IMMUNOASSAY FOR HUMAN CHORIONIC GONADOTROPIN

Abstract: An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the beta subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the beta subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is detectable when both are bound to hCG. In a presently preferred embodiment, an immunoassay for hCG or hCG beta in urine includes a purified, labeled or detectable serum-derived antibody directed to the carboxy-terminal portion of the beta subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the beta subunit sufficiently remote from the carboxy-terminal portion that both antibodies can simultaneously bind to hCG or hCG beta .

Description: IMMUNOASSAY FOR HUMAN CHORIONIC GONADOTROPIN The invention described herein was made in the course of work under grant numbers HD-15454 and RR-00645 from the National Institutes of Health, United States Department of Health and Human Services, U.S.A. Background of the Invention Throughout this application various publications are re¬ ferred to to provide background information useful for a complete understanding of the invention. The disclosures of these references are hereby incorporated in their entireties into * the present application. Immunoassays for human chorionic gonadotropin (hCG) are known and have been widely used, particularly in the diagnosis of pregnancy. Recent advances in immunology involving hybridomas and monoclonal antibodies have greatly increased the sensitivity of previous immuno¬ assays. See, for example, Ehrlich, P.H. et al.. Journal of Immunology 128;2709 (1982); ada, H.G. et al., Clin. Chem., ,2_i : __ 8δ2 (1982); Shimizu, S.Y. et al. , Clin. Che . , . 28:546 (1982); and Pettersson, K. et al., Clin. Chem., 29:60 (1983) . Serum-derived antibodies have also been em¬ ployed. See, for example, Sekiya, T. et al. , Acta Endo- crinologica 9_7: 562 (1981); Ayala, A.R. etal., J. Clinical Endocrinology and Medicine, £7:767 (1978) and ehmann, R.E. et al., Amer. J. of Obstetrics and Gynecology, 140:753 (1981). Immunoassays involving serum-derived an¬ tibodies have included antibodies directed to the unique carboxy terminal portion of the β subunit of hCG (ibid. ; and Birken, S. et al.. Endocrinology, 110:1555 [1982]). CMPI Although previous improvements in hCG immunoassays have provided increased sensitivity or specificity, or both, it has not been possible until the present invention to obtain the greatly enhanced sensitivity and almost ab- solute specificity achieved using the immunoassay dis¬ closed and claimed herein. This new immunoassay permits detection of smaller increases in hCG levels, and detec¬ tion much sooner after insemination, than previously possible. It also permits hCG measurements in cancer diagnosis unimpaired by significant hLH cross-reactions. Summary of the Invention This invention concerns an immunoassay for detecting hCG or measuring the level of hCG in a sample. The assay includes an antibody directed to the carboxy terminal portion of the 8 subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the 8 subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is detectable when both are bound to hCG. In a presently preferred embodiment the immunoassay can detect or measure levels of hCG or hCGβ in a urine sample and includes a detectable, purified, serum-derived anti¬ body directed to the carboxy terminal portion of the 3 subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the S subunit sufficiently remote from the carboxy terminal portion that both antibodies can simultaneously bind to hCG. The assay may be used to ' detect hCG or to measure hCG levels in urine samples. In this way the presence of hCG- producing neoplasms, subclinical spontaneous abortions and ectopic pregnancies can be diagnosed. OMPI A, IΪO Brief Description of the Drawings Fig. 1 is a standard curve showing use of the assay to measure hCG in buffer (© ©) and in urine (A Δ) and to show extent of cross-reactivity with h H in urine (El—B). Fig. 2 illustrates the sensitivity of a conventional radioimmunoassay (Con A Assay, Δ) as compared with the newly developed sandwich assay (0 O) n the measurement of hCG levels in an artificially inseminated female patient. Also depicted is the hypothetical pattern of hCG levels occurring as a result of subclinical spontaneous abortion ( ) . • OMPI , WIPO Detailed Description of the Invention Recent improvements in immunoassay technologies, par¬ ticularly the development of monoclonal antibodies, have permitted improvements in the sensitivity of assays for human chorionic gonadotropin (hCG). However, until the present invention assays have not been available which permit detection of increases in hCG levels a few days after insemination and which possess essentially absolute specificity for hCG, having no cross-reactivity with hLH. Specifically, this invention provides an immunoassay for detecting hCG or measuring levels of hCG, or both, in a sample. The immunoassay includes an antibody directed to the carboxy terminal portion of the β subunit of hCG which is a unique peptide sequence not found in hLH. The immunoassay also includes a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the β subunit that both antibodies can simultaneously bind to hCG. Finally, at least one of the antibodies is capable of detection when both antibodies are bound to hCG. Although in principle any antibody directed to the carboxy terminal portion of the β subunit of hCG would be effec¬ tive, the presently available and preferred antibodies are purified, serum-derived antibodies. Examples include R525 and R529 as described more fully hereinafter. How¬ ever, the present invention is not limited to serum- derived antibodies and also encompasses monoclonal anti¬ bodies directed to the carboxy terminal portion of the β subunit if and when efforts to produce such monoclonal antibodies are successful. OMPI Either antibody or both antibodies are capable of detec¬ tion when the antibodies are bound to hCG. Various means for rendering an antibody detectable are known to those skilled in the art. Merely by way of example, suitable means include radioactive labeling, e.g., 125j labeling, fluorescent labeling or linkage to an enzyme which cat¬ alyzes a detectable reaction, that is, an ELISA approach, e.g., linkage of an antibody to horseradish peroxidase. An additional approach involves the use of a third anti- body directed to one of the antibodies of the assay where the third antibody, either serum-derived or monoclonal, is detectable, e.g., labeledwith a radioactive isotope or a fluorescent moiety. The immunoassay of the present invention may be carried out totally in liquid phase, totally in solid phase or in a mixed liquid/solid system. Presently it is preferred that one of the antibodies, preferably the monoclonal antibody directed to a locus remote from the carboxy terminal portion of the β subunit be attached to a solid matrix, e.g., agarose or Sepharose. Although the immunoassay may be used with either urine, plasma, serum or other samples, it is particularly de- sirable to employ the immunoassay on urine samples because of the greater ease and convenience of obtaining such samples. This is, in fact, one of the substantial ad¬ vantages provided by the present invention since most presently available immunoassays for hCG are not suitable for use with urine samples. The presently preferred embodiment of the invention in¬ volves an immunoassay for detecting hCGβ or measuring levels of hCGβ, and thereby detecting or measuring hCG. This immunoassay includes a purified, serum-derived anti- body directed to the carboxy terminal portion of the β subunit of hCG and a monoclonal antibody directed to a locus on the βsubunit sufficiently remote from the carboxy terminal portion that both antibodies can simultaneously bind to hCG. In the preferred embodiment the purified, serum-derived antibody is detectable when both antibodies are bound to hCGβ, alone or as hCG, and the monoclonal antibody is attached to a solid matrix. This invention also concerns a purified antibody to the carboxy terminal portion of the β subunit of hCG, pref¬ erably labeled or otherwise detectable, e.g., i25ι_-ι a . beled. The immunoassay of this invention provides a method of detecting hCG or measuring the level of hCG, or both, in a urine sample. The sample to be tested is placed in the immunoassay under suitable conditions permitting forma¬ tion of a detectable or measurable complex with hCG. The resulting complex may then be detected if present or the level of hCG present determined by reference to a standard containing a known amount of hCG. * Measurements of hCG levels in turn can be used in the diagnosis or identification of disorders which involve production, or elevated levels, of hCG. Examples of such disorders include certain neoplasms, e.g., male testicu- lar cancer, subclinical spontaneous abortions and ectopic pregnancies. Methods for utilizing immunoassays for such purposes are well known to those skilled in the art and therefore are not described hereinafter in greater detail. The following section entitled "EXPERIMENTAL DETAILS" is set forth to aid in an understanding of the present invention but is not intended, and should not be con¬ strued, to limit the invention as defined by the claims which follow thereafter. EXPERIMENTAL DETAILS Materials and Methods Reagents The antibodies used in this experiment were R525 and R529, Birken, S. et al.. Endocrinology, 110:1555 (1982), rabbit antisera directed against the hCGβ- CTP determinants, and B101, Ehrlich, P.H. et al.. Journal of Immunology, 128:2709 (1982), a mouse monoclonal antibody directed against an hCGβ σonformational determinant. The methods of preparation and characteristics of these antibodies were as published in the preceding two cited references. The preparation- of highly purified hCG and hCG β subunit have been previously described. Canfield, R.E. and Morgan, F.J., in: Methods in Investigative and Diagnostic Endocrinology, edited by Berson, S.A. and Yalow, R.S., North-Holland, Amsterdam, p727 (1974). Human luteinizing hormone was a gift from the National Pituitary Agency (University of Maryland School of Medicine) NIAMDD, NIH. Concanavalin A covalently linked to agarose (Con A Sepharose), anhydrous α-methyl-D-mannoside and bovine γ- globulin, Cohn fraction II were purchased from Sigma Co. Cyanogen bromide (CnBr) activated Sepharose 4B was ob¬ tained from Pharmacia Fine Chemicals and DEAE Affi-Gel Blue from Bio-Rad Laboratories. The preceding purchased materials were used in accordance with the manufacturers' instructions. Monoclonal Antibody Production in Ascites Fluid, Purification and Coupling to Sepharose 4B The production of monoclonal anti-hCGβ was amplified OMPI utilizing the mouse ascites tumor system. Zola, H. and Brooks, D. in: Monoclonal Hybridoma Antibodies: Tech¬ niques and Applications, edited by J.G.R. Hurrell, CRC Press, Inc., Boca Raton, Florida, p50 (1982). One million B101 producing hybridoma cells were injected into each of thirty CD2F1 strain mice (West Seneca Laboratories) two weeks after they had been primed with one-half ml of pristane. Collection of ascites fluid commenced three weeks after injection of the cells and continued for two months. A total of 330 ml of ascites fluid was pooled and stored at -80°C. One aliquot of forty-six mis was dialysed extensively against 0.025 M Tris-HCl, pH 7.5, containing 0.05 M NaCl and 0.02% sodium azide at 4°C. The dialysate was applied to a 25 x 300 mm column of DEAE Affi-Gel Blue and eluted in five ml fractions with the same buffer used for dial¬ ysis. One hundred fractions containing the peak absorb- ance of 280 nm were pooled. The total amount of protein recovered in the preparation was calculated to be 1.46 g using the method of Warburg and Christian. Warburg, 0. and Christian, W., Biochem. Z., 310:384 (1941). Electro- phoresis of the preparation on nonreducing SDS poly- acryla ide gels, Weber, K. and Osborn, M. , J. Biol. Chem., 244:4406 (1969), indicated that at least 80% of the protein content was γ-globulin. Forty mis of the partially purified ascites preparation containing approximately 80 mg γ-globulin was dialysed against 0.1 M NaHCθ3' P H 8.0, containing 0.5 M NaCl at 4°C. The dialysate was then coupled to ten grams of CnBr activated Sepharose 4B according to the manufacturer's instructions. A 50% suspension (vol./vol.) of B101 cou¬ pled Sepharose 4B was prepared in 10 mM phosphate buffered saline, pH 7.4, containing 10 mM Na 2 EDTA, 0.1% sodium azide and 0.1% bovine γ-globulin (buffer B) and stored at 4°C. Purification of Rabbit Anti hCGβ-CTP (R525) and Iodination Ten mgs of hCGβ was coupled to two grams of CnBr activated Sepharose 4B. The hCGβ coupled Sepharose 4B was used to form an 0.9 x 10 cm column which was used for affinity purification of R525. A 54 ml pool of R525 from several different bleeds between boostings was recycled over the column continuously for 30 hours at 4°C using a peristaltic pump. The column was washed extensively with saline to remove loosely adhering material. The column was then eluted successively with 20 ml of 3M guanidine-HCl, pH 3.0, and 20 ml of 6 M guanidine-HCl, pH 3.0. The effluent was collected in one ml fractions and the absorbance at 280 nm determined. The absorbance profile had two discrete protein peaks corresponding in position to the areas of elution with 3 M and 6 M guanidine-HCl. The fractions having the peak absorbance at 280 nm were pooled and then dialysed extensively against 10 mM sodium acetate, pH 5.5, followed by 0.3 M aPθ4, pH 7.5, at 4°C. The dialysed preparations, an aliquot of the unpurified R525 pool and also the R525 pool which had been circulated over the h C Gβ affinity column were tested for their ability to bind 125 I-hCG. Only the unpurified R525 pool and the purifi¬ cation product eluted from the column with 6 M guanidine- HC1 had significant binding. A 1:1,500 dilution from a total volume of 5.7 ml of the purified R525 bound 50% of the trace as opposed to a 1.-600 dilution of the unpurified antiserum pool. The total protein content of the purified R525 was calculated to be 1.9 mg. Warburg, 0. and Christian, W. , Biochem. Z., 310:384 (1941). OMPI Thirty μg of the purifiedR525was radiolabeled with lmCiNa 125χ (A ersham) using Iodogen (Pierce Chemical Company) as the oxidizing agent. Fraker, P.J. and Speck, Jr. , J.D. , Biochem. Biophys. Research Comm., j30_:849 (1978). The specific activity of the iodinated product was approxi¬ mately 20 μCi/μg protein. Conduct of Sandwich Assay The conditions for the sandwich assay described below are the result of optimization by detailed analysis of reagent concentrations, incubation times and temperatures re¬ quired to give maximum hCG binding. Prior to assay, urine samples were adjusted to pH 7.4 with NaOH and centrifuged at 3000 x g for 15 minutes. Duplicate or triplicate four ml aliquots of urine, standards containing 0.004-0.5 mg hCG/ml in Buffer B, or Buffer B alone (for determination of nonspecific bindings; binding of trace [3- 2 5ι-R525] in the absence of hCG) were pipetted into 12 x 75 mm poly¬ styrene tubes. Two-tenths ml of a 6.25% suspension of B101 coupled Sepharose 4B in Buffer B, containing approxi¬ mately 25μg γ-globulin, was pipetted into each tube. The tubes were capped, placed horizontally on a Labquake Shaker (Labindustries) and incubated for two hours at room temperature with shaking in order to extract hCG from the samples. The tubes were centrifuged for 15 minutes at 3,000 x g. The supernatants were removed by aspiration, and the Bl01-Sepharose 4B pellets washed 2x with 2 ml Buffer B + 1% Tween 20. One-tenth ml of Buffer B containing 50,000 CPM 125 I-R525 was added to each tube. The samples were incubated vertically for 48-72 hours at 4°C with shaking on the Labquake Shaker. The tubes were then washed three times CMPI with 2 ml of Buffer B + 1% Tween 20 to reduce nonspecific binding. The radioactivity remaining after washing was determined in a Packard Auto-Gamma Scintillation Spectrometer. Data reduction for the generation of standard curves was ac¬ complished using a four parameter logistic fit. Rodbard, D. and Hutt, D.M., Proceedings Symposium on Radioimmuno- assay and Related Procedures in Medicine, International Atomic Energy Agency, Vienna, Austria, Unipub., New York, pl65 (1974). Conduct of the hCG RIA The hCG radioimmunoassay was conducted using the R529 antiserum to the hCGβ CTP determinants to measure hCG extracted from urine with σoncanavalin A as described by Wehmann,et al. Wehmann,R.E. , et al.. Clinical Chemistry, 27:1997 (1981). Patients First morning voided and random urines collected by normal volunteers at the Columbia Presbyterian Medical Center were used in order to establish the normal range of hCG excretion. The measurement of hCG for detection of early pregnancy was conducted using first morning voids pro- vided fay patients in the Department of Obstetrics and Gynecology, Columbia College of Physicians and Surgeons. These were normal women who were artificially inseminated because of the male partners' infertility. In order to correct for differences in the specific gravities of the various urines collected, urinary hCG concentration was normalized to creatinine concentration. Results Sandwich Assay Standard Curve A typical standard curve generated for hCG binding in the sandwich assay is shown in Fig. 1. The hCG dosages which give binding equivalent to 10% and 90% of Bmax (ΞDIQ and EDgo) have been tentatively accepted as the limits of the usable range of the standard curve. In the assay demon¬ strated EDio and EDgo correspond to 0.01 and 0.50 ng hCG/ml, giving a fiftyfold usable range. The Nonspecific Binding (NSB) in the assay is reduced to an acceptable level by extensive washing of the pellet with buffer containing Tween 20 and bovine γ-globulin. In the assay represented NSB was 2.5% of the total counts added. Fig. 1 also shows the dose response curve of hCG extracted from normal male urine which had been spiked with dosages identical to those used in the standards. The dose response curve for hCG in urine is essentially identical to that for hCG in Buffer B. The slope and ED50 of the dose response curve were 1.173 and 0.066 ng/ l for hCG in urine, compared to 1.098 and 0.072 ng/ml for hCG in buffer. The dose response curve for hLH added to normal male urine is also shown in Fig. 1. It should be noted that the units for hLH concentration are μg/ml as opposed to ng/ml for hCG concentration. A concentration of 2μg hLH/ l is required to obtain a dose response equivalent to 0.01 ng hCG/ml. Thus, the cross-reactivity of this concentration of hLH in the sandwich assay is 0.0005% on the basis of mass. This level of hLH is approximatelyfif y fold higher than those present in urine from women at the mid-cycle hLH surge. Saxena, B.B. et al.. Fertility and Sterility, 28:163 (1977). Therefore, physiologic concentrations of hLH are incapable of interfering in the sandwich assay. In summary. Fig. 1 shows that the assay is equally effec¬ tive for the measurement of hCG in buffer and in urine, highly sensitive, i.e., capable of detecting hCG at levels down to 0.004 ng/ml and that cross-reactivity with hLH is exceptionally low, i.e., about 0.0005%, thereby rendering the assay absolutely specific for hCG. Fig. 2 shows the use of the assay to measure hCG levels in the urine of an artificially inseminated female patient at various days after insemination. By way of comparison Fig. 2 also shows the use of a standard radioimmunoassay to measure hCG levels in the same patient's urine. As is clearly indicated in Fig. 2, the assay of the present invention is capable of detecting elevated hCG levels after the sixth day following insemination. By contrast, the standard RIA (sandwich assay) is less sensitive and cannot detect hCG until the tenth day following insemi¬ nation. Fig. 2 also illustrates the application of the assay in the diagnosis of subclinical spontaneous abortion. The hypo¬ thetical pattern illustrated in Fig. 2 shows that the standard RIA is incapable of detecting either the rise or the fall of hCG as the result of subclinical spontaneous abortion while the assay of the present invention will be able to do so because of its much greater sensitivity. OMPI -

Claims: WHAT IS CLAIMED IS: 1. An immunoassay for detecting and measuring hCG in a samplewhich comprises an antibody directed to the carboxy terminal portion of the β subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the β subunit of hCG that both antibodies can simul¬ taneously bind to hCG, wherein at least one of the anti- bodies is detectable when both are bound to hCG. 2. An immunoassay according to claim 1, wherein the antibody directed to the carboxy terminal portion of the β subunit of hCG is a purified, serum-derived antibody. 3. An immunoassay according to claim 1, wherein the antibody directed to the carboxy terminal portion of the β subunit of hCG is a monoclonal antibody. 4. An immunoassay according to claim 1, wherein the antibody directed to the carboxy terminal portion of the β subunit of hCG is detectable. ' 5. An immunoassay according to claim 4, wherein the detectable antibody is radioactively labeled. 6. An immunoassay according to claim 5, wherein the antibody is labeled with ^^- ^1. 7. An immunoassay according to claim 4, wherein the antibody is linked to an enzyme which catalyzes a detect¬ able reaction. 8. An immunoassay according to claim 7, wherein the enzyme is horseradish peroxidase. 9. An immunoassay according to claim -4, wherein the antibody is detectable upon formation of a complex with a third antibody which is labeled . 10. An immunoassay according to claim 2, wherein the antibody is R525 or R529. 11. An immunoassay according to claim 10, wherein the 525 or R529 is labeled with 12 5τ.. 12. An immunoassay according to claim 1, wherein the antibody directed to the carboxy terminal portion of the β subunit of hCG is attached to a solid matrix. 13. An immunoassay for hCGβ according to claim 1, wherein the monoclonal antinbody is directed to a determinant on the β subunit of hCG. 14. An immunoassay according to claim 13, wherein the monoclonal antibody is B101. 15. An immunoassay according to claim 1, wherein the monoclonal antibody directed to a remote determinant on hCG is detectable. 16. An immunoassay according to claim 1, wherein the monoclonal antibody directed to a remote determinant on hCG is attached to a solid matrix. 17. An immunoassay according to claim 16, wherein the matrix is agarose or Sepharose. 18. An immunoassay according to claim 1, wherein the sample is a urine sample. 19. An immunoassay for detecting and. measuring hCG or hCGβ in a urine sample which comprises a purified, serum-derived antibody directed to the carboxy terminal portion of the β subunit of hCG and a monoclonal antibody directed to a locus on the β subunit sufficiently remote from the carboxy terminal portion that both antibodies can simultaneously bind to hCG, wherein the purified, serum- derived antibody is detectable when both antibodies are bound to hCG or hCGβ and wherein the monoclonal antibody is attached to a solid matrix. 20. A purified antibody directed to the carboxy terminal portion of the β subunit of hCG. 21. A labeled, purified antibody according to claim 20. 22. 12 5ι_iabeled, purified R525. 23. 12 5ι_ia eled, purified R529. 24. A method of detecting hCG or measuring the level of hCG, or both, in a urine sample which comprises placing the sample in the immunoassay of claim 1 under suitable conditions permitting formation of a detectable or meas- urable complex with hCG and detecting or measuring the resulting complex. 25. A method of diagnosing for the presence of neoplasms producing hCG such as those associated with testicular cancer comprising measuring hCG levels according to the method of claim 24 and comparing the measured level with values associated with neoplasms. OMPΪ

Inventors: ARMSTRONG ELMO G, BIRKEN STEVEN, CANFIELD ROBERT E, EHRLICH PAUL H, ARMSTRONG, ELMO, G., BIRKEN, STEVEN, CANFIELD, ROBERT, E., EHRLICH, PAUL, H.
Applicants: UNIV COLUMBIA, THE TRUSTEES OF COLUMBIA UNIVERSITY
Application year: 1984
Main classification: G01N33
IPC codes: A61K39, C07K14, C07K16, C07K19, C12N15, C12Q1, G01N33