THE (R,R)-ENANTIOMER OF TRANS-INDENO(1,2-B)-1,4-OXAZINES, PROCESS FOR ITS PREPARATION AND PHARMACEUTICAL FORMULATION CONTAINING IT

Abstract: The (R₁R)-enantiomers of trans-indeno-[1,2-b]-1,4-­ oxazines demonstrate dopaminergic activity, whereas the (S,S)-enantiomers demonstrate α-adrenergic antagonist ac­ tivity. They are prepared by cyclization of the appropriate chiral 2-amino-1-hydroxyindane.

Description: TITLE OF THE Invention Enantiomers of trans-Indeno 1,2-b]-1,4-oxazines, process for their preparation and pharmaceutical formulation containing them. BACKGROUND OF TIC INVENTION This invention is concerned with the enantiomers of trans-2,3,4,4a,5,9b-hexahydroindeno[1,2-b]-1,4-oxazines of structural formula I which is used in this disclosure to depict the racemic, (S,S)- and (R,R)-enantiomers unless specific entity is meant in which case it is specified to be racemic, or one of the optical isomers. EMI1.1 The (R,R)-enantiomer demonstrates the docaminergic activity of antiparkinson agents, whereas the (8,8) -enantiomer demonstrates the a-adrenergic receptor antagonism of antidepressant agents. The racemic compound is described by Faith et al. in U.S. Patent 3,642,789 where it is alleged to have antidepressant activity by virtue of its reserpine antagonism and amphetamine agonism. Surprisingly it is now found that the (S,S)-enantiomer has only antidepressant activity. Even more surprisingly, it is found that the optical antipode, the (R,R)-enantiomer has a somewhat opposite effect on the CNS being an antiparkinson agent. It is therefore an object of this invention to provide the pure (R,R)-and (8,8)-enantiomers of compound I, processes for preparing the pair of compounds, pharmaceutical formulations, and methods of treating depression with the (S,S) -enantiomer or its pharmaceutical compositions, and methods of treating Parkinsonism with the (R,R)-enantiomer or its pharmaceutical compositions. DETAILED DESCRIPTION OF THE INVENTION The novel compounds of this invention are the (R,R)-, and (S,S)-enantiomer, each substantially free of the other, of the compound of structural formula: EMI3.1 or a pharmaceutically acceptable salt thereof, wherein R is hydrogen; branched or unbranched C1-4 alkyl, especially methyl, ethyl or propyl; C25 alkenyl, especially allyl; or phenyl-C1-4 alkyl, especially beryl; and R1 and R are independently hydrogen, hydroxy, or C14 alkoxy, especially methoxy, or ethoxy. The pharmaceutically acceptable salts of the novel compounds of this invention are acid addition salts formed from a novel compound and an organic or inorganic acid recognized by the art as providing a pharmaceutically acceptable acid addition salt, such as hydrochloride, hydrobromide, dihydrogen phosphate, sulfate, citrate, pamoate, pyruvate, napsylate, isethionate, maleate, fumarate, or the like. These salts are readily prepared by mixing solutions of equimolecular amounts of the free base compound and the desired acid in suitable solvents such as water, alcohols, ether or chloroform, followed by recoverv of the product by collecting the precipitated salt or evaporation of the solvent. The process for preparing the novel compounds is another embodiment of this invention and is represented as follows: EMI4.1 (R,R)- or (S,S) The novel process comprises the reduction of the oxazinone, II, with a metal hydride such as borane, in an inert organic solvent such as an ether, for example, tetrahydrofuran, 1, 2-dimethoxyethane, tetrahydropyran, or the like at a temperature between about OOC and 100 C. Operating procedures normally involve slow addition of the ketone to the reducing agent at room temperature or below, followed by heating to an elevated temperature within the stated range, preferably, the reflux temperature of the solvent, for about 1/2 to about 4 hours usually about 1-2 hours. A third embodiment of this invention is the pharmaceutical formulations comprising one of the novel compounds as active ingredient. They may be in any art recognized form suitable for oral use, such as tablets, troches, lozenges, aqueous or oil suspensions, dispersible powders, or granules, emulsions, hard or soft capsules, syrups, or elixirs. For intravenous and intramuscular and subcutaneous use the pharmaceutical compositions may be in any art recognized form of a sterile injectable preparation such as a sterile aqueous or oleaqinous solution or suspension. The amount of active ingredient incorporated in a unit dosage of the above described pharmaceutical compositions may be from 1 to 400 mg, and preferably from 5 to 250 mg. Other embodiments of this invention are the treatment of depression with the (S,S)-enantiomer and Parkinsonism with the (R,R)-enantiomer of the novel compounds. The route of administration can be oral, rectal, intravenous, intramuscular, or subcutaneous. Doses of 1.0 to 200 mg/kq/day and preferably of 5.0 to 100 mg/kg day of either active ingredient to demonstrate its respective utility are generally adequate, and if preferred it can be administered in divided doses given two to four times daily. It is to be noted that the precise unit dosage form and dosage level depend upon the requirements of the individual being treated, and consequently are left to the discretion of the therapist. EXAMPLE 1 trans-4-methyl 2,3,4,4a,5,9b-hexahydroindeno .[1,2-b1- 1,4-oxazines Step A: Preparation of trans-2-(N-methyl-N acetoacetylamino)-l-indanol (IV) EMI6.1 To a solution of trans-2-methylamino-1indanol (4.9 g, 0.03 m) in 100 ml of ethanol was added 2.8 g (0.033 m) of diketene. After stirring about 1 hour the mixture was concentrated to dryness in vacuo. The residue was dissolved in acetonitrile and the solvent was evaporated in vacuo to 6.3 g (85%) of an oily residue which was used directly in the next step. Step B: Preparation of trans-2-(N-methvl-N-diazo acetoacetylamino) -l-indanol (V) EMI7.1 A mixture of the oilv residue of compound IV from Step A, 100 ml of acetonitrile and 9.0 g (0.031 m) of c-carboxybenzenesulfonyl azide was cooled in ice and treated all at once with 9.0 ml (9.15 g, O m) of 1,8-diazabicyclo [5.4.01 undec-7-ene (DBU) with stirring. A homogeneous solution formed quickly followed by precipitation. The ice-bath was removed and stirring was continued for about 4 hours. Water was added and the mixture was extracted with methylene chloride (3X). The methylene chloride extract was back washed with water (2X), dried over anhydrous sodium sulfate and concentrated to dryness in vacuo leaving a residue of 7.0 g of compound V which was used directly in the next step without purification. Step C: Preparation of 2-(N-diazoacetyl-N-methyl) amino-l-indanol (VI EMI8.1 The residue of structure V from Step B in 100 ml of acetonitrile was treated with 50 ml of 5% (w/v) aqueous sodium hydroxide and the mixture was stirred 5 hours at room temperature. The mixture was partitioned between added water and methylene chloride and the phases were separated. The aqueous phase was extracted again with methylene chloride and the combined methvlene chloride layers were dried over anhydrous sodium sulfate. oncentration to dryness gave 4.55 g of racemic compound VI, m.p. 142-1450C. Step D: Preparation of trans-4-methyl-4,4a,5,9b- tetrahydroindeno[l,2-b]-1,4-oxazin-3-(2H)-one (II) EMI9.1 To a solution of 4.45 g (0.019 m) of 2- (N-diazoacetyl-N-methyl) amino-l-indanol in 200 ml of methylene chloride cooled in an ice-bath there was added dropwise 3.4 g (2.9 ml, 0.023 m) of boron trifluoride etherate. Gas evolution began immediately and the mixture was stirred for 30 minutes. Hydrochloric acid (100 ml of 1 N) was added and stirring was continued for another 30 minutes. The layers were separated and the methylene chloride layer was washed with saturated aqueous sodium bicarbonate solution and water, dried over anhydrous sodium sulfate and concentrated to dryness to give 3.3 g (84%) of racemic compound II, m.p. 154-1620C. Step E: Preparation of trans-4-methyl-2,3,4,4a,5,9b hexahydroindenoti,2-b]-1,4-oxazine (I) EMI10.1 To a solution of 3.3 g (0.016 m) of II in 50 ml of tetrahydrofuran (THF) was added 32 ml (0.032 m) of borane (as 1 M BH3/THF) with ice cooling. vhe mixture was refluxed 1 hour, cooled, treated with 50 ml of 6 N hydrochloric acid and heated on a steam bath until the THF had evaporated. After- cooling the mixture was washed with ether, made basic with 40% (w/v) aqueous sodium hydroxide and extracted with methylene chloride. The extract was dried and concentrated to dryness in vacuo to give 2.4 g (42% overall for 5 steps) of racemic Compound I. Conversion to the hydrochloride gave trans-racemic I.HCl.l/4 H,O: m.p. 252-2550C. Employing the procedures substantially as described in Example 1, Steps A through E, but substituting for the starting material used in Step A thereof, an equimolar amount of the (S,S)-, or (R,R)enantiomer of trans-2-methylamino-1-indanol, there was prepared in comparable yield: trans-(S,S)-I.HCl: m.p. 275-278 C., [&alpha;]D25 + 19.42 (C, 0.556 MeOH); trans-(S,S)-I.maleate: m.p. 146-150 C.; and trans-(R,R) -I.HC1: m.p. 260-2680C., 25 [al -20.38(C, 0.52 MeOH) Employing the procedure substantially as described in Example 1, Steps A through E, but substituting for the trans-2-methylamino-l-indanol used as starting material in Step A an equimolecular amount of the substituted-trans-2-amino-l-indanols described in Table I there are Produced corresponding amounts of the substituted trans-2,3,4,4a,5,9bhexahydroindeno[1,2-b]-1,4-oxazines also described in Table I, in accordance with the following reaction scheme: : EMI11.1 TABLE I Enantiomer R R1 R2 racemic -C2H5 8-OCH3 H (m.p. 243-2450C, (R,R) -C2H5 8-OCH3 H as HC1 salt) (S,S) -C2H5 8-OCH3 H (R,R) -n-C4Hg H H (S,S) -n-C4Hg H H (R,R) -CH2C6H5 H H (S,S) -CH2C6H5 H H (R,R) -C2H5 8-OCH3 7-OCH3 (S,S) -C2H5 8-OCH3 7-OCH3 (R,R) i-C3H7 7-OC2H5 6-0C2H5 (S,S) i-C3H7 7-OC2H5 6-OC2H5 (R,R) -CH2CH=CH2 H H (S,S) -CH2CH=CH2 H H EXAMPLE 2 trans-4-ethyl-6-hydroxy-2,3,4,4a,5,9b-hexahydroindeno (1,2-bi -1,4-oxazine hydrobromide Step A: Preparation of 4-(phenylmethoxy)-l-indanone EMI12.1 To a solution of 4.44 g (0.03 m) of 4-hydroxy-l-indanone in 50 ml of dimethylformamide is added 4.15 g (0.03 m) of potassium carbonate with stirring followed by the dropwise addition of 3.8 g (0.03 m) of benzyl chloride. The reaction mixture is heated at steam bath temperature for 3 hours, then cooled and diluted with water. The aqueous mixture is extracted with ethyl acetate and the extract is washed with water, dried over anhydrous sodium sulfate and concentrated to dryness in vacuo. The residue is triturated with ether and concentrated to dryness to yield: 5.86 q (82%) of a brown solid, m.p. 70-730C, sufficiently pure to be used in the next step. Step B: Preparation of 2-(hydroxyimino)-4-(phenyl methoxy)-l-indanone EMI13.1 To an ice-cooled solution of 1.91 g (0.008 m) of 4-(phenylmethoxy)-l-indanone in 75 ml of anhydrous ether is added dropwise with stirring 1.2 ml (0.0088 m) of isoamyl nitrite while hydrogen chloride is simultaneously bubbled in. After stirring at room temperature for twenty minutes, the reaction mixture is partially concentrated and the solid which separates is filtered to yield 1.83 g (86%) of material sufficiently pure to be used in the next step. Step C: Preparation of 2-(acetylamino}-4-hydroxy-1- indanone EMI14.1 A suspension of 4.94 g (0.018 m) of 2- (hydroxyimino) -4- (phenylmethoxy) -1-indanone in 35 ml each of qlacial acetic acid and acetic anhydride containing 0.7 g of 10t palladium on carbon catalyst is hydrogenated at room temperature until two equivalents of hydrogen are used. The catalyst is filtered off, washed with methanol, and the combined acetic acid-alcohol filtrate is concentrated to dryness in vacuo. The residue is triturated with ethanol and the solid which is filtered is a roughly equal mixture of the 4-hydroxy and 4-(phenylmethoxy) compounds. The mixture is taken up in aqueous sodium hydroxide and the 4-(phenylmethoxy) compound is extracted with ethyl acetate. The basic solution is acidified with aqueous hydrochloric acid and extracted with ethyl acetate. The extract is dried over anhydrous sodium sulfate and concentrated to dryness in vacuo to yield the 4-hydroxy compound. Step D: Preparation of 2-acetylamino-4-(methylthio methoxy) -1-indanone (X) EMI15.1 To 2-acetylamino-4-hydroxy-1-indanone 10.25 g (0.05 m) in 50 ml of dry HMPA under N2 is added 2.6 g (0.055 m) of a 50% oil dispersion of sodium hydride in portions. After stirring at room temperature for 1/2 hr, 5.3 g (0.055 m) of chloromethyl methyl sulfide is added, and the mixture is stirred overnight. Water is added and the mixture is extracted with ethyl acetate which is back-washed with water. After drying (Na2SO4) and concentration, the desired product is obtained. Step E: Preparation of trans-2-ethylamino-4-(methyl thiomethoxy) -l-indanol (XI) EMI15.2 The residue from Step D is dissolved in 50 ml of THF and added to 3.8 g (0.1 m) of 1iAlH4 in 50 ml of THF dropwise. The mixture is refluxed for one hour after completion of the addition. vhe mixture is cooled and sufficient saturated aqueous Na2SO4 solution is added to quench the excess LiAlH4. Methylene chloride and solid anhydrous Na2SO4 is added and the mixture is filtered. Concentration provides the crude product. Step F: Preparation of trans-2-(N-ethyl-N-aceto acetylamino) -4- (methylthiomethoxy) -1-indanol (XII) EMI16.1 Using the product from Step E and following the procedure of Example 1, Step A, with 4.6 g (0.055 m) of diketone in 200 ml ethanol, the desired product is obtained. Step G: Preparation of trans-2-(N-ethyl-N-diazoaceto acetylamino) -4- (methylthiomethoxy) -1-indanol (XIII) EMI16.2 Using the product in Example 1, Step B, with 11.3 g (0.05 m) of p-carboxybenzenesulfonylazide in 150 ml of acetonitrile, the desired product is obtained. Step H: Preparation of trans-2- (N-ethyl-N-diazo- acetYlamino)-4-(methylthiomethoxy}-l-indan (XIV) EMI17.1 Using the product from Step G and following the procedure in Example 1, Step C, with 100 ml of acetonitrile and 50 ml of 5% (w/v) aqueous sodium hydroxide and stirring overnight, the desired product is obtained. Step I: Preparation of trans-4-ethyl-6-(methylthio methoxy)-4,4a,5,9b-tetrahydroindeno[1,2-b] 1,4-oxazin-3-(2H) -one (XV) EMI17.2 Using the product from Step H and following the procedure in Example 1, Step D, with 6.4 g (5.5 ml, 0.045 m) of boron trifluoride etherate in 200 ml of methylene chloride, the desired product is obtained. Step J: Preparation of trans-4-ethyl-6-hydroxy 4,4a,5,9b-tetrahydroindenol1,2-b]-1,4-oxazin- 3-(2H)-one (XVI) EMI18.1 To the product from Step I in 200 ml of a 4:1 (v/v) acetonitrile-water mixture is added 12.2 g (0.045 m) of mercuric chloride. The resulting suspension is refluxed for 10 hr, diluted with methylene chloride, and filtered through celite. The organic phase is washed with water, dried over Na2SO4, and concentrated. The residue is taken up in methanol, aqueous 48% HBr is added, and the solvents are removed in vacuo. Methanol is added and the mixture reconcentrated several times to remove any water. Ethanol is added and ether is used to precipitate the desired HBr salt of the product. Step K: Preparation of trans-4-ethyl-6-hydroxy 2,3,4,4a,5,9b-hexahydroindeno[l,2-bi-l,4- oxazine (I) EMI19.1 Using the product from Step J and following the procedure in Example 1, Step E, with 45 ml (0.045 m) of borane (as a 1M BH3/THF solution) in 100 ml of THF, the desired product is obtained. If compound XI, the product of Step E, is resolved into its (R,R)- and (S,S)-enantiomers and each is independently subjected to the chemical reactions described in Steps F through K, there are produced respectively, the (R,R)- and (S,S)enantiomers of trans-4-ethyl-6-hydroxy-2,3,4,4a,5,9b hexahydroindeno[l,2-bl-1,4-oxazine. Employing the procedures substantially as described in Example 2, Steps A through K but substituting for the starting material used therein equimolecular amounts of the hydroxy-l-indanones described in Table II, there are produced the racemic, (R,R)- and (S,S)-enantiomers of the substituted trans-2,3,4,4a,5,9b-hexahydroindeno- [1,2-b]-1,4-oxazines, also described in Table II. TABLE II EMI20.1 EMI20.2 <tb> R1 <SEP> R2 <SEP> R <SEP> R1 <SEP> R2 <tb> 4-OH <SEP> -C2H5 <SEP> 6-OH <tb> 4-OH <SEP> 5-OH <SEP> -C2H5 <SEP> 6-OH <SEP> 7-OH <tb> 6-OH <SEP> -C2H5 <SEP> 8-OH <tb> 5-OH <SEP> 6-OH <SEP> -C2H5 <SEP> 7-OH <SEP> 8-OH <tb> 4-OH <SEP> n-C3H7 <SEP> 6-OH <tb> 4-OH <SEP> -n-C4H9 <SEP> 6-OH <tb> 7-OH <SEP> -C2H5 <SEP> 9-OR <SEP> <tb> EXAMPLE 3 Pharmaceutical Composition A typical tablet containing 100 mg of active ingredient per tablet is prepared by mixing together with the active ingredient, calcium phosphate, lactose and starch in the amounts shown in the table below. After these ingredients are thoroughly mixed, the appropriate amount of magnesium stearate is added and the dry mixture blended for an additional three minutes. This mixture is then compressed into tablets. Tablet Formula Ingredient Mg. per Tablet Trans-(R,R)-4-methyl-2,3,4,4a, 5,9b-hexahydroindeno[1,2-b]-1,4-oxazine 100 mg Calcium phosphate 52 mg Lactose 60 mg Starch 10 mg Magnesium Stearate 1 mg Similarly prepared are tablets comprising as active ingredient any of the other novel compounds described herein.

Claims: WHAT IS CLAIMED IS: 1. A process for the preparation of the (R,R)- and (S,S)-enantiomers of a comPound of structural formula I, each substantially free of the other: EMI22.1 whereine R is C1-4 alykl, C2-5 alkenyl or phenyl C1 4alkyl; and R and R2 are independently hydrogen, hydroxy, or C1 4alkoxy; which comprises reduction of the (R,R)- and (S,S)-enantiomers, respectively, of a compound of structural formula: EMI22.2 2. The process of Claim 1, wherein R is methyl or ethyl, and R1 and R2 are independently hydrogen, methoxy or hydroxy. 3. The (R,R3-enantiomer of a compound of structural formula I, substantially free of the (S,S)enantiomer EMI23.1 (R,R)-enantiomer or a pharmaceutically acceptable salt thereof, wherein R is C14alkyl, C25 alkenyl or phenyl-Cl 4alkyl; and R1 and R are independently hydrogen, hydroxy or C1-4 alkoxy. 4. The compound of Claim 3 wherein R is methyl, ethyl or propyl, and R1 and R2 are independently hyrogen, methoxy or hydroxy. 5. The (S,S)-enantiomer of a compound of structural formula I, substantially free of the (R,R)enantiomer: EMI23.2 (S,S)-enantiomer or a pharmaceutically acceptable salt thereof, wherein R is C1-4 alykl, C2-5 alkenyl or phenyl-C1-4 alkyl; and R1 and R2 are independently hydrogen, C1 4alkoxy or hydroxy. 6. The compound of Claim 5 wherein R is methyl, ethyl or propyl, and R1 and R2 are independently hydrogen, methoxy or hydroxy. 7. A pharmaceutical formulation for use as an antidepressant comprisinq a pharmaceutical carrier and an effective antidepressant amount of the (S,S)-enantiomer of a compound of structural formula: EMI24.1 (S, S) -enantiomer or a pharmaceutically acceptable salt thereof, wherein R is C1-4 alkyl, C2-5 alkenyl or phenyl-C14alkyl; and R1 and R2 are independently hydrogen, hydroxy or C1-4 alkoxy. 8. The formulation of Claim 7 wherein R is methyl, ethyl or propyl, and R1 and R2 are independently hydrogen, methoxy or hydroxy. 9. A pharmaceutical formulation for use as an antiparkinson agent comprising a pharmaceutical carrier and an effective antiparkinson amount of the (R,R)-enantiomer substantially free of the -(S,S)-enantiomer of a compound of structural formula: EMI25.1 (R,R)-enantiomer or a pharmaceutically acceptable salt thereof, wherein R is cl¯4alkyl C2-5 alkenyl or phenyl-C1-4 alkyl; and R1 and R2 are independently hydrogen, hydroxy or C1-4 alkoxy. 10. The formulation of Claim 9 wherein R is methyl, ethyl or propyl, and R1 and R2 are independently hydrogen, methoxy, or hydroxy.

Inventors: JONES, JAMES H., MCCLURE, DAVID E.
Applicants: MERCK & CO. INC.
Application year: 1982
Main classification: C07D265
IPC codes: A61K31, A61P25, C07D265, C07C45