NON-TOXIC OPHTHALMIC PREPARATIONS AND METHODS OF PREPARING THEM

Abstract: An ophthalmic preparation having an enhanced ability to permit essentially normal maintenance of ocular surface mucus-containing goblet cells while present in the eye, com­ prises a solution, ointment or gel generally having a pH of 7.0 to 8.0 and optionally being isotonic, the preparation including sodium and chloride as major constituents and lesser amounts of potassium and bicarbonate; optionally, the pre­ paration includes one or more of calcium, magnesium phos­ phate.

Description: This invention relates to ophthalmic preparations for application to the ocular surface and methods of making the preparations. More particularly, the invention relates to ophthalmic preparations that permit essentially normal maintenance of ocular surface mucus-containing goblet cells. The conjunctival epithelium of the eye contains mucus-containing goblet cells. These cells secrete mucus in a controlled fashion. They thereby form a major element of the biological system by which the surface of the eye protects itself from harmful foreign matter, including but not limited to infectious agents. The goblet cells also form a critical layer of the tear film of the eye that protects the surface of the eye from dessication. Furthermore, the mucus contained within these goblet cells is a major natural lubricant for the ocular surface. The presence of a normal density of goblet cells is important for the maintenance of a healthy ocular surface. Medicamentosa is a disease of the surface of the eye caused by the frequent administration to the eye of topical medications and/or solutions. The disease can cause ocular irritation, photophobia, conjunctival infection and keratitis. The cause of this disease has been unclear. It now has been found, however, that exposing the surface of the eye to standard ophthlamic solutions results in an accelerated loss of mucus from the conjunctival goblet cells. This loss of mucus leaves the ocular surface with an abnormally low density of mucus-containing goblet cells. This abnormal loss of goblet cell mucus is a manifestation of toxicity of the solution that was used. It is now postulated that one mechanism responsible for the development of medicamentosa is the gradual, progressive depletion of goblet cell mucus that the exposure to standard ophthlamic solutions induces. Derangement of goblet cell mucus can decrease the eye's ability to rid itself of harmful foreign material, can engender dessication of the ocular surface, and can decrease lubrication of the surface. For example, solutions designed to lubricate the eye in fact work against that intention when they deplete the eye of goblet cell mucus --a most important natural lubricant. It is desirable to minimize the toxicity of ophthalmic preparations. What is needed is an essentially non-toxic ophthalmic preparation which satisfies the fluid and electrolyte requirements of the ocular surface. The composition of tear fluid has been determined to include proteins, enzymes, lipids, metabolites and electrolytes. The proteins include tear albumin, lysozyme, lactoferrin, transferrin, caeruloplasmin, immunoglobulins, complement, glycoproteins and antiproteinases. The enzymes include glycolytic enzymes and enzymes of the tricarboxylic acid cycle, as well as lactate dehydrogenase, lysosomal enzymes, amylase, peroxidase, plasminogen activator and collagenase. The lipids include cholestrol, though lipids from other lipid classes are present. The metabolites include glucose, lactate, urea, catecholamines, histamine, and prostaglandins. The electrolytes include sodium, potassium, calcium, magnesium, chloride and bicarbonate. Objects of this invention include the provision of an ophthalmic preparation which permits essentially normal maintenance of ocular surface mucus-containing goblet cells, and which is essentially non-toxic. Desirably, the preparation is suited for the improved treatment of the spectrum of dry eye disorders. Another object is to provide a preparation for selectively increasing or decreasing tear film osmolarity. A preparation according to the invention advantageously is suitable selectively for contact lens adaptation, for use as a vehicle for medicaments, and for eye comfort in general. An ophthalmic preparation according to the invention may serve as an irrigation fluid and/or drug vehicle in patients with extensive lacrimal gland and/or excretory duct destruction and/or other ocular diseases where continuous or nearly-continuous ocular irrigation and/or drug therapy is desirable. A synthetic preparation for ophthalmic use has been developed that has an enhanced ability to permit essentially normal maintenance of ocular surface mucus-containing goblet cells. The preparation leaves the conjunctiva of eyes white and non-swollen, even after prolonged bathing, e.g., for twelve hours. The preparation is non-toxic and contains a combination of electrolytes found in natural tear fluid and satisfies requirements of the ocular surface. In preferred formulations, the synthetic preparation is substantially free of histamines. In other formulations, the preparation is substantially free of immunoglobulins, complement, desquamated epithelial cells, bacteria and/or microorganisms. In general, the preparation consists principally of major amounts of sodium and of chloride, and of lesser amounts of potassium and of bicarbonate. It preferably also contains other naturally-occurring elements. Other constituents may be present depending on the particular purpose of the final preparation, eg. as a lubricating ointment, or as a medicament for the treatment of glaucoma or ocular surface infections. Therapeutic compositions containing electrolytes such as sodium, chloride, potassium and bicarbonate are known, see for example US patent numbers 3,821,368, 3,897,550 and 3,676,553, all in the name of Reynolds. However none of the Reynolds patents concern ophthalmic solutions. Ophthalmic solutions containing electrolytes have been described in US 4,443,432 (Garabedian et al ) and US 3,843,782 (Krezanoski et al ). It has now been found that ophthalmic compositions containing particular quantities of selected electrolytes have a beneficial effect on the normal maintenance of the goblet cells, thereby enabling the abovementioned objects of the present invention to be attained. According to one aspect of the present invention, there is provided a synthetic non-toxic ophthalmic preparation of selected osmolality containing potassium, chloride, bicarbonate, and sodium, said preparation comprising (a) between 22.0 to 43.0, preferably between 23.0 to 42.0, millimoles of potassium per liter, (b) between 29.0 to 50.0, preferably between 31.0 and 48.0 millimoles of bicarbonate per liter, (c) between 130.0 to 140.0, preferably between 131.0 to 139.0, millimoles of sodium per liter, and (d) between 118.0 to 136.5, preferably between 124.0 to 136.0, millimoles of chloride per liter. While the invention is not limited thereby, it is desirable to include calcium, magnesium, and phosphate in the solution. The desirable effects of these elements on living cells are well known. See, for example, the following publications: Edelhauser et al, Am J Ophthalmol , 81:473, 1976; Kaye et al, Invest Ophthalmol Vis Sci , 7:53, 1968; Edelhauser et al, Arch Ophthalmol , 93:648, 1975; Edelhauser et al, Arch Ophthalmol , 96:516, 1978; Merrill et al, Am J Ophthalmol , 49:895, 1960. The preferred concentrations are between 0.5 to 2.0 mMoles/liter of calcium, between 0.3 to 1.1 mMoles/liter of magnesium, and between 0.8 to 2.2 mMoles/liter of phosphate. More optimal is a calcium concentration between 0.6 to 0.8 mMoles/liter, a magnesium concentration between 0.5 to 0.6 mMoles/liter and a phosphate concentration between 1.0 and 2.0 mMoles/liter. The preferred resulting solution has a pH value between 7.0 and 8.0 (as measured with a Fisher pH Accumet Model 600). Nevertheless, this pH range need not be rigidly adhered to, and it may be desirable to alter pH outside of this range, for instance to improve ophthalmic drug penetration through the ocular surface. Those skilled in the art may, in view of the teachings herein, employ other pH ranges. In preferred embodiments the preparation is isotonic. However the final osmolality may be adjusted according to the conditions present in the tear film or on the ocular surface. For example, treatment of hypertonic tear films may make diluted preparations preferable. Alternatively, the preparation may be concentrated to hypertonic concentration when therapeutically desirable such as in topical formulations for the treatment of recurrent corneal erosions. The preparations may be concentrated or diluted while minimizing undesirable properties or untoward effects of final preparation by maintaining the preferred ratios of the described electrolytes of the final ophthalmic preparations. Also according to the invention, there is provided a synthetic non-toxic ophthalmic preparation of selected osmolality containing potassium, chloride, bicarbonate and sodium, the preparation having a ratio of potassium to bicarbonate in the range of 1:0.6 to 1:2.3 mMoles/ liter and a preferred range of 1:.7 to 1:2.1; potassium to sodium in the range of 1:3.0 to 1:6.4 mMoles/liter and a preferred range of 1:3.1 to 1:6.0; and potassium to chloride in the range of 1:2.7 to 1:6.2 mMoles/liter and a preferred ratio of 1:2.9 to 1:5.9. In other formulations according to the invention, the preparation also contains one or more of calcium, magnesium and phosphate ions, when the ratio of potassium to calcium is in the range of 1:0.01 to 1:0.09 mMoles/liter and a preferred ratio is 1:0.01 to 1:0.04; potassium to magnesium is in the range of 1:0.005 to 1:0.05 mMoles/ liter and a preferred ratio is 1:0.01 to 1:0.03; and potassium to phosphate is in the range of 1:0.015 to 1:0.1 mMoles/liter and a preferred ratio is 1:0.02 to 1:0.09. The preparation may be administered to the ocular surface by various methods. For example, the preparation may be applied topically to the ocular surface as in drops, ointments, or gels. The preparation may also be applied by means of an eye cup such that the eye is bathed. In addition, the preparation may be used with a continuous or nearly-continuous infusion device for ocular surface irrigation and/or wetting and/or drug delivery. The preparations have broad ophthalmic utility by themselves, as well as in combination with pharmaceutically and/or physically active components when it is desirable to utilize topical therapy in the eye while minimizing ocular surface toxicity. For a fuller understanding of the invention, reference should be made to the following detailed description and accompanying drawings, which are by way of example of the invention. In the drawings, Figure 1 shows comparative features of the invention in the form of a graph of the percentage mucus-containing goblet cells remaining after bathing for 12 hours, relative to certain controls, as a function of potassium concentration; Figure 2 shows comparative features of the invention in the form of a graph of the percentage mucus-containing goblet cells remaining after bathing for 12 hours, relative to the controls, as a function of bicarbonate concentration; Figure 3 shows comparative features of the invention in the form of a graph of the percentage mucus-containing goblet cells remaining after bathing for 12 hours, relative to the controls, as a function of sodium concentration; and Figure 4 shows comparative features of the invention in the form of a graph of the percentage mucus-containing goblet cells remaining after bathing for 12 hours, relative to the controls, as a function of chloride concentration. The invention further provides a method for preparing a synthetic non-toxic ophthalmic preparation for topical application and/or infusing to the human or animal ocular surface, wherein said preparation includes potassium, chloride, bicarbonate, and sodium, and the method comprises the steps of (a) providing 22.0 to 43.0 millimoles of potassium per liter, (b) providing 29.0 to 50.0 millimoles of bicarbonate per liter, (c) providing 130.0 to 140.0 millimoles of sodium per liter, and (d) providing 118.0 to 136.5 millimoles of chloride per liter. In accordance with the invention, a non-toxic ophthalmic preparation combines multiple components to form a preparation which can be topically applied to the ocular surface by whichever of various methods is desired. The non-toxic ophthalmic preparation of the invention is prepared to attain between 22.0 to 43.0 mMoles/liter of potassium, 118.0 to 136.5 mMoles/liter of chloride, 29.0 to 50.0 mMoles/liter of bicarbonate, 130.0 to 140.0 mMoles/liter of sodium, 0.5 to 2.0 mMoles/liter of calcium, 0.3 to 1.1 mMoles/liter of magnesium, and 0.8 to 2.2 mMoles/liter of phosphate. Although it is generally desirable for the preparations to be essentially isotonic, the final osmolality or tonicity of the solution may vary. Indeed, since hypotonic and hypertonic eyedrops are brought rapidly to isotonicity by the exit or entrance, respectively, of water from the tear film, as first demonstrated by Maurice (Exp Eye Res 11, 30, 1971), and as later confirmed directly by Holly and Lamberts (Invest Ophthalmol Via Sci 20, 236, 1981), the preferred embodiment may be diluted to hypotonic concentrations when this is therapeutically desirable. One instance occurs in the treatment of elevated tear film osmolarity, such as disclosed in U.S. Patent No. 4,371,522. The preparation may also be concentrated to hypertonic concentrations when therapeutically desirable, such as in topical formulations for the treatment of recurrent corneal erosions. The preferred resulting solution has a pH value between 7.0 and 8.0 (as measured with a Fisher pH Accumet Model 600). Nevertheless, this pH range need not be rigidly adhered to, and it may be desirable to alter pH outside of this range, for instance to improve ophthalmic drug penetration through the ocular surface. Those skilled in the art may, in view of the teachings herein, employ other pH ranges. The effect of various ophthalmic preparations on mucus-containing goblet cell density has been examined and can be further understood from the following experiments and results. It is recognized that results of tests using rabbits has close correlation with humans and therefore the results carry over to humans. In vivo tests of various ophthalmic preparations, some according to the invention, and some according to prior art, were carried out in anesthetized rabbits. Their lids were elevated with sutures attached to elevated posts to form a conjunctival well. In separate experiments the well was filled with test solutions such that the entire ocular surface was submerged. One eye of each rabbit was bathed with a test solution, the other eye was not treated and served as a control. Baths were exchanged every hour for a twelve-hour period with fresh solution that had been bubbled with air. At the completion of the twelve-hour period, four conjunctival biopsies were taken from the eye that had been bathed, as well as from the contralateral control eye that had been closed for the twelve-hour period. Biopsies were taken adjacent to the limbus with a 5 mm trephine blade in the superotemporal, superonasal, inferotemporal and inferonasal quadrants. In some experiments only two biopsies were taken from each eye. Tissue was mounted flat and stained for mucus-containing goblet cells with PAS. Goblet cells were counted in eight 0.3 square mm contiguous squares beginning immediately at the limbal margin of the biopsy and counting peripherally in the direction of the conjunctival fornices. Goblet cell densities were averaged for each eye and compared to the average density in the contralateral control eye. Tests were run with twelve solutions, five of which are known solutions and seven of which were prepared according to the invention. The known solutions which were tested and which are further described below included Unisol (TM) , Balanced Salt Solution (R) , Lactated Ringer's Solution, Graeme S. Wilson's Solution, and Medium 199. The solutions prepared according to the invention are Example Solutions A-G, the constituents and properties of which are further described in Table II. The known solutions that were tested are described as follows: 1) Unisol (TM) (may be obtained commercially from CooperVision). A sterile preservative-free buffered isotonic aqueous solution containing sodium chloride, boric acid and soduim borate; Osmolarity; 302 m0sm/L, (as measured with a Clifton Nanolitre Osmometer); pH:7.0. (as measured with a Fisher pH Accumet Model 600). 2) Balanced Salt Solution (R) (may be obtained commercially from Alcon Laboratories). 3) Lactated Ringer's Solution (may be obtained commercially from Abbott Laboratories). 4) Graeme S. Wilson's Solution (as described at the International Tear Film Symposium - 1984, Lubbock, Texas). Potassium Chloride: 18.7 mM/L Sodium Chloride: 116.4 mM/L Sodium Bicarbonate: 25.9 mM/L Calcium Chloride: 0.4 mM/L Magnesium Chloride: 0.6 mM/L Sodium Phosphate: 0.7 mM/L 5) Medium 199 with Hank's Balanced Salt Solution and L-Glutamine modified with 1.4 g Sodium Bicarbonate per liter (may be obtained commercially from M. A. Bioproducts). The results of tests carried out using the known solutions are indicated in Table I and the results of tests carried out using solutions prepared according to the invention are indicated in Table III. As indicated in Tables I and III, the solutions prepared according to the invention attained a higher percentage of mucus-containing goblet cells remaining as compared to the known solutions. The high percentage of goblet cells remaining indicates the non-toxicity and normal maintenance of the ocular surface achieved by Solutions A-G. The percentage mucus-containing goblet cells remaining is high not only for solutions A-F, which contain potassium, chloride, calcium, magnesium, sodium phosphate and bicarbonate, but is also high for solution G, which contains only potassium, chloride, sodium and bicarbonate. The results from tests carried out using the various known and Example Solutions are also indicated in Figures 1-4. The figures demonstrate the percent of mucus-containing goblet cells remaining as a function of the concentration of particular constituents in the various solutions. As can be seen in the Figures, varying the concentrations of the constituents significantly affects the percentage of mucus-containing goblet cells remaining. The described ophthalmic preparations can be used as an artificial tear solution, as an ocular wetting solution, as a drug vehicle, as a solution for increasing eye comfort, as a contact lens wetting solution, as well as a contact lens soaking solution. The preparations can be administered to the eye according to conventional practices, including topically as a drop, in an ointment, and as a gel. It can also be applied to the eye via an eye cup, or infused into the tear film on a continuous or nearly continuous basis with a pump and catheter type of system. In the foregoing examples, the solvent was water which is generally the preferred solvent, but other solvents can be used. Other eye preparations such as ointments or gels may require other solvents, for example petroleum derivatives such as mineral oil. It will thus be seen from the preceding description that the objects of the invention are efficiently attained.

Claims: Claims for the following Contracting States: BE, CH, DE, FR, GB, IT, LI, LU, NL, SE A synthetic non-toxic ophthalmic preparation containing potassium, chloride, bicarbonate, and sodium, said preparation comprising (a) between 22.0 to 43.0, preferably between 23.0 to 42.0, millimoles of potassium per liter, (b) between 29.0 to 50.0, preferably between 31.0 and 48.0, millimoles of bicarbonate per liter, (c) between 130.0 to 140.0, preferably between 131.0 to 139.0, millimoles of sodium per liter, and (d) between 118.0 to 136.5, preferably between 124.0 to 136.0, millimoles of chloride per liter. A synthetic non-toxic ophthalmic preparation containing potassium, chloride, bicarbonate, and sodium, said preparation comprising a) a ratio of potassium to bicarbonate between 1:0.6 and 1:2.3, e.g. between 1:0.7 to 1:2.1, millimoles per liter, b) a ratio of potassium to sodium between 1:3.0 and 1:6.4, e.g. between 1:3.1 to 1:6.0, millimoles per liter, and c) a ratio of potassium to chloride between 1:2.7 and 1:6.2, e.g. between 1:2.9 to 1:5.9 millimoles per liter. The preparation according to claim 1 or claim 2, which is substantially free of at least one of the naturally-occurring tear fluid constituents selected from histamine, immunoglobulins, complement, desquamated epithelial cells, and bacteria or micro-organisms. The preparation according to claim 1, 2 or 3, which is isotonic. The preparation according to claim 1, 2, 3 or 4, which has a pH value between 7.0 and 8.0. The preparation according to any of claims 1 to 5, further comprising at least one of the following: calcium, magnesium, and phosphate. The preparation according to claim 6, wherein (a) said calcium is present in a concentration of between 0.5 to 2.0, e.g. between 0.6 to 0.8, millimoles per liter, (b) said magnesium is present in a concentration of between 0.3 to 1.1, e.g. between 0.5 to 0.6, millimoles per liter, (c) said phosphate is present in a concentration of between 0.8 to 2.2, e.g. between 1.0 to 2.0, millimoles per liter. The preparation according to claim 6, wherein the potassium content is 23.0 to 42.0 millimoles/liter, the bicarbonate content is 31.0 to 48.0 millimoles/liter, the sodium content is 131.0 to 139.0 millimoles/liter and the chloride content is 124.0 to 136.0 millimoles/liter, said calcium is present in a concentration of between 0.5 to 2.0, e.g. 0.6 to 0.8, millimoles per liter, said magnesium is present in a concentration of between 0.3 to 1.1, e.g. 0.5 to 0.6, millimoles per liter, and said phosphate is present in a concentration of between 0.8 to 2.2, e.g. 1.0 to 2.0, millimoles per liter. A synthetic non-toxic ophthalmic preparation containing potassium, chloride, bicarbonate, sodium, calcium, magnesium, and phosphate, the preparation comprising a) a ratio of potassium to bicarbonate between 1:0.6 and 1:2.3, e.g. between 1:0.7 and 1:2.1, millimoles per liter, b) a ratio of potassium to sodium between 1:3.0 and 1:6.4, e.g. between 1:3.1 and 1:6.0, millimoles per liter, c) a ratio of potassium to chloride between 1:2.7 and 1:6.2, e.g. between 1:3.0 and 1:5.9, millimoles per liter, d) a ratio of potassium to calcium between 1:0.01 and 1:0.09 millimoles per liter, e) a ratio of potassium to magnesium between 1:0.005 and 1:0.05 millimoles per liter, and f) a ratio of potassium to phosphate between 1:0.015 and 1:0.1 millimoles per liter. The preparation according to claim 9, which comprises a ratio of potassium to calcium between 1:0.1 and 1:0.04 millimoles per liter, a ratio of potassium to magnesium between 1:0.01 and 1:0.03 millimoles per liter, and a ratio of potassium to phosphate between 1:0.02 and 1:0.09 millimoles per liter. A method for preparing a synthetic non-toxic ophthalmic preparation for topical application and/or infusing to the human or animal ocular surface, wherein said preparation includes potassium, chloride, bicarbonate, and sodium, and the method comprises the steps of (a) providing 22.0 to 43.0 millimoles of potassium per liter, (b) providing 29.0 to 50.0 millimoles of bicarbonate per liter, (c) providing 130.0 to 140.0 millimoles of sodium per liter, and (d) providing 118.0 to 136.5 millimoles of chloride per liter. The method according to claim 11, comprising a) providing a ratio of potassium to bicarbonate of 1:0.6 to 1:2.3, e.g. 1:0.7 to 1:2.1, millimoles per liter, b) providing a ratio of potassium to sodium of 1:3.0 to 1:6.4, e.g. 1:3.1 to 1:6.0, millimoles per liter, and c) providing a ratio of potassium to chloride of 1:2.7 to 1:6.2, e.g. 1:2.9 to 1:5.9 millimoles per liter. Claims for the following Contracting State: AT A method for preparing a sythetic non-toxic ophthalmic preparation for topical application and/or infusing to the human or animal ocular surface, wherein said preparation includes potassium, chloride, bicarbonate, and sodium, and the method comprises the steps of (a) providing 22.0 to 43.0 millimoles of potassium per liter, (b) providing 29.0 to 50.0 millimoles of bicarbonate per liter, (c) providing 130.0 to 140.0 millimoles of sodium per liter, and (d) providing 118.0 to 136.5 millimoles of chloride per liter. The method according to claim 1, comprising (a) providing a ratio of potassium to bicarbonate of 1:0.6 to 1:2.3, eg. 1:0.7 to 1:2.1 millimoles per liter, (b) providing a ratio of potassium to sodium of 1:3.0 to 1:6.4, eg. 1:3.1 to 1:6.0 millimoles per liter, and (c) providing a ratio of potassium to chloride of 1:2.7 to 1:6.2, eg. 1:2.9 to 1:5.9 millimoles per liter. The method according to claim 1 or claim 2 wherein the resulting preparation is substantially free of at least one of the naturally-occurring tear fluid constituents selected from histamine, immunoglobulins, complement, desquamated epithelial cells, and bacteria or micro-organisms. The method according to claim 1, 2 or 3 wherein the preparation is isotonic. The method according to claim 1, 2, 3 or 4 wherein the preparation has a pH value between 7.0 and 8.0. The method according to any of claims 1 to 5 wherein there is further provided at least one of the following: calcium, magnesium and phosphate. The method according to claim 6, wherein (a) said calcium is provided in a concentration of between 0.5 to 2.0, eg. between 0.6 to 0.8 millimoles per liter, (b) said magnesium is provided in a concentration of between 0.3 to 1.1, eg. between 0.5 to 0.6 millimoles per liter, (c) said phosphate is provided in a concentration of between 0.8 to 2.2, eg. between 1.0 to 2.0 millimoles per liter. The method according to claim 6, wherein the potassium content is 23.0 to 42.0 millimoles/liter, the bicarbonate content is 31.0 to 48.0 millimoles/liter, the sodium content is 131.0 to 139.0 millimoles/liter and the chloride content is 124.0 to 136.0 millimoles/liter, said calcium is present in a concentration of between 0.5 to 2.0, eg. 0.6 to 0.8 millimoles per liter, said magnesium is present in a concentration of between 0.3 to 1.1, eg. 0.5 to 0.6 millimoles per liter, and said phosphate is present in a concentration of between 0.8 to 2.2, eg. 1.0 to 2.0 millimoles per liter. A method for preparing a synthetic non-toxic ophthalmic preparation containing potassium, chloride, bicarbonate, sodium, calcium, magnesium, and phosphate, the method comprising providing (a) a ratio of potassium to bicarbonate between 1:0.6 and 1:2.3, eg. between 1:0.7 and 1:2.1 millimoles per liter, (b) a ratio of potassium to sodium between 1:3.0 and 1:6.4, eg. between 1:3.1 and 1:6.0 millimoles per liter, (c) a ratio of potassium to chloride between 1:2.7 and 1:6.2, eg. between 1:3.0 and 1:5.9 millimoles per liter, (d) a ratio of potassium to calcium between 1:0.01 and 1:0.09 millimoles per liter, (e) a ratio of potassium to magnesium between 1:0.005 and 1:0.05 millimoles per liter, and (f) a ratio of potassium to phosphate between 1:0.015 and 1:0.1 millimoles per liter. The method according to claim 9, which comprises providing a ratio of potassium to calcium between 1:0.1 and 1:0.04 millimoles per liter, a ratio of potassium to magnesium between 1:0.01 and 1:0.03 millimoles per liter, and a ratio of potassium to phosphate between 1:0.02 and 1:0.09 millimoles per liter.

Inventors: GILBARD, JEFFREY P.
Applicants: EYE RESEARCH INSTITUTE OF RETINA FOUNDATION
Application year: 1986
Main classification: A61K33
IPC codes: A61K33, A61P27, A61K9