ANTIHYPERTENSIVE AGENTS

Abstract: Chromone derivatives such as 7-(3-n-propylamino-2-­ hydroxypropoxy)-2,3-diphenylchromone which are useful as antihypertensive agents.

Description: Antihypertensive Agents Background of the Invention This invention relates to chromone derivatives substituted by 3-amino-2-hydroxypropoxy side chains, medicinal preparations containing the same, and the utilization of such preparations as antihypertensive agents in animals. Compounds related to propranolol, a potent beta-blocker, constitute a large class of compounds which have profound effects on the cardiovascular system and have found utility as antihypertensive, antidysrhythmic and antianginal drugs. However, the beta-blocking properties of these compounds are often undesirable, especially in patients with coronary insufficiencies and bronchial diseases. A compound devoid of beta-blocking effect but retaining antihypertensive effect on the blood pressure of warm-blooded animals has long been sought. Several patents disclose various compounds which either have beta-blocking properties or do not have antihypertensive effects. U. S. Patent No. 3,891,651 discloses compounds which are amides and, it is thought, the nitrogen of the amide which is contained in the isoquinoline fragment is likely to be responsible for any activity in that compound. U. S. Patent No. 3,816,470 discloses various salts of secondary amines with chromone-2-carboxylic acids. U. S. Patent No. 3,812,156 discloses a method of preparing ethyl flavone7-oxyacetate. U. S. Patent No. 3,352,754 discloses simple 7-hydroxy or 7-alkoxy isoflavones which are not amines and which are used for various inflammatory disorders. U. S. Patent No. 3,219,531 discloses 5,7dioxyacetic acid flavone compounds, but no amine functions are present. U. S. Patent No. 3,046,275 discloses 7-dialkylaminoalkoxy derivatives but does not contain any of the hydroxyl groups of the side chain which is central for activity. Various monodialkylaminoethyl ethers of quercetin are disclosed in U. S. Patent No. 2,861,992 but do not contain 3-amino-2hydroxypropoxy side chains. Also not containing that side chain are the compounds disclosed in U. S. Patent No. 2,897,211. Summary of the Invention It has now been found that a marked reduction in the blood pressure of warm-blooded animals can be achieved, without beta-blocking effects in the beta-adrenergic nervous system, by administering to the animal, in an amount effective to reduce hypertension, a chromone of the formula: EMI2.1 or a pharmaceutically acceptable acid addition salt thereof where R1NHCH2-CH(OH)-CH2O- can be in the 6 or 7 position of the chromone nucleus and where R1 is hydrogen, primary or secondary alkyl of 1 to 6 and preferably 1 to 4 carbons, or cycloalkyl or 3 to 6 carbons, or alkene from 2-6 carbon atoms: R2 is hydrogen, lower alkyl, CF3, phenyl, ortho halophenyl or para halophenyl; and R3 is H or phenyl, or benzyl and R4 is H or OH; with the proviso that at least one of R2 or R3 must be phenyl (or one of the halophenyls). It has further been discovered that chromones of formula (1) above where R1NHCH2-CH(OH)-CH2O is in the 7 position of the chromone nucleus, R1 is i-ropyl, n-propyl or cyalopropyl, R2 is phenyl, R3 is phenyl or hydrogen (with the proviso that R1 is cyclopropyl or n-propyl when R3 is hydrogen) and R4 is H or OH have unexpectedly superior antihypertensive properties, especially in terms of being able to achieve long-term activity (e.g., 24 hours) at relatively low dosages. The compounds are usually mixed with a pharmaceutical carrier so that the composition for commercial use contains 0.5 to 20% by weight of the compound. The compositions are normally adapted for peroral or parenteral use, but may be used in other forms such as suppositories. The peroral 'compositions are preferably in the form of tablets, capsules or suspensions, while the parenteral composition is preferably an injectable solution or suspension. Examples of suitable inert pharmaceutical carriers are celluloses (particularly microcrystalline celluloses), sugar syrups, potato starch, talcum, polyethylene glycols and lactose. Examples of suitable acids for forming the acid addition salts are maleic acid, hydrochloric acid, sulfuric acid, phosphoric acid, methanesulfonic acid, tartaric acid, citric acid, and the cation exchange resins such as the carboxylic acid, phosphonic acid and sulfonic acid resins. For sustained release, a coated complex of the compound absorbed onto an ion exchange resin may be employed in accordance with the teaching of British Patent 1,544,761. The usual peroral dosage of the compound is 0.1 to 50 mg. per day (preferably 0.1 to 50 mg.j while the parenteral dosage is normally 0.1 to 40 mg. per day (preferably 0.1 to 10 mg.). The capsules, tablets, syrups and suspensions. of the compounds are prepared by conventional procedures. It should be noted that the compounds of this invention are antihypertensive agents, not hypotensive agents, i.e., they reduce the blood pressure to normal but not below normal. Description of the Invention The compounds of formula (1) above can be prepared by reacting epichlorohydrin or epibromohydrin with a compound of the formula EMI4.1 wherein R3 is the same as defined above and ORg represents a hydroxyl group substituted on the 7 position, in the presence of a solvent and a base to give a product of formula (2) where OR5 is EMI4.2 This epoxide is allowed to react with an amine, R1NH2 or R1R6NH where Ri is as above defined and R6 is hydrogen or benzyl, in an alcoholic solvent at elevated temperature to afford a product of formula (1). When R6 is benzyl, it can be removed by catalytic hydrogenation to give the secondary amine. P. Da Re et al, 3. Med. Chem., Vol. 15, 868-869 (1972), describe the testing of chromones as in formula (1), but where R3 is methyl, for betaXadrenergic blocking activity. Da Re et al. found that all the compounds were devoid of beta-blocking activity, suggesting that chromones would not be expected to have antihypertensive properties. Additionally, ethanolamine anologues of the Da Re et al. materials are disclosed in Vol 15. pages 198-199 of the 3. Med. Chem. (1972). These analogues are beta-blockers, typical of the pronethalol type which owe their activity to the 2-isopropylaminoethanol side chain. Typical 3-amino-2-hydroxypropoxy side chain furochromone compounds are disclosed in papers presented in Drugs of the Future, Vol. III, No. 8 (1978), pages 596-571; Drugs of the Future, Vol.111, No. 11, (1978), pages 816-818; and Therapie, (1977), Vol. 32, pages 111-120. None of these references, of course, even suggest that antihypertensive activity may be possible with or without beta-blocking. None of these references discloses the compounds of this invention. The following general procedures are used in the examples to follow: A. For Epoxides - Epichlorohydrin or epibromohydrin (greater than 2 equivalents) is added in the presence or absence of nitrogen to a stirred solution or suspension of the hydroxy chromone derivative in solvents, such as acetone, aqueous ethanol, 50% aqueous dimethylsulfoxide (DMSO) or water, containing a suitable base, such as potassium carbonate or sodium hydroxide, with or without sodium iodide. The reaction is allowed to proceed either at room temperature or elevated temeprature and monitored by thin layer chromatography (tlc). Product formed as solid is then collected and washed with water. The mother liquor is diluted with water and extracted with chloroform (CHC13) to give more product. Where the product is soluble in solvents, the reaction mixture is filtered off and the solids washed with the solvent. The filtrate is evaporated to give a solid which is washed with water to get rid of remaining traces of the base. Yield is in the 60 to 80% range. This material is usually used directly for further reaction without purification B. For Epoxide Ring-Opening With Amine and the Amine Salt Formation - A white suspension of the chromone epoxide, amine, such as low (large excess) and high (10% excess) boiling point amines, and alcoholic solvent, methanol (A.R.) ethanol (abs.) or isopropanol, is heated at elevated temperatures until the starting material is gone (as followed by tlc.) Since the reaction product is usually soluble in the alcohol, the precipitate is filtered off and the filtrate is evaporated to give a viscous liquid which, upon addition of anhydrous ether or alcohol, crystallizes out. When the product is insoluble in alcohol at the end of the reaction, it is collected, Purification of the amine derivative is performed via acid-base work-up, column chromatography or recrystallization. The amine obtained is either suspended or dissolved in an alcohol, such as MeOH or abs. ethanol (EtOH) or isopropanol, and then acidified with alcoholic solution saturated with hydrogen chloride or another suitable acid. The salt formed is precipitated out by addition of anhydrous either. The salt is then recrystallized from suitable solvents. Example 1 - Preparation of 7-(2-hydroxy-3-isopropylamino propoxy) -2, 3-diphenylchromone hydrochloride: 7- (2 ,3-Epoxypropoxy) -2 ,3-diphenylchromone -To a solution of 50% aqueous DMSO (280 ml) containing sodium hydroxide (5.60 g, 140 mmol) was added 7-hydroxy-2,3diphenylchromone (44.01 g, 140 mmol) and the resultant mixture was stirred until most of the solid went into the solution. Epichlorohydrin was then introduced into the suspension. The solution was stirred at room temperature (RT) for 2.5 days and solid formed during the reaction was collected from time to time (about twice a day). The white solid (total= 47.58 g) was recrystallized from isopropyl alcohol several times to afford 28.15 g of relatively pure white prisms, m.p. 143-145 ; yield 54%. The mother liquor was concentrated to produce more crystals. 7- (2-Hydroxy-3isopropylaminopropoxy) -2, 3-diphenyl- chromone hydrochloride - A white suspension of relatively pure 7-(2,3-epoxypropoxy)-2,3-diphenylchromone (27.78 g, 75 mmol), isopropylamine (18.8 ml), and methanol (AR., 150 ml) was heated under nitrogen at 550C until complete disappearance of the starting epoxide. The white precipitate (1.09 g) in the cooled reaction mixture was filtered off, and the filtrate was evaporated to a golden syrup. Anhydrous ether was added, and white crystals (28.07 g) were obtained. It was column-chromatographed over silica gel. This purification yielded 24.05 g of white crystals, m.p. 152-1540 (75%). The amine thus obtained was dissolved in 100 ml of MeOH A.R. and treated with saturated solution of hydrogen chloride in ethanol until the solution was pH = 1. The hydrochloride salt was precipitated out by addition of anhydrous ether. A white solid was collected and recrystallized from MeOH-ether (also decolorized with a small amount of activated charcoal) to produce 21.6 g (83% yield) of pure white crystals, m.p. 164-1660. Anal. Calc'd. for C27H28C1NO4.H2O: C, 67.00; H, 6.25; C1, 7.32; N, 2.89. Found: C, 67.10; H, 5.95; C1, 7.19; N, 2.65. Example 2 - Preparation of 7-(2-hydroxy-3-isopropylaminopropoxy) flavone hydrochloride 7- (2 ,3-Expoxypropoxy) flavone - Epichlorohydrin (231 g, 195.6 ml, 2.5 mol) was slowly added to a stirred yellow solution of 7-hydroxyflavone (119.0 g, 0.5 mol) in 50% aq EtOH (500 ml) containing NaOH (20.0 g, 0.5 mol) and left to stand at RT. It was left overnight and the white solid, 89.9 g, m.p. 252-40 (with some solid melting around 1200), was collected and washed with water. The original filtrate of the reaction mixture before water washing was stirred at RT overnight, yielding 73.6 g white solid after filtration and washing with water (at that time, tlc, 2% methanol in chloroform, indicated that only a small amount of the starting flavone was left). The first crop of the solid was suspended in chloroform (ca 250 ml), stirred for 30 min., and filtered. Evaporation of the chloroform solution afforded the desired epoxide as white crystals (21.12 g) m.p. 121-124 . Similarly, the second crop of the white solid gave 49.97 g of the epoxide m.p. 119-121 . The water washings from the first and second crops were combined and extracted with chloroform. The chloroform extracts were combined and evaporated to yield a white solid, which was washed with water to give 3.11 g, m.p. 114-1150. Water was added to the mother liquor from the second crop, and solid was collected. The solid was added to the filtrate, and it was extracted with chloroform twice. The extracts were evaporated to leave a reddish-gold oil which crystallized upon being seeded The sticky solid was collected and washed with a small amount of anhydrous ether to produce 12.53 g of white crystals, m.p. 120-1250. Total yield, 86.7 g, is 59%. It can also be prepared in 68% yield by refluxing under nitrogen 7-hydroxyflavone, epichlorohydrin or epibromohydrin (2 eq), anhydrous potassium carbonate (3 eq), and NaI (1.5 eq) in acetone for about 2 days. 7 - (2-Hydroxy-3-isopropylaminopropoxy) flavone hydrochloride - A suspension of 7-(2,3-epoxypropoxy)flavone 19.0 g, 0.065 mol) and isopropylamine 23.05 g, 33.2 ml, 0.39 mol) in methanol (AR, 250 ml) was stirred under nitrogen at RT. After 1.5 days' stirring, the reaction was not completed yet, as evidenced by tlc. It was then heated at 600 for 12 hrs. (to drive the reaction to completion). The solvent and excess of isopropylamine were removed under reduced pressure, thus leaving yellow crystals which, upon washing with anhydrous ether, gave a white solid (14.64 g), m.p. 140-1420.- The ether washing was evaporated and a yellow sticky solid obtained. It was washed with'a small amount of ether, but it remained sticky. Therefore, it was dissolved in a small amount of MeOH (AR) and treated with HCl-saturated EtOH until acidic. The solvents were removed, and the residue was dissolved in water (10 ml) and aq 10% HC1, extracted with chloroform (30 ml x 2). The aqueous layer was basified with conc. ammonia and extracted with chloroform (30 ml x 4). Chloroform extracts were washed with water and saturated Nail, and dried (MgSo4), thus affording 1.0 g of light brown crystals, m.p. 140-142". Combined yield, 15.64 g, was 68%. The free base (14.64 g) in MeOH (AR, 150 ml; a suspension) was treated wiht HCl-saturated ethanol (abs 2B) dropwise. It became a solution when the mixture was acidic, and then pale yellow crystals precipitated out. MeOH was added to make it to 400 ml and heated to bring it to a solution. It was concentrated to 250 ml, and anhydrous ether (800 ml) was added and cooled to give pale yellow prisms (15.92 g, 98.6% yield), m.p. 231-233 . The yellow color was gone upon drying at 800 under vaccum. Anal. Calc'd. for C2124ClNO4: C, 64.69; H, 6.20; C1, 9.09; N, 3.59; 0, 16.41. Found: C, 64.68; H, 6.20; C1, 9.02; N, 3.56; 0, 16.52. Example 3 - Preparation of 7-(2-hydroxy-3-isopropylamino propoxy)-3-phenylchromone hydrochloride: 7-(2,3-Epoxypropoxy)-3-phenylchromone - 7-Hydroxy3-phenylchromone and epichlorohydrin were reacted according to general procedure A, and the product obtained was white prisms, m.p. 151-152 (83% crude yield) from isopropyl alcohol. 7 - (2-Hydroxy-3- isopropylaminopropoxy) -3-phenyl- chromone hydrochloride - 7 - (2, 3-Epoxypropoxy) -3-phenyl- chromone and isopropylamine were reacted according to Method B to give the product as the hydrochloride in 62% yield, mp. 148-150 (EtAC-EtOH-Ether). Its corresponding free base, m.p. 128-130 . Anal. vCalc'd. for C21H24C1NO4: C, 64.69; C1, 9.09, H, 6.20; N, 3.59. Found: C, 64.63; H, 6.30; C1, 9.65; N, 3.56. Example 4 - Preparation of 7-(3-n-propylamino-2-hydroxypropoxy) flavone hydrochloride: Using Method B, the reaction of 7-(2,3-epoxypropoxy)-flavone and n-propylamine afforded the hydrochloride in 48% yield, m.p. 234-2350 (methonol). Its corresponding free base had a m.p. 140-145 . Anal. Calcsd. for C21H24ClNO4: C, 64.69; H, 6.20; C1, 9.09; N, 3.59; 0, 16.41. Found: C, 64.60; H, 6.37; C1, 8.44; N, 3.48; 0, 16.57 Example 5 - Preparation of 2'-chloro-7-(2-hydroxy-3 isopropylaminopropoxy) flavone 2'-Chloro-7-(2,3-epoxypropoxy)-flavone - The epoxypropoxy ether was prepared in quantitative yield (crude) from 2'-chloro-7-hydroxyflavone according to Method A. 2 1-Chloro-7- (2-hydroxy-3-isopropylaminopropoxy) flavone hydrochloride - The amine was prepared from the epoxide and isopropylamine according to Method B. The yield of recrystallized product (from toluene) was 56%, melting at 114-116 . Anal. Calc'd. for C21H22ClNO4: C, 65.03; H, 5.71: C1, 9.14; N, 3.61; 0, 16.41. Found: C, 65.22, H, 6.00; C1, 9.19; N, 3.56; 0, 15.55. Example 6 - Preparation of 6-(2-Hydroxy-3-isopropylaminopropoxy) flavone hydrochloride: 6-(2,3-Epoxypropoxy)flavone - This' epoxypropyl ether was obtained in 65% yield from 6-hydroxyflavone according to Method A. 6-(2-Hydroxy-3-isopropylaminopropoxy) flavone hydrochloride - The reaction of the above epoxide with isopropylamine according to Method B yielded (after recrystallization from n-butanol) 49% product, melting at 209-211". Anal. Calc'd. for C21H24ClNO4: C, 64.69; H, 6.20; Cl, 9.09; N, 3.59; 0, 16.41. Found: C, 64.59; H, 6.61; C1, 9.64; N, 3.40; 0, 16.02. Example 7 - Preparation of 4f-chloro-7-(2-hydroxy-3-iso- propylaminopropoxy) flavone hydrochloride 4'-Chloro-7-(2,3-epoxypropoxy)flavone - The epoxypropyl ether was prepared in quantiative yield (crude) from 4'-chloro-7-hydroxyflavone according to Method A. 42-Chloro-7-(2-hydroxy-3-isopropylaminopropoxy) flavone hydrochloride - The amine hydrochloride was prepared from the above epoxide and isoporpylamine using Method B. The yield of recrystallized product from dimethylformamide (DMF) was 63%, m.p. 258-259 . Anal. Calcsd. for C21H23C12NO4: C, 59.44; H, 5.46; C1, 16.71; N, 3.30; 0, 15.08. Found: C, 59.35; H, 560; C1, 16.96; N, 3.44; 0, 15.21. Example 8 - Preparation of 7-(3-¯-propylamino-2-hydroxy- propoxy) -2, 3-diphenylchromone hydrochloride The free base was prepared according to Method B from n-propylamine and 7-(2,3-epoxypropoxy)-2,3-diphenylchromone in-65% yield as white prisms, m.p. 140-142o. (iPrOH3. The hydrochloride salt was obtained in 95% yield as' white prisms, m.p. 134-136 (iPrOH). Example 9 - Preparation of 7-(3-cyclopropylamino-2hydroxypropoxy) flavone hydrochloride: Using Method B, the amine was prepared from cyclopropylamine and 7-(2,3-epoxypropoxy) flavone and purified by column chromatography, yield 26%. Treatment of the free base with HCl/EtOH solution gave the hydrochloride salt in 95% yield, m.p. 2140 (MeOH-ether). Anal. Calc'd. for C21H22C1NO4: C, 65.03; H, 5.71; C1, 9.14; N, 3.61; 0, 16.49. Found: C, 64.82; H, 5.81; C1, 9.27; N, 3.65; 0, 16.78. Example 10 - Preparation of 7-(3-Methylamino-2-hydroxypropoxy) flavone hydrochloride: The free amine was prepared according to Method B from 7-(2,3-epoxypropoxy) flavone and 40% aqueous methylamine in 30% yield after column purification, m.p. 133-40 (MeOH-CH2Cl2). The hydrochloride salt, m.p. 251-520 (MeOH); yield 68% (purified). Anal. Calc'd. for ClgH20ClNO4.H2O: C, 61.54; H, 5.71; C1, 9.56; N, 3.78; 0, 19.42. Found: C, 61.47; H, 5.82; C1, 9.88; N, 3.63; 0, 19,92. Example 11 - Preparation of 7-(3-Ethylamino-2-hydroxypropoxy) flavone hydrochloride: According to Method B, the title compound was synthesized from the reaction of 7-(2,3-epoxypropoxy) flavone and 70% aqueous ethyl amine followed by treatment with HCl/EtOH, as white prisms, m.p. 244-460 (MeOH) in 36% yield. The free base was obtained after column purification as white crystals, m.p. 164-65 (MeOH/ether), yield 39%. Anal. Calc'd. for C2oR2ClNO4: C, 63.91; H, 5.89; C1, 9.43; N, 3.72; 0, 17.02. Found: C, 64.07; H, 6.03; C1, 9.65; N, 3.76; 0, 17.14. Example 12 - Preparation of 7-(3-n-butylamino-2-hydroxypropoxy) flavone hydrochloride Using Method B, the reaction of 7-(2,3-epoxypropoxy) flavone and n-butylamine afforded the hydrochloride in 35% yield (purified) as white crystals, m.p. 224-250 (MeOH). The free base was purified by high pressure liquid chromatogrpahy as an off-white powder, m.p. 143-44 (53NO yield). Anal. Calc'd. for C22H26C1NO4: C, 65.42; H, 6.48; C1, 8.77; N, 3.46; 0, 15.84. Found: C, 65.01; H, 6.83; C1, 9.08; N, 3.33; 0, 15.41. Example 13 - Preparation of 7-(3-cyclohexylamino-2hydroxypropoxy) flavone hydrochloride: The title compound was prepared according to Method B in 63% yield as a white powder, m.p. 242-430 (MeOH). Its free base was purified by high,pressure liquid chromatogrphy and obtained as white powder, m.p. 174760 (63% yield). Anal. Calc'd. for C24H28C1NO4.4H2O: C, 65.67; H, 6.66; N, 3,19; 0, 16.40. Found: C, 65.34; H, 6.87; N, 3.16; 0, 16.86. Example 14 - Preparation of 7-(3-cyclopropylamino-2hydroxypropoxy)-2,3-diphenylchromone: The amine was prepared according to Method B from cyclopropylamine and 7-(2,3-epoxypropoxy)-2,3-diphenylchromone and purified by high pressure liquid chromatography to give white crystals, m.p. 135-137 (MeOH-ether), in 25% yield. Example 15 - Preparation o f 7 - of 7-(3-amino-2-hydroxypropoxy) flavone hydrochloride: 7-(2,3-Epoxypropoxy)flavone (13.3 g, 0.045 mol) in a pressure bottle was treated with a methanolic solution (90 ml) containing 1.87 g of ammonia (0.11 mol). The bottle was sealed and the suspension was heated at 500 for 12 hrs. A tlc (15% MeOH/CHC13) indicated that the starting material was gone, and the reaction mixture was cooled and filtered. The solid (5.32 g) thus obtained was shown by tlc to be a byproduct, m,p. 209-12 . The filtrate, upon evaporation, afforded 7.0 g of a yellow solid, m.p. 121-270, which was purified by high pressure liquid chromatography to give 1.85 g of yellow powder, m.p. 154-56 . (13% yield). The hydrochloride salt was prepared and recrystallized from MeOH, m.p. 191-20. Example 16 - Preparation of 7-(3-propylamino-2-hydroxypropoxy)-3-benzylflavone hydrochloride: 3-Benzyl-7- (2 ,3-epoxypropoxy) flavone - Using Method A, 3-benzyl-7-hydroxyflavone was converted to its corresponding epoxide, m.p. 133-1380. 7- (2-hydroxy-3-propylaminopropoxy) -3-benzylflavone hydrochloride - The amine salt was prepared from 3-benzyl-7-(2,3-epoxypropoxy)flavone as described in Method B as white prisms, m.p. 196-80 (iPrOH); yield, ;50% (from the epoxide). Anal. Calc'd. for C23H30C1NO4: C, 70.07; H, 6.30; Cl, 7.39; N, 2.92. Found: C, 69.78; H, 6.32; C1, 7.49; N, 2.74. Example 17 - Preparation of 7-(2-hydroxy-3-isopropylaminopropoxy)chrysin hydrochloride: 5-Hydroxy-7-(2,3-epoxypropoxy)flavone - Using Method A, the epoxide was prepared as a light yellow solid from chrysin hydrochloride in 26% yield (purified); m.p. 169-170 , Anal. Calc'd. for C18H13O5: C, 69.89; H, 4.235 0, 25.86. Found: C, 69,47; H, 4.72; 0, 26.04. 7- (2-hydroxy-3-isopropylaminopropoxy) chrysin hydrochloride - The hydrochloride was prepared as usual from the above epoxide and isopropylamine as a light yellow powder; m.p. 236-370. (dec. iPrOH); 45% yield. Anal. Calc'd. for C21H24ClNO: C, 62.14; H, 5.96; C1, 8.73; N, 3.45. Found: C, 61.90; H, 5.84; C1, 8.72; N, 3.33. Example 18 - Preparation of 7-(2-hydroxy-3-isopropyl aminopropoxy)-2-methylisoflavone hydrochloride: 7- (2 ,3-epoxypropoxy)-2-methylisoflavone - Using Method A, the epoxide was synthesized from 7-hydro-2methylisoflavone as white prisms. 7-(2-hydroxy-3-isopropylaminopropoxy)-2-methylisoflavone hydrochloride - The title compound was prepared as described in Method B from the above epoxide and isopropylamine as white prisms; m.p. 183-50 (iPrOH), in 40% yield. Anal. Calc'd. for C22H26ClNO4: C, 65.42; H, 6.49; N, 3.47; Cl, 8.78. Found: C, 65.25; H, 6.74; N, 3.39; C1, 8.67. Example 19 - Preparation of 7-(3-N-methyl-N-isopropylamino-2-hydroxypropoxy)-2-methylisoflavone methiodide: 7- (2-hydroxy-3-isopropylaminopropoxy)-2-methyliso- flavone (8.1 g, 0.021 moles) was added to a small amount of dry ether. The etheral solution under nitrogen was allowed to react with methyl iodide (9.0 ml) and stirred overnight. The solid formed was collected and washed with anhydrous ether. It was recrystallized twice from ethanol (abs.) to give 6.8 g (62% yield) of light yellow powder; m.p. 211-2130C. Anal. Calc'd. for C24H30NO41: C, 55.07; H, 5.77, 1, 24.24; N, 2.67. Found; C, 54.89; H, 5.80; I, 24.06; N, 2.49 Example 20 - Preparation of 7-(2-hydroxy-3-isopropyl aminopropoxy) -2-trifluoromethylisoflavone hydrochloride: 7-(2ffi3¯epoxypropoxy)-2-trifluoromethylisoflavone The epoxide was prepared from 7-hydroxy-2-trifluoromethylisoflavone following Method A: m.p. 146-148 (CH2Cl2); yield, 61%. 7- (2-hydroxy-3-isopropylaminopropoxy) -2-trifluoro- methylisoflavone hydrochloride - Reaction of the corresponding epoxide with isopropylamine, as shown in Method B, afforded the title compound as white prisms, m.p. 220-2220C. Anal. Calc'd. for C22H23ClFNO4: C, 57.71; H, 5.06; C1, 7.74; F, 12.44; N, 3.05. Found: C, 57.88; H, 5.06; C1, 7.52; F, 12.51; N, 2.97. Example 21 - Preparation of 7-(2-hydroxy-3-isopropylaminopropoxy) -2-isopropylisoflavone maleate: 7-(2,3-epoxypropoxy)-2-isopropylisoflavone - The epoxide was prepared from 7-hydroxy-2-isopropylisoflavone by Method A; m.p. 139-1400C (CH2C12); yield, 27%. 7-(2-hydroxy-3-isopropylaminopropoxy) -2-isopropyl- isoflavone maleate - The corresponding epoxide was reacted with isopropylamine followed by maleic acid to give the matleate as white prisms in 94% yield, m.p. 187-1880. Anal. Calc'd. for C28H33NO8: C, 65.74; H, 6.50; N, 2.73. Found: C, 65.77; H, 6.27;, N, 2.66. Example 22 - Preparation of 7-(3-allylamino-2-hydroxy propoxy) -2 ,3-diphenylchromone: Using Method B, the reaction of 7-(2,3-epoxypropoxy) -2,3-diphenychromone and allylamine afforded the desired amine as white needles, m.p. 119- (iPrOH); yield, 82%. Its hydrochloride salt had m.p. 140-1420 (iPrOH/phCH3); containing water). Anal. Calc'd. for C27H25NO4 (the free base): C, 75.85; H, 5.89; N, 3.27; 0, 14.97. Found: C, 75.87; H, 5.95; N, 3.14; 0, 15.04 Example 23 - Preparation of 7-(3-cyclopentylamin-2-hy- droxypropoxy)-2,3-diphenylchromone hydrochloride: Using Method B, the reaction of 7-(2,3-epoxypropoxy) -2,3-diphenylchromone and cyclopentylamine gave the title amine as white crystals; m.p. 162.5-163 (iPrOH); yield 87%. Its hydrochloride salt was prepared as usual and melted at i551560 (iPrOH). Anal. Calc'd. for C29H30ClN04.14H2O: C, 67.11; H, 6.41; C1, 6.83; N, 2.70; 0, 16.95. Found: C, 67.04; H, 5.90; C1, 6.70; N, 2.66; 0, 17.04. Example 24 - Preparation of 7-(3-sec-butylamine-2-hydroxypropoxy)-2,3-diphenylchromone The title compound was synthesized according to Method B, in 98% yield, as white crystals, m.p. 139-139.5 . Anal. Calc'd. for C28H29NO4: C, 75.82; H, 6.59; N, 3.15. Found: C, 75.50; H, 6.81; N, 3.10. Example 25 - Preparation of 7-(3-N-methyl-N-propylamino2-hydroxypropoxy)-2,3-diphenylchromone maleate Using Method B, the free base of the title compound was prepared from the corresponding epoxide and N-methylpropylamine. The obtained amine was dissolved in isopropyl alcohol and reacted with a solution of maleic acid in isoporpyl alcohol. The white crystals formed were collected; m.p. 135-1370. Anal. Calc'd. for C32H33NO8: C, 68.68; H, 5.94; N, 2.50. Found; C, 68.88; H, 5.98; N, 2.26. Example 26 - Preparation of 3-Methyl-7-(3-isopropylamino2-hydroxypropoxy) flavone hydrochloride 3-Methyl-7-(2,3-epoxypropoxy)flavone - Using Method A, 3-methyl-7-hydroxyflavone was converted to its corresponding 7-epoxypropyl ether, m.p. 231-2350 (crude); yield 86%. 3-Methyl-7- (2-hydroxy-3-isopropylaminopropoxy) flavone hydrochloride - The amine salt was prepared as described in Method B to give a white solid, m.p. 141-144 . Anal. Calc'd. for C22H26ClNO4: C, 65.42; H, 6.48; C1, 8.77; N, 3.46; 0, 15.84. Found: C, 65.62; H, 6.61; C1, 8.51; N, 3.33; 0, 15.30. Antihypertensive activity was determined in the spontaneously hypertensive rat. Animals were dosed with compound or control vehicle following a control blood presssure (systolic) and heart rate determination made by means of an inflatable tail cuff. Measurements were made contained at 5 hours post dosing. Five animals per test group were employed. A 10% decrease in systolic blood pressure which was statistically different from both the control animals and the post dosage measurement is classified as minimal activity. The following table gives minimal dosage required for minimal activity at 5 hours for the compounds of formula (1) and for a compound of the P. Da Re article, 3-methyl-7- (3-isopropylamino-2-hydroxypropoxy)flavone hydrochloride prepared using Method A and Method B, and shown in Example 26. The compounds in which R2 and R3 are both phenyl are surprisingly found to have distinctly superior advantages over those compounds in which R3 is hydrogen, particularly for longer durations of activity. The compounds in which R1 is cyclopropyl and n-propyl are also found to exhibit surprisingly superior activity. Example 27 Pharmacology Data Activity Example No. R1 R2 R3 R4 mg./kg. 1 i-propyl phenyl phenyl hydrogen 8 2 " " hydrogen " 17 3 " hydrogen phenyl " 75 4 n-propyl phenyl hydrogen " 12 5 i-propyl 2'C1 phenyl " " 35 6 " phenyl " " 75 7 " 4'C1 phenyl " " 35 8 n-propyl phenyl phenyl " 8 9 cyclopropyl " hydrogen " 8 10 methyl " 1I " 35 11 ethyl " " " 35 12 n-butyl I? " " 17 13 cyclohexyl ti " " 17 14 cyclopropyl " phenyl " 8 15 hydrogen " hydrogen " 75 16 n-propyl " benzyl " 75 17 i-propyl " hydrogen hydroxyl 75 18 ,t methyl phenyl hydrogen 35 N1N-dimethyl 19 isopropylamino " phenyl hydrogen 75 20 i-propyl CF3 ri 35 21 " i-propyl " " 35 22 allyl 'phenyl li " 75 23 cyclopentyl :1 " " 75 24 sec-butyl " II " 75 25 N-methyl-N propyl " tI 75 26 i-propyl " methyl " 75 These compounds do not exhibit beta-blocking activity when determined as antagonism of isoproternol-induced beta stimulation of isolated rat heart or inhibition of isoproternol-induced effects on the cardiovascular system of the anesthetized rat.

Claims: WHAT IS CLAIMED IS: 1* A pharmaceutical composition in unit dosage form comprising (a) a hypertension-reducing amount of a chromone of the formula EMI21.1 or a pharmaceutically acceptable salt thereof wherein the R1-NHCH2-CH(OH)-CH2O- group is in the 6 or 7 position of the chromone nucleus; R1 is H, primary or secondary alkyl of 1 to 4 carbons, or cycloalkyl of 3 to 6 carbons; R2 is H, lower alkyl, CF3, phenyl, o-chlorophenyl or p-chlorophenyl; R3 is H or phenyl; and R4 is hydrogen or hydroxyl; provided, however, that at least one of R2 or R3 is phenyl or substituted phenyl; and (b) a pharmaceutically acceptable carrier. 2. The composition of claim 1 wherein the R1NHCH2-CH(OH)CH2- group is in the 7 position of the chromone nucleus; R1 is i-propyl, n-propyl or cyclopropyl; R2 and R3 are phenyl and R4 is hydrogen. 3. The composition of claim 1 wherein the R,1NHCH2-CH(OH)-CH2O- group is in the 7 position of the chromone nucleus; R1 is i-propyl, n-propyl, or cyclopropyl; R2 and R3 are phenyl and R4 is hydrogen. 4. The composition of claim 1 wherein the chromone is 7- (3-n.-propylamino-2-hydroxypropoxy)-2 ,3-diphenylchromone hydrochloride. 5. A compound having the formula EMI22.1 or a pharmaceutically acceptable salt thereof wherein R1 is isopropyl, n-propyl or N1N-dimethyl-N-isopropyl and R2 is hydrogen, methyl, CF3 or isopropyl. 6. The compound of claim 5, 7-(2-hydroxy-3-isopropylaminopropoxy)-3-phenylchromone hydrochloride. 7. The compound of claim 5, 7-(2-hydroxy-3-isopropyl aminopropoxy) -2-methylisoflavone hydrochloride'. ,8. The compound of claim 5, 7-(3-N1N-dimethyl-N-iso propylamino-2-hydroxypropoxy) -2-methylisoflavone methiodide. 9. The compound of claim ,5, 7-(2-hydroxy-3-isopro- pylaminopropoxy)-2-trifluoromethylisoflavone hydrochloride. 10. The compound of claim 5, 7-(2-hydroxy-3-isopropylaminopropoxy)-2-isopropylisoflavone maleate. CLAIM FOR AUSTRIA: Process for the production of a chromone having the formula EMI23.1 or a pharmaceutically acceptable salt thereof wherein the R1NHCH2-CH(OH)-CH2O- group is in the 6 or 7 position of the chromone nucleus; R1 is H, primary or secondary alkyl of 1 to 4 carbons; or cycloalkyl of, 3 to 6 carbons; R2 is H, lower alkyl, CF3, phenyl, o-chlorophenyl or p-chlorophenyl, R3 is H or phenyl or benzyl and R4 is hydrogen or hydroxyl; provided, however, that at least one of R2 or R3is phenyl or substituted phenyl, characterized by reacting epichlorohydrin or epibromohydrin with a compound of the formula EMI23.2 wherein R3 is the same as defined above and OR5 represents a hydroxyl group substituted on the 7 position, in the presence of a solvent and a base to give a product of the above formula where OR5 is EMI23.3 and reacting this epoxide with an amine, R1NH2 or R1R6NH 6, where R1 is as above defined and R6 is hydrogen or benzyl, in an alcoholic solvent at elevated temperature, and,,in case R6 being benzyl, catalytically hydrogenating this compound to give the secondary amine and optionally converting the obtained compound into a pharmaceutically acceptable salt thereof'.

Inventors: WU, EDWIN SHEN-CHOU
Applicants: PENNWALT CORPORATION
Application year: 1982
Main classification: C07D311
IPC codes: A61P9, A61K31, C07D311