Chroman and chromene derivatives

Abstract: Compounds of formula (I): wherein: either one of R₁ and R₂ is hydrogen and the other is selected from the class of C₁₋₆ alkylcarbonyl, C₁₋₆ alkoxycarbonyl, C₁₋₆ alkylcarbonyloxy, C₁₋₆ alkylhydroxymethyl, nitro, cyano, chloro, trifluoromethyl, C₁₋₆ alkylsulphinyl, C₁₋₆ alkylsulphonyl, C₁₋₆ al­ koxysulphinyl, C₁₋₆ alkoxysulphonyl, C₁₋₆ alkylcarbonylamino, C₁₋₆ alkoxycarbonylamino, C₁₋₆ alkyl-thiocarbonyl, C₁₋₆ alkoxy­ thiocarbonyl, C₁₋₆ alkyl-thiocarbonyloxy, C₁₋₆ alkyl-thiomethyl, formyl or aminosulphinyl, aminosulphonyl or aminocarbonyl, the amino moiety being optionally substituted by one or two C₁₋₆ alkyl groups, or C₁₋₆ alkylsulphinylamino, C₁₋₆ alkylsul­ phonylamino, C₁₋₆ alkoxysulphinylamino or C₁₋₆ alkoxysul­ phonylamino or ethylenyl terminally substituted by C₁₋₆ alkyl­ carbonyl, nitro or cyano, or one of R₁ and R₂ is nitro, cyano or C₁₋₃ alkylcarbonyl and the other is methoxy or amino optionally substituted by one or two C₁₋₆ alkyl groups or by C₂₋₇ alkanoyl; one of R₃ and R₄ is hydrogen or C₁₋₄ alkyl and the other is C₁₋₄ alkyl or R₃ and R₄ together are C₂₋₅ polymethylene; either R₅ is hydrogen, hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆ is hydrogen or R₅ and R₆ together are a bond; R₇ is selected from the class consisting of C₁₋₆ alkyl substitut­ ed by amino optionally substituted by one or two C₁₋₆ alkyl groups which may be the same or different; amino optionally substituted by a C₁₋₆ alkyl or C₁₋₆ alkenyl group or a C₅₋₈ cyclo­ alkyl group or by a phenyl group optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; or C₁₋₆ alkoxy, or phenoxy op­ tionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; or, when X is oxygen, R₇ is selected from the class of carboxy, C₁₋₆ alkoxycarbonyl, or aminocarbonyl optionally substituted by one or two C₁₋₆ alkyl groups which may be the same or dif­ ferent; R₈ is hydrogen or C₁₋₆ alkyl; and X is oxygen or sulphur; The R₈-N-CX-R₇ group being trans to the R₅ group when R₅ and R₆ together are not a bond; or when the compound of formula (I) contains a salifiable group, pharmaceutically acceptable salts thereof, having phar­ macological activity, a process for preparing them, pharma­ ceutical compositions containing them, and their use in the treatment of mammals.

Description: The present invention relates to novel chromans and chromenes having pharmacological activity, to a process for preparing them, to pharmaceutical compositions containing them, and to their use in the treatment of mammals. US-A-4 110 347 and 4 119 643 and 4 251 537 and EP-A-28 064 and EP-A-28 449 disclose classes of chromans that are described as having blood pressure lowering activity or anti-hypertensive activity. European Patent Publications EP-A-76 075, EP-A-95 316, EP-A-35868, EP-A-28 065, EP-A-46 652 and EP-A-93 535 disclose further classses of chromans that are substituted in the 4-position. Such chromans are also described as having blood pressure lowering activity. J. Med. Chem. 26 (1983), No. 11, 1582-1589 describes the synthesis and anti-hypertensive activity of substituted trans -4-amino-2,2-dimethyl-2H-1-benzopyran-3-ols. A further class of chromans, and their corresponding chromenes, has now been discovered which are characterised by the presence of an ureido, thioureido or esterified carboxyamino or thiocarboxyamino group that substitutes the chroman or chromene in the 4-position. In addition, such chromans and chromenes have been found to have blood pressure lowering activity. Accordingly, the present invention provides a compound of formula (I): wherein: either one of R₁ and R₂ is hydrogen and the other is selected from the class of C₁₋₆ alkylcarbonyl, C₁₋₆ alkoxycarbonyl, C₁₋₆ alkylcarbonyloxy, C₁₋₆ alkylhydroxymethyl, nitro, cyano, chloro, trifluoromethyl, C₁₋₆ alkylsulphinyl, C₁₋₆ alkylsulphonyl, C₁₋₆ alkoxysulphinyl, C₁₋₆ alkoxysulphonyl, C₁₋₆ alkylcarbonylamino, C₁₋₆ alkoxycarbonylamino, C₁₋₆ alkyl-thiocarbonyl, C₁₋₆ alkoxy-thiocarbonyl, C₁₋₆ alkyl-thiocarbonyloxy, C₁₋₆ alkyl-thiolmethyl, formyl or aminosulphinyl, aminosulphonyl or aminocarbonyl, the amino moiety being optionally substituted by one or two C₁₋₆ alkyl groups, or C₁₋₆ alkylsulphinylamino, C₁₋₆ alkylsulphonylamino C₁₋₆ alkoxysulphinylamino or C₁₋₆ alkoxysulphonylamino or ethylenyl terminally substituted by C₁₋₆ alkyl-carbonyl, nitro or cyano, or one of R₁ and R₂ is nitro, cyano or C₁₋₃ alkylcarbonyl and the other is methoxy or amino optionally substituted by one or two C₁₋₆ alkyl groups or by C₂₋₇ alkanoyl; one of R₃ and R₄ is hydrogen or C₁₋₄ alkyl and the other is C₁₋₄ alkyl or R₃ and R₄ together are C₂₋₅ polymethylene; either R₅ is hydrogen, hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆ is hydrogen or R₅ and R₆ together are a bond; R₇ is amino optionally substituted by a C₁₋₆ alkyl or by a phenyl group optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; or C₁₋₆ alkoxy, or phenoxy optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen R₈ is hydrogen or C₁₋₆ alkyl; and X is oxygen or sulphur; the R₈-N-CX-R₇ group being trans to the R₅ group when R₅ and R₆ together are not a bond: or, when the compound of formula (I) contains a salifiable group, a pharmaceutically acceptable salt thereof. When one of R₁ and R₂ is hydrogen, the other is preferably selected from the class of C₁₋₆ alkylcarbonyl, C₁₋₆ alkoxycarbonyl, nitro or cyano. In particular, when one of R₁ and R₂ is hydrogen, the other is preferably nitro, cyano or acetyl. When one of R₁ and R₂ is hydrogen, it is preferred that R₂ is hydrogen. When one of R₁ and R₂ is nitro, cyano or C₁₋₃ alkylcarbonyl, and the other is methoxy or amino optionally substituted as hereinbefore defined, the other is preferably amino optionally substituted by one or two C₁₋₆ alkyl groups or by C₂₋₇ alkanoyl. In particular, when one of R₁ and R₂ is nitro, cyano or C₁₋₃ alkylcarbonyl, the other is amino, methylamino, dimethylamino or acetylamino. Most preferably, one of R₁ and R₂ is nitro or cyano, especially cyano, and the other is amino. When one of R₁ and R₂ is nitro, cyano or C₁₋₃ alkylcarbonyl, it is preferred that R₁ is nitro or cyano or C₁₋₃ alkylcarbonyl, such as acetyl. The alkyl groups or alkyl moieties of alkyl-containing groups for R₁ or R₂ are, preferably, methyl or ethyl. Preferably, R₃ and R₄ are both C₁₋₄ alkyl. In particular, they are both methyl or ethyl, preferably both methyl. When R₅ is C₁₋₆ alkoxy and R₆ is hydrogen, preferred examples of R₅ include methoxy and ethoxy, of which methoxy is more preferred. When R₅ is C₁₋₇ acyloxy and R₆ is hydrogen, a preferred class of R₅ is unsubstituted carboxylic acyloxy, such as unsubstituted aliphatic acyloxy. However, it is preferred that R₅ and R₆ together are a bond, or, in particular, that R₅ is hydroxy and R₆ is hydrogen. Examples of R₇ include the following groups: Amino optionally substituted by a methyl, ethyl, propyl, butyl, or trichloroacetyl group or by a phenyl group optionally substituted by one methyl, methoxy or chloro group or atom, in particular amino, methylamino, and phenylamino optionally substituted in the phenyl ring by one methyl, methoxy or chloro group or atom. Methoxy, ethoxy, n- and iso-propoxy, and phenoxy optionally substituted by one methyl, ethyl, methoxy, ethoxy, chloro or bromo group or atom, preferably methoxy, ethoxy, and phenoxy optionally substituted by one methyl, methoxy or chloro group or atom, in particular ethoxy X is preferably oxygen in this case. Examples of R₈ include hydrogen, methyl, ethyl, n -or iso -propyl. Preferably, R₈ is hydrogen or methyl, especially hydrogen. Examples of a pharmaceutically acceptable salt of a compound of formula (I), when the compound of formula (I) contains a salifiable group which is an optionally substituted amino group, include acid addition salts such as the hydrochloride and hydrobromide salts. Such a group may be an R₁ or R₂ group or be within an R₇ group. Preferably, a compound of formula (I) is in substantially pure form. The compounds of formula (I) wherein R₅ is hydrogen, hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆ is hydrogen have a least one asymmetric centre and, therefore, can exist as enantiomers. The present invention extends to all such isomers individually and as racemates. Examples of compounds of formula (I) include the compounds prepared in the Examples hereinafter. A group of compounds of formula (I) is those wherein R₇ is amino optionally substituted by a C₁₋₆ alkyl group, or by a phenyl group optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen. A second group of compounds of formula (I) is those wherein R₇ is C₁₋₆ alkoxy, or phenoxy optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen. The present invention also provides a process for preparing a compound of formula (I), which comprises acylating a compound of formula (II): wherein R₁' and R₂' are R₁ and R₂, as defined hereinbefore, or a group or atom convertible thereto, R₃, R₄ and R₈ are as defined hereinbefore, R₅' is hydrogen, hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆' is hydrogen, the R₈NH group being trans to the R₅' group with a compound of formula (IV) X=C=N.R₁₁ (IV) wherein R₁₁ is hydrogen, C₁₋₆ alkyl or phenyl optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; and X is oxygen or sulphur, and thereafter when R₁₁ is hydrogen, optionally converting R₁₁ to other R₁₁; and thereafter in the case where R₁' or R₂' is a group or atom convertible into R₁ or R₂, converting the group or atom into R₁ or R₂; optionally converting R₁, R₂ or R₅ in the resulting compound of formula (I) into another R₁, R₂ or R₅; in the case where R₅ and R₆ in the resulting compound are hydroxy and hydrogen respectively, optionally dehydrating the compound to give a compound wherein R₅ and R₆ together are a bond; in the case where R₅ and R₆ together are a bond in the resulting compound, optionally reducing the compound to give a compound wherein R₅ and R₆ are each hydrogen; and optionally thiating the carbonyl group of any R₈NCOR₇ group in the resulting compound of formula (I) to give another compound of formula (I), wherein X is sulphur; and, when the resulting compound of formula (I) containing a salifiable group, optionally forming a pharmaceutically acceptable salt thereof. In the process, when R₁₁ in a compound of formula (IV) is C₁₋₆ alkyl or phenyl optionally substituted as hereinbefore defined, the reaction between the compounds of formulae (II) and (IV) is, preferably, carried out in a solvent, such as methylene chloride, at below room temperature, in particular below 10 o C. When R₁₁ is hydrogen, the reaction between the compounds of formulae (II) and (IV) is, preferably, carried out using a corresponding alkali metal cyanate or thiocyanate, for example that of sodium or potassium, in an aqueous medium(optionally methanolic) acidified with a mineral acid, such as dilute hydrochloric acid. A slightly elevated temperature such as 50 to 90 o C is apt. Conversions of an aromatic group into R₁ or R₂, as defined hereinbefore, are generally known in the art of aromatic chemistry. For example, it is preferred when carrying out the acylation of a compound of formula (II), first to protect any unsubstituted amino group, that may be present for R₁ or R₂, and afterwards to convert the protected amino moiety into the required amino group. Examples of protecting agents include acyl groups, such as acetyl, which may be added and removed conventionally. If it is desired to protect an amino moiety in the presence of a cyano group then a more appropriate method is to use a trifluoroacetyl protecting group which may be removed by mild hydrolysis or to use a benzyloxycarbonyl or a p-nitrobenzyloxycarbonyl protecting group which may be removed by mild catalytic hydrogenolysis. If the optional thiation reaction is to be carried out in order to obtain a compound of formula (I), wherein one or the other of R₁ and R₂ is a carbonyl-containing group and X is sulphur, it is preferred to use in the acylation reaction the corresponding compound of formula (II), wherein R₁' or R₂' is a protected carbonyl-containing group, and after thiation to convert the protected carbonyl-containing group into the required carbonyl-containing group for R₁ or R₂. Without such protection, the additional carbonyl group may give rise to a competing side-reaction. Examples of preferred carbonyl protecting groups include ketalising agents, which may be added and removed in conventional manner. Examples of an optional conversion of R₁ or R₂ in the resulting compound of formula (I) into another R₁ or R₂, as defined hereinbefore, include the optional conversion of an α-hydroxyethyl group into acetyl by oxidation, the optional conversion of an amino group into a chlorine atom by the Sandmeyer reaction the optional conversion of an amino group into an amino group substituted by one or two C₁₋₆ alkyl or by C₂₋₇ alkanoyl, or the optional conversion of a hydrogen atom into a nitro group by nitration. Examples of an optional conversion of R₅ in a compound of formula (I) into another R₅ are generally known in the art. For example, when R₅ is hydroxy, it may be alkylated using an alkyl iodide in an inert solvent, such as toluene, in the presence of a base, such as potassium hydroxide, or it may be acylated using a carboxylic acid chloride or anhydride in a non-hydroxylic solvent in the presence of a base such such as triethylamine. Alternatively, when R₅ is C₂₋₇ acyloxy or it may be converted into hydroxy by conventional hydrolysis with for example, dilute mineral acid. The optional dehydration of a resulting compound of formula (I), wherein R₅ and R₆ are hydroxy and hydrogen respectively, into another compound of formula (I), wherein R₅ and R₆ together are a bond, may be carried out in accordance with conventional dehydration conditions, for example, by using a dehydrating agent, such as sodium hydride, in an inert solvent, such as dry tetrahydrofuran, at reflux temperature. The optional reduction of an R₅-R₆ bond may be effected by conventional catalytic hydrogenation for example using palladium on charcoal. groups which may be the same or different. The optional thiation of the R₈-N-CO-R₇ group in a compound of formula (I) to give another compound of formula (I), wherein X is sulphur, is, preferably, carried out with conventional thiation agents, such as hydrogen sulphide, phosporous pentasulphide and Lawesson's reagent (p-methoxyphenylthiophosphine sulphide dimer). The use of hydrogen sulphide or phosporous pentasulphide may lead to side-reactions and, therefore, the use of Lawesson's reagent is preferred. The thiation reaction conditions are conventional for the thiation agent employed. For example, the use of hydrogen sulphide is, preferably, acid catalysed by, for example, hydrogen chloride in a polar solvent, such as acetic acid or ethanol. The preferred use of Lawesson's reagent is preferably carried out under reflux in a dry solvent, such as toluene or methylene chloride. The optional formation of a pharmaceutically acceptable salt, when the resulting compound of formula (I) contains a salifiable group may be carried out conventionally. A compound of formula (II) may be prepared by reacting a compound of formula (V): wherein R₁', R₂', R₃ and R₄ are as defined hereinbefore, with a compound of formula (VI): R₈NH₂ (VI) wherein R₈ is as defined hereinbefore; and optionally converting the hydroxy group for R₅' in the resulting compound of formula (II) into a C₁₋₆ alkoxy or C₁₋₇ acyloxy group. The reaction is normally carried out in a solvent, such as a C₁₋₄ alcohol, in particular methanol, ethanol or propanol at an ambient or an elevated temperature, for example 12 to 100 o C. The reaction proceeds particularly smoothly if carried out in ethanol under reflux. The resulting compound of formula (II) may be removed from the reaction mixture by removal of the solvent, for example, by evaporation under reduced pressure. Any epoxide impurity may be removed conventionally, for example by chromatography. The optional conversion of the hydroxy group for R₅' in the resulting compound of formula (II) into a C₁₋₆ alkoxy or C₁₋₇ acyloxy group may be carried out as described hereinbefore in relation to the corresponding conversion of R₅ in a compound of formula (I). A compound of formula (VI) may be prepared, preferably in situ , by reacting a compound of formula (VII): wherein R₁', R₂', R₃ and R₄ are as defined hereinbefore and the hydroxy group is trans to the bromo atom, with a base, such as potassium hydroxide, in a solvent, such as ether or aqueous dioxan. Compounds of formula (VII) are known and may be prepared in accordance with any appropriate known process, for example, by the process described in the aforementioned U.S. patents and European patent publications. Schematically, such process can be depicted thus. (a) Room temperature; NaOH/40% benzyltrimethylammonium hydroxide in methanol; (b) Heat in o-dichlorobenzene; (c) N-bromosuccinimide/dimethylsulphoxide/water; (d) Bromine in carbon tetrachloride; and (e) Acetone/water. The above process may produce mixtures of compounds during reaction (b) owing to the two sites available for ring formation. It is therefore advisable to remove any of the undesired compound by, for example, chromatography, before reaction (c) or (d). As mentioned previously, the compounds of formula (I) exist in optically active forms, and the processes of the present invention produce mixtures of such forms. The individual isomers may be separated one from the other by chromatography using a chiral phase. It is preferred that the compounds of formula (I) are isolated in substantially pure form. The compounds of formula (V) to (VII) are also known or can be prepared analogously to the preparation of known compounds, or are routinely derivable from known compounds. As mentioned previously, the compounds of formula (I) have been found to have blood-pressure lowering activity. They are therefore useful in the treatment of hypertension. The present invention accordingly provides a pharmaceutical composition which comprises a compound of formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier. In particular, the present invention provides an anti-hypertensive pharmaceutical composition which comprises an anti-hypertensive effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier. The compositions are preferably adapted for oral administration. However, they may be adapted for other modes of administration, for example parenteral administration for patients suffering from heart failure. The compositions may be in the form of tablets, capsules, powders, granules, lozenges, suppositories, reconstitutable powders, or liquid preparations, such as oral or sterile parenteral solutions or suspensions. In order to obtain consistency of administration it is preferred that a composition of the invention is in the form of a unit dose. Unit dose presentation forms for oral administration may be tablets and capsules and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrrolidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate; disintegrants, for example starch, polyvinylpyrrolidone, sodium starch glycollate or microcrystalline cellulose; or pharmaceutically acceptable wetting agents such as sodium lauryl sulphate. The solid oral compositions may be prepared by conventional methods of blending, filling, tabletting or the like. Repeated blending operations may be used to distribute the active agent throughout those compositions employing large quantities of fillers. Such operations are of course conventional in the art. The tablets may be coated according to methods well known in normal pharmaceutical practice, in particular with an enteric coating. Oral liquid preparations may be in the form of, for example, emulsions, syrups, or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methyl cellulose, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminium stearate gel,hydrogenated edible fats; emulsifying agents, for example lecithin, sorbitan moncoleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, fractionated coconut oil, oily esters such as esters of glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid; and if desired conventional flavouring or colouring agents. For parenteral administration, fluid unit dosage forms are prepared utilizing the compound and a sterile vehicle, and, depending on the concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved in water for injection and filter sterilized before filling into a suitable vial or ampoule and sealing. Advantageously, adjuvants such as a local anaesthetic, a preservative and buffering agents can be dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. Parenteral suspensions are prepared in substantially the same manner, except that the compound is suspended in the vehicle instead of being dissolved, and sterilization cannot be accomplished by filtration. The compound can be sterilized by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound. The compositions may contain from 0.1% to 99% by weight, preferably from 10-60% by weight, of the active material, depending on the method of administration. An effective amount will depend on the relative efficacy of the compounds of the present invention, the severity of the hypertension being treated and the weight of the sufferer. However, a unit dose form of a composition of the invention may contain from 1 to 100 mg of a compound of the invention and more usually from 2 to 50 mg, for example 5 to 25 mg such as 6, 10, 15 or 20mg. Such compositions may be administered from 1 to 6 times a day, more usually from 2 to 4 times a day, in a manner such that the daily dose is from 5 to 200 mg for a 70 kg human adult and more particularly from 10 to 100 mg. The present invention further provides a compound of formula (I) or a pharmaceutically acceptable salt thereof for use in the treatment or prophylaxis of maladies in mammals, in particular of hypertension. The following examples relate to the preparation of compounds of formula (I). All temperatures therein are in o C. Example 1 Trans-6-Cyano-3,4-dihydro-2,2-dimethyl-4-(N-methylureido)-2H-1-benzopyran-3-ol (E2) Trans-4-amino-6-cyano-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-3-ol (2.00g) in dichloromethane (30ml) was added dropwise to a stirred solution of methyl isocyanate (0.54ml) in dichloromethane (15ml), the temperature being maintained at below 10 o C. After 15 min a white precipitate formed and the reaction mixture was allowed to reach room temperature. The solid was filtered and recrystallised from dichloromethane to give the title compound (1.30g) as crystals of m.p. 143-146 o C. NMR (DCDl₃) δ 1.25 (3H,s) 1.46 (3H,s) 2.73 (3H,d,J=5Hz) collapsing to singlet on addition of D₂O 3.56 (1H,d,J=9Hz) 4.75 (1H,t,J=9Hz) collapsing to doublet J=9 on addition of D₂O 5.01 (1H,s,exchangeable) 5.40-5.75 (2H,m,exchangeable) 6.85 (1H,d,J=8Hz) 7.40 (1H,q,J=8,2Hz) 7.15 (1H,irreg s) The starting material was prepared in accordance with the procedure described in European Patent Publication 76 075. Example 2 6-Cyano-3,4-dihydro-2,2-dimethyl-trans-4-(N-phenylureido)-2H-1-benzopyran-3-ol (E3) To trans-4-amino-3,4-dihydro-2,2-dimethyl-2H-benzo[b]-pyran-3-ol (2.0g) dissolved in ice cooled dichloromethane (25ml), was added phenylisocyanate (1ml) in dichloromethane (15ml). The title compound, a white solid, precipitated out, and this was collected by filtration, and dried (2.6g). m.p. 226-227 o C. NMR (CDCl₃/CD₃OD) δ 1.30 (3H,s) 1.50 (3H,s) 3.60 (1H,d,J=10Hz) 4.88 (1H,d,J=10Hz) 6.87 (1H,d,J=9Hz) 6.97-7.75 (7H, series of m). Example 3 Trans-4-(N-butylureido)-6-cyano-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-3-ol (E4) To trans-4-amino-6-cyan-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-3-ol (1.00g) in dichloromethane (40ml) cooled in an ice bath was added butylisocyanate (0.25 ml)in dichloromethane. The solution was stirred for 30 min and a white precipitate formed. This precipitate was collected and dried (1.12g) and identified as the title compound; mp 173-174 o C. NMR (CDCl₃) δ 1.00 (3H,irreg d,J-8Hz) 1.27 (3H,s) and 1.50 (3H,s) overlapping 1.25-1.65 (4H,m) 3.20 (2H,irreg t,J=8,8Hz) 3.57 (1H,d,J=9Hz) 4.70-5.30 (3H,m) collapsing to 4.83 (1H,d,J= 9Hz) on addition of D₂O 6.87 (1H,d,J=9Hz) 7.43 (1H,q,J=9,2Hz) 7.68 (1H,narrow m) Trans-6-cyano-3,4-dihydro-2,2-dimethyl-4-(N-n-propylureido)-2H-1-benzopyran (E14) is prepared in an analogous manner. Example 4 Trans-6-cyano-3,4-dihydro-2,2-dimethyl-4-(N-methylthiureido)-2H-1-benzopyran-3-ol (E6) Methyl isothiocyanate (0.34g) in dichloromethane (10ml) was added to trans-4-amino-6-cyano-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-3-ol (1.00g) stirred in dichloromethane (30ml) at below 10 o C. The reaction was stirred for 66 hours at room temperature. The solid which precipitated out was filtered (0.7g) and identified as the title thiureide mp 203-205 o C. Mass spectrum M⁺-H₂O at M/Z 273.0934 Calcd for C₁₄H₁₅N₃OS 273.0936 Example 5 Trans-6-acetyl-3,4-dihydro-2,2-dimethyl-4-(N-methylthiureido)-2H-1-benzopyran-3-ol (E8) The compound of example 8 was prepared in an analogous manner to the compound of (E6), employing trans-6-acetyl-4-amino-3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-3-ol as starting material. The title compound was recrystallised from ethyl acetate-pentane, mp 203-204 o C. NMR (CDCl₃ + CD₃OD) δ 1.34 (3H,s) 1.51 (3H,s) 2.06 (3H,s) 3.10 (3H,s) 3.66 (1H,d,J=9Hz) 5.67 (1H,d,J=9Hz) 6.86 (1H,d,J=9Hz) 7.80 (1H,q,J=9,2Hz) 7.94 (1H,narrow m) Example 6 Trans-6-cyano-3,4-dihydro-2,2-dimethyl-4-(N-ureido)-2H-1-benzopyran-3-ol (E11) To a stirred suspension of 6-cyano-3,4-dihydro-epoxy-2H-benzo[b]pyran (1.0g) in water (30 mls) at 0 o C was added 5N hydrochloric acid (0.92 mls). After warming to 70 o C, methanol (3 mls) was added to give a homogeneous solution and sodium cyanate (0.3g) added. After 2½ hours the reaction was allowed to cool and crystallise. The resulting solid was filtered off, chromatographed, and recrystallised to give the title compound as crystals of m.p. 212-214 o C. NMR (CO₃OD) δ 1.29 (3H, s) 1.51 (3H, s) 3.55 (1H, d, J=9Hz) 4.79 (1H, d, J=9Hz) 6.88 (1H, d, J=9Hz) 7.48 (1H, q, J=9,2Hz) 7.70 (1H, narrow m) Pharmacological Data - Antihypertensive Activity Systolic blood pressures were recorded by a modification of the tail cuff method described by I.M.Claxton, M.G. Palfreyman, R.H. Poyser, R.L. Whiting, European Journal of Pharmacology, 37 , 179 (1976). W+W BP recorder, model 8005, was used to display pulses. Prior to all measurements rats were placed in a heated environment (33.5±0.5°C) before transfer to a restraining cage. Each determination of blood pressure was the mean of at least 6 readings. Spontaneously hypertensive rats (ages 12-18 weeks) with systolic blood pressures >170 mmHg were considered hypertensive. Compound (E2) Time Post Dose Hours % Change in Systolic Blood % Change in Heart Rate 6 rats Dose 1mg/kg 1 -51 ± 5 8 ± 6 p.o. 2 -57 ± 3 7 ± 2 Initial Blood Pressure 4 -61 ± 3 12 ± 3 256± 4 mmHg 6 -52 ± 4 11 ± 3 Initial Heart Rate 441± 9 beats/min Compound (E6) Time Post Dose Hours % Change in Systolic Blood Blood % Change in Heart Rate 6 rats Dose 3 mg/kg 1 -31 ± 2 6 ± 3 p.o. 2 -33 ± 3 -2 ± 2 Initial Blood Pressure 4 -22 4 212 ± 5 mmHg 6 -13 ± 6 -7 ± 2 Initial Heart Rate 503 ± 11 beats/min Compound (E9) Time Post Dose Hours % Change in Systolic Blood Blood % Change in Heart Rate 6 rats Dose 10 mg/kg 1 -22 ± 3 -1 ± 2 p.o. 2 -28 ± 2 -1 ± 3 Initial Blood Pressure 4 -26 ± 2 -5 ± 3 213 ± 7 mmHg 6 -15 ± 2 -3 ± 3 Initial Heart Rate 485 ± 7 beats/min Compound (E10) Time Post Dose Hours % Change in Systolic Blood Blood % Change in Heart Rate 6 rats Dose 10 mg/kg 1 -54 ± 3 -3 ± 4 p.o. 2 -40 ± 3 -1 ± 3 Initial Blood Pressure 4 -33 ± 0 -8 ± 3 245 ± 5 mmHg 6 -30 ± 4 -11 ± 3 Initial Heart Rate 454 ± 15 beats/min

Claims: A compound of formula (I): wherein: either one of R₁ and R₂ is hydrogen and the other is selected from the class of C₁₋₆ alkylcarbonyl, C₁₋₆ alkoxycarbonyl, C₁₋₆ alkylcarbonyloxy, C₁₋₆ alkylhydroxymethyl, nitro, cyano, chloro, trifluoromethyl, C₁₋₆ alkylsulphinyl, C₁₋₆ alkylsulphonyl, C₁₋₆ alkoxysulphinyl, C₁₋₆ alkoxysulphonyl, C₁₋₆ alkylcarbonylamino, C₁₋₆ alkoxycarbonylamino, C₁₋₆ alkyl-thiocarbonyl, C₁₋₆ alkoxy-thiocarbonyl, C₁₋₆ alkyl-thiocarbonyloxy, C₁₋₆ alkyl-thiolmethyl, formyl or aminosulphinyl, aminosulphonyl or aminocarbonyl, the amino moiety being optionally substituted by one or two C₁₋₆ alkyl groups, or C₁₋₆ alkylsulphinylamino, C₁₋₆ alkylsulphonylamino C₁₋₆ alkoxysulphinylamino or C₁₋₆ alkoxysulphonylamino or ethylenyl terminally substituted by C₁₋₆ alkyl-carbonyl, nitro or cyano, or one of R₁ and R₂ is nitro, cyano or C₁₋₃ alkylcarbonyl and the other is methoxy or amino optionally substituted by one or two C₁₋₆ alkyl groups or by C₂₋₇ alkanoyl; one of R₃ and R₄ is hydrogen or C₁₋₄ alkyl and the other is C₁₋₄ alkyl or R₃ and R₄ together are C₂₋₅ polymethylene; either R₅ is hydrogen, hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆ is hydrogen or R₅ and R₆ together are a bond; R₇ is amino optionally substituted by a C₁₋₆ alkyl group or by a phenyl group optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; or C₁₋₆ alkoxy, or phenoxy optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; R₈ is hydrogen or C₁₋₆ alkyl; and X is oxygen or sulphur; the R₈-N-CX-R₇ group being trans to the R₅ group when R₅ and R₆ together are not a bond; or, when the compound of formula (I) contains a salifiable group, a pharmaceutically acceptable salt thereof. A compound according to claim 1 wherein R₅ is hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆ is hydrogen or R₅ and R₆ together are a bond. A compound to claim 1 or 2, wherein R₁ is nitro, cyano or acetyl, and R₂ is hydrogen. trans -6-Cyano-3,4-dihydro-2,2-dimethyl-4-(N-methylureido)-2H-1-benzopyran-3-ol. tran s-6-Cyano-3,4-dihydro-2,2-dimethyl-4-(N-phenylureido)-2H-1-benzopyran-3-ol. A process for preparing a compound according to any one of claims 1 to 5, which comprises acylating a compound of formula (II): wherein R₁' and R₂' are R₁ and R₂, as defined in claim 1, or a group or atom convertible thereto, R₃, R₄ and R₈ are as defined in claim 1, R₅' is hydrogen, hydroxy, C₁₋₆ alkoxy or C₁₋₇ acyloxy and R₆' is hydrogen, the R₈NH group being trans to the R₅' group, with a compound of formula (IV): X=C=N.R₁₁ (IV) wherein R₁₁ is hydrogen, C₁₋₆ alkyl or phenyl optionally substituted by C₁₋₆ alkyl, C₁₋₆ alkoxy or halogen; and X is oxygen or sulphur; and thereafter when R₁₁ is hydrogen, optionally converting R₁₁ to other R₁₁; and thereafter, in the case where R₁' or R₂' is a group or atom convertible into R₁ or R₂ as defined in claim 1, converting the group or atom into R₁ or R₂; optionally converting R₁, R₂ or R₅ as defined in claim 1 in the resulting compound of formula (I) into another R₁, R₂ or R₅; in the case where R₅ and R₆ in the resulting compound of formula (I) are hydroxy and hydrogen respectively, optionally dehydrating the compound to give a compound of formula (I) wherein R₅ and R₆ together are a bond; in the case where R₅ and R₆ together are a bond in the resulting compound, optionally reducing the compound to give a compound wherein R₅ and R₆ are each hydrogen; and optionally thiating the carbonyl group of any R₈NCOR₇ group in the resulting compound of formula (I) to give another compound of formula (I), wherein X is sulphur; and, when the resulting compound of formula (I) contains a salifiable group, optionally forming a pharmaceutically acceptable salt thereof. A pharmaceutical composition which comprises a compound according to any one of claims 1 to 5 and a pharmaceutically acceptable carrier. A compound according to any one of claims 1 to 5, for use as an active therapeutic substance. A compound according to any one of claims 1 to 5, for use in the treatment or prophylaxis of hypertension in mammals. Use of a compound according to any one of claims 1 to 5, in the manufacture of a medicament for use in the treatment of hypertension in mammals.

Inventors: CASSIDY FREDERICK, EVANS JOHN MORRIS, CASSIDY, FREDERICK, EVANS, JOHN MORRIS
Applicants: BEECHAM GROUP PLC
Application year: 1984
Main classification: C07D311
IPC codes: A61K31, C07D311, A61P9