ORAL COMPOSITIONS

Abstract: The invention relates to an oral composition having anti­ caries and anti-plaque activity comprising 0.01 to 3% by weight of stannic ammonium fluoride [Sn(NH₄)₂F₆]

Description: ORAL COMPOSITIONS This invention relates to compositions for the care of the oral cavity. The efficacy of fluorine in the prevention of dental caries is well established (see for example US Patents 3 029 191, 3 070 510 and 3 227 617). The acceptance of fluoride ions as an anticariogenic agent is based essentially on the observation that among the many topical agents that have been tested in clinical trials only those that contain fluorine have been effective in reducing caries. Fluorine-free salts tested have not prevented caries formation. It is known that sodium hexafluorostannate (Na2SnF6) applied topically to the teeth has a caries inhibiting effect (Caries Res. 3, 315-325, 1969). We have now found that stannic ammonium fluoride, Sn(NH4)2F6, (which may also be called ammonium hexafluorostannate),as well as having an anti-caries effect also has a plaque inhibiting effect (which has not been reported for sodium hexafluorostannate) and furthermore that stannic ammonium hexafluorostannate has an important practical advantage over the use of sodium hexafluorostannate in that it has a significally better taste than the sodium compound. Accordingly the invention provides an oral composition having anti-caries and anti-plaque activity comprising 0.01 to 3% by weight of stannic ammonium fluoride The synthesis of Sn(NH4)2F6 is described in J.Am.Chem.Soc. 80, 2662, 1958. In the preparation of therapeutic or prophylactic oral compositions incorporating Sn(NH4)2F6, a suitable carrier or oral media well known in the art is used. Adjuvants such as colouring agents, flavours, humectants, abrasives, detergents, preservatives, emollients and the like and other therapeutic agents compatible with Sn(NH4)2F6 may also be included. The oral composition is preferably in the form of a toothpaste or mouthwash. A particularly suitable amount of the Sn(NH4)2F6 is such as to provide from 1000 to 1500 ppm of fluoride ion. However, the amount may conveniently range from 100 to 3000 ppm. The following Experiments and Examples illustrate the invention. Percentages are by weight. The Sn(NH4)2F6 employed in the following experiments was synthesised according to the method of Haendler and Johnson (J.Am.Chem.Sec. 80, 2662, 1958). The analytical details were as follows: Theoretical % Analytical * Sn 44.18 45.64 NH4 13.41 12.65 F 42.41 43.14 Cariogenic Bacteria used for testing the effect of Sn(NH4)2F6 were Streptococcus mutans (strains coded 6715 and OMZ-176), Streptococcus sanguis (strains coded 10556 and 10557), Streptococcus salivarius (strains coded SS4 and H257), and Lactobacillus casei. All of the above organisms were maintained as lyophilised cultures until the time for use. An aqueous solution of Sn(NH4)2F6 has a pH of about 3.8 as compared to a pH of 3.0 for SnF2. This higher pH characteristic eliminates some of the formulation problems encountered with the strong acid effect of SnF2 The preferred pH of oral compositions containing Sn(NH4)2F6 is from 3.5 to 8.0. A 1000 ppm F solution of Sn(NH4)2F6 was stable. It did not become hazy on standing after several weeks. In contrast, a SnF2 solution developed turbidity, probably due to oxy-fluoride formation, after 24 hours at room temperature. SnF2 at concentrations equivalent to 1000 ppm and 2500 ppm released, respectively,80% and 45% of the available F. Sn(NH4)2F6 displayed the same ionisation values as SnF2. The corresponding values for Na2SnF6 are 73% and 38%, respectively. Thus Na2SnF6 is less ionised than Sn(NH4)2F6. Sn(NH4)2F6 is significantly better tasting than Na2SnF6. A comparative test was carried out with a panel of 3 assessors, and tasting (20 sec. rinse with 10 ml of test solution containing 1000 ppm F ) was performed with coded solutions or on a blind basis. The finding that Sn (NH4)2 F6 had a significantly less metallic taste than Na2SnF6 was the unanimous decision of the three judges. Sn(NH4)2F6 was also much less astringent and metallic tasting than the uncomplexed fluoride salt SnF2 at equal molar concentrations. A similar blind test was conducted to compare the taste of Sn(NH4)2F6 with that of SnF2. This showed that Sn(NH4)2F6 had a significantly less metallic taste than SnF2 and this was the unanimous decision of the three judges. It is generally agreed that oral bacteria are responsible for tooth decay. Therefore, a reduction of the cariogenic flora usually results in a decrease in caries formation. The inhibitory action of the test compounds was. determined by adding each salt, at known concentrations, to a culture of cariogenic bacteria. After 24 hours at 370C bacterial growth was monitored as a change in optical density. A dose response curve (concentration of salt vs. growth) for each compound against each bacterial isolate was plotted in order to obtain the concentration of test agent which would cause a 50% inhibition in growth (ID/50). This is a useful ranking index since it is not always possible to obtain complete inhibition with weak germicidal agents. Only those compounds which were able to elicit an ID/50 response were considered as inhibitory agents. The medium used for bacterial growth experiments was Trypticase Soy Broth (B.B.L.: Baltimore Biological Labs., Baltimore, MD) supplemented with 0.1% Tween 80 (the word Tween is a trade mark) and 0.05% sodium thioglycollate. The test organisms; as lyophilised cells, were grown overnight in 50 mls of growth medium in a vacuum desiccator with an atmosphere of 97% CO2/3% H2 at 350C. These cultures were then used at a 0.2% inoculum to prepare second stage seed flasks. The test media (10ml) consisting of growth medium plus the potential inhibitors, contained in 16 x 120 mm screw top test tubes, were inoculated with the second stage seed cultures to given an absorbancy reading of 0.05. Immediately after inoculation the cultures were incubated for 24 hours under anaerobic conditions. The growth medium was sterilised by autoclaving, whereas the potential inhibitors (as aqueous solutions) were sterilised by filtering each solution separately through a Millipore membrane (0.22 /u). Absorbance at 600 nm versus a medium control was used as a measure of growth on a Beckman Model B spectrophotometer. All absorbancy readings were determined in 16 mm x 120 mm Kimax test tubes. This instrument was linear to 0.60 OD units; thus, all cultures displaying values above this range were diluted with the control, accordingly. TABLE I INHIBITION OF BACTERIAL GROWTH ID/50 (mM/L) S.mutans S.salivarius S.sanguis L.casei 6715 OMZ-176 SS4 H-257 10556 10557 Salts * SnCl2 2.26 3.68 1.45 3.64 1.27 1.88 3-6.0 * SnF 1.50 2.28 2.45 0.74 0.44 0.84 2.97 * Sn(NH4bB6 1.20 1.43 1.65 0.40 0.22 0.39 2.53 * NaF 7.3 16.30 13.40 3.77 1.18 1.82 212.00 The above values are the means of triplicate experiments. The results show that Sn(NH4)2F6 was more inhibitory than the non-fluoride salts and NaF, with no apparent changes in specificities. The ID/50 values, recalculated as ppm F , again show (Table II) that Sn(NH4)2F6 is over-all more active than NaF. TABLE II INHIBITION OF BACTERIAL GROWTH BY FLUORINE SALTS ID/50 (ppm F) S.mutans S.salivarius S.sanguis L.casei 6715 OMZ-176 SS4 H-257 10556 10557 Salts NaF 138 309 255 72 22 35 352 * SnF 56 87 93 29 17 32 113 Sn(2MIF6 137 163 188 46 25 45 288 It should be noted that Sn(NH4)2F6, which is the stannic form of tin, was less active than the stannous form SnF2. Dental plaque is a film on the surface of teeth. This layer is the result of bacterial growth and is composed chiefly of microorganisms, proteinaceous materials and microbial by-products, such as glucans and organic acids. Plaque is not only primarily responsible for caries formation but it is also implicated in gingival diseases. Because the results described herein above have demonstrated that Sn(NH4)2F6 possesses antibacterial activity, it is obvious that Sn(NH4)2F6 will also reduce plaque formation. The following are examples of a toothpaste and a mouthwash composition of the invention EXAMPLE I The following is an example of a toothpaste composition according to the invention Silica xerogel 13.00 Silica aerogel 22.00 Sorbitol (70% solution) 41.50 Sn(NH4)2F6 0.24 Sodium lauryl sulphate 7.00 Colour 0.04 Flavour 3.00 Sodium Saccharin 0.12 Sodium Benzoate 0.06 Titanium Dioxide 0.35 Glycerine 7.53 Water Balance to 100% EXAMPLE 2 The following is an example of a mouthwash composition according to the present invention. Ethanol 15.00 Propylene Glycol 10.00 Glycerol 12.00 Flavour, Colour 0.90 Polyoxyethylene (20) monolaurate 2.10 Sodium lauryl sulphate 0.33 Sn(NH4 )2F6 0.024 Buffer 18.00 Hydrochloric acid (to pH 4.1) q s Water Balance to 100% Although the examples herein show the use of Sn(NH4)2F6 in dentifrice and mouthwash preparations, the compounds of the present invention may also be incorporated in other oral preparations or formulations such as for example chewing gums and lozenges.

Claims: CLAIMS: 1. An oral composition having anti-caries and anti-plaque activity comprising 0.01 to 38 by weight of stannic ammonium fluoride (Sn(NH4)2F6) , 2. An oral composition as claimed in Claim 1, wherein the amount of Sn(NH4)2F6 is sufficient to provide 1000 to 1500 ppm of available fluoride ion. 3. An oral composition as claimed in Claim 1 or Claim 2 in the form of a toothpaste or mouthwash. 4. Use of an oral composition as claimed in any of Claims 1 to 3 in controlling caries and piaque.

Inventors: VICCARO, JOHN PETER
Applicants: UNILEVER NV, UNILEVER PLC
Application year: 1983
Main classification: A61K7
IPC codes: A61Q11, A61K8