3-NITROPYRIDINE-DERIVATIVES, PROCESS FOR THEIR PREPARATION AND PHARMACEUTICAL COMPOSITION CONTAINING THEM

Abstract: Substituted 3-nitropyridines are disclosed to have activity in increasing the sensitivity of hypoxic tumor cells to therapeu­ tic radiation. Also disclosed are methods of preparing such compounds by amination of the corresponding chloro-3-nitro­ pyridine with 2,3-dihydroxypropylamine and pharmaceutical compositions including such compounds.

Description: TITLE OF THE INVENTION 3-nitropyridin,e-derivatives, process for their preparation and pharmaceutical composition containing them. BACKGROUND OF THE INVENTION This invention relates to substituted 3-nitropyridine compounds used as sensitizers of hypoxic tumor cells to therapeutic radiation. It also relates to the process of preparing euch compounds by aminating chloro-3-nitropyridines to produce the substituted 3-nitropyridines. At the present time, certain other unrelated compounds are in experimental clinical use as radiation sensitizers. However, these compounds -- for example, metronidazole and misonidazole -- suffer from the drawback that they also cause neurotoxicity which limits their usefulness. The compounds of the present invention are effective radiation sensitizers, and are believed to have a more favorable therapeutic ratio. DETAILED DESCRIPTION OF THE INVENTION The compositions of the present invention are nitropyridine compounds of the formula EMI2.1 wherein the NHCH2CHOHCH2OH group is attached at position 2, 4, or 6 of the pyridine ring. The substituted nitropyridine compounds of the present invention are prepared in the following manner: A (2,4 or 6)-chloro-3-nitropyridine of the formula: EMI2.2 in a suitable solvent such as a lower aliphatic alcohol, or a polar aprotic solvent such as dimethylformamide, dimethylsulfoxide, or others such as tetrahydrofuran, glyme, diglyme, tetramethylurea, chloroform, or methylene chloride is treated with the selected amine reactant of the formula H2NCH2CHOHCH20H, in the presence of sufficient base to neutralize the hydrogen chloride formed. The reaction temperature is not critical and may vary from 0-1000C, preferably from about 0-250C for a period of from 1-24 hours. Suitable bases employed to neutralize the hydrogen chloride formed are tertiary amines such as triethylamine and pyridine. If desired, the neutralizing base may be supplied by using excess amine reactant. Inorganic bases such as alkali metal bicarbonates, carbonates and hydroxides may also be employed. The product is recovered in substantially pure form by removal of solvent by evaporation under reduced pressure and the residue containing the product is chromatographed and crystallized from suitable solvents. The method of treatment of human patients or domestic animals undergoing radiation treatment of malignant disease processes employs the compounds of the present invention in pharmaceutical compositions that are administered orally or intravenously. The dose employed depends on the radiation protocol for each individual patient. In protocols where the radiation dose is divided into a large number of fractions, the drug can be administered at intervals in the schedule and not necessarily with each radiation treatment. It should be noted that the compounds of the present invention are not intended for chronic administration. In general, the drug is administered from 10 minutes to 5 hours prior to the radiation treatment in a dosage amount of between 0.25 to about 4.0 grams per square meter of body surface. The dosage range given is the effective dosage range and the decision as to the exact dosage used must be made by the administering physician based on his judgement of the patient's general physical condition. In determining the dose for the individual patient, the physician may begin with an initial dose of 0.25 g/square meter of body surface to determine how well the drug is tolerated and increase the dosage with each suceeding radiation treatment, observing the patient carefully for any drug side effect. The composition to be administered is an effective amount of the active compound and a pharmaceutical carrier for said active compound. EXAMPLE 1 4- (2, 3-Dihydroxy-l-propylamino) -3-nitropyridine A solution of 4-chloro-3-nitropyridine (1.1 g, 6.94 mmol) and 3-amino-lr2-propanediol (1.22 g, 13.4 mmol) in isopropanol (50 ml) was stirred at 20-250C for 20 hours and then concentrated under reduced pressure. Flash chromatography of the residue over silica gel and elution with 10% MeOH-90% CHC13 gave pure 4- (2 ,3-dihydroxy-l-propylamino) -3- nitropyridine (500 mg, 33.8g). An analytically pure sample, m.p. 131-350C, was obtained upon recrystallization from MeOH-EtOAc-hexane. EXAMPLE 2 2- (2, 3-Dihydroxy-l-propylamino) -3-nitropyridine A solution of 2-chloro-3-nitropyridine (3.22 g, 20.3 mmol), 3-amino-1,2-propanediol (1.85 g, 20.3 mmol) and triethylamine (2.05 g, 20.3 mmol) in isopropanol (80 mL) was stirred at 20-250C for 18 hours and then at reflux for 6 hours. After concentrating under reduced pressure, the residue was flash chromatographed over silica gel. Elution with 5% MeOH-95% CHIC13 and recrystallization from EtOAc-hexane afforded analytically pure product (2.64 g, 61%), m.p. 95.5-98.0 C. EXAMPLE 3 The procedure of Example 2 was repeated using 3-nitro-6-chloropyridine in place of 2-chloro3-nitropyridine to give 6-(2,3-dihydroxy-l-propylamino)-3-nitropyridine.

Claims: CLAIMS: 1. 3-nitropyridine compounds of the formula I EMI6.1 wherein the NHCH2CHOHCH2OH substituent is attached at position 2, 4 or 6, or salts of the compounds of formula I. 2. A compound according to claim 1 which is 4-(2,3-dihydroxy-1-propylamino)-3-nitropyridine. 3. A compound according to claim 1 which is 2-(2,3-dihydroxy-1-propylamino)-3-nitropyridine. 4. Process for the preparation of the 3-nitropyridine compounds of formula I EMI6.2 according to claim 1, characterized inthat a 3-nitro- pyridine, having in the position 2 or the position 4 or the position 6 a leaving group is reacted with an amine of formula II H2NCH2CHOHCH20H and the resulting product of formula I is isolated in the free form or as salt thereof. 5. Process according to claim 4, characterized in that in the starting material the leav@@- ing group is a haloatom, preferably chloride, and t.lt: the reaction is performed in the presence of a ba3(?4 6. Pharmaceutical composition for enhancing the therapeutic effect of radiation, characterized in that it contains as pharmaceutically effective ingredient a compound of formula I according to claim 1 or a pharmaceutically acceptable salt thereof. 7. Pharmaceutical composition according to claim 6, characterized in that it furthermore contains a pharmaceutically acceptable carrier. 8. Pharmaceutical composition according to claim 6 or 7, characterized in that it contains as pharmaceutically effective ingredient a compound according to claim 2 or 3.

Inventors: SAARI, WALFRED S.
Applicants: MERCK & CO. INC.
Application year: 1986
Main classification: C07D213
IPC codes: A61K31, A61P35, C07D213