AMINO-PYRIMIDINE DERIVATIVES

Abstract: The invention concerns compounds of the formula I in which R¹ is lower alkyl, cyclo(lower)alkyl, phenyl(lower)al­ kyl, or trifluoromethyl; R² is oxo, thioxo or imino; R³ is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R⁴, R⁵, R⁶, R⁷ and R⁸ each independently is hydrogen or lower alkyl; R⁹ is hydrogen, lower alkenyl, 1-piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2, 3 or 4-pyri­ dinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyrid­ azinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thia­ diazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, iso­ quinolinyl, pyridopyrimidinyl, benzoxazolyl, benzimidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indol­ azinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof for use in in­ creasing cardiac contractility in mammals. Processes for their preparation and pharmaceutical compositions containing them also disclosed.

Description: This invention relates to pharmaceutical compositions containing aminopyrimidine derivatives, to the use of such derivatives and processes for preparing them, to the novel aminopyrimidine derivatives themselves, to intermediates useful in the preparation of the derivatives and to their preparation. The aminopyrimidine derivatives are useful as cardiotonic agents for increasing cardiac contractility. A number of pyrimidines and 4-oxopyrimidines are described, for example, B.Rogge et al., Chem. Abstr. 81, 25691m (1974) for East German patent 101,894, November 20, 1973; S.Kisaki et al., Chem. Pharm. Bull., 22, 2246 ('974); Derwent Publications Ltd., Farmdoc 62457W for German Offenlegenshift 2,410,650, published September 11, 1975; Derwent Publications Ltd., Farmdoc 05783J for Japanese Patent 7,176,981, published October 10, 1982; Derwent Publications Ltd., Farmdoc 10368U for Netherland Patent 7,210,637, published February 6, 1973; Chemical Abstracts, 75,49129m (1971) for Japanese Patent 7,108,698, published March 5, 1971 AKumar et al., Synthesis, (9), 748 (1980); Derwent Publications Ltd., Farmdoc 46076R for East German Patent 72,790, published May 5, 1970; and Derwent Publications Ltd., Farmdoc 31812R for British Patent 1,189,188 published November 9, 1966. The pyrimidines described in the above reports are distinguished from novel compounds of this invention by the different substituents on the pyrimidine ring and the reported biological activity. Novel amino-pyrimidine derivatives of this invention are also distinguished from the cardiotonic pyridinones, exemplified by G.Y. Lesher et al., United States Patent 4,072,746, February 7, 1978 and G.Y. Lesher et al., United States Patent 4,313,951, February 2, 1982, by having different rings and different substitution on the rings. Most of the 6-amino-5-pyrimidinecarbonitrile derivatives for which a novel use is claimed in this invention are described by Derwent Publications Ltd., Farmdoc 46076R for East German Patent 72,790, published May 5, 1970 as chemical intermediates for the production of physiologically active substances. Accordingly in one aspect this invention provides a pharmaceutical composition comprising a compound of formula I EMI2.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl(lower)alkyl, 2 is @@@ @@@@@@ @@ @@@@@ R or trifluoranethyl; R is oxo, thioxo or imino; R is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R4, R5, R6, R7 and R8 each independently is hydrogen or lower alkyl; R9 is hydrogen, lower alkenyl, 1-piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3 -furanyl, 2 or 3 -indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl rtno or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyridopyrimidinyl, benzoxazolyl, benz imidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof. In a second aspect this invention provides a compound of formula I as defined above or a therapeutically acceptable addition salt thereof for use as a pharmaceutical. The novel compounds of this invention are represented by formula I EMI3.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl(lower)alkyl, or trifluoromethyl; R2is oxo, thioxo, or imino; R3 is cyano, amino carbonyl, nitro, methylsulfonyl, aminosulfonyl; R4,R5,R6,R7and R8 each independently is hydrogen or lower alkyl; ; 9 hydrogen, lower alkenyl, 1 -piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkynyl, cyclo( lower)alkyl273 or on 4-pyridinyl 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, imidazolyl, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl isoquinolinyl, pyridopyrimidinyl, benzoxazolyl, benzimidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof with the proviso that when R1 is lower alkyl, cyclo(lower)alkyl or benzyl, R2 is oxo or thioxo; R3 is cyano; R4 is lower alkyl, and m and n are both 0 then R9 is other than hydrogen. A preferred group of compounds of this invention is represented by formula I in which R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo,thioxo or imino; R3 is cyano or aminocarbonyl; R4, R6 and R8 each independently is hydrogen or lower alkyl, R5 and R7 are hydrogen; R9 is lower alkenyl, cycl6( lower) alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 4-morpholinyl, phenyl or phenyl mono- or disubstituted with hydroxy or lower alkoxy; and m and n each independently is an integer 0 to 2; or a therapeutically addition salt thereof. A more preferred group of compounds of this invention is represented by formula I in which R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo or thioxo; R3 is cyano; R4, R5 and R7 are hydrogen; R6 and R8 each independently is hydrogen or lower alkyl; R9 is lower alkenyl, 2, 3 or 4-pyridinyl, 2-furanyl, 3indolyl, 3-thienyl, 4-morpholinyl, phenyl or phenyl mono- or disubstituted with lower alkoxy; and m and n each independently is the integer 0 or 1; or a therapeutically acceptable addition salt thereof. This invention also relates to the use for increasing cardiac contractility of a prior reported group of compounds of formula I in which R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo or thioxo; R3 is cyano; R4 is lower alkyl; R9 is hydrogen, and m and n is each the integer 0 or a therapeutically acceptable addition salt thereof. A preferred group of compounds for increasing cardiac contractility is represented by formula I in which R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo; R3 is cyano; R4 is lower alkyl; R9 is hydrogen and m and n is each the integer 0. This invention also relates to a pharmaceutical composition comprising a compound of formula I or a therapeutically acceptable addition salt thereof and a pharmaceutically acceptable carrier. This invention also relates to a method for increasing cardiac contractility in a mammal which comprises administering to the mammal an effective cardiotonic amount of a compound of formula I or a therapeutically acceptable addition salt thereof. Detailed Description of the Invention The term "lower alkyl" as used herein means straight and branched chain alkyl radicals containing from one to six carbon atoms, preferably one to four carbon atoms, and includes methyl, ethyl, propyl, l-methylethyl, butyl, 1,1dimethylethyl, pentyl and the like, unless stated otherwise. The term "lower alkenyln as used herein means straight chain alkenyl radicals containing from two to six carbon atoms and branched chain alkenyl radicals containing three to six carbon atoms and includes ethenyl, 2-methyl-2propenyl, 4-hexenyl and the like. The term lower alkynyl' as used herein means straight chain alkynyl radicals containing from two to six carbon atoms and branched chain alkynyl radicals containing four to six carbon atoms and includes ethynyl, 2-propynyl, 1methyl-2-propynyl, 3-hexynyl and the like. The term "cyclotlower)alkyl" as used herein means saturated cyclic hydrocarbon radicals containing from three to six carbon atoms and includes cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. The term "lower alkanol" as used herein means both straight and branched chain alkanols containing from one to four carbon atoms and includes methanol, ethanol, l-methylethanol, butanol and the like. Ther term "inorganic proton acceptor" as used herein means the inorganic bases, preferably the alkali metyl hydroxides, alkoxides, carbonates and bicarbonates, for example, sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, potassium carbonate, sodium hydride, sodium methoxide and the like. The compounds of this invention are capable of forming acid addition salts with therapeutically acceptable acids. The acid addition salts are prepared by reacting the base form of the appropriate compound of formula I with one or more equivalents, preferably with an excess, of the appropriate acid in an organic solvent, for example, diethyl ether or an ethanol diethyl ether mixture. These salts, when administered to a mammal, possess the same pharmacologic activities as the corresponding bases. For many purposes it is preferable to administer the salts rather than the basic compounds. Suitable acids to form these salts include the common mineral acids, e.g. hydrohalic, sulfuric or phosphoric acid; the organic acids, e.g. maleic, citric or tartaric acid; and acids which are sparingly soluble in body fluids and which impart slow-release properties to their respective salts, e.g. pamoic or tannic acid or carboxymethyl cellulose. The addition salts thus obtained are the functional equivalent of the parent base compound in respect to their therapeutic use. Hence, these addition salts are included within the scope of this invention and are limited only by the requirement that the acids employed in forming the salts be therapeutically acceptable. The compounds of formula I in which R2 is oxo or thioxo are also capable of forming addition salts with sodium or potassium. These salts are prepared by reacting the latter compounds of formula I with one or more equivalents of sodium or potassium, or a strong base of sodium or potassium, for example, sodium hydroxide, potassium t-butoxide, sodium hydride and the like. These salts, like the acid addition salts, when administered to a mammal possess the same pharmacological activities as the corresponding nonsalt compound of formula I. The compounds of formula I or a therapeutically acceptable addition salt thereof are useful as cardiotonic agents for increasing cardiac contractility in a mammal. The cardiotonic effect is demonstrated in standard pharmacological tests, for example, in causing an increase in the contractile force of the isolated cat papillary muscle and reversal of pentobarbital-induced cardiac failure in the dog. The compounds of formula I of this invention are used alone or in combination with pharmacologically acceptable carriers, the proportion of which is determined by the solubility and chemical nature of the compound, chosen route of administration and standard biological practice. For example, they are administered orally in the form of suspensions or solutions or they may be injected parenterally. For parenteral administration they can be used in the form of a sterile solution containing other solutes, for example, enough saline or glucose to make the solution isotonic. The tablet compositions contain the active ingredient in admixture with non-toxic pharmaceutical excipients known to be suitable in the manufacture of tablets. Suitable pharmaceutical excipients are, for example, starch, milk sugar, certain types of clay and so forth. The tablets can be uncoated or they can be coated by known techniques so as to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. The aqueous suspensions of the compounds of formula I contain the active ingredient in admixture with one or more non-toxic pharmaceutical excipients known to be suitable in the manufacture of aqueous suspensions. Suitable excipients are, for example, methylcellulose, sodium alginate, gum acacia, lecithin and so forth. The aqueous suspensions can also contain one or more preservatives, one or more coloring agents, one or more flavoring agents and one or more sweetening agents. Non-aqueous suspensions can be formulated by suspending the active ingredient in a vegetable oil, for example, arachis oil, olive oil, sesame oil, or coconut oil, or in a mineral oil, for example liquid paraffin, and the suspension may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. These compositions can also contain a sweetening agent, flavoring agent and antioxidant. The dosage of the compounds of formula I as cardiotonic agents will vary with the form of administration and the particular compound chosen. Furthermore, it will vary with the particular host as well as the age, weight and condition of the host under treatment as well as with the nature and extent of the symptoms. Generally, treatment is initiated with small dosages substantially less than the optimum nose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under the circumstances is reached. In general, the compounds of this invention are most desirably administered at a concentration level that will generally afford effective results without causing any harmful or deleterious side effects. For example, the effective cardiotonic amount of the compounds for oral administration can usually range from about 0.05 to 50 mg per kilogram body weight per day in single or divided doses although as aforementioned variations will occur. However, a dosage level that is in the range of from about 0.2 to 20 mg per kilogram body weight per day in single or divided doses is employed most desirably for oral administration in order to achieve effective results. The compounds of formula I also can be used to produce beneficial effects in the treatment of congestive heart failure when combined with a therapeutically effective amount of another agent commonly used in the treatment of congestive heart failure. Such agents include, for example: vasodilators, i.e. isosorbide dinitrate, captopril, nifedipine, hydralazine and prazosin; diuretics, i.e. hydrochlorothiazide, furosemide and spironolactone; and other cardiotonics, i.e. digitalis and dobutamine. A combination of the above agents can be substituted for a single agent. Suitable methods of administration, compositions and dosages of the agents are well known in the art; for instance, "Physican Desk Reference", 37 ed., Medical Economics Co., Oradell, N.J., U.S.A., 1983. When used in combination, the compound of formula I is administered as described previously. The invention also provides processes for preparing the compounds of formula I. A first process comprises reacting a pyrimidine IV EMI9.1 in which R1 is as defined above and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl with an amine V EMI9.2 in which R4 to R9, m and n are as defined above to obtain the compound corresponding to formula I in which Rê is oxo and R is cyano, nitro, methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined above. The intermediates of formula IV as defined above are also within the scope of this invention. A second process comprises condensing a compound of formula VI EMI9.3 wherein R3 is cyano, nitro, methylsulfonyl or amino sulfonyl, R4 to R91 m and n are as defined above and R10 is lower alkyl with an amidine of formula II EMI10.1 wherein R1 is as defined above to give a compound of formula I wherein R2 is oxo, R3 is cyano, nitro,methylsulfonyl or amino sulfonyl and R1, R4 to R9, m and n are as defined above. Compounds of formula I wherein X is thioxo may be prepared by reacting a compound of formula VII EMI10.2 wherein R1, R4 to R9,m and n are as defined above and R3 is cyano, nitro methylsulfonyl or amino sulfonyl, with an aqueous ethanol solution of potassium hydroxide and hydrogen sulfide. Compounds of formula VII as defined above may be reacted with a solution of an inert organic solvent containing an excess of ammonia to give a corresponding compound of formula I wherein R2is imino. Reaction scheme I illustrates a method for preparing the compounds of formula I Reaction Scheme 1 EMI11.1 I in which Rê is oxo and R is cyano, nitro, methylsulfonyl or aminosulfonyl With reference to reaction scheme 1, the amidine of formula II in which R1 is as defined herein is condensed with compound of formula III in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R10 is lower alkyl to obtain the pyrimidine of formula W in which R1 is as defined herein and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl according to the method described by S. Kisaki et al., Chem. Pharm. Bull., 22, 2246 (1974). This condensation is preferably achieved by reacting about equimolar amounts of the compounds of formulae II and m in the presence of an inorganic proton acceptor, for example, sodium hydride, sodium alkoxide or potassium carbonate, in an inert organic solvent, for example, ethanol or dimethyl formamide, at about 20 to 900 C for about two to ten hours. Reaction of the pyrimidine of formula IV in which R1 is es defined herein and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl with the amine of formula V in which R4 to R9, m and n are as defined herein gives the corresponding compound of formula I in which R2 is oxo, R3 is cyano1 nitro, methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined herein. About one to five equivalents of the amine of formula V are usually used and the reaction is conducted in an inert organic solvent, preferably l,2-dimethoxyethane, at about 50 to 1000 C for about l0 to 30 hours. Reaction Scheme 2 illustrates another method for preparing some of the compounds of formula I Reaction Scheme 2 EMI12.1 I in which R2 is oxo and R3 is cyano, nitro, methylsulfonyl oz aminosulfonyl With reference to reaction scheme 2, the compound of formula m in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R10 is lower alkyl is reacted with the amine of formula V in which R4 to R9, m and n are as defined herein to obtain the corresponding compound of formula VI in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R4 to R10, m and n are as defined herein. For this reaction, preferably about equimolar amounts of the compounds of formulae m and V are allowed to react at about 20 to 400 C for about ten minutes to two hours in an inert organic solvent, preferably dimethoxyethane, and the compound of formula VI is isolated. Condensation of the compound of formula VI in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R4 to R10, m and n are as defined herein with the amidine of formula II in which R1 is as defined herein gives the corresponding compound of formula I in which R2 is oxo; R3 is cyano, nitro, methylsulfonyl or aminosulfonyl; and R1, R4 to R9, m and n are as defined herein. When conducting this condensation, preferably about equal molar amounts of the compounds of formula II and VI are condensed at about 75 to 1500 C for about 20 to 30 hours in an inert organic solvent, preferably dim ethylformamide. To convert the compound of formula I in which R2 is oxo to the corresponding compound of formula I in which R2 is thioxo, the following chemical reactions are required. In the first step, the sodium salt of the compound of formula I in which R2 is oxo is reacted with an excess of phosphorous oxychloride at about 90 to 1200 C for about one to five hours to obtain the corresponding 4-chloropyrimidine derivative. Treatment of the latter derivative with a solution of water and ethanol containing an excess of potassium hydroxide and hydrogen sulfide at about 20 to 400 C for about 20 to 30 hours gives the corresponding compound of formula I in which R2 is thioxo; R3 is cyano, nitro, methylsulfonyl or aminosulfonyl; and R1, R4 to R9, m and n are as defined herein. Treatment of the above 4-chloropyrimidine with a solution of an inert organic solvent, preferably a lower alkanol, containing an excess of ammonia at about 50 to 800 C for about 20 to 30 hours gives the corresponding compound of formula I in which R2 is imino; R3 is cyano, nitro, methylsulfonyl or aminosulfonyl; and RÚ, R4 to R9, m and n are as defined herein. Hydrolysis of the compound of formula I in which R is cyano; and RÚ, Rê, R4 to R9, m and n are as defined herein, preferably with sulfuric acid at about 60 to 1000 C for one to ten hours, gives the corresponding compound of formula I in which R3 is aminocarbonyl; and R1, R2, R4 to R9, m and n are as defined herein. The following examples illustrate the preparation of compounds of this invention. EXAMPLE 1 1,4-Dihydro-2-methyl-6-(methylthio)-4-oxo-5-pyrimidinecarbonitrile (IV: R = Me and R3 = cyano) To a suspension of hexane washed sodium hydride (1.46 g, 50% in oil, 1.9 eq) in dimethylformamide (DMF, 1.5 mL) was added dropwise a solution of acetamidine hydrochloride (1.66 g, 1.1 eq) in DMF (7 mL). The reaction mixture was stirred at room temperature for 1 hr. A solution of 2-cyano-3, 3-bis(methylthio)-2-propenoic acid, methyl ester (described by R. Gompper et al., Chem. Ber., 95, 2861(1962), 3.24 g, 1 eq) in DMF (5 mL, prepared by warming) was added dropwise. Some reaction occurred (evident by gas evolution) during addition. The reaction mixture was allowed to stir at room temperature for 4 hr and diluted with water (13 mL). After acidification with conc. hydrochloric acid (1.5 mL), the precipitate was collected and dried (2.2 g). This was combined with 2.8 g obtained from another such batch and crystallized from DMF (25 ml)/diethyl ether (30 mL), to yield the title compound (3.8 g): mp > 2800 C and Anal. Calcd for C7H7N3OS: C, 46.39% H, 3.89% N, 23.19% and Found: C, 46.39% H, 3.79% N, 23.27%. EXAMPLE 2 a)# 1,4-Dihydro-2-methyl-4-oxo-6-[(3-pyridinylmethyl)aminol-5 -5-pyrimi dinecarb nitrile (I: RÚ = Me, Rê = O, R = CN, R4 to R8 = H, m =1, n=O and R9= 3-pyridinyl) a) To a solution of 1,4-dihydro-2-methyl-6-(methylthio)-4-oxo-5- pyrimidinecarbcnitrile (described in Example 1, 1 eq, 4.4 g) in dimethoxyethane (33 mL) was added 3-aminomethylpyridine (9.87 mL or 10.53 g, 4 eq). The reaction mixture was refluxed for 18 hours. After about 2 hr of reflux the product started to precipitate. The reaction was cooled after '18 hr and diluted with methanol (12 mL), and the precipitate was filtered. The precipitate was washed with diethyl ether, and dried to yield white solid (5,97 g). A crystallization from hot dimethylformamide yielded the title compound: mp < 260 C; Anal. Calcd for C12HllH5O: C, 59.74% H, 4.6096 N, 29.03% and found: C, 59.41% H, 4.75% N, 29.08%; IR (mineral oil) 3320, 2800, 2210, 1660 cm-1; UV max (MeOH) 289 nm ( 7250); 268 (8300); 263 (8200); 226 (40800); NMR (DMS(D-d6) # 12.2 (lH, b), 8.5 H; b), 8.45 (2H, m), 7.5 (2H, m), 4.6 (2H, d), 2.25 (3H, s). b) A suspension of the title compound (0.120 g, 1 eq) in methanol (1 mL), was stirred with methanolic hydrogen chloride (2N, 1 mL, 4 eq) added to it. The reaction mixture was stirred for 1.5 hr, diluted with diethyl ether (5 mL), and filtered. The solid was washed with diethyl ether, to yield crude product (0.16 g). Crystallization from methanol-water yielded the hydrochloride salt of the title compound (0.1 g): mp ) 250 ; and Anal. Calcd for C12H11N5O.HCl: C, 51.89% H, 4.32% N, 25.22% and Found: C, 51.81 H, 4.38% N, 25.18%. c) To a stirred suspension of the title compound (0.491 g, 1 eq) in methanol (2 mL), was added a solution of sodium in methanol (2N, 1.99 mL, 1 eq). The solid went into solution over a period of 0.5 hr. The mixture was stirred overnight during which time some precipitate appeared. Diethyl ether (3 mL) was added, and the sodium salt (0.58 g) was filtered. One crystallatization from methanol-diethyl ether yielded the sodium salt of the title compound (0.44 g): IR (mineral oil) 3660, 3630, 3420, 3320, 2850 cm 1; and UV max (MeOH) 289 nm ( #6680), 267 (7670), 263 (7570), 226 (38050). d) # q) In the same manner, but replacing 3-aminomethylpyridine with an equivalent amount of 3-N-methylaminomethylpyridine, 2-phenylethylamine, 2-(2-pyridinyl)ethylamine, 4-aminomethylpyridine, 2-aminomethylpyridine, ben zyla mine, 2-aminomethylfuran, N-(2-aminoethyl)morpholine, cyclohexanem ethylamine, 3-(2-aminoethyl)thiophene, 2-(3,4-dim ethoxyphenyl)ethylamine, 3-(2-aminoethyl)indole, (3-pyridinyl)ethylamine, (4-pyridinyl)ethylamine, the following compounds of formula I were obtained, respectively: 1,4-dihydro2-methyl-6-[N-methyl-N-(3-pyridinylmethyl)amino]-4-oxo-5-pyrimidinecarbonitrile: mp 256-258 0 C (cryst. from methanol); IR (mineral oil) 2900, 2200, 1645 cm-1; UV max (MeOH) 291 nm ( E 8490), 269 (9510), 263 (8950), 234 (32200); NMR (DMSC-d6) 6 ll.4 (br, 1H), 8.0 (m, 4H), 4.95 (s, 2H), 3.25 (s, 3H), 2.2 (s, 3H); Anal. Calcd for C13Hl3N5O: C, 61.169ó H, 5.13% N, 27.44% and Found: C, 60.84% H, 5.15% N, 27.37%; 1,4-dihydro-2-methyl-4-oxo-6 [(phenylethyl)amino]-5-pyrimidinecarbonitrile; mp 264-266 C (cryst. from methanol); NMR (DMSO-d6) 6 12.0 (br, lH), 7.8 (t, 1H), 7.2 (m, 5H), 3.55 (m, 2H), 2.8 (t, 2H), 2.2 (s, 3H); Anal. Calcd for C14H14N4O: C, 66.12% H, 5.55% N, 22.03% and Found: C, 66.11% H, 5.60% N, 21.84%; 1,4-dihydro-2- m ethyl-4-oxo6-[(2-pyridinylethyl)amino] -5-pyrimidinecarbonitrile: mp 2762780 C (cryst. from methanol); IR (mineral oil) 3310, 2850, 2220, 1673 -1 Anal. Calcd for C13H13N5O: C, 61.16% H, 5.13% N, 27.43% and Found: C, 60.92% H, 5.15% N, 27.15% 1,4-dihydro-2-methyl-4-oxo-6-[(4-pyridinylmethyl)amino3 -5-pyrimidinecarbonitrile hydrochloride: mp) 2500 C (cryst. from methanol-water); UV max (MeOH) 228nm (E 7110), 255 (8220), 225 (42790); Anal. Calcd for C12H11N5O.HCl. C, 51.90% H, 4.35% N, 25.22% and Found: C, 51.82% H, 4.43% N, 25.36%; 1,4-dihydro-2-methyl-4-oxo-6-[(2-pyridinyl methyl)amino]-5-pyrimidinecarboitrile: mp 250 C (cryst. from DMF-diethyl ether); NMR (DMSO-d6) 6 2.15 (s, 3H), 4.68 (2H), 7.2 (2 H), 7.7 (1H), 8.22 (1H), 8.47 (1H); Anal. Calcd for C12h11N5O: C, 59.74% H, 4.60% N, 29.03% and Found: C, 5.9.69% H, 4.78% N, 28.83%; 1.4-dihydro-2-methyl-4-oxo-6 [(phenylmethyl)amino] -5-pyrimidinecarbonitrile: mp 5 290 C (cryst. from DMF); NMR (DMSO-d6) 6 8.3 (t, 1H), 7.25 (s, 5H) 4.57 (d, 2H), 2.2 (s, 3H); Anal. Caled for C13Hl2N4O: C, 64.98% H, 5.04% N, 23.32% and Found: C, 64.84% H, 5.27% N, 23.28%;1,4"dihydro-6-f(2-furanylmethyl)amino]-2-methyl- 4-oxo-5-pyrimidinecarbonitrile: mp 297-300 C (cryst. from methanol); NMR (DMSO-d6) 6 2.2 (s, 3H), 4.55 (d, 2H), 6.25 (m, 2H), 7.5 (m, 1H), 8.2 (t, 1H), 11.4 (br, 1H); Anal. Calcd for C11R10N4O2: C, 57.38% H, 4.38% N, 24.34% and Found: C, 57.02% H, 4.49% N, 24.15%; 1,4-dihydro-2-methyl-6-[[2-(4- morpholinyl)ethyl]amino]-4-oxo-5-pyrimidinecarbonitrile: mp 227-230 C (cryst. from methanol-acetonitrile); NMR (CDCl3) # 2.4 (s, 3H), 2.5 (m, - 18 - -1 6H),3.65(m,6H),3.35(t,1H);ïR (mineral oil)3340,2850,1670,1595 cm 6-[(cyclohexylmethyl)amino]-1,4-dihydro-2-methyl-4-oxo-5-pyrimidinecarbonitrile.m.p. 305-308 C; NMR(DMSO-d6) of sodium salt # 1.3(m). 1.98(s), 3.1(d.J=6.5), Anal. Calcd for C13H18N4O: C, 63.39% H, 7.36% N, 22.75% and Found: C, 63.56 H, 7.32% N, 22.64%; 1,4-dihydro-2-methyl-4-oxo-6-[(2-(3-thienyl)- ethyl) amino] -5-pyrimidinecarbonitrile: mp 290-292 C; NMR (DMSOd6) 6 2.2 (s, 3H), 2.8 (t, 2H), 3.55 (m, 2H), 7.15 (m, 3H), 7.8 (t, lH); Anal. Calcd for C12H12N4OS: C, 55.36% H, 4.65% N, 21.5296 and Found: C, 55.07% H, 4.61% N, 21.36%; 1,4-dihydro-6-[[2-(3,4-dimethoxyphenyl)ethyl]amino]-2methyl-4-oxo-5-pyrimidinecarbonitrüe: mp 238-240 C (cryst. from methanol); NMR (DMSO-d6) 6 2.2 (s, 3H), 2.7 (t, 2H), 3.5 (m, 2R), 3.7 (5, 3H), 3.73 (s, 3H), 6.75 (m, 3H), 7.7 (t, lH); Anal. Calcd for C16H18NO3: C, 61.14% H, 5.77% N, 17.82% and Found: C, 60.63% H, 5.73% N, 17.68%; 1,4-dihydro-6-[[2-(1H- indol-3-yl)ethyl] amino] -2-methyl-4-oxo-5-pyrimidinecarbonitrile; mp 297 3000 C (cryst. from methanol); NMR (DMSO-d6) 6 2.2 (s, 3R), 2.9 (t, 2R), 3.6 (m, 2H), 7.1 (m, 4H), 7.85 (t, 1H), 10.75 (s, 1H), 11.5 (br, 1H); Anal. Calcd for C16 H15N5 0: C, 65.51% H, 5.11% N, 23.88% and Found: C, 65.14% H, 5.21% N, 23.78%; 1,4-dihydro-2-methyl-4-oxo-6-[2-(3-pyridinyl)ethylamino]-5-pyrimidinecarbonitrle: mp < 250 C (cryst. from DMF); UV max (MeOH) 227 nm ( E 38,300), 263 (8000), 269 (8000), 290 (6800); NMR (DMSO-d6) 6 2.2 (s, 3H), 2.85 (t, 2H), 3.6 (m, 2H), 7.25 (m, 1H), 7.6 (m, 1H), 7.85 (br, 1H), 8.4 (m, 2R); Anal. Calcd for C13H13N5O: C, 61.16% H, 5.13% N, 27.43% and Found: C, 60.62% H, 5.12% N, 27.36%; 1,4-dihydro-2-methyl-4-oxo-6-[2-(4-pyridinyl) ethylamino] -5-pyrimidinecarbonitrile: mp < 2500 C (cryst. from DMF); UV max (MeOH) 226 nm ( (# 32,700), 257 (6370), 263 (6670), 289 (6870); NMR (DMSO-d6) 6 2.2 (s, 3H), 2.82 (t, 2H), 3.6 (m, 2R), 7.18 (2d, 2H), 7.83 (br, 1H), 8.42 (2d, 2R), 11.5 (br, lH); Anal. Calcd for C13Hl3N5O: C, 61.15% H, 5.13% N, 27.43% and Found: C, 61.23% H, 5.20% N, 27.41%. EXAMPLE 3 1,4-Dihydro-2-methyl-4-oxo-6-[(2-propenyl)amino]-5-pyrimidinecarbonitrile (I: RÚ=Me, Rê=O, R =CN, R4 to R8 = H, m=1, n=0, and R9 = ethenyl) a) To a suspension of 2-cyano-3,3-bis(methylthio)-2-propenoic acid, methyl ester (8.12 g) in dimethoxyethane (13 mL) was added (2-propenyl)amine (2.51 g). The mixture dissolved. After about 15 min the solvent was evaporated under nitrogen and the solid residue was filtered with diethyl ether to give 2-cyano-3-m ethylthio-3-(2-propenyl)amino-2-propenoic acid, methyl ester (8.61 g). Similarly, but replacing (2-propenyl)amine with 3-aminopyridine, the following compound of formula VI was obtained; 2-cyano-3-methylthio- 3-(3-pyridinyl)amino-2-propenoic acid, methyl ester: mp 93-94 C. b) To a suspension of acetamidine hydrochloride (1.25 g, 1.1 eq) in DMF (45 mL) was added 2-cyano3-methylthio3-(2-propenyl)amino-2-pro- penoic acid, methyl ester (12.54 g, 1 eq) and potassium carbonate (1.82 g, 2.2 eq). The mixture was heated to 130 C for 22 hr, cooled in an ice water bath and diluted with water (20 mL). The precipitate was collected (0.7 g). The filtrate was evaporated and suspended in a small amount of water (7 mL) and filtered to give more product (0.3 g). The product was crystallized from DMF to yield the title compound (0.44 g): mp < 2600 C; UV max (MeOH) 289 nm ( # 6610), 269 (5640), 227 (42640); NMR (DMSO-d6) 6 2.2 (s, 3H), 3.95 (m, 2H), 5.05 (m, 2H), 5.8 (m, 1H), 7.9 (t, lH); Anal. Calcd for C9H10N4O: C, 56.84% H, 5.26% N, 29.47% and Found: C, 56.58% H, 5.34% N, 29.56%. c) Similarly, replacement of 2-cyano-3-m ethylthio-3-(2-propenyl)- amino-2-propenoic acid, methyl ester with 2-cyano-3-m ethylthio-3-(3-pyridinylf- amino-2-propenoic acid, methyl ester, the following compound of formula I was obtained: 1,4-dihydro-2-methyl-4-oxo-6-[(3-pyridinyl)amino]-5-pyrimidine- carbonitrile: mp) 2600 C (cryst. from DMF); IR (mineral oil) 3320, 2700, 2210, 1695 cm-1; NMR (DMSO-d6) # 2.2 (s, 3H), 7.9 (m, 4H), 9.75 (br, 2H); Anal. Calcd for C11H9N5O: C, 58.09% H, 3.99% N, 30.82% and Found: C, 57.80% H, 4.18% N, 30.36%. EXAMPLE 4 4-Amino-2-methyl-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile (I: RÚ=Me, Rê=NH, R =CN, R4 to R8 = H, m = 1, n = 0 and R9 =3- pyridinyl) a) To phosphorous oxychloride (42.9 g, or 26.1 mL, 8 eq) preheated to 1000 C (bath temp.) was added, the sodium salt of 1,4-dihydro-2-methyl- 4-oxo-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile (described in Example 2), (92 g, 1 eq). The reaction mixture was heated for 2 hr. The solution was cooled and poured into ice water (900 mL) with stirring. The resulting solution was basified with sodium hydroxide (50Si, 100 mL). Some ethyl acetate was added, and the resulting precipitate was filtered and dried to yield a solid (4.7 g). The aqueous filtrate was extracted with ethyl acetate, and the extract was dried and evaporated to give a residue (1.8 g). The combined material was crystallized from chloroform-hexane to give 4-chloro2-methyl-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile: mp 1651670 C; UV max (MeOK) 305 nm (e 4130), 254 (18100). b) A solution of the latter compound (1.5 g, 1 eq) in methanol (25 mL) was cooled in an ice bath. Ammonia was passed into the solution for 20 min. The reaction mixture turned yellow. The mixture was heated in a closed container to 60 0 C for 2 hr. The precipitate began to appear concomitently with discoloration. The mixture was stirred at room temperature overnight. The solvent was removed, water was added, and the solid was filtered to give crude product (1.4 g). Three crystallizations from DMF yielded the title compound (0.3 g): mp < 280 0 C; UV max (MeOK) 254 nm ( 12740), 234 (44070); Anal. Calcd for C12H12N6: C, 59.98% H, 5.04% N, 34.98% and Found: C, 59.50% H, 5.1396 N, 34.41%. EXAMPLE 5 1,4-Dihydro-2-methyl-6-[(3-pyridinylmethyl)amino]-4-thioxo-5-pyrimidinecarbo R1=Me, R2=5, R3=CN, R4 to 8 = H, m = 1, n = 0, and 9¯ 3-pyridinyl) To ethanol (5 mL) was added a solution of potassium hydroxide (1.5 mL, 4 molar in water-ethanol 1:9). The mixture was cooled in ice-bath. Hydrogen sulphide was passed through the solution for 15 min. 4-Chloro2-methyl-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile (described in Example 4, 0.78 g, 1 eq) in ethanol (7 mL) was added to the solution. The mixture was stirred at room temperature for 2.5 hr. Another portion of potassium hydroxide solution (1.5 mL) in ethanol (3.5 mL) was added. Hydrogen sulfide passed through for 15 min. It was stirred overnight at room temperature. The mixture was filtered and the precipitate was washed with some ethanol, to yield crude product (0.7 g). The solid was crystallized twice from DMF to yield the title compound (0.25 g): mp 310-3120 C; NMR (DMSO-a'6)0 13.2 (br, 1H), 8.8 (br, 1H), 8.5 (m, 2H), 7.5 (m, 2H), 4.6 (d, 9H), 2.3 (s, 3H); Anal. Calcd for C12HllN5S: C, 56.0196 H, 4.31% N, 27.22% and Found: C, 55.489ó H, 4.49% N, 26.68%. EXAMPLE 6 1,4-Dihydro-2-m ethyl-4-oxo-6-[(3-pyridinylm ethyl)amino] -5-pyrimidinecarboxamide (I: R1 = Me, R2 = 0, R3 = aminocarbonyl, R4 to R8 = H, m = 1, 9 n = 0, and R9 = 3-pyridinyl) Sulfuric acid (4 mL) was added to 1,4-dihydro-2-methyl-4-oxo- 6-[(3-pyridinylm ethyl)amino] -5-pyrimidinecarbonitrile (1.2 g, described in Example 2). The mixture was heated to 70 0 C for 4 hr. The reaction was cooled to room temperature, and carefully poured on crushed ice (75 mL). The resulting solution was neutralized with sodium hydroxide (50%, aqueous, 12.5 mL) when precipitate appeared. Filtrate after removal of precipitate showed the pH to be 4.5-5.0. Eight more drops of sodium hydroxide were added. More precipitate appeared. This was filtered off. The pH was checked and found to be 5. One more drop changed the pH to 8. The combined solid was dried and crystallized from boiling DMF to yield the title compound (1.1 g): mp 264-266 ; Anal Calcd for C12H14N502: C, 55.38% H, 5.38% N, 26.9% and Found: C, 55.26% H, 5.12% N, 26.70%; IR (mineral oil) 3760, 3000, 1645,1585 cm-1; NMR (DMSO-d6) # 8.5 (1H, m), 7.55 (1H, m), 7.25 (1H, m), 9;15 (1H, m), 4.7 (2H, d), 2.22 (3H, s). EXAMPLE 7 2-Methylpropanimidamide (m R1 = 1-methylethyl) Anhydrous hydrogen chloride was passed into a solution of 2methylpropanenitrile (30.4 g, 40 mL) in anhydrous ethanol (25 mL) until saturation at 250 C. The stoppered reaction mixture did not crystallize after 3 days and was evaporated to dryness under reduced pressure. The oily residue crystallized after a while and was treated with a 10% solution of anhydrous ammonia in ethanol (130 mL). After stirring for 3 hr, ammonium chloride was fntered off, and the filtrate was concentrated until crystalline. The first batch of the product (24.7 g) was collected by filtration, the second batch (9.6 g) was obtained on further concentration of the filtrate to give a total yield of the hydrochloride salt of the title compound (34.3 g): mp 155-1570 C. Similarly, by replacing 2-methylpropanenitrile with propanenitrile, 2,2-dim ethylpropanenitrile, cyclopropylnitrile, or 2-phenylethanenitrile, the following compounds of formula II were obtained respectively: propanimidamide hydrochloride (mp (130-132 C), 2,2-dimethylpropanimidamide hydro chloride (mp 186-188 C), cyclopropylm ethanimidamide hydrochloride (mp 123-1255 C), and 2-phenylethanimidamide hydrochloride (mp 145-147 C). EXAMPLE 8 1,4-Dihydro-2-(1-methylethyl)-6-(methylthio)-4-oxo-5-pyrimidinecarbonitrile (IV: R1 = 1-methylethyl) a) A solution of 2-methylpropanimidamide hydrochloride (7.0 g, described in Example 7) in dimethylformamide (15 mL) was added dropwise to a suspension of sodium hydride (3.0 g, 50% in mineral oil, prewashed with hexane) in dimethylformamide (15 mL) and the whole mixture was left at 250 C for 1 hr. 2-Cyano-3,3-bis(methylthio)-2-propenoic acid, methyl ester (6.5 g) in dimethylformamide (15 mL) was then added dropwise, and the reaction mixture was stirred for an additional 4 hr at 250 C. Water (25 mL) was then added, and acidification with concentrated hydrochloric acid (10 mL) precipitated the product. The precipitate was collected and air dried to give the title compound (6.0 g): mp < 2600 C. b) o e) Similarly, by replacing 2-methylpropanimidamide with another compound of formula described in Example 7, the following compounds of formula IV were obtained respectively: 1,4-dihydro-2-ethyl-6-(methylthio)- 4-oxo-5-pyrim i dinecarbonitrile (mp ) 260 C), 1,4-dihydro-2-(l,l-dim ethyl- ethyl6-(m ethylthio)-4-oxo- 5-pyrim idinecarbonitrile (mp 5 360 C), 2-cyclopropyl-1,4-dihydro-6-(methylthio)-4-oxo-5-pyrimidinecarbonitrile [NMR (DMSO-d6) 6 1.2 (d, 4H), 2.0 (m, 1H), 2.5 (s, 3H)], and l,4-dihydro6-(methyl- thio0-4-oxo-2-(phenylmethyl)-5-pyrimidinecarbonitrile [NMR (DMSO-d6) # 2.6 (s, 3H), 4.0 (s, 2H), 7.4 (s, 5H)]. EXAMPLE 9 1,4-Dihydro-2-(l-m ethylethylr-4-oxo-6-[(3-pyri diriylm ethyl)amino] - 5-pyrim idine carbonftrile (I: Rl = 1-methylethyl, R2 = 0, R3 = CN, R4 to R8 = H, m = 1, n = 0, and R9 = 3-pyridinyl) a) 3-(Aminomethyl)pyridine (16 mL) was added to 1,4-dihydro-2- (1-methylethyl)-6-(methylthio)-4-oxo-5-pyrimidinecarbonitrile (6 g, described in Example 8) in 1,2-dimethoxyethane (50 mL), and the mixture was refluxed overnight. Evaporation to dryness under reduced pressure gave the crude product which was chromatographed over silica acid with chloroform-methanol (97:3). The product was isolated as a colorless solid which was crystallized from methanol-water to give the title compound (3.4 g): mp < 2600 C; NMR (DMSO-d6) 6 1.1 (d, 6H), 2.7 (m, 1H), 4.55 (m, 2H), 7.3-8.4 (m, 4H), 11.5 (br, 2H); IR (mineral oil) 3335, 2900, 2210, 1660, 1585 cm-1; Anal. Calcd for C14H15- N50: C, 62.44% H, 5.61% N, 25.94% and Found: C, 62.12% H, 5.63% N, 25.92%. b) to e) Similarly, by replacing 1,4-dihydro-2-(1-methylethyl)-6-(methylthio)4-oxo-5-pyrimidinecarbonitrile with another compound of formula IV described in Example 8, the following compounds of formula I were obtained respectively: 1,4-dihydro-2-ethyl-4-oxo-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile hydrochloride: mp < 265 C (cryst. from methanol-water); NMR (DMSC-d6) 1.05 (t, 3R), 2.45 (d, 2R), 4.75 (d, 2R), 6.2 (br, 2R), 8.4 (m, 4R), 11.4 (br, lH); IR (mineral oil) 3280, 2600, 2220, 2080, 1960, 1650 cm-1; Anal. Calcd for C13H14CIN3O: C, 53.52% H, 4.84% N, 24.00% and Found: C, 53.17% H, 4.84% N, 23.74%; 1,4-dihydro-2-(1,1-dimethylethyl)-4-oxo-6-[(3-pyridinylmethyl) amino]-5-pyrimidinecarbonitrile hydrochloride: mp ) 2600 C (cryst. from methanol-water); NMR (DMSO-d6) )# # 1.17 (s, 9H), 4.15 (d, 2H), 8.5 (m, 4R), 8.6 (br, 1H), 8.9 (br, 1H), 11.9 (s, lH); IR (mineral oil) 3280, 3180, 3100, 2600, 2220, 2100, 1970, 1660 cm-1; Anal. Caled for C18R17N5O: C, 56.34% H, 5.67% N, 21.90% and Found: C, 57.74% H, 5.61% N, 21.96%; 2-cyclopropyl-1,4-dihydro- 4-oxo-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile: mp < 260 C (cryst. from 1,2-dimethoxyethane); NMR (DMSO-d6) # 1.0 (m, 4R), 1.8 (m, 1H), 4.5 (d, 2R), 7.5-8.5 (m, 4R), 12.4 (br, IR); IR (mineral oil 3300, 2850, 2190, 1685 cm-1; Anal. Calcd for C14Hl3N5O: C, 62.91% H, 4.90% N, 26.20% and Found: C, 62.31% H, 4.90% N, 25.98%; and 1,4-dihydro-4-oxo-2-(phenylmethyl)-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile: mp < 260 C (cryst. from acetonitrile); NMR (DMSO-d6) ) # 3.8 (s, 2R), 4.5 (d, 2R), 7.25 (s, 5H), 7.45 and 8.4 (m, 4H), 8.4 (br, lH); IR (mineral oil) 3330, 2800, 2210,1660, 1590 cm-l; AnaL Calcd for C18H15N5O: C, 68.13% H, 4.7696 N, 22.07% and Found: C, 67.83% H, 4.82% N, 21.85%. EXAMPLE 10 1,4-Dihydro-6-(ethylamino)-2-methyl-4-oxo-5-pyrimidinecarbonitrile (1: R1 = Me, R2 = 0, R3 = H and R4 = Et) a) To a suspension of 2-cyano-3,3-bis(methylthio)-2-propenoic acid, methyl ester (8.12 g, 1 eq) in dimethoxyethane (14 mL) was added a solution of ethylamine (2.16 g, 1.2 eq.) in dimethoxyethane (6.5 mL). The suspension turned to solution, and a white solid began to precipitate. After about 10 min the solvent was removed and the residue was filtered with diethyl ether to yield 2cyano-3-ethylamino-3-methylthio-2-propenoic acid, methyl ester (7.2 g): mp 8688 C. b) To a solution of acetamidine hydrochloride (0.83 g, 1.1 eq) in DMF (5.5 mL) was added potassium carbonate (1.22 g, 2.2 eq) and the mixture was stirred at room temperature for about 10 min. The above cyanoester (1.6 g, l eq) was added and the mixture was heated to 900 C overnight. The reaction mixture was cooled, diluted with water and filtered to give a solid (0.3 g). Further concentration to dryness followed by filtration of residue with small amount of water gave more solid (0.8 g). The two solids were pooled and crystallized from methanol to give the title compound (0.6 g): mp > 290 C; AnaL Calcd. for C8H10N4O: C, 53.93% H, 5.61% N, 31.46% and Found: C, 53.67% H, 5.83% N, 31.31%; IR (mineral oil) 3300, 2800, 2220, 1650 cm-l; UV#max (MeOH) 289 nm (± 6140), 268 (5240), 226 (40,100); NMR (DMSO-d6) Ï7.75 (t, lH), 3.4 (m, 2H), 2.23 (s, 3H), 1.1 (s, 3H). c) to flSimilarly, the following compounds of formula I can be prepared: 1,4dihydro-6-(butylamino)-2-cyclohexyl-4-oxo-5-pyrimidinecarbonitrile, 1,4dihydro-6-(diethylamino)-2-butyl-4-oxo-5-pyrimidinecarbonitrile, 1,4-dihydro-6 (N-methyl-N-jpropylamino)-2-benzyl-4-oxo-5-pyrimidinecarbonitrile, and 1,4 dihydro-6-(propylam ino)-2-cyclopropyl-4-oxo-5-pyrim idinec arboni trile. EXAMPLE 11 Test for Cardiotonic Activity in Isolated Cat Papillary Muscle A cat of either sex was anesthetized with Na pentobarbital, 25-30 mg/kg i.v. The heart was rapidly removed and placed in cool Tyrode's solution which had been equilibrated with 95% 02-5% CO2. The right ventricle was opened by cutting down the sides and around the apex so that the free wall could be folded back on the atriventricular groove. Usually at least three suitably sized papillary muscles were found (1 mm or less in thickness). Threads were tied around the chordae tendonae and the base f the muscle just above its point of insertion into the ventricular walL The chordae were cut, and the muscle was removed along with a small button of ventricular wall. If a sufficient number of papillary muscles were not present, trabeculae carnae could also be used. The best ones were usually found inserting just under the valve. The preparations were mounted in tissue baths containing Tyrode's solution at 370C bubbled with 95% 02-5% CO2. One thread was affixed to a tissue holder incorporating a pair of platinum electrodes and the other thread was attached to a force displacement transducer. Initial tension placed on the preparation was 0.2 g (less for very small muscles). The preparations were stimulated with square-wave pulses, 2-4 msec. in duration and 10% above threshold voltage, at a rate of 0.5Hz. The muscles were then gently and gradually stretched to their optimum force-length relation (at which twitch tension was maximal-further stretching did not result in any further increase in the evearall magnitude of the twitch). The preparations were then allowed to equilibrate for one hour with frequent changes of the bathing fluid (10-15 min intervals). The test compound was added to the bath in 0.1 mL of vehicle and incubated with the preparation for 15 min or until peak effect was attained. Using this method, the following representative compounds of formula 1 were effective for increasing the force of contraction of the papillary muscle (the amount of test compound in the bath and increase in contractility is given in the parenthesis): 1,4-dihydro-2-methyl-4-oxo-6-[(3-pyridinylmethyl)amino]-5pyrimidinecarbonitrile (at 10-4 molar increased contractility by 98%), 1,4dihydro-2-m ethyl-4-oxo-6-[(4-pyridinylm ethyl)am indi -5-pyrim idinecarbonitrile (at 10-4 molar increased contractility by 139%), 1,4-dihydro2-methyl-4-oxo6- [(phenylethyl)amino]-5-pyrimidinecarbonitrile (at 10-4 molar increased contractility by 142%), 1,4-dihydro-2-ethyl-4-oxo-6-[(3-pyridinylmethyl)aminod- 5-pyrimidinecarbonitrile (at 10-4 molar increased contractility by 140%), 1,4dihydro-6-[(2-furanylmethyl)amino]-2-methyl-4-oxo-5-pyrimidinecarbonitrile (at 10-4 molar increased contractility by 108%, 2-cyclopropyl-1,4-dihydro-4-oxo-6- 1(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile (at 10-4 molar increased contractility by 129%), 1,4-dihydro-2-methyl-6-[(2-(4-morpholinyl)ethyl]amino] 4-oxo-5-pyrim idinecarbonitrile (at 10-4 molar increased contractility by 6296), 1 ,4-dihydro2-m ethyl-4-oxo-6-[[2-(3-thienyl)ethyl] amino -5-pyrim idinecarbonitrile (at 10-4 molar increased contractility by 149%), 1,4-dihydro-2-methyl-4-oxo-6- [(2-propenyl)amino]-5-pyrimidinecarbonitrile (at 10-4 molar increased contractility by 61%) and 1,4-dihydro-6-(ethylamino)-2-methyl-4-ox pyrimidinecarbonitrile (at 10-4 molar increased contractility by 88%). EXAMPLE 12 Pentobarbital-induced Cardiac Failure in the Dog A dog of either sex was anesthetized with Na pentobarbital, 30-35 mg/kg i.v. The trachea was intubated and the animal was respired at a rate of 20 breaths/min (stroke volume = 15 cc/kg). Both femoral veins were cannulated. One cannula was used for infusion of pentobarbital to induce and maintain failure, the other for injection of test compounds. A cannula was inserted into the aorta via a femoral artery and the cannula was attached to a blood pressure transducer for measurement of systolic, diastolic and mean aortic blood pressure. A Millar pressure-tip catheter was inserted into the other femoral artery and advanced into the left ventricle to record intraventricular pressure and dP/dt. Subdermal needle electrodes were used to record a lead II electrocardiogram and heart rate. Following a stabilization period of at least 30 min, experimental failure was induced by the i.v. infusion of Na pentobarbital, 0.75 mg/kg/min in 0.2 mL of saline/min, until a 40-50% decrease in peak positive dP/dt was obtained. The failure state was maintained at this level throughout the experiment by continuous infusion of Na pentobarbital, 0.11-0.15 mg/kg/min. Once the maintenance infusion was started, at least 15 min were allowed to elapse before test drugs were administered. Test compounds were prepared in N saline. Increasingly higher doses were given i.v. at 30 min-lhr intervals in order to determine a therapeutic (50% increase in dP/dt) to toxic (appearance of arrhythmias) ratio where possible. Using this method, the following representative compounds of formula I were effective for increasing the cardiac contractility of the heart (the amount of test compound in mg per kg of body weight administered i.v. to give a 50% increase in dP/dt is given in the parenthesis): 1,4-dihydro-2-methyl-4-oxo-6-[(3- pyridinylmethylamino]-5-pyrimidinecarbonitrile (0.06 mg/kg), 1,4-dihydro-2m ethyl-4-oxo-6-[(4-pyridinylm ethyl)am in -5-pyrim idinecarbonitrile (0.07 mg/kg), l,4-dihydro-2-ethyl-4-oxo-6-1(3-pyridinylmethyl)amindl-5- pyrim idinecarbonitrile (0.05 mg/kg), 1,4-dihydro-6-[(2-furanylmethyl)amino] -2 m ethyl-4-oxo-5-pyrimidinecarbonitrile (0.06 mg/kg), 2-cyclopropyl-1,4-dihydro- 4-oxo-6-[(3-pyridinylm ethyl) am in -5-pyrim idinecarbonitrile (0.02 mg/kg), 1,4 dihydro-2-methyl-4-oxo-6-[[2-(3-thienyl)ethyX amind -5-pyrim idinecarbonitrile (0.08 mg/kg), and 1,4-dihydro-6-(ethylamino)-2-methyl-4-oxo-5-pyrimidine- carbonitrile (0.02 mg/kg).

Claims: CLAIMS 1. A compound of the formula I EMI27.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl (lower)alkyl, or trifluoromethyl; R2 is oxo, thioxo or imino; R3 is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R4, R5, R6, R7 and R8 each independently is hydrogen or lower alkyl; R9 is hydrogen,lower alkenyl, 1-piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-inuolylo 2 or 3thienyl, 5-imidazolyl, 4-morpholinyl, thenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazo.lyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyridopyrimidinyl, benzox- azolyl, benzimidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof for use as a pharmaceutical. 2. A pharmaceutical composition comprising a compound of formula I as defined in Claim 1 or a therapeuticaly acceptable addition salt thereof and a pharmaceutically acceptable carrier. 3. A cardiotonic pharmaceutical composition, which comprises a compound of the formula I EMI28.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl(lower)alkyl, or trifluoromethyl; R2 is oxo, thioxo or imino; R3 is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R41 R5, R6, R7 and R8 each independently is hydrogen or lower alkyl; R9 is hydrogen, lower alkenyl, 1-piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyridopyrimidinyl, benzoxazolyl, benzimidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable salt thereof and an agent commonly used in treatment of congestive heart failure. 4. A cardiotonic pharmaceutical composition as claimed in claim 3 wherein said agent is selected from isosorbide dinitrate, captopril, nifedipine, hydralazine, prazosin, hydrochlorothiazide, furosemide, spironolactone, digitalis and dobutamine. 5. A compound of the formula I EMI29.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl 2. (lower)alkyl, or trifluoromethyl; R is oxo, thioxo or imino; R3 is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R4, R5, R6, R7 and R8 each independently is hydrogen or lower alkyl; R9 is hydrogen,lower alkenyl, 1-piperidinyl, 1-pyrrolidinyle 1-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyridopyrimidinyl, benzoxazolyl, benzimidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof; with the proviso that when RÚ is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo or thioxo, R3 is cyano, R4 is lower alkyl and m and n are each 0, then R9 is other than hydrogen. 6. A compound as claimed in Claim 5 wherein R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo, thioxo or imino; R3 is cyano or aminocarbonyl; R4, R6 and R8 each independently is hydrogen or lower alkyl; R5 and R7 are hydrogen; R9 is lower alkenyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 4-morpholinyl, phenyl or phenyl mono or disubstituted with hydroxy or lower alkoxy; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof. 7. A compound as claimed in Claim 5 wherein R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo or thioxo, R is cyano; R4, R5 and R7 are hydrogen; R6 and R8 each independently is hydrogen or lower alkyl; R9 is lower alkenyl, 2,3 or 4-pyridinyl,2-furanyl, 3-indolyl, 3-thienyl, 4-morpholinyl, phenyl or phenyl mono- or disubstituted with lower alkoxy; and m and n each independently is the integer 0 or 1; or a therapeutically acceptable addition salt thereof. 8. A compound as claimed in Claim 5 which is 1,4-dihydro-2-methyl-4-oxo-6-[(3-pyridinylmethyl)amino-5- pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-6-[N-methyl- N- ( 3-pyridinylmethyl) amino] -4-oxo-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(phenylethyl)amino]-5pyrimidinecarbonitrile; 1 ,4-dihydro-2-methyl-4-oxo-6- [(2-pyridinylethyl)amino]-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(4-pyridinylmethyl)amino]-5 pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(2-pyridinylmethyl)amino]-5 pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(phenylmethyl)amino]-5- pyrimidinecarbonitrile; ,14-dihydro-2-methyl-4-oxo-6-[2-(3-pyridinyl)ethylamino] 5-pyrimidinecarbonitrile; ; ,14-dihydro-2-methyl-4-oxo-6-[2-(4-pyridinyl)ethylamino] 5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(3-pyridinyl)amino]-5- pyrimidinecarbonitrile; 1 , 4-dihydro--methyl-6- [(3- pyridinylmethyl)amino]-4-thioxo-5-pyrimidinecarbonitrile; ,14-dihydro-2-ethyl-4-oxo-6-[(3-pyridinylmethyl)amino]-5 pyrimidinecarbonitrile; ,14-dihydro-2-(1-methylethyl)-4-oxo-6-[(3-pyridinylmethyl) amino]-5-pyrimidine-carbonitrile; 2-cyclopropyl-1,4dihydro-4-oxo-6-[(3-pyridinylmethyl)amino]-5pyrimidinecarbonitrile; 1 ,4-dihydro-2-(i 1 1-dimethyl- ethyl)-4-oxo-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile; 1,4-dihydro-4-oxo-2-phenylmethyl-6 [(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile; or a pharmaceutically acceptable salt thereof. 9. A compound as claimed in Claim 5 which is 1,4-dihydro-6-[(2-furanylmethyl)amino]-2-methyl-4-oXo-5- pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-6-[[2-(4morpholinyl)ethyl]amino]-4-oxo-5-pyrimidinecarbonitrile; 1 ,4-dihydro-2-methyl-4-oxo-6-[ [2-(3-thienyl)ethyl]amino]- 5-pyrimidinecarbonitrile; 1,4-dihydro-6-[[2-(3,4dimethoxyphenyl)ethyl]amino]-2-methyl-4-oxo-5pyrimidine-carbonitrile; 1 ,4-dihydro-6-[ [2-(1H-indol-3- yl)ethyl]amino]-2-methyl-4-oxo-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(2-propenyl)amino]-5- pyrimidinecarbonitrile; or a pharmaceutically acceptable addition salt thereof. 10. A process for preparing a compound of formula I EMI31.1 in which R1 is lower alkyl, cycloflower)alkyl, phenyl (lower)alkyl, or trifluoromethyl; R2 is oxo, thioxo or imino; R3 is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R4, R5, R6, R and R each independently is hydrogen or lower alkyl; R9 is hydrogen, lower alkenyl, l-piperidinyl, l-pyrrolidinyl, l-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2,3 or 4pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyriopyrimidinyl, benzoxazolyl, benzimidazolyl, benthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof which comprises: (a) reacting a pyrimidine IV EMI32.1 in which R1 is as defined above and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl with an amine V EMI32.2 in which R4 to R9, m and n are as defined above to obtain the compound corresponding to formula I in which Rê is oxo and R is cyano, nitro,methylsulfonyl or aminosulfonyl and RÚ, R4 to R9, m and n are as defined above, or (b) reacting a compound of formula III EMI32.3 in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R10 is lower alkyl with an amine V EMI33.1 H-N-(CR5R6)m - (CR7R8)n-R9 in which R4 to R9, m and n are as defined above to obtain the compound VI EMI33.2 in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R4 to R10, m and n are as defined above, and condensing the compound VI with an amidine II EMI33.3 in which RÚ is as defined above to give a compound corresponding to formula I in which R2 is oxo, R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined above, (c) if desired converting a compound of formula I in which R2 is oxo to a compound corresponding to formula I in which R2 is thioxo by reacting a sodium salt of a compound of formula I in which R2 is oxo with phosphorous oxychloride to obtain a 4-chloropyrimidine of formula VII EMI34.1 in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined above and treating the 4--chloropyrimidine VII with an aqueous ethanol solution of potassium hydroxide and hydrogen sulfide to obtain the compound corresponding to formula I in which Rê is thioxo, R is cyano, nitro, methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined above, (d) if desired, treating a 4-chloropyrimidine VII with a solution of an inert organic solvent containing an excess of ammonia to obtain the compound corresponding to formula I in which Rê is imino, R is cyano, nitro, methylsulfonyl or aminosulfonyl, and R4 to R9, m and n are as defined above (e) if desired, hydrolyzing a compound of formula I in which R3 is cyano; and R1, R2, R4 to R9, m and n are as defined above, with sulfuric acid to obtain the compound corresponding to formula I in which R3 is aminocarbonyl; and R1, R2, R to R9, m and n are as defined above. 11. A process for preparing a compound of formula I as claimed in Claim 5 which comprises: a) condensing a compound of formula VI EMI34.2 wherein R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R4 to R9, m and n are as defined in Claim 5, and R10 is lower alkyl with an amidine of formula II EMI35.1 wherein R1 is as defined in Claim 5 to give a compound of formula I wherein R2 is oxo, R3 is cyano, nitro methylsulfonyl or aminosulfonyl and R , R to R9, m and n are as defined in Claim 5, or b) reacting a compound of formula VII EMI35.2 wherein R1, R4 to R9, m and n are as defined in Claim S and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl, with an aqueous ethanol solution of potassium hydroxide and hydrogen sulfide to give a corresponding compound of formula I wherein X is thioxo, or c) reacting a compound of formula VII as defined above with a solution of an inert organic solvent containing an excess of ammonia to give a corresponding compound of formula I wherein R2 is imino, or d) hydrolysing a compound of formula I as defined in Claim 5 wherein R3 is cyano to give a compound of formula I wherein R3 is aminocarbonyl or e) converting a compound of formula I as defined in Claim 5 to a therapeutically acceptable salt thereof. 12. A compound of formula IV EMI36.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl (lower)alkyl or trifluoromethyl, and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl. 13. A process for preparing a compound of formula IV as claimed in Claim 1 which comprises condensing a compound of formula III EMI36.2 with a compound of formula II, EMI36.3 in which formulae R1 and R3 are as defined in Claim 12 and R10 represents lower alkyl. CLAIMS FOR AUSTRIA 1. A process for preparing a compound of the formula I EMI37.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl (lower)alkyl, or trifluoromethyl; R2 is oxo, thioxo or imino; R is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R4, R5, R6, R7 and R8 each independently is hydrogen or lower alkyl; R9 is hydrogen, lower alkenyl, 1-piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkyny.l, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyridopyrimidinyl, benzoxazolyl, benzimidazolyl, benzthiazolyl, benzoxazinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof, which comprises; (a) reacting a pyrimidine IV EMI38.1 in which R1 is as defined above and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl with an amine V EMI38.2 in which R4 to R9, m and n are as defined above to obtain the compound corresponding to formula I in which R2 is oxo and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R1, R to R9, m and n are as defined above, or (b) reacting a compound of formula III EMI38.3 in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R10 is lower alkyl with an amine V EMI39.1 in which R4 to R9, m and n are as defined above to obtain the compound VI EMI39.2 in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R4 to R10, m and n are as defined above, and condensing the compound VI with an amidine II EMI39.3 in-which R1 is as defined above to give a compound corresponding to formula I in which R2 is oxo, R3is cyano, nitro, methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined above, (c) if desired converting a compound of formula I in which R2 is oxo to a compound corresponding to formula I in which R2 is thioxo by reacting a sodium salt of a compound of formula I in which Rê is oxo with phosphorous oxychloride to obtain a 4-chloropyrimidine of formula VII EMI40.1 in which R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and RÚ, R4 to R9 , m and n are as defined above and treating the 4-chloropyrimidine VII with an aqueous ethanol solution of potassium hydroxide and hydrogen sulfide to obtain the compound corresponding to formula I in which R2 is thioxo, R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and RÚ, R4 to R9, m and n are as defined above, (d) if desired, treating a 4-chloropyrimidine VII with a solution of an inert organic solvent containing an excess of ammonia to obtain the compound corresponding to formula I in which R2 is imino, R3 is cyano, nitro, methylsulfonyl or aminosulfonyl, and R1, R4 to R9, m and n are as defined above (e) if desired, hydrolyzing a compound of formula I in which R is cyano; and R1, R , R to R9, m and n are as defined above, with sulfuric acid to obtain the compound corresponding to formula I in which R3 is aminocarbonyl; and RÚ, Rê, R4 to R9, m and n are as defined above. -2. A process for preparing a compound of formula I EMI41.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl (lower)alkyl, or trifluoromethyl; R2 is oxo, thioxo or imino; R is cyano, aminocarbonyl, nitro, methylsulfonyl or aminosulfonyl; R4, R5, R6, R7 and R8 each independently is hydrogen or lower alkyl; R9is hydrogen,lower alkenyl, 1-piperidinyl, 1-pyrrolidinyl, 1-piperazinyl, lower alkynyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 5-imidazolyl, 4-morpholinyl, phenyl, phenyl mono- or disubstituted with hydroxy or lower alkoxy, 4-thiomorpholinyl, pyrazinyl, pyridazinyl, triazinyl, pyrrolyl, pyrazolyl, thiazolyl, oxadiazolyl, thiadiazolyl, isoxazolyl, furazolyl, oxathiazolyl, quinolinyl, isoquinolinyl, pyridopyrimidinyl, benzoxazolyl, benzimidazolyl, benzthiazolyl, benzoxa zinyl, benzpyronyl, isoindolyl, or indolazinyl; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof; with the proviso that when R1 is lower alkyl, cyclo(lower) 2. 3. alkyl or benzyl; R2 is oxo or thioxo, R3 is cyano, R4 is lower alkyl and m and n are each 0; then R9 is other than hydrogen, which comprises a) condensing a compound of formula VI EMI41.2 wherein R3 is cyano, nitro, methylsulfonyl or aminosulfonyl and R4 to R9, m and n are as defined herein above and R10 is lower alkyl with an amidine of formula II EMI42.1 wherein R1 is as defined hereinabove to give a compound of formula I wherein R2is oxo, R is cyano,. nitro methylsulfonyl or aminosulfonyl and R1, R4 to R9, m and n are as defined hereinabove, or b) reacting a compound of formula VII EMI42.2 wherein R1, R4 to R9, m and n are as defined hereinabove and R3 is cyano, nitro7 methylsulfonyl or aminosulfonyl, with an aqueous ethanol solution of potassium hydroxide and hydrogen sulfide to give a corresponding compound of formula I wherein X is thioxo, or c) reacting a compound of formula VII as defined above with a solution of an inert organic solvent containing an excess of ammonia to give a corresponding compound of formula I wherein R2 is imino, or d) hydrolysing a compound of formula I as defined hereinabove wherein R3 is cyano to give a compound of formula I wherein R3 is aminocarbonyl or e) converting a compound of formula I as defined hereinabove to a therapeutically acceptable salt thereof. 3. A process as claimed in Claim 1 for preparing a compound of formula I wherein R1 is lower alkyl, cyclo(lower)alkyl or benzyl; R2 is oxo, thioxo or imino; R3 is cyano or aminocarbonyl; R4, R6 and R8 each independently is hydrogen or lower alkyl; R5 and R7 are hydrogen; R9 is lower alkenyl, cyclo(lower)alkyl, 2,3 or 4-pyridinyl, 2 or 3-furanyl, 2 or 3-indolyl, 2 or 3-thienyl, 4-morpholinyl, phenyl or phenyl mono or disubstituted with hydroxy or lower alkoxy; and m and n each independently is an integer 0 to 2; or a therapeutically acceptable addition salt thereof. 4. A process as claimed in Claim 1 for preparing a compound of forrlnula I is lower alkyl.. cyclo(lower)alkyl or benzyl; R2is oxo or thioxo, R is cyano; R4, R5 and R7 are hydrogen; R and R8 each independently is hydrogen or lower alkyl: R9is lower alkenyl, 2, 3 or 4-pyridinyl.2-furanyl, 3-ina-olyl, 3-tllienylf 4-morpholinyl phenyl or phenyl mono- or disubstituted with lower alkoxy and m and n each independently is the integer 0 or 1: or a therapeutically acceptable addition salt thereof. 5. A process as claimed in Claim 1 in which the compound of formula I prepared is 1,4-dihydro-2-methyl-4-oxo-6-[(3-pyridinylmethyl)amino-5pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-6-[N-methyl N-(3-pyridinylmethyl)amino]-4-oxo-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(phenylethyl)amino]-5pyrimidinecarbonitrile; 1 ,4-dihydro-2-methyl-4-oxo-6- [(2-pyridinylethyl)amino]-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(4-pyridinylmethyl)amino]-5pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(2-pyridinylmethyl)amino]-5pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(phenylmethyl)amino]-5- pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-t2-(3-pyridinyl)ethylamino]- 5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[2-(4-pyridinyl)ethylamino]5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(3-pyridinyl)amino]-5pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-6-[(3pyridinylmethyl)amino]-4-thioxo-5-pyrimidinecarbonitrile; 1,4-dihydro-2-ethyl-4-oxo-6-[(3-pyridinylmethyl)amino]-5pyrimidinecarbonitrile; 1,4-dihydro-2-(1-methylethyl)-4-oxo-6-[(3-pyridinylmethyl)- amino]-5-pyrimidine-carbonitrile; 2-cyclopropyl-1,4- dihydro-4-oxo-6-[k(3-pyridinylmethyl)amino]-5pyrimidinecarbonitrile; 1,4-dihydro-2-(1,1-dimethyl- ethyl)-4-oxo-6-[(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrile; 1,4-dihydro-4-oxo-2-phenylmethyl-6 [(3-pyridinylmethyl)amino]-5-pyrimidinecarbonitrilei or a pharmaceutically acceptable salt thereof. 6. A process as claimed in Claim 1 in which the compound of formula I prepared is 1,4-dihydro-6-[(2-furanylmethyl)amino]-2-methyl-4-oXo-5- pyrimidinecarbonitrile; 1 ,4-dihydro-2-methyl-6-[ [2-(4- morpholinyl)ethyl]amino]-4-oxo-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[[2-(3-thienyl)ethyl]amino3- 5-pyrimidinecarbonitrile; 1,4-dihydro-6-[[2-(3,4dimethoxyphenyl)ethyl]amino]-2-methyl-4-oxo-5pyrimidine-carbonitrile; 1,4-dihydro-6-[[2-(1H-indol-3yl)ethyl]amino]-2-methyl-4-oxo-5-pyrimidinecarbonitrile; 1,4-dihydro-2-methyl-4-oxo-6-[(2-propenyl)amino]-5- pyrimidinecarbonitrile; or a pharmaceutically acceptable addition salt thereof. 7. A process for preparing a pharmaceutical composition which comprises bringing a compound of formula I as defined in Claim 1 or a therapeutically acceptable salt thereof into a form suitable for therapeutic administration. 8. A process as claimed in Claim 7 in which the composition also comprises an agent commonly used in treatment of congestive heart failure. 9. A process as claimed in Claim 8 in which said agent is selected from isosorbide dinitrate, captopril, nifedipine, hydralazine, prazosin, hydrochlorothiazide, furosemide, spironolactone, digitalis and dobutamine. 10. A process for preparing a compound of formula IV EMI46.1 in which R1 is lower alkyl, cyclo(lower)alkyl, phenyl (lower)alkyl or trifluoromethyl, and R3 is cyano, nitro, methylsulfonyl or aminosulfonyl which comprises condensing a compound of formula III EMI47.1 with a compound of formula II, EMI47.2 in which formulae R1 and R3 are as defined above and R10 represents lower alkyl.

Inventors: BAGLI, JEHAN FRAMOZ
Applicants: AMERICAN HOME PRODUCTS CORPORATION
Application year: 1984
Main classification: A61K31
IPC codes: C07D239, C07D409, C07D405, C07D401, C07D213, C07D403