FUNGICIDAL HETEROCYCLIC COMPOUNDS

Abstract: A compound of the general formula or a salt or a metal complex thereof, wherein X is oxygen or sulphur; Y represents a group >CR⁴R⁵ or -CR⁴R⁵CR⁶R⁷-; one of R¹ and R² represents an optionally substituted pyrazinyl group, and the other represents an optionally substituted pyrazinyl, pyridyl or phenyl group; each of R³, R⁴, R⁵, R⁶ and R⁷ independently represents a hydrogen atom or an alkyl group; and m is 0, 1 or 2.

Description: Fungicidal Heterocyclic Componds The present invention relates to fungicidal heterocyclic compounds. Accordingly the present invention provides a compound of the general formula: EMI1.1 or a salt or a metal salt camplex thereof, wherein X is oxygen or sulphur; Y represents a group #CR4R5 or -CR4R5CR6R7-; one of R1 and R2 represents an optionally substituted pyrazinyl group, and the other represents an optionally substituted pyrazinyl, pyridyl or phenyl group; each of R3, R4, R5, R6 and R7 independently represents a hydrogen atan or an alkyl group; and m is 0, 1 or 2. Suitable substituents for a pyrazinyl group represented by R1 or R2 may be C(1-4) alkyl, especially methyl, phenyl or cyano groups, and a nitrogen atcm may be substituted by an oxygen ata to form the N-oxide. Preferably, however, the or each pyrazinyl group is unsubstituted. Suitable substituents for a pyridyl group represented by R1 or R2 include halogen atans and C(1-4) alkyl, especially methyl, groups, and the nitrogen atom may be substituted by an oxygen atam to form the N-oxide. Preferably, however, a pyridyl group is unsubstituted. Preferred pyridyl groups are 3-pyridyl groups. A phenyl ring represented by R1 or R2 may be substituted by any of those substituents commonly appearing on aromatic rings; for example the phenyl ring may suitably be substituted by up to 3 substituents each of which may independently be a halogen atom, or a cyano, nitro, alkylsulphonyl or methylenedioxy group, or an optionally substituted phenyl, phenaxy, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, or alkoxycarbonyl group. Preferably an alkyl, alkenyl or alkynyl moiety has up to 4 carbon atoms. When the substituents carried by the phenyl ring are themselves further substituted, these further substituents may suitably be halogen atoms, for example bromine or preferably chlorine or fluorine atoms, cyano, nitro, alkyl, alkoxy, alkylthio, alkylsulphonyl, phenyl, phenoxy or benzyl groups. Preferably each alkyl moiety contains up to 4 carbon atoms. Particularly suitable substituents for a phenyl ring represented by R1 or R2 are bromine, or, preferably, chlorine or fluorine atams, or nitro, cyano, methylsulphonyl, methyl, trifluoro eethyl, Ct1-4)alkoxy, e.g. methoxy, or C(1-4)alkylthio, e.g. methylthio, groups. Preferably the phenyl ring are one or two such substituents; especially preferred are mono- or di-halophenyl groups. Preferably one of R1 and R2 is an optionally substituted pyrazinyl group and the other is an optionally substituted phenyl group Alkyl groups represented by R3, R4, R5, R6 or R7 preferably have up to 4, especially 1 or 2, carbon atoms. Preferably each of R3, R4, R5, R6 and R7 independently represents a methyl group or a hydrogen atom. Preferably at least two of R, R5, R6 and R7 are hydrogen. Preferably X is oxygen. Preferably m is 0. The compounds of formula I are capable of forming acid addition salts. Such salts may be with organic or inorganic acids. For example they may be with sulphuric acids such as toluene or benzene sulphonic acid, with carboxylic acids such as acetic or oxalic acid, or with mineral acids, such as hydrohalic acids, especially hydrochloric acid, or sulphuric or nitric acid. The compounds of formula I also form metal salt complexes. Suitable metals include copper, zinc, manganese and iron, and the anion may be derived from one of the acids listed above. It will be appreciated that the compounds of formula I may contain asymmetric centres depending on the various groups present, for example at the saturated carbon atoms in the thiazolidinone or thiazinone ring, and thus that they may exist in the form of different isomers. Accordingly the present invention encompasses the individual isomers as well as racemic and other mixtures of isomers of these compounds. The invention also provides a process for the preparation of a compound according to the invention, which comprises cyclising a compound of the general formula EMI3.1 in which X, Y, R1, R2 and R3 have the meanings given above, and A represents an -OH or -SH group; and if desired, converting the resulting compound according to the invention into any other compound according to the invention. The reaction is conveniently carried out in a solvent. Suitable solvents include hydrocarbons and chlorinated hydrocarbons, for example a benzenic solvent such as benzene, toluene, xylene or chlorobenzene, or an aliphatic solvent such as tetra-, tri- or di-chioranethane, or 1,2-dichloroethane. If desired, the water generated by the reaction, when Y is a hydroxy group, may be distilled off continuously. In sare cases, the use of a cyclizing or dehydrating agent, for example N,N-dicyclohexylcarbodiimide or N-ethoxycarbonyl1-2-ethoxy-1,2-dihydroquinoline may be desirable, particularly when carrying out the reaction at relatively low temperatures. In general, the reaction may be carried out, for example, at a temperature of from 0 to 1500C. It is usually convenient to carry out the reaction either at room temperature or at the reflux temperature. The derivatives of formula I in which mrl or 2 may be prepared by oxidising the corresponding derivative in which m is less than 1 or 2 respectively. This may be carried out using conventional oxidising agents, for example peroxides such as hydrogen peroxide, or peracids such as metachloroperbenzoic acid or peracetic acid, or potassium permanganate. Conveniently the derivative to be oxidised is dissolved in a suitable solvent, for example a chlorinated hydrocarbon solvent such as chloroform or dichloranethane, or a carboxylic acid such as acetic acid. The derivatives wherein R , R , R or R7 are not hydrogen can if desired be conveniently prepared by alkylation of corresponding hydrogen analogues by methods knawn in the art, such as those described in United Kingdom Patent Application No. 2031 892 A. The thiono derivatives (in which X is sulphur) may, if desired, be prepared from the corresponding derivatives in which X is oxygen by reaction with an inorganic sulphide, for example P2 S5. The reaction may conveniently be carried out in a solvent such as pyridine, and preferably with heating. The compounds of formula I may be prepared in the form of salts or may be converted into salts or complexes by methods knawn in the art, such as reaction with the appropriate acid or metal salt. Thus the basic compound may be dissolved in a suitable solvent, such as an ether, for example diethyl ether, and acid or metal salt added. The compounds of formula I may be isolated from the reaction medium in known manner, and it will be appreciated that different iscmeric forms can be isolated from one another by methods known in the art, for example by chromatography. The compound of formula II may be prepared by reacting an imine of the general formula R2R3C=NR1 (III) with a corrpound of the general formula HS.Y.CX.A. (IV) where R1, R2, R3, X and Y have the meanings given above, and A represents an -OH or -SH group. Preferably the reaction is carried out in the presence of a solvent, for example one of those listed above for the cyclisation reaction, such as toluene, benzene, xylene or chlorobenzene. The reaction temperyture is preferably in the range of form 0 to 150 C, for example at room temperature or at the reflux temperature. In some cases, it may be convenient not to isolate the compound of the general formula II, but to carry out the reaction of the compounds III and IV under conditions such that cyclisation of the resulting compound II takes place in situ. The compounds of formulae III and IV may be prepared by methods known in the art. Thus the imine may be prepared by reacting an amine, R1NH2, with an aldehyde or ketone R2R3C=O, conveniently in the presence of a solvent; the reaction may be carried out at reflux temperature and the water of the reaction distilled off continuously. If desired, the preparation of the imine, and its reaction with a compound of formula IV can be carried out without isolation of the imine, for example by reacting the aldehyde or ketone, R2R3C=O, and amine, R1NH2, with the compound of formula IV, in the presence of a suitable solvent. The compounds of formula I and their salts and metal salt complexes have been found to have fungicidal properties, and accordingly the invention further provides a fungicidal compo- sition comprising a compound according to the invention in association with at least one carrier, and a method of making such a composition which comprises bringing a compound according to the invention into association with at least one carrier. The invention also provides the use of a compound or campo- sition according to the invention as a fungicide. Further in accordance with the invention there is provided a method of combating fungus at a locus, by treating the locus with a ccmpound or composition according to the invention, and particularly a method of protecting crop plants from fungal attack comprising treating crop plants subject to or subjected to fungal attack, seeds of such crop plants or the medium in which such plants are growing or are to be grown with a compound or a composition according to the invention. The compounds according to the invention are generally applied at a dosage rate of, for example, 0.025 to 10kg/ha. In general, for application to plant foliage for prophylactic or therapeutic treatment preferred dosage rates will be in the range 0.1 to 1.0 kg/ha. For soil treatment appropriate rates of application may vary widely, for example 0.5 to 5.0 kg/ha. In use for seed treatment, preferred rates of application may be 1.0 to lOg/kg of seed. A carrier in a composition according to the invention is any material with which the active ingredient is formulated to facilitate application to the locus to be treated, which may for example be a plant, seed or soil, or to facilitate storage, transport or handling. A carrier may be a solid or a liquid, including a material which is normally gaseous but which has been coMpressed to form a liquid, and any of the carriers normally used in formulating fungicidal compositions may be used. A composition according to the invention generally contains from 0.5 to 95% by weight of active ingredient. Suitable solid carriers include natural and synthetic clays and silicates, for example natural silicas such as diatomaceous earths; magnesium silicates, for example talcs; magnesium aluminium silicates, for example attapulgites and vermiculites; aluminium silicates, for example kaolinites, montmorillonites and micas; calcium carbonate; calcium sulphate; synthetic hydrated silicon oxides and synthetic calcium or aluminium silicates; elements, for example carbon and sulphur; natural and synthetic resins, for example coumarone resins, polYvinyl chloride, and styrene polymers and copolymers; solid polychlorophenols; bitu wren; waxes, for example beeswax, paraffin wax, and chlorinated mineral waxes; and solid fertilisers, for example superphosphates. Suitable liquid carriers include water; alcohols, for example isopropanol and glycols; ketones, for example acetone, methyl ethyl ketone, methyl isobutyl ketone and cyclohexanone; ethers; aromatic or araliphatic hydrocyrbons, for example benzene, toluene and xylene; petroleum fractions, for example kerosine and light mineral oils; chlorinated hydrocarbons, for example carbon tetrachloride, perchloroethylene and trichloroethane. Mixtures of different liquids are often suitable. Fungicidal compositions are often formulated and transported in a concentrated form which is subsequently diluted by the user before application. The presence of small amounts of a carrier which is a surface-active agent facilitates this process of dilution. Thus preferably at least one carrier in a composition according to the invention is a surface-active agent. For example, a carposition may contain at least two carriers, at least one of which is a surface-active agent. A surface-active agent may be an emLlsifying agent, a dispersing agent or a wetting agent; it may be nonionic or ionic. Exairples of suitable surface-active agents include the sodium or calcium salts of polyacrylic acids and lignin sulphonic acids; the condensation products of fatty acids or aliphatic amines or amides containing at least 12 carbon atoms in the molecule with ethylene oxide and/or propylene oxide; fatty acid esters of glycerol, sorbitan, sucrose or pentaerythritol; condensates of these with ethylene oxide and/or propylene oxide; condensation products of fatty alcohols or alkyl phenols, for example Er octylphenol or p-octylcresol, with ethylene oxide and/or propylene oxide; sulphates or sulphonates of these condensation products; alkali or alkaline earth metal salts, preferably sodium salts, of sulphuric or sulphonic acid esters containing at least 10 carbon atoms in the molecule, for example sodium lauryl sulphate, sodium secondary alkyl sulphates, sodium salts of sulphonated caster oil, and sodium alkylaryl sulphonates such as sodium dodecyl-benzene suphonate; and polymers of ethylene oxide and copolymers of ethylene oxide and propylene oxide. The carpositions of the invention may for example be formulated as wettable powders, dusts, granules, solutions, emulsifiable concentrates, emulsions, suspension concentrates and aerosols. Wettable powders ususally contain 25, 50 and 75% w of active ingredient and usually contain, in addition to solid inert carrier, 3-10% w of a dispersing agent and, where necessary, 010% w of stabiliser(s) and/or other additives such as penetrants or stickers. Dusts are usually formulated as a dust concentrate having similar composition to that of a wettable powder but without a dispersant, and are diluted in the field with further solid carrier to give a composition usually containing h-10% w of active ingredient. Granules are usually prepared to have a size between 10 and 100 BS mesh (1.676 - 0.152 mm), and may be manufactured by agglarer-ation or impregnation techniques. Generally, granules will contain h-25% w active ingredient and 0-10% of additives such as stabilisers, slow release modifiers and binding agents. Emulsifiable concentrates usually contain, in addition to a solvent and, when necessary, co-solvent, 10-50% w/v active ingredient, 2-20% w/v emulsifiers and 0-20% w/v of other additives such as stabilisers, penetrants and corrosion inhibitors. Suspension concentrates are usually compounded so as to obtain a stable, non-sedimenting flowable product and usually contain 1075% w active ingredient, 0.5-15% w of dispersing agents, 0.1-10% w of suspending agents such as protective colloids and thixotropic agents, 0-10% w of other additives such as defoamers, corrosion inhibitors, stabilisers, penetrants and stickers, and water or an organic liquid in which the active ingredient is substantially insoluble; certain organic solids or inorganic salts may be present dissolved in the formulation to assist in preventing sedimentation or as antifreeze agents for water. Aqueous dispersions and emulsions, for example compositions obtained by diluting a wettable powder or a concentrate according to the invention with water, also lie within the scope of the present invention. The said emulsions may be of the water-in-oil or of the oil-in-water type, and may have a thick 'mayonnaise' like consistency. The compositions may also contain other active ingredients, for example compounds having insecticidal, fungicidal or herbicidal properties. In particular, the use of compositions containing a second fungicidal compound may be advantageous, for example to broaden the spectrum of activity. The invention is further illustrated by the following examples. In these examples the identity of the products was confirmed by NMR analysis. Example 1 Preparation of 2-(2',4'-dichlorcphenyl)-3- pyrazinyl-1 , 3-thiazolidin-4-one A mixture of aminopyrazine (9.5g, 0.1 mole), 2,4-dichlorobenzaldehyde (17.5g, 0.1 mole) and mercaptoacetic acid (9.2g, 0.1 mole) -in toluene (150ml) was stirred and refluxed under a Dean and Stark trap for 16 hours. (2.5my water were collected). The mixture was cooled, and solvent removed in vacuo. The residue was triturated twice with ethanol, and the product, which crystallised on standing, was filtered off and dried. Recrystallization from ethanol gave the 2-(2',4 '-dichlorophenyl) -3-pyrazinyl-1, 3-thiazolidin-4-one in 72% yield as a crystalline solid, mp. 104-50C. Analysis N3C12SOC13H9: Theory 47.85% C 2.76% H 12.88% N Found 48.0% C 2.8% H 13.1% N Example 2 Preparation of 2-(2' ,4'-dichlorophenyl) -3 pyrazinyl-1,3-thiazolidin-4-one-1-oxide. 3-Chloroperbenzoic acid (85%; 5.07g, 0.025 mole) was dissolved in CH2C12 (loom) and the solution cooled in an ice-bath with stirring. 2-(2',4'-Dichlorophenyl)-3-pyrazinyl-1,3-thiazolidin-4-one, prepared as in Example 1, (8.15g, 0.025 mole) was added portionwise, keeping the temperature below 10 C. The mixture was stirred for a further 15 minutes at 5 5 C , and then for 2 hours at ambient temperature. After filtration, the filtrate was washed twice with saturated aqueous NaHCO3 solution, and then twice with water and dried (MgSO4). After removal of solvent in vacua, the residue was dissolved in diethyl ether (500ml) and the solution poured into approximately 1 litre of 40/60 petroleum spirit. The 2-(2',4'-dichlorophenyl)-3-pyrazinyl-1,3-thiazolidin4-one-l-oxide which separated was filtered off, washed with 40/60 petroleum spirit and dried. Yield 91%, mp. 98-100 C. Analysis N3C12S02C13Hg: Theory 45.61% C 2.63% H 12.28% N Found 45.8% C 2.88 H 11.9% N Example 3 Preparation of 2-(2',4'-dichlorophenyl)-3- pyrazinil-1,3-thiazolidin-4-one-1,1-dioxide To a stirred solution of 2-(2',4'-dichlorophenyl)-3-py- razinyl-l,3-thiazolidin-4-one (8.15g, 0.025 mole) prepared as in Example 1 in glacial acetic acid (75ml) at 15-200C, a solution of powdered potassium permanganate (11.85g, 0.075 mole) in water (250ml) was added dropwise during half an hour, and stirring was continued at 20 C for 2.5.hours, by which time the purple colour of the permanganate was discharged. This solution was added dropwise to a stirred solution of sodium metabisulphite (50g) in water (500ml). The tan-coloured solid that separated was filtered off, washed with water (3 x 250ml) and dried. Recrystallization from ethanol gave the 2-(2',4'-dichlorophenyl)-3-py razinyl-1,3-thiyzolidin-4-one-1,1-dioxide in 26% yield, mp. 164-60C. Analysis N3C12SO3C13H9: Theory 43.58% C 2.51% H 11.73% N Found 43.6% C 2.4% H 11.? Example 4 2-(4'-chlorophenyl-3-pyrazinyl-1,3-thiyzolidin 4-one This compound was prepared by a method analogous to that of Example 1. Melting Point: 126-128 C. Analysis N3OSC1C13H10 Theory 53.52% C 3.43% H 14.41% N Found 53.7% C 3.5% H 14.4% N Example 5 Copper II chloride complex of the compound of Example 4. The compound of Example 4 (2.92g, 0.01 ir1) was dissolved in absolute ethanol (35 ml) at 60 0C. A solution of anhydrous copper II chloride (0.673g, 0.005 mol) in absolute ethanol (lOml) was added. After stirring for h hour, the resultant blue solid was filtered off, washed with ethanol and then with diethyl ether, and dried. The complex of 2 moles of CuC12 per mole of thiazolidinone was obtained in 97% yield, melting point 194 C. Analysis N6O2S2Cl4CUC26H20 Theory 43.48% C 2.79% H 11.71% N Found 43.4% C 2.9% H 11.4% N Example 6 2-(4'-chlorophenyl)-3-pyrazinyl-1,3-thiyzolidin 4-thione Phosphorus pentasulphide (6.66g, 0.003 mol) was added to a stirred solution of the compound of Example 4 (8.75g, 0.03 mol) in dry dioxan under a nitrogen atmosphere. The mixture was heated at 110-120 C for 8 hours. After cooling, the solution was decanted and the solvent removed in vacuo. The residue was purified by chromatography on silica gel, eluting with diethyl ether/hexane (2/1), and then recrystallised from cyclohexane to give the desired product in 29% yield, melting point 118-1200C. Example 7 2-(4'-chlorophenyl)-tetrahydro-3-pyrazinyl-4H-1,3 thiazin-4-one A mixture of aminopyrazine (19g, 0.2 mol) and 4chloro benzaldehyde (28.lg, 0.3 mol) in toluene (250ml) was stirred and refluxed under a Dean and Stark trap for 16 hours. After cooling, 3-mercaptopropionic acid (23.3g, 0.22 mol) dissolved in a little toluene was added. The solid resulting was filtered off, washed with toluene and then with petroleum spirit, and dried. lOg of this solid was dissolved in methylene chloride (250ml), N,N'dicyclohexylcarbodiimide (log) was added, and the mixture was stirred at roam temperature for 12 days. The mixture was then filtered, and the solvent removed from the filtrate in vacuo. The residue was purified by chromatography over silica gel, eluting with diethyl ether, and then by further chromatography over silica gel, eluting with 10% diethyl ether in methylene chloride. The desired compound was obtained in 20% yield, melting point 112.5-114.5 C. Analysis N3QSC1C14H12 Theory 54.99% C 3.93% H 13.75% N Found 55.2% C 3.98 H 13.6% N Examples 8 to 20 The following campounds were prepared according to methods similar to those of the above examples. The melting points and chemical analysis of the compounds are given in Table I below. Table I EMI13.1 Example <SEP> In <SEP> the <SEP> General <SEP> FormulaI <SEP> Melting <SEP> Analysis <tb> No. <SEP> Point <SEP> C <SEP> Theory <SEP> Found <tb> <SEP> R1 <SEP> R2 <SEP> R3 <SEP> Y <SEP> m <SEP> X <SEP> C <SEP> H <SEP> N <SEP> C <SEP> H <SEP> N <tb> 8 <SEP> 2,4-dichlorophenyl <SEP> pyrazinyl <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 137-138.5 <SEP> 47.85 <SEP> 2.76 <SEP> 12.88 <SEP> 47.9 <SEP> 2.8 <SEP> 12.8 <tb> 9 <SEP> 2,4-dichlorophenyl <SEP> pyrazinyl <SEP> H <SEP> CH2 <SEP> 2 <SEP> 0 <SEP> 214-222 <SEP> 43.58 <SEP> 2.51 <SEP> 11.73 <SEP> 44.0 <SEP> 2.5 <SEP> 11.8 <tb> <SEP> (decomp.) <tb> 10 <SEP> pyrazinyl <SEP> 4-chloro- <SEP> H <SEP> CH.CH3 <SEP> 0 <SEP> 0 <SEP> 91-92 <SEP> 54.99 <SEP> 3.93 <SEP> 13.75 <SEP> 55.1 <SEP> 4.0 <SEP> 13.8 <tb> <SEP> phenyl <SEP> (isomer <SEP> A) <tb> 11 <SEP> pyrazinyl <SEP> 4-chloro- <SEP> H <SEP> CH.CH3 <SEP> 0 <SEP> 0 <SEP> 143-146 <SEP> 54.99 <SEP> 3.93 <SEP> 13.75 <SEP> 55.1 <SEP> 3.9 <SEP> 13.8 <tb> <SEP> phenyl <SEP> (isomer <SEP> B) <tb> 12 <SEP> pyrazinyl <SEP> 4-chloro- <SEP> H <SEP> C(CH3)2 <SEP> 0 <SEP> 0 <SEP> 134-135.5 <SEP> 56.34 <SEP> 4.39 <SEP> 13.15 <SEP> 56.6 <SEP> 4.6 <SEP> 13.2 <tb> <SEP> phenyl <tb> 13 <SEP> pyrazinyl <SEP> 4-flouro- <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 124-126 <SEP> 56.73 <SEP> 3.64 <SEP> 15.27 <SEP> 57.5 <SEP> 3.9 <SEP> 15.3 <tb> <SEP> phenyl <tb> 14 <SEP> pyrazinyl <SEP> 4-chloro- <SEP> CH3 <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 148-150 <SEP> 54.99 <SEP> 3.93 <SEP> 13.75 <SEP> 55.3 <SEP> 4.0 <SEP> 13.8 <tb> <SEP> phenyl <tb> Table I continued ... EMI14.1 Example <SEP> In <SEP> the <SEP> Genral <SEP> Formula <SEP> I <SEP> Melting <SEP> Analysis <tb> No. <SEP> Point C <SEP> Theory <SEP> Found <tb> <SEP> R1 <SEP> R2 <SEP> R3 <SEP> Y <SEP> m <SEP> X <SEP> C <SEP> H <SEP> N <SEP> C <SEP> H <SEP> N <tb> 15 <SEP> pyrazinyl <SEP> phenyl <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 129-131 <SEP> 60.70 <SEP> 4.28 <SEP> 16.34 <SEP> 60.9 <SEP> 4.4 <SEP> 16.3 <tb> 16 <SEP> pyrazinyl <SEP> 4-methyl- <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 136-138 <SEP> 61.99 <SEP> 4.80 <SEP> 15.50 <SEP> 62.1 <SEP> 4.8 <SEP> 15.3 <tb> <SEP> phenyl <tb> 17 <SEP> pyrazinyl <SEP> 4-bromo- <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 140-142 <SEP> 46.43 <SEP> 2.98 <SEP> 12.50 <SEP> 46.4 <SEP> 2.9 <SEP> 12.4 <tb> <SEP> phenyl <tb> 18 <SEP> pyrazinyl <SEP> 3,4-di- <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 122-125 <SEP> 47.85 <SEP> 2.76 <SEP> 12.88 <SEP> 47.5 <SEP> 2.8 <SEP> 12.8 <tb> <SEP> chloro <SEP> phenyl <tb> 19 <SEP> pyrazinyl <SEP> 3-pyridyl <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 154-156 <SEP> 55.81 <SEP> 3.88 <SEP> 21.71 <SEP> 55.9 <SEP> 3.9 <SEP> 21.7 <tb> 20 <SEP> pyrazinyl <SEP> 4-chloro- <SEP> H <SEP> C(CH3)2CH2 <SEP> 0 <SEP> 0 <SEP> 119-120 <SEP> 57.57 <SEP> 4.80 <SEP> 12.59 <SEP> 58.3 <SEP> 4.8 <SEP> 12.8 <tb> <SEP> phenyl <SEP> (CH2 <SEP> adjacent <tb> <SEP> the <SEP> B <SEP> atom) <tb> Table I continued ... EMI15.1 Example <SEP> In <SEP> the <SEP> Genral <SEP> Formula <SEP> I <SEP> Melting <SEP> Analysis <tb> No. <SEP> Theory <SEP> Found <tb> <SEP> R1 <SEP> R2 <SEP> R3 <SEP> Y <SEP> m <SEP> X <SEP> Point <SEP> C <SEP> C <SEP> H <SEP> N <SEP> C <SEP> H <SEP> N <tb> 21* <SEP> pyrazinyl <SEP> 4-chloro- <SEP> H <SEP> CH2 <SEP> 0 <SEP> 0 <SEP> 148-151 <SEP> 47.56 <SEP> 3.35 <SEP> 12.80 <SEP> 47.6 <SEP> 3.3 <SEP> 12.7 <tb> <SEP> phenyl <tb> 22 <SEP> pyrazinyl <SEP> 2,4-di <SEP> H <SEP> CH2CH2 <SEP> 0 <SEP> 0 <SEP> 153-156 <SEP> 49.41 <SEP> 3.24 <SEP> 12.35 <SEP> 49.7 <SEP> 3.5 <SEP> 12.4 <tb> <SEP> chloro <SEP> phenyl <tb> 23 <SEP> pyrazinyl <SEP> 2,4-di <SEP> H <SEP> C(CH3)2CH2 <SEP> 0 <SEP> 0 <SEP> 213-217 <SEP> 52.17 <SEP> 4.08 <SEP> 11.41 <SEP> 51.3 <SEP> 4.1 <SEP> 11.3 <tb> <SEP> chloro- <SEP> (CH2 <SEP> adjacent <tb> <SEP> phenyl <SEP> the <SEP> S <SEP> atom) <tb> 24 <SEP> pyrazinyl <SEP> 2,4-di- <SEP> H <SEP> C(CH3)2CH2 <SEP> 0 <SEP> 0 <SEP> 176-178 <SEP> 47.19 <SEP> 3.09 <SEP> 11.80 <SEP> 47.3 <SEP> 3.0 <SEP> 11.8 <tb> <SEP> chloro- <SEP> (CH2 <SEP> adjacent <tb> <SEP> phenyl <SEP> the <SEP> S <SEP> atom) <tb> 25 <SEP> pyrazinyl <SEP> 2,4-di- <SEP> H <SEP> C(CH3)2CH2 <SEP> 0 <SEP> 0 <SEP> 179-181 <SEP> 45.16 <SEP> 2.96 <SEP> 11.29 <SEP> 45.3 <SEP> 3.0 <SEP> 11.3 <tb> <SEP> chloro <SEP> (CH2 <SEP> adjacent <tb> <SEP> phenyl <SEP> the <SEP> S <SEP> atom) <tb> 26* <SEP> pyrazinyl <SEP> 2,4-di- <SEP> H <SEP> C(CH3)2CH2 <SEP> 0 <SEP> 0 <SEP> 154-156 <SEP> 44.62 <SEP> 3.19 <SEP> 11.16 <SEP> 44.7 <SEP> 3.1 <SEP> 10.9 <tb> <SEP> chloro- <SEP> (CH2 <SEP> adjacent <tb> <SEP> phenyl <SEP> the <SEP> S <SEP> atom) <tb> Example 24 The activity of campounds according to the invention was determined by the following test procedures. In all cases, the test solution was obtained by diluting with water to give the required dosage, a stock solution containing the active material in acetone (50%), surfactant (0.4%) and water. (a) Activity against apple powdery mildew (Podosphaera leuco tricha; P.1) This test is a direct anti-sporulant one using a foliar spray. The upper surfaces of leaves of whole apple seedlings were inoculated by spraying with an aqueous suspension containing 105 conidia/ml 2 days prior to treatment with the test compound. The inoculated plants were immediately dried and kept at glasshouse ambient temperatures and humidity prior to treatment. The plants were sprayed at a dosage of 1 kilogram of active material per hectare using a track sprayer. After drying the plants were returned to a compartment at ambient temperature and humidity for up to 9 days, followed by assessment. Assessment was based on the percentage of the leaf area covered by sporulation carpared with that on leaves of control plants. (b) Activity against potato/tanato late blight (Phytophthora infestans; P.i.p.) The test measures the direct protectant activity of compounds applied as a foliar spray. Tomato plants, Cultivar Ailsa Craig, 1-15 cms high, were used. The whole plant was sprayed at a dosage of 1 kilogram of active material per hectare using a track sprayer. The plant was then inoculated up to 6 hours after treatment with the test compound, by spraying with an aqueous suspension containing 5 x 103 zoospores/ml. The inoculated plants were kept in high humidity for 3 days Assessment was based on comparison between the levels of disease on the treated and control plants. (c) Activity against barley powdery mildew (Erysiphe graminis; Eq.) The test measures the direct anti-sporulant activity of compounds applied as a foliar spray. For each campound about 40 barley seedlings were grown to the one-leaf stage in a plastic pot of sterile potting compost. Inoculation was effected by dusting the leaves with conidia of Erysiphe graminis, spp. hordei. 24 hours after inoculation the seedlings were sprayed at a dosage equivalent to 1 kg of active material per hectare. First assessment of disease was made 5 days after treatment, when the overall level of sporulation on the treated plants were cc8npared with that on control plants. (d) Activity against barley powdery mildew (Erysiphe gramanis; Eg (soil)) The test measures the systemic protectant activity of pounds applied as a soil drench. For each compound, soil was drenched at a dosage equivalent to 10kg/ha. About 25 barley seeds, cv. Golden Promise, were placed on top of the treated soil, and covered with about 1cm untreated soil. After about 7 days when the seeds had germinated, spores of the fungus were dusted onto the plants. After 5 days, the level of sporulation was compared with that on control plants. (e) Activity against vine grey mould (Botrytis cinerea; B.c) The test is a direct eradicant one using a foliar spray. The under-surface of detached vine leaves were inoculated by pipetting ten large drops of an aqeuous suspension containing 5 x 105 conidia/ml on to them. The inoculated leaves were kept uncovered overnight during which time the fungus penetrated the leaf and a visible necrotic lesion became apparent where the drop was made. The infected regions were sprayed directly with a dosage of 1 kg of active material per hectare using a track sprayer. When the spray had dried the leaves were covered with petri dish lids and the disease allowed to develop under the moist conditions. The extent of the necrotic lesion beyond the original drop together with the dosage of sporulation was compared with that on control leaves. (f) Activity against peanut leaf spot (Cercospora arachidicola; C.a) The test is a direct eradicant one using a foliar spray. The upper surfaces of the leaves of peanut plants (12-20cms high, in monopots were inoculated by spraying with an aqueous suspension containing 105 conidia/ml 40-43 hours prior to treatment with the test carpound. The inoculated plants were kept at high humidity and then allcwed to dry during the interval between innoculation and treatment by spraying at a dosage of 1kg of active material per hectare using a track sprayer. After spraying the plants were moved to a humid catpart:inent at 25-28 & for a further period of up to 10 days. Assessment was based on a comparison between the levels of disease on the treated and control plants. The extent of disease control achieved in these tests is expressed as a control rating in which disease control greater than 80% is given the rating 2, and control of between 50 and 80% is given the rating 1. The results are given in Table II below. Table II Test Results EMI19.1 Compound <SEP> Test <SEP> Result <tb> of <SEP> Example <SEP> P.i.p. <SEP> Eg <SEP> Eg(soil) <SEP> P.1. <SEP> B.c. <SEP> C.a. <tb> No. <tb> 1 <SEP> 2 <SEP> 1 <SEP> 2 <tb> 2 <SEP> 1 <tb> 3 <SEP> 1 <SEP> 1 <tb> 4 <SEP> 2 <SEP> 1 <tb> 5 <SEP> 2 <tb> 6 <SEP> 2 <tb> 7 <SEP> 2 <SEP> 2 <SEP> 2 <SEP> 2 <tb> 8 <SEP> 2 <SEP> 2 <tb> 9 <SEP> 1 <SEP> 2 <tb> 10 <SEP> 2 <SEP> 1 <SEP> 1 <tb> 11 <SEP> 2 <SEP> 1 <tb> 12 <SEP> 2 <SEP> 2 <tb> Table II Test Results CTD. EMI20.1 Compound <SEP> Test <SEP> Result <tb> of <SEP> Example <SEP> P.i.p. <SEP> Eg <SEP> Eg(soil) <SEP> P.1. <SEP> B.c. <SEP> C.a. <tb> No. <tb> 13 <SEP> 2 <SEP> 2 <SEP> 2 <tb> 14 <SEP> 2 <tb> 15 <SEP> 1 <tb> 16 <SEP> 1 <tb> 17 <SEP> 1 <SEP> 1 <tb> 18 <SEP> 1 <tb> 19 <SEP> 1 <tb> 20 <SEP> 2 <SEP> 2 <tb> 21 <SEP> 2 <SEP> 2 <SEP> 1 <tb> 22 <SEP> 1 <SEP> 2 <SEP> 1 <SEP> 2 <SEP> 2 <tb> 23 <SEP> 2 <SEP> 2 <SEP> 2 <SEP> 2 <tb>

Claims: CLAIMS 1. A compound of the general formula EMI21.1 or a salt or a metal complex thereof, wherein X is oxygen or sulphur; Y represents a group CR4R5 or -CR4R5CR6R7 one of R1 and R2 represents an optionally substituted pyrazinyl group, and the other represents an optionally substituted pyra zinyl, pyridyl or phenyl group; each of R31 R4, R51 R6 and R7 independently represents a hydrogen atan or an alkyl group; and m is 0, 1 or 2. 2. A compound as claimed in claim 1, in which any pyrazinyl group represented by R1 or R2 is unsubstituted or substituted by one or more C(1-4) alkyl, phenyl or cyano groups; any pyridyl group represented by R1 or R2 is unsubstituted or substituted by one or more halogen atoms or C(1-4) alkyl groups; and any phenyl group represented by R1 or R2 is unsubstituted or substituted by one or more halogen atoms and/or nitro, cyano, methylsulphonyl, methyl, trifluorarethyl, C(1-4)alkoxy or C(l-4)alkylthio groups. 3. A compound as claimed in claim 2, in which any pyrazinyl or pyridyl group represented by R1 or R2 is unsubstituted, and any phenyl group represented by R1 or R2 is substituted by one or two halogen atoms. 4. A compound as claimed in any one of claims 1 to 3 in which one of R1 and R2 represents an optionally substituted pyrazinyl group and the other represents an optionally substituted phenyl group. 5. A compound as claimed in any one of claims 1 to 4, in which each of R3, R4, R5, R6 and R7 independently represents a methyl group or a hydrogen atan. 6. A campound as claimed in claim 5, in which at least two of R4, R5, R6 and R7 represent hydrogen atoms. 7. A compound as claimed in any one of claims 1 to 6, in which X represents an oxygen atom. 8. A carpound as claimed in any one of claims 1 to 7, in which m is 0. 9. A compound as claimed in claim 1, and named in any one of Fxamples 1 to 23 herein. 10. A process for the preparation of a compound as claimed in claim 1, which comprises cyclising a compound of the general formula EMI22.1 in which R1, R2, R, X and Y have the iteanings given in claim 1, and A represents an -OH or -SH group; and if desired, converting the resulting canpound according to the invention into any other compound according to the invention. 11. A process as claimed in claim 10, in which the compound of the general formula II has been prepared by the reaction of a compound of the general formula R2R3C = NR1 (III) with a compound of the general formula HS.Y.CX.A. (IV) where R1, R2, R3, X, Y and A have the meanings given in claim 10. 12. A process as claimed in claim 10, carried out substantially as described in any one of Examples 1 to 7 herein. 13. A compound as claimed in claim 1, whenever prepared by a process as claimed in any one of claims 10 to 12. 14. A fungicidal composition which camprises a campound as claimed in any one of claims 1 to 9 and 13, together with a carrier. 15. A composition as claimed in claim 14, which comprises at least two carriers, at least one of which is a surface active agent. 16. A method of combating fungal growth at a locus, which comprises treating the locus with a compound as claimed in any one of claims 1 to 9 and 13 or a composition as claimed in either claim 14 or claim 15.

Inventors: TEN HAKEN, PIETER, WEBB, SHIRLEY BEATRICE
Applicants: SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.
Application year: 1983
Main classification: C07D417
IPC codes: A01N43, C07D417, C07F1