropyridine; - 2-(2-formylaminopropylthio)methyl-3.5-di-ethoxycarbonyl- 4-(m-trifluoromethylphenyl)-6-methyl-l.4-dihydropyridine; - 2-/2-formylamino-3-( 4-imidazolyl)propylthio/methyl-3-me- thoxycarbonyl-5-isopropoxycarbonyl-4-(m-cianophenyl)-6-me¬ thyl-l.4-dihydropyridine - 2 ' -R-2-(2 ' -formylamino-2 ' -phenylethylthio)methyl-3-etho- xycarbonyl-5-methoxycarbonyl-4-(m-nitrophenyl)-6-methl-l.4 dihydropyridine - 2-( 2-formylaminoethylthio)methyl-3-ethoxycarbony1-5-me¬ thoxycarbony1-4-(m-nitrophenyl-6-methyl-l.4-dihydropyridine m.p.l09-lll°C. Example 6 A solution of 2-chloromethyl-3-ethoxycarbonyl-4- (o-methylthiophenyl)-5-methoxycarbony1-6-methy1-1.4-dihydro pyridine (2.1 g) in EtOH (10 ml) is added dropwise at 0°C to a solution of N-n-butyl-N-(2-acetylthioethyl)formamide (1.2 g) and NaOH (20% water solution, 1.2 g) . After 3 hours at 0°C, the reaction mixture is warmed at room tem¬ perature and stirred for 30 minutes, then it is concentra¬ ted in vacuum. After usual work-up, and column chromato¬ graphy on SiO (80g; eluent AcOEt/Et O 70/90) 1.8 g of 2-/2-(N-formyl-N-butylamino)ethylthio/methyl-3-ethoxycar- bonyl-4-(o-methylthiophenyl)-5-methoxycarbonyl-6-methyl- 1.4-dihydropyridine are obtained as a foam. 1 H-NMR (CDC1 ) : ό s- (TMS) 0.1-1.3 (6H, ) : 1.5-2.0 (4H, m; 2.1-3.0 (10H, m) ; 3.20-4.20 (8H, m) ; 5.10 (1H, s) ; 6.8 (1H, m) ; 6.9-7.4 (6H, m) ; 8.1 (1H, s) Using the above described procedure the following compounds are prepared: - 2- " (N-formyl-pyrrolidin-2-yl)methyl-thioτ 7 methyl-3-etho- xycarbonyl-5-methoxycarbonyl-4-(m-nitrophenyl)-6-methyl- l r 4-dihydropyridine; - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- toxycarbonyl-4-(2-chlorophenyl)-6-methyl-l, -dihydropy- ridine; - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycarbonyl-4-(2-trifluoromethylphenyl)-6-methyl-l, -dihy dropyridine; 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycar- bonyl-4-(3-cyanophenyl) -6-methyl-l, 4-dihydropyridine; - 2-(2-formylaminoethylthio)-3-ethoxycarbonyl-5-metoxycar- bonyl-4-(3-methoxyphenyl)-6-methyl-l, 4-dihydropyridine ; - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycarbonyl-4-phenyl-6-methyl-l, 4-dihydropyridine; - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycarbonyl-4-(p-fluorophenyl)-6-methyl-l, 4-dihydropy- ridine; - 2-( 2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycarbonyl-4-( 2-trifluoromethylphenyl)-6-methyl-l, 4- dihydropyridine; 2-(2-formylaminoethylthio)methyl-3, 5-di-ethoxycarbo- nyl-4- ( 3-pyridyl) -6-methyl-l, 4-dihydropyridine; - 2- (4-formylaminoethylthio)methyl-3, 5-diethoxycarbonyl-4- (m-chlorophenyl)-6-methyl-l, 4-dihydropyridine; - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-me- thoxycarbonyl-4-(m-nitrophenyl) -6-methyl-l, 4-dihydropy- ridine, mp 109-111°C - 2-( 2-formylaminoethylthio)methyl-3-ethoxycarbonyl-4-/ benzo-(2, 3-b)-1, 4-dioxan-o.-yl/-5-methoxycarbonyl-6-me- thyl-1, 4-dihydropyridine; - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5- ( 2- N-methyl-N-benzylamino)ethoxycarbonyl-4-(m-nitrophenyl)-6- methyl-1, 4-dihydropyridine . Example 7 A solution of 2-(2-aminoethylthio)methyl-3-ethoxy- carbony1-5-methoxycarbony1-4- ( 2-chlorophenyl)-6-methyl-l, 4- dihydropyridine (4.4 g) in THF (44 ml) is added to a stirred solution of N-formylimidazole, "in situ" prepared from formic acid (0.6 ml) and N, -carbonyldiimidazole (2.5 g) , at 0°C, in THF (25 ml) . After 30 minutes the reaction mixture is poured into ice/water (2/1; 500 ml), extracted with AcOEt (3 x 50 ml) , the combined extracts washed with a saturated solu¬ tion of NaH PO (3 x 10 ml), a saturated solution of NaHCO (3 x 10 ml), and then with H O (3 x 50 ml), dried (Na ^ SO and concentrated under vacuum. 2 4 3.7 g of 2-(2-formylaminoethylthio)methyl-3-etho- xycarbonyl-5-carbomethoxy-4-(2-chloro)-6-methyl-l,4-di- hydropyridine are obtained from Et 0. Example 8 A solution of 2- 2- N-(2-cyanoethyl)aminoethyl - thic^methyl-3-ethoxycarbonyl-5-methoxycarbony1-4-(m-nitrophe nyl)-6-methyl-l,4-dihydropyridine(5.8 g) in N,N-dimethyl- formamide (100 ml) and formic acid (100 ml) is heated at 100°C for 6 hours, then poured in ice/water (500 ml) extracted with Et 0(3 x 50 ml); the combined organic extracts are washed with a saturated solution of NaHCO (3 x 30 ml), H O (3 x 20 ml), dried (Na SO ) and evaporated to dryness. The residue is crystallized from EtOH to give 4.15 g of 2- 2-N-(2-cyanoethyl)-N-formylaminoethyl- thiq/methyl-3-ethoxycarbonyl-5-methoxycarbonyl-4-(m-nitro¬ phenyl)-6-methyl-l, 4-dihydropyridine, mp 110-112°C. Using the above described procedure, the following compounds are prepared. - 2-(2-formylamino-2-ethoxycarbonylethylthio)methyl-3, 5- diethoxycarbonyl-4-(m-nitrophenyl)-6-methyl-l, 4-dihydro- pyridine - 2-(2-formylaminoethylthio)methyl-3-ethoxycarbonyl-5-nitro -4-(m-nitrophenyl)-6-methyl-l, 4-dihydropyridine. Example 9 A solution of m-chloroperbenzoic acid (1.3 g, 1 equiv. mol) in 1, 2-dichloroethane (15 ml) is added at -10°C to a solution of 2-(2-formylaminoethylthio)methyl-3- -ethoxycarbonyl-5-methoxycarbonyl-4-(m-nitro-phenyl)-6-me¬ thyl-l, 4-dihydropyridine (3.5 g) in 1, 2-dichloroethane (30 ml) . After 30 minutes the solution is filtered, washed with Na S 0 (5% water solution, 3 x 5 ml), NaHCO (satu¬ rated water solution, 3 x 10 ml), H O (3 x 10 ml), dried (Na SO ) and evaporated in vacuum to give 3.4 g of 2-(2-formylaminoethylsulfinyl)methyl-3 -ethoxycar ony1-5- methoxycarbonyl-4-(m-nitrophenyl)-6-methyl-l, 4-dihydropy- ridine as a foam. 1H-NMR (CDC1 S (TMS) : 1.00-1.2 (3H, t); 2.1 (3H, t); 2.80-3.70 (4H, m) ; 3.90-4.30 (4H, m) ; 5.10 (1H, m); 6.50 (1H, m) ; 7.10-8.20 (6H, m) . Using the above described procedure the following 2-(2-formylaminoethylsulfinyl)-6-methyl-l, 4-dihydropyri- dines are prepared: -3-ethoxycarbonyl-5-cyano-4-(m-nitrophenyl) ; -3-ethoxycarbonyl-5-cyano-4-(m-chlorophenyl) ; -3 , 5-diethoxycarbonyl-4-(m-methylthiophenyl) ; -3 , 5-dimethoxycarbonyl-4-(m-trifluoromethylphenyl) . Example 10 A solution of m-chloroperbenzoic acid (3.8 g, equiv. mol) in MeOH (30 ml) is added at 10°C to a solution of 2-( 2-aminoethylthio)methyl-3-ethoxycarbonyl-5- methoxycarbony1-4-(m-nitrophenyl) -6-methyl-l, 4-dihydropy- ridine (5 g) in MeOH (100 ml), the mixture is then warmed at 15-20°C and stirred for 30 minutes. The solvent is then evaporated at reduced pressure, the residue partitioned between CH Cl (80 ml) and H O (30 ml), the organic phase is washed with sodium thiosulfate (5% water solution) and then worked-up as described in example 8 to give 4.8 g of 2-(2-aminoethyl-sulfonyl)methyl-3-ethoxycarbonyl-5-metho- xycarbonyl-4-(m-nitrophenyl)-6-methyl-l, 4-dihydropyridine as an amorphous solid. 1H-NMR (CDC1 ) £ (TMS) 1.0-1.2 (3H, t) ; 2.1 (3H, t); 3.0-3.7 (4H, m) , 4.10-4.30 (4H, m) ; 5.1 (1H, m) ; 6.8 (1H, m) 7.10-8.20 (6H r m) . Using the above described procedure the following compounds are prepared: 2-(2-formylaminoethylsulfonyl)-6-methyl-l,4-dihydropyri- dines: - 3,5-diethoxycarbonyl-4-(m-methylthiophenyl) - 3-ethoxycarbonyl-5-methoxyethoxycarbonyl-4-(m-chlorophe¬ nyl). Example 11 Fractional crystallization of the (+)2-(2-ammonium- methylthio)methyl-3-ethoxycarbonyl-5-methoxycarbonyl-4-(m- nitrophenyl)-6-methyl-l, 4-dihydropyridine salt with (+) and (-)camphor-lθ-sulfonic acids from EtOH produces 2 optical isomers: (+)free base f^J = +1.8 (MeOH c = 10%) as (+)emifumarate salt m.p. 105-107°C, f^J = +3.6 (MeOH c = 9.7%) and (-)free base, f<^J Ω = -1-7 (MeOH c = 19.8%), as emifumarate salt m.p. 106-108°C, ^J = 3.4 (MeOH c = 9.4%) . Starting from the pure enantiomers, as free bases, using the procedures described in examples 7, 8, the enan- tiomeric 2- ( 2-formylaminomethylthio)methyl-3-ethoxycar- bonyl-5-methoxycarbonyl-4-(m-nitrophenyl)-6-methyl-l,4-di- hydropyridines are obtained as amorphous solids. (+)N-formylamino isomer: C H N O S. h H O P*J n = +13.6, f°<-J CAC = +22.2 (MeOH, c = 2.14) D 546 (-)N-formylamino isomer: C H N O S. h H O / " ~ n = -13.7, ^7 CΛC = -22.3 (MeOH, c = 2.07) U j4b

Claims: CLAIMS Compounds of formula I wherein: - R.. represents: - . a COCH COC H CN or NO group; - . a COORa group, wherein Ra represent hydrogen, C -C straight or branched alkyl, optionally bearing one or more C..-C,. alkoxy crroups and/or secondary amino groups of 1 6 formula -NR.R_ wherein R. and R_, that may be the same or 4 5 4 5' J different, represent C.-C,. alkyl, phenyl, benzyl or taken, b together with the nitrogen atom, form a five or six membered ring optionally containing other atoms; - R- is a phenyl ring unsubstituted or substituted with one or more C..-C.. alkyl, halogen, nitro, cyano, C.-C fi alkoxycarbonyl, C -C alkylthio, C.-C. alkylsulfinyl; 6 1 6 pentafluorophenyl; c( - or β-naphtyl; a five- or six-membered heterocyclic ring; o -benzo[2.3-b]-1.4- -dioxan- c -yl; ex.-benzofuroxanyl; - R_ represents a COORa group wherein Ra is as above defined; - P is selected from the group consisting of hydrogen, -(CH_) -W and C. -C_ linear or branched alkyl; 2 p 1 8 ' - N-P is the residue of a primary or secondary amino group wherein P is selected from the group consisting of hydrogen, C -C lower linear or branched alkyl group an -(CH 2 )p- ; - P, taken together with P.. and the nitrogen atom to whic P. is linked, may form a pyrrolidine or a piperidin ring; - W is selected from the group consisting of hydroxymethyl, ormyloxymethyl, CO_R wherein R is hydrogen or C.-C. lower alkyl, CN, saturated or unsaturated heterocyclic ring, C -C_ cycloalkyl ring, phenyl ring optionally substituted by one or more halogens or C.-C -alkoxy groups; - m is an integer from 1 to 3 ; - n is zero or an integer from 1 to 2; - p is zero or an integer from 1 to 3 their salts, enantiomers, diastereoisomers or mixtures thereof. 2. Compounds according to claim 1 wherein R represents a COCH_, COC.H_, CN or NO_ group. 3 6 5 2 • Compounds according to claim 1 wherein R. represents a COORa group, wherein Ra is as above defined. 4. Compounds according to any one of the previous claims wherein R is a phenyl ring unsubstituted or substituted with one or more <Z -C alkoxy carbonyl, C.-C,. alkylthio, 1 σ 1 6 C 1 -C fi alkylsulfinyl. 5 ' . A compounds according to claim 1 selected in the group of: a 4-(3-nitrophenyl) , (3-chlorophenyl) , (3-cyanophenyl) , (3-methoxyphenyl) , (4-fluorophenyl) , (3-methylthiophenyl) , (o.-benzo[2,3-bj -1.4 -dioxan-oc -yl) , (2-fluo- ro-5-methylthiophenyl)- 2-(2-formylaminoethyl¬ thio)methyl-3-ethoxycarbonyl-5-methoxycarbonyl-6-me- thyl- 1,4-dihydropyridine. a diastereoisomer of 2-(2-formylamino-2-phenylethylthio) methyl-3-ethoxycarbonyl-5-methoxy carbonyl-4-(m-nitro¬ phenyl)-6-methyl-l,4-dihydropyridine. a diastereoisomer of 2-(N-formyl-pyrrolidin-2-yl methyl- thio)methyl-3-ethoxycarbonyl-5—methoxycarbonyl-4- -(m-nitrophenyl)-6-methyl-l.4-dihydropyridine. - 2-(2-formylaminoethylthio) methyl-3-ethoxycarbonyl-5- -(2-N,N-dimethylamino)ethoxycarbonyl-4-(m-chlorophe¬ nyl)-6-methyl-l,4-dihydropyridine. - 2-(2-formylaminoethylthio)methyl-3-methoxyethoxycarbo- nyl-5-methoxycarbonyl-4-(3-methylthiophenyl)-6-methyl— -1, 4-dihydropyridine. - 2-[2-N-{2-cyanoethyl)-N-formylaminoethylthio]methyl- -3-ethoxycarbonyl-5-methoxycarbonyl-4-(m-nitrophenyl)- -6-methyl-l,4-dihydropyridine. - 2-(2- ormylaminoethylthio)methyl-3-ethoxycarbonyl-5- -(2-N-methyl-N-benzylamino)ethoxycarbonyl-4-(m-nitro¬ phenyl)-6-methyl-l,4-dihydropyridine. 6 . A process for the preparation of a compound of formula I comprising one of the following steps: a) forming a thioether bond by reaction of a compound of general formula (II) with a compound of formula III wherein: R 1 , R„, R m, P, N-P are as above defined; - either y or Y' represents a thiol or a masked thiol group such: as- thio C -C. _ alkanoyl ester or a thiouronium salt -S C=NR_)NR_R 0 y the other being a known leaving 6 7 o 2 group such as chlorine, bromine, iodine, trifluoromethane sulphonate, an alkyl- or an arylsulphonate; - R_, R_, R 0 , which are the same or different, are hydrogen or a C. -C alkyl group and y (-) is a pharmaceutically acceptable anion; b) cyclizing a compound of formula IV H with an alkylidene R 3 ι cxNH 2 < IV > ompound of formula V wherein: R R R P, NP m, are as above defined. c) cyclizing a compound of formula VI with an alkylidene compound of formula VII wherein R R P, NP and m are as above defined and R. , is prefarably CO„ 2Ra, CN, COCHJ_, COC6_H5_. d) formylating a compound of formula VIII wherein R. , R R P, P.. and n are as above defined, the products obtained by said reaction a) -d) being optionally subjected to oxydation, esterification, separation of isomers. 7. Pharmaceutical compositions containing a compound of claims 1-6 in admixture with a pharmaceutically acceptable excipient.

Inventors: FRIGERIO MARCO, GANDOLFI CARMELO A, SPINELLI SILVANO, TOFANETTI ODOARDO, TOGNELLA SERGIO, FRIGERIO, MARCO, GANDOLFI, CARMELO, A., SPINELLI, SILVANO, TOFANETTI, ODOARDO, TOGNELLA, SERGIO
Applicants: BOEHRINGER BIOCHEMIA SRL, BOEHRINGER BIOCHEMIA ROBIN S.P.A.
Application year: 1987
Main classification: C07D211
IPC codes: C07D211, C07D401, C07D405, C07D409, C07D413, C07D417, C07D521