TRIAZOLE ANTI-FUNGAL AGENTS

Abstract: Triazole human, plant and seed antifungal agents of the formula:- where R is phenyl optionally substituted by 1 to 3 substi­ tuents each independently selected from F, Cl, Br, I, CF₃, C₁- C₄ alkyl and C₁-C₄ alkoxy, or R is a 5-chloropyrid-2-yl group; R¹ is -CN, -CSNH₂, or -CONR²R³ where either (a) R² is H or C₁-C₆ alkyl, and R³ is H, C₁-C₆ alkyl, benzyl, phenethyl, phenyl, -CH₂CF₃, adamantyl, pyridylmethyl, C₃-C₇ cycloalkyl, carbamoylmethyl, (C₂-C₄ alkenyl)methyl, 2-hydroxyethyl, 2-­ (dimethylamino)ethyl, 2-(methylthio)ethyl, 2-­ (methylsulphinyl)ethyl, 2-(methysulphonyl)ethyl or 2-­ phenoxyethyl; said benzyl, phenethyl, phenyl and phenoxy group being optionally ring-substituted by 1 or 2 substi­ tuents each independently selected from C₁-C₄ alkyl, C₁-C₄ alkoxy, F, Cl, Br, I and CF₃, (b) R² and R³ taken together with the nitrogen atom to which they are attached represent a group of the formula:- where R⁴ is H, C₁-C₄ alkyl, C₂-C₄ alkanoyl or (C₁-C₄ alkoxy) carbonyl; (c) R² is H or C₁-C₄ alkyl and R³ is C₂-C₄ alkanoyl or benzoyl, said benzoyl group being otionally substituted by 1 or 2 substituents each independently selected from F, Cl, Br, I, CF₃, C₁-C₄ alkyl and C₁-C₄ alkoxy; or (d) R² and R³ are both (C₁-C₄ alkoxy)carbonyl; R⁶ is H or CH₃; and R⁶ is H or CH₃; and their pharmaceutically and agriculturally accectable salts. The compounds are useful as human and agricultural fungicides.

Description: This invention relates to novel triazole derivatives which have antifungal activity and are useful in the treatment of fungal infections in animals, including humans, and as agricultural fungicides. According to the invention, there are provided compounds of the formula EMI1.1 where R is phenyl optionally substituted by I to 3 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; R1 is -CN, -CSNR2, or -CONR2R3 where either (a) R2 is H or C1-C6 alkyl, and R3 is H, C1-C6 alkyl, benzyl, phenethyl, phenyl, -CE2CF3, adamantyl, pyridylmethyl, C3-C7 cycloalkyl, carbamoylmethyl, (C2-C4 alkenyl)methyl, 2-hydroxyethyl, 2-(dimethylamino)ethyl, 2-(methylthio) ethyl, 2-(methylsulphinyl) ethyl, 2-(methyl- sulphonyl)ethyl or 2-phenoxyethyl; said benzyl, phenethyl, phenyl and phenoxy groups being optionally ring-substituted by I or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, C1, Br, I and CF3, (b) R2 and R3 taken together with the nitrogen atom to which they are attached represesnt a group of the formula: EMI2.1 EMI2.2 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; (c) R2 is H or C1-C4 alkyl and R3 is C 24 alkanoyl or benzoyl, said benzoyl group being optionally substituted by 1 or 2 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy; or (d) R2 and R3 are both (C1-C4 alkoxy)carbonyl; R5 is H or CH3; and R6 is E or CH3; and their pharmaceutically and agriculturally acceptable salts. The invention further provides a pharmaceutical or agricultural composition comprising a compound of the formula (I), or a pharmaceutically or agriculturally acceptable salt thereof, together with 2 pharmaceutically or agriculturally acceptable diluent or carrier. The invention further provides a compound of the formula (I), or a pharmaceutically acceptable salt thereof, for use in medicine, in particular for treating fungal infections in humans. The invention also provides a method of treating a plant or seed having a fungal infection, which comprises contacting said plant or seed or locus of said.plant with antifungally effective amount of said compound of the formula. (I) or agriculturally acceptable salt thereof. In one- aspect R is preferably phenyl substituted by 1 to 3 substituents, more preferably 1 or 2 substituents, each independently selected from F, Cl, Br, I and CF3. R is preferably 4-fluorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-iodophenyl, 4-trifluoromethylphenyl, 2-chlorophenyl, 2, 4-dichlorophenyl, 2,4-difluorophenyl, 2,5-difluorophenyl, 2-fluoro-4-chlorophenyl, 2-chloro-4-fluorophenyl, 2,4,6-trifluorophenyl and 4-bromo-2,5-difluorophenyl R is more preferably 2,4-dichlorophenyl, 4-chlorophenyl or 2,4-difluorophenyl. R is most preferably 2,4-dichlorophenyl or 2,4-difluorophenyl. RÚ is preferably -CN, -CSNH2, or CONRêR wherein either (a) Rê is H, CH3 or C2H5, and R is H, C1-C6 alkyl, p-chlorobenzyl, p-chlorophenethyl, p-methylphenethyl, -CH2CF3, l-adamantyl, 4-pyridylmethyl, cyclopropyl, carbamoylmethyl, -CH2 CH=CH2, 2-hydroxyethyl, 2-(dimethylamino)ethyl, 2-(methyl thiojethyl, 2-(methylsulphinylethyl), 2-(methylsulphonylethyl), p-chlorophenyl, or 2-(p-chlorophenoxy)ethyl; (b) Rê and R3 taken together with the nitrogen atom to which they are attached represent a group of the formula: EMI4.1 (c) R2 is H and R3 is acetyl, propionyl or p-chlorobenzoyl; or (d) Rê and R are both -COOCH3. R1 is more preferably -CONE2 or -CONH(C1-C4 alkyl), most preferably -CONH2, -CONH.CH3 or -CONH.C2H5. R5 and R6 are both preferably H. In the most preferred individual compounds, K is 2,4-dichlorophenyl or 2,4-difluorophenyl; RÚ is -CONH2, -CONHCH3 or -CONHC2H5; and R5 and R6 are H. In one aspect of the invention, there are provided compounds of the formula: EMI4.2 where R is phenyl optionally substituted by 1 to 3 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; and R1 is -CN or -CoNR2R3 where R2 is H, or C1-Cs alkyl, and R3 is H, C1-Cs alkyl, benzyl, -CH2CF3 or adamantyl, said benzyl group being optionally substituted on its phenyl ring portion by I or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, C1, Br, I and CF3, or Rê and R taken together with the nitrogen atom to which they are attached. represent a group of the formula EMI5.1 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; and their pharmaceutically acceptable salts. In another aspect of the invention, there are provided compounds of the formula: EMI5.2 where R is phenyl optionally substituted by 1 to 3 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; RÚ is -CN or -CONR2R where R2 is H or C1-C6 alkyl, and R is H, C1-C6 alkyl, benzyl, -CE2CF3 or adamantyl, said benzyl group being optionally sustituted on its phenyl ring portion by 1 or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, C1, Br, I and CF3, or R and R taken together with the nitrogen atom to which they are attached represent a group of the formula EMI6.1 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; and R5 is H or and their pharmaceutically acceptable salts. In a further aspect of the invention, there are provided compounds of the formula EMI6.2 where R is a phenyl group optionally substituted by I to 3 substituents each independently selected from F, Cl, Br, I, CF3, C -C alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; R5 and R6 are each independently H or CH3; and either (a) R2 is R or C1-C4 alkyl and R is C2-C4 alkanoyl or benzoyl, said benzoyl group being optionally substituted by I or 2 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy; or (b) R2 and R are both (C1-C4 alkoxy)carbonyl; and their pharmaceutically and agriculturally acceptable salts. Where the compounds of formula (I) contain at least one chiral centre, the invention includes both the resolved and unresolved forms. The invention also includes the novel intermediates of the formula: - EMI7.1 where R, R5 and R6 are as previously defined for formula (I). Other useful intermediates have the formula EMI7.2 where R is as defined for formula (I). The compounds of the formula (I) can be prepared as follows: (1) Compounds in which R1 is -CN and R5 and R6 are H can be prepared by the following general method: EMI8.1 The preferred source of cyanide ions are the alkali metal cyanide, particularly sodium and potassium cyanide. In a typical procedure, the compound (II) and sodium or potassium cyanide are heated together in a suitable organic solvent, e.g. dimethylformamide, at up to 1000 C, preferably 65-700C, for, say, up to 6 hours. It is preferred to add the cyanide dropwise to the solution of the oxirane over, say, 9 hour. After cooling the reaction mixture and pouring it into water, the desired product can be isolated and purified by conventional techniques. The starting materials of the formula (11) are in many cases known compounds (see e.g. European Patent Application Publication No. 44605 to I.C.I.) or can be prepared by routine methods as will be known to those skilled in the art, e.g.: EMI9.1 <tb> <SEP> (a) <SEP> Cl <SEP> CL <tb> <SEP> Br.CI2CO-) <SEP> -- <SEP> IC2C03, <SEP> N <tb> <SEP> NH <SEP> + <SEP> Br. <SEP> CH2 <SEP> X2CO3, <SEP> N <SEP> N2C <tb> <SEP> N <SEP> acetonitril <SEP> e <tb> <SEP> reflux, <SEP> 20 <SEP> hrs. <tb> <SEP> Trimethyl <SEP> <tb> <SEP> sulphoxonium <tb> <SEP> iodide <SEP> and <tb> <SEP> NaB <tb> <SEP> 0 <tb> <SEP> N <SEP> N-CR <SEP> -aE <tb> <SEP> LN' <SEP> C12 <tb> <SEP> Cl <tb> <SEP> or <SEP> (b) <tb> <SEP> Cl <SEP> 3uLi/-78 C- <SEP> Cl <tb> <SEP> bJ <SEP> " <SEP> n-Buti/-78OC <tb> <SEP> N <SEP> 3r <SEP> 4) <SEP> CBCCS(CB <SEP> )2/OOC <tb> <SEP> t <SEP> Br2/48% <SEP> ac.EIBr/lOOCC, <tb> <SEP> rr <SEP> hour. <tb> <SEP> 0 <tb> N-CR <SEP> Tr <SEP> imethyl <SEP> sul <SEP> p <SEP> ho <SEP> x <SEP> {{0 <SEP> 1,2,4-trfazole, <SEP> CCcHBr <tb> <SEP> 3 <SEP> O <SEP> C, <SEP> 2 <SEP> days <tb> <SEP> M <tb> <SEP> C1 <SEP> iodizes <SEP> CHCClj <SEP> aq. <tb> <SEP> NaOH <SEP> (20%),0ceimide, <tb> <SEP> 2 <SEP> hours, <SEP> 60 <SEP> C. <tb> (2) Compounds in which RÚ is -CN and R5 and R6 are each H or CH3 can be prepared by the following general route: EMI10.1 The preferred strong base in n-butyllithium. In a typical procedure, the nitrile is dissolved in a suitable solvent, e.g. dry tetrahydrofuran (TEF), and the resulting solution is then cooled to about 700 C. A solution of n-butyllithium in hexane is then slowly added dropwise. After stirring for about 1 hour at 700 C, the ketone (III) in a suitable solvent, e.g. dry TRF, is slowly added dropwise After stirring for about an hour at -700C glacial acetic acid in a little ThF is added and the reaction mixture is allowed to warm to 0 C. The product can then be isolated and purified conventionally. When one of R and R6 is H and the other is CH3, the product will exist in two diastereoisomeric forms and these can often be separated by chromatography (see Example 34). The starting. materials of the formula (III) are either known compounds or can be prepared by conventional methods- (see e.g. Preparations 3(i) and 4(B)). (3) Compounds in which RÚ is -CONH2 and R5 and R6 are H can be prepared as follows: EMI11.1 Preferably compound (IV) is used in its ethyl ester form. The acid is preferably supplied by using compound (IV) in an acid addition salt form, e.g. as the dihydrochloride. Alternatively the free base can be used and hydrogen chloride gas can be bubbled into the solution Typically the reactants are heated together for a short period, preferably under reflux in a suitable high-boiling organic solvent such as 1,2-dichlorobenzene (b.p. 178"), when the reaction is usually complete in about 15 minutes. The starting materials of the formula (IV) are obtainable conventionally, e.g. as follows: EMI11.2 (as dihydrochloride salt) (4) Compounds in which R1 is -C0NE(C1-C4 alkyl) or -C0N(C1-C4 alkyl)2 can be prepared by the alkylation of the corresponding starting materials in which RÚ is -CONH2. The alkylation is typically carried out by dissolving the starting material in a suitable organic solvent, e.g. dry ThF, followed by cooling to 0-50C. A strong base such as sodium hydride is then added. After stirring for a few minutes, an appropriate quantity of alkylating agent is added. The preferred alkylating agents are the alkali metal iodides and bromides. For mono-alkylation, only one equivalent of alkylating agent should be used, and, for dialkylation, at least 2 equivalents. The alkylated product can be isolated from the reaction mixture by conventional techniques. (5) Compounds in which RÚ is -CONRêR where Rê and R are as defined in (a) or (b) in formula (I) can also be prepared as follows. EMI12.1 Compound (V) is preferably used in the form of its "functional equivalent as an acylating agent", e.g. as an acid chloride or bromide, a mixed anhydride of the formula: EMI13.1 or as a C1-C4 alkyl , succinimido, phthalimido or benzotriazol-l-yl ester. All these "functional equivalents are preparable conventionally from the acid (V). The acid chlorides and bromides are for example preparable by reaction of said acid with thionyl chloride or bromide, the mixed anhydrides by reaction with a C2-C5 alkanoyl chloride, the C1-C4 alkyl esters by simple esterification, and the succinimido, phthalimido and benzotriazol-l-yl esters by reaction with N-hydroxysuccinimide, N-hydroxyphthalimide or l-hydroxybenzotriazole in the presence of a dehydrating agent such as dicyclohexylcarbodiimide. In fact, it is preferred to use the compounds (V) in the form of their succinimido esters of the formula EMI13.2 Thus in a typical procedure, dicyclohexylcarbodiimide dissolved in e.g. dry dioxan is added to a solution of the acid (V) and N-hydroxysuccinimide in e.g. dry dioxan. After stirring for a few hours at room temperature and filtering, the reaction is generally completed by stirring the solution of the compound (VII) with the amine K2R3NH at room temperature for a few hours in e.g. dry dioxan, after which the product can be isolated and purified by conventional means If compound (V) is reacted in its free acid form, the reaction should generally be carried out in the presence of a dehydrating agent such as dicyclohexylcarbodiimide. The C1-C4 alkyl esters can also be prepared as follows: EMI14.1 Generally some of the product (VIII) cyclises in situ under the reaction conditions to give the intermediate lactone (A). The ester (VIII) and lactone (A) can be separated chromatographically. The benzotriazol-l-yl esters have the formula: EMI15.1 These can be prepared as stated above. Thus in a typical procedure, dicyclohexylcarbodiimide, l-hydrowybenzotriazole and the acid (V) are stirred together at room temperature for a short period in e.g. dry dioxan. The reaction is generally completed by stirring the resulting intermediate (IX) with the amine RêR NH at room temperature until the reaction is complete, after which the product can be isolated and purified by conventional means. (6) Compounds of the formula (I) in which R1 is -CONE2 can be prepared by the -controlled hydrolysis of the corresponding compounds in which RÚ is -CN. Typically this hydrolysis is carried out by heating the starting nitrile at, say, 70-1000C, preferably 90-954C, with aqueous sulphuric acid, preferably 80%, until the formation of the amide is complete as monitored by t.l.c. Further hydrolysis to convert -CONH2 to -COOH can be carried out under similar conditions, if desired. The compounds in which K1 is -COOH are useful intermediates [see route (5) above. (7) The amides of the formula (I) in which R1 is -CONR R3 where R2 and R3 are as defined in (a) or (b) for formula (I) can also be prepared from the intermediates of the formula (A) as follows:- EMI16.1 where R, R5 and R6 are as defined for formula (I), and R2 and are as defined in (a) or (b) in formula (I). The reaction can be carried out by stirring the reactants together in a suitable solvent, e.g. ethanol, at room temperature until the reaction is complete. If necessary, the reaction mixture can be heated to accelerate the reaction. The product can be isolated and purified conventionally. (8) The compounds of the formula (I) in which Rê is H or C1-C4 alkyl and R3 is C2 -C4 alkanoyl or said optionally substituted benzoyl group, can be prepared by reacting a compound of the formula: EMI16.2 where Rê is as defined above in this method and R, R5 and R6 are as defined for formula (I), with either an acid halide or an acid anhydride of the formula: R .X or (R )2O where R3 is as defined above in this method and X is CI or Br. When an acid halide is used, the reaction is desirably carried out in the presence of a base such as pyridine or sodium hydride. The reaction is typically carried out in a suitable organic solvent, e.g. acetonitrile or tetrahydrofuran. It is not generally necessary to accelerate the reaction by heating, and the product can be isolated and purified conventionally. (9) The compounds of formula (I) in which K2 and R3 are both -C1-C4 alkoxy)carbonyl can be prepared by reacting a compound of the formula: EMI17.1 with at least two equivalents of an alkyl. haloformate of the formula: EMI17.2 where X is Ci or Br. The reaction can be carried out in a similar manner to route (8) above and again the presence of a base is desirable. (10) Compounds in which R3 is 2-(methylsulphinyl)ethyl and 2-(methylsulphonyl)ethyl can be prepared by the oxidation of the corresponding 2-(methylthio)ethyl compounds using the appropriate quantity of m-chloroperbenzoic acid in conventional manner. (11) Compounds in which RÚ is -CSNH2 can be prepared as follows: EMI18.1 The reaction is typically carried out by heating the reactants at up to about 100 C in the presence of water. The product can then be isolated conventionally. (12) Compounds in which K1 is -CONE2 and R5 and R6 are H can also be- prepared as follows: EMI18.2 The reaction is typically carried out by stirring bis(trimethylsilyl)acetamide at -700C in dry tetrahydrofuran (THF) whilst n-butyllithium is slowly added dropwise. The resulting solution is stirred at about -70 C for a short period, then the ketone (III) in e.g. dry TRF is slowly added, and the resulting mixture stirred at about -700C for a few hours. The reaction mixture is then allowed to warm to room temperature and aqueous acid is added. The product can then be isolated and purified conventionally. (13) Compounds in which R2 and R3 together with the nitrogen atom to which they are. attached represent aziridinyl can be prepared as follows: The reaction is generally carried out at room temperature in dry tetrahydrofuran. (14) The lactone intermediates of the formula (A) can be prepared by cyclisation, preferably using an ester according to the following scheme: EMI19.1 EMI19.2 where Q - C1-C4 alkyl, phthalimido, succinimido or 1-benzotriazolyl. These esters can be prepared as previously described. The cyclisation can be carried out in the presence of a suitable base by stirring at room temperature. Preferred bases are tertiary amine bases, e.g. triethylamine, and alkali metal hydrides, e.g. sodium hydride. The compounds of the invention contain a chiral centre or centres and the invention includes both the resolved and unresolved forms Pharmaceutically acceptable acid addition salts of the compounds of the formula (I) are those formed from strong acids which form non-toxic acid addition salts, such as hydrochloric, hydrobromic, sulphuric, oxalic and methanesulphonic acids. The salts may be obtained by conventional procedures, e.g. by mixing solutions containing equinolar amounts of the free base and desired acid, and the required salt is collected by filtration, if insoluble, or by evaporation of the solvent. Alsa included are the alkali metal salts, preparable conventionally. The compounds of the formula (I) and their pharmaceutically acceptable salts are antifungal agents, useful in combating fungal infections in animals, including humans. For example they are useful in treating topical fungal infections in man caused by, among other organisms, species of Candida, Trichophyton, Nicrosporum or Epidermophyton, or in mucosal infecions caused by Candida albicans (e.g. thrush and vaginal candidiasis). They. can also be used in the treatment of systemic fungal infections caused by, for example, Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus, Coccidioides, Paracoccidioides, Histoplasma or Blastomyces. The in vitro evaluation of the antifungal activity of the compounds can be performed by determining the minimum inhibitory concentration (m.i.c.) which is the concentration of the test compounds in a suitable medium at which growth of the particular micro-organism fails- to occur. In practice, a series of agar plates, each having, the test compound incorporated at a particular concentration is inoculated with a standard culture of, for example, Candida albicans and each plate is then incubated for 48 hours at 37"C. The plate are then examined for the presence or absence of growth of the fungus and the appropriate m.i.c. value is noted. Other micro-organisms used in such tests can include Cryptococcus neoformans, Aspergillus fumigates, Trichophyton spp; Microsporurs spp; Epidermophyton floccosum, Coccidioides immitis and Torulopsis glabrata. The in vivo evaluation of the compounds can be carried out at a series of dose levels by intraperitoneal or intravenous injection or by oral administration, to mice which are inoculated with a strain of Candida albicans. Activity is based on the survival of a treated group of mice after the death of an untreated group of mice following 48 hours observation. The dose level at which the compound provides 50% protection (PD50) 'against the lethal effect of the infection is noted. For human use, the antifungal compounds of the formula (I) can be administered alone, but will generally be administered in admixture with a pharmaceutical carrier selected with regard to the intended route of administration and standard pharmaceutical practice. For example, they can be administered orally in the form of tablets containing such excipients as starch or lactose, or in capsules or ovules either alone or in admixture with excipients, or in the form of elixirs or suspensions containing flavouring or colouring agents. They can be injected parenterally, for example, intravenously, intramuscularly or subcutaneously. For parenteral administration, they are best used in the form of a sterile aqueous solution which may contain other substances, for example, enough salts or glucose to make the solution isotonic with blood. For oral and parenteral administration to human patients, the do'ill dosage level of the antifungal compounds of the formula (I) will be from 0.1 to 5 mg/kg (in divided doses) when administered by either the oral or parenteral route. Thus tablets or capsules of the compounds will contain from 5 mg to 0.5 g of active compound for administration singly or two or more at a time as appropriate. The physician in any event will determine the actual dosage which will be most suitable for an individual patient and it will vary with the age, weight and response of the particular patient. The above dosages are exemplary of the average case; there can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention. Alternatively, the antifungal compounds of formula (I) can be administered in the form of a suppository or pessary, or they" may be applied topically in the form of a lotion, solution, cream, ointment or dusting powder. For example, they can be incorporated into a cream consisting of an aqueous emulsion of polyethylene glycols or liquid paraffin; or they can be incorporated, at a concentration between 1 and 10%, into an ointment consisting of a white wax or white soft paraffin base together with such stabilizers and preservatives as may be required. The compounds of the formula (I) and their salts also have activity against a variety of plant pathogenic fungi, including for example various rusts, mildews and moulds, and the compounds are thus useful for treating plants and seeds to eradicate or prevent such diseases. The in vitro evaluation of the activity of the compounds against plant fungi can be determined by measuring their'minimum inhibitory concentrations in the same way as previously described except that the plates are incubated at 30"C for 48 hours or longer before being examined for the presence or absence of growth. Micro-organisms used in such tests include Cochliobolus carbonum, Pyricularia oryzae, Glomerella cingulata, Penicillium digitatum, Botrytis cinerea and Rhizoctonia solani. For agricultural and horticultural purposes the compounds and their agriculturally acceptable salts are preferably used in the form of a composition formulated as appropriate to the particular use and purpose desired. Thus the compounds may be applied in the form of dusting powders, or granules, seed dressings, aqueous solutions, dispersions or emulsions, dips, sprays, aerosols or smokes. Compositions may also be applied in the form of dispersible powders, granules or grains, or concentrates for dilution prior to use. Such compositions may contain such conventional carriers, diluents or adiuvants as are known and acceptable in agriculture and horticulture and they are manufactured in accordance with conventional procedures. The compositions may also incorporate other active ingredients, for example, compounds having herbicidal or insecticidal activity or a further fungicide. The compounds and compositions can be applied in a number of ways, for example they can be applied directly¯to the plant foliage, stems, branches, seeds or roots or to the soil or other growing medium, and they may be used not only to eradicate disease, but also prophylactically to protect the plants or seeds from attack. The following Examples illustrate the invention. All temperatures are in OC:- EXAMPLE 1 Preparation of 1-cyano-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol l-yl) propan-2-ol EMI25.1 To 2-(2,4-Dichlorophenyl)-2-(1H-1,2,4-triazol-1-ylmethyl)oxirane (6.7 g) in dimethylformamide (198 ml) at 600 was added sodium cyanide (2.84 g) in water (49 ml) dropwise over 25 minutes. Heating at 60 was continued for 5 hours. The reaction mixture was then cooled, poured into water (900 ml), and extracted with ethyl acetate (3 x 150 ml). The combined organic extracts were washed with saturated aqueous brine, dried (Na2S04) and evaporated to dryness to give a pale yellow solid (6.1 g) which was triturated with ether. The residual solid was recrystallised from ethesJmethanol to give the title compound, 4.13 g (56X), m.p. 217-219". Analysis Z:- Found: C,48.3; H,3,4; N,18.4 Calculated for C12H10Cl2N4O: C,48.5; H,3.4; N,18.8. EXAMPLE 2 Preparation of 1-cyano-2-(2,4-dichlorophenyl)-3-(1H-1,2,4triazol-l-yl) propan-2-ol EMI26.1 Acetonitrile (2.25 g, 1.1 equivalent) was dissolved in dry tetrahydrofuran (100 ml) and the resulting solution was cooled to -70 under nitrogen in a C02/acetone bath. A solution of n-butyllithium in hexane (39 ml, 1.55 molar, 1.2 equivalent) was added dropwise over a 5 minute period. After stirring for about 45 minutes at -70 ¯, 2'4'-dichloro-2-(1H-1,2,4-triazol-1-yl) acetophenone (12.8 g) in dry tetrahydrofuran (100 ml) was added dropwise over a 15 minute period. Stirring was continued at -70 for about I hour and then glacial acetic acid (20 ml) in tetrahydrofuran (20 ml) was added dropwise. The cooling bath was then removed. The reaction mixture was allowed to warm to 0 C, quenched in water (400 ml), and. solid sodium carbonate was added to raise the pH to 8.0. After extraction with ethyl acetate (3 x 75 ml), the combined organic extracts were washed with saturated brine (3 x 50 ml), dried (Na2S04) and evaporated to a pale yellow solid. This solid was washed well with ether to give the title compound (6.61 g, 44.5X), identical to the product of Example 1 as confirmed by n.m.r. and i.r. spectroscopy. EXAMPLE 3 l-Cyano-2-(2,4-difluorophenyl)-3-(lE-1,2,4-trtazol-1-yl) propan-2-ol was prepared similarly to the previous Example using 2',4'-difluoro-2-(lE-1,2,4-triazol-1-yl)acetophenone as the starting ketone. It had an.m.p. of 154-54. Analysis t:- Found: C,54.0; H, 3,8; N,21.5 Calculated for C12H10F2N4O: C,54.6; H,3.8; N,21.2 EXAMPLE 4 Preparation of 1-carbamoyl-2-(2,4-dichlorophenyl)-3-(1H-1,2,4 triazol-l-yl) propan-2-ol EMI27.1 3-(2,4-Dichlorophenyl)-3-hydroxy-4-(1H-1,2,4-triazol-1-yl) butyrimidic acid, ethyl ester dihydrochloride (3.42 g) was suspended in 1,2-dichlorobenzene (35 ml) and the reaction mixture was heated to the reflux temperature of the solvent (178 ). After refluxing for 5 minutes, a solution was obtained. Refluxing was then continued for a further 10 minutes. The reaction mixture was then cooled, evaporated, and the resulting gum was triturated with hexane and heated with acetone. On cooling a cream coloured granular solid was formed which was filtered off to yield the title compound as a solvate (1.26 g). On standing overnight in a refrigerator some further solvated product precipitated (0.62 g) After drying at 80 C for 6 hours to remove the solvent the pure title compound was obtained, yield 1.5 g, m.p. 144-1454. Analysis Z (after said drying): Found: C,45.5; H,3.8; N,17.5 Calculated for C12H12C12N402: C, 45.7; H,3.8; ,l7.8. EXAMPLE 5 Preparation of 2-(2,4-dichlorophenyl)-1-(N-methylcarbamoyl)-3 (1H-1,2,4-triazol-1-yl)propan-2-ol EMI28.1 1-Carbamoyl-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazoll-yl)propan-2-ol (1.0 g) was dissolved in dry tetrahydrofuran (20 ml) and the reaction mixture was cooled to 0-5 . Sodium hydride (0.15 g, as a 50X dispersion in oil) was then added, followed by, after stirring for 10 minutes, methyl iodide (0.45 g). Further quantities of methyl iodide (90 mg) and sodium hydride (375 mg, as a 50Z dispersion in oil) were added. After stirring for a few minutes, yet further quantities of methyl iodide (90 mg) and sodium hydride (375 mg, as a 50% dispersion in oil) were added. The mixture was then quenched in water and extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were dried (MgSO4) and evaporated to give the crude product as a gum. A solution of this gum in methylene chloride (20 ml) was chromatographed on a "Kieselgel 60H" (Trade Mark) column (10 g), eluting with methylene chloride (100 ml), then with methylene chloride containing 2 vol.% isopropanol and 0.2 vol.% NH40E (300 m1), and finally with methylene chloride containing 5 vol.Z isopropanol and 0.5 vol.% NE40H (500 ml)., Appropriate fractions were collected to yield the title compound, which was recrystallised from cyclohexane (yield 41 mg, m.p. 151-4 ). Analysis Z: Found: C,47.3; R,4.35; N,17.2 Calculated for C13H14Cl2N4O2: C,47.4; H,4.3; N,17.0. EXAMPLE 6 Preparation of 2-(2,4-dichlorophenyl)-1-(morpholinocarbonyl)-3 (1H-1,2,4-triazol-1-yl)propan-2-ol monohydrate EMI30.1 N,N'-Dicyclohexylcarbodiimide ("DCCD") (110 mg, 0.5 mmole) dissolved in dry dioxan (5 ml) was added to a solution of 1-carboxy-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol-1-yl)propan2-ol (150 mg, 0.5 mmole) and N-hydroxysuccinimide ("NHS") (60 mg, 0.5 mmole) in dry dioxan (10 ml), and the mixture was stirred at room temperature for 2 hours. The' precipitate was filtered off, washed with dry dioxan (10 ml) and the combined filtrate and washings were then added to a solution of morpholine (300 mg, 3.4 mmole) in dry dioxan (2 ml). The resultant solution was left at room temperature for 18 hours, diluted with ethyl acetate (100 ml), washed three times with saturated brine solution and dried over magnesium sulphate. Evaporation of the filtrate gave an oil (300 mg) which was then chromatographed on "Kieselgel 60H'1 (Merck, Trade Mark) silica (10 g), eluting with CE2C12 containing 2 vol.% isopropyl alcohol and 0.2 vol.Z aq. ammonium hydroxide (S.G. 0.880). The title compound was obtained after evaporation of appropriate fractions as a colourless solid, 110 mg (57%), m.p. 92-93e. Analysis Z: Found: C,47.8; H,4.7; N,13.9 Required for C16H18N4Cl2O3.H2O: C,47.8; H,5.0; N,13.9. EXAMPLES 7-32 The following compounds were prepared similarly to the previous Example, starting from the same acid, DCCD/NHS and the appropriate amine:- EMI31.1 EMI31.2 <tb> Example <SEP> R1 <SEP> m.p. <SEP> Analysis <SEP> Z <tb> <SEP> No. <SEP> (eC) <SEP> Theoretical <SEP> in <SEP> brackets <tb> <SEP> C <SEP> H <SEP> N <tb> <SEP> 7 <SEP> -CONHCH3 <SEP> 151-3" <SEP> (Identical <SEP> to <SEP> the <tb> <SEP> product <SEP> of <SEP> Example <SEP> 5) <tb> <SEP> 8 <SEP> -CONHCH(CH3)2 <SEP> 105-107 <SEP> 50.7 <SEP> 5.2 <SEP> 15.3 <tb> <SEP> (50.6 <SEP> 5.1 <SEP> 15.7) <tb> <SEP> I25-126.5 <SEP> 48.95 <SEP> 4.65 <SEP> 16.3 <tb> <SEP> (49.1 <SEP> 4.7 <SEP> 16.4) <tb> <SEP> 10 <SEP> -CONECH2 <SEP> 5 <SEP> 1 <SEP> glass <SEP> 52.15 <SEP> 3.95 <SEP> 12.5 <tb> <SEP> 64-65" <SEP> (52.05 <SEP> 3.9 <SEP> 12.8) <tb> EMI32.1 <tb> <SEP> Example <SEP> R1 <SEP> m.p. <SEP> Analysis <SEP> X <tb> <SEP> No. <SEP> (OC) <SEP> Theoretical <SEP> in <SEP> brackets <tb> <SEP> C <SEP> H <SEP> N <tb> <SEP> m\ <SEP> glass, <SEP> (As <SEP> trthydrate) <tb> <SEP> II <SEP> -cON <SEP> NCOCH3 <SEP> 63-50 <SEP> 45.3 <SEP> 4.4 <SEP> 14.4 <tb> <SEP> (45.1 <SEP> 5.6 <SEP> 14.6) <tb> <SEP> /m <SEP> glass, <SEP> (As <SEP> hemihydrate) <tb> <SEP> 12 <SEP> -CON <SEP> NCO2C <SEP> H5 <SEP> 58-600 <SEP> 49.2 <SEP> 5.1 <SEP> 15.0 <tb> <SEP> 2 <tb> <SEP> (49.1 <SEP> 5.Z <SEP> 15.1) <tb> <SEP> 13 <SEP> -CON <SEP> g <SEP> glass, <SEP> 52.4 <SEP> 5.05 <SEP> 15.0 <tb> <SEP> 1 <SEP> 40-41" <SEP> (52.2 <SEP> 4.9 <SEP> 15.2) <tb> <SEP> 14 <SEP> -CON(C2H5)2 <SEP> glass, <SEP> 51.9 <SEP> 5.5 <SEP> 15.0. <tb> <SEP> 60-620 <SEP> (51.8 <SEP> 5.4 <SEP> 15.1) <tb> 15 <SEP> -CONRC2H5 <SEP> i <SEP> l29130o <SEP> 49.0 <SEP> 4.8 <SEP> 15.8 <tb> <SEP> (49.0 <SEP> 4.7 <SEP> 16.3) <tb> <SEP> 16 <SEP> -CONH(I-adainantyl) <SEP> 91-92" <SEP> 58.7 <SEP> 6.1 <SEP> 12.0 <tb> <SEP> (58.8 <SEP> 5.8 <SEP> 12.5) <tb> <SEP> glass, <SEP> 52.3 <SEP> 4.2 <SEP> 16.5 <tb> <SEP> 48-50' <tb> <SEP> (52.1 <SEP> 4.3 <SEP> 16.8) <tb> <SEP> glass, <SEP> 42.6 <SEP> 42.6 <SEP> 3.5 <SEP> 13.6 <tb> <SEP> 2 <SEP> 3 <tb> <SEP> 60-62" <tb> t <SEP> } <SEP> l <SEP> (54.1 <SEP> 6.1 <SEP> 14.0) <tb> EMI33.1 <tb> <SEP> I <tb> <SEP> Example <SEP> R1 <SEP> m.p. <SEP> Analysis <SEP> Z <tb> <SEP> No. <SEP> (OC) <SEP> Theoretical <SEP> in <SEP> brackets <tb> C <SEP> H <SEP> B <SEP> N <tb> <SEP> 20 <SEP> -0088- <SEP> 1 <SEP> 122-123 <SEP> 50.6 <SEP> 4.6 <SEP> 15.6 <tb> <SEP> (50.7 <SEP> 4.5 <SEP> 15.8) <tb> 21 <SEP> -CoND <SEP> ffi <SEP> CE2 <SEP> b <SEP> l <SEP> 142-143 <SEP> / <SEP> 52.9 <SEP> 4.2 <SEP> 12.2 <tb> <SEP> (52.9 <SEP> 4.2 <SEP> 12.3) <tb> <SEP> (As <SEP> hydrochloride) <tb> <SEP> 22 <SEP> -CONE(C82)2 <SEP> 169-170 <SEP> 45.7 <SEP> 4.8 <SEP> 14.0 <tb> <SEP> (45.8 <SEP> 4.9 <SEP> 14.2) <tb> <SEP> (As <SEP> hydrochloride <tb> <SEP> hemihydrate) <tb> <SEP> 23 <SEP> -CONECa2CO <SEP> F <SEP> 188-1910 <SEP> 40.6 <SEP> 4.05 <SEP> 16.9 <tb> - <SEP> (40.2 <SEP> 4.1 <SEP> 16.8) <tb> <SEP> (As <SEP> oxalate <SEP> F <SEP> hydrate) <tb> <SEP> 24 <SEP> GONHCII-CBC <SEP> glass <SEP> 45.4 <SEP> 4.3 <SEP> 12.0 <tb> <SEP> (45.4 <SEP> 4.1 <SEP> 12.4) <tb> <SEP> 25 <SEP> -CONHCH2C(CR3)3 <SEP> 135-137 <SEP> 53.2 <SEP> 5.9 <SEP> 14.7 <tb> <SEP> (53.0 <SEP> 5.8 <SEP> 14.5) <tb> <SEP> l <SEP> <tb> <SEP> (As <SEP> 1/4 <SEP> hydrate) <tb> !26C0NHCH2CH2oH <SEP> 26 <SEP> -CONB8, <SEP> 08,08 <SEP> 143-145 <SEP> 046.1 <SEP> 4.4 <SEP> 15.3 <tb> (46.2 <SEP> 4.6 <SEP> 15.4) <tb> <SEP> 27 <SEP> -CONE(CE2)2 <SEP> 144-145" <SEP> 58.2 <SEP> 5.1 <SEP> 12.8 <tb> 22 <tb> <SEP> (58.2 <SEP> 5.1 <SEP> 12.9) <tb> EMI34.1 <tb> Example <SEP> | <SEP> R <SEP> m.p. <SEP> Analysis <SEP> Z <tb> <SEP> No. <SEP> al <SEP> No, <SEP> ) <SEP> Theoretical <SEP> in <SEP> brackets <tb> <SEP> C <SEP> H <SEP> N <tb> <SEP> (As <SEP> dihydrochloride <tb> <SEP> dihydrate) <tb> <SEP> 28 <SEP> -C0NH()2NCCH3)2 <SEP> 107-fl00 <SEP> 38.8 <SEP> 5.2 <SEP> 13.6 <tb> <SEP> (38.8 <SEP> 5.1 <SEP> 14.2) <tb> <SEP> (Contains <SEP> 1 <SEP> mole <SEP> of <tb> <SEP> 9 <SEP> f\ <SEP> cycloherane <SEP> -) <tb> <SEP> 29 <SEP> -C0NEl. <SEP> \\ <SEP> 1 <SEP> 102-105 <SEP> " <SEP> 56.6 <SEP> cycloh5ex4ane' <SEP> 5.4 <SEP> 10.9 <tb> <SEP> (56.5 <SEP> 5.3 <SEP> 11¯0) <tb> <SEP> 30 <SEP> -CCNHCNzCRzSCH3 <SEP> 154-1560 <SEP> 42.6 <SEP> 4.5 <SEP> 13.3 <tb> <SEP> (42.3 <SEP> 4.5 <SEP> 13.2) <tb> <SEP> 31 <SEP> ONHCEzCH2(ffC1 <SEP> 1379O <SEP> 51.0 <SEP> 4.0 <SEP> ll.9 <tb> <SEP> (51.1 <SEP> 4.1 <SEP> it. <SEP> 9) <tb> <SEP> 32 <SEP> p <SEP> 0N(CH3)(CH(cH]2) <SEP> 131-2 <SEP> 51.7 <SEP> 5.3 <SEP> 15.2 <tb> <SEP> (51.8 <SEP> 5.4 <SEP> 15.1) <tb> EXAMPLE 33 The following compound was prepared similarly to Example 6, starting from l-carboGy-2-(2,4-difluorophenyl)-3-(1R-1,2,4- triazol-1-yl)propan-2-ol, "DCCD", "NHS" and methylamine: EMI35.1 It had an m.p. of 129-131 . Analysis X: Found: C,52.8; H,4.9; N,19.3 Calculated for C13H14F2N4O2: C,52.7; H,4.8; N,18.9. EXAMPLE 34 Preparation of 3-cyano-2-(2,4-dichlorophenyl) -1-(1H 1,2,4-triazol-1-yl)butan-2-ol (2 diastereoisomeric-forms) EMI35.2 Propionitrile (1.21 g) in dry tetrahydrofuran (50 ml) was cooled to -720. A solution of n-butyllithium as a solution in avhexane (14.2 ml, 1.55 molar) was then slowly added whilst maintaining the temperature of the reaction mixture at -450 or below. After stirring for about 30 minutes, 2-(lH-I,2,4- triazol-l-yl)-2',4'-dichloroacetophenone (2.56 g) in dry tetrahydrofuran (TRF) (50 ml) was added slowly with stirring over a 20 minute period, the temperature of the mixture being maintained at -700. Stirring was continued at this temperature for 1 hour and then at 100 for 1/2 hour when glacial acetic acid (10 ml) in dry THF (15 ml) was added. The reaction mixture was then allowed to warm to room temperature (20 ), was basified to pE8 with solid sodium bicarbonate, and was extracted with ethyl acetate (3 x 75 ml). The combined organic extracts were washed three times with water, dried (MgSO4), evaporated and ether (30 m1) was added to the residue, yielding a white crystalline solid and a yellow solution. The solid was filtered off, dissolved in a small volume of methylene chloride, and loaded onto an 18 g flash chromatography column of Merck's "Kieselgel 60" (Trade Mark) 230-400 mesh silica in ether CII x 2 cm. diameter). Elution was carried out using 5X (by volume) acetone in ether at 1 p.s.i. "Diastereoisomer 1" of the title compound was eluted first, 0.79 g, m.p. 178-180 . Analysis %: Found: C,50.0; H,3.8; N,17.9; Calculated for C13H12Cl2N4O: C,50.2; H,3.9; N,18.0. "Diastereoisomer 2" of the title compound was eluted next, 0.244 g, m.p. 202-205 . Analysis X:- Found: C,50.4; H,3.9; N,17.6; Calculated for C13H12Cl2N4O: C,50.2; H,3.9; N,18.0. EXAMPLES 35 AND 36 The following compounds were prepared similarly to the previous Example, starting from the appropriate acetophenone, n-BuLi/C2H5CN and glacial acetic acid: EMI37.1 EMI37.2 <tb> <SEP> stample <SEP> m.p. <SEP> Analysis <SEP> X <tb> <SEP> No, <SEP> (OC) <SEP> Theoretical <SEP> in <SEP> brackets <tb> <SEP> 'C <SEP> H <SEP> N <tb> <SEP> C <SEP> E <SEP> N <SEP> &verbar; ; <tb> <SEP> 35 <SEP> W <SEP> l59l620 <SEP> 56.4 <SEP> 4.8 <SEP> 20.0 <tb> (mixture <SEP> of <SEP> (56.4 <SEP> 4.7 <SEP> 20.2) <tb> <SEP> liastereomers <tb> <SEP> ot <SEP> separated) <tb> <SEP> 4 <SEP> t <SEP> 185-l87 <SEP> 56.2 <SEP> 4.3 <SEP> 20.0 <tb> <SEP> (believed <SEP> to <SEP> be <SEP> a <SEP> (56.1 <SEP> 4 <SEP> 4.3 <SEP> 20.1) <tb> <SEP> mixture <SEP> of <tb> <SEP> diastereomers, <SEP> not <tb> <SEP> separated) <tb> EXAMPLE 37 Preparation of 3-carbamoyl-2-(2,4-dichlorophenyl)-1- (1H-1,2,4triazol-1-yl)butan-2-ol hemihydrate and 3-carboxy-2- (2, 4-dichloro- phenyl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol EMI38.1 3-Cyano-2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol1-yl)butan-2-ol (700 mg, diastereoisomer I from the previous Example) was heated for 5 1/2 hours at 90-95 in 40% (by volume) aqueous sulphuric acid The solution was then stirred at room temperature (200) fpr 19 hours, after which time saturated aqueous sodium bicarbonate solution was added to raise the pR to 8.0. The solution was then extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were washed with water, dried (MgS04) -and evaporated to yield the 3-carbamoyl title compound, 105 mg, m.p. 215-217 after trituration with ether. Analysis X:- (3-carbamoyl compound) Found: C,46.8; H,4.5; N,15.5; Calculated for C13H14Cl2N4O2.1/2H2O: C,46.2; H,4.5; N,15.6. The aqueous phases resulting from the ethyl acetate extractions were combined, acidified to pH 2.0 with dilute hydrochloric acid, and extracted with ethyl acetate (3 x 50 ml). The combined organic extracts were washed with water, dried (MgSO4) and evaporated to yield the title acid. After trituration with ether, the pure acid, 485 mg, m.p. 236-238 , was obtained. Analysis X: Found: C,47.0; H,3.9; N,12.4; Calculated for C13H13Cl2N3O3: C,47.3; H,4.0; N,12.7. EXAMPLE 38 3-Carbamoyl-2-(2,4-difluorophenyl)-1-(1H-1,2,4-triazol-1-yl) butan-2-ol 1/4 hydrate, m.p. 170-172 , was prepared similarly to the previous Example by the hydrolysis of the corresponding nitrile prepared in Example 36 but using 80% aqueous sulphuric acid. Analysis X:- Found: C,52.0; H,4.8; N,18.5; Calculated for C13H14F2N4O2.1/4 H20: C,51.9; H,4.8; N,18.6. EXAMPLE 39 Preparation of 2(2, 4-difluorophenyl)-3- (N-methyl- carbamoyl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol EMI40.1 The mixture of diastereomeric nitriles from Example 35 (3.9 g) was heated in sulphuric acid (80Z, 100 ml) for 4 hours at 600. The reaction mixture was then cooled, diluted with water (200 ml), and calcium carbonate (50 g) was added in small portions with external cooling (ice bath). The mixture was then filtered, and the material which had been filtered off was washed well with water (200 ml) and methanol (200 ml). The washings were added to the filtrate, evaporated to dryness, and the residue extracted with ethyl acetate (3 x 100 ml). The extracts were combined, dried (MgS04), and evaporated to a white solid, 2.73 g. This material was absorbed onto silica ("Kieselgel 60", 7 g) by dissolution in the minimum quantity of a chloroform:methanol mixture (5:1, v/v), addition of the silica, and evaporation of the solvents. This silica was added as a suspension in ether to a silica column ("Kieselgel 60", 25 g), and eluted with ether containing .an increasing proportion of ethanol (2 0x). A proportion of the least polar amide diastereoisomer was eluted first in a pure state; and was recrystallised from ethyl acetate to give colourless crystals of one isomer of the title compound, m.p. 223-225 , 105 mg. Analysis Z: Found: C,52.8; H,5.3; N,18.7 C13H15ClN4O2 requires: C,53.0; H,5.1; N,19.0. The remainder of the product was eluted as a mixture containing both the diastereoisomer characterised above and its more polar diastereomer (1:4 by n.m.r.). Kecrystallisation from ethyl acetate gave colourless crystals, m.p. 186-189 , 404 mg. Analysis Z: Found: C,53.0; H,5.1; N,19.4; C13H15ClN4O2 requires: C,53.0; H,5.1; N,19.0. EXAMPLE 40 Preparation of 2-(2,4-dichlorophenyl)-3-CN-cethvlcarbamoyl)-1-(1H-1,2,4-triazol-1-yl)butan-2-ol EMI41.1 3-Carboxy-2-(2, 4-dichlorophenyl)-l-(lH-l, 2,4- triazol-lyl)butan-2-ol (330 mg) was added to dry dioxan (10 ml) followed by l-hydroxybenzotriazole hydrate ("ROBT") (203 mg) and dicyclohexylcarbodiimide ("DCCD") (618 mg). After stirring for 1 hour at room temperature (200), methylamine (278 mg of 33% [by volume] solution in ethanol) was added and stirring was continued overnight (20 hours). After this time, the resulting precipitate of dicyclohexylurea was filtered off. The filtrate was added to water (50 ml) and solid sodium bicarbonate was added to pH8. The mixture was then extracted with ethyl acetate (3 x 50 ml) and the combined organic extracts were washed with water, dried (MgSO4) and evaporated. The residue was dissolved in a small volume of methylene chloride and chromatographed on a.Merck "Kieselgel 60" (Trade mark) silica flash column in ether. Elution -with ether (100 ml) followed by 15X (by volume) ethanol in ether (300 ml) yielded, by collection of appropriate fractions, the title compound, 29 mg, m.p. 242-2440. Since the filtered-off dicyclohexylurea contained a further quantity of the title compound, this was dissolved in a small amount of methanol and absorbed onto Merck's '1Kieselgel 60"" (Trade mark) silica (3 g), and the resulting slurry was then loaded onto a 10 g flash column of this material in ether. Elution with 10% (by volume) ethanol in ether, and collection of appropriate fractions followed by recrystallisation from isopropanol, yielded a further quantity of the title compound (81 mug). Analysis X:- Found: C,48.9; H,4.8; N,16.2; Calculated for C14H16Cl2N4O2: C,49.0; H,4.7; N,16.3; EXAMPLE 41 (A) Preparation of 2-(2,4-dichlorophenyl)-3-ethoxycarbonyl-3methyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol and 4-(2,4dichlorophenyl)-3,3-dimethyl-4-(1H-1,2,4-triazol-1-ylmethyl) ss-propiolactone EMI43.1 2-(1H-1,2,4-Triazol-1-yl)-2',4'-dichloroacetophenone (2.56 g) in dry tetrahydrofuran (20 ml) and ethyl&alpha;-bromoisobutyrate (1. 475 ml) in dry ether (10 ml) were added simultaneously to granulated zinc (1.S g) in toluene (10 ml) over 20 minutes. The reaction mixture was then heated at 800 for 18 hours. The cooled reaction mixture was poured onto ice-cold sulphuric acid (0.2N, 125 ml) and extracted with ether (200 ml). The ether extract was washed with brine, dried (MgS04), and concentrated in vacuo. The residue was flash, chromatographed on silica (120 g) and eluted with 80Z ethyl acetate/20Z hexane The initial fractions yielded the title ester, which was crystallised from ethyl acetate/hexane, yield of the pure product 61 mg, m.p. 95-96 . Analysis Z: Found: C,51.7; H,5.2; N,11.1; Calculated for C16H19Cl2N3O3: C,51.6; H,5.1; N,11.3. The later fractions on evaporation gave the title lactone, which was recrystallised from ethyl acetate/hexane, yield of the pure product 240 mg, m.p. 177-178 . Analysis Z:- Found: C,51.8; H,3.9; N,12.8; Calculated for C14H13Cl2N3O2: C,51.5; H,4.0; N,12.9. (B) Preparation of 3-carbamoyl-2-(2,4-dichlorophenyl)- 3-methyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol EMI45.1 To a solution of. 2-(2,4-dichlorophenyl)-3-ethoxycarbonyl3-methyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol (75 mg) in ethanol (5 ml), aqueous ammonia aqueous ammonia (S.G. 0.88, 12 ml.) was added. and the solution was left at room temperature (200) for 8 days. The solvent was then evaporated in vacuo, the residue was partitioned between methylene chloride and water, and the organic extracts were washed with brine and dried (MgSQ4). Removal of solvent followed by flash chromatography on silica (30 g) and elution with a mixture of methylene chloride/methanol/ammonfla (93:7:1) gave the title compound, m.p. 162-30 (34.5 mg). Analysis t:- Found: C,48.8; R,4.7; N,15.8; Calculated for C14H16Cl2N4O2: C,49.O; H,4.7; N,l6.3. (C) 3-Carbamoyl-2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1yl)propan-2-ol was prepared similarly to parts (A) and (B) above from appropriate starting materials, and was confirmed spectroscopically to be. identical to the product of Example 4. (C) Preparation of 3-carbamoyl-2-(2,4-dichlorophenyl)-3- methyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol [alternative method to (B) above] EMI46.1 A solution of 4-(2,4-dichlorophenyl)-3,3-dimethyl-4-(1H- 1,2,4-triazol-1-yl-methyl)-ss-propiolactone (70 mg) in ethanol (4 m1) was treated with 0.88 ammonia (6 ml) and left to stand at room temperature for 5 days. The reaction mixture was then evaporated in vacuo and extracted and chromatographed by the method described in part (B) above to yield the title compound, m.p. 162-30, (41 mg). Analysis t:- Found: C,48.6; H,4.7; N,l5.9; Calculated for C14H16Cl2N4O2: C,49.0; H,4.7; N,16.3. EXAMPLE 42 Preparation of 2-(2,4-dichlorophenyl)-3-methyl-3-(N methylcarbamoyl)-l-(lE-1,2,4-triazol-1-yl)butan-2-ol EMI47.1 A solution of 4-(2,4-dichlorophenyl)-3,3-dimethyl-4-(1H- 1,2,4-triazol-1-ylmethyl)-ss-propiolactone (200 mg) in ethanol (5 ml) was treated.with a solution of 354 (by volume) methylamine in ethanol. (5 ml), and the resulting solution was left to stand overnight at room. temperature (200). After evaporating residual methylamine and ethanol, the residue was triturated with hexane and the resulting solid was crystallised from ethyl acetate/hexane to yield the title compound, m.p. 145-6 , (120 mg). Analysis %: Found: C,50.2; H,5.0; N,15.9; Calculated for C15H18Cl2N4O2: C,50.4; H,5.0; N,15.7. EXAMPLE 43 (A.) Preparation of 4-(2,4-dichlorophenyl)-4-(1H-1,2,4-triazol- l-ylmethyl)-g-propiolactone EMI48.1 3-Carboxy-2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1yl)butan-2-ol (948 mg) was dissolved in dry dioxan (20 ml) and l-hydroxybenzotriazole hydrate (0.61 g) followed by dicyclohexylcarbodiimide (1.85 g), were then added. The resulting mixture was stirred at room temperature (200) for 2 hours when triethylamine (455 mg) was added and stirring was continued overnight (19 hours). The mixture was then added to water (100 ml) and extracted with ethyl acetate (3 x 50 ml). The precipitate of dicyclohexylurea was filtered off after the first extraction. The combined organic extracts were washed with water, dried (MgS04) and evaporated. The resulting residue was dissolved in a little methylene chloride and loaded onto a flash column of Merck's "Kieselgel 60" (Trade mark) silica (12 g, 230-400 mesh) in ether. Elution with ether (100 ml) and then with 5% (by volume) acetone in ether (300 ml) gave, after collection of appropriate fractions, the title compound, 600 mg, m.p. 178-180". Analysis %: Found: C,48.1; H,3.0; N,14.0; Calculated for C12H9Cl2N3O2: C,48.4; H,3.0; N,14.1. (B.) Preparation of 3-(N-methylcarbamoyl)-2-(2,4-dichlorophenyl) 1H-(1,2,4-triazol-1-yl)propan-2-ol EMI49.1 This reaction was carried out similarly to Example 42 using the starting materials specified in the reaction scheme to give the title compound, confirmed spectroscopically to be the desired product and to be identical to the product of Example 5. EXAMPLE 44 Preparation of 3-carbamoyl-2-(2,4-dichlorophenyl)-1H (1,2 ,4-triazol-1-yl)propan -2-ol EMI49.2 This reaction was carried out similarly to Example 41(C) using the starting materials specified in the reaction sequence to give the title compound, confirmed spectroscopically to be the desired product and to be identical to the product of Example 4. EXAMPLE 45 Preparation of 3-(N-acetylcarbamoyl)-2-(2,4-dichlorophenyl)-1 (1H-1,2,4-triazol-1-yl)propan-2-ol hemihydrate EMI50.1 A solution of acetyl chloride (0.12 g, 1.5 mMole) in dry acetonltrile (2 ml.) was added dropwise to a stirred solution of 3-carbamoyl-2-(2,4-dichlorophenyl) IH-1,2,4-triazol-1-yl)- propan-2-ol (0.314 g., I mMole) and pyridine (0.12 g., 1.5 mMole) in dry acetonitrile (2 ml.) at -20 . The solution was allowed to warm- to room temperature and was stirred at room temperature for a further 18 hours. The solvent was then removed under reduced pressure and the residue was partitioned between water (10 ml.) and chloroform (40 ml.). The organic phase was separated, dried over MgSO4 and evaporated. The residue was chromatographed on silica eluting with 10:1 (v:v) chloroform/methanol and the fractions containing the product were combined, evaporated, and recrystallised from diisopropyl ether to furnish the title compound, (67 mg., 19%), m.p. 148-500. Analysis X: Found: C,45.8; H,3.9; N,15.0 Calculated for C14H14N4O3Cl2.1/2H2O: C,45.9; H,4.1; N,15.3. EXAMPLES 46 AND 47 The following compounds were prepared similarly to Example 45, starting from the same amide, pyridine, and the appropriate acid chloride of the formula R3.C1: EMI51.1 EMI52.1 <tb> <SEP> I <tb> <SEP> Example <SEP> R3 <SEP> m.p. <SEP> (OC) <SEP> Analysis <SEP> Z <SEP> (Theoretical <tb> <SEP> No. <SEP> in <SEP> brackets) <tb> <SEP> C <SEP> E <SEP> N <tb> <SEP> 46 <SEP> CE3CE2 <SEP> - <SEP> 101-3 <SEP> , <SEP> 48.2 <SEP> 4.3 <SEP> 14.7 <tb> <SEP> 0 <SEP> (48.5 <SEP> 4.3 <SEP> 15.1) <tb> 1 <SEP> I <tb> <SEP> 47 <SEP> C <SEP> 183-49 <SEP> 50.3 <SEP> 3.4 <SEP> 12.1 <tb> o <SEP> a <SEP> (50.3 <SEP> 3.3 <SEP> 12.3) <tb> EXAMPLE 48 Preparation of 2-(2,4-dichlorophenyl)-3-(N,N-dimethoxycarbonylcarbamoyl)-1-(1H-1,2,4-triazol-1-yl)propan-2-ol EMI52.2 3-Carbamoyl-2-(2,4-Dichlorophenyl)-1-(1H-1,2,4-triazol-1 yl)propan-2-ol (0.94 g., 3 mMole) was added to a suspension of oil-free sodiuiabydride (0.14 g., 5.8 mMole) in dry tetrahydrofuran (10 ml.). The suspension was stirred for 1 hour and then a solution of methyl chloroformate (0.6 g., 6.3 mMole) in dry tetrahydrofuran (10 ml.) was added dropwise over 15 minutes. The mixture was stirred at room temperature for 3 hours and then the solvent was evaporated in vacuo. The residue was partitioned between saturated aqueous sodium bicarbonate solution (10 ml.) and ethyl acetate (40 ml.), and the organic phase was separated, washed with brine (10 ml.), dried (MgSO4) and evaporated. The residue was chromatographed on silica eluting with ethyl acetate/methanol 95:5 (v;v), and the fractions containing the product were combined and evaporated and recrystallised from ethyl acetate-hexane to give the title compound, (62 mg., 5%), m.p. 155-60. Analysis Z:- Found: C,44.5; H,3.7; N,13.2 Calculated for C16H16N4O6Cl2: C,44.5; H,3.7; N,l3.O. EXAMPLE 49 Preparation of 1-carbamoyl-2-(2,4-dichlorophenyl)-3-(1H-1,2,4 triazol-l-yl)propan-2-ol EMI53.1 Bis(trimethylsilyl)acetamide (1.99 g) was stirred at -700 in dry tetrahydrofuran (15 ml) whilst n-butyllithium in hexane (6.3 ml, 1.55 M) was added dropwise over ten minutes. The resulting solution was stirred at -70 for 30 minutes, then a solution of 2-(1H-1,2,4- triazol-1-yl)2',4'-dichloroacetophenone (1.0 g) in dry tetrahydrofuran (10 ml) was added dropwise over 10 minutes, and the mixture was stirred for 1 1/2 hours at -70F. The reaction mixture was then allowed to warm to room temperature, and water (5 ml) and hydrochloric acid (7 ml, 2N) were added. The mixture was then adjusted to pH8 by the addition of solid sodium bicarbonate, and then extracted with ethyl acetate (3 x 10 ml). The combined extracts were washed with saturated sodium chloride solution (3 x 10 ml), dried (MgSO4), and evaporated to a gum, 1.1 g. This gum was chromatographed on silica ("Kieselgel 60", Merck), eluting with ether containing 5Z by volume ethanol. After the elutiow of unreacted ketone, the product eluted, and the fractions containing this material were combined and evaporated to give the pure title compound, (0.21 g), confirmed spectroscopically to be identical to the product of Example 4. EXAMPLE 50 Preparation of l-carbamoyl-2-(2,4-dichlorophenyl)-3-(lH-1,2,4- triazol-l-yl)propan-2-ol EMI55.1 1-Cyano-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol-1yl)propan-2-ol (1.0 g) was heated at 600 for 2 1/2 hours in sulphuric acid (10 ml, 80%). The reaction mixture was then cooled to room temperature, carefully treated with water (100 ml), and adjusted to pH9 with solid sodium hydroxide. The resulting solution was extracted with methylene chloride (3 x 50 ml), and the combined extracts were evaporated to a gum, which was chromatographed on silica ("Kieselgel 60", Merck), eluting-with methylene chloride containing 3Z by volume methanol, increasing to 6% methanol. The fractions which contained the product as judged by t.l.c. were combined- and evaporated to a white solid, 0.91 g. This was dissolved in a mixture of acetone and methylene chloride at reflux and the product was precipitated by the addition of hexane to give fine crystals, m.p. 144-145.5, 0.61 g, confirmed spectroscopically to be identical with. the product of Example 4 after drying under vacuum for 7 hours at 800. EXAMPLE 51 Preparation of 2-(2,4-dichlorophenyl)-1-{N-(2-[methylsulphinyl) ethyl)carbamoyl}-3-(1H-1,2,4-triazol-1-yl)propan-2-ol EMI56.1 2-(2,4-Dichlorophenyl)-1-{N-(2-[methylthio]ethyl)carbamoyl} -3-(1H-1,2,4-triazol-1-yl)propan-2-ol (0.8 g) and m-chloroperbenzoic acid (85%, 0.35 g, I equiv.) were stirred at room temperature in a mixture of isopropanol. and methylene chloride (1:1, v/v, 40 ml) for two days. The solvents were then removed under reduced pressure, and -the residue was chromatographed on silica (Merck, "Kieselgel 60", 25 g), eluting with a mixture of chloroform, methanol and ammonia (S.G. 0.880) (160:20:5, v/v). A portion of the isomer which was eluted first was obtained pure, 116 mg, m.p. 168-1700. Analysis %: Found: C,44.1; H,4.4; N,13.5 C15H18Cl2N4O35 requires: C,44.4; H,4.5; N,13.8. The bulk of the material eluted as a mixture containing both diastereoisomers (330 mg). This material was used in the preparation of the sulphone which follows. EXAMPLE 52 Preparation of 2-(2,4-dichlorophenyl)-1- N-(2-(methylsulphonyl] ethyl)carbamoyl}-3-(1H-1,2,4-triazol-1-yl)propan-2-ol EMI57.1 The unseparated mixture of diastereoisomers from the previous Example (330 mg) and m-chloroperbenzoic acid (140 mg) were stirred together in a mixture of isopropanol and methylene chloride (1:1, v[v, 20 ml) at 00. After one hour at 00, the reaction mixture was allowed to reach room temperature and was stirred overnight. The solvents were then removed under reduced pressure, and the residue was dissolved in ethyl acetate (50 ml). The resulting solution was washed with saturated sodium bicarbonate solution (2 x 20 ml), then with saturated sodium chloride solution (2 x 20 ml), dried (MgSO4), and evaporated to a gum which was triturated with diisopropyl ether to give a white solid, 209 mg, m.p. 123-124 . Analysis %: Found: C,42.6; H,4.3; ,13.2 C15H18Cl2N4O4S requires: C,42.8; H,4.3; N,l3.3. EXAMPLE 53 Preparation of l-azlrldnylcarbonyl-2-(2,4-dichlorophenyl)-3-(La- 1,2,4-triazol-1-yl)propan-2-ol EMI59.1 l-(N-{2-Eydroxyethyl]carbamoyl)-2-(2,4-dichlorophenyl)-3- (1H-1,2,4-triazol-1-yl)propan-2-ol (1.0 g), triphenylphosphine (1.09 g) and diethyl azodicarboxylate (0.72 g) were stirred at room temperature for 20 hours in dry tetrahydrofuran (20 ml). The reaction mixture was then diluted with water (70 ml) and extracted with ethyl acetate- (3 x 50 ml). The combined organic extracts were washed with saturated sodium- chloride solution (2 x 20 ml), dried (MgSO4), and evaporated to a brown gum. This material was chromatographed on Merck "Kieselgel 60" silica, eluting with 5% by volume ethanol in ether increasing to 10% ethanol in ether. The eluted material, which was one compound as judged by t.l.c., was crystallised from ethyl acetate/n-pentane to give colourless crystals of the title compound, 441 mg, m.p. 151-153 . Analysis %: Found: C,49.2; E.4.Q; N, 16.3 C14H14Cl2N4O2 requires: C,49.3; H,4.1; N,16.4. EXAMPLE 54 Preparation of 2-(2,4-dichlorophenyl)-1-thiocarbamoyl-3-(1H-1,2,4 triazol-l-yl)propan-2-ol EMI60.1 A mixture of 3-cyano-2-(2,4-dichlorophenyl)-1-(1H-1,2,4- triazol-l-yl)propan-2-oL (250 mg), O,O-diethyl dithiophosphoric acid (0.5 ml) and water (0.1 ml) was heated on steam bath for 3 hours, evaporated under reduced pressure to an oil, and chromatographed on "Merck 60H" (Trademark) silica (10 g) eluting with ethyl acetate. The eluted product, after evaporation, was triturated under petroleum ether (b.p. 40-60") to give the title compound as a yellow solid (143 mg), m.p. 1580-1590. Analysis Z:- Found: C,43.8; H,3.6; N,16.9 Calculated for C12H12Cl2N4OS: C,43.6; H,3.6; N,16.9. EXAMPLE 55 The following illustrate pharmaceutical compositions for the treatment of fungal infections: (a) Capsule: 71 parts by weight of the compound of Example I or 2 are granulated with 3 parts maize starch and 22 parts lactose and then a further 3 parts maize starch and 1 part magnesium stearate are added. The mixture is regranulated and filled into hard gelatin capsules. (b) Cream: 2 parts by weight of the compound of Example 3 are dissolved- in 10 parts of propylene glycol and mixed into 88 parts of a. vanishing cream base. (c) Pessary: 2 parts by weight of the compound of Example 5 are suspended in 98 parts of a warm liquified suppository base which is poured into moulds and allowed to solidify. The following Preparations, in which all temperatures are in C, illustrate the preparation of certain starting materials: Preparation 1 Preparation of l-carboxy-2-( 2 ,4-dichlorophenyl) -3-(1E-1, Z, 4- triazol-1-yl)propan-2-ol EMI62.1 1-Cyano-2-(2,4-Dichlorophenyl)-3-(1H-1,2,4-triazol-1-yl)-2propanol (4 g, 13.9 mmole) was dissolved in 40% aqueous sulphuric acid (100 ml) and heated in an oil bath at 100-110 for 18 hours. The solution was then cooled, diluted with water (200 ml), and rendered alkaline by the slow addition of solid sodium bicarbonate. The mixture was then extracted several times with ethyl acetate (3 x 100 ml.) and the aqueous phase was rendered acidic (pH3) by the addition of dilute orthophosphoric acid. The aqueous phase was then extracted with ether (3 x 150 ml), the combined ether extracts were washed once with water, and then dried over magnesium sulphate. Evaporation of the ether gave the title compound as a pale yellow solid, 2.7 g, (62%), m.p. 158-94. Analysis Z:- Found: C,46.35; ,3.5 ; N,13.6 Required for C12H11Cl2N3O3: C,45.6 ; H,3.5 ; N,13.3. Also prepared similarly was l-carboxy-2-(2,4-difluorophenyl)-3-(1H-1,2,4-triazol-1-yl)propan-2-ol, m.p. 185-7 . Analysis %: Found: C,50.8; H,3.9; N,14.8 Calculated for C12H11F2N3O3: C,51.0; H,3.9; N,14.8. Preparation 2 Preparation of 3-(2,4-dichlorophenyl)-3-hydroxy-4-(1E-1,2,4- triazol-l-yl)butyrimidic acid, ethyl ester dihydrochioride EMI63.1 l-Cyano-2-(2,4-dichlorophenyl)-3-(lH-1,2,4-triazol-1-yl) propan-2-ol (1 g) was dissolved in dry ethyl alcohol (100 ml) and dry hydrogen chloride gas was bubbled in, at 00, for 10 minutes. The reaction mixture was then stirred at room temperature overnight, and then the solvent was decanted from the solid. The solid was then-washed with dry ether and dried to yield the title compour (1.15 g), m.p. 154-156 . The product was used in Example 4. Analysis %: Found: C,40.6; H,4.4; N,13.6 Calculated for C14H16Cl2N4O2.2HCl: C,40.4; H,4.4; N,13.5. Preparation 3 (i) Preparation of 2-(1H-1,2,4-Triazol-1-yl)-2',4'-dichloro acetophenone (y) This compound was prepared similarly to the method described. in British Patent Specification No. 1512918: EMI64.1 (ii) Preparation of 2-(2,4-Dichlorophenyl)-2-(1H-1,2,4-triazol1-ylmethyl)-oxirane (Z) EMI64.2 3.78 g (0.079 Mole) of sodium hydride (50% dispersion in oil) was suspended, with stirring, in 20 ml of dry ether The ether was then removed by decantation, and the sodium hydride was dried in a stream of dry nitrogen. 100 MI of dry dimethyl sulphoxide was added followed by 17.34 g (0.079 mole) of dry powdered trimethylsulphoxonium iodide, in portions, over 15 minutes. The resulting mixture- was stirred for 30 minutes at room temperature (20 C). 18.33 g (0.072 Mole) of compound (Y) as a solution in 50 ml. of dry dimethyl sulphoxide was then added. The mature was heated at 600C for 3 hours and then stood at room temperature overnight, The reaction mixture was cooled and quenched in ice. The product was then extracted into ethyl acetate (600 ml). The ethyl. acetate layer was separated, dried over magnesium sulphate, and concer.trated. to give a red gum. Column chromatography of the gum on: silica, eluting with ether, gave the product (z). On evaporation, 6.62 g (34.4%) of the title compound (z) was obtained as a gum which solidified on trituration. The pure product melted at 57-59 Analysis %: Found: C,48.6; H,3.3; N,15.3 Calculated for CllN C12N30: C,49.O; fl,3.4; N,l5.5. Preparation 4 (A) Preparation of 2-chloro-2',4'-difluoroacetophenone EMI66.1 Chloroacetyl chloride (113 g, 1.0 M) was added dropwise to a stirred mixture of 1,3-difluorobenzene (114 g, 1.0 M) and anhydrous aluminium chloride (146.6 g, 1.1 M) at room temperature (20QC). The mixture was stirred for a further five hours at 50-55 C. Methylene chloride (48.5 ml) was added slowly as the mixture was allowed to cool to room temperature. The methylene chloride layer was separated, washed with water (2 x 320 ml) and the solvent removed by distillation at reduced pressure leaving a pale yellow solid (180 g). A portion of the crude product (145 g) was crystallised from n-hexane (435 ml) giving the title compound (113 g, 73%) m.pt. 470-490C (literature* 46.50C). IR (KBr) and nmr (CDC13) were consistent with the desired structure. * Von D. Ehlers, H. Bercher and A. Grisk, J. Prakt, Chem., 315, 1169 (1973). (B) Preparation of 2',4'-difluoro-2-(1H-1,2,4-triazol-1-yl) acetophenone hydrochloride EMI67.1 To a mixture of 1,2,4-triazole (30.4 g, 0.44 M) and triethylamine (15.1 g, 0.15 M) in refluxing ethyl acetate (186 ml) was added a solution of 2-chloro-2',4'-difluoroacetophenone (38.1 g; 0.2 M) in ethyl acetate (80 ml), The mixture was refluxed for six hours then cooled to room temperature and the insolubles were removed by filtration. The filtrate was washed with water (2 x 200 ml) and then the solvent was removed by distillation at reduced pressure. The crude product was dissolved in ethyl acetate (150 ml) then 25% w/v RCl gas in isopropanol was added. The mixture was granulated at OOC for one hour and then the solid was collected by filtration and dried to give the title compound (21.6 g, 40%), melting point 167"-1700C. IR (K8r) and nmr (DM50) were consistent with the desired structure. This intermediate was characterised as the free base, which was prepared by the following technique: To a stirred slurry of sodium bicarbonate (16.8 g, 0.2 M) and 1,2,4-triazole (27.6 g, 0.4 M) in refluxing toluene (180 ml) was added a solution of 2-chloro-2',4'-difluoroacetophenone (38.1 g, 0.2 M) in toluene (45 ml). The mixture was stirred at reflux for three hours and the water formed during the reaction was removed using a Dean and Stark trap. The reaction mixture was cooled to room temperature and then water (180 ml) was added. The toluene layer was separated and the solvent removed by distillation at reduced pressure. The resulting pale brown solid was crystallised from 1:1 ethyl acetate: n-hexane (70 ml) giving the title compound (3.9 g), melting point 103 -105 C. The IR (KBr) and nmr (CDC13) were consistent with the desired structure. Analysis z:- Found: C,53.6 ; H,3.15; N,18.? Calculated for C10H7F2N3O: C,53.8; H,3.2; N,18.8 For 4'-chloro-2-(1H-1,2,4-triazol-1-yl) acetophenone see DT-OS 2431407. Activity Data In mice, PD50 (oral) values in mg./kg. Product of Example No. PD50 (mg./kg.) 1 1.3 2 1.3 3 ¯40 4 0.2 5 0.2 6 - 20 7 0.4 8 0.1 9 0.4 10 0.1 11 ¯30 12 3.1 13 ¯40 14 0.4 15 0.2 16 ¯20 17 1.5 18 0.4 19 2.2 20 0.1 21 0.6 22 0.2 23 0.2 24 0.4 Product of Example No. PD50 (mg./kg.) 25 3.5. 26 2.2 27 4.2 28 4.2 29 4.2 30 0.1 31 3.1 32 0.2 33 0.3 34 0.1 35 2.0 36 10 37 0.4 38 0.4 39 0.5 40 0.2 41 3.1 42 0.6 43(B) 0.2 44 0.2 45 0.1 46 0.1 47 0.1 48 ¯0.3 49 0.2 50 0.2 Product of Example No. PD50 (mg./kg.) 51 (isomer melting at 0.2 168-170%) 52 0.1 53 4.2 54 0.5

Claims: CLAMS 1. Compounds of the formula: EMI72.1 where R is phenyl optionally substituted by I to 3 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; RÚ is -CN, -CSNH2, or -CONRêR where either (a) Rê is H or C1-C6 alkyl, and R3 is H, C1-C6 alkyl, benzyl, phenethyl, phenyl, -CH2CF3, adamantyl, pyridylmethyl, C3-C7 cycloalkyl, carbamoylmethyl, (C2-C4 alkenyl)methyl, 2-hydroxyethyl, 2-(dimethylamino)ethyl, 2-(methylthio)ethyl, 2-(methylsulphinyl)ethyl, 2-(methylsulphonyl)ethyl or 2-phenoxyethyl; said benzyl, phenethyl, phenyl and phenoxy group being optionally ringsubstituted by 1 or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, C1, Br, I and CF3, (b) R2 and R3 taken together with the nitrogen atom to which they are attached represent a group of the formula: EMI72.2 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; (c) Rê is H or C1-C4 alkyl and R is C2-C4 alkanoyl or benzoyl, said benzoyl group being optionally substituted by 1 or 2 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy; or (d) Rê and R3 are both (C1-C4 alkoxy)carbonyl; R5 is H or CH3; and R6 is H or CR3; and their pharmaceutically acceptable salts. 2 A compound as claimed in claim 1 wherein R is phenyl substituted by I to 3 substituents each independently selected from F, CI, Br, I and CF3. 3. A compound as claimed in claim 2, wherein R is 4-fluorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-iodophenyl, 4-trifluoromethylphenyl, 2-chlorophenyl, 2 ,4-dichlorophenyl, 2,4-difluorophenyl, 2,5-difluorophenyl, 2-fluoro-4-chlorophenyl, 2-chloro-4-fluorophenyl, 2,4,6-trifluorophenyl and 4-bromo-2,5-difluorophenyl. 4. A compound as claimed in claim 3, wherein R is 2, 4-dichlorophenyl, 2,4-difluorophenyl or 4-chlorophenyl. 5. A compound as claimed in claim 4, wherein R is 2, 4-dichlorophenyl or 2,4-difluorophenyl. 6. A compound as claimed in any one of the preceding claims wherein R1 is -CN, -CSNH2, or -CONRêR wherein either (a) R2 is H, CH3 or C2H5, and R is H, C1-C6 alkyl, p-chlorobenzyl, p-chlorophenethyl, p-methylphenethyl, -CR2 CF3, 1-adamantyl, 4-pyridylmethyl, cyclopropyl, carbamoylmethyl, -CH2.CH=CH2, 2-hydroxyethyl, 2-(dimethylamino)ethyl, 2-(methylthio)ethyl, 2-(methylsulphinylethyl), 2-(methylsulphonylethvl), p-chlorophenyl, or 2-(p-chlorophenoxy)ethyl; (b) R2 and R3 taken together with the nitrogen atom to which they are attached represent a group of the formula: EMI74.1 (c) R2 is H and R3 is acetyl, propionyl or p-chlorobenzoyl; or (d) Rê and R are both -COOCH3 7. A compound as claimed in claim 1 of the formula: EMI74.2 where R is phenyl optionally substituted by 1 to 3 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; and K1 is -CN or -CONRêR where R2 is H or C1-C6 alkyl, and R3 is H, C1-C6 alkyl, benzyl, -CH2CF3 or adamantyl, said benzyl group being optionally substituted on its phenyl ring portion by 1 or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, Cl, Br, I and CF3, or R2 and R taken together with the nitrogen atom to which they are attached represent a group of the formula EMI75.1 where K4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; and their pharmaceutically acceptable salts. 8. A compound as claimed in claim I of the formula: EMI75.2 where R is phenyl optionally substituted by 1 to 3 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4, alkoxy, or R is a 5-chloropyrid-2-yl group; ; R1 is -CN or -CONRêR where R2 is H or C1-C6 alkyl, and R3 is H, C1-C6 alkyl, benzyl, -CH2CF3 or adamantyl, said benzyl group being optionally substituted on its phenyl ring portion by 1 or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, C1, Br, I and CF3, or K2 and R3 taken together with the nitrogen atom to which they are attached represent a group of the formula EMI76.1 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; and R5 is H or CH3; and their pharmaceutically acceptable salts. 9. A compound as claimed in claim 1 of the formula: EMI76.2 where R is a phenyl group optionally substituted by I to 3 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or K-is a 5-chloropyrid-2-yl group; R5 and R6 are each independently H or CH3; and either (a) R2 is H or C1-C4 alkyl and R3 is C2-C4 alkanoyl or benzoyl, said benzoyl group being optionally substituted by 1 or 2 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy; or (b) R2 and R are both (C1-C4 alkoxy)carbonyl; and their pharmaceutically and agriculturally acceptable salts. 10. A compound of the formula (I) as claimed in any one of claims I to 8 wherein K1 is -CONR2 or -CONH(C1-C4 alkyl). 11. A compound of the formula (I) as claimed in any one of claims 1 to 6, 8 and 9 wherein R5 and R6 are E. 12. A compound of the formula (I) as claimed in claim 1 wherein R is 2,4-dichlorophenyl or 2,4-difluorophenyl, R5 and K6 are H, and * is -CONH2, -CONH.CH3 or -CONHC2H3. 13. An agriculturally acceptable salt of a compound of the formula (I) as claimed in any one of the preceding claims. 14. A pharmaceutical composition comprising a compound of formula (I) as claimed in any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, together with apharmaceutically acceptable diluent or carrier. 15. A compound of the formula (I) as defined in any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, for use in medicine, particularly for use as an antifungal agent. 16. A composition for use as an agricultural fungicide comprising a compound of the formula (I) as claimed in any one of claims 1 to 12, or an agricuturally acceptable salt thereof, together with an agriculturally acceptable diluent or carrier. 17. A method of treating a plant or seed having a fungal infection, which comprises contacting said plant, seed or locus of said plant with an antifungally effective amount of a compound of the formula (I) as claimed in any one of claims I to 12, salt as claimed in claim 13, or composition as claimed in claim 16. 18. A compound of the formula:- EMI78.1 where K, R5 and R6 are as defined in claim 1. CLAIMS FOR AUSTRIA 1. A process for preparing a compound of the formula: EMI79.1 where R is phenyl optionally substituted by 1 to 3 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy: or 5-chloropyrid-2-yl; characterised by reacting a compound of the formula: EMI79.2 with cyanide ions. 2. A process according to claim 1, characterised in that said cyanide ions are provided by sodium or potassium cyanide. 3. A process for preparing a compound of the formula: EMI79.3 where R is as defined in claim 1, and R5 and R6 are each H or CH3, characterised by reacting a compound of the formula: EMI80.1 where R is as defined above, firstly with an anion of the formula:- EMI80.2 where R5 and R6 are- as defined above, and then with acid. 4. A process according to claim 3, characterised in that the anion is generated by reacting a nitrile of the formula: EMI80.3 where R5 and R6 are as defined in claim 3, with n-butyllithium. 5. A process for preparing a compound of the formula: EMI81.1 where R is as defined in claim I, characterised in that a compound of the formula: EMI81.2 is reacted with an acid. 6. A process according to claim 5, characterised in that the acid is supplied by using compound (IV) in acid addition salt form, and wherein the reaction is carried by heating the reactants in-an organic solvent at up to 1800C. 7. A process for preparing a compound of the formula: EMI81.3 where R is as defined in claim 1, R5 and R6 are each H or CR3, and either (a) Rê is H or C1-C6 alkyl, and R3 is H, C1C6 alkyl, benzyl, phenethyl, phenyl, -CH2-CF3, adamantyl, pyridylmethyl, C3-C7 cycloalkyl, carbamoylmethyl, (C2-C4 alkenyl)methyl, 2-hydroxyethyl, 2- (dimethylamino) ethyl, 2-(methylthio) ethyl, 2-(methylsulphinyl)ethyl, 2-(methylsulphonyl)ethyl or 2-phenoxyethyl; said benzyl, phenethyl, phenyl and phenoxy groups being optionally ring-substituted by 1 or 2 substituents each independently selected from C14 alkyl, C1-C4 alkoxy, F, Cl, Br, I and CF3, or (b) Rê and K3 taken together with the nitrogen atoa to which they are attached represent a group of the formula: EMI82.1 EMI82.2 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; characterised by reacting a compound of the formula: EMI82.3 or an acylating derivative thereof, where R, R5 and R6 are as defined above, with a compound of the formula: R2R3NE where R2 and R3 are as defined above. 8. A process- according to claim 7, characterised in that said acylating derivative is an acid chloride or bromide, mixed anhydride, or C -C alkyl, succinimido, phthalimido or benzotriazol-l-yl ester of the acid (V). 9. A process for preparing a compound of the formula: EMI83.1 where R1 is -CONH(C1-C4 alkyl) or -CON(Cl-C4 alkyl)2, and R, R5 and R are as defined in claim 3, characterised by alkylating the corresponding compound in which R is -CONH2. 10. A process according to claim 9, characterised in that the alkylation is carried out using the appropriate quantity of a C1-C4 alkyl iodide or bromide in the presence of a base. 11. A process for preparing a compound of the formula: EMI84.1 where R, R5 and R are as defined in claim 3, characterised by the controlled hydrolysis of a compound of the formula: - EMI84.2 where R, R5 and R6 are as defined above. 12. A process according to claim 11, characterised in that the hydrolysis is carried out using aqueous sulphuric acid at-up to 100 C. 13. A process for preparing a compound of the formula: EMI84.3 where R, * , R , R5 and R6 are as defined in claim 7, characterised by reacting a compound of the formula: EMI85.1 where R, R5 and R6 are as defined above, with an amine of the formula RêR NH where K2 and R3 are as defined above. 14. A process according to claim 13, characterised in that the reaction is carried out in ethanol at about room temperature. 15. A process for preparing a compound of the formula: EMI85.2 where R is as defined in claim 1, R5 and R6 are each H or CH3, Rê is H or C1-C4 alkyl, and R is C2-C4 alkanoyl or benzoyl, said benzoyl group being optionally substituted by 1 or 2 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C -C alkoxy, characterised by reacting a compound of the formula: EMI86.1 where R, R2, K and R6 are as defined above, with an acid halide or anhydride of the formula: R .X or (R3)2.0 where R3 is as defined above and X is Cl or Br. 16. A process for preparing a compound of the formula: EMI86.2 where R, R5 and R6 are as defined in claim 3, characterised by reacting a compound of the formula: EMI86.3 with at least 2 equivalents of an alkyl haloformate of the formula: - EMI87.1 where X is Cl or Br. 17. A process for preparing a compound of the formula EMI87.2 where R, R5 and R6 are as defined in claim 3, characterised by reacting a compound of the formula: EMI87.3 where R, R5 and R6 are as defined above, with a compound of the formula: EMI88.1 in the presence of water. 18. A process for preparing a compound of the formula EMI88.2 where R is as defined in claim 1, characterised by reacting a compound of the formula: EMI88.3 where R is as defined above, firstly with the product of the reaction of n-butyllithium and bis(trimethylsilyl)acetamide, and then with an acid 19. A process for preparing a compound of the formula: EMI89.1 where R is as defined in claim 1, characterised by reacting a compound of the formula: EMI89.2 where R is as defined above, with triphenylphosphine and diethyl azodicarboxylate. 20. A process according to any one of the preceding claims, characterised in that the product of the reaction is converted into a pharmaceutically or agriculturally acceptable acid addition salt by reaction with a suitable acid. 21. A process for preparing a lactone of the formula: EMI89.3 where R, K5 and K6 are as defined in claim 3, characterised by cyclising a compound of the formula: EMI90.1 where Q is C1-C4 alkyl, phthalimido, succinimido or l-benzotriazolyl. 22. A process according to claim 21, where said cyclisation is carried out using a ter-tiary amine base, an alkali metal hydride or zinc. 23. The use of the compounds of the following formula, and their agriculturally acceptable salts, as agricultural fungicides: EMI90.2 where R is phenyl optionally substituted by 1 to 3 substituents each independently selected from F, C1, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy, or R is a 5-chloropyrid-2-yl group; RÚ is -CN, -CSNH2, or -CONRêR where either (a) Rê is H or C1-C alkyl, and R3 is H, C1-C6 alkyl, benzyl, phenethyl, phenyl, -CH2CF3, adamantyl, pyridylmethyl, C3-C7 cycloalkyl, carbamoyl methyl, (C2-C4 alkenyl)methyl, 2-hydroxyethyl, 2-(dimethylamino)ethyl, 2-(methylthio)ethyl, 2-(methylsulphinyl) ethyl, 2-(methylsulphonyl)ethyl or 2-phenoxyethyl; said benzyl, phenethyl, phenyl and phenoxy group being optionally ringsubstituted by 1 or 2 substituents each independently selected from C1-C4 alkyl, C1-C4 alkoxy, F, Cl, Br, I and CF3, (b) R and 3 R3 taken together with the nitrogen atom to which they are attached represent a group of the formula: EMI91.1 where R4 is H, C1-C4 alkyl, C2-C4 alkanoyl or (C1-C4 alkoxy)carbonyl; (c) Rê is H or C1-C4 alkyl and R3 is C2-C4 alkanoyl or benzoyl, said benzoyl group being optionally substituted by 1 or 2 substituents each independently selected from F, Cl, Br, I, CF3, C1-C4 alkyl and C1-C4 alkoxy; or (d) Rê and -K3 are both CC -C alkoxy)carbonyl; R5 is H or CH3; and R6 is H or CH3.

Inventors: COOPER, KELVIN, DR., GYMER, GEOFFREY EDWARD, DR., RICHARDSON, KENNETH, DR.
Applicants: PFIZER CORPORATION, PFIZER LIMITED
Application year: 1983
Main classification: C07D249
IPC codes: A01N43, C07D249, C07C45, C07D521