PROCESS FOR THE PREPARATION OF QUINOLINE CARBOXYLIC ACIDS

Abstract: New process for the preparation of compounds of general Formula (I), (wherein R stands for hydrogen or methyl) and pharmaceutically acceptable salts thereof which comprises reacting a compound of general Formula (V), (wherein R<1> and R<2> stand for an aliphatic acyloxy group comprising 2-6 carbon atoms and optionally substituted by halogen; or for an aromatic acyloxy group comprising 7-11 carbon atoms) with an amine of general Formula (VI), (wherein R has the same meaning as stated above) or a salt thereof and subjecting the compound of general Formula (VII), thus obtained (wherein R, R<1> and R<2> are as stated above) to hydrolysis after of without isolation and if desired converting the compound of general Formula (I) thus obtained into a salt thereof or setting free the same from its salt. The compounds of general Formula (I) are known antibacterial agents. The advantage of the process of the present invention is that it makes the desired compounds of general Formula (I) available in a simple manner, with high yields and in a short reaction time.

Description: PROCESS FOR THE PREPARATION OP QUIl.OLDTΞ CARBOXYLIC ACIDS This invention relates to a new process for the preparation of l-ethyl-6-JTl oii-7*-(4-optionally sub¬ stituted pi erazino)-4-oxo-l,4-dihydro-quinoline-3- -carboxylic acid derivatives and pharmaceutically acceptable salts thereof. It is known that the quinoline carboxylic acid derivatives of the general Formula I are widespreadly used due to their high antibacterial activity for the treatment of diseases of the urinary tract "and those of systemic bacterial origin (J. Pharm. Sci. 1984, 73, 1379; Eur-. J. Che not her. Antibiot. 1983, 3- pages 9 and 47; J. Antimicrob. Che mother. 1984, 13, Suppl., _99, pages 107 and 113). In the general Formula I R stands for hydrogen or methyl. The quinoline-carboxylic acid of the general Formu¬ la I (wherein R stands for hydrogen) can be prepared by reacting l-ethyl-6-f luoro-7-chloro-4-oxo-l,4-dihydro- -quinoline-3-carboxylic acid and piperazine (Belgian patent s ecifications _Tos. 363,429 and 370,576; Japane¬ se patent s ecification No. 8033,543 and J. Med. Che . 23, 1358 /1980/) or by subjecting ethyl-l-ethyl-6-f luoro* -7- piperazino -4 -oxo -1,4-dihydro-quino line -3 -carboxy late of the Formula III to hydrolysis (Belgian patent specifications Nos. 879,106 and 890,223). The common disadvantage of the above processes is that the exchange reaction of the chlorine atom in po¬ sition 7 to piperazine is carried out under vigorous reaction conditions, at a temperature of 115-175 C, for 5-19 hours, optionally even under pressure. More¬ over the reaction is not regioselective and under the above reaction conditions the fluorine atom in posi¬ tion 6 partially reacts with piperazine (J. Med. Chem. 23, 1358 /1980/). The quinoline carboxylic acid of the general For¬ mula I, wherein R stands for methyl, cay be prepared by reacting l-ethyl-6-fluoro-7-chloro-4-oxo-l,4-dihydro- -quinoline-3-carboxylic acid of the Formula II with 1-me thyl-piperazine (Belgian patents NOs . 370 , 576 and 370, 917 ; Laid-open Japanese patent application No . 80 33 , 453 and J . Med. Che m. 2 , 1358 /1980/ ) or by ethylating l-e thyl-6-f 1UO_D -7 -pi perazino-4 -oxo -l, 4- -dihydro-quinoline-3-carboxylic acid of the Formula IV (Belgian patent No. 870,917 and FR-OS No. 2,424,919). The common disadvantage of the above processes is that the reactions are carried out under vigorous and agressive reaction conditions, at a temperature bet¬ ween 110 and 150 °C, during 7-16 hours. The yields vary between 55 % and 66 %. Moreover the reaction mix¬ ture is worked up in a complicated manner. According to the present invention there is provi¬ ded a new process for the preparation of compounds of the general Formula I (wherein R stands for hydrogen or methyl) and pharmaceutically acceptable salts there¬ of, which comprises reacting a compound of the general Formula V (wherein R1 and R2 stand for an aliphatic acyloxy g oup comprising 2-6 carbon atoms and optionally substituted by halogen; or for an aromatic acyloxy group comprising 7-11 carbon atoms) with an amine of the general Formula (wherein R has the same meaning as stated above ) or a salt thereof and subjecting the compound of the general Formula VII thus obtained (wherein R, R 1 and R2 are as stated above) to hydrolysis after or without isolation and if desired converting the compound of the general Formula I thus obtained into a salt thereof or setting free the same f om its sal" . The advantage of the process of the present inven¬ tion is that it enables the preparation of the compounds of the general Formula I in a simple manner, with very high yields and in a short reaction time. In the general Formula V 1 and R2 may be the same or different. The borate derivatives of the general Formulae V and VII are new compounds. According to a preferred form of realization of the process of the present invention the borate derivative of the general Formula VII is converted into the desired quinoline-3-carboxylic acid of the general Formula I without isolation. The borate derivatives of the general Formula V can be reacted with the amine of the general Formula VI if desired in the presence of an inert organic solvent and an acid binding agent. As inert organic solvent preferably an acid amide (e.g. dimethyl forma ide, dimethyl aceta ide), a ketone (e.g. acetone, methyl ethyl ketone), an ether ( e.g. dioxane, tetrahydrofuran, diethyl ether), an ester (e.g. ethyl acetate, methyl acetate, ethyl propionate), a s Ifoxide (e.g. dimethyl sulfoxide), an alcohol (e.g. methanol, ethanol, 1-decanol, butanol) may be used. As acid binding agent an organic or inorganic base may be used. From the group of organic bases trialkyl amines (e.g. triethy1 amine, tributyl amine), cyclic amines (e.g. pyridine, 1,5-diazabicyclo/5-4.0/undec-5- -ene, 1,5-diazabicyclo/4.3•0/non-5-ene, 1,4-diazabicyclo- /2.2. /octane) can be mentioned, while as inorganic base preferably hydroxides or carbonates of alkali or alka¬ line earth metals can be applied. Thus as acid binding agent advantageously potassium carbonate, potassium hydrogen carbonate, sodium hydroxide, calcium hydroxide, etc. or an excess of the amine of the general Formula VI can be used. The borone derivative of the general Formula V and the amine of the general Formula VI can be reacted at a temperature between 0 and 200 °C, depending on the solvent used. The reaction time may vary between hal an hour and 10 hours. The reaction time depends on the reaction temperature, too. If the reaction is carried out at higher temperature, the reaction time can be shortened. The above reaction conditions are but preferab values and other conditions may be used as well. The borates of the general Formula VII can be hydro- lyseά to the desired quinoline-3-carboxylic acids of the general Formula I, after or without isolation, under acidic or basic conditions. The compound of the general Formula VTI precipitates from the reaction mixture e.g. on cooling and can be separated e.g. by filtration or centrifuging, if desired. Basic hydrolysis may be preferably carried out with the aid of a hydroxide or carbonate of an alkali metal or an alkaline earth metal hydroxide, used as aqueous solution. One may preferably use an aqueous solution of sodium hydroxide, potassium hydroxide, sodium carbonate, potassium hydrogen carbonate, potassium carbonate, calcium hydroxide. However, organic amines (e.g. triethy amine) may also be applied in the hydrolysis step. Acidic hydrolysis may preferably be accomplished by using an aqueous mineral acid-. One cay preferably proceed by hydrolysing a borate of the general Formula VII by heating with an aqueous solution of hydrochlo¬ ric acid, hydrogen bromide, sulfuric acid or phosphoric acid. Hydrolysis may also be accomplished with the aid of an organic acid (e.g. acetic acid, propionic acid, etc. ) . Hydrolysis of the compounds of the general Formula VII may also be carried out in aqueous medium in the presence of a water- iscible organic solvent. For this purpose e.g. alcohols (e.g. methanol, et anol), a ketone (e.g. acetone), an ether (e.g. dioxane ), an acid amide (e.g. dimethyl formamide), a sulfoxide (e.g. dimethyl sulfoxide), or pyridine may be used. The quinoline-3-carboxylic acid of the general For¬ mula I thus obtained may be isolated e.g. by adjusting the pH value of the aqueous solution to a suitable value and separating the precipitated crystals e.g. by filt¬ ration or centrifuging by liophylizing the aqueous reac¬ tion mixture. The compounds of the general Formula I can be con- verted into pharmaceutically acceptable salts thereof in a known manner. Thus preferably acid addition salts can be formed, e.g. salts formed with hydrogen halides, sulfonic acids, sulfuric acid or organic acids. One may form preferably the chlorides, bromides, aryl sul- fonates, methane sulfonates, aleates, fu arates, ben- zoates, etc. The compounds of the general Formula I form salts with alkali or alkaline earth metals or other metal ions as well. Accordingly the sodium, potassium, magnesium, iron, copper salts, etc. may be prepared. The compounds of the general Formula I and pharma¬ ceutically acceptable salts thereof can be converted into the hydrates (e.g. hemihydrates, trihydrates, etc.) by methods known per se. According to a further aspect of the present in¬ vention there are provided new compounds of the general Formula VII (wherein R, R 1 and R2 are as stated above).. The starting materials of the general Formula V can be prepared by reacting l-ethyl-6-fluoro-7-chloro- -4-oxo-l,4-dihydro-quinoline-3-carboxiiatic acid of the Formula II (Belgian patent No. 863,429) with a boron derivative ( e.g. with a compound of the general For¬ mula vTII /wherein R 3, R4 and R5 stand for an alkyl group having 1-5 carbon atoms and optionally substituted by .halogen or an aryl group comprising 6-10 carbon atoms/) optio¬ nally in an organic medium. Further details of the present invention are to be found in the following Examples without limiting the _. - sc pe of protection to the said Examples. Example 1 19.5 g of l-ethyl-6-fluoro-7-chloro- -oxo-1,4-di- hydro-quinoline-3-carboxylic acid—borone-di(propionyl- oxy)-anhydride and 11.9 g of piperazine are reacted in 72 ml of dimethyl sulfoxide at 110 °C for an hour. The reaction mixture is cooled to 90 °C and 116 ml of a 6 weight/vol % aqueous sodium hydroxide solution are added. The aqueous reaction mixture is kept for an hour under slight boiling whereupon it is cooled to room temperature. The pH value of the solution is adjusted to 7 with 96 weight/vol % acetic acid. The reaction mix¬ ture is allowed to crystallize in a refrigerator over¬ night. Next morning the precipitated crystals are fil¬ tered, washed with water and dried in vacuo at 90-95 °C until constant weight. Thus 14.0 g of l-ethyl-6-fluoro- -4-oxo-l,4-dihydro-7-piperazino-quinoline-3-carboxylie acid are obtained, yield 95.9 % - The product decomposes at 221-222 °C (from a mixture of dichloro methane and methanoD Analysis for the Formula C-,.-H-,pF Oo calculated C = 60.18 ; " ., ~H a ° 5.68 £, IT = 13.16 5 found C = 60.07 .J, H = 5-74 %- N = 13.18 T The starting material is prepared as follows: A mixture of 9.3 g of boric acid and 70 g of prop- ' ionic anhydride is stirred at 100 °C for 15 minutes, whereupon the reaction mixture is heated to the boiling point. After half an hour the temperature is lowered to 110 °c and 29.8 g of ethyl-l-ethyl-6-fluoro-7-chloro- -4-oxo-l,4-dihydro-quinoline-3-carboxylate are added. The reaction mixture, which turns into a thick suspen¬ sion within some minutes, is stirred at 110 °C for 2 hours, cooled .to room temperature and diluted with 300 ml of water. The reaction mixture is coo led and the pre¬ cipitated crystals are filtered . Thus 41. 5 g of 1-e thyl- -6-f luoro-7-chlor 0-4-oxo- 1, 4-dihydr o-quinoline -3-carbo- xylic acid-borone -di ( propionyloxy ) -anhydride are obtaine yie ld 97.7 %. The product decomposes at 252 °C. Analysis for the Formula ' C^H^BFClNOγ calcu lated C = 50.79 % - H = 4.26 %, N = 4.29 % . found C = 50. 94 %, H = 4. 15 %, N = 3.41 %. Example 2 19.5 g of l-ethyl-6-fluoro-7-chloro-4-oxo-l,4-di- hydro-quinoline-3-carboxylic acid-borone-di(propionyloxy) -anhydride and 13.3 g of 4-methyl-piperazine arereacted in 72 ml of dimethyl sulfoxide at 110 °C for 2 hours. The reaction mixture is cooled to 90 °C and 116 ml of a 6 weight/vol % aqueous sodium hydroxide solution are added. The aqueous reaction mixture Is kept for an hour under slight boiling and cooled to room temperature. The pH value of the solution is adjusted to 7 with a 6 weight/vol % acetic acid. The reaction mixture is allowed to crystallize in a refrigerator, after 5 hours the- precipitated crystals are filtered, washed wit water and dried at 85 C in vacuo until constant weight. Thus 13-9 g of l-ethyl-6-fluoro-7-(4-methyl-piperazino)-4- -oxo-1, -dihydro-quinoline-3-carboxylic acid are obtaine yield 269-271 °C. (The product decomposes at 269-271 °C from a mi-Xture of dimethyl forniamide and ethanol.) Analysis for the Formula C.-H pQ F ^O--, calculated C = 61.25 % - H = 6.05 3 N = 12.6θ found C = 61.37 % - H = 5-91 % - N = 12.47 2 g of l-ethyl-6-fluorc-7-(4-methyl-piperazino)-4- -oxo-1,4-dihydro-quinoline-3-carboxylic acid are heated in 30 ml of anhydrous ethanol to boiling and to the boiling solution 0.61 g of ethanesulfonic acid are adde From the so lution thus obtained the precipitation of crystals begins within some minutes . After 10 minutes the reaction mixture is cooled to 0 °C and allowed to crystallize in a refrigerator overnight . Next day the precipitated crystals are filtered, washed with ethanol and dried in vacuo at 90-95 °C until constant weight. Thus 2.3 g of the ethanesulfonic acid salt of l-ethyl-6-f uoro-7-(4-methyl-l-piperazino ) -4-oxo- -l, 4-dihydro-quinoline-3-carboxylic acid are obtained , yie ld 89.3 %- The product decomposes at 285-287 °C. Analysis for t calculated C %, TS - 9.78 %% found C a 50. 12 %, H = 5.81 %, IT = 9.79 %. The starting material may be pre pared as follows : A mixture of 9.3 g of boric acid and 70 g of propio- nic anhydride is stirred at 100 °C for 15 minutes where¬ upon the temperature is raised to the boiling point . After half an hour the te mperature is lowered to 110 °C and 29.8 g of e thyl-l-ethyl-6-f luoro-7-chlorc-4-oxo-l, 4- -dihydro -quino line -3 -carboxylat are added. The reaction mixture , which turns into a thick suspension within some minutes , is heated at 110 °0 for 2 hours, cooled to room temperature and di luted with 300 ml of water. The reac¬ tion mixture is cooled and the precipitated crystals are* filtered. Thus 41. 5 g of l-ethyl-6-fluoro-7-chloro- -4-oxo-l, 4-dihydro-quinoline-3-carboxylic acid-borone- -di ( r 0 pi onyl o y) -anhydride are obtained, yield 97.7 . The product decomposes at 252 °C. Analysis for the Formula C^gH ^ gBFClNOγ calculated C = 50.79 % - H = 4.26 %, N = 3.29 % found C = 5C. 94 % - H = 4. 15 %, 11 = 3.41 ? Example 3 19.9 g of (l-ethyl-6-fluoro-7-chloro-4-oxo- -1 ,4-dihydro-quinoline-3-carboxylate-Q_ 3 ,0_4)-bis(acetate- -0_)-boroπ and 15.0 g of 1-methyl piperazine are reacted in 100 ml dimethylsulfoxide at 110-115 °C for 2 hours. The reaction mixture is then allowed to cool to 80-90 °C and 126 ml of a 6 % by W/V aqueous solution of sodium hydroxide are added. The reaction mixture is mildly boiled for 1.5 hours, whereafter active charcoal is added and filtered. After cooling to room temperature the pH of the solution is adjusted to 6.5 by adding 96 % by W/V acetic acid. The reaction mixture is kept at 0 C for half day, whereafter the precipitated crystals are filtered and washed with water and methaπol. Thus 14,3 g (85.8 H ) of l-ethyl-6-fluoro-7-(4-methyl- -piperaziπo)-4-oxo-l, -dihydro-quinoliπe-3-carbαxylic acid are obtained. Decomposition at 268-270 °C (from a mixture of dimethylformamide and methaπol). No depression of melting point is obtained when admixing the product with the compound obtained according to Example 2. The preparation of the starting material: 9.3 g of boric acid and 54. L g. of acetic acid anhydride (95 -.) are admixed and slowly heated and kept at 100 °C for 30 minutes. 29.8 g of ethyl-(1-ethyl- -6-fluoro-7-chloro-4-oxo-l,4-dihydro-quinoliπe-3- -carboxylate are then added to the reaction mixture. Within some minutes the first crystals are precipitated, the suspension paste is stirred for 2 hours and cooled to 10 °C and 100 ml of cold water are added and the precipitated crystals are filtered. Thus 38.7 g. (97.5 %) of (l-ethyl-6-fluoro-7-chloro-4-oxo-l,4-dihydro- -3-quinoliπe-carboxylate-0_ ,0_ )-bis(acetate-0_)-boron are obtained, decomposing at 278 C. Analysis for the formula (_ 1( -H 14 BClF α 7 calculated: C = 48.35 % H = 3.55 % N = 3.52 H found: C = 48.49 % H = 3.46 H N = 3.71 % . Ex amp le 4 3.97 g. of (l-ethyl-6-fluoro-7-chloro-4-oxo- -1 ,4-dihydro-quinoline-3-carboxylate-0_ ,0_ )-bis- (acetate-0.)-boroπ and 3.00 g. of 1-methyl-piperaziπe are reacted in 40 ml of dimethylsulfoxide for 250 hours at 25 °C. To the reaction mixture 20 ml of an aqueous solution of 6 % by W/V sodium hydroxide are added and the reaction temperature is raised to 110 C within 30 minutes. The mixture is mildly boiled for a further half hour, it is filtered warm and cooled. The pH of the solution is adjusted to 7 by adding 36 by W/V acetic acid. The reaction mixture is allowed to crystallize overnight in a refrigerator and the precipitated crystals are filtered and washed with some water, and beige crystals are obtained (2.8 g, 84 %). After recrystalliza- tioπ from a mixture of dimethylformamide and me ' thaπol l-ethyl-6-fluorα-4-oxo-7-(l-methyl-piperaziπo)-l,4- -dihydro-quiπoline-3-carboxylic acid is obtained, decompos¬ ing at 267-269 °C after drying to constant weight at 90-95 C in vacuo. No melting point depression is obtained when admixed with the product of Example 2 at any ratio. Example 5 7.95 g (l-ethyl-6-fluoro-7-chloro-4-oxo-l ,4- -dihydro-3-quiπoline-carboxylate-0_ 3 ,0_4)-bis(acetate-0_)- -boroπ and 5.17 g. of piperazine are reacted at 110 °C for 1 hour in 30 ml. of dimethylsulfoxide . The reaction mixture is then cooled to 90 C and an aqueous solution of 6 by W/V sodium hydroxide (30. ml.) is added and the mixture is heated to 110 °C within half hour and a further half hour stirred at this temperature. The mixture is filtered hot, then cooled to room temperature and then the pH is adjusted to 6.5 by adding 96 % (-,y . W/V acetic acid. The product is allowed to stand for 1 day and the precipitated crystals are filtered and washed with water. l-Ethyl-6-fluαro-7-piperaziπo-4- -oxo-1 , 4-dihydro-quiπoliπe-3-3-carboxylic acid is obtain¬ ed (4.2 g, 66 %), which does not show any depression of melting point when admixed with the product of Example 1 Example 6 3.97 g. (l-ethyl-6-fluoro-7-chloro-4-oxo-l ,4- -dihydro-quinoline-3-carboxylate-O i ,0. ) -bis(acetate-0_)- -boron are reacted with 2.58 g. of piperazine in 20 ml of dimethylsulfoxide for 180 hours at 25 Q C. To the reaction mixture an aqueous solution of 6 % by W/V sodium hydroxide (20 ml) is added and the reaction temperature is raised to 110 C within a half hour. The mixture is then mildly boiled for another 30 minutes, filtered hot and cooled. The pH of the solution is adjusted to 7 with 96 % by W/V acetic acid. The reaction mixture is allowed to crystallize " overnight in a refrigerator and the precipitated crystals are filtered, washed with water and pale beige crystals are obtained (3.0 g, 94 %). After recrystallization form dichloro- methaπe-methaπol l-ethyl-6-fluoro-4-αxo-7-piperaziπo- 1 , -dihydro-quinoline-3-carboxylic acid is obtained, melting at 220-222 C after drying to constant weight at 90-95 C in vacua. No depression -of melting paint is observed when admixing the product at any ratio with a substance prepared according to Example 1. Example 7 2.0 g of (l-ethyl-6-fluoro-7-chloro-4-oxo-l ,4- -dihydro-3-quinoliπe carboxylate-0_ ,0_ )-bis(acetate-0_)- -boron and 1.3 g of piperazine are heated under reflux in 40 ml. of abs. methaπol for 10 days on a waterbath. To the reaction mixture 12 ml. of an aqueous solution of 6 . by W/V sodium hydroxide are added and heated under reflux for 2 hours. After cooling the mixture* to room temperature the pH of the solution is adjusted to 6.5 by adding 96 -. by W/V acetic acid and the precipitated yellow crystals are filtered. The obtained l-ethyl-6-fluoro-4-oxo-7-piperaziπo-l,4-dihydro- -quinoline-3-carboxylic acid (1.55 g. 97 -.) is re- crystallized from a mixture of dichloromethane and methaπol, melting point: 220-222 °C. No depression of the melting point is obtained when admixing the product with the product of Example 1.

Claims: 7,ΕAT WE CLAIM IS; 1. Process for the preparation of compounds of the general Formula I (wherein R stands for hydrogen or methyl) and pharma¬ ceutically acceptable salts thereof which comprises reacting a compound of the general Formula V (wherein R 1 and R2 stand for an aliphatic axyloxy group comprising 2-6 carbon atoms and optionally substituted by halogen; or for an aromatic acyloxy group comprising 7-11 carbon atoms) with an amine of the ^ general Formula VT (wherein R has the same meaning as stated above ) or a salt thereof and subjecting the compound of the ge¬ thus obtained (wherein R, R 1 and R2 are as stated above) to hydrolysis after or without isolation and if desired converting the compound of the general Formula I thus obtained into a salt therecf or- setting free the same from its salt. 2. Process according to Claim 1 which comprises reacting a compound cf the general Formula V with an amine of the general Formula VT in the presence of an organic solvent, preferably an acid amide, sulfoxide, ketone,alcohol, ether or ester. 3. Process according to Claim 2 which comprises using dimethyl sulfoxide as organic solvent. 4. Process according to Claim 1 which comprises carrying out the reaction of the compounds of the ge¬ neral Formulae V and VI in the presence of an acid binding agent. 5. Process according to Claim 4 which comprises using an amine or an excess of the compound of the ge- ' neral Formula VI as acid binding agent. 6. Process according to Claim 1 which comprises carrying out hydrolysis in acidic medium. 7. Process according to Claim 6 which comprises carrying out the reaction by using an organic or inor¬ ganic acid, preferably hydrochloric acid, sulfuric acid or acetic acid. 8. Process according to Claim 1 which comprises carrying out hydrolysis in alkaline medium. 9. Process according to Claim 8 which comprises using an alkali metal hydroxide, an alkaline earth metal hydroxide or an organic base, preferably an aqueous triethyl amine solution. 10. - Compounds of the general F 0 rmula VII 2. H*15 (whe-rein R1 and R2 stand for an aliphatic acyloxy group comprising 2-6 carbon atoms optionally substituted by halogen; or an aromatic acyloxy group comprising 7-11 carbon atoms and R represents hydrogen or methyl). 11. Compounds of the general Formula I whenever pre¬ pared according to the process according to any of Claims 1-9. 12. A process as substantially described herein with particular reference to the Examples.

Inventors: BALOGH MARIA, HERMECZ ISTVAN, HORVATH AGNES, KERESZTURI GEZA, KOVACS GABOR, PAJOR ANIKO, RITLI PETER, SIPOS JUDIT, SZUETS TAMAS, VASVARI LELLE, BALOGH, MARIA, HERMECZ, ISTVAN, HORVATH, AGNES, KERESZTURI, GEZA, KOVACS, GABOR, PAJOR, ANIKO, RITLI, PETER, SIPOS, JUDIT, SZUETS, TAMAS, VASVARI, LELLE
Applicants: CHINOIN GYOGYSZER ES VEGYESZET, CHINOIN GYOGYSZER ES VEGYESZETI TERMEKEK GYARA RT.
Application year: 1986
Main classification: C07D215
IPC codes: A61K31, A61P31, C07D215, C07D295, C07D401, C07F5