A PROCESS FOR PRODUCING INHIBITING AGENTS AGAINST PROLIFERATION OF MALIGNANT TUMOUR CELLS, AND AGENTS PRODUCED THEREBY

Abstract: An inhibiting agent against the proliferation of malignant tumour cells is produced by first incubating malignant tumour cells, and then further incubating the malignant tumour cells after adding serum protein or albumin to the culture medium. The medium is isolated by removing the cells, and is then subjected to ultra-filtration through a filter for the molecular weight of 10,000. The fraction having a molecular weight of 10,000 or more is separated.

Description: A PROCESS FOR PRODUCING INHIBITING AGENTS AGAINST PROLIFERATION OF MALIGNANT TUMOUR CELLS, AND AGENTS PRODUCED THEREBY. DESCRIPTION The present invention relates to the production of an inhibiting agent against the proliferation of malignant tumour cells. We have previously proposed the production of an inhibiting agent against the proliferation of malignant tumour cells comprising the steps of incubating malignant tumour cells and, after removing the malignant tumour cells, extracting an inhibiting agent from the culture medium. We have found that inhibiting agents so produced appear not to show lethal effects against normal cells, but instead to exhibit an inhibiting activity against the proliferation of malignant tumour cells and to have a lethal effect specifically to malignant tumour cells. The present invention arises from our work seeking to obtain an agent with a significantly enhanced inhibiting effect even when provided in substantially smaller doses. In accordance with a first aspect of the present invention, we provide a process for producing an inhibiting agent against the proliferation of malignant tumour cells, characterized in that it comprises the steps of: incubating malignant tumour cells; further incubating the said. malignant tumour celTs after adding serum protein or albumin to the culture medium; isolating the culture medium by removing said malignant tumour cells; subjecting the culture medium to ultrafiltration through a filter for the molecular weight of 10,000; and separating the fraction having a molecular weight of 10,000 or more. In the preferred procedure described in more detail below, the malignant tumour cells are first allowedato proliferate in a growth medium until the incubator is saturated. The cells are transferred to an extracting medium and further Incubated after adding serum protein or albumin. The culture medium Is Isolated by removing the malignant tumour cells. Ultrafiltration Is conducted through an Amicon-filter to isolate the fraction having a molecular weight of 10,000 or more. We have found that Inhibiting agents against the prolifer ation of malignant tumour cells link with the serum or albumin in this procedure. The links in effect result in polymerization. We find that by this procedure we can obtain an inhibiting agent whose concentration is ten times or more as high as that of the inhibiting agent produced in our first proposal referred to hereinabove. Since we are now able to provide inhibiting agents concentrated by ten or more times as compared with our prior proposal, a substantially enhanced inhibition can be produced against malignant tumour cells at a significantly smaller dose. In a second and alternative aspect of the present invention, we provide an agent adapted to inhibit the proliferation of malignant tumour cells, characterized in that it has a molecular weight of 10,000 or more; in that the active agent is linked to serum protein or albumin; and in that the agent Is produced by isolating a culture medium in which malignant tumour cells have been Incubated. The Invention is hereinafter more particularly described by way of detailed illustrative examples. Example 1 For this experiment we employed an established cell line (hereinafter referred to as 11HRC") originating from a human renal carcinoma. For the growth medium we employed Basal Medium Eagle (hereinafter referred to as "BME") supplemented with 10% new born calf serum. The HRC cells were added and allowed to proliferate in an incubator to the extent that they were saturated. The serum was removed by washing once and transferred to an extracting medium where the cells were further incubated in BME supplemented with 10% new born calf serum at a temperature of between 30 C and 37 C for one week. To isolate the culture medium from the cells grown therein, the incubated culture medium was centrifuged at 3,000 rpm for 10 minutes and then filtered through a 0.45 u millipore filter. The isolated culture medium was then separated into two fractions, one having a molecular weight of 10,000 or more (hereInafter referred to a "frplO4"), and the other having a molecular weight less than 10,000 ( referred to hereinafter as "fr < 10411), using a filter for the molecular weight of 104 made by Amicon. We shall now describe the experlments which we have performed to confirm the inhibiting effects exhibited by the product resulting from Example 1. To a culture medium of BME supplemented with 10% new 4 born calf serum placed in a plastic dish, some 10 cells of HRC were inoculated. After Incubation for 24 hours, a culture medium containing the product resulting from Example 1 was exchanged for the first culture medium, and replacement of the culture medium was carried out in the same culture medium every other day. The proliferation of the cells was determined on the sixth day. Comparative tests were performed with fraction for( 104 and also wlth the tumour cell anti-proliferation agent resulting from our prior proposal referred to hereinabove and hereinafter referred to as "untreated". In the culture medium containing fr > 104, 10% or 20% new born calf serum was supplemented In order to reproduce the former concentratlon because the substances in fr),104 were considerably concentrated. On the other hand, in the culture medium containing for(104, used as a control, amino acids, vitamins and glucose, being the same ingredients and used in the same concentration as contained in BME were added to complement the nutrients consumed during extraction, and also 10% or 20% new born calf serum was added. In order to provide a comparison of effect between the agent in accordance with the present invention, the control, and the untreated agent, the survival numbers of malignant tumour cells were counted on the sixth day and the survival rate calculated as a percentage based on the cell numbers at the beginning of the determination. A measure of specific activity was produced by comparing with the untreated case after calculating the death rate by subtracting the survival rate from 100 and by calculating the death rate per unit dose by dividing the death rate by the dose employed. The experimental results set out in table 1 below were produced. Table 1 EMI5.1 <tb> Lot <SEP> Survival <SEP> Concentrated <SEP> Dose <SEP> Specific <tb> <SEP> rate <SEP> (%) <SEP> degree <SEP> (%) <SEP> activity <tb> <SEP> untreated <SEP> 0.2 <SEP> 1 <SEP> 6.0 <SEP> 1 <tb> Lot <SEP> fr > 104 <SEP> 6.6. <SEP> 17.1 <SEP> 0.35 <SEP> 16.2 <tb> 81 <SEP> fur <SEP> 57 <SEP> 1 <SEP> 6.0 <SEP> 0.43 <tb> It will be seen from Table 1 that the inhibiting agent produced by the present invention is concentrated by at least ten times when compared with the untreated agent previously proposed. Similar inhibiting effects against the proliferation of malignant tumour cells is produced at a very much smaller dose, namely less than l/lOth of that previously required. Example 2. HRC cells were added to Basal Medium Eagle (BME) supplemented with 10% new born calf serum and the malignant tumour cells were allowed to proliferate to saturate the incubator. An appropriate amount of bovine serum or bovine albumin was then added and allowed to stand at a temperature of 40C for 24 hours. The tumour cell anti-proliferation agent in accordance with the present invention was Isolated by a procedure substantially the same as that described -above with regard to Example 1. Similar tests were performed as before. The experimental results are shown in Table 2. Table 2 EMI6.1 <tb> Lot <SEP> Survival <SEP> Concentrated <SEP> Dose <SEP> Specific <tb> <SEP> rate <SEP> degree <SEP> activity <tb> <SEP> UNTREATED <SEP> 1.2 <SEP> 1 <SEP> 6 <SEP> <tb> Lot <tb> 101 <SEP> fr# <SEP> 104 <SEP> 7.2 <SEP> 18.2 <SEP> 0.33 <SEP> 17.1 <tb> A <SEP> fr#104 <SEP> <SEP> 6.7 <SEP> 0 <SEP> 6 <SEP> 1 <tb> Lot <SEP> 4 <tb> 101 <SEP> fr#104 <SEP> 10.1 <SEP> 20.0 <SEP> 0.30 <SEP> 17.8 <tb> B <SEP> fr < lO <SEP> 56 <SEP> 1 <SEP> 6 <SEP> 1 <tb>

Claims: CLAIMS 1. A process for producing an inhibiting agent against the proliferation of malignant tumour cells, characterized in that it comprises the steps of: incubating malignant tumour cells; further incubating the said malignant tumour cells after adding serum protein or albumin to the culture medium; isolating the culture medium by removing said malignant tumour cells; subjecting the culture medium to ultra-filtration through a filter for the molecular weight of 10,OOO;and separating the fraction having a molecular weight of 10,000 or more. 2. A process according to Claim 1, further characterised in that the malignant tumour cells are an established cell line originatingfrom human renal carcinoma cells. 3. A process according to Claim 1 or Claim 2, further characterized in that Basal Medium Eagle, to which 10% new born calf serum is added, is used as the culture medium. 4. A process according to any preceding claim, further charaterized in that the malignant tumour cells are first incubated in a growth medium therefor, and in that the culture medium In which the cells are further incubated after adding serum protein or albumin is an extraction medium to which the cells are transferred from said growth medium., 5. An agent adapted to Inhibit the proliferation of malignant tumour cells,characteriZed in that it has a molecular weight of 10,000 or more; in that the active agent is linked to serum protein or albumin; and in that the agent is produced by isolating a culture medium in which malignant tumour cells have been incubated. 6. Whenever produced by a process in accordance with any preceding claim, an agent adapted to Inhibit the proliferation of malignant tumour cells.

Inventors: TAJIMA, TOMOYUKI
Applicants: KOKEN LTD., NAGASU, YOUICHIRO, TAJIMA, TOMOYUKI
Application year: 1984
Main classification: A61K35
IPC codes: C12P1, A61K38, A61K35, C07K14, A61P35