DETECTION OF MALIGNANT TUMOR CELLS

Abstract: Production of two products which are distinct species of anti-malignin antibody, and the production of three artificially produced species of cell each of which has the distinguishing characteristic of manufacturing either one or both species of anti-malignin antibody. These anti-malignin products are useful to detect the presence of cancerous or malignant tumor cells. These anti-malignin products attach preferentially to cancerous or malignin tumor cells in cell collections in vitro or in vivo and thus can be detected by any attached visible or other signal emitter. This preferential attachment to malignant tumor cells also makes these products useful for metabolic and therapeutic purposes.

Description: Λ Title: Detection of Malignant Tumor Cells ^ This invention relates to (1) the production of two products which ' are distinct species of anti- malignin antibody; and (2) the production of three arti icially-produced species of cell, each of which has the distinguishin characteristic of manufact¬ uring either one or both species of anti-malignin antibody; whereby the above products, both the anti¬ bodies themselves and the cells which produce them, are useful for diagnostic, metabolic and therapeutic purposes. The process of fusion of cell hybrids, is now a routinely used and accepted procedure in the art (Monoclonal Antibodies, Cesar Milstein, Scientific American, May 1980, pp. 66-74*). The production of antibodies by the injection of tumor cells into animals has also been a corrmon procedure in th'e art for many years. An U.S. patent (No. ,172,124, Hillary Koprowski and Carlo M. Croce) relates to a method of producing antibodies to whole tumor cells, the critical first step of which is the injection into-an animal of whole cells from various tumors, and therefore dif.fers . from the present invention which does not util ize the injection of whole cells but rather requires the use of a specific polypeptide composition, Malignin, the subject of my U.S. Patents No. 4, 195,017 and 4,196,186, to produce specific species of a specific antibody, anti- mal ignin antbody. Malignin was earlier used to produce anti-malignin antibody, also a subject of my earlier patents above. But whereas these patents describe the production of polycl ' onal anti-malignin antibody in marr-mals, the anti- malignin antibodies of the present invention are mono¬ clonal, and are the products of single artificially produced cells. In addition to a different mode of production, as will be set forth herein, the present monoclonal antibodies have unique properties, and should therefore be uniquely referred to in order to distinguis them from the anti-malignin antibody which is polyclonai is produced in marrrnals, and has different properties. The artificially produced cell lines themselves, which have been produced by the present invention, and have the ability to produce Monoclonal Anti-Mai ignin Antibodies, have themselves the characteristic novel feature that a single (monoclonal) line or type of cell has been produced artificially with the patented product Malignin, and perpetuated _rι _____ _ with the ability to produce Monoclonal Anti -Mai ignin Antibody. Further, thi novel cell line can produce Monoclonal Anti-Mai ignin Antibody in perpetuity and in any quantities desired. These new artificial ceils are therefore herewith design ated Monoclonal Anti-Mai ignin Antibody-Producing Cells. The new cells have iπmediate uses which are related to the uses of their product antibody, i.e. both diagnostic and therapeutic uses. Thus, the earlier patents referre to above make clear both in their specifications and their examples the use of the .antibody diagnost ical ly to identify the presence of either the antigen Malignin; or any cells which contain Malignin, or to treat therapeut- ically (i.e. destroy) such cells, i.e. malignant or cancerous cells, by the specific reaction of anti-maligni antibody with its specific antigen malignin, whether in solution or fixed in cells (see Examples 11, 11A, 12 for the use of the antibody to stain cancer cells specificall in immunof 1uoresence, and see Example 13 for the use of the antibody to identify or attach specifically to cance cells carrying either a s igna1 -emi t ter for identificatio and localization of the cancer cells in the body, or carrying an anti-cancer drug or chemical to be concent¬ rated in the cancer cell for its destruction, as well as Examples 16, 17 where the antibody alone is used to treat (destroy) cancer cells). From the earliest production by the inventor of anti-malignin antibody (issued U.S. Patents Nos. 4,195,017 and 4,196,186) two const i tuent- species of the antibody were recognized: (1) Fast Target-attaching- globulin (F-TAG), which combined rapidly _n ______ within ten minutes, with its specific inrmobilized antigen malignin; and-(2) Slow Target-attaching-g1obu1 in (S-TAG), which combined slowly _uι vj_tr_o, within two hours, with its specific inrmobilized antigen malignin (see Examples 10,10A). Both S-TAG and F-TAG were produced from blood serum, and the determination of their concentration in the serum of individuals became the basis of the cancer diagnostic test which is the subject of issued Patent No. 4,196,186. The method of production of each species however never yielded either one completely free of the other. The present invention is a marked improvement since it describes the production of a'n unique novel cell line which produces only the S-TAG species, one cell ine which produces only the F-TAG species, and* one ce i l line which produces both species. As summarized above, the ability of the previous polyclonai anti-mali nin antibody, which contained both species, to destroy cancer cells specifically (cytotox- icity) was described in U.S. Patent No. 4,195,017. I have now found that the single species of monoclonal antibody product produced here for the first time for S-TAG, Monoclonal Ant i -Ma1 igni n Antibody - Slow (MAMA-S), attaches preferentially to cancer cells but does n-ot destroy them. Also, the single species of monoc1ona1 antibody product here produced for the first time for O F-TAG, Monoclonal Ant i-Mai ignin Antibody - Fast (MAMA-F) attaches preferentially to cancer cells but does not destroy them. The species of combined antibody here produced for the first time by monoclonal producer cells, designated MAMA-FS, as well as an artificial mixture of the two antibodies MAMA-S and MAMA-F, attach preferent¬ ially to cancer cells and destroy them. The separation of the attachment from the destruction functions of the species of the anti-malignin antibody described in this invention has important applications in the separate uses of the antibody now possible for diagnosis on the one hand and for treatment (destruction of cancer cells) on the other. The respective novel producer cells are designated MAMA-S Producer, MAMA-F Producer, and MAMA-FS Producer, each being uniquely characterized by its specific antibody product. The careful study over the past seven years of the possible relation of the concentration of anti-malignin antibody in the serum of individual patients suffering from cancer has provided unequivocal data presented in this, application (Example 10A) that patients who survive longer, 13 to 6 months, have higher levels of antibody than those who died in one year. Of all patients with low levels of antibody (83.3%) were dead within one year (mean .4 + 3.5 mos.). This clear association of survival- with increased amounts of anti-malignin antibody gives new significance to the therapeutic usefulness of this antibody. Whereas the therapeutic use of anti-malignin antibody was clearly demonstrated in the earlier patents of this series (cytotoxici ty to cancer cells), it was not known how much difference the availability of extra anti¬ body would make to the balance between cancer and normal cells, that is, how important this therapy might be to the survival of the cancer patient. The present cflinicai study makes it clear that the level of anti-malignin antibody available appears- to be clearly associated with survival . *%QZE The products described in this application whereby the patented product malignin has been used to produce novel cells which themselves manufacture in perpetuity and in virtually limitless quantities specific preferentially-attaching and cancer eel 1 -destroying anti-malignin antibodies therefore acquire added signif¬ icance as novel therapeutic anti-cancer products. These novel cells, which have been produced artif¬ icially only through contact with the patented product malignin, carry the permanent instruction in their genetic apparatus to manufacture the particular antibody product indefinitely. They also carry the * instruction to continue to divide indefinitely- Both these instruct ions are seen to be carried out in the Examples herein. It follows to those familiar with the art that the particular cellular constituents which carry this geneti information can now be isolated and induced to perform their particular functions in the antibody manufacture i-2 ∑il SL should this be particularly useful. For exampl should there be an efficiency, cost or other advantage in doing so, the nucleic acid of the producer cell which carries the specific information for manufacturing the particular monoclonal anti-malignin antibody produced by that particular cell can now be removed and isolated fro the other cellular constituents and inserted into another type of cell, for example, bacterial, which may, e.g., divide more quickly, be less susceptible to contamination during bulk manufacture, or be less costly to maintain in the laboratory continuously-. This is only one example of the application of any method practiced by the art which may only be used once the specific cell, MAMA-S, MAMA-F or MAMA-FS, produced by the specific product: malignin, has been produced and shown to be sel -perpetuat ing as described below. - Anti-malignin antibody reacts specifically immuno- logically not only with the antigen malignin, but also with structurally closely related products such as Astrocytin, Recognin L and Recognin M. The present invention therefore continues to be directed to the novel group of compounds, herein termed Recognins. Recognins are made by treating tumor cells or artificial cancer cells and separately the desired products. The Recognins may be used to prepare their Chemoreciprocals , i.e., by contacting the Recognins, or the Recognins on a support, with body fluids. These Chemoreciprocals are useful for diagnostic and therapeutic purposes, i.e., for diagnosing and treating cancers. One of the Recognins of the present invention is -Astrocyct in. -Astrocytin has been produced from brain tumor tissue, preferably brain glioma tumor tissue. Protein fractions containing the Astrocytin precursor are first extracted from the tissue. A preferred method of accomplishing the extraction is to treat, the tissue with a neutral buffer under conditions of homogenizat ion, or other techniques, to disrupt the cells and tissues in order to solubilize protein fractions which contain the Astrocytin precursor. At this point, the Astrocytin precursor is still bound to many large molecular weight substances, includin proteins, g1 ycoproteins , 1 ipoproteins , nucleic acids and nucleo-proteins , for example. The solubilized proteins are then separated from the resulting tissue extract. The extract solution from the tissue is clarified to remove insoluble particles. The low molecular weight contaminants are removed from the resulting solution, by a perevaporat ion concentration technique. The solution which is obtained is then treated to cleave Astrocytin precursor from other contaminants in order to obtain the protein fraction having a pK range between 1 and 4-. Thus for example, the solution is placed on a chromatographic column and eluted with increasingly acidic solvents. All of the fractions which are eluted in the neutral or acid O range down to pK 4 are discarded and those fractions with pK range 1 - 4 are collected. The eluate is treated to yield a product having a molecular weight of 8,000. This is accomplished, for example, by first filtering the material to remove 1ow-molecul ar-weight substances, i.e., those below 1 - 000 molecular weight, and filtering again .to remove those above 25 , 000 . The fraction having a molecular weight between 1,000 and 25,000 is ' then further treated, i.e., by thin layer gel (TLG) chromatography, to y iel d.Astrocyt i n. Thus, Astrocytin may be produced by extracting brain glioma tumor tissue with a neutral buffer, by rep¬ eated homogeni ' zat ion and by high speed centr i fuging, separating from the resulting extract the fraction having a pK range of from 1 to 4, separating from said fraction the substances having a high molecular weight, i.e., up to 230,000, and isolating therefrom the product Astrocytin having a molecular weight of 8,000. The product Astrocytin prepared in accordance with this process is characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, having a spectrophotometr ic absorption peak wave length of 280 mμ . and having a molecular weight of 8,000. Astrocytin is also characterized by having a very high percentage of residues of glutamic acid' and aspartic acid and a very high ratio of these acids to histidine.. A further analysis of Astrocytin is provided below. In a manner similar to that described above, another Recognin., called Malignin, has been produced from art¬ ificial cancer cells, i.e., cancer cells grown J_rι y__Ttr_o. Malignin has a molecular weight of 10,000 and a sfmilar but distinct aminoacid residue composition to Astro¬ cytin, i.e., high amounts of glutaminic acid and aspartic acid and high ratios of these acids to histidine. A further analysis of Malignin .is provided below. Thus, Malignin can be produced by extracting art¬ ificial cancer cells grown in culture with a neutral buffer by repeated homogenization and high speed cent- rifuging, separating from the resulting extract the fraction having a pK range of I to 4, separating from said fraction the substances having a high molecular weight, i.e. up to 230,000 and isolating therefrom the product having a molecular weight of 10,000. Malignin prepared in accordance with this process is characterized by forming a single line precipitate with its speci-fic antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, having a spectro- photometric absorption peak wave length of 280 mμ and having a molecular weight of 10,000. Recognins are further characterized by being capable of complexing with bromoacetylcel 1ulose to form bromo- acety1 -eel lulose-Recognin and producing specific Anti- Recognin antibodies upon injection into maπmals, the Anti -Recognins being toxic to brain tumor cells J_n yit ' ro and producing fluorescence of glioma cells when coupled with fluorescein, as described in further detail below. Recognins, such as Astrocytin, Malignin and similar substances are useful as products which may be intro- ~ duced into a biological s-ystem to reduce foreign reaction such as by coating a material with a Recognin. A further example may be to introduce a Recognin in order to produce the Chemoreciprocals in the biological system. They may also be used nutritionally to encourage the growth of a particular biological system of which they are a part. A further use for Recognins is the production of Target reagents which comprise the complexes of the Recognins .with carriers to facilitate their applicability in biol- ogical systems. Thus, for example, the complex convey.s the phys icαchemlcal characteristics of the Recognin itself. The carrier should be selected from those which form a complex with the Recognin and which 5 are substantially biologically inert. Any substance known in the art which will form a stable complex with polypeptides or proteins may be •useful for complexing with Recognins. An example is a cellulose-based material, such as bromoacetyi- 1 0 cellulose. In addition to being inert to the biolog¬ ical system, the carrier should not alter the specific physicochemi cal properties of the Recognin which are useful for the purposes set forth herein. The complex of the Recognin and its carrier is 15 useful for producing, separating and identifying its chemoreciprocal in any biological system with which it is brought into contact. The Recognin-carr ier complex is also useful for stimulating the production of its chemoreciprocal precursor in any biological system into 20 which it is introduced. One class of Chemoreciprocal s are the ant i -Recognin e.g., ant i-Astrocytin and ant i-Mal ignin. These may be made by injecting the Recognin into a biological system. An irrmunologi cal 1y effective dose of Recognin is brought 25 into contact with bodily tissues or fluids in a manner which induces an antibody response in accordance with techniques known in the art for producing antibodies. .The ant i-Recognins may be used for the delivery o.f materials such as diagnostic, nutritional and therap- 30 eutic agents to specific cells or sites in a biological * system which comprises introducing said agent in complexed form with the anti-Recognin into the biolog¬ ical system. The ant i -Recognins are also useful for diagnosing the presence of tumor cells in an hist-ology 35 section, by applying the Ant i -Recogn i n conjugated with a labelling substance such as a dye or radio-active substance, " to said section, whereby staining or radio¬ active labelling occurs only with 'tumor cells. Yet another use for ant i-Recogήins is for increasing the yield of other useful Chemoreciprocal products (such as TAG, described below) from a marrmal which comprises injecting an irrmunological ly effective dose of the Recognin into the manrmal, or other biological system. Another class of Chemoreciprocals is Target reagents compiexed with their chemoreciprocal s . For example, the Target product of Astrocytin compiexed with a carrier such as bromoacety1eel lulose is brought into contact with anti-Astrocytin . This type of compound may be compiexed with, and used for, the delivery of diagnostic, nutritional and therapeutic agents to specific cells or sites in a biological system. These compounds may also be used for purification procedures. For example, Anti -Astrocyt in may be made by the de- complexing of Bromoacety1ce1 lulose-Astrocyt in-Anti- Astrocytin by hydrolytic treatment with an acid or proteinase enzyme. Target reagents are also useful for increasing the amount of TAG products (described below) in a biological system, such as by bringing an irrmuno- logically effective dose of Target into contact with bodily tissues or fluids. Additional Chemoreciprocals are TAG reagents (e.g., Target-Attaching Globulins). The TAG products are produced by bringing Target reagents into contact with body fluids for varying-periods of time .to form a comple and cleaving TAG therefrom. Two useful embodiments are S-TAG and F-TAG. A process for producing 5-TAG (SIow-Target-Attach- ing-Globul in) comprises reacting a blood serum or other body fluid with a Target product (e.g., Bromoacetyl- ce 11ulose-Mal i nin ) for approximately two hours OP- more at a low temperature, .e.g., 4°C, and cleaving S-TAG from the resulting material", e.g., with a dilute'acid, for approximately two hours at a temperature of 37°C. The product 5-TAG prepared in accordance with this process is characterized by being soluble in aqueous buffer solutions, forming a single line precipitate with its corresponding Recognin in Ouchterlony gel diffusion tests, being non-dialysable in cellophane membranes, being retained by millipore filters which retain mole¬ cules of over 1 0 , 000 molecular weight, having molecular weights in different states of aggregation as determined by thin layer gel chromatography of approximately 50,000 and multiples thereof into the macrog1obu1 in range, and having a spectrophotometr ic absorption peak wave-length o-f .280 mμ . A process for producing an F-TAG product (Fast- Target-Attach ing-Globul in) comprises reacting a blood serum or other body fluid with a Target reagent (e.g., Bromoacety1ce11ulose-Mal ignin) for approximately 10 minutes at a low temperature, e.g., 4°C, and cleaving F-TAG from the resulting material, e.g., with a dilute acid, for approximately two hours at a temperature of 37°C. The product F-TAG prepared in accordance with this process is characterized by being soluble in aqueous buffered solutions, forming a single line prec¬ ipitate with its corresponding Recognin in Ouchterlony gel diffusion tests, being non-dia1ysabIe in cellophane membranes, being retained by millipore filters which retain molecules over 25,000 molecular weight, having molecular weights, in different states of aggregation as determined by thin layer gel chromatography of approx- imately 50,000 and multiples thereof into the macro- globulin range, and having a spectrophotometr ic absorp¬ tion peak wave-length of 280 mμ. TAG products are useful for detecting cancer tumors in living mammals by determining the concentrat icms of S-TAG and F-TAG produced by a known volume of the mammal ' blood serum or other body. fluid and correlating this concentration with amounts determined to be indicative of cancer. TAG products are also useful for diagnosing the presence of tumor cells in an histology section, which comprises applying TAG conjugated with a labelling substance such as a dye or radioactive substance, to said section, whereby staining or radioactive labelling occurs only with tumor cells. TAG products have additionally been found to be cytotoxic to tumor cells. TAG products are also useful for directing the delivery of diagnostic, nutritional and therapeutic agents to specific cells or sites by introducing said agents in ' compiexed form with the TAG product. Normal cell division in plants or animals is restricted or inhibited when the cells come to occupy fully a particular space. The mechanisms (a) by which normal cells "recognize" that they have filled the space available to them, and (b) by which the operation of this recognition mechanism in turn inhibits cell division, have both been unknown. I have previously produced a group of compounds whose precursors are increased in concentration when normal recognition and learning occur, and which relate to recognition and learning in particles and cells, and with the connection of cells to each other. These compounds were termed Recognins. By attempting to produce these compounds fro normal cancer cells, the inventor has discovered that they are absent as such, and that changes in their molecular structure have occurred at the same time that the cancer cells have lost their ability (a) to recogniz that they have filled their normal volume, and/or (b) to stop dividing when they have filled their normal volume. Recognins are compounds with phys icochemical characteristics which mimic those configurations charact eristic of cancer cells in terms of their failure~to recognise and stop cell division. The use of Recognins goes beyond insight into the cancer mechanism, for irrmediate products and methods are thereby provided which are useful in the diagnosis and treatment of cancer, and for its prevention. I have discovered methods by which artificially cultured cells can be used to produce Recognins in particular Malignins. One advantage of the methods disclosed herein is that Recognins and the products derived therefrom can be manufactured efficiently in virtually limitless quantities. This discovery transcends the field of cancer research and is immediately applicable to any and all biological systems in which it is desired to influence growth and metabolism. Thus, by the manufacture of the particular compound or compounds of appropriate cell type in artificial culture, and the further. manufacture of products from these substances, a spec¬ ific influence may be brought to bear on any. tissue, cell, cell organelle, sub-organel 1e molecule or molecular aggregate in any living system. Thus, specific nutritional influences at critical times in development, specific diagnostic, preventative and treatment methods, and the construction of artificial bioelectr ical systems (as in tissue or organ transplants can all be affected. These artificial bioe1ectr ical systems can be made to bear the char cteristics of the . specific Recognin or Recognin chemoreciprocal of the normal tissue or component which they will neighbour, and thus avoid being "recognized" as "foreign" and thus avoid the usual reactions to alien substances, including rej ect ion . Another aspect of this invention is the production of a valuable specific ant ibody- 1 ike product (Anti- Astrocytin) to a specific brain product (Astrocytin), permitting the use of this ant ibody- 1 ike product -spec- ifically to complex with and, as a specific delivery vehicle to, specific points in the nervous "systems of various species. The TARGET-ATTACHING-GLOBULINS (TAG), are so named because they are produced by two reactions, the first reacting biological fluids with a synthetic complex containing phys icochemical configurations which mimic those of the MALIGNINS and called TARGET, the second, cleaving the specific TAG from the complex, and by the measure of the TAG so produced obtaining a quantitative indication from the biological fluids of living organ- isms as to whether there is present a tumor in that organism; and, hence, a diagnostic test for tumors. Because TAG products and ANTI-MALIGNIN are. physico- chemical ly complementary to MALIGNINS, they are termed CHEMORECIPROCALS. I have further previous l * discovered that two quant¬ itatively and qualitatively distinct TAG products can be produced depending upon the time permitted for the reaction of serum with the specific TARGET reagent used, and depending upon the time permitted for the cleavage of the product which has been compiexed. After examining the amounts of these products which could be produced from a number of different individuals with brain tumors and various other medical disorders, as well as in those with no apparent disease process, it became apparent that the amounts of these two new products which could be produced in a given individual was indicative of whether that individual had a malig¬ nant tumor, and hence provided a serum diagnostic test for malignant tumors. The uses of these products, in addition to their use in diagnos ing from serum and other biological fluids the presence of brain and other tumors, are illustrated by the demonstration that TAG and ant i -RECOGNIN compounds attach to glial tumor cel ls pre erentially in histolog- ical sections of brain tumor and surrounding tissue removed at surgery of the brain tumor. This preferential labelling by TAG and Ant i -RECOGNINS of tumor cells is demonstrated through standard inrmunof 1uorescent techniques. Thus a method is also available for deter¬ mining through histological examination with a new 5 degree of certainty whether tumor. cells are present in the tissue removed, and whether these tumor ceils have penetrated to the very edges of the tissue removed, indicating the likelihood that the tumor still remains in the brain or other organ, or that tumor cells are 10 absent from the periphery of the tissue removed, indicating the possibility that all of the tumor has been removed from the brain or other organ. In addition, the TAG and Anti -RECOGNINS produced as described have been found to be cytotoxic to glioma brain tumor cells 15 grown in tissue culture _uι vj ^ro. This high affinity for tumor cells in another medium, here grown in tissue culture, is further evidence of the specific-coupling potential of the new product TAG, and explains the adopt¬ ion of the name TARGET-ATTACHING-GLOBULINS (TAG) as do 20 TAG's properties in regard to the synthetic product TARGET, and to tumor cells in histological section. Further, the cytotoxicity of TAG and anti - RECOGNINS for tumor cells provides an additional new diagnostic test for the serum of patients who are suspected of suffering 25 from a tumor. Thus, for example, the serum or other body fluid of these patients is reacted with a TARGET reagent to produce TAG and the product TAG is tested in tissue culture growths of tumor cell ' s for cytotoxicity. Both e the concentration of TAG and. the degree of cytotoxicity 30 manifested by the TAG which can be produced from a given • 8 individual 's serum may be not only diagnostic but also of value in tracing the course of the disorder pre- operatively and postoperat ivel y in a given patient. The coupling of radioactive and dye tracers to TAG pro-vides 35 new TAG products which are useful j_n χJ_vo in the diag¬ nosis of tumors and in their exact localization. Thus, the injection of suitably labelled TAG either intra- arterially or intravenously, into the cerebrospinal fluid, or directly into brain tissue or its cavities, permits the demonstration by radioactive means, or by visualization of the coupled dye, of the presence of a brain tumor, for it is only to the tumor cells that the TAG specifically attaches. Further, this method permits the precise visualization of the location of the brain tumor. This can be seen to be an improvement of the ji y_iv_o diagnostic method Using anti-ASTROCYTIN produced in rabbit blood to label the brain tumor, because the use of TAG reagents produced from human serum avoids the possibility of foreign protein reactions Since the TAG reagents and anti-RECOGNINS have the chemical specificity which permits preferential attach¬ ment to ASTROCYTIN precursor containing tumor cells both I . XiJ-.---.-_l anc - in Σ_—_~ " these products may also be used therapeut ical 1y, as well as diagnost ical ly, when coupled, e.g., with radioactive, proton capture agents, or other toxic physical or chemical agents, so that these toxic substances may be localized preferentially through these compounds' specificity of attachment in the tumor cells as compared with their neighbouring normal ceils. This selectivity is universally recognised as the crucial, or at least one crucial factor for achieving effective chemical or physical therapy of tumors, and a factor which has hitherto not been achieved. Thus, the TAG product has demonstrated efficacy in attachin . prefer¬ entially ' to the tumor cells, and accordingly the propert- ies of a therapeutic agent. In the serum of patients with malignant tumors, as will be seen in the examples below, one type of TAG, SLOW-TAG (S-TAG) as distinguished from another type, FAST- TAG (F-TAG), can be produced in relatively greater amounts from a given volume of serum than in patients without such tumors. This- suggests that either one of OMPI the naturally occurring precursors. (P-TAG) of TAG is increased i concentration or that other factors exist which favour the relative _ ι _____ production of S-TAG over F-TAG. The possible relationship of the function of the actual synthetic products TARGET and TAG to their pre¬ cursors, and in turn to functions of postulated but not demonstrated cell "antigens" and circulating "anti¬ bodies." to them which may exist j_n y _vo has yet to be elucidated. Thus, for example, in ant ibody- 1 ike fashion, F-TAG and S-TAG produce single discrete lines of reactio with ASTROCYTIN in Ouchterlony gel diffusion, and the injection of TARGET into rabbits induces an increase in the yield of TAG products from-rabbit serum after reacting with TARGET. The finding that there may be a normal level of a precursor resembling circulating anti¬ body to a cell antigen which is hidden in the non- dividing cell raises a question as to the possible function of the pair. It is here proposed that TAG p_r_e_^ H- S.£-r. (P-TAG) and TARGET-like substances exist j_n vj ^ yo which function in the control of cell proliferation and cell death. Thus, for example, the exposure of a cel.1 constituent which normally is not directly exposed to serum proteins may occur during cell division. The exposure of this cell constituent may result in that constituent becoming converted to a TARGET-like substance to which the attachment of a P-TAG- like molecule from serum may then occur, which would stimulate cell division or al ternatively- inhibi t it. Alternatively, again-, a " non-dividing cell which is injured or malfunctioning may expose a TARGET-like substance to which the attachment of P-TAG-like molecules may be reparative. However, unde certain cell conditions, the attachment of P-TAG-like molecules may induce the destruction of the cell (e.g. ANTI -GLIGMA-TAG synthetically produced as here described is markedly cytotoxic to' glioma tumor cells growing in tissue culture). This may thus represent a mirror of a normal mechanism for the control of cell division, and for either the repair or the removal of individual cells in the body throughout the life of the organisms. If the exposure of cell constituents is abnormally increased so that abnormally large amounts of cell TARGET-like substances are formed, as may occur in rapidly dividing cancer cells such as in brain gliomas, an increase in the concentration of one type of serum P-TAG relative to another may be induced. Whatever the actual function of the precursors, the increase in the relative amount of predominantly one type of TAG, SLOW-TAG (S-TAG) which can be produced j_n yj_t o by the methods here described from the serum of patients with malignant tumors "is the basis of the serum diagnostic test described in the examples which follow. The ability according to the present invention to produce specific monoclonal species for S-TAG and F-TAG by means of new artificially produced cells as herein described, has permitted the separation of certain functions of these TAG molecules, which were previously not separable because they were mixed in the polyclonal form of their production. Thus, whereas the earlier TAG products had both the properties of preferential attach- ment to malignant cells and the cytotoxic property wherein the malignant cell is destroyed, the presently described monoclonal forms of TAG: MAMA-A and MAMA-B, - each demonstrate preferential attachment and thus specifi fluorescence with malignant cells but are not cytotoxic; in contrast, a mixture of MAMA-A and MAMA-B produces both fluoresence and cytotoxicity. The separation of diagnost ic and therapeutic uses is thus possible for the first t ime. The following examples illustrate the invention. - EXA PLi_i Production of Crude ASTROCYTIN-Precursor-Containing Fr ac t i on . Human brain glioma tumor tissue, removed at surgery, is dissected to be as free as possible of surface blood vessels and normal brain tissue. For a typical amount of dissected tumor tissue of II grams, the tissue is weighed into six 1.5 g. and two 1.0 g. aliquots. Each aliquot is then treated as follows. Each aliquot is homogenized in a neutral buffer solution by sonification or other mechanical means. For example, each aliquot is homogenized in 100 cc per g. of tissue of 0.005 M phosphate buffer solution, pH 7, in a Waring blender. Homogenizat ion should be done in the cold to prevent denaturing of the proteins present. For example, the blender should be βrecooled in a cold room at 0 - 5°C and operated for only three minutes. The homogenate is then centrifuged for clarific¬ ation, for example at 80,000 times gravity for 30 minutes in a refrigerated ul tracentr i uge. The soluble supernat¬ ant is decanted and kept in the cold. The insoluble residue is rehomogenized with a further 100 cc of neutral buffer and centrifuged as before, and the second soluble extract combined with the first. Best yields are obtained when this procedure of homogenizat ion and centrifuging is repeated until less than 50 micrograms of protein per ml. of solution are obtained in the supernate. With most tissues this is accomplished by the fifth extraction. The solutions thus obtained are combined and concentrated by perevaporat ion with subsequent dialysis, as by dialysis against 0 . 005 M phosphate buffer in the cold to produce a volume of 15 ml. The volume of this solution is noted, an aliquot is taken for total protein analysis, and the remainder is fractionated to yield the protein fraction having a pK range between I and 4. The preferred method of f ract i onat ion is chromatography as foil ows . The solution is fractionated in the cold room (4°C) on a DEAE cellulose (Cellex-D) column 2.5 x 11.0 cm., which has been equilibrated with 0.005 M sodium phosphate buffer. Stepwise eluting solvent changes are made with the following solvents (solutions): Solution (1): 4.04 g. NaH^O^. and 6.50 g. Na_HPO. are dissolved in 1 5 litres of distilled H_0 (0.005 molar, pH 7); Solution (2): 8.57 g. NaH 2 PO^, are dissolved in 2480 ml. of distilled H 2 0; Solution (3): 1-7.1 g. of NaH 2 PO^ are dissolved in 2480 ml. of distilled H 2 0 (0.05 molar, pH 4.7); Solution (4): 59.65 g. of NaH 2 PO. are dissolved in 2470 ml. distilled H 2 0 (0.175 molar); Solution (5): 101.6 g. of aH 2 PO ψ are dissolved in 2455 ml. distilled H 2 0 (0.3 molar, pH 4.3; Solution (6): 340.2 g. of NaH 2 PO. are dissolved in 2465 ml. of distilled H 2 0 ( 1 . 0 molar, pH 4.1); Solution ' (7) : 283.64 g. of 80% phosphoric acid (H 3 PO^) are made up in 2460. ml. of distilled H_0 (1.0 molar , pH 1.0) . Add nervous tissue extract, 6 to 10 ml. volume. Let it pass into column. Then, overlay with Solution (1) and attach a reservoir of 300 mi. of Solution (1) to drip by gravity onto the column. Three ml. aliquots of effluent are collected by means of an automatic fraction collector. The subsequent eluting solutions are exchanged stepwise at the following eiution tube numbers. Solution (2): at tube 88, bring solution on column to top of resin, then overlay and attach reservoir of 50 ml. of Solution (2); Solution (3): at tube 98, bring solution of column to top of resin, then overlay and -attach reservoir of 75 ml. of Solution (3); Solution (4-): at tube 114, bring solution on column to top of resin, then overlay and attach reservoir of 150 mi. of Solution (4); Solution (5): at tube 155, bring solution on column to top of resin, then overlay and attach reservoir of 125 ml. of Solution (5); Solution (6): at tube 187, bring solution on column to top of resin, then overlay and attach reservoir of 175 ml. of Solution (7); continue ei'Uting until, at tube 260, eiution is complete. Use freshly prepared resin for every new volume of tissue extract. Each effluent tube is quantitatively analysed for protein. The eluates in the tube numbers 212 to 230 are combined, and contain the* crude products from which ASTROCYTIN will be produced While data have been published on this crude material, called fraction 10B in the past (P_ o ej Meta- froiiu I -D_°-_i-l-._y_?.£ . ϋ!_-!Y..siejJJ - PP• 555-569 (Plenum Press, 1970); Journal of Neurosurgery, Vol. 33, pp. 281 - 286 (September, 1970)), the cleavage from fraction- 10B. can be prepared as a product in amounts between 0.1 and 10 mg. per gm. of original fresh nervous system tissue from which it was obtained. In addition to an ASTROCYTIN- precursor, it contains varying amounts of covalently bound carbohydrate residues including a number of hexoses, namely glucose, galatose, mannose; hexosamines, including glucosamine, galatosamine and mannosamine; and occasionally other sugars, such as fucose, ribose and perhaps rhamnose. It also contains large molecular weight protein products and several lipids and nucleic acids. EXAMPLE_2 Production of Purified ASTROCYTIN from Crude ASTROCYTIN- Precursor-Containing Fraction. The ASTROCYTIN-Precursor-Containing fraction is further isolated from contaminants. In a preferred embodiment, the material from Example 1 is chromatograted -on Sephadex G-50 resin with a typical column 40 cm. long, 2.5 cm. in diameter, and 196 ml. in volume. The pressure used is 40 rrm. H . ; the flow rate is 35 ml. per hour, .and the buffer is 0.05 molar phosphate buffer solution, pH 7.2. The first ( f 1 ow-through) peak contains ASTROCYTIN-Precursor together with impurities, whereas subsequent peaks contain only impurities. In the preferred embodiment, the products in the above first f 1 ow-through -peak are concentrated on Sephadex G-15, and then passed onto a column of Cellex-D with the same solutions, (1) to (7), as in Example 1, and the same eiution steps as are performed in Example 1. The product ASTROCYTIN is present as a sharp peak in the same tubes (numbers 212 - 230) as before, thus maintain¬ ing its behaviour on Cellex-D chromatography without the presence of a large number of contaminants. Low molecular weight contaminants may then be removed by techniques known to the art, such as mi 11 i - pore disc filtration. In the preferred method, the product ASTROCYTIN is freed of salt and other small mole¬ cular weight contaminants by filtration through Millipore Pell icon Disc No. 1000, 13 mm., which retains substances of molecular weight greater than 1000 and permits the passage of those of molecular weight below 1000. The product ASTROCYTIN remains on the Pell icon Disc, and is recovered from it by washing with Solution (1) of Example 1. ASTROCYTIN is then obtained by isolating the compoun having a molecular weight of 8000 from the above solution. A preferred method uses thin layer gel (TLG) chromato¬ graphy as follows: The apparatus used is the corrmercial ly available one designed by Bochringer Mannheim GmbH; Pharmacia Fine Chemicals and CAMAG (Switzerland). The resin (2.5 g. of Sephadex G-200 superfine) is prepared in 85 ml. of 0.5 M. NaCl in 0.02 M Na^PO^KH^O^. Phosphate Buffer, pH 6.8 (6.6 - 7 . 0 ) . Allow to swell for two or " three days at roo temperature with occasional gentle mixing (magnetic and other stirrers should not be used). The swollen gel is stabilized for three weeks at refrigerator temperature; however, bacterial and fungal growth may interfere with the swollen gel. If the gel is to be kept for longer periods of time, a small amount of bacter iostatic agent should be added (sodium azide, 0.02%). 2.5 g. of dry gel are used to make two 20 x. 20 cm. glass plates 0.5 nrm. thi The plates. are allowed to dry at room temperature for [ 0 minutes and transferred to a moist chamber where they can be stored for two weeks, or they are used irrmediately after appropriate pre-equi I ibration (usually during the night for a minimum of 12 hours). The main function of equilibration is to normalize the ratio between the stationary and modile phase volumes. With the pre- equilibrated plates in a horizontal position, substances to be determined are applied with micropipettes as spots or as a streak at the start line. 10 ml. to 20 ml. of 0.2 - 2% protein solution are placed on the edge of a microscopic coyer slide (18 x 18 rrm. ) and held against the gel surface. In a few. seconds, the solution will soak into the gel. All samples'are first prepared on the cover slides and then quickly applied. If not enough material is used, it is difficult to locate individual spots after separation-. If too much material is applied, no defined separation occurs. The samples are diluted with buffer for easier handling and the separation of samples is carried out in a descending technique with the plate at an angle of 22°. The flow rate of 1 - 2 cm/hour is most suitable. Marker substances (such as cytochrome C, haemoglobin, myoglobin or bromophenol Blue labelled albumin) are applied at different positions across the plate to give a check on possible variations of flow across the plate and also to serve as reference proteins for the calculation of the relative distance (mobility) of unknowns. After ' the application of samples, the plates are replaced in the apparatus and the paper wick is pushed slightly downwards to ensure good contact with the gel layer. The paper wick must not drip. Excess moisture is wiped off. The liquid solvent in the reservoir is kept constant at 1 cm. from the upper . _ end of the vessel. The runs are usually completed in 4 to 7 hours depending on the progress of separation. With coloured substances, separation follows directly. The separated spots of protein are easily made visible by transferring them to a paper -sheet replica of TLG plate after the chromatographic separation has been completed, and by staining them on the prewashed methanol + R-0 + acetic acid - 90:5:5, for 48 hours. The paper sheet is 3 nrm. filter paper. A sheet of paper 20 x 18 cm. is placed over the gel layer and pressed •( rol led) just enough to ensure contact with the gel. Care is taken not to trap air under the paper (replica) and not to disturb the gel layer. The liquid phase is soaked off from the gel layer by the paper and removed after one minute, irrmediately dried in an oven at 60°C for 15 minutes and stained in the normal way with any of the routine staining procedures. Staining is performed by spraying the replica-paper with 0.03% diazotized sulfan- ilic acid in 10% Sodium Carbonate (Pauley's Reagent). Staining can also be accomplished with a saturated solution of Amido Black in Methanol -Acet ic Acid (90:10y/v is used); the staining time is 5 - 10 minutes. For de- staining, rinse with two volumes of 90:10 methanol and acetic acid solution mixed with one volume of H_0. It is difficult to obtain low background staining without very extensive washing. The plates themselves may also be dried at 60°C (in an oven with air circulation) but only if the ASTROCYTIN is to be stained. For isolation purposes, the plate should only be air dried at room temperature. Over-heating can lead to cracking, but this can usually be avoided with a 50° - 60°" temperature whic - dri.es a Sephadex G-200 plate in 15 - 30 minutes. The dry plates are allowed to swell for 10 minutes in a mixture of methanol + H 2 0 + acetic acid (75:20:5) and stained in a saturated Amido Black in the same solvent system for five hours and subsequently washed by bathing for two hours in the same solvent before they are dried. Tor molecular weight determinations, the distance from the starting line to the middle of each zone is measured with an accuracy of 0 . 05 nrm. either directly on the print (replica) or on the densitogram. The result is expressed by the Rm value defined as the ratio of the migration distance of the tested protein (d ) to that of cytochrome C or myoglobin (d ) which is used as the reference protein: Relating migration distance of tested substance to standard is the formula (-Rm = _£). A straight calibration line is obtained by m plotting the og ι _iJ τm of the molecular weight of the standards used ag ~ ainst the Rm„ values. From this line,' the molecula weight of the unknown protein can be obtained. For most exact results, mix six equal parts of the protein sample solution with standard, in this case, Cytochrome C, befor applying to the plate. By the above TLG procedure, the product ASTROCYTIN is observed as a discrete spot at a distance of approximately 0.83 +_ 0 . 02 with reference to the standard Cytochrome C, yielding an approximate mole¬ cular weight of 8000 for ASTROCYTIN. Several discrete products are separated in this procedure fromASTROCYTIN on the basis of slight differences in chemical structure and large differences in molecular weight. Thus, three products carried as contaminants to this point with mole¬ cular weights of approximately 64,000, 148,000 and 230,000 respectively, and one occasionally of mol.ecular weight 32,000, have been detected and removed by the TLG methods described above. The product ASTROCYTIN is aspirated with the gel in which it is contained,, in dry form, dissolved in Solution (1) and freed of resin by centrifuging or other similar means. The product ASTROCYTIN which has been produced at this stage is soluble in distilled water, soluble at neutral and acid pH, and insoluble at alkaline pH and has a spectrophotometr ic absorption peak wavelength of 280 mμ. It is a polypeptide with molecular weight, as stated above of approximately 8000. Its covalently linked aminoacids are shown by hydrolysis with 6N HC1 , then quantitative automatic determination to have the following average composition of aminoacids: Approximate Number of_„R_ e .. S .Λ $u,e_s_ Aspartic acid 9 Threonine 5 Ser ine 6 Glutamic acid 13 Prol ine 4 Glycine 6 Alanine 9 Valine , 4 1 /2 Cyst ine 2 Methionine 1 Isoleucine 2 Leucine 8 Tyros ine 2 Phenylalanine 3 Lysine 8 Histidine 2 Arginine 4 Approximate Total 88 Cysteic acid, hydroxyprol ine, norleucine, ammonia, i sodesmos ine, desmosine, hydroxy1ys ine, 1ysinonorleucine and ganrma-aminobutyr ic acid are all absent in detectable amounts, but a trace of glucosamine may be present. From 11 grams of the starting brain tumor tissue in Example 1, approximately 3 g. of purified ASTROCYTIN are produced by the above methods. EXA PLE_3 Production of MALIGNIN-Precursor in Artificial Cancer Cell Cu1 ture. Generally, a sterile technique is scrupulously maintained. All solutions (e.g.-Hank's Balanced Salt (BSS), F-10 OMPI Nutrient medium, foetal calf serum, trypsin solution) ar incubated at 35°C in a water bath for approximately 20 minutes or more before use. Cells are removed from tumor tissue and grown _n yii££ f° r rnany generations using a suitable medium, such as that described below. Pre-rinse beakers to be used with a sterilizing solution, for example, 12-propanol plus Amphyl or creoline solution. In the preferred embodiment, the artificial cancer cells (i.e., cells grown _n vit£θ for many generations) are grown in 250 ml. flasks. The liquid medium in which the cells are growing is discharged into the pre-rinsed beakers. The cells are then washed gently with 5 - 10 ml. of Hank's BSS or other simijar solution for 30 seconds. Avoid agitation. All walls and surfaces are washed. The solution is clarified of cells by centrifug¬ ing in the cold from 10 minutes at 3,000 rpm. The medium is poured into a beaker as above. Add a small amount of buffered proteinase enzyme solution and rinse quickly to avoid digestion of the cells. In the preferred method, 1 - 2* ml . of trypsin solution (EDTA) are added and rinsed for only 10 seconds. Pour off the trypsin solution. Add a similar volume of fresh trypsin solution and incubate until the cells are seen to be separated from the walls of the chamber through microscopic observation. This usually requires 5 - 10 minutes. Add a suitable growth medium, such as 50 ml. of a solution of 7 - 10 percent solution of foetal calf serum in 100 ml. of F-10 Nutrient medium. - Twenty five ml. of the fresh medium with cells are transferred to a new growth chamber for propagation and the remaining 25 ml. are kept in the first chamber for propagation. Both chambers are placed in an incubator at 35°C for approximately seven days. By the procedure of this Example to this point, an artificial cancer cell culture is divided into two fresh cultures approximately every seven days. This entire procedure may be repeated as often as desired, at approximately seven-day intervals for each growth chamber. Thus, the number of cells growing _πτ vi_t£o may be approximately doubled every seven days. The cells may be extracted for the production of MALIGNIN after approximately seven days of growth. For example, cells growing in each 250 ml. growth chamber as 'described above may be recovered as follows: The medium is transferred to a centrifuge tube and centri¬ fuged at 3,000 rpm in the cold for 10 minutes. The medium is discarded. The cells remaining in the growth chamber are scraped from the ch.atr.ber walls and washed into the centrifuge tubes with neutral buffer solution. The cells are washed twice with neutral buffer solution and centrifuged again at 3,000 rpm in the cold, and the medium is discarded. The washed cells are suspended in 10 ml. of neutral phosphate buffer until ready for extraction of the crude MALIGNIN-Precursor-Containing fraction. EXAMPLE_4 Production of Crude MALIGNIN-Precursor-Containing Fractio Washed cells suspended in neutral buffer from Example 3 are mechanically disrupted under conditions which avoid the denaturing of most proteins. In the preferred method, the washed cells are treated in the cold with a sonifier for 20 seconds. After soni f icat ion " , the cell residues are cent-, rifuged at 30,000 rpm for 30 minutes and the supernatant is decanted. Ten ml. aliquots of buffer solution are used to wash remaining cells from the chamber and these are added to the remaining cell residues. Sonify and centrifuge as above and combine the supernatants . Repeat the process once more. The combined supernatant is perevaporated to reduce the approximate 30 ml. volume to 6 - 7 ml . An aliquot is taken for total protein analysis and the remainder is fractionated according to the methods of Example 1 for ASTROCYTIN Precursor. ________! Production of Purified MALIGNIN Product from Crude MALIGNIN-containing Fraction. The product MALIGNIN is further isolated from contaminants by the methods of Example 2 for ASTROCYTIN. In the TLG step of the preferred embodiment, the product MALIGNIN is observed at a discrete spot at a distance of approximately 0.91 + 0.02 with reference to the standard cytochrome C, yielding an approximate molecular weight of 1 0 , 000 for MALIGNIN. The product MALIGNIN which has been produced at this stage is soluble in distilled water, soluble at neutral or acid pH, and insoluble at alkaline pH and has a spectrophotometr ic absorption peak of 280 mμ. It is a polypeptide with a molecular weight of approximately 10,000. Its covalently linked aminoacids are shown by hydrolysis with 6N HCl then quantitative determination to have the following average composition of aminoacids: Approximate Number of Res idues Aspart ic Acid 9 Threonine 5 Ser ine 5 Glutamic Acid 13 Pro 1 i ne Glycine 6 Alanine 7 Valine 6 I/2 Cystine 1 Meth i on i ne 2 I so1eucine 4 Leucine 8 Tyros ine - 3 Phenylalanine 3 .. Lys ine 6 Hi st idine 2 Arginine 5 Approximate Total 89 the aminoacids cysteic acid, hydroxyprol ine, norleucine, arrmonia, isodesmosine, desmosine, hydroxylys ine, 1ys inonorleuci ne and garrma-aminobutyr ic acid being absent in detectable amounts. A typical yield of pure MALIGNIN from twelve 250 ml. reaction chambers of Example 3 together is approximately -1 mg. of MALIGNIN. EXAMPLE_6 Hydrolytic Cleavage of * RECOGNINS. A solution of RECOGNIN, in this case either Astrocytin or Malignin at pH between 1 and 2 is allowed to stand in the cold. After 7 to 14 days, TLG chromat¬ ography shows the product to have been reduced in mole- cular weight by approximately 200. When the solution is allowed to stand longer, further ' units of approximately 200 molecular weight are cleaved every 7 to 10 days. Thus, with Astrocytin the molecular weight is reduced fro 8,000, and with MALIGNIN the molecular weight is reduced from 10,000 in each case by units of approximately 200 sequential 1y. The phys icochemical specificities of ASTROCYTIN are retained by each product down to approximately 4,000 molecular weight. The phys icochemical specificities of Malignin are retained by each product down to approx¬ imately 5,000 molecular weight. This is shown by Ouchterlony gel diffusion tests against Anti-Astrocytin and Anti-Ma1 ignin, respectively. This cleavage can also be accomplished enzymat- ically, as with trypsin and other proteinases, with s imi 1 ar resul ts . The molecular weights of these compounds prepared by hydrolytic cleavage of RECOGNINS may be approximately defined by the following formulae: For products having the phys icochemical specificities of Astrocytin: 4000 + 200x = Y For products having the phys icochemi cal specificities of Malignin: 5000 + 200x = ' Y wherein Y is the molecular weight of the product and X is 0 or an integer from 1 to 19. _________ Production of Artificial Tissue or Organ with RECOGNINS. A rigid walled tube of plastic, metal, or other suitable rigid material is dipped in or impregnated with a highly concentrated (i.e., lO. g/ml.) viscous solution of RECOGNIN, in this case either Astrocytin or Malignin, until all surfaces are fully coated with the RECOGNIN. Alternately, RECOGNIN solution is passed through and around the tube under pressure until all surfaces are fully coated. The tube is then dried in air or _n vacuo, or lyophilized. The process of coating is repeated several times in order to build up multiple molecular layers of RECOGNIN coating. The tube is now ready to be placed in a cavity or in a tissue which contains Astrocytin- or Malignin- like precursors in the neighbouring tissue or fluid of a living mammal. This artificial tissue or organ may be used to minimize or to eliminate reactions which foreign substances without the RECOGNIN coating would incite. Artificial tissues or organs of other geometries may similarly be produced. EX.AMPLE_8 Production of TARGET reagents from RECOGNINS. ASTROCYTIN, prepared as in Example 2 above, or MALIGNIN, prepared as in Example 5 above, is compiexed with a carrier to produce a TARGET reagent. In the preferred embodiment, ASTROCYTIN or MALIGNIN is dissolved in 0.15 M. NaH PO^ - citrate buffer, pH 4.0. A bromoacety1-resin, for example bromoacetylcel lulose (BAC) having 1.0 to 1.5 milli- equivalents Br per gram of cellulose, stored in the cold, is prepared in 0.15 M NaH^O^, buffer, pH 7.2. Convert the buffer to pH 4 by pouring off the pH 7.2 buffer solution and adding 0.15 M NaH_PO^ - citrate buffer, pH 4.0. The ASTROCYTIN .or MALIGNIN solution and the BAC solution are stirred together (10:1 BAC to RECOGNIN ratio for 30 hours at room temperature, and then centrifuged. It is preferred that all sites on the BAC which are available to bind to RECOGNIN be bound. This may be accompl i she " d as follows. The supernatant from the irrmediately preceding step is l.yophilized and the protein content determined to indicate the amount of ASTROCYTIN or MALIGNIN not yet compiexed to BAC. The compiexed BAC- ASTROCYTIN (or BAC-MALIGNIN) is resuspended in 0.1 M bicarbonate buffer pH 8.9 and stirred 24 hours at 4°C to permit the formation of chemical bonds between the BAC and the ASTROCYTIN or MALIGNIN. After the 24 hours, the suspension is centrifuged and ' the supernatant is analysed for protein. The compiexed BAC-ASTROCYTIN or BAC-MALIGNI is now resuspended in 0.05 M aminoethanol - 0.1 M bicarb¬ onate buffer, pH 8.9 in order to block any unreacted bromine. The suspension is centrifuged, and the super- natant is kept but not analysed because of the presence of aminoethanol. Removal of all unbound ASTROCYTIN or MALIGN-IN is then accomplished by centrifuging and re- suspension .for three washings in 0.15 M NaCl until no absorbence is measured on the spectrophotometer at 266 mμ The BAC-ASTROCYTIN or BAC-MALIGNIN complex is now stirred in 8 M urea for 2 hours at 38°C, centrifuged, then washed (three times usually suffices) with 8 M urea until no absorbence is shown in the washings at 266 mμ. The compl is washed twice with 0.15 M NaCI to rid it of urea. The complex is then stirred at ' 37°C in 0.25 M acetic acid for 2 hours to demonstrate its stability. Centrifuge and read supernatant at 266 mμ -- no absorbence should be present. This chemically compiexed BAC-ASTROCYTIN or BAC-MALIGNIN is therefore stable and can now be used as a reagent in the methods described below; in this stable reagent form, it is referred to as TARGET (TOPOGRAPHIC-ANTIGEN-LIKE-REAGENT-TEMPLATE) because it is a synthetically produced complex whose physical and chemical properties mimic the stable cell-bound pre- cursor of ASTROCYTIN or MALIGNIN when it is in a potential reactive state with serum components. .For storing, TARGET reagent is centrifuged and washed until neutralized with 0.15 M NaH 2 PO. buffer, pH 7.2. TARGET reagents may be» prepared from bromo- acetyl liganded carriers other than cellulose, such as bromoacety1ated resins or even filter paper. EXAMPLE_9 Production of antisera to Astrocytin, Malignin and TARGET Antisera to Astrocytin, Malignin or TARGET reagents may be produced by inducing an antibody response to them in a mammal. The following procedure has been found to be satisfactory. One m . of RECOGNIN (Astrocytin or Malignin) is injected into the toe pads of white male rabbits with standard Freund's adjuvant, and then the same injection is made intraper i toneal 1y one week later, again intra- peritoneally ten days and, if necessary, three weeks later. ' Specific antibodies may be detected in the blood serum of these rabbits as early as one week to ten days after the first injection. The same procedure is followe for TARGET antigen by injecting that amount of TARGET which contains 1 mg. of Astrocytin or Malignin as deter¬ mined by a Folin-Lowry determination of protein. The speci f ic ant ibody to Astrocytin is named Ant i -Astrocyt in. The specific antibody to Malignin is named Ant i -Mai ignin. Similarly, the specific antibody to TARGET reagent is named Anti -Target. These antibodies show clearly on standard Ouchterlony gel diffusion tests for antigen-antibody reactions with specific single sharp reaction lines 5 produced with their specific antigen. The presence of specific antibodies in serum can also be tested by the standard quantitative precip- itin test for antigen-antibody reactions. Good quant¬ itative precipitin curves are obtained and the micro- 10 grams of specific antibody can be calculated therefrom. -Further evidence of the presence of specific antibodies in serum can be obtained by absorption of the specific antibody Anti-Astrocytin onto Bromoacetyl- cel 1ulose-Astrocytin (BAC-Astrαcyt in) prepared above. 15 The anti serum-containing specific Anti-Astrocytin can be reacted with BAC-Ast ocytin. When the serum is passed over BAC-Astrocyt in only, the specific antibodies to Astrocytin bind to their specific antigen Astrocytin. Since Astrocytin is covalently bound to Bromoacetyl- 20 cellulose, the specific antibody, Anti-AstrocytIn, is now bound to BAC-Astrocyt in to produce BAC-Astrocyt in- Anti -Astrocytin (BACA-Antϊ -Astrocytin) . This is proved by testing the remainder of the serum which is washed free from BAC-Astrocyt in. On standard ' Ouchter lony 25. diffusion no antibodies now remain in the serum which will react with Astrocytin. It is therefore concluded that all specific antibodies (Ant i -Astrocytin) previously shown to be present, in the serum, have been absorbed to BAC-Astrocyt in. Furthermore, when Anti-Astrocytin is 30 releaed from its binding to BAC-Astrocyt in, it is thereby isolated free of all contaminating antibodies. This release of Anti -Astrocytin may be accomplished by washing the BACA-Ant i-Astrocyt in complex with 0.25 M acetic acid (4°C. , 2 hrs.) which has been shown above not to break 35 the BAC-Astrocyt in bond. Still further evidence of the presence of specifi * BURI antibodies in serum can be obtained by adsorption of the specific antibody Ant i -Mai ignin onto Bromoacetyl- ce11u1ose-Mal ignin (BAC-Mal ignin) prepared above. The antiserum containing specific Anti-Mai ignin can be reacted with BAC-Mal ignin. When the serum is passed over BAC-Mal ignin , only the specific antibodies to Malignin bind to their specific antigen Malignin. Since Malignin is covalently bound to Bromoacety1 -ceϊ 1u1ose, the specific antibody, Ant i -Mai ignin, is now bound to BAC-Mal ignin to produce BAC-Mal ign in-Ant i -Mai ignin (BACM-Anti -Mai ignin) . This is proved by testing the remainder of the serum which is washed free from BAC- Malignin. On ' standard Ouchterlony diffusion no anti¬ bodies now remain in the serum which will react with Malignin. It is therefore concluded that all specific antibodies (Ant i-Mai ignin) previously shown to be present in the serum, have been absorbed to BAC-Mal ignin. Furthermore, when Ant i-Mai ign in is released from its binding to BAC-Mal ignin , it is thereby isolated free of all contaminating antibodies. This release of Anti- Mai ignin may be accomplished by washing the BACM-Anti- Malignin complex with 0.25 M acetic acid (4°C, 2 hrs.), which has been shown above_ not to break the BAC-Mal ignin bond. The antibodies to TARGET show clearly on stand¬ ard Ouchterlony gel diffusion tests for antigen-antibody reactions with specific single reaction lines produced with TARGET which show a line of identity with the line of reaction to ANTI-ASTROCYTIN or ANTI-MALIGN-IN antisera (i.e., that produced to the injection of ASTROCYTIN or MALIGNIN themselves). Some rabbits, it has been noted, have levels of ANTI-TARGET in their blood prior to being injected with TARGET. These ANTI-TARGET substances, when reacted specifically with TARGET reagent as descrFbed below in tests of human sera, lead to the production of approximately equivalent amounts of the two types of TAG, S-TAG and F-TAG (see later Examples). EXAMPLE_!P_ Detection of Malignant Tumors by Quantitative Product¬ ion £n virro of TARGET-ATTACHING-GLOBULINS (TAG) from Biological Fluids. TARGET reagent prepared in accordance with Example 8 is washed to remove any unbound RECOGNIN which may be present due to deterioration. The following - procedure is satisfactory. TARGET reagent is stirred with acetic acid for two hours at 37°C, centrifuged, the supernatant decanted, and the optical density of the supernatant read at 266 mμ. If there is any absorbence, th ' is wash is repeated until no further materia is solubilized. The TARGET is .then resuspended in phos- phate buffered saline, pH 7.2. (standard S-TAG and F- TAG purified from previous reactions of human serum by the procedure described below can be used if available, as reference standards to test the TARGET reagent, as can whole rabbit serum which has been determined to contai S-TAG and F-TAG by other TARGET preparations). The Slow-Binding (S-TAG) determination is per¬ formed as follows: Frozen serum stored for more than a few days should not be used. Serum is carefully prepared from freshly obtained whole blood or other body fluid by standard procedures in the art. The following procedure has been found to be satisfactory. Blood is allowed to clot by standing for 2 hours at room temperature in a glass test tube. The clots are separated ' from the walls with a glass stirring rod, and the blood is allowed to stand at 4°C for a minimum of 2 hours (or overnight). The clots are separated from the serum by centrifuging at 20,000 rpm at 4°C for 45 minutes. The serum is decanted into a centrifuge tube and centrifuged again at 2000 rpm at 4°C for 45 minutes. The serum is decanted and an 1% Solution of Methiolate- (Ig. in 95 ml. water and 5 mi. 0.2 M bicarbonate buffer pH 10) is added to the extent of 1% of the volume of serum. Serum samples, prepared by the above or other procedures, of 0.2 ml each are added to each of 0.20 ml. aliquots of TARGET suspension reagent containing 100- 200 micrograms of RECOGNIN per 0.20 ml. of TARGET re¬ agent, in duplicate determination. The suspension is mixed at 4°C in such a manner as to avoid pellet form¬ ation. For example, ' a small rubber* cap rapid shaken may be used for 1 - 2 seconds and then, with the tubes • slightly slanted, they may be shaken in a Thomas shaker for 2 hours or more. The TARGET reagent and protein bound to it are separated from the serum. One of the procedures which has been found to be satisfactory is the following. The tubes are centrifuged at 2000 rpm for 20 minutes at 4°C, the supernatant is decanted, the pellet which is formed by centrifuging is washed 3 times by remixing and shaking at room temperature with 0.2 - 0.3 ml. of 0.15 M Saline, and centrifuged and the super- natants are discarded. The protein which remains attached to the TARGE is cleaved therefrom and quantitatively determined. For - example, 0.2 ml. of 0.25 M acetic acid is added, the suspension is shaken for 1 to 2 seconds with a rubber cap shaker, and then in a Thomas shaker for 2 hours in a 37°C "i ncubator . The tubes are centrifuged at 2000 rpm at 4°C for 30 minutes. The supernatant is carefully decanted to avoid transferring particles and the optical density of . the supernatant is read at 280 mμ . The value of the optical dens'it — is- d-ivi-ded by a factor of 1.46 for results in micrograms per ml. of serum protein (S-TA Duplicate determinations should not vary by more than 5%. Any other procedure effective for determining protein content may be used, such as Folin-Lowry determination, but standards must be specified to determine the Fange of control and tumor values of S-TAG and F-TAG concent¬ ration. - The Fast-Binding (F-TAG) determination is performed as follows: Frozen serum stored for more than a few days should not be used. Serum is carefully prepared from freshly obtained whole blood or other body fluid by standard procedures in the art. The procedure given above in this Example for serum preparation is sat i sfactory. Serum samples, prepared by the above or other procedures are allowed to stand at °C for 10 minutes less than the total time the S-TAG serum determinations were allowed to be in contact with TARGET reagent above ' (e.g., 1 hour 50 minutes if a "two hour" S-TAG determin¬ ation was made). This procedure equilibrates the temperature histories of S-TAG .and F-TAG determinations. Add 0.2 ml. samples of the temperature equil¬ ibrated serum to each of 0.20 ml. aliquots of TARGET suspension reagent containing 100 - 200 micrograms of RECOGNIN pe 0.20 ml. TARGET reagent, in duplicate deter¬ mination. The suspension is then mixed at 4°C for approximately 10 minutes in such a manner as to avoid pellet formation. For example, a small rubber cap rapid shaker may be used for 1 - 2 seconds and then, with the tubes slightly slanted, they may be shaken in a Thomas shaker for approximately 10 minutes. The TARGET reagent and protein bound to it are separated from the serum. One of the procedures which has been found to be satis¬ factory is the following. The tubes are centrifuged at 2000 rpm for 20 minutes at.4°C, the supernatant is decante the pellet which is formed by- centrifuging is washed 3 times by remixing and shaking at room temperature with 0.2 - 0.3 ml. of 0.15 M Saline and centrifuged, and the supernatants are discarded. The protein which remains attached to the TARGET is cleaved therefrom and quantitatively determined. The procedure described above in this Example for determining S-TAG concentration is satisfactory. Any other procedure effective for determining protein content may be use , such as Folin-Lowry determination, but standards must be specified to determine the range of control and tumo values of S-TAG minus F-TAG concentration. The final results are expressed as TAG micro¬ grams per ml. of Serum, and equal S-TAG minus F-TAG. TAG values in non-brain-tumor patients and other control currently range from zero (or a negative number) to 140 micrograms per ml. of serum. TAG values in the first patients studied, brain tumor patients, ranged from 141 to 500 micrograms per ml. of serum. In the first "blin study of 50 blood samples conducted according to the procedures of this Example utilizing TARGET reagent prepared from Astrocytin and bromoacety1ce11u1ose, 11 out of 11 brain tumors and 28 out of 32 normals were correct identified. One of the 4 supposed normals (i.e., non- brain tumor controls) turned out to have a cancer of the thyroid gland which had* apparently been successfully treated some years before. The three remaining normals were individuals aged 60 - 70 who were in poor health, possibly having nondiagnosed cancer. Of the remaining 7 samples, three out of three cases of Hodgkin's Disease were correctly identified; one sample in the tumor range (141 - 500 μg. TAG/ml . ) corresponded to patients having respectively, an intracranial mass diagnosis uncertain but non-tumor, and osteosarcoma (non-brain tumor) and a melanotic sarcoma (non-brain tumor). Subsequent blind studies conducted according to the procedures of this example utilizing TARGET reagent prepared from MALIGNIN and bromoacetylcel lulose correctl identified three out of three malignant brain tumors and all normals, then were continued as detailed in Example 10A which fol lows . EXAMPLE_ θA Determina ion of Ant i -Ma1 ignin Antibody in 1,026 Cancer Patients and Controls: A Seven-Year Nine Hospital Blind S tudy . The antibody to malignin, a cancer cell 10,000 Dalton polypeptide of known composition, was quantitat¬ ively determined blind by specific irrmunoadsorption in 1,094 serum specimens from 1,026 cancer patients and controls. The concentration of anti-malignin antibody, known to be cytotoxic to cancer cells j_n vi_t£o, was elevated in 92 . 7% of sera from patients with clinically and pathologically active cancer (mean 273.7 + 156.5 micrograms/ml ) compared with healthy normal subjects (mean 59.1 + 27.0 micrograms/ml ) in a broad range of types of malignancy in support of the hypothesis that malignin is a general transformation antigen. The anti¬ body was in the normal range (0 - 134 micrograms/ml ) in 100% of sera of healthy normal subjects (first control group), in 94.6% of sera of out-patient hospital non- cancer controls (mean 64.3. +_ 46.3 micrograms/ml ) (second control group) and in 91.2%of sera of in-patient medical- surgical disorder non-cancer patients (mean 81.2 + 67.3 micrograms/ml ) (third control group). That only an activ cancer state appears to be associated with elevated anti¬ body levels is supported by the finding that the antibody was in the normal range in 94.2% of sera from cancer patients who had been successfully treated and clinico- pathologically showed 'no evidence of disease' at the time of the determination (fourth control group) (mean 70.1 •+ 36.7 micrograms/ml ) . None of the four control groups was statistically significantly different from any one- other, but each control group differed from the activ cancer group at a level of P <0.000001. Of the 109 cance patients who had antibody levels below 135 micrograms/ml , 90 (83.3%) were dead within one year (mean 4.4 + 3.5 months). Of the 76 active cancer patients who could be followed and who were still alive beyond one year and up to 46 months (mean 22.0 + 8 months) after the antibody determination, 68 (89.5%) had had antibody levels above 135 micrograms/ml . The relationship of the concent¬ ration of anti-malignin antibody to survival suggested by these data as well as some diagnostic and therapeutic implications are noted. A general transformation antigen is one which is common to the process of malignant transformation rather than to the particular cell type involved. The general antigen therefore differs from eel 1 -speci f ic tumor markers which are related to the products of the ' particular type of cell transformed, as in the case of insulin or thyroid' hormone excesses produced by panc¬ reatic or thyroid- neop1asms , respectively (7). Malignin, a 1 0 , 000 dalto ' n polypeptide from mal ignant giial cells, with a high content of gl utamiQ* acid and aspartic acid and a high ratio of these two aminoacids to histidine, reported in 1975 (1 - 3), and its close structural relatives astrocytin, recognin L . (lymphoma) and recognin M (manrmary carcinoma) (2,4) are members of what appears to be the first chemically and inrmunological 1 y defined family of general transformation antigens. These antigen or anti-malignin antibodies which react with each . -other. h been determined in the cells and sera of patients with a. variety of neoplasms, in induced malignant trans¬ formations in animals and in the cells and supernates of malignant cells growing in tissue culture (5-7,12). Other transformation antigens, not quite as general but broad in representation, are now being identified in other laboratories in experimental cell transformations induced by chemical and viral means (8,9). Over the past seven years, we have examined the possible relationship of malignin and anti-malignin anti¬ body to human cancer states. Previous tumor-associated antigens studied in humans, such as the carcinoembryonic antigens (10) have exhibited varying ' demonstrabi 1 i ty in different types of cancer and low concordance with clinical diagnoses. Perhaps due to the fact that none -SUR£4 has had a constant chemically defined composition or mode of production, the inconstantly released mixtures of antigens rather than a potentially more constant level of specific antibody have had to be measured in serum. Malignin is produced in constant tissue culture of malignant cells, is of known and reproducible composition, and its antibody has been demonstrated to be present in, and isolated from, the serum of patients with cancer ( 6 , 7 ) . The antibody and antigen studies reported here support the apparently ubiquitous distribution of the mal ignant antigen or its very close structural relatives in active cancer of all types examined. METHODS P--t en s._aj d_C£ni. θl_s_ ) Serum Anti -Ma1 i nin_Antibody Studies Cancer patients were chosen by the clinical investigators at each of nine hospitals from various types of cancer in the approximate frequency of their rate of occurrence in their population or of the invest¬ igator's particular interest (see Table 1). Untreated as well as treated cases were accepted. Of the result¬ ing 500 cancer sera studied, 247 (49,4%) were from patien who had clinically and pathologically defined and success fully treated cancer up to 1 5 years earlier and had no clinical or pathological evidence of disease at the time the antibody was determined (fourth control group, below) Of the active cancer group, 76 patients could be followed who were still alive beyond one year and up to 46 months. Four control groups were studied: (1) 59 healthy normals (60 sera); (2) 56 hospital out-patients with some symptom but without definite clinical diagnosis (56 sera); (3) 258 hospital in-patients with definite medical -surgical diagnoses (261 sera); and (4) the 86 cancer patients referred to above who had no evidence of disease at the time of the determination. The medical -surgical diagnose in the third control group included bacterial infections (26 sera), viral infections (28 sera), trauma (8 sera), cardiovascular disorders (30 sera), gastrointestinal and hematopoiet ic disorders (39 sera), thoracic disorders (6 sera), obstetrical and gynaecological disorders (7 sera), genitourinary disorders ( l l sera), endocrine metabolic and arthritic disorders (22 sera), neurologic disorders (62 sera), psychiatric disorders (6 sera), and skin disorders (16 sera). In addition to the above randomly collected sera, selective blind studies have been . ini t iated but not completed on several specific groups: 45 patients with multiple sclerosis (49 sera) and 57 with benign " tumors (74 sera), as well as on 31 blood relatives ('relatives') of canςer patients (31 sera), on people in contact with cancer patients, that is, 54 non- blood relatives and hospital staff ('contacts') (63 sera). 84% of the sera came from the Medical College of Ohio at Toledo. D J i 1 --- π 3M-22_r-l .0-!i£ .-- M----t!l£cl.!. Serum anti-malignin antibody was quantitatively determined by an immunoabsorpt ion method previously described in which the serum antibody is specifically adsorbed on to immobilized malignin ('Target' reagent) in a 2-hour (slow) and a 10-minute (fast) reaction, then released in soluble form and read by optical density at 280 millimicrons as micrograms of antibody protein (11). The values of anti-malignin antibody are expressed as net Target-attaching-gl obul ins ' ('Net TAG') calculated: 2- hour irrmunoadsorpt ion Slow (S) TAG- less the 10-Minute irrmunoadsorpt ion Fast (F) TAG. All values given repres¬ ent Net TAG unless otherwise noted. The Net TAG does not appropriately reflect the antibody elevation when the F- TAG is markedly elevated to between 270 and 1100 micro- grams/ml. In these instances, seen rarely in the four control groups (2 of 464 sera, 0.4%), but in 58 of 247 active cancer sera (23.5%), ' the S-TAG values are also ΓR!3 elevated to above 400 and as much as 12,00 micrograms/ " ml. In " the accompanying figures, to distinguish these cases of extraordinary increase in both forms of anti¬ body, rather than adding the values for the two forms, only the S-TAG has been plotted as open circles. These cases have been examined statistically in two ways, separately, and as part of the clinically determined active cancer group. The antibody determinations were performed blind on coded specimens of sera by laboratory personnel who were in a different center from that in which the specimens were collected, c) Correlation of Cl inj_cal_ .a.nd._LaJ3oratory__Data Corre ' lat ions were made for each patient after completion and recording of bo'ζh clinical and laboratory data separately. ' The error for these correlations in terminal cases is likely to be very small since it involved pathologically confirmed cancer and two reliable dates: the date of the antibody determination and the date of death. For each of 206 of the 247 active cancer cases, in addition to the absence of their names from the tumor registry of deaths, it was possible to verify by contacting each patient or their physician that the patient was still alive at the end of one year. For 41 of these cases, the contact verification either was not possible or possible only to the tenth month. Since most of these 41 cases were from the first two years of the study, when clinically terminal patients were .activel excluded from the study, this is not likely to represent an appreciable error. At most, the. number in the active cancer group would be reduced and the number in the terminal group increased, each by 41, neither of which would significantly influence the conclusions reached except for the value of the mean for the antibody in the terminal group which would be increased. In the statistical comparison of the groups, values of P <0.01 were considered statistically significant. The only [ E comparison of those found not significant under these criteria which approached but did not quite reach the 0.05 level was between the first two control groups (Figure 1 ) . RESULTS Figure 1 of the accompanying drawings shows the concentration of anti-malignin antibody, in micro- .grams/ml serum in individual sera, -in the four control groups and the -active cancer group: that is (1) healthy normals, (2) cancer patients showing no evidence of disease after successful treatment, (3) out-patients (non cancer) with medi cal -surgical symptoms but without define disorders, (4) in-patients (non-cancer) with defined medica1 -sur ical disorders, and. (5) patients with active cancer who lived one year or longer. While the four control groups did not differ from each other at a stat¬ istically significant level, each differed from the activ cancer group at the significant level of P <0.000001. Figure 2A of the drawings shows the concentrat¬ ion of anti-malignin antibody in individual sera of patients with terminal cancer, that is, those who died within one year (mean 4.4 + 3.5 months). The concentrat¬ ion of antibody in this group differs statistically from the active cancer group at a l eve l of P <0.000001. Together with the data shown in Figure 1, it may be seen that 90 out of 108 cancer patients (83.3%) who had anti¬ body levels below 135 micrograms/ml died within one year. In contrast, of the 76 active cancer patients who were longer term survivors and ' who could be followed, 13 to 46 months (mean 22.3 + 8) after the antibody determin- ation, 68 (89.5%) had had elevated antibody levels. Figure 2B of the drawings shows seven examples of the decrease before death observed in individual patients' serum anti-malignin antibody levels when determined serially. Table 1 shows the types of cancer patient studied and the distribution of samples between active disease, terminal disease and no evidence of disease in each type of cancer. The distribution of type of cancer is fairly typical with the exception of an excess number of brain cancer cases which was the initial focus of interest of the study. In the beginning blind study in each of the non- random preselected groups, the antibody level was elevated in the sera of 20.4% of patients with multiple sclerosis, 31.1% of patients with benign tumors, 30.2% of 'contacts' of active cancer patients, and 38.7% of blood relatives of active cancer patients. EXAMPLE_10A TABLE_χ - DISTRIBUTION OF NUMBER OF SERUM ANTI-MALIGNIN ANTIBODY DETERMINATION ACCORDING TO TYPE OF MALIGNANCY AND CLINICAL STATUS TOTAL NUMBER ' CL INICAL STATUS Active No Evidence Type of Malignancy Disease Disease Termina .Carcinoma of: Lung 38 1 1 1 26 Larynx 3 2 1 Breast 67 26 27 14 Uterus 5 1 1 3 Cervix 6 3 3 Ovary 1 1 3 3 5 Vulva 1 1 Colon 37 1 8 3 1 6 Rectum 13 9 2 2 Stomach 2 1 1 Oesophagus 3 1 2 Bile Duct- 1 1 Prostate 13 7 4 2 Bladder 12 5 4 3 Urethra -i ■ 1 Kidney 1 5 6 5 4 Testis . 7 1 5 1 Thyroid 4 4 Pancreas 4 4 Adrenal 1 1 Skin 5 1 3 1 Undifferentiated 1 4 9 5 Hodgkins' Disease 1 4 8 . 3 3 Lymphoma 25 1 5 9 1 Multiple Myeloma 1 5 1 0 2 3 Acute Myelogenous Leukemia 3 2 1 Acute Lymphocytic Leukemia 1 1 Chronic Myelogenous Leukemia 8 7 1 Chronic Lymphocytic Leukemia 8 ; 4 2 2 Fibrosarcoma 1 1 Melanotic Sarcoma 1 5 8 4 1 Osteogenic Sarcoma 6 I 1 4 Rhabdomyosarcoma 4 I 3 Liposarcoma 1 1 Hemangioblastoma 1 - 1 * ' . Histiocytoma 1 • 1 Brain Cancer 1 33 80 2 5 1 Retinoblastoma 1 1 500 247 86 167 Legend for Figure 1: Concentration of anti-malignin antibo EXAMPLE 1 0A in four control groups and in active cancer patients. Solid circlesj Net TAG; open circles-, 5-TAG (F-TAG excess). See Methods for details. Legend for Figure 2: Relation of level of anti-malignin EXAMPLE 1 0A antibody to terminal clinical state. Solid circles, Net TAG; Open circles, S-TAG (F-TAG excess). See Methods for 'details. A. Single blind determination in individual patients. B. Longitudinal blind determination on seven individual cancer patients (1 to 7) whose death (D) occurred 1 to 4 months from date last specimen determined. " DISCUSSION The data obtained in this blind study are consist- ent with the previous evidence that malignin is a general transformation antigen. Thus, rather than being rest¬ ricted to particular cell types, ant i -mal ignin antibody was elevated significantly above normal levels, and malignin was visualized in cells, in patients with a broad variety of active cancer (Tablel and Methods b.) That the antibody was in the normal range in 94.2% of patients who had been successfully treated and at the time of the antibody determination showed no evidence of disease, suggests that an active cancer state is required to maintain elevated antibody levels. In the separation of healthy normal subjects from- active cancer patients by determination of anti-malignin antibody, all healthy normals had values below 135 (mean 59.1 +_ 27.0) micrograms/ml and there were no 'false positives', while in the active cancer group, 92.7% showed elevated values of antibody (mean 273.7 ■+ 156.5 micrograms/ml ) . The healthy normal and the active cancer groups differed at a level of P <0.000001 for the whole active cancer group, as well as for each of the two subgroups shown in Figure 1. As medical ly-i 11 subjects are brought into the comparison (Figure 1), the mean levels of concentration of antibody are seen to shift slightly but not signif¬ icantly upwards. In the out-patient non-cancer group, 94.6% were still in the normal range, and 5.4% were in the elevated range. In -the in-patient, more clearly ill, positively diagnosed (but apparently non-cancer) medical- surgical group, 91.2% were still in the normal range, and 8.8% were in the elevated range. These two control groups were not statistically significantly different from the healthy normal control group but each differed from the active cancer group at a level of P <0.000001. It might be expected that, compared with healthy normals the incidence of cancer would be greater in medically- ill patients and that some of these cancer cases might not yet be clinically di.agnosab1e. How many of these presumptive 'false positives' actually represent occult cancer not yet clinically detected cannot be predicted, but it is relevant to note that six additional 'false positives' were found from one to 19 months later actually to have clinically and pathologically proven cancer . The data in the preselected groups, although blind were not randomly collected as were those in Figures 1 and 2 and therefore cannot be pooled with them. Each of these preselected groups is considered too small to form conclusions because of heterogeneity of each and the complexity of the implications raised by the data, but they are included as preliminary data for the sake o completeness, There is a possibility that, in the destructive and immune reactions in the nervous system in multiple sclerosis, a higher false positive rate may occur. Some of this may represent misdiagnosed central nervous system malignancy. Sera from patients with benign tumors might be expected to show a higher false positive rate consistent with the borderline area in c 1 i nico-pathological diagnosis between benign and malignant growths. Anti-malignin antibody levels and the demonstration of malignin in cells may in the future help to clarify the definition in this group. The observation of a higher incidence of elevated anti- malignin antibody in contacts of active cancer patients (compared .wi th healthy normals P <0.001) is in agree¬ ment with several previously published studies on other tumor indexes demonstrating the same curious phenomenon (14 clinical studies and one laboratory study cited in reference 14). Whether this represents some form of immunization against a transmi ttable agent, either the malignin antigen itself or a substance which induces transformation and thus the appearance of the antigen, needs more work to clarify. Finally, the greatest incidence of antibody elevation in a 'non-cancer' group is observed in the blood relatives of active cancer patients. Whether this represents a response to actual cell transformation, a genetically determined high level of production of the antibody for immunosurvei 1 Iance, or the same phenomenon as that observed in the 'contacts' group is unknown. Since the 'celatives' are statist¬ ically different from the 'healthy normal' control group at a level of P <0.000001, some explanation will have to be sought and certainly much larger groups will have to be examined. The utility of the malignin antigen and the anti¬ body for general screening of populations for cancer is suggested by the low 'false positive' rates shown in Figure 1 in the healthy normal and out-patient control groups. The results of the present studies also indicate within the limitations of all laboratory procedures, that both the determination in cells of malignin and in serum of its antibody may be useful in helping to recognise the presence of malignant states in. individual s in whom cance is suspected. In addition, the clinical follow-up of ' individual patients over months and years has permitted the comparison of clinical outcome with antibody levels which were obtained on blind coded serum specimens. The correlation observed suggests that the anti-malignin antibody level may be related to survival in that the elevated values during active disease were associated with longer survival and low levels during active disease with early death. ' After successful treatment, however, the presence of normal (low) antibody levels may be. an aid in determining whether an active cancer state has been replaced by one in which there is 'no evidence of disease'. Once again, the laboratory value can have relevance only in relation to the clinical status, and it usually should not be difficult to separate the clinically healthy from the clinically terminal patient, both of whom have low levels of anti¬ body, but for different reasons ' . The significance of the correlation of lower levels of anti-malignin antibody with terminal illness , shown in Figures 2A and 2B is not known. Since as seen in Figure 2B, -the drop in antibody can occur abruptly, in as little as one month before death, it is not known how many of the elevated values shown in Figure 2A were followed by a similar drop prior to death. The drop may therefore be even more common than is observed in the single determinations. The phenomenon is in accord with previous demonstrations by others of the general decrease in irrmunocompetence observed to signal oncoming death in the case of both human and animal cancer (15), and may simply represent a secondary consequence of the terminal state. However, since anti-malignin antibody is specific for a cancer cell antigen, localized prefer- entially in malignant cells £n vi££θ and _rι vivo, and » has been shown to be cytotoxic to malignant cells _n vj_ £θ ( 7 ) , the drop in antibody might be more central to the cancer process and be to the detriment of the patient In addition, earlier data (6) showed antimalignin anti- body in human cancer sera to be largely 'disarmed', with its Fc portion cleaved from the Fab fragments, which would result in loss of cytotoxicity. This process might reflect one form of the cancer cell's defence against the antibody. The low levels of ant ibody "observe here prior to death may be evidence of a second form of the cancer cell 's defence, -the result of an ' increas ing blockade of antibody production or release due to antigen excess as the tumor proliferates. That malignin is not an 'onco-fetal' antigen is supported by the absence of malignin from fetal tissues. Malignin appears to be. much older phylogenetical ly than those states commonly thought of as being recapitulated during fetal development; its only structural relatives, by computer search (16), are the ferredoxins of plants, H≤i-i- llϋ S -LH≤^ an< ^ alfalfa, the acyl carrier protein of E. coli, and cytochrome b5. These four share the property of being anaerobic enzymes, the ferredoxins being the most electro-negative oxidation-reduction enzymes in nature. Warburg observed the anaerobic advantage of malignant cells but was unable to account for this property in the activity of the then known anaerobic enzymes (17). The possibility that malignin is a cleaved derivative of such an anaerobic enzyme system, that this system is common to all malignancies regardless of cell type, and that this system imparts an unique anaerobic advantage to cancer cells, would be consistent with the demonstrated increase in the yield of malignin with increasing malignancy of cell growth (1,2), the ubiquity of distribution of the antigen, the cytotoxicity of the antibody and the antibody failure in the terminal state. Now that purified human anti- malignin antibody is available (6,7), and monoclonal anti malignin antibodies are available, the therapeutic uses of the antibody acting alone or as a carrier for anti-cancer drugs can be further systematically examined. REFERENCES_FOR_EXAMPLE_J_0A 1. Bogoch, S. Brain glycoproteins and recognition function: Recognins and cancer. Pages 555 -556 . In Volk, B.W. and Schneck, L. (eds), Current ∑L--L-l-i _i-l_^E-li!l£° Ei^-2 _ a _l-_^ i£_i-_Qi-i .L- -l.» Plenum Press, New York, 1976. 2. Bogoch, S. Astrocytin and malignin; Two polypeptide fragments (recognins) related to brain tumor. • Nai^_Cance£_ins_£_ : __Mon. 46: 133-137, 1977. 3. Bogoch, S. The detection of malignant gliomas in brain by the quantitative production _n vj_££o ' of TAG (target-attaching globulins) from human serum. Pp. 358-361. In Bogoch, S. (ed) Bj_o_ og cal Diagnosis of Brain Disorders . Spectru -Wi ley Press, New York, 1974. • 4. Bogoch, S. and Bogoch, E.S. Production of two recognins related to malignin: Recognin M from mammary MCF-7 carcinoma cells and recognin L from P.,3 lymphoma cells. Neurochemic 1 Res . 4: 465-472, " 1979. 5. Bogoch, S., Bogoch, E.S. Fager, C. , Goldensohn, E. , Harris, 3.H., Hickok, D.F. Lowden, J.A. Lux, W.E., Ransohoff, 3., and Walker, M.D. Elevated anti-malignin antibody in the serum of cancer patients; A mul ti -hospi tal blind study. N Hr_£ £&y_ 29: 584, 1979. 6. Bogoch, S., and Bogoch, E.S. Disarmed anti- malignin antibody in human cancer. Lance£, 1, 987, 1979. 7. Bogoch, S. and Bogoch, E.S. Tumor markers: Malignin and related recognins associated with malignancy rather than with cell type. In Battistin, L., Hashim, G. , and Lajtha, A. (eds) Neurochemi stry and Clinical Neurology, pp. 407- 424. Alan R. Liss,- Inc., New York, 1980. 8. . Rigby, P. The transforming genes of SV40 and polyoma viruses. N ture 282: 781-784, 1979. 9. Langan, T. Malignant transformation and protein phosphorylat ion. Nature 286: 329-330, 1980. 10. Krupey, 3., Gold, P. and Freedman, S.O. Physico- chemical studies of the carcinoembryonic antigens of the human digestive system. 3.__Expt 1 ♦ Med. 128: 387-398, 1968-. ' 11. Bogoch, S. and Bogoch, E.S. Quantitative . -determination of anti-malignin antibody. In Rosenberg, S.A. (ed) Serol gic_Anaj_y S of_Human Cancer Antigens , pp. 693-696. Academic Press, Inc. , New York 1980. 12. Harris, 3.H., Gohara, A., Redmond, F. , Bogoch, S. and Bogoch, E.S. I munof 1uorescent and serologic studies with anti-malignin antibody. In Rosenberg, S.A. (ed) Serologic Analysis of Human Cancer Antigens, pp. 571-582. Academic Press, Inc., New York, 1980. 13. Meek, R.A., Ingram, M. , Meek, 3.. 1 . , McCul lough, 3.L., Wu, M-C, and Yunis, A.A. Establishment and Cell Cycle Kinetics of a Human Squamous Cell Carcinoma in Nude Mice and in Vitro. Cancer_ Res . 4: 1076-1085, 1981. 14. Editorial, The Cancer Connection. Lancet 1: 635-636, 1977. 15. Hersh, E.M. , Gutterman, 3.U., Mavligit, G.M. , Mountain, C.W. , McBride, C ., Burgess, M.A. , Lurie, P.M., Zelen, M. , Takita, H. and Vincent, R.G. Itrmunocompetence, Immunodeficiency and Prognosis in Cancer . Ann. New..York _Acad. , 5ci . 276: 386-406, 1976. 16. Dayhoff, M.O. (ed) Atl_as _of .Protein ...Sequence .and ----- H-Ξ . H££ . • National Biomedical Research Found¬ ation, Silver Springs, Md. , 1972. 17. Warburg, O. , Gaweh, K. , Geissler, A.W. , Schroder, W. , Gewitz, H.S. and Volker, W. Arch_. _Bjochem. B oRhls^ 78: 573, 1958. EXA PLE_π Diagnosis of Tumor Cells by Irmnunof1uorescence. The compounds Anti -Astrocyt in, Anti -Mai ignin, and S-TAG have been shown to attach preferentially to'tu or cells. This specificity permits use of these compounds to diagnose tumor cells ,in histology sections by conjug- ating dyes or radioactive substances to Anti-Astro- cytin, Ant i -Mai igni n , or S-TAG. Standard labelling techniques may then be used. A procedure using S-TAG is as fol lows . One procedure which has been found satisfactory is a modified St. Marie procedure. Human brain tumor specimens are frozen and 5 micron thick sections cut. These are stored in a moist container at minus 70°C for 4 to 8 weeks before staining. The conjugate may be a standard anti-serum such as goat anti-rabbit conjugate. The conjugate is labelled by techniques known in the art with fluorescein or other labelling substance. Fl uoresce in- 1 abe11 ed goat anti-rabbit conjugate as commercial 1 y available may be used. The fluorescent technique used was a standard one in which a 1:200 to 1:400 solution of TAG is incubated for 30 minutes or more on the tumor section, followed by washes to remove unattached TAG. Three washes with phosphate buffered saline has been found satisfactory. Conjugate incubation with f 1 uoresce i n- label I ed conjugate followed by washes is then performed, followed by microscopic inspection. Normal ceils and their processes fail to stain both in tumor sections and in control sections of normal non-tumor brain. Fluorescence is brightly present in tumor glial cells and their processes. EXAMPLE_]_1A Dg ggt ion q1_N° -- . --- gift. „ . , M a RP.an Ce1 l_ s_ ith Fluorescent The uses of TAG products coupled with a signal emitter such as a dye or a radioactive label to detect cancer cells is described, for example, at pages 12-18 and Example 11 herein. In this Example 11A, the detection of non-brain malignant cells is described. As described in Example 10 utilizing human serum in the determination of TAG, after the anti-malignin antibody was bound to the .irrmobi 1 i zed antigen and non- bound serum proteins were washed away, the antibody was cloven from the binding with 0.25 M acetic acid at 37°C for 2 hours and the TARGET reagent was separated out by centrifuging. The TAG antibody solution was quantitated by means of its absorption at 280 mμ. The TAG solutions were stored at -20°C, then thawed and combined, brought to pH 7 by titration with 6N NaOH, dialysed against phosphate buffered saline of pH 7, filtered and concentrated on Millipore Pellicon 1000 membranes, centrifuged to clear insoluble protein and the immune globulin complexes, concentrated and freed of iπmunological ly non-active compounds by Cellex D and Blue Sepharose C16B (Pharmacia) chromatography. This human anti-malignin antibody reacts with anti-human ganrma globulin in Ouchterlony double diffusion. When TAG is used with fluorscein conjugated to anti-human ga ma globulin in standard double layer Coons irrmuno- fluoresence, it stains malignant glia, breast carcinoma, ovarian carcinoma, adenocarcinoma of the colon, and other types of cancer cells in postoperative and biopsy tissue sections, as well as inhuman sputum, bronchial washings, pleural effusion fluid, gastric aspirate and bladder urine. The concentration of protein in TAG which yields clear fluorescence when controls are negative, is 1 to 10 μg per section. The production of a "purified" TAG was undertaken by reacting the sera from patients with a variety of - cancers with bromoacetyIeel 1u1ose - MALIGNIN by methods ear 1 i er .descr ibed (Example 8). The. antibody bound in this reaction was cleaved with 0.25 M acetic acid, quantified by measurement at O.D. 280 using a conversion factor of 1.46 for gamma globulin frozen and stored at -20°C. This antibody was found to contain immunoglobul i as determined by anti-human garmπa-globu1 in antiserum specific for ganrma chains (BioRad Laboratories, Inc.) an wi th ant i-FAB and anti-Fc -fragments (Miles Laboratories). It also reacts with rabbit anti-human albumin (BioRad Laborator ies ) . It was found that, whereas 10 to 50 micrograms of protein TAG are required to produce specific imrnuno- fluorescent staining of cells which contain Malignin, only 1 to 10 micrograms of purified protein TAG are required for this specific staining in all sections, arid in a few, less than one microgram has been found to suff ice. It was found that the most active preparation of purified TAG is that which is eluted with the highest ionic strength eiution, i.e., from 0 . 1 5 M to . 5 M. Any method of -production which uses this fact is useful; three preferred methods are given below. Met . h-.od_I " Fractionat ion of TAG chromatography with DEAE cellulose (Cellex D, BioRad Laboratories) was first employed using step-wise eiution with increasing ionic strength and decreasing pH, and the same sequence of eluants as that given in Example I for the production of Crude Astrocyt in-Precursor-Contain ing Fraction. Good separation was obtained of the bulk of the protein into three fractions, Peak I obtained with Solution 1 (see Example 1) and Peak II obtained with Solution 1 (see Example 1) and Peak II obtained with Solution 6 and Solution 7. Ouchterlony double diffusion showed the TAG in Peak I still to contain appreciable protein with albumin mobility, and while Peak II contained most of the albumin, appreciable I G could be detected. Re- ch . romatography of Pea . I gave a progressively pure IgG until, after the seventh chromatography, essentially no albumin (less than 3%) could be detected by Ouchterlon gel diffusion in which 5 to 10 micrograms of human albumi were detectable with rabbit anti-human albumin. The IgG so obtained was prone to denaturing and loss of irmnuno- logical reactivity after a few days' standing at 0-5°C. Method II - A second f ractionat ion of TAG was made with chromatography on Sepharose CL-6B (Pharmacia, Inc.) starting with low molajity buffer (0.0005 M phosphate) and proceeding in two steps of 0.15 M and 1.5 M to elute the balance of the protein. As with the Cellex D, one passage was found to be inadequate to separate, and recycling slowly improved the product. Once again, the most active fraction vis-a-vis anti-malignin antibody was in the 1.5 M fraction. Method 11 I - Chromatography with Sepharose CL-6B next to the glass fritted disc and Cellex D layered above the Sepharose proved to be the most satisfactory method. The graphical representation in Fig.. 1 shows the fractions obtained on chromatography of TAG utilizing Method III. After the first eluate of 200 mis., 50 ml. or smaller sub-fractions were collected. The protein content of each eluate was determined by the optical density at 280 mμ with an uniform factor of 1.^46 based on ganrma globulin used to convert to micrograms for calculating recoveries. The absolute amount of protein . requires correction in those fractions in which there is appreciable albumin. The points at which the stepwise solvent changes were made are indicated by arrows. The subfractions are designated by Roman numerals I to VIII, inclus ive. - The solvents corresponding to letters A - F at the arrows were as follows: A - 0.01 M TRI5 (pH 7.2) B - 0.05 M TRIS with.0.1 M NaCi (pH 7.2) C - PBS, 0.11 M NaCl (pH 7.2) D - PBS, 0.165 M NaCl (pH 7.2) E - PBS, 0.33 M NaCl (pH 7.2) F - 0.05 M TRIS, 1.5 M NaCl (pH 7.2) In the following Table are shown the recoveries fro each fraction, a semi-quanti tative determination i-n each of the gamma-globulin and albumin in each, as well as the activity of each fraction in the iπmuno 1uorescent OMPI staining of cancer cells (the plus sign indicates reactio zero no reaction and plus/minus reaction in some cases). TABLE Fraction I ' II III IV . V VI VII VIII Recovered μg 2,877 1,140 2,35L 2,942 1,808 2,230 2,125 7,477 % 12.5 5.0 10.2 12.8 7.9 9.7 9.3 32.6 Im ' munodif fusion Against: Anti-human IgG specific * for gamma chains +++ ++ ++ + o + ++ +++ Anti-human ++ +++ +++ +++ +++ albumin Anti-Fab ++ ++ ++ ++ Anti-Fc ++ ++ ++ Immunofluorescence + + + + + +++ Photographs were prepared showing the line of reaction between anti-human gamma-globulin specific for garrma chains for " each of Fractions I and VIII from above. Photographs were taken showing the use of TAG (Fraction VIII from above) to stain non-brain malignant cells, i.e., a stain of bronchogenic carcinoma cells in the bronchial washings of a patient and a stain of lymphoma cells in the pleural fluid of a patient. Non- cancer .cells do not fluoresce. The TAG (1 to 10 μg in 0.1 ml phosphate buffered saline (PBS)) is applied to the surface of packed cells-on a glass slide, incubated for 30.minutes, washed -three times with PBS and then layered with f luorescein-conjugated anti-human IgG diluted until non-malignant control tissues give essentially no fluorescence. The cells are visualized with a Zeiss fluorescent microscope using a tungsten lamp and filters BG 23, BG 12 and 500. EXAMPLE_12 Blind Study_of TAG_5peci fj i ty_ _in_ Irrmunof1.uqrescence The presence of malignin was sought in cells collected from cancer patients and controls. Specimens were collected by thoracocentesi s, paracentesis, bronchial or tracheal washings, sputum and pericardial effusion, from patients with lung, breast, prostatic, colon and undi fferentiated cancers, as well as from non-cancer controls including patients with emphysema, heavy smoking and epilepsy; and sputum from a former cancer patient with no evidence of disease for two years following successful treatment. Cells were concentrated by centrifuging. The following Table shows the correlation of the presence or absence of malignin in cells as determined blind by immunof1uorescent staining with anti-malignin antibody (TAG), and the cl inical -pathological diagnosis. The TAG stain result was correct in 20/22 specimens (91%) Standard Papanicolaou stain examinations performed blind on duplicates of these specimens by other pathologists were correct in 17/22 specimens (77%). CELL MALIGNIN: IMVIUNOFLUORESCENCE TAG RESU cancer non- -cancer total Ci inical - Cancer 14 2 16 Pathological Diagnosi s Non-cancer 0 6 6 Total 14 8 22 In addition to the positive stain for malignin in cells from breast, ovarian and bronchogenic carcinoma and astrocytomas , cells grown in tissue culture from human squamous cell carcinoma of the vulva, and from five different types of human lymphoma, as well as leukaemic cells in both acute and chronic leukaemia blood have demonstrated positive staining. Malignin was visualized and photographed in a variety of human cancer cells by anti-m lignin antibody double-layer immunof 1uorescence. The second layer fluorescein- iabelled ant i -ant ibody was diluted in control experi¬ ments to as much as 1:1,600 until non-specific fluor¬ escence was completely eliminated in the absence of the first layer anti-malignin antibody. Under these conditions, anti-malignin antibody was active at one nanogram antibody protein per cancer cell in producing the specific immunof 1 uorescence seen and photographed in: A- bronchogenic carcinoma cells, from bronchial washings; B- lymphocytic leukaemia cell, from blood; C- ovarian carcinoma cells, at surgery; D- squamous cell carcinoma (2 cells), grown in tissue culture; E- astro- cytoma, anaplastic, at surgery. EXAMPLE__3 -----li££i £-l_£i_Can e£_Cell^_w__^ TAG In this Example, the feasibility of attaching a radioactive label to TAG is demonstrated. Second, the injection into animals of this radio- iabe11ed TAG has been accomplished and shown to be safe and effective. Third, the radio-1abel led TAG localized preferentially in the cancer tissue when compared with normal tissues, thus indicating that the specificity previously demon¬ strated i_n _____ of the preference for cancer cells which is conveyed by the use of specific ant i -Mai Ign in TAG products is confirmed __ ____ . The_Labej__ng_qf_TAG_w__h_9j^ _______________________ 1. -Two preparations of TAG were used, here designated TAG-1 and TAG-2. TAG-I and TAG-2 (concentration of each: 0.4 mg/0.5 ml) were added to separate sterile evacuated vials. 2. To each vial was added 0.1 ml of a stannous chloride solution (10 mg 5nCl_. 2 H_0 in 100 ml of 0.01 -N Hc.l ) . The vials were mixed for 3 - 4 minutes . 3. 0.1 ml. (6mCi) of lc-pertechnetate (sodium salt) was added and mixed 2 - 3 minutes. Procedure ,, .fo _Det_eπrni_ni_ng Labelling Efficiency Samples " of the "^Tc-TAG-l and π c-TAG-2 were tested for labelling efficiency by descending paper chromatography using Watman No. 1 paper with 85% methano as the solvent. A similar study was done with Sodium Pertechnetate- ^Tc which acted as a control. After 2 hours, the papers were removed from the chromatography tank and divided in two sections: (1) 1 cm about the origin; (2) the remaining paper up to the solvent front. Each section was then counted in a gamma well scintillation counter and its content of radio¬ activity determined (cpm). Approximately 50 labda were plated on each paper strip. P£qc q^u£ _£££_An£_ ei ^Ar^£_^qα^y__Reac£_£n A portion of the labelled solution was also plated on an Ouchterlony gel plate to determine its ability to react with malignin in the antigen-antibody reaction. After a 3-hour period, the resulting sharp reactive lines were removed from the gel and their content of radio-activity was measured. An equal portion of the gel not involved in the reaction was also removed and its content of radioactivity was also measured as " background. Results . . * ______________ _____ IABLE___-_Labe l __ng_Eif__c i enc^ and 99m Tc -TAG- 2 COMPOUND SiϊE_ON_PAPER CPM % CHEMICAL SPECIE NaTcO ψ -" m Tc origin 4.94 x 10- 7.33% reduced Tc0 ώ - 4 NaTcO .- 99m Tc solvent front 6.25. x 10' 92.67% TcO - TAG-1 origin 4.35 x 10 ( 98.47% TAG- 99m Tc TAG-1 ssoollvveenntt ffrroonntt 6.76 x 10 ' 1.53% TcO^- TAG-2 origin 1.96 x 10 6 98.01% TAG- 99m Tc TAG-2 solvent front 3.98 x 10^ 1.99% TcO,. CEL_AREA C2 'NTS_PER_M_N_ % TAG- 2 lirie 1.99 x IO 6 92.04% Background gel 1.72 x 10^ 7.96% Conc l u_s_on^ The following conclusions were reached relative to the quality control tests emp1oyed : 1. ιc-pertechnetate was reduced by stannous chlori to a more reactive oxidation state (+4+5). 2. The reduced pertechnetate labelled both the TAG- 1 and TAG-2 preparations. 3. The *π " c-TAG-2 was tested for its ability to retai its activity and was found to retain its ability t react irrmuno1ogica11 y. The_U∑e_qf_Rad_o^Labe__ed_TA^ _-iil Wistar rats were injected intracerebra11y with C6 glioma tumor cells which had had previous passages in rats and in tissue culture. The rats were observed for the first signs of growing tumor, such as weakness, tremor or unsteadiness. These symptoms first appear seve to ten days from injection, and with fast growing tumors result in death within three to four days in many animals and one week in all. As soon as symptoms appeared, the animals were injected with labelled TAG intravenously in the tail vein, then the -animals were anaesthetized at varying times, the brain was removed, the tumor was dissected free of normal brain, and the radioactivities of the dissected specimens were compared with one another Prel iminary ^g-TAG experiment c ... _ Counts/gm/min. Sacrifice Tumor ia Animal (hr. post injection) wt.,mg. Tumor - Normal Brain A 1.25 1.9 149,100 13,400 B -5.30 6.0 16,200 6,600 C 7.21 23.0 53,000 5,800 D 24.10 29.0 66,700 7,500 Tumor and normal brain specimens were counted over¬ night in the gamma-well counter. All samples and stand¬ ards were decay corrected for convenience to the mid- count of the first sample in the, sequence. Conc_l_u__qrι The preferential localization of radioactivity in tumor as compared with normal tissue is demonstrated abov EXAMPLE_14 PRODUCTION OF MONOCLONAL ANTI-MALIGNIN ANTIBODIES, MAMA-S, MAMA-F, AND MAMA-FS, AND THEIR RESPECTIVE NOVEL PRODUCER CELLS A myeloma cell line (P3x63-Ag-8) was cultured in Dulbecco's minimum essential medium supplemented with 10% foetal bovine serum (D ) ( ,), in a humidified incubator at 37°C and 5% C0 2 . Inbred female BALB/c3 mice (8 weeks of age) (3ackso Laboratory, Bar Harbor, Maine) were immunized intra-- per i toneal ly, 4 times at weekly intervals with 1 mg. Malignin emulsified in complete Freund's adjuvant (Difco). Sera of the immunized mice were tested for the presence of anti-malignin antibody and antibody positive mice were further boosted 4 days prior to cell fusion. s Immune spleen cells (10 ) were fused with the myelo cell (10 ) using polyethylene glycol (PEG, 1000, 3.T. Backer) as the fusion inducing agent as described by Galf et al (Nature 266, 550 -55-2 , ' 1977). The PEG treated cell mix was seeded into 96 wells of microtiter plate (Costar 3596) in D, Q supplemented with hypoxanthine, aminop- terin, and thymidine (D ]0 HAT) (Li ttlef ϊeld, 3.W., Science 145: 709, 1964). About one-half of D ' HAT was replaced twice weekly for two weeks. The spleen cells did not survive _n ___£__, while the infused myeloma cells were killed in D._ HAT. Only the hybrid cells remained actively growing after 0 days under the selective conditions. After two weeks in D. n HAT, th hybrid cells were fed for another week with medium which was the same as D. n HAT except for the omission of aminopterin (D ]n HT) then with D " . Whenever the wells were about 80% covered by hybrid cells, supernatants were aspirated for anti-malignin antibody assay.. Cells from the antibody-producing wells were cloned in soft agarose by modifications of the method described by Cotton e_ aj_ (Eur. 3. Irrmunol. 3, 135-140, 1973). Briefly, an equal volume of warm 0.8% agarose (Seaplaque, Marine Colloid Inc.) and double strength D. 0 were mixed and plated 2 ml to a 60 mm dish and chilled at 4°C for 1 5 minutes as base, layer. One thousand cells in the same medium were overlayered on the base layer and chilled, then incubated under the same conditions as regular cell cultures. The anti-malignin antibody positive clones were further grown as ascitic tumors in BALB/c3 mice. ™LE___EXA^kE_i! Quantity of Antibody (μg/ml extracellular f luid) for_Each ! -!-Hii- £-Y-_E °£_H£iE&_C ££ (F= fast react.ing,_ in 10 minutes; 5= slow react ' i-ng.^_ir-L-2„ hours; FS= both types of antibody produced Method as in Example 10) Extra-Cellular Months After Fluid Manufacture of PRODUCER CELLS MAMA-F MAMA-S MAMA-F/S Cell 38 32 22 27 21 25/21 Supernate 1 mo. - 67 32 38 37 27 21/21 19 42 27 53 21 21/19 27 30 55 62 25 25/23 25 21 27 32 23 29/27 26 '29 37 26 44 48/45 52 23 29 34 33 2/18 30 Cell Supernate 3 mo. 51 16 41/34 41/39 136 15 36/36 29/23 73 16 47/41 39/37 44 29 39/33 30/27 32 62/58 22/22 30 101/110 15/18 23 30/29 18/18 Cell Supernate 4 mo. 19 30 18 18 30/34 32/29 32 30 26 23 15/16 21/23 30 27 25 27 27/26 23/21 27 29 30 30 29 Cell 126 140 34 88 47/97 49/82 Supernate 5 mo. 178 393 248 69 22/27 74/178 162 296 89 114 26/30 83/149 92 123 56/127 94/232 308/82112/79 62/107 56/169 164/390178/164 249/301 Mouse 660/1,070 Ascites 8 mo. 780/670 Fluid The above Table shows the quantities of monoclonal anti-malignin antibody produced by each antibody producin clone, in micrograms of protein per mi of extracellular fluid. The yields of antibody are seen to be good for the first four months of propagation of the clones, and to have increased by the fifth month of propagation. The cells continued to grow well through the eighth month and to successfully grow when transferred i nt raper i toneal 1 y to the mouse, where the yield of antibody again increased as expected to as much as 1 mg . of MAMA-5 per ml of ascites fluid. The cells also grew successfully on soft agar and were- frozen and stored in liquid nitrogen and grown again after thawing. Aliquots of each clone were frozen in liquid nitrogen for permanent storage and regrowth at later dates. The monoclonal antibody in each case was quantified as protein by optical density at 280 millimicrons, was non-dialysab1e, and migrated on SDS-polyacry1amide gel e I ectrophores i s predominantly as garrma chain inrmuno- 1obu1 ins . With progressive recloning, each specific mono¬ clonal antibody-producing ccell was concentrated. Thus, recloning of MAMA-B Producer Cells yielded four out of six colonies which were MAMA-B .Producers , and recloning of MAMA-A Producer Cells yielded three out of four colonies which were MAMA-A Producers. Each of the three types of antibody stained a wide range of malignant cells by immunof 1uorescence in approximately the same concentration range as previously observed with purified TAG products. That is, one nanogram of antibody protein stained one cancer cell. Photographs were taken of specific immunof 1uorescent staining obtained with human leukaemic blood, both acute and chronic, six culture lines of leukaemia cells (3Y, KARPAS, CEM, RA I , HL60 and K562), and three human lymphomas. 5taining was obtained with MAMA-F, MAMA-S and MAMA-FS. Second layer staining with fluorescent labels, both fluorescein and rhodamine, at concentrations as low as 1:1,600 was observed and recorded. These very low concentrations of the second layer permitted dilution until background non-specific staining was eliminated, and, at those concentrations of second layer (FITC or rhodamine), highly specific staining was obtained with MAMA-F, MAMA-S and MAMA-FS. EXAMPLE 15 •_-2---MO STRATI^_BY_OT "MALIGNIN FLUORESCENT INDEX" WITH MONOCLONAL ANTI- MALI_GNI_N_ANTI_BODχES_F_&_S Using the two monoclonal anti-malignin antibodies, MAMA-F and MAMA-S, in several concentrations, time of incubation, washing or no washing, different concentrations and times of incubation of fluorescein i sothiocyanate anti-mouse antibody (FITC), and other specifications as to the method of preparing blood and/or white cells, in both normal and cancer sera ( leukaemias , . lymphomas ) , in a study of how these antibodies may best be used with flow cytometry instruments, the following conclusions and preferred examples are discussed. 1. By quantitating the actual number of cells fluorescing per 100 cells counted and correcting for the ceils fluorescing without MAMA but with the FITC antibody alone, an absolute number is obtained which represents the true or specific fluorscence due to MAMA. Thus, Malignin Florescent Index = (Number of Cells fluorescing with MAMA plus FITC) less (Number of cells fluorescing with FITC alone) 2. The Malignin Florescent Index is a rapid diag¬ nostic test for malignant cells in fluid suspension, which distinguishes normal from malignant cells regardless of the cell type (malignin is a general transformation antigen which relates to the process of malignant trans¬ formation rather than the cell type). 3. Examples from the data obtained: Cells fluorescing/100 1 (corrected) Date + MAMA Time, Washed Specimen Used mih. Region Region Region Specific P ] FITC 2 3 4 Total * * sce 4/29/81 min. Ind Normal 0 20 0 30 4.1 13.3 36.5 Normal MAMA-F 20 0 30 3.5 12.3 31.7 specific -0.6 - .0 - 4.8 - 6.4 Leukaemia 0 20 0 30 11.8 7.8 3.0 (L) Leukaemia MAMA-F 20 0 30 15.4 7.8 3.2 (L)(Acute) specific + 3.6 0 + 0.2 + 3.8 1 Normal O vs. 60 10 - 5.4 - 0.2 1.6 4.0 MAMA-S Leukaemia O vs. 60 10 + 0.8 + 0.3 - 0.2 + 1.3 + 5 (L) MAMA-S Leukaemia (VH) O vs. 60 0 - 2.7 -14.1 + 0.5 -16.3 -12. Chronic MAMA-S Lymphoma O vs. 60 10 + 0.1 + 0.4 +11.8 +12.3 +16. (SIO) MAMA-S Lymphoma O vs. 60 10 - 0.5 - 0.3 + 6.1 + 5.3 + 9. (515) MAMA-S Lymphoma O vs. 60 0 - 0.5 - 0.2 + 4.5 + 3.8 + 7. (S16) MAMA-S .- EXAMPLE 16 ___- I-1Δ1______^^ CULTURE Standard tests for determining cytotoxicity may be used. Generally, the number of cells in a fixed -countin chamber, usually arranged to contain about 100 live cells, is counted. These cells are then treated with the agent being tested and the number of cells which are still alive is counted. In a standard test cytotoxicity of S-TAG Solution obtained in accordance with the methods of Example 9 against cells in tissue culture derived from a patient with a gl ioblastoma Grade III-IV, well characterized as of glial original, S-TAG produced death of all cells in the counting chamber even when in high dilution of 1:00 and 1:1000, representing as little as 0.2 and 0.02 μg of S-TAG per ml. of so.lution. Similar results are obtained with high dilutions of Anti-Astrocytin and Anti -Mai i nin. Both the specificity exhibited in Examples 11, 11 12, 13, 14 and 15 and the cytotoxicity demonstrated in t Example and Example 17 are highly relevant to the therap eutic possibilities of- Ant i -As.trocytin, Anti -Mai ignin an 5-TAG for malignant tumors in man. The practical diag¬ nostic potential of both of these phenomena for tumor tissue removed at operation but requiring diagnosis by histology is already demonstrated herein. EXAMPLE_17 DEMONSTRATION OF CYTOTOXICITY OF A MIXTURE OF MONOCLONAL ANTI-MALIGNIN ANTIBODIES MAMA-F AND MAMA-5 Whereas either MAMA-F or MAMA-S alone does not produce appreciable cytotoxicity with malignant cells, when these two monoclonal antibodies are mixed, the mixture is actively cytotoxic to malignant cells. In addition, the product MAMA-FS is cytotoxic. All three preparations are cytotoxic in approximately the same concentrat ion-s as previously observed for Anti-Astrocyti Anti -Mai ignin and 5-TAG products (Example 16). Approx- imateiy one nanogram of antibody per cell is cytotoxic, resulting in lysis of the cell. Cytotoxicity also was observed and recorded on both the Coulter cytof 1uorograph and on the Ortho cyto- fluorograph, each of which permits absolute counfs of viable cells with time. Destruction of viable malig¬ nant ceils (carcinoma c£ the pancreas, leukaemic and lymphoma) was observed over a period of 1 5 to 60 minutes. ' Those malignant cells which were identi-fied by light scatter and specifically by fluorescence were destroyed by either the mixture of MAMA-F and MAMA-S or by MAMA-FS. The killing of cancer cells is, by definition, a therapeutic process, and 'the products which produce this killing, are by definition, therap¬ eutic products . As disclosed above, the various PRODUCER cells have had their desoxyr ibonucleic acid specifically programmed by MALIGNIN to produce the ant i -MALIGNIN antibody. These include monoclonal anti-Mai ignin anti¬ body FAST producer cell, SLOW producer ceil and FAST and SLOW producer cell. It will be appreciated that this invention covers both the desoxyr ibonucleic acids and the ribonucleic acids of all such PRODUCER cells.

Claims: - 72 CLA IMS : 1. A process for producing monoclonal anti-cancer RECOGNIN whereby the cancer RECOGNIN, or a purified fraction thereof, which when injected into an organism has the ability to induce or to cause cells of said organism to produce specific anti-caner RECOGNIN, which forms a single 1 ine precipi tate with its specific anti¬ body in quantitative precipitin tests and Ouchterlony gel diffusion tests, is soluble in water and aqueous solutions having a spectrophotometr ic absorption peak wave-length of 280 mμ and a molecular weight of from 3,000 to 25,000, and further has an aminoacid residue composition with high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic aςids to histidine, characterized in that such anti- cancer RECOGNIN producing cells are subsequently treated to make them sel f-perpetuating and identified by their production of ant-cancer RECOGNIN, said cancer RECOGNIN comprising a product, derived from cancerous tumor tissu or eel 1 s . 2. The process according to claim 1, wherein said organism is a mouse or other mammal. 3. The process according to claim 1, wherein said induced ceils are spleen cells. 4. " - Tire process according to claim 1, wherein said treatment of the induced cells is a process of hybrid¬ ization whereby said induced cell is hybridized with any sel f-perpetuat ing cell to produce an unique hybridized sel f-perpetuating cell. 5. The process according to claim I, wherein treatment of the induced * cell involves their hybridiz¬ ation with myeloma cells. OMPI 6. The process according to claim 1, wherein said sel f-perpetuat ing cells are selected by cloning and testing each clone thus produced for its ability to produce the specific monoclonal ant-cancer RECOGNIN. 7. The process according to claim 1, wherein said ant i-RECOGNIN antibody is an ant i -MALIGNIN antibody, whereby the injected RECOGNIN is MALIGNIN or a purified fraction thereof or a structural relative of MALIGNIN which produces or reacts specifically with anti-malignin antibody, whereby said product or a purified fraction thereof preferentially attaches to cancerous cells and can thereby be detected by attached visible or signal- emitting means, said MALIGNIN ςomprising a product, derived from brain tumor cells, which forms a single line precipitate with its specific antibody in quantit¬ ative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometr ic absorption peak wave-length of 280 mμ , a molecular weight of 10,000 and an aminoacid composition approximately as follows: Approximate No. of Residues Asparti c Acid 9 Threonine 5 Ser ine 5 Glutamic Acid 13 Prol ine 4 Glycine 6 Alanine 7 Val ine 6 1/2 Cysteine 1 Meth i on ine 2 I so1eucine 4 Leuci ne 8 Tr yo s i ne 3 Phenylalanine 3 Lysine 6 Hi stidine 2 Arginine 5_ 89 the aminoacids cysteic, hydroxyprol ine, norleucine, anrmonia, i sodesmos ine, lys inonorleucine and garrma- aminobutyric acid being absent in detectable amounts. 8. A process for detecting the presence of cancerous or malignant tumor cells in a cell collection, comprising applying to said cell collection a specific monoclonal anti-cancer RECOGNIN, which preferentially attaches to cancerous cells and can thereby be detected by attached visible or s ignal-emi tting means, said cancer RECOGNIN comprising a product, derived from cancerous tumor tissue or cells, characterised by forming a single line precipitate with its specific antibody in quantit¬ ative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometric absorption peak wave¬ length of 280 mμ and a molecular weight of from 3,000 to 25,000, and further characterized by having an amino- acid residue composition characterized by high proportion of glutamic and aspartic acids and high ratios of glutami and aspartic acids to histidine. 9. The process according to claim 8, wherein said cell collection is j_n vivo. 10. The process according to claim 8, wherein said cell collection is jn Ϊtr_£- 11. The process according to claim 8, wherein the cancer RECOGNIN is MALIGNIN. OMPI 12. The product comprising monoclonal anti- MALIGNIN ant ibody-FAST or a purified fract ion . thereof, whereby said product or a purified fraction thereof preferentially attaches to cancerous cells and can thereby be detected by attached visible or signal- emitting means, said MALIGNIN comprising a product, derived from brain tumor cells, which forms a single line precipitate with its specific antibody in quant¬ itative precipitin tests and Ouchterlony ' gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, and has a spect rophotometr ic absorpotion peak wave-leng-th of 280 mμ, a molecular weight of 10,000 and an aminoacid composition approximately as fol lows : Approximate No. of Residues Aspart ic Acid 9 Threonine 5 Ser ine 5 Glutamic Acid 13 Pro1 ine 4 Glyci ne 6 Alanine 7 Vai i ne 6 1/2 Cysteine 1 Meth i on i ne 2 I so 1 euci ne 4 Leuc i ne 8 Tryos ine 3 Pheny1 alanine 3 Lys ine 6 Hi st idi ne 2 Arg inine 5_ 89 the aminoacids cysteic, -hydroxyprol ine, norleucine, ammonia, i sodesmos ine, 1ys inor leucine and garrma-amino- - 76 - butyric acid being absent in detectable amounts. 13. The product comprising monoclonal anti-MALIGN antibody-SLOW or a purified fraction thereof, whereby said product or a purified fraction thereof preferent¬ ially attaches to cancerous cells and can thereby be detected by attached visible or signal-emitting means, said MALIGNIN comprising a product, derived from brain tumor cells, which forms a single line precipitate with its-specific antibody in quantitative precipitan tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH and insoluble at an alkaline pH, and has a spectrophoto metric absorption peak wave-length of 280 mμ, a mole- cular weight of 10,000 and an aminoacid composition approximately as follows: Approximate No. of Residues Aspartic Acid 9 Threonine 5 Ser ine 5 Glutamic Acid 13 Prol ine 4 Glycine 6 Alanine 7 Val ine 6 1/2 Cysteine 1 Methiόnine 2 Isoleucine 4 Leucine 8 Tryosine 3 Pheny1alanine 3 Lys ine 6 Hi st idine 2 Arginine 5 89 the aminoacids cysteic, hydroxyprol ine, norleucine, anrmonia, i sodesmos ine, 1ys inonor 1eucine and gamma- aminobutyric acid being absent in detectable amounts. 14. The product comprising monoclonal anti- MALIGNIN antibody FAST and SLOW or a purified fraction thereof, whereby said product is cytotoxic to, and kills cancer cells, whereby said product or a purified fractio thereof preferentially attaches to cancerous cells and " can thereby be detected by attached visible or signal- emitting means, said MALIGNIN comprising a product, derived from brain tumor cells, which forms a single line precipitate with its specific antibody in quant¬ itative precipitin tests and Ouchterlony gel diffusion tests, being soluble in.water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometr ic absorption peak wave-length of 280 mμ , a molecular weight of 0 , 000 , and an aminoacid composition approximately as foilows : Approximate No. of Residues. Aspartic Aci d 9 Threonine 5 Ser i ne 5 Glutamic Acid 13 Prol ine 4 Gl ycine 6 Alanine 7 Val i ne 6 1/2 Cysteine 1 Methiόnine 2 I sol eucine 4 Leucine 8 Tryos ine 3 Pheny1al anine 3 Lysine 6 Histidine 2 Arginine 5 89 the aminoacids cysteic, hydroxyprol ine, norleucine, ammonia, i sodesmosine, lysinonorieucine and garrma- aminobutyric acid being absent in detectable amounts. 15. The product according to claim 12, wher.ein said monoclonal anti-MALIGNIN antibody-FAST or a purified fraction thereof, when mixed with the product according to claim 13, monoclonal anti -MALIGNIN anti- body-SLOW or a " purified fraction thereof, whereby said mixture is cytotoxic to and kiljs cancerous cells. 16. The product according to claim 12, wherein said monoclonal ant1 -MALIGNIN ant ibody-FAST is compiexed with any substance which it is desired to have prefer¬ entially attached to cancerous cells. 17. The product according to claim 13, wherein said monoclonal ant i-MALIGNIN ant ibody-SLOW is compiexed with any substance which it is desired to have prefer¬ entially attached to cancerous cells. 18. The product according to claim 14 wherein said monoclonal anti -MALIGNIN antIbody-FAST/SLOW is compiexed with-any substance which it is desired to have prefer¬ entially attached to cancer cells. 19. The product comprising monoclonal anti-cancer RECOGNIN, whereby said ant i-cancer RECOGNIN is produced by the injection into an organism of a cancer RECOGNIN, said cancer RECOGNIN having the ability to induce or to cause cells of said organism to produce specific anti- cancer RECOGNIN, wherein- such anti -cancer RECOGNIN producing cells are subsequently treated to make them sel f-perpetuat ing and identified by their production of monoclonal anti-cancer RECOGNIN, said cancer RECOGNIN comprising a product, derived from cancerous tumor tissue or cells, characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometr i c absorption peak wave¬ length of 280 mμ and a molecular weight of from 3,000 to 25,000 and further characterized by having an amino- acid residue composition characterized by high prop¬ ortions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids,to histidine. 20. The product according to claim 19, wherein said monoclonal anti-cancer RECOGNIN or a purified fraction thereof preferentially attaches to cancerous ceils and can thereby be detected by attached visible or s ignal -emi 11 ing means. 21. The product according to claim 19, wherein said monoclonal anti-cancer RECOGNIN or a purified fraction thereof is cytotoxic to and kills cancerous cells. 22. The product according to claim 19, wherein said monoclonal anti-cancer RECOGNIN or a purified fraction thereof is compiexed with any substance which it is desired to have preferentially attached to cancerous eel 1 s . 23. The product comprising monoclonal anti- MALIGNIN antibody - FAST PRODUCER cell which is charact¬ erized by the ability ' to reproduce itself in perpetuity and to produce monoclonal ' ant i -MALIGNIN antibody - FAST, whereby said PRODUCER cell has had its desoxyr ibon- ucleic acid specifically programmed by MALIGNIN to produce the anti-MALIGNIN antibody, said MALIGNIN comprising a product, derived from brain tumor cells, which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometr ic absorption peak wave-length of 280 mμ, a molecular weight of 10,000 and an aminoacid composition approximately as follows: Approximate No. of Residues Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Prol ine 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Meth ionine 2 Isoleucine 4 Leucine 8 Tryos ine 3 Phenylalanine 3 Lysine 6 Hist idine 2 Arginine 5 89 the aminoacids cysteic, hydroxyprol ine, norleucine, ammonia, i sodesmos ine, 1ys inonor leucine and gamrna- aminobutyric acid being absent in detectable amounts. [RE 24. The product comprising monoclonal anti- MALIGNIN antibody - SLOW PRODUCER cell which is characterized by the ability to reproduce itself in perpetuity and to produce monoclonal anti -MALIGNIN antibody - SLOW, whereby said PRODUCER cell has had its desoxyr ibonucleic acid specifically programmed by MALIGNIN to produce the ant i -MALIGNIN antibody, said MALIGNIN comprising a product, derived from brain tumor cells, which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gell diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectro- photometric absorption peak-wave-length of 280 mμ , a molecular weight of 10,000, and an aminoacid composition approximately as follows: Approximate No. of Res idues Aspart ic Acid 9 Threonine 5 Ser ine 5 Glutamic Acid 13 Prol ine 4 Glycine 6 Alanine 7 Val ine 6 1/2 Cysteine 1 Methionine 2 I so1eucine 4 Leucine 8 Tryos ine 3 Pheny1 alanine 3 Lys i ne 6 Hi s t i d i ne 2 Arg i n i ne 5_ 89 - the aminoacids cysteic, " hydroxyprol ine , nor 1eucine, a mon i a , i sodesmosine, 1ys i nonor 1 euci ne : and gamma- aminobutyric acid being absent in " detectable amounts.. 25. The product comprising monoclonal anti- MALIGNIN antibody - FAST and SLOW PRODUDER cell which is characterized by the ability to reproduce itself in perpetuity and to produce monoclonal ant i -MALIGNIN antibody FAST and SLOW, whereby said PRODCUER cell has had its desoxyr ibonucleic acid specifically programmed by MALIGNIN to produce the anti-MALIGNIN antibody, said MALIGNIN comprising a product, derived from brain tumor cells, which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at ari alkaline pH, and has a spectrophoto- metric absorption peak wave-length of 280 mμ, a mole¬ cular weight of 10,000, and an aminoacid composition approximately as follows: Approximate No. of Residues Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 ProI ine 4 Glycine 6 Alanine 7 Val ine 6 1/2 Cysteine 1 Methion ine 2 - " " I sol eucine 4 Leucine 8 Tryosine 3 Phenyl al an i ne 3 Lys i ne 6 Hi st idine 2 Arginine 5_ 89 the aminoacids cysteic, hydroxypro1 ine, norleucine, ammonia, i sodesmos ine, lys inonor leucine and ganrrna- aminobutyric acid being absent in detectable amounts. 26. The product which comprises the desoxyribon* ucleic acid of Claim 23. 27. The product which comprises the desoxyribon- ucleic acid of Claim 24. 28. The product which comprises the desoxyribon- ucleic acid of Claim 25. 29. The product which comprises the ribonucleic acid of any one of Claims 23 to 25. PI

Inventors: BOGOCH SAMUEL
Applicants: LAREDO JACK JOSEPH
Application year: 1982
Main classification: C12N15
IPC codes: A61K38, A61K39, C07K14, C07K16, C12N5, C12N15, C12P1, C12P21, C12Q1, C12R1, G01N33