IMPROVED METHOD FOR CULTURING MICROORGANISMS

Abstract: The culturing of microorganisms in a nutrient medium is improved by adding a zeolite to the nutrient medium. The zeolites can be synthetic or natural.

Description: IMPROVED -MErHOD FOR CULTURING MICROORGANISMS -1 The present invention relates to the culturing of microorganisms. Zeolites are hydrated aluminosilicate minerals, are well known ion-exchange agents and are able to differentially absorb molecules in their lattice structures. Zeolites and be natural or synthetic and are usually represented by the empirical formula: M¯/nO.A1203.xSiO2.yH2) wherein n is the cation valence, x is greater than or equal to 2 and y is the function of the porosity of the framework. Zeolites are used in industry particularly as catalysts and molecular sieves. For a general description of zeolites for industrial uses, see Dwyer et al, Zeolites - Asl Introduction, Chemistry and Industry. April, 1984. U.S. Patent 4,370,234 discloses a method and apparatus for treating waste water containing ammonia by passing the waste water over a bed of ion-exchange particles capable of exchanging ammonium and having a growth of nitrifying bacteria thereon. The waste water is preferably first oxygenated and then passed through a biological filter, solids filter and an organic filter before reaching the ion-exchange particles. Suitable ionexchange particles disclosed in this patent include zeolites, i.e., clinoptilolite. Horsch, CM. and Holway, J.E., International Committee on Natural Zeolites, pages 229-237, (1983), dis close clinoptilolite useful as a biofilter medium in a water-reconditioning system used in a fish hatchery. Clinoptilolite was picked as the nitrogen (NH4+) from water. The object of the present invention is to provide an improved method for the culturing of microorganisms. The present invention provides a method of culturing microorganisms in a nutrient medium, the improvement which comprises adding to the nutrient medium an amount of a zeolite effective to enhance the growth of liicroorganisms. In the drawings: Figure 1 is a cross sectional view of a slow sand filter (SSF): Figure 2 is a graph of the weekly average high and low air temperatures; Figure 3 is a g aph of effluent nitrate concentrations; Figure 4 is a graph of effluent coliforms per 100 ml sampl#; Figure 5 is a graph of volatile solids of schmutzdecke of zeolite SSF: Figure 6 is a graph of volatile solids of control SSF; Figure 7 is a graFh of filtration rates during winter operation of SSF; Figure 8 is a graph of filtration rates during spring runoff; Figure 9 is a bar graph showing various media configurations; Figure 10 is a graph of head loss at 15 cm. depth in columns; Figure 11 is a graph of head loss at 105 cm depth in columns; and Figure 12 is a graph of nitrate concentrations. In practicing the present invention, a zeolite is added to the nutrient medium where microorganisms are being culture. The zeolite is added to the nutrient medium in an amount effective to enhance the growth of the microorganisms growing therein. Zeolite can be added in amounts ranging from 0.1 to about 200 or more percent by volume of the nutrient medium. The exact amount of zeolite to be added will vary based on a variety of factors, such as, the specific microorganisms ' be cultured, the specific nature of the nutrient medium, the specific product(s) desired if the microorganisms are being cultured for the production of a compound(s) and the specific gas exchange conditions present in the culture. The optimal amount of zeolite to be added in any given culture can be readily determined by one skilled in the art by performing routine rate determination experiments, i.e. ,- dose titration determination. One skilled in the art will readily appreciate the wide applicibility of the present invention to various culture conditions, such as, for example, fermentation broth culturing, fermentation slurry broth culturing, the fermentation of bacteria in the rumen of a ruminant animal and slow sand filters which are actually biological filters in a sense that microoranisms are cultured in the schmutzdecke ani throughout the filter media itself wherein the microorganisms cultured therein contribute to the filtration process. See "Slow Sand Filtration" by L. Huisman and W. E. Wood, published by the World Health Organization, 1974. Zeolites employed in the present invention can be synthetic or natural zeolites or mixtures thereof. Preferred zeolites include clinoptilolite, phillipsite and mordenite. Clinoptilolite is especially preferred. In one embodiment of the present invention, a zeolite and sand are employed in preparing a sand filter medium. The zeolite is employed in an amour effective to increase the efficiency of the sand filter medium. The balance of the new sand filter mediua will be a silica sand and/or any other ingredients present in conventional sand filter media. The zeolite is mixed with the sand to form a uniform dispersion of zeolite in the sand, but preferably the zeolite is added on top of the sand to form a first water-contacting zone comprising up to about one-half of the total depth of the slow sand filter medium. The zeolite may comprise more than one half of the total depth of the sand filter medium, but is uneconomical to do so. The minimum effective amount of zeolite added as a first water-contacting zone is that amount of zeolite needed to form a thin layer on top of the sand sufficient to cover the sand. Zeolites employed in the practice of the pres ent invention can be any synthetic or natural zeolite. Mixtures of different zeolites are also employed. The exact nature of the zeolite employed is not critical to the practice of the present invention. When clinoptilolite is employed as the zeolite it will advantageously have a cationic exchange capacity greater than or equal to about 180 and a dry attrition resistance of less than 15% by ASTM standards for best results. Synthetic zeolites contemplated by the present include: include: the A series zeolites, such as (R.T.M.) LINDE/zeolite 4-A; the X series zeolites, such as, tINDE/zeo- lite 13-X; the Y series zeolites, and the pentasils, such as ZSM-5 and ZSM-ll. Natural zeolites employed in the practice of the present invention include: the analcime group of zeolites, such as, analcime and wairakite; the natrolite group of zeolites, such as natrolite and thomsonite; the heulandite group of zeolites, such as, heulandite and clinoptilolite; the phillipsite group of zeolites, sucn as, phillipisite and zeolite Na-P; the mordenite group of zeolites, such as, mordenite and ferrierite; the chabazite group of zeolites, such as, chabazite, erionite and zeolite L; and the laumontite group of zeolites, such as, laumontite. Preferred natural zeolites include clinoptilolite, phillipsite and mordenite with clinoptilolite being especially preferred. The particle size of the zeolites is not critical to the practice of the present invention Usually, however, the zeolite particles will cange in size from about 80 mesh up to about 6 mesh. Zeolite materials having a particle size of about 40 mesh and a tortuous or intricate surface morphologies are preferred. The sand used in the practice of the present invention with respect to slow sand filters is any sand employed in conventienal sand filters, such as, for example, silica sand, construction sand or builders sand. In the absence of suitable sand source, other granular substates such as crushed coral or burnt rice husk can b# substituted for the sand. As the amounts of sand required are large, it is common to employ ungraded material as excavated from natural deposits. In slow sand filtration, fine sand is usually employed and the designed rate of downward flow of water under treatment normally lies between about 0.1 and about 0.4 meters per hour. In one embodiment of the present inventioll, a slow sand filter is prepared by adding an effective filter enhancing amount of a zeolite onto the top of fine graded sand. The zeolite layer creates a first water-contacting zone comprising up to about one-half of the total depth of the slow sand filter medium. Preferably, the zeolite is added in an amount to create about 5.2 to 45. 7c. a first-water contacting zone which us roma bout sixo about eighteen inches deep. The sand, lying urlerneath the zeolite, creates a second water contacting zone. In operation raw water is admitted to the space above the zeolite and flows downward under the action of gravity. Purification takes place during the downward passage of the water through the first water contacting zone, i.e., the zeolite zone which includes a schmutzdecke resting directly on top of the zeolite, and then through the second water contacting zone, i.e., the sand zone. As the water exits the second contacting zone an underdrain collects the purified water and directs it to a storage facility. The under drain is typically gravel or porous concrete which acts also as a support for the sand filter medium The schmutzdecke which grows on the present slow sand filter media is more prolific and enhances the filtering capacity and efficiency of the slow sand filter system. The zeolite enhances the formation of schmutzdecke which does not penetrate the zeolite or zeolitetsand structure thereby enhan I ng the f 11 t e r capacity without blocking the physical structure of the filter media. In a preferred embodiment of the present invetio, clinoptilolite is used is the tirst water-contacting zone. The clinoptilolite is added on top of fine ungraded sand to form a about 14.74 to about 45.7 m. layer from/about 6 to about 18 inches deep. The sand is from about 0.60 to about 3.04 m. /about 2 to about 10 feet deep. The zeolite/sand slow sand filter medium is supported by an under drain consisting of gravel. The under drain collects the water and directs it to a storage tank. Water is admitted t the space above the zeolite and it flows downward under the action of gravity. Purification takes place during this downward passage first through the zeolite zone, i.e., the schmutzdecke and zeolite, and then through the sand zone. The downward flow of water under treatment is usually from about 0.1 to about 0.4 meters per hour. The exact flow rate can be set by adjusting the height of water on top of the zeolite zone or by adjustment of an effluent drain valve. In typical operations, the raw water entering the slow sand filter lies for several hours in the space immediately above the zeolite. The depth of water on top of the zeolite is about 0.60 to about 2.44 m. usually from about 2 to about 8 feet. When the slow sand filter 4S initially put into use, a ripening period is required in order for the schmutzdecke to form on top of the zeolite zone. The ripening period will vary and is usually complete in from about one week to about four weeks. The schmutzdecke consists of thread-like algae and numerous other forms of life. including plankton, diatoms, protozoa, rot i fe rs and bacteria. As the schmutzdecke, or filter skin, forms with the ripening of the filter, it contributes to the screening or filter efriciency of the slowsand filter system. The schmutzdecke forms the uppermost pcrtion of the first water-contacting zone and rests on top of the zeolite, i.e., clinoptilolite. The raw water which is introduced into the filter system can be any untreated surface water, i.e., river water, that is acceptable for slow sand filtration. It is preferred that the raw water hjve a turbidity of less than 20 ntu. This is rea:ily accomplished by directing the raw water into a sedimentation tank prior to addition into the sand filter system. If the raw water is supersaturated with air, the air should be desorbed prior to adding the raw water into the sand filter system. The use of supersaturated ra water will enhance head loss development which would be detrimental to the overall efficiency of the sand fil;er. Figure 1 shows a cross sectional view of a slow sand filter cell in a field-scale pilot plant facility. At the bottom of the cell is a filter medium support 11 which contains a perforated polyvinylchloride (PVC) pipe under drainage system 12. Alternatisely, the under drainage system can be a false floor of porous concrete (not shown) or a system of porous or unjointed pipes. The PVC pipe 12 is surrounded and covered with graded gravel 13 to support the sand bed 14 and prevent fine grains being carried into the drainage system. Directly above the filter medium support 11 is the fine ungraded sand 14 which about 0.60 to about 3.04 m. can be from/about 2 to about 10 rectt deep. Directly above the sand layer is the zeolite 16 layer which can be up to about half the total depth of the total sand filter medium or more. Raw water or supernatant i8 is introduced into the sand filter cell by raw water inlet 17. The float valve 19 regulates the level ofth.e supernatant 18 above the sand filter medium 20. The walls 21 of the sand filter cell are made of reinforced plastic, steel, stone, brick or concrete, according to which is most easily obtaInable at the site of the cell. A tempera'dre recorder 22 is conveniently adapted to the cell to record the water temperature of the supernatant 18. Baffles 23 are installed around the perimeter of the cell in the sand 14 zone to prevent short circuiting of water which would contaminate the effluent. The supernatant 18 provides a constant head of water above the filter medium 20 providi-ng a pressure that carries the water through the filter. As the raw water enters the cell it passes first through the schmutzdecke, (not shown) which forms and rests on top of the zeolite, and the zeolite zone or the first water-contacting zone and then passes into the sand or second water-contacting zone. When the water exits the sand or second vater-contacting zone, it is in a purified state atd passes downward through the filter medium support 11 into the perforated PVC pipe under drain 12. The purified effluent then passes through th-e PVC pipe 12 out a discharge port 24. As the water is discharged, it is directed through a valve (not shown) to control the rate of flow and then directed In to an outlet weir (not shown) which maintains a minimum water depth of the supernatant and aerates the outgoing water to some extent From the outlet welr the water is directed into a clear water reservoir (not shown) for distribution for commercial or residential use. The following examples illustrate the practice of the present invention, but should not be construed as limiting its scope. EXAMPLE 1: FIELD SCALE SLOW SAND FILTER FACILITY A field-scale slow sand filter (SSF) facility was constructed on the north bank of the Logan River in Utah. The facility consisted of a preliminary settling tank and two SSF (similar to the one shown in Figure 1 and desrribed hereinbefore) each three meters square, with pumps, piping, gauges and valves sufficient to main ain and regulate the flow of water through each of the filters independently. One SSF served as a control filter and the other SSF was amended with clinoptilolite. Both SSF were operable in September and were maintained through June. The control SSF of the facility contained ce meter of unsieved construction sand -(10S passing a 0.18 millimeter screen and 602 passing a 0.75 millimeter screen, i.e., D-10 = 0.18 millimeters and uniformity coefficient (or D-1O/D-6O) = 4.2 on a gravel filter drain. On March 29, after seven months of operation, a 15 centimeter layer of qu rtz sand (-0.6 millimeter diameter) was pl on t on the s u r f a c ( of the control SSF after its surface had been scraped. The zeolite SSF contained 80 centimeters of construction sand identical to that in the control SSF covered by a 20 entimeter layer of clinoptilolite graded to a consistency of 0.7 to 1.7 millimeter diameter. When freezing was not a problem, raw water was pumped from the Logan River into the preliminary settling tank and flowed by gravity into both filters, where the water level was controlled by flr.J valves. When freezing was a problem, raw water was pumped directly into the filter tanks and flow rates were controlled manually. Water flowed by gravity through the filters and into a V-notched weir box, where samples were taken for water quality analysis. Temnerature Recordings Strip chart thermograpns were installed at the facility and were to provide a continuous reword of air and water temperatures. A Weather-Hawk seven day remote sensing thermograph was used for continuous monitoring of the effluent water temperatures. Air temperatures were monitored using a spring-loaded thermograph mounted in a box under the railing on the side of the filters. A gat of 30 centimeters was left between the box and the side of the tank to minimize interference from the temperature of the water in the filter. Recordings were taken on weekly strip charts beginning in September and continuing until April. The raw water temperature at the facility was 100C on the first of October and decreased with minor fluctuations to a 30C an the first of December. Diurnal variations were generally on the order of + 10C. The water temperature fluctuated between 1.5 and 3.50C until the first of April, when it began to rise. By the first of May it was approximately 80C and remained at about that level throughout the remainder of .he spring r uno f f The air high and low temperatures, based on weekly averages are shown in Figure 2. The lowest air temperature measured during the winter was -290C on the 17th of January. From about the first of December to about the first of March there was always a layer of ice on the surface of the supernatant in the filters, but they continued to function. Ammoniu Reduction Ammonium chloride was added to the supernatants of both SSF of the facility on the 7th of June to determine ammonium removal efficiencies for each SSF. A solution containing 220 grams of ammonium chloride and 20 liters of water was added to each SSF of the facility, resulting in the supernatant solution containing approximately 5 milligrams per liter of ammonium nitrogen. A control solution of 5 milligrams per liter of ammonium nitrogen was also kept at 120C and sampled initially and at 24 hours for ammonium. This was done to determine whether d sorption to the atmosphere might cause significant reductions in the ammonium concentrations. Effluent samples from both cells were taken initially, and at 9, 4, 6. 9, 13, 24, 48 and 72 hours. The effluent samples were analyzed for ammonium nitrogen and nitrate nitrogen content. in influent sample was also taken before the addition of the ammonium chloride to allow a determination of background levels of ammonium and nitrate. No effluent sample from either SSF of the facility contained levels of ammonium nitrogen above 10 micrograms er liter(ug/l) which is the detection limit for the low levei indophenol ammonia test. The background level of ammonium nitrogen in the influent water was determined to be 100 ug/l. The ammonium nitrogen level in the Logan River was given as 11 micrograms per liter by McC#nnell, L. K., Master's Thesis, Utah State University, Logan, Utah, 1984. Nitrate results, see Figure 3, indicate that the nitrate nitrogen concentration leaving the zeolite cell was essentially constant at 0.5 milligrams per liter (mg/l). The concentration of nitrate nitrogen leaving the control SSF rose from a 24-hour value of 0.26 mg/l to a 72 hour value of 1.2 mg/I. At this time th: flow rates through the SSF were approximately 1.3 centimetere per hour (cm/hr) for the zeolite SSF and 3 centimeters per hour for the control SSF. The difference in the flow was due to the loss of filter capacity of the control. Coliform Removal The two SSF were evaluated for their effectiveness in removing coliform bacteria on two occasions. In both cases, the supernatant of each SSF was spiked with 20 liters of raw sewage and the effluent levels of coliform bacteria were monitored for several days. The first test began on March 19 when the supernatant water temperature was about 30C The supernatant water contaminated with sewage contained approximately 2500 coliforms per 100 ml sample. Samples were taken at 0, 2, 6, 10, 24 and 48 hours after addition of sewage and also at one week. Sample sizes ranged from 180 ml total sample volume initially, to 1830 ml at the 24 hour, 48 hour and one week samples. Flow rates through the SSF medium were 20 centimeters per hour for removing coliform bacteria than the sand cell. The zeolite SSFthe zeolite SSF and 12 centinte#ers per hour for the control SSF. The second coliform removal test began on June 7, when the supernatant water temperature was about 100C. The supernatant water with the sewage contained approximately 17,000 coliforms per 100 il. Samples were taken at 0, 2, 4, 6, 9, 13, 24 and 48 hours. All samples were 1500 ml in size. low rates through the SSF medium were 13 centimeters per hour for the zeolite SSF and 3 centimeters per hour for the control SSF. Analysis for coliform bacteria was performed using the membrane filter technique as given in Standard Methods for the Examination of Water and Waste Water, 15th Edition 1980, Section 909A. The concentratins of coliforms detected in the filter effluents of the March test and normalized to 100 mls are shown in Figure 4. The zeolite SSF was significantly more effective at never exceeded the drinking water standard of one coliform per 100 ml sample, maximum, while the sand cell exceeded that level from the six hour to the 24 hour test. In the coliform removal test conducted in June, two coliforms passed the polite SSF while three passed the control SSF. This was out of a total sample volume for each side of 12 liters. which equates to approximately 0.02 coliforms per 100 ml. The flow rate through the control SSF was less than one-fourth the flow rate through the zeolite SSF. Volatile Solids Study The field-scale facility was drained for scraping on the 26th of March. Before scraping. surface samples of the schmutzdecke and media were obtained from both the control SSF and the zeolite SSF using a 4.5 cm diameter plug sampler. Three different samples were taken from each SSF: (1) schmutzdecke with as little underlying medium as possible; (2) the medium immediately under the schmatzdecke but with no visible schmutzdecke included; and (3) samples of medium and schmutzdecke combined with no division at the interface. All samples were dried at l030C overnight and weighed. The samples were heated in a muffle furnace to a temperature of 5500C for one hour, cooled ip a dessicator and weighed again to determine the weight that had been driven off in the muffle furnace. Control samples of clean sand and clinoptilolite were treated similarly to determine if there was any weigt loss not attributable to biological matter. The weight loss of the 4.5 cm diameter sample plugs differed markedly between the surface of the sand and that of the clinoptilolite. The plugs from the surface of the clinoptilolite decreased in weight from 0.8 g to 2.2 g, depending on the total weight of the ashed sample which aried from 9.03 g to 25.46 g. The plugs from the surface oi the sand decreased in weight from 0.36 g to 1.14 g, while the ashed sample weighed from 4.84 g to 77.28 g. See results appearing in the graphs of Figure 5 and Figure 6. Control samples of clean sand and clinoptilolite were also ashed co determine if there was any weight loss not due to biological 4ass. The sand did not decrease in weight but the clinoptilolite decreased by 11% which appeared to be due to waters of hydration being driven off. Turbidity Removal The turbidity levels were measured for the supernatant water and the effluent of wench SSF of the facility. Samples were taken one to three times weekly from the first of October to the 20th of January and again on the 2nd of May. All samples were analyzed using a Hach Turbidimeter. For the first three weeks after the addition of the clinoptilolitn to the surface of the zeolite SSF, a large fraction of fines washed through which resulted in much higher turbialties in the effluent than in the influent (43 vei sils 3 ntu initially). Within three weeks. the turbidity in the effluent from the zeolite SSF decreased to a stable level of appioximately 30X lower than the turbidity inthe effluent from the control SSF. The turbidity of the zeolite SSF remained lower for all but one reading after that time. This was observed when flow rates through the filter were about equal, and also when the flow rates through the zeolite SSF greatly exceeded those through the control SSF. Since illfl¯nt turbidity is a critical factor influencing the length of filter cycles, it is important that the zeolite SSF also had a much longer cycle and filtered much more water between scrapings. See Figure 7. This was observed even when the control SSF had a layer of coarse sand on its surface indicating that these results are not due entirely to the coarseness of tne surface material. See Figure 8. The nature of schmutzdecke was different on the surface of the zeolite SSF from that on the control SSF. The schmutzdecke on the surface of the control SSF appeared to be incorporated into the surface of the sand and it was not possible to separate the scjmutzdecke from the sand. It fractured easily in a vertical direction and seemed to have little internal cohesion. The schmutzdecke on the surface of the zeolite, however, appeared to rest primarily on the top of the clinoptilolite and did not appear to incorporate the top layers of zeolite. It was possible to roll .back a section of the schmutzdecke almost as though it were a carpet layer on top of underlying clinoptilolite. The zeolite SSF resulted in aporoximately 30% lower turbidity.compared to the control SSF. The filter cycle was more than twice as long with the zeolite SSF and the volume of filtered water was four times greater, Since there is as much as a hundred-fold reduction in particle counts when the turbidity is reduced from 1.0 to 0.1 ntu, maintaining a lower turbidity water results in much greater safety with respect to potential pathogenic organisms. EXAMPLE 2: LABORATORY STUDIES IN GLASS COLUMNS Laboratory scale glass columns were set up to examine configurations of sand and clinoptilolite and to evaluate parameters which could not be treated in the field-scale facility due to scale or cost. Each column consisted of two joints of 15 centimeter diameter glass pie, each 152 centimeters in length. The influent water in the columns was piped in from the Logan River. Float valves were used to control the water level. Epizionteter ports were installed at 30 centimeter intervals in the lower glass pipe of ech column. Five configurations of media were placed in the columns as indicated in Figure 9. Column 1 contained a 20 centimeter layer of clinoptilolite on top of 100 centimeters of unsieved construction sand. Column 3 contained 105 centimeters of unsieved construction sand on top of a 15 centimeter layer of clinoptilolite. Column 2 was the same as Column 1 except that 20 cm of coarse sand was on top of 100 centimeters of constructioll sand. Column 4 contained a 3:1 mixture of sand : zeo 11 te. Column 5 contained on 1 y unsieved construction sand and served as a control column. Columns 1 and 3 were used to examine the removal of lead (Pb). manganese (n) and arsenic (As) from influent water. Column 5 served as a control. Metal Removal No metal ioiis were applied to the control column. The water depth over the media was maintained to 140 centimeters. The flow rate of water through the media was 20 centimeters depth per hour. or one millimeter per second. A stock solution of lead. manganese and arsenic (as lead nitrate, manganese chloride and sodium arsenate respectively) was prepared containing 6 milligrams/liter of each. A peristalt pump was used to feed this solution into columns 1 and 2 at a rate of 1 mi/minute. This flow rate was designed to produce a concentration of 100 ug/l of each of the metals in the supernatant water. This solution flowed through the columns for a period of 28 days. Influent samples were analyzed at 1 day and 14 days to compare actual influent metal concentrations to the expected metal concentration of 100 ug/l. Effluent witter sample were taken twice weekly for metal analysis. Filter flow rates were carefully maintained at 20 centimeters per hour to establish a steady state condition. After the four week metal application period. water was drained from the columns and samples of the media were removed from each column at the surface and at depths of 1 5. 30. 60. 90 and 120 centimeters. Samples were divided into three replicates and were oven dried in preparation for acid extraction. Acid extraction was performed on the three replicates of each sample. Each acid extracted replicate consisted of 20 grams of oven-dried media and 60 ml of 2 molar hydrochloric acid. Samples were shaken for 16 hours and filtered through a 0.45 micron glass filter. The replicates of each sample were then combined and analyzed for lead, manganese and arsenic using a Perkins-Elmer Induct-vely Coupled Plasma Emission Spectrophotometer (ICP). Dissolved arsenic and lead were below the detection limit using the ICP. Therefore, further analyses were performed for arsenic using the arsine gas generation technique as given in Standard Methods, supra, Section 303E to J. Lead concentrations were deter#ined using a Varian Tectron Model AA-6 Atomic Absorption Spectrophotometer. Influent and effluent manganese, lead and arsenic concentrations, in micrograns per liter, from columns 1 and 2 are listed below in Table A: Table A Column 1 Column 3 Day of Location of Mn Pb As Mn Pb As Experiment Sampl ¯ ug/l ug/l 1 Influent 92 70 76 88 45 86 12 Influent 118 25 44 139 74 83 1 Effluent < 3 2 6.6 9 < 1 1.7 5 Effluent < 3 2.5 31 < 3 < 1 54 9 Effluent 3 < 1 90 < 3 < 1 32 12 Effluent < 3 < 1 33 < 3 < 1 18 16 Effluent 19 1.2 57 < 3 1.3 39 20 Effluent 13 < 1 84 < 3 < 1 55 23 Effluent 102 < 1 80 < 3 < 1 37 28 Effluent 11 1.7 78 c3 < 1 37 Both columns were kept thoroughly stirred by air bubbling through the upper 80 centimeters of water. This stirring, combined with the supernatant detention times of approximately 7 hours, may have allowed sufficient contact time with the clinoptilolite to remove a large fraction of the lead by ion-exchange. Since the arsenic was present as an anion (HAs04), it should not have been removed by the ion-exchange with clinoptiloli+e which is a cation exchanger. Effluent metal concentrations indicate that lead was consistently removed to a level of less than 3 ug/l, which is below the federally mandated maximum of 50 micrograms per liter. Manganese levels of the effluent were less than 10 micrograms per liter for the first two weeks for both columns and remained less than 10 ug/l for column 3 to the end of the four week period. In column 1, however, the effluent manganese concentrations increased. Media concentrations in the extraction samples from the media in the columns indicate that lead and manganese were almost entirely removal in the surface layers of the columns, regardless of whether the surface layer was sand or clinoptilolite. Arsenic deposition occurred through the entire depth of the columns with the greatest concentration occurring at the surface. The results indicate that the addition of zeolite to SSF does not materially effect the removal of lead, manganese and arsenic from the influent water but the addition of a layer of clinoptilolite underheath the sand may remove a slightly higher fraction of the manganese. The Mn, As and Pb concentrations in 2 molar HCl solution extracted from the samples in the columns are listed below in ugil. Table B Sample Column 1 Column 3 Column 5 Depth (ug/1) ( ug.J 1 (us/1) Surface Mn = 123,700 Mn = 98,200 Mn = 28.560 As = 400 As = 366 As = 22 Pb = 121,000 Pb = 32,900 Pb = 1,180 15 cm Mn = 10,800 Mn = 34,590 Mn = 24,820 30 cm Mn = 29,400 Mn = 24,950 Mn = 23,920 As = 105 As = 257 As = 132 Pb = 336 Ph = 316 Pb = 160 60 cm Mn = 25,170 Mn n 24,320 Mn = 24,950 90 cm Mn = z5,840 Mn = 24,620 Mn = 26,370 120 cm Mn = 24,320 Mn = 6,100 Mn n 22,880 As = 121 As = 260 As = 65 Pb = 132 Pb = 5,500 Pb = 152 Column Head Loss Study The columns were placed in service in September. Columns 2 and 3 were scraped on the 1st of February. A compressed air system was set up by the 8th of February to bubble air up through the top 80 cm of supernatant. This was done to eliminate any possibility of greater than saturation concentrations of gases being present in the water. Bubbles had been present in the surface layers of the media, and a sample of the gas comprising the bubbles wis collected and analyzed on a gas chromatograph. The water was a#also analyzed for dissolved oxygen using the Winkler technique. On the 1 5 t of February, a record of head loss development through each column was begun by measuring the head on each pleziometer in each column three times weekly until May I and once every 7 to 14 days thereafter. Columns 1 , 3 and 5 were taken out of service on the 12th of April for metals testing. Columns 2 and 4 remained in operation. Head loss developed in the columns when they were first placed in operation in September. Visual inspection indicated that this was due to bubbles forming in the surface of the media rather than to schmutzdecke buildup. A sample of the gas comprising the bubbles was collected in early December and analyzed on a gas chromatograph. More than 99% of the sample consisted of oxygen and nitrogen, in approximately atmospheric concentrations, indicating that it was simply air, and not a product of biological activity. The columns with coarse media on the surface allowed larger bubbles to form, such that tapping on the column or column stand would cause them to escape and rise to the surface. This was apparently th: reason that the columns with a surface layer of coarse media continued to function with only small head loss during this period. At the same time that the gas samples were collected, water samples were also collected and analyzed for dissolved oxygen. The dissolved oxygen concentration in the in flu n t was found to be 9.1 mg!'l. Atmosplleric pressure in Logan, Utah , is 645 mm mercury, so dissolved oxygen sa u rat i on scld be (645/760 } x9. 5 =8.06 mg/1 . The efflllellL oxygen concentration was found to be 8.0 mg/1. Therefore, it appears that the water was entering the columns supersaturated with oxygen (and nitrogen) and that these gases were coming out of solution in the surface layers of the media. Head loss development with time at 35 cm death in the columns is shown in Figure 10, anc at 105 cm depth in Figure 11. The rapid fluctuations occurring in the first ten days of record in column 3 are a result of inaccurate adjustment of the Compressed air, which resulted in a lack of air stripping for 1-2 days on two different occasions. The rapid increases in head loss were observed to be a result of bubble formation. Head loss at 15 cm in columns 3, 4 and 5 dropped from 100+ cm on the 8th of February to a range of 12 to 27 cm on the 15th of February. This was the direct result of stripping the supersaturated air from the water, which allowed the bubbles in the surface layer of the media (at a greater depth and, therefore, pressure) to dissolve and reduce the head loss. The decrease in head loss over the first 8 days of readings at 105 cm in column 4 is due to the fact that the process of bubble dissolution was not complete when head loss readings were begun. Head loss then built up with time due to the deposition of influent turbidity and the growth of biological mass. Head loss development was much more rapid for ttic columns with normal filter sand on the surface than for the columns which had coarse media on the surface. This is to be expected, since small intergranular pore spaces will be filled more easily than large ones, either by gas, accumulated particles and schmutzdecke. An intermediate rate of head loss development occurred on the filter which consisted of a homogeneous 3:1 mixture of sand and clinoptilolite. EXAMPLE 3: ADSORPTION OF REOVIRUS TO SAND AND CLINOPTILOLITE The adsorption of reovirus type 1 to clinoptilolite and sand was compared in a 42 hour jar test. Ten grams of washed solid media and 20 ml of Logan River water filtered through a slow rate sand filter were placed in a 125 ml Erlenmeyer flask and swirled,for 42 hours at room temperature (18+2 C. Each flask contained 24r,000 counts/minute Iodine-125 labeled reovirus and one of four concentrations of infectious reovirus. Each combination of media and virus concentration was tested in triplicate. At the conclusion of 42 hours of shaking, a considerable amount of fines had been generated in both types of media. Tie fines in the clinoptilolite flasks were extremely fine, and remained in suspension even after standing guiescent for two days. The fines were separated from the clear supernatant by centrifugation for one hour at 2,500 x g. The concentrations of reovirus in the three fractions (coarse solid media. fines and clear water) were then determined by assaying for the iodine-125 labeled eovirus in each fraction. The reovirus was iodinated by the chlnramlne-T procedure described by Hunter, et al, Nature, 194 #4827):495-496 (1962). Although the level of infectious virus in the iodinated reovirus tracer was very low, the concentration of reovirus particles in the tracer was significant (lxlOE7/ml after dilution in the Logan River water). A buffer ( glycine; 0.01 M glutamic acid; 0.01 M aspartic acid; and 0.05% tween 20, pH 3.0) which elutfs reovirus from charge modified cellulose filters with an solution efficiency of 85 was used to elute reovirus from the sand and clinoptilolite. The results from the virus adsorption studies are shown in Table C below. Reovirus type 1 was found to adsorb to sand and clinoptilolite with about equal efficiencies. At the end of the 42 hour batch reactor test, 76% + 3% of the input reovirus was adsorbed to the clinoptilolite, and 81% + 2% of tie input reovirus was adsorbed to the sand. After the levels of adsorption were measured, viruses were eluted off the samples. The elution efficiency of reovirus from these solid media was quite low, 5% + 4% for the sand, and 5X + 1% for the clinoptilolite. When the adsorbed reovirus was eluted from either sand or clinoptilolite, 25% + 20% was still infectious. These results are based entirely on the physico-chemical properties of the reovirus and media. In a slow sand filter the biological properties of the schmutzdecke could greatly Increase removal efficiencies, but would not appear to reduce them, since the layers below the schmutzdecke would retain their phys ico-chemica I adsorptive properties. Table C Adsorption of Reovirus to Sand and Clinoptilolite Virus Distribution of Reovirus After 42H Particles/ % % in Coarse Media mla in Water Solids % in Fines Clinoptilolite 51x107 20 ; 4 1.6 + 0.5 78 + 9 Clinoptilolite 6.0x107 24 + 1. 3 1.3 + 0.2 75 + 8 Clinoptilolite 1.5x107 25 + 3 1.4 + 0.3 73 + 9 Clinoptilolite 1.0x107 26 # 2 i 4 + 0.3 72 + 8 Sand 51x107 17 + 2 1e + 2 67 + 8 Sand 6.0x107 18 ; 1 13 ¯ 1 63 + 7 Sand 1.5x107 20 t 2 17 + 1 63 + 4 Sand 1.0x107 20 t 2 16 + 1 64 | 9 a) Concentration of virus in Logan River Water at start of adsorption period. Robeck, et al, Journal of the American Water Works Association, 54:10:1275, (1962), showed that attenuated poliovirus Type I was removed at about 90% efficiency in a clean sand bed 60 cm deep and- at a flow rate of 4 cm/hr. When flow rates were increased to 30 cm/hr, the removal rate decreased to approximately 60%. This compares to poliovirus reductions of 3 to 5 log units in slow sand filters with sanr beds 60 cm deep reported by Poynter et al, Progress in Water Technology, 9:75 (1977). Reovirus removal rates of more than four log units were obtained in a SSF as reported by McConnell, L. K., Master's Thesis, Utah State University, Logan, Utah, (1984), after the SSF had been operating for at least one week. ghe variable results obtained in a clean sand bed compared to the coli sis tent results obtained in a SSF with an active schmutzdecke indicate that the schmutzdecke is a major factor involved in virus removal, while physico-chemical adsorption is highly dependent on factors such as virus type and water chemistry. Therefore, a zeolite, i.e., a clinoptilolite. SSF will improve virus removal since it encourages the development of a superior schmutzdecke. SAMPLE 4: BIOLOGICAL REGENERATION OF CLIOPTILOIITE A volumetric sample of 120 ml of granulated clinoptilolite was saturated with ammonium by placing the zeolite in a solution of approximately 100 grams/liter of ammonium chloride for a period of four days and agitating the mixture occasionally. The ammonium solution was then removed, and the clinoptilolite was washed three times with deionized distilled water (DDW) and divided into three equal portions of 40 ml each. Each portion was then placed in a 1000 ml beaker. The ammonium was immediately extracted from the clinoptilolite in beaker 1 by placing it in a solution of 150 grams/liter of potassium chloride and agitating it occasionally. Beaker 2 received, in addition to the 40 ml portion of clinoptilolite. 800 ml of filtered water from the Logan River and 100 ml of settled activated sludge from the Hyrum Wastewater Treatment Plant to supply ntrifying organisms. Beaker 3 received, in addition to the 40 ml portion of clinoptilolite, 900 ml of filtered Logan River water to serve as a control on beaker 2. Samples were removed from beakers 2 arid 3 it 0.25. 0.5, 1. 2, 3, 4, 6, 8 and 24 hours to be tested for nitrate concentrations. C0nCentrationS.Samples were alse removed at 0. 25, 6 and 24 hours to measure ammonium concentrations. After 24 hours, the clinoptilolite was removed from beakers 2 and 3 and te resIdual ammonium was extracted using potassium chloride. Ammonium samples were analyzed according to Standard Methods, supra, Section 417-C. Since levels of potassium chloride of 100 to 1500 mg,'l (after dilutions) were also present in the extracted samples, standards were run with and without 1500 mg/l of potassium chlolside as a control on any interference that might be present. Nitrate samples were analyzed using a Technicon Autoanalyzer 11. The supernatant in the two beakers used in the catch reactor tes (Samples 2 and 3) contained approximately equal concentrations of ammonium throughout the test. Initially, these concentrations were 46 and 47 mg/l respectively, and at both the 6 and 24 hour tests, the concentration in both supernatants was near an average of 185 mg/l. This represents a total glass of ammonium nitrogen in the supernatant of erch beaker of approximately 150 mg. After the end of the 24 hour jar tests, the remainder of the ammonium was extracted from the clinoptilolite, using potassium chloride. Both samples released 470 mg of ammonium nitrogen. When added to the amount in the supernatat, this gives a total of 620 mg released by each of samples 2 and 3. This is 12.7% higher than the control sample (Sample 1) which released 550 mg of ammonium nitrogen, but is well within the 26S standard deviation given in Standard Methods, supra, for ammonia analysis. The standards ith 1500 mg/l of potassium chloride were within 6t of those with no potassium chloride for all ammonia concentrations tested, thus there appears to be little or no potassium or chloride interference. Results from nitrate analysis of the supernatant in the two sneakers shows a marked difference. See Figure 12. The beaker with river water and activated sludge showed a decrease in nitrate concentration over the first six hours, no change from six to eight hours, and then a 430 increase from 8 to 24 hours. The beaker with river water showed only minor fluctuations in nitrate concentration, but was essentially constan-.,. never exceeding 1 mg/l. This indicates that nitrifiers were present in the sludge, but that they did not become active until some time after 8 hours. The reduction in nitrate from 0 to 6 hours may be a result of a population of nitrate using bacteria which were active during this period before nitrate prczuction began. The test was terminated at 24 hours when the ammonium in solution was approximately 40 times higher than the nitrate. Thus, it is not possible to determine whether any of the ammonium ionically bound to the clinoptilolite was utilized by the nltrlfiers or whether they used only ammonium in solution. In similar operations, the various zeolites described herein are employed In various culture mediums resulting in enhanced growth of microorganisms therein.

Claims: CLAIMS 1. In a method of culturing microorganisms in a nutrient medium, the improvement characterized by adding to the nutrient medium an amount of a zeolite effective to enhance the growth of microorganisms. 2. The method of Claim 1, characterized in that the zeolite is clinoptilolite, phillipsite or mordenite. 3. The method of Claim 1 or 2, character sized in that the nutrient medium is a slow sand filter. 4. The method of Claim 1 or 2, characterized in that the nutrient medium is e termentation broth. 5. The method of Claim 4, characterized in that the fermentation broth is a slurry broth. 6. The method of Claim 4 or 5, character--- sized in that the microorganisms are bacteria, yeast or fungi.

Inventors: CURRIER, JAMES WILLIAM, HULBERT, MATTHEW HAROLD
Applicants: INTERNATIONAL MINERALS & CHEMICAL CORPORATION
Application year: 1985
Main classification: C12N1
IPC codes: C02F3, C12N1