GRF ANALOGS

Abstract: Human pancreatic GRF(hpGRF), rat hypothalamic GRF(rGRF) and porcine hypothalamic GRF(pGRF) have been earlier characterized and synthesized. The invention provides synthetic peptides which are extremely potent in stimulating the release of pituitary GH in animals, including humans, which have resistance to enzymatic degradation in the body, and which contain the sequence: R₁-R₂-R₃-Ala-Ile-­ Phe-Thr-R₈-Ser-R₁₀- Arg-R₁₂-R₁₃- Leu-R₁₅- Gln-R₁₇-R₁₈- Ala-­ Arg-Lys-Leu- R₂₃-R₂₄-R₂₅- Ile- R₂₇- R₂₈- Arg-Gln-Gln-Gly-Glu-­ R₃₄-Asn-Gln-Glu-R₃₈-R₃₉-R₄₀-Arg-R₄₂-R₄₃-R₄₄ wherein R₁ is a Tyr, D-Tyr, Met, Phe, D-Phe, pCl-Phe, Leu, His or D-His, which has either a Cα Me or Nα Me substitution or is unsub­ stituted; R₂ is Ala or D-Ala; R₃ is Asp or D-Asp; R₈ is Ser, Asn, D-Ser or D-Asn; R₁₀ is Tyr or D-Tyr; R₁₂ is Arg or Lys; R₁₃ is Ile or Val; R₁₅ is Gly or D-Ala; R₁₇ is Leu or D-Leu; R₁₈ is Tyr or Ser; R₂₃ is Leu or D-Leu; R₂₄ is His or Gln; R₂₅ is Glu, Asp, D-Glu or D-Asp; R₂₇ is Met, D-Met, Ala, Nle, Ile, Leu, Nva or Val; R₂₈ is Asn or Ser; R₃₄ is Arg or Ser; R₃₈ is Gln or Arg; R₃₉ is Arg or Gly; R₄₀ is Ser or Ala; R₄₂ is Phe, Ala or Val; R₄₃ is Asn or Arg; R₄₄ is a natural amino acid; provided how­ ever that either (a) R₁ has a Cα Me or an Nα Me substitution or (b) R₁₇ and/or R₂₃ is D-Leu or (c) R₂₅ is either D-Glu or D-­ Asp and provided also that any or all of the residues be­ tween R₂₉ and R₄₄, inclusive, may be deleted; or a nontoxic salt thereof. The C-terminus is preferably amidated. These peptides as well as nontoxic salts thereof may be adminis­ tered to animals, including humans and cold-blooded ani­ mals, to stimulate the release of GH and may be used diag­ nostically.

Description: GRF ANALOGS The present invention relates to peptides having influence on the function of the pituitary gland in humans and other animals. In particular, the present invention is directed to peptides which promote the release of growth hormone by the pituitary gland. Physiologists have long recognized that the hypothalamus controls the secretory functions of the adenohypophysis with the hypothalamus producing special substances which stimulate or inhibit the secretion of each pituitary hormone. In 1982, human pancreatic (tumor) releasing factors (hpGRF) were isolated from extracts of human pancreatic tumors, purified, characterized, synthesized and tested, which were found to promote the release of GH by the pituitary. A corresponding rat hypothalamic GH releasing factor (rGRF or rhGRF) and a corresponding porcine hypothalamic GH releasing factor(pGRF) have also been characterized and synthesized. Synthetic polypeptides have now been synthesized and tested which release GH from cultured pituitary cells and which may at least partially resist enzymatic degradation in the body. These peptides meet at least one of the following criteria: (a) the residue in the l-position has either a Ni Me or a Ce substitution; (b) D-Leu is present in the 17- and/or 23-positions; and/or (c) D-Glu or D-Asp is present in the 25-position. The peptides may meet more than one or even all of the foregoing criteria. The residue in the l-position may be Tyr, D-Tyr, Met, Pile, D-Phe, pCl-Phe, Leu, His and D-His (which residue may have the methyl substitution either on the alpha-carbon or in the alpha amino group). The peptides may optionally have D-Ala at the 2-position and/or D-Asp at the 3-position and/or D-Arg or D-Ser at the 8-position and/or D-Tyr at the 10-position and/or D-Ala at the 15-position. The peptides preferably also have a substitution such as D-Met, Nie or Nva for Met in the 27-position. Pharmaceutical compositions in accordance with the invention include such analogs which are between about 28 and 44 residues in length, or a nontoxic salt of any of these, dispersed in a pharmaceutically or veterinarily acceptable liquid or solid carrier. Such pharmaceutical compositions can be used in clinical medicine, both human and veterinary, for administration for therapeutic purposes, and also diagnostically. Moreover, they can be used to promote the growth of warmblooded animals, including fowl, and in aquiculture for cold-blooded animals, e.g. fish, eels, etc. The nomenclature used to define the peptides is that specified by Schroder & Lubke, "The Peptides", Academic Press (1965), wherein in accordance with conventional representation the amino group at the N-terminus appears to the left and the carboxyl group at the C-terminus to the right. By natural amino acid is meant one of common, naturally occurring amino acids found in proteins comprising Gly, Ala, Val, Leu, Ile, Ser, Thr, Lys, Arg, Asp, Asn, Glu, Gln, Cys, Met, Pile, Tyr, Pro, Trp and His. By Nle is meant norleucine, and by Nva is meant norvaline. Where the amino acid residue has isomeric forms, it is the L-form of the amino acid that is represented unless otherwise expressly indicated. The invention provides synthetic peptides having the following sequence: Rl-R2-R3-Ala-Ile-Phe-Thr-R8-Ser-RlO-Arg-Rl2- R13-Leu-R15-Gln-R17-R18-Ala-Arg-Lys-Leu-Leu- R24-R25-Ile-R27-R28-Arg-Gln-Gln-Gly-Glu-R34 Asn-Gln-Glu-R38-R39-R40-Arg-R42-R43-R44 wherein R1 is Tyr, D-Tyr, Met, Pile, D-Phe, pCl-Phe, Leu, His or D-His, which has either a C Me or NO(Me substitution or is unsubstituted; R2 is Ala or D-Alat R3 is Asp or D-Asp; R8 is Ser, Asn, D-Ser or D-Asn; Rlo is Tyr or D-Tyr; R12 is Arg or Lys; R13 is Ile or Val; R15 is Gly or D-Ala; R17 is Leu or D-Leu; R18 is Tyr or Ser; R24 is His or Gln; R25 is Glu, Asp, D-Glu or D-Asp; R27 is Met, D-Met, Ala, Nle, Ile, Leu, Nva or Val; R28 is Asn or Ser; R34 is Arg or Ser; R38 is Gln or Arg; R39 is Arg or Gly; R40 is Ser or Ala; R42 is Phe1 Ala or Val; R43 is Asn or Arg; R44 is a natural amino acid; provided however that either (a) R1 has a C < Me or an NiMe substitution or (b) R17 and/or R23 is D-Leu or (c) R25 is either D-Glu or D-Asp and provided also that any or all of the residues between R29 and R44, inclusive, may be deleted. The carboxyl moiety of the amino acid residue at the C-terminus may be the radical -COOR,-CRO,-CONHNHR,-CON(R)(R') or -CH20R, with R and R' being lower alkyl, fluoro lower alkyl or hydrogen, with methyl, ethyl and propyl being the preferred lower alkyl groups, with all of the foregoing radicals being considered to be equivalents. Usually the residue at the C-terminus is in the form of the free acid or the simple amide. When Met appears in position 1, it may be preferable to have another residue in position 27. As defined above, fragments which extend from the N-terminal through about residue-28 have biological potency, and such biologically active fragments are considered as falling within the scope of the overall invention. When the peptide fragment extends only to residue 28, Y should be NH2 or a substituted amide. When the fragment extends to one of residues 29 thru 39, Y is preferably an amide or a substituted amide but may be OH. When the fragment has 40 or more residues, there is no clear preference for the moiety at the C-terminus. The peptides are synthesized by a suitable method, such as by exclusively solid-phase techniques, by partial solid-phase techniques, by fragment condensation or by classical solution couplings. The employment of recently developed recombinant DNA techniques may be used to prepare a portion of an analog containing only natural amino acid residues. For example, the techniques of exclusively solid-phase synthesis are set forth in the textbook "Solid-Phase Peptide Synthesis", Stewart & Young, Freeman & Co., San Francisco, 1969, and are exemplified by the disclosure of U.S. Patent No. 4,105,603, issued August 8, 1978 to Vale et al. Classical solution synthesis is described in detail in the treatise "Methoden der Organischen Chemie (Houben-Weyl): Synthese von Peptiden", E. Wunsch (editor) (1974) Georg Thieme Verlag, Stuttgart, W. Ger. The fragment condensation method of synthesis is exemplified in U.S. Patent No. 3,972,859 (August 3, 1976). Other available syntheses are exemplified by U.S. Patent No. 3,842,067 (October 15, 1974) and U.S. Patent No. 3,862,925 (January 28, 1975). Common to such syntheses is the protection of the labile side chain groups of the various amino acid moieties with suitable protecting groups which will prevent a chemical reaction from occurring at that site until the group is ultimately removed. Usually also common is the protection of an alpha-amino group on an amino acid or a fragment while that entity reacts at the carboxyl group, followed by the selective removal of the alpha-amino protecting group to allow subsequent reaction to take place at that location. Accordingly, it is common that, as a step in the synthesis, an intermediate compound is produced which includes each of the amino acid residues located in its desired sequence in the peptide chain with side-chain protecting groups linked to the appropriate residues. Also considered to be within the scope of the present invention are intermediates of the formula: Xl-Rl(X or X2)-R2-R3(X3)-Ala-Ile-Phe-Thr(X4)-R8(X4 or X5)-Ser(X4 )-R10(x2)-Arg(x6 )-R12(x6 or X )-R13-Leu 5 2 6 7 R15-Gln(X5)-Rl7-Rl8(X )-Ala-Arg(X )-Lys(X )-Leu-R23- R24(X or X5)-R25(X3)-Ile-R27-R28(X4 or X5)-Arg(X6 Gln(X5)-Gln(X5)-Gly-Glu(X3)-R34(X4 or X6)-Asn(X5)- Gln(X5)-Glu(X3)-R38(X5 or X6)-R39(X6)-R40(X4)- Arg(X6)-R42-R43(X5 or X6)-R44(X8)-X9 wherein: X is either hydrogen or an amino protecting group. TheO(-amino protecting groups contemplated by X1 are those well known to be useful in the art of step-wi se synthesis of polypeptides. Among the classes of amino protecting groups which may be employed as X1 are (1) aromatic urethan-type protecting groups, such as fluorenylmethyloxycarbonyl (FMOC), benzyloxycarbonyl(Z) and substituted Z, such as p-chlorobenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, and p-methoxybenzyloxycarbonyl; (2) aliphatic urethan protecting groups, such as t-butyloxycarbonyl (BOC), diisopropylmethyloxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, allyloxycarbonyl; and (3) cycloalkyl urethan-type protecting groups1 such as cyclopentyloxycarbonyl, adamantyloxycarbonyl,and cyclohexyloxycarbonyl. The preferred Oc-amino protecting group is BOC, even when an N&alpha;Me-substituted residue is employed in the l-position. X is hydrogen or a protecting group for the imidazole nitrogen of His, such as Tos. X2 may be a suitable protecting group for the phenolic hydroxyl group of Tyr, such as tetrahydropyranyl, tert-butyl, trityl, Bzl, CBZ, 4Br-CBZ and 2,6-dichlorobenzyl(DCB). The preferred protecting 2 group is 2,6-dichlorobenzyl. X can be hydrogen which means that there is no side-chain protecting group on the amino acid residue in that position. X3 is hydrogen or a suitable ester-forming protecting group for the carboxyl group of Asp or Glu, such as benzyl(OBzl), 2,6-dichlorobenzyl, methyl and ethyl. X4 may be a suitable protecting group for the hydroxyl group of Thr or Ser, such as acetyl, benzoyl, tert-butyl, trityl, tetrahydropyranyl, Bzl, 2,6-dichlorobenzyl and CBZ. The preferred protecting group is Bzl. 4 X can be hydrogen, which means there is no protecting group on the hydroxyl group. X5 is hydrogen or a suitable protecting group for the side chain amido group of Asn or Gln. It is preferably xanthyl(Xan). X6is a suitable protecting group for the guanidino group of Arg, such as nitro, Tos, CBZ, adamantyloxycarbonyl, and BOC, or is hydrogen. 7 X is hydrogen or a suitable protecting group for the side chain amino group of Lys. Illustrative of suitable side chain amino protecting groups are 2-chlorobenzyloxycarbonyl(2-Cl-Z), Tos, t-amyloxycarbonyl and BOC. X8is hydrogen or a suitable side-chain protecting group as generally specified above. Met can optionally be protected by oxygen, but is preferably left unprotected. The selection of a side chain amino protecting group is not critical except that generally one is chosen which is not removed during deprotection of the amino groups during the synthesis. However, for some amino acids, e.g. His, protection is not generally necessary after coupling is completed, and the protecting groups may be the same. X9 is a suitable protecting group for the C-terminal carboxyl group, such as the ester-forming 3 group X , or is an anchoring bond used in solid-phase synthesis for linking to a solid resin support, or is 9 des-X , in which case the residue at the C-terminal has a carboxyl moiety which is Y, as defined hereinbefore. When such a solid resin support is used, it is broadly considered to be a protecting group and may be any of those known in therart, such as: -O-CH2-resin support, -NH-benzhydrylamine (BHA) resin support or -NH-paramethylbenzhydrylamine (MBHA) resin support. When the unsubstituted amide is desired, use of BHA or MBHA resin is preferred, because cleavage directly gives the amide. In case the N-methyl amide is desired, it can be generated from an N-methyl BHA resin. Should other substituted amides be desired or should other groups than the free acid be desired at the C-terminus, it may be preferable to sythesize the peptide using classical methods as set forth in the Houben-Weyl text. In the formula for the intermediate, at least one one of the X-groups is a protecting group or X9 includes resin support. Thus, the invention also provides a method for manufacturing a peptide of interest by (a) forming a peptide intermediate having at least one protective group and the formula (II): : X -R (X or x24)-R2-R3(x3 )-Ala-Ile-Phe-Thr(X4)-R8(X4 or X5)- Ser(X4)-Tyr(X2)-Arg(X6)-R12(X6 or X7)-R13- Leu-Gly-Gln(X )-R17-R18(X )-Ala-Arg(X )-Lys(X )-Leu- Leu-e 5 R23-Rg4(X or X )-R25(X )-Ile-R27-R28(X or X )- Arg(X6)-Gln(X5)-Gln(X5)-Gly-Glu(X3)-R34(X4 or X6 )- Asn(X5)-Gln(X5)-Glu(X3)-R38(X5 or X6)-R39(X6)- R40(X4)-Arg(X6 )-R42-R43(X5 or X6)-R44(X8)-X9 wherein: X, X1, Xê, X3, X4, X5, X6, X7 and X8 are each either hydrogen or a protective group and X9 is either a protective group or an anchoring bond to resin support or des-X9, in which case the residue at the C-terminal has a carboxy moiety which is Y; (b) splitting off the protective group or groups or anchoring bond from said peptide of the formula (II); and (c) if desired, converting a resulting peptide into a nontoxic salt thereof. In selecting a particular side chain protecting group to be used in the synthesis of the peptides, the following general rules are followed: (a) the protecting group preferably retains its protecting properties and is not be split off under coupling conditions, (b) the protecting group should be stable to the reagent and, with the exception of Xan, is preferably stable under the reaction conditions selected for removing the -amino protecting group at each step of the synthesis, and (c) the side chain protecting group must be removable, upon the completion of the synthesis containing the desired amino acid sequence, under reaction conditions that will not undesirably alter the peptide chain. When peptides are not prepared using recombinant DNA technology, they are preferably prepared using solid phase synthesis1 such as that generally described by Merrifield, J. Am. Chem. Soc., 85, p 2149 (1963), although other equivalent chemical syntheses known in the art can also be used as previously mentioned. Solid-phase synthesis is commenced from the C-terminal end of the peptide by coupling a protected amino acid to a suitable resin. Such a starting material can be prepared by attaching an O(-amino-protected amino acid by an ester linkage to a chloromethylated resin or a hydroxymethyl resin, or by an amide bond to a BHA resin or MBHA resin. The preparation of the hydroxymethyl resin is described by Bodansky et al., Chem. Ind. (London) 38, 1597-98 (1966). Chloromethylated resins are commercially available from Bio Rad Laboratories, Richmond, California and from Lab. Systems, Inc. The preparation of such a resin is described by Stewart et al., "Solid Phase Peptide Synthesis" (Freeman & Co., San Francisco 1969), Chapter 1, pp 1-6. BHA and MBHA resin supports are commercially available and are generally used only when the desired polypeptide being synthesized has an unsubstituted amide at the C-terminal. The C-terminal amino acid, i.e. Asn, protected by BOC and by Xan, can be first coupled to the chloromethylated resin according to the procedure set forth in Chemistry Letters, K. Horiki et al. 165-168 (1978), using KF in D!4F at about 60-C. for 24 hours with stirring, when the 43-residue peptide is to be synthesized. Following the coupling of the BOC-protected amino acid to the resin support, the i-amino protecting group is removed, as by using trifluoroacetic acid(TFA) in methylene chloride or TFA alone. The deprotection is carried out at a temperature between about 0 C and room temperature. Other standard cleaving reagents, such as HC1 in dioxane, and conditions for removal of specific amino protecting groups may be used as described in Schroder & Lubke, "The Peptides", 1 pp 72-75 (Academic Press 1965). After removal of the 9amino protecting group, the remainingo(-amino- and side chain-protected amino acids are coupled step-wise in the desired order to obtain the intermediate compound defined hereinbefore, or as an alternative to adding each amino acid separately in the synthesis, some of them may be coupled to one another prior to addition to the solid phase reactor. The selection of an appropriate coupling reagent is within the skill of the art. Particularly suitable as a coupling reagent is N,N'-dicyclohexyl carbodiimide (DCCI). The activating reagents used in the solid phase synthesis of the peptides are well known in the peptide art. Examples of suitable activating reagents are carbodiimides, such as N,N'-diisopropylcarbodiimide and N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide. Other activating reagents and their use in peptide coupling are described by Schroder & Lubke supra, in Chapter III and by Kapoor, J. Phar. Sci., 59, pp 1-27 (1970). I Each protected amino acid or amino acid sequence is introduced into the solid phase reactor in about a fourfold or more excess, and the coupling may be carried out in a medium of dimethylformamide(DMF): CH2C12 (1:1) or in DMF or CH2C12 alone. In cases where incomplete coupling occurs, the coupling procedure is repeated before removal of the amino protecting group prior to the coupling of the next amino acid. The success of the coupling reaction at each stage of the synthesis, if performed manually, is preferably monitored by the ninhydrin reaction, as described by E. Kaiser et al., Anal. Biochem. 34, 595 (1970). The coupling reactions can be performed automatically, as on a Beckman 990 automatic synthesizer, using a program such as that reported in Rivier et al. Biopolymers, 1978, 17, pp 1927-1938. After the desired amino acid sequence has been completed, the intermediate peptide can be removed from the resin support by treatment with a reagent, such as liquid hydrogen fluoride, which not only cleaves the peptide from the resin but also cleaves all remaining 2 3 4 5 side chain protecting groups X, X , X , X4, X5, X6, X7 and X8 and the anchoring bond X9 and also the amino protecting group X1 if one is used, to obtain the peptide in the form of the free acid. If Met is present in the sequence, the BOC protecting group is preferably first removed using trifluoroacetic acid(TFA)/ethanedithiol prior to cleaving the peptide from the resin with HF to eliminate potential S-alkylation. When using hydrogen fluoride for cleaving, anisole and methylethyl sulfide are included as scavengers in the reaction vessel. The following Example sets forth a preferred method for synthesizing peptides by the solid-phase technique. It will of course be appreciated that the synthesis of a correspondingly shorter peptide fragment is effected in the same manner by merely eliminating the requisite number of amino acids at either end of the chain; however, it is presently felt that biologically active fragments should contain the indicated sequence at the N-terminus. EXAMPLE I The synthesis of the peptide [MeHis ]- rhGRF(1-43)-OH having the formula: MeHis-Ala-Asp-Ala- Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-G1y-Gln-Leu-Tyr- Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-Gln-Gln-Gly Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-OH is conducted in a stepwise manner using a Beckman 990 peptide synthesizer on a chloromethylated resin having a substitution range of about 0.1 to 0.5 mmoles/g. resin. Coupling of BOC-Asn(Xan) to the resin is performed by the general procedure set forth in Chemistry Letters, supra, using KF in DMF at about 600C. for 24 hours with stirring, and it results in the substitution of about 0.35 mmol. Asn per gram of resin. After deblocking and neutralization, the peptide chain is built step-by-step on the resin. Deblocking, neutralization and addition of each amino acid is performed in general accordance with the procedure set forth in detail in Rivier, J, J. Amer. Chem. Soc., 96, 2986-2992 (1974). All solvents that are used are carefully degassed by sparging with an inert gas, e.g. helium or nitrogen, to insure the absence of oxygen that might undesirably oxidize the sulfur of the Met residue. Deblocking is preferably carried out in accordance with Schedule A which follows: SCHEDULE A Reagent Mixing time (Min.) 1. 60% TFA/2% ethanedithiol 10 2. 60% TFA/2% ethanedithiol 15 3. IPA/1% ethanedithiol 0.5 4. Et3N (10%) in CH2C12 0.5 5. MeOH 0.5 6. Et3N (10%) in CH2C12 0.5 7. MeOH (twice) 0.5 8. CH2C12 (twice) 0.5 The couplings are preferably carried out as set out in Schedule B which follows: SCHEDULE B Reagent Mixing time (Min.) 9. DCCI 10. Boc-amino acid 50-90 11. MeOH (twice) 0.5 12. CH2C12 (twice) 0.5 13. Ac2O (3M) in CH2C12 15.0 14. CH2C12 0.5 15. MeOH 0.5 16. CH2C12 (twice) 0.5 Briefly, one to two mmol. of BOC-protected amino acid in methylene chloride is used per gram of resin, plus one equivalent of 1.0 molar DCCI in methylene chloride for two hours. When BOC-Arg(TOS) is being coupled, a mixture of 50% DMF and methylene chloride is used. Bzl ether is used as the hydroxyl side-chain protecting group for Ser and Thr. The amido group of Asn or Gln is protected by Xan when DCC coupling is used as is preferred. P-nitrophenyl ester(OSp) may also be used to activate the carboxyl end of Asn or Gln, and for example, BOC-Asn(ONp) can be coupled overnight using one equivalent of HOBt in a 50% mixture of DMF and methylene chloride, in which case no DCC is added. 2-chloro-benzyloxyearbonyl(2Cl-Z) is used as the protecting group for the Lys side chain. Tos is used to protect the guanidino group of Arg and the imidazole nitrogen of His, and the Glu or Asp side-chain carboxyl group is protected with OBzl. The phenolic hydroxyl group of Tyr is protected with 2,6-dichlorobenzyl(DCB). At the end of the synthesis, the following composition is obtained: 4 4)Ser( 4 80C-N seHis(X)-Ala-Asp(X )-Ala-Ile-Phe-Thr(X )-Ser(X ) Ser(X43-Tyr(X2)-Arg(X6)-Arg(X63-Ile-Leu-Gly-Gln(X )- Leu-Tyr(X2 )-Ala-Arg(X6 )-Lys(X7 )-Leu-Leu-His(X)-Glu(X3)- Ile-Met-Asn(X5 )-Arg(X6 )-Gln(X5)-Gln(x5)-Gly-Glu(X3)- 6 5 5 3 5 6 Arg(X )-Asn(X )-Gin(X )-Glu(X )-Gln(X )-Arg(X )- Ser(X4 )-Arg(X6 )-Phe-Asn(X5 9 wherein X is Tos, X2 is DCB, X3 is OBzl, X is Bzl, X5 is Xan, X is Tos, X7 is 2C1-Z and X is -O-CH2-resin support. Xan may have been partially or totally removed by TFA treatment used to deblock theO(-amino protecting group. In order to cleave and deprotect the protected peptide-resin, it is treated with 1.5 ml. anisole, 0.5 ml. methylethylsulfide and 15 ml. hydrogen fluoride(HF) per gram of peptide-resin, at -20eC. for one-half hour and at o.ec. for one-half hour. After elimination of the HF under high vacuum, the resin-peptide remainder is washed alternately with dry dimethyl ether and chloroform, and the peptide is then extracted with degassed 2N aqueous acetic acid and separated from the resin by filtration. The cleaved and deprotected peptide is then dissolved in 0-5% acetic acid and subjected to purification which may include Sephadex G-50 fine gel filtration. The peptide is then further purified by preparative or semi-preparative HPLC as described in Rivier et al., Peptides: Structure and Biological Function, (1979) pp 125-8 and Marki et al. J. Am. Chem. Soc. 103, 3178 (1981). Cartridges fitting Waters Associates prep LC-500 are packed with 15-20 C18 Silica from Vydac (300A). A gradient of CH3CN in TEAP is generated by a low pressure Eldex gradient maker, as described in Rivier, J., J. Lig. Chromatography 1, 343-367 (1978). The chromatographic fractions are carefully monitored by HPLC, and only the fractions showing substantial purity are pooled. Desalting of the purified fractions, independently checked for purity, is achieved using a gradient of CH3CN in 0.1% TFA. The center cut is then lyophilized to yield the desired peptide, the purity of which can be greater than 98%. The synthesis is repeated using an MBHA resin to produce the same peptide having an amidated C-terminus using an initial procedure as generally described in Vale et al. U.S. Patent No. 4,292,313 to link Asn to the MBHA resin. EXAMPLE II The synthesis of a 40-residue amidated peptide having the formula: H-C&alpha;MeHis-Ala-Asp-Ala-Ile-Phe-Thr- Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys Leu-Leu-Hi s-Glu-I le-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn- Gln-Glu-Gln-Arg-Ser-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as generally described in Vale et al. U.S. Patent No. 4,292,313. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE III The synthesis of a rhGRF analog, i.e. [N&alpha;Me-Met1, Leu27]-rhGRF(l-43)-OH, having the formula: N&alpha;MeMet-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr- Arg-Arg-IIe-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His Glu-Ile-Leu-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln- & u-Gln- Arg-Ser-Arg-Phe-Asn-OH is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on a chloromethylated resin, in the manner generally described in Example I. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE IV The synthesis of a hpGRF analog fragment, i.e. (C"'MePhe(4C1)1]-hpGRF( 1-32)-NH2 having the formula: H-C043ePhe(4C1)-Ala-Asp-Ala-IleLPhe-Thr-Asn-Ser-Tyr-Arg- Lys-Val-Leu-Gly-G1n-Leu-Ser-Ala-Arg-Lys -Leu-Leu-G ln-Asp- Ile-Met-Ser-Arg-Gln-Gln-Gly-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. This analog is judged to be substantially pure using TLC and HPLC. The synthesis is repeated twice to produce [N&alpha;MePhe(4Cl)1]-hpGRF(1-32-NH2 and [N&alpha;MePhe1]- hpGRF( 32 )-NH2. EXAMPLE V The synthesis of a hpGRF analog fragment i.e. [N&alpha;MeTyr1]-hpGRF(1-29)-NH2 having the formula: N&alpha;MeTyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg- Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp Ile-Met-Ser-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE VI The synthesis of a rhGRF fragment i.e. [N&alpha;MePhe1]-rhGRF(1-29)-NH2, having the formula: NdMePhe-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg- Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile Met-Asn-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE VII The synthesis of [N&alpha;MeLeu1, Ile27]-hpGRF(1-32)-NH2 having the formula: N&alpha;MeLeu-Ala-Asp-Ala-Ile-Phe-Thr-Asn- Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu- Leu-Gln-Asp-Ile-Ile-Ser-Arg-Gln-Gln-Gly-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE VIII The synthesis of a rhGRF analog fragment i.e. cbLMePhe(4Cl)13-rhGRF(1-29)-NH2 having the formula: H-C!MePhe(4Cl)-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg- Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu Ile-Met-Asn-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE IX The synthesis of a rhGRF analog fragment i.e. [N&alpha;MeTyr1]-rhGRF(l-29)-NH2 having the formula: H-NiMeTyr-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg- I le-Leu-G1y-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-Hi s-Gl u-I le- Met-Asn-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE X The synthesis of [C&alpha;MeLeu1]-rhGRF-[Val44-NH2] 1 -NH23 having the formula: H-C&alpha;MeLeu-Ala-Asp-Ala-Ile-Phe-Thr- Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys- Leu-Leu-Hi s-Glu-Ile-Met-Asn-Arg-Gln-Gl n-Gly-Glu-Arg-Asn- Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-Val-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XI The synthesis of the peptide [NMeTyr1 D-Leu23 ]-rGRF(l-43)-OH having the formula: N&alpha;MeTyr- Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-IIe-Leu-Gly Gln-Leu-Tyr-Ala-Arg-Lys-Leu-D-Leu-His-Glu-Ile-Met-Asn-Arg Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-OH is conducted in a stepwise manner using a Beckman 990 peptide synthesizer on a chloromethylated resin having a substitution range of about 0.1 to 0.5 mmoles/g. resin, using the procedure set forth in EXAMPLE I. At the end of the synthesis, the following composition is obtained: BOC-N&alpha;Tyr(Xê)-Ala-Asp(X3)-Ala-Ile-Phe-Thr(X4)-Ser(X4)- Ser(X4)-Tyr(X2)-Arg(X6)-Arg(X6)-Ile-Leu-Gly-Gln(X5) Leu-Tyr(X2)- 6 7 3 )-Ala-Arg(x )-Lys(X )-Leu-D-Leu-His(X)-Glu(X )- Ile-Met-Asn(X5)-Arg(X6)-Gln(X5)-Gln(X5)-Gly-Glu(X3)- )-Arg(X6)-Gln(X5)-Gln(X5)-Gly-Glu(X3) Arg(X6)-Asn(X5)-Gln(X5)-Glu(X3)-Gln(X5)-Arg(X6)- )-Arg(X wherein )- Ser(X4)-Arg(X6)-Phe-Asn(X5)-X9 wherein X2 is DCB, X3 is OBzl, X4 is Bzl, X5 is Xan, X6 is Tos, 7 is 2C1-Z and X is -O-CH2-resin support. Xan may have been partially or totally removed by TFA treatment used to deblock the amino protecting group. The protected peptide-resin is cleaved, deprotected and purified as set forth in EXAMPLE I. Desalting of the purified fractions, independently checked for purity, is achieved using a gradient of CH3CN in 0.1% TFA. The center cut is then lyophilized to yield the desired peptide, the purity of which is greater than 98%. The synthesis is repeated using an MBHA resin to produce the same peptide having an amidated C-terminus using an initial procedure as generally described in Vale et al. U.S. Patent No. 4,292,313 to link Asn to the MBHA resin. EXAMPLE XII The synthesis of a 40-residue amidated peptide [C geHisl, D-Leu233-hpGRF(1-40)-NH2 having the formula: H-C+MeHis-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser- Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-D-Leu Gln-Asp-I 1e-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu- Arg-Gly-Ala-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as generally described in Vale et al. U.S. Patent No. 4,292,313. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XIII The synthesis of (N"Me-Met1, D-Leu , N1e27)-rGRF(1-43)-OH having the formula: NheMet-Ala- Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln Leu-Tyr-Ala-Arg-Lys-Leu-D-Leu-H is -G lu-I le-Nle-Asn-Arg- Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn- OH is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on a chloromethylated resin, in the manner generally described in Example I. The peptide is judged to be substantiallypure using TLC and HPLC. EXAMPLE XIV The synthesis of a hpGRF analog fragment, i.e. 23 [D-Leu ]-hpGRF(1-32)-NH2 having the formula: H-Tyr-Ala-Asp-Ala-I le-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-D-Leu-Gln-Asp-Ile-Met-Ser Arg-Gln-Gln-Gly-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. This analog is judged to be substantially pure using TLC and HPLC. The synthesis is repeated twice to produce [D-Leu23 D-G1u25 25]-hpGRF(1-32)-NH2 23 ]-hpGRF(l-32)-NH2 and ED-Leu Nle 1 hpGRF(1 1-32)-NH2. EXAMPLE XV The synthesis of a hpGRF analog fragment i.e. [D-Leu23, D-Asp25, Nie27 ]-hpGRF(1-29)-NH2 having the formula: H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-D-Leu-Gln D-Asp-Ile-Nle-Ser-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XVI The synthesis of ED-Leu23, N1e27 ]- rGRF(1-29)-NH2, having the formula: H-His-Ala-Asp-Ala I le-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-I le-Leu-Gly-Gln-Leu-Tyr- Ala-Arg-Lys-Leu-D-Leu-His-Glu-Ile-Nle-Asn-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. The synthesis is repeated to produce [D-Leul7, Nleê7]-rGRF(1-29)-NH2. ]-rGRF(l-29)-NH2. EXAMPLE XVII The synthesis of [D-Glu25,N1e27 )-hpGRF(l-29)-NH2 having the formula: H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn- Ser-Tyr-Arg-Lys-Val-Leu-Gly-G1n-Leu-Ser-Ala-Arg-Lys -Leu- Leu-Gln-D-Glu-Ile-Nle-Ser-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XVII I 25 27 The synthesis of [D-Glu , Nie ]- rGRF(1-29)-NH2 having the formula: H-His-Ala-Asp-Ala I le-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-I le-Leu-Gly-Gln-Leu-Tyr- Ala-Arg-Lys-Leu-Leu-His-D-Glu-Ile-Nle-Asn-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XIX 1 23 The synthesis of [D-Tyr , D-Leu ] pGRF(1-44)-NH2 having the formula: H-D-Tyr-Ala-Asp Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu- Ser-Ala-Arg-Lys-Leu-D-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln Gly-Glu-Arg-Asn-Gln-Glu-Gln-Gly-Ala-Arg-Val-Arg-Leu-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as generally described in Vale et al. U.S. Patent No. 4,292,313. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XX 1 17, 23 The synthesis of tD-His , D-Leu 25 27 Nle , Nle 1-rGRF(1-43)-OH having the formula: H-D-His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile Leu-Gly-Gln-D-Leu-Tyr-Ala-Arg-Lys-Leu-D-Leu-His-D-Glu I le-Nle-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg- Ser-Arg-Phe-Asn-OH is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on a chloromethylated resin, in the manner generally described in Example I. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XXI The synthesis of [D-Ala , D-Leu 23 25 D-Asp ]-hpGRF(1-32)-NH2 having the formula: H-Tyr- D-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu- Gly-Gln-D-Leu-Ser-Ala-Arg-Lys-Leu-D-Leu-Gln-D-Asp-Ile Met-Ser-Arg-Gln-Gln-Gly-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. This analog is judged to be substantially pure using TLC and HPLC. EXAMPLE XXII 1 2 3 The synthesis of ED-Tyr , D-Ala , D-Asp D-Asn , D-Tyr10, 15 17,23 D-Leul17,23 D A 25 D-Tyr , D-Ala15, D-Leu17,23, D-Asp 27 D-Met ]-hpGRF(1-29)-NH2 having the formula: H-D- Tyr-D-Ala-D-Asp-Ala-Ile-Phe-Thr-D-Asn-Ser-D-Tyr-Arg-Lys Val-Leu-D-Ala-Gln-D-Leu-Ser-Ala-Arg-Lys-Leu-D-Leu-Gln-D- Asp-Ile-D-Met-Ser-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. This analog is judged to be substantially pure using TLC and HPLC. EXAMPLE XXIII The synthesis of [D-His , D-Ser , 23 27]-rGRF(1-43)-OH N1e27 ]-rGRF(1-43)-OH having the formula: H-D-Hi s-Ala-Asp-Ala-Ile-Phe-Thr-D-Ser-Ser-Tyr-Arg-Arg- Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-D-Leu-His-Glu- Ile-Nle-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg- Ser-Arg-Phe-Asn-OH is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on a chloromethylated resin, in the manner generally described in Example I. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XXIV The synthesis of a rGRF analog fragment i.e. (NeTyr1, D-Glu ]-rhGRF(1-29)-NH2 having the formula: N&alpha;MeTyr-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr- Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His D-Glu-Ile-Met-Asn-Arg-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. EXAMPLE XXV The synthesis of (CMeLeu1, D-Leu , D-Glu Nle ]-rGRF-EVal44 ]-NH2having the formula: ]-NH2having H-C/MeLeu-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile- Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-D-Leu-His-D-Glu-Ile Nle-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser Arg-Phe-Asn-Val-NH2 is conducted in a stepwise manner using a Beckman 990 Peptide Synthesizer on an MBHA resin as in Example II. The peptide is judged to be substantially pure using TLC and HPLC. Various of the synthetic peptides prepared in the Examples are compared with synthetic hpGRF(1-40)-OH in in vitro assays, and all are found to exhibit generally greater potencies for the secretion of GH and similar intrinsic activities. To determine the effectiveness of several of the synthetic peptides to promote the release of growth hormone, in vitro assays are carried out using synthetic hpGRF(1-40)-OH as a standard in side-by-side comparison with equimolar concentrations of the various other analogs and fragments synthesized. Cultures are used which include cells of rat pituitary glands removed some three to five days previously. Cultures which are considered optimal for the secretion of growth hormone are used for the comparative testing, in the general manner described in Vale et al. Endocrinology, 91, 562-572 (1972) and as more particularly described in Vale et al. Endocrinology, (1983), in press. Incubation with the substance to be tested is carried out for 3 to 4 hours, and aliquots of the culture medium are removed and processed to measure their contents in immunoreactive GH(ir GH) by a well-characterized radioimmunoassay. The results of this comparative testing for equimolar concentrations are shown in Table I. TABLE I Peptide Comparison % hpGRF(1-40)-OH (standard for this test) 100% 25 27 [D-Glu , Nle ]-rGRF(l-29)-NH 31 N1e27 2 271 CD-Leu'¯, Nle rGRF(1-29)-H2 160% 17 27 [D-Leu , Nle ]-rGRF(1-29)-NH2 1 MePhe1 138% [N MePhe1]-hpGRF(1-32)-NH2 12% 1 -3; 12% Ce MePhe (4Cl)l hpGRF(1 ) NH 4.7% [4Cl-Phe ]hpGRF(1-32)-NH2 2% In vitro testing of these synthetic peptides shows that they each have the full intrinsic biological activity of hpGRF(1-40)-OH. The maximum effective 27 concentration for [D-Leu23, Nle ]-hpGRF(1-29)-NH2 is about 1 nanomolar. In addition to the in vitro tests for secretion of growth hormone, in vivo experiments inject the synthetic peptides through an indwelling catheter into freely running normal male rats after pretreating them with FLA-63, a dopamine hydroxylase inhibitor that suppresses spontaneous GH secretion without affecting the response to exogenous GRF. Blood samples are taken through the same catheter immediately prior to and 5 and 20 minutes after injections. GH levels in blood, measured by radioimmunoassay, show that synthetic 27 [D-Leu23, Nle ]-rGRF(1-29)-NH2, other D-Leu17 and D-Glu25 analogs of rGRF and the above-identified N Me and C&alpha;Me analogs of hpGRF(1-32)-NH2 are powerful stimulators of the secretion of pituitary GH and have substantially longer duration than rGRF(1-29)-NH2 and hpGRF(1-32)-NH2, respectively. Other known GRF in vivo tests, that are known to be effective to detect secretion of GH, are used to confirm these results. Dosages between about 100 nanograms and about 50 micrograms of these peptides per Kg. of body weight are considered to be effective in causing GH secretion. Such synthetic hpGRF analogs and possibly rGRF and pGRF analogs should be useful for human applications in which a physician wishes to elevate GH production. Stimulation of GH secretion by such analogs is of interest in patients with complete or relative GH deficiency caused by underproduction of endogenous GRF. Furthermore, it is probable that increased GH secretion and its attendant increase in growth could be obtained in humans or animals with normal GH levels. Moreover, administration should alter body fat content and modify other GH-dependent metabolic, immunologic and developmental processes. For example, these analogs may be useful as a means of stimulating anabolic processes in human beings under circumstances such as following the incurring of burns. As another example, these analogs may be administered to commercial warm-blooded animals, such as chickens, turkeys, pigs, goats, cattle and sheep, and may be used in aquiculture for raising fish and other cold-blooded marine animals, e.g. sea turtles and eels, and amphibians, to accelerate growth and increase the ratio of protein to fat gained by feeding effective amounts of the peptides. For administration to humans, these synthetic peptides should have a purity of at least about 93% and preferably at least 98%. Purity, for purposes of this application, refers to the intended peptide constituting the stated weight % of all peptides and peptide fragments present. For the administration of such synthetic peptides to commercial and other animals in order to promote growth and reduce fat content, a purity as low as about 5%, or even as low as 0.01%, may be acceptable. These synthetic peptides or the nontoxic salts thereof, combined with a pharmaceutically or veterinarily acceptable carrier to form a pharmaceutical composition, may be administered to animals, including humans, either intravenously, subcutaneously, intramuscularly, percutaneously, e.g. intranasally or even orally. The administration may be employed by a physician to stimulate the release of GH where the host being treated requires such therapeutic treatment. The required dosage will vary with the particular condition being treated, with the severity of the condition and with the duration of desired treatment. Such peptides are often administered in the form of nontoxic salts, such as acid addition salts or metal complexes, e.g., with zinc, iron or the like (which are considered as salts for purposes of this application). Illustrative of such acid addition salts are hydrochloride, hydrobromide, sulphate, phosphate, maleate, acetate, citrate, benzoate, succinate, malate, ascorbate, tartrate and the like. If the active ingredient is to be orally administered in tablet form, the tablet may contain a binder, such as tragacanth, corn starch or gelatin; a disintegrating agent, such as alginic acid; and a lubricant, such as magnesium stearate. If administration in liquid form is desired, sweetening and/or flavoring may be used, and intravenous administration in isotonic saline, phosphate buffer solutions or the like may be effected. The peptides should be administered to humans under the guidance of a physician, and pharmaceutical compositions will usually contain the peptide in conjunction with a conventional, solid or liquid, pharmaceutically-acceptable carrier. Usually, the parenteral dosage will be from about 100 nanograms to about 50 micrograms of the peptide per kilogram of the body weight of the host. Although the invention has been described with regard to its preferred embodiments, which constitute the best mode presently known to the inventors, it should be understood that various changes and modifications as would be obvious to one having the ordinary skill in this arty may be made without departing from the scope of the invention which is set forth in the claims appended hereto. For example, modifications in the peptide chain, particularly deletions beginning at the carboxyl terminal of the peptide, can be made in accordance with the known experimental practises to date to create peptides or peptide fragments that retain all or very substantial portions of the biological potency of the peptide, and such peptides are considered as being within the scope of the invention. Moreover, additions may be made to either terminal, or to both terminals, and/or generally equivalent residues can be substituted for naturally occurring residues, as is well-known in the overall art of peptide chemistry, to produce other analogs having at least a substantial portion of the potency of the claimed polypeptide without deviating from the scope of the invention.

Claims: Claims for the contracting states: BE, FR, DE, IT, LU, NL, SE, CH, UK. 1. A synthetic peptide having the sequence: R1-R2-R3-Ala-Ile-Phe-Thr-R8-Ser-R10-Arg-R12-R13-Leu- R15-Gln-R17 -R18-Ala-Arg-Lys-Leu-R23-R24-R25-Ile-R27 - R28-Arg-Gln-Gln-Gly-Glu-R34-Asn-Gln-Glu-R38-R39-R40 Arg-R42-R43-R44 wherein R1 is Tyr, D-Tyr, Met, Phe, D-Phe, pCl-Phe, Leu, His or D-His, which has either a CiMe or N Me substitution or is unsubstituted; R2 is Ala or D-Ala; R3 is Asp or D-Asp; R8 is Ser, Asn, D-Ser or D-Asn; Rlo is Tyr or D-Tyr; R12 is Arg or Lys; R13 is Ile or Val; R15 is Gly or D-Ala; R17 is Leu or D-Leu; R18 is Tyr or Ser; R23 is Leu or D-Leu; R24 is His or Gln; R25 is Glu, Asp, D-Glu or D-Asp; R27 is Met, D-Met, Ala, Nle, Ile, Leu, Nva or Val; R28 is Asn or Ser; R34 is Arg or Ser; R38 is Gln or Arg; R39 is Arg or Gly; R40 is Ser or Ala; R42 is Phe, Ala or Val; R43 is Asn or Arg; R44 is a natural amino acid; provided however that either (a) R1 has a C < Me or an NkMe substitution or (b) R17 and/or R23 is D-Leu or (c) R25 is either D-Glu or D-Asp and provided also that any or all of the residues between R29 and R44, inclusive, may be deleted; or a nontoxic salt thereof. 2. The peptide of claim 1 wherein R25 is D-Glu. 3. The peptide of claim 1 wherein R25 is D-Asp. 4. The peptide of claim 1, 2 or 3 wherein R23 is D-Leu. 5. The peptide of any one of claims 1 to 4 wherein R17 is D-Leu. 6. The peptide of any one of claims 1 to 5 wherein R27 is Nie. 7. The peptide of claim 1 wherein R17 is Leu, R23 is D-Leu, R25 is Glu, R27 is Nle and residues 30 through 44 are deleted. 8. The peptide of claim 7 wherein R1 is His, R2 is Ala, R3 is Asp, R8 is Ser, R10 is Tyr, R12 is Arg, R13 is Ile, R15 is Gly, R18 is Tyr, R24 is His and R28 is Asn. 9. The peptide of any one of claims 1 to 6 wherein R1 has a CRIME or N Me substitution. 10. A nontoxic salt of a peptide as claimed in any one of claims 2 to 9. 11. A pharmaceutical composition for stimulating the release of GH in an animal, comprising the peptide of any one of claims 1 to 9 or a nontoxic salt thereof, and a pharmaceutically or veterinarily acceptable liquid or solid carrier therefor. Claims for the contracting state: AT 1. A process for the manufacture of peptides having the sequence: R1-R2-R3-Ala-Ile-Phe-Thr-R8-Ser R10-Arg-R12-R13-Leu-R15-Gln-R17-R18-Ala-Arg-Lys-Leu R23-R24-R25-Ile-R27-R28-Arg-Gln-Gln-Gly-Glu-R34-Asn- Gln-Glu-R38-R39-R40 -R40-Arg-R42 -R43-R44 wherein R1 is Tyr, D-Tyr, Met, Phe, D-Phe, pCl-Phe, Leu, His or D-His, which has either a CRIME or N < Me substitution or is unsubstituted; R2 is Ala or D-Ala; R3 is Asp or D-Asp; R8 is Ser, Asn, D-Ser or D-Asn; R10 is Tyr or D-Tyr; R12 is Arg or Lys; R13 is Ile or Val; R15 is Gly or D-Ala; R17 is Leu or D-Leu; R18 is Tyr or Ser; R23 is Leu or D-Leu; R24 is His or Gln; R25 is Glu, Asp, D-Glu or D-Asp; R27 is Met, D-Met, Ala, Nle, Ile, Leu, Nva or Val; R28 is Asn or Ser; R34 is Arg or Ser; R38 is Gln or Arg; R39 is Arg or Gly; R40 is Ser or Ala; R42 is Phe, Ala or Val; R43 is Asn or Arg; R44 is a natural amino acid; provided however that either (a) R1 has a CKMe or an Ne substitution or (b) R17 and/or R23 is D-Leu or (c) R25 is either D-Glu or D-Asp and provided also that any or all of the residues between R29 and R44, inclusive, may be deleted comprising (i) forming a compound having at least one protective group and the formula (II): : X -R1(X or X2)-R2-R3(X3)-Ala-Ile-Phe-Thr(X4)-R8(X4 or X5)-Ser(X4)- R10(x2 )-Arg(X )-R2(X or X )-R13-Leu-Rl5-Gln(X )-R17-3 R18(X )-Ala-Arg(X )-Lys(X )-Leu-R23-R24(X or X )-R25(X 25 5 6 5 5 3 Ile-R27-R28(X4 or X )-Arg(X )-Gln(X )-Gln(X )-Gly-Glu(X )- R3(x or X6)-Asn(X5)-Gln(X5)-Glu(X3)-R38(X5 or x6)-R (X )-R407(x4)- wherein (X), (X ), (X2), (x3), (X ), (X ), and (X7) and (X8) are each either hydrogen or a protec 9 tive group and wherein (X9) is either amide or hydroxyl or a protective group, and (ii) splitting off the protective group or groups from said compound of the formula (II) to provide a peptide having the formula (I) or a biologically active fragment thereof and, if desired, (iii) converting the resulting peptide into a nontoxic addition salt thereof. 2. A process in accordance with Claim 1 wherein R25 is D-Glu. 3. A process in accordance with Claim 1 wherein R25 is D-Asp. 4. A process in accordance with Claims 1, 2 or 3 wherein R23 is D-Leu. 5. A process in accordance with any one of Claims 1-4 wherein R17 is D-Leu. 6. A process in accordance with any one of Claims 1 to 5 wherein R27 is Nle. 7. A process in accordance with any one of Claims 1 to 6 wherein R1 has a C > ie or N 9e substitution. 8. A process in accordance with any one of Claims 1 to 7 wherein X1 is hydrogen or an amino 2 protecting group; X is hydrogen or a protecting group 3 for the phenolic hydroxyl group of Tyr; X is hydrogen or a protecting group for the carboxyl group Asp or Glu; 4 X is hydrogen or a protecting group for the alcoholic hydroxyl group of Thr: X5is hydrogen or a protecting group for the alcoholic hydroxyl group of Ser; X6 is hydrogen or a protecting group for the guanidino group 7 of Arg; X7 is hydrogen or a protecting group for the 8 side chain amino group of Lys; X is hydrogen or a suitable side-chain protecting group; X is hydrogen or a protecting group for the imidazole nitrogen of His; and X9 is selected from the class consisting of OH, OCH3, esters, amides, hydrazides, -O-CH2-resin support and -NH-resin support. 9. A process in accordance with Claim 8 wherein X1 is BOC, x2 is 2,6-dichlorobenzyl, X3 is Bzl, X4 is Bzl, X5 is Bzl, X6 is Tos, X7 is 2-chlorobenzyloxycarbonyl and X is -O-CH2-resin support. 10. A process in accordance with Claim 8 wherein 3. wherein X1 is BOC, X2 is 2,6-dichlorobenzyl, X is Bzl, X4 is Bzl, X is Bzl, X is Tos, X7 is 2-chlorobenzyloxycarbonyl and X9 is -NH-resin support.

Inventors: RIVIER, JEAN EDOUARD FREDERIC, VALE, WYLIE WALKER, JR.
Applicants: THE SALK INSTITUTE FOR BIOLOGICAL STUDIES
Application year: 1984
Main classification: C07K7
IPC codes: C07K14, A61K38, C12P21, A23K1