NOVEL SUBSTANCES DC-92B AND DC-92D, AND PROCESS FOR THEIR PREPARATION

Abstract: Anthra(1,2-b)pyran antibiotics of formula (I) are new. R is (II) (antibiotic DC-92B) or (III) (antibiotic DC-92D). Pref. prepn. comprises a culture of a strain of Actinomadura (esp. Actinomadura sp. DO-92, FERM-P 1019). Pref. the antibiotics are dark red solids, soluble in (m)ethanol, acetone, chloroform, ethylacetate, toluene and DMSO but insol. in water and hexane. IR, (1)H NMR and (13)C NMR spectral data are given.

Description: Technical Field The present invention relates to anti-tumor antibiotics DC-92B and DC-92D having the anthraquinone moiety and to a process for producing the same. Background Art Mitomycins, anthracyclines, anthraquinones, etc. are known as anti-tumor antibiotics having the quinone moiety [CRC Handbook of Antibiotic Compounds, Volume 3, 1981 (CRC Press U.S.A.)]. Of these, the compounds represented by the following formula are illustrative of anthraquinone compounds. R A R B Pluramycin A COCH₃ X Kidamycin H Z Hedamycin H Y Neopluramycin COCH₃ Z Mitomycin C, adriamycin, bleomycin and others are known as anti-tumor antibiotics used for chemotherapy against cancers. However, there has been a constant demand for substances with higher anti-tumor activity because these anti-tumor substances presently available are ineffective against some types of cancer and involve the problem of drug resistance. Disclosure of the Invention In accordance with the present invention, novel substances DC-92B and/or DC-92D having anti-tumor and antibacterial activities can be obtained by culturing a microorganism belonging to the genus Actinomadura and capable of producing DC-92B and/or DC-92D. The physicochemical properties of DC-92B are shown below. (a) Molecular weight: 776 (Mass spectrometry; SI-MS m/z 777 [M+H]⁺) (b) Color: Dark red (c) Ultraviolet absorption spectra: As shown in Figures 1 and 2 [Figure 1: in methanol, Figure 2: in alkaline (NaOH) methanol] (d) Infrared absorption spectrum: As shown in Figure 5 (measured in CHCl₃) (e) Solubility: Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate, toluene and dimethylsulfoxide; sparingly soluble in water and hexane (f) ¹H-NMR spectrum (400 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 13.88 (1H, br s), ca.9.7 (1H, br), 8.34 (1H, br s), 8.04 (1H, d, J=0.6 Hz), 6.53 (1H, s), 5.50 (1H, br d, J=11.0 Hz), 5.45 (1H, br dd, J=10.2, 1.4 Hz), 3.56 (1H, dq, J=8.6, 6.0 Hz), 3.54 (1H, s), 3.22 (1H, br dd, J=9.8, 8.6 Hz), 3.17 (1H, d, J=4.9 Hz), 3.02 (3H, d, J=0.6 Hz), 2.86 (1H, ddd, J=13.2, 9.8, 3.4 Hz), 2.72 (1H, d, J=4.9 Hz), 2.61 (6H, br s), ca.2.6 (1H), 2.32 (6H, s), 2.19 (1H, m), 1.91 (3H, s), 1.59 (3H, s), 1.44 (3H, d, J=6.0 Hz), 1.38 (1H, br dd, J=11.1, 3.8 Hz), 1.35 (3H, s), 1.12 (3H, s), ca.1.1 (1H, m), 1.08 (3H, s) (g) ¹³C-NMR spectrum (100 MHz measured in CDCl₃, internal standard: TMS) δ(ppm): 187.8, 183.2, 178.3, 164.9, 159.3, 156.0, 150.0, 140.0, 138.8, 137.5, 132.6, 126.2, 126.1, 126.0, 119.1, 115.6, 110.9, 100.0, 79.0, 77.5, 75.2, 71.7, 67.3, 63.6, 62.9, 59.9, 59.0, 58.6, 58.2, 43.1, 41.0, 40.4, 28.5, 25.2, 24.2, 24.1, 19.9, 19.7, 18.8, 14.3 (h) Thin layer chromatography: R f = 0.54 [Chemically bonded silica gel thin layer (HPTLC plate Art 15647, E. Merck), developer: toluene/acetone (6:4 V/V)] (i) Weakly basic substance The physicochemical properties of DC-92D are shown below. (a) Molecular weight: 774 (Mass specrometry; SI-MS m/z 775 [M+H]⁺) (b) Color: Dark red (c) Ultraviolet absorption spectra: As shown in Figures 3 and 4 [Figure 3: in methanol, Figure 4: in alkaline (NaOH) methanol] (d) Infrared absorption spectrum: As shown in Figure 6 (measured in CHCl₃) (e) Solubility: Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate, toluene and dimethylsulfoxide; sparingly soluble in water and hexane (f) ¹H-NMR spectrum (400 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 13.68 (1H, br), ca.9.65 (1H, br), 8.02 (1H, br s), 7.84 (1H, d, J=0.5 Hz), 6.54 (1H, s), 5.46 (1H, br d, J=11.0 Hz), 4.97 (1H, d, J=2.1 Hz), 4.33 (1H, dq, J=9.5, 6.3 Hz), 3.66 (1H, dd, J=9.5, 8.4 Hz), 3.49 (1H, dd, J=8.4, 2.1 Hz), 3.48 (1H, s), 3.17 (1H, d, J=5.0 Hz), 3.02 (3H, d, J=0.5 Hz), 2.74 (1H, d, J=5.0 Hz), ca.2.6 (1H), 2.60 (6H, br s), 2.42 (6H, s), 1.91 (3H, s), 1.55 (3H, s), 1.42 (3H, d, J=6.3 Hz), 1.34 (3H, s), 1.33 (1H, dd, J=14.7, 11.5 Hz), 1.11 (3H, s), 1.09 (3H, s) (g) ¹³C-NMR spectrum (100 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 188.0, 181.0, 178.3, 164.9, 159.8, 156.5, 156.1, 150.3, 138.7, 137.3, 136.4, 129.4, 128.8, 126.1 (2), 119.0, 116.3, 111.0, 100.0, 95.5, 79.0, 76.7, 69.2, 66.1, 63.6, 62.6, 59.8, 59.0, 58.6, 58.2, 43.3, 41.0, 40.9, 25.2, 24.25, 24.16, 19.8, 19.7, 17.7, 14.4 (h) Thin layer chromatoyraphy: R f = 0.20 [Chemically bonded silica gel thin layer (HPTLC plate Art 15647, E. Merck), developer: toluene/acetone (6:4 V/V)] (i) Weakly basic substance Planar structural formulas of DC-92B and DC-92D were determined as shown below based on the physicochemical properties given above in items (a), (c), (d), (f), (g), etc. Biological properties of DC-92B and DC-92D are shown below. (A) Minimum inhibitory concentration against various bacteria (MIC: µg/mℓ) Bacteria tested DC-92B DC-92D Staphylococcus aureus ATCC 6538P 0.04 1.5 Bacillus subtilis No. 10707 0.15 3.0 Klebsiella pneumoniae ATCC 10031 2.5 25 Escherichia coli ATCC 26 10 100 Shigella sonnei ATCC 9290 10 100 Salmonella typhosa ATCC 9992 40 100 The antibacterial activity was evaluated by the agar dilution method using a medium (pH 7) prepared by dissolving 3 g of Bacto-Tryptone (Difco Laboratories), 3 g of meat extract, 1 g of yeast extract, 1 g of glucose and 16 g of agar in 1 liter of water. (B) Acute toxicity The acute toxicity values (LD₅₀) of DC-92B and DC-92D were about 0.31 mg/kg and about 5.6 mg/kg, respectively, by intraperitoneal administration to mice. (C) Anti-tumor activity (1) Therapeutic effect against Sarcoma 180 tumor Six male ddY-strain mice each having a weight of about 20 g were used for each group as test animals, and 5 x 10⁶ cells of Sarcoma 180 ascites tumor were implanted subcutaneously into the animals at the axilla. 0.2 mℓ of phosphate buffered saline (hereinafter referred to as PBS) containing DC-92B or DC-92D at various concentrations was administered once intraperitoneally 24 hours after implantation. The composition of PBS was 0.8 g/dℓ NaCl, 0.02 g/dℓ KCl, 1.15 g/dℓ Na₂HPO₄ and 0.02 g/dℓ KH₂PO₄ (pH 7.2). For comparison, 0.2 mℓ of PBS containing mitomycin C was intraperitoneally administered to a group of animals 24 hours after tumor implantation. The table below shows the average tumor volume (mm³) and T/C (T: average tumor volume of the test groups, C: average tumor volume of the control group which received an intraperitoneal administration of 0.2 mℓ of PBS) ten days after implantation. Compound Dose (mg/kg) Average tumor volume (mm³) T/C DC-92B 0.31 828.6 0.39 DC-92D 6.25 764.9 0.36 3.125 679.9 0.32 1.563 1444.8 0.68 Mitomycin C 6.0 743.8 0.35 Control - 2124.7 - (2) Therapeutic effect against lymphocytic leukemia P388 tumor Five male CDFl mice each having a weight of about 22 g were used for each group as test animals, and 1 x 10⁶ cells of lymphocytic leukemia P388 tumor were implanted intraperitoneally into the test animals. 0.2 mℓ of PBS containing DC-92B or DC-92D was administered once intraperitoneally 24 hours after implantation. For comparison, 0.2 mℓ of PBS containing mitomycin C was intraperitoneally administered to a group of animals 24 hours after tumor implantation. The table below shows the mean survival days after implantation and T/C (T: mean survival days for the test groups, C: mean survival days for the control group). Compound Dose (mg/kg) Survival days Increased Life Span (T/C) DC-92B 0 (control) 11.0 - 0.15 16.2 1.47 0.025 15.5 1.41 0.0125 15.5 1.41 DC-92D 3.13 7.6 0.69 1.56 12.7 1.15 0.78 12.9 1.17 0.39 11.4 1.04 0.75 17.4 1.58 Mitomycin C 6.0 17.9 1.63 The process for producing DC-92B and DC-92D is described below. DC-92B and/or DC-92D can be obtained by culturing a DC-92B and/or DC-92D-producing strain belonging to the genus Actinomadura in a nutrient medium until DC-92B and/or DC-92D are accumulated in the culture, and recovering DC-92B and/or DC-92D from the culture. Any strains of the genus Actinomadura capable of producing DC-92B and/or DC-92D may be used as the DC-92B and/or DC-92D-producing strains. Some mutant strains derived therefrom through artificial mutation means such as ultraviolet irradiation have been found to be capable of producing DC-92B and/or DC-92D. A typical example is DO-92 strain. Microbiological properties of DO-92 strain are shown below. (1) Morphology Aerial mycelium : Branched but not fragmented Substrate mycelium : Not fragmented Spore : Spores in short flexuous chains develop at the end of simply branched aerial mycelium. Surface of spore : Smooth Shape and size of spore : Elliptical, about 0.6 x 1.1 µm Motility of spore : No movement observed Formation of sclerotium and sporangium is not observed. (2) Color Aerial mycelium : Beige Substrate mycelium : Beige to yellowish brown Soluble pigment : Cream to yellowish brown Melanin pigment : Not formed (3) Chemical properties Steric configuration of diaminopimelic acid: meso type Whole cell sugar : A trace of madurose is present. (4) Cultural characteristics on various culture media Cultural characteristics of DO-92 strain on various media observed after culturing at 28°C for three weeks are shown in Table 1. Color was represented in accordance with Color Harmony Manual (Container Corporation of America). In Table 1, G represents growth, AM the degree of formation of aerial mycelia and their color, SM the color of substrate mycelia, and P the formation of soluble pigment and its color. (5) Physiological properties Growth temperature range was determined after 2 days of culturing. The action on defatted milk and cellulose was observed after 1 month of culturing at 28°C. The other observations were made after 2 weeks of culturing at 28°C. Growth temperature range : 20 to 37°C Optimum growth temperature range : 28 to 32°C Liquefaction of gelatin : Negative Decomposition of cellulose : Negative Coagulation of defatted milk : Negative Peptonization of defatted milk : Positive Formation of melanin pigment : Negative (6) Assimilation of carbon sources Carbon source Growth Carbon source Growth D-Glucose + D-Fructose + L-Arabinose ± L-Rhamnose + D-Xylose ± Sucrose ± Inositol ± Raffinose ± D-Mannitol ± (+: Good growth, ±: Very poor growth) (7) Identification of DO-92 strain DO-92 strain belongs to the Type III cell wall group and to the Type B whole cell sugar group according to the classification of Lechevalier, et al . (Int. J. Syst. Bacteriol., 20 , 435-443, 1970), since it contains mesodiaminopimelic acid in its cell walls, and madurose is detected as a characteristic sugar among whole cell sugars. Morphological characteristics such as formation of aerial mycelia simply branched and formation of short spore chains at the end thereof indicate that this strain belongs to the genus Actinomadura in Actinomycetales. Therefore, DO-92 strain was identified as a strain of Actinomadura , named Actinomadura sp. DO-92, and deposited with the Fermentation Research Institute, Agency of Industrial Science and Technology under FERM BP-1019 on April 23, 1986. For the culturing in the present invention, conventional methods for culturing Actinomycetes are generally used. Any culture media may be employed which contain carbon sources, nitrogen sources, inorganic substances and other nutrients in proper amounts. As the carbon source, glucose, starch, glycerol, mannose, fructose, sucrose, molasses, etc. can be used either alone or in combination. Hydrocarbons, alcohols, organic acids, etc. may also be used depending upon the assimilation ability the strain employed. As the nitrogen source, organic and inorganic nitrogen-containing compounds such as ammonium chloride, ammonium sulfate, ammonium nitrate, sodium nitrate and urea, and natural nitrogenous substances such as peptone, meat extract, yeast extract, dry yeast, corn steep liquor (CSL), soybean meal and Casamino acid can be used either alone or in combination. In addition, inorganic salts such as sodium chloride, potassium chloride, ferrous sulfate, zinc sulfate, manganese sulfate, copper sulfate, nickel sulfate, calcium carbonate and phosphates are added at need. Organic or inorganic substances which promote the production of DC-92B and/or DC-92D such as biotin and vitamins may also be added. Culturing is preferably carried out by liquid culture, and most preferably by submerged stirring culture. Culturing temperature is 20 to 37°C, preferably 28 to 32°C. It is desirable to maintain the pH of the medium at 4 to 10, preferably 6 to 8 by adding aqueous ammonia or aqueous ammonium carbonate during the culturing. Usually, by liquid culture for 1 to 7 days, the desired substances are produced and accumulated in the culture. Culturing is discontinued when the amount of the products in the culture reaches the maximum. For the isolation and purification of DC-92B and/or DC-92D from the culture, an ordinary method for isolating a microbial metabolite from the culture can be utilized. For example, the culture is subjected to filtration, centrifugation, or the like to remove microbial cells, and the resulting filtrate or supernatant is passed through a column packed with a non-ionic porous resin such as HP-20 (manufactured by Mitsubishi Chemical Industries Ltd.) to adsorb the active component. Then, the adsorbed active component is eluted with methanol. The eluate is concentrated to dryness, and the residue is extracted with ethyl acetate. The extract is again concentrated to dryness, and the residue is purified using gel filtering aids, silica gel (Wako Gel C-200; Wako Pure Chemical Industries, Ltd.), etc. to give a crude product of DC-92B and/or DC-92D. The product is further purified by treatment with silica gel (Wako Gel L.C NH₂; Wako Pure Chemical Industries, Ltd.), etc. Purity of the obtained DC-92B and/or DC-92D can be further raised by recrystallization, HPLC and other purification techniques. During the cultivation and purification steps, DC-92B and/or DC-92D can be traced by bioassay using Bacillus subtilis No. 10707, or by measuring ultraviolet absorption of thin layer chromatograms. DC-92B and DC-92D can be used as anti-tumor agents in suitable dosage forms prepared by combination with at least one pharmaceutical diluent, adjuvant or carrier. For example, DC-92B and DC-92D are usually dissolved in physiological saline, glucose solution, lactose solution or mannitol solution to prepare injections and administered intravenously to mammals, particularly human beings, respectively in doses of 0.002-0.22 mg/kg and 0.04-4 mg/kg. They may also be administered intra-arterially, intraperitoneally or intrathoracically in similar doses. Freeze-drying according to the method specified in Pharmacopoeia of Japan may be applied to them, and injectable powder can be prepared by adding sodium chloride to freeze-dried DC-92B or DC-92D. The pharmaceutical preparations of these compounds may also contain pharmaceutically acceptable well-known diluents, adjuvants and/or carriers such as pharmaceutically acceptable salts. When the compounds are used as an injection, it is preferable, in some cases, to use an additive that enhances the solubility of the compounds. Doses may be adjusted appropriately depending on the age and conditions of patients. Administration schedule may also be adjusted depending on the dose, as well as the conditions of patients; intermittent administration, for example, once a week or once every three weeks, may be adopted. DC-92B and DC-92D may also be orally or rectally administered in similar doses and in the similar manner. They are used, with appropriate adjuvants in the form of tablets, powder, granules, or syrup for oral administration and in the form of suppository for rectal administration. The anti-tumor agents thus prepared are expected to be useful for the therapy against chronic lymphocytic leukemia, chronic myeloid leukemia, breast cancer, stomach cancer, hepatoma, colon cancer, rectal carcinoma, lung cancer, pancreatic cancer, uterine carcinoma, cephalic and cervical tumors, etc. The suitable content of DC-92B or DC-92D in the above anti-tumor agents is 0.01 to 20 mg in 20 to 50 mℓ in the case of injection and 0.001 to 85 weight % when they are used in the form of tablets, capsules, powder, granules and suppositories. Brief Description of the Drawings Figure 1 shows the ultraviolet absorption spectrum of DC-92B in methanol. Figure 2 shows the ultraviolet absorption spectrum of DC-92B in alkaline (sodium hydroxide) methanol. Figure 3 shows the ultraviolet absorption spectrum of DC-92D in methanol. Figure 4 shows the ultraviolet absorption spectrum of DC-92D in alkaline (sodium hydroxide) methanol. Figure 5 shows the infrared absorption spectrum of DC-92B (measured in CHCl₃). Figure 6 shows the infrared absorption spectrum of DC-92D (measured in CHCl₃). Example An Example and Reference Examples of the present invention are shown below. Example Actinomadura sp. DO-92 was used as the seed strain. The strain was inoculated into 10 mℓ of a seed medium (pH 7.2, prior to sterilization) containing 10 g/ℓ soluble starch, 5 g/ℓ Bacto-Tryptone (Difco Laboratories), 5 g/ℓ yeast extract, 3 g/ℓ, meat extract, 10 g/ℓ glucose and 2 g/ℓ calcium carbonate in a 50 mℓ-test tube, and incubated at 28°C for 48 hours with shaking (200 rpm). The seed culture thus obtained was inoculated into 300 mℓ of a medium having the same composition as above in a 2 ℓ-flask, and subjected to shaking culture at 28°C for 24 hours. The resulting seed culture was then inoculated into 15 ℓ of a fermentation medium having the following composition in a 30 ℓ-jar fermentor, and cultured with agitation and aeration (rotation: 250 rpm, aeration: 15 ℓ/min.) at 28°C. Composition of the fermentation medium: 15 g/ℓ glucose, 20 g/ℓ cottonseed meal, 0.5 g/ℓ KH₂PO₄ and 0.5 g/ℓ MgSO₄·7H₂O (pH 7.0; adjusted with NaOH prior to sterilization) Culturing was carried out for 90 hours, during which the pH of the medium was adjusted to 6.5 - 7.5 with aqueous ammonia. The microbial cells were separated from the culture by filtration, and the obtained filtrate (15 ℓ) was passed through a column packed with 2 ℓ of a non-ionic porous resin (HP-20; Mitsubishi Chemical Industries Ltd.) to adsorb the active components. The column was washed with water and then with 50% methanol to remove impurities. Elution was carried out with methanol, and the eluate was concentrated, adjusted to pH 10 and extracted with ethyl acetate. The ethyl acetate layer was concentrated, and the concentrate was passed through a column of a molecular sieve, Sephadex LH20 (Pharmacia Fine Chemicals Inc.), followed by elution with methanol to obtain active fractions. The active fractions were then subjected to column chromatography using chemically bonded silica gel (aminopropylsilane-bonded silica gel 40 µm; Baker Inc., USA). Elution was carried out with a 7:3 mixture of toluene and acetone to obtain crude products of DC-92B and DC-92D. The products were subjected to column chromatography using chemically modified silica gel (Wako Gel L.C NH₂; Wako Pure Chemical Industries, Ltd.) and eluted with a 7:3 mixture of toluene and acetone. The active fractions were concentrated to dryness, whereby 15 mg of DC-92B and 7 mg of DC-92D were obtained. These compounds showed the physicochemical and biological properties described hereinbefore. During the cultivation and purification steps, DC-92B and DC-92D were traced by bioassay using Bacillus subtilis No. 10707, and by measuring ultraviolet absorption of thin layer chromatograms. Reference Example 1 (Injection) DC-92B (4 g) was dissolved in 20 ℓ of ethanol, and the solution was filtered under pressure through a Millipore Filter (pore size: 0.22 µ) for sterilization. The germ-free filtrate thus obtained was put in brown-colored vials (5.0 mℓ in each), and freeze-dried by a conventional method to obtain freeze-dried injectable powder (0.2 mg/vial). Reference Example 2 (Tablets) Tablets were prepared by a conventional method, each tablet consisting of 130 mg of DC-92D, 90 mg of lactose, 40 mg of corn starch, 4 mg of polyvinyl alcohol, 28 mg of Avicel and 1 mg of magnesium stearate.

Claims: Compounds represented by the following planar structural formula (I): wherein R is a group represented by formula (II) or formula (III): compound of formula (I) being called DC-92B when R is a group represented by formula (II), and called DC-92D when R is a group represented by formula (III). A compound according to claim 1, which is substance DC-92B characterized by the following physicochemical properties: (a) Molecular weight: 776 (Mass spectrometry; SI-MS m/z 777 [M+H]⁺) (b) Color: Dark red (c) Ultraviolet absorption spectra: As shown in Figures 1 and 2 [Figure 1: in methanol, Figure 2: in alkaline (NaOH) methanol] (d) Infrared absorption spectrum: As shown in Figure 5 (measured in CHCl₃) (e) Solubility: Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate, toluene and dimethylsulfoxide; sparingly soluble in water and hexane (f) ¹H-NMR spectrum (400 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 13.88 (1H, br s), ca.9.7 (1H, br), 8.34 (1H, br s), 8.04 (1H, d, J=0.6 Hz), 6.53 (1H, s), 5.50 (1H, br d, J=11.0 Hz), 5.45 (1H, br dd, J=10.2, 1.4 Hz), 3.56 (1H, dq, J=8.6, 6.0 Hz), 3.54 (1H, s), 3.22 (1H, br dd, J=9.8, 8.6 Hz), 3.17 (1H, d, J=4.9 Hz), 3.02 (3H, d, J=0.6 Hz), 2.86 (1H, ddd, J=13.2, 9.8, 3.4 Hz), 2.72 (1H, d, J=4.9 Hz), 2.61 (6H, br s), ca.2.6 (1H), 2.32 (6H, s) 2.19 (1H, m), 1.91 (3H, s), 1.59 (3H, s), 1.44 (3H, d, J=6.0 Hz), 1.38 (1H, br dd, J=11.1, 3.8 Hz), 1.35 (3H, s), 1.12 (3H, s), ca.1.1 (1H, m), 1.08 (3H, s) (g) ¹³C-NMR spectrum (100 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 187.8, 183.2, 178.3, 164.9, 159.3, 156.0, 150.0, 140.0, 138.8, 137.5, 132.6, 126.2, 126.1, 126.0, 119.1, 115.6, 110.9, 100.0, 79.0, 77.5, 75.2, 71.7, 67.3, 63.6, 62.9, 59.9, 59.0, 58.6, 58.2, 43.1, 41.0, 40.4, 28.5, 25.2, 24.2, 24.1, 19.9, 19.7, 18.8, 14.3 A compound according to claim 1, which is substance DC-92D characterized by the following physicochemical properties: (a) Molecular weight: 774 (Mass spectrometry; SI-MS m/z 775 [M+H]⁺) (b) Color: Dark red (c) Ultraviolet absorption spectra: As shown in Figures 3 and 4 [Figure 3: in methanol, Figure 4: in alkaline (NaOH) methanol] (d) Infrared absorption spectrum: As shown in Figure 6 (measured in CHCl₃) (e) Solubility: Soluble in methanol, ethanol, acetone, chloroform, ethyl acetate, toluene and dimethylsulfoxide; sparingly soluble in water and hexane (f) ¹H-NMR spectrum (400 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 13.68 (1H, br), ca.9.65 (1H, br), 8.02 (1H, br s), 7.84 (1H, d, J=0.5 Hz), 6.54 (1H, s), 5.46 (1H, br d, J=11.0 Hz), 4.97 (1H, d, J=2.1 Hz), 4.33 (1H, dq, J=9.5, 6.3 Hz), 3.66 (1H, dd, J=9.5, 8.4 Hz), 3.49 (1H, dd, J=8.4, 2.1 Hz), 3.48 (1H, s), 3.17 (1H, d, J=5.0 Hz), 3.02 (3H, d, J=0.5 Hz), 2.74 (1H, d, J=5.0 Hz), ca.2.6 (1H), 2.60 (6H, br s), 2.42 (6H, s), 1.91 (3H, s), 1.55 (3H, s), 1.42 (3H, d, J=6.3 Hz), 1.34 (3H, s), 1.33 (1H, dd, J=14.7, 11.5 Hz), 1.11 (3H, s), 1.09 (3H, S) (g) ¹³C-NMR spectrum (100 MHz, measured in CDCl₃, internal standard: TMS) δ(ppm): 188.0, 181.0, 178.3, 164.9, 159.8, 156.5, 156.1, 150.3, 138.7, 137.3, 136.4, 129.4, 128.8, 126.1 (2), 119.0, 116.3, 111.0, 100.0, 95.5, 79.0, 76.7, 69.2, 66.1, 63.6, 62.6, 59.8, 59.0, 58.6, 58.2, 43.3, 41.0, 40.9, 25.2, 24.25, 24.16, 19.8, 19.7, 17.7, 14.4 A process for producing DC-92B and/or DC-92D which comprises culturing a microorganism belonging to the genus Actinomadura and capable of producing DC-92B and/or DC-92 D in a nutrient medium until the compounds are accumulated in the culture, and recovering the compounds from the culture. Compound or compounds according to any of claims 1-3 for the application as a medicament. Pharmaceutical composition to be applied as a medicament comprising the compound or compounds according to any of claims 1-3 as the active agent and a pharmaceutically acceptable support. Use of the compound or compounds according to any of claims 1-3 for the manufacture of a medicament for the application as an antibiotic or anti-tumor agent.

Inventors: ASANO, KOZO, ASHIZAWA, TADASHI, NAKANO, HIROFUMI, TAKAHASHI, ISAMI, TAKAHASHI, KEIICHI, TOMITA, FUSAO, YASUZAWA, TORU
Applicants: KYOWA HAKKO KOGYO CO., LTD.
Application year: 1987
Main classification: C07H7
IPC codes: C12P19, C07H17, C07H15