BERBAN DERIVATIVES AND THEIR PREPARATION AND PHARMACEUTICAL FORMULATION

Abstract: The invention relates to novel racemic or optically active berban derivatives of the general formula (I), wherein R¹ and R² represent independently from the other a hyd­ roxyl, straight or branched chain alkoxy group having 1 to 6 carbon atoms or R¹ and R² together represent a C₁₋₃ alkylene­ dioxy group; R³ and R⁴ represent independently from the other hyd­ rogen, straight or branched chain alkyl group, having 1 to 4 carbon atoms and optionally substituted by hydroxyl group, or a C₂₋₆ alkoxycarbonyl or cyano group; and R⁵ represents hydrogen, straight or branched chain alkyl group having 1 to 6 carbon atoms, C₁₋₇ aliphatic or aromatic acyl group or C₁₋₇ alkylsulphonyl or arylsulphonyl group and a salt thereof, to pharmaceutical compositions containing them, to the use as well as to process for preparing the novel compounds. The compounds of the general formula (I) are selective α₂-adrenergic receptor-blocking substances and thus, they may be used for the treatment of depressive illnesses.

Description: The invention relates to novel berban derivatives, to pharmaceutical compositions containing them, to the use as well as to a process for preparing the novel compounds. It has been known for a long time that the alkaloids of the Rauwolfia plant family and their analogues with a berban skeleton are biologically active and possess in some cases valuable pharmacological activity. Antiinflammatory [Acta. Chim. Acad. Sci. Hung. 100 , 1 (1979)], prostaglandin-like and prostaglandin antagonist substances [Chem. Bar. 109 , 3390 (1976)] as well as antihypertensive compounds [J. Med. Chem., 27 , 1411 (1984)] have been found among the above derivatives. It is known that the noradrenergic part of the central nervous system plays an important role in the antidepressive action of some compounds [Trends in Pharm. Sci., 1982 , 314]. The concentration of norepinephrine (noradrenaline) in the synaptic space is increased under the effect of such compounds. The feed-back inhibition of the norepinephrine release is suspended by the α₂-adrenergic antagonists thus, the release of norepinephrine is enhanced. Therefore, a very important therapeutical demand can be satisfied by the α₂-antagonists which consists in that they exert a rather weak inhibition on the postsynaptic α₁-adrenergic receptors and thus, their selectivity against the α₂-receptors is high. It has been aimed to prepared therapeutically useful novel compounds which are more selective than the α₂-antagonists known so far. Thus, the invention relates to new racemic or optically active berban derivatives of the general formula (I) wherein R¹ and R² represent independently from each other a hydroxyl or straight or branched chain alkoxy group having 1 to 6 carbon atoms or R¹ and R² together represent a C₁₋₃ alkylenedioxy group; R³ and R⁴ represent independently from each other hydrogen, a straight or branched chain alkyl group having 1 to 4 carbon atoms and optionally substituted by a hydroxy group, or a C₂₋₆ alkoxycarbonyl or cyano group with the proviso that when R¹ and R² are both methoxy groups, then R³ and R⁴ are not cyano, methoxycarbonyl or hydroxymethyl groups and R³ and R⁴ are not both hydrogen atoms; and R⁵ represents hydrogen, a straight or branched chain alkyl group having 1 to 6 carbon atoms, C 1-7 aliphatic or aromatic acyl group or C 1-7 alkylsulphonyl or arylsulphonyl group and the salts thereof. The meanings of these substituents are always the same in this description and will not be repeated below. An other embodiment of the invention is to provide a process for the preparation of the new compounds of general formula (I) and the salts thereof, which comprises reducing a racemic or optically active oxo compound of the general formula (II) , wherein R¹, R², R³ and R⁴ are the same as above, and optionally resolving, alkylating or acylating the obtained compound of general formula (I) in a known method. For the preparation of the acid addition salts of the compounds of general formula (I), organic and inorganic acids such as tartaric or citric acid and hydrochloric or sulphuric acid may be used. The reduction of the compounds of the general formula (II) can be carried out by using metal hydrides, e.g. sodium borohydride, lithium aluminium hydride or lithium tris(2-butyl)-borohydride or by using hydrogen in the presence of a catalyst, or by the means of sulphur-containing substances, aluminium alkoxides, nascent hydrogen or by using other reagents which are useful to saturate the carbon-oxygen double bond. In the course of preparing the compounds of general formula (I), the nature of the selected reducing agent influences the ratio of the stereoisomers containing the C₁₄ hydroxyl group in the equatorial or axial position. For example when the space demand of the metal hydride used for the reduction is enhanced, then the ratio of the isomer containing the C₁₄ hydroxyl group in the axial position is increased in the product obtained. Using sodium borohydride for the reduction, the isomer containing the C₁₄ hydroxyl group in the axial position amounts to 80 %. When using lithium tris(2-butyl)-borohydride, the product obtained contains nearly exclusively the stereoisomer containing the C₁₄ hydroxyl group in the axial position. When carrying out the reduction with a system consisting of an alkaline metal and an alcohol, i.e. by using nascent hydrogen, the thermodynamically favourable derivative containing the hydroxyl group in the equatorial 14α-position is obtained. The racemic or optically active compounds of general formula (II) can be prepared by using methods known from the literature [Chem. Ber., 109 , 1724 (1976); and J. Med. Chem. 27 , 1411 (1984)]. On investigating the compounds of general formula (I), it has been found that they show a substantially more selective antagonism against the α₂-adrenergic receptors as compared to the substances known so far [yohimbine, phentolamine, idazoxan, imiloxan] [see, Drugs of the Future, 10 , 841 (1985) and the references cited therein]. The compounds of general formula (I) show a very high selectivity concerning the blocking effect on the presynaptic α₂- and postsynaptic α₁-adrenergic receptors, respectively. The compounds of general formula (I) have a very favourable spectrum of activity and do not exert any antagonistic effect on the dopamine, serotonine, histamine or muscarine receptors; in addition, their oral absorption is very good. The substances described in the Examples 3, 24 and 25 are particularly preferably representatives of these compounds. The above statements are based on the following test-results: Method of the investigation The tissues were suspended at 37 °C in an organ bath containing 5 ml of Krebs solution. The stimulation was achieved according Field's method by using rectangular impulses (with a frequency of 0.1 Hz, supramaximal potential and an impulse duration of 1 ms). Both the compounds of general formula (I) and the reference substances were tested against xylazine (on the rat vas daferens) as well as against epinephrine (on the longitudinal smooth muscle preparation from the guinea pig ileum). The amplitude of the contraction response caused by the stimulation is inhibited on the α₂-adrenergic receptors by xylazine or norepinephrine. This effect is abolished by the blocking substances. The pA₂ value of the blocking substances was calculated by the means of the negative logarithm of the molar concentration of the blocking substances establishing a dose ratio of 2 [Arunlakshana and Schild: Br. J. Pharmacol., 14 , 48 (1959)]. The nature of the competition was similarly studied on the Schild's curve: it was statistically analyzed if the slope of the curve were significantly different from 1. When it is significantly different from 1, then the antagonism is non-competitive (NC); when it is not significantly different from 1, then the antagonism is competitive (C). The ratio of the selective α₂-inhibiting effect of the compounds of the general formula (I) to that of yohimbine was expressed by the relative activity. Method of the investigation Phenylephrine was used as agonist on the α₁-adrenergic receptors whereby both the rat vas deferens and the pulmonary artery of the rabbit were contracted. This concractile effect was inhibited by antagonists. The pA₂ value was determined by using the method of Arunlakshana and Schield (1959) cited above. The nature of the competition is also given (NC means non-competitive, C means competitive). Method of the investigation On the α₁-receptors of the pulmonary artery, a contraction is elicited by norepinephrine which is inhibited by antagonists. The pA₂ values and the nature of the competition were determined by using the method of Arunlakshana and Schild (1959) cited above (NC means non-competitive; C means competitive). Method of the investigation The selectivity values were calculated on the basis of Tables 1 and 2. The pA₂ values concerning the α₁-receptor of the antagonists were divided by the pA₂ values concerning the α₂-receptor of the antagonists (α₁/α₂). The higher is this ratio, the more selective is the given compound. The low ratio of yohimbine, thought to be a selective antagonist, is surprising. Method of the investigation Male rats with 100 g of body-weight were intraperitoneally pre-treated (at minute 0) with 320 mg/kg of α-methyl-p-tyrosinc. The animals of the control group as well as the group treated with the compounds of the general formula (I) 4 animals in each group) were killed it, the 30th, 60th, 90th and 120th minute, respectively, and the frontal cortex and the striatum were prepared and homogenized in perchloric acid of 0.2 mole/litre concentration. After centrifuging, the norepinephrine content (frontal cortex) or the dopamine content (striatum) of the supernatant, respectively, was determined by using the method of the intense liquid chromatography-electrochemical detection. The fractional rate constant of the catecholamine development (k f ) and the catecholamine turnover rate (TR CA ) in nmole/g x h⁻¹ as well as the catecholamine turnover time in hours (T t ) were determined by the means of the method of Brodil et al. (1966). The significance level of the difference related to the control group was also determined (** p < 0.02; *** p < 0.001). The pharmaceutical compositions containing the compounds of the general formula (I) may be prepared according to known methods, by using one or more representatives of compounds of the general formula (I) together with additives commonly used in the pharmaceutical industry. The thus-prepared pharamaceutical compositions may be administed orally, parenterally, intravenously or intramuscularly. Preferred forms of the pharmaceutical compositions are the tablets, dragées, capsules, powder mixtures, aqueous suspensions, solutions or injectable solutions. The active ingredient content of the compositions may be varied under wide limits (from 0.005 to 90 %) For the treatment of depressions, the compounds of the general formula (I) may preferably be used in a daily dose of 5 to 20 mg administered at once or divided in several subdoses. The above dose range is only informative although this may be varied according to the exercise of the physician's discretion. The invention is illustrated by the following non-limiting Examples. Example 1 Preparation of methyl 14-β-hydroxy-7,8-methylenedioxy-alloberban-13-carboxylate 10 g (0.26 mole) of sodium borohydride are portionwise added to a solution containing methyl 7,8-methylenedioxy-14-oxoalloberban-13-carboxylate (0.1 mole) in 80 ml of dichloromethane and 200 ml of methanol while stirring and cooling by ice during 1 hour. After ending the addition, the mixture is stirred for additional half hour, then 7 ml of glacial acetic acid are dropped in and evaporated to dryness. The residue is rubbed with water, alkalinized to pH 8 by adding 5 % aqueous sodium carbonate solution and extracted with dichloromethane. The dichloromethane phase is evaporated and the residue is recrystallized from methanol to give the title product in a yield of 71 %, m.p.: 159-162 °C. Analysis : Calculated for C₂₀H₂₅NO₅ (molecular weight 359.4) C % = 66.83; H % = 7.01; N % = 3.90 %; Found: C % = 66.71. H % = 7.07, N % = 3.71. IR (KBr): 3500 (OH) 2750-2800 (Bohlmann band), 1700 cm⁻¹ (COOCH₃) ¹H-NMR (CDCl₃, δ): 5.60; 6.62 (2H, s, C₆-H, C₉-H, 5.85 (2H, s, OCH₂O), 4.25 (1H, m, C₁₄-H), 3.82 (3H, s, COOCH₃). Example 2 Preparation of methyl 14β-hydroxy-7,8-methylenedioxy-alloberban-15-carboxylate 0.1 mole of 7,8-methylenedioxy-14-oxoalloberban-15-carboxylate is reduced as described in Example 1 to give the title product in a yield of 69 %, m.p.: 148-150 °C. Analysis : Calculated for C₂₀H₂₈NO₅ (molecular weight 359.4) C % = 66.83; H % = 7.01, N % = 3.90; Found: C % = 66.81, N % = 7.22, N % = 3.72. IR (KBr): 3460 (OH), 2750-2800 (Bohlmann band), 1700 cm⁻¹ ₈(COOCH₃) ¹H-NMR (CDCl₃, δ): 6.50, 6.72 (2H, s, C₆-H, C₉-H) 5.84 (2H, s, OCH₂O) 4.25 (1H, m, C₁₄-H) 3.68 (3H, s, COOCH₃) Example 3 Preparation of 14β-hydroxy-7,8-methylenedioxyalloberban 0.1 mole of 7,8-methylenedioxy-14-oxoalloberban is reduced as described in Example 1 and the product obtained is recrystallized from 300 ml of methanol to give the title compound in a yield of 59.8 %, m.p.: 172-175 °C (after recrystallization from ethanol). The hydrochloride melts at 232-236 °C (after recrystallization from methanol). Analysis : Calculated for C₁₈H₂₃NO₃ (molecular weight 301.4) C % = 71.73, H % = 7.68, N % = 4.61; Found: C % = 71.77, H % = 7.70, N % = 4.58. MS (m/e): 301 (6), 300 (100), 209 (4,2), 284 (6), 282 (1.9), 272 (2.5), 270 (5.6), 260 (0.7) 258 (1), 256 (0.8), 244 (1.2), 242 (3), 240 (1.2), 230 (2.8), 228 (9.6), 226 (2.2), 216 (5.4), 214 (6), 202 (3), 189 (23), 175 (29), 174 (14). IR (KBr): 3460 (OH) 2750-2800 cm⁻¹ (Bohlmann-band) ¹H-NMR (CDCl₃, δ): 6.50, 6.72 (2H, s, C₆-H, C₉-H), 5.85 (2H, s, OCH₂O) 4.05 (1H, m, C₁₄-H). The methanolic mother liquor is concentrated and the residue is subjected to flashchromatography on KG-PF₂₅₄ adsorbent by using a 14:1 mixture of benzene/methanol under a pressure of 1.8 atmospheres. A 5 % yield of the title product is obtained, further on 14α-hydroxy-7,8-methylenedioxyalloberban is obtained in a yield of 14.8 %, m.p.: 214-217 °C (after recrystallization from methanol). Analysis Calculated for C₁₈H₂₃NO₃ (molecular weight 301.4) C % = 71.73, H % = 7.68, N % = 4.61; Found: C % = 71.70, H % = 7.69, N % = 4.52. MS (m/e): 301 (60),300 (100), 284 (6.7), 272 (2.2), 260 (0.6), 242 (2.7), 230 (3.0), 228 (12) 216 (7.5), 214 (6.5), 202 (3.1), 190 (14), 189 (29), 176 (13), 175 (33), 174 (18). IR (KBr): 3450 (OH), 2750-2850 cm⁻¹ (Bohlmann-band) ¹H-NMR (CDCl₃, δ): 6.52, 6.68 (2H, s, C₆-H, C₉-H) 5.86 (2H, s, OCH₂O), 3.75 (1H, m, C₁₄-axH) Example 4 Preparation of (+)-(1R, 12S, 14S, 17R)-14-hydroxy-7,8-methylenedioxyalloberban 0.1 mole of (+)-(1R, 12S, 17R)-7,8-methylanedioxy-14-oxoalloberban is reduced as described in Example 1 to give the title compound in a yield of 61 %, [α] 20 / D = +172° (c = 1, dichloromethane), m.p.: 185 °C (after recrystallization from ethanol). Example 5 Preparation of (-)-(1S, 12R, 14R, 17S)--14-hydroxy-7,8-methylenedioxyalloberban 0.1 mole of (-)-(1R, 12S, 17R)-7,8-methylenedioxy-14-oxoalloberban is reduced as described in Example 1 to give the title compound in a yield of 57 %, m.p.: 185 °C (after recrystallization from ethanol), [α] 20 / D = -165° (c = 1, dichloromethane). Example 6 Preparation of methyl 14α-hydroxy-7,8-methylenedioxyberban-13-carboxylate 0.1 mole of methyl 7,8-methylenedioxy-14-oxoberban-13-carboxylate is reduced as described in Example 1 to give the title compound in a yield of 68 %, m.p.: 160-166 °C (after recrystallization from methanol). (This is a mixture of the C 13ax -COOCH₃ and C 13eq -COOCH₃). Analysis : Calculated for C₂₀H₂₅NO₅ (molecular weight 359.4) C % = 66.83, H % = 7.01, N % = 3.90 %; Found: C % = 66.89, H % = 7.11, N % = 3.90 %. IR (KBr) 3480 (OH), 1720 (C 13ax -COOCH₃) 169 cm⁻¹ (C 13eq -COOCH₃) ¹H-NMR (CDCl₃, δ): 6.55, 6.65 (2H, s, C₆-H, C₉-H), 4.85 (2H, s, OCH₂O) 420 (1H, m, C₁₄-H) 3.81, 376 (3H, s, 1/3 COOCH₃ + 2/3 COOCH₃) Example 7 Preparation of methyl 14α-hydroxy-7,8-methylenedioxyberban-15-carboxylate and methyl 14β-hydroxy-7,8-methylenedioxyberban-15-carboxylate 0.1 mole of methyl 7,8-methylenedioxy-14-oxoborban-15-carboxylate is reduced as described in Example 1 and the product obtained is separated by using flash chromatography on a Kieselgel-G adsorbent with a 10:1 solvent mixture of chloroform with methanol under a pressure of 1.8 atmospheres. The first title product is obtained in a yield of 21 %, m.p.: 185-187 °C (after recrystallization from methanol). Analysis : Calculated for C₂₀H₂₅NO₅ (molecular weight 359.4) C % = 66.83, H % = 7.01, N % = 3.90 %; Found: C % = 66.22, H % = 7.17, N % = 3.97 %. IR (KBr): 3350 (OH) 2750-2800 (Bohlmann band), 1710 cm⁻¹ (COOCH₃) ¹H-NMR (CDCl₃, δ): 6.50, 6.70 (2H, s, C₆H, C₉-H) 5.84 (2H, s, OCH₂OH) 4.35 (1H, m, C₁₄-H) 3.70 (3H, s, COOCH₃) The second title product is obtained in a yield of 28 %, m.p.: 205 °C (after recrystallization from methanol). Analysis : Calculated for C₂₀H₂₅NO₅ (molecular weight 359.4) C % = 66.83, H % = 7.01, N % = 3.90; Found: C % = 67.10, H % = 6.99, N % = 3.67. IR (KBr): 3400 (OH), 2750-2850 (Bohlmann band), 1710 cm⁻¹ (COOCH₃) ¹H-NMR (CDCl₃, δ): 6.52, 6.70 (2H, s, C₆-H, C₉-H), 5.85 (2H, s, O-CH₂O) 3.85 (1H, m, C₁₄-H) 3.71 (3H, s, COOCH₃) Example 8 Preparation of 14β-hydroxy-7,8-methylenedioxyberban 0.1 mole of 7,8-methylenedioxy-14-oxoberban is reduced as described in Example 1 to give the title compound in a yield of 70 %, m.p.: 217-219 °C (after recrystallization from methanol). Analysis : Calculated for C₁₈H₂₃NO₃ (molecular weight 301.4) C % = 71.73, H % = 7.69, N % = 7.61; Found: C % = 71.10, H % = 7.60, N % = 4.57. IR (KBr): 3350 (OH), 2750-2800 cm⁻¹ (Bohlmann band) ¹H-NMR (CDCl₃, δ): 6.55, 6.75 (2H, s, C₆-H, C₉-H) 5.85 (2H, s, OCH₂O) 3.65 (1H, m, C₁₄-H) Example 9 Preparation of methyl 14β-hydroxy-7,8-methylenedioxy-epialloberban-13-carboxylate 0.1 mole of methyl 7,8-methylenedioxy-14-oxo-epialloberban-13-carboxylate is reduced as described in Example 1 to give the title compound in a yield of 37 % (after separation by chromatography), m.p.: 202-203 °C. Analysis : Calculated for C₂₀H₂₅NO₅ (molecular weight 359.4) C % = 66.83, H % = 7.01, N % = 3,90; Found: C % = 66.81, H % = 7.22, N % = 3.94. IR (KBr): 3450 (OH), 2750-2850 (Bohlmann band), 1730 cm⁻¹ (COOCH₃) ¹H-NMR (CDCl₃, δ): 6.55, 6.60 (2H, s, C₆-H) 5.85 (2H, s, OCH₂O) 4.2 (1H, m, C₁₄-H) 3.80 (3H, s, COOCH₃) Example 10 Preparation of 14α-hydroxy-7,8-methylenedioxy-epialloberban 0.1 mole of 7,8-methylenedioxy-14-oxo-epialloberban (0.1 mole) is reduced as described in Example 1 to give the title compound in a yield of 49 %. The hydrochloride melts at 212-216 °C (after recrystallization from methanol). Analysis : Calculated for C₁₈H₂₄ClNO₃ (molecular weight 337.84): C % = 63.99, H % = 7.16, N % = 4.15; Found: C % = 64.24, H % = 7.02, N % = 4.00. IR (KBr): 3350 (OH), 2750-2850 (Bohlmann band), 2500-2600 cm⁻¹ (salt) ¹H-NMR (CDCl₃, δ): 6.50, 6.65 (2H, s, C₆-H) 5.85 (OCH₂O) 4.10 (1H, m, C₁₄-H) Example 11 Preparation of 14β-hydroxy-7,8-diethoxyalloberban 0.1 mole of 7,8-diethoxy-14-oxoalloberban is reduced as described in Example 1 to give the title compound in a yield of 61 %, m.p.: 135-139 °C (after recrystallization from methanol). The hydrochloride melts at 159-162 °C (after recrystallization from methanol). Analysis: Calculated for C₂₁H₃₁NO₃ (molecular weight 345.47) C % = 73.00, H % = 9.04, N % = 4.06; Found: C % = 72.69, H % = 9.15, N % = 3.97. IR (KBr): 3390 (OH), 2750-2800 cm⁻¹ (Bohlmann band) ¹H-NMR (CDCl₃, δ): 6.70, 6.60 (2H, s, C₆-H, C₉-H) 4.05 (2H, q, COOC H ₂CH₃) 1.40 (3H, t, COOCH₂C H ₃) Example 12 Preparation of 14β-hydroxy-13-hydroxymethyl-7,8-methylenedioxyalloberban 0.1 m mole of 13-hydroxymethyl-7,8-methylenedioxy-14-oxoalloberban dissolved in 300 ml methanol is stirred at room temperature, then 0.52 mole of sodium borohydride are portionwise added to the mixture during about 1 hour. The mixture is stirred at 40 °C for additional 3 hours, than the pH value is adjusted to neutral by adding 14 ml of glacial acetic acid and the mixture is evaporated to dryness. The residue is triturated with water and alkalinized to pH 8 by adding 5 % aqueous sodium carbonate solution. After filtration, the product is dissolved in 10 ml of methanol, the pH value is acidified to 3 by adding methanolic hydrogen chloride and the thus-obtained product is crystallized to give the hydrochloride of the title compound in a yield of 47 %, m.p.: 243-245 °C. Analysis : Calculated for C₁₉H₂₆ClNO₄ (molecular weight 367.86) C % = 62.03, H % = 7.12, Cl % = 9.64, N % = 3.81; Found: C % = 62.90, H % = 7.27, Cl % = 9.02, N % = 3.62. IR (KBr): 3350 (OH), 2750-2800 (Bohlmann band), 1500 cm⁻¹ (aromatic) Example 13 Preparation of 14β-hydroxy-15-hydroxymethyl-7,8-methylenedioxyalloberban 0.1 mole of 15-hydroxymethyl-7,8-methylenedioxy-14-oxoalloberban is reduced as described in Example 12 to give the title compound as the base in a yield of 41.5 %, m.p.: 152-155 °C (after recrystallization from methanol). Analysis : Calculated for C₁₉H₂₅NO₄ (molecular weight 331.4) C % = 68.86, H % = 7.60, N % = 4.23; Found: C % = 68.12, H % = 7.87, N % = 4.00. IR (KBr): 3400 (OH), 2750-2850 (Bohlmann band) 1500 cm⁻¹ (aromatic). Example 14 Preparation of 14β-hydroxy-13-hydroxymethyl-7,8-methylenedioxyberban 0.1 mole of 13-hydroxymethyl-7,8-methylenedioxy-14-oxoberban is reduced as described in Example 12 to give the hydrochloride of the title compound in a yield of 42 %, m.p.: 245-250 °C. Analysis : Calculated for C₁₉H₂₆ClNO₄ (molecular weight 367.86): C % = 62.03, H % = 7.12, Cl % = 9.64, N % = 3.81; Found: C % = 61.55, H % = 7.70, Cl % = 9.01, N % = 3.29. IR (KBr): 3320, 3400 (OH), 2750-2850 (Bohlmann band), 1490 cm⁻¹ (aromatic). Example 15 Preparation of 7,8-dihydroxy-14β-hydroxyalloberban 1.0 mmole of 14β-hydroxy-7,8-methylenedioxyalloberban is dissolved in 50 ml of dry dichloromethane, 6 mmoles of boron tribromide are dropped to the solution and stirred for 1 day, then evaporated to dryness. The residue is triturated with acetone, filtered and the precipitate is recrystallized from ethanol to give the hydrobromide of the title compound in a yield of 47 %, m.p.: 202 °C (after recrystallization from ethanol). Analysis : Calculated for C₁₇H₂₄BrNO₃ (molecular weight 370.29) C % = 55.14, H % = 6.53, N % = 3.78; Found: C % = 54.81, H % = 6.87, N % = 3.27. IR (KBr): 3400 (OH), 2750-2850 (Bohlmann band), 1480 cm⁻¹ (aromatic). Example 16 Preparation of 7,8-diethoxy-14-oxoalloberban 10 mmoles of methyl 7,8-diethoxy-14-oxoalloberban-13-carboxylate [J. Med. Chem. 27 , 1411 (1984)] and 100 ml of 10 % aqueous hydrochloric acid are heated together at 100 °C for 5 hours. After cooling, 100 ml of water are added to the reaction mixture which is then alkalinized to pH 9 by adding 20 % aqueous sodium hydroxide solution and extracted with dichloromethane. The organic phase is evaporated and the residue is recrystallized from methanol to give the title compound in a yield of 91 %, m.p.: 148-152 °C (after recrystallization from methanol). Analysis : Calculated for C₂₁H₂₉NO₃ (molecular weight 343.45) C % = 73.43, H % = 8.51, N % = 4.08; Found: C % = 73.10, H % = 8.82, N % = 3.71. IR (KBr): 2750-2800 (Bohlmann band), 1700 cm⁻¹ (CO). Example 17 Preparation of ethyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate 0.1 mole of ethyl 7,8-methylenedioxy-14-oxoalloberban-13-carboxylate is reduced as described in Example 1 to give the title compound in a yield of 81 %, m.p.: 149-152 °C (after recrystallization from ethanol). Analysis : Calculated for C₂₁H₂₇NO₅ (molecular weight 373.44) C % = 67.54, H % = 7.29, N % = 3.75; Found: C % = 67.37, H % = 7.31, N % = 3.74. IR (KBr): 3450 (OH), 2750-2800 (Bohlmann band), 1700 cm⁻¹ (COOC₂H₅). Example 18 Preparation of 3β-(β-cyanoethyl)-2β-ethoxycarbonylmethyl-1,2,3,4,6,7-hexahydro-9,10-methylenedioxy-11b(H)-benzo(a)quinolizine A solution containing 2.7 mmoles of 3β-(β -oyanoethyl)-2-ethoxycarbonylmethylene-1,2,3,4,6,7-hexahydro-9,10-methylenedioxy-11b(H)-benzo(a)-quinolizine [Chem. Ber. 109 , 1724 (1976)] in 20 ml of methanol is hydrogenated in the presence of 0.5 g of palladium-on-carbon catalyst. After reduction, the catalyst is filtered out, the filtrate is evaporated to dryness and the residue is recrystallized from ethanol to give the title compound in a yield of 0.72 g (72 %), m.p.: 97- 97.5 °C. Analysis : Calculated for C₂₁H₂₆N₂O₄ (molecular weight 370.44): C % = 68.09, H % = 7.08, N % = 7.56; Found: C % = 68.25, H % = 7.07, N % = 7.52. IR (KBr): 2290 (C≡N), 1730 cm⁻¹ (C=O) ¹H-NMR (CDCl₃, δ): 6.68, 6.57 (2H, s, C₈-H, C₁₁-H) 5.89 (2H, s, OCH₂) 4.21 (2H, q, COOC H ₂CH₃) 1.29 (3H, t, COOCH₂C H ₃) Example 19 Preparation of 15-cyano-7,8-methylenedioxy-14-oxo-alloberban 2.7 mmoles of the product prepared as described in Example 18 is dissolved in 10 ml of benzene, 5.35 mmoles of potassium tertiary-butoxide is added and the mixture is boiled under reflux for 30 minutes. Then, the mixture is cooled to room temperature, neutralized by adding acetic acid and evaporated to dryness. The residue is triturated with 2.5 % aqueous sodium carbonate solution, filtered and the precipitate is recrystallized from methanol to give the title compound in a yield of 0.7 g (80 %), m.p.: 245-248 °C (after recrystallization from methanol). Analysis : Calculated for C₁₉H₂₀N₂O₃ (molecular weight 324.37): C % = 70.42, H % = 6.22, N % = 8.65; Found C % = 70.11, H % = 6.23, N % = 8.52. IR (KBr): 2750-2800 (Bohlmann band), 223o (C≡N), 1700 cm⁻¹ (C=O). Example 20 Preparation of 15-cyano-14β-hydroxy-7,8-methylenedioxyalloberban 0.01 mole of 15-cyano-7,8-methylenedioxy-14-oxoalloberban is reduced as described in Example 1 to give the title compound in a yield of 87 %, m.p.: 225-226 °C (after recrystallization from methanol). Analysis : Calculated for C₁₉H₂₂N₂O₃ (molecular weight 326.39) C % = 69.91, H % = 7.80, N % = 8.58; Found: C % = 69.79, H % = 6.92, N % = 8.41. IR (KBr): 3450 (OH), 2700-2750 (Bohlmann band), 2230 cm⁻¹ (C≡N). Example 21 Preparation of 14β-acetoxy-7,8-methylenedioxyalloberban 6.4 mmoles of acetyl chloride is dropped to a solution containing 1 mmole of 14β-hydroxy-7,8-methylenedioxyalloberban in 10 ml of dichloromethane and the thus-obtained solution is set aside for 1 day. Then the mixture is evaporated to dryness, the residue is triturated with 2.5 % aqueous sodium carbonate solution, extracted with dichloromethane and the organic phase is evaporated to dryness. The residue is recrystallized from methanol to give the title compound in a yield of 92 %, m.p.: 153 °C (after recrystallization from methanol). Analysis : Calculated for C₂₀H₂₅NO₄ (molecular weight 343.41) C % = 69.95, H % = 7.34, N % = 4.08; Found: C % = 69.71, H % = 7.34, N % = 4.01. IR (KBr): 2750-2850 (Bohlmann band), 1720 cm⁻¹ (CH₃CO). ¹H-NMR (CDCl₃, δ): 6.69, 6.53 (2H, s, C₆-H, C₉-H) 5.86 (2H, s, OCH₂O) 5.03 (1H, m, C₁₄-eqH) 1.90 (3H, s, CH₃CO) Example 22 Preparation of 14α-acetoxy-7,8-methylenedioxyalloberban 1.0 mmole of 14α-hydroxy-7,8-methylenedioxyalloberban is acylated as described in Example 21 to give the title compound in a yield of 89 %, m.p.: 155 °C (after recrystallization from methanol). Analysis : Calculated for C₂₀H₂₅NO₄ (molecular weight 343.41): C % = 69.95, H % = 7.34, N % = 4.08; Found C % = 70.02, H % = 7.35, N % = 4.05. IR (KBr): 2750-2850 (Bohlmann band), 1720 cm⁻¹ (CH₃CO) ¹H-NMR (CDCl₃, δ): 6.66 , 6.52 (2H, s, C₆-H, C₉-H) 5.84 (2H, s, OCH₂O) 4.86 (1H, m, C₁₄-axH) 1.96 (3H, s, CH₃CO) Example 23 Preparation of ethyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate A solution containing 1 mole of methyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate and 3 mg of p-toluenesulphonic acid in 5 ml of ethanol is boiled for 5 hours, then evaporated to its one-third volume and the precipitate is filtered. The title compound is obtained in a yield of 59 %, m.p.: 142-146 °C (after recrystallization from ethanol). Analysis : Calculated for C₂₁H₂₇NO₅ (molecular weight 373.4) C % = 67.54, H % = 7.29, N % = 3.75; Found: C % = 67.27, H % = 7.32, N % = 3.49. IR (KBr): 3480 (OH), 2750-2800 (Bohlmann band), 1710 cm⁻¹ (COOCH₃). Example 24 Preparation of 14β-hydroxy-7,8-methylenedioxyalloberban 0.5 g of 7,8-methylenedioxy-14-oxoalloberban (1.67 mmoles) is dissolved in 80 ml of methanol and after adding 0.2 g of Raney nickel catalyst, the mixture is hydrogenated while shaking at atmospheric pressure for 5 hours. After absorption of 40 ml of hydrogen, the catalyst is filtered out, the filtrate is evaporated to dryness and the residue is recrystallized from methanol to give 0.47 g (94 %) of the title compound, m.p: 176-178 °C. Example 25 Preparation of 14α-hydroxy-7,8-methylenedioxyalloberban 2 g of sodium metal and 40 ml of abs. xylene are weighed in a 100 ml round-bottom flask. The xylene is heated to boiling by using an infra-red lamp and the molten sodium is dispersed by vigorously shaking the flask. The thus-dispersed sodium is stirred in the xylene by using an electro-magnetic stirrer, cooled to 0 °C, then 0.5 g of 7.8-methylenedioxy-14-oxoalloberban (1.67 mmoles) is portionwise added to the suspension. To this solution 5 ml of abs. ethanol are dropped from a dropping funnel during 2 hours, then the mixture is stirred for one additional hour and then evaporated to dryness by using a rotavapor device. The residue is shaken with 10 ml of water and 10 ml of dichloromethane, the dichloromethane phase is dried and evaporated to dryness. After recrystallizing the residue from methanol, the title compound is obtained in a yield of 0.42 g (84 %), m.p.: 214-217 °C. Example 26 Preparation of a tablet containing 10 mg of the active ingredient 10 g of 14α-hydroxy-7,8-methylenedioxy-alloberban are homogenized with 6 g of talc, 6 g of magnesium stearate, 20 g of polyvinylpyrrolidone, 90 g of maize starch and 160 g of lactose, then 1000 tablets are prepared from this mixture by compression.

Claims: Claims for the following Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL, SE Racemic or optically active compounds of general formula (I) wherein R¹ and R² represent independently from each other a hydroxyl or straight or branched C₁₋₆ alkoxy group or R¹ or R² together represent a C₁₋₃ alkylenedioxy group; R³ and R⁴ represent independently from each other a hydrogen atom or a straight or branched C₁₋₄ alkyl group (optionally substituted by a hydroxyl group) or a C₂₋₆ alkoxycarbonyl or cyano group, with the proviso that when R¹ and R² are both methoxy groups then R³ and R⁴ are not cyano, methoxycarbonyl or hydroxymethyl groups and R³ and R⁴ are not both hydrogen atoms; and R⁵ represents a hydrogen atom or a straight or branched C₁₋₆ alkyl group, a C₁₋₇ aliphatic or aromatic acyl group or C₁₋₇ alkylsulphonyl or arylsulphonyl group, and their salts. Compounds according to claim 1, being: methyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate; methyl 14β-hydroxy-7,8-methylenedioxy-alloberban-15-carboxylate; 14β-hydroxy-7,8-methylenedioxyalloberban; (+)-(1R, 12S, 14S, 17R)-14-hydroxy-7,8-methylenedioxyalloberban; (-)-(1S, 12R, 14R, 17S)-14-hydroxy-7,8-methylenedioxyalloberban; methyl 14α-hydroxy-7,8-methylenedioxy-berban-13-carboxylate; methyl 14α-hydroxy-7,8-methylenedioxy-berban-15-carboxylate; methyl-14β-hydroxy-7,8-methylenedioxy-berban-15-carboxylate; 14β-hydroxy-7,8-methylenedioxyberban; methyl 14β-hydroxy-7,8-methylenedioxy-epialloberban-13-carboxylate; 14α-hydroxy-7,8-methylenedioxy-epialloberban; 7,8-diethoxy-14β-hydroxyalloberban; 14α-hydroxy-7,8-methylenedioxyalloberban; 14β-hydroxy-13-hydroxymethyl-7,8-methylenedioxyalloberban; 14β-hydroxy-15-hydroxymethyl-7,8-methylenedioxyalloberban; 14β-hydroxy-13-hydroxymethyl-7,8-methylenedioxyberban; 7,8-dihydroxy-14β-hydroxyalloberban; 15-cyano-14β-hydroxy-7,8-methylene-dioxyalloberban; 14β-acetoxy-7,8-methylenedioxyalloberban; 14α-acetoxy-7,8-methylenedioxyalloberban; or ethyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate; A pharmaceutical composition which comprises as active ingredient(s) one or more compounds of the general formula (I) according to claim 1 or pharmaceutically acceptable salt thereof. A process for the preparation of a compound according to claim 1, which comprises reducing the oxo group of racemic or optically active compound of the general formula (II) (wherein R¹, R², R³ and R⁴ are as defined in claim 1), and then optionally alkylating or acylating a compound of formula (I) in which R⁵ is a hydrogen atom to obtain a compound in which R⁵ is as defined in claim 1 but other than hydrogen, and optionally resolving the compound of formula (I) or transforming it into a salt or liberating it from a salt. A process according to claim 4, which comprises using a metal hydride as reducing agent. A process according to claim 4, which comprises using a sulphur-containing compound as reducing agent. A process according to claim 4, which comprises carrying out the reduction by using hydrogen in the presence of a catalyst. A process according to claim 4, which comprises using Raney nickel as catalyst. A process according to claim 4, which comprises using a system consisting of an alcohol and an alkaline metal as reducing agent. A process according to claim 4, in which an optically active compound of general formula (II) is used as starting material. A process according to claim 4, in which an acyl halide is used as acylating agent. A process for the preparation of a pharmaceutical composition according to claim 3, which comprises mixing one or more compounds of formula (1) as defined in claim 1 or a pharmaceutically acceptable salt thereof with carriers and/or additives commonly used in the pharmaceutical industry and forming a pharmaceutical composition. Claims for the following Contracting States: AT A process for the preparation of a racemic or optionally active compound of general formula (I) wherein R¹ and R² represent independently from each other a hydroxyl or straight or branched C₁₋₆ alkoxy group or R¹ or R² together represent a C₁₋₃ alkylenedioxy group; R³ and R⁴ represent independently from each other a hydrogen atom or a straight or branched C₁₋₄ alkyl group (optionally substituted by a hydroxyl group) or a C₂₋₆ alkoxycarbonyl or cyano group, with the proviso that when R¹ and R² are both methoxy groups then R³ and R⁴ are not cyano, methoxycarbonyl or hydroxymethyl groups and R³ and R⁴ are not both hydrogen atoms; and R⁵ represents a hydrogen atom or a straight or branched C₁₋₆ alkyl group, a C₁₋₇ aliphatic or aromatic acyl group or C₁₋₇ alkylsulphonyl or arylsulphonyl group, or a salt thereof, which comprises reducing the oxo group of racemic or optically active compound of the general formula (II) (wherein R¹, R², R³ and R⁴ are as defined above), and then optionally alkylating or acylating a compound of formula (I) in which R⁵ is a hydrogen atom to obtain a compound in which R⁵ is as defined above but other than hydrogen, and optionally resolving the compound of formula (I) or transforming it in to a salts or liberating it from a salt. A process according to claim 1 in which the product is: methyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate; methyl 14β-hydroxy-7,8-methylenedioxy-alloberban-15-carboxylate; 14β-hydroxy-7,8-methylenedioxyalloberban; (+)-(1R, 12S, 14S, 17R)-14-hydroxy-7,8-methylenedioxyalloberban; (-)-(1S, 12R, 14R, 17S)-14-hydroxy-7,8-methylenedioxyalloberban; methyl 14α-hydroxy-7,8-methylenedioxy-berban-13-carboxylate; methyl 14α-hydroxy-7,8-methylenedioxy-berban-15-carboxylate; methyl-14β-hydroxy-7,8-methylenedioxy-berban-15-carboxylate; 14β-hydroxy-7,8-methylenedioxyberban; methyl 14β-hydroxy-7,8-methylenedioxy-epialloberban-13-carboxylate; 14α-hydroxy-7,8-methylenedioxy-epialloberban; 7,8-diethoxy-14β-hydroxyalloberban; 14α-hydroxy-7,8-methylenedioxyalloberban; 14β-hydroxy-13-hydroxymethyl-7,8-methylenedioxyalloberban; 14β-hydroxy-15-hydroxymethyl-7,8-methylenedioxyalloberban; 14β-hydroxy-13-hydroxymethyl-7,8-methylenedioxyberban; 7,8-dihydroxy-14β-hydroxyalloberban; 15-cyano-14β-hydroxy-7,8-methylene-dioxyalloberban; 14β-acetoxy-7,8-methylenedioxyalloberban; 14α-acetoxy-7,8-methylenedioxyalloberban; or ethyl 14β-hydroxy-7,8-methylenedioxyalloberban-13-carboxylate. A process according to claim 1 or claim 2, which comprises using a metal hydride as reducing agent. A process according to claim 1 or claim 2, which comprises using a sulphur-containing compound as reducing agent. A process according to claim 1 or claim 2, which comprises carrying out the reduction by using hydrogen in the presence of a catalyst. A process according to claim 5, which comprises using Raney nickel as catalyst. A process according to claim 1 or claim 2, which comprises using a system consisting of an alcohol and an alkaline metal as reducing agent. A process according to claim 1 or claim 2, in which an optically active compound of general formula (II) is used as starting material. A process according to claim 1, in which an acyl halide is used as acylating agent. A process for the preparation of a pharmaceutical composition , which comprises mixing one or more compounds of formula (1) as defined in claim 1 or a pharmaceutically acceptable salt thereof with carriers and/or additives commonly used in the pharmaceutical industry and forming a pharmaceutical composition.

Inventors: GAAL, JOZSEF, HARSING, LASZLO, KOVACS, GABOR, MARTON, JENO, SOMOGYI, GYORGY, SZABO, LAJOS, SZANTAI, CSABA, TOTH, ISTVAN, VIZI, SZILVESZTER
Applicants: CHINOIN GYOGYSZER ES VEGYESZETI TERMEKEK GYARA RT.
Application year: 1986
Main classification: C07D455
IPC codes: A61K31, C07D455, A61P25, C07D453, C07D471