LECTIN-ANTIBODY SANDWICH ASSAY FOR DESIALYLATED GLYCOPROTEINS

Abstract: A method for determining the presence of soluble desialylated glycoproteins in biological fluids. The method comprises contacting a sample of the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a complex. The resulting complex is separately recovered from the biological fluid. The recovered complex is contacted under appropriate conditions with at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex. The presence of antibodies so bound is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid determined.

Description: - LECTIN - ANTIBODY SANDWICH ASSAY FOR DESIALYLATED ClrTOPRPTEINS Background of the Invention The invention described herein was made with Government support under grant numbers HD15454-03 and RR00645-13 from the National Institute of Child and Health Development, United States Department of Health and Human Services. The Government has certain rights in the invention. Within this application several publications are referenced by arabic numerals within parentheses. Full citations for these references may be found at the end of the specification immediately preceding the claims. The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains. Human chorionic gonadotropin (hCG) purified from urine of pregnant women has been shown to have approximately 30% carbohydrate content, consisting of two N- asparagine linked carbohydrate side chains on each of its and subunits (1) and four O-serine linked side chains attached to the COOH-terminal peptide region of its subunit (2,3). In 1981 Nishimura et al. (4) observed that hCG purified from the urine of a patient with choriocarcino a had a reduced carbohydrate content and that this difference was due to a low sialic acid content. Later studies of the hCG from the same patient (5) revealed unusual N-asparagine linked oligosaccharide side chains of which 97% were free of sialic acid. Studies by A r et al. (6,7) have indicated that the porportion of total hCG which is desialylated in the urine of women with choriocarcinoma is somewhat variable but is consistently higher than that from pregnancy urine. The latter studies also indicated that in addition to desialylated hCG, a small molecule with properties similar to those of the free desialylated hCG COOH-terminal peptide (as 3CTP) was present in the urine of women with choriocarcinoma but not in pregnancy urine. Those findings suggested that excretion of desialylated forms of hCG might be characteristic of patients with choriocarcinoma and measurement of these forms might be valuable in diagnosis and management of the disease. Highly specific antisera to asBCTP have been developed (8) and applied to the detection of desialylated forms of hCG in clinical situations (6,7). However, only moderate sensitivity can be obtained in RIA systems using these antisera. In addition, analyses of urine specimens using these antisera have previously relied upon sample preparation by gel filtration chromatography (6,7). Clinical studies of the production of desialylated forms of hCG by patients with trophoblastic neoplasia would be facilitated by the availability of simple yet highly sensitive and specific methods for the detection of ashCG in urine. An alternate approach to the detection of glycoproteins is to utilize lectins. These carbohydrate binding proteins, have proved to be useful reagents for probing structural features of cell surface glycoproteins and for isolating glycoproteins. In the present study we have exploited the carbohydrate binding properties of two lectins, peanut lectin, fAraehis hypogea agglutinin, PNA) , and castor bean lectin, .Ricinus commupfts agglutinin, RCA) , to develop highly specific methods for the detection of aεhCG in urine. PNA and RCA specifically bind the desialylated 0-serine (9,10) and N-aεparagine (9) linked oligosaccharides of hCG respectively. Solid phase lectin is utilized to extract ashCG from urine, and lectin bound ashCG is then measured utilizing a purified and radiolabeled monoclonal antibody or rabbit antiserum. The form of desialylated hCG detected is dependent not only on the carbohydrate specificity of the lectin but the peptide specificity of the monoclonal antibody or antiserum employed. This unique type of assay has been described as a Lectin-Immunoradiometric Assay (LIRHA) and may be extended to the measurement of a variety of soluble glycoproteins by using combinations of lectins and antibodies with different specifications. Lectins are proteins or glycoproteins of non-immune origin long known to be useful for agglutinating erythrocytes and other types of cells and useful for studying cell surface properties. However, as macro olecules it might be unexpected that the binding of a lectin to a glycoprotein, particularly a relatively small glycoprotein such as hCG, would prevent subsequent or contemporaneous binding of an antibody directed to an antigenic determinant on the glycoprotein. Unexpectedly, it has been found that the combined use of a lectin which selectively binds to a specific sugar moiety and of an antibody provides a highly sensitive and specific method for qualitatively detecting or quantitatively determining a desialylated glycoprotein. Specifically the method provided permits exploitation of the dual nature of glycoproteins. The lectin component binds specifically to the carbohydrate moiety while the antibody component binds specifically to the peptide moiety. As a result, one may distinguish glycoproteins on the basis of a carbohydrate moiety which may be critical if the peptide regions are identical. An example of the importance of the invention is the ability to distinguish desialylated hCG from εialylated hCG and, thereby, diagnose gestational trophoblastic disease. Summarγ of the Invention A method for determining the presence of a soluble desialylated glycoprotein such as human chorionic gonadotropin in a biological fluid such as urine or blood is provided. This method comprises contacting a sample of the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a complex. Examples of such lectins include peanut lectin and castor bean lectin. The resulting complex is separately recovered from the biological fluid. The recovered complex is contacted under appropriate conditions with at least one detectable antibody such as radiolabeled or fluorescently labeled monoclonal antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex. Ωie presence of antibody so bound is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid determined. A method for quantitatively determining a soluble desialylated glycoprotein in a biological fluid is also provided. This method comprises contacting a sample of the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a complex. The resulting complex is separately recovered from the biological fluid. The complex so recovered is contacted under appropriate conditions with a predetermined amount of at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex. The amount of antibody so bound is determined and, thereby, the amount of desialylated glycoprotein in the biological fluid determined. Thus, this invention provides both qualtitative methods for detecting and quantitative methods for determining the presence in biological fluids of soluble glycoproteins. As will be clear to those skilled in the art to which this invention relates, these methods may be readily modified in several respects. Thus, the lectin rather than the antibody may be detectably labeled; the antibody rather than the lectin may be employed to initially contact the sample or the sample may be simultaneously contacted with the antibody and the lectin. -I- Brief Description of the Figures Figure 1: R141 RIA of ashCG with extraction method (• •) and direct method ( ▲ Jk ), and hCG with extraction method (o o) and direct method (Δ Δ)» The extraction method uses a solid phase coupled monoclonal antibody to hCG βsubunit for extraction of hCG or ashCG from buffer or urine. Figure on lower right corner: R141 RIA (extraction method) of ashCG from buffer (o o) and normal male urine (o o) . Figure 2: Characteristics of four lectin immunoradiometric assays (LIRMA) for measurement of ashCG in buffer or urine. Comparisons of dose responses were made for the following hormones or fragments: AshCG (o o) , ashCGB (o o) , asδ-CTP (d O ), hCG ( ^), hCG ( Δ ___ ) and hLH (β g ) # τhe LIRMAs illustrated are as follows : A : PNA- 125 I-R525, B: PNA- 125 I-B105, C: PNA- 125 I-B107, D: RCA- 125 I-R525. Inserts on upper right corners of panels A and D illustrate measurement of ^ ashCG in buffer ( O o ) and normal male urine ( O O ) using PNA- 12 Λ 5 -R525 (A) and RCA- 125 -I R525 (D) . Figure 3 : Sephadex G-100 gel filtration of a urine concentrate of a patient with choriocarcinoma (panel A) and of a normal pregnant woman (panel B) . 10 ml of the urine concentrate was applied to a col umn (2.5 x 196 cm) of Sephadex G-100 , and eluted with 0.05 M Tris-HCl buffer containing 0.1 H NaCl (pH 7.4) . Fractions of 6 ml were collected and al iquots were assayed in various assay systems . Total hCG was determined by R529 RIA (•) , and ashCG by R141 RIA (direct method) (o) and PNA- 125 I-R525 ( ▲ ) LIRMA. 100 ,000 cpro of 125 I-hCG in 10 ml of the same buf fer was eluted in separate run as a marker . The insert in panel B illustrates the amounts of asi alo hCG immunoreactivity detected using the three methods on an expanded scale. Detailed Description of the Invention A method is provided for determining the presence of a soluble desialylated glycoprotein in a biological fluid which comprises contacting a sample of the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a complex. The resulting complex is separately recovered from the biological fluid. The so recovered complex is contacted under appropriate conditions with at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex. The presence of antibody so bound is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid is determined. The soluble desialylated glycoprotein may be hCG, thyroglobulin (11), carcinoembryonic antigen or CA19-9 (12) , or any other desialylated glycoprotein or subunit thereof, e.g. desialylated hCG. The biological fluid may be urine, blood, semen, saliva, pus or any other biological fluid, a sample of which one wishes to examine. Typcially, biological fluid will be obtained from a subject, e.g. a human patient. Thus, for example, desialylated hCG may be detected in human urine. A sample of the biological fluid is obtained and contacted with a suitable amount of an appropriate lectin to produce a complex.- The contact typically involves simply adding the lectin to the sample under ambient conditions. The amount and type of lectin added may vary widely depending upon factors well known in the art such as the concentration of soluble glycoprotein normally present in the sample and the nature of the glycoprotein. Typcially, the amount of lectin added will be in molar excess of the amount of glycoprotein present in the sample. Appropriate lectins are lectins capable of selectively binding to the desialylated glycoprotein. Numerous lectins are known which selectively bind to specific sugar moieties and which may therefor be employed in this invention. For example, peanut lectin may be derived from Arachis hypoσea. Such lectin selectively binds to the carbohydrate structure GalS 1—^3GalNAc. Another example is castor bean lectin derived from Ricinus commuηis which selectively binds to the carbohydrate structure Gal31— 4GlcNAc. The complex which results from contacting the sample of biological fluid with the lectin is then separated from the biological fluid, e.g. by centrifugation followed by decantation of the supernatant. However, other methods of recovery may be employed such as filtration. The separately recovered complex is contacted with at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex. Appropriate conditions for effecting such contact are well known to those skilled in the art, e.g. the complex may be rediεsolved in a suitable buffer and the antibody, dissolved in the same or a compatable buffer added at a temperature, e.g. 37°c, at which activity of the antibody is retained. The antibody used in this method may be either a polyclonal or a monoclonal antibody which is detectable e.g. because an identifiable label such as a radiolabel or a fluorescent label has been attached to it. Alternatively, the antibody may be detected by use of a second antibody directed it, the second antibody being labeled or having an enzyme substrate bound to it. For example, if the glycoprotein to be detected is desialylated hCG the antibody may be a serum or monoclonal antibody directed to a determinant on the subunit such as an antibody directed to the carboxy terminal region of the subunit. In the experiments which follow asialo hCG, a type of desialylated hCG, has been detected. It is to be understood that desialylated is intended to encompass both glycoproteins which do not naturally include sialo groups and these which naturally include sialo groups, but from which they have been removed. The latter situation is believed to be a general indicator of disease and has been so implicated in choriocarcinoma and hydatiform mole. The presence of antibody bound to the lectin- glycoprotein complex may be readily detected using well known techniques. Thus, if the antibody is fluorescently labeled with a moiety such as a fluorescent dye covalently bound to the antibody the fluorescent emission of the dye upon excitation with appropriate electromagnetic radiation such as ultraviolet radiation may be measured or detected using a conventional fluorimeter. In a similar manner a radioactive isotope such as I 125 bound to the antibody may be detected using a coventional scintillation spectrometer. By comparing the results obtained using such methods, e.g. the amount of radioactivity, with those obtained using a control sample one may determine that the desialylated glycoprotein is present in the biological fluid. A method for quantitatively determining the amount of a soluble desialylated glycoprotein in a sample of a biological fluid comprises contacting the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a complex. The resulting complex is separately recovered from the biological fluid. The complex so recovered is contacted under appropriate conditions with a predetermined amount of at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex. The amount of antibody so bound is determined and, thereby, the amount of desialylated glycoprotein in the biological fluid determined. In one embodiment, the desialylated glycoprotein to be quantitatively determined is hCG. One application of the methodology for quantitatively determining soluble desialylated glycoprotein involves the diagnosis of a disease associated elevated levels of desialylated hCG such as choriocarcinoma or hydatiform mole. By quantitatively determining the amount of desialylated hCG in a biological fluid from a patient and comparing this amount with the amount determined for a normal patient, diseases such as choriocarcinoma or hydatiform mole may be diagnosed. Alternatively, a method for determining the presence of soluble desialylated glycoprotein in a biological fluid comprises contacting a sample of the biological fluid with at least one antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of binding to the glycoprotein to produce a complex. The resulting complex is separately recovered from the biological fluid. The so recovered complex is contacted with a suitable amount of an appropriate detectable lectin. The presence of the lectin so bound is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid is determined. The detectable lectin is radiolabeled or fluorescently labeled. In the same manner as described previously for the method in which the sample is contacted first with a lectin and then with a detectable antibody, the preceding method may be rendered quantitative as may the various related methods which follow. The invention also provides a method for determining the presence of a soluble desialylated glycoprotein in a biological fluid which comprises contacting a sample of the biological fluid with a suitable amount of an appropriate detectable lectin capable of selectively binding to the desialylated glycoprotein to produce a complex. The complex, under appropriate conditions, is contacted with at least one antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein in the complex. The resulting complex is separately recovered from the biological fluid. The presence of lectin bound to the recovered complex is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid is determined. The preceding method may be carried out in a different sequence. Thus a method for determining the presence of soluble desialylated glycoprotein in a biological fluid comprises contacting a sample of the biological fluid with at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of binding to the glycoprotein to produce a complex. The complex is contacted with a suitable amount of an appropriate lectin capable of selectively binding to glycoprotein present in the complex. The resulting complex is separately recovered from the biological fluid. The presence of antibody bound to the recovered complex is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid is determined. In another embodiment, the invention provdies a method for determining the presence of a soluble desialylated glycoprotein in a biological fluid which comprises subtantially concurrently contacting a sample of the biological fluid under appropriate conditions with both a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a recoverable complex and at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein capable of selectively binding to the glycoprotein as well as to the recoverable complex. The resulting complex is separately recovered from the biological fluid. The presence of antibody bound to the recovered complex is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid is determined. The preceding method may be varied by substituting a detectable lectin for the detectable antibody. Thus, a method for determining the presence of soluble desialylated glycoprotein in a biological fluid comprises contacting a sample of the biological fluid under appropriate conditions with both an antibody directed to an antigenic determinant on the desialylated glycoprotein to produce a recoverable complex and a suitable amount of an appropriate detectable lectin capable of selectively binding to the desialylated glycoprotein as well as to the recoverable complex. The resulting complex is separately recovered from the biological fluid. The presence of lectin bound to the recovered complex is detected and, thereby, the presence of desialylated glycoprotein in the biological fluid is determined. Experi ental Detail s Reagents : HCG, (CR121; biopotency, 13,450 Iϋ/mg Second International Standard) , hCG 3 (CR123) ,3-CTP (hCG 123- 145) and their asialo forms (ashCG 3,asS-CTP, ashCGS 123-145) were prepared as described earlier (13-15) . HLH (hLH-I-1 AFP-4345B; biopotency, 6000 IU/mg WHO International Standard of urinary FSH/LH70) was provided by the National Pituitary Agency of NIAMDD. Antisera to 3 -CTP (R525 and R529) and as B-CTP (R141) were characterized previously (8,16). The characteristics of the monoclonal antibodies against hCG 3 (B101, B105 and B107) have been described elsewhere (17) . Monoclonal antibody B101 has an equilibrium association constant (Ka) of 7 x 108 M-l for hCG, and cross-reactivities of 9 and 2% for hCGS and hLH, respectively. The Ka of B105 for hCG is 1.5 x 1011 - 1 and it cross-reacts 100% with both hCG 3 and hLH. The Ka of B107 for hCG is 4 x 10 10 M- 1 and it has only 0.1% cross-reactivity with hCG and less than 0.5% cross-reactivity with hLH. Monoclonal antibody, B101, was conjugated to CNBr activated Sepharose 4B (Pharmacia Fine Chemicals, Piscataway, NJ) according to the manufacturer's instructions. Iodinated ashCG, hCG and antibodies were prepared using Na 12 ^-I (Amersham Corp., Arlington Heights, IL) with Iodogen (Pierce Chemcial Co., Rockford, IL) as an oxidizing agent as described by Fraker and Speck (18). Peanut lectin, Arachis hypogea agglutinin, (PNA) and castor bean lectin, Ricinus communis agglutinin, (RCA) were covalently linked to Agarose were obtained from E-Y Laboratories Inc. (San Hateo, Ca.) . Clinical specimens : First morning voided or 24 hours collected urines were obtained from patients with trophoblastic tumors at New England Trophoblastic Disease Center of Brigham and Women's Hospital, Boston, MA and at the Medical College of Wisconsin, Milwaukee, Wl. First morning voided or random urines were also collected from normal pregnant women at the Columbia Presbyterian Medical Center, New York, NY. Filtration of the urine on sephadex G-100: One hundred ml of urine from a woman with choriocarcinoma and a normal pregnant woman were lyophilized and rediεsolved in 10 ml or 0.05 M Tris-HCl buffer containing 0.1 M NaCl (pH 7.4). The samples were chromatographed on a column of Sephadex G-100 (2.5 x 196 cm) previously equilibrated with the same buffer. Radioim unoassays: RIAs of urine specimens processed by gel filtration were performed using antisera R529 (14) and R141 (8) to determine total hCG, ΘCTP and ashCG CTP immunoreactivitieε, respectively, as described by A r et al. (6). An extration step was added to the RIA using R141 as antiserum (R141 RIA) in order to improve its sensitivity and to apply it to the measurement of ashCG in urine specimens without gel filtration. Four ml of standards containing 0.05-1.0 pmoles ashCG/ml or urine specimens (previously adjusted to pH 7.4 with NaOH and centrifuged at 3,000 xg for 15 min) , were pipetted into 12 x 75 mm polystyrene tubes. Two hundred microliterε of 5% εuεpenεion of B101 conjugated Sepharoεe 4B (BlOl-Sepharoεe 4B) in assay buffer (buffer A: 0.1% bovine gamma-globulin, pH 7.4) were pipetted into each tube. The tubeε were capped, placed horizontally on a Labquake Shaker (Lab Industries, Berkeley, Ca.) and incubated for 2 hours at room temperature with εhaking in order to extract hCG from the samples. The tubeε were centrifuged for 30 min at 3,000 xg. The εupernatants were removed by aspiration and the pellets were washed with 2 ml of 0.01 M PBS (pH 7.4). One ml of 10% formic acid was added and the tubes incubated for 30 min at 3,000 xg. One-half ml aliquots of the εupernatantε were removed and pipetted into separate 12 x 75 mm polystyrene tubes. The formic acid was evaporated in a Savant Speed Vac Concentrator (Savant Inεtrumentε Inc., Hickεville, NY) and the residue was dissolved in 100 microliters of buffer A. The ashCG concentrationε were then determined using the R141 RIA. Lectin-immunoradiometric assays (LIRMA) : The conditions for the LIRMA'ε were optimized as follows: The amounts of PNA-Agarose and RCA-Agarose and time of incubation at room temperature required to give maximum adsorption of ashCG from buffer A were determined. The amount of iodinated antibody added to the assay was chosen by determining the amount of tracer which gave the highest ratio of specific counts bound over non-specific binding (NSB: binding of tracer in absence of ashCG) . Time studies of radiolabeled antibody binding indicated that equilibrium was not attained until 96 hours of incubation at 4°C However, since an incubation period of 48 to 72 hours was sufficient to obtain high asεay εensitivity, it was the chosen time of incubation. Prior to asεay, urine εampleε were adjuεted to pH 7.4 with NaOH and centrifuged at 3,000 xg for 15 min. Duplicate or triplicate 4 ml aliquotε of urine (standards containing appropriate concentration of ashCG in buffer A or buffer A alone for determination of NSB) were pipetted into 12 x 75mm polystyrene tubes. Two-hundred icroliters of 10% suspension of PNA- Agaroεe or RCA-Agaroεe in buffer A were pipetted into each tube. The tubeε were capped, placed horizontally on a Labquake Shaker and incubated for 2 hours at room temperature in order to extract aεhCG from the εampleε. The tubes were centrifuged for 30 min at 3,000 xg. The supernatantε were removed by aεpiration and the pellets were washed with 2 ml of wash buffer (buffer B: buffer A containing 1% Tween 20). One hundred microliters buffer A containing approximately 50,000 CPM tracer ( 125 I-R525, 125 I-B105 or 125 I-B107) were added to each tube. The samples were incubated for 48-72 hours at 4°C with shaking on a Bellco Shaker. The tubes were then washed two times with 2 ml of buffer B to reduce NSB. The radioactivity remaining after washing was determined in a Packard Auto-Gamma Scintillation Spectrometer. Data reduction for the generation of εtandard curveε and aεhCG concentrationε of urine specimens was accomplished using a four parameter logistic fit (19). Immunoradimetric asεay fIRMA. _ The IRMA of hCG waε conducted uεing BlOl-Sepharoεe 4B to extract hCG and radiolabeled purified antiserum to 3 -CTP (R525) to measure urinary hCG as described by Armstrong et al. (20). Results: R 41 RIA: Typical standard curves generated for the RIA with and without an extraction step are shown in Figure 1. The ashCG dosages which resulted in binding equivalent to 90% (ED 90 ) and 10% (ED 10 ) of Bo (binding in the abεence of aεhCG) were uεed as the limits of the usable range for the εtandard curves. The ED g0 and ED^Q ^ or ^ e direct method, which does not employ an extraction εtep, correεponded to 0.8 and 20 pmoleε aεhCG/ml reεpectively. In contraεt, the ED., and ED 1Q of the RIA utilizing an extraction εtep were 0.05 and 1.0 pmoleε aεhCG/ml, reεpectively. Thuε, the uεe of an extraction step resulted in approximately a 16 fold improvement in asεay senεitivity. The cross- reactivitieε of hCG in the aεεays with and without extraction step were: 0.69 and 0.15% at ED 5Q , 7.20 and 6.15% at ED 90 and 0.17 and 0.03% at ED, Q , respectively. Figure 1 alεo shows the dose responεe curve of ashCG extracted from a pool of normal male urine for which portions had been augmented with dosages identical to thoεe employed in the εtandard curve. The slope and ED 50 °^ tne °= ose responεe curve in the extraction method were -1.401 and 0.250 pmoles/ml for ashCG in urine, compared to -1.318 and 0.238 pmoles/ml for ashCG in buffer. These differences were not statistically significant. Intra- and interasεay variance for the extraction method have been eεtablished utilizing normal male urine to which 0.25 pmoles ashCG/ml were added. At thiε dosage of aεhCG, the coefficients of intra- and interasεay variance were 9.72% (n=10) and 17.81% (n=10) , respectively. Lectin- immunoradiometric assays : Typi cal standard curves for aεhCG binding in the LIRMA ' ε uεing PNA and RCA as extraction reagents and R525 , B105 or B107 as antibodies are shown in Fig. 2 A, B, C, D. The aεhCG doεageε which gave binding equivalent to 10% (ED 10 ) and 90% (ED 90 ) of the maximum binding (Bmax) are deεginated as the l imits of the usable range of the standard curves. ED ιo and ED 90 corresponded to 0 .01 and 2.5 pmoles ashCG/ml in PNA- 125 I-R525 system , 0 .0002 and 0 .05 pmoles ashCG/ml in 125 PNA- I-B105 εyεtem , 0.005 and 0.6 pmoles ashCG/ml in PNA- ^ I-R525 εystem . Thus , these assays could give 250 , 2500 , 120 and 100 fold usable ranges, respectively. The insert in Fig. 2A showε the doεe reponεe curveε of ashCG extracted wi th PNA from normal male urine whi ch had been augmented with dosageε identical to thoεe uεed in the standards. The dose response curve for ashCG in urine was esεentially identical to that for aεhCG in buffer A in the PNA- 125 I-R525 εyεtem. The εlope and ED 5 Q were 1.141 and 0.136 pmoleε/ml for ashCG in urine compared to 1 .051 and 0.122 pmoles/ml for aεhCG in buffer A in PNA- 125 l-R525 εyεtem . In contraεt, aε εhown in Fi g. 2D, the doεe reεponεe curve obtained using the RCA- 125I-R525 syεtem for meaεurement of aεhCG in urine and in buffer A do not coincide. Therefore, assays of clinical specimens were conducted uεing standards diluted in normal male urine in order to compensate for the lower recovery of hCG from urine by RCA. The dose responεe curveε for aεhCG3, aε3-CTP, hCG, hCG3 and hLH added to buffer A are also shown in Fig. 2. The cross-reactivities of these standards in the asεay εyεtems are summarized in Table 1. Whereas the PNA- 125 I-R525 εyεtem can detect aεhCG, aεhCG3 and free aε3- CTP, the PNA- 125 I-B105 εyεtem can only detect aεhCG and aεhCGS and the PNA- 125 I-B107 εyεtem detects only dimeric ashCG. Since RCA doeε not recognize asialo O-εerine linked carbohydrate side chains in the 3-CTP region, RCA- 125 I-R525 syεtem detectε only aεhCG and 3aεhCG . The cross-reactivitieε of hCG, hCG and hLH were low in all the assay systems. Intra- and inter asεay variance for these LIRMA's have been eεtabliεhed utilizing normal male urine containing the dosages of ashCG coinciding approximately with ED 50 - These are also shown in Table 1. Analvεeε of σel-f iltration urine concentrates: The gel filtration profiles of the concentrated urine from a choriocarcinoma patient and a normal pregnant woman are shown in Fig. 3. The elution profile of the choriocarcinoma urine concentrate had a major peak of hCG immunoreactivity as determined by an RIA using an antiserum to 3-CTP (R529) which coincided with the elution volume (Ve) of 1 5 I-hCG. A peak of ashCG which eluted under the peak of hCG waε detected using the R141 RIA and the PNA- 125 I-R525 and RCA- 125 I-R525 LIRMA's. The proportion of ashCG to toal hCG in thiε firεt peak was 17% using PNA- 125 I-R525, 16% using RCA- 125 I-R525 and 16% using the R141 RIA. A second peak of -CTP immunoreactivity composed of low molecular weight molecules waε alεo detected in the elution profile uεing the R529 RIA, along with a cor- Table 1 Range, Crossreactivity and Validation of Each Assay P A _125ι_ R 525 P A-125I_B105 PNA-^J.-BIOV RCA- 125 I-R525 R141-RIA (Extraction Method) Assay Range (pmoles ashCG/ml) 0.01 - 2.5 0.0002 - 0.5 0.005 - 0.6 0.002 - 0.2 0.05 - 1.0 Crossreactivity (%) I ashCG (reference) 100 100 100 100 100 ashOGβ 100 0.00 0.00 0.00 NT as -CTP 100 0.00 0.00 0.00 NT hOG 0.1 0.06 0.00 0.03 0.7 hOGβ 0.03 0.15 0.00 NT NT hl_3 0.00 0.15 0.00 KΓΓ NT Variance (%) Intra-assay 6.8 8.3 9.0 5.1 9.7 Inter-assay 18.6 20.1 22.3 16.3 17.8 NT = not tested reεponding peak of a ε 3-CTP immunoreactivi ty detected by the R141 and PNA- 125 ι-R525 LIRMA. However , the RCA- 125 I-R525 εyεtem did not detect the aεhCG to total hCG in the second peak was 110% in PNA- 125 I-R525 and 115% in R141 RIA. In contrast, the gel fil tration el ution prof ile for the normal pregnancy urine concentrate had only a single peak of 3-CTP immunoreactivi ty eluting in the vicini ty of 125 l-hOG aε determined by the R529 RIA. Alεo, no aεhCG immunoreactivity waε detected wi thin thiε region using the R141 RIA; whereas , very sl ight amounts were detected using the PNA- I-R525 LIRMA ( 0.18% of total hCG ) and the RCA- 125 l-R525 LIRMA ( 0.14% of total hCG) . Crossreactivity of hCG in the various asεays (Table 1 ) could account for these small amounts of apparent asialo hCG immunoreactivity. Assays of cl inical spcimens . The PNA- 125 I-R525 LIRMA, RCA- 125 I-R525 LIRMA and R141 RIA wi th an extraction step were util ized to measure ashCG in urine speci enε from patientε with geεtational trophoblastic tumors and women during normal pregnancy. The total concentrations of hCG in these specimens were determined util izing the B101-R525 IRMA (20) . The means of the percentages of asi alo hCG concentrations over toal hCG concentrations obtained using the three different methods are εhown in Table 2. The Newman-Keulε Mul tiple Range Test ( 21) was used to determine if the mean valueε for the three groups of subjectε were εignifi cantly different from each other . The mean percentageε of aεialo hCG over total hCG concentration for εpecimenε from choriocarcinoma patients obtained using all three assays were signifi cantly different from those for hydatidiform \ 0 Table 2 _ Mean Percentages of Asialo hCG Over Total hOG Concentrations + Standard Deviations in -Specimens frcm Wαnen with Gestational Trophoblastic Tumors and Normal Pregnant Wαnen M≥thod Pregnancy Hydatidiform Mole Choriocarcinoma R141 RIA (Extraction Method) 0.937 + 1.276 3.384 + 3.739* 11.765 + 8.76** (n = 11) (n = 11) (n = 6) PNA-R525 LIRMA 0.049 + 0.045 2.693 + 3.542* 13.726 + 7.350** (n = 6) (n = 10) (n = 6) RCA-R525 LIRMA 0.178 + 0.304 0.504 + 1.000* 2.044 + 0.709** (n = 10) (n = 9) (n = 5) * Not significantly different (p t«05) from normal pregnancy **Significantly different (p §.05) frαn normal pregnancy and hydatidiform mole en mole and normal pregnancy. However, the valueε for hydatidiform mole were not significantly different from normal pregnancy in any of the assays. Discussipn: Several reports have indicated that the proportion of asialo forms of hCG present in the urine of choriocarcinoma patients are in marked excess relative to the levels in the urine during normal pregnancy (4- 7) . Although measurements of ashCG might be useful in the detection of trophoblastic tumors, routine asεayε for ashCG for use in clinical studieε have not been developed previouεly. We have examined the use of new aεεay systems to measure ashCG in urine specimens and conducted a preliminary investigation of their clinical utility. These methods can be used to obtain highly specific and sensitive measurementε of aεhCG. They alεo offer greater ease of performance than the previously used methodology which required gel filtration of εpecimenε prior to RIA (6,7). The croεs- reactivitieε of hCG, hCG3 and hLH are very low in all of the newly developed εystems. The extreme selectivity of the LIRMA for asialo forms of hCG can be attributed to the carbohydrate specificity at the lectins employed. Peanut lectin, (PNA) , has high specificity for the terminal carbohydrate structure Gal31 — > 3GalNAc (9,10); and castor bean lectin (RCA) , is highly specific for the terminal structure of Gal31- J »4GlcNAc (9). When sialic acid is removed from hCG, its unique 3-CTP region has four εtructureε of O-εerine linked Gal31— »3GalNAc (2), and each of itε and εubunitε has two εtructureε of N- asparagine linked Gal31 — *4GlcNAc (1). Therefore, PNA and RCA recognize aεialo O-εerine and N-aεparagine linked carbohydrate εide chains, respectively. Aε εummarized in Table 3 , the LIRMAs have the abili ty to distinguish between various formε of aεialo hCG based on the specificitieε of the lectinε and antibodieε employed. The PNA- i *"'l-R525 εyεtem detects ashCG, aεhCG 3 and f ree aε3CTP, indicating simul taneous binding of the lectin and antibody to the CTP region. This finding was unexpected considering the potential for steric hindrance of binding two large molecules to thiε relatively small peptide. The PNA- 125 I-B105 εyεtem, which uεeε a monoclonal antibody to hCG3 , detects ashCG and ashCG3 * Since B107 recognizes only dimeric hCG , PNA- 1 25 I-B107 detects only ashCG . PNA recognizeε aεialo O-serine l inked carbohydrate moieties which are local ized in 3-CTP region (2 ,10) and RCA recognizes N-asparagine linked carbohydrate moieties which are in α and subuni ts (1) . Thiε difference in demonεtrated by the inabili ty of the RCA- 125 I-R525 LIRMA to detect f ree aε3-CTP. It εhould be poεεible to conεtruct similar LIRMA systems to measure a variety of soluble glycoproteins by employing lectins and antibodies with different carbohydrate and peptide binding specificiti eε. Aror et al . have reported the presence of two different molecules with as3 CTP immunoreactivi ty in choriocarcinoma urine uεing the R141 RIA (6) . The iarger form had a G-100 elution volume εimilar to that of native hCG whereas the smaller form had an elution volume εimilar to that of to free aεialo 3CTP. These desialylated forms of hCG were not detectable in serum from the same patientε. The preεent εtudy confirmε the findingε of Amr et al . ( 6) uεing improved methodologieε which can be appl ied to the routine detection of theεe aεialo formε of hCG Table 3 Characteristics of Lectin-Immunoradiometric Assays for Desialylated Forms of hCG Desialylated Radiolabeled Method* Agarose Linked Lectin Molecules Detected** Antibody A PNA ashCG, ashCGβ , ashCG3-CTP 125 I-R525 I α> CM B PNA ashCG, ashCGβ 125 I-B105 I C PNA ashCG 125 I _ B i07 D RCA ashCG, ashCGβ 1 25 I-R525 * Dose response curves for methods A-D are shown in Figure 2 in panels A-D, respectively. **Methods A-C and method D detect molecules with desialylated 0-serine linked and desialylated asparagine linked oligosaccharides, respectively. in clini cal εpeci enε. Two peaks of immunoreactivity were present in the Sephadex G-100 el ution prof ile of urine from a patient with choriocarcinoma. Analyses of the first peak with the R141 RIA, PNA-R525 LIRMA indicated that approximately 16% of total hCG waε aεialo. In contrast, all of the hCG gCTP immunoreactivity in the second peak could be accounted for by the presence of as CTP reactivity using the R141 RIA and PNA R525 LIRMA. Since this smaller molecule with CTP immunoreactivity was not detectable using the RCA-R525-LIRMA, it seemε likely that it containε only aεialo O-εerine l inked carbohydrate εide chainε. Thiε finding would alεo imply that ashCG but not hCG iε proteolysed to release a smal l peptide from the 3CTP region. Analysiε of the gel filtration profile of a urine concentrate from a normal pregnant woman uεing these asεayε indicated the preεence of only εmall amountε of ashCG reactivity in the area of the elution volume of hCG. Furthermore, the smal l molecule with as CTP reactivity present in the urine from a choriocarcinoma patient waε not detected. These f indings support the proposal of Aror et al . ( 6) that the molecule with characteristicε εimilar to thoεe of free aε ΘCTP may be a specific marker for choriocarcinoma. The PNA-R525 and RCA-R525 LIRMA' ε and the R141 RIA with an extraction εtep have been util ized in conjunction with an IRMA for hCG to determine the concentrationε of aεialo formε of hCG relative to thoεe of total hCG in three different εubject groupε (Table 2) . The mean percentages of asialo hCG over hCG obtained using all three aεεayε were εignificantly higher for urineε from choriocarcinoma patientε than thoεe hydatidiform mole patientε and normal pregnant women. Although the mean val ues for hydatidiform mole patients were also higher than those for normal pregnant women, the differences were not statistically significant. Thiε appearε to be due to the high variance wi thin εubject groupε. Future studies with serially collected specimens from individuals within each subject group will be conducted in order to obtain a better understanding of the utili ty of asialo hCG measurementε in diεcriminating between gestational trophoblasti c disease and normal pregnancy. It is interesting to compare the val ues obtained for asi alo hCG in patients wi th trophoblastic diseases using the different methodε. The val ues obtained using the R141 RIA and PNA-R525 LIRMA. Since the fi rst two methods detect terminal GalS 1 — >3Gal NAc residueε and the laεt method detectε terminal Gai sl— »4Gl cNAc reεidueε , this observation may reflect a greater degree of desialylation of the O-εerine l inked side chains than the N-asparagine l inked side chains in the hCG excreted by these patients . Further studies of the nature of hCG produced by gestational trophoblastic diseaεe patients will be requi red to answer thiε question. The origin of the asialo forms of hCG excreted by trophoblastic diseaεe patients is also not understood. They may be the result of an altered sialylation mechaniεm in trophoblastic tumor tisεue or peripheral desialylation. The improved methods for asialo hCG detection described in this present study εhould be uεeful in examining the mechaniεmε related to the excretion of these unusual forms of hCG. Peferences 1. Kesεler MJ, Reddy MS, Shah RH, Bahl OP, Structureε of N-glycoεidic carbohydrate unitε of human chorionic gonadotropin. J Biol Chem 1979; 254: 7901-7908. 2. Keεεler MJ, Miεe T, Ghai RD, Bahl OP, Structure and location of the O-glycosidic carbohydrate units of human chorionic gonadotropin. J Biol Chem 1979; 254: 7909-7914. 3. Birken S, Canfield RE, Isolation and amino acid sequence of COOH-ter inal frage entε from the beta-εubunit of human choriogonadotropin. J Biol Chem 1977; 252: 5386-5392. 4. Niεhiraura R, Endo Y, Tanabe Y, Ashitaka Y, Tojo S, The biochemical properties of urinary human chorionic gonadotropin from the patients with trophoblastic diseases. J Endocrinol Invest 1981; 4:349-358. 5. Mizucchi T, Niεhimura R, Derappe C, Taniguchi T, Hamamoto T, Machizuki M, Kobata A, Structures of the sugar chains of human chorionic gonadotropin produced in choriocarcinoma. J Biol Chem 1983; 258: 14126-14129. 6. Amr S, Wehmann RE, Birken S, Canfield RE, Nisula BC, Characterization of a carboxtyterminal peptide fragment of the human chorionic gonadotropin beta- subunit excreted in the urine of a woman with choriocarcinoma. J Clin Invest 1983; 71:329-339. 7. Amr S, Rosa C, Wehmann R, Birken S, Nisula B, Unusual molecular forms of hCG in gestational trophoblastic neoplasia. Ann Endocrino Paris: 1984; 45:321-326. 8. Birken S, Canfield, RE, Lauer R, Agosto G, Gabel M, Im uno chemical determinants unique to human chorionic gonadotropin: Importance of sialic acid for antisera generated to human chorionic gonadotropin 3-subunit COOH-terminal peptide. Endocrinology 1980; 106: 1659-1664. 9. Goldεtein IJ, Hayes CE, Lectins: Carbohydrate binding proteins. Adv Carbohydr Chem Biochem 1978; 35:128-340. 10. Cole LA, Birken S, Sutphen S, Husεa RO, Pattillo RA, Abεence of the COOH-terminal peptide on ectopic human chorionic gonadotropin beta-subunit (hCGbeta). Endocrinology 1982; 110:2198-2200. 11. Yamamoto K, Tεuji T, Tarutani 0, A saw a T, Phosphorylated high mannose type and hybrid-type oligoεacchoride chainε of human thyroglobulin isolated from malignant thyroid tisεue, Biochemica and Biophyεica ACTA 1985; 938: 84-91. 12. Del Dillano B, Brennan S, Brock P, Bucher C, Liu V, McClure M, Rake B, Space S, Weεtrick B, Shoemaker H, Zwawski B, Radioimmunometric asεay for a monoclonal antibody-defined tumor marker, CA19-9. Clin. Chem. 1983; 29:549-552. 13. Canfield RE, Morgan FJ, Human chorionic gonadotropin. I. Purification and biochemical characterization. In: Berson SA, Yalow RS, eds: Methods in Investigative and Diagnostic Endocrinology. III. Non-pituitary hormones. Amsterdam: North Holland Publishing Co. 1973; 727-733. 14. Morgan FJ, Canfield RE, Vaitukaitis JL, Roεε GT, Preparation and characterization of the εubunitε of hCG. In: Berεon SA, Yalow RS, eds. Methods in Investigative and Diagnostic Endocrinology. III. Non-pituitary hormones. Amsterdam: North Holland Publishing Co. 1973; 733-742. 15. Morgan FJ, Birken S, Canfield RE, The araino acid sequence of human chorionic gonadotropin: the alpha subunit and beta εubunit. J Biol Chem 1975; 5 250: 5247-5258. 16. Birken S, Canfield R, Agosto G, Lewis J, Preparation and characterization of an improved ° alpha-COOH- terminal immunogen for generation of specific and εenεitive antiεera to human chorionic gonadotropin. Endocrinology 1982; 110: 1555-1563. 5 17. Ehrlich PH, Mouεtafa ZA, Krichevεky A, Birken S, Armstrong EG, Canfield RE, Characterization and relative oreintation of epitopes for monoclonal antibodieε and antiεera to human chorionic 0 gonadotropin. Amer J Repord Immunol Microbiol (in preεs) . 5 18. Fraker PJ, Speck Jr, JC, Protein and cell membrane iodinations with a sparingly soluble chloromide, 1 ,3 ,4 ,6-tetrachloro-3a, 6a-diphenyl-glycouracil . Biochem Biophys Res Comm 1972; 80: 849-857. 0 19. Robard D, Statistical quality control and routine data processing for radioimrounoasεays and immunoradiometric asεayε. Clin Chem 1974; 5 20:1255-1270. 20. Armstrong EG, Ehrlich PH, Birken S, Schlatterer JP, Siris E, Hembree W, Canfield RE, Use of a highly sensitive and specific immunoradioroetric assay for detection of human chorionic gonadotropin in urine of normal, non-pregnant and pregnant individuals. J Clin Endo Metab 1984; 59:867-874. 21. Compariεonε among treatment meanε in an analysis of variance. Agricultural Research Service of the U.S. Department of Agriculture, ARS/H/6.

Claims: What iε claimed iε: 1. A method for determining the presence of a εoluble deεialylated glycoprotein in a biological fluid which compriεes: a) contacting a sample of the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the deεialylated glycoprotein to produce a complex; b) εeparately recovering the resulting complex from the biological fluid; c) contacting the complex so recovered under appropriate conditions with at leaεt one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex; d) detecting the presence of antibody so bound; and e) thereby determining the presence of desialylated glycoprotein in the biological fluid. 2. A method of claim 1, wherein the desialylated glycoprotein is hCG, thyroglobulin, carcinoembryonic antigen or CA19-9. 3. A method of claim 3, wherein the deεialylated glycoprotein iε deεialylated hCG. 4. A method according to claim 3, wherein the antigenic determinant is on the 3 subunit of desialylated hCG. 5. A method according to claim 3, wherein the antigenic determinant is the carboxy-terminal region of the 3 subunit of desialylated hCG. 6. A method of claim 1, wherein the biological fluid is urine, blood, εemen, sali ia or pus. 7. A method of claim 4, wherein the biological fluid is urine. 8. The method of claim 1, wherein the lectin specifically binds to the carbohydrate εtructure GalSl→ 3GalNAc. 9. A method according to claim 8, wherein the lectin compriεeε peanut lectin derived from Arachfrs hypogea. 10. The method of claim 1, wherein the lectin specifically binds to the carbohydrate structure Gal 31-- 4GlcNAc. 11. The method of claim 10 wherein the lectin iε a caεtor bean lectin derived from Ricinus communis. 12. A method according to claim 11, wherein the detectable antibody is radiolabeled or fluorescently labeled. 13. A method according to claim 1, wherein the antibody is a monoclonal antibody. 14. A method for quantitatively determining the amount of a soluble desialylated glycoprotein in a biological fluid which compriseε: a) contacting a sample of the biological fluid with a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a complex; b) separately recovering the resulting complex from the biological fluid; c) contacting the complex so recovered under appropriate conditions with a predetermined amount of at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of selectively binding to glycoprotein present in the complex; d) determining the amount of antibody so bound; and e) thereby determining the amount of desialylated glycoprotein in the biological fluid. 15. A method of claim 14, wherein the desialylated glycoprotein is hCG. 16. A method of diagnosing in a patient a diseaεe εuch aε choriocarcinoma or hydatidiform mole which is associated with the presence of elevated levels of desialylated hCG which compriseε quantitatively determining the amount of deεialylated hCG in a εample of biological fluid from the patient using the method of claim 15, comparing the amount so determined with the amount present in a normal patient and thereby diagnosing the diseas * 17. A method for determining the presence of a εoluble deεialylated glycoprotein in a biological fluid which comprises: a) contacting a sample of the biological fluid with at least one antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of binding to the glycoprotein to produce a complex; b) separately recovering the resulting complex from the biological fluid; c) contacting the complex so recovered with a suitable amount of an appropriate detectable lectin; d) detecting the presence of lectin so bound; and e) thereby determining the presence of desialylated glycoprotein in the biological fluid. 18. A method of claim 17, wherein the detectable lectin is radiolabeled or fluorescently labeled. 19. A method for determining the presence of a soluble deεialylated glycoprotein in a biological fluid which compriseε: a) contacting a εample of the biological fluid with a εuitable amount of an appropriate detectable lectin capable of εelectively binding to the deεialylated glycoprotein to produce a complex; b) contacting the complex under appropriate conditionε with at leaεt one antibody directed to an antigenic determinant on the deεialylated glycoprotein and capable of εelectively binding to glycoprotein in the complex; c) εeparately recovering the reεulting complex from the biological fluid; d) detecting the preεence of lectin bound to the recovered complex; and e) thereby determining the preεence of deεialylated glycoprotein in the biological fluid. 20. A method for determining the presence of soluble desialylated glycoprotein in a biological fluid which comprises: a) contacting a sample of the biological fluid with at least one detectable antibody directed to an antigenic determinant on the desialylated glycoprotein and capable of binding to the glycoprotein to produce a complex; b) contacting the complex with a suitable amount of an appropriate lectin capable of selectively binding to glycoprotein present in the complex; c) separately recovering the resulting complex from the biological fluid; d) detecting the presence of antibody bound to the recovered complex; and e) thereby determining the preεence of deεialylated glycoprotein in the biological fluid. 21. A method for determining the preεence of εoluble . . deεialylated glycoprotein in a biological fluid which comprises: a) subεtantially concurrently contacting a sample of the biological fluid under appropriate conditions with both a suitable amount of an appropriate lectin capable of selectively binding to the desialylated glycoprotein to produce a recoverable complex and at least one detectable antibody directed to antigenic determinant on the deεialylated glycoprotein capable of εelectively binding to the glycoprotein and to the recoverable complex; b) εeparately recovering the reεulting complex from the biological fluid; c) detecting the preεence of antibody so bound to the recovered complex; and d) thereby determining the presence of deεialylated glycoprotein in the biological fluid. 22. A method for determining the preεence of εoluble deεialylated glycoprotein in a biological fluid which comprises: a) substantially concurrently contacting a sample of the biological fluid under appropriate conditions with both an antibody directed to an antigenic determinant on the desialylated glycoprotein to produce a recoverable complex and a εuitable amount of an appropriate detectable lectin capable of selectively binding to desialylated glycoprotein and to the recoverable complex; b) separately recovering the resulting complex from the biological fluid; c) detecting the presence of lectin bound to the recovered complex; and d) thereby determining the presence of desialylated glycoprotein in the biological fluid.

Inventors: ARMSTRONG ELMO G, BIRKEN STEVEN, CANFIELD ROBERT E, COLE LAURENCE A, IMAMURA SADOMI, ARMSTRONG, ELMO, G., BIRKEN, STEVEN, CANFIELD, ROBERT, E., COLE, LAURENCE, A., IMAMURA, SADOMI
Applicants: UNIV COLUMBIA, THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF
Application year: 1986
Main classification: G01N33
IPC codes: G01N33