COMPOUNDS AND METHODS FOR TREATING DEPRESSION

Abstract: Compounds of the formula: wherein: X is fluorine, chlorine, or bromine, and R is a group of the formula: wherein R₃ is hydrogen, (C₁-C₄)alkyl, or (C₁-C₄)alkoxy, and R₄ is (C₁-C₄)alkyl or (C₁-C₄)alkoxy; or a (C₁-C₈)alkyl ester thereof; or a non-toxic phar­ maceutically acceptable salt thereof; inhibit MAO in vivo and can be administered either alone or in combination with an aromatic L-amino acid decarboxylase inhibitor for the treatment of depression.

Description: Compounds and Methods for Treating Depression This invention relates to pharmacologicallyactive novel compounds, to methods o preparation of the compounds, and to pharmaceutical compositions containing the compounds. The class of compounds known as monoamine oxidase inhibitors (MAO inhibitors) has been employed in psychiatry for over 20 years for the treatment of depression E See Goodman and Gilnan, The Pharmacological Basis of Therapeutics, 6th end., McHillan Publishing Co., Inc., N.Y., 1980, pages 427 - 430 3 0 MAO Inhibitors currently used in the USA for treating depression are tranylcypromine (PANATE, SKF), phenelzine (NARDIL, Parke-Davis), and isocarboxazid (MARPLAN, Roche). In addition, another MAO inhibitor, pargyline (EUTRON, Abbott), is available for the treatment of hypertension E See Physicians' Desk Reference, 34th Ed., Medical Economics Co., Oradell, N.J., 1980, pages 1327 - 1328 (phenelzine), pages 1466 - 1468 (isocarboxazid), pages 1625 - 1630 (tranylcypromine), and pages 521 522 (pargyline) D. MAO Inhibitors can also be employed to treat other psychiatric disorders, such as phobic anxiety states. it is beiieved that the MAO inhibitors act to alleviate psychiatric disorders, such as depression, by increasing the concentration of one or more biogenic monoamines in the central nervous system. The monoamine oxidase enzyme (MAO) plays an important role in the metabolic regulation of the monoamines since it catalyzes the biodegradation of the monoamines through oxidative deamination. By inhibiting MAO, the degradation of the monoamines is blocked and the result is an increase in the availability of the monoamines for their physiological functions. Among the physiologically active monoamines which are known substrates for MAO are: (a) so-called " neurotransmitter tl monoamines, such as the catecholamines (e.g. dopamine, epinephrine, and norepinephrine) and the indoleamines (e.g. tryptamine and 5-hydroxytryptamine), (b) the so-called " trace " amines (e.g. o-tyramine, phenethylamine, tele-N-methylhistamine), and (c) tyramine. The usefulness of the MAO inhibitors in treating depression has been limited because the administration of such agents can potentiate the pharmacological actions of certain food substances or drugs leading to dangerous and sometimes lethal effects. For example, persons receiving a MAO inhibitor must avoid the ingestion of foods which have a high tyramine content (such as cheese) because the MAO inhibitor will block the metabolic degradation of tyramine in the gut and liver resulting in high circulating levels of tyramine, consequent release of catecholamlnes in the periphery, and finally serious hypertension. The potentiation by a MAO inhibitor of the pressor effect of tyramine arising from the ingestion of cheese, and the hypertensive episode produced thereby, are commonly known as the ' cheese reaction " or " cheese effect ". Moreover, persons on conventional MAO therapy can not be given directly-acting sympathomietic drugs (or precursors thereof) which are themselves substrates for MAO (e.g. dopamine, epinephrine, norepinephrine, or L-dopa) and of indirectly-acting sympathomimetic drugs (e.g. amphetamines or over-the-counter cold, hay-fever, or weight control preparations which contain a vasoconstrictor). The potentiation of the pressor effects of indirectly-acting sympathomimetic drugs is especially profound. This is because such drugs act peripherally primarily by releasing catecholamines in nerve endings, and the concentration of the liberated catecholamines will be dangerously elevated if the metabolic degradation of the catecholamines via MAO is blocked. In addition, a particular MAO inhibitor should not be used in combination with another MAO inhibitor or with hypotensive agents, dibenzapine antidepressants, meperidine, CNS depressants, and anticholinergic agents. Biochemical and pharmacological studies indicate that the MAO enzyme exists in two forms known as " MAO Type A " (MAO-A) and " MAO Type B " (MAO-B). The forms differ in their distribution in body organs, in their substrate specificity, and in their sensitivity to inhibitors. In general, MAO-A selectively oxidizes the so-called "neurotransmitter" monoamines (epinephrine, norepinephrine, and 5 hydroxytryptamine) while MAO-B selectively oxidizes the " trace " monoamines (o-tyramine, phenethylamine, and tele-N-methylhistamine). Both MAO-A and MAO-B oxidize tyramine, tryptamine, and dopamine. However, in man, dopamine has been shown to be a preferred substrate for MAO-B. The forms also differ in their sensitivity to inhibition, and thus, can be preferentially-inhibited depending upon the chemical structure of the inhibitor and/or the relative concentrations of the inhibitor and the enzyme. The MAO inhibitors currently sold in the USA for the therapy of depression (tranylcypromine, phenelzine, and isocarboxazid) are not preferential in their action upon MAO. However, various chemical compounds are known in the art to be preferential inhibitors of MAO, the most important being clorgyline, pargyline, and L-deprenyl which are all reported to be clinically effective antidepressant agents. MAO-A is preferentially inhibited by clorgyline, while MAO-B is preferentially inhibited by pargyline and Ldeprenyl. It should be observed that the "selectivity" of a MAO inhibitor arises because the inhibitor has a greater affinity for one form of the enzyme. Thus, the selectivity of a MAO inhibitor for MAO-A or MAO-B in vivo will be dose-dependent, selectivity being lost as the dosage is increased. Clorgyline, pargyline, and L-deprenyl are selective Inhibitors at lower dosages, but are not selective inhibitors at higher dosages. The literature concerning MAO-A and MAO-B and the selective inhibition thereof is extensive E See, for example, Goodman and Gilman, ibid, pages 204 - 205; Neff et al, Life Sciences, 14, 2061 (1974); Murphy, Biochemical Pharmacology, 27, 1889 (1978); Knoll, Chapter 10, pages 151 - 171 and Sandler, Chapter 11, pages 173 - 181, in Enzyme Inhibitors as Drugs, M. Sandler Ed., McMillan Press Ltd., London, 1980; Lipper et al., Psychopharmacology, 62, 123 (1979); Mann et al., Life Sciences, 26, 877 (1980); and various articles in Monoamines Oxidase: Structure, Function, and Altered Functions, T. Singer et al. Ed., Academic Press, N.Y., 1979 3. Of the selective inhibitors of MAO, L-deprenyl is of interest since the " cheese effect " is not observed at the low dosages where preferential inhibitions of MAO-B occur E See Knoll, TINS, pages 111 - 113, May 1979run. This observation is not unexpected since the intestinal mucosa contains predominantly MAO-A which, because it is not inhibited, permits cxidation and removal of the ingested tyramine. The selectivity of L-deprenyl for MAO-B may account for its ability to potentiate Ldopa for the treatment of Parkinson's disease without producing peripheral side effects, such as hypertension due to potentiation of pressor catecholamines E See Lees et al., Lancet, pages 791- 795, October 15, 1977 and Birkmeyer, Lancet , pages 439 - 443, February 26, 1977 3. In a compound aspect, the invention comprehends chemical compounds of the formula: EMI6.1 EMI7.1 wherein: X is fluorine, chlorine, or bromine; R is the group R2 as defined below; R1 is a group of the formula: EMI7.2 or the group N as defined below; wherein R2 is: EMI7.3 EMI8.1 wherein R3 is hydrogen, (C1-C4)alkyl, or (; -C4)- alkoxy, and R4 is (C1-C4)alkyl or (C1-C4)alkoxy; or a (C1-C8)alkyl ester thereof; or a non-toxic pharmaceutically acceptable salt thereof. The term " (C1-C4)alkyl " contemplates the methyl, ethyl, propyl, iso-propyl, n-butyl, tertbutyl, and iso-butyl groups. Methyl is preferred. The term " (C1-C4)alkoxy " contemplates the methoxy, ethoxy, n-prcpoxy, iso-propoxy, iso-butoxy, tertbutoxy, and n-butoxy groups. Methoxy is preferred. The (C1-C8)alkyl esters of the compounds of Formula I, TI, or III are those wherein the alkyl group forming the ester with the carboxyl group is either straight or branched and contains from 1 to 8 carbons. Examples of esters are methyl, ethyl, propyl, iso-propyl, n-butyl, iso-butyl, tert-butyl, n-hexyl, n-heptyl, or n-octyl. The methyl ester is preferred. The ester function must be chosen so that it is capable of being removed to form the free acid in vivo. Tt will be understood that when R2 is a phenyl group containing one or two -OH groups in combination with other substituents, such as the following phenyl groups: EMI9.1 the substituents (represented by R3 or Rg) may be located at any of the available positions of the phenyl ring. Illustrative examples of such substituted phenyl groups are: EMI10.1 wherein R4 is (C1-C4)alkyl, (C1-C4)alkoxy, or fluorine. Preferably R is methyl, ethyl, methoxy, or ethoxy. Methyl is most preferred. Suitable non-toxic pharmaceutically acceptable salts of the compounds cf Formula I, II, or III are known in the art and include acid addition salts formed by protonation of the amino group and salts formed by neutralization of the carboxylic acid function. As with any amino acid, the compounds may exist in the form of a zwitterion. Examples of acid addition salts are those formed from the following acids: hydrochloric, hydrobromic, sulfonic, sulfuric, phosphoric, nitric, maleic, fumaric, benzoic, ascorbic, pamoic, succinic, methanesulfonic, acetic, propionic, tartaric, citric, lactic, malic, mandelic, cinnamic, palmitic, itaconic, and benzenesulfonic. Examples of salts formed by neutralization of the carboxylic acid are metallic salts (e.g. sodium, potassium, lithium, calcium, or magnesium) and ammonium or (substituted) ammonium salts. The potassium and sodium salts are preferred. Preferred classes of compounds are: (i) compounds of Formula II or III wherein R1 is 3-hydroxyphenyl. (ii) compounds of Formula I, II, or III wherein P is EMI12.1 wherein PS is hydrogen, (C1-C4)alkyl, or (C1-C4)alRoxy and R4 is (C1-C4)alkyl or (C1-C 4)alkoxy, (iii) compounds as defined in (ii) wherein R3 is hydrogen. (iv) compounds as defined in Formula I, II, or III wherein X is fluorine. (v) compounds as defined in (i), (ii), and (iii) wherein X is fluorine. Preferred embodiments of the compounds of the invention are: 2-amino-3-(3'-hydroxy-4'-methylphenyl)-3-butenoic acid, 2-amino-4-fluoro-3- (2 '-hydroxyphenyl ) -3-butenoic acid, 2-amino-4-fluoro-3-(3'-hydroxyphenyl)-3-butenoic acid, 2-amino-4-fluoro-3-(2',3'-dihydroxyphenyl)-3- butenoic acid, 2-amino-4-fluoro-3-(2',4'-dShydroxyphenyl)-3- butenoic acid, 2-amino-4-fluoro-3-(2',5'-dihydroxyphenyl)-3- butenoic acd 2-amino-4-fluoro-3-(2',6'-dihydroxyphenyl)-3- butenoic acid, 2-amino-M-fluoro-3-(3',4'-dShydroxyphenyl)-3- butenoic acid, 2-amino-4-fluoro-3-(3' '5' -dihydroxyphenyl )-3- butenoic acid, 2-amino-4-fluoro-3-(3'-hydroxy- A I -methylphenyl)-3- butenoic acid. The compounds of Formula I, II, or III are in vivo precursors (or " prodrugs ") of certain substances which are irreversible inhibitors of MAO, and said compounds are useful in psychiatry for the treatment of depression. The compounds of Formula I, II, or III are not irreversible inhibitors of MAO in vitro. In order to produce irreversible inhibition of MAO in vivo and to exert their antidepressant effect, the compounds of Formula I, II, or III must be transformed into active metabolites which are the 2phenylallylamine compounds shown below respectively in Formula IV, V, or VI: EMI14.1 wherein X, R, and S have the meanings defined supra. The in vivo transformation of the compounds of Formula I, II, or III to the active metabolites of Formula IV, V, or VI occurs through a decarboxylation reaction catalyzed by an enzyme known as " aromatic L-amino acid decarboxylase " (AADC). AADC is known to decarboxylate various biologically important amino acids (such as dopa, tyrosine, phenylalanine, tryptophan, and 5-hydroxytryptophan) to form the corresponding monoamines. The antidepressant compounds of Formula IV, V, or VI which inhibit MAO in vitro and in vivo are described and claimed in the pending U.S. application of P. Bey entitled " Allylamine MAO Inhibitors ", Serial No. 268,555 filed June 1, 1981. It is known that AADC is present both in the brain and extracerebral tissue. Thus, the decarboxylation of a compound of Formula I, II, or III can take place both in the. brain and in extracerebral tissue with consequent inhibition of MAO. By administering a compound of Formula I, II, or III in combination with a compound capable cf preferentially blocking extracerebral AADC, the decarboxylation reaction producing the active metabolite will take place primarily in the brain, and hence primarily the brain MAO will be inhibited. The administration of a compound of Formula I, II, or III in combination with a peripheral AADC inhibitor for the treatment of depression, therefore, offers the advantages of'substantially avoiding the " cheese e-ffect " and other peripheral complications that are commonly associated with conventional MAO inhibitor therapy. In combination with an extracerebral AADC inhibitor, the compounds of Formula I, II, or III will provide a " site-directed " inhibition of MAO, the inhibition being confined primarily to the brain which has high AADC activity. Suitable AADC inhibitors for use in combination with the compounds of Formula I, II, or III will be apparent to those skilled in the art. Both competitive and irreversible inhibitors can be used. At the dosages used, the AADC inhibitor must be capable of inhibiting AADC extracerebrally without substantially inhibiting AADC in the brain. Examples of AADC inhibitors for use in combination with a compound of Formula I, II, or III are carbidopa and benzerazide, compounds which also have been found useful for blocking the peripheral decarboxylation of exogenous L-dopa administered for the treatment of Parkinsonism ESee Chapter 21, especially pages 482 483, " The Pharmacological Basis of Therapeutics " Goodman and Gilman Ed., McMillan Publishing Co., Inc., N.Y., 6th Ed., 1980 3. Other examples of suitable AADC inhibitors are the 2-amino-2 (monofluoromethyl or difluoromethyl )-3-(monohydroxy- phenyl or dihydroxyphenyl)propionic acids and like compounds, which are described and claimed in the pending U.S. application of P. Bey and M. Jung entitled " ct-Halomethyl Amino Acids ", Serial No. 6/210,500 filed November 26, 1980. The aforesaid 2 halomethylated 2-amino-3-(substituted phenyl)propionic acids are also described in Belgian patents Nos. 868,881 and 882,105. Preferred compounds are 2amino-2-(monofluoromethyl or difluoromethyl)-3- (3',4'-dihydroxyphenyl)propionic acid, and the 2',3'or 2',5'-dihydroxypheny1 isomers thereof. The preparation of 2-amino-3-phenyl-3-butenoic acid and 2-aminc-3-(3'-hydroxyphenyl)-3-butenoic acid from methyl 3-phenyl-2-butenoate and methyl 3-(3'tetrahydropyranyloxyphenyl)-2-butenoate, respectively, is described by R. Chari in the Doctoral Dissertation entitled " Synthesis of B,y- Unsaturated Amino Acids as Potential Irreversible Enzyme Inhibitors " University of Detroit, 1979 (available in print from University Microfilm International, Ann Arbor, Mich.). The compounds of Formula I in general can be prepared according to the method of Chari, or a modification thereof, depending upon the type of substitution in the phenyl ring and the consequent sensitivity of the double bond to acid. The starting materials for the preparation of the compounds of Formula I are the alkyl 2-butenoate compounds of Formula VII: EMI17.1 wherein: Rb is (C1-C4)alkyl, and Ra is a group of the formula: EMI18.1 wherein: R3 and R4 have the meanings defined supra and B is a protecting group for an aromatic -OH group. The preferred starting materials of Formula VII are those wherein Rb is methyl, ethyl, or tert-butyl. The purpose of the group defined by Rb is to protect the carboxy group from unwanted side reactions during subsequent process steps. The group represented by B in the definition of Ra can be any group known to be useful in the art of chemistry for protecting an aromatic -OH group, which group is readily removable under conditions which will not cause side reactions (such as polymerization) involving the double bond. Suitable protecting groups are: tetrahydropyranyl, methoxymethyl, methoxyethoxy-methyl, tert-butyl, benzyl, or triphenylmethyl. Preferred protecting groups are those that can be removed under very mild conditions. The selection and utilization of a particular aromatic -OH protecting group are known per se in the art. In the method of Chari, a compound of Formula VII is brominated in carbon tetrachloride at -100C to form the corresponding alkyl dibromobutyrate compound of Formula VIII: EMI20.1 which upon treatment with ammonia in dimethyl sulfoxide (DMSO) affords an alkyl 2-amino-3-butenoate compound of Formula IX: EMI20.2 When the intermediate of Formula IX does not have a protected -OH group or an alkoxy group in the 2- and/or 4-position of the phenyl ring, the intermediate can be converted to a compound of Formula I in two stages: (a) treatment with an acid (preferably dilute hydrochloric acid or saturated ethereal hydrogen chloride) under mild conditions (a temperature of O to 250C) for up to 16 days under which conditions the aromatic -OH protecting group (B) is removed, and (b) treatment with a strong acid (6N hydrochloric acid) at reflux temperature under which conditions the ester group is hydrolyzed. When the intermediate of Formula IX has a protected aromatic -OH group or an alkoxy group in -the 2- and/or d-position of the phenyl ring, the intermediate can be converted to a compound of Formula I by a three-stage process which comprises: (a) alkaline hydrolysis (preferably with lithium hydroxide in dioxane/water) at ambient temperature to remove the ester alkyl group (R ), b (b) neutralization of the salt thus formed (to pH ca 4.0, preferably with dilute hydrochloric acid) to afford the corresponding free acid, and (c) treatment with an acid (preferably dilute hydrochloric acid or ethereal hydrogen chloride) under mild conditions (a temperature of O to 250C) for up to 16 hours to remove the aromatic -OH protecting group (B). When R in Formula IX is tert-butyl, steps (a) and b (b) can be omitted. With acid sensitive intermediates, the three step process is preferred because the use of basic conditions and/or mild acid conditions to hydrolyze the ester function and to remove the protecting groups will minimize the occurence of side reactions involving the double bond (e.g. polymerization) which reactiona are promoted under vigorous acidic conditions. A modification of the above-described method involves the intermediate preparation and isolation of an N-protected 2-amino-3-butenoate compound of Formula X: EMI22.1 wherein: R and R have the meanings defined supra, and a b B1 is a protecting group for a primary amino group. The group represented by B1 can be any group known in the art to be useful for protecting a primary amino group, which group is readily removable under acidic conditions which will not cause side reactions (such as polymerization) involving the double bond. The selection of a particular protecting group and its method of utilization are known in the art. Examples of suitable protecting groups for the amino group are: formyl, acetyl, trifluoroacetyl, phthalyl, tosyl, benzenesulfonyl, benzyloxycarbonyl, (substituted) benzyloxycarbonyl, (e.g. the p-chloro, 2-bromo, - nitro, p-methoxy, o-chloro, 2,4-dichloro, and 2,6dichloro derivatives), t-butyloxycarbonyl (Boc), tamyloxycarbonyl, isopropyloxycarbonyl, 2-(pbiphenyl)isopropyloxycarbonyl, allyloxycarbonyl, cyclopentyloxycarbonyl, cyclohexyloxycarbonyl, adamentyloxycarbonyl, phenylthiocarbonyl, or triphenylmethyl. The preferred o-amino protecting group is t-butyloxycarbonyl (Boc) which can be introduced by reaction with di-tert-butyl di carbonate. The intermediate for Formula X can be prepared by treating a compound of Formula IX, as prepared according to the above-described method of Chari, with di-tert-butyl dicarbonate in tetrahydrofuran at 600C for about 2 - 4 hours. The intermediate of Formula X, regardless of the type of substitution in the phenyl ring can be converted to a compound of Formula I by three-stage procedure which comprises: : (a) alkaline hydrolysis (preferably with lithium hydroxide in dioxane/water) at ambient temperature to remove the ester alkyl group (R ), b (b) neutralization of the salt thus formed (to pH ca 4.0, preferably with dilute hydrochloric acid) to afford the corresponding free acid, and (c) treatment with an acid (preferably dilute hydrochloric acid or ethereal hydrogen chloride) under mild conditions (a temperature of O to 250C) for up to 16 hours to remove the aromatic -OH protecting group (B) and the amino group (B1). When Rb in Formula X is tert-butyl, steps (a) and (b) can be omitted. The modified method, as described above, is especially preferred for making the compounds of Formula I which have the -OH group or an alkoxy group at the 2-position or 4-position of the phenyl ring. Use of the N-protected derivative of Formula X in the modified procedure rather than the free amine intermediate of Formula IX, avoids decomposition of the amino acid during the subsequent base hydrolysis step and aids in purification of the hydrolysis product. The compounds of Formula II can be prepared in manner known per se by the method depicted below: EMI24.1 EMI25.1 In the above reaction scheme, is a group of the formula: EMI25.2 or a group as defined by Ra in Formula VII; Rb is (C1-C4)alkyl; B1 is a protecting group for a primary amino group; and X is fluorine, chlorine, or bromine except that the protecting group (B), as defined by Rc or Ra, cannot be tetrahydropyranyl. In Step A, an alkyl 4-halo-2-butenoate compound of Formula XI is brominated in manner known per se, preferably in carbon tetrachloride at OOC, to afford an alkyl 2,3-dibromo-4-halobutenoate compound of Formula XII. In Step C, the compound of Formula XII is dehydrohalogenated in manner known per se, preferably by treatment with sodium hydride in tetrahydrofuran (THF) at reflux temperature, to give the alkyl 4-halo-2-bromo-2-butenoate compound of Formula XIII, which is then isomerized in manner known per se to the corresponding 3-butenoate compound of Formula XIV, preferably by treatment with lithium diisopropylamide in THF at -780C. In Step D, the 3-butenoate compound of Formula XIV is treated with ammonia, preferably in dimethylsulfoxide (DMSO), at ambient temperature to afford the alkyl 4-halo-2amino-3-butenoate compound of Formula XV, which in Step E is treated in manner known per se to form the N-protected derivative of Formula XVI. The conversion (Stet F) of the N-protected derivative of Formula XVI to a final product of Formula II can be accomplished by a three-stage procedure which comprises: (a) alkaline hydrolysis (preferably with lithium hydroxide in dioxane/water) at ambient temperature to remove the ester alkyl group (R ), b (b) neutralization of the salt thus formed (to pH ca 4.0, preferably with dilute hydrochloric acid) to afford the corresponding free acid, and (c) treatment with an acid (preferably dilute hydrochloric acid or ethereal hydrogen chloride) under mild conditions (a temperature of O to 250C) for up to 16 hours to remove the aromatic -OH protecting group (B) and the a-amino group (B1). When Rb in Formula XVI is tert-butyl, steps (a) and (b) can be omitted. When it is desired to prepare a compound of Formula II in which the -OH group is present at the 3-position of the phenyl ring, and a -OH or alkoxy is not present in the 2- or 4-position of the phenyl ring, a further modified procedure can be used. This procedure is similar to that described above for preparing the compounds of Formula II, except that the starting material is a compound of Formula XI in which the aromatic -OH protecting group (B), as defined by Ra can also be a (C1 -C 4) straight chain alkyl group. The preparation is carried out in a manner similar to that described above for Steps A, B, C, D, and E. However, the N-protected derivative formed in Step E can be converted to a compound of Formula I in one step by treatment with 47 % hydrobromic acid at reflux temperature. Such treatment will remove the aromatic -OH protecting group (B), remove the ester alkyl function (Rb), and remove the a-amino protecting group (B1). Alternatively the N-protected derivative can be treated with dilute hydrochloric acid or saturated ethereal hydrogen chloride to remove the amino protecting group before treatment with 47 % hydrogen bromide. In Step E, the preferred N-protecting group is tert-butoxycarbonyl (Boc) which can be introduced into a compound of Formula XV in manner known per se, such as by reaction with di-tert-butyl dicarbonate. The compounds of Formula III can be prepared in a manner similar to that described for preparing the compounds of Formula II using as the starting material a compound of Formula XVII: EMI28.1 wherein Rb,R and X have the meanings defined supra with respect to Formula XI. The starting material of Formula VII, XI, or XVII can be prepared in known manner by the Wittig reaction by treating a ketone of the Formula: EMI28.2 with a suitable trialkylphosphonoacetate in dimetoxyethane (DME) at OOC in the presence of sodium hydride. The ketones of Formula XVIII, XIX, or XX are either known compounds or they can be prepared from known compounds by methods known in the art or obvious modifications thereof. For example, the compounds of Formula XVI II can be halogenated in known manner to prepare the compounds of Formula XIX or XX or an appropriately substItuted benzene compound can be acylated using a -riedel-Craft reaction. As will be appreciated by those skilled in the art, the compounds of Formula I, ZI, or III have an aromatic -OH group and an alpha-NH2 group, one or both of which may be acylated in manner known per se. It is known on the art that the N-acyl or O-acyl groups derived from an alkanoic acid or a naturally occurring amino acid can be removed to generate the free -NH group or -OH group in vivo. Thus, the acyl 2 derivatives can also be employed for the purposes of this invention, provided that the acyl group can be removed in vivo to give the desired amino acid. It will also be recognized that certain other derivatives can be converted in vivo to generate a free aromatic hydroxy or amino group. An example of such a derivative is 2-amino-4-fluoro-3-(3',4'methylenedioxyphenyl)-3-butenoic acid. It will also be apparent that certain derivatives of the carboxylic acid function1 other than esters and salts, can be employed for the purposes of this invention. Examples are primary amides, secondary or tertiary alkyl amides, and amides formed from the alpha- or terminal NH2- group of natural amino acids, since it is known in the art that the amide bond can be cleaved in vivo. Since the compounds of Formula I, II, or III possess an asymetric carbon atom enantiomers are possible, and the compounds of the inventIon may be in the form of the biologically active enantiomer or the racemate. The compounds of Formula I, II, or III may be obtained in the form of a pure enantiomer either by resolving a desired racemic product or by resolving a racemic starting material or intermediate at any convenient stage of the synthesis. Methods of carrying out the resolution are well known in the art of chemistry. When dosage ranges are given herein, they are applicable to the racemate. In addition, the compounds of Formula II can exist in forms wherein the substituents represented by X can be either cis or trans to the group represented by R1 . It is understood that the compounds of the invention may exist as the pure cis or trans form, or as mixtures thereof. The structural formula cf 2-amino-3-(3' hydroxyphenyl)-3-butenoic acid and 2-amino-3-(3' ,4'- dihydroxyphenyl)-3-butenoic acid are shown below in Formula XXI and XXII, respectively: EMI31.1 The (Cl-C8)alkyl esters and the pharmaceutically 18 acceptable salts of compounds XXI and XXII can be made by conventional methods. Suitable non-toxic, pharmaceutically acceptable salts are known in the art and include acid addition salts formed by protonation of the amino group and salts formed by neutralization of the carboxylic acid function. As with any amino acid, the compounds may exist in the form of a zwitterion. Examples of acid addition salts are those formed from the following acids: hydrochloric, hydrobromic, sulfonic, sulfuric, phosphoric, nitric, maleic, fumaric, benzoic, ascorbic, pamoic, succinic, methanesulfonic, acetic, propionic, tartaric, citric, lactic, malic, mandelic, cinnamic, palmitic, itaconic, and benzenesulfonic. Examples of salts formed by neutralization of the carboxylic acid are metallic salts (e.g. sodium, potassium, lithium, calcium, or magnesium) and ammonium or (substituted) ammonium salts. The potassium and sodium salts are preferred. Compounds XXI and XXII are metabolic precursors (or " prodrugs ") of substances which are irreversible inhibitors of MAO, and said compounds are useful in psychiatry for the treatment of depression. Compounds XXI and XXII are not irreversible inhibitors of MAO in vitro. In order to produce irreversible inhibition of MAO in vivo, and to exert their antidepressant effect, compounds XXI and XXII must be transformed into active metabolites which are the 2-phenylallylamine compounds shown below respectively in Formula XXIII and XXIV: : EMI32.1 The in vivo transformation of compounds XXI or XXII to the active metabolites of Formula XXIII or XXIV occurs through a decarboxylation reaction catalyzed by an enzyme known as " aromatic-L-amino acid decarboxylase " (AADC). AADC is known to decarboxylate various biologically important amino acids (such as dopa, tyrosine, phenylalanine, tryptophan, and '-hydroxytryptophan) to form the corresponding monoamines. The antidepressant compounds of Formula XXIII or XXIV, which inhibit MAO in vitro and in vivo, are described and claimed in the pending U.S. application of I. McDonald and M. Palfreyman entitled " Method for Treating Depression ", Serial No. 268,554 filed June 1, 1981. It is known that AADC is present both in the brain and extracerebral tissue. Thus, the decarboxylation of compounds XXI or XXII can take place both in the brain and in extracerebral tissue with consequent inhibition of MAO. By administering compounds XXI or XXII in combination with a compound capable of preferentially blocking extracerebral AADC, the decarboxylation reaction producing the active metabolite will take place primarily in the brain, and hence primarily the brain MAO will be inhibited. The administration of compound XXI or XXII in combination with a peripheral .MADC inhibitor for the treatment of depression, therefore, offers the advantages of substantially avoiding the " cheese effect " and other peripheral complications that are commonly associated with conventional MAO inhibitor therapy. In combination with an extracerebral AADC inhibitor, compound XXI or XXII will provide a " site-directed " inhibition of MAO, the inhibition being confined primarily to the brain which has high AADC activity. Suitable ASDC inhibitors for use in combInation with the compounds of Formula XXI and XXII will be apparent to those skilled in the art. Both competitive and irreversible inhibitors can be used. At the dosages used, the AADC inhibitor must De capable of inhibiting AADC extracerebrally without substantially inhibiting AADC in the brain. Examples of AADC inhibitors for use in combination with a compound of Formula XXI and XXII are carbidopa and benzerazide, compounds which also have been found useful for blocking the peripheral decarboxylation of exogenous L-dopa administered for the treatment of Parkinsonism See Chapter 21, especially pages 482 483, " The Pharmacological Basis of Therapeutics ", Goodman and Gilman end., McMillan Publishing Co., Inc., N.Y., 6th Ed., 1980 7 . Other examples of suitable AADC inhibitors are the 2-amino-2 (monofluoromethyl or difluoromethyl)-3 (monohydroxyphenyl or dihydroxyphenyl)propionic acids and like compounds, which are described and claimed in the pending U.S. application of P. Bey and M. Jung entitled " a-Halomethyl Amino Acids ", Serial No. 6/210,500 filed November 26, 1980. The aforesaid 2halomethylated 2-amino-3-(substituted phenyl)propionic acids are also described in Belgian patents Nos. 868,881 and 882,105. Preferred compounds are 2amino-2-(monofluoromethyl or difluoromethyl )-3- (3' ,4'-dihydroxyphenyl)propionic acid, and the 2',3'or 2' , 5' -dihydroxyphenyl isomers thereof. The preparation of 2-amino-3-(3 '-hydroxy- phenyl)-3-butenoic acid (XXI) from methyl 3-(3'hydropyranyloxyphenyl)-2-butenoate is described by R. Chari in the Doctoral Dissertataion entitled Synthesis of ssy-Unsaturated Amino Acids as Potential Irreversible Enzyme Inhibitors ", University of Detroit, 1979 (available in print from University Microfilm International, Ann Arbor, Mich.). In the method of Chari, methyl 3-(3'-tetra hydropyranyloxyphenyl ) -2-butenoate is brominated in carbon tetrachloride at -100C to form the corresponding dibromo compound which upon treatment with ammonia in dimethyl sulfoxide (DMSO) affords methyl 2-amino-3-(3 ' -tetrahydropyranyloxyphenyl)-3 butenoate. This intermediate can be converted to compound XXI in two stages: (a) treatment with saturated ethereal hydrogen chloride at ambient temperature, under which conditions the aromatic -OH protecting group is removed, and (b) treatment with 6N hydrochloric acid at reflux temperature under which conditions the ester group is hydrolyzed. A modification of the above-described method involves the preparation and isolation of ethyl 2 (tert-butoxycarbonylamino ) -3- (3 '-tetrahydropyranyl- oxyphenyl)-3-butenoate. This intermediate is prepared by treating methyl 2-amino-3-(3'-tetrahydropyranyl oxyphenyl)-3-butenoate, as prepared according to the above-described method of Chari, with di-tert-butyl di carbonate in tetrahydrofuran at 600C for about 2 4 hours. The intermediate can be converted to compound I by a three-stage procedure which comprises: (a) treating the N-tert-Boc derivative with lithium hydroxide in dimethoxyethane/water at ambient temperature to hydrolyze the ester function, (b) neutralizing the lithium salt then formed with dilute hydrochloric acid (to pH ca 4.0) to form the corresponding free acid, and (c) treating the free acid with saturated ethereal hydrogen chloride at O to 250C for about 16 hours too remove the the tert-Boc group. The modified procedure for preparing 2-amino-3 (3'-hydroxyphenyl)-3-butenoic acid is described in Example 13 herein. 2-AmTno-3-(3',4'-dlhydroxy- phenyl)-3-butencic acid (XXII) can be prepared from a suitably protected ethyl 3-(3' ,4'-dihydroxyphenyl)- 2-butenoate by following the general procedure of Example 13. However, the aromatic hydroxy protecting groups must be removed under very mild conditions to avoid the occurrence of side reactions involving the double bond. The Starting materials for the bromination reaction can be prepared in known manner by the Wittig reaction by treating the appropriate OH protected ketone with a suitable trialkylphosphonoacetate in DME at OOC in the presence of sodium hydride. The ketones are known compounds. Example 12 herein illustrates the preparation of ethyl 3-(3 ' -tetrahydropyranyloxy-phenyl)-2-butenoate from 3-tetrahydropyranyloxy-acetophenone. As will be appreciated by those skilled in the art, compounds XXI and XXII have an aromatic -OH group and an alpha-N group, one or both of which may be acylated in manner known per se. It is known in the art that the N-acyl or O-acyl groups derived from an alkanoic acid or a naturally occurring amino acid can be metabolically removed to generate the free -NH2 group or -OH group in vivo. Thus, the acyl derivatives can also be employed for the purposes of this invention, provided that the acyl group can be removed in vivo to give the desired amino acid. It will also be apparent that certain derivatives of the carboxylic acid function, other than esters and salts, can be employed for the purposes of this invention. Examples are primary amides, secondary and tertiary alkyl amides, and amides formed from the alpha- or terminal NH2- group of natural amino acids, since it is known in the art that the amide bond can be cleaved metabolically. Since compounds XXI and XXII possess an asymetric carbon atom, enantiomers are possible, and the compounds may be in the form of the biologically active enantiomer. or the racemate. The compounds may be obtained in the form of a pure enantiomer either by resolving a desired racemic product or by resolving a racemic starting material or intermediate at any convenient stage of the synthesis. Methods of carrying out the resolution are well known in the art of chemistry. When dosage ranges are given herein, they are applicable to the racemate. In a process aspect, the invention provides the following methods of preparation: (1) A method for preparing a compound of Formula I, II, or III which comprises hydrolyzing a compound of the formula: EMI39.1 wherein: X is fluorine, chlorine, or bromine, % is (c1-C4)alkyls R is the group R2, as defined below, E is the group R2, as defined below, or 2 group of the formula: EMI39.2 and R2, as defined by R or R1, above, is EMI40.1 wherein R3 is hydrogen, (91-C4)aikyl, or (i -C4)alkoxy, and R4 is (C1-C4)alkyl or (C1-C4)alkoxy; or an aromatic hydroxy-protected and/or aminoprotected derivative thereof; and, if necessary, removing in manner known per se the protecting group(s) either simultaneously with or subsequent to the hydrolysis reaction. (2) A method as defined in (1) above wherein said compound treated is an aromatic hydroxy-protected derivative. (3) A method as defined in (1) above wherein said compound treated is an amino-protected derivative. (4) A method as defined in (1) above wherein said compound treated is an aromatic hydroxy-protected. and amino-protected derivative. (5) A method as defined in (1) above wherein said compound treated is a compound of the formula: EMI41.1 wherein: Rb is (C -C )alkyl, 14 X is fluorine, chlorine, or bromine, and R1 is EMI42.1 wherein: R3 is hydrogen or (C1-C4)aikyi; or an aromatic hydroxy-protected and/or amino-protected derivative thereof. (6) A method as defined in (5) wherein X is fluorine and R3 is 3-hydroxyphenyl. (7) A method as defined in (6) wherein the compound treated is a (Cl-C4)alkyl ester of 2-amino-4-fluoro 3-(3'-hydroxyphenyl)-3-butenoic acid, or an aromatic hydroxy-protected and/or amino acid protected derivative thereof. (8) A method as defined in (5), (6), or (7) wherein the hydrolysis reaction is carried out using an aqueous strong acid at reflux temperature, under which conditions an aromatic hydroxy-protecting group or amino-protecting group is removed. (9) A method as defined in (8) wherein the hydrolysis is carried out using 47 % aqueous hydrogen bromide at reflux temperature. When employed to treat depression, the effective dosage of the compounds of Formula I, II, III, XXI, or XXII will vary according to the particular compound being employed, the severity and nature of the depression, and the particular subject being treated. In general, with the compounds of Formula I, XXI, or XXII, effective results can be achieved by the oral or parenteral route at a dosage level of from about 20 to about 200 mg per day, while with the compounds of Formula II and III, effective results can be achieved by the oral or parenteral route at a dosage level of from about 0.5 to about 50 mg per day. Therapy should be initiated at lower dosages, the dosage thereafter being increased until the desired effect is achieved. When an AADC inhibitor is co-administered with a compound of Formula I, II, III, XXI, or XXII, for the treatment of depression, the effective dosage of the AADC inhibitor must be capable of substantially blocking the AADC catalyzed decarboxylation of said compound extracerebrally without substantially blocking the AADC catalyzed decarboxylation in the brain. The effective dose will vary, however, according to the particular compound being employed and the dose of the antidepressant " prodrug administered. In general, with carbidopa and benzerazide effective results can be achieved by the oral or parenteral route at a dosage level of about 50 to 500 mg per day, preferably about 50 to 250 mg. With the 2-halomethylated 2-amino-3-(substituted phenyl)propionic acids described supra, effective results can be achieved by the oral or parenteral route at a dosage level of about 0.1 mg to 1000 mg per day. For example, with 2-amino-2-difluoromethyl 3-(3' ,4'-dihydroxyphenyl)propionic acid, and like compounds, the effective dose is about 10 to 1000 mg per day, preferably about 100 to 500 mg. With 2 amino-2-fluoromethyl-3-( 3' '4' -dihydroxyphenyl )- propionic acid, and like compounds, such as the 2,3dihydroxyphenyl isomer thereof, the effective dose is about 0.1 to 50 mg per day, preferably about 0.5 to 10 mg. It will be understood that the AADC inhibitor can be co-administered either substantially at the same time as or prior to the administration of a compound of Formula I, II, III, XXI, or XXII. When administered prior, the AADC inhibitor can be given up to 4 hours prior, depending upon the route of administration and severity of the conditions being treated. When used in combination with an AADC inhibitor, a compound of Formula I, II, III, XXI, or XXII and the AADC inhibitor can be administered separately, each being contained in a formulation in which the compound or the AADC inhibitor is the sole active agent or they can be administered together in a formulation containing both the compound and the AADC inhibitor as active agents. When both agents are contained in a single formulation, the relative amounts of each agent can vary depending upon the particular compounds employed. The compounds of this invention can be administered in various manners to achieve the desired effect. The compounds can be administrered alone or in combination with pharmaceutically acceptable carriers or diluents, the proportion and nature of which are determined by the solubility and chemical properties of the compound selected, the chosen route of administration, and standard pharmaceutical practice. The compounds may be administered orally in solid dosage forms, e.g. capsules, tablets, powders, or in liquid forms, e.g. solutions or suspensions. The compound may also be injected parenterally in the form of sterile solutions or suspensions. Solid oral forms may contain conventional excipients, for instance: lactose, succrose, magnesium stearate, resins, and like materials. Liquid oral forms may contain various flavoring, coloring, preserving, stabilizing, solubilizing, or suspending agents. Parenteral preparations are sterile aqueous or nonaqueous solutions or suspensions which may contain various preserving, stabilizing, buffering, solubilizing, or suspending agents. If desired, additives, such as saline or glucose, may be added to make the solutions isotonic. The amount of active compound administered will vary and can be any effective amount. Unit doses of these compounds can contain, for example, from about 1 mg to 100 mg of the compounds and may be administered, for example, one or more times daily, as needed. The term " unit dosage form " is used herein to mean a single or multiple dose form containing a quantity of the active ingredient in admixture or otherwise in association with the diluent or carrier, said quantity being such that one or more predetermined units are normally required for a single therapeutic administration. In the case of multiple dose forms, such as liquids or scored tablets, said predetermined unit will be one fraction such as 5 ml (teaspoon) quantity of a liquid or a half or quarter of a scored tablet, of the multiple dose form. In a composition aspect, the invention provides pharmaceutical compositions comprising a compound of the formula: EMI47.1 wherein: X is fluorine, chlorine, or bromine, and R5 is a group of the formula: EMI47.2 EMI48.1 wherein R3 is hydrogen, (C -C )alkyl, or (C -C )alkoxy, and 14 14 R 4 is (C1-C4)alkyl or (C -C )alkoxy; or a (C1-C8)alkyl ester thereof; or a non-toxic pharmaceutically acceptable salt thereof; in admixture with or otherwise in association with a pharmaceutically acceptable carrier or diluent thereof. In another composition aspect, there is provided a pharmaceutical composition comprising (a) a compound of Formula XXVI, XXVII, or XXVIII, wherein X and R are as defined hereinabove, and (b) an aromatic-L-amino acid decarboxylase inhibitor. Both active ingredients can be in admixture with or otherwise associated with a pharmaceutically acceptable carrier or diluent there for. Preferred aromatic-L-amino acid decarboxylase inhibitors are benzerazide, carbidopa, and a 2-amino-3-(monohydroxyphenyl or dihydroxyphenyl)propionic acid. The pharmaceutical formulations are prepared in a manner well known per se in the pharmaceutical art. The carrier or diluent may be solid, semi-solid, or liquid material which serves as a vehicle, excipient, or medium for the active ingredient. Suitable diluents or carriers are well known per se. The pharmaceutical formulations may be adapted for enteral or parenteral use and may be administered to the patient in the form of tablets, capsules, suppositories, solutions, suspensions, or the like. In the specific examples included herein-below, illustrative examples of suitable pharmaceutical formulations are described. The following examples are illustrative of the processes of the invention. All temperature are in centigrade. Example 1 a-Fluoro-3-methoxyacetophenone A solution of 3-methoxyacetophenone (6.0 g) in acetic acid (20 ml) is treated slowly with bromine (6.4 g) so that the temperature does not rise above 200. After the completion of the addition, the solution is stirred for 1 hour at 200, and the acetic acid is completely evaporated. The residue is crystallized from ethanol to give a-bromo-3methoxyacetophenone (7.51 g) as cream needles. A mixture of a-bromo-3-methoxyacetophenone (6.87 g) and potassium hydrogen fluoride (7.0 g) in diethylene glycol (50 ml) is heated (1000) and stirred for 5 hours. The mixture is cooled, poured into ice/water, and extracted with ether. The ether extract is washed consecutively with saturated aqueous sodium bicarbonate and water, dried, and evaporated to yield a solid mass. This is recrystallized from a mixture of ethanol and ether to afford pure a-fluoro-3-methoxyacetophenone (3.28 g): colorless needles, m.p. 53-540: NMR (CDCl3): 6 6 3.82, s, 3H; 5.43, d (J = 46 Hz), 2H; 6.93 to 7.53, m, 4H. Analysis for CgHgF02: Found : C, 64.59; H, 5.34 % Requires : C, 64.28; H, 5.39 % Example 2 Ethyl 3-(3'-methoxyphenyl-4 fluoro-2-butenoate A solution of dimethyl ethoxycarbonylmethane- phosphonate (-5.6 g) in dimethoxyethane (80 ml) is added dropwise to a cooled (00) suspension of sodium hydride (0.6 g) in dimethoxyethane (30 ml). The mixture is stirred at room temperature for 2 hours, and a solution of a-fluoro-3-methoxyacetophenone (4.20 g) in dimethoxyethane (100 ml) is added slowly. Stirring is continued for 30 minutes after the completion of the addition. The solution is cooled to about 100 and then poured into ice-water (200 ml) after which the mixture is extracted with ether. The ether solution is washed with water, dried, and evaporated to yield an orange oil (5.23 g). Purification is achieved by chromatography on silica gel (200 g) using light petroleum (90 %)/ether (10 %) as eluant whereupon there is obtained pure ethyl 3 (3'-methoxyphenyl)-4-fluoro-2-butenoate (3.92 g): almost colorless oil: NMR (CDC13): 6 1.28, t (J = 7 Hz), 3H; 3.77, s, 3H; 4.20, q (J = 7 Hz),; 5.83, d.d (J = 47 Hz, 1.5 Hz); 6.15, s (broad), 1H; 6.73 to 7.43, m, 4H. Analysis for C H FO Found : C, 65.53; H, 6.44 % Requires : C, 65.53; H, 6.35 % Example 3 Ethyl 2, 3-dibromo-3-( 3' -methoxyphenyl )- 4-fluorobutyrate To a solution of ethyl 3-(3'-methoxyphenyl)-4fluoro-2-butenoate (1.85 g) in carbon tetrachloride (40 ml) cooled to about -10 is added dropwise a solution of bromine (1.48 g) in carbon tetrachloride (10 ml). Stirring is continued at this temperature for 2 hours, after which the solution is evaporated to dryness. The residue is a pale orange oil (3.27 g) and is essentially pure ethyl 2,3-dibromo-3-(3' methoxyphenyl)-4-fluorobutyrate: NMR (CDC13): 6 1.23, t (J = 7-Hz), 3H; 3.75, s 3H; 4.20, q (J = 7 Hz), 2H; 5.00, s, 1H; 5.35, d (J = 50 Hz), 2H; 6.63 to 7.43, m, 4H. Example 4 Ethyl 2-bromo-3- (3 '-methoxyphenyl ) - 4-fluoro-2-butenoate A solution of ethyl 2,3-dibromo-3-(3'methoxyphenyl)-4-fluorobutyrate (5.55 g) in tetrahydrofuran (10 ml) is added dropwise to a suspension of NaH (0.41 g) in tetrahydrofuran (40 ml). The mixture is refluxed for 5 hours, cooled, and carefully treated with water. The mixture is then extracted with ether. The ether extract is washed with water, dried, and evaporated to leave an orange oil (4.52 g). Purification is achieved by silica gel (200 g) chromatography using as eluant a mixture of light petroleum (90 %)/ether (10 %) whereupon there is obtained pure ethyl 2-bromo-3-(3'-methoxyphenyl)4-fluoro-2-butenoate (3.85 g) almost colorless oil: NMR (CDCl3): 60.97, t (J = 7 Hz), 3H; 3.78, s, 3H; 4.00, q (J = 7 Hz), 2H; 5.35, d (J = 48 Hz), 2H; 6.70 to 7.52, m, 4H. Analysis for C13Hl4BrFO3: Found : C, 49.18; H, 4.35 % Requires : C, 49.23; H, 4.45 % Example 5 Ethyl 2-bromo-3-(3'-methoxyphenyl) 4-fluoro-3-butenoate A solution of ethyl 2-bromo-3-(3'methoxyphenyl)-4-fluoro-2-butenoate (3.80 g) in tetrahydrofuran (15 ml) is added to a cooled (-75 ) solution of lithium diisopropylamide prepared in the normal way from diisopropylamine (2.1 ml) and n butyllithium (10 ml of a 1.5 M solution in hexane) in tetrahydrofuran (60 ml). After the addition, the reaction mixture is stirred for 90 minutes at -75 . Then 5 % aqueous hydrochloric acid (10 ml), followed by water (20 ml), are added cautiously. The mixture is extracted with ether, and the ether extract is washed with dilute aqueous hydrochloric acid, then with water, dried, and evaporated to give 2-bromo-3 (3'-methoxyphenyl)-4-fluoro-3-butenoate (3.76 g) as a brown oil, and can be used without purification. Example 6 Ethyl 2-t-butoxycarbonylamino-3-(3 ' -methoxy- phenyl)-4-fluoro-3-butenoate A cooled (5 ) saturated solution of ammonia in dimethyl sulfoxide (15 ml) is added to ethyl 2 bromo-3- (3' -methoxyphenyl )-4-fluoro-3-butenoate (0.62 g) in a pressure vessel. The vessel is sealed and allowed to stand for 2 days at room temperature. The solution is then poured into ice-water and extracted with dichloromethane. The dichloromethane extract is washed thoroughly with water, dried, and evaporated to leave an orange oil (0.36 g). This substance is dissolved in tetrahydrofuran (30 ml) containing ditert-butyl dicarbonate (0.31 g) and then stirred at 600 for 2 hours. Evaporation of the solvent and purification of the residue (0.64 g) by silica gel (10 g) chromatography using light petroleum (90 %) /ether (10 %) as eluant allows the isolation of pure racemic ethyl 2-tert-butoxycarbonylamino-3-(3'methoxyphenyl)-4-fluoro-3-butenoate (0.22 g): colorless oil: NMR (CDCl3): 6 1.17, t (J = 7 Hz), 3H; 1.42, s, 9H; 3.72, s, 3H; 4.13, q (J = 7 Hz), 2H; 4.90, d (J = 8 Hz), 1H; 5.30, d (broad, J = 8 Hz), 1H; 6.85, d (J = 82 Hz), 1H; 6.70 to 7.40, m, 4H. Analysis for C18H24FNO5: Found : C, 61.22, H, 6.66; N, 3.87 % Requires : C, 61.18; H, 6.85; N, 3.96 % Example 7 Ethyl 2-amino-3-(3 ' -methoxyphenyl )- 4-fluoro-3-butenoate A solution of ethyl 2-tert-butoxycarbonylamino- 3-(3'methoxyphenyl)-4-fluoro-3-butenoate (218 mg) in ether (10 ml) saturated with dry HCl is left overnight by which time colorless needles crystallize. These are collected and dried to afford pure racemic ethyl 2-amino-3-(3'-methoxyphenyl)-4fluoro-3-butenoate (150 mg): colorless needles, m.p. 141-142 : Analysis for C H17 ClFNO3: Found : C, 54.22; H, 6.25; N, 4.67 % Requires : C, 53.89; H, 5.91; N, 4.83 % Example 8 2-Amino-3-(3'-hydroxyphenyl-4-fluoro 3-butenoic acid A solution of ethyl 2-amino-3-(3'-methoxyphenyl)-4-fluoro-3-butenoate (150 mg) in 47 % aqueous hydrobromic acid (10 ml) is refluxed for 3 hours after which the water is completely evaporated. The residue (orange oil, 112 mg) is dissolved in ethanol (1 ml) and treated with a slight excess of propylene oxide so that the pH is adjusted to about 4 to 5. The resulting precipitate is collected and subsequently recrystallized from ethanol/water whereupon pure racemic 2-amino-3-(3'-hydroxyphenyl)-4-fluoro-3butenoic acid (29 mg) is obtained: colorless needles, m.p. 2150: NMR (CD3OD): 6 4.62, s, 1H; 6.28 to 7.13, m, 4H; 6.98, d (J = 80 Hz), 1H. Analysis for Found : C, 56.73; H, 5.21; N, 6.49 % Requires : C, 56.87; H, 4.77; N, 6.63 % Example 9 Ethyl 2-bromo-3-(3'-methoxyphenyl) 4-fluoro-2-butenoate Piperidine (0.22 ml) is added to a solution of ethyl 2, 3-dibromo-3-(3 '-methoxyphenyl )-4-fluoro- butyrate (0.80 g) in ether (10 ml). The reaction mixture is stirred at room temperature for 3 hours and water is added. Ether extraction gives essentially pure ethyl 2-bromo-3-(3'-methoxyphenyl)4-fluoro-2-butenoate (0.65 g). Example 10 2-Amino-3-(3'-hydroxy-4'-methoxyphenyl)- 4-fluoro-3-butenoic acid Repeating the procedures described in Examples 2, 3, 9, 5, and 6, a-fluoro-3-tetrahydropyranyloxy- 4-methoxyacetophenone can be converted to ethyl 2 tert-butocycarbonylamino-3- (3 '-te trahydropyranyloxy- 4-methoxyphenyl)-4-fluoro-3-butenoate. The conversion of this intermediate to the final product is achieved as follows: A mixture of this product (0.45 g) and lithium hydroxide monohydrate (0.09 g) in water (3 ml) and THF (13 ml) is stirred at room temperature for 2 hours. Dilute aqueous hydrochloric acid is added to adjust the pH to about 4, then the acid is isolated by ether extraction. The crude material is treated overnight with a saturated solution of hydrogen chloride in ether (20 ml). Filtration affords a colorless powder (0.21 g) which is dissolved in ethanol (20 ml) and treated with propylene oxide (0.20 g) at room temperature overnight. Filtration of the precipitate affords 2-amino-3-(3'-hydroxy-4'- methoxyphenyl)-4-fluoro-3-butenoic acid (0.12 g) as a colorless powder, m.p. 2140: NMR (D20 + DCl): e 3.87, s, 3H; 5.00, s, 1H; 6.77 to 7.13, m, 3H; 7.25, d (J = 80 Hz), 1H. Analysis for C H FNO Found : C, 54.68; H, 5.18; N, 5.74 % Requires : C, 54.77; H, 5.01; N, 5.81 % Example 11 -Fluoro-3-tetrahydropyranyloxy- 4-me thoxyace tophenone A solution of 3-acetoxy-4-methoxyacetophenone (53 g) in chloroform (700 ml) is cooled in an ice bath and treated slowly with a solution of bromine (41 g) in chloroform (100 ml). The solution is then stirred for 90 minutes after which the chloroform is evaporated. The residue is dissolved in ether, washed in water, dried and evaporated. The solid residue is recrystallized from dichloromethane/hexane to afford v-bromo-3-acetoxy-4 methoxyacetophenone (59.51 g) as colorless plates: m.p. 116-1170. A portion (57.4 g) of this bromide, mixed with KH2F (46.8 g) in diethylene glycol (500 ml), is allowed to remain at 1000 for 8 hours. The mixture is cooled, poured into ice-water, and extracted with ether. The ether soluble material is purified by silica gel chromatography using light petroleum (50 %)/diethyl ether (50 %) as eluant whereupon a- fluoro-3-hydroxy-4-methoxyacetophenone (22.4 g) is obtained. Recrystallization from dichloromethane/ hexane affords colorless plates (20.2 g): m.p. 70710. The phenol (18.35 g) is treated with dihydropyran (20 ml) and concentrated hydrochloric acid (10 drops) at room temperature for 3 1/2 hours. The solution is poured into 2 % aqueous sodium hydroxide (300 ml) and extracted with ether. The ether soluble material is purified by silica gel chromatography (light petroleum (80 %)/diethyl ether (20 %)), and by recrystallization from dichloromethane/hexane to afford o-fluoro-3- tetrahydropyranyloxy-4-methoxyacetophenone (17.15 g), as colorless needles: m.p. 78-790: NMR (CDCl3): s 1.57 to 1.93, m, 6H; 3.43 to 4.10, m and 3.87, s, 5H; 5.40, d (J = 47 Hz), 2H; 5.40, s (broad), 1H; 6.80 to 7 63, ABC system, 3H Analysis for C H FO : 14 - 17 -- 4 Found : C, 62.59; H, 6.32 % Requires : C, 62.68; H, 6.39 % Example 12 Ethyl 3-(3 ' -tetrahydropyranyloxyphenyl)- 2-butenoate A solution of diethyl ethoxycarbonylmethanephosphonate (10.3 g) in dimethoxyethane (100 ml) is added dropwise to a cooled (00) suspension of sodium hydride (1.2 g) in dimethoxyethane (50 ml). When the addition is complete (about 30 minutes), the cooling bath is removed and the mixture is stirred for a further 2 hours. To this mixture is slowly added a solution of 3-tetrahydropyranyloxyphenylacetophenone (10.0 g) in dimethoxyethane (200 ml) at room temperature. The reaction mixture is then heated at about 700 for 4 hours, cooled to room temperature, and poured into water (1 1). Ether extraction followed by silica gel (200 g) chromatography (light petroleum (80 %)/ether (20 %) as eluant) enables the isolation of pure ethyl 3-(3'-tetrahydropyranyloxyphenyl)-2-butenoate (8.45 g): colorless oil: NMR (CDCl3): 6 1.27, t (J = 7 Hz), 3H; 1.40 to 2.07, m, 6H; 2.50, d (J = 1.5'Hz), 3H; 3.33 to 3.93, m, 2H; 4.13, q (J = 7 Hz), 2H; 5.35, s (broad), lH; 4.37, t (J = 1.5 Hz), 1H; 6.83 to 7.57, m, 4H. Example 13 Step A: Ethyl 2,3-dibromo-3-(3'-tetrahydro pyranyloxyphenyl)butyrate A solution of bromine (3.50 g) in carbon tetrachloride (60 ml) is added dropwise to a cooled (-10 ) solution of ethyl 3-(tetrahydropyranyloxyphenyl)-2-butenoate (5.80 g) and pyridine (10 drops) in carbon tetrachloride (60 ml). After the completion of the addition, the reaction mixture is stirred for 1 hour at about -100, and the solvent is evaporated to leave an orange oil (9.42 g). Chromatography on silica gel (200 g) using a mixture of light petroleum (80 %) and ether (20 %) as eluant allows the separation of pure ethyl 2,3-dibromo-3-(3'tetrahydropyranyloxyphenyl)butyrate (5.47 g): colorless oil: NMR (CDCl3): 6 1.37, t (J = 7 Hz), 3H; 1.27 to 2.20, m, 6H; 2.55, s, 3H; 3.40 to 4.13, m, 2H; 4.33, q (J = 7 Hz), 2H; 5.05, s, 1H; 5.10, s (broad), 1H; 6.73 to 7.43, m, 4H. Step B: (Racemic) Ethyl 2-(tert-butoxycarbonylamino) 3-(3'-tetrahydropyranyloxyphenyl)-3-butenoate A saturated solution of ammonia in dimethyl sulfoxide (20 ml) is added to ethyl 2,3-dibromo-3 (3'-tetrahydropyranyloxyphenyl)butyrate (1.5 g) in a suitable pressure vessel at about 5 . The reaction vessel is firmly sealed. The solution is left at ambient temperature for 40 hours and is then poured into ice-cold water (200 ml) which is extracted with dichloromethane. The organic extract is thoroughly washed with water, dried, and evaporated to leave a yellow oil (0.64 g). A solution of this material and di-tert-butyl dicarbonate (0.45 g) in tetrahydrofuran (50 ml) is heated at 600 for 2 hours. Evaporation to dryness leaves an orange oil (0.93 g). Purification is achieved by silica gel (30 g) chromatography using light petroleum (90 %)/ether (10 %) as eluant whereupon there is obtained racemic ethyl 2-(tert butoxyearbonylamino)-3-(3'-tetrahydropyranyloxy- phenyl)-3-butenoate (0.43 g): colorless oil: NMR (CDCl3): 61.08, t (J = 7 Hz), 3H; 1.37, s, 9H; 1.40 to 2.10, m, 6H; 3.30 to 3.90, m, 2H; 4.08, q (J = 7 Hz), 2H; 4.92 to 5.53, m, 5H; 6.77 to 7.33, m, 4H. Step C: (Racemi c) 2-(tert-Butoxycarbonylamino) 3-(3'-tetrahydropyranyloxyphenyl)-3 butenoic acid A solution of ethyl 2-(tert-butocycarbonyl amino) -3- (3 '-tetrahydropyranyloxyphenyl ) -3-butenoate (0.40 g) in dimethoxyethane (10 ml) and water (2 ml) is treated with solid lithium hydroxide monohydrate (0.02 g). After stirring for 2 hours at room temperature, the mixture is diluted with water and acidified with 0.1 N hydrochloric acid to ca pH 4. Extraction-with ether gives essentially pure racemic 2-(tert-butoxycarbonylamino)-3-(3 '-tetrahydro- pyranyloxyphenyl)-3-butenoic acid (0.25 g): colorless solid. Step D: Hydrochloride of (racemic) 2-amino-3 (3' hydroxyphenyl)-3-butenoic acid A solution of 2-(tert-butoxycarbonylamino)-3 (3'-tetrahydropyranyloxyphenyl)-3-butenoic acid (0.25 g) in ether (10 ml) saturated with dry hydrogen chloride is left standing at about 50 for 16 hours during which time colorless crystals precipitate. These are collected and dried to give the hydrochloride of racemic 2-amino-3-(3'-hydroxyphenyl)-3-butenoic acid (0.12 g): colorless needles, m.p. 199-200 : Analysis for C10H12C1N03: Found : C, 52.08; H, 5.24; N, 5.95 % Requires : C, 52.29; H, 5.27; N, 6.10 % Step E: (Racemic) 2-Amino-3-(3'-hydroxyphenyl) 3-butenoic acid A solution of the hydrochloride of 2-amino-3 (3'-hydroxyphenyl)-3-butenoic acid (0.12 g) in ethanol (2 ml) is treated with a slight excess of propylene oxide. After several hours, the precipitated material is collected and dried to afford pure racemic 2-amino-3-(3'-hydroxyphenyl-3butenoic acid: colorless powder, m.p. 204-205 : NMR (CD3OD): 6 4.8, s obscured by Cg OH peak; 5.33, s, 1H; 5.47, s, 1H; 6.47 to 7.13, m, 4H. In the following Examples relating to pharmaceutical compositions, the term " active compound " is used to indicate the compound 2-amino3-(3'-hydroxy)phenyl-3-fluoroallylamine. This compound may be replaced in these compositions by any other compound of the invention. Adjustments in the amount of medicament may be necessary or desirable depending upon the degree of activity of the medicament as is well known in the art. Example 14 Ar. illustrative composition for hard gelatin capsules is as follows: (a) active compound 0.5 mg (b) talc 5 mg (c) lactose 94.5 mg The formulation is prepared by passing the dry powders of (a) and (b) through a fine mesh screen and mixing them well. The powder is then filled into hard gelatin capsules at a net fill of 100 mg per capsule. Example 15 An illustrative composition for tablets is as follows: (a) active compound 0.5 mg (b) starch 48 mg (c) lactose 51.5 mg (d) magnesium stearate 2 mg The granulation obtained upon mixing the lactose with the compound (a) and the part of the starch and granulated with starch paste is dried, screened, and mixed with the magnesium stearate. The mixture is compressed into tablets weighing 100 mg each. Example 16 An illustrative composition for an injectable suspension is the following 1 ml ampul for an intramuscular injection: weight per cent (a) active compound 0.1 (b) polyvinylpyrrolidone 0.5 (c) lecithin 0.25 (d) water for injection to make 100 The materials (a) - (d) are mixed, homogenized, and filled into 1 ml ampuls which are sealed and autoclaved 20 minutes at 1210. Each ampul contains 1 mg per ml of active compound. Example 17 mg/suppository Active compound 1 Oil of Theobroma g99 The medicament is powdered and passed through a B.S. No. 100 sieve and triturated with molten oil of Theobroma at 450 to form a smooth suspension. The mixture is well stirred and poured into moulds each of nominal 1 G capacity, to produce suppositories. Example 18 2-Amino-3-(3'-hydroxyphenyl)-3-butenoic acid (herein-after referred to as " AHBA "), 2-amino-4 fluoro-3-(3 '-hydroxyphenyl )-3-butenoic acid (hereinafter referred to as " AFHBA "), and 2-amino-4fluoro-3-(3 ' -hydroxy-4-methoxyphenyl )-3-butenoic acid (herein-after referred to as " AFMBA ") were tested as follows: A. In vitro testing AHBA or AFHBA was incubated with partially purified hog kidney AADC at 370 for various times up to 2 hours. HPLC analysis determined that at 2 hours each compound (as the DL-mixture) underwent 50% decarboxylation to give the corresponding allylamine fl 2-(3 ' -hydroxy)phenylallylamine or 2-(3'-hydroxy)phenyl-3-fluoroallylamine, respectively] When the experiment was repeated in the presence of 10 M amonofluoromethyl-Dopa (MFMD) (an AADC inhibitor), no decarboxylation was observed. The decarboxylated products are time-dependent irreversible inhibitors of MAO in vitro: IC50 for 2 (3'-hydroxy)phenylallylamine, N 10 5. IC50 for 2 (3'-hydroxy)phenyl-3-fluoroallylamine, ¯ 10 . AHBA and AFHBA are inactive or very weak inhibitors of MAO. B. Ex vivo testing I. Mice were injected with either AHBA (250 mg/kg, i.p.) alone or with AHBA (250 mg/kg, i.p.) in combination with MFMD (1 mg/kg, i.p.) administered 30 minutes before AHBA. Mice were killed 4 hours later and MAO activity (tyramine as substrate) was determined in brain and heart. AHBA administered alone produced 30% inhibition of MAO in brain and heart. AHBA in combination with MFMD produced 65% inhibition of MAO in the brain. To determine the site-selective effect, MAO activity was determined in mouse brain using the neuronal substrate 5-HT and the non-neuronal substrate phenylethylamine (PEA). AHBA administered in combination with MFMD inhibited neuronal MAO (5-HT substrate) by 82% and non-neuronal MAO (PEA substrate) by 15%. II. Rats were injected with AFHBA (0.5 mg/kg, i.p.) alone or with AFHBA (0.5 mg/kg, i.p.) in combination with MFMD (2.0 mg/kg, i.p.) administered 30 minutes before AFHBA. Animals were killed 18 hours later and MAO activity (5-HT and phenethylamine as substrates) was determined in brain, heart, and liver. In the brain, AFHBA administered alone inhibited neuronal MAO by 72% and non-neuronal MAO by 37%. Pretreatment with MFMD did not essentially reduce the inhibition of neuronal MAO (68%), but it reduced inhibition of non-neuronal MAO -to 28%. In the heart, AFHBA inhibited neuronal MAO by 52% and non-neuronal MAO by 44%, but pretreatment with MFMD reduced neuronal inhibition of MAO to 18% and non-neuronal inhibition to 4%. In the liver, MAO inhibition by AFMBA alone was 29% (neuronal) and 38% (non-neuronal), but pretreatment with MFMD totally blocked MAO inhibition. When the above experiments with AFHBA were repeated using carbidopa (50 mg/kg, i.p.), the AADC inhibitor produced the same protective effect against inhibition of MAO in the heart as MFMD (2.0 mg/kg, i.p.). To demonstrate the actiyity of AFHBA by oral administration, rats were gavaged with various doses of the compounds and killed 18 hours later. MAO activity was determined in the brain using 5-HT and phenethylamine (PEA) as substrates. The following results were obtained: % Inhibition of MAO Dose (mg/kg) Neuronal Non-neuronal 0.5 63 41 1.0 71 64 2.5 95 83 III.Rats were injected with AFMBA (100 mg/kg, i.p.) and were killed 18 hours later. MAO activity was determined in the brain, heart, and liver using 5-HT and PEA. The following results were obtained: % Inhibition of MAO Neuronal Non-neuronal Brain 16 40 Heart 18 46 Liver 2 11 IV. Rats were injected with AHBA as the ethyl ester (334 mg/kg, i.p.) alone or in combination with carbidopa (100 mg/kg,i.p.) given 30 minutes before administration of the ethyl ester of AHBA. The animals were killed 4 hours later. MAO activity was determined in the brain using 5-HT and PEA as substrates. The following results were obtained: % Inhibition of MAO Brain Heart Liver Treatment N* Non-N* N* Non-N* N* Non-N* AHBA, ethyl 59 40 23 3 37 0 ester AHBA, ethyl 78 31 0 0 20 13 ester + carbidopa N* means Neuronal. C. In vivo testing Rats were injected with AFHBA (0.5 mg/kg, i.p.) alone or with AFHBA (0.5 mg/kg, i.p.) in combination with MFMD (2 mg/kg, i.p.) or carbidopa (50 mg/kg, i.p.) given 30 minutes before the AFHBA. The rats were killed 18 hours later, and dopamine, DOPAC, 5 HT, and 5-HIAA concentrations were determined in the brain. The following results were obtained: % Change from Control Treatment Dopamine DOPAC 5-HT 5-HIAA AFMBA + 25 - 76 + 30 - 34 AFHBA + MFMD + 50 - 92 + 92 - 48 AFHBA + + 54 - 89 + 92 - 37 carbidopa In a separate experiment, rats were given AFMBA orally at various doses. The. rats were killed 18 hours later and dopamine, DOPAC, 5-HT, and 5-HIAA concentrations were determined in the brain. The following results were obtained: % Change from Control Dose (mg/kg) Dopamine DOPAC 5-HT 5-HIAA 0.5 + 8 - 55 + 26 - 11 1.0 + 17 - 72 + 74 - 23 2.5 + 28 - 79 + 140 - 41

Claims: Claims 1. A compound of the formula: EMI73.1 wherein: X is fluorine, chlorine, or bromine; R is the group R2 as defined below; R1 is a group of the formula: EMI73.2 or the group N as defined below; wherein R2 is: EMI73.3 EMI74.1 wherein R3 is hydrogen, (C1-C4)alkyl, or ( -C4)- alkoxy, and R4 is (C1-C4)alkyl or (C1-C4)alkoxy:, or a (C1-C8)alkyl ester thereof; or a non-toxic pharmaceutically acceptable salt thereof. 2. A compound of Formula I as claimed in Claim 1. 3. A compound of Formula II as claimed in Claim 1. 4. A compound of Formula III as claimed in Claim 1. 5. A compound as claimed in any one of Claims 3 and 4 wherein R1 is: EMI75.1 6. A compound as claimed in any one of Claims 2 to 4 wherein E is: EMI75.2 wherein R3 is hydrogen, (C1-C4)alkyl, or (C1-C4)alkoxy, and R4 is (C1-C4)alkyl or (C1-C4)alkoxy. 7. A compound as claimed in Claim 6 wherein R3 is hydrogen. 8. A compound as claimed in any one of Claims 2 to 8 wherein X is fluorine. 9. 2-Am.ino-X-fluoro-3-(3'-hydroxyphenyl)-3- butenoic acid or a non-toxic pharmaceutically acceptable salt thereof. 10. 2-Amino-4-f'*oro-3-(3'-hydroxyphenyl)-3- butenoic acid, (C1-C8)alkyl ester. 11. 2-Amino-4-fluoro-3-(3' -hydroxyphenyl )-3- butenoic acid, methyl ester. 12. 2-Arnino-4-fluoro-3- (3 '-hydroxyphenyl )-3- butenoic acid, ethyl ester. 13. A compound as claimed in any one of the preceding claims for use in the inhibition in the human body of monoamine oxidase. 14. A compound as claimed in any one of the preceding claims for use in the therapy of depression in the human body. 15. A pharmaceutical composition comprising a compound as claimed in any one of the preceding claims in admixture or otherwise associated with a pharmaceutically acceptable carrier or diluent therefor. 16) A pharmaceutical composition as claimed in Claim 15 in unit dosage form containing 1 mg to iOC mg of said compound per unit dose. 17) A pharmaceutical composition comprising (a) a compound as elaimed in any one of Claims 1 to 14 and (b) an aromatic L-amino acid decarboxylase inhibitor. 18) A pharmaceutical composition as claimed in Claim a-ni 17 wherein the active ingredients are in admixture cr otherwise associated with a pharmaceuticall acceptable carrier or diluent therefor. 13) A Compound selected from 2-amino-3-(3'-hydroxy- phenyl)-3-butenoic acid, 2-amino-3-(3' ,4'-dihydroxyphenyl) -3-butenoic acid, a (Cl-C8)alkyl ester thereof, or a non-toxic pharmaceutical acceptable salt thereof for use in the therapy of depression in the human body. 20. A pharmaceutical composition comprising 2 amino-3-(3 ' -hydroxyphenyl)-3-butenoic acid, or a (Ci -C8)alkyl ester thereof, or a non-toxic pharmaceutically acceptable salt thereof, in admixture or otherwise associated with a pharmaceutically acceptable carrier or diluent therefor. 21. A pharmaceutical composition comprising 2 amino-3-(3' '4 '-dihydroxyphenyl )-3-butenoic acid, or a (C1-C8)alkyl ester thereof, or a non-toxic pharmaceutically acceptable salt thereof, in admixture or otherwise associated with a pharmaceutically acceptable carrier or diluent therefor. 22. A pharmaceutical composition comprising (a) 2 amino- (3 1-hydroxyphenyl ) -3-butenoic acid, or a (C-C8)alkyl ester thereof, or a non-toxic pharmaceutically acceptable salt therof, and (b) an aromatic L-amino acid decarboxylase inhibitor. 23. A pharmaceutical composition comprising (a) 2amino-(3',4' -dihydroxyphenyl )-9-butenoic acid, or a (t -C8')alkyl ester thereof, or a non-toxic pharmaceutically acceptable salt thereof, and (b) an aromatic L-amino acid decarboxylase inhibitor. 24. A pharmaceutical composition as claimed in any one of Claims .22 and 25 wherein the active ingredients are in admixture or otherwise associated with a pharmaceutically acceptable carrier or diluent therefor. 25. A pharmaceutical composition as claimed in any one of Claims 17, 18, 22, 23, and 24 wherein the aromatic L-amino acid decarboxylase inhibitor is carbidopa, benzerazide, or a 2-amino-2-(monofluoromethyl or difluoromethyl)-3-(monohydroxyphenyl or dihydroxyphenyl)propionic acid. 26. A process of preparing a compound as claimed in Claim 1 which comprises hydrolyzing a compound of the formula: EMI79.1 wherein: R, R1, and X are as defined in Claim 1 and Rb is (C -C4)alkyl; 14 or an aromatic hydroxy-protected and/or aminoprotected derivative thereof; and, if necessary, removing in manner known per se the protecting group(s) either simultaneously with or subsequent to the hydrolysis reaction. CLAIMS FOR AUSTRIA 1) A process for preparing a compound of the formula: EMI81.1 wherein: X is fluorine, chlorine, or bromine; R is the group Rê as defined below; R1 is a group of the formula: EMI81.2 or the group N as detined below; wherein R2 is: EMI81.3 EMI82.1 wherein R3 is hydrogen, (Cl-C4)alkyl, or (9L-C4)- alkoxy, and R4 is (C1-C4)alkyl or (C1-C4)alkoxy;, or a (c1-C8)alkyl ester thereof; or a non-toxic pharmaceutically acceptable salt thereof which comprises hydrolyzing a compound of the formula EMI82.2 wherein: R, R1, and X are as defined above and Rb is (C -C )alkyl; 14 or an aromatic hydroxy-protected and/or aminoprotected derivative thereof; and, if necessary, removing in manner known per se the protecting group(s) either simultaneously with or subsequent to the hydrolysis reaction. 2. A method as claimed in Claim l wherein said compound treated is an aromatic hydroxy-protected derivative. 3. A method as claimed in Claim l wherein said compound treated is an amino-protected derivative. 4. A method as claimed in Claim 1 wherein said compound treated is an aromatic hydroxy-protected and amino-protected derivative. 5. A method as claimed in Claim 1 wherein said compound treated is a compound of the formula: EMI83.1 wherein: is is (i -C)alkyl, X is flucrine, chlorine, or bromine, and RI is EMI84.1 wherein R is hydrogen or (C1-C4)alkyl; or an aromatic hydroxy-protected and/or amino-protected derivative thereof. 6. A method as claimed in Claim 5 wherein said compound treated is a (C1-C4)alkyl ester of 2amino-4-fluoro-3-(3 '-hydroxyphenyl)-3-butenoic acid. 7. A method as claimed in Claim 5 wherein said compound treated is an aromatic protected derivative of a (C1-C4)alkyl ester of 2-amino-4-fluoro-3-(3' hydroxyphenyl )-3-butenoic acid. 8. t method as claimed in Claim 5 wherein said compound treated is an amino-protected derivative of a (C1-C4)alkyl ester of 2-amino-4-fluoro-3-(3'hydroxyphenyl )-3-butenoic acid. 9. A method as claimed in Claim 5 wherein said compound treated is an aromatic hydroxy-protected and an amino-protected derivative of a (i -C4)alkyl ester of 2-amin-4-fluoro-3-(3'-hydroxyphenyl)-3- butenoic acid. 10.A method as claimed in any one of Claims 1 to 9 wherein the hydrolysis reaction is carried out using an aqueous strong acid at reflux temperature. 11 A method as claimed in Claim 10 wherein the hydrolysis reaction is carried out using 47% aqueous hydrogen bromide at reflux temperature. 12. A process as in claim 1 for preparing 2-amino -3-(3 '-hydroxyphenyl)-3-butenoic acid, 2-amino -3-(3',4'-dihydroxyphenyl)-3-butenoic acid, a (Cl-C )alkyl ester thereof or a non-toxic pharmaceutically acceptable salt thereof for use in the therapy of depression in the human body.

Inventors: MCDONALD IAN A, PALFREYMAN MICHAEL G, MCDONALD, IAN A., PALFREYMAN, MICHAEL G.
Applicants: MERRELL DOW FRANCE
Application year: 1982
Main classification: C07C101
IPC codes: C07C45, A61K31, C07D309