SPECIMEN MOUNTING ADHESIVE COMPOSITION

Abstract: A curable adhesive composition transferring and adher­ ing tape-mounted histological sections, to specimen mount­ ing surfaces, usually glass microscope slides, to expedite and simplify the safe removal of the mounting tape and the subsequent processing of the slide-mounted section, com­ prises a polymeric mixture which has high tack prior to curing, is substantially non-diffusable and non-flowable into tissue sections and, after curing, has a refractive index substantially similar to that of the specimen section and is non-labile to conventional histological solvents and stains. A preferred composition comprises diacrylate-terminated polyurethane, a diacrylate ester of an eposy resin and a diethoxyacetophenone initiator. It is usually formed onto the mounting surface as a solution in a conventional organic solvents, such as isopropanol or toluene.

Description: "SPECIMEN MOUNTING ADHESIVE COMPOSITION" This invention relates to curable adhesive compositions useful for biological specimen mounting. Very thin slices of animal and plant tissue are prepared for many different kinds of microscopic studies by sectioning with a variety of kinds of microtomes. The tissue may be cut fresh, but the soft and compliant nature of most fresh tissue makes it very difficult to cut undistorted thin sections. If the tissue is frozen and is cut on a freezing microtome or in a cryostat at temperatures below OOC (320F), the hardness of the frozen water within the tissue permits sections to be cut relatively easily as thin as a few micrometers. However, these sections are brittle and friable and therefore difficult to handle and process further. To make sectioning of tissue still easier, a number of procedures produce a block of supported tissue which has superior sectioning properties and from which high quality, relatively easy-to-handle tissue sections can be made. Such procedures typically involve: (1) fixation of the tissue in a solution which insolubilises the natural polymers of which tissue cells are composed, and which hardens them; (2) dehydration through a series of water-miscible (e.g. an alcohol) and then paraffin or plastic-monomer-miscible (e.g. toluene or xylene) solvents; (3) infiltration in melted paraffin or monomer solution; and (4) embedding by freezing the paraffin or polymerising the monomer to form a solid polymer. [Staining Methods, J.F.S. McManus and R.W. Mowry (P.B. Hoeber, Inc., N.Y. 1960); Techniques for Electron Microscopy, D. Kay, Ed. (Blackwell Sci. Publ., Oxford, England, 1965) pp. 166-212.3 However, there are occasional specimens which remain difficult to section. As the section is cut, the parts of the cut section fragment and fall from the cut section, or fall from the section as it is removed from the microtome A. Palmgren, Nature, Vol. 174, p. 46 (1954), introduced the use of a conventional adhesive tape as a sectioning aid for the cutting of very large, hard or brittle specimens. A piece of adhesive tape is applied to the surface of a cut frozen or paraffin block, JUSt before the specimen is advanced the incremental thickness of a (next) section. The microtome advances and the next section is cut and is thus supported by the applied tape. The quality of the uncompressed section of hard brittle and friable tissue produced by this means can be far superior to that of a conventional section of the same block of tissue. However, following Palmgren, processing such a section while it remains on the tape, or transferring it to a glass slide (to permit it to be processed thereafter in a conventional way) involved elaborate, time-consuming and inconvenient methods, which are also potentially damaging to the section. Palmgren's method, therefore, did not become popular. W. E. Beckel, Nature, Vol. 184, p. 1584 (1959) introduced the use of Scotch brand No. 810 celluloseacetate-backed adhesive tape in Palmgren's process. The tape-mounted sections were applied, section-side down on conventionally wet albuminised glass slides. After thorough drying for a few hours, the adhesive backing, the adhesive layer and the paraffin were all dissolved in tetrahydrofuran in 30 minutes, leaving the section adhering to the glass slide-, and available for further processing by conventional techniques. Alternatively, chloroform for 2 minutes, followed by xylene for 30 minutes, worked equally well. Beckel also described a "more rapid method which used a film of albumin and a solution of 2 per cent celloidin in methyl benzoate or ethyl alcohol to "cement" the section to the glass slide followed by 1 minute in chloroform and 10 minutes in xylene to complete the treatment. Some other equally time-consuming variations were described. D. S. Gowers and R. E. Miller, Nature, Vol. 190, p. 425 (1961), attempted to repeat Beckel's method but found that, with available Scotch brand No. 810 tape, the adhesive could not be dissolved, and with the best alternative available tape, Tuck brand No. 200, safe removal of the tape without damaging the section in solvent took from 1 to 10 hours. R. P. Wedeen and H. I. Jernow, Am. J. Physiol., Vol. 214, p. 776 (1968) used cyanoacrylate (Eastman 910 "super-glue") to attach adhesive-tape-supported frozen sections to radioautographic (photographic) plates. The cyanoacrylate is initially liquid, but polymerises to a solid when it is squeezed out into a thin film. The cyanoacrylate polymer is soluble in xylene and other processing solvents, which would cause the section to float free, and so is not useful for ordinary use. In another area of study relating to the field of specimen section handling, various resinous mounting media have been developed. After the tissue section has been stained, the slide is transferred from water through alcoholic and then resin-compatible solvents, such as xylene, and the section is mounted in a resinous medium chosen to have a refractive index which closely approximates the average refractive index of the unstained tissue specimens, e.g. a refractive index from about 1.530 to about 1.570. Such are described, for example, in U.S. Patent No. 14,120,991, and McManus & Mowry, above. We have now devised a curable adhesive composition for transferring and mounting a tape-supported tissue specimen onto a specimen support whereby certain disadvantages of prior art procedures are reduced or overcome. According to the invention, there is provided a curable adhesive composition for transferring and mounting a tape-supported histological tissue section onto a specimen support for further processing, comprising at least one polymerisable component and a polymerisation curing initiator therefor and which composition has the following characteristics: : a. is a high tack pressure-sensitive adhesive prior to cure; b. has a sufficiently low diffusion coefficient before cure to substantially prevent diffusion into tissue; c. has a viscosity which is sufficiently high before cure to substantially prevent flow into tissue during specimen application to said specimen support; d. is rapidly cured; e. is substantially non-swelling, tack-free, insoluble, unreactive and non-ionic, after cure; f. has a cured refractive index between about 1.53 and about 1.56; and g. after cure, bonds strongly to both the specimen surface and support surface. In use, a curable adhesive composition of the invention is typically coated onto a glass microscope slide. A cut paraffin embedded section, supported on adhesive tape, is pressed, section-side down, onto the adhesivecoated glass microscope slide. The contact is ensured by smoothing with moderate finger pressure. The laminate, bottom-of-the-glass-side up, is placed within a few inches of a 4-watt black-light fluorescent lamp with a phosphor peak-light output near 350 nm, for 3 minutes. The laminate is then immersed in a solvent, such as xylene, and the adhesive tape is gently peeled away over a period of about 5 seconds. The very strong bond between the bottom of the section and the glass slide make this otherwise critical step very easy. The solvent greatly weakens the tapeadhesive-to-tissue and the tape-adhesive-to-tape-backing bonds. Any remaining adhesive layer from the tape and the paraffin are dissolved away in about one minute with gentle agitation, and the slides ready for processing in the conventional manner. However, for a detail description of the method, reference is made to our copending European patent application no. (claiming priority from U.S. application no. 417307) filed on even date herewith. In a preferred embodiment, the composition of the present invention is a curable polymeric mixture which has the high tack of a pressure-sensitive adhesive prior to curing, is substantially non-diffusable into tissue sections and, after curing, has a refractive index substantially similar to that of the tissue section and is non-labile to conventional histological solvents and stains. A preferred composition comprises a diacrylate-terminated polyurethane, a diacrylate ester of an epoxy resin and a diethoxyacetophenone initiator. It is usually formed onto the mounting surface of the specimen support as a solution in a conventinal organic solvent, such as toluene or isopropanol. The preferred composition suitable to achieve the above objectives comprises a mixture of high molecular weight inter-reative oligomers. The high molecular weight ensures both a high viscosity and a low diffusion coefficient. The upper limitation of the molecular weight is such that there remains high tack in the precured mixture when coated on a mounting surface In one preferred embodiment, the mixture includes a first oligomer having a cured refractive index above 1.560 and another that has a cured refractive index less than 1.530, for example, an oligomeric diacrylate ester of an epoxy resin and a polyurethane diacylate oligomer, respectively. Both cured resins must be resistance to histological stains and solvents. Preferably, both resins are provided with terminal acryl or methacryl groups to permit radical initiated cross-linked polymerisation. These oligomer resins are mixed in proportions, more fully described below, which provide a cured refractive index between about 1.530 and about 1.560, using the Dale-Gladstone equation described in standard physical chemistry texts and procedures. Components particularly preferred in forming the composition of the invention include those of low refractive index such as diacrylate-terminated polyurethanes, including Purelast 190, 186 and 169, available from Polymer Systems Corporation, Little Falls, New Jersey, in combination with those of high refractive index such as diacrylate esters of epoxy resins, such as Epicryl 3 resin and Shell Developmental Polyester Resin DRH 301.1, both available from Shell Corporation, a division of Shell Oil Company, Houston, Texas. A further essential component of the composition is an initiator, such as a thermal initiator or preferably a photoinitiator. A thermal initiator, such as benzoyl peroxide, acetylperoxide or azoisobutyronitrile may be included, if curing is to be effected thermally. When cured in this manner, it is preferable to add the initiator just prior to curing. The thermal treatment is effectively achieved by heating at a temperature from about 1400C to about 200 0C. Alternatively, a broad spectrum of photoinitiators can be used including benzoin and acetophenones such as 2 , 2-dimethoxy-2-phenylacetophenone and 2,2-diethoxyacetophenone. The preferred photoinitiator for use with the above polymer mixture is 2,2-diethoxyacetophenone, available from the Upjohn Company, Fine Chemical Division, New Haven, Connecticut. Toluene and isopropanol are standard commercially available reagent grade solvents. Typical proportions of the epoxy ester to the urethane resin is from about 2:1 to about 3:1 by weight. The ratio of the combined resins to the initiator component is approximately 20:1 by weight. About 0.5 grams per millilitreoftflemixtureof the above two resins in an appropriate solvent is preferred. Other combinations of reactive oligomer and/or polymer systems such as yield epoxy resins, polyurethanes, polyesters, etc., with other initiators and solvents can be formulated by those skilled in the art to fill the requirements in the -listed specifications. The compositions of the invention are suitable for layering on specimen supports such as glass microscope slides. They can, for example, be layered by printing, spraying or spreading in film-forming-solvents which thereafter evaporate. In one preferred embodiment, the composition is coated by hand, using a blade coating device, or by machine, using techniques such as dip or bead coating. If machine coating is used, hopper coating techniques, such as are known in the photographic industry, are preferred. The thickness of any layer and its degree of permeability are widely variable and dependent on actual use. Dry thickness of the adhesive prior to curing is from about 10 to about 25 micrometers.

Claims: CLAIMS: 1. A curable adhesive composition for transferring and mounting a tape-supported histological tissue section onto a specimen support for further processing, comprising at least one polymerisable component and a polymerisation curing initiator therefor and which composition has the following characteristics: a. is a high tack pressure-sensitive adhesive prior to cure; b. has a sufficiently low diffusion coefficient before cure to substantially prevent diffusion into tissue; c. has a viscosity which is sufficiently high before cure to substantially prevent flow into tissue during specimen application to said specimen support; d. is rapidly cured; e. is substantially non-swelling, tack-free, insoluble, unreactive and non-ionic, after cure; f. has a cured refractive index between about 1.53 and about 1.56; and g. after cure, bonds strongly to both the specimen surface and support surface. 2. A composition according to claim 1, wherein said polymerisable component comprises at least one difunctional oligomer. 3. A composition according to claim 2, which comprises a mixture of oligomers, at least one of which has a low refractive index, preferably a cured refractive index less than about 1.530, and at least one of which has a high refractive index, preferably a cured refractive index above about 1.560. 4. A composition according to claim 3, which comprises at least one oligomeric acrylate ester, preferably a diacrylate ester, of an epoxy resin, and at least one polyurethane acrylate oligomer. 5. A composition according to claim 14, wherein the epoxy ester and the urethane resin are present in a ratio of from about 2:1 to about 3:1 by weight. 6. A composition according to claim 3,14 or 5, wherein both oligomers have terminal acryl or methacryl groups. 7. A composition according to any preceding claim, wherein said polymerisation curing initiator is a photoinitiator. 8. A composition according to claim 7, wherein said photoinitiator is benzoin or an acetophenone such as 2,2-dimethoxy-2-phenylacetophenone or 2,2-diethoxyacetophenone. 9. A composition according to any of claims 1 to 6, wherein said polymersiation curing initiator is a thermal initiator, such as benzoyl peroxide, acetylperoxide or azoisobutyronitrile. 10. The use of a curable adhesive composition, as claimed in any preceding claim, for transferring and mounting a tape-supported histological tissue section onto a specimen support.

Inventors: ORNSTEIN, LEONARD
Applicants: MOUNT SINAI SCHOOL OF MEDICINE OF THE CITY UNIVERSITY OF NEW YORK
Application year: 1983
Main classification: G01N1
IPC codes: G01N1, C09J163, C09J201