DERIVATIVES OF BETA-AMINO-GAMMA-TRIMETHYLAMMONIO-BUTYRATE, THEIR PRODUCTION AND USE

Abstract: A compound of the formula: wherein R₁ is hydrogen, alkyl or acyl, R₂ is hydroxy, alkoxy or amino, is useful as an antidiabetic agent.

Description: DERIVATIVES OF BETA-S > IINO-GASSE-TRItETHYII2QNIONIO-BUTYRATE AND THEIR PRODUCTION AND USE This invention relates to derivatives of ss-amino-y- trimethylammonio-butyrate and their production and use. There has for sometime been a demand for a more effective antidiabetic drug having a new mode of action for the treatment of diabetes and its complications, the incidence of which has been on the steady increase. Thus, in diabetes, as an insulin deficiency promotes a liberation of fatty acids in the adipose tissue, there occurs an increased supply of fatty acids to the liver and as the decomposition of fatty acids is concurrently promoted, the production of ketone compounds is stimulated, leading to the so-called ketonemia. In the extrahepatic tissues, the utilization of glucose is poor and the ketone compounds so produced are used as energy sources. Therefore, it is expected that if the decomposition of fatty acids is inhibited, the production of ketone compounds will be attenuated and, consequently, the utilization of glucose be promoted so that blood sugar levels are ultimately decreased. In other words, a specific inhibitor of fatty acid degradation would be of value as a new antidiabetic drug relying on a new mechanism of action. In view of these background facts, the present inventors made an extensive study for the development of a new antidiabetic and discovered that derivatives of the compound of the formula: EMI2.1 are substances capable of inhibiting the decomposition of fatty acids, and have completed the present invention. The present invention is directed to: (1) a compound of the formula: EMI2.2 wherein R1 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue and R2 is a group represented by the formula -OR3 (in which R3 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue) or by the formula -NH-R4 (in which R4 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue), provided that when R1 is a hydrogen atom or an acetyl group, R2 is not a hydroxy group; or a pharmaceutically acceptable salt thereof; (2) a method of producing the compound (I) or a salt thereof, which comprises subjecting a compound of the formula: EMI2.3 or a salt thereof to hydrocarbon residue introduction reaction, esterification reaction and/ or amidation reaction; (3) an antidiabetic agent which contains an antidiabetically effective amount of the compound (I) or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier, excipient or diluent therefor, (4) a compound in the optically active D-form which is represented by the formula: EMI3.1 wherein R5 is a hydrogen atom or an acetyl group; or a salt thereof; and (5) a method of producing a compound of the formula: EMI3.2 wherein R6 is -COCH2CH3 or -COCH2CH2CH3, which comprises cultivating a microorganism belonging to the genus Emericella and capable of producing the compound (IV) in a culture medium so as to cause formation and accumulation of the compound (IV) in the culture medium and isolating said compound. In the present specification, the compound of the formula (I) in which R1 is acetyl and R2 is hydroxy, when in the optically active L-form, is sometimes referred to as "FA-585911. The compound of the formula (I) in which R1 is hydrogen and R2 is hydroxy is ss-amino-y-trimethyl- ammonio-butyrate, and when in the optically active L-form it is sometimes referred to as "deacetyl FA-5859". In the above formulas, the hydrocarbon residue which is represented by any of R1, R3 and R4 and may optionally be substituted may be any of carbon-containing groups and the carbon atom of which is available for bonding. More specifically, said hydrocarbon residue includes, among others, alkyl, alkenyl, aryl, aralkyl and heterocyclic groups. Said alkyl group preferably contains 1 to 20 carbon atoms. Examples are methyl, ethyl, n-propyl, isopropyl, cyclopropyl, n-butyl, isobutyl, tert-butyl, cyclobutyl, pentyl, isopentyl, cyclopentyl, hexyl, isohexyl, cyclohexyl, heptyl, cycloheptyl, octyl, cyclooctyl, nonyl, decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl and eicosyl. The above-mentioned alkenyl preferably contains 1 to 18 carbon atoms and is, for example, vinyl, propenyl, allyl, butenyl or oleyl. The above-mentioned aryl is, for example, phenyl or naphthyl. The above-mentioned aralkyl is, for example, benzyl, a-methylbenzyl, phenethyl, diphenylmethyl, l-phenylpropyl or l-phenylbutyl. The above-mentioned heterocyclic group is, for example, pyridyl, pyrazinyl, pyrrolidinyl or piperidyl. The alkyl group mentioned above as a hydrocarbon residue may be substituted, for instance, by lower alkoxy, oxo, halogen, hydroxy, nitro, amino, cyano, sulfo, aryl and/or aralkyloxy. The alkenyl group mentioned above as a hydrocarbon residue may be substitued, for instance, by lower alkoxy, oxo, halogen, hydroxy, aryl. The aryl, aralkyl and heterocyclic groups each mentioned above as a hydrocarbon residue may be substitued, for example, by lower alkyl, lower alkoxy, oxo, halogen, hydroxy, nitro, amino, cyano, sulfo and/or phenyl. The lower alkyl mentioned above as a substituent preferably contains 1 to 6 carbon atoms and includes, among others, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-pentyl, isopentyl, cyclopentyl, hexyl, isohexyl and cyclohexyl. The lower alkoxy mentioned above as a substituent preferably contains 1 to 6 carbon atoms. Examples are methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, secbutoxy, tert-butoxy, n-pentyloxy, isopentyloxy and nhexyloxy, and more preferably those containing 1 to 4 carbon atoms. The above-mentioned halogen as a substituent is, for example, chlorine, bromine, iodine or fluorine. The aryl mentioned above as a substituent is, for example, phenyl. The aralkyloxy mentioned above as a substituent is, for example, phenyl-Cl 4 alkyloxy. The C14 alkyl in the above-mentioned phenyl-Cl 4 alkyloxy is, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl or tert-butyl. As the aralkyloxy, benzyloxy is more. preferable. Preferable examples of the hydrocarbon residue in the substituted or unsubstituted hydrocarbon residue of R are alkyl of 1 to 6 carbon atoms and acyl of the formula -CO-R7 (wherein R7 is hydrogen or alkyl of 1 to 17 carbon atoms). Examples of the above-mentioned alkyl of 1 to 6 carbon atoms are methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, n-pentyl, isopentyl, n-hexyl and isohexyl. Examples of the above-mentioned C1-17 alkyl in the symbol R7 are methyl, ethyl, n-propyl, isopropyl, cyclopropyl, n-butyl, isobutyl, tert-butyl, cyclobutyl, pentyl, isopentyl, cyclopentyl, hexyl, isohexyl, cyclohexyl, heptyl, cycloheptyl, octyl, cyclooctyl, nonyl, decyl, undecyl, dodecyl, tridecyl, tetradecyl r pentadecyl, hexadecyl and heptadecyl. The above-mentioned alkyl of 1 to 6 carbon atoms and acyl group of the formula -CO-R7 may be substitued by lower alkoxy, halogen, hydroxy, nitro, amino, cyano, sulfo, aryl or aralkyloxy. As examples of said lower alkoxy, halogen, aryl and aralkyloxy, there are mentioned those described above. As preferable examples of the group of the formula -CO-R7 substituted by lower alkoxy, amino, aryl or aralkyloxy, when substituted by lower alkoxy there is mentioned methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, n-butoxycarbonyl, sec-butoxycarbonyl and tert-butoxycarbonyl, when substituted by amino there is mentioned carbamoyl, when substituted by aryl there is mentioned benzoyl, when substituted by aralkyloxy there is mentioned benzyloxycarbonyl, respectively Preferred examples of R2 in the above formula are hydroxy, C1-6 lower alkoxy or -NH2. Examples of the above-mentioned C1-6 lower alkoxy are methoxy, ethoxy, propoxy, isopropoxy. n-butoxy, secbutoxy, tert-butoxy, n-pentyloxy, isopentyloxy and nhexyloxy. The nutnber of said substituents is preferably one to three. The hydrocarbon residue introduction reaction according to the invention is effected by using a halide carrying the desired hydrocarbon residue or an alkyl sulfate such as dimethyl sulfate. The halogen in said halide is, for example, bromine, iodine or chlorine. The reaction is generally carried out in a solvent such as tetrahycrosuran, dioxane, water, dimethyl formamide, chloroform, acetonitrile, ethyl acetate or pyridine, or in a mixture of such solvents. The alkylation reaction, for instance, is generally carried out in the presence of a base, such as an alkali metal hydroxide or sodium hydride, at a temperature generally in the range of about -100 to 800C (but not particularly limited thereto) for about 5 to 48 hours, preferably about 5 to 12 hours. In cases where the above-mentioned hydrocarbon residue R1 is an oxo-substituted alkyl, the above hydrocarbon residue introduction reaction may be carried out in the conventional manner of acylation. As the aclatin agent to be used in said acylation reaction, there is used an acylating agent containing an oxo-substituted alkyl group (acyl group) as represented by R1. Said acylating agent includes conventional acylating agents, such as halides, anhydrides, active esters and azides each derived from an acid containing said acyl group. The halogen of said halides is, for example, chlorine or bromine. Said active esters include, among others, p-nitrophenyl esters and N-hydroxysuccinimide esters. Said acylation reaction is preferably carried out in a solvent. Said solvent includes, among others, water, acetone, tetrahydrofuran, dioxane, acetonitrile, diethyl ether, chloroform, dichioromethane, ethyl acetate, dimethylformamide and pyridine. Such solvents may be used either alone or in the form of a mixture. The acylating agent is used generally in an amount of about 1 to 5 moles, preferably about 1 to 1.5 moles, per mole of the starging compound. Generally, the acylation is advantageously carried out in the presence of a base such as an alkali metal hydrogen carbonate, an alkali metal carbonate, an alkali metal hydroxide, a trialkylamine or pyridine. The reaction temperature is not critical. Generally, however, the reaction is carried out about -100 to +500C, preferably at about 5 to 300C, for about 1 to 48 hours, preferably about 1 to 12 hours. The pH in the reaction system is kept at about 7 to 11, preferably about 8 to 9. The esterification reaction in the practice of the invention can be carried out in the conventional manner. Thus, for instance, in accordance with one mode of carrying out said esterification reaction, the starting compound is suspended in an alcohol which contains an alkoxy group represented by R2 and, then, a gas such as hydrogen chloride gas is blown into the suspension. In this mode, the alcohol is used in large excess of the starting compound. The reaction temperature is generally about 0 to 800C, preferably about 25C to 600C, and the reaction time, namely aas introduction period, is about 1 to 12 hours, preferably about 1 to 4 hours. The esterification reaction may also be effected by suspending the starting compound in an alcohol (which contains an alkoxy group represented by R2) with an inorganic or organic acid, such as hydrogen chloride, sulfuric acid or toluene sulfonic acid, added thereto in advance, and treating the resulting mixture at about 0 to 1000C, preferably about 250 to 800C, or with heating under reflux, for about 5 to 48 hours, preferably 5 to 12 hours. In accordance with another mode of said esterification, an alcohol containing an alkoxy group represented by R2 is reacted with thionyl chloride, acetyl chloride, sulfinyl chloride, trifluoroacetic acid anhydride or the like at a low temperature of about -400 to -5 C, preferably about -200 to -50C, and then the starting compound is added to the above reaction mixture for treatment. The treatment (reaction) is carried out at about 0 to 1000C, preferably about 250 to 800C, or with heating under reflux, for about 1 to 48 hours, preferably about 4 to 12 hours. The amidation according to the invention is effected, for example, by bringing the above ester into contact with ammonia or an amine of the formula NH 2-R4 wherein R4 is as defined above. For said reaction, ammonia or the amine is used in an amount of about 1 to 20 moles, preferably about 2 to 10 moles, per mole of the ester. The reaction is carried out at about 0 to 1000C, preferably about 25 to 600C, for 12 to 72 hours, preferably 24 to 48 hours. The amidation may also be effected, for example, by activating the carboxyl group of the starting compound and then bringing the activation product into contact with ammonia or the above-mentioned amine. Said activation is effected by treating the starting compound, for example, with phosphorus pentachloride, thionyl chloride or the like for conversion of said compound to the acid chloride form, or with isobutyloxycarbonyl chloride, ethoxycrbonl chloride or the like for conversion of said compound to the mixed acid anhydride form, or by converting said compound to an active ester with p-nitrophenol, N-hydroxysuccinimide, N-hydroxy-5-norbornene-2, 3-dicarboximide or the like. The thus-activated starting compound is reacted with about 1 to 2 moles, per mole of the activated starting compound, of ammonia or the amine. The reaction is carried out at about 0 to 1000C, preferably about 25 to 60 C, for about 12 to 24 hours. In accordance with a further mode, the amidation may be carried out by contacting the starting compound with ammonia or the amine in the presence of a condensing agent such as a carbodiimide (e.g. dicyclohexylcarbodiimide), carbonylimidazole or N-ethyl-5-isoxazolium-3'- sulfonic acid salt. The condensing agent is used in an amount of 1 to 3 moles, preferably 1.2 to 1.5 moles, per mole of the starting compound. The reaction is carried out at about -10" to +500C, preferably about -5 to +250C, for about 5 to 48 hours, preferably about 10 to 24 hours. The thus-produced compound (I) is separated and/or collected from the reaction mixture by the conventional method, such as extraction with an organic solvent {e.g. chloroform, n-butanol) or column chromatography using an ion exchange resin or an adsorbent resin, among others. Those compounds (I) in which R1 is a substituted or unsubstituted hydrocarbon residue and R2 a group of the formula -OR3 wherein R3 is a hydrocarbon residue which may optionally be substituted or the formula -NH-R4 wherein is is a hydrogen atom or a hydrocarbon residue which may optionally be substituted can be produced by (i) bjecting the compound (II) or a salt thereof to hydrocarbon residue introduction reaction and then subjecting the resulting compound to esterification or amidation or (ii) subjecting the compound (II) or a salt thereof to esterifi- cation or amidation and then subjecting the resulting compound to hydrocarbon residue introduction reaction. The compound (III) in the optically active D form can be produced for example by the following procedure: EMI10.1 In the above formulas, the group R8 means a protective group other than acetyl. The protective group R8 is exemplified by t-butyloxycarbonyl, benzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl. Rg is a protective group which is eliminable under conditions different from those for eliminating the protective group R8. Examples of such protective group Rg are benzyloxycarbonyl, tert-butoxycarbonyl, p-nitrobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, trityl, tosyl, tert-amyloxycarbonyl, isobornyloxycarbonyl, diphenylphosphinyl, o-nitrophenylsulfenyl and phthaloyl. The compound (V) is a protected D-asparagine in which the a-amine group is protected by a protective group represented by R8. The compound (V) can be produced by reacting D-asparagine with a protective group introducing reagent containing R8 (R8-X wherein R is, for instance, halogen, active ester or amide). The reaction is carried out in water or a mixture of water and an organic solvent such as tetrahydrofuran, acetonitrile or dioxane, in the presence of a base such as an alkali metal hydroxide (e.g. NaOH, KOH) or triethylamine, at about OOC to 259C for about 1 to 24 hours. For the production of compound (VI) from compound (V), the compound (V) is reacted with bistrifluoroacetoxyphenyliodine in 2 mixed solvent composed of water and dimethylformamide. The reaction is carried out in the presence of a base, such as triethylamine or an alkali metal hydroxide, at about 0 to 500C, preferably about 100 to SOC, for about 10 to 48 hours. For the production of compound (VII) from compound (VI), the compount (VI) is acylated at the amino group thereof with an agent for introducing a protective group represented by Rg . Thus, said protective group (Rg-) introducing agent and the compound (VI) are reacted in water, tetrahydrofuran, acetonitrile or dimethylformamide or a mixed solvent composed of these, at about 0 to 500C for about 5 to 24 hours. The reaction is generally carried out in the presence of a base such as triethylamine or sodium hydrogen carbonate. For the production of compound (VII I) from compound (VII), the carboxylic acid is first converted to a mixed acid anhydride, which is then diazonized and then subjected to Wolff rearrangement. The rixro acla anr.ycrade is producer by rca-tang tEle compound (VII) with an acid chloride such as ethyl chlorocarbonate, methyl chlorocarbonate, propyl chlorocarbonate, butyl chlorocarbonate or isobutyl chlorocarbonate, in an organic solvent such as ethyl acetate, methyl acetate, dioxane, tetrahydrofuran or acetonitrile. The reaction may be ca-rried out in the presence of an organic base such as N-methylmorpholine, N-ethylmorpholine, triethylamine, trimethylamine or pyridine. The reaction temperature is about -200C to 30 C, preferably about -10 C to OOC. The diazonation is carried out by reacting the mixed acid anhydride with diazomethane in an organic solvent such as ethyl acetate, methyl acetate, tetrahydrofuran, dioxane, acetonitriel or diethyl ether, at a temperature of about -200C to 300C, preferably about -10 C to 250C, for about an hour to 24 hours. Diazomethane may either be blown into the solution or be added to the solution as a saturated solution of diazomethane in an organic solvent such as diethyl ether or ethyl acetate. The Wolff rearrangem.ent is carried out by reacting the diazonized compound with a silver salt of benzoic acid or acetic acid, for instance, dissolved in an organic base such as trimethylamine, triethylamine, N-methylmorpholine, N-ethylmorpholine or pyridine, at about -5 C to 500C, preferably about 0 C to 270C, in bye dark, for about 30 minutes to 10 hours. The compound (IX) is produced by saponifying the compound (VIII). Said saponification is carried out by contacting the compound (VIII) with a hydroxide of sodium, potassium or barium, for instance, in water, an organic solvent such as methanol, ethanol, dioxane, tetrahydrofuran or acetonitrile, or a mixed solvent composed of water and an organic solvent such as mentioned above, at about -100C to 500C, preferably about 00C to 270C, for about an hour to 5 hours. The compound (X) is produced from the compound (IX) by selective elimination of R8 and introduction of acetyl group. Said elimination can be performed by a method conventionally used in the peptide syntheses such as hydrolysis, catalytic reduction, or acid treatment. The hydrolysis can be effected by any method that is conducive to a cleavage of an amide bond. For example, methods employing an acid, a base or an ion exchange resin may be mentioned. Examples of said acid include inorganic acids such as sulfuric acid and hydrochloric acid and examples of said base include potassium hydroxide, sodium hydroxide and barium hydroxide. Examples of said ion exchange resin include Dowex-50 (Dow Chemical, U.S.A.), Amberlite IR-120 (Rohm and Haas Co., U.S.A.) and Diaion-SKlA nad S1B (Mitsubishi Chemical Industries Ltd., Japan). When the acid is employed, the reaction is preferably conducted in aqueous solution and when an aqueous solvent is employed, it is preferably a mixture of water with methanol, ethanol, butanol or the like. The reaction is conducted generally at about 60 to 2000C and preferably at about 90 to 1200C, generally for about 30 minutes to 30 hours and preferably for about 3 to 16 hours. When the base is employed, the reaction is desirably conducted in aqueous solution. This reaction is also desirably conducted in an aqueous solvent such as a mixture of water with methanol, ethanol, butanol or the like. This reaction is carried out generally at about 60 to 2000C and preferably at about 90 to 1200C, generally for about 30 minutes to 30 hours and preferably for about 3 to 16 hours. When the ion exchange resin is employed, the resin is suspended in an aqueous solution of the starting material compound and the suspension is heated. This reaction is conducted generally at 60 to 2000C and preferably at about 90 to 1200C, generally for about 30 minutes to 30 hours and preferably for about 3 to 16 hours. Said catalytic reduction is carried out in an alcohol such as methanol or ethanol or a mixed solvent composed of water and an alcohol, in the presence of a catalyst such as palladium black or palladium-on-carbon introducing hydrogen gas into the reaction system, if necessary under pressure. The reaction is carried out at OOC to 500C, preferably at 20 to 300C, and the reaction time is within about 0.5 to 5 hours, preferably within about 1 to 3 hours. The acid to be used in said acid treatment is, for example, hydrogen bromide-acetic acid, hydrochloric acidacetic acid, hydrochloric acid-dioane, trifluoroacetic acid, methanesulfonic acid or trifluoromethanesuifonic acid. The temperature of the acid treatment should advisably be selected depending upon the acid used witin the range of about -10 C and 50 C, preferably about OOC to 25 C, and the reaction time within the range of about 30 minutes to 24 hours. The acetylation is effected by reacting the starting compound with acetic anhydride, or a mixed acid anhydride prepared by reacting acetyl chloride or acetic acid with an alkoxycarbonyl chloride such as methyl chiorocarbonate, ethyl chlorocarbonate, butyl chlorocarbonate, propyl chlorocarbonate or isobutyl chlorocarbonate, in water or a mixed solvent composed of water and an organic solvent such as acetone, dioxane, acetonitrile, dimethylformamide or tetrahydrofuran. The reaction may be conducted in the presence of a deacidifying agent such as an organic base (e.g. pyridine, triethylamine, trimethylamine) or a hydroxide, oxide or bicarbonate of an alkali or alkaline earth metal (e.g. sodium, potassium, calcium). The reaction temperature is about -100C to 500C, preferably about OOC to 250C. The trimethylation of compounds (III') from the compound (X) in accordance with the present invention is carried out, for example, by reacting compound (X) with dimethyl sulfate, methyl bromide, methyl chloride or methyl iodide in water or a mixture of water and an organic solvent such as acetonitrile, dioxane, tetrahydrofuran or dimethylformamide. The reaction may be performed in the presence of, for instance, a hydroxide or oxjde of an alkali or alkaline earth metal such as sodium, potassium or calcium, as necessary. The reaction temperature is about -100C to 500C, preferably about OOC to 200C. The desired products yielded by either of the above reactions can be isolated and purified by conventional separation and/or purification techniques, such as chromatography, recrystallization, etc. The compound (III") is produced by hydrolysis of the compound (III'). The hydrolysis can be effected by any method that is conductive to a cleavage of an amide bond. For example, methods employing an acid, a base or an ion exchange resin may be mentioned. Examples of said acid include inorganic acids such as sulfuric acid and hydrochloric acid and examples of said base include potassium hydroxide, sodium hydroxide and barium hydroxide. Examples of said ion exchange resin include Dowex-50 (Dow Chemical, U.S.A.), Amberlite IR-120 (Rohm and Haas Co., U.S.A.) and Diaison-SKlA and SKlB (Mitsubishi Chemical Industries Ltd., Japan). When the acid is employed, the reaction is preferably conducted in aqueous solution and when an aqueous solvent is employed, it is preferably a mixture of water with methanol, ethanol, butanol or the like. The reaction is conducted generally at about 60 to 2000C and preferably at about 90 to 1200C, generally for about 30 minutes to 30 hours and preferably for about 3 to 16 hours. When the base is employed, the reaction is desirably conducted in aqueous solution. This reaction is also desirably conducted in -an aqueous solvent such as a mixture of water with methanol, ethanol, butanol or the like. This reaction is carried out generally at about 60 to 2000C and preferably at about 90 to 1200C, generally for about 30 minutes to 30 hours and preferably for about 3 to 16 hours. When the ion exchange resin is employed, the resin is suspended in an aqueous solution of the starting material compound and the suspension is heated. This reaction is conducted generally at 60 to 2000C and preferably at about 90 to 1200C, generally for about 30 minutes to 30 hours and preferably for about 3 to 16 hours. Isolation of deacetyl-FA-5859 or a salt thereof from the reaction product mixture can be accomplished by the per se conventional procedures such as ion exchange, adsorption, concentration, crystallization, etc. While the desired compound can be isolated optionally as the free compound or as a salt thereof, it is more expedient to isolate it in the form of a salt. A typical procedure for isolating the desired substance from the reaction mixture comprises adsorbing the substance on a strongly acidic ion exchange resin or the like, desorbing the same with hydrochloric acid or the like and collecting ninhydrin-positive fractions. When the hydrolysis is effected with hydrochloric acid for instance, a more expedient procedure may be adopted. Thus, the reaction mixture is concentrated under reduced pressure to remove the excess hydrochloric acid and a solvent such as methanol, ethanol or diethyl ether is added to the residue to give hydrochloride of the substance as crystals. By the process described above is obtained the compound (III) as the free compound or as a salt. The optically active L form of compound (II), which is the starting compound to be used in practicing the invention is deacetyl-FA-5859. Deacetyl-FA-5859 can be produced, for example, by cultivating a microorganism be longing to the genus Emericella and capable of producing FA-5859 in a culture medium so as to cause formation and accumulation of FA-5859 in the culture medium, collecting the same and subjecting the same to hydrolysis. The chemical synthesis of the D form of the starting compound (II) for the method according to the invention is as mentioned hereinabove as a method of producing compound (III) from compound (V). As for the L form, it can be produced in the same manner by using the L form of compound (V). As the microorganism to be used in producing FA-5859 and compound (IV), there may be used, for instance, any microorganisms which belong to the genus Emericella and are capable of producing FA-5859 and/or compound (IV). As an example of such microorganism, there is mentioned Emericella quadrilineata, and more particularly, Emericella quadrilineata IFO 5859. The above-mentioned IFO 5859 strain was deposited on September 14, 1954 at the Institute For Fermentation, Osaka (IFO), Japan, and is listed in the Institute For Fermentation, Osaka (IFO) List of Cultures, 1978, Sixth Edition published by the IFO. The microbiological characteristics of Emericella guadrilineata are as described in Transactions of The Mycological Society of Japan, Volume 20, No. 4, 481 (1979). As it is true of microorganisms in general, microorganisms of the genus Emericella are liable to undergo mutation whether spontaneously or under the influence of a mutagen. Thus, any and all mutants that are obtainable by irradiation with X-rays, gamma rays, ultraviolet light, etc., monospore separation, treatment with certain reagents or cultivation in media containing such reagents, or other mutagenic treatments, as well as those spontaneous mutants that may be available, can be successfully employed in the production of FA-5859 and/or the compound (IV) when they are still capable of elaborating them. The medium used for the production of FA-5859 may be liquid or solid, insofar as it contains nutrients available to the strain employed, although a liquid medium is suited for mass production. In the medium are incorporated suitable proportions of assimilable carbon sources, digestable nitrogen sources, inorganic substances and trace nutrients. The carbon sources may for example be glucose, lactose, sucrose, maltose, dextrin, starch, glycerin, mannitol, sorbitol and oils and fats (e.g. soybean oil, olive oil, bran oil, sesame oil, lard oil, chicken oil, etc.), fatty acids (e.g. lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, etc.). The nitrogen sources may for example be meat extract, yeast extract, dried yeast, soybean flour, corn steep liquor, peptone, cottonseed flour, spent molasses, urea, ammonium salts (e.g. ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium acetate, etc.) and so on. In addition, salts including those of sodium, potassium, calcium, magnesium, etc., metal salts such as those of iron, manganese, zinc, cobalt, nickel, etc., salts of phosphoric acid, boric acid, etc., and salts of organic acids such as acetic acid, propionic acid, etc. are also used as necessary. It is, of course, possible to add amino acids (e.g. glutamic acid, aspartic acid, alanine, lysine, valine, methionine, proline, etc.), peptides (e.g. dipeptides, tripeptides, etc.), vitamins (e.g. B1, B2, nicotinic acid B12 C, etc.), nucleic acids (e.g. purine and pyrimidine and their derivatives) and others. Of course, for the purpose of controlling the pH of the medium, an organic or inorganic acid, an alkali, a buffer solution or the like may be added. For defoaming purposes, an oil, a surfactant or the like may also be added to the medium. The cultural methods that may be employed include stationary culture, shake culture or aerobic submerged or stirred culture, for instance. For mass production, submerged aerobic culture is of course preferable. While the conditions of cultivation, of course, depends on the species or strain of microorganism, the cultural method used and so on, fermentation is generally conducted at a temperature of about 15 to 370C with the initial pH being set at pH about 3 to 8. Particularly desirable conditions are about 23 to 320C in the intermediate stage of cultivation and pH about 4 to 6 at the start of cultivation. While the cultivation time is also dependent on the abovementioned conditions, it is desirable to continue cultivation until the titer of the physiologically active substance has reached a maximum. In the case of shake culture or submerged aerobic culture in a liquid medium, the duration of time to such maximum titer is generally about 1 to 8 days. The FA-5859 and the compound (IV) thus elaborated occurs mostly in the liquid phase of the fermentation broth. Therefore, it is a recommend procedure to separate the broth into a supernatant and a cellular mass by filtration or centrifugation and purify the supernatant to recover the desired substance. However, it is also possible to subject the fermentation broth as such directly to a known purification procedure. To harvest FA-5859 and/or the compound (IV) from the broth, the procedures generally known for the isolation of microbial metabolites can be employed. ror example, microbial cells are removed by centrifugation and the active product is separated and purified from the supernatant fluid. Thus, such procedures as the one utilizing solubilities or a difference in solubility in various solvents, precipitation from a solution, the method utilizing differential rate of precipitation, a difference in adsorptive affinity for a given adsorbent, ion exchange chromatography on ion exchangers, concentration under reduced pressure, crystallization, recrystallization, drying, etc. can be utilized either singly or in a suitable combination or in repetition. The compound (I), (III) and (IV) each can be converted to a pharmaceutically acceptable salt by the established procedure. As examples of the acid used for this conversion to a salt, there may be mentioned hydrochloric acid, sulfuric acid, nitric acid, oxalic acid, acetic acid, succinic acid, citric acid, fumaric acid, etc. When the compound (I), (III) and (IV) each is obtained as a salt form, it can be converted to a free form by the established procedure, such as a method employing an ion-exchange resin to absorb the acid or base which forms the salt. Then, the fatty acid degradation inhibiting activity of the compound (I), (III), (IV) was determined in accordance with the method described in P.75 "Metabolism of Lipids" which is Vol. 9 of "Seikagaku Jikken Koza" (Text of Biochemical Experiments)compiled by Tokyo Kagaku Dojin, published 1975, using rat liver homogenates. Thus, an SD strain rat (6 weeks of age, male) was fasted for one day and, then, bled to death. The liver was immediately excised and homogenized with 10 times (w/v) of a 0.25 M sucrose solution containing a 5 mM Tris-HCl buffer (pH 7.4), 0.1 mM EDTA and 1 mg/ml bovine serum albumin using a Teflon rod homogenizer. The homogenate was centrifuged at 820 xg for 10 minutes and the supernatant was further centrifuged at 8,000 xg for 10 minutes. The resultant pellets were suspended in the same sucrose solution as above to a concentration of 0.2 g wet liver weigh=/0.5 ml solution and 0.5 ml of the suspension was used as the enzyme solution in the reaction. Then, 2.5 ml of a reactant mixture comprising 30 umole of potassium phosphate buffer (pH 7.4), 300 vmole of KC1, 3 pmole of ATP, 3 pmole of MgC12, 120 vmole of sucrose, 0.6 Umole of 1-14C palmitic acid (0.2 pCi and bovine serum albumin withamolar ratio of 1: :5, pH 7.4), 3 mole of L-carnitine, 0.6 umole of Co-enzyme A, 7.5 vmole of NAD, 0.03 vmole of oxalacetic acid, 0.1 ml of water or an aqueous solution containing an inhibitor and 0.5 ml of the enzyme solution was incubated aerobically at 370C for 20 min. in a sealed tube hanging a filter paper immersed with Hyamine Hydroxide 10-X (Packard, Holland) and the reaction was stopped by adding 0.4 ml of 70% perchloric acid. 4C02 formed was trapped and the radioactivity was counted. The inhibitory activity measured is shown in Table 1. The inhibitory activity is shown as percentage of inhibitory activity to degradation activity of fatty acid facilitated by the addition of carnitine to that of not added of the compound. Table 1. Inhibitory effects derivatives ss-amino-y- trimethylammonio-butyrate on activity of fatty acid degradation which depends own caanitine added. Inhibition (%) Compound (I), (III), (IV) Concentration of drug R1 (R2= -OH) Salt 2 mM 0.2 mM 20 pM 2 pM -COH 84 53 -COCH3(D-) HC1 28 2 -COCH2CH3 HC1 44 -CO(CH2)2CH3 50 -CONH2 HC1 72 34 -COOCH2C6H5 98 85 -CO (CH2) 14CH3 100 100 -H (D-) 2HC1 36 11 -CH3 100 89 Inhibition % Compound (I) Concentration of drug R2 (R1= H) Salt 0.2 mM 20 UM 2 UM -OCH3 2HC1 73 26 -OCH2CH3 2HC1 85 37 -O(cH2)2CH3 2HC1 69 41 -NH2 2HC1 67 27 10 Antiketogenic activities of derivatives of ss-amino-y- trimethylammonio-butyrate were measured as follows: After they were fasted for 20 hours, male Sprague-Dawley rats ( 8 weeks old, n=5) were orally given the compounds. which were suspended in 5% gum arabic, at a dose of 50mg/kg. Blood samples were taken 4 hours later from a vein of tail and ketone bodies were measured as ss-hydroxybutyrate according to the modified method of Williamson L D.H.Williamson et al. ; Methods of Enzymatic Analysis, P454 (1968), Academic Press, New Yorkl. The amount of ketone bodies was shown as a relative amount of ss-hydroxybutyrate (percentage) to the amount of p-hydroxybutyrate in blood of control rats. Results obtained are shown in Table 2. As seen from the data, good relationship between inhibitoy activity of fatty acid degradation and antiketogenic activiy of the derivatives of B-amino-y-trimetylaminobutyrate was o$served. Table 2. Antiketogenic activities of derivatives of 8-amino-y-trimethlammoniobutyrate (n=5) Compound (I), (III), (IV) relative amount of R1 (R2= -OH) Salt ss-hydroxybutylate (%) -COH 75.7 -CONH2 HC1 63.2 -COOCH2C6H5 19.7 -co(cs,)14CH3 6.7 -CO (CH2) 12CH3 7.9 -CH3 19.9 Compound (I) relative amount of R2 (R1=H) Salt ss-hydroxybutylate (%) -OCH2CH3 2HC1 5.9 In diabetes, blood level of ketone bodies usually increases and so-called ketonemia or ketoacidosis often occurs. This abnormal increase of ketone bodies gives severe damages to patients, such as coma. Consequently, the present compounds, which have antiketogenic activity, are of value as new drugs for the treatment of ketonemia or ketoacidosis. In particular, it is presumed that the compound (I) wherein R1 is alkyl hardly causes side effect of inducing fatty liver, and that inactivation of the compound by enzymes is decreased. Therefore, the compound (I) wherein R1 is alkyl is advantageously used as a drug. As shown in Tables 1 and 2, the present compound shows a remarkable fatty acid decomposition inhibitory activity and antiketogenic activity. Toxicity of the present compound is low, because there is not found dead mouse by 400 mg/kg oral administration. Therefore, the compounds (I), (III), (IV) or salts thereof is useful as a fatty acid decomposition inhibitor, for instance. When the compound (I), (III), (IV) or a salt thereof is used as such a fatty acid decomposition inhibitor for the treatment of diabetes in mammalian animals (e.g. mouse, rat, man), it is administered at a daily dose of about 0.2 to 200 mg/kg. The compound (I), (III), (IV) or a salt thereof can be administered orally as such or in combination with a pharmaceutically acceptable carrier, excipient or diluent in such dosage forms as tablets, granules, capsules, liquids, etc., or non-orally in the form of an injectable preparation. In the production of oral preparations, there may be employed suitable amounts of binders (e.g. hydroxypropyl-cellulose, hydroxypropylmethyl-cel lulose, macrogol, etc.), disintegrators (e.g. starch, carboxymethyl cellulose calcium, etc.), excipients (e.g. lactose, starch, etc.), lubricants (e.g. magnesium stearate, talc, etc.) and so on. In the production of parenteral or non-oral preparations, e.g. injections, there may be employed iso tonicating agents (e.g. glucose, D-sorbitol, D-mannitol, sodium chloride, etc.), preservatives (e.g. benzyl alcohol, chlcrcDut2nol, methyl p-hydroxybenzoate, propyl pphydroxybenzoate, etc.), buffers (e.g. phosphate buffer, sodium acetate buffer, etc.) and so on. Brief Description of the Drawings Fig. 1 is an infrared absorption spectrum of phislo- logically acitve substance FA-5859 as obtained in Reference Example 2, and Fig. 2 is an infrared absorption spectrum of the hydrochloride of physiologically active substance FA-5859 as obtained in Reference Example 3. The following Reference Examples and Examples are further illustrative of this invention. In the Reference Exarples, percent figures with respect to medium compositions are on a weight/voLume basis. And in the Examples, the following abbreviations are used. MeOH: methanol EtOH= ethanol AcOEt: acetic acid ethyl ester A2pr : residue of diaminopropionic acid A2bu : residue of ss,&gamma;-diaminobutyric acid OMe : methyl ester Z : benzyloxycarbonyl BOC : t-butoxycarbonyl Ac : acetyl Reference Example 1 A loopful of Emericella quadrilineata IFO 5859 grown sufficiently to sporulate on a potato-sucrose-agar slant was used to inoculate a 2-liter Sakaguchi flask containing 500 ml of a sterilized medium composed of 2.0t glucose, 3.08 maltose, 1.5% raw soybean flour, 1.0% corn steep liquor, 0.5% Polypepton (Daigo Nutritive Chanicals, Japan), 0.3% yeast extract and 0.3t sodium chloride (pH 6.0), and was incubated on a reciprocating shaker at 280C for 2 days. Then, 1.5 R of this seed culture was added to 100 Q of a fermentation medium composed of 3.0% oleic acid, 0.5t raw soybean flour, 0.5% malt extract, 0.5% Polypepton, 0.2% yeast extract, 0.18 KH2PO4, 0.05% FeSO4-7H2O, 0.05% MnSO4.nH2O and 0.05S MgSO4.7H2O (pH 4.5) in a 200-Q fermentation tank. This fermentation was conducted at 280C, 100 Q/min. aeration, 200 r.p.m. agitation and an internal pressure of 1.0 kg/ cm2 for 114 hours. A couple of batches of fermentation thus obtained were pooled and the cells were removed by filtration to give a filtrate containing FA-5859. Reference Example 2 A 125 Q portion of the filtrate obtained in Example 1 was passed through a column of Amberlite IR-120 (H form) (20 Q) and after the column was rinsed with 40 Q of water, elution was carried out with 1N-aqueous ammonia. The eluate was concentrated under reduced pressure to a volume of 30 Q to remove the ammonia and the concentrate was passed through a column of chromatographic grade activated carbon (30 Q). The column was rinsed with 60 g of water and elution was carried out with 90 g of 50% aqueous methanol. The eluate was collected in 10 ut fractions and the active fractions No. 5 to No. 6 were combined and concentrated under reduced pressure to give 25.5 g of a crude syrup. This crude product was dissolved in 100 ml of acetate buffer (pH 4.0) (0.05 M) and the solution was passed through a column of Dowex 50 x 2 (500 ml) buffered with acetate buffer (0.1 H) (pH 4.0). Then, elution was performed with the same buffer as above in the order of 1 L at pH 4.0, 1.5 L at pH 4.3, 1.5 t at pH 4.6 and 1.5 Q at pH-5.0. The eluate was collected in 100 ml fractions and the fractions No. 32 to No. 63 were pooled and passed through a column of Amberlite IR-120 (H form) (300 ml). After the column was rinsed with 600 ml of water, solution was carried out with 1.5 t of 0.5N-aqueous ammonia. The eluate was concentrated under reduced pressure to a volume of 500 ml and the concentrate was passed through a column of Dowex 1x2 (OB form) (200 ml), followed by washing with 200 ml of water. The effluent and washings were combined and concentrated under reduced pressure and lyophilized. Allowing the syrup to stand at room temperature yielded 10.7 g of colorless hygroscopic crystals of FA-5859 (free form). The infrared absorption spectrum of this product is reproduced in Fig. 1. Reference Example 3 In 10 ml of water was dissolved 210 mg of FA-5859 free form produced in Reference Example 2, and under icecooling, 1 ml of lN-HCl was added. The mixture was concentrated under reduced pressure and after addition of 10 ml of ethanol, allowed to stand at room temperature. The resultant crystals were recrystallized from waterethanol to give 225 mg of hydrochloride of FA-5859 as colorless needles. m.p. 2150C (decompn.). The infrared absorption spectrum of this compound is shown in Fig. 2. The physico-chemical properties of FA-5859 (free form) as obtained in Refernece Example 2 which appears hereinafter are as follows. (a) Elemental analysis (%) (after drying over phosphorus pentoxide under reduced pressure at 600C for 10 hours) C : 52.48% H : 9.04% N : 13.25% (b) Molecular weight: 2.4-3.3 X102 (H2O) (by VPO method) (c)! Empirical formula: C9H18N2O3 (d) Specific rotation: [a]23 - 17.4 (c=l, H20) D (e) Ultraviolet absorption spectrum: No characteristic absorptions at wavelengths over 210 nm. (f) Infrared absorption spectrum: Principal absorptions (wave-numbers) are as follows: 3420(s), 3260(sh) , 3080(m), 1660(s), 1590(s), 1485(s), 1400(s), 1325(m), 1295(m), 1145(w), 1105(w), 1060(w), 970(m) , 945(m), (cm w: weak, m: medium, s: strong, sh: shoulder Refer to Fig. 1 (potassium bromide disk) (g) Solubilities: Insoluble: Petroleum ether, hexane, diethyl ether, benzene, ethyl acetate, chloroform Hardly soluble: Pyridine, acetone, dimethyl sulfoeide dimethylformamide Soluble: Ethanol, methanol Readily soluble: Water (h) Color reactions Positive: Iodine reaction Negative: Greig-Leaback, ninhydrin, Sakaguchi, Molisch and Ehrlich reactions (i) Basic, acidic or neutral: Amphoteric (j) Color of the substance: Colorless (k) Appearance of crystals: Colorless hygroscopic crystals (1) Nuclear magnetic resonance spectrum (CD30D, 100 MHz): 1.98(3H;s), 2.42(2H,d), 3.19(9H,s), 3.56(2H,d), 4.7 (lH,m) s: singlet, d: doublet, m: multiplet (m) Stability: Stable in aqueous solution at pH 3 to 9 under heating at 1000C for 10 minutes. The physico-chemical properties of FA-5859.hydrochloride as obtained in Reference Example 3 which appears hereinafter are as follows: (a) Elemental analysis (%): (after drying under reduced pressure over phosphorus pentoxide at 600C for 10 hours) C : 45.29% H : 8.18% N : 11.24% C1: 14.36% (b) Empirical formula C9H18N2O3-HCl (c) Melting point : 215"C (decompn.) (d) Specific rotation: 2320.50 (c=l, H20) D (e) Ultraviolet absorption spectrum: No characteristic absorptions at wavelengths over 210 nm. (f) Infrared absorption spectrum: Dominant absorptions (wave-numbers) are as follows. 3400(m), 3250(s), 3190(sh), 3045(s), 2600-2400(w), 1730(s) . 1660(s) , 1530(m) , 1480(s), 1420(m), 1405(s), 1375(m), 1290(m), 1205(m), 1160(s), 1140(sh), 1135(s), 1040(w), 960(w), 935(m), 915(m), 865(w), 800(m), 665(m), 625(w), 600(s), 560(w) (cm 1) (w: weak, m: medium, s: strong) Refer to Fig. 2 (potassium bromide disc) (g) Solubilities Insoluble: petroleum ether, hexane, diethyl ether, benzene, ethyl acetate, chloroform Hardly soluble: pyridine, acetone, dimethyl sulfoxide, dimethyl formamide Soluble: ethanol, methanol Readily soluble: water (h) Color reactions: Positive: iodine reaction Negative: Greig-Leaback, ninhydrin, Sakaguchi, Molisch and Ehrlich reactions (i) Color of the substance: colorless (j) Appearance of crystals: colorless needles (k) Stability: Aqueous solutions at pH 3 to 9 are stable at 1000C for 10 minutes. The molecular formula of FA-5859 and the NMR signal at 6 3.19 ppm (9H, s) suggest the presence of a trimethylammonium group in the molecule. Moreover, the NMR spectrum indicates the presence of methyl protons of the acetyl group (CH3CO-) at a 1.98 ppm (3H, s) and a couple of methylene protons (-CH2-x2) at 6 2.42 ppm (2H, d) and 3.56 ppm (2H, d) and a methine proton (-CH-) at d 4.7 ppm. Decoupling studies reveal that the above couple of methylene protons are respectively coupled with the methine proton at 6 4.7 ppm, suggesting the existence of a partial structure of -CH2-CH-CH2-. Moroever, the molecular formula of this compound suggests the presence of a carboxyl group. This is also apparent from the fact that a C=O vibration is seen at 1590 cm 1 in the case of the free compound and at 1730 cm 1 in the case of the hydrochloride. Therefore, the following planar structural formula may be advanced for FA-5859. EMI29.1 FA-5859 is in the optically active L form, because of its specific rotation. The salts is also L-form. Reference Example 4 In 40 ml of constant boiling point hydrochloric acid was dissolved 1.60 g of free FA-5859 obtained in Reference Example 2 and the solution was allowed to stand at 950C for 16 hours. The reaction mixture was concentrated under reduced pressure and the residue was diluted with a small amount of water and concentrated under reduced pressure for a second time. To the residue was added a mixture of methanol and diethyl ether and the resultant crystals were collected by filtration. Recrystallization from methanol gave 1.20 g of deacetyl-FA-5859.2HCl. m.p. 219-220"C; [a122+6.3 (c=1.0, lN-AcOH). Elemental analysis: C7H180 2N2C12 Calcd.: C, 36.05; H, 7.77; N, 12.01; C1, 30.40(%) Found : C, 36.09; H, 7.72; N, 11.81; C1, 29.80(%) Absoprtion spectrum: No characteristic absorption over the ultraviolet and visible region from 210 nm to 700 nm. The product is assumed to be in the optically active L-form, because of its specific rotation. Example 1 Production of [(R) -3-carboxy-2-formylaminopropyl] - trimethylammonium picrate: A solution of 930 mg of deacetyl-FA-5859 dihydrochloride obtained in Reference Example 4 (the same was used in Examples 1 to 12 and 14)in 10 ml of formic acid was cooled to OOC, and 336 mg of NaHC03 and 2.8 ml of acetic anhydride were added. The mixture was stirred for an hour and then at room temperature for 4 further hours. The reaction mixture was cooled, and 50 ml of water was added. After solvent removal by distillation under reduced pressure, the residue was dissolved in 100 ml of water and the solution was passed through a Dowex 50W x 2 colum (H+ form; 80 ml). After washing the column with 300 ml of water, elution was performed with 0.5 N aqueous ammonia. The eluate was evaporated under reduced pressure to remove the solvent and the residue, which weighed 880 mg, was dissolved in 10 ml of methanol. To the solution was added a solution of 1.10 g of picric acid in 30 ml of ethanol, and the resulting precipitate was collected by filtration. Recrystallization from methanol gave 1.25 g of the title compound. 24 Melting point: 174-1760C, [&alpha;]D -3.3 (c=1.0, dimethylformamide). Elemental analysis Calcd. for C4H19N5O10: C, 40.29; H, 4.59; N, 16.78% Found: C, 40.18; H, 4.64; N, 16.75% Example 2 Production of [(R)-3-carboxy-2-propionylaminopropyl]- trimethylammonium chloride: A solution of 700 mg of deacetyl-FA-5859 dihydrochloride in 20 ml of water was cooled to OOC, and 1.10 g of NaHCO3, 20 ml of acetonitrile and 0.58 ml of propionic anhydride were added. The mixture was stirred at 0 C for an hour and then further at room temperature for 5 hours. The acetonitrile was distilled off and 100 ml of water was added. The mixture was passed through a Dowex 50 W x 2 column (H+ form; 80 ml). After washing the column with 240 ml of water, elution was performed with 0.5 N ammonia water. The eluate was evaporated under reduced pressure to remove the solvent and the residue (0.63 g) was dissolved 2.8 ml of 1 N HCl. After removel of the water by distillation, the residue was treated with methanol-ethyl ether to give 0.59 g of the title compound as colorless crystals. Melting point: 161-1630C, [&alpha;]24 - 16.7 (c=l.Q, water) D Elemental analysis Calcd. for C10H21N2O3Cl: C, 47.52; H, 8.37; N, 11.08; C1, 14.03E Found: C, 47.18; H, 8.40; N, 10.90; C1, 13.888 Example 3 Production of [R)-3-carboxy-2-n-butyrylaminopropyll- trimethylammonium picrate: A solution of 700 mg of deacetyl-FA-5859 dihydrochloride in 20 ml of water was cooled, and 1.10 g of NaHCO3, 20 ml of acetonitrile and 0.7 ml of n-butyric anhydride were added. The mixture was stirred at OOC for an hour and then at room temperature for an additional 6 hours. The acetonitrile was distilled off and the residue was dissolved in 100 ml of water. The solution was passed through a Dowex 50 Wx2 column (H+ form; 80 ml). After washing the column with 300 ml of water, elution was performed with 0.5 N aqueous ammonia. The eluate was evaporated under reduced pressure to remove the solvent and the resulting oily product (0.65 g) was dissolved in 10 ml of methanol, followed by addition of a solution of 1.0 g of picric acid in 40 ml of ethanol. The resulting crystalline precipitate was collected by filtration and recrystallized from ethanol to give 0.91 g of the title compound. Melting point: 176-177"C, Ia3D -0.9" (c=1.0, dimethylformamide). Elemental analysis Calcd. for C17H25N5 10 C, 44.44; H, 5.49; N, 15.29% Found: C, 44.22; H, 5.22; N, 15.30% Example 4 Production of (R) - 3-palmitoylamino- 4- trimethylammonio- butyrate: A solution of 446 mg of deacetyl-FA-5859 dihydrochloride in 20 ml of water was cooled, and 8 ml of 1 N NaOH and 20 ml of acetonitrile were added, followed by dropwise addition of 550 mg of palmitoyl chloride with stirring. The mixture was stirred at OOC for one hour and then at room temperature for an addition 10 hours and the insoluble matter was filtered off, followed by removal of the acetonitrile by distillation under reduced pressure. The residue was acidified with 1 N HC1 and extracted with n-butanol (50 mlx2). After removal of the n-butanol by distillation under reduced pressure, the residue was dissolved in a small amount of water and the Insoluble matter was filtered off. The filtrate was lyophilized to give 105 mg of a colorless powder. This powder (90 mg) was dissolved in 10 ml of 5% (v/v) n-butanol-water and the solution was passed through a column of Amberlite IRA-402 (Rohm and Haas Company, U.S.A.) (acetic acid form, 5 ml). The effluent and aqueous washings were combined, concentrated and lyophilized to give 81 mg of the dihydrate of the title compound. 1aj24 11.20 (c=0.5, MeOH) Elemental analysis Calcd. for C23H46N203.2H2O: C, 63.55; H, 11.59; N, 6.449 Found: C, 63.82; H, 11.10; N, 6.13% Example 5 Production of [(R)-3-carboxy-2-methoxycarbonylaminopropyl]trimethylammonium chloride: To a cooled solution of 816 mg of deacetyl-FA-5859 dihydrochloride in 20 ml of water, there were added 1.29 g of NaHCO3 and 395 mg of methoxycarbonyl chloride. The mixture was stirred at OOC for 30 minutes and then at room temperature overnight. The reaction mixture was neutralized with 1 N HC1 and then washed with 50 ml of AcOEt. The aqueous layer was chromatographed on a Dowex 50 W x 2 column (H form, 100 ml). After washing the column with 400 ml of water, elution was effected with 0.5 N aqueous ammonia. The eluate was concentrated under reduced presusre, and the residue was dissolved in 30 ml of water. The solution was passed through an Amberlite IRC-50 column (H+ form, 10 ml). The column was washed with 200 ml of water. The effluent and washings were combined and evaporated under reduced pressure. The oil (0.75 g) thus obtained was dissolved in 3 ml of 1 N HCl, followed by evaporation again under reduced pressure. Crystallization of the residue by addition of ethyl ether gave 0.65 of the title compound. Melting point: 201-205"C (decomposition) 25 [alD5 - 22.30 (c=0.75, water} Elemental analysis Calcd. for C9H19N2O4Cl: C, 42.43; H, 7.52; N, 10.99; C1, 13.92% Found: C, 42.10; H, 7.23; N, 10.64; Cl, 13.84% Example 6 Production of (R)-3-benzyloxycarbonylamino-4-trimethyl ammoniobutyrate: To a cooled solution of 1.63 g of deacetyl-FA-5859 dihydrochloride in 28 ml of 1 N NaOH, there were added 5 ml of acetonitrile and 1.70 g of benzyloxycarbonyl chloride. The mixture was stirred vigorously at 0 C for 2 hours, followed by further stirring at room temperature for 12 hours. The reaction mixture was washed with 50 ml of ethyl ether, and the aqueous layer was chromatographed on a Dowex 50 Wx2 column (H form, 150 ml). After washing with 300 ml of water, elution was effected with 0.5 N aqueous ammonia. The eluate was evaporated under reduced pressure, and the residue was dissolved in 30 ml of water. The solution was extracted with n-butanol (100 ml x 3), the n-butanol was then distilled off under reduced pressure, and the resulting crystals were collected and recrystallized from n-propanol-ethyl ether to give 0.90 g of the title compound. Melting point: 196-1970C 21 (c=0.51, water) [a] D Elemental analysis Calcd. for C15H22N2O4: C, 61.20; H, 7.53; N, 9.52% Found: C, 61.04; H, 7.71; N, 9.51% Example 7 Production of [(R)-3-carboxy-2-carbamoylaminopropyl]- trimethylammonium chloride: Potassium cyanide (364 mg) was added to a solution of 700 mg of deacetyl-FA-5859 dihydrochloride in 20 ml of water. The mixture was allowed to stand at room temperature for 12 hours. The reaction mixture was chromatographed on a Dowex 50 W x 2 column (H+ form, 80 ml), followed by washing with 240 ml of water and elution with 0.5 N aqueous ammonia. The eluate was evaporated under reduced pressure, the residue was dissolved in 3 ml of 1 N HC1 and the water was distilled off under reduced pressure. The oil thus obtained was crystallized from MeOH-ethyl ether and recrystallized from MeOH-EtOH to give 0.53 g of the title compound. Melting point: 215-217"C (decomposition) Ia] D25 24.1" (c=l, water) Elemental analysis Calcd. for C8H18N303Cl C, 40.07; H, 7.56; N, 17.52; C1, 14.80% Found: C, 40.04; H, 7.55; N, 17.14; C1, 14.87% Example 8 Production of [(R) -2-ammonia-3-carbomethoxypropyl] - trimethylammonium dipicrate: To a suspension of 1.0 g of deacetyl-FA-5859 dihydrochloride in 20 ml of MeOH, there was added 5 ml of 6 N NC- MeOH, and the mixture was allowed to stand at room temperature for 2 days. The MeOH was then distilled off under reduced pressure, the residue was dissolved in 50 ml of MeOH, a solution of 2.0 g of picric acid in 50 ml of ethanol was added, and the resulting crystalline precipitate was collected by filtration. Recrystallization from MeOH gave 1.60 g of the title dipicrate. Melting point: 139-1400C 1z1D4 - 70 (C=1.0, dimethylformamide) Elemental analysis Calcd. for C20H24N8 16 C, 37.98; H, 3.82; N, 17.71% Found: C, 37.81; H, 4.04; N, 17.45% Example 9 Production of [(R) -2-ammonio-3-carboethoxypropyli trimethyl- ammonium monochloride monopicrate: EtOH (10 ml) was cooled to -200C and 0.4 ml of thionyl chloride was added dropwise, followed by addition of 0.92 g of deacetyl-FA-5859 dihydrochloride. The mixture was stirred at 0 C for an hour and then at 600C for 8 hours. After removal of the EtOH by distillation under reduced pressure, the residue was dissolved in 10 ml of EtOH and a solution of 2.0 g of picric acid in 50 ml of EtOH was added. The resulting crystalline precipitate was collected by filtration and recrystallized from MeOH-EtOH to give 1.20 g of the title compound. Melting point: -204-208"C lulD5- 2.8 (c=l.l, dimethylformamide) Elemental analysis Calcd. for C15H24N50gC1: C, 39.69; H, 5.32; N, 15.43; Cl, 7.82% Found: C, 39.44; H, 4.90; N, 15.43; C1, 7.09% Example 1Q Production of [(R)-2-ammonio-3-carbopropoxypropyl]- trimethylammonium dipicrate: N-Propanol (20 ml) was cooled to -200C and 0.4 ml of thionyl chloride was added dropwise, followed by addition of 0.70 g of eacetyl-FA-5859 dihydrochloride. The mixture was stirred at 0 C for an hour and then at 600C for 24 hours. After removal of the n-propanol by distillation under reduced pressure, the residue was dissolved in 10 ml of n-propanol and a solution of 0.9 g of picric acid in 20 ml of EtOH was added. The resulting crystalline precipitate was collected by filtration and recrystallized from MeOH-EtOH to give 1.0 g of the title dipicrate. Melting point: 109-llloC [a] 25=0.40 (c=0.93, dimethylformamide) D Elemental analysis Calcd. for C22H28N8O16: C, 40.00; H, 4.27; N, 16.96% Found: C, 39.73; H, 4.32; N, 16.76% Example 11 Production of [(R)-2-ammonio-3-carbamoylpropyljtrimethyl- ammonium dipicrate: MeOH (20 ml) was cooled to -200C and 0.4 ml of thionyl chloride was added dropwise with stirring, followed by addition of 1.30 g of deacetyl-FA-5859 dihydrochloride. The mixture was stirred at OOC for an hour and then at 600C for 3 hours. After removal of the MeOH by distillation under reduced pressure, the residue was dissolved in 10 ml of MeOH and a solution of 2.0 g of picric acid in 30 ml of MeOH was added. The resulting crystalline precipitate was collected by filtration to give crystals (2.20 g), which was recrystallized from MeOH, giving 1.80 g of t(R)-2-ammonio-3-carbomethoxypropyl3trimethylammonium dipicrate. This product (1.0 g) was dissolved in 20 ml of MeOH, and 10 ml of 15 N ammonia water was added. The mixture was allowed to stand at room temperature for 2 days and evaporated under reduced pressure. To the residue was added EtOH and the resulting crystalline precipitate was collected by filtration and recrystallized from MeOHwater to give 0.65 g of the title compound. Melting point: 226-2270C [a] 25 +2.9 (c=1.0, dimethylformamide) D Elemental analysis Calcd. for C19H23N9015: C, 36.96; H, 3.75; N, 20.42% Found: C, 36.99; H, 3.73; N, 20.448 Example 12 Production of (R)-3-myristoylamino-4-trimethylammoniobutyrate: Using 466 g of deacetyl-FA-5859 dihydrochloride and 520 mg of myristoyl chloride and following the procedure of Example 4 for the production of the palmitoyl compound, there was obtained 110 mg of the title compound. 1H-NMR (in CD30D)d ppm: 0.85(3H, t, -CH3), 1.27(20H, m, -(CH2)10-CH3), 3.15(9H, s, (CH3)3N+-), 3.52(2H, d, -CH2-+N(CH3)3) Example 13 Production of [(R)-2-caprylamino-3-carbomethoxypropyl]- trimethylammonium chloride: [(R)-2-Ammonio-3-carbomethoxypropyl]trimethyl- ammonium dipicrate (500 mg) as obtained in Example 8 was dissolved in dimethylformamide (4 ml), and triethylamine (0.6 ml) and a solution of capryl chloride (528 mg) in dimethylformamide (4 ml) were serially added to the solution. The mixture was stirred at room temperature for 3 hours and the solvent was then distilled off under reduced pressure. To the residue was added water (50 ml) and the mixture was adjusted to pH 2 with 1 N hydrochloric acid and extracted with ether (30 mlxS). The ether layers were combined and extracted with water (100 ml x 3). The aqueous layer was adjusted to pH 11 with I N sodium hydroxide and extracted with ethyl acetate-ether (1:1) (150 ml x 2). The extract was washed with water and the solvent was distilled off. The residue was dissolved in 20% methanol-water (50 ml) and passed through an IBA-402 column (C1 form; 5 ml). The effluent and 20% methanolwater washings (20 ml) were combined and lyophilized to give a hygroscopic powder (40 mg). IR #KBr cm-1: 2930, 2860, 1750, 1665 max H-NMRdppm(in heavy water): 0.8-8(3H, t, CH3-(CH2)6-), 1.30(12H, CH3-(cH2)6-), 1.63(2H, m, -(CH2)6-CH2-), 2.30(2H, t, -CH2-COIQH-), 2.81(2H, Cii2-COOCn3) , 3.27(9H, s, (CH3)3N-), 3.73(3H, 5, -COOCH3) Example 14 Production of (R)-3-benzoylamino-4-trimethylammoniobutyrate: To a solution of 700 mg of deacetyl-FA-5859 dihydrochloride in 20 ml of water were added 20 ml of acetonitrile, 1.0 g of benzoic anhydride and 1.1 g of NaHCO3, and the mixture was stirred at 0 C for an hour and then at room temperature for 5 hours. The solvent was then distilled off and the residue was acidified with 1 N aqueous hydrochloric acid and washed with ether. The aqueous layer was passed through a Dawex 50 Wx2 column (H+ form; 80 ml). After washing the column with 200 ml of water, elution was performed with 0.5 N aqueous ammonia. The eluate was evaporated under reduced pressure and the residue was dissolved in water, followed by lyophilization to give 450 mg of the title compound. 1H-NMR 6 ppm(in heavy water): 2.65(2H, d, -CH2-COO ), 3.24(9H, s, (CH3)N+-), 3.70(2H, q, (CH ) + CH,-), 7.70(5H, m, EMI39.1 Example 15 Production of [(R)-2-benzoylamino-3-carbomethoxypropyl trimethylammonium chloride: MeOH (10 ml) was cooled to -200C and, with stirring, 0.15 ml of thionyl chloride was added. To the mixture was added 300 mg of (R)-benzoylamino-4-trimethylammoniobutyrate as obtained in Example 14, and the whole mixture was stirred at OOC for an hour and then at room temperature for 10 hours. After removal of the solvent by distillation, the residue was dissolved in 50 ml of 10% MeOH-water and the solution was passed through an Amberlite IRA-402 column (Cl form; 5 ml). The effluent and 10E MeOH washings were combined and evaporated under reduced pressure, and the residue was dissolved in water, followed by lyophilization to give 250 mg of the title compound as a hygroscopic powder. 1H-NMR a ppm(in heavy water): 2.86(2H, d, -CH2-COOCH3), 3.25(9H, s, (CH3)N+-), 3.72(3H, s, -COOCH3), 7.70(5H, m, EMI40.1 Example 16 Production of (S) -2-acetylamino-3-carboxypropyl) trimethyl- ammonium chloride: (1) Production of Z-D-A2Pr-OH In a mixture of dimethylformamide and water (50 ml 50 ml) was dissolved 12.9 g of bistrifluoroacetoxyphenyliodine and the solution was stirred vigorously. After 15 minutes, 3.2 ml of pyridine was added and the mixture was stirred at room temperature for 4 hours. The solvent was then distilled off under reduced pressure and the resulting crystalline precipitate was collected by filtration. Recrystallization from EtOH gave 3.5 g of Z-D-A2pr-OH. Melting point: 227-2280C lay 24 +8.80 (c=0.4, 1 N NaOH) D Elemental analysis Calcd. for C 4N2 4 C, 55.44; H, 5.92; N, 11.76t Found: C, 54.96; H, 5.76; N, 11.30t (2) Production of Z-D-A2pr(POC)-OH In a mixture of water and acetonitrile was suspended 3.3 g of Z-D-A2pr-OH and the suspension was cooled to 0oC, followed by dropwise addition of 4 ml of triethylamine. To the mixture was added 4.1 g of 2-t-butoxycarbonyloxy- imino-2-phenylacetonitrile (BOC-ON), and the whole mixture was stirred at room temperature overnight. The solvent was then distilled off and the residue was washed with 100 ml of ether. The aqueous layer was acidified with 10% aqueous citric acid and extracted with 100 ml of AcOEt. The extract was washed with water and dried over anhydrous Na2SO4. The AcOEt was distilled off and the residue was crystallized from petroleum ether. Yield 2.90 g. Melting point: 142-143"C 25 [a]D5 +11.0 (c=0.5, methanol) Elemental analysis Calcd. for C16H22N2O6: C, 56.79; H, 6.55; N, 8.288 Found: C, 56.77; H, 6.66; N, 8.04% (3) Production of Z-D-A2bu(BOC)-OMe A solution of 2.80 g of Z-D-A2pr(BOC)-OH and 0.92 ml of N-methylmorpholine in 60 ml of AcOEt was cooled to -100C, and 1.10 ml of ethyl chlorocarbonate was added. The mixture was stirred at OOC for 30 minutes. The insoluble matter was filtered off and a diazomethaneethyl ether solution was added in excess to the filtrate. The mixture was stirred at OOC for an hour and then at room temperature overnight. The solvent was then distilled off under reduced pressure and the residue was dissolved in 50 ml of MeOH, followed by addition of a solution of 100 mg of silver benzoate in 1 ml of triethylamine. The mixture was stirred in the dark for 4 hours. The Insoluble matter was filtered off and the filtrate was concentrated under reduced pressure. The residue was extracted with 200 ml of AcOEt. The AcOEt extract was washed with 10% aqueous citric acid, 5% aqueous NaHCO3 and water in that order and dried over anhydrous Na2SO4. The AcOEt was distilled off and the resulting crystalline precipitate was collected by filtration and recrystallized from AcOEtpetroleum benzine. Yield 1.50 g. Melting point: 101-1030C it324 -5.6 (c=0.9, dimethylformamide) D Elemental analysis Calcd. for C18H26N206: C, 59.00; H, 7.15; N, 7.658 Found C, 59.13; H, 7.06; N, 7.60% (4) Production of Z-D-A2bu(BOC)-OH A solution of 1.45 g of Z-D-A2bu(BOC)-OMe in 30 ml of MeOH was cooled to OOC and 6 ml of 1 N NaOH was added dropwise to the solution. The mixture was stirred at room temperature for 2 hours and the MeOH was distilled off under reduced pressure. The residue was acidified with 10% aqueous citric acid and extracted with 100 ml of AcOEt. The extract was washed with water and dried over anhydrous Na2SO4. The AcOEt was then distilled off and petroleum benzine was added to the residue. The crystals thus obtained were recrystallized from AcOEt-petroleum benzine. Yield 1.15 g. Melting point: 136-1370C lay24 -12.0 C (c=l.0, dimethylformamide) D Elemental analysis Calcd. for C17Hz4N206: C, 57.94; H, 6.87; N, 7.958 Found: C, 58.25; H, 6.75; N, 7.78% (5) Production of Ac-D-A2bu (BOC) -OH In 10% MeOH-water (v/v) was dissolved 1.10 g of Z-D-A2-bu (BOC) -OH and catalytic reduction was carried out in the presence of palladium black. The catalyst was then filtered off and the filtrate was concentrated under reduced pressure. The residue was dissolved in a waterdioxane (20 ml-20 ml) mixture, and 0.80 ml of triethylamine and 0.80 g of N-acetyloxy-5-norbornene-2,3-dicarboximide were added. The mixture was stirred at room temperature for 5 hours. The dioxane was distilled off and the residue was washed with 50 ml of AcOEt. The aqueous layer was acidified with 10% aqueous citric acid and extracted with AcOEt 1100 ml x 2). The AcOEt layer was washed with 5% aqueous sodium chloride and dried over anhydrous Na2SO4. The AcOEt was then distilled off and the residue was crystallized from petroleum benzine, followed by recrystallization from AcOEt. Yield 0.57 g. Melting point: 139-1400C 24 24 D -25.40 (c=0.80, dimethylformamide) Elemental analysis Calcd. for C11H20N2O5: C, 50.75; H, 7.75; N, 10.76% Found: C, 51.11; H, 7.43; N, 10.79% (6) Production of [(S)-2-acetylamino-3-carboxypropyl] trimethylammonium chloride In 20 ml of trifluoroacetic aicd was dissolved 0.53 g of Ac-D-A2bu(BOC)-OH and the solution was allowed to stand at room temperature for 15 minutes. The trifluoroacetic acid was distilled off. Upon treatment with ether, the residue turned into a powder. This powder was dissolved in 8 ml of 8% aqueous NaOH and the solution was cooled to OOC, followed by dropwise addition of 0.5 ml of dimethyl sulfate. The mixture was stirred vigorously at 0 C for 30 minutes and then at room temperature for 3 hours, diluted with 100 ml of water and passed through a Dowex 50 W x 2 column (H+ form, 100 ml). After washing the column with 300 ml of water, solution was carried out with 0.5 N aqueous ammonia. The eluate was evaporated under reduced pressure to remove the solvent and the residue was dissolved in 3 ml of 1 N HC1, followed by concentration under reduced pressure. The resulting oily product was crystallized from MeOH-ethyl ether to give 415 mg of the title compound. Melting point: 218-220"C 24 [aJ2g +20.50 (c=0.92, water) Elemental analysis Calcd. for C9H192O 3C1:: C, 45.28; H, 8.02; N, 11.73; C1, 14.85E Found: C, 45.20; H, 8.10; N, 11.47; C1, 14.85t Example 17 Production of [(S)-2-ammonio-3-carboxypropyl]trimethyl ammonium dichloride: l(S)-Z-Acetylamino-3-carboxypropylZtrimethyl- ammonium chloride (205 mg) as obtained in Example 16 was dissolved in 10 ml of 5.5 N HC1 and treated with at 1000C for 10 hours. The reaction mixture was concentrated under reduced pressure and the residue was crystallized from MeOH-ethyl ether. Yield 173 mg. Melting point: 214-2160C (decomposition) [a124 -8.2 (c=0.58, water) D Elemental analysis Calcd. for C7H18N202C12 C, 36.05; H, 7.77; N, 12.01; C1, 30.408 Found: C, 35.94; H, 7.61; N, 12.14; C1, 28.92% Example 18 (1) The lyophilizate (8.0 g) obtained in the manner as Reference Examples 1 and 2 was recrystallized twice from ethyl alcohol-ethyl ether. The mother liquor was evaporated under reduced pressure and the resulting oil was subjected to column chromatography using Dowex 50 W x 8 (100-200 mesh, 400 ml). Elution was carried out with 0.05 M citric acid buffer and the 5.7-6.2 Q eluate fractions were combined and passed through a Dowex 50 W x 2 column 1100 ml, H form). After washing the column with 300 ml of water, elution was carried out with 0.5 N aqueous amrronia, The eluate was concentrated and the resulting oil was dissolved in methanol, followed by addition of a 5% picric acid-ethanol solution. The resulting crystalline precipitate was collected by filtration and recrystallized from methanol to give 210 mg of [(R)3-carboxy-2-propylaminopropionyl]trimethylammonium picrate. Elemental analysis Calcd. for C16H23N5O10: C, 43.15; H, 5.20; N, 15.728 Found: C, 43.32; H, 5.27; N, 15.69% (2) The 6.4-6.8 t eluate fractions obtained in the above Dowex 50 W x 8 (100-200 mesh) column chromatography were combined, desalted with Dowex 50 W x 2 (H+ form) in the same manner as above, and crystallized using picric acid. There was thus obtained 95 mg of [(R)-3-carboxy-2-butyrylaminopropyl]trimethylammonium picrate. Elemental analysis Calcd. for C17H25N5O10: C, 44.45; H, 5.48; N, 15.248 Found: C, 44.38; H, 5.43; N, 15.30% Example 19 Production of [(R)-2-methylammonio-3-carboxyprcpyl]- trimethylammonium . dichloride: (1) Production of (R)-3-(N-acetyl-N-methylamino)-4trimethylammoniobutyrate: In 40 ml of dimethylformamide were dissolved 1.9 g of FA-5859 hydrochloride obtained in the manner of Reference Example 3 and 7.0 g of silver oxide; and then 4.0 ml of methyl iodide was added to the mixture under stirring. The reaction mixture was stirred at 45QC for 5 hours, and the insolubles were removed by filtration. Dimethylformamide was removed by evaporation under reduced pressure, and to the residue were added 20 ml of methanol and 30 ml of 1N aqueous sodium chloride. Insolubles were removed by filtration and the filtrate was stirred for 2 hours at room temperature. After methanol was removed by evaporation, the residue was dissolved in 100 ml of water and the solution was passed through a Dowex 50 W x 2 column (r. form; 100 ml). After washing the column with 3O0 mi c- ;ater, elution was performed with 400 ml of 0.5N-aqueous ammonia. The eluate was subjected to evaporation to remove the solvent, and the oily residue, which was 1.6 g, was dissolved in 20 ml of water. The solution was passed through a C:4- Sephadex C-25 (Pharmacia Fine Chemicals, Sweden) column (1 Q), which was previously equilibrated with 0.05 M acetate buffer (pH 4.4). Elution was carried out with a 0.05 05 acetate buffer solution and eluate fractions of 1400 ml to 1600 ml were collected and the eluate was passed through Dowex 50 W x 2 column (H form; 100 ml). After the column was washed with 300 ml of water, and elution was carried out with 400 ml of 0.5 N-aqueous ammonia. The eluate was subjected to evaporation to remove the solvent and the residuewas lyophilized, whereupon 0.80 g of the title compound was obtained. H-EMR #ppm (in heavy water): 2.35(3H, s, CH3CO-), 2.68(2H, d, -CH2-COO ), 3.22(3H, s, CH,-N-), 3.36(9H, s, (CH3)3N+-),ca3.8(2H, m, (C113)3N+-CH2), 5.66(1H, m, -CH2-CH-CH2-). (2) Production of [ (R)-2-methylammonio-3-carboxypropyl]- trimethylammonium . dichloride: In 30 ml of 6N-aqueous hydrochloric acid was dissolved 0.50 g of (R)-3-(N-acetyl-N-methylamino)-4-trimethyl- ammoniobutyrate obtained in said item (I), and the solution was treated at 1000C for 24 hours. The reaction mixture was subjected to evaporation under reduced pressure to remove the solvent, and to the residue was added 30 ml of water, and further the mixture was subjected to evaporation under reduced pressure to remove the solvent. After the residue was well dried under reduced pressure, the residue was dissolved in a small amount of water and lyophilized to give 0.45 g of the title compound as oily product. H-M1R oppm (in heavy water): 3.12(3H, s, CH3-NH-), 3.39(2H, d, -CH2-COO#), 3.52(9H, s, (CH3)3N#-), 4.18(2H, d, (CH3)3N#-CH2-), 4,45(1H, m, -CH2-CH2-) I- Example 20 Production of [(R)-2-ethylammonio-3-carboxypropyl]- trimethylammonium . dichloride: (1) Production of (R)-3-(N-acetyl-N-ethylamino)-4- trimethy lammoniobutyrate: In 40 ml of dimethylformamide was dissolved 1.9 g of FA-5859 (free form) obtained in the manner of Reference Example 2, and to the mixture were added 6 il of ethyl iodide and 1.4 g of sodium hydride in oil. The reaction mixture was stirred at 750C for 10 hours, and the precipitates were removed by filtration. Dimethylformamide was removed by evaporation under reduced pressure, and to the residue were added 20 ml of methanol and 30 ii of 1N aqueous sodium chloride, and then the mixture was stirred 2 hours at room temperature. After methanol was removed by evaporation, the residue was dissolved in 100 ml of water and the solution was passed through a Dowex 50 W x 2 column (H+ form; 100 ml). After washing the column with 300 ml of water, elution was carreid out with 400 ml of 0.5 N-aqueous ammonia. The eluate was subjected to evaporation to remove the solvent, and the oily residue, which was 1.4 g, was dissolved in 20 ml of water. The solution was passed through a CM-Sephadex C-25 column (1 Q), which was previously equilibrated with 0.05 M acetate buffer (pH 4.4). Elution was carreid out with 0.05 M acetate buffer and eluate fractions of 1450 ml to 1600 ml were collected and the eluate was passed through Dowex 50 W x 2 column (H form; 60 ml). After the column was washed with 200 ml of water, and eluation was carried out with 200 ml of 0.5 N-aqueous ammonia. The eluate was subjected to evaporation to remove the solvent and the residue was liophilized, whereupon 0.41 g of the title compound was obtained. 1H-NMR gppm (in heavy water): 1.48(3H, t, -N-CH2CH3), 2.39(3H, s, -N-COCH3), 2.78(2H, d, 3.35(9H, s, (CH3)3 -), 3.3 - 4.2(4H, m, -N-CH2-CH3, (CH3)3N#-CH2-), 5.40(1H, m, -CH-CH2-COO#) (2) Production of [(R)-2-ethylammonio-3-carboxypropyl]- trimethylammonium . dichloride: In 30 ml of 6N-aqueous hydrochloric acid was dissolved 0.25 g of (R)-3-(N-acetyl-N-ethylamino)-4-trimethylammoniobutyrate obtained in said item (1), and the solution was treated at 1000C for 24 hours. The reaction mixture was subjected to evaporation under reduced pressure, and to the residue was added 20 ml of water, and further the mixture was subjected to evaporation under reduced pressure. After the residue was well dried under reduced pressure, the residue was dissolved in a small amount of water and lyophilized to give 0.23 g of the title compound as oily product. H-NMR #ppm (in heavy eater): 1.48(3H, t, -N-CH2CH3), 3.45(2H, d, -CH2-COO#), 3.54(9H, s, (CH3)3N -), ca. 3.7(2H, m, -N-CH2CH), 4.20(2H, d,(CH3)3N -CH2), 4.45(1H, m, -CH-CH2-COO#) Example 21 Tablets are prepared by a conventional method employing the following ingredients: (R) - 3-benzyloxycarbonylamino-4- trimethylammoniobutyrate 300 mg Corn starch 50 mg Lactose 28 mg Hydroxypropylcellulose-L 20 mg Magnesium stearate 2 mg 400 mg (per tablet) The daily dose of the above tablets for human adults is generally 4 to 8 tablets after each meal (3 times a day). Example 22 Tablets are prepared by a conventional method employing the following ingredients: (R)-3-palmitoylamino-4-trimethyl ammoniobutyrate 300 mg Corn starch 50 mg Lactose 28 mg Hydroxypropylcellulose-L 20 mg Magnesium stearate 2 mg 400 mg (per tablet) The daily dose of the above tablets for human adults is generally 2 to 4 tablets after each meal (3 times a day). Example 23 Tablets are prepared by a conventional method employing the following ingredients: [(R)-2-methylammonio-3-carboxy propyljtrimethylammonium dichloride 300 mg Corn starch 50 mg Lactose 28 mg Hydroxypropylcellulose-L 20 mg Magnesium stearate 2 mg 400 mg (per tablet) The daily dose of the above tablets for human adults is generally 2 to 4 tablets after each meal (3 times a day). Example 24 Tablets are prepared by a conventional method employing the following ingredients: l(S)-2-ammonio-3-carboxypropyl]- trimethylammonium dichloride 300 mg Corn starch 50 mg Lactose 28 mg Hydroxypropylcellulose-L 20 mg Magnesium stearate 2 mg 400 mg (per tablet) The daily dose of the above tablets for human adults is generally 4 to 8 tablets after each meal (3 times a day).

Claims: What we claime is: 1. A compound of the formula: EMI50.1 wherein R1 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue and R2 is a group represented by the formula -OR3 (in which R3 is a hydrogen atom or a substitued or unsubstituted hydrocarbon residue) or by the formula -NH-R4 (in which R4 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue), provided that when R1 is a hydrogen atom or an acetyl group, R2 is not a hydroxy group; or a pharmaceutically acceptable salt thereof. 2. A compound as claimed in Claim 1, wherein R1 s hydrogen, alkyl of one to 6 carbon atoms or acyl of one to 18 carbon atoms, the alkyl and the acyl being unsubstituted or substituted by lower alkoxy, halogen, hydroxy, nitro, amino, cyano, sulfo, aryl or aralkyloxy. 3. A compound as claimed in Claim 1, wherein RL is hydrogen, alkyl of 1 to 6 carbon atoms or acyl of one to 18 carbon atoms. 4. A compound as claimed in Claim 1, wherein R2 is hydroxy, alkoxy of one to 6 carbon atoms or amino. 5. A compound as claimed in Claim 1, where the compound is one in which R1 is myristoyl or palmitoyl and R2 is hydroxy, or one in which R1 is hydrogen and R2 is ethoxy or amino. 6, A method of producing a compound of the formula: EMI51.1 wherein R1 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue and R2 is a group represented by the formula -OR3 (in which R3 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue) or by the formula -NH-R4 (in which R4 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue), provided that when R1 is a hydrogen atom or an acetyl group, R2 is not a hydroxy group; or a salt thereof; which comprises subjecting a compound of the formula: EMI51.2 or a salt thereof to hydrocarbon residue introduction reaction, esterification reaction and/or amidation reaction. 7. A method as claimed in Claim 6, wherein R1 is hydrogen, alkyl of one to 6 carbon atoms or acyl of one to 18 carbon atoms, the alkyl and the acyl being unsubstituted or substituted by lower alkoxy, halogen, hydroxy, nitro, amino, cyano, sulfo, aryl or aralkyloxy; and R2 is hydroxy, alkoxyof one: to 6 carbon atoms or amino. 8. An antidiabetic agent which contains a compound of the formula: EMI52.1 wherein R1 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue and R2 is a group represented by the formula -OR3 (in which R3 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue) or by the formula -NH-R4 (in which R4 is a hydrogen atom or a substituted or unsubstituted hydrocarbon residue), provided that when R1 is a hydrogen atom or an acetyl group, R2 is not a hydroxy group; or a pharmaceutically acceptable salt thereof. 9. An antidiabetic agent as claimed in Claim 8, wherein R1 is hydrogen, alkyl of one to 6 carbon atoms or acyl of one to 18 carbon atoms, the alkyl and the acyl being unsubstituted or substituted by lower alkoxy, halogen, hydroxy, nitro, amino, cyano, sulfo, aryl or aralkyloxy, and R2 is hydroxy, alkoxy of one to 6 carbon atoms or amino. 10. A compound in the optically active D-form which is represented by the formula: EMI53.1 wherein R5 is a hydrogen atom or an acetyl group; or Åa pharmaceutically acceptable salt thereof. 11. A method of producing a compound of the formula: EMI53.2 wherein R6 is -COCH2CH3 or -COCH2CH2CH3, which comprises cultivating a microorganism belonging to the genus Emericella and capable of producing the above compound in a culture medium so as to cause formation and accumulation of the above compound in the culture medium and isolating said compound. 12. A method as claimed in Claim 11, wherein the microorganism is Emericella quardrilineata IFO 5859.

Inventors: ASAI, MITSUKO, HARADA, SETSUO, KANAMARU, TSUNEO, SHINAGAWA, SUSUMU
Applicants: TAKEDA CHEMICAL INDUSTRIES, LTD.
Application year: 1984
Main classification: C07C101
IPC codes: C12P13, C07C275