ANTIGLAUCOMA COMPOSITIONS

Abstract: Ophthalmological pharmaceutical compositions comprising an angiotensin converting enzyme (ACE) inhibitor and a beta adrenergic blocker useful for combination therapy of glaucoma.

Description: ANTIGLAUCOMA COMPOSITIONS BACKGROUND OF THE INVENTION Glaucoma is an ocular disease complex associated with an elevated pressure within the eye (i.e., intraocular pressure, IOP) . As a result of the elevated IOP, damage to the optic nerve head resulting in irreversible loss of visual function may ensue. Untreated, this condition may eventually lead to blindness. Ocular hypertension, i.e., the condition of elevated intraocular pressure without optic nerve head damage or characte istic glaucomatous visual field loss, is now believed by the majority of ophthalmologists to represent the earliest phase in the onset of glaucoma. A number of the drugs presently employed to treat glaucoma are not entirely satisfactory, particularly in the earliest course of the disease when the side effects they produce are often worse than the symptoms of the disease. Epineph ine, used as a topical solution, must be utilized cautiously in patients with high blood pressure, diabetes, hyperthyroidis and cerebral artereosclerosis due to the possibility of systemic action. SUBSTIT Timolol, a clinically utilized, topically applied agent for lowering intraocular pressure, must be used with caution in patients in whom Beta-adrenergic blockade may be undesirable. Systemic absorption of topically administered timolol and systemic Beta-blockade are responsible for the contraindication of timolol therapy for glaucoma in patients with compromised pulmonary function and in patients who cannot tolerate its systemic cardiovascular action. Pilocarpine, a topical drug, although considered systemically harmless and quite effective, may cause considerable local difficulties. Pupillary constriction causes the eye to lose its ability to adapt from light to dark. Accomodation may become stimulated so that the patient's refraction is sometimes incorrect and vision becomes blurred. The drug itself may cause a local vasodilation and red eyes. Irritation is common. Carbonic anhydra-se inhibitors have been used systemically but they have a number of disadvantages. While effective in lowering intraocular pressure, they often cause a numbness and tingling, gastrointestinal upsets and, frequently, depression, lethargy, a loss of appetite, and general malaise. European Patent Application 31400326.5, Publication number 36,351 attempts to overcome these difficulties by the topical administration of an alkali metal salt of a carbonic anhydrase inhibitor. The European published patent application Nos. 99239 and 114333 describe certain angiotensin converting enzyme inhibitors as being useful in controlling the intraocular pressure. The present invention provides a new approach in the therapy for reducing and controlling the elevated intraocular pressure associated with glaucoma. The invention sought to be patented in its pharmaceutical composition aspect is a topical ophthalmoloqically acceptable composition useful for reducing and/or controllinq the elevated intraocular pressure associated with qlaucoma which comprises a pharmaceutically acceptable anqiotensin convertinq enzyme inhibitor and a pharmaceutically acceptable beta adrenergic blocker in combination with an ophthalmoloqically acceptable carrier. Preferably the composition comprises for an amount of from .005 υg to 50 uq of the ACE inhibitor an amount of from 5 μg to 500yg of the beta adrenerqic blocker. According to a modification of the invention, pharmaceutical compositions are comprised which contain either the beta adrenerqic blocker or the ACE inhibitor for combined use of these active components. The invention souqht to be patented in a further aspect is a kit comprisinq in separate containers in a sinqle packaqe pharmaceutical compositions for use in combination to reduce and control the elevated intraocular pressure associated with qlaucoma in humans which comprises in one container a topical ophthalmoloqical pharmaceutical composition comprisinq an anqiotensin convertinq enzyme inhibitor and in the second container, a topical ophthalmoloqical pharmaceutical composition comprisinq a beta adrenerqic blocker. Preferably the compositions contain the active components in such concentration that the one composition provides 0.005 to 50uq of the anqiotensin converting enzyme inhibitor per unit dose and the other composition provides 0.5 to 500uq of a beta adrenergic blocker per unit dose. The invention sought to be patented in its pharmaceutical method aspect is a method for reducing and controlling the elevated intraocular pressure associated with glaucoma in a human which method comprises administering to said human an effective amount of the above-defined pharmaceutical composition. Examples of angiotensin converting enzyme (ACE) inhibitors are those disclosed in the following patents and published patent applications: (a) U.S. Patent No. 4,105,776 to Ondetti et al. discloses proline derivatives which are angiotensin converting enzyme (ACE) inhibitors and have the general formula wherein R a is hydroxy, NH * 2 or lower alkoxy; R ] _ a and R 4 a each is hydrogen, lower alkyl, phenyl or phenyl-lower alkyl; R 2 a is hydrogen, lower alkyl, phenyl, substituted phenyl wherein the phenyl substituent is halo, lower alkyl or lower alkoxy, phenyl-lower alkyl, diphenyl-lower alkyl, triphenyl-lower alkyl, lower alkylthiomethyl, phenyl-lower alkylthiomethyl, lower alkanoyl-amidomethyl. SUBSTITUTE GWxξST R 6 a -S- or R 7 a ; R3 a is hydrogen, hydroxy or lower alkyl; R 5 a is lower alkyl, phenyl or phenyl-lower alkyl; Rg a is lower alkyl, phenyl, substituted phenyl (wherein the phenyl substituent is halo, lower alkyl or lower alkoxy) , hydroxy-lower alkyl or amino (carboxy)-lower alkyl; R a is R a - )jja- S(0) p a M a is 0 o • S; m a is 1 to 3; n a and p a each is 0 to 2. (b) European Patent Application 97050 published December 28, 1983 discloses phosphinylalkanoyl substituted prolines which have the formula SUBSTITUTE SHEΞT • τ_ is- alkyl, aryl, aralkyl, cycloalkyl or cycloalkylalkyl; R 2 is cycloalkyl, 3-cyclohexenyl or 2-alkyl-3- cyclohexenyl; R R3 3 b " iiss aallkkyyll,, cycloalkyl or phenyl; R 4 b is H or alkyl; one of R 5 b and R g b is H and the other is alkyl-X b -, phenyl-X , alkoxy, phenoxy, phenyl, cycloalkyl, alkyl or phenylalkyl; or R 5 b and R 6 b together form -X b CH 2 CH 2 X b -, X b is S, 'aryl' is phenyl opt. substituted by halo, alkyl, alkoxy, alkylthio, OH, alkanoyl, N0 2 , amino, dialkylamino and/or CF3; alkyl has 1-lOC, cycloalkyl 3-7C, alkoxy 1-8C and alkanoyl 2-9C. (c) European Patent Application 83172 published July 6, 1983 discloses N-substituted acetyl- - proline derivatives which are said to be angiotensin converting enzyme inhibitors and have the formula R^ and R 2 G are H, 1-7C alkyl, 1-7C haloalkyl, (C H 2)m c-D c . 1-7C aminoalkyl or a group of formula: SUBSTITUTE SHEET D is cycloalkyl, furyl, thienyl or pyridinyl; A σ is (CH 2 ) n -CHR 21 c , NR 22 C -CHR 23 C or 0-CHR 23 c ; n * is 0 or 1; q σ is 0-7; R 21 C is H, 1-7C alkyl, 1-7C haloalkyl, benzyl or phenethyl; R 2 c is H or 1-7C alkyl; R 23 c is H, 1-7C alkyl, 1-7C haloalkyl or (CH 2 ) r cD 1 c ; D^ c is phenyl, 4-hydroxyphenyl, 3,4- dihydroxyphenyl, 3-indolyl, 4-imidazolyl, NH 2 , SH, 1-7C alkylthio, guanidino or CONH 2 ; X c is a group of formula (II) C -(V) C , or NR 4 C - CHR 5 c -COOR 6 σ ; CV) SUBSTITUTE SHEET where Rg σ and Rg c are H and R-j c is as defined below; R 9 C and R 7 C are H and Rg c is as defined below; R- 7 C and Rg c are H and Rg C is as defined below; R 9 C and Rg C are H and -CHR 7 C - is replaced by -CR 1Q R 10 ; Rg c is H and R 7 C + Rg c form a double bond; R 9 C is H and R 7 C + Rg C complete a fused benzene ring; or Rg c is H and R 9 C + R 7 C complete a fused benzene ring; E ,c and E ,,c are H or E ,c + E ,,c complete a fused benzene ring; R 7 C is H, 1-7C alkyl, halogen, keto, OH, 2-8C alkanoyl-amino, N 3 , NH 2 , NR 19 C R 20 C , (CH 2 ) πl σ -D c , 0-CONR 15 c R 15 σ , 1-7C alkoxy, 1-7C alkylthio, or a group of formula (VI) c , (VII) c or (.VIII) c : (VI) (VII) B' c is a bond or it is 0 or S;Rg C is keto, halogen, 0-CONR 15 c R 15 c , 1-7C alkoxy, 1-7C alkylthio, or a group (VII) c or (VIII) c in which R 9 C is '.otto or a group (VII) c in which B* c is a bond R l0 c is halogen or Y c R 1 g C ; l ll , 'i ] _ , R 12 and R*2_ 2 are H or 1-7C alkyl. or R U c is a group of formula (IX) c and the other 3 are H; SUBSTITUTE SHEET (IX) R 13 σ is H, 1-4C alkyl, 1-4C alkoxy, 1-4C alkylthio, Cl, Br, F, CF 3 , OH, Ph, PhO, PhS or PhCH 2 ; R 14 c is H, 1-4C alkyl, 1-4C alkoxy, 1-4C alkylthio, Cl, Br, F, CF or OH; m c is 0-3; p c is 1-3 but it is 2 or 3 only when R ] _ 3 C or R 1 C is H, Me, MeO, Cl or F; R 15 c is H or 1-4C alkyl; Y c is 0 or S; R 16 c is 1-4C alkyl or group. (VII) c in which B' c is a bond; or the two ] _ C groups complete a 5- or 6-membered ring in which the C atoms may each bear 1 or 2 alkyls having 1-4C; R C is H, 1-7C alkyl, cycloalkyl or (CH ) r c Ph; R 5 C is H, 1-7C alkyl or (CH 2 ) r c -D 1 c ; r G is 1-4; R 3 C and Rg C are H, 1-7C alkyl, PhCH 2 , PhCH or CHR 17 c -0-COR 18 c ; R 17 c is H, 1-7C alkyl or Ph; R 18 c is H, 1-7C alkyl, 1-7C alkoxy or Ph; or R 17° + R 18 G form (CH 2 ) 2' (CH 2 ) 3' "CH= H or o-phenylene; R 19° -■-- 1~7C alkyl, benzyl or phenethyl; and R 2 o C is H or chosen as for ^g 0 - (d) European Patent Application 0 012 401 published June 25, 1980 discloses carboxyalkyl dipeptide derivatives which are said to be angiotensin converting enzyme inhibitors and have the formula SUBSTITUTE SHEET wherein R d and R 6d are the same or different and are hydroxy, lower alkoxy, lower alkenoxy, dilower alkylamino lower alkoxy (dimethylaminoethoxy) , acylamino lower alkoxy (acetylaminoethoxy) , acyloxy lower alkoxy (pivaloyloxymethoxy) , aryloxy, such as phenoxy, arloweralkoxy, such as benzyloxy, substituted aryloxy or substituted arloweralkoxy wherein the substituent is methyl, halo or methoxy, amino, loweralkylamino, diloweralkylamino, hydroxyamino, arloweralkylamino such as benzylamino; R ld is hydrogen, alkyl of from 1 to 20 carbon atoms which include branched and cyclic and unsatruated (such as allyl) alkyl groups, substituted loweralkyl wherein the substituent can be halo, hydroxy, lower alkoxy, aryloxy such as phenoxy, amino, diloweralkylamino, acyamino, such as acetamido and benzamido, arylamino, guanidino, imidazolyl, indolyl, mercapto, loweralkylthio, arylthio such as phenylthio, carboxy or carboxamido, carboloweralkoxy, aryl such as phenyl or naphthyl, substituted aryl such as phenyl wherein the substituent is lower alkyl, lower alkoxy or halo, arloweralkyl, arloweralkenyl, heteroarlower alkyl or heteroarlower alkenyl such as benzyl, styryl or indolyl ethyl, substituted arloweralkyl, substituted arloweralkenyl, substituted heteroarlower alkyl, or substituted heteroarlower alkenyl, wherein the SUBSTITUTE SHEET substituent(s) is halo, dihalo, lower alkyl, hydroxy, lower alkoxy, amino, aminomethyl, acylamino (acetylamino or benzoyla ino) diloweralkylamino, loweralkylamino, carboxyl, haloloweralkyl, cyano or sulfonamido; arloweralkyl or heteroar'loweralkyl substituted on the alkyl portion by amino or acylamino (acetylamino or benzoylamino) ; R and R' d are the same or different and are hydrogen or lower alkyl; R is hydrogen, lower alkyl, phenyl lower alkyl, aminomethyl phenyl lower alkyl, hydroxy phenyl lower alkyl, hydroxy lower alkyl, acylamino lower alkyl (such as benzoylamino lower alkyl, acetylamino lower alkyl), amino lower alkyl, dimethylamino lower alkyl, halo- lower alkyl, guanldino lower alkyl, imidazolyl lower alkyl, indolyl lower alkyl, mercapto lower alkyl, lower alkyl thio lower alkyl; R is hydrogen or lower alkyl; 5r_ is hydrogen, lower alkyl, phenyl, phenyl lower alkyl, hydroxy phenyl lower alky, hydroxy lower alkyl, amino lower alkyl, guanidino lower alkyl, imidazolyl lower alkyl, indolyl lower alkyl, mercapto lower alkyl or lower alkylthio lower alkyl; R and R d may be connected together to form an alkylene bridge of from 2 to 4 carbon atoms, an alkylene bridge of from 2 to 3 carbon atoms and one sulfur atom, an alkylene bridge of from 3 to 4 carbon atoms containing a double bond or an alkylene bridge as above substituted with hydroxy, loweralkoxy, lower alkyl or dilower alkyl; SUBSTITUTE SKEHT and the pharmaceutically acceptable salts thereof. (d) U.S. Patent No. 4,462,943 to Petrillo et al. discloses carboxyalkyl amino acid derivatives of substituted prolines which are angiotensin converting enzyme inhibitors and have the formula 0 R ® R ® ■ ' I R e -C - C - NH - CH - C - R e 4 i I R® I 2 O R 4 is a substituted proline of the formula tg e is halogen, keto, azido, • NH- NE- ?C-lower alkyl , SUBSTITUTE SHEET a 1- or 2-naphthyl of the f ormula e-cycloalkyl, a 1- or 2-naphthyloxy of the formula m rrte -S-lower alkyl, SUBST TUTE SHEET or a 1- or 2-naphthylthio of the formula l 7 e is keto, halogen . (R 10 } ne or 1- or 2-naphthloxy of the formula -S-lower alkyl . SUBSTITUTE SHEET ISA/EP or a 1- or 2-naphthylthio of the formula is keto or Rg e is halogen or -Y e -Rτ_ 3 e . m e is zero, one, two, or three. R 10 θ ~- s hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, hydroxy, phenyl, phenoxy, phenylthio, or phenylmethyl, R-- e is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, bromo, fluoro, trifluoromethyl, or hydroxy. n e is one, two or three provided that n e is more than one only if R***_g e or R- ® is hydrogen, methyl, methoxy, chloro, or fluoro. R 12 e is hydrogen or lower alkyl of 1 to 4 carbons, Y e is oxygen or sulfur, R, 3 e is lower alkyl of 1 to 4 carbons ^ , SUBSTITUTE SHEET or the R ] _ 3 e group join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a lower alkyl of 1 to 4 carbons, or a di (lower alkyl of 1 to 4 carbons) substituent, R e and Rg e are independently selected from hydroxy, lower alkoxy, di (lower alkyl)-amino- lower alkoxy, such as dimethylaminoethoxy, lower alkyl-carbonyl-amino-lower alkoxy, such as acetylaminoethoxy, lower alkyl-carbonyloxy-lower alkoxv. such as pivaloyloxy ethoxy. wherein m ® , n e and R ] _ Q e are as defined above, amino, lower alk l-amino, di (lower alkyl)-amino, hydroxyamino, benzylamino, or phenethylamino, R**L ® is hydrogen, lower alkyl. halo substituted lower alkyl, hydroxy substituted lower alky, -(CH 2 ) 8 β -cycloalkyl, -(CH 2 ) q ® carboxy, -(CH 2 ) g € S-lower alkyl. SUBSTITUTE SHEET -(CH 2 ) e -guanidinyl, -(CH 2 ) -NH 2 , -(CH,) ® - (lower alkyl) 2 , -(CH 2 ) -NH-C-lower alkyl, (CH 2 ) -lower alkoxy, - (CH 2> ( R- 1 ,? Q )) n e-(CH 2 ) δ C-NH -(CH 2 ) e -C-lower alkoxy, or SUBSTITUTE SHEET wherein m e , n e , **_ Q e and R*LI ® are as defined above, R* j _ 4 ® is lower alkyl, cycloalkyl, or and q ® is an integer from 1 to 4, R 2 ® is hydrogen or lower alkyl, R 3 e is hydrogen, lower alkyl, halo substituted lower alkyl, hydroxy substituted lower alkyl, -(CH 2 )q e -NH 2 , -(CH 2 )q e -N-(lower alkyl) 2 , -(CH 2 )q e guanidinyl, -(CH 2 )q e -SH, -(CH 2 )q ® -S- (CH 2 ) -NH- ?C-lower alkyl, or wherein R ±Q Q , n e and q e are as defined above< SUBSTITUTE SHEE I - S _H/t_.ir (f) U.S. Patent No. 4,470,973 to Natarajan et al. discloses substituted peptides which are angiotensin converting enzyme inhibitors and have the formula V_» R xW f R A-.x f 0 VJ , E — CH— C— CH — N— CH- C— X E NH I C=0 rf is an amino or imino acid of the formula H SUBSTITUTE SHEET R 7 is hydrogen, lower alkyl, halogen, keto, hydroxy -NH-C-lower alkyl azido, amino. SUBSTITUTE SHEET a 1- or 2-naphthyl of the formula k 15 -0-C-N< f , -O-lower alkyl, R ιs a 1- or 2-naphthyloxy of the formula -S -lower alkyl . or a 1- or 2-naphthylthio of the formula Rg E is keto, halogen. -0-lower alkyl, a 1- or 2-naphthyloxy of the formula -S-lower alkyl « ■ >. ;??< •* " *■ •- UBSTITUTE S ISA/SP or a 1- or 2-naphthylthio of the formula Rg is keto or R 10 is halogen or -Y -Ri . f f f f R ll ' R ll' ' R 12 and R 12' are independently selected from hydrogen and lower alkyl or R---i t f f ■ _ ■ . R 12 an< ^ R 12' are hydrogen and ~~ -n is R* ] _ 3 is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, hydroxy, phenyl, phenoxy, phenylthio, or phenyl ethyl. _" • . » • " * !» •• _ *»v JB3T1TUTE SHE SSA/EP R* ] _ 4 is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, or hydroxy. m E is zero, one, two, three, or four. p is one, two or three provided that p is more tthhaann oonnee oonnllyy iiff RR * *LL 33 oorr RR-- jj __ 44 iiss hhyy<drogen, methyl, methoxy, chloro, or fluoro. R 1 - j _ 5 5 fE iiss hydrogen or lower alkyl of 1 to 4 carbons. Y Y EE iiss ooxx;ygen or sulfur. Rτ_ is lower alkyl of 1 to 4 carbons. or the -^ groups join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a lower alkyl of 1 to 4 carbons or a di (lower alkyl of 1 to 4 carbons) substituent. R is hydrogen, lower alkyl. 2. m (CH 2 ) ^-cycloalkyl . _> SUBSTITUTE SK ISA -g E is hydrogen, lower alkyl. - ( CH,) " H 2 -(CH 2 )J-SH,- (CH 2 )J-S-lower alkyl. l 2'r -(CH, -NH 2 * r r is an integer from 1 to 4. R- j _9 is lower alkyl, benzyl, or phenethyl. R 2Q is hydrogen, lower alkyl, benzyl or phenethyl. R is hydrogen, lower alkyl, cycloalkyl. -(CH 2 ) 2 -NH 2 , -(CH 2 ) 3 -NH 2 , -(CH 2 ) 4 -NH 2 , -(CH 2 ) 2 -OH, -(CH 2 ) 3 -OH, -(CH 2 ) 4 -OH, -(CH 2 ) 2 -SH, -(CH 2 ) 3 -SH, or -(CH 2 ) 4 -SH. SUBSTITUTE S Rι E is> hydrogen, lower alkyl, halo substituted lower alkyl. - ( CH 2) J N, -(CH 2 )f r -N..H.. 2 ,- _ i ( nCτHt 2 " ) . r --SH, N i H (CH,)^-OH,-(CH 2 )J-S-lower alkyl, provided that R-x - is hydrogen only if R ~~ is other than hydrogen. R 2 f is SUBSTITUTE SHEE -_ _ * *-»_> _ά._ J R 3 is- hydrogen, lower alkyl lower alkyl ,-(CH ) -(CH 2 ) r f - H 2 -(CH 2 ) r f -SH, (CH 2 ) NH 0 (CH.) -S-lower alkyl,- (CH,) -NH-C ,or-(CH f -C-NH,, x- x - . .. i \ 2 r 2 NH. wherein f , R ] _ 4 f, Pf and rf are as defined above. Rg E is hydrogen, lower alkyl, benzyl, benzhydryl. -^ ώ . i TE SHEE -0- 2 2 f -CH- (CH 2 -OH) 2 -CH 2 - R ] _ 7 is hydrogen, lower alkyl, cycloalkyl, or phenyl. R 18f * 4 ^ s hydrogen, lower alkyl, lower alkoxy, or phenyl or R ] _ 7 and R ] _g taken together are -(CH 2 ) 2 -, -(CH 2 ) 3 -, -CH=CH-, or R 21 and R 22 f are independently selected from hydrogen and lower alkyl. R 23 is lower alkyl. R 24 is hydrogen, lower alkyl. British Specification No. 2095682 published October 6, 1982 discloses N-substituted-N-carboxyalkyl a inocarbonyl alkyl glycine derivatives which are said to SUBSTITUTE S be angiotensin converting enzyme inhibitors and have the formula either (A) R9 and R 9 g are OH, 1-6C alkoxy, 2-6C alkenyloxy, di-(l-6C alkyl)amino- (1-6C) alkoxy, 1-6C hydroxyalkoxy, acylamino- (1-6C)alkoxy, acyloxy- (1-6C)alkoxy, aryloxy, aryloxy-(1- 6C)alkoxy, NH 2 , mono- or di-(l-6C alkyl)amino, hydroxyamino or. aryl- (1-6C)alkylamino; R 1 9 " R 5 9 ' R 7 9 and R8 9 ' are 1-20C alkyl ' , 2-20C alkenyl, 2-20C alkynyl, aryl, aryl-(l-6C) alkyl having 7-12C or heterocyclyl-(1-6C)alkyl having 7-12C; R 9 is cycloalkyl , polycycloalkyl , partly saturated cycloalkyl or polycycloalkyl, cycloalkyl- (1-6C) alkyl having 3-20C, 6-10C ar yl , aryl- (1-6C) alkyl, aryl- ( 2-6C) alkenyl or aryl- ( 2- β C) alkynyl ; or R g and R 3 g together with the C and N atoms to which they are attached or R 3 g and R 5 g together with the N and C atoms to which they are attached form an N-heterocycle containing 3-5C or 2-4C and a S atom; all alkyl, alkenyl and alkynyl are optionally substituted by OH, 1-6C alkoxy, thio(sic), 1-6C alkylthio, NH 2 , mono- or di (1-6C alkyl)amino, halogen or N0 2 ; all 'cycloalkyl' groups (including poly and partially unsaturated) are optionally substituted by halogen, 1-6C hydroxyalkyl, 1-6C alkoxy, amino-(1-6C alkyl)amino, di- (1-6C alkyl)amino, SH, 1-6C alkylthio, N0 2 or CF 3 ; and aryl groups are optionally substituted by OH, 1- 6C alkoxy, NH 2 , mono- or di- (1-6C alkyl) amino, SH, 1-6C alkylthio, 1-6C hydroxyalkyl, 1-6C aminoalkyl, 1-6C thioalkyl, N0 2 , halogen, CF 3 , OCH 2 0, ureido or guanidino; or (B) R g and Rg g are H or 1-6C alkoxy; R χ g and R 2 g are H, 1-6C alkyl, aryl- (1-6C) alkyl having 7-12C or heterocyclyl-(1-6C) alkyl having 6-12C; R 3 g -R 5 g , R 7 g and R 8 g are H or 1-6C alkyl; Rg g is cycloalkyl, polycycloalkyl, partly saturated cycloalkyl or polycycloalkyl, cycloalkyl- (1-6C) alkyl having 3-20C, aryl or aryl-(l-6C) alkyl; and aryl has 6-10C and is optionally substituted by 1-6C alkyl, 2-6C alkenyl, 2-6C alkynyl, OH, 1-6C alkoxy, NH 2 , mono- or di- (1-6C alkyl) amino, SH, 1-6C alkylthio, 1-6C hydroxyalkyl, 1-6C aminoalkyl, 1-6C thioalkyl, N0 2 , halogen, CF 3 , 0CH 2 0, ureido or guanidino. U.S. Patent No. 4,470,972 to Gold et al discloses 7-carboxyalkyl-aminoacyl-l,4-dithia-7- azaspiro[4.4]nonane-8-carboxylie acids which are angiotensin converting enzyme inhibitors and have the formula iCΛ/?o wherein: R n is lower alkyl, benzyl, benzylthio, benzyloxy, phenylthio or phenoxy; Rτ_ is hydroxy or lower alkoxy; R 2 is hydrogen, lower alkyl or amino lower alkyl; and the pharmaceutcally acceptable salts thereof. (i) European Patent Application 0 050 800 published May 5, 1982 discloses carboxyalkyl dipeptides derivatives which are said to be angiotensin converting enzyme inhibitors and have the formula 0 R R 3i R 4i R 51 ? i II I I I I ' ■ -i R 1 -C C—NH— CH— C— N— C— C— R bl I 2 ± » ! 7i R 1 0 R X or a pharmaceutically acceptable salt thereof, wherein R 1 -and R 51 are the same or different and are hydroxy, lower alkoxy, lower alkenyloxy, dilower alkylamino lower alkoxy, acylamino lower alkoxy, acyloxy lower alkoxy, aryloxy, aryllower alkoxy, amino, lower alkylamino, dilower alkylamino, hydroxyamino, aryllower alkylamino, or substituted aryloxy or substituted aryllower alkoxy wherein the substitutent is methyl, halo or methoxy; R is hydrogen, alkyl of from 1 to 10 carbon atoms, substituted lower alkyl wherein the substitutent is hydroxy, lower alkoxy, aryloxy, substituted aryloxy, heteroaryloxy, substituted heteroarylox , amino, lower alkylamino, diloweralkylamino, acylamino, arylamino, substituted arylamino, quanidino, imidazolyl, indolyl, lower alkylthio, arylthio, substituted arylthio, carboxy, carbamoyl, lower alkoxy carbonyl, aryl, substituted aryl, aralkyloxy, substituted aralkyloxy, aralkylthio or substituted aralkylthio, wherein the aryl or heteroaryl portion of said substituted aryloxy, heteroaryloxy, arylamino, arylthio, aryl, aralkyloxy, aralkylthio group SUBSTITUTE £!^τ is substituted with a group selected from halo, lower alkyl, hydroxy, lower alkoxy, amino, aminomethyl, carboxyl, cyano, or sulfamoyl; R x and R' 1 are the same or different and are hydrogen or lower alkyl; R 31 is hydrogen, lower alkyl, phenyl lower alkyl, a inoethylphenyl lower alkyl, hydroxyphenyl lower alkyl, hydroxy lower alkyl, acylamino lower alkyl, amino lower alkyl, dimethylamino lower alkyl, guanidino lower alkyl, imidazolyl lower alkyl, indolyl lower alkyl, or lower alkyl thio lower alkyl; R 41 and R Sl are the same or different and are hydrogen, lower alkyl or Z 1 , or R ~~ and R 51 taken together form a group represented by Q 1 , U 1 , V 1 , Y 1 , D 1 or E 1 , wherein; wherein X 11 and X 2i independent of each other are 0, S or CH 2 , R 81 and R 9i independent of each other are lower alkyl, lower alkenyl, lower alkynyl, cycloalkyl having 3 to 8 carbon atoms, hydroxy lower alkyl, or -(CH 2 ) n i Ar 1 , wherein n 1 is 0, 1, 2 or 3 and Ar 1 is unsubstituted or substituted phenyl, furyl, thienyl or pyridyl, wherein said substituted phenyl, furyl, thienyl or pyridyl groups are substituted with at least one group that is independently selected from C- j _ to C alkyl, lower alkoxy, lower alkylthio, halo, CF 3 and hydroxy, or R 81 and R 9i taken together form a bridge W 1 , wherein 1 is a single bond or a methylene bridge or a- substituted methylene bridge when at least one of X 11 and X is methylene, or 1 is an alkylene or substituted alkylene bridge having 2 or 3 carbon atoms, said substituted methylene bridge or said substituted alkylene bridge having one or two 1 . ?^Λ—. ! * I u If rern substituents selected from lower alkyl, aryl and aryl lower alkyl groups, and p x is 0, 1 or 2; with the proviso that at least one of R 4i and R Sl is Z 1 , with the proviso that if R i is Z 1 and p 1 is 0 then X lx and X 2i must both be methylene, and with the proviso that if X 11 and X 2 are both methylene then R and R 9* ^ must form an alkylene bridge W 1 ; Q 1 is wherein R Sl , R 9x , X 1:L and X 2i are as defined above, p 1 is 0, 1 or 2, q 1 is 0, 1 or 2, with the proviso that the sum of p 1 and q 1 must be 1, 2 or 3, with the proviso that if p 1 is 0 then X 11 and X 2i must be methylene, and with the proviso that if X lx and X 2i are methylene then R 8x and R 9x taken together form a bridge W 1 , wherein 1 is as defined above; v 1 is ■*• " •• "* |, rv^ -». t ___,, —--^- i nUTE SHEET wherein R 8l , R 91 , X 11 and X 21 are as defined above, p x is " 0, 1 or 2 and q 1 is 0, 1 or 2, with the proviso that the sum of p 1 and q 1 is 1, 2 or 3, with the proviso that if together form e; wherein 1 is as defined above (except that 1 may also be a methylene bridge when X 11 and X 21 are. oxygen or sulfur), X 11 and X 2i are as defined above, p 1 is 0, 1 or 2, q 1 is 0, 1 or -2, with the proviso that the sum of p 1 and q 1 is 1 or 2, and with the proviso that if p 1 is 0, X 11 must be CH 2 ; i s wherein G 1 is oxygen, sulfur or CH 2 , a 1 is 2, 3 or 4 and b is 1, 2, 3, 4 or 5, with the proviso that the sum of a 1 and b 1 is 5, 6 or 7 or G 1 is CH 2 , a *1 is 0, 1, 2 or 3, b 1 is 0, 1, 2 or 3 with the proviso that the sum of a 1 and b 1 is 1, 2 or 3, with the proviso that the sum of a 1 and b 1 may be 1, 2 or 3 only if R 11 is lower alkyl substituted with aralkylthio or aralkyloxy; ---. l oTe D x is wherein F 1 is 0 or S, j 1 is 0, 1 or 2 and k 1 is 0, 1 or 2, with the proviso that the sum of j 1 and k 1 must be 1, 2 or 3, and m 1 is 1, 2 or 3 and t 1 is 1, 2 or 3, with the proviso that the sum of m 1 and t 1 must be 2, 3 or 4; E 1 is wherein L 1 is 0 or S, u 1 is 0, 1 or 2 and v 1 is 0, 1 or 2, with the proviso that the sum of u 1 and v 1 must be 1 or 2, and h 1 is 1 or 2 and s 1 is 1 or 2, with the proviso that the sum of h 1 and s 1 must be 2 or 3. (j) European Patent Application 0 037 231 published October 7, 1981 discloses acyl derivatives of octahydro-lH-indole-2-carboxylic acids which are said to be angiotensin converting enzyme inhibitors and have the formula SUBST SI£T * - ** .U.a T /—E- r--. S S * ' J wherein R3 is hydrogen or lower alkyl; ^l is hydrogen, 0 lower alkyl, or benzyl; R 2 1 is hydrogen or R 3 ^-C-wherein R 3 *3 is lower alkyl, heteroaryl containing 4 to 9 carbon atoms and one or two nitrogen, oxygen or sulfur atoms; phenyl, substituted phenyl having 1 or 2 substituents selected from the group consisting of fluorine, chlorine, bromine, lower alkyl or alkoxy; and n is 0 or 1; wherein lower alkyl and lower alkoxy include straight or branched groups containing 1 to 4 carbon atoms, and pharmaceutically acceptable salts of the compounds when R3 is hydrogen and when R 3 ^ is heteroaryl containing 1 or 2 nitrogen atoms, are disclosed. Also disclosed are substituted acyl compounds of octahydro-lH-indole-2- σarboxylic acid having the formula Ar k -(CH 2 ) m k- wherein R 4 k is hydrogen or lower alkyl; R5k is hydrogen, lower alkyl or benzyl; Rg is hydrogen or lower alkyl; Ark Λ i•s phenyl or phenyl substituted with 1 or 2 substituents selected from the group consisting of fluorine, chlorine, bromine, lower alkyl, lower alkoxy, hydroxy or amino; and m is 0 to 3; wherein lower alkyl and lower alkoxy contain 1 to 4 straight or branched carbon atoms; and the pharmaceutically acceptable salts thereof. (L) European Patent Application 0 079 522 published May 25, 1983 discloses N-σarboxymethyl (amino) lysl-proline compounds which are said to be angiotensin converting enzyme inhibitors and have the formula where _ ** *« ' f-i xr-* o u ϋ& R ) L wherein: R- * -- and R ώlJ are independently hydrogen; loweralkyl; aralkyl; or aryl; R 1L is hydrogen; branched or straight chain C l-12 •s-l-ky 1 and alkenyl; C 3 -Cg cycloalkyl and benzofused alkyl; substituted loweralkyl where the substituents are halo, hydroxy loweralkoxy, aryloxy, amino, mono- or diloweralkylamino, acylamino, arylamino, guanidino, mercapto, loweralkylthio, arylthio, carboxy, carboxamido, or loweralkoxycarbonyl; aryl; substituted aryl where the substituents are loweralkyl, loweralkoxy, or halo; arloweralkyl; arloweralkenyl; hete oarloweralkyl; heteroarloweralkenyl; substituted arloweralkyl, substituted arloweralkenyl, substituted heteroarloweralkyl, or substituted heteroarloweralkenyl where the aryl and heteroaryl substituents are halo, dihalo, loweralkyl, hydroxy, loweralkoxy, amino, aminoloweralkyl, acylamino, mono- or diloweralkylamino, carboxyl, haloloweralkyl, nitro, cyano, or sulfonamido, and where the loweralkyl portion of arloewralkyl may be substituted by amino, acylamino, or hydroxyl; SUBSTITUTE Sft where: X L and Y taken together are -CH 2 -CH 2 -; 0 0 0 -CH-S-; -C-CH 2 -; -CH 2 -C Jl-; -C11-0-; -C h -S-; R 5 ^ 0 ; R 4L is hydrogen; loweralkyl; aryl; substituted aryl; R 5L is hydrogen; loweralkyl; aryl or substituted aryl; n L is 1 to 3; 0 II is absent; -CH 2 -; or -C-; Z L is -(CH 2 ) m L , where ra is 0 to 2, provided that m L may not be 0 and W 1 "' may not be absent at the same time; and SUBSTITUTE SHEE S * ->• * .1 _~_- R 6L is hydrogen; loweralkyl; halo; or 0R 4L ; R 2L is (CH 2 ) r ] B L (CH 2 ) S L NR 7L R 15L where r L and s L are independently 0 to 3; B L is absent; -0-; -S-; or -NR 8L ; where R 8L is hydrogen; loweralkyl; alkanoyl; or aroyl; and ,12L -C-R 9L ; -C-NHR 10L. or -C K J where R 9L is loweralkyl; aralkyl; aryl; heteroaryl; or heteroarloweralkyl and these groups substituted by hydroxy, lower alkoxy or halo; carboxyl; carboxamido; nitromethenyl. ' OL is hydrogen; loweralkyl; aryl; or amidino; ,11L hydrogen; loweralkyl; cyano; amidino; aryl; aroyl; loweralkanoyl; -C-NHR 1 L ; - N0 2 ; -S0 2 NH 2 ; or S0 2 R 13L ; R 12L is hydrogen; loweralkyl; halo; aralkyl; amino; cyano; mono- or diloweralkylamino; or OR ; ,13L IS hydrogen; loweralkyl; or aryl; R 15 is hydrogen; lower alkyl; aralkyl; or aryl. constitute a basic heterocycle of 5 or 6 atoms or benzofused analogs thereof and optionally containing 1-3 N atoms, an oxygen, a sulfur, an SJαO . _ a « e £Z. lT-i-««-_ ISA/EP S=0, or an S0 group optionally substituted by amino, lower alkyl amino, diloweralkyl amino, lower alkoxy, or aralkyl groups; R 3L is C3-.8 cycloalkyl and benzofused C 3 _.g cycloalkyl; perhydrobenzofused C 3 _g cycloalkyl; aryl; substituted aryl; heteraryl; substituted heteroaryl; R is hydrogen or loweralkyl; and, a pharmaceutically acceptable salt thereof. (m) U.S. Patent No. 4,256,761 to Suh et al discloses amides which are angiotensin converting enzyme inhibitors and have the general formula C-Y 111 wherein R-J ", R 2 m , R 3 , R 4 m , R 5 m and R 6 m are hydrogen, alkyl, alkenyl, alkynyl, phenyl-alkyl, and cycloalkyl, and may be the same or different, n m is an integer from 0 to 4 inclusive, M ra is alkenyl, alkynyl, cycloalkyl, cycloalkyl- alkyl, polycycloalkyl, polycycloalkyl-alkyl, aryl, aralkyl, heteroaryl, heteroaryl-alkyl, hetero-cycloalkyl, hetero-cycloalkyl-alkyl, fused aryl-cycloalkyl, fused aryl-cycloalkyl- alkyl, fused heteroaryl-cycloalkyl, fused heteroaryl-cycloalkyl-alkyl, alkoxyalkyl, alkylthioalkyl, alkylamino-alkyl, or dialkylaminoalkyl, Y m is hydroxy, alkoxy, amino, or substituted amino, aminoalkanoyl, aryloxy, aminoalkoxy, or hydroxyalkoxy, and — • —m . n rr mm Λ I ( •_. »«•_]» i . ' -- s -~ χ —x i . ___£_• I R 7 m uι is* hydrogen, alkanoyl, carboxyalkanoyl, hydroxyalkanoyl, aminoalkanoyl, cyano, amidino, carbalkoxy, Z m S or Z m SC J wherein Z m is hydrogen, alkyl, hydroxyalkyl, or the radical wherein R- j _ , R 2 m m m -. i Rι m R 6 m n m . M m and m are as described above; and where Y is hydroxy, their non-toxic, pharmaceutically acceptable alkali metal, alkaline earth metal, and amine salts, (n) U.S. Patent No. 4,344,949 to Hoefle et al discloses acyl derivatives of 1, 2,3,4-tetrahydro- isoquinoline-3-carboxylic acid compounds which are angiotensin converting enzyme inhibitors and have the formula SUBSTITUTE SHEE /:VJ> wherein R n is hydrogen, lower alkyl or aralkyl; R-x - is hydrogen, lower alkyl, or benzyl; R 2 n is hydrogen or lower alkyl, and Ar n is phenyl or phenyl substituted with 1 or 2 substituents selected from the group consisting of fluorine, chlorine, bromine, lower alkyl, lower alkoxy, hydroxy or amino; X n and Y n are independently hydrogen, lower alkyl, lower alkoxy, lower alkylthio, lower alkylsulfinyl, lower alkyl- sulfonyl, hydroxy, or X n and Y n together are ethylenediox ; m n is 0 to 3; and the pharmaceu¬ tically acceptable acid salts thereof. (o) European Patent 79022 published May 18, 1983 discloses N-amino acyl-azabicyclooctane carboxylic acid derivatives which have the formula hydroqen atoms at ring positions 1 and 5 are cis to each other and the 3-carboxy group has the endo orientation; R- j _° is H, allyl, vinyl or the side chain of an optionally protected naturally occurrinq α-amino acid; R 2 ° is H, 1-6C alkyl, 2-6C alkenyl or aryl(l-4C alkyl); Y° is H or OH and Z° is H, or Y° and Z° together oxygen; X° is 1-6C alkyl, 2-6C alkenyl, 5-9C cycloalkyl, 6-12C aryl (optionally substituted by one to three 1-4C alkyl or alkoxy, OH, halo, nitro, amino (optionally substituted by one or two 1-4C alkyl), or methylenedioxy) or indol-3-yl). SUBSTITUTE SHEET (p) European Patent 46953 published March 10, 1982 discloses N-amino acyl-indoline and tetrahydro isoguinoline carboxylic acids which are angiotensin coverting enzyme inhibitors and have the formula nP is 0 or 1; A ^ I is a benzene or cyclohexane ring: R- j^ P and R 2 P are each 1-6C alkyl, 2-6C ' alkenyl, 5-7C cycloalkyl, 5-7C cycloalkenyl, 7-12C cycloalkylalkyl, optionally partially hydro- genated 6-10C aryl, 7-14C aralkyl or 5-7 membered monocyclic or 8-10 membered bicyclic heterocyclyl containing 1 or 2 S or 0 and/or 1-4N atoms? all R^ p and R 2 P qroups are optionally substituted, R 3 P is H, 1-6C alkyl, 2-6C alkenyl or 7-14C aralkyl. Preferred R* j _ p is Me and R 2 is phenethyl optionally substituted by halogen. Me or OMe. For purposes of the invention, a preferred ACE inhibitor is 7-[N-(1(S)-carboxy-3-phenylpropyl)-(S)- alanyl]-1,4-dithia-7-azaspiro[4.4]nonane-8(S)-carboxylic acid. Examples of Beta adrenergic blockers are bunolol, atenolol, metoprolol, nadolol, pindolol, propranolol, timolol, labetalol, betaxolol, carteolol and U Tti-; \ * >U=.T f -—E—.SH • ' dilevolol and their isomers. For purposes of the invention a preferred Beta adrenergic blocker is timolol. The preferred pharmaceutical composition of the invention comprises: a) 7-[N-(1(S)-carboxy-3-phenylprop 1)-(S)- alanyl]-l,4-dithia-7-azaspiro[4.4]nonane- 8(S)-carboxylic acid in an amount of from .005 μg to 50 μg; and b) timolol in an amount of from 5μg to 500μg; in combination with an ophthalmologically acceptable carrier for topical use. Angiotensin coverting enzyme (ACE) inhibitors are known in the art. Angiotensin II, a pressor substance, is produced in vivo by the action of anqiotensin convertinq enzyme (ACE) on anqiotensin I. Compounds capable of inhibitinq the action of ACE are clinically useful for controllinq the blood pressure of humans suffering from hypertension. ACE inhibitors are known in the art, and may have a variety of structures. See, for example. An. Rev. Biochem. , 51, 283 (1982) and references cited herein. It can be concluded from test results that any ACE inhibitor will possess the utility described herein; however, for purposes of the present invention the preferred ACE inhibitors are compounds which are capable of inhibitinq the action of ACE by at least 50% at a concentration ~ * f 1 μM or less when tested by the followinq standard method: ACE activity is determined by spectrophoto- metric assay of the product of the hydrolysis of the synthetic substrate, hippuryl histidyl leucine (HHL), as described by Cushman and Cheunq, Biochem. Pharmacol., 20, 1637 (1971). The ACE used is prepared in a manner similar to that of Cheung and Cushman, Biochem. Biophys. Acta., 293, 451 (1973). Incubation- for ACE assays is carried out at 37°C. Each 0.25 ml assay mixture contains the following components: 100 mM of potassium phosphate buffer containing 300 mM sodium chloride, 5 mM HHL and 1.87 U enzyme at pH 8.3 and various concentrations of inhibitors. The enzyme reaction is terminated after 60 minutes by the addition of 0.25 ml of IN HC1. Inhibitors are dissolved in appropriate solvents. Hippuric acid solution for a standard curve is prepared in a similar manner. Each, experiment involves replicate incubations for each condition to be studied. I 5 Q values (the con¬ centration required for the 50% inhibition of ACE activity) are derived from calculated reqression lines. Each experiment utilizes multiple concentrations of inhibitor. Many ACE inhibitors are known in the art and may be prepared by known methods or by variations thereof. The ACE inhibitors of the. invention may exist in isomeric form. The invention contemplates all such isomers both in pure form and admixture, including racemic mixtures. For purposes of the invention, the term beta adrenerqic blocker means a compound which by binding to beta adrenerqic plasma membrane receptors reduces or eliminates sympathetic activity or blocks the effects of exogenously administered catecholamines or adrenergic druqs. See, for example, einer, N. , Drugs That Inhibit Adrenergic Nerves and Block Adrenergic Receptors, in the Pharmaceutical Basis of Therapeutics (ed. A.G. Goodman, L.S. Goodman, A. Gilman), Macmillan Publishing, New York, 1980, 6th ed., pp. 188-197. Examples of preferred beta adrenergic blockers are atenolol (4-[2-hydroxy-3-[(1-methylethyl)amino]- propoxy]benzeneacetamide) , metoprolol (l-[4-(2- SUBSTITUTE SHEE methoxyethyl)phenoxy]-3-[(1-methylethyl)amino]-2- propanol), nadolol (5-[3-[(1,1-dimethylethyl)amino]-2- hydroxypropox ]-1,2,3,4-tetrahydro-2,3-naphthalenediol) , pindolol (l-(lH-indol-4-yloxy)-3-[(1-methylethyl)amino]- 2-propanol) , propranolol (1-[( 1-methylethyl)amino]-3-(1- naphthalenyloxy)-2-propanol), timolol (1-[(1,1- dimethylethyl)amino]-3-[(4-morpholinyl-l,2,5-thiadiazol- 3-yl)oxy]-2-propanol), labetalol (2-hydroxy-5-[1-hydroxy- 2-[(l-methyl-3-phenylpropyl)amino] thyl]-benzamide) , betaxolol,. 1-[4-[2-(Cyclopropylmethoxy)ethyl]-phenoxy]-3- [(methylethyl)amino]-2-propanol, carteolol, 5-[3-[(l,l- dimethylethyl)amino]-2-hydroxypropoxy]-3,4-dihydro-2(1H)- guinolinone, dilevolol, [R-(R,R)]-2-hydroxy-5-[1-hydroxy- 2-[(l-methyl-3-phenylpropyl)amino]ethyl]-benzamide 4- methylbenzenesulfonate salt and bunolol (5-(3-tert- Butylamino-2-hydroxypropoxy)-3,4-dihydro-l(2H)- naphthalinon) . The usefulness of Beta adrenergic blockers for lowering intraocular pressure is known in the art. Thus, the Beta adrenergic blocker timolol, is currently approved by the U.S. Food and Drug Administration for topical intraocular use for the treatment of glaucoma. It is marketed in two dose strengths, i.e., 0.25% and 0.5%. As previously stated, this aqent must be used with caution in a defined patient population because of recognized untoward side effects (see Physicians Desk Reference for Ophthalmoloqy, 11th edition, 1983, p. 126, Medical Economics Co. Inc., Oradell, N.J. 07649). In the followinq paraqraphs certain aspects of the invention are discussed in more detail. The pharmaceutical compositions discussed therein are either compositions comprisinq a beta adrenergic blocker toqether with an ACE inhibitor or are compositions comprising either a beta adrenergic blocker or an ACE inhibitor for combined use of the beta adrenergic blocker SUBSTITUTE SH! ^«/r_3 and the ACE inhibitor. The compositions are for topical ophthalmic use. Preferably the compositions are in the form of solutions, but other known ophthalmic compositions such as for example gels and inserts are contemplated as well. In the discussion the compositions are defined by the amount of active components per dosage unit. Preferred are compositions in the form of solutions for which the dosaqe unit is one drop (50μl). Thus, for example, a. suitable composition is a 0.25% solution (w/v) which is known to contain 2.5 mq of the active component per ml or 125 μg per 50 μl (1 drop). The concentration of the active components in other types of formulations can be established by known methods. It is to be noted that in the treatment of ocular pressure the actual dose needed to give a certain reduction of the intraocular pressure does not only depend on the potency of the active component or combination of components but also on the aqe, size and condition of the individual being treated as well as on the severity of the disease state, especially the maqnitude of the intraocular pressure which is to be reduced. In the followinq discussion the term compound A stands for 7-[N-(1(S)-carboxy-3-phenylpropyl)-(S)- alanyl]-1,4-dithia-7-azaspiro[4.4]nonane-8(S)-carboxylic acid. One aspect of the present invention is to provide pharmaceutical compositions for reduction in intraocular pressure by combined use of a beta adrenerqic blocker and an ACE inhibitor whereby the dosaqe of either one of the components is lower than the dosage used in monotherapy usinq either the beta adrenerqic blocker or the ACE inhibitor alone. The reduction of the IOP provided by this combination therapy is at least as large as obtained by the known monotherapy. The use of SUBSTITUTE SHE diminished doses of the components results in a reduction of severity and frequency of side effects related to * the components. The ' compositions according to this aspect of the invention provide preferably from 50 μg to 250 μg per dosage unit of the beta adrenergic blocker and from 0.025μq to 5μg per dosage unit of the ACE inhibitor, especially from 50μg to 125μg per dosage unit of the beta adrenergic blocker and 0.5μg to l.Oμg per dosage unit of the ACE inhibitor. For example a reduction in intraocular pressure ' equivalent to that obtained by use of the approved clinical dose of timolol may be obtained by use of a lower dose of timolol when such lower dose is combined with an effective amount of compound A. It is anticipated that the use of the diminished dosage of the components especially of timolol will result in a reduction of severity and frequency of related side effects. The composition preferably provides per dosage unit from 50μq to 250μq, especially 50μq timolol and from 0.025μg to 5μg in particular 0.05 to 0.5μq of compound A. Another aspect of the invention is to provide compositions for reduction in intraocular pressure by combined use of a beta adrenerqic blocker and an ACE inhibitor whereby subthreshold doses of both components are provided. Herein the term "subthreshold intraocular pressure reducinq concentration" of ACE inhibitor e.g. 7- [N-(1(S)-carboxy-3-phenylpropyl)-(S)-alanyl]-l,4-dithia- 7-azaspiro[4.4]-nonane-8(S)-carboxylic acid (compound A), means an amount less than the threshold concentration of 0.001% (w/v). Similarly, the term "subthreshold intraocular pressure reducinq concentration" of beta adrenerqic blocker, e.q. timolol, means an amount less than 0.25% (w/v). The threshold concentration of other SUBSTITUTE SHEE ACE inhibitors c is the minimum concentration of the respective ACE inhibitor providing the effect of the 0.001% solution of compound A. The threshold concentration of other beta adrenerqic blockers is the minimum concentration of the respective beta adrenergic blocker providinq the effect of the 0.25% timolol solution. Those skilled in the art will appreciate that the "subthreshold intraocular pressure reducing concentration" will consist of a range of concentrations, and that there will be a lower limit to said concentration below which the present invention will not operate. For purposes of this invention, this lower limit may be considered to be about 5% of the threshold concentration of the particular component. Accordingly for the preferred formulations the term subthreshold dose can be defined as one drop (50μl) of a solution of a concentration between threshold concentration and 5% of the threshold concentration. If compositions other than solutions are utilized the threshold dose can be defined by appropriate terms. The subthreshold intraocular pressure reducinq concentration and dose respectively that is actually utilized, whether for an angiotensin converting enzyme inhibitor or for a particular Beta adrenergic blocker will depend on, inter alia, the potency of each particular material, the combination being administered and the age, size and condition of the patient being treated as well as on the severity of th disease state. The clinical implication of this aspect of the invention is that a combination of the two drugs will result in clinically significant lowering of IOP without the systemic side effects e.g. bradycarida and bronchoconstriction which occur with IOP-lowering effective concentrations of Beta adrenerqic blockers. No SUBSTITUTE SHE significant Beta adrenergic blocker related side effects are projected for the combination. The compositions according to this aspect of the invention provide preferably 5 μg to 125 μg (especially 50 μg to 100 μg) per dosaqe unit of the beta adrenerqic blocker and 0.005 μq to 1 μq (especially 0.05 μq-0.5 μq) per dosaqe unit of the ACE inhibitor. The IOP can be siqnificantly reduced for example by pharmaceutical compositions providing between 6.25 μg and 125 μg (preferably 50 μg) per dosage unit of timolol and between 0.025 μg and 0.5 μg (especially 0.05 μg to 0.5 μg) per dosage unit of compound A. Another aspect of the present invention is to provide pharmaceutical compositions for reducing the intraocular pressure by combined use of a beta adrenergic blocker and an ACE inhibitor whereby the dosage of the one component is the dosage needed to give the maximum lowering of IOP attainable from monotherapy using this component alone and the* dosage of the other component is in the ranqe of subthreshold dosaqes as defined above. In particular the compositions accordinq to this aspect of the invention provide the dosaqe of the beta adrenerqic blocker needed to qive the maximum lowerinq of IOP attainable from monotherapy usinq this beta adrenerqic blocker and a subthreshold dosage of the ACE inhibitor. It is to be noted that the actual dosaqe needed to qive the maximum lowering of IOP attainable in monotherapy depends on the patient and the degree of IOP. The pharmaceutical compositions according to this aspect of the invention give significantly increased lowerinq of IOP pressure. The clinical implication of this is that patients whose elevated IOP (e.q. qlaucoma) is not adequately controlled by maximum recommended w i I _ _ _ £_ \ τ* .ft /^ SHEE -3 concentrations.of standard therapy e.g. timolol, can be treated with the combination of an ACE inhibitor and a beta adrenergic blocker to achieve greater lowering of IOP than that attainable by either treatment alone. Such treatment will result in greater clinical benefit with no increase in side effects. The compositions accordinq to this aspect of the invention preferably provide from 25 μg to 500 μg (preferably 50-250μg) per dosaqe unit of the beta adrenerqic blocker and from 0.005 to 1 μq (preferably 0.025-0.5μq) per dosaqe unit of the ACE inhibitor. For example compositions accordinq to this aspect of the invention provide 50 to 250 μq preferably 250 μq timolol per dosaqe unit and 0.005μq to 0.5μq, preferably 0.005 μg to 0.05 μg of compound A per dosage unit. The intraocular pressure (IOP) lowering effects of the compositions of the invention have been shown by the following procedure wherein timolol as the representative of the beta adrenergic blockers and 7-[N- ( 1(S)-carboxy-3-phenylpropyl)-(S)-alanyl]-1,4-dithia-7- azaspiro[4.4]nonane-8(S)-carboxylic acid (referred to as compound A) as the representative of the anqiotensin converting enzyme inhibitors: Male New Zealand white rabbits weiqhinq 1.3 to 1.8 kq and having a normal IOP (18-23 mmHg) were allowed a 1 week acclimation period prior to starting conditioning and druq studies. Food and water were allowed ad libitum. The rabbits were maintained on a liqht cycle of 12 hr darkness (7 PM to 7 AM) followed by 12 hr of liqht. Animals were conditioned to their handlers and the laboratory settinq and IOP measurements for at least one 4 hr period before being used to study drug effects. SUBSTITUTE SHEE MEASUREMENT OF,IOP Rabbits were restrained in a cloth sack approximately 2 min prior to IOP determinations. The left lower eyelid was retracted forming a pouch and 1 drop of the local anesthetic proparacaine (0.05% w/v) was irrigated over the cornea. The eyelid was then held closed over the eye about 1 min. IOP was usually monitored 1 hr before dosing, at zero time (immediately prior to dosinq), and hourly for the next 4 hr. Only rabbits with IOP's of 18-23 mmHg and showing no sign of ocular irritation were used in drug studies. IOP, in mmHg, was determined in triplicate with a McKaq-Marq applanation tonometer and the averaqe readinq was recorded. The tonometer had previously been calibrated aqainst direct manometric determination of IOP in living eyes of barbiturate-anesthetized rabbits. Internal calibration siqnals of the McKay-Marg unit were checked after every eight readinqs or less. Generally, 16 or more rabbits selected at random were assigned to a placebo or one of several concurrently run drug groups. Prior to drug administration and at each IOP determination, the cornea, iris, and conjunctivae were grossly examined for signs of irritation. Pupil diameter was also examined but detailed measurements were not made. DRUG PREPARATION AND ADMINISTRATION Druq solution were prepared fresh daily no more than 1 hr prior to their administration. One percent solutions (concentrations refer to active material, w/v%) were prepared usinq one of two vehicles (vehicle 1 or PBS, described below). Lower concentrations were prepared by serial dilution with the appropriate vehicle. The pH of these isotonic solutions was 7.4 or 7.0. Fifty μl of test solution was applied with a * * -_' Vi-J m_f i i l i t SHEE i * _.-«.' i_ _ a pipette to the. left eye of conscious rabbits. The method of administration was otherwise identical to that described above for a proparacaine. Rabbits treated with vehicle 1 or compound A solutions were also dosed with 50μl of PBS. Animals treated with timolol were also dosed with either 50μl of vehicle 1 or compound A solutions. The data (mean I SEM) were analyzed using analysis of variance and Dunnett's or Duncan's comparisons and are expressed as peak changes in IOP. Concentrations always refer to active materials and not to their salt form. 3TΪTUTE SHEE mf. /sr.a Compositioπ of Vehicles Used to Dissolve Compounds for Topical Administration Material mg/ml Vehicle l a PBS b Sodium phosphate, diabasic 10. 4 10 . 4 Sodium phosphate, monobasic 2. 4 2 . 4 Chlorobutanol 5. 0 Hydroxypropyl Methylcellulose 5. 0 Purified distilled water qs. ad. 1 .0 ml 0.9% NaCl for injection qs. ad. 1. 0 ml pH 7. 4 7 . 0 a) used for compound A b) Phosphate buffered saline, the vehicle used for timolol' The following results have been obtained: Treatment Peak Change in IOP (mmHg) Vehicle 1 alone -.05±0.5 PBS -0.5±0.5 0.5% Timolol (2) alone -3.0__0.4 (1) 0.00001% compound A alone -1.2__0.4 0.0001% " * -1.7±0.8 0.001% " " " -3.3__0.2 ( 1) 0.5% Timolol plus 0.00001% compound A -e^±o.ed ) ^ ) 0.0001% 0.001% " -e^±o.e^H 3) SUBSTITUTE SHEET 0.1% Timolol plus 0.00001% compound A -4.8±0.4 ( l ( 3 ■ 0.0001% " " -6.3±0.4 (1)(3) 0.001% " " -7.0±0.4 (1)(3) ' ' Statistically significant reduction of IOP vs placebo treatments. ( ' 2 ) ' In this study the concentration of timolol which produced the maximum reduction of IOP in applying timolol as the only IOP reducing agent. ( ' ' Significantly different from response to either timolol or compound A alone. None of the rabbits treated with vehicles, timolol, compound A or timolol in combination with compound A showed any siqns of acute ocular irritation. Pupillary diameters were not affected. This study establishes that combined administration of ACE inhibitors, e.g. compound A and beta adrenergic blockers, e.g. timolol leads to greater acute efficacy in reducinq IOP than can be obtained with either aqent alone. It can be concluded that combined administration of ACE inhibitors, e.g. compound A and beta adrenergic blockers, e.q. timolol is a novel and potentially useful approach for controlling elevated IOP associated with the human simple glaucoma syndrome. The pharmaceutical compositions of the invention (ACE inhibitors and beta adrenerqic blockers) are administered in the form of ophthalmic pharmaceutical compositions adapted for topical administration to the eye; such as solutions, suspensions, ointments and solid inserts. Formulations of the invention may contain the following amounts of each constituent: SϋΣ T.TUTE SHEET ACE inhibitor from 0.00001 to 0.1% (w/v) and especially 0.001 to 0.01% of medicament. As a unit dosage form, an amount of ACE inhibitor from between .005μg to 50 μg, preferably .05μg to 10μg., and especially .5μg to 5μg of the active composition is applied to the human eye. Beta adrenergic blocker from 0.001 to 1% (w/v) and especially 0.01 to 0.5% of medicament. As a unit dosage form, an amount of Beta adrenergic blocker from between .5μg to 500μg and preferably 5μmg to 250μg of the active composition is applied to the human eye. Other concentrations may be employed provided the dose is effective in lowering the intraocular pressure. For example, suitable concentrations of the active components in preferred compositions can be calculated on. the basis of the above detailed discussion of various aspects of the invention. Individual dosage requirements, i.e. the amount of each dose and the frequency of administration will depend on the severity of the disease and the response of the patient. In the preferred method of the invention, it is anticipated that both active ingredients, i.e. ACE inhibitor and beta adrenergic blocker, will be administered simultaneously and be contained in one pharmaceutical dosage form, each component being present in the dosaqe form in its own respective preferred concentration. Where utilized herein, the term "controlling the elevated intraocular pressure associated with glaucoma" means the regulation, attenuation and modulation of increased intraocular tension which is the primary diagnostic symptom of the disease, glaucoma. The term also means that the diminution, in the otherwise elevated intraocular pressure, obtained by the practice of the invention is maintained for a significant period i :"f?' * *•"*■ *-■ Wi °ύ i ITUTE s &H ET of time as for example, between consecutive doses of the composition of the invention. To prepare suitable dosage forms, the active compositions may be conveniently admixed with a non-toxic pharmaceutically acceptable carrier suitable for topical ophthalmolgic administration. Typical of such pharmaceu¬ tically acceptable carriers are, for example, water, mixtures of water and watermiscible solvents such as lower alkanols or vegetable oils, petroleum based jelly, and including also from 0.5 to 5% by weight of hyroxyethyl cellulose, ethyl oleate, carboxymethyl cellulose, polyvinylpyrrolidone, and other water soluble ophthalmologically acceptable non-toxic polymers, for example, cellulose derivatives such as methyl cellulose, alkali metal carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose; acrylates such as polyacrylic acids salts, ethylacrylates; polyacrylamides; natural products such as gelatin, alginates, pectins, tragacanth, karaya, chondrus, agar, acacia; the starch derivatives such as starch acetate, hydroxyethyl starch ethers, hydroxypropyl starch, as well as other synthetic derivatives such as polyvinyl alcohol, polyvinyl pyrrolidone, polyvinyl methyl ether, polyethylene oxide, neutralized carbopol and xanthan gum and mixtures of these polymers. The pharmaceutical preparation may also contain non-toxic auxiliary substances such as emulsifying, preserving, wetting, bodying agents and the like, as for example, polyethylene glycols 200, 300, 400 and 600, carbowaxes 1,000, 1,500, 4,000, 6,000 and 10,000, antibacterial components such as quarternary ammonium compounds; phenylmercuric salts known to have cold sterilizing properties and which are non-injurious in use; thimerosal, methyl and propyl paraben, benzyl alcohol, phenyl ethanol; buffering ingredients such as alkali S metal chloride,* borate, acetate, gluconate buffers; antioxidants such as sodium metabisulfite, butylated hydroxyanisol (BHA) , butylated hydroxytoluene (BHT) and the like; and other conventional ingredients such as sorbitan monolaurate, triethanolamine, oleate, polyoxyethylene sorbitan monopalmitylate, dioctyl alkali metal sulfosuccinate, monothioglycerol, ethylenediamine tetracetic acid and the like. Additionally, suitable ophthalmic vehicles can be used as carrier media for the present purpose including conventional phosphate buffer vehicle systems, isotonic boric acid vehicles, isotonic alkaki chloride vehicles, tris and the like. The pharmaceutical preparation may also be in the form of a solid insert. For example, one may use a solid water soluble polymer as the carrier for the medicament. Inserts that are known in the art that are suitable for this use include those described in British Patent 15611, and in United States Patent 3,993,071; 3,986,510; 3,868,445; and 3,867,510. Solid water insoluble inserts, such as those prepared from ethylene vinyl acetate copolymer, may also be utilized. The compositions of the invention may include additional therapeutic agents in addition to the ACE inhibitor and Beta adrenergic blocker. For example antibiotics, anesthetics as well as other IOP -lowering agents may be present. The pharmaceutical preparation may be prepared according to known methods. SUBSTITUTE SHEET Example: OPHTHALMIC SOLUTION INGREDIENTS WEIGHT % 7-[N- (1(S)-carboxy-3-phenylpropyl) 0.0001-0.001 -S-alanyl]-1,4-dithia-7-azaspiro[4.4] nonane-8(S)-carboxylic acid Timolol 0.1 - 0.5 Benzalkonium Chloride 0.01 Dibasic Sodium Phosphate, Anhydrous 1.04 Monobasic Sodium Phosphate, Monohydrate0.24 Sodium Chloride (q.s. to # adjust to isotonicity) Purified Water q.s. to make 1.0 ml The ingredients are mixed in a suitable mixer. The above descriptions of suitable classes of ACE inhibitors as for use in the present invention were taken from the noted patents and publications or abstracts thereof. Reference should be made to such patents and publications themselves for their full disclosures of such classes and specific compounds within such classes, such patents and publications being incorporated herein by reference for such purposes, and as to any typographical errors or the like which may have occurred in transcription. Also, in describing such suitable ACE inhibitors the superscript letters a-p were included to distinguish among the various classes of compounds and the variable substituent groups thereof. S

Claims: CLAIMS 1. A topical ophthalmologically acceptable composition useful for reducing and/or controlling the elevated intraocular pressure associated with glaucoma which comprises an ophthalmologically acceptable angiotensin converting enzyme inhibitor and an ophthalmologically acceptable beta adrenergic blocker in combination with an ophthalmologically acceptable carrier for topical use. 2. The composition according to claim 1 which comprises an angiotensin converting enzyme inhibitor having the formula (a) wherein R a is hydroxy, NH 2 or lower alkoxy; R*l_ a and R 4 a each is hydrogen, lower alkyl, phenyl or phenyllower alkyl; R 2 a is hydrogen, lower alkyl, phenyl, substituted phenyl wherein the phenyl substituent is halo, lower alkyl or lower alkoxy, phenyl-lower alkyl, diphenyl-lower alkyl, triphenyl-lower alkyl, lower alkylthiomethyl, phenyl-lower alkylthiomethyl, lower alkanoyl-amidomethyl. UTE SHEET R 5 J a -C, R 5 -M a -C- ~ , R 5 a a -NH-C-, R 6 a -S-, or R 7 a ; R 3 a is hydrogen, hydroxy or lower alkyl; Rg a is lower alkyl, phenyl or phenyl-lower alkyl; Rg a is lower alkyl, phenyl, substituted phenyl (wherein the phenyl substituent is halo, lower alkyl or lower alkoxy) , hydroxy-lower alkyl or amino (carboxy)-lower alkyl; M a is 0 or S; m a is 1 to 3; n a and p a each is 0 to 2; (b) having the formula R* j _ is alkyl, aryl, aralkyl, cycloalkyl or cycloalkylalkyl; SUD3TΪTUTE SH Kλ ΞP R 2 is* cycloalkyl , 3-cyclohexenyl or 2-alkyl-3- cyσlohexenyl; R 3 b is alkyl, cycloalkyl or phenyl; R 4 b is H or alkyl; One of R^ and Rg b is H and the other is alkyl- X -, phenyl-X -alkoxy, phenoxy, phenyl, cycloalkyl, alkyl or phenylalkyl; or R 5 b and Rg b together form -X b CH 2 CH 2 X b -, X b is S, SO or S0 2 ; R 7 b is H or CH(R 2 b )OCOR 3 b ; n b is 0 or 1; 'aryl* is phenyl optionally substituted by halo, alkyl, alkoxy, alkylthio, OH, alkanoyl, N0 2 , amino, dialkylamino and/or CF 3 ; alkyl has 1-lOC, cycloalkyl 3-7C, alkoxy 1-8C and alkanoyl 2-9C; (c) having the formula R- and R 2 C are H, 1-7C alkyl, 1-7C haloalkyl, (CH 2 ) m G -D c , 1-7C aminoalkyl or a group of formula: D is cycloalkyl, furyl, thienyl or pyridinyl; A C is (CH 2 ) n -CHR 21 c , NR 22 C -CHR 23 C or 0-CHR 23 °; n" is 0 or 1; q c is 0-7; R 21 c is H, 1-7C alkyl, 1-7C haloalkyl, benzyl or phenethyl; R 2 c is H or 1-7C alkyl; R 23 c is H, 1-7C alkyl, 1-7C haloalkyl or O-t ° is phenyl, 4-hydroxyphenyl, 3,4- dihydroxyphenyl, 3-indolyl, 4-imidazolyl, NH 2 , SH, 1-7C alkylthio, guanidino or CONH 2 ; X c is a group of formula (II) C -(V) C , or NR 4 C - CHR 5 c -COOR 6 c ; (ID (III ) Civ) wher e • ^ 9° and Rg C ar e H and R 7 ° is as def ined below ; s c and R 7 C are H and Rs c is as def ined below; R 7 C and Rg c are H and Rg c is as defined below; Rg C and Rg C are H and -CHR 7 σ - is replaced by -CR 10 c R 10 c. Rg σ is H and R 7 C + Rg C form a double bond; Rg c is H and R 7 C + Rg C complete a fused benzene ring; or Rg G is H and Rg C + R 7 C complete a fused benzene ring; E |C and E" c are H or E »c + E ,,c complete a fused benzene ring; R 7 C is H, 1-7C alkyl, halogen, keto, OH, 2-8C alkanoyl-amino, N 3 , NH 2 , NR 19 C R 20 C , (CH 2 ) ιn c -D c , 0-CONR 15 c R 15 c , 1-7C alkoxy, 1-7C alkylthio, or a group of formula (VI) σ , (VII) c or (VIII) c : (VI) (VII ) ' B |C is a bond or it is 0 or S;Rg C is keto, halogen, 0-CONR 15 c R 15 c , 1-7C alkoxy, 1-7C alkylthio, or a group (VII) c or (VIII) c in which B' σ is 0 or S; . Rg c is keto or a group (VII) c in which B' c is a bond; R 10 c is halogen or Y c ι C . R 11 C ' R I 11 C ' R 12 C and R ' l2 C ar e H or 1"7C alk l , or R ] _ι c is a group of f ormula ( IX) c and the other 3 are H ; - ^-.'p' (IX) c R 13 c is H, 1-4C alkyl, 1-4C alkoxy, 1-4C alkylthio, Cl, Br, F, CF 3 , OH, Ph, PhO, PhS or PhCH 2 ; R 14 σ is H, 1-4C alkyl, 1-4C alkoxy, 1-4C alkylthio, Cl, Br, F, CF 3 or OH; m c is 0-3; p c is 1-3 but it is 2 or 3 only when R; j . 3 C or R 1 c is H, Me, MeO, Cl or F; R 15 c is H or 1-4C alkyl; Y c is 0 or S; R 16° is 1 ~ 4C alkyl or grou (VII) c in which B* c is a bond; or the two R g C groups complete a 5- or 6-membered ring in which the C atoms may each bear 1 or 2 alkyls having 1-4C; R 4 C is H, 1-7C alkyl, cycloalkyl or (CH 2 ) r c Ph; R 5 σ is H, 1-7C alkyl or (CH 2 ) r c -D 1 c ; r c is 1-4; R 3 C and Rg C are H, 1-7C alkyl, PhCH 2 , PhCH or CHR l7 c -0-COR 18 c ; R 17° is H ' 1_7C alkyl or Ph; R 18 ° is H, 1-7C alkyl, 1-7C alkoxy or Ph; or R 17° + R 18 C form (CH 2 ) 2 ( CH 2 ) 3 , -CH=CH or o-phenylene; R 19° ^ s 1 ~ 7C alkyl, benzyl or phenethyl; and R 2Q C is H or chosen as for R ] _9°; (d ) havi ng the formula wherein R and R d are the same or different and are hydroxy, lower alkoxy, lower alkenoxy, dilower alkylamino lower alkoxy (dimethylaminoethoxy), acylamino lower alkoxy (acetylaminoethoxy) , aσyloxy lower alkoxy (pivaloyloxymethoxy) , aryloxy, such as phenoxy, arloweralkoxy, such as benzyloxy, substituted aryloxy or substituted arloweralkoxy wherein the substituent is methyl, halo or methoxy, amino, loweralkylamino, diloweralkylamino, hydroxyamino, arloweralkylamino such as benzylamino; R lc3 is hydrogen, alkyl of from 1 to 20 carbon atoms which include branched and cyclic and unsaturated (such as allyl) alkyl groups, substituted loweralkyl wherein the substituent can be halo, hydroxy, lower alkoxy, aryloxy such as phenoxy, amino, diloweralkylamino, acylamino, such acetamido and benzamido, arylamino, guanidino, imidazolyl, indolyl, mercapto, loweralkylthio, arylthio such as phenylthio, carboxy or carboxamido, car'ooloweralkoxy, aryl such as phenyl or naphthyl, substituted aryl such as phenyl wherein the substituent is lower alkyl, lower alkoxy or halo, arloweralkyl, arloweralkenyl, heteroarlower alkyl or heteroarlower alkenyl such as benzyl, styryl or indolyl ethyl, substituted arloweralkyl, substituted arloweralkenyl, substituted SUBSTITUTE SHEE hetero&rlower alkyl, or substituted heteroarlower alkenyl, wherein the substituent (s) is halo, dihalo, lower alkyl, hydroxy, lower alkoxy, amino, aminomethyl, acylamino (acetylamino or benzoylamino) diloweralkylamino, loweralkylamino, carboxyl, haloloweralkyl, cyano or sulfonamido; arloweralkyl or heteroarloweralkyl substituted on the alkyl portion by amino or acylamino (acetylamino or benzoylamino) ; R d and R 7 -are the same or different and are hydrogen or lower alkyl; R d is hydrogen, lower alkyl, phenyl lower alkyl, aminomethyl phenyl lower alkyl, hydroxy phenyl lower alkyl, hydroxy lower alkyl, acylamino lower alkyl (such as benzoylamino lower alkyl, acetylamino lower alkyl), amino lower alkyl, dimethylamino lower alkyl, halo lower alkyl, guanidino lower alkyl, imidazolyl lower alkyl, indolyl lower alkyl, mercapto lower alkyl, lower alkyl thio lower alkyl; R is hydrogen or lower alkyl; R 5r_ is hydrogen, lower alkyl, phenyl, phenyl lower alkyl, hydroxy phenyl lower alkyl, hydroxy lower alkyl, amino lower alkyl, guanidino lower alkyl, imidazolyl lower alkyl, indolyl lower alkyl, mercapto lower alkyl or lower alkylthio lower alkyl; R d and R 5d may be connected together to form an alkylene bridge of from 2 to 4 carbon atoms, an alkylene bridge of from 2 to 3 carbon atoms and one sulfur atom, an alkylene bridge of from 3 to 4 carbon atoms containing a double bond or an alkylene bridge as above substituted with hydroxy, loweralkoxy, lower alkyl or dilower alkyl;« and the pharmaceutically acceptable salts thereof; (e) having the formula R 4 e is a substituted proline of the formula R Rg e is halogen, keto, azido, -NH- - lower alkyl . SUDSTITUTE SHEET a 1- or 2-naphthyl of the formula (R l ϊ } n β" (CH 2 * ) m e-cyc l oa lk y l . (R io e a 1- or 2-naphthyloxy of the formula • >_- ._- ^_. « _ ! ■• „. » •* -.. ϊ «it_____ -S-lower alkyl . or a 1- or 2-naphthylthio of the formula R 7 e is keto, halogen, a 1- or 2-naphthloxy of the formula -S-lower alkyl . -S- SUBSTITUTE SHEET ISA/EP or a 1- or 2-naphthylthio of the formula Rg e is keto or Rg e is halogen or -Y e -R 13 e . m e is zero, one, two,- or three. Rτ_g e is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, t ifluoromethyl, hydroxy, phenyl, phenoxy, phenylthio, or phenylmethyl. R- * 2 is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, bromo, fluoro, trifluoromethyl, or hydroxy. n e is one, two or three provided that n is more than one only if R ] _n e or R n e **- s hydrogen, methyl, methoxy, chloro, or fluoro. R 12 e -** s hydrogen or lower alkyl of 1 to 4 carbons. Y e is oxygen or sulfur. l3 e is lower alkyl of 1 to 4 carbons. SHE i .χj.--i/ i_r~ or the R ] _ 3 e qroup join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a lower alkyl of 1 to 4 carbons, or a di(lower alkyl of 1 to 4 carbons) substituent. R e and Rg e are independently selected from .hydroxy; lower alkoxy, di(lower alkyl)-amino- lower alkoxy, such as dimethylaminoethoxy, lower alkyl-carbonyl-amino-lower alkoxy, such as acetylaminoethoxy, lower alkyl-carbonyloxy-lower alkoxy, such as pivaloyloxymethoxy. wherein m e , n e and R^ e are as defined above, amino, lower alkyl-amino, di(lower alkyl)-amino hydroxyamino, benzylamino, or phenethylamino. R^ e is hydrogen, lower alkyl. halo substituted lower alkyl, hydroxy substituted lower alkyl, -(CH 2 ) q e -cycloalkyl, -(CH 2 ) q e -carboxy, -(CH 2 ) q e -S-lower alkyl, t /____** - (CH 2 ) q e -guanidinyl, - (CH 2 ) q e -NH 2 -(CH 2 ) q e -N( lower alkyl) 2 -(CH 2 ) alkyl. -(CH 2 ) eNH-C-< θ >-<CH 2 > Ϊ SH ' (CH, -(CH,) e -C-lower alkoxy, or *• ΌΎΈ. SHEET wherein m e , n e , R^g 6 an ^ R li e are as defined above, R e is lower alkyl, cycloalkyl, or n- and q e is an inteqer from 1 to 4. R 2 e is hydrogen or lower alkyl. R e is hydrogen, lower alkyl, halo substituted lower alkyl, hydroxy substituted lower alkyl, -(CH 2 ) q e -NH 2 , -(CH 2 ) q e -N-(lower alkyl) 2 , -(CH 2 ) q e -quanidinyl, -(CH 2 ) q e -SH, -(CH 2 ) q e -S- lower alkyl. - alkyl, or SUBSTITUTE SHEET 10A/EP where n R^g , n and q e are as defined above; (f) having the formula R . Ri— CH — C— CH^- N— CH I —C ?— x ~ ψi C=0 R: X f is an amino or imi no acid of the formula c ? ^ ? S • —.H. 'E~-ET R 7 f is hydrogen, lower alkyl, halogen, keto, hydroxy, 0 II -NH-C- lower alkyl. azido, amino. f t - χ ~-tZr-^-' O _i _• _ ε ST - * -_U rT < χ ~E-s. SHEET a 1- or 2-naphthyl of the formula (R 14 ) p*- <CH 2 ) m-- 0 5' cloalk ' 1 -lower alkyl. P f . a 1- or 2-naphthyloxy of the formula -S-lower alkyl. > p! bHEE or a 1- or 2-naphthylthio of the formula Rg r is keto, halogen, ~ -O-C-N ^ Λ 15 R 15 -O-lower alkyl, a 1- or 2-naphthyloxy of the formula -S-lower alkyl . or a 1- or 2-naphthylthio of the formula SUD3T.TUTE SHE Rg f is * keto or R 10 i* 3 halogen or -Y t -R- [ _g ;t: f f f f Rll , R'II , R 12 anc - R 'l2 are independently selected from hydrogen and lower alkyl or 'n / * F f * F R 12 ant ^ R 'l2 are hydrogen and _]_ is R 1 is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, hydroxy, phenyl, phenoxy, phenylthio, or phenylmethyl. R l4 is hydrogen, lower alkyl of 1 to 4 carbons, lower alkoxy of 1 to 4 carbons, lower alkylthio of 1 to 4 carbons, chloro, bromo, fluoro, trifluoromethyl, or hydroxy. m is zero, one, two, three, or four. p is one, two or three provided that p is more than one only if R l3 f or l is hydrogen, methyl, methoxy, chloro, or fluoro. R- j _5 is hydrogen or lower alkyl of 1 to 4 carbons. Y iis oxygen or sulfur. Rigf is lower alkyl of 1 to 4 carbons. - . U i C r IEET ISA/EP -OL lR f3>p f or the ^g groups join to complete an unsubstituted 5- or 6-membered ring or said ring in which one or more of the carbons has a lower alkyl of 1 to 4 carbons or a di (lower alkyl of 1 to 4 carbons) substituent. R 4 is hydrogen, lower alkyl. "(CH 2>m (CH2,) -cycloalkyl, _> f . - ■ Ά A J EΓJ '- {CE 2 m R 5 f i.s hydrogen, lower alkyl. SHEET - (CH,) f -NH,- (CH,) -SH,-(CH,)^-S-lower alkyl. u 2'r '2'r 2'r r £ is an integer from 1 to 4. R- j _g f is lower alkyl, benzyl, or phenethyl. R 2Q is hydrogen, lower alkyl, benzyl or phenethyl. R ~~ is hydrogen, lower alkyl, cycloalkyl. -(CH 2 ) 2 -NH 2 , -(CH 2 ) 3 -NH 2 , -(CH 2 ) 4 ~NH 2 , -(CH 2 ) 2 -OH, -(CH 2 ) 3 -OH, -(CH 2 ) 4 -OH, -(CH 2 ) 2 -SH, -(CH 2 ) 3 -SH, or -(CH 2 ) 4 -SH. R- | _ is hydrogen , lower alkyl , halo subs ti tuted lower alkyl. .tr-- • • t T> • ~ *• "• . 4r—«x i. χ -»» .- ι_n - . ,_ __ ' ^ '^ ** ** " •'■ T J i j u re SHEE -82- (CH 2> SH , ( C H ) f -OH,-(CH 2 )^-S-lower alkyl, (CH 2 ) f ' f , provided that R^_ is hydrogen only if R is other than hydrogen. R 2 f is t is hydrogen, lower alkyl. bstituted — -=.-... * . lower alkyl ,- (CH 2 )^-cycloalkyl ,-(CH ) _-- - OH ' OH ( CH 2 ) wherein m 1 F, R14f/ PF and rf are as defined above. Rg is hydrogen, lower alkyl, benzyl, benzhydryl, f -CH--CH-CH. ; - (CH.) 2 - N ( C H 3 , ,o_-CH,-fc~) OH OH R-^ 7 r is hydrogen, lower alkyl, cycloalkyl, or phenyl. R 18 s hydrogen, lower alkyl, lower alkoxy, or phenyl or R* ] _ 7 F and taken together are -(CH 2 ) 2 -, -(CH 2 ) 3 -, -CH=CH-, or SUBSTITUTE SHEET A/EP R 21 anc ~ R 22 are independently selected from hydrogen and lower alkyl. R 23^ ~-~~ l° wer alkyl. R 24 i s hydroqen, lower alkyl. (q) havinq the formula either (A) R9 and R g 9 are OH, 1-6C alkoxy, 2-6C alkenyloxy, di-(l-6C alkyl)amino-(1-6C) alkoxy, 1-6C hydroxyalkoxy, acylamino-(l-6C)alkoxy, acyloxy-(l-6C)alkoxy, aryloxy, aryloxy-(1- 6C)alkoxy, NH 2 , mono- or di-(l-6C alkyl)amino, hydroxyamino or aryl-( 1-6C)alkylamino; SUBSTITUTE SHEET R l 9 ~ R f. 9 ' R 7 9 and R 8 9 are i- 200 alkyl, 2-20C alkenyl, 2-20C alkynyl, aryl, aryl-(l-6C) alkyl having 7-12C or heterocyclyl-(1-6C)alkyl having 7-12C; R 6 g is cycloalkyl, polycycloalkyl, partly saturated cycloalkyl or polycycloalkyl, cycloalkyl-(1-6C)alkyl having 3-20C, 6-10C aryl, aryl- (1-6C)alkyl, aryl-(2-6C)alkenyl or aryl-(2- 6C) alkynyl; or R 2 and R 3 together with the C and N atoms to which they are attached or R and Rt j together with the N and C atoms to which they are attached form an N-heterocycle containing 3-5C or 2-4C and a S atom; all alkyl, alkenyl and alkynyl are optionally substituted by OH, 1-6C alkoxy, thio(sic), 1-6C alkylthio, NH 2 , mono- or di (1-6C alkyl)amino, halogen or N0 2 ; all 'cycloalkyl' groups (including poly and partially unsaturated) are optionally substituted by halogen, 1-6C hydroxyalkyl, 1-6C alkoxy, amino- (1-6C alkyl)amino, di-(l-6C alkyl)amino, SH, 1-6C alkylthio, N0 or CF 3 ; and aryl groups are optionally substituted by OH, 1- 6C alkoxy, NH 2 , mono- or di- (1-6C alkyl) amino, SH, 1-6C alkylthio, 1-6C hydroxyalkyl, 1-6C aminoalkyl, 1-6C thioalkyl, N0 2 , halogen, CF 3 , 0CH 2 0, ureido or guanidino; or (B) R g and R 9 g are H or 1-6C alkoxy; R λ q and R 2 g are H, 1-6C alkyl, aryl-(l-6C) alkyl having 7-12C or heterocyclyl-(1-6C) alkyl having 6-12C; R 3 g -R 5 g , R 7 g and Rg are H or 1-6C alkyl; Rg g is* cycloalkyl, polycycloalkyl, partly saturated cycloalkyl or polycycloalkyl, cycloalkyl-(1-6C) alkyl having 3-20C, aryl or aryl-(l-6C) alkyl; and aryl has 6-10C and is optionally substituted by 1-6C alkyl, 2-6C alkenyl, 2-6C alkynyl, OH, 1-6C alkoxy, NH 2 , mono- or di- (1-6C alkyl) amino, SH, 1-6C alkylthio, 1-6C hydroxyalkyl, 1-6C aminoalkyl, 1-6C thioalkyl, N0 2 , halogen, CF 3 , OCH 2 0, ureido or guanidino; (h) having the formula wherein: R is lower alkyl, benzyl, benzylthio, benzyloxy, phenylthio or phenoxy; R- j _ is hydroxy or lower alkoxy; R 2 is hydrogen, lower alkyl or amino_ lower alkyl; and the pharmaceutcally acceptable salts thereof; (i) having the formula R SUBSTITUTE SHEE 1SA/EP or a pharmaceutically acceptable salt thereof, wherein R 1 and R OJ - are the same or different and are hydroxy, lower alkoxy, lower alkenyloxy, dilower alkylamino lower alkoxy, acylamino lower alkoxy, acyloxy lower alkoxy, aryloxy, aryllower alkoxy, amino, lower alkylamino, dilower alkylamino, hydroxyamino, aryllower alkylamino, or substituted aryloxy or substituted aryllower alkoxy wherein the substituent is methyl, halo or methoxy; R 11 is hydrogen, alkyl of from 1 to 10 carbon atoms, substituted lower alkyl wherein the substituent is hydroxy, lower alkoxy, aryloxy, substituted aryloxy, heteroaryloxy, substituted heteroaryloxy, amino, lower alkylamino, diloweralkylamino, acylamino, arylamino, substituted arylamino, guanidino, imidazolyl, indolyl, lower alkylthio, arylthio, substituted arylthio, carboxy, carbamoyl, lower alkoxy carbonyl, aryl, substituted aryl, aralkyloxy, substituted aralkyloxy, aralkylthio or substituted aralkylthio, wherein the aryl or heteroaryl portion of said substituted aryloxy, heteroaryloxy, arylamino, arylthio, aryl, aralkyloxy, aralkylthio group is substituted with a group selected from halo, lower alkyl, hydroxy, lower alkoxy, amino, aminomethyl, carboxyl, cyano, or sulfamoyl; R and R are the same or different and are hydrogen or lower alkyl; R is hydrogen, lower alkyl, phenyl lower alkyl, a inomethylphenyl lower alkyl, hydroxyphenyl lower alkyl, hydroxy lower alkyl, acylamino lower alkyl, amino lower alkyl, dimethylamino lower alkyl, guanidino lower alkyl, imidazolyl lower alkyl, indolyl lower alkyl, or lower alkyl thio lower alkyl; R 41 and R 51 are the same or different and are hydrogen, lower alkyl or Z 1 , or R 1 and R 51 taken together form a group represented by Q 1 , ϋ 1 , v 1 , Y 1 ' oi or E 1 / wherei n? Z 1 is (CH 2 ) p i wherein X 11 and X x independent of each other are 0, S or CH 2 , R 81 and R 91 independent of each other are lower alkyl, lower alkenyl, lower alkynyl, cycloalkyl having 3 to 8 carbon atoms, hydroxy lower alkyl or -(CH 2 ) n Ar 1 , wherein n 1 is 0, 1, 2 or 3 and Ar 1 is unsubstituted or substituted phenyl, furyl, thienyl or pyridyl, wherein said substituted phenyl, furyl, thienyl or pyridyl groups are substituted with at least one group that is independently selected from C- j _ to C 4 alkyl, lower alkoxy, lower alkylthio, halo, CF 3 and hydroxy, or R ox and R -- taken together form a bridge W 1 , wherein W 1 is a single bond or a methylene bridge or a substituted methylene bridge when at least one of X 11 and X x is methylene, or W 1 is an alkylene or substituted alkylene bridge having 2 or 3 carbon atoms, said substituted methylene bridge or said substituted alkylene bridge having one or two substituents selected from lower alkyl, aryl and aryl lower alkyl groups, and is 0, 1 or 2; with the proviso that at least one of R and R is Z 1 , with the proviso that if R 4 is Z 1 and p 1 is 0 then X 11 and X 2i must both be methylene, and with the proviso that if X 11 and X 2i are both methylene then R 8i and R 9i must form an alkylene bridge 1 ; Q 1 is {;.-: * » β iff -~~- wherein R , R , X 11 and X 21 are as defined above, p 1 is 0, 1 or 2, q 1 is 0, 1 or 2, with the proviso that the sum of p 1 and q 1 must be 1, 2 or 3, with the proviso that if p 1 is 0 then X 1* - * and X 21 must be methylene, and with the proviso that if X 1 and X 21 are methylene then R 8i and R 9i taken together form a bridge 1 , wherein 1 is as defined above; V 1 is wherein R 8i , R 9i , x 1 and X 2i are as defined above, p 1 is 0, 1 or 2 and q 1 is 0, 1 or 2, with the proviso that the sum of p 1 and q 1 is 1, 2 or 3, with the proviso that if X 11 and X 2i are CH 2 then R 81 and R 91 taken together form a bridge W 1 , wherein 1 is as defined above; U 1 is wherein 1 is as defined above (except that 1 may also be a methylene bridge when X 11 and X 2i are oxygen or sulfur ) , X 11 and X 2i are as defined above, p 1 is 0, 1 or i is 0, 1 or 2, with the proviso that the sum of pi SUBST II- * T" * . " 'U*T/ * -"E ■ ?*SHEET £ • _.. ■ '. i tr and q 1 is 1 or 2, and with the proviso that if p 1 is 0, wherein G 1 is oxygen, sulfur or CH ' 2 , a 1 is 2, 3 or 4 and b 1 is 1, 2, 3, 4 or 5, with the proviso that the sum of a 1 and b 1 is 5, 6 or 7 or G 1 is CH 2 , a 1 is'0, 1, 2 or 3, b 1 is 0, 1, 2 or 3 with the proviso that the sum of a 1 and b 1 is 1, 2 or 3, with the proviso that the sum of a 1 and b 1 may be 1, 2 or 3 only if R 11 is lower alkyl substituted with aralkylthio or aralkyloxy; D 1 is wherein F 1 is O or S, j 1 is 0, l or 2 and k 1 is 0, 1 or 2, with the proviso that the sum of j 1 and k 1 must be 1, 2 or 3, and m 1 is 1, 2 or 3 and t 1 is 1, 2 or 3, with the proviso that the sum of m 1 and t 1 must be 2, 3 or 4; E 1 is wherein L 1 is 0 or S, u 1 is 0, 1 or 2 and v 1 is 0, 1 or 2, with the proviso that the sum of u 1 and v 1 must be 1 or 2, and h 1 is 1 or 2 and s 1 is 1 or 2, with the proviso that the sum of h 1 and s 1 must be 2 or 3. (j) haying the formula wherein R^ is hydrogen or lower alkyl; R-^ is hydrogen, 0 lower alkyl, or benzyl; R ^ is hydrogen or R ^-C-wherein R ^ is lower alkyl, heteroaryl containing 4 to 9 carbon atoms and one or two nitrogen, oxygen or sulfur atoms; phenyl, substituted phenyl having 1 or 2 substituents selected from the group consisting of fluorine, chlorine, bromine, lower alkyl or alkoxy; and n^ is 0 or 1; wherein lower alkyl and lower alkoxy include straight or branched groups containing 1 to 4 carbon atoms, and pharmaceutically acceptable salts of the compounds when tr* " <-? " ' • • '" * *. • -?"'--•> « -•; 5Υ- .T- f— » » r— x i_. __s RJ is hydrogen and when R 3 -3 is heteroaryl containing 1 or 2 nitrogen atoms, are disclosed; (k) having the formula . Ar -(CH 2 ) m k-CH-NH-C >H 5 COOR g wherein R 4 \e is hydrogen or lower alkyl; R 5 k is hydrogen, lower alkyl or benzyl; R is hydrogen or lower alkyl; Ar Λ is phenyl or phenyl substituted with 1 or 2 substituents selected from the group consisting of fluorine, chlorine, bromine, lower alkyl, lower alkoxy, hydroxy or amino; and m is 0 to 3; wherein lower alkyl and lower alkoxy contain 1 to 4 straight or branched carbon atoms; and the pharmaceutically acceptable salts thereof; (L) having the formula where L R 2 A L L 1 / \ R 1 —CH—NH—C--C0—N--CH (I) L C I OOR L 11 H ' C ' OOR L 11 and R 2L R 3L R 1 ~ - — CH — NH — l C — CO— ! C I OOR L 14 H I ! 14L ( Ia ) L CHR 1 1 I , COOR 11 _ .. . UB8T_TUϊ£ SHEET * . •■ - «■ x*-'- * '-.' ≤ . **31 wherein: R T" and R ώ 2 J T are independently hydrogen; loweralkyl; aralkyl; or aryl; R 1L is hydrogen; branched or straight chain C- j __ 12 alkyl and alkenyl; C 3 -Cg cycloalkyl and benzofused alkyl; substituted loweralkyl where the substituents are halo, hydroxy loweralkoxy, aryloxy, amino, mono- or diloweralkylamino, acylamino, arylamino, guanidino, mercapto, loweralkylthio, arylthio, carboxy, carboxamido, or loweralkoxycarbonyl; aryl; substituted aryl where the substituents are loweralkyl, loweralkoxy, or halo; arloweralkyl; arloweralkenyl; heteroarlowe alkyl; heteroarloweralkenyl; substituted arloweralkyl, substituted arloweralkenyl, substituted heteroarloweralkyl, or substituted heteroarloweralkenyl where the aryl and heteroaryl substituents are halo, dihalo, loweralkyl, hydroxy, loweralkoxy, amino, aminoloweralklyl, acylamino, mono- or diloweralkylamino, carboxyl, haloloweralkyl, nitro, cyano, or sulfonamido, and where the loweralkyl portion of arloweralkyl may be substituted by -amino, acylamino, or hydroxyl; ώϋ ϊ i β _iST - m_ . rrl ■ »S OB. i B where X L and Y L taken together are -CH 2 -CH 2 -; 0 0 Q -CH-S-; -C-CH,-; -CH -C-; -C-0-; -C-S-; 1* L A -C „H 2 - or -CH 2 -C f-R" ,4L is hydrogen, loweralkyl; aryl; substituted aryl; R is hydrogen; loweralkyl; aryl or substituted aryl; n L is 1 to 3; o w L is absent; -CH 2 ; or -C-; Z L is --(CH 2 ) m L , where m L is 0 to 2, provided that πr may not be 0 and W L may not be absent at the same time; and R 6L is hydrogen; loweralkyl; halo; or OR L ; R 2L is- (CH 2 ) B J (CH 2 ) NR 7L R 15L where r L and s L are independently 0 to 3; B L is absent; -0-; -S-; or -NR 8L ; where R 8 L is hydrogen ; lower alkyl ; alkanoyl ; or aroyl ; and where r R_9 5 L i . s loweralkyl ; aralkyl ; aryl ; heteroaryl; or heteroarloweralkyl and these groups substituted by hydroxy , lower alkoxy or halo; σarboxyl ; carboxamido; nitromethenyl. R l θ L is hydrogen ; loweralkyl; aryl ; or amidino ; R 11 J hydrogen; loweralkyl; cyano; amidi no; aryl; aroyl; loweralkanoyl; -C-NHR 13 J or S0 2 R 13 ; ,12 - is hydrogen; loweralkyl; halo; aralkyl; amino; cyano; mono- or diloweralkylamino; or OR 4' L ; , 13 ' I S hydrogen; loweralkyl; or aryl; ,15 " IS hydrogen; lower alkyl; aralkyl; or aryl. constitute a basic heterocycle of 5 or 6 atoms or benzofused analogs thereof and optionally containing 1-3 N atoms, an oxygen, a sulfur, an S=0, or an S0 2 group optionally substituted by amino, lower alkyl amino, diloweralkyl amino, lower alkoxy, or aralkyl groups; is C 3 _g cycloalkyl and benzofused C _g cycloalkyl; perhydrobenzofused C 3 _ cycloalkyl; aryl; substituted aryl; heteroaryl; substituted heteroaryl; .14 IS hydrogen or loweralkyl; and, a pharmaceutically acceptable salt thereof; (m) having the formula wherein R-Lm 1 ", τR_ 2 m U1 , rR, 3 m uι , R _ 4 m uι , -Rc 5 m uι a,n„d -Rgm iU are hydrogen, alkyl, alkenyl, alkynyl, phenyl-alkyl, and cycloalkyl, and may be the same or different, n m is an integer from 0 to 4 inclusive, m is alkenyl, alkynyl, cycloalkyl, cycloalJ-yl- alkyl, polycycloalkyl, polycycloalkyl-alkyl, aryl, aralkyl, heteroaryl, heteroaryl-alkyl, hetero-cycloalkyl, hetero-cycloalkyl-alkyl, fused aryl-cyclpalkyl, fused aryl-cycloalkyl- alkyl, fused heteroaryl-cycloalkyl, fused . heteroaryl-cycloalkyl-alkyl, alkoxyalkyl, alkylthioalkyl, alkylamino-alkyl, or dialkylaminoalkyl, Y m is hydroxy, alkoxy, amino, or substituted amino, aminoalkanoyl, aryloxy, aminoalkoxy, or hydroxyalkoxy, and R 7 is hydrogen, alkanoyl, carboxyalkanoyl, hydroxyalkanoyl, aminoalkanoyl, cyano, amidino, carbalkoxy, Z m S or Z m SC i wherein Z is hydrogen, alkyl, hydroxyalkyl, or the radical c r ϊ < πτυ7E SHEET wherein R ± m , R 2 m , R 3 m , R 4 , R 5 m , Rg m n m , M m and Y m are as described above; and where Y m is hydroxy, their non-toxic, pharmaceutically acceptable alkali metal, alkaline earth metal, and amine salts; or Rl_ n is hydrogen, lower alkyl, or benzyl; R 2 n is hydrogen or lower alkyl, and Ar is phenyl or phenyl substituted with 1 or 2 substituents selected from the group consisting of fluorine, chlorine, bromine, lower alkyl, lower alkoxy, hydroxy or amino; X and Y are independently hydrogen, lower alkyl, lower alkoxy, lower alkylthio, lower alkylsulfinyl, lower alkylsulfonyl, hydroxy, or X and Y together are methylenedioxy; m is 0 to 3; and the pharmaceutically acceptable acid salts thereof; preferably having the formula as defined in paragraph (i) especially being 7-[N-(1 (S)-σarboxy-3-ρhenylpropyl)- (S)-alanyl]- l,4-dithia-7-azaspiro[4.4]nonane-8(S)-carboxylic acid. ff x " * i i . -~J ti ,-χ' (o) <having the formula hydroqen atoms at ring positions 1 and 5 are cis to each other and the 3-carboxy group has the endo orientation; R-ι 0 is H, allyl, vinyl or the side chain of an optionally protected naturally occurring α-amino acid; R 2 ° is H, 1-6C alkyl, 2-6C alkenyl or aryl(l-4C alkyl); Y° is H or OH and Z° is H, or Y° and Z° toqether oxyqen; X° is 1-6C alkyl, 2-6C alkenyl, 5-9C cycloalkyl, 6-12C aryl (optionally substituted by one to three 1-4C alkyl or alkoxy, OH, halo, nitro, amino (optionally substituted by one or two 1-4C alkyl), or methylenedioxy) or indol-3-yl); (p) havinq the formula '"-* • ' ^ * -?€*-*" ■ *<- nzene or cyclohexane ring: R- j _ p and R 2 P are each 1-6C alkyl, 2-6C alkenyl, 5-7C cycloalkyl, 5-7C cycloalkenyl, 7-12C cycloalkylalkyl, optionally partially hydro- genated 6-10C aryl, 7-14C aralkyl or 5-7 membered monocyclic or 8-10 membered bicyclic heterocyclyl containing 1 or 2 S or 0 and/or 1-4N atoms; all R ] _ p and R P groups are optionally substituted. R 3 P is H, 1-βC alkyl, 2-6C alkenyl or 7-14C aralkyl ; 3. The composition according to claim 1 or 2 wherein the beta adrenerqic blocker is atenolol, metoprolol, nadolol, pindolol, propranolol, timolol, labetalol, betaxolol, carteolol, dilevolol or butonolol, preferably timolol. 4. The composition accordinq to any one of claims 1 to 3 wherein for an amount of from 0.005ug to 50yg of the ACE inhibitor there is present an amount of from 5μg to 500μg of the beta adrenerqic blocker, whereby the following proportions are preferred (a) 50μg to 250μg (especially 50μg to 125μg) of the beta adrenergic blocker to 0.025μg to 5μq (especially 0.05μg to 0.5μg) of the ACE inhibitor ; (b) 5μg to 125μg (especially 50μq) of the beta adrenerqic blocker to 0.005μq to lug (especially 0.05μq to 0.5μq) of the ACE inhibitor; and SUBSTITUTE SHEET (c) 25μg * to 500μg (especially 50μg to 250μg) of the beta adrenergic blocker to 0.005μg to lμg (especially 0.005μg to 0.05μg) of the ACE inhibitor. 5. A kit comprisinq in separate containers pharmaceutical compositions for combination therapy to reduce and/or control the elevated intraocular pressure associated with glaucoma in mammals which comprises in the one container a topical ophthalmological pharmaceutical composition comprising an ophthalmologically acceptable angiotensin converting enzyme inhibitor and in the second container, a topical ophthalmological pharmaceutical composition comprising an ophthalmoloqically acceptable beta adrenerqic blocker. 6. * The kit according to claim 5 wherein the -ACE inhibitor is an ACE inhibitor as defined in claim 2 and . as disclosed in (a) U.S. Patent No. 4,105,776, (b) EP Publ. No. 97050, (c) EP Publ. No. 83172, (d) EP Publ. No. 12 401, (e) US Pat. No. 4,462,943, (f) US Pat. No. 4,470,973, (q) British Specific. No. 2095682, (h) US Pat. No. 4,470,972, (i) EP Publ. No. 50 800, (j) and (k) EP Publ. No. 37 231, (L) EP Publ. No. 79 522, (m) US Pat. No. 4,256,761, (n) US Pat. No. 4,344,949, (o) EP Publ. No. 79022, or (p) EP Publ. 46953, preferably an ACE inhibitor disclosed in (i) EP Publication No. 50800, especially [7- N-( 1(S )-ca boxy-3-phenylpropyl )-(S )-alanyl] -1,4-dithia-7- azas iro [4.4] nonane-8 (S)-carboxylic acid. 7. The kit according to claim 5 or 6 wherein the beta adrenergic blocker is atenolol, metoprolol, nadolol, pindolol, propranolol, timolol, labetalol, betaxolol, carteolol, dilevolol or bunolol, preferably timolol. SUBSTITUTE S F 8. The kit according to any one of claims 5 to 7 wherein for an amount of from 0.005μg to 50μg of the ACE inhibitor there is present an amount of from 5μg to 500μg of the beta adrenergic blocker, whereby the followinq proportions are preferred (a) 50μq to 250μq (especially 50μq to 125μg) of the beta adrenerqic blocker to 0.025μg to 5μq (especially 0.05μq to 0.5μq) of the ACE inhibitor ; (b) 5μq to 125μq (especially 50μq) of the beta adrenergic blocker to 0.005μg to lμg (especially 0.05μq to 0.5μg) of the ACE inhibitor; and (c) 25μg to 500μq (especially 50μq to 250μg) of the beta adrenergic blocker to O.OQSμg to lμq (especially 0.005μq to 0.05μg) of the ACE inhibitor. 9. A kit comprising (a) a topical ophthalmological pharmaceutical composition comprisinq an ophthalmologically acceptable anqiotensin converting enzyme inhibitor and (b) a topical ophthalmoloqical pharmaceutical composition comprisinq an ophthalmoloqically acceptable beta adrenerqic blocker, toqether with (c) instructions for using the compositions for combination therapy to reduce and/or control the elevated intraocular pressure associated with qlaucoma in mammals. 10. The kit according to claim 9 wherein the ACE inhibitor is an ACE inhibitor as defined in claim 2 and as disclosed in SUBSTITUTE SHEET (a) U.S. Patent No. 4,105,776, (b) EP Publ. No. 97050, (c) EP Publ. No. 83172, (d) EP Publ. No. 12 401, (e) US Pat. No. 4,462,943, (f) US Pat. No. 4,470,973, (g) British Specific. No. 2095682, (h) US Pat. No. 4,470,972, (i) EP Publ. No. 50 800, (j) and (k) EP Publ. No. 37 231, (L) EP Publ. No. 79 522, (m) US Pat. No. 4,256,761, (n) US Pat. No. 4,344,949, (o) EP Publ. No. 79022, or (p) EP Publ. 46953, preferably an ACE inhibitor disclosed in (i) EP Publication No. 50800, especially [7- N-( l(S)-carboxy-3-phenylpropyl)-(S)-alanyl]-1, 4-dithia-7- azaspiro [4.4] nonane-8 (S)-carboxylic acid. 11. The kit according to claim 9 or 10 wherein the beta adrenergic blocker is atenolol, etoprolol, nadolol, pindolol, propranolol, timolol, labetalol, betaxolol, carteolol, dilevolol or bunolol, preferably timolol. 12. The kit accordinq to any one of claims 9 to 11 wherein for an amount of from 0.005μg to 50μg of the ACE inhibitor there is present an amount of from 5μg to 500μg of the beta adrenergic blocker, whereby the following proportions are preferred (a) 50μg to 250μg (especially 50μg to 125μg) of the beta adrenerqic blocker to 0.025yg to 5μg (especially _0..0_5_μg_ to _0.5μg) of the ACE inhibitor ; (b) 5μg to 125μg (especially 50μg) of the beta adrenerqic blocker to 0.005μq to lμg (especially 0.05μg to 0.5μq) of the ACE inhibitor; and (c) 25μg to 500μg (especially 50μq to 250μq) of the beta adrenerqic blocker to 0.005μq to lμq (especially 0.005μq to 0.05μq) of the ACE inhibitor. 13. The use of an ophthalmologically acceptable ACE inhibitor and an ophthalmologically acceptable beta adrenerqic blocker for the preparation of medicaments for combination therapy to reduce and/or control the elevated intraocular pressure associated with qlaucoma. 14. The use according to claim 13 wherein the ACE inhibitor used is an ACE inhibitor as defined in claim 2 and as disclosed in (a) U.S. Patent No. 4,105,776, (b) EP Publ. No. 97050, (c) EP Publ. No. 83172, (d) EP Publ. No. 12 401, (e) US Pat. No. 4,462,943, (f) US Pat. No. 4,470,973, (g) British Specific. No. 2095682, (h) US Pat. No. 4,470,972, (i) EP Publ. No. 50 800, (j) and (k) EP Publ. No. 37 231, (L) EP Publ. No. 79 522, (m) US Pat. No. 4,256,761, (n) US Pat. No. 4,344,949, (o) EP Publ. No. 79022, or (p) EP Publ. 46953, preferably an ACE inhibitor disclosed in (i) EP Publication No. 50800, especially [7- N-( 1(S)-ca boxy-3-phenylpropyl)-(S)-alanyl]-1,4-dithia-7- azaspiro[4.4]nonane-8(S)-carboxylic acid. 15. The use accordinq to claim 13 or 14 wherein the beta adrenerqic blocker is atenolol, metoprolol, nadolol, pindolol, propranolol, timolol, labetalol, betaxolol, carteolol, dilevolol or bunolol, preferably timolol. 16. The use accordinq to any one of claims 13 to 15 wherein for an amount of from 0.005μq to 50μq of the ACE inhibitor there is used an amount of from 5μq to 500μq of the beta adrenerqic blocker, whereby the following proportions are preferred ^ (a) <50μq to 250μg (especially 50μq to 125μq) of the beta adrenerqic blocker to 0.025μg to 5μg (especially 0.05μq to 0.5μq) of the ACE inhibitor; (b) 5μq to 125yq (especially 50μg) of the beta adrenergic blocker to 0.005μg to lμg (especially 0.05μg to 0.5μg) of the ACE inhibitor; and (c) 25μg to 500μg (especially 50μg to 250μg) of the beta adrenerqic blocker to 0.005μq to Iμq (especially 0.005μq to 0.05μq) of the ACE inhibitor. 17. Process for the preparation of a topical ophthalmoloqically acceptable composition useful for reducinq and/or controllinq the elevated intraocular pressure associated with qlaucoma which comprises mixing an ophthalmoloqically acceptable angiotensin converting enzyme inhibitor and an ophthalmoloqically acceptable beta adrenerqic blocker with an ophthalmologically acceptable carrier for topical use. 18. The process accordinq to claim 17 wherein the ACE inhibitor is an ACE inhibitor as defined in claim 2 and as disclosed in ' (a) U.S. Patent No. 4,105,776, (b) EP Publ. No. 97050, (c) EP Publ. No. 83172, (d) EP Publ. No. 12 401, (e) US Pat. No. 4,462,943, (f) US Pat. No. 4,470,973, (q) British Specific. No. 2095682, (h) US Pat. No. 4,470,972, (i) EP Publ. No. 50 800, (j) and (k) EP Publ. No. 37 231, (L) EP Publ. No. 79 522, ( ) US Pat. No. 4,256,761, (n) US Pat. No. 4,344,949, (o) EP Publ. No. 79022, or (p) EP Publ. 46953, preferably an ACE inhibitor disclosed in (i) EP Publication No. 50800, especially [7- SUSSTfTUTE SHEET N-(l(S)-carboxy-3-phenylpropyl)-(S)-alanyl]-l,4-dithia-7- azaspiro [4.4] nonane-8(S)-carboxylic acid. 19. The process according to claim 17 or 18 wherein the beta adrenergic blocker is atenolol, metoprolol, nadolol, pindolol, propranolol, timolol, labetalol, betaxolol, carteolol, dilevolol or bunolol, preferably timolol. 20. The process accordinq to any one of claims 17 to 19 wherein for an amount of from 0.005μq to 50μg of the ACE inhibitor there is present an amount of from 5μg to 500μg of the beta adrenerqic blocker, whereby the followinq proportions are preferred (a) 50μq to 250μg (especially 50μg to 125μg) of the beta adrenerqic blocker to 0.025μg to 5μq (especially 0.05μq to 0.5μg) of the ACE inhibitor ; (b) 5μg to 125μg (especially 50μg) of the beta adrenerqic blocker to 0.005μg to lug (especially 0.05μq to 0.5uq) of the ACE inhibitor; and (c) 25uq to 500uq (especially 50μq to 250uq) of the beta adrenerqic blocker to 0.005ug to lug (especially 0.005uq to 0.05uq) of the ACE inhibitor. SUBSTITUTE SHEET

Inventors: BARNETT ALLEN, WATKINS ROBERT WAYNE, BARNETT, ALLEN, WATKINS, ROBERT, WAYNE
Applicants: SCHERING CORP, SCHERING CORPORATION
Application year: 1986
Main classification: A61K45
IPC codes: A61K38, A61K45