RHIZOXIN DERIVATIVES, THEIR PREPARATION AND PHARMACEUTICAL USE

Abstract: Acylated derivatives of rhizoxin and of rhizoxin-2-ene may be prepared by reacting rhizoxin or rhizoxin-2-ene with an acid or reactive derivative thereof. They have excellent anti-tumour activity.

Description: The present invention relates to a series of novel derivatives of the compound rhizoxin. The invention also provides a process for preparing such compounds and methods and compositions for using them. Rhizoxin itself is a known substance having the following formula: Rhizoxin itself and its acetate were disclosed in J. Antibiotics, 37, 354 (1984) and the anti-tumour activity of rhizoxin was disclosed in the Abstracts of the 43rd General Meeting of the Japanese Cancer Society, page 243, Title No. 1005 (1984). Rhizoxin-2-ene was reported on 18th December 1984 to the 1984 International Chemical Congress of the Pacific Basin Society, Honolulu, Hawaii. We have now discovered a series of rhizoxin derivatives which have far better anti-tumour activity than rhizoxin and its acetate and which have a lower toxicity than rhizoxin itself. The compounds of the present invention are those compounds of formula (I): wherein: R represents a C₃-C₁₇ alkyl group and A represents an extra carbon-carbon bond or an oxygen atom, and pharmaceutically acceptable salts and ring-closed lactones thereof. The ring-closed lactone corresponding to the compound of formula (I) may be represented by the formula (II): wherein R and A are as defined above. Compounds of formula (II) may be prepared by reacting a compound of formula (III): (wherein A is as defined above) with a carboxylic acid of formula (IV): (wherein R is as defined above) or a reactive derivative thereof. Salts of the compounds of formula (I) may be prepared by reacting the compound of formula (II) with an appropriate base. The free acid of formula (I) may be prepared by reacting such a salt with an acid. The invention also provides a pharmaceutical composition comprising an anti-tumour agent in admixture with a pharmaceutically acceptable carrier or diluent, wherein the anti-tumour agent is at least one compound selected from the group consisting of compounds of formula (I), compounds of formula (II) and pharmaceutically acceptable salts of said compounds of formula (I). The compounds of the invention may be used for treating an animal, especially mammal, including human being, suffering from tumours, by administering thereto an effective amount of an anti-tumour agent, wherein said anti-tumour agent is at least one compound of formula (I), compounds of formula (II) or a pharmaceutically acceptable salts of said compounds of formula (I). In the compounds of the invention, R represents a C₃-C₁₇ alkyl group. Such an alkyl group may be a straight or branched chain group. Examples of such groups include the propyl, isopropyl, butyl, sec-butyl, isobutyl, t-butyl, pentyl, isopentyl, neopentyl, t-pentyl, hexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, isohexyl, heptyl, 1-methylhexyl, 2-methylhexyl, 5-methylhexyl, 3-ethylpentyl, octyl, 2-methylheptyl, 6-methylheptyl, 2-ethylhexyl, 2-ethyl-3-methylpentyl, 3-ethyl-2-methylpentyl, nonyl, 2-methyloctyl, 7-methyloctyl, 4-ethylheptyl, 3-ethyl-2-methylhexyl, 2-ethyl-1-methylhexyl, decyl, 2-methylnonyl, 8-methylnonyl, 5-ethyloctyl, 3-ethyl-2-methylheptyl, 3,3-diethylhexyl, undecyl, 2-methyldecyl, 9-methyldecyl, 4-ethylnonyl, 3,5-dimethylnonyl, 3-propyloctyl, 5-ethyl-4-methyloctyl, dodecyl, 1-methylundecyl, 10-methylundecyl, 3-ethyldecyl, 5-propylnonyl, 3,5-diethyloctyl, tridecyl, 11-methyldodecyl, 7-ethylundecyl, 4-propyldecyl, 5-ethyl-3-methyldecyl, 3-pentyloctyl, tetradecyl, 12-methyltridecyl, 8-ethyldodecyl, 6-propylundecyl, 4-butyldecyl, 2-pentylnonyl, pentadecyl, 13-methyltetradecyl, 10-ethyltridecyl, 7-propyldodecyl, 5-ethyl-3-methyldodecyl, 4-pentyldecyl, hexadecyl, 14-methylpentadecyl, 6-ethyltetradecyl, 4-propyltridecyl, 2-butyldodecyl, heptadecyl, 15-methylhexadecyl, 7-ethylpentadecyl, 3-propyltetradecyl and 5-pentyldodecyl groups. Of these groups, we prefer the C₇-C₁₃ alkyl groups, whether straight or branched chain groups. Compounds of formula (I) are free acids and hence can form salts with bases. Provided that the resulting salt is pharmaceutically acceptable, which, as is well-known in the art, means that the salt does not have reduced (or unacceptably reduced) activity or increased (or unacceptably increased) toxicity as compared with the parent acid, there is no restriction on the nature of the cation forming the salt. Examples of suitable salts include metal salts, salts with amino acids and salts with ammonia and organic amines. Examples of suitable metal salts include salts with: alkali metals, such as sodium or potassium; alkaline earth metals, such as calcium or magnesium; and salts with other pharmaceutically acceptable metals, such as aluminium, iron, zinc, copper, nickel and cobalt. However, the preferred salts are those with alkali metals, alkaline earth metals and aluminium, and the most preferred salts are the sodium, potassium, calcium and aluminium salts. Examples of amino acids with which the compounds of formula (I) may form salts include such basic amino acids as arginine, lysine, histadine, α,ϒ-diaminobutyric acid and ornithine. Examples of amines with which the compounds of formula (I) may form salts include t-octylamine, dibenzylamine, dicyclohexylamine, morpholine, D -phenylglycine alkyl esters and D -glucosamine. In naming the compounds of the invention, they are named semi-systematically in accordance with the recommendations of the International Union of Pure and Applied Chemistry, "Nomenclature of Organic Chemistry" Section F, taking rhizoxin as the base name. Thus, compounds of formula (II) where A represents an oxygen atom are simply esters of rhizoxin with an acid of formula RCOOH and these are thus named as rhizoxin-13-yl acylates. The ring-opened analogue of rhizoxin is called rhizoxin-5b-oic acid and thus compounds of formula (I) where A represents an oxygen atom are named as the 13-acyloxy derivatives of this, i.e. 13-acyloxy-13-dehydroxyrhizoxin-5b-oic acids. Compounds of formula (II) where A represents an extra carbon-carbon bond are regarded as derivatives of rhizoxin-2-ene, more formally 2,3-deoxyrhizoxin-2-ene. Hence, compounds of formulae (I) and (II) where A represents such a bond are named as 13-acyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acids and 2,3-deoxyrhizoxin-2-en-13-yl acylates, respectively. Examples of compounds of the present invention are given in the following list and the compounds of the invention are hereinafter, where appropriate, identified by the numbers assigned to them in this list. 1. 13-butyryloxy-13-dehydroxyrhizoxin-5b-oic acid 2. rhizoxin-13-yl butyrate 3. 13-valeryloxy-13-dehydroxyrhizoxin-5b-oic acid 4. rhizoxin-13-yl valerate 5. 13-isovaleryloxy-13-dehydroxyrhizoxin-5b-oic acid 6. rhizoxin-l3-yl isovalerate 7. 13-hexanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 8. rhizoxin-13-yl hexanoate 9. 13-(3,3-dimethylbutyryloxy)-13-dehydroxyrhizoxin-5b-oic acid 10. rhizoxin-13-yl 3,3-dimethylbutyrate 11. 13-heptanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 12. rhizoxin-13-yl heptanoate 13. 13-(5-methylhexanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 14. rhizoxin-13-yl 5-methylhexanoate 15. 13-(4-methylhexanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 16. rhizoxin-13-yl 4-methylhexanoate 17. 13-octanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 18. rhizoxin-13-yl octanoate 19. 13-(6-methylheptanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 20. rhizoxin-13-yl 6-methylheptanoate 21. 13-(4-ethylhexanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 22. rhizoxin-13-yl 4-ethylhexanoate 23. 13-nonanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 24. rhizoxin-13-yl nonanoate 25. 13-(4-ethyl-3-methylhexanoyloxy)-13-dehydroxy-rhizoxin-5b-oic acid 26. rhizoxin-13-yl 4-ethyl-3-methylhexanoate 27. 13-(7-methyloctanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 28. rhizoxin-13-yl 7-methyloctanoate 29. 13-decanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 30. rhizoxin-13-yl decanoate 31. 13-(8-methylnonanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 32. rhizoxin-13-yl 8-methylnonanoate 33. 13-(5-ethyloctanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 34. rhizoxin-13-yl 5-ethyloctanoate 35. 13-undecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 36. rhizoxin-13-yl undecanoate 37. 13-(9-methyldecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 38. rhizoxin-13-yl 9-methyldecanoate 39. 13-(6-ethylnonanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 40. rhizoxin-13-yl 6-ethylnonanoate 41. 13-dodecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 42. rhizoxin-13-yl dodecanoate 43. 13-(10-methylundecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 44. rhizoxin-13-yl 10-methylundecanoate 45. 13-(6-ethyl-5-methylnonanoyloxy)-13-dehydroxy-rhizoxin-5b-oic acid 46. rhizoxin-13-yl 6-ethyl-5-methylnonanoate 47. 13-tridecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 48. rhizoxin-13-yl tridecanoate 49. 13-(11-methyldodecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 50. rhizoxin-13-yl 11-methyldodecanoate 51. 13-(4,6-diethylnonanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 52. rhizoxin-13-yl 4,6-diethylnonanoate 53. 13-tetradecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 54. rhizoxin-13-yl tetradecanoate 55. 13-(8-ethyldodecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 56. rhizoxin-13-yl 8-ethyldodecanoate 57. 13-pentadecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 58. rhizoxin-13-yl pentadecanoate 59. 13-(9-ethyltridecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 60. rhizoxin-13-yl 9-ethyltridecanoate 61. 13-hexadecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 62. rhizoxin-13-yl hexadecanoate 63. 13-(8-propyltridecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 64. rhizoxin-13-yl 8-propyltridecanoate 65. 13-heptadecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 66. rhizoxin-13-yl heptadecanoate 67. 13-(7-ethylpentadecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 68. rhizoxin-13-yl 7-ethylpentadecanoate 69. 13-octadecanoyloxy-13-dehydroxyrhizoxin-5b-oic acid 70. rhizoxin-13-yl octadecanoate 71. 13-(8-ethylhexadecanoyloxy)-13-dehydroxyrhizoxin-5b-oic acid 72. rhizoxin-13-yl 8-ethylhexadecanoate 73. 13-heptanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 74. 2,3-deoxyrhizoxin-2-en-13-yl heptanoate 75. 13-octanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 76. 2,3-deoxyrhizoxin-2-en-13-yl octanoate 77. 13-nonanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 78. 2,3-deoxyrhizoxin-2-en-13-yl nonanoate 79. 13-(7-methyloctanoyloxy)-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 80. 2,3-deoxyrhizoxin-2-en-13-yl 7-methyloctanoate 81. 13-decanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 82. 2,3-deoxyrhizoxin-2-en-13-yl decanoate 83. 13-(8-methylnonanoyloxy)-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 84. 2,3-deoxyrhizoxin-2-en-13-yl 8-methylnonanoate 85. 13-undecanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 86. 2,3-deoxyrhizoxin-2-en-13-yl undecanoate 87. 13-(9-methyldecanoyloxy)-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 88. 2,3-deoxyrhizoxin-2-en-13-yl 9-methyldecanoate 89. 13-dodecanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 90. 2,3-deoxyrhizoxin-2-en-13-yl dodecanoate 91. 13-tridecanoyloxy-13-dehydroxy-2,3-deoxyrhizoxin-2-en-5b-oic acid 92. 2,3-deoxyrhizoxin-2-en-13-yl tridecanoate Of the compounds listed above, Compounds Nos. 18. 29, 30, 42 and 54 are particularly preferred. The compounds of the present invention can exist in the form of various geometrical isomers, depending upon the configuration of the various substituent groups, and also, because of the presence of a number of asymmetric carbon atoms, can exist in the form of various optical isomers. These isomers, and mixtures of these isomers, are all represented herein by a single general formula. However, the present invention embraces both the individual isolated isomers, as well as mixtures thereof. In general, we prefer that the compounds of the invention should have the same configuration as rhizoxin. The ring-closed lactone derivatives of formula (II) can be produced by reacting the compound of formula (III) defined above, which is either rhizoxin (A represents an oxygen atom) or rhizoxin-2-ene (A represents an extra carbon-carbon bond), with a carboxylic acid of formula (IV) or a reactive derivative thereof. This reaction is a simple and conventional acylation reaction and may be carried out by methods well-known in the art for such acylation reactions. Where the carboxylic acid of formula (IV) as such is employed, the reaction is preferably effected in the presence of a condensing agent which has a dehydrating activity. Suitable such condensing agents include dicyclohexylcarbodiimide and carbonyldiimidazole. The reaction is preferably effected in the presence of a solvent, the nature of which is not critical, provided that it has no adverse effect on the reaction. Examples of suitable solvents include: aromatic hydrocarbons, such as benzene, toluene or xylene; aliphatic hydrocarbons, such as hexane, heptane, cyclohexane or petroleum ether: halogenated hydrocarbons, especially halogenated aliphatic hydrocarbons, such as chloroform, carbon tetrachloride or methylene chloride; and ethers such as tetrahydrofuran or dioxane. The reaction will take place over a wide range of temperatures and the exact temperature chosen is not critical. We normally find it convenient to carry out the reaction at a temperature in the range from 0 to 40°C, preferably at about room temperature. Examples of suitable reactive derivatives of the carboxylic acid of formula (IV) include the acid halides, acid anhydrides, mixed acid anhydrides, active esters and active amides, of which the acid halides (such as the acid chloride or acid bromide) and the acid anhydrides (including mixed acid anhydrides) are preferred. Where an acid halide is employed, the reaction is preferably effected in the presence of an inert solvent and in the presence of an acid-binding agent. The nature of the solvent employed is not critical to the present invention, provided that it has no adverse effect upon the reaction. Preferred solvents are organic solvents, for example: aromatic hydrocarbons, such as benzene, toluene or xylene; halogenated aliphatic hydrocarbons, such as chloroform, methylene chloride or 1,1,2-trichloroethane; ethers, such as diethyl ether, tetrahydrofuran or dioxane; dialkylamides of aliphatic acids, such as dimethylformamide or dimethylacetamide; nitriles, such as acetonitrile; ketones, such as acetone; dimethyl sulphoxide; and pyridine. The function of the acid-binding agent is to remove the hydrogen halide produced in the reaction and any compound capable of reacting with the hydrogen halide and removing it from the reaction system may be employed. Examples of suitable acid-binding agents include: alkali metal hydroxides, such as sodium hydroxide or potassium hydroxide; alkali metal carbonates, such as sodium carbonate or potassium carbonate; and organic bases, such as triethylamine, pyridine, 4-dimethylaminopyridine or 1-methylimidazole. The reaction may be carried out over a wide range of temperatures, but preferably at a temperature from -10°C to +130°C. Where an acid anhydride of the compound of formula (IV) is employed, the reaction is preferably effected in the presence of an additional solvent. However, if a sufficient excess of the acid anhydride is used, no additional solvent is necessary. Where a solvent is employed, its nature is not critical, provided that it has no adverse effect upon the reaction. Examples of suitable solvents include: aromatic hydrocarbons, such as benzene, toluene or xylene; and ethers, such as dioxane, tetrahydrofuran and diethylene glycol dimethyl ether. The reaction will take place over a wide range of temperatures, but a temperature within the range from room temperature to 160°C is preferred. The product of this reaction is the lactone of formula (II). Pharmaceutically acceptable salts of the carboxylic acid of formula (I) can be prepared by reacting this lactone of formula (II) with a base. This is a conventional reaction for forming a salt from a lactone and may be carried out using techniques well-known in the art. For example, metal salts of the carboxylic acid of formula (I) can be prepared by reacting the lactone of formula (II) with a hydroxide or carbonate of the appropriate metal, preferably in an aqueous solvent. The nature of this solvent is not critical, provided that it has no adverse effect upon the reaction. Suitable solvents include water itself and mixtures of water with one or more organic solvents, for example: an alcohol, such as methanol or ethanol; an ether, such as ethylene glycol dimethyl ether or dioxane; a ketone, such as acetone; or another solvent such as hexane, ethyl acetate, dimethylformamide, dimethyl sulphoxide or pyridine. A mixture of a hydrophilic organic solvent with water is particularly preferred. The reaction temperature is not critical and we therefore normally prefer to carry out the reaction at about room temperature. However, if desired, it may be conducted whilst gently heating. In order to avoid opening the lactone formed between the carbon atoms at positions 15 and 1, it is preferred that the ring-opening reaction should take place under relatively mild conditions, e.g. using a relatively dilute solution of the base and/or at relatively low temperatures, e.g around room temperature. An amine salt of the carboxylic acid of formula (I) may be prepared by reacting the lactone of formula (II) with an amine, preferably in an aqueous solvent. The solvent employed is not critical, provided that it has no adverse effect upon the reaction. Suitable solvents include water itself and mixtures of water with one or more organic solvents, for example: an alcohol, such as methanol or ethanol; an ether, such as tetrahydrofuran; a nitrile, such as acetonitrile; or a ketone, such as acetone. The preferred solvent is aqueous acetone. The reaction is preferably effected at a pH value of from 7 to 8.5 and, although the reaction temperature is not particularly critical, we prefer a relatively low temperature in order to avoid side reactions. Accordingly, the temperature is preferably below room temperature, more preferably from 5 to 10°C. The reaction goes immediately to completion. The amine salt may also be produced by a salt-exchange reaction, that is to say by adding a mineral acid salt (e.g. the hydrochloride) of the desired amine to an aqueous solution of an metal salt of the compound of formula (I). An amino acid salt of the carboxylic acid of formula (I) can be prepared by contacting the lactone of formula (II) with an appropriate amino acid, preferably in an aqueous solvent. The solvent employed is not critical, provided that it has no adverse effect upon the reaction. Suitable solvents are aqueous solvents, such as water itself and mixtures of water with one or more organic solvents, for example: an alcohol, such as methanol or ethanol; or an ether, such as tetrahydrofuran. The reaction temperature is not critical, but best results are obtained by heating the reagents, preferably at a temperature of from 50 to 60°C. The free acids of formula (I) can be prepared by contacting a salt thereof with an acid. The reaction may be carried out by conventional means, as are well-known in this art. For example, the reaction is preferably effected in the presence of a solvent, the nature of which is not critical, provided that it has no adverse effect upon the reaction. Suitable solvents include, for example, methanol, acetone, dimethylformamide and dimethylacetamide. The salt of the carboxylic acid (I) is dissolved in such a solvent, and then a stoichiometric equivalent or a slight excess of an acid is added. There is no particular limitation on the nature of the acid to be used and any organic or inorganic acid may be employed, provided that it does not have any adverse effect upon the desired compound. Suitable acids include trifluoroacetic acid, hydrochloric acid and sulphuric acid. Alternatively, the compounds of formula (I) may be prepared by reacting the ring-opened lactone corresponding to the compound of formula (III) with a compound of formula (IV) or reactive derivative thereof. However, this route is not presently preferred. The resulting compounds of the invention, prepared by any of the methods described above, can be recovered from the reaction mixtures and, if desired, further purified by any conventional technique or by a combination of such techniques. For example, one suitable recovery procedure comprises: pouring the reaction mixture into water; extracting the product with a water-immiscible solvent, such as benzene, diethyl ether or ethyl acetate; and then evaporating off the solvent, if necessary after drying the extract, to afford the desired compound. This may, if desired, be purified by an adsorption chromatography technique, using an adsorbent such as activated carbon or silica gel, by ion-exchange chromatography, by gel filtration with a suitable adsorbent, such as Sephadex (trade mark) or by recrystallization from an organic solvent, such as diethyl ether, ethyl acetate or chloroform. Of course, a combination of these techniques may be employed, if appropriate. The starting material used in the processes of the invention is rhizoxin, which may be prepared by cultivating a rhizoxin-producing fungus, e.g. of the genus Rhizopus, in a culture medium therefor and separating rhizoxin from the cultured broth. The fungus employed is preferably of the species Rhizopus chinensis and is most preferably Rhizopus chinensis SANK 21584. Rhizopus chinensis SANK 21584 grows at temperatures ranging from 20°C to 47°C. It grows very rapidly on the potato-dextrose agar medium at a temperature of 26°C. The floccose mycelia develop well. With the formation of sporangiospores, the colour of the hyphae turns from off-white to dark brown. Most of the sporangiospores are unbranched and from one to several of them are formed vertically at the sites where the rhizoids develop with a simple shape. The size of the sporangiophores is 100-600 x 7-12 µm. The sporangia are globose to subglobose, and their size is 50-120 µm; they become brown as they mature. The columellae are subglobose to ellipsoid and the size is 15-50 µm. The sporangiospores are brown and subglobose to ellipsoid and the size is 6-10 x 3-8 µm. The clamidospores which are formed take variable shapes. No zygospore is formed. Identification of strain SANK 21584 was carried out with reference to the following literature, and it was identified to be a strain of Rhizopus chinensis Saito: T. Inui et al, "Taxonomical Studies on Genus Rhizopus", J. Gen. Appl. Microbiol., 11 , 1-108 (1965); D. H. Ellis, "Sporangiospore Ornamentation of Thermophilic Rhizopus Species and Some Allied Genera", Mycologia, 73 , 511-523 (1981). Rhizopus chinensis SANK 21584 was deposited with the Fermentation Research Institute, Tokyo, Japan on 14th February 1985 under the accession No. FERM P-8093 and was redeposited under the conditions stipulated by the Budapest Treaty on 15th February 1986 under the accession no. BP-989. The compounds of the present invention have shown excellent anti-tumour activity against implanted P 388 luekemia cells in mice and can thus be employed as anti-tumour agents against such tumours in animals, especially warm-blooded animals such as humans. As will be demonstrated hereafter in the biological activity data forming Example 6, the compounds of the invention show an impressive ability to increase the lifespan of test animals experimentally implanted with such tumours, as measured by the Index of Increase in Life Span (ILS). The ILS represents the effect in the animal or patient of a balance between the curative effect of the drug and its toxicity and there is, therefore, not a simple relationship between dosage and ILS. Since a smaller ILS represents a shorter lifespan for the animal or patient (regardless of whether the death is caused by the tumour or the toxicity of the drug), whilst a higher ILS represents a longer lifespan, the critical feature of a potential anti-tumour drug is to maximize its ILS value, regardless of the dosage at which this maximum ILS is reached; in this respect, the criteria for assessing the worth of anti-tumour drugs differ from those used for most other drugs, including antibiotics, hypotensive agents etc. The compounds of the invention show ILS values which indicate a potentially valuable anti-tumour activity coupled with relatively low toxicity and which are significantly better than those of rhizoxin and its known derivatives. The compounds may be administered by any suitable route, for example the parenteral route (e.g. by intravenous, subcutaneous or intramuscular injection) or by suppository, or by the oral route (for example in the form of a tablet, capsule, powder or granule). If desired, the compound of the invention may be administered as such, but it is preferably employed in association with a conventional pharmaceutically acceptable carrier or diluent, appropriate to the particular route of administration. For example, the composition may contain suspending agents, stablizing agents or dispersing agents and it may be provided as a powder which, prior to administration, is dissolved in a suitable solvent, for example a pyrogen-free sterilized aqueous solvent. Such a powdered preparation may, for example, be produced by pipetting an acetone solution of the compound into a vial, adding water thereto and then lyopholizing the mixture. Compositions for oral use may be provided as tablets, capsules, powders, granules or syrups containing an appropriate amount of the compound of the invention. Compositions for injection are preferably provided as an ampoule containing a unit dose or as a vial containing multiple doses. If desired, the compounds of the invention may be used together with other anti-cancer agents, for example drugs of the nitrosourea series, such as ACNU or BCNU, cisplastin, 5-FU, daunomycin, adriamycin, mitomycin C or etoposide. The dosage of the compounds of the invention will vary, depending upon the severity and nature of the disease, as well as the route, frequency and period of administration. However, a suitable dose for an adult human would be in the range of from 1 to 100 mg per day, which may be administered in a single dose or in divided doses. The preparation and biological activity of the compounds of the present invention are further illustrated by the following Examples. EXAMPLE 1 Rhizoxin-13-vl Decanoate (Compound No. 30) 250 mg (3.0 mmole) of pyridine and a catalytic amount of 4-dimethylaminopyridine were added, whilst ice-cooling, to a solution of 625 mg (1 mmole) of rhizoxin and 285 mg (1.5 mmole) of decanoyl chloride in 10 ml of benzene, and the mixture was agitated at room temperature for about 30 minutes. At the end of this time, the reaction mixture was washed, in turn, with dilute hydrochloric acid and then water. It was then dried over anhydrous sodium sulphate, and the solvent was removed by evaporation under reduced pressure. The residue was then purified by silica gel column chromatography, eluted with a 95:5 by volume mixture of benzene and acetone, to afford a crystalline compound, which was recrystallized from methanol to give about 500 mg of the title compound as white crystals melting at 168-169°C. Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (43,700), 309 (55,800), 323 (41,100). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹: 2940, 2855, 1740, 1580, 1450, 1225, 1190, 1110, 1090. Electron impact mass spectrum: 779 (M+), 232. EXAMPLE 2 Following the same procedure as described in Example 1, but employing different acid chlorides, the following compounds were also prepared: (1) Rhizoxin-13-yl butyrate (Compound No.2) R = -CH₂CH₂CH₃: White powder: Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (44,400), 309 (56,600), 323 (41,700). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 2970, 2940, 2870, 1740, 1580, 1450, 1380, 1225, 1190, 1175, 1110, 1090. Electron impact mass spectrum: 625 (M+), 232 (C-17 to C-26a segment). (2) Rhizoxin-13-yl isovalerate (Compound No.6) R = -CH₂CH(CH₃)₂: White powder: Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (42,800), 309 (55,200), 323 (40,300). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 2970, 2940, 2870, 1740, 1580, 1460, 1225, 1190, 1110, 1090. Electron impact mass spectrum: 751 (M + ), 232. (3) Rhizoxin-13-yl octanoate (Compound No.18) R = -(CH₂)₆CH₃: White powder: Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (43,400), 309 (55,200), 323 (40,700). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 2970, 2940, 2855, 1740, 1580, 1450, 1225, 1190, 1110, 1090. Electron impact mass spectrum: 751 (M + ), 232. (4) Rhizoxin-13-yl dodecanoate (Compound No.42) R = -(CH₂)₁₀CH₃: white crystals, melting at 141-142°C. Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 296 (42,800), 309 (54,600), 323 (40,300). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 2940, 2855, 1740, 1580, 1455, 1225, 1190, 1110, 1090. Electron impact mass spectrum: 807 (M + ), 232. (5) Rhizoxin-13-yl octadecanoate (Compound No.70) R = -(CH₂)₁₆CH₃: White crystals, melting at 62-64°C. Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (40,500), 309 (51,500), 323 (38,100). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 2940, 2855, 1740, 1580, 1455, 1225, 1190, 1110, 1090. Electron impact mass spectrum: 891 (M + ), 232. (6) Rhizoxin-13-yl tetradecanoate (Compound No.54) R = -(CH₂)₁₂CH₃: White crystals, melting at 116.5-117.5°C. Ultraviolet Absorption Spectrum (ethanol) λ max nm (ε): 298 (41800), 310 (53400), 324 (39800). Infrared Absorption Spectrum (KBr) ν max cm - ¹ : 2925, 2853, 1736, 1231, 1187, 1109, 1084, 983. Electron impact mass spectrum : 835 (M + ), 232. EXAMPLE 3 Sodium 13-decanoyloxy-13-dehydroxyrhizoxin-5b-oate (Sodium salt of Compound No. 29) 2 ml (0.2 mmole) of a 0.1N aqueous solution of sodium hydroxide were added dropwise, whilst stirring, to a solution of 156 mg (0.2 mmole) of rhizoxin-13-yl decanoate (prepared as described in Example 1) in 8 ml of ethylene glycol dimethyl ether, and the mixture was agitated at room temperature for 10 minutes. The organic solvent was then evaporated off under reduced pressure at room temperature, and the residual aqueous solution was lyophilized, to give 164 mg of the title compound as a white powder melting at 115-120°C. Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (40,200), 309 (51,500), 323 (37,900). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 3300 (broad), 2940, 2855, 1740, 1575, 1450, 1410, 1190, 1110, 1090. Electron impact mass spectrum: 780 (M + - ONa), 779 (M + -HONa), 232 (base peak). EXAMPLE 4 13-Decanoyloxy-13-dehydroxyrhizoxin-5b-oic acid (Compound No.29) A sample of the sodium 13-decanoyloxy-13-dehydroxyrhizoxin-5b-oate (prepared as described in Example 3) was dissolved in methanol, and 1.1 equivalents of 0.1N aqueous hydrochloric acid were added thereto. The mixture was then subjected to thin layer chromatography (Kiesel gel 60 F-254, Merck) and showed a spot corresponding to the free carboxylic acid, the title compound. Using as the developing solvent a 4:1 by volume mixture of benzene and isopropanol, the Rf value was 0.40; using as developing solvent a 1:1 by volume mixture of benzene and acetone, the Rf value was 0.23. For comparison, the Rf values of rhizoxin in these developing solvents are 0.82 and 0.89, respectively. EXAMPLE 5 2,3-Deoxyrhizoxin-2-en-13-yl decanoate (Compound No. 82) 183 mg (0.3 mmole) of rhizoxin-2-ene (prepared as described hereafter in the Preparation), 100 mg (0.99 mmole) of triethylamine and a catalytic amount of 4-dimethylaminopyridine were dissolved in 10 ml of dry benzene, and then 86 mg (0.45 mmole) of decanoyl chloride were added dropwise, whilst stirring and ice-cooling, to the resulting solution. Stirring was continued for a further 1 hour at room temperature, and then the reaction mixture was washed with water and dried over anhydrous magnesium sulphate. The solvent was then removed by evaporation under reduced pressure. The residue was subjected to silica gel column chromatography, eluted with a 95:5 by volume mixture of benzene and acetone, to give crude crystals, which were recrystallized from methanol, giving 165 mg of the title compound as white crystals, melting at 145-146°C. Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (44,000), 309 (56,900), 323 (41,500). Infrared Absorption Spectrum (CCl₄) ν max cm - ¹ : 2940, 2855, 1735, 1650, 1580, 1450, 1380, 1320, 1210, 1195, 1170, 1110, 1080, 1040, 980, 965. Electron impact mass spectrum: 763 (M + ), 232 (base peak). PREPARATION Rhizoxin-2-ene A slant-full of hyphae from a culture of Rhizopus chinensis (FERM-8093) was inoculated into a 500 ml Erlenmeyer flask containing 100 ml of a culture medium having the following composition (percentages are by weight): pH (before sterilization) 6.8 (which had previously been sterilized at 120°C for 45 minutes). Cultivation was carried out at 28°C for 24 hours, with shaking. 200 ml of the resulting pre-incubated fluid were then transferred to a 500 litre culture jar containing 30 litres of a medium having the composition described above, and the mixture was cultured for 95 hours at 28°C under aeration at the rate of 15 litres per minute, with stirring at 200 rpm and under an internal pressure of 1.0 kg/cm². At the end of this time, the cultured broth was filtered, with the aid of a Celite (trade mark) filter aid, and the filtrate was extracted with an equal volume of ethyl acetate. The solvent was evaporated from the extract, and the residue was subjected to LH20 column chromatography, using acetone as the eluent, to give a fraction containing the title compound. This fraction was then subjected to silica gel column chromatography, using a 85:15 by volume mixture of benzene and acetone as eluent, to give a mixture of the title compound and rhizoxin. Further purification of the mixture using LH20 column chromatography and silica gel column chromatography gave 15 mg of the title compound of about 90% purity and 670 mg of rhizoxin of the same approximate purity from 300 litres of cultured broth. The title compound was finally purified with high pressure liquid chromatography, using ODS, to give the title compound as a white powder, melting at 125-128°C. Ultraviolet Absorption Spectrum (CH₃OH) λ max nm (ε): 297 (48,600), 309 (64,400), 323 (46,300). Infrared Absorption Spectrum (CCl₄) ν max cm-¹ : 3580, 2970, 2940, 2880, 1740, 1720, 1650, 1580, 1450, 1385, 1320, 1310, 1220, 1195, 1170, 1110, 1080, 1050, 980, 965. Electon impact mass spectrum: 609 (M + ), 232 (base peak). EXAMPLE 6 Biological Activity The test animals employed were female mice, 8 weeks of age, of the CDF₁ strain. The mice were divided into groups, each of 5 mice, and all mice within the group were treated identically. Into each mouse was implanted intraperitoneally 1 x 10⁶ cells of the mouse leukemia p-388. The test compounds shown in the following Table were suspended in sterilized physiological saline and the suspension was administered intraperitoneally on the first, fifth and ninth days following implantation of the leukemia cells. The period for which the mice survived was determined. A control group was treated identically, except that no active compound was administered. The anti-tumour effect is shown in the following Table as the increase in life span [ILS (%)], calculated from the following equation: ILS (%) = (Dt/Dc - 1) x 100 where Dt = average number of days survival by the test group; and Dc = average number of days survival by the control group. In this test, Dc was 10.6 days. The compounds of the invention are identified in the following Table by the numbers assigned to them in the foregoing list. Compounds A, B and C are rhizoxin, rhizoxin-2-ene and rhizoxin-13-yl acetate. The results shown above indicate a significant anti-tumour activity in the compounds of the present invention. All of the compounds of the invention have an ILS value within the range from 98 to 241 at least at one dosage level, indicating a significantly better degree of anti-tumour activity than rhizoxin, rhizoxin-2-ene and rhizoxin-13-yl acetate, where the highest values were 73 (at 2 mg/kg/day), 65 (at 8 mg/kg/day) and 38 (at 4 mg/kg/day), respectively. A negative ILS indicates that the average lifespan of the test group was less than that of the control group. Thus, rhizoxin showed its highest ILS at 2 mg/kg/day but, by a dose of 6 mg/kg/day, the toxicity of rhizoxin predominated over its anti-tumour activity. On the other hand, Compound No. 29 did not reach its maximum ILS value until a dose (of those tried) of 64 mg/kg/day and the majority of the other compounds of the invention showed their maximum ILS values at doses of 8 mg/kg/day.

Claims: Compounds of formula (I): wherein: R represents a C₃-C₁₇ alkyl group; and A represents an extra carbon-carbon bond or an oxygen atom, and pharmaceutically acceptable salts and ring-closed lactones thereof. Compounds as claimed in Claim 1, wherein R represents a C₇-C₁₃ alkyl group. Compounds as claimed in Claim 1, wherein R represents a heptyl, nonyl, undecyl or tridecyl group. Compounds as claimed in any one of Claims 1 to 3, wherein A represents an oxygen atom. Compounds of formula (II): wherein: R represents a C₃-C₁₇ alkyl group; and A represents an extra carbon-carbon bond or an oxygen atom. Compounds as claimed in Claims 5, wherein R represents a C₇-C₁₃ alkyl group. Compounds as claimed in Claims 5, wherein R represents a heptyl, nonyl, undecyl or tridecyl group. Compounds as claimed in any one of Claims 5, to 7, wherein A represents an oxygen atom. Rhizoxin-13-yl octanoate. 13-Decanoyloxy-13-dehydroxyrhizoxin-5b-oic acid and pharmaceutically acceptable salts thereof. Rhizoxin-13-yl decanoate. Rhizoxin-13-yl dodecanoate. Rhizoxin-13-yl tetradecanoate. Rhizoxin-13-yl hexadecanoate. A pharmaceutical composition comprising an anti-tumour agent in admixture with a pharmaceutically acceptable carrier or diluent, wherein the anti-tumour agent is at least one compound as claimed in any one of the preceding Claims. The use for the manufacture of a medicament for the treatment of tumours of a compound as claimed in any one of Claims 1 to 14. A process for preparing a compound of formula (II), as defined in any one of Claims 5 to 10, which comprises reacting a compound of formula (III): (wherein A is as defined in Claims 5) with a carboxylic acid of formula (IV): (wherein R is as defined in Claims 5) or a reactive derivative thereof. A process for preparing a salt of a compound of formula (I), as defined in any one of Claims 1 to 4, which comprises reacting a compound of formula (II): (where R and A are as defined in Claim 1) with a base. A process for preparing a compound of formula (I), as defined in any one of Claims 1 to 4, which comprises reacting a salt thereof with an acid. CLAIMS FOR THE CONTRACTING STATE: AT A process for preparing compounds of formula [wherein: R represents a C₃-C₁₇ alkyl group; and A represents an extra carbon-carbon bond or an oxygen atom], and pharmaceutically acceptable salts and ring-closed lactones thereof, which process comprises the steps: (a) reacting a compound of formula (III): (wherein A is as defined above) with a carboxylic acid of formula (IV): (wherein R is as defined above) or a reactive derivative thereof, to give a compound of formula (II): (where R and A are as defined above); (b) optionally reacting said compound of formula (II) with a base, to give a salt of said compound of formula (I); and (c) optionally reacting said salt with an acid, to give said compound of formula (I). A process as claimed in Claim 1, wherein R in said carboxylic acid of formula (IV) represents a C₇-C₁₃ alkyl group. A process as claimed in Claim 1, wherein R in said carboxylic acid of formula (IV) represents a heptyl, nonyl, undecyl or tridecyl group. A process as claimed in any one of the preceding Claims, wherein said compound of formula (III) is rhizoxin itself. A process as claimed in any one of Claims 1 to 4, in which only step (a) is effected, to prepare said compound of formula (II). A process as claimed in Claim 1, in which said compound of formula (III) is rhizoxin itself and said carboxylic acid of formula (IV) is octanoic acid, and only step (a) is effected, to give rhizoxin-13-yl octanoate. A process as claimed in Claim 1, in which said compound of formula (III) is rhizoxin itself and said carboxylic acid of formula (IV) is decanoic acid, and steps (a) and (b) and optionally (c) are effected, to give 13-decanoyloxy-13-dehydroxyrhizoxin-5b-oic acid or a pharmaceutically acceptable salt thereof. A process as claimed in Claim 1, in which said compound of formula (III) is rhizoxin itself and said carboxylic acid of formula (IV) is decanoic acid, and only step (a) is effected, to give rhizoxin-13-yl decanoate. A process as claimed in Claim 1, in which said compound of formula (III) is rhizoxin itself and said carboxylic acid of formula (IV) is dodecanoic acid, and only step (a) is effected, to give rhizoxin-13-yl dodecanoate. A process as claimed in Claim 1, in which said compound of formula (III) is rhizoxin itself and said carboxylic acid of formula (IV) is tetradecanoic acid, and only step (a) is effected, to give rhizoxin-13-yl tetradecanoate. The use for the manufacture of a medicament for the treatment of tumours of a compound of formula (I), a compound of formula (II) or a salt of a compound of formula (I), as defined in any one of the preceding Claims.

Inventors: ARAKAWA, MASAO, IWASAKI, SHIGEO, NAMIKOSHI, MICHIO, OKUDA, SHIGENOBU, SHIMIZU, FUSAAKI
Applicants: SANKYO COMPANY LIMITED
Application year: 1986
Main classification: C07D493
IPC codes: A61K31, C07D493, A61P35