PREPARATION OF N-SUBSTITUTED-3-CYANO AZETIDINE DERIVATIVES

Abstract: N-substituted-3-cyano azetidine derivatives are prepared by reacting the corresponding azetidine-3-ol with an alkylsul­ phonyl halide, followed by reaction with an alkali metal cyanide in the presence of lipophilic phase transfer catalyst.

Description: PREPARATION OF N-SUBSTITUTED-3-CYANO AZETIDINE DERIVATIVES This invention relates to a process for the preparation of N-substituted-3-cyano azetidine derivatives, which are intermediates for the preparation of biologically active compounds. It is known to prepare cyano compounds by nucleophilic displacement reaction between an alkali netal cyanide and a compound RX, wherein X is a suitable leaving group such as a halide or sulpho@ate. Such a reaction usually inv@lves both aqueous and organic liquid phases, resulting in a relatively slow rate of reaction. An established technique to accelerate such biphase reactions is to use a phase-transfer catalyst, such as a quaternary ammonium salt. However, in sari reactions the quaternary ammonium cation pairs with the leaving group anion to form a product which is very soluble in the organic phase, which necessitates the use of the quaternary ammonium phase transfer agent in considerably greater than purely catalytic arrounts. This applies particularly with the displacement of tosyloxy or mesyloxy groups, since quaternary ammonium tosylates and mesylates are extremely soluble in organic solvents, and almost equimlar amounts of "catalyst" are required; see, for example, J. Chem. Soc., 954 (1962); Tetrahedron, 27, 4057 (1971); Synthesis, 430 (1975). The mesyloxy group is often a commercially attractive choice of leaving group, but the need for such large proportions of phase transfer agent clearly iirposes a serious economic handicap, and it would therefore be a commercially useful technical advance if this handicap could be overccm. The Applicants have now discovered that a selected group of phase transfer agents are effective at merely catalytic proportions in promoting the mesylate/cyanide displacement reaction. Accordingly, the present invention provides a process for the preparation of N- substituted- 3-cyano-azetidine derivatives of the formula I: EMI2.1 in which RI represents an arylmethyl group and R2 represents a hydrogen atan or an alkyl, aryl or aralkyl group in which the alkyl moiety contains up to 8 carbon atoms, by reacting a N-substituted azetidine-3-ol of forirula II: EMI2.2 in a water-imniscible organic solvent with an alkylsulphonyl halide, followed by reaction of the resulting organic solution of the 3-alkylsulphonyl azetidine product with an aqueous solution of an alkali metal cyanide in the presence of a catalytic proportion of a lipophilic phase transfer catalyst. Preferably the group R1 represents a monoarylmethyl group, suitably benzyl; R2 represents a hydrogen atan; and the alkylsulphonyl halide is a chloride, in particular methylsulphonyl (mesyl) chloride. The organic solvent used in the first, sulphonation, stage should be water-immiscible in order to enable the subsequent cyanation to be carried out in a two phase system, thereby facilitating the removal of excess cyanide ions and inorganic salts via the discarded aqueous phase. Most conventional water-immiscible organic solvents may be used, though it has been found that yields are less good with aliphatic chlorohydrocarbons. Thus suitable organic solvents include esters, such as ethyl acetate, and optionally halogenate@ aramaic hydrocarbons, such as toluene and chlorobenzene. The alkali metal cyanide is, for reasons of commercial economics, usually sodium cyanide and, as already stated, is used in an aqueous solution and the resulting two phase reaction system is promoted by the presence of a phase transfer catalyst. applicants have found that some widely used phase transfer catalysts are not effective in promoting the desired cyanation, and that this lack of efficacy appears to be found in those phase-transfer catalysts which are lipophobic, such as hexadecyltributylphosphonium bromide, tetrabutylammonium branide, and crown ethers such as 18-crown-6. Accordingly, as specified above, the phase transfer catalyst should be lipophilic, i.e. organic soluble/water insoluble. Compounds suitable as phase transfer catalysts are tetraalkyl ammonium halides in which the alkyl groups contain a total of at least 20 carbon atoms, in particular such tetraalkyl ammonium halides which contain at least 1 ethyl group and at least two alkyl groups containing at least 8 carbon atoms. Many of the carrrercially available quaternary ammonium salts are derived fran natural products e.g. tallow, and therefore contain alkyl groups of varying chain length; the foregoing figures refer, in such cases, to the mean chain length of the different alkyl groups presents. The proportion of phase transfer catalyst may be within the levels normally used in the catalysis of such two phase systems, being significantly less than equimolar and normally not more than 20 rrol%. Taking both practical and economic factors into account, preferred proportions are frown 1 to 20, preferably frown 2 to 10, irol %. The first reaction step between the alcohol and the alkylsulphonyl halide is exothermic, and the 3-alkylsulphonyl azetidine formed is liable to partial decomposition at elevated temperatures. It is therefore desirable to carry out this first step with cooling to prevent the reaction temperature exceeding roan temperature (250C), preferably cooling sufficiently to maintain a reaction temperature belay 250C but above OOC, e.g. between 50C and 150C. The second reaction step is suitably carried out at elevated teeeerature, conveniently by reflexing the reaction mixture. The starting azetidin-3-ol of formula II may be prepared by appropriate adaptations of known synthetic procedures. A preferred synthesis route to that product is described and claieed in Applicants' copending UK Patent Application No. 8412814 (K 1946), namely by cyclizing a solution in triethylamine of an aminoalcohol of formula III: EMI4.1 (wherein R1 and R2 are as defined above and Hal denotes a halogen atan) As mentioned above, the N-substituted-3-cyano-azetidine derivatives of formula I are useful intermediates. Thus, they rtay be converted by known procedures, for exwple, via the corresponding 3-carboxylic acid derivativve, to azetidine-3-carboxylic acid derivatives, which exhibit plant growth regulant properties, especially the property of rendering sterile the stale parts of plants. The invention therefore includes the use of N-substituted-3-cyano-azetidine derivatives prepared by the process of the invention, as intermediates for the preparation of azetidin-3-carboxylic acid derivatives. The invention is illustrated in the following Examples. Example 1 Preparation of N-benzyl-3-cyano azetidine a) N-benzylazetidin-3-ol (4.75kg), triethylamine (3.11kg) and toluene (33 1) were charged into a reactor and stirred until the alcohol had dissolved. Methylsulphonyl chloride (3.51kg) was added over 2 hours and the reaction mixture stirred for 2 hours with cooling to 7-120C. The product was then suuuitted to phase separation, a water wash was applied, and the resulting toluene solution of N-benzyl-3-mesyloxy azetidine used directly for the subsequent cyanation step. b) Further toluene (2 1) was added to the aqueous phase resulting fran a), and charged to a reactor together with water (10 1) sodium cyanide (2.83kg) and the phase transfer catalyst "Adogen" 464 (1.29kg dissolved in 2.8 1 toluene). "Adogen" 464 is a proprietary trialkylmethyl ammonium chloride in which each alkyl group (except the methyl group) contains 8, 9 or 10 carbon atans. This mixture was refluxed (870C) with vigorous agitation for 1 hour, then cooled to room temperature (23 C), and the phases separated. The organic phase was then washed with water and the solvent removed by evaporation. Distillation yielded 3.77kg of the desired product, purity about 87%, a yield of 68% based on the starting azetidin-ol. Examples 2-11 Following procedures similar to those described in Example 1, but on a smaller scale, the suitability of alternative solvents and phase transfer agents was evaluated with the results set out in the Table below. Except where otherwise stated, the concentrations of alcohol in the solvent was 1M, the nesylation temperature around 10 0C, the amount of PTC used was 10 rrol% and the cyanide used at a 6 molar concentration in an amount to provide 2 equivalents. The phase transfer catalysts (PTC) used in Examples 8-10 are not lipophilic; these examples are therefore not illustrative of the invention but are included for the purposes of comparison with the yields obtained in the other Examples using the lipophilic PTCs according to this invention. Table EMI6.1 Example <SEP> Solvent <SEP> Phase <SEP> Transfer <SEP> Catalyst <SEP> Cyanation <SEP> conditions <SEP> Yield <tb> 2 <SEP> Ethyl <SEP> Acetate <SEP> "Adogen" <SEP> 464 <SEP> 3/4hr <SEP> reflux <SEP> 51 <tb> 3 <SEP> Chlorobenzene <SEP> "Adogen" <SEP> 464 <SEP> 30 <SEP> min <SEP> reflux <SEP> 62 <tb> 4 <SEP> Toluene <SEP> (C7H15)4NBr <SEP> 1hr <SEP> reflux <SEP> 64 <tb> 5 <SEP> Toluene <SEP> "Arquad" <SEP> 2C-75/10 <SEP> 1hr <SEP> reflux <SEP> 72 <tb> 6 <SEP> Toluene <SEP> "Arquad" <SEP> 2HT-75/10 <SEP> 1hr <SEP> refulx <SEP> 68 <tb> 7 <SEP> Toluene <SEP> (C18H37)2(CH3)2(CH)2NBr <SEP> 5hr <SEP> reflux <SEP> 76 <tb> 8 <SEP> Toluene <SEP> (C4H9)3C16H33PBr <SEP> (5%) <SEP> 4hr <SEP> 80 C <SEP> 33 <tb> 9 <SEP> Toluene <SEP> 18-crown-6 <SEP> (3%) <SEP> 18hr <SEP> 0 <tb> 10 <SEP> Toluene <SEP> (C4H9)4NBr <SEP> 1hr <SEP> reflux <SEP> 45 <tb> "Arquad" t-75/10 and 2HT-75/10 are dimethyl dialkyl ammonium chlorides in which the alkyl groups are derived, respectively, fran coconut oil (average carbon chain length C12 50%; C14 20%) and hydrogenated tallow (average carbon chain length C16 31%, C18 64%. The results shown in the above Table denonstrate clearly that use of a water-soluble PTC (Examples 8-10) results in unacceptably low yields, whereas the lipophilic PTC of the present invention, especially using toluene as solvent, enables good yields to be achieved. Example 11 Use of N-benzvl-3-cyanoazetidine to prepare azetidin-3carboxylic acid a) 1-Benzyl-3-cyanoazetidine (0.5g) in saturated barium hydroxide solution (10ml) was heated under reflux for 30 hours. The reaction mixture was cooled, saturated with gaseous carbon dioxide and filtered. The solvent was removed fran the filtrate under reduced pressure to give 1-benzylazetidine-3-carboxylic acid in 80% yield. b) l-Benzylazetidine-3-carboxylic acid (0.5g, prepared as in a)) in methanol (15ml) was hydrogenated in the presence of a 5% palladi@@ cn carbor catalyst at - temperature. The catalyst was filtered off and the solvent removed fran the filtrate under reduced pressure to give azetidin-3-carboxylic acid in 90% yield.

Claims: CLAIMS 1. A process for the preparation of N-substituted- 3- cyano-azetidine derivatives of the formula I: EMI8.1 in which RI represents an arylmethyl group and # represents a hydrogen atan or an alkyl, aryl or aralkyl group in which the alkyl piety contains up to 8 carbon atoms, by reacting a N-substituted azetidin-3-ol derivative of formula II: EMI8.2 in a water-iTrmiscible organic solvent with an alkylsulphonyl halide, followed by reaction of the resulting organic solution of the 3-alkylsulphonyl azetidine product with an aqueous solution of an alkali metal cyanide in the presence of a catalytic proportion of a lipophilic phase transfer catalyst. 2. Process as claind in claim 1 wherein R1 represents a benzyl group and R2 represents a hydrogen atan. 3. Process as claitred in claims 1 or 2 wherein the alkylsulphonyl halide is methylsulphonyl chloride. 4. Process as claimed in claims 1, 2 or 3 wherein the organic solvent is toluene or chlorobenzene. 5. Process as claimed in any one of claims 1-4 wherein the phase transfer catalyst is a tetraalkyl ammonium halide in which the alkyl groups contain a total of at least 20 carbon atoms. 6. Process as clained in claim 5 wherein the tetraalkyl ammonium halide contains at least 2 alkyl groups containing at least 8 carbon atoms and at least one methyl group. 7. Process as claimed in any one of the preceding claims wherein the phase transfer catalyst is present in an arrcunt of fran 1 to 20 mol%, based on the starting alcohol. 8. Process as claimed in any one of the preceding claims wherein the reaction between the alcohol of formula II and the alkylsulphonyl halide is carried out with cooling to maintain the reaction temperature belw 250C, and the subsequent reaction with alkali metal cyanide is carried out at elevated tanperature. 9. N-substituted-3-cyano azetidine derivatives of formula I, when prepared by a process as claimed in any one of claims 1-8. 10. The use of N-substituted-3-cyano azetidine derivatives of formula I prepared by a process as claleed in any one of claims 1-8, as intermediates for the preparation of azetidine-3-carboxylic acid derivatives.

Inventors: BRINER, PAUL HOWARD, MASON, RONALD FRANK
Applicants: SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.
Application year: 1985
Main classification: C07D205
IPC codes: C07D205