1,4-DIHYDROPYRIDINE PHARMACEUTICAL DERIVATIVES AND THEIR SALTS, AND THE PREPARATION THEREOF

Abstract: 1,4-Dihydropyridine derivatives are represented by the formula wherein one of R¹ and R² is cycloalkyl (C₃ - C₆) methyl and the other is a group -(CH₂) n (CH₂) m R⁴ in which R³ is hydrogen or a C1-3 alkyl and R⁴ is hydrogen, hydroxy, carboxyl, alkoxycarbonyl carbamoyl, phenyl, substituted (e.g. halo-, methoxy or nitro-) phenyl or trialkylsilyl, or R³ and R⁴ may link to form a N-heterocycle and n and m are each 1 to 6 Preparation can be by reacting ammonia or a salt thereof with C₆ H ₃NO ₂ -C=C(COOR ⁵ )-COCH ₃ + CH ₃ .CO. CH₂ COOR ⁶ wherein R ⁵ and R ⁶ are as defined for R¹ and R² ; the product can be reacted with an acid to form a pharmaceutical salt. Other condensations can be used. Pharmaceutical preparations of the compounds are used orally to increase cerebral blood flow.

Description: The present invention relates to 1,4-dihydropyridine derivatives, salts thereof, a process for the preparation of these derivatives and salts, and pharmaceutical agents comprising at least one of the compounds and salts as an effective ingredient. It has been known that certain kind of 1,4-dihydropyridine derivatives have a powerful calcium antagonistic effect and thus can be employed as an effective ingredient for curing diseases of the blood circulation. One known example of 1,4-dihydropyridine derivatives is the dimethyl ester of 1,4-dihydro-2,6-dimethyl 4-(2-nitrophenyl)pyridine-3,5-dicarboxylic acid (known as "Nifedipine"). This compound has the pharmaceutical activities of coronary vasodilating action and blood pressure depressing action (W. Vater et al. "Arzneim.-Forsch" (Drug Res.), Vol. 22, page 1, 1972). Another known compound is 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-­ pyridine-3,5-dicarboxylic acid 3-2-(N-benzyl-N-methylamino)ethyl 5-methyl ester (known as "Nicardipine"), which has a cerebral blood flow increasing action. Among these known compounds, Nifedipine shows a powerful depressing action but as a result, has a tendency of causing a relative decrease on cerebral blood flow. On the other hand, Nicardipine having the cerebral blood flow increasing action has been developed and clinically employed as a cerebral circulation improving agent, but it can not be said that it selectively acts on the cerebral blood path to increase the blood flow only therein. EP 138505 discloses dihydropyridine derivatives such as 5-[(2-(4-benzhydryl-1-piperazinyl)ethyl]3-ethyl-2-amino-1, 4-dihydro-6-methyl-4-(3-nitrophenyl)-3, 5-pyridinedicarboxy­ late which exhibit cardiovascular effects such as long lasting coronary and cerebral vasodilation, and a process for their production. US 4237137 discloses sila-substituted 1,4-dihydropyridine derivatives such as 2,6-dimethyl-4-(3-nitrophenyl )-1,4-di­ hydropyridine-3,5-dicarboxylic acid bis-(trimethylsilyl­ methyl) ester which are said to exhibit circulation-influen­ cing effects such as a long-lasting dilation of the coronary vessels and vascular-spasmolytic effects in the cerebral region. A principal object of the present invention is to provide novel 1,4-dihydropyridine derivatives and salts thereof, each of which has an excellent Ca antagonistic effect and acts selectively on the cerebral blood path to show a powerful cerebral blood flow increasing action. Additional objects of the invention are to provide a process for the preparation of the compounds and salts as well as a pharmaceutical agent comprising at least one of the compounds or salts, as an effective ingredient, respectively. According to the invention, we provide novel 1,4-dihydropyridine derivatives represented by the general formula wherein one of R¹ and R² is a cyclo C3-6 alkylmethyl group and the other is a group of the formula in which R³ is a hydrogen atom or C1-3 alkyl group and R⁴ is a hydrogen atom, carbamoyl radical, a phenyl radical which may be substituted with a 2-chloro-, 4-chloro-, 4-bromo-­ 4-fluoro-, 4-methoxy, 4-nitro- or 2,4-dichloro group, or a trimethylsilyl, triethylsilyl or t-butyldimethylsilyl group or R³ and R⁴ may be linked to complete a 1-pyrrolidinyl, 1-piperidinyl, 2-isoindolinyl or 2-(5-chloroisoindolinyl) ring, and n and m are each an integer of 1 to 6 or a salt thereof. In the compound of Formula I, cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl and cyclohexylmethyl radicals may be listed as examples of the cycloalkylmethyl group. As examples of the alkyl group, methyl, ethyl and propyl radicals may be listed. As examples of a substituted phenyl radical, 2-chlorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-fluorophenyl, 4-methoxy­ phenyl, 4-nitrophenyl and 2, 4-dichlorophenyl radicals may be listed. As examples of a trialkylsilyl group, trimethylsilyl, triethylsilyl and t -butyldimethysilyl radicals may be listed. Preferred salts are the fumarate and hydrochlorate. According to the process of the invention, the compounds (I) and salts thereof can be prepared by reacting in the presence of ammonia or a salt thereof, a compound represented by the formula wherein R⁵ is a cycloalkylmethyl group or the group in which R³, R⁴, n and m have the meanings defined above with a compound represented by the formula wherein R⁶ is a cycloalkylmethyl group or a group of the formula in which R³, R⁴, n and m have the meanings as referred to but R⁶ is this compound (III) and R⁵ in the compound (II) do not have same meaning as the cycloalkylmethyl group or the group respectively and, if necessary, converting the resulting reaction product into the salt. A molar ratio of 1: 0.1 to 1: 1.5 is preferable for the raw material compounds II and III. The reaction can be carried out at 50 to 150°C, in the presence or absence of a solvent. As the solvent, alcohols such as methanol, ethanol, isopropanol and the like; aromatic hydrocarbons such as benzene, toluene and the like; aprotic polar solvents such as dimethylformamide and the like; ethers such as ethylether, tetrahydrofuran and the like; or water can be employed. An isolation of the desired compound from the reaction mixture can be carried out by a known procedure, such as concentration, extraction, column chromatography, recrystallization and the like. The benzylidenes (II) as one of the raw materials may be commer­ cially available or otherwise they can be synthesized by a condensation reaction between the aldehyde represented by the formula and said ester shown by Formula III, in accordance with the method as disclosed by G. Jones in "Org. Reactions" Vol. 15, page 204, 1967. The ester (III) as the other raw material may also be commercially available, or otherwise can be synthesized in accordance with the method as disclosed by Oren-Olov Lawesson et al in "Org. Syn." Vol. V, page 155, 1973. As referred to before, ammonia or a salt thereof is employed where the compounds (I) will be synthesized, and as examples for the ammonium salt, ammonium acetate, ammonium carbonate and ammonium hydrogen carbonate may be listed. The compounds of Formula I may be synthesized through various routes other than that referred to above, and thus some of these are explained below, for reference. Route A wherein R⁵ and R⁶ have the meanings as referred to. The synthetic reaction for this route can be carried out in accordance with a method similar to that as disclosed by H. H. Fox et al in "J. Org. Chem." Vol. 16, page 1259, 1951. A molar ratio of 1 : 0.8 : 0.8 to 1 : 1.5 : 1.5 is preferable for the raw materials, and in this case, the reaction conditions, solvent and operation manner are substantially the same as with the method as described before for the process according to the invention. The aldehyde of Formula IV can be commercially obtained, and as to the ester (III), please refer to the disclosure given before for the process according to the invention. The enaminoester shown by Formula V may also be obtained commercially and otherwise can be synthesized in accordance with the method as disclosed, for instance by S. A. Glickman and A. C. Cope in "J. Am. Chem. Soc." Vol. 67, page 1017, 1945. Route B wherein R⁵ and R⁶ have the meanings as defined above This synthetic route is similar to that as disclosed by A. Singer and S. M. McElvain in "Org. Syn." Vol. II, page 214, 1943. In this case, the reaction conditions, solvent and reaction operations are substantially same with those as described before for the process according to the invention. The diketoesters of Formula VI may be of those available commer­ cially, or otherwise can be synthesized in accordance with the manner as disclosed by G. Jones in "Org. Reactions" Vol. 15, page 204, 1967 and with use of the compound shown by Formula II and a catalyst of potassium fluoride, cesium fluoride or the like. Route C wherein R⁷ is cycloalkylmethyl group and R⁸ is a group of in which R³, R⁴, n and m have the meanings as referred to. This synthetic route is similar to that as disclosed by T. Shibanuma et al in "Chem. Pharm. Bull." Vol. 28, page 2809, 1980. The carboxylic acids shown by Formula VII may be of those available commercially or otherwise, and can be synthesized in accordance with the manner disclosed in the literature. In accordance with this route, the raw material (VII) is reacted firstly with thionyl chloride, phosphorus pentachloride or a chloroformic ethyl ester in the same molar amount, in the presence of a solvent such as methylene chloride, chloroform or the like halogenated hydrocarbons, benzene, toluene or the like aromatic hydrocarbon, diethyl ether, tetrahydrofuran, dimethoxyethane, dioxane or the like ether dimethyl­ formamide or the like aprotic polar solvents or a mixture thereof and then reacted with the alcohol (VIII) in the same molar amount. Otherwise, the compounds (I) may be prepared by mixing both raw materials in the same molar amount, adding thereto dicyclohexylcarbodiimido in same molar amount and then reacting the materials in a solvent in the presence of N,N-dimethylaminopyridine in a catalytic amount. Whichever of the synthetic methods is selected for this route C, an isolation of the objective compound can be attained in the manner similar to that as disclosed before for the process according to the invention. The 1,4-dihydropyridine derivatives and salts thereof according to the invention have an excellent Ca antagonistic effect and selectively act on the cerebral blood path to cause a remarkable increase in the rate of cerebral blood flow and thus these are preferable to use as an effective ingredient for cerebral circulation improved agents. On the other hand, the process according to the invention has an advantage that these useful compounds can be prepared in relatively easy manner by starting from raw material which are easy available commer­ cially or can easily be synthesized. Each of the compounds and salts according to the invention can be made with conventional additives or carriers into a tablet, capsule, granules or the like form for oral dosage. Any pharmaceutically acceptable salt can be used. A dosing amount of the compound or salt for humans depends on the particular compound to be used, selected medicine form, condition of illness, age of patient and other factors, but in the case of an adult, 1 to 100 mg/day is generaly preferable. The invention will now be further explained with reference to the following Examples, Pharmaceutical Test Examples and Prescriptional Examples. Percentages are by weight. Example 1 A mixture of 4.0 g (16 mmol) of 2-(N-benzyl-N-methyl-­ amino)ethyl acetoacetate, 4.6 g (16 mmol) of cyclopropylmethyl 2-(3-nitrobenzylidene)acetoacetate, 1.12 ml of 28% aqueous ammonia and 40 ml of ethanol was stirred at the reflux temperature for 8 hours, and then the solvent was removed under reduced pressure. The residue was chromatographed on a silica gel column to yield 3-cyclopropylmethyl 5-[2-(N-benzyl-N-methylamino)-ethyl)] 1,4-dihydro-2, 6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate (3.5 g, 42.1%) as yellow oil. Treatment of this oil with hydrochloric acid gave the corresponding salt. ′H-NMR spectrum (CDCl₃)δ ppm: 0.01-1.37 (5H, m) 2.37 (3H, s) 2.43 (3H, s) 2.66 (3H, s) 3.23-3.57 (2H, m) 3.87 (2H, d) 4.37 (2H, s) 4.33-4.70 (2H, m) 5.11 (1H, s) 7.43-8.20 (9H, m) 9.56 (1H, s) 11.70 (1H, brs) Mass spectrum (EI/DI) m/z: 519 (M⁺), 148, 134 (base peak) Elementary analysis (C₂₉H₃₃N₃O₆ · HCl · 1/2H₂O): Cal. ; H 6.24, C 61.64, N 7.43 Found ; H 6.26, C 61.76, N 7.33 Example 2 A mixture of 13.0 g (75 mmol) of 2-(N,N-dimethylamino)ethyl aceto­ acetate, 21.7 g (75 mmol) of cyclopropylmethyl 2-(3-nitrobenzylidene)acetoacetate, 6.0 ml of 28% aqueous ammonia and 70 ml of ethanol was stirred at the reflux temperature for 7 hours, and then the solvent was removed under reduced pressure. The residue was chromatographed on a silica gel column and crystallized from ethyl ether to yield 3-cyclo­ propylmethyl 5-[2-(N,N-dimethylamino)ethyl] 1,4-dihydro-2,6-dimethyl-­ 4-(3-nitrophenyl)pyridine-3,5-dicarboxylate (14.1 g, 42.3%) as yellow crystals. ′H-NMR spectrum (CDCl₃)δ ppm: 0.00-1.50 (5H, m) 2.27 (6H, s) 2.30 (6H, s) 2.57 (2H, t) 3.92 (2H, d) 4.19 (2H, t) 5.22 (1H, s) 6.30 (1H, s) 7.30-8.30 (4H, m) Mass spectrum (EI/DI) m/z: 443 (M⁺), 71 (base peak) Elementary analysis (C₂₃H₂₉N₃O₆): Cal. ; H 6.59, C 62.27, N 9.47 Found ; H 6.63, C 62.08, N 9.47 Melting point: 125 - 126°C Example 3 A mixture of 10.0 g (35 mmol) of 2-[N-(4-chlorobenzyl)-N-methylamino]ethyl acetoacetate, 10.2 g (35 mmol) of cyclopropylmethyl 2-(3-nitro­ benzylidene)acetoacetate, 2.9 ml of 28% aqueous ammonia and 50 ml of ethanol was stirred at the reflux temperature for 7 hours, and then the solvent was removed under reduced pressure. The residue was chromato­ graphed on silica gel column and crystallized from methanol to yield 3-[2-[N-(4-chlorobenzyl)-N-methylamino]ethyl] 5-cyclopropylmethyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate (11.0 g, 56.3%) as yellow crystals. ′H-NMR spectrum (CDCl₃)δ ppm: 0.00-1.33 (5H, m) 2.16 (3H, s) 2.37 (6H, s) 2.58 (2H, t) 3.43 (2H, s) 3.86 (2H, d) 4.16 (2H, t) 5.16 (1H, s) 6.13 (1H, brs) 7.20-8.30 (8H,m) Mass spectrum (EI/DI) m/z: 553 (M⁺), 181 (base peak) Elementary analysis (C₂₉H₃₂CIN₃O₆): Cal. ; H 5.82, C 62.85, N 7.58 Found ; H 5.90, C 62.52, N 7.53 Melting point: 115 - 116.5°C Example 4 A mixture of 8.8 g (30 mmol) of 2-[N-(4-methoxybenzyl)-N-methylamino]ethyl acetoacetate, 8.5 g (30 mmol) of cyclopropylmethyl 2-(3-nitro­ benzylidene)acetoacetate, 2.5 ml of 28% aqueous ammonia and 50 ml of ethanol was stirred at the reflux temperature for 7 hours, and then the solvent was removed under reduced pressure. The residue was chromato­ graphed on silica gel column to yield 3-cyclopropylmethyl 5-[2-[N-(4-­ methoxybenzyl)-N-methylamino]ethyl] 1,4-dihydro-2,6-dimethyl-4-(3-nitro­ phenyl) pyridine-3,5-dicarboxylate (7.8 g, 46.7%) as a yellow oil. Treatment of this oil with 1 equivalent of fumaric acid in ethanol gave the corresponding fumarate. ′H-NMR spectrum (DMSO-d₆)δ ppm: 0.00-1.50 (5H, m) 2.16 (3H, s) 2.34 (6H, s) 2.40-2.90 (2H, m) 3.53 (2H, s) 3.77 (3H, s) 3.85 (2H, d) 4.16 (2H, t) 5.12 (1H, s) 6.71 (2H, s) 6.93 (2H, d) 7.24 (2H, d) 7.35-8.25 (4H, m) 8.13 (1H, brs) Mass spectrum (EI/DI) m/z: 549 (M⁺), 177 (base peak) Elementary analysis (C₃₄H₃₉N₃O₁₁ ·1/2H₂O) Cal. ; H 6.12, C 60.43, N 6.22 Found ; H 6.00, C 60.48, N 6.22 Melting point: 118 - 130°C Example 5 A mixture of 15.3 g (53 mmol) of 2-(N-methyl-N-trimethylsilylmethyl­ amino)ethyl acetoacetate, 13.0 g (53 mmol) of cyclopropylmethyl 2-(3-­ nitrobenzylidene)acetoacetate, 4.4 ml of 28% aqueous ammonia and 50 ml of ethanol was stirred at the reflux temperature for 7 hours, and then the solvent was removed under reduced pressure. The residue was chromato­ graphed on silica gel column to yield 3-cyclopropylmethyl 5-[2-(N-­ methyl-N-trimethylsilylmethylamino)ethyl] 1,4-dihydro-2,6-dimethyl-4-(3-­ nitrophenyl)pyridine-3,5-dicarboxylate (7.0 g, 25.7%) as a yellow oil. Treatment of this oil with 1 equivalent of fumaric acid in ethanol gave the corresponding fumarate. ′H-NMR spectrum (DMSO-d₆)δ ppm: 0.00 (9H, s) 0.03-1.50 (5H, m) 2.13 (2H, s) 2.33 (9H, s) 2.77 (2H, t) 3.80 (2H, d) 5.05 (1H, s) 6.65 (2H, s) 7.50-8.25 (4H, m) 9.09 (1H, brs) Mass spectrum (EI/DI) m/z: 515 (M⁺), 130 (base peak) Elementrary analysis (C₃₀H₄₁N₃O₁₀Si · 1/2H₂O Cal. ; H 6.61, C 56.23, N 6.56 Found ; H 6.64, C 56.38, N 6.30 Melting point: 73 - 76°C Example 6 A mixture of 6.57 g (30.4 mmol) of 2-(N-carbamoylmethyl-N-methylamino)ethyl acetoacetate, 8.79 g (30.4 mmol) of cyclopropylmethyl 2-(3-nitro­ benzylidene)acetoacetate, 2.4 ml of 28% aqueous ammonia and 30 ml of ethanol was stirred at the reflux temperature for 7 hours, and then the solvent was removed under reduced pressure. The residue was chromato­ graphed on silica gel column and crystallized from ethylether to yield 3-[2-(N-carbamoylmethyl-N-methylamino)ethyl 5-cyclopropylmethyl 1,4-­ dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate (8.67 g, 58.7%) as yellow crystals. ′H-NMR spectrum (CDCl₃ + DMSO-d₆)δ ppm: 0.00-1.40 (5H, m) 2.31 (3H, s) 2.38 (6H, s) 2.67 (2H, t) 2.98 (2H, s) 3.87 (2H, d) 4.19 (2H, t) 5.16 (1H, s) 7.03 (2H, brs) 7.38-8.30 (4H, m) 8.92 (1H, brs) Mass spectrum (EI/DI) m/z: 486 (M⁺), 469 (base peak) Elementary analysis (C₂₄H₃₀N₄O₇) Cal. ; H 6.21, C 59.22, N 11.51 Found ; H 6.52, C 59.07, N 11.46 Melting point: 176 - 178°C Pharmaceutical Test Example 1 (Ca Antagonistic Effect) Ca antagonistic effect was examined on a spiral strip of New Zealand white rabbit aorta by the Magnus method. The response of preparations was recorded isometrically on a recorder. Contraction induced Ca (25 mM) after K depolarization in Ca free solution was compared between those obtained when a test or control compound was added and when no compound was added. The data given in following Table 1 are presented as inhibitory % calculated based on concentration obtained when neither the test compound nor control compound was added. Pharmaceutical Test Example 2 (Negative inotropic effect) New Zealand white rabbit was killed by bleeding from the carotid artery and the left atrium was quickly removed. The left atrium was suspended in an organ bath where Tyrode's solution was kept at 37°C and bubbled with a gas mixture of 95% 0₂-5% CO₂, and the atrium was given electric stimulation (1Hz, 2 msec, 10V). The response of preparations was recorded isometrically on a recorder. The initial tension applied to the preparation was adjusted to 1 g. Results are shown in following Table 2. Pharmaceutical Test Example 3 (Effect of compounds on vertebral and femoral arterial blood flow) An experiment was carried out by anesthetizing adult male and female mongrel dogs (weight; 10-20 kg) with Nembutal (30 mg/kg i.p.) and artifically respiring (20 ml/kg/stroke, 20 strokes/min) the animals. Right vertebral and femoral arteries were exposed and each probe of electro-magnetic flow meter was fitted therein the measure vertebral and femoral arterial blood flow. A cannula was inserted into the descending aorta from left femoral artery in order to measure blood pressure by a pressure transducer connected to an amplifier. Each test compound dissolved in 50% ethanol solution (1 µg/kg, i.v.) was administered from the cannula into the cephalic vein. Results of change in blood flow and blood pressure are given in the following Table 3. Pharmaceutical Test Example 4 (Acute toxicity) Male and female dd mice were used (weight: 18-20 g). Each of the compounds to be tested was dissolved in 1% Nikkol and administered directly into stomach by forced oral administration and with use of a metal sonde. LD₅₀ of the compounds were calculated by the Litchfield-Wilcoxon method, after observation for 72 hours. Results are shown in the following Table 4. Prescriptional Example 1 (Tablets) Following components were mixed to prepare tablets in a conventional manner. Prescriptional Example 2 (Capsules) Following components were mixed and charged into gelatin capsules in a conventional manner to prepare capsuled medicine. Prescriptional Example 3 (Tablets) Following components were mixed to prepare tablets in a conventional manner. Prescriptional Example 4 (Capsules) Following components were mixed and charged into gelatin capsules in a conventional manner to prepare capsuled medicine.

Claims: 1. A 1,4-dihydropyridine derivative represented by the formula wherein one of R¹ and R² is a cyclo C3-6 alkylmethyl group and the other is a group of the formula in which R³ is a hydrogen atom or C1-3 alkyl group and R⁴ is a hydrogen atom, carbamoyl radical, a phenyl radi­ cal which may be substituted with a 2-chloro-, 4-chloro-, 4-bromo-, 4-fluoro-, 4-methoxy, 4-nitro- or 2,4-dichloro group, or a trimethylsilyl, triethylsilyl or t-butyldimethylsilyl group or R³ and R⁴ may be linked to complete a 1-pyrrolidinyl, 1-piperidinyl, 2-isoindolinyl or 2-(5-chloroisoindolinyl) ring, and n and m are each an integer of 1 to 6, or a salt thereof. 2. A compound as claimed in Claim 1 or 2, wherein R⁴ is a 2-chlorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-fluorophenyl, 4-methoxyphenyl, 4-nitrophenyl, 2,4-dichloro­ phenyl, trimethylsilyl, triethylsilyl or t-butyldiemthylsilyl radical. 3. A compound as claimed in Claim 1 or 2 wherein said salt is a fumarate or hydrochlorate. 4. A compound as claimed in Claim 1, wherein said derivative is 3-cyclopropylmethyl 5-[2-(N-benzyl-N-methylamino)ethyl]-1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate, 3-cyclopropylmethyl 5-[2-(N,N-dimethylamino)ethyl]­ 1, 4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate, 3-[2-(N-(4-chlorobenzyl)-N-methylamino]ethyl] 5-cyclopropyl-­ methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate, 3-cyclopropylmethyl 5-[2-(N-methoxybenzyl)-N-methylamino] ethyl] 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate, 3-cyclopropylmethyl 5-[2-(N-methyl-N-trimethylsilyl-methyl-­ amino)ethyl] 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate or 3-[2-(N-carbamoylmethyl-N-methylamino)ethyl 5-cyclopropyl-­ methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)pyridine-3,5-dicarboxylate. 5. A process for the preparation of a 1,4-dihydro­ pyridine derivative or a salt thereof as claimed in Claim 1, which comprises reacting in the presence of ammonia or a salt thereof a compound represented by the formula wherein R⁵ is a cyclopropylmethyl, cyclobutylmethyl, cyclo­ pentylmethyl or cyclohexylmethyl radical or a group in which R³, R⁴ n and m have the meanings defined in Claim 1, with a compound represented by the formula wherein R⁶ is a cyclopropylmethyl, cyclobutylmethyl, cyclo­ pentylmethyl or cyclohexylmethyl radical or a group in which R³, R⁴, n and m have the meanings as defined in Claim 1, and if necessary, converting the resulting reaction product into the salt. 6. A process as claimed in Claim 5, wherein the molar ratio of compounds II and III is 1 : 0.8 to 1 : 1.5. 7. A pharmaceutical agent which comprises as an effective ingredient at least one 1,4-dihydropyridine derivative or a salt thereof as claimed in any of Claims 1 to 4. 8. A 1,4-Dihydropyridine derivative or salt thereof as claimed in any of Claims 1 to 4, or a pharmaceutical agent as claimed in Claim 7 for the manufacture of a medicament for preventing or curing circulatory diseases.

Inventors: FUKUSHIMA, MASATO, HAMASE, KENJI, KONDO, YASUAKI, KURONO, MASAYASU, SAWAI, KIICHI, SUZUKI, TOMOO, SUZUKI, TSUNEMASA, USUI, TOSHINAO
Applicants: SANWA KAGAKU KENKYUSHO CO., LTD.
Application year: 1986
Main classification: C07D211
IPC codes: C07D211