NOVEL LL-METHYLENE-OESTR-15-ENES, PROCESSES FOR THEIR PREPARATION, AND PHARMACEUTICAL COMPOSITIONS

Abstract: The present invention is concerned with novel 11-­ methylene-oestr-15-enes of the formula: wherein R₁ = H or acyl with 1-18 C-atoms, preferably H, R₂ = ethinyl, vinyl, chloroethinyl, allyl or propinyl; ethinyl being preferred, and R₃ = O or H₂, preferably O. Further the present invention is concerned with pro­ cesses for the preparation of the above compounds and with pharmaceutical compositions comprising these compounds. The compounds possess powerful gestagenic properties and only slight androgenic side-effects.

Description: NOVEL 11-METHYLENE-OESTR-15-ENES, PROCESSES FOR THEIR PREPARATION, AND PHARMACEUTICAL COMPOSITIONS The invention relates to 11-methylene-oestr-15-enes, to processes for their preparation and to pharmaceutical compositions which comprise these oestrenes. The invention in particular relates to ll-methylene-oestra-4,15dienes which possess an unsaturated hydrocarbon group in the 1 7tt-position. Such steroids are known. Thus, EP-A-0,051,762 describes 11-methylene-18-methyl-oestra-4,15-dien-3-ones which in the ila-position are substituted by an ethinyl, chloroethinyl or propinyl group and additionally possess a 17ss-ORs group, wherein R1 represents H or an acyl group. These steroids possess powerful gestagenic properties and only slight androgenic side-effects. A new group of ll-methylene-oestr-l5-enes having valuable biological properties has now been found. These new 11-methylenesteroids have the general formula-I: EMI2.1 (formula I) wherein R1 H or acyl with 1-18 C-atoms, preferably H, R2 - ethinyl, vinyl, chloroethinyl, allyl or propinyl; ethinyl being preferred, and Ra - O or H2, preferably 0. The acyl group with 1-18 C-atoms can be derived from a saturated or unsaturated organic carboxylic acid. As examples of such carboxylic acids there may be mentioned formic acid, acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, caproic acid, oenanthic acid, caprylic acid, pelargonic acid, capric acid, undecylic acid, lauric acid, tridecylic acid, nyristic acid, pentadecylic acid, oleic acid, palmitic acid, stearic acid, adamantanecarboxylic acid, trimethylacetic acid, diethylacetic acid, cyclohexanecarboxylic acid, cyclopentylpropionic acid, cyclohexylbutyric acid, cyclohexylpropionic acid, undecylenic acid, benzoic acid, phenylacetic acid, phenylpropionic acid, phenylbutyric acid, phenoxyacetic acid, acetylacetic acid, malonic acid, succinic acid, glutaric acid, pimelic acid and tartaric acid. The new compounds can be prepared in accordance with methods which are in themselves obvious. Usually, the starting materials are the corresponding ll-methylene-oestr-4-en-17-ones which are described in NL-A-7,216,767, and into these the double bond between C-15 and C-16 is introduced, after which the substituents in positions 3 and/or 17, if these are desired and not yet present, are introduced. The double bond between C-15 and C-16 can be obtained by first introducing a 15-hydroxyl group microbiologically into an 11-methylene-oestr-4-en-17-one compound and then carrying out a dehydration reaction. A 15-hydroxylation can for example be effected with fungi of the genus Penicillium, for example P. raistrickii or P. patulum, or with species of the genera Colletotrichum, Giberella or Glomerella, for example Colletotrichum antirrhini, Giberella baccata or Glomerella cingulata. The dehydration is advantageously carried out by converting the 15a-hydroxyl group to the acylate or sulphonate, after which the 15a-acyloxy or 15a-sulphonyloxy group is split off, with formation of the double bond between C-15 and C-ló. Suitable acyl groups are acetyl, trifluoroacetyl, propionyl, butyryl, heptanoyl and benzoyl. Suitable sulphonyl groups are mesyl, ethanesulphonyl, propionylsulphonyl and p-tosyl. The esterification of the 15a-hydroxyl group is carried out in the usual manner by reacting with an acid, an acid anhydride or an acid chloride, in the presence of a strong acid, such as p-toluenesulphonic acid, or in the presence of a base, for example a tertiary amine, such as pyridine or 4-(dimethylamino)pyridine, if desired at an elevated temperature. The sulphonylation is usually carried out with the appropriate sulphonyl chloride in an anhydrous medium, for example in dry pyridine, with the aid of dimethylformamide and sodium acetate. The introduction of the substituent R2 can be effected in the customary manner by alkylating the 17-oxo compound with an organometallic ethinyl, vinyl, chloroethinyl, allyl or propinyl compound. Examples of such organo-metallic compounds are potassium acetylide, vinyl-magnesium bromide, lithium chioroacetylide, allylmagnesium bromide and potassium methylacetylide. The organo-metallic compound can also be formed in situ, after which it is reacted with the 17-ketone. A 17 -vinyl compound can also be obtained by partial reduction of a 17a-ethinyl compound, for example with the aid of hydrogen in the presence of a catalyst, such as nickel, platinum or palladium on bariumsulphate. During the introduction of the double bond between C-15 and C-16 a 3-oxo group which may be present is temporarily protected, in the customary manner, in the form of an acetal thereof, for example the acetal derived from ethylene glycol, ethanedithiol or 2,2-dimethyl 1, 3-propanediol. The cleavage of the acetal (in the case of a thioacetal, after activation) can be effected with acids, for example with sulphuric acid, perchloric acid, hydrochloric acid or oxalic acid, preferably in alcoholic solution or in acetone, if desired at an elevated temperature. A 3-dithioethylenedithioacetal group which may be present can also be cleaved reductively, for example with sodium in ammonia or with lithium in methylamine, thereby giving a compound where Rg t H2. in this reduction, a 176-substituent having a triple bond, if present, is reduced to a substituent with a double bond, for example, 17a-ethinyl becomes 17(L-vinyl. For the preparation of the 3-desoxo-17-ethinyl compound it is also possible to start from a 3-desoxo-1 1-methylene-1 7-ketone. Esterification of the 178-hydroxyl group, if desired, can be effected in the customary manner, as described above for the 1Sb-hydroxyl group. The new compounds according to the invention, especially ll-methylene-17 -ethinyl-17ss-hydroxy-oestra-4,15-dien-3-one, possess improved ovulation-inhibiting properties upon oral administration in comparison with the 18-methyl analogues, described in EP-A-0,051,762. Further the new compounds are less androgenic than the corresponding ll-methylene compounds without a double bond between C-15 and C-16, described in NL-A-7,216,767. In particular, receptor binding studies with human cell lines show that ll-methylene-17a-ethinyl-17ss- hydroxy-oestra-4,15-dien-3-one has a more advantageous profile than the corresponding known compounds. The very low affinity to SHBG (steroid hormone binding globulin) is also striking. It is believed that the lower affinity to SHBG reduces the metabolic burden of the liver. Compared to the known 18-methyl compounds, they furthermore have the advantage that they are simpler to prepare. 18-Methyl-steroids are usually prepared by total synthesis. This complicated method of preparation, whereby racemates are produced, is not necessary for the preparation of the new compounds. Methods of preparation of 18-methylsteroids, wherein the 18-methyl group is introduced into 13-methylsteroids, are also known, see, for example, NL-A-7,409,512 and NL-A-7,411,607. These reactions, too, need not be used in the preparation of the new steroids according to the invention The compounds according to the general formula I can, usually after having been mixed with auxiliaries and, if desired, with other active constituents, be administered parenterally or enterally, in the form of solutions, suspensions, emulsions or solid pharmaceutical mouldings, such as tablets, pills and dragees. They are especially suitable for use in contraceptive preparations, either by themselves or in combination with an oestragen, such as ethinyloestradiol, or an oestradiol-178-ester, for example oestradiol-17B-valerate, oestradiol-1 7-decanoate or oestradiol-17ss-cyclooctylacetate. For oral administration there are used, for example, tablets, containing 0.02-0.4 mg of a compound according to the general formula I and 0.02-0.05 mg of ethinyloestradiol (or an equivalent amount in respect of effect) of oestradiol-176-ester), of which, for example, one tablet per day is taken. The examples which follow illustrate the invention. Example 1 a) ll-Methylene-15 -hydroxy-oestr-4-ene-3,17-dione 50 ml of a medium consisting of a mixture of glucose (10 gtl) and yeast extract (10 gil) were introduced into a 250 ml shaken Erlenmeyer flask. The medium was inoculated with spores of Gomerella cingulata (ATCC 10534). It was then incubated for 2 days at 28 "C, with shaking. The preculture thus obtained was used to inoculate 2 litres of medium (containing 40 gil of glucose and 10 g/l of yeast extract) in a 5 litre fermenter). The mixture was incubated with stirring (750 rpm) at 28 "C at pH 5.0 for 16 hours, while passing air (0.2 litre/litre of mediumXminute) through it. Thereafter, ll-methylene-oestr-4-ene-3,17dione (0.6 g), suspended in 40 ml of Tween (R) 80 (10%), was added. After 25 hours, the reaction which was extracted with a 9/1 methylene chloridelmethanol mixture. The extract was evaporated to dryness and the residue was purified by chromatography over silica gel and crystallization from methanol. 0.26 g of 11-methylene-15Ci-hydroxy-oestr-4-ene-3,17-dione was obtained, melting point 237 "C and l J2 +294 (chloroform, c - 1%). b) ll-Hethylene-oestra-4,15-diene-3,17-dione-3-ethylene-díthioacetal 10.6 ml of ethanedithiol and 4.6 ml of boron trifluoride etherate were added successively, at 0-5 "C, to a cooled suspension of 21.3 g of 11-methylene-15-hydroxy-oestr-4-ene-3,17-dione in 320 ml of methanol. After the reaction mixture had been stirred for 6 hours at 0-5 "C, it was poured out into 4.5 litres of water and the precipitate was filtered off, washed neutral with water and dried in vacuo. The 26.7 g of crude 11-methylene-15&alpha;-hydroxy-oestr-4-ene-3,17- eM-II (b dione-3-ethylenedithioacetal, thus obtained, were employed iwthe next step without further purification. An analytical sample was obtained @20 after crystallisation from acetonitrile. Melting point 228 "C; [&alpha;]D20 +240 (in chloroform, c w 1X). The crude product was dissolved in 172 ml of dry pyridine. 34.3 ml of methanesulphonyl chloride were added dropwise to this solution at 0-5 ssC over three quarters of an hour, with)good' stirring and in a nitrogen atmosphere. After 1.5 hours of stirring at 0-5 C, r 139.5 ml of dry dimethylformamid and 82.5 gof amhydrous ydrÏús sodium acetate were added. After this reaction mixture had been stirred for 5.5 hours at room temperature, it was poured out into 5 litres of water. The precipitate was filtered off, thoroughly washed with water and dried in vacuo. After purification by chromatography over silica gel followed by crystallisation from methylene dichloride/ acetonitrile, 17.4 g of 11-methylene-oestra-4,15-diene-3,17-dione-3- ethylene-dithioacetal were obtained; melting point 207.5 C;[&alpha;]D20 +142 (chloroform; c - 1%). c) 11-Methylene-17&alpha;-ethinyl-17ss-hydroxy-oestra-4,15-diene-3-one- 3-ethylenedithioacetal A solution of 3.5 g of 11-methylene-oestr-4,15-diene-3,17-dione- 3-ethylenedithioacetal in 52.5 ml of dry tetrahydrofuran was added, at -15 "C to -20 "C, with stirring, to a tetrahydrofuran solution of potassium acetalide, prepared from 8.4 g of potassium tert.-butylate in acetylene. The reaction mixture was stirred for a further hour at this temperature, with continuous passage of acetylene. Thereafter, a mixture of 10 ml of water and 10 ml of tetrahydrofuran was added dropwise to the reaction mixture at -15 0C to -20 "C. The reaction mixture was then poured out into a mixture of 1 litre of saturated NaCl solution and 0.5 litre of water. The precipitate was filtered off, washed neutral with water and dried in vacuo. After purification by chromatography over silica gel and crystallisation from diethyl ether/hexane, 2,6 g of 11-methylene-17&alpha;-ethinyl-17ss-hydroxy-oestra- 4,15-diene-3-one-3-ethylenedithioacetal were obtained, melting point 20 170.5 "C; (&alpha;]D20 - -21 (chloroform, c = 1%). d) 11-Methylene-17&alpha;-ethenyl-17ss-hydroxy-oestra-4,15-diene-3-one 1.41 g of potassium carbonate and 8.1 ml of methyl iodide were ,added successively to a suspension of 3 g of the dithioacetal of Example Ic) in a mixture of 76 ml of methanol and 5 ml of water. The reaction mixture was boiled for 20 hours under reflux, evaporated in vacuo to about 25 ml, further diluted with 250 ml of water and extracted with methylene chloride. The crude product was purified by chromatography over silica gel. Crystallisation of the pure fractions from hexane gave 1.S g of 11-methylene-17&alpha;-ethinyl-17-hydroxy- oestra-4,15-diene-3-one; melting point 157 "C, 20 -44 (chlorofor, c - 1%). Example II a) 11-Methylene-oestra-4,15-diene-3,17-dione-3-ethylene-dithio- acetal-1 7-ethylenediacetal A solution of 15 g of 11-methylene-oestra-4,15-diene-3,17- dione-3-ethylenedithioacetal and 0.5 g p-toluenesulphonic acid in 160 ml of methylene chloride, 320 ml of ethylene glycol and 48 ml of triethylorthoformate was boiled for 4 hours under a reflux condenser. Working up of the reaction mixture by extraction gave 16.9 g of l ethylene-oestra-4,15-diene-3,17-dione-3-ethylenedithioacetal- 17-ethylenediacetal. b) 11-Methylene-oestra-4,15=dien-17-one-17-ethylenediacetal A solution of 14 g of 11-methylene-oestra-4,15-diene-3,17- dione-3-ethylenedithioacetal-1 7-ethylenediacetal was added dropwise to a solution of 7.2 g of sodium in 280 ml of liquid ammonia in 30 minutes at -40 OC, after which the mixture was stirred for a further 30 minutes at the same temperature. After working up and chromatography over silica gel, 9.2 g of 11-aethylene-oestra- 4, 15-dien-17-one-17-ethylenediacetal were obtained. c) ll-Methylene-oestra-4 15-dien-17-one A solution of 8.8 g of 11-methylene-oestra-4,15-dien-17- one-17-ethylenediacetal in 175 ml of acetone and 0.9 ml of concentrated HC1 was stirred for 1.5 hours at room temperature under N2. Working up by extraction and crystallisation from ether gave 6.5 g of 11-methylene-oestra-4,15-dien-17-one. d) ll-Methylene-17d-ethinyl-oestra-4,15-dien-17ss-ol 6 g of ll-methylene-oestra-4,15-dien-17-one were ethinylated in the manner described in Example Ic), giving 2.1 g of ll-methylene- 17d-ethinyl-oestra-4,15-dien-17ss-ol. Example III 11-Methylene-17 -ethinyl-oestra-4,15-dien-17B-ol Following the procedure described in Example I and starting from ll-methylene-oestr-4-en-17-one, 11-methylene-17X-ethinyl-oestra-4,15- dien-17ss-ol was prepared, it being possible to omit the protection and deprotection of the 3-oxo group. Example IV ll-Methylene-l7d-allyl-l7ss-hydroxy-oestra-4l5-dien-3-one On repeating Example I with the difference that alkylation was carried out with allyl-magnesium bromide in Example Ic), ll-methylene- 17 -allyl-17ss-hydroxy-oestra-4,15-dien-3-one was obtained. Example V ll-Methylene-17d propinyl)-17ss-hydroxy-oestra-4,15-dien-3-one On repeating Example I with the difference that in Example Ic) alkylation was carried out with methylacetylene in the presence of butyl-lithium, 11-methylene-17f-{1-propinyl)-17ss-hydroxy-oestra-4,15- dien-3-one was obtained. Example VI 11-Methylene-17&alpha;-chloroethinyl-17ss-hydroxy-oestra-4,15-dien-3-one On repeating Example I with the difference that in Example Ic) alkylation was carried out with 1,2-diehloroethylene in dimethyl ether tetrahydrofuran (8:1), in the presence of methyl-lithium, 11-methylene-17&alpha;-chloroethinyl-17ss-hydroxy-oestra-4,15-dien-3-one was obtained. Example VII 11-Methylene-17 -ethinyl-17ss-hydroxy-oestra-4.15-dien-3-one- 17B-acetate 1.8 g of 11-methylene-17&alpha;-ethinyl-17ss-hydroxy-oestra-4,15- dien-3-one were reacted at room temperature with 10 ml of acetic anhydride in 15 ml of pyridine in the presence of 150 mg of 4-dimethylaminopyridine. After 5 hours, the mixture was poured out into ice water and worked up. Chromatography of the product over silica gel, with acetone/hexane, gave 1.6 g of 11-methylene-17 - ethinyl-17ss-hydroxy-oestra-4,15-dien-3-one-17ss-acetate. The 17ss-esters derived from butyric acid, valeric acid and decylic acid were prepared analogously. Example VIII Measurements of binding affinities of 11-methylene-176- ethinyl-17ss-hydroxy-oestra-4,15-dien-3-one and the analogues with 18-methyl or without the double bond at position 15 to the progesteron-, the androgen- and the SHBG receptor revealed the following: EMI11.1 <tb> <SEP> compound <SEP> receptor <SEP> affinity <SEP> to <tb> #13 <SEP> <SEP> 18-CH3 <SEP> progesteron <SEP> receptor <SEP> androgen <SEP> receptor <SEP> SHBG <SEP> receptor <tb> <SEP> (3-keto <SEP> desogestrel <SEP> (5&alpha;-dihydro <SEP> <SEP> (5&alpha;-DHT <SEP> <SEP> - <SEP> 100) <tb> <SEP> - <SEP> 100) <SEP> testosterone <SEP> 1 <SEP> 100 <tb> <SEP> . <SEP> <tb> <SEP> - <SEP> - <SEP> 80 <SEP> 7 <SEP> 3 <tb> <SEP> + <SEP> + <SEP> 99 <SEP> 2 <SEP> 5 <tb>

Claims: CLAIMS 1. New 11-methylene-steroids of the general formula I: EMI12.1 (formula I) wherein R1 * H or acyl with 1-18 C-atoms, R2 - ethinyl, vinyl, chloroethinyl, allyl or propinyl, and R3 " O or H2. 2. Compounds according to claim 1, characterized in that R1 - H. 3. Compounds according to claims 1-2, characterized in that R2 ethinyl. 4. Compounds according to claims 1-3, characterized in that R3 - 0. 5. 11-Methylene-176-ethinyl-17ss-hydroxy-oestra-4,15-dien-3-one. 6 Process for the preparation of the steroids according to claims 1-5, characterised in that an 11-methylene-oestra-4-en-17-one compound is used as the starting material, a 15h-hydroxy group is introduced into it, thereafter the product is dehydrated to give a double bond between C-15 and C-16 and finally the substituents desired in position 3 and/or 17 are introduced in a manner known per se. 7. Pharmaceutical preparations comprising one or more of the steroids according to claims 1-5.

Inventors: BERGINK, ENGELBERT WILLEM, DR.
Applicants: AKZO N.V.
Application year: 1986
Main classification: C07J1
IPC codes: C07J1, C07J33, A61K31, C07J9, C12P7, C07J21, C09J7, C07J11, C12R1