Preparation of a new diosmine derivate

Abstract: The invention refers to the preparation of aluminium and diosmin octasulfate, a new derivative of diosmin, by reaction of diosmin with a sulfating agent, such as sulfur trioxide-pyridine complex, sulfur trioxide-trimethylamine complex and sulfur trioxide-triethylamine complex, at a temperature between 40º and 110º C, in an appropiate medium, with preference pyridine, dimethylformamide and dimethylacetamide, and final addition of aluminium hydroxychloride.

Description: Diosmine is a polyhydroxylated flavone with eight hydroxyl groupe, two of them are phenolic and the other six being in the sugar rest, and is able to undergo different reactions being esterification one of them. Diosmine can give esters when it is treated with a reactive derivative of organic and inorganic acids in a suitable solvent. In particular Diosmine forms esters from sulfuric acid when it reacts with a sulfating agent, varying the esterification index with the conditions of the reaction, especially with the molar ratio of diosmine/sulfating agent. In certain conditions it is possible to esterify all the hydroxyl groups of the molecule and to afford the octasulfate of diosmine. The sulfur trioxide-pyridine complex or the sulfur trioxide-trialkylamine complexes are used as sulfating agents. The first of these is obtained from chlorosulfonic acid or from the sulfur trioxide and the pyridine, and it can be prepared and purified previous to its use or formed in situ in the reaction vessel. The sulfur trioxide-pyridine complex sulfates substances such as hydrazine, diethylamine, phenol and naphthalene, and it has been used in preparation of carbohydrate, sterol and phenol sulfate esters. The sulfur trioxide-trialkylamine complexes, especially those formed with trimethylamine and triethylamine, are also useful to carry out the reaction. They are mild agents, sulfating alcohols or phenols. In the present invention, the preparation of a new diosmine derivative is described. The diosmine derivative is an diosmin octakis (hydrogensulfate) aluminium complex which has a great pharmacological interest with molecular formula: in which R represents SO₃Al₂(OH)₅ The product administered orally in doses of 200 mg/kg. rats with gastric lesions induced by pure ethanol (following the technique of A. Robert et al., Gastroenterology, 77 , 433-443 (1979) significantly protected the gastric mucosa. In another experiment carried out in accordance with Shay et al's method (Gastroenterology, 5, 43-61 (1945)) the diosmin octakis (hydrogen sulfate) aluminium complex significantly protected the glandular and aglandular region of the stomach at doses of 100 and 200 mg/kg., when it was administered orally inmediately after the pilorus ligation. Besides the gastric mucosa protective activity and acid-consuming capacity of the diosmin octakis (hidrogen sulfate) aluminium complex, it lacks of undesirable side effects. The preparation of the diosmin octakis (hidrogen sulfate) aluminium complex is carried out by the reaction of the diosmine with the sulfur trioxide-pyridine complex or with the sulfur trioxide-trialkylamine complexes, using the sulfating agents in an excess. The solvent exployed in the sulfation of Diosmine is chosen among pyridine, dimethylformamide and dimethylacetamide and the temperatura at which the reaction takes places can oscilate between 40º and 110º C without a substantial variation in the result of the process, but logically at low temperatures the completion of the reaction takes more time. The aluminium salt of the diosmine octasulfate can be made from the corresponding sodium salt or from the corresponding ammonium salt by adding aluminium hydroxychloride in an aqueous solution. The chemical structure of this compound is represented in (I) where R = -SO₃Al₂(OH)₅. The following examples of the preparation of the diosmin octakis (hydrogen sulfate) aluminium complex ilustrate the invention but are not exhaustive. EXAMPLE 1 A mixture of 10 g. of diosmine and 25 g, of the sulfur trioxide-pyridine complex in 100 ml. of pyridine is heated at a temperature of 60º C for 5 hours with stirring. The upper layer of pyridine is separated by decantation and the lower one is neutralized with concetrated aqueous sodium hydroxide. Ethyl alcohol is added dropwise until a precipitate begins to form. This deposit is filtered off and more ethyl alcohol is added to the liquid part. The new solid formed is separated by filtration and is washed times with ethyl alcohol. It is dried at room temperature in vacum (16,7 g.). It is dissolved in 200 ml. of distilled water and 130 ml. of a 15% solution of aluminium hydroxychloride is added. The resulting yellow solid is filtered, washed several times with water and is dried in vacuum at room temperature (26 g). M.P. Higher than 250º C. IR (3490, 1640 cm⁻¹). C₂₈H₆₄Al₁₆O₇₉S₈, calculated Al 18,34 S 10.90 found 18,71 10,81 EXAMPLE 2 10 g. of diosmine and 24 g. of the sulfur trioxide-trimethylamine complex is heated in 40 ml. of dimethylformamide for 2 hours at a temperature of 90 ºC. The mixture is cooled at room temperature and then poured into 400 ml. of acetone with stirring. the upper layer is separated by decantation and the lower layer is washed twice with acetone. It is then dissolved in water and 150 ml. of a 15% solution of aluminium hydroxychloride is added. The solid which forms is filtered, washed in water and dried. 17 g. of the product described in Example 1, are obtained.

Claims: Diosmin octakis (Hydrogen sulfate) aluminium complex, with molecular formula: in which R represents SO₃ Al₂ (OH)₅ A process for the preparation of the compound as claimed in claim 1 which comprises reacting diosmine with a sulfating agent in proper conditions resulting in the diosmine octasulfate which is futher isolated as an aluminium salt. A process as claimed in claim 2 in which the sulfating agent is selected from the sulfur trioxide-pyridine complex, the sulfur trioxide-trimethylamine complex and the sulfur trioxide-triethylamine complex. A process as claimed in claim 2 in which the solvent of the sulfating reaction is selected from pyridine, dimethylformamide and dimethylacetamide. A process as claimed in claim 2 in which the reaction of diosmine with the sulfating agent takes place at temperatures between 40º and 110 ºC.

Inventors: PESTANA RAMON MOSQUERA, VENERO AURELIO ORJALES, PESTANA, RAMON MOSQUERA, VENERO, AURELIO ORJALES
Applicants: ESPANOLA PROD QUIMICOS, FABRICA ESPANOLA DE PRODUCTOS  QUIMICOS Y FARMACEUTICOS, S.A.
Application year: 1987
Main classification: C07H17
IPC codes: A61K31, A61P1, C07H17