COMPOUNDS CONTAINING BETA-LACTAMS

Abstract: A compound of formula (I) or a salt or ester thereof: wherein X represents chlorine, bromine, or iodine. Pharmaceuti­ cal compositions and use are also disclosed for the compound of formula (I) and pharmaceutically acceptable salts and esters thereof.

Description: COMPOUNDS CONTAINING '3-LACTAMS This invention relates to a class of novel ss-lactam compounds, to the process for their preparation and to pharmaceutical compositions containing them. U.K. Patent Nos. 1,508,977 and 1,508,978 disclose inter alia clavulanic acid and its salts and esters. Clavulanic acid has the formula (A): EMI1.1 Ethers of clavulanic acid, its salts and esters are disclosed in U.K. patent specification No. 1 565 209 which are of formula (B): EMI1.2 wherein R is an inert organic group of up to 18 carbon atoms and A is a group such that CO2A represents a carboxylic acid group or a salt or ester thereof. The compounds of both formula (A) and formula (B) possess anti-bacterial and ss-lactamase inhibitory activity. We have now found a novel class of halogen containing compounds which not only have anti-bacterial and ss-lactamase inhibitory activity but also have the advantage of exhibiting high and prolonged blood levels upon administration. Accordingly, the present invention provides a compound of formula (I) or a salt or ester thereof: EMI2.1 wherein X represents chlorine, bromine, or iodine. Preferably X is chlorine. The major utility of the compound of formula (I) is as a pharmaceutical and, accordingly, the salts and esters of the compound of formula (I) are preferably pharmaceutically acceptable. The compound of formula (I) may also be used as an anti-bacterial or ss-lactamase inhibitor in non-pharmaceutical uses such as, for example, as a disinfectant or paint additive; those salts and esters which are not normally considered to be pharmaceutically acceptable are suitable for this application. Suitable pharmaceutically acceptable salts of the compounds of formula (I) include metal salts, eg aluminium, alkali metal salts such as sodium or potassium, alkaline earth metal salts such as calcium or magnesium and ammonium or substituted ammonium salts, for example those with lower alkylamines such as triethylamine, cycloalkylamines such as bicyclohexylamine, or with dibenzylamine N,N-dibenzylethylenediamine, l-ephenamine, N-ethylpiperidine, or N-benzyl-ss- phenethylamine. Particularly suitable pharmaceutically acceptable salts of the compound of formula (I) are the alkali metal salts such as lithium, sodium and potassium. The preferred pharmaceutically acceptable salts are the sodium and potassium salts. Suitable pharmaceutically acceptable esters of the compounds of formula (I) include in vivo hydrolysable esters. Further suitable pharmaceutically acceptable esters include alkyl esters, such as for example C1-6 alkyl esters. Examples of suitable pharmaceutically acceptable in vivo hydrolysable ester groups include those which break down readily in the human body to leave the parent acid or its salt. Suitable ester groups of this type include those of part formula (i), (ii) and (iii): EMI3.1 -CO2CH20Rf (iii) wherein Ra is hydrogen, methyl, or phenyl, Rb is C16 alkyl, C16 alkoxy or phenyl; or Ra and Rb together form a 1,2-phenylene group optionally substituted by one or two methoxy groups; Re represents C1-6 alkylene optionally substituted with a methyl or ethyl group Rd and Re independently repent C16 alkyl; Rf represents C1-6 alkyl. Examples of suitable in vivo hydrolysable ester group include for example acyloxyalkyl groups such as acetoxymethyl, pivaloyloxymethyl, ck-acetoxyethyl and i-pivaloyloxyethyl groups; alkoxycarbonyloxyalkyl groups, such as ethoxycarbonyl-oxymethyl and ckethoxycarbonyloxyethyl; dialkylamino-alkyl especially di-loweralkylamino alkyl groups such as dimethylaminomethyl, dimethylaminoethyl, diethylaminomethyl or diethylaminoethyl; lactone groups such as phthalidyl and dimethoxyphthalidyl; and esters linked to a second -lactam antibiotic or to a '3-lactamase inhibitor. The present invention also provides a pharmaceutical composition which comprises a compound of this invention and a pharmaceutically acceptable carrier. The compositions of the invention include those in a form adapted for oral, topical or parenteral use and may be used for the treatment of the infection in animals especially mammals including humans. Suitable forms of the compositions of this invention include tablets, capsules, creams, syrups, suspensions, solutions, reconstitutable powders and sterile forms suitable for injection or infusion. Such compositions may contain conventional pharmaceutically acceptable materials such as diluents, binders, colours, flavours, preservatives, disintegrant and the like in accordance with conventional pharmaceutical practice in the manner well understood by those skilled in the art of formulating antibiotics. Injectable or infusable compositions of a compound of the invention are particularly suitable as high blood levels of the compound can occur after administration by injection or infusion. Thus, one preferred composition aspect of this invention comprises a compound of the invention in sterile form and most suitably in sterile crystalline form. The injectable solution of the compound of this invention may be made up in a sterile pyrogen-free liquid such as water, aqueous ethanol or the like. An alternative approach to administering the compounds of this invention is to utilise an injectable suspension. Such suspensions may be made up in sterile water; sterile saline or the like and may also contain suspending agents such as polyvinylpyrrolidone, lecithin or the like. Alternatively such compositions may be prepared in an acceptable oil suspending agent such as arachis oil or its equivalent. For use in such suspensions the compounds of this invention should be in the form of fine particles. Unit dose compositions comprising a compound of this invention adapted for oral administration form a further sutiable compsotion aspect of this invention. Unit dose compositions comprising a compound of this invention adapted for topical administration are çalso presented by this invention. In this instance 'topical administration' also includes local Rdministration to internal surfaces of mammary glands of cattle, for example during the treatment of mastitis by intra-mammary administration. The compound of the formula may be present in the composition as sole therapeutic agent or it may be present together with other therapeutic agents such as, a penicillin or cephalosporin. Considerable advantages accrue from the inclusion of a penicillin or cephalosporin which shows instability to ss-lactamases since the resulting composition shows enhanced effectiveness (synergy). Suitable penicillins, cephalosporins or other P-lactam antibiotics for inclusion in such synergistic compositions include not only those known to be highly susceptible to ss-lactamases but also those which have a degree of intrinsic resistance to ss-lactamases. Suitable penicillins for inclusion in the compositions of this invention include benzylpenicillin, phenoxymethylpenicillin, carbenicillin, azidocillin, propicillin, ampicillin, amoxycillin, epicillin, ticarcillin, cyclacillin, pirbenicillin, azlocillin, mezlocillin, sulbenicillin, piperacillin, and other well known penicillins including pro-drugs thereof such as their in vivo hydrolysis esters such as the acetoxymethyl, pivaloyloxymethyl, a-ethoxycarbonyloxyethyl or phthalidyl esters of ampicillin benzylpenicillin or amoxycillin, and aldehyde or ketone adducts of penicillins containing a 6- -aminoacetamide side chain (such as hetacillin, metampicillin and analogous derivatives of amoxycillin) or esters of carbenicillin or ticarcillin such as their phenyl or indanyl a-esters. Suitable cephalosporins for inclusion in the compositions of this invention include, for example, cefatrizine, cephaloridine, cephalothin, cefazolin, cephalexin, cephacetrile, cephapirin, cephamandole nafate, cephradine, 4-hydroxycephalexin, cephaloglycin, cefoperazone, and known cephalosporins or pro-drugs thereof. Such compounds are frequently used in the form of a salt or hydrate or the like. Naturally if the penicillin or cephalosporin present in the composition is not suitable for oral administration then the composition will be adapted for parenteral administration. Highly favoured penicillins for use in the compositions of this invention include ampicillin, amoxycillin, carbenicillin and ticarcillin. Such penicillins may be used as a pharmaceutically acceptable salt such as the sodium salt. Alternatively the ampicillin or amoxycillin may be used in the form of fine particles of the zwitterionic form (generally as ampicillin trihydrate or amoxycillin trihydrate) for use in an injectable suspension, for example, in the manner hereinbefore described for a compound of this invention. The preferred penicillin for use in the synergistic composition is amoxycillin, for example as its sodium salt or trihydrate. Particularly suitable cephalosporins for use in the compositions of this invention include cephaloridine and cefazolin which may be in the form of a pharmaceutically acceptable salt, for example the sodium salt. When present together with a cephalosporin or penicillin, the ratio of a compound of the invention to the penicillin or cephalosporin agent may vary over a wide range of ratios, such as from 10:1 to 1:10, for example about 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5 or 1:6 (wt/wt, based on pure free antibiotic equivalent). Orally administrable compositions containg a compound of the invention will normally contain relatively more synergist than corresponding injectable compositions. The total quantity of a compound of the invention in any unit dosage form will normally be between 25 and 1000 mg and will usually be between 50 and 500 mg, for example about 62.5, 100, 125, 150, 200 or 250 mg. Compositions of this invention may be used for the treatment of infections of inter alia, the respiratory tract, the urinary tract and soft tissues in humans and mastitis in cattle. Normally for adult human treatment between 50 and 3000 mg of the compounds of the invention will be administered each day of treatment but more usually between 100 and 1000 mg of the compounds of the invention will be administered per day, for example at 1-6 doses, more usually as 2, 3 or 4 doses. However for the treatment of more severe systemic infections or infections of particularly intransigent organisms higher doses may be used in accordance with clinical practice. The penicillins or cephalosporin in the synergistic composition of this invention will normally be present at approximately the amount at which it is conventionally used which will usually be expected to be from about 62.5 to 3000 mg per dose, more usually about 125, 250, 500 or 1000 mg per dose. One particularly favoured composition of this invention will contain from 150 to 1000 mg of amoxycillin as the trihydrate or sodium salt and from 25 to 500 mg of a compound of this invention. A further particularly favoured composition of this invention will contain from 150 to 1000 mg of ampicillin or a pro-drug thereof and from 25 to 500 mg of a compound of this invention. Most suitably this form of composition will contain ampicillin trihydrate, ampicillin anhydrate, sodium ampicillin, hetacillin, pivampicillin, hydrochloride, bacampicillin hydrochloride or talampicillin hydrochloride. Most suitably this form of the composition will contain a compound of the formula (I) when in crystalline form. Most suitably the preceding composition will contain from 200 to 700 mg of the penicillin component. Most suitably the preceding composition will comprise from 50 to 250 mg of a compound of the formula (I) preferably in crystalline form. Such compositions may be adapted for oral or parenteral use except when containing an in vivo hydrolysable ester of ampicillin or amoxycillin in which case the compositions will not be adapted for parenteral administration. Another particularly favoured compositions of this invention will contain from 200 to 2000 mg of carbenicillin, ticarcillin or a pro-drug thereof and from 50 to 500 mg of a compound of this invention. Suitably this form of composition will contain di-sodium carbenicillin. Suitably this form of the composition will contain di-sodium ticarcillin. More suitably this form of the composition will contain from 75 to 250 mg of a compound of the formula (I) preferably in crystalline form. Such compositions containing di-salts of carbenicillin and ticarcillin will be adapted for parenteral administration. The present invention also provides a method of treating bacterial infections in humans or animals including domestic mammals which comprises the administration of a composition of this invention Commonly the infection treated will be due to a strain of Staphylococcus aureus, Klebsiella aerogenes, Escherichia coli, Proteus sp., Bacteroides fragilis or the like. The organisms bel eved to be most readily treated by an antibacterially effective amount of a compound of this invention is Staphylococcus aureus. The other organisms named are more readily treated by using a synergistically effective amount of the compound of this invention and a penicillin or cephalosporin. The administration of the two components may take place separately but in general we prefer to use a composition containing both the synergist and the penicillin or cephalosporin. The indications for treatment include respiratory tract and urinary tract infections in humans and mastitis in cattle. The present invention also provides a process for the preparation of a compound of formula (I) or a salt or ester thereof, which process comprises halogenation (i.e. chlorination, bromination or iodination) of a compound of formula (II): EMI10.1 wherein Rx is a carboxy protecting group; and thereafter where necessary carrying out one or more of the following steps: (a) removing the carboxy protecting group RX; (b) converting a salt to the free carboxylic acid or to an ester, or to a different salt. The halogenation may be achieved by using halogenating reagents such as thionyl halide, e.g. thionyl chloride,oxyhalides such as phosphorous oxychloride, oxalylhalide, fcr example oxalylchloride. Chlorination may also be achieved by reaction of a compound of formula (II) with methane sulphonyl chloride or toluene sulphonyl chloride in dimethyl formamide. The chlorination is suitably carried out in the presence of an organic base such as pyridine. The preceding reaction normally takes place in hexamethylphosphoramide. The reaction is generally carried out at a ambient or elevated temperature for example +200C to +8O0C, and preferably at an elevated temperature, for example +3O0C to +5O0C. The compound of formula (II) may itself be prepared by a method described in European patent publication No. 0002370. Suitable carboxy protecting groups for the group -CO2RX in formula (II) include ester derivatives of the carboxylic acid. The derivative is preferably one which may readily be cleaved at a later stage of the reaction. Suitable ester-forming carboxylic-blocking groups are those which may be removed under conventional conditions. Such groups for Rx include benzyl, p-methoxybenzyl, 2,4,6-trimethylbenzyl, 3,5-di-t-butylbenzyl, 4-pyridylmethyl, allyl, diphenylmethyl, triphenymethyl, 2-benzyloxphenyl, 4-methylthiophenyl, methoxymethyl, a silyl, or phosphorus-V-containing group, or methyl or ethyl or an in vivo hydrolysable ester as hereinbefore defined. The carboxylic group or a salt thereof may be regenerated from any of the above esters by usual methods appropriate to the particular Rx group, for example, base-catalysed hydrolysis, or by enzymicallycatalysed hydrolysis, or by hydrogenation. Acids within formula (I) may also be prepared by the careful acidification of a corresponding salt such as the sodium salt. Salts within formula (I) may be prepared by treatment of an acid within formula (I) with, for example, sodium ethyl hexanoate or potassium ethyl hexanoate. Salts within formula (I) may also be prepared by salt exchange in conventional manner, for example a solution of the lithium salt in water may be passed through a bed or ion exchange resin in the sodium form (e.g. Amberlite*) 120; a sodium salt of a sulphonate polystyrene divinyl benzene co-polymer) in about ten-fold excess until elution is complete; the resulting sodium salt may be obtained by freeze drying or by evaporation. Similarly, a sodium salt may be converted to a lithium salt or to a potassium salt in similar manner. (Amberlite is a trade name.) The following Examples illustrate the invention. In the Examples temperatures are given in OC. Preparation 1 p-Nitrobenzyl-9-0-(2-hydroxyethyl ) clavulanate A solution of p-nitrobenzyl clavulanate (30g) and ethylene oxide (6.7g ) in dichloromethane (200ml) was cooled to OoC and treated with boron trifluorideetherate (1.8ml). The mixture was stirred at 0 for t hr. and then at room temperature for i hr. The mixture was then shaken with excess aqueous sodium bicarbonate solution and the organic phase separated. The aqueous phase was thoroughly extracted with chloroform and the combined organic phases washed with brine, dried over anhydrous magnesium sulphate and evaporated. Chromatography on silica gel using ethyl acetate-petrol 1:1 gave the title compound as a crystalline compound (5.08 g; 15%) m.p. 69-70 (ethylacetate-petrol); [aZ20 + 40.60 (C. 1.0, CHC13); vmax (CHC13) 3450, 1805, 1760, 1695 and l605cm1; 6(CDC13) 2.07 (1H, broads, -OH), 3.06 (1H, d, J=17Hz, 6ss-CH), 3.35-3.85 (5H, m), 4.12 (2H, d, J=7Hz, 9-CH2), 4.88 (1H, t, J=7Hz, 8-CH), 5.16 (1H, s, 3-CH), 5.32 (2H, s, Ar-C), 5.71 (1H, d, J=3Hz, 5-CH), 7.55 and 8.29 (4H, 2d, Ar-H). Example 1 p-Nitrobenzyl-9-0- (2-chloroethyl) clavulanate p-Nitrobenzyl-9-0-(2-hydroxyethyl)clavulanate (0.83g) was dissolved in dimethylformamide (20ml) and the solution warmed to 600. At this temperature methanesulphonyl chloride (0.5g) and pyridine (0.35g) were added and the mixture stirred at 600 for i hr. The solution was allowed to cool and diluted with excess ethyl acetate. The organic solution was washed successively with water, dilute hydrochloric acid, sat. aqueous sodium bicarbonate and finally brine. After drying over anhydrous magnesium sulphate the organic solution was evaporated to give an oil. Chromatography on silica gel using ethyl acetate-petrol 1:1 as eluant gave the title compound as a colourless oil (0.161g; 18%), t120 + 36.5 (C.l.0, CHC13); vmax (CHCl3) 1805, 1755, 1695 and 1605 cm¯1; 6(CDC13), 3.06 (1H, d, J=l7Hz, 6-ss-CH), 3.50-3.62 (5H, m), 4.13 (2H, d, J=7Hz, 9-CH2), 4.84 (1H, t, J=7Hz, 8-CH), 5.12 (1H, S, 3-CH), 5.28 (2H, S, Ar-CH2j, 5.68 (lH, dt J=3Hz, 5-CH), 7.5 and 8.23 (4H, 2d, Ar-H). Example 2 Lithium-9-O-[2-chloroethyllclavulanate p-Nitrobenzyl-9-0-(2-chloroethyl)clavulanate (90mg) was dissolved in tetrahydrofuran (10 ml) with stirring, ice-cooled and treated with 1M aqueous ammonium chloride solution (3 ml) and iron powder (0.37g). After 20 minutes a further amount of 1M ammonium chloride solution (1.2ml) and iron powder (0.3g) were added and the stirring continued for 45 minutes. Ethyl acetate (lOOml) was added and H2S bubbled through the mixture for 10 minutes with ice-cooling. The mixture was filtered through Celite and the residues washed with water (20 ml). The aqueous layer of the filtrate (including washings) was saturated with Nail, acidified with 1N hydrochloric acid to pH 2.5 and separated from the organic layer. The aqueous layer was further extracted with ethyl acetate (2 x 30m1) and the combined ethyl actate extracts dried over magnesium sulphate. After filtration the ethyl acetate was extracted with pH7 phosphate buffer. The combined aqueous extracts were saturated with NaCl acidified to pH 2.5 with 1N hydrochloric acid and extracted with ethyl acetate. The ethyl acetate extracts were dried over Mg SO4 and evaporated and the residue quickly taken up in tetrahydrofuran (10 ml) and water (5 ml). This solution was brought to pH7 by addition of lithium hydroxide solution, washed with ether and freeze dried to give the title compound (40 mg; 63%). Identical in all respects (ir, nmr, etc.) to the compound described in example 4. Example 3 Benzyl-9-0-12-chloroethylZclavulanate Benzyl-9-0-(2-hydroxyethyl)clavulanate (2.12g) in dimethylformamide was treated at 600 with methanesulphonyl chloride (0.76g) and then pyridine (0.52g). Stirring was continued at this temperature for i hr. and then the solution was poured into an excess of ethyl acetate. The organic solution was washed succesively with water, 3N hydrochloric acid, saturated aqueous sodium bicarbonate and finally brine. After drying over anhydrous magnesium sulphate the solution was evaporated to give an oil. Chromatography on silica gel using ethyl acetate-petrol 1:1 gave the title compound as an oil (0.28g; 13%), [a]20+46.20 [C. 1.0, CHC13], human (CHC13) 1800, 1745 and 1695 cm'l; 6(CDC13), 3.03 (1H, d, J=17Hz, 6ss-CH), 3.5-3.61 (5H, m), 4.12 (2H, d, J=7Hz, 9-CH2), 4.81 (lH, t, J=7Hz, 8-CH), 5.10 (1H, S, 3-CH), 5.18 (2H, s, Ar-CH2), 5.68 (1H, d, J=3Hz, 5-CH), 7.35 (5H, s, Ar-H). The same experiment was conducted using the following ingredients: Benzyl-9-0-2-(2-hydroxyethyl) clavulanate (1.9g; 0.0057m), DMF (30ml), methanesulphonyl chloride (1.3g; 0.0114m), pyridine (0.99; 0.0114m). Benzyl-9-0-2-(2-chloroethyl)-clavulanate (0.570g; 29%) was isolated. Example 4 Lithium 9-0-(2-chloroethyl)clavulanate Benzyl-9-0-[2-chlorgethyl]clavulanate (0.212g) in tetrahydrofuran (10ml) was hydrogenated over 10% palladium-charcoal (0.106g) for ¸ hr. The solution was filtered through celite and the filter-cake washed with tetrahydrofuran. The combined filtrates were diluted with water and a solution of aqueous lithium hydroxide added until pH 7 was attained. The tetrahydrofuran was evaporated and the aqueous solution washed with ether and freeze-dried to give the title compound as a pale beige solid (0.114g; 68%); smax (KBr) 1765, 1690 and 1615cm¯l; 6(D2O), 3.09 (1H, d, J=17Hz, 6ss-CH), 3.44-3.72 (5H, m), 4.17 (2H, d, J=7Hz 9-CH2), 4.89 (1H, t, J=7Hz, 8-CH), 4.96 (1H, s, 3-CH), 5.71 (1H, d, J=3Hz, 5-CH). Example 5 p-Nitrobenzyl-9-0- ( 2-Chloroethyl) clavulanate p-Nitrobenzyl-9-0-(2-hydroxyethyl) clavulanate (lg;0.0026mol) in dry hexamethylphosphoramide (HMPA) (15ml) was treated, undernitrogen, with thionyl chloride (O.256m1;l.2 equivalents) and pyridine (0.18m1;1,2 equivalents). The mixture was stirred at room temperature or 4 hours, poured onto saturated sodium bicarbonate cooled with ice, and extracted with ethyl acetate (2x50ml). The organic layer was washed with dilute hydrochloric acid, water and brine, dried over anhydrous magnesium sulphate and evaporated to give an orange oil. Chromatography of the residue on silica-gel using ethyl acetate-petrol (1:1) as eluant gave the title ester as a colourless oil (0.69g; 67%). Biological Data The compound of Example 4 was administered either orally (by gastric lavage) or subcutaneously as a solution to groups of mice. At intervals after administration, 5 mice which had received the compound subcutaneously and 10 mice which had received the compound orally were sacrificed, and blood collected from the axillary region. The blood was assayed for content of compound 4 using a biological ss-lactamase inhibition-zone assay technique. The mean values (+ standard deviation - SD) are tabulated below. When administered by either an oral or parenteral route, the compound of Example 4 produced high and prolonged levels in the blood of mice. Table 1 Mean blood levels in mice ( g/ml) following oral administration of the compound of Example 4 of the present invention at a dosage rate of 20 mg/kg. EMI18.1 <tb> <SEP> Time <SEP> Time <SEP> after <SEP> administration <SEP> (mins) <tb> <SEP> 15 <SEP> 30 <SEP> 45 <SEP> 60 <SEP> 90 <SEP> 120 <SEP> AUC <tb> average <tb> for <SEP> 10 <SEP> 16.57 <SEP> 10.46 <SEP> <SEP> 7.94 <SEP> 7.42 <SEP> 3.91 <SEP> 3.2 <SEP> 14.28 <tb> mice <tb> +S.D. <SEP> 6.8 <SEP> 2.39 <SEP> 2.83 <SEP> 2.82 <SEP> 1.14 <SEP> 1.27 <tb> Table 2 Mean blood levels in mice ( g/ml) following subcutaneous administration of the compound of Example 4 of the present invention at a dosage rate of 10 mg/kg. EMI19.1 <tb> <SEP> Time <SEP> after <SEP> administration <SEP> (mins) <tb> <SEP> 10 <SEP> 20 <SEP> 30 <SEP> 45 <SEP> 60 <SEP> 90 <SEP> 120 <SEP> AUC <tb> average <tb> <SEP> for <SEP> 5 <SEP> 22.8 <SEP> 17.3 <SEP> 13.3 <SEP> 7.7 <SEP> 5.2 <SEP> 2.7 <SEP> 1.8 <SEP> 15.1 <tb> <SEP> mice <tb> ts.D. <SEP> 3.98 <SEP> 3.6 <SEP> 1.98 <SEP> 0.54 <SEP> 1.08 <SEP> 0.82 <SEP> 1.14 <tb>

Claims: A. CLAIMS 1. A compound of formula (I) or a salt or ester thereof: EMI20.1 wherein X represents chlorine, bromine, or iodine. 2. A compound of formula (I) or a pharmaceutically acceptable salt or pharmaceutically acceptable ester thereof. 3. A compound as claimed in claim 1 or claim 2 wherein X is chlorine. 4. The sodium or potassium salt of a compound of formula (I). 5. The lithium salt of a compound of formula (I). 6. A pharma ceutical composition which comprises a compound as claimed in claim 2 and a pharmaceutically acceptable carrier. 7. A pharmaceutical composition as claimed in claim 6 which further comprises a penicillin or cephalosporin. 8. A process for the preparation of a compound as claimed in claim 1 which process comprises halogenation of a compound of formula (it): EMI21.1 and thereafter where necessary carrying out one or more of the following steps: (a) removing the carboxy protecting group Rx (b) converting a salt to the free carboxylic acid or to an ester, or to a different salt. 9. A compound as claimed in claim 1 for use in treating bacterial infections. 10. The non-pharmaceutical anti-bacterial use of a compound as claimed in claim 1.

Inventors: DAVIES, JOHN SYDNEY, HUNTER, PAMELA ANN
Applicants: BEECHAM GROUP PLC
Application year: 1983
Main classification: C07D498
IPC codes: C07D503