COPOLYESTERS AND PROCESS FOR THEIR PRODUCTION

Abstract: High molecular weight copolymers containing 3-­ hydroxybutyrate residues, i.e. units of the formula -O.CH(CH₃).CH₂.CO- and up to 50 mole % of residues of other hydroxy acids, viz units of the formula -O.CR¹R².(CR³R⁴) n .CO- where n is 0 or an integer and, if n = 1 and R², R³, and R⁴ = H, R¹ is not methyl. The copolymers are made microbiologically: for part of the cultivation the micro-organism is under conditions such that polymer is accumulated, e.g. by limitation of a nutrient, e.g. nitrogen source, required for growth but not polyester accumulation. For at least part of this period of polymer accumulation the substrate is an acid or a derivative thereof that gives the comonomer units. Propionic acid, which gives polymers where n = 1, R²=R³=R⁴=H and R¹ = C₂H₅, is the preferred acid.

Description: 3-hydroxybutyrate Polymers This invention relates to 3-hydroxybutyrate polymers hereinafter ref erred to as PHB. PIB is accumulated by various micro-organisms, princip- ally bacteria, as an energy reserve material as granules within the microbial cells. PHB extracted from such cells is a thermoplastic polyester of the repeat structure - O.CH(CH3)#CH2#CO- that rapidly crystallises to a relatively high level e.g. of the order of 70% or more. This crystallisation behaviour is often disadvantageous when the polymer is to be used as, for example, a moulding material. We have found that the crystallisation of PHB can be modified by incorporation of units of a dissimilar monomer into the polymer chain. Thtis a minor proportion of comonomer units may be introduced into the polymer chain by cultivation of the micro-organism under certain conditions in the presence of certain organic acids. One example of such an acid is propionic acid. although we do not wish to be bound by the following theory, it is thought that the metabolic pathway leading to such copolymers is as follows, in which CoASH is unesterified Coenzyme A, (So CH3.CO.S.Cot is the acetyl thioester of Coenzyme A and is more commonly termed acetyl CoA), and NADP is nicotinamide adenine dinucleotide phosphate in the oxidized state. NADPH3 is reduced NADP. It is believed that, in the biosynthesis of PHB by a micro-organism the first step is the synthesis of acetyl CoA. This can be formed, for example, from Coenzyme A and acetate, or by the decarboxylation of pyruvate, which is a product of the glycolysis of carbohydrates, or which can be formed by decarboxy- lation of oxaloacetate, the latter being a member of the tricarboxylic acid, TCA, cycle, otherwise known as the Krebs cycle. Thus with acetate as the source of acetyl CoA, the PHB is produced by a metabolic pathway involv ng the reactions: EMI2.1 <tb> 1. <SEP> GH3.CO.0 <SEP> + <SEP> CoA.SR <SEP> iokinase <SEP> > <SEP> CR <SEP> CO.S.CoA <SEP> + <SEP> OR <tb> 2. <SEP> 2C:CO,S,CoB <SEP> Sketothiolase <SEP> C'Hj. <SEP> CO. <SEP> CO.CR2CO0S.CoA <SEP> + <SEP> CoA.SH <tb> 3. <SEP> CH3oCOsCNoCOoSoCOX <SEP> + <SEP> NA <SEP> G <SEP> teductase <tb> CH3#CHOH#CH2#COäS#CoA + NADP 3-hydroxybutyryl CoA EMI2.2 <tb> 4. <SEP> CH30CHOEOCH2 CO*SXCoA <SEP> ¯olymeraBe <SEP> -O.CE(CH3).CH2.CO- <SEP> + <SEP> CoASR <tb> repeat unit in polymer Thus reaction 4 adds a -O.CH(CH3) #CH2#CO- unit to a growing polymer chain. Because of lack of specificity of the enzymes involved, with propionic acid, the corresponding pathway is thought to be: EMI2.3 i.e. repeat units having a pendant ethyl group are introduced into the polymer chain: in contrast P33 has pendant methyl groups. Certain polymers containing 3-hydroxybutyrate units, i.e. O.CH(CH3)#CH2#CO- units, together with other units have been described in the literature. Thus polymers exhibiting an infra-red band said to be indicative of ethylenic unsaturation are described by Davis in "Applied Microbiology" 12 (1964) pages 301 - 304. These polymers which are said by Davis to be copolymers containing 3-hydroxybutyrate units and 3-hydroxy-2-butenoate units, i.e. units of the formula - 0.C(CR3) = CR.C0 - were prepared by cultivating Nocardia on n-butane. Also Wallen et al describe in "Environmental Science and Technology" 6 (1972) pages 161 - 164 and 8 (1974) pages 576 - 57 a polymer melting at 97 - 100 C (after repeated washing) isolated from activated sludges and containing 3-hydroxybutyrate units --d 5-hydroxyvalerate units,i. e. -O.CH(C2H5)#CH2#CO- units in the ratio of 1:5. The polymer thus contains only about 17% of 3-hydroxybutyrate units. Marchessault et al report in "TUPAC Macro Florence 1980 International Symposium on Macromolecules Preprints" 2 (1980) pages 272 - 275 a study of this polymer and confirmed that it contained mainly 3-hydroxyvalerate units. United States Patent Specification 3275610 describes the microbiological production of polyesters by cultivating certain micro-organisms, especially Nocardia salmonicolor, on carboxylic acids containing 4 carbon atoms. In samples 2 and 3, where the acids were 3-buteroic and 2-hydroxybutyric acids respectively, the polymers appear, from the quoted melting points of the order of 178 - 184 C, to be poly(3-hydroxybutyrate). In Example 1 however, wherein 2-methyl acrylic acid, i.e. methacrylic acid, was employed the polymer produced is unidentified but is described as having a melting point of 215 - 2200C and as being soluble in methyl ethyl ketone. In contrast thereto, copolymers in accordance with the present invention, containing predominatly 3-hydroxybutyrate residues, have melting points below 180 C and are insoluble in cold methyl ethyl ketone. When PHB-accumulating micro-organisms are aerobically cultured on a suitable substrate, i. e. a source of energy and carbon, they reproduce until one or more of the essential requireents for reproduction is exhausted. This reproduction of the micro-organism is hereinafter referred to as growth. Upon exhaustion of an essential growth requirement, further growth occurs only to a very limited extent, if at all, but, providing the substrate is not exhausted, PEB may be accumulated by the micro-organism. With some micro-organisms, even in the absence of a PHB- inducing constraint such as a limitation on one or more of the essential growth requirements, FS may also be accumulated while growth of the micro-organism is taking place: however, except in the case of ricro-organisms that produce PHB constitutively, the amount of P± so accumulated is generally small and typically is less than about 10% by weight of the cells produced. Thus when grown in batch culture, the micro-organisms that do not produce P constitutuvely, will grow, with little or no P33 accumulation1 until one or more of the essential requirements for growth becomes exhausted, and then the micro-organism synthesises PB. In order to produce copolymers it is therefore necessary to use the acid (or a derivative thereof) that is to give rise to the copolymer units other than 3-hydroxybutyrate units as at least part of the substrate present during the period when polymer is accumulated. The acid, or derivative thereof, that gives rise to the copolymer units other than 3-hydroxybutyrate units is herein teed the comonomer component of the substrate. When the cultivation conditions are such that the polyester, e.g. PRB, is not being accumulated to any significant extent, the comonomer component of the substrate will often be metabolised by the micro-organism by other pathways leading to e.g. acetyl CoA or to a member of the TCA cycle, and so copolymers will not be produced. Thus, as an example, propionic acid can be metabolised by micro-organisms, in the absence of any growth limitation, via propionyl CoA, with the incorporation of carbon dioxide to methyl malonyl CoA, and thence to succinate, a member of the TCA cycle. Metabolism of the comonomer component of the substrate by such other pathways may also occur when using micro-orgarlisms that produce PHB constitutively. Rence we prefer, even when using constitutive PSaccumulating micro-organisms to cause the polymer to be accumulated by cultivation of the micro-organism under conditions wherein the amount of one or more of the essential requirements for growth, but not PHB accumulation, is limited. ivan when cultivating the micro-organism under conditions where there is a restriction of an essential requirement for growth, so that polymer is accumulated by the micro-organism, some of the comonomer component of the substrate may be metabolised by pathways leading to acetyl CoA or a member of the TCA cycle. This enables the micro-organism to synthesise 3-hydroxybutyrate units for incorporation into the copolymer as well as the dissimilar copolymer units, even if the comonomer component is the sole substrate during the polymer accumulation stage. Also, as indicated by the metabolic pathway suggested above for the production of 3 hydroXrvalerate units from propionate, one step, reaction 2a, involves the reaction of propionyl Cos with acetyl CoA. Hence if propionate is the sole substrate, some of the propionate is metabolised to acetyl CoA in order that 3-hydroxyvalerate units can be produced. In addition to the metabolic pathways shown above, leading to 3-hydroxyvalerate units in the copolymer, other reactions can occur with various other materials as the comonomer component of the substrate. For example it is possible that other hydroxy substituted carboxylic acids, if not metabolised by other routes leading to e.g. acetyl or propionyl CoA, could in some cases be incorporated directly by the polymerase into the polymer, e.g. EMI5.1 where n is O or an integer and Rl, Rê, Rê, R4, which may be the same or different, are selected from hydrocarbon radicals, such as alkyl, arallvl, aryl, or alkaryl radicals; halo- and hydroxysubstituted hydrocarbon radicals; hydroxy radicals; halogen atoms; and hydrogen atoms. Of course where n = 1 and Rê = R3 = R4 hydrogen, if RÚ is methyl the hydroxycarboxylic acid is 3-hydroxybutyric acid which of course will be directly assimil ated to give 3-hydroxybutyrate units. Preferably the groups R, R2, R3 and R4 each contain less than 4 carbon atoms. Generally at least one of the groups R, R2, R3 and R4 is hydrogen. n is preferably 0, 1 or 20 Such hydroxy-carboxylic acids may be added as such (as part, or all, of the comonomer component of the substrate) or may by synthesised by the micro-organism from other comonomer component materials. In particular we have found that acids such as acrylic, 3-chloropropionic, and 3-hydroxypropionic acids may give rise to polymers containing 3-hydroxybutyrate units and other units. In some cases all these other units are 3-hydroxyvalerate units while in other cases, the other units which are hereinafter termed units A and which may occur in conjunction with 3-hydroxyvalerate units, are characterised by proton and 3C nuclear magnetic resonance spectra exhibiting, inter alia, (i) a proton NNR triplet at 4.3 ppm, and (ii) 13C NMR peaks at 59.89 and 33.83 ppm (relative to a tetramethyl silane standard). From these NMR data it is believed that these units are 3-hydroxy- propionate units (i. e. n = 1; RÚ = Rê = R = R4 = The copolymers may also contain 4-hydroxyvalerate units i. e. n = 2; RÚ = CH3; Rê = R = R4 = H. 3-Hydroxypropionate and/or 4-hydroxyvalerate units could result from the inte=ediates CH3OH.CH3.CO.S.CoA and CH3.CHOH.CH2.CH2.CO.SCoA. The former could be formed from 3 hydrorypropionate and CoA.SE. In addition to being supplied as such, 3-bydroxypropionate could be formed by the hydration of acrylate: EMI7.1 or by the hydrolysis of 3-chloropropionate EMI7.2 4-hydroxyaaleryl CoA could be derived from the condensation of acetyl CoA and acrylyl CoA followed by reduction, i.e. EMI7.3 Similarly 4-hydroxyvaleryl CoA could be produced from 3-hydroxy-, or 3-chloro-propionate by reactions involving condensation with acetyl CoA, reduction, and dehydration (with an intermediate hydrolysis step in the case of 3-chloropropionate). By the process of the present invention it is therefore possible to obtain copolymers containing 3-hydroxybutyrate units. I -O.CH(CH3).CH2.CO.together with units of the formula II -O.CRÚRê.(CR R4)n.COwhere n is O or an integer and R1, R2, R3, and R4 are each selected from hydrocarbon radicals, such as alkyl, aralkyl, aryl, or alkaryl radicals; halo- and hydroxy- substituted hydrocarbon radicals; hydroxy radicals; halogen atoms; and hydrogen atoms, provided that, where n is 1, if Rê, R and R4 are each hydrogen atoms, R1 is not methyl. Preferably the groups R1, R2, R3 and R4 each contain less than 4 carbon atoms" Generally at least one of the groups R1, R2, R3 and R4 is hydrogen. Preferably n is 0, 1 or 2. The copolymers may contain more than one type of unit II. Preferably in at least some of the units II n = 1, R2 B3 = R4 = R and R1 = ethyl. To be of any practical use as plastics materials, the polymers should have a weight average molecular weight, (Mw), above 10,000 e.g. as measured by gel permeation chromatography. The proportion of repeat units II in the copolymer is preferably between 0.1 and 50, particularly 1 to 40, mole percent of the total repeat units in the copolymer. Ln some cases the polymer produced by the micro-organism may be a blend of homopolymer of repeat units I with a copolymer containing repeat units I and II. In this case the overall proportion of repeat units II in the polymer is preferably between 0.1 and 50 mole per cent of the total repeat units. Most preferably the proportion of repeat units II in the polymer is between 3 and 30 mole 5'. In order to obtain a signifieant proportion of comonomer units II, the amount of combined carbon in the comonomer component of the substrate should be at least 2%,preferably at least 10% by weight of the total combined carbon in the substrate present during the period when the cultivation conditions are such that polymer is being accumulated by the micro-organism. During this period, we prefer that the comonomer component is the only carboxylic acid (or derivative thereof) present in the substrate, although in some cases acetate may also be present. According to the present invention we therefore provide a process for the production of a thermoplastic polyester wherein a micro-organism that is capable of accumulating a polyester is cultivated in an aqueous medium on a water soluble assimilable carbon containing substrate with at least part of the cultivation being conducted under conditions causing polyester to be accumulated by the micro-organism characterised in that, at least during part of the period when polyester is being accumulated, the substrate comprises an organic acid, or derivative thereof, that is metabolisable by said micro-organism under said polyester accumulating conditions, to a polyester other than one composed solely of -O.CE(CE3).CE2.COu repeat unit., the amount of combined carbon ia said acid, or derivative thereof, constituting at least 2, 6 by weight of the total combined carbon in the substrate present throughout said period. The concentration of the comonomer component in the aqueous median should be above 0.05 g/l and will preferably be at a level between 0.1 and 5 g/l. It will therefore be appreciated that the water solubility of the comonomer component should be above 0.05 g/l and sufficient to provide the desired level of the comonomer component concentration at the cultivation t emp erature. The comonomer component may be the acid itself or a salt, ester (including a lactone in the case of a hydroxy substituted acid), annydride, amide or halide. As mentioned hereinbefore, it is preferred that at least part of the cultivation is conducted under conditions of limitation of an essential requirement for growth but not polyester accumulation. The most convenient growth requirement limitation is limitation of nitrogen. For this reason, where nitrogen limitation is employed, the substrate is preferably nitrogen free and so amides are less preferred substrates. Acids that can be used to produce copolymers should be those that do not give rise only to repeat units I, when the cultivation is in the polymer accumulating stage. Unsuitable acids therefore include acetic and Shydroxybutyric acids, members of the TCA cycle, and acids giving only acetyl CoA and/or a member of the TCA cycle when the cultivation is in the polymer accumulating stage. Thus unsuitable acids also include phosphoglyceric, pyruvic, citric, isocitric, q-ketoglutaric, succinic, fumaric, maleic, malic, oxalacetic, oxalosuccinic, aconitic, and methyl malonic, acids. Amino acids are likewise unsuitable, Other acids that have been found not to give copolymers include formic, butyric, phenyl acetic, benzoic, chloroacetic, 2-chioropropionic, 3-hydroxybutyric, 4-hydroxybutyric, 2-chlorobutyric, 2-methyl acrylic, 2, 3-dimethylacrylic, 3,3-dimethylacrylic, lactic, glyoxylic and glycolic acids. Acids that have been found to give copolymers include prorionic, 3-hydrorypropionic, 3-chloropropionic, 3-ethoxypropionic, 2-hydroxybutyric, isobutyric, and acrylic acids. Other acids that could be used include higher saturated carboxylic acids containing an odd number of carbon atoms, e.g. valeric and heptanoic acids; pivalic acid; and substituted propenoic acids, eegO 2- and 3chloropropenoic acids. is mentioned hereinbefore, in some cases the microorganism may perform further reactions on the acid: thus isobutyric acid would be expected to give repeat units II in which n is 1, R2 = - = R4 = H, and R1 is isopropyl. In fact repeat units of the type II wherein n is 1, R2 - R3 = R4 = H, and R1 is ethyl are found indicating that the micro-organism here substitutes hydrogen for a methyl group during the metabolic pathway to the copolymer. Preferred acids that can give rise to copolymers are propionic, isobutyric, and acrylic acids. Suitable derivatives of such acids include the alkali metal salts thereof and the lower alkyl esters (in which the alkyl group contains 1 to 4 carbon atoms). Particularly suitable esters include methyl, ethyl, isopropyl, propyl, butyl and isobutyl prooionates; methyl and ethyl isobutyrates; and methyl end ethyl acrylates. Mixtures of copolymer forming acids (or derivatives thereof) may be used as the comonomer component of the substrate For example interesting results have been obtained using a mixture of propionic and acrylic acids. As indicated above, it is preferred even when using a micro-organism that produces PHB constitutively, to conduct the period of cultivation of the micro-organism where polyester is being accumulated under conditions of limitation of a nutrient required for growth but not polyester accumulation. In addition to the substrate and oxygen (which is generally supplied by injecting air into the aqueous medium in the Sermenter), various nutrient salts are required to enable the micro-organism to grow. Thus source. of the following elements in assimilable form, normally as water soluble salts, are generally required: nitrogen, phosphorus, sulphur, potassium, sodium, magnesium, calcium, and iron, together with traces of elements such as manganese, zinc and copper. While it may be possible to induce polyester accumulation by restricting the supply of oxygen to the fermenter, it is preferred to restrict the amount of one or more of the nutrient salts. The most practical elements to limit are nitrogen, phosphorus, or, less preferably, magnesium, sulphur or potassium. Of these it is most preferred to restrict the amount of nitrogen (which is conveniently supplied as an ammonium salt). The amount of assimilable nitrogen required is about 8 - l50A by weight of the desired weight of cells less accumulated polyester. The fermentation is preferably conducted so that the dry weight of the polyester-containing cells is at least 5 g per litre of aqueous medium. Hence if, for example, it is desired to produce 10 g per litre of PH3containg cells having a PH3 content of 4096 by weight, the amount of the essential nutrient fed to the fermenter that is used to limit the amount of cell growth must be that required to support the growth of 6 g per litre of cells containing no PHB: : thus, if nitrogen is employed as the growth limiting nutrient, since the nitrogen content of PRB free bacterial cells is about 8 - 15% by weight, the amount of assimilable nitrogen required would be between about 0.5 and 0.9 ,g per litre, e.g. about 0.6 to 1.2 g of ammonium ions per litre. The fermentation may be conducted under the conditions e.g. pH, temperature, and degree of aeration (unless oxygen is utilised as the limiting nutrient) conventionally used for the micro-organism. Likewise the amounts of nutrient salts (other than the growth limiting nutrient whose amount may be determined following the considerations outlined hereinbefore) employed may be those normally used for growth of the micro-organism. The micro-organism is preferably grown to a certain desired weight by cultivation in the presence of sufficient of the nutrient required for growth that is to be restricted in the polymer accumulation stage on a readily metabolisable substrate, such as a carbohydrate, and then cultivated under conditions of growth requirement restriction to cause the polymer accumulation. In some cases the substrate for at least part, and in some cases all, of the growth stage may be the acid (or derivative thereof) that gives rise to the copolymer repeat units II in the polymer accumulation stage. The fermentation may be performed as a batch fermentation in which case polymer accumulation will occur as the amount of the nutrient that is required for growth but not polymer accumulation becomes depleted, i.e. exhausted. Alternatively the fermentation may be conducted as a continuous process wherein aqueous medium containing the bacterial cells is removed, continuously or inter mittently, from the fermentation vessel at a rate corresponding to the rate of addition of fresh aqueous medium and substrate thereto. It is preferred that the amount of the nutrient that is restricted that is fed to the fermentation vessel is such that the aqueous medium removed from the vessel contains little or none of that nutrient, and the aqueous medium removed from the vessel is then fed to a second fermentation vessel, operated either in batch or, preferably, continuous fashion wherein polymer accumulation is caused to take place by continuing the aerobic cultivation with the addition of a fresh quantity of substrate comprising the comonomer component. While additional quantities of substrate and nutrient salts may be added in this further fermentation step, since further growth is generally not desired, little or no further quantity of the nutrient utilised to limit growth should be added. It will however be appreciated that the aqueous medium fed to the further fermenter or fermenters from the first fermenter may contain some residual quantity of the limiting nutrient and/or the addition of a further small quantity thereof may be desirable for efficient operation. Alternatively the fermentation may be conducted as a single stage continuous process. In order to achieve polyester accumulation by means of nutrient limitation the residence time of the medium in the fermenter is made sufficiently long to allow the micro-organism to grow and exhaust the limiting nutrient supplied to the fermenter and to allow the micro-organism then to accumulate the polyester. In either a batch process, or continuous processes as described above, the acid (or derivative thereof) used to provide the copolymer repeat units II is used as part, or all, of the substrate during the polymer accumulation stage occurring upon exhaustion of the nutrient required for growth The comonomer component may be used in admixture with a substrate, e.g. a carbohydrate, that will give repeat units I, or may be the sole substrate: in the latter case sufficient of the comonomer component will normally be metabolised by other pathways to acetyl CoA to provide the repeat units I and any acetyl CoA required to produce the repeat units II, e.g. if a pathway involving reaction 22, is employed. However, when the comonomer component is the sole substrate, the yield of polymer is often low. The comonomer component may be present for only part of the polymer accumulation stage: for the rest of the polymer accumulation stage, which may occur before mnd/or after the part of the polymer accumulation stage wherein the comonomer component is present, a substrate giving only repeat units I may be the sole substrate. In some cases it may be possible to prevent the "normal " metabolism of the comonomer component, i.e. to acetyl CoA, by blocking enzymes required for that pathway and/or by using microorganisms that lack the ability to synthesise the necessary enzymes. However in order to obtain substantial yields of polymer a period of cultivation under conditions of limitation, and preferably depletion, of a nutrient required for growth is generally desirable. I The fermentation is preferably conducted so that the amount of accumulated polyester comprises about 50 to 80% by weight of the bacterial cells. Mcro-organisms that may be used include any poly (3-hydroxybutyrate accumulating micro-organisms that are capable of assimilating the acid (or derivative thereof) from which it is desired to produce the copolymers. The bacteria Alcaligenes eutrorkus (previously known as Eydrogenomonas eutrorha) species, e.g. strain H 15 widely employed in academic studies of this species, see e.g. J General Microbiology (1979) 115 pages 185 192, and which is available as ADCC strain 17699, and mutants of strain H 16 such as mutants 11/7B, S301/C5, S501/C29 and S501/C41, which have been deposited, on i8 August 1980 with the National Collection of industrial Bacteria, Torry Research Station, Aberdeen, Scotland, under NCIB Nos. 11500, 11599, 11597 and 11598 respectively, are narticularly suitable. The ATCC number refers to the number designated by the American Type Culture Collection, 12301 Park Lawn Drive, Rockvilles Maryland 20852, U.S.A. As mentioned hereinbefore a carbohydrate is preferably used as the substrate during the growth stage. While Alcaligenes eutronhus strain R 16 (ATCC 17699) will not utilise glucose, certain mutants thereof, e.g. the aforesaid mutants 11/7B, S301/C5, S501/C29 and S501/C41 can utilise glucose. Carbohydrates, particularly glucose, are the preferred substrates for the growth stage in view of the cost and the fact that the micro-organisms can grow efficiently thereon. The polyester is produced as granules inside the microorganism cells. While the cells containing the polyester may themselves be used as a moulding material, for example as described in USP 3,107,172, it is generally desirable to separate the polyester from the bacterial cells. This may be accomplished by subjecting the cells to a cell breakage step followed by extraction of the polyester with a suitable solvent. Examples of suitable extraction processes are described in our European Patent Application 15123. As mentioned hereinbefore the copolymers should have a weight average molecular weight (Mw) above 10,000 as measured by gel permeation chromatography, if they are to be of any practical use Preferably Mw is above 50,000, more preferably above 100,000 and in particular above 200,000. The copolymers invariably have the D-configuration and exhibit melting points below that of the hydroxybutyrate homopolymer. The copolymers are of particular utility in the preparation of melt-fabricated articles, where their reduced crystallinity compared to Shydroxsbutrrate homopolymer is often advantageous. Of particular interest is the use of small amounts of the copolymers as high molecular weight processing aids for vinyl chloride polymers. For this application the amount of copolymer is preferably 0.5 to lC96 by weight of the vinyl chloride polymer. For the best results in this application, we have found that the Co- polymer should be random: to obtain random copolymers, the comonomer component used to produce the comonomer units II is preferably the sole substrate present, at least throughout the period of cultivation of the micro-organism under the conditions of polymer accumulation. Copolymers are also of particular utility in the production of film by melt extrusion, preferably followed by rolling, e.g. passage through one or more pairs of rolls, to reduce the film thickness and induce some orientation, at a temperature between the glass transition temperature (Tg) and the melting point of the polymer. The invention is illustrated by the following examples. FXAMPLE 1 In the normal metabolism of propionate, the latter is converted, as described hereinbefore, to succinate which can give rise to acetyl CoA by oxidation in the TCA cycle to oxaloacetic acid followed by decarboxylation. In the decarboxylation of oxaloacetic acid both terminal acid groups are removed as carbon dioxide. Hence if propionate having the carbon atom of the carboxyl group radio labelled, i.e. 1 - 14C propionate, is supplied to the cells conversion to acetyl CoA will result in loss of the radioactivity as 14CO2. Any incorporation of 14C into the polymer must result from conversion of propionyl CoA into 3-hydroxyvaleryl CoA and subsequent polymerisation. Alcaligenes eutrophus mutant NCIB 11599 was grown by aerobic cultivation in a batch fermenter employing an aqueous medium A which contained sufficient assimilable nitrogen to support a biomass free of accumulated polyester of 3.5 g 1 1 and glucose as the substrate. Medium A had the composition, per litre of deionised water: (NH4)2SO4 2 g MgSO4#7H2O 0.8 g K2SO4 0.45 g R3P04 (1.1 M) 12 ml FeS04.7R20 15 mg Trace element solution 24 ml The trace element solution had the following composition, per litre of de-ionised water Cus04.sR20 0.02 g ZnS04.6H20 0.1 g MnS04.4H20 0.1 g CaCl2#2H2O 2.6 g When the biomass concentration reached 4.5 g 1 , i.e. after the system became starved of assimilable nitrogen, 1 g 1-1 of sodium propionate containing 1-1 C- propionate was added to the fermenter in addition to glucose and fermentation continued for 5 minutes. The cells were then harvested by filtration and the polymer extracted with chloroform. The labelled carbon was found almost exclusively in the chloroform solution indicating that the labelled terminal carbon atom had not been lost as carbon dioxide. Hence at least some propionate had been incorporated into the polymer other than as acetyl CoA. EXAMPLE 2. (comparative) Alcaligenes eutrophus mutant NCIB 11599 was grown by aerobic cultivation at per 6.8 and 340C in a 5 litre batch fermenter containing 4000 ml of an aqueous medium B having the composition, per litre of deionised water: (NH4)2S04 4 g MgSO4.7H20 0.8 g K2 SO4 0.45 g H PO (1.1 M) 12 ml 34 FeS04.7H20 15 mg Trace element solution (as used in Example 1) 24 ml Glucose was fed to the fermenter at a rate of 8 g hr The amount of assimilable nitrogen in medium B was sufficient to support 26 g of PRB-free cells. After 40 hours the cells were harvested by centrifugation; they were then freeze dried and the polymer extracted with chlorofonr. EXAMPLE 3 Example 2 was repeated except that when the cell weight reached 34 g, propionic acid was fed to the fermenter instead of glucose at a rate of 2.8 g hr-1. EXAMPLE 4 Example 3 was repeated except that feed of propionic acid was commenced when the cell weight reached 39 g. EXAMPLE 5 Example 3 was repeated except that the feed of propionic acid was commenced when the cell weight reached 56 g. EXAMPLE 6 Example 3 was repeated except when the cell weight reached 48 g a single addition of 12 g of propionic acid was made. EXAMPLE Example 2 was repeated except that medium A was used and propionic acid was fed at a rate of 4 g hr 1 instead of glucose throughout the fermentation. MPiE 8 Example 2 was repeated except that when the cell weight reached 38 g, a mixture of glucose and propionic acid was fed to the fermenter, instead of glucose, at a rate of 5.2 g hr-1 glucose and 2.8 g hr-1 propionic acid. EXAMPLE 9 Example 8 was repeated except that the mixture of glucose and propionic acid was fed at a rate of 6.8 g hr glucose and 1.2 g hr propionic acid, commencing when the cell weight reached 28 g. In Examples 2 to 9 the propionic acid was added as a solution containing 400 g 1 1. EXAMPLE 10 Example 2 was repeated except that when the cell weight reached 28 g, isobutyric acid was fed to the fermentation vessel at a rate of 2 g hr 1 in place of glucose. The isobutyric acid was added as a solution containing 150 s 1 1 In Examples 3 - 6 and 8 - 10 the fermentations were continued until the ratio of the weight of acid fed to the fermenter to the sum of the weight of glucose fed to the fermenter after the cell weight had reached 26 g, i.e. when the system became nitrogen depleted, and the weight of acid fed to the fermenter, reached the values set out in Table 1. AMPDE 11 Example 2 was repeated except that when the cell weight reached 26.4 g, 3-chloropropionic acid was fed to the fermenter instead of glucose at a rate of 4 g her 1 for 5 hours. EXA9IE 12 Example 11 was repeated except that feed of 3-chloropropionic acid was commenced when the cell weight reached 34.4 g. EXAMPLE 13 Example 12 was repeated except that a single addition of 4 g of 3-chloropropionic was made when the cell weight reached 30 g and then glucose was fed at a rate of 6.8 g hr for 7 hours. In Examples 11 - 13 the 3-chloropropionic acid was added as a solution containing 50 g 1 EXAMPLE 14 Example 2 was repeated except that when the cell weight reached 31 g, acrylic acid was fed to the fermenter at a rate of 4 g her 1 for 5 hours instead of glucose. The acrylic acid was fed as a solution containing 100 g 1 1. Table 1 EMI19.1 <tb> <SEP> Final <SEP> Amount <SEP> of <tb> <SEP> Acid <SEP> feed <SEP> cell <SEP> Polymer <tb> Example <SEP> Acid <SEP> ratio* <SEP> concentration <SEP> in <SEP> cells. <tb> <SEP> (%) <SEP> (g.1-1) <SEP> (% <SEP> by <SEP> weight) <tb> <SEP> 2 <SEP> none <SEP> 0 <SEP> 20.0 <SEP> 70 <tb> <SEP> 3 <SEP> propionic <SEP> 75 <SEP> 15.6 <SEP> 70 <tb> <SEP> 4 <SEP> propionic <SEP> 50 <SEP> 13.3 <SEP> 60 <tb> <SEP> 5 <SEP> propionic <SEP> 33 <SEP> 16.0 <SEP> 70 <tb> <SEP> 6 <SEP> propionic <SEP> 4 <SEP> 13.0 <SEP> 63 <tb> <SEP> 7 <SEP> propionic <SEP> 100 <SEP> 6.4 <SEP> 55 <tb> <SEP> 8 <SEP> propionic <SEP> 17 <SEP> 13.6 <SEP> 55 <tb> <SEP> 9 <SEP> propionic <SEP> 9-5 <SEP> 14.2 <SEP> 67 <tb> <SEP> 10 <SEP> isobutyric <SEP> 66 <SEP> 13.0 <SEP> 50 <tb> <SEP> ll <SEP> 3-chn <SEP> oropropionic <SEP> 61 <SEP> 7.4 <SEP> 25 <tb> <SEP> 12 <SEP> 3-cbloropropionic <SEP> 33 <SEP> 4.5 <SEP> 20 <tb> <SEP> 13 <SEP> 3-chloropropionic <SEP> 6.5 <SEP> 9.3 <SEP> 35 <tb> <SEP> 14 <SEP> acrylic <SEP> 50 <SEP> 6.0 <SEP> 25 <tb> * acid feed ratio is defined as the weight of acid fed to the fermenter divided by the sum of the weight of glucose added after the cell dry weight reached 26 g and the weight of acid fed to the fermenter, said ratio being expressed as a percentage The amount of comonomer units in the polymers of Examples 2 to 14 was determined (a) by methanolysis and gas chromatography and (b) by 13C nuclear magnetic resonance spectroscopy. The molecular weights of the polymers were determined by gel permeation chromatography. Chlorine analyses were also performed on the polymers of Examples 2, 11, 12 and 13. The results are shown in Table 2. In the table 3 - W indicates 3-hydroxyvalerate units while the units A are those mentioned hereinbefore and which are believed to be 3-hydroxypropionate units. It is seen that little of the chlorine from 3-chloropropionic acid is to be found in the polymer. It would therefore appear that during the metabolism of 3-chloropropionic acid, chlorine is replaced and the resulting product metabolised to give the units A and the 3-hydroxyvalerate units. However the chlorine content of the polymers of Examples 11 - 13 may indicate that some of the chlorine is present as chlorine containing groups R in the units II, possibly as RÚ = chloroethyl and Rê = R = R4 = H, n = 1. Table 2 EMI21.1 Mole <SEP> % <SEP> units <SEP> II <SEP> Molecular <SEP> weight <tb> Example <SEP> Acid <SEP> employed <SEP> Units <SEP> II <SEP> found <SEP> by <SEP> NMR <SEP> by <SEP> methanolysis <SEP> and <SEP> Mw <SEP> x <SEP> 10-3 <SEP> Mw/Mn <SEP> chlorine <tb> <SEP> gas <SEP> chromatogrphy <tb> <SEP> 2 <SEP> none <SEP> none <SEP> 0 <SEP> 0 <SEP> 292 <SEP> 2.75 <SEP> 40 <tb> <SEP> 3 <SEP> propionic <SEP> 3-HV <SEP> 27 <SEP> 33 <SEP> 207 <SEP> 4.23 <tb> <SEP> 4 <SEP> propionic <SEP> 3-HV <SEP> 24 <SEP> 27 <SEP> 374 <SEP> 1.89 <tb> <SEP> 5 <SEP> propionic <SEP> 3-HV <SEP> 13 <SEP> 14 <SEP> 259 <SEP> 3.50 <tb> <SEP> 6 <SEP> propionic <SEP> 3-HV <SEP> 6 <SEP> 3 <SEP> 348 <SEP> 1.66 <tb> <SEP> 7 <SEP> propionic <SEP> 3-HV <SEP> 25 <SEP> 26 <SEP> 336 <SEP> 1.70 <tb> <SEP> 8 <SEP> propionic <SEP> 3-HV <SEP> 15 <SEP> 14 <SEP> 389 <SEP> 1.67 <tb> <SEP> 9 <SEP> propionic <SEP> 3-HV <SEP> 6 <SEP> 7 <SEP> 243 <SEP> 2.56 <tb> 10 <SEP> isobutyric <SEP> 3-HV <SEP> 30 <SEP> 29 <SEP> 274 <SEP> 2.38 <tb> <SEP> 3-HV <SEP> 7 <SEP> 11 <SEP> 3-chloropropionic <SEP> A <SEP> 1.8 <SEP> - <SEP> 383 <SEP> 2.99 <SEP> 475 <tb> <SEP> 3-HV <SEP> 4 <SEP> 12 <SEP> 3-chloropropionic <SEP> A <SEP> 1.2 <SEP> - <SEP> 376 <SEP> 1.77 <SEP> 265 <tb> <SEP> 3-HV <SEP> 2 <SEP> 13 <SEP> 3-chloropropionic <SEP> A <SEP> 0.6 <SEP> - <SEP> 311 <SEP> 1.99 <SEP> 45 <tb> <SEP> 3-HV <SEP> 1 <SEP> 14 <SEP> acrylic <SEP> A <SEP> 6.5 <SEP> - <SEP> 353 <SEP> 2.36 <tb> High resolution 13C NMR was used to investigate the monomer sequences of the copolymers of Examples 3 - 10. The signal derived from the carbon atom of the carbonyl group was found to occur at different chemical shifts depending upon its environment. Thus with polymers containing the units I and II (where n = 1, R1 = C2H5, Rê = R = R4 =H) the possible sequence are A. butyrate - butyrate, i. e. EMI22.1 3. valerate-valerate, i.e. EMI22.2 C. butyrate - valerate, i. e. EMI22.3 NMR examination of the polymers of Examples 2 - 10 revealed three resonances occurring at 169.07, 169.25 and 169.44 ppm respectively. Following the work of M. Iida et al (Macromolecules 11, 1978, p 490) the resonance at 169.07 ppm can be assigned to the butyrate-butyrate sequence A, and that at 169.44 ppm to the valerate-valerate sequence B. 3y inference the signal at 169.25 must arise from the butyrate-valerate sequence C. Quantitative analysis of the INNER results of the copolymer of example 10 gave the following results: sequence A (butyrate-butyrate) 55% sequence 3 (valerate-valerate) 14% sequence C (butyrate-valerate) 31% These results clearly indicate that the polymer of Example 10 contains a substantial amount of a copolymer of units I and II (where n = 1, R1 = C2H5, , Rê= = R4 = H). However it is possible that some homopolymer of repeat unit I is also present. All the polymers of Examples 2 - 14 had the D(-) configuration. The melting behaviours of the copolymers as extracted was first determined by differential scanning calorimetry (DSC) using the Dupont 1090 system with computerised data analysis. DSC was also performed on the samples after compression moulding at 190 C and slow cooling in the press in order to obtain a fully crystallised product. In each case the specimens were heated at 20 C/min in air and +he temperatures at the start (Ts) and peak (Tp) of the melting endotherm, together with its area, were noted. Heating of the annealed sample was continued to 2000C and, after isotherming for one minute to ensure complete melting, it was quenched in liQtiid nitrogen. The DSC was then re-run in order to determine the glass transition temperature (tug) of the amorphous phase. Finally the densities of the annealed copolymers were measured by flotation in a density gradient. The results are shown in Table 3. Table 3 EMI24.1 <SEP> DSC <SEP> on <SEP> extracted <SEP> polymer <SEP> DSC <SEP> on <SEP> annealed <SEP> polymer <tb> <SEP> Example <SEP> 0 <SEP> I <SEP> <SEP> Density <tb> <SEP> Ts C <SEP> Tp C <SEP> Area <SEP> J.g-1 <SEP> Tg C <SEP> Ts C <SEP> Tp C <SEP> Area <SEP> g.cm-3 <tb> <SEP> J.g-1 <tb> <SEP> 2 <SEP> 140 <SEP> 183 <SEP> 100 <SEP> 5,9 <SEP> 140 <SEP> 191 <SEP> 127 <SEP> 1,256 <tb> <SEP> 3 <SEP> 120 <SEP> 125 <SEP> 5 <SEP> -1,9 <SEP> 140 <SEP> 171 <SEP> 18 <SEP> 1,172 <tb> <SEP> 166 <SEP> 20 <tb> <SEP> 4 <SEP> 120 <SEP> 170 <SEP> 50 <SEP> 0,8 <SEP> 140 <SEP> 182 <SEP> 44 <SEP> 1,174 <tb> <SEP> 5 <SEP> 110 <SEP> 120 <SEP> 5 <SEP> 2,2 <SEP> 140 <SEP> 177 <SEP> 45 <SEP> 1,200 <tb> <SEP> 170 <SEP> 50 <tb> <SEP> 6 <SEP> 120 <SEP> 172 <SEP> 100 <SEP> 2,7 <SEP> 120 <SEP> 173 <SEP> 96 <SEP> 1,225 <tb> <SEP> 7 <SEP> 80 <SEP> 132 <SEP> 34 <SEP> 0,4 <SEP> 80 <SEP> 132 <SEP> 40 <SEP> 1,198 <tb> <SEP> 120 <SEP> 6 <tb> 8 <SEP> 110 <SEP> 2,0 <SEP> 140 <SEP> 174 <SEP> 43 <SEP> 1,199 <tb> <SEP> 8 <SEP> 110 <SEP> 166 <SEP> 60 <SEP> 2.0 <SEP> 140 <SEP> 174 <SEP> 43 <SEP> 1.199 <tb> <SEP> 9 <SEP> 110 <SEP> 156 <SEP> 89 <SEP> 4.0 <SEP> 110 <SEP> 163 <SEP> 81 <SEP> 1.210 <tb> <SEP> 65 <SEP> 10 <tb> <SEP> 10 <SEP> 50 <SEP> 120 <SEP> 3 <SEP> -2,0 <SEP> 130 <SEP> 172 <SEP> 26 <SEP> 1,138 <tb> <SEP> 168 <SEP> 25 <tb> <SEP> 11 <SEP> 110 <SEP> 170 <SEP> 57 <SEP> 5.0 <SEP> 120 <SEP> 180 <SEP> 73 <SEP> <SEP> 12 <SEP> 110 <SEP> 177 <SEP> 86 <SEP> 4.1 <SEP> 120 <SEP> 173 <SEP> 1 <SEP> <SEP> 86 <SEP> 1.182 <SEP> <tb> <SEP> 13 <SEP> 100 <SEP> 172 <SEP> 98 <SEP> 5.9 <SEP> 120 <SEP> 171 <SEP> <RTI ID=24.7> 96 <SEP> 1.218 <tb> <SEP> 14 <SEP> 110 <SEP> 172 <SEP> 84 <SEP> 2,7 <SEP> 110 <SEP> 174 <SEP> 75 <SEP> 1.212 <tb> The wide melting ranges of the copolymers indicated that the copolymers were of rather heterogeneous composition. Rowever significant randomisation by ester interchange occurred on annealing since the melting endotherms became much sharper and slightly reduced in area. This is indicative that the polymers are not physical blends of homopolymers but are genuine copolymers. Multiple DSC peaks were observed for the "as extracted" polymers of Examples 3, 5, 8 and 10. The area of the melting endotherms gives an indication of the degree of crystallinity. All the polymers of Examples 3 to 14 after annealing were significantly less crystalline than the control homopolymer of Example 2. EXAMPLE 15 Alcaligenes eutrophus mutant NCIB 11599 was grown by aerobic cultivation at pR 6.8 and 340C in a 5 litre batch fermenter containing 4000 ml of an aqueous medium C which was the same as medium B except that the amount of ammonium sulphate was 5.2 g 1 which is sufficient to support 8.5 g 1 1 of PE-free cells. The substrate was glucose supplied at a rate of 5.5 g 1 hr-1. When the cell concentration reached 7 g 1-1, propionic acid was fed at a rate of 1.58 g 1 1 hr-1 in addition to the glucose. The cells were harvested when the cell dry weight reached 15 g 1 1. The cell suspension was spray dried1 lipids extracted by refluxing the dried cells with methanol1 and the polymer then extracted by reflexing with chloroform. The polymer was recovered by precipitation by adding the chloroform solution to a methanol/water mixture. The copolymer contained 20 mole % of repeat units II where R1 was ethyl, R2, R3, R were each hydrogen, and n = 1. The copolymer had a molecular weight of 350,000, and was insoluble in cold methyl ethyl ketone. When 2 g of the copolymer was refluxed with 100 ml of methyl ethyl ketone for 1 hour it all dis- solved: on cooling the solution a gelatinous mass was formed In contrast less than 0.1 g of a 3-hxdroxybutyrate homopolymer dissolved when 2 g of the homopolymer was refluxed with 100 ml of methyl ethyl ketone. When these solubility tests were repeated with ethanol in place of methyl ethyl ketone, about 0.7 g of the copolymer, and less than 0.04 g of the homopolymer, had dissolved after refluxing for 1 hour. The solubility of the copolymer in ethanol was also assessed at a lower concentration: thus 0.5 g of the copolymer was refluxed with 1 litre of ethanol for 1 hour. Less than 0.2 g of the copolymer dissolved. In contrast it is noted that the polymers described by Wallen et al in "Environmental Science and Technology" 8 (1974) pages 576 - 579 were said to be soluble in hot ethanol. This 16 Aqueous media D, E and F were made up to the following compositions, per litre of deionised water: Medium D (NH4)SO4 12 g MgS04.7R20 1.2 g K2SO4 1.5 g CaCl2 0.12 g FeSO4.7H2O 0.1 g Znso4 7E2 o.oo6 g MnSO4.4H2O 0.006 g CuSO4.5H2O 0.0015 g H2SO4 (concentrated) 1 ml Medium E H3PO4 (1.1 M) 2.4 ml glucose 40 g Medium F Wi-P04 (1.1 M) 2.4 ml propionic acid 40 g A sterilised batch fermenter of nominal capacity 250 litres was filled to the 130 litre mark with a mixture of approximately equal volumes of media D and E. A small sample of the medium in the fermenter was then analysed for nitrogen content. The fermenter was then inoculated with Alcaligenes eutrophus mutant NCIB 11599 and fermentation conducted aerobically at 34 C with automatic pH control at 6.8 by addition of a sodium hydroxide solution. The amount of assimilable nitrogen present in the fermenter was sufficient to support the growth of the micro-organism to only about 1.2 kg of polymer-free cells. When the cell weight reached about 1.05 kg feed of medium E was commenced at a rate of 6.5 litres,Zhour. When the weight of cells reached approximately 1700 g feed of medium E was stopped and feed of medium F commenced at a rate of 6.5 litres/hour, and fermentation continued until about 2. kg of cells had been produced. The cell suspension was then concentrated by centri f,lgation to a concentration of about 60 g/litre and the polymer extracted therefrom by contacting 1 volume of the suspension with 2 volumes of 1,2-dichloroethane (DCE) In a Silvers mixer at 200C for 15 minutes. The DCE phase was separated from the aqueous phase, hich contained the cell debris, and filtered. The polymer was precipitated by adding 1 volume of the filtered DCE phase to 4 volumes of a methanol/water mixture (4 volumes of methanol to I volume of water). The precipitated polymer was collected by filtraton, washed with methanol, and dried in an oven for 4 hours at 100 C. The polymer had a melting range, as determined by differential scanning calorimetry, of about 100 C to 180 C with a peak in the melting endotherm at 168 C. EXAMPLE 17 The fermentation procedure of Example 16 was repeated except that the changeover from feeding medium E to feeding medium F took place when the weight of cells was approximately 3.5 kg. The medium F was fed at a rate of 11.4 litres/hour for 4 hours and then reduced to 3.2 litres/hour and maintained at this level for a further 9 hours at which stage the weight of cells was about 3.9 kg. In this example the amount of assimilable nitrogen present in the fermenter was sufficient to support the growth of the micro-organism to only about 1.5 kg of polymer-free cells. The cell suspension was concentrated by centrifugation and then the polymer was extracted from the concentrated cell suspension by the procedure described in Example 15. EXAMPLE 18 A 250 litre fermenter was charged and inoculated as in Example 16. The amount of assimilable nitrogen was sufficient to support growth of the micro-organisms only to about 1.9 kg of polymer-free cells. As in Example 16 fermentation was conducted aerobically at 340C at a pH of 6.8. When the cell weight reached about 1.0 kg feeds of medium E and of a medium G at rates of a.7 itres/hour and 4.6 litres/hour respectively were commenced and continued until the cell weight reached about 3.9 kg. medium - had the composition, er - tre of deionised water: H3PO4 (1.1 M) 1.2 ml propionic acid 20 g The cell suspension was concentrated by centrifugation and then the polymer extracted from ne concentrated cell suspension by the procedure described in Example . EXAMPLE 19 The procedure of Example 17 was repeated on a larger scale using a fermenter of nominal capacity 1000 litres which was filled to the 500 litre mark with approximately equal volumes of media D and E. In this example the feed of medium E was commenced, at a rate of 25 litres/hour, when the weight of cells was about 4 kg and the feed of medium F was commenced, at a rate of 37.5 litres/hour, when the weight of the cells was about 8 kg. The feeds of media E and F were continued until the weight of cells was about 10 kg. The amount of assimilable nitrogen present was sufficient to support the growth of the micro-organism to only about 4.1 kg of polymer-free cells. EXAMPLE 20 Example 19 was repeated except that the feed rate of medium F was 25 litres/hour and the fermentation was continued until the weight of the cells was about ll kg. In this case the amount of assimilable nitrogen present was sufficient to support the growth of the micro-organism to only about 4 kg of polymerfree cells. The polymers of Examples 16 - 20 were each copolymers containing 3.hydroxybutyrate (HB) units and 3-hydroxyvalerate (HV) units1 and had weight average molecular weights above 300,000. They each had the D(-) configuration. 100 parts by weight of each of the polymers of Examples 16 - 20, and of a 3-hydroxybutyrate homopoly:n.er were slurried with about 10 parts by weight of chloroform and 1 part by weight of Steamic talc, and granulated at room temperature through a domestic mincer. The compositions were then dried to remove the chloroform and extruded at 1900C and regranulated. The resulting granules were injection moulded at 185 C into test bars using a mould temperature of 600C and a cooling time of 20 sec. The tensile properties were measured according to ASTM D 638-77a at a rate of 50 mm/min and the impact strength assessed by the Izod impact test according to ASTM D 256-78. The results are shown in Table 4. Table 4 EMI29.1 <tb> <SEP> HV/!B <SEP> molar <SEP> Izod <SEP> Impact <SEP> strength <tb> Example <SEP> ratio <SEP> odulus* <SEP> Tensile <SEP> Extension <SEP> (U/m) <SEP> <tb> <SEP> # <SEP> (GPa) <SEP> <SEP> Strength <SEP> to <SEP> Break <tb> <SEP> by <SEP> GC <SEP> by <SEP> NMR <SEP> (MPa) <SEP> (X) <SEP> <SEP> 1 <SEP> mm <SEP> notch <SEP> unnotched <SEP> <tb> <SEP> 16 <SEP> 18/82 <SEP> 20/80 <SEP> 1.47 <SEP> 25 <SEP> 10-31 <SEP> 66 <SEP> 463 <SEP> <tb> <SEP> 17 <SEP> 4/96 <SEP> 6/94 <SEP> 2.98 <SEP> 33 <SEP> 5-7 <SEP> 23 <SEP> 140 <SEP> <tb> <SEP> 18 <SEP> 8/92 <SEP> 7/93 <SEP> 2.10 <SEP> 31 <SEP> 14-19 <SEP> 106 <SEP> 408 <SEP> <tb> <SEP> 19 <SEP> 1/99 <SEP> 4/96 <SEP> 2.70 <SEP> 35 <SEP> 8-14 <SEP> 56 <SEP> 191 <SEP> <tb> <SEP> 20 <SEP> 4/96 <SEP> 4/96 <SEP> 2.48 <SEP> 35 <SEP> 8-15 <SEP> 23 <SEP> 140 <tb> <SEP> homo <tb> polymer <SEP> 0/10C <SEP> 0/100 <SEP> 3.25 <SEP> 40 <SEP> 6-13 <SEP> 65 <SEP> 115 <tb> * at 0.5% extension EXAMPLE 21 A PVC formulation was made by dry blending the following ingredients at room temperature: : parts by weight (i) vinyl chloride homopolymer (K62) 100 (ii) a complex tin thiooctyl stabiliser based on a di-N-dithioglycollic acid ester 1.5 (iii) methyl methacryrate/butadiene/ warts by weight styrene PVC impact modifier 8 (iv) wax (external lubricant) 0.8 (v) glyceryl monoester (internal lubricant) (vi) HB polymer (processing aid) 2 The RB polymer processing aids were a) a 3-hydroxybutyrate homopolymer prepared by the procedure of Example 2 b) the copolymer of Example 7 (copolymer A) c) the copolymer of Example 16 (copolymer B) The processing aids were slurried with about 10% by weight of chloroform, granulated at room temperature through a domestic mincer, dried, melt extruded at 190 C, regranulated, and ground to a particle size below 150 m before incorporation into the PVC dry blend. The dry blends were tested as follows: 1. 50 g of the mixture was poured into the mixing head of a Brabender Plastograph maintained at 1800C rotating at 18 rpm under a pressure ram loaded with a 5 kg weight. The time taken for gelation to occur was measured. 2. The mixture was cold compressed to form a candle which was then charged to an extrusion rheometer maintained at 170 0C and fitted with a die having a circular orifice of 1 mm diameter and 20 mm land length. After the charge had heated to 170 C, it was extruded at '-creasing rates. The appearance of the extrudate was noted and the melt extensibility assessed by attempting to draw the extrudate away from the die. The results are shown in Table 5. Table 5 EMI31.1 <tb> <SEP> Extrusion <SEP> at <SEP> 170 C <tb> Processing <SEP> aid <SEP> Gelation <SEP> time <SEP> Melt <tb> <SEP> min) <SEP> at <SEP> 180 C <SEP> Appearance <SEP> extensibility <tb> None <SEP> 12 <SEP> Severe <SEP> sharkskin <SEP> at <SEP> low <SEP> Poor <tb> <SEP> extrusion <SEP> rates; ; <tb> <SEP> ripple <SEP> at <SEP> higher <SEP> rates <tb> homopolymer <SEP> 9.5 <SEP> Poor <SEP> with <SEP> a <SEP> lot <SEP> of <SEP> Poor <tb> <SEP> unmelted <SEP> polymer <SEP> <tb> <SEP> clearly <SEP> visible <tb> copolymer <SEP> A <SEP> 1.0 <SEP> Excellent <SEP> - <SEP> very <SEP> smooth <SEP> Good <tb> copolymer <SEP> 3 <SEP> 1.5 <SEP> Smooth, <SEP> but <SEP> occasional <SEP> Fair <tb> <SEP> unmelted <SEP> particles <tb> This example shows that the copolymers are superior to 3-hydroxybutyrate homopolymer as a vinyl chloride polymer processing aid. The more random copolymer A was clearly superior to the copolymer 3. EXAMPLE 22 A medium H was made up to the following composition: (NH4) 2S04 lg KH2PO4 2g (Na)2HPO4 3g MgSO4.7H2O 0.2g CaCl2 0.01g FeS04.7H20 0.005 g MnSO4.4H2O 0.002g NaCO3.10H2O 0.1g (NH2)2 CO 1.5 g deionised water - to 1 litre This medium had a pH of 7. Eight 1 litre shake flasks each containing 500 ml of medium E, in which 0.5 g of methacrylic acid had been dissolved, were each inoculated with 5 ml of a starter culture of Nocardia salmonicolor strain ATCC 19149 and incubated at 3200 on a gyratory shaker. 0.5 G of methacrylic acid was added to each flask at intervals of 24, 48 and 72 hours after inoculation, and a final addition of 0.25 G of methacrylic acid was made 96 hours after inoculfation. After a total of 108 hours after inoculation the flasks were examined: little or no growth of the microorganism was apparent in any of the flasks. The contents of the flasks were combined and centrifuged to give a pellet of cells which was dried in an oven and weighed. The weight of the pellet was 2.81 g. The cell content of the inoculum was also determined and found to be 69.75 g.ll: Hence the total weight of cells added, as the inoculun, to the flasks was 2.79 g. It is concluded that, at the concentrations of methacrylic acid employed, this strain does not assimilate methacrylic acid. EXAMPLES 23 In these examples a range of acids and derivatives thereof were screened for their ability to give copolymers. The technique employed was as follows: Alcaligenes eutroohus mutant NOIB 11599 was grown by aerobic cultivation at pH 6.6 and 3400 in a 5 litre batch fermenter containing 3500 to 4000 ml of an aqueous medium having the composition, per litre of de-ionised water: glucose 17 g (NH4)2SO4 4 g MgSO4.7H2O 0.8 g H3PO4(1.1 M) 12 ml FeSO.7H2O 15 mg Trace element solution (as used in Example 1) 36 ml The pH was controlled at 6.8 by the automatic addition of a 9:1 v/v mixtures of 4 M potassium hydroxide and 4 M sodium hydroxide. This addition of a KOH/NaOH mixture to control pR also served to supply potassium and sodium to the cultivation medium. The amount of assimilable nitrogen (provided by the 4 g/l of ammonium sulphate) was sufficient to support only about 6.5 gel of HB polymer-free cells. As about 16 g/i of glucose are required 5 produce 6.5 g/l of HB polyester Tree cells, the mount of glucose present was sufficient to provide a small carbon excess. 3y monitoring the residual glucose concentration and also the dissolved oxygen tension trace, a clear indication was obtained when the system became starved of assimilable nitrogen. At this stage feed of the comonocer component, i.e. the acid or derivative under study, was commenced and continued until a total of about 0.1 to 5 g/l of the comonomer component had been added. After completin of the addition of the comonomer com- ponent, the cells were harvested by centrifugation. A sample of the centrifuged cells was freeze dried and the polymer was extracted with chloroform. The polymer was analysed by a gas chromatography/ mass spectroscopy (GCMS) technique (which involves a preliminary transesterification step) or by nuclear magnetic resonance spectros- copy (NMR). The results are shown in table 6. In this table the T results are quoted where possible as these are considered to be the more definitive since the NNR technique does not involve a transesterification step. TABLE 6 EMI34.1 <tb> <SEP> Comonomer <SEP> component <tb> Example <SEP> period <SEP> over <SEP> Units <SEP> II <SEP> found <tb> <SEP> which <SEP> comonomer <SEP> total <SEP> amount <tb> <SEP> nature <SEP> component <SEP> added <SEP> added <tb> <SEP> (hr) <SEP> (g/l) <tb> <SEP> 23 <SEP> propionic <SEP> acid <SEP> 5 <SEP> 5 <SEP> 3-HV <tb> <SEP> 24 <SEP> methyl <SEP> propionate <SEP> 1.75 <SEP> 2.5 <tb> <SEP> 25 <SEP> 3-hydroxypropionic <SEP> 1 <SEP> 1 <SEP> 3-HV <SEP> and <SEP> "A" <tb> <SEP> acid <tb> <SEP> 26 <SEP> 3-hydroxypropionic <SEP> 16,5 <SEP> 3,4 <SEP> 3-HV <SEP> and <SEP> "A" <tb> <SEP> acid <tb> <SEP> 27 <SEP> 2-chloropropionic <SEP> 0,5 <SEP> 0.3 <SEP> none <tb> <SEP> acid <tb> <SEP> 28 <SEP> 3-chloropropionic <SEP> 0,5 <SEP> 0,3 <SEP> 3-HV <SEP> and <SEP> "A" <tb> <SEP> acid <tb> <SEP> 29 <SEP> 3-ethoxypropionic <SEP> 1 <SEP> 1 <tb> <SEP> acid <tb> <SEP> 30 <SEP> isobutyric <SEP> acid <SEP> 5 <SEP> 5 <tb> <SEP> 31 <SEP> 2-chlorobutyrìc <SEP> 0.5 <SEP> 0,3 <SEP> none <tb> <SEP> acid <tb> <SEP> 32 <SEP> 2-hydroxybutyric <SEP> 0.5 <SEP> 1 <tb> <SEP> acid <tb> <SEP> 33 <SEP> 4-hydroxybutyric <SEP> 3 <SEP> 2 <SEP> none <tb> <SEP> acid <tb> <SEP> 34 <SEP> phenyl <SEP> acetic <SEP> acid <SEP> 2 <SEP> 2 <SEP> none <tb> <SEP> 35 <SEP> 2-chloroacetic <SEP> 305 <SEP> 0,3 <SEP> none <tb> <SEP> acid <tb> <SEP> 36 <SEP> acrylic <SEP> acid <SEP> 5 <SEP> 3.5 <SEP> 3-HV <SEP> and <SEP> "s" <SEP> <tb> <SEP> 37 <SEP> 2-methylacrylic <SEP> 0.5 <SEP> 1 <SEP> none <tb> <SEP> acid <tb> gable 6 (cont'd) EMI35.1 <tb> <SEP> Comonomer <SEP> component <tb> Example <SEP> period <SEP> over <SEP> Units <SEP> II <SEP> found <tb> <SEP> which <SEP> comonomer <SEP> total <SEP> amound <tb> <SEP> nature <SEP> component <SEP> added <SEP> added <tb> <SEP> (hr) <SEP> (g/l) <tb> <SEP> 38 <SEP> 2,3-dimethyl- <SEP> 0.5 <SEP> 1 <SEP> none <tb> <SEP> acrylic <SEP> acid <tb> <SEP> 39 <SEP> 3,3-dimethyl- <SEP> 1 <SEP> 1 <SEP> none <tb> <SEP> acrylic <SEP> acid <tb> <SEP> 40 <SEP> glyoxylic <SEP> 5 <SEP> 2 <SEP> none <tb> <SEP> 41 <SEP> glycolic <SEP> 3 <SEP> 2 <SEP> none <tb> <SEP> 42 <SEP> lactic <SEP> 5.5 <SEP> 3 <SEP> none* <tb> <SEP> 43 <SEP> benzoic <SEP> 24 <SEP> 5 <SEP> none* <tb> <SEP> 44 <SEP> formic <SEP> 4 <SEP> 2 <SEP> none <tb> <SEP> 45 <SEP> valeric <SEP> 4 <SEP> 2.5 <SEP> 3-HV*+ <tb> * Only GCMS analysis performed. + polymer contained about 3 35 units. Similar results were obtained when Examples 23-44 were repeated using an alternative cultivation regime wherein a mixture of glucose and the comonomer component was continuously added after nitrogen starvation had been attained. EXAMPLE 46-51 Alcaligenes eutrophus mutant NCIB 11599 was grown by continuous aerobic cultivation at pH 6.8 and 340C in a 5 litre fermenter with a working volume of about 4 litres at a dilution ratio (reciprocal of residence time) of 0.085 hr-1. . The aqueous medium employed had the following composition, per litre of deionised water: MgSO4.7H2O 0.8 g K2SO4 0.45 g NaSO4 0.05 g E3P04( 1-1 M) 12 ml Trace element solution (as used in Example 1) 36 ml The ingredients set out in Table 7 were also continuously supplied to the fermenter TABLE 7 EMI36.1 <tb> <SEP> Amount <SEP> added <SEP> per <SEP> Supplied <SEP> as <SEP> a <SEP> solution <tb> <SEP> Ingredient <SEP> litre <SEP> of <SEP> medium <SEP> containing, <SEP> per <SEP> llitre <tb> <SEP> in <SEP> the <SEP> fermenter <SEP> of <SEP> deionised <SEP> water <tb> <SEP> Fe <SEP> 5 <SEP> mg <SEP> (2g <SEP> FeSO4.7H2O <tb> <SEP> (1 <SEP> ml <SEP> conc. <SEP> H2SO4. <tb> <SEP> N <SEP> 850 <SEP> mg <SEP> 50 <SEP> g <SEP> NH4OH <tb> propoionic <SEP> acid <SEP> 26 <SEP> g <SEP> 400 <SEP> g <SEP> propionic <SEP> acid <tb> carylic <SEP> acid <SEP> see <SEP> Table <SEP> 8 <SEP> 40 <SEP> g <SEP> acrylic <SEP> acid <tb> The amount of nitrogen was sufficient to support only 6.5 g/l of HB-polymer free cells. pH was controlled at 6.8 by the automatic addition of a 9:1 v/v mixture of 4 M potassium hydroxide and 4 M sodium hydroxide. Fermentation was commenced using propionic acrid as the sole substrate. 3 days after steady state conditions had been achieved a sample of the aqueous cell suspension product was taken and then acrylic acid was added as a cosubstrate in increasing amounts, Prior to each increase in acrylic acid concentration a sample of the aqueous cell suspension product was taken. Ferment- ation under steady state conditions for at least 3 days was performed at each acrylic acid concentration before taking each sample. Each sample of the aqueous cell suspension product was centrifuged to harvest the cells which were then freeze dried0 The polymer was extracted from the cells with chloroform and then analysed by NMRo The results are shown in Table 8. TABLE 8 EMI37.1 <tb> <SEP> Amount <SEP> of <tb> <SEP> acrylic <SEP> acid <tb> <SEP> added, <SEP> per <SEP> Cell <SEP> dry <SEP> polymer <SEP> content <tb> Example <SEP> litre <SEP> of <SEP> <SEP> weight <SEP> (;J0 <SEP> by <SEP> weight) <SEP> NMR <SEP> analysis <SEP> <tb> <SEP> medium <SEP> in <SEP> g/l <SEP> (mole%) <tb> <SEP> the <SEP> fermenter <tb> <SEP> (g) <SEP> 3-HB <SEP> 3-HV <SEP> other <tb> <SEP> 46 <SEP> 0 <SEP> 11,9 <SEP> 45 <SEP> 70 <SEP> 30 <SEP> 0 <tb> <SEP> 47 <SEP> 0.25 <SEP> 11.8 <SEP> 45 <SEP> 70 <SEP> 19 <SEP> 11 <SEP> (units <SEP> A) <SEP> <tb> <SEP> 48 <SEP> 0.5 <SEP> 10.8 <SEP> 40 <SEP> 72 <SEP> 20 <SEP> 5 <SEP> 8 <SEP> (units <SEP> A) <SEP> <tb> <SEP> 49 <SEP> 1.0 <SEP> 10.5 <SEP> 38 <SEP> 81 <SEP> 19 <SEP> 0 <tb> <SEP> 50 <SEP> 2.0 <SEP> 9.3 <SEP> 30 <SEP> 90 <SEP> 10 <SEP> 0 <tb> <SEP> 51 <SEP> 4.5 <SEP> 7.1 <SEP> 9 <SEP> 89 <SEP> 11 <SEP> 0 <tb> EXAMPLE 52 In this example Alcaligenes eutrophus mutant NCIB 11599 is grown aerobically at 340C in under phosphorus, rather than nitrogen, limitation, A 5 litre fermenter was charged with 3 litres of an aqueous medium contanning, per litre of deionised water: (NH4)2SO4 6.2 g H3PO(1.1 M) 1.5 ml MgSO4.7H2O 0.8 FeSO4.7H2O 15 mg Trace element solution (as used in Example 1) 36 ml Fermentation was controlled at pH 6.8 automatically by the addition of a 9:1 v/v mirture of 4 M KOH and 4 M NaOH. The amount of phosphorus was sufficient to support only about 8 g/l of RB polymer free cells. The fermenter was inoculated with a 48 hr shake flask culture and then 5 ,4 of glucose was added. When all the glucose had been utilised (at which stage the cell concentration was about 2.5 g/l), Dropionic acid was added, as a solution containing 300 g/l propionic acid) at a rate of 0.8 g/l/hr for 54 hours. The cells were then harvested, the polymer extracted with chloroform, and analysed by C4S. The results were as follows: Final cell concentration 20 g/l Polymer content 60% mole % 3-HV 40% EXAMPLES 53-57 In these Examples two strains of Nocardia salmonicolor were grown on glucose and then polymer accumulation induced using various acids. In each exanple a 250 ml shake flask was charged with 50 ml of an aqueous medium containing, per litre of deionised water glucose ' 10 g K2HPO4 1.9 g N2NPO4 1056 g (NH4)2SO4 1.8 g MgSO4.7H2O 0.2 g FeCl3.6H2O 0.001 g Trace element solution (as used in Example 1) 1 ml The pH of the aqueous medium was 7.0. The flask was inoculated with the organism and grown, with gyratory shaking, at 30 C for 24 hours. The resultant suspension was then centnfuged and the supernatant aqueous medium discarded, The centrifuged pellet was then resuspended in 50 ml of an aqueous medium identical to that above except that the 10 g,4 of glucose was replaced by 1 g/l of the acid and the 1.8 g/l of ammonium sulphate was omitted, The resuspended cells were shaken for a further 24 hours at 300C and then the cell suspension centrifuged. The centrifuged pellet of cells was washed twice with methanol and analysed for polymer content. The polymer was analysed by GCMS. The results are shown in Table 9. TABLE 9 EMI39.1 <tb> <SEP> GCMS <tb> Example <SEP> N. <SEP> salmonicolor <SEP> acid <SEP> approx. <SEP> mole <SEP> % <tb> <SEP> strain <SEP> polymer <SEP> content <tb> <SEP> (% <SEP> by <SEP> weight) <SEP> 3-HB <SEP> 3-HV <tb> <SEP> 53 <SEP> ATCC <SEP> 19149 <SEP> isobutyric <SEP> 10 <SEP> 35 <SEP> 65 <tb> <SEP> 54 <SEP> ATCC <SEP> 19149 <SEP> 2-chloropropionic <SEP> 12 <SEP> 63 <SEP> 37 <tb> <SEP> 55 <SEP> ATCC <SEP> 21243 <SEP> propionic <SEP> 10 <SEP> 38 <SEP> 72 <tb> <SEP> 56 <SEP> ATCC <SEP> 21243 <SEP> isobutyric <SEP> 10 <SEP> 30 <SEP> 70 <tb> <SEP> 57 <SEP> ATCC <SEP> 21243 <SEP> 2-chloropropionic <SEP> 12 <SEP> 38 <SEP> 62 <tb>

Claims: 1. A process for the production of a themoplastic polyester were a micro-organism that is capable of accumulating a polyester is cultivated in an aqueous medium on a water soluble assimilable carbon containing substrate with at least part of the cultivation beg conducted under conditions causing polyester to be accumulated by the micro-organism, characterised in that, at least during part of the period when polyester is being accumulated, the substrate comprises an organic acid, or derivative thereof, that is metabolisable by said micro-organism under said polyester accumulating con ditions to a polyester other than one composed solely of -0.CH(CH3).CH2.CO- repeat units, the amount of combined carbon in said acid, or derivative thereof, constituting at least 2% by weight of the total combined carbon in the substrate present through- out said period. 2. A process according to claim 1 wherein the acid is selected from propionic, isobutyric, and acrylic acid. 3. A process according to claim 1 or claim 2 wherein the acid is the sole substrate for at least part of the period during which the cultivation of the micro-organism is conducted under con editions causing polyester to be accumulated. t. A process according to claim 3 wherein the acid is the sole substrate throughout the cultivation of the micro-organism. 5. A process according to any one of claims 1 to 3 wherein the micro-org-nism is grown using a carbohydrate as a substrate. 6. A process according to claim 5 wherein the carbohydrate is glucose. 7. A process according to claim 5 or claim 6 wherein, for at least part of the period when the cultivation is under conditions causing polyester accumulation, the substrate is a mixture of the acid and the carbohydrate. 8. A process according to any one of claims 1 to 7 wherein the micro-organism is caused to accumulate polyester by cultivation under conditions of limitation of one or more of the essential re quirements for microbial growth but not polyester accumulation. 9. A process according to claim 8 wherein the essential requirement for growth, but not polyester accumulation, that is limited, is the nitrogen source, or the phosphorus source. 10. A process according to any one of claims 1 to 9 wherein the acid, or derivative thereof, that is metabolisable to a polyester other than one composed solely of -O.CH(CH3).CH2.CO- units, is the sole organic acid, or derivative thereof, present during the period when polyester is being accumulated. 11. Copolymers having a weight average molecular weight Dove 10,000 and containing repeat units I -O.CH(CH3).CH2.COand repeat units II -O.CRÚRê.(CR R4)n.COwhere n is O or an integer and RÚ, Rê, R , and R4 are each selected from hydrocarbon radicals; halo- and hydroxy- substituted hydro- carbon radicals; hydroxy radicals; halogen atoms; and hydrogen atoms, provided that, where n is 1 and R2, R3, and R4 are eacn hydrogen atoms, R1 is not methyl and not all of the groups RÚ are ethyl; said repeat units II constituting 0.1 to 50 mol % of the total repeat units in said copolymer. 12. Copolymers having a weight average molecular weight above 10,000 and containing at least 50 mcl % of repeat units -O.CH(CH3).CH2.COand being characterised by a triplet at 4.3 ppm as measured by proton nuclear magnetic resonance spectroscopy and peaks at 59,59 and 33.83 ppm (relative to a tetramethyl silane standard) measured by 13C nuclear magnetic resonance spectroscopy. 13. Copolymers having a weight average molecular weight above 10,000 and containing (a) -O.CH(CH3).CH2.CO- repeat units and (b) -O.CH.2CH2.CO- repeat units alone or in conjunction with -O.CH(C2H5).CH2.CO- repeat units, said units (b) constituting 0.1 to 50 mol % of the total repeat units in said copolymer0

Inventors: COLLINS, STEPHEN HUGH, HOLMES, PAUL ARTHUR, WRIGHT, LEONARD FREDERICK
Applicants: IMPERIAL CHEMICAL INDUSTRIES PLC
Application year: 1982
Main classification: C12P7
IPC codes: C12R1, C08G63, C12P7