MAMMALIAN INTERLEUKIN-4

Abstract: Mammalian proteins and muteins thereof, designated interleukin-4s (IL-4s), are provided which exhibit both B cell growth factor activity and T cell growth factor activity. Compounds of the invention include native human and murine IL-4s, muteins thereof, and nucleic acids which are effectively homologous to disclosed cDNAs, and/or which are capable of coding for mammalian IL-4s and their muteins.

Description: MAMMALIAN I TERLEUKIN-4 The present invention relates generally to protein and utein factors of the mammalian immune system and to nucleic acids coding therefor. More particularly, the invention relates to protein and mutein factors (along with their encoding nucleic acids) which exhibit both T cell growth factor activity and B cell growth factor activity. Recombinant DNA technology refers generally to the technique of integrating genetic information from a donor source into vectors for subsequent processing, such as through introduction into a host, whereby the transferred genetic information is copied and/or expressed in the new environment. Commonly, the genetic information exists in the form of complementary DNA (cDNA) derived from messenger RNA (mRNA) coding for a desired protein product. The carrier is frequently a plasmid having the capacity to incorporate cDNA for later replication in a host and, in some cases, actually to control expression of the cDNA and thereby direct synthesis of the encoded product in the host. For some time, it has been known that the mammalian immune response is based on a series of complex cellular interactions, called the "immune network." Recent research has provided new insights into the inner workings of this network. While it remains clear that uch of the response does, in fact, revolve around the network-like interactions of lymphocytes, macrophages, granulocytes and other cells, immunologists now generally hold the opinion that soluble proteins (e.g., the so- called "lymphokines" or "monokines") play a critical role in controlling these cellular interactions. Thus, there is considerable interest in the isolation, characterization, and mechanisms of action of cell modulatory factors, an understanding of which should yield significant breakthroughs in the diagnosis and therapy of numerous disease states. Lymphokines apparently mediate cellular activities in a variety of ways. They have been shown to support the proliferation, growth and differentiation of the pluripotential hematopoietic stem cells into the vast number of progenitors composing the diverse cellular lineages responsible for the immune response. These lineages often respond in a different manner when lymphokines are used in conjunction with other agents. Cell lineages that are especially important to the immune response include two classes of lymphocytes: B-cells, which can produce and secrete immunoglobulins (proteins with the capability of recognizing and binding to foreign matter to effect its removal) , and T-cells of various subsets. hat secrete lymphokines and induce or suppress the B-cells and some of the other cells (including other T-cells) making up the immune network. Another important cell lineage is the mast cell (which has not been positively identified in all mammalian species)—a granule-containing connective tissue cell located proximal to capillaries throughout the body, with especially high concentrations in the lungs, skin, and gastrointestinal and genitourinary tracts. Mast cells play a central role in allergy- related disorders, particularly anaphylaxis, as follows: when selected antigens crosslink one class of i munoglobulins bound to receptors on the mast cell surface, the mast cell degranulates and releases the mediators (e.g., histamine, serotonin, heparin, prostaglandins, etc.) which cause allergic reactions, e.g., anaphylaxis. Research to better understand (and thus potentially treat therapeutiσally) various immune disorders has been hampered by the general inability to maintain cells of the immune system in vitro. Immunologists have discovered that culturing these cells can be accomplished through the use of T-cell and other cell supernatants, which contain various growth factors, such as some of the lymphokines. The detection, isolation and purification of factors such as lymphokines and other soluble mediators of immune reactions is extremely difficult, and is frequently complicated by the very nature of the supernatants they are typically located in, the divergencies and cross-overs of activities of the various components in the mixtures, the sensitivity (or lack thereof) of the assays utilized to ascertain the factors' properties, the frequent similarity in the range of molecular weights and other characteristics of the factors, and the very low concentration of the factors in their natural setting. As more lymphokines become available, primarily through molecular cloning, interest has heightened in finding clinical applications for them. Because of physiological similarities to hormones (e.g., soluble factors, growth mediators, action via cell receptors), potential uses of lymphokines have been analogized to the current uses of hormones, e.g. Dexter, Nature, Vol. 321, pg. 198 (1986). One hope is that the levels of lymphokines in a patient can be manipulated directly or indirectly to bring about a beneficial immune response, e.g. suppression of inflammation, allergy, or tissue rejection, or stimulation or potentiation against infection or malignant growth. Other potential clinical uses of lymphokines include maintaining and expanding in vitro populations of certain immune system cells of one person for eventual reintroduction into the same or another person for a beneficial effect. For example, investigations are currently underway to determine whether populations of lymphokine-activated killer T cells of a patient can be expanded outside his or her body and then reinjected to bring about an enhanced antitumor response. Another potential clinical use of lymphokines, particularly colony stimulating factors, such as granulocyte-macrophage colony stimulating factor (GM-CSF) , and factors which enhance their activities, is stimulating blood cell generation, for example in pre- or post-chemotherapy or radiation therapy against tumors, in treatment of myeloid hypoplasias, or in treatment of neu-trophil deficiency syndromes: Dexter, Nature, Vol. 321, pg. 198 (1986). Such factors would also be useful in bone marrow transplant therapy, which is being used increasingly to treat aplastic anemia and certain leukemias. There are two properties of lymphokines that have important consequences for such clinical applications: individual lymphokines are frequently pleiotropic; and the biological effects of one lymphokine can usually be modulated by at least one other lymphokine, either by inhibition or by potentiation. For example, tumor necrosis factor, which synergizes with gamma-interferon, stimulates interleukin-1 (IL-1) production and can activate the phagocytic activity of neutrophils. IL-1, a protein produced by activated macrophages, mediates a wide range of biological activities, including stimulation of thymocyte proliferation via induction of interleukin-2 (IL-2) release, stimulation of 3-lymphocyte maturation and - 3- prolif ration, fibroblast growth factor activity and induction of acute-phase protein synthesis by hepatocytes. IL-1 has also been reported to stimulate prostaglandin and collagenase release from synovial cells, and to be identical to endogenous pyrogen: Krampschmidt, J. Leuk. Biol. , Vol. 36, pgs. 341-355 (1984) . Interleukin-2, formerly referred to as T-cell growth factor, is a lymphokine which is produced by lectin- or antigen-activated T cells. The reported biological activities of IL-2 include stimulation of the long-term in vitro growth of activated T-cell clones, enhancement of thymocyte mitogenesis, and induction of cytotoxic T-cell reactivity and plaque-forming cell responses in cultures of nude mouse-spleen cells. In addition, like interferons (IFNs) , IL-2 has been shown to augment natural killer cell activity, suggesting a potential use in the treatment of neoplastic diseases: Henney et al. , Nature, Vol, 291, pgs. 335-338 (1981). Some success has been reported in such therapy, e.g. Lotze and Rosenberg, "Treatment of Tumor Patients with Purified Human Interleukin-2," pgs. 711-719, in Sorg et al. , Eds. Cellular and Molecular Biology of Lymphokines (Academic Press, Inc., New York, 1985); and Rosenberg and Lotze, "Cancer Immunotherapy Using Interleukin-2 and Interleukin-2-Activated Lymphocytes," Ann. Rev. Immunol. , Vol. 4, pgs. 681-709 (1986). However, IL-2 toxicity has limited the dosages which can be delivered to cancer patients for taking advantage of these properties: Lotze and Rosenberg, pgs. 711-719; and Welte et al., pgs. 755- 759, in Sorg et al. Eds. (cited above) . Metcalf, D., The Hematopoietic Colony Stimulating Factors, (Elsevier, Amsterdam, 1984), provides an overview of research concerning lymphokines and various growth factors involved in the mammalian immune response. Yung, Y.-P., et al. , J. Immunol. Vol. 127 pg. 794 (1981), describe the partial purification of the protein of approximately 35 kd exhibiting mast cell growth factor (MCGF) activity and its separation from interleukin-2 (IL-2) , also known as T-cell growth factor (TCGF) . Nabel, G. , et al. , Nature, Vol. 291, pg. 332 (1981) report an MCGF exhibiting a molecular weight of about 45 kd and a pl of about 6.0. Clark-Lewis, I. and Schrader, J., J. Immunol., Vol. 127, pg. 1941 (1981), describe a protein having mast cell like growth factor activity that exhibits a molecular weight of about 29 kd in phosphate-buffered saline and about 23 kd in 6M guanidine hydrochloride, with a pi of about 4-8 but of about 6-8 after neuraminidase treatment. Murine IL-2 and interleukin-3 (IL-3) have been partially characterized biochemically by Gillis, S., et al. , J. Immunol. , Vol. 124, pgs. 1954-1962 (1980), and Ihle, J., et al. , J. Immunol. , Vol. 129, pgs. 2431-2436 (1982), respectively, with IL-2 having an apparent molecular weight (probably as a dimer) of about 30-35 kd and IL-3 having a molecular weight of about 28 kd. Human IL-2 apparently has a molecular weight of about 15 kd and is described by Gillis, S., et al. , Immu. Rev. , Vol. 63, pgs. 167-209 (1982) . Comparison between IL-3 and MCGF activities of T-cell supernatants have been reported by Yung Y. and Moore, M. , Contemp. Top. Mol. Immunol., Vol. 10, pgs. 147-179 (1985), and Rennick, D., et al., J. Immunol. , Vol. 134, pgs. 910-919 (1985). An extensive literature exists concerning the regulation of B-cell growth and differentiation by soluble factors: e.g. for reviews see Howard and Paul, Ann. Rev. Immunol. , Vol. 1, pgs. 307-333 (1983); Howard et al., Immunol. Rev. , 1984, No. 78, pgs. 185-210; Kishimoto et al., Immunol. Rev., 1984, No. 78 pgs. 97- 118; and Kishimoto, Ann. Rev. Immunol. , Vol. 3, pgs. 133- 157 (1985) . Some confusion has existed over the nomenclature used for labeling the various factors because of differences in source materials, difficulties in purification, and differences in the assays used to define their biological activities. Consensus in regard to nomenclature apparently has been reached in some cases: Paul, Immunology Today, Vol. 4, pg. 322 (1983); and Paul, Molecular Immunol. , Vol. 21, pg. 343 (1984) . B-cell growth factor (BCGF) activity is characterized by a capacity to cause DNA synthesis in B cells co- stimulated by exposure to anti-IgM, or like antigens. It is believed that interleukin-1 (IL-1) is also required for BCGF activity to be manifested, at least when the assay is conducted with low densities of B cells. Alternative assays for human BCGF have been described: e.g. Maizel et al, Proc. Natl. Acad. Sci., Vol. 80, pgs. 5047-5051 (1983) (support of long-term growth of human B cells in culture) . The activity associated with the former assay has also been labelled B cell stimulatory factor-1 (BSF-1) activity and BCGF I, to distinguish it from similar and/or related activities. In particular, an activity designated BCGF II has been described. It is characterized by a capacity to cause DNA synthesis in mitogen-stimulated B cells or in transformed B cell lines. Mitogens associated with BCGF II activity include dextran sulfate, lipopolysaccharide, and Staphylococcus extracts. BCGF I registers no response in these assays. In humans it is believed that BCGF II is a molecule having a molecular weight of about 50 kilodaltons (kD) , and that it acts synergistically with BCGF I (i.e. BSF-1) in promoting B cell proliferation in an immune response: Yoshizaka et al., J. Immunol. , Vol. 130, pgs. 1241-1246 (1983). Howard et al., J. Ξxp. Med. , Vol. 155, pgs. 914-923 (1982) were the first to show the existence of a murine BCGF (later to be called variously BCGF I, BSF-1, or l gG ] _ induction factor) distinct from inte leukin-2. Similar observations were reported almost simultaneously for a human system by Yoshizaki et al. , J_;_ Immunol. , Vol. 128, pgs. 1296-1301 (1982), and later by Okada et al., J. Exp. Med., Vol. 157, pgs. 583-590 (1983) . Biochemical and biological characterization of molecules exhibiting BCGF, or BSF-1, activity has progressed steadily since these initial discoveries. Maizel et al. , Proc. Natl. Acad. Sci., Vol. 79, pgs. 5998-6002 (1982) , have reported a trypsin-sensitive human BCGF having a molecular weight of 12-13 kD and an isoelectric point (pi) of about 6.3-6.6. Farrar et al., J. Immunol., vol. 131, pgs. 1838-1842 (1983) reported partial purification of a heterogeneous murine BCGF having molecular weights of 11 and 15 kD by SDS-PAGE and pis of 6.4-8.7. Ohara and Paul, in Nature, Vol. 315, pgs. 333-336 (1985) describe a monoclonal antibody specific for murine BSF-1, and give molecular weights for BSF-1 of 14 kD and 19-20 kD with pi of 6.7. Butler et al., J. Immunol., Vol. 133, pgs. 251-255 (1984), report a human BCGF having a molecular weight of 18-20 kD and a pi of 6.3-6.6. Rubin et al. , Proc. Natl. Acad. Sci., vol. 82, pgs. 2935-2939 (1985), report that pre-incubation of resting B cells with BSF-1 prior to exposure to anti-IgM antibodies increases cell volume, and later speeds entry to S phase upon exposure to anti-IgM antibodies. Vitetta et al, J. Exp. Med., Vol. 162, pgs. 1726-1731 (1985), describe partial purification of murine 3SF-1 by reverse phase HPLC of serum-free supernatants of EL-4 cells. SDS-PAGE indicated a protein of about 20-22kD. Ohara et al. , J. Immunol. , Vol. 135, pgs. 2518-2523 (1985) also report partial purification of murine BSF-1 by a similar procedure, and report the factor to be a protein of about 18-21.7 kD. Sideras et al. , in Eur. J. Immunol. , Vol. 15, pgs. 586-593, and 593-598 (19 ' 85) , report partial purification of a murine IgG ] _-inducing factor, that is a 3SF-1, and report the factor to be a protein of about 20 kD having pis of 7.2-7.4 and 6.2-6.4; and Smith and Rennick, in Proc. Natl. Acad. Sci., Vol. 83, pgs. 1857- 1861 (1986) , report the separation of a factor from IL-2 and IL-3 which exhibits T cell growth factor activity and mast cell growth factor activity. Later, Noma et al. , Nature, Vol. 319, pgs. 640-646 (1986), cloned and sequenced a nucleic acid coding for the Sideras et al. factor, and Lee et al. , Proc. Natl. Acad. Sci., Vol. 83, pgs. 2061-2065 (1986) cloned and sequenced a nucleic acid coding for the Smith and Rennick factor. More recently, Grabstein et al., J. Exp. Med., vol. 163, pgs. 1405-1414 (1986) , report purifying and sequencing murine BSF-1. Milanese, et al, in Science, Vol. 231, pgs. 1118-1122 (1986) , report a lymphokine unrelated to 3SF-1 which they provisionally designate IL-4A. Their IL-4A is a 10-12 kD protein secreted from helper T cells after cross-linking of T3-Ti receptors. It stimulates resting lymphocytes via interaction with Til receptors and subsequent induction of interleukin-2 (IL-2) receptors. Sanderson et al. , in Proc. Natl. Acad. Sci., Vol. 83, pgs. 437-440 (1986), proposed that the name interleukin 4 be given to eosinophil differentiation factor based on evidence that it is apparently the same as 3 cell growth factor II. From the foregoing it is evident that the discovery and development of new lymphokines could contribute to the development of therapies for a wide range of degenerative conditions which directly or indirectly involve the immune system and/or hematopoietic cells. In particular, the discovery and development of lymphokines which enhance or potentiate the beneficial activities of known lymphokines would be highly advantageous. For example, the dose-limiting toxicity of IL-2 in tumor therapy could be reduced by the availability of a lymphokine or cofactor with potentiating effects; or the efficacy of bone marrow transplants could be increased by the availability of factors which potentiate the activities of the colony stimulating factors. The present invention is directed to mammalian interleukin-4 (IL-4). It includes nucleic acids coding for polypeptides exhibiting IL-4 activity, as well as the polypeptides themselves and methods for their production. The nucleic acids of the invention are defined (1) by their homology to cloned complementary DNA (cDNA) sequences disclosed herein, and (2) by functional assays for IL-4 activity applied to the polypeptides encoded by the nucleic acids. As used herein, the term "IL-4 activity" in reference to a protein or a polypeptide means that the protein or polypeptide exhibits both B-cell growth factor (BCGF) activity and T cell growth factor (TCGF) activity. For a given mammal, IL-4 activity is determined by species-specific TCGF and BCGF assays. AS explained more fully below, specific embodiments of IL-4 can be further characterized by additional assays. For example, some forms of murine IL- 4 exhibit mast cell growth factor (MCGF) activity; some forms of both human and murine IL-4 potentiate the TCGF activity of IL-2; some forms of both murine and human IL- 4 potentiate GM-CSF stimulated proliferation in certain cell types; some forms of both human and murine IL-4 can induce Fσ-epsilon receptor expression on B cells; and some forms of both human and murine IL-4 can induce the expression of major histocompatibility complex (MHC) antigens on B cells: the class II DR antigen on human 3 cells, and the la antigen on mouse B cells. The invention is based in part on the discovery and cloning of cDNAs which are capable of expressing proteins having IL-4 activity. cDNA clones of the invention include human cDNA inserts of plasmid vectors "clone 46" (also referred to herein as pcD-2Fl-l3 or pcD- 46) and "clone 125" (also referred to herein as ρcD-125) ; and mouse cDNA insert of plasmid vector ΌCD-2A-E3. The three vectors are deposited with the American Type Culture Collection (ATCC), Rockville, MD, under ATCC accession numbers 53337, 67029, and 53330, respectively. The invention includes nucleic acids having nucleotide sequences which are effectively homologous to the cDNA clones of the invention and which express IL-4 activity. Nucleic acids and proteins of the invention can be derived from the above-mentioned cDNAs by standard techniques for mutating nucleic acid sequences. They can be prepared de novo from immune system-derived cell lines, such as T cell hybridomas, which contain or can be induced to contain messenger RNA (mRNA) sequences coding for IL-4; and they can be obtained by probing DNA or RNA extracts or libraries with probes derived from the cDNA clones of the invention. The term "effectively homologous" as used herein means that the nucleotide sequence is capable of being detected by a hybridization probe derived from a cDNA clone of the invention. The exact numerical measure of homology necessary to detect nucleic acids coding for IL-4 activity depends on several factors including (1) the homology of the probe to non-IL-4 coding sequences associated with the target nucleic acids, (2) the stringency of the hybridization conditions, (3) whether single stranded or double stranded probes are employed, (4) whether RNA or DNA probes are employed, (5) the measures taken to reduce nonspecific binding of the probe, (6) the nature of the label used to detect the probe, (7) the fraction of guanidine and cytosine bases in the probe, (8) the distribution of mismatches between probe and target, (9) the size of the probe, and the like. Preferably, an effectively homologous probe derived from the cDNA of the indention is at least fifty percent (50%) homologous to the sequence to be isolated. More preferably, the effectively homologous probe is at least seventy-five to eiqhty percent (75-80%) homologous to the sequence to be isolated. Most preferably, the effectively homoloqous probe is at least ninety percent (90%) homoloqous to the sequence to be isolated. Homology as the term is used herein is a measure of similarity between two nucleotide (or amino acid) sequences. Homology is expressed as the fraction or percentage of matching bases (or amino acids) after two sequences (possibly of unequal length) have been aligned. The term alignment is used in the sense defined by Sankoff and Kruskal in chapter one of Time Warps, String Edits, and Macromolecules: The Theory and Practice of Sequence Comparison (Addison-Wesley, Reading, MA, 1983). Roughly, two sequences are aligned by maximizing the number of matching bases (or amino acids) between the two sequences with the insertion of a minimal number of "blank" or "null" bases into either sequence to bring about the maximum overlap. Given two sequences, algorithms are available for computing their homology: e.g. Needleham and Wunsch, J. Mol. Biol. , Vol. 48, pqs. 443-453 (1970); and Sankoff and Kruskal (cited above) pqs. 23-29. Also, commercial services are available for performing such comparisons, e.g. Intelligenetics, Inc. (Palo Alto, CA) . The present invention also relates to the natural mammalian growth factors (polypeptides) exhibiting a broad activity spectrum for a variety of cells inteqral to the immune response. Methods of producing such factors and their use for in vivo treatment of mammals are provided. Such polypeptide compositions include a mammalian factor in substantially pure form capable of exhibitinq B-cell, T-cell and mast cell stimulatory activity. These factors, which were originally isolated from the supernatant fluid of various T cells, include natural polypeptides exhibiting, under non-reducing conditions, a molecular weight of about 20 kd or 15 kd, or active fragments of such polypeptides. The factors may be used alone or in conjunction with other compounds for various diagnostic and therapeutic purposes, including the production of specific antibodies. A preferred embodiment of the invention is the set of glycosylated or unglycosylated human IL-4 proteins and muteins defined by the following formula: X(His) - X(Lys) - X(Cys) - X(ASp) - X(Ile) - X(Thr) X(Leu) - X(Gln) - X(Glu) - X(Ile) - X(Ile) - X(Lys) X(Thr) - X(Leu) - X (Asn) - X(Ser) - X(Leu) - X(Thr) X(Glu) - X(Gln) - X(Lys) - X(Thr) - X(Leu) - X(Cys) X(Thr) - X(Glu) - X(Leu) - X(Thr) - X(Val) - X(Thr) X(Asp) - X(Ile) - X(Phe) - X(Ala) - X(Ala) - X(Ser) X(Lys) - X(Asn) - X(Thr) - X(Thr) - X(Glu) - X(Lys) X(Glu) - X(Thr) - X(Phe) - X(Cys) - X(Arg) - X(Ala) X(Ala) - X(Thr) - X(Val) - X(Leu) - X(Arg) - X(Gln) X(Phe) - X(Tyr) - X(Ser) - X(His) - X(His) - X(G ' lu) X(Lys) - £(ASp) - X(Thr) - X(Arg) - X(Cys) - X(Leu) X(Gly) - X(Ala) - X(Thr) - X(Ala) - X(Gln) - X(Gln) X(Phe) - X(His) - X(Arg) - X(His) - X(Lys) - X(Gln) X(Leu) - X(Ile) - X(Arg) - X(Phe) - X(Leu) - X(Lys) X(Arg) - X(Leu) - X(Asp) - X(Arg) - X(Asn) - X(Leu) X(Trp) - X(Gly) - X(Leu) - X(Ala) - X(Gly) - X(Leu) X(Asn) - X(Ser) - X(Cys) - X(Pro) - X(Val) - X(Lys) X(Glu) - X(Ala) - X (Asn) - X(Gln) - X(Ser) - X(Thr) X(Leu) - X(Glu) - X (Asn) - X(Phe) - X(Leu) - X(Glu) X(Arg) - X(Leu) - X(Lys) - X(Thr) - X(Ile) - X(Met) X(Arg) - X(Glu) - X(Lys) - X(Tyr) - X(Ser) - X(Lys) X(Cys) — X(Ser) - X(Ser) Formula I wherein the term X(Xaa) represents the group of synonymous amino acid ' s to the amino acid Xaa. Synonymous amino acids within a group have sufficiently similar physicochemical properties for substitution between members of the group to preserve the biological function of the molecule: Grantham, Science, vol. 185, pgs. 862- 864 (1974). It is clear that insertions and deletions of amino acids may also be made in the above-defined sequence without altering biological function, particularly if the insertions or deletions only involve a few amino acids, e.g. under ten, and do not remove or displace amino acids which are critical to a functional conformation, e.g. cysteine residues, Anfinsen, "Principles That Govern The Folding of Protein Chains", Science, Vol. 181, pgs. 223-230 (1973). Proteins and muteins . produced by such deletions and/or insertions come within the purview of the present invention. Whenever amino acid residues of the protein of Formula I are referred to herein by number, such number or numbers are in reference to the N-terminus of the protein. Preferably the synonymous amino acid qroups are those defined in Table I. More preferably, the synonymous amino acid groups are those listed before the second slash in each line in Table I; and most preferably the synonymous amino acid groups are those listed before the first slash in each line in Table II. Table I. Preferred Groups of Synonymous Amino Acids Amino Acid Synonymous Group Ser Ser,//Thr, Gly, Asn Arg Arg,/His, Lys,/Glu, Gin Leu Leu, He, Met,/Phe,/Val , Tyr Pro Pro,/Ala,/Thr, Gly Table I (continued) Amino Acid Synonymous Group Thr Thr,//Pro, Ser, Ala, Gly, His, Gin Ala Ala,/Pro,/Gly, Thr Val Val,/Met, Ile/Tyr, Phe, Leu, Val Gly Gly,//Ala, Thr, Pro, Ser He He, Met, Leu,/Phe, Val,/He, Tyr Pmhee Phe,/Met, Tyr, He, Leu,/Trp, Val Tyr Tyr,/Phe,/Trp, Met, He, Val, Leu, Cys Cys, Ser,//Thr His His,/Gln, Arg,/Lys, Glu, Thr Gin Gln,/Glu, His,/Lys, Asn, Thr, Arg Asn Asn,/Asp,/Se , Gin Lys Lys,/Arg,/Glu, Gin, His Asp Asp,/Asn,/Glu Glu Glu,/Gln,/ Asp, Lys, Asn, His, Arg Met Met, He, Leu,/Phe, Val/ The invention includes the polypeptides of Formula I with amino acid substitutions (between an amino acid of the native human IL-4 and a synonymous amino acid) at a single position or at multiple positions. The term "N-fold substituted" is used to describe a subset of polypeptides defined by Formula I wherein the native amino acids have been substituted by synonymous amino acids at at least N positions. Thus, for example, the group of 1-fold substituted polypeptides of Formula I consists of 559 polypeptides for the preferred groups of synonymous amino acids, 189 for the more preferred groups of synonymous amino acids, and 56 for the most preferred groups of synonymous amino acids. These numbers were arrived at by summing the number of amino acids of each kind in the native chain times the size of the synonymous amino acid group for that amino acid. Preferably the group of human IL-4 polypeptides consists of the 10-fold substituted polypeptides of Formula I; more preferably it consists of 3-fold substituted polypeptides of Formula I; and most preferably it consists of 1-fold substituted polypeptides of Formula I, which in particular includes the native human IL-4 polypeptide whose sequence is illustrated in Figure IC of the drawings. Likewise, the term "N-fold inserted" in reference to the peptides of Formula I is used to describe a set of polypeptides wherein from 1 to N amino acids have been inserted into the sequence defined by Formula I. Preferably, the inserted amino acids are selected from the preferred groups of synonymous amino acids (Table I) of the amino acids flanking the insertion; more preferably they are selected from the more preferred groups of synonymous amino acids (Table II) of the amino acids flanking the insertion, and most preferably they are selected from the most preferred groups of synonymous amino acids (Table III) of the amino acids flanking the insertion. Thus, for example, one subgroup of the group of 1-fold inserted peptides comprises an amino acid inserted between the N-terminal X(His) and the adjacent X(Gly). The insertions defining the members of this subgroup are preferably selected from the group consisting of Pro, Ala, Gly, Thr, Ser, Gin, Glu, Arg, His, and Lys; more preferably they are selected from the group consisting of Gly, His, Gin and Arg, and most preferably they are selected from the group consisting of His and Gly. Insertions can be made between any adjacent amino acids of Formula I. Since there are 128 possible insertion locations, and since multiple insertions can be made at the same location, a 2-fold inserted peptide of Formula I gives rise to 16,384 subgroups of peptides, and the size of each subgroup depends on the sizes of the synonymous amino acid groups of the amino acids flanking the insertions. The term "N-fold deleted" in reference to the polypeptides of Formula I is used to describe a set of peptides having from 1 to N amino acids deleted from the sequence defined by Formula I. Thus, the set of 1-fold deleted polypeptides of Formula I consists of 129 subgroups of polypeptides each 128 amino acids in length (128-mers). Each of the subgroups in turn consists of all the 128-mers defined by the preferred, more preferred, and most preferred synonymous amino acid qroups. The above preferred embodiment of the invention further includes nucleotide sequences effectively homologous to or capable of encodinq the polypeptides of Formula I for the preferred, more preferred, and most preferred groups of synonymous amino acids. More preferably said nucleotide sequences are capable of encoding the polypeptides of Formula I for the preferred, more preferred, and most preferred qroups of synonymous amino acids. In particular, the invention includes native human IL-4, the amino acid sequence of which is illustrated in Figure IC, and all nucleotide sequences capable of encodinq it. Throughout, standard abbreviations are used to designate amino acids, nucleotides, restriction endonucleases, and the like: e.g. Conn, "Nomenclature and Symbolism of .-Amino Acids, "Methods in Enzymoloqy, Vol. 106, pgs. 3-17 (1984); Wood et al . Biochemistry: A Problems Approach, 2nd ed. (Benjamin, Menlo Park, 1981); and Roberts, "Directory of Restriction Endonucleases", Methods in Enzymoloqy, Vol. 68, pqs. 27-40 (1979). The present invention is addressed to problems associated with the application of immunorequla o y agents to treat medical and/or veterinary disorders. In particular, it provides compounds which have beneficial effects when used alone or which can act in concert with other lymphokines and immune-system mediators to produce beneficial effects. Preferred embodiments of the invention will now be described, partly in conjunction with the accompanying drawings (Figs. 1 to 19) . Figure lA illustrates the nucleotide sequence and deduced amino acid sequence of the insert of vector ρcD-2A-E3, which expresses murine IL-4. Figure IB illustrates the nucleotide sequence and deduced amino acid sequence of the insert of vector pcD-125, which expresses human IL-4. Figure IC illustrates the amino acid sequence of purified native human IL-4 expressed and secreted by COS 7 monkey cells transfeσted with pcD-125. Figure 2A is a map of vector pcD-2A-E3, the insert of which codes murine IL-4. Figure 2B is a restriction endonuclease cleavage map of the insert of vector pcD-2A-E3. Figure 2C is a map of vector pcD-46, the insert of which codes human IL-4. Figure 2D is a restriction endonuclease cleavage map of the insert of vector pcD-46. Figure 3A illustrates relative TCGF activities (over a range of dilutions) of various culture supernatants including one (curve 1) from pcD-2A-E3- transfected COS 7 cells. Figure 3B illustrates relative MCGF activities (over a range of dilutions) of various culture supernatants including one (curve 1) from pcD-2A-E3- transfected COS 7 cells. Figure 3C illustrates the relative degrees of la induction produced by the indicated amounts of supernatant from pcD-2A-E3-transfected COS 7 cells (curve 1 ) , Cl.Lvl + 2"/9 cells (curve 2), and mock transfeσted COS 7 cells (curve 3) . Figure 3D graphically illustrates the extent of IgE and IgG- ] _ induction by supernatants from pcD-2A-E3- transfected COS 7 cells and various controls in T cell- depleted mouse spleen cells. Figure 4A illustrates the TCGF activities of several pcD-125 transfection supernatants and controls as measured by a colorimetric proliferation assay on the factor-dependent human helper T cell line, JL-EBV. Figure 4B illustrates the TCGF activities of a pcD-125 transfection supernatant and controls as measured by a colorimetric proliferation assay on PHA-stimulated peripheral blood lymphocytes. Figure 4C illustrates the TCGF activities of a pcD-125 transfection supernatant and controls as measured by tritiated thymidine incorporation by PHA-stimulated peripheral blood lymphocytes. Figure 5A is a histogram of cell frequency versus fluorescence intensity for a control population of stimulated human tonsilar B cells whose Fc-epsilon receptors have been fluorescently labeled. Figure 5B is a histogram of cell frequency versus fluorescence intensity for a population of stimulated human tonsilar B cells which had been exposed to medium consisting of 0.1% supernatant from pcD-125- transfected COS 7 cells and whose Fc-epsilon receptors have been fluorescently labeled. Figure 5C is a histogram of cell frequency versus fluorescence intensity for a population of stimulated human tonsilar B cells which had been exposed to medium consisting of 1% supernatant from pcD-125- transfected COS 7 cells and whose Fc-epsilon receptors have been fluorescently labeled. Figure 5D is a histogram of cell frequency versus fluorescence intensity for a population of stimulated human tonsilar B cells which had been exposed to medium consisting of 10% supernatant from ρcD-125- transfected COS 7 cells and whose Fc-epsilon receptors have been fluorescently labeled. Figure 6A illustrates the nucleotide sequence of a synthetic human IL-4 gene useful for expressing native or mutant IL-4s in E. coli. Figure 6B is a restriction endonuclease cleavage map of a synthetic human IL-4 gene inserted in plasmid pϋ * Cl8. Figures 7A-7F respectively illustrate the double stranded DNA fragments 1A/B through 6A/B used to construct a synthetic human IL-4 gene. Figure 8 illustrates nucleotide sequences adjacent to the initiator ATG codon in the E. coli expression vector TAC-RBS. The sequences commence at an EcoRI restriction site and end with a Hindlll site. The ribosome binding sequence (RBS) showing complementarity to the 3' end of 16S ribosomal RNA is underlined, and the ATG initiator codon is underlined. Figure 9 illustrates histograms of cell frequency versus fluorescence intensity for populations of cells derived from a patient with bare lymphocyte syndrome. The cells were stained with fluorescently- labeled anti-DR monoclonal antibodies. Figure 10 illustrates the 215 n absorption profile in the final human IL-4 purification step, which consisted of reversed-phase HPLC on a C-4 column. Figure 11 is a construction map of plasmid pEBV-178 containing human IL-4 cDNA. Figure 12 is a construction map of plasmid TRPC11. Figure 13A is a construction map of plasmid pMF-alpha8. Figure 13B illustrates the TCGF activities of several transfection supernatants from yeast cultures expressing native human IL-4 and various muteins thereof. Fiqure 14 depicts growth curves of factor- dependent cell lines; MC/9 mast cells (1A), DX-2 mast cells (IB), NFS-60 cells (IC) and HT2 T cells (ID), 5 x 10 J cells were cultured with varying concentrations of Cl.l supernatant (closed circles), IL-2 R (closed squares), IL-3 R (open circles), GM-CSF R (open triangles), α IFN-γ Λ (closed triangles), or P388D1 supernatant (open diamonds). MC/9 cells ( 1A) were also cultured with varying concentrations of purified IL-3 (closed circles) or IL-3 mixed with 200 units of IL-2 Λ (closed squares), GM-CSF R (open trianqles), IFN-γ R (closed triangles), or P388D1 supernatant (open diamonds). Growth factor activity was measured after 24 hours by colorimetric assay. The absorbance at 570 ran (reference 630 n ) was measured on a Dynatek Micro Elisa reader. Figure 15 illustrates the FPLC cation exchange chromatography of Cl.l supernatant. 7 ml (35 mg protein) of concentrated supernatant was dialyzed into 50 nM Na phosphate, 1 mm EDTA, pH 7.0 (7.8 mS/cm) and applied to Pharmacia Mono S column (0.5 x 5 cm) equilibrated with the same buffer (Buffer A). Buffer B = A + 1M NaCl. Elution conditions: 0.5 ml/min flow rate; 0.5 ml/ fraction; 0-40% B in 40 min, 40-100% B in 10 minutes. Aliquots of each fraction were assayed for proliferation activity on NFS-60 (IL-3, closed circles), HT2 (TCGF, open circles) and MC/9 (MCGF) cells. The MC/9 response is not shown; arrows denote positions were MC/9 proliferation levels reached those of Cl.l supernatant. Figure 16A illustrates reverse phase C8 chromatography of IL-3 depleted Cl.l supernatant. 100 ul of 3X mono-S passed supernatant (fractions 59-61, Fig. 15) in 0.1% TFA was loaded onto Pharmacia C8 reversed phase column (0.5 x 2 cm). Buffer A = 0.1% TFA in H 2 0, Buffer B = 0.1% TFA in acetonitrile. Elution conditions: 0.5 ml/min, 0,5 ml/fraction, 0-25% B in 4 minutes, 25-60% B in 50 minutes, 60-100% 3 in 4 minutes. Aliquots of each fraction were assayed for proliferation activity on NFS-60 (IL-3, closed circles), HT2, (TCGF, open circles) and MC/9 cells (MCGF, closed triangles). Arrow denotes fraction containing peak TCGF activity, and which produced MC/9 proliferation levels similar to those of Cl.l supernatant after addition of saturatinq IL-3 Λ levels to each fraction. Fiqure 16B illustrates reverse phase C8 chromatography of GK15-1 murine T cell supernatant. 2 mg (0.5 ml) of concentrated GK15-1 supernatant in 0.1% TFA was applied to a Pharmacia C8 reverse phase column and eluted as above. Aliquots of each fraction were assayed for TCGF activity on HT2 cells (open circles). Arrow marks the position where murine IL-2 eluted under identical conditions. INSERT: titrations of Cl.l (closed circles) and IL-2 R (closed squares) and GK15-1 supernatant (open circles) TCGF activities directly compared on the same plate. Figure 17 depicts the MCGF and TCGF activity of partially purified factor from reverse phase column (RP factor). Proliferation was measured by [ H]-thymidine incorporation after a 24 hour culture period. MC/9 mast cells and HT2 T cells were cultured with varying concentration of Cl.l supernatant (closed circles), IL-3 (open circles), IL-2 (open triangles), RP factor (open squares) and dilutions of RP factor in the presence of 200 units of IL-3 Λ (closed squares) or dilutions of IL-2 in the presence of saturatinq levels of RP factor (closed triangles) . ^ _ Fiqure 18 illustrates chromatofocussing of Cl.l supernatant. 1.5 ml (3.5 q) of supernatant in 25 nM bis-tris, pH 7.1 was loaded onto a Pharmacia Mono P column (0.5 x 20 cm) and eluted with Polybuffer 74 (1:10), pH 4.0, flow rate 0.5 ml/min, 1 ml/fraction. Aliquots of each fraction were assayed by proliferation on NFS-60 (IL-3, open circles) HT2 (TCGF, closed circles) and MC/9 (MCGF, not shown, except that peak MCGF activity is indicated by arrow). The TCGF peak (fraction 12, pl=6.2) coincides with a minimum IL-3 activity; MCGF proliferation levels are hiqhest at the arrow designation. Figure 19 depicts an SDS PAGE of Cl.l super¬ natant and partially purified MCGF/TCGF. A) Non-reducing SDS PAGE of unfractionated supernatant. Prior reduction with 50 mM DTT (60° x 5 minutes) shifts TCGF peaks to slightly higher molecular weights (21 Kd and 16 Kd) , and is accompanied by a drastic loss in activity. B) Non-reducing SDS PAGE of peak MCGF/TCGF fractions from cation exchange chromatography (Fractions 59-61, Fig. 15). Prior reduction with 50 mM DTT (at 60°C for 5 minutes) shifts MCGF and TCGF peaks to slightly higher molecular weights (21 Kd and 16 Kd) , and is accompanied by a drastic loss in activity. Arrows mark the only fractions to which addition of saturating amounts of IL-3 raises MCGF activity to supernatant levels: IL-3 (open circles), TCGF (closed circles), MCGF (open triangles) . The present invention includes glycosylated or unglycosylated mammalian polypeptides which exhibit IL-4 activity, and which are derivable from the IL-4 polypeptides disclosed herein using standard protein engineering techniques. The invention also includes nucleic acids having sequences capable of coding for the polypeptides of the invention, and nucleic acids whose sequences are e fectively homoloqous to the cDNA clones of the invention. Finally, the invention includes methods of making the glycosylated or unqlycosylated polypeptides of the invention which utilize the nucleotides sequences disclosed herein, and methods of using the polypeptides of the invention. Techniques for making, using, and identifying the polypeptides and nucleic acids of the invention are discussed below in general terms. Afterwards several specific examples are provided wherein the qeneral techniques are applied using specific cell types, vectors, reagents, and the like. Their isolation from natural sources will first be briefly described. These polypeptides can be purified to apparent homogenity from supernatants of readily available cellular sources, and can thus be economically manufactured in pure form, enabling various therapeutic and other utilities. These polypeptides are characterized by including a polypeptide exhibiting B-cell-, T-cell- and mast-cell-stimulatory activity. In natural form in one species, the polypeptides exhibit a molecular weight on SDS PAGE of about 20 kd and 15 kd under non-reducing conditions and 21 kd and 16 kd under reducing conditions. A composition containing the subject polypeptides in substantially pure form exhibits an isoelectric point (pi) of about 6.2 by • • chromato- focussinq. The qrowth factors are extremely potent agents, exhibiting significant activity at extremely low concentrations (e.g., less than 1 nanogram per ml). The polypeptides can be distinguished from hormones and other proteins produced naturally by T cells (e.g., IL-2, IL-3, GM-CSF and IFN-γ) through cation exchanqe chromatoqraphy or unique elution conditions from reverse-phase columns. In murine species, some polypeptides of the present invention support only low levels of proliferation of certain cells, such as IL-3-dependent mast cell lines, but synerqi-stically enhance the qrowth of such cells (e.q., mast cells) in the presence of a second factor (e.g., IL-3). The polypeptides can stimulate the proliferation of several T-cell lines, but generally to a lesser extent than purified IL-2. Also, such polypeptides can induce la expression on resting B- cells and enhance IgG-^ and IgE secretion by B-cells, , similar to 3SF-1. These activities are not separable despite multiple biochemical f actionations. However, most of the activities are destroyed after reduction, e.g., in the presence of SDS. These factors can be obtained from a number of natural sources in a variety of ways. One suitable source is any of a number of T-cell lines that produce the- subject polypeptides. One such T-cell line was derived from C57GL6 mice (Nable, G. et al., Proc. Natl. Acad. Sci. U.S.A., 78: 1157-1161 (1981), which line can be maintained in modified supplemental DME (Nable, G. et al., Cell, 23: 19-28 (1982). This cell line, originally designated Cl.Lyl + 2~/9, was deposited at the American Type Culture Collection and designated accession number CRL 8179. Other suitable cellular sources of these polypeptides include almost any cells that secrete the various antibodies ascribed to the polypeptides, such as human peripheral blood lymphocytes as well as any of the well known T-cell sources, such as tonsils, spleens, etc. The supernatant from such cellular sources or, in some cases, fluid from the disrupted cells, may be subjected to a variety of well-known purification procedures in order to separate the proteins of the present invention. Preferably, the purification techniques are used in combination to ensure high purity levels. Typically, the purifications will utilize gel filtration chromatography, SP cation exchange chroma¬ tography, reverse phase chromatoqraphy,- qroup-speci ic chromatography (e.g., on Heparin Sepharose), or other common separation methods providinq effective protein fractionation. Hiqh pressure liquid chromatography (HPLC), isoelectric focussinq, and SDS polyacrylamide qel electrophoresis may also be utilized. For example, a supernatant from an appropriate cell line may be first fractionated by strong cation exchange chromatography. Typically, the polypeptides of the present invention remain bound to the column, while about 98% of the loaded protein passes through. The remaining bound protein may be eluted with a salt gradient. In one embodiment of the present invention, a sodium chloride gradient released the subject polypeptide at about 0.19 M. This fractionation was repeated twice more (primarily to remove residual IL-3), providing greater than about 95% purity. Thereafter, the material was successively fractionated by Heparin-Sepharose and reverse phase (c4 or cl8) chromatography. In the latter case, these polypeptides eluted at 42% acetonitrile. These pooled fractions contain the subject polypeptide in extremely high purity, essentially to homogeneity (a single band at about 20 kd on silver-stained SDS-PAGE). This purified material is at least 65,000 times more pure than the material as it exists in natural form. These polypeptides may be used as antigens for the production of antibodies, which in turn may be used as antigens for the production of anti-idiotypic antibodies. Either polyclonal or monoclonal antibodies may be prepared in conventional ways. These polypeptides or fragments thereof, generally fragments having at least about 15 amino acids, may be used by themselves, but are preferably bound or linked to an adjuvant or antigen which activates the immune system. Various antigens may be used, such as serum albumins, keyhole limpet hemocyanin, globulins, or the like. A wide variety of techniques are available for linking adjuvants to poly¬ peptides, such as glutaraldehyde, maleimidobenzoic acid, diazobenzoic acid, or the like. Adjuvants include Freund's adjuvant, aluminum hydroxide, or the like. The antigen is injected into an appropriate host in conventional amounts, and one or more booster injections may be made at from 2 to 4 week intervals. Where monoclonal antibodies are employed, normally a mouse is injected with the original and booster injections and the spleen is isolated, and then the splenocytes are fused with an appropriate fusion partner in accordance with conventional techniques. See, for example, Galfre et al., Nature (1977) 266:550; Kennett et al. , Current Topics in Microbiology and Immunology (1978) 81:77; and U.S. Patent Nos. 4,381,292 and 4,363,799. However, for special purposes, other mammals may be employed, such as primates, e.g., humans, for production of antibodies having human F c chains. The polypeptides may be used by themselves or in combination with the antibodies in diagnosis for the polypeptides. Either or both may be labeled or unlabeled for use in diagnostic assays. A large number of diagnostic assays are described in the literature and including binding, either directly or indirectly, these polypeptides or antibodies to a variety of labels, such as enzymes, radionuclides, fluorescers, substrates, coenzymes, particles, e.g., magnetic particles, or the like. As illustrative of these assays see for example U.S. Patents Nos. 3,817,837; 3,850,752; 4,174,384; 4,177,437 and 4,374,925. Various assays are divided arbitrarily into homogeneous and heterogeneous immunoassays, where the distinction is based on whether the complex between the polypeptide and its antibody must be separated from the uncomplexed members of the specific binding pair. Various assays are referred to as El, ELISA, RIA, homogeneous EIA, dot-blot. Westerns, or the like. Antibodies to these polypeptides may be used in themselves as antigens to produce anti-idiotypes, which may serve as competitive antigens, having epitopic sites competitive with epitopic sites of these polypeptides. These anti-idiotypes may therefore serve as tumor inhibitors, as substitutes for the subject polypeptides or as antaqonists for the subject polypeptides. These polypeptides form a family of naturally- occurring polypeptides which may be derived from natural sources, as well as non-naturally occurring polypeptides which share physiological properties, such as binding specificity and B-cell or mast cell stimulatory activity. Minor differences between species or oriqin may require modification in the purification protocols, as known to those skilled in the art. Alternatively, these polypeptides may be sequenced and then fragments synthesized in accordance with well-known techniques. See, for example, Barany and Merrifield, Solid-Phase Peptide Synthesis, "The Peptides, Analysis Synthesis Biology," Special Methods in Peptide Synthesis, Part A, Vol. 2, Gross and Merenhofer, eds.. Academic Press, N.Y. 1980, pp. 1-284. The polypeptide compositions of the present invention will find utility in a variety of ways. For example, these polypeptides can indue the growth of T- cells and mast cells, either in culture or in vivo. Mast cells, as noted previously, are important sources of heparin, histamines, prostaglandins, and other physiologically important materials. Similarly, the growth factors may be utilized to stimulate B- and T- cells, especially those known to secrete proteins (e.g., lymphokines and immunoqlobulins) useful in the immune system. Typically, the factors will be' incorporated into culture medium added to cell cultures. A suitable concentration of the factors will vary depending upon the type of cell line, but will generally be present in about 0.1 ug/ml to about 1 mg/ml, more preferably about 1 ug/ml to about 10 ug/ml, in cultures. The use of the growth- factors of the present invention can eliminate the requirement of utilizing feeder cells to maintain the desired cell lines, resulting in substantial increases in the purity of the products from the cell line. The polypeptide compositions of the present invention will also find use in vivo in the treatment of various immune deficiencies, or to increase the natural immune response. For example, such polypeptide compositions may aid in the treatment or prevention of infection by stimulating the maturation and/or growth of B-cells, T-cells and mast cells, thus decreasing the animal's susceptibility to infection. I. De Novo Preparation of IL-4 cDNA A variety of methods are now available for de novo preparation and cloning of cDNAs, and for the construction of cDNA libraries: e.g. recent reviews are given by Doherty, "Cloning and Expression of cDNA" , Chapter 10 in Gottesman, Ed. Molecular Cell Genetics (John Wiley & Sons, New York, 1985); and Brandis et al., "Preparation of cDNA Libraries and the Detection of Specific Gene Sequences", in Setlow et al., Eds. Genetic Engineering, Vol. 8, pgs. 299-316 (Plenum Press, New York, 1986). By way of example, total mRNA is extracted (e.g., as reported by Berger, S. et al.. Biochemistry 18: 5143-5149 [1979] ) from cells (e.g., a nontransformed human T-cell source) producing polypeptides exhibiting the desired activity. The double-stranded cDNAs from this total mRNA can be constructed by using primer- initiated reverse transcription (Ver e, I., Biochem. Biophys. Acta, Vol. 473, pqs. 1-38 [1977]) to make first the complement of each mRNA sequence, and then by priming for second strand synthesis (Land, H. et al., Nucleic Acids Res., 9: 2251-2266 [1981] ). Subsequently, the cDNAs can be cloned by joininq them to suitable plasmid or bacteriophage vectors (Rougeon, F. et al . , Nucleic Acids Res., 2, 2365-2378 [1975]) or Scherer, G, et al . , Dev. Biol. 86, 438-447 [1981]) through complementary homopolymeric tails (Efstratiadis, A. et al. , Cell, 10, 571-585 [1977]) or cohesive ends created with linker segments containing appropriate restriction sites (Maniatis et al. , Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, N.Y. 1982) , and then transforming a suitable host. (See generally, Efstratiadis, A., and Villa-Kormaroff, L. , "Cloning of double stranded cDNA" in Setlow, J. and Hollaender, A. (eds.) Genetic Engineering, Vol, 1, Plenum Publishing Corp., N.Y., U.S.A. [1982].) A preferred source of mRNA encoding the desired polypeptides is cells whose supernatants contain the stimulating activities for the B-cell, T-cell and/or mast cells, or other activities associated with the polypeptides of the present invention. One such line is the mouse T-cell line Cl.Lyl + 2~/9 (A.T.C.C. Accession No. CRL8179) (Nabel, G. et al. , Nature 291: 332-334 (1981) ) . In general, suitable T-cells can be obtained from a variety of sources, such as mammalian (e.g. human) spleen, tonsils and peripheral blood. T-cell clones, such as those isolated from peripheral blood T- lymphocytes, may also be used (see Research Monographs in Immunology, eds. von Doehmer, H. and Haaf, V.; Section D: "Human T-Cell Clones", vol.8, pgs. 243-333; Elsevier Science Publishers, N.Y. [1985]). Production of mRNAs capable of coding for IL-4 by such cells can be confirmed by microinjeσtion of the extracted mRNA into oocytes of Xenopus laevis. This microinjection technique is described more fully below, and is disclosed generally in Col an et al. , "Export of Proteins from Oocytes of Xenopus Laevis" , Cell, Vol. 17, pgs. 517-526 (1979); and Maniatis et al. Molecular Cloning: A Laboratory Manual, pgs. 350-352 (Cold Spring Harbor Laboratory, New York, 1982) . If the mRNAs coding for a desired IL-4 make up a very small fraction of the total mRNA steps may be necessary to enrich the fractional concentration in order to make practical the screening procedure for detecting cDNA clones of interest. Such procedures are standard in the art and are disclosed in the examples below and in several papers and references, such as Maniatis et al., pgs. 225-228, cited above; Suggs et al. , Proc. Natl. Acad. Sci., Vol. 78, pgs. 6613-6617 (1981); Parnes et al., Proc. Natl. Acad. Sci., Vol. 78, pgs. 2253-2257 (1981), Davis et al., Proc. Natl. Acad. Sci., Vol. 81, pgs. 2194-2198 (1984). A preferred method of de novo preparation of IL-4 cDNAs relies on functional expression of the cDNAs in pcD expression system developed by Okaya a and Berg, disclosed in Mol. Cell. Biol. , Vol. 2, pgs. 161-170 (1982); and Vol. 3. pgs. 280-289 (.1983), and available from Pharmacia (Piscataway, N.J.). The pcD expression vector contains the SV40 early promoter, late splicing junction, and the replication origin. This vector permits expression of cDNA inserts in COS 7 monkey cells which provide T antigen for replication of the pcD plasmid. Screening of cDNA libraries includes transfection of pools of plasmid DNA into COS 7 cells using DEAE-Dextran. Since lymphokines, and in particular IL-4s, are secreted proteins, the supernatants from the transfected cells can be assayed for biological activity after incubation for several days. Positive pools are further divided to identify single cDNA clones which give biological activity after transfection. Briefly, the Okayama and Berg expression vector is constructed as follows. Polyadenylated mRNA is annealed to a polydeoxythymidylic acid (oligo dT) tail attached to the protruding strand of a KpnI-digested ρBR322 plasmid containing the SV40 early promoter region. That is, the entire vector serves as a primer for cDNA synthesis. After cDNA synthesis, 3' polydeoxycytidylate (oliqo dC) tails are attached, followed by Hindlll digestion, which lops off (at a unique Hind111 site) a fragment of the SV40 DNA to which one of the oligo dC tails is attached. The SV40 early promoter remains intact, and fortuitously occurring Hindlll sites of the insert are affected minimally because the hybrid cDNA/RNA is resistant to Hindlll diqestion. A separately constructed Hindlll fragment having a 3' polyquanidylated (oligo dG) tail is annealed to the sticky end left by the Hind III digestion. The vector is circularized and treated with E. coli RNase H, DNA polymerase I, and DNA ligase to replace the RNA strand with DNA. The vectors are cloned in E. coli to form the cDNA library. The SV40 elements permit the vectors to be expressed in eucaryotic cells as well as procaryotic cells, and particularly in mammalian cells, such as COS7 monkey cells or Chinese hamster ovary (CHO) cells. Once the cDNA library in the Okayama/Berg plasmid vector has been completed, the cDNA clones are collected, and random pools checked for the presence of the desired cDNAs by standard procedures, e.g. hybrid selection, detection of antigenic determinants on expressed products, and/or functional assays. Positive pools can then be probed with a cDNA from an induced T cell line. Thereafter, the positive, probed pools are divided into individual clones which are further tested by transfection into a suitable host (such as a mammalian cell culture), and the host supernatant is assayed for activity. II. Preparation of IL-4 cDNAs Via Hybridization Probes Derived from Disclosed cDNAs The cDNAs disclosed herein can be used as probes to identify homologous sequences in different cell types, as an alternative to de novo isolation and cloning of the IL-4 coding nucleotides. Standard techniques are employed, e.g. Beltz et al. , "Isolation of Multigene Families and Determination of Homologies by Filter Hybridization Methods," Methods in Enzy ology, Vol. 100, pgs. 266-285 (1983); and Callahan et al., "Detection and Cloning of Human DNA Sequences Related to the Mouse Mammary Tumor Virus Genome," Proc. Natl. Acad. Sci., Vol. 79, pgs. 5503-5507 (1982). Basically, the cDNAs of the invention are .used to construct probes (using standard techniques, e.g. see Maniatis et al., cited above) for screening, at low hybridization stringencies, genomic or cDNA libraries (again, constructed by standard techniques) of a cell type suspected of producing IL-4. Standard screening procedures are employed, e.g. Grunstein et al. , Proc. Natl. Acad. Sci. , Vol. 72, pgs. 3961-3965 (1975); or Benton et al. , Science, Vol. 196, pgs. 180-183 (1977). As described more fully below, human IL-4 was isolated by a murine IL-4 probe. Subsequent analysis indicated about 70% homology between selected regions of the human and mouse cDNAs. Given the evolutionary distance between mice and humans it is believed that most, if not all, mammalian IL-4 genes are detectable by probes constructed from one or more cDNAs of the invention: Wilson et. al. "3iochemical Evolution", Ann. Rev. Biochem. , Vol. 46, pgs. 573-639 (1977); Ki ura, "The Neutral Theory of Molecular Evolution," Chapter 11 in Nei and Koehn, Eds. Evolution of Genes and Proteins (Sinauer Associates, Sunderland, MA, 1983). Ill. Preparation of Mutant IL-4s by Protein Engineering Once nucleic acid sequence and/or amino acid sequence information is available for a native protein a variety of techniques become available for producing virtually any mutation in the native sequence. Shortle, in Science, Vol. 229, pgs. 1193-1201 (1985), reviews techniques for mutating nucleic acids which are applicable to the present invention. Preferably, mutants of the native IL-4s, i.e. IL-4 muteins, are produced by site-specific oligonucleotide-directed mutagenesis, e.g. Zoller and Smith, Methods in Enzy ology, Vol. 100, pgs. 468-500 (1983); Mark et al., U.S. Patent 4,518,584 entitled "Human Recombinant Interleukin-2 Muteins"; or by so-called "cassette" mutagenesis described by Wells et al., in Gene, .Vol. 34, pgs. 315-323 (1985); and Estell et al.. Science, Vol. 233, pgs. 659-663 (1986). In sections below, the notation used by Estell et al. (cited above) to identify muteins is followed and generalized. For example, "human IL-4 mutein Leu 82 " (or simply "Leu 82 " if the native protein is understood from the context) indicates a polypeptide whose amino acid seguence is identical to that of the native protein except for position 82 with respect to the N-terminus. At that position Leu has been substituted for Phe. More than one substitution can be similarly indicated; e.g., a mutein having Leu substituted for Phe at position 82 and Asp for Asn at position 111 is referred to as human IL-4 mutein (Leu 82 , Asp 111 ). Deletions are indicated by "Δ's". For example, a mutein lackinq Gin at position 71 is referred to as human IL-4 mutein Δ . An insertion is indicated by "IS(Xaa)". For example, a mutein with a Leu inserted after Gin at position 71 is referred to as human IL-4 mutein IS 71 (Leu). Thus, human IL-4 mutein (Ser , Δ , represents the native human IL-4 sequence which has been modified by replacing Thr by Ser at position 13, deleting Gin at position 71, and inserting Gly immediately after Ala at position 94. Insertion of multiple amino acids at the same site is indicated by ) , where is the sequence inserted after position i. N-terminal additions are indicated by superscript "0", e.g. IS ϋ (Xaa), and a sequence of deletions, for example of amino acids 6-10, is desiqnated either as Δ δ-1 ° or as (Δ , Δ , A , A , A 10 ). Most preferably cassette mutagenesis is employed to qenerate human IL-4 muteins. As described more fully below, a synthetic human IL-4 gene has been constructed with a sequence of unique restriction endonuclease sites spaced approximately uniformly along the gene. The uniqueness of the restriction sites should be retained when the gene is inserted into an appropriate vector, so that seqments of the gene can be conveniently excised and replaced with synthetic oligonucleotides (i.e. "cassettes") which code for desired muteins. Determination of the number and distribution of unique restriction sites entails the consideration of several factors including (1) preexisting restriction sites in the expression vector, (2) whether species- specific or genera-specific codon usage is desired, and (3) the convenience and reliability of synthesizing and/or sequencing the segments between the unique restriction sites. IV. Biological Properties and Assays for IL-4 Activity. Mammalian IL-4 of the invention is defined in terms of biological activities and/or homology with the disclosed embodiments. Mammalian IL-4s of the invention include proteins and muteins (of the disclosed native polypeptides) which are homologous to the disclosed native polypeptides and which exhibit both BCGF activity and TCGF activity. Mammalian IL-4s of the invention are alternatively defined by their biological activities (defined more fully below) which include BCGF activity and TCGF activity (which is referred to herein as IL-4 activity) as well as at least one or more activities selected from the qroup of activities consisting of MHC antigen induction activity, Fc-epsilon receptor induction activity, GM-CSF stimulated granulocyte colony growth potentiating activity, interleukin-2 TCGF potentiating activity, and I G^- and IgE-induction activity. It is believed that IL-4s are species-specific in their activities. That is, for example, human IL-4 exhibits TCGF activity as assayed by human T cell lines, but not as assayed by murine T cell lines. And conversely, murine IL-4 exhibits TCGF activity as assayed by murine T cell lines, but not as assayed by human T cell lines. A. TCGF Activity Several standard assays have been described for TCGF activity, e.g. Devos et al. , Nucleic Acids Research, Vol. 11, pgs. 4307-4323 (1983); Thur an et al. , J. Biol. Response Modifiers, Vol. 5, pgs 85-107 (1986); and Robert-Guroff et al. , Chapter 9 in Guroff, Ed. Growth and Maturation Factors (John Wiley, New York, 1984) . Generally, the TCGF assays are based on the ability of a factor to promote the proliferation of peripheral T lymphocytes or IL-2 dependent T cell lines, e.g. Gillis et al. J. Immunol., Vol. 120, pg. 2027 (1978). Proliferation can be determined by standard techniques, e.g. tritiated thy idine incorporation, or by colorimetric methods, Mosmann, J. Immunol. Meth. , Vol. 65, pgs. 55-63 (1983). By way of example, human TCGF activity can be assayed by the following steps: (1) washed human peripheral blood lymphocytes (about 2 x 10 in 50 microliters) previously stimulated with phytohemag- glutinin (PHA) for 7 days and subsequently cultured for 7 days with IL-2 are added to a miσrotiter well; (2) dilutions (50 microliter) of the TCGF-containing material are added to each well; (3) the lymphocytes are incubated 72 hours at 37°C; (4) tritiated thymidine (about 20 microliters, 20 microcuries/ml) is added to each well; and (5) cells are harvested onto filter paper strips, washed, and counted in a scintillation counter. As described more fully in the examples, some forms of IL-4 have the capability of potentiating the TCGF activity of IL-2. "Potentiation" as used herein in reference to such activity means that the maximal level of proliferation in a TCGF assay system caused by IL-2 is increased by the addition of IL-4. B. BCGF Activity BCGF activity is defined by an assay disclosed by Howard et al., J. Exp. Med, Vol. 155, pgs. 914-923 (1982). Assays for BCGF are reviewed generally by Howard and Paul, in Ann. Rev. Immunol., Vol. 1, pqs. 307-333 (1983). Briefly, BCGF activity is measured by the degree to which purified resting B cells are stimulated to proliferate in the presence of a submitogenic concentra¬ tion of anti-IgM, or like antigen. By way of example, assay of human BCGF activity can be carried out by the following steps: Enriched B cell populations are obtained from peripheral blood, spleen, tonsils, or other standard sources by Ficoll/Hypaque density gradient centri ugation (e.g. Pharmacia) and two cycles of rosettinq with 2- aminoethylisothiouronium bromide-treated sheep erythrocytes to eliminate T cells. Such B cell preparations should contain more than 95% surface Ig cells and more than 95% cells positive for human B-cell specific antigen, as determined by the anti-human B-cell specific monoclonal antibody Bl available from Coulter (Hialeah, FL). T cell contamination should be less than 1% as determined by staining with anti-Leu-1 monoclonal antibodies (Becton-Dickinson, Mountain View, CA) or OKT 11 antibodies (Ortho Diagnostics, Westwood, MA). 3 milliliter cultures of such B lymphocytes (about 5 x 10 5 per ml in Yssel's medium, Yssel et al., J. Immunol. Meth. , Vol. 65, pgs. 55-63 (1984)), are activated by either Staphylococcus aureus Cowan I strain (SAC) (e.g. 0.01% solution of SAC, which is available from Calbiochem under the tradename Pansorbin, or which can be prepared as described by Falkoff et al. , J. Immunol. , Vol. 129, pg. 97-102 (1982)) or anti-IgM antibodies (e.g. BRL, Gaithersburg, MD) coupled to beads, e.g. 5 microgram/ml of Immunobeads available from Bio-Rad (Richmond, CA) . The B cells are cultured either for 24 hours (in the case of SAC) or 72 hours (for anti-IgM beads) and then repurified by Ficoll/Hypaque density centrifugation to remove SAC particles, beads, nonviable cells, and the like. B cell proliferation is measured by plating about 5 x 10 4 B lymphocytes in 50 microliters of medium in 0.2 ml flat-bottomed microtiter wells. Various dilutions of the materials suspected of having BCGF activity are added in a final volume of 50 microliters. Tritiated thymidine incorporation is determined after 48 hours (anti-IgM cultures) or 72 hours (SAC cultures) . Similar assays are also disclosed by Muraguchi et al. , J. Immunol. , Vol. 129, pgs. 1104-1108 (1982); and Yoshizaki et al. , J. Immunol. , Vol. 128, pgs. 1296-1301 (1981). C. MHC Antigen Induction. It has been demonstrated that IL-4 can induce the expression of MHC antigens (e.g., la in mice) in various cell types of the immune system, particularly B cells. Roeh et al. , in J. Exp. Med. , Vol. 160, pgs. 679-694, presented evidence that a factor exhibiting BCGF activity was also capable of inducing the expression of MHC antigens on normal resting B cells. Assays for MHC antigen induction are generalizations of the assays for murine B cells presented in that reference. Briefly, immune system cells are exposed to IL-4, and then expression of particular MHC antigens on the cells' surfaces are determined by labeled antibodies specific for that antigen. The degree of induction is determined by comparison of the induced cells with controls. Several different antibodies can be employed for any given species. Several hybridomas are available from the ATCC which produce monoclonal anti-MHC antigen antibodies, and several are available commercially (for example, anti-HLA-DR produced by hybridomas under ATCC accession numbers HB103, HB109, HB110, or HB151; anti- Z-&p ' <* produced by hybridoma under ATCC accession number HB35; anti-HLA-DR L243 available from Becton Dickinson (Mountain View, CA); or the like). Some routine experimentation may be required to adapt the assay to a particular species, and to optimize conditions to qive the most sensitive read-out of MHC antigen levels. For the human MHC antigen induction assay, purified B cells can be prepared as described above, or by similar techniques. Alternatively, MHC induction can be assayed on unpurified preparations of spleen cells. Antibody- labeled cells are preferably detected by flow cytometry, e.g. on a Becton Dickinson FACS-type instrument, or the equivalent. D. MCGF Activity It is believed that IL-4s generally exhibit MCGF activity. However, because of the lack of adequate assay techniques, MCGF activity has only been demonstrated for rodent IL-4. Murine IL-4 MCGF assays are based on the proliferation of factor-dependent murine mast or basophil cell lines. In particular, MCGF activity can be assayed with the murine mast cell line MC/9, which is deposited with the ATCC under accession number CRL 8306 and is described in U.S. Patent 4,559,310 and in Nabel et al.. Cell, Vol. 23, pg. 19 (1981). Murine MCGF assays are also described by Ihle et al . , in J. Immunol., Vol. 127, pg. 794 (1981). Preferably MCGF activity is determined by the colorimetric assay of Mosmann (cited above) with the use of MC/9 cells. Briefly, MC/9 cells are cultured in flat- bottom Falcon microtiter trays (10 cells/well) in Dulbecco's modified medium supplemented with 4% fetal calf serum, 50 uM 2-mercaptoethanol, 2 mM glutamine, nonessential amino acids, essential vitamins, and varied concentrations of test supernatants in a final volume of 0.1 ml. Fifty microqrams of 3-(4, 5-dimethylthiazol-2- yl )-2,5-diphenyl tetrazolium bromide (Siqma) in 10 ul of phosphate-buffered saline were added to each cell culture after a 20-hr incubation. Four hours later, 0.1 ml of 0.04 M HC1 in isopropanol was added to solubilize the colored formazan reaction product. The absorbance at 570 nm (reference 630 nm) is measured on a Dynatek Microelisa Autoreader (MR580), or similar instrument. E. Fc-epsilon Receptor Induction. It has been discovered that IL-4 induces Fc- epsilon expression on B cells and on T cells, but particularly on human B cells stimulated by anti-IgM antibodies, or like antigen. It has also been discovered that gamma interferon specifically inhibits IL-4-induced Fc-epsilon expression on B cells. Preferably, the assay for Fc-epsilon receptor induction proceeds initially as for the BCGF assay. That is, purified B cells are obtained which are then stimulated with anti-IgM antibody (or like antigen) and are exposed to IL-4. Finally the ceils are assayed for Fc-epsilon receptors. Several assays are available for quantifying Fc-epsilon receptors on cell surfaces, e.g. Yodoi and Ishizaka, J. Immunol., Vol. 122, pgs. 2577-2583 (1979); Hudak et al., J. Immunol Meth., Vol. 84, pgs. 11-24 (1985); and Bonnefoy et al. , J. Immunol. Meth. , Vol. 88, pqs. 25-32 (1986). In particular, Fc-epsilon receptors can be measured by flow cytometry with labeled monoclonal antibodies specific for the receptors, e.g. using a Becton Dickinson FACS-IV, or like instrument. Fc-epsilon receptor specific monoclonals can be constructed using conventional techniques. F. lgG- j _- and IgE-Induction. IL-4 induces the secretion of IgE and IgG ] _ isotypes in lipopolysaccharide (LPS)-activated B cells, e.g. Coff an et al. , J. Immunol. , Vol. 136, pgs. 4533- 4541 (1986); Sideras, et al., Eur. J. Immunol. , Vol. 15, pgs. 586-593 (1985) . These activities can be measured by standard immunoassays for antibody isotype, as described by Coffman et al. , J. Immunol. , Vol. 136, pgs. 949-954 (1986) . Briefly, B cells are LPS-activated by culturing them with, for example, about 4 micrograms/ l of Salmonella typhimurium LPS (available from Sigma) or about 50 microgram/ l LPS extracted from E. coli 055 (as described by Sideras et al., cited above). After 4 - 8 days' culture, supernatants are harvested for assaying. Standard isotype-specific ELISA-type assays can be used to measure the various isotype concentrations. Anti- isotype antibodies for the assay are available commercially, or can be obtained from the ATCC. G. Colony Stimulating Factor (CSF) Activity. To determine CSF activity, hemopoietic cells, e.g. bone marrow cells or fetal cord blood cells, are made into a single cell suspension. The individual cells are then "immobilized" in a semi-solid (agar) or viscous (methylcellulose) medium containing nutrients and usually fetal calf serum. In the presence of an appropriate stimulating factor, individual cells will proliferate and differentiate. Since the initial cells are immobilized, colonies develop as the cells proliferate and mature. These colonies can be scored after 7-14 days, 3urgess, A. , Growth Factors and Stem Cells, pgs. 52-55, Academic Press, New York [1984]. (For specific application to the growth of granulocytes and macrophaqes, see Bradely, T. and Metcalf, D. , Aust. J. Exp. Biol. Med. Sci. Vol. 44, pgs. 287-300 [1966], and see generally Metcalf, D. , Hemopoietic Colonies, Springer-Verlag, Berlin [1977] ) . If desired, individual colonies can be extracted, placed on microscope slides, fixed and stained with Wright/Geimsa (Todd-Sanford, Clinical Diagnosis by Laboratory Methods, 15th Edition, Eds. Davidson and Henry [1974]). Morphological analysis of cell types present per single colony can then be determined. Bone marrow cells collected from patients with nonhe atologic disease are layered over Ficoll (type 400, Sigma Chemical Co., St. Louis, MO), centrifuged (2,000 rpm, 20 in), and the cells at the interface removed.- These cells are washed twice in Iscove's Modified Dulbecco's Medium containing 10% fetal calf serum (FCS) , resuspended in the same medium and the adherent cells removed by adherence to plastic Petri dishes. The nonadherent cells are added at 10 5 cells/ml to Iscove's Medium containinq 20% FCS, 50 .M 2-mercaptoethanol, 0.9% methylcellulose and varied concentrations of either supernatants known to contain colony stimulating activity or test supernatants. One ml aliquots are plated in 35 mm petri dishes and cultured at 37°C in a fully humidified atmosphere of 6% C0 2 in air. Three days after the initiation of the culture, 1 unit of erythropoietin is added to each plate. Granulocyte-macrophage colonies and erythroid bursts are scored at 10-14 days using an inverted microscope. Cord blood cells collected in heparin are spun at 2,000 rpm for 6 min. The white blood cells at the interface between the plasma and red blood cell peak are transferred to a tube containing 0.17 N ammonium chloride and 6% FCS. After 5 min on ice, the suspension is underlaid with 4 ml FCS and centrifuged for 6 ins at 2,000 rpm. The cell pellet is washed with Dulbecco 1 s phosphate buffered saline and put through the Ficoll and plastic adherence steps as described above for bone marrow cells. The low density nonadherent cells are collected and placed at 10 cells/culture in the semi- solid culture medium as described above. At the end of the assays, the cellular composition is determined after applying the individual colonies to glass slides and staining with Wright- Giemsa. Eosinophils are determined by staining with Luxol Fast Blue (Johnson, G. and Metcalf, D. , Exp. Hematol. Vol. 8, pgs. 549-561 [1980]). "Potentiation" as used herein in reference to GM-CSF-stimulated granulocyte growth means that granulocyte colonies in the assays described above are larger when GM-CSF is used with IL-4 than when GM-CSF is used alone to stimulate colony growth. V. Purification and Pharmaceutical Compositions The polypeptides of the present invention expressed in E. coli, in yeast or in other cells can be purified according to standard procedures of the art, including ammonium sulfate precipitation, fraσtionation column chromatography (e.g., ion exchange, gel filtration, electrophoresis, affinity chromatography, etc.) and ultimately crystallization (see generally "Enzyme Purification and Related Techniques,"Methods in Enzymoloqy, 22: 233-577 [1977], and Scopes, R., Protein Purification: Principles and Practice, Springer-Verlag, New York [1982]). Once purified, partially or to homogeneity, the polypeptides of the invention may be used for research purposes, e.g., as a supplement to cell growth media (e.g., minimum essential medium Eagle, Iscove's modified Dulbecco Medium or RPMI 1640; available from Sigma Chemical Company (St. Louis, MO) and GI3C0 Division (Chagrin Falls, OH)) and as an antigenic substance for eliciting specific i munoglobulins useful in immunoassays, immunofluorescent stainings, etc. (See generally Immunological Methods, Vols. I & II, Eds. Lefkovits, I. and Pernis, B., Academic Press, New York, N.Y. [1979 & 1981]; and Handbook of Experimental Immunology, ed. Weir, D., Blackwell Scientific Publications, St. Louis, MO [1978].) The polypeptides of the present invention may also be used in pharmaceutical compositions, e.g., to enhance natural defense against various infections. Thus, patients with rheumatoid arthritis, in need of a transplant, or with immunodeficiency caused by cancer chemotherapy, advanced age, immunosuppressive agents, etc., may be treated with such polypeptides. The compositions can selectively stimulate various components of the immune system, either alone or with other agents well known to those skilled in the art. In particular, the compositions may include other immune-reactive agents, such as lymphokines (e.g. IL-1, IL-2, etc.), any of the colony stimulating factors, immunoglobulins, etc., in view of the potentiating activities of the polypeptides of the present invention. The polypeptides will also find use in situations (in vivo or in vitro) in which enhanced cellular proliferation or immunoglobulin production is desired. Pharmaceutical compositions of this invention can be prepared by mixing these polypeptides with preferably inert, pharmaceutically acceptable carriers. Suitable carriers and processes for their preparation are well known in the art (see, e.g., Remington' s Pharmaceutical Sciences and U.S. Pharmacopeia: National Formulary, Mack Publishing Company, Easton, PA [1984]). The preferred course of administration is parenteral and can include use of mechanical delivery systems. Preferably, the pharmaceutical composition is in unit dosage form, each unit containing an appropriate quantity of the active component. The quantity of active compound in a unit dose of preparation may be varied or adjusted from 1 ug to 100 mg, according to the particular application and the potency of the active ingredient. The composition can, if desired, also contain other therapeutic agents. The dosages may be varied depending upon the requirement of the patient, the severity of the condition being treated and the particular compound being employed. The term "effective amount" as used herein is meant to take these factors into account when dosages are considered. Determination of the proper dosage for a particular situation is within the skill of the art. Generally, treatment is initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage is increased by small amounts until the optimum effect under the circumstances is reached. For convenience, the total daily dosage may be divided and administered in portions during the day. VI. Expression Systems A wide range of expression systems (i.e. host- vector combinations) can be used to produce the proteins and muteins of the present invention. Possible types of host cells include but are not limited to cells from bacteria, yeast, insects, mammals, and the like. Optimizing the expression of a particular protein or mutein depends on many factors, including (1) the nature of the protein or mutein to be expressed, e.q. the expressed product may be poisonous to some host systems, (2) whether, and what type of, post- translational modifications are desired, e.g. the extent and kind of qlycosylation desired may affect the choice of host, (3) the nature of the 5' and 3' regions flanking the coding region of the protein or mutein of interest, e.q. selection of promoters and/or sequences involved in the control of translation is crucial for efficient expression, (4) whether transient or stable expression is sought, (5) the ease with which the expressed product can be separated from the proteins and other materials of the host cells and/or culture medium, (6) the ease and efficiency of transfecting hosts which transiently express the protein or mutein of interest, (7) the scale of cell culture employed to express the protein or mutein of interest, (8) whether the protein or mutein of interest is expressed fused to a fragment of protein endogenous to the host, and like factors. In general prokaryotes are preferred for cloninq the DNA sequences of the invention. General guides for implementing prokaryotic expression systems are provided by Maniatis et al. , Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, N.Y., 1982); Perbal, A Practical Guide to Molecular Cloninq (John Wiley & Sons, N.Y. , 1984); Glover, DNA Cloninq: A Practical Approach, Vol. I and II (IRL Press, Oxford, 1985); and de Boer et al. , "Strategies for Optimizing Foreign Gene Expression in Escherichia coli," in Genes: Structure and Expression, Kroon, ed. (John Wiley & Sons, N.Y., 1983). For example, E. coli K12 strain 294 (ATCC No. 31446) is particularly useful. Other microbial strains which may be used include E. coli strains such as E. coli B, and E. coli X1776 (ATCC No. 31537). These examples are, of course, intended to be illustrative rather than limiting. Prokaryotes may also be used for expression. The aforementioned strains, as well as E. coli W3110 (Fs~, λ~, prototrophic, ATCC No. 27325), bacilli such as Bacillus subtilis, and other enterobacteriaceae such as Salmonella typhimurium or Serratia marcesans, and various pseudomonas species may be used. In general, plasmid vectors containinq replicon and control sequences which are derived from species compatible with the host cell are used in connection with these hosts. The vector ordinarily carries a replication site, as well as marking sequences which are capable of providing phenotypic selection in transformed cells. For example, E. coli is frequently transformed using pBR322, a plasmid derived from an E. coli species (Bolivar, et al., Gene Vol. 2, pg. 95 (1977)). pBR322 contains genes for ampicillin and tetracycline resistance and thus provides easy means for identifying transformed cells. The pBR322 plasmid or other microbial plasmid must also contain, or be modified to contain, promoters which can be used by the microbial organism for expression of its own proteins. Those promoters most commonly used in recombinant DNA construction include the 3-lactamase (penicillinase) and lactose promoter systems (Chang et al. Nature, Vol. 275, pg. 615 (1978); Itakura, et al. Science, Vol. 198, pg. 1056 (1977); Goeddel, et al, Nature Vol. 281, pg. 544 (1979)) and a tryptophan (trp) promoter system (Goeddel, et al, Nucleic Acids Res., Vol. 8, pg. 4057 (1980); EPO Appl. Publ. No. 0036776). While these are the most commonly used, other microbial promoters have been discovered and utilized, and details concerning their nucleotide sequences have been published, enabling a skilled worker to ligate them functionally with plasmid vectors (Siebenlist et al , Cell Vol. 20, pq. 269 (1980)). In addition to prokaryotes, eukaryotic microbes, such as yeast cultures, may also be used. Saccharomyces cerevisiae, or common baker's yeast, is the most commonly used amonq eukaryotic microorganisms. For expression in Saccharomyces, a commonly-used plasmid is YRp7 (Stinchco b, et al, Nature, Vol. 232, oq 39 (1979); Kinqsman et al , Gene , Vol. 7, pq. 141 (1979); Tschemcer, et al, Gene, Vol. 10, pg. 157 (1980)). This plasmid already contains the trpl gene which provides a selection marker for a mutant strain of yeast lacking the ability to grow in tryptophan, for example ATCC No. 44076 or PΞP4-1 (Jones, Genetics, Vol. 85, pg. 12 (1977)). The presence of the trpl lesion as a characteristic of the yeast host cell genome then provides an effective environment for detecting transformation by growth in the absence of tryptophan. Suitable promoting sequences in yeast vectors include the promoters for 3-phosphoglyσerate kinase (Hitzeman, et al. , J. Biol. Chem. , Vol. 255, pg. 2073 (1980)) or other glycolytic enzymes (Hess, et al, J. Adv. Enzyme Reg. , Vol. 7, pg. 149 (1968) ; Holland, et al. Biochemistry, Vol. 17, pg. 4900 (1978)), such as enolase, glyceraldehyde-3-phosphate dehydrogenase, hexokinase, pyruvate decarboxylase, phosphofructo inase, glucose-6- phosphate isomerase, 3-phosphoglycerate mutase., pyruvate kinase, triosephosphate isomerase, phosphoglucose isomerase, and glucokinase. In constructing suitable expression plasmids, the termination sequences associated with these genes are also ligated into the expression vector at a position 3' of the sequence to be expressed, to provide polyadenylation of the mRNA and termination. Other promoters, which have the additional advantage of transcription controlled by growth conditions, are the promoter regions for alcohol dehydrogenase 2, isocytochrome C, acid phosphatase, degradative enzymes associated with nitrogen metabolism, the aforementioned glyceraldehyde-3-phosphate dehydrogenase, and enzymes responsible for maltose and galactose utilization (Holland, ibid.) . Any plasmid vector containing a yeast- compatible promoter, origin of replication and termination sequences is suitable. In addition to microorganisms, cultures of cells derived from multicellular organisms may also be used as hosts. In principle, any such cell culture is workable, whether vertebrate or invertebrate. However interest has been greatest in vertebrate cells, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure in recent years [Tissue Culture, Academic Press, Kruse and Patterson, editors (1973)]. Examples of such useful host cell lines are VERO and HeLa cells, Chinese hamster ovary (CHO) cell lines, and W138, BHK, C0S7, mouse myeloma (ATCC No. TIB 19 or TIB 20), and MDCK cell lines. Expression vectors for such cells ordinarily include (if necessary) an origin of replication and a promoter located in front of the gene to be expressed, along with any necessary ribosome binding sites, RNA splice sites, polyadenylation site, and transcriptional terminator sequences. For use in mammalian cells, the control functions on the expression vectors are often provided by viral material. For example, commonly used promoters are derived from polyoma, Adenovirus 2, and most frequently Simian Virus 40 (SV40) . The early and late promoters of SV40 virus are particularly useful because both are obtained easily from the virus as a fragment which also contains the SV40 viral origin of replication (Fiers, et al. Nature, Vol. 273, pg 113 (1978). Smaller or larger SV40 fragments may also be used, provided that there is included the approximately 250 bp sequence extending from the Hindlll site toward the B ll site located in the viral origin of replication. Further, it is also possible, and often desirable, to utilize promoter or control sequences normally associated with the desired gene sequence, provided that such control sequences are compatible with the host cell systems. An origin of replication may be provided either by an exogenous origin on the vector, e.g., on one derived from SV40 or other viral (e.g. Polyoma, Adeno, VSV, 3PV, etc.) source, or endogeneously by the host cell chromosomal replication mechanism. It is often sufficient to integrate the vector into the host cell chromosome. In selecting a preferred host cell for transfection by the vectors of the invention which comprise DNA sequences encoding both t-PA and DHFR protein, it is appropriate to select the host according to the type of DHFR protein employed. If wild type DHFR protein is employed, it is preferable to select a host cell which is deficient in DHFR, thus permitting the use of the DHFR coding sequence as a marker for successful transfection in selective medium which lacks hypoxanthine, glycine, and thymidine. Such a appropriate host cell is the Chinese hamster ovary (CHO) cell line deficient in DHFR activity, prepared and propagated as described by ϋrlaub and Chasin, Proc. Natl. Acad. Sci. (USA) Vol. 77, pg. 4216 (1980). On the other hand, if DHFR protein with low binding affinity for MTX is used as the controlling sequence, it is not necessary to use DHFR-resistant cells. Because the mutant DHFR is resistant to methotrexate, MTX-containing media can be used as a means of selection provided that the host cells are themselves methotrexate-sensitive. Most eukaryotic cells which are capable of absorbing MTX appear to be methotrexate- sensitive. One such useful cell line is a CHO line, CHO- Kl ATCC No. CCL 61. Invertebrate expression systems include the larvae of silk worm, Bo byx ori, infected by a baculovirus vector, BmNPV, described by Maeda et al. in Nature, Vol. 315, pgs. 892-894 (1985), and in Saibo Koguku, Vol. 4, pgs. 767-779 (1985). EXA PLES The following examples serve to illustrate the present invention. Selection of vectors and hosts as well as the concentration of reagents, temperatures, and the values of other variables are only to exemplify application of the present invention and are not to be considered limitations thereof. MATERIALS AND METHODS T Cell lines Cl.Lyl + 2 " /9 (referred to as Cl.l), Nabel, G. , Greenberqer, J.S., Sakakeeny, M.A. and Cantor, H. (1981) Proc. Natl. Acad. Sci. USA 78, 1157-1161, and GK15-1 (supplied by M. Giedlin, DNAX Research Institute of Molecular and Cellular Biology, Inc.; DNAX) were resuspended at 5 x 10 5 cells/ml in RPMI 1640, 50um 2ME, 1% FCS and 2yg/ml Con A for 24 hours. The supernatants were collected and stored at -70°C. A cloned mast cell line, MC/9 (Nable, G. , Galli, S.J., Dvorak, H.F. and Cantor, H. (1981) Nature 291, 332-334), was obtained from G. Nabel (Dana Farber Cancer Institute; DFCI). The mast cell lines MM3 (provided by R. Coffman, DNAX) and Dx-2 (derived by D. Rennick, DNAX) were characterized by the absence of myelomonocytic-associated markers and by the presence of IqE receptors and histamine levels greater than 250 nq/10 5 cells. The yeloid NFS-60 cell line was provided by J. Ihle (Frederick Cancer Research Facility; FCRF ) . The NFS-60 cell line was subcloned to obtain an IL-3- dependent clone. The T cell line HT2 was obtained from S. Strober (Stanford University, Palo Alto, CA); CTLL-2 was supplied by W. Farrar (FCRF); and Ly 23/4 was supplied by G. Nabel (DFCI). The mast cell and T cell lines were qrown in RPMI 1640, 10% FCS, 50uM 2ME supplemented with recombinant IL-3 or IL-2. Histamine levels of several IL-3-dependent cell lines were assayed usinq the method of Shore, P.A., Burkhalter, A., and Conn, V.H. (1959) J. Pharm. Exp. Ther. 127, 182-186. T Cell and mast cell growth factor activities were determined by [ H]-thymidine incorporation or by a colorimetric assay as described by Mosmann, T. in J. Immunol. Methods 65: 55-63 (1983). IL-3 purified from WEHI 3 supernatant was a gift of J. Ihle (FCRF). Recombinant IL-2, IL-3 , GM-CSF and INF-γ were utilized in the form of supernatant from COS-7 monkey kidney cells transfected with the corresponding cDNA clones. One unit of IL-2, IL-3 or GM-CSF was defined as that amount of factor which stimulated 50% maximum [ H]-thymidine incorporation of factor-dependent cell lines (Rennick, D. et al. (1985) J. Immunol. 134: 910-919). One unit of IFN-γ protects 50% of murine L cells from the cytopathic effect of vesicular stomatitis virus (Schreiber, R. et al. (1982) J. Exp. Med. 156: 677-689). B-cells were prepared from a single cell suspension of mouse spleen cells by depleting T-cells and macrophaqes according to standard procedures (Howard, M. et al., J. Exp. Med. 155: 914 (1982)). The purified factor described herein was assessed for its ability to induce la expression on B-cells usinq cyto luorometric analysis and to stimulate B-cell proliferation using an anti-Ig co-stimulation assay as described by Roehm, N. et al., J. Exp. Med. 160: 679. Protein determinations were based either on UV absorption (280 nm or 220 nm) or standard curves constructed using a dye binding assay (Bradford, M. (1976) Annal. Biochem. 72: 248-254). Supernatants were initially concentrated 20 fold with a Pellicon cassette unit (Millipore, Bedford, MA), or (following reverse phase chromatography) by solvent evaporation in a Speedvac (Savant, Farmingdale, NY). SDS PAGE was perfor ed usinq the Laemmli system ( aemmli , U. (1970) Nature 227: 680-685) with a 12% separating gel. Marker proteins were Pharmacia low MW standards (Pharmacia, Uppsala, Sweden). Gels were silver-stained as described by Merril, C. et al . (1981) Science 211: 1437-1438. To assess directly MCGF and TCGF activity as a function of molecular weight, samples with or without prior reduction with 50 mM DTT were electrophoresed, the gels sliced to 1 mm sections and crushed, and protein was eluted overniqht at 4°C into 0.5 ml assay media supplemented with 5 mq/ml ovalbumin (Sigma, St. Louis, MO). Chromatography was performed at 18°C on a Pharmacia FPLC system equipped with a Kratos Spectroflow 773 UV detector (Kratos, Ramsey, NJ). Cation exchange chromatography utilized a Pharmacia Mono S column (0.5 x 5 cm), equilibrated with 50 mM sodium phosphate, 1 M EDTA, pH 7.0. Supernatants in the same buffer were applied to the column and eluted with a NaCl gradient to 1M. For reverse phase chromatography, a Pharmacia C8 column (0.5 x 2 cm) was used. The sample, diluted with 0.1% TFA to pH 2 , was loaded onto the column and eluted with acetonitrile gradients containing 0.1% TFA. Isoelectric points of MCGF and TCGF activities were estimated by chromatofocussing on a Pharmacia Mono P column (0.5 x 20 cm). The sample, in 0.025 M bis tris, pH 7.1, was loaded onto the "Mono P" column equilibrated with the same buffer. Gradient elution was effected with Pharmacia Polybuffer 74, pH 4.0 (1:10 dilution, pH adjusted with 0.2 M aminodiacetic acid). Effluent pH was continuously determined with a Pharmacia pH monitor. Prior to injections, all samples were filtered throuqh a Millex GV 0.2 U unit (Millipore). Fractions from SDS PAGE or chromatographic runs were assayed for their ability to support proliferation of three cell lines, NFS-60 ( IL-3 ) , MC/9 (MCGF), and HT2 (TCGF). For purification of this factor to homogeneity, 8L of Con-A-activated Cl.l supernatant was dialized into 20mM Hepes buffer, pH 7.0, then passed over a Pharmacia Mono S 10/10 cation exchange column equilibrated with the same buffer. Peak activity eluted with a linear salt gradient at 0.2M NaCl. This material was pooled, concentrated, re-dialized into 20 mM Hepes buffer, pH 7.0, and loaded onto a Heparin-Sepharose column equilibrated with the same buffer. Peak activity eluted at 0.45M NaCl with a linear gradient to 2M NaCl. This material was pooled, diluted lOx with 0.10/TFA/H 2 O, pH 2, and fractionated on a Vydac reverse phase column (c4). Application of a linear gradient of acetonitrile, 0.1% TFA, released the activity at 42% acetonitrile. Example I. de novo Preparation of Murine IL-4 cDNAs from Cl.Lyl + 2 " /9 Cells and Transient Expression in COS 7 Monkey Cells. cDNA clones coding for IL-4 were isolated from the murine helper T cell line Cl.Lyl + 2~/9, which is deposited with the ATCC under accession number CRL 8179 and described by Nabel et al . , in Cell, Vol. 23, pqs. 19- 28 (1981), and in Proc. Natl. Acad. Sci., Vol. 78, pqs. 1157-1161 (1981), Other murine cells known to produce BCGF activity include the EL-4 line, available from the ATCC under accession number TIB 39. The procedures used in this example have been disclosed in Lee et al . , Proc. Natl, Acad. Sci,, Vol. 83, pqs, 2061-2065 (1986). Briefly, a pcD cDNA library was constructed with messenqer RNA (mRNA) from concanavalin A (ConA)-induced Cl.Lyl 2~/9 cells following the procedure of Okayama and Berg, described above. IL-3 and GM-CSF clones were eliminated from a large sublibrary of randomly selected clones by hybridization with 32 P-labeled cDNA probes. Pools and/or individual clones from the remainder of the sublibrary were screened for IL-4 cDNA by transfecting COS 7 monkey cells and testing culture supernatants for MCGF and TCGF activity. A. Induction of IL-4 Production. Cl.Lyl + 2~/9 cells were induced to produce IL-4 mRNA by ConA as follows. The cells are cultured at 5 x 10 5 /ml in Dulbecco's Modified Eagles medium (DME) with 4% heat-inactivated fetal calf serum, 5 x 10 ""5 M 2- mercaptoethanol (2-ME), 2mM glutamine, non-essential amino acids, essential vitamins and 2 ug/ml ConA. After 12-14 hrs' incubation at 37°C in 10% C0 2 , the cell suspension is centrifuged at 1500 rpm for 10 minutes. The cell pellets are collected and frozen immediately at -70°C. B. Isolation of mRNA Total cellular DNA was isolated from cells using the guanidine isothiocyanate procedure of Chirgwin, J. et al. (Biochemistry, 18: 5294-5299 [1979] ). Frozen cell pellets from ConA-induced Cl.Lyl + 2~/9 cells (12 hrs after stimulation) were suspended in guanidine isothiocyanate lysis solution. Twenty ml of lysis Q solution was used for 1.5 x 10° cells. Pellets were resuspended by pipettinq, and then DNA was sheared by 4 passes throuqh a syrinqe usinq a 16 gauqe needle. The lysate was layered on top of 20 ml of 5.7 M CsCl, 10 mM EDTA in 40 ml polyallomer centrifuqe tube. This solution was centrifuged at 25,000 rpm in a Beckman SW28 rotor (Beckman Instruments, Inc., Palo Alto, CA) for 40 hrs at 15°C. The guanidine isothiocyanate phase containing DNA was pipetted off from the top, down to the interface. The walls of the tube and interface were washed with 2-3 ml of guanidine isothiocyanate lysis solution. The tube was cut below the interface with scissors, and the CsCl solution was decanted. RNA pellets were washed twice with cold 70% ethanol. Pellets were then resuspended in 500 ul of 10 mM Tris.HCl pH 7.4, 1 mM EDTA, 0.05% SDS. 50 ul of 3M sodium acetate was added and RNA was precipitated with 1 ml ethanol. About 0.3 mg total RNA was collected by centrifuging and the pellets washed once with cold ethanol. Washed and dried total RNA pellet was resuspended in 900 ul of oligo (dT) elution buffer (10 mM Tris.HCl, pH 7.4, 1 mM EDTA, 0.5% SDS). RNA was heated for 3 min. at 68°C and then chilled on ice. 100 ul of 5 M NaCl was added. The RNA sample was loaded onto a 1.0 ml oligo (dT) cellulose column (Type 3, Collaborative Research, Waltham, MA) equilibrated with binding buffer (10 mM Tris.HCl pH 7.4, 1 mM EDTA, 0.5 M NaCl, 0.5% SDS.) Flow-through from the column was passed over the column twice more. The column was then washed with 20 ml binding buffer. PolyA + mRNA was collected by washing with elution buffer. RNA usually eluted in the first 2 ml of elution buffer. RNA was precipitated with 0.1 volume 3 M sodium acetate (pH -6) and two volumes of ethanol. The RNA pellet was collected by centrifugation, washed twice with cold ethanol, and dried. The pellet was then resuspended in water. Aliquots were diluted, and absorbance at 260 nm was determined. C. Construction of pcD cDNA Library 1) Preparation of Vector Primer and 01igo(dG)-Tailed Linker DNAs. The procedure of Okayama & Berg (Mol. s Cell. Biol. Vol. 2, pgs 161-170 [1982]) was used with only minor modifications. The pcDVl and pLl plasmids are described by Okayama & Berg (Mol. & Cell. Biol. 3: 380- 389 [1983] ) and are available from Pharmacia (Piscataway, N.J.). Specifically, a modified pcDVl plasmid was used which contained an Nsil site at the previous location of the Kpnl site. An 80 ug sample of pcDVl DNA was digested at 30°C with 20 U of Kpnl endonuclease in a reaction mixture of 450 ul containing 6 mM Tris.HCl (pH 7.5), 6 mM MgCl 2 , 6 mM NaCl, 6 mM 2-ME, and 0.1 mq of bovine serum albumin (BSA) per ml. After 16 hr the digestion was terminated with 40 ul of 0.25 M EDTA (pH 8.0) and 20 ul of 10% sodium dodecyl sulfate (SDS); the DNA was recovered after extraction with water-saturated 1:1 phenol-CHC^ (hereafter referred to as henol-CHCl3 ) and then by precipitation with ethanol. Homopolymer tails averaging 60, but not more than 80, deoxythymidylate (dT) residues per end were added to the Nsil endonuclease-qenerated termini with calf thymus terminal transferase as follows: The reaction mixture (38 ul) contained sodium cacodylate-30 mM Tris.HCl pH 6.8 as buffer, with 1 mM CoCl 2 / 0.1 mM dithiothreitol, 0.25 mM dTTP, the Nsil endonuclease-digested DNA, and 68 U of the terminal deoxynucleotidyl transferase (P-L Biochemicals, Inc., Milwaukee, WI ) . After 30 min. at 37°C the reaction was stopped with 20 ul of 0.25 M EDTA (pH 8.0) and 10 ul of 10% SDS, and the DNA was recovered after several extractions with phenol-CHC^ by ethanol precipitation. The DNA was then digested with 15 U of EcoRI endonuclease in 50 ul containinq 10 mM Tris.HCl pH 7.4, 10 mM MqCl 2 , 1 mM dithiothreitol, and 0.1 mg of BSA per ml for 5 hr at 37°C. The large fragment, containing the SV40 polyadenylation site and the pBR322 oriqin of replication and ampiσillin-resistance gene, was purified by aqarose (1%) gel electrophoresis and recovered from the gel by a modification of the qlass powder method (Voqelstein, B. & Gillespie, D. , Proc. Natl. Acad. Sci. 76: 615-619 [1979]). The dT-tailed DNA was further purified by absorption and elution from an oligo (dA)-cellulose column as follows: The DNA was dissolved in 1 ml of 10 mM Tris.HCl pH 7.3 buffer containinq 1 mM EDTA and 1 M NaCl, cooled at 0°C, and applied to an oliqo (dA)- cellulose column (0.6 by 2.5 cm) equilibrated with the same buffer at 0°C and eluted with water at room temperature. The eluted DNA was precipitated with ethanol and dissolved in 10 mM Tris.HCl pH 7.3 with 1 mM EDTA. The oliqo (dG)-tailed linked DNA was prepared by diqestinq 75 ug of pLl DNA with 20 ϋ of PstI endonuclease in 450 ul containinq 6 mM Tris.HCl pH 7.4, 6 mM MgCl 2 , 6 mM 2-ME, 50 mM NaCl, and 0.01 mg of BSA per ml. After 16 hr at 30°C the reaction mixture was extracted with phenol-CHCl- j and the DNA was precipitated with alcohol. Tails of 10 to 15 deoxyguanylate (dG) residues were then added per end with 46 U of terminal deoxynucleotidyl transferase in the same reaction mixture (38 ul) as described above, except that 0.1 mM dGTP replaced dTTP. After 20 min. at 37°C the mixture was extracted with phenol-CHC^, and after the DNA was precipitated with ethanol it was digested with 35 U of Hindlll endonuclease in 50 ul containinq 20 mM Tris.HCl pH 7.4, 7 mM MgCl 2 , 60 mM NaCl, and 0.1 mq of BSA at 37°C for 4 hr. The small oligo (dG)-tailed linker DNA was purified by agarose gel (1.8%) electrophoresis and recovered as described above. 2) cDNA Library Preparation: Step 1: cDNA synthesis. The reaction mixture (10 ul) contained 50 mM Tris.HCl pH 8.3, 8 M MqCl 2 , 30 mM KC1, 0.3 mM dithiothreitol, 2 mM each dATP, dTTP, dGTP, and dCTP, 20 u Ci 32 P-dCTP (3000 Ci/mmole), 3 ug polyA + RNA from Con-A induced T-cells, 60 units RNasin (a tradenamed ribonuclease inhibitor from Promeqa Biotec, Inc., Madison, WI) , and 2 ug of the vector-primer DNA (15 pmol of primer end), and 45 U of reverse transcriptase. The reaction was incubated 60 min at 42°C and then stopped by the addition of 1 ul of 0.25 M ETDA (pH 8.0) and 0.5 ul of 10% SDS; 40 ul of phenol-CHCl 3 was added, and the solution was blended vigorously in a Vortex mixer and then centrifuged. 40 ul of 4 M ammonium acetate and 160 ul of ethanol were added to the aqueous phase, and the solution was chilled with dry ice for 15 min., warmed to room temperature with gentle shaking to dissolve unreacted deoxynucleoside triphosphates that had precipitated during chilling, and centrifuqed for 10 min. in an Eppendorf microfuqe. The pellet was dissolved in 10 ul of 10 mM Tris.HCl pH 7.3 and 1 mM EDTA, mixed with 10 ul of 4 M ammonium acetate, and reprecipitated with 40 ul of ethanol, a procedure which removes more than 99% of unreacted deoxynucleotide triphosphates. The pellet was rinsed with ethanol. Step 2: Oligodeoxycytidylate [oligo (dC)] addition. The pellet containing the plasmid-cDNA:mRNA was dissolved in 20 ul of 140 mM sodium cacodylate-30 mM Tris.HCl pH 6.8 buffer containing 1 mM CoCl 2 , 0.1 mM dithiothreitol, 0.2 ug of poly (A), 70 uM dCTP, 5 uCi 32 P-dCTP, 3000 Ci/mmole, and 60 U of terminal deoxynucleotidyl transferase. The reaction was carried out at 37°C for 5 min. to permit the addition of 10 to 15 residues of dCMP per end and then terminated with 2 μl of 0.25 M EDTA ( H 8.0) and 1 ul of 10% SDS. After extraction with 20 ul of phenol-CHC^, the aqueous phase was mixed with 20 ul of 4 M ammonium acetate, the DNA was precipitated and reprecipitated with 80 ul of ethanol, and the final pellet was rinsed with ethanol. Step 3: Hindlll endonuclease diqestion. The pellet was dissolved in 30 ul of buffer containinq 20 M Tris.HCl pH 7.4, 7 mM MgCl 2 , 60 mM NaCl, and 0.1 mg of BSA per ml and then digested with 10 U of Hindlll endonuclease for 2 hr at 37°C. The reaction was terminated with 3 ul of 0.25 M EDTA (pH 8.0) and 1.5 ul of 10% SDS and, after extraction with phenol-CHCl 3 followed by the addition of 30 ul of 4 M ammonium acetate, the DNA was precipitated with 120 ul of ethanol. The pellet was rinsed with etϊianol and then dissolved in 10 ul of 10 mM Tris.HCl (pH 7.3) and 1 mM EDTA, and 3 ul of ethanol was added to prevent freezing during storage at -20°C. Step 4: Cyclization mediated by the oligo (dG)-tailed linker DNA. A 9 ul sample of the Hindlll endonuclease-digested oligo (dC)-tailed cDNA:mRNA plasmid (about 90% of the sample) was incubated in a mixture (90 ul) containing 10 mM Tris.HCl pH 7.5, 1 mM EDTA, 0.1 M NaCl, and 1.8 pmol of the oligo (dG)-tailed linker DNA at 65°C for 5 min., shifted to 42°C for 60 min, and then cooled to 0°C. The mixture (90 ul) was adjusted to a volume of 900 ul containing 20 mM Tris.HCl pH 7.5, 4 mM MgCl 2 , 10 mM (NH 4 ) 2 S0 4 , 0.1 M KC1, 50 g of BSA per ml, and 0.1 mM 3-NAD; 6 ug of E. coli DNA ligase were added and the solution was then incubated overnight at 12°C. Step 5: Replacement of RNA strand by DNA. To replace the RNA strand of the insert, the ligation mixture was adjusted to contain 40 u of each of the * four deoxynucleoside triphosphates, 0.15 mM R-NAD, 4 ug of additional E. coli' DNA ligase, 16 U of E. coli DNA polymerase I (Poll, ) and 9U of E. coli RNase H. This mixture (960 ul) was incubated successively at 12°C and at room temperature for 1 hr each to promote optimal repair synthesis and nick translation by Poll. Step 6: Transformation of S. coli. Transformation/was carried out using minor modifications of the procedure described by Cohen et al. (Proc. Nat. Acad. Sci. U.S.A. , 69: 2110-2114 [1972] ). S. coli K-12 strain MC1061 (Casadaban, M. and Cohen, S., J. Mol. Biol. 138: 179-207 [1980] ) was grown to 0.5 absorbancy unit at 600 nm at 37°C in 300 ml of L-broth. The cells were collected by centrifugation and suspended in 30 ml of 10 mM Pipes (pH 7), 60 mM CaCl ? , 15% qlycerol, and centrifuged at 0°C for 5 min. The cells were resuspended in 24 ml of the above buffer and incubated again at 0°C for 5 min. ; then 1.0 ml aliquots of the cell suspensions were mixed with 0.1 ml of the DNA solution (step 5) and incubated at 0°C for 20 min. Next the cells were kept at 42°C for 2 min. and thereafter at room temperature for 10 min.; then 1 liter of L-broth was added, and the culture was incubated at 37°C for 60 min. Ampicillin was added to a concentration of 50 ug/ml. The culture was shaken for an additional 10 hrs. at 37°C. Dilutions of this culture were spread on L-broth agar containing 50 ug/ml ampicillin. After incubation at 37°C for 12 to 24 hr, individual colonies were picked with sterile tooth¬ picks. In all, approximately 1 x 10 5 independent cDNA clones were generated. D. Screening the pcD Library. 10 4 single clones were picked at random from the T-cell cDNA library and propagated individually in wells of microtiter dishes containing 200 ul L-broth with ampicillin at 50 ug/ml and dimethyl sulfoxide at 7%. To focus only on the novel MCGF activity, 53 IL-3 cDNA clones and one GM-CSF cDNA clone were identified by hybridization with the appropriate P-labelled cDNA probes constructed from the clones disclosed by Lee et al., Proc. Natl. Acad. Sci., Vol. 82, pgs. 4360-4364 (1985), and by Yokota et al., Proc. Natl. Acad. Sci., Vol. 81, pgs. 1070-1074 (1984), and were eliminated. This procedure was carried out as follows: Each plate of 96 cultures was replicated onto nitrocellulose filters for hybridization screening. Hybridizations were performed in 6XSSPE (1X3SPE = 180 mM NaCl; 10 mM sodium phosphate, pH 7.4; 1 mm EDTA), 0.1% SDS, 100 uq/ l E. coli tRNA, 50% formamide, for 16 hrs. at 42°C. Hybridizing clones were identified by autoradiography of the washed filter. These clones were removed by sterilizinq the microtiter wells containinq these clones with ethanol prior to the preparation of clone pools. Pools containinq up to 48 cDNA clones were prepared from the microtiter cultures. Two hundred such pools were grown up in 1 liter cultures of L-broth containing 100 ug/ml ampicillin. Plasmid DNA was isolated from each culture and purified by twice banding through CsCl gradients. The DNA representing each pool was transfected into C0S7 monkey cells as follows. (COS7 cells are described by Gluzman in Cell, Vol. 23, pgs. 175-180 (1981), and are available from the ATCC under accession number CRL 1651.) One day prior to transfection, approximately 10 COS 7 monkey cells were seeded onto individual 100 mm plates in DME containing 10% fetal calf serum and 2 mM glutamine. To perform the transfection, the medium was aspirated from each plate and replaced with 4 ml of DME containinq 50 mM Tris.HCl pH 7.4, 400 ug/ml DEAE-Dextran and 50 ug of the plasmid DNAs to be tested. The plates were incubated for four hours at 37°C, then the DNA- containing medium was removed, and the plates were washed twice with 5 ml of serum-free DME. DME containinq 150 yM Chloroquine was added back to the plates which were then incubated for an additional 3 hrs at 37°C. The plates were washed once with DME, and then DME containing 4% fetal calf serum, 2 mM glutamine, penicillin and streptomycin was added. The cells were then • incubated for 72 hrs at 37°C. The growth medium was collected and evaluated in the various bioassays. An initial set of plasmid pools was screened primarily by using proliferation assays for TCGF and MCGF activities with the HT-2 cell line (described more fully below) and MC/9 cell line, respectively. Among the first 110 pools assayed on these two cell lines, eight produced significant activity in the HT-2 TCGF assay. Several of these pools had weak but siqnificant MCGF activity, but because the MCGF activities were generally weaker and more variable, we did not rely on this assay for identifying positive pools. Approximately half of the COS supernatants from the random pool transfections were also assayed for la- inducing activity on mouse B cells. Among the pools tested, each pool shown to be active for TCGF activity was found also to have la-inducing activity. Thus, there was a perfect correlation between the TCGF activity and the la-inducing activity. One pool, 2A, which was reproducibly the most active in all assays, was subdivided into 48 smaller sub- pools. Two subpools were positive for both MCGF and TCGF activities. The single clone, 2A-E3, common to both subpools was then grown individually and its plasmid DNA was transfected into COS 7 cells as described above. The resulting COS supernatant was then assayed for the presence of various activities, including MCGF, TCGF, la- inducing, and IgE- and IgG-enhancing activities. A 366 base-pair-long PstI fragment isolated from clone 2A-E3 (Figure 1A) and labelled with 32P was used as a probe to screen pools which had been positive for biological activity as well as other untested pools. The screening was performed by hybridization to filters replicated with the microtiter cultures as described above. Nine hybridizing clones were isolated and their DNA analyzed by restriction mapping. All pools which exhibited biological activity contained at least one hybridizing clone which shared a common restriction cleavage map with clone 2A-E3. The frequency of hybridizing clones among the 10 which were picked suggests a frequency of approximately 0.2% in the total library. Of the hybridizing clones which were tested, approximately 90% expressed a functional protein. E. Biological Activities of Culture Supernatants of COS 7 Monkey Cells Transfected with pcD-2A-E3. Supernatant from COS 7 cells transfected with pcD-2A-E3 was tested for TCGF activity on the murine helper T cell line HT-2, described by Watson in J. Exp. Med. , Vol. 150, pg. 1510 (1979). Proliferation of the HT-2 cells, as determined by the colorimetric assay of Mosmann (cited above) , was used as a measure of TCGF activity (degree of proliferation being correlated to optical density (OD) from 570 to 630 nm) . Figure 3A illustrates the relative TCGF activities at various dilutions of (i) supernatant from COS 7 cells transfeσted with pcD-2A-E3 (curve 1), (ii) supernatant from Cl.Lyl + 2~/9 cultures (curve 2), (iii) supernatant from COS 7 cells transfected with a pcD plasmid carrying IL-2 cDNA (curve 3) , and (iv) supernatant from COS 7 cells transfected with a pcD plasmid containing no cDNA insert (i.e. a "mock" transfection) (curve 4). Similarly, supernatants from pcD-2A-E3 transfected COS 7 cells were tested for MCGF activity on MC/9 cells, again using the colorimetric assay of Mosmann to measure MC/9 proliferation. Figure 3B illustrates relative MCGF activity of (i) supernatant from COS 7 cells transfeσted with pcD-2A-E3 (σurve 1) , (ii) supernatant from COS 7 σells transfeσted with a pσD plasmid σarrying IL-3 σDNA (σurve 2), (iii) supernatant from Cl.Lyl + 2~/9 cells (curve 3), and (iv) supernatant from mock transfected COS 7 cells (σurve 4) . Figure 3C illustrates the results of an la induction assay conducted on (i) supernatant of COS 7 cells transfeσted with pσD-2-E3 (σurve 1), (ii) supernatants of Cl.Lyl + 2"/9 σells (σurve 2), and (iii) supernatants of moσk transfeσted COS 7 σells (σurve 3) . The Ia-induσtion assay was σarried out as desσribed by Roehm et al. (cited above) . Several DBA/2 mice (2-3 months old) were saσrifiσed and the spleens obtained surgically. The erythroσytes were lysed by hypotoniσ shock using 0.87% ammonium chloride. Then the T-cells were lysed by using cytotoxiσ monoσlonal antibodies directed against T-cell-speσifiσ surfaσe markers (Thy-1, Lyt-1 and Lyt-2) followed by incubation in rabbit complement. The dead cells were then removed using ficoll-hypaque density gradients. Adherent σells had been removed previously by adherence to plastic petri dishes at 37°C. At this time the cells were washed, σounted and sσored for viability. Approximately one million σells were inσubated in 0.5 ml of tissue culture medium (RPMI 1640 or Minimal essential medium-MEM/Earle's salts) (Gibco) supplemented with 10% fetal calf serum, 2- mercaptoethanol and various antibiotics (penicillin, streptomycin and gentamiσin) . In experiments where the positive σontrol σonsisted of supernatants from T-σells induced with the T-cell itogen Concanavalin A, 10 mg/ml (final concentration) of alpha-methyl-mannoside was added to neutralize the mitogen. After 24 hours' incubation, the cells were harvested and prepared for staining with anti-I-A or anti-I-A monoclonal antibodies. These antibodies were used as first-stage antibodies conjugated to either the hapten N.I.P. or biotin. The staining was then completed by incubating the cells with fluoresceinated second-stage reagents (either anti-NIP antibodies or avidin) . The intensity of fluorescence staining was then determined using either a fluorescenσe- aσtivated cell sorter (Becton-Diσkinson, Mountain View, CA) or a Cytofluorograph (Ortho Diagnostics, Cambridge, MA) . Fluorescenσe units in Figure 3C are σalσulated by multiplying the perσentage of positive σells in eaσh sample by the intensity of fluorescent staining. Figure 3D graphically illustrates the degrees by which IgE and IgG_ production are induced in T-cell- depleted mouse spleen cells by (i) COS 7 medium alone (bar 1) , (ii) 20% supernatant from oσk transfeσted COS 7 σells (bar 2), (iii) 10% supernatant from Cl.Lyl + 2~/9 σells plus 20% supernatant from oσk transfeσted COS 7 σells (bar 3) , and (iv) 20% supernatant from pcD-2A-Ξ3- transfeσted COS 7 σells (bar 4) . Levels of IgΞ and lgG- j _ were determined by the isotype-specific ELISA described above. Murine IL-4 was found to enhance the MCGF activity of IL-3 in MC/9 σells: Smith and Renniσk, Proc. Natl. Aoad. Sci., Vol. 83, pgs. 1857-1861 (1986). Murine IL-4 was also found to enhance GM-CSF-sti ulated proliferation of the IL-3-dependent cell line, NFS-60, described by Holmes et al., in Proc. Natl. Aσad. Sσi., Vol. 82, pgs. 6687-6691 (1985). F. Structure of pcD-2A-E3 and Nucleotide Sequence of Its cDNA Insert. The structure of pcD-2A-E3 is illustrated diagramatically in Figure 2A, and an expanded restriction map of its insert is illustrated in Figure 2B. The insert was sequenced using both the Maxa and Gilbert approach (Methods in Enzymology, Vol. 65, pgs. 499-560 (1980) ) and the Sanger approach (Proσ. Natl. Aσad. Sσi., Vol. 74, pgs. 5463-5467 (1977)). The sequenσe is illustrated in Figure lA, along with the deduσed amino aσid sequence for the longest open reading frame in-phase with the first ATG start codon. The single long open reading frame in the mouse 2A-Ξ3 cDNA σlone σonsists of 140 amino aσid residues. Beσause this lymphokine is a seσreted protein, a hydrophobiσ leader sequenσe would be expeσted to preσede the sequenσe for the mature seσreted form of the protein. Analysis of the hydrophobiσity of the polypeptide and comparison with a proposed consensus sequence for the processing of signal peptides (Perlman et al., J. Mol. Biol. , Vol. 167, ' pgs. 391-409 (1983)) suggest that cleavage of the preσursor polypeptide would occur following the serine residue at amino acid position 20 in Figure 1A. Grabstein et al. , J. Exp. Med. , Vol. 163, pgs. 1405-1414 (1986), has confirmed that the N- terminal sequence of secreted murine IL-4 begins at the position 21 His of Figure 1A. Example II. Preparation of Human IL-4 Via a Murine cDNA Probe to a Human Helper T Cell cDNA Library and Transient Expression in COS 7 Monkey Cells and Mouse L Cells. cDNA clones coding for IL-4 were isolated from cDNA libraries constructed from an induced human helper T cell, 2F1, and induced human peripheral blood lymphocytes (PBLs) by way of a murine cDNA probe. Other human cell lines known to produce BCGF activity include variants of the CEM line, available from the ATCC under accession numbers CCL 119, CRL 8436, and TIB 195, and described by Foley et al., in Cancer, Vol. 18, pgs. 522-529 (1965), and by Ligler, in Lymphokine Research, Vol. 3, pgs. 183- 191 (1984). A human helper T-cell clone, 2F1, and human PBLs were grown in Iscove's medium supplemented with 3% fetal calf serum. The 2F1 cells were activated with ConA (10 ug/ml) and PBLs were stimulated with 1 ug/ml PNA for 12 hours, after which ConA at 5 ug/ml was added. The cells were harvested 4 hr (2F1) or 10 hr (PBLs) after addition of ConA. mRNA extraction and cDNA library construction were carried out as described in Example I. A PstI fragment was isolated from the mouse pcD-2A-E3 cDNA clone, labeled by nick translation (1 x 10 cpm/ug) and used to probe nitrocellulose filters containing plasmid DNA preparations from ten pools, each representing approximately 1 x 10- 5 clones of 2F1 cDNA library. Low stringency hybridization conditions (overniqht at 42°C) ' were used: βxSSPE (lxSSPE=180 mM NaCl/lOmM sodium phosphate, H 7.4/1 mM EDTA) (Maniatis, T. et al. , Molecular Cloninq: A Laboratory Manual (Cold Spring Harbor Laboratory, N.Y., 1982)), 20% (vol/vol) formamide, 0.1% sodium dodecyl sulfate, yeast carrier tRNA at 100 ul. The filters were washed with 2 x SSPE, 0.1% sodium dodecyl sulfate at 37°C. A single clone (pcD-46) was identified in one of the ten pools. Additional clones were obtained by screeninq the PBL cDNA libraries with a probe constructed from the Nhel-EcoRI fragment of pcD-46 (illustrated in the restriσtion map of Figure 2D). Analysis by restriction enzymes indicated that the PBL clones were identical in structure to pcD-46. It was discovered that a guanidine-rich region in the 5', or upstream, direction from the coding region insert of pcD-46 inhibited expression of the IL-4 polypeptide. Consequently, the insert of pσD-46 was reσloned to remove the guanidine-riσh region. The resulting clone is desiqnated pcD-125. It was also disσovered that expression was improved by transfecting mouse L cells with pcD-46. The vector pcD-125 was formed as follows: pcD-46 was σleaved with Sau3A to isolate a fragment containing the 5' 162 nucleotides of the cDNA insert (elimi ' natinq the GC segment) and then the fragment was inserted into the Bglll site of plOl. The plasmid plOl was derived from pσD-mouse IL-3 (see, Yokota, T. et al., [1984] cited above) and is deleted for the sequence from the PstI site at the 5' end of the cDNA to a Bglll site within the mouse IL-3 cDNA. A Bglll site is included at the junction of the deleted sequence. The Sau3A fragment is fused to the SV40 promoter as in pcD-46, except for the GC seqment. The remainder of the human cDNA was then reconstruσted with a Hindlll/Nhel fraqment from pcD-46 which carries the 3' end of the cDNA, the SV40 poly-A site and all of the pBR322 sequences of pcD-46. Supernatants of the pcD-46- and pcD-125- transfeσted COS 7 and L σells were assayed for BCGF and TCGF aσtivity. TCGF was assayed with a human helper T cell line JL-EBV transformed with Epstein-Barr virus, and phytohemagglutinin-sti ulated human peripheral blood lymphocytes. The human helper T-cell clone JL-EBV was stimulated with irradiated (4500R) cells of a human EBV- transformed 3-σell line, and subsequently maintained in RPMI 1640 medium σontaining .10% human AB serum, 50 . micromolar 2-mercaptoethanol (2ME) and recombinant human IL-2. Human PBLs were stimulated with PHA (20 microgram/ml) and maintained in RPMI 1640 containing 10% fetal calf serum, 50 micromolar 2ME and recombinant human IL-2. Five to ten days after stimulation, JL-EBV cells or PHA blasts were used as targets in a two-day TCGF assay using the Mosmann colorimetric method (described above), or in a three-day TCGF assay using [ H] thymidine incorporation. Figure 4A illustrates the TCGF activities measured by JL-EBV cells (colorimetriσ assay) of (i) supernatant from COS 7 σells transfeσted with pσD plasmids expressing human IL-2 (σurve A) ; (ii) supernatant from L σells transfeσted with pσD-125 (σurve B) ; (iii) supernatant from COS 7 σells transfeσted with pcD-125 (curve C) , (iv) supernatant from COS 7 cells transfected with pcD-46 (curve D) , and (v) supernatant from mock transfected COS 7 cells (curve Ξ) . Figure 4B illustrates the TCGF aσtivities measured by PHA- stimulated PBLs (σolori etriσ assay) of (i) supernatant from COS 7 cells transfected with pcD plasmids expressing human IL-2 (σurve A) , (ii) supernatant from COS 7 σells transfeσted with pσD-125 (curve B) , and (iii) supernatant f om mock transfected COS 7 cells (curve C) . Figure 4C illustrates the TCGF activities measured by PHA- stimulated PBLs (tritiated thymidine incorporation assay) of (i) supernatant from COS 7 cells transfected with pcD- 125 (curve A) , (ii) supernatant from COS 7 cells transfeσted with pcD plasmids expressing human IL-2 (curve B) , and (iii) supernatant from mock transfected COS 7 cells (σurve C) . BCGF aσtivity of various dilutions of pσD-125 transfeσtion supernatants were σompared with the BCGF aσtivity of a BCGF ("commercial BCGF") described by Maizel et al. , Proc. Natl. Acad. Sσi., Vol. 79, pgs. 5998-6002 (1982), and σommerσially available from Cytokine Teσhnology International (Buffalo, NY) . Table II illustrates the BCGF aσtivities of various dilutions of COS 7 transfeσtion supernatants on anti-IgM antibody preaσtivated B σells. B σells were prepared as desσribed in the assay seσtion above. Table II. Effect of the IL-4 cDNA transfection super¬ natants on anti-IgM-preactivated B cells % (vol/vol) H-Thymidine Incorporation (σpm) of Mock- supernatants Mock- Clone transfeσtion Clone 125 added transfection 125 + 10% BCGF + 10% BCGF 0 278 278 1835 1835 0.2 189 144 1362 2303 1 323 1313 1699 3784 5 408 4314 1513 7921 15 397 4289 1093 3487 Table III illustrates the 3CGF aσtivities of various dilutions of COS 7 transfeσtion supernatants on SAC- preaσtivated B σells (prepared as desσribed above) . Table II I Aσti vi ty of the IL-4 CDNA tr ans f eσtion supernatants on SAC- pr eac ti vated B σe ll s % (vol/vol) ^H- ■ Thymidine Incorporation (cpn) of Mock- supernatants Mock- Clone transfeσtion Clcne 125 added transfeσtion 125 + 10% BCGF + 10% BCGF 0 2237 2237 12,992 12, 992 0.2 1789 2682 13,126 5, 655 1 740 2374 13, 714 6, 765 5 1285 2826 5,848 10, 023 15 1560 4701 10,128 10, 924 Although the human IL-4 of the invention and commerσial BCGF both display BCGF aσtivity, Mehta et al. , in J. Immunol. , Vol. 135, pgs. 3298-3302 (1985), demonstrated that TCGF aσtivity can be biochemically separated from the BCGF activity of commerσial BCGF, indiσating that the aσtivities are σaused by separate moleσules. Thus, human IL-4 and σommerσial BCGF are different moleσules beσause TCGF aσtivity is inseparable from BCGF aσtivity in human IL-4, using standard bioσhemiσal fraσtionation teσhniques. Supernatants from COS-7 σells transfeσted with plasmids bearing the human IL-4 cDNA induσe the proliferation of normal human T σells and the human T- σell clone JL-EBV, and activity that is similar to mouse IL-4. However, the maximum extent of proliferation of human T σells induced in response to human IL-4 is about half of that induσed by human IL-2. The proliferation- induσing aσtivity of IL-4 could not be inhibited by monoclonal antibodies against IL-2 or against the IL-2 receptor when tested. These results suggest that IL-4 acts direσtly on T σells and not by way of induσtion of IL-2 and that its aσtivity is not mediated by the IL-2 reσeptor. The COS-human IL-4 supernatants also stimulate the proliferation of human 3 σells preaσtivated with optimal σonσentrations of anti-IgM antibodies σoupled to beads and have additive proliferative σapaσity with σommercial BCGF at saturation levels of the BCGF assay. This suggests that human IL-4 and commerσial BCGF operate on B σells by different routes, e.g. possibly by different sets of receptors. The supernatants did not significantly induce proliferation of B cells preactivated with SAC, whereas σommerσial BCGF purified from supernatants of PBL σultures stimulated with PHA strongly induced the proliferation of SAC-preactivated human B σells. These results further indiσate that the human IL-4 σDNA enσodes a BCGF aσtivity distinσt from that present in the σommerσial BCGF. Supernatant of pσD-125-transfeσted COS 7 σells was also tested for its ability to induce Fc-epsilon receptors on tonsilar B cells. Human tonsil cells were dispersed into a single cell suspension using standard techniques. The B cell population was enriσhed using the protoσol desσribed above, and the enriσhed σells were stimulated with anti-IgM antibody for 24 hours in σulture medium at 37°C. Fσ-epsilon reσeptor-bearing cells were assayed by a 3ecton Diσkinson FACS IV σell sorter using a fluoresσently labeled monoclonal antibody specifiσ for the reσeptor by means of the teσhnique disσlosed by Bonnefoy et al., in J. Immunol. Meth., Vol. 88, pgs. 25- 32 (1986) . Figures 5A-5D are histograms illustrating σell frequenσy (ordinate) versus fluoresσent intensity (absσissa) . Fluoresσenσe intensity is proportional to the number of Fσ-epsilon reσeptors present on a σell. In all the Figures the σells have been stimulated with anti- IgM. Figures 5A through 5D σorrespond to exposures to media σonsisting of 0%, 0.1%, 1%, and 10% supernatant from pσD-125-transfeσted COS 7 σells. The DNA sequenσe of the σDNA insert of σlone no. 46 was determined and is shown in Figure 13. The σDNA insert is 615 bp long, exσluding the pol (A) tail. There is a single open reading frame, with the first ATG σodon located at 64 nucleotides from the 5' end followed by 153 codons ending with the termination codon TAG at nucleotide positions 523-525. The N-terminal segment of the prediσted polypeptide is hydrophobiσ, as would be expeσted for a seσreted protein. A σomparison between the coding regions of a human and a mouse cDNA of the present invention revealed that the regions of the human cDNA coding sequence in pcD-46 covered by amino aσid positions 1-90 and 129-149 share approximately 50% homology with the corresponding regions of the mouse σDNA (2A-E3) coding sequence. These regions, and 5' and 3' untranslated regions, share about 70% homology between the two cDNA sequences from the different species, whereas the region covered by amino acids 91-128 of the human protein shares very limited homology with the corresponding mouse region. In all, six of the seven cysteine residues in the human protein are σonserved in the related mouse protein. Some amino acid sequence homology exists between a native form of a human polypeptide of the present invention and mouse IL- 3. Amino acid residues 7-16 and 120-127 are 50% and 55% homologous, respectively, to residues 16-27 and 41-49 of the mouse IL-3 precursor polypeptide (Yokota, T. et al., Proc. Natl. Aσad. Sci. U.S.A. 81: 1070-1074 [1934]). As desσribed more fully below, human IL-4 purified from pcD-125-transfected COS 7 supernatants was found to be the 129 amino aσid polypeptide having the sequenσe illustrated by Figure IC. Example III. Enhanced Expression of Human IL-4 in COS 7 Monkey cells by Usinq an Epstein-Barr Virus (EBV)-Derived Vector Containinq an RSV-LTR Promoter. A 10-20 fold enhancement of human IL-4 expression was obtained by recloning the Xhol fragment of pcD-125 into an ESV-derived vector containing a Rous sarcoma virus long-terminal repeat (RSV-LTR) promoter. The EBV-derived vector and the RSV-LTR promoter are described by Gorman et al. , Proc. Nat. Acad. Sci., Vol. 79, pgs. 6777-6781 (1982); and Yates et al. , Nature, Vol. 313, pqs. 812-815 (1985). A Hindlll/Xhol fragment containing the RSV-LTR promoter was isolated from a pcD plasmid previously σonstructed from the RSV-LTR containing Acc /Hind ll fragment described by Gorman et al. (cited above) and a commercially available pcD vector (e.g. Pharmacia). The above Hindlll/Xhol fragment and a Hindlll/Xhol fraqment from a pLl plasmid (Pharmacia) containing -an SV40 origin- of- * repliσation (ori) are spliced into plasmid pcDVl (available from Pharmaσia), the orientation of the SV40 ori region not being critical. Between an Aatll site and an Ndel site, the resultinq pcD vector contains in sequence (from the Aatll site) an SV40 ori region, an RSV-LTR promoter, and the SV40 poly A region. After the Xhol fragment of pcD-125 is isolated and inserted into the Xhol site of the newly constructed pcD vector, the unique Aatll and Ndel sites on the vector are converted into Sail sites using standard techniques. Briefly, the pcD vector is diqested with Aatll and Ndel, the IL-4 containing fragment is isolated, and the isolated fraqment is treated with T4 DNA polymerase in the presence of appropriate concentrations of the nucleoside triphosphates. The 5 ' —» 3' DNA polymerase activity of T4 DNA polymerase fills in the 5' protrudinq ends of the restriction cuts, and the 3'—. 5' exonuclease activity of T4 DNA polymerase digests the 3' protruding ends of the restriction cuts to leave a blunt-ended fragment, to which kinased Sail linkers (New Enqland Biolabs) are ligated using T4 DNA ligase. The above Sail fraqment (illustrated in Figure 11) is inserted in the ΞBV-derived vector p201 described by Yates et al . (cited above) at the location of a unique Clal site, which had been converted to a Sail site usinq standard techniques. Briefly, p201 (illustrated in Fiqure 11) is diqested with Clal and treated with DNA polymerase I (Klenow fragment) and appropriate concentrations of nucleoside triphosphates. This procedure fills in the protruding ends of the Clal cut to leave a blunt-ended fragment. Next, the blunt ends are ligated to a kinased Sail linker. The resulting EBV- derived vector containing the RSV-LTR promoter and human IL-4 cDNA insert is referred to herein as pEBV-178. pEBV-178 was transfected into COS 7 cells using standard teσhniques and the culture supernatants were assayed for TCGF activity as a measure of IL-4 expression. Example IV. Expression of Native Human IL-4 and Mutein IS 0 (Ala-Glu-Phe) in E. coli Two vectors containinq human IL-4 cDNA inserts were constructed for expression of human IL-4 in E. coli: a pIN-III secretion vector which contains the signal peptide sequence of the ompA protein ("pIN-III-; ompA2"), and a pUC12 plasmid containinq a trpP promoter and an adjacent ribosome binding site (RBS) region ("TRPC11") . A. pIN-III-ompA2 Two veσtors were σonstruσted using the piN-III- ompA2 plasmid, whiσh is desσribed by Ghrayeb et al., in ΞM30 Journal, Vol. 3, pgs. 2437-2442 (1984), and Masui et al., in Biotechnology, Vol. 2, pgs. 81-85 (1984). The first vector, designated pIN-III-ompA2(1) , was constructed by ligating, in series, the EcoRI/BamHI- digested pIN-III-ompA2 plasmid, a synthetiσ linker, and the BamHI/EcoRV fragment of pcD-125. The synthetic linker used in this σonstruction resulted in the secretion of a biologically aσtive IL-4 polypeptide having the three extra N-terminal amino aσids Ala-Glu- Phe-; i.e. mutein IL (Ala-Glu-Phe) was seσreted. The synthetic linker consisted of the following sequences of nucleotides: AA TC CAC AAG TGC GAT " G GTG TC ' ACG CTA EcoRI/BamHI-digested pIN-III-ompA2 and the BamHI/ΞcoRV fragment of pcD-125 were mixed in a standard ligation solution (e.g. Maniatis et al. , cited above) σontaining 0.1 iσromolar of the synthetiσ linker. E. ooli strain Abl899 was infeσted by the ρIN-III-ompA2(1) plasmid and transformants were selected by colony hybridization using a 32 P-labeled IL-4 cDNA probe. Human IL-4 extracts for assaying were obtained as follows. After sonication, the bacterial cultures were centrifuged, and the supernatant removed from the pellet. The pellet was treated with 1% SDS, 2mM dithiothreitol, and 6M guanidine. The material was reσentrifuged, the supernatant disσarded, and the pellet treated at 45°C with 3% SDS and 2mM dithiothreitol. The material was again σentrifuged, and the supernatant assayed by SDS-PAGE. pIN-III-omp.A(2) was constructed so that the native human IL-4 would be expressed. The three amino acid addition in the ρIN-III-ompA(l) construction was eliminated by site-specifiσ mutagenesis of the ompA signal peptide sequence of pIN-III-ompA2. The site- specific mutagenesis was carried out as disclosed by Zoller and Smith (cited above) . Briefly, the Xbal/Ba HI fragment of pIN-III-ompA2 containing the σoding sequence for the ompA signal peptide (see Fig. 1 in Ghrayeb et al. , cited above) was purified, mixed with purified Xbal/BamHI-digested replicating form (RF) of M13mpl9, ligated, transfected into E. σoli K-12 JM101, and plated, A σlear plaque in the presenσe of IPTG and X-gal was seleσted and propagated, and single stranded DNAS were prepared, e.g. aσσording to the procedures disclosed by Messing in Methods in Enzy ology, Vol. 101 (Aσademiσ Press, New York, 1983). Separately, the following oligonucleotide primer (23-mer) containing the indicated base substitutions (boxed) was synthesized and phosphorylated: 5' - GGAATTCAGAAGCT TG C G GCTAC - 3 This sequence introduσes a seσond Hindlll site in the signal peptide coding region of the mutated pIN-III- ompA2. The oligonucleotide primer was annealed to the M13mpl9 RF containing the Xbal/BamHI fragment of pIN-III- ompA2, and treated with DNA polymerase in the presence of appropriate conσentrations of nucleoside triphosphates. The resulting RFs were used to transfeσt JM101 Ξ. σoli, and utant-σontaining plaques were sσreened by a labeled oligonuσleotide probe. The sequence of the selected RF was confirmed by dideoxy sequenσing using a universal M13 primer. The seleσted RF was propagated, isolated, and digested with Xbal and BamH , and the purified Xbal/BamHI fragment was inserted into an Xbal/BamHI-digested pIN- III-ompA2. To form pIN-III-ompA2(2) , the mutant pIN-III- ompA2 was propaqated, purified, diqested with Hindlll and BamHI, and mixed with the BamHI/EcoRV fragment of pcD-125 in a standard liqation solution containing 0.1 micromolar of the following synthetic linker: A GCT CAC AAG TGC GAT GTG TTC ACG CTA E. coli strain Abl899 was infected by the pIN-III- ompA2(2) plasmid and transformants were selected by -> colony hybridization using a Jώ P-labeled IL-4 cDNA probe. IL-4 extracts, prepared as described above, exhibited TCGF activity comparable to supernatants of pcD-125 COS7 σells. B. TRPC11 The TRPC11 vector was constructed by ligating a synthetic σonsensus RBS fragment to Clal linkers (ATGCAT) and by cloning the resulting fragments into Clal- restricted pMTllhc (which had been previously modified to contain the Clal site). pMTllhc is a small (2.3 kilobase) high copy, AMP R , TET S derivative of pBR322 that bears the EcoRI-HindlH polylinker region of the πVX plasmid (described by Maniatis et al., σited above). It was modified to contain the Clal site by restricting pMTllhc with EcoRI and BamHI, filling in the resultinq sticky ends and ligating with Clal linker (CATCGATG) , thereby restoring the EcoRI and BamHI sites and replacing the Smal site with a Clal site. One transformant from the TRPC11 construction had a tandem RBS sequence flanked by Clal sites. One of the Clal sites and part of the second copy of the RBS sequence were removed by diqestinq this plasmid with PstI, treating with Bal31 nuclease, restricting with EcoRI and treating with T4 DNA polymerase in the presence of all four deoxynucleotide t iphosphates. The resulting 30-40 bp fragments were reσovered via PAGE and σloned into Smal-restriσted pUC12. A 248 bp E. coli trpP- bearing EcoRI fragment derived from pKClOl (described by Niσhols et al. in Methods in Enzymology, Vol. 101, pg. 155 (Aσademiσ Press, N.Y. 1983)) was then cloned into the EcoRI site to complete the TRPCll construσtion, whiσh is illustrated in Figure 12. TRPCll was employed as a veσtor for human IL-4 σDNA by first digesting it with Clal and 3amfll, purifying it, and then mixing it with the EσoRV/BamHI fragment of pσD-125 in a standard ligation solution σontaining 0.1 miσromolar of the following synthetiσ linker: TCG ATG CAC AAG TGC GAT AC GTG TTC ACG CTA The insert-σontaining vector was selected as described above and propagated in E. coli K-12 strain JM101. IL-4 was extraσted as follows. JM101 σells were soniσated in their σulture medium and σentrifugβd. The pellet was resuspended in 4M guanidine and 2mM dithiothreitol, and again σentrifuged. The supernatant was tested for biologiσal activity and found to exhibit TCGF activity comparable to that of supernatants of pcD-125-transfected COS7 cells. Example V. Preparation of Bovine IL-4 cDNAs Via Mouse and Human IL-4 σDNA Probes to a 3ovine Helper T Cell σDNA Library and Transient Expression in COS 7 Monkey Cells. cDNA σlones coding for IL-4 are isolated from cDNA libraries constructed from induced bovine peripheral blood lymphocytes (PBLs) by way of combined mouse and human cDNA probes. Alternative sources of bovine cDNAs include several bovine cell lines maintained in the ATCC's NBL animal line collection. Procedures are substantially identical to those described in Example II. Cells are harvested about 10 hours after induction by ConA. mRNA extraσtion and cDNA library construσtion are σarried out as in Example II. The mouse and human cDNA probes can be used together as a mixture or sequentially to detect bovine IL-4 cDNAs. As in Example II, the PstI fragment is isolated from the mouse pcD-2A-Ξ3 σDNA σlone. Likewise the PstI fragment is isolated from the human pcD-125 cDNA σlone. Several other fragments are also available to construct probes from. Either together or separately the isolated PstI fragments are labeled by nick translation (about 1 x 10° cpm/microgram) and are used to probe nitrocellulose filters containing plasmid DNA preparations from 10 pools, each representing about 1000 clones of the induced PBL cDNA library. Filter hybridization is carried out as in Example II. Positive scoring clones are identified and propagated. Example VI. Expression of Native Human IL-4 and Muteins A 1"4 and IS 0 (Gly-Asn-Phe-Val-His- Gly) in Saccharomyces cerevisiae Native human IL-4 cDNA and two mutants thereof were cloned into the plasmid pMF-alpha8 and expressed in the yeast Saccharomyces cerevisiae. The construction and application of pMF-alpha8 for expressing non-yeast proteins is described fully in Miyajima et al., Gene, Vol. 37, pgs. 155-161 (1985); and Miyajima et al . , SMBO Journal, Vol. 5, pgs. 1193-1197 (1936). pMF-alpha3 is deposited with the American Type Culture Collection (Rockville, MD) under acσession number 40140, and a map of the plasmid is given in Figure 13A (desiqnations in the figure are defined fully in Miyajima et al . , Gene, ci ed above) . A. Human IL-4 Mutein Δ 1"4 . Plasmid pcD-125 was isolated and digested with ΞcoRV and BamHI. The EcoRV/BamHI fraqment containing the human IL-4 cDNA was isolated, treated with DNA polymerase I (Klenow fragment) to fill in the 3amHI cut, and kinased (i.e. phosphorylated) . pMF-alpha8 was digested with StuI and combined with the kinased EcoRV/BamHI fragment of pcD-125 in a standard ligation solution to form plasmid phIL-4-2. phIL-4-2 was used to transform S. cerevisiae 20B-12 (MATalpha trpl-289 pep4-3) , which was obtained from the Yeast Genetic Stock Center, University of California, Berkeley. Yeast cells were grown in synthetic medium containing 0.67% Yeast Nitrogen Base without amino acids, 2% glucose, and 0.5% Casamino acids (Difco). The yeast cells were transformed with the plasmids by the lithium acetate method of Ito et al., J. Bacteriol., Vol. 153, pgs. 163-168 (1983), and transformants were selected, in synthetic medium laσking tryptophan. Supernatant of a transformant culture was tested for TCGF activity. Figure 13B (curve D) illustrates the TCGF activity of several dilutions of the supernatant from phIL-4-2-transformed yeast cells in comparison with other factors (Curve A — human IL-2; Curve B — supernatant from pcD-125-transfected COS 7 cells; and Curve C — supernatants from phIL-4-1- transformed yeast cells). Curve E illustrates the TCGF activity of supernatant from yeast that had been transformed with pMF-alpha8 lacking the IL-4 cDNA insert, i.e. the "mock" transformant. B. Human IL-4 Mutein IS 0 (Gly-Asn-Phe-Val-His-Gly) . The pMF-alpha8 insert for expression of mutein IL°(Gly-Asn-Phe-Val-His-Gly) was prepared exactly as for mutein A 1 , except that the Nael/BamHI fragment from pcD-125 was used. The resulting plasmid was designated phIL-4-1. Several dilutions of supernatant from phIL-4- 1-transformed yeast cells were tested for TCGF activity. The results are illustrated by Curve C of Figure 13B. The supernatants were also tested for BCGF activity on both anti-IgM and SAC-activated B lymphoσytes. The assays were performed as described above, and the results are given in Table IV. Table IV. BCGF Activity of Supernatants of phIL-4-3 Transformed Yeast Cells % (vol/vol) [ H]-Thymidine Incorporation (cpm) of supernatants SAC-activated Anti-IgM Bead Activated added B Lymphocytes B Lymphocytes .0 3633 ± 1239 641 ± 69 0.09 7610 ± 310 13221 ± 472 0.19 9235 ± 181 0.39 10639 ± 786 16681 ± 310 0.78 10372 ± 572 18090 ± 1248 1.56 9905 ± 328 17631 ± 1216 3.12 11354 ± 836 18766 ± 1179 6.25 10481 ± 541 19810 ± 1349 12.5 9641 ± 30 18136 ± 1126 25. 8253 ± 857 14750 ± 1125 C. Expression of Native Human IL-4 in Yeast. cDNA coding for native human IL-4 was cloned into pMF-alpha8 by first inserting bases upstream of the N-terminal His codon to form a Kpnl restriction site. After cleavage by Kpnl and treatment by DNA polymeras I, the blunt-ended IL-4 cDNA was inserted into the StuI site of pMF-alpha8. The Kpnl site was formed by use of standard site-specific mutaqenesis. Briefly, pcD-125 was digested with 3amHI, and the fraqment containinq the entire human IL-4 cDNA was isolated and inserted into the BamHI site of M13mp8. Single-stranded M13mp8 containing the insert was isolated and hybridized to the following synthetic oligonucleotide which served as a primer: 5* - TCCACGGA GGTAC CACAAGTG - 3 The inserted nucleotides are boxed. The plasmid containing the mutated IL-4 cDNA was identified by an oligonucleotide probe, propagated, isolated, and treated with Kpnl and BamHI. The KpnI/BamHI fragment was isolated, treated with DNA polymerase I (Klenow fragment) to generate blunt ends, kinased, and ligated with StuI- digested pMF-alpha8. Yeast was transformed by the resulting pMF-alpha8 plasmids, designated phIL-4-3, as described above, and supernatants were tested for TCGF activity. The supernatants displayed TCGF activity comparable to that observed for supernatants of ρhIL-4-1- transformed yeast. Example VII. Construction and Expression of a Synthetic Human IL-4 Gene in E. coli A synthetic human IL-4 gene is construσted whiσh substantially σomprises baσterial-preferred σodons, inσludes a series of unique restriσtion endonuclease sites (referred to herein as "unique restriσtion sites"), and permits rapid and σonvenient expression of a wide variety of human IL-4 muteins. The nuσleotide sequence of the synthetic gene is illustrated in Figure 6A. Teσhniques for σonstruσting and expressing the synthetiσ gene of this example are standard in the art of moleσular biology, e.g. espeσially Sproat and Gait, uσleic Acids Research, Vol. 13, pgs. 2959-2977 (1985); and Ferretti et al. , Proc. Natl. Acad. Sci., Vol. 83, pgs. 599-603 (1986); but also Mullenbach et al. , J. Biol. Chem. , Vol. 261, pgs. 719-722 (1986); Wells et al. , Gene, Vol. 34, pgs. 315-323 (1985); and Estell et al. , Sσienσe, Vol. 233, pgs. 659-663 (1986). Briefly, the synthetiσ human IL-4 gene is assembled from a plurality of σhe iσally synthesized double-stranded DNA fragments. Base sequenσes of the synthetiσ gene are seleσted so that the assembled synthetiσ gene contains a series of unique restriσtion sites. The series of unique restriσtion sites defines a series of segments whiσh σan be readily exσised and replaσed with segments having altered base sequences. The synthetic fragments are inserted either directly or after ligation with other fragments into a suitable vector, such as a pϋC plasmid, or the like. The above- mentioned segments roughly correspond to the "cassettes" of Wells et al. (cited above) . The synthetic fragments are synthesized using standard techniques, e.g. Gait, Oligonucleotide Synthesis: A Practical Approach (IRL Press, Oxford, UK, 1984). Preferably an automated synthesizer is employed, such as an Applied 3iosystems, Inc. (Foster City, CA) model 380A. pUC plasmids and like vectors are commercially available, e.g. Pharmacia-PL, or Boehringer-Mannheim. Cloning and expression can be carried out in standard baσterial systems, for example E. σoli K-12 strain JM101, JM103, or the like, desσribed by Viera and Messing, in Gene, Vol. 19, pgs. 259-268 (1982). Restriσtion endonuclease digestions and ligase reactions are performed using standard protocols, e.g. Maniatis et al. , Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, New York, 1982). The alkaline method (Maniatis et al., cited above) is used for small-scale plasmid preparations. For large-sσale preparations a modifiσation of the alkaline method is used in whiσh an equal volume of isopropanol is used to preσipitate nuσleiσ acids from the cleared lysate. Precipitation with cold 2.5 M ammonium acetate is used to remove RNA prior to cesium chloride equilibrium density centrifugation and detection with ethidium bromide. For filter hybridizations Whatman 540 filter circles are used to lift colonies which are then lysed and fixed by successive treatments with 0.5M NaOH, 1.5M NaCl; 1M Tris.HCl pH8.0, 1.5M NaCl (2 min each); and heatinq at 80°C (30 min). Hybridizations are in δxSSPE, 20% formamide, 0.1% sodium dodecylsulphate (SDS), 100 uq/ml E. coli tRNA, 100 ug/ml Coomassie Brilliant Blue G- 250 (Biorad) at 42°C for 6 hrs using 32 P-labelled (kinased) synthetic DNAs. (20xSSPE is prepared by dissolving 174 g of NaCl, 27.6 q of NaH 2 P0 4 *H 2 0, and 7.4 q of EDTA in 800 ml of H 2 0, adjusting pH to 7.4 with NaOH, adjusting volume to 1 liter, and sterilizing by autoclaving. ) Filters are washed twice (15 min, room temperature) with lxSSPE, 0.1% SDS. After autoradiography (Fuji RX film), positive colonies are located by aligninq the reqrown colonies with the blue- stained colonies on the filters. DNA is sequenced by either the chemical deqradation method of Maxam and Gilbert, Methods in Enzymoloqy, Vol. 65, pq. 499 (1980), or the dideoxy method of Sanger et al. Proc. Natl. Acad. Sci., Vol. 74, pg. 5463 (1977). Templates for the dideoxy reactions are either single-stranded DNAs of relevant regions recloned into M13mp vectors, e.g. Messing et al. Nucleic Acids Res. , Vol. 9, pg. 309 (1981), or double-stranded DNA prepared by the minialkaline method and denatured with 0.2M NaOH (5 min, room temperature) and precipitated from .2M NaOH, 1.43M ammonium acetate by the addition of 2 volumes of ethanol. Dideoxy reactions are done at 42°C. DNA is synthesized by phosphora idi e chemistry usinq Applied Biosyste s 380A synthesizers. Synthesis, deprotection, cleavace and purification (7M urea PAGE, elution, DEAE-cellulose chromatography) are done as described in the 380A synthesizer manual. Complementary strands of synthetic DNAs to be cloned (400ng each) are mixed and phosphorylated with polynucleotide kinase in a reaction volume of 50 yl. This DNA is ligated with 1 u of vector DNA diqested with appropriate restriction enzymes, and liqations are done in a volume of 50 ul at room temperature for 4 to 12 hours. Conditions for phosphorylation, restriction enzyme digestions, polymerase reactions, and ligation have been desσribed (Maniatis et al., cited above). Colonies are scored for lacZ "1" (when desired) by plating on L agar supplemented with ampicillin, isopropyl-1-thio-beta-D-galactoside (IPTG) (0.4 M) and 5-bromo-4-chloro-3-indolyl-beta-D- galaσtopyranoside (x-gal) (40 ug/ml). The TAC-RBS vector was constructed by a sequence of steps, starting with filling-in with DNA polymerase the single BamHI site of the tacP-bearing plasmid pDR540 (Pharmacia). The product was then ligated to unphosphorylated synthetic oliqonucleotides (Pharmaσia) which form a double-stranded fragment encodinq a consensus ribosome binding site (RBS, GTAAGGAGGTTTAAC) . After ligation, the mixture was phosphorylated and religated with the SstI linker ATGAGCTCAT. The resulting complex was then cleaved ' with SstI and EcoRI, and the 173 bp fragment isolated via polyacrylamide gel electrophoresis (PAGE) and cloned into ΞcoRI/Sstl-restrieted pUC18 (Pharmaσia) (as described below). The sequence of the RBS-ATG-polylinker reqions of the final construction (called TAC-RBS) is shown in Figure 3. The synthetic human IL-4 gene is assembled into a pUC18 plasmid in six steps. At each step inserts free of deletions and/or inserts can be detected after cloning by maintaininq the lacZ ( a) qene of pUCl3 in frame with the ATG start codon inserted in step 1. Clones -37- containing deletion and/or insertion changes can be filtered out by scoring for blue colonies on L-ampicillin plates σontaining x-gal and IPTG. Alternatively, at each step sequences of inserts can be readily confirmed using a universal sequencing primer on small-scale plasmid DNA preparations, e.g. available from Boehringer Mannheim. In step 1 the TAC-RBS vector is digested with SstI, treated with T4 DNA polymerase (whose 3' exonuclease activity digests the 3' protruding strands of the SstI cuts to form blunt-end fragments) , and after deactivation of T4 DNA polymerase, treated with EcoRI to form a 173 base pair (bp) fragment containing the TAC-RBS region and having a blunt end at the ATG start codon and the EcoRI cut at the opposite end. Finally, the 173 bp TAC-RBS fragment is isolated. In step 2 the isolated TAC-RBS fragment of step 1 is mixed with EcoRI/Sstl-digested plasmid pUClδ and synthetic fragment lA/B, which as shown in Figure 7A has a blunt end at its upstream terminus and a staggered (sticky) end corresponding to an SstI out at its downstream terminus. The fragments are ligated to form the ρUC18 of step 2. In step 3 synthetiσ fragments 2A/B and 3A/B (illustrated in Figure 7B and 7C) are mixed with SstI/3amHI-digested ρUC18 of step 2 (after amplification and purification) and ligated to form pUClδ of step 3. Note that the downstream terminus of fragment 2A/B σontains extra bases whiσh form the BamHI staggered end. These extra bases are σleaved in step 4. Also fragments 2A/B and 3A/B have σomplementary 9-residue single-stranded ends whiσh anneal upon admixture, leaving the upstream SstI out of 2A/3 and the downstream BamHI out of 3A/3 to ligate to the pUClS. In step 4 MluI/Xbal-digested pUC18 of step 3 (after amplifiσation and purifiσation) is re urified, mixed with synthetic fragment 4A/B (Figure 7D) , and ligated to form pUC13 of step 4. In step 5 Xbal/Sall-digested pUC18 of step 4 (after amplification and purification) is mixed with synthetiσ fragment of 5A/B (Figure 7E) and ligated to form the pUC18 of step 5. In step 6 SalI/HindIII-diges ed pUC18 of step 5 (after amplification and purification) is mixed with synthetic fragment 6A/B (Figure 7F) and ligated to form the final σonstruσtion. Figure 6B is a σleavage map of the unique restriσtion sites present in the ρUC18 σonstruσtion just desσribed. When the disσlosed synthetiσ human IL-4 gene is used as an insert of pUC18, each pair of unique restriσtion sites defines a segment whiσh can be readily excised and replaced with altered synthetic segments. The set of unique restriσtion sites includes EcoRI, Hpal, SacI (SstI) , EcoRV, PstI, Mlul, Bell, Xbal, Nae Sail, Xhol, and Hindlll. The pUC18 containing the synthetic IL-4 gene is inserted in E. coli K-12 strain JM101. After culturing, protein is extracted from the JM101 cells and dilutions of the extracts are tested for biological activity. Example VIII. Construσtion and Expression of Human IL-4 CO Mutein lie-** in Ξ. σoli. Leu at position 52 (relative to the N-ter inus of the native human IL-4) is changed to He to form human IL-4 mutein He 52 . The ρUC18 plasmid of Example VII containing the synthetic human IL-4 gene of Figure 6A is digested with PstI and Mlul and purified. The above purified pϋC18 is mixed with the synthetic double- stranded fragment illustrated below and ligated. The altered part of the base sequence is boxed. The resulting pUClδ is transfected into Ξ. coli K-12 strain JMlOl, or the like, and expressed. GA GCT GCT ACC GTT ATC CGT ACG TCT CGA CGA TGG CAA TAG GCA CAG TTC TAC TCT CAC CAC GAA AAA GTC AAG ATG AGA GTG GTG CTT TTT GAC A CTG TGC GC Pstl/Mlul Replacement Fragment For Generating Human IL-4 Mutein lie 2 After culturing, protein is extracted from the JMlOl cells using standard techniques, and dilutions of the extracts are tested for biological activity. Example IX. Construction and Expression of Human IL-4 Mutein (He 52 , ASP 111 ) The modified pUC18 plasmid of Example VIII (containing the lie coding sequence) is digested with Sail and Xhol, and the large fragment is isolated. The isolated fragment is mixed with the synthetiσ double- stranded fragment illustrated below in a standard ligation solution. The altered part of the sequenσe is boxed. The resulting plasmid is transfected into Ξ. coli K-12 strain JMlOl, or the like, and expressed. TCG ACT CTG GAA GAC TTC C GA GAC CTT CTG AAG GAG CT Sall/Xhol Replacement Fraqment After culturinq, protein is extracted from the JMlOl cells using standard techniques, and dilutions of the extracts are tested for biological activity. Example X. Sequence of Human IL-4 Purified from Transfection Supernatants Human IL-4 was purified from culture supernatants of cells transiently transfected with veσtors containing human IL-4 cDNA. The sequence of the secreted native human IL-4 was determined from the purified material. A. Biological Assay for Purification. TCGF activity was used to assay, human IL-4 during the separation procedures. The assay was substantially the same as that described in Example II. Briefly, blood from a healthy donor was drawn into a heparinized tube and layered onto Ficoll-Hypaque; e.g., 5 ml of blood per 3 ml Ficoll-Hypaque in a 15 ml centrifuqe tube. After centrifuqation at 3000 x g for 20 minutes, cells at the interface were aspirated and diluted in a growth medium consistinq of RPMI 1640 containinq 10% fetal calf serum, 50 micromolar 2-mercaptoethanol , 20 microqram/ml phytohemaqqlutinin (PHA), and recombinant human IL-2. After 5-10 days of incubation at 37°C, the PHA-stimulated peripheral blood lymphocytes (PRLs) were washed and used in 2-day colorimetric assays: Mossmann, J. Immunol. Methods, Vol. 65, pqs. 55-63 (1983). Serial two-fold dilutions of the IL-4 standard (supernatants from either pcD-125- or pEBT-178-transfected COS 7 cells) or the fraction to be tested were performed in 96-well trays utilizinq the qrowth medium described above to yield a final volume of 50 microliters/well . 50 microliters of the PHA-stimulated PBLs at about 4-8 x 10 6 celIs/ml were added to each well and the trays were incubated at 37°C for 2 days. Cell growth was then measured acσording to Mosmann (σited above) . Units of human IL-4 TCGF activity are defined with respeσt to supernatants of either pσD-125- transfeσted COS 7 cells (Example II) or pEBV-178- transfeσted COS 7 σells (Example III) . For purifiσation, units are based on the aσtivity of pσD-125-transfeσtion supernatants, whiσh are " produσed as follows. About 1 x 10 COS 7 σells are seeded onto 100 mm tissue σulture plates σontaining Dulbecco's Modified Eagle's medium (DME), 10% fetal calf serum, and 4 mM L-glutamine. About 24 hours after seeding, the medium is aspirated from the plates and the σells are washed twice with serum-free buffered (50 mM Tris) DME. To each plate is added 4 ml serum-free buffered DME (with 4 mM L-glutamine) , 80 microliters DEAE-dextran, and 5 micrograms of pcD-125 DNA. The σells are incubated in this mixture for 4 hours at 30°C, after whiσh the mixture is aspirated off and the σells are washed once with serum-free buffered DME. After washing, 5 ml of DME with 4 mM L-glutamine, 100 micromolar Chloroquine, and 2% fetal calf serum is added to eaσh plate, and the cells are incubated for 3 hours and then twice washed with serum-free buffered DME. Next, 5 ml DME with 4 mM L-glutamine and 4% fetal σalf serum is added and the σells are incubated at 37°C for 24 hours. Afterwards the cells are washed 1-3 times with DME or PBS, 5 ml serurn-free DME (with 4 mM L-glutamine) is added, and the σells are inσubated at 37°C until σulture supernatants are harvested 5 days later. One unit, as used herein, is the amount of factor which in one well (0.1 ml) stimulates 50% maximal proliferation of 2 x 10 4 PHA-stimulated PBLs over a 43 hour period. B. Purification Purification was accomplished by a sequential application of cation-exchange chromatography, gel filtration and reverse-phase high-pressure liquid chromatography. All operations were performed at 4°C. After removal of the COS 7 cells by centrifugation, the supernatant was concentrated about 10 fold by ultrafiltration and stored at -80°C until further processed. IL-4 titers were determined by assayinq for the ability of the protein to stimulate proliferation of phytohemagqlutinin-induced human peripheral blood lymphoσytes, i.e. by TCGF activity using the standard assay described above. Concentrated COS 7 supernatant, having TCGF activity of about 10 -10 units/ml and a protein content of about 15-20 mg/ml, is dialyzed against 2 changes of 50 mM sodium HEPES, pH 7.0 over a 24 hour period (each change being approximately 10-15 times the volume of one conσentrate) . The dialysate was applied to a column (1 x 2.5 cm) of S-Sepharose (flow rate: 0.2 ml/min) pre- equilibrated with 50 mM sodium HEPES, pH 7.0. T+ie column was washed with 15 column volumes of equilibrating buffer followed by elution with 20 column volumes of a linear sodium chloride gradient extending from 0 to 0.5 M sodium chloride in 50 mM sodium HEPES, pH 7.0. The gradient was terminated by elution with 5 column volumes of uniform concentration: 50 mM sodium HEPES, 0.5 M NaCl, pH 7.0. 1.5 ml and 1.8 ml fractions were collected from respective batcljjes. IL-4 titers were found for both chromatoqraphies to elute between 300 mM and 500 mM sodium chloride. Six fractions from each S-Sepharose column containing IL-4 titers were separately combined for total volumes of 9.0 and 10.8 ml. Both volumes were concentrated to 1.9 ml by ultrafiltration usinq an Amicon YM5 membrane (molecular weight cut-off: 5000). The recovery of protein from this step was about 30%. The concentrated IL-4 solution was applied to a Sephadex G- 100 column (1.1 x 58 cm) pre-equilibrated in 50 mM HEPES, 0.4 M NaCl, pH 7.0, and the column was eluted with the same buffer at 0.15 ml/min. A total of 50 fractions (1.0 ml/fraction) were collected and analyzed for IL-4 titers. A peak in biological activity was observed at an apparent molecular weiqht of 22,000 daltons. The Sephadex G-100 was calibrated for apparent molecular determination with bovine serum albumin (65,000 daltons), carbonic anhydrase (30,000 daltons) and cytochrome C (11,700 daltons). A fraction from the Sephadex G-100 column containing IL-4 activity was concentrated 3-4 fold in vacuo and was injected onto a Vydac C-4 guard column (4.6 x 20 mm). A linear gradient of 0 to 72% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid (TFA) was produced in 15 minutes at a column temperature of 35° and a flow rate of 1.0 ml/min. Three peaks resulted that were detected at 214 nm with retention times of 7, 8.2 and 8.7 min. (peaks 1, 2, and 3 of Figure 10, respectively). A 40 microliter aliquot of peak 2 (8.2 min. elution time) was lyophilized and redissolved in minimal essential medium containing 10% fetal calf serum. This solution showed a positive TCGF response. A 300 microliter aliquot of peak 2 was evaporated to dryness and redissolved in 200 ul of 0.1% (w/v) sodium dodecyl sulfate (SDS). A 2 ul aliquot was diluted in 200 ul of 1% (v/v) TFA and rechromatoqraphed. The HPLC of this sample demonstrated a single peak at 215 nm. Peak 2 q material indicated an activity of about 7 x 10 Q units/mg. C. Amino Acid Sequence Analysis Amino Acid sequence determination was performed by automated qas-phase Edman degradation (He ick, R. . , Hunkapillar, M.W. , Hood, L.E. and Dryer, W.J. (1981) J. Biol. Chem. 256: 7990) employinq an Applied Biosystems microsequenator. A 90 microliter aliquot of the peak 2 HPLC fraction, dissolved in 0.1% SDS as described above, was applied to the qlass fiber filter cartridge in the presence of Polybrene. Amino aσid sequenσe information was obtained up to the 35th residue. The N-terminal sequenσe was determined as follows His - Lys - - Asp - He - Thr - Leu - Gin - Glu - He - He - Lys - Thr - Leu - Asn - Ser - Leu - Thr - Glu - Gin - Lys - Thr - Leu - - Thr - Glu - Leu - - Val - Thr - Asp - He - Phe - Ala - Ala wherein the blanks indicate the lack of an identifiable amino acid. Blanks in the the amino-terminus at positions 3 and 23 were consistent with the presence of cysteine, which cannot be detected in this system. The blank at position 28, corresponding to a threonine in the cDNA- predicted sequence, may have been due either to the variability of phenylthiohydantoin-threonine detection or to the presence of 0-linked glycosylation or esterification. A 100 microliter aliquot of the HPLC fraction on which the amino-terminal seguence was performed was evaporated to dryness and redissolved in 70% formic acid. A 50-fold molar excess of cyanogen bromide was added and the solution was allowed to stand at room temperature for 2.5 hours. The cleaved protein was sequenced on the Applied Biosystems gas-phase sequenator, as described above. Two sequences were identifiable: (1) Arg - Glu - Lys - Tyr - Ser - Lys (2) His - Lys - - Asp - He - Thr Sequence (1) is identical to the sequence predicted from the cDNA to have been released following cyanogen bromide cleavage of the methionine residue at position 120. The last two residues of the C-terminus may have been present but may not have been detectable owing to insufficient sample. Sequence (2) is identical to the amino-terminal sequence obtained for the native IL-4 protein, as described above. The relative amounts of amino-terminal and carboxyl-terminal phenylthiohydantoin-amino acids released suqqested equimolar amounts of both sequences in the sample. This result supports the conclusion that the protein sample that was sequenced contained predominantly a single polypeptide chain with amino and carboxyl termini predicted from the cDNA sequence of human IL-4. Example XI. Construction and Expression of Human IL-4 Mutein (He 52 , A 71 , IS 94 (Ala)) The modified pUC18 plasmid of Example VIII (containing the lie 52 codinq sequence) is diqested with Mlul and Bell, and the large fragment is isolated. The isolated fragment is mixed with the synthetic double- stranded fragment illustrated below in a standard liqation solution. The resulting plasmid is transfected into E. coli K-12 JMlOl, or the like, and propaqated. CG CGT TGT CTC GGC GCC ACT A ACA GAG CCG CGG TGA GCG CAG TTC CAC CGT CAC AAA GAG CT CGC A GTC AAG GTG GCA GTG TTT GTC GAC TAG Deletion Mlul/Bcll Replacement Fragment The modified plasmid is isolated and digested with Xbal and Nael, and the large fragment is isolated. The isolated UD C fragment is mixed with the synthetic double- J C CD stranded fragm CDJ C ent illustrated below in a standard ligation solution. The added σodon is boxed. The resulting plasmid is transfeσted into Ξ. σoli K-12 strain JMlOl, or the like, and expressed. CTA GAC CGT AAC CTG TGG GGC TG GCA TTG GAC ACC CCG CTG GCC GAC CGG • Xb<al/Nael Replaσe ent Fragment After culturing, protein is extracted from the JMlOl cells using standard techniques, and dilutions of the extraσts are tested for biologiσal aσtivity. Example XII. Induσtion of PR Antigens on Cells from a Patient Suffering from Bare Lymphoσyte Syndrome. Bare lymphoσyte syndrome is σharaσterized by the laσk of expression of σlass I and/or σlass II HLA antigens on σell surfaces, and is frequently associated with severe i munodeficienσy, e.g. Touraine, Lanσet, pgs. 319-321 (February 7, 1981) ; Touraine and Bethel, Human Immunology, Vol. 2, pgs. 147-153 .(1981) ; and Sullivan et al., J. Clin. Invest. , Vol. 76, pgs. 75-79 (1985). It was disσovered that human IL-4 was capable of inducing the expression of the σlass II DR antigen on the surfaσes of cells derived from a patient sufferinq from bare lymphocyte syndrome. Peripheral blood lymphocytes (PBLs) were obtained from a patient sufferinq from non-expression of HLA class II antiqens. 8 cells were purified from the PBLs essentially as described above, and a B cell line (desiqnated UD31) was established by transformation with Epstein-Barr virus (EBV). The EBV-transformed cells were cultured for 48 hours in Yssel's defined medium (described above) with 2% fetal σalf serum and a 5% (v/v) concentration of supernatant from pcD-125-transfected COS 7 cells. The cells were harvested, fixed, stained with fluorescently-labeled anti-DR monoclonal antibody (e.g. Becton Dickinson, L243), and analyzed by flow cytometry. Figure 9 illustrates histograms of cell frequency versus fluorescence intensity for a control population of the EBV-transformed cells harvested prior to IL-4 treatment (Curve A), and for the population of EBV-transformed cells after IL-4 treatment (Curve B). Example XIII. MCGF Activity of Cl.Lyl + 2 " /9 (Cl.l) Supernatants. Purified IL-3 and supernatant from COS 7 cells transfected with an IL-3 cDNA σlone (recombinant IL-3; IL-3 R ) stimulated proliferation of the mast cell line MC/9 to the same extent (Fig. 14A). However, this level was a third to a half of that induced by Cl.l T cell supernatant. The presence of ConA in the T cell super¬ natant (2 ug/ml) does not contribute significantly to its MCGC activity. One explanation for the effect noted above is that IL-3 does not account for all of the MCGF activity produced by Cl.l cells. Alternatively, the mast cell line used for assays could (theoretically) be contaminated with a second factor-dependent population of cells. This latter possibility is excluded, however, because re-σloning the MC/9 cell line yielded subclones, each of which responds to IL-3 and the cell supernatant in the same way as the original MC/9 clone. Since Cl.l supernatant also contains lympho¬ kines other than IL-3, these were tested for their ability to promote the growth of MC/9 cells. However, MC/S cells were unresponsive to various conσentrations of reσombinant IL-2, IFN and GM-CSF, as well as to supernatant σontaining IL-1 (P388D 1 cells) (Fig. 14A) . Moreover, supplementation of cultures σontaining varying concentrations of recombinant IL-3 with IL-2, GM-CSF, IFN-γ, or IL-1 did not stimulate proliferation of MC/9 cells above the level obtained with IL-3 alone. This indicates that the increased MCGF aσtivity of the T cell supernatant is not due to its content of these other known factors. Comparative Studies with Other Faσtor-Dependent Cell Lines. Two other mast σell lines, DX-2 and MM3, also gave higher proliferative responses with Cl.l supernatant than with IL-3 (representative data for DX-2 shown in Figure 14B). These cells also did not respond to IL-2, IFN-γ, GM-CSF or supernatant of P388D-1 cells. Thus, the enhanced proliferative response of MC/9 cells to the T- cell supernatant may be typical of mast cells in general. In contrast, granulocytic-type cells (NFS-60) were stimulated almost equally by IL-3 and Cl.l super¬ natant (Fiq. 14C). Although NFS-60 cells were unresponsive to IL-2, IFN-γ and P388D-1 supernatant, there was a small but reproducible stimulation by GM- CSF. The fact that IL-3 and the T cell supernatant induce comparable levels of proliferation suggests that the NFS-60 cell line may be relatively insensitive to additional faσtors present in the T cell supernatant. Thus, NFS-60 cells were used to assay IL-3 during attempts to separate IL-3 from other MCGF activities by protein fractionation. The TCGF aσtivity of Cl.l supernatant was assessed using the IL-2 dependent T cell line, HT2. Saturatinq concentrations of Cl.l supernatant invariably stimulated 3 H-thymidine incorporation well below the maximum level achieved with recombinant IL-2 (Fig. 14D). Similar results were obtained with two other cell lines. The supernatant does not contain an inhibitory substance sinσe its addition to HT2 cultures containing IL-2 showed no reduction in H-thymidine inσorporation (Fig. 14D) . These results suggest Cl.l supernatant σontains a TCGF aσtivity distinct from IL-2. Biochemical evidence in support of this conclusion is presented below. Preliminary Chromatographic Fractionation of Cl.l Supernatant. Cl.l supernatant was fractionated by various chromatographic methods with the immediate goal of separating IL-3 from other MCGF activities. Prolifera¬ tion of NFS-60 cells was used to track IL-3 specifically against the background of total MCGF activity revealed by MC/9 proliferation. TCGF activity was assessed by HT2 cell proliferation. When Cl.l supernatant was fractionated by cation exchange chromatography at neutral pH, approximately 98% of the loaded protein, 97% of the IL-3 units (assessed by NFS-60 proliferation) and 1% of the TCGF units appeared in the flow-through (Fig. 15). Elution of bound protein with a gradient of sodium chloride released TCGF activity at 0.19M NaCl (fraction 60). A small but reproducible peak of TCGF activity also appeared at 1M NaCl. No further significant TCGF activity could be eluted at higher NaCl concentrations (up to 3M). A small amount of IL-3 also eluted in approximately the same region as the TCGF peak (detectable NFS-60 response). Only fractions corresponding to peak TCGF activity (fractions 59-61) and the flow-through (fractions 1-20) stimulated high levels of MC/9 proliferation, comparable to the unfractionated supernatant (arrows. Fig. 15). Since titration curves suqqested fractions 59-61 contained more of the high level MCGF aσtivity than did the flow-through, attempts were made to deplete further the IL-3 aσtivity co-eluting with the TCGF peak and again assess MC/9 proliferation levels. Fractions 59-61 were therefore re- chromatographed twice more under identical conditions. After the third column pass, more than 95% of the TCGF activity consistently eluted at 0.19 M NaCl. There was no measurable IL-3 (NFS-60) response) in the flow-through or in any of the column fractions. However, still co-eluting with the TCGF peak was an MCGF activity that now stimulated a plateau level of MC/9 proliferation that was significantly lower than that obtained with Cl..1 supernatant or I .-3 (see below). Since these fractions were devoid of measurable IL-3 activity (absence of NFS- 60 response), apparently the new MCGF by itself was incapable of stimulating MC/9 cells to the same degree as the crude T σell supernatant. To further attempt separation of MCGF from TCGF, the 3X fractionated material (fractions 59-61) was diluted 10X with 0.1% TFA in H 2 0 to pH 2 and loaded directly onto a C8 reverse phase column. Fig. 16A shows the elution profile of bound protein released with a qradient of acetonitrile, 0.1% in TFA. No MCGF or TCGF activity appeared in the flow-throuqh; both activities co-eluted at 37% acetonitrile (fractions 26-29). However, the saturating MCGF response was now only about one half that produced by IL-3 alone (Fiq. 17A) . When all fractions were re-assayed in the presence of saturatinq levels of IL-3, only fractions 26-29 showed an MC/9 proliferation response in excess of IL-3 alone (arrow. Fig. 16A) . In fact, the magnitude of the response was similar to that of the unfractionated supernatant itself (Fiq. 17A). Further, proliferation levels equivalent to those of unfractionated supernatant were also observed with fractions 26-29 were assayed in the presence of IL-3 containing flow-through or IL-3 purified form WEHI 3. Thus, the combination of IL-3 and the unique MCGF/TCGF stimulates levels of MC/9 proliferation characteristic of the original T cell supernatant. Altough reverse phase chromatography fails to resolve the MCGF from the TCGF, the TCGF was shown to be distinct from IL-2 by two criteria. First, when recombinant IL-2 in C0S-7 cell supernatants (IL-2 ) and supernatant from an IL-2 producing (ConA stimulated) murine T cell line (GF15-1) are both chromatographed on the same column under identical conditions, the TCGF activity elutes at 45% acetonitrile in a sharp peak that does not overlap with the 37% acetonitrile position markinq the Cl.l TCGF (Fiq. 16B). Second, the saturating response of HT2 cells to IL-2 is very different from that which characterizes the Cl.l supernatant (Fig. 16B, insert). Further, no obvious synergy was detected between the partially purified TCGF and IL-2 (Fig. 17B). The chromatofocussing pattern of Cl.l super¬ natant is shown in Fiq. 18. The IL-3 activity (NFS-60 response) displayed a very heteroqeneous profile, including aσtivity with pi values higher than 7.1, but the TCGF aσtivity appeared more homogeneous, with the major TCGF species (f action 12) showing an approximate pi of 6.2. This coincides- with the maximum MCGF activity (MC/9 response) after addition of saturating levels of IL-3. SDS PAGE of Cl.l Supernatant and of Partially Purified Factor. Figure 19 compares the electrophoretic profiles of unfractionated Cl.l supernatant (Fig. 19A) with that of the cation exchanged material, fractions 59-61 (Fiq. 19B). Protein was eluted directly from gel slices and activities were determined by proliferation. Both the σation exchange fraction and Cl.l supernatant show TCGF peaks: at 20 Kd and 15 Kd under non-reducing conditions, and at 21 Kd and 16 Kd under reducing conditions. Since the cation exchanged material was depleted of IL-3, only low levels of MC/9 proliferation were induced and this MCGF activity was still coinσident with TCGF peaks. Addition of saturating amounts of IL-3 to all fraσtions raised the MC/9 proliferation response to Cl.l supernatant levels only in the peak MCGF/TCGF fraσtions (arrows, Fig. 19B). IL-3 activity of the supernatant was greatest at the 24 Kd position, but also appears in broad peaks about the 20 Kd region. Significantly, MC/9 proliferation levels well above that of IL-3 occur at the 20 Kd position of electrophoresed supernatant (arrow, Fig. 19A). A silver-stained SDS gel of the most highly purified MCGF/TCGF material (fraction 28 of the reverse phase column) also showed prominent protein bands at 20 Kd and 15 Kd. Microgram quantities of the ' 20 kd form of the factor have been purified to homogeneity by successive fractionation of ConA-supernatants from activated Cl.l T- cells by (1) cation exchange chromatography, (2) Heparin Sepharose chromatography, and (3) c4 reverse phase chromatography as described previously. The final material shows only a single band at 20 kd by silver- stained SDS-PAGE, which corresponds to a single UV absorbing peak on the c4 elution .profile. Biological characterization of the purified factor confirmed its ability to stimulate low levels of T-cell and mast cell prolif eration and to enhance IL-3-dependent mast cell proliferation. As shown in Table V, the factor possesses several B-cell stimulatory activities ascribed to BSFl (Roehm, N.W. et al., J. Exp. Med. 169: 679-694 (1984); Howard, M. et al., Proc. Natl. Acad. Sci. U.S.A. 78: 5788 (1981); Roehm, N.W. et al . , Proc. Natl. Acad. Sci. U.S.A. 81: 6149-6153 (1984). First, it induces increased expression of la on resting B-cells as compared to the control polulation (Table V). Second, the factor, in conjunction with anti-Ig antibodies, induces significant [ H] -thymidine incorporation, indicating that it functions as an accessory factor in the stimulation of B- σell proliferation. Table V B-cell Stimulation Assays [ ] -thymidine incorporation I-A Source of anti-Ig treated B cells* induction** Unfractionated Cl.Lyl + 2 " /9 33,788 cpm ± 5,454 162.3 Supernatant Purified factor 44,125 cpm ± 2,775 157.1 Medium (negative control) 4,128 cpm ± 734 35.5 results reported as average cpm ± SEM of triplicate cultures supplemented with saturating ievels of factor. ** results reported as average relative mean fluorescence of triplicate cultures supplemented with saturatinq levels of factor. These polypeptides can exhibit several biological activities, differing in both the apparent function mediated and the type of cell affected. As described, these activities include synergy in the induction of proliferation of B-cells and mast cell lines, early activation of resting B-cells, stimulation of the proliferation of T-cells, and selective enhancement of IgG^ and IgE production of mitoqen- activated B-cells. Purification of the polypeptides to homogeneity has clarified the socpe of these activities. From the foregoing, it will be appreσiated that the purified proteins of the present invention provide those skilled in the art with novel therapeutic capabilities. The ability to produσe signifiσant quantities of these faσtors will permit improved in vitro σulture of selected mammalian cell lines, as well as an overall improved understanding of the mammalian immune response. cDNA clones pcD-2A-E3, pcD-46 (pcD-2Fl-13) , pcD-125, and yeast vector pMF-alpha8 have been deposited with the American Type Culture Collection, Rockville, MD, USA (ATCC), under accession numbers 53330, 53337, 67029, and 40140, respectively. These.deposits have been made under the Budapest Treaty (1977) on the International Recoqnition of the Deposit of Miσro-organisms for the purposes of Patent Procedure.

Claims: CLAIMS : 1. A protein comprising mammalian interleukin-4, preferably primate interleukin-4, rodent interleukin-4, or unqulate interleukin-4, in particular human interleukin-4; especially interleukin-4 havinq at least 9 x 10 7 units/mg of TCGF activity. 2. The compound of claim 3 wherein said interleukin-4 is human interleukin-4 and further exhibits at least one activity, preferably at least two, selected from MHC antiqen induction activity, Fσ-epsilon reσeptor induσtion aσtivity, GM-CSF stimulated granuloσyte colony growth potentiating activity, interleukin-2 TCGF potentiating activity, and IgG-^- and IgE-induction activity, and in particular is a glycosylated or unglycosylated polypeptide having a seguence of amino acids defined by the formula: X(His) - X(Lys) - X(Cys) - X(Asp) - X(Ile) - X(Thr) - X(Leu) - X(Gln) - X(Glu) - X(Ile) - X(Ile) - X(Lys) - X(Thr) - X(Leu) - X(Asn) - X(Ser) - X(Leu) - X(Thr) - X(Glu) - X(Gln) - X(Lys) - X(Thr) - X(Leu) - X(Cys) - X(Thr) - X(Glu) - X(Leu) - X(Thr) - X(Val) - X(Thr) - X(Asp) - X(Ile) - X(Phe) - X(Ala) - X(Ala) - X(Ser) - X(Lys) - X(Asn) - X(Thr) - X(Thr) - X(Glu) - X(Lys) - X(Glu) - X(Thr) - X(Phe) - X(Cys) - X(Arq) - X(Ala) - X(Ala) - X(Thr) - X(Val) - X(Leu) - X(Arg) - X(Gln) - X(Phe) - X(Tyr) - X(Ser) - X(His) - X(His) - X(Glu) - X(Lys) - X(Asp) - X(Thr) - X(Arg) - X(Cys) - X(Leu) - X(Gly) - X(Ala) - X(Thr) - X(Ala) - X(Gln) - X(Gln) - X(Phe) - X(His) - X(Arg) - X(His) - X(Lys) - X(Gln) - X(Leu) - X(Ile) - X(Arg) - X(Phe) - X(Leu) - X(Lys) - X(Arg) - X(Leu) - X(Asp) - X(Arg) - X(Asn) - X(Leu) - X(Trp) - X(Gly) - X(Leu) - X(Ala) - X(Gly) - X(Leu) - X(Asn) - X(Ser) - X(Cys) - X(Pro) - X(Val) - X(Lys) - X(Glu) - X(Ala) - X(Asn) - X(Gln) - X(Ser) - X(Thr) - X(Leu) - X(Glu) - X(Asn) - X(Phe) - X(Leu) - X(Glu) - X(Arg) - X(Leu) - X(Lys) - X(Thr) - X(Ile) - X(Met) - X(Arg) - X(Glu) - X(Lys) - X(Tyr) - X(Ser) - X(Lys) - X(Cys) - X(Ser) - X(Ser) wherein: X(Ser) represents the qroup consisting of Ser, Thr, Gly, and Asn; X(Arg) represents the group consisting of Arg, His, Gin, Lys, and Glu; X(Leu) represents the group consisting of Leu, He, Phe, Tyr, Met, and Val;. X(Pro) represents the group consisting of Pro, Gly, Ala, and Thr; X(Thr) represents the group consisting of Thr, Pro, Ser, Ala, Gly, His, and Gin; X(Ala) represents the qroup σonsisting of Ala, Gly, Thr, and Pro; X(Val) represents the qroup consistinq of Val, Met, Tyr, Phe, He, and Leu; X(Gly) represents the group consisting of Gly, Ala, Thr, Pro, and Ser; X(He) represents the group consisting of He, Met, Tyr, Phe, Val, and Leu; X(Phe) represents the group consisting of Phe, Trp, Met, Tyr, He, Val, and Leu; X(Tyr) represents the group consisting of Tyr, Trp, Met, Phe, He, Val, and Leu; X(His) represents the group consisting of His, Glu, Lys, Gin, Thr, and Arg; X(Gln) represents the group consisting of Gin, Glu, Lys, Asn, His, Thr, and Arg; X(Asn) represents the qroup consisting of Asn, Glu, Asp, Gin, and Ser; X(Lys) represents the group consisting of Lys, Glu, Gin, His, and Arg; X(Asp) represents the group consisting of Asp, Glu, and Asn; X(Glu) represents the group σonsisting of Glu, Asp, Lys, Asn, Gin, His, and Arg; and X(Met) represents the group σonsisting of Met, Phe, He, Val, Leu, and Tyr. 3. The σompound of claim 2 wherein said glycosylated or unglycosylated polypeptide is up to 10-fold substituted, in particular wherein said polypeptide is not more than 1-fold substituted within the region defined by amino acid residues 3-29 inclusive, 35-66 inclusive, 78-87 inclusive, 98-99 inclusive, and 105-125 inclusive. 4. The compound of claim 2 wherein: X(Arg is Arg; X(Leu represents the group consisting of Leu, He, and Met; X(Pro is Pro; X(Ala is Ala; X(Val is Val; X(He represents the group σonsisting of He, Met, and Leu; X (Phe is Phe X (Tyr is Tyr X (His is His X (Gln is Gin X (Asn is Asn X (Lys is Lys X (Asp is Asp X (Glu is Glu; and X (Met represents the group σonsisting of Met, He, and Leu; in particular wherein said polypeptide is up to 10-fold substituted, especially not more than 1-fold substituted within the region defined by amino acid residues 3-29 inσlusive, 35-66 inclusive, 78-87 inclusive, 98-99 inclusive, and 105-125 inclusive, preferably wherein said polypeptide is defined by the formula: His-Lys-Cys-Asp-Ile-Thr-Leu-Gln-Glu-He- Ile-Lys-Thr-Leu-Asn-Ser-Leu-Thr-Glu-Gln- Lys-Thr-Leu-Cys-Thr-Glu-Leu-Thr-Val-Thr- Asp-Ile-Phe-Ala-Ala-Ser-Lys-Asn-Thr-Thr- . Glu-Lys-Glu-Thr-Phe-Cys-Arg-Ala-Ala-Thr- Val-Leu-Arg-Gln-Phe-Tyr-Ser-His-His-Glu- Lys-Asp-Thr-Arg-Cys-Leu-Gly-Ala-Thr-Ala- Gln-Gln-Phe-His-Arg-His-Lys-Gln-Leu-He- Arg-Phe-Leu-Lys-Arg-Leu-Asp-Arg-Asn-Leu- Trp-Gly-Leu-Ala-Gly-Leu-Asn-Ser-Cys-Pro- Val-Lys-Glu-Ala-Asn-Gln-Ser-Thr-Leu-Glu- Asn-Phe-Leu-Glu-Arg-Leu-Lys-Thr-He-Met- Arg-Glu-Lys-Tyr-Ser-Lys-Cys-Ser-Ser. 5. The compound of claim 1 seleσted from the glyσosylated or unglycosylated 10-fold inserted, 10-fold deleted, and 10-fold substituted polypeptides of the formula: X(His) - X(Lys) - X(Cys) - X(Asρ) - X(He) - X(Thr) - X(Leu) - X(Gln) - X(Glu) - X(He) - X(Ile) .- X(Lys) - X(Thr) - X(Leu) - X(Asn) - X(Ser) - X(Leu) - X(Thr) - X(Glu) - X(Gln) - X(Lys) - X(Thr) - X(Leu) - X(Cys) - X(Thr) - X(Glu) - X(Leu) - X(Thr) - X(Val) - X(Thr) - X(Asp) - X(He) - X(Phe) - X(Ala) - X(Ala) - X(Ser) - X(Lys) - X(Asn) - X(Thr) - X(Thr) - X(Glu) - X(Lys) - X(Glu) - X(Thr) - X(Phe) - X(Cys) - X(Arg) - X(Ala) - X(Ala) - X(Thr) - X(Val) - X(Leu) - X(Arg) - X(Gln) - X(Phe) - X(Tyr) - X(Ser) - X(His) - X(His) - X(Glu) X(Lys) - X(ASp) - X(Thr) - X(Arg) - X(Cys) - X(Leu) X(Gly) - X(Ala) - X(Thr) - X(Ala) - X(Gln) - X(Gln) X(Phe) - X(His) - X(Arg) - X(His) - X(Lys) - X(Gln) X(Leu) - X(He) - X(Arg) - X(Phe) - X(Leu) - X(Lys) X(Arg) - X(Leu) - X(Asp) - X(Arg) - X(Asn) - X(Leu) X(Trp) - X(Gly) - X(Leu) - X(Ala) - X(Gly) - X(Leu) X(Asn) - X(Ser) - X(Cys) - X(Pro) - X(Val) - X(Lys) X(Glu) - X(Ala) - X(Asn) - X(Gln) - X(Ser) - X(Thr) X(Leu) - X(Glu) - X(Asn) - X(Phe) - X(Leu) - X(Glu) X(Arg) - X(Leu) - X(Lys) - X(Thr) - X(He) - X(Met) X(Arg) - X(Glu) - X(Lys) - X(Tyr) - X(Ser) - X(Lys) X(Cys) - X(Ser) - X(Ser) wherein: X(Ser) represents the group consisting of Ser, Thr, Gly, and Asn; X(Arg) represents the group σonsisting of Arg, His, Gin, Lys, and Glu; X(Leu) represents the group σonsisting of Leu, He, Phe, Tyr, Met, and Val; X(Pro) represents the group consisting of Pro, Gly, Ala, and Thr; X(Thr) represents the group consisting of Thr, Pro, Ser, Ala, Gly, His, and Gin; X(Ala) represents the group consisting of Ala, Gly, Thr, and Pro; X(Val) represents the group consisting of Val, Met, Tyr, Phe, He, and Leu; X(Gly) represents the group consisting of Gly, Ala, Thr, Pro, and Ser; X(He) represents the group consisting of He, Met, Tyr, Phe, Val, and Leu; X(Phe) represents the group σonsisting of Phe, Trp, Met, Tyr, He, Val, and Leu; X(Tyr) represents the group consisting of Tyr, Trp, Met, Phe, He, Val, and Leu; X(His) represents the group consisting of His, Glu, Lys, Gin, Thr, and Arg; X(Gln) represents the group consisting of Gin, Glu, Lys, Asn, His, Thr, and Arg; X(Asn) represents the group consisting of Asn, Glu, Asp, Gin, and Ser; X(Lys) represents the group σonsisting of Lys, Glu, Gin, His, and Arg; X(Asp) represents the group σonsisting of Asp, Glu, and Asn; X(Glu) represents the group consisting of Glu, Asp, Lys, Asn, Gin, His, and Arg; and X(Met) represents the group consisting of Met, Phe, He, Val, Leu, and Tyr; espeσially wherein X(Ser) is Ser; X(Arg) represents the group σonsisting of Arg, His, and Lys; X(Leu) represents the group σonsisting of Leu, He, Phe, and Met; X(Pro) represents the group σonsisting of Pro and Ala; X(Thr) is Thr; X(Ala) represents the group consisting of Ala and Pro; X(Val) represents the group consisting of Val, Met, and He; X(Gly) is Gly; X(He) represents the group consisting of He, Met, Phe, Val, and Leu; X(Phe) represents the group consisting of Phe, Met, Tyr, He, and Leu; X(Tyr) represents the group consisting of Tyr and Phe; -Ill- X(His represents the group consisting of His, Gin, > and Arq; X(Gln represents the qroup consisting of Gin, Glu, • and His; X(Asn represents the qroup consistinq of Asn and Asp; X(Lys represents the qroup consistinq of Lys and Arg; X(Asp represents the group consisting of Asp and Asn; X(Glu represents the group consisting of Glu and Gin; and X(Met) represents the group consisting of Met, Phe> He, Val, and Leu; in particular wherein said polypeptide is not more than 2-fold inserted, not more than 2-fold deleted, or not more than 2-fold substituted within the region defined by amino aσid residues 3-29 inσlusive, 35-66 inσlusive, 78- 87 inclusive, 98-99 inclusive, and 105-125 inclusive; especially human interleukin-4 mutein A , human interleukin-4 mutein IS (Gly-Asn-Phe-Val-His-Gly) , or human interleukin-4 mutein IS (Ala-Glu-Phe) . 6. The compound of claim 2 wherein said rodent interleukin-4 is murine interleukin-4, in particular murine interleukin-4 that further exhibits at least one activity, preferably at least two, selected from the group consisting of MCGF activity, MHC antigen induction activity, Fc-epsilon receptor induction activity, GM-CSF stimulated granulocyte colony growth potentiating activity, interleukin-2 TCGF potentiating activity, and IgG-^- and IgE-induction activity, especially murine interleukin-4 as defined by the formula: His-I le-His-Gly-Cys-Asp-Lys-Asn-His-Leu- . Arg-Glu-Ile-Ile-Gly-Ile-Leu-Asn-Glu-Val- Thr-Gly-Glu-Gly-Thr-Pro-Cys-Thr-Glu-Met- Asp-Val-Pro-Asn-Val-Leu-Thr-Ala-Thr-Lys- Asn-Thr-Thr-Glu-Ser-Glu-Leu-Val-Cys-Arg- Ala-Ser-Lys-Val-Leu-Arg-Ile-Phe-Tyr-Leu- Lys-His-Gly-Lys-Thr-Pro-Cys-Leu-Lys-Lys- Asn-Ser-Ser-Val-Leu-Met-Glu-Leu-Gln-Arg- Leu-Phe-Arg-Ala-Phe-Arg-Cys-Leu-Asp-Ser- Ser-He-Ser-Cys-Thr-Met-Asn-Glu-Ser-Lys- Ser-Thr-Ser-Leu-Lys-Asp-Phe-Leu-Glu-Ser- Leu-Lys-Ser-Ile-Met-Gln-Met-Asp-Tyr-Ser . 7. A pharmaσeutiσal σomposition for stimulating the immune system comprising a therapeutically compatible carrier and an effective amount of human interleukin-4, in particular wherein said human interleukin-4 is a compound of σlaim 3 or σlaim 4. 8. A nuσleiσ acid comprising a sequence of nucleotide bases capable of coding for a polypeptide exhibiting mammalian interleukin-4 aσtivity and at least one additional aσtivity seleσted from the group consisting of MHC antigen induction activity, Fc-epsilon reσeptor induction aσtivity, GM-CSF stimulated granuloσyte colony growth potentiating activity, interleukin-2 TCGF potentiating activity, and IgG- ] _- and IgΞ-induction aσtivity, preferably a sequenσe of nuσleotide bases that is effeσtively homologous to a sequenσe of DNA in a σDNA insert of a veσtor seleσted from pσD-46, pσD-125, and pσD-2A-E3, in particular wherein said effeσtively homologous sequenσe is at least fifty perσent homologous and preferably at least seventy- five percent homologous to said sequence of DNA in said cDNA insert of said veσtor seleσted from said group σonsisting of pσD-46, pcD-125, and PCD-2A-E3. 9. A σompound selected from the group consisting of nucleiσ aσids σomprising a sequenσe of σodons σapable of σoding for the polypeptides any of σlaims 3 to 5, in particular a sequence of codons defined by the sequence of bases from position 64, preferably position 130, to position 522 in Figure IB, especially the sequence of codons is defined by the following formula: CAC AAA TGΓ GAC ATC ACT CΓG CAA GAA ATC ATC AAA Λ C G AAC TCG TTA ACC GAA CAG AAA ACC CΓG TGC ACC GAG CTC ACT GTT ACT GAT ATC TΓC GCT GCT TCC AAA AAC ACT ACT GAA AAA GAA ACT TΓC TGC AGA GCT GCT Λ GΓΓ CTG CGT CAG TIC TA TCT CAC CAC GAA AAA GA ACG CCT CT CTC Q QCC ACT QCG CAG CAG TTC Q CCT CAC AAA CAG CTG ATC AGA TTC CTG AAA CGT CTA GfC CGT AAC CTG GG GGC CTG GCC GGC CTG AAC TCT TCT CCG GTT AAA GAA GCT AAC CAG TCG ACT CTG GAA AAC TTC CTC GAG CGT CTG AAA ACC ATC ATG CGT GAA AAG TAC TCT AAA TGC TCT TCT. 10. A method for producing a polypeptide exhibiting human interleukin-4 activity, the method comprising the steps of: construσting a veσtor σomprising a nuσleotide sequenσe σoding for said polypeptide, wherein the nuσleotide sequenσe is σapable of being expressed by a host σontaining the vector and wherein the nucleotide sequence is at least 50 percent homologous to the insert of pcD-125; inσorporating the veσtor into the host; and maintaining the veσtor-σontaining host under conditions suitable for expression of the nucleotide sequenσe into said polypeptide; in particular a method wherein said nucleotide sequence is selected from those defined by claim 9, and wherein said host is preferably a mammalian cell. 11. A cell selected from yeast cells, bacterial cells, and mammalian σells, preferably from Esσherichia coli cells, Saccharomyces σerevisiae σells, COS 7 monkey σells, σhinese hamster ovary σells, mouse L cells, and mouse myeloma cells, said cell being transformed or transiently transfected by a vector comprising a nucleic acid sequence defined by claim 9. 12. A vector capable of transforming a bacterial host, the vector comprising a nuσleiσ aσid sequenσe defined by claim 5, in particular a vector selected from plN-III-ompA2, TRPCll, and TAC-RBS, or a vector capable of transforming a yeast host and comprising a nucleic acid sequence defined by claim 5, e.g., the vector pMF-alρha8, or a veσtor σapable of transforming or transiently transfeσting a mammalian host, the veσtor σomprising a nucleic acid sequence defined by claim 5, preferably consisting of a pcD plasmid, e.g., pcD-125 or pcD-46. 13. A method for treating bare lymphocyte syndrome σomprising the step of stimulating expression of σlass II HLA-DR antigens on B σells by administering an effeσtive dose of the pharmaceutical composition of σlaim 7.

Inventors: ARAI KEN-ICHI, LEE FRANK, MOSMANN TIMOTHY, RENNICK DONNA, SMITH CRAIG, YOKOTA TAKASHI, ARAI, KEN-ICHI, LEE, FRANK, MOSMANN, TIMOTHY, RENNICK, DONNA, SMITH, CRAIG, YOKOTA, TAKASHI
Applicants: SCHERING BIOTECH CORP, SCHERING-BIOTECH CORPORATION
Application year: 1986
Main classification: C07K15
IPC codes: A61K38, A61K39, A61P37, C07H21, C07K1, C07K14, C12N1, C12N5, C12N15, C12P21, C12R1