PHOSPHONYLMETHOXYALKYL ADENINES FOR THE TREATMENT OF VIRUS DISEASES

Abstract: The (S) and (RS) forms of certain phosphonylmeth­ oxyalkyl adenines and their salts have an antiviral effect against several DNA viruses and can be used for the treat­ ment of virus diseases in human and veterinary medicine.

Description: This invention relates to a novel therapeutical agent for virus diseases and to its preparation. It is known that certain substituted alkyl derivatives of adenine exhibit a marked antiviral activity. Such adenine derivatives are: 9-(S)-(2,3-dihydroxypropyl)adenine as disclosed in U.S. - A - 4.230.708, D-eritadenine as disclosed by Holy et al. in Coll. Czech. Chem. Comm., 47 , 1392-1407 (1982) and 3-(adenin-9-yl)-2-hydroxypropanoic acid esters as disclosed in U.S. - A - 4.605.658. Although these adenine derivatives have been indicated as broad-spectrum antiviral agents, it appears that their antiviral activity is directed predominantly against RNA-viruses such as measles, parainfluenza and vesicular stomatitis virus and further only against a single DNA-virus, viz. vaccinia virus. Their activity against the majority of DNA-viruses such as herpes simplex and herpes zoster, however, is rather minimal or totally absent. Therefore, there is a need for antiviral adenine derivatives having their antiviral activity directed predominantly against DNA-viruses and a primary object of the invention is to provide adenine derivatives and therapeutic compositions having such activity. In accordance with the invention, it has now been found that certain phosphonylmethoxyalkyl adenines exhibit a marked antiviral activity against several DNA-viruses such as vaccinia and herpes viruses, while being ineffective against the majority of RNA-viruses and having a low toxicity for host cells. This is surprising since antiviral activities are shown only by a limited number of adenine derivatives and since it could not be expected that the introduction of a phosphonylmethoxy group would induce such a drastic shift in antiviral activity. The phosphonylmethoxyalkyladenines of the present invention can be represented by the following general formula (I) wherein R¹ is methylene, -CH(OH)-CH₂- or - H-CH₂OH and R² is a hydroxyl group and in some cases R¹ and R² are linked with each other to form a cyclic ester group. They can be used in the form of free acids or in the form of salts such as alkalimetal, ammonium or amine salts. Most of the compounds of formula (I), except those wherein R¹ is methylene, will have an asymmetric carbon atom and will exist in more than one enantiomeric form. It should be noted that in that case, only the (S)-form of the compounds and also the racemic or (RS) form will show sufficient antiviral activity. The best antiviral activity among the compounds of formula (I) is shown by ( S )-9-(3-hydroxy-2-phosphonylmethoxypropyl)-adenine. It should be noted that most of the derivatives of formula (I) were already disclosed in GB-A-2 134 907 where the intended use is for sterilizing insects. Therefore, the compounds as such and their chemical syntheses are not a part of the present invention. Further, antivirally active phosphonyl-alkyloxymethyl-adenines were suggested on page 9 of WO-A-84/04748 (EP-A-0 145 739) without, however, being exemplified therein. Moreover, a few antivirally active phosphonyl derivatives of guanine were disclosed in the literature. Thus, an in vitro antiherpes activity of 9-(3-phosphono-1-propyloxymethyl)-guanine and of 9-(3-ethylphosphono-1-propyloxymethyl)-guanine was disclosed in the examples of WO-A-84/04748 and by Reist et al. in "Proceedings 78th Symp. Med. Chem. 1 , pp. 241-242 (1985). An antivirally active phosphonyl derivative of guanidine indicated as GppCH₂p was disclosed by L. Carrosco, Nature 272 , pp. 694-696 (1978). The derivatives of formula (I) can be prepared in genealong at least two routes, viz. (A) condensation of a 9-(hydroxyalkyl)-adenine with chloromethanephosphonylchloride followed by alkaline hydrolysis (GB-A-2 134 907) and (B) condensation of a 9-(hydroxyalkyl)-adenine with p-toluenesulphonyloxymethylenephosphoric acid diester followed by removal of the ester groups with trimethyliodosilane. Racemic mixtures can be separated into their components either before or after the preparation and if the product is a free acid, it can be converted to a salt by reaction with an alkali metal, ammonia or with amines. As stated before, the derivatives of formula (I) have a low toxicity for host cells. The cytotoxic effect of these derivatives on tissue culture cells is only observed at concentrations higher than 100 or 200 or 400 µg/ml, i.e. at concentrations which are substantially higher than the active virostatic concentrations (compare Table 2 below). Further, the derivatives of formula (I) are ineffective in tissue culture cells infected with the majority of RNA-viruses such as reoviruses, rhabdoviruses, paramyxoviruses, enteroviruses. Some of the derivatives are very active, however, against Moloney murine sarcoma virus, a retrovirus which induces sarcomatous tumors in mice (compare Table 3) and, therefore, these derivatives may be expected to be effective against retroviruses in general. The principal target of the compounds of formula (I) is formed by DNA-viruses like vaccinia viruses and herpes simplex virus. All such compounds inhibit the replication of several strains of herpes simplex viruses of types 1 and 2 (compare Table 4). When compared to known compounds used in therapeutic compositions for the treatment of diseases caused by herpes viruses, such as acyclovir, ( E )-5-(2-bromovinyl)-2'-deoxyuridine, and 5-iodo-2'-deoxyuridine, the compounds of formula (I) are less potent; however, they lack the disadvantages of such compounds, viz. the low solubility of acyclovir, the mutagenic, teratogenic, embryotoxic and carcinogenic potential of 5-iodo-2'-deoxyuridine. An important and substantial feature of the compounds of formula (I) is their effect on those herpes virus strains which are deficient in thymidine kinase inducing activity (TK⁻-strains). Thymidine kinase is essential for the activity of acyclovir and ( E )-5-(2-bromovinyl)-2'-deoxyuridine, which are thus inactive against TK⁻herpes viruses (Prusoff et al., pages 1-27 in: "Targets for the Design of Antiviral Agents", Eds. De Clercq and Walker, Plenum Press, New York and London, 1984). To the contrary, the compounds of formula (I) have a distinct activity against TK⁻ herpes viruses (compare table 4). The compounds of formula (I) can therefore be used in the therapy of diseases caused by TK⁻herpes virus strains and, furthermore, they may be useful in combination with acyclovir and other known compounds. The antiviral activity of the derivatives of formula (I) is entirely novel and unexpected. It is known that phosphoric acid esters of antivirally active nucleoside analogues such as adenine arabinoside, 5-ethyl-uracil arabinoside, acyclovir, 5-iodo-2'-deoxyuridine and ( E )-5-2-bromovinyl)-2'deoxyuridine, are also effective as antiviral agents. These esters merely act, however, as prodrugs and even before they have the opportunity to enter the cells, they are hydrolyzed to release the free nucleoside analogues. The compounds of formula (I) are equally polar as the above phosphoric acid esters. They can penetrate, however, as such into the cells and hence produce the desired biological effects without any hydrolysis. Therapeutic compositions containing compounds of formula (I) as an active ingredient for treating virus diseases in human and veterinary practice may take the form of powders, suspensions, solutions, sprays, emulsions, unguents or creams and may be used for local application (lesions of skin, mucosa, eye, etc.) for intranasal, rectal, vaginal and also for oral or parenteral (intravenous, intradermal, intramuscular, intrathecal etc.) administration. Such compositions may be prepared by combining (e.g. mixing, dissolving etc.) the active compound of formula (I) in the form of a free acid or a salt with pharmaceutically acceptable excipients of neutral character (such as aqueous or non-aqueous solvents, stabilizers, emulsifiers, detergents, additives), and further, if necessary, with dyes and aromatizers. The concentration of the active ingredient in the therapeutic composition may vary widely between 0.1 percent and 100 percent, dependent on the character of the disease and the mode of administration. Further, the dose of the active ingredient to be administered may vary between 0.1 mg and 100 mg per kg of body weight. The antiviral activity of the compounds of formula (I) is documented by the following examples which in no way limit their use. The following abbreviations are used in the examples and tables: MCC - minimum compound concentration which brings about a microscopically detectable change in cell morphofology; MIC₅₀ - minimum compound concentration which will cause a 50% inhibition of the cytopathic effect of the virus; ID₅₀ - concentration of compound which causes a 50% decrease of incorporation of precursors labeled with tritium (³H); dThd - 2'-deoxythymidine; Urd - uridine; Leu - L-leucine. Cell lines: PRK - primary tissue culture rabbit kidney cells; Vero B and Vero Flow - continuous lines of African green monkey kidney cells; HeLa - continuous line of human cervical carcinoma cells; MO - continuous murine embryonic cell line. Designation of compounds of formula (I): No. 1: ( RS )-9-(2(3)hydroxy-3(2)-phosphonylmethoxypropyl)-adenine. No. 2: ( RS )-9-(2-hydroxy-3-phosphonylmethoxypropyl)-adenine. No. 3: ( S )-9-(2-hydroxy-3-phosphonylmethoxypropyl)-adenine. No. 4: ( S ) -9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine. No. 5: 2',3'-cyclic O-phosphonylymethyl ether of ( RS )-9-(2,3-dihydroxypropyl)adenine. No. 6: 9-(2-phosphonylmethoxyethyl)adenine. Designation of the other compounds: ( S )- DHPA, 9-(S)-(2,3-dihydroxypropyl)adenine; ACV, aclycovir; BVDU, ( E )-5-(2-bromovinyl)-2'-deoxyuridine; IDU, 5-iodo-2'-deoxyuridine. Compounds nos. 1-6 have been prepared as follows: compound no. 1 (a mixture of isomers) was prepared by reaction of ( RS )-9-(2,3-dihydroxypropyl)adenine with chloromethylphosphonyl dichloride followed by an alkaline treatment. Compound no. 2 was isolated from compound no. 1 by chromatographic separation. Compounds nos. 3 and 4 were prepared by reaction of ( S )-9-(2,3-dihydroxypropyl)adenine with chloromethylphosphonyl dichloride followed by an alkaline treatment and by chromatographic separation on an ion exchange resin. Compound no. 5, a mixture of isomers, was synthesized by cyclization of compound no. 1 with N,N-dicyclocarbodiimide. Compound no. 6 was prepared by reaction of 9-(2-hydroxyethyl)adenine with diethyl p-toluene sulfonyloxymethane phosphonate followed by cleavage of the ester groups. Some physical data obtained by paper chromatography and electrophoresis are given in Table 1. Compound R f E up k No. 1 0.10 0.82 2.37 + 2.63 No. 2 0.10 0.82 2.63 No. 3 0.10 0.82 2.63 No. 4 0.10 0.82 2.37 No. 5 0.38 0.47 3.00 + 3.45 No. 6 0.15 0.82 2.31 The R f values were obtained by paper chromatography in 2-propanol-conc. aqueous ammonia-water (7:1:2, vol/vol). The values of E up were obtained by paper electrophoresis (20V/cm) at pH 7.5 and show the mobility related to uridine 3-phosphate. The k-values were obtained by HPLC on Separon six RPS (5/µm) (4 x 200 mm, 0.7 ml/min.) in 5 vol. % methanol in 0.05 mol/l triethylammonium hydrogen carbonate at pH 7.5. The detection was at 254 nm. k= (t r -t o )/t o when t r is the retention time and t o is the hold-up time. Example 1 Determination of cytotoxicity and antimetabolic activity of compounds of formula (I) in tissue cultures. Primary rabbit kidney cell cultures were incubated in Petri dishes with solutions of compounds of formula (I) at increasing concentrations (1-400 µg/ml) in Eagle's minimum essential medium for 72 h; the morphological changes of the cells in the individual dishes were evaluated microscopically. The minimum cytotoxic concentration (MCC) shown in table 2 is the lowest concentration of the compound at which morphological changes of the cells were observed under the experimental conditions used. For the determination of the antimetabolic effects of the compounds of formula (I), the primary rabbit kidney cell cultures in Eagle's culture medium were incubated in the presence of increasing concentrations of the compounds and a fixed amount of radiolabeled precursor (37.10³Bq (1µCi) per dish, (³H-methyl)dThd 1110.10⁹ Bq/mmol (30 Ci/mmol), (³H-1¹,2¹)dUrd 999.10⁹ Bq/mmol (27 Ci/mmol), (³H-5)Urd 1110.10⁹ Bq/mmol (30 Ci/mmol), (³H-4,5)Leu 1739.10⁹ Bq/mmol (47 Ci/mmol). The concentration of the labeled precursor was determined as described earlier (De Clercq et al, Biochem. Pharmac. 24 , 523 (1975)). The ID₅₀ values of the compounds of formula (I) which cause a 50 % decrease of the incorporation of the precursor compared to controls not containing the compound are listed in table 2. It is obvious from these values that none of the compounds of formula (I) shows a marked cytotoxicity and that only compounds nos. 4 and 6 specifically affect the incorporation of 2'-deoxyuridine into cells at a concentration of about 100 µg/ml. Example 2 Determination of antiviral effect of compounds of formula (I) in tissue cultures . The cell cultures were infected with 100-fold the virus dose required for infection of half of the cells (100 CCID₅₀). 1 hour after infection, the cells were further incubated in the presence of Eagle's medium containing increasing concentrations of the compound of formula (I). Using a series of compounds assayed at different concentrations, the minimum compound concentration necessary to produce a 50 % inhibition of the cytophatic effect of the virus (MIC₅₀) was determined by the method of Rosenthal and Schechmaister ("Tissue Culture", Pergamon Press, New York, p. 510, 1973). From the data presented in Table 3, it is clear that compounds of formula (I) efficiently inhibit vaccinia virus, herpes simplex virus type 1 and 2 and Moloney sarcoma virus. Example 3 Determination of the antiviral effect of compounds of formula (I) on herpes viruses . The experiment was carried out as described under Example 2 with primary rabbit kidney (PRK) cells infected by 100 CCID₅₀ of various strains of herpes simplex virus of type 1 and 2, and with human embryonic lung (HEL) cells infected with 20 PFU (plaque-formation units) of various strains of varicella zoster virus and cytomegalovirus. The evaluation of the effect of compounds of formula (I) is given in Tables 4 and 5. These data show that all the compounds were active against the cited viruses, including the TK⁻-mutants. In this respect, compound no. 4 was the most effective one. Example 4 Effects of compounds of formula (I) on the multiplication (yield) of herpes simplex virus type 1 and vaccinia virus. The primary rabbit kidney cells cultures (PRK) were infected with a virus dose of 10 4.5 PFU/0.5ml (PFU being one unit of plaque formation) of herpes simplex virus type 1 (strain KOS) or vaccinia virus, as described under example 2. The cells were subsequently exposed to compound (I) (at 100 µg/ml) in Eagle's culture medium. The virus yield was determined after 1, 24, 48 and 72 h by determining the number of plaques in PRK cells. The data listed in table 6 show that all compounds tested (nos. 1,2,4,5,6) decreased the yield of herpes simplex virus type 1 (KOS) and vaccinia virus by 10,000 to 100,000-fold as determined at either 1, 2 or 3 days after virus infection. Cytotoxic and antimetabolic effects of compounds of formula I on PRK cells Compound MCC (µg/ml) ID₅₀ (µg/ml) dThd dUrd Urd Leu No. 1 ≧100 250 230 >400 >400 No. 2 >400 280 >400 >400 >400 No. 3 ≧200 >400 >400 >400 >400 No. 4 ≧200 297 74 >400 >400 No. 5 >400 ≧390 ≧360 >400 >400 No. 6 ≧200 242 114 >400 >400 (S)-DHPA >400 350 >400 >400 >400 Effect of selected compounds of formula (I) (100 µg/ml) on multiplication of herpes simplex virus type 1 (HSV-1) (strain KOS) and vaccinia virus (VV) in PRK cell cultures Compound Titer of HSV-1 (log₁₀ PFU/ml) Titer of VV (log₁₀PFU/ml) , 1 h 24 h 48 h 72 h 1 h 24 h 48 h 72 h No. 1 < 1.3 3.3 4.0 3.9 1.7 1.7 1.2 1.5 No. 2 - <1.3 <1.3 (<6.0) - 2.2 2.2 2.0 (<1.3) 1.8 No. 4 - <1.3 <1.3 (4.3) - - - <1.3 (<1.3) - No. 5 - <1.3 2.8 (6.04) - 1.6 1.8 <1.3 (<1.3) 1.5 No. 6 - <1.3 3.34 (6.36) - - - 5.54 - Control 1.3 6.7 6.5 7.0 2.7 6.1 6.5 6.3 (S)-DHPA 1.3 6.5 6.9 6.9 2.0 4.7 5.2 5.5

Claims: Claims for the following Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL, SE A therapeutic composition for use in the treatment of virus diseases, which comprises as an active ingredient a phosphonylmethoxyalkyladenine of the following general formula (I) wherein R¹ is methylene, -CH(OH)-CH₂- or H-CH₂OH and R² is a hydroxyl group, and, wherein, when R¹ is different from methylene, R¹ and R² may be linked with each other to form a cyclic ester group, said adenine derivative being in (RS) or (S) form when R¹ is different from methylene and further being in the form of a free acid or a salt thereof. The composition as claimed in claim 1, comprising (S)-9-hydroxy-2-phosphonylmethoxypropyl)adenine as an active ingredient. Use of compounds of formula (I) for the manufacture of a therapeutic composition against virus diseases. Claims for the following Contracting State : AT A method of manufacturing a therapeutic composition for use in the treatment of virus diseases, which comprises the step of incorporating therein as an active ingredient a phosphonylmethoxyalkyladenine of the following general formula (I) wherein R¹ is methylene, -CH(OH)-CH₂- or H-CH₂OH and R² is a hydroxyl group, and, wherein, when R¹ is different from methylene, R¹ and R² may be linked with each other to form a cyclic ester group, said adenine derivative being in (RS) or (S) form when R¹ is different from methylene and further being in the form of a free acid or a salt thereof. The method as claimed in claim 1, comprising the step of incorporating (S)-9-hydroxy-2-phosphonylmethoxypropyl)adenine therein as an active ingredient. Use of compounds of formula (I) for the manufacture of a therapeutic composition against virus diseases.

Inventors: DE CLERCQ, ERIK, HOLY, ANTONIN INSTITUTE OF ORGANIC CHEM. AND, ROSENBERG, IVAN INSTITUTE OF ORGANIC CHEM. AND
Applicants: CESKOSLOVENSKA AKADEMIE VED, STICHTING REGA V.Z.W.
Application year: 1986
Main classification: A61K31
IPC codes: C07D473, A61K31, A61P31, C07F9