LOW-PRESSURE FIXATION OF VALVULAR TISSUE INTENDED FOR IMPLANTATION

Abstract: A method of obtaining fixation of an animal aortic valve intended for implantation, including subjecting the aortic valve (13) to a tanning fluid at a differential pressure across the valve leaflets (21 and 25) in a direction to urge the valve leaflets closed and with the tanning fluid tending to distort the aortic valve. The differential pressure across the valve leaflets is greater than zero and less than about four mm Hg. An insert (17) restrains the valve (13) against substantial distortion during the time the valve is subjected to the tanning fluid.

Description: LO -PRESSURE FIXATION OF VALVULAR TISSUE INTENDED FOR IMPLANTATION BACKGROUND OF TKΞ INVENTION Animal heart valves, such as porcine heart valves, have been used for implantation in humans. To 5 prepare the animal heart valve for implantation, it is common practice to tan the valve. Tanning renders the animal tissue relatively inert with respect to the liv¬ ing host environment and brings about fixation of the tissue, i.e., stabilization of the tissue so that it 10 has a fixed configuration. As disclosed in Hancock et al U.S. Patent Nos . 3,966,401 and 4,050,893 and Angell et al U.S. Patent No. 3,983,581, it is known to tan animal heart valves by ___ • utilizing a tanning fluid under pressures which may 15 range from 20 mm Hg to 120 mm Hg. These methods pro¬ vide a substantial internal pressure on the heart valve. According to Hancock et al Patent No. 4,050,893, the use of pressures in the physiologic range, i.e., 80-120 mm Hg causes the tissue to assume substantially its natural 0 configuration during the tanning process and in fixing the valve in its natural contour so that it will retain its shape following the tanning process. Recent reports by Broom, Neil D. , An In Vitro Study of Mechanical Fatigue in Glutaraldeh de-Treated 5 Porcine Aortic Valve Tissue, Bio aterials, Volume 1, O PI January 3 , 1980 , and Broom, Neil D. and Thomson , Fergus J. , Influence of Fixation Conditions on the Performance of Glutaraldeh — de-Treated Porcine Aortic Valves : Towards A More Scientific Basis, Thorax, Volume 34, 1979, describe problems that occur in the structure of the valve leaflets during tanning under pressures at the physiological levels. Briefly, the valve tissue of a porcine aortic valve in- eludes collagen fibers arranged in a waveform or crimped condition. When internal pressures are applied to the valve curing the tanning process, the collagen waveform tends to straighten out, and the degree to which the wave¬ form returns to its original condition following removal of the internal pressure is a function of the magnitude of the internal pressure applied during the tanning pro¬ cess. If the collagen does not return to its original waveform condition, the stiffness of the valve leaflet is adversely affected. This has an adverse influence on the manner in which the valve leaflets open, and it pro¬ vides regions of local strain or kinks in the valve leaflets during opening. It is believed that loss of the waveform geometry also reduces the fatigue durability of the valve leaflets. O PI According to these publications , obtaining fixa¬ tion at the higher internal pressure levels results in considerable permanent loss of the collagen waveform so that, upon removal of the relatively high internal pres¬ sure , much of the collagen does not return to its origi¬ nal waveform condition. It is- further reported by the same authors that a pressure of 100 mm Hg eliminates the waveform geometry entirely and that pressures even as low as 4 mm Hg result in significant reductions in the wave¬ form after removal of the internal pressure . To avoid these undesirable results , it is sug¬ gested in the above-noted publications that the tanning process should be carried out at pressures in the range of slightly above zero to about 4 mm Hg. However, an in¬ herent effect of subjecting the valve to the tanning flu¬ id is that the valve tends to shrink and distort. The relatively low internal pressure of just above zero to about 4 mm Hg is insufficient to retain the animal heart valve against shrinkage and distortion during the tanning process . Accordingly, the valve may become fixed in a configuration which is unsuited for use in an implant. One prior art method for tanning at essentially zero pressure differential across the valve leaflet is to O PI - ~ -. _- pack the valve leaflet with a batting, such as a Dacron or cotton batting, to assure that the leaflets contact 5 each other to provide a competent valve. However, great care must be taken in packing the delicate valve leaf¬ lets, and . even with the exercise of considerable care and diligence in packing, the mechanical stresses exerted on the valve leaflets cannot be measured and are subject to 10 operator variability. In addition, abrasion between the valve leaflets and the batting results in changes in sur¬ face morphology, and a substantial amount of time is re¬ quired to carefully pack the valve leaflets with, the bat- _-___ ting. 15 SUMMARY OF THE INVENTION This invention accomplishes " fixation of an ani¬ mal heart valve by subjecting the valve to a tanning flu- 20 id at relatively low pressures to avoid permanent loss of the collagen waveform. During the tanning process, the valve is mechanically restrained to substantially avoid shrinkage and distortion, and packing the valve leaflets is eliminated. _ __ With this invention, an animal aortic heart valve is subjected to a tanning fluid at a differential pressure across the valve leaflets in a direction to urge the valve leaflets closed. The differential pressure should be sufficient to hold the valve leaflets closed, and, therefore, should be greater than zero. On the other hand, the differential pressure should not be so great as to provide a significant percentage of irrever¬ sible alterations in the waveform geometry of the colla¬ gen of the valve ,leaflets. For a porcine aortic valve and based upon experimental data presently available, this establishes an upper differential pressure limit of about 4 mm Hg. although particular circumstances and/or additional experimental data may prove that 4 mm Ξg is not an absolute upper differential pressure limit. Because the internal pressure on the valve is insufficient to restrain the valve against distortion dur- ing the tanning process, this invention provides for other forms of restraints. Preferably, restraint is accomplish¬ ed mechanically by inserting an insert into the aortic valve so that the insert is present for restraining pur¬ poses during the tanning process. The insert has numer- ous features which, when appropriately employed, provide -BUREAU OMPI a particularly advantageous restraint against the distor¬ tion which inherently tends to occur as a result of the tanning process. To illustrate, an aortic valve may be consider¬ ed as including a tubular aorta segment and three delicate valve leaflets, each of which is affixed to the -aorta seg¬ ment at a juncture. The insert preferably has a leading end and is inserted into the aorta segment to a location at which the leading end is closely adjacent the junc¬ tures. When the valve is subjected to a tanning fluid, it tends to shrink and this tends to draw the valve leaf¬ lets inwardly. By inserting the insert to this location, the insert appropriately engages the valve to substantial¬ ly prevent it from shrinking and distorting. The insert is preferably relatively rigid and it must have sufficient rigidity to enable it to retain the valve against distor¬ tion. It is desirable to avoid contacting the deli¬ cate valve leaflets with the insert, and accordingly, the insert preferably engages the valve on the side of the junctures remote from the valve leaflets. Also, the in¬ sert may advantageously include three axially extending projections separated circumferentially by recesses. In the installed condition, the three projections engage and restrain the valve adjacent the junctures, respec- tively, and the recesses confront the commissures to minimize shrinkage and distortion of the aortic tissue at the valve annulus and in the area of the sinuses of Valsalva. The aortic valve is not symmetrical. More spe- cifically, the interventricular wall extends to the base of the right coronary valve leaflet making a kind of sup¬ port for this valve leaflet. The muscular part of this leaflet can be weakened by trimming, but in any event, the right coronary valve leaflet provides a slight asym- etry. To accommodate this, one of the projections has an outer tissue supporting surface adjacent its free end which lies more radially outwardly than the correspond¬ ing outer tissue supporting surfaces of the other two pro¬ jections. This projection, i.e., the one with an outer surface which lies more radially outwardly than the others, is located in the sinus of Valsalva of the right coronary valve leaflet for contact with the wall of the aorta seg¬ ment. This asymmetry of the insert and its orientation are very important in minimizing shrinkage and distortion near the right coronary valve leaflet. The asymmetry of O PI r W1P0 - 3- the insert can be brought about in different ways, such as by different angles of radial outward inclination on the projections, providing segments near the free ends of each of the projections which project radially outwardly differ¬ ent distances, etc. To avoid damaging the valve, the edges of the in¬ sert which contact the tissue are' rounded in elevation and in axial cross section. The insert is preferably tubular to enable a tanning fluid to be supplied to the interior of the valve through the insert. The insert can be con¬ structed of any biocompatible material which is inert to the tanning fluid being utilized and is preferably trans- parent to facilitat .visual placement of the insert in the valve. The invention, together with additional features and advantages thereof, may best be understood by refer¬ ence to the following description taken in connection with the accompanying illustrative drawings. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a somewhat schematic sectional view illustrating an apparatus for tanning animal aortic valves in accordance with the teachings of this invention. 'gU EAϋ O PI _ Fig. 2 is a sectional view on line 2-2 of Fig. 4 of one form of insert constructed in accordance with the teachings of this invention inserted into a porcine aor¬ tic valve and clamped in position. Fig. 3 is- a perspective view of the insert. Fig. 4 is a top plan view of the aortic valve of Fig. 2. Fig. 5 is a simplified view of the insert and valve of Fig. 2 laid flat to show the zone of contact be¬ tween the two. Fig. 6 is a plot of differential pressure across the valve leaflets and collagen strain. DESCRIPTION OF THE PREFERRED EMBODIMENT Fig. 1 shows an apparatus 11 for obtaining low- pressure fixation of a porcine aortic valve 13 utilizing a tanning solution 15 and an insert 17. With reference to Figs. 2 and 4, the aortic valve 13 has been excised from the heart and it includes a tubular aorta segment which, in the form illustrated, is a portion of the as¬ cending aorta 19 and three valve leaflets 21, 23 and 25. The bulk of the myocardial tissue has been trimmed from the valve 13 to minimize distortion during fixation, and the coronary arteries (not shown) are tied off. Adjacent edges of the valve leaflets 21, 23 and 25 meet to form commissures 27 at the junctions between adjacent valve leaflets. Each of the valve leaflets 21, 23 and 25 may be considered as joined to the ascending aorta 19 along a juncture 29. The wall of the ascending aorta 19 adjacent the junctures 29 forms sinuses 31 of Valsalva. The valve leaflet 21 is the right coronary leaflet. The insert 17 in the embodiment illustrated is integrally molded from a suitable biocompatible material which is compatible with the tanning solution 15, such as a suitable polyester or acrylic material. The insert 17 -r is preferably sufficiently transparent so that its posi¬ tion within the aortic valve 13 can be accurately visu- ally observed. The insert 17 includes a tubular peripheral wall 37 of a generally cylindrical configuration which is open at both ends and which defines a generally axial cylindri¬ cal passage 39 extending through the insert. The periph¬ eral wall has three generally axially extending projec¬ tions 41, 43 and 45, with each adjacent pair of projec- tions being circumferentially spaced by a recess 47. The -ι; projection 41 terminates outwardly at its free end in a segment or rib 49, and the projections 43 and 45 similar- ly terminate at their free ends in ribs 51- As best seen in Fig. 2 , the ribs 49 and 51 project radially outwardly to provide outer tissue supporting surfaces 53 and 55, respectively. The recesses 47 prevent contact with, and damage to, the valve leaflets 21, 23 and 25 at the com- rnissures 27. To avoid damaging the tissue which they con¬ tact in the valve 13, the ribs 49 and 51 and the free ends of the projections 41, 43 and 45, in the embodiment illus¬ trated, are curved in axial cross section as shown in Fig. 2 and are also curved as viewed in side elevation as shown in Fig. 3. The recesses 47 are " similarly formed " by smooth¬ ly curved edges on the projections 41, 43 and 45. The leading end portion, i.e., the upper end portion as view¬ ed in Figs. 2 and 3, is asymmetrical about an axis 57 which is the central axis of the passage 39 and of the insert 17. Specifically, the radial spacing between the outer tissue supporting surface 53 and the axis 57 is greater than the radial spacing between the axis 57 and the outer tissue supporting surfaces 55. The radial spacing between both of the outer tissue supporting "BUREA U Oi PI surf ces 55 and the axis 57 is the same. In the embodi¬ ment illustrated, the projections extend slightly radial- ly outwardly as they extend toward their free ends. To carry out the tanning process and obtain fixation of the valve 13, the leading end of the insert 17 is inserted into the open end of the ascending aorta 19 to the location shown in Fig. 2. The valve 13 is then suitably affixed to the insert 17 as by sutures (not shown) or a strap 59 which encircles the aorta 19 adjacent its open end and clamps it to the insert. The strap 59 holds the valve 13 against axial movement rela¬ tive to the insert 17 and should not be placed on any part of the va^ve which will be used in the implant made from the valve 13. When in position within the valve 13, the pro¬ jections 41, 43 and 45 engage, or lie closely adjacent, the junctures 29, respectively, and the recesses 47 confront, and lie radially inwardly of, the commissures 27, respectively, as shown in Fig. 2. The insert 17 does not contact the valve leaflets 21, 23 and 25. The projection 41 is provided for supporting the valve 13 in the region of the right coronary so that the outer tissue supporting surface 53 of the rib 49 engages the -ΪTUREAIΓ O PI aortic wall of the aorta 19 adjacent the right coronary valve leaflet 21. The ribs 49 and 51 engage and support the aortic wall of the aorta in the sinuses of Valsalva 31. Except for the sinuses of Valsalva, the outside dia¬ meter of the insert 17 is approximately equal to the in¬ side diameter of the adjacent regions of the ascending aorta 19. The radial spacing between the axis 57 and the outer tissue supporting surfaces 53 and 55 represents the desired position beyond which the adjacent junctures 29 should not be allowed to distort or shrink. Fig. 5 shows how the projections 41, 43 and 45 thoroughly support the valve 13 without contacting the valve leaflets_,21, 23 and 25. As viewed in Fig. 5, the junctures 29 are generally semicircular, and the ribs 49 and 51 engage the aorta 19 just below the junctures. The recesses 47 and the curvature (as seen in Fig. 5) of the projections prevent contact of the insert with the valve leaflets. The apparatus 11 may be used to tan one or more of the valves 13 which have an insert 17 within them as shown in Fig. 2. The apparatus 11 includes a tank ' 61, a header 63, a reservoir 65 coupled to the head¬ er 63, a pump 67, an intake conduit 69 leading from the -BURE U OMPI tanning solution 15 within the tank to the intake of the pump, and a discharge conduit 71 leading from the dis- charge of the pump to the header 63. The header 63 is fixed within the tank 61 and has a passage 73 coupled to the conduit 71 and to the lower end of the reservoir 65. The tanning solution 15 fills the tank 61 to a pre¬ determined height, and the reservoir 65 has an open top 71 which lies a prescribed distance above the elevation of the tanning solution 15 in the tank 61. The difference in elevation between the levels of the tanning solution 15 within the tank 61 and the reservoir 65 represents the differential pressure across the valve leaflets 21, 23 and 25 at whic the tanning process will be carried out. This is a static head and flow is required only to make up for leakage, and a slight initial flow is re¬ quired to close the valve leaflets 21, 23 and 25. In the embodiment illustrated, the head represented by the dif- ference in these two elevations is 2 -___. Hg. The passage 73 in the header 63 also communi¬ cates with a riser 77 having a stopper 79 mounted on it. The upper end of the stopper 79 is received within the lower end of the insert 17 so that the stopper and in- sert cooperate to mount the valve 13 vertically within the tank 61. Additional valves 13 may be similarly mounted on the header 63, if desired. fURElT- OMPI The tanning solution 15 may be of any composi¬ tion suitable for tanning the porcine aortic valve 13. For example, the tanning solution 15 may be a suitable solution of glutaraldehyde. • With the components in the position of Fig. 1, the pump 67 can be operated to pump tanning solution 15 from the tank 61 through the conduits 69 and 71, the head- er 63 and over the top 75 of the reservoir 65 as may be required to maintain the desired static head. The flow if any, is exceedingly small to avoid subjecting the leaflets 21, 23 and 25 to any velocity head. Thus, the interior of the valve 13, including the interior of the valve leaflets 21, 23 and 25, is subject to a static pressure increasing to the height of the top 75. Simul¬ taneously, the outer surfaces of the valve 13 are sub¬ jected to the tanning solution 15 at a static pressure corresponding to the elevation of the tanning solution in the tank 61. Thus, the differential pressure to which the aortic valve 13, and in particular, the delicate valve leaflets 21, 23 and 25 is subjected corresponds to the difference in elevation between the top 75 of the reservoir 65 and the elevation of the tanning solu- tion 15 within the tank 61. This assures that the internal pressure within the valve 13 will exceed the external pressure so that the valve leaflets 21, 23 and 25 will be urged toward the closed position. Also, be¬ cause the liquid level in the reservoir 65 cannot rise above the top 75, the maximum internal pressure is also regulated. By utilizing a known volume of the tanning solution 15 in- containers of known volume, the differ- ential pressure across the valve leaflets 21, 23 and 25 can be maintained at the desired nominal value. The action of the tanning solution 15 on the valve 13 tends to shrink and distort the valve. How¬ ever, the engagement of the valve 13 in the region of the sinuses of Valsalva 31 against the relatively rigid projections 41, 43 and 45 prevents significant distor¬ tion of these critical portions of the valve during the tanning process. The tissue adjacent the right coronary valve leaflet 21 contains additional muscle as shown in Fig. 2. The specially configured projection 41 engages the aortic wall adjacent the right coronary leaflet 21 to prevent significant distortion and shrinkage in this region. Also, the lower regions of the valve 13 engage the outer periphery of the insert 17 at and below the - projections 41, 43 and 45 to also hold these regions against shrinkage and distortion. The valve 13 is sub¬ jected to the action of the tanning solution 15 for a sufficient time to obtain fixation of the valve, and this may take, for example, about eight hours. Fig. 6 shows the relationship between valve leaflet deformation as a function of differential pres¬ sure across the valve leaflet during the tanning process As the differential pressure rises from zero to about 4 mm Hg, the valve leaflets deform linearly along a seg¬ ment 101 of the curve. During this time, deformation is relatively substantial due to extension of the wave¬ form of the collagen or uncrimping of the collagen. Generally, the deformation represented by the segment 101 is not harmful because, when the differential pres¬ sure is removed, the collagen returns to its original waveform or crimped condition. However, above about 4 mm Hg of differential pressure, the rate of deformation i s substantially reduced, and this is graphically indi¬ cated by a segment 103 of the curve which has a much steeper slope; thus, indicating a much slower rate of change of deformation with respect to differential pres¬ sure. The deformation of the valve leaflets represented by the segment 103 of the curve results in permanently removing, to a degree that increases with pressure, the collagen waveform rather than elastically stretching or uncrimping of the collagen. This destruction of the collagen waveform produces the harmful affects describ¬ ed above. Available experimental data indicates that the separation of the segments 101 and 103 is at about 4 mm Hg differential pressure, although some modest variation in this could reasonably be expected in that tissue is not homogeneous, and thus all heart valves upon which the process is performed would not be expect¬ ed to have identical structural properties. Also, as new experimental data becomes available, refinements of the upper limit on the differential pressure at which collagen straining becomes unacceptable may become known. Of course, some straining of the collagen may occur at under 4 mmHg but generally it is believed that collagen strain at differential pressures under 4 mm Hg is not particularly harmful. Although an exemplary embodiment of the inven¬ tion has been shown and described, many changes, modi¬ fications and substitutions may be made by one having ordinary skill in the art without necessarily departing from the spirit and scope of this invention.

Claims: CLAIMS 1. A method of tanning an animal aortic valve wherein the aortic valve includes an aorta seg¬ ment and valve leaflets affixed to the aorta segment said method comprising: • subjecting the valve to a tanning fluid at a differential pressure across the valve leaflets in the range of greater than zero and no more than about four millimeters of mercury with the differential pressure being in a direction to urge the valve leaf- lets closed to tan the valve and to tend to distort the valve; and^ mechanically restraining the valve against substantial distortion during said step of subjecting- 2. A method as defined in claim 1 wherein said step of restraining includes inserting an insert into the valve and carrying out said step of subject¬ ing with the insert within the valve. -BU EA T OMPI 3. A method of tanning an animal aortic valve wherein the valve includes a tubular aorta seg¬ ment and valve leaflets wherein each of the valve leaf¬ lets is affixed to the aorta segment at a juncture, said method comprising: inserting an insert having a leading end into the tubular aorta segment to a location in which the leading end is adjacent the junctures; subjecting the valve to a tanning fluid at a differential pressure across the valve leaflets in the range of greater than zero and less than about four millimeters of mercury with the differential pressure being in a direction to urge the valve leaflets closed and with the tanning fluid tending to distort the valve; and carrying out said step of subjecting for a sufficient length of time to obtain fixation of the valve with the insert at least assisting in restrain¬ ing the valve against said distortion. 4. A method as defined in claim 3 wherein first and second of the valve leaflets are adjacent with adjacent edges of the first and second valve leaflets defining a commissure and said insert has first and second projections separated by a recess, and said step of inserting is carried out such that in said location the first and second projections are adjacent the first and second valve leaflets, respectively, and the commissure recess confronts the commissure. 5. A method as defined in -claim 3 wherein one of the valve leaflets is a right coronary valve leaflet, said insert has first and second circumfer¬ entially spaced regions with outer surfaces at said leading end, the outer surface of said first circum¬ ferentially spaced region lying more radially outwardly than the outer surface of the second circumferentially spaced region, and said step of inserting includes -in¬ serting the insert into the aorta segment such that at said location said first circumferentially spaced region is adjacent said right coronary valve leaflet. -BU E U OMPI 6. A method as defined in claim 3 wherein there are three of said valve leaflets with adjacent edges of the leaflets defining commissures and said insert is tubular and includes three projections with each adjacent pair of projections- being separated by a recess and with each of said projections terminating at said leading end in a curved end surface as viewed in side elevation and said step of inserting includes inserting the insert into the aorta segment such that at said location said curved end surfaces are adjacent the junctures of the three valve leaflets, respectively, said recesses generally confront the commissures, re¬ spectively, and said insert is out of contact with the valve leaflets. 7. A method as defined in claim 6 wherein the aorta segment has an aortic wall defining a sinus > of Valsalva adjacent each of the junctures, each of said end portion includes a segment projecting radial- ly outwardly, said segments in said location engaging the aortic wall at the sinuses of Valsalva, respectively. 8. A method as defined in claim 3 wherein said insert is tubular and said step of subjecting in¬ cludes introducing the tanning fluid to one side of the valve leaflets through the tubular insert. 9. A method of obtaining fixation of an animal heart valve wherein the heart valve includes a tubular segment and leaflets affixed to the tubular segment, said method comprising: subjecting the valve to a tanning fluid while maintaining a differential pressure across the valve leaflets in a direction to urge the valve leaf¬ lets closed wi_h such differential pressure being suf¬ ficient to maintain the valve leaflets closed and in- sufficient to provide significant irreversible altera¬ tions in the collagen waveform of the valve leaflets, the tanning fluid tending to distort the valve; carrying out said step of subjecting for a sufficient length of time to obtain fixation of the valve; and mechanically restraining the valve against substantial distortion during said step of subjecting. 10. A method as defined in claim 9 wherein said step of restraining includes inserting an insert within the valve and carrying out said step of subject¬ ing with the insert within the valve. - - 11. A method as defined in claim 10 wherein there are three of the valve leaflets with each adja¬ cent pair of the valve leaflets defining a commissure and with each valve leaflet being joined to the tubu- lar segment along a juncture, said insert is tubular and includes three projections with each adjacent pair of projections being separated by a recess, and said step of inserting includes inserting the insert into the tubular segment such that at said location said three projections are closely adjacent the junctures, respectively, said recesses generally confront the commissures, respectively, and the insert is out of contact with the valve leaflets. 'BUR AU- OMPI ,Δ > - wipo .»_ 12. An insert for use in restraining an ani¬ mal heart valve against distortion during fixation, said insert comprising: a tubular peripheral wall open at both ends and defining a generally axial passage; said peripheral wall having three generally axially extending projections, each of said projections terminating axially outwardly in a curved end surface as viewed in side elevation, each adjacent pair of said projections being circumferentially spaced by a recess ; each of said projections having an outer tis¬ sue supporting surface closely adjacent its free end, the outer tissue supporting surface of one of said projections lying more radially outwardly of the axis of said passage than the o.uter tissue supporting sur¬ faces of the other two projections whereby said outer tissue supporting surfaces are arranged eccentrically about the axis of said passage; and at least the exposed surface of said insert being constructed of a biocompatible material. gTjRE T OMPI l 13. An. insert as defined in claim 12 wherein each of said curved end surfaces is curved as viewed in axial cross section and said outer tissue supporting sur- faces lie farther radially outwardly than the peripheral wall adjacent the projections. 14. An insert as defined in claim 12 wherein each of said projections terminates in an end portion which includes a segment projecting radially outwardly, said outer tissue supporting surfaces being on said seg- ments, respectively. * 15. An insert as defined in claim 12 wherein the tubular wall is transparent. 16. An insert as defined in claim 14 wherein each of said segments includes a curved rib. "gU EA T OMP-

Inventors: LANE ERNEST, LANE, ERNEST
Applicants: AMERICAN HOSPITAL SUPPLY CORP, AMERICAN HOSPITAL SUPPLY CORPORATION
Application year: 1982
Main classification: C14C3
IPC codes: A61F2, A61L26, A61L27, C14B1, C14C3